LOCUS       DAZFFI010000001       120850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFFI010000001 DAZFFI010000000
VERSION     DAZFFI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 120850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.001"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2118)
                     /locus_tag="ACHJ12_000001"
     CDS             complement(<1..2118)
                     /locus_tag="ACHJ12_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987857.1"
                     /translation="MPVVPTTSGRQVQSRGISTQGFSSFQTPNVGDVLGDVAEQYAGI
                     IAQAKQRANVAMAQDASLSLSQISSDLLNNPETGLLNLKGKNAIGKGQEYTQQFDAQV
                     EQLAMSLPDEQARNAFMQQAQQQRIQFTTQAGRHEIGQINAYEEGQFQATLLNNGKNA
                     AALYGDNAAYVLANKQTFQQIEDYGIAHGWSDEQIQAKKIEFKEKVADAALSQWSANN
                     ATAFIQSNGELSDTAAGARRAVADSDSSERARGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRQGIDTVSEIINRWAPPTDKNDTMSYIKAVCEQLG
                     VSADDPLDASNPDTLKALCAAIIHHENGSQPYSDQQLTAGVSAALGLSTIPTNTKRYT
                     GNAAFDAASPEAQASFMRQVDQLRRQQQAEYKTMIDSQVRDATAAYMRGVEFPNPPGE
                     ADFIAAYGVREGNLRYTEFRNTQIAGQYIGSFRNMPTSSITAYVEQLRPDTGETGEGY
                     AARAALYDNVVSAASQVIKQRQADPVQFSLAAGQAKPIDMSNKDNFGQSVALRAAQVS
                     DLAKSYGTPLTFFSKDEANQIGVFFRDAPVSQQAAYLDTIRQSTGGGQVYMSALQQIS
                     ANAPSAAVAGILMDKPGGILAEKNWFNPDVSVSPETAAQTILAGAAARKGTDDAKGIP
                     MPKDADLRLEFSDMVKDAFAGDAH"
     gene            complement(2131..2670)
                     /locus_tag="ACHJ12_000002"
     CDS             complement(2131..2670)
                     /locus_tag="ACHJ12_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987858.1"
                     /translation="MCNPAIALVAVTVASTAASMYSQSKQAKYQSAIADRNAEIAEAQ
                     AQDSINRGNIEADQRRREMRQRSGTAAATMGATGAELSSGTALDVFADNAQFGTLDAL
                     TTVNNAQREAYGYQVQGMNAQAQGAAAQSAAKSSMTSTLLTAPLKAYGAYQMGGGTWS
                     PFSQKAAPISAAVGTPTGR"
     gene            complement(2670..3134)
                     /locus_tag="ACHJ12_000003"
     CDS             complement(2670..3134)
                     /locus_tag="ACHJ12_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987859.1"
                     /translation="MIDVRIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALLVG
                     LRTATMAWSGFANGELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLYFHRFHMERK"
     gene            complement(3134..5605)
                     /locus_tag="ACHJ12_000004"
     CDS             complement(3134..5605)
                     /locus_tag="ACHJ12_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987860.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFLIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASSSTGTITLTASASIFGAEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTGTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGEDNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYHAEYTISVSGGAYFTSSDVGAQLQFPYTGAVPDT
                     GDEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNVATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITAEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VRIRQLDPLPLSVLAVIPRLTVGGV"
     gene            complement(5605..6210)
                     /locus_tag="ACHJ12_000005"
     CDS             complement(5605..6210)
                     /locus_tag="ACHJ12_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987861.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTDGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFAINMYNRVILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            complement(6210..6533)
                     /gene="gp10"
                     /locus_tag="ACHJ12_000006"
     CDS             complement(6210..6533)
                     /gene="gp10"
                     /locus_tag="ACHJ12_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid staple protein"
                     /protein_id="HFO4987862.1"
                     /translation="MELVNLKTGTDSYQDESGETRARDEYPWGLCITLNNDTLNKLKA
                     QPQGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            complement(6584..6919)
                     /locus_tag="ACHJ12_000007"
     CDS             complement(6584..6919)
                     /locus_tag="ACHJ12_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987863.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPSSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNDKAETLREKI
                     ADKRKELGV"
     gene            complement(6930..7367)
                     /locus_tag="ACHJ12_000008"
     CDS             complement(6930..7367)
                     /locus_tag="ACHJ12_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bbp16 family capsid cement protein"
                     /protein_id="HFO4987864.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGNRRDIGVGYPLE
                     FWVLVNEAATASGEATVNIQLQTSENNSSWTTIYDSGALAKTALAAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGKFTAGISLDVDANTPYPIRSKVTG"
     gene            complement(7419..8405)
                     /locus_tag="ACHJ12_000009"
     CDS             complement(7419..8405)
                     /locus_tag="ACHJ12_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4987865.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDANGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            complement(8420..9115)
                     /locus_tag="ACHJ12_000010"
     CDS             complement(8420..9115)
                     /locus_tag="ACHJ12_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO4987866.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPTGDNPAPVGDPS
                     QQEGDKPQPVADGDKPADDKKPESDKQGEKNDGDKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            complement(9118..9414)
                     /locus_tag="ACHJ12_000011"
     CDS             complement(9118..9414)
                     /locus_tag="ACHJ12_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987867.1"
                     /translation="MTDYEDDQLKEENARKQRDMVQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            complement(9411..11090)
                     /locus_tag="ACHJ12_000012"
     CDS             complement(9411..11090)
                     /locus_tag="ACHJ12_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO4987868.1"
                     /translation="MAETTKERLNKQFAQLESERQSFEPHWRELSDYINPRGSRFLTS
                     EVNRNDRRNTRIIDSTGTMAARTLASGMMSGITSPARPWFRLATPDPEMMDYGPVKLW
                     LEAVQNRMNDMFNKSNLYQSLPQLYGSLGTYSTGAMAVLEDDEDIIRTMPFPIGSYYL
                     ANSPRGSVDTCFRKFSMTVRQLVQEFGLNNVSESVKSMWESGTYERWIEVMHSVYPNI
                     DRDTSKLDSKNKPFKSVYYEVGGDNDKLLRESGFDEFPIMAPRWEVNGEDVYGSSCPG
                     MLALGPVKALQLLQKRKSQLIDKATNPPMVAPTSLKNQRASLLPGDITYIDQITGQDG
                     FRPAYLVNPSTADLVADIQDTRQIINSAYFVDLFMMLQNINTRSMPVEAVIEMKEEKL
                     LMLGPVLERLNDECLNPLIDRAFSMMVRKNMLPPPPDAMEGMPLKVEYISVMAQAQKS
                     IGLSSLASTVNFIGQLAQAKPEALDKLNVDQAIDAFADMSGVSPTVIVPQEQVEQARQ
                     QRAQQQQQQQMMAMGMAAAQGAKTLSEAKTSDPSVLSAMANAVSGQGGQSQ"
     gene            complement(11105..11311)
                     /locus_tag="ACHJ12_000013"
     CDS             complement(11105..11311)
                     /locus_tag="ACHJ12_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987869.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSNANTSGKTLLGQ"
     gene            11739..11815
                     /locus_tag="ACHJ12_000014"
     tRNA            11739..11815
                     /locus_tag="ACHJ12_000014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11773..11775,aa:Arg,seq:tct)
     gene            12014..12385
                     /locus_tag="ACHJ12_000015"
     CDS             12014..12385
                     /locus_tag="ACHJ12_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO4987870.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQPHESLSLLTFCVLVLRNGFTVTGESACASPENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(12476..13951)
                     /locus_tag="ACHJ12_000016"
     CDS             complement(12476..13951)
                     /locus_tag="ACHJ12_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4987871.1"
                     /translation="MTDAALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRQWQADAFREIRDHLQNPEARYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKDWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNSCKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRLPGQQNQQGKAITDYDPYA"
     gene            complement(13948..14622)
                     /locus_tag="ACHJ12_000017"
     CDS             complement(13948..14622)
                     /locus_tag="ACHJ12_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4987872.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEPKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLELIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            complement(14619..14831)
                     /locus_tag="ACHJ12_000018"
     CDS             complement(14619..14831)
                     /locus_tag="ACHJ12_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987873.1"
                     /translation="MPKIRYDLEDMRDNSANFPKEVKFLMHKYGCARRDIVIDSQHPC
                     GEDVIFIRGKWEGYLDESFYDEFDGL"
     gene            complement(14849..15187)
                     /locus_tag="ACHJ12_000019"
     CDS             complement(14849..15187)
                     /locus_tag="ACHJ12_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987874.1"
                     /translation="MAKSAAERKAAQRARQAASGVRKLEIVLDAQEIEMLERNCAARR
                     PGRAPYEFGEYIALLIRQDDARVHGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            complement(15180..15530)
                     /locus_tag="ACHJ12_000020"
     CDS             complement(15180..15530)
                     /locus_tag="ACHJ12_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HFO4987875.1"
                     /translation="MDYSQLSDFEINRMVGDIIFKGLWACKPETSGNNTNKWYYGNTD
                     TTFEPLNPLPDYCNNPSDAWPIIVDNKISLTCHHSRGEWSAVFNAENICFHANNPLRA
                     AMIVFLMMQENQNG"
     gene            complement(15530..15937)
                     /locus_tag="ACHJ12_000021"
     CDS             complement(15530..15937)
                     /locus_tag="ACHJ12_000021"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4987876.1"
                     /translation="MEVSIDVSTCDADLGNRYFGTVTEALELDTAKNGYILLVQDAEP
                     NFDVNGNSPVTPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFN
                     DGNPTWDDGDGEDLYLKEVTHWMPLPEPPQEVK"
     gene            complement(15971..16102)
                     /locus_tag="ACHJ12_000022"
                     /pseudo
     CDS             complement(15971..16102)
                     /locus_tag="ACHJ12_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(16104..16619)
                     /locus_tag="ACHJ12_000023"
     CDS             complement(16104..16619)
                     /locus_tag="ACHJ12_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4987877.1"
                     /translation="MNKIDYQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLALLDERERNQQYIKRRDQENEDIALTVGKLR
                     VELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVEVMRIAGDAQLSTPCTDSIIN
                     SIATGIRIKGE"
     gene            complement(16621..16920)
                     /locus_tag="ACHJ12_000024"
     CDS             complement(16621..16920)
                     /locus_tag="ACHJ12_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987878.1"
                     /translation="MSVYLIDKRRRGQQIPPVGIPNHTWFCVLDIDGMDALVDTRHYC
                     DTATATPAKAKKMAALIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            complement(16917..17447)
                     /locus_tag="ACHJ12_000025"
     CDS             complement(16917..17447)
                     /locus_tag="ACHJ12_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987879.1"
                     /translation="MESNIKGLVTAGHEMASELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANALAEDQQKAIESIKQADAAVKLAHEKFSALAAENELARKAVQEFCDVVGDST
                     EVICEEIGRDGVLVILEAMKATGNMPATDAFLAEVRAHGVEMFSEKFGGGTLLSNMVK
                     EVAADFAAKLRRGGNQ"
     gene            complement(17509..17853)
                     /locus_tag="ACHJ12_000026"
     CDS             complement(17509..17853)
                     /locus_tag="ACHJ12_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFO4987880.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLEQRKRAGEVAWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGFWTDDARVKTKVAADQYPFRIIGVTVKP
                     KKAGGGWNIEEF"
     gene            complement(17971..18756)
                     /locus_tag="ACHJ12_000027"
     CDS             complement(17971..18756)
                     /locus_tag="ACHJ12_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4987881.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYTNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKPEPMSTPPDTG
                     RDVYHPPGRSFGCMPNAATLGGITPAQWLMEEYRRGKAAGFIK"
     gene            complement(18753..19610)
                     /locus_tag="ACHJ12_000028"
     CDS             complement(18753..19610)
                     /locus_tag="ACHJ12_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4987882.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLV
                     KDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRVRVFSLNITKNGNIKGNREGGDE
                     PENGNVTENGNVTENGNIPKNGMLNDPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQ
                     ERESRTKSGDSVPHDPGANNAVMNNFVLPGGPGQLGKFVMHEQWQPSDDFLRKSSLQG
                     IYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWN
                     SPEAWEDFL"
     gene            complement(19626..19826)
                     /locus_tag="ACHJ12_000029"
     CDS             complement(19626..19826)
                     /locus_tag="ACHJ12_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987883.1"
                     /translation="MEIITRIDAAKRGLKRYYTGKPCKHGHDSERWVYNGHCVECTME
                     SNRRIRAEIKQIMINSSPQHSS"
     gene            complement(19977..20207)
                     /locus_tag="ACHJ12_000030"
     CDS             complement(19977..20207)
                     /locus_tag="ACHJ12_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987884.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTSKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            20362..20946
                     /locus_tag="ACHJ12_000031"
     CDS             20362..20946
                     /locus_tag="ACHJ12_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4987885.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKNKQ"
     gene            21100..21252
                     /locus_tag="ACHJ12_000032"
     CDS             21100..21252
                     /locus_tag="ACHJ12_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987886.1"
                     /translation="MQWKIINGWYCVTACGFMSWKFRTLQEGIKWAFVSKEARDVAND
                     NEIWEG"
     gene            21255..21554
                     /locus_tag="ACHJ12_000033"
     CDS             21255..21554
                     /locus_tag="ACHJ12_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987887.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLAS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDSYRREAMAIIARWDLADQIDGQRDAA"
     gene            21551..21757
                     /locus_tag="ACHJ12_000034"
     CDS             21551..21757
                     /locus_tag="ACHJ12_000034"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4987888.1"
                     /translation="MKPTLLSLLRGGKHSIRDMAKILGISRSKVSWFIAELERRKWVK
                     VTRCAIYFHDGTRSNKQNEYEVKL"
     gene            21754..22575
                     /locus_tag="ACHJ12_000035"
     CDS             21754..22575
                     /locus_tag="ACHJ12_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO4987889.1"
                     /translation="MNTGIYFDISNEDYHAGDGVSKSQLDMVAKNPALLKWVQAAPED
                     EEKKSALDMGTALHCLLLEPGEFDKRFIVSPKFDRRTKQGKADEEAFLRDVADMGITV
                     LDAEQWRKLELMRDSAMAHPAARWMLEAPGYCEASMYWNDEETGELCRIRPDKWLNEH
                     NVIVDVKKVADMDRFARHIEEFRYHVQDAMYREGAMKVTGQPHGFFFLAVSESIDCGR
                     YPVRVFELDAPDVDAGHALFRRDLNTYHECRINDEWGGVEIIKRPDWARKQDMYV"
     gene            22572..23453
                     /locus_tag="ACHJ12_000036"
     CDS             22572..23453
                     /locus_tag="ACHJ12_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecT family recombinase"
                     /protein_id="HFO4987890.1"
                     /translation="MSNDIAITSQPGATVGTAAAIFSPEGMNQLVRFAELMSQSKATV
                     PKHLEGKPADCLAVTMQAAQWGMNPFAVAQKTHVVNGTLGYEAQLVNAVVSSSSLLAT
                     RLNYRWSGDWSNVNGKTDKSPNLTVTVSAVLKGEAEPRELTISMAQAGVRNSPLWEQD
                     PRQQLAYLCTKRWARLHAPDVLLGVYTPDELQETAPRVERDITPQTTTAAGMNSLINA
                     KPAKKPDEQTRKADSRDPEEMLMAFTSAAMNYSTVSELDKAYKYIAQKLSDDDELLAK
                     ATDVYSVRREELNETSM"
     gene            23503..23751
                     /locus_tag="ACHJ12_000037"
     CDS             23503..23751
                     /locus_tag="ACHJ12_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4987891.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            23845..24096
                     /locus_tag="ACHJ12_000038"
     CDS             23845..24096
                     /locus_tag="ACHJ12_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4987892.1"
                     /translation="MRSPSKVVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            24089..24739
                     /locus_tag="ACHJ12_000039"
     CDS             24089..24739
                     /locus_tag="ACHJ12_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO4987893.1"
                     /translation="MTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPPRINAGIVQSFTAPRLKHSRKPDVVREKLVQLLGDVSRIE
                     LFARQSSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDNQGRERAA"
     gene            24736..24930
                     /locus_tag="ACHJ12_000040"
     CDS             24736..24930
                     /locus_tag="ACHJ12_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4987894.1"
                     /translation="MNRASPADLRKCLETTNMLAHSGIRFVPIPAVTDAEFATLSAIF
                     TDKIESLAAEAEMEENQQNN"
     gene            complement(24934..26184)
                     /locus_tag="ACHJ12_000041"
     CDS             complement(24934..26184)
                     /locus_tag="ACHJ12_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4987895.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKRLLKAGQSPAAAKRDGIKKIRGAETFTVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMTEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLLNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQDFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WIDGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(26377..27954)
                     /gene="guaA"
                     /locus_tag="ACHJ12_000042"
     CDS             complement(26377..27954)
                     /gene="guaA"
                     /locus_tag="ACHJ12_000042"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4987896.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(28023..29489)
                     /gene="guaB"
                     /locus_tag="ACHJ12_000043"
     CDS             complement(28023..29489)
                     /gene="guaB"
                     /locus_tag="ACHJ12_000043"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4987897.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            29651..31021
                     /gene="xseA"
                     /locus_tag="ACHJ12_000044"
     CDS             29651..31021
                     /gene="xseA"
                     /locus_tag="ACHJ12_000044"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4987898.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(31018..31233)
                     /gene="yfgJ"
                     /locus_tag="ACHJ12_000045"
     CDS             complement(31018..31233)
                     /gene="yfgJ"
                     /locus_tag="ACHJ12_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4987899.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(31302..32774)
                     /gene="der"
                     /locus_tag="ACHJ12_000046"
     CDS             complement(31302..32774)
                     /gene="der"
                     /locus_tag="ACHJ12_000046"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4987900.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(32892..34070)
                     /gene="bamB"
                     /locus_tag="ACHJ12_000047"
     CDS             complement(32892..34070)
                     /gene="bamB"
                     /locus_tag="ACHJ12_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4987901.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(34081..34701)
                     /gene="yfgM"
                     /locus_tag="ACHJ12_000048"
     CDS             complement(34081..34701)
                     /gene="yfgM"
                     /locus_tag="ACHJ12_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4987902.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(34719..35993)
                     /gene="hisS"
                     /locus_tag="ACHJ12_000049"
     CDS             complement(34719..35993)
                     /gene="hisS"
                     /locus_tag="ACHJ12_000049"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4987903.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(36104..37222)
                     /gene="ispG"
                     /locus_tag="ACHJ12_000050"
                     /gene_synonym="gcpE"
     CDS             complement(36104..37222)
                     /gene="ispG"
                     /locus_tag="ACHJ12_000050"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4987904.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(37249..38262)
                     /gene="rodZ"
                     /locus_tag="ACHJ12_000051"
     CDS             complement(37249..38262)
                     /gene="rodZ"
                     /locus_tag="ACHJ12_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4987905.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(38547..39701)
                     /gene="rlmN"
                     /locus_tag="ACHJ12_000052"
     CDS             complement(38547..39701)
                     /gene="rlmN"
                     /locus_tag="ACHJ12_000052"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4987906.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(39851..40282)
                     /gene="ndk"
                     /locus_tag="ACHJ12_000053"
     CDS             complement(39851..40282)
                     /gene="ndk"
                     /locus_tag="ACHJ12_000053"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4987907.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(40431..42743)
                     /gene="pbpC"
                     /locus_tag="ACHJ12_000054"
     CDS             complement(40431..42743)
                     /gene="pbpC"
                     /locus_tag="ACHJ12_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4987908.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(42744..47705)
                     /gene="yfhM"
                     /locus_tag="ACHJ12_000055"
     CDS             complement(42744..47705)
                     /gene="yfhM"
                     /locus_tag="ACHJ12_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4987909.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            47912..48757
                     /gene="sseA"
                     /locus_tag="ACHJ12_000056"
     CDS             47912..48757
                     /gene="sseA"
                     /locus_tag="ACHJ12_000056"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4987910.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            49093..49218
                     /gene="timP"
                     /locus_tag="ACHJ12_000057"
     CDS             49093..49218
                     /gene="timP"
                     /locus_tag="ACHJ12_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4987911.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(49250..50026)
                     /gene="sseB"
                     /locus_tag="ACHJ12_000058"
     CDS             complement(49250..50026)
                     /gene="sseB"
                     /locus_tag="ACHJ12_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4987912.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(50168..51451)
                     /gene="pepB"
                     /locus_tag="ACHJ12_000059"
     CDS             complement(50168..51451)
                     /gene="pepB"
                     /locus_tag="ACHJ12_000059"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4987913.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(51629..51829)
                     /gene="iscX"
                     /locus_tag="ACHJ12_000060"
     CDS             complement(51629..51829)
                     /gene="iscX"
                     /locus_tag="ACHJ12_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4987914.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(51841..52176)
                     /gene="fdx"
                     /locus_tag="ACHJ12_000061"
     CDS             complement(51841..52176)
                     /gene="fdx"
                     /locus_tag="ACHJ12_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4987915.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(52178..54028)
                     /gene="hscA"
                     /locus_tag="ACHJ12_000062"
     CDS             complement(52178..54028)
                     /gene="hscA"
                     /locus_tag="ACHJ12_000062"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4987916.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(54045..54560)
                     /gene="hscB"
                     /locus_tag="ACHJ12_000063"
     CDS             complement(54045..54560)
                     /gene="hscB"
                     /locus_tag="ACHJ12_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4987917.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(54656..54979)
                     /gene="iscA"
                     /locus_tag="ACHJ12_000064"
     CDS             complement(54656..54979)
                     /gene="iscA"
                     /locus_tag="ACHJ12_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4987918.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(54996..55382)
                     /gene="iscU"
                     /locus_tag="ACHJ12_000065"
     CDS             complement(54996..55382)
                     /gene="iscU"
                     /locus_tag="ACHJ12_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4987919.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(55410..56624)
                     /gene="iscS"
                     /locus_tag="ACHJ12_000066"
     CDS             complement(55410..56624)
                     /gene="iscS"
                     /locus_tag="ACHJ12_000066"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4987920.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(56736..57224)
                     /gene="iscR"
                     /locus_tag="ACHJ12_000067"
     CDS             complement(56736..57224)
                     /gene="iscR"
                     /locus_tag="ACHJ12_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4987921.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(57494..58234)
                     /gene="trmJ"
                     /locus_tag="ACHJ12_000068"
     CDS             complement(57494..58234)
                     /gene="trmJ"
                     /locus_tag="ACHJ12_000068"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4987922.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            58353..59156
                     /gene="suhB"
                     /locus_tag="ACHJ12_000069"
     CDS             58353..59156
                     /gene="suhB"
                     /locus_tag="ACHJ12_000069"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4987923.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            59301..60155
                     /gene="yfhR"
                     /locus_tag="ACHJ12_000070"
     CDS             59301..60155
                     /gene="yfhR"
                     /locus_tag="ACHJ12_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4987924.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            60346..61626
                     /gene="csiE"
                     /locus_tag="ACHJ12_000071"
     CDS             60346..61626
                     /gene="csiE"
                     /locus_tag="ACHJ12_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4987925.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(61618..62757)
                     /gene="hcaT"
                     /locus_tag="ACHJ12_000072"
     CDS             complement(61618..62757)
                     /gene="hcaT"
                     /locus_tag="ACHJ12_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4987926.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(62917..63807)
                     /gene="hcaR"
                     /locus_tag="ACHJ12_000073"
     CDS             complement(62917..63807)
                     /gene="hcaR"
                     /locus_tag="ACHJ12_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4987927.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            63943..65304
                     /gene="hcaE"
                     /locus_tag="ACHJ12_000074"
     CDS             63943..65304
                     /gene="hcaE"
                     /locus_tag="ACHJ12_000074"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4987928.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            65301..65819
                     /gene="hcaF"
                     /locus_tag="ACHJ12_000075"
     CDS             65301..65819
                     /gene="hcaF"
                     /locus_tag="ACHJ12_000075"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4987929.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            65819..66139
                     /gene="hcaC"
                     /locus_tag="ACHJ12_000076"
     CDS             65819..66139
                     /gene="hcaC"
                     /locus_tag="ACHJ12_000076"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4987930.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            66136..66948
                     /gene="hcaB"
                     /locus_tag="ACHJ12_000077"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             66136..66948
                     /gene="hcaB"
                     /locus_tag="ACHJ12_000077"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4987931.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            66958..68160
                     /gene="hcaD"
                     /locus_tag="ACHJ12_000078"
     CDS             66958..68160
                     /gene="hcaD"
                     /locus_tag="ACHJ12_000078"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4987932.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            68257..68679
                     /gene="yphA"
                     /locus_tag="ACHJ12_000079"
     CDS             68257..68679
                     /gene="yphA"
                     /locus_tag="ACHJ12_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4987933.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNLINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(68727..69599)
                     /gene="yphB"
                     /locus_tag="ACHJ12_000080"
     CDS             complement(68727..69599)
                     /gene="yphB"
                     /locus_tag="ACHJ12_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4987934.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLMVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(69611..70705)
                     /gene="yphC"
                     /locus_tag="ACHJ12_000081"
     CDS             complement(69611..70705)
                     /gene="yphC"
                     /locus_tag="ACHJ12_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4987935.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(70738..71736)
                     /gene="yphD"
                     /locus_tag="ACHJ12_000082"
     CDS             complement(70738..71736)
                     /gene="yphD"
                     /locus_tag="ACHJ12_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4987936.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(71761..73272)
                     /gene="yphE"
                     /locus_tag="ACHJ12_000083"
     CDS             complement(71761..73272)
                     /gene="yphE"
                     /locus_tag="ACHJ12_000083"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4987937.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(73295..74278)
                     /gene="yphF"
                     /locus_tag="ACHJ12_000084"
     CDS             complement(73295..74278)
                     /gene="yphF"
                     /locus_tag="ACHJ12_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4987938.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(74375..77656)
                     /gene="yphG"
                     /locus_tag="ACHJ12_000085"
     CDS             complement(74375..77656)
                     /gene="yphG"
                     /locus_tag="ACHJ12_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4987939.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPNAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            77774..78967
                     /gene="yphH"
                     /locus_tag="ACHJ12_000086"
     CDS             77774..78967
                     /gene="yphH"
                     /locus_tag="ACHJ12_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4987940.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(79031..80284)
                     /gene="glyA"
                     /locus_tag="ACHJ12_000087"
     CDS             complement(79031..80284)
                     /gene="glyA"
                     /locus_tag="ACHJ12_000087"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4987941.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            80613..81803
                     /gene="hmpA"
                     /locus_tag="ACHJ12_000088"
     CDS             80613..81803
                     /gene="hmpA"
                     /locus_tag="ACHJ12_000088"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4987942.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(81848..82186)
                     /gene="glnB"
                     /locus_tag="ACHJ12_000089"
     CDS             complement(81848..82186)
                     /gene="glnB"
                     /locus_tag="ACHJ12_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4987943.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(82247..83581)
                     /gene="glrR"
                     /locus_tag="ACHJ12_000090"
     CDS             complement(82247..83581)
                     /gene="glrR"
                     /locus_tag="ACHJ12_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4987944.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(83571..84284)
                     /gene="qseG"
                     /locus_tag="ACHJ12_000091"
     CDS             complement(83571..84284)
                     /gene="qseG"
                     /locus_tag="ACHJ12_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4987945.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(84449..85876)
                     /gene="qseE"
                     /locus_tag="ACHJ12_000092"
     CDS             complement(84449..85876)
                     /gene="qseE"
                     /locus_tag="ACHJ12_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4987946.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(86452..90339)
                     /gene="purL"
                     /locus_tag="ACHJ12_000093"
                     /gene_synonym="purI"
     CDS             complement(86452..90339)
                     /gene="purL"
                     /locus_tag="ACHJ12_000093"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4987947.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            90597..92153
                     /gene="mltF"
                     /locus_tag="ACHJ12_000094"
     CDS             90597..92153
                     /gene="mltF"
                     /locus_tag="ACHJ12_000094"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4987948.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(92150..92653)
                     /gene="tadA"
                     /locus_tag="ACHJ12_000095"
     CDS             complement(92150..92653)
                     /gene="tadA"
                     /locus_tag="ACHJ12_000095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4987949.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(92711..93346)
                     /gene="yfhb"
                     /locus_tag="ACHJ12_000096"
     CDS             complement(92711..93346)
                     /gene="yfhb"
                     /locus_tag="ACHJ12_000096"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4987950.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            93555..94403
                     /gene="yfhH"
                     /locus_tag="ACHJ12_000097"
     CDS             93555..94403
                     /gene="yfhH"
                     /locus_tag="ACHJ12_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4987951.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            94459..94719
                     /gene="yfhL"
                     /locus_tag="ACHJ12_000098"
     CDS             94459..94719
                     /gene="yfhL"
                     /locus_tag="ACHJ12_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4987952.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(94913..94993)
                     /gene="shoB"
                     /locus_tag="ACHJ12_000099"
     CDS             complement(94913..94993)
                     /gene="shoB"
                     /locus_tag="ACHJ12_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4987953.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(95413..95793)
                     /gene="acpS"
                     /locus_tag="ACHJ12_000100"
     CDS             complement(95413..95793)
                     /gene="acpS"
                     /locus_tag="ACHJ12_000100"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4987954.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(95793..96524)
                     /gene="pdxJ"
                     /locus_tag="ACHJ12_000101"
     CDS             complement(95793..96524)
                     /gene="pdxJ"
                     /locus_tag="ACHJ12_000101"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4987955.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(96536..97264)
                     /gene="recO"
                     /locus_tag="ACHJ12_000102"
     CDS             complement(96536..97264)
                     /gene="recO"
                     /locus_tag="ACHJ12_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4987956.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(97276..98181)
                     /gene="era"
                     /locus_tag="ACHJ12_000103"
     CDS             complement(97276..98181)
                     /gene="era"
                     /locus_tag="ACHJ12_000103"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4987957.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(98178..98858)
                     /gene="rnc"
                     /locus_tag="ACHJ12_000104"
     CDS             complement(98178..98858)
                     /gene="rnc"
                     /locus_tag="ACHJ12_000104"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4987958.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(99131..100105)
                     /gene="lepB"
                     /locus_tag="ACHJ12_000105"
     CDS             complement(99131..100105)
                     /gene="lepB"
                     /locus_tag="ACHJ12_000105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4987959.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(100121..101920)
                     /gene="lepA"
                     /locus_tag="ACHJ12_000106"
     CDS             complement(100121..101920)
                     /gene="lepA"
                     /locus_tag="ACHJ12_000106"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4987960.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(102118..102597)
                     /gene="rseC"
                     /locus_tag="ACHJ12_000107"
     CDS             complement(102118..102597)
                     /gene="rseC"
                     /locus_tag="ACHJ12_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4987961.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(102594..103550)
                     /gene="rseB"
                     /locus_tag="ACHJ12_000108"
     CDS             complement(102594..103550)
                     /gene="rseB"
                     /locus_tag="ACHJ12_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4987962.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(103550..104200)
                     /gene="rseA"
                     /locus_tag="ACHJ12_000109"
     CDS             complement(103550..104200)
                     /gene="rseA"
                     /locus_tag="ACHJ12_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4987963.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(104233..104808)
                     /gene="rpoE"
                     /locus_tag="ACHJ12_000110"
     CDS             complement(104233..104808)
                     /gene="rpoE"
                     /locus_tag="ACHJ12_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4987964.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(104805..104960)
                     /gene="rseD"
                     /locus_tag="ACHJ12_000111"
     CDS             complement(104805..104960)
                     /gene="rseD"
                     /locus_tag="ACHJ12_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4987965.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            105216..106833
                     /gene="nadB"
                     /locus_tag="ACHJ12_000112"
                     /pseudo
     CDS             105216..106833
                     /gene="nadB"
                     /locus_tag="ACHJ12_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            complement(106818..107555)
                     /gene="trmN"
                     /locus_tag="ACHJ12_000113"
     CDS             complement(106818..107555)
                     /gene="trmN"
                     /locus_tag="ACHJ12_000113"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4987966.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            107687..109021
                     /gene="srmB"
                     /locus_tag="ACHJ12_000114"
     CDS             107687..109021
                     /gene="srmB"
                     /locus_tag="ACHJ12_000114"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4987967.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(109231..110112)
                     /gene="yfiE"
                     /locus_tag="ACHJ12_000115"
     CDS             complement(109231..110112)
                     /gene="yfiE"
                     /locus_tag="ACHJ12_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4987968.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            110215..110802
                     /gene="eamB"
                     /locus_tag="ACHJ12_000116"
     CDS             110215..110802
                     /gene="eamB"
                     /locus_tag="ACHJ12_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4987969.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(110858..111241)
                     /gene="grcA"
                     /locus_tag="ACHJ12_000117"
     CDS             complement(110858..111241)
                     /gene="grcA"
                     /locus_tag="ACHJ12_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4987970.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            111546..112235
                     /gene="ung"
                     /locus_tag="ACHJ12_000118"
     CDS             111546..112235
                     /gene="ung"
                     /locus_tag="ACHJ12_000118"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4987971.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(112283..113320)
                     /gene="yfiF"
                     /locus_tag="ACHJ12_000119"
     CDS             complement(112283..113320)
                     /gene="yfiF"
                     /locus_tag="ACHJ12_000119"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4987972.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            113527..113946
                     /gene="trxC"
                     /locus_tag="ACHJ12_000120"
     CDS             113527..113946
                     /gene="trxC"
                     /locus_tag="ACHJ12_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4987973.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            114015..114713
                     /gene="tapT"
                     /locus_tag="ACHJ12_000121"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             114015..114713
                     /gene="tapT"
                     /locus_tag="ACHJ12_000121"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4987974.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            114745..117405
                     /gene="pat"
                     /locus_tag="ACHJ12_000122"
     CDS             114745..117405
                     /gene="pat"
                     /locus_tag="ACHJ12_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4987975.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            117519..118874
                     /gene="pssA"
                     /locus_tag="ACHJ12_000123"
     CDS             117519..118874
                     /gene="pssA"
                     /locus_tag="ACHJ12_000123"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4987976.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            118920..119243
                     /gene="yfiM"
                     /locus_tag="ACHJ12_000124"
     CDS             118920..119243
                     /gene="yfiM"
                     /locus_tag="ACHJ12_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4987977.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(119240..120538)
                     /gene="kgtP"
                     /locus_tag="ACHJ12_000125"
     CDS             complement(119240..120538)
                     /gene="kgtP"
                     /locus_tag="ACHJ12_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4987978.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(120655..>120850)
                     /locus_tag="ACHJ12_000126"
     CDS             complement(120655..>120850)
                     /locus_tag="ACHJ12_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4987979.1"
                     /translation="KAISHDGLFALAQKKMPDATLRVLYSHICQIQQRMRFPQLAYFH
                     TCPPYQKFILKLLNNHFPAN"
BASE COUNT        28795 a        32707 c        30010 g        29338 t
ORIGIN      
        1 atgagcatca ccagcaaatg catccttcac catgtcagaa aactcaaggc gaagatcagc
       61 atctttaggc atcggaatac ctttcgcgtc atcagtacct ttacgagccg ccgcgccagc
      121 aagaattgtc tgcgcagcgg tttctggaga cacggaaaca tccggattaa accagttttt
      181 ttctgccaaa ataccacctg gcttatccat cagtatcccg gcaacggcag cagatggagc
      241 gttggcactg atctgctgta gtgctgacat atacacctgc ccaccaccag tgctctgcct
      301 gatggtatcg agatatgctg cctgttggga aacgggcgca tcacgaaaga aaacaccgat
      361 ctgattggcc tcgtctttgg aaaagaacgt cagtggagtg ccatatgact tagcaaggtc
      421 actgacctga gcagcacgca aggcaacgct ctgtccaaag ttatccttat tgctcatgtc
      481 gataggcttt gcctgtccgg cggcaagaga gaactgtaca ggatcagcct gtctctgctt
      541 tatcacctga cttgcagccg acacaacgtt gtcataaaga gcggctcgtg ccgcataacc
      601 ctcccctgtc tcaccagtat ccgggcgtaa ttgctcaaca tatgctgtaa tgctgcttgt
      661 cggcatgttg cggaaagagc ctatatactg tccggcgatt tgcgtatttc tgaactcggt
      721 atatcgcagg tttccttctc tgactccata agctgcaata aaatcagcct caccaggtgg
      781 gttaggaaat tcaacgccac gcatatacgc agctgtcgca tcgcgaactt ggctgtcaat
      841 catcgtttta tattcagcct gctgctgccg acgcagttga tccacctgtc gcataaaact
      901 tgcctgcgcc tcaggagatg ccgcatcgaa tgctgcatta ccggtatagc gtttggtgtt
      961 ggttggaatt gttgataaac caagtgctgc actgacacca gcagttaact gctgatcact
     1021 gtatggctgg ctaccgttct catgatggat aatggctgca caaagcgcct tcagggtatc
     1081 aggattagat gcatcgagag gatcatcagc agaaacgcca agttgttcgc acactgcttt
     1141 gatatacgac atagtatcat ttttatcagt aggcggtgcc cagcgattaa ttatctcgct
     1201 gacggtatca ataccctgcc tctgatacga catcaggttt cgccctaatg cacgaatccc
     1261 gtgttcaggt gtttcgaatt tagcaaatcg accatcatca ccggtctggc ctacccacgg
     1321 attagttttg ctgtattcga gatttcctgg gttattgttg cgtatgccac gggcacgctc
     1381 ggaagagtca ctatctgcta cagcacggcg agctccagca gcagtatcac ttaactcacc
     1441 attactttgg atgaatgcgg tcgcattgtt tgccgaccac tgggacaatg ctgcatcagc
     1501 aaccttctct ttaaactcga ttttcttggc ctggatttgc tcgtcgctcc agccatgcgc
     1561 aatgccgtaa tcctcaattt gctggaaagt ttgcttatta gccaatacgt atgcggcgtt
     1621 gtcgccatac aatgctgcgg catttttacc attgttcagc agcgtcgcct gaaactggcc
     1681 ttcttcgtag gcatttattt gccctatctc gtgccgcccg gcctgcgtag tgaactgaat
     1741 gcgctgctgc tgcgcctgct gcatgaaagc attacgagcc tgttcatccg gcagcgacat
     1801 agccagttgc tcgacctggg catcaaactg ctgcgtatac tcctgacctt ttccaatagc
     1861 atttttccct ttcaggttaa gcaaacctgt ttcagggtta ttcagcagat cgctgcttat
     1921 ctggcttaag ctaagagaag catcctgagc catagcaaca ttcgcacgct gttttgcctg
     1981 cgcaataata cctgcatatt gctctgcaac atcgccaagt acatcaccga catttggtgt
     2041 ctgaaacgat gagaatccct gcgtcgaaat ccctctgctc tgaacctgac ggcccgatgt
     2101 tgttggtaca actggcatct tattatcccc ttatcgaccg gttggagtgc caacagcagc
     2161 agaaatcggc gcagccttct gagagaatgg gctccacgtt ccaccgccca tctggtatgc
     2221 accgtatgct tttagtggtg ccgttaacaa agtgctggtc atcgatgatt tagcagccga
     2281 ctgagcagca gccccctgtg cctgagcatt cattccctga acctgatacc catatgcctc
     2341 acgctgagca ttattcactg tcgttaacgc atcaagagtg ccgaactgag cattatccgc
     2401 aaaaacgtca agagctgttc cgctacttaa ttccgcaccg gtagccccca tagtggccgc
     2461 cgcagtgcct gagcgttgac gcatttcacg acgacgctga tccgcttcaa tattcccacg
     2521 attgattgaa tcctgtgcct gagcttcagc aatttcagca ttccgatcag ctatggctga
     2581 ctggtatttt gcctgcttgc tctggctgta cattgacgcg gctgtggatg ccactgtgac
     2641 ggcaaccaaa gcgatggctg ggttacacat tattttctct ccatgtgaaa tctgtggaaa
     2701 taaagaccaa gagctccata aggcgcggct tcttcaagcc tgaatccaag ccagtgcagc
     2761 catgctttgg caacatggtt tcgctcgtcg acgtagtttt ccaggcgcgg ataaactgcc
     2821 agcatctgct gcaatacagg gcggcagtgg cgcagaaatg tcttctgata tttttcaata
     2881 cggctggttc ctaccagcca gggcgtacca ttgccaccga tcattgacgc cggagataca
     2941 ccaaacatgg ttaccagttc tccgttcgcg aaccctgacc aggccatagt cgcagtgcga
     3001 agaccaacaa gcagcgcatc ttcggtagtc atcagcgata ccgcatacag ttcgtcaata
     3061 tcagcctgac gaacatccgg caaaatcatc tgaagatgct cttcggtggc gggaataatt
     3121 cgaacatcga tcatcagacc cccccaacag taaggcgagg aataacggca agaacagaca
     3181 gcggcaacgg gtcaagctga cggattctta cacgtccgtt tttgccccag ttactgtcca
     3241 gtttcacttc tacttttccg gtagcgtcat caacaggatc atcgtagaac tcgaattcac
     3301 gctgtggata ttcgtaccat ttaccgccgg gcgtagtcgc ccagatgccg cgactggcat
     3361 tcacaaccag agtaacggac gggatcacct gttttttgtc cagcagcgtt tcctgtccgt
     3421 taatgttgat atccagtgtt tcgaattcag cagttattgg caggccgatg tgcactacag
     3481 cccccggtga ttccagcgtg acggcacctc cggaaacaac tttctgtggt tccacgttcg
     3541 catcagagag aatgtttacg gtctggcctt caagatgaga caggcctcca aatgtccggc
     3601 gcgccatctg ccagttcgtg gtggccacat tcctgaggga tggcgggacg ttcctgttag
     3661 cacgaaccac tactgcggta ttgctggtta cagaaataat gtcgcaacgt aattcttttg
     3721 acacttcatc gccagtatca ggaacagctc cggtataagg gaactgtagt tgcgcaccaa
     3781 catcactact ggtgaagtac gcaccaccag aaacactgat tgtatattcc gcgtggtaat
     3841 cccattcacc agaaccacca gtgatggtca tcgttctgtc agacgtattt cttccatcat
     3901 agctaaggcc agaatcaaca aagaaagcat cttcatcgct ggtaaataaa cggctggaca
     3961 gtctctcgat gtatctcact gtttgcccgt taacggttct gttaacgacg aaatacaccg
     4021 catcttcatt gccttcgctg atactgcatg tgctttcata ttttccggta ctggattgtg
     4081 gtgcccatgc aaaaacctgc tgatcacgca aataggtcat caccagtaat ttaccgtcat
     4141 cacgaatgca gaaggcactg gagtaaggga caatcgagaa gcaccagtca acaatgctgt
     4201 gcttctgaaa aagatgattg gcaaggatag tcaggtcgtt cccctgatag ccgtcaacat
     4261 cgaatgaata ggccagatca cggacaacac tgcctttctc ctggacgaac agagcaatat
     4321 tcgccacagc aattggtggg acgttgcttg agccatttga tccctgagag ctgaatgcaa
     4381 atgatgatgg ggttaacact ttgttctggt cgccggtgat gacgtactca cctccggaag
     4441 tcagcgccac cagagaacca acatcaatca ggtgacggat ctcattaacc tgacgcccag
     4501 cataggtgta gataattctg tcgtcatcct gcgtaggatt gcttttgcca aaatccttat
     4561 aatccccggt acggctggcc cagatagtct gagggaacgc agtcgatgcg gcgaagtaaa
     4621 gacgttgttg ataataaaca acagtgccag gataaccatt aacactgttc caggcatatt
     4681 tagcccattt atagctggca ttatcctcgc caacgacctg cgaagggata taggaaatca
     4741 cctcagcagt tgcagtagtg ccatttgcag cagtgatacg ggcaatgcca aaaccactgt
     4801 gcagatattc ccactcaatg ccagtatcat catcaccgga tccgccccag ccatcccatg
     4861 atgtgccttc tgtatgcgaa gggcgcaaag tacctgtttt tcctgctgta acggcgcgat
     4921 agtagttact gtctgcacgg cgaatatcgc caatcgacgt actcttactg gtttcccata
     4981 ccggtactga atccactgct ggctgttcca gatagaacaa tttgcctacc tgctccgcgc
     5041 caaaaataga ggcgcttgcc gttaacgtaa ttgtcccggt gctggagctg gcataaaccg
     5101 tcactgactc gtcaatattg atatcttcaa atggcccgtt cttcgttacc acatcaacca
     5161 gttgccagtt gtcatgcgca taacgtcgca actctttcgg cgggtatgcc ggatgaacca
     5221 gcgtaagcac gtctgcgctt tgcgtgaatt taattaggaa cagatcggct tcagtatatg
     5281 gcgtggcaat ttcataaata acattactgc tgttcagcac caacgcacca tctttgataa
     5341 cacgcatgta ctggtgtccg aactccagag cataagtctg aaccgtcgag aactggaacg
     5401 ggatcaggcg gcatttccga tttgggtatt tggcggcacc gacaaaacgc gtaccaggtc
     5461 gattctcaac gccgccatac tgccgcacga taaagttatc gcacttgcgc aatgccacct
     5521 ggtacttcgc catgtcgata cgtccgtaca acgacggtcc aatctcacca ccggcaaagc
     5581 tgggctggat ccaactgata gccatcagga caacctcgca atggtaaact catcaaccgg
     5641 tggctgtggt tcctgtgatt cattctggct atgcgagcca gcactaagaa tcacgcgatt
     5701 gtacatattg atggcaaacg taccgaggtc tgcattccca gtcagcgcca tgttaatagc
     5761 tgccgcaaga cgccaggcca acgcctccat aaaaatggca tcaaacatgt tcacatctga
     5821 aacgcgagag acatacttga gccatgcctg cggctggtct gtgtagatca actttcctgt
     5881 tccgtcggtg tctgcaccaa cttcgtactg aacgcgcatt gctgctgttg gattgcgtac
     5941 accaggaagc ataatttcag taatgcgcag acaatcggac gggtactggt acgcatattc
     6001 ccagtcaggc ggtggattgc tcgtatctgc aagcgccacg cgtttggtag caaagttcca
     6061 gtcaaaatca gaaagcacag catcacggca ggcctcaaag tgcagcgaac attcccccgc
     6121 ttccttgctg gcttccgtca ggctgttaat gctgcggcta ttgccaatat tggacagcgc
     6181 acgattacag atctctacta cagaggccat cactcacctc cgttaccgta cagagtttca
     6241 gccgctgatt tttctacatc cccggaaaca ggagcgatcg ccatatcagt gatctgcaga
     6301 tcggcgctgc gattaacgcc atcgtcagtt tctctggcag acaggcctcg aataacagcc
     6361 tttgcagtta tcatcacttc tgttccgacg ccctgaggtt gcgccttcag cttattcaat
     6421 gtgtcgttat taagagtgat gcacagcccc cacgggtatt catcgcgagc tctggtttct
     6481 ccgctctcat cctggtagct gtcagtgccg gttttgaggt ttacgagttc catatacact
     6541 cctgcaataa aggggccgaa gccccttgtc tgattcgcga ggcttacacg cccagttctt
     6601 tacgcttatc tgcgatcttc tcgcggagcg tttcggcttt gtcgttatgg tgtggcttct
     6661 cgttaaagag caattcgtac tcttcacgga gcttatccag ttcaccatca tctgacacat
     6721 cgttgatgat tttggtgctg gttgctgcca ttgacacctt tcctgcaact tttgcttttg
     6781 cctgtctggc tgcatcgtta acaggttcca gtgcgctact aggctcacct tcgtattcga
     6841 tttctgcccc ctccggccac agagtgttat ggatatgaga gaggcgcaga acgcggtatc
     6901 ttggtttctc acctgacatc gatatcacct taaccagtta cttttgagcg gatcgggtac
     6961 ggcgtattgg catcaacatc cagactgata cccgcagtga atttgccggc cgttagtggg
     7021 ccagttgcga cggagtagtt aacacgcaga tatcgctgaa caccggcagg cacctttgca
     7081 gaaacaactc gtttacctgc tgccagggcg gtctttgcca gtgcgccact atcataaata
     7141 gtggtccatg agctgttatt ctcactcgtc tgcaactgga tgtttacagt tgcctcacca
     7201 cttgccgtgg cggcttcgtt aaccagcacc caaaactcaa gcgggtaacc cacaccgata
     7261 tcgcgacggt ttccatcaat tggaccgaga tcgattacgt cagtagaagc cgcggtattc
     7321 gtaaccgcct gagcttcgga gaacatcaac agtttgtcgg tgatcatctt ctttctccat
     7381 tagtgggtct gttacgaccc acaggttaat aacaggcgtt acaccacgcg ggcttctgtt
     7441 tccagaagcg catcagtttc acggattggt acaccacgga atgaagtcca ccactcgcct
     7501 tctgtctctt ttacgctgat cgccagagat gttttctcca gagattgcag atcaagagcc
     7561 tggcctacag tgcggttcat gtagaacacc gggcgaccca tgccacgatt tggaatgcga
     7621 tgcagtgctt taaccatcaa cttcgcaata tttgcggcag aggaaggttc tgaaagattg
     7681 ctgacatcga tgtttgcaat gcgaacaaca taacgccagt cacgcagagc aagtccgtta
     7741 tcccatttgt aatgggtgcg atagccttcg tacttgccgc cattagcatc ttccagtgtc
     7801 acctggcctt tatcttccat ctggatgcca gccttctgcc ctttcgggaa gatgccatgc
     7861 acggtgtttt cgccccacac cactaaccag attgaggtgt tatctgtacc cgtgccacca
     7921 gcatcaatga tgttctgagc attacccgca gacaggctgg aatagcggga ggacagtccc
     7981 ataaactgct gagggttaac gctggaatca ccataaaaca gcgtctgcgc catctgctga
     8041 ttcatcgctt caataaatgc gcggtcttca gacaggcgga attcggcggt attgccgttc
     8101 agatcagcca gtgacttatc gacttccgca taggtttcca gcatgccaac ggaatcggtt
     8161 acctgcactg tggttgattt gcttggctgt acgccatagt tcagcaaacg ccaggtagct
     8221 gaaggtaaac cagaacgaat ggtggttcgg tgtccggtag gaaggttccc ttcgacaaaa
     8281 ggcatatcct gaaggatcgg gttagtttga ccgagaagct cgataatctt atcgactttc
     8341 ccgtttggat cgacgcgctt accccagtca gccagcgtta gcgcagttaa gcctttaaca
     8401 gccattgtca tttcctcttt tatttgccat agagcacttc ggccgcacta cgctggcctt
     8461 cattaccacc ggtgaccatg ccatcttcag acatcgcctt tccgattttc acgaacgttt
     8521 tgaccagatc agggtgatta cccagcccgg tggtgttcag atattctttg agttcaggtg
     8581 tcccgaactg gtcaagcgca cgctgtgcgg cgctaaggtt agaaatcaac ttgtcgccac
     8641 cgatttcttt gtcagctttt acatccgcag cccactgctc ggttgttttc tgccaggctt
     8701 ctgcctggcg ctgctgaaca cctgccagaa tcttcggata agcatcaacc agcttttgcg
     8761 cttgctcgtt ggtcaggtta agttctcgcg ccaccggctc gaattccttc aacgcttctg
     8821 tatccagctc tacgccttcg gcagcctgaa actcgtactt ctccggcgca ccctccggtt
     8881 tatcgccgtc atttttttca ccctgcttat cgctttcagg ctttttgtca tcagcaggtt
     8941 tatcgccatc agcaacaggt tgtggcttat caccttcctg ttgtgatgga tcaccaactg
     9001 gagcagggtt atcacctgta ggcgctgacg gttctgacgc agccggagct gctccaccat
     9061 cgactggttg ctcattgcaa agacggcgat acagcaaacg ctcaaataaa ttcatgatca
     9121 ctcctgttca ctggcctctt tggccatctt caaatactgc tcagggcaat gcgccataac
     9181 gcgctgaaac agttccagcg ccagattgcg ttgcccctca ttaaatgcca ttgccatagc
     9241 gtccatcggt gagatagcgg aaaacacacg gcctttctcc agcaccgacc agacaacgcg
     9301 acgcccctgt tcactgctca tgacaaagcg aatgtcatca atttcacgct gcaccatgtc
     9361 acgttgctta cgggcgtttt cttctttcag ttgatcgtct tcgtaatctg tcattgtgat
     9421 tgcccaccct gaccactaac tgcattcgcc atagctgaca aaacactcgg atccgaagtt
     9481 ttagcttcac ttagcgtctt ggcaccctgt gccgccgcca tccccatcgc catcatttgt
     9541 tgctgctgtt gttgctgtgc ccgttgctgg cgagcctgct caacctgttc ctgcggaaca
     9601 atgacggttg gagacactcc ggacatatca gcgaatgcat cgatcgcctg atcaacgttg
     9661 agtttgtcga gagcttctgg tttcgcttgc gcaagttgac caatgaagtt aaccgtggac
     9721 gccagactgg acaggccgat agacttctgc gcctgagcca tgacggaaat gtattcgacc
     9781 ttcaggggca tgccttccat cgcgtcaggc ggtggcggca gcatgttttt acgcaccatc
     9841 atagagaaag cgcggtcaat gagaggatta agacattcgt cgttcagacg ctccagaacc
     9901 ggccccaaca tcaggagttt ttcttctttc atttcgatca ccgcttcaac aggcatcgag
     9961 cgggtattga tgttctgcaa catcatgaac agatcgacaa agtaggcgct gttaatgatt
    10021 tggcgagtgt cctgaatgtc tgccaccaga tctgctgtac tggggttaac cagataagca
    10081 ggcctgaaac catcctgacc agtaatctga tcgatatacg tgatgtcgcc aggaagaagg
    10141 gaggcgcgct gattcttgag ggaagtcgga gcaaccatcg gcggattggt ggctttatca
    10201 atcaactgcg acttgcgctt ctggagaagc tgcaatgcct taacaggtcc aagcgccagc
    10261 atacccgggc atgatgatcc ataaacatct tcgccgttaa cttcccagcg cggagccata
    10321 attggaaact catcgaatcc ggactcacgc aacaacttgt cgttatcgcc accaacctcg
    10381 taataaaccg atttgaatgg cttgttcttg ctatccagct tcgatgtatc gcggtcaatg
    10441 ttcgggtaaa ccgaatgcat cacttcaatc cacctctcgt aggtgccgct ttcccacatg
    10501 ctttttacgg attcgctgac gttatttagt ccgaattcct gaacaagctg acgaacagtc
    10561 atagagaact tgcgaaaaca ggtgtccaca ctgccacgag gtgagttagc caggtagtaa
    10621 cttcctatcg ggaatggcat tgtacgaatg atgtcctcgt catcctccag caccgccatt
    10681 gcaccggtgc tgtatgtgcc gaggcttccg tataactgcg gcagcgactg atagagattc
    10741 gacttattga acatatcgtt catgcggttc tgcactgcct caagccacaa cttaacaggg
    10801 ccataatcca tcatttcagg atctggcgta gccaggcgaa accacggacg cgcggggctt
    10861 gtgatgcctg acatcatgcc gctggcgaga gtgcgcgccg ccatagtccc ggtcgaatca
    10921 ataatgcgtg tattgcgtcg atcgttacgg ttgacctcag aagtcagaaa gcgggaacca
    10981 cgcgggttga tgtaatcact caactcgcgc cagtgcggct cgaacgactg acgctcgctt
    11041 tcaagttgtg cgaactgttt gttcaatcgc tctttagttg tttccgccat ttcaatgact
    11101 ccggttactg accaagcagc gttttaccgc tggtattagc gtttgatgtg tcgccctgag
    11161 aaccggtaag cagcgtagaa ctacgaccag cagcagcgcg acggcgacgt gtttcttcgt
    11221 cgcgggcatc aacaacggcg gcatcctgct cctgtggtgc tgcctgaact tctggtgttg
    11281 caggcactga tggtgagcta cccatgcaca tatcaatgac tccgtacgca attaaattat
    11341 taccaattta accacatatg atttatttat cgtagatagt tgacatttaa cgcacgaatt
    11401 attacctttc aggtaagcaa agagttcatt ccggttatta acctgactgg cttgtcgtta
    11461 aattgaacag gtggagtgag cttttatttt gagcagtacg gcgtatggca catgcgccga
    11521 tagcggtctg gatacgttta aggggcaccc tcccttgctc gagcaaacga accaggtagc
    11581 cggaatgtgc aagtcgagcg gttttattcc gcgcacaggg attcaccatc ccggcgattc
    11641 ggtgtgacgc ctcggaagag acgagggtac aacgatgaga gcatttatgg agccgcgaca
    11701 aagtgtggcg ccttaacagg ctaagtgctc tcagcgttgt ggcattagct cagttggaca
    11761 gagcaaccgc cttctaagcg gttggtcgca ggttcgaatc ctgcatgcca cgccagaatc
    11821 acgcctaagg accgtgatgc cagaagttcc aggggcttgg cggtgatggt ttcccttgaa
    11881 ggactatcac cgcccttttt acagcaggac gccattgcga tgacttcatg ctgtaaacca
    11941 gtacagccac ggaaggcata actcattgct tccagttcgc ccggttcgcc gggcattttt
    12001 ttaaggtgag attatgaacg accagcaaat cgaaaaagaa atcgttgaga aaggcaaaac
    12061 ggcaccgcgc gttacgccag accatatcga aggcattatt gctcaggagg catatttcac
    12121 agcagaagat ggtgcctttg gcaaagccat aaaagcgaaa catactggcg gagaggtaaa
    12181 ctaccagccg cacgaatcac tttctctgct gacgttctgc gtcctggtgc tgcgcaacgg
    12241 cttcaccgtc accggagaga gcgcctgtgc aagcccggaa aactttgatg cagaaattgg
    12301 tcggaagatt gcccggcaga atgctgtaaa caaaatctgg atgctcgaag gttacttgct
    12361 gaagcagaag ctaagcgagc aataacaccg tgacatctca caaacagcca gccgatgagc
    12421 tggctttgtt ttatcctcat cagaggatat caacgacatt atccccacca gcggattaag
    12481 catacgggtc ataatctgtg atggccttgc cttgctggtt ctgctgcccg ggaagtcgca
    12541 ggcgcttcga caccgggaaa gcaaacgtca gcagtagcgc atcgccttta ccaggcgaac
    12601 gcccaagccg ctccttgata tcttccttcg gttcgataac gattttaccg tccactcgaa
    12661 ctttgtactc tgccgccgac agatcgtccg ctgtttcctg gtcatccagc atcccgccca
    12721 gcctcagcca tgtcttgcat gagttgaaca tctccccacg cttgttgagc atctgcgggt
    12781 cagtagacgc gccaccgaac ggaacaagtt gccatgtgcg cccccaaccg tcaccgattg
    12841 acttcagacc ggtaccgtaa ccaaagtcga tgaacactgc gtcagcctga tactggtctt
    12901 caaagtcagc gatacgcttc gccataatca gatcgtcggt agtcttgttg ccagtccaca
    12961 gcaccttact gtgcagcccc tgccgcaggt atatcaccgc gtcatcaaca ccggagtatg
    13021 cagggtcaac gccgattatc accggagcat gtgccacctg cgcagcagtt accacccgtt
    13081 tcattgcctc gtcagtaaga ccggtaggga taaactgcaa ttcagatgca tcagggaata
    13141 tgccgcgcac acggatttta acgaagtcgc tgtcttcccc gtagtcatca acccatttct
    13201 gcaactgctg tttgttggtg ccttccaccg tccggctgtc aatctgcgca gttttccagc
    13261 ggtgtttata tttgcggaaa cattcgcgga aacgtccggt attacgcgtc gggtttccga
    13321 acgccaccca gataatctca gtgtcttcgt ccgttagcgc accctcggca acttcccaca
    13381 ccagatccgc aatgttcgac gcttcatcga ataccacgat gatgcgtttg cgctcgttgt
    13441 gtagtccggc gaatgcctca gtgttgtgct cagaccaggg tattgcgtca gcccgccacc
    13501 gcttgtcgtg cccagggtca ttgctgtaca tcgcggtagc ggtacaggta aaccagtctt
    13561 tcgtgatagc aaggttcgac cacttgataa tttccggcca ggtcttcgtt cgtagctggt
    13621 tgtcggtgtt ggcagtcacc acgaccttac aatcctcgca agtggacatg ccccagttga
    13681 tcagcattga gatgaatgct gatttaccaa taccgtgacc cgaagcgcgt gccagcataa
    13741 gcggctgata gcgcgcctct ggattctgca ggtgatcacg tatctctcgg aacgcatcgg
    13801 cctgccactg acgtgggccg gtagcatgtg ccagttcagt cccctcttcc ccccacggga
    13861 acgcatagag ggcatagcca agcggatcgt gagtgaaccc tgcaatatct tcgattaact
    13921 gctcttcagg agataacgct gcatctgtca ctgattacca tcctgacgtt ctttgagtcg
    13981 cttcctggct gctgctatgc gatcagcaat tgtcacattc acattaacat ccagacgttc
    14041 tttgaacgcg ttgacatcaa catgcttacc aatcagctca aggttcttca ccttgtcagg
    14101 ccatttaatt tttttgagga ttgtctctat cgaatcctcg ttcatgttca tgatggtcga
    14161 tgacagatca aagccgctaa gagtagtgcg ccagattttc ggccactcgc gtattggctt
    14221 aaggctccca tcgtcgttga ggatgtcgat cacgtccatc tggtcgatct ccaccaggcg
    14281 catgagaacg taatcagcac tgacgcgcat tcgtttgttg cgctcctcca tcaactcggc
    14341 aatccgtttt tgaatgcgtt catcgcgcat catgacactg gctttaactg ccgctgtatt
    14401 tggggagaat cctgcgttaa tcgctgcctg agtctggttt tcaggcgttt tgatgtatga
    14461 ctggcaataa gcctcctgca ttgctgttag tggcttaaat tgcgttgatt tgcgtttata
    14521 ggttttaggt tcagcaggca tcataaccac cgtggtaata gttaccgttg tggtaatagt
    14581 accatgcaaa ataaagccgc catagttggc ggcagtattc aaagtccatc aaattcatcg
    14641 taaaaactct cgtcaagata cccttcccat ttaccgcgaa tgaaaattac atcctcgccg
    14701 caagggtgct gactgtcgat aactatatcc ctcctggcgc aaccatactt atgcatgaga
    14761 aatttaacct ctttcggaaa atttgctgag ttatctctca tatcttcaag gtcgtagcgt
    14821 atttttggca taacaccttc gtgacatgtc acactattaa tttcgtttca tgccagcctt
    14881 tagtcaccca gcattgcgag tcaccattac acgggcatga atttacagga actctctcgc
    14941 cgcacttacc gcaacgtttt ctgctgatcg attttatacg cccgtgcacg cgtgcatcat
    15001 cctggcggat cagtaacgct atatactcac caaattcgta aggcgcacgc ccggggcgac
    15061 gcgcggcaca gttacgctca agcatttcaa tttcctgagc atcaagcaca atttccagct
    15121 tacgcacacc agatgcagct tgtctggctc tctgagcggc tttgcgctct gctgctgatt
    15181 tagccattct gattttcctg catcatgaga aatacaatca tggcggctcg gagtggatta
    15241 ttcgcatgaa agcaaatgtt ctcggcatta aaaacagctg accactcacc gcgagaatgg
    15301 tggcaggtta gactgatttt attatcaaca ataataggcc atgcatccga tggattattg
    15361 cagtagtcag gtaaagggtt taatggctca aaagttgtat cagtatttcc gtaataccat
    15421 ttgttggtgt tattccctga tgtttccggt ttacacgccc aaaggccttt aaaaattatg
    15481 tctcctacca ttctgttaat ttcaaaatca cttaactgtg aatagtccat cacttcacct
    15541 cctgcggcgg ttctggtaga ggcatccagt gggttacctc tttgagatac aggtcttcgc
    15601 catcaccgtc atcccaagtt ggattgccat cattaaacca gtcgccatat acgccgacct
    15661 gagtgttggg gatgtttggt gggtagttgt ttttaaagtc agctgctaac acatagcatt
    15721 gtcgctctcc catttctggc attcgctcac tacagcttat ccaaccatcc ggagttaccg
    15781 gagagttgcc atttacatcg aagtttggct ctgcgtcctg aaccaggagg atgtaaccat
    15841 tcttggcagt atcaagttct aacgcctcgg tgacggtgcc gaaataacga ttacctaaat
    15901 cagcatcaca agtgcttaca tcaatggaaa cttccatccc ttcgattaat tctggcaagt
    15961 tgtaagtttg gcttacaggc tctgcttcca gcgatgccag agcaatttca taagcccggc
    16021 gctcaatatt gtctcgcacg tccaggctgc cgattcgctc tttgatttct ttaatcagtt
    16081 ctttgtcggt gaaagtggcc atctcactct cctttgatgc gaatgcctgt tgcaatgctg
    16141 tttatgatgc tgtcagtgca tggggtagaa agctgggcat ctccagcaat tctcatgacc
    16201 tcaacatctg catatcgaat accgaggtgt atcagaccgg ctatacctga cttaagccga
    16261 gcattttcca taaacagatc ctttgcccgc tgtttttctg cctcaagctc aacgcgcaac
    16321 ttccctaccg ttagcgcaat atcctcgttc tcctggtcgc ggcgtttgat gtattgctgg
    16381 tttctttccc gttcatccag cagtgccagc acggtagccg gattagcctt ggcaacaaaa
    16441 tcccggactg gcttacaatc aatctccgca atgggttgat acgatgtgta gccatgctgt
    16501 cttgtataac taccgtgacg aataacgaaa aaatcaccat ttattttttt agcctgccac
    16561 ttatcttcac cggctttctc tgccgcttca cgcagtgcct gatagtcaat cttgttcatg
    16621 tcacatcacc ctgaatccgt tgcatttacg taagaaatcg cagatatagc ccttcatttt
    16681 ttcatgccaa tctcgatcat tcccattgca ccaaccatca ggtggagtcc agttttctat
    16741 cagagcagcc attttctttg ctttcgccgg agtagctgtt gcggtatcgc agtaatgacg
    16801 agtgtcaacc aacgcatcca taccatcgat atcaagtacg caaaaccatg tgtgattcgg
    16861 aattcctaca ggtggtattt gttgcccacg tcggcgttta tcaatgagat atacactcac
    16921 tggttgcctc ctctgcgaag tttcgcggca aaatcagccg caacctcttt gaccatattg
    16981 gaaagcagag tgccacctcc gaatttttct gagaacatct ccacgccatg cgcccggact
    17041 tcagccagga aagcgtcggt ggctggcata tttcctgttg ccttcatggc ctccaaaata
    17101 accagaacac catctctccc aatctcctcg cagataacct cggtgctgtc gccaacaaca
    17161 tcgcagaatt cctgaactgc tttacgagcc agctcattct ccgccgccag cgccgaaaac
    17221 ttctcgtgtg ccaacttaac agctgcatca gcctgcttaa ttgactcaat cgctttctgt
    17281 tggtcttcgg ccagcgcatt agcacgcacc agttgcactt ccagttgcgt tgccaaatcg
    17341 ctgatcagct ttgccacact acgcatatca acggcgccac attctgcttt cagttccgaa
    17401 gccatctcat gcccggcggt aactaaccct ttgatattac tttccatctt taccctcgct
    17461 tatccacata acttattgat tacattgata actaaaaaga tcgtcgattc agaactcttc
    17521 gatattccag ccaccacctg ctttctttgg tttaaccgtt accccgatga ttcggaacgg
    17581 atactgatct gcggcgactt tggttttcac cctggcgtcg tcggtccaga aacctttcac
    17641 ttcgtgcagt tccatctctc cggtggcgag catcacagcg aaatcgggcg tataaaacgt
    17701 gttgtcagcc agccgcagct tgataccctc gaaccgatac caggcgactt cccctgcacg
    17761 tttacgctgc tcaaggtgct ggcaatacgc agattctgtt ttgttcatct ggcctgtttt
    17821 gagtcgacca agagcctgta tctgttttct catgatttac ctctaaggta attaaaaacc
    17881 acataagaca cgaaatcaat agatctaaga atattttgtt acctattagg taactattga
    17941 ggcgtaaaaa aatgcgctat cgcgctggta ttacttgata aatcctgctg cctttcctcg
    18001 cctgtattcc tccatcagcc actgcgccgg tgttattccc cccagggtgg cggcgttagg
    18061 catgcacccg aaacttcgcc ctggtggatg gtaaacgtct ctccctgtgt ccggaggcgt
    18121 actcattggt tctggctttg cctgtatgct gatcaccgga tcgggtatct gctgtccgga
    18181 agccaccttt ttcgcccaat catcgagcag cctgcgcgcg tgtttctcaa cctcaatctc
    18241 gctaagctgg cgctgataca ttgcacggcg ggtatcacat acgacccagt acataaccgg
    18301 atgtcgccac gggaatcttt cgggaccacc aggatataaa cttttttcct tgctgtaccg
    18361 gtgaaactcc gccatcacat cgtcaatggt gacgccaaga accatcttgc tgtctttaca
    18421 ccacttgatg aattgccctg gcgacggcca gaacggagat tcactggcgc gggcgtggcg
    18481 cataccagca gaaacctgtt cacgggttcg gatcccccct tcggcaaacg cagcaatcca
    18541 ctgctgtttt gcagcaactt cctgctctgg cgtcttcagg ttggttacca ctgccgccgg
    18601 aaacagttgt ttcagctgtt tgaaaagggc atcaacaagc ctctctgctg acatgttcac
    18661 cacgttgtca ttgttggtgt actgatgctc ataacctgac atgcgagaaa gggcttctcc
    18721 gtcacggttt tgtatcgcgg taaaaacgtt gttcacaaga aatcctccca tgcttcaggg
    18781 ctgttccagt gcggaacgtt gttatcaggt aatgttgatt gcttctgtct gctaatctgc
    18841 agccgccttg ccagcttctg ctcccactgt gcctgatggt atgccttacc ctcagccatc
    18901 cagtaaattc tgaattctgc aagttcctgt gccgttggca gactgtccag gtagatcccc
    18961 tgcaatgagc ttttccgaag aaagtcatct gatggctgcc attgttcatg catgacaaat
    19021 ttgcctaatt gccctggccc accaggaaga acaaagttat tcatcacggc gttgtttgcg
    19081 ccggggtcat gaggcacaga atccccgctt tttgtcctgc tctccctctc ttggttaaat
    19141 gactggttat atgactggtt ctgtatcccg tttttgggat cattcaacat cccgtttttg
    19201 ggatcattca acatcccgtt tttgggatca ttcaacatcc cgtttttggg tatattcccg
    19261 ttttcggtaa cattaccgtt ttcggtaaca ttgccgtttt cgggttcatc acccccttcc
    19321 cggttgcctt taatgttccc gtttttggtt atattaagag agaaaacccg cactcttttc
    19381 gtcgctccct ttctctctcc ggtatctgaa ataagcccca ttttcatgag cgatataagc
    19441 ccggcctgca cggttttttt attcaggcaa gtgtctttaa cgaggcgttc tatgctgggg
    19501 tagcagaggt tatattcatc ggctctgtca gccatcgaga gcagtatgag ctttaatgac
    19561 gagctacctg gatctgtctc ccaggcccaa tctgttgcat gtctgctcat gattaatctc
    19621 ctctatcagc ttgaatgttg tggggaggaa ttaatcatga tctgcttaat ctctgccctg
    19681 atacgacggt ttgattccat ggtgcactca acacagtgtc cgttgtaaac ccagcgttca
    19741 ctgtcatgtc cgtgcttaca tggttttccg gtgtaatagc gtttaagtcc gcgctttgcg
    19801 gcatcaatac gtgtaatgat ttccatggta agccctgtta ttagtattgg gattacggtc
    19861 attttgtgct gacacaaaaa aaagatcaac cagatttggt tttttattac ctttgaggtg
    19921 cgaatagata tgaaaagacc gccggatggc ggtctacagg gggttgtgga tggatatcat
    19981 gagtagaaga agtatgccag ttctgctttt gaacgcagcc attgtcttgt tttacaggct
    20041 ttaaaaagcc cattcatcaa taccttacct ggcatttttc gcttacttgt taagtgagtc
    20101 tggatatagt gactcgtcgt tccggcttcc tgtgcgaagg cttcacgctc atccggagta
    20161 agtgcaagcc agtgcttttt gaaatcgaaa tgtccgttat cgctcatagc tattgcctga
    20221 tatttatttc agataataaa tattcaccca taaggtaaca aaaatcaagg atagttacct
    20281 atggggtgca tttacctgtt gggtaatatt gctttaaatt gaatcatcta ctgattcata
    20341 tatgaggcga ttttccagaa aatgaaaagt atccaggacg tccgcaggca aaatctcaac
    20401 gacttgatcg accgtgaatt caatggtgtt cagacgcgga tggcagaaaa acttggaact
    20461 caggcaaatc tggtaaaccg ctgggctctt ggcaagaagg ttatcggcga ccaggttgcg
    20521 cgaaaaattg aagctgccgc caataaaccc cgtaactggc ttgatatcga tcgctcgctt
    20581 tctcaggaag gttttcagcc tgtcggccca agcgacattg gtcagctagc ggctcacaac
    20641 ctggaacgct ggatgagcga aagccgcgac ctttcaacac agggaaaact tcaccgcgca
    20701 tccggcgtcg cccaggtgac aatcagccgc ctgttaaaca atgaggtcag cgtttccatt
    20761 tccaccctgg agaatgttgc atccgcattc gggcgtcacg gatatgaact actgattcac
    20821 ccgcacgacc ctgcgactat caactatgac cgctcgcgct acgcattgtt acccgaaacc
    20881 gagaaagcaa agatcgaaag ttacattgaa tttgtcatca accagaacga aaaaaacaaa
    20941 caataaaatc atatttttca gtaagtaagc cgccttctgg cggctttttt attgcctgtt
    21001 cgattaccta atgggtaatt tttttaactc atatctattg gcatcaaacc agatacgcat
    21061 aattattacc tcaacggtaa cagaccgagg taacaagtta tgcagtggaa aatcatcaac
    21121 ggttggtact gcgttactgc atgcggattc atgagctgga agttccgcac cttacaggaa
    21181 ggcattaagt gggctttcgt cagcaaagaa gctcgcgatg tggccaacga taacgagata
    21241 tgggagggct gataatgaac gttaatcagc agaaaaatct tcaaaaaatc atgctggcat
    21301 tcgacaagga ctaccgcctg tcagaacagc tatatgaccg acaagttgaa ctgattgaga
    21361 gtatccggct tcatcaactg gcctcaactt tcgacgttgt aacaggcaaa ggcgttcgcc
    21421 aggaagtgct ggaagcagct aaagacagcc ctgagttcga agaactgatg gattcctacc
    21481 ggcgcgaggc aatggcaatt atcgcccgct gggatctggc tgatcagatt gatgggcaga
    21541 gggacgcggc atgaaaccaa cgctcctctc attgctgcga ggtggaaaac acagcatccg
    21601 agatatggca aagattcttg gtatctcaag atcgaaggtt tcttggttca tcgctgagct
    21661 tgaacgtcgc aaatgggtaa aggtaaccag gtgcgcaata tatttccacg atggaacccg
    21721 ttccaacaag cagaacgaat acgaggttaa gttatgaata ctggcatcta tttcgacatc
    21781 agcaacgagg attaccacgc cggtgacggc gtgagcaagt cgcagctaga tatggtggct
    21841 aagaaccctg ccctcctgaa atgggttcag gcagcaccag aagacgaaga gaaaaagtct
    21901 gcactggata tgggaaccgc attgcactgt ctgcttctgg aacctggaga attcgacaaa
    21961 cgcttcatag tttcaccgaa attcgatcgt cggacaaaac aaggtaaagc tgacgaagaa
    22021 gcatttcttc gtgatgtggc ggatatgggg attacggtac ttgatgccga gcagtggcgg
    22081 aaactggagc tgatgcgtga tagcgcaatg gctcacccag cggcacgctg gatgttggaa
    22141 gcacctggtt actgcgaagc atcaatgtac tggaacgatg aagagacggg tgagttgtgc
    22201 cgaattcgtc cagacaaatg gctgaacgag cacaacgtga tcgtcgacgt gaaaaaggtt
    22261 gcagatatgg accgttttgc acgccacatc gaggaattcc gctaccacgt gcaggacgca
    22321 atgtaccgcg aaggcgcaat gaaggttact ggtcagccgc atggtttttt ctttcttgcc
    22381 gtgagcgaaa gcattgattg tggtcggtat ccggtacgcg tgttcgagct ggatgcgccg
    22441 gatgtcgatg ccgggcacgc tctgttccgc cgggatctga atacctatca cgaatgccgc
    22501 atcaacgatg agtggggcgg agtggaaatt attaaacgcc ctgactgggc acgtaaacag
    22561 gatatgtacg tatgagcaat gatatcgcaa tcacatcaca accaggcgca actgtaggta
    22621 ctgctgcggc aatcttcagc ccggagggca tgaatcaact ggtgcgtttc gcggagttga
    22681 tgtcacaaag caaagcgact gtaccgaaac atcttgaagg caaacctgcc gattgtctgg
    22741 cggtgaccat gcaggcggca cagtggggaa tgaacccttt cgccgtggcg caaaaaacgc
    22801 atgtggtaaa cggaacgtta ggctacgaag cacagttggt aaacgcggtc gtatcctctt
    22861 ccagcctgct agcgacacgc ctgaattatc gctggagcgg tgactggtcg aatgttaacg
    22921 gcaaaacaga taaatcaccg aatctgacgg taactgtgtc agcggttctt aaaggagaag
    22981 cagaaccccg tgagcttacc atcagtatgg cgcaagccgg agtgcgtaac tctccattgt
    23041 gggaacagga tccgcgccag caacttgcct atctttgcac gaaacgatgg gctcgcctgc
    23101 acgctcctga tgtacttctc ggtgtttaca cccctgacga attacaggaa acggcaccgc
    23161 gcgttgagcg agacattact ccgcaaacga ccactgctgc gggaatgaac agtctgatca
    23221 acgctaaacc agcgaaaaag cctgatgagc aaacgcgtaa agcggatagc cgtgatccag
    23281 aagaaatgct gatggccttt accagcgcag cgatgaatta cagcactgtc tccgaactgg
    23341 ataaggctta caaatacatt gcacaaaaac tttcagatga tgacgaactg ctggcaaaag
    23401 ccaccgacgt ttacagcgtt cgtcgggaag aattaaacga aacatctatg taaccaccac
    23461 cgcggcgcca cgcgcgccgc actgcaacca agagaggtat ttatgaaagg tgcattaggt
    23521 aagaaggaac tcctggcggt ggtgccactg tcatggagca ctatcgaccg tatggagcgc
    23581 gcaggggaat ttcctaaacg ctggtatatc accgataaac gctgcgcatg gaaccgtgat
    23641 gaagttgagc gttggcttga tgaacgtcag gcagcaagcc cggcagagtt ccagggtaaa
    23701 aaacctcctg ttcagcaacg tgtatatcgt cccgtgagca acgctgcatg agtgcgctgc
    23761 taaggcactg gagcaaatgg tcaggatggt acttattcct ggcctctgtt tcagcatggc
    23821 tttatctgct ggcattaatt ttcaatgcgc agcccttcaa aggttgttgc cgttactcct
    23881 gcggcgatag agatcgaaaa acgcgcgatt gaaagagaga aaaaagggca gttccgcatt
    23941 gccgcccacc tttggcttca gtgtatggat gttgcttctg gtgatgttga gcgtgcaagg
    24001 atcgcggttc gcagggacca atgtatcaca aaaggtaacg gccttcgccg tggcgactat
    24061 agcggcatag gatgttgcgg ggttgtttat gactaagaaa tacacgctaa tctatgcaga
    24121 tccaccctgg gtataccggg acaaagccgc agatggtaat cgcggtgccg gttttaaata
    24181 tccggttatg agtgtgctgg atatctgccg ccttcctgtg tgggatttgg ccgatgaaaa
    24241 ctgtctgttg gccatgtggt gggtgccaac acaaccactc gaagcgctaa aagttgttga
    24301 agcctgggga ttccgtctga tgaccatgaa gggcttcacg tggataaaat gtggtagtcg
    24361 acaaccagat aaactggtta tgggtatggg acacatgact cgcgccaata gtgaagattg
    24421 cctgtttgca gtaaagggaa aactacctcc gcgcattaat gcagggatcg ttcagtcatt
    24481 taccgcaccg cggcttaagc attcaagaaa accagatgtt gttcgtgaaa aacttgtgca
    24541 attgttaggc gatgtttctc gcattgaact gttcgcccgc cagtcgtctc atggcttcga
    24601 tgtttggggt aatcagtgcg aagacccggc agtgcaacta caccctggat acgcgttgga
    24661 tattggcgga ttaacaaatg cattcagcaa tgctccgctg tcaccaacag acaaccaagg
    24721 gcgggagcgt gcagcatgaa cagggcatca ccagcagatt taaggaaatg ccttgaaact
    24781 acaaacatgc ttgcacacag cgggatcagg tttgttccaa ttcccgctgt cactgatgct
    24841 gaatttgcaa cactgtcagc aatattcaca gataaaattg aatcactggc agcagaagcc
    24901 gagatggaag aaaatcagca gaataattaa acgttattcc cccgccatcc acttctcaaa
    24961 cttcgacggg gagaacggaa tcagatccgt atgctccccg tcaatccagg aatcaatcat
    25021 atcggcccac tgctgcaaca tgtaggcgcg ctgtctggcg tattccgctt tgttatatac
    25081 ggcgcgcaca cctttctgct catgtgccag agccttttca atccagtctg aaggataacc
    25141 agcctcatgc aataacgtac tggctgtacg gcgcatatcg tgtacggtga agtcctgaat
    25201 atgctcacca tcttcattta ttattttcac cgttctgtcg atcagagagt tcagcgcggc
    25261 attagataat ggcttccgga agttgtaacg accaggaacc agatattcac ttccaccagc
    25321 gcacatctgc aacccgacca atatatcctg tgcctgttta ggcaggtaga taacatgtgc
    25381 ccggcttccc ttcatgcggt ctgaaggaat tgtccatgtc cattttttaa aatctatttc
    25441 gtcccacgtt gcgttggtga attcgccttt acgaaccata gtgataagca ccagctttaa
    25501 agccattttc atagtgccca tagcaccaat ggcatccagc gtgcggaaga acaggccaat
    25561 ttcttctggt gtcagtgttc gctctcgtgg tttaaatatg gcgatagacg aaggtttaat
    25621 gtcagccgca ggattcaaca aaccatgacc acggtcattg gcgtgacggt atacgctgct
    25681 gatgatctcc cttgcctgta ctgctgttgc ccgaccaccg cgttcgacaa tccggtcaca
    25741 caaatcacga accatcgatg tggtaatttc agtcatcatt ttgttgccaa gaaccggaag
    25801 tatgtcacgg tcgatcaccg cctgcttcat tgcgcgggta ctgtcagcca ggatgacgtg
    25861 tttcatataa ctgtcggtat gtaccgtaaa cgtctcggca ccacgaatct ttttgatacc
    25921 gtcacgttta gccgcagccg gtgactgacc tgctttaagc agcctctttg cagcaatcag
    25981 ttcttctcgc gcttctgcca ggctgatacc gtcacgccca tactgcccga ttaccagtgt
    26041 ttcgcggcga ccgttgatac ggtagtcata gcgaaacgag accgtgcctg acgtaagcac
    26101 agctacatac agcccgtcac gatcggaaac tttgtacagt ttgtcctgtg gcttgaggtt
    26161 ctttaatttt gtatcggtaa gcacaattca cccgtataga aaccattttc atgacggtat
    26221 gagagtatac ctttaaggta ataccgtcac ctgtaccgcc gaaaaatatg gtgtagagtg
    26281 aatagaaatg aatacataaa aacaaaaacc ctctgtaaaa acagagggtt aaattagtat
    26341 ctgaatagga atgagttgct atgagttagc tgttaatcat tcccactcaa tggtagctgg
    26401 cggcttgccg ctgatgtcat acaccacgcg agaaataccg ttcacttcat tgataatgcg
    26461 gttggaaaca cgaccgagga aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc
    26521 gatggtttcg acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac
    26581 gccaacggaa cgtactggca ggaagacggt gaacgcctgg ctgactttgt cgtacaggtc
    26641 cgctttacgc agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc
    26701 tttcttcact tcacccagaa cacgaacgcc aaggcctggt cccgggaacg ggtgacggta
    26761 cagcatgtcg tacggtagac ccagctccag accaatctta cgcacttcgt ctttgaacag
    26821 ctctttcagc ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg
    26881 gtgagatttg atgacgtgtg ctttaccggt tgcagacgcc gcagattcga taacgtcagg
    26941 gtagatggtg ccctgtgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa
    27001 tacttcaacg aaaacgcgcc cgatgatttt acgttttgct tccggatcgt tttcgccagc
    27061 cagcgctgac aggaagcgat cttctgccgg tacgtgaaca atgttaagac caaagtgatc
    27121 gccaaacata tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa
    27181 tacgcaagtc aggtttttac cgatagcgcg gtgcagcagc attgcggtta cggaggaatc
    27241 cacaccacca gagaggccga ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc
    27301 tacagcatcg tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg
    27361 cacaaaacgc tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg
    27421 tacgccatag aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct
    27481 ggctacggtg acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac
    27541 atcgagcagc ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct
    27601 gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg
    27661 accgcccaac tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc
    27721 tgcttcaaag acatactgcg gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc
    27781 gccggaaaga ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca
    27841 cgcccacagt tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta
    27901 ctgagaaccg aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc
    27961 ttattccgag gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag
    28021 aatcaggagc ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg
    28081 ctttcctgaa tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg
    28141 atagtaccac agccggtcag acccatacag gagcgcaggc cgcccatctg ctggtgaatg
    28201 atctctttca ggcgaccttt ataagctacg cgaccttcga taccttccgg caccagtttg
    28261 tcggcagcgt tatcgctctg gaaataacgg tcagaggaac ctttggacat cgcgcccagg
    28321 gaacccatac cacggtaaga tttgtaagaa cggccctggt agagttcgat ttcacccgga
    28381 gattcttcag tacccgccag catggaacct accatcaccg cgcttgcgcc agcggcgata
    28441 gctttggcga tgtcgccgga gaagcgaata ccaccatctg cgataaccgg aataccggtg
    28501 ccttccaggg cttcaactgc gtcagcaacg gcagtgatct gcggaacacc tacgccagta
    28561 acgatacgag tcgtacagat agaaccaggg ccgataccga ctttaaccgc gctgcaacca
    28621 gcttctgcca aagcgcgtgc acctgcagct gttgccacgt tgccgccgat gatttgcaga
    28681 tccggatatt tagcacgggt ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg
    28741 gaggagtcga tcagcagaac gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg
    28801 ttacccgcac ctgcgccaac tgcagcacca acacgcagac ggccttgctc gtctttacag
    28861 gcgttcggtt tacgttccgc tttctggaag tctttcacgg tgatcatgcc gatcaggtgg
    28921 aattcgtcat caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc
    28981 acttcacggg cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg
    29041 ctaaccggct ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc
    29101 agttcgtttt cttcggtaac gaccggatag cccgcaaaac cgttacgctc agtcagttct
    29161 ttcacttcgc gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat
    29221 tcgtgttttt tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg
    29281 ataaagccga taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta
    29341 tccattgctg cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg
    29401 aggtcagcag tattcggcag aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac
    29461 gtcagagctt ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa
    29521 tattgccgcg gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg
    29581 cttgctatcc ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga
    29641 tctcgctcac atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca
    29701 aacggttcgt ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatctc
    29761 taatttcaca caaccggctt ccggtcactg gtactttaca ctcaaagacg acaccgccca
    29821 ggtacgctgc gcgatgttcc gcaacagcaa ccgccgggtg accttccgtc cacagcatgg
    29881 acaacaagtt ttagttcgcg ccaatattac gctctacgag ccgcgcggtg actaccagat
    29941 aatcgttgag agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct
    30001 caaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc
    30061 ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt
    30121 gcatgtgtta aaacgtcgcg atccttctct accggtgatc atctacccca ccgccgttca
    30181 gggcgatgac gcaccggggc aaattgttcg cgccattgaa ctggcgaatc agcgcaatga
    30241 gtgcgacgtg ttgatcgttg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa
    30301 cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg
    30361 gcatgagacg gatgtgacca ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc
    30421 tgccgccgct gaagtagtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac
    30481 ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc aaccgcacgc gtcgctttac
    30541 gcagatccat caccgattac agcaacagca tccgcagctc cggctggcac gccagcaaac
    30601 catgcttgag cgcctgcaaa agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac
    30661 cgggcaacag cagcagcggt taacacagcg gctgaatcag caaaatccac agccgaagat
    30721 tcatcgcgcg caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt
    30781 acagcttagc gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag
    30841 cccactgtca acgctggcgc gtggatacag cgttactact gctactgacg gcaatgtact
    30901 gaaaaaagtg aagcaagtta aagcgggtga aatgctaacc acacgtctgg aagacggctg
    30961 gatagaaagt gaagtaaaaa acatccagcc ggtaaaaaaa tcgcgtaaaa aggtgcatta
    31021 agccagcacg aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta
    31081 atctactgca ccgcaggctt ttaacacctg caacggttga tggcagtctg gacaaagagc
    31141 tttcatttca ataatttccc cgcaggatgg gcaacgcgca tggtcgttat cctgatcaag
    31201 cacatgctga cattgtggac aatgcagttc catatgaatt cctccggtag ccatccctct
    31261 acattcatag agggaatggc agataaaata cttacggata attatttatt tttcttgatg
    31321 tgcttcatca gacgcttacg tttacgcatc tgggttggcg tcagggtgtt acgcttgttc
    31381 gcatacgggt tttccccttc tttgaactga atacgaatcg gcgatcccat tacgtccagc
    31441 gatttgcgga agtagttcat caagtagcgc ttgtaggaat caggcaggtc tttcacctga
    31501 ttaccgtgaa tcaccacaat cggcgggtta taaccaccag cgtgggcata tttcagcttc
    31561 acacgacgac cgcgtaccag cggcggttgg tgatcttcaa cagccatcgt catgatgcgc
    31621 gtcagcatag aggtccccac acgacgggtg gagctgtcat acgcttcacg tactgattca
    31681 aacaagttac caacaccact gccgtgcaag gcagagataa agtgcacacg agcaaaatcg
    31741 ataaagccca gacggaagtc cagcgtttct ttcacctgct ctttcacttc ctggctcagg
    31801 ccatcccact tattcaccac aatgacaagt gagcgcccac tattgagaat aaagcccagc
    31861 agcgagagat cctgatcgga aataccttcg cgcgcatcaa tcactaacat caccacgttg
    31921 gcatcttcaa tggcctgcaa cgttttgatt acggagaatt tctctacagc atcggtgatt
    31981 ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat actcacgtcc atcgcgttcc
    32041 attggaatgt agatactgtc acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct
    32101 tcaccaagaa tacggttagt gagtgtagac ttacctacgt tcggacgacc cacaatcgcc
    32161 agtttgatcg gcagactttg cgggtcgaag tcgtcttcct cttcttcttc gccgttctct
    32221 tccgcttcaa attgcgccca gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga
    32281 tcttccatcc acggcagcag cacatgctcc agcagactta atacgccacg accgtgagac
    32341 gcggcgatcg ggtagatttc acctaaacca agcgagtaga aatcaaccac tgcctgatcg
    32401 ggatccagac cgtcagtttt gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga
    32461 tgtttggcaa tcgcttcatc tgccggcatc aggcccgcgc gcgcatccac cataaacagt
    32521 acgacgtccg cttcttcaat cgccagcagc gactgttccg ccatgcgggt ttctacaccg
    32581 tcttctgtgc catcaatccc gccggtatca atacagataa actcacggcc ttcaatttcc
    32641 gcacgaccgt acttacggtc acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg
    32701 gtgcgagtta gacggttaaa taacgtggat tttcctacgt tagggcgccc gacaagcgcg
    32761 accacaggta ccatgtttaa agcctcattt ttataaatca tcagacaacg cacgctatat
    32821 tcgcgtcgtt gttaaaaaca ggaaaacggc ccctgtccag gagccgtttt caaagtgaac
    32881 gacagagacg attaacgtgt aatagagtac acggttccgt cttttgcctg gatcagcagt
    32941 ttgccgtcag cggcaaccgg ttcagtctgg aaaccggaac tatcaacttt ttgctgggca
    33001 acgaaacgac catcttcgac gttaatccag tgcagataac cttcactgtc acctaccacc
    33061 aggttgccat tatacagcac cggagaagtc agcaggcgat gcaacagatc gctttgtgtc
    33121 cacagcgtaa cgccgccatc aatggtcaac gccatcaccc ggtcattttg atcgaccaga
    33181 tagatgcgat tgccgtcgac gatgaaatca ttcaccgaac ccagttcgcg tttccacata
    33241 atctgaccac tgcgcagatc aagcgccgtc aggttaccat tataggccag cgcgaaaaca
    33301 acgccgttaa caacgacggg agtcgtgtca acatcgctca gacggtcaat ttcggtagaa
    33361 ccggtcgcct gggaaatacg ctgctgccaa atcatctggc cctgttccat cagcactgcg
    33421 ctgacgcgac cattatcgcc acccacgacg gccgcaccaa aagccgttgc cggcgcagac
    33481 tcgccacgca aagagagcga aggcatatcg aggttaactg tccatttgac agcgccgtca
    33541 gcctcgttca gcgcttgtaa ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg
    33601 accaccgggc gcgaaagtgc ttcacccgcg actttagttt gccatgccac agtaccatcg
    33661 ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa tgtagacatg cccaccagac
    33721 acggtcagac cgccagaaag taatgcaggc tctttagaga accagccatc tttctcggcc
    33781 aggctgacag accagatttc tttgccatca tccgcattca gcgcttttac caaaccagcg
    33841 cggtccgctg catagacaac gttgtccgcc agtgccggat gaagattgga atagaagttg
    33901 ccaatgccgc taccaacgga agtgctccac gccgtggtcg gcgtaaactg gttttcaacg
    33961 gttggcaatg gggacatctt taccacatct tcttcgctgt taaacagcga acagccgctt
    34021 aaaagggtaa cggaaagcag tcctggcagc agtaatttac gcaattgcat cgggtccctc
    34081 tcagatggac aaattattaa ttttcatctg catcatttcg ctcagtgccg gagtaacatc
    34141 gcttttcacg cctgcttccc atgcactacg cgcaccttgc ttatcacctt tgctcagcaa
    34201 tgcttcacca cgcaggtcgg caacaatggc agcccaccct tcacctttga tggtatcaag
    34261 ggttttcagc gcggcatcag cctgcttgag ctgtacctga acgcgagcaa gacgcagatt
    34321 tatcacggct ttgagatttt catcgctcgt gtctgccagc ccctgttgta actgggcggc
    34381 agctttctct agttcatttt tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc
    34441 accataagta tttttatttt cagcagcaaa tttttccgcc gccgggatgc tatccggttt
    34501 gccttcgctc actgcggtaa ccgcattttg ataggcaaga gaagcggagc gtgcagaatc
    34561 aacctgatgg ctgttccagt agcgccagcc aatcagtgcg ccaacgccca aaatcacccc
    34621 aacagccagt gctttgccat tttcagcaaa aaagcgttta accgcttcta cctggtcgtt
    34681 ttcgttctcg taaatttcca cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa
    34741 tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac cagagcgcaa atccttcact
    34801 actgctgtgc cgttagccac ttcagactca cccagcacca cagcaacgcg ggcaccccat
    34861 ttatcagcac gggcaaactg tttcttaaag ttgccgccgc cgtggttggt catcaatttc
    34921 acgcccggta attcatcacg cagacgctca gctaatgcca tagccgcaga ttgtgtatca
    34981 gcacctgaag ccaccaggta tatatcgaca acaggatcgg ctttaaattc cggattaacg
    35041 gcctgtacta acaatacaag acgttcgagg cccatcgcaa aaccgacagc cggtgttgca
    35101 cgaccgccca gttgttccac aagaccgtca taacgaccgc ctgcacacac ggtgccctgg
    35161 gagccgagac tgttagtcac ccactcgaaa acggtacggt tatagtaatc cagaccacgc
    35221 accagacgct ggtttacggt gtaagcgatc cccgcgctct caagcagttt gcacagaccg
    35281 gcaaaatgct cacgagattc ctcgtccaga taatcaccta atgccggagc gtcgttgaga
    35341 agcgcctgca cttccggatt ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg
    35401 cgtttgcagt cttcgtccag cttttcttta tgctgctcaa ggaatgccac cagcgcatcg
    35461 cggtaattgg cgcgtgcttc cagcgaaccg atagaattca gctcaagagt tacgtgttcg
    35521 gaaataccca gcgcgcgcca ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc
    35581 ggaccttgca gaccgaaaac ttcgcagccc aactgatgga actgacgata acgccctttc
    35641 tgcggacgct cgtgacggaa catcggcccg atataccaca gacgctgttc ctgattgtac
    35701 agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc
    35761 aggctgtcgc cattgcgatc ctcaaaggtg tacatctctt tttcaaccac gtcggtgact
    35821 tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa tcggcaagcg gatttcactg
    35881 taaccgtagc tgccgagcac gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt
    35941 tcgccaggca ggtaatcgtt catgccgcga atggcttgaa tgttttttgc cacgtttatt
    36001 ctctttctga atataaaaat gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg
    36061 ggttcaatca tacacgggaa gcgaggcgct tcccatcacg ttattatttt tcaacctgct
    36121 gaacgtcaat tcgacgcgct tcgtccagct gactggcttt cgcacgaatg cgtgcttcca
    36181 gctggtcgat catatcgttg ttgtccagac ggtctttgcg cacgccatct tcatagaggc
    36241 cgcttttctt gttgccgccg gtgacgccga gtgtagaaac cagcgcctca cctgggccat
    36301 tcaccacgca gccgataatc gaaacgtcca tcggagtgat gatatcttcc aggcgttgct
    36361 ccagcgcgtt aaccgtaccg ataacatcaa attcctgacg cgaacaggtc gggcaggcga
    36421 tgaagttgat cccgcgcgaa cggatacgca gcgatttcaa aatatcgaaa ccgactttga
    36481 tctcttcgac cggatcggcc gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca
    36541 gcagcagacc taaaccaatg gcggatttta ctgccccgct gcgtgcacca ccggcttcgg
    36601 tgatccccag atgcagcggc tgatcgatct gttttgccag caaacgataa gactcaacag
    36661 cgaggaagac gtcagacgct ttcacgctga ctttgaactg atcgaagttc aggcgatcga
    36721 gatgatcaac atggcgcatg gcggattcca gcaacgcctg cggcgtcggt tcgccatact
    36781 tttcttgcag atctttttcc agcgatccgg cgttaacgcc aatacggatc ggaatgtttt
    36841 tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc attaccgata ttgccagggt
    36901 taatacgcag acaatcgacg ccgtattccg ctactttcag tgcaatgcga tagtcgaagt
    36961 ggatgtcagc caccagcggc acgttaacct gctgtttgat gagtttgaac gcttctgccg
    37021 cgtccatcgt cggtacggag acacggacga tatcagcgcc aacgcgttcc agcgccttga
    37081 tttgattgac cgttgcttcg acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga
    37141 tgggagcacc atcgccaatc ggcacattcc caacgtaaat acgtgttgat tttctacgtt
    37201 gaattggagc ctggttatgc atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg
    37261 tgattgttcg gcattgaggg tcagacgcgc aacctggtta gttctgataa aacgactcag
    37321 atcgacaggt ttcccttgat actggatctg tactgcggct ggcgcaccaa ttttcagttt
    37381 gtatggtgcc tggccggtta agttcaaatt accgtcttta cgctgcatac cgctaaacaa
    37441 ttttttaccg gtagcatcag taacctccag ccagcaatcg gcagtaaagt tcatcaccag
    37501 cgcattcgga tcagccgccg gcgtagtcac gcctgcctga tcggttggca acggcgcagc
    37561 gccatctggc gttgttgttg ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg
    37621 cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct ggtgctggcg cagttacggc
    37681 aggtgtttgc gtgttggttg cggtagtatc cacagacgct ggcggcgtgg acgttgtagc
    37741 cgggtctgta gttgtcgacg tatttaacgg aacactctgc ccctgctcgc tattactgct
    37801 cagttccgcc gaagattgat cggccatagt ggtgatctct tcctgctgag ctttgtggtc
    37861 ttgccaccac caggcaccgc tcaggccgat aaccacaaac aacaccagcc aggtgaaggt
    37921 catcagccag ccgtcacgtt ttttgcggcg tttaccgagg gaaaaactct gcatcggcgc
    37981 aactttcgca gcccgtagtg gagcctgctt ttccagccct ggcagcagtt cttcttctgg
    38041 aatatgtacc agacgcgcat aagagcggat atatccgcgc aggaatgttg aagcaagatc
    38101 ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa accttcaggc aaagtcgctc
    38161 ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt
    38221 agtaagtgct tcattttggt cgtgcgtggc ttcagtattc attcgctgca ggtacgttta
    38281 aatgagaatt gagatgccgg tgaaccatca tgctcaccca caccgcgaaa catccgttaa
    38341 gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac
    38401 taaggctaac ttactgttgc atcgttacat actgccttaa agtcagcaaa aacgcaccgt
    38461 taatattgac cagacaattg caacttaata cttcattcat aaaagattac gccacggtac
    38521 ataaagtaac cgtggcgtaa tggctatcag accgctttaa tgtcgatggc ttcaccctgc
    38581 atccgtttac gcagggtacg tttcgtacgg tcgataacat cgcccgccaa ctgaccacag
    38641 gcagcatcga tatcatcacc acgggtttta cgcacaatag tggtgaaacc gtagctcatc
    38701 agcacttttg agaaacggtc gatacggctg ttcgagctgc gtccatacgg cgcgcccggg
    38761 aacgggttcc acgggatcag gttgatctta cacggcgtat ctttcagcag ttccgccagt
    38821 tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca tcacatactc aatagtgaca
    38881 cggccctgat tggcgttgga tttctccaga taacggcgca ccgcagcaag gaacgtttcg
    38941 atattgtact ttttgttgat cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc
    39001 agggaaattg ccagtgcaac gtcgatcata tcgcccagtt tatccagcgc cggaactaca
    39061 ccggaagtag aaagcgtgac gcgacgttta gacaggccaa aaccgaaatc atcaagcatg
    39121 atttccatcg ccggaacgac gttgttcagg ttgagcagcg gctcgcccat gcccatcatc
    39181 actacgttag tgattgggcg ctgaccggtg acttttgctg cgccgacgat tttcgccgca
    39241 cgccacacct ggccaataat ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg
    39301 gtggaacaga atttacactc cagcgcacac cccacctgcg aagagacgca gagcgtggca
    39361 cggtcgtctt ccgggatata caccgtttcg acgcgctgat cgcctacggc gatcgcccat
    39421 ttaatggtgc cgtcggatga acgctgttct tcaaccactt ccggtgcgcg gatttccgcc
    39481 acctctttca gtttgccgcg caacactttg ttgatgtcgg tcatctcatc aaagttgtcg
    39541 cagcaatagt gatacatcca cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa
    39601 tctttaaaaa actcccgcat ctgctgacgg ttgagatcca gcaggttgat ttttccatct
    39661 ttcgtggtga cgttttcagg tgtgactaat tgttcagaca tatgctattc cggcctcgtt
    39721 attacacgtt atggcccctg gagggttgaa aaaagaaacg ccccggtgag cggctgctcg
    39781 tccgggggcg ctgcattgta caaattctgg cgcacggatg ccacgtttgc acgcggcatt
    39841 tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc gccaaagaaa taagcgattt
    39901 cgcgagcggc agattcgacg gaatcagaac cgtgggtacc gttttcggtc aggctgtcag
    39961 cgtaatcagc gcgcagagta ccagccagtg cgtttgccgg attggtcgcg cccagcagat
    40021 cgcggtgacg ctgaacggcg ttttcacctt ccagcacgga aaccacgatc gggccagagg
    40081 tcatgaattc aaccagacca tcaaagaacg gttttccatc gtgttcagca taaaagccac
    40141 gtgcctgttc aacggtcagg tgcagcattt tggtgccgac aattttgaac cctgcagctt
    40201 caaagcgcgc aaagatatta ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg
    40261 aaaaagtacg ttcaatagcc atttttacct ctgtaaattg ttctgttgtt gtctgtacca
    40321 gcgtacgaga tggcgcggat tataatgagc aacagggccg ttgactattg atgaaggtaa
    40381 cattttttta aaataaaatg agttttagca acaatcagga gcaaaacaga ctattgcatg
    40441 acaaatttca ctgtcgcgat ttgccccaca tcatccatca ccagcaattg ataatcacct
    40501 ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga
    40561 aaccaccagc gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg
    40621 cccggtaaac gtttaataat cgcgccatcg cgaacgcctg tcagctgcag agggagcttg
    40681 gcatcgtgac cgtatggcgg gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt
    40741 tctgatgcgg gcagccaggg ttccagcggt agcggccaga catttatcat ttcttgtcgc
    40801 gcttgcgggc aatcagcggc aacacgtttg ccattttcat ccagccagat ggggaaacga
    40861 atgccattaa tgccttcctg ctccggcagt aatagagttg gcggctgact cccgtccagc
    40921 agccaggttg ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc
    40981 ggccagcaga taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattcgca
    41041 ctgcgcgaca gtaagatatt attgacctga ttcaacaatg gtacggcact ggcaaagcca
    41101 aactgaccaa caacgggcgt gccgtccggt ctgccagtcc agatcccaat gacatagcga
    41161 gcgttaaccc caatcgccca ggcgtcacga tagccatagc tggtgcccgt tttccatgcc
    41221 agtggggcga cgcgcggcaa ggcactatcc ggcaagggtt tcgcttcatc agccataatc
    41281 cggcgaatga tccacgccgc ccccgacgac attaaaggcc gttcaagcag cggatcgtca
    41341 ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa acgcggtata cgctgccgcc
    41401 atatcttcca gttttgcacc agcgccgccg agaatgagtg aaagattcgg cgcagcaccg
    41461 ttgggcaaat ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca
    41521 taggcttcca gcacctgcac agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg
    41581 ctgatcgggc catgaaaacc gctatcaaag ttacctggtc gataatcacc ggtgcgccgg
    41641 gggacgtctt gcagcagtga tgccgggtgg atcaagcctt catccagcgc cagaccataa
    41701 acaaacggtt tgagcaccga tcccggcgat cggatcgcat tgaccatatc aacatgacca
    41761 aagcgtgaat catcgttgag atcaaccgat cccacccagc cgcgaacacg catatcggta
    41821 tgatcaacca cgatcatcgc cagtgagctg cgcggtggca accgcccttt ccagttttgc
    41881 gccagttctt ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt
    41941 ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt
    42001 gccagccaga tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc
    42061 acggccatcc gttcgagcac tttattacgc gcggcttcgg cacgctccgg ccaacgatcc
    42121 gggcgaagac ggctgggcgc ttgcggcaaa accgccagca ttgccgcctc ggaatagctt
    42181 aaattcgcag gcgactttcc gagataagcc caacttgccg caccgatccc ctgcaacgta
    42241 ccgccaaacg gagcgcggtt aagatacaag gtcagaattt cacgcttaga cagatgccat
    42301 tccagttgca acgcgcgcca gagctggcga attttgccgc caaatgtttt ggggtgagga
    42361 tcaagcagac gagcaacctg catagtgagc gtgctgccac cggaaataac ccgtcccgaa
    42421 gtgagatctt gccatgctgc gcgcgccacc gagaatggat tcacccccgg atgcttccag
    42481 aaccagcgat cttcataatt gatcagcgct tcaaggtaac gtggagaaac atcttcgatt
    42541 gttaccggat aacgccagat gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc
    42601 tgcgccacga ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct
    42661 gctccccacg ctgcaagaaa aaggagaaat ggcgcagctg ccagcgttat ccagcagccg
    42721 cgtttggtta acaagcgagg catttacggt cggacaatca gcagatcttc agccgcgccg
    42781 gtcgcccgcc attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt
    42841 cccggcgtca ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca
    42901 accgccgcca caaagcgatc gtcacggaac tcaatgtgct taatgctcgc ctgctgcatc
    42961 tggttcagta agttttgcac ttcgccacca ctttgctcca ggctggcgct accgttcgcc
    43021 agattctgat tttccagttc cagacccgca ggcagcagat ccacgactaa cgcatccggc
    43081 acgctgttac tggcttttac ctgcaaccag accagcacca gatcgccgct acgtaacgag
    43141 tccagcgatt tgctcttacc atcagtacca agaatatgac gctcgatttg cagcacattg
    43201 ttcgccggta aaggtgcgga ttgcggataa ccgctggcat ccatacgcaa ccataacggc
    43261 tgatcgccac tgtttctcac ctgcaaggtg gcaagttgat cgctattcag attgctgttt
    43321 agcgttttct cgcctgtcag cggctcagct gagaaagagg tttgcgcctg ccatttaccc
    43381 ggtaaatcct gaatcgtacg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc
    43441 cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt
    43501 agcagtttat tttcttccag caaggagagc attaacgcgt tgtcgcgcag tggactaccg
    43561 taatcaccca gccatatccg ctcatcacta tttctcggcg ttttcagcgc cagcacaatc
    43621 gcttcttcac cacgcgttgc atcacccatg gttttcagcg caacgccaag ttgcagcagc
    43681 ggtaaaccag aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagtggt
    43741 gctttttgct gacgggccag caccagcgca gcgtaagact gaacggcgaa tttactggct
    43801 ttgagattat ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga
    43861 cgctcattac cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg
    43921 accaggaaat ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac
    43981 agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc
    44041 gatgcacggc gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg
    44101 ttggtataaa gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac
    44161 gcttttaact ctttgatata acgtgcaatg ttcagcggtg gtttgccgct caacaacaat
    44221 tgcccttcca gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc
    44281 tcaccaggct gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg
    44341 ccgattttcc actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta
    44401 atggtggcct gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc
    44461 gtagtacgca cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc
    44521 aaaccactgg cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata
    44581 tccagcgtca gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca
    44641 atcaccggtg cggcaactat cactttgctt tcgttgctac cgaaatcatc tgccgtccag
    44701 gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct
    44761 tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg
    44821 accggcggtt taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc
    44881 agacgcccct gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt
    44941 ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca
    45001 ccactatcaa cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg
    45061 ctggctttaa ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc
    45121 gccagatcga ggcgacggtt ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt
    45181 tttggcgtcg cggagcgaga tttatcgcca ggacgtacca ccagcgtact taaatacaga
    45241 tcatgacgat tccaggtttt atcgaccgga atcgtcagat ccagcccttg agcccgaaca
    45301 tcaatctctt gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc
    45361 gccgttggcg cggcgatatg caacttaatg gtgtcgccag ggcgataact ggctttatcc
    45421 agtttcagcg tgacacggtc gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag
    45481 ctatagccag cccagaaacg aacactactg accgcttcat tcggcgcttt gacttccaga
    45541 cgataagcgc cccactctac cggaaaactg accttgccgg tttcgtccgc tttcagatcc
    45601 agagtttgtt cattttcgat cagatctttt tgatcaaact gtgactgcca gccttcatct
    45661 tctgaccagt tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac
    45721 acggcttttt tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct
    45781 tcatcaacaa tgggctgttt caccgtgctg tcagtacgat aatcgtaaac cgatttcgag
    45841 gcgaactgcg gacggatccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg
    45901 cgcgtcaccg ggcgaccgcc cgattccagc aaactaccct ggaaaataac ctgtaatggg
    45961 gaatgcgttt ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc
    46021 gtcaactgaa cttcatccag cgtgcgggaa agattttcgg cagctatatc gccgaattca
    46081 aaaccaggta aggctgacac agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa
    46141 gtattaccat tagcaggtgc accatacagg tagtacccca ccacggagaa tttcacttca
    46201 tctttcggcg ttagcggggt tttctcaccg gtcagattca gcgccatgcg ctctggcata
    46261 aaatcttcga cgtggaaatc ccacatccga tactggttat cgcccgtgtt agcgcgaata
    46321 tgccacatac cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattc
    46381 tccggctgac taacgacgct cctgagtacc tgcccatcgg gtttaatcac gtctaacttg
    46441 atgggttgat tgggcaacgc tttaccgtct gcatcacgca gcaaaccatt gaggattacc
    46501 gtttcacctg ggcgataaag atcgcgtggg ccaaacatga aaaactgttt gctatagcct
    46561 ggcgcgccag caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc
    46621 gttgtctgac cgtctttacg cgccaacagt aatgccgcgt ttttatcatt ttccagctgc
    46681 acatgcccct gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa
    46741 gagacttcaa ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga
    46801 cgattgtgat aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga
    46861 ttactgtaat cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga
    46921 agcggtttga tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg
    46981 agatcaaacc gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag
    47041 ttcgccagcg aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta
    47101 acgcggaaga agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca
    47161 acgactttgc caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg
    47221 cgggtagtta tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc
    47281 tccttgccaa tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgtaactct
    47341 ttaagattat ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca
    47401 tgaataacgc gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc
    47461 gtggctgcac catcaagttg gacttcagag agatccagca atgtcagcac cttaccggca
    47521 ctctgttggg ccagtttttg tctctccggc acggagagtt ttgctgaact cgcgttttct
    47581 gaagaggccg ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta
    47641 tcgttgttgt cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt
    47701 ttcatttttc atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga
    47761 gaatttacgt tatgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt
    47821 ggctcagaca gccaaatcag cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt
    47881 tagcctaacc ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga
    47941 ctggctcgcc gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc
    48001 gcctggacag gaagatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc
    48061 agtgtttttt gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc
    48121 gcgcccggaa acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct
    48181 gattgtctat gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac
    48241 ctttggtgta gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga
    48301 tctgctgtta gaagaaggtg cagtagagct gccggaagga gagtttaacg ccgcgtttaa
    48361 tcctgaagcc gtggtgaaag taaccgatgt attattggca agccatgaaa atacggcgca
    48421 aattattgat gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg
    48481 tttacgtcgc ggacatattc ccggagcact gaatgttccg tggacggaac tggtgcgcga
    48541 aggcgaacta aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta
    48601 cgacaaacca attatcgtca gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc
    48661 actcgcgacg ctggatgtgc caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg
    48721 cgcgcgggca gatttaccgg ttgagccagt gaaataagta ttttacaggc aataaaaaac
    48781 cgccgaattt ggcggttttt tattgctggt ctggttcgcg gcctttccag caggttgtat
    48841 taccgtagta atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg
    48901 tcaagcaaaa gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc
    48961 cgtctcgcaa gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc
    49021 gtctcagcgc ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc
    49081 atggaccgca caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg
    49141 ttttcccaag taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac
    49201 cttcagagca gcaagtaagc ccgaatgccg ccctttaggg cggcatattt tagattatcc
    49261 ggttttgttt aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcacaatat
    49321 gttcggtaat aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg
    49381 gttcatcgcc aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga
    49441 tatcaccatc ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa
    49501 ttgaacaaat aaaagcacgc ttcaccgact taatggtttt aaataaggtg gtgagtgaat
    49561 caatcatttg tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca
    49621 ggatttcctg gctgctcagc ggatttccct cttcaccaat caacaaactg atttcacgcg
    49681 gcataaattc tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca
    49741 tctcaaacaa tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt
    49801 gaagcgcttc taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt
    49861 gctgtaaatc aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca
    49921 cccagacggt ggattccagt agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg
    49981 ctgctttttc cagcaggtct tcaagttcgt tttttgtttc ggacatatga accacaattc
    50041 attcaacgtt cggggcgcaa atgcctgatg cgctacgctt atcaggccta caagggattc
    50101 gcaatttgtt gaatttgcag aatttgtagg ccggataagg cgtttacgcc gcatccggca
    50161 tattagttta cgccgttaac agattagcta tcgtgcgcac accaagtccc gtagcgcccg
    50221 cagaccactg ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc
    50281 cttgctgata gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg
    50341 gatacgccgc gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt
    50401 ggaattccgc cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagca
    50461 aacgaccggc aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag
    50521 ttttcgccgc cccggtgagg gtcgccgcat caatgatcat ttccggtttc tgcgcactgg
    50581 catcaatcag accatcggca agcaccagac gcccttcggc atcagtgttc atcacttcaa
    50641 cttttttacc gttgcgatag gtgatgatat cgcccagctt gaacgcattg ccgctaatca
    50701 ggttatccgc acagcagagg aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa
    50761 atgccagcgc cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa
    50821 acgcagtctg tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc
    50881 acgcgtacac cggtgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg
    50941 gagaacgttc tgaaccgcgc ccgacggtgt gcagccccat ataaccttgc tcacgcagat
    51001 cttcgccttt ggtgatccga taagtcacac gatcgcccgc gacgttgctg atcagatcaa
    51061 cagcacgctg tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac
    51121 gcacccagtc gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat
    51181 ccggccactc cactttacgc gtgcctttcg gggctttgta accttgccag aatgcccagc
    51241 agcgatccgc atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa
    51301 tcttgcgcgc cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa
    51361 tgccgtcatt attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg
    51421 tagagagggt aatcttcatc gcttctgtca ttttagttat ccttctttgt aaaagggccg
    51481 ccagttggca gccccaggat ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt
    51541 gagtgaagcc ccaggagcat agataactat gtgactgggg tgaacgagcg cagccaacgc
    51601 atctgcggcg tgaagcatga cgcggaaatt attcggcctc gtccagccag actaacaaaa
    51661 tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc
    51721 aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat
    51781 cgtacagtgc ttcgccaatt tcgcggctat cggtccattt aagtcccata ctaacctctg
    51841 ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc
    51901 ggtgacgcgc gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag
    51961 catgtcgtct tcctgctctg agctttccgg cagcgagtca aaaccttcac gaacgatgca
    52021 gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg
    52081 cagagctgcg tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca
    52141 gagatcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc
    52201 ctttcagcgc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt
    52261 tgtctacgtt tttaatcgct tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac
    52321 tcaggtgagc ggcagcatcg tcaatgacct gacgttctgc ggcgcttaac agcgcggcat
    52381 cagcagccag cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg
    52441 ccagcattcg ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag
    52501 cgatttcgct atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg
    52561 tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc
    52621 gaatatgcgc accaccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg
    52681 agcggcagtc ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct
    52741 gaccatcttt aaaggtggtg aaatcctgag cgcgggccac cggaatcgtg gtattacgcg
    52801 ggatcacttt ctccaccagg ccgcccatcg tttcgaggcc cagcgacagc gggatcacat
    52861 caagcagcag catttcgctg tccggcttgt tacccaccag aatatccgcc tgaatcgccg
    52921 cgccaatagc gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt
    52981 cgcctacccg ttcacgcacc agcggcacac gagtagaacc gccaaccatc accacttcca
    53041 gcacttcatc agcttctacg cccgcgtctt tcagcgcacg acgacaagcc agtaaggttc
    53101 gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc
    53161 ccgcaacgtt aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggcgg
    53221 catccagcag ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc
    53281 gaatgtaatc cgccagcaga tgatcgaaat catcgccgcc gagcgcggaa tcaccgccgg
    53341 ttgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc
    53401 caccgaggtc ataaacggcg atcacgcctt cctgaccgga atccagcccg taggcgatag
    53461 ccgcagcggt cggttcgtta agtaagcgca ggacgtgaag gcccgccaga cgcgccgcgt
    53521 ctttggtgcc ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat
    53581 ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag
    53641 aaacgcgcac cgggttcagc agccccgccg ccgtttcaat catcggtagg ccgttttcgc
    53701 tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc
    53761 ccatcaggcg tttaacagaa ctaatggtgt tggcggtatc gagcgctgcg ttagtacgcg
    53821 cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac
    53881 ggccttcatg gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt
    53941 tggttgtgcc caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac
    54001 caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt
    54061 ttttcttcga gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacg
    54121 gtatccgccg ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg
    54181 gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc
    54241 tgttcgatct cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg
    54301 cgcacagtat gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg
    54361 cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg
    54421 gcggcgagtt gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt
    54481 agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca
    54541 aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat
    54601 caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa
    54661 cgtggaagct ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc
    54721 cttcattcag gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt
    54781 tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt
    54841 cgtcaacaaa ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac
    54901 gcaggccaaa ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt
    54961 cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta
    55021 tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc
    55081 ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga
    55141 gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcaga accgcagccg
    55201 taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc
    55261 atcacgtcgc cacaggccgg tgcccccacc atgccgctgc cgacgttctc gtcgttgttg
    55321 tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc
    55381 attataaatt ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga
    55441 tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat
    55501 ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag
    55561 aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac
    55621 ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc
    55681 ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg
    55741 tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg
    55801 ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag
    55861 gcctcgccca ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg
    55921 tgaccgccgc cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc
    55981 gcaccgatac ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc
    56041 aactggctca ggtcgatagg cagtttaccc acgctctggg ttgcatcaac gtgatagata
    56101 atgccacgag cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg
    56161 ttatttacgt gcatgatgga aacaaggatg gtgtcgtcac gcatcgctgc ttcaagttct
    56221 ttcaggtcga taatgccgtt acgctgcggt gccaggtagg tgacttcaaa accttcgcgc
    56281 tccagctgac ggcaggtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc
    56341 ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt
    56401 gcaccagagg taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta
    56461 cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg
    56521 ttaccaaagg ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc
    56581 accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg
    56641 tacatcactc aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt
    56701 atttagcact ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc
    56761 gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc
    56821 acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg
    56881 tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct
    56941 ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg
    57001 gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa
    57061 accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc
    57121 tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca
    57181 agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca
    57241 aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa
    57301 gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta
    57361 accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca
    57421 ccttatccga cctacggttc tgtacattgt aggcctgata agacgcgtta agcgtcgcat
    57481 caggcaacta ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa
    57541 tcccgcgcag gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca
    57601 atttattcat cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt
    57661 gttccagatg accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat
    57721 gctcgacctg ctcgccgttt tcctgagttg ccagccaggc catgcgcact tcataggcaa
    57781 taacctgaac cgccatcgcc aggttcagcg agctgtattc cgggttagcc gcaatcgcga
    57841 catgataatg gcatttctgc aactcttcat tggtcaagcc gacgcgctcg cgaccaaaca
    57901 ccagcgccac cggggtattt gccgcttcag cgacgctttt caggccgcat tcgcgcgggt
    57961 cgagcatcgg ccatggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac
    58021 cggctaacgc ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc
    58081 ctgctgccag ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat
    58141 tggttaatcc cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg
    58201 tctccaccag cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc
    58261 acgcatctta tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt
    58321 tcccgttctt taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc
    58381 gcgcagcgcg caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag
    58441 aagcgagcca gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg
    58501 tgattatcga cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg
    58561 gtgaacttga aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta
    58621 ccaactttat caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc
    58681 gcaccgaagt tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg
    58741 gtcagggcgc acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg
    58801 gtaccattct ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca
    58861 acatcgtcgg caaactgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc
    58921 tggatctggc ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc
    58981 cgtgggattt cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact
    59041 tcaccggtgg tcataactat atgctgaccg gtaacatcgt tgctggtaac ccacgtgttg
    59101 tgaaagccat gctggcgaac atgcgtgacg agttaagcga cgctctgaag cgttaatgac
    59161 tcaggcgggt gatgtcactc acccgccctc gcctttcagg cgctattctg aaatacttcc
    59221 tcaccgcttt actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa
    59281 agaagtcgca aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt
    59341 ttattctctt tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct
    59401 tctattatcc tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat
    59461 ttacggctaa ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg
    59521 ctgacaacgc catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc
    59581 actggccgct ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc
    59641 gcgggtttgg taaatcaaaa ggcacgccgt cccaagccgg attgctggac gatacgcaaa
    59701 gtgccatcaa tgtggtgcgc catcgcagtg atgtaaaccc acaacgtctg gtgctgttcg
    59761 ggcagagtat tggcggggcg aatattctgg atgttattgg tcggggtgat cgtgaaggca
    59821 tacgtgcggt gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga
    59881 tccccggcag tggctactta cttgatgaga gttacagcgg cgaaaattat atcgccagcg
    59941 tcagcccgat cccgctttta ctcattcacg gtaaagctga tcacgttatc ccatggcagc
    60001 acagcgaaaa gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg
    60061 gcgaacacat tgatgctttt tccgatcgtc acggcgatgt ttatcgcgaa cagatggtgg
    60121 actttatcct tagcgcgttg aatccacaga actaacccat gatcgctagc acgataatca
    60181 ttcacaaaac caccttaaga catgctaatc cactggtcag aacagtttaa gatgagaaaa
    60241 attctgtgac gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc
    60301 aaactttagc tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt
    60361 gctccaccat ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt
    60421 cagccggggt tgaccgccac cacggcaact ttcagcgagc ttaatggtgt ggatgatgat
    60481 attgccagtc ttgatatcag cgaaacagga caggagatcc tgcgctatca tcaactcaca
    60541 ctgacggctg gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg
    60601 tgtttatttc actggctacg acgtggtttc cgtctgtgtc catcatttat taccagccat
    60661 ttcacccccg ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac
    60721 gataccaatc tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa
    60781 acgcgcgata ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca acaccacgct
    60841 ggaattacgc ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt
    60901 caggtagcgc aggaaattgg acgccactgg cagcgtcggg cgctccagcc tgtaccgcct
    60961 gatgagccac tgtttatggc attacttttt tccatgttgc gggttcccga tccattgcgt
    61021 gatgcgcatc agcgagacag acaattgcgt cagtctatca aacgtctggt aaaccatttt
    61081 cgtgagctgg gaaatgttcg tttttatgat gaacaggggt tatgcgatca gctttatacc
    61141 cacctcgccc aggcgttaaa tcgcagtttt tttgccatcg gtattgataa taccctgccg
    61201 gaagagttcg ccagactgta cccacgcctg gtgcgcacca cccgcgcggc gctggccgga
    61261 tttgaaagtg aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt
    61321 ttcggtgcct ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc
    61381 gggaatgata gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg
    61441 ccgctcaaca ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc
    61501 gcggcactga ttattgcgcc ttataccatg ccattaccgc tcttttcacc accgctgatc
    61561 tatacggacc tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca
    61621 gcatgaggga acaactttcg ggcgcaaaaa cattgccggc agcgccacca gcgccattac
    61681 ccagaacacg ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc
    61741 gatactgccg cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctacc
    61801 ctggcgagca gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg
    61861 cagaatttgc accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg
    61921 cactacgccg caaatcgccg agatcaacag catatcgcgt gcactacaac ggcggaaaag
    61981 tttattactc agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc
    62041 caccgccgag gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc
    62101 cgcatgtgcc ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag
    62161 cgccaaccac gctgaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat
    62221 cgtcggacgg atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcgcgag
    62281 gatcacccga taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa
    62341 cgccaccgag ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt
    62401 attcgccagt gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat
    62461 caccagcatc agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt
    62521 cagcagtgcc agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc
    62581 gatgagcaaa ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt
    62641 ttctggcgtt aaaccaatcc ctttaagcca gacgctccag aaaggtagaa aaatgccgta
    62701 actaaaaaag tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag
    62761 tccctcccgt caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat
    62821 aagaatattc gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact
    62881 gcgatccgcg acgcatgttg caaaaataat gcgtaattat gccgttacgt ttgccaaacg
    62941 ttcctgcaca atggcgatga aatcgcgtaa tgccggcttc atctcccctt tcttccacgc
    63001 catcagtaaa gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgtccggt
    63061 attaaaatta ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt
    63121 catggtcacc agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc
    63181 aaaccacgct ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa
    63241 attcacgcca tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac
    63301 cggtaacaca accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata
    63361 aacaggatgg cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg
    63421 cgtggtgatt aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg
    63481 taatactttt ggcagtaaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct
    63541 gtcttcctga acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc
    63601 cagcgcgtcc tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct
    63661 caccagtaac ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg
    63721 ctgtgaggta tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac
    63781 gaaatagcgt aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag
    63841 ctggtagaaa ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa
    63901 aaatagaagg tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat
    63961 ttgaacattt accaactgat tgatacccaa aatggtcggg tcactccgcg tatttatacc
    64021 gacccagata tttaccaact ggagcttgaa cgtattttcg gccgttgctg gctatttctc
    64081 gcccacgaaa gccagatccc aaaacctggt gatttcttta acacctacat gggggaagat
    64141 gcggttgtcg tagtgcgtca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc
    64201 caccgggcca tgcgtgtgag ttatgcagat tgcggcaaca ctcgcgcctt tacctgcccg
    64261 tatcacggct ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc
    64321 gcctacccac aagggttgtg taaatcccac tggggactaa acgaagttcc ttgtgtggag
    64381 agttataaag ggctgatttt tggcaactgg gataccagcg caccgggcct gcgtgattac
    64441 ctgggtgaca ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa
    64501 attgtcggcg gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag
    64561 ttcgccagtg accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg
    64621 gcgaaagatg atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg
    64681 gaaaccgcca aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt
    64741 actgaaaaac cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa
    64801 acctatgccg aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat
    64861 aacaatatat tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat
    64921 ccgcgcggcc ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc
    64981 gatgaagtta aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt
    65041 ctcgagcagg atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc
    65101 gcccgcaaca gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac
    65161 ggcattcctg gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa
    65221 cgctgggccg atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgctgct
    65281 taccagcagg aggtgatgaa atgagtgcgc aagtttcact agagttacat caccgcatta
    65341 gccagtttct ctttcacgaa gccagcttac tggacgactg gaaatttcgt gactggctgg
    65401 cgcagctcga cgaagagatt cgttacacca tgcgcaccac agttaacgcg caaacacgcg
    65461 accgccgcaa aggcgtccag ccaccgacaa cctggatttt taatgacacc aaagaccagc
    65521 tggagcggcg aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac
    65581 gcacccgtca cttaatcagc aactgccaga taagcgaaac cgacatccca aacgtatttg
    65641 ctgtgcgggt aaattatctg ctttatcggg cacaaaaaga gcgcgatgaa acattctatg
    65701 ttggaacgcg tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac
    65761 gggatatcgt cctggatcaa gcggtaatca cttcccataa cctgagtgta ctgttctgat
    65821 gaatcgaatt tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggatcga
    65881 tacctcgccc gtcatcgccc tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg
    65941 ttgcagccat ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga
    66001 gtgcccgcta cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc
    66061 caccgatccg ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt
    66121 accggaggcg cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga
    66181 tcgggattag ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca ggttgccacg
    66241 ctggaactgt cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg
    66301 gcggtggaag gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg
    66361 actcgttccg gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc
    66421 tcactggtta atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc
    66481 aacgtactcg gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa
    66541 ggcagcatga ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg
    66601 tacaccgcca gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca
    66661 ccgaaagtgc gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca
    66721 caggcgctcg ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc
    66781 gccattttac cgctgcaatt tttcccgcaa ccggcggatt ttacgggtcc gtatgtgatg
    66841 ttggcatcgc ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt
    66901 ttagcgattc gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg
    66961 aaagaaaaaa ccatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg
    67021 ctacgccagc aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct
    67081 tatgaacgac ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag
    67141 gtgttacccg ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc
    67201 aaaacgctgg gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg
    67261 gatcagcttt ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg
    67321 ggagaacgct gctttaccct acgccatgcc ggtgatgccg ccagactgcg agaagttctg
    67381 cagcccgaac ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc
    67441 agcgccacgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac cgtcatgggc
    67501 cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccaccagca ggctggtgtg
    67561 cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt agaactgacg
    67621 ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg tatcagcgcc
    67681 aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt cattgatgag
    67741 gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat cactcgtctt
    67801 gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt
    67861 gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg gttctggagc
    67921 gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga ctggctttgt
    67981 cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc
    68041 ggtgcggtca cgctgaatca ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc
    68101 ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa atcactgtaa
    68161 ccaggataat tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag agtgtgtatt
    68221 acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg ttattttgat
    68281 tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt cttaattttc
    68341 attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata tatggcctcg
    68401 ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga agtgcccgcc
    68461 gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt tattttctac
    68521 acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga tgcggttggg
    68581 ccaaatctga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt gctattggca
    68641 attaccgggc cgggggcaat ttctctcgat cggcgttagg aaagatgccg gatgcggcgt
    68701 gaacgcctta tccggcattt tataaattac agccactcta cccgcaacga catctctgag
    68761 gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg atgatgatca
    68821 tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc tttatcaaac
    68881 gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc catgatgatg
    68941 gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc aaaaccattg
    69001 ttcacccact ggcgcggcaa cggcgcgagc tggttaaaat ccagttcctg cggtagctgc
    69061 tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct cgcctgtgcc
    69121 tgaatccgcg tttgcggcga caacggaaaa taaggatgcc agccggtacc aaatggcagc
    69181 gtctctgccc cttgattggt gacagagagc gtcaccatca gcgtatccgc cgttaaatga
    69241 aacgcctgac ttacccgata gtgatagaca ccgctgcgat gttcatacac cagacacaaa
    69301 ctatcatcgc tgtgcgagac acattgccac tcgcccagcc agccatcgcc gtgtagatag
    69361 tgcgcatccc actcaacatt cggttgcagt tgatactcac gcccctgcca gacaaaacga
    69421 ttgccgctca cccggtttgc aaatggcacc agcggaaaac atgaggcatc ggttgccaca
    69481 ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc ttcgattacg
    69541 ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata gatggtcatc
    69601 cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg ctcgccatca
    69661 gcgcataggc atcacctgcc tgttccagcg agaagcgatg ggtaatggcg ttacgcggcc
    69721 acagcttcca gtccgtcaga tcatgggcgc atttttccat atggaacaga ctggtcaccc
    69781 aggagccgat aatccgccgt tgatggtgca tcagatcggc gctgacctcg aattccactt
    69841 ttccggtttc accaatgtaa accacccgtc cccagtcagc agtggattgc agtgccagca
    69901 agcgacctgc ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg tgggtaagtt
    69961 cagcaattat ctgcggcaga ccttcggtgg tcgctaaata gccgtgatcc atcaccccta
    70021 actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc cgttttgcac
    70081 cgcgaccttt cgccagcatc atcgccatca tgccgaccgg ccccagaccg accaccagca
    70141 cgttatcgct gccggaaact tcgccgcgca aaatcccttc ataggccgtg ccaacgccgc
    70201 aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc agatcttttt
    70261 cttccgccag cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa gccgcttttc
    70321 ctttgccagt acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa ccacagccag
    70381 aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct tgccccatcg
    70441 ccacaatctg cccgcacggt tcatgaccgt tgataaatcc ctggtataac ggtttatcgg
    70501 gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt ccgcaaatcc
    70561 cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc gcaacttccc
    70621 gcagatcgac ggttgaattt cctggtaaat aagctgccag catcgttttc ataaatccct
    70681 cattaacaat acgattaatt ttcatccctg ccgcacccgc gccaggggcg ttaaggttta
    70741 gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg ccaccacgat
    70801 gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca ccagaccgtt
    70861 accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac cgcgaccgcc
    70921 ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca gaccgtttgc
    70981 ggcacctgcg ttaccagaac cgaggcgcgc cgccaacaaa atgccggtca ccgccgctaa
    71041 taatcccgaa agggtaaaga taagaatgcg cacccgacga acgttgatgc cgcacaactg
    71101 cgccgccgta gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg ttttgcggct
    71161 gatgaacaca aacagcgcaa acaacacaat catgatcagc gcggatactg gcacaccgag
    71221 aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg gcagtgcgtt
    71281 ggtcataaac agccccattc cgcgcagggc gctccacagc cccagtgtgg caacgaaact
    71341 tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg cgcctaacag
    71401 caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa atgccaggca
    71461 caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg agataataat
    71521 cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca cgttcatctg
    71581 gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa gaatggcaat
    71641 caccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga gcgagacgct
    71701 cttgccctgc ggcaatggta atgacgaagc agacatcttc aggttcctcg gtaaaattca
    71761 tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg agtgaaactc
    71821 ctgcgagaac gtgccgtgct gtaataacag gatgcggtca cacaccagcg gcaactcctc
    71881 cacttcactg gagataaaca ccacgctttt tccttcggca gccagctcac ggacaatacg
    71941 gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat
    72001 ctggctggcg gcatagaccc aacgaccgat caccactttt tgctgattgc caccagaaag
    72061 cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc atccgctgca tcacctcttc
    72121 ggtcaggcgg cggatggtgg accattgcag cacaccgttg gtgctgattt tttgccgatt
    72181 ggtcagcact gtattttcgt caacgcccag ccagggaatg atccccgctt ctttgcggtt
    72241 ttctggcgta tagccaatgc cgcgtttcag catgtcgccg taatcggggc gcatgatttt
    72301 ctcgccgttg ataacaattt cgccctgttc atacgtctcc agcccaacaa tcgccttcag
    72361 caattcactg cgccctgccc ccagcaggcc agcaatgcca agcacttcgc cgcgacgtaa
    72421 cgtaaagctg atatcctcca gcttgggctt atggcgtaat gcacggactt ccagcacggc
    72481 ctgatcgaca atttcctgag gggctaccgg cgcaatatca acgtgatcgc gccctagcat
    72541 tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg ccacctgacc
    72601 atcgcgcata acggtggcac aggaggcaat gcggcgaatt tcttccatcc ggtggctgac
    72661 ataaatcacc gccacgccca gtgccgacat ctttttcacc gcgctgatca ccagttcaac
    72721 ttccgcactg gcaagcgaac tcgtaggttc atcaagaatg accacgcgcg gctcgccctt
    72781 catcacccgt gcaatttcca ccagttgctt ttgcgccggg cttagcgttg aaacaagttg
    72841 ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct gcgccatttg
    72901 caggtaatca atcatgccgt tgcggcgggg ccactgaccg aggcagaggt tttccgccac
    72961 cgtcagccct tccaccagac ttaactcctg ataaaccgca cgaaccccca gttcagcggc
    73021 acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc agatatcacc
    73081 gctatccggg cgttcactgc cggtaagcat tcgaatgaga gtcgatttgc ccgcgccgtt
    73141 tttacctaac agcgcacgaa cttcgccttt attgagcgtg aagttaacgt tatccagcgc
    73201 aacgacgccg gggtaacgct tatttcctgc caccactttt gctaccggga ctgcctctgt
    73261 tgccgtgaac atagggacct ctgcgaatca gcgattaggg cagaccatca acgtgcgttg
    73321 ccagccactg tttaccatct tccgttttgg tatagagatc gattggcacc tgaatcactt
    73381 tttcaccgtc ggcttgttta ttgataacct tcaatgtttg cgcgagaaca gcattgccca
    73441 ttttcttacc ggaaatatcc actaccgctt tcagcacctg attgttttcc agctcctgag
    73501 caatttcagt ggtcatatcc gaaccgaaaa cagcaatttt tccggcctga ttttgattac
    73561 gtaccgcttt taccgcgccg agtgtcgctc cgcccgattc acccataatg gcgttgagat
    73621 ccggcgtgga gataattagt ttttcaccaa cggaaatcgc tttatctaaa acagtccctt
    73681 cctgattagc gacaatttgc gcgccgggaa cgcgggattt taatacttct tcaaatcctt
    73741 tacgtcgctg cacacaaact tcaaaggctt cgcaattgat gacggcaatt ttcggctggt
    73801 caattttatt ggcaataaaa taatcggcgg cagcattacc cagtttttta ccaaattcca
    73861 gcggatcgcc gaccagatac gccgagacat atttatcgac acccttttga ttaatacagg
    73921 tgttgtagca aatcaccgga atacccgctt cactggcgcg acgaacggta cggctactgc
    73981 cattttcaga cactgccgat aaaataatgg catcgacatt acgcgccacg agggtatcaa
    74041 caaaggtact ttctttcgaa atatcaccct gggcgttagt ttcaattaac tgtacctgta
    74101 ctgaagaatc ttttgccgca tcctgaacgc cttgacgcac tccagcgtaa tatccctgag
    74161 tatcaaggta tattgcgcca atggtcattt ctttttccgc agccctggca aataaagcgc
    74221 tacctaatag cgtagccatt aataatagat tacgggttgt tctcattttt ttaggcatag
    74281 agcctcctgt agggttttta ttaacaacgg cttattctaa ttattttgtg atgagcggca
    74341 gcgcgtgccg ctcaaatatt acagttaata gggtttagtg aacgtagtta aaaataaaag
    74401 gcatcacagt ggtgaataat gccgctttcg gccaggccgg attaatcgcc gtcaattcag
    74461 cgcgagcaga ctcatattgc gcaacctccc cgagtcccgc ggacgccagc atcgccacca
    74521 tcaggcattt ttctttatgc tgctgttgta aatcgccata cagcgacaac agatcaggtt
    74581 gtgaaacagc aaagaaatcc gcctcaatac tggttttcgc catctcttgc gcccactgtt
    74641 tcatttcact aaacagttgc tgtgcggtgt gttgttcgcc cagcagtcgc agcgccattc
    74701 cttgccagaa gagataatca accggctgat cgttgtaata actgtggatg ttaatggtgc
    74761 gatcgccggt cgccgccaga cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt
    74821 tggcgcatat cgcctgccag aaccagatgt cgttatcagt ttgccccggt aaacggcctt
    74881 cgcttaaatt ctccggataa tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct
    74941 gtctggcatc aaggtgctgc caggcgcgta ataactggtt gaggataaac tgactggtga
    75001 ccttcccttc cccgccttcc cacgggtgga atttgcgcgt ggcgagaatg tccgccgctt
    75061 tgtctgcctg acccgtgaga tgccacaaat tgagcaactc tgctgtcatg tcgtcgcgtt
    75121 tcagcgcaat ttccagatta ttttccagac gcgccagtcg tttctccggt gtggctccgc
    75181 ttaacttatc aagcaaatcc cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat
    75241 aagcattatc aagataacgc gcggccagct cataatcgtg ttgcttattc cacgcatgga
    75301 tcgctaaccc gcgccagccg tcggcaaact ccggcgacat ctctacgcaa cgttgccata
    75361 aagtaatggc tttgttgtag ctgcgcttgt tgtagtagaa gcaggccagt aaatgacggg
    75421 caaaccagca ctcttcaata ctctccagcg ccgccacttc ttccagcgta ttcgggaaac
    75481 ggacaaactg cgggaagaca tcaatggctt ttgcgaccag ttcgccgcgt tcggcgttcg
    75541 gcagcaagct ggcttgtaga taaagcgata acgtgcgctg acagtccagt gcgttcagca
    75601 tctctgtcgc cagagtgggc attccccagt taatcagttg cccggcggtc atcagggcgt
    75661 taacgtcgcg tccctgacac agaccgcgcc actgcgcgag agctgattcg ctacgaccat
    75721 cgaaccagtt cagccaccac agagtggcgt tcagcggata atcgcgcagc agtttctcgc
    75781 gctgcacacg cgcgttgtcc tgacgaccac ttaacaccag cagcagatta tgcaggcaaa
    75841 gcacttcctg attggttggg caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt
    75901 cgaagttacc attacgcgcc gccagtcgtg ccaggccata atagccaccg gctttgctgt
    75961 tgccgctcca gaccgcacgc cagaaatcct cttcggcttg ttgatattgt ccctgacgtt
    76021 cgtaagcact ggcgcgaatc aaactcgcct gtccgcactg cggatttttg ttcagcgcat
    76081 gtgcgcgttt cagagcctga gtggcatacg ccaccgcttg cgggaaatcg gcacggttat
    76141 attccagcat cgccagcgcc aggttacagc ggtaatccag cggatccagc gccacgccgc
    76201 gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga tgctgaccga
    76261 taaaccaggc ttcatctgta ctggtaatgt cttgtgctga cagtggcgct ttggcgacgt
    76321 ccggcaacgg caactcttgc ggctgatgtt cctgataact cagtacaata ttgccgtcgg
    76381 catcggagag ctcaatggtc agcctttcag gattgatacc gtgcaacacg ccctggatgg
    76441 cggtcgcagg catcagtgcc acggcatcat caagtaacgc gttgcatttg ccgatttcgc
    76501 ggatcgccag gcggtatccg ttcaacggag agatggcata cagcccccac tcaatccccc
    76561 gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc atgcccaaag
    76621 aatgataagg caggaaatac tgctcaaaac gcttctcttc gtaagcatca agccaggtaa
    76681 aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg ccgttattgt
    76741 cggtcagact cttgtcccac gcctggccga attcactgtg tccccagctc cactgttttt
    76801 tacctggcgc gatatggtgg ttggcaacgt gtagcaaacc gccatcttca tcgtgacacc
    76861 acgcgccaac aaaatcgtac tgtgattttt cagccatata tgaggttgga acgggcacat
    76921 ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa gtgccggtgg
    76981 cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgccgtt acatccggcg
    77041 ggaagacgct ctgatgccct tccccccctt tcactgccgg gttggcccac cacaagaaat
    77101 gacgcggcgt ggcgttgccg ttatagacgc ggctggcaat ttccaacgcc gcccgttcag
    77161 gacgcagtgt gaaacctgtc atcacctgta aaccgtgcat cggctccgtt tcgccgaccc
    77221 acactgtctg tgcgccgtct tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg
    77281 tcgggcgatg gtgttgcggc cagttaaact caatcccgcc agagatccac ggtcccagca
    77341 gccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgt tgtttcactt
    77401 tatcccatgc gcgatgcaca cgaccgccca gttccggcag gatcatcact ttgatgtagt
    77461 cgttttccag ccacaccgcc tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg
    77521 tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt tccaggaaca
    77581 tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca
    77641 cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta aaagatttgt
    77701 ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat taactaaaca
    77761 aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc ataacaaatg
    77821 cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc tggcccggct
    77881 ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg
    77941 ggtggtgaat atcgacgatg aaagccagac gcgcgggcat agtagcggta catggctgat
    78001 tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca
    78061 ggttgctaat gcttgtttaa gtccgaaagg cgagtttgag tatttacaga ttgatgcacc
    78121 gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg
    78181 gccggaccgc accatcaatc tggcgctggc aatccacggt caggttgatc cggtgaccgg
    78241 cgtgtcgcaa accatgccgc aagcgccgtg gacaacgccg gtggaggtga aatatctgct
    78301 ggaagagaag ctcggcattc gggtgatggt cgataatgac tgcgtgatgc tggcgctggc
    78361 ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg
    78421 cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt atggtagcgg
    78481 acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg
    78541 ctgcctggaa accgtcgcct cgttaagtgc tttaaaaaaa caggcgcggg tatggctaaa
    78601 atcacaaccg gttaatactc agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc
    78661 tgcctggcag agtggagaac cgtggatcac cagctgggtt gaccgcagtg ccaatgccat
    78721 tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt ggttgtacgg
    78781 tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga caggatttaa
    78841 cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct tcgggcaatt
    78901 aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc agttacgaca
    78961 gatttgatgg cgcgataacg tagaaaggct tcccgaagga agcctttttt caacagatta
    79021 accgtttcgc ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga
    79081 tgcgctcgat aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca
    79141 gttctttcgc ttctgcttct ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa
    79201 taccggaggt cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga
    79261 tgttagcacg gcccagagcg gcgtctgctt ctttaccggt caggttttta tcaaccagat
    79321 caaccaggaa caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgttccagga
    79381 acacttctac catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag
    79441 gctccatcgc ttctttcaga gcaaccgctt taccagcgat tacatgcatc aacggaccgc
    79501 cctgaccacc agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt
    79561 tcgccaggat cagaccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa
    79621 cgtgagcatg aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg
    79681 ccatatcaac gaacaggtaa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt
    79741 ccaccacgcc ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg
    79801 cttgtttttc cagatcggcg tagtcgatat gaccggtagc atcgataccg taaggaacga
    79861 tgttgtacag tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg
    79921 ccaggttcat acccagaacg gtatcacctg gttccagcag cgcggtgtag accgcaaagt
    79981 tagcctggga gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg
    80041 cacgatcgat cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt
    80101 tgcccggata accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc
    80161 gcgggctggt gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt
    80221 cctgctccat agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta
    80281 acatccgcat ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt
    80341 tggacaacgg cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat
    80401 ttacgcaaac gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg
    80461 gaaacggatt tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga
    80521 aaaacaccaa agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac
    80581 atcaattaag atgcaaaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    80641 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    80701 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    80761 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    80821 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga
    80881 tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt
    80941 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    81001 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    81061 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    81121 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    81181 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    81241 gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg cgaagagggt gggcaggtat
    81301 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    81361 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct gccggtgttg
    81421 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    81481 tgaactggtt ccatgcggca gaaaatggcg atgttcacgc ctttgccgat gaagttaagg
    81541 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    81601 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaacaggaag
    81661 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    81721 ttgccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    81781 ttggcccgca taaggtgctg tgatttgatg ttgccggatg gaaacatccg gcaacccttg
    81841 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgtgcca
    81901 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    81961 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    82021 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    82081 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    82141 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    82201 aggtcgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    82261 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    82321 ccattctcgc cgcgtgggtg acgttgcctt tggtaatttg cagcagctta cgcaaatagt
    82381 tgagttcaaa ctgattacgt gcctcaacaa aggttggcag cgccgtgttt tcaccctcca
    82441 gcgcctgctc tactaatgca tcgctaataa ccggagatga ggtcagcgcc acgcactgtt
    82501 caatcacgtt gaccaactgg cgcacattac ccggccagct agcggtcatc aggcgtttca
    82561 tcgcatcagt ggagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    82621 ggtgatttgc cagaagcgga atgtcttctg tgcgctctgc cagagccgga attttcaggc
    82681 tgacaacgtt gaggcggtaa tagagatctt cacggaattc cccgcgcgcc atcgcttttg
    82741 gcaggtcgcg gtgagtggcg gaaataatcc gcacgtcgat atcaatatcg cggttgctgc
    82801 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgc aatggtgcgg
    82861 gcatatcgcc aatctcatcg agaaatagcg taccgccttc cgccgcctgg aataaacctt
    82921 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    82981 gcaactgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttacgcgggc
    83041 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctgg ccattaatca
    83101 acacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    83161 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    83221 gcgcatcgtc aattgcctga tatagcgcgt ctttgtcgac aggcttggtg aggaaactaa
    83281 aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca gtaagaataa
    83341 ttaccggcat tcccggctgc actttctgga tttcagcaaa cagctgcata ccgtccattt
    83401 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    83461 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    83521 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    83581 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac cctcgtgcgg cgtatccggc
    83641 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcagt caggttttcc
    83701 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    83761 tccagctcgc tgtcgctggc ctgctggagc ttgccgtagc gttggcgctc ttccgccagt
    83821 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gagggcgaac ctgtgccggg
    83881 atctcggtac ttagcgcatc aatgcgcgcg accagctggc ggcgctcata cggcgtaatt
    83941 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    84001 tggcgagctt gctgacgtga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    84061 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    84121 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    84181 atggctggct tattgtgact ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    84241 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaat
    84301 gcctatccca gtgggcgtaa ttattgcagg cagtttggac atggacagcg cggagaaacc
    84361 ggagcgtaca cgtcgtacgt gaggatttcg agcactgccc ggggccgaac tggcaaataa
    84421 aatagcctga tgggataggc tgttagcatt atttcgtgtt tttcgacgac ggcaattcaa
    84481 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    84541 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    84601 ggtggcttcc ttgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggtg
    84661 tgcctgtatt gatgacatca atataaaccc gtgcaccatg taagctgctg cgaaggcaaa
    84721 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    84781 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    84841 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    84901 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    84961 cgatcagttt ttgcaaattg cggctgctgc tatcaagaat gctcaccacc tctttttgct
    85021 ctggcgtaag cggcccgaca acctggtcag ccagtaattc agtgccctcg cgcatactcg
    85081 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgc tgggattcca
    85141 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    85201 gtccactgaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    85261 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    85321 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    85381 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    85441 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    85501 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    85561 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    85621 ccttcgcaag cgtaggatcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    85681 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    85741 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    85801 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    85861 caggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    85921 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    85981 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc
    86041 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    86101 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    86161 tggtaacgta ctgattatac gtcgctttga ggggtttatg gcttctccgt tatttgaatg
    86221 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agataatatt
    86281 aaaatcaatg agttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    86341 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    86401 ggatgcggcg taaatgccgc atccgggctg caaaaccaat gaactgatga cttaccccaa
    86461 ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca tcctcgcccc agttttccgg
    86521 atgccaggag ttgctgacag tacggaaaac acgttccggg tgcggcatca taatggtgac
    86581 tcgaccactt tcagtcgtaa tcgacgtaat accgttcggt gaaccgttcg ggttagccgg
    86641 gtaggtttca gtgactttgc cgaagttatc gacatagcgc agtgccacca gccctttgct
    86701 ttccagtgcc gccagatgcg ccgcatcacg cacttccacg cgcccttcac catgagagac
    86761 agcaatcggc atttgcgagc ccaccatccc ctgcaacagc agcgacgggc tttgggtcac
    86821 ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggta ttgcgcacaa aacgtggcca
    86881 caactcacta cctgggatca gttcgcgcag gttagacatc atctggcaac cgttacatac
    86941 ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca aactcatcgc gtacacggtc
    87001 attgaacagg attgacttcg cccaaccttc accggcaccc agcacatcac cgtaggagaa
    87061 accaccgcac gcgaccaggg cgtggaaatc ttccaggccc gtgcgtccag tcagcaggtc
    87121 actcatatgc acatcgattg catcgaagcc tgcacggtgg aaagctgccg ccatttcaac
    87181 atgcgagttc acgccctgct cacgcagcac agcaactttc ggacgtgcgc cagtggcaat
    87241 atacggtgct gccacatctt cgttgatatc gaacgacagt ttcacattca ggcccggatc
    87301 ggcgtcgttt gatttcgcct ggtgctcctg gtcagcacac tccgggttgt cacgcaggcg
    87361 ctgcatctgc caggtagttt ctgcccacca gacacgcaac gtggtgcggc tttcgctgaa
    87421 tacagtctgc ccgttggcgg taatcacaaa acggtcaccg gaaaccgcct gccctacata
    87481 atggacacaa tcagcaagcc catgctgtgc cagtacggac tcgaccgctt cacggtcagc
    87541 ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac aacgccgcca ggcgatcgtc
    87601 gcccagagag gcaatatccg cgttaatgcc gcaatgacca gcaaaggcca tttccgccag
    87661 cgttaccagc aggccgccat cagagcggtc gtggtatgcg agcagcttac gctgtgcaac
    87721 cagcgcctga atcgcgtcat agaagccttt cagttgcgcg acatcgcgta catctgccgg
    87781 tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt gcgcccagcg cgttattgcc
    87841 tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta gaaagctgcg gcgtgatggt
    87901 gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc agcgacagcg gcgacgtcat
    87961 ttcgcgctct tcgttacctt cctgccagcg ggttttcatc gacatagagt ctttacccac
    88021 cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc acggctttaa cggcttcata
    88081 caggcccgca tcttcaccag ggtggcctgc cgccgccatc cagttggcgg aaagtttaat
    88141 gcgtttgata tcgccaatct gtgttgcggc aatattggtt aacgcttcac cgaccgccag
    88201 acgggcagag gcggcgaaat ccagcagcgc aaccggcgca cgctcgccaa tcgccatcgc
    88261 ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg cagttagcga ccggcacctg
    88321 ccacggaccc accatctgat cgcgcgctac catgccagtt acgctgcggt caccgatggt
    88381 caccaggaaa gttttctccg ccacggtcgg cagatgcagt acgcgtttca ccgcgtctgc
    88441 aatggtgatc ccttcacgga ccagcgcatc gcccttcgct ttcagcgttt gtacatcgcg
    88501 ggtcatcttc ggcgttttac caagcaggac gtccagcggc agatcgatcg gctgattatc
    88561 aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc gcttcaccaa tcaccgcgta
    88621 gggtgcgcgc tcacgcttac acagttcgtc aaacagcggt aattgatcgg ctgcaaccgc
    88681 cagcacgtag cgttcctggg attcgttaca ccagatttcc agcgggctca tgcccggttc
    88741 gtcgcttaga atatcgcgca gttcaaattt accgccgcgt ccgccgtcgc tcaccagctc
    88801 cggcatcgcg ttagaaagac cgcccgcacc gacgtcgtgg ataaacagaa ttgggttagc
    88861 atcaccaagc tgccagcaac ggtcgatcac ttcctggcaa cgacgctcca tctccgggtt
    88921 gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac tgacctgacg ccatggaaga
    88981 cgccgctccg ccgccaagac cgatgttcat tgccgggccg ccgagaacga ccagcttcgc
    89041 accgacgttg atctcgcctt tttgtacgtg atcggcgcga atgttgccga tcccgcccgc
    89101 cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg ttgtggctgt tcactttttc
    89161 ttcataagta cggaagtagc cattcagtgc cggacgacca aattcgttgt taaacgccgc
    89221 gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg acaatacgct caggcttacc
    89281 gaaatcttct tcccacggct gttcgaagcc aggaattcgc aggttggaaa cggagaaacc
    89341 aaccaaaccc gctttcggct tcgcgccgcg cccggtggca ccttcatcgc ggatttcacc
    89401 gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc gtcgggtggt tgtgagtttc
    89461 gactttcatc agaatatgcg ccggttcctg atggaaatcg tagcggcccg tttcgtggtc
    89521 agcaaagtag cggcccactt cagaaccttc cattacggcg gcgttatctt tataagcaga
    89581 gagaacgtaa tctggcgtgg tttcgaaggt gtttttgatc atcttaaaca gcgatttcgg
    89641 ctgttgctca ccgtcgataa cccaatcggc gttaaaaatc ttgtggcggc agtgctcgga
    89701 gttcgcctgg gcgaacatat acagctcgat gtcgttcggg ttacgaccaa gctttgtgaa
    89761 agcatcctgc agatagtcaa tttcatcttc cgccagtgcc aggccaagac gcaggttagc
    89821 gtcgattagc gcctgacggc cctgccccag caaatcaacg ctggtgaccg gagtcggttg
    89881 atggtgtgca aacagctgct ctgcatcatc taaagcaaaa aagaccgttt ccatcatgcg
    89941 gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg gtcagcgtac cggcttctat
    90001 atagtaagca acgccgcgct caaggcggtt tacctgttgt agcccgcagt tatgggcaat
    90061 atcggtcgct ttcgaagacc agggagagat ggtgccagga cgcggggtca ccagcaggag
    90121 tttgccttgc ggggcgtggc tggcgagtgc cgggccatat ttcagcaggc gttcaagttg
    90181 tgcgtgctca tcatcgttta acggcgcatt gaggtcagca aaatggacat actcggcgta
    90241 aatattgtga accgggagcc tggcagcctg aaaacgtgcc agcagtttgt tgattcggaa
    90301 tgccgacagt gcaggcgaac cacgcagaat ttccatcata agtctctcgt cttctaagct
    90361 ttcggtgtac ccaagggggg gaaacgggcg tcattataaa gaatctgatg cgctgacgaa
    90421 accgtttgcg tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggctg
    90481 taataagttg ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt
    90541 tcactattat tacgtttttt caagctggga cgcgcacgac acagagaatt aactaattga
    90601 aaaaattaaa gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc
    90661 tctggccatc cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc
    90721 ggggagagtt gcgtgtgagc accattcata ctcccctgac ttataacgaa atcaacggga
    90781 aaccttttgg cctggattac gaactggcga aacagtttgc cgattacctc ggtgtaaaac
    90841 tgaaagtgac cgtgcggcag aatatcagcc agctgtttga cgacctcgat aatggtaacg
    90901 ccgacctgct ggcggcagga ctggtctata acagtgagcg ggtaaaaaat tatcagcctg
    90961 gccctaccta ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccgc
    91021 gcacactggg caacctgacg gcggagcaac tcaccgttgc accgggtcac gtggtggtta
    91081 acgatctcca aaccctgaaa gacacaaaat tcccggaatt aagctggaag gtagacgaca
    91141 aaaaaggctc tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg
    91201 ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg
    91261 atatcaccga tgaacaaccg gtgacttggt ttagcccgtt agatggcgat aatacccttt
    91321 ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg
    91381 aagagaaata cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac
    91441 gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa
    91501 ttgactggcg tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca
    91561 cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg
    91621 gcattacgga tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg
    91681 atatgatgag taaagtgccg gaaagtgtgc cggagaacga gcggatctgg tttgccctcg
    91741 ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca
    91801 aagggaatcc tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac
    91861 cctattacag caagctgact tacggctacg ctcgtggaca tgaagcctac gcttatgtcg
    91921 aaaatattcg taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg
    91981 ctacagaagc ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg
    92041 gcaaagagaa atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc
    92101 attctccctc tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg
    92161 aggattgcgc ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga
    92221 gcaacgccgc gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac
    92281 ccggatgatg cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac
    92341 caaagaccac gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt
    92401 caagcgtgac atacaacgtg gcgtcgatca gacgataatt ttgcatcacc agaccaccct
    92461 gccgcagggc catgatttct gcatgtgcgg tgggatcatg gcgaccaatc gggcggttcc
    92521 agccttcgcc gattacccga ttgttatgca ctaataccgc gccgaccggc acttcccgct
    92581 catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat ccagtattcg tggctaaatt
    92641 cgacttcaga caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct
    92701 atgctttact ctattccagt tgctggagtt caccgcgcgg ggttactcgc caacgatgct
    92761 gacagaaata aagcagcgga ttgtcctgat tactgtcgct atagccactg tacagccgca
    92821 gcggagtgcc gattttgcgc tccagttgtg cgaccttttc atgtcccaga caacgcatcg
    92881 tcaataccca accaccatag ccacgctgaa tttggctggc gataagatta acccgcggca
    92941 gccagggcgt atcgaaataa accgcttcaa ccagcggctg cggagagccg gtaatcaacc
    93001 agatatcagc atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg
    93061 cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt aaacgtgctt
    93121 cgctgtgacc aaaagtgcac ccccacagaa gcagactcat cggccagcgt gccgcacgac
    93181 cttttatcaa taacgcaatg gctataatcg gtaacaacgg caggacaagt aacgcattca
    93241 gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg
    93301 ttccatctaa gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg
    93361 gactgaaaca tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt
    93421 tccggcaaca atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg
    93481 caatggaata taaaattcac tcgcgtgtct ctcatattgc ctgttgtcgc cactatttca
    93541 tcgcgaggaa agatatgaac ggcttacttc gtatccgtca gcgttaccag gggcttgccc
    93601 agagtgataa aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa
    93661 gctctcagca actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc
    93721 aaaaactcgg ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa
    93781 gccagccgga atcgccctcc gtgcccattc ataaccaaat ccgcggtgat gatccgttac
    93841 ggctggtcgg cgaaaaactg attaaagaaa atactgccgc tatgtacgca acgctaaacg
    93901 ttaatagtga agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa
    93961 ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga
    94021 agattggctt caatgctgcc gcagtgcgcg atatgcatgc gctactcgca acagtacagg
    94081 cgtcgtcccc tgacgatctg ttattagcca tttcctacac cggtgtacga cgcgagttaa
    94141 acctggcggc agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta
    94201 ctccgaatgc cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag
    94261 cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg gatttgctgt
    94321 tcattgcgct gattcagcag gatctggaac tggcaccaga acgtattcgt catagtgaag
    94381 cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt
    94441 tgttttgaga gtttccttat ggcgttgtta atcactaaaa aatgcatcaa ttgtgatatg
    94501 tgtgaacccg aatgcccgaa tgaggcgatt tcaatgggag atcatatcta cgagattaac
    94561 agcgataagt gtaccgaatg cgtagggcac tacgagacac caacctgcca gaaggtgtgc
    94621 ccgatcccca atactattgt gaaagatccg gcgcatgtcg agacagaaga acagttgtgg
    94681 gataaatttg tgctgatgca ccacgcggat aaaatttaat attctactct ggaagtagag
    94741 tattaattat attactggga agccttaacg ccattatatt tatttaattg atgacattag
    94801 cataatcatt cactaagtta atttatatag tatctgccaa gacacttatt tatagttatt
    94861 aaaggcgcgt ccgattggtt caccggacgc accttaagta cgtttccttg tgttataaga
    94921 acagaaggat cagctgtaaa acagcaatga tgattttgat gacccgttta atcaggtatc
    94981 ggcaatcagt cattcgtttt tccttaaaca aggaatgcag ctgttcagat tagcccttac
    95041 atctccccaa aactgaacgt gcgagttatt gagggtgcat gctgcactcc acaccagagc
    95101 tttgacgaca ccactcgttt caatggggga attctgtggc atggtgtaaa gcacagcaaa
    95161 atcttcaata acgaagccaa ttttaatgta cttacgaatt ggcggtcacg ttaatctttc
    95221 catcaatatt gctttcttcg taaaggctcg agtttttatg ctaaagattg caagttgctt
    95281 gtaaaagata agtacactga tccataatcg ctgttgttga gggtgcatgc tgcacaaaat
    95341 taaagttaaa aagtaaaacc cccgttcctt accagttcgg gggttttact ttttaaagag
    95401 aacggtatta ttttaacttt caataattac cgtggcacaa gcatagtggc gttcatctgc
    95461 cagcgttaca tgcatattta caacgccgag cttttccgcc agttttaatg cctcacccca
    95521 tagccgtagc cgtggtttgc cgagctcatc attgaatact tcaaattgat taaacgccag
    95581 accattgcgg atcccggtgc caaacgcttt tgctgcggct tctttcacag caaaacgctt
    95641 cgccagaaaa cgcaccggct ggtggtgcgt tttccagatt gcccattcgt tatcgcttaa
    95701 tacgcggcgt gccaggcgct caccggatcg ggcgatcact gcttctatgc gagcgatctc
    95761 tacgatatcc gtgcctaaac ctaatattgc cattagccac gcgcttccag catcagacgc
    95821 ttcatttctg ccaccgcatc tttcagtccg gtcatcactg cacgaccaat aatggcatga
    95881 ccgatattca gttcatgcat ctcagggatg gcggcaatag ctttcacgtt gtgataggtc
    95941 agaccgtgac cagcgttaac tttcagaccg agactagcgg caaaggtcgc ggctttggcg
    96001 atacgcgcca gctcttgcgc ctgttcggcg tcagttttgg catcagcata gcaaccggtg
    96061 tggatctcga taaacggtgc gccaacctct gccgcagctt tgatctgctc ttcatcggcg
    96121 tcgataaaga gagaaacctg gatcccggca tctgccagac gtttgcaggc atcgcgcatt
    96181 ttgtcacgct gccctgcgac gtccaggccg ccttcggttg ttacttcctg acgcttttcc
    96241 ggtaccagac agcaaaaatg tggcttcgtc tcaacggcga tcgccagcat ctcttcggtc
    96301 accgccatct ccagattcat gcgggtatcc agcgtctgac gcaggatgcg cacatcacgg
    96361 tcagtaatgt ggcggcgatc ttcacgtaaa tgcacggtaa tgccgtccgc tcccgcctgc
    96421 tcagcaataa acgccgcctg caccggatcc gggtaagcag taccgcgtgc gttacgcagc
    96481 gttgcaatat ggtcaatgtt gacgcctaac agtaattcag ccatgacaat cctcatcatt
    96541 cataatgtgt tttcaccgtt cgcttaggca taaactgccg gaacagttcc ctgctcttta
    96601 atggtttacc gccaagatac ggcttaagcg ccatgcgggt aaagcgtttc gcggcgcgta
    96661 gtgtgtctgc gtcaggaaat tcccgcgcgt ttaacgcttt taactgcctt ccggtgaacg
    96721 ttttattgtc gataacgacg cttgcgataa accctttttc ttcgcgataa cgatacgtca
    96781 tggtgtcatc taccggctcg ccgctacccg cacaatgggt aaaattgacg ccatagccca
    96841 gatgcccgag cagtgccagt tcaaagcggc gcagcgcggg ttctggcgta ccagtggcac
    96901 ctgcaagaga ctgaatgcag tgcaagtaat cgaaaaagag ttcagagaag cgcgtctcgt
    96961 attccagtac gcgggagaga agttcgttga tgtacagacc gctgtaaagc gtgataccgc
    97021 ttaatggcag cgccagcgag acagcttcag cactgcgcag cgttttgact tcgccacgcc
    97081 cgccgaaacg tagcaagaga ggggtgaaag gctgtaatgc acctttcaga gtagagcgtt
    97141 tagagcgtgc gcctttggca accagacgca cgcgacccga ttcctccgtg aagacgtcca
    97201 gcatcaggct ggtttcgctc cacgggcgac tatgcaggac aaatgcgcgc tgccagcctt
    97261 ccatcggagt gactcttaaa gatcgtcaac gtaaccgaga ctgcgcagtg cgcgttcgtc
    97321 gtcggcccaa ccggatttca cttttaccca cagttcaagg tggacaggcg cttcgaacat
    97381 ttcctgcatg tctttacgcg cttcaatccc gatggttttg atcttggccc ctttgttgcc
    97441 aataaccatc ttcttctgcc cttcacgctc aacgagaatc aaaccattga tgtcataacc
    97501 accgcgttcg ttagagacga aacgttcgat ctccacggtc acggagtacg gcagttcggc
    97561 gccgaggaaa cgcatcagtt tttcgcggat gatttcagac gccataaaac gctgtgagcg
    97621 atcggtgatg taatcttccg ggaagtgatg ggtcgcttca gggagatgct tacgcacgat
    97681 tgctgcgata gtgtcgacat tcagcccggt ttcagcagag atcggcacga tatcgaggaa
    97741 gttcatctga cttgccagga actgtaagtg cggcagcaga tcggcttttt cctgcacgtt
    97801 atccactttg ttcaccgcga ggatcaccgg cgctttgcca tcgcgcagtt tgttgagcac
    97861 catttcgtcg tccggcgtcc agcgggtgcc ttcaacgaca aaaatcacca gctcaacgtc
    97921 gccaatagag ctgctcgccg ctttgttcat caggcggtta atagcgcgtt tttcttccat
    97981 atgcaggcca ggggtatcaa cgtagatcgc ctgatacgcg ccttcagtat ggatccccac
    98041 aatgcggtga cgagttgttt gcgctttgcg ggaggtgatg gagatcttct gccccagcag
    98101 tttgttcaac aatgtggatt tgccaacgtt cggacgtccg acgatggcaa taaatccgca
    98161 gtaactttta tcgatgctca ttccagctcc agttttttca acgcctgttc ggcggcagcc
    98221 tgctcagcct tacgacggct tgaacctgtg ccaaccaccg gttcactcag gccgctgacc
    98281 tggcagtgga tagtaaattc ctgatcgtgc gcttcgccac gtacctggac taccagataa
    98341 gtcggcagcg gcagatggcg accctgcaaa tattcttgca agcgcgtttt cggatctttt
    98401 tgtttatcgc ctgggctaat ttcgtccaaa cgagtttgat accagttgag gattaatttc
    98461 tcgacggttt gaatatcact gtcgaggaat acgccaccaa ttaatgcttc gacggtgtcg
    98521 gcgagaattg actcacgacg aaatccaccg cttttaagtt cacctggccc taaacgtaag
    98581 cactcgccta actcaaattc gcgcgccagt tccgccagcg tattgccacg gaccagcgtg
    98641 gcgcgcatcc ggctcatatc gccttcatcc acacgaggga aacggtgata aagcgcattg
    98701 gcgataacgt agctcagaat agagtcgcct aaaaattcta aacgctcgtt atgtttgctg
    98761 ctggcactac gatgagttaa tgcctgctgc aacagttcct gatgattaaa agtgtagccc
    98821 agcttccgtt gaagccgatt aattacgatg gggttcatgc gataccaata aataaatgcg
    98881 tcaacaattc agcacacgaa acagacctga tatacatggc tctgctaact gcttcgctgc
    98941 agtttcagtt taccggtata tggggaccaa cgctgtttcg tgtgccgtgg caacctggag
    99001 gtgccaacct taaacttcgg gggaatattc tatacacaac gacgggggat gtcgttagcc
    99061 acgggagatt tatctcataa ataattcacg ttgtcgccat aacggcgaca acgtgaacga
    99121 agatgggcta ttaatggatg ccgccaatgc gacttaagcg cacacctgtc ggccattcgc
    99181 cttcttgctt atcgaaactc atccagatag ccgttgcccg accgaccaga ttcgcttcag
    99241 gcacaaaccc ccaataacgg ctgtccgcgc tgttgtcgcg gttgtcgccc atcatgaagt
    99301 attgtcccgg aggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc
    99361 ccacctgatc ctgcgcaatc ggcactgtca gaattcggtg cgtcacatca cccagtgtct
    99421 ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga
    99481 atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt
    99541 ccacgtttga gtaggtgacc ggcagcgcgt tttcacacgc ctggccggaa ctgcatcccg
    99601 gttgaatcgt cagctctttc gagaccggat cataagtaac tttatcgccc ggtaaaccca
    99661 ccgcgcgctt gatgtaatca agctttggat cttccggata tttaaagacc acgatatctc
    99721 cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat
    99781 aagcaaactt ttctaccaga ataaaatcac cgattaacag agtcggcatc atcgaacctg
    99841 acgggatctg gaacggttca taaataaacg aacgcacaat caatacgata gccagtaccg
    99901 gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaaccttt ttcaacgttg
    99961 ctttatccag tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag
   100021 gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca
   100081 gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga
   100141 cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc
   100201 gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat
   100261 aacatttagc cagtacgttt ttacgcagct gtttcacggt ggatcgcgca ataatgtgcg
   100321 taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct
   100381 tctccaccaa ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc agcgccaacg
   100441 catcaacacg ttcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc
   100501 gtttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga
   100561 agtcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt
   100621 aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg ttgccgagat
   100681 atgcctgcgg cagcagcatg tgacattcgg caatcggctc gcgcagttcg tagatgttat
   100741 ttaccgctgg cagcttggat ggactgtcga cgtaaataac ttctcttgac gtggtttcaa
   100801 cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac
   100861 gttcctggat gatctccatg tgcagcaggc cgaggaagcc gcagcggaaa ccaaagccca
   100921 gcgcgctgga gctttccggc tcatagaaca gtgaagcatc gttcaggctg agtttaccca
   100981 gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct
   101041 gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta
   101101 aggtatcgcc gactggagcg ccgtggatat ctttaatcgc acataccagc cagcctactt
   101161 cgccacattt cagttcagtg cggtcaacct gtttcggcgt gaagatgccc agacggtcgg
   101221 cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt
   101281 ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccatgag tcgataatta
   101341 gtgcctgcaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt
   101401 cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg tcagtggcgt
   101461 cgatgccgac gatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt
   101521 caatcttgtt cagtactggc accacttcga gatccatttc catggcggtg tagcagtttg
   101581 ccagggtttg cgcttccacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg
   101641 cagccagcga acgggaaact tcataggaga agtctacgtg gcccggggtg tcgataaagt
   101701 taagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga
   101761 tggtaatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac
   101821 ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt
   101881 caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct
   101941 taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat
   102001 agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt
   102061 gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca
   102121 ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca atgccacgct
   102181 taagatgatc ggttgccatt ctgcccgggc agcaaactta cgcgagtagc cgcgcgcaat
   102241 caggaacccg ccaataccac cgaggatcgc accacataat gctgcgacgt cagaagcaaa
   102301 gagtagctga aatagcgaag cgataaggaa taatcccacc agcggcgaca tataaaccag
   102361 taatgcggag ctaagcaggc tgccttcggc gatccccaat tccacttttt gccccggcac
   102421 taacggttca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttattcaa
   102481 cacgcggcta ccgcaaccgg cgcgtgaagc gcagctgctg catgaagctt taacatcaca
   102541 actgaccagc gcctgcccgt tttgccagga gacgacggta gcccactctt tgatcattgc
   102601 gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg
   102661 acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc
   102721 aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa gctgaataat
   102781 ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcga
   102841 ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct
   102901 ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccag cgtctgcata
   102961 ctgctactga tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc cagcgtttca
   103021 ccatcgcgat caaggagatc aaccctcatc ggtaatttcg attcgatgtc catccacacg
   103081 atgtagctgt agcgtgtacc atctcgggca accacgcgaa tgacttcgca aagacgatca
   103141 gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg
   103201 gtatagatga gcgatggcag agaatcaaca atgtaatcgc cattaagcgt gaacggttca
   103261 agtcccggtt caaaatagct gatttcattg ccacgctgta ccacttcccg gcgcgggcca
   103321 tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga
   103381 gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg
   103441 gccaggttca tctgctgtaa taacgccccg gacgcgggag tggccgaggc gttagcagag
   103501 aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt
   103561 gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgggtt tgcgcctgct
   103621 caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat
   103681 taatgcgacg acgctgctcc tgtacctgct gctgttgacc attgtttgcg gtcgcttcag
   103741 aaggtactcc caggcttacc gggctggctt tacccatcat cggcagtgta ttaaataccg
   103801 gcgtttcggg ctgctgggac gtttcagatt gtccattata gtgctggacg ccaacgataa
   103861 ctgcaagcga tacgcatgct gctacgccca tttgggtaag ctgtgccgcc cacggacgta
   103921 ctttctgcca gaatggcatt ttctgccatt gatgcggcgc aggctgggct tccgggatca
   103981 atgtcgccgg ttgacgtact ggctcttctt caatggcggc catcacgcgt gaagagatat
   104041 cgaaatggag cacctcggga gtatcacccc gcattgagtc acggattaag tgatagcttt
   104101 cccaggtttt ctgcatttct gggttatgag ccagttcgtt aagcagctca ctatccagcg
   104161 tttcgccatc cattaaagcg gaaagttgtt ctttctgcat gcctaatacc cttatccagt
   104221 atcccgctat cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag cttccctcgc
   104281 tcggaagata cgtgaacgca ccgtacctac cggacaatcc atgatagcgg ctatctcttc
   104341 atagctcagg ccatccagct cccgcaaggt tattgccatg cgcaaatctt ccgggaggga
   104401 ctcaatagtt cggaaaacta tctgtctcag ttcttctgac aacattaagt tctcagggtt
   104461 cgaaatttct ttcaacgcgc cgccactttc gaagttttca gcttcaatgg catccacatc
   104521 actggaaggt ggacgacgcc cctgagcaac caggtaattt ttcgctgtat ttacagcaat
   104581 ccgatacagc catgtataaa aagcgctatc tccccggaac gaatccagcg cacgataggc
   104641 tttaataaaa gcttcttgta ccacatcggg aacatcaccc gacggcacat agcgggaaac
   104701 cagactcgcc actttatgct gatagcgcac taccagtagg ttaaaggctt tctgatctcc
   104761 cttctggacc cgttcaacca ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt
   104821 ctccccaaac caaatttcca cgcgctatcg aaacgccact ccattagctg caatttgagc
   104881 aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg tttttgtttg
   104941 tcatgctgta gacggatcat tatctatcat tataagtcta cagaatctga acatcgcatt
   105001 atctgtgtag aaatgcccat ttaactgcct gaagagtaac ccaacggcct ttttatttca
   105061 ccacctaatc ctccaccagc cagtaacttc tctttttctc gccgccctgc gtcagcgtgt
   105121 ttagccactg taacaaatat taaaatagca ggtgtttatt cgcacaacat gatgctatgc
   105181 tgaccaaacg gtgtttagta aattaaacaa agaaaatgaa tactctccca gaacattcat
   105241 gtgacgtgtt gattatcggt agcggcgcag ccggactttc actggcgcta cgcctggctg
   105301 accagcatca ggtcatcgtt ctaaggcccg gtaacggaag gttcaacatt ttatgcccag
   105361 ggcggtattg ccgccgtgtt tgatgaaact gacagcattg actcgcatgt ggaagacaca
   105421 ttgattgccg gggctggtat ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca
   105481 cgatcctgtg tgcaatggtt aattgaccaa ggggttttgt ttgataccca cgttcaaccg
   105541 aatggcgaag aaagctacca tctgacccgt gaaggtggac atagtcaccg tcgtattctt
   105601 catgccgccg acgccaccgg tagagaagta caatccacgc tggtgagcaa ggcgcagaac
   105661 catccgaata ttcgtgtgct ggagcgcagc aacgcagttg atctgattgt ttctgacaaa
   105721 attggcctcc cgggcacgcg acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa
   105781 acggtggaaa cctgccacgc aaaagcagtg gtactggcaa ccggcggtgc gtcgaaggtt
   105841 tatcagtaca ccaccaatcc ggatatttct tctggcgatg gcattgctat ggcgtggcgc
   105901 gcaggctgtc gggttgccaa tctcgaattt aatcagttcc accctaccgc actgtatcac
   105961 ccacaggcac gcaatttcct gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag
   106021 cgcccggatg gcacgcgttt tatgcccgat tttgatgagc gcggcgaact ggccccgcgc
   106081 gatattgtcg cccgcgccat tgaccatgaa atgaaacgcc tcggcgcaga ttgtatgttc
   106141 ctcgacatca gccataagcc cgccgatttt attcgccagc atttcccgat gatttatgaa
   106201 aaattgctcg ggctggggat tgatctcaca caagaaccgg taccgattgt gcctgctgca
   106261 cattatacct gcggtggtgt aatggttgat gatcatgggc gtacggacgt cgagggcttg
   106321 tatgccattg gcgaggtgag ttataccggc ttacacggcg ctaaccgcat ggcctcgaat
   106381 tcattgctgg agtgtctggt ctatggctgg tcggcggcag aagatatcac cagacgtatg
   106441 cctgatgccc acggcgtcag tacgttaccg ccgtgggatg aaagccgcgt tgagaaccct
   106501 gacgaacggg tagtaattca gcataactgg cacgagctac ggctgtttat gtgggattac
   106561 gttggcattg tgcgcacaac gaagcgcctg gaacgcgccc tgcggcggat aaccatgctc
   106621 caacaagaaa tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg
   106681 cgtaatctgg tacaggttgc cgagttgatt gttcgctgcg cgatgatgcg caaagaaagc
   106741 cgggggctgc attttacgct ggattacccg gaactgctca cgcattccgg tccatcgatc
   106801 ctttcccccg gcaatcatta cataaacaga taaaaagcct gggtcagcgc cgtatacgct
   106861 tcggaatagt tctggtctgg cccacgaatg actaagcgat cgctaaagca ttctcccgcc
   106921 tgcggggaga atgccagcag cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc
   106981 gtccgcaaac gtaaatgcca gcccatgctt aatgccagtt cagtaaaacc attaccaatc
   107041 tgctctggca gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc cgcacaggtc
   107101 agcagcgatg ggtgatcaag cgtagtggta tagcgagcct gttcccgttg aggtgtcgcg
   107161 cactctactc cctgctgata gtaaggtggg ttactgatga ttaaatcgaa gcgtactgtc
   107221 tgctgtgtga tccactgctg aatatccgcc gtatggacgt taatccgctc tgcccacggg
   107281 gactggttga tattttcctg cgcctgcgcg gcagcttcac tttccagctc aacggcatca
   107341 attatcacgc tgtcactggt tcgctgcgcc agcatcagtg ccagcaaccc gctacccgcg
   107401 ccgatatcaa ggcaacgttt caccccagcc accggtgccc atgcgcccag caaaatgcca
   107461 tccgttccca ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat
   107521 ccattacgac gaagcacgga tgtagactgt gacatgaaaa taaaaccttg caggaaaaac
   107581 ggcgatagca ccgggtgaga acaatacccg agaagcgata tccatacaaa cagatgaaga
   107641 ttgcagccgt aacgtctata atcagcgccc cacacagagg tagaacatga ctgtaacgac
   107701 tttttccgaa cttgaactcg acgaaagcct gctggaagcc ctccaggata aaggtttcac
   107761 tcgcccgacc gccattcagg ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact
   107821 cggttctgcg ccgacaggca ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca
   107881 cctgctcgat ttcccgcgta agaaatccgg tccgccgcgt attttgatcc tcaccccaac
   107941 tcgcgagctg gcgatgcagg tggccgatca tgcccgcgaa ctggcgaaac atacgcatct
   108001 ggatatcgcc accatcaccg gcggcgtagc ctatatgaac cacgcggaag tgttcagcga
   108061 aaatcaggac atcgtggtcg ccacgaccgg acgtctgctg caatacataa aagaagagaa
   108121 cttcgattgc cgcgcggttg aaacgctgat cctcgacgaa gcagaccgta tgctggatat
   108181 gggcttcgct caggatatcg aacatattgc tggcgaaacg cgctggcgta aacagaccct
   108241 gctcttttcg gcaacgctgg aaggcgatgc gattcaggac tttgccgagc gtctgctgga
   108301 agatccggtg gaggtttctg ccaatccgtc cacccgtgag cgcaaaaaaa ttcatcagtg
   108361 gtattaccgc gccgatgacc tggaacataa aaccgcattg ctggtgcatc tgttaaaaca
   108421 gccggaagcg acccgctcaa ttgtgtttgt gcgtaagcgt gaacgtgtgc atgagctggc
   108481 aaactggctg cgcgaagcgg gcatcaacaa ctgctatctc gaaggtgaga tggtacaagg
   108541 caagcgtaac gaagcgatca agcgtttgac cgaaggtcgc gtaaacgtac tggttgcaac
   108601 cgatgttgcc gcgcgcggta tcgacattcc tgacgtcagc cacgtcttta acttcgatat
   108661 gccgcgcagt ggcgatactt atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa
   108721 aggcaccgca atttcgctgg tggaagccca tgaccatttg ctgctgggca aggttggccg
   108781 ttacattgaa gagccgatta aagctcgcgt tattgatgag ttacgcccga aaacgcgtgc
   108841 gccaagcgag aagcagaccg gcaagccatc gaagaaagta ctggcaaaac gcgccgagaa
   108901 gaaaaaagct aaagagaaag agaaaccgcg tgtgaaaaaa cgccatcgcg acaccaaaaa
   108961 tattggtaag cgccgtaaac caagcggaac gggcgtgcca ccgcaaacga cagaagagta
   109021 atctcaatgc cgggtttaag cctggtatta aaagtgctgg caaacgcaaa actgcctgat
   109081 gcgctacgct taacagtatc tacgtggctc atgcaatata ttgaatttgc acgatcttgt
   109141 aggccggata aggcgttcac gccgcatccg gcatgaataa agcgcacttt gtcgacaagt
   109201 taaatgccgg gtttaatccc gacattattt ttatccagcc acaaaactct cttcaacaca
   109261 ctgaataaaa gtgtgcatcg ccgggctaac cgcttttcca gcatggtgtg cgcacatggc
   109321 cgtaatggtc tgcgactgtt cgccaaaagg caattcaatt aattctccgc tctcaagctc
   109381 tttttccacc gcaaagcggg gcaagtaact gacaccgata ttcgccgcga cacaacgttt
   109441 gatactttca atgcttaaca gctcaatggt gttttccacc gtgatccgcc gctgacgcag
   109501 cgtgctctca aatatctgcc ggaagacaca ttgcggttcg ttgataataa agctacaggc
   109561 gttatgtctt cccggctcag taaaatcgac atctgcaatt tgcggtgaag ccaccagcac
   109621 cagcgattgt tcacccaact ctcgtcggtt cagggcatca tcattcccta cgcgataaaa
   109681 gacgccgaca tcggcctcat cattcagtag cgcatcacgg atcacgtaac agttcagcga
   109741 ctgcaacgat aaacgcactt ttggtgcgcg ttgccgaaaa cgctgcaaca cctgcggcat
   109801 tcggtaagag agcaacgttt cgcccgaaac aacgcgtagt tccccgtccg gatctgactc
   109861 ctttttggca gcctcacgaa gcgtatccat cactcgggta agttcataaa tgtgcggcag
   109921 caactttttt ccttcgcggg taaggcacat ccgtcggcca attttctcaa ataactggac
   109981 tgaaaactcc tgctcaagct gctgaatatg aaaagtcacc gtcgattgtg tacagcacaa
   110041 tttttgcgaa gctcgcaaaa aggaaccctc ttccaccacg gttttaagcg taataaaacg
   110101 gcgcagatcc ataaccccaa acctatcgaa aatatcgaat ctagaatata aaaacattca
   110161 tttttttaaa tgttccgtgt cgggtactgt ctaccaaaac agaggagata acaagtgaca
   110221 ccgacccttt taagtgcttt ttggacttac accctgatta ccgctatgac gccaggaccg
   110281 aacaatattc tcgcccttag ctctgctacg tcgcatggat ttcgtcaaag tacccgcgtg
   110341 ctggcaggga tgagtctggg atttttgatt gtgatgttac tgtgtgcggg catttcattt
   110401 tcactggcag tgattgaccc ggcagcggta caccttttga gttgggcggg ggcggcatat
   110461 attgtctggc tggcgtggaa aatcgccacc agcccaacaa aggaagacgg acttcaggca
   110521 aaaccaatca gcttttgggc cagctttgct ttgcagtttg tgaacgtcaa aatcattttg
   110581 tacggtgtta cggcactgtc gacgtttgtt ctgccgcaaa cacaggcgct aagctgggta
   110641 gttggcgtca gcgttttgct ggcgatgatt gggacgtttg gcaatgtgtg ctgggcgctg
   110701 gcggggcatc tgtttcagcg attgtttcgc cagtatggtc gccagttaaa tatcgtgctt
   110761 gccctgttgc tggtctattg cgcggtacgc attttctatt aacgaaaaaa agcggaagag
   110821 gtcgccctct tccgcttagt aacttgctac ttaagcctta caggctttca gtaaaggtac
   110881 gagcgataac gtcgcgctgc tgttccggag tcagagagtt aaagcgaact gcataaccgg
   110941 atacacggat ggtcagctgc ggatattttt ccggatgctt aactgcatct tccagagttt
   111001 cacgacgcag aacgttaacg ttcaggtgtt gaccaccttc aacgcgaact tctggtttca
   111061 cttctactgg aacttcacgg tattcaatgt cacccagttt gcttactgca accacttcat
   111121 cttctgcata acctgctttt gcaacgatgc aacgtgcttc gcctttttcg ctgtccagca
   111181 gccagaaaga gttcagcaga tcgtcgttag cggctttagt aatctggata cctgtaatca
   111241 tgtgatgcct ccccggcaaa attatttgat ttgttcagcc tgtcgcggcc aattggtaaa
   111301 accattgttg cttgagtgta tatatactcc tcaaccaccc ttgattcttt gatttaaatc
   111361 aataaaaacc acgcatcgat tgtggtcgta aatggatttt attgttttac atcaacttat
   111421 gcgggtgtga aattttacca atttacattt ttttgcactc gtttaagtct aaaaaatgag
   111481 catgattttg ttctgtagaa agaagcagtt aagctaggcg gattgaagat tcgcaggaga
   111541 gcgagatggc taacgaatta acctggcatg acgtgctggc tgaagagaag caacaacctt
   111601 attttcttaa tacccttcag accgtcgcca gcgagcggca gtccggcgtc actatctatc
   111661 caccacaaaa agatgtcttt aacgcgttcc gctttaccga gttgggtgac gttaaagtgg
   111721 tgattctcgg ccaggatcct tatcacggac cgggacaggc gcatggtctg gcattttccg
   111781 ttcgtcccgg cattgccatt cctccgtcat tattgaatat gtataaagag ctggaaaata
   111841 ctattccggg cttcacccgc cctaatcatg gctatctcga aagctgggcg cgtcagggcg
   111901 ttctgctgct caatactgtg ttgacggttc gcgcaggtca ggcgcattcc cacgccagcc
   111961 tcggttggga gacgtttact gataaagtca tcagcctgat taaccagcac cgcgaaggcg
   112021 tggtgttttt gttgtgggga tcacatgcgc aaaagaaagg ggcgattata gataagcaac
   112081 gccatcatgt actgaaagca ccgcatccgt cgccgctttc ggcgcatcgt ggattctttg
   112141 gctgcaacca ttttgtgctg gcaaatcagt ggctggaaca acgtggcgag acgccgattg
   112201 actggatgcc agtattaccg gcagagagtg agtaaatttg cggggaaatg ccggatggca
   112261 gagttgccac ccggctgatt tatcaggctt tattctgacg ccaccattca ccaagcaaaa
   112321 cgccggttgc gacagagata ttcagcccgg caacgttgcc cgtaccgtca atcttcacgc
   112381 gcagatcgtt cggatcgcgt gcggcatccg gtaacccttc atattcctga cccagcacca
   112441 gtaccatttt cgctggcaga ctagttttga acagcggttt accctgctcg ctggaagtgg
   112501 tcactacggt gtaacctgcc tgacggaaat catccagcac gttaacaatg ttgtcgccag
   112561 taatcggctg aacgtgctct gcgccgcctt ctgcggtacg gatagccgcc cccgactcca
   112621 gcagtgccgc atcctgcacc acgacacctt tcacgccgaa gtgcgcgcag ctgcgcatca
   112681 tgccacccag gttatgcggg ttagattcgt tttccagtgc cagaacgcaa tcctgcgcgc
   112741 ctgcctggct tacccactgc tgcacggttg taccgttacg ctttttgatc aagaagcaaa
   112801 cgccgccgtg atgttccgtg cctgacgctt ttgtcaattc cgcttcatcc accacatggt
   112861 acgctttgcg gtttgctgcc atccagcgca aggcttcttt aaaacgcggc gttacactct
   112921 ggataaacca ggcgcgaaca atcgcttccg gacggctctg gaaaagtgcc tgacatgcat
   112981 tttcgccgta gacgcgggtt tcttccgcac gctgacgacg caacacttcc ggatcaataa
   113041 aacttttacc actgatgcca ccgtgatcgg ccttttccgg cgtctcatca cccggcgcgc
   113101 gggaaaccgt gcgccacggc gaatcttccc acttgcggtc acggggctga ctctgtttgt
   113161 catcgcgggc ggggcgacgg ccaccgtcag cacgagattt tcctggacgc ccgccccctt
   113221 tcccggtacg cgggttgtgg gtacgtttat cagaatcatc atcactgcgg acatacatca
   113281 ctttgacctt gccgctttta cctttcattt catcgttcat gcttttctcc accagcgctg
   113341 cgcgaagcgc gcagattacc cgaagtccgc gcggttcgcc atgatttcgt accaaagcct
   113401 gcgactatca tacctattga ataaaacaga ttgttgtctg gaacaatgtc cccgataata
   113461 tgtaacatat tagaaacata ccggcgtcgt tgccgataag tctccttact catcccgagg
   113521 ttagttatga ataccgtttg tacccattgt caggccatca atcgcattcc cgacgatcgg
   113581 atcgaagatg cggcaaaatg cggacgctgc ggtcacgact tgtttgacgg agaggtgatt
   113641 aatgcgaccg gtgaaacact cgacaaattg ctgaaggatg atctacctgt ggtgatcgac
   113701 ttctgggcac cgtggtgcgg cccctgccgt aatttcgcac caatttttga agatgtcgcg
   113761 caagagcgta gcggtaaagt gcgctttgtg aaagtgaata ccgaagctga acgtgaattg
   113821 agcagtcgct ttggaattcg tagtataccg acgatcatga ttttcaaaaa cggtcaggtt
   113881 gtcgacatgc ttaatggcgc agtaccgaaa gcgccgttcg atagctggct gaacgaatct
   113941 ctttaatctt accggggcac atcttgtgcc ccgttttctc ctctgcgaca atggcgtttt
   114001 ttcgacgctc tcttatgacc gaaaacgctg ttctccagtt acgcgccgag cgtattgcgc
   114061 gcgcaacacg tccttttctt gcccgcggta atcgcgttcg tcgctgccaa cgctgtcttt
   114121 tgccagagaa attatgtctc tgttcgacaa ttaccccagc acaagcaaaa agtcgcttct
   114181 gtttgctgat gttcgacacc gagccaatga agccaagtaa caccgggcgt ctgattgctg
   114241 atattttgcc tgataccgtt gcgtttcaat ggtcgcgtac cgacccctcg caagacttgc
   114301 tggatctggt acaaaacccg gactatcaac caatggtggt ctttcccgcc tcgtatgctg
   114361 atgagcaacg ggaagtgatc ttcacaccac ctgccggtaa gccaccgctg tttatcatgc
   114421 tcgatggtac ctggccggaa gcgcgcaaga tgtttcgtaa aagtccgtat ctggataatc
   114481 ttcccgtcat ttccgtcgat ctttcccggc tttctgccta tcgcctgcgt gaagcccagg
   114541 ctgaaggcca atattgtact gccgaggtag ccatcgcact gttagatatg gccggcgata
   114601 ccggggcggc agcagggtta ggcgagcttt ttacccgctt taaaacacgc tatctggcag
   114661 gaaaaacgca acatctgggt agcatcacag cagaacagtt agaaagcgtt taaaatcatt
   114721 cggtcacttc tgcgggagac cggtatgagt cagcgaggac tggaagcact actgcgacca
   114781 aaatcgatag cggtaattgg cgcatcgatg aaacccaatc gcgcaggtta cctgatgatg
   114841 cgtaacctgc tggcgggagg ctttaacgga ccggtacttc cggtgacgcc agcctggaaa
   114901 gcggtgttgg gtgtgttagc ctggccggat attgccagct tgccctttac acccgacctt
   114961 gcggttttat gtaccaatgc cagccgtaat cttgctcttc tggaagagct cggcgagaaa
   115021 ggctgtaaaa cctgcattat tctttccgcc ccggcatcgc aacatgaaga tctccgcgcc
   115081 tgcgccctgc gccataacat gcgcctgcta ggaccaaaca gtctgggttt actggctccc
   115141 tggcaaggtc tgaatgccag cttttcgcct gtgccgatta aacgcggcaa gctggcgttt
   115201 atttcacaat cggctgccgt ctccaacacc atcctcgact gggcgcaaca gcgtgagatg
   115261 ggcttttcct actttattgc gctcggcgac agcctggata tcgacgttga tgaattgctc
   115321 gactatcttg cgcgcgacag taaaaccagc gccatcctgc tctatctcga acagttaagc
   115381 gacgcgcgac gctttgtttc ggcggcccgt agtgcctcgc gtaataaacc gattctggtg
   115441 attaaaagcg gacgtagccc ggcggcacag cgactgctca acacgacggc aggaatggat
   115501 ccggcgtggg atgcggctat tcagcgtgcc ggtttgttgc gggtacagga cacccacgag
   115561 ctgttttcgg cggtggaaac ccttagccat atgcgcccgc tgcgtggcga ccggttgatg
   115621 attatcagca acggtgctgc gcctgccgcg ctggcgctgg atgccttatg gtcacacaat
   115681 ggcaagctgg caacgctaag cgaagaaacc tgccagaaac tgcgcgatgc actgcctgga
   115741 catgtggcaa tctctaaccc gctcgatctg cgcgatgacg ccagcagcga gcattatgtc
   115801 aaaacgctgg acattctgct ccacagccag gattttgatg cgctgatggt tattcattca
   115861 cccagcgcag cggctcccgc aacagaaagc gcgcaagaat taattgaagc ggtaaagcat
   115921 catccccgca gcaaatatgt ctctttgctg acgaactggt gcggcgagca ctcctcgcaa
   115981 gaggcacgac gtttattcag cgaagccggg ctgccgacct atcgcacgcc ggaaggcacc
   116041 atcaccgcgt ttatgcatat ggtggagtac cggcgtaatc agaagcaact acgcgaaacg
   116101 ccggcgttgc ccagcaatct gacttccaat accgcagaag cgcatcttct gttgcaacag
   116161 gcgattgccg aaggggctac gtcgctcgat acccatgaag ttcagcctat cctgcaagcg
   116221 tatggcatga acacgctccc cacctggatt gccagcgata gcaccgaagc ggtgcatatt
   116281 gccgaacaga ttggttatcc ggtggcgctg aaattgcgtt cgccggatat tccacataaa
   116341 tcggaagttc agggcgtaat gctgtacctg cgtacagcca atgaagtcca gcaagcggca
   116401 aacgctattt tcgatcgcgt aaaaatggca tggccgcagg cgcggatcca cggcctgttg
   116461 gtgcaaagta tggctaaccg ggctggcgct caggagttgc gggttgtggt tgagcacgat
   116521 ccggttttcg ggccgttgat catgctgggt gagggaggtg tggagtggcg tcctgaagat
   116581 caagccgtcg tcgcactgcc gccgctgaac atgaacctgg cccgctatct ggttattcag
   116641 gggatcaaaa gtaaaaagat tcgtgcgcgc agtgcgctac gcccattgga tgttgcaggc
   116701 ttgagccagc ttctggtgca ggtttccaac ctgattgtcg attgcccgga aattcagcgt
   116761 ctggatattc atcctttgct ggcttctggc agtgaattta ccgcgctgga tgtcacgctg
   116821 gatatcgcgc cgtttgaagg cgataacgag agtcggctgg cagtgcgccc ttatccgcat
   116881 cagctggaag aatgggtaga attgaaaaac ggtgaacgct gcttgttccg cccgattttg
   116941 ccagaagatg agccacaact tcagcaattc atttcgcgag tcaccaaaga agatctttat
   117001 taccgctact ttagcgagat caacgaattt actcatgaag atttagccaa catgacgcag
   117061 atcgactacg atcgggaaat ggcgtttgta gcggtacgac gtattgatca aacggaagag
   117121 atcctcggcg tcacgcgtgc gatctccgac cctgataaca tcgatgccga atttgccgtg
   117181 ctggttcgct cggatctcaa agggttaggc ttaggtcgac gcttaatgga aaagttgatt
   117241 acctatacgc gagatcacgg actacaacgt ctgaatggta ttacgatgcc aaacaatcgt
   117301 ggcatggtgg cgctagcccg caagctcggg tttaacgttg atatccagct cgaagagggg
   117361 atcgttgggc ttacgctaaa tcttgcccaa cgcgaggaat catgagtaag gtactggaaa
   117421 tgttgaccac ttaatcggga ctggtgttat tattgcccgc ttatgtcgtc tgcattgcac
   117481 agaggaccct tcaatgaaca gagaagaaat gcactgtgat gttgtcaaaa tttaagcgta
   117541 ataaacatca acaacacctt gcccaactac ccaagatttc tcaatcagtt gatgatgtcg
   117601 atttctttta cgctcccgcc gacttccggg agacgctgct ggaaaaaata gccagcgcga
   117661 agcagcgcat ttgcattgtc gccctgtatc tcgaacagga tgacggtggc aaaggcattc
   117721 tgaacgcgtt gtatgaagct aaaaggcagc gtccggaact ggatgtgcgg gtgctggtcg
   117781 actggcatcg tgcacaacgt ggacgcattg gcgctgcggc atctaacact aacgctgact
   117841 ggtactgccg catggcgcag gaaaatccgg gcgtagatgt tccggtttat ggcattccaa
   117901 tcaatacccg tgaagccctt ggtgttctgc actttaaagg ctttatcatc gacgatagcg
   117961 tactttatag cggtgccagc ctgaacgatg tttacctgca tcagcacgat aaatatcgct
   118021 acgaccgtta tcatcttatc cgtaaccgta agatgtcaga cattatgttt gaatgggtta
   118081 cacagaatat tatgaatggc cgcggcgtta atcgtctgga tgatgttaat cggccaaaaa
   118141 gcccggaaat caagaacgat attcgtctgt tccgccagga gctgcgtgat gccgcttatc
   118201 atttccaggg cgatgccgac aacgatcagc tttccgtaac gccgctagtg gggctgggga
   118261 aatcgagtct gttgaacaag accattttcc atcttatgcc ttgtgccgag cagaaactaa
   118321 ccatctgtac gccatacttc aacctgccag caatccttgt gcgcaatatt atccagttgc
   118381 tgcgcgaagg gaaaaaggtc gaaattattg ttggtgataa aaccgcgaac gacttctaca
   118441 ttccggaaga tgaacctttc aagataattg gcgcattgcc ttatctctat gagatcaatc
   118501 tgcgtcgttt cctgagccgt ttgcagtatt acgtcaatac tgaccagcta gtggttcggt
   118561 tatggaaaga tgacgacaac acctatcacc taaaagggat gtgggttgat gataagtgga
   118621 tgttgatcac cggtaataac ctgaacccgc gcgcctggcg tctggatctg gaaaacgcca
   118681 ttttgatcca cgatccgcaa cttgagctgg cgccacagcg agagaaagaa ctggagctga
   118741 tccgcgagca taccaccatc gttaagcact atcgcgatct gcaaagtatt gccgattatc
   118801 cggtgaaggt tcgtaaactc atccgccgtt tgcgccgtat ccgcatcgac cgattaatta
   118861 gccgcatcct gtaatcacaa ccccgtcctg tacggggttt gttttttgga ggccacgttt
   118921 tgcgtattct ttttgtctgt tcgctattgt tactttctgg atgcagccat atggctaacg
   118981 atagctggag cgggcaggat aaagctcaac actttatcgc ctcggcgatg ctttccgccg
   119041 ccggaaatga atattcacag catcagggga tgagccggga tcgcagtgcc atgtttggat
   119101 tgatgttctc tgtcagtttg ggggcgtcaa aagagctttg ggatagccgc cccgaaggga
   119161 gcggctggag ctggaaggat ttggcctggg atgtcgccgg tgcaagcacc ggctataccg
   119221 tctggcaact gacccgtcac taaagacgca tccccttccc tttgcgatgt agcatcaaag
   119281 aaaccagaaa cgccaccacg gccatcaagg tcacatacca gaagaaggct gtttccattc
   119341 ctattgattt cagcgacaac gctacgtact ccgccgaacc accaaatata gcattagcga
   119401 ccgcatatga caggccaacg cctaatgcgc gaacctgtgc cgggaacatc tcagccttca
   119461 gtattccact gattgatgta taaaaactca ctatcagcag ggcacacatc accagaccaa
   119521 aagcggcata aggcgaggaa acgttttgca atgctgagag aataggaacg gtaaaaatgg
   119581 ctgccagcga accgaaacat aacattgagg tacggcgacc aatcttatcc gacagcgcgc
   119641 caatgagtgg ttgaataagc atgaatacaa acaatgcggc agtcataatg ccactcgcca
   119701 cgttggcatg cattcccgca gtatttacca gatacttctg catataagtg gtgaaggtat
   119761 agaaacaaag ggagcccgca gcggtaaaac cgagaaccat gatgaatgca cggcgattgc
   119821 gccataatcc tttcagagat ccagcttctt ttaaagcgcg cgtttcttgt tgcgaagttt
   119881 catctaactg acgacgtaac cacaacgcca caacagctaa cacagctcct aacgcgaaag
   119941 gaatacgcca tccccactct ctgagtgcag cgtcttccat ggtgtgttgt aaaaccacga
   120001 caaccagtag ggctagcagt tgtccgccga tcaacgtcac atactgaaat gatgcgtaaa
   120061 aacctttgcg cccttcaacg gcaacttcac tcatataggt ggcgctggtg ccatattctc
   120121 cgccaacaga taatccctga aataaacgcg cgagaagcaa taatgccgga gcccacgtac
   120181 ctatagtttc ataacctggg aggcaggcga taaccagcga tccgaaacac atcatacaca
   120241 ccgataacag catcgatttt ttgcgaccat gtttatcggc tatgcggcca aatagccaac
   120301 cgcctattgg gcgcatcagg aatcccgcag caaaaacacc tgctgtttgt agtagttgag
   120361 tcgtcgtgtt cccggaaggg aagaaaatgt gggcaaagta gagtgaacag aacgagtaga
   120421 catagaaatc gaaccactcg accagattac ctgaagaggc ccccacaatc gcccaaatgc
   120481 ggcgacgagt atcactactt gtcagtttgc tgtctgccgt tacagtactt tcagccatgc
   120541 cattatgtct cctgccgtaa tccgatgctt ttgtcggtcg cttttattta tttcttttgg
   120601 taaagaaaat gttatacatt tgttgcgtat cattatgcaa ccttaaccat gaatttagtt
   120661 agcaggaaaa tggttattga ggagcttaag gataaatttc tggtaaggag gacacgtatg
   120721 gaagtaggca agttggggaa accgcatccg ttgctgaatc tggcatatgt gggagtataa
   120781 gacgcgcagc gtcgcatcag gcattttttt ctgcgccaac gcaaaaaggc catcgtgact
   120841 gatggccttt
//
LOCUS       DAZFFI010000002       117973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFFI010000002 DAZFFI010000000
VERSION     DAZFFI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.002"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..597
                     /gene="yraH"
                     /locus_tag="ACHJ12_000127"
     CDS             13..597
                     /gene="yraH"
                     /locus_tag="ACHJ12_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4987980.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            677..1372
                     /gene="yraI"
                     /locus_tag="ACHJ12_000128"
     CDS             677..1372
                     /gene="yraI"
                     /locus_tag="ACHJ12_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4987981.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1402..3918
                     /gene="yraJ"
                     /locus_tag="ACHJ12_000129"
     CDS             1402..3918
                     /gene="yraJ"
                     /locus_tag="ACHJ12_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4987982.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3929..5020
                     /gene="yraK"
                     /locus_tag="ACHJ12_000130"
     CDS             3929..5020
                     /gene="yraK"
                     /locus_tag="ACHJ12_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4987983.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5063..5923)
                     /gene="rsmI"
                     /locus_tag="ACHJ12_000131"
     CDS             complement(5063..5923)
                     /gene="rsmI"
                     /locus_tag="ACHJ12_000131"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4987984.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5987..8023
                     /gene="lpoA"
                     /locus_tag="ACHJ12_000132"
     CDS             5987..8023
                     /gene="lpoA"
                     /locus_tag="ACHJ12_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4987985.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7981..8376
                     /gene="yraN"
                     /locus_tag="ACHJ12_000133"
     CDS             7981..8376
                     /gene="yraN"
                     /locus_tag="ACHJ12_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4987986.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8396..8986
                     /gene="diaA"
                     /locus_tag="ACHJ12_000134"
     CDS             8396..8986
                     /gene="diaA"
                     /locus_tag="ACHJ12_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4987987.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8996..9571
                     /gene="dolP"
                     /locus_tag="ACHJ12_000135"
     CDS             8996..9571
                     /gene="dolP"
                     /locus_tag="ACHJ12_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4987988.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9685..10725)
                     /gene="yraQ"
                     /locus_tag="ACHJ12_000136"
     CDS             complement(9685..10725)
                     /gene="yraQ"
                     /locus_tag="ACHJ12_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4987989.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRSTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10798..11433)
                     /gene="yraR"
                     /locus_tag="ACHJ12_000137"
     CDS             complement(10798..11433)
                     /gene="yraR"
                     /locus_tag="ACHJ12_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4987990.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11561..12079
                     /gene="yhbO"
                     /locus_tag="ACHJ12_000138"
     CDS             11561..12079
                     /gene="yhbO"
                     /locus_tag="ACHJ12_000138"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4987991.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12059..12502)
                     /gene="yhbP"
                     /locus_tag="ACHJ12_000139"
     CDS             complement(12059..12502)
                     /gene="yhbP"
                     /locus_tag="ACHJ12_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4987992.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12553..12855
                     /gene="yhbQ"
                     /locus_tag="ACHJ12_000140"
     CDS             12553..12855
                     /gene="yhbQ"
                     /locus_tag="ACHJ12_000140"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4987993.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(12842..13345)
                     /gene="yhbS"
                     /locus_tag="ACHJ12_000141"
     CDS             complement(12842..13345)
                     /gene="yhbS"
                     /locus_tag="ACHJ12_000141"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4987994.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13339..13863)
                     /gene="ubiT"
                     /locus_tag="ACHJ12_000142"
     CDS             complement(13339..13863)
                     /gene="ubiT"
                     /locus_tag="ACHJ12_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4987995.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14072..15067
                     /gene="ubiU"
                     /locus_tag="ACHJ12_000143"
     CDS             14072..15067
                     /gene="ubiU"
                     /locus_tag="ACHJ12_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4987996.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15076..15954
                     /gene="ubiV"
                     /locus_tag="ACHJ12_000144"
     CDS             15076..15954
                     /gene="ubiV"
                     /locus_tag="ACHJ12_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4987997.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            16160..17167
                     /gene="yhbW"
                     /locus_tag="ACHJ12_000145"
     CDS             16160..17167
                     /gene="yhbW"
                     /locus_tag="ACHJ12_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO4987998.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17285..18529)
                     /gene="mtr"
                     /locus_tag="ACHJ12_000146"
     CDS             complement(17285..18529)
                     /gene="mtr"
                     /locus_tag="ACHJ12_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4987999.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18683..20572)
                     /gene="deaD"
                     /locus_tag="ACHJ12_000147"
     CDS             complement(18683..20572)
                     /gene="deaD"
                     /locus_tag="ACHJ12_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4988000.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20565..20645)
                     /gene="yrbN"
                     /locus_tag="ACHJ12_000148"
     CDS             complement(20565..20645)
                     /gene="yrbN"
                     /locus_tag="ACHJ12_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4988001.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20752..21636)
                     /gene="nlpI"
                     /locus_tag="ACHJ12_000149"
     CDS             complement(20752..21636)
                     /gene="nlpI"
                     /locus_tag="ACHJ12_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4988002.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21745..23880)
                     /gene="pnp"
                     /locus_tag="ACHJ12_000150"
     CDS             complement(21745..23880)
                     /gene="pnp"
                     /locus_tag="ACHJ12_000150"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4988003.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24127..24396)
                     /gene="rpsO"
                     /locus_tag="ACHJ12_000151"
     CDS             complement(24127..24396)
                     /gene="rpsO"
                     /locus_tag="ACHJ12_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4988004.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24545..25489)
                     /gene="truB"
                     /locus_tag="ACHJ12_000152"
     CDS             complement(24545..25489)
                     /gene="truB"
                     /locus_tag="ACHJ12_000152"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4988005.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25489..25890)
                     /gene="rbfA"
                     /locus_tag="ACHJ12_000153"
     CDS             complement(25489..25890)
                     /gene="rbfA"
                     /locus_tag="ACHJ12_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4988006.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKEE"
     gene            complement(26054..28726)
                     /gene="infB"
                     /locus_tag="ACHJ12_000154"
     CDS             complement(26054..28726)
                     /gene="infB"
                     /locus_tag="ACHJ12_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4988007.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28751..30238)
                     /gene="nusA"
                     /locus_tag="ACHJ12_000155"
     CDS             complement(28751..30238)
                     /gene="nusA"
                     /locus_tag="ACHJ12_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4988008.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVHPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30266..30718)
                     /gene="rimP"
                     /locus_tag="ACHJ12_000156"
     CDS             complement(30266..30718)
                     /gene="rimP"
                     /locus_tag="ACHJ12_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4988009.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30925..31001)
                     /locus_tag="ACHJ12_000157"
     tRNA            complement(30925..31001)
                     /locus_tag="ACHJ12_000157"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30965..30967),aa:Met,seq:cat)
     gene            31349..32692
                     /gene="argG"
                     /locus_tag="ACHJ12_000158"
     CDS             31349..32692
                     /gene="argG"
                     /locus_tag="ACHJ12_000158"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4988010.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNCKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
     gene            32876..33829
                     /locus_tag="ACHJ12_000159"
     CDS             32876..33829
                     /locus_tag="ACHJ12_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4988011.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(33904..33990)
                     /locus_tag="ACHJ12_000160"
     tRNA            complement(33904..33990)
                     /locus_tag="ACHJ12_000160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33954..33956),aa:Leu,seq:gag)
     gene            complement(34005..34337)
                     /gene="secG"
                     /locus_tag="ACHJ12_000161"
     CDS             complement(34005..34337)
                     /gene="secG"
                     /locus_tag="ACHJ12_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4988012.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34565..35902)
                     /gene="glmM"
                     /locus_tag="ACHJ12_000162"
     CDS             complement(34565..35902)
                     /gene="glmM"
                     /locus_tag="ACHJ12_000162"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4988013.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPPEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(35895..36743)
                     /gene="folP"
                     /locus_tag="ACHJ12_000163"
     CDS             complement(35895..36743)
                     /gene="folP"
                     /locus_tag="ACHJ12_000163"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4988014.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(36833..38767)
                     /gene="ftsH"
                     /locus_tag="ACHJ12_000164"
     CDS             complement(36833..38767)
                     /gene="ftsH"
                     /locus_tag="ACHJ12_000164"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4988015.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(38867..39496)
                     /gene="rlmE"
                     /locus_tag="ACHJ12_000165"
     CDS             complement(38867..39496)
                     /gene="rlmE"
                     /locus_tag="ACHJ12_000165"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4988016.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            39622..39915
                     /gene="yhbY"
                     /locus_tag="ACHJ12_000166"
     CDS             39622..39915
                     /gene="yhbY"
                     /locus_tag="ACHJ12_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4988017.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40071..40547)
                     /gene="greA"
                     /locus_tag="ACHJ12_000167"
     CDS             complement(40071..40547)
                     /gene="greA"
                     /locus_tag="ACHJ12_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4988018.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            40795..42228
                     /gene="dacB"
                     /locus_tag="ACHJ12_000168"
     CDS             40795..42228
                     /gene="dacB"
                     /locus_tag="ACHJ12_000168"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4988019.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42268..43440)
                     /gene="cgtA"
                     /locus_tag="ACHJ12_000169"
     CDS             complement(42268..43440)
                     /gene="cgtA"
                     /locus_tag="ACHJ12_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4988020.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43456..44421)
                     /gene="yhbE"
                     /locus_tag="ACHJ12_000170"
     CDS             complement(43456..44421)
                     /gene="yhbE"
                     /locus_tag="ACHJ12_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4988021.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44548..44805)
                     /gene="rpmA"
                     /locus_tag="ACHJ12_000171"
     CDS             complement(44548..44805)
                     /gene="rpmA"
                     /locus_tag="ACHJ12_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4988022.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(44826..45137)
                     /gene="rplU"
                     /locus_tag="ACHJ12_000172"
     CDS             complement(44826..45137)
                     /gene="rplU"
                     /locus_tag="ACHJ12_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4988023.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45396..46367
                     /gene="ispB"
                     /locus_tag="ACHJ12_000173"
     CDS             45396..46367
                     /gene="ispB"
                     /locus_tag="ACHJ12_000173"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4988024.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46595..46873
                     /gene="sfsB"
                     /locus_tag="ACHJ12_000174"
     CDS             46595..46873
                     /gene="sfsB"
                     /locus_tag="ACHJ12_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4988025.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(46921..48180)
                     /gene="murA"
                     /locus_tag="ACHJ12_000175"
     CDS             complement(46921..48180)
                     /gene="murA"
                     /locus_tag="ACHJ12_000175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4988026.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48235..48489)
                     /gene="ibaG"
                     /locus_tag="ACHJ12_000176"
     CDS             complement(48235..48489)
                     /gene="ibaG"
                     /locus_tag="ACHJ12_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4988027.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(48649..48942)
                     /gene="mlaB"
                     /locus_tag="ACHJ12_000177"
     CDS             complement(48649..48942)
                     /gene="mlaB"
                     /locus_tag="ACHJ12_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4988028.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(48942..49577)
                     /gene="mlaC"
                     /locus_tag="ACHJ12_000178"
     CDS             complement(48942..49577)
                     /gene="mlaC"
                     /locus_tag="ACHJ12_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4988029.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(49596..50147)
                     /gene="mlaD"
                     /locus_tag="ACHJ12_000179"
     CDS             complement(49596..50147)
                     /gene="mlaD"
                     /locus_tag="ACHJ12_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4988030.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50152..50934)
                     /gene="mlaE"
                     /locus_tag="ACHJ12_000180"
     CDS             complement(50152..50934)
                     /gene="mlaE"
                     /locus_tag="ACHJ12_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4988031.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(50942..51751)
                     /gene="mlaF"
                     /locus_tag="ACHJ12_000181"
     CDS             complement(50942..51751)
                     /gene="mlaF"
                     /locus_tag="ACHJ12_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4988032.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51961..52938
                     /gene="yrbG"
                     /locus_tag="ACHJ12_000182"
     CDS             51961..52938
                     /gene="yrbG"
                     /locus_tag="ACHJ12_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4988033.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            52952..53938
                     /gene="kdsD"
                     /locus_tag="ACHJ12_000183"
     CDS             52952..53938
                     /gene="kdsD"
                     /locus_tag="ACHJ12_000183"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4988034.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            53959..54525
                     /gene="kdsC"
                     /locus_tag="ACHJ12_000184"
     CDS             53959..54525
                     /gene="kdsC"
                     /locus_tag="ACHJ12_000184"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4988035.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54522..55097
                     /gene="lptC"
                     /locus_tag="ACHJ12_000185"
     CDS             54522..55097
                     /gene="lptC"
                     /locus_tag="ACHJ12_000185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4988036.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55066..55623
                     /gene="lptA"
                     /locus_tag="ACHJ12_000186"
     CDS             55066..55623
                     /gene="lptA"
                     /locus_tag="ACHJ12_000186"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4988037.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            55630..56355
                     /gene="lptB"
                     /locus_tag="ACHJ12_000187"
     CDS             55630..56355
                     /gene="lptB"
                     /locus_tag="ACHJ12_000187"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4988038.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56403..57836
                     /gene="rpoN"
                     /locus_tag="ACHJ12_000188"
     CDS             56403..57836
                     /gene="rpoN"
                     /locus_tag="ACHJ12_000188"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4988039.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            57859..58146
                     /gene="hpf"
                     /locus_tag="ACHJ12_000189"
     CDS             57859..58146
                     /gene="hpf"
                     /locus_tag="ACHJ12_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4988040.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58264..58755
                     /gene="ptsN"
                     /locus_tag="ACHJ12_000190"
     CDS             58264..58755
                     /gene="ptsN"
                     /locus_tag="ACHJ12_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4988041.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            58801..59655
                     /gene="rapZ"
                     /locus_tag="ACHJ12_000191"
     CDS             58801..59655
                     /gene="rapZ"
                     /locus_tag="ACHJ12_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4988042.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            59652..59924
                     /gene="npr"
                     /locus_tag="ACHJ12_000192"
     CDS             59652..59924
                     /gene="npr"
                     /locus_tag="ACHJ12_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4988043.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60138..60770
                     /gene="yrbL"
                     /locus_tag="ACHJ12_000193"
     CDS             60138..60770
                     /gene="yrbL"
                     /locus_tag="ACHJ12_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4988044.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(60767..61495)
                     /gene="mtgA"
                     /locus_tag="ACHJ12_000194"
     CDS             complement(60767..61495)
                     /gene="mtgA"
                     /locus_tag="ACHJ12_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4988045.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61492..62145)
                     /gene="elbB"
                     /locus_tag="ACHJ12_000195"
     CDS             complement(61492..62145)
                     /gene="elbB"
                     /locus_tag="ACHJ12_000195"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4988046.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62375..64711)
                     /gene="arcB"
                     /locus_tag="ACHJ12_000196"
     CDS             complement(62375..64711)
                     /gene="arcB"
                     /locus_tag="ACHJ12_000196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4988047.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKTEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(64807..65736)
                     /gene="yhcC"
                     /locus_tag="ACHJ12_000197"
     CDS             complement(64807..65736)
                     /gene="yhcC"
                     /locus_tag="ACHJ12_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4988048.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            66411..70871
                     /gene="gltB"
                     /locus_tag="ACHJ12_000198"
     CDS             66411..70871
                     /gene="gltB"
                     /locus_tag="ACHJ12_000198"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4988049.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVSAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            70884..72302
                     /gene="gltD"
                     /locus_tag="ACHJ12_000199"
     CDS             70884..72302
                     /gene="gltD"
                     /locus_tag="ACHJ12_000199"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4988050.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72486..73613
                     /gene="yhcG"
                     /locus_tag="ACHJ12_000200"
     CDS             72486..73613
                     /gene="yhcG"
                     /locus_tag="ACHJ12_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO4988051.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEVLINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(73673..74137)
                     /gene="nanQ"
                     /locus_tag="ACHJ12_000201"
     CDS             complement(73673..74137)
                     /gene="nanQ"
                     /locus_tag="ACHJ12_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4988052.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74134..75009)
                     /gene="nanK"
                     /locus_tag="ACHJ12_000202"
     CDS             complement(74134..75009)
                     /gene="nanK"
                     /locus_tag="ACHJ12_000202"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4988053.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75006..75695)
                     /gene="nanE"
                     /locus_tag="ACHJ12_000203"
     CDS             complement(75006..75695)
                     /gene="nanE"
                     /locus_tag="ACHJ12_000203"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4988054.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(75743..77233)
                     /gene="nanT"
                     /locus_tag="ACHJ12_000204"
     CDS             complement(75743..77233)
                     /gene="nanT"
                     /locus_tag="ACHJ12_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4988055.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77342..78235)
                     /gene="nanA"
                     /locus_tag="ACHJ12_000205"
     CDS             complement(77342..78235)
                     /gene="nanA"
                     /locus_tag="ACHJ12_000205"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4988056.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78357..79148)
                     /gene="nanR"
                     /locus_tag="ACHJ12_000206"
     CDS             complement(78357..79148)
                     /gene="nanR"
                     /locus_tag="ACHJ12_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4988057.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            79528..80895
                     /gene="dcuC"
                     /locus_tag="ACHJ12_000207"
     CDS             79528..80895
                     /gene="dcuC"
                     /locus_tag="ACHJ12_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4988058.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(80938..81435)
                     /gene="sspB"
                     /locus_tag="ACHJ12_000208"
     CDS             complement(80938..81435)
                     /gene="sspB"
                     /locus_tag="ACHJ12_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4988059.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(81441..82079)
                     /gene="sspA"
                     /locus_tag="ACHJ12_000209"
     CDS             complement(81441..82079)
                     /gene="sspA"
                     /locus_tag="ACHJ12_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4988060.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(82474..82866)
                     /gene="rpsI"
                     /locus_tag="ACHJ12_000210"
     CDS             complement(82474..82866)
                     /gene="rpsI"
                     /locus_tag="ACHJ12_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4988061.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(82882..83310)
                     /gene="rplM"
                     /locus_tag="ACHJ12_000211"
     CDS             complement(82882..83310)
                     /gene="rplM"
                     /locus_tag="ACHJ12_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4988062.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(83529..84656)
                     /gene="zapE"
                     /locus_tag="ACHJ12_000212"
     CDS             complement(83529..84656)
                     /gene="zapE"
                     /locus_tag="ACHJ12_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4988063.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            84850..85248
                     /gene="zapG"
                     /locus_tag="ACHJ12_000213"
     CDS             84850..85248
                     /gene="zapG"
                     /locus_tag="ACHJ12_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4988064.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            85402..86769
                     /gene="degQ"
                     /locus_tag="ACHJ12_000214"
     CDS             85402..86769
                     /gene="degQ"
                     /locus_tag="ACHJ12_000214"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4988065.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            86859..87926
                     /gene="degS"
                     /locus_tag="ACHJ12_000215"
     CDS             86859..87926
                     /gene="degS"
                     /locus_tag="ACHJ12_000215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4988066.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(87988..88926)
                     /gene="mdh"
                     /locus_tag="ACHJ12_000216"
     CDS             complement(87988..88926)
                     /gene="mdh"
                     /locus_tag="ACHJ12_000216"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4988067.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            89361..89831
                     /gene="argR"
                     /locus_tag="ACHJ12_000217"
     CDS             89361..89831
                     /gene="argR"
                     /locus_tag="ACHJ12_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4988068.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            90196..90459
                     /gene="yhcN"
                     /locus_tag="ACHJ12_000218"
     CDS             90196..90459
                     /gene="yhcN"
                     /locus_tag="ACHJ12_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4988069.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(90515..90787)
                     /gene="yhcO"
                     /locus_tag="ACHJ12_000219"
     CDS             complement(90515..90787)
                     /gene="yhcO"
                     /locus_tag="ACHJ12_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4988070.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(90879..92846)
                     /gene="aaeB"
                     /locus_tag="ACHJ12_000220"
     CDS             complement(90879..92846)
                     /gene="aaeB"
                     /locus_tag="ACHJ12_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4988071.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEKLEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(92852..93784)
                     /gene="aaeA"
                     /locus_tag="ACHJ12_000221"
     CDS             complement(92852..93784)
                     /gene="aaeA"
                     /locus_tag="ACHJ12_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4988072.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(93792..93995)
                     /gene="aaeX"
                     /locus_tag="ACHJ12_000222"
     CDS             complement(93792..93995)
                     /gene="aaeX"
                     /locus_tag="ACHJ12_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4988073.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            94178..95107
                     /gene="aaeR"
                     /locus_tag="ACHJ12_000223"
     CDS             94178..95107
                     /gene="aaeR"
                     /locus_tag="ACHJ12_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4988074.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(95235..96680)
                     /gene="tldD"
                     /locus_tag="ACHJ12_000224"
     CDS             complement(95235..96680)
                     /gene="tldD"
                     /locus_tag="ACHJ12_000224"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4988075.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(96836..100636)
                     /gene="yhdP"
                     /locus_tag="ACHJ12_000225"
     CDS             complement(96836..100636)
                     /gene="yhdP"
                     /locus_tag="ACHJ12_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4988076.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(100704..102173)
                     /gene="rng"
                     /locus_tag="ACHJ12_000226"
     CDS             complement(100704..102173)
                     /gene="rng"
                     /locus_tag="ACHJ12_000226"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4988077.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(102163..102756)
                     /gene="yhdE"
                     /locus_tag="ACHJ12_000227"
     CDS             complement(102163..102756)
                     /gene="yhdE"
                     /locus_tag="ACHJ12_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4988078.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(102765..103253)
                     /gene="mreD"
                     /locus_tag="ACHJ12_000228"
     CDS             complement(102765..103253)
                     /gene="mreD"
                     /locus_tag="ACHJ12_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4988079.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(103253..104356)
                     /gene="mreC"
                     /locus_tag="ACHJ12_000229"
     CDS             complement(103253..104356)
                     /gene="mreC"
                     /locus_tag="ACHJ12_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4988080.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(104422..105465)
                     /gene="mreB"
                     /locus_tag="ACHJ12_000230"
     CDS             complement(104422..105465)
                     /gene="mreB"
                     /locus_tag="ACHJ12_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4988081.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(105770..107710)
                     /gene="csrD"
                     /locus_tag="ACHJ12_000231"
     CDS             complement(105770..107710)
                     /gene="csrD"
                     /locus_tag="ACHJ12_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4988082.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            107862..108836
                     /gene="yhdH"
                     /locus_tag="ACHJ12_000232"
     CDS             107862..108836
                     /gene="yhdH"
                     /locus_tag="ACHJ12_000232"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4988083.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            109814..110284
                     /gene="accB"
                     /locus_tag="ACHJ12_000233"
     CDS             109814..110284
                     /gene="accB"
                     /locus_tag="ACHJ12_000233"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4988084.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            110295..111644
                     /gene="accC"
                     /locus_tag="ACHJ12_000234"
     CDS             110295..111644
                     /gene="accC"
                     /locus_tag="ACHJ12_000234"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4988085.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            111753..111995
                     /gene="yhdT"
                     /locus_tag="ACHJ12_000235"
     CDS             111753..111995
                     /gene="yhdT"
                     /locus_tag="ACHJ12_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4988086.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            111985..113436
                     /gene="panF"
                     /locus_tag="ACHJ12_000236"
     CDS             111985..113436
                     /gene="panF"
                     /locus_tag="ACHJ12_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4988087.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            113448..114329
                     /gene="prmA"
                     /locus_tag="ACHJ12_000237"
     CDS             113448..114329
                     /gene="prmA"
                     /locus_tag="ACHJ12_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4988088.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            114658..115623
                     /gene="dusB"
                     /locus_tag="ACHJ12_000238"
     CDS             114658..115623
                     /gene="dusB"
                     /locus_tag="ACHJ12_000238"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4988089.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            115649..115945
                     /gene="fis"
                     /locus_tag="ACHJ12_000239"
     CDS             115649..115945
                     /gene="fis"
                     /locus_tag="ACHJ12_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4988090.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            116031..116915
                     /gene="yhdJ"
                     /locus_tag="ACHJ12_000240"
     CDS             116031..116915
                     /gene="yhdJ"
                     /locus_tag="ACHJ12_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4988091.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            116999..117178
                     /gene="yhdU"
                     /locus_tag="ACHJ12_000241"
     CDS             116999..117178
                     /gene="yhdU"
                     /locus_tag="ACHJ12_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4988092.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(117181..117843)
                     /gene="acrS"
                     /locus_tag="ACHJ12_000242"
     CDS             complement(117181..117843)
                     /gene="acrS"
                     /locus_tag="ACHJ12_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4988093.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT        29008 a        31547 c        29330 g        28088 t
ORIGIN      
        1 ataaggttat aaatgaataa agttacaaaa acagctattg tgggcttact tgcgcttttc
       61 gcaggtaatg ccgctgcaac cgatggtgaa atagtttttg atggcgagat tttaaaatct
      121 gcttgtgaaa tcaatgactc tgataagaaa attgaagttg ctcttggtca ctataatgcc
      181 gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc cattcactat tcctttagtt
      241 aactgcccag tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc tttccgttta
      301 tggctggaaa cgcgggataa tggcaccgtc cctaacttcc ctaatctggc aaaagtgggc
      361 tcttttgctg gcacagcggc aacaggtgtg ggtatccgta ttgacgatgc ggaaagcgga
      421 aaccttatgc cactgaatgc tatggggaat gataacacgg tttatcagat cccagcggac
      481 tccaatggta ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt tgaagcatca
      541 gaaatcaccc caggggaagc agacgctgtc gttaacgtaa cgctggatta ccgttaatac
      601 gttacggcgt tatctgacct ttcagataac gcccttttcc ttcctctttc tcgttgtatc
      661 aggttgaaaa ataactatgt caaaacgaac attcgcggtg ataataacct tgttgtgtag
      721 cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta
      781 tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag aaacgcctta
      841 tttactgcaa tcatgggtag ataatataga tggtacaagc cgtgccccat ttattataac
      901 cccaccgcta tttcgtcttg aagctggcga tgactcatca ctgcgaatta ttaaaacagc
      961 tgataacctg cctgaaaata aagagtcgct gttctacatt aatgttcgtg ccattccagc
     1021 aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg ctggtattta aaacacggat
     1081 caaaatgttt tatcgccccg cacacctgaa gggacgggta aacgatgcgt ggacatcact
     1141 ggaatttaaa cgtagtgacc attcactcaa tatatataac ccaactgaat attacgtcgt
     1201 atttgccgga ctggcagtcg ataaaaccga tctcacaagc aaaattgaat atatcgcgcc
     1261 cggagaacat aaacagttac cacttcctgc atctggcgga aagaacgtga aatgggctgc
     1321 aatcaatgat tatggcggca gttccgggac agaaactcgt ccactgcaat aacaaatata
     1381 aaaaacacag gtcatcaggg aatgccacaa cgacaccacc agggacataa acgcacaccg
     1441 aaacagttgg cgctcatcat caaacgctgt ttgccgatgg tgctcactgg cagcggcatg
     1501 ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct ggaaactaca
     1561 aaaagtggta tgcaaacaac tgatctgtca cgtttttcaa aaaaatacgc acaactacca
     1621 ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa aaaaattaca
     1681 tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca actacgtgag
     1741 ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga tagcgtgatc
     1801 aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa tcatcagcga
     1861 cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta cgtctcccct
     1921 tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac aggttctgat
     1981 aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca aaatggtgct
     2041 aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga tcaggcgtca
     2101 agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa gtctcagttg
     2161 cttctgggag aaagcgccac cagcggcagt attttttcca gctacacctt tactggcgtg
     2221 caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc cccaacggta
     2281 cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta tgtgatctat
     2341 caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc ttccaacagc
     2401 ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt tatccagcct
     2461 tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc gaccgctgga
     2521 cgctatcgcg ctgatgcaaa cagtgatagc aaggaaccag aatttgctga agccacggca
     2581 atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc tgaagattat
     2641 tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt gtcgatggat
     2701 atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta tcaatggcgt
     2761 acgcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag ctactatcgc
     2821 tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg ggactataac
     2881 agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga tggggtaagt
     2941 ctgtatgcct ccggttcgca gcaagactat tggggcaata acgagaaaaa caggaatatc
     3001 tctgttgggg tttccggcca gcaatgggga attggttaca gcctgaatta tcaatacagc
     3061 cgctacactg atgaaaataa tgaccgtgca ctctctttga atctcagtat tccgttagaa
     3121 cgctggttac cgcgtagccg ggtttcctat cagatgacca gccagaaaga tcgcccaacc
     3181 caacatgaaa tgcgtcttga tggctcactg ctggatgatg gtcgcctgag ctatagcctg
     3241 gaacaaagtc tggatgacga taacaaccat aacagtagcc tgaacgccag ttaccgttca
     3301 ccttatggca ccttcagtgc cggatacagc tacggtaatg acagcagcca atacaattac
     3361 ggcgttaccg gcggcgtggt tatccatcct catggcgtga cgctctcgca atatctgggc
     3421 aacgcttttg cgcttatcga tgctaatggg gcttctggcg tgaggataca aaactatccg
     3481 gggattgcta ctgatccctt tggctatgca gtggttcctt atctcacgac ttaccaggaa
     3541 aaccgtctct cggtagatac tacgcagctg cccgataacg tcgatcttga acaaacaaca
     3601 cagtttgtgg tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc caatatcggt
     3661 tatcgcgtac ttgttacagt cagcgatcgc aacggtaaac cgttgccctt tggcgctctt
     3721 gccagcaacg atgagacggg gcaacaaagt atcgtcgatg agggcggcat actatatctc
     3781 tctgggatat cgagtaaatc acaaagctgg actgtacgct ggggaaatca ggcagatcaa
     3841 caatgtcagt ttgcttttag tacaccggat tcagaaccaa caacctctgt attacaaggc
     3901 acggcgcagt gccattaagg ataaaaaaat gaaaagagcg cctcttataa caggactttt
     3961 gttgatatcc acatcctgcg cttatgcctc ctcaggaggg tgtggagctg acagcactag
     4021 cggtgcgaca aattacagca gtgtggttga tgatgttacg gtgaaccaga cagataacgt
     4081 gacaggacgg gagtttacct ctgcaacgct aagtagcact aactggcaat acgcctgttc
     4141 ctgctctgcg ggtaaggcag ttaaacttgt ctatatggtc agccccgtac ttaccaccac
     4201 tggacatcag acaggatatt acaaactcaa tgacagcctg gatattaaaa ccacattaca
     4261 ggcaaacgac attccaggac tcacaaccga ccaggttgtc tctgttaaca cccgattcac
     4321 acagataaaa aacaacacgg tatattctgc tgcaacccaa acgggtgttt gccagggtga
     4381 tacgtctcgt tatggacccg ttaatattgg tgcgaacacc acctttaccc tgtatgtcac
     4441 caagccattt ctcggctcga tgaccattcc gaaaacggat attgccgtca ttaaaggcgc
     4501 gtgggtcgat ggaatgggaa gcccgtctac aggtgacttc catgatttag tcaagttatc
     4561 gattcaggga aatctcaccg ccccacagtc gtgcaaaatt aatcagggcg atgttattaa
     4621 ggttaatttt ggattcatca atggtcagaa gtttaccacc cgcaatgcca tgccagacgg
     4681 ttttactcca gtagactttg atatcactta tgactgtggt gatacttcaa agattaaaaa
     4741 ctcgttgcaa atgcgcatcg acggtacaac tggggtagta gaccagtaca acctggtcgc
     4801 caggcgaaga agttcagaca atgtgcccga tgtcggtatt cgtattgaaa atctcggcgg
     4861 cggagttgca aatattcctt ttcagaacgg tatccttccc gttgatcctt ccgggcatgg
     4921 caccgtcaat atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa
     4981 atttcagggc acagccacca ttaccgtcat cgtgcggtaa aagaagacgt tgcgttttga
     5041 ttaacgcaac gcttcggcac tgttacccct gctgctccag cgcatactta tacagcgcat
     5101 ttttcttcac gccgtgaatt tctgcggcca gcgccgccgc ttttttcagc ggcagttctg
     5161 cctgtagcag cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc tgtgctttat
     5221 gaccttcgac aatcagcacc atttcgcctt tgcgacggtt ttcatcttcc tttacccacg
     5281 ccagcagctc gccaacgggc gcgccgtgaa tggtttccca ggttttggtc agctcacgcg
     5341 ccagaaccac gtagcgggat tcgcctaata ccgcaacgat atcttccagg ctatctaaca
     5401 gacggtgggt agattcataa aaaatcagcg tgcgcggctc cgcttcaatg gcttttagcg
     5461 catcacggcg gccttttgat ttggcaggta aaaagccttc gtaacagaaa cggtcagagg
     5521 gtaaacccgc tgcgcttaac gcagtgatag cagcacacgg cccgggtagc ggcaccacgc
     5581 ggatccccgc ttcacggcag gtacgcacca gatggtagcc aggatcgtta attagcggcg
     5641 ttccggcatc ggaaaccagc gcaatgttct gcccttcttg cagcttcgcc agcagcgttt
     5701 cggctttttg ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat
     5761 gttgcagcaa taaaccggtg tgacgagtat cctcggcggc aatcagatca acggcctgta
     5821 atacctctaa cgcacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt
     5881 aaagctggcc ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc
     5941 cgatttaata ttgagcattg cgtaaaaaaa tatcactgga tacattatgg taccctcaac
     6001 attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc
     6061 cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca cggcgcaggc
     6121 tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca ggatcaactg
     6181 gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg cggttgagtt
     6241 gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa cactgctggc
     6301 ggtagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc tggcgaaaat
     6361 cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc
     6421 cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg aaccgctgct
     6481 cggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct cctccatgac
     6541 tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc aaggctggct
     6601 ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga aagccgggat
     6661 cgccgactgg caaaaacgtt atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt
     6721 ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc tgttgccgct
     6781 gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag cggcgaaaaa
     6841 tatcggcact cagccagtgg cggctcaggt agctgccgca cctgccgcag acgtagctga
     6901 acaacctcag ccgcaaactg cggatggcgt tgccagcccg gcacaagcct cggttagcga
     6961 tctgaccggt gaacagcctg cggcacagcc agcgcctgta agcaccccgg cagcaaccac
     7021 cacagcggta agcgcgccag cgaatccatc cgcagaactg aaaatctacg atacctcatc
     7081 acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta ttgtggttgg
     7141 tccgttgctg aaaaataacg ttgaagagtt gcttaagagc aacaccccgc tgaacgtgct
     7201 ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc
     7261 accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta aacaagcgcc
     7321 gctggtgctg atcccacgca gttcattggg cgatcgcgta gccaatgcgt ttgcgcaaga
     7381 gtggcagaaa ctaggcggcg gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt
     7441 acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga ttactcccag
     7501 agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca cgccaaccga
     7561 tgaccagttc accaataatg gcggtcgtgt cgatgcggtg tacattgtgg caacgccggg
     7621 tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac
     7681 gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc ccggatttcc gactggagat
     7741 ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctac cgttaatgca
     7801 gcaggcactc agcgcggtga ataacgatta ttcactggct cgcatgtatg cgatgggcgt
     7861 cgatgcctgg tcgctggcaa atcatttctc acaaatgcgc caggttcagg gttttgaaat
     7921 caacggtaat accggaagcc tgacggcaaa cccggattgc gtgattaaca ggaagttatc
     7981 atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa acagaccggc
     8041 gatgcgtggg aagtacaagc gcgtcgctgg ctggaaggca aaggactgcg atttgtcgcc
     8101 gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg ccggaccacc
     8161 gtttttgtcg aggtacgcta tcgccgctct gcgctttatg gcggcgcggc agccagtgtg
     8221 acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat
     8281 gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt
     8341 gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca
     8401 agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc
     8461 gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa
     8521 caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag
     8581 catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga
     8641 taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca
     8701 ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag
     8761 ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt
     8821 gaccggctat gacggcggcg agcttgcagg tttgttaggg ccacaggatg tggagatccg
     8881 cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct
     8941 gtgcgatctg atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa
     9001 ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc
     9061 tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg
     9121 cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga
     9181 acagattaag aaagaaaccc gtattaatgt cacggcgtat cagggcaaag tgctgctggt
     9241 tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga
     9301 cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc
     9361 atctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt
     9421 gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt
     9481 gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg
     9541 ggtaaccaca gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct
     9601 tgccgcgtct tatcaggcat acaacgatgc aaaccgtaga tcggataagg cgtttacgcc
     9661 gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc
     9721 agacactgcc accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag
     9781 cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc
     9841 ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat
     9901 gacaaacaag catcctgcca ccgccatcgc caccacccac atcaggctgt tatcgacagc
     9961 accatcggca tgggggaata accagacgcg agcggcaccc aacaccagta ctgcaaggat
    10021 gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc ctgccccagc ggctaaaaaa
    10081 accgccctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg
    10141 cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg
    10201 aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa
    10261 caccggattg cccatccaga atgccagcgc accgcccatc gacacctgtt ggcgacgcat
    10321 tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt
    10381 tcccagcagc gtgctgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg
    10441 gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt tccataccgc
    10501 gaggaaatag atcatcgcgt aatccaacgc cgcctgccat gggttagcat ccgcctgcgc
    10561 aaggatagat ttaccgatac tgtgggtttc ggcagcagta aacgctttgc cgtagtaagg
    10621 ttcccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca
    10681 ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa
    10741 taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta
    10801 ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg
    10861 catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc
    10921 tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc
    10981 gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg cgattaatgc
    11041 ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat
    11101 cgcactgact accaacatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac
    11161 taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt
    11221 gccgagacaa caaaacacaa tgtcgatggg atcggtgacc tgcgccaacg cgtcactcag
    11281 ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc
    11341 agcaatggcg ttaactttcg gttcgttaat caacatccgc agcaggtgac cgcccaccag
    11401 ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc
    11461 ttgcgctcca cggctcatgc accaggctta atagaccatg aggtaattat ccccgattgt
    11521 gggaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt
    11581 tttaatcact gatgaatttg aggattcaga atttacttca cccgcagacg agttccgtaa
    11641 agccggacac gaagtgatta ccattgaaaa acaagcgggt aaaacggtga aaggcaaaaa
    11701 aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgcctg cggagtttga
    11761 tgccctgctg ctaccgggcg gccattcacc ggattatctg cgtggggaca accgttttgt
    11821 cacctttacc cgtgattttg tgaatagtgg caaaccggtg tttgccatct gtcacggccc
    11881 gcagttgctg atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat
    11941 cattattgat gttaaaaatg cgggcgcgga attttacgat caggaagtcg tggttgataa
    12001 agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt
    12061 acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt
    12121 tgtcggtgaa tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg
    12181 caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc
    12241 tgcggatctc acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt
    12301 ttaccgttcc ggcaactgcc gcctgcggcc cgctcatctg cgcgtggcgc gttttttctt
    12361 ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc
    12421 agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc
    12481 tgatggcggt gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat
    12541 gcgatgcaga cgatgacacc ctggtttctt tacttgatcc gtaccgccga caataagctt
    12601 tataccggga tcaccacgga tgtcgaacgc cgctatcagc aacatcaaag cggcaaaggg
    12661 gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgat
    12721 cgttcgctgg cgttacgggc agaatatcgc gttaagcaac tgaccaaacg gcaaaaagag
    12781 cgtctggtag cggaaggcgc agggtttgca gagctgttga gcagcctgca aaccccaaag
    12841 attaaaagcg attgaaatgc tcgtgatact caaccaggcc ggtaacgcca ttcagcgcgt
    12901 catccgctaa acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat
    12961 cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca
    13021 ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg
    13081 ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca
    13141 ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt
    13201 catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct
    13261 tcgcttccgc atcactttcg aatgagcgac gtagcagggc atcaataccc ggcgcatcaa
    13321 tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc
    13381 ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt
    13441 cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacagcc ccagctccgt
    13501 atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc
    13561 cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg
    13621 gctgaccacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac
    13681 atgaatactt aaccagcggc cttcaagaaa ctccagctca ccatcatcca gcgcctgacg
    13741 gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt
    13801 taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa
    13861 cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat
    13921 agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg
    13981 ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg
    14041 cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct
    14101 cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga
    14161 tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc
    14221 ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca
    14281 tccggacggt tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc tgggtgccga
    14341 cgcgctgatc ctcgccgacc tcgccatgct ggaatacgcc gccgagcgtt atccgcatat
    14401 tgagcgccac gtatcggtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca
    14461 tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa
    14521 acaactggca cgggtcacac ctgtaccact ggaagtcttt gctttcggca gcctgtgcat
    14581 tatgtcggaa ggtcgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacaccgt
    14641 aggcgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc
    14701 ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac
    14761 gctatgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac
    14821 cagtctcaat accctggaac tgctgccgga gttaatggcg gcgaatattg cttcggtgaa
    14881 aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca
    14941 ggctatcgac cgttgtaagg ccgatccgca aaacttcata ccgcaaagcg cgtggatgga
    15001 gacgctcggg tcgatgtccg aaggcacgca gaccaccctt ggcgcgtatc accgtaaatg
    15061 gcagtgagaa gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag
    15121 acgctggaag atttttatca gcaggccgcc accagcagcg ccgacgtgat ttatcttggt
    15181 gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg actggctgga gatggcaaaa
    15241 tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc
    15301 tctgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc
    15361 gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg
    15421 ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg
    15481 tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag
    15541 ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc
    15601 tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc
    15661 tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt
    15721 gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca
    15781 tccatgcagg ggctggttga tgtggtccgc ctgtcaccgc agggtactga cactttcgcg
    15841 atgctcgacg ccttccgcgc taatgaaaat ggcgctgcgc cactattgtt gacggcaaac
    15901 agcgactgta acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat
    15961 atgctcgtca tacttcaagt tgcatgtgct gcatctgcat tcgttcaccc cagtcactta
    16021 cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg aattatttag
    16081 agcacagctc actttgttaa caactttaac tactctttaa tgcagtatta aagattaatc
    16141 ggtaacaaag tgagctgtta tgactgataa aaccattgcg ttttcgctac tcgatctggc
    16201 ccccattccc gaaggttctt cagcgcgaga agcattctcc cactctctcg atctcgcccg
    16261 tctggctgaa aagcgcggct atcatcgcta ctggctggca gaacaccaca atatgactgg
    16321 cattgccagt gctgccacgt cggtattgat tggctatctg gcggcgaata ccaccacgct
    16381 gcatctgggg tctggcggcg tgatgttgcc taaccactca ccgttggtca ttgccgaaca
    16441 gttcggcacg ctcaatacac tctatccggg gcgaatcgat ttggggctgg gtcgtgcgcc
    16501 aggtagtgac caacggacaa tgatggcgct gcgtcgccat atgagcggcg atattgataa
    16561 tttcccccgc gatgtcgcgg agctggtgga ctggtttgac gcccgcgatc ccaatccgca
    16621 tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg tggttgttag gctccagcct
    16681 ttacagcgcg caactggcgg cgcagcttgg tctgccgttc gcgtttgcct cacacttcgc
    16741 gccggatatg ttgttccagg cgctgcatct ttatcgcagc aacttcaaac cgtcggcacg
    16801 actggaaaaa ccatacgcga tggtgtgcat caatattatc gccgccgata gcaaccgcga
    16861 cgctgagttt ctgtttacct caatgcagca agcctttgtg aagttgcgcc gtggcgaaac
    16921 cgggcaactg ccgccaccga ttcaaaatat ggatcagttc tggtcgccgt ccgaacagta
    16981 tggtgtgcag caggcgctga gtatgtcgtt ggtaggtgat aaagcgaaag tgcgtcatgg
    17041 cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt atggtcaacg ggcagatttt
    17101 cgaccaccag gcgcggctgc attcgtttga gctggcgatg gatgttaagg aagagttgtt
    17161 gggatagtgt gtcttaacgc gggaagcctt atccgagctg gcaacgctgt cctacgtaga
    17221 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
    17281 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
    17341 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
    17401 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
    17461 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
    17521 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
    17581 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
    17641 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
    17701 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
    17761 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
    17821 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
    17881 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
    17941 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
    18001 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
    18061 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
    18121 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
    18181 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
    18241 aggcaatgga aatgccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatag
    18301 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
    18361 gcagcataca gaaccaggtg aagatcagcg ccgccattga ccagaagaac cacgccccgg
    18421 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
    18481 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
    18541 tccagtgaaa aatagtgcga ctgcgttgtt atgtattgca ccgtaccagt acacgagtac
    18601 aaaagacaga aaaaaagccc cgatggttca aatcggggct gtatatatta ttttacagac
    18661 tgtgttcgct gttcagcgat gattacgcat cgccaccgaa acgacgacga ccggtagagt
    18721 catcacgacg cggtgcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
    18781 gaccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgc tcgccaccga
    18841 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
    18901 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
    18961 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
    19021 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
    19081 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
    19141 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
    19201 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
    19261 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
    19321 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
    19381 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
    19441 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
    19501 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
    19561 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
    19621 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
    19681 tgtccagacg accatctttc aggcgttcca gtgtctgctc acgcagcgcc tggttcatgt
    19741 caccgttcag cgcggcactg ttgtagccgt tacgctcaag agcttcagcc acttccagag
    19801 tcgcattttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
    19861 gtaccagtgc ttcgttttta cgcatacccc agacagtcca gtagctctgg ctgatgtcag
    19921 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
    19981 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
    20041 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
    20101 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
    20161 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
    20221 gcacgtcata acgctggccg ccgtacagag caaccacatt tacgccgcgc atgtgtttag
    20281 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
    20341 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
    20401 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
    20461 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
    20521 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
    20581 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
    20641 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
    20701 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
    20761 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
    20821 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
    20881 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
    20941 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
    21001 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
    21061 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
    21121 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
    21181 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
    21241 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcggcatc
    21301 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
    21361 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
    21421 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
    21481 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
    21541 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
    21601 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
    21661 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
    21721 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
    21781 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
    21841 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
    21901 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
    21961 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
    22021 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
    22081 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
    22141 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tatccgggtt
    22201 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
    22261 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
    22321 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcagtg cagagatacc
    22381 gtcgcgggaa cctgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
    22441 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
    22501 ggcagctttg attggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
    22561 catagaagaa gaaccgttgg attcggtgat ttcagacact acacgtacgg tgtacgggaa
    22621 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
    22681 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
    22741 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
    22801 acgagcggta cccagcgttg cagtgaccag tgcctgcgtt tcaccgcggg tgaacagcgc
    22861 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
    22921 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
    22981 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
    23041 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
    23101 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
    23161 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
    23221 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
    23281 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
    23341 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
    23401 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
    23461 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
    23521 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
    23581 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
    23641 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
    23701 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
    23761 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
    23821 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
    23881 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
    23941 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
    24001 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
    24061 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
    24121 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
    24181 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
    24241 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
    24301 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
    24361 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
    24421 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
    24481 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
    24541 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
    24601 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
    24661 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
    24721 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
    24781 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
    24841 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
    24901 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
    24961 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
    25021 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
    25081 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
    25141 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
    25201 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
    25261 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
    25321 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
    25381 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
    25441 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt actcctcctt
    25501 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
    25561 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
    25621 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
    25681 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
    25741 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
    25801 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
    25861 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
    25921 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
    25981 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
    26041 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
    26101 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
    26161 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
    26221 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
    26281 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
    26341 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
    26401 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
    26461 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
    26521 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
    26581 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
    26641 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
    26701 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
    26761 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
    26821 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
    26881 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
    26941 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
    27001 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
    27061 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
    27121 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
    27181 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    27241 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    27301 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    27361 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    27421 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    27481 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    27541 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    27601 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    27661 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    27721 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    27781 tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg aagccttgct
    27841 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    27901 cacgatcagc ttttgattca gcgtgtttgt tacctttctt cggacgcgct gctttcgcgt
    27961 tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttcg gcctggcgag
    28021 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    28081 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    28141 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    28201 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    28261 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    28321 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    28381 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    28441 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    28501 caccggtacc aggaatgtta agggtgctgc gtgttttacg ctgcagcgtc aatttgtccg
    28561 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    28621 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    28681 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    28741 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    28801 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    28861 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    28921 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    28981 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    29041 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    29101 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    29161 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    29221 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    29281 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    29341 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    29401 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    29461 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    29521 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    29581 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    29641 aacgagtgac gaacagttgc gcgccacgcg cttccgggtg aacggaatag agcacgccac
    29701 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    29761 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    29821 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    29881 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    29941 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    30001 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    30061 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    30121 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    30181 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    30241 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    30301 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    30361 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    30421 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    30481 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    30541 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    30601 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    30661 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    30721 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    30781 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    30841 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    30901 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    30961 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    31021 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    31081 aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat tgctaatcat
    31141 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctccccc
    31201 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    31261 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    31321 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    31381 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    31441 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    31501 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    31561 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    31621 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    31681 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    31741 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    31801 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    31861 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    31921 ctccacagac tccaacatgc ttggtgcaac gcacgaagcg aaggatctgg aatacctcaa
    31981 ctccagcgtc aaaatcgtca acccaattat gggcgtgaaa ttctgggatg agagcgtgaa
    32041 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtag cgctgaactg
    32101 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    32161 cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa gccgtggtat
    32221 ttacgaggct ccggggatgg cactgctaca tatcgcttat gaacgtctgt tgaccggtat
    32281 tcacaacgaa gacaccattg agcagtatca tgcacatggt cgtcagttgg gccgtctgct
    32341 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    32401 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    32461 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    32521 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    32581 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    32641 tgccacttca ggcgtgccgc aggtggagaa tctggaaaac aaagttaagt aatatcatag
    32701 ctttcgtgct aaaggccgca aatgcggcct ttttttacgg ctaagagaaa gtgactgcgt
    32761 ttaggggcca ccatgtccga attcggaatt aaagtaaaag ttaaatgaaa taaagtaaat
    32821 ttcagcgcat tctattttta ctttatgttt ttatgctcag taaaaggaat gaccgatgaa
    32881 tggtgaatat tttgtacgcc tcgcattaca tactctgaaa tgcactcaaa aagatttagc
    32941 aagccactta ggtgtttcct caacacaaat tagcaagtgg aaaaaagggg aacacatgtc
    33001 cgctgatatg gaaaaaaagt tcagaaatat tacacaaata ggagattatt ctcctcaact
    33061 agttgaatgg acgggctcag tcgcgaatgc tgagaaatgg gatcgactta tccattttct
    33121 tgctcgacga gctatggacg atgctgaaac gggttatata acacaacttc ttactgacga
    33181 agatggtttt ttggttgaag aaacaattga tgtattaaag cgaataggtt ttcctacacc
    33241 tctttccttt cctgaaggac ttaatattga cgacgataat gctgatgaag aagaggcttt
    33301 ttgggagata ttagagagca atgctcattg ttctgtaatt aatgatattt atcatgctct
    33361 gaatgatgtt tatggtttct acatagcata tgttgatgag ttgattcagg acgacgattt
    33421 agatgtctat tcaagcgaag ccatcaatat tcagagttca ttgatatcac ttgctgcatg
    33481 caaaattgaa attgatactc ctgttgcgtc aaactttaaa gaatttcgtt atagagttaa
    33541 aaaagactac gaaaattggt tgaatcaact caagatgatg gcattcaggg cgggaattcc
    33601 gttaagagcc gaacttctgg aaatggttta taacaccgct gaccaactta gcgttgcagc
    33661 agaagccgaa cgtttcgatt tcaataaatc tcgcattcat ccagatatat acatgaacga
    33721 gatccttact ggcatgagaa taattcacca agtgcttccg gtaataatgc aaaagttaga
    33781 aattacagat ttcaaactag atgaaacaga tttatgcctg ggtaaatagt tatcaaatta
    33841 agaatactta gcctggctta taaaacgaaa aaagacgctt ttcagcgtct cttttctgga
    33901 atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg
    33961 tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt cgggatatcg
    34021 ctggtcggct tagccggagc agctggttga gtttgttcgg ttttcaccgg tgcactcaga
    34081 ttttcccatt cgctaccttt attggttttg ttgctattga tgttacccag caccagactg
    34141 atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt accagaacca
    34201 cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct
    34261 ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag gaaaactact
    34321 aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca
    34381 accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata ctaaccaaag
    34441 cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa aaaacagcaa
    34501 aaccgatgtg ttcgctgaga aaaaaggcga caatcgccgc ctttttagcc agttatctaa
    34561 cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt
    34621 cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta cgcagcaaca
    34681 cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta actgactcat
    34741 gctcaggtgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca
    34801 ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc gccgccagca
    34861 cctgcaagcc agcaacgatg ccgtcaccgg tagtagtttt atccagcagg atcacatgac
    34921 cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt
    34981 cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt tcaagcccca
    35041 tgttgctcat caatgtaccc acagcgccac cacgcagctg gccctgacga agaccttcac
    35101 gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg tcaaccataa
    35161 tcacgcgatc gccatcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac
    35221 gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt acaccgtttg
    35281 gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt
    35341 gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg ctaagttcgt
    35401 tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg atacggctgg
    35461 ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg
    35521 cctcttctac cgcatccggc agtttggtgc cgtcgataga gaagaattta atcccattat
    35581 cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg aaggtacgcg
    35641 tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg
    35701 ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac
    35761 caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc caacccagct
    35821 taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac
    35881 cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt ggcttccacc
    35941 acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc
    36001 attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc
    36061 agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg
    36121 tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg
    36181 aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt
    36241 tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg agcttcctgc
    36301 atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc
    36361 gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg agcgccaact
    36421 ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca gacttcgaag
    36481 cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc aacgctaact
    36541 tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg
    36601 atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc accatccgaa
    36661 aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa
    36721 gtaccctggg caaagagttt catgatgtta tccctggtat gaattgataa gaaaaaaccc
    36781 cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga acttacttgt
    36841 cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac
    36901 gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt tcttcccagc
    36961 ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag
    37021 tctcatattt catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa
    37081 gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac
    37141 gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg aatacttcac
    37201 cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac tgggtcacca
    37261 tgttacgtgc cagattggtc gcaactttaa tatcgttgga cgcaccggta gaaacatgtt
    37321 ccggcccata gatgatctct tctgccagac gaccaccgta cagcgtagaa atctggcttt
    37381 ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca
    37441 gcgcacgacc gcgtgggata atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc
    37501 gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc gcttccgtca
    37561 tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa
    37621 ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt
    37681 tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc gcgtcgatat
    37741 cgggggccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat
    37801 ctggcaagcc gaccacaacc tgacggtcga aacggccagg acgcagcagg gccgggtcga
    37861 gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct tcgaagccat
    37921 ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccagac
    37981 cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg
    38041 ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt
    38101 ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaatcgctt
    38161 tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct ttcgggatct
    38221 taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt tcagcaactt
    38281 cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg
    38341 tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg ccgccctgca
    38401 tttgacgcat gaagaagatc cagacaccaa ttagcaacag catcgggaac caggagatga
    38461 agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg acgttcttgg
    38521 tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac
    38581 tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt
    38641 tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc
    38701 caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt
    38761 tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga
    38821 tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc
    38881 cggtcgctac aatatacact tcccgcgaac gtgcacgaga agagtccggc ttacgaactt
    38941 tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga
    39001 acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt tctaacgcca
    39061 gttccaccag atacatggca cgggggatat ccaccgccgg tgttccgctc atgtttggtg
    39121 ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca
    39181 tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag
    39241 gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc acatattgtg
    39301 accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt
    39361 cactttgctg tatttcatca agtttaaacc aggcacggga acgtaacccc tttttctgtg
    39421 cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac
    39481 gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa
    39541 tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc
    39601 ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt
    39661 ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg
    39721 ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc
    39781 gaagatcgcg aaactaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt
    39841 aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga acgtaaaatc
    39901 tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa acgaggggtt
    39961 ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg
    40021 cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat
    40081 tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca
    40141 tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt
    40201 ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatccaga
    40261 ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca
    40321 tcaatcacct gcgcgttcga cagcttggct tcgatgtctt taatacggcc ttcgcagaaa
    40381 ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc
    40441 gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct
    40501 tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct
    40561 tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa
    40621 ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta
    40681 caattcacat tttgatagtc attttaccct gaagttcccg aagggtcatc gtttacttta
    40741 tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga
    40801 ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcgaat
    40861 gttgatgagt acgttactca actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa
    40921 gtcggcgcgt cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt
    40981 acgcagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc
    41041 accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg
    41101 gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact
    41161 ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc
    41221 gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct
    41281 ccgcctgccg ctgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca
    41341 aaacctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc
    41401 caggtacgca ccctcccccg aggttctgcc gaagcgcaat actgcgaact ggatgtcgta
    41461 ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc
    41521 ccgttggctt ttgccgtgca ggatggagcc agctatgccg gggcaattct gaaagatgag
    41581 ttaaaacagg caggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa
    41641 cctggaacgg tagttgccag taaacagtcg gccccgttgc acgatctgct taagattatg
    41701 ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatagg ccatgcgcgc
    41761 ttcaatgtgc ctggaacatg gcgggccggg tcggacgccg tgcgtcagat cctgcgccag
    41821 caagccggtg tcgatattgg aaacaccatt attgccgatg gttcagggct ttcgcggcat
    41881 aacctgattg cccccgccac catgatgcag gtgttgcaat acattgccca acacgacaat
    41941 gaacttaact ttatctccat gctgccgctg gcgggctatg acggctcttt gcagtaccgt
    42001 gcaggtctgc atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag
    42061 ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat ggcgtttgta
    42121 caatatcttt ctggctatgc agtagaaccg gcggatcagc gtaatcgccg tattccgtta
    42181 gtgcgttttg aaagccgttt gtataaagat atttatcaga acaattagtc aaaaagaaac
    42241 cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc
    42301 gtcgtcttcg tcccagtcat catcccagtc ctcatcatcc tcttcagcaa tctcttcaag
    42361 ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc
    42421 agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac
    42481 gcccagtcca ctcgccgcag agatcagata atatttatct tcccagccca gcgcttcagc
    42541 aatcgctttc gctttctctt cagcttctgc cttatccagc agatcgatct tattgaacac
    42601 taaccaacgt ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat
    42661 acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg atatcgatga ggtgcaacag
    42721 gacgcggcaa cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc cttccgcagc
    42781 gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac
    42841 cacacccaga cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc
    42901 cgataccgca cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc
    42961 agccagcagc atcagctcca gcagcagctc acgcttatcg cccggcgtac cgttagtttt
    43021 ctgccgtggt gtacggttaa ctgacgattt gaaacgggta ttgcccagac cgtgccagcc
    43081 gcccttagca accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccagt
    43141 accctggtcg attacacgcg tacctaccgg cactttaatt gtgacgtctt taccgcgctt
    43201 accggtacag tcgcggcttg cgccattctg accgcgctct gcacggaaag atttttcaaa
    43261 acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccaccatc
    43321 accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac
    43381 gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat
    43441 tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg
    43501 taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa
    43561 cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag
    43621 tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac
    43681 ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaaggtatt
    43741 cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg
    43801 ctttgccaga gggaagagcg caattgtaca taaagtgtac agtaaaaaca ggatctgcgg
    43861 tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc
    43921 cgcaccgacc ccaaagataa ctccccaggt gtaatcggtg agctttgtaa atatctcgac
    43981 cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg
    44041 agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc caactggcga
    44101 gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg agctgaacag
    44161 gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt
    44221 acgaaacacg cgtaatggcg gcaacctctt cttcaccgca agaatggcac caaggccaat
    44281 actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg
    44341 cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc
    44401 aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg
    44461 caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct
    44521 ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt
    44581 tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc
    44641 acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat
    44701 gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga
    44761 atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc
    44821 aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc
    44881 tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg
    44941 tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact
    45001 tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg
    45061 tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt
    45121 tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt
    45181 gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt
    45241 gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca
    45301 ttttttcagt acaatcactc tatattccta accataaacc ctaagttgcc tttgttcaca
    45361 gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt
    45421 taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg
    45481 tccagctgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc
    45541 cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca
    45601 ttgctgccct gatcgagttt atccacacgg cgactctgct gcacgacgac gttgtggatg
    45661 aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat gccgccagcg
    45721 tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcgc
    45781 tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc
    45841 aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata
    45901 gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta
    45961 cgccggaaga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt
    46021 tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg
    46081 acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac
    46141 cagaacaggc acagatgatc cgtaccgcca tcgaacaggg taacggtcgc catcttctgg
    46201 aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg
    46261 aggaagaagc agacaaagcc atcgcagcat tacaggtgct cccggacacc ccttggcgag
    46321 aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccttca
    46381 tcccgcgcag tgcgcgggat gattccagta aattccataa aaacactaat tcagcgctat
    46441 aacacctgaa aaaaagggcg atgaatcttt atatattctg aatattcaca ctctttacag
    46501 gaacttttta gagcaatagg ccatcaggag tatagtgatg cccgacagaa gaagtgttct
    46561 gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca
    46621 tcccgctgac atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg cggagtctcg
    46681 cagaaatggt ttgagttcct caacgctggc aaacgcatta tcgcgcccat ggccgaaagg
    46741 agagatgatt attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta
    46801 ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa
    46861 accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga
    46921 ttactcgcct ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg
    46981 ttcgtagcca cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca
    47041 gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga
    47101 aagtttttca acaccgtgac aaataacggt attgctttcg atttcggcat gcgcgcccat
    47161 acggctaagc tccggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc
    47221 ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc
    47281 cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct
    47341 aatccagtct tcgccgactt cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc
    47401 gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaaa tcgccgccgc
    47461 caccaggaaa gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa
    47521 acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag
    47581 cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat
    47641 cgtggtacct tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac
    47701 tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc
    47761 ttcttccagt ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg
    47821 acgcgcaccg atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac
    47881 cagcggcccc agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca
    47941 gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcccc
    48001 cagctggctt agcagcttca ttgatgtatc gacgtctttc agttttggga cgttctggat
    48061 ctctaccggt tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc
    48121 gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat
    48181 ttagtttgtt ctcagttaac aattcatatc cgctaccggc taatcgccca tagctcaaaa
    48241 gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc
    48301 atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt
    48361 aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc
    48421 gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt
    48481 attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt
    48541 tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag
    48601 gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag
    48661 aacatcagca ggcaaattat acaatttcgc gagggtatac actttgtcgt ttaccccctg
    48721 aagcgtcacg ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag
    48781 tccacccgta tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc
    48841 ttcctcacgc atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc
    48901 cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga
    48961 gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta
    49021 cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc
    49081 atgtcgtaag cctgccagtt tcccgtctgg gagtttttac gccactggaa gtccagacgc
    49141 accggcggac ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatcg
    49201 cccagcggct gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga
    49261 ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aggcttcacg ttgagcaggg
    49321 gtcgcactct tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat
    49381 ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt
    49441 tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga
    49501 ttggtctggt ctgccgcggt tgccgcactc agaggtgcaa tcaccagcaa agcgaccatc
    49561 attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca
    49621 gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg
    49681 cctttactac cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttagtg
    49741 tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg
    49801 acgtttaatg ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg
    49861 gtatctggaa tgtggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt
    49921 ttcgggtcca gcgtaatatc cgccacccga cccacaacaa cgccaccaat actgaccgga
    49981 gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca
    50041 gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct
    50101 gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc
    50161 aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac
    50221 ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa
    50281 caacgaaatc cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag
    50341 atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc
    50401 tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt
    50461 caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg
    50521 atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc
    50581 tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag
    50641 ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt
    50701 cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat
    50761 aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt
    50821 gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt
    50881 tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg
    50941 cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag
    51001 gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg
    51061 cctgggcact gccatgagcg acaatttttt tgtccgccag gatccaggcg tgatccgcaa
    51121 tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt
    51181 tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag
    51241 gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc
    51301 gcgccatccc accagaaagt tcagaaggca ttagcttagc cgctccacgc agccccacgg
    51361 cctccagctt catcatcact gtactatgca acaatggcgc gggaagttgg gtatgttcgc
    51421 gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa
    51481 ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat
    51541 tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga
    51601 gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca
    51661 cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca
    51721 tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc
    51781 taagcatggc gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg
    51841 gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc
    51901 gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggatgttag
    51961 atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac
    52021 cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc
    52081 ggcatgacgg tggtcagtat tggtacatcg ttaccagaag tcatcgtctc gcttgccgcg
    52141 tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat
    52201 atattgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt
    52261 ctacgccgtg aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat
    52321 gacggacaac ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg
    52381 ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga
    52441 gagcagcttg cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc
    52501 attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg
    52561 gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc
    52621 agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct
    52681 gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg
    52741 ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg
    52801 ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccacaacc gggccgtggt
    52861 gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg
    52921 ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt
    52981 tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct
    53041 tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg
    53101 gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac
    53161 gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga
    53221 tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag
    53281 cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac
    53341 cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc
    53401 gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat
    53461 gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc
    53521 gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat
    53581 gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct
    53641 ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga
    53701 aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca
    53761 gttaagtatt gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc
    53821 cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg
    53881 cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag
    53941 attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct
    54001 gtcagcgccg acgttatggc aaaagcagag aacattcgtc tgctgatcct cgacgttgat
    54061 ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc
    54121 aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc
    54181 attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac
    54241 ttgtatcagg ggcagtcaaa taaactgatc gcctttagcg atctgctgga aaaattggcg
    54301 attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa
    54361 aaagtgggtt taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat
    54421 tacgtgacgc gcattgctgg cggtcgtggc gcagtgcgcg aagtttgcga cttattactc
    54481 ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt
    54541 gggttatcat tgtgctatca ctggcggttc tggtgatgat cggcattaat atggccgaaa
    54601 aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata
    54661 cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg
    54721 ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg
    54781 ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc
    54841 ggatgctcta tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc
    54901 gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag
    54961 acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact
    55021 tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca
    55081 aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca
    55141 tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt
    55201 gatatgcaag gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccatc
    55261 aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg
    55321 attgacggct acggtaaacc ggcaacgttc taccagatgc aggataacgg taaaccggtt
    55381 gaaggtcacg cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt
    55441 aatgcttatc tgcagcaggt tgatagcaac attaagggcg ataagatcac ttacctggta
    55501 aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg
    55561 ccgtcgcagc tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat
    55621 taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc
    55681 gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca
    55741 aacggtgccg gtaagaccac cactttctac atggttgtag gcattgtgcc gcgcgatgcg
    55801 ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc
    55861 cgcggtatcg gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat
    55921 aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc
    55981 gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca
    56041 ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa
    56101 tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa
    56161 cgcatcattg agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg
    56221 cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc
    56281 cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa
    56341 gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga
    56401 acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc
    56461 aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg
    56521 cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg
    56581 aaacgcaaga cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg
    56641 aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca
    56701 ccagcggtga ctacattgac gatgagctgc cggtctatca gggcgaaacg acgcagacct
    56761 tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta
    56821 ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag
    56881 atattctcga aagtatgggc gatgaagaga tcgacatcga cgaggttgaa gccgtcctta
    56941 agcggatcca acggtttgat ccggtcggtg tagcggcaaa agatctgcgt gactgcctgc
    57001 taatccaact ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca
    57061 ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc
    57121 gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc
    57181 ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta
    57241 agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca
    57301 accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc cagtttatcc
    57361 gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc
    57421 tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggtgaag
    57481 aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga
    57541 cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga
    57601 agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga
    57661 ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca
    57721 gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga
    57781 agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca
    57841 accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag
    57901 gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc
    57961 aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg
    58021 catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt
    58081 gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa
    58141 cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca
    58201 tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa
    58261 attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg
    58321 cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg
    58381 gcgaaacaac ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa
    58441 atgggcagta ccggtatcgg caatggtatt gcaattccgc atggcaaact ggaagaagat
    58501 actctgcgtg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc
    58561 gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg
    58621 cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt
    58681 ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact
    58741 ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac
    58801 atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg
    58861 ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg
    58921 gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg
    58981 ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg
    59041 caactactgt tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc
    59101 cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc
    59161 gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc
    59221 gttcacgagc tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg
    59281 acaatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc
    59341 tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt
    59401 cttgataagc ccgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac
    59461 cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac
    59521 ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa
    59581 ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa
    59641 aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg
    59701 cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac
    59761 gtaatgacga aggcaccgag gccgaagcca acagcgttat tgcgctgctg atgttagatt
    59821 ctgccaaagg acggcagatt gaagttgaag cgaccggccc acaggaagag gaagcactgg
    59881 cagccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca tcactttgac
    59941 atacaactcc cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca
    60001 atcaacgtca tttgtacatt ttgtgcgctt ttcactttca gaagaacctt aagaaaacct
    60061 taagaggcat tgtttaggtt ttgtttaagt taatcgacca tactggagat cgtcagaaaa
    60121 tatttccagg agatggcatg attcgcttat ctgaacaaaa tcccctgggc accggaagac
    60181 atcggaaatg ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc
    60241 gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa gtactacgcg catcttggtc
    60301 gccggttaaa agactggagt ggaataccgc gctatcacgg taccgttgag acggattgcg
    60361 gaaccggtta tgtctacgat gtgattgcag attttgacgg caaaccctcc attacgctga
    60421 ccgaatttgc tgagcaatgc cgttacgaag aagacattgc acaactgcgc cagctactga
    60481 aacagctgaa gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga
    60541 atatcctctg tcaccgcatc agtgagtcag aggttatccc ggtcgtctgc gacaacattg
    60601 gtgaaagcac attgattcca ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag
    60661 agcggttatg gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc
    60721 tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa tccagctggt
    60781 gttgttgcat aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac
    60841 ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg aagtggatta ggtaatacag
    60901 ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg aaataacgtt
    60961 gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg
    61021 tcaggatacg ctttttgctc cagactgttt ctatccccag cgttaatcca gcttccagcc
    61081 cttttcgtac ccagctacgc ccatcccata aaaagagatt tttggccgtc tgctgagaaa
    61141 tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa
    61201 tggaagcgac atcaaagccc cagtgctcag gaaatttctg atcttctgcg gcaatcaccg
    61261 ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt gccacgtaac
    61321 gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct gagaagggaa
    61381 caggcgcaac actaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta
    61441 accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc
    61501 cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg
    61561 cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc
    61621 gacaggacag ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc
    61681 agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ctggggcaat
    61741 acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc
    61801 acggtcaacg gtgcattcgc taccaagagt ggcaaaattg cttaaattct tcgccgcgcc
    61861 aacccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg
    61921 gatttcaccg cgcgttattc gtgccgcttc aatcagcacg ttgcgcgttt ccgtcatcgc
    61981 ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa agcagaccgc
    62041 ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc
    62101 gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga tcatcctttc
    62161 gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact
    62221 gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag
    62281 tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt
    62341 ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc
    62401 acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac
    62461 cgacgttatc ttcccaggca ggaaggtccg gagactgaat ttgctgaccc agctgttgca
    62521 gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc tctacaatgc
    62581 cttttttatc ctgcgccgtc agattcgact ccagcacgct gacatagccc ggcatcatct
    62641 tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt
    62701 ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac
    62761 tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaaccg
    62821 aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca
    62881 gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa cgcttcgtca
    62941 gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt
    63001 cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc
    63061 tgttacctaa tttttccagc acagaacgcg caacaatcac gttcagttca atgtcttcca
    63121 ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct
    63181 cttctgctac cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc
    63241 tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa
    63301 taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga
    63361 aaattttatc cagctcatcc tgcggaatgc cgataccaga gtcttccact tcaaaatgca
    63421 gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtaaatttga
    63481 cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct
    63541 gatgcggtaa tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca
    63601 atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa
    63661 gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca
    63721 gcgtgacggc cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg
    63781 tatccagcag aatacggctc aggccgacga taccgttcag cggtgtacgc aattcgtgac
    63841 tgatggtgga gataaacgtc gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc
    63901 gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt
    63961 cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg taatccagcc
    64021 actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt
    64081 ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc
    64141 tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac
    64201 gataaaaaac caggtcgggt gaagcatcaa ggaaggaacg taagaatgag gattgctgct
    64261 cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt
    64321 cctgcagttc tgcttccgtt ttttcacgaa cggcgatttc ctgatttagc tgggcaatat
    64381 tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca
    64441 ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt
    64501 agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt
    64561 cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga
    64621 gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat
    64681 aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc
    64741 aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc
    64801 aacgacctac tccgttggag gtatccacgg acgttccagc gccgatccct gtacgccatg
    64861 ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg
    64921 agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg
    64981 cgtatggcga atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa
    65041 acgccccgct tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag
    65101 ctttatgccg tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg
    65161 cccttcgcca ggcaggccga caattaagtg ggaacacacc ttcagcccgc gctgacgtgc
    65221 cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc gatgcagtgt
    65281 tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct ggtccttata
    65341 ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac caacacacaa
    65401 accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc
    65461 cgcaaaggtg ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa
    65521 attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa
    65581 cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac ggttagggca
    65641 gctaaaaccg ccgtgtagcg tcagcttatg caccttttgc ccataacgac gggtgagatc
    65701 accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa
    65761 agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctggatca atcgtcccgg
    65821 cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca taatcacata
    65881 aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaaa
    65941 cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt
    66001 tcaaggcagg ataagggtta acacgccttt atgacagtca ggaattgact gtttctctaa
    66061 cgacttccct tttagcctta aagataaaat ccattttaat ttcagtaatt taataaagaa
    66121 ttttgcgcta aagcacattt ctgtaccaat aagcttgcca tttgacctgt atcagctttc
    66181 ccgataagtt ggaaatccgc tggaagcttt ctggatgagc agcctgctca tcatatttat
    66241 gcagtaattg agatcccctc ttcaccgtat taaccgatgc gaaaaggaca acaagggggc
    66301 gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca cgctctttct gtgcccgtgc
    66361 gcagcggttc ggaagtgggg ttcccgcaga gcctggggga ggttcacgat atgttgtacg
    66421 ataaatccct tgagagggat aactgtggtt tcggcctgat cgcccacata gaaggcgaac
    66481 ctagccacaa ggtagtgcgt actgcaatac acgcactggc ccgcatgcag caccgtggcg
    66541 cgattctcgc cgatggtaaa accggcgacg gttgcggctt gctgttacaa aaaccggatc
    66601 gcttttttcg catcgttgcg caggagcgcg gctggcgttt agcaaaaaac tacgctgtcg
    66661 ggatgctctt cctgaataaa gatcctgaac tcgccgctgc cgcacgccgc atcgttgaag
    66721 aagagctgca acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc actaacgaag
    66781 gcgtgctggg tgaaatcgcc ctctcctctc tgccccacat tgagcaaatt ttcgttaacg
    66841 ctccggcagg ctggcgtccg cgcgatatgg agcgccgtct gtttatcgcc cgccgccgca
    66901 ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg tagcctgtcg aatctggtga
    66961 acatctataa aggtctgtgt atgccggcgg atctgccgcg cttttatctg gatcttgcgg
    67021 acctgcgtct ggaatcggcc atctgcctgt tccaccagcg cttctccact aacaccgtac
    67081 cgcgctggcc gctggcgcag ccgttccgct acctggcgca taacggtgaa atcaacacca
    67141 tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa attccagacg ccgctgatcc
    67201 ctgatctgca cgacgccgca ccgttcgtca acgaaaccgg ttctgactcc agttcgatgg
    67261 ataacatgct ggaactgctg ctggcaggcg ggatggatat catccgcgcc atgcgtctat
    67321 tagtaccacc cgcctggcag aacaacccgg atatggaccc ggaactgcgt gccttctttg
    67381 actttaactc catgcatatg gagccgtggg atggcccggc gggcatcgtg atgtccgatg
    67441 gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg tccggcacgc tacgtcatta
    67501 ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat ctgggattat cagcctgacg
    67561 aagtggtcga aaaaggccgc gtcgggccag gcgaactgat ggtgatcgac acccgcagtg
    67621 ggcgtattct gcactcggca gaaaccgatg acgatctgaa aagccgccat ccatataaag
    67681 agtggatgga gaaaaacgtc cgccgactgg taccgtttga agatctgccc gatgaagaag
    67741 tgggtagccg cgaactggac gacgacacgc ttgccagcta ccagaaacag tttaactaca
    67801 gcgcggaaga gctggactcc gtaattcgcg tactgggcga aaacggtcag gaagcggtcg
    67861 gttcgatggg cgatgatacc ccattcgccg tgctctccag tcagccgcgc attatttacg
    67921 actacttccg ccagcagttt gcccaggtga ctaacccgcc aatcgacccg ctgcgtgaag
    67981 cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt tgcgaagcag
    68041 agggccaggc gcaccgttta agctttaaat cgccgattct gctctactcc gatttcaaac
    68101 agctcacgac gatgaaagag gagcactacc gcgcagatac gctggatatc acctttgacg
    68161 tcactaaaac cacgctcgaa gcgacagtca aagagctgtg cgacaaagcc gaaaaaatgg
    68221 tacgtagcgg caccgtgctg ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc
    68281 cggttccagc cccgatggcg gttagcgcga tccagacccg tctggtcgat caaagcctgc
    68341 gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac cacttcgccg
    68401 tgttgctggg cttcggcgcg acggctattt atccatacct tgcctatgaa acgctgggcc
    68461 gcctggtaga cacccatgcg attgccaaag attatcgtac cgtgatgctc aactaccgta
    68521 acggcatcaa caaaggcttg tacaaaatca tgtccaaaat gggcatctcc accatcgcct
    68581 cttaccgctg ctcgaaactg tttgaagcgg tcggtctgca cgatgatgta gtgggcctgt
    68641 gcttccaggg ggcggtcagc cgcattggtg gggcgagctt tgaagacttc cagcaggatc
    68701 tgctgaatct gtcgaaacgt gcctggctgg cgcgtaagcc catcagccag ggcggcctgc
    68761 tgaaatacgt ccacggcggc gaataccacg cttacaaccc ggacgtggta cgcacgctgc
    68821 aacaggcggt acaaagcggc gagtacagcg actatcagga atacgcgaag ctggttaatg
    68881 agcgtccggc aaccacgctg cgcgatctgc tggcaattac gccgggtgaa aacgcggtca
    68941 acattgctga tgttgaaccg gcaagcgaac tgtttaaacg ctttgatacc gccgcgatgt
    69001 ctatcggcgc gttaagcccg gaagcccacg aggcgctggc ggaagcgatg aatagcatcg
    69061 gcggtaactc gaactccggt gaaggcggcg aagacccggc gcgctatggc actaacaaag
    69121 tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt tactccggcg tatctggtca
    69181 atgccgacgt cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa ggcggtcagt
    69241 tgccgggcga caaagtcact ccttacatcg ccaaactgcg ctattcggtg cccggagtga
    69301 cgctgatctc cccgccgccg caccacgata tctactctat cgaggactta gcgcagctca
    69361 ttttcgacct caagcaggtt aacccgaaag cgatgatctc cgtgaagctg gtttccgaac
    69421 cgggcgtagg caccatcgcg actggcgtgg caaaagctta tgccgattta atcaccatcg
    69481 caggctatga cggtggcacc ggcgcaagcc cgctgtcgtc agtgaaatac gcaggttgcc
    69541 cgtgggagct ggggcttgtt gaaacccagc aggcgctggt tgccaatggt ctgcgtcata
    69601 agatccgttt gcaggtcgat ggcggcctga aaaccggtgt tgatatcatc aaagcggcga
    69661 ttctcggcgc agaaagcttc ggcttcggca ctggcccgat ggtggcgctc ggctgtaaat
    69721 atctacgtat ttgccatctg aacaactgcg caacgggtgt agcaactcag gatgacaaac
    69781 tgcgtaagaa ccactatcac ggcctgccgt tcaaggttac caattacttt gaatttattg
    69841 cccgtgaaac ccgcgagctg atggcacagc tgggcgtaac gcgtctggtg gatctgattg
    69901 gtcgcaccga cctgctgaaa gaactggacg gtttcaccgc caaacagcaa aaactggcgc
    69961 tgtcgaagct gctggagacc gccgaaccgc atccaggtaa ggcactctac tgcaccgaaa
    70021 acaacccgcc gtttgataac ggcctgctga acgcgcagtt gctgcaacag gcgaaaccgt
    70081 ttgtcgatga gcgccagagc aaaaccttct ggttcgatat tcgcaacacc gaccgttctg
    70141 tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg cgatcagggg ctggcagccg
    70201 atcctatcaa agcgtacttc aacggcaccg caggccagag cttcggcgtg tggaacgcgg
    70261 gcggcgtgga actgtacctg accggtgatg ccaacgacta tgtcggtaaa ggcatggcgg
    70321 gcggcttaat cgccattcgt cctccggttg gttccgcctt ccgcagccat gaagcaagca
    70381 ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg tctgtatgcc gcaggccgcg
    70441 cgggtgaacg tttcggcgtg cggaactccg gtgctatcac cgtggtagaa ggcattggcg
    70501 acaacggctg tgaatatatg acgggcggta ttgtctgtat tctgggtaaa accggcgtta
    70561 acttcggtgc gggcatgacc ggcggcttcg cttacgttct cgatgaaagc ggtgatttcc
    70621 gcaaacgcgt taacccggaa ctggtcgagg tcttaagcgt tgacgatctg gcgatccatg
    70681 aagagcatct gcgcggtctt atcaccgagc atgttcagca tactggctct cagcgcggtg
    70741 aagagattct ggcgaactgg tcaaccttcg ccactaaatt tgcgctggtt aaaccgaagt
    70801 ccagtgatgt aaaagcactg ctgggtcatc gtagtcgtag cgcagcagag ttgcgcgtgc
    70861 aggcgcagta aggggtagca acaatgagtc agaatgttta tcaatttatc gacctgcagc
    70921 gcgttgatcc gccaaagaaa ccgctgaaga tccgcaaaat tgagtttgtt gaaatttacg
    70981 agccgttttc cgaaggccag gccaaagcgc aggctgaccg ctgcctgtcg tgcggcaacc
    71041 catactgcga gtggaaatgt ccggtacaca actacatccc gaactggctg aagctcgcca
    71101 acgaggggcg tatttttgaa gcggcggaac tgtcgcacca gaccaacacc ctgccggaag
    71161 tttgcggacg agtctgcccg caagaccgtc tgtgcgaagg ttcctgcact ctgaacgatg
    71221 agtttggcgc ggtgaccatc ggcaacattg agcgttatat caatgataaa gcgttcgaaa
    71281 tgggctggcg tccggatatg tccggcgtga agcaaaccgg taaaaaagtg gcgattatcg
    71341 gcgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac gcgtaacggc gtaaaagccg
    71401 ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac cttcggtatt ccggccttca
    71461 agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt cactggcatg ggtattgaat
    71521 tcaaactcaa taccgaagtg ggccgcgacg tgcagctgga cgatctgctg agtgattacg
    71581 atgccgtctt ccttggcgtc gggacttatc agtcaatgcg cggcgggctg gaaaacgaag
    71641 acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa cagttaatgg
    71701 gctttggtga aacccgcgac gaaccgttcg tcagcatgga aggcaaacgc gtggtggtcc
    71761 ttggcggtgg cgacactgcg atggactgcg tgcgtacgtc cgtgcgccag ggagcgaagc
    71821 acgttacctg tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc cgcgaagtga
    71881 aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt ccagccactg gggattgaag
    71941 tgaacggtaa cggtaaagtc agcggcgtaa aaatggtacg taccgaaatg ggcgaaccgg
    72001 atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc cgaacatatc gttccggcag
    72061 atgcagtgat catggcgttt ggtttccgtc cacacaacat ggaatggctg gcaaaacaca
    72121 gcgtcgagct ggattcacaa ggccgcatca tcgccccgga aggcagcgac aacgccttcc
    72181 agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc gatctggtgg
    72241 taaccgctat tgccgaaggt cgtaaggcgg cagacggtat tatgaactgg ctggaagttt
    72301 aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc tcctttcttt
    72361 ctggaaagcg cctcgcataa tctgaagaaa accaatgtaa tacgctcatc tggattgtta
    72421 ccggtagatg cccggcgtat agctgattcc attattcaac gcagcgccat ataaagggac
    72481 agataatgga atcgctctca gagagaacct caacaggcta ccagcaaatc cacgacggta
    72541 ttattcatct ggtcgatagc gcccggacgg aaacggtacg tagcgttaac gcgctgatga
    72601 ccgcgacata ctgggaaatt ggccgacgaa ttgtcgaatt tgaacaaggt ggcgaggcca
    72661 gggctgcgta tggtgcgcag ctaatcaagc gactatcaaa ggatttaagt ctaaggtata
    72721 agcgtgggtt ctctgcaaaa aacttacgcc aaatgaggct tttttacctc ttttttcaac
    72781 atgttgaaat tcgccagaca gtgtctggcg aattaacacc attgggaatt ccgcagacac
    72841 catctgcaga attcccatcg gcaaagattt ggcagacgct gtctgccaaa tctttccccc
    72901 tgccctggtc cacctacgtc cgtttacttt ccgttaaaaa cgctgacgcc cgcagctttt
    72961 atgaaaaaga gacgctccgc tgtggctggt ctgttcgcca gctagaacgg caaattgcga
    73021 cccaatttta tgagcgaaca ctactgtcac atgacaaatc agccatgctg caacaacacg
    73081 ctcctaccga gacgcatatt cttccgcaac aggcgatacg cgatcccttt gtgctcgaat
    73141 ttctggaatt gaaagatgaa tattcagaat ctgatttcga ggaggtgctg atcaaccacc
    73201 tgatggattt catgctggaa cttggggatg attttgcctt tgttggtcgg cagcgaaggt
    73261 tacgcattga tgacaactgg tttcgtgtcg atctgctgtt tttccaccgc cgtttacgct
    73321 gcctactaat cgtcgatcta aaagtgggca aattcagcta tagcgatgcc ggacagatga
    73381 atatgtatct caactacgcc aaagagcact ggacgctacc ggatgaaaat ccgcccatcg
    73441 gtctggttct ctgtgcagag aaaggagccg gagaagcaca ttatgctctg gcaggtttgc
    73501 ctaacaccgt tctggcaagc gaatataaga tgcaactacc tgatgagaaa cgactcgcag
    73561 atgaactcgt tcgaacacag gcggtgctag aggaaggcta tagacgccgt taatttcagc
    73621 ggtttcgaat tctgcagaca gtgtctgcag aatctttaaa acgaactaag ttttaagcca
    73681 ttaaatcagc cttaaccttc accacaacct ttttaatctc tccaggctcg ccgacaacgc
    73741 atcctggttt atgcggttca cctggcataa acacggcgaa cattcccggt tttaagatga
    73801 tggcttgctc gttatcaatg gtgctgcaaa gctgataatc atcctcatgg tggaactctt
    73861 cacactggcg cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca
    73921 gttggatatc aatgtattgc tcgtgcaatt ccgctttttt ctcgacgggc gattgagtgt
    73981 taaacgtcat gacattcata aagatattgt cgccctgtaa ttcgtaacga cccggcgctt
    74041 tttcttgcgg tctggcagct aatgccagcg ttaacgcgtc ctgtaacgca ggatgtaacc
    74101 cagcagacgg taatgactgt acttcaccca tcatcataat ttttctccct gggccaacag
    74161 cgcagcccca agtaaacctg catcatggcg gtagtgcgcc gccagtaaat caacatgaaa
    74221 tgccgctggc tcctgcgcca gatacatttc caccaacgcc agataccctt ctgccagacc
    74281 aacgctgcca ccaaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag
    74341 ccttgcaagc gtacgtgcgg agagatgaat cagttgctgc gcctgctcat cgccctgccc
    74401 ggcgcgtgtg aaaatagttc tagcatccgc gccagccagc tcgccctgcg ctgccgccgc
    74461 aatgccgcga ccagaagcaa ttgcttccac gcaacctgtg cgtccacagc cgcagactgg
    74521 gccgtgtgga tcggcaagcg tatgcccgat gtgccccgcc agaccgccag ggccggtaag
    74581 cagtttgccg ccgctcacta caccgccgcc gacgccagtg gaaacggtga taaagaccat
    74641 atcggttata tcgccttcca gcgcctgata ctccgcccat gctgcagcct gcgcgtcgtt
    74701 aatggcaatg gtcggcaaat tggtaagttg ttccagcgtt ttgactaacg gaaagtgtag
    74761 caatccaccg agattatgcg gattcagcgc cagcaaactg ccgtcgcgaa taattccggt
    74821 tgaagcgatg gcaacccgct gcgcatgagc ttgcaacgga gagactaatg cggataaggc
    74881 atcacgcaag gcttctggtg tctggctggc tggcgtagga agttcacgac gatcgcggat
    74941 ctgcccgtca gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccag
    75001 cgtggtcata gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga
    75061 cgcgtaatgg cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atctgccgcc
    75121 tgagcaggcg tgttgtaacg cccttcggca atcacccgac atccggcgtc gctcaacgtt
    75181 ttcaccagcg ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta
    75241 gtgccaataa tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag
    75301 tcggtcatcg ccagtaaacc gtgatggtga atacgtgcca gcagcgtttc aacaggcacc
    75361 ggacgcgggc ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca
    75421 tcgacatctt caatataggc cgtgatgcgt accggagaat cctccagatc gcgtttcaca
    75481 atcccgataa ttggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttca
    75541 atgcgaatgg caaccgcgcc cgcctgctct gccgctaatg ccatggcggc gacgatttcg
    75601 ggtttatcga gcggactgtc cggaaccggc tggcaggaga caatcaggcc accgttagca
    75661 gcgatttttt gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg
    75721 gtacatagac cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc
    75781 caaatggcac ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt
    75841 ccgggcgcag ccaacgctga acgcgggaag gcatatccag accaatcagc aggatcacca
    75901 cgaacgtcag actgaacgag agcgatgcca gcgcagtacc cagatccaga cgttgagcga
    75961 tcaacgcgcc gatgattggg gccagtgcgc cgcccaatgc gccaacgttg taggtaaagc
    76021 ccaggcccgc tgcacgctgg tcggtatcga aataaccgcc aatcagtttc ggtaagatcc
    76081 cggcgatccc ttgtccaagc atttgctgga agaacagtaa cagaccgagc acccagacgt
    76141 ttgcgccgcc aatcgcaaat accggaataa tcagcagctg cgaggccagc aggctacaaa
    76201 cgtacgcttt gcgggttccc agccagtcac cgaggaagcc acctacgcag catcccacgg
    76261 ccgcgccaaa gccactaaag aacagcacat tggcgacagt atgcgggtta taagccagat
    76321 cggttttcag atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcaaaca
    76381 acacgaccac catcagcatt acgcccgttg gccagcgttt acccgtactt tgcaccataa
    76441 agctgataaa gattgcggcg cataacagcc cgagaacagc gacgattgca gcattttgca
    76501 ggttaccggc gaagcagaac cacagcgcag tagccgccgc cagtgtcatt acgatattgg
    76561 caatgcgatg ttcaccacgg tagagaatat ccaccattgt gcgtactggt gctttacctg
    76621 cgtgtttctc tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga
    76681 tgattggcaa aatgccgata aagaacagcg cacgccaacc ccagaccgga accaccaggc
    76741 tatagacctg agcggcaacg acggccccca cagagaagcc tgaaatcaaa aaaccactgg
    76801 ctttgttacg cagatgtttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt
    76861 cacccgccat ccccatgccg atgaccagac gagcgataaa catggtgatg tagcctggcg
    76921 caaagccgca ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac
    76981 gacgcccgta gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa
    77041 aggctgcgga gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt
    77101 cggtgagtac cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg
    77161 cagcggaaaa tgcccgccat tgtgcacgat tgagatggcg ataccacggg atattctggg
    77221 ttgtagtact cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa
    77281 ttgtagggta cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaacaa
    77341 ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt cagttctggc agatattttt
    77401 catctaccgg accaaacggt ttgcggcaca gcggcacaga aacgacatcc atataatgca
    77461 ggacagtttt caggccgcgg aatacgcccg ttttgatcag taaatcaatg actttattac
    77521 attcggtttg cagcttctgc gcggtctgga tatcgccttc tttcagcgcc ttcacaatcc
    77581 cctgataacg ccagcccatg atgttgtagg tactaccgat accaccatca gcgcccgcca
    77641 gaagaccaga ggcgaagatt tcgtcgtaac cgttatagag caccagatca ggatgttcac
    77701 gacggatctg ctccatctga tagagatcgc cagaggtctg tttcagcgca cctacgccag
    77761 gcaacgtaac aagtgtgttg atctgatcca gggtcagttt taccccactc agggctggaa
    77821 tgttgtacac caccatcggc aaaccatccg ccgaatcaat aattgcccga tagtgatcgc
    77881 agtgttcttc aaagctgaaa ggatagtaga acggcgtgac ggcggagacg gcatcgaagc
    77941 cataacgttt agccgatgcc gcaagttgtt ggctttcggc ggtgctgacg caaccgacgt
    78001 gggcgatgag tttaatctta cctttcgcct cttcggcgac gatttccagt acctgttcac
    78061 gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc acccacgtat aaaccgtcga
    78121 tgccctgctg aatattgaac tgaaccaggc gacgcagact cgctttatcc agtgcttgtt
    78181 gttggtcaaa aggagtcagg agtgcagcca ttacgccacg taaattcgtt gccataaata
    78241 cctctgaagt gatgcttgtc tgataaacga tataccttta tacctgttat accagatcaa
    78301 ttaagcaaca ccccatacag aaagcttata atgcgatctg cttcactaaa gtggcattat
    78361 ttctttttgt tggtggtctg accgaaggcg tgccaggtag cagagacgct gttgagatgc
    78421 gattgcaacg cacgatcggc ttcgtcagga tcgtggcggc ggatcgcgtc aacgatcgca
    78481 atatgctgtt gatagctaac gttgttatgc tcgtgcagag cctgatcagc cacggttgga
    78541 cgtgcggcga ttagccagtc gagcagggca acgtggatcg ccatgaagat tgggttacct
    78601 gggatctccg ccagcacgcg gtggaaatca acgtctgaac gaatgaatgc cgcgttgtta
    78661 tccagcgact gactgttgat ttccagtgct tttgccagca aatcgatttg ctcatcggtg
    78721 gcgtgttcag ccgcatagcg caccaggctg gattcaaaga acagacgtaa ttgttcgaaa
    78781 tgggcaatcc caccaggatg agaaaggaaa tctttcgcca tgccggaaag ctcaccgatg
    78841 atagtgtccg cagaaggacg cgagacgcga gcgcgttcgc cgttgtttat ttgcaccaga
    78901 cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag gacgcccgac gttaaagaac
    78961 gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc caaattcacg acggcggatc
    79021 atctgttcca gctcttcttc caccatttcg gagagttttt tacgcgccag cgggcggcta
    79081 cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt gcgaatcaaa tgcgttcata
    79141 aggcccattc tgtaaggtca gtgtgattaa catcatcagt gacatcctat cacaggattg
    79201 aaagtagggg aaaatggcag gtttttctct ttgtgcctca tcattaccat aattaacgga
    79261 ataattaact attgcgaaaa attaatgtaa cgcagataaa aacatcccgt ttgaattatt
    79321 cataagacta ttcacgagca ttatgaatat tatgaatgtg ttcttacaaa ataatcataa
    79381 gcgcatattt tttaatgaaa aaccacctca cctacaatta aaaacacgac atccgcacca
    79441 taaatagact tgcaaaaaat ataacatcat tgttttcaat ctgccgttta tgggattgac
    79501 cgttttcttt tgacacggag ttcaacaatg ttcggcataa ttatatctgt catcgtatta
    79561 attacgatgg gctatttgat cctgaaaaac tacaaacctc aggtggtgct ggctgccgca
    79621 ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg gtggtgtact cgatcccgcc
    79681 aaaagcagcg gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac
    79741 cgcattgccg gattggggct gtcgattatg gcggtgggcg gttatgcccg ctacatggag
    79801 cgcacaggag ccagtcgcgc gatggtgagc ttgctaagcc gcccgttaaa actcattcgc
    79861 tcgccgtata ttattctgtc ggcaacttac gtcatcggcc aaatcatggc gcagtttatt
    79921 accagcgcct ccggcctggg tatgttgctg atggtcacct tatttccgac gctggtgagt
    79981 ctgggagtaa gtcgcctctc tgcggtggcg gttatcgcaa ccacgatgtc cattgagtgg
    80041 gggattctgg aaacgaactc catttttgca gcccaggtcg cgggaatgaa aattgccact
    80101 tacttcttcc actaccagct tccggtcgcc tcttgcgtca ttatctcggt ggcgatctcc
    80161 cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa atatcaatca cgaacaggca
    80221 gagctaaaag ctctcgataa tgtcccgccg ctctattacg ccattttacc tgtgatgccg
    80281 ttaatcttga tgctcggctc gctgttcctc gcccacatcg ggctgatgca gtcagaactg
    80341 catctggtgg tggtgatgtt actgagtttg acggtgacga tgtttgttga gttcttccgc
    80401 aagcataact tgcgcgaaac aatggacgat gtgcaggcgt tttttgacgg catgggtacg
    80461 cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa tatttgcgaa aggcttaacg
    80521 acgattggca ctgttgatgc ggttatcagg ggtgcggagc attctggtct gggcggtatt
    80581 ggcgtgatga ttattatggc gctagtcatt gccatttgtg ccattgtgat gggctctggc
    80641 aatgcgccgt ttatgtcatt tgccagtctt attccgaata tcgcagccgg actacatgta
    80701 ccagcggttg taatgattat gccgatgcat tttgccacga cgctagcgcg cgcggtttcg
    80761 ccgattactg cggtggtggt cgttacgtca ggaattgcag gcgtttcgcc ttttgcggtg
    80821 gtgaagcgaa cagcgatccc catggcagtc ggtttcgtgg tgaatatgat tgccacaatc
    80881 acgctatttt attaagccat taaaagacaa aacaggccac cagggcctgt ttttgtatta
    80941 cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt catcgtcagg
    81001 atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa cggtttcgtt
    81061 gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg cagcttcagg
    81121 ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg cagccagcgg
    81181 cacagaaacc tgacgcggaa tgccgccaaa gcgcgcgtta aagcgtacct catcattcgc
    81241 cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc cgtcacgcgc
    81301 atattccata ggaacctgca cgccagggag cgtcacatcc accaccaagt gcggcgtgag
    81361 ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac gtggtgttag
    81421 ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc tgcttcagtt
    81481 aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt cagctctttc
    81541 gcacccgggc cgctgaactc gatgcccagt tgcggcagac gccacagcag cggagcaaga
    81601 tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc gaagaccggc
    81661 gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc agaagctgaa
    81721 ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg catgtacaga
    81781 cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg gaaacgctca
    81841 tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc caccagggtc
    81901 ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc cttttccacg
    81961 tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg atggctatag
    82021 atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc gacagccatg
    82081 aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg ccagtcagaa
    82141 gttgtgttac ccagtaagga acgactctct ttgttcgaaa atcaaacaaa aaatgagcaa
    82201 tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga cctttgtggt
    82261 gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag ttgacttagt
    82321 cccttattgg cgatgtggtt tttgttttgc ctgtctgtca gacggtagca aaaagcaact
    82381 ttccaatttt tacgctgatt cagaatttaa ccataaaaaa acccgccgaa gcgggttttt
    82441 tcgcaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact gcggacgacg
    82501 acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac gagtaacgaa
    82561 gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag cgcgggtgat
    82621 accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg tgatgtacag
    82681 gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca tgcgagcagt
    82741 ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac cgttgcccgg
    82801 tttgatgaaa acgcgagctg cggaactttt gcggcgacca gtgccgtagt attgattttc
    82861 agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg ccgcgtggtt
    82921 gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca gcgggccttt
    82981 tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag caatcatctc
    83041 ttcaaaggtc gcttgtttga taccaccgat gtggccggtg tggtgatagt acactttgtc
    83101 agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga tgtaatcacc
    83161 ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgcaggcgac gagccagttc
    83221 agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt cgcgttttac
    83281 ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat agttacacgt
    83341 tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc ggcaaaccta
    83401 ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact ttcttgtaac
    83461 gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt tgtgatttgt
    83521 gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg catctcttgc
    83581 agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata aatttcatac
    83641 agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc atccaccagc
    83701 gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac atcaaacaac
    83761 atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg ggcgtcgacg
    83821 cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt cgccaatggc
    83881 cgatggttga tttctaacgt cggtgaattt tctcgtttcg cccccgccag cgccaaccat
    83941 agtttatcca tctgcgcccg ggtttcatcg ttgagtggcg aaagccacag atgcgcctga
    84001 gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc acaatgctgt
    84061 ttaatggcat cgattgcagg cagaaaacgc gcacgttgca ggccatttcg ataaagttca
    84121 tccggcggaa tatttgacgt cgctaccagg gtaatgccgc gggcgaacag ggctttcatc
    84181 agaccgccaa gtagcatggc gtcggtaata tcagaaacaa aaaattcgtc aaaacagagc
    84241 acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt ctgcccctgt
    84301 aaggcagtta gctcttcgtg cacccgcagc ataaaacggt gaaagtgcag gcgctgtttc
    84361 cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc gcgtcctaca
    84421 ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg tttaccccac
    84481 agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcct gctattgatg
    84541 agttcttgat aaataatttc caggcggctg acggcctctt tttgaacgtc gtcgggttga
    84601 tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct ttgcatgatc
    84661 ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa aaaaaggccg
    84721 ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg gttatagtgg
    84781 cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa aagacaacgg
    84841 gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat cattattggt
    84901 gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt gcagtacgaa
    84961 ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag ccactttgcc
    85021 cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta tcagcacatg
    85081 gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc gttccgtaat
    85141 cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc tcgcgactat
    85201 tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt tatttttcgg
    85261 gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat ctggtgtctc
    85321 attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt ttcttatcat
    85381 tcaggtacga gagcaggaat aatgaaaaaa caaacccagc tgttgagtgc attagcgtta
    85441 agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc aggccaggtt
    85501 gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct tccggcagtg
    85561 gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga agaattcaaa
    85621 aagttttttg gtgatgattt accggatcaa cctgcacaac ccttcgaagg tttaggttcc
    85681 ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt gattaatcag
    85741 gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa actgattggt
    85801 agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa attaacgcaa
    85861 atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc ggtcggtaac
    85921 ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg ccgcagcggg
    85981 ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa ccgcggtaac
    86041 tccggcggtg cactattaaa ccttaacggt gagttaattg gcatcaacac tgcaatcctt
    86101 gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat ggcgcgaaca
    86161 ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg catcaaaggc
    86221 accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg tggcgcgttt
    86281 gtcagcgaag tgttgccagg ttctggctcg gcaaaagcgg gcgtcaaagc gggcgatatt
    86341 attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc tcgtatcgcg
    86401 actacagagc cgggcacgaa agtgaagctt ggcctgctgc gtaacggcaa accactggaa
    86461 gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat gatcacgcca
    86521 gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa aggtattaaa
    86581 atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa agacgatgtg
    86641 atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa agtgctggcg
    86701 gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat ctatctgctg
    86761 atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt aactcgtggt
    86821 atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag ctcttacgtt
    86881 ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct tcgctgcgca
    86941 gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct gccagttata
    87001 atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt ggtttgaaca
    87061 ccaactctca caaccagctt gagatccgca ccttgggatc cggtgtaatc atggatcaac
    87121 gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc atcgtcgcct
    87181 tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta accgatctgg
    87241 cggtacttaa aattaatgcc actggcggtt tacctaccat tccgatcaat gcacgtcgcg
    87301 taccgcacat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc gggcagacca
    87361 ttacccaggg gattattagt gccacgggtc gaatcggtct gaacccgacc gggcggcaaa
    87421 acttcctaca aaccgatgct tccattaacc acggtaactc tggcggcgcg ctggtgaact
    87481 cgctgggaga actgatgggc attaacacgc tgtcgtttga taagagtaac gatggcgaaa
    87541 cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt atggataagc
    87601 tgatccgcga tggtcgcgtg atccgcggct acattggtat cggcggacgc gagatcgcac
    87661 cactgcacgc gcagagcggt ggtatagatc aactgcaagg gatcgtggtt aatgaagtgt
    87721 cacctgacgg cccagcggcg aatgcgggta ttcaggtcaa cgatctgatt atttcggtgg
    87781 ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa attcgccctg
    87841 gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg caggtcacca
    87901 ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaccg gagtctgtgc
    87961 tccggttttt tattattcgc taattaatta cttattaacg aactcttcgc ccagggcgat
    88021 atctttcttc agcgtatcca gcataccttc cagtgcgctc tgttcaaatg cgctcagggt
    88081 accgatagat ttacgctctt ccacgccgtt tttacccagc agcagcggtt gagagaagaa
    88141 acgggcgtac tgaccgtcgc cttcaacgta ggcacattcg acaacgcctt gttcgccctg
    88201 cagtgcacga accagagaca gaccaaaacg tgcagctgcc tggcccatag acagggttgc
    88261 agacccacca ccggctttcg cttcaaccac ttcagtacct gcgttctgga tacgtttggt
    88321 cagatcagcc acttcctgct cggtaaaact aacgccagga acctgtgaca gcagcggcag
    88381 aatggtaaca ccagagtgac cgccaataac cggcacttca acttcgcctg gctgtttgcc
    88441 tttcagttcc gcaacaaaag tgttggaacg aatgatatcc agcgtggtaa cgccgaacag
    88501 tttgtttttg tcataaacac cggctttttt cagcacttca gcagcaatcg caactgtggt
    88561 gttaaccggg ttagtgataa taccaatgca cgctttcggg caggttttcg caacttgctg
    88621 taccaggttt ttcacgatgc cggcgttaac gttaaacagg tcggaacgat ccatacccgg
    88681 tttacgcgct acacctgcag agataagaac gacatctgcg ccttccagcg ccggagtcgc
    88741 atcttcacca gaaaaacctt tgattttcac agcagtaggg atatggctca gatcgacagc
    88801 cacaccggga gtcactggag cgatatcata cagagagagt tctgaacctg aaggcagttg
    88861 ggtttttaac agtagtgcaa gcgcctggcc aataccgcca gcagcgccga ggactgcgac
    88921 tttcatccta aactccttat tatattgata aactaagata tgttgctccg ctgccgcgac
    88981 cttaatccac aaaattgccg tttacaatga ccacatctca agaatgtgta gtcacgcaag
    89041 tttagcgttt atgcatttaa ttgccgtaat caggaaccta acttacgtaa ttaacagtcg
    89101 cttatgcatt agcgcaacat tccaacaggt ggtgacaata taccctaccg ttcagccaaa
    89161 acaacatcaa tttgataaca attaatttac ttttaagcag aatttgcatg ccgtgacgca
    89221 ggcatgtttc tcaataacga aatttgataa aatcccgctc tttcataaca ttatttcagc
    89281 cttcttcagg gctgactgtt tgcataaaaa ttcatctgta tgcacaataa tgttgtatca
    89341 accaccatat cgggtgactt atgcgaagct cggctaagca agaagaacta gttaaagcat
    89401 ttaaagcatt acttaaagaa gagaaattta gctcccaggg cgaaatcgtc gccgcgttgc
    89461 aggagcaagg ctttgacaat attaatcagt ctaaagtctc gcggatgttg accaagtttg
    89521 gtgctgtacg tacacgcaat gccaaaatgg aaatggttta ctgcctgcca gctgaactgg
    89581 gtgtaccaac cacctccagt ccattgaaaa atctggtgct ggatatcgac tacaacgatg
    89641 cagttgtcgt gattcatacc agccctggcg cggcgcagtt aattgctcgc ctgctggact
    89701 cactgggcaa agcagaaggt attctgggca ccatcgctgg cgatgacacc atctttacca
    89761 cccctgctaa cggtttcacc gtcaaagacc tgtacgaagc gattttagag ctgttcgacc
    89821 aggagcttta atctctgccc cgtcgtttct gacggcgggg aaaatgttgc ttatccctct
    89881 caaccccctg ctttcccctg cgattaattt aacgaatagt gcgttttact gcgacatgtc
    89941 attcacacaa tgaatacata aggtaaaaaa agcacattat gcaaaattca ttatctaatt
    90001 gaaaacacta gaattaacga taaataaccg tatttttaat tcttttttgt tattaacatt
    90061 cacattttta acacttagta tcaacagaaa cagttagcgt ggtattaatt agttcaataa
    90121 ttagtgtata cttgattttg tgataagggt cacgaaacaa agacccagca aaaagattat
    90181 gtcgaggtaa aaatcatgaa aatcaaaacc actgttgctg cattaagcgt actttctgtt
    90241 ctctctttcg gtgcattcgc tgccgactcc attgatgctg cacaagcaca aaaccgtgaa
    90301 gcaatcggga ccgtatccgt aagtggtgtg gcgtcttcgc caatggatat acgtgaaatg
    90361 ctgaacaaaa aagcggaaga gaaaggcgca acggcctacc agattactga agctcgtagc
    90421 ggtgacacct ggcacgctac ggctgaactg tacaaataaa ccctcatcgt cttgtccgac
    90481 gatattgccc ccggttcggg ggcttttttt tgcgctaatg acgaacatta aaacgcaaat
    90541 gcccttccag ctcttcctct gcctcatcaa acagcaatat taacgcgcca aaacggcgac
    90601 gcgttttctc tcccagatga acaaattcaa tctcaagtgg tagcggcagg acatcgttca
    90661 ttaacacatc ccatagtgag tcgagatcgc gtaccttatc tttcgccaga ccaaaggctt
    90721 ggctgaagtc gcgataaaaa tcctcctgac tctcaatctc atcaaaatca aaggtataaa
    90781 tattcatctg ttgccaccgt cacgtttcgc ggcgagttct gccgttcatc gctaagtata
    90841 gccatgaaaa aaccgacgct tttggcgtcg gttttgactt aactatcggt caacgcatgt
    90901 tgatacttat ggagcatccc cgccagccga tgaaccggtt ccgtcacctg cggtggcgct
    90961 tgccagatgc gtagcttttc ctggtagatt tccagttttt ccagcaactg accaaagtac
    91021 cgacgacgtt tatcatcgct acgggcagat atcacatggt ctgctgtgcg gcgcatttgt
    91081 cggtgaaacg ccgataaatc ctcgttaacc gggatcggcg catcacgcag gcgctggtgc
    91141 gcgataatca tcgtcagcgc caggcgaaat ttcggcaaat cccctgggaa cttattcatc
    91201 agcaaaaaca gctgctgata aagtgccggg aggtggttct ctttacgacg tgccacattg
    91261 gtagtcatcg cggaaacagc ggcagaaaca aactgattaa gcagtacacg tccagtcctg
    91321 tcgcgcgatt tatcccgcac cagcaaaata acggtgaacg cgagcacaca gccgacgatt
    91381 tgccctaatg cgctgtcgag aaactgactg aaatggaaag tcatcgggtt atccagcacg
    91441 ataatattta tggtgctggc cagcgccccc atcgagccca gtcgccgttt ctgtacttct
    91501 attccgagga agaatcccag cactgccagg ctaatacaca gcagcaacat gctctgttgg
    91561 gtattaggga taatcaccaa aaagtagagc agccctaacg gcagcgcggc cagcgtcccg
    91621 tagataaagt cgatcgccac catgcgtgga ttcggcaaac gcattgccag tgacgtcacg
    91681 accgcaatca tcaccattgc accactgccg gaagtccagc ccgtccacag ccagaaaagc
    91741 gttcccagaa tgcaggaaag tgtggttcgc cagaagttaa ccatcgcatg atgacgttcg
    91801 gctgactcta ctttgacttc cggttcgcct tgcaggatct cttcttcggt ggcgttgatt
    91861 tttgtgttac tgataacgcc gcgcttgaga agctgataac gcgttgccgc cgcgacccag
    91921 ctataaatgg tgacaggcgt ttcccgttcc ccggtccagg cgataactct tcgcaggcgt
    91981 ttgagctgct tgtggacgtc ctgcgcggtt tctaccggcg tgtcaaaaaa ttcgcggaaa
    92041 gtatcagtga tcaattccgg gcgcgtattc tgaataagat aagtttcgca ggattgggta
    92101 atcagcgtca gcgatagcgt attgatcgct tttaaacgtc gattggcccg cgcccagcgg
    92161 gaagattcca tattcaggtt gctgcgcata ccttgtagcg ccgtggtgcg tcgcaccagg
    92221 tcgccccagg ctttatcgac aacttcacca tcgccatgct tgatacagag ttgcattaat
    92281 tgatattgcg cgaccagcaa actttccagc tctcgatcca cttcttgttt gatcgatcgc
    92341 ggagaaaaga gcaaatccgc cataatcgca cacacaatac cgataacgat ctcgctacaa
    92401 cgttcgacgg caaactgcgg cgtaagcaat ggttccggct gaatggtgat cacaatgatc
    92461 agcgcggtat aaccggccag cccccacgcg tacgagtttt ctattcgtac cagcgaggat
    92521 atccaggtac aaaaaccggc ccagatacag cacaccagaa tcatcaataa tggtgcgcgg
    92581 atcatcgcaa tgatgatcac cagtccggca atacagccaa taaatgtgcc gatgatgcgc
    92641 aaaaagccac gatagcgaat agcgccagaa tacggttcac ctcccgcagc aaaggccgga
    92701 ccggcggcaa caatcgccgc tgtcagtacc gcccagcgtg gcgtttccag ctggaagtga
    92761 aagccaacaa acagcgccag tacaatggcg gtcgccagtt ttaccgcaaa gcgaatatgt
    92821 tggttagcaa tggagaaaat acccatcgtg attaaccaaa ctcacgcagg cgatgggcca
    92881 ttttacggaa gaacgaatcc tggctttcgt cgcgatcttg tttgccagtg accaccactg
    92941 tagcagtggt gcccgcaggc cagatgttct cttgctggtt gtcgagacga atacgaaccg
    93001 gaacacgttg cgccaaacgc acccattcaa ggttagagtc gatggtcgcc atccctttgt
    93061 cgtcacgcgt gctgctggcg ttggtgaccc cagcggcaac actatcaaca gtccctttca
    93121 gcactttgtt actgccaagc ggcgtgatct ctgcacgata ccccggacgc accccttcca
    93181 gcttagtttc ttccatatag gccagtacat agaaggagtt ctgtttcacc agcgcaaccg
    93241 ccgttgatcc tcgagtaata aactcaccgg tatagacgtt gaggttggtc acccagccat
    93301 ctgctggcgc gcggatcacc gtgcgttcaa gatccagttt tgccagatcg cgggtcgcct
    93361 gcgctttcgc taactgatgc agaaccgttt gtagtacgtt gttagcctgg tcgatctctt
    93421 cgcgagacat cgcctgcaca ccgagacggt tacgacgtcc ggcctcctgg cgtttctcct
    93481 gtgccagtac ctgataataa gcaacatcgg cttgcgcttc ctcaagcgcc ttttgatagc
    93541 gcggctggtc gatggtgaac agtatctgtc cttttttcac cagctggtta tcatgaacat
    93601 tcacctgggt aatgagtcca gaaacgtccg gcgcgatcgc aacgacgtca gcgctaaagc
    93661 gcgcgtcacg cgtccagggg gattcggtgt aatagaccca ggcattaaaa attgcgatga
    93721 aggccagaat gactaatacg accgtgatgg ccgtacggga gaattttctt attagtgttt
    93781 tcacttcaac ctcaaacgaa cagtcgcgat atcaaataaa acaagcagca atagagcgcg
    93841 gtgttgaaca acgccggatg ccagacaaag tcgtagatac ctgttggcac aagtacccgg
    93901 cgcaccagcc agaaaatcgc cagtgataaa agcaattcaa aaaatatcgg tgggaaggac
    93961 agcccaaaca ccacgataac gggaaacaga ctcatgttga ccttggttgt atagagagag
    94021 caggcgttat tattttcagc atctgtcgcc gcagagaagg gcatggaaag ccgggcgaga
    94081 gcaacattgc tgtagattga tatttaatat attagcgtaa ctgttatgct gttatctata
    94141 ttatgtgatc taaatcactt ttaagtcaga gtgaataatg gaacgactaa aacgcatgtc
    94201 ggtgtttgcc aaagtagttg aatttggctc ttttaccgcc gccgccagac agctacagat
    94261 gagcgtttcg tccatcagtc agacggtgtc aaaactggaa gatgagttgc aggtaaagct
    94321 gttaaaccgt agcacacgca gcattggcct gaccgaagcc ggtagaattt actaccaggg
    94381 ctgccgtcgt atgcttcatg aagtgcagga tgttcatgag caactgtatg ccttcaataa
    94441 cacccccatc gggacgctac gcattggctg ttcttcaact atggcacaaa atgttctcgc
    94501 cgggctgaca gccaaaatgc tgaaagaata cccaggtttg agcgtcaatc tggttaccgg
    94561 aattccagcc cccgacctga ttgccgacgg tctggatgtg gtgatccgcg tcggcgcgtt
    94621 gcaggattcc agcctgtttt cccgccgtct tggcgcgatg ccaatggtgg tgtgcgccgc
    94681 gaaaagctat ctcacacaat acggcatacc ggaaaaaccc gccgatttaa gtagccattc
    94741 atggcttgaa tacagcgtgc ggcccgacaa tgaatttgaa ctgattgcac cggaagggat
    94801 ctcgactcgc ctgatcccgc aagggagatt tgtgactaat gatccgatga cgctggtgcg
    94861 ctggctaacg gcgggtgccg ggatcgccta cgtgccgctg atgtgggtga tcaacgagat
    94921 caatcgcggt gagctggaga tcctgctgcc gcgttatcag tcagatccac gcccggttta
    94981 tgcgttatat accgaaaaag ataagctgcc gctgaaggta caggtcgtga tcaactcgct
    95041 gacggattat tttgttgagg tcggtaaatt gtttcaggag atgcacgggc gcgggaaaga
    95101 gaagtaattc atattgtact gttacgttct acaaacctgt gccaacgggt tcccctcacc
    95161 ctaaccccag aggagcgagg ggaccgttcg tgcacgtaga aagattaatt atccttctga
    95221 aaataatgaa attattacgc agtaccgcca acagtcaggt tatcaacttt cagcgttggc
    95281 tggcccacgc caaccggcaa actttgccct tctttaccgc agacacccac gccgttatcc
    95341 agtttcaggt cgttgccaac catcgaaatc tgctgcatgg tttcgatacc ggaaccaatc
    95401 aacgtcgcgc ctttcaccgg cttcgttact ttaccgtttt caatcagata cgcttctgaa
    95461 gtggagaaaa cgaatttgcc ggatgtaata tccacctgac cgccaccaaa gttcggtgca
    95521 tagataccgt actcaacgga ttcaataatt tcctgcgggg tcgatttacc cggcagcata
    95581 taggtgttgg tcatacgcgg catgggcaga tgggcgtagg attcacggcg accgttgcca
    95641 gtcggcgtca tccccatcaa acgcgcgttg agtttatcct gcatgtagcc tttcagaatg
    95701 ccgttctcaa tcagcacgtt gtactggcct ggcgtacctt cgtcatcaat cgccaccgaa
    95761 ccacggcgat cgaccatcgt gccgtcatca accacggtac acagttctga agccaccagc
    95821 tccccgacct gcccactaaa tactgaagtg ccgcggcggt tgaagtcgcc ttccagaccg
    95881 tgacctaccg cttcatgcaa cagcacgccc ggccaacctg cgccaagtac taccggcatg
    95941 gtgcccgctg gtgcagcaac ggcagaaaga ttgaccagcg ccatacgcac tgcttctttt
    96001 gcccatgcat ccgcacggac ttcgccgtca agatcggcaa ggaagaactc ataaccaaaa
    96061 cgaccgccgc cgccactggc accgcgttcg cgtttgccat cttcttcgac cagaacgctc
    96121 acggaaagac gcaccagcgg acgaacatcc gccgccagcg tgccgtcggt ggccgcaacc
    96181 agaattaatt cataaacgcc gctgaggctg gcagtcactt cttgtacgcg cttgtccgct
    96241 tcgcgggcaa ccttatcgac gcgacgcagg atatccagct tctcttcacg gctcatgctt
    96301 tgcagcggat ctaccgaggt atacaacggg ctatgctcta ccgcgcccag cgtctgtact
    96361 ttgccatcac cactatcacg gacgatggtg cgcgccgctt gcgcactctg ttccagcgcc
    96421 agcaggctga tttggtcagc gtaagcaaat ccggtttttt caccactgat tgcacgcaca
    96481 ccaacgccct gatcgatgtt gtaagaacca tctttaataa tgcggtcttc taaaacccag
    96541 gattcgtgat agctcgactg aaaatagaga tcgccataat caaggcgacg ttcggccagt
    96601 tgaccgagga tcgcgaacaa gtcctgatgt ttcaggccgt tcgccgctag caattgttca
    96661 cttaccaggt taagactcat cgtttttgct actcgttagt tactgcagta gaggattttt
    96721 tacggctgcc ggatgcggcg tgaacgccct atccgaccta cggttatgtt ctggctcgtt
    96781 ggcctttggc aacgattatc atatgagatt ggggcaatta cgcgccctcg tcaaatcatt
    96841 gcgctttttc tttacgcggt tggcgcaaca cttcgttgat ttgcggatcg tccagcggac
    96901 ccgaaatgtg atagcgcaaa atggagactt tgctccacag cggccccagc actttactgg
    96961 ctgcaaatac tgccgcgcca acaatggggt taaccgcaaa agccgcagcc acgcccaccg
    97021 tcgcagaaat ctctggtgcg acaaccgctt ccatattcag gtcgcgacgt accagattta
    97081 ccgacccttt catggcgata tccgcctcca ggccatccac cagcgtgtcg tcggtgtgca
    97141 taaccccgtc tttaatccac gcggtgctgc gaatggagtc aaaatagaac ccttcgccaa
    97201 aagtgtctct gaaatcaaaa cgcagcttac gcatcagggc atctacgctc aataagcgca
    97261 gcaattgccc ggcatgtccg gtattgattt cggtaatttc gcctttaccc agttgagtat
    97321 gaatgatgcc attcaacgtc gcttcatctg gctgccaggg tgctttgcgc cagtgtaaat
    97381 cgtaatccac attaaatgac gactggcgta tgggcgtcgt gacaccaaaa aattctgcgg
    97441 cggcatcaat tttctgcccg cgcagttttc ctttcagcga ggtacgttca ttccccggat
    97501 tattaaccca ttcaccatcg gcagtaagcc gcgagaaacc agtatcaatc agtccattgg
    97561 tcagcgttaa cgtatcgcca gaaatggtga gatcactgtc aatacgaccg aatttttgcc
    97621 cccagaacca gcactctgtg catcgtattt gtgcgtccgg ccagccacgg aagttaatgc
    97681 gctccgttgt cgggaaaggt gatgacggtg ttgaatcacc acgagttttc gccacgctcg
    97741 ggttgtaata aagatatttg atattcgcca gccacggcgc gttattacgc atcgccagcg
    97801 tggcattgat ttcacgcccc tgcgcctcaa ccagggtgcc atttgccgtc ggttgcgaaa
    97861 caatactcag gttattccat tgctgatttc ccagtgacaa cataggcgta cgtaacgtta
    97921 tgtgttgtgg gaaactcgct gcaccaccga cactctccgc agcgcctttc tggaacaggg
    97981 ccagccactc ggcaccattc atcggcggca tattgagttc aacgccactt tgttccggca
    98041 acggcgggag cgttttactg tctgccgccc aaatagcacg atcgagcgtc agcttttgac
    98101 caagcaacca gcggctattg aaatggttat ccgcaccagc ctgtccggtt aattcaaagc
    98161 tgttgagatt gccatcaacc ttaacgttta ccgctagtgg ttcacccgca ggtttggcta
    98221 acggtgaagg taagtgactg ctcacattct tcagatcgcc gttcagctct acgttatagg
    98281 tcgcgccagc atgataaggc agctcaatgc ccactttacc atcccacgcc acgctgccac
    98341 tcaatgcttc gttcaccgct tcaggcagaa cgccggtttt cgccggttgc cagttaccgt
    98401 tgaggtttac cgcaacctgg taggcttttg ccccttcttt ggtggaaaaa tccacgttca
    98461 acggctgatt aaaccagctt gctgtcagtg gttcactttg cagatcgcca ttgataaagc
    98521 tgaatttacc gctcaaattt ttcagggtgc tgtcgagtgg tttgataaac agactgttat
    98581 tacgcagcgt cacttcacct ttcgcggtta ccagctcgcc gttcagcggg atatcaagat
    98641 gtaagcgagc attcacatcg ccgtcgagct ggagttcttg cagggtcgca cccagggaat
    98701 ctttcagcgg tgtctcatca aagtaagggc ctacggcttt acccggacct ttaatgtcag
    98761 cgtcaatcag cagtttttct tttgagtagt cagggatcac tgcggtgaga ttactcgcgc
    98821 gcacgccgcc cagattaacg ccatcggttt tcatccataa accgtcgtta ataaagtcca
    98881 gttcaatatc aaggttagtt aatgcaggcc agtccggctg gaaggcaaac ttcgcgttgc
    98941 gcagcggcac cagcacttca aactgacctt cgttgtgttt ataggggaag agttgcggat
    99001 tgccaccata aaccagcgtc gcgttatccg cttcaccgcc ctgaatcgcg ccacttaagt
    99061 aatcaaccag gtctttaccc atcaagtttt ccgggaaata gcgccaggct tgtgaaccat
    99121 catcggtact gatgccagcc agaataccca gccagggttc atcgttagca ggttgcaggt
    99181 aacgaaaacc gccgcgcgca tggacggctt tggctttaac gtcaatatta cgcccatcca
    99241 gctggaaacc tttgtcattg ttcagccagc ttatagttgc ctggccgtcg gcgatttcca
    99301 gtggcgcacg gaataccgtt tcgtaaggca tctttgcctg cttcatcgac gcggtaagca
    99361 aaccattttc aacgctgccg gaaagcgtcc cggagaagtg ttccgcaccc ggtaataatt
    99421 tccattgctt ccaggccaga tcgctccacg atgcctgaaa acgggtattg tctgccgcct
    99481 gaagcgggat atccagcgcc agagtgttaa tcttgccgct cggttgtgtg gagcgccaaa
    99541 catcacccag tgcaggtgaa agtttcgcgg ccagcgggcg tatgccctcc aggcctgcca
    99601 gctccagatt actggcgcga atccggagtt cgtcactgcg tttattgtct ttgccgccaa
    99661 cgtcctgttc cggtatccag gccagcgtca atgctccgct cggccagggt ttaccgtcca
    99721 tcgtgatccg tgtatcggga atagagaact gccagcccgg attttcacgc gtaatatgcg
    99781 cggtcagatt atccaccgac agcgtatgcg tttccttctc gcccaaccag ctggcaccgc
    99841 cctgtttcag ccagacgtca ccgccggtta catcgccttt gtcgatcgtc atccagcctt
    99901 caagggagaa ctgtgccgtt tccagcgcaa tattgtcctg catccattta ccgagccacg
    99961 gcttcaggtc gatgtcatcc gcctggagcc agacgcgacc attgcttaac aacccctcat
   100021 catcgcgcaa atccatgcgc acctgcatca cgccatgctg tccggtaagg ctggagaggc
   100081 ttaccagacc ttccgcacgg tgtcgacgtg gatcgttcag ccaggtgagt tgtgggatcg
   100141 ccagctcggc gcgctgaccg gatggcgtca ggaaactgac ttcactgtcg cggagatcga
   100201 aatggtcaaa ttgacgaaga aacagatcgc tgatgtgact ggcttccaga ctgtcatcgc
   100261 taccaccgct ggtgatagga gtgttggtgc gaaagcgcag ctgccagaaa gtgaggtcgc
   100321 gaaactgcca gcgcatatgt aacaggctct gccagacatc cagcgccaga gtaacgcgtt
   100381 taaccgaaaa ttcgccgcca tcttttagtt ctgcacggat gtcgtgtgct tcaagcgtcg
   100441 ggccaaaatt ctgccagctg gctgagagct gactggcttc taccggcatg ccagtcgcgg
   100501 attctatttt gttgaggatt tccggacgcc aggcgtcaag atgcggtaaa gcaatacgca
   100561 ggccgctaac cagcagagca gcgatcacaa cgagcgcggc tccagtaagc agtaaaatcc
   100621 ccggcaatcg cctcacccgt cactccttgt ctgctaaaaa tgtgactcaa aaaccctttg
   100681 ccggatggcg gcccagcatc tgtttacatc attacgacgt caaactgctc ctggttatag
   100741 agcggttcaa tttgtacttt aacctgtttg ccaacgaaaa tttccacttc cgccagcgag
   100801 tgtgactctt cgcctttcaa ggcttcagct actgccggag atgcatagac caggaaacgg
   100861 tcggagtcgt aagcatggtg gacacgaaca atctcgcgca tgatttcata gcataccgtt
   100921 tccacggttt tcaccgttcc gcgaccgtgg caggttgggc attcgttaca cagtacgtgc
   100981 tcaatgcttt cgcgggtgcg tttacgcgtc atctccacca gccccagcgc cgaaaaacca
   101041 ttaacgctgg ttttcacccg gtctttgctc aacgcctgct ccagcgagtg cagcacgcgg
   101101 cggcggtgat cttcattatt catatcgatg aaatcaataa tgataatccc acccagatta
   101161 cgcaaccgca actggcgagc gatagcctgc gtcgcttcaa tattggtatt gaaaatggtg
   101221 tcgtccagat tgcgatgacc gacaaacgct ccggtattga tgtccacggt ggtcatcgct
   101281 tcggtctggt cgataatcag ataaccaccg gatttcagtt ctactttgcg ttccagcgct
   101341 cgctggattt cgttttcgac atcaaagaga tcgaaaatcg gctggcgtcc ggtgtaatgc
   101401 tccagcttgc tggtcatctc gggaatgtac tccgaggtaa actccagcaa tgcttcgtaa
   101461 gtcaggcgtg agtcaacgcg aatgcggtcc agttcggcgt cggcgaaatc acgcagaaca
   101521 cgctgcgcca gcgccagttc gccgtacagc tgataacggg tctgcgggcg ttttttacgc
   101581 tccataactt tggtccagac gcgtttcaga taagcggcat cggaggccag ttcagcctcg
   101641 ccaacccctt ccgctgcggt acggatgata aacccgccct gctcgtcgca atactctgcg
   101701 accacttttt tcaggcgttc acgttctgat tcgctttcaa tacgttggga aaccccaacg
   101761 tgagaagccc ctggcataaa caccagatag cgagaaggca gcgtgatatc ggtggtcagg
   101821 cgcgcacctt tagtgccaag cggatctttc accacctgca ccatcagatc ttgcccctga
   101881 cgaaccagtt ccgagatgtc gcgcaccgtg aattgctttt gttcttcacc cgccacacat
   101941 tcggtgtgcg gcatgatatc ggatgcatga agaaacgcgg ctttatccag cccaatatct
   102001 acaaaagccg cctgcatacc cggaagtaca cgacttacac gacccttgta gatattgcct
   102061 actattccgc gtcgcgcctc acgttcaata tgaatttcct gcagaatacc gccatcaata
   102121 tacgccactc gcgtttccga aggcgttacg tttactaaca attcagccgt catgtttatc
   102181 ccttttctca cgcagtgcgt taaaattact taataattca tacgtttcaa ccagcggtaa
   102241 gccgactacg gcgtgatagc tgccatttat cttcctgaca aaacagccac ccagcccctg
   102301 aataccgtat gcacctgctt tatctaacgg ttcaccgctg gcgacatagc ccgcgatgtc
   102361 ttcgtctgtt aacgttctga aagtcacatc ggtgaccacc aggcaatcga gaatgtgctg
   102421 gctgtcggcc aacgccactg ccgtcatcac ctgatgggtc tgacccgata atttgcgcaa
   102481 catctgcgcc gcatgctctg cgtcgcgcgg tttctccagc acttctccat tcaggataac
   102541 gatagtatcc gcacccagca ccgggagatc ctgcgccgtt tgcgcgacac ccgcctgtgc
   102601 tttctcgcgc gccagacgca caacatactg ctgcgcgctc tcctgcggct gacgctgctc
   102661 ctcaatgcct gtaacaatac gttcaaaggt cacgccaagt tgcgcaagta actcctgacg
   102721 acgcggagaa ccggaagcta aatacagaga agtcatagaa acctttattg cactgcaaac
   102781 tgctgacgga ctttacgcat cagcaagaaa atccacggcc agagcacccc attgactaca
   102841 ctactccaga acacttccgg tctgaaagag acgttaatca ctaaaaactc tgcccagaaa
   102901 acaataatat ccaccaccag cgaaagcaac atgacgacca gcgcctgctg ccataatgcg
   102961 aggttgcgga aaagctggta tttcagcgcc accaggtaag caatgatgct catcgccaat
   103021 acgcgtacgc caagcgtcga gccgctgatc agatccagta tggcacccat cacaaaacct
   103081 gtgcccacat ttacgcgatg aggcaaggcc aggatccaat acaacaagat gagtaacacc
   103141 cagtttggcc ggaaaacaat caggttatcc ggccagggca tgatttgcag caacagcgca
   103201 atgaggaaag agagccagat tacccaacgt ccctggctac gatagctcgc cactattgcc
   103261 ctcccggcgc acgcgcaggc ggttgagcac cactttgcgg cggcgtagcc ctttgtggag
   103321 agcgattagc agcaggctgt gtgggcgcag caggcgcagt agctgcattt cctgtcgtcg
   103381 gttgctgcgg agtcggctga gcgatccccg ttgccggttc aggtaacttt ggccccatcg
   103441 cgtctggcga aggcaatacc tgcggcatca tctgcatcag acgttcatta gcaacacgat
   103501 gcacctcttc cggcgtcatc gggttagcgc cgttacgatc cgcgccccac agcagcagca
   103561 gataacgcaa acgttgcagt cctgcagtcg gacgcgcctg aatcacagta taagcgcgct
   103621 gggtatcgag ttttacggaa gagacaaccg cgaccggata gccttccggg aaacgaccgc
   103681 ccagaccgga ggtcaccagc acatcaccaa cacgaatatc cgtattcgct ggcagatgct
   103741 caagctgcaa atcatccgta caaccgttac cggctgcaat tacgcggata tcgttgcgca
   103801 gcacctggat tggcagcgcg tgggtcgcat cacaaatcag cagcacgcga ctggtcagtt
   103861 tagcgacggc caccacctga ccaacaacac ctttgtcgct gatgaccggc tggccttcat
   103921 aaacgccatt aacgctacct ttatcgataa caacttgatc gctataagga tcgttaaccg
   103981 tggagataac ctgagtcacc attttctgct catcctgacg cagcggggaa cccagcagct
   104041 cgcgcagacg cgcgttctcc tgtttgtatt gtccaagcat cagcagttca ctgtttttca
   104101 gcaacagttc ctgacgtaac gcccggtttt caagttctag ttggtcacgc gaggccagcg
   104161 tctgcgatac gccatccagc aattcacgag gagcattgga aacaaagtag aaaggactga
   104221 cggcggtatc catataagta cggatttgac tgaacgtccc caggcggctg tcggcaataa
   104281 taatgccgag cgccaccagc accgccagaa taaggcgaat ctgtagcgac gggccacggc
   104341 taaaaattgg cttcataagt tatgcgtatt ctcgtatcag accaggcagg gtaaacagac
   104401 atttcccctg cctgcatccg attactcttc gctgaacagg tcgccgccgt gcatgtcgat
   104461 catttccagc gctttgccgc caccgcgcgc cacacaggtc agcgggtctt cagcaacaac
   104521 gactggaatg ccggtttctt ccattaacaa acggtcaagg ttacgcagca gtgcgccacc
   104581 accggtgagc accatgccgc gctcggagat gtcggaagcc agttccggcg ggcactgttc
   104641 cagtgcaacc attaccgcgc tcacaatacc ggtcagcggt tcctgcagtg cttcgaggat
   104701 ttcgttggag ttcagggtaa aaccgcgtgg aacaccttct gccaggttac ggccacgaac
   104761 ttcgatttca cggacttcat cgcccgggta agccgaaccg atttcgtgct tgatacgttc
   104821 tgcggtggct tcaccgatca gagaaccgta attacgacgc acatagttga tgatagcttc
   104881 gtcgaaacga tcaccaccaa tgcgcacgga agaggagtaa accacgccgt tcaaggagat
   104941 aacagcaact tcagtggtac caccaccgat atcaaccacc atagaaccgg tcgcttcaga
   105001 aaccggcagg ccagcaccaa ttgcggcagc catcggttct tcaatcagga agacttcacg
   105061 ggcaccagcg ccctgcgcgg attcacgaat tgcgcggcgt tcaacctggg tcgcgccaac
   105121 cggtacacaa accagaacgc gcgggcttgg acgcataaag ctgttgctgt gcacttgttt
   105181 gatgaagtgc tggagcattt tttcagtcac gaagaagtcg gcgataacgc cgtctttcat
   105241 tgggcgaatg gcagcaatat tgcccggcgt acggcccagc atctgcttcg cgtcatgacc
   105301 tactgcagct acgcttttcg gtgaaccggc acgatcctga cgaatggcca ccacggaagg
   105361 ctcattcaat acgatgcctt gtccttttac ataaatgagg gtattcgcag tacccaggtc
   105421 aatggacaag tcattggaaa acatgccacg aaattttttc aacatactaa gggataatcc
   105481 tgaaagctgg ggcggaaaag aaaatccgct tactttacca accacacgca gcagcgacaa
   105541 ggcgcaaaaa tcatctgcta cggtgaaaat tagtgcagtt cgtttccttt gttacaaatc
   105601 tctgcctgag tccagaaagg cttaatgcat cagcagcatt cctcgcctgt ttgcaaccgc
   105661 gtaaggtcat tcatctgcat atgtgctgca acaatctggc gagcagacaa gcacactccc
   105721 atgagacgca gcgcgcgtta ttctacgtga aaacagatta aacggcaggt taaaccgagt
   105781 atctttgtga atattttttc acgttagtat caagtggctg tgaggacgcg aaaaaatccc
   105841 cttgcccgcc tgtaacaccg cgctgaatca gggtctgcca ctcgcttcgc gaacgcacac
   105901 cggtggcgta aacttgggtg ctggtcccgg agcaggcttc caccaggctt tgaaccagca
   105961 gctggttctc cgttcgcttc tcaatgtttc tgaccagccc cggatggagc ttgagtaact
   106021 caacattaag ttctttgatc caactggtgc ttaccagcgt caaaccagcc tggttgacgg
   106081 ctacccgtac ccctaaagca ttcactaaac gaataacagg ttgtaaacga ctgatatgtt
   106141 gacctacatc ggcctctgca agttcaataa ttatgcgttt tcgttgtgat ttttcacatt
   106201 gcattaacgt atcgcgcagc caacgctgaa aacgcgggcg aatcagcgac tcaacggtaa
   106261 cctgaatcgc cagattttcc tctggccagt aacgcaatag tggaataaga cggctgattt
   106321 gcagacggtc atactcttcc gataagccaa actgcaagac catcggcata tactccgccg
   106381 agctaacctc ttcattacca tcgaagatgc ggcacatgag ttcgcgatga tgaacctgac
   106441 cttcgcgagt aaccgccggt ttttgataaa ggcgcgggcc gccgcgactg agcatttgct
   106501 cgataagcgt acgccagcga acattaccgc gtcctttttc aggcaacgag tcatcgtaaa
   106561 tagcccagct attgccgccc tgcaatcccg cattacgcgt ggcagactct gcatgttcca
   106621 ttacctgctc ggtatcctga ccactacgcc aggcgcagat accaatgtgg atcatatcgt
   106681 cgcgatcgag cattttattg ttcggcaagg tatcgacggc tttgattaac tgaccggcga
   106741 tgctctctgc ctcttttaac gtccggtgcg gtaacagcgc agcaaaatca ctgcggtggt
   106801 aacgcgccag cagtgcgcca gggtagcgca tcataaatgt cgacagcaga ttcgtcagag
   106861 tgaagaactg ttcttcaacc tggctgtgcc cccaggtatc gctcaacata ttgaaatccg
   106921 gcagacgaat catcatcacg atcccgtggg tacctacttt ctcctgatct tccagtaacg
   106981 ttgctaactg attatcgaaa aagagtcggt tattgaggcc ggttttcacg tcctgggcgg
   107041 cataagagcg gatcagcgta tcaagacggc tgtgttgttc gcgtgcgttc tgaatttcac
   107101 gaagcagcgt atctagcgca ctgctggttc tgggcggcca ttcatagatg gttcccaaca
   107161 cattagagcc acgctcaccg tttaagatac gagtagcccg ggtttccagc aattcttgcc
   107221 cggcaagttg ccgttgtaac cagcgtaccg ccaggaagag cataacaatg ataaagccaa
   107281 tcgcccccgt gagcggcgcg gtagtcatta aagaatggaa atagttgccc atcggatcct
   107341 gataaaccag acgcagcgac atccccggat gctttatcaa cggaacactc agttcgcgaa
   107401 acagatcgct ggagccaacc ggacgataac taccatttct ggccagggta taaacctgtt
   107461 tatcaccatg gagcaggtct acacgaacga tatctgccga catcattaat tcggtaattt
   107521 gtggccttaa tgtgctgaag tcattcgaca caaggtgggt atcaatcgcc gtcgccaccg
   107581 cctgaacgcg atgactaaac ttatactgaa tggcgttgta gaaacttagc gaacagccca
   107641 gcaaagtcac aaaaattgtt aacccggtga gcagcgtaac aaaggccgaa aatttcgtcg
   107701 ttaatctcat ccttgtgtta actccgataa tgaggaagcg ggcatactag caaatcagat
   107761 ttatctcgca atttattgcg cttcatcggc tttgcttttc cattagcgag tatagtcttc
   107821 agaaattatt ttccaatcca tcatgcacat gaggaccact tatgcaggcg ttacttttag
   107881 aacagcagga cggcaaaact ctcgcatcgg tacagacact tgacgaaagt cgcctgccgg
   107941 agggcgatgt cacggttgat gttcactggt cgagcctgaa ctataaagat gcgctggcga
   108001 ttaccggtaa gggaaaaatc atccgtaatt ttccgatgat tcctgggatc gattttgccg
   108061 gaactgtacg caccagcgaa gatccgcgtt ttcatgccgg tcaggaggtg ttactcactg
   108121 gctggggcgt tggtgaaaac cactggggtg ggctggcgga gcaggcgcga gtgaaaggtg
   108181 actggctggt tgccatgccg caagggctgg acgcgcgtaa agcaatgatt atcggtactg
   108241 ccggttttac cgccatgctg tgtgtgatgg cgctggaaga tgccggtgtt cgcccgcagg
   108301 acggggagat tgtcgtgacg ggtgccagtg gtggcgtcgg cagtaccgcc gtggcgctgc
   108361 tgcataagtt gggttatcag gtcgttgccg tttccggtcg cgaaagtacc catgaatatc
   108421 tgaaaagttt aggtgccagt cgtattctcc ctcgtgatga gtttgccgaa tcccgtcctc
   108481 tggaaaaaca agtctgggct ggggcaattg acactgttgg cgacaaagtg ctggcaaaag
   108541 tgctggcgca aatgaattac ggcggctgcg tggctgcctg tggtctggcg ggtggtttta
   108601 ctctgccaac cacggtcatg ccatttattc tgcgtaatgt ccgtttgcaa ggggtggatt
   108661 cagtaatgac gccaccagaa cgccgcgcac aagcctggca gcgactggtc gccgatttac
   108721 cggaatcatt ctatacccag gcggcaaaag agatatctct gtcagaggca ccgaactttg
   108781 ccgaggccat cattaataac cagatccagg gtcgcacgct ggtgaaggtt aactaaccat
   108841 ttagcaagga ataataagag agggaactca ttttgacatc attattcaat cggttaacgg
   108901 gaaaagcggt tagccggaca gctttcgtcg aacaccttgg tcaggaagtt gtacaacatc
   108961 atccaaactg gaaagtcatg atttcgactg accacaaatt gatgcgcatt gatactcctc
   109021 taaacagcca ttattgatat gcctccgtcg cctgttaggt ttatggtgct ttgcctgggc
   109081 gacactacgc ttaacctgtt actgatttct ggtaccatgg ggtgaataat ctgattttat
   109141 ttgactacaa attaatcact cgaacctatt taatgctgag cattgtcaat cggttaattt
   109201 tgcgtgcttt agcattaaca tctatccaga caatgcagtg aaaattgggt aatccccagc
   109261 aaccgctgcg taatgtcgtc tatcttgtcg cgatcctggc atccctacat tatttgtggt
   109321 ctgtgaagat tatctcattg cagcccctca tcttcgcagg gctggcttta cagcttttca
   109381 ccttacgtta taagaagttc cgtcgatgat ggcgctaatt tcgtgaattg tgcggcttgt
   109441 tgcaaattac acggtgttga aggttattta catgttagct gttgattatc ttccctgata
   109501 agaccagtat ttagctgcca attgctacga aatcgttata atgtgcgacc tcgtcctccc
   109561 tgacgcagtt tttgcgctgc ggaaaaggtg acattggcgc aacgaaggta tattttgttt
   109621 tttgccggag gatagcagca gatcgctgca caatgtccgt caagtctaac attgacactc
   109681 tggggcaaaa tagaccggcg tcccggcctg ctggaattta tcgctatgca tacagctgtc
   109741 ggggcatacg ctttacagac ggcggtgaaa cgcctgtcac aatcacacta aacaaagagt
   109801 acggaaccca ctcatggata ttcgtaagat taaaaaactg atcgagctgg ttgaagaatc
   109861 aggcatctcc gaactggaaa tttctgaagg cgaagagtca gtacgcatta gccgtgcagc
   109921 tcctgccgca agtttccctg tgatgcaaca agcttacgct gcaccaatga tgcagcagcc
   109981 agctcaatct aacgcagccg ctccggcgac cgttccttcc atggaagcgc cagcagcagc
   110041 ggaaatcagt ggtcacatcg tacgttcccc gatggttggt actttctacc gcaccccaag
   110101 cccggacgca aaagcgttca tcgaagtggg tcagaaagtc aacgtgggcg ataccctgtg
   110161 catcgttgaa gccatgaaaa tgatgaacca gatcgaagcg gacaaatccg gtaccgtgaa
   110221 agcaattctg gtcgaaagtg gacaaccggt agaatttgac gagccgctgg tcgtcatcga
   110281 gtaacgaggc gaacatgctg gataaaattg ttattgccaa ccgcggcgag attgcattgc
   110341 gtattcttcg tgcctgtaaa gaactgggca tcaagactgt cgctgtgcac tccagcgcgg
   110401 atcgcgatct aaaacacgta ttactggcag atgaaacggt ctgtattggc cctgctccgt
   110461 cagtaaaaag ttatctgaac atcccggcaa tcatcagcgc cgctgaaatc accggcgcag
   110521 tagcaatcca tccgggttac ggcttcctct ccgagaacgc caactttgcc gagcaggttg
   110581 aacgctccgg ctttatcttc atcggcccga aagcagaaac cattcgcctg atgggcgaca
   110641 aagtatccgc aatcgcggcg atgaaaaaag cgggcgttcc ttgcgtacct ggttctgacg
   110701 gcccgctggg cgacgatatg gataaaaacc gcgccattgc taaacgcatt ggttatccgg
   110761 tgattatcaa agcctccggc ggcggcggcg gtcgcggtat gcgcgtagtg cgcggcgacg
   110821 ctgaactggc acaatccatc tccatgaccc gtgcggaagc gaaagctgct ttcagcaacg
   110881 atatggttta catggagaaa tacctggaaa atcctcgcca cgtcgagatt caggtactgg
   110941 ctgacggtca gggcaacgct atctatctgg cggaacgtga ctgctccatg cagcgccgcc
   111001 accagaaagt ggtcgaagaa gcgccagcac cgggcattac cccggaactg cgtcgctaca
   111061 tcggcgaacg ttgcgctaaa gcgtgtgttg atatcggcta tcgcggtgca ggtactttcg
   111121 agttcctgtt cgaaaacggc gagttctatt tcatcgaaat gaacacccgt attcaggtag
   111181 aacacccggt taccgaaatg atcaccggcg ttgacctgat caaagaacag ctgcgtatcg
   111241 ctgccggtca accgctgtcg atcaagcaag aagaagttca cgttcgcggc catgcggtgg
   111301 aatgtcgtat caacgccgaa gatccgaaca ccttcctgcc aagcccaggc aaaatcaccc
   111361 gtttccacgc acctggcggt tttggcgtac gttgggagtc tcatatctac gcgggctaca
   111421 ccgtaccgcc gtactatgac tcaatgatcg gtaagctgat ttgctacggt gaaaaccgtg
   111481 acgtggcgat tgcccgcatg aagaatgcgc tgcaggagct gatcatcgac ggtatcaaaa
   111541 ccaatgttga tctgcagatc cgcatcatga atgacgagaa cttccagcat ggtggcacta
   111601 acatccacta tctggagaaa aaactcggtc ttcaggaaaa ataagactgc aaaagcgtca
   111661 aaaggccgga ttttccggcc ttttttatta ctggggatcg acaaccccca taaggtacaa
   111721 tcctcgcttt cttcacccat cagggacaaa aaatggacac tcgttttgtt caggcccata
   111781 aagaggcgcg ctgggcgctg gggctgaccc ttttgtatct ggcagtttgg ttagtagccg
   111841 cttacttacc tggcgttgcc cccggtttta ccggctttcc gcgctggttt gagatggcct
   111901 gcatcctgac gccgctactg tttattggac tgtgctgggc gatggtgaaa tttatctatc
   111961 gcgatatccc actggaggat gacgatgcag cttgaagtaa ttctaccgct ggtcgcctat
   112021 ctggtggtgg tgttcggtat ctcggtttat gcgatgcgta aacggagcac cggcaccttc
   112081 cttaatgagt atttcctcgg cagccgctct atgggcggta ttgtgctggc gatgacgctc
   112141 accgcgacct atatcagtgc cagttcgttt atcggcgggc ctggagctgc ttataaatac
   112201 ggcctgggtt gggtattgct ggcgatgatt cagcttcctg cagtctggct ttcgctcggt
   112261 attctcggca agaagttcgc gattcttgcg cgccgctaca atgcagtgac gctgaacgat
   112321 atgctgtttg cccgctacca gagtcgtctt ctggtgtggc tggcgagttt gagtttgctg
   112381 gttgcgttcg ttggggcaat gaccgtgcag tttatcggcg gtgcgcgcct gctggaaacc
   112441 gcggcgggta ttccttatga aaccgggctg ctgattttcg gtatcagcat tgcgttgtat
   112501 accgcctttg gcggctttcg cgccagcgtg ctgaacgaca ccatgcaagg gcttgtgatg
   112561 ctgattggca ccgttgtgct gcttattggc gtggtacatg ccgctggcgg cttaagcaac
   112621 gcagtacaga ccttgcaaac catcgatccg caactggtta cgccacaagg cgctgacgat
   112681 attctgtcgc ctgcctttat gacgtcgttc tgggtactgg tgtgctttgg cgtgattggc
   112741 ctgccgcata ctgcggtgcg ctgtatctct tataaagaca gcaaagcagt acaccggggg
   112801 atcatcatcg gtacgattgt agtcgcaatc ctgatgttcg gtatgcacct ggccggagcg
   112861 ttaggtcgtg cagtgatccc cgatctcacc gtaccggatc tggtgatccc gacgttaatg
   112921 gtaaaagtgc tgccaccgtt tgctgccggg atctttctgg ctgcacctat ggctgcgatc
   112981 atgtcgacaa ttaacgccca attgctacaa agttccgcta cgatcattaa agatctctat
   113041 ctgaatatcc gtccggatca aatgcaaaac gagacgcgtc tgaagcggat gtcggcggta
   113101 attacgttag ttctcggcgc gttgctgctg cttgccgcct ggaagccgcc agaaatgatc
   113161 atctggctga atttgctggc cttcggtggg ctggaagccg ttttcctgtg gccgctggtg
   113221 ctgggtcttt actgggaacg cgccaacgcc aaaggcgcgc taagtgcgat gatcgttggc
   113281 ggcgtgctgt atgccgtact cgcgacgctg aatattcagt acctgggctt ccaccctatc
   113341 gtgccctcgt tactactaag tttggtggct ttcctggtcg gaaaccgttt cggtacatcc
   113401 gtcccgcaag ctaccgtttt gactactgat aaataaagag ttttgccatg ccttggatcc
   113461 aactgaaact gaacaccacc ggcgcgaacg cggaagatct tagcgatgcg ctgatggaag
   113521 cgggtgccgt ttctatcact tttcaggata cccacgatac gccagtattt gagccgctgc
   113581 cgggcgaaac gcgcctgtgg ggcgacaccg atgtgattgg tctgttcgac gctgaaaccg
   113641 atatgaacga cgtggtggcg attctggaaa accatccgct gctcggcgca ggcttcgcgc
   113701 ataaaatcga acaactagaa gataaagact gggagcgcga atggatggat aatttccacc
   113761 cgatgcgctt tggtgaacga ctgtggatct gccctagctg gcgtgatgtg ccggacgaaa
   113821 acgccgtcaa cgtgatgtta gatccagggc tggcgtttgg tacgggtacc catccaacca
   113881 cctctctgtg cctgcaatgg ctcgacagcc tcgatttaac cggtaaaaca gtcatcgact
   113941 ttggctgtgg ttccggcatt ctggcgatcg cggcgctgaa actgggtgca gcaaaagcca
   114001 ttggtattga tatcgatccg caggcgattc aggccagccg cgacaacgcc gaacgtaatg
   114061 gcgtttctga ccgtctggaa ctctacttac cgaaagatca gccagaagaa atgaaagccg
   114121 acgtggtggt cgctaacatc cttgcaggcc cattacgtga actggcaccg ttaatcagcg
   114181 tcctgccggt ttcaggcggt ttgctgggcc tttccggtat tctggcaagc caggcagaga
   114241 gcgtttgtga agcttatgtc gacagcttcg cactggaccc ggtcgtggaa aaagaagagt
   114301 ggtgccgcat taccggtcgt aagaattaac cttcgcatcg ccgtagggtg acgcggggcg
   114361 aagtgcgagc aagctcacaa aaggcacgta aatttgccga ttatttacgc aaatttgcgt
   114421 gccaaaattt tcattcataa agaaaaattg agaacttact caaatttctt tgagtgtaaa
   114481 ttttagtcac tattttctaa tatgatgatt tttatgagta attatcgcac cacgctcatt
   114541 ttaaatgcaa ttctttgatc catctcagag gattggtcaa agtttggcct ttcatctcgt
   114601 gcaaaaaatg cgtaatatac gccgccttgc agtcacagta tggtcatttc ttaactcatg
   114661 cgcatcggac aatatcagct cagaaatcgc ctgatcgcag cgcccatggc tggcattaca
   114721 gacagacctt ttcggacgtt gtgctacgag atgggagccg gattgacagt atccgagatg
   114781 atgtcttcta acccacaggt ttgggaaagc gacaaatctc gtttacggat ggtgcacatt
   114841 gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg atccgaaaga aatggcagat
   114901 gcagcacgta ttaacgtgga aagcggtgcc cagattattg atatcaatat gggttgcccg
   114961 gctaaaaaag tgaatcgcaa gctcgcaggt tcagcccttt tgcagtaccc ggatgtcgtt
   115021 aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc ctgttaccct gaagattcgc
   115081 accggctggg caccggaaca ccgtaactgc gaagagattg cccaactggc tgaagactgt
   115141 ggcattcagg ctctgaccat tcatggccgt acacgcgcct gtttgttcaa tggagaagct
   115201 gagtacgaca gtattcgggc agttaagcag aaagtttcca ttccggttat cgcgaatggc
   115261 gacattactg acccgcttaa agccagagct gtgctcgact atacaggggc ggatgccctg
   115321 atgataggcc gcgcagctca gggaagaccc tggatctttc gggaaatcca gcattatctg
   115381 gacactgggg agttgctgcc cccgctgcct ttggcagagg ttaagcgctt gctttgcgcg
   115441 cacgttcggg aactgcatga cttttatggt ccggcaaaag ggtaccgaat tgcacgtaaa
   115501 cacgtttcct ggtatctcca ggaacacgct ccaaatgacc agtttcggcg cacattcaac
   115561 gccattgagg atgccagcga acagctggag gcgttggagg catacttcga aaattttgcg
   115621 taaacagaaa taaagagctg acagaactat gttcgaacaa cgcgtaaatt ctgacgtact
   115681 gaccgtttct accgttaact ctcaggatca ggtaacccaa aaacccctgc gtgactcggt
   115741 taaacaggca ctgaagaact attttgctca actgaatggt caggatgtga atgacctcta
   115801 tgagctggta ctggctgaag tagaacagcc cctgttggac atggtgatgc aatacacccg
   115861 tggtaaccag acccgtgctg cgctgatgat gggcatcaac cgtggtacgc tgcgtaaaaa
   115921 attgaaaaaa tacggcatga actaattcag gttagctaaa tgcttgatta aaaaggcgct
   115981 actcggcatg gggaagcgcc ttttttatag gtgtcacaaa gggagtgacc atgagaacag
   116041 gatgtgaacc gacccggttt ggtaatgaag ctaagaccat tattcacggt gatgcccttg
   116101 ccgaacttaa aaagctacct actgaaagcg tcgatctgat ctttgccgac ccaccgtata
   116161 acatcggtaa aaattttgat ggtctgatcg aagcctggaa agaagatctg tttatcgact
   116221 ggctgtttga agtgattgca gagtgccacc gcgttctgaa aaagcagggc agcatgtaca
   116281 ttatgaacag tacggaaaac atgcccttta tcgatctcca gtgccgcaag ctttttacca
   116341 tcaaaagtcg catcgtctgg tcatatgaca gttctggagt acaggcgaaa aaacactacg
   116401 gctccatgta cgaacccatc ctgatgatgg tgaaagacgc aaagaactac acattcaacg
   116461 gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc gattatcgca
   116521 aaaatcctcc acagccatac aatcatcaaa aagtaccggg taacgtctgg gattttccgc
   116581 gcgtgcgtta tttaatggat gaatatgaga accacccgac gcaaaaaccg gaagccttac
   116641 tgaaacgcat tattctcgcc tcttccaacc caggcgatat cgttctcgac ccgtttgccg
   116701 gtagctttac taccggtgcc gtagccgtct ccagcgggcg aaaattcatt ggtattgaga
   116761 tcaacagcga gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg cattactctg
   116821 cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa cctgtcaaaa cgaagccggt
   116881 taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct ggttttcaga
   116941 ttattcattt cgtgtatatt tcctgccaga cttggttaaa catgcacagg ctcaggtaat
   117001 gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt ttgatacttt
   117061 actgatgcag tggattgaac tgctgacaac agaaacagac aaatgccgca atatgaactc
   117121 agttaatcca ctaaaactgg ttaactgtga cgaactgaat tttcaggaca gaatgtgaat
   117181 ttacatgaca cttaattcat tcgtttgatg aattaatttc gttatgtttt catctggcat
   117241 gaacattctt aatacgttat cgaccagagc gggggcttgt ttataaagat cataacccgc
   117301 catattcatt aaccagtttt gaacaattcc gctgaaggca ccattaataa taatcatcac
   117361 aacatctaaa tcgaggttat ttgctacaca accttgttgc tgacacgcct gcaatacttc
   117421 gcggagagtc tgcggattga agcccatctt ttcgcgtatc actccctcgg ccagcatctc
   117481 atcattaaat tcacatttgt gatataagat tttcagcaac gcctgctggc ggggaatttt
   117541 ggcaatatat tgcaagccga caatcaattt ttcacgcaat tgttgaaatg ggtcatgctc
   117601 taacccagcc gtcaagtgat cctggattaa ctctcgcaat gaaggctgtt gcaaccacat
   117661 ctcattaaac agttgagtct tgttttcgaa gtgccagtag atagcgccac gcgtaacgtt
   117721 agcagcgtcg gcaatgtcgt tgagcgtcgt cttgcttacg ccatgctgcg caaactgggc
   117781 gatggcagtt tcaatcagtt cttgccgggt cttcagagct tcggctttgg ttctttttgc
   117841 catgattaat tattcaggaa ataaatatat tcgacacaga gtgagaaaat agcgaaggtt
   117901 aatctatcac ctaatgtgta tttatatgag aggctaatat tgagttgcta taaatcgtta
   117961 aataaataat ata
//
LOCUS       DAZFFI010000003       115824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFFI010000003 DAZFFI010000000
VERSION     DAZFFI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 115824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.003"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..440)
                     /locus_tag="ACHJ12_000243"
     CDS             complement(<1..440)
                     /locus_tag="ACHJ12_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4988094.1"
                     /translation="MKKAPNLKLQPKDKMTEVIIFAGSDAWAHAKEWSEWAGKHIAAD
                     DTPPVILGPKQLASLEDTKIIDKGRNYVRVYRAGEISETALLQIATLLAVAGVKEARC
                     YSGFVDQQPEDWTPRMAGLKDDAERGNSLVINLPAKTNFTGNYD"
     gene            complement(437..732)
                     /locus_tag="ACHJ12_000244"
                     /pseudo
     CDS             complement(437..732)
                     /locus_tag="ACHJ12_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(790..993)
                     /locus_tag="ACHJ12_000245"
     CDS             complement(790..993)
                     /locus_tag="ACHJ12_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988095.1"
                     /translation="MQEMTLAEASLKAQQIEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            complement(993..1178)
                     /locus_tag="ACHJ12_000246"
     CDS             complement(993..1178)
                     /locus_tag="ACHJ12_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988096.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVHEIKGVQ"
     gene            complement(1171..1449)
                     /locus_tag="ACHJ12_000247"
     CDS             complement(1171..1449)
                     /locus_tag="ACHJ12_000247"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4988097.1"
                     /translation="MQTRLKRIAVLSQPLTTNDSKSIEAAMRNHITHPQGRDSHNLNK
                     FTWRFMQCLTDSIAIFTVTAATEHEARAQLPHAHLIFVARIRQEVTHA"
     gene            1568..1747
                     /locus_tag="ACHJ12_000248"
     CDS             1568..1747
                     /locus_tag="ACHJ12_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4988098.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            1805..2041
                     /locus_tag="ACHJ12_000249"
     CDS             1805..2041
                     /locus_tag="ACHJ12_000249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988099.1"
                     /translation="MNKKYQSITDIFAEQRRQLLHRILERQLELKRAKEISEPEELTP
                     LPGVKKHPQKRFALEDFVEKYPRRLKATKNRPPG"
     gene            complement(2621..2824)
                     /locus_tag="ACHJ12_000250"
     CDS             complement(2621..2824)
                     /locus_tag="ACHJ12_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4988100.1"
                     /translation="MANNNSFIRLSEVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSV
                     AFIESEVDEWINQRIDASRNNAA"
     gene            complement(3368..4582)
                     /locus_tag="ACHJ12_000251"
     CDS             complement(3368..4582)
                     /locus_tag="ACHJ12_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4988101.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGVCA"
     gene            complement(4747..4820)
                     /locus_tag="ACHJ12_000252"
     tRNA            complement(4747..4820)
                     /locus_tag="ACHJ12_000252"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4786..4788),aa:Gly,seq:ccc)
     gene            complement(4899..5654)
                     /gene="actS"
                     /locus_tag="ACHJ12_000253"
     CDS             complement(4899..5654)
                     /gene="actS"
                     /locus_tag="ACHJ12_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4988102.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6069..8366
                     /gene="xdhA"
                     /locus_tag="ACHJ12_000254"
     CDS             6069..8366
                     /gene="xdhA"
                     /locus_tag="ACHJ12_000254"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4988103.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     DRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            8377..9255
                     /gene="xdhB"
                     /locus_tag="ACHJ12_000255"
     CDS             8377..9255
                     /gene="xdhB"
                     /locus_tag="ACHJ12_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4988104.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            9252..9731
                     /gene="xdhC"
                     /locus_tag="ACHJ12_000256"
     CDS             9252..9731
                     /gene="xdhC"
                     /locus_tag="ACHJ12_000256"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4988105.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(9771..11549)
                     /gene="ygeV"
                     /locus_tag="ACHJ12_000257"
     CDS             complement(9771..11549)
                     /gene="ygeV"
                     /locus_tag="ACHJ12_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4988106.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            12028..13215
                     /gene="ygeW"
                     /locus_tag="ACHJ12_000258"
     CDS             12028..13215
                     /gene="ygeW"
                     /locus_tag="ACHJ12_000258"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4988107.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            13273..14469
                     /gene="dpaL"
                     /locus_tag="ACHJ12_000259"
     CDS             13273..14469
                     /gene="dpaL"
                     /locus_tag="ACHJ12_000259"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4988108.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            14527..15738
                     /gene="ygeY"
                     /locus_tag="ACHJ12_000260"
     CDS             14527..15738
                     /gene="ygeY"
                     /locus_tag="ACHJ12_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4988109.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            15791..17176
                     /gene="hyuA"
                     /locus_tag="ACHJ12_000261"
     CDS             15791..17176
                     /gene="hyuA"
                     /locus_tag="ACHJ12_000261"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4988110.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            17224..18156
                     /gene="arcC"
                     /locus_tag="ACHJ12_000262"
     CDS             17224..18156
                     /gene="arcC"
                     /locus_tag="ACHJ12_000262"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4988111.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(18197..19822)
                     /gene="yqeB"
                     /locus_tag="ACHJ12_000263"
     CDS             complement(18197..19822)
                     /gene="yqeB"
                     /locus_tag="ACHJ12_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4988112.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(19870..20640)
                     /gene="yqeC"
                     /locus_tag="ACHJ12_000264"
     CDS             complement(19870..20640)
                     /gene="yqeC"
                     /locus_tag="ACHJ12_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4988113.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            20743..21321
                     /gene="mocA"
                     /locus_tag="ACHJ12_000265"
     CDS             20743..21321
                     /gene="mocA"
                     /locus_tag="ACHJ12_000265"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4988114.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDDAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            21643..24741
                     /gene="ygfK"
                     /locus_tag="ACHJ12_000266"
     CDS             21643..24741
                     /gene="ygfK"
                     /locus_tag="ACHJ12_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4988115.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            24744..26072
                     /gene="ssnA"
                     /locus_tag="ACHJ12_000267"
     CDS             24744..26072
                     /gene="ssnA"
                     /locus_tag="ACHJ12_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4988116.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            26123..26902
                     /gene="ygfM"
                     /locus_tag="ACHJ12_000268"
     CDS             26123..26902
                     /gene="ygfM"
                     /locus_tag="ACHJ12_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4988117.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAEEEAV"
     gene            26899..29769
                     /gene="xdhD"
                     /locus_tag="ACHJ12_000269"
     CDS             26899..29769
                     /gene="xdhD"
                     /locus_tag="ACHJ12_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4988118.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKVLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAISHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            29934..31334
                     /gene="xanQ"
                     /locus_tag="ACHJ12_000270"
     CDS             29934..31334
                     /gene="xanQ"
                     /locus_tag="ACHJ12_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4988119.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            31352..32668
                     /gene="guaD"
                     /locus_tag="ACHJ12_000271"
     CDS             31352..32668
                     /gene="guaD"
                     /locus_tag="ACHJ12_000271"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4988120.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            32704..34071
                     /gene="ghxQ"
                     /locus_tag="ACHJ12_000272"
                     /gene_synonym="ygfQ"
     CDS             32704..34071
                     /gene="ghxQ"
                     /locus_tag="ACHJ12_000272"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4988121.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(34107..34595)
                     /gene="ygfS"
                     /locus_tag="ACHJ12_000273"
     CDS             complement(34107..34595)
                     /gene="ygfS"
                     /locus_tag="ACHJ12_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4988122.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(34595..36514)
                     /gene="ygfT"
                     /locus_tag="ACHJ12_000274"
     CDS             complement(34595..36514)
                     /gene="ygfT"
                     /locus_tag="ACHJ12_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4988123.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            36950..38398
                     /gene="uacT"
                     /locus_tag="ACHJ12_000275"
     CDS             36950..38398
                     /gene="uacT"
                     /locus_tag="ACHJ12_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4988124.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            38400..38525
                     /gene="yqfG"
                     /locus_tag="ACHJ12_000276"
     CDS             38400..38525
                     /gene="yqfG"
                     /locus_tag="ACHJ12_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4988125.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(38522..38593)
                     /locus_tag="ACHJ12_000277"
     CDS             complement(38522..38593)
                     /locus_tag="ACHJ12_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988126.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            38648..39196
                     /gene="idi"
                     /locus_tag="ACHJ12_000278"
     CDS             38648..39196
                     /gene="idi"
                     /locus_tag="ACHJ12_000278"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4988127.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            complement(39239..40756)
                     /gene="lysS"
                     /locus_tag="ACHJ12_000279"
     CDS             complement(39239..40756)
                     /gene="lysS"
                     /locus_tag="ACHJ12_000279"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4988128.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(40766..41864)
                     /gene="prfB"
                     /locus_tag="ACHJ12_000280"
     CDS             complement(join(40766..41788,41790..41864))
                     /gene="prfB"
                     /locus_tag="ACHJ12_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4988129.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(41955..43688)
                     /gene="recJ"
                     /locus_tag="ACHJ12_000281"
     CDS             complement(41955..43688)
                     /gene="recJ"
                     /locus_tag="ACHJ12_000281"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4988130.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(43694..44404)
                     /gene="dsbC"
                     /locus_tag="ACHJ12_000282"
     CDS             complement(43694..44404)
                     /gene="dsbC"
                     /locus_tag="ACHJ12_000282"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4988131.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(44429..45325)
                     /gene="xerD"
                     /locus_tag="ACHJ12_000283"
     CDS             complement(44429..45325)
                     /gene="xerD"
                     /locus_tag="ACHJ12_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4988132.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            45437..45958
                     /gene="fldB"
                     /locus_tag="ACHJ12_000284"
     CDS             45437..45958
                     /gene="fldB"
                     /locus_tag="ACHJ12_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4988133.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(45998..46405)
                     /gene="ygfX"
                     /locus_tag="ACHJ12_000285"
     CDS             complement(45998..46405)
                     /gene="ygfX"
                     /locus_tag="ACHJ12_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4988134.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(46386..46652)
                     /gene="sdhE"
                     /locus_tag="ACHJ12_000286"
     CDS             complement(46386..46652)
                     /gene="sdhE"
                     /locus_tag="ACHJ12_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4988135.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            46895..47875
                     /gene="ygfZ"
                     /locus_tag="ACHJ12_000287"
     CDS             46895..47875
                     /gene="ygfZ"
                     /locus_tag="ACHJ12_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4988136.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(48071..48730)
                     /gene="yqfA"
                     /locus_tag="ACHJ12_000288"
     CDS             complement(48071..48730)
                     /gene="yqfA"
                     /locus_tag="ACHJ12_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4988137.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(48894..49205)
                     /gene="yqfB"
                     /locus_tag="ACHJ12_000289"
     CDS             complement(48894..49205)
                     /gene="yqfB"
                     /locus_tag="ACHJ12_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4988138.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            49250..50683
                     /gene="bglA"
                     /locus_tag="ACHJ12_000290"
     CDS             49250..50683
                     /gene="bglA"
                     /locus_tag="ACHJ12_000290"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4988139.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(50740..51483)
                     /gene="ygfF"
                     /locus_tag="ACHJ12_000291"
     CDS             complement(50740..51483)
                     /gene="ygfF"
                     /locus_tag="ACHJ12_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4988140.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(51749..54622)
                     /gene="gcvP"
                     /locus_tag="ACHJ12_000292"
     CDS             complement(51749..54622)
                     /gene="gcvP"
                     /locus_tag="ACHJ12_000292"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4988141.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGTEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(54741..55130)
                     /gene="gcvH"
                     /locus_tag="ACHJ12_000293"
     CDS             complement(54741..55130)
                     /gene="gcvH"
                     /locus_tag="ACHJ12_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4988142.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(55154..56248)
                     /gene="gcvT"
                     /locus_tag="ACHJ12_000294"
     CDS             complement(55154..56248)
                     /gene="gcvT"
                     /locus_tag="ACHJ12_000294"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4988143.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            56539..56622
                     /locus_tag="ACHJ12_000295"
     CDS             56539..56622
                     /locus_tag="ACHJ12_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988144.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(56696..57898)
                     /gene="ubiI"
                     /locus_tag="ACHJ12_000296"
                     /gene_synonym="visC"
     CDS             complement(56696..57898)
                     /gene="ubiI"
                     /locus_tag="ACHJ12_000296"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4988145.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(57921..59099)
                     /gene="ubiH"
                     /locus_tag="ACHJ12_000297"
                     /gene_synonym="visB"
     CDS             complement(57921..59099)
                     /gene="ubiH"
                     /locus_tag="ACHJ12_000297"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4988146.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(59096..60421)
                     /gene="pepP"
                     /locus_tag="ACHJ12_000298"
     CDS             complement(59096..60421)
                     /gene="pepP"
                     /locus_tag="ACHJ12_000298"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4988147.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(60447..61025)
                     /gene="ygfB"
                     /locus_tag="ACHJ12_000299"
     CDS             complement(60447..61025)
                     /gene="ygfB"
                     /locus_tag="ACHJ12_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4988148.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            61193..61522
                     /gene="zapA"
                     /locus_tag="ACHJ12_000300"
     CDS             61193..61522
                     /gene="zapA"
                     /locus_tag="ACHJ12_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4988149.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            61564..61747
                     /gene="ssrS"
                     /locus_tag="ACHJ12_000301"
     ncRNA           61564..61747
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ12_000301"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            61768..62370
                     /gene="fau"
                     /locus_tag="ACHJ12_000302"
     CDS             61768..62370
                     /gene="fau"
                     /locus_tag="ACHJ12_000302"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4988150.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(62471..62530)
                     /locus_tag="ACHJ12_000303"
     CDS             complement(62471..62530)
                     /locus_tag="ACHJ12_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4988151.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(62863..62922)
                     /gene="ibsC"
                     /locus_tag="ACHJ12_000304"
     CDS             complement(62863..62922)
                     /gene="ibsC"
                     /locus_tag="ACHJ12_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4988152.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(63151..64383)
                     /gene="serA"
                     /locus_tag="ACHJ12_000305"
     CDS             complement(63151..64383)
                     /gene="serA"
                     /locus_tag="ACHJ12_000305"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4988153.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(64639..65298)
                     /gene="rpiA"
                     /locus_tag="ACHJ12_000306"
     CDS             complement(64639..65298)
                     /gene="rpiA"
                     /locus_tag="ACHJ12_000306"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4988154.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(65354..65584)
                     /locus_tag="ACHJ12_000307"
     CDS             complement(65354..65584)
                     /locus_tag="ACHJ12_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988155.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            65726..66619
                     /gene="argP"
                     /locus_tag="ACHJ12_000308"
     CDS             65726..66619
                     /gene="argP"
                     /locus_tag="ACHJ12_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4988156.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            66823..68967
                     /gene="scpA"
                     /locus_tag="ACHJ12_000309"
     CDS             66823..68967
                     /gene="scpA"
                     /locus_tag="ACHJ12_000309"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4988157.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            68960..69955
                     /gene="meaB"
                     /locus_tag="ACHJ12_000310"
     CDS             68960..69955
                     /gene="meaB"
                     /locus_tag="ACHJ12_000310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4988158.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRCKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            69966..70751
                     /gene="scpB"
                     /locus_tag="ACHJ12_000311"
     CDS             69966..70751
                     /gene="scpB"
                     /locus_tag="ACHJ12_000311"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4988159.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            70775..72253
                     /gene="scpC"
                     /locus_tag="ACHJ12_000312"
     CDS             70775..72253
                     /gene="scpC"
                     /locus_tag="ACHJ12_000312"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4988160.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(72250..73146)
                     /gene="ygfI"
                     /locus_tag="ACHJ12_000313"
     CDS             complement(72250..73146)
                     /gene="ygfI"
                     /locus_tag="ACHJ12_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4988161.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(73313..74053)
                     /gene="yggE"
                     /locus_tag="ACHJ12_000314"
     CDS             complement(73313..74053)
                     /gene="yggE"
                     /locus_tag="ACHJ12_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4988162.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(74146..74781)
                     /gene="argO"
                     /locus_tag="ACHJ12_000315"
     CDS             complement(74146..74781)
                     /gene="argO"
                     /locus_tag="ACHJ12_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4988163.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(74920..75780)
                     /gene="mscS"
                     /locus_tag="ACHJ12_000316"
     CDS             complement(74920..75780)
                     /gene="mscS"
                     /locus_tag="ACHJ12_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4988164.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            75976..76074
                     /locus_tag="ACHJ12_000317"
                     /pseudo
     CDS             75976..76074
                     /locus_tag="ACHJ12_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(76138..77217)
                     /gene="fbaA"
                     /locus_tag="ACHJ12_000318"
     CDS             complement(76138..77217)
                     /gene="fbaA"
                     /locus_tag="ACHJ12_000318"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4988165.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(77432..78595)
                     /gene="pgk"
                     /locus_tag="ACHJ12_000319"
     CDS             complement(77432..78595)
                     /gene="pgk"
                     /locus_tag="ACHJ12_000319"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4988166.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(78645..79664)
                     /gene="epd"
                     /locus_tag="ACHJ12_000320"
     CDS             complement(78645..79664)
                     /gene="epd"
                     /locus_tag="ACHJ12_000320"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4988167.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            80036..80467
                     /locus_tag="ACHJ12_000321"
     CDS             80036..80467
                     /locus_tag="ACHJ12_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4988168.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            80490..81068
                     /locus_tag="ACHJ12_000322"
     CDS             80490..81068
                     /locus_tag="ACHJ12_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4988169.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            81069..81776
                     /locus_tag="ACHJ12_000323"
     CDS             81069..81776
                     /locus_tag="ACHJ12_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4988170.1"
                     /translation="MHPFTSLTLWALVACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            81764..82441
                     /locus_tag="ACHJ12_000324"
     CDS             81764..82441
                     /locus_tag="ACHJ12_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4988171.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            82435..83112
                     /locus_tag="ACHJ12_000325"
     CDS             82435..83112
                     /locus_tag="ACHJ12_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4988172.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(83084..83797)
                     /locus_tag="ACHJ12_000326"
     CDS             complement(83084..83797)
                     /locus_tag="ACHJ12_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4988173.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGRGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(83794..84303)
                     /gene="fumE"
                     /locus_tag="ACHJ12_000327"
     CDS             complement(83794..84303)
                     /gene="fumE"
                     /locus_tag="ACHJ12_000327"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4988174.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(84325..85290)
                     /gene="glpX"
                     /locus_tag="ACHJ12_000328"
     CDS             complement(84325..85290)
                     /gene="glpX"
                     /locus_tag="ACHJ12_000328"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4988175.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCREMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(85287..86564)
                     /gene="yggP"
                     /locus_tag="ACHJ12_000329"
     CDS             complement(85287..86564)
                     /gene="yggP"
                     /locus_tag="ACHJ12_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4988176.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(86579..87967)
                     /gene="cmtA"
                     /locus_tag="ACHJ12_000330"
     CDS             complement(86579..87967)
                     /gene="cmtA"
                     /locus_tag="ACHJ12_000330"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4988177.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(87995..88438)
                     /gene="cmtB"
                     /locus_tag="ACHJ12_000331"
     CDS             complement(87995..88438)
                     /gene="cmtB"
                     /locus_tag="ACHJ12_000331"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4988178.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQDVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(88752..90743)
                     /gene="tkt"
                     /locus_tag="ACHJ12_000332"
     CDS             complement(88752..90743)
                     /gene="tkt"
                     /locus_tag="ACHJ12_000332"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4988179.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            91021..91779
                     /gene="loiP"
                     /locus_tag="ACHJ12_000333"
                     /gene_synonym="yggG"
     CDS             91021..91779
                     /gene="loiP"
                     /locus_tag="ACHJ12_000333"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4988180.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(91835..92578)
                     /locus_tag="ACHJ12_000334"
     CDS             complement(91835..92578)
                     /locus_tag="ACHJ12_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4988181.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFTMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(92565..93674)
                     /gene="dgaE"
                     /locus_tag="ACHJ12_000335"
     CDS             complement(92565..93674)
                     /gene="dgaE"
                     /locus_tag="ACHJ12_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO4988182.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGYIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(93678..94538)
                     /locus_tag="ACHJ12_000336"
     CDS             complement(93678..94538)
                     /locus_tag="ACHJ12_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4988183.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(94535..95284)
                     /locus_tag="ACHJ12_000337"
     CDS             complement(94535..95284)
                     /locus_tag="ACHJ12_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO4988184.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(95310..95795)
                     /locus_tag="ACHJ12_000338"
     CDS             complement(95310..95795)
                     /locus_tag="ACHJ12_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4988185.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(95806..96234)
                     /locus_tag="ACHJ12_000339"
     CDS             complement(95806..96234)
                     /locus_tag="ACHJ12_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4988186.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(96353..99151)
                     /gene="dagR"
                     /locus_tag="ACHJ12_000340"
     CDS             complement(96353..99151)
                     /gene="dagR"
                     /locus_tag="ACHJ12_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO4988187.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(99410..100330)
                     /gene="speB"
                     /locus_tag="ACHJ12_000341"
     CDS             complement(99410..100330)
                     /gene="speB"
                     /locus_tag="ACHJ12_000341"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4988188.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(100466..101197)
                     /locus_tag="ACHJ12_000342"
     CDS             complement(100466..101197)
                     /locus_tag="ACHJ12_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988189.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(101343..103319)
                     /gene="speA"
                     /locus_tag="ACHJ12_000343"
     CDS             complement(101343..103319)
                     /gene="speA"
                     /locus_tag="ACHJ12_000343"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4988190.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(103328..103459)
                     /gene="yqgB"
                     /locus_tag="ACHJ12_000344"
     CDS             complement(103328..103459)
                     /gene="yqgB"
                     /locus_tag="ACHJ12_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4988191.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(103553..103696)
                     /gene="yqgG"
                     /locus_tag="ACHJ12_000345"
     CDS             complement(103553..103696)
                     /gene="yqgG"
                     /locus_tag="ACHJ12_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4988192.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(103807..104058)
                     /gene="yqgD"
                     /locus_tag="ACHJ12_000346"
     CDS             complement(103807..104058)
                     /gene="yqgD"
                     /locus_tag="ACHJ12_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4988193.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            104114..105268
                     /gene="metK"
                     /locus_tag="ACHJ12_000347"
     CDS             104114..105268
                     /gene="metK"
                     /locus_tag="ACHJ12_000347"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4988194.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            105704..107098
                     /gene="galP"
                     /locus_tag="ACHJ12_000348"
     CDS             105704..107098
                     /gene="galP"
                     /locus_tag="ACHJ12_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4988195.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            107175..107672
                     /gene="yggI"
                     /locus_tag="ACHJ12_000349"
     CDS             107175..107672
                     /gene="yggI"
                     /locus_tag="ACHJ12_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4988196.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            107767..108474
                     /gene="endA"
                     /locus_tag="ACHJ12_000350"
     CDS             107767..108474
                     /gene="endA"
                     /locus_tag="ACHJ12_000350"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4988197.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            108554..109285
                     /gene="rsmE"
                     /locus_tag="ACHJ12_000351"
     CDS             108554..109285
                     /gene="rsmE"
                     /locus_tag="ACHJ12_000351"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4988198.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            109298..110248
                     /gene="gshB"
                     /locus_tag="ACHJ12_000352"
     CDS             109298..110248
                     /gene="gshB"
                     /locus_tag="ACHJ12_000352"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4988199.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            110357..110920
                     /gene="yqgE"
                     /locus_tag="ACHJ12_000353"
     CDS             110357..110920
                     /gene="yqgE"
                     /locus_tag="ACHJ12_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4988200.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            110920..111336
                     /gene="ruvX"
                     /locus_tag="ACHJ12_000354"
     CDS             110920..111336
                     /gene="ruvX"
                     /locus_tag="ACHJ12_000354"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4988201.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(111512..112492)
                     /gene="yggR"
                     /locus_tag="ACHJ12_000355"
     CDS             complement(111512..112492)
                     /gene="yggR"
                     /locus_tag="ACHJ12_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4988202.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            112510..113214
                     /gene="yggS"
                     /locus_tag="ACHJ12_000356"
     CDS             112510..113214
                     /gene="yggS"
                     /locus_tag="ACHJ12_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4988203.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            113232..113798
                     /gene="yggT"
                     /locus_tag="ACHJ12_000357"
     CDS             113232..113798
                     /gene="yggT"
                     /locus_tag="ACHJ12_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4988204.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            113795..114085
                     /gene="yggU"
                     /locus_tag="ACHJ12_000358"
     CDS             113795..114085
                     /gene="yggU"
                     /locus_tag="ACHJ12_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4988205.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            114093..114686
                     /gene="rdgB"
                     /locus_tag="ACHJ12_000359"
     CDS             114093..114686
                     /gene="rdgB"
                     /locus_tag="ACHJ12_000359"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4988206.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            114679..115815
                     /gene="hemW"
                     /locus_tag="ACHJ12_000360"
     CDS             114679..115815
                     /gene="hemW"
                     /locus_tag="ACHJ12_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4988207.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT        28166 a        30815 c        28920 g        27923 t
ORIGIN      
        1 tcatcataat ttccggtgaa atttgtcttt gccggaagat tgatcaccag gctgttgccg
       61 cgttccgcat catccttcag tccagccatg cgcggtgtcc agtcctccgg ctgctggtca
      121 acaaagccgc tgtagcaacg tgcctccttt acacccgcca ccgcaagcag tgttgctatc
      181 tgtaaaagtg ctgtctctga tatttcacca gcacgataaa cgcgcacgta attgcgccct
      241 ttgtcgatga tttttgtgtc ttccaggctc gccagttgtt tcggtccaag aataacgggc
      301 ggtgtatcat ctgctgcaat atgctttccg gcccattcgc tccactcttt cgcatgtgcc
      361 catgcatcac tacctgcaaa gataatgact tcggtcattt tgtctttcgg ctggagcttt
      421 aaattcggtg cttttttcat tttttgctcc tgaatacgtt aatcatgttt ttcatctttc
      481 ggatgttcgc ctctgctttt gtattgctgg cttaccacgt ggcacggcct gcatcaggga
      541 aaaatcacga ctgaactgat acaggggcat cacgcagtca tagtcatacc cttcacggcg
      601 gtaggtgatg cgtcgttctc tcgtgctgat aatcgtcacg atgccgccgt acttatcgcg
      661 ataaatatcc ccgcacataa attcggggtg agttttgcta ctggcagtta agccagatat
      721 tttcgatttc atgtaattag cctcctgaag gagtcgtctt gatatttatt tcccgtttat
      781 ttttagccgt tattgttcgc cgcgtttttc ttcctccgtc attaaccaca ccgccacatt
      841 gctggaaagt tgtttgataa gtcccgcaat aacagaaagg tcatggtcgt ctatttcttc
      901 gtggttgcgt tcaatcatgc gttcaatcat gcggctgatt acttcgatct gttgcgcttt
      961 cagtgaggct tctgccagtg tcatttcctg cattactgca cccccttaat ttcatgaacg
     1021 gcgtggataa ttacgccgga taaaccggct ttagctgatt tgttaattac tgtgctgatt
     1081 gcagcctgga cgttttcggc cttcacgttc tgcgtgatgc caacggtata gccacgcttg
     1141 ttaacagcac gggaaaagac gcggaaggtt ttaagcatga gtcacctcct gacggatacg
     1201 ggcgacaaaa ataagatgtg cgtgcggcag ctgggcgcga gcttcgtgct cagtggcagc
     1261 ggtaacggtg aatattgcaa tgctgtctgt caggcactgc ataaagcgcc aggtgaattt
     1321 attcaggttg tgcgagtccc gcccttgcgg gtgtgtgata tgatttctca tagctgcctc
     1381 gatactcttg ctatcgttgg tggtcagagg ctgcgaaagg acggcaatcc ttttcagcct
     1441 cgtttgcatt gagtgataaa cactcaactg gattccagta taatcactaa gtggaatcca
     1501 cttcaagcgt tttgttttgg tctttttcgt gtatactgga ttccagtaat cacaaaggga
     1561 agcagaaatg gaaagagacc atatcaataa caaatcacag aaattacagg ctcgcgcccc
     1621 gcatgaagtt gttgaggcta tggagcaagt aaaagaaaca ggcgagagca ctgcgcaatt
     1681 tatcgttaca gccatgcgag gcgagatcaa acgccgccag cgccgcaagg ccaaagaatc
     1741 agaataatca ctatcagcgc cgtggtgtga ggtactacgg cgcattgctt tacagggcag
     1801 cagcatgaac aaaaagtacc aatccattac agatattttt gccgaacaac gaagacagct
     1861 tttgcaccgc atacttgagc gccaactgga actgaaacgc gctaaagaga tatcagaacc
     1921 cgaagagtta acgcctcttc ccggagtgaa aaaacaccca caaaaacgat ttgcccttga
     1981 agattttgtc gagaaatacc cacggcggtt aaaagccact aaaaaccgac cgcctggctg
     2041 atagccttgt ccaccagcta cactcatggc atagttggtg atgcgcttga tttcctttat
     2101 ctccactggc gaccctttcg tggtcgcctt tgttttgcct gccattcctg catcatgcaa
     2161 atattcgcgc cgtacagagc caccagcgcg ataatttgat aaaacctcct ctaagtattg
     2221 cttaacgtgt ttcatacgct cagcggcgaa atggctcacg atatggcgca tgtaatcaaa
     2281 ctgatcgcta ttcagcagag caaacttatg ttcacgctct ccgtgttgat tggtttttaa
     2341 tcgctccgtt tgaaacggaa ggatacccag ttcacgcaat ccggttttgt gcttctgaat
     2401 aagcgaataa aggcttttgt gctttatgcc gatgcgttca gcaaacaggc gggaatcaat
     2461 acgcgactct gctttgtggc gaatcaatat atcgttctgg ctgatttcag gctgaacgaa
     2521 gccctgcccc tgtacgggca cattcggacg tttcattgaa aactcctgct atcgaatcag
     2581 gtttagtgct gggggagaat atccccctcc caaaagatta ttacgcggcg ttgttgcgag
     2641 aagcatcaat acgctggtta atccattcgt cgacttcact ttcaataaaa gccacggagc
     2701 gggaaccaat ttttaccggt ttagggaaac gccccaggct gatcaggcga taaatccatg
     2761 cgcgaccgta accagtacgg cgcataacct cagaaagacg gatgaatgaa ttgttattag
     2821 ccatttatca atctccttaa tgttgacgcg aaaatcccct cctgtggctt agcaagagtt
     2881 ttatttgcgt caaattacga acacgacaaa aaccacaaca tgaaaacatg ttgtggtatt
     2941 gcataagata aattcagatt gtttggttgg tggttggtat gctccagcaa ggtagagcta
     3001 ttcccgcttt tcggcggggt cacacgatta ggcgtgacgt ccagtagttt taaagagcgc
     3061 cctttttcag ggcatggtgg gcaggcaaga gagcaattgc gctttgtcag cgctccgcag
     3121 cctcattggt gaatcattac atgcattgta aaggtgcatg tatccctatt ctttcctgca
     3181 tcaggaaagc accaaaggct gttaaagagc atccagcttt tcagctgcgt agaagactcc
     3241 atcttcgttg ttacaaggag cgccgtccat acatgatggg gtaactgcgt gaaaaaccat
     3301 ctgtaaggat attacctgta gctttccgtg tacgttagta tacatctgta tacttgtcca
     3361 gtcgttttta tgcacataca ccaaacttgc cgtgcaccac gttaccgccc tgttccagtg
     3421 agtccatata gtcggcgtac cactggagca tctcccgccg tccgtccagg tattgcgcgt
     3481 gattgtatgt gccacggatc gagtttttat cgacgtgcgc cagctgcgtt tctatccacg
     3541 cggagttata accctgctca tgcaaaatcg tactcatggt atgccggaac ccatgaccag
     3601 taacgcgccc tgcatagcca atacgacgaa taagcacgtt catagccatt tcactcatcg
     3661 gcttactgtg ctgtattcga ccagggaaaa taaacggata ctgccccgtc atgtgtttta
     3721 actgttccag aatagcgatc gcctgttctg aaagtggcac gcaatgagga cggcgcattt
     3781 tcattcgttc tttcggcact tcccacagac gattatcaaa atcaatctcc tgccactctg
     3841 cctgccgtaa ttcacctggg cgaagtcctg taagaatcaa caatcgcata cccagcttga
     3901 cgatctggct tccggtgtac gtattaaaaa cgcggaaaaa ttccggtagc tcatcaactt
     3961 tcagatacgc gtaatgctct tttttgtgtg gtgcaaatgc gcttgccaga tcaggagcgg
     4021 ggttatattc agcgcgtccg gtaattattg cgtaccggaa cacttcgcca catcgctggc
     4081 gaaccttacg caatttttca gttgcgccac ggtcattaag acgggataga accgcgagca
     4141 attccatcgg cttaatctct gcgatgggcc tacctccaat gtacggaaaa acgtcttttt
     4201 caaacgtctc tatcatttcc tctgcatatg agggagacca tcggtccagg cgtttttcat
     4261 accactcacg ggcgatcgcc tcaaaggtgt tgccgcattt gttcgcctgt tctcgctttt
     4321 gggcctttct gacctcactc ggattaactc caccagcgat aagccttttg gcttcatccc
     4381 ttgccgtcct agcatcagaa agggacacct caggataaac acccagcgac aacattttgg
     4441 gcttgcctgc aaacctgtat cgcaatcgcc agcccttcga accgttcggc tcaatgagta
     4501 acgaaagccc gttgccgtca ttgagtgtat aggctttctc ttttggcttc gcgcgacgga
     4561 tagcaaggtc tgaaagcaac atgtgtatag cccccaaaaa acgattttgt cctatacgca
     4621 ttactataca cttctgtgtg tagatttagg tagatgactg tagacggttg tagacgagga
     4681 aaatcgctgg aggccttgat attcggggga ttggtagact ttagtagacg ttggtagatg
     4741 tttgtttgga gcgggcgaag ggaatcgaac cctcgtatag agcttgggaa gctctcgttc
     4801 taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt ataaacctta cgctctcctt
     4861 agcaagtgcc gcgctgctga ctgctgatta attcgccatc agcattttgg cttgctgccc
     4921 tgcggcggca agtaacgtag cggatcaatt gccgttgcac ggtaacgaat ctggaaatgc
     4981 aggcgaacag atgccgcatc cgtgctcccc atagtggcga ttttttgccc agccttcacg
     5041 ctttgcccat tatttaccag catcgtgtca ttatgggcgt aagccgtaat gtaatcttca
     5101 ctgtgtttaa tcatgatgag attaccgtag ccacgcagct ggttgcccac atacaccacc
     5161 tttcctgcac ccgcggcgta aataggtgta ccccgtggag ctgagatatc aatcccttta
     5221 ttgccgccat ctgctgtcga atacggcatg ataactttcc ctgtcgttgg ccataaccaa
     5281 caacgttgcc ctactggcgg ccaggaagat ttcggtaccg ctgatgacgg tgtaaccgat
     5341 gcggttttgg tcgttgattt ggcggttgat ttacgtgtac tactgctact tttcgcccca
     5401 cccagtttta gtttctgacc aacttcaatg gtgtaagggg gggaaatgcc gttcagtcgc
     5461 gccagttctt ttacgctggt tcccgtggtg cgcgaaatac gatatagcgt atccccccgt
     5521 ttcacggtgt aaacggagcc ggaatacgtt cctgtatcgg atgatttgct acccgaacag
     5581 cccgccaaaa gcagtccaac cgataacaac atcacgatac ccagagattt tttattcagg
     5641 cgtcccgcac tcaaaatctt tcctcgctta aaaaacaaga cgccttatca tagcagccag
     5701 cctcttgata cccaatctct tgcttctgga aaaacgtggt aatagagaaa gaggttaatt
     5761 tcatttggca aagggaagta tcatcagcaa aatattcgct gtagaggtat cgaacttcct
     5821 gactttttac cctatttccc atcctgatgc gtaataatcc caatttatca cattgatttt
     5881 atcgccactc atattgatac gtatcactgc ccctattcag agtcctgttt cgtatcatat
     5941 ctgtgttttt ttaacgtcat cacacttcct acctccccta acgcttatcg tcgtttctgg
     6001 cgtaaatctt gcctgcttag actaaatctt tgccataaga accaaatgtt caaatcgagg
     6061 ggatttctat ggaagcgcgg gaagcaaccg ctacgggtga atcatgcatg cgcgtcgatg
     6121 ccattgctaa ggtcaccggg cgggcacgat atactgacga ttatgttatg gcgggcatgt
     6181 gttacgcgaa atatgtacgt agccctatcg cacatggtta tgccgtaagt attaatgatg
     6241 aacaagccag aagtttaccg ggcgtactgg cgatttttac ctgggaagat gtgcctgata
     6301 ttccattcgc tacagctggg catgcctgga cacttgacga aaacaagcgc gataccgccg
     6361 atcgcgcact gctaactcgc catgttcgtc atcatggtga cgccgttgcc atcgtcgtgg
     6421 cccgcgatga actcacggca gaaaaagcgg cgcaattggt cagcattgag tggcaagaat
     6481 tacccgttat caccacgcca gaagcggcgc tggcagaaga cgctgcacca atccataacg
     6541 gcggcaattt actgaaacaa agcacgatgt cgacgggcaa tgtccaacaa acaatcgatg
     6601 ccgccgacta ccaggtacag gggcactatc agacccccgt tattcaacat tgtcacatgg
     6661 aaagcgtaac atcgctggcg tggatggagg atgactcgcg aattaccatc gtttccagca
     6721 cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc gctggatatt ccctggtcat
     6781 gcgtacgagt catcaaacca tttgtcggtg gcggttttgg taataaacag gatgtactgg
     6841 aagagccaat ggcggcattc ctgaccagca agcttggcgg cattccggtg aaagtttccc
     6901 ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca cgcttttacc attgacgggc
     6961 aaatgggcgt gaaccgcgac ggaacattga aaggttatag tctggatgtt ctgtctaaca
     7021 ccggcgctta tgcatctcac gggcactcca tcgcttctgc gggggggaat aaagtcgctt
     7081 acctttatcc tcgttgtgcc tacgcttaca gttcaaagac ctgctatacc aacctcccct
     7141 cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt atttgccgtt gagtctatgc
     7201 ttgatgacgc cgcgacagcg ttaggtattg atcctgttga aattcgttta cgcaacgccg
     7261 cccgcgaagg agatgctaat ccgctcacgg gcaaacgtat ttacagcgca gggttgccgg
     7321 agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa acgccgtgca gaatgccaga
     7381 accagcaagg caatttgcgc cgcggcgttg gcgtcgcctg ttttagctac acctctaaca
     7441 cctggcctgt cggcgtagaa atagcaggcg cgcgccttct gatgaatcag gatggaacca
     7501 tcaacgtgca aagcggcgcg acggaaatcg gtcagggtgc cgacaccgtc ttctcgcaaa
     7561 tggtggcaga aaccgtgggg gttccggtca gcgacgttcg cgttatttca actcaagata
     7621 ccgacgttac gccgttcgat cccggcgcat ttgcctcacg ccagagctat gttgccgcgc
     7681 ctgcgctgcg cagtgcggca ctattattaa aagagaaaat catcgctcac gccgcagtca
     7741 tgctacatca gtcagcgatg aatctgaccc tgataaaagg ccatatcgtg ctggttgaac
     7801 gaccggaaga gccgttaatg tcgttaaaag atttggcgat ggacgctttc taccaccctg
     7861 aacgcggcgg gcagctctct gctgaaagct ccatcaaaac caccactaac ccaccggcgt
     7921 ttggctgtac ctttgttgat ctgacggtcg atattgcgct gtgcaaagtc accatcaacc
     7981 gcatcctcaa cgttcatgat tcagggcata ttcttaatcc actgctggca gaaggtcagg
     8041 tacacggcgg aatgggaatg ggcattggct gggcgctatt tgaagagatg atcatcgatg
     8101 ctaaaagcgg cgtggaccgt aaccccaatc tgctggatta caaaatgccg accatgccgg
     8161 atctgccaca actggaaagc gcgttcgtcg aaatcaatga gccgcaatcc gcatacggac
     8221 ataagtcact gggtgagcca ccaataattc ctgttgccgc tgctattcgt aacgcggtga
     8281 agatggctac cggtgttgca atcaatacac tgccgctgac gccaaaacgg ttatatgaag
     8341 agttccatct ggcaggattg atttgaggat aacatcatgt ttgattttgc ttcttaccat
     8401 cgcgcagcaa cccttgccga tgccatcaac ctgctggctg acaacccgca ggccaaactg
     8461 ctcgccggtg gcactgacgt actgattcag ctccaccatc acaatgaccg ttatcgccat
     8521 attgttgata ttcataatct ggcggagctg cggggaatta cgctggcgga agatggctcg
     8581 ctacgtatcg gctctgcaac gacatttacc cagctaatag aagatcctat aactcaacgt
     8641 catctcccgg cgttatgtgc tgcggccacg tccattgctg gaccgcagat ccgtaacgtc
     8701 gctacctacg gtggaaatat ttgcaacggt gccaccagcg cagattctgc cacgccaacg
     8761 ctaatttatg acgcgaaact ggagatccac tccccgcgcg gtgttcgttt cgtcccgatt
     8821 aatggctttc acaccgggcc gggcaaagtg tctcttgagc atgacgaaat cctcgtcgcc
     8881 tttcattttc cgccacagcc gaaagaacac gcgggcagcg cgcattttaa atatgccatg
     8941 cgcgacgcaa tggatatttc aacgattggc tgcgccgcac attgccgact ggataacggc
     9001 aatttcagcg aattacgcct ggcatttggt gttgccgcgc caacgccgat tcgctgccaa
     9061 catgccgaac agactgcaca aaatgcgcca ttaaacctgc aaacgctgga agctatcagc
     9121 gaatctgtcc tgcaagatgt cgccccgcgt tcttcatggc gggccagtaa agagtttcgt
     9181 ctgcatctca tccagacgat gaccaaaaaa gtgattagcg aagccgtcgc cgcggcgggg
     9241 ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga atgcaccatt aacgggatgc
     9301 cttttcagct tcacgccgca ccaggcacgc cgctctcgga attactccgc gaacaaggac
     9361 tgctaagtgt caaacaaggg tgctgcgtgg gtgaatgtgg tgcctgtacg gtgttggtcg
     9421 acggcacagc aatagacagt tgcttatacc ttgccgcctg ggctgaagga aaagagatcc
     9481 gcacgctgga aggtgaagcg aaaggcggaa aactttctca tgttcagcag gcttatgcga
     9541 aatccggcgc agtgcagtgc gggttttgta cgcctggcct gattatggct accacggcaa
     9601 tgctggcgaa accacgcgag aagccattaa ccattacgga gattcgtcgc ggactggcgg
     9661 gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa tacagttctg gattgcgaga
     9721 aaacgaagta aaaggatatc cggcctgaat tcaggccgga ttcactgagg ttatgtgttt
     9781 aacaactcat atttcttaat cttgcgatag agcgtagcaa tgccgatgcc cagttcatca
     9841 gcaacttgct tcttgctgtt atgacgtgaa agcgcctcgc ggatcatttg cttttccatc
     9901 tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt gcgcctcact gacctctgtt
     9961 acatcacttt gctccgttgt gccattattc agcagatttg gcggcaatag cgtgctgtcg
    10021 ataacttcac ctgaaggaac tacgttaacc agatattcca tcaaattgct taactcgcgc
    10081 aggtttccgg gccaacgatg cttacgcaat atttcgacga catcgggagc aatgccagga
    10141 taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt aatgcaccaa tagttcaata
    10201 tcttcctgac gttcacgcag cggtggcaga gttatcggga taacattaag tcggtagaag
    10261 agatcttcgc ggaatttacc ttcggcaatg aactgggcca aattctgatt agttgcagaa
    10321 atgatgcgaa tgtcgacttg tattgggcta ctggcaccaa tcggcagaat ttcacgcgcc
    10381 tcaatagcgc gcagtaattt agcctgcaac attaatggca tatcacctat ttcatcgaga
    10441 aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt taccgttggc agaagcgcca
    10501 gtaaatgcgc ctttaacata accgaacagt tcgctttcca gaagctgctc cggaatcgcg
    10561 gcacagttga tagcaataaa gggtttattc cgtcttccgc tcaacttatg gattgctcgg
    10621 gcgacgactt ctttacccgt gccgctttca ccaaccacca taacgctgga tgggctgggt
    10681 gcaatacggc taatgagtcg ttttaattgc cgcataacac ggcactcgcc aaccaattgt
    10741 tcaatatgtg gttcatcagg tgcatttgct acagaaaaac tggtatgcga ttggtgaaat
    10801 gccattaaaa ataattgtcg gccctgaatg ttatgcaatt gaccaatgat taattcactt
    10861 ttatcgtccc acgaaacaat atgctgcata tgtccatgag taaaattact ctcaaatgtt
    10921 aatggtctga aacggatagg tttcccaata atattatttt gtacaacacc aagtgttttt
    10981 aaggcagtct gattaacaaa ctgaacccga ctttcatcat caacaactaa tacgccctga
    11041 tccatattat cgatcatggt cgcgaatatt ttactgatgt tatctcctgg cccctgatcc
    11101 tccagaagtt tcgaaacaaa aatggtggat atatggcgaa cataatcaga aaattcgcgt
    11161 aaattatcac tgatatgctc ttgttgctcg tgggtaacgg caatcaaact tatcacccca
    11221 acacaacgat cctgtaaaat gacaggcgta cccagaaatg ctttttcacg gcaattttct
    11281 ttactatcgc aaccttcgca aaggggatcg aagcgagact gtgtcacaac tttttcagtt
    11341 ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc tcaactggcg accaagaaac
    11401 ttcccatacg cgcccgttcc ggcgacgcga cacaagtttt catcaacgat ctcaacctca
    11461 agctgcaaaa cgctggcaag cattctggca aaacgctgaa ttgtcggttg aatttgcatc
    11521 aatactgact gcgtagtagc aagctccata gctttacctt ccagacttac ttaaaagtcg
    11581 atcattgaag acgttgatgg ttcacagatc atgatgatat taactcaggc gaaattggct
    11641 ttgataaaaa cataagattt ttatcatttt ctaatgaaat tatggaagag atatcacatt
    11701 tctatatcaa tatgagaatt acggcggtga gtttatcaaa ctgaagagag atagcctgtc
    11761 cctttatctt atttctgata cttagcagca aataaataac gagataaaaa aagccaaacg
    11821 ttttcgtatt ttacaaacaa ccggaagctg gcatcaattt gtgatcaacc ccacacatta
    11881 tccgtcaaat tagtcttttg cagccccgtg gataattctg gcacacttat tgttagtccc
    11941 aggtatagct gtgaaaacac caatcacttt ggcaagtcac agtgaaataa accactttgc
    12001 ctgtcattcc actaccggga ctttatgatg aaaactgtta atgagctgat taaggatatc
    12061 aattcgctga cctctcacct tcacgagaaa gattttttgt taacgtggga acagacgcca
    12121 gatgaactga aacaagtact ggacgttgcc gcagcattaa aagcactgcg tgctgaaaac
    12181 atctcaacca aagtctttaa tagtggatta ggtatttccg tattccgcga caactccacc
    12241 cgtacccgct tctcttatgc ttccgcgctt aacctgctcg gccttgcaca acaagatctc
    12301 gacgaaggca aatcacaaat cgctcacggc gaaaccgtgc gtgaaaccgc caatatgatc
    12361 tccttctgcg ccgacgctat tggtattcgc gacgatatgt atctgggcgc aggcaacgcc
    12421 tatatgcgtg aagttggcgc tgcacttgat gacggttaca agcagggtgt actgccacag
    12481 cgtccggctt tagtgaacct gcaatgcgat attgaccacc cgactcagtc aatggctgac
    12541 ctcgcgtggt tacgtgaaca ctttggttca ctggaaaacc tgaaaggtaa aaaaatcgcc
    12601 atgacctggg cctactctcc aagctatggc aaaccgctct ctgtaccaca aggcatcatc
    12661 ggtctgatga ctcgcttcgg tatggatgtc accctggccc atccggaagg ctacgacctg
    12721 atcccggatg tagtagaagt cgcgaaaaac aatgctaaag catctggcgg tagcttccgt
    12781 caggtcacca gcatggaaga agcattcaaa gatgcagaca tcgtttatcc gaagtcatgg
    12841 gcaccttaca aagtgatgga acagcgtacc gaattgctgc gcgctaacga tcacgaaggc
    12901 ttaaaagcac tggaaaaaca gtgtctggca cagaacgcgc aacacaaaga ctggcattgt
    12961 actgaagaga tgatggaact gacccgtgat ggcgaagccc tgtacatgca ctgcctgcca
    13021 gctgatatca gcggcgtatc ctgcaaagaa ggggaagtga ctgaaggcgt attcgaaaaa
    13081 taccgtatcg ctacctacaa agaagccagc tggaagcctt atatcatcgc cgcgatgatc
    13141 ctgtcccgta aatacgccaa accaggtgca ctgctcgagc aactgctgaa agaagcgcaa
    13201 gaacgcgtga aataacccct cgggccgatc agctggtcgg ccctctattt ccagaggcca
    13261 aaaggatagg atatgtccgt tttctcattg aagattgata tcgccgataa caaatttttc
    13321 aacggcgaaa catcaccgct cttttcgcaa agccaggcca aactggcgcg ccagttccac
    13381 cagaaaatag ctggttatcg cccaacaccg ctttgtgcgc tggacgatct cgcaaacctt
    13441 tttggtgtga agaaaattct cgtcaaagac gaatcaaaac gattcggtct gaacgccttc
    13501 aaaatgcttg gcggtgcgta cgccatcgct caattattgt gtgaaaaata tcatcttgat
    13561 atcgaaacgc tgtcatttga gcacctgaaa aatgccatcg gcgaaaaaat gactttcgcg
    13621 accaccaccg acggcaacca cgggcgcggt gtggcgtggg cagcacagca actcggacag
    13681 aatgcggtga tttacatgcc gaaaggttct gctcaggaac gcgttgacgc cattctgaac
    13741 ctcggtgccg agtgcatcgt cacggatatg aactatgacg ataccgttcg cctgaccatg
    13801 caacacgcgc agcagcacgg ctgggaagtg gtacaggaca cggcatggga aggttacacc
    13861 aaaatcccaa cctggatcat gcaaggctac gcaaccctgg cagatgaagc cgtcgagcaa
    13921 atgcgtgaaa tgggcgtaac cccgacgcac gttctgctgc aagccggtgt cggagcaatg
    13981 gccggtggtg tgctgggtta tctggtcgac gtctacagcc cgcaaaatct gcacagcatt
    14041 attgttgaac ctgacaaagc tgactgtatt tatcgctccg gcgtcaaagg cgacatcgtc
    14101 aacgttggcg gtgatatggc caccatcatg gcgggcctgg cctgtggcga acctaacccg
    14161 ctgggctggg aaatcctacg taactgcgcc acccaattca tctcctgcca ggacagcgtt
    14221 gccgcattag gtatgcgcgt gctgggtaat ccgtacggca acgacccgcg catcatctcc
    14281 ggtgaatccg gcgctgtcgg tttgggcgtt ctcgcggcgg ttcattatca cccgcaacgt
    14341 caaagcctga tggaaaaact ggcgctgaac aaagatgccg tggtgctggt tatcagcact
    14401 gaaggcgaca ccgacgtgaa gcactaccgc gaagttgtct gggaaggcaa acacgctgta
    14461 gcaccttaat caccctattg gaactactcc ctgcaaaacg gggagtaaaa aatctggaga
    14521 aaaataatgg ctaagaatat tccattcaaa ctgattcttg aaaaagcaaa agattaccag
    14581 gcggacatga ctcgcttcct gcgcgacatg gttgctattc ccagtgaaag ctgcgacgaa
    14641 aaacgcgtag tacatcgtat taaagaagag atggaaaaag tcggcttcga taaagttgaa
    14701 atcgacccga tgggcaacgt tctcggttat atcggccacg gcccgcgtct ggtggcaatg
    14761 gacgctcata tcgataccgt cggcattggc aacatcaaaa actgggactt cgatccgtac
    14821 gaaggcatgg aaactgatga gctaatcggt ggtcgcggta cttccgacca ggaaggcggc
    14881 atggcatcta tggtttatgc cggtaaaatc attaaagacc tcggtctgga agatgaatat
    14941 accctgctgg ttaccggtac tgtgcaggaa gaagactgcg acggtctgtg ctggcagtac
    15001 attattgaac aatccggcat tcgcccggaa tttgtggtca gtaccgaacc aaccgactgc
    15061 cagatatacc gtggtcagcg cggtcgtatg gaaattcgta ttgatgttca gggtgttagc
    15121 tgccacggtt ctgcgccaga acgcggtgac aacgccattt tcaaaatggg tccgattctt
    15181 ggcgaattac aagaactctc ccaacgtctg ggttatgacg aattcctcgg caaaggcacc
    15241 ctcaccgttt ctgaaatctt cttcacatcc ccaagccgtt gcgctgtagc agatagctgc
    15301 gccgtctcta ttgaccgccg tctgacctgg ggcgaaacct gggaaggcgc gctggacgaa
    15361 atccgcgccc tacctgcagt acagaaagct aacgcggttg tttctatgta caactacgac
    15421 cgtccgtcct ggactggcct ggtttaccca accgaatgct acttcccgac ctggaaagtg
    15481 gaagaagatc acttcaccgt taaagcactg gtgaatgcct acgaagggct gtttggcaaa
    15541 gcgccggttg ttgataagtg gaccttctca actaacggcg tatctatcat gggccgtcac
    15601 ggcattccgg tgatcggctt tggcccgggt aaagaacctg aagcgcatgc acctaacgaa
    15661 aaaacctgga aatctcacct ggtgacctgt gccgcgatgt acgctgcaat cccgttaagc
    15721 tggctggcaa cagagtaatt actcttcact tattcccctc cggttcgccg gagggttttt
    15781 ggagtttgct atgcgcgtat tgatcaaaaa cggcactgtc gttaacgcag atggacaagc
    15841 caaacaggat ttgctgattg aaagcgggat tgttcgccag ttgggcaaca atatttcgcc
    15901 gcagctcccg tatgaagaaa ttgatgccac tggctgttac gttttccctg gcggcgtgga
    15961 tgtccatacg catttcaata ttgatgtcgg catcgcgcgc agttgtgatg atttttttac
    16021 cggtacccgc gcagctgcgt gtggcggtac aacaaccatt attgaccata tgggatttgg
    16081 cccaaacggc tgtcggttac gccatcaact ggaggtttat cgtggttatg ccgcccataa
    16141 agcggtcatc gattacagct ttcacggtgt gatccagcac attaatcacg caatcctcga
    16201 cgaaatcccg atgatggtcg aggaaggact gagcagtttt aaactctatt taacctatca
    16261 atacaaactc aacgatgacg aggttttgca ggcattacgc cgtctgcatg aatccggcgc
    16321 gctgaccacc gtgcacccgg aaaatgatgc ggctatcgcc agcaagcggg cggaatttat
    16381 cgccgcaggg ttaaccgcgc cgcgctatca tgccttgagt cgccctctgg aatgcgaagc
    16441 ggaagccatc gcccgcatga ttaacctggc acaaattgcc ggtaacgccc cgctctatat
    16501 cgtgcacctg tctaacggct taggtctgga ttatctgcgt cttgcccgtg cgaatcacca
    16561 gccagtctgg gttgaaacct gcccacaata tctcctgttg gacgaacgca gttacgatac
    16621 agaagacggc atgaagttca ttcttagccc accgctgcgt aacgtacgcg agcaggacaa
    16681 actgtggtgt ggcatcagcg atggtgcgat tgacgtggtg gcgaccgatc actgcacctt
    16741 ctcgatggct caacgcctgc aaatttctaa aggcgatttc agtcgctgcc caaatggctt
    16801 acccggtgtg gaaaaccgca tgcagttact gttttccagt ggcgtgatga cgggacgtat
    16861 aacaccggaa cgctttgttg aattaaccag cgcaatgccc gccaggctgt ttggcctgtg
    16921 gccgcaaaaa ggattattag cgcccggttc cgatggcgac gtggtgatta tcgacccacg
    16981 tcagagccaa caaattcagc atcgccatct ccacgacaac gccgactact cgccatggga
    17041 gggttttacc tgtcagggcg cgattgtcag aaccttatcc cgtggtgaaa cgattttctg
    17101 tgacggcacc tttacaggca aagccgggcg aggtcgtttc ctgcgacgca aaccgtttgt
    17161 ccctcccgtg ctctaaccac tgtcagggat ctatacacaa tcaagaatga tgagtgagga
    17221 aaaatgagta agaaaattgt tctcgccctg ggcgggaatg cgctgggcga tgacctggcc
    17281 ggacaaatga aagcggtaaa aattacttct caggcaattg ttgatttaat tgcccaagga
    17341 catgaagtta tcgtcactca tggtaacggg ccgcaggtgg gcatgattaa ccaggccttt
    17401 gaagctgctg caaaaactga agcgcactcg ccaatgctgc cgatgtctgt ctgcgttgcc
    17461 ttaagccagg gttatattgg ttacgatcta caaaacgcct taagagaaga actgctttct
    17521 cggggcatta ataaaccggt agcaacgctg gttactcagg tcgaagtcga cgctaacgat
    17581 ccggcattcc tcaacccgac caagccgatc ggttcgttct ttaccgagca agaagcagag
    17641 caactgacaa aacagggtta cactctgaaa gaagatgctg gtcgcggcta tcgccgtgtt
    17701 gttgcctcgc caaaaccggt tgatattatt gaaaaagaaa cggttaaagc tctggtagat
    17761 gcaggccagg tggtgatcac cgttggcggt ggtggtattc ctgttattcg tgaaggtaac
    17821 catctgcgtg gtgccagcgc ggttatcgat aaagactggg ccagcgcccg tttagcagaa
    17881 atgattgatg ccgatatgct gatcattctg actgcggtag aaaaagtggc cattaacttc
    17941 ggtaaagaga atgaacagtg gctcgatcgc ctgtcattaa gtgatgctga acgctttatc
    18001 gaagaaggtc attttgcgaa aggttctatg ctgcctaaag tggaagctgc cgcctcgttt
    18061 gctcgctctc gcgcaggtcg tgaagcactg attaccgtat tgagcaaagc gaaagagggg
    18121 attgaaggca aaaccggaac ggtgatttgt cagtaatttt aaggccgggt gggtaatacc
    18181 atccggccat tttcatttaa gccacttcca gcacttcttt tgtcgctttc acgccctgat
    18241 gcatcaacat cattaacgcc tcaagtacgc cgccgccaat cgcccgggct ttatcagaaa
    18301 cgctggtgaa atcagccgtt tcaccacgag gatcgatatc accaatttta aaaccaccga
    18361 ccactgccag gccgtcgttc aacaagccac gcaccatccc cgtcaacggt gctttaattt
    18421 catgctcacc aatccaggca atcacatcgc cctctttcac cagatcgcct aatttcacgt
    18481 tggatcgcat aatgcctgca gcaggagcac ggatcacccg tcgggtggta tgccccataa
    18541 tattgccagg aacaccggta ttctcctgcg cacaaccaga gtaaatcacc tgaccgagcc
    18601 agtgcccgcg atttgtttca attaccgcat gacaatcctt ccctgcagta aagcccggcc
    18661 caagcgcgat tgttactggt gccatatctg cccgcgttcc caaattctgt ttcgccagaa
    18721 tagcgtccac cacgcaaagc ggtttcagtt catcaagcaa tgaacaggcg ggatctgcca
    18781 tcacagggat gaatccgcgt tcggtaagtt tcatcgcttc cgcagagctg gttgccaggc
    18841 gagcagtaac gccttcgacc gtcatttcgc catcgaacac ggcctgggca aacgccacgg
    18901 tacaacgaat cactgtcggt ttttccactt ccagcatgat cactttaaaa cccgcatgat
    18961 acagacgtag cgccacacca ctggcgatat ctcccgcacc gcgaatcacc accagctggt
    19021 gtccggaagg atgagaaggt ttcatcatca gcccacccgg cgcgttattt ttcacctgta
    19081 atatttctgc cagcacgctg atggcgatct cctgcggcgt ttccgcacct atgttgtaac
    19141 caacgggcgc atgtaaacgg gcaatatgtt cttcagccac gcctttctca cgcaattgac
    19201 gcaggaaaag ctgaaccttg cggcgacttg ccagcaaccc caaccatgca atgggctgtt
    19261 caatgagttt gtcgagggct tcacgatcct gattattcgt ggcaatcagg acaaaattat
    19321 cagggcgaat atccagtgct tccactgctg caccaaacga ctcagcatgg agaagcgtgg
    19381 ttgatggagg gaatagttca ggattgaggc tttcgcgata aatatcggca acggcaatat
    19441 caaatcctaa tagcgccgca ctctgggcta tcgcccggtt gacatgcccc gcgccaatca
    19501 acaccagacg tggacgcata ccatggacgc tgataaatac tgacattgca cctccacaat
    19561 ctgacccgac agcatccgca ccgttacgag ccatacgtcc atggaataat cgcggcttac
    19621 gttcctgcaa tgcctgaagt gactcttcaa tcaccttccg ctcaaccatt ccgccaccaa
    19681 ttgtaccgac gatagaacca tcggcgcgca ctaacatttg tgcagaatgg cggggagtcg
    19741 agcctcggct atcaacaatt tgcgccatcg caaacggaca attttgctct tcgagttttg
    19801 cagcctctgt gaaaatattc ataccaacct caatggatat cctttcagta acccggaata
    19861 cccgggccgc taattcacaa atctgcgcgc gattgcagga tgttcttgta tatcgcccag
    19921 ccagattgcc tctacgtcgt gttgttgcag cggttgaagg agctcgcttt gtgcaatcgc
    19981 attctcacat tgagaaaaac ggttaatgaa ccagactcgc cgacaacctt gcggtacgtt
    20041 tttaaacgcc ccctgagggt ggcgaaccag cgcaacgaga tcgctcagtt gcaaggttgc
    20101 atcaggtgtt aacccggtaa tatcagcaaa ctgcgaccag cgatggacat tttctgtgct
    20161 cactttcgcg cccaaagtat gtcctcccat cacagcaatc acgcagcaac tgcttttggg
    20221 tatgcaaggt tcgtgctcat caggcgcttt taacggcatt ccacgcgagc catctgcttc
    20281 aatgagaatt acgtcacact ctggtcgttg caccagtgca tcaatcgctt ctggcgtaaa
    20341 tccctgcact tttccctggt tcgctttcca gctgtgaaaa caaaatgaaa tgggggatgt
    20401 aagagacgca tgaggaagca tggcgggatc acgacagaaa accacgggcc agtgagacgt
    20461 tggcataaac atatgtgtcg tcgtcgtgat taacacacgc ctgccgctag cctggagcag
    20521 ttctgccagc caaaaaagca agctggtttt ccccccagca ccaacaacag aaatcacagt
    20581 gggacgtttc tgcgcaccta aatcaatgac taatgccgat gggtcaacta tacttttcac
    20641 gagtctttat gacctctctg ggataaatta tccccaactt aatccatcag gaagtaacgc
    20701 aattatcagg cgttattagc ccctataaat aatggaacca ctatgtcagc catcgactgt
    20761 ataattaccg ctgccggatt atcatcaaga atggggcaat ggaaaatgat gttaccctgg
    20821 gaacagggaa caattcttga tacaagtatc aaaaatgcgt tgcagttttg tagccgaatt
    20881 attttagtca ccggctatcg tggtaatgaa ctgcacgaac gttatgcgaa ccagagcaat
    20941 atcactatta ttcacaaccc agattatgcg cagggtttac taacatccgt caaggccgca
    21001 gtacccgcag tgcaaacaga acattgtttt ctcacccacg gtgacatgcc aaccctcacc
    21061 atcgatattt ttagaaaaat ctggtcgtta cgaaatgatg acgcaatact gccactccat
    21121 aatggcatcc ccggccatcc gattttagtg tcaaaaccat gcttgataca ggcaatccaa
    21181 cgacccaatg tcaccaatat gcgtcaggca ctccttatgg gagatcatta ttccgtcgaa
    21241 atagaaaatg cagaaataat tttagatatt gatactccgg acgactttat tacagcgaag
    21301 gaaaggtata ctgaaattta gaaaacgtag ttaaacgatt gcgttcaaat atttaatcct
    21361 tccggcactc attatcataa tgataaggaa tcactgattt tgagaaaaga gaaaaattga
    21421 taaatgggct ggcaataata attttcagga gtgattttaa ttctcattta gcacctgtga
    21481 gttcattcac aaaaagccct taagtttcga gccgcctcac atttttttat atttcccgcc
    21541 aaacctggca agagtggtgc gattgttgct ctatccccct aaaccaccgg atttctcaac
    21601 accggtcact caatgatatc tgtataagct aaggagaggg ttatggggga tattatgcgt
    21661 cccattccgt ttgaggaact tttgacgcgc atatttgatg aataccaaca acaacgctca
    21721 atctttggta ttcccgagca acagttttac tcacctgtaa aaggtaaaac tgttagcgtc
    21781 ttcggtgaaa cctgtgccac tcccgtcggc cctgccgctg gcccgcacac gcagctcgcg
    21841 caaaatattg tcacttcctg gctgactggc ggacgtttca tcgaactaaa aaccgtccaa
    21901 attcttgacc gcctggagct ggaaaagccc tgtattgatg ccgaagacga gtgctttaac
    21961 accgaatggt ctaccgaatt taccctgctt aaagcctggg atgaatacct caaagcctgg
    22021 tttgccctgc accttctcga agcgatgttc caaccttctg attccggtaa atcgttcatc
    22081 tttaatatga gcgtcggtta caacctcgaa ggtattaagc aaccgccaat gcaacagttc
    22141 atcgacaata tgatggacgc atctgaccat ccgaaattcg cgcaatatcg cgatacgctg
    22201 aataaattac tccaggatga cgcattttta gcccgccacg gattgcagga aaaacgcgaa
    22261 agcttgcaag ccttacccgc tcgcatcccc accagtatgg tgcatggcgt caccctctcc
    22321 accatgcacg gctgtcctcc gcatgaaatc gaagccattt gccgctacat gctggaagaa
    22381 aaagggctca acacctttgt gaaacttaac ccgaccttac tggggtacgc gcgtgttcgt
    22441 gagatcctcg atgtctgcgg tttcggttac ataggcttaa aagaagagtc atttgatcac
    22501 gacctcaagc tgacgcaagc actggaaatg ctggaacgcc tgatggcact ggcaaaagaa
    22561 aaatcactcg gctttggcgt aaaactgact aacactctcg gcaccatcaa caataaaggc
    22621 gcactgcctg gtgaagagat gtatatgtca ggccgtgcgc tgttcccgct ctccatcaat
    22681 gttgcagcag ttctctctcg cgcctttgac ggcaaactgc ccatttctta ttccggtggt
    22741 gccagtcagc tgactatccg cgatattttt gatacaggta ttcgccctat tactatggca
    22801 accgacctgc tgaaacctgg tggctatctg cgtttaagtg cctgcatgcg cgagctggaa
    22861 ggctccgacg cctggggact tgaccatgtt gacgtcgaac gactgaacag actggcagca
    22921 gacgcgctaa ccatggaata cacccagaaa cactggaagc cagaagagcg tattgaagtg
    22981 gcagaagacc tgccgctgac cgactgctac gttgccccct gtgttactgc ctgcgcgatc
    23041 aagcaagata ttccggaata catccgtctg cttggcgaac accgctatgc cgacgcgctg
    23101 gaacttatct accaacgcaa cgccctgccc gccattaccg gtcatatttg cgatcaccag
    23161 tgccaataca actgtacccg cctggattac gacagtgcac tgaatatccg cgaactgaaa
    23221 aaagtcgcgc tggaaaaagg ttgggatgaa tataagcaac gctggcacaa accagccggt
    23281 tctggttcac gccatccggt tgccgtgatt ggtgcaggtc cggcgggtct ggctgcaggt
    23341 tacttccttg ccagagcggg ccatccggtt acgctgtttg aacgcgaagc caatgcgggc
    23401 ggcgtggtga aaaatatcat ccctcagttc cgtattcctg cagagttaat tcagcacgat
    23461 atcgattttg ttgccgctca cggcgtgaaa tttgagtatg gctgctcacc cgatttgacc
    23521 gttgaacagt taaaaaatca gggcttccac tatgttctga ttgccaccgg cactgataaa
    23581 aatagcggtg tgaaactggc gggcgacaac caaaatgtct ggaaatcact ccccttcctg
    23641 cgtgaataca acaagggtac agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg
    23701 ggtaacaccg caatggactg cgctcgtgcg gcgttacgcg ttccaggcgt aaaaaaagca
    23761 acgatcgttt accgtcgttc actgcaagag atgcccgcat ggcgcgaaga gtatgaagaa
    23821 gcgttgcacg acggcgtgga gttccgtttc ctgaataatc cggaacgttt cgatgctgat
    23881 ggcaccttaa ccttgcgcgt tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgtcgt
    23941 ccggttgaaa ccaacgaaac agtaacactg catgtagaca gcctgatcac cgccattggt
    24001 gaacagcagg atactgaagc cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg
    24061 ccagacgtcg accataatgg cgaaactcgt ctgactgacg tctttatgat cggcgacgta
    24121 cagcgcggac catcctccat tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc
    24181 atccttagtc gggaaaatat ccgttcccac cagaacgata aatactggaa caacgtcaat
    24241 ccggcggaaa tctatcaacg taaaggcgat atctctatca cgctggtgaa cagtgacgat
    24301 cgtgatgcgt ttgtcgcgca ggaagctgct cgctgccttg aatgtaacta cgtttgcagc
    24361 aagtgcgtgg atgtctgccc gaaccgcgcc aacgtctcca ttgcggtccc aggcttccag
    24421 aaccgtttcc agacgctgca cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag
    24481 ttctgtccgt ggaacggtaa accatacaaa gacaaaatca ccgtcttcag cctgtcgcaa
    24541 gactttgata acagcagcaa cccaggcttc cttgtggaag attgccgggt acgagtacgt
    24601 ctgaataacc aaagctgggt gttaaacatc gacagcgaag gtcagttcaa caacgtacca
    24661 ccggagctga acgatatgtg ccgcatcatc agccatgtcc accagcatca tcattatctg
    24721 ctgggccgcg tggaggtgta atcatgttga ttctgaagaa tgtcaccgca gtgcagttac
    24781 acccggcgaa agtgcaggaa ggcgttgata tcgctatcga aaatgatgtg attgtcgcta
    24841 tcggcgatgc cctgacgcaa cgctaccccg atgccagcta caaagagatg catggtcgga
    24901 ttgtgatgcc gggaattgtc tgctcgcaca accattttta ctcggggctt tcccgcggaa
    24961 ttatggcaaa catcgccccc tgcccggatt tcatctcaac gctgaaaaat ctctggtggc
    25021 ggctcgatcg cgcccttgat gaagagtcgc tctattacag cgggctgatt tgttccctgg
    25081 aagcgattaa gagcggatgt acatcggtta tcgatcacca tgcctctcca gcgtatatcg
    25141 gcgggtcgct ctccacattg cgcgacgcat ttttaaaagt tggcctgcgc gcaatgacct
    25201 gttttgaaac tactgaccgt aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa
    25261 acatccgctt tgcccgtcag attgatgagg cgaagaaagc agcaaccgag ccgtatctgg
    25321 tggaagcaca tatcggtgct cacgcgccgt ttaccgtgcc agatgccggt ctggagatgc
    25381 tgcgtgaagc cgtgaaagcc acaggtcgtg gtttgcatat tcacgctgcg gaagaccttt
    25441 acgacgtttc ctacagtcac cactggtacg gcaaagacct gctggcacga ctggcgcaat
    25501 tcgatctcat cgacagcaaa acgctggtcg ctcatgggct gtacttgtcg aaagatgaca
    25561 tcgccctact caatcagcgc gatgcgttcc tggtgcataa cgcccgttca aacatgaaca
    25621 accatgtcgg ctacaaccat caccttagcg acatccgcaa tctggcgttg ggaacggacg
    25681 gcattggttc ggacatgttt gaagagatga aatttgcctt ctttaaacat cgcgatgcgg
    25741 gtggtccgct gtggcctgac agttttgcca aagccctgac taacggtaac gaactgatga
    25801 gccgcaactt tgccgcgaaa tttggtcttc tggaagccgg ttacaaagcc gatttaacca
    25861 tttgcgatta caactcgccg acgccgctgc tggcagacaa tatcgccggg catatcgctt
    25921 tcggtatggg ctcaggcagc gttcacagcg tgatggtcaa tggcgtgatg gtctatgaag
    25981 accgtcagtt taactttgat tgcgattcca tttatgcaca agccagaaaa gccgctgcca
    26041 gtatgtggcg tcggatggat acgctggcat aaatgacaga tgccctcttc ccgcagggaa
    26101 gagggttaaa taaggaaaga agatgattga acaatttttc aggcccgact ctgtcgaaca
    26161 ggcgctggaa ctgaagcgcc gctaccagga tgaagccgtc tggttcgccg ggggcagcaa
    26221 actcaacgct acaccaaccc gtaccgataa aaagattgcc atttccttac aggatctgga
    26281 actggactgg gttgactggg ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca
    26341 gccactgcgt gatgcgcgat ttattcctgc agcactgcgt gaagctctcg gttttgttta
    26401 ctcacgccat gttcgtaatc agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga
    26461 gtcggtgctg cttcctgtcc tgctggcact ggatgctgaa ctggtttttg gcaacggcga
    26521 aacgctgtca atcgaggact acctggccag cccatgcgat cgcctgctaa ccgaaattat
    26581 cattaaagat ccgtatcgca cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt
    26641 aaccgtcgtg acggcagccg ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga
    26701 tggcgtcgcc agtaaagcac tgcgtctgca tgatgtcgaa aaacaaaatc tggaaggcaa
    26761 tgcacttgaa caggctgtcg ccaacgccat tttcccgcag gaagatttgc ggggcagcgt
    26821 ggcctataaa cgctatatca cgggagttct ggtagccgac ctgtatgccg actgccaaca
    26881 ggctgaggag gaagccgtat gatcatccac tttactttaa atggcgcgcc tcaggagcta
    26941 accgttaatc caggcgaaaa cgtgcaaaag ctgttgttta acatgggaat gcactctgta
    27001 cgcaacagtg atgatggctt cgggtttgct ggttctgacg caataatctt taacggtaat
    27061 atcgttaacg cgtccttgct tattgccgca cagttagaga aggcagatat tcgtaccgca
    27121 gaatctctgg gcaaatggaa cgagttaagt ctggttcaac aggcaatggt tgatgttggc
    27181 gtggtgcagt ctggttataa cgatccagct gcagctctga ttatcaccga tcttctcgat
    27241 cgcatcgccg cacctacccg cgaagagatc gacgacgcgc tttctggttt gttcagccgc
    27301 gatgctggct ggcagcaata ctatcaggtc attgaactgg cggttgcacg taaaaataat
    27361 ccgcaggcca ccattgatat cgctccgact ttccgtgacg acctagaagt cattggtaag
    27421 cattatccta aaactgatgc cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac
    27481 cgcgtaacgg ctgacgcctg cgtcattaaa gtgttacgta gcccacacgc tcacgcactg
    27541 attactcatc tggatgtcag caaagctgaa gccttaccgg gcgtcgttca cgttattact
    27601 cacctgaatt gcccggatat ctactatacc ccgggtggtc agagcgcacc ggaaccgtca
    27661 ccgcttgacc gccgtatgtt cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg
    27721 gtcgtcgcag aaagtgaaga aattgcgctc gaagcactga agctcatcga cgttgaatat
    27781 gaagtgctta agccggtaat gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg
    27841 cacgatgaac cggtggtgta tgttgctggt gcgccagata ctctggaaga cgataacagc
    27901 catgcagccc agcgcggcga gcatatgatc atcaacttcc cgatcggttc tcgccctcgc
    27961 aaaaatatcg ccgccagtat tcatggtcat attggcgata tggacaaagg ctttgccgat
    28021 gccgatgtga tcattgagcg aacctataac tcaacgcagg cacagcagtg cccgactgaa
    28081 acacatatct gctttacccg gatggacggc gatcgtctgg ttatccacgc ctccacccag
    28141 gtaccatggc acttacgccg ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt
    28201 catgtcatta aagagcgagt tggcggcggt tttggttcca aacaggacat cctgctggaa
    28261 gaagtgtgcg cctgggcaac ctgcgtgacc gggcgtccgg tactgttccg ctacacccgt
    28321 gaagaagagt ttattgctaa cacctctcgt cacgtcgcga aagtcaccgt caaactggga
    28381 gcgaaaaaag atggtcgcct gacggcagtg aagatggatt tccgcgccaa cactggccct
    28441 tacggcaacc actcactcac cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat
    28501 ccgtgcgata acgtcgattt ccaggtcacc acctactaca gcaacatttg cccaaatggt
    28561 gcttatcagg gttatggcgc accgaaaggt aacttcgcta tcaccatggc attagcggaa
    28621 ctggctgaac agttacagat cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa
    28681 gggcaagagc tgaaaattct cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct
    28741 tccgccgcca gctgtgcact ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg
    28801 tcgtcaccga aaccgcaaaa tggtgactgg cacatcggtc gcggcgtcgc cattatcatg
    28861 cagaaatcgg gtatcccgga tatcgatcag gctaactgca tgatcaaact ggaatcggac
    28921 ggcaccttta tcgttcattc tggcggtgcg gatattggta ctggtctgga tactgtagtg
    28981 acgaaactgg cagcagaagt gctgcactgc ccaccgcagg acgtgcatgt tatctccggt
    29041 gataccgatc atgcgttgtt tgataaaggc gcatatgcct cgtccggtac ttgcttctcg
    29101 ggtaacgcgg cgcgtttggc agcggaaaat ctgcgggaga aaattctgtt ccacggcgcg
    29161 caaatgttgg gtgagccagt ggcagatgtt caactagcaa cgccgggcgt cgtgcgcggc
    29221 aagaaaggcg aagttagttt cggggatatt gcccataaag gcgaaaccgg caccggcttt
    29281 ggttcactgg tgggaactgg cagttatatc acgcctgatt tcgctttccc gtatggcgca
    29341 aacttcgctg aagttgccgt caacacgcgt acgggtgaaa tccgcctgga taaattctac
    29401 gccttgctgg actgcggtac accggtcaat ccagagttag cgttgggaca aatctacggt
    29461 gccaccctgc gtgctatcag ccacagtatg agcgaagaga tcatttataa cgccgaaggt
    29521 cacccgttaa cgcgtgattt acgcagttac ggcgcaccga aaattggtga cattccgcgt
    29581 gatttccgcg ctgtgttggt gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg
    29641 atctcggaaa ttggtgtaaa tggcgcagct ccggcgattg ctaccgcaat tcacgatgcc
    29701 tgcggcatct ggttacgcga atggcatttc acaccggaga aaatactcac cgcgctggaa
    29761 aaaatataag taataaatgg ccgcttcggc ggcctgtttc tctgtttgaa cgatgactaa
    29821 atagtgactg cgaaaatgag ataagccgtt ctcaaggcgt cctgcgcccc aaattcgtgg
    29881 gaatttattt tactaattca gatgatcaaa tttactttaa aaggagtgaa gggatgtctg
    29941 atataaacca tgcaggttct gaccttatat ttgaactgga ggatcgccct ccctttcatc
    30001 aggctctcgt tggtgccatt acccatctgt tggcaatttt cgttccgatg gtaacccccg
    30061 cgttaatcgt gggtgcggcc ttacagcttt ccgctgaaac aaccgcctat cttgtttcta
    30121 tggcgatgat cgcctctggt attggtacct ggttacaagt aaaccgctac ggcatcgtcg
    30181 gttctggcct actctcaatt cagtcagtca atttttcatt tgttacggtc atgattgcgc
    30241 tgggcagcag catgaaaagc gacggttttc acgaagagtt aatcatgtcg tcgcttctcg
    30301 gcgtctcctt cgttggcgca tttctggttg tcggatcttc atttatcttg ccctatttac
    30361 gtcgggttat tacgcctacc gtcagcggta ttgtggtact gatgatcggc ttaagcctga
    30421 ttaaagtcgg cattatcgat tttggtggag gatttgcagc caaaagcagc ggtacgttcg
    30481 gcaattacga acatctcggc gttggtttat tggttttaat tgtggtgatc ggctttaact
    30541 gctgtcgcag tccgttgcta cgcatgggag ggatcgccat tgggctatgt gtcggctata
    30601 tcgcatcgtt atgcctgggc atggtggatt tcagcagtat gcgcaatttg ccgttaatca
    30661 ccatcccgca tccgttcaaa tacggcttta gttttagctt ccatcagttc ctggtggttg
    30721 gcacgattta tctgcttagc gtgctggaag cagtcggcga tatcaccgcc acggcaatgg
    30781 tttcccgccg ccccattcag ggggaagagt atcagtcccg actgaaaggc ggcgtgctgg
    30841 cagatggtct ggtttctgtt atcgcctccg ctgtcgggtc attaccctta accacgtttg
    30901 cgcaaaataa tggggttatt cagatgactg gcgtcgcttc acgttatgtc gggcgaacca
    30961 tcgcggtaat gctggttatc ctcggtttgt ttccgatgat tggcggcttc ttcacgacca
    31021 ttccctcggc agttctggga ggcgcaatga cgttgatgtt ttccatgatt gccatcgctg
    31081 ggattcgcat aatcatcacc aacggtttaa agcgccgaga aacacttatt gtcgccactt
    31141 ctttaggttt aggacttggc gtctcctacg atcccgaaat ttttaaaata ttgccagcct
    31201 ctatttatgt actagttgaa aaccctattt gtgctggcgg gttaactgcg attttattaa
    31261 atattatcct ccctggtggc taccgacagg aaaacgttct gcctggtatt acctcagcgg
    31321 aagagatgga ttaacagtaa aggagtcaat gatgtcagga gaacacacgt tgaaagcggt
    31381 acgaggcagt tttattgatg tcacccgtac ggtcgataac ccggaagaga ttgcctctgc
    31441 gctgcggttt attgaggatg gtttattact cattaaacag ggaaaagtgg aatggtttgg
    31501 cgaatgggaa gacggaaagc atcaaattcc tgacactatt cgcgtgcgcg actatcgcgg
    31561 caaactgata gtaccgggct ttgtcgatac acatatccat tatccgcaaa gtgaaatggt
    31621 gggggcctat ggtgaacaat tgctggagtg gttgaataaa cacaccttcc ctactgaacg
    31681 tcgttatgag gatttagagt acgcccgcga aatgtcggca ttcttcatca agcagctttt
    31741 acgtaacgga accaccacgg cgctggtgtt tggcactgtt catccgcaat ctgttgatgc
    31801 gctgtttgaa gccgccagtc atatcaatat gcgtatgatt gccggtaagg tgatgatgga
    31861 ccgcaacgca ccggattatc tgctcgacac tgccgaaagc agctatcacc aaagcaaaga
    31921 actgattgaa cgctggcaca aaaatggtcg tctgctatat gcgattacgc cacgcttcgc
    31981 cccgacctca tctcctgaac agatggcgat ggcgcaacgc ctgaaagaag aatatccgga
    32041 tacgtgggta catacccatc tctgtgaaaa caaagatgaa attgcctggg tgaaatcgct
    32101 ttatcctgac catgatggtt atctggatgt ttaccatcag tacggcctga ccggtaaaaa
    32161 ctgtgtcttt gctcactgcg tccatctcga agaaaaagag tgggatcgtc tcagcgaaac
    32221 caaatccagc attgctttct gtccgacctc caacctttac ctcggcagcg gcttattcaa
    32281 cttgaaaaaa gcatggcaga agaaagttaa agtgggcatg ggaacggata tcggtgccgg
    32341 aaccactttc aacatgctgc aaacgctgaa cgaagcctac aaagtattgc aattacaagg
    32401 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
    32461 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
    32521 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
    32581 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
    32641 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
    32701 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
    32761 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
    32821 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
    32881 ggttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
    32941 ctgatggggt tgtgggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
    33001 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actgggcgcg
    33061 gtctttctga tgggcgtcat cttcaccgct atttccgtaa ccggtgtgcg tacctggatc
    33121 ttacgtaatt taccgatggg tatcgctcac ggtacaggta tcggtatcgg cctgtttctg
    33181 ctgctgattg ctgctaacgg tgtgggtatg gttatcaaaa acccgattga aggcttgcca
    33241 gtggcgctcg gtgcgtttac ctccttcccg gtgatgatga gcttgctggg gctggcggtc
    33301 atcttcggtc tggagaagtg ccgcgtaccc ggcgggatct tgttggtgat tatcgcaatt
    33361 tcgattatcg gcttaatctt tgacccagcg gtgaaatacc acggtctggt ggcgatgcca
    33421 agtctgactg gcgaagatgg taagtctctg attttcagcc tcgatattat gggcgcactc
    33481 cagccaaccg tacttccgag tgtactggca ttggtgatga ccgcagtgtt cgacgccact
    33541 ggcaccatcc gtgccgtcgc cggtcaggcg aatttgttgg ataaagacaa ccagatcatc
    33601 aatggcggca aagccctgac cagtgactca gtaagttcaa tattctccgg tctggtgggc
    33661 gcagcgcccg cagcggttta tatcgaatca gcggcaggaa ccgccgccgg gggtaaaaca
    33721 gggttgaccg caaccgtagt gggggcgtta ttcctgctga ttctgttctt atcaccgctg
    33781 tcatttttga tccctggtta cgccactgca cccgctctga tgtacgtggg tttgctgatg
    33841 ttaagtaacg tctcgaagct ggatttcaac gattttattg acgcaatggc tggcctggtg
    33901 tgtgccgtgt tcatcgttct gacttgtaat atcgttaccg gtattatgct gggctttgtg
    33961 acactggtcg taggccgtgt ctttgctcgc gaatggcaaa agttgaatat tggtacggtg
    34021 atcattactg ccgcactggt cgcattttac gcgggtggtt gggcaatcta atagattctc
    34081 cgcgcctttc agcgcggaga tttctttcac agaggattct caccgctggc aacaatacgc
    34141 tgctggcgga ctcgcctgag ttctctttca gtaagcaact gcagcgcctg tgtcgggcaa
    34201 gattcaacac aggctggccc ctcttcccgc tgttcacaga gatcgcattt cacaatttgt
    34261 tgtcgggtat cccccggcaa cgactgaatg gtgatcattc caaacgggca cgcactgacg
    34321 caactctgac agccaataca acgggcagaa ttggtctgca ccacctgctc gcccatcgtc
    34381 agcgccccca cggggcaagc gccaacacaa ggggcgtttt cacactgatg gcacatcacc
    34441 ggcgcactga tgctgtccag tcgctgtacc ttcagccggg gtaaaaagac atcggcattc
    34501 aactcctgtt ctgaagggtg agcgaccaca caggctactt cacaagtacg gcagccaata
    34561 cagtcagccg gattaacgat aattaacgat ttcatcacga tgccttcgta tcaaacagag
    34621 ttaacatatc gcgcgccgcc tgccttcctg cggccattgc agtgacaacc agatccgcgc
    34681 catgaactgc atccccacca gcaaagactt ttttcagatg cgtctgggta ggtaaatacc
    34741 cgacgtcacc ggtttgaatc aggccccatt tatcgagttt aattccgctg ccctgcaacc
    34801 acggcatggc atgcgcctgg aaaccaaagg ccataatgag aacatcggcg ggcaattcaa
    34861 actctgaacc cgcaaccgga cgaggacgac ggcgaccatc cggccccggc tcacccatgg
    34921 cggtacgaat caggcccacc gcagttaaac gcccatcttc gtcacaagcg atatattgcg
    34981 gttgaacatt gaactgaaac tcgacacctt cctcgcgcgc attgaccacc tctttgcgcg
    35041 agcccggcat actgacttca tcacgacgat acgcgcaggt cacgctggcg gcattgaggc
    35101 ggatggaagt ccgcaaacaa tccattgtcg tatcgccacc gcccaatacc acgactcgct
    35161 taccttccac gtccgtcagc ggatactctt cagactccgg caaccccatg agctggcggg
    35221 tatgggcagt caagaacggt agagcctgaa tgacaccggg cgcatcttca tgcggcagat
    35281 cagctcgcat catcccgtaa gtccccacgc cgatgaaaac tgcatcatat tcagaagtta
    35341 aatcgctaaa ggtaatatcg cggccaattt cacagttaag atgaaagtcg attcccattg
    35401 cggtgaatat ctctcgccgc tggcttaata ccgttttatc gagtttgaaa ggaggtatgc
    35461 caaaagtcag cataccgcca atttctggat ggcgatcaaa gacatcaacc tgaactcctg
    35521 cgcgcgccag aatatcagca caccctaacc ctgctggccc agcgccaatc accgccactt
    35581 tttcgctacg gggaacaact ttgctgacat cgggacgcca gcccatcgcc agcgcggtat
    35641 cggtgatgta gcgttccaga ttaccgatag agactgcgcc cgagtgatct ttcaaagtac
    35701 atgcgccttc acaaagacgg tcctgtggac ataccctgcc gcaaatttct ggtaaggaac
    35761 tggtctggtg gcaaagttct gccgcttcaa taatctttcc ttcctgtacc agacggatgt
    35821 aatccggaat agcgttatgc agcgggcaat gccagttgca gttagctttt tcggcacaat
    35881 aaacacagcg gtcactctca taagtcgctt gcagtggatc cagcccgcaa tatatttcgc
    35941 caaagtgggt tttccgttca ctcgctgaaa ttttatctgc gcctttacgc gagttaacgg
    36001 ggagcaacgc tgcactgcga gatggctgag cgtctgatga cgcttttcct gctgccgttt
    36061 tgcgctggcg agccaccttt atccactgta accctttatc gtccatcagt cgtaacgcct
    36121 gtgttgggca gacttcaata caggcttgcg tgccggaact gcgctggtta caaaggtcgc
    36181 atttctgcgc aatcgtatcg accatctcaa cgacgccaaa ggggcaagcg attgcgcatc
    36241 ttttacagcc aatacatttt tgctcgtcca gttgtacgct atcggactgg aaagtcagag
    36301 cattaaccgg acaagccgta acgcaagggg cattgttgca gtgatggcag gccaccggat
    36361 tcgcagcctg gcctttccct acaacgtgga tacgcggtcg aaagtcactg tgactcagcg
    36421 gccagttttc ttgattgtgt gccaccgcac aggcaatttc acaagcatga cagcctatac
    36481 attccgcagc ttcagcagcg ataaacttat tcatttgcat ccctttcatt taatgagtta
    36541 tgtcttctca aatttcgcga tgcaataaac gggcatatat taggtagcat gaccgtttta
    36601 attataattt tgcgactgag ttcaaattat tgcccctcaa tctatgccgc tttcattatt
    36661 tcaacattgc cgaccaaaag agcgctatcc agataaaaac gattaaatgc gagagtgcga
    36721 catgccagaa tgattaatga aatatcaata tgagaaaatt acagataatt tcattgtcag
    36781 gttctcttct cattttcgtg gtttaaccac gattatgtga ttgcctccgc aattcctgtc
    36841 tctaactccc cttcctcgca aaaactggca ctccacgagc atgtgtttag acagtttcat
    36901 taacgtaaac ggttgctttt tactctggcg ggcgaaagga gaaacactga tgagcgccat
    36961 agattcccaa cttccctcat cttctgggca agaccgccca actgatgagg ttgaccgcat
    37021 attatcacca ggaaagctga tcatactcgg tctgcaacac gtccttgtca tgtacgcagg
    37081 tgcagtcgct gttcctctta tgattggtga ccgactgggc ctctcaaaag aagctattgc
    37141 gatgctcatt agctcggatc tcttttgctg cgggatcgtc acattattgc aatgtatcgg
    37201 tatcggccgc tttatgggga tccgcctgcc ggtgattatg tcggtgacct ttgctgctgt
    37261 aacaccaatg atagccattg ggatgaaccc ggatatcggc ctgctgggga tatttggtgc
    37321 cactatcgcc gcgggtttta tcaccacatt attagcgcca cttatcggtc gcttgatgcc
    37381 tttattcccg ccactggtta ccggtgtggt tattacttct atcggactta gcatcattca
    37441 ggtgggtatt gactgggccg ccggaggtaa agggaatccg caatatggta atcccgttta
    37501 tttaggtatc tcctttgctg tcttaatttt tatcttgctc attactcgct atgcgaaagg
    37561 atttatgtcc aacgtcgccg tattactggg gattgtattt ggctttttac tttcgtggat
    37621 gatgaatgaa gtcaatttat ccgggctaca tgatgcttca tggtttgcga ttgtcacgcc
    37681 gatgtcattt ggtatgccga ttttcgatcc cgtttccatt ctgaccatga ctgccgtgtt
    37741 aatcatcgtg tttatcgagt cgatggggat gttcctggca ctgggtgaaa tagtcggtcg
    37801 taaactctct tcacacgata ttattcgcgg gctgcgtgtc gatggcgtag ggacaatgat
    37861 aggcggaacg tttaacagct tcccccacac gtcattttca caaaacgttg gcctggttag
    37921 cgtgacgcgc gttcatagcc gctgggtgtg tatttcttcg ggaattatat taatcctgtt
    37981 tggcatggtg ccaaaaatgg cggtgctggt cgcctccatt ccgcaatttg tgctgggcgg
    38041 tgctgggctg gtgatgttcg gcatggtact ggcgacaggg attcgaattc tgtcgcgctg
    38101 taactacacc accaaccgtt acaacctcta tattgtggcg atcagtctcg gcgttggcat
    38161 gactccgaca ctctctcacg atttcttttc taagttaccg gccgtactgc aaccgctgct
    38221 acatagcggc attatgctcg caacccttag cgccgttgtg ctgaacgtct tctttaatgg
    38281 ctatcagcat catgctgacc tggtgaagga atccgtctct gataaagatt taaaagtcag
    38341 gacagtacgt atgtggcttc tgatgcgcaa gctgaagaaa aatgagcatg gagaataata
    38401 tgaatctttt aatgcgcgct atattcagtc tgctgttgct ttttactctc tctattcctg
    38461 tcatttctga ctgtgttgca atggccattg aaagtcgctt caaatatatg atgctacttt
    38521 tttaaatggt ttttacctgt cggcatccgc tcaaaacggg cggttgtcga taaacgctca
    38581 cttggttaat catttcactc ttcaattatc tataatgatg agtgatcaga attacatgtg
    38641 agaaattatg caaacggaac acgtcatttt attgaatgca cagggagttc ccactggtac
    38701 gctggaaaag tatgccgcac acacggcaga caccctctta catctcgcgt tctccagttg
    38761 gctgtttaat gccaaggggc aattattagt tacccgccgc gcactgagca aaaaagcgtg
    38821 gcctggcgtg tggactaact cggtttgtgg gcacccacaa ctgggagaaa gcagcgaaga
    38881 cgcggtgatc cgccgttgcc gttatgagct tggcgtggaa attacgcctc ctgaatctat
    38941 ctatcctgac tttcgctacc gcgccaccga tccgagtggc attgtggaaa atgaagtgtg
    39001 tccggtattt gccgcacgca ccaccagtgc gttacagatc aacgatgatg aagtgatgga
    39061 ttatcaatgg tgtgatttag ctgatgtatt acacggtatt gatgccacgc cgtgggcgtt
    39121 cagtccgtgg atggtgatgc aggcagccaa tagtgaagca agaaaattgt tgtctacttt
    39181 cgcgcagcac aattaaaaaa ccccgacatt tgccggggtt gtgagcataa cgtaatgctt
    39241 attttaccgg acgcatcgcc gggaacagaa taacgtcgcg gatggtatgg ctgttggtga
    39301 acagcattac catacggtcg atgccaatcc ccagacctgc tgtcggcggt aagccatgtt
    39361 ccagtgcggt aacgtaatct tcatcgtaga acatcgcttc gtcgtcacct gcgtctttcg
    39421 cggcaacctg atccaggaag cgttgcgcct gatcttccgc gtcattcagc tcgctaaagc
    39481 cgttaccgat ttcacgacca ccgatgaaga actcaaagcg gtctgtgatt tccgggttaa
    39541 tgtcgttacg acgcgccagc ggagaaactt ctgccggata ttcagtaatg aaggtcggct
    39601 gaatcagatg tgcttctgcc acttcttcga agatctcggt aacgatacgg cccagacccc
    39661 agctcttctc aacgtggata ccgatagatt cagcaatcgc tttcgcagag tcgaagttat
    39721 ccagatccgc catgtcggtt tccgggcgat atttcttgat cgcttcacgc atggtcagtt
    39781 tttcgaacgg tttaccgaag tccagcgtca cgtcgccgta ggtcacttcc gtcttaccga
    39841 gaatatcctg tgccagagta cggaacagcg attcggtcag ctcgatcaga tctttgtaat
    39901 ctgcgtaagc catgtagagt tccatcatgg tgaactctgg gttatgacgt acggaaatac
    39961 cttcgttacg gaagttacgg ttgatttcga atacacgctc gaagccaccg accaccagac
    40021 gcttgaggta cagttccggc gcgatacgca ggtacatgtc gagatccagc gcgttatggt
    40081 gggtgataaa cggacgcgca gcggcaccgc ctgggatcac ctgcatcatc ggcgtttcaa
    40141 cttccataaa gccgcggttc accatgaact ggcgaatacc agagaggatc tgcgagcgta
    40201 ctttaaaggt gttacgggat tcatcgttag agatgagatc cagataacgc tgacgatagc
    40261 gcgcttcctg atcctgcaag ccgtggaatt tatccggcag cggacgcagt gctttggtca
    40321 gcagacgcag ctcggtacag tggatagaca gttcgccggt tttggttttg aacagtttac
    40381 ctttcgcgcc gaggatgtcg ccgaggtccc attttttgaa ctgctcgttg taaacgcctt
    40441 ccggcagatc gtcacgggca acgtacagct gaatgcggcc acccacatcc tgcagggtaa
    40501 cgaaagacgc tttacccata atacggcggg tcatcatgcg gccagcaacg gcgacttcga
    40561 tgttcagcgc ttccagttct tcgttctctt taccgtcgaa ttctgcgtgc aattggtcag
    40621 aggtatgatc gcgacggaaa tcgttcggga aggcaatccc ctgctcgcgc aggttcgcca
    40681 gcttctcacg acgcgttttc agttcattgt taagatcgac taccgcgtca gcgccctgtg
    40741 cgtgttgttc agacatgttg gttcctcata accctgcttt caaacttgct tcgataaatt
    40801 gatccaggct gccgtccagc acggcctgcg tgttgcgggt ttctaccccg gtacgcagat
    40861 ctttaatgcg ggagtcatca aggacataag aacgaatctg gctgccccag ccgatgtcgg
    40921 atttgttatc ttccatcgcc tgtttctcgg catttttctt ctgcatctcc agttcataaa
    40981 gcttcgcttt catctgcttc atggcctggt ctttgttctt gtgctgggaa cggtcgttct
    41041 ggcattgggt cacgatcccg gttgggatgt gggtaatacg caccgcagat tcggtacggt
    41101 taacgtgctg accgcccgcg ccggacgcgc gataaacgtc aatgcgcaga tccgccgggt
    41161 tgatttcgat atcaatatca tcatcaactt ccgggtaaac aaacgcagag ctgaacgatg
    41221 tgtggcgacg accgccggag tcaaacgggc ttttacgcac caggcggtga acgccagttt
    41281 ctgtacgcag ccagccgtaa gcgtaatcgc cggagatttt gatcgtcacg gatttaatac
    41341 ccgccacttc accttccgac tcttcgatga tttcagtttt gaaaccacgc gattctgccc
    41401 agcgcagata catacgctca agcatgctcg cccagtcctg tgcttccgta ccgccagacc
    41461 ccgcctgaat atcgaggtag cagtcggcgc tgtcatattc gccagagaac atacggcgga
    41521 actcaagctg cgccagtttt tcttccaggg cgtcgagttc agcaacggct tcgttaaagg
    41581 tttcttcgtc gtcagcttct acagccagtt ccagcagacc agaaacatct tccagcccct
    41641 gtttcatttg gtcgagggtg tcgacaacgg cttcgaggga ggaacgctct ttacccagcg
    41701 cctgtgcgcg ttcgggttcg ttccagacat ccggctgttc cagctcggcg tttacttctt
    41761 ccagacgctc tttcttggcg tcgtagtcaa agataccccc taagaacgtc ggagcgttcc
    41821 gtgaggtcct gaatgcgatt atttaccgga ttaatttcaa acatggtctg atttctttta
    41881 ttgagctagt caaaatgcgg tgataagagc gggattgtac ccaatccacg ctctttttta
    41941 tagagaagat gacgctaaat tggccagata ttgtcgatga taatttgcag gctgcggtta
    42001 ccgcgaaact cgttgatatc gagcttgtaa gccagttgca cttcgcgcac gccgttatcc
    42061 ggccagagag cggtatcgac attaaaagca ataccatcca gcagtggacc gccgccgacc
    42121 ggttcgacca tcaccttcaa atgacgttcg cccaccagcc gctgttgcag cagacggaaa
    42181 tgaccgtcaa acagcggctc cgggaacatc tgcccccacg ggccagcatc gcgcagcagc
    42241 tgcgccactt ccatggtcat ttcggccggg cttaacggac cgtctgacac cacttcgcct
    42301 tgcaatagcg aagggtccag ccactcagta accagttcgc caaaccgttg ttgaaagagt
    42361 tcgaatttat cctcttccag cgacaaaccc gccgccatcg catgaccgcc aaacttcagc
    42421 atcatgccag ggtagagtgt gtctaatcgc tccagtgcat cacgcatatg cagcccctga
    42481 atggagcgac cggaaccttt cagcgtacca tcacctgctg gcgcaaaggc gataaccgga
    42541 cggtgaaaac gctctttgat gcgcgaagcc agaataccga caacgccctg atgccattcg
    42601 gggtgataca ttgccagccc gccgggtagc gtgtcgcgac ttcgctccag tttctcgcac
    42661 agggtcaggg cttcaacttg cattccttgt tcgatctctt ttcgcgtctg gtttagcgca
    42721 tcgagttcat ttgccagcac gcgcgcttcg ccgatgttgt cgcacagcaa cagcgccaca
    42781 ccgacggaca tatcgtccag tcgcccggca gcattgagac gtggccccag cgcaaaacct
    42841 aaatcgctgg cggcgagttt ttgcgcatcg cgatttgcca cttccagcag cgctttaatc
    42901 cccggacggc actttccggc acggatgcga ctcatcccct gccaggtcag aatgcgatta
    42961 ttagcgtcca gcggcacgac gtccgccact gttcccagcg cgaccagatc cagcagttct
    43021 gccaggttag gaattgcgat gccacgctca tcaaaccagc cctgatcgcg caaaaaggtg
    43081 cgcagcgcca gcatcagata aaacgccaca cccacgcctg ccagtgattt cgacgggaaa
    43141 ttacagtcgc gcaagttagg gttaatgatc gcttccgccg cgggtaatgt ttcgcctggc
    43201 aaatggtgat cggtaacaat aaccggaatg cccaacgagc gagcgtgtac aacccccgca
    43261 tgggaggaaa taccgttatc caccgtgaca attaactgcg cgccacgggc atgggcctga
    43321 tcgaccactt ccgggcttaa gccgtaaccg tcttcgaaac ggtttggtac caggtagtcg
    43381 atattgctgc aaccaagcga gcgcatcgcc agcacgctta gagccgtgct ggtcgcgccg
    43441 tcggcgtcga aatcaccgac cacaataatc cgcgttcctt cgcgaaaagc gttgtaaagg
    43501 atctcaacgg ccttttcgac accgctcagt tgctgccagg gcaacatacc tttaacactg
    43561 cgttccagtt cttgcgcact gcgcacgccc cggctggcgt ataaacggcg cagcaaggga
    43621 ggcaattcag cgggcaagtc tgccgtttca tcgacttcac ggcgacgaag ttgtatctgt
    43681 tgtttcacgc gaattattta ccgctggtca ttttttggtg ttcgtcgagg aattctttca
    43741 tctctttcgg cggctggtaa cccggaacaa gtgtgccatt gctcagcaca actgccggag
    43801 taccgctaac gccaagctgg acgccaagtg cgtaatggtc ggcaatatcc acgtcgcagc
    43861 tggctggtgc ggcgctttta cctgccatca catcatcaaa cgctttgttt ttatctttcg
    43921 cacaccagat agctttcatt tctttctctg catcgctgtc cagcccctgg cgcgggaaag
    43981 caagataacg cacggtgatc cccagcgcgt tatagtctgc catttgctca tgcagtttgt
    44041 ggcagtaacc acaggtaata tcagtaaaca cggtgatgac gtgtttttcc tgcggcgctt
    44101 tataaacgat catctctttt tcaagcgcat tcaactgctt taacagcatc ttattggtga
    44161 cattgaccgg agccgtgccg ctaacgtcat acattggccc ctgaatgata tgtttaccat
    44221 catcggtgat gtacaacacg ccgctgttag tcagaactgt cttcatgcca gctacgggcg
    44281 cgggctgaat atcgctgctt ttgatgccca ttttggctaa cgtttgttga attgccgcgt
    44341 catcagcctg agcaaaacct gaaaacgccg ctaacaaagt aaacaacata aaacctttct
    44401 tcataaatct tcccgttctt ttcagacatc acgcccgcgg gtgatgctgt tgatgaagtt
    44461 gccgcagacg ctcggtagcg acatgcgtat aaatttgcgt ggtggagagg tcgctgtggc
    44521 ccagtagcat ctgcaccacg cgtaaatccg caccatgatt taataaatgg gtggcaaaag
    44581 cgtgacgcaa tacatgcggt gacagctttt cgctgtcgat acccgccagc acagcataat
    44641 gtttaatgcg gtgccagaag gtctgtcgcg tcatctgctg cgcacgctgg ctgggaaaca
    44701 acacgtcaat tgacacacca ttcagcagcc acggacgccc atgttccaga taggtttcca
    44761 gccagtaaac cgcctcttca cctaacggta ccaggcgctc tttgttgcct ttaccaatga
    44821 cccgcactac gccctgacgc aggctgatat cactcattgt cagtccgacc agttcagaga
    44881 cacgcagccc ggtagcatac aacacttcaa gcatggcttt atcgcgtagc tccagtggct
    44941 gatcaattaa tggtgcctgt aataaacgtt cgacctgcgc ttcgcttaaa tcttttggca
    45001 aacgctgggg caatttcggt gaagcgagat gcgcactggg atcgtcttca cgaaactttt
    45061 cgcgataaag atactggaac aatcggcgca ctgcactcag caaacgcgct gagctggtgg
    45121 ctttataccc gccctccagc cgttctgcca gtaatgcctg caaatcgtca ctttgcgccg
    45181 tcgccagcgt caacccgcgg tgatgcaacc actccaccat cattgacaga tcgcgacggt
    45241 aagcgttcaa cgtattttca gccagatttt tttccagcca cagagcatca agaaactgct
    45301 cgatgcgtgc cagatcctgt ttcacttgcg ccccttatgg tcactcattt gatccattat
    45361 gccttattgt gccgtgacta aagcgattct gatacactag ccgcaaaagc cacagcagaa
    45421 tcgagaagct tacgttatga atatgggtct tttttacggt tccagcacct gttacaccga
    45481 aatggcggca gaaaaaatcc gcgatattat cggcccagaa ctggtgacct tacataacct
    45541 caaggacgac tccccgaaat taatggagca gtacgatgtg ctcattctgg gtatcccgac
    45601 ctgggatttt ggtgaaatcc aggaagactg ggaagccgtc tgggatcagc tcgacgacct
    45661 gaaccttgaa ggtaaaattg ttgcgctgta tgggcttggc gatcaactgg gatacggcga
    45721 gtggttcctc gatgcgctcg gtatgctgca tgacaaactc tcgaccaaag gcgtgaagtt
    45781 cgtcggctac tggccaacgg aaggatatga atttaccagc ccgaaaccgg tgattgctga
    45841 cgggcaactg ttcgtgggtc tggcgctgga tgaaactaac cagtatgacc ttagcgacga
    45901 gcgtattcag agctggtgcg agcaaatcct caacgaaatg gcagagcatt acgcctgatc
    45961 tcactgacgg cttagcgcat atgctttgcc gtcatcttta tctttgcgtc tcttgttgca
    46021 acaaaatccg ccgtaaatcc cgccattcgg cttcgtccat gctgtcggct gccagccata
    46081 aatgttgccg tttaccgcca tcagaacgta aacgcagcat catgccgctc ttaatcatcc
    46141 acggtgcttt gacgatgctc cactcctgcc cttgccaacg caaacgcccg tccatcaaca
    46201 agcgaatttc cccctggcga gcattaatac gccgctggct gcgaacgcaa tcaaacacca
    46261 ccagcgaaag taacaccatc cataacgggg tgtaactgag tggccagggc atgagtaaaa
    46321 taacagcggc aaccagccca tgaatcagca aggaaagcca ctgtgcgcgc caggagacgc
    46381 gcaaatcaga ttgccacagg accacgttcc cggttccgtg tctggatgag tcggaccatc
    46441 atttccagtt ctgcatcggc cggtttaccg tgattcatca gccagttaaa caggtccgga
    46501 tcgtcacatt ccagcagacg aataaagatg cgtttttcgt catcgcttaa gctgtcgtac
    46561 tcatgttcga aaaacggcat gattgaaata tcgagttcgc gcataccacg gcggcatgcc
    46621 caatgaatgc gggctttgtt gttaatgtcc atcttcttcc tgtctcacga aaatccagta
    46681 cccggctatt gtaacgtgtt tttcgccttc ttttacggga atatcagtaa acaccatcgc
    46741 gatcgcgaaa ttaattcaca acaattcaat ggcttcattt ttttggaagt cgcctcgcag
    46801 aaggcacaga tcgcgtagtg aaagcacttg cattgcctca tagctctttt accattagtc
    46861 attaatacgc cgttaagcaa ctcaggactc tattatggct tttacacctt ttcctccccg
    46921 tcagcctacg gcttctgccc gtttaccgct gacgctgatg acgcttgatg actgggcgct
    46981 tgccaccatt actggcgcgg acagcgaaaa atatatgcag ggtcaggtga cagcagatgt
    47041 cagccagatg gcagaagatc agcacctgct cgccgcccat tgcgacgcca aaggtaaaat
    47101 gtggagcaat ttacgtctgt tccgcgacgg cgatggcttt gcatggattg aacggcgcag
    47161 cgtgcgtgaa ccgcagctga ctgaactgaa aaaatatgcg gtattctcta aagtgaccat
    47221 cgcgccagac gacgagcgtg tgctgcttgg tgttgccggt tttcaggcgc gcgccgcgct
    47281 ggcaaatctc tttagcgaac tgccttcgaa agaaaaacag gtagtcaaag aaggcgcgac
    47341 cactttgcta tggtttgaac acccggcaga acgtttcctg atcgtaaccg atgaagctac
    47401 tgctaatatg ctgaccgata aactgcgcgg tgaagcggaa ctgaacaata gccaacagtg
    47461 gctggcatta aacattgaag cgggtttccc ggtgattgat gccgccaaca gcgggcagtt
    47521 tatcccacag gcgaccaatc tccaggcgct gggcggtatc agctttaaga aaggctgtta
    47581 taccggacaa gagatggtgg cgcgagcaaa attccgtggt gccaataaac gtgcgctctg
    47641 gttgctggca ggtagcgcca gccgactgcc ggaagctggt gaagacttag agctgaaaat
    47701 gggcgagaac tggcgtcgta ccggtacggt gctggctgcg gtaaaactgg aagatggtca
    47761 ggtcgtggta caggtcgtca tgaataacga tatggaaccg gacagcatct tccgcgtacg
    47821 cgacgatgcg aatacactgc atatcgagcc gctgccgtat tcgctcgaag agtaaatctc
    47881 tgtatcgcat caggcattta tcgcctgatg cgacgctgac gcgtcttatc atgtctggga
    47941 tttgatgcct ttatcacaac agggacgagg cccgtagatc ggataaggcg ttcacgccgc
    48001 atccggcggt tgcgctccga tgcctgatgc gacgctggcg cgtcttatca ggcctacaaa
    48061 ggcataccca ttacgcctgc ccaatataca aatagatcgc cagaaagtgg cacacactac
    48121 cgccgagcac gaagccgtgc cagatggcat ggttgtatgg aatgcgtttg cagacgtaga
    48181 aaatcacccc gagcgaataa accacgccgc ctaccgccag taaggtaacg ctgcccgccg
    48241 cgagcttaac tgccatttca taaattacca ccagcgacag ccagcccatc gccagatagg
    48301 tcaccagaga taaaattttg aatcggtgcg cgatggtcag tttaaacaga atacccagca
    48361 atgccaggct ccagataaca atcatcaacc cgcgcgctaa cggagaatcc agccccacca
    48421 gcaaaaacgg cgtgtaggtt ccggcaatca acaggtaaat agcgcaatgg tcaaatttct
    48481 tcagccacat ttttgcccgt tgatgaggaa tggcgtgata gagcgtcgaa gcgaggaaca
    48541 gcaggatcat actgccgcca tagaggctgt aactggttat cgctgtggcg ctggcattga
    48601 gatccaccgc ctgaaccagt agcaacacca gcccaacgat accaaacacc aacccaatgc
    48661 cgtgactgac gctgttggca atttcctctg ccagcgaata tccctgctta atgaggggct
    48721 tctgaaccat aacttactcc ggagaaacgt acacgcacat gtatacctct ccagcgtaac
    48781 tgagaatagt tccagtgaac acctgttagc taaaataaat tctgatttga gactaatctc
    48841 ctaaaaatca tgaaattaaa tgcgaaattt caactaacag gcgtgagttc aatttaaaga
    48901 catttaaatt caatcacata aaactgtgtc tgaccgggat agatgtcggc aatgaccttt
    48961 atcagttcag tcagggtcat attttcctgc tctgcatgtt tttccgtcag cgtatccagc
    49021 gttacggttg aggttgcggt gacttcaatc gtgcaaaaat aaccgtcatc ttcaaaacgc
    49081 ccgacacgaa gcacatcacc cgttttgaag tgcgattcag actcgtcgcg gatggtgatg
    49141 gttttacgcc cagccagaat gtcatcctgg aaacgttgaa aaaaagtgat gtcgtttggc
    49201 tgcatggtac tatttcctgt aagaattgac tcatctggag cctatgatag tgaaaaaact
    49261 caccttaccg aaagatttct tatggggcgg cgcagttgcc gctcatcagg tcgaaggcgg
    49321 ctggaacaaa ggcggcaaag ggccgagcat ttgtgacgtt ctgaccggtg gcgcacacgg
    49381 cgtgccgcgc gaaatcacca aagaagtctt gccaggcaaa tactatccaa accatgaagc
    49441 cgttgatttt tatggtcact acaaggaaga catcaagcta tttgccgaaa tgggcttcaa
    49501 atgttttcgg acttccatcg cctggacgcg tatttttcca aaaggtgatg aagctcagcc
    49561 aaacgaagaa gggctgaagt tctacgatga tatgttcgat gaactgctga aatacaacat
    49621 cgaaccggtg atcaccctct cccactttga aatgccgctg catctggtgc agcaatacgg
    49681 tagctggacc aaccgtaaag tggttgattt ctttgtccgt ttcgcggaag tggtgtttga
    49741 acgctataag cataaagtca aatactggat gaccttcaac gaaattaaca accagcgtaa
    49801 ctggcgtgca ccgctgttcg gttactgctg ctccggcgtg gtttataccg agcatgacaa
    49861 cccggaagag acgatgtacc aggtgctgca tcaccagttt gtcgccagcg ccctggcggt
    49921 gaaagctgcg cgtcgcatta acccggagat gaaagtcggc tgtatgctgg cgatggtgcc
    49981 gctctatccc tactgctgta acccggacga tgtgatgttc gctcaggagt cgatgcgcga
    50041 acgctacgtc tttaccgatg tgcagctacg cggctattac ccgtcctatg tgttgaacga
    50101 gtgggagcgt cgcggattta acatcaaaat ggaagacggc gatctcgatg tactgcgtga
    50161 aggcacctgc gattatcttg gtttcagcta ttacatgacc aacgcagtga aggccgaagg
    50221 cggcaccggc gatgcgatct ctggtttcga aggcagcgta ccgaacccgt atgttaaagc
    50281 gtctgactgg ggctggcaga ttgatccagt aggtctgcgc tatgcccttt gcgaactgta
    50341 tgagcgttat cagaggccgc tgtttattgt cgaaaacggt tttggcgctt acgacaaagt
    50401 ggaagaagat ggcagcatca acgacgacta ccgcattgac tacctgcgcg cccatatcga
    50461 agagatgaaa aaagcggtga cttacgatgg cgtggatctg atgggctaca caccgtgggg
    50521 ctgcatcgac tgcgtatcgt tcaccaccgg gcagtacagc aaacgctacg gctttatcta
    50581 tgtgaataaa catgacgacg gtgctggcga tatgtcgcgt tcacgtaaga agagctttaa
    50641 ctggtaccaa gaggtgattg ccagcaacgg cgagaagctt taagtcgatg aagtaccgga
    50701 tgcaatactt gttgcatccg gtcagacaac ttccctgttt tatttcccgc ccgccaaatc
    50761 gataaaactt cccgtgacgt aagaggcttt atcacttagt agccagacaa tggcctgcgc
    50821 gacctcttct gcctgtccac cacgctgcat ggggatgttc gacttaacgc gatcgacgcg
    50881 tccaggctcg ccgccgctgg cgtgcatttc ggtataaata aaccctggcc gcacgcagtt
    50941 aacgcggatc ccctgcgcgg cgacttccag cgatagtccg gtggttaacg tatcaatcgc
    51001 ccctttcgat gccgcgtaat caacatattc ccctggcgaa cccaaccgtg aggccaccga
    51061 agagacattg acgatagcgc cgccactgcc accattttta agcgccatgc gttttaccgc
    51121 ctcgcggcag cagagaaaat atcccgtcac gttggtggaa agtactcggt tgattcgctc
    51181 tgcggtaagg ttttcaacgg tgcactgggt aaacaagatc ccggcgttat tgaccagcgc
    51241 tgctagcggt tcatcgtgct gatcgattgc tgtaaacatc gcaacgacct ggttttcgtc
    51301 gctgatatcc gcctggagca cgaatgcttt gccaccggct tgcgttatta agttcatcac
    51361 ttcctgcgcc gcgtggaggt tttgctgata attaaccgcc accgtatacc cttcttgcgc
    51421 caacagtaat gcagttgccc gcccgatgcc gcgactgcca ccggtcacaa gtgctatagc
    51481 catttgtttt tccgagaaaa attaattcgt tgacgtatct ttgagattgc taacaaactg
    51541 gctcaatctt caatgttggc acgcttttcc cctcgccctt tcagggagag ggccggggtg
    51601 agggtaaata ttcgcgccag tgccggcctg ttcccctcac cctaaccctc tccccaaagg
    51661 ggtgagggga ctgtctgagc gcttttgtac tttgtcatct gactaaaaag gcgccgaagc
    51721 gcctttagaa gatagtcgaa tcagtgaatt actggtattc gctaatcggt acgcaggagc
    51781 agaacaggtt acggtcgccg taaacatcat ccagacgttt caccgtcggc cagtatttgt
    51841 ctgccacacc tgccgggaat actgcaactt cacggctgta cggatgcgcc cactcggcga
    51901 ccagttcgtt ctgaatgtgc ggcgcgttca ccagcgggtt atcttccagc ggccagacac
    51961 cggctttaac ctggtcgatt tctgcgcgga tagccagcat cgcgtcgata aagcgatcca
    52021 gttccacttt gctttcagat tcagtcggtt caaccatcag cgtacccgcc accgggaacg
    52081 acatggtcgg cgcgtggaaa ccgtagtcga tcaggcgctt ggcaatatcc agctcgctga
    52141 tgccggtttc ttctttcagc gggcgaatat cgagaataca ttcgtgcgcc acgcgaccgt
    52201 cgcgaccggt atacagcacc gggaaggcat cctgcaggcg gctggcaata tagttggcgt
    52261 tgaggattgc cacctggctt gcttttttca gcccttctgt gcccatcatg cggatgtaca
    52321 tccagctgat tggcaggata gaggcgctac cgaacggtgc cgcagaaact gcgccctgac
    52381 gggttaacat gccttcgatt tgcaccacgc tatgacccgg tacaaacggt gccagatgcg
    52441 ctttcacacc gatcggtccc atacccggac caccaccgcc gtgcggaatg cagaaagttt
    52501 tatgcaggtt gaggtgcgaa acgtccgcgc caataaagcc cggcgaggtg atgccaacct
    52561 gggcgttcat gttcgcgcca tcaaggtaaa cctgaccgcc gaactgatgc acgacttcac
    52621 acacttcacg gatcgtttct tcatacacgc cgtgggtaga aggataagtc accatgatac
    52681 aggagaggtt atcgcccgcc tgttccgctt tcgcgcgcag atcagtcaga tcgatgttgc
    52741 cgtttttatc acacgccaca accaccacct gcattcctgc catatgtgca gaagcggggt
    52801 tagttccgtg cgcagaagcc gggatcaggc agatatcgcg atgcccttcg ttgcggcttt
    52861 catgataatg acgaatcgcc agcaggcccg cgtattcgcc ctgtgcgccg gagttcggct
    52921 gcatacaaac ggcgtcgtaa ccggtcagtt tcaccagcca gtcagccagc tgcgcaatca
    52981 tctgctgata accttcagcc tgttccggcg gacaaaacgg gtgcagttcg gcaaattccg
    53041 gccaggtgat tgggatcatc tcggcggcgg cgttcagttt catggtgcag gaacccagcg
    53101 ggatcatcgc ctgattcagc gccagatctt tacgctccag cgagtgcata tagcgcatca
    53161 tttcggtttc gctgtggtag cgattaaaca ccggatgggt gaggatttcg tcgtcgcgca
    53221 gcatcgcagg ctggatagag cggctgtcgt gagccacgtc tttgtccagc gtgtcgatgt
    53281 ccaggccgtg gttatcgccc agcagcacgt tgaaaagctg cattacgttt tcacgcgtgg
    53341 ttgtttcatc aagggtgatc ccaaccgcgt tcagaatatc gctacgcagg ttgatttcag
    53401 ccgcttcggc acgcgtcagt acgcccgctt tgtcggccac ttccacacac aaggtgtcga
    53461 aatagtgcgc atggcgcagt ttcagacctt tttgttgcag gcccgccgcc aggatatcgg
    53521 tcagacggtg aatgcggtta gcgatacgtt tcaggccaat cgggccgtga taaacggcat
    53581 acaggctggc gatgtttgcc agcagcacct gggaagtaca aatgttggag ttcgctttct
    53641 cacggcgaat atgttgctcg cgagtctgca tcgccatgcg cagcgcggta ttgccagctg
    53701 catctttcga tacaccgata atacggcccg gcattgagcg tttgtattca tctttcgccg
    53761 caaagaaggc cgcgtgtggg ccaccgtagc ccatcggcac gccgaagcgt tgcgccgaac
    53821 caaaaacaat atccgcgccc tgtttacccg gcgcagttaa cagcaccagc gccataatat
    53881 cggcggcgac actgaccaca attttgcgtg atttcagttc gctaataagc gcggtgtagt
    53941 cgtggatttc accggttgtg ccaacctgct gtaacagcac gccgaagacg tcctgatggt
    54001 cgagcacttt ttgcgcgtca tcgacaatca cttcaaaacc aaaggtttcg gcacgagtac
    54061 ggaccacatc cagcgtttgc ggatgcacat cggaagccac gaagaagcgg ttggcatttt
    54121 tcagtttgct gacgcgtttc gccatcgcca ttgcttcggc ggcagcggtg gcctcgtcca
    54181 gaagagaagc agaggccata tccagtccag tcaaatccag cgttacctgc tggaagttga
    54241 gcagtgcttc aaggcggccc tgggagactt caggttgata cggagtgtac gcggtatacc
    54301 agcccggatt ttccagcatg ttacgcagga taaccggcgg tagctgcacg gcggtgtaac
    54361 ccatgccgat gtaagacgtg aagcgtttat tgcgactggc aatagccttg agttctgcca
    54421 gtgcggcgta ttcggtcgcc ggtgcgccaa cctgcggcgg ggtcgcaagc tgaatatctt
    54481 tcggcacaat ctggccggtc agcgcgttta acgattgtgc accaacggca ttcagcattt
    54541 cttgctgttg cgcggcgtcc ggtccgatat ggcgttcaat aaaagcgccg ctgttttcaa
    54601 gctggcttaa cgtctgtgtc atgagcgatg gttcctgaaa cgtgcagtga attgtgaacc
    54661 tctctcctta cgaagagagt gagggtgagg cataaatttt cctcaccctg atcctctccc
    54721 gcagaagagg aataaagccg ttactcgtct tctaacaatg cttcgtatgc ggtcgcatcc
    54781 agcagtgatt ccagttcgct ttcatcgctg gctttgattt taaagatcca gccacctgca
    54841 tacggttcgc tgttcaccag ttccggggaa tcgctcagtg cgtcgtttac cgccacgatt
    54901 tcaccgctta ctggcgcata aatgtctgac gccgctttta ctgattcggc aaccgcgcag
    54961 tcatcgcccg cgctaaccgt tgcgcccact tccggcaggt caacaaacac catatcgcct
    55021 aacagctcct gggcatgttc ggttatacca acggtgtaag tgccgtcggc ttctttacgc
    55081 agccattcgt gttctttgct gtatttcagt tctgctggta cgttgctcat caatcaatct
    55141 ccaaaaaagt aaatcacgcg acggctttac cgttacgcac aaaaacaggt ttcgtcactt
    55201 taaccggcat ttcacggttg cgaatttgca caatcgccgt ttcgccaata ccttccggca
    55261 cgcgtgccag cgcaatgctg taacccagcg tcggggagaa agtcccgctg gtgataatgc
    55321 cttcatgctg gttgccctgc gcatcggtaa aacgtaccgg cagttcatta cgcagcacgc
    55381 ctttttcggt catcaccaga ccaaccagtt tttctgtgcc atgctcacgc tgcacttcca
    55441 gggcttcacg accgatgaag tcacgatctg ccggttccca ggcgatagtc cagcccatgt
    55501 tggcggctaa aggagaaata gtttcgtcca tctcctgacc ataaagattc atgcccgctt
    55561 ccagacgcag cgtgtcacgc gcgcccagac cgcatggctt aacgcccgct tccaccagcg
    55621 cacgccagaa atcggccgct ttttcattgg gcagcgcaat ttcatagccc gcttcaccgg
    55681 tataaccagt ggtggcaata aacagatcgc ccgcctgcac gccaaagaac ggtttcatcc
    55741 cttccaccgc ctgacgctga gcatcattaa acagtgtggc agcttttgcc tgcgcattcg
    55801 gcccttgtac ggcgatcatg gaaaggtcat cacgaacggt aatttcgatg ccgaaaggtt
    55861 cagcgtgttg ggtaatccag gagaggtctt tttcgcgggt ggcggagtta acaacgaggc
    55921 ggaagaaatc ttcagtaaag tagtagacga tgaggtcatc tatcacaccg ccagaggcat
    55981 tcaacatccc cgagtaaagg gctttgccgc ttttggtgag cttcgccaca tcgttcgcca
    56041 gcagataacg cagaaactcc cgggtgcggc tgccgcgaag atcgacgatg gtcatatgtg
    56101 acacatcaaa cattccggca tcggtacgta ccgcatgatg ttcgtcgatt tgcgaaccgt
    56161 aatgcagcgg catcatccag ccgtggaaat ccaccatgcg agcgccgcaa agcgtgtgtt
    56221 gttcgtacaa aggagtctgt tgtgccatct tgtcctcatt gaataagcgg ggctgacaac
    56281 tttttcatgg tgaaattatc accacgaaac ccagcatcgg agccactccc ggtccccaac
    56341 gcaatcgttc tcttttgcct gaacttacca ccgaaacaga ctgttaacca taaggtaaaa
    56401 ttgatcatca cattagctta tggttaaaaa acgccaaaat cgcaacagaa taaaaaacca
    56461 aaaaatacac aagtttttat ataaagatga tgtgatgaga aagttaattt gaataaggca
    56521 atattaagag ctgaaaaaat gtcaaaaaac actaaatcaa aaattcgtga cattagaaaa
    56581 tataatgcga aaacggaggt gaaattagtt tatttcaaat gaggaaaatc tcccggcgaa
    56641 aaaaccggga gatgaaagtg tgatgggtat caaataaaca gcagaagaga aatttttaac
    56701 gcagccattc aggcaaatcg tttaatccca ttgcctggcg gataagttgt ggtttaacgc
    56761 caggaagcgt gtcggccagt ttcaaaccaa tatcacgcag cagttttttc gccggattgg
    56821 taccggaaaa cagatcgcgg aatccctgca taccagccag catcagcgcc gcactgtgct
    56881 tgcggctacg ctcataccgg cgcaggtaaa tgtactgccc gatgtctttc ccctgacgat
    56941 gcaaccgttt cagttcagca atcagctctg cagcatccat aaagccgaga tttactccct
    57001 gccccgccag cgggtgaatg gtatgcgcgg cgtcgcccac cagcaccaga cggtgcgcgg
    57061 caaactggcg cgcataacgc cccgtcagtg ggaacacctg acgcgcgctc tcaaccttgc
    57121 ataagcccag gcgattatca aaagcgatat ttaacgcgcg attaaattca tcttcacttg
    57181 cctgctgcat ccgctgcgct tcctctggcg acagtgacca gacaattgag caaagatgcg
    57241 gatcgctaag cggtaaaaag gccagaatgc cttcgccatg gaaaacctgc cgcgccaccg
    57301 catcatgcgg ttcttccgtg cgaatggtcg ctaccagcgc gtgatgctga taatcccaga
    57361 aagtcagcgg aatatctgct ttgttgcgca accaggaatt agcaccgtcc gcgccaatca
    57421 ccagacgcgc cgttaacatg ctgccatctt tcagcgtcag gaaagtttca ttttctcccc
    57481 aggcgacctg ctgtaattct gcgggggcca acagagtgat atctgacgac tgctgcgctt
    57541 tgttccacag cgcgtagtga atcactgagt tttcaacgat atgcccaaga tggctatagc
    57601 ccatgctttg atcgtcaaac gaaatgtgac caaaactgtc tttgtcccac acttccatac
    57661 cgtgataaca actggcccta cgagagagaa tgtcctgcca gacgccaaga cgggtgagta
    57721 atttttcgct ggcggcattg atagccgaaa cgcgcagttg tggtggtgca tccgccgcca
    57781 gaggttccgg tacgcgctgc tccagtacgg caacgcgtaa gccgctcccc tgtaagccgc
    57841 aggcaaccgc cagccccacc atgccgccac caacaatggc tacatcaaca ctttgcattg
    57901 tttattcctt aaaaccgcct tcaacgcgcc acccaaccga gggtgcgctg cgccagcaca
    57961 tcgcgtgccg gggtgaataa ttccatcgtc atcagtccga tgttgcgccc gacaaccagc
    58021 ggtgcccaac ggttggcaaa aagatgtacc aggctgtccg tgacgccgat ggttgcttcg
    58081 cgatcgctct gtcgacgctg ttgatagcgg cacaatatgc cgtaatcacc gatgtcttct
    58141 ccgcgctcat gcgcctgagt tagggtttcc gcaaggctca tgacatctcg cataccgagg
    58201 ttaaaccctt gcccggcaat cgggtgcaga gtttgcgccg cattgcccac cagcacggta
    58261 cgatgggtaa tagctctggc ggcgcgggtt aacgccagcg gataagcact gcgtttaccg
    58321 gcgtgggtaa tttttccaag tcgccagcca aaggccgact ggagttcacg gcaaaacttc
    58381 tcgtcgctcc acgacagcac ctcttcgcgc cgttccagtg gatgacacca gaccagcgaa
    58441 cagcgtccgt cagacatcgg caacatcgcc agcgggccat gttgcgtgaa gcgttcaaaa
    58501 gcgcggcctt cgtgcgcaac ggaagtcgca acgttggcaa tcacggccag ttgttcgtaa
    58561 ggctcctgct gccagtcaac gccgcacgcg gtggctaacg ctgaatgggt gccatcagct
    58621 gctaccagca cgcggcccgt cagcgtctcg ccactctcca gcgtcacttc aacgtgactc
    58681 tgagtacggg caacgttagc cacgcgatca gggcaatgca gcgttacgcc aggtgcttta
    58741 cgcagcaatg caaacagccg ttgcccgaca ttgtgcaatt cgacaacctg tcccagcgcc
    58801 gccagttggt aatcttctgc ggcgagggtg acaaatccag cgtgaccacg atcgctgaca
    58861 tgcacggtag tgatggcggt tgcgtaatcc gccagagatt gccagacgcc gatgcgcgcc
    58921 agttgctgac aggtccccgc agccagcgca atagctcgtc catcaaagcc cggatgagca
    58981 tgtgactctg gcgcggtcgc ttcaatcaaa tgtaccggca acgccccgtg acttaaccgg
    59041 gaaatagcca gcgccagcgt cgcgcccgcc atgccgccac cgacgatgat tacgctcatt
    59101 gctttctcgc agctgccatc aacgcttcga tttcttccgg ctttttcacc acgctggcgg
    59161 tgaggttttc gttgccggtt tcggtaatca caatgtcgtc ttcaatacga atgccgatac
    59221 cgcgatattg ttctggcaca tccgcatccg gcgcgatata cagaccaggc tctacggtca
    59281 gtaccatccc cggttccaga atgcgcgagc gatcctgacc ataaacgcca acgtcatgga
    59341 catccagtcc taaccagtgg ctaaggccat gcataaagaa aggacgatgg gcgttctgag
    59401 cgatcagttc atcaatatca cctttcagga tgccgagttt taccaggccg ctaaccataa
    59461 tgcgcaccac ttcaccggtg acttccagaa tggaggttcc cggacgatac aggcgcaggc
    59521 tggtttcaag agactccagc acaatgtcgt agatttcacg ctgggcctga gtgaatttgc
    59581 cgttgaccgg gaaggtgcgg gtaatatcgc cagcgtaacc tttgtattcg caacccgcat
    59641 caatcaacac caggtcaccg tcacgcagtt cacactcgtt ttcggtgtag tgcagaatgc
    59701 agccgttttc accgctgccg acaatggtgt tataggaagg atagcgcgcg ccgtgacggt
    59761 taaattcgtg gtgaatttcg ccttccaggt ggtactcgaa cattcccgga cggcattttt
    59821 ccatcgcccg ggtatgcgcc agggcggtga tttcacccgc gcggcgaagt acggcaatct
    59881 cttccggcga tttgaacagg cgcatttcat gaacaatggg acgccagtcg atcatcgttg
    59941 ctggcgcggt gagattttgt cgcgaacctt tacgcagctt ttccagcgcg ctgttcacga
    60001 tttcatcagc ataggtatat tcgccctggg cgtggtaaac cacatccaga ccattaagta
    60061 attgataaag ttgctgatta atttcgctga atgccagtgc gcggtcaacg cccagtttct
    60121 ctggcgcggc atcctgacct aaacgacggc caaaccagat ctccgccgtc aggtcgcgaa
    60181 cgcggttaaa cagaacgctg tggttatgag tgtcatcgct tttaatcagc accagcaccg
    60241 cttccggttc gttaaagccg gtgaagtacc agaagtcact gttctgacga taggggtatt
    60301 cgctgtcggc gctacgtgtt acttctggtg cagcaaaaat cagcgcggcg ctgccgggtt
    60361 gcatttgctc caccagggcc tgacggcgac gctgaaactc ttgccgggat atatcactca
    60421 taacactctc cttacgtttt ttgtttttag tgtagagtcg gtttttgtac ttctggcgcg
    60481 gtcggttgcg gatgagtaaa ggtgtcgtgg cataacagcg cggcaacacg aacgtattcg
    60541 atgatctctt caagcgacat ttcaagctct tcctgatctt cgtcttcgtc gtaacccagt
    60601 tgcgcaatgt tacgcagatc gtcgatagct tcaccggttt cgccggtcac tttatccagc
    60661 ttcggttgcg taacgccaag accaagcagg aagtgattga cccaacctgc cagtgcatca
    60721 gcccgatcga aaacgctgac atcatcgcca tcaggcagat aaagctgaaa aaggaagccg
    60781 tcatcctgca gggcatcgct ggtggcagag tgcattttac gcagtgcctg tgccagctca
    60841 tgaccgaaag ccatgccttc gttcgtcagg tcgtgaagta gcggtagcca tgagctgtca
    60901 tcgttaccgc cacatatcat cccactgatt aaaccatgca tctcagcagg ggtcagaccc
    60961 gtcccttgtt ggttcagata ctggttcatt tcgttgtaac caggcatttc gttctgtata
    61021 gacataagca ttcgtcgtca aagggaggaa tattcatgat atgctaccac tttgggccct
    61081 ggtggaccag aaaagggctt gtctcttctc atcagggtag ctatagtgtc gccccttcgc
    61141 agaccatggg tctaaagacg aaggcagcgc agtcaatcag caggaaggtg gcatgtctgc
    61201 acaacccgtc gatatccaaa tttttggccg ttcactgcgt gtgaactgcc cgcctgacca
    61261 aagggatgcg ttgaatcagg cagcggacga tctgaaccaa cggttgcaag atctgaaaga
    61321 acgcactaga gtcacaaata ctgaacagtt ggtcttcatt gccgcattga atatcagcta
    61381 tgagttagcg caagaaaaag cgaagactcg tgactacgcg gcaagtatgg aacagcgtat
    61441 tcggatgctg cagcagacca tagaacaagc gttacttgaa caaggtcgca tcaccgaaaa
    61501 aactaaccaa aactttgaat gacacttttc ggtttactgt ggtagagtaa ccgtgaagac
    61561 aaaatttctc tgagatgttc gcaagcgggc cagtcccctg agccgatatt tcataccaca
    61621 agaatgtggc gctccgcggt tggtgagcat gctcggtccg tccgagaagc cttaaaactg
    61681 cgacgacaca ttcaccttga accaagggtt caagggttac agcctgcggc ggcatctcgg
    61741 agattccctt cttatctggc accagccatg acgcaactac cagaactccc actgacatta
    61801 tcccgacaag aaatccgcaa aatgattcgg caacgtcgtc gtgcgttaac gccggaacaa
    61861 cagcaggaaa tgggtcaaca agccgctacc cggatgatga cttatccccc ggtggtgatg
    61921 gcgcatacgg tcgctgtatt cctctctttt gatggtgaac tcgacaccca gccgctaata
    61981 gaacaactct ggcgcgccgg taagcgcgta tatcttccag ttttgcatcc ctttagtgcc
    62041 ggtaatttgc tgttcctgaa ttaccatccg caaagcgaac tggtgatgaa caggttgaag
    62101 atccatgagc caaaactgga tgtgcgtgac gtgctacccc tttcccgatt agacgtgctg
    62161 atcacaccgc tggtcgcctt tgatgagtac ggtcagcgcc tgggaatggg cggtggtttt
    62221 tatgatcgga ccttacaaaa ctggcagcac tataaaacgc aaccggtggg ttatgcgcat
    62281 gattgtcagt tggtggaaaa actccccgtt gaagagtggg atatccctct tcctgcggtg
    62341 gttacaccgt cgaaagtctg ggagtggtaa gggcgataca cctgcatcgc cctgattgac
    62401 atcgttgatt ctttaaccta atttagtgag taagggtaag ggaggattgc tcctcccctg
    62461 tgactgactg ttaataagct gggaaactta tgagtaacag tacaatcagt atgatggcaa
    62521 gtcgcatcat aacccttctc cttcaagccc tcgcttcggt gagggcttta ccgttacagc
    62581 cccatgctgc ccagctatcg taagctctct ttaaataacc acaacgcatt gatctacctt
    62641 taaatctttt cttcgagcgt gctcgaaaaa ccccatgcaa aacagagata atttttggga
    62701 aaaaggatct attacggcag taaacgtggg cgatgaagtg gctatcaaaa agttcggttc
    62761 agggcgatgc accccgcatc gccctgattg acatcgttga ttctttgacc taatttagtg
    62821 agtaagggta agggaggatg actcctcccc tgagactgac tgttaataag ctccgaaact
    62881 tatcagtaac agtacaatca gtatgatgac aagtcgcatc ataacccttc tcctttaagc
    62941 cctcgcttcg gtgagggctt taccgttaca gccccatgct gtcccgccat cgtaaatccc
    63001 cattaaataa acacaacgca ttgatctgac tttgatttat tttctggagc agactcgcaa
    63061 agtagaatgc gcaacgcggc aacggtgtgg agaagggata aaaaaacggg caagtcagtg
    63121 acctgcccgt taattttcag agaaggggaa ttagtacagc agacgggcgc gaatggtacc
    63181 cggaatagct ttcattgcct gcagcgcttt ttcggcaacg tcttcgtcag cttcaatatc
    63241 aataactaca taacccatct gggcggaagt ttgtagatat tgcgcggcga tgttgacgcc
    63301 ctgctcggcg aagattttgt tcagcgcagt tagcacgccc ggacggtttt cgtggatgtg
    63361 catcagacga cgaccaccat gcagtggcag cgagacttcc gggaagttca ccgcagagag
    63421 cgttgagcca ttgtcagaat acttgatcaa tttacccgca acttccaggc cgatattctc
    63481 ctgcgcttcc tgagtcgaac cgccaatgtg tggcgtcaga aggacgttgt cgaattcaca
    63541 cagcggagag gtaaatggat cgctattggt cgccggttcc gtcgggaata cgtcgattgc
    63601 cgcccccgcc agatgtttgc tcgccagcgc atcacacagc gccggaatat ccaccacagt
    63661 accgcgcgaa gcattaatca gcagcgagcc gggcttcatt agtgaaattt ctttcgcgcc
    63721 catcatattt ttggtggacg gattctctgg tacatgcaga ctcaccacat cgctcatatt
    63781 cagcaggtca gaaagatgct gtacctgagt ggcgttgccc agcggcagtt tattttcaat
    63841 atcataaaag taaacataca ttcccagcga ttcagccaga atgcccaatt gcgtaccaat
    63901 atgaccgtag ccgatgatac ccagcttttt gccgcgcgct tcaaaagaac ccgccgccag
    63961 tttgttccac acgccacggt gcgctttagc attggcttcc ggcacgccgc gcaatagcag
    64021 cagcagttcg ccaatcacca gctccgcaac agagcgcgta tttgagaacg gtgcgttaaa
    64081 taccgggatc ccgcgctttg ccgccgcatc cagatcaacc tggtttgttc cgatacagaa
    64141 acagccaata gcgaccagtt tttctgcggc gttgatcacg tcttcagtca gatgggtacg
    64201 ggatcgcagg ccgatgaagt gggcatcgcg gatggattct tttaattgtt catcatccag
    64261 cgcgcctttg tgaaattcga tgttggtgta accagctgca cgaaggcttt ccagcgcctt
    64321 ttggtgcacg ccttctacca gcagaaactt aatcttgtct ttctccagcg atacctttgc
    64381 catttaccca atcctgtctt ttgaaatgtt gtgtgcggat ttgcgtccgc ctttcaacat
    64441 atcaaaaaat aatattgcgg caatatgaac gtttgcgtcg cgatgttgag gaaatatcac
    64501 gcaaaggtaa ttcttggatg aatatgctgg tttagaagat ttatttagag caatcgacaa
    64561 tagccaggta aaaacgtgac acatgtcacc aaatttaatg aagagaattt ttttaacggg
    64621 ggaggttccc ccgtcagatc atttcacaat ggttttgaca ccgtcaggtg tgccaatcag
    64681 cgcaacgtcc gcgccacggt tagcaaacaa gccaacagtc accacgccag gaatcgcatt
    64741 tatggcgttt tccatcgcta tcgggtcaag gatttccatg ccgtggacgt cgaggatcac
    64801 gttgccatta tcggtcacca cgccctgacg gtattccgga cgaccgccca gtttcaccag
    64861 ctggcgcgcc actgcactac gtgccatcgg gataacttct actggcagcg ggaatttacc
    64921 cagaatatca acctgcttgg aagcgtctgc aatacagata aatttttctg caaccgaagc
    64981 aatgattttt tcacgggtca gcgccgcgcc gccgcctttg atcatttgca tgtggccgtt
    65041 gatttcatct gcgccatcaa cgtagatgcc aaggctgtcg acttcgttga gatcaaaaac
    65101 gtgaatgccg aggcttttca gtttttcagt ggaagcatct gaactggaaa cggccccttc
    65161 aatctggcct ttcattgtac cgagcgcgtc aataaagtgt gcggcggtgg aacctgtacc
    65221 tacaccaaca atggtgccgg gctgaacata ctgaagtgcc gcccatccta ctgctttttt
    65281 caattcatcc tgcgtcatga tcgtttcgcc tgtggtatga aatttcacac gcattatata
    65341 caaaaaaagc gattcagacc ccgttggcaa gccgcgtggt taactcatcc ataaaatatc
    65401 gcgcaatggc aggcatcccc tttcgccccg caaataaggc atgcaacaat ctgggtatgc
    65461 cgctccacgg tgcaaacagg cgcaccagtt caccgttcgc aagcccctgt ttacaggcaa
    65521 attgaggcaa taacgccacg ccattcaaca caaccagggc gcgaactcgc tgagcgaaat
    65581 gcattagcgc acatccctga tcttttaagc tgcgcgttta accacgaata caaatgttac
    65641 ccgccgacgg cttcggtata tgcaacctga cacaaaattg tgtcatagtg caggaaaaag
    65701 catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat tacaggcact
    65761 ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt gcattacaca
    65821 atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc cgctgttggt
    65881 gcgtaccgta ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac tgctgcgcca
    65941 ggtggagttg ctggaagaag agtggctggg cgatgaacaa accggttcga ctccgctgct
    66001 gctttcactg gcggtcaacg ccgacagtct ggcgacgtgg ttgcttccgg cactggctcc
    66061 tgtgttggct gattcgccta tccgcctcaa cttgcaggta gaagatgaaa cccgcactca
    66121 ggaacgtctg cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc aggcgctgcc
    66181 gagttgtctt gtcgataagc tcggtgcgct cgactatctg ttcgtcagct caaaaccctt
    66241 tgccgaaaaa tatttcccta acggcgtaac gcgttcggca ttactgaaag cgccagtggt
    66301 cgcgtttgac catcttgacg atatgcacca ggcctttttg cagcaaaact tcgatctgcc
    66361 tccaggcagc gtaccctgcc atatcgttaa ttcttcagaa gcgttcgtac aacttgctcg
    66421 ccagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagagc tggccagcgg
    66481 tgaactgatt gacttaacgc ctgggctatt tcaacgacgg atgctctact ggcaccgctt
    66541 tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttactcgatt atggtcacaa
    66601 agtccttcgt caggattaat ccatcaaata atgcctgata gcacatatca ggcatttccc
    66661 tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaaatctca ggcggcaaaa
    66721 aacgacgtct gaatgcattt tttttgctgg cgacaaaccc acgtaaaaag ctcaccgtag
    66781 gcgcaaatac cctcattttg attgcgtttt acggagcaaa taatgtctaa cgtgcaggag
    66841 tggcaacagc ttgccaacaa ggaattgagc cgtcgggaga aaactgtcga ctcgctggtt
    66901 cagcaaaccg cggaagggat cgccatcaag ccgctgtata ccgaagccga tctcgataat
    66961 ctggaggtga caggtaccct tcctggtttg ccgccctacg ttcgtggccc gcgtgccact
    67021 atgtataccg cccaaccgtg gaccatccgt cagtatgctg gtttttcaac agcaaaagag
    67081 tccaacgctt tttatcgccg taacctggcc gccgggcaaa aaggtctttc cgttgcgttt
    67141 gaccttgcca cccaccgtgg ctacgactcc gataacccgc gcgtggcggg cgacgtcggc
    67201 aaagcgggcg tcgctatcga caccgtggaa gatatgaaag tcctgttcga ccagatcccg
    67261 ctggataaaa tgtcggtttc gatgaccatg aatggcgcag tgctaccagt actggcgttt
    67321 tatatcgtcg ccgcagaaga gcaaggtgtt acacctgata aactgaccgg caccattcag
    67381 aacgatatcc tcaaagagta cctctgccgc aacacctata tttacccacc aaaaccgtca
    67441 atgcgcatta tcgccgacat catcgcctgg tgttccggca acatgccgcg atttaatacc
    67501 atcagtatca gcggttacca catgggggaa gcgggtgcca actgcgtgca gcaggtagca
    67561 tttacgctcg ctgatgggat tgagtacatc aaagcagcaa tctctgccgg actgaaaatt
    67621 gatgacttcg ctcctcgcct gtcgttcttc ttcggtatcg gcatggatct gtttatgaac
    67681 gtcgccatgt tgcgtgcggc acgttattta tggagcgaag cggtcagtgg atttggcgca
    67741 caggatccga aatcactggc gctgcgtacc cactgccaga cctcaggctg gagcctgact
    67801 gaacaggatc cgtataacaa cgttatccgc accaccattg aagcactggc tgcgacgctg
    67861 ggcggtactc agtcactgca taccaacgcc tttgacgaag cgcttggttt gcctaccgat
    67921 ttctcagcac gcattgcccg caacacccag atcatcatcc aggaagaatc agaactctgc
    67981 cgcaccgtcg atccactggc cggatcctat tacattgagt cgctgaccga tcaaatcgtc
    68041 aaacaagcca gagctattat ccaacagatc gacgaagccg gtggcatggc gaaagcgatc
    68101 gaagcaggtc tgccaaaacg aatgatcgaa gaggcctcag cgcgcgaaca gtcgctgatc
    68161 gaccagggca agcgtgtcat cgttggtgtc aacaagtaca aactggatca cgaagacgaa
    68221 accgatgtac ttgagatcga caacgtgatg gtgcgtaacg agcaaattgc ttcgctggaa
    68281 cgcattcgcg ccacccgtga tgatgccgcc gtaaccgccg cgttgaacgc cctgactcac
    68341 gccgcacagc ataacgaaaa cctgctggct gccgctgtta atgccgctcg cgttcgcgcc
    68401 accctgggtg aaatttccga tgcgctggaa gtcgctttcg accgttatct ggtgccaagc
    68461 cagtgtgtta ccggcgtgat tgcgcaaagc tatcatcagt ctgagaaatc ggcctccgag
    68521 ttcgatgcca ttgttgcgca aacggagcag ttccttgccg acaatggtcg tcgcccgcgc
    68581 attctgatcg ctaagatggg ccaggatgga cacgatcgcg gcgcgaaagt gatcgccagc
    68641 gcctattccg atctcggttt cgacgtagat ttaagcccga tgttctctac acctgaagag
    68701 atcgcccgcc tggccgtaga aaacgacgtt cacgtagtgg gcgcatcctc actggctgcc
    68761 ggtcataaaa cgctgatccc ggaactggtc gaagcgctga aaaaatgggg acgcgaagat
    68821 atctgcgtgg tcgcgggtgg cgtcattccg ccgcaggatt acgccttcct gcaagagcgc
    68881 ggcgtggcgg cgatttatgg tccaggtaca cctatgctcg acagtgtgcg cgacgtactg
    68941 aatctgataa gccagcatca tgattaatga agccacgctg gcagaaagta ttcgccgctt
    69001 acgtcagggt gagcgtgcca cactcgccca ggccatgacg ctggtggaaa gccgtcaccc
    69061 gcgtcatcag gcactaagta cgcagctgct tgatgccatt atgccgtact gcggtaacgc
    69121 cctgcgactg ggcgttaccg gcacacccgg cgcggggaaa agtacctttc ttgaagcctt
    69181 tggcatgttg ttgattcgtg agggattaaa ggtcgcggtt attgcggtcg atcccagcag
    69241 cccggtcact ggcggtagca ttctcgggga taaaacccgc atgaatgacc tggcgcgtgc
    69301 cgaagcggcg tttattcgcc cggtaccatc ctccggtcat ctgggcggtg ccagtcagcg
    69361 agcgcgggaa ttaatgctgt tatgcgaagc ggcgggttat gacgtagtga ttgtcgaaac
    69421 ggttggcgtc gggcagtcgg aaacggaagt cgcccgcatg gtggactgtt ttatctcgtt
    69481 gcaaattgcc ggtggcggcg atgatctgca gggcattaaa aaagggctga tggaagtggc
    69541 tgatctgctc gttatcaaca aagacgatgg cgataaccat accaatgtcg ccattgcccg
    69601 gcatatgtac gagagtgccc tgcatattct gcgatgtaaa tacgacgaat ggcagccacg
    69661 ggttctgact tgtagcgcac tggaaaaacg tggaatcgat gagatctggc acgccatcat
    69721 cgacttcaaa accgcgctaa ctgccagtgg tcgtttacaa caagtgcggc aacaacaatc
    69781 ggtggaatgg ctgcgtaagc agaccgaaga agaagtactg aatcacctgt tcgcgaatga
    69841 agatttcgat cgctattacc gccagacgct tttagcggtc aaaaacaata cgctctcacc
    69901 gcgcaccggc ctgcggcagc tcagtgaatt tatccagacg caatattttg attaaaggaa
    69961 tttttatgtc ttatcagtat gttaacgttg tcactatcaa caaagtggcg gtcattgagt
    70021 ttaactatgg ccgcaaactt aatgccttaa gtaaagtctt tattgatgat cttatgcagg
    70081 cattaagcga tctcaaccgg ccggaaattc gctgtatcat tttgcgcgcg ccgagtggat
    70141 ccaaagtctt ctccgcaggc cacgatattc acgaactgcc gtctggcggt cgcgatccgc
    70201 tctcctatga tgatccattg cgtcaaatca cccgcatgat ccaaaaattc ccgaaaccga
    70261 tcatttcgat ggtggaaggt agtgtttggg gtggcgcatt tgaaatgatc atgagttccg
    70321 atctgattat cgccgccagt acctcaacct tctcaatgac gcctgtaaac ctcggcgtcc
    70381 cgtataacct ggtcggcatt cacaacctga cccgcgacgc gggcttccac attgtcaaag
    70441 aactgatttt taccgcttcg ccaatcaccg cccagcgcgc gttggctgtc ggcatcctca
    70501 accatgttgt ggaagtggaa gaactggaag atttcacctt acaaatggcg caccacatct
    70561 ctgagaaagc gccgttagcc attgctgtta tcaaagaaga gctgcgtgta ctgggcgaag
    70621 cgcacaccat gaactccgat gaatttgaac gtattcaggg gatgcgccgc gcggtttatg
    70681 acagcgaaga ttaccaggaa gggatgaacg ctttcctcga aaaacgtaaa cctaatttcg
    70741 ttggtcatta atccctgcga acgaaggagt aaaaatggaa actcagtgga caaggatgac
    70801 cgccaatgaa gcggcagaaa ttatccagca taacgacatg gtggcattta gcggctttac
    70861 cccggcgggt tcgccgaaag ccctacccac cgcgattgcc cgcagagcta acgaacagca
    70921 tgaggccaaa aagccgtatc aaattcgcct tctgacgggt gcgtcaatca gcgccgccgc
    70981 tgacgatgta ctttctgacg ccgatgctgt ttcctggcgt gcgccatatc aaacatcgtc
    71041 cggtttacgt aaaaagatca atcagggcgc ggtgagtttc gttgacctgc atttgagcga
    71101 agtggcgcaa atggtcaatt acggtttctt cggcgacatt gatgttgccg tcattgaagc
    71161 atcggcactg gcaccggatg gtcgagtctg gttaaccagc gggatcggta atgcgccgac
    71221 ctggctgctg cgagcgaaga aagtgatcat tgaactcaat aactatcacg atccgcgcgt
    71281 tgcagaactg gcggatattg tgatccctgg cgcgccaccg cggcgcaata gcgtgtcgat
    71341 cttccatgca atggatcgcg tcggtacccg ctatgtgcaa atcgatccga aaaagattgt
    71401 cgccgtcgtg gaaaccaact tgcccgacgc cggtaatatg ctggataagc aaaatcccat
    71461 gtgccagcag attgccgata acgtggtcac gttcttattg caggaaatgg cgcatgggcg
    71521 tattccgccg gaatttctgc cgctgcaaag tggcgtgggc aatatcaata atgcggtaat
    71581 ggcgcgtctg ggggaaaacc cggaaattcc tccgtttatg atgtattcgg aagtgctaca
    71641 ggaatcggtg gtgcatttac tggaaaccgg caaaatcagc ggggccagcg cctccagcct
    71701 gacaatctcg gccgattccc tgcgcaagat ttacgacaat atggattact ttgccagccg
    71761 cattgtgttg cgtccgcagg agatttccaa taacccggaa atcatccgtc gtctgggcgt
    71821 catcgctctg aacgtcggcc tggagtttga tatttacggg catgccaact caacgcacgt
    71881 agccggggtc gatctgatga acggcatcgg cggcagcggt gattttgaac gcaacgcgta
    71941 tctgtcgatc tttatggctc cgtcgattgc taaagaaggc aagatctcaa ccgtcgtgcc
    72001 aatgtgcagc catgttgatc acagcgaaca cagcgtcaaa gtgatcatca ccgaacaagg
    72061 gatcgccgat ctgcgcggtc tttccccgct tcaacgcgcc cgcactatca ttgataattg
    72121 tgcacatcct atgtatcggg attatctgca tcgctatctg gaaaatgcgc ccggcggaca
    72181 tattcaccac gatcttagcc acgtcttcga cttacaccgt aatttaattg caaccggctc
    72241 gatgctgggt taatggtttg tcccgtcacg atcttcagca atatgctgaa gcatcgtggc
    72301 ggtataacga tgatttctga ttgaataaag atattcctgc atatacatcg gcctgtgcgg
    72361 cgcatcaaaa tatttcagcg ttgccggatt caccagtcgc caggcaaaat gcgggatcac
    72421 cgtcagaaaa tgtccgcgct ccacggcgct gattttggcc ataaaactgt agggacgata
    72481 aataatggtt ggattgatcc cacaaggtcg catattcgca tcaatcattg cttcgaagtt
    72541 ggcccggttc tgaaagcgca tttgtagcca gggcagctcc tgaagcagct cttgctcttg
    72601 acgctcttca taacggcgag aaacgagaaa gcctaatcgc agcggtggta gttcactgat
    72661 agtcaggttt tccagttcct gaactcgtgc agatacatgc tgtggggaga taataaaatc
    72721 taactggcgg tcaaaaagat tatcgataac gccgttttcg ctgaactcaa ctggctgggc
    72781 ggtcacgcct tcatacttat ctccgagact aatcaactga tcgaaaataa ttgtcggata
    72841 agtattatct atgccaatca caatttctcg tgaacgacgt cctgagttat ggatttcatt
    72901 atcaatcgca gaaagccgtt gataaaccgg gaacagtttt tgatacagtt cctgtccggc
    72961 tttattgagg ctgatattat tatctttgcg ggtaaatagc gtatagccaa tttgttcttc
    73021 cagcgcggca atgcttttac caaatggcga agccgtcata tggatttttt ctgccgcccg
    73081 ggcaatatta ttggtttgcg ctaataaaat gaaattgcgc atctttttcg aaataaaaat
    73141 gtccatcccc ctcccccgca tccttgtccg cttaaccgtt tcagtcagcc tatccttgat
    73201 gaaaccgcga gcaaagatag gtgattacgt catggtttta cagaaaatta cagaaaaagg
    73261 aggcaatatc gggtaaaggc attagcccga cgaatacgtc gggctacaag tattattgtg
    73321 ctgcaggtgt tttagcgggt tgttgatcca caggttctaa ctggaagacc acatcgacct
    73381 gatcatcaaa ctgaatagcg gcctgctcgt aagtttcctg ggcggacacc ggcgcggcat
    73441 cggctttcat catccgcacc attgggctgg gctgatagtt ggaaacatgg tagcgcacgc
    73501 tatataccgg ccccagttta cgatgaaagc cgttcgccag ttcctgcgcc tgatgaatcg
    73561 cgttatcaat cgctgcctta cgcgctttgt ctttataggc atccggctgc gccacgccca
    73621 gcgacacaga acgaatttcg ttaagacccg ccttcagcgc gccatccagc aaggaattca
    73681 gtttgtctaa ctgacggagc gtgacttcca ccgttctcac agcgcggtag cctttcagga
    73741 tacttttacc atcctgataa tcataatctg gctgggtgcg taagttcgct gagctgatat
    73801 cttttttcgc gatctgattg agttcaagga aggaaatgta ttgtgcgacg cgctcatctg
    73861 cctgtttctt ggcagtagcg gcatccttcg cggccacgtt aacttcaatc gcaagagtgg
    73921 caatgtctgg caccgcatcc acgcttgccg taccggaggt gacaatatgc ggtccatccg
    73981 gcaattcgtt agcctgcgct gccatcccgc taatacccat taatgccgcc agggcgataa
    74041 ctttgaactt cacgttagtc tctccgtgtt aattgacttg cccaaaattc aggacctctg
    74101 gaggcaagct tagcgcctct gttttatttt tccatcagat agcgcctaac tgaacaaggc
    74161 ttgtgcatga gcaataccgt ctctcgccag ctgcaaggct ataaaccaca tgacacatcc
    74221 cactaccaga ttgataatgc gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc
    74281 tgcgagaata gccagaccaa agaaccacag gaaagaggcg ctaattgtcc cgagcgcaaa
    74341 ccaacgtttt ggttcgacgt caagctgccc gccaaggctg cccagtacaa caaaagtatc
    74401 caggtaaaca tgcggattca gccaggtcac tgccaacatg gtggcgataa ttttccatct
    74461 gccttgcttc aggacttcgg cactggctaa ctcaatatta ctgctcattg cggttttaaa
    74521 agcgccaaaa ccataccaca gcaagaaggc tacgccgccc caggtgacca gcgccagcaa
    74581 ccacggcgac tgcatcaata acgcgctgcc accaaaaatc ccggcgcaaa tcaggaccaa
    74641 atcgctgata gcacaaagta aggcaatcat aatgtggtac tgacgacgaa tgccctgatt
    74701 catcacaaaa gcattttgtg gaccgagcgg taggatcata gctgcgccaa gcgcaagacc
    74761 ttgaaagtaa taagaaaaca cgttggatat tccgaattaa gtatctgatg cagagactat
    74821 atcgcgggat tgttattagc ggaaattgat aatattaatt ggcaatcaga aaaactaata
    74881 agagagaggc cagagagtat tatctggcct cagcgttgat tacgcagctt tgtcttcttt
    74941 cacccgctta aagttcacat ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa
    75001 ttcacgttta atacgctcca gcacatccca gtacacgttt tgcagatcgc cgctgttgct
    75061 ccagacgcgg accacgaaat taatcgacga tgcaccaagt tcgttcaggc gcacagtcat
    75121 ttcgcgatct ttcaaaatgc gatcttcaga ctggataata ttggtcagga tctgcttaac
    75181 ctgatcgata tcggaatcat acgccacgcc aataataaat tcgttacggc gaactggctc
    75241 gcgggagaag ttaataatat ttccggcaat aattttaccg ttcggaataa cgataatttt
    75301 accgtctgca gtacgcatgg tggtggagaa aatctgcaca ctcagcacag taccggctac
    75361 gccgccgagg tcaacatatt ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc
    75421 agcggccagg ttagacagtg acccctgcaa agccagacca acagctaagc ctgcggcacc
    75481 gagtacagca atgacggacg cggtttgtac acccacgcgt cccagtgcag cgattagagt
    75541 aaaggcgata ataccgtaac ggactaatgc agaaagaaaa tcagcaacag tggcatcgat
    75601 tttacgggag atcatcaggc gattcaccgc gttggaaatc atccgcgcga taatcaaacc
    75661 aacgatgatg atcgcgagtg ccgccacgat gtttactgca taacttagca gcagcgcctg
    75721 gttagctacc agccagcttc ccgcgccgtt tatgctatcg acaacattca aatcttccat
    75781 tcaatattcc ttttcaactg actcaaaatg gagaaataca ctccgccgcc ttatgacggg
    75841 cagtctgaca agggtaaaca ataaagcata attttgccaa atagatcaca gatttctcat
    75901 ctgaatcacc ttgcttttgg cgaaaaaatc cccgcagagc ggggctcaga cagatatcaa
    75961 tgtgcgcttt gtcatgccgg atgcggcgtg aacgccttat ccggcctaca aaaacctgca
    76021 aattcaatat attgcaggat acgtgtaggc ctgataagcg tagcgcatca ggcaattttg
    76081 cttttggcga aaaaaagacc cgcagagcgg gccttgagat aagcagaaag gaatatctta
    76141 cagaacgtcg atcgcgttca gttcctggaa tgctttttcc agacgagcaa tcatcgaagt
    76201 ctgaccggca cgcagccata cgcgcggatc gtagtatttc ttgttcggct gatcttcgcc
    76261 tttcgggtta cccagctgac cctgcagata agcttcgttc gctttgtagt agttcagaac
    76321 gccttcccag gttgcccatt gggtatcggt atcgatgttc atttttacta cgccgtagct
    76381 tacggagtct ttgatttcct gagcagtaga accggaacca ccgtggaata cgaagttcag
    76441 gctgttgtgc ggcaggttgt gtttcttgga aacatattcc tgagaatcac gcaggatggt
    76501 cggagtcaga accacgttac ccggcttgta aacaccgtgt acgttaccga aggacgctgc
    76561 gatggtgaaa cgcgggctga ttttgctcag ttcggtgtat gcgtaatcaa cgtcttccgg
    76621 ctgggtgtac agtgcagaag cgtccatgtg gctgttgtcc acgccgtctt cttcaccacc
    76681 ggtgcaaccc agttcgattt ccagagtcat gccgattttg gacatacgct ccaggtattt
    76741 agagcagatt tcgatgttct cttgcagaga ttcttcagac aggtcgatca tgtgagaaga
    76801 gaacagcggc ttgccggtag ctgcgaagtg tttttcaccc gcgtccagca gaccgtcgat
    76861 ccacggcagc agtttcttcg cgcagtggtc agtgtgcagg ataaccggaa caccataatg
    76921 ttcagccatc tggtgaacgt gatgcgcacc agagatcgcg cccaggatag cagcaccctg
    76981 cggaacgtca gatttcacgc ctttaccagc gataaaggaa gcaccaccgt tggagaactg
    77041 aacgataacc ggcgctttaa ctttagcagc agtttccagt acggcgttga tggagtcagt
    77101 accgacgcag tttactgctg gcagtgcgaa gttgttttct tttgctacct ggaaaacttt
    77161 ctgtacgtca tcaccagtga ttacgccagg ttttacgaaa tcaaaaatct tagacatgtc
    77221 tcttgtcctg tatcgtcggg ccttatactc atcatacttc aagttgcatg tgctgcgtct
    77281 gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact ctcttgtcgc
    77341 cttcctgcaa ctcgaattat ttagagtatg aaaaaatgcg tgctctaaaa gcgcgctgaa
    77401 acaagggcag gtttccctgc cctgtgattt tttacttctt agcgcgctct tcgagcatcg
    77461 ctactgcagg cagtacttta ccttccacga attcgaggaa tgcgccgccg ccagtggaga
    77521 tgtaggagat tttgtcagca atgccgaaca ggtcaattgc tgccagagtg tcgccgccgc
    77581 cagcgatgga gaacgcttcg ctgtctgcga tagcgttagc cacgatttca gtacctttgc
    77641 ggaagttcgg gaattcgaac acgccaaccg gaccgttcca cagaatggtt ttcgcattct
    77701 tcaggatttc agccagttcc tgagcggaag catcaccgat atccaggatc tgctcgtcag
    77761 ctttcacatc gttaacagat ttcagggtag ccggtgcagt ttcagagaac tcggttgcta
    77821 cgcgaacatc agacggaacc gggatgttgc aggtggtcag cagacgttta gcttcgtcaa
    77881 ccaggtcagc ttcgtacagg gatttaccca catcgtggcc ttgtgccgcg ataaaggtgt
    77941 tagcgatacc accaccaaca atcagctggt cagcgatttt agacaaggag tccagaacgg
    78001 tcagtttggt agatacttta gaaccaccaa cgatagccac catcgggcga gcaggttctt
    78061 tcagtgcttt acccagcgcg tccagttcag ctgccagcag cgggcctgcg cacgcaacgt
    78121 cagcgaattt accgataccg tgagtagaag cctgcgcgcg gtgagcagta ccgaatgcgt
    78181 ccattacgaa cacgtcacac agtgcagcgt attttttgga cagggtttcg tcgtctttct
    78241 tctcgccttt gttgaagcga acgttttcca gaacaaccag ttcaccttca gcaacgtcaa
    78301 cgccgtcgag gtaatcttta accaggcgaa ccgggttaga cagtttgtct ttcaggtagt
    78361 taacaaccgg cagcagagag aattcttcgt tgtactcgcc ttcggtagga cgacccaggt
    78421 gggaagttac catcactttt gcgccttgtt tcagggccag ttcgatggtc ggcagagaag
    78481 cacggatacg cgcgtcgctg gttactttcc cgtcttttac tggtacgttc agatccgcac
    78541 ggataaatac acgtttccca gcaagatcca gatcggtcat cttaattaca gacatggtga
    78601 atcctctcgt tgattctaaa agttttgcag acgctgcttg cgtcttacct gaaagcaaca
    78661 gtagccatag ctaacgtcgt gtcgagcatt cggttagcaa agccccattc gttatcgcac
    78721 cagaccaacg ttttgatcag gtgtgcgcca ctgacccggg tttgggtgcc atcgacaatg
    78781 gcactgtgcg gatcgtggtt aaaatctaca gagaccaacg gcaattccgt atagtcaact
    78841 ataccatgaa atgcaccttg tgctgctttt tgcagcaaca ggttgacttc attggctttt
    78901 acaggtttct tcaccgtcac gcttaaatcg attgccgtca catttatggt tggcacacgt
    78961 accgcaatcg cttcaaagcg atcgttaaat tgcggaaaaa atcgtgtgat accggcggcc
    79021 agtttagtat cgaccggaat gatcgactgg ctggctgccc gggtgcgacg caggtcagga
    79081 tggtatgcat caataacctg ttgatcgtgc atggcggagt gaattgtggt cacagtgccg
    79141 gactcaatac cgtacgcatc atctaacaat ttgatgacgg gaattatgca attcgtggta
    79201 caggaagcgt tagaaacgat gcggtgttcc gcacgaagtt gatcctgatt gacgccgtaa
    79261 acaacggtcg cgtcgagatc gttactgcca ggatgtgaaa agagcacttt tttggccccg
    79321 gcggcaatat gcgcttcgcc atgctcgcgg gagccatata cgccggtgca gtcgaggact
    79381 acatcaacgc caagttcacg ccaggggagc gattgcagtg aacgttcatg caatacgcgg
    79441 atggcgtcat caccaacaaa aagttgatcg cgttcctgtc gtacttccca tgcaaaacgg
    79501 ccatgactgg tgtcatattt caacaaatgc gccatgcccg cagcatccgc cagttcgttg
    79561 attgccacca cggtaatttc cgcacggcgt cctgattcat acaaagcacg aaccacatta
    79621 cgcccgatgc gaccgaagcc atttatcgct acgcgtacgg tcatagatct cctgcaaggt
    79681 tttccctgag caaatttgcc agacagagta atccagcaaa tcgtccgggg aaaccttacc
    79741 tgtcgcaaac tgcgactgat tggttaattg tcgaacattt aatcgactga aacgcttcag
    79801 ctaggataag cgaaacgtgg aataaaagga atgattgtcc agtcaaagaa aacaaatatc
    79861 tgacttacgt cacgttttca gccccgcccc tgtggaattt attccacttt gcagaatcac
    79921 gaataagcgt agcaaaatct tttcgttgtg ccgtcacaaa ccctgactta tatcagaata
    79981 tgactgatgc agcgtcgata ttatccgcaa tacgtccgag tttcggagta ctccaatgat
    80041 gacattgccc tacccatatt cttcctccgc acgcttttac gcactgcgcc tgctccccgg
    80101 gcaggaagtt ttttcccagc tacacgcttt tgcacagcag cagcaacttc atgccgcctg
    80161 gattgcaggc tgtaccggta gcctgactga cgtcgccttg cgctatgccg ggcaagaagg
    80221 caccacgctc ctgaacggta cattcgaagt gatctcacta aacggcacgc tggaacagag
    80281 tggtgagcat cttcatctct gcgtttccga tccacacggc acgatgcttg gcggtcatat
    80341 gatgcccggc tgtaccgtgc ggaccacgct tgaactagtg atcggtagcc ttgaggaact
    80401 ggcgtttagc cgccaacctt gcgcgctttc tggttacgac gagttgcata tttcccccgt
    80461 taaataacta ctttcagaga ggttttctta tggcgcgtag ccacttttcc agtcaggcac
    80521 tggtgttgat cgttatatct atcgccatca atatgatcgg cgggcaactg gccagcatgg
    80581 tcaaattacc tatatttctc gactccatcg gcacccttat tagcgcggta ctgcttggtc
    80641 cggtgatcgg gatgctaacc ggcttactga ccaatttact ctggggattg ctgaccgacc
    80701 cgatcgccgc cgcatttgcc cccgtcgcga tggtcatcgg cctggtcgcc ggttgcctgg
    80761 cgcgagcggg ttggtttcgc accctgccaa aagtcgttgt cagcggcgtg attattacgc
    80821 ttgcggtaac ggtcgtcgct gtaccgctgc gtactgcgct gttcggcggt gtcaccggta
    80881 gcggagccga tttgttcgtt gcctggatgc actcgatggg gcaaaacctg gtggagtcgg
    80941 tggcgattac ggtgataggc gcgaacctgg tcgataaaat tcttacagcg gtgattgtct
    81001 ggcttttact gcgccagctg ccgattcgca ccacgcgcca ttttccggca atggctgccg
    81061 tgcgctaaat gcatccattt acctcattaa cactgtgggc attggtggcc tgcaccacgc
    81121 ttatcctgcc cgcgcagacc attttgccga tctatagcgc ggcgaccttt ttctgcctta
    81181 ttgcgctaaa ggccacgcgg cggcgggcaa aatatgtcgt ctggctgatg ttctcgctgg
    81241 gagccgggct gtggctggta cacggtggct ggctaccaga atggctaagc ggcacgccac
    81301 gctcgccaga acggtggtcg catgctatta cgctctggtt gcggattctg gcgattgttt
    81361 caacctcaca gttgtggatg cagtacgtcc ccgtgcaatg ttttattcgc gctctgtttg
    81421 cttctcgcct gccgccaggc gtcgcttatc tgtttgccgg gccgttattg gtcgttgagc
    81481 agttaaaacg gcaattagcg attattcatg aagcgcagcg tgcccgcggc gtgccgctgg
    81541 atgagggctg gtatcagcgt ttacgtgcga tgcccgcgtt aattattccg ctaacacaca
    81601 atgcgcttaa cgatctggct gtccgtggtg cggcgctgga tatgcgggca tttcggatcc
    81661 acaaccgacg aacaacgttg tgggcaccag ccgatagcac cctacaacgc gtagcgcgtt
    81721 acaccatgat cttgctgatg ctgacagaat tcggagcgtg gatatggtta cgttagaaca
    81781 gttccgttat tgcccgacac actcaacgca tcctccgttc tgttatgact ttcactatgt
    81841 caaaccgggg atggtcgcca tctttggtga caacggcagt ggcaaaagta cgctggcaca
    81901 gctgatggcg ggctggtatc cggactttct gccaggagag ataacaggca cggggacgct
    81961 cctcggtacg cccattgggc atctcccgct taatgaacaa tccgccacca tccagctcgt
    82021 ccagcaatcg ccctatttgc agctctccgg ctgtaccttt agcgtggaag aagaagttgc
    82081 ctttgggcca gaaaatctgt gtcttgctga agcagagatt atggcgcgga ttgatgccgc
    82141 cctgactctg accgagtgcc agccgttgcg ccatcgtcac ccggcaacac tctccggtgg
    82201 agagacacag cgcgtggtca tcgcctgcgc catcgcgatg cagccaaagc tcttaattct
    82261 tgatgaagcc tttagccgcc tgactccgca ggccagcgaa atgctgttgc agcgcttaca
    82321 acactgggcg tttgaacgtg gttcgctgat tattcttttt gaacgtcatc gcacaccttt
    82381 cctgaactac tgccagcaag cgtggcaatt gcaaaacgga gcgctccagc cactatgtta
    82441 acgttaaata agatatctta tcgctggcct ggcgcggcaa cggattgtct ttgcgacatt
    82501 tctctgcaac tcaaacaagg tgaatggttg gcgctgaccg gcgacaatgg cgcgggaaaa
    82561 tcgacgctgt tacgagtaat ggcaggtctt cttacgccca cagccggtac ggttatgctg
    82621 caacaacagg cgatgaaaaa cctgaaaaac cgccagcgcg cggcaaaggt tggcgtactg
    82681 tttcaggaag cggaaaacca actttttcac agcaccgtcg ctgacgagat cgcatttggt
    82741 ttgaaactgc aaaaatgccc ggcggatgaa atcactcaac gtacccatgc tgccctgcaa
    82801 tgttgtcagt tagcagacac tgccaactcg cacccgttgg atctgcatag tgcgcagcga
    82861 cggatggtcg ccgtcgcctg cctggaagca ctctctccgc cactattgct gctggacgag
    82921 ccgagtcggg attttgacga aaattggttg agcgtgtttg aaagctggct ggagaagtgc
    82981 cgccagcgag gcacgagcgt cgtagcaatc agtcatgacg ccgcatttac ccggcgtcat
    83041 ttctctcgtg tggtgcgact ggaagatggt ctaatcagga acgttaatcc accagacgat
    83101 attcacccgt agcggtcatc attaaggtca aatttgcagg cagactctct tccagcaccc
    83161 ggcgaacatt cggcccgtcg gtacggtcat aaaaagcttc tgcgtctgcc agagataccc
    83221 ctcccgccag cttgcgccct accagtcgtt cccgcaacgc agtagctggc gcgttgataa
    83281 agatcgagaa atcacaaaat tgcgcaagct ggcaccactt ctcgtcgtcc agcaacaacc
    83341 agtttccttc gacaatgacg agtggtgcgg taacgtgcaa cgcatcttca acaggatcat
    83401 gcttttgtcg atcgtactgc ggccacgtac aatccccttc cacgacctgg cgcagatttt
    83461 ccgccagttt cgcaacgtca aatgtctctg gtgcgccttt gaaggggcgc aattgatgcg
    83521 catccagcca gctattgtaa tggtgaaaac catccatcgg gagcgtctga atagcgggca
    83581 gttccgggtc ttgttgcgcg agatattccc agaaggtggt tagggttgat ttacccctcc
    83641 ccggcggagc gcaaagaaaa accaccgtac gccgctgcgg atttaccgtc tgtaaggccg
    83701 ccaacatgtg cagcaacggt ttatgcacgc gttctatctc ttcatcatgg tactgcgctt
    83761 gcacgttcag cccattcacc gttaattcaa ttttcacggc gttaattcct ttaaggtggt
    83821 cgttactgcc agcgtcagcg ccgttttgac cattccgcta aaacgcgcct ctgaccagac
    83881 ggcgaaatcg gcgaacttca tcgctttcaa cgcgtcatag agcgtcgcat tgcaggttat
    83941 gctattgacg ttgctgataa aatcccaggt catgtcatcg gcaaatccgg gcgtttcatc
    84001 ctgttcgttc aggtaatgcc agtactggga aaagtgcgtg atatcggcat acagccattt
    84061 atccattttc cccagcgcat aaatcaaacg cagcgcaaca tcaatatcgt caagcgggcc
    84121 gctttgggcg agtaacggct tcactgcata ttccacgatc tcttcatcgt tatcgacaaa
    84181 cagcgacggc agaaactggc gtatcccctg gagcaaaata gaatgcgcag ttttcataaa
    84241 tgaaaataaa ttgtcctgtg catccagttg ctcaagcaca tcatcttctg tcagcgtcgc
    84301 cattggtctt ccgatagatc accatcaatg cagggagtct attatattac acgtttggtc
    84361 cgcgccgccg atcagtaatg tctgcgtccg caccccattc gccgtctgtt ggataccgtt
    84421 caccaactcc ccgcccgtca cacccgtagc gctaaagagg atatcgttcc ccctcaccag
    84481 ttcatcgagc gagtacacac ggttaacgtc aacgcccatt tctctgcaac gcttgcgctc
    84541 ctgctctgca atctgccgat tttccgtgta atccccttta gcctgacaaa agtcaatcag
    84601 ctcggcctgc atatccccac ctaatgcttt aacggcacag gcagaaatca cgccctccgg
    84661 cgcaccgccg atggtgtaca tcacgtcgta aggattatcc tgccagcagg tcagcacgct
    84721 ggcggcgaca tcgccatccg gcagagcgaa aaccttcacg cccagctgtg ttgcttcttc
    84781 aatcgcggca ctcaggcgcg gtttatccag cgtaaccatg cgcagcttgt ccagcggttt
    84841 acctaacgcc cgcgccacgt tacgcaagtt gtccgccaga ggcaaagaca gatcaatcgc
    84901 cccggcagcc agtcgattaa caaccagctt tttcatatac atatcgggcg catgtaacag
    84961 gctatctcga ggggcgaaag ccatgaccgc cagcgcattg ctctgcccca tcgctaccat
    85021 ccgcgtgcct tcaatcgggt caaccgcgat atcgacttct ggcccatcgc ctttgcccac
    85081 ctcttcgcca atccacagca tcggcgcatg gtcaatttct ccttcgccga taaccactcg
    85141 tccacgaaaa gcgacgtcgt ttaatgcctg gcgcatcgcg gtgaccgcca ggccatcaat
    85201 tttatttttg tcgccacatc cggtttgcgg ccaggcagcc aacgctgcct gttccgtaac
    85261 gcgaaataat ggccacgcca gggacatcat tgcgcggcct ccccgatatc aacgccaaat
    85321 tgtgccagca gatatttctc ggcctgttca ttccagatgc cgtgcgtttc ctcaaccagc
    85381 cgcgccaact ctttaaacag cggatcggtt ttgccttttt cggcaaaatc ggcaatggca
    85441 gtgagcggca tggtcacgcc gttataaatg agttttttac cgccagggat atccggcaga
    85501 ttgagcacgg tatctggcac cgcatccagc ccaccgatat gcgtgaccat aaaggacggc
    85561 tgcaactgcc ccgtggcgct taacgcaatc gcctctttca tgtcgtccgt tgaaccaccg
    85621 gatgtaccga ctacgtgcgt gctgttgtaa tggacgttgt agaaattaaa cggcactttg
    85681 aagtttttat ccgtcggccc ggcaaagaag ttcagacagc catcttccgc cagtaattcg
    85741 tcagccatct caacgacagc aggcaccgcc gcataaacaa aaatgtcatc gaacccgaca
    85801 tctcctgtca gcgcccgcag cgtctggacc ggatcgctca tccctttggt attcacatac
    85861 accagctcaa tgcctttact ggccgccaga tccaccggca gcagcttctg cacctgcgcc
    85921 aggcgtttgt cgtcgatatc gaccaccacc acccgtgacg gttgtatgcc accgttaatg
    85981 gcgtaatcga tagcgccaat gcccatcgga cctgcacacg ccagcagtgc aatattgccg
    86041 ccaggtttga cgcccatgcg atgctcataa acatattgcg tggtgtgata attggcatga
    86101 taagcaccaa taatgcagca cataggctct gccagcgacg ccgcagcaaa ataagagccg
    86161 tgatacggta atacgcagcc caaattaatg gcgatttcgg gaataatcat ataggtggcg
    86221 ttaccgccaa aatattcgta gctgtagcct gctgaatatc cgcttggtaa ccccatcgcc
    86281 ggttgcaata caaaacgctg gcctttttta tatttgccag tgagattttt acccacttcg
    86341 acaataaccc cggcacattc atgcccggta atgaccggat gattttctaa atcgtcgggt
    86401 acgcgtttat gttcactacc gagtaacgcc gctttccagg tcgataaaca gacgctgtca
    86461 gaaattacac tcactaacag ttcattatcg gtaatttctg gcagttcaaa ttcgcgcaga
    86521 cggacatccc gcttaccata aatagcagca actttggttt tcatttttac ctctgtaatc
    86581 agtgtttatg ttcggcggtt aattgattaa ataaagtgtc gagttttgga tcgccaatat
    86641 agttattaat caatatcagc ggtttatccg tcacacgttt cacgcgccct tccagactgg
    86701 cgtgagtgac gacgatatcg gcatccgcag gcacgttttc tatcgcgtaa tgttttactt
    86761 ctattgccag ccccgctttt tccaggcgtt tacggaaggt ggtcgcgccc atcgcactgg
    86821 agcccatccc cgcatcgcaa acaaaggcga tacgcttaac cctgcttaac gagaatgcac
    86881 cctcttgttt catcgcctta accgcattgg ctgactgagc aaactcatct tcgctctccg
    86941 tttccaccgt tttttccatc ttcagtatca gcgaagtaat agcaaaggac accagggtac
    87001 ctaccgtaac accggcaatt gtcgccagga acgagccttt cggcgtcagt gccaggtaag
    87061 caaagataga ccccggactt gggccagcca ccagaccacc gtccagtaag ttaaacatcc
    87121 aggtaccaga cataccgccc gcaatcatgg caataatagt cagcggcttc atcagcacat
    87181 atgggaaata cagttcgtgg atcccaccga ggaagtgaat aatcatcgcc ccgggcgcag
    87241 aacgtttact catcccttta ccaaacaagg taaacgccag cagcagcccc aggcccggac
    87301 ctgggttaga ggccaccata aagaagatgg atttaccgtt aaccgaagcc tgttgcattc
    87361 ccagcggata atagacgccc tgatcgatcg cattattgag gaaaagcact ttcgccggtt
    87421 cattgataac tgacagcaga ggcagataac ccgcatgtac cagcgcctcg atacactctt
    87481 tgacgaaagt attggcaatt aacaccgccg ggccgatcac ttcaaaaccc agcagacaaa
    87541 ggagcatccc cgcgatacct aatgagaagt tattgataac catctcaaaa ccggcaggta
    87601 tgcgtttttc cagcgccttg tcgatatatt taatcaccag accaccgagc ggccccataa
    87661 tcatcgagcc aaggaacatc gggatttcag cgccgacgat cacaccaata gtacctattc
    87721 cgcccatgac cgccccgcgt ttaccgccga ccaggtgacc accggtagaa ccaatcatca
    87781 cgggcaataa ataggtaatc atcgggccga caattttggc gaaatgttca ttcggcagcc
    87841 aaccggtggg aataaataac gcagttataa agccccaggc aataaaagca ccgatattgg
    87901 ggatgaccat tgccgtcaaa aagcccccaa aagcctggac ctttgcacga gcagacttgt
    87961 tttccataat attatcctgt agaggagaga gtaattatcc gcggctgata atgtccgcta
    88021 attgtttttc tgatgatgct gttaagagtt gttcgagtat ttcttcttcg cacagtaatt
    88081 cacttaacgc ctgaatagcg ccaatgtgcg aatcggcatc ggcagcagag agtccgatga
    88141 gtaatttaat tggttcgtca ttccccggaa aataaacatc ttgttcaagt aatgtcaaag
    88201 acatcccggt tttaagcgcc ccacattccg gtcgcgcatg aggcattgcc acgcctggtg
    88261 cgagaatata ataagggcca ttgttaatgg tggaatcttt aattgcctga atgtaattct
    88321 cgctgatata gtttttatcc agcaatgata ccatcgagaa atcgatagct tcctgccaat
    88381 cttttgctga atgtataact gagattgatg attccggaaa ataatcacta agccgcataa
    88441 attatcctta ttttattttt agggtaccgt caggcggtaa aaataaaata ctcgtgcggt
    88501 ttaatgaaag caattgagca gggaaaccga tgttttgaaa acagtgaatt attaatttct
    88561 tatataacat tgagttatag atataacaac aaatgtcaat acgcatatcg tgatgcatat
    88621 tacataattg atatttattg catataaata tttgtgatct acaacacgct ttatctattg
    88681 cttgtccgca aacggacata tcaaggtaat aaaaaaggtc gccgaagcga cctttttacc
    88741 cgaaatgcta attacagcag ttcttttgct ttcgcaacaa cgttatcaac agtgaagccg
    88801 aactcttcaa acagcagctc tgccggagca gattcaccga aggtggtcat accgacgata
    88861 gcaccgttca ggccaacata cttgtaccag tagtcagcaa tacccgcttc tacagcaacg
    88921 cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag cagcatcctg cttgtcaaat
    88981 gcgtcggtag acggcatgga caccacgcgc gctttcacgc cttcggcagt cagtttttcg
    89041 taggcagcaa cagccagttc aacttctgaa ccggtagcga tgaaaatcag ttccggctga
    89101 ccggcgcagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagttg ctcttcagtt
    89161 cgttcctgct gcgccaggtt ctgacgggag aggatcagtg cggtcgggcc gtcctgacgc
    89221 tcaacaccgt atttccacgc gaccgcggat tcaacctggt cacacggacg ccatgtagac
    89281 atgttcgggg ttacgcgcag agaagcgacc tgctctaccg gctggtgagt cgggccatct
    89341 tcgcccagac cgatggagtc gtgggtgtaa accatcacct gacgctgttt catcagcgca
    89401 gccatacgta cggcgttacg tgcgtattcc acgaacatca ggaaggtgga ggtgtacggc
    89461 aggaaaccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa ctcgcgaaca
    89521 ccgtagtgga tgtagttacc cgcagcatct tcgttgattg ctttagaacc agaccacagg
    89581 gtcaggttag acggtgccag gtcagcagaa ccgccgagga attccggcaa cagcggaccg
    89641 aaagcttcga tagcattctg agacgcttta cggctggcga ttttcgccgg gttagcctgc
    89701 agtttagcaa tgaactcttt tgctttcgcg tcgaagtcag acggcatttc gcctttcata
    89761 cggcgggtaa attcagcggc ttcctgcgga taagctttcg cgtaagcagc gaatttctcg
    89821 ttccatgcgg attctttcgc ctggcctgct tctttcgcat cccactgagc atagatttca
    89881 gacgggattt cgaacggtgc atatttccag cccagctgtt cgcgggtcag ggcaatttca
    89941 gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg ggaaccgaaa
    90001 ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg cgcttcttct
    90061 actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac gtgccagccg
    90121 taagcttcga aacgcattgc ggtgtcgtcg gtgaaccagc cttcaacgtg accatcaata
    90181 gagataccgt tgtcatcgta gaacgcaatc agtttaccca gcttcagcgt acccgccaga
    90241 gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa ggcataggtg
    90301 tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt tttttctgca
    90361 atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt ggtttccaca
    90421 ccagcggtat aacctacttc cgggtggccc ggagttttag agtgcagctg acggaagttt
    90481 ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta gatcagcatg
    90541 gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg attctgcggg
    90601 ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc cataggggca
    90661 cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg aatagcattg
    90721 gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg cacgccctta
    90781 acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa tttaccgcaa
    90841 gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg gatcatgctc
    90901 gcatgttgcg caatctactc gcccgtccgc tgcgcttttc cttatactga gactgagcgt
    90961 cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg aagagaaaac
    91021 atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac tggttgccag
    91081 aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc ttacagtttg
    91141 agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga cagcaaggcg
    91201 acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc caatgcgcta
    91261 ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa ggatgtgaac
    91321 gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga tatgatgacg
    91381 gataacgaag tcgaagcggt gatcggtcac gaaatggggc acgtggcgtt aggccatgtg
    91441 aaaaaaggaa tgcaggtggc acttggtaca aatgccgtgc gagtagctgc ggcctctgcg
    91501 ggcgggattg tcggaagttt atctcaatca caacttggtg atctgggcga gaaattagtc
    91561 aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta cgatcttctg
    91621 cgccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact ggcaaaactg
    91681 gaagaaggtc gccaaagctc aatgtttgac gaccatcctg catccgccga acgcgcccag
    91741 catattcgcg atcgtatgag cgcggatggg gttaagtaag tcagacatac cgatctacaa
    91801 cataattgta ggtcggtaac gcctgaatcc ccgatcagag tagtgatttc actttattta
    91861 gcaaaacggc tacatcatcc ggtcgggtat atcccgtatc agggtctata atcgagctgt
    91921 aaacatgtgg cataatgcgc ggcacccccg cctccagaca agttttcaga atcacactga
    91981 agttatccaa atcaatcccg ccagtcggct caatcagcgt cataccattg cgcgccgccg
    92041 tggtcgccag cgcgtatagc tccggaagcg acctctcgcc gcccatcgta aagaacttcg
    92101 ccgcgtgtgc gcccatatct agcatcattc gcaccgccgt atcgcaggag acgcgggcag
    92161 gcgtaccctg gctgctgcta acgccggtgg aaatcagcac ttcgcccggc gtccccgtcg
    92221 ggctaaccag cgcgttgaca tgggtctgct caccgcccgt agccgcaagc gcgcctgccg
    92281 cgaaaccaca accggtaaag gtctggttaa cgtgtgctgg atgaatctgc gccgcaatca
    92341 tcgcagcttt gtaatactgc gccgggtcac ccgcccccag acctactgaa atggacggta
    92401 tttccgccat ccaacgttta acttcctgga tcccctcatc caccgaggag aactgcgcgg
    92461 aaagcacgcc aatcactgcg tggccttcgg cggcgtcaaa gatttcacgg gcattggcga
    92521 tatctttcgc cagcacatta attgccacgc gattacgata gaaattaatc ttttgcatga
    92581 tctgcaatct ccttcagtcg cgccacgata agtgccattt ccccttccgc caccgtacgc
    92641 ggatcaaggc taaaaacgcc ctggtggagg ttataccgcc gtgcataaat cgcgatatag
    92701 ccaccacgca gttgcgcttc gacgacgcga gcgtcaactc ccaattcctg cgcatttact
    92761 cgaatacgaa tacgccagat tgcacgcccg gcttcatcct gctcaatatc agccgttaac
    92821 ccgtgaatcg cagaaatcgc ttcaaccact ggctggagct gttccgcggt gatgactgcc
    92881 tgcccctggt gataattctc cagcgcgtaa accagcccaa ccatgttctc cttgccgatt
    92941 ttcatcgccc tggcaatgcc gtgatgctgc gctttgcagg ctgcaatcca gattttttta
    93001 ccagtgataa acccggaggt cggcgcgtta aaggcttttg cgccgctgta gaccaccata
    93061 tccgctccgc tcaccaccca ggcacgtaaa tcctcttcgg cggcggcatc gacaatcagc
    93121 ggcaggttgt acatctgcgc gacatgtgcg aagtcctcga tgttcaacat tcccttctgc
    93181 acgcagtgat gcgacttcac atacagcagc gcggcggtcg cgtcggtaat ggcactctcc
    93241 agctgccaga gagccgccag gttacttgaa cccacctcca caacgcgccc gccacctaag
    93301 cggatcgcgc tgaggatcga tgcaccgtaa tcgacgttat ggccgcgcag catcaccact
    93361 tcattggcca tgccggagct atccggcatg agcgccaccc gggccttgtc gccgcgtgta
    93421 atggcggcgg cgacagcgat ggcgatccct gctgaggcac aggaggtaac gtagctatcc
    93481 tccgccccgg taaagcgaga gaccagctcg ccggttttgt ccaccagctg gtcaatctcc
    93541 acgaaagagg atgccgcgcg cgcggtggcc tgcatcacct ccggcgcgac gctggagacg
    93601 ccgagaatcg tcatcttgcc gcaggcgtta atcacctgtt tcagtcccag ttgttgatag
    93661 atattctgcg tcatggctta cagcactccc agcagcgagc agaccacgct caacgccaca
    93721 atcgacagca gaatcgtggt gtaacgcgga ccctttttca ccaaatagaa gtagatggcg
    93781 aacactgccg ccagcggcag caggccgggc gcgatggagt ccagaatctg ctgtaccacc
    93841 acttcagagc ctttcagtgc gctaattttt aaaggtgagg ttatcttgac aaagctcgcg
    93901 gatagcgcgc ccatcatgat aagccccagt acgttagcgc cgtagataag ctccttgatg
    93961 cgccctccct gcagcagact gataatcgaa tcgcgcccca gcgtatagcc cttgtgcacc
    94021 atgccgtagc tgattgccag cgtaatcgcc gggtagagaa tcagcggcaa aataccgccc
    94081 atcgcgctgc cattggcggc gaacgggata aaaatagcaa tcaacagcgg catcaccgcc
    94141 gcccagatga tggagtcgcc catcccggcc agcggcccca tcaggccggt tttaatcccg
    94201 gtgatagagg catcgttaat cgcctcgccg cgggtctttt gctcttccat ggcgatggag
    94261 atcccctgga tcaccgcgcc aaacgtctgc tctgagttga agaagttcag atggcgtttt
    94321 aacgcctcca cctgctcctc cttttgcgga tagagctttt tgataatcgg cgtcatcgag
    94381 gcgcagaaaa tcaggctctg taaacgctca taggagctgg aaacttccgc gcccagccag
    94441 tagataaacc acgctttggt gatatccgct ttggtcagcg ccccggactc ccgcgcgcgc
    94501 tcaaccagtt catgctgcat tacgtcgtcg ctcattatgc cgctccttca tttttcgcca
    94561 ggcctttaat caggaaggcc acgcaggtgc cgaaaatcgc catcgccatg atgtccacct
    94621 tgaggtacag caccgcgaag aatccgccga taaaccacgg cagcaggctc tttttaccaa
    94681 tcaccataat ggtgatggcg aagcccagcg ccgggagaat cccgcccatg atttcaaacg
    94741 agtgggtcag ccagtgcggc atcagcttga ggaaactttc cactacgttc tggccgaagt
    94801 agttggcggc gaacaccact gggaaacgta gtaccagccc cagcagcgcc gggtagagaa
    94861 aggcgcagcg cataatggct gacatattgg ccgtttccgc gtgtttatcg gccatatgca
    94921 cccaggccgc atttagcgtg cgacgcaact ggtcgaggaa tacgccgata acgccaaatg
    94981 gaatcgccag tgcaatcgcc aggttcgggt ccatattggc cttaacggcg ataggaatgg
    95041 cgatacaggc cgccagcgcc gggtctgacg gcacattgcc gcctggcgtt gaggtcacac
    95101 ccagatagac caactgcatc cccgcgccga taatcatcgc cgcaggcata ttgccgagca
    95161 gcaggccgac aaagaccgcc accaccactg gctgtaacag catggcggag aaggtatagc
    95221 caaggcgcaa ccgggcaaac cagtaataca gccccatcag gcttgcaaaa agtaaggtat
    95281 ccataatttg ttatcccatg ttcagagggt cagaattttt tcaggatatc gtccagcgac
    95341 tgcggtttat cttccgggat ggtctggaaa acagccttaa tgccgcgact tttcaaatcg
    95401 ttaagaatgc caacgtcttt ttcatccagc gttatgttct ggaacaccgc tttacggttt
    95461 ggcccgccgc ctaaaccgcc aacctgaata tcagccacgt caaagccctg ctgcaccgct
    95521 tcctgaatcg ccgccagcga cgggaacagc accagcacgt tgccgtcgcc cagctggttc
    95581 tctttccacg cggccgcgaa gctctggttg ctaaagcagt ccactttgat attcggcggc
    95641 gcggccatca ggtagatatt tttcataaac ggatcggcgt ccagctcgtc gctgaccacc
    95701 gcaatacggt tggcctgaga ctgccctacc cacttcgtca ccacctgtcc gtggataaga
    95761 cggctgtcaa tgcgacataa cacgatgttt gccatacgat tttccttact gttgtaattg
    95821 acgaatatgg gagacgacgt ccaggcaact gttgcgccca gcttccacca gatcggccac
    95881 gcaggcggtc agtgggccat gttcgcgctt gtccagcgcc tccagcagca gcgaggcatt
    95941 aagcccggag atcaccgcca ccggataatc ggcgctcagc cgcgccgcca cgttagaggt
    96001 ggtgccgccg ataaaatcgg tcacgataag cgaaccttcc ggcatggttt tcaccacggc
    96061 ttcgacgcgc tggtaaaact cgcccagcgt atcgacgggc atcagcgcga tttccgtcac
    96121 gccttcgatt tcgcccatca ccatgcgcag actgttgcag agctgcagcc cccagccgcc
    96181 gtgagttagc agcagaattt caggtaacgg atgaataggt acagttgtgg tcaaaatagc
    96241 ctcctttgct ccagtgttta catttcatca ggagcaaagg acgtgccaga atttgtgtca
    96301 cgcacagagg aaatgagatt ggatggggcg ttaagccccg taaaccaggg gatcagctgt
    96361 agagcagttc gtagatataa aaatattctg cgtccgataa acggatagcg taagcctctt
    96421 caatcggcaa aaaggaggat ttgatgacgc taaaggcacg agcgtccaga tcaagccggt
    96481 tttctagcgc catctgtaac ggtttgcggt taatcacgat acgctcgacc atgcagcagc
    96541 agtggatcag aaaacgtagt gtcacctggc ggctcggttt cagggagagc gtgttggtta
    96601 agtagttcag aacatcctcg atctctttca gaattcgctg cgggttgagc accgaaatat
    96661 ggttgatgat gctctccatg gtcagcgcgc tgataaagcg catggcgctg cgctccatct
    96721 ccagtcggcg ctcgctgctt gagagatccg gcgtcagcag gctgagcacc agctccggcc
    96781 cttgctcgga aaacagttcc tccagcgaga taaacggtat atccggcagt cccggctgga
    96841 aggtgccgac gatccctgcc agccgttcac tggcattaag cgcctgctga atacgctcca
    96901 ggctgcgcac ctcgttatag tcgagaatca ccatacgcgt gtcctgcgac atcagctcgc
    96961 caaagctctc ctccagcacc tttttgatct tttccgccgt ccccattccg gttatacatg
    97021 agataaccag cactttgcca ccgctctctt gctgcggcgt gcagagccga caagggatat
    97081 ttttactctg cataagcaca gccaattgcg atagatcgcc agtttcgtag cttaaatcca
    97141 gcccaacctc cagcaggctg gtcagagtga tatttggcaa caacagaacg tcaatctgga
    97201 acagcttgct gatggtgctg ccaaaatgga ccagcgatcc gatatccacc atcagtatca
    97261 gccgctgcca gcaccggtcc tgaatcatcc gggtcagggt ttccagggtg tcatgcaccg
    97321 actgctcaaa tggcatatca attgcgtaga acagatcgcg ctccagcacc cggttcacat
    97381 attgcgcctg gctcgttgcg gtggtcgcac cgtgagcgat tagaatcacg ccgcaatccg
    97441 ggctggtgtc gatgcgctgg cggtagtggc gacactcttt caggaacagg cacagccaga
    97501 ccacctccgt cgccgggcac tgaatatgca acaactcatt gattttgcgg cacagaagcg
    97561 tggcattgtc atactcatct ttacagcgat ccagaatcag gctggatgaa tagagttgag
    97621 gtataagccc tcgccgcaca tagccaatca gcgcaagaaa gtgtttacgt aacgggttcg
    97681 ccaggttttc tggcaatgta aatcctaata cctgctccac gtaaccgatc agcagcgtca
    97741 cacgctcctc aatttgatcg ccatagcgcg gtggatgcgc cgcactatgg cggctataaa
    97801 gaccatattc aaaaatcgaa ctgagtttgt ttttcaggat agccagcgtt tctgcgggcg
    97861 gcacgttact gttgcgcaga ttgacatatt cacgagtcag gaaactgtaa aacaggtcgc
    97921 tctcttctat ttccgcgctg gtcgccaacg aattttttaa cgcgggcaac gtacgcgcat
    97981 ccacgttcag acgatcttta ccgccaaaca acgcatcgac cagcagacgc tgctctggcg
    98041 tggtattaaa cgacatctcc gccagtcgtt tatctaactc tagcgtatca ttacgctcgg
    98101 tcattcccga cgcccacgcc tgcgcgcaca agaactgaat atcactttta agctggccga
    98161 tattgccttc caggggttta cttaacaacc aatgcaagag cgttttatca aggcttatgg
    98221 tacgttcaat tttacgactt tcacgctgaa agaagcttac aatcaattcg agctgctcct
    98281 cgaccgaacg ctggcgaata cccggtaagt caatacatac ctggatacgc cgctggaagg
    98341 tacgcaatag tgccgagttg accgactctg tagtggcgca aataaggcgc accgaaattg
    98401 aatatgttgg cccacttgaa ccaagtgggc ggtattcacc tttatcgaga atagaaaaga
    98461 gcttctcttg tccttcgtac gggaggcgat gcacttcatc cagaagtaaa taacctccgt
    98521 cagcttgctc caccaatccc gttttattct cacttgcccc ggtaaatgcc cccttatggt
    98581 gaccaaacag atgtgaggaa agcaactccg gattatgagc atattcggca cagttaaagt
    98641 aaaccaacgg aggtggcgaa cctatggtgt gcttgcaagc gaagcaatgc attaactcag
    98701 caaagaatgt tttgccaaca ccagatgcac cggttaacag cacatgtagt ccgtggggat
    98761 aaagcaccgc tgcgcggcct ttttctaccg catcacggag actacggtcg tatccgataa
    98821 gtccggaaaa cggatcatca tctgtatatt tttctttctt tggcaataaa ctagccactg
    98881 aatgaactat gcattcgcaa tcatccagtt ttcgattaat gagcaattct agtgcgtgac
    98941 gatgtagaaa aaaaacagga cgtccctgac tttttaccac caggccttca ttccataatt
    99001 gattaagatc tttactaacc gagttgcgtg ccagcccaag gttaaaacca atcgcctcag
    99061 cagtaaatgc tttctccttc gctaaatcag acagttccag cccatacgtc agccgctcca
    99121 gctccttgtg aataacatca atccgtctca tttctttacc gcttaaaaaa caatagttgt
    99181 tcagaacata cactatttgt aaacgtaaga atgagaacta tcgggaagaa ttaatgttcc
    99241 ttgtggcaat ctggtttctg aatattcgtg ttttaaatat tacttatggt ctgaaacaac
    99301 caggcaggtg aacagagcta agaaaaagta gcgtagaaat ataaaaacat atatcgcact
    99361 gatgaatgtt tcccggcatg actatgcaca ccgggaaata tttagtaaat tactcgccct
    99421 ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcggtga
    99481 tttccgactg atcatatgcc ggagccactt ccactacgtc catcccaacg atgttgagat
    99541 ctttcaggcc gcgtaccagt ttaatagcgc gatcggaggt caggccgcca atcactggcg
    99601 taccggtgcc tggtgcaaaa gcaggatcca ggcagtcgat atcaaaggtc aggtaaaccg
    99661 gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgcta cgatcgttca
    99721 cctggcaggc gtccagtacg gtaaagccgt tgtctttatc aaactcggta cgaataccaa
    99781 tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag aacatggtgc
    99841 cgtggtcaaa ttcacaaccg ttcgcgtagg tatcggtgtg ggcgtcaaag tgtaccagcg
    99901 ccattttacc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac
    99961 caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct
   100021 tctcgctcat ctcacgggca tcgccgaaag catataccag atcaccgcag tccacgacgt
   100081 tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg
   100141 tcgaaacctg acggatagct gccggaccgt ggcgaccacc cgcacgacca gaagtggcca
   100201 tatcgaacgg cacgccagta atcacccagt cagcatcgct gtcatatggc tggaagttca
   100261 tcggcaggcg taaaaaacca aaggcgttgg aaaccagtga gttatcgtat tgatgaccta
   100321 aggtgctcat ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt
   100381 aggcccgacg aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa
   100441 atgggcgagg catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt
   100501 tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc gttttgcgcc gggccttgta
   100561 attcaaaggc ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg
   100621 ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc ttcagtatgt ttatctttgc
   100681 ggaatccttt aatcgctaac gaatctccgg cattcacaat atagccgttg ttattcagcg
   100741 aaccttgctt gccgttcagc acatccaggc catcaaccgt agcaacgatc tcgtagttgg
   100801 tgttacgact gtaattacga acatataatt gataattcat tccgactctg gcgtggacaa
   100861 tatagcgtgg ttcattattg gatgaatcat aaacacgcgt aataggtaga gaattaaaat
   100921 taccatcgcg aatagcatat tccagttcgt taatacgaat gctataaacg cggtcgtagc
   100981 cggaaggaac attagtcgaa taactaataa cgatcatctc cgccggatcg cgccaggctc
   101041 gctcagcaga tacgccctga accgttgagt gcacatcccc accccaggcg gtgccgacat
   101101 tacccgatgc actgctatta tactgcgcat tactggaaca accggccagc agcacgatta
   101161 atgccaccaa cgcgctacgt actttccatt ttttcataac cgttccctct gcatttaatt
   101221 aatgcagcga ttgtagggag attatttaat aagaaaagca gctaaattat ttttaagaat
   101281 atataaaaag aaagcggacc gatttacgta aataaatcga cccgccagca gaataatctt
   101341 aattattcat cttcaagata ggtataaccg tacaaacctg cctcgaactc ttcaaggaac
   101401 tgttgttgca gttcagcatc aagatcggtt ttcttcactt gatcacggaa ctgagttaac
   101461 agcgttttcg gatcgagctg tacatattgc agcatgtccg ccacggtatc gccttcgtca
   101521 gacagttcca cttcgacact accgtcaggg aacacgaaca cgtcaaccgc ttcggtatca
   101581 ccgaacaggt tgtgcatgtt gccgaggatc tcctgatatg cgccgaccat aaagaaaccg
   101641 agcatcggcg gattctctgg atcgtactcc ggcataggca ttgtcgtggc aataccgtca
   101701 ccatcaatat agtggtcgat agcaccgtca gagtcacagg taatatccag cagcacagcg
   101761 cggcgttccg gcacttgatc cagcccttcc agcggcagaa ccgggaacaa ctggtcgatc
   101821 ccccacgcat ccggcatcga ctggaacagc gagaagttga cgtacatttt gtccgccata
   101881 cgttcctgca gctcgtcgat aatcggacga tgagcacggt tttgcggatc cagctgcttt
   101941 tgcacttcat ggcacatgct caaataaagc tgctcagccc atgcgcgttc ttgcaggcta
   102001 aaggtgccgg aagagtagcc gatatgaatg tcgtgcagat ccatctgact gtcgtgtaac
   102061 cattcacgca gagaacggcg agttcccggt tcgtgcatct cctgccaggt ttcccacatg
   102121 ctttgcagcg cacgcggcgc atcttctgca ggcgcggtcg gcaccgtgta ttcgttacgt
   102181 tccacgccga tgatattaga caccagcacg gtgtgatgcg cagtcactgc acgacccgat
   102241 tcagtgatta ccgtcggatg cggcagacca ttttcttcac acgcatcgcc aatcgcccag
   102301 atgatgttgt tggcgtattc attgaggcca tagtttacgg agcagtcaga ctgcgaacgg
   102361 gtgccttcat agtccacgcc cagaccgccg ccgacgtcga agcactgaat attgacgccc
   102421 agcttgtgca gttccacata gaaacgcgcc gattcacgaa cgcctgtggc gatatcgcga
   102481 atattcgcca tctgcgaacc gaggtggaag tgcagtagtt gcaggctgtc gagacgcccg
   102541 gcttcacgca gggtttcaac cagttgcagt acctgagtcg cagccaggcc gaacttcgat
   102601 ttttccccgc cggaggactg ccatttaccc gagccctgcg aagccagacg tgcacgcacg
   102661 cccagacgag gaacgacatt cagacgttct gcttcatcca gcacaatggc gatttctgac
   102721 atcttctcaa tgaccagata gaccttgtgc cccatcttct cgccaattaa tgccaggcgg
   102781 atatattcgc ggtctttata accgttgcag acgatgacgc tacgggtcat gccagcatgc
   102841 gccagcaccg ccatcaactc tgctttcgag cctgcttcca gccccaacgg ttcgccagaa
   102901 tgaatcaggg actcaatcac gcggcggtgc tggttaactt tgatcggata aacaaggaag
   102961 taatcgccgt tatagccgta ggattcccgc gcacgtttga acgcggcgtt aatggaacgc
   103021 aaacggtgct gcaggatctg tgggaaacag aacagtgcag gcagacgctg accctgtgct
   103081 tcacgagttt tcactaactg cgcgagatcg acgcgagctt ccgggacgtc cgggtccggg
   103141 cacacgctga tgtggcccag ctcgttaacg tcatagtagt tattgcccca ccaggcaata
   103201 ttgtaagtac gcagcatctt gctggcttcc tgggagctca ttgcaacctc ctgcatggag
   103261 cgtagtacac cgtgttcgcc cgctgacgaa ggcaaaccca tagacatgtc gtcagacata
   103321 gcgaacctca aattatttta ttaagtgtaa aacagttaac gactatcgca gcctggaact
   103381 gcgataacaa cccataagca catggatttt ccagcagtga atgctgacgc tccaactgcg
   103441 caaccggttt ctttttcata acattattaa gcacataacc gaacgtaagt gtgacagttc
   103501 ggcgaaacca cgagaaaact cttgttttta caagagcgcc cttgttcagt cctcagtaac
   103561 tgtaaccagc tcttgaatcc tgagaagcgc cgagatgggt ataacatcgg caggtatgca
   103621 aagcagagat gcagagtgcg gggaacgaat cttcaccaga acggtgagac aggttaagca
   103681 gcagacaacg gttcattatt tcgtatcacc tccacggccg cctgttaaga cgaacccaca
   103741 agccaaaact ctgatttcaa cccggctgga agtggcaaca cgaaagaaac gtcgtgtgct
   103801 ttttatttaa gccgcgcgcc gcgttttata ccccacaatg gcagaaaatt gcaaaagata
   103861 aatacgcaga atgccggcat tgtcaggaaa aatttccagc cacgttttta acagaatgag
   103921 acacgattca aaaaaaagtg gaaatcgggt gaagaattga cctaaaatag ccatccagat
   103981 gttaatccat ccataccgat taacactcag actgccagtg tttttaactt gcagagtcgt
   104041 ggtaggatcc gctaccacag aaaatccaca caacagtttg agctaaccaa attctcttta
   104101 ggtgatatta aatatggcaa aacacctttt tacgtccgag tccgtctctg aagggcatcc
   104161 tgacaaaatt gctgaccaaa tctctgatgc cgttttagac gcgatcctcg aacaggatcc
   104221 gaaagcacgc gttgcttgcg aaacctacgt aaaaaccggc atggttttag ttggcggcga
   104281 aatcaccacc agcgcctggg tagacatcga agagatcacc cgtaacaccg ttcgcgaaat
   104341 tggctatgtg cattccgaca tgggctttga cgctaactcc tgtgcggttc tgagcgctat
   104401 cggcaaacag tctcctgaca tcaaccaggg cgttgaccgt gccgatccgc tggaacaggg
   104461 cgcgggtgac cagggtctga tgtttggcta cgcaactaat gaaaccgacg tgctgatgcc
   104521 agcacctatc acctatgcac accgtctggt acagcgtcag gctgaagtgc gtaaaaacgg
   104581 cactctgccg tggctgcgcc cggacgcgaa aagccaggtg actttccagt atgacgacgg
   104641 caaaatcgtt ggtatcgatg ctgtcgtgct ttccactcag cactctgaag agatcgacca
   104701 gaaatcgctg caagaagcgg taatggaaga gatcatcaag ccgattctgc ccgctgaatg
   104761 gctgacttct gccaccaaat tcttcatcaa cccgaccggt cgttttgtta tcggtggccc
   104821 gatgggtgac tgcggtctga ctggtcgtaa aattatcgtt gatacctacg gcggcatggc
   104881 acgtcacggt ggcggagcat tctctggtaa agatccatca aaagtggacc gttccgctgc
   104941 gtacgcagca cgttatgttg caaaaaacat cgttgctgct ggcctggccg atcgttgtga
   105001 aattcaggtt tcctacgcaa tcggcgtggc tgaaccgacc tccatcatgg tagaaacttt
   105061 cggtactgag aaagtgcctt ctgaacaact gactctgctg gtacgtgagt tcttcgacct
   105121 gcgcccatac ggtctgattc agatgctgga tctgctgcac ccgatctaca aagaaaccgc
   105181 agcatacggt cactttggtc gtgaacattt cccgtgggaa aaaaccgaca aagcgcagct
   105241 gctgcgcgat gctgccggtc tgaagtaatc tttcttcacc tgctttcaaa ggccagcctc
   105301 gcgctggcct ttttctttta cctcaccgcg gtggataggc attcacgccg catccggcaa
   105361 aaagctgccc gcacaataac atcattcttc ctcaacacgt tttatcgcaa attatcatca
   105421 ctactgaaac cgattacacc aataacacca accaaatatt tgtaatattt tcatattatt
   105481 attcagtttt cacagttgtt acatttcttt tcagtaaagt cttaattgca gataacagcg
   105541 tttaatctat gatgatataa ctcaattatt ttcatgcact taaaccataa ctaagataaa
   105601 tgttagtgta agcgattaca ctgatgtgat ttgcttcaca tctttttacg tcgtactcac
   105661 ctatcttaat tcacaataaa aaataaccat attggagggc atcatgcctg acgctaaaaa
   105721 acaggggcgg tcaaacaagg caatgacgtt tttcgtctgc ttccttgccg ctctggcggg
   105781 attactcttt ggcctggata tcggtgtaat tgctggcgca ctgccgttta ttgcagatga
   105841 attccagatt acttcgcaca cgcaagaatg ggtcgtaagc tccatgatgt tcggtgcggc
   105901 agtcggtgcg gtgggcagcg gctggctctc ctttaaactc gggcgcaaaa agagcctgat
   105961 gatcggcgca attttgtttg ttgccggttc gctgttctct gcggctgcgc caaacgttga
   106021 agtactgatt ctttcccgcg ttctactggg gctggcggtg ggtgtggcct cttataccgc
   106081 accgctgtac ctctctgaaa ttgcgccgga aaaaattcgt ggcagtatga tctcgatgta
   106141 tcagttgatg atcactatcg ggatcctcgg tgcttatctt tctgataccg ccttcagcta
   106201 caccggtgca tggcgctgga tgctgggtgt gattatcatc ccggcaattt tgctgctgat
   106261 tggtgtcttc ttcctgccag acagcccacg ttggtttgcc gccaaacgcc gttttgttga
   106321 tgccgaacgc gtgctgctac gcctgcgtga caccagcgcg gaagcgaaac gcgaactgga
   106381 tgaaatccgt gaaagtttgc aggttaaaca gagtggctgg gcgctgttta aagagaacag
   106441 caacttccgc cgcgcggtgt tccttggcgt actgttgcag gtaatgcagc aattcaccgg
   106501 gatgaacgtc atcatgtatt acgcgccgaa aatcttcgaa ctggcgggtt ataccaacac
   106561 taccgagcaa atgtggggga ccgtgattgt cggcctgacc aacgtacttg ccacctttat
   106621 cgcaatcggc cttgttgacc gctggggacg taaaccaacg ctaacgctgg gcttcctggt
   106681 gatggctgct ggcatgggcg tactcggtac aatgatgcat atcggtattc actctccgtc
   106741 agcgcagtat ttcgccatcg ccatgctgct gatgtttatt gtcggttttg ccatgagtgc
   106801 cggtccgctg atttgggtac tgtgctccga aattcagccg ctgaaaggcc gcgattttgg
   106861 catcacctgc tccaccgcca ccaactggat tgccaacatg atcgttggcg caacgttcct
   106921 gaccatgctc aacacgctgg gtaacgccaa caccttctgg gtgtatgcgg ctctgaacgt
   106981 actgtttatc ctgctgacat tgtggctagt accggaaacc aaacacgttt cgctggaaca
   107041 tattgaacgt aatctgatga aaggtcgtaa actgcgcgaa atcggcgctc acgattaatc
   107101 tcctcaggct tcctcccgtc gcggaggaag ccacctcttg cagtcatctt ttcttcgctc
   107161 tatcctctgc cgctatgaaa acatcccgtc tccctatcgc catccaacag gccgttatgc
   107221 gccgcctgcg ggaaaaactc gcccaggcca acctgaagct agggcgtaac tacccggagc
   107281 caaaactctc ttacacccag cgcggaacct ccgccggaac ggcctggctg gaaagctatg
   107341 aaattcgcct caatcccgtt ttgctgttgg aaaacagtga agcttttatt gaagaagtgg
   107401 taccgcacga actggcacat ttgctggtat ggaaacattt cggccgcgta gcgccacatg
   107461 gcaaagagtg gaagtggatg atggaaagcg tgctgggtgt tcccgcccgt cgtacgcatc
   107521 agttcgaact gcaatccgtg cgtcgcaaca ccttccccta ccgctgcaag tgccaggagc
   107581 atcagcttac cgtacgccgc cataatcgcg tagttcgtgg cgaggccgtc tatcgctgtg
   107641 ttcactgcgg tgaacagctg gttgcgaaat aaccatctga actatcagga actttcctga
   107701 tctggctgat tgcataccaa aacaactttc gctacgttgc tggctcgttt taacacggag
   107761 taagtgatgt accgttattt gtctattgct gcggtggtac tgagcgcagc attttccggc
   107821 ccggcgttgg ccgaaggtat caatagtttt tctcaggcga aagccgcggc ggtaaaagtc
   107881 cacgctgacg cgcccggtac gttttattgc ggatgtaaaa ttaactggca gggcaaaaaa
   107941 ggcgttgttg atctgcaatc gtgcggctat caggtgcgca aaaatgaaaa ccgagccagc
   108001 cgcgtagagt gggaacacgt cgttcccgcc tggcagttcg gtcaccagcg ccagtgctgg
   108061 caggacggtg gacgtaaaaa ctgcgctaaa gatccggtct atcgcaagat ggaaagcgat
   108121 atgcataacc tgcagccgtc agtcggtgag gtgaatggcg atcgcggcaa ctttatgtac
   108181 agccagtgga atggcggcga aggccagtac ggtcaatgcg ccatgaaggt cgatttcaaa
   108241 gaaaaagctg ccgaaccacc agcgcgtgca cgcggtgcca ttgcacgcac ctacttctat
   108301 atgcgcgacc aatacaacct gacactctct cgccagcaaa cgcagctgtt caacgcatgg
   108361 aacaagatgt atccggttac cgactgggag tgcgagcgcg atgaacgcat cgcgaaggtg
   108421 caaggcaatc ataatccgta tgtgcaacgc gcttgccagg cgcgaaagag ctaacctaca
   108481 ctagcgggac tctttttgtt aacccctacc ccacgcgtac aaccgcgtgg ggagacgacg
   108541 cggattttta actatgcgta tcccccgcat ttatcatcct gaaccactga ccagccattc
   108601 tcacatcgcg ctttgcgaag atgccgccaa ccatatcggg cgcgtactgc gcatggggcc
   108661 ggggcaggcg ttgcaattgt ttgacggtag caaccaggtc tttgacgccg aaattaccag
   108721 cgccagcaaa aaaagcgtgg aagtgaaggt gctggaaggc aagatcgacg atcgcgaatc
   108781 tccgctgcat attcacctcg gtcaggtgat gtcgcgtggt gaaaaaatgg aatttacgat
   108841 ccagaaatcg atcgaactcg gtgtaagcct cattacgcca cttttttctg agcgctgcgg
   108901 cgttaaactg gatagtgaac gtctgaacaa gaagctccag cagtggcaga agattgcaat
   108961 tgctgcctgt gagcagtgtg gtcgtaaccg ggtgccggaa atccgcccag cgatggatct
   109021 ggaagcctgg tgtgcagagc aggatgaagg actgaaactg aatcttcacc cgcgcgccag
   109081 taacagcatc aatacgttgc cgttaccggt tgaacgcgtc cgcctgctga ttggcccgga
   109141 aggcggttta tcggcagatg aaattgccat gactgcccgc tatcaattta ctgatatcct
   109201 gttgggacct cgcgttttgc gtacagagac aactgcgctc accgccatta ccgcgctaca
   109261 agtacgattt ggcgatttgg gctaacggag aagaataatg atcaagctcg gcatcgtgat
   109321 ggaccccatc gcaaacatca acatcaagaa agattccagt ttcgctatgt tgctggaagc
   109381 acagcgtcgt ggttacgaac ttcactatat ggagatgggc gatctgtatc tgatcaatgg
   109441 cgaagcccgc gcccataccc gcacgctgaa cgtgaagcag aactacgaag agtggttttc
   109501 attcgtcggt gaacaggatc tgccgctggc cgatctcgat gtgatcctga tgcgtaaaga
   109561 cccgccgttt gataccgagt ttatctacgc gacctatatt ctggaacgtg ccgaagagaa
   109621 agggacgctg atcgttaaca agccgcagag cctgcgcgac tgtaacgaga aactgtttac
   109681 cgcctggttc tctgacttaa cgccagaaac gctggttacg cgcaataaag cgcagctaaa
   109741 agcgttctgg gagaaacaca gcgacatcat tcttaagccg ctggacggta tgggcggcgc
   109801 gtcgattttc cgcgtgaaag aaggcgatcc aaacctcggc gtgattgccg aaaccctgac
   109861 tgagcatggc actcgctact gcatggcgca aaattacctg ccagccatta aagatggcga
   109921 caaacgcgtg ctggtggtgg atggcgagcc ggtaccgtac tgcctggcgc gtattccgca
   109981 ggggggcgaa acccgtggca atctggctgc cggtggtcgc ggtgaacctc gtccgctgac
   110041 ggaaagtgac tggaaaatcg cccgtcagat cgggccgacg ctgaaagaaa aagggctgat
   110101 ttttgttggt ctggatatca tcggcgaccg tctgactgaa attaacgtca ccagcccaac
   110161 ctgtattcgt gagattgaag cagagtttcc ggtgtcgatc accggaatgt taatggatgc
   110221 catcgaagca cgtttacagc agcagtaacc caccttagcg agaaggatct cgttgagact
   110281 ctgagtgaca gcgcccttct ttccacgcat actgggcgct gttgcttttt tgaaccagga
   110341 aacagaacct ctgacaatga atttacagca tcactttctt attgccatgc ctgctctcca
   110401 ggatccgatt ttccgtcgtt ccgtggtcta catttgcgaa cataatacca atggtgcaat
   110461 ggggatcatc gtcaacaagc cgctggaaaa tctcaaaatt gaagggattc tggaaaagct
   110521 gaagatcacg ccggagccgc gtgatgaatc aatccgtctg gataaaccgg ttatgctcgg
   110581 cggtccgctg gctgaagatc gcgggtttat tttgcatact ccgccctcca attttgcttc
   110641 cagcattcgc atttcagaca acacagtgat gaccacttcc cgcgatgtgc tggaaacgct
   110701 cggcaccgat aaacaaccgt ctgacgtatt ggtggctctg ggttatgcct cctgggaaaa
   110761 aggtcaactg gaacaagaaa ttctcgataa cgcgtggcta acggccccgg cggatctgaa
   110821 tattctgttc aaaacgccga ttgccgaccg ctggcgcgag gcggcaaaac tgattggtgt
   110881 ggatattctc accatgcctg gtgtggcagg gcacgcctga tgagtggaac cttactcgcc
   110941 ttcgacttcg gcaccaaaag cattggcgta gcggtcggcc aacgcattac cggcactgct
   111001 cgccctttgc ctgcaattaa agcacaggac ggtacgccgg actggaacct catcgaacgt
   111061 ttactgaaag agtggcagcc ggacgaaatc atcgtcggtt tgccgctgaa tatggacggc
   111121 accgagcaac cattgactgc cagagcgcgt aaatttgcca accgtattca tggtcgtttc
   111181 ggtgttgaag taaagctcca tgacgaacgt cttagcaccg tggaagcccg ttccggtctg
   111241 tttgaacagg gcggctatcg ggcgctcaac aaaggcaaag tcgactctgc ctctgcggtt
   111301 attattctcg aaagctattt cgagcaggga tattaaggcg atctaaaagc ctggcggata
   111361 gtcgttaccg ccatcttgcc ggatgcggcg tgaacgcctt atccagtaca catctggcag
   111421 cggctgtagg tctgataaga cgcgttagcg tcgcatcaga catttattgc ctttataggc
   111481 cctataaact tgcgcatcgg gcatggcaac gtcacaaacg cccttccccc acccgctgct
   111541 gataactctg ctgaaacgtt aacatcccca cctgctgccc ggtttgaata acatgcggta
   111601 actggtgggt tttcccttcg cgaatcaaat tccccaccgc gggagtgtta atcaataatt
   111661 caaacagcgc cacgcgtcct tcctgtttat ccacttccag cttttgtgac aacacggccc
   111721 gtaaactacc tgccagttga ttacgtacgg gatctttttc ctgcgccgga aatgaatcca
   111781 ccagtcgctc aactgcctgc gcggcaccac gcgtatgtaa tgttgccagc accaaatgcc
   111841 cggtttctgc cgccgtcagt gccagacgga ttgtctcgct gtcacgcagc tctccgagca
   111901 aaatcacatc aggatcttcc cgcaatgcgg cccgcaatcc cgatgcgaac gtcatacagt
   111961 gcaaaccaat ttcccgctgc tggatcaaac atcgctggct ggcatagaga tattccacag
   112021 gatcttccag cgtcagaata tgcgcatcgg catgttgatt aagatagcca accatcgccg
   112081 ccagcgtggt agatttgcca ctccccgtcg cccccgtcac cagaatcagg ccattctcgc
   112141 tcttgagtaa ttccggcaat accggtggcg caccaagctg ttcgagctgt ggacagtgcg
   112201 aaggtaacaa ccgtaatgcc agagaaatac cttgccgttg cgcgaacgca ctgccacgca
   112261 accgctggtt ttccgccagc gacacggcaa aatccagctg gccattttcc agcaatattg
   112321 cccgctgatc gtcatccagc cactcccgca gtagctcttc gacgtccggc gcatcaaacg
   112381 gcgcagcttc cattagcccg cgaatgcgcc atcgtgcggg ccaggcgctg cacaggtgta
   112441 gatccgagac attatgcttt acactaaggg ccacaatttc ttccatattc atactaagat
   112501 cctcggaaaa tgaacgatat tgcgcataac ctggcacagg tccgggacaa aatctcagct
   112561 gctgcaacgc gttgcggccg ttctccagaa gaaattacgc tgcttgcagt cagtaaaaca
   112621 aaacctgcga gcgccatcgc agaagccatt gatgccgggc agcgtcaatt tggtgaaaac
   112681 tacgttcagg aaggggtaga taaaattcgc cactttcagg aactgggcgt aacaggatta
   112741 gaatggcatt ttattggccc gttgcagtct aataaaagcc gcctggtggc agagcatttc
   112801 gactggtgtc ataccatcga ccgtttgcgc attgctaccc gcctcaacga tcagcgcccg
   112861 gcagaacttc cccctcttaa cgtactgatt caaattaata ttagtgatga aaacagtaag
   112921 tccgggattc aactggcaga actggacgag ctggcagcgg cggtcgctga actaccgcgt
   112981 ttacgtctgc gcgggctgat ggcaatccct gcgcctgagt cagaatatgt aaggcagttt
   113041 gaagttgcac gccaaatggc tgtagcattt gccggactga aaacgcgcta cccgcatatc
   113101 gacacgctct ctctgggaat gtcggacgat atggaagccg ccattgcggc aggtagcacg
   113161 atggttcgta tcggcactgc aatttttggt gcgcgtgatt actctaaaaa ataaggaatt
   113221 aaaggaacgc catgaatacg ttgactttcc tgctttcaac ggtcattgag ctgtatacca
   113281 tggtgctgtt attacgcatc tggatgcagt gggctcattg tgatttttac aaccccttct
   113341 cacagtttgt agtgaaggta acgcagccaa ttatcgggcc actgcgccgc gttattccgg
   113401 caatgggtcc cattgatagc gcctcgctgc tggttgcgta cattctcagt tttatcaaag
   113461 ccatcgtgct gtttaaagtg gtgaccttcc tgccaatcat ctggattgcc ggtttactga
   113521 ttctgctgaa aaccatcggc ctgctgattt tctgggtcct gctggtgatg gcgattatga
   113581 gctgggtaag ccagggtcgt agtccgattg aatacgtgct gattcagctg gccgatccgc
   113641 tgctgcgccc gattcgccgc ctgctaccgg caatgggtgg gattgatttc tcgccgatga
   113701 tcctcgttct gctgctgtat gtcatcaata tgggtgtcgc agaagtatta caggcaaccg
   113761 gaaacatgct gctgccgggg ctgtggatgg cgttatgagt gccgtaacag ttaatgatga
   113821 cggtctggtt ttacggcttt atattcagcc gaaagccagc cgtgattcta ttgtcggttt
   113881 acatggcgac gaagttaaag tcgccattac cgcgccgccg gttgacggtc aggccaacag
   113941 ccatctggtg aagtttctcg gtaagcaatt ccgggttgcc aaaagccagg tggtgattga
   114001 aaaaggcgaa cttggccgcc acaaacaaat taaaatcatt aatccgcaac aaatcccgcc
   114061 agaaatcgcg gcgttaatta attaggtatc ctatgcaaaa agttgtcctc gcaaccggca
   114121 atgccggtaa agtgcgtgag ctggcgtcgc tgcttagcga cttcggtctt gatatcgtgg
   114181 cccaaacaga cctcggcgta gattccgctg aagaaaccgg cctgaccttt atcgaaaacg
   114241 cgattctgaa agcgcgccat gcggcaaaag tgaccggttt accggcaatt gccgacgact
   114301 ctggtctggc ggtagatgta cttggcggcg cgccgggcat ttactccgcg cgttattccg
   114361 gtgaagacgc gaccgatcaa aagaatctgc aaaaactgct ggaaacacta aaagacgtac
   114421 cggacaacca acgtcaggcg cgtttccact gcgtactggt atatctgcgt cacgcggaag
   114481 atcccactcc gctggtgtgc cacggcagct ggccgggcat gattactcgt gaaccagcgg
   114541 gtactggcgg ctttggttat gatccaatct tcttcgtacc ttccgaaggg aaaaccgctg
   114601 ccgaactgac ccgcgaagaa aagagcgcca tttcccaccg tggtcaggca ttgaaactgc
   114661 tgctggacgc tttacgtaat ggttaaatta ccgccgctaa gtctctacat tcacatcccg
   114721 tggtgcgtgc agaaatgccc gtactgcgat ttcaactctc acgcgttgaa aggagaagtg
   114781 ccgcacgacg attatgttca gcatctgctt aacgacctgg acaacgatgt ggcttacgct
   114841 cagggccgtg aagtaaagac aatctttatt ggcggtggta cgccgagcct gctttccggc
   114901 ccggcgatgc aaacgctgct ggacggcgtg cgtgcgcgtt tgccgctggc agcggatgca
   114961 gaaattacta tggaagcgaa cccaggcacg gtagaagcgg atcgctttgt cgattatcag
   115021 cgtgctggtg tgaaccgcat ctctattggt gtgcagagtt ttagcgaaga aaagctgaaa
   115081 cgacttgggc gtattcatgg cccgcaagaa gcgaaacgag cggcaaagct ggcgagcggt
   115141 ttagggttac gtagctttaa ccttgatttg atgcatggac taccggatca atcactggaa
   115201 gaggcgcttg gcgatctacg ccaggccatt gaactgaatc cgccgcatct ttcctggtat
   115261 caactgacca tcgaacccaa tacgctgttt ggttcgcgac caccggtgct gccggacgac
   115321 gatgcgttgt gggatatttt cgaacagggg catcagttat taaccgcagc gggttatcag
   115381 caatatgaaa cttccgctta cgccaaaccc ggttatcagt gccagcacaa tctcaactac
   115441 tggcgctttg gcgactacat cggtattggc tgcggcgcgc acgggaaagt gaccttcccg
   115501 gatgggcgca ttctgcgtac caccaaaacg cgtcatccgc gtggttttat gcaaggaagg
   115561 tatctggaaa gccagcgtga tgtcgaagcc gcagataagc cgtttgagtt ctttatgaat
   115621 cgcttccgtc tgctggaggc cgcgccgcgc gtggagttta ttgcgtatac cgggctttgc
   115681 gaagatgtga ttcgcccaca gttagacgag gcgattgccc agggttatct cacagaatgt
   115741 gcggattact ggcagataac ggaacatggt aaattattct taaactcact tcttgaatta
   115801 ttcctcgctg agtaatcatt aatg
//
LOCUS       DAZFFI010000004       100569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFFI010000004 DAZFFI010000000
VERSION     DAZFFI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.004"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..706)
                     /gene="gmhB"
                     /locus_tag="ACHJ12_000361"
     CDS             complement(131..706)
                     /gene="gmhB"
                     /locus_tag="ACHJ12_000361"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4988208.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            894..1925
                     /gene="metN"
                     /locus_tag="ACHJ12_000362"
     CDS             894..1925
                     /gene="metN"
                     /locus_tag="ACHJ12_000362"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4988209.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1918..2571
                     /gene="metI"
                     /locus_tag="ACHJ12_000363"
     CDS             1918..2571
                     /gene="metI"
                     /locus_tag="ACHJ12_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4988210.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2611..3426
                     /gene="metQ"
                     /locus_tag="ACHJ12_000364"
     CDS             2611..3426
                     /gene="metQ"
                     /locus_tag="ACHJ12_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4988211.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3544..3948
                     /gene="rcsF"
                     /locus_tag="ACHJ12_000365"
     CDS             3544..3948
                     /gene="rcsF"
                     /locus_tag="ACHJ12_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4988212.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            3945..4652
                     /gene="tsaA"
                     /locus_tag="ACHJ12_000366"
     CDS             3945..4652
                     /gene="tsaA"
                     /locus_tag="ACHJ12_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4988213.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            4763..6481
                     /gene="proS"
                     /locus_tag="ACHJ12_000367"
     CDS             4763..6481
                     /gene="proS"
                     /locus_tag="ACHJ12_000367"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4988214.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6534..7358
                     /gene="yaeF"
                     /locus_tag="ACHJ12_000368"
     CDS             6534..7358
                     /gene="yaeF"
                     /locus_tag="ACHJ12_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4988215.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7513..8223)
                     /gene="nlpE"
                     /locus_tag="ACHJ12_000369"
                     /gene_synonym="cutF"
     CDS             complement(7513..8223)
                     /gene="nlpE"
                     /locus_tag="ACHJ12_000369"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4988216.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8237..8659)
                     /gene="arfB"
                     /locus_tag="ACHJ12_000370"
     CDS             complement(8237..8659)
                     /gene="arfB"
                     /locus_tag="ACHJ12_000370"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4988217.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(8656..9201)
                     /gene="yaeQ"
                     /locus_tag="ACHJ12_000371"
     CDS             complement(8656..9201)
                     /gene="yaeQ"
                     /locus_tag="ACHJ12_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4988218.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9367..9567
                     /gene="yaeP"
                     /locus_tag="ACHJ12_000372"
     CDS             9367..9567
                     /gene="yaeP"
                     /locus_tag="ACHJ12_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4988219.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9554..9814
                     /gene="rof"
                     /locus_tag="ACHJ12_000373"
     CDS             9554..9814
                     /gene="rof"
                     /locus_tag="ACHJ12_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4988220.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9863..11161)
                     /gene="tilS"
                     /locus_tag="ACHJ12_000374"
     CDS             complement(9863..11161)
                     /gene="tilS"
                     /locus_tag="ACHJ12_000374"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4988221.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(11226..11615)
                     /gene="yaeR"
                     /locus_tag="ACHJ12_000375"
     CDS             complement(11226..11615)
                     /gene="yaeR"
                     /locus_tag="ACHJ12_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4988222.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11672..13813)
                     /gene="ldcC"
                     /locus_tag="ACHJ12_000376"
     CDS             complement(11672..13813)
                     /gene="ldcC"
                     /locus_tag="ACHJ12_000376"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4988223.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYAMGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13912..14871)
                     /gene="accA"
                     /locus_tag="ACHJ12_000377"
     CDS             complement(13912..14871)
                     /gene="accA"
                     /locus_tag="ACHJ12_000377"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4988224.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14884..18366)
                     /gene="dnaE"
                     /locus_tag="ACHJ12_000378"
     CDS             complement(14884..18366)
                     /gene="dnaE"
                     /locus_tag="ACHJ12_000378"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4988225.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18403..18999)
                     /gene="rnhB"
                     /locus_tag="ACHJ12_000379"
     CDS             complement(18403..18999)
                     /gene="rnhB"
                     /locus_tag="ACHJ12_000379"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4988226.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(18996..20144)
                     /gene="lpxB"
                     /locus_tag="ACHJ12_000380"
     CDS             complement(18996..20144)
                     /gene="lpxB"
                     /locus_tag="ACHJ12_000380"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4988227.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20144..20932)
                     /gene="lpxA"
                     /locus_tag="ACHJ12_000381"
     CDS             complement(20144..20932)
                     /gene="lpxA"
                     /locus_tag="ACHJ12_000381"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4988228.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20936..21391)
                     /gene="fabZ"
                     /locus_tag="ACHJ12_000382"
     CDS             complement(20936..21391)
                     /gene="fabZ"
                     /locus_tag="ACHJ12_000382"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4988229.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21496..22521)
                     /gene="lpxD"
                     /locus_tag="ACHJ12_000383"
     CDS             complement(21496..22521)
                     /gene="lpxD"
                     /locus_tag="ACHJ12_000383"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4988230.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(22525..23010)
                     /gene="skp"
                     /locus_tag="ACHJ12_000384"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22525..23010)
                     /gene="skp"
                     /locus_tag="ACHJ12_000384"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4988231.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23132..25564)
                     /gene="bamA"
                     /locus_tag="ACHJ12_000385"
     CDS             complement(23132..25564)
                     /gene="bamA"
                     /locus_tag="ACHJ12_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4988232.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25594..26946)
                     /gene="rseP"
                     /locus_tag="ACHJ12_000386"
     CDS             complement(25594..26946)
                     /gene="rseP"
                     /locus_tag="ACHJ12_000386"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4988233.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26958..27815)
                     /gene="cdsA"
                     /locus_tag="ACHJ12_000387"
     CDS             complement(26958..27815)
                     /gene="cdsA"
                     /locus_tag="ACHJ12_000387"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4988234.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27828..28586)
                     /gene="ispU"
                     /locus_tag="ACHJ12_000388"
                     /gene_synonym="uppS"
     CDS             complement(27828..28586)
                     /gene="ispU"
                     /locus_tag="ACHJ12_000388"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4988235.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28775..29971)
                     /gene="ispC"
                     /locus_tag="ACHJ12_000389"
     CDS             complement(28775..29971)
                     /gene="ispC"
                     /locus_tag="ACHJ12_000389"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4988236.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(30063..30620)
                     /gene="frr"
                     /locus_tag="ACHJ12_000390"
     CDS             complement(30063..30620)
                     /gene="frr"
                     /locus_tag="ACHJ12_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4988237.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30912..31637)
                     /gene="pyrH"
                     /locus_tag="ACHJ12_000391"
     CDS             complement(30912..31637)
                     /gene="pyrH"
                     /locus_tag="ACHJ12_000391"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4988238.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31784..32635)
                     /gene="tsf"
                     /locus_tag="ACHJ12_000392"
     CDS             complement(31784..32635)
                     /gene="tsf"
                     /locus_tag="ACHJ12_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4988239.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32893..33618)
                     /gene="rpsB"
                     /locus_tag="ACHJ12_000393"
     CDS             complement(32893..33618)
                     /gene="rpsB"
                     /locus_tag="ACHJ12_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4988240.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33986..34780
                     /gene="map"
                     /locus_tag="ACHJ12_000394"
     CDS             33986..34780
                     /gene="map"
                     /locus_tag="ACHJ12_000394"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4988241.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34842..37514
                     /gene="glnD"
                     /locus_tag="ACHJ12_000395"
     CDS             34842..37514
                     /gene="glnD"
                     /locus_tag="ACHJ12_000395"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4988242.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37545..38369
                     /gene="dapD"
                     /locus_tag="ACHJ12_000396"
     CDS             37545..38369
                     /gene="dapD"
                     /locus_tag="ACHJ12_000396"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4988243.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38684..39070
                     /gene="yaeH"
                     /locus_tag="ACHJ12_000397"
     CDS             38684..39070
                     /gene="yaeH"
                     /locus_tag="ACHJ12_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4988244.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39159..40316)
                     /gene="cdaR"
                     /locus_tag="ACHJ12_000398"
     CDS             complement(39159..40316)
                     /gene="cdaR"
                     /locus_tag="ACHJ12_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4988245.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40471..41895)
                     /gene="degP"
                     /locus_tag="ACHJ12_000399"
     CDS             complement(40471..41895)
                     /gene="degP"
                     /locus_tag="ACHJ12_000399"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4988246.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(42025..43542)
                     /gene="dgt"
                     /locus_tag="ACHJ12_000400"
     CDS             complement(42025..43542)
                     /gene="dgt"
                     /locus_tag="ACHJ12_000400"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4988247.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43626..44324
                     /gene="mtnN"
                     /locus_tag="ACHJ12_000401"
     CDS             43626..44324
                     /gene="mtnN"
                     /locus_tag="ACHJ12_000401"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4988248.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44317..45117
                     /gene="btuF"
                     /locus_tag="ACHJ12_000402"
     CDS             44317..45117
                     /gene="btuF"
                     /locus_tag="ACHJ12_000402"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4988249.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45155..45778
                     /gene="yadS"
                     /locus_tag="ACHJ12_000403"
     CDS             45155..45778
                     /gene="yadS"
                     /locus_tag="ACHJ12_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4988250.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45825..46169)
                     /gene="erpA"
                     /locus_tag="ACHJ12_000404"
     CDS             complement(45825..46169)
                     /gene="erpA"
                     /locus_tag="ACHJ12_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4988251.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46162..46227)
                     /gene="yadW"
                     /locus_tag="ACHJ12_000405"
     CDS             complement(46162..46227)
                     /gene="yadW"
                     /locus_tag="ACHJ12_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4988252.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46251..47672)
                     /gene="clcA"
                     /locus_tag="ACHJ12_000406"
     CDS             complement(46251..47672)
                     /gene="clcA"
                     /locus_tag="ACHJ12_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4988253.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47684..47731)
                     /gene="yadX"
                     /locus_tag="ACHJ12_000407"
     CDS             complement(47684..47731)
                     /gene="yadX"
                     /locus_tag="ACHJ12_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4988254.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47897..49177
                     /gene="hemL"
                     /locus_tag="ACHJ12_000408"
     CDS             47897..49177
                     /gene="hemL"
                     /locus_tag="ACHJ12_000408"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4988255.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49212..51194)
                     /gene="fhuB"
                     /locus_tag="ACHJ12_000409"
     CDS             complement(49212..51194)
                     /gene="fhuB"
                     /locus_tag="ACHJ12_000409"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4988256.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51191..52081)
                     /gene="fhuD"
                     /locus_tag="ACHJ12_000410"
     CDS             complement(51191..52081)
                     /gene="fhuD"
                     /locus_tag="ACHJ12_000410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4988257.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(52081..52878)
                     /gene="fhuC"
                     /locus_tag="ACHJ12_000411"
     CDS             complement(52081..52878)
                     /gene="fhuC"
                     /locus_tag="ACHJ12_000411"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4988258.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52929..55172)
                     /gene="fhuA"
                     /locus_tag="ACHJ12_000412"
     CDS             complement(52929..55172)
                     /gene="fhuA"
                     /locus_tag="ACHJ12_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4988259.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55392..57926)
                     /gene="mrcB"
                     /locus_tag="ACHJ12_000413"
     CDS             complement(55392..57926)
                     /gene="mrcB"
                     /locus_tag="ACHJ12_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4988260.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58122..60551)
                     /gene="hrpB"
                     /locus_tag="ACHJ12_000414"
     CDS             complement(58122..60551)
                     /gene="hrpB"
                     /locus_tag="ACHJ12_000414"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4988261.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDGLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60625..61155
                     /gene="thpR"
                     /locus_tag="ACHJ12_000415"
     CDS             60625..61155
                     /gene="thpR"
                     /locus_tag="ACHJ12_000415"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4988262.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            61170..61874
                     /gene="sfsA"
                     /locus_tag="ACHJ12_000416"
     CDS             61170..61874
                     /gene="sfsA"
                     /locus_tag="ACHJ12_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4988263.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            62052..62507
                     /gene="dksA"
                     /locus_tag="ACHJ12_000417"
     CDS             62052..62507
                     /gene="dksA"
                     /locus_tag="ACHJ12_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4988264.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62574..63470
                     /gene="gluQRS"
                     /locus_tag="ACHJ12_000418"
     CDS             62574..63470
                     /gene="gluQRS"
                     /locus_tag="ACHJ12_000418"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4988265.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63530..64927
                     /gene="pcnB"
                     /locus_tag="ACHJ12_000419"
     CDS             63530..64927
                     /gene="pcnB"
                     /locus_tag="ACHJ12_000419"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4988266.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64924..65403
                     /gene="folK"
                     /locus_tag="ACHJ12_000420"
     CDS             64924..65403
                     /gene="folK"
                     /locus_tag="ACHJ12_000420"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4988267.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            65773..66357
                     /gene="yadN"
                     /locus_tag="ACHJ12_000421"
     CDS             65773..66357
                     /gene="yadN"
                     /locus_tag="ACHJ12_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4988268.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            66462..67202
                     /gene="yadV"
                     /locus_tag="ACHJ12_000422"
     CDS             66462..67202
                     /gene="yadV"
                     /locus_tag="ACHJ12_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4988269.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            67237..69837
                     /gene="htrE"
                     /locus_tag="ACHJ12_000423"
     CDS             67237..69837
                     /gene="htrE"
                     /locus_tag="ACHJ12_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO4988270.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            69854..70423
                     /gene="yadM"
                     /locus_tag="ACHJ12_000424"
     CDS             69854..70423
                     /gene="yadM"
                     /locus_tag="ACHJ12_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4988271.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            70438..71040
                     /gene="yadL"
                     /locus_tag="ACHJ12_000425"
     CDS             70438..71040
                     /gene="yadL"
                     /locus_tag="ACHJ12_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO4988272.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            71067..71663
                     /locus_tag="ACHJ12_000426"
     CDS             71067..71663
                     /locus_tag="ACHJ12_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4988273.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            71647..72990
                     /locus_tag="ACHJ12_000427"
     CDS             71647..72990
                     /locus_tag="ACHJ12_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO4988274.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            73103..73897
                     /gene="panB"
                     /locus_tag="ACHJ12_000428"
     CDS             73103..73897
                     /gene="panB"
                     /locus_tag="ACHJ12_000428"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4988275.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73909..74760
                     /gene="panC"
                     /locus_tag="ACHJ12_000429"
     CDS             73909..74760
                     /gene="panC"
                     /locus_tag="ACHJ12_000429"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4988276.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74834..75736)
                     /gene="rpnC"
                     /locus_tag="ACHJ12_000430"
     CDS             complement(74834..75736)
                     /gene="rpnC"
                     /locus_tag="ACHJ12_000430"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4988277.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSRKDVAEMTNLSLAEIDKVINLI"
     gene            76010..76390
                     /gene="panD"
                     /locus_tag="ACHJ12_000431"
     CDS             76010..76390
                     /gene="panD"
                     /locus_tag="ACHJ12_000431"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4988278.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76394..77623)
                     /gene="yadE"
                     /locus_tag="ACHJ12_000432"
     CDS             complement(76394..77623)
                     /gene="yadE"
                     /locus_tag="ACHJ12_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4988279.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77687..78127)
                     /gene="yadI"
                     /locus_tag="ACHJ12_000433"
     CDS             complement(77687..78127)
                     /gene="yadI"
                     /locus_tag="ACHJ12_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4988280.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(78232..79002)
                     /gene="yadH"
                     /locus_tag="ACHJ12_000434"
     CDS             complement(78232..79002)
                     /gene="yadH"
                     /locus_tag="ACHJ12_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4988281.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78999..79925)
                     /gene="yadG"
                     /locus_tag="ACHJ12_000435"
     CDS             complement(78999..79925)
                     /gene="yadG"
                     /locus_tag="ACHJ12_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4988282.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            80034..80696
                     /gene="can"
                     /locus_tag="ACHJ12_000436"
     CDS             80034..80696
                     /gene="can"
                     /locus_tag="ACHJ12_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4988283.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80737..81273)
                     /gene="hpt"
                     /locus_tag="ACHJ12_000437"
     CDS             complement(80737..81273)
                     /gene="hpt"
                     /locus_tag="ACHJ12_000437"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4988284.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81479..83869
                     /gene="gcd"
                     /locus_tag="ACHJ12_000438"
     CDS             81479..83869
                     /gene="gcd"
                     /locus_tag="ACHJ12_000438"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4988285.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83916..85466)
                     /gene="cueO"
                     /locus_tag="ACHJ12_000439"
     CDS             complement(83916..85466)
                     /gene="cueO"
                     /locus_tag="ACHJ12_000439"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4988286.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85632..85979
                     /gene="yacC"
                     /locus_tag="ACHJ12_000440"
     CDS             85632..85979
                     /gene="yacC"
                     /locus_tag="ACHJ12_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4988287.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86085..86951
                     /gene="speE"
                     /locus_tag="ACHJ12_000441"
     CDS             86085..86951
                     /gene="speE"
                     /locus_tag="ACHJ12_000441"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4988288.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTEHDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            86967..87761
                     /gene="speD"
                     /locus_tag="ACHJ12_000442"
     CDS             86967..87761
                     /gene="speD"
                     /locus_tag="ACHJ12_000442"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4988289.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87799..88161)
                     /gene="yacL"
                     /locus_tag="ACHJ12_000443"
     CDS             complement(87799..88161)
                     /gene="yacL"
                     /locus_tag="ACHJ12_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4988290.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88336..90933)
                     /gene="acnB"
                     /locus_tag="ACHJ12_000444"
     CDS             complement(88336..90933)
                     /gene="acnB"
                     /locus_tag="ACHJ12_000444"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4988291.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91288..93045
                     /gene="yacH"
                     /locus_tag="ACHJ12_000445"
     CDS             91288..93045
                     /gene="yacH"
                     /locus_tag="ACHJ12_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4988292.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(93116..94540)
                     /gene="lpdA"
                     /locus_tag="ACHJ12_000446"
     CDS             complement(93116..94540)
                     /gene="lpdA"
                     /locus_tag="ACHJ12_000446"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4988293.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94865..96757)
                     /gene="aceF"
                     /locus_tag="ACHJ12_000447"
     CDS             complement(94865..96757)
                     /gene="aceF"
                     /locus_tag="ACHJ12_000447"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4988294.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96772..99435)
                     /gene="aceE"
                     /locus_tag="ACHJ12_000448"
     CDS             complement(96772..99435)
                     /gene="aceE"
                     /locus_tag="ACHJ12_000448"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4988295.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99596..100360)
                     /gene="pdhR"
                     /locus_tag="ACHJ12_000449"
     CDS             complement(99596..100360)
                     /gene="pdhR"
                     /locus_tag="ACHJ12_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4988296.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24499 a        27138 c        24715 g        24217 t
ORIGIN      
        1 ctctttaacc gaaccggctg tttgtgtgaa gtgattcaca tccgccgtgt cgatggaggc
       61 gcattatagg gagtcggctc aggaagacaa gcggaaaaat gcatttttat ttcaaccgct
      121 catcttttaa tcattgcgcc ggtttttgct gcttttttat cgcttgcggc aggtctgcca
      181 ggctatttaa cacccaatcc gccgcgtttt ctgcttcagg cgtaataggt ttacccgtac
      241 gcaccagcac ttttgttccc acgttcgccg cagccgctgc ctgcatatct tctaatttat
      301 cgcccaccat ataagaagcg gccatatcaa tatgcaaata atcgcgtgct gacaaaagca
      361 tccccggatg tggtttgcgg cagtcgcaga cctggcgaaa ctcttcaaca ctaccctgcg
      421 gatgatgcgg gcaataatag ataccatcca gatcgacatc gcggtccgcc agcgaccagt
      481 ccatccactc ggtcagcgtt tcaaactgtg cttcagtaaa tttaccgcga gcaatgccag
      541 actggttggt tactaccacc agcgcaaagc ccattttttt tagctcgcgc atggcgtcaa
      601 taacaccgtc gataaattca aagttgtcga tctcatggac atagccgtga tcgacattaa
      661 tggtgccatc acggtcaaga aaaattgcgg gtacgctctt cgccaccttt tatagctcct
      721 taataaggca tgtgacgcta gtatcgcatg tttcgacctg caagaaagtg ctcttcgcat
      781 aaacccgatt gatttagacg tctggatgcc ttaacatcca tttcattgac ggcgttgccc
      841 gtttcaggca ttcgagatgc cacgactaac ttaatgacga taataaataa tcaatgataa
      901 aactttcgaa tatcaccaaa gtgttccacc agggcacccg caccatccag gcgttgaaca
      961 acgtcagcct gcatgtgcca gctggacaaa tttatggcgt tatcggtgcc tcaggcgcgg
     1021 gtaagagtac gcttatacgt tgtgtaaacc tgctggagcg cccaaccgag ggtagcgtgc
     1081 tggtcgatgg ccaggaactg accacgctgt cagaatccga gttgaccaaa gctcgccgcc
     1141 agattggtat gattttccag cattttaacc tgctctcttc gcgtactgtt tttggcaacg
     1201 tggctctgcc gctggagctg gacaacacac cgaaagacga gatcaaacgt cgcgtgacgg
     1261 aattgctgtc attggttggt cttggcgata agcatgatag ctacccgtcg aatctttccg
     1321 gtgggcagaa acaacgtgtg gcgattgccc gtgcattagc cagcaatccc aaagtattgc
     1381 tgtgtgatga agccaccagc gcgctggacc cggcaacgac acgttctatt ctcgaactgc
     1441 tgaaagacat caaccgccgt ctgggtttga cgattctgtt gatcacccac gaaatggacg
     1501 ttgtaaagcg catttgtgat tgcgtggcgg tcatcagcaa tggcgaactg atcgagcagg
     1561 acacggtaag tgaagtgttt tcgcatccga aaacgccgct ggcgcagaag tttattcagt
     1621 cgaccctgca tctggatatc ccggaagatt atcaggaacg tctgcaagcg gagccattta
     1681 ctgactgcgt gccgatgttg cgtctggagt ttaccggtca atcggtcgat gccccactgc
     1741 tttctgaaac cgcgcgtcgt ttcaacgtca acaacaacat tattagcgcg cagatggact
     1801 acgccggtgg cgttaagttc ggcatcatgc tgactgaaat gcacggcaca caacaagata
     1861 cgcaagccgc cattgcctgg ctgcaggaac accatgtaaa agtagaggta ctgggttatg
     1921 tctgagccga tgatgtggct gctggttcgt ggcgtatggg aaacgctggc aatgaccttc
     1981 gtatccggat tttttggctt tgtgattggt ctgccggtcg gcgtcctgct ttatgttacg
     2041 cgcccggggc aaattattgc taacgcgaag ctgtatcgta ccgtttctgc gattgtgaac
     2101 attttccgtt ccatcccgtt cattatcttg ctggtatgga tgattccgtt tacccgcgtt
     2161 attgtcggta catcgattgg tttgcaggca gcgattgttc cgttaaccgt tggtgcagca
     2221 ccgtttattg cccgtatggt cgagaacgct ctgctggaga tcccaaccgg gttaattgaa
     2281 gcttcccgcg caatgggtgc cacgccgatg cagatcgtcc gtaaggtgct gttaccggaa
     2341 gcactgccgg gtctggtgaa tgcggcaact atcaccctga ttaccctggt cggctattcc
     2401 gcgatgggtg gtgcagtcgg tgccggtggt ttaggtcaga ttggctatca gtatggctac
     2461 atcggctata acgcgacggt gatgaatacg gtactggtat tgctggtcat tctggtttat
     2521 ttaattcagt tcgcaggcga ccgcatcgtc cgggctgtca ctcgcaagta acgttcaaca
     2581 caacataaat aattgaagaa ggaataaggt atggcgttca aattcaaaac ctttgcggca
     2641 gtgggagccc tgattggatc actggcactg gtaggctgcg gtcaggatga aaaagatcca
     2701 aaccacatta aagtcggcgt gattgttggt gccgaacagc aggtagcaga agtcgcgcag
     2761 aaagttgcga aagacaaata tggcctggac gttgagctgg taaccttcaa cgactatgtt
     2821 ctgccaaacg aagcattgag caaaggcgat atcgacgcca acgccttcca gcataaaccg
     2881 taccttgatc agcaactgaa agatcgtggc tacaaactgg tcgcagtagg caacactttt
     2941 gtttatccga ttgctggtta ctccaagaaa atcaaatcac tggatgaact gcaggatggt
     3001 tcgcaggttg ccgtgccaaa cgatccaact aatcttggtc gttcactgct gctgctgcaa
     3061 aaagtgggct tgatcaaact gaaagatggc gttggcctgc tgccgaccgt tcttgatgtt
     3121 gttgagaacc caaaaaatct gaaaattgtt gagctggaag caccgcagct gcctcgatct
     3181 ctggacgacg cgcaaatcgc tctggcagtt atcaatacca cctatgccag ccagattggc
     3241 ctgactccag cgaaagacgg tatctttgtc gaagataaag actccccgta cgtaaacctg
     3301 atcgtaacgc gtgaagataa caaagacgcc gagaacgtga agaaattcgt ccaggcttat
     3361 cagtctgatg aagtttacga agcagcaaac aaagtgttta acggcggcgc tgttaaaggc
     3421 tggtaatttt taggctgttt ccacaatttg taatatcatt caggacgggc gcttgcccgt
     3481 cttgtcattt ttacaagctc ctgattcaat attgacgttt tgatcataca ttgaggaaat
     3541 actatgcgtg ctttaccgat ctgtttagta gcactcatgc taagcggctg ttccatgtta
     3601 agcagatccc ctgtcgaacc cgttcaaagc actgcacccc agccgaaagc ggagcctgca
     3661 aaaccgaaag cgccgcgcgc cacgccggtc cgaatttata ccaatgcaga agaattagtc
     3721 ggcaaaccgt tccgcgatct cggtgaagtc agtggcgatt cttgccaggc ctccaatcag
     3781 gactctccgc cgagcattcc aaccgcacgt aagcggatgc aaatcaacgc ctcgaaaatg
     3841 aaggccaatg cggtattact gcatagctgc gaagtcacca gcggtacgcc aggctgctat
     3901 cgtcaggctg tatgtatcgg ttctgcgctt aacattacgg cgaaatgagc agtttccagt
     3961 tcgagcaaat aggcgttatt cgctcgccct ataaagaaaa attcgccgtt ccgcgccagc
     4021 caggtctggt aaaaagcgcc aacggcgaac tgcatctcat tgctccctac aaccaggccg
     4081 acgccgttcg cggcctggaa gcgttcagcc atttgtggat ccttttcgtc tttcatcaaa
     4141 cgatggaagg cggctggcgt ccgactgtgc gtccaccgcg cctcggcggt aacgccagaa
     4201 tgggggtttt cgcaacacgc tctactttcc gccctaaccc aattggtatg tcgctggtag
     4261 agctgaaaga ggttgtttgc cataaagaca gcgtgattct gaagctcggc agtctggatc
     4321 tggtcgatgg tacgccggta gtggatatca aaccatatct cccttttgcc gaatcgcttc
     4381 ccgatgccag tgccagctat gcgcaaagtg cgccagcggc agagatggcg gtgagtttta
     4441 ccgcagaggt cgaaaagcag cttttgacgc tggagaagcg ttatccgcag ttaacgctgt
     4501 ttatccgcga agtactggcg caggacccgc gcccggccta tcgtaaaggt gaggaaacgg
     4561 gcaaaaccta tgccgtctgg ctacatgatt ttaacgttcg ctggcgcgtc accgacgcag
     4621 gttttgaagt ctttgcgctg gaaccgcgtt aaattcacgc ccttctcttt tgacatttct
     4681 tttgcactgg taaactaaat cacttttttt gtcccaggct cgccttgagc ctgttctacc
     4741 ttccaactgg aaccgtaaca acatgcgtac tagccaatac ctgctctcca ctctcaagga
     4801 gacacctgcc gacgccgagg tgatcagcca tcagctgatg ctgcgcgccg ggatgatccg
     4861 caagctggcc tccgggttat atacctggct gccgaccggc gtgcgcgttc tgaaaaaagt
     4921 cgaaaacatc gtgcgtgaag agatgaacaa cgccggtgcg atcgaggtgt cgatgccggt
     4981 ggttcagcca gccgatttgt ggcaagagag tggtcgttgg gaacagtacg gtccggaact
     5041 gctgcgtttt gttgaccgtg gcgagcgtcc gttcgtactc ggcccaactc atgaagaagt
     5101 tatcactgac ctgattcgta acgagcttag ctcttacaaa cagctgccgc tgaacttcta
     5161 tcagatccag accaagttcc gcgacgaagt gcgtccgcgt ttcggcgtca tgcgttcccg
     5221 cgaattcctg atgaaagatg cttactcttt ccatacttct caggaatccc tgcaggaaac
     5281 ctacgatgca atgtatgcgg cctacagcaa aatcttcagc cgcatggggc tggatttccg
     5341 cgccgtacag gccgacaccg gttctatcgg cggcagcgcc tctcacgaat tccaggtgct
     5401 ggcgcagagc ggtgaagacg atgtggtctt ctccgacacc tctgactatg cagcgaacat
     5461 tgaactggca gaagctatcg cgccgaaaga accgcgcgct gctgctaccc aggaaatgac
     5521 gctggttgat acgccgaacg cgaaaaccat cgcggaactg gttgaacagt tcaatctgcc
     5581 gattgagaaa acggttaaga ctctgctggt taaagcggtt gaaggcagta gcttcccgct
     5641 ggttgcgctg ctggtgcgcg gtgaccacga actgaacgaa gttaaagcag aaaaactgcc
     5701 gcaggttgcc agcccgctga ctttcgcgac cgaagaagaa attcgtgccg tggttaacgc
     5761 cggtccgggt tcactgggtc cggtaaacat gccgattccg gtggtgattg accgtaccgt
     5821 tgcggcgatg agtgatttcg ctgctggtgc taacatcgat ggtaaacact acttcggcat
     5881 caactgggat cgcgatgtcg ctaccccgga aattgctgat atccgcaacg tggtggcggg
     5941 cgatccaagc ccggatggcc agggtacgct gctgatcaaa cgtggtatcg aagttggtca
     6001 catcttccag ctgggtacca agtactccga agcactgaaa gcctccgtac agggtgaaga
     6061 tggccgtaac caaatcctga cgatgggttg ctacggtatc ggggtaacgc gtgtggtagc
     6121 tgcggcgatt gagcagaact acgacgaacg cggcatcgta tggcctgacg ctatcgcgcc
     6181 gttccaggtg gcgattctgc cgatgaacat gcacaaatcc ttccgcgtac aagagcttgc
     6241 tgagaaactg tacagcgaac tgcgtgcaca aggtatcgaa gtgctgctgg atgaccgaaa
     6301 agaacgtccg ggcgtgatgt ttgccgatat ggaactgatc ggtattccgc acactattgt
     6361 gctgggcgac cgtaacctcg acaacgacga tatcgaatat aaatatcgtc gcaacggcga
     6421 gaaacagtta attaagactg gtgacatcgt cgaatatctg gtgaaacaga ttaaaggctg
     6481 atgccagaaa gggtcctgaa tttcagggcc cttttttaca tggattgtat aaaatggata
     6541 aaccaaaggc gtactgccgc ctgcttctcc cctgctttct tttactttcg gcctgtaccg
     6601 ttgatatcag ccagccagac tcctctgcca cagcggtaga tgtcgaggca aaaacatggg
     6661 ctgtcaaatt ccagcatcaa agctctttca ccgaacaatc aataaaagaa ataacggcac
     6721 ctgatctcaa acccggcgat ttgctgttct cctcaagcct tggggtaacc tcatttggaa
     6781 tccgcgtctt cagcacttcc tctgtgagtc acgttgcaat ctatctgggt gataataacg
     6841 ttgcagaagc gacaggcgct ggcgtccaga tcgtttccct taaaaaagcg atgaagcata
     6901 gtgataagct tttcgtctta cgagtcccgg atcttacccc gcaacaagcc acagatatca
     6961 ccgcgtttgc caataaaatc aaagatagcg gttataacta tcgcggcatt gtcgaattta
     7021 ttccctttat ggtgactcgc cagatgtgct cactgaatcc gttctccgag gattttcgcc
     7081 aacagtgcgt cagcggcctg gcgaaagcgc agttaagcag tgtgggcgaa ggagacaaaa
     7141 agtcgtggtt ttgttcggaa tttgtcactg atgcatttgc caaagccggg catccgctta
     7201 cactggcgca atcgggctgg attagccctg ccgatctgat gcatatgcgt attggtgatg
     7261 tctctgcgtt taagccagaa acgcagttac agtatgttgg gcacctgaag cctgggattt
     7321 atatcaaggc gggtcgcttt gttgggctga ctcagtgata aagccagcct cgtgcacaaa
     7381 atgccggatg cgtcgtgaac gccttatccg gcctacaaag tcctgcagat tcaatatatt
     7441 acaggtactg cgtagatact gataagcgca gcgcatcagg caattttgcg tttgtttctg
     7501 ccttaagcag gtttactgcc ccaaactact gcaatcctta ttcgggtaaa atttacccgc
     7561 cgtatcgggc gctaatactt tggttggcgc accagtatcc ggattagcct caagcgtaaa
     7621 gtgaccttct actgacagta acaccggttt ttcactgtga ccgcgcgcag ccaggtagct
     7681 acgctccagc tctgcgttat tcgctaccat gaaacgtttt ccggtcgcgc aatcagtgaa
     7741 ggtcgccgca tcagccatat aaaaatacat gcctcgcagg gtcatcggcg tcataggtaa
     7801 actagattgt gccggttcca gcgtatagtt gaactgcgat tcaatcggat tgccttcacg
     7861 atcgagcatc tccagcgcat cgcctttcgc ccgataatat gacttttcac ctttgctgtc
     7921 ggttaatacc agcttgtcag cggttcgcgc ccatgtaccg taggaagcga aggaggaagg
     7981 ttcttcacga gcaccgagat aacgctcatt catcacccat gttccgtctt tttcgaggaa
     8041 cagagaggtt tcgattcctt cgcaatcagc acacggcagc acgccgcgcc aactttgcgg
     8101 catcggtttc agttcggcag cctgtgctgg agaaagcgta tcgacttcgg cccggttatt
     8161 acatcccatc agagtaaaga ggctgattac agccatcgct gtcactatcg cttttttcac
     8221 catctattcc ttctttttat tcccgaccgc tgcgtacttt gccgcgcatc gccttcacgc
     8281 ttgatttttg tgctttcgat gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg
     8341 gtcgtcgggc tttttgttct gttgttaaat ctttaatcac tgccaccagc cgggctagcg
     8401 cggcttcgcg gttcagttcc tgactgcggt attcctgtgc cttaatgaca atcacgccat
     8461 cactgctgat caaatgatgg ctggcggcga gtagacgctc tttgtaatac tctggcaggc
     8521 tggacgcccg aatgtcaaaa cgcagatgaa tggccgttga ggtcttatta acatgctgcc
     8581 cgcccgcgcc ctgcgcacga atggcggtga tctcaagctc accatcggga atagcaacat
     8641 gtcgggaaat cacaatcatg aaggttgttg ccaggcggtt aagttaactt ccagattatt
     8701 cttatcgtcc gataaccaga tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg
     8761 atcggcaaag gcgcttactt tcgccagttg ttcatcgtcc agataccaga cggaaagatt
     8821 ggcaaactgc acacatttgc tctgattttg ctgccaccag atttgcgccg cccgactatt
     8881 ataggtaaac agcaccactt ctgcggcctg ggtgcaggct ttcttaatcc gccgctcatc
     8941 cggcagcccc agctcaatcc acaaatcaat gcccaggtga tcgttacgca gccacgcttc
     9001 cggctcatca tcggcacaca aaccacgggt aaattgcaga cgttcatcag catatttcag
     9061 ccacgccagc aagcgcagca tcatacgctc ctgggtttct gaagggtggc gcgccagcgt
     9121 cagagaggca tcgagaaact ggttgcggtc gagatcggcc acattaaccg tcgctttata
     9181 aattgtcgct ttaagcgcca taagaaaact cctttcgaat caggaggcca ttgtaacgga
     9241 atacgtgcag ttgcgacgct gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa
     9301 cagagtttat cttcgtaggc ttagacttgc atccacggtt aagtcagagt gctgacagga
     9361 gggcatgtgg aaaaatattg tgagttaata cgcaagcggt acgcggaaat cgccagcgga
     9421 gacttaggat acgttccgga cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct
     9481 gccgacgacg ccctttctga ggctgtcagg gaaaaagcag catacgctgc ggcaaactta
     9541 ctggtgagcg attatgtcaa tgaatgatac gtatcaacca atcaattgtg atgattacga
     9601 taatcttgag ctcgcctgcc agcatcattt aatgctgaca cttgagctga aagatggcga
     9661 aaaattgcag gcaaaagcca gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt
     9721 cgaggccgct ggcgaaaccc gtgagctacg tctggataaa attaccagct ttagccaccc
     9781 ggaaatcggt acggtggtgg tgagcgagtc ctgattgact ttcgccggat gcgacgtttg
     9841 atgcgtctta tccggctttc acttaactaa gcgttttctg ccagacaaaa cttacgccat
     9901 tctcaccttc agccacacct tcttgcgtca caaatacccc tgccgccgca atcagcgttt
     9961 cgccataaaa cagcagtggc gtggtgtcac gtagccacgg cggcacgcct aactcttgcc
    10021 agattttctt cagcttacgt ccgccgttgc gcccgacaat atgcagcaat cctgccgctt
    10081 tgaaacgcac gctgaccgct tcgtctgcac gcggagggcg aatatcgcct cccgcagtaa
    10141 gctgtacgct tcccagcccc gccggtaatt ccagcggttg tagccacgtc tgccacggca
    10201 caatggtttc actttgcccg gtgacggatt taatccacca cagttgcgac tgatagcggc
    10261 ggatttcaaa cgcgcccaag cgtaaacagg gggaggcatc ttcccgcgcc agcgccactt
    10321 cctgccagat ccgcaccagc gcgtcgcggg aaggcattgg tgcattctgc cctgccagcc
    10381 agcgacggat aatcgccgcg cggcgggcat cactcatcgc cagcattggc gcaatctgca
    10441 gcgtcccctg cgacgtttgg cagtgtgcta aatcatccgc cagcagttca tccaacagac
    10501 tctcttgttc agcacaaagt gcggcgctgc gggccgttgc ttcggcaaaa tgcggccaac
    10561 gctgctgcaa taacggcact acgcgcaggc gcagaaagtt acgatcgtat gagtcgtcct
    10621 gattactttc gtcttcaatc cagcgtaaac catgcgccaa tgcccactgc tccagttctc
    10681 cccgcgtgcg ggcgagcaac gggcggataa gccgtgttcc ggcaaactcc gaaacttccg
    10741 ccatagccga aagcccggca gggccgctac cgcgttttag cgccagcaga aaagtttcac
    10801 attgatcgtc gagatgttgc gcggtgacca gcacttcacc gggcaacaag gtgcgggcaa
    10861 atgcctgata acgcgcctgc cgcgcctggg cctcaatgcc cagtccttct tgcgcaagtt
    10921 gcacgcgttc gaccaccagc ggcacctgcc actgttggca gacgttttcg caatgcgtaa
    10981 cccaggcatc ggcattggca cttaaaccgt gatgcacatg aatagcgcgc agagtgacgc
    11041 ccggattttc cgtccgccac tgcaccaact gatgcagcag aacggtggag tcaagcccgc
    11101 cgctaaaggc caccagaatc tggcgtgagg tgagaagttg tctattgagc gtgagtgtca
    11161 tgatggtgcg attttacagt agcaatgccc ggcacgttac cgggcaaatg cggcgatgac
    11221 aagccttact gctcatacag ttccaacggc agcccgtccg gatcgttaaa gaaggtgaag
    11281 cgtttttgcg tgtatggatc gacacggatg gcttcacact tcacgttatg gctttcaagg
    11341 tgcgccactg ccgcatcgat gtcatcaacg ctaaaagcca gatgacgcag gccgcaagct
    11401 tccggtcgac tggggcgctc cggcgggaac ggaaaagaga aaagctcaat cacatattgc
    11461 ccattaagcg ccaaatcccc tttccacgag tcgcgcgctt cgcgatagac ttcgctttgc
    11521 agcgtgaaac caagaatatc gcagtagaaa gctttgctca ccgcataatc cgtcgcaata
    11581 atcgcaatat ggtgaacctg ttttaaaccc agcataacgt ctcctttatt tgttaacagc
    11641 acgttactcg cccggaagcc gctctggcaa gttatcccgc catttttagg actcgtacgc
    11701 ggtaaacgcc gtcttcgtcc tgtttcgcgc cgtgaatatc cgtttcaaaa ccggggtaat
    11761 gttgccctac ggaacaaagc atcagtagaa aatcgagtac cgtgcggctc tctttggtca
    11821 gcatttctcc tggcatcaac agcggtacgc ccggtggata aggcaggatc atatttgccg
    11881 atactctacc gaccagttgt tccagcgcaa tggtttctac ttcaccttta atctgtctct
    11941 gccatgcctg atgtggcgtc atgatcattt ccggcaaagt atcgaatgcc cgcaacatca
    12001 aaccgggaag atcgtgttta cgaatcagct tatggatccc ttgcgccaga tcctgaatac
    12061 gcatattgcg gtagaaatcg ggatcttcag catagagatc gggtagcata tttttgatcc
    12121 gcaggttgag atcgtaagag cgtttaaatt ccgtcaaccc acgcaataat cccattgctt
    12181 tggttttatc gataccaata ctaaagagaa acagcaggtt ataagggccg gttttctcta
    12241 ctacgatccc acgttcgtcg aggaattttg ccaccagcgc cgccgggatc ccctcctcgc
    12301 tcatattgcc ctgttcgtcc atccccggtg tcaaaatagt gactttaacc ggatcgagaa
    12361 acatatgatc ggcatccgca tcgttaaagc cgtgccactg ttcgcctggc gcaacgggcc
    12421 agcattcggc ttcatccacc tgcggcggtt gccagatatc gaaaaaccaa ccgtcagact
    12481 cttcccgcaa ccgctggacc tctttgcgaa aatgcagcgc ccgctccacc gaacggttaa
    12541 tcagccgttt gcccggatta ccacgcagca tcgccgccgc cgtctcaacc gaagcaacaa
    12601 tgggataact gggcgaggtg gtggtatgca tcataaaggc ttcgttaaac gcctcttcgt
    12661 catactcgcc tttaatgtgg atcagcgaag cctgcgataa cgccgccagc attttgtggg
    12721 tcgattgcgt ttcgaagatc acttttcccg caacacgctc gccgctcata ccacttttcc
    12781 cctggtagat cggatgaaaa tgggtgtacg gcacccaggc ggaatcgaag tggatcgacg
    12841 ggacatccag cgtctgtttg atccagtcgg tgttgtagag caagccatca taggtggagt
    12901 tggtaatcac cgcatggacc ggccattgtg cctgcgtggt ggcagcgact ttctcttcga
    12961 tgctgtcgcg agtaaattca cggcgcggga tcccaccaag aatccccaac gcattacgcg
    13021 tcggtttcag ccagactggc actacatcgt tcatcatcaa cagatgcgcc agcgatttat
    13081 gacaattgcg gtcgatcaac agcgtactgc cggatggcgc ggcgtacata cccacaattt
    13141 tgttcgacgt cgatgttccg ttggtaacga tataactctg ttccgcgcca aaagtccgcg
    13201 cgatgtactc ttccgcttcc aggtgtggcc cggtgtggtc gagcaacgaa ccaagctcgg
    13261 tgaccgaaat agagacatca gccttgaggg tattcccgcc gaaaaaatca taaaacaggc
    13321 agccaaccgg gcttttttga tatgcggtgc cgcccatatg ccccggcgta caaaaggtgt
    13381 acttccgctc tttgacgtag gtaaacaagg ctttcgtgaa cggcggtgta atgttatcaa
    13441 gatattcgtc ggtgtactga cgcatacgaa tggcgatatc ttccgcctgc cccatcgcat
    13501 attcaaaaaa ccagagcgcc atccgcatat cctgcacgct gacatccatc gtcgagtggg
    13561 tgttgatgaa ggcataaagc gggagatatt cattaagttg attgatatcg ctgcataaat
    13621 cgagactgta ctcatcccag tcaaaaatca cgccgcaaat tcgagggtta tgttcgataa
    13681 atttcagcaa atcaacgctg ttttgtggcc agataatctg aaagccttgc gccaccagcg
    13741 ccgactccag ttctttgatg ggctcatctt tataaaagac gccatgcggt cccataatgg
    13801 caatgatgtt catgtgttcc tcctggaaaa tccttcctta atcatagcct gctcaaaccg
    13861 tggcgataaa aaagggccac cgaagtgacc ctttttcaga acttttgcga attacgcgta
    13921 accgtagctc atcaggcgct gataacgacg attttttaaa tcttcagtgc ttaacacatc
    13981 gagatcggcc agatccgcca gcagttgcgc tttcaacgat gccgccatcg cttccgggtt
    14041 acggtgagcg ccgcccagcg gttccgggat gatggagtcg atcagtttca gctctttcag
    14101 acgcggagcg atgataccca tcgcttcagc cgccagcggc gctttgtcgg cgctcttcca
    14161 cagaatggac gcacaacctt ccggcgagat aacggaatag gtgctgtatt gcagcatatt
    14221 cactttatcg cccacgccaa tcgccagcgc accgccagaa ccaccttcac cgataaccgt
    14281 acaaactacc ggtacgccga ggcgagacat ttcacgcagg ttgcgtgcaa tggcttcaga
    14341 ctgaccacgc tcttctgcgc ccacgcccgg ataagccccc ggggtgtcga taaaggtgat
    14401 gattggcatc ttaaagcgtt cagccatttg catcagacgc agtgctttgc ggtaaccttc
    14461 tggcgctggc ataccaaagt tacggcgaat tttttctttg gtttcacgac ctttttgatg
    14521 accaatgatc atcaccggac gaccatcgag acgggcgata ccaccgacga tagctttatc
    14581 gtctgcatac gcgcggtcgc cagccagttc gtcaaattca tcaaatgcca ggcgaacgta
    14641 atccagggta taaggacgct gtggatggcg tgccagttgc gcaatctgcc atgcaccgag
    14701 atcggcgaag attttacgcg tcagttctac gcttttttca cgcagacgat gcacttcttc
    14761 atcgatgtta atatccagtt tctcatcctg acggctaacc gcagtcagag aatcgatttt
    14821 cgcttccagc tctgcaatcg gctgttcaaa atcaaggaaa ttcagactca tagtattcct
    14881 gtattagtca aactccagtt ccacctgctc cgaaccaatg aggccacgga gatcgtttaa
    14941 taaacgatcg ctcggagaga cacgccacgt cgcgccaaaa cgcaaccgcg cgcgtgcatc
    15001 cgccctctga tagtagagat gtactggaat tgtcccagag cggtggggtt ccagagactg
    15061 acggagtcgg tttaaaagct ggtcatcaat ttgcctgtcc gtcagcgaga tagcaagccc
    15121 gcgagcatat ttttcccggg cttcgtcaat atccatcact tcgcgagcgg tcattttaag
    15181 cccaccgctg aagtcatcaa agctgacctg tccgctgacg ataagtatgc ggtctttttc
    15241 cagcaattgc tggtatttat ccagggcgtc agtaaacaac atcacttcca gccgcccgga
    15301 acggtcatcc agcgtgcaga taccgatacg attgccgcgc ttggtgacca taacccgcgc
    15361 ggcaacaacg agccccgcag ccgtgatgac tttaccacgt tctgtcgggt gcatgtcttt
    15421 cagccttacg cctccgacat aacgctcaat ctcttttaaa tactggttga tagggtgtcc
    15481 ggtcaggtac aggcctaacg tttcacgttc cccatctaat accacctgct ccggccacgg
    15541 ttggcagctg gcgtaggatt gttcaatttg ttccggctct tcggccagca cgccgaacat
    15601 atcggcctga ccgatagctt ccgctttcgc gtgttgatct gccgctttta acgcatcgcc
    15661 cagcgagttc atcagtgccg cgcggtgtgg tccaagacgg tcaaacgccc cggacatgat
    15721 cagtttttcc agcacccgcc ggtttaactt tttggtgtcg gtacgggcgc aaagatcaaa
    15781 cagttcgcgg aagtagccgc ctttattacg ggcttcgatg atggcctcaa tcggaccttc
    15841 acctacccct ttgatggcgc caataccata cacgatttcg ccgtcgtcgt tgacgtggaa
    15901 atggtaaaga ccggagttta tatctggtgg caggattttc agccccatcc gccagcactc
    15961 atccaccagg cccaccacct tctcggtgtt gtccatatcg gcggtcatta ccgccgccat
    16021 aaactccgca ggatagtgtg ctttcagcca taacgtttga tatgacacca aagcataggc
    16081 cgcagagtgc gatttgttaa atccgtaacc cgcgaatttc tccaccaggt cgaagatttt
    16141 catcgccagt tcggcgttga ttccgttctt ttctgcacct tcagcaaata cagaacgctg
    16201 cttagccatc tcttccggct ttttcttacc catcgcacga cgcagcatat ccgcgccacc
    16261 gagggtataa ccagaaagca cctgggcaat ctgcatgacc tgttcctgat acaggatgat
    16321 gccgtaggtt ggctccagta ccggtttcaa gctttcatgc tgccactgca cgtccggata
    16381 ggagatctct tcacgaccat gtttacggtc gataaagtta tccaccatcc ctgattgcaa
    16441 cggaccgggg cggaacagtg ccaccagggc gatcatatct tcgaagcagt caggttgcag
    16501 acgcttgatc aggtccttca tgccgcgcga ttcaagctgg aataccgcag tggtttccga
    16561 gcgttgcagc atgtcgaagc ttttcttgtc atccagcggg atcgcggcga tatccagcgg
    16621 cggctcgcca ttcttcgccc gccgcttgtt gatcatctcc agcgcccagt tgatgatggt
    16681 gagcgtacgc aaaccaagga agtcgaactt caccagtccg gcgtattcaa cgtcgctttt
    16741 atcaaactgg gtgaccggat gtttgccctc ttcatcgcaa taaagcggcg caaaatcggt
    16801 aattttggtc ggtgcgataa ccaccccacc ggcgtgctta ccggcgttac gggtgacccc
    16861 ttccagtttg cgcgccatgt cgatcagcgc cttaacttct tcatccgctt cgtagatttc
    16921 cggcagctgc ggctcggctt caaacgcttt cgccagcgtc atccccggat cgggcgggat
    16981 cagtttcgag atacgatcga caaagccgta cggatgcccc agcacgcggc ctacgtcgcg
    17041 gatcaccgct ttcgccgcca tcgtaccgaa ggtgataatc tgcgataccg catcacgacc
    17101 gtacatgtcc gctacgtgct cgataacctg atcgcgtttc tccatacaga agtcaacgtc
    17161 gaagtcaggc atggagacac gttccgggtt aaggaaacgt tcgaacagca ggtcaaattc
    17221 cagcggatcg aggtcggtga ttttcagcgc gtaggccacc agtgaacccg caccggagcc
    17281 acggcctggc cctaccggta cgccgttatc tttcgaccac tggataaatt ccataacgat
    17341 gaggaagtag cccgggaagc ccatctggtt gataacctga agttcagtct ccagacgttc
    17401 gtcatattcc gggcggcgct taagacgttc ttcctcatca gggaataaaa aggccagacg
    17461 ctcttccagg ccctcttttg cacgcttgac cagataatct tcggtgctca tgtccccggt
    17521 cgggaactgc ggcaggaagt attcaccaag acgcacggtt acgttacagc gtttggcgat
    17581 ctcaacggtg ttggcaaggg cttcggggat gtcggcaaac agctcacaca tctcctcttc
    17641 gctacgcata tattgctgcg gcgaatagtt acgcgggcgt ttaggatcgt cgagggtaaa
    17701 gccgtcgtgg atcgcgacgc ggatttcgtg tgcgtcaaag tcgctgctgt cgataaagcg
    17761 cacgtcgttg gtcgccacga cgggcaaacc gcgcgcttcc gccagttcca ccgccgcgtg
    17821 cagatagctt tcttcatccg gcctgccggt gcggatcagc tcaagaaaat agcgatccgg
    17881 gaagtgttct tcataaaacg cgacacactc atctaccagc gcgctgttac cacgcaaaag
    17941 actgcgtccg acgtcgccca tgcgtccgcc ggaaagaagg atcaaccctt cgtttaattc
    18001 gataagccag tcgcgatcga tgatcggccc ggcggcaccg tacccgcgct gatacgcttt
    18061 tgagatcagc aacgtcagat tctgatagcc ggtattgttc gccgccagta ccgtcaggtg
    18121 ggttaactca tcacccagca ggtcgcactg gacgttaaaa tctgccccga cgataggctt
    18181 aatccctgcg ccatgtcccg ctccgtagaa cttcaccaga ccacaaaggt tggtgaaatc
    18241 ggtgatcgcc agtgctggca tacccaacgc cgccgccttt tttaccaacg gtgcggtttt
    18301 ggccaggcca tcgatcatcg agtagtcgct gtgcacccgc aggtgtacga aacgtggttc
    18361 agacatcttc agattccggt ttacttaatc tcgacacaag aatcaggacg caagtcccag
    18421 tgcgcgtttg acaggcccaa agctgcgccg atggtgttcg gtcgcgccgt gttcagccag
    18481 tttttccaga tgaaaagcgg ttgggtaccc tttgtgttgg gcaaaaccat attgcgggaa
    18541 aacaatatcc agcgccgcca tttcggcgtc acgcgtcact ttcgccagga tagacgcggc
    18601 actgatttcc ggtacgcggc tatcgccttt caccacagcc atcgcaggca tcggtaattt
    18661 cgggcagcgg ttaccatcaa tcaacacata ttccggcgca atatgcagcc cagcgacggc
    18721 acgctgcatc gccagcatgg tcgcatgaag aatgttcagc tcgtcgattt cgtggggttc
    18781 cgcgcggccc agactccagc tcaacgcttt ctctttgatc tcttcataga gcgccagacg
    18841 gcgtttttcg ctcagctttt tggaatcatt cagcccggca atcgggcgcg ccgggtcaag
    18901 gatcaccgca gcggtgacga ccgcgccaac taacggcccg cgtccgactt catccacacc
    18961 cgcaaccagc tgcgtgtgcg gataaacaaa ttcgatcatt gtgctaactc cagaacggct
    19021 tgtgccgcct gctcatcggc attgcagcgg atctgctgat gcagttcacg gaaggtatcg
    19081 tgcatcgcgt ggctggtttt cccgttcgcc aacagcggta acagcgccgc agccagtttt
    19141 tgcggctcac actcttcctg caataattct ttgactaact ctctgcccgc cagcagattt
    19201 ggcagcgaga cataatcagt tttcaccagc cgcttcgcca accagaaggt aaaaggcttc
    19261 atgcgatatc ccaccaccat cgggcatttc gccagcatac actccagggc tgccgtaccc
    19321 gacgccagta gcgccgcatc gctggcgacc atcgcctcac ggcccatccc atccagcaaa
    19381 tgaactgaaa ggtctggcgc gacttcagct ttgatgcgtt caaactgctc gcggcgtttg
    19441 gcattcacca gtggcaccac gatttcgaga tccggatatg tctggcgcaa aagctgggcc
    19501 gttttcagga aatcggcact aagcatttca acttctgcac cacggctccc tggtagcaac
    19561 gccaggcagt gggcatcgtg agggatcccc agcacatcac gggcggcatt tttatctgga
    19621 tctaacggca tggcatcagc catggtatga ccgataaagc ggcacggtac attgtatttg
    19681 tcataaaacg ctttttcgaa aggcagaaat gcgagcacca gatcggtggc tctgcctatt
    19741 ttgaaaacgc gtttctgtcg ccacgcccag acggatggac tgacgtaatg aatggttttg
    19801 ataccctgct ttttgaggtt accttcaaga gtaatattga agtcaggcgc atcaatacca
    19861 acaaaaatat ctggcttcag ttcgccaaaa cgctttgtca gatcggcacg aatatgcagt
    19921 aagcgacgca gacgaccgag cacttcaaca atgcccatca ccgccagttc ttccatttcg
    19981 taccaggctt cgcagccttc agcctgcatt cgtggcccgg caacaccaac aaagcgggcg
    20041 ttgggcacac gttctttcag agcgcggatt aaaccggccc ccaggatatc gccggaggtt
    20101 tctccggcga ccagggcaat cgttaatgga cgctgttcag tcattaacga atcagaccgc
    20161 gcgttgagcg tgcaaagaaa tcggtaaagg ctttcacttc cggatatgtt tccgccagtt
    20221 cagcaatttc cggtttcact tcatcgagcg ttttaccgct acgataaatc agcttatacg
    20281 cattgcggat agcggtaatc gcctcacggc tgaatccgcg gcgcttcagc ccttcgatat
    20341 tgacaccgaa cggcgttgcg tggttaccct gcgcaatgac ataaggaggg acgtcctgcg
    20401 ccacaccgga gcagccgcca accatcacgt gcgcaccaat gatgcagaac tgatggactg
    20461 cggtcatgcc gccgatgatc gcgaagtcgt caaccgatac gtgacccgcc agcgttgcgt
    20521 tgttggcgag aatacagcgg ttacctaccg tacaatcgtg cgcaatgtgc gcgttgatca
    20581 tcagtaagtt gtcgctgccc accttcgtca atccaccgcc ctggactgtg ccacgatgaa
    20641 tggtgacgct ttcgcgaatg cggttacgat cgccgatttc cacacgggtc ggttcgccag
    20701 catatttcag atcctggtta acttcgccga tggaggcgaa ctgataaatc tcattatcgc
    20761 ggccaatttt agtatgacca ttcacgacaa cgtgagattt cagtacggta ccctcaccaa
    20821 tttcgacatg gggtccaacg atacaaaaag gaccaatgtg tgcgttcgcg ccaattgacg
    20881 cgccctcttc cacaatggcg gttggatgca caaaggcgga tttatcaatc acttatcagg
    20941 cctcccggct acgagcacac atcatcgttg cttcgcaaac tactttacca tcgaccagag
    21001 caaccccttt aaaacgggtc aggccgcggc gcgttttttc gaaagtgact tccatgatca
    21061 tttgatcgcc aggcacgacc gggcgcttga agcgcgcttc gtcaatacca gcgaagtagt
    21121 acagctcacc cggttccagt tttcctacgc ttttaaacgc cagaatacct gttgcctgtg
    21181 ccattgcttc cagaatcagc acacccggga aaatcggttt tccagggaaa tggccctgga
    21241 agaatggctc attgacagag acatttttta ctgcgcgcag aaaacgacct tcttcaaaat
    21301 ccagcacgcg atccaccagt aagaacggga aacggtgcgg cagaagttct aaaatctctt
    21361 caatctgcag agtatgagtg ttagtagtca agatactctt cctgtcaaaa tataagaaac
    21421 gacaataata acacggcctg ccgcaatcgt aagaatgaga caggccgtaa agtttggcga
    21481 acaaaagatg gaacgttagt cttgttgatt aaccttgcgc tcaagcgatt tcagacgctt
    21541 gctcatgtca tcaatgttca tcaccagtgc agcggttttg cgccagactt tgttgggttg
    21601 cagcggaatg cctgaggaat agacgcctgg ttcagtgatg ggacgcatca ccatacccat
    21661 gcccgtaacc gtcactttgt cgcatatttc catatgcccg ttgattacgc tggctccgcc
    21721 gatcatgcag taacgaccaa ttttcaggct gcccgccata atgacgccac cggcaaccgc
    21781 cgtattgtcg ccaatcacga cgttatgtgc aatctggcac tggttatcaa tgatcacgcc
    21841 attgccaata atagtgtcat ccagcgcgcc gcgatcgatg gttgtgcagg caccgatctc
    21901 cacgcgatcg ccaataatta cgcgaccaat ctgtgggatc ttcacccagt taccacgatc
    21961 gttggcataa ccaaagccgt ctgcgcctac cactgttccg gactggatca ggcaattctg
    22021 accgatctgg atctcatggt aaatggttac gttcgcccag agacgcgaac ctgcaccgat
    22081 tttgctgttt ttacctacga agcaaccggc accgataatc acgttatcgc ccagttcaac
    22141 gccggactca atcaccgcgt tagcgccaat cgatacgttg ttacccagct tcgccgtcgc
    22201 gtcgatcacc gcactgggtg caatgttctg cgcgggctgc ggcgtggtat ctaaaatttg
    22261 cgccatgcgc gcgtaagtca ggtagggatt cttcactacc agcgcggcac ttttcgcgaa
    22321 aggaagatcg tcctgggtca tgacaaccgc ggacgcctgg cacaagccta aatgctcacg
    22381 gtattttggg ttaaccatga acgtaatgtg acctgtttgt gcagattgca tggacgcaac
    22441 gccggtgatg acgatatcgc catcaccgtg tagttctgca tccaactgct gcgctaaatc
    22501 agccagtcga attgaaggca ttacttattt aacctgtttc agtacgtcgg cagtgatgtc
    22561 ttttacatcg ctgctgttgt aagcaacggc gtttgcatca acaaccagat cgatatcctg
    22621 gctgttggca acggatttca cagcagtctg gatacgagta accagtttgc cgcgttcttc
    22681 gttggaacga cgtgcgcgat cctgctcaaa agcctgcgct ttctgagcaa aagtctggcg
    22741 ctgagccatc acgtcttttt ccagcttagt gcgatcgctg cccgctttca tggactgcag
    22801 ctttttcatt ttagcctgca gatcggtttc catacgctgc agttcgctgg cacggccttt
    22861 gaactcattt tccagcgtgt tagaaacacc ggttttctgc gctacctgct ggaacaggct
    22921 gcccatgttg acgattgcaa ttttgtcagc cgcctgagca gaagttgcca gtgctaaacc
    22981 gagacctgca gctaataacc actttttcac aataaactcc ttaccatccc atttgcaccg
    23041 gagggtgcag ttctttgcgt ggcccggcga tcttatattg atcgcctaaa gtcatcgcta
    23101 cactaccact acattccttt gtggagaaca cttaccaggt tttaccgatg ttaaactgga
    23161 actgttctgc cttgtctcca tcgtactttt tgaacggctg ggcgtaggag aacaccaacg
    23221 gccccaatgg ggacatccat tgtaatgcga tacccgcaga catacggata ttgcttggat
    23281 cactatagtc cggatatcca gaatattggc tggaatccca gtttgtatcc caaacggtac
    23341 ccatatccca gaagaaggaa gtacgaaccg agttagcata cttatcgcta ataaacggcg
    23401 tcggggtgat gaactcgagg ctggcaaccg ccatggcgtt accgcctaca gcatcatccg
    23461 atttacacag gtctttcgcg ccgtcctgag tcgcacattc gtaatcatag tccggatcat
    23521 aattactggc ctgatgcggg aagtaaactg ctttcggacc aattgtattg gactggaagc
    23581 cacgcacggt gctggaacca ccggcataga agttctcgta gaacggcatc tctttgccgc
    23641 ctaaaccatc accataaccc cagcgggtac gccccagaac aacccatttg tgatcgtcat
    23701 cgatcggcac ataagtcgcc gtgtctaacg tcactttgta gtattcgtta tccgatccag
    23761 gaatggtcac tttaccggtc aggttgacac gtgaaccatc tgtcgggaag taaccacggt
    23821 caagcttgtt ataggtccaa ccatagttga acgtgaagtc gtccgttttg aagctattat
    23881 cctgatcaga ggtgctcgga tgttcaccca tagagtacag ataacgccac atcgcaacct
    23941 gaggctgcat gttggacagg gagttatgta cataacccag acctgcacgc agcgagttat
    24001 attcgttgat cgggaagccc aacgtcacgt ctgtaccata actcttgttg gtatagtcgg
    24061 acaggtcggc gtcatctgcc tggaagtcat tatagaagag acgaccaccg aggcttacac
    24121 catctacagt gaagtacggg ttggttaccg acagttcagc ataggtctgg taatcgtttt
    24181 tggtcccgtt gataccaaca gcataacctg tacctaacca gttatcctgc tgcacaccag
    24241 cctggaagct cacgccactt tcagtaccgt aaccaatacc aaagttgaag ctaccggtgt
    24301 tgcgctcttt taccttgtag acgacatcta cctggtccgg gctacccgga acacgttggg
    24361 tatcggtatc gacagtttca aagaagccca gacgattcag acgctcctta ccctgatcga
    24421 ccagatcgct tcccagccat gcaccttcca tctgacgcat ttcgcgacgc aggacggcat
    24481 ctttcgaggt atcgttacct tcaaaacgga tcttacgcac gtagaaacgg ttacccgcat
    24541 caacgttcac gcgtaattta acggttttgt cggcatcgtt aatttcaggc atcgactgta
    24601 cgcgcggata ggcataacca tagcgaccga gaagcttttt gatgtcatct tccatcttgg
    24661 tcactttggt gccgttatac agctcgcccg gctcgatctt agtcagctgc tcaatttcag
    24721 cggagtgccc ggcaaggttg ccgctcactt caacgccaga aagcttgtac tgatcgcctt
    24781 cggtaatgtt caccgtgacg taaatacctt ttttatctgg cgtcagactg acctgggtag
    24841 agtcgatgtt gaaacgggca taaccgcgat ccagatagta gctgcgcagg gtttcaaggt
    24901 cgcccgccag tttctgtttc tggtatttac gatcgcctac cacgttccac cacggcactt
    24961 cgtcacgcag ttggaaatga gagatcagtt cgtcggtggt gaaagcatgg ttaccaacaa
    25021 tgttaatttg ctggatttca gctgacacac cttcctggaa caccagtttt aggtcaacac
    25081 ggttgcgcgg cagcggggtc acgacagctt ttacgctggc gctatattta ccgacgctgt
    25141 agtagaagtc ttccagacct ttctcgatat cggcaatggt ggtgcgatcg agggattcgc
    25201 ccacacgcac accagaagcc tcgaggtttt gcttcagcat gtcatctttc accgatttgt
    25261 taccggagaa agtaatgctg gcaatggtcg gacgttcttt tacctgaacc agaagggtat
    25321 caccatcacg aaggacgcga acatcctcaa agttgccggt agcaaacaga gcgcgaatgg
    25381 tattactgat atcttcatca ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg
    25441 ccgcaccaac ggcgacacgc tgaaggcctt cgaaatgaat atctttcact acgaaccctt
    25501 cagcaccgta tacggtggcg ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg
    25561 ccatcgttat tatgcgttct tcctaactaa ctctcataac cgagagaaat cattgaaaag
    25621 tgcaagcccc attaacagca ccagcagaat cgagccaatg cgataacaaa agtcttgaac
    25681 ccgctcggat accggtccgc ccttgatctt ttcgatcgca aggaacagca gatgcccccc
    25741 gtcaagtacg ggcaacggaa acaggttaat tatccctaag ttcacgctaa taagcgcaag
    25801 aaacggcagg taataaacta ccccgagttc cgctgtcatc ccagccccct tggcgataga
    25861 gatcggccca ctgaggttgt tcagtttcac atcaccggtg atcaattttc ccagcatact
    25921 gaccgtcagc ttcatcagct gccacgtttt gtccgtggct tcgacgatgg cgttgaacgg
    25981 cccatactgg cgtacaactt tatactcatc tggcaaagga atgactttcg gctcaatacc
    26041 gacaaaacca atcgctttac cattacccgg tttactctcc gggattaatg tcaaagacaa
    26101 gggacttccc tgcctttcga tttctaacgc taaggattta cccgggttat cccggacaag
    26161 catcacaaag gtcacccact gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc
    26221 tgcttgcaaa cctgccttgc ttgccgccga gtttggctgc acattttcca gtacaggttc
    26281 aatttgcggc ccacgaggac gaatccccag cgaagatacc ggatcttctt tatcaggctc
    26341 aaacgcccag tgacgtaaat cgagctttac atcccgccgt tgatcgctgc caaatggcgc
    26401 tactgtaatg gtggtgcttt catcgccaat tttatcgacc aactgcaaac gcacggcatc
    26461 ccaatcaggc gtttcgatac catctacggc ttttagttcc gtacctggtg caatttgtgc
    26521 ttccgcagct atcgaattgg ctgctatttc accaaccacc ggacgtacgc caggcacacc
    26581 aataataaaa accagccagt aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc
    26641 ggcaataatc gccgctcgtt ggccaacaga tttattattg aaggcatggt ggcggagttc
    26701 cggaacgacc ggttctgcgc gctcatccag cattttgaca taaccgccca gcgggatcag
    26761 ggcgataaca tattcggtgc cgagcttatc agttcgccgc cagagcgcct taccaaaccc
    26821 tattgagaaa cgctcaacgc gaacaccaca acgccgggca acccagaaat gaccaaattc
    26881 atgcacggtg ataagtacac ccagtgcaac gatgaacgaa gccaaatccc agagaaaact
    26941 cagcatatta ccttccgtta aagcgtcctg aataccagta acaacaagca agcaaagacc
    27001 ggtaccgcag ccgtcaggct atcaatacga tctaaaatac caccgtgtcc tggaattaaa
    27061 tgaccgctgt ccttaattcc tgcttcacgc ttaaacatac tctcggtcag atcgccgagc
    27121 actgaggcta acgctgcgac aatagagcaa atgagtaagg tgacgggagc gacgtcgaga
    27181 ttcgcccaca tgccataacc ccatgagatt accgctgcgg tcgctagtcc acctataaag
    27241 ccttgccagg ttttacccgg agaaaccttc ggtgccagct tatgtttacc aaacaatttg
    27301 ccaaacatat atgcgccaga gtcagcgccc cataccagga tcatgacata gagcagccat
    27361 attgcgccac tgtaatgatt ctcgtcgtag tgccaggccc gtaacgccag catgccccag
    27421 aagaagggaa caatggttag cacgccaaaa ataaggcgca atgttttaga gttacgccag
    27481 attgctgcgg aacctgggta aaacagcacc agcaatagcg cgacaatcca ccaacccagc
    27541 gaagcccaaa gtgagatttc aaccagcggt tgatgaatat ttcggtgata ttccggcaac
    27601 agaaaaagca tcagcgccaa caataacccg cataacaccg ccaaccatac tcgctgcgaa
    27661 cgagtggtaa aaccgctaag ctgtccccat tcccacgctg ccagcatgca gaccaccagc
    27721 gttacgatgg cgaaccccac cggcggcaac agaaacaacg ccgcgatgac gacgggtatt
    27781 aacacaaaag cagatatcag gcgatacttc agcaaaagcg acccccatca ggctgtttca
    27841 tcaccgggct cggtgccgcc gaaacgacgc tctcgattag caaaggcatt taacgcccct
    27901 tcaaagtctt gttcatcgaa atcgggccag agaacatctg taaagtaaag ttcggcatag
    27961 gcaatttgcc aaagcaaaaa gttactaatg cgatgctccc ccccagtcct aattactaaa
    28021 tctacagggg ccagttcatg catacagaca tgctggttta gcatctcttc atctatctga
    28081 tctggttgca ggtttccttg ctgcaccttt tcagccagtt gcctgactcc ctggactata
    28141 tcccaacgtc caccgtagtt cgccgcaata ttcagcgtca gaccggtatt cccggctgtt
    28201 agcgcttcag atttacgaat acgttcttgc aaacgcgagt taaagcgact ggtatcgcca
    28261 ataatacgca gacgcacgtt atgtcggtgc agacttttta cttcgctatc gagcgcccac
    28321 acaaacagtt ccattaacgc actgacttcc tgcgctggtc ggttccagtt ttcactacta
    28381 aaggcataca gcgttaacgc ctcaataccg ttgttggccg caaaagagac agcccggcgg
    28441 acggattttg ccccggcttt atgcccaaag gcacgaatct tcccttgctt ttttgcccag
    28501 cgaccattgc cgtccataat gatcgcaaca tgacggcagc catgcgctgg caatttttcg
    28561 ctaagtggtt gagtagcaga caacatcacg cgttttttat tccctgacag ggtttacggt
    28621 actcaggaat actgaagcct atacaaaaaa aagccgtgta aaaccacggc atacctgacc
    28681 ttataaagcc aactacttac gatcagatgg cgcagactat atcactgaag ccctacgcta
    28741 acaaatagcg cgactctctg tagccggatt atcctcagct tgcgagacgc atcacctctt
    28801 ttctggcgac ttcacgcgcg ttcgcatcaa cagataacac atcgtccaca cattgtggtt
    28861 cgcgcatatc cattttttcc agtacggata aattcaacgc agcgatatcc gtaaagcgga
    28921 tttgttgcgc aagaaaagca gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg
    28981 ctgcctgacc ttgttcgaac gcctccatcg ccagtttcag gcatggataa cgatcataat
    29041 ccggtgcggc aaatgtcaac gcacttagtt tgcaaaaatc gagcggcttc acgccagagt
    29101 tcacgcgatt cggccatgcc atggtgtggg caattggcgt acgcatatcc ggttccccca
    29161 gctgcgccag aacactgccg tcctgatagc gcaccattga gtgaatcact gactgcgggt
    29221 gaatcagcac ttccatctgg ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt
    29281 ccagaccttt gttcatcatg gtagccgaat cgacagaaat tttacgcccc atcgaccagt
    29341 tcggatgacg gcaggcttga tccggcgtca ttgttgccaa atcgcgcaat ggcgtctcac
    29401 ggaaagggcc accagacccg gtaagtaaaa tggacaccac gccattttgc tcaaggtcag
    29461 cgtatcccag attatgctgg ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc
    29521 tatcgaccgg taacaattgc gctttgctct gctttacggc gtccataaac agacgtccgc
    29581 aggtaaccag tgattcttta ttggccagca aaatggtttt acccgcgcgg atcgcagcaa
    29641 gcgtaggtaa cagcccagca gcgccaacaa tggctgccat cacctgatca acatcctcaa
    29701 gcgctgccat atcgcaagcg gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt
    29761 gctgtagcat cgttttaaga agtttcgcac tcgcttcatc gtccattacg gcatagcggg
    29821 gagagaattc caggcactgt tctaccatgc gagtgacatt tttgcctgcc accagcgcaa
    29881 ctacgcggaa gtgttcggga ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc
    29941 cggtcgagcc cagaatggtg agttgcttca tgaaacatcc agagttgaga cagaataaaa
    30001 agcaaaacgc cgccagccga tccgcaagga tctactgagc ggcgtttttg tcgttcaaga
    30061 aatcagaact gcatcagttc tgcttctttg tctgccagcg ccgcttcaat tttcttgatt
    30121 gcagcatcag tcagtttctg tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc
    30181 tctttatctt tcaacagtgc tttcactttg tcgttcgcgt cacgacgcac gttacgtact
    30241 gcaacacgcg cttgttctgc ttcaccacga acgattttgg tcagatcttt acgacgttct
    30301 tccgtcagcg gcggcagcgg aacacggatg tcgctacccg cagagttcgg gttcaggcca
    30361 agatcggacg ccataatcgc tttttcaacg gccggagaca ttgaacgatc aaacacgttg
    30421 attttcagag tacgggaatc ttctaccgtt acgcttgcca gctgacgcag cggcgtcggc
    30481 gtgccgtaat attccacgac aatgccatcc agcaggctgg gagaagcacg acccgtgcgt
    30541 attttgctga tttgggtttt gaacgcttct acgcatttgt ccatgcgtac ttcagcatct
    30601 tttctgatat cgctaatcac gttacgaatc cttgaaaact tgtctcagtc agaccagaca
    30661 gtcacacaca gtggaagtgt gctaagtata gccctgatta aacatattac gggtaatcgt
    30721 ctggattatt aggctatttt atttgccatt ttggccccgg gtagtgctcg ccatcctcac
    30781 gtacttgtgt acgcaccggt tgctgcgcgc tggccgtgac caaactgcct gcaacaataa
    30841 cgccttataa ccagacttac gcccccgcga tacttatgcg gaatcttacc cttatttatc
    30901 catcacggga attattccgt gattaaagtc ccttcttttt cacccattac cacacggcgc
    30961 agcgcacccg gtttgttcat gttgaaaaca cgaatcggta atttatggtc acgagccagc
    31021 gtgaaggccg ccaggtccat gactttcagc tctttttcca gcacttcgct gtaagtcagt
    31081 tgctcgtaca tggttgcggt tggatctttc gccggatcag cggtaaacac gccgtcaact
    31141 ttggttgctt tcagcaccac atcggcttca atttcgatac cacgcaggca agctgctgag
    31201 tcggtggtaa agaacgggtt acctgtaccg gcggagagga tcaccacacg gttgttgcgc
    31261 aacaggctga tagcttctgc ccagctgtag ctgtcgcaca cgccattcaa tggaatagcg
    31321 gacatcagac gagcgttcac ataggcgcgg tgcagtgcat cacgcattgc caggccgttc
    31381 attacggtcg ccagcatccc catgtggtcg cccacaacgc ggttcatacc cgctttcgcc
    31441 agaccagcgc cacggaacag gttaccccca ccaatcacca caccaacctg aatacccagt
    31501 tcaaccagtt ctttgatttc ctgagccata cgatccagta tgcttgcatc aataccgaag
    31561 ccttcagtgc cctgcagagc ttcgccactc aacttaagca gaatgcgttt atagacgggt
    31621 tttgcattgg tagccatgtt tctttcctga gactgtcaac gattgaaatg aattaatact
    31681 agcgacattg tacgtcgctt ttcagcgcag ccccacaagg gttagctgaa tttacaagat
    31741 gggcacaaaa agaagccgcc ctcaggcggc tccttttcga taattaagac tgcttggaca
    31801 tcgcagcaac ttctgctgca aagtcagtct caactttctc gatgccttca cccacttcga
    31861 agcggatgaa gccagtcact tcagcgttat gctctttcag cagctgacca acagttttgc
    31921 ttggttccat aacgaacggc tgaccggtca gagaaacttc gccggtgaat ttcttcatgc
    31981 ggccttcaac cattttctct gcgatttctt tcggcttacc agactgcatc gcgatatcca
    32041 gctgtacctg gtattctttt tctaccactt cagcggatac gtcttctggt ttgatgaatt
    32101 ctggcttgct tgcagcaacg tgcatagcga tgtgtttaac cagctcttcg tcagcgcctt
    32161 tagcagcaac cagaacgccg atacgcgcac cgtgctgata agaacccaga acgtcgcctt
    32221 ccagcgcagc aacgcggcga atgttgatgt tttcaccaat tttcgctacc agcgcaacac
    32281 gttcttcttc gaactgtgct ttcagaactt caacgtcagt gattttgcca gcaacagctg
    32341 cgtccagaac tttgtctgcg aacgcctgga aaccagcgtc ttttgcaacg aagtcagtct
    32401 ggcagttaac ttccagaatg atgccgtagt tgccgtcgat tttggttttg atcacgccgt
    32461 cagcagcaac gttgcctgct tttttcgctg ctttaatagc accggactta cgcatgtttt
    32521 cgattgccag ctcgatgtcg ccgttagctt cagtcagtgc ttttttgcaa tccatcatgc
    32581 ctgcgccagt acgctcacgc agctctttta ccagggatgc ggtaatttca gccattctaa
    32641 aatcctcgga gatgtgatct gcccggcatt aaaccgcaca gatataaaag tgaaaaaggg
    32701 ggccatatgc aggcccccta accaaacgtg tactacctgg tctataaggg ctctccccct
    32761 tcatatttcg cgccgcagat gcgttatctt cgctcgctca tcccggtcac ttactgatgt
    32821 aagctcccgg gaattctcga gcttgccgcc tttctgcaac tcgaactatt ttgggggagt
    32881 tatcaagcct tattactcag cttctacgaa gctttcttcc gcctgggaag ccagatcctg
    32941 agaacggcct tcacgtacgg ttgcagcaac agcgcccagg tacagggtca cagcacggat
    33001 tgcgtcgtcg ttacccggga taacgaagtc aacaccgtcc ggatcagagt tggtatcaac
    33061 gatagcaaat accggaatac ccaggttgtt tgcttctttg atagcaatgt gttcgtggtc
    33121 agcatcgatt acaaacagag cgtccggcag accgcccatg tctttgatac cgcccaggct
    33181 gttttccagt ttctccagct cacgagtgcg catcagcgct tctttcttgg tcagcttttc
    33241 gaaagtaccg tcctgagact gagtttccag gtctttcaga cgtttgatgg actgacgaac
    33301 ggttttccag ttagtcagca taccgcccag ccagcgatgg ttcacgaaga actggtcgca
    33361 gctcagagca gcgtctttca ccgcttcgct tgcagcgcgt ttagtaccaa cgaaaaggat
    33421 tttacctttg cgagaagcaa tcttgttcag ttcagccaga gcttcgttga acatcggtac
    33481 agttttctca aggttgatga tgtgaacttt gttacgcgca ccgaagatga acggcttcat
    33541 tttcgggttc cagtaacggg tctggtgacc gaagtgaaca ccagccttga gcatgtcgcg
    33601 catggaaaca gttgccatga ttaaaacctc tatataaaag ttggggttat gcctccacgt
    33661 atcccatatt accgacccca aagggcaccc cggaatatgt gccgatacgt gtgtgttgtt
    33721 acacaaagtg agattgtcgc ttccgtccag tctgtgtata cgaaatggat cggaagtccg
    33781 gcgcgcttta taccacaaat acgtcatgga caccaataat tgttggcgct gtgtacagca
    33841 tcagatgtcg aattttctat tatagaaaac cttcagtggc acgtttggcg aaattcagaa
    33901 tgattctcaa tttgcccggg tgtgatacca ttgacggcac ttacatatat attgtcggta
    33961 tcaccgacgc tgatggacag aattaatggc tatctcaatc aagaccccag aagatatcga
    34021 aaaaatgcgc gtcgctggcc gactggctgc cgaagtgctg gagatgatcg aaccgtatgt
    34081 taaaccgggc gtcagcaccg gcgagctgga tcgcatctgt aatgattaca ttgttaatga
    34141 acaacacgcg gtttctgcct gcctcggcta tcacggctat ccgaaatccg tttgcatctc
    34201 tattaatgaa gtggtgtgcc acggtatccc ggacgatgct aagctgctga aagatggcga
    34261 tatcgttaac attgatgtca ccgtaatcaa agatggtttc cacggcgata cctcgaaaat
    34321 gtttatcgtc ggtaagccga ccatcatggg cgaacgtctg tgccgcatca cgcaagaaag
    34381 cctgtacctg gcgctgcgca tggtcaaacc aggcattaac ctgcgcgaaa tcggtgcggc
    34441 gattcagaag tttgtcgaag cagaaggctt ctccgtcgtt cgtgaatatt gtggacacgg
    34501 tattggtcgc ggcttccatg aagaaccgca ggtgctgcac tatgactccc gtgaaaccaa
    34561 cgtcgtgctg aaaccgggga tgacgttcac cattgagcca atggtcaacg cgggtaaaaa
    34621 agagatccgc accatgaaag atggctggac ggtaaaaacc aaagatcgca gcttgtctgc
    34681 acaatatgag catactattg tggtgactga taacggctgc gaaattttga cgctacgcaa
    34741 ggatgacacc atcccggcga taatctcgca cgacgaataa gatgaagccg gcgaatgccg
    34801 gcttttttaa tgcgataatt taatcttatg ggtggcgcac aatgaatacc cttccagaac
    34861 agtacgcaaa caccgctctc cccaccctgc ccggtcaacc gcaaaatcca tgcgtctggc
    34921 cccgtgatga attaaccgtc ggtgggataa aagcccatat cgatactttc cagcgttggc
    34981 tgggtgatgc ctttgacaat gggatctctg cagaacagtt gattgaggcg cgcaccgagt
    35041 ttatcgacca gctcctgcaa cgattatgga ttgaagcggg attcagccag attgccgacc
    35101 tggcattggt cgccgtcggt ggctacggtc gtggcgagct gcatccactt tctgacatcg
    35161 atttactgat tttaagccgt aaaaagctcc cggacgatca ggcgcaaaaa gtgggcgagc
    35221 tgttaacgct gctctgggat gtaaagctgg aagtcggtca tagtgtgcgc acgcttgaag
    35281 agtgcatgct ggaagggtta tcggatttaa ccgtcgccac caatttaatc gaatcccgct
    35341 tattaattgg cgatgttgcg ctcttcctcg aactgcaaaa acatattttt agcgaaggat
    35401 tctggccttc cgacaagttc tacgcggcga aagttgaaga acagaaccag cgccatcagc
    35461 gttaccacgg caccagctac aatcttgaac cagacatcaa aagtagccct ggcggcttgc
    35521 gcgatatcca cactctgcaa tgggtggccc gccgtcattt tggcgcaaca tcgctggatg
    35581 aaatggttgg gtttggcttc ttaacctcag cggagcgggc ggaattaaac gaatgtctgc
    35641 atatactgtg gcgtattcgc tttgccctgc atctggtcgt cagccgttac gataatcgtc
    35701 tgttattcga tcgccaactt agcgtcgctc agcgtctgaa ttacagtggt gaaggcaacg
    35761 aaccggtcga gcggatgatg aaggattact tccgcgttac acgccgcgtc agtgaactca
    35821 accagatgct gctgcaactg ttcgatgaag ccatcctcgc ccttcccgcc gacgaaaaac
    35881 cacgtccaat cgacgatgag tttcagctac gcggtacgct aatcgacctg cgtgatgaaa
    35941 cactatttat gcgccagccg gaagccatct tgcgtatgtt ctacaccatg gtgcgcaaca
    36001 gtgcgatcac cggcatttac tccaccacgc tgcgccagtt acgccatgcc cgtcgccatc
    36061 tgcaacaacc gctgtgtaat attccggaag cacgaaaact gtttttgagc attctgcgtc
    36121 accccggagc ggtgcggcgc gggctattgc caatgcatcg ccatagcgtg ctcggcgcgt
    36181 atatgccgca atggtcgcat atcgtcgggc agatgcagtt tgatctgttc cacgcctaca
    36241 cggtggatga acatactatc cgcgtgatgc tgaaactgga gagttttgcc agtgaagaaa
    36301 cgcgccagcg ccatccgttg tgtgtggacg tctggccgcg cctgccgtca actgagctga
    36361 ttttcatcgc cgcgctgttt cacgatatcg ccaaaggacg cggcggcgac cactccattc
    36421 tcggtgctca ggatgtagtg cattttgcag aactccacgg gctgaactca cgcgaaacac
    36481 agctggttgc ctggctggtt cgccagcact tgctgatgtc ggtgacagca caacgccgcg
    36541 atattcagga ccctgaagtc atcaagcagt ttgccgaaga agttcaaacg gaaaatcgtc
    36601 tgcgctatct ggtatgcctg acagtggctg acatttgcgc caccaacgaa acgctgtgga
    36661 atagctggaa gcaaagtctg ttgcgcgaac tctattttgc caccgaaaag cagctacgac
    36721 gcggaatgca aaacacgccg gatatgcgcg aacgggttcg ccatcaccaa ctccaggcac
    36781 tggcactact gcgcatggat aacatcgacg aagaggcgct gcaccaaatt tggtcacgct
    36841 gtcgtgctaa ctattttgtc cgccatagcc caaatcaact ggcctggcat gcccgccatt
    36901 tattacagca tgatttaagc aaaccgctgg tattgcttag cccgcaggct acgcgtggag
    36961 gcaccgagat ttttatctgg agcccggacc gcccctatct gtttgccgcc gtctgtgccg
    37021 aattagaccg ccgcaattta agtgttcacg acgcacaaat tttcaccact cgcgacggta
    37081 tggcgatgga tacctttatc gtgctggaac ccgatggcag cccgctgtcc gcagatcgtc
    37141 atgaggttat tcggtttggt ctggagcaag tactgacgca aagtagctgg cagccaccgc
    37201 agccccgtcg ccaacccgcc aaattacgcc attttactgt tgaaaccgaa gtaacgtttt
    37261 tgccgaccca taccgaccgc aaatcgttcc ttgagcttat cgcgctcgac cagccaggac
    37321 tgctggcgag agtcgggaaa atttttgccg atctgggaat ttcgcttcat ggtgcccgaa
    37381 ttacaaccat tggcgagcga gtagaagatt tattcataat tgccaccgct gaccggcgtg
    37441 cacttaataa cgagttgcag caggaagtgc atcagcggtt gacagaggcc ctcaatccaa
    37501 acgataaagg gtgatgtgtt tactgatatg aaaagagttt aacaatgcag cagttacaga
    37561 acattattga aaccgctttt gaacgccgtg ccgagatcac gccagccaac gcagacaccg
    37621 ttacccgcga agcggtaaat caggtgatcg ccctgctgga ttccggtgca ctgcgtgtag
    37681 cggaaaaaat tgacggtcag tgggtgacgc atcagtggtt gaaaaaagcg gtgctgctct
    37741 ctttccgtat taatgataat caggtgatcg aaggggcaga aagccgctac ttcgacaaag
    37801 tgccgatgaa attcgccgac tacgacgaag cacgtttcca gaaagaaggc ttccgcgttg
    37861 tgccaccagc ggcggtacgt cagggtgcat ttattgcccg taacaccgtg ctgatgccgt
    37921 cttacgtcaa catcggcgca tatgttgatg aaggcacgat ggttgatacc tgggcgaccg
    37981 tcggttcttg tgcgcagatt ggtaaaaacg tccacctttc cggtggcgtg ggcatcggcg
    38041 gcgtgctgga gccgctgcag gctaacccga ccattattga agataactgc ttcattggcg
    38101 cacgctccga agtggttgaa ggggtgattg tcgaagaagg ttccgtcatc tctatgggcg
    38161 tatacattgg tcagagcacc cgtatttacg accgtgaaac cggcgaaatc cactacggtc
    38221 gcgttccggc ggggtctgtg gttgtttcag gtaatctgcc gtcgaaagat ggcaaataca
    38281 gcctctactg tgcggttatc gttaagaaag ttgacgcgaa aactcgcggc aaagtcggca
    38341 ttaacgaact gctgcgtacc atcgattaag agtacatata cgggcagcat gatgctgccc
    38401 gtatgctttc tggctcagaa taattcgtcc tactcattct tcttcccccc ttttttaaat
    38461 actttttcat cttgtttcgg actgtcatgt aattcgcaaa acgaagataa atgtgttaat
    38521 ccatcccatg gaatatcacc acaataaagc aggtaatttt ttgattttta cgccatgata
    38581 gaagtctgga agaaaaaggc tttctgcgat cacagtcgag ccgggcgggc caggccaaat
    38641 gctaaaataa gcacaattta ctgactcatt ttgaggtaac gctatgtacg acaatctgaa
    38701 aagtctgggt attaccaatc ctgaagaaat tgatcgttac agcctccggc aggaagccaa
    38761 caacgatatt ctgaaaatct atttccagaa agacaaaggc gagtttttcg ccaagagcgt
    38821 taagtttaaa tatccgcgtc agcgtaaaac ggtcgtcgct gatggtgtgg gtcagggtta
    38881 taaagaagtc caggaaatca gcccgaatct acggtatatc attgatgagc ttgatcaaat
    38941 ctgccagcgt gaccgcagcg aagttgatct taagcgtaag atcctcgacg acttacgtca
    39001 cctggagtca gtcgtaacca ataagatcag cgagattgaa gccgatctgg aaaaactaac
    39061 gcgtaaataa tgccgaactg acggttaact cggacattcc cctctaccct tcgggctgag
    39121 gggaatgagc cagcgccgcc tcgaccttaa cgcataacct accgctcttc atccagctgc
    39181 aacgccacat acagcagcaa cctgtcatca aaattaccca aatcaagccc ggtcagttcc
    39241 gatatacgat taagccgata ctccagagta ttacgatgaa taaacagcgc ctttgacgta
    39301 gccagcggtt gcacattgtg gcgaaaccac gccgccagcg ttcgtcgcag caagccatta
    39361 ttgtccatcg ctttcagccg cgccagcggt cgcgccagtt cgttggcctg ccagtcgcca
    39421 cgcaaactgt cgagtaacac aggtaacatc agatcctgat aaaaatagca gcgactttct
    39481 ggcatccgct gtttacccac caccatcgtc gttttcgccg tacgatagga tcgggcaata
    39541 ctgccaggac cggtaaaata gttgcccagc gaaacgcgaa aacgcagctg gccatactct
    39601 ttcatgcggg taatcagttg ttcaactcgc ttacgatgat cttctgcatc ccagcgccca
    39661 aaagagttca acgccggttt caacaccacc atttcggtca gcgagacaat cgccaccaga
    39721 ttattacgct cgggcgtagt cagcgcattt tgcagttgtt gtaactccgc cattgcgctg
    39781 tccacgccaa gctgaccgct gtcgacctca acaatagcca ctactcgcgg ttgattgaga
    39841 tcgatcccca gccgttgcgc ccattcagta agtgcgggag tattctcctc agcctgaatc
    39901 aggttcatca ccagttcttc ccgcaaacgg ctatcctgcg ccaacaggtg catcaaccgc
    39961 gactgttcca gcatcatttc agccgtcatg cagaccagtt cgccatattt acgcagattc
    40021 tctggttcac ctgtcaggcc aattacgccg acaatttcac cttccagccg taacggtaga
    40081 ttaatccctt gccgcacacc gtgcagatga cgtgccaccg cgtcatcgat atcgacgact
    40141 cgtccctgtg aaagtaccag caatgcacct tcgtgcaatt caccaatacg ctcacgatcg
    40201 ccgctgccga taattcgccc acgggcatcc attacgttga tattggtatc gatgatgcgc
    40261 atggtacgtg ccacgatatc ctgcgccatt ttggtatcaa gatgccagcc agccataagt
    40321 cctccgttat gcacggctta gcataaggaa gtacgtaacg tacggcattg tgcaaatgcc
    40381 taaaggatga tgaagatgta tggagttgtg gtgaagttca cagattgtaa ggagaacccc
    40441 ttcccgtttt caggaagggg ttgagggaga ttactgcatt aacaggtaga tggtgctgtc
    40501 gccgcgctga atgttcagtg ccagcacaga cggtttgctg tcgagaactt tacgcagttc
    40561 agcgatgttt ttcactgcct gctggttcgc gccaataatc acatcacctt tcttcaggcc
    40621 gatctgcgca gccggagtgc ccgttttcac gttgttcact accacgccct gatctttgcc
    40681 tttgttgctc atctcagcgc cttcaatgcc gttgaagatg gagctggaat caacctgatt
    40741 ctggctgctc tgctgcagtt ccagattcac gttgacctgc ttaccgtcgc gcagcaagcc
    40801 cagggtcagt ttgctgccca ccggcatagt acccacctga gcacgcagtg cggcaaagct
    40861 gctgattggc ttaccgttca gtgaggtgat cacatcaccc gctttaatgc ccgcttttgc
    40921 agcggaggaa ttaggcagaa cctggcttac gaaagcaccg cgctgggcgt caactttcat
    40981 cgctttcgcc agttcggaat tcagctcagt ccccataata cccagctcac cgcgtttcac
    41041 ctggccgtat tccaccatct gcgaggtcag gtttttcacc atgttgctcg ggatagcaaa
    41101 accgataccg atgttgccgc cgtccggtgc gaggatcgcg gtgttgatac cgatcagttc
    41161 gccgttcagg ttaaccagcg caccaccgga gttaccacgg ttgatcgctg catcggtctg
    41221 gatgaagttt tcgtagtttt cggcattcag gccgctacgc cccagcgcag agacaatccc
    41281 ggaagttacc gtctctccca ggccaaacgg gttaccaatc gctacggtgt aatcacccac
    41341 gcgcagtgca tcagaatccg ccatcttaat tgcggtcagg tttttcgggt tctggatctg
    41401 aatcagcgcg atatcagagc gcggatcttt gccaaccatc ttcgcgtcga acttacggcc
    41461 atcgctcagt tgaactttaa tgaccgtcgc gttatcaaca acgtggttgt tggtgacgac
    41521 atagccttta tcggcatcaa tgatgacgcc ggaacccagc gccatgaatt tctgttgctg
    41581 gccgccacca ttaccgccct ggccaccctg gcagaacgga gagctctgga acggagaacc
    41641 ttcctggcag aacggagaat catcaccgaa gaactgctgg aaattacgcg gcatacgcgg
    41701 cgtattaacg gttgtgctac cttctacgtt aatgctgacc actgaaggca tcaccttttc
    41761 gagcatcggt gcaaggcttg gcatctgctg ggctgtcgtt gctgaagaag tctcagccgc
    41821 cgttgcagag agcggagata acgccaaacc taaactcaga gccagtgcac tcagtgctaa
    41881 tgtggttttt ttcatgtatt tcagtctcga ttaacagata acgcaaaatt gctgtgttct
    41941 tcagattcgt tttatagcgc gaagttccga actaaagttt ctgcaaaagg taaaaattta
    42001 ttgttcgtct ttacaaaagc ctggttattg ttctacggcc atcagacgtc ggtattcatc
    42061 ccacgcatag aggtcggtca taccgctgat ataatcctgc agcaggcggc aacggtaata
    42121 atattcccat agcggaaact caggagaatc tgacggtaat ttactgacag cctcgacata
    42181 ggccagccga tggcgcgtcg agagtttgtg gaataagcgc gattcaatag ggaaacgttt
    42241 cacccgttct ttttctacca gttcagtaaa gtctgataac gacaggctta ataaaggacg
    42301 ataaatctct aataatccgc taatgacccg atagccctgc aattcaagct gctcgacatc
    42361 tggatggcta aacacatgtt ttacagcgac atttttatat agcttaagaa gatcgctgca
    42421 ttcgctggca tcttccaata atgcatgatt aaacgttccg gcgaaaatcg caggcagatt
    42481 atcaataaat cgttgtgccg catagggtac cagtttattt agggtattta ctcgtaaata
    42541 cataaaaaac tgatcttccg tactgcggct taaactattt gagcgtgatt tttcccaggc
    42601 attttcaacc accagcgaaa agagcgaacc tttctcatgc tggccccacg cttcgtgcaa
    42661 atgatgataa agctgctcaa cggtaaatat tcttttctct accgcatctt caaggtctgc
    42721 cacacaatag gagatgtcgt cggcagcttc cataatccac gttaatggaa aacgactgta
    42781 aagcgccaaa ttaagttctt tacgcaaccg ggcaatatag gcttcttcag aaagataata
    42841 acccggcttt ttcattaaat agtgatgtgt ctcaggcgtt tcgccacgcc accacgccgg
    42901 acgggtatat tttaaaatac cgccaacctg tgcccaggtg agattcatcc gcatcaatgt
    42961 atgcaccaga cgaatgcctt gtgcattccc ctcaaaatga cataagtcct gacgaatctt
    43021 gcgccgcagc tcgttaagcg gttcttcccc gtcccgtaaa cgtagtgccg ccacgctgca
    43081 gcgatcgtca gtcagaggct ggctttcggc atcttccggg tgcaaacgtt ggcgaaacca
    43141 gtcatttatc gctgcttcgc caaaatgacc aaacggcgga ttgccgatat cgtgcatcag
    43201 gcatgacatc tcaacaatgc tttcaaaagg tccggtcagt tcatccaggc cgtatgcttc
    43261 cagcaattta agctctttca gacggcttaa aatttctttg gcgatgtagc gccccacctg
    43321 ctggacttcc atcgagtgag taagacgcgt gcgcacggcg gcattgcgct ccagtggaaa
    43381 aacctgggtc ttttgttgca gacgacgaat tgccggagag ttgatgatac gcccgcgatc
    43441 gctctcgaag atccgcagga tctcatgttc ggttttaacg ccctgcggtg aacggtaacg
    43501 acgatgccag tttatttttt ttcggaaatc aatctgtgcc atgtcctctc ccgcgtgaga
    43561 aatacgcttc cccgtaagcg catggtaaac tatgccttca aatcgggctt atcgcgagta
    43621 aatctatgaa aatcggcatc attggtgcaa tggaagaaga agttacgctg ctgcgtgaca
    43681 aaatcgaaaa ccgtcaaact atcagtctcg gcggttgcga aatctatacc ggccaactga
    43741 atggaaccga ggttgcgctt ctgaaatcgg gcatcggtaa agtcgctgcg gcgctgggtg
    43801 ccactctgct gttggaacac tgcaagccag atgtgattat taacaccggt tctgccggtg
    43861 gcctggcacc aacgctgaaa gtgggcgata tcgttgtctc ggacgaagca cgttatcacg
    43921 acgcggatgt cacggcattt ggttatgaat acggtcagtt accaggctgt ccggcaggct
    43981 ttaaagctga cgataaactg atcgctgccg ctgaggcctg cattgccgaa ctgaatctta
    44041 acgctgtacg tggcctgatt gttagcggcg acgctttcat caacggttct gttggtctgg
    44101 cgaaaatccg ccacaacttc ccacaggcca ttgctgtaga gatggaagcg acggcaatcg
    44161 cccatgtctg ccacaatttc aacgtcccgt ttgttgtcgt acgcgccatc tccgacgtgg
    44221 ccgatcaaca gtctcatctt agcttcgatg agttcctggc tgttgccgct aaacagtcca
    44281 gcctgatggt tgagtcactg gtgcagaaac ttgcacatgg ctaagtcact gttcagggcg
    44341 ctggtcgccc tgtcttttct tgcgccactg tggctcaacg ccgcgccgcg cgtcatcacg
    44401 ctttctcccg ccaacactga acttgccttt gccgccggga tcacgccggt tggggtcagc
    44461 agctattccg actatcctcc acaagcgcaa aagattgagc aggtttccac ctggcagggg
    44521 atgaatctgg aacgcattgt cgcgctgaaa cccgatctgg tgattgcctg gcgtggaggt
    44581 aatgccgagc ggcaggttga ccagctggct tcgctgggaa taaaagtgat gtgggtcgat
    44641 gcgacaagca ttgaacaaat tgccaatgcg ttacgtcaac tggccccctg gagtccgcaa
    44701 ccagacaagg ccgaacaagc cgcgcaatcc ctgctggatc agtacgcgca attgaaagcg
    44761 caatatgctg ataaacctaa aaaacgtgtt tttctgcaat tcggcattaa tccgccattt
    44821 accagtggaa aagagtcgat tcagaaccag gtactcgaag tttgtggcgg agaaaacatc
    44881 tttaaagaca gccgggttcc ctggccgcaa gttagccgcg aacaggtgtt agcacgctcg
    44941 ccacaggcga ttgtcattac aggcggaccg gaccaaattc ctaaaatcaa acaatactgg
    45001 ggtgaacagc tcaaaattcc cgttattcct ctcacgagtg actggtttga acgtgcaagc
    45061 ccacgtatta tcctcgctgc acaacagctc tgtaatgcgc tttcacaggt agattagtgc
    45121 ctgacctttt caggtgctca gtggggattg aacaatgctc gtctattggc tggatatagt
    45181 cggcacagcg gtatttgcca tctccggcgt tttgttagcc ggaaaattgc gtatggaccc
    45241 ttttggtgtt ctggtactgg gcgtggttac cgctgtaggc ggcgggacaa ttcgcgacat
    45301 ggcgctggat cacggcccgg tattttgggt gaaagatccc accgatctgg tcgttgcaat
    45361 ggtcaccagc atgctgacca tcgtgctggt gcgccagcca agacgcttac caaaatggat
    45421 gttgccggtg ctggacgccg ttggtctggc ggtgtttgtc ggcattggcg tgaataaagc
    45481 ctttaatgcg gaagccggtc cgttaatcgc ggtttgtatg ggcgtcatta ctggcgttgg
    45541 cggcgggatc attcgtgatg tactggcccg cgaaatcccc atgattttac gtacagaaat
    45601 ctacgcaact gcctgtatta tcggcggtat tgtccacgct acggcttatt acacattttc
    45661 cgtaccactg gaaacagcca gtatgatggg catggtcgtg acgctattga ttcggctggc
    45721 ggctattcgt tggcatctta agctaccgac gtttgcgctg gatgagaatg ggcgttgaag
    45781 agagaagatg tgccggatag tttatccggc acatgaacaa cagattagat actaaaggaa
    45841 gaaccgcaac cgcaggtgct tttcgcgttc gggttggtca cgatgaaacg agaaccttcc
    45901 agaccttcgg tataatcaac ggaaccgccg accagatatt gcaggctcat cggatcaacc
    45961 accaggccaa cgccctgttt ttcgatggtc atatcgcctt cgttcacctg atcatcaaag
    46021 gtgaaaccat actggaagcc gctgcaaccg ccaccggtga tatacacgcg taatttcaga
    46081 ttcgggttat cttcgtcagc gatcaggctt ttaactttgt tggctgctgc gtcggtaaac
    46141 tccagcggca gtgctacgtc atcactcata ttttgctcca aacgacatcg gcaattgggc
    46201 aaattctaac ccaataataa tcgccattat ctaataccct ggtatttcgt tcaagtattc
    46261 tcgctggctg atgcggcctt gcttcgcgcc agttgctcag cttcctgttt tgccagcgtg
    46321 cgcgcaagaa tcgccgagta tagcggtttc ccgccggtaa attgcgctaa tagtgttgcg
    46381 ccaagaccgg taataatcat tggcaaaatg agctggtagt tatcggtcat ctccagaacc
    46441 agaatgatcc ccgttaacgg cgcgcgaata gatgccgcca gtaatgcccc cattccggca
    46501 atagcaaacg tccccgcctc caggtgatat tgcggaaaca gctcaacggc aaccattccg
    46561 aaagcggttc ccagcacagt acccagcgcc agcatcgggg caaaaatacc gcccggcgcg
    46621 ccggaagaga agcagagtaa ggtggtaatg acccgagcga cgaagataaa caccagcatt
    46681 cccatgctga aattccccgc ggtagcgata ggaatcaggt taaaaccgcc gcccgacgtt
    46741 gctggtgcca caaaccccag caatccacac agaccgccaa tcgcaccacc catcagtacc
    46801 catttggtaa tattgccgcc gtgcacacgg tgcagcaaat cctgcatccc cagcacccat
    46861 ttattaaaaa tagggccgaa aatgccaaaa ataataccga ggatcagata aagccacagc
    46921 gtattaagcg gcgcatcaga aagtttaccg acgtcaatca acgcaacttc atgattaaaa
    46981 atccggtaca taatggtcga cataatgacg ccaataaata ccgctttaat cgaaattaac
    47041 gtatagcgaa actgcggacg catctcttcg ataataaaca aaatacccgc cagcggcgcg
    47101 ttaaaggccg cagccagccc cgcagcagca ccggttgcca gcagcgtatg gcgagcttca
    47161 tcacctttca ggcggaaaat atcaagcacc atgcgaccga tattgccgcc gatctgcacg
    47221 gttggtcctt cgcgccctag caccatgcct ccgccgagtg tccccagccc gccaaagaat
    47281 ttcaccggca acacacgcca ccagcgaacg ggacgttgat cttccagcgc cccttcaatt
    47341 tccgggatcc ccgaaccacc tgcttccggc gcgtatttgc gcaccaaaaa gtagccgaac
    47401 atcgccagca ccgccgaaca gagaaaagcg acggttaaca gaagcggata attatcagca
    47461 gtatgtacca gcgcccccat acgttggttc tgcaaccagg cgacaccttt gtcaaaagca
    47521 accgctgcca gcccgacaag cgtgccgacg actgccgcca taaacaaaat ggctaacggg
    47581 gttttatcgc gctcaagaag ttggcgaatc agttgtctgc gtcgcaggcg cgcggcctgc
    47641 ggtgtttcta aagagggagt atcagttttc atccaatgat cacttattgg tcatacaaat
    47701 aagatgacgg cattttactc gcacgttcca tgaaaatcct ttgcaaagcg taatgtttca
    47761 aatgcacaaa acttttatac tctaagaaat caggattgca ccagtacaag cagcctgatg
    47821 tttgacgagt atttaacttg ttatgaataa catagaatag cagccattca ctttttctac
    47881 gaatcaggaa ccctccatga gtaagtctga aaatctttac agcgcagcgc gcgagctgat
    47941 ccctggcggt gtgaactccc ctgttcgcgc ctttactggc gtgggcggca ctccactgtt
    48001 tatcgaaaaa gcggacggcg cttatctgta cgatgttgat ggcaaagcct atatcgatta
    48061 tgtcggttcc tgggggccga tggtgctggg ccataaccat ccggctatcc gcaatgccgt
    48121 gattgaagcc gccgagcgtg gtttaagctt tggtgcaccg accgaaatgg aagtgaaaat
    48181 ggcgcaactg gtgaccgaac tggtcccgac catggatatg gtgcgcatgg tgaactccgg
    48241 cactgaagcg acgatgagcg ccatccgcct ggcccgtggt tttaccggtc gcgacaaaat
    48301 cattaaattt gaaggttgtt accacggtca cgctgactgc ctgctggtga aagccggttc
    48361 tggcgcactc acgttaggcc agccaaactc gccgggtgtt ccggcagatt tcgccaaaca
    48421 taccttaacc tgtacttata acgatctggc ttctgtacgc gccgcgtttg agcaataccc
    48481 gcaagagatt gcctgtatta tcgtcgagcc ggtggcaggc aatatgaact gcgttccacc
    48541 gctgccagag ttcctgccag gtctgcgcgc gctgtgcgac gaatttggcg cattgctgat
    48601 catcgatgaa gtaatgactg gcttccgcgt agcgctagct ggcgcacagg attattacgg
    48661 tgtggaaccg gatctcacct gcctgggcaa aatcatcggc ggtggaatgc cggtaggcgc
    48721 attcggtggt cgtcgtgatg taatggatgc gctggccccg acgggtccgg tctatcaggc
    48781 gggtacgctt tccggtaacc caattgcgat ggcagcgggt ttcgcctgtc tgaatgaagt
    48841 cgcgcaaccg ggcgttcacg aaacgttgga tgagctgaca tcacgtctgg cagaaggtct
    48901 gctggaagcg gcagaagaag ccggaattcc gctggtcgtt aaccacgttg gcggcatgtt
    48961 cggtattttc tttaccgacg ccgagtccgt gacgtgctat caggatgtga tggcctgtga
    49021 cgtggaacgc tttaagcgtt tcttccatat gatgctggat gaaggtgttt acctggcacc
    49081 gtcagcgttt gaagcgggct ttatgtccgt ggcgcacagc atggaagata tcaataacac
    49141 catcgatgct gcacgtcggg tgtttgcgaa gttgtgatga ttttgcctcg cccaccgggc
    49201 gaggcattca attaacggct ctgctttctc aacaaataga taaaatatgg cgcgccgata
    49261 aaggttgaca gcagccccgc cgggatctgg aatggaaaca gcaccatccg cccacaccag
    49321 tcagcgaaca ccagcagtaa accacccacc agcgccgaaa ttacgatgtg tggcatcgtc
    49381 cgtcgaaagc ccatcatccg cgcaatatgc ggtgccatta aaccaacaaa actcaacggt
    49441 ccaatagtca tcgtcgcggt cgccgtcagg caagccgcta acagcagcag cgcaattcgc
    49501 gtcggcgtca gcgccattcc tacggctcgg gcggtatcac cacccagcgg taaaatggtc
    49561 agccagcggc ggcacagcgg ggtaatcgcc agcaaaatca ccatcacaat tccggtgcgc
    49621 cagacctgcg catcggtcgc gttgtaggtc gaaccggaaa tccaggtcag cacttgcgcc
    49681 attcgcgggt caccacttgc ctgcaacatc atcaaaagca tggtgaacgc ggtacttaac
    49741 gccatccccg ccagtaacat gcggtgtggg gaaaatccac cgcgtccggc ggcgatcata
    49801 atgatcaaca gcgtcaccgc cgccccgaga ctgcctgcgg gtaacagcca gccaaaggca
    49861 ttacccggta ccagaaacag cattaacacc acaccaaacg ccgcgccgga gctaatcccc
    49921 agcacttccg ggcttgccat cgggttgccg gtcagccgct gaataataca gcccgctacc
    49981 gccagcatga cgcccgcaaa cagcgccgcc ataattcgcg gccagcgcca gggcattaaa
    50041 tcatcgagca gcgccccgct cgcccacgtc cagccgtgcg catcacgacc aaacgacagc
    50101 gccaccacca cagccatcaa cagcagcacg ccgcccgcga gggcaaacgc cagcacatgt
    50161 tggcgttcag tcgcgacacg atcgttaacc ttcatatccg gcgcgctaat gctgcgtaaa
    50221 cgcggcagca gccacagtag cagcggcgca ccgatcaacg cagtgaccga accggtggaa
    50281 acttccatcc acacgcgagt cagccagagg atgatttgat cagaaagcca cagaattaac
    50341 gcaccaatca gcgacgccag catcaaccgt ggcaacaaac gccgcgcccc cagcattttt
    50401 gccagcagtg gcgcgaacaa cccgataaag ccgataatcc ccactgcgtt caccagcagc
    50461 gcactgatga ctatcgccag cgacagcgcc gccagacgcg cgagcgacaa ggccagcccg
    50521 agattgcgcg ccacgccatc atcaagcccc atcagggtta acggacgaag tagcagtaac
    50581 gtcagcatca cgccgcccag caactgcggc cataaacgct caacgccgcc ccagtcggtt
    50641 tgcgtcagcg ttccggtgct ccacaggaac atgctttgca actggtcatg atggaagata
    50701 accagtaact gattgattgc cccacaataa aggctcacta ccagccccgc gagaatcagc
    50761 gttaccggcg acagccgttt cccccacgcg acgccaaaga caattaagcc aacaacgcaa
    50821 gcccctgcca gcgcagcaaa ctggctcgcc atagcacctg ggatcgccca gagcgtggtg
    50881 acggtaatcc ccagttgcgc gcctgtagca acgcccaacg tcgtcggttc cgccagcggg
    50941 ttacgcagca cttgctgaaa cagcacgccc accagcccca ggcccgcgcc caccagtaga
    51001 gaaatcgcca gacgtggtaa caagctgtag tgaaaaatca tctgctcgat gacgttaata
    51061 tccggcgacc aggcggcctg cgcccactgg ctacgcggca gcgcctgcga aaagttcatc
    51121 caggtcagcg ccgtagcgac aatcaccagc agcgccagca ataacgccgg aaaaagcgca
    51181 attcgtttac tcacgcttta cctccgatgg cgttatccag aacgcgcaca aagtgcattg
    51241 ccgagagcgt cgcaccataa aaccagactg cgggtacgcg ctgaaagcgt ccggcgcgga
    51301 caaacggcat ggcctgccac agcggggttg ccattagcgc gtccatgtct ttgctgttgt
    51361 cgtgatcaaa acagagcaca tcaacatctt tatacgccgc cagacgatcg atgctgacgg
    51421 cggtactgcc ccagaagttg gtttcccctt gccaggcatt tgggatgccg tactcatcaa
    51481 gaatttcctg aaacaagctg tttggaccga aaaccagcat atggcgcgga tcgataagcg
    51541 tcgtcagcaa taacgggcgt tcaccacgct tcacaaagcg gggtttcatg ctgcggataa
    51601 agtcttcata gtgcgctaaa tgcgtttctg ctgcgctttg caggttaagt aaatctgcca
    51661 tttccgtcag tgacttacgc gccatcgcca gcggctgttt gccgtcgctg aagttaaatc
    51721 cacgccccgg cgcaatacga gccagcattt ctggtgaagg gccatatcct gccgaccaga
    51781 ccataaacga tggtttcatt tcggtcagca gttcaaggtt aggttctgtg cgcaaaccga
    51841 cgtcgatcac tgagtccggc aatggtggtt cgctgaccca caggcgatag ttgatggtat
    51901 ccgccacgcc gtaaggcacg atgccgagcg ccagcagtaa ttccaccggc aaccactcca
    51961 gcgccacaat acgattggga tcaatagccg ccgcgtgggc ggtattcatc tgccataaca
    52021 acggagaaag cgccatcgcc gttaacagtc gacggcgcga aataagaggt aagccgctca
    52081 tcaataaaca aaactcacag gtgcagcacc cgccggatgc ggcaaaatac ccatcgggat
    52141 gccataaatc atttcgaggg tttcgccgcg cataatttcc gcaggcgttc cctgagcaat
    52201 catttcaccg ccgcgcaggg cgaccagata atcacagtag cgtgccgcca tattgatatc
    52261 gtgcaacacg gcaatgaccg tcaggccacg ctcctgactt aaacggtgca ccagcgacag
    52321 cacatcaacc tggtgggcga tatccagcgc cgaggtcggt tcgtcgagca acagacaacg
    52381 gctatcctgc gccaccagca tggcgatcca cgcccgctga cgttcgccgc cagagagact
    52441 atcgaccagc cgatgcgcca gcggttttaa gccaaccagc gagatagctt cctcgacctt
    52501 ttcgcgatct gccgcaccaa agcgccccag cgcgccatgc cacgggtaac gaccaatcgc
    52561 caccagttca cgcacggtca tcccttctgc cggaggaagc tgctgcggca aataagccac
    52621 tttgcgggca aacgctttgc tgctccagct ttccagcggc tgggcatcaa gaagaatctc
    52681 cccttctgac ggaggctgat gacggccaag cattttgagc agagtggatt taccagaacc
    52741 gttgtgacca atcagaccgg tcactttccc ggcaggaaag gttaacgaca gcggatgcaa
    52801 aagcgtgcgc ccgggcacac gaaaggagat attacgcagt gcaaaagtgg tatcggaatg
    52861 attcgtgtat tcctgcataa cagccaactt gtgaaatggg cacggaaatc cgtgccccaa
    52921 aagagaaatt agaaacggaa ggttgcggtt gcaacgacct gacgttctgc gccccagaag
    52981 cagccataag tgttaaagca gctggcgacg tattcacgat cgaacaggtt gttaacatgc
    53041 agcgccacgt tggagcccgc catgccgact cgcgccagat cataacgcac taacgcatcc
    53101 acgaccgtat aacttcccac tttaaaggag ttagccggat caccatagct ggagccagta
    53161 taacgaccac cggtgcccag cgtcagacct gaaagcggac cgtcaaagaa ggtatagtca
    53221 gcccacagcg aagccatgtg ttttggcacc tgtgcaggcg tattgccttt ataggtagta
    53281 tcggtggtgt attccgcatc ggtgtaagta taagaaccga ctacgttaac actcgccgac
    53341 agcgccgctt tcgcttcgat ttctacgcca cgtgcgcgga tctcgccacc ttcaaccgag
    53401 aagaaggaac cctcagggtc cgccatcagg ttgttggttt tagtgagatt atacacggca
    53461 ccagtaacta caatcggacg atcttccggt acatatttca cgccgacttc atactgctta
    53521 cctttagacg gtgcgaaaat attaccatcc ttcccaactt gcgaagaagg ttcaaacgat
    53581 tcgctatagc tgaagtaagg tgttacacca ttatcaaaca ggtagttaac accaccacgc
    53641 caggtaaact gtttgtcatc acgtttatcg gtcgtcccag caacgcggtt aagagattct
    53701 tgatctgccc agtcataacg accgcccagg gtgaccagca ctttatccca ctgcgcctga
    53761 tcctgaacat aaacgcccgt ttgtttctgc ttattcagaa tgcggtaagg gccggagttt
    53821 gccggatctt tggcattgaa gtcgaaatcg gtattcaccg gattgtacag attgagcagt
    53881 ggcacagagt cgtcgtaacc aaaccaggcg ttgatgtcat tacgcatacg cataaagtcg
    53941 acaccggtca gcagggtgtg gtcgatatcg ccagtggcaa acttgctctg caactgggta
    54001 tcaacggaga agttttgcag cttctcatca tcaacgacgt atttacgtgc cagataatgg
    54061 cctttatccg ctggcgctaa tgccgcacac tgtttgctgt aagcattcgc cggatcggag
    54121 cagacgccgt aaccataaac gctgttttgc gaggttttgt tttcagcaaa gcgcaggttc
    54181 tgacgcacag taaaggtgtc gttaaattcg tgatcgaagc tgtagccgac catcttctca
    54241 ttacgagaat aggtgttgtt cttcgcccct tcattaaagt ctgtcggcag acgcttaccg
    54301 ttcggcagcg gctcaacggt tccctctttc ggcaaccagc cgtaataacc ggtttccggc
    54361 tcgttctgga agtaagaaag gaaggtgaaa ttggttttat catccggacg ccaggtgaac
    54421 gccggtgcaa tagcataacg ctgctcttct gaccctttct gctgggcatt ggcagaacgc
    54481 gcaagaccgg tcaggcgata agagtaaaca ccgtcatcat ccaacgaatc gctaaagtca
    54541 aaaccagtct ggaacaggct gtcagtaccg gctttaaact gaacttcttt cagcggttcg
    54601 gtggtcggac gcttgctgac catattcaac aggccgccag gactgctttt accgtaaagc
    54661 acggaaaccg ggccacgcat aatttcagcg cgttccagca tatacgggtc aatgaccgca
    54721 tcgttataga agttgccctg caacttcagg ccattcagat agttattctg gctttggcct
    54781 tctgccgcaa agccgcgaat gatcaggtgg tcataggtgt tggatgcgcc acgcgtacca
    54841 acagagacac ccggcgtgta gctaagcgct tcttttaccg acttcggctg atgcagcgcc
    54901 atctcttcgg cggtcacaac agaaatagac tgtggcactt tttgaatcgg cgtatcggtt
    54961 ttagtgccgg tagcagactg tcgcgccgca atagttgcag caggccccca tgcgctttct
    55021 tgcggcgcag gtgcagcggt aacggtgata gtgtcttctt tcggttcaac cgctgcctgt
    55081 gcataaacag acatgccgct taccgctgtg gctactacaa ctgcgatttt acgcagtgag
    55141 tgttttggct gagcagtttt ggaacgcgcc attggtatat ctctgatgta aagtgaatga
    55201 taacgtaaac gagaatgatt attataaaga taagataata ttcgaaacgc tggcgggttc
    55261 gcaagcagta cgcaatctga gcgttcctga agggttaata aacaaccaga tgaaaagaaa
    55321 gggttaatat cttagatggg atgttatttt accggatggc aattcgccat ccggtatttc
    55381 acgcttagat gttaattact accaaacata tccttgatcc aaccggctac accgtcgctg
    55441 tctttctgct cttgctgagc aggttgctgt tgcggctgtt gctgcggctg agaagactga
    55501 tcaaacggat tgcctgacgg ttgctgctgc atctcactct gctggcacag cgattgcgga
    55561 tcgctggtcc agaccggcaa gacacgcatg ccaccgctgc aaacaaagtt gccgtcgtag
    55621 tccacgccca tatctgcaat atcttctggc ggaacaagat tcagcggcgt tggcgtctgg
    55681 ttagccagat aacgctgata aatcgacatt gccccgctgg caccatacag tttggtcggc
    55741 tggttgttat cacggcctac ccaggtgatg gtcaccgtgc tgccgtcaat gcccgcaaac
    55801 caggtatcta cgttattgtt ggtagtccct gttttccctg ccagatgcag gttcgggtat
    55861 ttcgccccaa gttgacgacc cgtaccacgt tgtaccacct gctgcatggt ccatagtgtc
    55921 agatacgccg cctgcgccgg aacagcgcgt tctgcctgcg ggaagctctg atacagcacc
    55981 ttgccatctt ccgcgattac cgaacgcagt gcagaaagcg gtgcacggtt accaccgctg
    56041 gcgatggtct ggaatgcctg cgccacttcg attggcgtta agttcaacgc ccccagcagc
    56101 attgccggaa ccggatgcaa ctgatctttc ggcacgccca gtttgatcca ggtctccgta
    56161 accgcaggca gccccagcgc catccccaga tttaccgtcg gcacgttcat cgaacgggtc
    56221 aacgcatcca ccagcatcac tctgccactt tcgctataac gacgatcatc gttctgcggt
    56281 gaccagacct ggccattcgg ctgacgcagc gcgattggcg catccgcaat ccacgtattc
    56341 agacgataga ttttcggctg gcttaaagcc gtcaggtaag tcgctggttt tgcaagggaa
    56401 ccaatcgaac gacgcgcctg catcgcacgg ttgtagcccg caaactgcgg ctcagaacct
    56461 ccgaccatcg cacgaacttc accactaaag cggtcgacga ccacaatcgc agtttcaaga
    56521 tcgctcaact tacgctgttt cttcagtgcc ggaatgcctt ccacggcggc tttttctgcc
    56581 gcgtcctggg ccaccgagtc aaaggtagtg aagatcttca cgccggagag atcttttacc
    56641 ttatcgccca gttttgcctg cagctcctga cgcaccagtt gcataaaggc tggctgagga
    56701 gagatcaccc caccgcgcgg ctgaaccccc agcggacggg cactcaacat gtcatagagt
    56761 tcttgatcaa taatctgttg ctgttgcagc agacgcagca ccagattacg tcgctccagc
    56821 gccagttttg ggttacgcca cgggttgtag atggacgccc ctttcaccat accgactaac
    56881 agcgcctgct ggtccaggct tagctcttct accgggcgac caaagtaata caagcttgcc
    56941 agcgggaagc cgcggatttc gttgtcgccg ctctgaccga gatacacctc gttcatatac
    57001 agctcaagaa tacggtcttt gctgtaacgc gcgtccatga tcagcgccat gtaagcttcg
    57061 ttcgctttac gccagtaaga acgctcgctg gagaggaaca ggtttttcac cagctgttgc
    57121 gtcagcgtac tcgcaccctg taccgtgcgt ccggcggtca ggtttgccag caccgcacgt
    57181 ccgattgagt agagactgat tccatcatgc tcgtaaaaat gacggtcttc tgtcgccagc
    57241 aaagtatcca ccagcaaatc cgggaaacca ctgcgcggca caaacagacg ctgctcaccg
    57301 tttggcgaag agatcatggt gatcagacgc ggatcaagac ggaagaaacc gaactgacgg
    57361 ttgttctcca tattgacgat cgtcgccaga tgatcgccat caaaggtcag acgcgcgcgc
    57421 acctgtcctt ctttactgtc cgggaaatca aacggacggc gaatcatctc aatgctgttg
    57481 gcctgcacgg taaattcgcc aggacgggtc attttcgaca cctgacgata ctgggtcgcc
    57541 tccagcagct tcaccatctc gttcttgctg atggtcatgt ctggctcaag attgaccatt
    57601 cggccataaa ctgccgcagg cagttgccag accttgccat caatacggct acgaattttt
    57661 tgatcgagat aaacgccgta aatggcgatc agcacggcaa aaacgatagc cagttttagc
    57721 agtagccata gccagccgcg tttgccacga ggcttacgcc ctttgccttt gcccttacct
    57781 ttgcgcggca tcggttcttc atcctcatag tcatcataat cgtcgtaatc atcgtcatct
    57841 tcgtaacgac gacggcttac cttttgtttg accggacgcg tcggtttccc tttgcgtcca
    57901 attggctcgc ggtcattccc ggccatgctt tttctccgca atattcaggc gcaaaagccc
    57961 gattttctgt tcttctgtta caagacagaa gaagatatct ctcaaaattt gtgctcgatc
    58021 ggtcccctct cccttgaggg agagggttag ggtgagggtg aacgtgcgcg ctgatgccct
    58081 caccccggcc ctctcccaca gggagaggga gaaaaccaac cttacgaata ctttttcgtc
    58141 cgtcgcgtcg gtgcagtatt tgccgggtcg tccggccaga catgtttggg ataacgccct
    58201 ttcatctctt tttgcacctc acgatacgcc cctttccaga aggcccccaa atcacgcgtg
    58261 atttgcagcg gcctttgggc aggtgaaagc aactccagca ccagcggcac gcgcccctgg
    58321 gcgatcgtcg gattggtggc ctcgccaaac atctcttgca ttctcaccgc cagcgcgggc
    58381 gggttatctt catgataacg aatggcgatc cggcttcccg tcggcacagt gtaatgcgca
    58441 ggcaattcac tatccagacg ttgctgcatt ccccaatcaa gtaatccgcg tagtgcctga
    58501 taaatgtcga gtgatttcag gccgcgtagt gaatgtacgc cagtcatatg tggcagcagc
    58561 cacgtttcca gcgctgccaa taaactttca tcatcaaccg ctggccagtc atattccggc
    58621 aaccactttg cggcgcataa caaacgcaag cgtagctgtt ccgcttccgc cgtccagttg
    58681 agcacgctta aacctttatc acggatgcca ttaagcatcg cctgatgcaa ctcgtcttct
    58741 gacggtttcg ccagcggctg cactttcacc gtcaactgac cgatttgcag ccgacgccag
    58801 gctttcagcg taccttgcgc gtcatcccac tccacagtgt cagactgctg taccagctgc
    58861 gggcagcgtt gtactaactc atcaatatcg accggcagcg ccagtaaaat ccgcgcatcc
    58921 ggcgaggcgc tgccctgcaa taataacggt gcgatcaacc attcgtggcg gcttagcgcg
    58981 tcgttggcat cgagcatcgc tcccatgccg tttgccagtt gatagcgtcc atcttgccca
    59041 cggcgacgag caatgcgatc ggcaaacgcc ccggcaagta gcggcgcgat aagcgaactg
    59101 tctgcctcac cgccacgtac gtttaagcgt tttaacagtt gctgactacg ttgctgccag
    59161 gctggttgat tgcgcgaaaa cgccacgccc aggtcactat tgcccatccg tggcggctct
    59221 tcgagaatgg cggcaatttt tgccgcggta gcagcttcgt cgtcgttctt cgcgctaacc
    59281 agcattgccg ctaaacgcgg atcgttaccc agcgctgcca ttttttgccc ttgcgcacta
    59341 agccgttcac cctccagtgc ccccagcatt tgtaacagac gtttcgcggc cagtagattc
    59401 actactggcg gttgatccag ccagctcatc tgcgccggat cgctgcatcc ccattgcagt
    59461 aattccatca gcaaaccgga aagatcgctt tgtaatatct ccggttcact ttgcgccgcg
    59521 gcgcgttctg cttgttcctt ggcgattaaa tgcaggcaga tacccggctc cagacgcccg
    59581 gcgcgcccgg cacgctgcgt catggatgcc tggctaacgc gttgagtaat cagtcgcgta
    59641 agccccgtgc gcggatcaaa acgcgccaca cgctcctggg cacaatccac caccagacga
    59701 ataccttcga tggttaaact ggtttcagca atattggtcg ccagcaccac tttgcgcatc
    59761 ccttgcggtg ccgggaggat cgcttttcgc tgatcgttca gcgacaacgc gccatacagc
    59821 gggcagagca atacatcgct gccgatgcgc gaagccagtt gttcctgcac acgctgaatt
    59881 tctccgacgc caggtaaaaa taacagtaat gatccacttt cctgacgcag catttcagcg
    59941 gtggctaccg caacggcttc gtcaaaacgc tgatgcgtgg gcagtggtaa ataacggcgt
    60001 tcaaccggaa acgagcgccc ttctgagatg acgacgggcg cttctggcag catttgctgc
    60061 aagcggtcgt tgtccagcgt agccgacata atcagcagtt taaggccatc acgcagacct
    60121 tgttgcacat cgagtaacag cgccaacgcc aaatccgcct gcaagctgcg ctcatgaaac
    60181 tcatcgagga tcaccaatcc aacaccgctc aactccgggt cacgctggat catccgcgtc
    60241 agcacgcctt cggtaactac ttccaggcgg gtattcggcc cgacgcagtt ttgcgcacgc
    60301 atccggtagc caacggtatc gcctggcttt tcgttaagca gctccgccag ccgttgcgcg
    60361 acgttacgcg ccgccagacg acgcggctcc agcaggataa ttttcccgtt aatgccggga
    60421 tgcgccagca gttgcagcgg cagccaggtt gatttcccgg ccccggtcgg cgcacttaat
    60481 aatacctgcg gcgcacaatc gagagcggta agtaattcag gtaagacggc agcaacgggc
    60541 aacgacgaca caaaacgctc cagagggtta acattcttcg cgccacattg tagcatcgcg
    60601 gtaattcata accgagtgcc tcacatgtct gaaccgcaac gtctgttctt tgctatcgac
    60661 ttacctgcag aaatccgcga acagattatc cactggcgcg ccacacactt cccacctgag
    60721 gcgggacgtc cggtcgccgc cgataatttg catctgactc tggcattttt aggcgaagtg
    60781 agcgccgaga aagagaaggc gctttctctt ttagccggac ggattcgtca acctggtttc
    60841 acactcacgc ttgatgacgc cggacaatgg ctgcgttcgc gtgtggtgtg gttagggatg
    60901 cgtcagccgc cacgcggctt aatccagctg gcgaatatgc tccgttcaca ggctgcccgc
    60961 agcggttgtt ttcaaagcaa tcgtccgttt catccacata ttaccttatt gcgcgacgcc
    61021 agcgaggcgg tgacaatccc gccgccaggt tttaactggt cgtatgcggt gacggagttc
    61081 accctttacg cctcctcgtt tgcccgtgga cgcacacgct acacgccgct aaaacgctgg
    61141 gcgctaacgc aataacaagg attgtcgcaa tggaattttc tccccctcta cagcgcgcga
    61201 cgctaattca gcgttacaaa cgttttttag ccgatgtgat cacacccgat ggtcgcgaat
    61261 taacgctaca ctgcccgaat acgggtgcaa tgaccggttg tgcaacgcct ggcgataccg
    61321 tctggtattc gacttcagac aacaccaaac ggaaataccc acacacctgg gaattaactc
    61381 aaagccagag cggcgcattt atttgcgtca acacgctttg ggctaacagg ttgacgaaag
    61441 aggctatcct taatgaatca atttcagaac tgtcaggcta tagctcgctg aaaagcgaag
    61501 taaaatacgg cgcagaacgc agccgtattg actttatgtt gcaggcggat tcgcgtccag
    61561 actgctatat tgaagtgaaa tcggttacgt tagcggagaa cgaacaggga tattttcccg
    61621 atgcggtcac tgaacgaggt cagaaacacc ttcgggagtt gatgagcgta gcggctgaag
    61681 gccagcgtgc ggttatcttt ttcgccgtgc tgcattcagc cattacacgg ttttcacccg
    61741 cgcgccacat cgatgagaaa tacgcgcaac tattgtcaga agctcaacag aggggggtag
    61801 aaattctggc ttacaaagcg gaaatttctg ctgaaggcat ggctcttaaa aaatcactgc
    61861 cggttacatt gtagtaaagt aagtaactgg ttaatttaca ttctggtcgc gtgcgcaaat
    61921 acgcttttcc tcacacagtt gtcaagtgtt acgtttagat aattgctatc cggaaaagca
    61981 tctgctattt atagcggcct catttttccc ccgaacatgg ggatcgatag tgcgtgttaa
    62041 ggagaagcaa catgcaagaa gggcaaaacc gtaaaacatc gtccctgagt attctcgcca
    62101 tcgctggggt ggaaccatat caggagaagc cgggcgaaga gtatatgaat gaagcccagc
    62161 tggcgcactt ccgtcgtatt ctggaagcat ggcgtaatca actcagggat gaagtcgatc
    62221 gcaccgttac acatatgcag gatgaagcag ccaacttccc ggacccggta gaccgtgcag
    62281 cccaggaaga agagttcagc ctcgaactgc gtaaccgcga tcgcgagcgt aagctgatca
    62341 aaaagatcga gaagacgctg aaaaaagtgg aagacgaaga tttcggctac tgcgaatcct
    62401 gcggtgttga aattggtatt cgccgtctgg aagcgcgccc gacagccgat ctgtgcatcg
    62461 actgcaaaac gctggctgaa attcgcgaaa aacagatggc tggctaatta cagccgttcc
    62521 atcacgttta ccacaggcgg gaaatgctcc cgccttattt tttgttcaaa gagatgacag
    62581 acacacagta tattggccgc ttcgccccct ctccttccgg cgagcttcat tttggctctc
    62641 tgatcgccgc gctcggcagc tatttgcagg ctcgcgcccg gcaaggtcgc tggctggtac
    62701 gcatagaaga tatcgacccg cctcgtgaag ttcccggtgc cgcagaaact atcctgcgcc
    62761 agctggaaca ttacggtctg cactgggacg gcgatgttct ctggcaatcg caacgtcacc
    62821 acgcctatcg tgaagcactc gcctggttac atgaacaagg actaagttat tactgcacct
    62881 gtacgcgtgc gcgtattcaa agcattggcg gtatttacga cggtcattgc cgggtgttgc
    62941 atcatggacc agacaatgcc gcagtgcgta tccgccagca gcatccggtc acgcaattta
    63001 ctgaccagct gcgcggcatt attcacgccg acgaaaaact ggcacgggaa gattttatca
    63061 ttcatcgccg tgatgggttg ttcgcctaca acctggctgt tgtggttgat gatcatttcc
    63121 agggcgttac agaaatagtg cgtggggctg atctgattga accaacagta aggcaaatct
    63181 cgctgtacca gctttttggc tggaaagtgc cagattacat tcatctgccg ctggcgctta
    63241 atccacaagg cgctaaactt tccaagcaga atcatgcgcc tgcgttgccg aaaggcgatc
    63301 cacgcccggt actaatcgcg gcacttcaat ttctggggca gcaggcagaa gcacactggc
    63361 aggatttcag cgtcgagcaa atccttcagt cagccgtcaa aaactggcgg cttaccgccg
    63421 tgcccgagtc ggcaattgta aattcaacat tctcaaatgc gtcatgctga gctatgatta
    63481 gccgctattt ttttgtcctg aatgatgttt gacactaccg aggtgtacta tttttacccg
    63541 agtcgctaat ttttgccgca aggtgctaag ccgcgaggaa agcgaggctg aacaggcagt
    63601 cgcccgtcca caggtgacgg tgatcccgcg tgagcagcat gctatttccc gcaaagatat
    63661 cagtgaaaat gccctgaagg taatgtacag gctcaacaaa gcgggatacg aagcctggct
    63721 ggttggcggc ggcgtgcgcg acctgttact tggcaaaaag ccgaaagatt ttgacgtaac
    63781 cactaacgcc acgcctgagc aggtgcgcaa actgttccgt aactgccgcc tggtgggtcg
    63841 ccgtttccgt ctggctcatg taatgtttgg cccggagatt atcgaagttg caaccttccg
    63901 tggacaccac gaaggtaacg tcagcgaccg cacgacctcc caacgcgggc aaaacggcat
    63961 gttgctgcgc gacaacattt tcggctccat cgaagaagac gcccagcgcc gcgatttcac
    64021 tatcaacagc ctctattaca gcgtagcgga ttttaccgtc cgtgattacg ttggcggtat
    64081 gaaggatctg aaggacggcg ttatccgtct gattggtaac ccggaaacgc gctaccgtga
    64141 agatccggta cgtatgctgc gcgcggtacg ttttgccgcc aaattgggta tgcgcatcag
    64201 cccggaaacc gcagaaccga tccctcgcct cgctaccctg ttgaacgata tcccaccggc
    64261 acgcctgttt gaagaatcgc ttaaactgct acaagcgggc tacggttacg atacctataa
    64321 gctgttgtgt gaatatcatc tgttccagcc gctgttcccg accattaccc gctacttcac
    64381 ggaaaatggc gacagcccga tggagcggat cattgaacag gtgctgaaga ataccgatac
    64441 gcgtatccat aacgatatgc gcgtgaaccc ggcgttcctg tttgccgcca tgttctggta
    64501 cccactgctg gagacggcac agaagattgc ccaggaaagc ggcctgacct atcacgacgc
    64561 tttcgcgctg gcgatgaacg acgtgctgga cgaagcctgc cgttcactgg caatcccgaa
    64621 acgtctgacg acgttaaccc gcgatatctg gcagttgcag ttgcgtatgt cccgtcgtca
    64681 gggtaaacgc gcatggaaac tgctggagca tcctaagttc cgtgcggctt atgacctgtt
    64741 ggccttgcga gctgaagttg aacgtaacgc tgaactgcag cgtctggtga aatggtgggg
    64801 tgagttccag gtttccgcgc caccagatca aaaagggatg cttaacgagt tggatgaaga
    64861 gccgtcaccg cgtcgccgta ctcgtcgtcc acgcaaacgc gcaccgcgtc gtgagggtac
    64921 cgcatgacag tggcgtatat tgccataggc agcaatctgg cctctccgct ggagcaggtc
    64981 aatgctgccc tgaaagcatt aggcgatatc cctgaaagcc acattcttgc cgtttcttcg
    65041 ttttaccgca ccccaccgct ggggccgcaa gatcaacccg attacctcaa cgccgccgtg
    65101 gcgcttgaaa cttcgctcgc cccggaagag ttactcaacc atacacagcg catagaactg
    65161 caacagggtc gtgttcgtaa agctgaacgc tggggaccac gcacgctgga tctcgatatc
    65221 atgctgtttg gtaatgaagt gataaatacc gaacgcctga ccgttccgca ctacgatatg
    65281 aagaatcgtg gatttatgct gtggccgttg tttgagattg caccggagct ggcgtttcct
    65341 gatggggaga cgttgcgtga agtcttacat acaagagcat ttgacaaatt aagcaaatgg
    65401 taatttattt atattacgaa tataaatatt ttctgaaaat catacatcct ctctttcctt
    65461 aataaaaatt ttagtttaaa catttttgca gaaacataat ataaatttcc gcagataaat
    65521 atcaatccca tcgccatacc ctaaccaatt gcacttgtga atttattttt gcttaatttt
    65581 gaattaagaa aatttaattt gttcaaaagc ccttaaagtg cacaataggc gtcctaatga
    65641 acaatggttg taaattttgg taacaaatat cattgctcat taatatctag catctgctta
    65701 taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat ggaattttat
    65761 tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat gctggcaatg
    65821 gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag tggtctggtt
    65881 gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct gatcctgctg
    65941 caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt tggcgctaaa
    66001 gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc aggtagtaca
    66061 gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct gaacaacgac
    66121 atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat tgatggttcc
    66181 actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc cctggatgca
    66241 acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt cgcagaccaa
    66301 acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta tcagtaagta
    66361 ttcaccaatt aaattgattg aatgaatata cagggaatag tgttaataat ttctattttt
    66421 atattattcc ctgttttaat taactctgtc agggatggtt tatgtttttt aacaccaaac
    66481 atacaacagc tttatgcttc gtaacctgta tggcttttag ttcatcgtct attgcggaca
    66541 ttgtcatttc gggtactcgc gtaatatata aaagcgatca aaaaagtgtc aacgtacgtc
    66601 tggaaaataa agggaataac ccgttgcttg tccagagttg gttagatact ggcgatgaca
    66661 acgctgagcc tggcagtatt acagtccctt ttactgccac gccgccagta tcgcgtattg
    66721 atgccaaacg tgggcaaaca atcaaattaa tgtacacagc cagcacctca ctgcctaaag
    66781 acagagagag cgtgttctgg tttaacgtac tggaagttcc accaaaacca gatgcagaaa
    66841 aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac acgtataaaa cttttctatc
    66901 gcccggatgg attgaaggga aatccctctg aagccccgtt agccctgaag tggttctggt
    66961 caggttcaga aggtaaggcg tcattgcgag tgaccaatcc aaccccttac tacgtctctt
    67021 ttagcagtgg tgatttagaa gctagcggta aacgctatcc gattgatgtg aaaatgattg
    67081 caccatttag tgatgaggtc atgaaagtca ctggccttaa tggcaaagca agttctgcaa
    67141 aagtacattt ttacgccatt aatgattttg gtggcgccat tgaaggtaat gccagtctgt
    67201 aatcaggcaa ggatataatt ccgcaggaag cataacgtga ctataaaacc aactaaaaat
    67261 tatcataatc atctgacccg tatcgctacg ttttgcgcgc tgctgtattg caatagtgct
    67321 ttctgtgctg aacttgttga atatgaccat accttcctga tggggcagaa tgcctcaaat
    67381 attgatctca gccggtatag tgaaggtaac cctgctatac cgggtatgta cgacgtcagt
    67441 gtttatgtca acgatcaacc tattattaac caaagcatta catttattga aattgaagga
    67501 aaaaaaaacg cacaagcctg tatcacactt aagaatttat tgcagtttca tataaattct
    67561 cccgatataa ataacgaaaa agccgttctg cttgccaggg atgaaacact cggcaattgc
    67621 cttaatttga cggaaattat ccctcaggct tctgttcgtt atgacgttaa cgagcaacgt
    67681 ctggatatag acgttcctca ggcatgggta atgaaaaatt accaaaacta tgttgatcca
    67741 tcgttatggg aaaatggcat taatgcggcc atgttgtcat acaacctcaa cggatatcat
    67801 agtgaaactc ctggtcgaag aaatgacagc atttatgctg cttttaacgg tgggatgaat
    67861 ttaggtgcat ggcgactgcg tgcctcaggc aactacaact ggatgaccga ttctggcagt
    67921 aattatgatt ttaagaatcg gtatatacag cgtgatatcg cctcgctgcg ttctcaactc
    67981 attcttggtg agtcttatac aacgggcgaa acctttgatt ctgtcagtat ccgcggcatt
    68041 cgtttataca gcgacagccg catgttgcct ccgacattag cgagttttgc gcctatcatt
    68101 catggcgttg ctaataccaa cgccaaagtc actattacgc aaggtggcta taagatttat
    68161 gaaacgactg tgccgccagg cgcgttcgtc attgatgatc tgagtccgtc aggttacggc
    68221 agcgatctta ttgttactgt cgaagaatcc gatggctcaa agcggacatt ctcgcaacct
    68281 ttttcatcgg tcgttcaaat gttacgccct ggcgttggac gttgggatat tagcggcggc
    68341 caggtcttaa aagatgatat tcaggatgag cctaatttat ttcaggccag ctactactac
    68401 ggtctgaata actatctaac gggttatact ggtattcaga ttaccgataa taactatacc
    68461 gctggtttgt taggtcttgg tctgaatact tcagtcggtg cattttcttt cgatgtgact
    68521 cactccaatg ttcgtatacc ggatgataaa acatatcagg gacaaagtta tcgtgtttcc
    68581 tggaacaagt tattcgaaga aacaagtact tcactgaata tcgcggccta tcgctattcg
    68641 acacagaatt accttggtct taatgatgca ctaactctaa ttgatgaagt gaaacatccc
    68701 gaacaagatc ttgaaccgaa atccatgcgt aattactcgc gcatgaaaaa tcaggttacg
    68761 gtcagtatta accaaccgct aaaatttgag aaaaaagatt acggttcatt ttatctttcc
    68821 ggaagttggt ccgattactg ggcttccgga caaaatcgta gcaattactc tattggctac
    68881 agtaacagtg catcctgggg cagctacagt gtcagtgccc agcgttcatg gaatgaagac
    68941 ggcgacactg acgatagcgt ttatctcagt ttcaccattc ctattgaaaa attacttggc
    69001 actgaacaac ggacttcagg tttccagagt attgatactc aaatgagcag tgactttaag
    69061 ggtaataacc aactcaacgt cagtagcagt ggctatagtg ataacgctcg cgtcagttat
    69121 agcgtaaata ctggctatac gatgaataaa gccagcaaag atttgagtta tgttgggggt
    69181 tatgccagct atgaatcacc gtggggaacc ctggcaggtt cagtttctgc aaatagcgat
    69241 aacagtcgtc aagtgtctct cagcaccgac ggtggttttg tattgcatag cggcggcctg
    69301 accttcagta atgacagttt tagcgactcc gatacactgg cggtagttca ggctccaggt
    69361 gctcaaggag cgcgaataaa ttatggcaac agtactatcg atcgatgggg ttatggtgtc
    69421 accagcgctc tttctcctta tcatgaaaac cgtatcgcgc tggatatcaa cgatcttgag
    69481 aacgatgttg aattaaaaag taccagtgca gtagctgtac cgcgtcaggg ttcagtcgtc
    69541 tttgctgatt ttgaaaccgt gcaagggcaa tcagccatta tgaacatcac acgaagtgat
    69601 ggtaaaaata ttccatttgc tgcagatatt tatgatgagc aaggcaatgt cattggtaat
    69661 gttggacagg gtggacaagc atttgttcgt ggtattgagc agcagggaaa tatcagcatt
    69721 aaatggctcg aagaaagtaa acccgtaagt tgtcttgcgc attatcaaca aagttcagaa
    69781 gcagaaaaaa tagcgcaatc tattattctg aatggaattc ggtgtcagat tcagtaacta
    69841 caagggacgt caaatgataa aaataacgcc acataaaata gcgatactga tgggaatatt
    69901 attatcacca tcagtatttg caacggatat taatgtagag tttacagcca ctgtcaaagc
    69961 gacaacctgt aacatcacac ttactggtaa taacgtcacg aatgatggca ataataacta
    70021 cacattgaga atccctaaga tgggtctgga taagatcgcg aataaaacga cagaatctca
    70081 ggctgatttt aaactggttg ctagtgggtg cagcagtggc atcagttgga ttgataccac
    70141 tctgaccgga aatgcatcat caagctcacc taagcttatt ataccgcagt ctggtgattc
    70201 atcttcgacg acaagtaata tcggtatggg tttcaaaaaa cggactactg atgatgccac
    70261 tttccttaaa cctaacagtg cggaaaagat acgctggagc acagacgaga tgcagcccga
    70321 taagggtctt gaaatgaccg ttgcgctacg tgaaacagat gcagggcaag gcgtaccggg
    70381 gaattttcgt gcactggcaa cgtttaattt catctatcaa taagaaggat aattaagatg
    70441 acatttaaaa atttacgtta tggattatcc agcagtgtcg ttttggctgc ctcattgttc
    70501 agcgtaccct cttatgcggc aacagacagc attgggctga ccgtgattac taccgtagaa
    70561 ataggtactt gtactgctac attagtaaat gactctgatc aggacatttc tgttgttgac
    70621 tttggcgatg tatatatttc tgaaatcaat gccaagacca aagttaaaac attcaaactc
    70681 aaattcaaag actgtgcggg tatccccaat aaaaaagcac aaataaaatt aaccaagcga
    70741 gccacatgcg aaggtactgc taatgacggt gcggggttcg caaatggatc cacagccgca
    70801 gataaagcaa gtgccgtcgc cgttgaagtc tggagcaccg aaactccggc aacaggtagc
    70861 gcaacacaat ttagttgtgt gacaccagca tctcaagagg tcactatctc caatgcagcc
    70921 aacgcggttg tttattatcc gatgagtgct cgactggtcg tcgaaaaaaa taaaaccgta
    70981 agcaatgtca ctgcgggtaa attttctgca cctgccacat ttacagtaac ctataactaa
    71041 cagactgaca gatccaggaa ctatatatgc atcccactca acgtaagctg atgaagagaa
    71101 taattctgtt tctgtcacta cagtttccca ttacctatcc agccatcgct gggcaggata
    71161 ttgaccttgt tgccaatgta aaaaacagca cttgcaaaag cggaatcagt aaccagggta
    71221 atattgatct tggcgtcgtt ggggtgggat atttttcaga taatgttact cctgaaagct
    71281 atcaaccagg tggaaaagag ttcactgtca cagtatcaga ctgtgcatta cagggaactg
    71341 gcgatgtgct taatcagtta catattgatt ttagagccct tagcggtgtc atggctgcag
    71401 gttcaaggca aatatttgcc aatgaagtcg caaccggggc taaaaatgtc ggtgtcgttc
    71461 tcttttccat tcaggacccg accaatatat tcaatgttat tagttcagca ggaaattctc
    71521 gttccgtgta tccagtaatg acttctgccc tgcataattc ttcatggaaa ttttatgcca
    71581 gaatgcaaaa aatcgatccc gcgctggatg tcatatctgg ccaggtaatg agtcatatat
    71641 tagttgatgt gtactacgaa taatagatca aaacaaataa ctgcgactgt tacagcagta
    71701 gatggtggag tgataatgaa gaaattcttc agacattttc tttttttaat tctgtgcttg
    71761 agttgttata ccgcgagtgc tggcactgat gataacgtag gatatattgt tgggaatagt
    71821 tacggcgttg gtccgtcaga tcaaaaatgg agagaaacgg ggccgaatgg cgatgctact
    71881 gtcatattca gatatgcaac aagtacaaat aatttagttt tctacaaacc gacgcagctt
    71941 ggacctacag gtgtaaaatt acagtggagt cagttagata ccgcttctgg tggtggtttt
    72001 ctttattgca acagaagtga tagcacaagt ggtagcgcaa tgcgtattga aaatgcaatg
    72061 gttgactcag gtaaaatgta tggctcccat aaattattta atacatcagt tcctggtttg
    72121 tattacacat tattaatttc aaacatgtgg tcagcttacg gtaccgtaac taacgttagt
    72181 tcacctggga tatacattgg tgactctgca gaacaatatt tttcgtggta taatccaagc
    72241 gaagacgtgt tatattggag ttgcaataat gcgaatagca cccgtaaata ctgggctgta
    72301 ggtggtattt atcagaccct tacaattgaa ttctatacag atacaaactt tgatccaaca
    72361 gtaactcaac agatcaaatt atccagctca tcaaattatc tgtattcatt taaagcctat
    72421 ggcgcaggcc aaggtataaa tgagcatagt tattttatca aaatcgattt cgatctgctc
    72481 aatgtcaagt tgactaatcc cacttgtttt accgctatgc ttagtggaac ttctgtgact
    72541 ggttctacgg taaaaatggg tgaatatagt gcagaacaaa tcagaaacgg tgccacaccg
    72601 gttccttttg atatttcact tcaaaactgc gttcgtgtga ctaatattga gacaaaatta
    72661 gtttcaacaa aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat
    72721 gacgcagcaa aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta
    72781 atgacattga aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa
    72841 gacgatacaa caggaggagt ttacccggat caagatacag gtataacata ccctctccat
    72901 ttccaggcca cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc
    72961 accagtactt tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg
    73021 tgcggtaatt gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg
    73081 acatcattta tcaggacacg ttatgaaacc gaccaccatt gcttcacttc agaaatgtaa
    73141 gcaggataaa aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt
    73201 tgctgatgaa ggacttaacg tcatgctggt aggcgattcg ctgggcatga cggttcaggg
    73261 ccacgactcc acccttcccg ttaccgtcga ggatatcgcc tatcataccg ccgccgtacg
    73321 tcgcggcgcg ccaaactgcc tgctgttggc tgacctgccg tttatggcgt atgccacgcc
    73381 ggaacaagcc tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat
    73441 tgagggcggt gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt
    73501 atgtggtcac ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagttca
    73561 ggggcgcggc gatgaagcgg gcgatcaact gctcagcgat gcattagcct tagaagccgc
    73621 tggggcacag ctgctggtgc tggaatgcgt gccggttgaa ctggcaaaac gtattaccga
    73681 agcactggcg atcccggtta ttggcattgg cgcaggcaac gtcactgacg ggcagatcct
    73741 cgtgatgcac gacgccttcg gcattaccgg cggtcacatt cctaaattcg ctaaaaattt
    73801 cctcgccgaa acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc
    73861 cggcgtttat ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc
    73921 gaaaccctgc cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg
    73981 gcgctggtgc ctactatggg taatctgcac gatggccata tgaagctggt cgacgaagcc
    74041 aaagcccgcg ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc
    74101 ccggaagatc tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa
    74161 cgtaaagtgg atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa
    74221 acccatactt acgttgacgt tcccgggctt tcgaccatgc tggaaggcgc gagccgtccg
    74281 gggcattttc gcggcgtttc gactatcgtc agcaagctgt ttaacctggt ccagccggac
    74341 atcgcctgct tcggtgaaaa ggactttcag caactggcgc tgatccgcaa aatggttgcc
    74401 gatatgggct tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agatggcctt
    74461 gcgctcagtt cccgtaatgg ttatctgacg gcggaacaac gcaaaattgc gcccggtctg
    74521 tacaaagttt taagttcaat tgccgacaaa ttgcaggctg gggaacggga tctcgatgaa
    74581 attattacca ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag
    74641 attcgcgatg ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg
    74701 gtagccgcct ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa
    74761 tacttaactg gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca
    74821 taacacaggt aacttaaata aggttaatta ccttatcaat ctcagcaaga gataaatttg
    74881 tcatctctgc aacgtctttc cgagacattc ctttacttag aagacgcagg gcgaattctt
    74941 gcctttcctc ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact
    75001 gcgccagcgt catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca
    75061 aatccgcttg ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac
    75121 ttccgctagt gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt
    75181 cgcgctggcg aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga
    75241 tttcgtcatc cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc
    75301 gtgccagctc cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg
    75361 cctcgccctg ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca
    75421 gatgccggtg catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg
    75481 gcttgctttg gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg
    75541 aatagagcac gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta
    75601 aatgaagcgt gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa
    75661 agtcgcgagc cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg
    75721 tactcggtgc atccatcttc cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt
    75781 caaggcgcgc cgtcgtcagc cgcctcttta gtgtttaccg agcagcgttc agagaaattt
    75841 caattcgtta caggcgatac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa
    75901 tgccatcaga tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca
    75961 aagcaggcac cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac
    76021 gatgctgcag ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg
    76081 ttcttgcgcc attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat
    76141 cgatatctgg aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg
    76201 cggttcgaga attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat
    76261 tgtcatcatc gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa
    76321 tgtcgcctat tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca
    76381 ggttgcttga ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg
    76441 aatccgttct taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt
    76501 tgcctttcat ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt
    76561 tgtcattaaa tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca
    76621 gagcgcggcg tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata
    76681 tgggtcggcg atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa
    76741 catcgcgaat ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact
    76801 tctgcgggtg acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt
    76861 gtttcaatac aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca
    76921 ccaccgctcg cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc
    76981 tcagtgtcgc gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg
    77041 aggtggtcgt cgaagtatgg cgaaaacggg tgttttcttc atcgcgcaga atatggtgat
    77101 aggttagcac cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggcgagtc
    77161 gatcgccaat acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa
    77221 tcgggtagcg caaattgtcc gccagtaccc caaatggcgc actgcccgca ctcggcgcgt
    77281 tatagaccgg cgtatctttc caggtaatta agttctgatt actcagcggc ttgttgaggt
    77341 cacccaaacc gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga
    77401 taaacccttt accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg
    77461 caatgatttg cccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc
    77521 agatcgcagc attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg
    77581 cggtaaacag catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg
    77641 tgaaacaacg cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca
    77701 aattatgttt gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct
    77761 ccggcccacc cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc
    77821 aagccatcat ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt
    77881 gtttgtggct taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga
    77941 agatgacacc ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca
    78001 tattagagct taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt
    78061 ttttctccag cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac
    78121 ctaacatcgc cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg
    78181 tgatagccgt caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg
    78241 caaaccacgt ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac
    78301 caccagtacg ccaaaagtag tgaccagcgg aacatcattg ataccgagga agccgtagcg
    78361 gaaaccactg atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg
    78421 caacaaagtc agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt
    78481 tggcaccagg ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag
    78541 ggagaacaac accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg
    78601 aaacggcaca aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc
    78661 cacgccaccg ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag
    78721 ctcttcaata ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc
    78781 attggtgatc accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc
    78841 atgcatatcg ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat
    78901 ggtgatgact ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt
    78961 cgcccagatg ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt
    79021 tcattaacca gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat
    79081 acctgaatgc cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc
    79141 acttcaactt ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt
    79201 aacggacttt tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc
    79261 gccttcatcg aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac
    79321 agcatttctg cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt
    79381 aaatccttca aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt
    79441 tcgtcgagaa tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc
    79501 ttcatcccgc cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt
    79561 ttaagatatt tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc
    79621 tgattcacca caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc
    79681 agtcccaact gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg
    79741 acccgcccgg aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg
    79801 gccccgttcg gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca
    79861 cgaagcgcct gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg
    79921 gtcataaatt tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg
    79981 agttgcccta tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag
    80041 acatagatac actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc
    80101 ccgggttttt tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt
    80161 ccgacagtcg cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc
    80221 accgtaatgt tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt
    80281 atgcagtgga tgtactagaa gttgaacaca ttattatctg tggccactac ggttgcggcg
    80341 gcgtacaagc cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata
    80401 tccgcgatat ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc
    80461 tggatacctt gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca
    80521 ttatgcaatc agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctatggca
    80581 ttcacgacgg cttgctgcgt gatctggatg tcacagcgac taaccgcgaa acccttgagc
    80641 aacgttaccg tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat
    80701 gccatgccgg atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt
    80761 gccgatatac ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa
    80821 ctcatccggg atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg
    80881 tttatccagc agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg
    80941 cactttcgac agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc
    81001 acggatatct tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct
    81061 accgtagctg gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag
    81121 atccgccata aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct
    81181 gtctttgtaa cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc
    81241 ttcggggatc attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa
    81301 atcacttcgc acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag
    81361 taaaactatt cattcgcgga atctgttaat aaagctcgaa ttgtgatgac gatcacacat
    81421 gttaaaacct ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat
    81481 ggcaattaac aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc
    81541 gctttgcggg ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg
    81601 gtactaccct atcgctggtc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa
    81661 acgtgccgcg ctttggctgt acgcagccct gctgctcggc accatgattt ggggcgtctg
    81721 ggaagttggt ttcgacttct gggcgctgac tccgcgcagc gacattctgg tcttcttcgg
    81781 catctggctg atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt
    81841 tgccgcactg gtggtggcac tgctgattag cggtggtatc ctgacctggg ccggatttaa
    81901 cgatccgcag gaaatcaacg gcaccttaag cgccaatgcc acacctgcgg aagctatctc
    81961 acccgttgcc gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc
    82021 acctctgaaa caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg
    82081 tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat
    82141 taaagtgggc gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc
    82201 cgccagcggc aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca
    82261 gcacgtaacc tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga
    82321 agtgatggcg gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc
    82381 gattaacgct gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct
    82441 gcaaagcaat atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat
    82501 caccgataaa accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac
    82561 gtctggcgtg atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc
    82621 cggcgcgaaa gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc
    82681 aaactcctgg gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg
    82741 cgtgaccacg ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag
    82801 ctcgattctg gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca
    82861 ccacgacctg tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa
    82921 tggtcagaaa gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga
    82981 tcgtcgcaat ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc
    83041 gaaaggcgat tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa
    83101 agatttgagc ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt
    83161 gatgttccac cagttgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct
    83221 ggtcttcccg ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg
    83281 tgaagtggca attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg
    83341 tccgggtaac ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat
    83401 tcagccacag tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg
    83461 tctgccgtgt aaacagccag catggggtta tatctcggcg ctggacctga aaaccaatga
    83521 agtggtgtgg aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt
    83581 accggtgccg ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa
    83641 cgtgctgttt atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg
    83701 tgaaaaactg tggcagggtc gtttaccagc gggtggtcag gctacgccaa tgacctatga
    83761 agtgaatggc aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa
    83821 gatgggcgac tatattgtgg cttatgcgct gccggatgat gtgaaataac cataatgccc
    83881 ggagagatcc gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc
    83941 cccgtatctt catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg
    84001 ttgtgattaa acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc
    84061 cagcccgcgc gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgggtg
    84121 ccgtgaatat ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg
    84181 tactgccctt tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt
    84241 ttattggcat ggtggaaatc gaacttcccg ccatggttca tatgattcat attgccgtgc
    84301 cccatatggc ccatcatctg gctatgatcc atcccggcca tcgcctgatc gccatatttc
    84361 tccatcagca tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg
    84421 cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa
    84481 gcaccggagg cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac
    84541 ggcgcaatcg ccatccccat ctggctgacc ggtagcgtca ccaggtcaaa gggtttgttg
    84601 tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc
    84661 ttcaccggtt caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg
    84721 ctggtggcga aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa
    84781 ccacgtgggg cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac
    84841 cagcccacgg cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat
    84901 tttttatcct gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat
    84961 ttcaggatct cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc
    85021 ccggtcttgc catgttgatg tggatggaac cagcaagtag cggcaggttg atcaacgttc
    85081 aacgtcaccg agcgcttgcc acctggcgga ataattccct gcgggccgcc gtcgacttca
    85141 cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt ccgtcagttg gttatagata
    85201 tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta
    85261 tagccccagg tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac
    85321 tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc
    85381 gccgcaaaga ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa
    85441 tattttaaga aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa
    85501 atcaaacgtt gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa
    85561 tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc
    85621 tggtagaagc aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt
    85681 gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag
    85741 tttttgtctt tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc
    85801 gcgcactggt ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg
    85861 acatgaaagc tctcggtgaa gacagctacc gcgatctcag cggcattggc attcctgtcg
    85921 ctaaaaaatg caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat
    85981 ccctgatacc tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg
    86041 ttgcgccctt tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac
    86101 agtggcatga aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt
    86161 atcatgaaaa gaccgatcac caggatctga tcatttttga gaacgctgca tttggtcgcg
    86221 taatggcgct ggatggcgta gtacaaacca ccgagcacga cgagtttatc tatcatgaga
    86281 tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg
    86341 gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga
    86401 tggtggaaat cgatgcgggt gtcgtatcgt tctgccgtca gtatctaccc aaccataacg
    86461 ccggtagcta cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta
    86521 atcaaaccag ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg
    86581 gcgaaagcct tttcacttcg gcattttatg aaggctgcaa acgttgcctg aatcctggcg
    86641 gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc
    86701 atcgcaaact cagccattac ttcagcgacg ttggctttta tcaggcggcg attccgacct
    86761 attacggcgg tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct
    86821 caaccgaaat tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc
    86881 cggcaatcca tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac
    86941 agccgtccta aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc
    87001 tgaccaaaag tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc
    87061 gcgacggtta tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc
    87121 tgtcagaaac ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg
    87181 aaccacaggg tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca
    87241 tcgacaaaac agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa
    87301 gtcatatttg cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc
    87361 gcgccgatat tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc
    87421 tgatccacca gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc
    87481 gcgacattaa cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta
    87541 tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata
    87601 tcttccatac caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca
    87661 aaccggaaga tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa
    87721 tgcgcgagat ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc
    87781 aggctccgcc agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg
    87841 cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc
    87901 ttccatttca tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc
    87961 catccacagg gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc
    88021 gtgcgctgct tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa
    88081 ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc
    88141 gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca
    88201 tcgcgccatt gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt
    88261 ttagaaataa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
    88321 gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta
    88381 ctgagaaagc tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg
    88441 cgcaacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
    88501 cgccagttct gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt
    88561 acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca
    88621 cagagaacag cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc
    88681 ttcttcagtc aactgtgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg
    88741 caactggcct ttatgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt
    88801 catgcaagaa ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg
    88861 cgcgtcatcc gggtcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat
    88921 gtcaatcact gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag
    88981 ccatttttcc atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc
    89041 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
    89101 cagcttgata gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc
    89161 ctgctcaact ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt
    89221 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc
    89281 gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
    89341 ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga
    89401 acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcacctc cggtaccgac
    89461 ggtatccggc agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag
    89521 cgacacaccg ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
    89581 tggcttcgga tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc
    89641 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc
    89701 tacagaagtc attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc
    89761 acggcctacc attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
    89821 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc
    89881 acgcccgata atcagcggga tacggccgcc agcacgcact tcatcaatca gcacgtcggt
    89941 tttcagttcg aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta
    90001 cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
    90061 tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa
    90121 accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga
    90181 gttagtggcg gatttacgcg aagaacccgt acccacaacg tcaccgacgt acgccagcgg
    90241 gaaacctttc tgttgcagag cttcgatttg cttgatcgga ccaacaacac caggctggtc
    90301 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
    90361 gcgtgaccac gcatccggtg ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt
    90421 gaagacggta acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc
    90481 cgcccaggac tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac
    90541 gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc
    90601 cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt
    90661 gcccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttcgc
    90721 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg
    90781 gttggttaac agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
    90841 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
    90901 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
    90961 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
    91021 agtaccgcaa cgggcagaat agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag
    91081 caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
    91141 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
    91201 tttaaactac cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac
    91261 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
    91321 ctggcgctaa tttgcagtgc cgggctttgt gccgcctcta ccgggctata tataaaaagc
    91381 cgcacagtgg aagcgcctgt ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc
    91441 gcagttacgc ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc
    91501 gcattcagca ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct
    91561 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa
    91621 tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcagt atctgaccag
    91681 ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc
    91741 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    91801 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca
    91861 accgaacaga aagttattac cacaacgaag aaagctgtac cggtaacaca gacagtcacg
    91921 gctcccgtca taccatccaa taccgtttca actgccagcc ccatcattac agagcctgca
    91981 acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac
    92041 ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca
    92101 ccagccggag aaccgtttat tgacagcttt gtgcgcggat tcggctatag catgggtgtt
    92161 gctaccacgt acgcactatt cagcagcatc gactgggacg acgacgatca tgaccatcat
    92221 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg
    92281 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    92341 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    92401 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt
    92461 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    92521 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    92581 cagcggttta atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt
    92641 cgccaacccc tgacccagca acaaaaggac gccgctcgtc agcgttatca gtccgcctcg
    92701 cctgagcagc gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga
    92761 agagaggcag cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtatttaag
    92821 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata
    92881 caatcggcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    92941 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta
    93001 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    93061 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aacaattact
    93121 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    93181 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    93241 cacaacccat ttcgattgcc aggccgattt cacccagcag ctcgccgccg ttggtaccga
    93301 caatcgcacc cccgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    93361 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    93421 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    93481 cggtataggc gatggacggg ataactttcg gatcgaagta gtgtttctta ccggcgataa
    93541 cttcagcggc aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga
    93601 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    93661 aaccacggtc gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    93721 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    93781 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    93841 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    93901 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    93961 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    94021 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    94081 acggcagttg gatcgggcga gaacccgctg caatgatcgc gttgtcgaag ttgatcacgg
    94141 ttttaccgtt ctcaccttca acttccaggg tgttagcccc ggtaaattta cccagaccgt
    94201 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    94261 tcactttctc tttccaggta cgaatcttgt caatatcggt tttcggttcg ccgaagacga
    94321 taccgtgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    94381 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    94441 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    94501 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    94561 ctccggcggt catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc
    94621 ccagtcactt acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca
    94681 gaaatccata gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg
    94741 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    94801 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    94861 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    94921 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    94981 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    95041 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    95101 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    95161 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    95221 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    95281 atttcttcag ggtcagacgc tgaccgtctt ccgacaacga actgttgaag cgaggcatct
    95341 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    95401 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    95461 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    95521 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    95581 ccaccttcgg ccacggcagc atgcctggga taccgccgcc agtcgccgcc ggagctgctt
    95641 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    95701 tacggccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    95761 tcagcggagt cgcgtgaacg taagcgtcgt tttctgcaaa ttcagatttg ccttccgctt
    95821 tcgcagccgg tgctgctgcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    95881 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    95941 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    96001 gagccggaac ttccatagaa gctttgtcgc cttctacggt aatcagtgac tgttcagcgg
    96061 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    96121 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    96181 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    96241 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    96301 acggagccgg aacttccata gacgctttat cgccttctac ggtgatcagc gactgttcag
    96361 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    96421 tatccggaac gttaacgtct tttgccgctg cagccgctgg tgctgctgcc ggagctgctt
    96481 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    96541 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    96601 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    96661 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    96721 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    96781 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    96841 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gctacaacca cgtaggaagc
    96901 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    96961 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    97021 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    97081 gcgcggagtt tccagcgggt gcagcatgtt ccagcgttca caatcctgac catctcgcgc
    97141 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    97201 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
    97261 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    97321 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    97381 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    97441 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    97501 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    97561 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    97621 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
    97681 agaggtcgcc gctgccagcc aggaacaacc tgcgcccaac tcgttgatcc cttcctgcag
    97741 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    97801 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    97861 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    97921 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    97981 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    98041 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
    98101 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    98161 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    98221 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    98281 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    98341 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    98401 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    98461 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    98521 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    98581 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    98641 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    98701 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    98761 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    98821 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
    98881 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    98941 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
    99001 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    99061 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    99121 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    99181 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    99241 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    99301 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    99361 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    99421 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    99481 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    99541 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    99601 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    99661 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    99721 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    99781 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    99841 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    99901 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    99961 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
   100021 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
   100081 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
   100141 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
   100201 cctcacgcaa ggagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
   100261 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
   100321 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
   100381 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
   100441 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
   100501 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
   100561 agtttttta
//
LOCUS       DAZFFI010000005        95840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFFI010000005 DAZFFI010000000
VERSION     DAZFFI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.005"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            590..2158
                     /gene="cydA"
                     /locus_tag="ACHJ12_000450"
     CDS             590..2158
                     /gene="cydA"
                     /locus_tag="ACHJ12_000450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4988297.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2174..3313
                     /gene="cydB"
                     /locus_tag="ACHJ12_000451"
     CDS             2174..3313
                     /gene="cydB"
                     /locus_tag="ACHJ12_000451"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4988298.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3328..3441
                     /gene="cydX"
                     /locus_tag="ACHJ12_000452"
     CDS             3328..3441
                     /gene="cydX"
                     /locus_tag="ACHJ12_000452"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4988299.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3441..3734
                     /gene="ybgE"
                     /locus_tag="ACHJ12_000453"
     CDS             3441..3734
                     /gene="ybgE"
                     /locus_tag="ACHJ12_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4988300.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3884..4288
                     /gene="ybgC"
                     /locus_tag="ACHJ12_000454"
     CDS             3884..4288
                     /gene="ybgC"
                     /locus_tag="ACHJ12_000454"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4988301.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4285..4977
                     /gene="tolQ"
                     /locus_tag="ACHJ12_000455"
     CDS             4285..4977
                     /gene="tolQ"
                     /locus_tag="ACHJ12_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4988302.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4981..5409
                     /gene="tolR"
                     /locus_tag="ACHJ12_000456"
     CDS             4981..5409
                     /gene="tolR"
                     /locus_tag="ACHJ12_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4988303.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5474..6739
                     /gene="tolA"
                     /locus_tag="ACHJ12_000457"
     CDS             5474..6739
                     /gene="tolA"
                     /locus_tag="ACHJ12_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4988304.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6872..8164
                     /gene="tolB"
                     /locus_tag="ACHJ12_000458"
     CDS             6872..8164
                     /gene="tolB"
                     /locus_tag="ACHJ12_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4988305.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8199..8720
                     /gene="pal"
                     /locus_tag="ACHJ12_000459"
     CDS             8199..8720
                     /gene="pal"
                     /locus_tag="ACHJ12_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4988306.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8730..9521
                     /gene="cpoB"
                     /locus_tag="ACHJ12_000460"
     CDS             8730..9521
                     /gene="cpoB"
                     /locus_tag="ACHJ12_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4988307.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9686..9761
                     /locus_tag="ACHJ12_000461"
     tRNA            9686..9761
                     /locus_tag="ACHJ12_000461"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9719..9721,aa:Lys,seq:ttt)
     gene            9797..9872
                     /locus_tag="ACHJ12_000462"
     tRNA            9797..9872
                     /locus_tag="ACHJ12_000462"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9830..9832,aa:Val,seq:tac)
     gene            9875..9950
                     /locus_tag="ACHJ12_000463"
     tRNA            9875..9950
                     /locus_tag="ACHJ12_000463"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9908..9910,aa:Lys,seq:ttt)
     gene            10100..10175
                     /locus_tag="ACHJ12_000464"
     tRNA            10100..10175
                     /locus_tag="ACHJ12_000464"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10133..10135,aa:Val,seq:tac)
     gene            10179..10254
                     /locus_tag="ACHJ12_000465"
     tRNA            10179..10254
                     /locus_tag="ACHJ12_000465"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10212..10214,aa:Lys,seq:ttt)
     gene            10401..10476
                     /locus_tag="ACHJ12_000466"
     tRNA            10401..10476
                     /locus_tag="ACHJ12_000466"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10434..10436,aa:Lys,seq:ttt)
     gene            10507..10632
                     /locus_tag="ACHJ12_000467"
     ncRNA           10507..10632
                     /ncRNA_class="other"
                     /locus_tag="ACHJ12_000467"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10881..11924
                     /gene="nadA"
                     /locus_tag="ACHJ12_000468"
     CDS             10881..11924
                     /gene="nadA"
                     /locus_tag="ACHJ12_000468"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4988308.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11962..12681
                     /gene="pnuC"
                     /locus_tag="ACHJ12_000469"
     CDS             11962..12681
                     /gene="pnuC"
                     /locus_tag="ACHJ12_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4988309.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12678..13619)
                     /gene="zitB"
                     /locus_tag="ACHJ12_000470"
     CDS             complement(12678..13619)
                     /gene="zitB"
                     /locus_tag="ACHJ12_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4988310.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13733..14113)
                     /gene="ybgS"
                     /locus_tag="ACHJ12_000471"
     CDS             complement(13733..14113)
                     /gene="ybgS"
                     /locus_tag="ACHJ12_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4988311.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14429..15481
                     /gene="aroG"
                     /locus_tag="ACHJ12_000472"
     CDS             14429..15481
                     /gene="aroG"
                     /locus_tag="ACHJ12_000472"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4988312.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15639..16391)
                     /gene="gpmA"
                     /locus_tag="ACHJ12_000473"
     CDS             complement(15639..16391)
                     /gene="gpmA"
                     /locus_tag="ACHJ12_000473"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4988313.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16596..17636)
                     /gene="galM"
                     /locus_tag="ACHJ12_000474"
     CDS             complement(16596..17636)
                     /gene="galM"
                     /locus_tag="ACHJ12_000474"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4988314.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17630..18778)
                     /gene="galK"
                     /locus_tag="ACHJ12_000475"
     CDS             complement(17630..18778)
                     /gene="galK"
                     /locus_tag="ACHJ12_000475"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4988315.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18782..19828)
                     /gene="galT"
                     /locus_tag="ACHJ12_000476"
     CDS             complement(18782..19828)
                     /gene="galT"
                     /locus_tag="ACHJ12_000476"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4988316.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19838..20854)
                     /gene="galE"
                     /locus_tag="ACHJ12_000477"
     CDS             complement(19838..20854)
                     /gene="galE"
                     /locus_tag="ACHJ12_000477"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4988317.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21115..22587)
                     /gene="modF"
                     /locus_tag="ACHJ12_000478"
     CDS             complement(21115..22587)
                     /gene="modF"
                     /locus_tag="ACHJ12_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4988318.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22655..23443)
                     /gene="modE"
                     /locus_tag="ACHJ12_000479"
     CDS             complement(22655..23443)
                     /gene="modE"
                     /locus_tag="ACHJ12_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4988319.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23572..23721
                     /gene="acrZ"
                     /locus_tag="ACHJ12_000480"
     CDS             23572..23721
                     /gene="acrZ"
                     /locus_tag="ACHJ12_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4988320.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23888..24661
                     /gene="modA"
                     /locus_tag="ACHJ12_000481"
     CDS             23888..24661
                     /gene="modA"
                     /locus_tag="ACHJ12_000481"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4988321.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24661..25350
                     /gene="modB"
                     /locus_tag="ACHJ12_000482"
     CDS             24661..25350
                     /gene="modB"
                     /locus_tag="ACHJ12_000482"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4988322.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25353..26411
                     /gene="modC"
                     /locus_tag="ACHJ12_000483"
     CDS             25353..26411
                     /gene="modC"
                     /locus_tag="ACHJ12_000483"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4988323.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26412..27230)
                     /gene="ybhA"
                     /locus_tag="ACHJ12_000484"
     CDS             complement(26412..27230)
                     /gene="ybhA"
                     /locus_tag="ACHJ12_000484"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4988324.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27385..28380
                     /gene="pgl"
                     /locus_tag="ACHJ12_000485"
     CDS             27385..28380
                     /gene="pgl"
                     /locus_tag="ACHJ12_000485"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4988325.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSLDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28421..29374)
                     /gene="ybhD"
                     /locus_tag="ACHJ12_000486"
     CDS             complement(28421..29374)
                     /gene="ybhD"
                     /locus_tag="ACHJ12_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4988326.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29558..30610
                     /gene="ybhH"
                     /locus_tag="ACHJ12_000487"
     CDS             29558..30610
                     /gene="ybhH"
                     /locus_tag="ACHJ12_000487"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4988327.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAGTSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30686..32119
                     /gene="ybhI"
                     /locus_tag="ACHJ12_000488"
     CDS             30686..32119
                     /gene="ybhI"
                     /locus_tag="ACHJ12_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4988328.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32302..34563
                     /gene="ybhJ"
                     /locus_tag="ACHJ12_000489"
     CDS             32302..34563
                     /gene="ybhJ"
                     /locus_tag="ACHJ12_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4988329.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34797..36080)
                     /gene="ybhC"
                     /locus_tag="ACHJ12_000490"
     CDS             complement(34797..36080)
                     /gene="ybhC"
                     /locus_tag="ACHJ12_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4988330.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36232..36708)
                     /gene="ybhB"
                     /locus_tag="ACHJ12_000491"
     CDS             complement(36232..36708)
                     /gene="ybhB"
                     /locus_tag="ACHJ12_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4988331.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(36767..38056)
                     /gene="bioA"
                     /locus_tag="ACHJ12_000492"
     CDS             complement(36767..38056)
                     /gene="bioA"
                     /locus_tag="ACHJ12_000492"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4988332.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            38143..39183
                     /gene="bioB"
                     /locus_tag="ACHJ12_000493"
     CDS             38143..39183
                     /gene="bioB"
                     /locus_tag="ACHJ12_000493"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4988333.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39180..40334
                     /gene="bioF"
                     /locus_tag="ACHJ12_000494"
     CDS             39180..40334
                     /gene="bioF"
                     /locus_tag="ACHJ12_000494"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4988334.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            40321..41076
                     /gene="bioC"
                     /locus_tag="ACHJ12_000495"
     CDS             40321..41076
                     /gene="bioC"
                     /locus_tag="ACHJ12_000495"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4988335.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            41069..41746
                     /gene="bioD"
                     /locus_tag="ACHJ12_000496"
     CDS             41069..41746
                     /gene="bioD"
                     /locus_tag="ACHJ12_000496"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4988336.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            42325..44346
                     /gene="uvrB"
                     /locus_tag="ACHJ12_000497"
     CDS             42325..44346
                     /gene="uvrB"
                     /locus_tag="ACHJ12_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4988337.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(44538..45446)
                     /gene="yvcK"
                     /locus_tag="ACHJ12_000498"
     CDS             complement(44538..45446)
                     /gene="yvcK"
                     /locus_tag="ACHJ12_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4988338.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      45711..45855
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            45843..46832
                     /gene="moaA"
                     /locus_tag="ACHJ12_000499"
     CDS             45843..46832
                     /gene="moaA"
                     /locus_tag="ACHJ12_000499"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4988339.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            46854..47366
                     /gene="moaB"
                     /locus_tag="ACHJ12_000500"
     CDS             46854..47366
                     /gene="moaB"
                     /locus_tag="ACHJ12_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4988340.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            47369..47854
                     /gene="moaC"
                     /locus_tag="ACHJ12_000501"
     CDS             47369..47854
                     /gene="moaC"
                     /locus_tag="ACHJ12_000501"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4988341.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            47847..48092
                     /gene="moaD"
                     /locus_tag="ACHJ12_000502"
     CDS             47847..48092
                     /gene="moaD"
                     /locus_tag="ACHJ12_000502"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4988342.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            48094..48546
                     /gene="moaE"
                     /locus_tag="ACHJ12_000503"
     CDS             48094..48546
                     /gene="moaE"
                     /locus_tag="ACHJ12_000503"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4988343.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            48683..49387
                     /gene="ybhL"
                     /locus_tag="ACHJ12_000504"
     CDS             48683..49387
                     /gene="ybhL"
                     /locus_tag="ACHJ12_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4988344.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            49532..50236
                     /locus_tag="ACHJ12_000505"
     CDS             49532..50236
                     /locus_tag="ACHJ12_000505"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO4988345.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            50306..51019
                     /locus_tag="ACHJ12_000506"
     CDS             50306..51019
                     /locus_tag="ACHJ12_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4988346.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(51055..52011)
                     /gene="ybhN"
                     /locus_tag="ACHJ12_000507"
     CDS             complement(51055..52011)
                     /gene="ybhN"
                     /locus_tag="ACHJ12_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4988347.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(52011..53252)
                     /gene="clsB"
                     /locus_tag="ACHJ12_000508"
     CDS             complement(52011..53252)
                     /gene="clsB"
                     /locus_tag="ACHJ12_000508"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4988348.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANIIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(53249..54010)
                     /gene="ybhP"
                     /locus_tag="ACHJ12_000509"
     CDS             complement(53249..54010)
                     /gene="ybhP"
                     /locus_tag="ACHJ12_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4988349.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            54143..54553
                     /gene="ybhQ"
                     /locus_tag="ACHJ12_000510"
     CDS             54143..54553
                     /gene="ybhQ"
                     /locus_tag="ACHJ12_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4988350.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(54515..55621)
                     /gene="ybhR"
                     /locus_tag="ACHJ12_000511"
     CDS             complement(54515..55621)
                     /gene="ybhR"
                     /locus_tag="ACHJ12_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4988351.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(55632..56765)
                     /gene="ybhS"
                     /locus_tag="ACHJ12_000512"
     CDS             complement(55632..56765)
                     /gene="ybhS"
                     /locus_tag="ACHJ12_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4988352.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(56758..58494)
                     /gene="ybhF"
                     /locus_tag="ACHJ12_000513"
     CDS             complement(56758..58494)
                     /gene="ybhF"
                     /locus_tag="ACHJ12_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4988353.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(58487..59482)
                     /gene="hlyD"
                     /locus_tag="ACHJ12_000514"
     CDS             complement(58487..59482)
                     /gene="hlyD"
                     /locus_tag="ACHJ12_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4988354.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(59485..60156)
                     /gene="cecR"
                     /locus_tag="ACHJ12_000515"
     CDS             complement(59485..60156)
                     /gene="cecR"
                     /locus_tag="ACHJ12_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4988355.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            60385..61749
                     /gene="rhlE"
                     /locus_tag="ACHJ12_000516"
     CDS             60385..61749
                     /gene="rhlE"
                     /locus_tag="ACHJ12_000516"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4988356.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(61981..62463)
                     /gene="ybiA"
                     /locus_tag="ACHJ12_000517"
     CDS             complement(61981..62463)
                     /gene="ybiA"
                     /locus_tag="ACHJ12_000517"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO4988357.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            62583..64733
                     /gene="dinG"
                     /locus_tag="ACHJ12_000518"
     CDS             62583..64733
                     /gene="dinG"
                     /locus_tag="ACHJ12_000518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4988358.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            64761..65723
                     /gene="ybiB"
                     /locus_tag="ACHJ12_000519"
     CDS             64761..65723
                     /gene="ybiB"
                     /locus_tag="ACHJ12_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4988359.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            65864..66949
                     /gene="hcxB"
                     /locus_tag="ACHJ12_000520"
     CDS             65864..66949
                     /gene="hcxB"
                     /locus_tag="ACHJ12_000520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4988360.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGVAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            67016..67075
                     /gene="ybiE"
                     /locus_tag="ACHJ12_000521"
     CDS             67016..67075
                     /gene="ybiE"
                     /locus_tag="ACHJ12_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4988361.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(67178..67438)
                     /gene="ybiJ"
                     /locus_tag="ACHJ12_000522"
     CDS             complement(67178..67438)
                     /gene="ybiJ"
                     /locus_tag="ACHJ12_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4988362.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(67703..67969)
                     /gene="ybiI"
                     /locus_tag="ACHJ12_000523"
     CDS             complement(67703..67969)
                     /gene="ybiI"
                     /locus_tag="ACHJ12_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4988363.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(68043..68720)
                     /gene="ybiX"
                     /locus_tag="ACHJ12_000524"
     CDS             complement(68043..68720)
                     /gene="ybiX"
                     /locus_tag="ACHJ12_000524"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4988364.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(68762..71044)
                     /gene="fiu"
                     /locus_tag="ACHJ12_000525"
     CDS             complement(68762..71044)
                     /gene="fiu"
                     /locus_tag="ACHJ12_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4988365.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(71309..71569)
                     /gene="mcbA"
                     /locus_tag="ACHJ12_000526"
     CDS             complement(71309..71569)
                     /gene="mcbA"
                     /locus_tag="ACHJ12_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4988366.1"
                     /translation="MKKCLTLLIATVLSGIFLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            71845..72771
                     /gene="rlmF"
                     /locus_tag="ACHJ12_000527"
     CDS             71845..72771
                     /gene="rlmF"
                     /locus_tag="ACHJ12_000527"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4988367.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDVLNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(72768..74993)
                     /gene="ybiO"
                     /locus_tag="ACHJ12_000528"
     CDS             complement(72768..74993)
                     /gene="ybiO"
                     /locus_tag="ACHJ12_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4988368.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(75110..75832)
                     /gene="glnQ"
                     /locus_tag="ACHJ12_000529"
     CDS             complement(75110..75832)
                     /gene="glnQ"
                     /locus_tag="ACHJ12_000529"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4988369.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(75829..76488)
                     /gene="glnP"
                     /locus_tag="ACHJ12_000530"
     CDS             complement(75829..76488)
                     /gene="glnP"
                     /locus_tag="ACHJ12_000530"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4988370.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(76627..77373)
                     /gene="glnH"
                     /locus_tag="ACHJ12_000531"
     CDS             complement(76627..77373)
                     /gene="glnH"
                     /locus_tag="ACHJ12_000531"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4988371.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(77777..78280)
                     /gene="dps"
                     /locus_tag="ACHJ12_000532"
                     /gene_synonym="pexB"
     CDS             complement(77777..78280)
                     /gene="dps"
                     /locus_tag="ACHJ12_000532"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4988372.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(78579..79466)
                     /gene="rhtA"
                     /locus_tag="ACHJ12_000533"
     CDS             complement(78579..79466)
                     /gene="rhtA"
                     /locus_tag="ACHJ12_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4988373.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            79701..79766
                     /locus_tag="ACHJ12_000534"
     CDS             79701..79766
                     /locus_tag="ACHJ12_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988374.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            79819..80334
                     /gene="ompX"
                     /locus_tag="ACHJ12_000535"
     CDS             79819..80334
                     /gene="ompX"
                     /locus_tag="ACHJ12_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4988375.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(80383..81966)
                     /gene="opgE"
                     /locus_tag="ACHJ12_000536"
     CDS             complement(80383..81966)
                     /gene="opgE"
                     /locus_tag="ACHJ12_000536"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4988376.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMKEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(82238..82366)
                     /gene="mntS"
                     /locus_tag="ACHJ12_000537"
     CDS             complement(82238..82366)
                     /gene="mntS"
                     /locus_tag="ACHJ12_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4988377.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            82552..83019
                     /gene="mntR"
                     /locus_tag="ACHJ12_000538"
     CDS             82552..83019
                     /gene="mntR"
                     /locus_tag="ACHJ12_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4988378.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            83016..84134
                     /gene="ybiR"
                     /locus_tag="ACHJ12_000539"
     CDS             83016..84134
                     /gene="ybiR"
                     /locus_tag="ACHJ12_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4988379.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTAIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(84193..85113)
                     /gene="ldtB"
                     /locus_tag="ACHJ12_000540"
     CDS             complement(84193..85113)
                     /gene="ldtB"
                     /locus_tag="ACHJ12_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4988380.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            85332..86924
                     /gene="ybiT"
                     /locus_tag="ACHJ12_000541"
     CDS             85332..86924
                     /gene="ybiT"
                     /locus_tag="ACHJ12_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4988381.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(87166..88431)
                     /gene="ybiU"
                     /locus_tag="ACHJ12_000542"
     CDS             complement(87166..88431)
                     /gene="ybiU"
                     /locus_tag="ACHJ12_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4988382.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(88583..89398)
                     /gene="ybiV"
                     /locus_tag="ACHJ12_000543"
     CDS             complement(88583..89398)
                     /gene="ybiV"
                     /locus_tag="ACHJ12_000543"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4988383.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(89544..91976)
                     /gene="ybiW"
                     /locus_tag="ACHJ12_000544"
     CDS             complement(89544..91976)
                     /gene="ybiW"
                     /locus_tag="ACHJ12_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO4988384.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(91982..92881)
                     /gene="ybiY"
                     /locus_tag="ACHJ12_000545"
     CDS             complement(91982..92881)
                     /gene="ybiY"
                     /locus_tag="ACHJ12_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4988385.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            93012..93674
                     /gene="fsa"
                     /locus_tag="ACHJ12_000546"
     CDS             93012..93674
                     /gene="fsa"
                     /locus_tag="ACHJ12_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4988386.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLATIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(93762..94511)
                     /gene="moeB"
                     /locus_tag="ACHJ12_000547"
     CDS             complement(93762..94511)
                     /gene="moeB"
                     /locus_tag="ACHJ12_000547"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4988387.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAAMIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(94511..95746)
                     /gene="moeA"
                     /locus_tag="ACHJ12_000548"
     CDS             complement(94511..95746)
                     /gene="moeA"
                     /locus_tag="ACHJ12_000548"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4988388.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDI
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVISKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
BASE COUNT        22912 a        24130 c        25495 g        23303 t
ORIGIN      
        1 agttaattaa atgttaatat cagcggggtg gatttatgcc tttattagta atcctgaaac
       61 tctgcgtcgt attagccagt gaccaaaaaa agaattaagg tcaaccgtgc tgtttttgct
      121 tcgtctcttt ttatctttaa ttgccaaccg aaactaattt cagccttata actcacacat
      181 tttaaacata aatgtcacta aagttacctt attgaaacat gattaacata atttgtagga
      241 attgatattt atcaatgtat aagtcttgga aatgggcatc aaaaagagat aaattgttct
      301 cgatcaaatt ggctgaaagg cggtaattta gctataaatt gatcaccgtc gaaaaatgca
      361 aatttgcttc aacaaaaacc tgtttattgt aaggattttg cggcgtaata tatacgtggg
      421 atcaatttga gtttttatta acatgtttgc aacctttctt tacgccgttt ttgtgtgcat
      481 tcacatggta tgatgaaagt gttcaaacaa atttctattg gggcatgcgt gtgacccttt
      541 ctaacggggt tcactctcgg agtcttcatg cgatgagcaa ggagtcatga tgttagatat
      601 agtcgaactg tcgcgcttac agtttgcctt gaccgcgatg taccacttcc tttttgtgcc
      661 actgacgctc ggtatggcgt tcctgctggc cattatggaa acggtctacg tcctctccgg
      721 caaacagatt tataaagata tgaccaagtt ctggggcaag ttgtttggta tcaacttcgc
      781 tctgggtgtg gctaccggtc tgaccatgga gttccagttc gggactaact ggtcttacta
      841 ttcccactat gtaggggata tcttcggtgc gccgctggca atcgaaggtc tgatggcctt
      901 cttcctcgaa tccacctttg taggtctgtt cttcttcggt tgggatcgtc tgggtaaagt
      961 tcagcatatg tgtgtcacct ggctggtggc gctcggttca aacctgtccg cactgtggat
     1021 tctggttgcg aacggctgga tgcaaaaccc aatcgcgtcc gatttcaact ttgaaactat
     1081 gcgtatggag atggtgagct tctccgagct ggtgcttaac ccggttgctc aggtgaaatt
     1141 cgttcacact gtagcgtctg gttatgtgac tggcgcgatg ttcatcctcg gtatcagcgc
     1201 atggtatatg ctgaaaggtc gtgacttcgc cttcgctaaa cgctcctttg ctatcgctgc
     1261 cagcttcggt atggctgctg ttctgtctgt tattgttctg ggtgatgaat ccggctacga
     1321 aatgggcgac gtgcagaaaa ccaaactggc tgctattgaa gccgagtggg aaacgcaacc
     1381 tgcgcctgct gcctttactc tgttcggcat tcctgatcag gaagaggaga cgaacaaatt
     1441 tgcgattcag atcccttacg cactgggcat cattgcaacg cgttccgtgg ataccccggt
     1501 tatcggcctg aaagagctga tggtgcagca tgaagaacgc attcgtaacg ggatgaaggc
     1561 gtactctctg ctcgaacaac tgcgttctgg ttctaccgac caggcggttc gtgaccagtt
     1621 caatagcatg aagaaagacc tcggttacgg tctgctgctg aaacgctata cgccaaacgt
     1681 ggctgatgcg actgaagcgc agattcaaca ggcaaccaaa gactccatcc cgcgtgtagc
     1741 gccgctgtac tttgcgttcc gtatcatggt ggcgtgtggc ttcctgcttc tggcaatcat
     1801 cgcgctctct ttctggagtg tcatccgcaa ccgcattggc gagaaaaaat ggcttctgcg
     1861 cgccgcgctg tacggtattc cgctgccgtg gattgctgta gaagcgggct ggttcgtggc
     1921 tgaatatggc cgccaaccgt gggctatcgg tgaagtgctg ccgacagctg tggcgaactc
     1981 gtcactgacc gcaggcgatc tcatcttctc aatggtgctg atttgcggcc tgtataccct
     2041 gttcctggtg gcagaattgt tcttaatgtt caaatttgca cgcctcggcc caagcagcct
     2101 gaaaaccggt cgctatcact ttgagcagtc ttccacgact actcagccgg cacgctaaga
     2161 caggagtcgt caaatgatcg attatgaagt attgcgtttt atctggtggc tgctggttgg
     2221 cgttctgctg attggttttg cagtcactga cggtttcgac atgggggtgg gcatgctcac
     2281 ccgtttcctc ggtcgtaacg acaccgagcg tcgaattatg attaactcca ttgcaccaca
     2341 ctgggacggt aaccaggttt ggctgatcac cgcgggcggc gcactctttg ctgcctggcc
     2401 gatggtctat gccgctgcgt tctccggctt ctatgtggcg atgatcctcg tgctggcgtc
     2461 tttgttcttc cgtccggtcg gttttgacta ccgctccaag attgaagaaa cccgctggcg
     2521 taacatgtgg gactggggca tcttcattgg tagcttcgtt ccgccgctgg taattggtgt
     2581 agcgttcggt aacctgttgc agggcgtacc gttcaacgtt gatgaatatc tgcgtctgta
     2641 ctacaccggt aacttcttcc agttgcttaa cccgttcggc ctgctggcag gcgtggtgag
     2701 cgtagggatg atcattactc agggcgcaac ctatctgcaa atgcgtaccg tgggcgaact
     2761 gcacctgcgt acccgtgcaa cggctcaggt ggctgcgctg gtgacactgg tctgtttcgc
     2821 actggctggc gtatgggtga tgtacggtat cgatggttat gtcgtgaaat cgacaatgga
     2881 ccattacgca gcctctaacc cactgaataa agaagtggtt cgtgaagctg gcgcatggct
     2941 ggttaacttc aacaacacgc caattctgtg ggctattccg gcgctgggtg tggttctgcc
     3001 gctgctgacc atcctgactg cacgtatgga taaagccgcg tgggcgtttg tgttctcctc
     3061 cctgacgctg gcctgcatca tcctgacagc cggtatcgca atgttcccgt ttgtgatgcc
     3121 gtccagcacc atgatgaacg caagtctgac aatgtgggat gcaacttcca gccagctgac
     3181 gcttaacgtc atgacctggg ttgcggtggt tctggtaccg atcattctgc tctacaccgc
     3241 ctggtgttac tggaaaatgt tcggtcgtat caccaaagaa gatattgaac gtaacaccca
     3301 ctctctgtac taagtaagga gctaaaaatg tggtatttcg catggattct gggaacgctt
     3361 cttgcctgtt cgtttggggt aatcaccgcg ctggcgcttg aacacgtcga atcaggcaaa
     3421 gccggtcaag aagacatctg atgagtaaga ttatcgcgac tttgtatgcg gtaatggaca
     3481 agcgccccct gcgggcgctt tccttcgtga tggcgcttct gttagcagga tgtatgtttt
     3541 gggacccatc tcgttttgcc gcgaagacca gtgaactgga aatctggcat ggtttattgc
     3601 tgatgtgggc cgtctgtgct ggtgtgattc acggcgtggg ctttcgtccg cagaaggttc
     3661 tttggcaagg gattttttgc ccattgcttg ccgatattgt tctcattgtc gggctgattt
     3721 tcttcttctt ttaaatcaga attctcttta aaaaattatg ggccgctcca ggcccataaa
     3781 tttttacgct cccttaactt gccctcattc ccaaacctca atcgcgcgcg tatagtagca
     3841 gcgtttaaaa gctctaactt ttgttgcatt accgggatgt aaagtgaata caacgctgtt
     3901 tcgatggccg gttcgcgtct actatgaaga taccgatgcc ggtggtgtgg tgtaccacgc
     3961 cagttacgtc gctttttatg aaagagcacg cacagagatg ctgcgtcatc atcacttcag
     4021 tcagcaggcg ctgatggctg aacgcgttgc ctttgtggta cgtaaaatga cggtggaata
     4081 ttacgcacct gcgcggctcg acgatatgct cgaaatacag actgaaataa catcaatgcg
     4141 tggcacctct ttggttttca cgcaacgtat tgtcaacgcc gagaatactt tgctgaatga
     4201 agcagaggtt ctggttgttt gcgttgaccc actcaaaatg aagcctcgtg cgcttcccaa
     4261 gtctattgtc gcggagttta agcagtgact gacatgaata tccttgattt gttcctgaag
     4321 gctagccttc tggttaaact tatcatgttg attttgattg gtttttcaat cgcatcttgg
     4381 gctattatta tccagcggac ccgtattctt aacgcagcgg cgcgcgaagc cgaagcgttt
     4441 gaagataaat tctggtctgg aatcgaactc tctcgcctct atcaagagag ccaggggaaa
     4501 cgggataatc tgactggttc ggaacaaatc ttttacagcg ggttcaaaga gtttgtgcgc
     4561 ctgcatcgtg ccaatagcca tgcgccggaa gccgtagtgg aaggggcgtc gcgtgctatg
     4621 cgtatctcca tgaaccgtga acttgaaaat ctggaaacgc acattccgtt cctcggtacg
     4681 gttggctcca tcagcccgta tattggtctg tttggtacgg tctgggggat catgcacgcc
     4741 tttatcgccc tcggggcggt aaaacaagca acactgcaaa tggttgcgcc cggtatcgca
     4801 gaagcgttga ttgcgactgc aattggtctg ttcgccgcaa ttccggcggt aatggcttat
     4861 aaccgcctca accagcgcgt aaacaaactg gaactgaatt acgacaactt tatggaagag
     4921 tttaccgcga ttctgcaccg ccaggcgttt accgttagcg agagcaacaa ggggtaagcc
     4981 atggccagag cgcgtggacg aggtcgtcgc gatctcaagt ccgaaatcaa cattgtaccg
     5041 ttgctggacg tactgctggt gctgttgctg atctttatgg cgacagcgcc catcatcacc
     5101 cagagcgtgg aggtcgatct gccagacgct actgaatcac aggcggtgag cagtaacgat
     5161 aatccgccag tgattgttga agtgtctggt attggtcagt acaccgtggt ggttgagaaa
     5221 gatcgcctgg agcgtttacc accagagcag gtggtggcgg aagtgtccag ccgtttcaag
     5281 gccaacccga aaacggtctt tctgatcggt ggcgcaaaag atgtgcctta cgatgaaata
     5341 attaaagcac tgaacttgtt acatagtgcg ggtgtgaaat cggttggttt aatgacgcag
     5401 cctatctaaa catctgcgtt tcccttgctt gaaagagagc gggtaacagg cgaacagttt
     5461 ttggaaaccg agagtgtcaa aggcaaccga acaaaacgac aagctcaagc gggcgataat
     5521 tatttcagca gtgctgcatg tcatcttatt tgcggcgctg atctggagtt cgttcgatga
     5581 gaatatagaa gcttcagccg gaggcggcgg tggttcgtcc atcgacgctg tcatggttga
     5641 ttcaggtgcg gtagttgagc agtacaaacg catgcaaagc caggaatcaa gcgcgaagcg
     5701 ttctgatgaa cagcgcaaga tgaaggaaca gcaggctgct gaagaactgc gtgagaaaca
     5761 agcggctgaa caggaacgcc tgaagcaact tgagaaagag cggttagcgg ctcaggagca
     5821 gaaaaagcag gctgaagaag ccgcaaaaca ggccgagtta aagcagaagc aagctgaaga
     5881 ggcggcagcg aaagcggcgg cagatgctaa agcgaaggcc gaagcagatg ctaaagctgc
     5941 ggaagaagca gcgaagaaag cggctgcaga cgcaaagaaa aaagcagaag cagaagccgc
     6001 caaagccgca gccgaagcgc agaaaaaagc cgaggcagcc gctgcggcac tgaagaagaa
     6061 agcggaagcg gcagaagcag ctgcagctga agcaagaaag aaagcggcaa ctgaagctgc
     6121 tgaaaaagcc aaagcagaag ctgagaagaa agcggctgct gaaaaggctg cagctgataa
     6181 gaaagcggca gcagagaaag ctgcagccga caaaaaagca gcagaaaaag cggctgctga
     6241 aaaggcagca gctgataaga aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc
     6301 agcggcaaaa gctgcagctg aaaaagccgc tgcagcaaaa gcggccgcag aggcagatga
     6361 tattttcggt gagctaagct ctggtaagaa tgcaccgaaa acggggggag gggcgaaagg
     6421 gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac aatggcgcat caggggccga
     6481 tatcaataac tatgccgggc agattaaatc tgctatcgaa agtaagttct atgacgcatc
     6541 gtcctatgca ggcaaaacct gtacgctgcg cataaaactg gcacccgatg gtatgttact
     6601 ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag gctgcgttgg cagcagctaa
     6661 acttgcgaag atcccgaaac caccaagcca ggcagtatat gaagtgttca aaaacgcgcc
     6721 attggacttc aaaccgtaat cgcgatgttg actgttcgga cggtcaacat caggcaccgg
     6781 ttgccacggg gttctggtag ttttgtgtat tttagtttgt taacattctg ctaaattatc
     6841 gtgggccatc ggtccagata agggagatat gatgaagcag gcattacgag tagcatttgg
     6901 ttttctcata ctgtgggcat cagttctgca tgctgaagtc cgcattgtga tcgacagcgg
     6961 tgtagattcc ggtcgtccta ttggtgttgt tcctttccag tgggcggggc ctggtgcggc
     7021 acctgaagat attggcggca tcgttgctgc tgacttgcgt aacagcggta aatttaatcc
     7081 gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg caggaagtac aaccagctgc
     7141 atggtccgca ctgggcattg acgctgtagt tgtcggtcag gtcactccga atccggatgg
     7201 ttcttacaat gttgcttatc aacttgttga cactggcggc gcaccgggta ctgtacttgc
     7261 tcagaactcg tacaaagtga acaagcagtg gctgcgttat gctggtcata ccgccagtga
     7321 tgaagtgttt gaaaaactga ccggcattaa aggtgcgttc cgtacccgta ttgcctacgt
     7381 tgttcagacc aacggcggtc agttcccgta tgaactgcgc gtatctgact atgacggtta
     7441 caaccagttt gtcgttcacc gttcaccgca gccgctgatg tctccggcgt ggtcaccaga
     7501 cggttctaaa ctggcttatg tgaccttcga aagcggtcgt tccgcgctgg ttattcagac
     7561 gctggcaaat ggcgctgtac gtcaggtggc ttcattcccg cgtcacaacg gtgcacctgc
     7621 attctcgcca gacggcagca aactggcatt cgccttgtcg aaaaccggta gtctgaacct
     7681 gtacgtaatg gatttggctt ctggtcagat ccgccaggtg actgatggtc gcagtaacaa
     7741 taccgaaccg acctggttcc cggatagcca gaacctggca tttacttctg accaggccgg
     7801 tcgtccgcag gtttataaag tgaatatcaa cggcggtgcg ccacaacgta ttacctggga
     7861 aggttcgcag aaccaggatg cggatgtcag cagcgacggt aaatttatgg taatggtcag
     7921 ctccaatggt gggcagcagc acattgccaa acaagatctg gcaacgggag gcgtacaagt
     7981 tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca cctaacggca ctatggtaat
     8041 ctacagctct tctcagggga tgggatccgt gctgaatttg gtttctacag atgggcgttt
     8101 caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc cctgcctggt cgccgtatct
     8161 gtgataataa ttaattgaat agtaaaggaa tcattgaaat gcaactgaac aaagtgctga
     8221 aagggctgat gattgctctg cctgttatgg caattgcggc atgttcttcc aacaagaacg
     8281 ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac tggtatggat gcgaacggcg
     8341 gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca aatgcaacag ctgcagcaga
     8401 acaacatcgt ttacttcgat ctggacaagt acgatatccg ttctgacttc gctcaaatgc
     8461 tggatgcaca tgcaaacttc ctgcgtagca acccgtctta caaagtcacc gtagaaggtc
     8521 acgcggacga acgtggtact ccggaataca acatctccct gggtgaacgt cgtgcgaacg
     8581 ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga ccagatctcc atcgtttctt
     8641 acggtaaaga aaaacctgca gtactgggtc atgacgaagc ggcatactcc aaaaaccgtc
     8701 gtgcggtact ggtttactaa gagaattgca tgagcagtaa cttcagacat caactattga
     8761 gtctgtcgtt actggttggt atagcggccc cctgggccgc ttttgctcag gcaccaatca
     8821 gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcactca acttgagcgt atttctaatg
     8881 ctcacagcca gcttttaacc caactccagc aacaactttc tgataatcaa tccgatattg
     8941 attccctgcg tggtcagatt caggaaaatc agtatcaact gaatcaggtc gtggagcggc
     9001 aaaagcagat cctgttgcag atcgacagcc tcagcagcgg tggtgcagcg gcgcaatcaa
     9061 ccagcagcga tcaaagcggt gcggcggcat caacgacgcc gacagctgat gctggtactg
     9121 cgaatgctgg cgcgccggtg aaaagcggcg atgcaaacac ggattacaat gcagctattg
     9181 cgctggtgca ggataaatcc cgccaggatg acgcaatggt ggcatttcag aatttcatca
     9241 aaaattaccc tgattcaact tacctgccaa acgccaatta ttggctgggt cagttaaact
     9301 acaacaaggg taaaaaagat gatgcggcgt actattttgc ttcggtagtg aaaaactatc
     9361 cgaagtcacc aaaggctgct gatgcgatgt ttaaagtcgg cgtcatcatg caggacaaag
     9421 gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat cagtaaatac cctggtactg
     9481 atggcgctaa acaggcacaa aaacgtctga acgcgatgta atgcataaca cacgaccaga
     9541 agtcgcatta tttctggtcg tgtcgtgcga atcataagca gttgagtgat ctgcatcgaa
     9601 atttttgttg cgctcaagtc tgaaatcagt aatatatgcc gccgttgcca cgggatatca
     9661 aacaaaccga aagcaacgaa aaagtgggtc gttagctcag ttggtagagc agttgacttt
     9721 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc actaacatag ttagttgtag
     9781 tatccagcgt agtattgggt gattagctca gctgggagag cacctccctt acaaggaggg
     9841 ggtcggcggt tcgatcccgt catcacccac caccgggtcg ttagctcagt tggtagagca
     9901 gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca gttttagcat
     9961 cgaagacaga tgttaagcgt gtaggataac gttgcgtcag caacggcccg tagggcgagc
    10021 gaagcgagtc atcctgcacg acccaccact aattacggtg ggttcggtgg aagtagtttg
    10081 tagtatccag cgcagtatcg ggtgattagc tcagctggga gagcacctcc cttacaagga
    10141 gggggtcggc ggttcgatcc cgtcatcacc caccactcgg gtcgttagct cagttggtag
    10201 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatttta
    10261 acatcaaact cagatgttaa gcgtgaagga taacgttgcg ccagcaacgg cccgtagggc
    10321 aagcgaagcg agtcatcctg cacgacacac caatcttaaa gattggcccc gagtagaaat
    10381 ctttcaggta acacccgtat gggtcgttag ctcagttggt agagcagttg acttttaatc
    10441 aattggtcgc aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg
    10501 cttttttgct atctgcgata ctcaaagatt cgaacctgca gcaggtttga gttgagcgca
    10561 gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg
    10621 cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctattc aggcatcctc
    10681 aatttcactt tgtaaacctg atgacatcgt cagagcttac tgtgcaagca actctacgtc
    10741 ggtggaatta ggcgtaattc gagtgacttt tccccaccat tcgactatct tgtttagcat
    10801 ataaaacaaa ttacaccgat aacagcgaat attacgctaa tgtcggtttt aacgttaagc
    10861 ctgtaaaacg agatggtaag atgagcgtaa tgtttgatcc agacacggcg atttatcctt
    10921 tccccccgaa gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa
    10981 aacgtctgct aaaagaacgt aatgcggtga tggttgccca ctactatacc gatcccgaaa
    11041 ttcaacaact ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct
    11101 tcggtgcaaa gcatcccgct tctactttgt tagtcgctgg ggtgagattt atgggagaaa
    11161 ccgccaaaat tctcagtccg gaaaaaacaa ttctgatgcc gacacttcag gctgaatgtt
    11221 cactggatct cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc
    11281 gtactgtcgt cgtctacgcc aacacttctg ctgcggtaaa agcgcgcgca gattgggtgg
    11341 taacttcaag cattgccgtc gaacttattg atcatcttga tagtttgggt gaaaaaatca
    11401 tctgggcacc cgacaaacat ctggggcgtt acgtgcaaaa acagacgggt ggagacattc
    11461 tatgctggca gggtgcctgt attgtgcatg atgaatttaa gactcaggcg ttaacccgct
    11521 tgcaagaaga atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg
    11581 tcgatatggc ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaaacattgc
    11641 cacatcagag gcttattgtg gcaaccgatc ggggcatttt ctacaaaatg cagcaggcag
    11701 tgccagataa agagttactg gaagcaccaa ccgcaggtga gggtgcaacc tgccgcagct
    11761 gcgcgcattg tccgtggatg gccatgaatg gccttcagtc catcgcagag gcattagaac
    11821 aggaaggaag caatcacgag gttcatgttg atgaaaggct gcgagagagg gcgctggtgc
    11881 cgctcaatcg tatgctggat tttgcggcta cactacgtgg ataacgaata ataaggcgta
    11941 acgttacgct ttgggggaaa gatggatttt tttagtgtgc agaatatcct ggtacatata
    12001 ccaatagggg caggcggtta tgatctctca tggatcgaag cggtaggcac gatcgccggg
    12061 ttgctgtgta ttggccttgc cagtctggag aagatcagca actacttctt tggcctgatc
    12121 aacgtcacct tgtttggcat tattttcttt cagattcagc tgtatgccag cctgctatta
    12181 caggtgtttt tctttgccgc gaatatttac ggttggtatg cgtggtcgcg acaaaccagt
    12241 cagaacgagg cggagttgaa aattcgctgg ttgccattgc cgaaggcact cagctggttg
    12301 gcggtttgcg ttgtttcgat tggtctgatg acggtattta tcaatccggt gtttgcattt
    12361 ttgacccgcg tggcagtcat gatcatgcaa gcattaggat tacaggttgt gatgcctgaa
    12421 ctgcaaccgg acgctttccc gttctgggat tcatgcatga tggtgttatc tatcgtggca
    12481 atgattctga tgacgcgtaa gtatgtggaa aactggctgt tgtgggtgat tattaacgtg
    12541 attagcgtcg ttatttttgc acttcagggc gtttacgcca tgtctctgga gtacatcatc
    12601 ctgaccttta ttgcgctcaa cggcagccgg atgtggatca acagcgcacg tgaaagaggc
    12661 tcacgcgcgc tgtcccatta atggtgatga tgtgaatggc cagacacccc ttcattcagg
    12721 tggcagtctg gcccatgaca aggttgatat tccatctgaa tggtggcgtg ctcaatctga
    12781 tagtgatcca tcaggtagtg ttggatctga tccaacaagg catcgtgatc gtgtggcggg
    12841 atcacctgca catgcaacgt catcaccggc ttctcgccta ccatccatac atgtacatgg
    12901 tgcacattgc ggacttccgg gatttcccgg cacatacgac gcttcagttc agcgatatcc
    12961 agcgataccg gtgcaccttc aagtaattca ttcacgctat ctttcaacag tcgccacgcg
    13021 ctacgcagaa ccagaagcga caccagtatc gagagaatgg ggtcagcagg tgtccagcca
    13081 gtccagataa taatcaacgc ggcaataata gccccaaccg aacccagcag gtccccgagc
    13141 acatgcagtg ccgctgctcg tacgttgagg tttttctctt cgctgccgtg atgaagtaac
    13201 cagaaagaaa gtatatttgc cagcaaccca gccacggcaa ttgccatcat catgccgccc
    13261 tcgaccggac gcggcgtgcg gaagcgttct atcgcctccc agacaatcaa aatggtaatt
    13321 accacgaggg ctatggcatt cacaaacgcc gcgagcgtgg tcagtcttag ccaaccgaaa
    13381 gtgtggcgaa tggtgggagg acgacgtgaa aattgcacgg cgagtagggc gaaaagcagg
    13441 gcggcagtat cggtcaacat atgacccgca tcggccagca atgccagaga accagaaaga
    13501 aaaccaccaa cgacttctac cagcataaac ccggcagtca cgccgaaagc atacaacaag
    13561 cgacgagcat tattatcttc tggcaggtgt gaggacgtgt gtgagtgtga gtgcgccatg
    13621 acgagtcatc ccttaattat tctcacttaa ctttatgaca tcatactgct tttagaagtg
    13681 aaaaattaaa agggagagac tccgctctcc cattattggc tattttgcag ggttactgcg
    13741 tggtaccgtc ggttttggta tcgacatcat tattgatgcc atcaccggtt tgtacctttt
    13801 tattgatatc cggacagcga ccatctttgc acatggtgtt cttgtgctct tcatctttgg
    13861 tcattccgtc attgttcatt gaagaaccat ccgaatgcag cattgtgcca ccagaaccgg
    13921 tatttacccc gttattgtcg acgttatttg gcgcgacatt ttcacgggcg tcaggggcta
    13981 cctggcccgc atcagctgcg gcgtttgcct ggccgttatt agtttgcgct ccgctatcgg
    14041 cagccagtgc ggcaccgctg gcaaggctta gagtggcagt cagaaataat gtggccagtt
    14101 ttgtcatttt cataggatgc tcctgttatg gtcgttatgt cggataacct cttccaacag
    14161 tgcatttgca ggtgaatata aggcattggt ttaagatttc agccaggtta tgaaacgcag
    14221 cagagaatct tgaaataatt aacaaacaaa ggagttacag ttagaaattg taggagagat
    14281 ctcgtttttc gcgacaatct ggcgtttttc ttgctaattc caggattaat ccgttcatag
    14341 tgtaaaaccc cgtttacaca ttctgacgga agatatagat tggaagtatt gcattcacta
    14401 agataagtat ggcaacactg gaacagacat gaattatcag aacgacgatt tacgcatcaa
    14461 agaaatcaaa gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa
    14521 tgccgcgaat acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga
    14581 tgatcgcctg ttggtggtga ttggcccatg ctcaattcat gatcctgtcg cggcaaaaga
    14641 gtatgccact cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat
    14701 gcgcgtctat tttgaaaagc cgcgtaccac ggtgggctgg aaagggctga ttaacgatcc
    14761 gcatatggat aatagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct
    14821 tgatattaac gacagcggtc tgccagcggc aggtgagttt ctcgatatga tcaccccaca
    14881 atatctcgct gacctgatga gctggggcgc aattggcgca cgtaccaccg aatcgcaggt
    14941 gcaccgcgaa ctggcatcag ggctttcttg tccggtcggc ttcaaaaatg gcaccgacgg
    15001 tacgattaaa gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc
    15061 cgtaacgaaa tgggggcatt cggcgattgt gaataccagc ggtaacggcg attgccatat
    15121 cattctgcgc ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga
    15181 agggctgaac aaagcaggcc tgccagcaca ggtgatgatc gatttcagcc atgctaactc
    15241 gtccaaacaa ttcaaaaagc agatggatgt ttgtgctgac gtttgccagc agattgccgg
    15301 tggcgaaaag gccattattg gcgtgatggt ggaaagccat ctggtggaag gcaatcagag
    15361 cctcgagagc ggggagccgc tggcctacgg taagagcatc accgatgcct gcatcggctg
    15421 ggaagatacc gatgctctgt tacgtcaact ggcgaatgca gtaaaagcgc gtcgcgggta
    15481 aggtttaatt gtcggatgcg ccgtcagagt ggcgtatccg atgaatcacc acaggcctga
    15541 taagtcgcgc agcgtcgcat caggcaatgt gctccattgt tagcaacaaa aaagccgact
    15601 cacttgcagt cggctttctc attttaaacg aatgacgttt acttcgcttt accctggttt
    15661 gcaaccgccg ctgctttcgc tgcgatctcg tcagcattac ccagataata gcgtttcagc
    15721 ggtttgaaat tctcgtcgaa ctcatacacc agcggcacgc cagtcgggat attaagctca
    15781 agaatctctt cttcgctcat gttatcaaga tatttcacca gcgcacgtaa agagttaccg
    15841 tgtgcagcga tgatcacgcg ctcaccgctc ttcatacgcg gcagaatggt ttcattccag
    15901 taagggatca cgcggtcaat ggtcagcgcc aggctttccg tcagcggcag ttctttttcg
    15961 ctcagtttcg cgtaacgcgg atcgtgaccc ggataacgct catcatcttt agtcagttcc
    16021 ggcggagtga ctgcaaaacc acgacgccac tgtttcacct gctcgtcgcc atacttttca
    16081 gcagtttccg ctttgttcag accctgcaac gcaccgtagt gacgttcgtt cagtttccag
    16141 gatttctcaa cgggcagcca tgcctgatcc agttcgtcca gcacattcca cagggtatgg
    16201 atagcgcgtt tcagcacaga ggtgtaagca aagtcaaagc tgtaaccttc ctctttcagc
    16261 agcttacctg ctgcttttgc ttcgcttacg cctttctcag acagatccac gtcgtaccaa
    16321 ccggtgaaac ggttttcttt gttccactga ctttcgccat gacgaaccag aaccagctta
    16381 gttacagcca tatacttact cctcaaatca ttttttaatg ataataattc tcattatatt
    16441 gccgtgacga agcaacagca atgcttacgc ataaccatag cgaaaatagt gtcgcagtgt
    16501 aaggttgttg tgaatattga gttgcaaata tgtcggtgtt tgctggtgat ttgagcaata
    16561 taaggataaa gccctcatta cgagggcttc aggtgctact cagcaataaa ctgatattcc
    16621 gtcaggctgg aatactcttc gccaggacgc aggaagcagt ccggttgcgg ccattcaggg
    16681 tggtttgggc tgtccggcag aaactcgctt tccagtgcca ggccttgcca gtcggcgtaa
    16741 ggttcagttc ctcttgatgg cgtgccgccg aggaagttgc cggagtagaa ttgcagagcc
    16801 ggagcggtgg tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc
    16861 actttcttgc catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg
    16921 cgctgatcgt cgtcggcaag aaactcactg gcgatgattt tggcgctacg gaaatcaaaa
    16981 gacgttccgg cgacagattt cagaccgtcg tgcggaatgc cgccttcatc aaccggcaga
    17041 tattcgtccg ccagaatctg caacttgtga ttgcgcacgt cagactgctc gccgtcaaga
    17101 ttgaaataga cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa
    17161 gtaatagaga tacggttatc gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc
    17221 gggaagccct gatcgccatc atctgaactc agggcaaaca gcacctgacg atcgttctgg
    17281 ttcacaatct gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg
    17341 ccctgacttg gcgaaagcgt cacggtttca ccgtcaaagg tataacggct attggcgatg
    17401 cggttggcat aacgaccaat agaggccccc agaaacgcag cctgatcctg atagcattcc
    17461 gggctggcac agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgggcg
    17521 gaaagtaaag tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa
    17581 gttaacagtc ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc
    17641 ctgctccttg tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat
    17701 attgttcagc gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga
    17761 tacagccgcc aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt
    17821 tgacgatttc taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag
    17881 aggcatgaga ttccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc
    17941 tggcagcttc aacggtgcgg gcgttttcag tcaggatatg acgcacgcgt ttcgccacga
    18001 ttgggtccag ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg
    18061 gctgctggaa gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc
    18121 caaccagggt acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg
    18181 aaactgcttt agtccccagt gagcggcagt caatcagcaa ggcatgatct ttcttgccga
    18241 gtgcggaaat tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt
    18301 cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata
    18361 cggttccgac cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc tgcggcacat
    18421 tgccgctgat caccatgtcc acgccgccga agctgttgtt acgcagttgc agatgtttca
    18481 ccacgccacg aacgtagtta gcccattgat agttttcatg tgcgacaatg ggcgcatcga
    18541 gggaaaactc gtcgagctga ttttcataat cggctgccat cacgcgaact ttacggtcat
    18601 cgcgtggtgc acaactgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt
    18661 cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag
    18721 tggcagggta gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt
    18781 cttacactcc ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct
    18841 gttctgcggt caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac
    18901 gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat
    18961 tctcttcgcc attaaatggt gcgccgtgcc agcccataga gtaggggaag gaacactgga
    19021 agaggttgtc ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg
    19081 cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg
    19141 gccaggcagc ccagtaaggc acaacggcta accagtgttc ggtttcgaca acggtacggc
    19201 taccgtctgc cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa
    19261 aatattcttt ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc
    19321 aaatctgtcc gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga
    19381 cctgcaccca cgggtacgtt tttcccagct ctgcggtttg ctcctgccag gttttgacga
    19441 tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc ggtgaaaagc
    19501 agatcacccg gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg
    19561 gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcctgg
    19621 tgtaatcggg gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg
    19681 gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct
    19741 tagcgcggtg cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg
    19801 gatgatcaac gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat
    19861 ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc
    19921 agttcagttc acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc
    19981 gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag gcattaacca
    20041 cgtccagcac gctgttgcct acgccagcgc cgaggttgta gatgtgtacg cctggcttgt
    20101 tcgccagttt ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat
    20161 cgcgtacgcc agtaccatct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc
    20221 gacggcctac agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat
    20281 cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcaa
    20341 tgctccagtc cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc
    20401 ttttgccgta agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg
    20461 gctgatcgcc ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc
    20521 gcatggcgct aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat tccagcggtt
    20581 tttgtaccga ttcgccgacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt
    20641 gatcgtgcag tatctcggtc atcaacgctt cgttacgaat atcgccttca acaaacgttg
    20701 gatgtttgcc gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt
    20761 tatcaagaat gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa
    20821 tgtaaccgct accaccggta accagaactc tcataattcg ctccattagg cttatggtat
    20881 gaaataacca tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc
    20941 gtttacacaa gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggtt
    21001 tatgaatgat tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc atgcgtcgaa
    21061 ccgttggccg gagagggcgc ttaggccgcc tccggcaagg tcagcactac cgactcaata
    21121 tatttttgtc agcacatagc gatagagtcc accgtccggc acgaactcaa ggcgatgggt
    21181 aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc
    21241 gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat caagcccctg
    21301 tagtggttca tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga cgatcagcgc
    21361 cagacgctgc tgtccccagg aaagactatg gaacggagcg tcagccgtac gtttatcaat
    21421 gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa cggcctgata
    21481 aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacggtag tgctgacccg
    21541 gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat cccagatggt
    21601 ttcgccgctg ccgcgacgtc ggccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg
    21661 atcgccagta atcaggctta ataacgtcga ttttcccgca ccatttggcc cgacaatttg
    21721 ccagtgctcg ccaggattca cctgccagct aaggttatta agaatggggc gatcgttata
    21781 agaaaccacg ccattattca gcacaatgcg cggttcgttg gtgggtaagg cgtgacgtgc
    21841 tgaaggttca tccggctccg gcagttgcac accttcaagc tgttcgctat gcgccagttg
    21901 cgcgacgagt gcttgttgga gcagttcctc tttagcgcca gtttccgcta acgtacaatc
    21961 cgccagcacg ccagcaaact ggacaaattc cgggatctca tcgaagcgat tgagcaccag
    22021 taccagagta ataccggact gatgtaacga ggcgagtcgc tcagccagct gctgacgtga
    22081 ggcaacatcc aggccatcga acggctcatc aagaatcaac aagtcaggct ccgacatcag
    22141 cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc
    22201 gaggagggcg gtaataccga actgctgcgc cagttgcatg caacgcggtg catccttaac
    22261 ttcatcctga atgatctcag ccgtagtgcg tccggtgtca tcttcgccag ggccgagcat
    22321 atcggtgtta ttccgctgcc attcgtcgct gacgagcttt tgcaattgct cgaaggagag
    22381 acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa gttcccccgc
    22441 cagcgcacgg gccagcgccg actttccact tccattcgaa ccgacaaacg cccaactatc
    22501 acccgcgttt aacgttagct gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgt
    22561 gccttgcaaa atttgcaacg atgacatttt atatcccatt ttgtgcagcg attactgata
    22621 gggatacgtg tttcataaca aattgtcaac acgcttagca tagcgtggcg ataatcacgc
    22681 tgtcggcatt aaagtaggcc gtgacattct gtccttgctg aagagaagtc gcttcattaa
    22741 ccggcactgt ggcgcacagt gtttgcccgt cgggtagcgc cattaatact tcgcactgct
    22801 ctgcgccgcg ctcaatatga ctaataatac ccggtaattg gttgtcggcg ttttgtgcga
    22861 ccgcttcgtc ctgagtaatg cctacccacg gcgcttttag caatatcaac acttctttgc
    22921 cttcatccag ccccagacgt gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt
    22981 ttccgtcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg cgggcggtga
    23041 tggtgccgaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca
    23101 gcaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct ttttgctgga
    23161 tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc agtactgcgc
    23221 cgccgccacc tttaccgcct gttgcgcgct cgaccagaat atgctcactt aactgattca
    23281 tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc gctccctggc
    23341 taatggaacc ggaaagcgca atgtgtttta gtagcgaaat ccggcgcggg tcggcgaata
    23401 atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg tcctggcaaa
    23461 agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct
    23521 tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac catgttagag
    23581 ttattaaaaa gtctggtatt cgccgtaatc atggtacctg tcgtgatggc catcatcctg
    23641 ggtctgattt acggtcttgg tgaagtattc aacatctttt ctggtgttgg taaaaaagac
    23701 cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt ttctttttct
    23761 caacttcctg cttttcctgc cgatattttt tcttatctac ctcacaaagg ttagcaataa
    23821 ctgctgggaa aattccgagt tagtcgttat attgtcgcct acataacgtt acattaaggg
    23881 gttaccaatg gctcgtaaat ggttgaactt gtttgccggg gcggcactct ctttcgctgt
    23941 tgctggcaat gcactggcag atgaagggaa aatcacggtg ttcgccgccg catcactgac
    24001 taacgcaatg caggacattg ctacgcagta taaaaaagag aaaggcgtgg atgtggtttc
    24061 ttctttcgct tcgtcatcta ctctcgcccg tcagattgaa gcgggtgcgc ctgcggatct
    24121 gtttatttct gccgatcaga aatggatgga ttatgcggtt gataaaaaag cgatcgatac
    24181 ggcttcgcgc cagacactgc tcggcaatag cctggtcgtt gtagcaccga aagccagcga
    24241 gcagaaagat ttcaccatcg acagcaaaac caactggact tcgctgctga atggcggtcg
    24301 tctggcggtt ggcgatccgg aacatgttcc cgctggcatt tatgcaaaag aagcactgca
    24361 aaaactgggc gcatgggata cgctctctcc gaaactggct ccggcggaag atgttcgtgg
    24421 cgcgctggcg ttggtcgaac gcaatgaagc gccgctgggc atagtttacg gttctgacgc
    24481 agttgccagc aaaggggtaa aagtggtcgc caccttcccg gaagattcgc ataaaaaagt
    24541 ggaatacccg gttgctgttg tggaagggca taacaatgcg acagtgaaag cattttatga
    24601 ttatttgaaa gggccgcagg ctgcggaaat ctttaaacgt tacggattta caaccaagta
    24661 atgatactga ccgatccaga atggcaggca gttttattaa gcctgaaagt ttcttccctg
    24721 gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct ggttactggt gcgttgcacg
    24781 tttccgggca aagctctgct cgacagcgta ctgcatctac cgctggtgtt accgcccgtg
    24841 gtcgtcggtt acttattatt agtttcgatg ggacggcgcg ggtttatcgg tgaacgtctg
    24901 tatgactggt ttggcattac cttcgccttt agctggcgcg gcgcggttct cgctgccgcc
    24961 gtcatgtcgt ttccgctgat ggtgcgggca attcgtctgg cgctggaagg ggttgatgtc
    25021 aaactggaac aggccgcaag aacactgggg gccgggcgct ggcgcgtttt ctttactatc
    25081 acgataccgc tgaccttacc gggaattatt gttggtacgg tactggcttt tgctcgttct
    25141 ctcggtgaat ttggcgcaac catcaccttt gtgtcgaaca ttcctggtga aacgcgaacc
    25201 attccttctg ccatgtatac cctgattcag acccccggcg gggaaagtgg cgcggcgaga
    25261 ctgtgcatta tttctattgc gctggcgatg atctccctgt tgatttcaga atggctggcc
    25321 agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga actgaatttt tcccagacct
    25381 tgggcaacca ttgcctgacc attaatgaaa cgctgcccgc caatggcatc actgctatct
    25441 ttggcgtctc cggtgccgga aaaacttcgc tgattaacgc catcagtgga ctgacgcgcc
    25501 cgcaaaaagg gaggattgtc ctcaatggtc gggtactaaa tgatgccgaa aaaggtatct
    25561 gcctgacgcc agaaaagcgt cgcgttggct atgtttttca ggatgcgcgg ctgtttccgc
    25621 attacaaagt gcgtggcaat ctgcgctacg gcatggcgaa aagcatggtc gatcagttcg
    25681 ataagctggt ggcgctttta ggcattgaac cgttgcttga ccgtttacca ggcagcctgt
    25741 ccggaggcga aaaacagcgc gtagcgattg ggcgggcttt gctgacggca ccagaattgc
    25801 tgttgctgga tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa ctgttgcctt
    25861 atctgcaacg tctgacgcgg gaaatcaaca tcccgatgtt gtatgtcagc cattcgctgg
    25921 atgagatcct ccatctggca gacagagtga tggtactgga aaacggtcag gtgaaagcct
    25981 ttggcgcact ggaagaagtc tggggtagta gcgtgatgaa tccgtggctg ccgaaagagc
    26041 aacaaagtag cattctgaaa gtgacggtac ttgagcatca tccgcattac gcgatgactg
    26101 cgctggcgct gggcgatcag catttgtggg tcaataagct ggacgaaccg ctgcaagctg
    26161 cgctacgtat ccgcattcag gcttccgatg tttctctggt tttacaaccg ccgcagcaaa
    26221 ccagcattcg taacgtattg cgggcaaaag ttgttaatag ttatgacgac aacggccagg
    26281 tggaagtgga actggaagtc ggaggtaaaa cgctgtgggc gcgtatcagc ccgtgggcca
    26341 gggatgaact ggtgatcaaa cctggcctgt ggctgtacgc gcaaattaaa agtgtgtcga
    26401 taaccgcctg attaaatcag gtggctataa atgaactggg caatgctgtc ggtggtgtta
    26461 tcaccaatca caatgttggc gcgcgctttt accgcgtcat cggcgttgcc catcgccacg
    26521 cctgtaccag cggcctccag catactgata tcattaaagt tatcgccgaa cgccacgacg
    26581 ttttccatcg accagccttg cgcctcaacc catttcgtca aacgtttacc tttgctgttg
    26641 ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt cacactccag tcccagttca
    26701 tgttcgacat gcttaccaaa atgctgcaat tgcggcaggt catcgtgcgt cagggcgaac
    26761 ttccatacgg cgttaacttg ttgcgccgtt tcagccagag aagcgacttg tgtgaaagtc
    26821 ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac atgcccggtc
    26881 gggtgctcat agaccattgc atcatccaca tacatcagac cgtgaatgtg gtgttcattc
    26941 agcatctcaa tgagttgcag ggctttatta acgggcattg ggtccgcttc cagcacggtt
    27001 tttgcatgat aatcatacaa ataggtgcca ttacagcaaa tagcaggtgt atccagcgcc
    27061 agcgcctgat aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat gattaattga
    27121 tagcctgctt cgcgagcgcg ggccagggct tctatcgatg aaggaagcag ggtctttttc
    27181 ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat ggcgtattcc
    27241 agattaaggt taagaatttt cgtctgcgcg aatggtacac cgataccact atcggacaaa
    27301 attctgcgtt ttaattcagc attcaccgcc aaaagcgact aattttagct gttacagtca
    27361 gttgctaaat gcaaaggagc attcatgaag caaacagttt atatcgccag ccctgagagc
    27421 cagcaaattc acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc
    27481 gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt
    27541 ggtgttcgcc ctgagtttcg cgtcctggcg tatagtatcg ccccggacga tggcgcactg
    27601 acctttgccg cagagtctgc gctgccgggt agtccgacgc atatttccac cgatcaccag
    27661 gggcagtttg tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa
    27721 gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg ccattccgcc
    27781 aatatctcac cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc
    27841 ctgtttacgg tcagcgatga tggtcatctc gtggcgcagg accctgcgga agtgaccacc
    27901 gttgaagggg ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc
    27961 gtcaatgagt taaacagctc attggatgtc tgggaactga aagatccgca cggtaatatc
    28021 gaatgtgtcc agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct
    28081 gatattcata tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagcctg
    28141 attaccgttt tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca
    28201 acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct gattgccgcc
    28261 gggcaaaaat ctcaccacat ctcggtatac gaaattgttg gcgagcaggg actactgcat
    28321 gaaaaaggcc gttatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa
    28381 ccgctgattt aaccggcgca gtctctcctg cgccggtgta ttaacctatc tcctgtaacg
    28441 catgtctctg gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat
    28501 taatcagcaa ggcaaaaaag cgttctgctg ccggagaaag cacggcatct ttgcggcgaa
    28561 taatccccaa tgtccgacgt ataacgggtt ccaccagtgg aataccaata atcgaagaat
    28621 agggcgcatg gggcattgcc agaccaggaa gcgccgaaat ccccagtccc gcctctacca
    28681 gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt
    28741 cggctagctg ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt
    28801 tgtaacccac taactcctgc cattctacaa gctgcttttt ggccaatgga tggtcacgcc
    28861 gacaggccag cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga
    28921 cgttgttcat gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat
    28981 tcgtgccttg ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga
    29041 ttgccagcgg taaaaaataa aatactgcgg tcggaataca cgctaacgtc accataccac
    29101 gatgataagc attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt
    29161 cccgcgcctc cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac
    29221 gctcaaatag ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag
    29281 gttgcgtaat attgagtaat ttagcagcat tgttaaatga actggactct gccagtatga
    29341 caaatgcctt catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg
    29401 attttgggtg gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa
    29461 aaatgttatt aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata
    29521 tcgcaataag ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat
    29581 gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg gaacatttac ccgaagatca
    29641 aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga
    29701 cggtattggc ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga
    29761 tccgcgtgct gatgttgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga
    29821 taccacgcct aactgcggca atatgctttc tggcgttggg gcattcgcca ttgaaaatgg
    29881 tttgattgca gggacatcac cggtcacccg tgtgcgtatc cgcaacgtca atacgggtac
    29941 gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagtgccag
    30001 aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg
    30061 aaccaaaacc ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc
    30121 ggtgacctgt atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa
    30181 aacaggttat gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga
    30241 atctatccgt ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat
    30301 ccctaaacct gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt
    30361 tatgccgcat tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag
    30421 ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct tctgtaggat acggcaatat
    30481 caatattgaa caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc
    30541 cacgacgtta cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta
    30601 tcttccctga aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag
    30661 tcatacagaa agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat
    30721 attagcgatc ccatgtatta ttggctttat gccagctccg gcaggattaa gcgaactggc
    30781 ctgggtgctt tttggtattt acctggcggc cattgtgggg ctggttatca agcctttccc
    30841 ggaacctgtc gtactgttaa ttgccgttgc tgcctcgatg gtggtggtcg gtaacttatc
    30901 cgacggtgcg tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct
    30961 ggtgttctcg gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacgtat
    31021 tgcctatctg ctgattggta aaatcggtaa caccacgctg ggtctgggtt acgttacggt
    31081 attcctcgat ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat
    31141 tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc
    31201 gcgtcgtgtc ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta
    31261 tatgttcttt accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct
    31321 gcacctgcaa attagctggg gtggatgggc actggccgcc ggattgccgg gcatcattat
    31381 gctgctggtc accccactgg tgatttatac catgtatccg ccagaaatta agaaggtgga
    31441 taataaaacc atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa
    31501 aatgctgctc ggtgtctttg tgctggcgct gctgggctgg attttcagta agtctctggg
    31561 ggttgatgaa tccaccgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt
    31621 tacctgggaa gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg
    31681 tattatcgga ttaagctcct tattatcgaa agttaaattc ttcgaatggt tagctgaagt
    31741 ctttaaaaat aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt
    31801 cctcagcatt atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt
    31861 accggtattt gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc
    31921 actgttgttc tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc
    31981 ggtcatcttt ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact
    32041 gacgatatta accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat
    32101 cggctggaac atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtttgcg
    32161 ttcgctcacc ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt
    32221 cctgcaactc gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg
    32281 cgcagatatc tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt
    32341 aataacgaaa taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagccaaa
    32401 aaaggcacta ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa
    32461 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
    32521 accgctaaag cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
    32581 aactcactct gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg
    32641 gcggcccagc gttatggcgg tatttttgtg cctccgcaca ttgcggtcat ccatcaatat
    32701 atgcgtgaga tgatggcagg cggcggcaaa atgatcctcg ggtcagacag ccacacccgt
    32761 tacggtgcat tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg
    32821 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
    32881 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
    32941 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
    33001 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
    33061 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
    33121 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
    33181 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca
    33241 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
    33301 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
    33361 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
    33421 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
    33481 cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggg
    33541 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
    33601 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
    33661 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
    33721 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
    33781 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
    33841 actcagcaac cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg
    33901 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
    33961 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
    34021 tttaccctgt cacgccgcga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
    34081 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
    34141 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtatat
    34201 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
    34261 ggcggtctgg cgaatattgc cgaagagtac gcgactaaac gctaccgttc taacgtcatc
    34321 aactggggga tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac
    34381 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
    34441 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct
    34501 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
    34561 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
    34621 gcatcttatc cgacctacga atcgtatcga atctgtaggc cagataaggc attttcgcag
    34681 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
    34741 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
    34801 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
    34861 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
    34921 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
    34981 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
    35041 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
    35101 aggaaaccgt agtaaatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
    35161 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
    35221 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacggttg
    35281 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
    35341 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
    35401 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
    35461 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
    35521 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
    35581 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
    35641 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
    35701 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
    35761 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
    35821 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
    35881 gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
    35941 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
    36001 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
    36061 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
    36121 aagcctgctt ttttatacta acttgagcga aacgggaagg taaaaagaca aaaagttgtt
    36181 tttaatacct ttaagtgata ccagatggca ttgcgccatc tggcagagtg attaactaaa
    36241 catcgcagta atcgaggcgc ttgccagaga gtggaaatga acgttaaacc cgaccatcgc
    36301 gccgctggca ccttcatcga catcaatacg ttctatatcc agcgcgtgaa cggtaaaaat
    36361 gtagcgatga gtttcgcctt tcggcggtgc tgcgccatcg tacccggttt taccaaagtc
    36421 ggtacgcgtc tgcaaaacgc cgtctggcat tgctaccaga ccagagccaa acccttgcgg
    36481 taatacgcgg gtatcagcgg gtaagttaac aactacccag tgccaccagc cggagccggt
    36541 tggcgcatcc gggtcgtagc aggtgacaac aaaacttttc gttcccgcag gaacatcatc
    36601 ccacgccaga tgcggtgaaa tattatcgcc atcgtaaccc atgccgttaa agacatgacg
    36661 atgcggcaat ttatcgccat cgcgcagatc gttactgatg agtttcatga accctccttt
    36721 cttgtttgca gaaagtgtag ccagaaaccc tcacgcggac ttctcgttat tggcaaaaaa
    36781 atgtttcatc ctgtaccgcg cggttaaccg ctgcggtcag acgctgcaac tgttgcggga
    36841 gaataatata gggcggcatc aggtaaatca gtttgccaaa aggccggatc cagacaccct
    36901 gttcgacaaa gaatttttgc agcgccgcca tattcaccgg atgagtggtt tcgactacgc
    36961 cgattgcccc cagcacgcgc acatcggcaa ccatttcggc atcacgggcg ggggcaagtt
    37021 gctcgcgcag ctgtacttca atagccgcca cctgttgctg ccagtcgcca gattcgagaa
    37081 tcgccaggct ggcgtttgct gccgcgcagg ccagcggatt gcccataaaa gttggcccat
    37141 gcataaagca accggcttcg ccgttactga tggtttccgc aacctcgcgc gtggtgagcg
    37201 tggcggaaag ggtcattgtg ccgccggtta aggctttacc gaggcacaaa atgtccggcg
    37261 cgatttctgc atgttcacag gcaaacagtt tgccggtacg accaaatccg gtggcgatct
    37321 cgtcggcaat cagcaagata ccttcgcgat cgcacatttt gcggattcgt tttaaccatt
    37381 ccggatggta catgcgcatc ccgcctgcgc cctggacaat cggttcaatg atcaccgccg
    37441 cgatttcatg acgatgcgcc gccatcaggc gggcaaagcc aaccatatca cgttcatccc
    37501 attcaccatc catgcggctt tgcggggcgg gagcaaacaa gttttctggc agatagcctt
    37561 tccacaggct gtgcattgag ttgtccggat cgcacaccga catcgcgcca aaggtatcgc
    37621 catgataacc attgcggaag gtcagaaaac gctggcgcgc ttcgcctttg gcctgccagt
    37681 actgcaacgc cattttcatc gccacttcca ccgctacgga gccggagtcc gcgagaaaaa
    37741 cgcattccag cggttgcggc gtcattgcca ccagtttgcg gcatagctcg atggcgggcg
    37801 catgggtgat accgccaaac atcacatgcg acatagcatc aatttgcgac ttcatcgccg
    37861 cattaagctg cgggtgatta tagccgtgga tcgccgccca ccaggacgac ataccgtcaa
    37921 ccaggcgtct gccgtcagac aaaattagct cgcaaccttc ggcgctcgcg accggataaa
    37981 ccggcagagg ggaggtcatg gatgtgtatg ggtgccagat atggcgttgg tcaaaggcaa
    38041 gatcgtccgt tgtcataatc gacttgtaaa ccaaattgaa aagatttagg tttacaagtc
    38101 tacaccgaat taacaacaaa aaacacgttt tggagaagcc ccatggctca ccgcccacgc
    38161 tggacattgt cgcaagtcac agaattattt gaaaaaccgt tgctggatct gctgtttgaa
    38221 gcgcagcagg tgcatcgaca gcatttcgat cctcgtcagg tgcaggtcag cactttgctg
    38281 tcgattaaga ccggagcttg tccggaagat tgcaaatact gcccgcaaag ctcgcgctac
    38341 aaaaccgggc tggaagccga gcggttgatg gaagttgaac aggtgctgga gtcggcgcgc
    38401 aaagcgaaag ctgcgggatc gacgcgtttc tgtatgggcg cggcgtggaa gaatccccac
    38461 gaacgcgata tgccgtacct ggaacaaatg gtgcaggggg taaaagcgat ggggctggag
    38521 gcgtgtatga cgctgggcac gttgagtgaa tctcaggcgc agcgcctcgc gaacgccggg
    38581 ctggattact acaaccacaa cctggacacc tcgccggagt tttacggcaa tatcatcacc
    38641 acccgcactt atcaggaacg cctcgatacg ctggaaaaag tgcgcgatgc cgggatcaaa
    38701 gtctgttctg gcggcattgt gggcttaggc gaaacggtaa aagatcgcgc cggattattg
    38761 ctacaactgg caaacctgcc gacgccgccg gaaagcgtac caatcaacat gctggtgaag
    38821 gtgaaaggca cgccgctggc ggataacgat gatgtcgatg cctttgattt tattcgcacc
    38881 attgcggtcg cgcggatcat gatgccgacc tcttacgtgc gcctttctgc cggacgcgag
    38941 cagatgaacg aacagactca ggcgatgtgc tttatggcag gcgcaaactc gattttctac
    39001 ggttgcaaac tgctaaccac gccgaatccg gaagaagata aagacctgca actgtttcgc
    39061 aaactggggc taaatccgca gcaaactgcc gtgctggcag gggataacga acaacagcag
    39121 cgtctggagc aggcactgat gaccccggac actgacgaat attacaacgc ggcagcacta
    39181 tgagctggca ggagaaaatc aacgcggcgc tcgatgcgcg gcgtgctgcc gatgccctgc
    39241 gtcgccgtta tccagtggcg caaggagccg gacgctggct ggtggcggat gatcgccagt
    39301 atctgaactt ttccagtaac gattatttag gtttaagcca tcatccgcaa attatccgtg
    39361 cctggcagca gggtgcggag caatttggcg tcggtagcgg cggttccggt cacgtcagcg
    39421 gttatagcgt tgcgcatcag gcgctggaag aagaactggc cgagtggctg ggctattcgc
    39481 gggcactgct atttatctct ggttttgccg ctaatcaggc ggtcattgcc gcgatgatgg
    39541 caaaagagga ccgcattgtc gctgaccggc ttagccatgc ctcattgctg gaggctgcca
    39601 gtttaagccc gtcgcagctt cgccgttttg ttcataacga tgtcactcat ctggcgcgac
    39661 tgcttgcttc cccctgtccg gggcagcaac tggtggtgac agaaggcgtg ttcagcatgg
    39721 acggcgatat tgcgccactg gcggaaatcc agcaggtaac gcaacagcac aatggctggt
    39781 tgatggtcga tgatgcccac ggcacgggcg ttatcgggga gcaggggcgc ggcagctgct
    39841 ggctgcaaaa ggtaaaacca gaattgctgg tggtgacttt tggcaaagga tttggcgtca
    39901 gtggggctgc ggtgctttgc tccagtacgg tggcggatta tctgctgcaa ttcgcccgcc
    39961 accttatcta cagcaccagt atgccgcccg ctcaggcgca ggcattacgt gcgtcactgg
    40021 cggtcattcg ccgtgatgag ggggatgcac ggcgcgaaaa actggtgtca ctcattgcgc
    40081 gttttcgtgc cggagtacag gatttgccgt ttacgctggc tgattcatgc agcgccatcc
    40141 agccattgat cgtcggtgat aacagccgtg cgttacaact ggcagaaaaa ctgcgccagc
    40201 aaggctgctg ggtcacggcg attcgcccgc caaccgtacc cgctggtact gcgcgactgc
    40261 gcttaacgct aaccgcagcg catgaaatgc aggatatcga ccgtctgctg gaggtgctgc
    40321 atggcaacgg ttaataaaca agccattgca gcggcatttg gtcgggcagc cgcactctat
    40381 gagcaacacg cagatctaca gcgccagagt gctgacgtct tactggcaat gctcccacag
    40441 cgtaaataca cccacgtact ggacgcgggt tgtggacctg gctggatgag ccgccactgg
    40501 cgggaacgtc acgcgcaggt gacggcctta gatctctcgc cgccaatgct tgttcaggca
    40561 cgccagaagg atgccgcaga ccattatctg gcgggagata tcgaatccct gccgttagcg
    40621 actgcgacgt tcgatcttgc atggagcaat ctcgcagtgc agtggtgcgg taatttatcc
    40681 acggcactcc gcgagctgta tcgggtggtg cgccccggag gcgtggtggc gtttaccacg
    40741 ctggtgcagg gatcgttacc cgaactgcat caggcgtggc aggcggtgga cgagcgtccg
    40801 catgctaatc gctttttacc gccagatgaa atcgaacagt cgctgaacgg cgtgcattat
    40861 caacatcata ttcagcccat cacactatgg tttgatgatg cgctcagtgc catgcgttcg
    40921 ctgaaaggca tcggtgctac gcatcttcat gaagggcgcg acccgcgaat attaacgcgt
    40981 tcgcaattgc agcgattgca actggcctgg ccgcaacaac aggggcgata tcctctgacg
    41041 tatcatcttt ttttaggagt gattgctcgt gagtaaacgt tattttgtca ccggaacgga
    41101 taccgaagtg gggaaaaccg tcgccagttg tgcactttta caagccgcga aggcagtagg
    41161 ttatcggacg gcaggttata aaccggtcgc ctctggcagc gaaaagaccc cggaaggctt
    41221 acgtaatagt gacgcgctgg cgttacagca caacagcagc ctgcagctgg attacgcaac
    41281 ggtaaatcct tacaccttcg cagaacccac ctcgccgcac atcatcagcg cgcaagaggg
    41341 caggccgata gaatcattgg taatgagcgc cggattacgc gcgcttgaac aacaggctga
    41401 ctgggtgtta gtggaaggtg ctggcggctg gtttacgccg ctttctgaca ctttcacttt
    41461 tgcagattgg gtaacacagg aacaactgcc ggtgatactg gtagttggtg tgaaactcgg
    41521 ctgtattaat cacgcgatgt tgactgcgca ggcaatacaa cacgccggac tgactctggc
    41581 gggttgggtg gcgaacgatg ttacgcctcc gggaaaacgt cacgctgaat atatgaccac
    41641 gctcacccgc atgattcccg cgccgctgct gggagagatc ccctggcttg cagaaaatcc
    41701 agaaaatgcg gcaaccggaa agtacataaa ccttgccttg ttgtagctat cctgtatttg
    41761 gttaaattgc gagcgagatc gcgtcttcga ttgactgcaa tttaaccaat taaattctaa
    41821 aataatcacg aaaaaaattt tacttccgcc tcatgcggcg aatgtgggaa ttgcccaggc
    41881 ggcgggggat aggggctgga gacagttatc cactattcct gtggataacc atgtgtatta
    41941 gagttagaaa acacgaggca agcgagagaa tacgcggctt gcacgcgaat tggcgttaaa
    42001 gacggctcaa agaaatatct tttatttttt aactggttag ataaatgcaa tggcagtcac
    42061 tgaacaggca tctcttgcca taaaactgtc atcactcatc ttgacaaatg ttaaaaaagc
    42121 cgttgctttg gggataaccc ggtaaggccg gagttttatc tcgccacaga gtaaattttg
    42181 ctcatgattg acagcggagt ttacgctgta acagaaatat tatggtgatg aactgttttt
    42241 ttatccagta taatttgttg gcataattaa gtacgacgag taaaattaca tacctgcccg
    42301 cccaactcct tcaggtagcg actcatgagt aaaccgttca aactgaattc cgcttttaaa
    42361 ccttctggcg atcagccaga ggcgattcga cgtctcgaag aggggctgga agatggcctg
    42421 gcgcaccaga cgttacttgg cgtgactggc tccgggaaaa cattcaccat tgccaatgtc
    42481 attgctgacc ttcagcgccc aaccatggta cttgcgccca acaaaacgct ggcggcccag
    42541 ctgtatggcg aaatgaaaga gttcttcccg gaaaacgcgg tggaatattt cgtttcctac
    42601 tacgactact atcagccgga agcctatgta ccgagttccg acactttcat tgagaaagat
    42661 gcctcggtta acgaacatat tgagcagatg cgtttgtccg ccaccaaagc gatgctggag
    42721 cggcgtgatg tggttgtggt ggcgtctgtt tccgcgattt atggtctggg cgatcctgat
    42781 ttatatctca agatgatgct ccatctcacg gtcggtatga ttatcgatca gcgcgcgatt
    42841 ctgcgccgac tggcggagct gcaatacgct cgtaatgatc aagcattcca gcgtggtact
    42901 ttccgcgttc gtggcgaggt gatagatatc ttcccggcag aatcggatga cattgcactt
    42961 cgcgtggaac tgtttgacga ggaagtggaa cgattgtcgt tatttgaccc gctgaccggg
    43021 cagattgttt ccactattcc acgttttacc atctacccga aaacgcacta cgtcacaccg
    43081 cgcgagcgca tcgtacaggc gatggaggag atcaaagaag agctggccgc cagacgcaaa
    43141 gtgctgttgg aaaacaacaa actgctggaa gagcagcggc tgacccagcg tacccagttt
    43201 gatctggaga tgatgaacga gctgggctac tgttcgggga ttgaaaacta ctcgcgcttc
    43261 ctctccggtc gtggaccggg tgagccaccg ccgacgctgt ttgattacct ccctgctgat
    43321 gggctgctgg tggtcgatga atctcacgtc accattccac aaattggcgg catgtatcgc
    43381 ggtgaccggg cgcgtaaaga gacactggtg gagtacggct tccgcctgcc atcagcgctg
    43441 gataaccgtc cgcttaagtt tgaagagttc gaagcattag cgccgcaaac catctatgtt
    43501 tcggcgacgc cgggtaatta cgagctggaa aaatccggcg gcgatgtggt ggatcaggtg
    43561 gtgcgtccaa ccggattgct tgacccgatt atcgaagtgc ggccggtggc gacacaggtt
    43621 gatgatcttc tttcggagat tcgtcagcga gcggcaatta acgaacgcgt actggtcacc
    43681 acactgacca agcgaatggc ggaagatctt accgaatatc tcgaagaaca cggtgagcgc
    43741 gtgcgttatc ttcactcaga tatcgacacc gtcgagcgta tggagattat ccgcgacttg
    43801 cgtctgggtg agttcgacgt attggtaggg atcaacttac tgcgtgaagg tctggatatg
    43861 ccggaagttt cgctggtggc gatcctcgac gctgacaaag aaggcttcct gcgttccgaa
    43921 cgttcgttga tccagaccat tggtcgtgcg gcacgtaacg ttaacggtaa agcgattctc
    43981 tacggcgata agatcacccc atcaatggcg aaagcgattg gcgaaaccga acgtcgccgc
    44041 gagaaacagc agaagtacaa cgaggaacac ggcattacgc cgcaaggctt gaacaagaaa
    44101 gtggtcgata tcctggcgtt ggggcagaac attgctaaaa ccaaagcgaa gggcagagga
    44161 aaatcgcgac cgattgttga gccggataat gtgccgatgg atatgtcgcc taaagcgttg
    44221 cagcagaaaa tccatgagct ggaagggttg atgatgcaac acgcgcagaa tctggagttc
    44281 gaagaagcgg cgcaaattcg tgaccagttg catcagctgc gtgagctgtt tatcgcggca
    44341 tcgtaacagg atagcgaaga agactgatga caaacggaaa acagcctgat gcgctacgct
    44401 tatcaggcct acattttctc cgcaatatat tgaatttgcg cggtttgtag gccggataag
    44461 gcgttcacgc cgcatccggc ataaacaacg cgcactttgt cttcaatagg aagccggaat
    44521 tttccttcgg atttccgtta acctaaagcc tgtaacgcct tttccagcgc gttatgtaac
    44581 aactggcggt catgacgata cggaatatcg ctggcctcca gtacctcctg gatcacaatc
    44641 cgctctttca ccgccgagac atccactttt ggcccgacga tgaccgcatc aatgaccttt
    44701 ttaccaacat actgctccat aattgccagc ttgctttcca gcttcaaatt agccgcaggt
    44761 aaactcaact cacgccccag attgccgata taaaccatcg gcgctggcgt gcggcgtaat
    44821 gcctgggcga tttccttcag cagcagaatt ggcatcaggc tggtataaaa actgccaggc
    44881 ccaataatga tgagatccgc ttcattgata gcgtgaaccg cctcacgcgt tgcgggtaca
    44941 ttaggcgtta acaataactc ttgaatcggc gtagttaact ggtcgatatt gacctcgccg
    45001 taaacttcat gcccctgatc gtcaatcgcc atcagatcaa caggatgctc tgacattgga
    45061 atcaaatgcg tatccacttt cagcagatta cgaattaaat tgatggcttc cagaggccgc
    45121 acgctaaggt gatccagcgc ctttaacatc aagtttccga gattatgacc ggaaagttcg
    45181 ccattgccac caaaacggta ttcaaacatc gcggaggcga cgctcggttc cgttatcagc
    45241 tggttgaggc agttgcgcat atcgccccag gcaatgccgc cttctgaacg gcgaatacgc
    45301 cccgtcgagc caccattatc ggtggtggtg acgatacccg ttaaacgaga acccaaagac
    45361 gaaagtgatg agagaacgcg tcccagtcca tgccctccgc cgagagcaac gacacgatca
    45421 agatcagcca gcgtacgatt gcgcatatag tttcctggag tcagattatc cgcgctacag
    45481 tagcgcaaat tgcggggaaa cagcaattaa cctgccaata ttgaggatac aaactatttt
    45541 cttctacctc taaaggacga tgcacgctat gcctccctga tgatgtatat caaagtaaaa
    45601 ccgccatttt cccttattct gtagcgaaat agcacgatca tgacgctata tacatgatta
    45661 catagcgaaa gtgtggatgg taaaaatctc atttacacgc tagtatcggc ataaccacta
    45721 aacactctag cctctgcacc tgggtcaact gatacggtgc tttggccgtg acaatgctcg
    45781 taaagattgc caccagggcg aaggaagaaa tgacttcgcc tcccgtatct ggaaaggtgt
    45841 acatggcttc acaactgact gatgcatttg cgcgtaagtt ttactacttg cgcctgtcga
    45901 ttaccgatgt gtgtaacttt cgttgcacct actgcctgcc ggatggctac aaaccgagcg
    45961 gcgtcaccaa taaaggcttt cttaccgtcg atgaaattcg ccgggttacg cgcgccttcg
    46021 ccagtctggg caccgaaaaa gtgcgcctga caggcggtga gccgtcttta cgccgcgact
    46081 ttaccgatat catcgccgct gtgcgggaaa acgacgctat ccgccagatt gcggtcacca
    46141 ccaatggtta ccgtctggaa cgcgatgtgg cgaactggcg cgatgcggga cttactggca
    46201 ttaacgtcag tgtcgacagt ctggacgccc gccagtttca cgctattacc gggcaggata
    46261 aattcaacca ggtcatggcg gggattgatg ctgcatttga ggccggtttt gagaaggtca
    46321 aagtcaatac cgtgctgatg cgtgatgtta atcatcacca gctcgacacc tttctgaact
    46381 ggatccagca tcgccctatc cagctgcgtt tcatcgaact gatggaaacg ggcgagggca
    46441 gcgagctctt ccgtaagcat cacatctctg gtcaggttct gcgtgacgag ctactgcgtc
    46501 gcggctggat ccaccaatta cgtcaacgca gcgacggtcc cgcgcaagtc ttttgccatc
    46561 cagattacgc cggagagatt ggccttatca tgccgtatga aaaagacttc tgcgccactt
    46621 gcaaccgcct gcgcgtttcc tccattggta aactccatct ctgcctgttt ggtgaaggcg
    46681 gcgttaacct gcgcgatctg ctggaagacg atacccaaca acaggcgctg gaagcgcgta
    46741 tttcagcggc gctgcgggag aagaaacaga cccatttcct gcatcaaaac aacaccggta
    46801 ttacgcaaaa cttatcgtac attggcggct aaaacgtcaa aaggagagat cagatgagtc
    46861 aggtaagcac tgaatttatc ccgacccgta ttgctattct tacggtttct aatcgtcgcg
    46921 gtgaagaaga cgatacctcc ggtcactatc tgcgcgattc ggcgcaagaa gcgggccatc
    46981 acgttgtcga taaagccatt gtgaaagaaa accgctacgc tattcgcgct caggtatctg
    47041 cgtggatcgc cagcgacgat gtacaagtgg tattgattac gggtggtact ggcctgacgg
    47101 aaggtgatca ggctcccgaa gcattgctgc cgttgttcga ccgtgaagtt gaaggttttg
    47161 gtgaagtgtt ccggatgttg tcttttgaag agattggcac ttccacgttg caatctcgtg
    47221 cggtagcggg cgtcgccaac aaaacgctga ttttcgccat gccgggttcg accaaagcgt
    47281 gccgtaccgc atgggaaaat atcatcgcgc cgcagctgga tgcccgtacg cgtccgtgta
    47341 atttccatcc acatttgaag aaataagtat gtcgcaactg acccatatca acgccgctgg
    47401 cgaagcgcac atggtggatg tctccgccaa agcggaaacc gtgcgtgaag cgcgggcgga
    47461 agcctttgtc accatgcgca gcgagacgct ggcgatgatt attgatggtc gccaccacaa
    47521 aggcgacgta tttgccactg cgcgtattgc cggtattcag gcggcaaaac gcacctggga
    47581 tctgatcccg ctctgtcatc cgctgatgct cagcaaagtt gaagtcaatt tacaggccga
    47641 gccggagcac aatcgggtgc gtatagaaac cttatgccgc ctgaccggga aaaccggtgt
    47701 cgaaatggaa gcattaaccg cggcctccgt ggcggcgctg accatttatg acatgtgcaa
    47761 agcggtgcaa aaagatatgg tgattggtcc ggtacgtttg ctggcgaaga gcggcggcaa
    47821 gtcgggtgac tttaaggtgg aagcggatga ttaaagttct ttttttcgcc caggtgcgcg
    47881 agttggtggg aacagatgca accgaagtgg ctgcggattt cccaaccgtt gaagcgttac
    47941 gccagcacct ggctgcgcag agcgatcgct gggcgttggc gctggaggat ggcaaattgc
    48001 tggctgccgt taaccagacg ctggtgagtt ttgaccattc gctgactgac ggcgacgaag
    48061 tagctttctt cccgccggta accggaggtt aagatggcag aaaccaaaat tgttgtcggc
    48121 ccgcagccgt tcagcgtagg agaagagtac ccgtggctgg cggagcgtga cgaagacggt
    48181 gcggtagtca cctttaccgg taaggtgcgc aaccataacc tgggcgacag cgtcaaagca
    48241 ttaaccctcg aacactatcc ggggatgact gaaaaagcac tggcagaaat tgttgatgaa
    48301 gcgcgtaacc gctggccgct ggggcgcgtc acggtgattc accgcatcgg ggaattatgg
    48361 ccgggcgatg aaatcgtttt tgtcggtgtc accagtgcgc atcgcagcag tgcgtttgaa
    48421 gccgggcagt ttattatgga ttatctcaaa acccgcgcac cgttctggaa gcgcgaagcc
    48481 acgccggaag gcgaccgctg ggttgaagcc cgggagagcg atcagcaggc ggcaaaacgc
    48541 tggtagtttt ttgttagccg gataaggcac cgctgcgtcc ggcaatttca ccgctcagta
    48601 catgcgttta cgctgcgtcg atgcacagcc tcatcatttt gcagtatcct taagatattc
    48661 cttatatctt caggagatcg tcatggacag attcccacgt tctgattcaa tcgtacaacc
    48721 ccgggctggc ttgcaaactt atatggcgca agtctatggc tggatgaccg ttggcttgtt
    48781 gctgaccgca tttgttgcct ggtatgcggc taattccgcg gccgtgatgg agctgttgtt
    48841 tactaaccgt gtctttttaa tcggtctgat catcgcgcaa ttagcattgg ttattgtgtt
    48901 atcagcgatg attcaaaagc tgagcgcagg tgtaacgacg atgctcttta tgctttattc
    48961 ggcgctgacg ggtcttacgc tttccagtat attcattgtc tataccgctg cttctatcgc
    49021 cagtactttc gtcgttactg ccgggatgtt cggcgcaatg agcctgtacg gttacaccac
    49081 gaagcgcgat ttaagtggct ttggcaatat gctgtttatg gcgttaatcg gcattgtgct
    49141 ggcatcgctg gtcaacttct ggctgaaaag cgaagcattg atgtgggcag ttacctacat
    49201 cggcgtgatt gtctttgtcg gattgacggc gtatgacacg cagaaactga aaaatatggg
    49261 tgagcagatt gatacccgcg acacgtcgaa cctgcgcaaa tattccattc ttggcgcgtt
    49321 aaccttgtat ctggacttca tcaacctgtt cctgatgttg ttgcggatct tcggcaaccg
    49381 ccgttaaatt ctcttcttcc tgctgatccc ggcctggagc cgggattttt ttcggatttt
    49441 ataaagacta ttccgctcat ttacatgaga actgaatcgc ggcaaatgcc agactatgtc
    49501 cactacgtaa tttcagggac agataacgca tttgaacaga caatccactg ctactcaatt
    49561 acagcaaaaa agtgcttcgc tcagcaaaat gctgcttctc atttggtggt taacagcagc
    49621 tggagtatgg gctacggcag gctgtgcctg gatatttcag catttgcatt gggttacgtc
    49681 acaaaagtct ccaggtgcgt ttgtcttcgc tttggctgcg ctgataatag gtggtctctg
    49741 gttaaacaac gccctgaaaa gactgaatgt atcaggcgca tgtctggtct atctgctaat
    49801 aatccttctt gttggctttc tcttcgccag cctttatacc ttttcgagca tagcgacggt
    49861 aatgggaatt acgggaggga tgtttgccgc aatggcgctg atttgctctt gtagtaatag
    49921 ggttatccca ccagtcagac agttatattc atatattttc tgtggtttga gtattgcttt
    49981 tgttgttaac ctgatcctta ccagcagttt ttccgtgtgg ttagcgagta ttttgactgt
    50041 ctttatctgg ggcattacgg ctgcctgtga agcaacaaca ctggaagatt tgatccgtgt
    50101 tgccgacacc tctgaaattt caggttctct tcgctgtatc gttcctgggg caataacact
    50161 ctattttagt attttaagcg tcctctttag aattactgta acagttcttg aatttattaa
    50221 tggtttagtg tggtagtgcg aattgacgag ttttattgcg ttaattggaa tagataatga
    50281 tatttaactt aaggatataa aaataatgaa ttcgcgctca gcaaataaca taactaatga
    50341 ttttcagcac gaagccagga tattaaacag catatggctc attaccgctt taggtttggt
    50401 gacaaccgca ggactggcct gggtagcgaa gtatctcgaa attacggcaa caaaatatga
    50461 ttcaccgcaa atgtatgtcg ccataggatt attattactt tgtatgtatg gcttaagtaa
    50521 ggatatcaac aagataaatg ccgtcatcgc aggcgtaata tatctatttt tactctcttt
    50581 ggtggcgatt gtcgttgcta gtttagctcc tgtacctgcc attatcgtcg tgttcagcac
    50641 tgcgggcgcg atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg
    50701 ttctcaccgt tttatcatta tgatgacgtt gacagggttg gccctggtga tcatcatgaa
    50761 tgccgcatta atgagtgaac gacccgtttg ggtaataagt tgcttaatga ttgtgttatg
    50821 gtcaggcatt atctcgcatg gacgaaataa gctccttgaa ttggcgggga aatgccatag
    50881 tgaagagttg tggagtccgg ttcgttgcgc tgttacaggt gcattaacac tctattacta
    50941 ttttatcggc ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca
    51001 tacgcgtttt tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat
    51061 cgccgcttca tttttcgccc gtagcttctt cgcctggctt tccagtagca ggtaacagat
    51121 cagcgccagc agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat
    51181 aatgctccct ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac
    51241 accgatcccc gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc
    51301 cagtacaaag aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac
    51361 catccagtta acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac
    51421 cagcttttgc cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata
    51481 aaccgcgata atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata
    51541 ccagtgatcc ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata
    51601 gcccagccag ttggtggtaa tactgagcga gaaaatccgc gtaatagtgc tgcccggtaa
    51661 ccctagtcgg gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt
    51721 caggttgaag gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg
    51781 accgcagtaa aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac
    51841 cagcccgacc gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc
    51901 ttcccagtcc acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag
    51961 ccaggtgagg atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcagggtttt
    52021 acccccgtgt tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggcgga
    52081 tcaacctgcg ccagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg
    52141 cgtaaaaagt ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttcggcagc
    52201 atggtctcat cgacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc
    52261 gtctggttaa aatgacgatc gtggatgatg atatttgctt cgagattcag tgacaaactg
    52321 agcggatcga gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg
    52381 ccgtggagcg ggcggcggcg gtactcaaat acctgaacgc cgcctttaac cagatagtta
    52441 tacagcaagc gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt
    52501 ttgatccgca ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg
    52561 aagaagtagg cgttggcgat aatgacttct cgccgcgcct gagtgagcat tttcagatag
    52621 tgacgttcaa tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct
    52681 tctcccggct ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg
    52741 ctctgtccag gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca
    52801 aggcgtaccg cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag
    52861 taattcagcc cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc
    52921 cggcgaaaca cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcgaaat
    52981 accacgccag ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag
    53041 ccatccagca agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc
    53101 agctgtttgc cgacgtcatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt
    53161 gcctcgccaa tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg
    53221 atcttattgc cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat
    53281 gatcagaaag gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat
    53341 ttttgacgta gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg
    53401 ggcgtccgtg gacgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat
    53461 tagctttttg ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta
    53521 gctcattcac ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc
    53581 ccagatgtac gcacatcaca tgaatcgctt ttccggtcat cggtggcaca atgcggcagt
    53641 agagcacgcc gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa
    53701 tgggataacg cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac
    53761 cgtaggcaaa atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc
    53821 agttttccac atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa
    53881 tatcggcgct gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa
    53941 acgcggtaaa gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt
    54001 gatcgggcat aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc
    54061 acaaaaaggt gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga
    54121 taaattcttc aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct
    54181 aagttgctgg cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat
    54241 gagtaagact ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt
    54301 gatgatgctg gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct
    54361 ggaagagctg accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc
    54421 ccgtgtactg gaaaacaact ttttgctggc aattgcaggg ctggcaatcc ttgccggccc
    54481 ggcggtagtg tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg
    54541 cgtacgccgc tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca
    54601 caatatccag actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa
    54661 tagggttaat ccacgtcagg ttttgcagcc atactggcat gttttccacc ggagaaacgt
    54721 aaccggaaag gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt
    54781 gtgtcgagca gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca
    54841 ccatcgtaaa gtagaacagc gccagcgatc cggcgaaggg gatttgatac gcccagatac
    54901 caatcgccag cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc
    54961 cgatgaagat ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt
    55021 cacgttcgcg ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg
    55081 cgatcagcga cggcaccaca aaccatttgt agtcaagatt cgggttatac cagttgcgta
    55141 ccaccagctc gctgttgtta ggtttcggtt ttccttccag cagctcctgc tgataatttt
    55201 tgacgatctg ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga
    55261 ggatcaactg caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga
    55321 aacgcaccag tagtaacgcc ttttgtgtgt cgatggttgg gcggatctcc tgtgggcttt
    55381 tcagcagcag cacatgagta aaggcgctgg cgcgggcaaa acgttgggtc agctccaccg
    55441 aatgctcgcc gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg
    55501 cggcgaacgg gaacagaatc acctgaataa gcacaggtaa aatcagaatc gcgcgggttt
    55561 gcggttcgcg cagcagcgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca
    55621 tgctcttctc cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc
    55681 accgccgaag cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg
    55741 aggaacaggc tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg
    55801 cggatcaccg cgggcatact gtcgatctga aaaataaagc cagagagcat aatcgacggc
    55861 aggaacgcgg cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa
    55921 atcagcagcc ccatccccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc
    55981 agcagcgacc cgcgatacgg cacgccgaga ataaacaccg acaccagcat acacagcagc
    56041 atcgccagca tcccgagaaa gtaatagggg atcagcttac acagcagcag ctccgtgcgg
    56101 gtaatctccg tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc
    56161 agcgaggtga gaatcgcgcc gatgaccgtc atgatgatgg tcaccgcacc ggggataata
    56221 aagtgctggc taatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggc
    56281 tcaaaggtct gcccgttgtc ctccgcccgc tgcatttgcc agatctgcca gatcccttcg
    56341 acatacccct gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc
    56401 ggtgcggtgg cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga
    56461 ccgcgaattt tccccgcctg cattttggcg atcagttcct gacggttatc gctgatggtg
    56521 gcgtcgatgt agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc
    56581 tgttccagca aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa
    56641 ataaacagca gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg
    56701 atctgccgcg tctctttgac gcacagcgcc cgcacgcgac gccaggaaag gatcgggtta
    56761 ctcattgcta tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt
    56821 gggatcgggt tgctcatcgt tagccgactg tgctttcaaa tcgtccggcg taccgctggc
    56881 gattaatttc ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa
    56941 gtgggtggtg accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa
    57001 ttcacggcgg gtgaggggat caacgccgga cgtcggttcg tcgagaaaca gaatgtccgg
    57061 ttcatgcatc agcgagcagg ccagtgccag ccgctgttta aaacctaatg gcagttcatc
    57121 ggtggcgtgg gaggcgatac ttttcaggcc gaacgcctcg ctcatacggg agattttttc
    57181 gttctgcgcc cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac
    57241 cgtcaggttg ccatagagcg aaaacttttg cgccatatag ccgagatgct ggcgcgcttt
    57301 accggaactc tctttcagat ccatccccag caccagcgcc tgaccggagg tcggcaccag
    57361 caagccgcac atcatcttaa aggtggtcga tttccccgcg ccgtttggcc cgagcagacc
    57421 aaaaatctcc cctcgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa
    57481 tttcttggtt agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg
    57541 caatattgca cccagcggcg attccgaggt tccggcaccg cccagcaaat caataaacgc
    57601 atcttcaaaa cgcggcgtag tttcgttgat gttgatttcc ggcatccctt cggcatggcg
    57661 aatatcgtct ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat
    57721 gccgtcgctg acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg
    57781 tggactggtc atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc
    57841 ctgatacagc aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc
    57901 gagatacgag gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg
    57961 ccacagttcg cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac
    58021 tttcggttcg cccaccaggg tacaggccag accgagtttt tgtttcatcc caccggagag
    58081 cttgcccgcc aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa
    58141 agtttgctta cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc
    58201 catcaccgtg agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg
    58261 cagcgcgccg tcgtttttga ttggatcaaa gccaatcacc gtggcattgc cgctgtcggg
    58321 tttcagtaat cccgccagca tccgcattag cgtggtttta cctgcaccgt ccggccccac
    58381 cagccccgtc acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc
    58441 catgcccgga aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg
    58501 cctcgttacc gaattgcacc gtcaccggca ttccctggcg taacgcatca tcggcgtcgg
    58561 tcaccacaat acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt
    58621 tcggggtaaa ttcagcagtc ggcgaaacga aaccaatctg tccgtgatag ggcttgtccg
    58681 ggcgaccatc ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt
    58741 catcaacata ggcacgtacc cacaccggac gcgttagtga aacggtaaac accgtgccac
    58801 cttcattgag aaccgtgcct ggctccaccg cgcgcgttaa cagcgtgcca tcagacggtg
    58861 ctatcaacgt tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt
    58921 cgaggctggc tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca
    58981 atttatcctg tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt
    59041 ccaggtcatt tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt
    59101 tctgcgcata gtcataggcg gcttgagcct gtttcaccgc tgcggcggcc tgagcgattt
    59161 cttcatcgcg atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg
    59221 ctttcgcctg catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc
    59281 ccgctttgat cgcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga
    59341 aactaagatt taccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc
    59401 ggctttgata ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccaatc
    59461 cgatcacgac aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag
    59521 gtcgatatga caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt
    59581 ccagccggta cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat
    59641 caacgcatga gtatggagga tcatgcgggt atcgttggcg tcgcagccgg tccaggcggc
    59701 aatcaggcgt gtcaggtggc tgtgtagcgg gctaatcacc tgttcgtgca ccaggtgata
    59761 ggctgccgtg ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc
    59821 ctgggtgagc agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggctgc
    59881 gcgatcaggc tgtggttgtg cgaacaagcg ttcggcctct tcggcgtgcg gacggaactg
    59941 ctcgccaata aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc
    60001 gaagtagtag gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt
    60061 ggcgttcatt ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc
    60121 ctgttcaccc ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat
    60181 gattgattaa gattatgact ccatagggga gttgtccagt atggctaaga attttagcta
    60241 cgccagtcac agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc
    60301 atgacattgt ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa
    60361 cggtcggggc ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc
    60421 ctgcgcgccg ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc
    60481 cctgcggtgc tggaaggccg cgacctgatg gctagcgccc agaccggtac cggtaaaaca
    60541 gcgggcttta cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg
    60601 cgtcgtccgg tacgtgcgct cattcttacc ccgacccgtg aactggctgc gcagattggc
    60661 gaaaacgtcc gtgattacag caaatatttg aacattcgtt cgctggtggt gtttggcggt
    60721 gtcagcatta acccgcaaat gatgaagctg cgcggcggcg ttgatgttct ggtggcaaca
    60781 ccgggacgtt tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc
    60841 ctcgtcctcg atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc
    60901 gtgttaacaa aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac
    60961 gatattaaag ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc
    61021 cgcaataccg cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa
    61081 cgcgaattgc tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc
    61141 cgtaccaaac acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt
    61201 gcggcgatcc acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa
    61261 tcgggtgaca ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa
    61321 gagttgccgc acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt
    61381 atcgggcgta ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat
    61441 gaacacaaac tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg
    61501 attccgggct atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag
    61561 caacgtggcg gcggcggtcg tgggcaaggt ggtggtcgcg gtcaacagca accacgccgt
    61621 ggggaaggtg gcgcaaaatc tgcaagcgcg aaacctgcag aaaaaccgtc tcgccgcctc
    61681 ggcgatgcca aaccggcagg cgaacaacaa cgtcgccgcc gtccgcgtaa acctgccgct
    61741 gcgcagtaat cttttatgcc gggctatgcc cggcatcagg ctgatgaaca aacgcaaaac
    61801 tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg
    61861 cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc
    61921 agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg
    61981 ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca gtcgattctt
    62041 gcccttacca tgaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt
    62101 tgcgggcgcg gttgccagca agagcgcacg cagttctgca tgctgttcga atttagcgcg
    62161 aagtgctttt cgcatcacct gttctttgac cgactcccag tttttacgca gaggcttaga
    62221 acgatcgcgc cccatgcgtg cggcgaccat aggggaagaa acccgacgga tctcttcgcg
    62281 gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tctgaggtgg gccaggtttt
    62341 cccgtcaacc ttgatgggcc aggcggcaaa gttagaaaaa tcaccgtagt catcgctggt
    62401 gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg
    62461 cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat
    62521 tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc
    62581 tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg
    62641 aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca
    62701 aaacgctggc cggagaagaa gggcgacatc tggcgattga agcccccacc ggcgttggga
    62761 aaacgctctc ttatttgatt cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg
    62821 tggtgagtac cgccaacgtg gcattgcagg atcagattta cagcaaagat ttaccgctgc
    62881 tgaaaaagat cattcccgat cttaaattca ctgccgcttt tgggcgtggg cgctacgttt
    62941 gcccgcgtaa tctgacggcg ctcgccagca ctgagcccac gcaacaggat ctgctggcgt
    63001 ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt gcgaagctga
    63061 agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatactgat atcgctatag
    63121 atgacgatct ctggcgtcgt ttaagtaccg acaaagccag ctgcctcaac cgcaactgtt
    63181 actactatcg tgaatgcccg ttttttgttg ctcgtcggga gattcaggaa gcggaagtgg
    63241 tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctacctgacc
    63301 cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg
    63361 cgctggagat gagtgccgaa atcaccgcgc cctggtatcg actacagctg gacttgttca
    63421 cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga
    63481 tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca
    63541 acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg
    63601 aactgcctga tgaagtgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc
    63661 gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg
    63721 tgcgtctgca tcggttgatt ttgcagatga accgcgcgtt ggggatgttc gaggcgcaaa
    63781 gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg
    63841 cgacgcggga agagcgcgaa gggcagctgc atctctggtt tcactgcgtg gggatacgtg
    63901 tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg
    63961 caaccttgcg ttcgctgaac agtttttcgc gtttgcagga gatgagcggg ctgaaagaga
    64021 aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa
    64081 ttgttattcc ccggatgcgc gttgagcctt ccatcgacaa cgaagagcag catattgccg
    64141 aaatggcggc ctttttccgt gagcaggtgg agagcaaaaa acatctcggt atgttggtac
    64201 tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgcctga
    64261 tgctgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg
    64321 ttgccaacgg tgagcatagt gtgctggtgg gcttacagtc atttgccgaa gggcttgatt
    64381 tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca
    64441 gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg
    64501 tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa
    64561 gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgacc aaaaattatg
    64621 gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag
    64681 gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg
    64741 agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga
    64801 aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgcc catatgctca
    64861 atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctacgt atcaaagggg
    64921 aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga
    64981 cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac
    65041 aggccaacct gacgccgttg ttggcgattc tcctgcacaa actcggtttt cctgtggtgg
    65101 ttcacggggt tagcgaagat ccaacccgcg tgctgactga aaccattttt gaattgatgg
    65161 gtattacgcc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg
    65221 tgtttatgcc agtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc
    65281 ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag
    65341 gtgaggcgct gcgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt
    65401 tctttagcga tatcggcggg cgggcgctac taatgcatgg tactgaaggt gaagtgtatg
    65461 ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt
    65521 atgaaaaaca ggacactgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca
    65581 cggcgcagtg gattgagcgt tgccttgctg gcagcgaacc gattcccgaa tcgctgaaaa
    65641 tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gacggcctgg
    65701 cgcgcgttaa tcaggcattt taattctttt ctcagccgga tgagccatgt catccggctt
    65761 ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccacgc ccggcgtgca
    65821 gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg
    65881 ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga
    65941 ggaacaagaa gcgaaattag tggccgatca tttaatcgcg gcaaacctgg cagggcatga
    66001 ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca
    66061 aattaaccat catgccaaaa tcgttaaaga ggcgggggtg gcagtcacgc tcgatggcga
    66121 tcgcgcattt ggtcaggtcg cggcacatga agcgatggcg ctggggattg agaaagcgca
    66181 tcagcacggt attgccgccg tggcgctaca taactcgcat catatcggcc gtatcggtta
    66241 ctgggcggag cagtgtgcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg
    66301 tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atccgttctg
    66361 tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat
    66421 tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct
    66481 gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg
    66541 ttcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct
    66601 tggcggggcg ctttccggcg gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga
    66661 tgccattctt aactgcatga ccactatcat catcaacccg gaactcttcg gcgcgccgga
    66721 ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgatga
    66781 taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcagaagca
    66841 ggggattcca ctggatgcgg gaagctggca ggccatttgt gatgcagcgc ggcagattgg
    66901 tatgccggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg
    66961 tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg
    67021 tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt
    67081 ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt
    67141 gccggatgcg gcgtaaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac
    67201 cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg
    67261 cggctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa
    67321 ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc gcggcaaaca
    67381 cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa
    67441 ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc
    67501 acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa ataaattgat
    67561 tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg ttttatgccg
    67621 cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa attatactcg
    67681 ctatgcatct cgccagttgt agttaacgta actggctgtc tttcgaacct ctgcgattat
    67741 atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaatg cataagcgca
    67801 cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct tcacattcat
    67861 ccaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac
    67921 tgttgatctg ttcgttgacg gcgtcatcgt tagcccaacc ggatgccata tcgtcctccc
    67981 catatcaatg cttgtacagt taagtgtagc taatccaggg acgaacttgg gcagttcaag
    68041 catcagatct ccgaccattc ccgcagcaga ttatgataaa gattaagcag cgacaggatc
    68101 tcttcacttt cgccgtagcg gcttttcagc gactgaatat tgttgtccag ttcaaacagc
    68161 atggcgcgct ttttatcatc acggatcatc gactggatcc acataaatga tgccactcgt
    68221 acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa cacgagatcg
    68281 cctgccggga gttttacccg atgttgtccg aaggtgtcat taacgaccag ttcgccgccg
    68341 tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtacg catccagccg
    68401 ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc attgttctga
    68461 tagcgattaa acagcggcgt ggaaagggta cgcggcaagg ccgcggcaaa gaataaagca
    68521 tgttggttaa ccgcgttcag cacctcattt tgcagggcgg cgtataacgt gctgcgggtg
    68581 tcgacctgtt gattgttctt aacttgcgca ccttgtgcgc cggtggtgac gcgtccatcc
    68641 acccattcgg cttgttccag ttgttcgcga aaccgggcga cgtcctgtgg cgataacacg
    68701 ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga
    68761 atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt
    68821 agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa acgtttagct
    68881 ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc cagtaacctt
    68941 cggtaaacgc tggcgttccc accgcgccgt ctgaaccttt atgcatactg ccgatatagc
    69001 gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ctggctccat aaggtgaagg
    69061 cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt
    69121 tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc
    69181 cggccacgga tatctcatag ccttcgacac gtttcttacc gtattgcgag taagttccgt
    69241 catcattttg ctcaacttca ttttcgatat cagtacggaa tagcgcggcg gtgagcaaca
    69301 ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt
    69361 taaaatcggt gcggttggca ctgttaccgc tgccagactg cgcaagggcg aagttgttgc
    69421 cgcccggagg ctgctgggaa acggcatagt taatatagac attaccgttt tccgtcaggt
    69481 gatacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg tcgacggtgg
    69541 tgaccggaga accttttgcc acaccagttg ggcaggtgat ggcaccgcgt ccgctgccgc
    69601 cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca
    69661 gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct
    69721 gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaatgctg ctgtcaggat
    69781 gataaatatt taccgcgggt aacgtcaccg gattaacgcc gtagttagtc tgcgtttcac
    69841 gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag aacgtcgagg
    69901 tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg
    69961 accaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc ccgcccataa
    70021 tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg
    70081 tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt
    70141 aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga ttcagagccg
    70201 ctgttcccgc agatggggca gaataacccg gcaaaccgat ggtcggaatg ccgccgtctg
    70261 gcgtgttgtg ctgggtgaca tgcagataat taagatacaa acgattcgct gtaccaaggc
    70321 caaaagcgat agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat
    70381 cgtgcgtttt ttcgcccatc acattcaggc gcaccgcagt ggtatcacca atgacctgat
    70441 tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag gcgtcaatgc
    70501 cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcccgtcggc gcgctgcgcc
    70561 cgtagtcggt gccggaaggc cctttaatca cttcgacctg ctcagtattg aaggtgtcgc
    70621 gcgagacgct gccgatatcg cgaatgccat caatataaat actgttagag gtatcagcac
    70681 cgcgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac
    70741 ccacgccggg gacgtttttg agcgcgtcgg taaggttggt tgcgccctga tctttaatca
    70801 cttgttcaga aatcaccgtc atcgtgcggg tagtatccgc taccggacgc gagaatttcg
    70861 gatcggcaga ttgttgtggc gcataaagcg aaggcgttga tgcttcgaca accagcgtgt
    70921 catccgcgtt agtttgccct tcggcggcga gtgcctgagc cacaggcgtg atgccaatac
    70981 aaagaccggc aaagaacgtg agcgaatgaa attgtctggc agggaaattg cgattgtttt
    71041 ccattttgca ggtgactttt tcttatatgt tgaaattttc actgccacga gaaagcatct
    71101 ctgcggatgc gatgccactg ttaatcccgt gatagtgttc gtgcaagtga taataatttt
    71161 gataagcatt atcacttggc gcgattttct atcacccatt gcgcgaaaaa gaaatacatt
    71221 tatttacatt gagttcatat ttgatgtttt ttgattacaa aacaggcgaa aactgcatcc
    71281 agaggcgaat gcagttacgt tggtggggtt atttgtagat ggttgcagta ccgaacatct
    71341 ggtcatttcc gcctgcggaa ttaatgacat aacctttcgc gccttgctcg cgcgcttttt
    71401 ctgccagttt atcctccaga tcgcttaggt ttgatgcgcc agttgccgaa acggtaccgg
    71461 cgggccgtag ctgaccgctg tccaggttac tcatcggttg cgcggcataa gccgtgagaa
    71521 agattccgct caggacggtg gcaatcagta gtgtgaggca ctttttcata atgacatcct
    71581 cataaataca acgggtagtg cctgataagt gtaggtcgcc tgggcgatga aaatgacgca
    71641 aaaaattgac agagatgtgc ttattttttg aacgatatat ttttacaaaa taagacaaat
    71701 caatgacatg aataatgctg atttttatga tgaattgcca taaaccatag ccatgttaag
    71761 gttttgggga atgataaacg cagccagata tttaccctgt cgcgttatgc gggtatctta
    71821 cgccgctgct taaaggagaa tgctatgtcc gcccagaaac cggggttgca tccgcgcaac
    71881 cgtcatcaca gccgctacga tctcgccacg ctttgtcagg tcaatcctga actcaggcaa
    71941 ttcctcacgc ttacacccgc cggggagcaa agcgtagact ttgccaatcc gctggcggtg
    72001 aaggcgctca ataaggcgtt gctggcccat ttttacgccg tagcgaactg ggatatcccc
    72061 gacggttttc tctgcccacc agtaccgggc cgggcggatt atattcatca ccttgccgat
    72121 ttactggctg aagcaagcgg aacaattcca gcgaatgcca gcattctgga tatcggcgtt
    72181 ggtgcgaact gtatttatcc gctgattggc gtacatgaat atggctggcg ttttaccggt
    72241 agtgaaacca gcagccaggc gttaagcagt gcgcaggcga ttatcagtgc taatccgggg
    72301 cttaaccgcg ccattcgtct gcgtcggcaa aaagagagtg gggcaatttt taacggcatc
    72361 atccataaaa acgagcaata cgacgcgacc ttgtgtaacc cgccattcca cgattccgcc
    72421 gctgcagcgc gggcaggtag tgagcgtaaa cgccgtaacc tggggctgaa caaagacgat
    72481 gtactgaact ttggtggtca gcaacaggag ttgtggtgtg aaggtggtga agtcgccttt
    72541 atcaaaaaga tgattgaaga gagcaaaggc ttcgcgaagc aggtgatgtg gtttacctcg
    72601 ctggtttctc gtggtgaaaa cttaccgccg ttgtatcgtg ctctgacgga cgtgggcgcg
    72661 gtgaaggtgg ttaaaaaaga gatggcccag gggcaaaagc agagtcgctt tattgcctgg
    72721 acctttatga acgacgagca gcgccgccgt tttgttaatc gccaacgtta aagcgttggc
    72781 tcccccggcg gcagcggttc agccggggcc gcgcccggag caggcagcac ctgataagtc
    72841 tgcactggcg cgcgaacacc tgccaggtcg aaatgctttt tcacctggct gtcgagggca
    72901 aagcggaccg tccactgttt gagtggcagc gtggtgaacg aaacacgcag tgtaaacgcg
    72961 gtattgctta agccgacaat cccggcaaag tttggttcac caataaccag cccgcgaatt
    73021 tcttcgtttt ccatcaattc cgctaccgca tctttcagtg cctgattggc tttatcagca
    73081 tcttcatggc gatcaacatc gtagtttgcc accaccgaac caatgccgcg gacgaagtta
    73141 gcaaaggtgg ttatcgaaga ccacggaatg atgtgatacg ccccggtatc ctgacgcacg
    73201 cccacggagc gaatcgacat ccgttccaca gtgccggtca acggcccgat agtaaccaaa
    73261 tctccagtgt tcatgccgtt ttcaaactga ataaataccc cggtgataat atctttcacc
    73321 agcgtttgcg aaccaaacga gatagccaga cctaatgccc cagcacctgc cagcaatggc
    73381 gcgatattga cgccgatttc cgacaacaca atcatgatgg tgatggtact gataatcacc
    73441 gccagcgcgt tacggaacag cgtcagcagg gtacgcgtac gggcgctggg tagcgggcgg
    73501 ccatgaatat ccgaagccag ccggttttcg atcaaactgg cgagcactgt ccagccaacc
    73561 gccgagaaga aaagaatgag tgcgatgcgg atcaggatat ctacggtttt ctgccccgcg
    73621 ccgttttgca gccagttcca gaaatcgaac aatccccatg cgctcaacag caacattacc
    73681 gccacgcaga cggtcagaat acgcgccgtt ttcagcgccg ccgaaagcca gccattcagc
    73741 cgtttttgta gctccggata gttacgctga gtatgtggcg agagggtgat ggttttcgcc
    73801 agccagcggg aaaacatacc ggaaacaaac gctgcgatac caataatcgc caggctgcgc
    73861 accgttgcac ccatcataaa tttcaggctg ttgcccggat cgaacaacga aaagaaaaac
    73921 agcacgataa aataggcgct tgccagccag tgccacacca gcgcaaaggc gcggataaac
    73981 aggctgaaaa aggccagcga atgctccgca aagttgagca aatgttgggt aatctctttt
    74041 ttattgcgaa agatcaggta caacgcccag acggtcatgc acagcataat gatgacgttc
    74101 gccagcgcac ctatctgtac attcacctga ttagagataa tcggcacggc cacaatcagg
    74161 ccataaccta tcaaactgct taaccagctc aggcgacgac tccagtaacg ggcgctctcg
    74221 tcctgaatcg tgaacgggcg cagttcagcc acgtttgggc aaaaaatcag gcgtagtacg
    74281 gctttgaaaa attcaatcag ggcaaaggca ttgagaaaca aactttgttg gaaagcgatg
    74341 gtgcgactgc ctgcattcag gttgtcgctt aatacctggc cgacaaataa tgtcagggcc
    74401 agtaacagca ggtcgataat aaacgccccg ataatcatcg ccggaagctg caaccagttg
    74461 ctgcgctcac gatttttttg cctcgcccac tgacccattt tgcgatacag cggcagtgcg
    74521 cacaggcgaa tcagccagta aaaaccaaac actaataccg ctaacattga aaaatgggtc
    74581 agcgcattgc tgaaggtttg tggattaaat ggcttatgcg gggagccggt gatattgcga
    74641 taaagttgcc cgaagcgggc ggaaagggct tcaccataat ggcggctgac ttcggtgacc
    74701 ttttgcagca cggtttgctc ttcaaccagc gtcggcggca cgatcttcgg gaccggttcc
    74761 gcagggggcg tcgcggcgac agtacgcaac tggtcgatca attctttacg cgaggtgtcg
    74821 ttatccagca catccgccag tgcgccatag gccgcttttt tttgttcgat atccggttcc
    74881 ggggccggtt cagtcgttga gtccgttgtc gttgtggttg taacgccggg tatggatacc
    74941 gcgtgggcag gtgcgcccag caggcagaag aggatgaaca ggatccaccg catgactcct
    75001 ccagtgagaa aacagagcaa aaagataagt atagatgctg ggggaggggc gattttaaat
    75061 gagaggaatc tggtgtgcct ccctttcggg tgaaagggag gcagagaagt taagagacgt
    75121 gctgcaaaaa ttcctgcaag cgctggctcg gcgggttctt gatcaatacc tgcggatcgc
    75181 catcttccgc aatccggcct ttgtcgataa agatcagccg cgaagctact ttctcggcaa
    75241 aaccgatttc gtgggtcacg atcaccatcg tcatcccttc ttcagccaga tcctgcataa
    75301 ccttcagcac ttcatggcgc agttccgggt caagagcgga agtcggttca tcaaacagca
    75361 tcattttcgg cttcaccgcc agcgcgcggg caatcgccac acgctgctgt tgaccaccag
    75421 aaagttcgga agggtagtga tgtgcacgtt ctgccagacc gactttcgcc agcagctcac
    75481 gcgccagttt ttccgcctct tctttgttcg cgccacgcac gcgtagcgga ccaaacatga
    75541 cgttttccag cgccgtcaga tgcgggaaga ggtaaaactg ctggaacacc atacctgctt
    75601 cctggcgaat caggcgctcg tcaactttcg gatcgttaac cttcaggcca tcgacaatca
    75661 gatcgccgga ggtgatttct tccagtttgt tgatgcagcg cagcagggtc gatttaccgg
    75721 aaccggacgg cccgataatc accacgactt cgccctgggc aatgttcaaa tcgatattgt
    75781 gcagcacctg ggttgggcca aagtgcttgg agacgttttt aaattcaatc acaggatttt
    75841 catccttctt tccagacgac gcagaataaa gctcagcacc agggtaataa tcagatagaa
    75901 caccgccacg gcgctccaga tctcaagggc gcggaagtta ccggcaataa tttcttgccc
    75961 ctgacgggtc agttccgcca cgccgatcac aataaacagc gaggtgtctt taatgctgat
    76021 gatccactgg ttacccagcg gcggcagcat acgacgcagt gccagcggta aaatgacgta
    76081 gcgaatggtt tcccaacgtg aaagaccgag cgccagtcct gcttcacgaa aacctttgtg
    76141 gatagacagc accgcaccac gcgtgatttc cgcaatgtac gcgccggagt tgatcatgat
    76201 ggtgaccacc gccgcagtaa atgggtcgat gcgtaagtcg ttaaacgcca tcggcagggc
    76261 gaaatagata aacatcacct ggacgacgat aggtgtgccg cggatcactt caataaagac
    76321 cagcgcgacg tggttggcta tccaaccacc gaaggtgcgt gcaaaacctg ccagcaatcc
    76381 gattaccaga ccgcctgcca gaccgaggac cgaaatccac agggtcattt tggcaccttc
    76441 aatcagaagc ggaatggcag gccagatggc actccagtca aactgcatat gttgttcctg
    76501 ttaccgtggt gtaaataatc aaattcaggg taattaatgg ccccggagcg ggtttgcgcc
    76561 cctcacccta atcctctccc cagaggggag agggaactgc cagagcgttt tacaggtgta
    76621 gcgttattat ttcggttcag taccgaacca ttttttgtag atttcgttgt aagttccgtt
    76681 ctcgcgcagg gttttcaacg cgccgttgac tttgtcacgc agctcgtcgc tacctttcgg
    76741 gaacgcaata ccgtattgct gcgcttccag agagtcacct accgctttga actgaccgtt
    76801 accggcggtt ttgatgaagt acagaatgtt tggcgtatcg tgcagaacgg cgtctgcgcg
    76861 gttggtgccc agttccatat aggcgttatc gatgttcggg aactgacgca gatctttagt
    76921 tttgatgttt gctttcgcgt aatcaacgga gccagtaccg ctcttcacag caaccacttt
    76981 cccgtcgaga tctttcacgc ttttcacatc gttattgtta gctttcacca tcactaacag
    77041 gccgcttttg tagtagccgt cagagaaatc gatcgctttt ttacgctcgt cggtgatggt
    77101 aatgcccgcc agcgccagat cgacgttttt ggtttgcagt gccggaatga tcccactgaa
    77161 atccatcggc ttcagttcgt aatccagctt cagctcttta gcgatggccg cccacagatc
    77221 aacgtcaaag cccacatatt tatcgccctg tttaaattca aacggaacga aggcggtatc
    77281 cgtcgcgaca actaattttt tatccgcggc atgagaagaa accgcaaaag ccagggtcag
    77341 tgcagccagt gaaactttta atacagactt catagcattt ccttttttct atccacggga
    77401 cgatcccctg cgtgacattc acatatatga aaaaatcgtg ccagttttgc aactccttga
    77461 tttttcgaga tgcggcaaaa acgtgatgca cgatttatag ggcaataccc tgaagatgca
    77521 ccattctggg gcaccaatct ggtgcgctaa aattgtgcac tcaacacagt gattatttag
    77581 cgtaaaacct gatgaaaaaa caatctttat gtaacgattg tgtgatgatg tggatacaaa
    77641 aaatttactt aatcagctgg agatagcaga tggatgcact aaataagtgc gttgaggtgg
    77701 gctgcaaaac aaaacggcct cctgtcagga agccgctttt atcgggtact aaagttctgc
    77761 accatcagcg atggatttat tcgatgttag actcgataaa ccacaggaat ttatccaggt
    77821 cgcgagacgc ggcggtcagg atatctgcgg tgtcgtcatc tttcgcttcg ccaatcgctt
    77881 tgcgtacgtc attagcgacg attgcgtaac ggtcagccag ttctttcagg tgatcctgaa
    77941 cgttgtggat gtccagcggg taacttttca gcggggtttt gctgttgata acttgagtgg
    78001 tccccagagc tacaccgccc agctgcactg cacgttctgc catggtatcc agatgatcga
    78061 tcagtgcggt gcggaagcca tccagcattt catgtacggc aatgaagtta gcgccgcgca
    78121 tgttccagtg cgcttgtttg gtaatcaaag aaagatcaat aaactggata acctggcgat
    78181 tcagcaactc tactgttgct tttttctcgc tgtcggagac atcgttgcgg gtataaagca
    78241 gattggtcgc ttttgattta actaatttag cggtactcat aatttcatat cctcttgatg
    78301 ttatgtccca gtaattaacg agactaagta tagcaccggc tatgtgttcc gctattctgg
    78361 ctgttcctat cacactaata gtggtaacaa gcgtgaaaaa caaaactaat aaagattcaa
    78421 tgagttagat atattgataa gaacaattct tatttatctg ctcgccagga attttcggaa
    78481 taaatatgct tttgcataat tcgatatata aagacggtgt agaggaaaag tagcgagaaa
    78541 ttctgcatgg ttatgcataa ccatgcagaa atgtaaattt aattaatgtc taattctttt
    78601 attttgctct ctttgcgtac tgtcagcgta gaccccattg aagcggcgat gatagcgccg
    78661 agcgccagca gctgtatggg tgtcagtgtt tctccgagga aaatcatccc ggaaacggca
    78721 gccagcgccg gttccatgct catcagcgta ccaaatgtcc gtgttggcaa acgggtgagg
    78781 gcaatcattt ccagcgaata aggcagagcg gtcgagagaa tagcgacagc cagacccaat
    78841 ggaataaccg accagtgcca gagtgcttca ccagcctgaa gcgctccaat tggcacgaaa
    78901 attaacgctg caatcaacga accaattgcc accgtcgcag ggccatgttc cgctcctgcg
    78961 cgttgcccac ttaaaatgta aatagcccaa caagccccgg cccccagtgc cagcgcacag
    79021 ccggttaaat cgacatggga aacgtcttgc cccagcggta gcaggaacca cagaccaaga
    79081 accgccagca caacccagac gaaatctacc gggcgacgag aagagaacag cgccaccgcc
    79141 agtggtccgg tgaactccag cgccaccgca atacccagcg gtactgtctg aatagaaaga
    79201 taaaaaagat aattcatccc acccagcgaa acgccgtaaa acaacagcgg taaccgttgc
    79261 tctttggcaa agcgcagtcg ccatggctta aagaacgcga tgaggatcag cgttcctaat
    79321 gccagacgca gcgcagtgac acccggtgcg cccaccagag gaaaaagtga cttagctaac
    79381 gaggctccac cctgaataga cgccatggca acgagcaata tgattattgg taaccagacc
    79441 ggcattttac gtaatgaacc aggcatcctt tctcccacaa atatctagac ttaagtaaag
    79501 cgtggagtgt actggatata cccaatgctg gttgagcatt tgttgaaaaa attttccccc
    79561 gttttgacta aaatgcgcca ggattgatgg aatcattagt ctggtgatta ggaataatct
    79621 ggatgaatga cagggaaaac atgcgtaata cttacgcagt tctctgaaaa agtgatttaa
    79681 atttagatgg atagcggtgt atggaaacgt tctgttacat gaaatggccc gttagacatc
    79741 acaaatcgcg aagagtttcc cattaatttt tgatatattt aaaacttagg acttatttga
    79801 atcacatttg aggtggttat gaaaaaaatt gcatgtcttt cagcactggc cgcagttctg
    79861 gctttcaccg caggtacttc cgtagctgcg acttctaccg taactggcgg ttacgcacag
    79921 agcgacgctc agggccaaat gaacaaaatg ggcggtttca acctgaaata ccgctatgaa
    79981 gaagacaaca gcccgctggg tgtgatcggt tctttcactt acaccgagaa aagccgtact
    80041 gcaagctctg gtgactacaa caaaaaccag tactacggca tcactgctgg tccggcttac
    80101 cgcattaacg actgggcaag catctacggt gtagtgggtg tgggttatgg taaattccag
    80161 accactgaat acccgaccta caaacacgac accagcgact acggtttctc ctacggtgct
    80221 ggtctgcagt tcaacccgat ggaaaacgtt gctctggact tctcttacga gcagagccgt
    80281 attcgtagcg ttgacgtagg cacctggatt gccggtgttg gttaccgctt ctaatcactt
    80341 tggtgatata aaaaatccgc ctctcggggc ggatttttgt ttttaaggct tcgggtcgaa
    80401 aatatcggtt ccgagatggt tgtaatccac cttctgtaac tggaagttgg tgatgtatat
    80461 cgacccggct ttcttctcag atataaacgg gtatttgatg ttaatttcct tcgctttaat
    80521 tcccgtccac tgggagaaaa aacctaagaa gtcattggct gagcggcggg ctttaatcac
    80581 ccgatgcgct ttatcgtcgc tggaaatgac cataaaaggc acctggaaat tttgctgata
    80641 cttatcatca tgggcaaggt attgcacgtc tttgccgcgc tctttaaagg ccagaccgtg
    80701 gtcagaaaag taaaccagcg agaagctgct gccgctgttg cgtaactgat cgtacagctt
    80761 gcgcagtaaa tcgtccgttt gcgtcatggt atagagatag cacgacgttt ctttcgactg
    80821 cacaaaggtt tcgtatttgc cttgtgtcct gtcgcaggcc tgcggatgtg agcccatcag
    80881 atgtagaaca atcagctgcg gttgcgagtg ctcttgcgcc agcacttgag cggtcatatc
    80941 cagtaacgct tcgtctttgg tatttttatc tgcttcaaaa ttaccttctt tcaggaagta
    81001 cacttcatct gctcgtttgg cgatgctggc gatagcagta tcgtattcgc cgatttggcc
    81061 ctggttggaa aaccaccagg tctggaagcc cgcgcgattt gccagggtga caaagttatc
    81121 ctgaaactgt ggtttgccat cgactacgcg attgagcgtt aagccaagcg atttctgcgt
    81181 ggagccactg gcggcaatgt agtcagcaaa tatcaaaccg ttaacgctgc tggcaaacgg
    81241 ggtattgtcc cagtgaccgc caaaggcacc gagggcatcg cgacgcgcgc tttcaccgat
    81301 caccaccaca taggtctgat acttcggctt aacggcagtg accgtccagg tatctttcat
    81361 tccggaaagt tttgccatcc gttcttgctc ttcaatcacc tcgttattat tgacgatgac
    81421 atccttagca aaacgaaata ccggatagcc agtatctttc agtttaaata cgccgcccca
    81481 ggccaggttt tgcacgggag caacaaaaaa tgtcgccaca ctgaatacca gacacaggct
    81541 gtcgaaggta ttccagcgcg ctttttcact ctcttttttt cgcctgattg ctattacgcc
    81601 gagcacaaaa ataaatatgc cgaccatgta gctgtaccac ggaaaaatcg tcaggatttc
    81661 ggtcgactct ttcatattgg tggagtgcaa tgccagcaat gtattgaagt taggcgcgcc
    81721 gtaggcctga gcaaaaggga aataacaagc cgccaccagc gaactgatac cgaccagaac
    81781 tttttgtacg cgaggcaatg ttcgccatag caaaagcaaa atagccgtaa acgcagcggt
    81841 gtagagcaaa ctgaaggggt aacctaagcc gaggttaatt aatagcgact gtaaaaagta
    81901 gaacgcagtc cacgggctaa atacccggct acgggtaaca agcgattctt tgagggttaa
    81961 attcatatgc cactatcgta acaaacgcca tgtgcttacc ctggcgtcaa gggtcaatac
    82021 ctgcaagagt gcgtgaagag tggaaagggg tccgcatccg cgagccgcaa tatacgcagg
    82081 gctgcaagaa gatagagcga gccgccgttc aggtcaacta ctgacgtaca aatcttttgc
    82141 gaaggggatt gcaaatccga caaaaagtgg ggtttttcag accaaaacgc gttatgcctg
    82201 catgaaagca ggagtgaagc ggcgtaccgc gacgccgcta tttatcggaa ggtttatctt
    82261 gctgcggttt gttgttgacc atatcgcaca acatagagag cagcattaac cgtactttaa
    82321 agggagaatg actaaacacg cgcatacacc tcttgaactc attcataaga cctcctgact
    82381 tgctaatccc gtcgatcctt gagggatgat tgcattacat acagatatag cacaggctat
    82441 attatatagc tattgctaaa acgttaattt tttgtacctt cgcaactctg gtttacaatg
    82501 tgcgcacgaa atgagaacgc tatgtatgcg tcaccataat gaggaagcac aatgagtcgt
    82561 cgcgcaggta cgccaacagc aaaaaaagtg acgcagttag tgaacgtgga agagcacgtt
    82621 gaagggttcc gccaggtcag agaggcgcat cggcgcgagc ttattgatga ttacgttgag
    82681 ctgatttctg acttgatcag ggaagtgggg gaagctcgtc aggtggacat ggctgctcgt
    82741 ctgggagttt cgcaaccgac ggtggctaaa atgcttaagc ggctggcaac catggggctg
    82801 attgaaatga tcccctggcg tggcgtgttt ttaacggcag aaggagagaa gctggcgcag
    82861 gaaagccgcg agcgacatca gatagtcgaa aatttcttgt tggtgttggg cgtcagtccg
    82921 gaaatcgccc gtcgcgacgc ggaaggcatg gagcaccatg ttagtgaaga gacgctcgac
    82981 gcttttcgtt tgtttaccca gaaacacggt gccaaatgag cctgcctttt ttacgcacgc
    83041 tgcaaggcga tcgttttttt cagttattaa ttcttgttgg tatcggatta agctttttcg
    83101 tgccctttgc accgaaatcc tggcctgctg ctatcgactg gcacaccatc atcaccttaa
    83161 gcggcctgat gctgctgacc aaaggtgtgg agttaagcgg ttattttgat gtgctggggc
    83221 gcaaaatggt gcgccgcttt gctacggagc gtcggctggc gatgtttatg gtgctggcgg
    83281 cggcgctgct ttctaccttt ctgaccaacg atgtcgcgct gtttattgtt gttccgctga
    83341 ctatcacgct aaaaagactg tgtgagatcc cggttaatcg gctgattatt tttgaggcgc
    83401 tggcagtcaa cgctggttcg ctactgacgg caattggcaa cccgcaaaat attcttatct
    83461 ggggacgttc tggtctttcg tttgccggat ttattgccca aatggcaccg ctggctggcg
    83521 caatgatgct gacgctcctg ctcctgtgct ggtgttgttt ccctggaaag gcgctgcaat
    83581 accatacggg ggtgcaaaca ccggagtgga aaccgcggct ggtgtggagt tgtctggggc
    83641 tgtatatcgt ctttctgacg gcgctggagt tcaaacaaga gctgtgggga ctggtgattg
    83701 tggcggcagg ctttgcgctg ctggcacgtc gcgtggtgct cagtgtggac tggacgctgc
    83761 tgctggtgtt tatggcgatg tttatcgacg tccatttact gacccagctt ccagcgttgc
    83821 aaggcgtgtt gggtaacgtg agtcatctat ctgaacccgg gttatggtta acggcaatcg
    83881 gtttatcgca ggtgatcagt aacgtgccga gtaccatatt gttgctgaac tatgtgccgc
    83941 cgtctttatt actggtatgg gcggtaaacg taggtggctt tgggttatta cccggatcgc
    84001 tggcaaattt gattgcgcta cgtatggcga acgatcgccg catctggtgg cgtttccatc
    84061 tctattcaat accgatgctg ttgtgggcgg cgttggtggg atatgttttg ttagttatac
    84121 tcccggccaa ctagggtctg gcaaataaaa aaggcggatt atgagtccgc cttttgcttt
    84181 aagttttgaa gattaattca gacgaaccgg catcccggag cggtttttaa tcgcttcatc
    84241 aagaacaacc tggtcaacat ctggctgacc ggtcactgtt tgcacgctct tcgtcagggt
    84301 aattggcaca atttcctgac cttcaaactg ggcttcggtg gtagacagcg ggttatggac
    84361 ttcaatataa cggctgccgt ctggctcggt ggtcgctttt accggctcat caataaactg
    84421 tacgcgggta ccgaccggta ctttctcgaa caggaatttg atgtcttcgt tacgcagacg
    84481 cacacaacca tgacttacac gcaggccgat accgaagttg gcgttggtgc catggatagc
    84541 gtacaggcga ccgatgtaga gtgcatacag ccccatcggg ttatccggac ctgccggaac
    84601 gacagccgga agcggttcgc ccgcagcgcg gtactctgcg tgcattttgg cggtcggcgt
    84661 ccaggtcggg cctgctttct tacgctcaac tttagtggtc cagttgatag gcgtatcttt
    84721 gcctaactga ccaatgccga tcggcagcac gataacggtg ttggtccctt tcggatagta
    84781 atacagacgc atctctgcac tgttaatgac gatgccttca tgaacggtat ccggcaggat
    84841 cagctgctgc ggaatgttca gtacagtacc gcctttcggc aggaaggtat ccacacccgg
    84901 gttcgcttcc atcatattgg aaagccccat ctggtactcc gcggcaaaat actccagcgg
    84961 ctgagtgtta ccttcaggaa tggtgatcac ctgattctga ccaaccaggc gactcccgtc
    85021 ggttggcaga ggataagtta ccgcagaggc ggtactgcaa aagccgacaa cagcgaaggc
    85081 cgctgcgaat aatgttttca atttcatatt catgttaagc gagattttgt gcctggcagg
    85141 ccattgggtt gagaatatta gagtattgga agcgcattat aagttcattc cagctcacag
    85201 tgaaatcaga tgtgtacgaa atcacatttt ttgcctttgg cttgagtgta gaccttaagc
    85261 gaggagcagg atcttctttc agacttatgg cataatgcgc ggtttgtcat atctcttttc
    85321 aggatacgcc tgtgttagtt tccagtaacg tcaccatgca gttcggcagt aagccgttgt
    85381 ttgaaaacat ttccgtcaaa tttggcggcg gcaaccgtta cggcctgatt ggcgcgaacg
    85441 gtagtggtaa atccaccttt atgaagatcc tcggcggcga cttagagccg actttgggta
    85501 acgtttccct cgatcccaac gagcgcattg gtaagctgcg tcaggatcag tttgcctttg
    85561 aagagttcac tgtgctggat acggtgatca tggggcataa agagttgtgg gaagtgaagc
    85621 aggagcgtga ccgcatctat gctttgccgg aaatgagtga ggaagacggc tataaagtgg
    85681 ccgatctgga agttaaatac ggcgaaatgg acggttactc tgcggaagct cgcgccggtg
    85741 aattgttgct tggcgtggga attccagtgg aacagcacta cggcccgatg agtgaagttg
    85801 ctcctggctg gaagctgcgt gtgcttctgg cgcaggcgct gtttgctgat ccggatattc
    85861 tcctgctcga cgaaccgacc aacaacctcg acatcgacac cattcgctgg ctggaacagg
    85921 tgctgaacga gcgtgacagc accatgatca tcatctcgca cgaccgtcac ttccttaaca
    85981 tggtctgtac ccacatggcg gatctggatt acggcgagct gcgcgtttat ccgggtaact
    86041 acgatgagta catgacggcg gcgacccagg cgcgtgaacg tctgctggcc gataacgcca
    86101 agaagaaagc gcagattgct gagttgcaat ctttcgttag ccgctttagc gccaacgcct
    86161 cgaaatctcg ccaggcaact tcgcgcgcgc gccagattga taaaatcaaa ctggaagagg
    86221 tgaaagcctc cagccgtcag aacccgttca tccgttttga acaggataag aaactgttcc
    86281 gtaacgcgct ggaagtggaa ggtctgacca aagggtttga taacggtcca ctgtttaaaa
    86341 atctcaacct gctgctggag gtcggtgaaa aacttgcggt actgggtacc aacggcgtcg
    86401 gtaaatcaac gctgctgaaa acgctggtgg gcgatctgca accggacagc ggcaccgtaa
    86461 aatggtctga aaacgcgcgc attggttact acgcccagga ccacgaatat gagtttgaaa
    86521 atgatctgac cgtgttcgaa tggatgagtc agtggaagca ggaaggcgat gacgagcagg
    86581 cggtacgcag cattctcggt cgtttgctgt tcagccagga cgacatcaaa aagccagcta
    86641 aagtgctttc cggtggggaa aaagggcgga tgctgtttgg taagttaatg atgcagaagc
    86701 cgaacattct gatcatggac gaaccgacca accacctgga tatggaatcc atcgagtcac
    86761 tgaacatggc gctggaactg tatcagggca cgctgatctt tgtttcacac gaccgtgagt
    86821 tcgtaagctc cctggcgacc cgcattctgg aaatcacccc ggaacgcgtg atcgacttta
    86881 gcggtaatta cgaagattac ctgcgtagta aagggatcga gtaagtgatt atccgtcacc
    86941 ccgatactgt cggggtgacg gcggctgatg acaaatgcaa aatagcctga tgcgctacgc
    87001 ttatcagtat ctacgcagta cctgtaatat attgaatctg caggactttg taggccagat
    87061 aaggcgttaa cgccgcatca ggcattaaga aagcgcactt gtcagcaatc tgagtaacgt
    87121 cgaatcgatt ttcttaatcc cacatcaatt cacttccatt aactctcaaa catccatgcc
    87181 caacgctcgc ttaccgtgaa tgttgaggtc ggcaagcgta aagcgtcctt cccagtttgt
    87241 ttcatagtcc tcgcgcggga agtcgcccgg cgatgcgcct ttttccagtg cggccttcac
    87301 cttgtgcgcg taggcaagat ttttctcgca catcggtgcc gcaggaatgt acatcacgtt
    87361 gccccaaccc tgttgatttt caacgggggc aacggaatgg atgacgtcgc agtgccacca
    87421 gacggagtct ccggcctcga gttttggaat gctggttaac gcctcaatta acagtggatg
    87481 ccattgctct gataccggca acactcttcc gggcgctacg ccgcacagtt catcctccgg
    87541 cacatcatca agcagcggac gtaacagtac gtacgccatg gcttcaggaa tgggcacaac
    87601 gtgcagcagc ccctgaccag gcagcatatc agagagcgct gtccagccct ggaatgtccg
    87661 aaacacggaa catttggtgg tgttgtccac cgtgtactct tcaacttccg tacgatgtgc
    87721 cgcatgccag ggatcatatt gcgccagatt gccattaaag acgttggcga aaacgcgctg
    87781 atacgctgga agcagccagc gttccagcgc cccggagtcg gtatgcgctc caagaccttt
    87841 ggaggtcgtt ccgggcggac ggcggcggat acggtcaggg tagatgacgc tcacatccgg
    87901 gttaaaccat tgctttccat cactttcaaa tgtccacaga cgattgagaa acgactgcgc
    87961 attcgccatt tcttcactct ggcgggcctg catttgcgcc tgcgaccagt agatggggta
    88021 aatctcggga cgtgaagcgc tgagcgtccc gaagaaatta tcgccggggc ctttgtagac
    88081 ctcgtcaaag cggttgcggt ccagatagtc cagcatcgac tgatcccagc ctagcgcttg
    88141 ttcgcggggg aaatggcctt ttatcaccgc acaaccgcga cgtttaatct gttcgcgctg
    88201 ctctgcagta acatgacctg ctttgatatc ggcataagac agcaccggcc agacggcatc
    88261 gccctgtgct ttgagtgcgt tgatttcagc cactcgcgtg gcaatgtcat cgcttagctg
    88321 attaaagatc tgctggacgt cgccaagctg cgcccgcagc gcgtgcttca tctgacggat
    88381 agctgctttg tgatcggcag gcaatgtgtc gctggtaaaa gtagaagcca taaccacctc
    88441 tcattaactt tcattcgaaa ccattctgat ttgcatgtga caatataagt tataaataag
    88501 ttaatgcaag tttaatgatt tgatgtgatg tacaggatgt aaaaagaagg gaaaaccgga
    88561 gcgcacggct ccggtagaga ggtcagctgt taaaagggga tgtgttatcc agcaccgcct
    88621 gaatcacatt cagcgcgcct tcatgattat tatcatcggt agcgtaacgg gcgatttgtt
    88681 taatgttttc cgcagcattg cccatcgcaa aggaataacg cgccattttc agcatctccg
    88741 catcgttacc gctgtcgcca atcgctacca cattttgcgg tgacagatcc cagcgtttca
    88801 gtaaccgcga aataccgttt gctttatgta gaccgggaat aatcaggtcg ataaagccaa
    88861 aaccactggt gacgggtttc ataatgccat cgagcgctat gtgcagtttg tcgatcacta
    88921 acgggatttg ttcatctggc aggttgagcg aaaacttgaa cagtacgtcg tcaatctcct
    88981 gataatcttt tacagctttc aggcgatggt agtgttttgc catcagtgcg acaaatgctt
    89041 cgggggcatt ttcgctgaca tatgcacttt gcagaccgca ggcgacaaaa ttgagttgct
    89101 tatcttttag caactcgcca ataacaatcc gcgattcatg tcgggtcagt tcgccgtgga
    89161 acagttgctt gccatgttcg taaaccagtg cgccgttttc cgcgacaaaa gagatctcat
    89221 cctttagctc aggaaagaac gaaataagct ggtaatactg attaccgctg gcgacaacga
    89281 acttaatgcc gcgctttttc agttcctgat attgcgccat aaaatgtggt tggttgtacg
    89341 ttttggcgtc gttaagaaaa gtaccgtcca tgtctgtgac gataactttt acgctcatag
    89401 gtgtgctcct ggctcgaaaa tgaaaccgta acagtgtaat aacaatgtga cgcagagcac
    89461 aaattatatt tcgaatgaaa gtaaggatga aattgatgat gtgaatgatt tagcccggcg
    89521 acgacgccgc cgggccgagg agattacagc atatgttcag tacgggcgat gatatcgtcc
    89581 tgtgcgtctg gagagagcgc ggtgaagaag gcggaatagc ccgcgacacg cactaccaga
    89641 tcgcggtact gatccggatg ttttttcgcc tccagcagcg tttcgcggga aacgatgttg
    89701 tactgaatat gccagccttt atgcacctcg aagaaggtgc gcagcaggat catcagcttc
    89761 tgcttgtcag attcgttctc cagcgttgcc ggattcagtt tctggttgag caacacgccg
    89821 ccgagaatcg ctgccgtagg cagtttaccc accgagccaa tgaccgcagt agggccgaga
    89881 tggtcagtac cagaggccgg actagcgcct tctgccagcg gggtgtgggc tttacgcccg
    89941 tccggcgttg ccatagtttg cgcgccaaac ggtacgttag cggagataga tgacgtaccc
    90001 gcgtaatagt tgccgccaac cggaccacga ccgtagcgcg gattatggta ctgtttcagt
    90061 tcgtcgatat aggtctgata agcgcgagcc agcagcgtat cgacagtatc atcgtcgttg
    90121 ccgtacttcg gcgcaccgtt aatcagccgc tgacgcagtt gctcatgagt caggccatcg
    90181 aagtcatccg ccagtgcggc agccagctgt tgctgaccaa tcgcgccctg ttcaaatacc
    90241 agtttcttca ctgccgccag gctgttgccg aggttggcaa tgccaacctg caggccagaa
    90301 acccagtcat atttcgcgcc gccttgcttg atacttttcg ctcgctcaat acagtcatcc
    90361 accagcgccg agcagagaat atcgtgcacg ttctcttcca gcatggtgtc gacgacatat
    90421 tcgatttcga ttgatttacg cgtgtaataa cggatttgcg tatcccacgc gtccattact
    90481 tcatcgaaat tgttaaagtt gcctgccgac aacgcttttt cttgtggcag gaacactttg
    90541 ccgctggtgg catcacgccc gccttccagc gccgccagca tcacgcgggc gaagttgata
    90601 aagctcatgc cggtacagcg atagccccat ttgccaccga cggcggtttc gatacaacca
    90661 atcgctgcgt agtcgtaagc gtcctgcggt tcaataccga gtttaataaa ttccgggatc
    90721 acgatttcgt cgttgttgaa cgccggcatc ccgaagccgc aacggatcac ctgtacgcag
    90781 gcgtcgagga aatcattact cattcctgca tggtaacgca cgctgaggtt aggctgagtg
    90841 gaacgcaggc gaccgcagga ttcgaggatc gcgtaagaga ggggattcac tgcatccatt
    90901 ggttgaccat caaccagatt ttgtccgcca atggtaacgt tctgatacag cggacttccc
    90961 gcagaggctt ttgagtgtga gccggagcgg atcttgttca cttccagcag tttcagccag
    91021 cagctatgca gcatctcgat ggcgtgttcg cgatccagcg tctggttgag ttcaacgtcg
    91081 cggcgatagt acgggtagag atactggtcc atacgaccaa acgataccga gtgaccgtta
    91141 gattcgatct gcaaaatcaa ttggatgaag taacacagtt gcagcgcctg ccagaaagtc
    91201 tgcggcggct ggtgggcgat aagatcgcag ttttctgcca tcgtcagcag ttcatcgcga
    91261 cggctttcgc gagtttcggt agcagccatt tcacgcgcca gggcagcgaa acgttcaatg
    91321 tgttcactga ctgccaccag cacgatatca atcgctttca ggaattgttc gccatgtaaa
    91381 tcttccagca ccgtcagatt gatgcgcgag cgacgttccg ctactttctc acgcagacca
    91441 tcaagacctt tttccagcag cagcgggaaa ttcaccgcca ggtgcgcatc gccggaggtc
    91501 atattgcctt ccgctttaat gattccggtc gccagcagac ctttttgctc atcggtaaac
    91561 atgccgtagc agcgatcctg tacggtctga ccgcgccacc acgggcacac ttcatgcaga
    91621 acgcgtttgt tctcttcgct caccgcaaag ccagcaccgg gacgatctgc cagatcatca
    91681 atctcttttt cgatccacga gacagtatat tccgggaaga tcggcgcggc gcgaacttcg
    91741 cttgcctggt tgccaatgat caactcatcg tgtttgatcc agatggtgcg attcgccagg
    91801 tgatgtgcca gcgccagcgc gcgacgtaca gggatcggct tatcgagatg ttgttgatac
    91861 atctcggtat agtgctgcgc gcgctcggta caaaccggcg gtttcacaat atgcaccagc
    91921 gcatttttgt gcgctttaat gcggtcgctg agcgtgtcca gtttcagtgt tgtcatgatt
    91981 gttatcctcg taaggtcgcg gttaaccctt tctggcaggc atactgctgg gcaaagtcga
    92041 gcagttctgg cgcatcaagc ggtttttccg gggcgtcata gggcagatta agtaagtgat
    92101 atttgttgat gcccagcgtg tggtagggca ggaaatgaat ttcgccaacg tgtagctcgt
    92161 cggcggcaaa atcggtaatg gcttttacag aggtttcgtc ggcattaaag ccctgaatca
    92221 gcggcacgcg gataatgatt tttttgcccg ctgcggcgag ttttttcagg ttatccagca
    92281 ctctggcggc gttaccgtcg gtccactgtt taaacggcgc gtcggcaacg tgttttaaat
    92341 cggcaagaaa cagatcgata tagggcagag aaggggcgat atatttccac ggcacatgca
    92401 gacaggtttc taccgcagta tgaatgcctg cctcgtggct ggcttgcagt agcgccatcg
    92461 ccatttccgg ctgcataaag ggttcaccac ccgaaagcgt taaaccgccg ccgctgcgat
    92521 cgtaaaacgg tttatcgcgc agaacggtcg tcatgatctc ctcaacgctt ttcacttcac
    92581 cacacacggt taatgcctgt gtcggacagc agtcggttaa cgccgtcaga tgctccgggg
    92641 ttaacttttc ccgatgaata agcaaaccat tcagcgcgcg ctcaatcact tccggcgcgg
    92701 ccttagcgca cagctcgcag ccttccagac acagtcgtgg gtcatacagc agatcctgcg
    92761 tgcgggcgcg gctttccggg ttctgacacc agcggcagcc cagcgaacag cctttaagaa
    92821 atacgaccgt gcggataccg gggccatcat gggtcgagta gcgctgaata ttgaaaatca
    92881 tagttgcctc tctgtttcgt tcaaacatta aaatactttc gaatgaaagt tagattgatg
    92941 tgcgtcaact gttcagagag ttttcccgtg atagtctaca ttcagacaaa aagtacattt
    93001 tgaggatggt tatggaactg tatctggata cttcagacgt tgttgcggtg aaggcgctgt
    93061 cacgtatttt tccgctggcg ggtgtgacca ctaacccaag cattatcgcc gcgggtaaaa
    93121 aaccgctgga ggttgtgctt ccggaacttc atgaagcgat gggcggtcag gggcgtctgt
    93181 ttgcccaggt aatggctacc actgctgaag ggatggtgaa tgacgcacgt aaattgcgct
    93241 ctattattgc ggatatcgtg gtgaaagttc cggtgaccgc cgaggggctg gcaactatta
    93301 agatgttaaa agcggaaggg attccgacgc tgggaaccgc ggtatatggt gcggcacaag
    93361 ggttgctgtc ggcgctggca ggtgcggaat atgttgcgcc ttacgttaat cgtattgatg
    93421 ctcagggcgg tagcggtatt cagactgtga ccgacttaca ccagttattg aaaatgcatg
    93481 cgccgcaggc gaaagtgctg gcagcgagtt tcaaaacccc gcgtcaggcg ctggactgct
    93541 tactggcagg atgtgaatca attacgctgc cgctggatgt ggcgcagcag atgattagct
    93601 atccggcggt tgatgccgct gtggcgaagt ttgagcagga ctggcaggga gcgtttggca
    93661 gaacgtcgat ttaaccggac gttctgcatc ctcataaatt gctgatgacg tggcggagtg
    93721 caccgatgcc tgccacgtct tatcaggcct ggaggtggca attactgccc gcacacctca
    93781 caccccggat tacgcatcag tttcatttcg cgaaactgac aggtcatcgc atcgtacatg
    93841 acgattttcc cgctggccgg tttgccataa cctgccagca gcttgatcgc ttccattgcc
    93901 tgcaacgaac caatcacgcc aatcaacggt gccattacgc ctgcttccac gcaggttaat
    93961 gcattttcac caaacaaacg gctgaggcag cggtagcatg gttcaccgtc ctgataagtg
    94021 aagaccgtga tttgaccttc catacgaatt gccgcgccgg aaaccagcgg taccttcgcg
    94081 gcaaaacagc ctgcgttcag ttgattacgt accgcaacgt tatccgtaca gtcgagcacc
    94141 agatcgtgtt cagcaatcat cgcagcaagt tcagcgtcat ccagcagtgc attgactggc
    94201 gtaatcgcaa tatgcgggtt gatccgcgcc agggcgtcac gtgcggattc aaccttaggt
    94261 tgcccgaccg tggcatcact gtgcagcgtc tggcgttgca gattcgagag cgaaaccgtg
    94321 tcgaagtcga gcagcgtcag gttgccgaca ccggcgcttg ccagatactg cgaggctgcg
    94381 cagccgagtc caccgaggcc gacgaccagc acacgagaat cttttagcgc ctcctgaccg
    94441 tcaaaatcaa aaccgcgcag aatgatttgc cggttgtagc gcagcatctc ctgatcgctg
    94501 agttccgcca ttacaggcct ccgaacaacg cgttaaatgg ttctacttcc acccattcgc
    94561 ccacgtccac attgccgcga tcgcgttcca gcacgataaa gcagttgccg aggctaaagg
    94621 agctaaatat atgtgaaccc tgatgtccgg tggtcgtcac ttccagttcg ccatcggcgt
    94681 tgcgttgcag cacgccgcgc tggaaatcaa ggcgacctgg cgtttttttc aggcgggacg
    94741 ctgtgcgtac gcgctggcgc gcgggcaggc cgctggcggt gttaccgctt agttttgcca
    94801 gcaaaggctg taccagttga tagaaggtca gcgtcgctga aaccgggttg cccggcaggc
    94861 cgcagaacca gctattagaa agtttaccga acgcgaacgg tttacccggt ttaatcgcca
    94921 gcttccagaa ggcgatctcc cccagctctt caagaatggt tttggtgtaa tccgcttcac
    94981 ccactgaaac accgccggaa ctgatcacca catccgcctg gctgtcggct tcaataaatg
    95041 cggcgcgcag ggcatgtgga tcgtcgcgga taatccctaa gttgattacc tcgcatccca
    95101 actgttctaa catcaggtgt acggcgagac ggttggtatc gtagatttgg ccatcgccca
    95161 gtggctgacc gggcaactgg agttcatcac cggtagaaaa aagcgctaca cgcactttac
    95221 taatcaccgg aacttcggca atccccagtg aagcaatcac tggcagttca gcggtagtca
    95281 ggcgagttcc cgccgggaaa acaaccgcac ctgcagagat atcttcaccg cgacggcgaa
    95341 tattttgccc gctacgcact tcagcagtaa aacgcacgcc attgtccgtt tgttcagtct
    95401 gctcctgcat caccaccgct tcgcagcctt ccggcaccgg cgcaccggtc ataatacgaa
    95461 tgcaggtacc cgcaggccat tcaccatggt acggctgacc ggcaaaggat ttaccggcaa
    95521 cgggcagcgg ttgcctggag gcaatatcgg ctaaacgcac cgcgtagccg tccattgcgg
    95581 agttatcaaa ccccggaaca tcaagcggcg aaacgatatc gctcgccaga atacgaccaa
    95641 aacactgtac cagtggcagc gtttcctggg cggtcagtgg ggtgacgcga gaaagcatct
    95701 cattaagcgc ggtgtcgagc gacatcaatc cggtggtaaa ttccatgaaa acactcctgc
    95761 ggaggcaaaa tcgaatttgc ctattatgtc agaaaaacgc cacagactgt atgccacctc
    95821 gggcgtagcg ttggctcctg
//
LOCUS       DAZFFI010000006        86463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFFI010000006 DAZFFI010000000
VERSION     DAZFFI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.006"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1134
                     /gene="proV"
                     /locus_tag="ACHJ12_000549"
     CDS             <1..1134
                     /gene="proV"
                     /locus_tag="ACHJ12_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4988389.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1127..2191
                     /gene="proW"
                     /locus_tag="ACHJ12_000550"
     CDS             1127..2191
                     /gene="proW"
                     /locus_tag="ACHJ12_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4988390.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2248..3240
                     /gene="proX"
                     /locus_tag="ACHJ12_000551"
     CDS             2248..3240
                     /gene="proX"
                     /locus_tag="ACHJ12_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4988391.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3531..4715
                     /locus_tag="ACHJ12_000552"
     CDS             3531..4715
                     /locus_tag="ACHJ12_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988392.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4839..5576
                     /gene="ygaZ"
                     /locus_tag="ACHJ12_000553"
     CDS             4839..5576
                     /gene="ygaZ"
                     /locus_tag="ACHJ12_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4988393.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5566..5901
                     /gene="ygaH"
                     /locus_tag="ACHJ12_000554"
     CDS             5566..5901
                     /gene="ygaH"
                     /locus_tag="ACHJ12_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4988394.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5992..6522
                     /gene="emrR"
                     /locus_tag="ACHJ12_000555"
     CDS             5992..6522
                     /gene="emrR"
                     /locus_tag="ACHJ12_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4988395.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6649..7821
                     /gene="emrA"
                     /locus_tag="ACHJ12_000556"
     CDS             6649..7821
                     /gene="emrA"
                     /locus_tag="ACHJ12_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4988396.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7838..9376
                     /gene="emrB"
                     /locus_tag="ACHJ12_000557"
     CDS             7838..9376
                     /gene="emrB"
                     /locus_tag="ACHJ12_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4988397.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9440..9955)
                     /gene="luxS"
                     /locus_tag="ACHJ12_000558"
     CDS             complement(9440..9955)
                     /gene="luxS"
                     /locus_tag="ACHJ12_000558"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4988398.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(10105..11661)
                     /gene="gshA"
                     /locus_tag="ACHJ12_000559"
     CDS             complement(10105..11661)
                     /gene="gshA"
                     /locus_tag="ACHJ12_000559"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4988399.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11734..12162)
                     /gene="yqaA"
                     /locus_tag="ACHJ12_000560"
     CDS             complement(11734..12162)
                     /gene="yqaA"
                     /locus_tag="ACHJ12_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4988400.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12159..12725)
                     /gene="yqaB"
                     /locus_tag="ACHJ12_000561"
     CDS             complement(12159..12725)
                     /gene="yqaB"
                     /locus_tag="ACHJ12_000561"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4988401.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13006..13082)
                     /locus_tag="ACHJ12_000562"
     tRNA            complement(13006..13082)
                     /locus_tag="ACHJ12_000562"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13046..13048),aa:Arg,seq:acg)
     gene            complement(13281..13357)
                     /locus_tag="ACHJ12_000563"
     tRNA            complement(13281..13357)
                     /locus_tag="ACHJ12_000563"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13321..13323),aa:Arg,seq:acg)
     gene            complement(13421..13497)
                     /locus_tag="ACHJ12_000564"
     tRNA            complement(13421..13497)
                     /locus_tag="ACHJ12_000564"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13461..13463),aa:Arg,seq:acg)
     gene            complement(13695..13771)
                     /locus_tag="ACHJ12_000565"
     tRNA            complement(13695..13771)
                     /locus_tag="ACHJ12_000565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13735..13737),aa:Arg,seq:acg)
     gene            complement(13775..13867)
                     /locus_tag="ACHJ12_000566"
     tRNA            complement(13775..13867)
                     /locus_tag="ACHJ12_000566"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13831..13833),aa:Ser,seq:gct)
     gene            complement(14183..14368)
                     /gene="csrA"
                     /locus_tag="ACHJ12_000567"
     CDS             complement(14183..14368)
                     /gene="csrA"
                     /locus_tag="ACHJ12_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4988402.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14603..17233)
                     /gene="alaS"
                     /locus_tag="ACHJ12_000568"
     CDS             complement(14603..17233)
                     /gene="alaS"
                     /locus_tag="ACHJ12_000568"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4988403.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17361..17861)
                     /gene="recX"
                     /locus_tag="ACHJ12_000569"
     CDS             complement(17361..17861)
                     /gene="recX"
                     /locus_tag="ACHJ12_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4988404.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18104..19165)
                     /gene="recA"
                     /locus_tag="ACHJ12_000570"
     CDS             complement(18104..19165)
                     /gene="recA"
                     /locus_tag="ACHJ12_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4988405.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19245..19742)
                     /gene="pncC"
                     /locus_tag="ACHJ12_000571"
     CDS             complement(19245..19742)
                     /gene="pncC"
                     /locus_tag="ACHJ12_000571"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4988406.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19887..20972)
                     /gene="mltB"
                     /locus_tag="ACHJ12_000572"
     CDS             complement(19887..20972)
                     /gene="mltB"
                     /locus_tag="ACHJ12_000572"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4988407.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21228..21791
                     /gene="srlA"
                     /locus_tag="ACHJ12_000573"
                     /gene_synonym="gutA"
     CDS             21228..21791
                     /gene="srlA"
                     /locus_tag="ACHJ12_000573"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO4988408.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21788..22747
                     /gene="srlE"
                     /locus_tag="ACHJ12_000574"
                     /gene_synonym="gutE"
     CDS             21788..22747
                     /gene="srlE"
                     /locus_tag="ACHJ12_000574"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO4988409.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22758..23129
                     /gene="srlB"
                     /locus_tag="ACHJ12_000575"
                     /gene_synonym="gutB"
     CDS             22758..23129
                     /gene="srlB"
                     /locus_tag="ACHJ12_000575"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4988410.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23133..23912
                     /gene="srlD"
                     /locus_tag="ACHJ12_000576"
     CDS             23133..23912
                     /gene="srlD"
                     /locus_tag="ACHJ12_000576"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4988411.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24017..24376
                     /gene="gutM"
                     /locus_tag="ACHJ12_000577"
     CDS             24017..24376
                     /gene="gutM"
                     /locus_tag="ACHJ12_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4988412.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24443..25216
                     /gene="srlR"
                     /locus_tag="ACHJ12_000578"
                     /gene_synonym="gutR"
     CDS             24443..25216
                     /gene="srlR"
                     /locus_tag="ACHJ12_000578"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4988413.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25209..26174
                     /gene="gutQ"
                     /locus_tag="ACHJ12_000579"
     CDS             25209..26174
                     /gene="gutQ"
                     /locus_tag="ACHJ12_000579"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4988414.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26171..27685)
                     /gene="norR"
                     /locus_tag="ACHJ12_000580"
     CDS             complement(26171..27685)
                     /gene="norR"
                     /locus_tag="ACHJ12_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4988415.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27872..29311
                     /gene="norV"
                     /locus_tag="ACHJ12_000581"
     CDS             27872..29311
                     /gene="norV"
                     /locus_tag="ACHJ12_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4988416.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29308..30441
                     /gene="norW"
                     /locus_tag="ACHJ12_000582"
     CDS             29308..30441
                     /gene="norW"
                     /locus_tag="ACHJ12_000582"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4988417.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30569..32821)
                     /gene="hypF"
                     /locus_tag="ACHJ12_000583"
     CDS             complement(30569..32821)
                     /gene="hypF"
                     /locus_tag="ACHJ12_000583"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4988418.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGAMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32974..33501)
                     /gene="hydN"
                     /locus_tag="ACHJ12_000584"
     CDS             complement(32974..33501)
                     /gene="hydN"
                     /locus_tag="ACHJ12_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4988419.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33650..34660)
                     /gene="ascG"
                     /locus_tag="ACHJ12_000585"
     CDS             complement(33650..34660)
                     /gene="ascG"
                     /locus_tag="ACHJ12_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4988420.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            34920..36377
                     /gene="ascF"
                     /locus_tag="ACHJ12_000586"
     CDS             34920..36377
                     /gene="ascF"
                     /locus_tag="ACHJ12_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO4988421.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            36386..37810
                     /gene="ascB"
                     /locus_tag="ACHJ12_000587"
     CDS             36386..37810
                     /gene="ascB"
                     /locus_tag="ACHJ12_000587"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4988422.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37924..38343)
                     /locus_tag="ACHJ12_000588"
     CDS             complement(37924..38343)
                     /locus_tag="ACHJ12_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4988423.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38414..39169)
                     /locus_tag="ACHJ12_000589"
     CDS             complement(38414..39169)
                     /locus_tag="ACHJ12_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988424.1"
                     /translation="MCAFLLSLVLPAQATSFTDYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(39212..39682)
                     /gene="hycI"
                     /locus_tag="ACHJ12_000590"
     CDS             complement(39212..39682)
                     /gene="hycI"
                     /locus_tag="ACHJ12_000590"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4988425.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(39675..40085)
                     /gene="hycH"
                     /locus_tag="ACHJ12_000591"
     CDS             complement(39675..40085)
                     /gene="hycH"
                     /locus_tag="ACHJ12_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4988426.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40082..40849)
                     /gene="hycG"
                     /locus_tag="ACHJ12_000592"
     CDS             complement(40082..40849)
                     /gene="hycG"
                     /locus_tag="ACHJ12_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4988427.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(40849..41391)
                     /gene="hycF"
                     /locus_tag="ACHJ12_000593"
     CDS             complement(40849..41391)
                     /gene="hycF"
                     /locus_tag="ACHJ12_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4988428.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41401..43110)
                     /gene="hycE"
                     /locus_tag="ACHJ12_000594"
     CDS             complement(41401..43110)
                     /gene="hycE"
                     /locus_tag="ACHJ12_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4988429.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43128..44051)
                     /gene="hycD"
                     /locus_tag="ACHJ12_000595"
     CDS             complement(43128..44051)
                     /gene="hycD"
                     /locus_tag="ACHJ12_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4988430.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44054..45880)
                     /gene="hycC"
                     /locus_tag="ACHJ12_000596"
     CDS             complement(44054..45880)
                     /gene="hycC"
                     /locus_tag="ACHJ12_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4988431.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(45877..46488)
                     /gene="hycB"
                     /locus_tag="ACHJ12_000597"
     CDS             complement(45877..46488)
                     /gene="hycB"
                     /locus_tag="ACHJ12_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4988432.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46613..47074)
                     /gene="hycA"
                     /locus_tag="ACHJ12_000598"
     CDS             complement(46613..47074)
                     /gene="hycA"
                     /locus_tag="ACHJ12_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4988433.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47286..47636
                     /gene="hypA"
                     /locus_tag="ACHJ12_000599"
     CDS             47286..47636
                     /gene="hypA"
                     /locus_tag="ACHJ12_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4988434.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47640..48512
                     /gene="hypB"
                     /locus_tag="ACHJ12_000600"
     CDS             47640..48512
                     /gene="hypB"
                     /locus_tag="ACHJ12_000600"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4988435.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48503..48775
                     /gene="hypC"
                     /locus_tag="ACHJ12_000601"
     CDS             48503..48775
                     /gene="hypC"
                     /locus_tag="ACHJ12_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4988436.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48775..49896
                     /gene="hypD"
                     /locus_tag="ACHJ12_000602"
     CDS             48775..49896
                     /gene="hypD"
                     /locus_tag="ACHJ12_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4988437.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            49893..50903
                     /gene="hypE"
                     /locus_tag="ACHJ12_000603"
     CDS             49893..50903
                     /gene="hypE"
                     /locus_tag="ACHJ12_000603"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4988438.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDMLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            50977..53055
                     /gene="flhA"
                     /locus_tag="ACHJ12_000604"
     CDS             50977..53055
                     /gene="flhA"
                     /locus_tag="ACHJ12_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4988439.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53092..53445)
                     /gene="ygbA"
                     /locus_tag="ACHJ12_000605"
     CDS             complement(53092..53445)
                     /gene="ygbA"
                     /locus_tag="ACHJ12_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4988440.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(53477..53656)
                     /locus_tag="ACHJ12_000606"
     CDS             complement(53477..53656)
                     /locus_tag="ACHJ12_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988441.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            53732..56293
                     /gene="mutS"
                     /locus_tag="ACHJ12_000607"
     CDS             53732..56293
                     /gene="mutS"
                     /locus_tag="ACHJ12_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4988442.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELER
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            56399..57055
                     /gene="pphB"
                     /locus_tag="ACHJ12_000608"
     CDS             56399..57055
                     /gene="pphB"
                     /locus_tag="ACHJ12_000608"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4988443.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(57106..57873)
                     /gene="ygbI"
                     /locus_tag="ACHJ12_000609"
     CDS             complement(57106..57873)
                     /gene="ygbI"
                     /locus_tag="ACHJ12_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO4988444.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            58069..58977
                     /gene="ltnD"
                     /locus_tag="ACHJ12_000610"
     CDS             58069..58977
                     /gene="ltnD"
                     /locus_tag="ACHJ12_000610"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO4988445.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            58974..60236
                     /gene="otnK"
                     /locus_tag="ACHJ12_000611"
     CDS             58974..60236
                     /gene="otnK"
                     /locus_tag="ACHJ12_000611"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO4988446.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            60233..60871
                     /gene="ygbL"
                     /locus_tag="ACHJ12_000612"
     CDS             60233..60871
                     /gene="ygbL"
                     /locus_tag="ACHJ12_000612"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO4988447.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            60876..61652
                     /gene="ygbM"
                     /locus_tag="ACHJ12_000613"
     CDS             60876..61652
                     /gene="ygbM"
                     /locus_tag="ACHJ12_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4988448.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            61741..63105
                     /gene="ygbN"
                     /locus_tag="ACHJ12_000614"
     CDS             61741..63105
                     /gene="ygbN"
                     /locus_tag="ACHJ12_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO4988449.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPQLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(63144..63380)
                     /locus_tag="ACHJ12_000615"
     CDS             complement(63144..63380)
                     /locus_tag="ACHJ12_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4988450.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(63391..64818)
                     /locus_tag="ACHJ12_000616"
     CDS             complement(63391..64818)
                     /locus_tag="ACHJ12_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4988451.1"
                     /translation="MAFDDLRSFLQELDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(64818..65411)
                     /locus_tag="ACHJ12_000617"
     CDS             complement(64818..65411)
                     /locus_tag="ACHJ12_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4988452.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            65558..65965
                     /locus_tag="ACHJ12_000618"
     CDS             65558..65965
                     /locus_tag="ACHJ12_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4988453.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(66086..67078)
                     /gene="rpoS"
                     /locus_tag="ACHJ12_000619"
     CDS             complement(66086..67078)
                     /gene="rpoS"
                     /locus_tag="ACHJ12_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4988454.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67141..68235)
                     /gene="nlpD"
                     /locus_tag="ACHJ12_000620"
     CDS             complement(67141..68235)
                     /gene="nlpD"
                     /locus_tag="ACHJ12_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4988455.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVKQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68420..69046)
                     /gene="pcm"
                     /locus_tag="ACHJ12_000621"
     CDS             complement(68420..69046)
                     /gene="pcm"
                     /locus_tag="ACHJ12_000621"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4988456.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69040..69801)
                     /gene="surE"
                     /locus_tag="ACHJ12_000622"
     CDS             complement(69040..69801)
                     /gene="surE"
                     /locus_tag="ACHJ12_000622"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4988457.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(69782..70831)
                     /gene="truD"
                     /locus_tag="ACHJ12_000623"
     CDS             complement(69782..70831)
                     /gene="truD"
                     /locus_tag="ACHJ12_000623"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4988458.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(70828..71307)
                     /gene="ispF"
                     /locus_tag="ACHJ12_000624"
     CDS             complement(70828..71307)
                     /gene="ispF"
                     /locus_tag="ACHJ12_000624"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4988459.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71307..72017)
                     /gene="ispD"
                     /locus_tag="ACHJ12_000625"
     CDS             complement(71307..72017)
                     /gene="ispD"
                     /locus_tag="ACHJ12_000625"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4988460.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72036..72347)
                     /gene="ftsB"
                     /locus_tag="ACHJ12_000626"
     CDS             complement(72036..72347)
                     /gene="ftsB"
                     /locus_tag="ACHJ12_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4988461.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72541..72864)
                     /gene="ygbE"
                     /locus_tag="ACHJ12_000627"
     CDS             complement(72541..72864)
                     /gene="ygbE"
                     /locus_tag="ACHJ12_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4988462.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(72914..73519)
                     /gene="cysC"
                     /locus_tag="ACHJ12_000628"
     CDS             complement(72914..73519)
                     /gene="cysC"
                     /locus_tag="ACHJ12_000628"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4988463.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73519..74946)
                     /gene="cysN"
                     /locus_tag="ACHJ12_000629"
     CDS             complement(73519..74946)
                     /gene="cysN"
                     /locus_tag="ACHJ12_000629"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4988464.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(74948..75856)
                     /gene="cysD"
                     /locus_tag="ACHJ12_000630"
     CDS             complement(74948..75856)
                     /gene="cysD"
                     /locus_tag="ACHJ12_000630"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4988465.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76108..77145
                     /gene="iap"
                     /locus_tag="ACHJ12_000631"
     CDS             76108..77145
                     /gene="iap"
                     /locus_tag="ACHJ12_000631"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4988466.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77227..77804
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77227..77255
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(77901..78194)
                     /gene="cas2e"
                     /locus_tag="ACHJ12_000632"
     CDS             complement(77901..78194)
                     /gene="cas2e"
                     /locus_tag="ACHJ12_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4988467.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGKNRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(78194..79114)
                     /gene="cas1e"
                     /locus_tag="ACHJ12_000633"
     CDS             complement(78194..79114)
                     /gene="cas1e"
                     /locus_tag="ACHJ12_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4988468.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLINKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(79111..79761)
                     /gene="cas6e"
                     /locus_tag="ACHJ12_000634"
     CDS             complement(79111..79761)
                     /gene="cas6e"
                     /locus_tag="ACHJ12_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4988469.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRPFVSELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVKGQAEGRDIWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENTRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(79743..80489)
                     /gene="cas5e"
                     /locus_tag="ACHJ12_000635"
     CDS             complement(79743..80489)
                     /gene="cas5e"
                     /locus_tag="ACHJ12_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4988470.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDTLRNAYQQYQDHFRKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(80500..81555)
                     /gene="cas7e"
                     /locus_tag="ACHJ12_000636"
     CDS             complement(80500..81555)
                     /gene="cas7e"
                     /locus_tag="ACHJ12_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4988471.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTDYASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(81567..82106)
                     /gene="casB"
                     /locus_tag="ACHJ12_000637"
                     /gene_synonym="cse2"
     CDS             complement(81567..82106)
                     /gene="casB"
                     /locus_tag="ACHJ12_000637"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4988472.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(82103..83665)
                     /gene="casA"
                     /locus_tag="ACHJ12_000638"
                     /gene_synonym="cse1"
     CDS             complement(82103..83665)
                     /gene="casA"
                     /locus_tag="ACHJ12_000638"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4988473.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(83763..86462)
                     /gene="cas3"
                     /locus_tag="ACHJ12_000639"
     CDS             complement(83763..86462)
                     /gene="cas3"
                     /locus_tag="ACHJ12_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO4988474.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQKALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        20264 a        23554 c        22233 g        20412 t
ORIGIN      
        1 tatatcgaac aaggactttc gaaagaacaa attctggaaa aaactgggct atcgcttggc
       61 gtaaaagacg ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc
      121 ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg
      181 caagtgctga ttgatggtgt ggatattgcc aaaatatccg atgccgaact ccgtgaggtg
      241 cgcagaaaaa agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg
      301 ctggacaata ctgcgttcgg tatggaattg gccggaatta atgccgaaga acgccgggaa
      361 aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccggat
      421 gaactctctg gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg
      481 gatatattat taatggacga agccttctcg gcgctcgatc cattaattcg caccgagatg
      541 caggatgagc tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac
      601 gatctcgatg aggctatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg
      661 gtacaggtcg gcacaccgga tgaaattctc aataatccgg cgaatgatta tgtccgtacc
      721 ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga aagatattgc ccgccggaca
      781 ccgaatggct taattcgtaa aacccctggc ttcggcccac gttcggcact gaaattattg
      841 caggatgaag atcgcgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca
      901 gtttccatcg attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg
      961 attgatgcgc cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc
     1021 ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt
     1081 tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa
     1141 taatccgtgg gataccacgc cagcggcgga cagtgccgtg caatccgcag acgcttgggg
     1201 tacaccggcg actgcaccga ctgacggcgg tggtgctgac tggctgacca gtacgcctgc
     1261 gccaaacgtc gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga
     1321 cagttgggtc actgaaggga tcgactgggt tgttacccat ttccgtcccg tcttccaggg
     1381 cgtgcgcgtt ccggttgatt acattctcaa cggtttccag caattgctgc tgggtatgcc
     1441 cgcaccggtg gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat
     1501 gggcgtggcg acgctggttt cgctgattgc catcggcgca atcggtgcct ggtcgcaggc
     1561 catggttacc ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca ttggtttgcc
     1621 gctggggatc tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga
     1681 tgccatgcag accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat
     1741 cggtaacgtg ccgggcgtgg tggtgacgat catctttgct ctgccgccga ttatccgtct
     1801 gaccattctg gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg
     1861 tgccagcccg cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat
     1921 ggcgggcgtt aaccagacgc tgatgctggc cctttctatg gtggtcattg cctcgatgat
     1981 tgccgtcggc gggctgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct
     2041 tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt atcctcgacc gcctgacgca
     2101 ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt
     2161 cggtctgctg acccgcccat tcattaagta actctgcact tgcccggtaa cgccgggcat
     2221 atcaccctgc caaaaaaagg aataacaatg cgacatagcg tactttttgc gacagcgttt
     2281 gccacgctta tctctacaca aacttttgct gccgacctgc cgggcaaagg cattacagtt
     2341 aatccagttc aaagcaccat caccgaagaa accttccaga cactgctggt cagtcgtgcg
     2401 ctggagaaat taggttatac cgtcaataaa cccagcgaag tagattacaa cgttggctac
     2461 acctcgcttg cttccggcga tgcaaccttc accgccgtga actggacgcc gctgcatgac
     2521 aacatgtacg aagctgccgg tggcgataag aaattttatc gtgaaggggt atttgttaac
     2581 ggcgcggcac agggttacct gatcgataag aaaaccgccg accagtataa aatcaccaac
     2641 atcgcacaac tgaaagatcc gaagatcgcc aaactgttcg ataccaacgg cgacggtaaa
     2701 gcggatttaa ccggttgtaa ccctggctgg ggctgcgaag gtgccatcaa ccaccagctt
     2761 gccgcgtatg aactgaccaa caccgtgacg cataatcagg ggaactacgc agcgatgatg
     2821 gccgacacca tcagtcgcta caaagagggc aaaccggtgt tttattacac ctggacgccg
     2881 tactgggtga gtaatgagct gaagccaggg aaagatgtgg tctggttgca ggtgccgttc
     2941 tccgcactgc cgggcgataa aaatgccgat accaaactgc cgaatggcgc gaattacggc
     3001 ttcccggtca gcaccatgca tatcgttgcc aacaaagcct ggaccgagaa aaatccggcg
     3061 gcagccaaac tgtttgccat tatgcagttg ccagtggccg atattaacgc ccagaacgcc
     3121 attatgcatg acggcaaagc ctcagaaggc gatattcagg gccacgttga tggctggatc
     3181 aaagcccacc agcagcagtt cgatggctgg gtgaatgagg cgctggcagc gcagaagtaa
     3241 tttttattca ggcggataag gcgtttttgc ctcatccgcc gctctgtaca caatgcctga
     3301 tgcgacgctg acgcgtctta tcatgcctac aaaatacact cattcgtatg gcggataagg
     3361 cgttttcgcc gcatccgccg ttctgtgcac aatgcctgat gcgacgctgg cgcgtcttat
     3421 catgcctaca aaccacatcg cacatttcaa caatctatct ttcatcccat attcatcaac
     3481 atccgctatt attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac
     3541 ctaatcatga gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg
     3601 tagccagtaa ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt
     3661 ccgccagttc ggcaggcttt attgttaccg ccgcgcagtt gggctacgcc gcaggtctac
     3721 tgtttcttgt tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct
     3781 tactggcagc gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc
     3841 tcggtacggc attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag
     3901 cgacgctggc ttcaccggac aaacgcggca aagtggttgg cactattatg agcggtctgc
     3961 tgttggggat cttactcgca cggaccgttg ccggattgct ggcgaatctc ggcggctggc
     4021 gcaccgtctt ttgggttgcc tcggtgttaa tggcactgat ggcgctggca ttatggcgtg
     4081 gtctgccaca aatgaaatca gaaacccacc tcaactaccc acagttgtta ggttccgttt
     4141 tcagcatgtt tatcagcgat aagatcctgc gcacccgcgc attgctgggc tgcctgacct
     4201 ttgccaactt cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta
     4261 actacagcga tggtgtcatt ggtctgtttg gacttgcggg agctgccgga gcgttgggcg
     4321 ctcgtccggc gggcggtttt gccgataagg gtaaatcgca ccacaccaca actttcggtc
     4381 tgctgctgct attactttca tggctggcga tctggtttgg tcacacttcc gtactggcgc
     4441 tgattatcgg catcctggtg ctggatctca ccgtacaggg cgtgcatatc actaaccaga
     4501 cggtaattta tcgaatacac cctgatgcgc gtaatcgcct gaccgcaggt tacatgacca
     4561 gctactttat tggcggtgcc gccggttcgc taatttcagc ctcagcctgg caacatggcg
     4621 gttgggctgg cgtttgtctg gctggcgcga cgattgccct ggttaactta ctggtctggt
     4681 ggcgaggttt tcatcgtcag gaagccgcaa attaagcaat cagcaaccgt tttcgtggct
     4741 ttacacttat aagggtgtta agaagcccat cagtctgata aggttaagat attcattcag
     4801 tctatttata atattaacaa tcgttaagcg tacactctat ggaaagccct actccacagc
     4861 ctgctcctgg ttcggcgacc ttcatggaag gatgcaaaga cagtttaccg attgttatta
     4921 gttatattcc ggtggccttt gcgttcggtc tgaatgcgac ccgtctggga ttctctcctc
     4981 tcgaaagcgt ttttttctcc tgcatcattt atgcaggcgc gagccagttc gtcattaccg
     5041 cgatgctggc agccgggagt agtttgtggg ttgctgcact gaccgtcatg gcaatggatg
     5101 ttcgccatgt gttgtatggc ccgtcactgc gtagccgtat tattcagcgt ctgcaaaaat
     5161 cgaaaaccgc cctgtgggca tttggcctga cggatgaggt ttttgccgcc gcaaccgcaa
     5221 aactggtacg caataatcgc cgctggagcg agaactggat gatcggcatt gccttcagtt
     5281 catggtcatc gtgggtattt ggtacggtaa taggggcatt ctccggcagc ggcttgctgc
     5341 aaggttatcc cgccgttgaa gctgcattag gttttatgct tccggcactc tttatgagtt
     5401 tcctgctcgc ctctttccag cgcaaacaat ctctttgcgt taccgcagcg ttagttggtg
     5461 cccttgcagg cgtaacgcta ttttctattc ccgtcgccat tctggcaggc attgtctgtg
     5521 gctgcctcac tgcgttaatc caggcattct ggcaaggagc gcccgatgag ctatgaggtt
     5581 ctgctgcttg ggttactggt tggcgtggcg aattattgct tccgctattt gccgctgcgc
     5641 ctgcgtgtgg gtaatgcccg cccaaccaaa cgtggcgcgg taggtatttt gctcgacacc
     5701 attggcatcg cctcgatatg cgctctgctg gttgtctcta ccgcaccaga agtgatgcac
     5761 gatacacgcc gtttcgtgcc cacgctggtc ggcttcgcgg tactgggtgc cagtttctat
     5821 aaaacacgca gcattatcat cccaacactg cttagtgcgc tggcctatgg gctcgcctgg
     5881 aaagtgatgg cgattatata acccacaaga atcatttttc taaaacaata catttacttt
     5941 atttgtcact gtcgttacta tatcggctga aattaatgag gtcataccca aatggatagt
     6001 tcgtttacgc ccattgaaca aatgctaaaa tttcgcgcca gccgccacga agattttcct
     6061 tatcaggaga tccttctgac tcgtctttgc atgcacatgc aaagcaagct gctggagaac
     6121 cgcaataaaa tgctgaaggc tcaggggatt aacgagacgt tgtttatggc gttgattacg
     6181 ctggagtctc aggaaaacca cagtattcag ccttctgaat taagttgtgc tcttggatca
     6241 tcccgtacca acgcgacgcg tattgccgat gaactggaaa aacgcggttg gatcgaacgt
     6301 cgtgaaagcg ataacgatcg ccgctgcctg catctgcaat taacggaaaa aggtcacgag
     6361 tttttgcgcg aggttttacc accgcagcat aactgcctgc atcaactctg gtccgcgctc
     6421 agcacaacag aaaaagatca gctcgagcaa atcacccgca aattgctctc ccgtctcgac
     6481 cagatggaac aagacggtgt ggttctcgaa gcgatgagct aacgcgtcat ctcgctcaaa
     6541 aatccagatt tataaaagaa aaaatgaccg gccagcatcg caacatgctg gcctttttgg
     6601 caagcaggtc ggctcagccg atgagttaag aagatcgtgg agaacaatat gagcgcaaat
     6661 gcggagactc aaaccccgca gcaaccggta aagaagagcg gcaaacgtaa gcgtctgctc
     6721 ctccttctca ccttgctctt tataattatt gccgtagcga tagggattta ttggtttttg
     6781 gtactgcgtc atttcgaaga aaccgatgac gcatacgtgg cagggaatca gattcaaatt
     6841 atgtctcagg tgtctggcag cgtgacgaaa gtctgggccg ataacaccga ttttgtaaaa
     6901 gaaggcgatg ttctggtcac tctcgacccg acagatgctc gccaggcgtt tgaaaaagcc
     6961 aaaactgcac tggcttccag cgttcgccaa acccaccagc tgatgattaa cagcaaacag
     7021 ttgcaggcga atattgaggt gcaaaaaatc gccctcgcga aagcacaaag cgactacaac
     7081 cgccgtgtgc cgctgggcaa tgccaacctg attggtcgcg aagagctgca acacgcccgc
     7141 gacgccgtca ccagtgccca ggcgcaactg gacgtcgcta ttcaacaata caatgccaat
     7201 caggcgatga ttctggggac taaactggaa gatcagcctg ccgtgcaaca ggctgccacc
     7261 gaagtacgta acgcctggct ggcgctggag cgtacccgta ttgtcagtcc gatgaccggt
     7321 tatgtctccc gccgcgcggt acagcctggg gcgcaaatta gcccaacgac gccgctgatg
     7381 gcggtcgttc cagccaccaa tatgtgggtg gatgccaact ttaaagagac gcagattgcc
     7441 aatatgcgta tcggtcagcc ggtcaccatc accaccgata tttacggcga tgatgtgaaa
     7501 tacaccggta aagtggttgg tctggatatg ggcacaggta gcgcgttctc actgcttccg
     7561 gcgcaaaatg cgaccggtaa ctggatcaaa gtcgttcagc gtctacctgt gcgtatcgaa
     7621 ctggaccaga aacagctgga gcaatacccg ctgcgcattg gtttgtccac gctggtgagc
     7681 gtcaatacca ctaaccgtga cggtcaggta ctggcaaata aagtacgttc aacaccggtc
     7741 gcggtaagta ccgcgcgtga aatcagcctg gcacctgtca ataaactgat cgacgatatc
     7801 gtaaaagcta acgctggcta atccagaggt gcgtgtgatg caacagcaaa aaccgctgga
     7861 aggcgcgcaa ctggtcatta tgacgattgc gctgtcactg gcgacattca tgcaggtgct
     7921 ggactccacc attgctaacg tggcgatccc cactatcgcc gggaatctgg gctcatcgct
     7981 cagccaggga acgtgggtaa tcacttcttt cggggtggcg aatgccatct cgatcccgct
     8041 taccggctgg ctggcaaagc gcgtcgggga agtgaaactg ttcctttggt ccaccatcgc
     8101 ctttgctatt gcgtcgtggg cgtgtggtgt ctccagcagc ctgaatatgc tgatcttctt
     8161 ccgcgtgatt caggggattg tcgccgggcc gttgatcccg ctttcgcaaa gtctattgct
     8221 gaataactac ccgccagcca aacgctcgat cgcgctggcg ttgtggtcga tgacggtgat
     8281 tgtcgcgcca atttgcggcc cgatcctcgg cggttatatc agcgataatt accactgggg
     8341 ctggatattc ttcatcaacg tgccgattgg cgtggcggtg gtgttgatga cactgcaaac
     8401 tctgcgcgga cgtgaaaccc gcaccgaacg gcggcggatt gatgccgtgg ggctggcact
     8461 gctggttatt ggtatcggca gcctgcagat tatgctcgac cgcggtaaag agctggactg
     8521 gttctcatca caggaaatta tcattcttac cgtggtggca gtggtggcta tctgcttcct
     8581 aattgtctgg gagttgaccg acgataaccc gatagtcgat ctgtcgttgt ttaagtcgcg
     8641 caacttcacc atcggctgct tgtgtatcag cctcgcgtat atgctctact tcggcgcgat
     8701 tgttctgctg ccgcagttgt tgcaggaggt ctacggttac acggcaacgt gggcaggtct
     8761 ggcctctgcg ccggtaggga ttattccggt gatcctgtcg ccgattatcg gccgcttcgc
     8821 gcataaactg gatatgcggc ggctggtaac cttcagcttt attatgtatg ccgtctgctt
     8881 ctactggcgt gcctatacct ttgaaccagg tatggatttt ggcgcgtcgg cctggccgca
     8941 gtttatccag gggtttgcgg tggcctgctt ctttatgccg ctgaccacca ttacgctgtc
     9001 tggtttgcca ccggaacgac tggcggcggc atcgagcctc tccaacttta cgcgtacgct
     9061 ggcggggtct atcggcacgt ccatcaccac caccatgtgg accaaccgcg agtcgatgca
     9121 ccacgcacag ttaaccgagt cggtaaaccc gtttaaccca aacgcccagg cgatgtacag
     9181 tcaactggaa gggcttggga tgacgcaaca gcaggcctcc ggctggattg cccagcagat
     9241 caccaaccag gggctgatta tttccgccaa tgagatcttc tggatgtcag ccgggatatt
     9301 cctcgtcctg ctggggctgg tgtggtttgc taaaccgcca tttggcgcag gtggcggcgg
     9361 aggcggtgcg cactaagtac aactaagcca gttcatttga actggctttt ttcaattaat
     9421 tgtgaagata gtttactgac taaatgtgca gttcctgcaa cttctcttcc ggcagcgcca
     9481 gttcttcgtt gctgttgatg cgcacgtcac gttccagaat gctacgcgca atatcctgcg
     9541 cttcctgcaa cgagtgcatc tggtaagtgc cacactggta gacgttcagt tccgggatct
     9601 gattctgatc ctgcactttc agcacgtctt ccattgccgc tttccaggca tcagcaacac
     9661 gctgctcatc tggcgtacca atcagactca tataaaaacc ggtgcggcag cccattggcg
     9721 agatatcgat aatctctaca ccattaccgt taagatggtt acgcataaaa ccagcaaaca
     9781 ggtgctccag ggtatggatc cctctttctg gcatcacttc tttgttcggc acgcagaagc
     9841 gcagatcgaa cacggtgatt gcgtcgccat gcggggtgtt cattgttttc gccacccgaa
     9901 ctgcaggcgc ttccatccgg gtatgatcga ctgtgaagct atctaacaac ggcatttagc
     9961 cacctccggt aattttttta aaaattttct gaactctttc ttcccaggcg agtctgagta
    10021 tatgaaagac gcgcatttgt tatcatcatc cctgaattca gagatgaaat tttggccact
    10081 cacgagtggc ctttttcttt tctgtcaggc gtgtttttcc agccacaccg caaacggttc
    10141 ggtatcagcg gcttccattt cctgctgacg gcgttcagac gcctcgcgct cggctacaaa
    10201 atcctcttcg cgcagaattt ccagcggctc ttcacgcagc agattacggt aggcttctgc
    10261 aaatgctttg cctgttccgc caataccagt atcaatcata gaccttaaga tacgggcaga
    10321 gaaagtcaga tcgggattat cgaagcaggc aaccagttca tcacacactt tctgatacgc
    10381 ttcgccgccg ttaatactat ccagcgtttg cgcgacgcgt ttcagatcgc ggaacagatc
    10441 tttacccacc tgcggtaacg ggaactgtgc ggtttcgcag ccgataccca gcgtcagacc
    10501 cggtttgcga ccttcgagga tcacccggtt ccagttaacg cgtgtacagg caagttcgct
    10561 actgctcatt tccggtgcat cagccagcgc acaccagacc ataaacaggt cgaggaatcg
    10621 cacctgctgt tcatctacac caatcggcga gaacgggttg atgtccagcg aacgcacttc
    10681 aatatattca atgccgccac gtaacagcgc atcagaaggc gactcgccgc tgcgggtaac
    10741 gcgttttgga cgaatcggcg cgtacagttc gttttcaatc tgcaacacgt tgctgttgat
    10801 ttgcagcctc ttaccgtctt tctcaatacc aatcttcgcg tactcttccg atggcgtttt
    10861 gattgcctgt ttaaggcccg ctacgtactc gtaaagatcg ttgaaggtaa taccaagatt
    10921 gctttgcgat ttattggtat agccgagatc gctcaaacga agagaggtcg catacggcag
    10981 gtaatacata ccgcactcgg ttttctcaaa cggcagcgac gttggttttc cttgcaggaa
    11041 agaagaacaa atcgccggag atgcaccaaa cagataagga atgacccaac cgaaacgata
    11101 gtaattgcgg ataacgcgga aatagcccgc agaaattttc tctttggcat cagcgcccga
    11161 gatatcaccg cacttcgctt gccagaatgc cattggcaaa gagaaattgt agtgcacgcc
    11221 ggaaatggtt tgcatcagcg cgccgtagcg atttttcagc ccttcacgat acagcgtttt
    11281 aaagcgtccg gtgttagaag tgccgtactg tgccagttcg atgtcctgac cttctgcgat
    11341 gtagcatggc atacttaacg gccacatccg ctcatcgccc atattgcgcg ccgtataacg
    11401 atgcagatcg cgcataaagg tcagcatatg ttcaatatca ccatccactg gtgtaatgaa
    11461 ttccagcaat gcttccgcaa aatcggtagt aatccatttg tgcgtcagtg cggaacctaa
    11521 tgcttcagga tgacctgttg ttgccagtgt gccatcagca ttaacacgca aagtttcgcg
    11581 ctccagccca cgctgtatcc cctttaacgc ctgaggatgt ttttccagcc aggccagcgc
    11641 ctgtgatacg tccgggatca aattgacctc ccgcctgtca aaatcgtttt aattagcata
    11701 actgtaatgg tgaccatatg tgcaggccta caattagtgc caccacatca tgccctgaac
    11761 ggtcgctgct gcaactgcaa catagcgtaa cgctttacca aggcacaaaa aaaagattac
    11821 cggtccccac gagatgcgca tccatcccgc taacagacac agtaaatcgc caaccacggg
    11881 catccagctt aataatagcg tgactgcacc atagcgtttc agccagccgg tagctttctc
    11941 ttgccagcgc gatgttttac gcaatggaaa gaaacgccca aggataacgt tagttaaccc
    12001 tccaaggcta ttacccattg ttgctgttaa aactaaaacc cagggatgac tgatcccgga
    12061 aagcaacatt gccaccagca cgacttcgga gttgccgggt aatagtgtag cgctgagaaa
    12121 actactggca aacaacgaga aaagcgataa cgcttcactc acagcaagcg aacatccacg
    12181 gcgtccatgc ctgctgcacg ggccgcctga ataccgaaat cggcatcttc aaagaccaca
    12241 cactgcgtcg gttgcacgcc catacgctgc gcgcacaaca aaaatgtgtc tggcgcgggt
    12301 ttatggtgtt tgacgtgatc agcagcgacg acggcgtcaa aataacggcg taatcccagg
    12361 tgcgccagca atgcctcagc gatggcgctt tcactccccg ttcctacagc cattgggcga
    12421 cgaccatgcc aacttttcac cacttcaaca agaggaagcg gctcgacact atccagcagc
    12481 atacttctta ctgcttctgt tttttcacgc gctaacgcat gcgggtcgag atcggcctga
    12541 ttcagctcaa taattgcctg agcaatacgc caggtgggcg atccattaag cgcaatcatt
    12601 gcctgaacat cgtactgaag accgtagtgc cctaatactt cgcgccacgc tttacggtgc
    12661 gtaggctccg tatccaggat tgtgccatcc atatcaaaaa ttaaacctgc ataacgctcg
    12721 tacatggtct tctcgcaagt cgaaaaatat gacgctactt tagcgtaatt gcttgatttt
    12781 gtcgctgatg gcgaaggaga agaaaattca ggaaatggat aaagtagtta tttagaaatg
    12841 agatattttt gaaggaaatt tttgggagaa gatggtgcat ccgggaggat tactcactgg
    12901 cgttcgccct tcgggccgtt gctgacgcaa cgttatcctc cctggtgcct gcggttaata
    12961 ccgctatctc ttcgatacct tcattgctga aggttacgga gaatatggtg catccgggag
    13021 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
    13081 gcatcgggaa aatttatttt actgctgata ttgatatcgc tactaaagcc atatcaagta
    13141 agagatggtg catccgggag gattactcgc cttgcggctc gcccttcggg ccgttgctaa
    13201 agcaacgtta tcatccctgg tgcctgcggt taattccgct acctcttcga taccttcatt
    13261 gctggagatt tcggagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt
    13321 cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaaattt attttactgc
    13381 tgatattgat accgctacta aagccatatc aagtaagaga tggtgcatcc gggaggattc
    13441 gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc
    13501 gggaaactta ctttactgca gattttttga taccgctact aaagccgtat caagtaagag
    13561 atggtgcatc cgggaggatt actcgccttg cggctcgccc ttcgggccgt tgctaaagca
    13621 acgttatcct ccctggtgcc tgcggttata ccgccacctc ttccatacct tcatcgctga
    13681 aagtacgtag aatatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
    13741 cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc
    13801 cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca
    13861 cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc
    13921 gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccta cacttttatc
    13981 gtttgcacac ttcacgttca attagtctgt aaaaacgtca aaaagagtgt tttatcaaca
    14041 gaaaaatgga ggtctgacag atagtagtaa tgcaaaaaaa tggagactta agttgaatga
    14101 acgggagtaa agcgaaaaga ctatagagtg aaggagaaat tttgagggtg cgtctcaccg
    14161 ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac
    14221 gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta
    14281 cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct
    14341 caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg
    14401 tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa tgacaaacac
    14461 attacatgta agcagtcatg gcattacatt ctgttaaacc taagtttagc cgatatacac
    14521 aacttcaacc tgactttatc gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc
    14581 acggcattgc ctgacgctta tattattgca atttcgcgct gacccagcct ttcacactgg
    14641 ctaacgctgc aggtaaggcc gcagcatccg taccaccggc ttgcgccatg tcaggacgtc
    14701 caccaccctt gccgcccacc tgctgagcga ccataccaat cagttcccct gctttcacac
    14761 gatctgtgac gtccttagat acgcctgcaa tcagagaaac cttaccttcg gctaccgttg
    14821 ccagcacgat aattgtcgac cccagctgat tttttaaatc gtcaaccatg gtacgcaaca
    14881 ttttcggctc aacaccgcta agctcgctaa ccaacagctt aacaccatta acatcaattg
    14941 ccttactgga aagatttgcg ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt
    15001 ctttttctaa ctgacgcgta cgttccagta ctgagcgcac tttatccgcc agattattgc
    15061 tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg
    15121 tgatagcgcc ttctccggtt accgcttcga tacgacgaac gcctgcagca gtacccgatt
    15181 cagagatgat gcggaacaga ccaatatcac cagtgcggct ggcgtgagta ccgccacaca
    15241 gctcggtgga gaaatcgccc atgctcagca cgcgtacacg ctcatcatac ttctcgccga
    15301 acagcgccat cgcacctttc gctttcgccg cttcgagatc catgatgttg gtttcgatcg
    15361 gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac cgcacgaatc tcttccggtt
    15421 tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt
    15481 tctgcgatac atgagtcccc agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg
    15541 agtgattcag acgaatacgg gcgcgacgag cctcatcaac atcagcctgc acagcgtcgc
    15601 ccactttcag agaacccgca gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct
    15661 gcgtatcttc caccgcgaag gagaagttag cgcctttcag ttcgccttta tcaccaacct
    15721 gaccgccgga ttccgcatag aatggtgttt gatccagcac gaccacagct tcctggcctg
    15781 cattgatggc atcaaccgct ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt
    15841 ccagatggtc atagccttta aattcagatg cactatcaac acggatcatt gcgttgtaat
    15901 cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt gctgcttcaa
    15961 aaccagcttc gtcaactttg atgttgcgct cacgacaaac atcagccgtc aggtcaaccg
    16021 ggaagccata ggtgtcgtac agacggaaag ccgtttcacc atccagcgta tcaccagaaa
    16081 gttttgccag ctcttcatcc agcaacgcca gaccgcgctc cagagtacgg gcaaactgct
    16141 cttcttcagt cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg
    16201 cagagcccat aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc
    16261 cgagcatatt gccgtgacgc actgcgcgac gaatgatacg acgcagtaca taaccacggt
    16321 tttcattgga cggcattacg ccatccgcga tcaggaacgc acaagaacga atgtggtcag
    16381 cgattacgcg cagcgattta ttgctcagat cggtcgcgcc agtgactttc gctaccgcct
    16441 ggatcaacgt gcggaacagg tcgatgtcat agttagagtt aacgtgttgc agcaccgcag
    16501 caatacgctc cagacccata ccggtatcta cagacggctt cggcagcggt tccatcgtgc
    16561 catctgcctg gcggttgaac tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc
    16621 cgtcttcttc cggacttccc ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga
    16681 tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg tcagatgcgt
    16741 atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc cctacttctt
    16801 tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac agacgctctt
    16861 tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga atggcatcgt
    16921 gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg
    16981 cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg
    17041 aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc ttgaactggt
    17101 tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg gagctgctgg
    17161 caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg
    17221 tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg
    17281 tgtcttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg
    17341 aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc
    17401 atcagatagc cacgatagag caaaaaacgc tggatcttaa ctttttctga aaagacagtt
    17461 ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg
    17521 tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc
    17581 tggcgaatac gcgcaggtcc ataacctttg cggctacggc tggcgataaa gcgcgcaaca
    17641 aatcggctgt catcgagata gccatgttca tggcaccagg caataacgcg ctcgtaatct
    17701 tctgccgtag catcaatctc ttctgggcca tttttgccca taatcggtgc cgcgagttta
    17761 cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac
    17821 aggcgagcat atgccgggcg acgggacgtt gattctgtca tggcatatcc ttacaactta
    17881 aaaaagcaaa agggccgcag atgcgaccca ggttttgctg ataaacagat ttaatagtca
    17941 atgagtgtgc agaatgttcc cgcaggccca attcgttaga ggatcgaggt gagagtaatt
    18001 tttcacgcca gtgctggcct gttcccctca ccctaaccct ctccccaaag gagcgagggg
    18061 accgtccgtg cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag
    18121 tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc gggttgctca
    18181 gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc
    18241 aggcagttgc attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc
    18301 ctgctttctc gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga
    18361 taccttcgcc gtagaggatc tgaaattcag cctgtttaaa cggtgcagcg attttgttct
    18421 tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga
    18481 tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg
    18541 tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca
    18601 gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg
    18661 ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg
    18721 ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga
    18781 tttccagtgc ctgctcgccg gtgtccggct gggagcacag caggttatca atatcgacgc
    18841 ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt
    18901 taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt
    18961 ccggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat
    19021 ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc
    19081 gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt
    19141 tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt
    19201 caatatgttc tgttgaagca attatactgt atgctcatac agtatcaagt gttttgtaga
    19261 aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac cgcatcacgg
    19321 tcaccgctga agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac
    19381 cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata
    19441 gacacggcat aatcagcacg agccgctttc agtgcaccta tcgccatttc caccacgacg
    19501 ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat catctgcgct
    19561 ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata
    19621 tcggtaatca ctttcgctac ccaaccaccg gtacaagact cggcagttgt tacggttgcg
    19681 ccacgggctt tcagcgcctg cccaacctgt tcacttaact gcatcagttc actgtcagtc
    19741 atcacaacct ctgtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga
    19801 tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaggga
    19861 agctcacgcc cccctcttgc aaatagttac tgcacgcgcg ccagcgccac ggcttgtcct
    19921 aactgccaga ccgccattgc gtaatgggtg ctgtggttat aacgggtaat ggtgtagaag
    19981 ttcggcagac cgtaccagta ctggtaaccg gtaccgacat ccagacgcag caggctggct
    20041 tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg ccgtggcaag ctgcgaaatg
    20101 ctgtacctgg ttttgaagcc atttggcaag cctggagcct gaccgtttgc cattaccgcg
    20161 acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct accgatcgca
    20221 tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg
    20281 taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg
    20341 agcggatcgt cctgctcgtc gcgcgccatc aacaggaagg tttccagctc gccagagaaa
    20401 tactccgcgc ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt
    20461 ttccccatca cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc
    20521 ggtacgccat acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc
    20581 acaccgttct gcacgttgtc cggcgtaata aattttttgc gataacggag ccatgcgccg
    20641 ttcggacctg atggcggttt caccgatgtg gttggtgcct ggttatccat cagccgcagt
    20701 accgaatcca gacgcttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg
    20761 tgtttgttca ccattttgtc gatgaactgc tgggcattcg ggttattagc gaaatcgccg
    20821 cccatctgca tcacattgtg ctgcggctca agcaggaagc cgccagacgg cgttccggtg
    20881 gtcgtttcag tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg
    20941 ggaagcaatg ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag
    21001 gggcaagtat ggtaaagcat cacgccccac acaaggaagc ggtagtcact gcccgatacg
    21061 gactttacat aactcaactc attcccctcg ctatcctttt actcaaactt tcaaattaaa
    21121 atatttatct ttcattttga gatcaaaata acacttttaa atctttcaat ctgattagat
    21181 taggttgccg tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca
    21241 ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg
    21301 ggatggtgac cggcattctt ccgctgttga ttagcctgct ggttatcatg aacgcactga
    21361 ttaattttat cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg
    21421 tttcccgtta cctactgtta ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct
    21481 taagcctggg tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcggcctctt
    21541 atagctgcca ctcaatgaat ggcctcttcc cccatatcaa ccctggcgaa ctgtttgttt
    21601 atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca ctggcggtga
    21661 gttatctgct ggttggtctg gtcaccaatt tcttccgcgg ctgggtgacc gatctgacca
    21721 ccgccatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcac ctggcaggag
    21781 caacatcatg acgcatattc ggatcgaaaa aggaacgggt ggctggggcg gcccgcttga
    21841 gctgaaagcc acgccgggaa aaaaaatcgt ctatatcacc gccggtaccc ggcctgcgat
    21901 tgttgacaaa ctggcacagc ttactggctg gcaggctatt gacggattta aagaaggtga
    21961 acccgcggag gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat tacgctgcgg
    22021 catctatccg aaacgacgta ttcccaccat taatatccac tcgacgggca agtccggtcc
    22081 gctggcgcag tacattgtgg aagatattta tgtctctggc gtaaaagaag aaaacatcac
    22141 tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc cgtgactatg acaccagtaa
    22201 gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg ggaatgggca tggggtccgc
    22261 cgttgcggtg ctgtttcaat ctggtcgtga caccatcgac actgtattaa aaaccattct
    22321 gccgtttatg gcattcgtct cggcgctcat tggcatcatt atggcttctg gccttggtga
    22381 ctggattgcc cacggtcttg ctccgctggc gagccatcca ctgggtctgg tcatgctggc
    22441 gctcatctgc tccttcccac tgctttcacc tttcctcggc ccaggcgcag ttatcgcaca
    22501 ggttatcggc gtattgattg gcgtgcagat tggtctcggc aatattccgc cgcatctggc
    22561 tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc cggtcggttt
    22621 gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg tactggtgag
    22681 ccgcttttta accggcgcgc cgactgtact gatcgcctgg tttgtctccg gttttatcta
    22741 tcaatagagg ctgaaacatg accgttattt atcagaccac catcacccgt atcggcgcga
    22801 gtgccactga cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc gcgccagcgg
    22861 acctcgaaga gtattgcttc attcattgcc acggcgagtt gaaaggtgca ctccatcccg
    22921 gtttgcaatt ttcactcggg cagcatcgct atccggtgac cgctgttggc agcgtggcgg
    22981 aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta aacgaagcgg
    23041 aatttccggg cactgtccat gtggcaggcc ctgtccccga cgatatcgcg ccgggatcgg
    23101 ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt gtcatcggtg
    23161 gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg tatcgcgtcg
    23221 cggttgtcga tattcagagc gacaaagccg caaatgtggc acaagaaatt aacgccgaat
    23281 atggtgaaag tatggcgtac ggttttggtg ctgacgccac tagcgagcaa agcgttctgg
    23341 cgctctctcg tggggtagat gaaatctttg gtcgcgtgga tttgctggtc tacagcgccg
    23401 gaatagcaaa agcagccttt atcagcgact tccagctcgg cgattttgac cgttcgctac
    23461 aggtgaatct ggtgggttat ttcctgtgtg cgcgtgaatt ttcgcgtttg atgatccgcg
    23521 acgggattca ggggcgcatt attcagatca actcgaaatc cggcaaagtg ggcagcaaac
    23581 acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg gctgactcaa tcactggcgc
    23641 tggatctggc ggagtacggc attacggtgc attcactgat gctcggtaac ctgctgaaat
    23701 cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa gctgggtatc aaaccggatc
    23761 aagtcgagca gtattacatc gacaaagtac cgctcaaacg cggctgcgat tatcaagatg
    23821 tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc gtactgcacc ggacagtcga
    23881 tcaatgtcac cggcggtcag gtgatgttct gatcaacagc ggagatccat taaggatctc
    23941 cgtgagacta tagaatgcct gatgcgctac gctcatcagg catacaggac ttccgccact
    24001 acattaagga aaagttatgg tatccgcact catcaccgtc gccgttatcg cctggtgtgc
    24061 gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg acacactatg
    24121 ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc tttaaaccgc gggtcgtggt
    24181 cgccatcgcg ctggacgatc agcagcgcat cgtcgacacc ttgtttatga aaggactgac
    24241 cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt atgcatgcgg gtgatttaca
    24301 gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat tggcgcttaa
    24361 actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat cattttgaaa
    24421 cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca ttctggagta
    24481 tctgcaaaag cagggtaaat gctcggttga agaattggcg caatactttg acaccacagg
    24541 cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca ttcgtactta
    24601 tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata aaacactcat
    24661 caacacccac aagaaagagc tgattgcaga agctgccgtt agttttatcc atgatggcga
    24721 ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg gttcccctgc tctcgcgctt
    24781 taataacatc acggtgatga ccaacagcct gcatatcgtc aatgcgctat ccgaactgga
    24841 taacgaacaa actatcctga tgccaggcgg aacgtttcgt aaaaaatcgg cctcatttca
    24901 cggacagctg gcagagaatg ccttcgaaca tttcagtttc gataaattgt ttatgggcac
    24961 cgacggcatc gatctcaatg cgggcgtaac caccttcaac gaggtttata ccgtcagtaa
    25021 ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa agtttggccg
    25081 taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctgatta ccgacgcagg
    25141 tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga tcataaccgg
    25201 agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg ctggagttgc
    25261 aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca
    25321 tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc cacattggta
    25381 agaaaatcgc cgcaacactt gccagtaccg gcacaccggc tttttttgtc catccggcag
    25441 aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg tttatctctt
    25501 actccggtgg cgcgaaggaa ctggatctga ttattccgcg tctggaagat aaatctatcg
    25561 cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggca aaagcggtgc
    25621 tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg
    25681 tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg cgcggattta
    25741 atgaagaaga ttttgcccgc tcccacccag ccggggcact gggcgctcgc ttgctgaata
    25801 aagtgcatca tctgatgcgc cgtgacgatg ccatcccaca ggtggcgtta accgccagcg
    25861 tgatggatgc gatgctggaa ctcagccgca ccggtctggg gctggtggcg gtatgtgacg
    25921 ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg ctggttggcg
    25981 gcggcgcact caccacgcca gttaatgaag cgatgacgac aggcggcacc acattacagg
    26041 cacaaagtcg cgccatcgac gccaaagaga tcctgatgaa gcgcaaaatc actgccgcac
    26101 cggtggtgga tgaaaacggc aaactcaccg gcgcaattaa cctgcaggat ttctatcagg
    26161 ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg ttggcgacgt
    26221 cggtttccag catccgcgcg caggcagccc agttgtgatg attttgtgcc agtgcctggc
    26281 gaatggtttc acgctggaac gcttctgtcg cttcacgcag gttttgctta acaacgggca
    26341 ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt tgtgcctcaa
    26401 gaatcacttc atcgccgctg cgagtggctc tcgccagcac taccgcccga tgaatagcat
    26461 gttccagttc gcgcacgttc cccggaaaac gatagtgttg cagtaaattt cgcgctccgg
    26521 cacttaatac cacgcgggag agccccagcc gcaaacgaca ctgctcgcag aaataccccg
    26581 ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt ggaaacacgc
    26641 tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag cacctcttcg cgtaagtcgc
    26701 ggttagtcgc cgccagcacg cgcacatcga cccgcaaact acggtcatcg ccaacgcgct
    26761 gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac gacaactcgc
    26821 cgatctcatc gagaaacaga gtgccgttat ccgccatttc aaacttcccg ctgcggttac
    26881 tgatagcgcc agtaaacgct cctttcacat gcccgaacaa ctcactttcc gccacacttt
    26941 ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc gaggcttcat
    27001 gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatc aggacgttga
    27061 gatcggacgc cgccacaatc tcaatctctt ttttcagttg cgtcatgcca ggggacaagc
    27121 caatcatctg cgtctgtttc accgcttcaa acggcgtggc atcgcctggc atcatattct
    27181 ggctttccag ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc agcgcagcaa
    27241 tcagccgtag ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg
    27301 tcagtgcgcc gatcaggttt tgcccggcaa acaatggcag accaacgcag gcgtgaacct
    27361 tcagactctc ctgcccagga atcaaaccgt catagggatc gggcaattcg ctgtctgcag
    27421 gaaagcgcac cacgtccccg gcgcgggcaa tcgcttcaag ccgtggatgc ccttccagcg
    27481 caaagcgtct accgagtaca tcctttgcca gaccgtcgat ggcaagcgga ataaactgcc
    27541 gcgaatcgta acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga
    27601 tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg gcgatattcg
    27661 ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt
    27721 catatcgacc atttgattga tagtcatttt gactacccat taatgagcat acttttattt
    27781 atatagtaaa aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt
    27841 tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa ataacattca
    27901 ttgggttggt caacgtgact gggaagtgcg tgattttcac ggcacggaat ataaaacgct
    27961 gcgcggcagc agttacaaca gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac
    28021 cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc
    28081 ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgctgggg cgctgaccga
    28141 actgatggcg caaattcccg atacgccgat ctactgtact gccaacgcta tcgactcgat
    28201 aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg gcgacacgct
    28261 ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc actggccgga
    28321 cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg ctttcggtca
    28381 gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc ttttcgagca
    28441 gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa cgccgaaaat
    28501 taccgagatc ctgggcttta acttaccagt cgatatgata gccacttccc acggcgtggt
    28561 atggcgcgat aacccgacgc aaattgtcga gctgtacctg aaatgggcgg ctgattatca
    28621 ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca tgatggctga
    28681 cgctatcgcc caggggattg cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt
    28741 cgcccgaagc gataaaaacg aaatcctgac taatgtcttc cgctcaaaag gcgtgctggt
    28801 cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg tggaagagat
    28861 gactggttta cgcttccgta acaaacgcgc cagtgctttc ggctctcacg gctggagcgg
    28921 cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgcttag
    28981 cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgtcgtg aacacggtcg
    29041 cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga atacggtggt
    29101 taaagaagaa acctctgcca ccacgacggc tgacctcggc ccacggatgc agtgcagcgt
    29161 ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg cgccaggaac
    29221 gccgtggagt gaagtcccgg ataacttcct ctgcccggaa tgctccctcg gcaaagacgt
    29281 ctttgacgaa ctggcatcgg aggcaaaatg agtaacggca ttgtgatcat cggttcgggc
    29341 ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccactat tccattaacc
    29401 ctgattgccg ccgacagcat ggatgagtac aacaaacctg acctcagcca tgttatcagt
    29461 caggggcaac gtgccgatga ccttacccgc cagacggcgg gtgaatttgc cgagcagttt
    29521 aatctgcacc tgtttccaca aacctgggtg acggatatcg atgccgaagc ccgtgtggtg
    29581 aaaagccaga ataatcagtg gcaatacgac aagctagtac tggcaaccgg tgccagtgcc
    29641 tttgtcccgc ctgtgcctgg gcgtgagtta atgctgacgt taaatagtca gcaagagtat
    29701 cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgttgg cggtggtttg
    29761 attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac gctaatcgac
    29821 aacgctgcca gtattctggc gtcattaatg ccaccggaag taagcagccg cttgcagcat
    29881 cggttgacgg agatgggcgt tcatctgttg ttgaaatctc agttgcaggg gctggaaaaa
    29941 acagattctg gcattctggc aacgctggac cgccagcgca gtatcgaagt ggatgcggta
    30001 attgccgcca ccggactgcg cccggaaacc gccctggcac gacgcgccgg gctgacgatt
    30061 aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat ttacgcgctg
    30121 ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tccagccgat tcaacttagc
    30181 gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact accggcgatg
    30241 ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag
    30301 gatttacgct ggcaaattaa taccgaacgc cagggaatgg tggctcgcgg tgttgacgat
    30361 gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc atttggattg
    30421 ttgaaaacat tgccgatgta ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc
    30481 gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga taaggcgttt acgccgcatc
    30541 cggcaatggt gtccaaatgc aacacgtttt atccgttctg gacttcaccc gctaaccaac
    30601 gcgccgcagc aataaccccc tgccccagag acaaaccgcc atcacccgcc ggtaaactct
    30661 gtggaaagag caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc agcaaacggt
    30721 tatgaataac cccgccgcta aataccagcg tagtgatacc acgcatcgtg gcctgctcac
    30781 gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa
    30841 ccggtgcctg ccagttcagc cactgctgcc agaaagtggc gagatccagt tgattgtcca
    30901 ccagcggcat tgtcaccgga tgcgtcactc cgtggcacga agctgcgagt gcctccagag
    30961 cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt gccgccgcca
    31021 cagcatcgaa caaccgccca cacgatgacg ccagcggcgc gttaattcca cgctcaatgg
    31081 cccgcgccag cacgctccag ttttgctgtt gcacacttgc tgtttcagaa taattctgcc
    31141 actccggcac aaagcgcagg cactgcgcca gcaggtttcg ccacggctgc ttcgccgcca
    31201 aatcgccacc cggaagcgcc actgcaggca agccgcccag gtgctcacat tcgcgatagt
    31261 tcacccgcag acactcgccg ccccacaaag cgccgttctc ccccataccg ataccatcga
    31321 gcgtcaaagc aatgacatca ccgccatcca gcggccactg atgctctgcc agacacgccg
    31381 ctgcgtgggc atgatgatgc agcaccgttt gcgtcggcat attcatttcg ctcgcccact
    31441 ggctggagac atagcccgga tgcacgtcat gcacaacgta ttgcggagtg aaatcgtaga
    31501 tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg tcacttaaat
    31561 cgcccagatg ctgactcaac accgcttgtt caccgcgcac caggcagaag gtgtttttca
    31621 gatccgcgcc aagacacagc acaggcggaa catttttaaa gcccggaggc aaaaccagcg
    31681 catccggcac atacccgcgt gaacggcgca gcatttcgcc gctttcgcgc accaccgaat
    31741 catccatccg ctgcacgatg tcgcggttat gtatcaagaa tccgtcggca atgccctgca
    31801 aatcctccag cgcctgttcg ttgctgatag ccggtggttt accgctcagg ttgccggagg
    31861 tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg ttcgcaggca
    31921 acattgcccc gacttcgtta aggtcagggg cgatatcatc acaaagctca ggaacgtatt
    31981 ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag caactggcgc gcagcgtctg
    32041 gtaaaccgtc agccactggc aacatgaccg ccagcggttt cgccgggcga tgtttgcgcg
    32101 cccgaagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga aatccgccaa
    32161 tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagccgcc tgtaatgccg
    32221 cctcttgttc tgcatgttca ccatgactta cccattcaag atgtgggcca cactccgggc
    32281 aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc gcggtactct ttgtcacaag
    32341 ccggacacaa cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc atggcgcgaa
    32401 taatggtgaa acgcgggccg cagtgggtac agttgataaa cggataacga taacgccgtt
    32461 cgcctggggt attcatttcg gcaagacagg cagggcaagt agcggcatcg ggaacaattt
    32521 gcgtattcat ggtgccacct gtgctctggc gtatagtgaa ctcggtgggc agttgtgacc
    32581 agatgtacgg ctcacgctcg acgctatcaa tacgcgccag cggcgggcag tgctgatgca
    32641 attgaacaag aaacgtttcc gggtcttcca gcagccggac ttctacgcca tcgccgtcat
    32701 tacagacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac
    32761 cgacgccctg cactttgcct cgaatacgca gttggacacc gcaagatgtg ttttttgcca
    32821 ttgagttatt cccgccatca tgaattgcgt aacccgccct gccggacacg actgcgtcgt
    32881 atccggcagt ctcaggtcgg cgataccgcc agctccgtat tctacgaata tttccgggaa
    32941 ttcctttgat gccagaacag ttctataaga tttttagaac atcagcgccg tacggcgacg
    33001 tttttctgcg ctcagttgtt caagtttatt acgatcgaca caaatcagcg catgggtcgg
    33061 gcaagccgcc atacacgccg ggccgtcttc gcgatggttg cacaggtcgc atttattggc
    33121 ttcagctttg tcagcccgta cattcagccc cgcgccgctg ttgcggatca ccggacgtac
    33181 caccacttcc atcgcgccat acgggcaagc caccacgcag gttttgcaac caatgcaacg
    33241 ttcctgcatc acatgaacaa accctttatc acggctgata gcaccattcg ggcagacgtt
    33301 agcgcacggt gcatcttcac actgacggca gactgtcgcc gtggaaatgt tcacaccttt
    33361 aatgacatgg attcgcggta aaaaggtttc cggggtcagc gatgcacagt cctgattttc
    33421 ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc
    33481 agcaatgatg aaacggttca tcaaattctc cagcaatgac agttaatgcg ccgatacatt
    33541 cacaaatcat gccagttttt aatttgctgt tatttaagga aattaatttc tgtaatgcag
    33601 gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa
    33661 gggcaatgag tgagtcgcgg cggatcagtt ttccgctgaa ggttttcggc ggtgagaaat
    33721 ccccgccatc gagcataaaa atcagccgtc caataatttc ctgaatcatc tctgttaccg
    33781 gaatttttac gctggagagc gccggaacgg tgtagggggc aattgcgata tcatcgaatc
    33841 cgataaccga cacctgctct ggcaccgcta cgccgcgctc gtgcaacgct ttgatcgcac
    33901 ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccca cgttcgagca
    33961 acgtttctac cccttcggcc ccgctggcgg gcgtccattt accgttagcg ataagttttt
    34021 cattgagcgc aataccatgc tgcgacagcg cgtctttata cccggcaaga cgttcaacgc
    34081 tggtggggga atccatcgag ccggtaagga aagcaatctc ctgatgtccg gcgtttatca
    34141 actctgccac ggcattaaag ctggtctgtt tatgatcgca ccagacgcta tggctgctgt
    34201 ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgcgcgtca atgatgtcat
    34261 caatctcatc cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca
    34321 gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct gccaatagca
    34381 actgccgccc tttctcttcc gccattcgcg cggcatgaaa cagtaattca ctaaaataaa
    34441 tgccgtgata aagcgtgttg gtcactacca gccccagcgt ctgagtactc ttcgccgaca
    34501 gattgcgcgc cagcaaattt gggcggtaac cgctctcttc taccgcctga aacacgcgat
    34561 ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg gtcgcttttg
    34621 aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc cctttacacg
    34681 caatcaacgc agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg cgggaaaaat
    34741 attcaggtga ccggtttcac aaatataaaa aatgaacaat taactctctt gcttatttag
    34801 tgacaactat tcatgatttt gtgaaaccgg tttcctaatt ccgtttcagc atcggcattt
    34861 ttccgttacg tcgactgata acaactacat ctaccctact gataacagga taaaatccga
    34921 tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc ggcgttgata
    34981 acatctcggc agtcacgcac tgtatgacgc gtttgcgctt tgttatcaaa gatgaccagc
    35041 ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta cgtagtgaca
    35101 accagtgtca ggtgattatc ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc
    35161 tgctgccggg agatatgcag cccgcacagc ccgtgggtaa acccaaactc acgctgcgtc
    35221 gcattggtgc ggggatcctc gatgcgctga tcagcaccat gtcaccgctg atcccggcga
    35281 ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgttga
    35341 caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct ttcttcttcc
    35401 tgccgctaat ggtcgcggca tctgccgcca tcaaatttaa aaccaatatg tcgctggcga
    35461 ttgccatcgc aggtgtgctg gtacatccga gctttattga actgatggcg aaagcggccc
    35521 agggtgaaca tgtcgaattt gccctgattc cggtcaccgc ggtgaaatat acctacacgg
    35581 tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca
    35641 ttacaccggc tgtgaccaaa aacttcctca agccaatgct gattgtgttg attgccgcac
    35701 cgctggcaat cctgctgatt ggcccgattg gtatctggat cggtagcgcc atttcggcgc
    35761 tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg ggcgcgctgt
    35821 ggcctctgct ggtaatgacc gggatgcacc gcgtctttac gccaaccatc attcagacca
    35881 ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct aacctgtcgc
    35941 tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa cccggaactg cgccagacgg
    36001 cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg ttatacggcg
    36061 tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt atttgcggcg
    36121 cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccgggg ttatttacca
    36181 gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg
    36241 cgctggcggt ggtgctgtcg tttatcctca cactattgct cggctttgag gatattcctg
    36301 ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgc acaaccgacc gtcgccaaag
    36361 aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaggttttt tatggggcgg
    36421 cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa ggtggcaaag gactgaccac
    36481 ggtcgatatg atcccgcacg gcgagcatcg aatggcggtg aaactggggc tggaaaaacg
    36541 ttttcagttg cgcgatgacg agttttatcc cagccatgag gcgacggatt tttatcatcg
    36601 ttataaagaa gatatcgccc tgatggcaga gatgggattc aaggttttcc gtacctcaat
    36661 tgcctggagc cgcctctttc cgcagggcga tgaaatcacg cccaatcagc agggcattgc
    36721 tttttaccgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc tggtcacgtt
    36781 gtgccacttc gatgtgccga tgcatctggt cactgaatat ggctcctggc gtaaccgcaa
    36841 gctggtggag tttttcagcc gctacgccag aacctgcttt gaagcatttg atggtctggt
    36901 gaaatactgg ctaaccttca atgaaatcaa cattatgttg catagcccgt tctccggcgc
    36961 gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg ccgcgcatca
    37021 ccagctggtt gccagtgcgc tagccaccaa aatcgcccat gaggttaacc cgcaaaatca
    37081 ggtgggctgt atgctggcgg gcggtaactt ctacccttac agctgcaaac cggaagatgt
    37141 ctgggcggcg ctggagaaag atcgggaaaa cctgtttttt atcgatgtgc aggcgcgcgg
    37201 cgcgtatccg gcttactctg cccgcgtatt tcgcgaaaaa ggggtaacca tcaacaaagc
    37261 accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt gtctctttca gctattacgc
    37321 ctcgcgctgc gcctcggcgg agatgaacgc caacaacagc agtgcggcga acgtggtgaa
    37381 atcgctgcgt aatccgtatc tacaggtgag cgactggggc tggggaattg atccactcgg
    37441 tctgcgtatc accatgaata tgatgtacga tcgttatcag aagccgctgt ttctggtgga
    37501 aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc gagattaacg atgactatcg
    37561 catcagctat ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag atggcattcc
    37621 gctgatgggc tacaccacct ggggctgtat tgatttagtt tccgcctcta cgggtgaaat
    37681 gagcaaacgc tacggttttg tctacgtaga ccgtgacgac gcaggcaacg gcacgctgac
    37741 gcgcacgcgt aagaaatcgt tctggtggta taaaaaagta attgccagta atggggaaga
    37801 tttagagtaa gtaaccgtgc cggatgcggc gtgaacgcct tatccggcct acatagttcg
    37861 tgcgacatgt aggcccgata agcgcagcgc atcgggcaat ctggggtttg gcatcagtca
    37921 aaatcaaaga aaccattccg gcttaacgcc aaatcgggct gaaagtgcct taatgtggga
    37981 tattgtcagg gatcgttgtc cagacaaaat ctgactgacc agggatttag agccgatttc
    38041 gttcttcaaa tccgcatagg tcagattatg ttgatcaatc aacgtgcgaa gtaacgccac
    38101 gccaaccggc attgccgcaa cggctttgtc gaactctgca aacttttcgt tgttgttttc
    38161 atactctgca atgcggctcg ccagaaagtc gataagtgga ttggtgtcat cttcttcaat
    38221 caaatattcg accagttcca gtgcttcacg ataatcatct tctgagtcac tgcctcccag
    38281 aaaggggact gcattgacca gttctcttgt tgctttaaca gcgcgtgcag cattagcagt
    38341 cattctttgt tctctctgta gtaacgtgac gtctgtctgc acgtaatccc cctcatagcc
    38401 actctgcaaa aagtcagttc tggttcaccc cataaagcac atcccccttt atcaagcaac
    38461 gctgcgtctg cgccagatct tccgtttgcg ggtcgcgccc ggcgatgggt ttgaggacga
    38521 gtttacggtt gggggtataa gtaaaacgcg tgagcgcggg atagcttaac ttgccaaacc
    38581 agccgtgaac acgctgccat ttggcgtctt ttacctgata cagactggtt atccagtaac
    38641 cggaatcaaa ctgcatatcc agcaattgcg tgtggccatt gccttgcaaa tccaacaaat
    38701 catctacgcc agttttgctt gcggtgtaga atcccctggg ttcaaagaca cagggacggc
    38761 cttcgcgcgt gaacgtcata agaattaaat gtgcataagg agccagacca ttaccggtac
    38821 ttggtaacca gataaccaga tcctgaatgc cgttacgatc aagatcggcg cgatagagtt
    38881 gccctttgcc cgctatgttt gccagcatac ccacaggcac agtccaggat ttaccggcgc
    38941 ggtctttagc ggttaattgc cagtaatctt cttcatttgg catcttttca agcgtcacgc
    39001 cagcaacgcc gacttttcta aaatgccagt tttgctcggc atcataaggc atggtcagga
    39061 gaggtttcag cgcccgcttt tgcgcgtatt cgctgtcact catcggcaga taatcggtaa
    39121 agctggtggc ctgtgcaggc agcactaagc tcaataaaaa agcgcacatc cctgttctga
    39181 tgttcaattc cctgaattcc ttaatgaaaa actactctac ttccaccgct aactgcgcaa
    39241 atccaccatt tccttcccag ccttccagtc gttgataaac ggtttcaacc gcatctttaa
    39301 tcggctgggt catcgggtag taaaagccga cgatatccgg ctggatgccg aggaaaatca
    39361 cttcgccaat atcttctttc aactggtcga taaggtagtt caacggcatg ttatgggtag
    39421 tcatcataaa catctcggcg atatcatccg ggtcgatgat gcggatttcg ccggggttta
    39481 gccccatatc cgtggcgtcg acaatcagca gtcgtgtcgg gcgcagttca cggatggcga
    39541 cgatgtcgtt ttccggtgca ctaccgccgt caatcaccac ccagttacct ttcggcgcgg
    39601 cggcgcactt ttccgccagc agcgggccag cgccatcatc gcccatcatg ctattgccaa
    39661 cacagagtaa aacgtcagtc acgcagtctc ctcaccatca aatagatggc gttttcctga
    39721 tgaatatcat gcagcatact tagcagcatt gtactccatt cctgctgctg cgaagtttgc
    39781 gctgcccggg ctttatcgaa tgcgttggcc agcatcggca catggttaat gtcgatgacg
    39841 atctcaccat atttcggcac gccttccatt ttgcggcggg cttcgctgcc cgcctccaga
    39901 gtggcaatcc atgccagata ttcatcccac gggcaggtga gcgccgcttc caggcaatcg
    39961 ataaccccaa ggtggtgacc aatcgccagg ctgtaataga ccacctgctg cgcctcggcg
    40021 ggcgtggcat cgttctcatc aataaattta cggctcagtt gactgaacac caccttttca
    40081 ctcatcggat acgcgcctct tctacaacat gattcagatg gctgacaatc tcgttcagac
    40141 gcggatcgtt ttccgcttcc agccagcgtg caacctgttc ttcgccctgc cctaactgtg
    40201 tcaggaagtc atcggcaatc tgacgaccgt aacgataacc tgccaggcgg cgtgcttcgc
    40261 gatccacttt cacacgcagc ggctgcacca tatcgccatg caggatttcc gccggttgtt
    40321 catcctgctc acccgggcca cgggcgtgaa ttttctgctc cagcaggccg agcgccattg
    40381 caaagccgta cagcgtggcg gcaggcgttg gcgggcaacc agggatgtag acatctaccg
    40441 ggacgatttt atccgtaccg ccccacacgc agtagagatc gtggaagatc ccgccactgt
    40501 taccgcaggc accgtaggag atacagattt tcgggtccgg cgcggactgc cacgcacgca
    40561 gcgcagggga tcgcattgca cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac
    40621 gcggtgaagg aacgacttta atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg
    40681 cgaaaatttc gatttcgcag ccgttgcagc cgccgcagtc cacgcggtaa acgtaggcag
    40741 aacgtttgat ttttttcagt aacgacgcct tcatgctggc gatggattca tccaccgtca
    40801 tcgggaccgg aatgccgttg gcgtcacggg ggcctaataa attgctcatc agatggcctc
    40861 tttcatatgg cgagtcagtt caatacggtc ggacggcacc aggcatttct ggcgcttaca
    40921 ttccgggcaa gtctcaaagc tttcgcggtg gttttccgcg cggctgtcac cattgtgctt
    40981 aagcagcgca atggcgtagt cgatctcttt ctggacggcg aaaggacgat tgcagacgcg
    41041 acagctgcac agcacaaaac gggactgttg caggaaatct tctttcttcc acaccgccag
    41101 ttcgtactct tgcgacagtt tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca
    41161 gaagatgcag cgcccgagat taaactccca cgcaagctct cctgtggcga ggtcggtttc
    41221 aaccgttaaa gcgtttgacg ggcaggcatt gacgcaggcc gcgcagccga tgcactgctg
    41281 cgggttctgc tctggcttac cacggaagtt tttatcaacc gcaatcggct ccagcggata
    41341 agacgaggtc gccgtgccgg ttttgatgac ttttttgata aaggtaaaca tggcgattcc
    41401 ttatttcagc ggcgagtttt tacgctcaat gctgtaacgc tcgagttctt tgtacggcac
    41461 cactttgctc ttcttcttac gtacatcgac caccgtcatg cggtcggtac aggagtagca
    41521 agggtccagg ctaccgataa tcagcggcgc atcagaaacg gtgttgccac gcagcatgta
    41581 gcgcagggtc ggccagttcg cgtaggtcgc ggcacggcag cgccagcggt acagcttctg
    41641 gttgtcgccg gtcatgctcc agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag
    41701 cgcgaaacgg tgcggaatgt aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt
    41761 atccagaccg tagtcgatca tgttcagcgc ggtatagacc tcgttgattc gcactttcag
    41821 acgggaaata acgtcgcagc cctgctcgct gtggacttcc attggcagca ggccataacc
    41881 aacaaacggg tgatcggcgc gggtatcgcg agcgtgaccg ctggcacgga ccatcgggcc
    41941 aacgttactg aagtcgcggg caatttccgg gtccagacga ccaatgccga cggtgcgctg
    42001 ttccatgttc ggcgtactca gcagcacatc caccagctcc tgcacttcac gacgcatctg
    42061 ttgcgccagc tggcgggtct ggatcatgtc atctttcaac agatcgcgac gaatcccgcc
    42121 gatcaggttc aggccgtagg ttttacgcgc accggtgagg atctctgcca ttttcatgga
    42181 ggtttcacgc acgcggaaga actgcataaa gccggagtca aagccggtaa agtggcaggc
    42241 gagaccgagg ttgagcagat gcgagtgcag gcgttccacc tccagcagaa tggcgcggat
    42301 catctgcgca cgttctggca cctgaatacc catcgcgttt tctaccgacg tggtgtaggc
    42361 ggtgctgtgg gcaaagccgc agatcccgca cacacggtca gagaggaagg tcacttcgtt
    42421 gtaacccata cgggtttccg ccagtttttc catgccgcga tgaacgtaga acagacggta
    42481 gtcggcgtcg ataatgtttt cgccatcgac gaacagacgg aagtggcccg gttcgtcaga
    42541 agtgacgtgc agcggaccaa tcggcacgac gttgtttttc ttgtcgccca gttcgttgat
    42601 gaactcgtag gtttcagcat cggtagtcgg tgccggacgc tgacgataat ccatgctgtc
    42661 tttacgcagc ggataaagtt catccggcca gtcatccggc agcaccagac gacgttcatc
    42721 cggcagacca accggaatca aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc
    42781 cgccggaacg cgcggcgtca cggacggata ctccggtttg ttggcgtcaa cttcgacgcg
    42841 aaccgttacc caacacttgg tgcccttctc catcgacagc acgtaataaa cggcgtaatg
    42901 accattcagt ttgcgttcgt cgttaccaaa cagcaccgac agccagccac cctgcttgta
    42961 gtagagaaac tccaccactt ccggcaggta gttcaccttc acggtcacgg tcagctgatc
    43021 tttggtctgc caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag
    43081 ataatgttga cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc
    43141 aaggagacga acgctaaaaa tgcaaagcca aacccagccc aggtaatgcg cggagtaata
    43201 tcaagacgca gacgggccat gctgttttcg aacagcgcga taaccaggac gccgacgacc
    43261 agtttgacga tggcaatcac cagcgccagc agcagcccac cggcggtgaa ggtttccatc
    43321 tgtccccacg ggataaatac gccgacgaac atctgcagca ccaccagctg tttcaggctg
    43381 ataccccatt tcatgacgcc aaagccgctg ccgctgtatt cagagagcgg gccttcctgc
    43441 agctcctgct cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaggtggcg
    43501 aacgcacagg cacaaagcgc cagtaccagc gggatgctct ggctcagcgg ccagtgataa
    43561 acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc gacccacaga
    43621 ccaagcagca gcatcggttc gaccagcacg ccaagcatcg cttcacggct cgcgccgata
    43681 gcggtaaacg ggctaccggt atccagaccg gaaatggcaa agaagaaacg cgcgatggca
    43741 aagagataca gtaaggtgat taaatcaccc agttgcggca gcggagaacc gacggtcacc
    43801 accggcagcg cagtagcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg
    43861 aacacccagc cggaggcatc cgggccaacg ctctggcggc ccagcagttt gaaaatgtca
    43921 cgatactcct gcaacacacc cggcccacga cggttatgca gacgggcgcg cgccacgcgg
    43981 gtaataccgg agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat
    44041 aaaacactca ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa
    44101 ccagcgccat ccggcggaac agcaacgcac taccttcgca ctgccagccc ggcaccagcg
    44161 acaccggatt cagccatttg cgcagtttca gcactggcgc aaacgcctgt ttaaccggca
    44221 tggcaaaacc gtgagcggta atcaccattg atttttcatg gtcgtagccg cacacccagg
    44281 ccgcaccgcg ggaacgtgac ggcaggcggt cgcctttgca aatcgccata atgatgaatg
    44341 gcagcagcgg gcaggcaatc agcagcaatg tgatcatggg ttgagaaacg gtggtgttag
    44401 caggctccag aggcagaggt acagcagcag agagcatcgg cagcagccac ggcgcagcaa
    44461 caccgccaat tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg
    44521 cacaggtggc gttttcggct tctttggtac gcggcgcgcc gaggaacgtg acgccataga
    44581 ctttcgccat acacatcacc gccagcgcac cggtaattgc cagacccaca gcgagcagcg
    44641 gccccagcag acgggcaaca aacgcgccac tattgctcag tttgaagaag gattgataga
    44701 taacccattc cccggcaaac ccgttcagcg gcggcagcgc agccattgcc attaacccga
    44761 ctaacatggc gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat
    44821 cgcgatgacc ggtacggaac cagacgctcc ccgccccgag gaacagtacg cttttgaaca
    44881 ggctatggtt aagcagatgg tacagaccac cgaccagacc aagagcaatc agcgccggtt
    44941 gttcgagcgc gatacccgtt acgccagcgc ccagccccag caggataatg ccgatatttt
    45001 ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca tacagaccac
    45061 cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac agcggcgcgt
    45121 taccacccag cagcgacagg gttaaaatgc ccagcaggcc aattttcatg actaccgtag
    45181 aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca
    45241 gcggaataat cccggccagc aggccaaagc caatcacacc gagcagccag atatcggaac
    45301 cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg ccataacgct
    45361 gccacagcag ccagcaagca atcgccagca gcagagtgcc aagacgcccc agcgcaaacc
    45421 acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc atgacttcgg
    45481 ccattaccac gaacatgccg aggttgctgg caatgacggc gcagacggcg gcagccatca
    45541 acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttat
    45601 agaggctgac aaacagaccg caaaggccga gggtaatcag ccaaatcgcg ttaagcgggg
    45661 agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca
    45721 gcacagtgaa gcccgcggct gccgtataca ggctaccaac cgcgccgcca attccggcta
    45781 tccagccact tagcgtcttt tgaaaagaaa agagaaatgc cagaacagcg gcggcaataa
    45841 accacgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc
    45901 ctgctgaaac aaggtgagat cgccaaagtc cgtattaaaa gtcagctcac gcttacgttt
    45961 gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg
    46021 cacgcaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg cgatcgcgcg
    46081 aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg
    46141 cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat
    46201 tgcgccaaac gggcaggcga tgccgcacag cttgcagctt acgcacaggc tttcattcaa
    46261 ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg cagaccaccg cgcagggtgc
    46321 atcttcacag tggtgacaga gctgcggcgc agattctttt tcattcagca tcactctcag
    46381 gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt
    46441 gtggcagccg atacagagcg tggagtcagc aattacaaaa cgattcacca ggcattcctc
    46501 aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga
    46561 cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg
    46621 ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt tcaatagcgg
    46681 tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc
    46741 gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa
    46801 tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa
    46861 agccatccgc cagggtgacg taaatgcgaa aatgttcaaa aaggacgaaa cagagttctt
    46921 tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa
    46981 ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt
    47041 aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat
    47101 gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag agatttttaa
    47161 gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca
    47221 ccgcagggat aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag
    47281 gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg
    47341 caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg
    47401 tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag
    47461 gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt
    47521 atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac
    47581 agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga
    47641 tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc
    47701 ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca
    47761 ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg
    47821 gtcatggcga agcgggcact cacgcaccgg gcatgagcca gcgtcggatg ctggaagtcg
    47881 aaattgacgt gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg
    47941 cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc
    48001 tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc
    48061 agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga
    48121 acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc
    48181 cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca
    48241 gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca
    48301 aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg
    48361 acctgttgcc gtatctcaac tttgacgttg aaaagtgcat cgcctgcgcc cgcgaagtca
    48421 atccagaaat tgaaatcatc cttatttccg ccaccagcgg tgaagggatg gaccagtggc
    48481 tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga
    48541 cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt
    48601 cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg tacacgttgg
    48661 ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa
    48721 catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt
    48781 tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa
    48841 cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt
    48901 ggtcataccc acgctatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag
    48961 tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat cgacacctgc
    49021 gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgta
    49081 ccagggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt
    49141 tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgcaa agtggtgttc
    49201 ttcggcttag gttttgaaac caccatgccg accaccgcca tcactctgca acaggcgaaa
    49261 gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat cccgacgctg
    49321 cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt
    49381 agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg
    49441 gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct ggtggagcag
    49501 aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt
    49561 aacctgctgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga cagcgaatgg
    49621 cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc
    49681 gatgccgaag cacatttccg cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt
    49741 tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc
    49801 tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg
    49861 tggtatcagt atcgtcagca ggagaatgaa gcgtgaataa tatccaactc gcccacggca
    49921 gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc
    49981 cgtggctggc agagcaggaa gaccaggcac gtcttgatct ggcgcagctg gtagcagaag
    50041 gcgatcgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg
    50101 gtaatatcgg caagctggcg atttgcggca ccgcgaatga cgttgcggtc agtggcgcta
    50161 ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga
    50221 aagccgtagt aaccagcatg gcagaaaccg cccgcacggc aggcattgcc atcgttactg
    50281 gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca
    50341 tgggcgcaat tccggcgaat attcattggg gcgcacagac gctaaccgca ggcgatatgt
    50401 tgctggtgag cggcacgctc ggcgaccacg gggcgactat ccttaacctg cgtgagcagc
    50461 tggggctgga tggcgaactg gtcagcgact gcgcggtgct gacgccgctt attcagacgc
    50521 tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg
    50581 tggttcatga gttcgcggca gcctgcggtt gtggtattga aatttcagaa gcggcactgc
    50641 cggttaaacc tgctgtgcgc ggcgtttgtg aactactggg actggacgcc cttaactttg
    50701 ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag
    50761 cgttacattc ccatccactg gggaaagacg cggcgctgat tggtgaagtg gtggaacgta
    50821 aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgattta ccacacgccg
    50881 aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt
    50941 ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat
    51001 ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc
    51061 tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct
    51121 attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcgtcgcg tgaaaaagac
    51181 acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg cagcattttg
    51241 tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg gccgcagctg
    51301 gccgcaggtg ggctataccc aaaatttggt cactattgcc tgatgccact ggcggcggaa
    51361 gggcatattt ttggtggctg tgaatttatt cgttatgacg atcgcccctg gagcgaaaaa
    51421 gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga acaaatccag
    51481 agccgcgtcg ttaacaatgt cgactatgag ttgttatgcc gggaacgcga taacttccgc
    51541 atcctggtcg ccatcaccaa cgcggtgctt tcccgcctgg atatggacga actggtcagc
    51601 gaagtcgcca aagaaatcca ttactatttc gacattgacg atatcagtat cgtcttacgc
    51661 agccaccgta aaaacaaact caacatctac tccactcact atcttgataa acaacatccc
    51721 gcccacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt
    51781 aaagagatgc tgctgatcaa tctccacgag cgggacgatt tagcccccta tgaacgcatg
    51841 ttgttcgaca cctggggcaa ccagattcaa accttgtgcc tgttaccgct gatgtctggc
    51901 gacaccatgc tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt taccactacc
    51961 aatctgaatt tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc
    52021 gcctatcagg aaatccatcg tctgaaagaa cggctggttg atgaaaacct cgccctgacc
    52081 gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag cgaagccatg
    52141 tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc
    52201 ggtgaaactg gcacgggtaa agagctgatt gcccgcgcga tccataatct cagtgggcgt
    52261 aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt gctggaaagc
    52321 gatctgtttg gtcatgagcg tggggctttt accggtgcca gcgcccagcg cattggtcgt
    52381 tttgaactgg cggataaaag ctctctgttc ctcgacgaag tgggcgatat gccactggag
    52441 ttacagccga agttgctgcg tgtattgcag gaacaggagt ttgaacgtct cggcagcaac
    52501 aaaatcattc agacggacgt acgtttaatt gccgcgacta accgcgatct gaaaaaaatg
    52561 gtcgccgacc gagagttccg tagcgatctc tattaccgcc tgaacgtatt cccgattcac
    52621 ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc ctttaccttc
    52681 aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac gctgcgcacc
    52741 ttgagcaaca tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgcg
    52801 gtattgctaa cacgcggtaa cgtgctgcag ctgtcattgc cagatattgt tttaccggaa
    52861 cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg gcgaagatga atatcagttg
    52921 attgttcgcg tgctgaaaga aaccaacggc gtggttgccg ggcctaaagg cgctgcgcaa
    52981 cgtctggggc tgaaacgcac gaccctgctg tcacggatga agcggctggg aattgataaa
    53041 tcggcattga tttaactgca aattgccgga cagatctgcc tgtccggcat actattcacg
    53101 aggttttttc ggacgatatt tttccggcag ttctggcacc ggacgcttgt catcgatgag
    53161 atgacgcacg gttaagatcg gatgacgcca cagcattctc ggcccggccc aacgcataat
    53221 ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc
    53281 tggtttctct tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa caaacaacgc
    53341 ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat
    53401 ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccacctca
    53461 ttaagatgta tttatattac atcttaatct taaagggcac tatgactcta aagaagaaag
    53521 gttcgccaac cgacgcaatt ttgcgtactt gcttcataag catcacgcaa aagctgcaaa
    53581 acagcatctt tcccggaacc agcatcaaga actcgccgtt cgcttcttcc cctgaaatga
    53641 ttaactccgg tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacaccc
    53701 catttaatat cagggaaccg gacataaccc catgagtgca atagaaaatt tcgacgccca
    53761 tacgcccatg atgcagcagt atctcaagct gaaagcccag catcccgaga tcctgctgtt
    53821 ttaccggatg ggtgattttt atgaactgtt ttatgacgac gcaaaacgcg cgtcgcaact
    53881 gctggatatt tcactgacca aacgcggtgc ttcggcggga gagccgatcc cgatggcggg
    53941 gattccctac catgcggtgg aaaactatct cgccaaactg gtgaatcagg gagagtccgt
    54001 tgccatctgc gaacaaattg gcgatccggc gaccagcaaa ggtccggttg agcgcaaagt
    54061 tgtgcgtatc gttacgccag gcaccatcag cgatgaagcc ctgttacagg agcgtcagga
    54121 caacctgctg gcggctatct ggcaggacag caaaggtttc ggctacgcga cgctggatat
    54181 cagctccggg cgttttcgcc tgagcgaacc ggccgaccgc gaaacgatgg cggcagagct
    54241 gcaacgcact aatccggcgg aactgctgta tgcagaagat ttcgccgaga tgtcgctgat
    54301 tgaaggccgt cgcggcctgc gccgtcgccc gctgtgggag tttgaaatcg acaccgcgcg
    54361 ccagcagttg aatctgcaat ttggcacccg cgatctggtc ggttttggcg tcgagaacgc
    54421 gccgcgcgga ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac
    54481 gaccctgccg catattcgtt ctatcaccat ggaacgtgag caggacagca tcattatgga
    54541 tgccgcgaca cgtcgtaacc tggaaattac tcagaacctg gcgggcggtg cggaaaatac
    54601 gctggcttct gtgctcgact gcaccgtcac gccgatgggt agtcgtatgc tgaaacgctg
    54661 gctgcatatg ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa ctattggcgc
    54721 attgcaggat ttcaccgccg agttgcagcc ggtactgcgt caggtcggcg acctggaacg
    54781 tattctggca cgtctggctt tacgaactgc tcgcccacgc gatctggccc gtatgcgcca
    54841 cgctttccag caactgccgg agctgcgtgc gcagttagaa actgtcgata gtgcaccggt
    54901 acaggcgcta cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc tggagcgagc
    54961 aatcatcgac acaccgccgg tgctggtacg cgacggtggt gttatcgcat cgggctataa
    55021 cgaagagctg gatgagtggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct
    55081 ggaagtccgc gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt
    55141 gcacggctac tacattcaaa tcagccgtgg gcaaagccat ctggcaccca tcaactacat
    55201 gcgtcgccag acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga
    55261 agataaagtt ctcacctcaa aaggcaaagc actggctctg gaaaaacagc tttatgaaga
    55321 actgttcgac ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc
    55381 ggaactcgac gtgctggtga acctggcgga acgggcctat accctgaact acacctgccc
    55441 gacctttatt gataaaccgg gcattcgcat taccgaaggc cgccatccgg tggttgaaca
    55501 ggtgctgaat gagccgttta tcgccaaccc gctgaatctg tcgccgcagc gtcgcatgtt
    55561 gatcatcacc ggtccgaaca tgggcggtaa aagtacctat atgcgccaga ccgcgttgat
    55621 tgcgctaatg gcctatatcg gcagctacgt accggcgcaa aaagtcgaga ttggcccgat
    55681 tgaccgtatc tttacccgcg taggcgcggc agatgacctg gcttccgggc gttcgacctt
    55741 tatggtggag atgaccgaaa ccgccaatat tttacataat gccaccgaat atagtctggt
    55801 attgatggat gagatcgggc gtggaacgtc cacttacgat ggtctgtcgc tggcgtgggc
    55861 gtgcgcggaa aatctggcga ataagattaa ggcattgacg ctgtttgcca cccactattt
    55921 cgagctgaca cagttaccgg agaaaatgga aggcgtcgcc aacgtgcatc tcgatgcact
    55981 ggagcacggc gacaccattg cctttatgca cagcgtgcag gatggcgcgg cgagcaaaag
    56041 ctacggcctg gcggttgctg ctctggcggg cgtaccgaaa gaggttatta agcgcgcacg
    56101 gcaaaaactg cgtgagctgg aaagaatttc gccgaacgcc gccgctacgc aagtggatgg
    56161 tacgcaaatg tctttgctgt ccgtaccaga agaaacttcg cctgcggtcg aggcactgga
    56221 aaacctggac ccggattcac tgactccgcg tcaggcgctg gagtggattt atcgcttgaa
    56281 gagtctggtg taataaaaat tcccgatagt cttttgctat cgggaatatt aacgacaact
    56341 gacgaatcaa ataaaaatac cctgtataat aggaaagctt attttacagg gtaaaaccat
    56401 gccatctaca cgctatcaaa aaatcaatgc ccatcactat cgccatatat gggtcgttgg
    56461 tgatattcat ggtgaatatc agttattaca atcccgctta catcaactct cttttttccc
    56521 cgaaaccgac ttacttattt ctgtcggcga taatattgat cgtgggccgg agagtcttga
    56581 cgtcctgcgc ctgctaaacc agccctggtt tatctccgtt aaaggcaacc acgaagcaat
    56641 ggcgctggat gcgttcgcga ccggcgatgg caatatgtgg cttgccagcg gtggtgactg
    56701 gtttttcgat ttaaatgatt cagagcaaaa agaagctaca gatctgttgc tgaaattcca
    56761 tcaccttcca catattattg aaatcactaa cgacaacata aaatatgtca tcgcacatgc
    56821 agattatccg gggagtgaat atctctttgg taaagaaata gcggagagcg aattactctg
    56881 gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa acggcgctga
    56941 ttattttata tttggacata tgatgtttga taacattcag acgttcgcta accagattta
    57001 tattgatacc ggatcgccga aaagtgggcg gctgtcattt tataaaataa ggtagtctca
    57061 tgcttcttct gtgaagcatg agtaaccaga tgttattgca ggccattatt catttttcgc
    57121 taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg gcaaaccgtc
    57181 gtctgtaata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg ccacctgacc
    57241 atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa tcgcccgttt
    57301 caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg acgatgcaga
    57361 aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt ccccgacaca
    57421 ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat tactgttgtc
    57481 gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact ccatgtgaat
    57541 cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac aacttcccgg
    57601 ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg ccagcgcagt
    57661 ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc ctccggacac
    57721 cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg tcatatgtga
    57781 cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag ctaccatctc
    57841 aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt gtcctgctga
    57901 cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag ttcacataaa
    57961 ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt ccagcgctta
    58021 tattcacaat atcaaacaaa atatcactta aattaacaag gagagcagat gaaaacggga
    58081 tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc agcactgtca
    58141 tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc ctgcgctacg
    58201 ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc cgaaaaactg
    58261 gacgctctgc tggtgctggt ggtcaatgcg gcccaggtta aacaggtgct gtttggtgaa
    58321 acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc cactatcgct
    58381 agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga aatgctggat
    58441 gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt catggcctcc
    58501 ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc cggaaaagtt
    58561 tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca ccagttgtta
    58621 gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg tgcggggatc
    58681 ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg gatgttcgaa
    58741 aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt cgatattttt
    58801 gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc gctgccattg
    58861 gcctcaacag cattgaatat gttcaccagc gccagtaacg caggttacgg gaaagaagac
    58921 gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa atcatgatca
    58981 agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt tttctggtgg
    59041 aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg ccggaagcaa
    59101 tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa gccacacagc
    59161 aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc tatttcaaat
    59221 actgctctac tttcgacagt acggcgaaag gtaatatcgg cccggttacc gatgcattaa
    59281 tggatgctct cgacacgccg tttacggtct tctctccggc cctgccggtc aacggacgta
    59341 cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc gggatgcgcc
    59401 atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa gcgcaatcca
    59461 cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggtgtggat gccgttcgtc
    59521 aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat gcgctgaccg
    59581 aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta acgggcggtt
    59641 ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac caggctcgcg
    59701 aagcagggca tccgctcgct gggcgcggcg tagtgctctc cggttcatgc tctcaaatga
    59761 ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt gatgtggcac
    59821 gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag tgggttctgg
    59881 gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac gcattggcag
    59941 caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact ctattttctc
    60001 aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca ggcggtgaga
    60061 cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc ccaaccattt
    60121 cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc cttaaatctg
    60181 gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta tcatgagcga
    60241 tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca gttcattctt
    60301 tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac ctgacgggaa
    60361 tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc ggctttccaa
    60421 agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg tgctctttca
    60481 tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc acagcacatg
    60541 gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc gtccgttcac
    60601 accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc gaccgggcga
    60661 taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt ttttactggc
    60721 aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca atatggaaga
    60781 gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc gttatctgac
    60841 cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca gctaatttat
    60901 ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga aaagccgggt
    60961 tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc caaaagcaac
    61021 tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat attaatgcgg
    61081 gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca gacatcgacc
    61141 tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg gcaggcgtcg
    61201 tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat ctccgctacg
    61261 ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc agccccgacg
    61321 ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt gaagaggttg
    61381 cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta gatggcaacc
    61441 tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt gccggactac
    61501 ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc cgcctgttcg
    61561 atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc ctcaccgaag
    61621 aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt acaacgtacc
    61681 catacatccc cccatctacg gatggggtag gttaactata actttcagac agggtttccc
    61741 atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct gttgctgctg
    61801 gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct gttagtcgca
    61861 cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg gatgggcggt
    61921 gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg tatgatcgaa
    61981 cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg tgacaaacga
    62041 actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt ctttgatgtc
    62101 ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa aatatcgcca
    62161 ctcaaatttg gcctgcctgt cgctggaatc atgctaactg ttcatgtggc ggtaccgccg
    62221 catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg gctaaccatc
    62281 atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc gaaaataatc
    62341 aatacgcgcc aatatgcgat gtcggtggaa gtactggaac agatgcaact ggctccggcc
    62401 agtgaggaag gcgcaacaaa attaagcgat aaaataaatc caccgggcgt cgcgctggtc
    62461 acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc cgcaacactg
    62521 atgccgcctt cgcatcccca gcttggtacg ctacagctga tcggctcacc aatggtagct
    62581 ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg ctggagctta
    62641 caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt gattttggtt
    62701 actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg caaagccctt
    62761 gccaatatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat tatttcactg
    62821 gctctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg cgggttactc
    62881 tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct ggcagcctgc
    62941 tttggtgggc ttggcgcgtc acatattaat gactcagggt tctggattgt gaccaaatat
    63001 ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac cattctcggt
    63061 tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta ataaaaaggt
    63121 ccgatgggca tcggaccttt tatttaacgc ttaccttcca ccagcagcgg cgggatgctc
    63181 ggcaccattg gcgcgtcatc aatatctttc tgcgtcatgc ggaacgcttc gggataatgt
    63241 tcgcggctgg tacggcgcag cggttcggta tcgcgccagg tataaaggca atgctggcac
    63301 tgatataccg tccagacatc tttcaccggc gatttcgcca tcacttcaat ctgttcatcg
    63361 gcacaacgtg gacaaatcat cttcttctcc ttatttacgt gcagccagca tagcggtcag
    63421 tttttcagcc caggctttgg tttccggtaa atccaccacc ggttggctgt agtgaccacg
    63481 gttgtccggg gcgacaggcg tagtggcgtc gataatcagc ttgtcggtga tccccgcagg
    63541 gcttgagcct ggatcgagtt ccagcacgga catattcggc aactgcacca aatcccctgc
    63601 cgggttcact ttcgaggaga gcgcccacat cacctgcggc aggttgaacg ggtcaacgtc
    63661 ttcatcgacc ataatcacca tcttcacgta gcccagacca tgcggcgtgg tcattgcgcg
    63721 caggcccacc gcgcgggcaa agccgccgta gcgttttttg gtggagataa tcgccagcag
    63781 gccatgggtg tacatggcgt ttaccgcctg cacttccggg aactcggctt tcagctgctg
    63841 atacagcggc acgcaggtgg ctggccccat caggtagtcg atttcggtcc acggcatacc
    63901 gaggtacagc gattcgaaaa tcggcctggt gcggtaagag actttatcga tgcgcaccac
    63961 ggtcatgtta cgcccgccgg agtagtgccc ggtaaactca ccgaacggcc cttcgatttc
    64021 gcgtttacgg ctttcgatga ccccttcgag gatcacttct gaaccccacg gcacatcaaa
    64081 accggtcaac ggggcggtgg cgatcgggta cgggctttca cgcagcgcgc ctgccatttc
    64141 gtactcggac tgatcatatt tcagcggcgt ggcccccatc agcgtgatga tcggatcgtt
    64201 accgagcgtg atcgcaatcg gcagatcttc accgcgctct tctgctttat gcagatgcag
    64261 ggcgatatcg tgcatcggca ccggttgcag gccgagctta cgcttgccct tcacttccat
    64321 gcggtagatg ccgacgttct gcttgccgaa gttatccggg tcgagcggat cgcgggaaac
    64381 cacgcacgct ttgtcgagat agaaaccgcc atcgccatcg tttaaacgaa acagcggcag
    64441 gatatcgaac aggttgatct cgtcgccatc aacggtgttc tgcgcccagg ctggattggc
    64501 gcggcgctcc ggggcaatcg ggaagttatc ccagcggcgg ataaactcat caatctgctt
    64561 tttaaccggg gcatttggcg gcaggccgag ggaaatcgcg tggttctgcc aggaaccgat
    64621 ggtgttcatc gccacgcggg catcggtaaa gccgcgaata ttatcaaacc acagcgcggg
    64681 cgcgccgtcg ccgatacgcc cggtggcgtt agctgctgct gccagatccg gctcggcgtt
    64741 cacttcttcg ctgattttca gtaactggcc gtggtcatca agctcctgta aaaagctgcg
    64801 taaatcatca aatgccatta ttcattctcc tgagaaaaat tccgggcctg cggcaatcct
    64861 tgccagcgcc tggcgtgggg atgttcgagg ccaaattgat ccagcacgcg ggctaccaca
    64921 tggtggacaa tgtcatctac cgtttcggga tggttataaa aggcaggcat cggcggcacc
    64981 atcgccacgc ccatgcgtga aagtgcgagc atattttcga gatggatggt gctaagcggc
    65041 atttcacgcg gcaccagcac cagtttgcgg ccttctttga gcacgacgtc cgccgcgcgc
    65101 cctaccaggc catcggcgta accagcgcgg ataccggcga gcgttttcat actgcacgga
    65161 ataacgatca tgccgtcggt acgaaaagaa ccggatgaga tgatcgccgc ctgatccgcc
    65221 gggttatggc tgaagtctgc gagggcagca acatcgcgag cgctgtaagg cgtttccagt
    65281 tcaatggtgg ttttcgccca cttcgacatc accagatgag tctcgacatt cggcatctcc
    65341 cgcagcgctt gcagtaatgc cacaccaaga ggcgcaccgg tagcccctgt catcccgacg
    65401 atcagtttca ttgcttattc tcttaattgt tcgtatacga acgttattat taacatactc
    65461 cttcatatcc acagcggcaa ggattgaaaa gtaacaacat cttcttcgtc actaaaaaac
    65521 cgctatgata gcgacagaga attagcccgg agtttacatg gcgttacgaa ataaagcgtt
    65581 ccatcagtta cgacagcttt ttcagcagca cacggctcgc tggcagcacg agttacctga
    65641 cctgaccaaa ccacagtacg cggtgatgcg cgccattgct gataaacctg gtatcgaaca
    65701 ggtggcactg atagaagcag cagtcagcac caaagcaacg ctagcagaaa tgttggcaag
    65761 aatggaaaat cgtggcttag tcagacgtga acatgactcc gctgacaagc ggcgacgctt
    65821 tgtctggctg actgccgaag gagaaaaggt acttgctgcg gcgataccta ttggtgacag
    65881 cgtggatgag gaatttttgg ggcgtttgag tggagaagag caggagctgt ttatgcagct
    65941 ggtgcgcaag atgatgaacg catagggggc aaatttccgg atgcgacgct atcgcgtctt
    66001 atccagacta caaatacacc gactcaccaa ccgaaagaaa aaaggccagc ctcgcttgag
    66061 actggccttt ctgacagatg cttacttact cgcggaacag cgcttcgata ttcagcccct
    66121 gcgtttgtag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt
    66181 cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttcg taccccagca
    66241 aaccgaatcg acgtgccagc acttcacgct gtttggcgtt cagctcgaac agccatttga
    66301 cgatgctctg cttcatatca tcatcttgcg tggtatcttc cggaccgttc tctttttcat
    66361 cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg
    66421 taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct
    66481 ctgcgatctc ttccgcactt ggttcatggt ccagcttatg ggacaactca cgtgcggttc
    66541 gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt
    66601 tcataatcgc ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtc gagaagcgga
    66661 aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaggttg ccctcttcga
    66721 taaggtccag caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac
    66781 gcaagttact ctcgatcatc cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg
    66841 caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa
    66901 gctgagtcgc gtccaacaca cgctgtgtgg ctccctgcga taacagttcc tcttcggcca
    66961 aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt
    67021 tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa
    67081 ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat
    67141 ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa
    67201 atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt
    67261 cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc
    67321 atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac cggcataaac
    67381 aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga tatcaatccc
    67441 cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg
    67501 ccaggtggag ataggcgtac tggttgatgt actgctgaca gttggctcgg ttgtgcttgc
    67561 tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagct ggcttgttgt tcggcaacat
    67621 tttgttagca ctctgttcac ccgaagattc agaatacgta attgtcggtt gcgacgcaac
    67681 agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg cgtcggcctg
    67741 ggtaatggca tttccgccag tgattggcgt accggaagca ttacccacct gcaaggtctg
    67801 accaacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc
    67861 gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg tataggtact
    67921 gccgctataa ctgcctttcg gaatgttccc atactgacgg ttatagacga tgcgtccgtt
    67981 ttccatctgt accggctgct gagctactgg ctgcactggc tggatttgcg gttgttgagt
    68041 agcctgaatt tgtggctgct ttaccggctg aatttgcggt tgctgggctg tagacgtcgt
    68101 ccccattttc ggcggcggcg taatcaacat accagaattg gtatttgcag gcgcattgcc
    68161 attaacggag ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg ccagccatag
    68221 cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc ccgcgctcat
    68281 ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca aggcacccta
    68341 ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg gcaaaataac caggaaaaat
    68401 ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca aagcgcacgg
    68461 cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc aaatactggt
    68521 gctcctcccc tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc attagcgcag
    68581 ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgcg cgtgcctgcc
    68641 aaccttgcca tccatcgcca tgacgggttg aaacattatg taaatcaaga tttttcaggc
    68701 ggcgacgcgc ctgccactgc aaacctttaa tccgttcgac cgagcaaaca tgctggacaa
    68761 gatgcgccag gattgccgtt tgatatcccg aaccggtgcc aatttccagc acccgcgact
    68821 gcggcgtcag ctcgagtaat tcggtcattc gcgccaccat atacggctgc gaaattgtct
    68881 gcccctgacc tatcggcaag gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa
    68941 cgaatttttc acgcggcacg gcagcaagtg cattcagcac ctgctcatcc tgaatacctt
    69001 gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg tgccaactcc
    69061 cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg
    69121 cagcggcgtg atggagacat agccctcatc taccgcagca aaatcggtcc ccggaccggc
    69181 atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg
    69241 cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat
    69301 ttggtccagc ggcaaatccg gtacatttat attaagaata cgccctgttc gcagcggttc
    69361 tttgcacagt gcacgcagaa tagaacaggt aactgccgca gcggtgtcgt aatgtttatg
    69421 cccgtctagc gagacggcaa gcgccggaaa acctaaatgg cgaccttcca tcgcggcggc
    69481 taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa tcccggacac
    69541 aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacgc aatcggtcgg
    69601 ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca gggaggattc
    69661 cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc
    69721 agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc
    69781 attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg acaacgctgg
    69841 ttgcaaaact acccgccgga agccagaaac ggatctctac ggtgacgtca tcccaccaat
    69901 tccagcttaa ttgttgcgga tacagcagca tcgctctgcg cgcggcttca actttttcgc
    69961 gcaccagtaa agcttgtaat tcagtttctg cggcgacagc tgcttgttcg aatgccagcg
    70021 cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc atcagctctt
    70081 tatcgttgac gcgacgctgt aattccgcca gttcttcggt ggttgcgaca aaccagctac
    70141 cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct gcttttttga
    70201 ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac caaaaactcc
    70261 gtttattgcg atcgcgcacc ggagtattgg tttgcgccca gcgctgcgcc ccctgcaagt
    70321 tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt cggtacacct tttacgcaaa
    70381 tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgcaga accagggtaa
    70441 aggcgttacc tttcagcgcg cctaaacgca gcttgcgctt gtgccgcgca tactccagca
    70501 cctggcagcc ttccagttga aaggcgctca gatcgggcat ttccttgcct ggcacgcgag
    70561 cgcataacca ctgttccgta acagcatgtt tgtctttttg cccagcgaag ctgacttcac
    70621 gggcatgaat tttcaggaat ttcgccagtg catccgccac aaaacgggta ttgcagccgt
    70681 ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag cccaaatctt
    70741 ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc ggtttaccgt
    70801 ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta gcgccaccgc
    70861 ttcacaggca atcccttccc cacgtccggt aaatccaagt ttttccgtag tagtggcttt
    70921 cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc gcatttgtgg
    70981 aatgtgcggc aacatcttcg gtgcctgagc gatgatagtg acatcgacgt tgcccagggt
    71041 ataacccttc gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc
    71101 tttaaatgcc ggatcggtat ccgggaacag cttgccgata tcccccagcg ccgccgcgcc
    71161 aagcaatgca tcggtcaacg catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc
    71221 tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc caaaggcatg
    71281 tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt tcgggtgagg
    71341 taaaactcgg ccagtgccaa atcttccggg cgcgtgactt taatgttatc cgcacggcct
    71401 tcgaccaact gaggatggaa tccgcaatat tccagcgccg aggcttcgtc ggtaatagtc
    71461 gcgccttcat ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc
    71521 ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc atttttgccc
    71581 ggttcggcac gtttcatcgt atcgcgcact ggtgcggcta ggatccctcc cgtgcggctg
    71641 gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat gcagacaagg acgagcggcg
    71701 tcatgcacca atacccactg cgcgtcgcca gcggctttca aacctgccag cacggaatcg
    71761 gcacgctcat caccgccatc tacaacggtg atttgcggat gattcgccag aggaagttgt
    71821 gcaaaacggc tatcgccagg acttatggca atgacgacac gtttcacccg gggatgcgcc
    71881 agcagcgcat gcaccgagtg ttcaagaatg gtttgattac cgattgagag atattgctta
    71941 ggacattccg tttgcattcg acggccaaat ccggccgccg gaaccacggc gcaaacatcc
    72001 aaatgagtgg ttgccatgtt aattcccggg ctgatttatc gattgttttg ccccgcagac
    72061 tgtgcgcgct tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg cctggtcatg
    72121 ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag atcgtcaatt
    72181 tcggcaaaaa gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc
    72241 acatcatcat tgacgcgggt atagtcatgt ataccgttct taccgaacca cagcgaatac
    72301 tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct gccccctgaa
    72361 aaacggcatc atcatcccat gcatccgaag acgactctac atcctctgtt ggggataccg
    72421 cgacaacgcg ggcaaatgta ccacatttgt ccattgttac gtatacccag ggcgtgcaga
    72481 acataatctc attattagtt acggtttgaa ttatgaacag aggagacggg aaagtacaaa
    72541 ttagcccagt agccacataa acagtgcgcc aaacataatg cctactgtca ccagggtgaa
    72601 aacaatactg tagcgtagct ttccgtccat caatgaatgc agcgcaatcc ccaccactac
    72661 cgcgacgggc atcagcgcca gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt
    72721 agagccataa atcaacatcg gcatcgccag cgcaaataac caggagataa aaccgaccac
    72781 ggcaccaggc agtgaccatg tggtttcttc atcctcagta aggctgtcgt tatttgttag
    72841 tgtaatgtta tggctattac gcatatttga tcctgttact ttgacgaacc gggcatggaa
    72901 acccggtggt gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct
    72961 gtaccaaatt tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggcgctt
    73021 cgtaaacaga atctattccc gtaaagttgc gcagttcacc ggcacgcgct ttcttatata
    73081 agccttttgg atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa
    73141 agcgcccttc tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa
    73201 atgcggtcag cactaccagt ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac
    73261 ggatattttc tttacgatcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa
    73321 cattatcgcc atccagcaga tacgtactga cgccgagttt atgtaacgcc tcctccagcg
    73381 ccccggcgac cgttgattta ccggacccgg agaggccggt aaaccacagc actacaccac
    73441 gatgaccgtg gtgtagctcg cgttgttgca cagtgaccgg atggctatgc cagacgacgt
    73501 tttcgtcatg cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa
    73561 gtggcgacga accagagcat tcaattccag ttcgaatgca ctgaattcag atggcgcagc
    73621 agttgcctgg ctaactggct cgtgcaccat accggcaccc acggtcacat tgctcaggcg
    73681 atcgataaaa atcagcccac ccgtcaccgg attttgttga taacgatcta acaccagcgg
    73741 ctcgtcaaaa gtgagatcca cgaggccgat cccattcagt ggcaggtttt caacttcacg
    73801 ctgggtaagg ttattaatat caacctgata gcgaatgcca tcaacacgcg cgcgcgtctt
    73861 cttaccggca attttgatgt cgtaactctg ccctggagaa agcggctgtt ccgccatcca
    73921 taccacatcc accgacgcgc tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag
    73981 atcgccacgg ctgatgtcga tctcatccgt cagcactagg gtgatcgctt ctccggcaaa
    74041 ggcttcttcg cgatcaccat caaaagtcac gatccgcgcg acgtttgatt ccacaccaga
    74101 aggcagcact tttacacgtt gcccgacttc cacgcgaccg gatgccagcg ttccggcgta
    74161 accacgaaaa tcgagattcg ggcgattaac gtactgcacc gggaagcgca ttggctgagc
    74221 atccaccact cgctggatct ccacggtttc cagcacttcg agcagtgtcg gaccgctgta
    74281 ccacggcata ctttcacttt gcgaagccac gttgtcgcct tccagcgcag agagcggcac
    74341 aaagcgaata tccagattgc caggtaactg ttcggcgaaa gtcaggtagt ctttacgaat
    74401 gcgggtgaat gtctcttcac tgtaatccac cagatccatt ttgttgatcg ctacgaccag
    74461 atgtttgatc cccagcagcg tggagataaa actgtgacga cgggtttgat cgagcacgcc
    74521 tttacgggca tcgatcagta agatcgccag ctcacacgtt gaggcgccgg tcgccatatt
    74581 gcgggtatac tgctcgtgcc cgggggtgtc ggcgataata aatttacgct tctcggtaga
    74641 gaaatagcgg taggccacat caatagtgat gccctgttcg cgctcagctt gcaggccgtc
    74701 caccagcaga gccagatcca gtttttcgcc ctgggtgccg tgacgcttac tgtcgttatg
    74761 cagcgatgag agctgatctt cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag
    74821 ggtacttttg ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg
    74881 ttgcgcaatc atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtgcggt
    74941 gttcatctta aaaatacccc tgacgttttt tcagctccat agaccccgcc tggtcgcggt
    75001 caatcacgcg gccctgacgt tcactggtgg tagaaaccag catctcttcg atgatctccg
    75061 gcagtgtttg tgcatttgac tccaccgcac cggtcagcgg ccagcagccc agcgtacgga
    75121 aacgcaccat ccgtttttta atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa
    75181 tcatcatcaa cataccgtcg cgttccagaa ccggacgttc cgcagcgaga tacagcggca
    75241 caatgtcgat attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga
    75301 gcgggaaaac gcggatgctt tcgcctttgt taatctgccc gttatagttg tgccacagct
    75361 ccgggcgctg atttttcggg tcccagcgat ggaagcgatc acggaaagag tagatacgct
    75421 ctttagcacg ggatttctct tcgtcgcggc gcgcaccacc gaaggcggca tcaaaaccgt
    75481 atttattcag cgcctgcttc aggccttcgg tcttcataat atcggtatgt ttcgcgctgc
    75541 cgtgcacgaa tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct
    75601 cgcagccgta ggctttagca gtacgatcgc ggaactcata catctcgcgg aatttccagc
    75661 cggtatcgac atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca
    75721 gatgcagcat gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt
    75781 ctgccgccac ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aggtgagtaa
    75841 gtcgtatttg atccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt
    75901 tcgactatag ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta
    75961 acggaataac gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc
    76021 gtttaggaag atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa
    76081 aatttgcatg gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc
    76141 ctggcgctcg gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat
    76201 tttgctaata cccaggcacg acatattgct actttctttc cgggacgcat gaccggaact
    76261 ccagcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc
    76321 agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagaac
    76381 tggcataacg tgacaggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag
    76441 atcatcatta tggcgcatct ggatacttac gccccgctga gcgatgctga cgccgatgcc
    76501 aatctcggcg ggctgacgtt acaaggaatg gatgataacg ccgcaggttt aggtgtcatg
    76561 ctggaattgg cagaacgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg
    76621 accagcggcg aagaggaagg gaaattaggc gctgagaatt tactcaagcg gatgagtgac
    76681 accgaaaaga aaaatacgct gctggtgatt aatctcgata acttaattgt tggcgataaa
    76741 ttgtatttca acagcggtgt aaaaacccct gaagcagtaa ggaaattaac gcgcgacagg
    76801 gcgctggcaa ttgcgcgcag tcacggaata gccgcaacga ccaatccggg tttgaataaa
    76861 aattatccga aaggcactgg gtgttgtaat gacgcagaaa tattcgacaa agcgggcatt
    76921 gctgtacttt cggtggaagc gactaactgg aatcttggga ataaggatgg ttatcagcaa
    76981 cgcgcaaaaa cagccgcatt ccctgcggga aatagctggc atgacgtaag actggataat
    77041 caacagcata ttgataaggc acttcctgga agaatagaac gtcgctgccg tgacgttatg
    77101 cggataatgc taccgttggt gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg
    77161 gagctgggag ttctaccgca ggggcgggga attctaagtg atatccatca tcgcatccag
    77221 tgcgcccggt ttatccccgc tggcgcgggg aacacaaaac caaacttctc cataaattcc
    77281 atagccgcgg tttatccccg ctggcgcggg gaacactagt taacttttag acagaatatc
    77341 cgtgtacccg gtttatcccc gctggcgcgg ggaacacggt tttacaagct taaatgattt
    77401 taaattttgc ggtttatccc cgctggcgcg gggaacacaa ctggattctg ctctaattat
    77461 tacatcatcg cggtttatcc ccgctggcgc ggggaacacg gaaaccgccc cggcggtggt
    77521 tagctgtttg ccggtttatc cccgctggcg cggggaacac ttattttatt cagctcgcac
    77581 aggtatcgtc gccggtttat ccccgctggc gcggggaaca cgctcgtctg cgtattcgta
    77641 gcagtcaaca atacggttta tccccgctgg cgcggggaac actggtttgc tatctgatgg
    77701 aatttgaacg gatgcggttt atccccgctg gcgcggggaa cacgacgtaa caccggatcc
    77761 ggcgcgtact gcattaggtt tatccccgct ggcgcgggga acacactaag catacatatc
    77821 tgtttttaaa caaatttatt ccacatcaac aatctaccaa ctaaattcaa acatttcctt
    77881 atttttaaag aacgtctaac ttattgatta tcaacaggaa gaaaagaaac caaacgtaac
    77941 ccatccaaat ccaccggaat acgtctgttt ttgccccagg tctgaaactc aaaaccagac
    78001 tcagtatttg tcgcccaggc catgaccaca tttccacaac ctgccagttg ggtaatttgc
    78061 tgccagatca tctcccgaat acgttttgat gtatcaccaa catacacacc ggcacgcact
    78121 tccagtagcc agattgcgag ccgtccacgt aagcgcggcg ggacattttc tgtaacaacc
    78181 acgaccatgc tcatcagccg cgtcctcgat gaccgctatc gcccaacgtt tcaggttcgg
    78241 ggatggctgg cggtaacata tccggcgcag gttgtggcgg ttcaatttca cctgcggcaa
    78301 ggacttcctc aattaacggt attaatttgc ccgttaactt agtgctacgg aaaatatcac
    78361 gacaggctaa tctgacttct ttatcaggtt ctgcgggttg cctcgccgct atttcaaatg
    78421 cctttggtac aaccgaatca aatttaatga tatcggctat gtcataaaca aatgaaagcg
    78481 gtttgccact atggataaac ccaatagcgg gcgcatatcc cgcggctaat actgccgctt
    78541 cagatatacc gtacagacat gatgtagcag cactgatgca gcgattcaca acatcgcctt
    78601 tttcccagtc tttaggatcg tatttgcgac cattccattt cacaccatat tgtttcgcca
    78661 gtaatgcata cgtctggcga acgcgagatc cctcaattcc acgtagctgc tccactgaac
    78721 ggcgagctgg cggtggctca cgaaaacgta attcatacat tttgcgcacc accttcaggc
    78781 gtagatcttc cgttaaagcc agctttgcct ggtagagtaa cttatcagca cgcgccccac
    78841 cgggttgtcc ggaagagtag acgcgaacac ccgcttcacc gacccagacc agtagcgtcc
    78901 ccaccgtggc ggccagatgc accgccgcgt gggaaactct cgttcccggt tcgagcataa
    78961 tgcaggcgac cgatcccacc ggaatgtgcg tgcggatccc ggttttgttg atcagcacga
    79021 aagcaccgtc cagtacgtcg atttgaccgt actggaggaa gatcatggag gtgcgatctt
    79081 ttaacgggat cggactcagt ggtacaaacg tcacgcctct gctccgggtt tgatcagcat
    79141 cagaccacag ccgaacgccc ggcttttgcc atacccctga ctgagacgtt gcagaaataa
    79201 cccgggatcg gtgaccgtaa gcatccccgt ataatccacg ctactgaact ggatcagttg
    79261 ccgggtgttt tcccgccgga gttgttgttg tcggtaggca tcaaccgagg tatccagtag
    79321 tgtaaaacca cttctttccc cctgcgctgc cagccagtcc agcgccgcct gttgttgatg
    79381 caaccagata tcacgccctt ccgcctgccc cttcacctgc cgtttcgcct ccatcagcag
    79441 atcgtggcgc ttgcccgctt tacagattgt tggattcgcc cgcaggttga aacaaagaga
    79501 ttgtccggta cgcagctcgg acacaaatgg tcggcattcg atggtgaaag tttcactttc
    79561 cgccgggcgt tcctgcgaga ggacaaaaaa gcgaaacgcg ccctggagtt cttcccgacg
    79621 ataaagaaat tgcctttctt tgccgccagg gaagagatcc cacagccact gatgcatcac
    79681 atattcaccg cgatccacca gatgcagcaa ctgcgctggg gaaagctggc cggtatgtaa
    79741 ggttattctt gagaggtaca tggttcctcc ttgctgagcc acggcccctg attgacggtg
    79801 cgttccccaa acaaccactg ctgacgattt aaaggaacat cccgacggcg taatattttg
    79861 ctcgcgacca ggccgtcgtg ttccccttcc caccagcatt catcctgaag ttttgggagt
    79921 gagactttca gtttgcggaa atgatcctga tactgttggt atgcgttacg taaggtatcc
    79981 gacgcgttgc cttcgagtag taacggcgca agtggtaacg ccagcggatg actttttcgc
    80041 cccagataaa gcggaaaaac cggatggcgt aaaccgtcct gcaactgttc gaggctgtaa
    80101 ggcgcatcgg gggttgttgc caccgccacc atccaccagg catcggtgta gtagtcgcgc
    80161 cgggagataa tcgcgctcaa aagctcaggg gcgctcaact cttcgcgacg gctgaaataa
    80221 cgtgctttac gcacctcttt tggcatctgg accgtgtgat aatcccgtgc ccagcgcggg
    80281 ttacggctgg cgcaaaccac cagtgaatag tggcgattaa acgcgtttaa gcgttcggta
    80341 tcatcacgcc ggatccctac cccggcagcc agcagcccca gcaatgctga gcgcgaaggc
    80401 agttcatggg tatgacgcac ttcgccgggg gcatcgacgc cccaggatgc catcggccca
    80461 tgaagctgaa aaatcagata ttggctcata agccgcccct tacgcgcaga taaagtccag
    80521 cacgtccttc atgcttccct gtttgttcat cacgtcaaaa cttgcgtaat cggtcttctg
    80581 ttcgtagacc gtattcatat tttcgcgaag agttgtaata cgctgcaccg ccacctctaa
    80641 ctgacgggtg ccattaatgg gttcatagaa agctgccgcc agagaacgcg gttgatcggt
    80701 gcctttttct gccagcgccc aggaggcgta ggcacggctg gcaaagctgt tctgtttgcc
    80761 ggttggggag actttaagtg cggcttccgt aaaggcacgt atggtctgat tagctaacgc
    80821 ttcgtcaccg ccaaggtttt cgaccagcag atctttatca atgcagatat aggtgtagaa
    80881 cagtgcagaa ccgaatccgg tttcaccaag atgcccggca ccggcatctt cagaagcctg
    80941 gcgcaaatca tcaacggcgg tgaaaaaatc atcttcgaca atcgtttcac tgacaccaaa
    81001 tgcgtgcgct acctggcagg cggcttcaac attaaactcg ggtttattcg ccagcatacg
    81061 accaaacatg gcaatatcta ccgccatacg gtctttgcgt aacaacgcga gatcttcctc
    81121 ttttggcgcg cgcttttctt ctgcaagctg atgggccagc gcttttacgg cgtcaaattc
    81181 tgccgggctg atatggacta actgttcagt ttcggcgtta gtgagcggat cttttggttt
    81241 tttgtcattt ttagctttcc caagataatc agcaattttt gccgaccatt cgatggcttt
    81301 tttatcttca atccctttct caatcaggat agttgccgcc tcacgcgcaa tacgcccact
    81361 gcgaatacca atatggcccg ccagtgcctg ttcaaaaagt gcagaagtgc gccacgcacg
    81421 tttcagactt tgcgaggaaa cgcgcagacg cgttgctcca cccagcacca cagtttttgg
    81481 cgcaccggta tcatcacggt taaggttggc agcagggtaa gcggttaaca aatgaagctg
    81541 aataaatgtt gtcatgagat agtcctttat tgattttgtt cgttatcggc gtcgccagcc
    81601 tggtaatatt ccagcgccca gcggatacgg ataaattcgg ttggccgctg ctggcggcgg
    81661 tgatgattga gcaggtcgtc gctctcctgg caccaacgga agaccccctc tgccagagag
    81721 tcaagattga cggaaccgtt taataatctg actgcgcggc gtaactggcg cagtaattca
    81781 tccggtgttt ttactgccga cagacgagta aaacgcccct ttgacattac cgccgccagc
    81841 tgcgcggcaa aaggctgccg ttcatcaata gcttttacat tggcgctaac tgcggcgacc
    81901 agcgccagtg cggtaaaacg ccactcttgt gcttcaattt tccacaaagt gtgggtctgc
    81961 atcagcagag aacgaaaccc ttctgacaag caaacatcat taaccgttgt gctacgccgc
    82021 agacttgcac gattgccgcg tttagcctgc aactcttcgc gccatttacg cagcgtggct
    82081 ttgtgctcct cttttacaat actcattcag cagcctcctg ctttttctcc ctggcggctt
    82141 tagcactttg cttctccgcc gatgttgtga aatatttctt gcgcgcggtc ataacgcgtt
    82201 tcaaatcaac aggctcatat ggattggtga atacacgctc gtcaaaatcc tgacgcgcga
    82261 ataaccaaat ttccttttgc catttgccga gtaattcgtc cgcatcctga ccttcttcaa
    82321 tttggcgcac taacctcagg aagcgatgct gagttttgtt ccagaagtcg atatccacaa
    82381 aactgaaatc accccgtgca ccttttggat cggagaacca cgcttctttc aatgcacttc
    82441 gtaacagact tagaatccgt gaagccgttt gcgcagccag acgcagtttc ggtatctggc
    82501 cttctttttt cttgagcagc agcgggaaat ggtgttcgta ccaacagcgc gctttcatgt
    82561 tgtcgaaatc atagccaaat ccccacaggc ctacttttgc ctgtttcaga ctgctggcat
    82621 taaagagttt caccaccagc gcgggaagtt ccgtattgtt ttctgattta cccgtttcga
    82681 taaggcctaa ccagtcgcgc cagattaaac cgcccggttg tggtttgacc gagtaaaact
    82741 cgccaccctc tttaagtggt atacggtaag gcgttaaggg atgctgccac atggcataat
    82801 tcgcaccgta atttttggtc gtcatcaggc tcaggagcgc gtcgctctgt tcaccgcaaa
    82861 tatcgcagtt gccgactgtc gtggtattaa aatcaatacg aatacggcgc ggcattcccc
    82921 agtaagcctg gagtttattg acctgatcat gggttaccac cgcaccggcc agttcgctgg
    82981 ttcgcgtcgg gccaagccag gggaaaacca gatcgtcaaa ttttttgggt agcggtaagt
    83041 cggcttcatc ctgcggcatc acattgggcc acagtttgcg ccacaagggc gtttgttgat
    83101 tgccttgata ttcctgtaat tcaatcagag tggtcatcgg cccaccgccg cgcaaaccgg
    83161 tgcgatagcc tttgccgcct gatggcgcat ttaactgtag ggagaacaga gctaacgcgg
    83221 aacaatgagg gcatacgtgt tcagtcacgc cacgcttaat aaagtggtct ttattaaact
    83281 tcgttgtttg agcgccggga atctcaggca gtagcgaagc gacctgaact ttatctccct
    83341 tgagcgcctc gaaatcctgc ataaatgaag gggaatctgg cccaaactgg aaagcatgtt
    83401 ctaatgactg caatgcttcc cgtagctttt cagcttccag tccgtcttcc cagatatcat
    83461 cccaacgacg atgatctttt ggcgcgaagc tggtttgtag tagccccagc aaaaactgcc
    83521 ataccgcacc ctggagatct gcccgcggcg cagcgatatc gacaacattt tcatcagcca
    83581 gatcgactgg tgccagcttg cctgtcgttc cgtctttaaa acgaacaggc aaccacgggg
    83641 ttgtcagaag tgaaaacgag ttcatacgca tcctctcgta aaaaagacca tcccaggtcg
    83701 gttgtccatc tttccatcct tttcggtgta tcaactattc agagagtcct gtcgcagaca
    83761 tctcaaccaa ccttgccaat caatccttcc gtagccgaat acccgcaact ttcgtcatcg
    83821 ttgactaaaa tcacgtttgc catctccgga tcttgtcgct gttcaatgca ccacagcctg
    83881 aacgcctctc cctccagtaa agaaaactca tcccgatgtt ttttccacca gcttcggcgc
    83941 actctgacaa cgctcatttc ccatgcgtga gcaccggtgg cataaggctt caccacaccg
    84001 tcaatacagg tagccagcca cagggaaaca gactcctcag ccagacgtgt cgacagtttt
    84061 tccgggaggt aatcgttgat attggcggca tagccgggcc tgaagttcag cacaaacttt
    84121 ttagccattg cgcgatcgca gtaatatttg cccacttgct cctgctcgct gcgggcaaat
    84181 ccttccggca ttaccacgtc ctcaccgtag acggattcaa taagaagccg ggctgcgtgt
    84241 ggcatctgaa tagcaccttg ctcacgcagc acacgctgcg tcagccagat tcgcccatga
    84301 tcgggataga caaatgcact gttacgcatg gcactgccaa accattcgtc gccaggagag
    84361 tcgtcccaga cgggagccag aatcagcaat tcaggggggg aacgctcgtc ctttccgtca
    84421 cgctttaact gaccattaat atcgcggata tgccgctgta atcgtcctgc tcgctgaatc
    84481 agcaaatcaa caggggccag gtcggagatc atttcgtcca ggtcaatatc cacgctttgc
    84541 tcaattactt gtgtacaaac aatgacttgc gacgctctct gtaaactaca atatttacca
    84601 aagcgtgcca gtgtctccgt ttcaattcgc tggcgatcgc taaaagcaaa gcggctatga
    84661 aaaagtgaaa ggctggaagc aggaatgacg ccgcgggcaa gcagctgacg atgaacctta
    84721 atagcgtcat cgacagaatt ccggatccag gcgatgcatt tcccctgact taccgccgat
    84781 tcgatacgcg caatacactc ttgttcacta tgaagccaac ccacgctgac gctacgctca
    84841 acgtctttgc gcgtcgctac ccggtgtgag ttcacatcgg atttcgtgac atgcgtcagc
    84901 caggggtaat catccttttc caggaacgga gcttcttgct ggccctctgt accccgcgca
    84961 aaggcagcga cgagtttgtc gcgctgctgt tgagataacg tggcagaaag caaaataacg
    85021 ctgtttccgc cacgcgcctg ccgctcgatc aacccttcaa gaatgcacga catgtaagcg
    85081 tcacaggcgt ggatctcatc agccagcagg attttgttac tcagccccag aagccgcaga
    85141 ttattatgtt tgaacggcat caccgccatc atcgcctgat ccagcgtgcc gacgccaatt
    85201 tcagccagca acgccttctt gttactgtcg gcaaaccagg ccgcacatcc ctgactgaac
    85261 gtttgttcat ccggttcttc tgacccgact aaatcaccag accagagtga ttcattaaag
    85321 cggtccatta atgtgcgggc actatgtgcc agcaccaggc tggggcggga ttctggcgaa
    85381 tagaaagcaa gccaggtttt gaccagccga tcgtacatgg cattggccgt tgccattgtt
    85441 ggcagaccaa aaaacagccc ctgagctttc cccgcagcta tcaacctgtg cgccaggata
    85501 agcgccgctt ctgttttacc tgcgccagtc acatcttcca gaataaataa ctgcggtcct
    85561 tgctggctga tatccagatc cagcaccttt tgctgtaacg gtgtcggatt ctcaataaaa
    85621 ggaaacagcg tattaattcc gttgaaaggc gcgacttttg cttttagagg aaagacggtt
    85681 aaggcttttt gagcctgaat taaagttttc tgccagtaac ctttaatatc cattgggtgt
    85741 gcgacgcgtg gaaaaaatcg cgttgacgaa cccgtccagt ctgcgagcac gacggttgca
    85801 gagatatacc aggaaaggtg ttttataagt tcaatgccct catcatcatc ccagaatgcg
    85861 gggatctcta tgagcggaaa cagtggtttg atttcaagga gaaaatctcg tgctgcagct
    85921 ttgtcttcag gcagaaaatt atccaattca tccatacgat cagggggctg accatgatgc
    85981 cctgtcgtca cgggcatcca catttctatc actcgcctaa gtttgcgcgg agagagcgaa
    86041 gatgaaggaa gtaactcctg acattcactt aaataatgat tccacaacca gtagcccagc
    86101 gttgaatgag agattttttc gtaatttttt ctggcacctt cctgaatctt gagttcaggg
    86161 ggcaggtaaa gttgttgaaa agagcgggca aattttccaa tatcgtgcca gcacagcaac
    86221 caggcgaaaa attgagccgc ctgttccttg tcagaaatcc ctaattgacg aaaataatca
    86281 gccagcccga agcaatttct tttaaccatt aaatagccca tcgcggctac atccagcgaa
    86341 tgccagcaaa gaaggtgata gtcgtcgccc ccgtctttct cgccgcgtcg ggtttttccc
    86401 cagaaatcaa aaaaagtcac aatattttta tccttcagta aacttaaagg atatttacgc
    86461 ata
//
LOCUS       DAZFFI010000007        84625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFFI010000007 DAZFFI010000000
VERSION     DAZFFI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.007"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            231..857
                     /gene="ydhY"
                     /locus_tag="ACHJ12_000640"
     CDS             231..857
                     /gene="ydhY"
                     /locus_tag="ACHJ12_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4988475.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            878..2980
                     /gene="ydhV"
                     /locus_tag="ACHJ12_000641"
     CDS             878..2980
                     /gene="ydhV"
                     /locus_tag="ACHJ12_000641"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4988476.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2993..3631
                     /gene="ydhW"
                     /locus_tag="ACHJ12_000642"
     CDS             2993..3631
                     /gene="ydhW"
                     /locus_tag="ACHJ12_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4988477.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3695..4363
                     /gene="ydhX"
                     /locus_tag="ACHJ12_000643"
     CDS             3695..4363
                     /gene="ydhX"
                     /locus_tag="ACHJ12_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4988478.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4360..5145
                     /gene="phsC"
                     /locus_tag="ACHJ12_000644"
     CDS             4360..5145
                     /gene="phsC"
                     /locus_tag="ACHJ12_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4988479.1"
                     /translation="MNPSQYAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5149..5961
                     /gene="ydhT"
                     /locus_tag="ACHJ12_000645"
     CDS             5149..5961
                     /gene="ydhT"
                     /locus_tag="ACHJ12_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4988480.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5973..7577)
                     /gene="ydhS"
                     /locus_tag="ACHJ12_000646"
     CDS             complement(5973..7577)
                     /gene="ydhS"
                     /locus_tag="ACHJ12_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4988481.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7703..8008)
                     /gene="ydhR"
                     /locus_tag="ACHJ12_000647"
     CDS             complement(7703..8008)
                     /gene="ydhR"
                     /locus_tag="ACHJ12_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4988482.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8116..8192)
                     /locus_tag="ACHJ12_000648"
     tRNA            complement(8116..8192)
                     /locus_tag="ACHJ12_000648"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8156..8158),aa:Val,seq:gac)
     gene            complement(8197..8273)
                     /locus_tag="ACHJ12_000649"
     tRNA            complement(8197..8273)
                     /locus_tag="ACHJ12_000649"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8237..8239),aa:Val,seq:gac)
     gene            8581..9837
                     /gene="ydhQ"
                     /locus_tag="ACHJ12_000650"
     CDS             8581..9837
                     /gene="ydhQ"
                     /locus_tag="ACHJ12_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4988483.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9878..11251)
                     /gene="mdtK"
                     /locus_tag="ACHJ12_000651"
     CDS             complement(9878..11251)
                     /gene="mdtK"
                     /locus_tag="ACHJ12_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4988484.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVKPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11466..12107
                     /gene="ribE"
                     /locus_tag="ACHJ12_000652"
     CDS             11466..12107
                     /gene="ribE"
                     /locus_tag="ACHJ12_000652"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4988485.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12147..13295)
                     /gene="cfa"
                     /locus_tag="ACHJ12_000653"
     CDS             complement(12147..13295)
                     /gene="cfa"
                     /locus_tag="ACHJ12_000653"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4988486.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDTDNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13586..14797)
                     /gene="punC"
                     /locus_tag="ACHJ12_000654"
     CDS             complement(13586..14797)
                     /gene="punC"
                     /locus_tag="ACHJ12_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4988487.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14910..15842
                     /gene="punR"
                     /locus_tag="ACHJ12_000655"
     CDS             14910..15842
                     /gene="punR"
                     /locus_tag="ACHJ12_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4988488.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15839..16864)
                     /gene="purR"
                     /locus_tag="ACHJ12_000656"
     CDS             complement(15839..16864)
                     /gene="purR"
                     /locus_tag="ACHJ12_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4988489.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17163..17252
                     /gene="cydH"
                     /locus_tag="ACHJ12_000657"
                     /gene_synonym="ynhF"
     CDS             17163..17252
                     /gene="cydH"
                     /locus_tag="ACHJ12_000657"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4988490.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17418..18587
                     /gene="ydhP"
                     /locus_tag="ACHJ12_000658"
     CDS             17418..18587
                     /gene="ydhP"
                     /locus_tag="ACHJ12_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988491.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18733..19314)
                     /gene="sodB"
                     /locus_tag="ACHJ12_000659"
     CDS             complement(18733..19314)
                     /gene="sodB"
                     /locus_tag="ACHJ12_000659"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4988492.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19442..20257)
                     /gene="mepH"
                     /locus_tag="ACHJ12_000660"
     CDS             complement(19442..20257)
                     /gene="mepH"
                     /locus_tag="ACHJ12_000660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4988493.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20591..20938
                     /gene="grxD"
                     /locus_tag="ACHJ12_000661"
     CDS             20591..20938
                     /gene="grxD"
                     /locus_tag="ACHJ12_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4988494.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20989..25605)
                     /gene="lhr"
                     /locus_tag="ACHJ12_000662"
     CDS             complement(20989..25605)
                     /gene="lhr"
                     /locus_tag="ACHJ12_000662"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4988495.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGKDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25698..26345)
                     /gene="rnt"
                     /locus_tag="ACHJ12_000663"
     CDS             complement(25698..26345)
                     /gene="rnt"
                     /locus_tag="ACHJ12_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4988496.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26448..26855)
                     /gene="gloA"
                     /locus_tag="ACHJ12_000664"
     CDS             complement(26448..26855)
                     /gene="gloA"
                     /locus_tag="ACHJ12_000664"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4988497.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26936..28033)
                     /gene="nemA"
                     /locus_tag="ACHJ12_000665"
     CDS             complement(26936..28033)
                     /gene="nemA"
                     /locus_tag="ACHJ12_000665"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4988498.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28070..28669)
                     /gene="nemR"
                     /locus_tag="ACHJ12_000666"
     CDS             complement(28070..28669)
                     /gene="nemR"
                     /locus_tag="ACHJ12_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4988499.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28772..29011
                     /gene="ydhL"
                     /locus_tag="ACHJ12_000667"
     CDS             28772..29011
                     /gene="ydhL"
                     /locus_tag="ACHJ12_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4988500.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29060..29956
                     /gene="ydhF"
                     /locus_tag="ACHJ12_000668"
     CDS             29060..29956
                     /gene="ydhF"
                     /locus_tag="ACHJ12_000668"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO4988501.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30037..30558
                     /gene="sodC"
                     /locus_tag="ACHJ12_000669"
     CDS             30037..30558
                     /gene="sodC"
                     /locus_tag="ACHJ12_000669"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4988502.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30559..32571)
                     /gene="ydhK"
                     /locus_tag="ACHJ12_000670"
     CDS             complement(30559..32571)
                     /gene="ydhK"
                     /locus_tag="ACHJ12_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4988503.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32571..33428)
                     /gene="ydhJ"
                     /locus_tag="ACHJ12_000671"
     CDS             complement(32571..33428)
                     /gene="ydhJ"
                     /locus_tag="ACHJ12_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4988504.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33431..33667)
                     /gene="ydhI"
                     /locus_tag="ACHJ12_000672"
     CDS             complement(33431..33667)
                     /gene="ydhI"
                     /locus_tag="ACHJ12_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4988505.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33868..34302
                     /gene="slyA"
                     /locus_tag="ACHJ12_000673"
     CDS             33868..34302
                     /gene="slyA"
                     /locus_tag="ACHJ12_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4988506.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34349..34816)
                     /gene="slyB"
                     /locus_tag="ACHJ12_000674"
     CDS             complement(34349..34816)
                     /gene="slyB"
                     /locus_tag="ACHJ12_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4988507.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35090..36199
                     /gene="anmK"
                     /locus_tag="ACHJ12_000675"
     CDS             35090..36199
                     /gene="anmK"
                     /locus_tag="ACHJ12_000675"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4988508.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36297..36626
                     /gene="mliC"
                     /locus_tag="ACHJ12_000676"
     CDS             36297..36626
                     /gene="mliC"
                     /locus_tag="ACHJ12_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4988509.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36685..37341
                     /gene="pdxH"
                     /locus_tag="ACHJ12_000677"
     CDS             36685..37341
                     /gene="pdxH"
                     /locus_tag="ACHJ12_000677"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4988510.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37470..38744
                     /gene="tyrS"
                     /locus_tag="ACHJ12_000678"
     CDS             37470..38744
                     /gene="tyrS"
                     /locus_tag="ACHJ12_000678"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4988511.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38803..39666
                     /gene="pdxY"
                     /locus_tag="ACHJ12_000679"
     CDS             38803..39666
                     /gene="pdxY"
                     /locus_tag="ACHJ12_000679"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO4988512.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39710..40315)
                     /gene="gstA"
                     /locus_tag="ACHJ12_000680"
     CDS             complement(39710..40315)
                     /gene="gstA"
                     /locus_tag="ACHJ12_000680"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4988513.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40421..41923)
                     /gene="dtpA"
                     /locus_tag="ACHJ12_000681"
     CDS             complement(40421..41923)
                     /gene="dtpA"
                     /locus_tag="ACHJ12_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4988514.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42534..43169)
                     /gene="nth"
                     /locus_tag="ACHJ12_000682"
     CDS             complement(42534..43169)
                     /gene="nth"
                     /locus_tag="ACHJ12_000682"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4988515.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43169..43864)
                     /gene="rsxE"
                     /locus_tag="ACHJ12_000683"
     CDS             complement(43169..43864)
                     /gene="rsxE"
                     /locus_tag="ACHJ12_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4988516.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(43868..44488)
                     /gene="rsxG"
                     /locus_tag="ACHJ12_000684"
     CDS             complement(43868..44488)
                     /gene="rsxG"
                     /locus_tag="ACHJ12_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4988517.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44492..45550)
                     /gene="rsxD"
                     /locus_tag="ACHJ12_000685"
     CDS             complement(44492..45550)
                     /gene="rsxD"
                     /locus_tag="ACHJ12_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4988518.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45551..47485)
                     /gene="rsxC"
                     /locus_tag="ACHJ12_000686"
     CDS             complement(45551..47485)
                     /gene="rsxC"
                     /locus_tag="ACHJ12_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4988519.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            complement(47478..48056)
                     /gene="rsxB"
                     /locus_tag="ACHJ12_000687"
     CDS             complement(47478..48056)
                     /gene="rsxB"
                     /locus_tag="ACHJ12_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4988520.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48056..48637)
                     /gene="rsxA"
                     /locus_tag="ACHJ12_000688"
     CDS             complement(48056..48637)
                     /gene="rsxA"
                     /locus_tag="ACHJ12_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4988521.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48714..49154)
                     /gene="ydgK"
                     /locus_tag="ACHJ12_000689"
     CDS             complement(48714..49154)
                     /gene="ydgK"
                     /locus_tag="ACHJ12_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4988522.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49240..49455)
                     /gene="ydgT"
                     /locus_tag="ACHJ12_000690"
     CDS             complement(49240..49455)
                     /gene="ydgT"
                     /locus_tag="ACHJ12_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4988523.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49728..49853)
                     /gene="blr"
                     /locus_tag="ACHJ12_000691"
     CDS             complement(49728..49853)
                     /gene="blr"
                     /locus_tag="ACHJ12_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4988524.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50096..51136
                     /gene="ydgJ"
                     /locus_tag="ACHJ12_000692"
     CDS             50096..51136
                     /gene="ydgJ"
                     /locus_tag="ACHJ12_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4988525.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51171..52172)
                     /gene="add"
                     /locus_tag="ACHJ12_000693"
     CDS             complement(51171..52172)
                     /gene="add"
                     /locus_tag="ACHJ12_000693"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4988526.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52276..53448)
                     /gene="malY"
                     /locus_tag="ACHJ12_000694"
     CDS             complement(52276..53448)
                     /gene="malY"
                     /locus_tag="ACHJ12_000694"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4988527.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53458..55050)
                     /gene="malX"
                     /locus_tag="ACHJ12_000695"
     CDS             complement(53458..55050)
                     /gene="malX"
                     /locus_tag="ACHJ12_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4988528.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            55225..56253
                     /gene="malI"
                     /locus_tag="ACHJ12_000696"
     CDS             55225..56253
                     /gene="malI"
                     /locus_tag="ACHJ12_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4988529.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56365..57132
                     /gene="hdhA"
                     /locus_tag="ACHJ12_000697"
     CDS             56365..57132
                     /gene="hdhA"
                     /locus_tag="ACHJ12_000697"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4988530.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57361..57951
                     /gene="uidR"
                     /locus_tag="ACHJ12_000698"
     CDS             57361..57951
                     /gene="uidR"
                     /locus_tag="ACHJ12_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4988531.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58339..60150
                     /gene="uidA"
                     /locus_tag="ACHJ12_000699"
     CDS             58339..60150
                     /gene="uidA"
                     /locus_tag="ACHJ12_000699"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO4988532.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60147..61520
                     /gene="uidB"
                     /locus_tag="ACHJ12_000700"
     CDS             60147..61520
                     /gene="uidB"
                     /locus_tag="ACHJ12_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4988533.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61559..62824
                     /gene="uidC"
                     /locus_tag="ACHJ12_000701"
     CDS             61559..62824
                     /gene="uidC"
                     /locus_tag="ACHJ12_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO4988534.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTNSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGGSVQKDVSNQIQSILEKGIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNDTA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(62869..64377)
                     /gene="ydgA"
                     /locus_tag="ACHJ12_000702"
     CDS             complement(62869..64377)
                     /gene="ydgA"
                     /locus_tag="ACHJ12_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4988535.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPALY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64478..65653)
                     /gene="manA"
                     /locus_tag="ACHJ12_000703"
     CDS             complement(64478..65653)
                     /gene="manA"
                     /locus_tag="ACHJ12_000703"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4988536.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            65852..67498
                     /gene="fumB"
                     /locus_tag="ACHJ12_000704"
     CDS             65852..67498
                     /gene="fumB"
                     /locus_tag="ACHJ12_000704"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4988537.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67641..69044
                     /gene="fumC"
                     /locus_tag="ACHJ12_000705"
     CDS             67641..69044
                     /gene="fumC"
                     /locus_tag="ACHJ12_000705"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4988538.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69041..69970)
                     /gene="tus"
                     /locus_tag="ACHJ12_000706"
     CDS             complement(69041..69970)
                     /gene="tus"
                     /locus_tag="ACHJ12_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4988539.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70046..71347)
                     /gene="rstB"
                     /locus_tag="ACHJ12_000707"
     CDS             complement(70046..71347)
                     /gene="rstB"
                     /locus_tag="ACHJ12_000707"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4988540.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNKATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71351..72070)
                     /gene="rstA"
                     /locus_tag="ACHJ12_000708"
     CDS             complement(71351..72070)
                     /gene="rstA"
                     /locus_tag="ACHJ12_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4988541.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72199..72534
                     /gene="ydgC"
                     /locus_tag="ACHJ12_000709"
     CDS             72199..72534
                     /gene="ydgC"
                     /locus_tag="ACHJ12_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4988542.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72531..73253)
                     /gene="folM"
                     /locus_tag="ACHJ12_000710"
     CDS             complement(72531..73253)
                     /gene="folM"
                     /locus_tag="ACHJ12_000710"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4988543.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73290..74672)
                     /gene="ydgI"
                     /locus_tag="ACHJ12_000711"
     CDS             complement(73290..74672)
                     /gene="ydgI"
                     /locus_tag="ACHJ12_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4988544.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(74858..75802)
                     /gene="ydgH"
                     /locus_tag="ACHJ12_000712"
     CDS             complement(74858..75802)
                     /gene="ydgH"
                     /locus_tag="ACHJ12_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4988545.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76326..77858
                     /gene="pntA"
                     /locus_tag="ACHJ12_000713"
     CDS             76326..77858
                     /gene="pntA"
                     /locus_tag="ACHJ12_000713"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4988546.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            77869..79257
                     /gene="pntB"
                     /locus_tag="ACHJ12_000714"
     CDS             77869..79257
                     /gene="pntB"
                     /locus_tag="ACHJ12_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4988547.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79282..80316)
                     /gene="tqsA"
                     /locus_tag="ACHJ12_000715"
     CDS             complement(79282..80316)
                     /gene="tqsA"
                     /locus_tag="ACHJ12_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO4988548.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            80728..81093
                     /gene="mdtJ"
                     /locus_tag="ACHJ12_000716"
     CDS             80728..81093
                     /gene="mdtJ"
                     /locus_tag="ACHJ12_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4988549.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81080..81409
                     /gene="mdtI"
                     /locus_tag="ACHJ12_000717"
     CDS             81080..81409
                     /gene="mdtI"
                     /locus_tag="ACHJ12_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4988550.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81448..82269)
                     /gene="ydgD"
                     /locus_tag="ACHJ12_000718"
     CDS             complement(81448..82269)
                     /gene="ydgD"
                     /locus_tag="ACHJ12_000718"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4988551.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82369..82452)
                     /locus_tag="ACHJ12_000719"
     CDS             complement(82369..82452)
                     /locus_tag="ACHJ12_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988552.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82545..82853)
                     /gene="asr"
                     /locus_tag="ACHJ12_000720"
     CDS             complement(82545..82853)
                     /gene="asr"
                     /locus_tag="ACHJ12_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4988553.1"
                     /translation="MKKVLALVVAAAMGLSSTAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            83124..83228
                     /locus_tag="ACHJ12_000721"
     CDS             83124..83228
                     /locus_tag="ACHJ12_000721"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4988554.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(83277..84530)
                     /gene="ynfM"
                     /locus_tag="ACHJ12_000722"
     CDS             complement(83277..84530)
                     /gene="ynfM"
                     /locus_tag="ACHJ12_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988555.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFNSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT        21386 a        20834 c        21972 g        20433 t
ORIGIN      
        1 gctttgtaat tttaattgat aacgatcaat gttaagggct cagaataatc acattatttt
       61 ttctcctaac cataaaggat tagtttatcg gcgttaaatc ttttctcata cactatcggg
      121 ttagggaaaa tatctggtgt tggggacaga gtcagcagcg tattagttga atgcttaata
      181 tcaggctgct gaattgtcag cactgtgttt gcgaagagga tgcgaaataa atgaacccgg
      241 ttgatcgtcc actattagat attggcttaa cgcgattaga attcttgcgt atatcaggaa
      301 aaggccttgc tggtctaaca attgcccctg cgttactgtc acttttgggt tgcaaacagg
      361 aagatattga tagcggcaca gtagggttga taaatacacc caaaggggtg ctggtgaccc
      421 agcgagcgcg atgcacgggt tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg
      481 gctcagtagg gacattcttc tcccgtatca aaatccatcg caattatttc tttggcgaca
      541 acggggttgg ctctggcggc ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc
      601 gtcaatgcaa agagccgcaa tgcatgaacg tctgcccgat tggtgcgatt acctggcagc
      661 agaaagaagg ctgtattacc gtcgatcata aacgctgtat tggctgtagc gcctgtacta
      721 cggcatgccc gtggatgatg gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg
      781 tgttatgcgg tgaatgcgca aacgcctgcc caacaggggc gttaaaaatt atcgagtgga
      841 aagatattac tgtgtgaatc ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa
      901 tatattaaga gtcaatctca cgacaggaaa tattaccctc gaagattcca gtaagtttaa
      961 aagttttgtc ggtggcatgg gcttcggcta caaaattatg tatgacgaag taccgctagg
     1021 cacgaaacct ttcgatgaag cgaataaatt agtctttgct accggcccat taactggatc
     1081 tggtgccccc tgtagttctc gcgtaaatat cacctcactt tctactttta ccaaaggaaa
     1141 tttagttgtc gatgcccata tgggtggctt ttttgcagcg caaatgaaat tcgctggata
     1201 cgacgtcatt attatcgaag ggaaggcgaa atcaccggta tggctgaaga ttaaagatga
     1261 caaagttagc ctggaaaaag ccgatttctt atggggaaaa gggacgcgcg caacgacgga
     1321 agaaatttgt cgattgacca gtccggaaac ctgtgtggcg gctattggtc aggctgggga
     1381 aaaccttgtt cctctctctg gcatgttgaa tagccgtaac cacagcggtg gtgcgggaac
     1441 tggcgcaata atgggttcga aaaacctgaa agcgattgcg gttgaaggga cgaaaggggt
     1501 caacattgcc gatcgtcagg agatgaagcg tctcaacgat tacatgatga ctgaacttat
     1561 cggtgcgaat aacaaccatg tcgtgccaag tacgccgcaa tcgtgggcag agtattcgga
     1621 tcccaagtca cgttggacag cacgtaaagg gctgttttgg ggcgcggctg aaggcggtcc
     1681 aattgaaacg ggggaaattc cgccaggcaa tcagaatacg gtcggctttc gtacctataa
     1741 atccgttttt gacttaggtc cggcggcaga gaaatacaca gtaaaaatga gcggctgcca
     1801 ctcttgcccg atccgttgta tgacccaaat gaatattcct cgggtcaaag agtttggcgt
     1861 gcccagcaca ggtggtaaca cttgtgtagc aaactttgtc cataccacca tcttcccgaa
     1921 cgggccgaaa gattttgaag ataaagacga tggtcgtgtg attggtaacc tggttggtct
     1981 gaatttgttc gatgactacg gcctatggtg taactacggg cagctgcatc gcgactttac
     2041 ttattgttac agcaaaggtg tgttcaagcg tgttctgcca gctgaagagt atgcggaaat
     2101 tcgctgggat caactggaag cgggtgacgt taacttcatt aaagattttt actaccgtct
     2161 ggcgcatcgt gtgggtgagc tgagtcacct ggctgatggt tcatatgcca tcgcagaacg
     2221 ctggaatttg ggtgaagagt actggggcta cgcgaaaaat aaactctggt cgccgtttgg
     2281 ctatccggtt caccatgcca atgaagcgtc agcgcaggtc ggttccattg ttaactgtat
     2341 gttcaaccgt gactgcatga cacataccca tatcaacttt attggttccg gcttgccatt
     2401 gaaactgcaa cgtgaagtgg cgaaagagct ttttggttct gaagatgcct acgatgaaac
     2461 caaaaactac acgccaatca atgacgcaaa aattaaatat gctaagtggt cgctgttgcg
     2521 ggtctgtttg cataacgccg tcaccctgtg taactgggtc tggccaatga ccgtttcgcc
     2581 gctgaaaagc cgtaattacc ggggcgatct ggcgcttgaa gccaaattct tcaaagcgat
     2641 caccggcgaa gagatgactc aggaaaaatt agatttagct gcagagcgta tctttacgtt
     2701 gcatcgtgcc tacacggtaa aactgatgca aaccaaagat atgcgtaatg aacacgatct
     2761 tatctgttcc tgggtattcg acaaggatcc gcagatcccg gtctttactg aaggtactga
     2821 caaaatggat cgtgacgata tgcatgcttc gctcacgatg ttctacaaag aaatgggctg
     2881 ggacccacag cttggttgtc cgacccgcga aacattgcag cgtctggggc tggaagatat
     2941 tgccgccgat ctggcagcac acaatctact gcctgtgtaa ggaatgggtg agatgaacca
     3001 tcgggatgaa ttgccgttag cgaaagtcag cgaagttgac gaggcgaagc ggcaatggtt
     3061 acaggggatg cgtcaccccg ttgatacggt aaccgaacca gaaccggctg aaatcctggc
     3121 agagttcatt cgccagcatt ctgcggctgg gcagctggtg gcgcgcaccg tgtttctctc
     3181 gccaccctat tcggttgcag aagaggaact gtcagttttg ctggaaagca ttaagcagaa
     3241 cggagactac gcggatatcg cttgtatgac gggaagtcag gatgattact attactcaac
     3301 gcaggccatg agtgaaaact acgcggcgat gtctttgcaa gttgtggagc aggacatttg
     3361 tcgcgccatt gcacacgccg ttcgttttga gtgccagacg tatcctcgtc cttacaaagt
     3421 cgccatgctg atgcaagcgc catattactt ccaggaagcg cagattgaag ccgcgatcgc
     3481 cgcgatggat gttgcacctg aatatgccga tatacggcag gtggagtcat cgacggcggt
     3541 gttgtacttg tttagcgagc gatttatgac ctatggcaag gcttatggct tatgcgaatg
     3601 gttcgaggta gagcagtttc agaacccctg aaatttcagg gagatgtcat ggatagggtg
     3661 gacagttgca gccactgcat taggggataa tcagatgtcc ttcactcgac gcaaatttgt
     3721 tctggggatg ggaacagtaa tcttttttac tggttctgcg tcttctctgt tagcgaacac
     3781 gaggcaagaa aaggaagttc ggtatgccat gattcatgac gagtcacggt gcaatggctg
     3841 taatatttgc gcccgcgcat gccgtaaaac taaccacgtc cccgctcagg gaagccgttt
     3901 atcgatagca cacattccag tcaccgataa cgacaacgaa acgcagtatc acttttttcg
     3961 ccagtcatgt cagcactgcg aagatgctcc gtgcatcgac gtatgcccga caggggcgtc
     4021 gtggcgtgat gagcagggga tcgtgcgggt agaaaaatcg cagtgcatcg gctgcagtta
     4081 ttgcattggc gcttgtccgt accaggttcg ctaccttaac cctgtcacta aagtggcaga
     4141 taaatgtgat ttctgcgctg agtcccgact ggcaaaaggc tttccgccca tttgcgtcag
     4201 cgcctgtccg gaacacgcat tgatctttgg tcgcgaagat agcccagaaa ttcaggcgtg
     4261 gttgcaggat aataaatact atcaatatca gctacctggt gccgggaaac cgcatctgta
     4321 tcgccggttc ggtcaacatt tgattaaaaa ggaaaatgta tgaacccgtc gcaatatgct
     4381 gaacagtttc agagccagtt agcgaactat gtgccacagt tcactcccga attttggccg
     4441 gtgtggttga tcattgccgg agtattgctg gttgggatgt ggttggtgct ggggctgcac
     4501 gccttgcttc gtgctcgtgg cgtgaagaaa tcagtcaccg attatggtga gaagatttat
     4561 ctttactgca aagcggtcag attatggcac tggtcgaatg cgttactctt tgtattgttg
     4621 ctggcaagtg ggctgataaa tcactttgcg ctggtgggcg caactgcggt taaaagtctg
     4681 gttgcggtgc atgaagtttg cggatttttg ttactcgcat gctggctcgg ctttgtgctg
     4741 atcaatgccg ttgggggtaa tggtcaccac tatcgcattc gtcgtcaggg gtggctggag
     4801 cgagcggcaa aacaaacgcg attttatttg tttggcatta tgcaggggga agaacatcct
     4861 ttcccggcaa caacccagtc taaatttaat cccttacagc aggtcgccta tgttggtgtt
     4921 atgtatggat tgctgccgtt gttactattg acggggctgc tgtgtctcta tccgcaagcc
     4981 gtgggagatg tgtttcctgg cgtaagatac tggttattgc aggcgcattt tgctctggca
     5041 tttataagcc tcttttttat cttcggtcat ctttatcttt gcaccacggg gcgtacgcca
     5101 cacgaaacct ttaaaagcat ggtcgatggc tatcaccggc actaacgaat gatcattaca
     5161 cgagcagacc tgcgagaatg gcgtatcgga gcggtgatgt atcgctggtt cctgcgccat
     5221 ttcccacgcg gcggtagtta tgccgatatt catcatgcac tgattgagga aggatatacg
     5281 gactgggcgg agagtctggt tgagtatgcg tggaaaaaat ggctggcgga tgaaaacttc
     5341 gcgcatcagg aagtttcctc tatgcaaaaa ctggccacag accccggaga gataccattt
     5401 tgcagtcagt ttgcccgcag cgacgatcat gctcgcatcg gctgttgtga ggataacgcc
     5461 cgaatcgcaa ccgctgggta tgccgcgcaa attgccagta tgggttatag cgtgcggatt
     5521 ggaagtgtcg gctttaacag tcacattggc agctccggtg aacgcgcacg ggttgctgta
     5581 accggtaatt cttcgcggat aagcagcgct ggagatagta gtcggattgc gaataccgga
     5641 atgcgagtac gcgtttgtac attgggtgaa cggtgtcatg tcgccagtaa tggtgacctg
     5701 gtgcaaatcg ccagctttgg cgcaaacgcc agaattgcca acagcggcga caatgtacac
     5761 atcattgcca gcggtgaaaa ttcaacggta gtgagcacgg gcgtggtcga ctcaattatt
     5821 ctcgggccag ggggaagcgc cgcactggca tatcatgacg gcgaaagagt tcgcttcgcc
     5881 gtcgccattg aaggtgaaaa caatattcga accggagtcc gctatcgtct taatgaacag
     5941 catcagtttg ttgagtgctg atagctttac ctttaatcaa acgaaagacg ccgacgagca
     6001 cgggatgcag gctttacgac cgcccgagcc atcgcctcac caatttctgc acatgccgta
     6061 agtccctgaa cgaaaggctg gtcgtgcatc aaccagggca acgcgccgaa cgctacgcgt
     6121 ccacgaatat cttcgggttg ctgtaacgta taatcttcgc caacatcagg gatttcatcc
     6181 cctgttttct gtaattgctc gcgtagccca gggaaaggaa tatctttcac tttaagcgga
     6241 cgctgtccgc gggcatcaat aaaaacgtca aacgagtagc tgttgtcttc cgttttcagg
     6301 acggtgcgcg actcattaat ttccattttg tagtcttcac cgagggcgag aatatgaatg
     6361 attcccgctt cacgtaaggc aagtaggcga cgaatagact ctgaagggat tgccgcataa
     6421 ttatcgatga aaaccctggc aaggccttta ctgaaccgtt tatggtcgtg ttcattcaga
     6481 tgaggaacaa tttcctgtac ggcttcatgc aggcgcagaa tgacataacg ccagggaaca
     6541 gtatgttttt ctcgtttatt gcgttcgact tcctggagat ttttttctgc ccagtcaaat
     6601 gggtcgcgtt gtttgcgctc ggcaaaccag gcttgagcaa aggaatcgac attcaggctc
     6661 tctaaggcta tgcgttggct ccagtctgga tcagcaaact tgatttcctc tactatcaat
     6721 ctaaataccc gatccaaaag gccctcttca cctttttgaa tctcagcatt taatgcctga
     6781 tcggtgacga tgtgtaaggg ctcgtaggga atagggcaat agaaatcggc ttcgggtaaa
     6841 atacccgtgc gcgacatcaa cgtaatattt agcttttcac ttgcgttatc gcggttaaaa
     6901 acgacgtgtt gtttatcatc ttcaatgaac gaaccatgct gaatagccac tgccattgcc
     6961 gcatccagtc cgctcaagga tgttcccata atacccacgt tacacgcatc gacctttgct
     7021 tccatcaggc ctgaccacgg actgggaaaa tacgttctgg ttgcttcttc ttcatcaggc
     7081 cagacgtgac ccgtggcaat caccgctaaa tcaaacgtct cgctgggtaa atcctgattt
     7141 gtagcgagca tgacgccagc atttgtaatt tgcagatcgg taacctggca tgattcataa
     7201 acagccactg caaatttttg ctgtcgtgcc tggtctacta atcgtaaaaa ttgatcgcgg
     7261 aaatattcgc ccagcagaat tcgcggtaaa aactgacgat cgtgcaaggt ttctttttta
     7321 acgccataac gctggagatg gctggcttct tgcttttgta gccattcgag atacgtacaa
     7381 ttaatcggcg gtatttcaat actggcaata tttgccagca tcatttttga gttttcctca
     7441 tcactgtatg gcatcccgac accggcctcg tcagcctgct cgaagataga aatagaaagt
     7501 ggagtttgtt gctgtagaag cgagaataag gtgtagatcc ccgtaggccc ggcacccaca
     7561 atagcaattt ttttcattgt tacttacctg tacgcattct ttcgaattta gtgttatcag
     7621 ataagtttgg ttgagatttt tagaatttga gaaatttcag acagggatga aaagggcgct
     7681 aaccgccctt gattaaattc tgtcaggcga gttttgcctg attgatttga ctcagtggct
     7741 cattgacatc gaaaactttg gcgacaactt cctcaacgcc gagatttttc agtcgggcag
     7801 tgtgtttttc cagataggca agggcgcttt tttcatcagt gaaaaggtag atcccaccgg
     7861 cttcgtggtt cttttcactt tcggtccata ccttccacag aaaaccaggt tcctgattaa
     7921 tcgactcagc aagcggttta agctgctcag ccattgcgtc gccaaatggg ccattgaaag
     7981 caaaatgaag ttgtaacaaa gttgccatag cgattctcct tctgataacg catacgaaga
     8041 cagtgtaaat cttttaacgt attggataca gagaagaaac agagggaaaa aggagaagac
     8101 cagattaaga aaatctggtg cgtccgagtg gactcgaacc accgaccccc accatgtcaa
     8161 ggtggtgctc taaccaactg agctacggac gcaggatggt gcgttcaatt ggactcgaac
     8221 caacgacccc caccatgtca aggtggtgct ctaaccaact gagctatgaa cgcaacgttg
     8281 taggtgacaa cggggacgaa tattagcggc agagtgggaa ggtggcaaga ggcaaaacgt
     8341 aattttctgc gctatttcga ccgtttgcag agcttttaag caaattggct atattttgtt
     8401 gatttgcaag ggcgattttt attcaggatc gcatttacat ctgatacaac ccaataattc
     8461 gtgtatcaga gtttaaacag agttgtttgc caccatctca aatctgtcgt ggataaatcc
     8521 tccgtagcct ttacacttag gtagcaataa aggcacacag atgcagcaaa ggaataaatc
     8581 atgggatctg atgcgaaaaa cttgatgagc gacgggaatg tgcaaattgt taagaccggc
     8641 gaggtcattg gcgcgacgca acttactgaa ggcgagttga ttgttgaagc tggcggaaga
     8701 gccgaaaata ccgtggtcac gggggctggc tggttgaaag tggcaaccgg tgggatcgcc
     8761 aaatgcacac agtacggtaa caatggcacg ctatcggtca gcgacggtgc cattgccaca
     8821 gatattgttc agtccgaggg aggcgcaatt agtctctcta cgctcgctac ggttaatggc
     8881 cgccatcccg aaggtgaatt cagcgttgat cagggttatg cctgcggttt gttgctggaa
     8941 aatggcggta acctgcgtgt actggaagga catcgcgcgg aaaaaatcat tctcgatcaa
     9001 gagggcggcc tgttggttaa tgggacaacc tcagcggtcg tggtagatga aggtggtgaa
     9061 ttgttggtgt atccaggtgg ggaagccagc aattgtgaga ttaatcaggg cggcgttttt
     9121 atgctggccg ggaaagccag tgatacgttg cttgctggtg gcaccatgaa taatctcggt
     9181 ggtgaagact ctgacactat tgttgagaat ggatccattt atcgtctggg gacggatggc
     9241 cttcagctct acagttccgg taagacgcaa aacctgtccg tgaatgtggg tggtcgggct
     9301 gaagtgcatg ccggtacgct ggaaaatgcg gtaatacaag gtggaacagt gatccttttg
     9361 tcacccacca gcgcggacga aaattttgtc gtagaggaag atcgcgcacc ggttgaactg
     9421 accgggagtg ttgcattact ggacggcgct tcaatgatta ttggctatgg cgcagatctg
     9481 caacaatcaa cgattactgt acagcagggc ggtgtgttga ttctcgacgg cagtacggta
     9541 aaaggtgaca gtgtcacttt tagtgttggt aacatcaatc tgaacggcgg aaaactgtgg
     9601 ctgatcactg gtgcggcaac gcatgtgcaa ctgaaagtga aacgcctgcg cggagaggga
     9661 gcgatttgcc tgcaaaccag tgcgaaagaa atctcacctg acttcatcaa tgtgaaaggg
     9721 gaagttaccg gggatataca cgttgagata acagatgcca gtcggcaaac tctgtgcaac
     9781 gctctgaaat tacagccaga cgaagacggg attggcgcaa cgctccagcc tgcgtaaaaa
     9841 cacaggcgca acccgtgaag gttgcgcctt gtctttatta gcgggatgct cgttgcagaa
     9901 tgataactga cggcagacgt tgcaggaacc gcatacgcag catcatcata atggctgccg
     9961 acgtcaggcc aataataaag cctatccaga agcctgctgg ccccataggt ttaacgacca
    10021 gatcggtcag tgccagaata tagccgcttg gcaagcccag cacccagtaa gccgtaaagg
    10081 tgatatagaa aatggaacgc gtatctttat aaccacgcaa aatcccactg ccaatcacct
    10141 ggattgagtc agaaatctga tataccgccg ccagcaacat caaatgcgca gccagcgtta
    10201 caacctcggg attgtcgttg tacaacaggg cgatttgctc ccgcagtgaa accgtgaata
    10261 tggccgtcag ggttgccata cagacaccca ccataagccc ggtccgcgca gcggtttgcg
    10321 catccagcgt tgagccctga cccagacgat aacctacgcg gatagttacc gctgccgcca
    10381 gcgacattgg aagcacaaac atcagtgaac taaagttcag ggcaatctgg tgtcctgcga
    10441 catcaacaat accgagcgga gacactaaca gagccacgac ggcaaacagt gtcacttcaa
    10501 agaatagtgc cagcgcaatc ggcaaaccga gttgaatcag tcgtttcata accgcaggat
    10561 cgggtttcgc ggtgcctttt tcgttacgaa tatcgcgcat ggagcgggcg cgtttaatgt
    10621 aagaaaccat ggcaaggaac atgacccaat acaccgccgc agtagccacg ccacaaccaa
    10681 cgccaccgag ctcaggcata ccgaaatgac cataaataaa gatatagttc accggaatgt
    10741 tcaccagcag gccgataaag cccattacca tacccggctt ggtttttgcc agaccttcac
    10801 attggttacg ggcaacctgg aagaacagat agcccggcgc gccccacaac aacgcacgca
    10861 gataacccac ggctttgtcc gccagagccg gatcgatgtt ttccatggag cggataatgt
    10921 aacctgcatt ccacagcaca agcataatga gaacggaaac aaaacctgcc agccagaaac
    10981 cttgtcgcac ctgatgcgca atgcgctcac gtcgaccgga accatttaat tgcgcgataa
    11041 ccggcgttaa tgccagcagc agtccgtgac caaagaggat cgccggaagc cagatagaag
    11101 taccgatagc gaccgccgcc atgtcggtgg cactatagcc gcccgccatc acggtatcga
    11161 caaaacccat cgcagtttgg gcgatttgcg cgagaatcac cgggattgct aatgctaata
    11221 acagacgcgc ttcactgata tacttctgca cgtgaacacc ttttatttgt agttatatga
    11281 gagactaaaa agccgcctga atgggcagca aaaagaagaa gcaggggaaa ttcagtctat
    11341 tgtagcgagg atttattaat tctccagtga aaaaatagtc attcttaccc cttcactggt
    11401 aagggcgttt gccacctgct attgtgctga acagaatgtc ttaactgatt tcaggagttg
    11461 taagtatgtt tacggggatt gtacagggca ccgcaaaact ggtgtcgatt gacgagaaac
    11521 caaattttcg tacgcatgtg gtggagttac ccgaccacat gctggacggc ctggaaacag
    11581 gtgcttccgt ggcgcataac ggttgctgcc tgaccgtgac ggaaattaac ggcaaccatg
    11641 tcagttttga cctgatgaaa gaaacgttac gcattaccaa tcttggcgat ttaaaagtgg
    11701 gggattgggt aaacgttgag cgtgcggcga aattcagtga tgaaattggc ggacacttaa
    11761 tgtcaggtca tattatgacc accgctgaag tggcgaaaat attaacctcg gaaaataatc
    11821 gccagatctg gtttaaagtc caggatagtc agttgatgaa atatatcctg tacaaaggat
    11881 ttattggcat cgacggtatt agcctgaccg tcggcgaagt cacgccaacg cgtttttgcg
    11941 tccatttaat tccggaaact ctggaacgca cgactcttgg gaagaaaaaa ctcggcgcac
    12001 gcgtcaacat tgaaattgat ccacaaactc aggcagtggt agatacggta gaacgtgtgc
    12061 tggcggcacg agaaaatgcc atgaatcaac caggtacaga agcctgatct gaaatcgccc
    12121 ccggagaggg ggcgatagaa tagcctttag cgagcgactc gaaggccgtt ttcaacaccg
    12181 cgtgagaaca cgacctgcca gagctgaata tcacgggcgc ggaaagcacc tgcacaggca
    12241 ttcagataat aggtaaacat tcgtttaaag cgttcactgt agttatccgc aatttctggc
    12301 catgcggcga ggaatcgttc ataccacgcc atcaacgtag tatcgtaatc agcaccgaag
    12361 ttatgccagt cttccatcac gaagtggggt tcgctggatt gagcaatctg gcgtacagag
    12421 ggcaggcaac cgttcggaaa aatatattta ttaatccagg gatcaacatt cagatcggtt
    12481 tttttcgaac cgatagtatg gagcaggaat ataccttccg gtttcaaatt acgatccacc
    12541 accgcaaaat aggtatcgta atttttcggt ccgacgtgct cgaacatccc cacagaaaca
    12601 atacgatcaa actggtcgtt caggtcacga taatcttgca gcaaaatggt gacatccagg
    12661 ccttcacagc gttcctgagc cattttttgc tgttcggcag aaatggtgac gccgaccacg
    12721 cttacgtcat agttagatgc catgtagtgc gccagtccgc cccagccgca gccaatatcc
    12781 agtacgcgca tccctggttt taactgcaat ttttcacaaa tcattttgag cttcgcctgc
    12841 tgggcagatt ccagattatc ggtatctttc cagtaagcgc aggaatattg catgaaggga
    12901 tcaagcatgc ggctgaacaa gtcattaccc aaatcgtaat gctccttgcc gactatccag
    12961 gcacgttttt tactctgcag attgaagaga cgagcgctgg caatacgcag cgtgtctttg
    13021 aaatgatggg ggagttggtt ctcgagacct gcgcgtaaga ctttgctaaa aaacatatcc
    13081 agtcggtcac attcccacca gccatccata taactttcgc ctaaccccaa agagccttct
    13141 tgcagaacgc gtttaaaaaa atcggggttt ttcacacgaa tatccgccgg ggcagaaccg
    13201 ttaatggcta taccggcacg gctaagtaat tcgttggcga tacggtacca gttgtcatcc
    13261 ggtacactga cttcttctat acacgatgaa ctcatagttt ctccatcact ggtcggtgat
    13321 cagaacctta aaacagcgta gacgcttttt gggctttgtg agaaatctcg cagaaaaaac
    13381 cgcgagtcta taatccgacg tagaacagag ggaaaggagg aacctttgcc gaaatgacca
    13441 tccatagtga gtcgggaacg atcctgtccc cgtaaaaaca taatatttat cgtagcttta
    13501 acaactcgaa attagtatag gccgctaatt tcaatcatac aacaaaattt tgttagcagc
    13561 ctaagtataa gtatatcgat atagatcagt gtgattcgct atgagcgact tcggcattgc
    13621 catgattctg gcagccaact tcttcacaac gttgcatcat gtaacccaga gcgaccagca
    13681 ctactgttga taacatcacg ctggtggtgg tgagcaatgg cgtgctgata ctgatcagcc
    13741 aggaaactac cagacttgcg aggaagcaca gacccagttg tagagtgttc tgcaacgctg
    13801 cggcgcgacc agttgcgtgt gggaagggac gcagcgcctg ggcgacaaca atcgggtaga
    13861 tcgcgccatt ggcaatcgcc atcacacaga atgggatcag gatttcgacc agcgacacat
    13921 ggctaatgaa gcctgcagcc caggtcgcaa tgacgctgac tgcaaacagc accagcaacc
    13981 acggtaataa ctgcttgcct tgccatttct gcagcgcggc gcgacagcca taaccaccaa
    14041 tcagaaacgc gatagtttgc gggacataac ttaaaccaat aactgccggg ctgtagccca
    14101 tttcactaag gatgaacggt gaaccggtca gccatgcaaa aaaactggct gaacaggccg
    14161 cgtatatcag cacgttgccg cgataggttt tagaacgtag caggtcggta aaggtcagac
    14221 catcctgact attgttacgg gccttcgtcg tgggtttgag ccagaaaata ggcagaatca
    14281 gcaccacggt aatggcaaac agggtggcga aaatcgcctg ccaggaaaaa tggaccagca
    14341 gccagcttcc taacagagga gccagggccg gagatagacc caccagcggc atgatggtcg
    14401 caaaaattcg gttaactttc tgtgaaggat aataatccgt cactaacgct tgccagataa
    14461 ccgccgcggc gcagacaccc acagcctgta caaaacgcaa taccagcagc gtagcggcgt
    14521 tttctaccca cagcatcccc agactaccta acgcaaaaat tgtcaggccg attaataaca
    14581 ccggtttacg accataacgg tcggagagtg gcccccacag aagctgggct gcggcaaaac
    14641 cggcaaggaa cagactaagg ctggcactga cagcagacgc aggcgtttgc aggtcggcct
    14701 gtatggcggc gaaagcaggc agatacatat cggttgccag aaaaccgagt acgctcaaac
    14761 ccgccagcca gactaaaaat cttttcccag gttgcatgta tattctctaa atctctcaat
    14821 taaacaccgc cgcaaagtgt agggagtgaa attagtgttg tgaaacggta atatttgatg
    14881 actggtttca aaaattttgc aggcagacta tgtggtcaga atattcactc gaagttgttg
    14941 atgcggtagc gcgtaatggt agttttagcg ctgcggcaca ggagctgcat cgcgttcctt
    15001 ctgcggtcag ctataccgtg cgtcagctgg aagagtggct ggcggtgccg ctctttgaac
    15061 gacggcaccg tgatgtggag ctgaccgctg ctggagcgtg gtttctcaaa gaagggcgct
    15121 ctgttgtcaa aaaaatgcag atcacccgcc agcaatgtca gcagatagcg aacggctggc
    15181 gcggtcagtt agctatcgca gtggataata ttgtcaggcc agaacgtaca cggcagatga
    15241 tcgttgattt ttatcgccat tttgatgatg tcgaacttct tgtctttcag gaagtgttca
    15301 acggtgtctg ggatgcgctt tccgacgggc gcgtggaact ggcgattggc gcaacacgcg
    15361 cgattccggt aggcggtcgt tatgccttcc gggatatggg gatgctaagc tggagttgcg
    15421 ttgttgccag ccaccacccg ctggcgttga tggatggccc attcagcgat gatacgttgc
    15481 gcaactggcc gtcgttggtg cgcgaagaca cctcgcgaac gctacctaaa cgtattacct
    15541 ggttgctgga taaccaaaaa agagttgtcg tgcctgactg ggaatcatcg gcaacctgta
    15601 tctcggcagg attatgcata gggatggtgc caacacattt cgccaaaccg tggcttaatg
    15661 aggggaagtg ggtagcactt gaattggaaa accccttccc ggattcggca tgttgtctga
    15721 catggcagca aaatgatatg tcgcctgcgt taacctggct gcttgagtat ctcggcgata
    15781 gtgagacgct gaataaggag tggctgcggg agccggaaga gactcccgca acgggtgatt
    15841 aacgacgata gtcgcggaac gggccgtcag ccacggagcg gcgttcaatc aagcgcggat
    15901 gcacttcaat ggactgcggt tcttcacgtt tgttgacgat acgatccaac agcatgttga
    15961 acgctgtttc acccagcgaa tcttttggct ggtggatcgt ggtcagcgcc ggcgtaaaat
    16021 agcgggcgtt gcgcacgtta tcataaccga tcagcgaaac atcctgcggg acgcgcagac
    16081 ccatttcatc agcagcacaa agtgcgccca ttgccatgat atcgccacca cagaagacgg
    16141 cagtagggcg atgcggctgc gacagtattt gctgcatggc gcgataacca gattcgggtt
    16201 caaagtcacc ctgaacaatc cagctttccg gcaccttgat catcgcttct tccatcgcct
    16261 tcataaaacc ggcaaggcgg cctgcgccgg tgttacgttc cagcgggccg gggatgacgc
    16321 cgatttcgcg gtgaccgcgt tcaatcagat aacgcccggc catgtagccg ccttcgaacg
    16381 cgttatcaat gaccgcatcg gtgaagtcag cttttgcttc accccagtcc atcaccacca
    16441 ttgggatatg gcgatactct tccagcatcg ccagcaacgg ctctgggtac tcagaacaca
    16501 tcaccagcag accatcgacg cgtttttgcg ccatcatcga cagataagcc cgctgtttct
    16561 caagattgtt ccacgcatta cccagaatca gggtgtaacc tttctggaag caattttttt
    16621 caaccgcttc aatgatctcg gcaaaatagg ccgcttcgct gctggtcgcc agcaaaccga
    16681 tagacttggt gtggttaacc ttcaggctac gcgccaccgc gctaggggag tagtgtaatt
    16741 ctttaatcgc tgcccacacg gcgttgcgcg tttcttcagc gacgaaacgt gttttgttga
    16801 ttacgtgtga cacagttgta gtggaaacgt ttgctcgttt cgctacatct tttattgttg
    16861 ccattccatt tcactccaga ccctaacttc agctcctgca aaatcgcaag gtaaacgttt
    16921 gcctttacac accttacgca caacaaaagt gttgcggtac gccggaaaaa cgacacggag
    16981 cgtcaggaag gggtcaatgg ccagtacgca aatagtgtaa gcgtggaatt ttggccgatc
    17041 ttgacgaaaa ggggaagtgg taaaaacact tatcattata aatgacggga gattttttca
    17101 tcacagtgtg taaaaatgcg taatatcact ttttgtgtgg taataaaaaa ggagaaaact
    17161 tgatgagcac cgaccttaaa ttttcactgg taacaacgat tatcgtcctc ggtttgatcg
    17221 tagccgtggg tttgactgcc gcgctgcact gatttcctct cgagggagcg cgttctccct
    17281 cgcatctcct tcctgtttgt tagcaaaatg gcaaaagtgt tttctctttt tgttattttc
    17341 atttttttgt gattgctgtc atgctttcgc cactttaatg aatatcgcgt aatgacgcga
    17401 agaggtggag ataaggcatg aaaattaact atccgttgct ggcgctggcg attggcgcgt
    17461 ttggtatcgg gacaacggag ttctcgccaa tgggtttgtt gcccgtcatt gcgcgcggtg
    17521 tggatgtctc gattcccgct gccggaatgt taatcagtgc ctatgcagtt ggcgtaatgg
    17581 ttggcgcgcc gctgatgacg cttctacttt ctcatcgtgc ccgccgcagt gcgttgattt
    17641 tcctgatggc aattttcacg ctcggcaacg tactttccgc catcgcgccg gattatatga
    17701 ccctgatgct ttcacgcatt ttgaccagcc tgaatcacgg agcatttttt ggtttgggtt
    17761 cagtcgtggc cgcaagcgtg gtgccaaaac ataaacaggc cagcgcagtt gccactatgt
    17821 ttatggggtt aaccctggca aatatcggtg gcgtgccggc ggcgacctgg ttgggtgaaa
    17881 ccatcggctg gcggatgtca tttctggcaa cggcggggct gggagtgatt tcaatggtaa
    17941 gtctgttctt ctcattacct aaaggtggtg caggggcacg acctgaagtg aaaaaagagc
    18001 tggcggtatt aatgcgtccg caggtgctgt ctgcattgct gacgacggta ctgggagctg
    18061 gtgcaatgtt tactctctac acctatatct ctccggtact acaaagtatt acccacgcaa
    18121 caccagtgtt cgtcacggca atgctggtgc tgattggtgt cggattctct atcggtaact
    18181 atctcggcgg caaactggca gatcgttcag ttaacggcac gttgaaaggc tttttgttgt
    18241 tgttgatggt gattatgctg gcaatcccgt tcctggcccg caatgagttc ggcgcagcta
    18301 ttagcatggt ggtgtggggc gcagcaacct ttgcggtcgt accgccgtta cagatgcgcg
    18361 tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc agtcaatatt ggtgccttta
    18421 atcttggaaa tgcgctggga gcagctgctg gtggtgcggt aatttccgct gggctgggat
    18481 acagctttgt gccggtgatg ggagcgattg tcgcgggact ggcattattg ctggtgttta
    18541 tgtcagccag aaaacaacct gaaacagttt gcgttgctaa cagctaatta aacaaaaacg
    18601 cagaaggata accttctgcg ttacagttgc tgacaacgtg cgatttatct tgccgaatac
    18661 gctgttaacg cctcatccgc gttggcatgg taagcgaagc gcatcaggca atgctgcatt
    18721 tgccatcagt tattatgcag cgagattttt cgctacgaat tcccagttca ccagcgccca
    18781 gaagtgctcc agatagccag gacgtgcatt gcgatagtcg atgtaataag cgtgttccca
    18841 gacatcaacg gtcagcagcg gagtcgcatc ggtggtcagc ggagtacccg cgttagaggt
    18901 tgaaacgata gccagtttgc catcgctgtt tttcaccagc caggtccagc cagaaccaaa
    18961 gtttttgatc gctgcatcag taaactgcgc tttgaaatcg gcaaagctgc caaaagatgc
    19021 ggcgatagct tcagcgactt ttccagtcgg ttcgccaccg gcgttcggtg ccaggcagtt
    19081 ccagtagaaa gtatggttcc agacctgagc tgcgttgttg aatacgccac cttcagagct
    19141 gcgaataatc tcttccagtg atttaccttc aaacgcggta cctttaatca ggttgttcag
    19201 gttagtgaca taagtctgat gatgcttgcc gtagtgatac tcgatagttt ccgcagaaat
    19261 gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt aattcgaatg acattgctac
    19321 tctcctttat tattaatttg catacgtaca atagccttat tgtgcgtatg gatagggtag
    19381 caaattaaaa ggtgagacaa aagatgaact taccctgttg ccgtaacaac agggtaaagt
    19441 tttagcgaag tgtttttggg gtcattaccc gacgagcgcc aacatagtgg cgctgccagt
    19501 agtcttcact gagagaagtg atttgaattt cctgacctgt gcgcggtgac tgaataaatt
    19561 tgccgttgcc gacatacacg ccgacatgat cggctgtgcc gcgtccctga gtacggaaaa
    19621 agaccaggtc gccgtttttc agttcactac gttcgattgg cgctgcatca cgcaggtgat
    19681 acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa atctttataa gcgtaataaa
    19741 ccaggccgct gcaatcaaaa ccggtacgcg gtgagctgcc accccaacga tatggcttac
    19801 caatttgctg catcagttta ttcattgcca ctttcgtagc tttttgcacc ttcgctttgt
    19861 gcgcgtcggc aagagttcct gacgcggcat ttttgacttt cacacaatgc gatttatagc
    19921 ccttacgttt ggtacacttt tcggtaaagc tggctgaggc cgttttattg acggcgtgtt
    19981 tgctgcgagt tttggcgttt ttagaagcgg taatggaaga ttttttaacg gaagaagcgg
    20041 ttttgctttt ggtcgtactt ttactggcgg cttttttcgc tgttttctgg gtctttttgg
    20101 tggttgccgt gcttttcttt ttatctgctt ttttggtgat atgagtggta gcagaactct
    20161 ccctggcttg ctttgaagca tgggccatag gcgttaaagg cagggtggta aaaagtaaag
    20221 cacagagcgt gatcgaaata cggtttatcc gcgccactga gcgtccctct gttaaggcag
    20281 gtctatcatg cctgcgtctg atttacaaaa cttgatgatt ctaatgcata gaagaagaga
    20341 aagttaatag cttttattgt taaaaaatac gtttcgttgg cgagcgcaaa aaaatgcagc
    20401 agtgaaagct aaaaatggtt ataaatacgc cagtttgccg gcatcagaga agaaacgaat
    20461 atttgaaatg caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta
    20521 ttttccgatg ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg
    20581 aagcaagaca atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat
    20641 cctgctgtac atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt
    20701 ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga
    20761 cattcgtgcg gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga
    20821 cggcgagctg gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca
    20881 gcagctgatc aaagaaactg ccgctaaata caagtctgaa gagcccgacg cggaataatc
    20941 cgtcctgcaa tactgacgga tgcgaacggg catccgtcag tcctttctct atccccaatc
    21001 cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg
    21061 ccggaccggt agatcattca cttctgttag cgtaaagcgc agccgtggtt cgcgacgcag
    21121 tgcggtagtc agcgcgtgga aaacctctgg ggcgagtaat tcctcttttt cctgccagac
    21181 cagcattttt ttgccacctt gcgccagata gagtaacaat ttgccgccag aaacgaccac
    21241 cagcgcaccc gcccgacgcg ttggaaccag cgttgccgga tgtgcaggcc agggcagaag
    21301 atttccccac acattagccg gatcgttggc agagagcgcc actggtgtat agtggcgcgt
    21361 ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg
    21421 cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt cttccatact
    21481 tcgacaaagc gtttgcatcg atggaaaccc gccagggata ttttcggcta tcaccgcctg
    21541 acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca tcctttcggt
    21601 atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg gtgtgttgta
    21661 agatacccgc ggcgagacgg gttgcgcata gacaggacgt ccacgacgag cccggtgact
    21721 gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta acggtgccca
    21781 aatgtcgctg gtgatgacgc cttgccagac cagcgcccat aacgcctctt gcagggcaga
    21841 gagatcaacc gattcgccga ttttgtcgcg tatccgttgg ctgatttgtt gtgcaaacca
    21901 ggctcctccg tcagccagaa cagcgactat cgcttgttgc agcgccgaac gattcgcctg
    21961 atccgcttcg gcgggagtga acgattctgc agcatattcc tgtagatgca gtgccaccag
    22021 gccgtcatct tcacccagtt ttttttgccc cgaccagata accgcaccgg ttgccagtaa
    22081 ttcatcgagc atttctgacg aatagtcgcg tacgcgggca ggcaggatct ggctttccca
    22141 gagtgaggcg ggtaaaccga ctccggcaag ctgttcgatc acccgcatca cgccatctac
    22201 gccctcataa acgcctggcg atgttgaggc aaagagcgcc gggctaccat cggtggcggg
    22261 taatacgccc tgacgttcca gcagcaatcg cgcataggtc gtggctgcaa cgggacgcgt
    22321 cgcttctctg gcggcttgca gcgagcgcaa acgcagacga cgaaatactt catcgctcac
    22381 ccagatgtct tgttgcagat tcatcaccag accctgttca cgcagttgct gaagctgctc
    22441 ttcgacaatg gcaataccga gactaaactc atgagccagt tgttcagccg tgaccagagc
    22501 atgagcccgg agatagcgca gaaagagatc gcgaagcggg taactgactc tatgtaaata
    22561 aatctctggc aatgactctg gtagtcgtac gccgagggca tcacgcagcc tggcggcatc
    22621 atccatacag gcaagacgct cctgtccgct aatcatcgct gggaagattc gttttactgc
    22681 aagaagattt tccagatacg acgcaacctc ttcactgctg cctgtatgcc gttgcgccag
    22741 gtcttcaacg gtcattggcc ccagttcgcg cagcaggtcg aacaatcctt cttcaccttt
    22801 cgctcttctg ccaggagcca gtcgttgcaa ctcttcttcc acctggcgaa tgacctgcgg
    22861 gtcgagtaat tcccccggat cgacctgtcc gagtagattg cgcagtaact cgctgtccag
    22921 cgacagtacg gatgcccggc gctctgccag cggggcgtcg ctctggtaca taaattccgc
    22981 gacatagccg aacaataaac ttgtggcgaa aggcgagggc gtagtggtcg ttacatcgga
    23041 tatttgaatt tcgccaccgt tcaggcgacg catcaaacgt tccagtgcgg gaagatcata
    23101 aacatcttgc agacattcgc gtagggtttc gagaatgacc ggaaaatccg gatatccctg
    23161 agcgatttcc agcaactgac tggcgcgcag ccgttgttgc caaagcgggg tgcgatggcc
    23221 cggagtgcgc cccggcatta ataatgcccg cgcggcgcat tcgcgaaaac gggcggcgaa
    23281 aagtgccgag ctgcctaccg cctcgcggac aatttgcagc aacttttctg gttcaaacaa
    23341 aaaaatcgcg gcgtcgggca atttaccatc agtgtcagga atgcgcgcaa caatgccgtc
    23401 atcactggcg accacagacg cgtcagcgcc ccatagggca tgtattcgcc cggtaatcgc
    23461 cagcgcccag ggttcatgca cccgtcttcc atagggagag tgcaaaataa tacgccagtc
    23521 gccaatttca tcacggcacc gttcgaggac cagatggcga ctccccggaa cgatgcccgt
    23581 cgcgttgcgt tgctcgtcaa tcaacccctg aatattggcg ttcgtatttt cttctgccag
    23641 ccacggggga atagtcccgg aaaagaacgc gccatccgcc agcaaatgaa gaaaatcgcc
    23701 gatcatctcg cctaattcag ccggacgtcc gttaccttca ccacgccaga aggggagccg
    23761 ggcagaacga cccggagcag gagtcacaat cacctgatcg cgggtgattt gctggatccg
    23821 ccatgaggta gcgccgagag tgataatgtc gttcacccgc gactcatata ccatctcctc
    23881 atccagttca cccacccgcc gcgaaccggc cttttcttca ccttcgggta ataacacgct
    23941 atacatgcca cgatccggaa tggtgccgcc gctggtaacc gccaacaatt gagcgccagg
    24001 tcgggcggtc aatatcccgg tctccctgtt ccagaccagt ttggggcgaa aagcagaaaa
    24061 atcgccagag ggatagcgcc cggaaagcat atccagcgtg gcgtcaaaaa cacgtcttgg
    24121 cagatctttc cacggtgcgg cacggcgtac gcgggagtac cattcgtcta cctgtaatgc
    24181 ctccatcgcc gcggcggcaa cggtttgctg cgcaaggacg tcgagtggat tatgcggcgg
    24241 tgtcaggttt tccagcctgc ctgcgaacat acactctaca atgactgcgg aatcgactaa
    24301 gtcacgccgg gtacgaggga aaaacagccc tttagataca ccgccaacct gatgtccggc
    24361 gcgaccaatg cgttgtaacc cactggcaac agaaagcggc gttgccacct gaatcaccag
    24421 atccactgcg cccatatcaa tccccagttc gagactggag gttgcgacca cgcagcgtaa
    24481 ctcacccgat ttcagcgcct gctcggtgat tgctcgttgt tctttagaga cggagccgtg
    24541 gtgtgagcgg gcaataaaaa cgtcgctact ttgtacacgg ttagaggttg cgccggaggt
    24601 cgactcgaaa tgggccgtat caacggcgat agacggggaa cgctgtaagc gtgcggcgta
    24661 aagctcattt aatcgtgccg tcagtttttc cgccagccca cgtgaattag taaagacaat
    24721 ggtcgagcga tggcgcaaca cttcatcaag gatacccgtt tcaatatatg gccagatgga
    24781 gccttcccgg ccggcatggc tgtctttgcc ggtgccgctg gcgaccgatg agacatcatc
    24841 catattggcg accggtacga caattcgtat ctgcggatgg cgcattgcgg gcgggttgac
    24901 taccgtaacc gggcgatcgc caccaagaaa tgctgccaca tcgctggctg agcgcacagt
    24961 ggcagaaagg ccaattcgct gtgctgaggt gtggagcagc gcatcgagcc gctccagact
    25021 taacgccaga tgcgcaccac gtttactgcc cgctaccgcg tggacttcat caataattac
    25081 cgtttcgacg ccgcgtagcg tttcgcgcgc gcgggaggtc agcatcagat agagtgattc
    25141 gggtgtggtg atcagaatat ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg
    25201 cgtatcgcca gtacggatcc ctacgcgaag attgacttcc gtttcgccgc gccgccgccg
    25261 ttcatcggca atacccttca acgggagctg caagttgcgc tgaacgtcgg tgcccagagc
    25321 ttttatcggt gaaatataga ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc
    25381 ttcgccgcct tcgcggaaga gccgatcgag ggcgtagaga aatgctgcca gcgttttccc
    25441 ggagccggtc ggtgcaatca ccagcgcatg ttcgcttcgc gccgccacat gccaggtttg
    25501 cgactggaca gcggtcggct gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga
    25561 aaacacgtcc ggcaggagcg atgaagggtc tggattatct gccattaacg ctattccccg
    25621 tgaatatcat gcttgatgcg tggaatagcc tcactataga tgcctgaatc attgcatgtc
    25681 atcaggcatc gactcgatta cacctcttcg gcggcaggta gcggccagcc tcccagacgt
    25741 ttccagcggt tgacgatttc acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg
    25801 gagtgcgcct gggtgctgtc gaagtccatg ccagcggtct ggcaagcctt tgacaatacg
    25861 gtttgtccga gtgccagccc ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac
    25921 gggttacgtt tcagtgaggc gcgttctgcg gcggccatca taaagctgtg atcaaaattg
    25981 gcattgtgcg ccaccataat ggcgcggtta cagccgctcg ctttaatacc tttacgtaca
    26041 actttaaaaa tttcgtgcag cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc
    26101 gggtcaatgc cgttgaaggc gagggcctct ggctgcaaat ttgcgccgac aaatggttcg
    26161 acgtggaaat gtaatgtggt gtccggcatc agccagcctt gttcatccat tttcagggtg
    26221 atggcggcaa tctcaagcag cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg
    26281 atcacaacag gataaaaacc acgaaaacgg tcgcacagac cggtaagttg agcgttatcg
    26341 gacatctgat tctcttaata cggataaatt gcagcgcgca ttatgacaaa tattacttgt
    26401 cgatgcagta aagatgcggg cgcgatgagt tcacgcccgg caggagatta gttgcccaga
    26461 ccgcgaccgg cgtctttctc ttcgattaac tcaattttgt aaccgtccgg atcttccaca
    26521 aacgcgataa ccgtagtacc gccttttacc ggacccgctt cacgggtcac gttacccccg
    26581 ttttgacgga ttttttcgca cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca
    26641 taagcagtgc cgagttcgta tttatccacg ccccagttgt aggtcagttc aatcaccgct
    26701 tcttcggttt ccgggccgta gccaacaaac gccagtgagt atttgtattc cgggttttcg
    26761 ctggtacgca gcagtttcat gcccagcact ttggtataaa aatcgatgga gcgttgcaga
    26821 tcgccaacgc gcagcatggt atgaagaaga cgcatctttt tatcctcaaa atggctaaca
    26881 gattcaaaat gttgttttag tatagcggcg gctttacgcc gctcgcaatg ttggattaca
    26941 acgtcgggta atcggtatag ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg
    27001 ggttaagctc agctttgcgc tgcaagcggg cgaccagatc cgggttcgca atccagtcac
    27061 gaccaaatgc caccgcatca attaaccctt tgccgatcag cgtttcagct ttttctactg
    27121 tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg
    27181 catcagtata cggttcaccc cccgcccaat ctggttctga catatgcaga taagcaatgc
    27241 cgcgtttacc cagttgttca atcagataca gtgcatcggc ttcttcattc gcgccgttat
    27301 ccgtgttctg gaaagtaccg attggcgaaa cgcgaatgcc aatgcgatcg gcaccccatt
    27361 cttcaatccc ggcatcgacc acttccagta ccagacgtgc gcgattttcc acgctgccgc
    27421 cgtactgatc ggtacgatgg tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt
    27481 gagcagagtg gagctctacc agatcaaaac cggcttcacg cgcgttagca atggcctgac
    27541 ggaaatcatt gacgatacct ggaatctctt ccagttcaag cgcacgcggc atggatgttt
    27601 caacacggat cgcctgacca ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg
    27661 ctgaaggcgc taccggtgcc tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg
    27721 tgtgccacag ctgcacggcc atatgaccat tttcagcatg aacgccagcg gtgatttttt
    27781 tccatgcggc aatttgctcc ggactatgga tgccaggcgc acctgcatat ccttttgcct
    27841 gggcagaaat ttgcgtggct tcactaataa tcaaaccggc actggcacgt tggcgatagt
    27901 attccgccat caacggggta ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg
    27961 gtgccataaa aatacggttt gccgccgtga tcgcgcccac tttcagtggg gaatacagtt
    28021 tttcagatga cataaaggct cctggtcgtc tggtgataaa aaatgcctgc taaacggcag
    28081 gcgtcgcaat aatgtttttt acatgggcca gcgcgttttc cagtggctcg aaactgcgcg
    28141 aaattttggc ctgcagattc gcgccaagcc acagtgcgta aagcacttgt gcctgttgta
    28201 gcggttcgcc acaaaaggtt aaacaatggt tctcacggcc attttccagc gcctgagaga
    28261 gcagggcgat cacgccgcga gcacctttat ccatcgcgct gcgcatatct tctgacagat
    28321 cgcacacttc ggcagagagt tttactgtca ggcaaccact gatggttcca tgttggcaaa
    28381 actggttcag tgtttgctgg taataagcca gtatgcggtc gcggtagtta ccttcgccgg
    28441 attgcagcaa ctcagtcagt cgctggtgat atgcggcgta atgacgctca agcatggcaa
    28501 cgccaaacgc ttctttagag cgaaagtagt gatagaagga ccctttcggc acttcagcgg
    28561 tttttagtaa ttcgcttagc cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc
    28621 ccgtcgccag gagatgttcg cgagtatcat gttcggtgtg tttgttcatg cgttggagtg
    28681 tagtagacca gtcggtctag tgcaagcaaa gttgctactt tggacattaa cagcttcaat
    28741 aatggtaata atcttatctg aggagggcga agtggcggag caattagagt tctttcccgt
    28801 ccagagcccg tgccggggaa tttgccagtc tgacgaacgc gggttttgtc gcggctgttt
    28861 tcgtagtcgt gatgagcgtt ttaactggaa taaaatgagc gatggcgaaa aacaagaggt
    28921 gctgcgcctt tgtcggcagc gattaatgcg caaattacgc gcaaataagc cagcgtcatc
    28981 agacgaaccg gagcaaccat cactcttttg aacctaatct tgcgtatact cctctcatat
    29041 ctgataagag gaagcgatta tggttcagcg tattactatt gcgccgcaag gcccggagtt
    29101 ttcccgtttt gtgatgggct actggcgatt gatggactgg aatatgtccg cccgccagct
    29161 ggtcagtttt attgaagagc atctggatct cggcgtgacc accgtggacc atgctgatat
    29221 ttatggtggc tatcagtgcg aagcggcgtt tggcgaggca ctgaaactgg cacctcacct
    29281 gcgtgaacgg atggaaatcg tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa
    29341 cgtcattggt cattacatca ctgaccgcga tcacatcatt aagagcaccg aacagtcgct
    29401 aattaatctc gcgaccgatc atctggatct gctgttaatc caccgaccag acccgttaat
    29461 ggatgccgat gaagtggcgg acgcgttcaa acatctgcat cagagcggca aagtgcgtca
    29521 ttttggcgta tcgaacttta cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt
    29581 tacccttgcc actaatcagg tggaaatatc cccggtgcat cagccgttac tgctggatgg
    29641 cacgctcgac caactacaac aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg
    29701 tggtcgtctg tttaatgatg attatttcca gccgctgcgt gatgaactgg ctgtggtggc
    29761 agaggagtta aacgcgggct cgattgaaca ggtggtttac gcctgggtat tacgtttacc
    29821 atcgcagccg ctgccaatta tcggttcagg taaaattgag cgcgtacggg cagctgtcga
    29881 agcagaaaca ctgaaaatga cccgtcaaca atggtttcgt atccgtaaag cggcactggg
    29941 gtacgacgta ccgtaagcac ttttaggaat agccgccgtt caaaaatgtg tcactggttt
    30001 acacttattc aggaatgcac aatgaacgga ggtcctatga aacgttttag tctggctatt
    30061 ctggcgctgg ttgttgcaac cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc
    30121 gtcacgtcgc aaggggtagg gcagtcaatt ggtagcgtca ccattactga aaccgataaa
    30181 ggtctggagt tttcgcccga tctgaaagca ttaccccccg gtgaacatgg cttccatatt
    30241 catgccaaag gaagctgcca gccagccacc aaagatggca aagccagcgc cgcggaatcc
    30301 gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg aagggccaga aggtgccggg
    30361 catttaggcg atctgcctgc actggtcgtc aataatgacg gcaaagctac cgatgccgtc
    30421 atcgcgcctc gtctgaaatc actggatgaa atcaaagaca aagcgctgat ggtccacgtt
    30481 ggcggcgata atatgtccga tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc
    30541 tgtggtgtaa ttaagtaatt aagaggccag cgtaccttgc ggtggtgctt gctcaagttg
    30601 cgaaagcgag cagtacagcc gccagacaat tgccgccagc tcacgggcgg caggttgatg
    30661 atgacgggca aggctgtcgc aaatccgctg taattcttca agtgtggccg ccagtgattt
    30721 ttgctgaacg ccacgctcac tcatcactcc ccgcaacaat gaaatacagt tatcccgtac
    30781 tcgcgataac ggatcggaac gcgattccca gtcgcgcaat tgccagacaa catgagaaca
    30841 gttcagcagc actacacccc agcgtaataa ccaacggcga gccagcgcat cctggctgtt
    30901 actcaactga ctgacgtgat gataagtgag cgattcaaat tcgctttcac tcagtgttgg
    30961 atggcggctt agctgatcga caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt
    31021 acgagcatcc gatcctggac gcagaatggc gaacgctagc caagccagcg caacgccaac
    31081 gatttttgcc agattatcgt taagaaaatc agcaaaatca tacaccgggg gattagtgac
    31141 agcgataaac gaacccataa aaacaatcag ttgcccccac agtgcggcaa atttgggcat
    31201 ctgcaattta agaagctgca ttgtcgccag cagtggaaag agaaacagta aaaattgcca
    31261 cagatcgcta atctggacca tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa
    31321 caccagcgtg cgcatcagca gtgacaacga cttaaacggt gctgcgacgg cggagtagag
    31381 tacgcagcta attgctgcca gcgttaatgc attggcaccg gcatcccatt gcgatgcaat
    31441 actccatgcg ccaatcatca tcaacgtaca aaatgtacgc agcccgctcc acatagcttc
    31501 ggcgttgtcg gtatgacgag ccagagcgga tgtccgtgcg agtctggcat gatcatctac
    31561 accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc aaaaataacg
    31621 tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc gtagcggggc
    31681 gataatacgt gcgacggtgt aagcatctgt ttgcgaactg gcgagcgccg tcagcaattg
    31741 ttcgagaatt tctcgcgtgg cacctggcgg tgagggccag ttgagcaaca tacgtcgcag
    31801 gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa ggcgcgcgtt
    31861 ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat tcatggtcag
    31921 tatctgccca atcaccccct catgtgctgc acgaatggca tcgtttgttt caggctgcca
    31981 gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcgg ttaaaagggc
    32041 tgtagcatcg gaactgctag gcaggatcat catcatcatg ccgccgcaca aaataccgac
    32101 tatcacctcg caaacgcgcg cctgagcgat atcccacagc tggctggcct cagtaatatt
    32161 aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg catacgcgac
    32221 gttattcgtg aagtgcgcac aggcccaggt acaaaagcca agccacgccg acatgctcaa
    32281 tagaaaaaac cacggctcat tgagcgtatg cccggcaaga agcagtgccg caatggctcc
    32341 cagtaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa cggtgggaaa
    32401 gctaaccact gcagccgagg tcatcgccca atagggttca tccagattca aataataggc
    32461 aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc attgcgccag
    32521 cgtggccttg aaccagggca aattgcgcaa tgaccatgac gatgcgttca ttattgacct
    32581 acggcaatgc tacaggtggt gcccgacacc agcgtgacat cgcctggaac tttatccagc
    32641 gcaaagcgga cgggaacgcg ttgggcgaga cgaacccagg ggacgttagg tttcacatcc
    32701 ggaataaggc ttgagtcact ttcaacgctt tgatcataaa tcgcccgacc gatactggaa
    32761 acgtgacccc gtaacgtttt gttatcacta tagagggtaa tttgtgctgg tgcaccttcg
    32821 cggatatggc gcaacttggt ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg
    32881 acaagggcaa acagcggttt cccggtgtca gcgtaatcgc cgatgcgggt agtgaggtta
    32941 gtcacccaac cactcaccgg ggcgcgaatt tccgtttgcg ccagttgcca ttgtgtctgt
    33001 ttcagcgtgg cctgtgcggc attaacactg gcctgcatcg ctttaacatt gaggttagcg
    33061 gtatcgagtt cttcggcaga aataaaattt tgtgagagat gacgacgacg attcgcttcg
    33121 ttattggctt ttgcgaggtc agattgcgct ttcgccagct gagcctgtgc gttcagctcg
    33181 gcgatctgaa agggcgtttt atcgatcgtg agcagaagat cccccgcgtt aaccagttgg
    33241 ttgtctttta tattcagctc aacaatacgt cccgacacct gtggggtgat agaaaccttt
    33301 tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca tgtaataatt ccacagccac
    33361 catccggcaa gaaccgcaac tatcgctaca atgattgttg agaaatactt aattgtttta
    33421 attgacatag ttaccacgca atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca
    33481 gcgataaatc cattaacaag ggatgccaga tgtcaccggc gtagatccag ccacgaagca
    33541 ggcggtgtac gacaagccag atgacgaatc caaacgcgaa ggctttgaaa aacggaggaa
    33601 aatagacgga cgcaccgaac accagatctt gtaagggcaa tcctgttgca ttgagcataa
    33661 acttcacaaa gtagattcct ttacgaccga atatattgcg ttagattaat aaatattctt
    33721 taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag
    33781 caatacattt gttttagcaa tacaattgct gcacactatt ctaaaagccg cataatatct
    33841 tagcaagcta attataagga gatgaaattg gaatcgccac taggttctga tctggcacgg
    33901 ttggtgcgca tatggcgtgc tctgatagac catcgcctga aaccgctgga gttaacacaa
    33961 acccattggg ttacgttaca caatattcat cagttacctc cagaccagtc gcaaattcaa
    34021 ctggcaaaag cgattggcat cgagcagcca tcactggtcc gtactctgga ccaactggaa
    34081 gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa
    34141 ctgacggaaa aggcagagcc actgatcagc gaaatggaag ctgttattaa caaaacccgc
    34201 gcggaaatat tacatggcat ctccgcagag gaactggagc aactgattac gctcatcgca
    34261 aaacttgagc ataatatcat tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg
    34321 tcagtaacct gaccacacgc aaacttattt agcgcggaga aacggtcacc tgactgccat
    34381 tgctggccag tacgacacgt tggcccggag agaaacgagt gttgccttgt ttctgtacca
    34441 ccatgatggt attaccatcg tctttacgaa tttccagctc gacaccctgc gttttgttca
    34501 ttgcactctg tacgccctga ccagctacgc cacctgcaac agcgcctgct gcagtagcca
    34561 gagaacgccc ggttccgcca ccaacagtat tccccaggaa accaccaaga acagcaccgc
    34621 caattgcacc gataacgttg gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa
    34681 cgatggtgcc atagctgaca ttctgtactt gtttcgcttc agaagcggta taaacatccc
    34741 ctgacagggt gtcgttatta acacaaccga caagagacag acctaccatt gaaacaacca
    34801 atacgcgttt aatcattgaa aaatctcctg ttcaccatga aacgccacgc aagcatccct
    34861 catggtcaaa gtatatggca tatgtgaatc catggttaca tgttctacca aatatgagca
    34921 aatcataatg aaagtaggat caaccaatta taaacaaaac attcatgatg attattgatt
    34981 tgaagataat gttttactac caacggtaaa aatcggtcat tccgcatggt attcacgaaa
    35041 agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg
    35101 ccgctttatt ggcgttatgt caggcaccag ccttgatggt gttgatgttg tgttggcgac
    35161 aatagatgaa caccgggtcg cacagctggc aagcttgagc tggccgatcc cggtgtctct
    35221 gaaacaggct gtactggata tttgccaggg ccagcagctt acactttcgc agtttggaca
    35281 gcttgatact caactcgggc gactttttgc tgatgcggtc aatgccttgc ttaaggaaca
    35341 aaacctgcag gcgagagata tagttgcgat cggctgtcac ggtcaaaccg tctggcatga
    35401 accgacgggc gtggcaccac acactttaca gattggcgat aacaatcaaa ttgtggcacg
    35461 caccggaatt acggttgtcg gtgattttcg ccgtcgcgat attgccttgg gaggacaagg
    35521 cgcaccgctg gtacctgcgt tccatcatgc cttgctggct cactcaaccg agcgacgaat
    35581 ggtgctcaat attggcggca tcgccaatct gtcactgctc attcctgggc agccggttgg
    35641 cggctacgat accggtcctg gtaacatgct gatggatgcc tggatctggc gtcaggccgg
    35701 taaaccttac gataaagatg ccgagtgggc acgggcgggt aaagttattc tcccactgct
    35761 gcaaaatatg ctcagcgacc catatttctc gcaacctgca ccgaaaagca ccggacgcga
    35821 atactttaac tacggctggc tggagcgcca tttacgccat tttccgggtg ttgatccccg
    35881 tgatgttcag gccacactgg cagaactcac cgccgtgacc atttctgaac aagttttgtt
    35941 gagcggtggc tgcgaacgat tgatggtatg tggtggaggt agtcgtaatc cgctactcat
    36001 ggcgcgtctg gcggcattac tgccaggcac agaagtcacc accaccgatg ccgttggcat
    36061 tagtggcgat gacatggaag cattggcttt cgcctggctt gcctggcgga cgctggcggg
    36121 attaccagga aatctgcctt ccgtcactgg cgcaagccag gagacggtac tgggggctat
    36181 tttccctgct aacccgtgat atcagagtta actgaatttt tcttaccgcg tctgccgtta
    36241 cacttggaac attagggagg gcgtattgcc ctccagacca ggaaagtctt cgggaaatgt
    36301 ctatgaaaaa actgttactg atctgtttgc cggtgcttct caccggctgt agcgcgttca
    36361 atcaactggt tgagcacatg caaaccgcta cgctggaata ccagtgtgat gaaaaaccgt
    36421 tgacggtcaa actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac
    36481 tgcatctcaa acagggcatt tcagcgtctg gtgcacgtta cactgacgga atctatgttt
    36541 tctggtcgaa aggcgatgaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact
    36601 gtcagttaca aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc
    36661 cactgacaat ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat
    36721 ctgcgccgtg aatacaccaa aggcgggtta cgccgtcgcg atcttcccgc cgatccatta
    36781 accctttttg aacgttggct ctctcaggcg tgtgaagcca aactggcaga ccctaccgcg
    36841 atggtggtcg ctaccgtgga tgaacatgct cagccttatc aacgcatcgt tttactcaaa
    36901 cattacgacg aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa
    36961 attgaaaata atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg
    37021 atggtgatcg gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc
    37081 cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc
    37141 cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg
    37201 ccattgccga gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag
    37261 ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc gagaaaatga tgcgtggaag
    37321 attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg
    37381 attctggcac tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag
    37441 gtgcagtcgt tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc
    37501 aagagcgggg gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc
    37561 aaggcccgat cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc
    37621 atcttgttcc attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc
    37681 tggtaggcgg cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc
    37741 tgaacaccga agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt
    37801 tcctcgattt cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg
    37861 gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga
    37921 tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca
    37981 ctgagttttc ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg
    38041 atgtggtgct gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc
    38101 tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag
    38161 cagacggcac caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa
    38221 ccagcccgta caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct
    38281 tcctgaagtt cttcaccttt atgagcattg aagagatcaa cgccctggaa gaagaagata
    38341 aaaacagcgg taaagcaccg cgcgcccagt atgtactggc ggagcaggtg acccgtctgg
    38401 ttcacggtga agaaggtttg caggcggcaa aacgtattac cgaatgcctg ttcagcggtt
    38461 ccttgagtgc gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg
    38521 ttgagatgga aaagggcgca gacctgatgc aggcactggt cgattctgaa ctgcaacctt
    38581 cccgtggtca ggcacgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac
    38641 agtccgatcc tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac
    38701 tgcgtcgcgg taaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag
    38761 ggggagtgag aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc
    38821 gctatccagt ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttcctatg
    38881 cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa
    38941 tacggcaaat ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc
    39001 attgccgcca ttgataaatt acacacctgt gatgccgtgt taagtggcta tctgggatcg
    39061 gcggagcagg gtgaacatat cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag
    39121 gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg
    39181 ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat
    39241 ctggttgagc tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg
    39301 gcagcgcgcg aactcattgc gcaagggcca caaattgtgt tggttaaaca cctggcgcga
    39361 gctggctaca gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agcctggcat
    39421 atcagccgtc cgctggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg
    39481 agcggtttac tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc gctggaacat
    39541 gtgaccgctg cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg
    39601 caagtggtgg ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag
    39661 ctctgaaata tctcatttcg gcccacaacg acctgtgggc cgatggatat tactttaagc
    39721 cttccgctga cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgctg
    39781 caatgtgctc taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa
    39841 acagataggc atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg
    39901 cctcgttcac atattgcagc ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt
    39961 ccggtgtatc agggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat
    40021 tcagccattc gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt
    40081 cggggacgct gtcggcaaga tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt
    40141 catccagcag caatgcaggc acctgcccct tagggttaac gacaaagtaa tcgtcaccgt
    40201 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
    40261 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
    40321 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct
    40381 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
    40441 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
    40501 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
    40561 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
    40621 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
    40681 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
    40741 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
    40801 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
    40861 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
    40921 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
    40981 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
    41041 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
    41101 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
    41161 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
    41221 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
    41281 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
    41341 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
    41401 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
    41461 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
    41521 aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
    41581 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
    41641 cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
    41701 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
    41761 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
    41821 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
    41881 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
    41941 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
    42001 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
    42061 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
    42121 cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactgcactg agaggggggt
    42181 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
    42241 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
    42301 atgattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
    42361 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
    42421 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
    42481 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
    42541 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
    42601 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
    42661 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
    42721 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
    42781 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
    42841 gcagcacgat cttccggaac ctcgccatta tgctgctcca gcaagatacg gcaggttttg
    42901 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttcacccct
    42961 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc
    43021 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
    43081 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
    43141 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
    43201 cacgttcggc agttgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc
    43261 ctgccagcat cagtcccagg ccaataaatg cacctggagg cagcatcgcc agcaggaaag
    43321 gggagtcggt gtggaaaatt tccacgcgta atacttttgc ccagctacct aacagcgcat
    43381 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
    43441 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
    43501 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
    43561 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
    43621 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
    43681 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
    43741 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
    43801 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
    43861 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
    43921 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
    43981 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
    44041 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
    44101 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
    44161 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
    44221 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
    44281 ctggcgcagt taccagatag caactctgtg ccagcgcatt gttatagcgt tcggctggca
    44341 gcacctgatc aaataatgcc tcttgttgca gactggcctg ttcagcaatc gtcgttttgg
    44401 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
    44461 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
    44521 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
    44581 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc
    44641 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
    44701 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
    44761 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
    44821 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
    44881 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
    44941 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
    45001 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgtccgc
    45061 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
    45121 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
    45181 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
    45241 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
    45301 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
    45361 gtttgagtac gagagcttcg gctaacagag cactaaccga tgccaacagg atctgaacga
    45421 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
    45481 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
    45541 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
    45601 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
    45661 tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
    45721 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
    45781 gcgctttggc gcgggcgata gctgcttcaa cggcagtttt acgtggatca gcaacggttg
    45841 ctgcgtcgtt agtttgctgc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
    45901 cccgtgctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
    45961 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
    46021 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc gctttttcgc
    46081 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    46141 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aggggaatat
    46201 tgctcgggca aacccacgcg caagccccac attcaatgca atcagcaatg ttatgcgtgg
    46261 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatccg
    46321 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    46381 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    46441 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    46501 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    46561 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    46621 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    46681 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    46741 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    46801 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    46861 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    46921 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagcc gcgttgataa
    46981 tcaacgtttc aatcttatct ccgccaccct gcaatttaac gcctgtcggg aatcctgcac
    47041 cgcccagccc ggctacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    47101 tgcgataatc ggcccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    47161 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    47221 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    47281 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    47341 gtttcagtgg aataacaaaa cgctgcgcca gtggtacctg gcgcaggggt gtaccgttgg
    47401 actgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    47461 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    47521 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    47581 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    47641 atggcacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    47701 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    47761 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    47821 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    47881 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    47941 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    48001 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    48061 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    48121 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    48181 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    48241 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    48301 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    48361 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    48421 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    48481 acgccagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    48541 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    48601 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    48661 cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg ttattacgga
    48721 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    48781 gccaacaaag taaacagcaa ctggcgaacg gctatgccat cttccacggg tgaaaaagcg
    48841 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    48901 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    48961 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    49021 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    49081 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    49141 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    49201 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    49261 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    49321 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    49381 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    49441 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    49501 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    49561 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    49621 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    49681 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    49741 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    49801 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    49861 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    49921 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    49981 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    50041 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    50101 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    50161 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    50221 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    50281 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    50341 agcggcgctt gaggcgggta aacatgtggt cgttgataaa ccctttaccg tgacactgtc
    50401 acaagcgcga gagctggatg cactggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    50461 taaccgtcgc tgggatagcg atttcttgac gctaaaaggt ttactcgcgg aaggcgtgct
    50521 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    50581 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cacatcttct
    50641 tgatcaggcc attacgcttt ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    50701 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    50761 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    50821 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    50881 cgagcgtcta ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    50941 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    51001 ctatgcggct attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    51061 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    51121 tttgtgcctt gcatgaaaaa tggatggtgc aatttgcacc atccattttg ttacttcgcg
    51181 gcgacttttt ctcgcagtgc gcgtttttcc tcagcgctga ggaaagccat ttccagacca
    51241 ttaatctgtg cctggcggat ttgctcgcgg gataacccag cagctggcgc ggcaacggta
    51301 tattcgtgaa tgatatccac tccctgtacg ccgggatcgt cagtgttaat gctggcacga
    51361 atgccatgct caaggaacgt tttcagcgga tgtgcagcca gctctgctac ggtgctggtc
    51421 tgaatattgg aggtcagaca ggattcaata ccaatttgtt gctcggcgag aaaatccatc
    51481 agcgcccgat cttcaatggc ttttacgcca tgtccaatac gttccgcacc cagttcacga
    51541 atcgcctgcc agatgctttc cggcccggca gcttcgcctg catggacggt aatatgccag
    51601 cccgcatcac gcgcgcggtt gaagtgagaa aggaacagac ttcccgggaa accaagttca
    51661 tcaccggcta aatcaagtgc ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct
    51721 tgctgacagg cggcttcgcc gaaggtccgg ctcataatgc cgataagctt cgcctgcaca
    51781 ccaaaggtgc ggcaaccttc acgtacgcca tcgatcaccg cttcgacaac acccgctaca
    51841 ggcagctgat gtgccattgc catgtagcct ggtgaaaaac gcagctcgac atagtgcagg
    51901 ccgtgacggg ctgcatcttc aatgttttca aatgccacgc ggcgacaggc atcaagagag
    51961 gcgagaactt taacgcccca gtcaagtttg gtcagaaagc tcaccagatc gggttcgttg
    52021 gcaatgacct gaacgtgggg aatcagtgtt tccagggatt gtgcaggaag cgagatatta
    52081 tactggcggc caagttcaag aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg
    52141 atatcagtta atggcagggt ggtatcaatc atggtcgcac tctttttctg aatataaaag
    52201 tgcgcaccat tataaaaaca aagcagggta aaaatccacg attgcgcaac gattttttac
    52261 gttgcgcaat tggggttagc gaacagcgcg gatggcgttt attaatccag ccacaccttt
    52321 ttccagtttc gaacgtgggc agccggcatt gagacggaca aaaccacgac cttcttcacc
    52381 ataggtatac cccggcatga tcgcgacttt ttcttgctcg ataagcgctt tttgcaacgc
    52441 gttgtcgtca atattcaacg gacgtagatc aagccaggcc agataagtgg attgcgggat
    52501 ctgccagttg agttcaggaa acgcggcgtt cattttatct gagatatacg tcaggttatc
    52561 tttcagatag acgcgtaagg catccagcca cggtgcacct tgctgatagg cggcgatatg
    52621 ggcagttaac gccagtaccg aaggggaaga aagcccatca cgtcctttca gtgccgataa
    52681 ataggcatca cggctgctgc tattttctat aatcccgtaa gcaccggtca gcgcgggaat
    52741 attgaaactt ttcgagcccg acgtcagcaa cgcccagtct ccccgcgcca cattactcca
    52801 gggaatatgc ggctgctcgc cccagaccat atccatatgg atttcatcgg aaataacccg
    52861 cacaccatga cgctcgcaca ggtcagccat gatctccagc tcatcgcacg tccacacttt
    52921 cccggtagga ttctgtgggc tacacaggag cataatttta cattctggtt tcgccaacac
    52981 ggcttccagc ttgcccatat cgcaaaacca gtcgtcagcc tgcttctcta aagcaacggg
    53041 cattactgtg cgctggttac cttcaatggc cttgtaaaat gcgtcatagg cgggcgtgtg
    53101 gatcaccacg ccttcacctg tttcagacca ctgacgaatc agttctgaaa ccatatagat
    53161 gacagaaggg ccatacacca ccgtctgcgt atcaattgcg gtgtaatgct gggtggaaaa
    53221 ccagtgggca atagccgcga gaaactcatc gtttttccag cggctgtagc caaatacgcc
    53281 gtgcatcagg cgctgattca gcgcctcgat aatgcagggg gcagtggcaa aatccatgtc
    53341 tgaaatcgtg aacggtaaca ggtcagcagt gccgaaacgg tcagcgacat aatcccactg
    53401 tgtacaccat gtgccatgac gatccacgac ctttgaaaaa tcgaacatat cttatcctta
    53461 tgcccggaca gtatgcatca gaccggccat ttcatctttt actgactgaa cttgtggccc
    53521 gataacaacc tgcaggttat gttgattaag ttgtactacg ccaattgccc gattgtcctt
    53581 cagtgcctgc acattaacaa gcgacatatc tttcacagac aaacgcagac gggtaatgca
    53641 gttatcgagg ctgacaatat tgtcggcacc gcctaatgct tcgaggattg caggaacgtt
    53701 gtaacctgat ttacccggcg caccggcaac ggctttttcg attgagctgg caacttcgct
    53761 atcgcgcccc ggggttttca gattgaagcg ggtgatagcg aaacggaaga tgacgtagta
    53821 aacgacaaac cagattgccg ccaccactgg caccatgtac cacttggttg acagaccatg
    53881 caaaataccg aacaccacga agtcgatgat attgccgtcg gtattaccga tggtgacgcc
    53941 gagcacagac atgacagtga agccgaggcc ggttaacagc gcgtggatga catacagaac
    54001 tggcgccacg aacaggaaca ggaattccag cggttcggtc gtgccgccaa cgacacaggc
    54061 gatcaggcca gaaatcagca gacctttaat tttatggcga ttttccgggc gcgcgcagtg
    54121 atacatagct aacgctgcac ctggcagacc gccgagaaac gcaggcattt taccttgcga
    54181 aaggaaacgc gtggcgcttt cagaaaaacc gtgagtggtc gggcaactca attgcgcctg
    54241 gaagatggtc agtgcgccgc tgacggtttg accgcagact tcctgcgtgc cgcctgcgtc
    54301 ggtaaagcga attaatgcca ccagaatgtg atgcagacca aacggcaaca gcagacgttc
    54361 accggtacca aacagcatcg gtccgaaatc acccgcgctg tttatcatat ggcccaagcc
    54421 gctaataccc atggcgaaaa tcggccagac taatggaatc accaggccga taaggcccat
    54481 caccagcgag gagataattg gtacgaagcg cgtaccgccg aagaatgcca gcgcatccgg
    54541 caggcggata ttatggaaac gctcatgcag catccagacg ataataccgg cgatcaccgc
    54601 accgaggatc ccggtatcga tcgactggat cccaagaatg ctctggatgt tattggcttt
    54661 cagaaccgcg gcatccgtgg ttggcagaat gcctttattg gtcaaccaga agtttaccgc
    54721 gaggttcatt accgcataac cgacgaagcc agcgaatgcc gctacgcctt tattttcgcg
    54781 tgccaggccc agcgggatgg cgatacagaa catgacaggc aggaaactaa aagcaaacga
    54841 gccaatctta ctcatccagg taaagatagc ctgcaacacg gggttgccca ggaccgggat
    54901 cagggttatg acatcatggc tgctaagaga actaccaatg ccgagcataa tgccgcagaa
    54961 cgacaataat gccacgggta acatgaaggt tttgcctaac tgctggaaga actcccacag
    55021 cgtgactttc ggtgctgttt tcgccgtcat aaaacgactc ctcgtagaga acaatctgga
    55081 ttcagtaaat tgcgcgagaa gataaaacgt tttatcaaat tttagtgagg cataaatcac
    55141 attacgcaac gataatagcg ggtataagat aaataaaagg taaaacgttt tatctgtcac
    55201 ataatcaggg agtaggtcat ctgcatggct accgccaaaa aaataaccat tcatgatgtt
    55261 gcgctggctg cgggcgtgtc ggtaagtacc gtttcgctgg tgcttagtgg caaagggcga
    55321 atctctaccg ccacaggaga acgcgttaac gccgccattg aagagctggg atttgtgcgc
    55381 aatcgccagg cgtcggcgct gcgcggcggg caaagcggcg tcattggttt gatcgtccgt
    55441 gatttatctg cgccgtttta cgccgaattg acggccggat tgacggaagc tctggaagcg
    55501 cagggacgga tggttttttt gcttcacggc ggtaaagacg gcgagcagct ggcacagcgg
    55561 ttttcactgt tactgaatca gggtgtggat ggtgtggtaa ttgccggggc tgcaggaagc
    55621 agcgatgacc tgcgacggat ggcagaagaa aaagctatcc cggtgatttt cgcttcccgt
    55681 gccagttatc ttgatgatgt tgatacggtt cgcccggaca acatgcaggc tgcacagttg
    55741 ttgacggagc atctcattcg caatgggcat cagcggatcg cctggctggg agggcaaagt
    55801 tcctcattaa cccgtgcaga acgggtgggg ggctattgtg caactctact aaaatttggc
    55861 ctgccgtttc acagcgattg ggtgctggag tgcacatcca gccagaagca agccgcggaa
    55921 gctatcacgg cgcttttacg tcataacccg accatcagtg ctgtggtttg ctataacgaa
    55981 actattgcga tgggggcatg gtttggtttg ctcaaagcag ggcggcaaag cggggaaagc
    56041 ggagtcgatc gttactttga gcaacaagtt tcgctggcgg catttaccga tgcgacacca
    56101 accacacttg atgatatacc cgttacctgg gccagcacgc ctgcgcggga acttggtacc
    56161 acacttgcgg atcgcatgat gcaaaaaatc acccatgaag agacgcattc acgcaatctt
    56221 attattcccg cccggctcat tgcggcgaaa taatcctctc tttatctgct atacctggta
    56281 gtgtcctttc ctcaaggtta atgataaaaa atgtggcaca caggcggtgc cttttatttt
    56341 tactgaagtt cacaggaggt ttatgtgttt aattctgaca acctgagact cgacggaaaa
    56401 tgcgccatca tcacaggtgc gggtgcaggt attggtaaag aaatcgccat tacattcgcg
    56461 acagctggcg catctgtggt ggtcagtgat attaacgccg acgcagctaa ccatgttgta
    56521 gacgaaattc aacaactggg tggtcaggca tttgcctgcc gttgtgatat tacttccgaa
    56581 caggaactct ctgcactggc agactttgcc gtcagtaagc tgggtaaagt tgatatcctg
    56641 gttaacaacg ccggtggcgg tggtcctaaa ccgtttgata tgccaatggc agattttcgc
    56701 cgtgcttatg aactgaatgt gttttctttt ttccatctgt cacaacttgt tgcgccagaa
    56761 atggaaaaaa atggcggtgg cgttattctg accatcactt ctatggcggc agaaaataaa
    56821 aatataaaca tgacttccta tgcatcatct aaagctgcgg ccagtcatct ggtcagaaat
    56881 atggcatttg acctgggtga aaaaaatatt cgggtgaatg gcattgcgcc gggggcaata
    56941 ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacaaaaaat gttacagcac
    57001 acgccgatca gacgtctggg ccaaccgcaa gatattgcta acgcggcgct gttcctttgc
    57061 tcgcctgcag ccagctgggt aagcggacaa attctcaccg tctccggtgg tggggtacag
    57121 gagctcaatt aatacactaa cgaaccggta aacagccgta tgtgttgttt accgggatag
    57181 cgtgtgcaat tcatcaacgt ctctgctaaa caactggcag ccaaatcacg gctattggtt
    57241 aaccaatagc tggatgaaaa gcatacgaat agagtgtgcc ttcgcactat tcaacagcaa
    57301 tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta taatggttta
    57361 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
    57421 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatcc
    57481 tgcgccatta gtcccgggac gctctatcac catttcatct ccaaagaagc cttgattcag
    57541 gcgattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
    57601 attcattttg ttgactatat ggtcgagtct attgtctctc tcacccatga agcctttggg
    57661 caacgggcgc tggtggttga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
    57721 atgcttaaaa ataagcatat gacgatcact gaatttgttg cccagcggat gcgtgatgcc
    57781 cagcaaaaag gcgagataag cccagacatc aacacgtcaa tgacttcacg tttactgctg
    57841 gatctgacct acggtgtact ggccgatatc gaagcggaag acctggcgcg tgaagcgtcg
    57901 tttgctcagg gattacgcgc gatgattggc ggtatcttaa ccgcatcctg atcctctcat
    57961 ttcgcgggct ggtgataact gtgcccgcgt ttcatacttt aatttccctg tacaaaatac
    58021 aatctagccg ggatcggaga attcccccca aaaatattca ccgtagccat atgtcatgag
    58081 agtttatcgt tctcaagatg ctcgaacgaa cgttcgattg cttattttat ggcttctgtc
    58141 aacgctgttt taaagattaa tgcgatctat atcacgctgt gggtattgca gattttggtt
    58201 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
    58261 aactgtgtgc aacacagaat tggttaacta atcagattaa aggttgacca gtatttttat
    58321 ttgaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
    58381 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
    58441 tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
    58501 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagtcttt
    58561 ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
    58621 ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
    58681 gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg
    58741 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga tgaaaacggc
    58801 aagaaaaagc agtcttactt ccatgatttc tttaactatg ccgggatcca tcgcagcgta
    58861 atggtctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgttgcg
    58921 caagactgta accacgcgtc tgttgactgg caggtggtag caaatggtga tgtcagcgtt
    58981 gaactgcgtg atgcggatca gcaggtggtt gcaactggac aaggtaccag cgggactttg
    59041 caagtggtga atccccacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
    59101 acagccaaaa gccagacaga gtgtgatatc tacccgctgc gcgtcggcat ccggtcagtg
    59161 gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
    59221 cgtcatgaag atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac
    59281 cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
    59341 gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct
    59401 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
    59461 tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag
    59521 ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
    59581 gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa
    59641 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
    59701 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa
    59761 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
    59821 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
    59881 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    59941 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
    60001 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac
    60061 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
    60121 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
    60181 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctat
    60241 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac
    60301 tggtgcgggt attcgatgcc ttcgccgacg tctttgccgg acgagtggtg gacagtgtga
    60361 atacccgctg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
    60421 tcagcgtgct ggtattctgg gtaccgaccg actggagcca tagcagcaaa gtagtgtatg
    60481 catatttgac ctacatgggc ctcgggcttt gctacagcct ggtgaatatt ccttatggtt
    60541 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg
    60601 ggattgccgc ttcattaacc tttgtctgcc tggcatttct gataggaccg agcattaaga
    60661 actccagccc ggaagagatg gtgtcggtat accatttctg gacgattgtg ctggcgattg
    60721 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgagaatgtg gtacgtatcg
    60781 tggcgcagcc gtcattgaag atcagtctgc aaaccctgaa acggaatcgc ccgctgttta
    60841 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt cgcggtcagc gcctcgtcgt
    60901 tgttctacgt gcgctatgtg ttaaatgata ccgggctgtt cactgtgctg gtactggtgc
    60961 aaaacctggt tggtactgtg gcatcggcac cgctggtgcc ggggatggtc gcgaggatcg
    61021 gtaaaaagaa taccttcctg attggcgctt tgctgggaac ctgcggttat ctgctattct
    61081 tctgggtttc cgtctggtca ctgccggtgg cgttggttgc gttggccatc gcttcaattg
    61141 gtcagggcgt tactatgact gtgatgtggg cactggaagc tgataccgta gaatacggtg
    61201 aatacctgac cggcgtgcga attgaagggc tcacctattc actattctca tttacccgta
    61261 aatgcggtca ggcaatcgga ggttcaattc ctgcctttat tttgggatta agcggatata
    61321 tcgccaatca ggtgcaaacg ccggaagtta ttatgggcat ccgcacatca attgccttag
    61381 taccttgcgg atttatgcta ctggcattcg ttattatctg gttttatccg ctcacggata
    61441 aaaaattcaa agaaatcgtg gttgaaattg ataatcgtaa aaaagtgcag cagcaattaa
    61501 tcagcgatat cactaattaa tattcaataa aaataatcag aacatcaaag gtgcaactat
    61561 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
    61621 ttatgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaacgaatt
    61681 gcgcagggct gataagccca gtgctggcgc gggaagagat atttacgcat gggtacaggg
    61741 aggattgctc gatttcaata gtggttatta ttccaatatt attggcgttg aaggcggggc
    61801 gtattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
    61861 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaacccagtg aaaatagcct
    61921 gcttaaatta ggtcgcttcg ggacggatta tagttacggt agcttacctt atcgtattcc
    61981 gttaatggct ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
    62041 ttgggcgtta acgccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
    62101 gaccaattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
    62161 cgataaacat cgcgcacgtt cttttttagc cgccagttgg catgatgata ccagtcgcta
    62221 ttctctgggg ggatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
    62281 aggcataccg ctcgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
    62341 actcgaaggt ttaagtcgta ataccagcca gcccaatgat acggcgttgg ttagtggaca
    62401 agtgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
    62461 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
    62521 taaccgtgaa ggaatgcagt cctggcaatt gggcgtcaac tatcgtttaa cgccgcaatt
    62581 tacactgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
    62641 gattgaaggc gcgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
    62701 agggatgaat ttctttcttg ctgccgataa agggcgggaa aagcgcgatg gcagtacgct
    62761 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
    62821 gtaaaaaata acgccggaga gaaaaatctc cggcgttttg tctgtgaatt actgctgcgg
    62881 aatagcgggt acgaccggaa catttaatgc cggcattgca aacataccaa cgaaatcttc
    62941 cagtggcatt ttctgcccat ttaacgttat ctgaccgtta gcatattgca ggctggtggt
    63001 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
    63061 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
    63121 ctgagtcata aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
    63181 aaccgagcgg tccacttcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
    63241 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
    63301 tgcgatagta atcaccggat cgcctttcaa catcagcggc agggcgctaa agaaggcttc
    63361 cgtcactttc tcctgataaa gtgcgggatt gttggcaatt tctggctgtg ccagcagcgc
    63421 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcaccat caatctggcc
    63481 gacttttaaa gtcagcttgc cgctgcccag atcctgattc tgtaccttca ggctgtttag
    63541 cgagtaatcc agttggctat tgatcgtttt accatcattg accagatccg atttaccgct
    63601 gatctccatg ccttccagca gtgccagttc tttgccttcc accgaaatgg tcattttttc
    63661 cagtgacagt ttttggtttc ctacgcgctc accaaaactt gccagcgtgc tggaaccgtc
    63721 ggttttcaga ttattaaagg tcaactgcac tttctggttg tattcgttaa ctgcgtctat
    63781 ccgaccactt tgcgcctccc cggaaaggga gatagctttg ccgtctctgt ccgcatttaa
    63841 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggctt
    63901 gagcgaaata tcggaactgg aatcaccgct gtaaccaatg cgcgagttaa tctcaaaagg
    63961 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
    64021 ttgaattgat gccatcgacg ggatcaggtt cagtttttta agctgggcaa gcgggaaggg
    64081 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggattttc
    64141 tttcccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
    64201 gttttgataa ctcacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
    64261 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
    64321 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
    64381 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
    64441 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
    64501 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
    64561 cacccggttt aagctgtaac tgctgagaac ctttccacaa cgttgcatcg ccttcgacgc
    64621 agaacaaaat ggcggcactc tgctggctaa tggtggtttc tttatcacta aggtcatgca
    64681 gcgagaaggc aaaatcatcc actggaatcg ggaagtccag ttctgcacct tgtttcaccg
    64741 gctgggtcaa caactggtta gccggtttgg cttcgaattt cacattggca accagttccg
    64801 gaatatcaat gtacttaggc gtcagacccg cacgcagcac gttatcggag ttcgccatca
    64861 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
    64921 cgccaggatt caatttcacc acattcagta acagcgggga gaacaggccg ctgtcttccg
    64981 ggtaaaattc agaaattaaa cgaatcgttt gccacggttc accctgctga ctgtctagcg
    65041 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggctgg
    65101 cgaacagttc gcttaaacgt tcggcattag gctgttgtaa aaagtgagca attgccggat
    65161 gtgcacctgc gactggctgg agtagggaga caatctcgga aaactcacga aacgcgttca
    65221 tcgcaaggaa aggcgtcagc gcaaaaacca gctccggctt gtggttagga tctttatagt
    65281 tacgctcggc ggcatccatc gggatacctg cggcattttc tttggcaaaa ccgatttcag
    65341 aattgcgttt gtttggatga acctgaatgg agagcggctg tgctgcgcat aatactttga
    65401 acaggaaagg cagttcgcca aagcgtttgg caacggcctc tccgagcaga gtcgatttat
    65461 tactctcaat cacatcacgc agtgaaacga tatctccggc ggcattctgc acccgtgaac
    65521 tgcttttcgg atgtgcgccc atccacagct cggccatcgg ctggctggac ggattttcca
    65581 taccataaag ttcagtcaac gccgttttgc tgccccaggc atagttttgc actgagttaa
    65641 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccgct attaaagcaa
    65701 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
    65761 aaaagtgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
    65821 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcaggc
    65881 tccttttcca ctcaaaaaag atgatactga gtattacctg ctaaccagcg aacacgttag
    65941 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca cccgaagcgt taactctgtt
    66001 ggcgcgtcag gcatttcatg atgcgtcatt tatgctgcgt ccggctcacc aacaacaggt
    66061 ggccgacatt ctgcgtgacc cggaagccag cgaaaatgat aaatatgtgg cgctgcaatt
    66121 cctgcgcaac tccgacatcg cggcgaaagg cgttctgcca acctgtcagg ataccggcac
    66181 cgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggcggtg atgaagcggc
    66241 gctggcacgc ggtgtctata acacttatat cgaagataat ttgcgctact cgcaaaacgc
    66301 gccgctggat atgtataaag aggtgaatac cggcaccaat ctaccagcgc agatcgatct
    66361 ttatgccgtt gatggcgacg agtacaaatt cctctgtatc gccaaaggtg gcggttcggc
    66421 taacaagacg tatctctatc aggaaaccaa agcgttacta acgccgggga aactgaaaaa
    66481 ttacctggtt gagaagatgc gcacgctggg tacggcggcc tgtcctccgt atcatattgc
    66541 gttcgttatt ggtggaactt ctgcagaaac gaaccttaaa acggtcaaac tggcttccgc
    66601 taaatactat gatgaactgc caacggaagg gaatgagcac ggtcaggcgt tccgcgatgt
    66661 ggaactggaa aaagaattgc tgatcgaagc gcaaaatctt ggtctgggtg cgcagtttgg
    66721 tggtaaatac ttcgctcacg acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg
    66781 cccggtcggt atgggcgtct cctgctctgc tgaccgtaat atcaaagcga agatcaaccg
    66841 tcaggggatc tggatcgaaa aactggaaca taacccaggc aaatatatcc cggaagagct
    66901 gcgcaaagcg ggagaaggcg aagcggtgcg cgttgacctt aaccgtccga tgaaagagat
    66961 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
    67021 cgtcggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
    67081 gccgcagtac attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
    67141 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
    67201 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
    67261 gacggatgcc tgtaaaaaac acggcggctt ctaccttggc agtatcggtg gtccggccgc
    67321 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
    67381 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
    67441 aggaaatgac ttcttccagc agatacaact cacacagtgc actcgctgtg tgaaataaac
    67501 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacat ttgttatcaa
    67561 atagtaaata ataagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
    67621 aaacaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggggcga
    67681 ttgatgtccc ggcagataag ctgtggggcg cacaaactca acgctcgctg gagcatttcc
    67741 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
    67801 cagcggcaaa agttaatgaa gatttaggct tgttgtctga agagaaagcg agcgccattc
    67861 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
    67921 ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa cgaagtgctg gctaaccggg
    67981 ccagtgaatt actcggcggc gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
    68041 tcaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcgctgc
    68101 tggcgctgcg caagcaactc attccgcagc ttaaaaccct gacacagacg ctgagtgaaa
    68161 aatcgcgtgc atttgccgat atcgtaaaaa tcggtcgaac ccacttgcag gacgccacgc
    68221 cgctaacact ggggcaggag atttccggct gggtagcgat gctcgagcat aatctcaaac
    68281 atatcgaata cagcctgcct cacgtagcgg aactggctct tggcggtaca gcggtgggta
    68341 ctggactaaa tacccatccg gagtatgcgc gtcgcgtagc agatgaactg gcagtcatta
    68401 cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc gctggcgacc tgtgatgccc
    68461 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa atcgccaatg
    68521 atgtccgctg gctggcctct ggcccgcgct gcggaattgg tgaaatctca atcccggaaa
    68581 atgagccggg cagctcaatc atgccgggga aagtgaaccc aacccagtgt gaagcattaa
    68641 ccatgctctg ctgtcaggtg atggggaacg acgtggcgat caacatgggg ggcgcttccg
    68701 gtaactttga actgaacgtc ttccgtccaa tggtgatcca caatttcctg caatcggtgc
    68761 gcttgctggc agatggcatg gaaagtttta acaaacactg cgcagtgggt attgaaccga
    68821 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
    68881 cccacattgg ttatgacaaa gccgcggaga tcgccaaaaa agcgcataaa gaagggctga
    68941 ccttaaaagc tgcggccctt gcgctggggt atcttagcga agccgagttt gacagctggg
    69001 tacggccaga acagatggtc ggcagtatga aagccgggcg ttaatctgca acatacaggt
    69061 gcagccgtgg aatgatcaaa cgaagcggct gcgcctgtgg tttataacgg tgctgcacat
    69121 tgtcggcatc gtaatttaac aactcaccaa cgtccggcac gcccgcgcca ttatcccgat
    69181 taatcagtgc aatcagtggt gtagggcagg cgtgttggac ttgtttttga tctcctttgt
    69241 accagacgcg ggcaatcggc tgcaccttca ccggacgttt gatttttaac ttcgcgttct
    69301 gtggcagggc agcgatatcc tgatactctc gctccagttt tctttgccac tcctcgcgcg
    69361 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
    69421 cacgatgcaa attcttaatg atatgtttat tagcccaacc aaagcgtaaa gtggcggggt
    69481 cgtgcagaac ggtgagcgtg cggtaagcat taagggtgat cagccccggc aaatgacgat
    69541 gcacccattc aaaacgtgcc gcggtgggga gttctgattc aaccgtgacg atatgctcga
    69601 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaggt
    69661 tatcgacctg gtaacacaac acgccaggca gacggacagc ggccttgctg ctgcgatttt
    69721 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
    69781 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
    69841 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
    69901 atgtagccag ctcttgttcc atctggcgaa aggtagtgtt gagtcggtct acgagatcgt
    69961 aacgcgccat aatattaacc actttagtta caacatactt attttatagc acagtcgtgg
    70021 tgacttgacc atcgtgcgcg tagtgtcagg cagaggtaaa ttgcgggatg ttatgccata
    70081 acggccagct aaacgagaag cgagcaccac ccagttcgct ggtgtcacaa ttaaccgtac
    70141 cgcccattgc cagtgctata gagtggacaa ttgccagccc cagcccgcag ccgccggttg
    70201 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
    70261 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tttattcccc gacagtagca
    70321 ggctggtttc aaccgttgaa tggcagtacc gcagggcgtt attgagcaaa ttatccagca
    70381 cgcgctccat taagcgcata tccaacgccg cataatggcc ttgcacgagc gtttttatcc
    70441 gtaccgtttt atcgggcgtt actgcctgaa tatctgccag atgcgttgac agccacaacg
    70501 gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg atcgagtcgg gcataagtca
    70561 gcagctcttc aattaaagct tcaagttgac tgatatcacg attcaacgcc tgggattcgg
    70621 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
    70681 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aatcaaggcg ttgatattgt
    70741 ccgccatctg gttaaatgcg ataccaagtc gttcaaagct cgaaccctca tcaaagtgga
    70801 tacgctcatt gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
    70861 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaata gcaataaaag
    70921 cgatcagggc gatatccagc aatcgcatct gatggagata ataaagataa ggaacaggac
    70981 caactgccag cacgtagtgg ctgcgcggga tacgctgcaa aaacgtgtac tgatcgtcca
    71041 gggcgacaat ttcgccgcca cgcagtcggt gcatggaaat atcatcaaga tggtatttac
    71101 tcagtggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
    71161 cccagtcgtg tggggggatc tcacgcaatt cgctgcgcat cagatacagc gaactgttca
    71221 tcaaatcatc cagcgactgt ttgcccgcgc gttcggcggt aaatttgtac accaacccaa
    71281 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacagtt
    71341 ttttcatcgc ttattcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    71401 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    71461 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    71521 cgcggtccat gatttgcccg gcatgggtag ctaattccca caataattcg aaatcagctg
    71581 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatggga tcgatggtca
    71641 acgtgccgaa atgcagggct ttgtagggag tcagagacgt ttcctgaaga cctttggtca
    71701 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    71761 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    71821 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    71881 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggacttt
    71941 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    72001 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    72061 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    72121 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    72181 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    72241 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    72301 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    72361 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    72421 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    72481 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    72541 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcagctggt aagtaagtaa
    72601 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    72661 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    72721 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    72781 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    72841 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    72901 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    72961 agtggtgtac ctggtttttc cgccatccac gcactggcgt tatgcaaaat agcacgcaga
    73021 ccatgggtgc tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    73081 tcagcctgaa tacactgcgc acctgcctta atcagtccat caatggctgg atagtgcgtc
    73141 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccaggcgag ggcgaggccg
    73201 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggctttacc catcgttatc
    73261 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    73321 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    73381 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gcgcatacag aacaacggat
    73441 aacagcaagt gcattggacc agaggcatac aataaccata agccataaat gcaggcaccg
    73501 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    73561 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    73621 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagcgaggcg
    73681 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    73741 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    73801 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    73861 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac gcccagcgaa
    73921 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    73981 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    74041 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccacacggg tacgccaagt
    74101 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    74161 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    74221 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    74281 atcgattgcc aggtattacc atcgccgaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    74341 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgactgc gcacagccag
    74401 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    74461 ataccgccgt caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    74521 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    74581 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    74641 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    74701 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    74761 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    74821 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    74881 cggtcaggtt attaccacgt tcctgccact ggcgggtgat gtggtaatac ttagcacctt
    74941 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    75001 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    75061 ctttagtgcc gtcaggaagc gtgacagtgt aacgtccgcc ttttgatgac tgggtttcaa
    75121 agaaacgacc gacttcagaa cttggtgatg cggtagtcgc aacacctggg atctcaactt
    75181 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    75241 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    75301 tacgctggtt gccgccctgg ttggcatcga tttgacgaac aatgtagaaa gagtaggcac
    75361 ctttcgcttt tgctgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    75421 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    75481 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    75541 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    75601 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    75661 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    75721 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    75781 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    75841 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    75901 atttttcata tcacattcct taagccaatt ttaatcctgc tcaaatgacc gtctatgctt
    75961 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    76021 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    76081 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    76141 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    76201 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    76261 gagcggcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    76321 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    76381 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    76441 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    76501 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    76561 aaattgcgtt actgaatcct gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    76621 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    76681 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaac atcgccggtt
    76741 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaattactg
    76801 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgcgggtgtt gcaggtctgg
    76861 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    76921 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    76981 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    77041 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    77101 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    77161 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacacc gtgccgggtg
    77221 aaatcttcac tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    77281 tgcctacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    77341 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    77401 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    77461 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    77521 cgtggcgtaa atacgcattg atggcgctgg caatcatcct tttcggttgg ctggcaagcg
    77581 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    77641 attacgtggt gtggaatgta tcgcatgcgc tgcatacacc gttgatgtcg gtcaccaacg
    77701 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccagggc ggctgggtta
    77761 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    77821 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    77881 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    77941 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    78001 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    78061 gtcattggtg gggcaattgg tatccgtctg gcgaagaaag ttgaaatgac cgaaatgcca
    78121 gaactggtgg cgatcctgca tagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    78181 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    78241 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gttcggtggt ggcgttcggc
    78301 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    78361 ctggcggctc tggtcgtttc cttcctgctg ctgattgtat ttgttcgcac ggatagcgtc
    78421 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    78481 ttagtcgcct ccatcggtgg tgcagatatg ccagtggtgg tgtcgatgct gaactcgtac
    78541 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    78601 ggtgcgctgg tcggttcttc gggcgctatc ctttcttaca ttatgtgtaa ggcgatgaac
    78661 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    78721 gatcaggaag tgggcgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    78781 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    78841 cctgtcgctg aaattaccga gaaactgcgc gctcgtggta tcaacgtgcg tttcggtatc
    78901 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    78961 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    79021 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    79081 cctattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    79141 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    79201 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    79261 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    79321 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgttaacg gcacggaaag
    79381 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    79441 tgtggaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    79501 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    79561 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    79621 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    79681 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    79741 actgtcaatt gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    79801 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    79861 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    79921 cgcgttcgga tcaatgtaat gcgccagctg gtcaactgag acgtcaatcc ctacgcgttg
    79981 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    80041 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    80101 gatgatggtc atcaaaatcg acaccgccag tacacgcggc actcgccagc ggaccatgtg
    80161 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    80221 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    80281 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt tttcttcctt
    80341 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    80401 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    80461 accgaggaca attatcatcc gcgatgacga gaagcaacac tgcggataat tgtaatatta
    80521 tggacaattt gttcaggacg ttattttcaa catttaccca tcttcgtacc tcatccacta
    80581 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    80641 ttgccctatg cgcggggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    80701 aaataattct cttgcaggag aaggacaatg tatatttatt ggattttatt aggcctggct
    80761 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    80821 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    80881 gttaaaaaga tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    80941 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    81001 ttaaccaccc tggtcgccgg gattgtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    81061 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    81121 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    81181 caaaatattt ggcttgctct ccctggcggc ggtgctggct gcctttagtg cgctttctca
    81241 ggccgtaaaa gggatcgact tgtctgtcgc ttatgcattg tggggcgggt ttggtattgc
    81301 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    81361 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    81421 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    81481 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    81541 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    81601 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    81661 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcaggat atcccgcctg
    81721 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    81781 taacggcaac ggtgtaatgc cagaaggggg attacgcagc acaatcaagc cgaagtccca
    81841 tggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    81901 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    81961 cacaaaacgc agcgccactg ctttatcggc tttacccttt ggaggtgtca ataaacagtg
    82021 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    82081 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    82141 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    82201 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    82261 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    82321 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    82381 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    82441 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    82501 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    82561 gcagcaggtt ttgccggttg ctgatggctg tgtttcccgg cgtgtttctt cgctgcctgc
    82621 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    82681 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgccggc
    82741 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    82801 gtagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    82861 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    82921 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    82981 ataaattaca gcattgataa taacgtattg tgacttaagg gaaatttagc taccaataat
    83041 agtagtcttg atcgggttaa ctattttact acttacaaag cggtagaata actgcgcatc
    83101 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    83161 cgctagacgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    83221 tggtttgagc aagcaataag tccggacgtg tagttaccgc agtccgggct tatttttcag
    83281 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    83341 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    83401 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    83461 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    83521 gagaagagta acattccggc aaagatcagc cacagcgagt tgaataaggt catcagtaaa
    83581 ccaaacagca taacccccgt cgaaaacaac atcaccggac cgcgcccgta gcgggtggtc
    83641 atggttccgg ctttaggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    83701 accacggcct gactgacatg ccagggggaa agcatcaacc gatagccgat gtaattaaac
    83761 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    83821 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcga
    83881 aaatggcgtg attccggaag gattttccag aacatcaacg ccgaggctag cgcgaaacaa
    83941 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    84001 cgcccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    84061 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    84121 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    84181 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    84241 gcatcggata gggggccagt aaacagcaaa ccaatagcca acatcgccgt ggaaatggac
    84301 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    84361 ggttgcacac aatagagaag tgcgaaggtt gccagtccgg cagagaacag cgccagggtg
    84421 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    84481 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    84541 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    84601 taatatatta ataatctcaa ataag
//
LOCUS       DAZFFI010000008        80837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFFI010000008 DAZFFI010000000
VERSION     DAZFFI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.008"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1589
                     /locus_tag="ACHJ12_000723"
     CDS             <1..1589
                     /locus_tag="ACHJ12_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO4988556.1"
                     /translation="YIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVL
                     LVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEM
                     KDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVD
                     ERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGY
                     RNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWI
                     ARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIG
                     FINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENG
                     LKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDS
                     IKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGY
                     LYQKPMPFSAWDKSGKLVKE"
     gene            1597..2925
                     /gene="dgcI"
                     /locus_tag="ACHJ12_000724"
     CDS             1597..2925
                     /gene="dgcI"
                     /locus_tag="ACHJ12_000724"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4988557.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2972..4297)
                     /gene="rimO"
                     /locus_tag="ACHJ12_000725"
     CDS             complement(2972..4297)
                     /gene="rimO"
                     /locus_tag="ACHJ12_000725"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4988558.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4510..4893
                     /gene="bssR"
                     /locus_tag="ACHJ12_000726"
     CDS             4510..4893
                     /gene="bssR"
                     /locus_tag="ACHJ12_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4988559.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5004..6119
                     /gene="yliI"
                     /locus_tag="ACHJ12_000727"
     CDS             5004..6119
                     /gene="yliI"
                     /locus_tag="ACHJ12_000727"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4988560.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVADFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6116..6742)
                     /gene="gstB"
                     /locus_tag="ACHJ12_000728"
     CDS             complement(6116..6742)
                     /gene="gstB"
                     /locus_tag="ACHJ12_000728"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4988561.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6989..8191
                     /gene="dacC"
                     /locus_tag="ACHJ12_000729"
     CDS             6989..8191
                     /gene="dacC"
                     /locus_tag="ACHJ12_000729"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4988562.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8238..8996)
                     /gene="deoR"
                     /locus_tag="ACHJ12_000730"
     CDS             complement(8238..8996)
                     /gene="deoR"
                     /locus_tag="ACHJ12_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4988563.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9054..9650)
                     /gene="ybjG"
                     /locus_tag="ACHJ12_000731"
     CDS             complement(9054..9650)
                     /gene="ybjG"
                     /locus_tag="ACHJ12_000731"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4988564.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVIIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9935..11167
                     /gene="mdfA"
                     /locus_tag="ACHJ12_000732"
     CDS             9935..11167
                     /gene="mdfA"
                     /locus_tag="ACHJ12_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO4988565.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIQQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11208..11492)
                     /gene="ybjH"
                     /locus_tag="ACHJ12_000733"
     CDS             complement(11208..11492)
                     /gene="ybjH"
                     /locus_tag="ACHJ12_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4988566.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11578..12393)
                     /gene="ybjI"
                     /locus_tag="ACHJ12_000734"
     CDS             complement(11578..12393)
                     /gene="ybjI"
                     /locus_tag="ACHJ12_000734"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4988567.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(12393..13601)
                     /gene="ybjJ"
                     /locus_tag="ACHJ12_000735"
     CDS             complement(12393..13601)
                     /gene="ybjJ"
                     /locus_tag="ACHJ12_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988568.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13685..14221
                     /gene="rcdA"
                     /locus_tag="ACHJ12_000736"
     CDS             13685..14221
                     /gene="rcdA"
                     /locus_tag="ACHJ12_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4988569.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(14396..16081)
                     /gene="ybjL"
                     /locus_tag="ACHJ12_000737"
     CDS             complement(14396..16081)
                     /gene="ybjL"
                     /locus_tag="ACHJ12_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4988570.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16351..16728
                     /gene="ybjM"
                     /locus_tag="ACHJ12_000738"
     CDS             16351..16728
                     /gene="ybjM"
                     /locus_tag="ACHJ12_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4988571.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16758..17015)
                     /gene="grxA"
                     /locus_tag="ACHJ12_000739"
     CDS             complement(16758..17015)
                     /gene="grxA"
                     /locus_tag="ACHJ12_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4988572.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17175..17462
                     /gene="ybjC"
                     /locus_tag="ACHJ12_000740"
     CDS             17175..17462
                     /gene="ybjC"
                     /locus_tag="ACHJ12_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4988573.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17446..18168
                     /gene="nfsA"
                     /locus_tag="ACHJ12_000741"
     CDS             17446..18168
                     /gene="nfsA"
                     /locus_tag="ACHJ12_000741"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4988574.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCFGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18229..19131
                     /gene="rimK"
                     /locus_tag="ACHJ12_000742"
     CDS             18229..19131
                     /gene="rimK"
                     /locus_tag="ACHJ12_000742"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4988575.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19219..19695
                     /gene="ybjN"
                     /locus_tag="ACHJ12_000743"
     CDS             19219..19695
                     /gene="ybjN"
                     /locus_tag="ACHJ12_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4988576.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20047..21159
                     /gene="potF"
                     /locus_tag="ACHJ12_000744"
     CDS             20047..21159
                     /gene="potF"
                     /locus_tag="ACHJ12_000744"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4988577.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTCFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFLIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21254..22387
                     /gene="potG"
                     /locus_tag="ACHJ12_000745"
     CDS             21254..22387
                     /gene="potG"
                     /locus_tag="ACHJ12_000745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4988578.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22397..23350
                     /gene="potH"
                     /locus_tag="ACHJ12_000746"
     CDS             22397..23350
                     /gene="potH"
                     /locus_tag="ACHJ12_000746"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4988579.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            23347..24192
                     /gene="potI"
                     /locus_tag="ACHJ12_000747"
     CDS             23347..24192
                     /gene="potI"
                     /locus_tag="ACHJ12_000747"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4988580.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            24252..24740
                     /gene="ybjO"
                     /locus_tag="ACHJ12_000748"
     CDS             24252..24740
                     /gene="ybjO"
                     /locus_tag="ACHJ12_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4988581.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            24781..25908
                     /gene="rlmC"
                     /locus_tag="ACHJ12_000749"
     CDS             24781..25908
                     /gene="rlmC"
                     /locus_tag="ACHJ12_000749"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4988582.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDVRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(26083..26814)
                     /gene="artJ"
                     /locus_tag="ACHJ12_000750"
     CDS             complement(26083..26814)
                     /gene="artJ"
                     /locus_tag="ACHJ12_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4988583.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(27106..27774)
                     /gene="artM"
                     /locus_tag="ACHJ12_000751"
     CDS             complement(27106..27774)
                     /gene="artM"
                     /locus_tag="ACHJ12_000751"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4988584.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(27774..28490)
                     /gene="artQ"
                     /locus_tag="ACHJ12_000752"
     CDS             complement(27774..28490)
                     /gene="artQ"
                     /locus_tag="ACHJ12_000752"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4988585.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(28497..29228)
                     /gene="artI"
                     /locus_tag="ACHJ12_000753"
     CDS             complement(28497..29228)
                     /gene="artI"
                     /locus_tag="ACHJ12_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4988586.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(29246..29974)
                     /gene="artP"
                     /locus_tag="ACHJ12_000754"
     CDS             complement(29246..29974)
                     /gene="artP"
                     /locus_tag="ACHJ12_000754"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4988587.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(30192..30707)
                     /gene="ybjP"
                     /locus_tag="ACHJ12_000755"
     CDS             complement(30192..30707)
                     /gene="ybjP"
                     /locus_tag="ACHJ12_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4988588.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            30833..31156
                     /gene="ybjQ"
                     /locus_tag="ACHJ12_000756"
     CDS             30833..31156
                     /gene="ybjQ"
                     /locus_tag="ACHJ12_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4988589.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            31153..31983
                     /gene="amiD"
                     /locus_tag="ACHJ12_000757"
     CDS             31153..31983
                     /gene="amiD"
                     /locus_tag="ACHJ12_000757"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4988590.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     RVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(31980..32993)
                     /gene="ybjS"
                     /locus_tag="ACHJ12_000758"
     CDS             complement(31980..32993)
                     /gene="ybjS"
                     /locus_tag="ACHJ12_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4988591.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(33092..34522)
                     /gene="ybjT"
                     /locus_tag="ACHJ12_000759"
     CDS             complement(33092..34522)
                     /gene="ybjT"
                     /locus_tag="ACHJ12_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4988592.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(34533..35534)
                     /gene="ltaE"
                     /locus_tag="ACHJ12_000760"
     CDS             complement(34533..35534)
                     /gene="ltaE"
                     /locus_tag="ACHJ12_000760"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4988593.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYTA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(35571..37289)
                     /gene="poxB"
                     /locus_tag="ACHJ12_000761"
     CDS             complement(35571..37289)
                     /gene="poxB"
                     /locus_tag="ACHJ12_000761"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4988594.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHAEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(37422..38390)
                     /gene="hcr"
                     /locus_tag="ACHJ12_000762"
     CDS             complement(37422..38390)
                     /gene="hcr"
                     /locus_tag="ACHJ12_000762"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4988595.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(38402..40054)
                     /gene="hcp"
                     /locus_tag="ACHJ12_000763"
                     /gene_synonym="priS"
     CDS             complement(38402..40054)
                     /gene="hcp"
                     /locus_tag="ACHJ12_000763"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4988596.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDPGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(40198..41097)
                     /gene="lysO"
                     /locus_tag="ACHJ12_000764"
     CDS             complement(40198..41097)
                     /gene="lysO"
                     /locus_tag="ACHJ12_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4988597.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(41554..42249)
                     /gene="aqpZ"
                     /locus_tag="ACHJ12_000765"
     CDS             complement(41554..42249)
                     /gene="aqpZ"
                     /locus_tag="ACHJ12_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4988598.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            42675..44333
                     /gene="ybjD"
                     /locus_tag="ACHJ12_000766"
     CDS             42675..44333
                     /gene="ybjD"
                     /locus_tag="ACHJ12_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4988599.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLSNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(44330..45286)
                     /gene="ybjX"
                     /locus_tag="ACHJ12_000767"
     CDS             complement(44330..45286)
                     /gene="ybjX"
                     /locus_tag="ACHJ12_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4988600.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            45437..46552
                     /gene="macA"
                     /locus_tag="ACHJ12_000768"
     CDS             45437..46552
                     /gene="macA"
                     /locus_tag="ACHJ12_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4988601.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLLVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            46549..48495
                     /gene="macB"
                     /locus_tag="ACHJ12_000769"
     CDS             46549..48495
                     /gene="macB"
                     /locus_tag="ACHJ12_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4988602.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(48568..48792)
                     /gene="cspD"
                     /locus_tag="ACHJ12_000770"
     CDS             complement(48568..48792)
                     /gene="cspD"
                     /locus_tag="ACHJ12_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4988603.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            48942..49016
                     /gene="yljB"
                     /locus_tag="ACHJ12_000771"
     CDS             48942..49016
                     /gene="yljB"
                     /locus_tag="ACHJ12_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4988604.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            49115..49435
                     /gene="clpS"
                     /locus_tag="ACHJ12_000772"
     CDS             49115..49435
                     /gene="clpS"
                     /locus_tag="ACHJ12_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4988605.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            49466..51742
                     /gene="clpA"
                     /locus_tag="ACHJ12_000773"
     CDS             49466..51742
                     /gene="clpA"
                     /locus_tag="ACHJ12_000773"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4988606.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(52086..52173)
                     /locus_tag="ACHJ12_000774"
     tRNA            complement(52086..52173)
                     /locus_tag="ACHJ12_000774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52137..52139),aa:Ser,seq:gga)
     gene            complement(52427..52645)
                     /gene="infA"
                     /locus_tag="ACHJ12_000775"
     CDS             complement(52427..52645)
                     /gene="infA"
                     /locus_tag="ACHJ12_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4988607.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(52930..53634)
                     /gene="aat"
                     /locus_tag="ACHJ12_000776"
     CDS             complement(52930..53634)
                     /gene="aat"
                     /locus_tag="ACHJ12_000776"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4988608.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(53676..55397)
                     /gene="cydC"
                     /locus_tag="ACHJ12_000777"
     CDS             complement(53676..55397)
                     /gene="cydC"
                     /locus_tag="ACHJ12_000777"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4988609.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(55398..57164)
                     /gene="cydD"
                     /locus_tag="ACHJ12_000778"
     CDS             complement(55398..57164)
                     /gene="cydD"
                     /locus_tag="ACHJ12_000778"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4988610.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDIIWVMQDGQIIEQGR
                     YAELSVAGGPFAKLLAHRQEEI"
     gene            complement(57287..58252)
                     /gene="trxB"
                     /locus_tag="ACHJ12_000779"
     CDS             complement(57287..58252)
                     /gene="trxB"
                     /locus_tag="ACHJ12_000779"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4988611.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            58797..59291
                     /gene="lrp"
                     /locus_tag="ACHJ12_000780"
     CDS             58797..59291
                     /gene="lrp"
                     /locus_tag="ACHJ12_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4988612.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            59426..63493
                     /gene="ftsK"
                     /locus_tag="ACHJ12_000781"
     CDS             59426..63493
                     /gene="ftsK"
                     /locus_tag="ACHJ12_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4988613.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQSIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            63648..64259
                     /gene="lolA"
                     /locus_tag="ACHJ12_000782"
     CDS             63648..64259
                     /gene="lolA"
                     /locus_tag="ACHJ12_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4988614.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            64270..65613
                     /gene="rarA"
                     /locus_tag="ACHJ12_000783"
                     /gene_synonym="mgsA"
     CDS             64270..65613
                     /gene="rarA"
                     /locus_tag="ACHJ12_000783"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4988615.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            65704..66996
                     /gene="serS"
                     /locus_tag="ACHJ12_000784"
     CDS             65704..66996
                     /gene="serS"
                     /locus_tag="ACHJ12_000784"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4988616.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            67235..69679
                     /gene="dmsA"
                     /locus_tag="ACHJ12_000785"
     CDS             67235..69679
                     /gene="dmsA"
                     /locus_tag="ACHJ12_000785"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4988617.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            69690..70307
                     /gene="dmsB"
                     /locus_tag="ACHJ12_000786"
     CDS             69690..70307
                     /gene="dmsB"
                     /locus_tag="ACHJ12_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4988618.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            70309..71172
                     /gene="dmsC"
                     /locus_tag="ACHJ12_000787"
     CDS             70309..71172
                     /gene="dmsC"
                     /locus_tag="ACHJ12_000787"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4988619.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(71208..71834)
                     /gene="ycaC"
                     /locus_tag="ACHJ12_000788"
     CDS             complement(71208..71834)
                     /gene="ycaC"
                     /locus_tag="ACHJ12_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4988620.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            72149..73297
                     /gene="ycaD"
                     /locus_tag="ACHJ12_000789"
     CDS             72149..73297
                     /gene="ycaD"
                     /locus_tag="ACHJ12_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988621.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            73507..74937
                     /gene="ycaM"
                     /locus_tag="ACHJ12_000790"
     CDS             73507..74937
                     /gene="ycaM"
                     /locus_tag="ACHJ12_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4988622.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(74938..75846)
                     /gene="ycaN"
                     /locus_tag="ACHJ12_000791"
     CDS             complement(74938..75846)
                     /gene="ycaN"
                     /locus_tag="ACHJ12_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4988623.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            75946..76536
                     /gene="ycaK"
                     /locus_tag="ACHJ12_000792"
     CDS             75946..76536
                     /gene="ycaK"
                     /locus_tag="ACHJ12_000792"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO4988624.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(76618..77358)
                     /gene="pflA"
                     /locus_tag="ACHJ12_000793"
     CDS             complement(76618..77358)
                     /gene="pflA"
                     /locus_tag="ACHJ12_000793"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4988625.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(77550..79832)
                     /gene="pflB"
                     /locus_tag="ACHJ12_000794"
     CDS             complement(77550..79832)
                     /gene="pflB"
                     /locus_tag="ACHJ12_000794"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4988626.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(79887..80744)
                     /gene="focA"
                     /locus_tag="ACHJ12_000795"
     CDS             complement(79887..80744)
                     /gene="focA"
                     /locus_tag="ACHJ12_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4988627.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        19901 a        19976 c        21245 g        19715 t
ORIGIN      
        1 agtatattgc aaagacatca gcagtgccaa ataatgcatt gttcgaaatc tatttttatt
       61 atcctggcgg taatttattg aacgcatcag ataaactttt ttatctaccg tttgctttca
      121 ttattatcgt gttgctggtg gtatatttca tgaccactcg tgtgttccgt cggcaatttt
      181 ctgaaatgac cgagctggtt aatacgctgg cgtttttgcc cgactcaacg gatcaaatcg
      241 aggctctgaa aattcgcgaa ggcgatgcga aagagattat cagcatcaaa aattcgatcg
      301 cggaaatgaa agatgccgaa attgaacggt caaataaatt gctctcactg atctcttacg
      361 atcaggaaag cggttttatt aaaaatatgg cgattattga gtccaacaat aatcagtatc
      421 tggctgtggg gatcatcaaa ctgtgtggtc tggaagccgt ggaagcggtg tttggtgttg
      481 atgaacgcaa taaaatcgtc aggaaattgt gtcagcgaat tgccgagaaa tatgcgcaat
      541 gctgcgatat cgtgacattc aatgccgatc tctatttact tctgtgtcgg gaaaatgtac
      601 agacatttac ccgtaaaata gcgatggtaa acgattttga cagcagcttt ggctaccgca
      661 atctgcgcat ccataagtct gccatttgtg aacctttgca gggggaaaac gcctggagtt
      721 acgcagaaaa actgaaactg gcgatttcca gtatccgtga ccatatgttc tccgagttta
      781 ttttttgtga tgacgcgaaa ctcaacgaaa tagaagagaa tatctggatt gcgcgtaata
      841 ttcgccatgc aatggaaatt ggcgaactat tcctcgtcta tcaaccgatc gttgatatta
      901 acacccgcgc cattctgggc gcggaggcgt tgtgccgttg ggtttctgcg gagcggggga
      961 tcatttcacc gctgaagttc attaccattg ctgaagatat cgggtttatc aatgagctgg
     1021 gttatcagat tattaaaacg gcaatgggtg aattcagaca ttttagtcag cgtgcgtcgc
     1081 tgaaggatga tttcttactg catattaatg tttcgccctg gcagttaaac gaaccgcact
     1141 ttcatgagcg ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg
     1201 ttgagatcac tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac
     1261 aactgcgtaa acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc
     1321 tgaaacgttt ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg
     1381 atattttcgg tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc
     1441 ggatcccggt gattgcggaa ggcgtagaga gcgaagacgt tgcgcgcgaa ttaattaaat
     1501 taggatgtgt tcaggctcag gggtatctgt accagaaacc catgccattc tccgcctggg
     1561 ataaaagtgg aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta
     1621 cttacagtca gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact
     1681 caaatggtaa aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg
     1741 gtggcaaata ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat
     1801 cccacggagg aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc
     1861 tcttatttgt acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag
     1921 gagttcgaga aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa
     1981 aatacagggt ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact
     2041 cacaatcgta gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg
     2101 ctggcgatgg acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc
     2161 ggtgatgaat ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc
     2221 gcctatttgc cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc
     2281 tgtacattga ttattttctt tgccgcattg gtggcagtga tatcgggtac cagttgcctc
     2341 tatctggtac gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac
     2401 cattttgaac gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc
     2461 tacagtatgt ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc
     2521 tttgacgaag atttaaaagc gctgccggaa aaaggcggtt atttgtgcct gtttgacgtc
     2581 gataaattca aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg
     2641 aaagtggtga aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc
     2701 ggcggtgacg aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt
     2761 ctaaaagaaa tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta
     2821 gcgcattcaa atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg
     2881 tataagagta agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc
     2941 cggacaaatg attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact
     3001 catcggcgtg ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat
     3061 tgagataaac cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt
     3121 cttcgtccac ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct
     3181 cggcggaaat ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt
     3241 ccggaacctg gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc
     3301 agccaacgcg atccagacgc gcttctttca ggaagtctag tagcatctgg aaatcttctt
     3361 ctgtctcgcc agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt
     3421 cgcgccactg tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt
     3481 tgagaatgcg cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt
     3541 ctgccatcag tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg
     3601 tccagatccc cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct
     3661 cgccgttgtg gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg
     3721 agatcaccag aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt
     3781 cgccaatcgg acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc
     3841 ggtgattaca gccttctgaa attttcagat aggcataatg acgcggtgtc agtttcacac
     3901 cttgttctgg caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa
     3961 cgtgctccag aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga
     4021 cttcgcggat ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt
     4081 cattcaacgc ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc
     4141 aggtgttgac gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag
     4201 tgcgtagttc ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa
     4261 caaagccgat tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca
     4321 ggaaaggtca gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt
     4381 cgctggcgga ggcgaaggaa atgtaaattt tgttaatttg gcgtgaagaa ttgatcctgg
     4441 acagcatttt gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag
     4501 gatcgaagta tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca
     4561 cgcgttgacc tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc
     4621 gaaagcaatc atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg
     4681 ctgcggttga atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa
     4741 gcattagcga cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt
     4801 accgtcaacg ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc
     4861 ctgcagaagc acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc
     4921 cttcatgttt aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa
     4981 gccagagtca atccggaggc gttatgcatc gacaatcctt tttccttgtg ccccttattt
     5041 gtctttcttc cgctctctgg gcggctcctg caacggtaaa tgtcgaagta ctgcaagaca
     5101 aactcgacca tccctgggca ctggcctttt tacccgataa tcacggtatg ttaatcactc
     5161 tgcgcggcgg cgagttgcgt cactggcaag caggaaaagg attatctgcg ccgctttccg
     5221 gagttccgga cgtttgggcg cacgggcagg gcggcctgct ggacgtggtt ttagcgcctg
     5281 attttgctca gtctcgccgc atctggttaa gttattccga agttggcgat gatggcaaag
     5341 ccggaactgc tgtgggttat ggccgcttaa gtgatgatct ctcaaaagtg gccgacttcc
     5401 gcaccgtctt ccgccagatg ccaaaactgt ctaccggcaa ccattttggc gggcggctgg
     5461 tattcgacgg taaaggttat ctttttattg ctctgggcga aaacaatcag cgcccgacgg
     5521 cgcaggatct ggataaatta cagggcaaac tggtgcgtct gaccgaccag ggcgaaatcc
     5581 cggatgataa tccttttata aaggaatccg gtgcgcgcgc cgagatctgg tcttatggca
     5641 ttcgtaatcc gcaaggaatg gcgatgaatc cgtggagtaa tgcactgtgg ctgaatgaac
     5701 atggcccgcg cggtggtgat gaaattaata tcccgcaaaa aggcaaaaac tacggctggc
     5761 cgctggcaac ctggggaatc aactattcag gctttaagat accggaagcg aaaggggaga
     5821 tcgtcgccgg gaccgagcaa cctgtttttt actggaaaga ttcgcccgct gtgagcggca
     5881 tggccttcta taacagcgat aaattccccc agtggcagca aaaattattt ataggcgcgc
     5941 tgaaagataa agatgtcatt gtgatgagcg tcaacggcga caaagtgaca gaagatggcc
     6001 gtattttaac ggacagaggg cagcgaattc gtgatgttcg cactggaccc gacggttatt
     6061 tatacgttct caccgacgag tccagtgggg aattacttaa agttagccca cgcaattagc
     6121 taacgggaat catcaccact ttgcgcactg cggggcgttc agtgagttgc tgataccagc
     6181 gttgcagatt tggacgcggt gtccaggtca ggccaacgtt aaacaaattg tagatgaacg
     6241 gtgcgatagc gatatcgccc acaccaaact cgtcgccaga gaaccatttt acttttgcca
     6301 gttccgcatc gagcagggca aacagggcgt cgcactcttt gcaactggca tcaatggcgg
     6361 cctgatcgcg ctcttcgggt ggtgttctga ctaatcccat caggatcccg cgatgagcat
     6421 tactgagtgt ctggtttgcc cagtccatcc atttttccgc ttccgcacga cgtgccggtg
     6481 agtcgatcca caggcgtttt tgcccgtact gtgccgccag atagcggaca atggcgtttg
     6541 attcccaaag aatgagatca ctttcgtcgt cacgcaacaa cggcaccagc ccgttagggt
     6601 tcatcgccag aaaatcagca tcgtgattta tcccaaactc acggcccgcg agaatttgct
     6661 cataaggtag ttccagttct tcgagcgtca gcaatacttt ttttacgttg gttgaattat
     6721 tccgacccca cagcgtaatc atactcaccc cttttccgac aggcagccac agactgaaca
     6781 ggactccgat ggtgaagtaa agttaacttt taagcaacag ctggcaaaaa aatgacacca
     6841 gaagcagcga agcggcagga tattgcataa actttaaaaa ctttaccaac ttacggtttc
     6901 tttaagtttg tgtgtgcgtt attaatcacc gaacttatca tacggcgata taacgtattt
     6961 tttttgaatg gatactcggg tggcatttat gacgcaatac tcctctctcc ttcgtggtct
     7021 tgcagcgggt tctgcatttt tattcctttt tgccccaacg gcattcgcgg ccgaacaaac
     7081 cgttgaagcg ccgagcgtgg atgcgcgtgc atggatttta atggattacg ccagcggtaa
     7141 agtgctggca gaaggcaatg cggatgagaa actggatccc gcgagcctga ctaaaatcat
     7201 gaccagctat gtggttgggc aggcgcttaa ggccgataag attaaactca ccgatatggt
     7261 gacggtcggt aaagatgcct gggcgacggg aaatccggca ctgcgtggtt catcggtaat
     7321 gttcctcaaa ccgggcgatc aggtttcggt ggcagacttg aacaaaggtg tgattatcca
     7381 gtccggtaat gacgcctgta ttgcgctggc tgattacgtt gccgggagcc aggagtcatt
     7441 tattggtctg atgaatggtt atgccaaaaa actgggtctg accaacacta ccttccagac
     7501 ggtgcacggc ctggatgcgc cggggcagtt cagcaccgcg cgcgatatgg cattgctggg
     7561 taaagcattg atccacgatg tgccggaaga gtacgccatt cataaagaga aagagttcac
     7621 cttcaacaaa attcgtcagc ctaaccgtaa ccgtctgctg tggagcagca atctgaatgt
     7681 tgatggcatg aagacaggaa ccacggcagg cgcgggatat aatctggttg cttcggctac
     7741 ccagggcgat atgcgtttaa tctccgtagt gttgggggcg aaaaccgacc gtatccgttt
     7801 taatgagtct gagaaattat tgacctgggg tttccgcttc tttgaaaccg tgacgccaat
     7861 taaacctgat gccacctttg tgactcagcg cgtctggttt ggtgataaga gcgaagtgaa
     7921 tctcggggca ggtgaagcgg gctcagtgac cataccgcgt gggcagctga aaaacctgaa
     7981 agcgagttat acgttaacgg aaccgcagct taccgcaccg ctgaaaaaag gtcaggttgt
     8041 cgggaccatt gatttccagc ttaacggtaa atccattgag cagcgtccgc tgatcgtgat
     8101 ggaaaatgtg gaagagggcg gattctttgg tcggatgtgg gatttcgtga tgatgaaatt
     8161 ccatcagtgg ttcggcagct ggttctctta atcttctgat aaccggatgg cgcgaaacgt
     8221 catccggtta tacgtcatta atacatcaac ttaatgcgct gcgtctgcgc gtacttcaca
     8281 tactcatctt ccgggcaaca atcgctcacc acaatatcaa agcgtttcag gtcacccatg
     8341 cgcgccggac gcaccttgcc aaatttactg tggtcgacaa ccagcacatg cttttgcgcc
     8401 atcgacatgg cccagtgttt taccggcaac tcttcaagat taaaacaggt agcgccttta
     8461 ctgacatgca cgcccgccgc agaataaaaa gcgatatccg ggcaaaaatt attcagcgtt
     8521 tgctgaaaat cgatgggttt gaaaatggcg ttgctggcgt gaaattcacc accgcacaga
     8581 aatgcgcggc aatggggttt ctctttcagc gccagaaagg tatttagcga ataacaaacg
     8641 gcggtgaaag gtatttcatt atcaatcgct tcaataatcc acggcgtggt ggtgccacag
     8701 tcaaaaaaga gggtctgatc gggttctacc agcgtcgcag ccagttttgc cgcccggcgt
     8761 ttttcttcca ccaggcggga tttttgatcg cttaacagat aatggctggc actgcgcggt
     8821 tccagaacaa tatagccgcc gagcaaaacg acgggcgcac tgtggttgtt cagatcgcga
     8881 cgaatcgtca tctccgaaac cccaagcagg gcggcggcgt ctttaagatg taacttatcg
     8941 ctgcgtttta attcttgcag cagctgcccg atacgctctt cgcgacgtgt ttccataatc
     9001 cctctgaata gttattgaag cgagccgctc aatactacac tttttagcag agatcagtca
     9061 cgcacccagc ctttgcggat cggtaatgca aaacagaagc gataccacga ttgcagacgt
     9121 tgatagagtt tatgccccat cgcttgccag ataatctggg cactaaggca gccaatcata
     9181 cctgccagca atccaccgag catatccagc ggccagtgga cgccaagata aacgcgcgac
     9241 caggcaatga tgacggccag caccattaaa agtgagccgg accacaggcg atgccagcat
     9301 aaaaatgcca gtgcaaaggt gaaaatcacc gtaccgtgat cgcttgggaa tgagtcatcc
     9361 gccgcatgat gcaggaagtt atagccgata ttttcgacaa agggtcggtc gtgcggaaaa
     9421 agatgtccca tcgtccagga cacaaacagg ctgaccgcca gcgcgatagc gatttttatc
     9481 accagttgcc gttgtgctgt aagcccccac aaccaaagta ccgcggccag cagcggcacc
     9541 acggtaatca aatctttagc aataaaaatc gccaacgaga tcatccacgg agccgagtct
     9601 ggcgtcgcgt taataagaga gaatagagag agattcaaat tttccagcat aacttcccga
     9661 cgcaaagtga ttaaaaggga gccaatacag gcaagtcgtt gagaataaag tgcaggttaa
     9721 actgggtaaa gcggcatcgt cttatttccc tcaagcggcc tgtttacggt gggtgattgt
     9781 aacgggcata ggttaaataa aacttaaaga aagcgtagct atactcgcaa taatgtaaga
     9841 atgtgcttaa ccgtggtttc agctacaaaa ttcgctttct cgttagctgc gcttttatta
     9901 aactctgcgc gattattatt ggcgaagaaa ttgcatgcaa aataaattag cttccggtgc
     9961 caggcttgga cgtcaggcgt tacttttccc tctctgtctg gtgctttacg aattttcaac
    10021 ctatatcggc aacgatatga ttcaacccgg tatgttggcc gtggtggaac aatatcaggc
    10081 gggcattgat tgggttccta cttcgatgac cgcgtatctg gcaggcggga tgtttttaca
    10141 atggctcctg gggccgctgt cggatcgtat tggtcgccgt ccggtgatgc tggcgggtgt
    10201 ggtgtggttt atcgtcacct gtctggcaat attactggcg caaaacatac aacaattcac
    10261 cctgttgcgc ttcttgcagg gcataagcct ctgtttcatt ggcgctgtgg gatacgccgc
    10321 aattcaggaa tccttcgaag aggcggtttg tatcaagatc accgcgctga tggcgaacgt
    10381 ggcgctgatt gctccgctac ttggtccgct ggtgggcgcg gcgtggatcc atgtgctgcc
    10441 ctgggagggg atgtttgttt tgtttgccgc attggcagcg atctcctttt tcggtctgca
    10501 acgagccatg cctgaaaccg ccacgcgtat aggcgagaaa ctgtcactga aagaactcgg
    10561 tcgtgactat aagctggtgc tgaagaacgg ccgctttgtg gcgggggcgc tggcgctggg
    10621 attcgttagt ctgccgttgc tggcgtggat cgcccagtcg ccgattatca tcattaccgg
    10681 cgagcagttg agcagctatg aatatggctt gctgcaagtg cctattttcg gggcgttaat
    10741 tgcgggtaac ttgctgttag cgcgtctgac ctcgcgccgc accgtacgtt cgctgattat
    10801 tatgggcggc tggccgatta tgattggtct attggtcgct gctgcggcaa cggttatctc
    10861 atcgcacgcg tatttatgga tgaccgccgg gttaagtatt tatgctttcg gcattggtct
    10921 ggcgaatgcg ggactggtgc gattaaccct gtttgccagc gatatgagta aaggtacggt
    10981 ttctgcggcg atgggaatgc tgcaaatgct gatctttacc gtcggtattg aaatcagcaa
    11041 acatgcctgg ctgaacgggg gcaacggact gtttaatctc ttcaaccttg tcaacggaat
    11101 tttgtggctg tcgctgatgg ttatcttttt aaaagataaa cagatgggaa attctcacga
    11161 agggtaaaaa aatgcctgat tgctttgtgc gatcaggcat tctcgaatta atggtgatgg
    11221 tcgtcaatct ggtgttcgat aaccatccct tcacctacgc tggcaagatg gcgaacataa
    11281 ggatgcgggc ggtaagccgg agctggcgca ggagccacat agacggtttg cggcacagtc
    11341 gcgacgctga ccgcttgtgg aacgctgact gaatcaggaa ccacaaccac tttgtatcca
    11401 ctcggcacat cgacggtgac actttgcgcc atcgccacac cagtaaagcc cattaaaagc
    11461 gcgcccaaca gtagacaatt tttcataaac atttcgcctg aaataatgca gcgggttaat
    11521 ttccgcgaat tatgcagcct tcattcaggt attgtgttgc cgggcgtgct gccgttttta
    11581 ttggtcaaat ggcgcttcgt gtttaagcac tttatcgatc acatccagta cgccttcacg
    11641 gttattggag cctgcccggt attttgccgc tgcgacgacc gcattgccgg cattttccat
    11701 tgcaaaacta aagcctgcct gacgcagcat ctcaatatcg ttaccgccat cgccaaagac
    11761 caccacttcg ctgtcgtcta ttccccataa tttctgcagt tggcgaaggc cattggcttt
    11821 atgtacgccg gggataatca gatcgatgct gccgttgccg gtgtggaccg gcaccataat
    11881 atcgccgatg gcctcatgta atgctttttg tacttgtgga atcagttcat cggaaagatt
    11941 caggccaaac ttaaagaaga tatcctctaa gttgtcaaag ttatcgacgt attccagacg
    12001 gtgataatac atttccgcca ccgttttcat ggcatcgtca tattttttga gcgtataggc
    12061 gctatttttt ccgcaggcaa taatttccac ttccgggcgc gtcagcaaat gttccacgac
    12121 agtagtaaac gcatccttcg acagctcgcc attaaaaaca tctttgcctt cgctcactac
    12181 ccagccgccg ttttcagcca caaaggcaat ttcattcgca atttcaggga agaaagagat
    12241 caactgataa tattgattcc cgctggcgac tacaaagcga attccttgtg ctttcatttg
    12301 ctgatactga gccataaacc gctcacggtt ataggttttt tgatcgctta agaaagtacc
    12361 atccatgtct accgcaatta atttaatgct catcaactat tctccatcgc cgtctgcgtt
    12421 ttggtatcgg gtttggcgac ggctttcgcg acaatagccg cgagaataac cagcgccagt
    12481 acaaccagca ttgcactacg taatccataa tgttcgccga gatagcccag cagcggcggc
    12541 ccgacgagga aagccagata accggtcgtt gctaccacac tgacgcgggt tggtgcatcg
    12601 gggccggtat cgctggcggc agaaatggtc agcgggaagc ccagcgaggc acccagtccc
    12661 cagagtacaa cagacacccc agcgacccag gcgctatcga caaaaataat cagcccaata
    12721 cccaacgccc ccattagtgc actggcccga accacggcaa cgcgactgta acggtcgatg
    12781 aaccaaccgc cggtgaagcg tccaacggtc atccccaggg taaaaccggc ataaatcagc
    12841 gagccggaag tagggctaaa accgtgaccg tcaaccatta ataagggtaa ccagtcgttg
    12901 gcagaacctt cggcaaaggc catcgccagc accacaacac ctatcagcag caactggata
    12961 tcgcgataaa aaggaatgcc tttttcgcca tgctgggtgc catcggcagc atttttgccc
    13021 gtaccgtcag ggattgcctg aatggcgata taaataggcg cgatacctac cagcgccgcc
    13081 aataaaatgt gcaccgttgc cggaacgcca aaggccgtca gtgccatccc gacaccagcg
    13141 cctgccagcg tgcccaggct ataaaaaccg tgcatcattg gcaaaaccgt tttattcatt
    13201 tctcgctcaa cggcggcacc ttcaacgttt atcgccactt ccgcagaacc aaaacttgcc
    13261 ccaaagacgc cgagaccaac ggcaaagagc aggggcgatg tcagccagag tgccagactt
    13321 aatatcatca tcccgatcaa tgcgcaggac atcgtgacca ggatgacatt acgcgtgcca
    13381 aagcgtttca ctaaccacgc cgagcagaga ataccgctca tcgaaccgat cgacagacca
    13441 aagagaacac cgcccatttc agcgatcgag acagagagaa tatcgcggat agcaggcgta
    13501 cgggttgccc aggacgccat taacaggcct ggcaaaaaga agaacataaa cagcgcccag
    13561 gttcggcgtt tcaatgcatt acgtgaagaa tttacggtca tagatcacgt caaaataaga
    13621 agagggaaga caacattagc aaggttgtgt acatttgtac acaattgcag agagaggaaa
    13681 tgacatgcgt cgcgctaacg atccgcaacg gcgagaaaaa attatccagg ccacgctgga
    13741 ggcggtgaaa ctttacggaa tacatgctgt tacgcaccgc aaaattgcta cccttgccgg
    13801 ggtaccgttg ggatcgatga cctactattt ttcaggaatt gatgagttgt tgctggaggc
    13861 gttcagcagt tttactgaga ttatgtcccg gcaatatcag gcatttttta gcgatgttag
    13921 tgatgctcaa ggcgcatgcc aggctatcac cgatatgatc tacagctcac aggttgcaac
    13981 gccggataat atggagctga tgtaccagct ctacgcgctg gctagccgaa aaccgctatt
    14041 aaaaacggta atgcaaaact ggatgcagcg cagtcagcaa acgctcgaac aatggtttga
    14101 acccggaacc gcccgcgcgc ttgatgcgtt tattgagggg atgacgctgc attttgtcac
    14161 cgaccgtaag ccgctatcgc gcgaggagat tttgaggatg gttgagaggg ttgcagggta
    14221 gtagataagt tttagataac aaaaaaccca tcaaccttga accgaaatgg cggggttgat
    14281 gggctccaca aaatggggac atcaaagaaa agcagtggca ctaattaaga ctgatgccct
    14341 gcggaaaagt tctgcggttg tgcaaaaaaa tttcattttc agggcaactt cagttttatc
    14401 ctaatcctgg ccataccatg acgatgattg tccctgccag cgtcagcagg acgttggcga
    14461 ttgcgtaggt gcccgcgtag cccagcgcag ggatgttact gcgagctgta tcactgatga
    14521 tctccattgc cggcgcgcag gtgcgtgcgc ccatcattgc gccgaacaac agtgcgcggt
    14581 tcattcgcaa tacataagca ccgaacaaga aacagataac cacgggcaca agactgacaa
    14641 ttaatccggc aatcaacatc tgaccgccaa tcgcgcccag gccgttatta ataccgctac
    14701 cggcgctcag accaacgcct gccataaaca ccatcaagcc gaactctttc accatgctta
    14761 atgcaccttg cggaatgtaa ccgaaggtcg ggtggttagc acgcataaag cccagcataa
    14821 ttccggcgaa taacaacccg gcagcgttcc ccatgccgaa actgaatgtg ctgaactgga
    14881 aggtgatcat cccgatcatc agcccaataa caaagaaggc gcaaaatgcc agcaggtcag
    14941 tgacctggct gtgaatcgag ataaagccga tgcgatcggc gatggttttt acgcgacggg
    15001 catcaccgct gacttgtaaa acgtcacctt tgttaagcac gacgttgtca tctatcggca
    15061 tctcaatctg gctacgaatg acgcggttaa ggaagcaacc gtgatcggtc aacttcagtt
    15121 gtgcgagacg tttacctaca gcgttatggt ttttaacgac cacttcttca gtgacgatac
    15181 gcatgtcgag aaggtcacga tcgaaaactt ctttaccgtt acggaagctg ggatcgagtc
    15241 gggcatgggc gtcgggatag cctaccaacg ctatttcatc gcccatttgt agcacggcat
    15301 caccgtctgg atttgccaga atcccgttac gtcgaatacg ttcaatgtag cagccggttt
    15361 gtcgataaat acccagttca cgcagatttt tgccgtcggt ccaggccacc agctccgggc
    15421 cgacgcgata ggcgcggatc accggtaaat aaaccttacg gttggcatca gtgtccaggc
    15481 cacgttcgcg ggcgatttgc tgggcgctgg tctgtaagtc ctgatgctgc aatttcggca
    15541 agtaacgcgc accaacaatc aaactcacca gaccgattaa ataggttaag gcatacccga
    15601 ggctcagatt atccagtgcc agtgagagct gcctgctttc catgccggaa tgacgcagtg
    15661 tatcgccagc accgaccaga accggtgtcg acgtcataga gcctgctaac ataccggccg
    15721 tcaggccaat atcccagcca aacagcttac ctaaccctaa ggcgatcacc agcgcactgc
    15781 caaccatcac cagtgctaac attaggtaat ttttcccatc gcgaaaaaaa atggaaaaaa
    15841 agttcggtcc ggcttcgacc ccgacgcaga aaataaacag cataaagcca agattaagcg
    15901 catcggtgtt aatgctgaaa tgttgttggc ctaataacag cgatacgact aaaacgccaa
    15961 tggaattacc cagttggatc gaaccaagtc gtaactttcc gagacatagc ccaagcgcga
    16021 ggaccacaaa taataacaga atgtaattcc catttaacaa ttcggcgacg tttatattca
    16081 cggaggctaa cttcttgttt actagtaagc tgttgaaaga aatggtaatt tacgataatg
    16141 ttttttacca gaattcaggg cgcagattca ttcagcgcac ctaaacgata gtaaagtaac
    16201 aatatatttt actagtgtaa tcacattagg tatcaacggc tatatgaatt gcgttggcct
    16261 atattagcat ggaatgcgaa gcggctttat cttactgaac gccacactgg cgaaaaatgt
    16321 gttcgataga cgcagtgtca ggaggaacga gtgaaacata aacaacgttg ggcgggggca
    16381 atctgctgtt ttgtcctctt cattgtggtg tgcctttttc tggcgacgca catgaaaggc
    16441 gcttttcggg ctgccgggca tcctgaaatc ggcttgctat ttttcattct tcctggagca
    16501 gtcgccagct tcttttcaca gcgtagagaa gtcctgaaac ctctgtttgg cgcaatgctg
    16561 gcggcaccct gttcgatgct cattatgcgg ctgttttttt caccgacgcg ctcattctgg
    16621 caagagctgg catggttact aagcgcggtg ttctggtgtg cgctgggggc actgtgtttc
    16681 ttatttatca gtagtttgtt taaaccacag cacagaaaaa atcagtaaag ccctcaacgc
    16741 gagggcttgt cagacgatca ggcgtccaga ttttctttca cccatgcagc aaaatcggta
    16801 tagccgccga tatgttgctg atcgacaaaa atctgcggca cggtttctac gggtttacct
    16861 gccttttgtt gtagatcttc tttagtgatc ccttccgcac gaatatctac atactgatac
    16921 tgaaaatcat cgcgttcatt gctcaatttc tcagccagat cttttgcacg cacacagtaa
    16981 gggcaacccg aacgaccaaa aataacggtt tgcattattt ctctcctcat agatttatgc
    17041 ctgtaatgat cacgctaaaa tgtattcgct gaaagtaggt ttaacctgtt gcattaattg
    17101 ctaaaagcta taactgttaa acacaataca gtgaaaagtt ttagactgaa ggctcacttt
    17161 gcagagggaa gcgtatgcgc gcgatcggta aattgcctaa aggcgtgttg atactggaat
    17221 ttatcggaat gatgctactg gcggtggcgc tgctgtcggt aagcgactcc ctgtcgctgc
    17281 ctgagccatt ttctcggcca gaagtgcaga ttctgatgat ttttctcggt gttttgctca
    17341 tgcttcccgc tgcggtggtg gttattcttc aggtggcaaa acgtcttgcc ccacagctga
    17401 tgaaccgtcc accgcaatat tcacgttcag aaagagaaaa agataatgac gccaaccatt
    17461 gaacttattt gtggccatcg ctccattcgc catttcactg atgaacccat ttccgaagcg
    17521 cagcgtgagg cgattattaa cagcgcccgt gcgacgtcca gttccagttt tttgcagtgc
    17581 agtagcatta ttcgcattac cgacaaagcg ttacgtgaag aactggtgac gctgaccggc
    17641 gggcaaaaac acgtagcgca agcggcggag ttctgggtgt tctgtgccga ctttaaccgc
    17701 catttacaga tctgtccgga tgctcagctc ggcctggcgg aacaactgtt gctcggtgtc
    17761 gttgatacgg caatgatggc gcagaatgca ttaatcgcag cggaatcgct gggattgggc
    17821 ggggtatata tcggcggcct gcgcaataat attgaagcgg tgacgaaact gcttaaatta
    17881 ccgcagcatg ttctgccgct gtttgggctg tgctttggct ggcctgcgga taatccggat
    17941 cttaagccgc gtttaccggc ctccattttg gtgcatgaaa acagctatca accgctggat
    18001 aaaggcgcac tggcgcagta tgacgagcaa ctggcggaat attacctcac ccgtggcagc
    18061 aataatcgcc gggatacctg gagcgatcat atccgccgaa caatcattaa agaaagccgc
    18121 ccatttattc tggattattt gcacaaacag ggttgggcga cgcgctaaaa ccgccacgtc
    18181 gatgtatgat acgcgggctt ttgaccaggt ctgacagaga ggtgcagggt gaaaattgcc
    18241 atattgtccc gggatggaac gctctattcg tgtaagcggc tgcgtgaagc cgctatacag
    18301 cgcggtcacc tggttgaaat tcttgatccg ctttcttgct acatgaacat aaatcctgcg
    18361 gcgtcttcta ttcactacaa aggccgcaag ttaccccatt ttgacgcagt gatcccgcgt
    18421 attggcaccg ccattacctt ttatgggacg gcggcactgc gccagttcga gatgctgggg
    18481 agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc gtgacaaatt gcgttccatg
    18541 caactgctgg cgcgtcaggg catcgacctg cctgtcacgg gcattgcgca ttcgccggat
    18601 gataccagcg atttaatcga catggtcggt ggtgcgccgc tggtggtcaa gttggttgaa
    18661 ggcacgcagg gaattggcgt cgtgctggcg gagacgcgtc aggcggcgga aagcgtgatt
    18721 gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg aatatatcaa agaggcgcaa
    18781 gggtgcgata tccgctgtct ggttgttggc gatgaagtgg tcgctgcgat tgaacggcgg
    18841 gcgaaagagg gcgattttcg ttccaatttg catcgtggcg gcgcggcaag cgtcgccagt
    18901 atcacaccac aggagcgtga aatcgcgata aaagccgcgc gaacgatggc gctggacgtt
    18961 gctggtgtgg atattctgcg tgctaatcgc gggccgttgg tgatggaggt gaatgcgtcg
    19021 ccggggctgg aaggaataga aaaaaccacc ggtatcgaca tcgcgggtaa aatgatccgc
    19081 tggatcgaac gccacgctac gacagaatat tgcctgaaaa cgggtggtta gtcgcaatca
    19141 cattactgat catggttttg cctgcgcttt ttgcgtaagc tgtgccggtc tttttatcga
    19201 aagaggttgt acaaaattat gacatcgctg gtcgttcctg gtctggatac gctgcgtcaa
    19261 tggctcgatg acctggggat gagttttttt gaatgtgata actgtcaggc tctgcatctg
    19321 ccccatatgc agaatttcga cggtgtcttt gatgccaaaa tcgatctgat cgataacacg
    19381 atcctgtttt ctgccatggc ggaagtccga ccttcagccg tattgccgct ggcggcggat
    19441 ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat ttcttgatat gcaggatgat
    19501 aatctgccaa agctggtggt ttgccagtct ttatccgtta tgcagggcgt aacctatgag
    19561 cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt cgatggtcat tcttgaagct
    19621 aatgcccatc aactgctgtt accgactgac gatgaagggc aaaacaacgt taccgaaaac
    19681 tattttctcc actgataatt cctttcgagc acgcagtcgc tgccgcagtg actgcgcgct
    19741 gcaaaattat ctgctgtttt taaccttttc ttaaagatta tttcacttat cttgtgtcga
    19801 tttggcttta tcacatagag caaatacgca taaaaatttg ttaaataccg ttttttaatc
    19861 cgagctatag tctcaaaccc tggctaaagt tattcttgcg atgcttttat atagcgagca
    19921 gtgctggccg ggagagagtt ctcttttctt acaccgcgcc gataaaaaaa tatgcacgtt
    19981 tattgcatat ctttcagtgt gacaactttt gttcgtttgt taacgaactt tcagaaggaa
    20041 agagatatga ccgccttaaa taaaaaatgg ctatcgggtc tggttgcggg tgctctgatg
    20101 gccgtctctg tcggcacgct cgcggctgaa caaaaaacac tccacattta taactggtct
    20161 gattatatcg cccctgacac ggtggccaat tttgaaaaag aaaccggtat taaagtcgtc
    20221 tacgatgttt tcgactctaa cgaagtactg gaaggcaaat taatggcggg tagcacctgc
    20281 tttgacttgg tggttccatc tgccagcttt cttgaacgcc agctgacagc gggagttttc
    20341 cagccgctgg acaaaagcaa attgccggag tggaagaatc tcgatccgga gctgctgaag
    20401 ttggtcgcca aacacgatcc cgacaataaa tttgctatgc cctatatgtg ggcgacgacc
    20461 gggattggct ataacgttga taaagttaaa gctgtgctgg gcgaaaacgc gcccgtcgat
    20521 agctgggact tgatcctcaa gcctgaaaat ctggaaaaac tgaaaagctg cggtgtctct
    20581 ttcctggatg cgccagaaga agtttttgct accgtgctga attatctcgg caaagatccc
    20641 aacagcacta aagcggatga ttacaccgga ccggctaccg atctgctgtt aaagctgcgc
    20701 ccgaacattc gttatttcca ttcatctcaa tacattaacg acctggcaaa cggcgatatc
    20761 tgcgtcgcta ttggctgggc gggcgatgtc tggcaggcgt caaaccgcgc gaaggaagcg
    20821 aagaatggcg ttaatgtctc gttcttgatt ccaaaagaag gggcgatggc gttctttgat
    20881 gtattcgcca tgcctgcgga tgccaaaaac aaagacgaag cctatcagtt cctgaattac
    20941 ctgctgcgcc cggatgtagt agcgcatatt tccgaccatg tgttctatgc caacgccaat
    21001 aaagcagcca cgccgctggt gagtgcggaa gtccgtgaga acccgggtat ttatccgcct
    21061 gcggatgttc gtgcgaagct gttcactctg aaagtgcagg acccgaaaat cgaccgtgtg
    21121 cgcacccgcg cgtggaccaa agtgaagagc ggaaaataat ccgcagcctt agatgccggg
    21181 cgggcgcacc acacccgccg gcaattcgca ccatcatggt gcgcttgcac acattcaatg
    21241 ccggagagca gccgtgaatg acgctatccc tcgcccgcag gcgaaaaccc gtaaggcgct
    21301 gacgccgcta ttagaaatcc gcaacctcac caaatcctac gatggtcaac atgcggtgga
    21361 tgatgtcagc ctgaccatct acaaaggtga aatcttcgcg ctgctgggcg catccggctg
    21421 tggcaagtcc acgctgctgc gtatgctggc aggtttcgaa caaccttctg ccggacagat
    21481 aatgcttgat ggcgtcgatt tgtcacaggt gccgccttac ctgcgcccca tcaatatgat
    21541 gtttcagtct tacgcgctgt ttccgcatat gaccgtggaa cagaacatcg cttttggcct
    21601 gaaacaggac aaactaccga aagcggaaat tgccagtcgg gtccatgaga tgctcggcct
    21661 ggtgcacatg caggagttcg ccaaacgcaa accgcatcag ctttccggtg gtcagcgaca
    21721 gcgtgtggcc ctggcccgaa gccttgccaa acgcccgaaa ctattactgc tcgatgagcc
    21781 gatgggcgcg ctggataaaa agctgcgtga ccggatgcag cttgaagtgg tggatattct
    21841 ggagcgcgtc ggtgtgactt gtgtgatggt cacccacgat caggaagagg cgatgaccat
    21901 ggcggggcgt atcgccatta tgaatcgcgg gaaatttgtc cagattggcg agccggaaga
    21961 gatctacgag catccaacta cccgctacag cgctgaattt atcggttcgg ttaatgtctt
    22021 tgaaggtgta ctcaaagagc gtcaggaaga tggcctggtg cttgattcgc cggggctggt
    22081 gcatccactg aaagtcgatg cggatgcttc agtggtcgat aacgtgccgg tacacgtagc
    22141 gctgcgcccg gaaaaaatca tgctttgcga agagccgccc gccaatggtt gtaacttcgc
    22201 ggtgggggag gtgatacaca ttgcctatct cggcgatctt tcggtgtatc acgttcgtct
    22261 gaaaagtggg cagatgatta gcgcccagct acaaaacgcc catcgtcatc gtaaagggtt
    22321 accgacctgg ggcgacgaag tgcgtttgtg ctgggaagtg gacagctgtg tggtgctgac
    22381 ggtttaagga gcaaagatga atacacttga acctgctgcc cagtcgaaac cgccgggcgg
    22441 atttaagctg tggttgtcgc agctgcaaat gaagcatggg cgcaaactgg tcattgcgtt
    22501 gccatatatc tggttgatct tgctgtttct gctgccattt ctgattgtct ttaaaataag
    22561 cctggcggag atggcgcgcg ctattccacc ttataccgaa ctgatggagt gggctgacgg
    22621 gcaactgtcc atcactctta atctcggtaa ttttctgcaa ctgaccgacg atccgctcta
    22681 tttcgatgct tatctccagt cgctacaggt ggctgcgatt tcgacatttt gctgtttact
    22741 gatcggctat ccgctggcgt gggcggtggc gcacagtaag ccttcgaccc gtaatatttt
    22801 attactactg gtgatcctgc cgtcgtggac ctcgtttctg atccgcgttt atgcctggat
    22861 gggaatatta aaaaacaacg gtgtgctgaa taattttctg ctgtggctgg gggttatcga
    22921 tcaaccgctg accattcttc ataccaatct ggcagtttat attggcattg tttacgccta
    22981 cgtgccgttt atggtactgc cgatttatac cgcgttgatt cgtattgatt attcgctggt
    23041 ggaagcagcg ctggatctcg gtgcacgacc gctgaaaacg ttctttactg tgatcgtgcc
    23101 gctgactaaa ggtgggatta ttgccggatc gatgctggtg tttatcccgg ctgtgggcga
    23161 gtttgtgatc ccggaactgc tcggtggccc ggacagcatc atgatcgggc gcgtgctatg
    23221 gcaagagttt ttcaataacc gcgactggcc ggtggcctcg gcggtagcga tcatcatgtt
    23281 gctgctgcta attgtgccga taatgtggtt ccacaaacac cagcaaaaaa gcgtgggaga
    23341 acacggatga ataatttacc ggtagttcgt tcgccctggc ggattgtgat tttgctgctg
    23401 ggctttacct ttctctacgc gccaatgctg atgctggtta tctattcgtt taacagctcg
    23461 aagctggtga cggtgtgggc cggctggtca acgcgctggt atggtgagtt attgcgcgat
    23521 gacgcgatga tgagtgcggt tggtttaagc ctgacaattg ctgcctgtgc ggcaacggcg
    23581 gcggcgatcc tcgggactat tgcggcggtg gtgctggtgc gctttggcag atttcgcgga
    23641 tcaaatggct ttgcctttat gatcaccgcg ccgctggtga tgccagatgt catcacgggc
    23701 ttgtcgctgt tgttattatt cgtcgcgctt gctcatgcca ttggctggcc tgcggaccgc
    23761 ggtatgctca ccatctggct ggcgcatgtc actttttgta cggcttatgt ggcggtcgtt
    23821 atttcttcaa gattgcggga actggatcgc tcgatagaag aagcagcgat ggatctcggt
    23881 gcgacgccgc tgaaagtgtt tttcgtcatt acgctaccga tgatcatgcc cgcgatcatt
    23941 tctggctggt tactggcttt tactttgtcg cttgatgatc tggtgatcgc cagctttgtt
    24001 tccgggccgg gagccaccac gttaccgatg ctggtctttt ccagcgtgcg gatgggggtg
    24061 aatccggaaa tcaacgccct tgcaacgtta attctcggtg cggtcggaat tgtcggattt
    24121 atcgcctggt atctgatggc tcgcgcagaa aaacagcgga tacgcgatat ccagcgtgca
    24181 agacatggct gaagacacta aaatttgcca acctggctac ataatgccgc gcatgccgcg
    24241 gcattgtttt catggaagac gaaacgttgg gattttttaa gaaaacatct tcatctcatg
    24301 ctcgcctgaa tgtgcctgcg ctggtgcagg tggcggcgct cgccattatt atgatccgtg
    24361 gcctcgacgt gctgatgatt ttcaatacgc tgggcgtgcg cggtattggc gagttcattc
    24421 atcgcagcgt acaaacctgg agtttaacgc tggtcttttt aagcagtctg gtgctggttt
    24481 tcattgagat ctggtgtgcg ttttcactgg tgaaagggcg tcgctgggcg cgctggctat
    24541 atctgctgac acaaatcacc gccgcaagtt acttgtgggc ggcttcgctg gggtacggtt
    24601 atccggagct gttcagcatt cccggtgaat caaaacgtga aatcttccat agcctgatgc
    24661 tgcagaagct gccggatatg ctcatcctga tgctgctgtt cgttccctcg accagtcggc
    24721 ggttcttcca gttgcaataa tgtgtataat cgtcgcccct gatgatgtga aggtcaatgt
    24781 atgcagtgcg cactttacga cgcgggtcgc tgtcgttcct gtcagtggat aacgcagccg
    24841 attccagagc aactctccgc taaaaccgcc gatcttaaaa atctgctcgc cgattttccg
    24901 gttgaggaat ggtgcgcgcc ggtgtcaggc ccggaacaag ggtttcgtaa taaagccaaa
    24961 atggtggtga gtggtagcgt tgaaaaacca ctgctcggta tgctgcatcg cgacggcaca
    25021 ccagaagacc tttgtgactg cccgctttat cctgcctcat ttgcgcccgt ttttgcggcg
    25081 ctaaaaccgt ttatcgcccg tgcggggctg acaccctaca acgtggcgcg taagcgtggc
    25141 gaactgaaat acattctgct gactgaaagc cagagcgatg gcggcatgat gctgcgcttt
    25201 gtactgcgtt ctgaaaccaa actggcacaa ctgcgtaagg cgctgccgtg gttacaggaa
    25261 caactaccgc aactgaaagt tattaccgtc aatattcagc cggtacatat ggcgattatg
    25321 gaaggggaga cggagatcta ccttactgaa caacaggcgc tggcagagcg ttttaatgac
    25381 gtaccgctgt ggatccgtcc gcaaagtttc ttccagacta atccggcggt cgccagccag
    25441 ctgtacgcta ccgcgcgcga ctgggtacgg cagctgccag ttaaacatat gtgggatctg
    25501 ttctgcggtg tgggtggctt tggtttacac tgcgcgacgc ctgacatgca gttaaccggg
    25561 atcgaaattg catcagaggc cattgcctgt gcaaagcagt cagccgctga actgggctta
    25621 acgcgtttgc aatttcaggc gctggactcc actcagtttg ccaccgctca gggggaggtg
    25681 ccggagctgg tgctggttaa tccgccgcgc cgcggcattg gtaaaccgct atgtgattat
    25741 ctctcaacaa tggcaccgcg ttttattatc tactccagct gtaacgccca aaccatggcg
    25801 aaagatgtcc gcgaactgcc aggttaccgc atcgaacggg tacagctttt tgatatgttc
    25861 ccgcacaccg cgcactatga agtgctgacg ctgctggtga agcaataaaa aagccgcagg
    25921 tgcggcttca gattgctgat caggtgcgct ttgttcatgc cggatgcggc gtgtacgcct
    25981 tatccggcct acaaaatcat gctaaatcaa tacattgcag agttgatgtg ggccggataa
    26041 gcgcagcgca tcaggcaatc ttacttttgt catcagtctc aattactgcg gaaaccactg
    26101 gtcattgatt ttctgatatg taccgtcagc tttaattgct gccagcgcgt tattcagttt
    26161 ttccagcaga gctttgttat ccggacgtac agcgatgccc agaccggtgc cgaagtattg
    26221 cggatcggtc actttttcag tagcaacgcc cagttgcgga ttggttttca gccattcgtt
    26281 taccaccgct gtgtcaccaa ataccccatc aatacgacca tttttcagat cgataaaggc
    26341 attctgataa ctgtcatagg agacagtttt cacttcgggg tgctgatcct gaatatattt
    26401 ctggtgcgtg gtgccgtttt ccatcccaat acgtttgcct ttcagatcgg caaacgtttt
    26461 gtaggtatct tttttggcaa tcacgacggc tgagttttca tagtagggcg tggtaaacga
    26521 tacctgcttg ctacgctcgg gcgtgatatc cataccggag attacggcgt catattttct
    26581 gaatttcagt gacgggatca ggctgtcgaa cgcgtgatta gtaaaagtac attctgcctg
    26641 catttgtttg cacaaggctt ttgccagatc gatatcaaag ccgacaatct cattattagc
    26701 acctatagat tcaaagggtg gataggtggc tgaaacgcca aaattgattt tctctgcggc
    26761 agaagcaccg aaagtaaagg aagcaagtaa agcggcaaga actaactttt tcatgatgga
    26821 actcccgtct gtcaatctta tgatttttgg ccgtgtctgc ggcatgggat aacaatgcca
    26881 tcaagtgaat ttatatgcaa taaatatgat taaataattt aaatgaaata aaaaaagacg
    26941 gataccttag tgagttgtcc gtcttcatta taagaattta tgcactatgt aggccggata
    27001 aggcgtccac gccgcatccg gcacaggcac cgtgctgatg tctgatgcga cgctggcgcg
    27061 tcttatcaga cctacaaacc cccccggcga atgtacgcag ctacattaat ttcgccgttc
    27121 gaatgccagt gctttgcgct cgatcagacg catcatcagc gtcagcaggc cgttgacgac
    27181 caggtaaata atccctgccg caccgaacac cattacatcg taggtgcgtc cgtacaacaa
    27241 ctggctgtat cccatcactt ccatcagcgt aatggtgtaa gccagagagg tacttttgaa
    27301 caccagcacc acttcgttgg aataagaaga gagcgagcgt ttaaaggcat acggcagcag
    27361 gatcgccagc gtatcttttt tgctcattcc cagggcgcta caggactgcc actgaccttc
    27421 tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta tacgccgcac tgttcagcga
    27481 taacgcaatc agcgcacata accacggttc tgacaacaaa tgccacagtg ccggatactc
    27541 ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg aagatctgca ccagcagcgg
    27601 cgtaccggta aacagcgtga tataaccccg caccagccac accagcaccg gcgttttcag
    27661 cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc acaatcagcg aggcaacggt
    27721 tagcgtcagg ctggtgtgta gccctttcat cagttcgggt aaatactcaa acattagctg
    27781 ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt tgagaatgta ctgactgagc
    27841 agggtgatca ccaggtaaat cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta
    27901 cgagtagcga tgctttttgt ttgcagcatt aaatcattca cactaatcaa actgaccagc
    27961 gcggtatctt tcagcagcac cagccactgg ttaccgaggc caggcagcgc atgacgccac
    28021 atctgcggca tcaccagacg gaaaaagata gccgatttcg acagccccag cgcctgacca
    28081 gactcccact gacccaccgg caccgctttc aacgcgcccc gaagcgtttg cgaggcatag
    28141 gcggcataca gcagtgacag agcgatgaca ccacaaagga acgggctaac gtcgaagttc
    28201 tcaatgtcca tctgcactgg gatctgcacg aacccaagat tgatagtgaa gccatccgaa
    28261 agcgtcagca gcagctgcga ggagccaaaa tagataaaca gcaccaccag aatttctggc
    28321 aggccacgca gaatggttac cagcgctgaa cctgcccacg cgacaggacg ccattttgcc
    28381 gactcccata ccgcaaagaa catcgccagc gccagcccga caatcaatgc acaaacggca
    28441 aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa attcattcat caggaattac
    28501 ttctggaacc atttgttgta gatggtttcg taagtgccat ctttcttcac tttttccagc
    28561 gcagtgttga gtttctgctg cagctcagtg ttgccctgac gtaccgcgat gccgaggcca
    28621 gtgccgaagt aatctttatc ggtcactttg tcgcccaccg ccgccagttt cgggttatct
    28681 ttcagccatt cggttaccac tgcggtgtca ccgaagacgc cgtcgatacg cccgttttgc
    28741 agatccagtt ttgcgttctg gtagctgtca tacggaacgg tagtgatttc cgggtgctta
    28801 tccataatga atttctggtg tgtcgtcccg ttctgtacgc cgactttttt gcctttcagc
    28861 tgatcaacac tggtgtattt gccttgctga cccacaaaca gggcagagtt gtcatagtac
    28921 ggggtggtaa acagcacctg cttttcacgc tccggagtga tatccatgcc cgccatcacg
    28981 gcttctacgc gacggaattt caggcttggg atcaggctgt caaacgcctg gttagagaaa
    29041 gtgcaggttg catcaatctc tttacacagc gcttgtgcca ggtcgacgtc aaaaccaacg
    29101 atctggttgt ttgcatcaat cgattcaaac ggaggatagg atgcttcggt agcaaaacga
    29161 atggtttcgg cagctgtggc ggaaagacta aaacctgcaa ttaacgcggc aatcagaact
    29221 tttttcattg ttgttatccc gaatcttagt gagagagata gtttttaaat gcttcggttt
    29281 gcggctcggt aaagcagctc gcgtcgcctt gttctacgat atgaccattt tccatataca
    29341 ccactcggct ggcggtttta cgcgccactt caacttcgtg ggtgacgatc acctgggtaa
    29401 tattcgtttc tgccagctca cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca
    29461 gtgcggcggt cggttcatcg aacagcagta cctgcggttc catcatcaac gcacgggcaa
    29521 tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt
    29581 tgagacgcag acgttccagc agtttttctg cacgggccag cgcctgatct ttactcaacc
    29641 ccagtacacg gcagggcgct tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt
    29701 tgtattgctg aaacaccatg ccaacgttac gacgcaaatc gcgaatcgct ttgtcagagg
    29761 gtgttttggt aaaatcgaaa tggttgcctg caatgttgag cgtaccggag cgcggcatct
    29821 caagcagatt gagtacacgc agcagcgagc ttttacccgc gccgctgggg ccaagtaaca
    29881 ccagcgtttc gccctgtggg caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt
    29941 agaagcaatt aatgccgttt aattgaatac tcattgacac tcgtatactg gcagtctgat
    30001 agctattgag gtcgaagata gtacctttga cagaataatt atgcaatatt tctgctttaa
    30061 aagttaaaag caaagcgcat tattcaataa acatagcaca aaataacggg ggcggtggtc
    30121 ggtgagcata aatgtcggca ttcctcacga aatgccggac aatttacggg gtttattggt
    30181 tgatcaaggc gttagcgatt ctcgatggac tgacggagcg tacccgccgt ggcatgaacg
    30241 ctgccgccta aatagcgtac atcgtcgatc acccaacact ggccttcctg aatcattaac
    30301 acttcatcct gccagctctg atcaccctgt tttagatcca cgcgcaatgg aatgttacgg
    30361 gcatcacgat tagggatagt cgatgcactg gcaacgtggg cgctgtctgg caaggtggtt
    30421 cgactggaga atggatcgtt ggtcagtagt tcccgatggt tattatcccg ggaggcatcg
    30481 ctaagcagtg tcgccagttt gtcgctcaga taagggcgca aggcggtgat gtcgttgctg
    30541 cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt tatccgggcc tccttcaacg
    30601 caaggaccac tgcgtgtgcc gttatcttta taagctggag tgactgtggt gcaggcactg
    30661 aggagcagtg cgcaggggat aagcattgtc aatttgctgt agcgcataat gatttcctta
    30721 taagcgatcg ctctgaaagc gttctacgat aataatgata tcctttcaat aatagcgtat
    30781 cagtccgata atgcttttga gatcgaaggc ttaacaaaca aggagatcga tcatgcaatt
    30841 ttctacaacc ccaactctgg aaggccagac catcgttgaa tattgcggtg tggtgaccgg
    30901 cgaagcgatt ttaggtgcca atattttccg tgatttcttt gctggtatcc gcgatatcgt
    30961 tggcggacgc tccggtgcgt atgaaaaaga actgcgcaaa gcgcgggaga tcgcctttga
    31021 ggaacttggc tcccaggccc gggcgctggg ggccgatgcc gtcgtcggta ttgatatcga
    31081 ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt agcgtcagcg gtacggcggt
    31141 gaaaacgcgt cgatgagaag agtcttctgg ctgatcgctg ccgctctgtt actggcaggg
    31201 tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc agcttgatac ccgacgccag
    31261 gcgcaggcgg cgtatccgcg cattagagtg ctggtgatcc actacaccgc agatgatttt
    31321 gatagctcgc tggcgacact gaccgataag caggtcagct cgcattatct ggtccctgcg
    31381 gtaccaccgc gatacaacgg taaaccgcgc atctggcaac tggtgccgga acaagaactg
    31441 gcctggcatg cggggattag cgcctggcgc ggggcaacgc gccttaacga cacctctatt
    31501 ggcattgagc tggaaaaccg tggctggcaa aaatcggccg gagtgaaata ttttgccccg
    31561 tttgaaccgg cacagattca ggcgcttatt ccactggcga aagatattat tgcccgttat
    31621 cacatcaagc cggaaaacgt agtggcacat gcggatatcg caccgcagcg caaagacgat
    31681 ccggggccat tatttccctg gcagcaactg gcgcagcagg ggattggtgc ctggccggat
    31741 gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc acacaccggt agataccgcg
    31801 tcattgctgg agcttttggc gcgctacggt tatgacgtta aacctgatat gacaccgcgc
    31861 gagcagcggc gcgtgattat ggcattccag atgcatttcc gcccgacgtt atataacggc
    31921 gaagcggatg cagaaactca ggcgattgcc gaagcattgc tggagaaata cgggcaggat
    31981 tagcgcggca gttttccgtg gtcgcgtagc caggcggcag ttttctcgat accttcatcc
    32041 agggtgatga ccggctgata acctaactct tcctgcgcac gcgtaatatc cagcgtaaag
    32101 tcaaaattca acttggagac gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg
    32161 cctaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaggggac ggaacgaata
    32221 cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc
    32281 tcgccgttgg tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt
    32341 gccagccaca ttgcgtgcac ggcattttca tagtaggtca tatcgaccag cgcactgccg
    32401 ccatgcggta acagaatact gccgtagtgg tgcatcatat gcgccagacg gggaataaag
    32461 actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga
    32521 ttcgcctgcg aaagcatatt gatcacttct tcgctggctg ctttgctgcg ggcaaactcg
    32581 ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag
    32641 tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac agcccattca
    32701 cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccagggt
    32761 gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt
    32821 gatgaaacca gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat
    32881 ttgcccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac
    32941 tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc
    33001 cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat ttcccccatg atgcaatggg
    33061 aaacatgaaa gaataacgca ggttttgtcg attaatctgt gctttgttct gccagtctgg
    33121 cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt
    33181 aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt
    33241 cgcctttatc ttccaggcta aaacacagtc gtcccagccc cggcgctttc atgccaaata
    33301 acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatct accgcatcgc
    33361 cagtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg atcgcgcggt
    33421 ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga
    33481 tttggttcac tacctgccat aaagcagcaa ggctggcgga cgttttaacg gtaaaccccg
    33541 cctgtttggc aaaataaccg tactccggtc gccagcgggc aaaggcctga gcgtcgtagc
    33601 cccagtcgct ggagttgacc agtttttcct cctctttcaa cgtgctacgt accgcgtcat
    33661 cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc ggtatcatcc gccagcagat
    33721 cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat
    33781 tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct
    33841 taccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg
    33901 cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa caacgccacc agatagtgca
    33961 gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc ggcgttaaca
    34021 ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc cgctgaacct gcgccaacga
    34081 taattccggc ccgaagttcg gtcacaggta cattcgcttc acgaagaatg tccgccgtag
    34141 cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac gaactgagaa
    34201 agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga gccacctggc
    34261 gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg caccagaaaa tagacggtat
    34321 cgatatcctg caacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac
    34381 tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct
    34441 gatgcccttg ctggctgagt gtgcgcacca gatgctgacc aatgtagcca ctggcaccga
    34501 gaactaaaat gcgttgcggc acgtctctct ccttaacgcg ccaggaatgc gcgccagtgg
    34561 gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat gcgtcaccag gcggacaatc
    34621 ggcgaggcgt taatcagcac gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt
    34681 tcttccccga cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc cgcgcctgct
    34741 tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa ccgtgcgacg
    34801 ttatttttca gggcatacat cccggcggca gccagaatcc cggactgacg catcccgcca
    34861 ccggccattt tccgccagcg gatggcacgt ttaatgtaat cacgattacc gacgagtaat
    34921 gaaccgactg gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc
    34981 gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg cgcaccgtca
    35041 acatgcagcg ccagattgcg ctcgcgggta aattcccatg cttctttcag gtattcacgc
    35101 ggcagcactt tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg
    35161 atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc
    35221 gcgtcgatgg gttgcggctg aatactgccc agcaccgctg cgccaccggc ttcaaacaga
    35281 tagttatgcg cggcctgacc gacaatatac tcttcgccac gttcgcagtg actgagcaga
    35341 gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc
    35401 tctgctgtat agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca
    35461 accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg
    35521 cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc
    35581 cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgataat tgcgcgcaac
    35641 atatacaggc taaaaccttt ggcctgttcg agtttgatct gcggtggaat ggctaactct
    35701 tctttggcga ccaccacatc caccaacacc ggaccgtcga tagagaaggc gcgttgcagg
    35761 gcttcatcaa cttcagacgc tttttctaca cggatacccg taatgccgca cgcttcggca
    35821 atgcgggcaa agtttgtgtc gtgtagttcg gtgccgtcag tcaaatagcc accagctttc
    35881 atctccatcg ccacaaagcc cagcacgctg ttgttaaaga cgacaatttt cactggcagt
    35941 ttcatctgca ctactgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac
    36001 atggcgacca cctggcgttc cggctcggtt gcctgcgcac ccagcgcctg cggcatggcg
    36061 ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt cattttcaga
    36121 taacgtgccg cccataccgt tggcgtgccg acgtcacagg taaagatggc gtcatcggcg
    36181 gcaaaatgac taatttgttg cgccagatat tgcgggtgaa tggctttctc gctcggttta
    36241 gctaaatcgt ccagcccttt gcgggcgtcg cggtaatctt ccagcgcttt atccagaaac
    36301 ttgcgatcgg ttttttcttc caccagtggc agcaatgccc gcagggttga tttgatatcg
    36361 ccgaccagcg ccatatccac cttgctgtga gcgccgatgc tggctgggtt gatatcaatc
    36421 tgaatgattt tagcatcggt cgggtagaag gcgcggtagg gaaattgcgt gccgagcagc
    36481 actaacgtat cggcattcat catggtatgg aaacctgacg agaagccgat taatcccgtc
    36541 attccgacat catacggatt atcgtattcg gcatgctctt taccgcgcag ggcatgaaca
    36601 ataggcgctt taattttccc ggcaaactca actaactctt tatgcgctcc cgcgcagccg
    36661 ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac
    36721 tcttcttctt ccggcgtcac gactggctgt ggcgcatgat accagtgggt agttgcccct
    36781 tctggcgcag gttttaacgc cacgtcgcct ggtaacacga caaccgaaac gccacggtta
    36841 agcaccgctt tgcgcatggc aatcgccagt acttgtggga tctgctccgg gctggaaacc
    36901 agctcgcaat agtggctaca ttcgcggaat aactcttgcg ggtgggtttc ctggaaatag
    36961 ccgctgccaa tttcgctgga gggaatatga gcggcaatag ccagtaccgg aacgtgattg
    37021 cggtggcaat cgaagaggcc gttgattaag tgcaggttgc cggggccgca cgatccggca
    37081 cagaccgcca gttctcctga aagttgtgct tcagcgccag cggcaaacgc cgccacttct
    37141 tcatggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc actaagaccg
    37201 ttcagagagt cgcctgtgac tccccagatg cgtttcacgc ctgccgattc gagtgttttg
    37261 gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta
    37321 acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt
    37381 taaatagccc gatgacagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc
    37441 ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc
    37501 gtcattgtgc tgctcaccgt atattcaccg gaaaccactt tcgtcttaca gcagccgcaa
    37561 acacccgcac ggcaggcagc gacaaccgga acgttattgc tttccaacgc ctccagtagc
    37621 gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa tttcagaccg
    37681 ctggtcgccg cttccgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg
    37741 agcgctttca cttcctgctc tacccaatcc atatacggag ccgggccgca ggtcatcacg
    37801 gtacgtgaag ctaagtcagg aacgcgagtc agcagttcgc gagtgagacg accagcgata
    37861 aagccttcgg taacgttatt ttccgccacc agcgttaccg gatagttacg ccactcatcg
    37921 gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cctgtacatc ggcctgtgga
    37981 cgattcttcg ccagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgccgcc
    38041 agcagcagga atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa
    38101 agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc gacaccgtca
    38161 tcaatccgcc gcacggtcag ggtgatgtat tcactcacgc ctggcgtgga ggaaatggtg
    38221 taagcacgca gcgtttccgc tgagtttcgt acgctgacca gcgcatattg cccggcgcga
    38281 tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgcgta
    38341 atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat cgacaaactc
    38401 cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga
    38461 atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg
    38521 tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc gctttctgtt
    38581 caaaccagga gagcaccagc gacagcggca gatcgttgac accgcagccc agtttctctg
    38641 ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct gcatctacca
    38701 gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg
    38761 cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg aagtagtggc
    38821 gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc
    38881 tcaccagatc aatcagcgta tccgctgcgc caagcagcgt ctggcggcca aagcccacgg
    38941 tgatcaggtg cggaatttcg ctgtacggga agcccgccat ttgttgcgcc tgggcgatga
    39001 ccgcagaaaa atcttcgcca tccagatgac gcacacccgg ccagccaacg atgctgcggg
    39061 tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca
    39121 cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg ctgccgtagt
    39181 taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc
    39241 cgtgggtgta aacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt
    39301 tgagatcgtg accggaaatc aggatgcatt tacccgccgt cgctttgacg ttgacctggg
    39361 ttggcgtcgg gtgtccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga
    39421 agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg
    39481 tccccagcca cgccatgatt ttatgatact gggcataaat atcgttgtcg tattgaccga
    39541 gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac
    39601 gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg
    39661 cctgacgttg cagctcgccg ggatcgtcgc tcaccagttg caggtcagcc atcgggttat
    39721 cgacgcgcgc gttggcatct acagccaggc attgtgcttt cagcgcctcg cgcaaggcaa
    39781 tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg gtcagggttg
    39841 agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt gatgatgccg tattcacgcg
    39901 cttttaccgc ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag
    39961 aagtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag
    40021 tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta
    40081 atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt tcatgcaact
    40141 ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag agaggtatta
    40201 cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat
    40261 tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg tgaaatccat
    40321 tgatgtggca ccgcataagc ccagtgcagt agagcggctg cggcgaatca gcccagggat
    40381 caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc tcccgattac
    40441 cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga aaccggaggc
    40501 cattgccagc gcggtattga tggggagatc aagaataaag gcgttaatta aaccaccaat
    40561 taatgaactg acaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg
    40621 ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta gcaaaattaa
    40681 cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca gaccaatggc
    40741 aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg caatacgcga
    40801 cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca gccacatcag
    40861 ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc
    40921 caacaggtta ctggcgaggt tatcgagaaa cgccagactt atacccataa aaaagagaat
    40981 aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt gttggcgaag
    41041 cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag aaaacatgaa
    41101 aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg aaagcggaag
    41161 gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca gagggggatt tttaatccta
    41221 tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa gccactggcg
    41281 cagggagcgc gcagggggcg gccagtcgcc gccgccccct gcacccccgg gctctggcga
    41341 acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggctaccgg gtcgggcgcg
    41401 aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc ccggccttcg
    41461 gggaacgctt cagcgatttt gacgccacca acacccgtgc ggtttgtaac gtcgaagata
    41521 aaacaaccag ggcagataaa atgccaatgc taattaatta cgtttttcca gcagggtccg
    41581 gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga accacagttg
    41641 ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg ggttaacaga
    41701 agtgttagtc accggaatac taattaagtg aatcagggtt aaggccagac caatagcgat
    41761 cggcgcaaaa cctgccggcg cgaatttgtc ggttgcgccg tggatcacca acaggaaacc
    41821 tgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc ctggtgaatg
    41881 ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg ttttaccact
    41941 ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg caattacgta
    42001 gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag tgaccgccgg
    42061 gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc
    42121 gaacgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg cggccagtac
    42181 agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag ctgctaattt
    42241 tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc gttttatagg
    42301 gtcgtcgtaa aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt
    42361 cattcaccag ataaataaat ccagtaaatt tgatttaggg caacagcggg ttgccccata
    42421 tagtcatttg tctgattgac agtgtagtgc aagcaaaaga tttaatcctt taggcgtaat
    42481 aaaaaataat ttatcatgct aattatttga ttttgttgtt tttgcagact tatcagcaag
    42541 agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt aaatgtgatg
    42601 tcatcacgta ttagcaaggc ctttcccgtt atactgccag cgtaaaggat aagtcacata
    42661 tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc
    42721 gtttgtcgtt gatgctcgaa caaaacaacg tcctgattgg ggagaacgcg tggggtaaat
    42781 ccagcttgct ggacgcctta accctgctgc tatcgccaga atcagatctc taccattttg
    42841 agcgcgacga tttctggttc ccgccgggag atatcaacgg gcgagaacat catctgcata
    42901 ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggttcgccgt tatcggccgc
    42961 tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg tattttttat cgtctggaag
    43021 gggagagtgc ggaagacggc agcgtgatga cactgcgcag ttttctcgat aaagacggac
    43081 atccgattga tgtcgaggat attaacgatc aggcacgcca tctggtgcgt ttaatgccgg
    43141 tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg gtgccaaatg
    43201 ttcccaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag ttgtcctcac
    43261 atccgcaaaa tctctctgat ggacagattc gtcagggact ttccgcaatg gtgcagttgc
    43321 ttgagcatta tttctctgag cagggggccg gacaggcgcg atatcgttta atgcggcggc
    43381 gggccagcaa tgagcaacga agctggcgct atctggacat catcaaccgg atgattgacc
    43441 gacctggagg gcgctcgtat cgggttattt tgctcggcct gtttgctact ttattgcagg
    43501 caaaaggcac attgagactg gataaagacg cgcgtccatt gttgctgatc gaagatccag
    43561 aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat cttctgccat
    43621 tgcagcgcat tgccaccacc aactcgggtg agttgctttc gttaacgccg gtagagcatg
    43681 tttgccgact ggtacgtgag tcctcgcgcg ttgccgcctg gcgtctgggg ccgagtggct
    43741 tgagtaccga agatagccga cgcatatcct ttcacattcg ttttaaccgt ccgtcatcgc
    43801 tgtttgctcg ctgctggttg ctggtggaag gggaaacgga aacctgggtt atcaatgaac
    43861 tggcgcgtca gtgcggacat cattttgatg ccgaagggat caaggtcatt gagtttgccc
    43921 agtccgggct aaagccactg gttaaatttg cccgccgaat ggggattgaa tggcatgtac
    43981 tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc ctgttgagta
    44041 acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat atggaacatt
    44101 ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg
    44161 taccgatgaa tctgcgcaaa attatctcga aagcgatcca tcgctcttcc aaacccgatc
    44221 ttgccattga agtggcaatg gaggccggac gtcgtggtgt ggactccgta ccgacgctgc
    44281 tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa
    44341 acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga cggtattcag
    44401 cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgct ggaaggcggt
    44461 aatggcgttc agcatcacat acgccgccaa ccgattccca gaaagcgttg taatcggcat
    44521 ggatcttgcc ttctttatca cgataacgca ggctgcggta aatatgcgtt tcattgctga
    44581 cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct tccatcacga
    44641 ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc tgatgtggaa
    44701 tttcccattt tgcgccttgc agtccgccaa taaacatcgt tcttttcccc tgatattcac
    44761 acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcgaaac aggattgtgc
    44821 tgtcaccttc tttatccatt gagatcatca tggtcagctc aagcgtgaac tgctcgccgt
    44881 ttttgccttc cagcttcgcc agttgcagcc cgggggtatt caaatataag ctgaattctt
    44941 ccgccgacat acatccgcgg agtaacgcat aatggtaacg taacgcctcc agcaattgct
    45001 tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt cgcggttggc
    45061 gcgttaacaa cacattgaga ttaggccagt gggaaagttc gttcatccac tcaacgctta
    45121 aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg cgatgccaga
    45181 atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg ctgctgagag
    45241 attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa tcaatggtta
    45301 tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg tgattactaa
    45361 cgtaaatttt agggttttgt tgatgtttcg ttgaagttaa tgacccggat tggcatatgg
    45421 agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct
    45481 ggtgatagtc atcgccggac tgattacgtt atggagaatt cttaacgcac ccgtgccgac
    45541 ttatcagaca ctgattgtgc gccccggtga tttacagcaa agcgtgctgg cgaccggaaa
    45601 gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt tgaaaactct
    45661 gttggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtta ttgatcctga
    45721 acaggctgaa aaccagatta aggaggtcga agcaacgctg atggagctac gtgcgcagcg
    45781 gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc agcaacgtct
    45841 ggcacaaacg caggcggttt cacagcagga tctcgacacc gccgcgacgg agatggctgt
    45901 gaaacaggcg caaattggca ccattgacgc gcaaatcaag cgcaatcagg cctctctcga
    45961 tacggctaaa accaatctcg attacactcg catcgttgcc ccgatggccg gggaagtcac
    46021 gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac cgaacattct
    46081 gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag cggatgtaat
    46141 ccacctgaag ccggggcaaa aagcctggtt tacggtactt ggcgatccac tgacgcgcta
    46201 cgaggggcaa atcaaggatg tactaccgac gccggaaaag gttaacgacg ctattttcta
    46261 ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata tgactgcgca
    46321 agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt cggcgttagg
    46381 cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg aaacacgcga
    46441 gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca aagggcttga
    46501 agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgcacaat gacgcctttg
    46561 ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt tgaggtgctg
    46621 aagggcatca ccctcgatat ttatgccggt gagatggtag cgattgttgg cgcttcaggt
    46681 tccggtaaat cgaccctgat gaatattctc ggctgtctgg ataaggccac cagcggcacc
    46741 tatcgcgtcg ccggtcagga tgttgccaca ctggacgccg atgcgctggc gcaactgcgc
    46801 cgcgagcatt tcggctttat tttccagcgt taccatttac tttcgcattt aaccgccgag
    46861 cagaacgttg aagtacccgc cgtctatgcc ggtcttgagc ggaaacagcg gctgcttcgc
    46921 gcccaggggt tactgcaacg actggggctg gaagaccgta cagagtatta tccggcacag
    46981 ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg cattgatgaa cggtggtcag
    47041 gtaattcttg ccgatgaacc aaccggcgca ctggacagcc attctggcga agaggtgatg
    47101 gcgatcctgc atcagctgcg cgatcgtggg catacggtga ttatcgtcac ccacgatccg
    47161 caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat tgtgcgcaat
    47221 cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg agcctgttgt caacacggtg
    47281 tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc atggcgggcg
    47341 ctggcagcga ataaaatgcg tactttactg accatgctgg ggattattat cggtattgcg
    47401 tcggtggttt ccattgtcgt ggtgggtgac gccgccaaac aaatggtgct ggcggatatt
    47461 cgttctattg gtacgaatac tattgatgtc tatcccggga aagattttgg cgatgacgat
    47521 ccgcaatatc agcaggcgct gaagtacgac gacttaatcg ccatccaaaa acaaccgtgg
    47581 gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa caatgttgat
    47641 gttgctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg catgaccttc
    47701 agtgaaggaa acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt cgtggttctc
    47761 gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg cgaggtgatt
    47821 ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt
    47881 ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg gcgagttatg
    47941 ggccagtcgt ggctgaactc cattactgtc agggtaaaag aaggatttga cagcgccgag
    48001 gcggaacagc aactcacgcg tttactttca ctgcgccacg gaaagaagga tttctttacc
    48061 tggaacatgg acggcgtctt gaaaactgtt gaaaagacca cacgtacttt acaactgttt
    48121 ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat gaatattatg
    48181 ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt aggtgcgcga
    48241 gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac tggtttgcct ggtcggtggc
    48301 gcgttgggaa taactctgtc actgttaatt gctttcacct tgcagctttt cttacccggc
    48361 tgggagattg gtttttcacc gttggcactg ctgctggcgt ttctctgctc gacggccacc
    48421 gggattttat ttggatggtt acccgcacga aatgcggcac gactggatcc agtagatgct
    48481 ctggcacgag agtaattttt gagataaaaa tgccagccga tcgggctggc attttgcctt
    48541 taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt cgacgggcac
    48601 aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa cggattgtcc
    48661 agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga aaatatcttc
    48721 gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc acttaacagt
    48781 acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg gaataaaccg
    48841 gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt tagagaatta
    48901 gggcgagccg tcaagcgttt gacaggggcc agggggcagg tatgaatcaa aaatttgaag
    48961 cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat agatgatagt
    49021 tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg cagggcgttc
    49081 tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg actggctgga
    49141 ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat ctatgtataa
    49201 agtgatatta gtcaatgatg attacactcc gatggagttt gttattgacg tgttacaaaa
    49261 attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc actaccaggg
    49321 gaaggccatt tgcggagtct ttaccgccga ggttgcagaa accaaagtgg cgatggtgaa
    49381 caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag cctgaatgca
    49441 ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca gtttaaatat
    49501 ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc acttgttact
    49561 ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgttctg tggatttggt
    49621 tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc tgcctgccag
    49681 tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc aacgtgcggt
    49741 cttccatgtc cagtcctccg gtcgcaatga ggtaaccggt gcaaacgttc tggtcgctat
    49801 ctttagcgaa caggagtcgc aggcggcata tctgttgcgt aaacatgaag tcagccgtct
    49861 cgatgtggtg aactttatct ctcatggcac gcgtaaagac gagccgacac agtcttctga
    49921 tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta tggagaattt
    49981 cacgacgaac ctgaatcagc ttgcgcgcgt gggcggaatc gacccactga ttggtcgtga
    50041 gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca acccgctgct
    50101 ggtgggggaa tctggtgtcg gtaaaaccgc gattgcggaa ggtcttgcct ggcgaattgt
    50161 tcagggcgat gtgccggaag tgatggctga ctgtacgatt tactctctcg atatcggttc
    50221 tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa cgttttaaag cgttgctcaa
    50281 gcagctggag caggacacta acagcatcct gtttattgat gagatccaca ccattatcgg
    50341 tgcgggggca gcgtctggtg gccaggtcga tgcggctaac ctgatcaaac cgttgctctc
    50401 cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca acattttcga
    50461 gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac cgtcgatcga
    50521 agaaactgtt caaatcatca atggcctgaa accgaagtat gaagcgcacc acgacgtgcg
    50581 ttataccgca aaagcggtgc gtgcagcggt agagctggcg gtgaaataca ttaacgatcg
    50641 tcatctgccg gataaagcca ttgacgttat cgacgaagcg ggcgctcgcg cacgcctgat
    50701 gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtccg tggtggcccg
    50761 tattgcgcgc attccagaga agagtgtttc tcagagtgac cgcgataccc tgaaaaacct
    50821 cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg cgctgactga
    50881 agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggttg gttcgttcct
    50941 gtttgccggc cctaccgggg tcgggaaaac agaggtgacg gtacagcttt cgaaagcgtt
    51001 gggcattgag ctgctgcgct ttgatatgtc cgagtatatg gaacgccata ccgtcagccg
    51061 tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggaggtttgc tgactgatgc
    51121 ggtcatcaag catccacatg cggtgctgtt gctggacgaa atcgagaaag cgcatccgga
    51181 cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata acaacggacg
    51241 caaagcggac ttccgtaacg tggtgctggt gatgaccacc aacgccgggg tacgtgaaac
    51301 tgagcgtaaa tccattggtc ttatccacca ggataacagc accgatgcga tggaggagat
    51361 caagaagatc tttacaccgg aattccgtaa ccgtctcgac aacattatct ggtttgatca
    51421 tctgtcaacc gacgtgatcc atcaggtggt ggataaattc atcgtcgagt tgcaggttca
    51481 gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact ggctggccga
    51541 gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc aggacaacct
    51601 gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg gtggacggcg gtcaggtcac
    51661 cgtcgcgctg gataaagaga aaaatgagct gacttacgga ttccagagtg cacaaaagca
    51721 caaggcggaa gcagcgcatt aatctgattg ttaggtaggt tggtcaagtc cgtaatctcg
    51781 aaagaggtta cggacttttt gtttatgggg tggaggaggt tcagaccctt tttttaatga
    51841 tgatggtaag ttgttgataa ttagtgctgc gggaaggtaa ggataaaaaa gggtgctgca
    51901 ggagaatggg atggttttgc tttattaaca acgggctaaa cgtgtagtat ttgagttcac
    51961 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
    52021 tgccgtacag acagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
    52081 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
    52141 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
    52201 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
    52261 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
    52321 acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
    52381 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
    52441 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
    52501 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
    52561 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
    52621 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
    52681 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
    52741 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
    52801 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
    52861 ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
    52921 cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat tattcggcaa
    52981 tcgtccgagg cgcatttgat tgagataatt aaggtaatcc cggcggggaa tttcgcaggc
    53041 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgacc
    53101 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
    53161 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
    53221 atcttcacgc cagacttcaa tggagtgggc atggccgagt tcgtgaaggc gatggtaggc
    53281 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat
    53341 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
    53401 catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat cgggcgacca
    53461 ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
    53521 caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct cacgtaatgc
    53581 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
    53641 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt gaactggtaa
    53701 taacgcccct gtctggcaag cagctctgcg tgagtacctt gctcaataat ttgcccgttg
    53761 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
    53821 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
    53881 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
    53941 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
    54001 ctgttgagac ctgcatcctc gagcagcttc tccaggccaa cgcgacgcaa gctctccgcc
    54061 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    54121 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    54181 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc
    54241 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
    54301 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    54361 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    54421 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
    54481 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    54541 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    54601 gccatccaca gcatcaggat caccgctaac gcgccaatga gtagcattat cgcttgcgac
    54661 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    54721 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    54781 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    54841 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
    54901 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc
    54961 acaaaagcgc ccaccagcgg cgagataacg cgcagataaa gatgatcgag cgtatcaaca
    55021 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    55081 aggggcagca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    55141 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc tgcgccacgc
    55201 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgcaaccgct
    55261 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtgaca
    55321 attgccagca cgataccaag acttaacatc catttatgac gtttatacag tgccagatag
    55381 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaattt ggcgaatggg
    55441 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    55501 acccaaatga tatcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    55561 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    55621 ctggcagcgg gttcatccaa tagtaatagc gagcagggat ttagtaacgc acgggccacc
    55681 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca
    55741 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    55801 aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa tgttgctgcc
    55861 ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    55921 tcgcgtaatt ctatcccgtt gattcgtaat gatccctgat atgagagaaa accagaaagc
    55981 gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc acgttggcct
    56041 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    56101 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
    56161 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    56221 gctttagcat gataaaacgt accgagatcg cgcaatggct ggaaaaactc tggcgcaagg
    56281 atcagggcca gaaaacccgc agccagcgtc acaccggtat cgtagtgacc aaaatccagc
    56341 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    56401 aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt ccgttggcgg
    56461 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    56521 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    56581 aagttacgcc ggttagcatc ggcagccccc atcccaacca gcgccataaa caacggaatt
    56641 aacggtgctg tgcccagcag aatgagcgcc gcagcccagt tagaggggaa gatcgccacc
    56701 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    56761 tcatgcatat cgtcaatttg ctcgagtacc aacgtcgccc agctccccgc aggtttaccc
    56821 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    56881 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc
    56941 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    57001 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    57061 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    57121 tgttttaacc agcgggttaa ctctttttga cgggatttat tcattgcacg cttagcaggt
    57181 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    57241 agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    57301 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta ccggccgaag
    57361 taatggcctg acgataaata tgatccatca cgtcacccgc ggcaaagacg ccaggaatgc
    57421 tggtctgggt ggcattacca tgaatacccg actgcacttt gatgtagccg ttttccagtt
    57481 ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc gatagcaaca aacagaccag
    57541 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    57601 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    57661 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    57721 ggcggtgaat aagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    57781 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    57841 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    57901 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    57961 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    58021 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    58081 ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    58141 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    58201 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    58261 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    58321 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    58381 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    58441 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    58501 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    58561 ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    58621 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    58681 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    58741 cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca ataataatgg
    58801 tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    58861 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    58921 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    58981 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    59041 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    59101 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    59161 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    59221 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    59281 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    59341 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    59401 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    59461 attgacaaag ttaagtagcg gccgtcgcct tctggaagcg ttgctgatcc ttattgtcct
    59521 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    59581 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggctggc
    59641 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    59701 cggttgttgg tttgcctggc gtcatcagtc cagtgacgaa tacattgatt attttgccgt
    59761 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    59821 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    59881 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    59941 tgcgggcctg acgctgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    60001 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    60061 cgatgaagat gaatatgaag atgacgaaga gtatgaagat gaaaaccacg gcaaacagca
    60121 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    60181 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    60241 ggatgatgac gaagagatta tctacactgc acgcggtgtg gctgccgacc cggacgacgt
    60301 cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    60361 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cgacacaaag
    60421 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    60481 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    60541 tattgctcct gcaccggaag gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca
    60601 atataatgag ccgctgcaac aaccagtaca gccgcagcag ccgtattatg cacctgcagc
    60661 tgaacaacct gcgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    60721 gtattacgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    60781 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc
    60841 cgctcaggag ccgttgtacc aacagccgca atccgttgaa cagcagcctg ttgtggagcc
    60901 tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt
    60961 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaat cgattccaga
    61021 accggttaaa gaaccagaac cgatcaaatc ttcgttgaaa gcgccttctg ttgcagcagt
    61081 acctccagta gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    61141 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    61201 tggaccgcgt ccgcaagtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat
    61261 ccgcgtgcca actcgtcgtg aactggcgtc ttacggtatt aagctgccct cacagcgtgc
    61321 ggcggaagag aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa
    61381 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    61441 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    61501 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    61561 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    61621 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    61681 acagcagccg caacagccgg ttgcgccaca gcagcaatat cagcagccgc aacagccggt
    61741 tgcgccgcag ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca
    61801 gcagccgcaa caaccggttg cgccacagcc gcagtatcag cagccgcaac aaccggttgc
    61861 gccgcagccg cagtatcagc agccgcaaca accggttgcg ccgcagcagc agtatcagca
    61921 gccacaacag ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg
    61981 taatggcgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
    62041 gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg
    62101 cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
    62161 ggggccggtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat
    62221 ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    62281 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    62341 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    62401 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    62461 cttgttggtt gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct
    62521 gagcatgctt tataaagcac agccagaaga tgtacgtttc atcatgatcg acccgaaaat
    62581 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat
    62641 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    62701 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    62761 tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    62821 gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct
    62881 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    62941 tgccgcgggt atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg
    63001 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    63061 acgtaccatt cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta
    63121 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    63181 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    63241 cacttccgac agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga
    63301 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    63361 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    63421 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc
    63481 gccgtttgac taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct
    63541 atcaactgaa aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag
    63601 agtagaggga actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg
    63661 ccatcacctg tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa
    63721 gcgatctgaa aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag
    63781 tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc
    63841 caaacttatt caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta
    63901 aaacactgtg gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg
    63961 ccaccggtaa tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt
    64021 acaatatcaa acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc
    64081 tgaagcagtt caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg
    64141 agcaggacga tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg
    64201 cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga
    64261 ggcacctgag tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc
    64321 gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg
    64381 gggaagccgt tgccgcgcgc tatcgaagcc gggcatttac attctatgat cctctggggg
    64441 ccaccgggta ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat
    64501 gtggaacgta tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc
    64561 gcccggcaaa accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga
    64621 ttcaacaaaa gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt
    64681 attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc
    64741 cgtgtctatc tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg
    64801 atggaagaca agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga
    64861 cgcgccattg ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg
    64921 atggcggata tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg
    64981 accgaaatcg ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat
    65041 ctgatttccg cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg
    65101 tatgcgcgaa ttattaccgc cggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg
    65161 atagcgtctg aagatgtggg taatgctgat ccacgagcga tgcaggtggc aattgcggcc
    65221 tgggattgct ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt
    65281 gtttacctag cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg
    65341 gcggatgctc gcgaacgtcc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg
    65401 aaattaatga aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac
    65461 gcttatgctg ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc
    65521 ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag
    65581 gatcaaaata gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact
    65641 gtatccctgt ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata
    65701 agcatgctcg atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca
    65761 cgccggggct ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta
    65821 ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc
    65881 caggcgaaag cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc
    65941 gaagagctgg atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc
    66001 gcgctaacca tccctaactt gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac
    66061 aacgttgaag tcagccgctg gggcaccccg cgtgagtttg actttgaagt tcgtgaccac
    66121 gtgacgctgg gtgaaatgca ctctggcctc gactttgcag ccgcagttaa gctgacaggt
    66181 tcgcgctttg tggtgatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt
    66241 atgctggatc tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt
    66301 aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat
    66361 actcgtccgc tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa
    66421 gttccgctga ccaacctggt acgcggtgaa atcattgatg aagatgatct gccaattaag
    66481 atgaccgccc acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt
    66541 ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa
    66601 gactcaatgg cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcaactgctg
    66661 ggcctaccgt accgtaaaat tatcctttgt accggcgaca tgggctttgg cgcttgcaaa
    66721 acttacgacc tggaagtatg gatcccggca cagaacacct accgcgagat ctcttcctgc
    66781 tccaacgttt gggatttcca ggcacgtcgt atgcaggcac gttgtcgcag caagtcggac
    66841 aagaaaaccc gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg
    66901 gttgcggtta tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg
    66961 cgtccgtata tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa
    67021 agcgcctgcg ggcgcttttt ttgtctccct ttgataccga acaataatta ctcctcactt
    67081 acacgtaata ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga
    67141 tgtatacaag cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact
    67201 ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt
    67261 attggctgct gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc
    67321 aatggccagc agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc
    67381 cattccaaca aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag
    67441 tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa
    67501 taccggcgat gacaattacg acggcctgca ccaggttcgc gcctgtctgc gtgggcgttc
    67561 catgcgtcgc cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc
    67621 gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac
    67681 caatatgcag cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac
    67741 cggtacgctg ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg
    67801 cctgatgaac tgctgcggcg gttatcttaa ccattacggt gactactctt ctgcgcaaat
    67861 tgcggaaggt ttgaactata cctacggcgg ctgggcagat ggtaacagcc cgtcggatat
    67921 cgaaaacagt aaattggtag tgctgtttgg taataaccca ggcgaaacgc gaatgagtgg
    67981 cggtggggtg acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat
    68041 catcgatccg cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg
    68101 tccaggaaca gatgccgcac tggttaacgg tctggcgtat gtcatgatca ccgaaaacct
    68161 ggtggatcag gcattccttg ataaatattg cgttggctac gatgagaaaa ccctgcccgc
    68221 tagcgcgccg aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt
    68281 ggctaaaaca ccgcaatggg cctcgcaaat caccggtatc ccggcagaga aaatcattca
    68341 actggcacgg gaaatcggca gtaccaaacc ggcgtttatc agccagggat gggggccaca
    68401 gcgccacgcc aacggcgaaa tcgcgactcg cgctatctcg atgctggcga ttctgaccgg
    68461 taacgtcggt attaacggag gcaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt
    68521 tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac
    68581 cgatgccatt gaacgtggcc cggaaatgac agcgctgcgt gatggtgtac gcgggaaaga
    68641 taagctggat gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tgattaacca
    68701 gcattctgaa atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat
    68761 tgtggttatc gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga
    68821 ctgcaccgct tccgaacaga tggactttgc actggatgca tcctgcggga atatgtctta
    68881 cgtgattttc aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat
    68941 gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtacaca
    69001 ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac
    69061 gtttgaagag ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt
    69121 tgcttataaa gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa
    69181 aattgagatt tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg
    69241 cgatgtgatc gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct
    69301 gaacaaacag tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac
    69361 ttacggcaac gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct
    69421 tgatgcgcaa aaacgcggta ttaacaacgg cgataaagtc aggatcttta acgatcgtgg
    69481 tgaggttcat attgaggcga aagtgacgcc acgaatgatg ccgggtgtgg tcgcactggg
    69541 tgaaggtgcc tggtatgacc cggatacaaa acgtgtcgat aagggcggtt gtatcaacgt
    69601 actgaccact caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt
    69661 tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt
    69721 ccagccgttg caccggttgc aaaacctgcg agctggcctg taaagactac aaagatttga
    69781 cgccagaagt cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata
    69841 acggtgtctg gcaccagaac gtgtttgcct actatctgtc gatttcatgt aaccactgcg
    69901 aagatccggc ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt
    69961 ttgttgtggt cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt
    70021 atggcgcacc gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg
    70081 accgtgttgc ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg
    70141 atttcggccc tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt
    70201 tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc
    70261 cgaccgggga taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga
    70321 tggcatgaat ggccgctgat gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt
    70381 atcgttctgg ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt
    70441 atcgcctgca tgtttggttt atgggtgctg atgggcattg gctttatcgc ctctatgctc
    70501 catcttggtt caccaatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc
    70561 agtaacgaaa tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg
    70621 gcaatgctga aaaagctgtc accggcattg cgtacgctgt ggctgatagt gacgatggtt
    70681 cttggcgtca tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg
    70741 acctggtaca gcatctggac gccgatgggc ttcttcctga cgatgtttat gggcggcccg
    70801 ttactgggtt acctgttgtt gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg
    70861 ccagccattt ctgtactggc actggtggtg agtggcgtga tgtcagtgat gcagggcaca
    70921 gagctggcaa ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt
    70981 gcgctgatgt cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca
    71041 cagctaaaag gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg
    71101 gcaggggaat taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc
    71161 gtcgcaagct aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc
    71221 gttaacgtgt cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac
    71281 gtcgccagtc cttcaatatc attacgccag tcacggtgca gctcgcacgc cacgccgaac
    71341 catgtcatca attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt
    71401 tcattaaagg tccctgacgc gtcagtcacg acaaagacgt caaacccttc ttcaatggct
    71461 gaaagcgccg ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt
    71521 ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatatttccc
    71581 gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatggaccg
    71641 ttaggaccgg tttcaaaact ggtggtaaga atagtaggta agttgaaata cttagccagg
    71701 tcacccagcg ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa
    71761 agtaaaccag cctgatgatc aacaagcaaa acggcagcat catttttatc aagacgaaca
    71821 tacggtttgg tcatcctctt ctcctttcga gagtcagtgg tacgtctgaa atcggtcaga
    71881 cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    71941 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    72001 taatcatctc ttcagcatta gcgttatgaa tgagtaatat aaatcgataa taccgccgct
    72061 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    72121 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    72181 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    72241 ttggctcgcc caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt
    72301 taccggcaac cttgtcggta cattgctgac agggtatgtc attaagcgca ttggctttaa
    72361 ccgcagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    72421 gattggattc tggagctggt tggcttggcg ttttgtcgcg ggcgtcggct gtgccatgat
    72481 ttgggtggtt gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt
    72541 gcttgctgcg tatatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag
    72601 caaagtttca accgagctga tgtccgtatt gccgtgggtt acaggtttga cgttggcagg
    72661 gatcttaccg ctgttgttta cgcgtgtgct gaatcagcag gctgaaaacc atgattcgac
    72721 gtcaattacg tcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    72781 ctcaggaatc gttctgggat ctctatatgg cctgatgccg ctgtacctca atcacaaagg
    72841 ggtgagcaat gccagcattg gtttctggat ggcggtactg gtcagtgcgg gtatccttgg
    72901 acaatggccg attggacgtc tggcggataa gtttggtcga ctgctggtgt tgcgtgttca
    72961 ggtctttgtc gtcattctcg gcagtatcgc gatgcttagc caggcggcga tggccccagc
    73021 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg
    73081 cgagaaagtt gaacatcatc aactggtggc gatgaaccag gccttactgt tgagctatac
    73141 tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    73201 tttattgttt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa
    73261 cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac
    73321 ttactgtttc acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt
    73381 attcgtttaa aaataaaatg tgcagcaggt tataattttg catttcgcta tttccgcact
    73441 tcttatttgc cgcgcataat ccctcgtttt acagatgccc ctttaatttt ggcgaaggat
    73501 ttgtctatgg ctgggaatgt tcaggaaaaa cagttgcgat ggtacaacat tgcgctgatg
    73561 tcttttatca ctgtctgggg ttttggcaac gttgttaata actatgccaa ccaggggctg
    73621 gtggttgttt tttcatgggt gtttatcttt gcactctatt tcacacctta tgcgctaatt
    73681 gttggtcagt taggatcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa
    73741 cacacgatgg gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt
    73801 ccctatctgg cacaaaaacc ccaggcaatt ctgattgcgc tcggttgggc gatgaaaggc
    73861 gacggttcgc taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg
    73921 tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct
    73981 gtggcaggga ttgcgatgtt tgttatgtcg ctcctgtatg tggcaatggc ggtaaccgcg
    74041 cctgcgatta ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct
    74101 catatcgact ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca
    74161 gagaagattt ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg
    74221 atgctatgcc tggcggtgat ggttgcggtt tgtgccattc tgggctcact ggcgatgggg
    74281 atgatgtttg actcgcgtaa tatcccggat gacttaatga ctaacggtca atattacgcc
    74341 tttcagaagc tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt
    74401 gcgaataccc tgggacaagt agctgcgctg gtattctcga ttgatgcccc gcttaaagtg
    74461 ctattaggtg atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct
    74521 ggtacgcccg ttaatggcta ttttctgacc ctggtgctgg tggcaattct gattatgctg
    74581 ccgactctcg gcatcggtga tatgaacaat ctctataaat ggctgttgaa tcttaactcg
    74641 gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg
    74701 gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc
    74761 gggatttggt gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg
    74821 gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg
    74881 ctggtaggat taggactgat tttcccgctg ctggcgcgta aagcgaatag taaataatta
    74941 ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc
    75001 gcgcaagccg caggacatat actggcggtt tggataatag atctggaagc ctggacgctc
    75061 tgtgctccag tctcccagta cacattctaa acgaccagta tcaaggtact ccttgatctg
    75121 ttcatagagc agatacccga taccagcacc catcaaaacg gcctccagtt cagcatcaac
    75181 atcatccaga atgatatttc ctgcgacagc aatttccagc tcctttgcaa attgccagtg
    75241 gaaaggtttg ccgctgggat aacgaaaaac aacacattga tgattaagca gatcatgagg
    75301 atggcggggt ttgccatatc ttgcaaagta ctctggcgtt gctgctacac ataatttcac
    75361 tggtgggccg atcgccacgc tgatcatatc tttttcgacg atgccactaa gtcgaacgcc
    75421 agcgtcaaag ccctgttgga cgatatcgac aagagaatcg tcagttgtga gctcgacttt
    75481 aatatcagga tattcacgtg tgaaaccaac cagtaaaggc attaaaaata tacgtgcagc
    75541 tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc agacgaaaat cattcatctc
    75601 atctaacatg atctgaattt cgtcgaaagc tgggcgcaat cgttcataga gattagaacc
    75661 cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat aagcgaattt taagacgttg
    75721 ttccagtgtt ttaatactat ggctaatggc ggacgaggat aagcctaatt catcgcccgc
    75781 tgcacgaaag ctttgattac gggccacggc aaagaaagtg gcaaagtcag acatattcat
    75841 ccgcattagt gaaaatcctt cattaactca tcctgaatca tcacgtttac aggattatgc
    75901 cgcctgcgta aagtgcgctc cagaacttaa cgtggaggta aaattatgca gtctgaacgt
    75961 atttatttgg tatgggccca tcctcgtcat gattcattga ccgcacatat tgctgatgcg
    76021 atccatcagc gggcaatgga gcggaaaata caggtgacgg aactcgattt atatcggcgt
    76081 aatttcaacc cggtgatgac gtcggaagat gaaccagact ggaagaatat ggataaacgt
    76141 tattctccag aggttcatca gctttattca gagctgcttg agcatgacac gttagtggtg
    76201 gtttttcctc tctggtggta cagcttcccg gcaatgctaa aaggatatat tgacagagta
    76261 tggaataatg ggctggctta tggagatggg cacaaattac cattcaataa agttcgttgg
    76321 gtggcgctgg ttggaggaga caaagaatca tttgtccaga tgggctggga aaaaaatata
    76381 agcgattatt taaaaaatat gtgcagttat cttggtattg aagatgccga tgtcactttc
    76441 ttgtgtaata cagtggtatt cgatggggaa gaacttcacg cgagctatta tcagtcgtta
    76501 ttatctcagg tacgggatat ggtagatgca ctataagatg tgttaaaaac gctgtagcag
    76561 aatgaagcgc ggaataaaaa agcggcaact caataaagtt gccgctttac ggggaaatta
    76621 gaacattacc ttatgaccgt actgctcaag aatgcctttc acgcgttcca tggtctcttt
    76681 cttaggtggt ttaacaccgt cgagtttgta ctcttcaccc attgccaccc atttgtgttt
    76741 gcccagctcg tggtagggga ggagttcgat tttctcaacg ttgcccatat cacgggtaaa
    76801 ttcaccgagg cgatgcgctg aatcgtcatc gtcagaccag cctgggacaa caacgtagcg
    76861 gatccacacc ttcacatttt tgttcgccag atatttggcg aactccagcg tgcggtggtt
    76921 ggaaactcca accagatttt ggtggatctc gtcgttcatc tgtttgagat cgagcattac
    76981 caggtcggtt acttccagca gttcatcaat caccggatcg taacgacgaa caaaaccgtt
    77041 ggtgtccaga caggtatgaa tgccttcttt tttgcaggcg cggaaccagt cacgaacaaa
    77101 ctcagcttgt aggattgcct caccgccgga tgcggtaacg ccgccgccgg aagcgttcat
    77161 aaagtggcga taggtcacca cctctttcat taaatcttca acggtaactt ctttaccgcc
    77221 atgcgtatcc caggtgtcgc ggttatgaca atacaggcag cgcatcaggc agccctggaa
    77281 aaaggtgata aagcgaatac ctgggccgtc tacggttcca caggattcaa aggagtgaat
    77341 gcgaccaata actgacattg cggtgtttct ccagatgtgg cccatctgag gccgtgttgg
    77401 tgcgcagctc gaaggctacg tcgagtctgt tttggcagtc accttaaagt atagatagct
    77461 gacaaaaaag gctctcgtgc taaaaaaggc cccactttcg tggagccttt attgtacgct
    77521 ttttactgta cgatttcagt caaatctaat tacatagatt gagtgaaggt acgagtaata
    77581 acgtcctgct gctgttcttt agtcagcgag ttgaaacgta ctgcgtagcc agatacacgg
    77641 atggtcagct gcggatattt ttccgggttt tccatcgcgt cgagcagcat ttcacggttc
    77701 atcacgttaa cgttcaggtg ctgaccacct tcgatggatg cttcgtggtg gaagtaacca
    77761 tccatcagac cagccaggtt ggtcttacga acttcgtcgt ctttacccag tgcgttcgga
    77821 acgatagaga aggtgtagga gataccatct ttagcgtaag caaacggtag tttagcaacg
    77881 gaagtcagag acgctacagc acctttctgg tcacgaccgt gcatcgggtt agcacccggt
    77941 ccgaacggcg cgccagcacg acgaccgtct ggggtgttac cagttttctt accatacaca
    78001 acgttagaag tgatggtcag aacagactga gtcgggatag cgtcacggta ggtgtgcagt
    78061 ttctgaattt tcttcatgaa acgttctacc aggtcaacag ccaggtcatc tacacgcgga
    78121 tcattgttac caaactgcgg gtattcgcct tcgatttcga agtcgatagc cagaccgtct
    78181 tcgtcacgaa tcggtttaac tttcgcatat ttgattgcag acagggagtc agcagcaacg
    78241 gacagaccag cgataccaca cgccatggtg cggataacgt cacggtcgtg cagcgccatc
    78301 agagaggctt cgtagctgta cttgtcgtgc atgtagtgga tgatgttcag tgcagtgatg
    78361 tactgtttag ccagccagtc catgaagtga tccatgcgct ccatcacttc atcatagttc
    78421 aggacatcgc ctttgatcgg ttcagacttc ggaccaacct gcattttcag tttttcgtca
    78481 acgccgccgt tgattgcgta cagcatggtt ttcgccaggt ttgcacgcgc accgaagaac
    78541 tgcatttgtt taccaacgat catcgggctt acgcagcaag caatagcgta gtcatcgttg
    78601 ttgaagtccg gacgcatcag gtcatcgttc tcatactgca gagaagaggt gtcgatggac
    78661 actttagcgg cgaatttctt gaagttcagc ggcagttttt cagaccatag aatggtcatg
    78721 ttcggttccg gagacggacc catggtgtac agggtgttca ggaaacggaa gctgtttttg
    78781 gtaaccaggg tacgaccgtc gaggcccata ccaccgatag attcggttgc ccagatcggg
    78841 tcgccagaga acagttcatc gtattccgga gtacgcagga agcgaaccat acgcagtttc
    78901 atgaccaggt ggtcaaccat ttcctgcgct tcttgttcgg tgatcttgcc agctttcagg
    78961 tcacgttcga tgtacacatc caggaaggtg gaggtacgac cgaaggacat tgcagcaccg
    79021 ttctgagact taacagcagc caggtagccg aagtaagtcc actggatagc ttcctgagcg
    79081 ttggtagccg gaccagagat gtcgtagccg tatttcgcag ccatttcttt catctgaccc
    79141 agagcgcggt gctgttcagc gatttcttcg cgcagacgga tagtctgttc caggtttacg
    79201 ccgttttcca gatcagcctg cagagaggtg aactgagcgt atttgtcttt catcaggtag
    79261 tcgataccgt acagcgcaac gcgacggtag tcaccgatga tacggccacg gccataagca
    79321 tctggcagac cggtcagaac accggattta cggcaacgca ggatgtccgg agtgtaaacg
    79381 tcgaacacgc cctggttgtg agttttacgg tattcagtga agattttttt gatcatcggg
    79441 tccagttcgc ggttgtacgc tttgcaggaa ccctcgatca ttttgatacc accgaacggg
    79501 ataagagcac gtttcagcgg agcttcagtc tgtagaccaa caactttttc caacgctttg
    79561 ttgatgtagc cagcgtcgtg agaggtgatg gtggaagcaa cagcggtgtc aaagtcaact
    79621 ggcgcgtgag tgcggttttc cagtttaacg ccttccatta ctttgtccca cagggtggtg
    79681 gtcgcttcag tagcgccagc caggaaggac tcgtcaccct cgtacggagt gtagtttttc
    79741 tgaatgaagt cacggacgtt tacttcattc tgccagtcac ctttggtaaa accttcccag
    79801 gctgtggcta acttttcatt aagctcggac atgtaacacc taccttctta agtggatttt
    79861 ttatttactg cgtacttcga caaccattaa tggtgatcgt tttcacgcag gtaaatgacc
    79921 cagtatgtca acccaaccaa caaaccaccg ccgataatat taccgatcgt aaccggaatc
    79981 aggttatcag tgatgaagtt catcacggtc aggtgagaaa aattttccgg tgcagaaccg
    80041 acagcggtcc agaattccgg agatgcgaag tcgcggatta caatacccat cgggatcata
    80101 aacatgtttg cgatactgtg ctcaaaaccg ctggcaacaa acatcgcgac cggcagcacc
    80161 ataatgaacg ctttgtccat caggctacgg ccagaataac tcatccatac tgccagacat
    80221 accatcaggt ttgccaggat accaaggcag acggcctcaa taaaagtatg gtgcactttg
    80281 tggtcggcgg tttgtaggac gtttagtccc cattgaccat ttgcggtcat atactcgccg
    80341 gaaagccaca ttaaaagtac aaacagcagt gcgccgacca ggttgccaaa atagacattt
    80401 agccagtttt tcgccaactg accccaggtg atgcgcccac tcgccttagc aacaacaatc
    80461 aacactgtgg aagtaaagag atcggctccg cagacaacac aaagaatcag ccccagagag
    80521 aagcaaatgc cgccaaccag ttttgccatg ccgaagggca ttgtgcctgt gccagtggtt
    80581 gctgtgatat agaagacgaa tgcgattgag atgaaaacac cggcggtaat cgccagatag
    80641 aaagtcttaa gcggatgttt cgttgcttta tagacacccg cctcttcggc cactttggcc
    80701 attgcagcag gaagtaaaag atcaaaaggg ttgtcagctt tcacactaac tctctcttta
    80761 ttaagtcggc gacgagatac taacaaagca ttatagatga gaaattgata tagatcatat
    80821 ctcgcctggc ttatagg
//
LOCUS       DAZFFI010000009        79375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFFI010000009 DAZFFI010000000
VERSION     DAZFFI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.009"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..239)
                     /locus_tag="ACHJ12_000796"
                     /pseudo
     CDS             complement(1..239)
                     /locus_tag="ACHJ12_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(271..1287)
                     /gene="lplA"
                     /locus_tag="ACHJ12_000797"
     CDS             complement(271..1287)
                     /gene="lplA"
                     /locus_tag="ACHJ12_000797"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4988628.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(1315..1959)
                     /gene="ytjB"
                     /locus_tag="ACHJ12_000798"
     CDS             complement(1315..1959)
                     /gene="ytjB"
                     /locus_tag="ACHJ12_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO4988629.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2065..3033
                     /gene="serB"
                     /locus_tag="ACHJ12_000799"
     CDS             2065..3033
                     /gene="serB"
                     /locus_tag="ACHJ12_000799"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4988630.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            3082..4464
                     /gene="radA"
                     /locus_tag="ACHJ12_000800"
     CDS             3082..4464
                     /gene="radA"
                     /locus_tag="ACHJ12_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4988631.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4485..5717
                     /gene="nadR"
                     /locus_tag="ACHJ12_000801"
     CDS             4485..5717
                     /gene="nadR"
                     /locus_tag="ACHJ12_000801"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4988632.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(6024..7691)
                     /gene="ettA"
                     /locus_tag="ACHJ12_000802"
     CDS             complement(6024..7691)
                     /gene="ettA"
                     /locus_tag="ACHJ12_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4988633.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            7902..9839
                     /gene="sltY"
                     /locus_tag="ACHJ12_000803"
     CDS             7902..9839
                     /gene="sltY"
                     /locus_tag="ACHJ12_000803"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4988634.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFVRTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDKQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            9929..10255
                     /gene="trpR"
                     /locus_tag="ACHJ12_000804"
     CDS             9929..10255
                     /gene="trpR"
                     /locus_tag="ACHJ12_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4988635.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(10341..10862)
                     /gene="yjjX"
                     /locus_tag="ACHJ12_000805"
     CDS             complement(10341..10862)
                     /gene="yjjX"
                     /locus_tag="ACHJ12_000805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4988636.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            10914..11561
                     /gene="gpmB"
                     /locus_tag="ACHJ12_000806"
     CDS             10914..11561
                     /gene="gpmB"
                     /locus_tag="ACHJ12_000806"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4988637.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(11558..12427)
                     /gene="robA"
                     /locus_tag="ACHJ12_000807"
     CDS             complement(11558..12427)
                     /gene="robA"
                     /locus_tag="ACHJ12_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4988638.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            12638..13111
                     /gene="creA"
                     /locus_tag="ACHJ12_000808"
     CDS             12638..13111
                     /gene="creA"
                     /locus_tag="ACHJ12_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4988639.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13124..13813
                     /gene="creB"
                     /locus_tag="ACHJ12_000809"
     CDS             13124..13813
                     /gene="creB"
                     /locus_tag="ACHJ12_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4988640.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            13813..15237
                     /gene="creC"
                     /locus_tag="ACHJ12_000810"
     CDS             13813..15237
                     /gene="creC"
                     /locus_tag="ACHJ12_000810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4988641.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            15295..16647
                     /gene="creD"
                     /locus_tag="ACHJ12_000811"
     CDS             15295..16647
                     /gene="creD"
                     /locus_tag="ACHJ12_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4988642.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(16707..17423)
                     /gene="arcA"
                     /locus_tag="ACHJ12_000812"
     CDS             complement(16707..17423)
                     /gene="arcA"
                     /locus_tag="ACHJ12_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4988643.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            17519..17659
                     /gene="yjjY"
                     /locus_tag="ACHJ12_000813"
     CDS             17519..17659
                     /gene="yjjY"
                     /locus_tag="ACHJ12_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4988644.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            18059..18745
                     /gene="yjtD"
                     /locus_tag="ACHJ12_000814"
     CDS             18059..18745
                     /gene="yjtD"
                     /locus_tag="ACHJ12_000814"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4988645.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            18959..19024
                     /gene="thrL"
                     /locus_tag="ACHJ12_000815"
     CDS             18959..19024
                     /gene="thrL"
                     /locus_tag="ACHJ12_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4988646.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    18960..19079
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            19105..21567
                     /gene="thrA"
                     /locus_tag="ACHJ12_000816"
     CDS             19105..21567
                     /gene="thrA"
                     /locus_tag="ACHJ12_000816"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4988647.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            21569..22501
                     /gene="thrB"
                     /locus_tag="ACHJ12_000817"
     CDS             21569..22501
                     /gene="thrB"
                     /locus_tag="ACHJ12_000817"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4988648.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            22502..23788
                     /gene="thrC"
                     /locus_tag="ACHJ12_000818"
     CDS             22502..23788
                     /gene="thrC"
                     /locus_tag="ACHJ12_000818"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4988649.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            24002..24298
                     /gene="yaaX"
                     /locus_tag="ACHJ12_000819"
     CDS             24002..24298
                     /gene="yaaX"
                     /locus_tag="ACHJ12_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4988650.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(24488..27184)
                     /gene="mgtA"
                     /locus_tag="ACHJ12_000820"
     CDS             complement(24488..27184)
                     /gene="mgtA"
                     /locus_tag="ACHJ12_000820"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4988651.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(27325..27378)
                     /gene="mgtL"
                     /locus_tag="ACHJ12_000821"
     CDS             complement(27325..27378)
                     /gene="mgtL"
                     /locus_tag="ACHJ12_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4988652.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            27563..28510
                     /gene="treR"
                     /locus_tag="ACHJ12_000822"
     CDS             27563..28510
                     /gene="treR"
                     /locus_tag="ACHJ12_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO4988653.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            28629..30050
                     /gene="treB"
                     /locus_tag="ACHJ12_000823"
     CDS             28629..30050
                     /gene="treB"
                     /locus_tag="ACHJ12_000823"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4988654.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            30100..31755
                     /gene="treC"
                     /locus_tag="ACHJ12_000824"
     CDS             30100..31755
                     /gene="treC"
                     /locus_tag="ACHJ12_000824"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4988655.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            32149..34287
                     /gene="nrdD"
                     /locus_tag="ACHJ12_000825"
     CDS             32149..34287
                     /gene="nrdD"
                     /locus_tag="ACHJ12_000825"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4988656.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            34445..34909
                     /gene="nrdG"
                     /locus_tag="ACHJ12_000826"
     CDS             34445..34909
                     /gene="nrdG"
                     /locus_tag="ACHJ12_000826"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4988657.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(34954..35340)
                     /gene="cybC"
                     /locus_tag="ACHJ12_000827"
     CDS             complement(34954..35340)
                     /gene="cybC"
                     /locus_tag="ACHJ12_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4988658.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(35524..36876)
                     /gene="pmbA"
                     /locus_tag="ACHJ12_000828"
     CDS             complement(35524..36876)
                     /gene="pmbA"
                     /locus_tag="ACHJ12_000828"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4988659.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            36970..37521
                     /gene="yjgA"
                     /locus_tag="ACHJ12_000829"
     CDS             36970..37521
                     /gene="yjgA"
                     /locus_tag="ACHJ12_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4988660.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(37677..39050)
                     /gene="mpl"
                     /locus_tag="ACHJ12_000830"
     CDS             complement(37677..39050)
                     /gene="mpl"
                     /locus_tag="ACHJ12_000830"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4988661.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQLGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            39226..40224
                     /gene="fbp"
                     /locus_tag="ACHJ12_000831"
     CDS             39226..40224
                     /gene="fbp"
                     /locus_tag="ACHJ12_000831"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4988662.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(40257..41252)
                     /gene="yjfF"
                     /locus_tag="ACHJ12_000832"
     CDS             complement(40257..41252)
                     /gene="yjfF"
                     /locus_tag="ACHJ12_000832"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4988663.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIVQR"
     gene            complement(41239..42261)
                     /gene="ytfT"
                     /locus_tag="ACHJ12_000833"
     CDS             complement(41239..42261)
                     /gene="ytfT"
                     /locus_tag="ACHJ12_000833"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4988664.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVVILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(42275..43777)
                     /gene="ytfR"
                     /locus_tag="ACHJ12_000834"
     CDS             complement(42275..43777)
                     /gene="ytfR"
                     /locus_tag="ACHJ12_000834"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4988665.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(43917..44873)
                     /gene="ytfQ"
                     /locus_tag="ACHJ12_000835"
     CDS             complement(43917..44873)
                     /gene="ytfQ"
                     /locus_tag="ACHJ12_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4988666.1"
                     /translation="MWKRLLIVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            45183..45713
                     /gene="ppa"
                     /locus_tag="ACHJ12_000836"
     CDS             45183..45713
                     /gene="ppa"
                     /locus_tag="ACHJ12_000836"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4988667.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(46171..47166)
                     /locus_tag="ACHJ12_000837"
     CDS             complement(46171..47166)
                     /locus_tag="ACHJ12_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4988668.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(47233..47532)
                     /locus_tag="ACHJ12_000838"
     CDS             complement(47233..47532)
                     /locus_tag="ACHJ12_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4988669.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGQSEAESSHSGKKTG"
     gene            47640..48065
                     /locus_tag="ACHJ12_000839"
     CDS             47640..48065
                     /locus_tag="ACHJ12_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO4988670.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            48096..48587
                     /locus_tag="ACHJ12_000840"
     CDS             48096..48587
                     /locus_tag="ACHJ12_000840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988671.1"
                     /translation="MIKKYSEHGSKAGEAIAIDEDCLITGDPWLATERDLEGKTFFVR
                     AIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKKINLDYMWPI
                     VQREIMRRKSEGFDVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEKYIVEQVRDL
                     VCL"
     gene            complement(48565..48888)
                     /locus_tag="ACHJ12_000841"
     CDS             complement(48565..48888)
                     /locus_tag="ACHJ12_000841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988672.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALMKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            48996..49334
                     /locus_tag="ACHJ12_000842"
     CDS             48996..49334
                     /locus_tag="ACHJ12_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4988673.1"
                     /translation="MTQRRSFRTGTERHANRFSTSASRSNMHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            49354..49596
                     /locus_tag="ACHJ12_000843"
     CDS             49354..49596
                     /locus_tag="ACHJ12_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4988674.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            49593..49718
                     /locus_tag="ACHJ12_000844"
     CDS             49593..49718
                     /locus_tag="ACHJ12_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988675.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            49798..50076
                     /locus_tag="ACHJ12_000845"
     CDS             49798..50076
                     /locus_tag="ACHJ12_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988676.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTKKTLTEITNALDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            50088..50339
                     /locus_tag="ACHJ12_000846"
     CDS             50088..50339
                     /locus_tag="ACHJ12_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988677.1"
                     /translation="MNSIFTATRRTLLTHFTDAAGREFMVESYLITTTTPCPSDADYL
                     YIHLADGTQITAIASTVREVMTIRGAWKSETQAHGELRP"
     gene            50336..50548
                     /locus_tag="ACHJ12_000847"
     CDS             50336..50548
                     /locus_tag="ACHJ12_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988678.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            50574..50840
                     /locus_tag="ACHJ12_000848"
     CDS             50574..50840
                     /locus_tag="ACHJ12_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4988679.1"
                     /translation="MTILDYIAANPGCSGSEIATALNTQTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            50837..51136
                     /locus_tag="ACHJ12_000849"
     CDS             50837..51136
                     /locus_tag="ACHJ12_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988680.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTTPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            51148..51528
                     /locus_tag="ACHJ12_000850"
                     /pseudo
     CDS             51148..51528
                     /locus_tag="ACHJ12_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            51459..51686
                     /locus_tag="ACHJ12_000851"
     CDS             51459..51686
                     /locus_tag="ACHJ12_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4988681.1"
                     /translation="MANRKQQRARAERLHIHTEINRRLHRAASVSFIMQSNVLHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            51755..52171
                     /locus_tag="ACHJ12_000852"
     CDS             51755..52171
                     /locus_tag="ACHJ12_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988682.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTTHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            52168..54687
                     /locus_tag="ACHJ12_000853"
     CDS             52168..54687
                     /locus_tag="ACHJ12_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4988683.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RTDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLTRHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRITRVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRTIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFD
                     VECGFSAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTER
                     KQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHE
                     SAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
     gene            54684..55088
                     /locus_tag="ACHJ12_000854"
     CDS             54684..55088
                     /locus_tag="ACHJ12_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988684.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLVTINRKAAIARYVHSAAGRQSRR"
     gene            55098..55307
                     /locus_tag="ACHJ12_000855"
     CDS             55098..55307
                     /locus_tag="ACHJ12_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988685.1"
                     /translation="MNITRNSQLKERTMNSTRRISDRELTKMIESAQQLLSRQNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            55304..55630
                     /locus_tag="ACHJ12_000856"
     CDS             55304..55630
                     /locus_tag="ACHJ12_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4988686.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(55696..55959)
                     /locus_tag="ACHJ12_000857"
     CDS             complement(55696..55959)
                     /locus_tag="ACHJ12_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4988687.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(55997..57049)
                     /locus_tag="ACHJ12_000858"
     CDS             complement(55997..57049)
                     /locus_tag="ACHJ12_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4988688.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLCF
                     DVDAGHVAEEKAVPVG"
     gene            complement(57046..58848)
                     /locus_tag="ACHJ12_000859"
     CDS             complement(57046..58848)
                     /locus_tag="ACHJ12_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO4988689.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WAALLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAATRPAVYDDGVAATSGGGEGKKRRYRKNDVSGLTAEMLDAWARKNLYAYQLHCREN
                     RHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIIDFAQQ
                     FGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMAT
                     HDKYRTTYFSTPSAKTHQAYLVWNGDEWRGDDPARRAVEFPKESAMRVGCECPDGIWR
                     YIIRLEEAVAGGLSARVDIERIRNLYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEVE
                     METWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQG
                     FNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNRL
                     VMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDVT
                     GHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            59015..60064
                     /locus_tag="ACHJ12_000860"
     CDS             59015..60064
                     /locus_tag="ACHJ12_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO4988690.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     MLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAEEVKAAPEDEVKAEAFTAAKA
                     KLEDAIKAFSTITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGTPAESEKPFDFV"
     gene            60076..61104
                     /locus_tag="ACHJ12_000861"
     CDS             60076..61104
                     /locus_tag="ACHJ12_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO4988691.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLSVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAA
                     EEPAPGGA"
     gene            61122..61820
                     /gene="gpM"
                     /locus_tag="ACHJ12_000862"
     CDS             61122..61820
                     /gene="gpM"
                     /locus_tag="ACHJ12_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO4988692.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            61928..62401
                     /locus_tag="ACHJ12_000863"
     CDS             61928..62401
                     /locus_tag="ACHJ12_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4988693.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVAHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            62398..62871
                     /locus_tag="ACHJ12_000864"
     CDS             62398..62871
                     /locus_tag="ACHJ12_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4988694.1"
                     /translation="MNQLESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            62868..63578
                     /locus_tag="ACHJ12_000865"
     CDS             62868..63578
                     /locus_tag="ACHJ12_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988695.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQATPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQSGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITVMEDQIIAGPRQQKARTWTITLPSRVFLGVTNEEFSHILA
                     RQMNAMGYGLDVDAQDIR"
     gene            63590..64735
                     /locus_tag="ACHJ12_000866"
     CDS             63590..64735
                     /locus_tag="ACHJ12_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO4988696.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITARDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            64737..65198
                     /locus_tag="ACHJ12_000867"
     CDS             64737..65198
                     /locus_tag="ACHJ12_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO4988697.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            65203..65508
                     /locus_tag="ACHJ12_000868"
     CDS             65203..65508
                     /locus_tag="ACHJ12_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO4988698.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            65498..66250
                     /locus_tag="ACHJ12_000869"
     CDS             65498..66250
                     /locus_tag="ACHJ12_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4988699.1"
                     /translation="MTHKPYISPFLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYLNDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDAFQAAGFTWRGIVAWNKGRGSRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRSD"
     gene            66250..66588
                     /locus_tag="ACHJ12_000870"
     CDS             66250..66588
                     /locus_tag="ACHJ12_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO4988700.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGMK"
     gene            66585..66971
                     /locus_tag="ACHJ12_000871"
     CDS             66585..66971
                     /locus_tag="ACHJ12_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988701.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDGR"
     gene            67071..67346
                     /locus_tag="ACHJ12_000872"
     CDS             67071..67346
                     /locus_tag="ACHJ12_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO4988702.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQIVEKINEVYSPKLEIELKN"
     gene            complement(67310..67537)
                     /locus_tag="ACHJ12_000873"
     CDS             complement(67310..67537)
                     /locus_tag="ACHJ12_000873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988703.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            67536..69722
                     /locus_tag="ACHJ12_000874"
     CDS             67536..69722
                     /locus_tag="ACHJ12_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4988704.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAAWSLYTLALTKVRSAIIATSLAARLAGVSFLT
                     MSAPVLLVAAAIAALVVVAIKFWQPVKAFIRGFIDGFTSALDAMSPVTAAFGDIARAL
                     APVWQGIKTLFSAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVIS
                     VIRTLIDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            69719..70069
                     /locus_tag="ACHJ12_000875"
     CDS             69719..70069
                     /locus_tag="ACHJ12_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO4988705.1"
                     /translation="MNTPSLYIDMLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            70047..71231
                     /locus_tag="ACHJ12_000876"
     CDS             70047..71231
                     /locus_tag="ACHJ12_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4988706.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLDVTVYIRSSTTLPPDGAEGLKQDISDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            71224..71772
                     /locus_tag="ACHJ12_000877"
     CDS             71224..71772
                     /locus_tag="ACHJ12_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4988707.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTSLYRRRIAHAFVNAQDAGEVAGFIAIFE
                     RLGIGHVELQERKPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            71795..73855
                     /locus_tag="ACHJ12_000878"
     CDS             71795..73855
                     /locus_tag="ACHJ12_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4988708.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFVFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGNAAFFGDGWLVKKGERWSLCKGTGYVEGLRAELTEDIPLTTPDFPSR
                     IWLTVWWTGTLTSEWTVEHDIIMADPAATEEILLEAHRKEAARQYFFALADIDEDGNV
                     TDLRPRGTPEERRWLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGEAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSRHSVLTDTGYGDICLGGCLIEVSGDINGGIIRITTGLE
                     SPNPADMYLRTQFTGVVLNKGKSTIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPDGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFNYGTKTTNSAGPFSATISLRTATASPEGSGDWTA
                     YGPGSGANLIARVSEHTHSVSIGEHTHTLALGSHGHGLTINPTGNAENTVKNIAFNYI
                     VRLA"
     gene            73855..74451
                     /locus_tag="ACHJ12_000879"
     CDS             73855..74451
                     /locus_tag="ACHJ12_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4988709.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKAVWRLESGSALLITEPGPLPENVTEIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLNRTDTGNAPDIVWPESPSPDHHAG"
     gene            74463..75188
                     /locus_tag="ACHJ12_000880"
     CDS             74463..75188
                     /locus_tag="ACHJ12_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988710.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHFSVATIRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     SFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            75160..75702
                     /locus_tag="ACHJ12_000881"
     CDS             75160..75702
                     /locus_tag="ACHJ12_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988711.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARAAPASSRKQTPQTPGAKGKTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            75705..77378
                     /locus_tag="ACHJ12_000882"
     CDS             75705..77378
                     /locus_tag="ACHJ12_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988712.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEETRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTLDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            77795..78085
                     /locus_tag="ACHJ12_000883"
     CDS             77795..78085
                     /locus_tag="ACHJ12_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988713.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSDDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            78242..78382
                     /locus_tag="ACHJ12_000884"
     CDS             78242..78382
                     /locus_tag="ACHJ12_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4988714.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(78497..>79375)
                     /locus_tag="ACHJ12_000885"
     CDS             complement(78497..>79375)
                     /locus_tag="ACHJ12_000885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988715.1"
                     /translation="SRLSALAKENGIDVKSDVGKSLFFLCVGKNKGWRKVQKASDYGV
                     IILTEEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFR
                     RSNALIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGD
                     VFYSDRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGEFSFSRMEPRPV
                     HKLPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCLLQSGCCWVKRR
                     EAGDKKKPEFINLFKK"
BASE COUNT        19277 a        21483 c        21297 g        17318 t
ORIGIN      
        1 gaagggcata tcagcaactc tggtggacaa ccataaattt ctgccatttt gcttttgatg
       61 tttgagctaa accgttccgt cttttcgatc tattttagag aattaatcga gatacctaac
      121 attttactta cttcagcaag cgataattta tgcctgatac gcacggcggc tagccaggaa
      181 atacgctggc ttaacattat attgataatc tcccgctcct ggttttccag atcgatcata
      241 ttcccttcct ttggtatttt aatcagcgag ttaccttacc gcccccgcta tccacgtcga
      301 caactcccgt agctcttttt cctggtccgg gaagtcaacc aacagcgctt cgcactcttg
      361 ttgcagcata tccgcgcggt acaggccgcc ttgcagtcgc ccggcgaggg cttccagcgg
      421 cgcggggttg aggctgtcgg taaacacctg ggcgcgggtg atatggcctt tttcaacgtc
      481 gaaatgcagt tccacgccgc cccagctaaa gcgttcatcc agcagatgcg agaatgcagg
      541 agcctgaccg aagttccatt cccagctact ctgacgggca aaggtttcgg cgaagtttgg
      601 caagtctggc gttttgtccg gggagatgat ttccgcttct acacgctcgc cataatgggc
      661 gaaaaaggcc ttggttatgg cctcgcaaac ctgctcatgg gtgatccccg gcagcagctc
      721 ggtgaggttg gtcacgcggg aacgcactga ggtaatgcct ttcgccgcca gtttcttttt
      781 atccggattg agatagtttg ccaggcggct aaggtcggca ttgagcagca aggtgccgtg
      841 gtggaagcca cgatctttgg tttcgcgata ggccgatcct gagactttgc ggtcaccttc
      901 ggcggttttc accaccagat cgttacgccc ggacgcttcg gcgctgacgc caagcgcgtt
      961 cagcgcattg agcacaatcg acgtggagat ggttttatcg tactccggct tgccagccat
     1021 aaaggtaaag caggtattgc cgagatcgtg gaacaccgcg ccgccaccgc tactgcgccg
     1081 tgccaggcgg acgttatctt cttccatccg ccgggtatta cactctttcc acgggttctg
     1141 cgcgcgacca atcactaccg tgtcggcatt gcgccagaga aacagaacgc gctgcgtggc
     1201 gggcatctgg cgaaaaatac actcctccac cgccaggtta aaccacgggt cgtaagagtc
     1261 agagatgagc aggcgtaatg tggacataac gatttccttt cttgtagtaa tgggtcactc
     1321 ttttttctcg ctttcttcct cttccggcac cggtttgctg gcggttaaca ggaagggcga
     1381 ttgctgccag cgggtacgtt taccctgtag cagcgtgcgg gtcagcacta cgccgattgc
     1441 cagtgaaagc agcaacatca ggcgtaaaat gttagtggtg ttatccacct gttgggcttc
     1501 ggtggcgagc gtatgggtgt cgagcgtcag gcgcagatag ccgagcggtc cgtttttccc
     1561 ggcaattggt tcgacaatct gctggttaaa atagccgccc gcttttttac cgtcgagcgc
     1621 cagccggtcg cgcacttcga cgctttcgcc tgaacgggcg ataagatcgc cttgttcgtc
     1681 atacacaccc gcgtcgagga tgcggctttc atccgttaac tgatcgagga tcgcctgaat
     1741 gcgtttttca tctggtgagt cggtacgcat cagcggtgca acgttcagcg tcacctgacg
     1801 cgccagggtg cgggccagtt cttccagctg tggattacgc tgtcgctggt ggttttgact
     1861 aaaccatgac gccccctgca tcagtgccac taacaaggca agacagaaca ggacaatcac
     1921 tgcccgatgc agccggaatt tcagttttgt gcgagccatc ttccaccctt tgaaaatttg
     1981 agacttaatg ttgccagaag caatggatac aaggtagcct catgcgttat tttccctgct
     2041 tcgaacgatt ttacaggagc cttaatgcct aacattacct ggtgcgacct gcctgaagat
     2101 gtctctttat ggccgggtct gcctctttca ttaagtggtg atgaagtgat gccactggat
     2161 taccacgcag gtcgtagcgg ctggctgctg tatggtcgtg ggctggataa acagcgtctg
     2221 acccaatacc agagcaaact gggtgcggcg atggtgattg ttgccgcctg gtgcgtggaa
     2281 gattatcagg tgattcgtct ggcaggttca ctcaccgcac gggctacgcg cctggcccat
     2341 gaagcgcaac tggatgtcgc cccgctgggg aaaatcccgc acctgcgcac gccgggtttg
     2401 ctggtgatgg acatggattc caccgccatc cagattgaat gtattgatga aattgccaaa
     2461 ctggccggaa cgggcgagat ggtggcggaa gtaaccgaac gggcgatgcg cggcgaactc
     2521 gattttaccg ccagcctgcg cagccgtgtg gcgacgctga aaggcgctga cgccaatatt
     2581 ctgcaacagg tgcgtgaaaa tctgccgctg atgccaggct taacgcaact ggtgctcaag
     2641 ctggaaacgc tgggctggaa agtggcgatt gcctccggcg gctttacttt ctttgctgaa
     2701 tatctgcgcg acaagctgcg cctgaccgcc gtggtagcca atgaactgga gatcatggac
     2761 ggtaaattta ccggcaatgt gatcggcgac atcgtagacg cgcagtacaa agcgaaaact
     2821 ttgactcgcc tcgcgcagga gtatgaaatc ccgctggcgc agaccgtggc gattggcgat
     2881 ggagctaatg acctgccgat gatcaaagca gcagggctgg ggattgccta ccatgccaag
     2941 ccaaaagtga atgaaaaggc ggaagtcacc atccgtcacg ctgacctgat gggggtattc
     3001 tgcatcctct caggcagcct gaatcagaag taattgctcg cccgccatcc tgcgggcggc
     3061 acagcattaa cgaggtacac cgtggcaaaa gctccaaaac gcgcctttgt ttgtaatgaa
     3121 tgcggggccg attatccgcg ctggcagggg cagtgcagtg cctgtcatgc ctggaacacc
     3181 atcaccgagg tgcgtcttgc tgcgtcgcca acggtggcgc gtaacgagcg tctcagcggc
     3241 tatgccggta gcgccggggt ggcaaaagtc cagaaactct ccgatatcag ccttgaagag
     3301 ctgccgcgtt tttccaccgg atttaaagag ttcgaccgcg tactaggcgg cggcgtggtg
     3361 ccaggaagtg ccattctgat tggcggtaac cctggtgcgg ggaaatccac gctgttgctg
     3421 caaacgctgt gcaaactggc ccagcagatg aaaacgctgt atgtcaccgg cgaagagtcg
     3481 ctgcaacagg tggcaatgcg cgctcatcga cttggcctgc cgactgacaa tctcaatatg
     3541 ttgtcggaaa ccagcatcga gcagatctgc ctgattgccg aagaagagca accgaaactg
     3601 atggtaattg actcgatcca ggtgatgcat atggcggatg tacaatcatc gcctggtagc
     3661 gtggcgcagg tgcgtgaaac ggcggcttat ctgacgcgct tcgccaaaac gcgcggtgtg
     3721 gcgattgtca tggttgggca cgtaaccaaa gatggctcgc tggctggccc taaagtgctg
     3781 gaacactgta tcgactgttc ggtgcttctg gatggtgatg ccgactcccg ttttcgcacc
     3841 ttgcgcagcc ataaaaaccg cttcggcgcg gtgaatgagc tgggcgtctt cgcgatgacc
     3901 gaacaggggc tgcgtgaagt cagcaaccct tcggcaattt tcttaagtcg cggagatgaa
     3961 gtgacctccg gcagctccgt gatggtggtg tgggaaggaa cgcgtccgtt gctggtggag
     4021 attcaggcgc tggtcgatca ctcgatgatg gcgaatccgc gccgcgtggc agtggggctg
     4081 gaacaaaacc gtctggcaat cctgctggct gtcttgcacc gtcacggtgg tctgcaaatg
     4141 gccgatcagg atgtatttgt gaacgtggtc ggcggcgtga aggtaaccga aaccagtgcc
     4201 gatttagcgt tactgctggc gatggtttcc agcctgcgtg acagaccgct gccacaggat
     4261 ctggtggtgt ttggtgaagt cgggctggca ggggagatcc gcccggtgcc cagcggtcag
     4321 gaacgaattt cagaagcggc gaaacacggt tttcgccggg cgattgttcc ggcggctaac
     4381 gtaccgaaaa aagcgccgga agggatgcag atttttggcg ttaaaaaact ctccgacgcg
     4441 cttagcgtgt tcgacgactt ataatgagag atacggaggg agatatgtcg tcatttgatt
     4501 acctgaaaac tgccatcaag caacagggct gcacgctaca gcaggtagct gatgccagcg
     4561 gtatgaccaa agggtattta agccagttac tgaatgccaa aattaaaagc cccagcgcgc
     4621 aaaagctgga ggcgttgcac cgttttttgg ggcttgaatt tccccgacag aagaaaacca
     4681 ttggtgtcgt attcggtaag ttctacccgc tgcataccgg acatatctac cttatccagc
     4741 gcgcctgtag ccaggttgac gagctgcata tcattatggg ttttgacgat acccgcgacc
     4801 gcgcgttgtt tgaagacagc gccatgtcgc agcagcccac ggtgccggat cgtctgcgct
     4861 ggttattaca aacttttaaa tatcagaaaa atattcgcat tcatgctttc aacgaagagg
     4921 gcatggagcc gtatccgcac ggctgggatg tgtggagcaa cggcatcaaa aagtttatgg
     4981 ctgaaaaggg gattcagccg gacctgatct acacctcgga agaagccgat gcgccacagt
     5041 atatggaaca tctggggatc gatacggtgc tggtcgatcc gaaacgtacc tttatgagta
     5101 tcagcggtgc gcagatccgc gaaaatccgt tccgctactg ggaatatatt cctacggaag
     5161 tgaagccgtt ctttgtacgt accgtggcga tccttggtgg cgagtcgagc ggtaaatcca
     5221 ccctggtaaa caaacttgcc aatatcttca ataccaccag tgcgtgggaa tatggtcgcg
     5281 attatgtctt ttcacacctc ggcggtgatg agatcgcatt gcagtattcc gattacgata
     5341 aaatcgcgct gggccacgca caatacattg attttgcagt gaaatatgcc aataaagtgg
     5401 cgtttattga cactgatttt gtcactaccc aggcgttctg caaaaagtac gaagggcgtg
     5461 agcatccgtt cgtacaggcg ttgattgatg aataccgttt cgatctggtg atcctgctgg
     5521 agaacaacac gccgtgggtg gcggatggtt tacgcagcct cggcagttcg gtggatcgca
     5581 aagagttcca gaacttgctg gtggagatgc tggaagagaa caatatcgaa ttcgtgcggg
     5641 ttgaagagga cgattatgac agccgtttcc tgcgctgcgt ggagttggtg cgggagatga
     5701 tgggggagca gagataaccg cgatgaaacg gctcaaaggc gaggtataaa ataagttttt
     5761 tgatgagttg aaaacctctc tggaagaggc tgtcgagatt aaacaaggtt taaaagaatc
     5821 agcacgggtg acccgctacg aatttgcgga tactaaggct gttgtaggcc ggaaaaggcg
     5881 ttcacgccgt atccggcatt cggtgctcaa tgcctgatgc gacgcttacc gcgtcttatc
     5941 aggcctggag attcacaacg tattgaaatt tcttggtttg taggccggat aaggcgttca
     6001 cgccgcatcc ggcattttac gcattacttc gcaatacgct tgtacttgat acgcttcggc
     6061 tccagtgcgt ctgcgcccag cgtgcgtttc ttgtactctt cgtactcagt aaagttacct
     6121 tcgaagaact caactttacc ttcatcctgg tagtccagga tgtgcgtggc gatacggtcg
     6181 aggaaccaac ggtcgtgcga gataaccatc gcacagcccg ggaactccag cagggcgttt
     6241 tccagcgcgc gcagggtttc gatatccagg tcgttggttg gttcgtcgag cagcagcatg
     6301 ttgccgccaa cctgcagcag cttcgccaga tgcagacgac cgcgctcacc accggaaagt
     6361 tcaccaacgc gtttaccctg atcaacccct ttaaagttaa agcggccaac gtaggcgcgg
     6421 cttggcatct cggtgttgcc gatcttcata atatccagcc cgccggaaac ttcttcccaa
     6481 acggttttgc tgttatccat tgagtcacgg aactgatcaa ccgacgccag tttcaccgtt
     6541 tcacctaaag tgatggtgcc gctgtccggc tgttcctgac cggagatcat acggaacagg
     6601 gtcgatttac ccgcgccgtt cggaccgatg atcccgacga tcgccccttt cgggatcgag
     6661 aagctcaggt catcaatcag caggcgatcg ccgtaggatt tacgcaggtt gctgacttcc
     6721 agcactttat cgcccagacg cggtccaggt ggaataaaca gttcgttggt ttcgttacgt
     6781 ttctgatatt cggtgctgtt cagctcttca aagcgtgcca gacgtgcttt acctttcgac
     6841 tggcggcctt tagttccctg acgtacccac tccagctctt tctcgatcga cttacgacgc
     6901 gccgcttctt gtgaagcttc ctgcgccagg cgctgatctt tctgctccag ccaggaggag
     6961 tagttgcctt cccacggaat accttcaccg cggtcaagtt cgaggatcca gcctgcaacg
     7021 ttatcgagga agtaacggtc gtgagtaatc gccacaacgg tgccttcgaa gtcgtgcagg
     7081 aagcgttcca gccaggccac ggattcggca tccaggtggt tggtcggttc gtcgagcagc
     7141 agcatgtctg gtttttccag aagcaggcgg cacaacgcca cgcgacgacg ctcaccaccg
     7201 gagaggttag cgattttcgc gtcccaatct ggcagacgca gcgcatccgc cgcacgctcc
     7261 agctgtacgt tcaggttatg accgtcgtga gcctgaatga tctcttccag acggccttgt
     7321 tcagcggcca gcttgtcaaa atcggcatcc ggatcggcgt acagcgcata cacttcatcc
     7381 aggcgtttca gggcgttaac cacttcagaa accgcttctt caatggactc acgcacggtg
     7441 tgttccgggt tcagctgcgg ttcctgcggc aggtaaccaa tcttgatgtc tggctgcgga
     7501 cgcgcttcac cttcgatgtc tttatcaatg cccgccataa tgcgcagcag ggtagactta
     7561 cccgcgccgt tcagacccag gacaccaatt tttgccccag ggaagaaact cagagagatg
     7621 tttttcaaaa tatgacgttt cggcggaaca actttgccga cacgatgcat ggtataaacg
     7681 aattgagcca cgttggactt cgcctctatg tttatcgtga tgatgagttt tcaaaggcga
     7741 agtgtagcct ttttccccgc ctaatcccag ttggtcgatc accttcgcag taaaagtaaa
     7801 aaagtgtccg taacgtggcg taaacggcaa tgactggtta gcataaatct attacgcggc
     7861 atgacgctgc attgatgtat ttacacttag aggatgcgct tgtggaaaaa gccaaacaag
     7921 ttacctggcg gctgttggct gccggtgtct gtctgctgac ggtcagcagc gtggcgcgag
     7981 ccgactcact ggatgagcag cgtagccgtt acgcgcaaat caagcaggcc tgggataatc
     8041 gacaaatgga tgtggtcgaa caaatgatgc ctggactgaa ggattatccg ctttatccct
     8101 acctggaata ccgccagatc accgatgatc tgatgaatca accggcggtg acggtcacta
     8161 actttgttcg cgctaacccc acgcttcctc ccgctcgcac gctgcaatct cgtttcgtca
     8221 atgaactggc gcggcgtgaa gactggcgtg gcttgttagc ctttagcccg gaaaagcccg
     8281 gaactaccga agcgcaatgt aattactact atgcgaaatg gaacaccggg cagagtgaag
     8341 aagcctggca aggggcgaaa gagctgtggc taaccggcaa gagccagcct aacgcctgtg
     8401 acaagttatt tagcgtctgg cgtgcgtcag gtaaacaaga tccgctggcg tatttagagc
     8461 gtatccgtct ggcgatgaaa gcgggtaaca ctggcctggt aacagtgctg gcagggcaga
     8521 tgcctgccga ttaccagact atcgcctcgg caatcatttc actggcgaac aaccctaata
     8581 cggtactgac cttcgtgcgt acaaccggag cgaccgattt tacccgtcaa atggcagcgg
     8641 tggcgtttgc cagtgtggcg cggcaggatg ctgaaaatgc acggctgatg atcccgtcgc
     8701 ttgcccaggc gcagcagctt aatgaagatc agattcagga gctgcgcgat atcgtcgcct
     8761 ggcgtttgat gggcaacgat gtcaccgaca agcaggcgaa atggcgcgat gacgccatta
     8821 tgcgctcgca atccacgtcg ttgattgaac gccgtgtgcg aatggcactt ggcaccggcg
     8881 atcgtcgcgg cctgaatacc tggctggcgc gtctgccgat ggaagcaaaa gagaaagatg
     8941 aatggcgtta ctggcaggcg gatttattgc tggaacgcgg acgtgaagct gaagcgaaag
     9001 agattttgca tcaactcatg caacagcgtg gtttctaccc gatggttgca gcacaacgca
     9061 tcggagaaga gtatgagctg aagattgata aagcgccgca gaatgttgac agcgccctga
     9121 ctcaggggcc ggagatggcg cgcgtgcgcg agttgatgta ctggaatctc gataacaccg
     9181 cgcgtagcga gtgggccaat ctggtgaaga gcaagtcaaa aacagagcag gctcaactgg
     9241 cgcggtatgc tttcaacaac caatggtggg atcttagcgt tcaggcaacg atcgccggga
     9301 agctatggga tcatctggaa gagcgattcc cgctggctta caacgatctt ttcaaacgct
     9361 acaccagcgg taaggagatc ccgcaaagct atgcgatggc gattgcccgt caggagagcg
     9421 cctggaatcc aaaagtgaaa tcaccggtag gggccagcgg cctgatgcag attatgcctg
     9481 gtacagcgac ccatacggtg aagatgttct ctattcccgg ttatagtagc cctgggcaat
     9541 tgctggatcc ggaaacgaat atcaacattg gcaccagtta tctgcaatat gtttatcagc
     9601 agtttggcaa taaccgtatt ttctcctcag cagcttataa cgccggacca gggcgggtgc
     9661 gaacctggct tggcaacagc gccgggcgta tcgacgctgt ggcatttgtc gagagtattc
     9721 cgttctccga gacgcgcggt tatgtgaaga acgtgctggc ttatgacgct tactaccgct
     9781 atttcatggg ggataaaccg acgttgatga gcgccacgga atggggacgt cgttactgat
     9841 ccgcacgttt atgatatgct atcgtactct ttagcgagta caaccggggg aggcattttg
     9901 cttcccccgc taacaatggc gacatattat ggcccaacaa tcaccctatt cagcagcgat
     9961 ggcagaacag cgtcaccagg agtggttacg ttttgtcgac ctgcttaaga atgcctacca
    10021 aaacgatctc catttaccgt tgttaaatct gatgctgacg ccagatgagc gcgaagcgtt
    10081 ggggactcgc gtgcgtattg tcgaagagct gttgcgcggc gaaatgagcc agcgtgagtt
    10141 aaaaaatgaa ctcggcgcgg gcatcgcgac gattacgcgt ggatctaaca gcctgaaagc
    10201 cgcgcccgtg gagctgcgcc agtggctgga agaggtgttg ctgaaaagcg attgattttg
    10261 tagatactga taagacgcgg cagcgtcgca tcaggcatcg tgcaccgaat gccggatgcg
    10321 gcgtgaacgc cttatccgtc ctacaatgcc tgatacacgg cattgtgaaa cggactcagc
    10381 gccaggatca ccgcctggtg atagacgctg gcgcgagtga gtttcccggc ggtaaacacg
    10441 ccgatcgccc cttccttacg accaatctca tcaataccgg tataacgcga catcacggga
    10501 ccaagcgcct caccttcacg cactttctgc agaatcaccg caggtaacgg caaagtagcc
    10561 gaacgcgcct cgccgcgctg gctggtgttt tcaatcacca cccaactgaa agtgctgtca
    10621 ccatcgatgc cagcttcaat cgccacccaa aaatcagcct ctggaagtaa acggcgggca
    10681 tttgctaccc gatttcgtgc gccagcgcgc gtttcctcac tgccaaaggg ttgttccggt
    10741 acaccgctct cgacggcaac ggatgcaata tggcaggatc cttcgccgaa gatctcgtga
    10801 aatgcctgca gaatggcctg aattttagcg ggattggtgg tcgcacagac aacttggtgc
    10861 ataatcagca ttactcagaa aattaacgtt acagcagtat acggaaaaaa agcatgttac
    10921 aggtatacct agtccgccac ggtgaaacgc agtggaacgc cgagcgacgt attcagggcc
    10981 agtctgacag cccgctgacc gccaaaggtg agcaacaggc gatgcaggtg gcaacccgtg
    11041 ccaaagagct tggcattacg catatcatca gtagcgattt aggacgcacc cggcgtacgg
    11101 cggaaatcat cgcccaggcc tgcggctgtg acatcatctt tgattctcgc ctgcgtgaat
    11161 taaacatggg tgtgctggaa aaaagacata tcgattctct gaccgaagaa gaagagaact
    11221 ggcgtcggca gctggtcaat ggcaccgttg acgggcgtat tcctgaaggc gagtcaatgc
    11281 aggagctcag cgatcgcgtc aatgcggcac tggaatcttg ccgggactta ccgcagggaa
    11341 gccgaccgct gctggtgagc catggtattg cactgggatg tctggtgagt acgattctcg
    11401 gattaccagc atgggcagag cgccgcttac gtctgcgtaa ctgttctatt tcgcgcgtgg
    11461 attatcagga aagcctgtgg ctggcgtctg gctgggttgt tgaaaccgca ggggacatct
    11521 cacatctgga cgcccctgca ttagatgagt tgcagcgtta acgacggatc ggaatcagca
    11581 gttcacagcg cagattaatt gggcgatctc ccgctttggc atcttctgcc gggtagtatc
    11641 gctcaatatc ctgaccttta cggcgcgtca gattgagcat tggcatgcac gttccgtata
    11701 ccgtcaggat aaactcctgc acgccggttc ccagaccttc ataggtaaac atcacatatt
    11761 cgccgccctg cagcatcacc ggatgccccg tcagtacata gccatctgcc tgatcctggg
    11821 ctaacgcggt ggtatagaat acttcttgtt cgtcgtcttt atcctgactc ggacgcgttt
    11881 cattcaggcc gtagagcact ggcggaatgg tcggcgcgtt gccgagaaaa tcgtgccaga
    11941 actgataacg catttcatgg cggaaatcag agatttgctc cagcgaacag gagtagctct
    12001 gggtaacacc aatcagcggc gtatcttcca gggtgacaaa tttgtgctct ggcatagtga
    12061 attcacccag acgtagcggc gggcgaatac caaaggcgct ccattcagga gaacggcggt
    12121 aaagtgcagg agtctgggca aactgcttct tgaatgcgcg ggtaaatgtc tgttgagagt
    12181 cgaagcggta ttgcagcgcg atgtccagaa tcggacgcgc agtcaggcgt agtgcgaccg
    12241 ccgatttcga caaacgacga gcacgaatat acgcgccaat agcatggcca gtgacatctt
    12301 taaacattct ctgtaagtgc cacttggaat aacctgcttt cgccgctaca ttgtcgagcg
    12361 acaggggctg atccagatga ccttccagcc agattaaaag gtcgcgaata atgccggcct
    12421 gatccataaa atatcctcat cctttcaaca acgagcacct gacatcaggt aattggataa
    12481 tagcattttt tgctgtttta gcattcagtg tttttttctt agtagagtat gttttagggc
    12541 ttgacggaaa taaaagtatt gagattttgt tcttaatcaa tatgttattt accgtgacga
    12601 actaattgct cgtgtaatag ataaaaatgg taacaatatg aaatacaagc atttgatcct
    12661 gtctttaagc ctgataatgc tggggccatt ggctcatgca gaagagattg gttcggtcga
    12721 caccgtattt aaaatgatcg gcccggatca caaaattgtt gtggaagcct ttgacgatcc
    12781 cgatgtgaaa aatgtcacct gttatgtgag ccgggcgaaa accgggggta ttaaaggggg
    12841 attgggcctg gcggaagata cctccgatgc ggccatttct tgtcagcaag tcgggccgat
    12901 tgaactgtcg gatcgtatta aaaacggcaa agctcagggc gaggtagtat tcaaaaaacg
    12961 tacgtccctg gtctttaagt cgttacaggt cgtgcgcttt tatgatgcta aacgcaacgc
    13021 gctcgcttat ctggcttact ccgacaaagt tgtagaaggt tcgccgaaaa acgcgattag
    13081 cgcggttcct gtcatgccgt ggcggcaata acagaggcga tttatgcaac gggaaacggt
    13141 ctggttagtg gaagatgagc aagggatagc cgacacgctg gtctacatgt tgcagcagga
    13201 aggttttgcc gtcgaggtct ttgagcgagg cttgccggtg ctggataaag ctcgccagct
    13261 ggttcccgac gtcatgattc tcgacgttgg tttgcctgat atcagcggct ttgaattgtg
    13321 ccgccagtta ctggcgctcc atccggcgtt acctgtactg ttcctgacgg cccgaagtga
    13381 agaggtcgat cgcctgcttg ggctggaaat tggcgctgac gactatgtgg ctaaaccgtt
    13441 ttcaccccgc gaagtgtgcg ccagggtgcg caccttactg cgtcgggtga agaagttctc
    13501 gtcgccgtct cccgtcatcc gtattggtca ttttgaattg aatgaacccg cggcgcagat
    13561 cagctggttt gacacgccat taacgctgac gcggtatgag tttttattgc tgaagacgtt
    13621 actcaagtcg ccaggccgcg tctggtcccg ccagcaactg atggatagcg tgtgggaaga
    13681 tgcgcaggac acctatgatc gcaccgttga tacccacatt aaaacgttgc gtgccaagct
    13741 gcgcgccatc aatcccgatc tttcaccgat taatactcat cgcggcatgg gatatagcct
    13801 gaggggcctg taatgcgtat cggcatgcgg ttgctgctgg gctatttttt actggtggcg
    13861 gtggcggcct ggttcgtact ggctattttt gtcaaagaag ttaaaccggg cgtgcgaaga
    13921 gcaacggagg ggacgttgat cgacaccgca acgttgctgg cggagctggc gcgtcccgat
    13981 ttgctctctg gggacccaac gcatgggcaa ctggcgcagg cgtttaatca gctacaacat
    14041 cgcccgtttc gcgccaatat cggtggcatt aacaaagtgc gcaatgaata tcatgtctat
    14101 atgaccgatt cgcagggtaa agtattgttc gattcggcaa ataaagccgt tgggcaggat
    14161 tattcgcgct ggaatgacgt ctggctaacg ttgcgtggtc agtatggtgc gcgcagcacg
    14221 ttgcaaaatc ctgccgatcc cgaaagttcg gtgatgtatg ttgccgcgcc gattatggac
    14281 ggctcgcggc ttattggcgt tttgagcgta ggcaaaccga acgcggcgat ggctccggtc
    14341 attaaacgca gcgagcggcg aattttatgg gccagcgcca ttttgttggg gatcgcactg
    14401 gtgattggcg caggcatggt ttggtggatc aaccgctcca ttgcccggct cactcgctat
    14461 gctgattccg tcactgacaa taagcccgtt cctcttcccg atctcggtag tagcgagctg
    14521 cgtaaactcg cgcaggcgct ggaaagtatg cgcgtgaagc tggaagggaa aaactatatt
    14581 gagcagtatg tttatgcgtt aacccatgag ctaaaaagcc cactggcggc gattcgtggc
    14641 gcggcggaaa ttttacgcga aggtccgccg cctgaagtag tggctcgttt tactgacaac
    14701 attctgacgc aaaatgcgcg aatgcaggca ctggtggaaa cgttactacg ccaggcaaga
    14761 ctggagaatc gtcaggaagt cgttttgact gctgttgatg tggcggcatt attccgccgc
    14821 gtcagcgaag cgcgcaccgt gcagttggca gaaaaaaaca tcactctaca tgttacgccc
    14881 accgaggtta atgttgctgc tgaaccggcg ttactggatc aggcgctggg gaatttactg
    14941 gataacgcca tcgattttac ccccgagagc ggtcgtataa cgctaagcgc cgaagtggat
    15001 caggaacacg tcacgcttaa ggtgctggat accggtagtg gtattcctga ctacgcgctg
    15061 tcacgtattt ttgaacgctt ttactctttg ccgcgtgcaa atgggcaaaa aagcagcggt
    15121 ctggggttgg cgttcgtcag tgaggtcgcc cgtttgttta acggcgaagt cacgctgcgc
    15181 aacgtgcagg aaggtggcgt gctggcctcg cttcgacttc accgtcactt cacatagctt
    15241 caaattcttc ccacatagcc ttcgtatcct gctgccattg caaaggagaa gactatgttg
    15301 aaatcccccc tgttctggaa aatgaccacc ctgtttggtg cagtattgct gttgttgatt
    15361 ccgataatgc tgattcggca ggtgattgtc gaacgtgccg attaccgtag cgatgtggaa
    15421 gatgcgattc gccaaagtac cagcgggccg caaaaactcg ttgggccgct catcgctatt
    15481 cctgtgaccg agctttatac ggtgcaggaa gacgataaaa ccgtggagcg gaaacgaagt
    15541 tttatccatt tttggttacc tgagtcattg atggttgatg gcaatcagaa cgttgaagaa
    15601 cgcaagatag ggatttatac cggtcaggtc tggcacagtg atttaacgct aaaagccgat
    15661 ttcgatgttt cgcgtcttag cgaactcgac gcaccaaata tcaccttagg caagccattt
    15721 attgtgatta gcgtcgggga tgcgcgtggt attggtgtgg tgaaagcgcc tgaagttaac
    15781 ggaacagcgc tgaccatcga acccggtacc gggttagagc aaggcgggca gggcgtgcat
    15841 atccctttac ccgaagggga ctggcgcaag cagaacctga agctgaatat ggccctgaat
    15901 ttaagcggta ccggcgatct ttctgtggtg cctgccgggc gtaatagcga aatgacctta
    15961 accagcaact ggccgcatcc cagtttttta ggtgattttt tgccagccaa acgggaagtt
    16021 agcgagtcag gttttcaggc gcaatggcaa agcagctggt ttgcaaataa tctcggtgag
    16081 cgttttgctt caggcaatga taccggctgg gaaaacttcc ccgcgtttag cgtcgcagtg
    16141 acgacgccag ccgatcaata ccaattaact gaccgggcga ctaagtatgc cattctgctg
    16201 attgcgctga cttttatggc gttctttgtt tttgaaacgc tcaccgcgca acgcttacac
    16261 ccaatgcaat atttgctggt ggggctttca ttggtgatgt tttatttgct cttgctggcg
    16321 ctttctgaac ataccggttt taccgtggca tggataatcg ccagtctgat tggggcgtta
    16381 atgaacggta tttatttgca ggcggtattg aaaggctggc gcaacagcat gttgtttacc
    16441 ctcgcgctgt tgttgctgga tggtgtgatg tggggactgc tcaactctgc cgatagcgcg
    16501 ctgttgttgg gaaccagtgt gctggtggtg gcgctggccg gcatgatgtt tgtgacccgt
    16561 aatatcgact ggtatgcgtt ttcactgccg aaaatgaaag ccagtaaaga agttacaatg
    16621 gacgatcaat tacgtatctg gaaataaggt tgaaaaataa aaacggcgct aaaaagcgcc
    16681 gttttttttg acggtggtaa agccgattaa tcttccagat caccacagaa gcgataacct
    16741 tcaccgtgga tggtggcgat gatttccggc gtatccggcg tagattcgaa atgtttacga
    16801 atacggcgga tcgtcacgtc tacagtacgg tcgtgtggtt tcagctcacg gccggtcatt
    16861 ttcttcagca gttcagcacg agactgaatt ttgcctgggt tttcacagaa gtgaagcatg
    16921 gcgcggaact cgctgcgcgg cagcttgtac tgctcgccat cagggccgat caacgaacgg
    16981 ctgttgatgt ccagttccca accattgaac ttgtagcttt caacgctacg acgttcttcg
    17041 ctgacagtac ccagattcat ggtacgggac agcaggttgc gtgcacgaat cgtcagttca
    17101 cgcgggttga acggtttggt gatgtagtca tctgcaccga tttcgaggcc gagaatttta
    17161 tcgacttcgt tgtcacggcc agtcaggaac atcaacgcaa cattcgcctg ctcgcgcagt
    17221 tcacgcgcta acagaagacc gttcttaccc ggcagattga tatccatgat caccaggttg
    17281 atgtcatatt cagagaggat ctgatgcatt tccgcgccat ctgtcgcttc gaaaacatca
    17341 tagccttccg cttcgaaaat acttttcaac gtgttgcgtg ttaccaactc gtcttcaacg
    17401 ataagaatgt gcggggtctg catgtttgct acctaaattg ccaactaaat cgaaacagga
    17461 agtacaaaag tccctgacct gcctgatgca tgctgcaaat taacatgatc ggcgtaacat
    17521 gactaaagta cgtaattgcg ttcttgatgc actttccatc aacgtcaaca acatcattag
    17581 cttggtcgtg ggtactttcc ctcaggaccc gacagtgtca aaaacggctg tcatcctaac
    17641 cattttaaca gcaacataac aggctaagag gggccggaca cccaataaaa ctacgcttcg
    17701 ttgacatata tcaagttcaa ttgtagcacg ttaacagttt gatgaaatca tcgtatctaa
    17761 atgctagctt tcgtcacatt attttaataa tccaactagt tgcatcatac aactaataaa
    17821 cgtggtgaat ccaattgtcg agatttattt tttataaaat tatcctaagt aaacagaagg
    17881 atatgtagca ttttttaaca attcaaccgt tagtacagtc aggaaatagt ttagcctttt
    17941 ttaagctaag taaagggctt tttctgcgac ttacgttaag aatttgtaaa ttcgcaccgc
    18001 gtaataagtt gacagtgatc accctgttcg cggttatttg gtcaagaaga gtggcaatat
    18061 gcgtataacg attattctgg tcgcacccgc cagagcagaa aatattgggg cagcggcgcg
    18121 ggcaatgaaa acgatgggtt ttagcgaact gcggattgtc gatagtcagg cacatctgga
    18181 gccagccacc cgctgggtcg cacatggatc tggtgatatt attgataata ttaaagtttt
    18241 cccgacactg gttgaatcgt tacacgatgt cgatttcact gtcgccacca ctgcgcgcag
    18301 tcgggcgaaa tatcattact acgccacgcc agtagaactg gtgccgctgt tagaggaaaa
    18361 atcttcatgg atgagccatg ccgcgctggt gtttggtcgc gaagattccg ggctgactaa
    18421 cgaagagtta gcgttggctg acgttcttac tggtgtgccg atggtggcgg attatccttc
    18481 gctcaatctg gggcaggcgg tgatggtcta ttgctatcaa ttagcaacat taatacaaca
    18541 accggcgaaa agtgacacaa cggcagacca acatcaactg caagctttac gcgaacgagt
    18601 catggcattg ctgacgactc tggcagtggc agatgacata aaactggtcg actggttaca
    18661 acaacgcctg gggcttttag agcaacgaga cacggcaatg ttgcaccgtt tgctgcatga
    18721 tattgaaaaa aatatcacca aataaaaaac gccttagtaa gtatttttca gcttttcatt
    18781 ctgactgcaa cgggcaatat gtctctgtgt ggattaaaaa aagagtgtct gatagcagct
    18841 tctgaactgg ttacctgccg tgagtaaatt aaaattttat tgacttaggt cactaaatac
    18901 tttaaccaat ataggcatag cgcacagaca gataaaaatt acagagtaca caacatccat
    18961 gaaacgcatt agcaccacca ttaccaccac catcaccatt accacaggta acggtgcggg
    19021 ctgacgcgta caggaaacac agaaaaaagc ccgcacctga cagtgcgggc ttttttttcg
    19081 accaaaggta acgaggtaac aaccatgcga gtgttgaagt tcggcggtac atcagtggca
    19141 aatgcagaac gttttctgcg tgttgccgat attctggaaa gcaatgccag gcaggggcag
    19201 gtggccaccg tcctctctgc ccccgccaaa atcaccaacc acctggtggc gatgattgaa
    19261 aaaaccatta gcggccagga tgctttaccc aatatcagcg atgccgaacg tatttttgcc
    19321 gaacttttga cgggactcgc cgccgcccag ccggggttcc cgctggcgca attgaaaact
    19381 ttcgtcgatc aggaatttgc ccaaataaaa catgtcctgc atggcattag tttgttgggg
    19441 cagtgcccgg atagcatcaa cgctgcgctg atttgccgtg gcgagaaaat gtcgatcgcc
    19501 attatggccg gcgtgttaga agcgcgtggt cacaacgtta ccgttatcga tccggtcgaa
    19561 aaactgctgg cagtggggca ttacctcgaa tctaccgtcg atattgctga gtccacccgc
    19621 cgtattgcgg caagccgcat tccggctgat cacatggtgt tgatggcagg tttcaccgcc
    19681 ggtaatgaaa aaggcgaact ggtggtactt ggacgcaacg gttccgacta ctccgcggcg
    19741 gtgctggctg cctgtttacg cgccgattgt tgcgagattt ggacggacgt tgacggggtc
    19801 tatacctgcg acccgcgtca ggtgcccgat gcgaggttgt tgaagtcgat gtcctaccag
    19861 gaagcgatgg agctttccta cttcggcgct aaagttcttc acccccgcac cattaccccc
    19921 atcgcccagt tccagatccc ttgcctgatt aaaaataccg gaaatcctca agcaccaggt
    19981 acgctcattg gtgccagtcg tgatgaagac gaattaccgg tcaagggcat ttccaatctg
    20041 aataacatgg caatgttcag cgtttccggc ccggggatga aaggaatggt cggcatggcg
    20101 gcgcgcgtct ttgccgcgat gtcacgcgcc cgtatttccg tggtgctgat tacgcaatca
    20161 tcttccgaat acagtatcag tttctgcgtt ccacaaagcg actgtgtgcg agctgaacgg
    20221 gcaatgcagg aagagttcta cctggaactg aaagaaggct tactggagcc gctggcggtg
    20281 acggaacggc tggccattat ctcggtggta ggtgatggta tgcgcacctt gcgtgggatc
    20341 tcggcgaaat tctttgccgc gctggcccgc gccaatatca acattgtcgc cattgctcag
    20401 ggatcttctg aacgctcaat ctctgtcgtg gtaaataacg atgatgcgac cactggcgtg
    20461 cgcgttactc atcagatgct gttcaatacc gatcaggtta tcgaagtgtt tgtgattggc
    20521 gtcggtggcg ttggcggtgc gctgctggag caactgaagc gtcaacaaag ctggctgaag
    20581 aataaacata tcgacttacg tgtctgcggt gttgccaact cgaaggcact gctcaccaat
    20641 gtgcatggcc taaatctgga aaactggcag gaagaactgg cgcaagccaa agagccgttt
    20701 aatctcgggc gcttaattcg cctcgtgaaa gaatatcatc tgctgaaccc ggtcattgtt
    20761 gactgcacct ccagccaggc agtggcggat caatatgccg acttcttgcg cgaaggtttc
    20821 cacgttgtca cgccgaacaa aaaggccaac acctcgtcga tggattacta ccatctgttg
    20881 cgtcatgcgg cggaaaaatc gcggcgtaaa ttcctctatg acaccaacgt tggggctgga
    20941 ttaccggtta ttgagaacct gcaaaatctg ctcaatgctg gtgatgaatt gatgaagttc
    21001 tccggcattc tttcaggttc gctttcttat atcttcggca agttagacga aggcatgagt
    21061 ttctccgagg cgactactct ggcgcgggaa atgggttata ccgaaccgga tccgcgagat
    21121 gatctttctg gtatggatgt agcgcgtaag ctattgattc tcgctcgtga aacgggacgt
    21181 gaactggagc tggcggatat tgaaattgaa cctgtgctgc ccgcagagtt taacgctgag
    21241 ggtgatgttg ccgcttttat ggcgaatctg tcacagctcg acgatctctt tgccgcgcgc
    21301 gtggcgaagg cccgtgatga aggaaaagtt ttgcgctatg ttggcaatat tgatgaagat
    21361 ggtgcctgcc gcgtgaagat tgccgaagtg gatggtaatg atccgctgtt caaagtgaaa
    21421 aatggcgaaa acgccctggc cttttatagc cactattatc agccgctgcc gttggtgctg
    21481 cgcggatatg gtgcgggcaa tgacgttaca gctgccggtg tctttgccga tctgctacgt
    21541 accctctcat ggaagttagg agtctgacat ggttaaagtt tatgccccgg cttccagtgc
    21601 caatatgagc gtcgggtttg atgtgctcgg ggcggcggtg acacctgttg atggtgcatt
    21661 gctcggagat gtagtcacgg ttgaggcggc agagacattc agtctcaaca acctcggacg
    21721 ctttgccgat aagctgccgt cagaaccacg ggaaaatatc gtttatcagt gctgggagcg
    21781 tttttgccag gagcttggca agcaaattcc agtggcgatg actctggaaa agaatatgcc
    21841 aatcggttcg ggcttaggct ccagcgcctg ttcggtggtc gcggcgctga tggcgatgaa
    21901 tgaacactgt ggcaagccgc ttaatgacac tcgtttgctg gctttgatgg gcgagctgga
    21961 aggacgaatc tccggcagca ttcattacga caacgtggca ccgtgttttc ttggtggtat
    22021 gcagttgatg atcgaagaaa acgacatcat cagccagcaa gtgccagggt ttgatgagtg
    22081 gctgtgggtg ctggcgtatc cggggattaa agtctcgacg gcagaagcca gggctatttt
    22141 accggcgcag tatcgccgcc aggattgcat tgcgcacggg cgacatctgg ctggcttcat
    22201 tcacgcctgc tattcccgtc agcctgagct tgccgcgaag ctgatgaaag atgttatcgc
    22261 tgaaccctac cgtgaacggt tactgcctgg cttccggcag gcgcggcagg cggtcgcgga
    22321 aatcggcgcg gtagcgagcg gtatctccgg ctccggcccg accttgttcg ctctatgtga
    22381 caagccggat accgcccagc gcgttgccga ctggttgggt aagaactacc tgcaaaatca
    22441 ggaaggtttt gttcatattt gccggctgga tacggcgggc gcacgagtac tggaaaacta
    22501 aatgaaactc tacaatctga aagatcacaa cgagcaggtc agctttgcgc aagccgtaac
    22561 ccaggggttg ggcaaaaatc aggggctgtt ttttccgcac gacctgccgg aattcagcct
    22621 gactgaaatt gatgagatgc tgaagctgga ttttgtcacc cgcagtgcga agatcctctc
    22681 ggcgtttatt ggtgatgaaa tcccgcagga aatcctggaa gagcgcgtgc gcgcggcgtt
    22741 tgccttcccg gctccggtcg ccaatgttga aagcgatgtc ggttgtctgg aattgttcca
    22801 cgggccaacg ctggcattta aagatttcgg cggtcgcttt atggcacaaa tgctgaccca
    22861 tattgcgggc gataagccag tgaccattct gaccgcgacc tccggtgata ccggagcggc
    22921 agtggctcat gctttctacg gtttaccgaa tgtgaaagtg gttatcctct atccacgagg
    22981 caaaatcagt ccactgcaag aaaaactgtt ctgtacgttg ggcggcaata tcgaaactgt
    23041 tgccatcgac ggcgatttcg atgcctgtca ggcgctggtg aagcaggcgt ttgatgatga
    23101 agaactgaaa gtggcgctgg ggttaaactc agctaactcg attaacatca gccgtttgct
    23161 ggcgcagatt tgctactact ttgaagctgt tgcgcagctg ccgcaggaag cgcgcaacca
    23221 gctggttgtc tcggtgccaa gcggaaactt cggcgatttg acggcgggtc tgctggcgaa
    23281 gtcactcggt ctgccggtga aacgttttat tgctgcgacc aacgtgaacg ataccgtgcc
    23341 acgtttcctg cacgacggtc agtggtcacc caaagcgact caggcgacgt tatccaacgc
    23401 gatggacgtg agtcagccga acaactggcc gcgtgtggaa gagttgttcc gccgcaaaat
    23461 ctggcaactg aaagagctgg gttatgcagc cgtggatgat gaaaccacgc aacagacaat
    23521 gcgtgagtta aaagaactgg gctacacctc ggagccgcac gctgccgtag cgtatcgtgc
    23581 gctgcgtgac cagttgaatc caggcgaata tggcttgttc ctcggcaccg cgcatccggc
    23641 gaaatttaaa gagagcgtgg aagcgattct cggtgaaacg ttggatctgc caaaagagct
    23701 ggcagaacgt gctgatttac ccttgctttc acataatctg cccgccgatt ttgctgcgtt
    23761 gcgtaaattg atgatgaatc atcagtaaaa tctattcatt atctcaatca ggccgggttt
    23821 gcttttatgc agcccggctt ttttatgaag aaattatgga gaaaaacgac agggaaaaag
    23881 gagaaattct caataaatgc ggtaacttag agattaggat tgcggagaat aacaaccgtc
    23941 gttctcatcg cgtaatctcc ggatatcgac ccataacggg caatgataaa aggagtaacc
    24001 tgtgaaaaag atgcaatcta tcgtactcgc actttccctg gttctggtcg ctcccatggc
    24061 agcacaggct gcggaaatta cgttagtccc gtcagtaaaa ttacagatag gcgatcgtga
    24121 taatcgtggc tattactggg atggaggtca ctggcgcgac cacggctggt ggaaacaaca
    24181 ttatgaatgg cgaggcaatc gctggcaccc acacggaccg ccgccaccgc cgcgccacca
    24241 taagaaagct cctcatgatc atcacggcgg tcatggtcca ggcaaacatc accgctaaat
    24301 gacaaatgcc gggtaacaat ccggcattca gcgcctgatg cgacgctggc gcgtcttatc
    24361 agtatctacg tgattcctgc aatttattga atttgttggc cggataaggc atttacgccg
    24421 catccggcat gaacaaagct tactttgtct gcaatctgaa tcggggctat cgtgcccagt
    24481 ttattcttta ctgccagccg taacgacggc tataaaatcc tttcaccaac tgagttaatg
    24541 tcatataccc tgccagaatc gcaaccagcc acgggaaata gcttaacggc agcgcctgca
    24601 gttgcagata actggccagc ggtgaaaacg gcaatgcgat cccgacaatc atcacgatca
    24661 cggtcatgat cattaacggc cacgatgcac agctctgaat aaacggcaca cggcgggtgc
    24721 ggatcatatg cacaatcagc gtttgcgaca gtaagcccac cacaaaccat cccgactgga
    24781 acagcgtttg cgtttccggc gtgttggcat ggaataccca ccacatcagg caaaacgtca
    24841 gaatatcgaa gatcgagctg atcggtccga agaagatcat aaagcgcccc agatccgccg
    24901 gattccaacg ctgcggcttt tgaatttgct cgtcgtcgac gttatcaaac gggatcgcca
    24961 cctgtgacac atcatacagc aggttctgaa taagcaagtg taacggcaac atcggcagga
    25021 agggcaagaa agcactcgcc accagcacgc tgaacacatt accgaagttg gagctcgccg
    25081 tcattttgat gtatttcagc atgttggcga aagtgcgacg tccctcaata accccctctt
    25141 ccagcaccat caggcttttt tccagcagga tgatatcagc cgcttcacgg gctatatcta
    25201 ccgcgccatc cacagaaatg ccgatatccg cagcgcgtaa ggcgggcgca tcattaatac
    25261 catcgcccat aaagccaacc acatgccctt cgcgcttcag taaggtcaca atgcgttctt
    25321 tatgcatcgg cgtcaggcgg gcaaacagcg tggtgcgctg tgcgagattt gccagttcgt
    25381 cgtcagatag cgtttcaata tcactaccaa tgaccacctc tcccgcgtcc agccccactt
    25441 catggcacac tttcgctgca actaactcac tgtcgccagt aaggattttt acggtaattc
    25501 cactcgcttt taatgccttc agtgccggag cagttgtctc ttttggcgga tcaaggaaag
    25561 caatatatcc ttcgaggatc aggtcggatt catccgcccg ctggtaatct ccttcacgcg
    25621 ctggcaggta tttcgtcgcc accgcaacca cgcgcagccc ctgacgatta agcgtatcag
    25681 taacccgctt aatcttacgc agcatgatgt catcgagcgg cacaatctcg ccattgtgac
    25741 gcacctgcga acacacattg aggatttcct gcaatgcacc tttgcaaacc agctgatggt
    25801 gctcggtatt ttctgccact accaccgaca tccggcgacg ctcgaaatca aacggaatct
    25861 catcaatttt ttgccaacgg ctggccagag agcgcgctga ctcttcatcc gtaccttcga
    25921 gcaccgctgt atcgagcagg tttttaagtc cggtctgata atgactgttc aaccacgcgc
    25981 tatgcagcac gcgttcgctg gttttaccgg agatatcggt atgattctcc agcacaattt
    26041 tgtcctgtgt cagggtgcca gttttatcag tgcacagaat atccattgcg ccaaagttct
    26101 gaatagcatc cagatgtttg acgatcactt tctgtttcga cagctttact gcgccgcgcg
    26161 ccagcgtcga ggtaacaatc atcggcaaca tttccggcgt taagcctacc gcgaccgaaa
    26221 gcgcaaacag tgccgcttcc caccagtcgc ctttggtgta accgttgata agcaacacca
    26281 ccggagccat caccagcata aagcgaatca gcagcatact gacgcggctg atcccctgct
    26341 gaaaggcatt cggctcgctt tcctgctcac taacacgccc cgccagttga ccaaaccagg
    26401 tattggctcc tgtagcaatc accattgctt gtgccgtgcc gctcaccacg gtggtgccca
    26461 taaaacacag ggtgtcacac tccagcggat tgctgtgctc cggctggcga gtggtagcag
    26521 ctttttctac gggcagagac tcaccggtta acgacgcctg agcgacgaac agatcccgcg
    26581 cctgcaagat acgtaaatct gccgggatca tatcccccgc cgccagtttt ataatatcgc
    26641 cgggcaccag ctggtcgatc gggatctcca gccagccatt ttcgcctttg tcgttaatta
    26701 cgcgcagcac cgtcgcggta ttgctgacca tcgctttcag ggcatctgcc gctttagtgg
    26761 aacgtgcttc ctgaataaag ttcagcaacg tagaaatagc gaccattagc gcgataacgc
    26821 ccgcggcaaa taaatcttcc gtggcgtaag aaatagcgcc gagaatggtg agtaaaatat
    26881 taaaggggtt gcgatagcag acccataaat gtacccacca cggcgacggt tgttgtgcgg
    26941 gtaatttatt ttcaccatgt tgttcgcggg cagattccac ttccgcctga tttaaccctt
    27001 ccggatgggt gtcgaacgtt ttccacaatt cttcttcggg catcaccgcc attttcaggc
    27061 aatgcgcact taaggacggc gggaccaccg tattcactgt ctgctgcgcg ccaggcaatg
    27121 gatcacgatg aaccagacgg gaaggtaaat ggcgaatgag ccgggtaaag atttctttaa
    27181 acataaggag tccctccgca ctgtctgaaa cagtacagaa atgtcttcag gcgtggcgaa
    27241 gccttacctg aaggtaataa agctgagcag ggacgttaaa acgtcacggc cttacgcatc
    27301 cggtaacgcc gtgaaaagac gggcttaccg gaaaagtttt aatctccgtc gagggagagg
    27361 cgtgggatca ggttccatat aacctccggt aagtgaaaaa agcgccgcaa ataataagga
    27421 gaatccaata atattcgtag catctatagc gatataatac ctgctggcaa ctaaaccaac
    27481 gataaaccag actttaccca ttgctgaatg cacgggtaaa gttaggctca aataattaaa
    27541 caacacgtta caggacaaca ggatgcaaaa tcggctgacc atcaaagaca tcgcgcgctt
    27601 aagcggcgtg gggaaatcta cggtttcccg ggtgctgaat aacgaaagcg gcgtgagcca
    27661 gcgcacccgc gagcgtgttg aagcagtgat gaatcagcat ggattttccc cttcccgctc
    27721 tgcgcgcgct atgcgtgggc aaagcgataa agtggtcgcc atcattgtta cccgcctgga
    27781 ttcgttgtca gaaaatctcg ccgttcaaac catgctgcca gcgttctatg aacaaggtta
    27841 cgacccaatc atgatggaaa gtcagttttc cccgcaatta gttgccgaac atttgggggt
    27901 gctgaaacgg cgtaatatcg acggcgtagt gctgttcggt tttactggca taacagaaga
    27961 aatgttagcc cactggcagt catcgctggt tctgctggcg cgtgacgcaa aaggctttgc
    28021 ttcggtctgt tatgacgacg aaggggcaat caagatcctg atgcaacggc tgtatgacca
    28081 ggggcatcgt aatatcagtt atctcggcgt gccgcatagt gacgtgacaa ccggtaagcg
    28141 acgtcacgaa gcctacctgg cgttctgcaa agcgcataaa ctgcatcccg ttgccgctct
    28201 gccagggctt gctatgaagc aaggctatga gaacgtggca aaagtgatta cgcctgaaac
    28261 tactgcgtta ctgtgcgcaa ccgacactct ggctcttggc gcaagtaaat acctgcaaga
    28321 gcaacgcatc gacaccttgc aactggcgag cgtaggtaat acgccgttaa tgaaattcct
    28381 tcatccggag atcgtcaccg tagatcctgg ctacgccgaa gctggacgcc aggcggcctg
    28441 ccaattgatc gcacaggtaa ccggacgcag cgaaccgcaa caaatcatca tccccgccac
    28501 cctgtcctga tcgtttcctg aacgataaat tgtgatcttc gctgcgcttc gggaacgttc
    28561 ccgtttttaa atttttccgc gcaatatatt ctgcagccaa ccaaaaatgt catctgccat
    28621 ggggctttat gatgagcaaa ataaaccaaa cggatatcga tcggttgatt gaactggtcg
    28681 gcgggcgcgg caatattgcg acggtgagcc actgtattac tcgcctgcgc tttgtcctca
    28741 accaaccggc caatgccaga ccgaaagaaa ttgagcaact ccccatggtg aaaggctgtt
    28801 tcaccaatgc cgggcaattt caggtggtga ttggcaccaa cgtgggtgat tactatcaag
    28861 cactaatagc gtcaaccgga caggcgcagg ttgataaaga gcaggtaaaa aaagccgccc
    28921 ggcagaatat gaaatggcat gagcagttga tctctcattt cgcggagatc ttcttcccgt
    28981 tgctgcccgc gttgattagc ggcggtttga tcctcggttt tcgcaatgtg atcggcgatt
    29041 tgcccatgag caacggtcag acgctggcgc aaatgtaccc ttccctgcaa acgatctacg
    29101 attttctgtg gttgatcggt gaagcgatct tcttctacct gccggtcggt atttgctggt
    29161 cagcggtgaa aaaaatgggc ggcacgccga tccttggtat cgtgcttggc gtgacactgg
    29221 tttctcccca gctgatgaac gcttatctgc tcgggcagca gctgccggaa gtgtgggact
    29281 ttggcatgtt cagcatcgcc aaagtgggct atcaggcgca ggtgatcccg gcactgttag
    29341 ccgggctggc actgggcgtt attgaaactc gccttaaacg catcgtgcca gattacctct
    29401 atctggtggt ggtgcccgtc tgttcgctga tcctcgcggt gttcctcgcc catgcgctga
    29461 ttggtccatt tggtcgcatg attggtgatg gcgttgcctt tgcggtacgt cacctgatga
    29521 ccggcagctt tgctccgatt ggtgcggcat tgtttggctt cctgtacgcg ccgctggtga
    29581 tcaccggcgt acaccagacc acccttgcta ttgatttgca gatgattcaa agcatgggtg
    29641 gcacgccagt gtggccgctg attgcgctgt cgaatatcgc tcagggctcc gccgtgatag
    29701 gcattatcat ttccagccgc aagcacaatg aacgcgagat ctccgtgcct gccgctatct
    29761 ccgcctggct tggggtcact gagcctgcaa tgtacggcat caacctgaaa tatcgcttcc
    29821 cgatgctgtg cgcgatgatt ggttctggtc tggcaggatt gctatgcggc ctgaacggcg
    29881 ttatggcgaa tggtatcggc gtagggggcc tgccgggaat tctctcgatt caaccgagct
    29941 actggcaggt atttgcgctg gcaatggcta tcgccatcat catcccgatt gtactcacct
    30001 cgtttatcta ccagcggaaa taccgcctgg gcacgctgga cattgtttaa ttttcttcgg
    30061 ggcgcaattg cgccccctcg catttgcagg aataacgtaa tgactaatct tccccactgg
    30121 tggcaaaacg gcgttatcta ccagatttat ccaaagagtt ttcaggacac cacgggtagc
    30181 ggcaccggcg atttacgtgg cgttatccaa cgcctggact atctgcataa actgggcgtt
    30241 gatgccatct ggctaacccc cttttatgtc tctccccagg tcgataacgg ttacgatgta
    30301 gcgaactata cggcgattga tcccacctac ggcacgctgg acgattttga cgaactggtg
    30361 acgcaggcaa aatcgcgcgg gattcgtatc attctcgata tggtgtttaa ccatacctct
    30421 acccaacatg cctggtttcg cgaggcgctg aacaaagaaa gcccttaccg ccagttttat
    30481 atctggcgcg atggcgaacc agaaacgcca ccgaacaact ggcgttcaaa atttggcggt
    30541 agtgcgtggc gctggcatgc ggaaagcgaa cagtactatt tgcatctctt tgcaccagaa
    30601 caggcggatc tcaactggga gaatccagcg gtacgcgcag agctgaaaaa agtctgtgaa
    30661 ttctgggccg atcgtggggt cgacgggttg cgcctggatg tggtgaatct gatctccaaa
    30721 gacccgcgtt tccctgaaga cctggatggc gacgggcgtc gcttttacac cgacgggcca
    30781 cgagcacacg agtttttgca cgagatgaac cgcgatgtgt ttacgccacg cgggttaatg
    30841 accgtaggtg aaatgtcctc caccagcctt gagcattgcc agcgatacgc ggcactgaca
    30901 ggcagtgaat tgtcgatgac ctttaatttt catcacctga aggtcgatta tcccggtggt
    30961 gaaaaatgga cgctggctaa acctgacttt gtggcgttga aaacattgtt ccgccactgg
    31021 caacaaggaa tgcacaacgt ggcatggaat gccttgttct ggtgtaacca cgatcagccg
    31081 cgcattattt ctcgctttgg tgatgaaggt gaataccgcg tgcctgcggc aaaaatgctg
    31141 gcgatggtgc tgcatggcat gcagggaacg ccgtatatct accagggcga agagattggc
    31201 atgaccaacc cgcatttcac gcgcattact gactatcgcg acgtggagag cctcaatatg
    31261 tttgccgagc tgcgcaacga tggtcgtgat gccgacgagt tattggcaat cctcgccagt
    31321 aaatcccgtg acaacagccg cacgcccatg caatggagca acggcgataa tgccgggttt
    31381 acggctggcg aaccgtggat tggcctgggc gataactatc aacaaatcaa cgtagaagcc
    31441 gcgctggccg atgagtcctc ggtgttttac acctaccaaa agttaatcgc actgcgtaag
    31501 caagaagcca tcctgacatg gggcaattac caggatctgc tgccaaacag ccctgtattg
    31561 tggtgctatc gccgtgaatg gaaggggcaa accttgctgg tcattgccaa ccttagccgt
    31621 gagatccaac cctggcagcc agggcaaatg cgcggcaact ggcagcttgt gatgcataac
    31681 tacgaagaag cctcaccaca accctgtgcc atgaatttac ggccttttga ggctgtctgg
    31741 tggttacaga agtaaatctt ccctaagccc cggtaatgcc ggtcattccg gggttttgtc
    31801 tgtcagcatg ttgtttttgt tgatttttca accagcaaat tcattaaaaa atttacataa
    31861 cgctgtagcg cccgtcatcc gtacgctctg ctttttactt tgagctacat caaaaaaagt
    31921 taaaacatcc ttgatgcaaa gcactatata tagactttaa aatgcgtccc aacccaatat
    31981 gttgtattaa tcgactataa ttgctactac agctccccac gaaaaaggtg cggcgttgtg
    32041 gataagcgga tggcgattgc ggaaagcacc ggaaaacgaa acgaaaaaac cggaaaacgc
    32101 ctttcccaat ttctgtggat aacctgttct taaaaatatg gagcgatcat gacaccgcat
    32161 gtgatgaaac gagacggctg caaagtgccg tttaaatcag agcgcatcaa agaagcgatt
    32221 ctgcgtgcag ctaaagcagc ggaagtcgat gatgccgatt attgcgccac tgttgccgcg
    32281 gttgtcagcg agcagatgca gggccgcaac caggtggata tcaacgagat ccagaccgca
    32341 gttgaaaacc agctgatgtc gggtccatac aaacaactgg ctcgcgctta catcgagtac
    32401 cgtcacgatc gcgacattga acgtgaaaaa cgcggtcgcc tgaaccagga gatccgtggt
    32461 ctggtcgagc agaccaacgc ctcgttactc aacgaaaacg ccaacaaaga cagcaaggtg
    32521 attccaaccc agcgcgacct gctggccggg atcgtggcta aacactatgc acgtcagcac
    32581 ctgctgccgc gtgacgtggt gcaggcacat gagcgtggcg atattcacta tcacgatctc
    32641 gattactcgc cgttcttccc gatgttcaac tgcatgttga tcgacctgaa aggcatgctg
    32701 acccagggct ttaaaatggg gaacgccgag attgaaccgc cgaagtcgat ctctacggca
    32761 accgcggtaa ctgcgcagat tattgctcag gtcgccagcc atatttatgg cggcaccacc
    32821 attaaccgta tcgatgaagt gctggcgccg tttgttaccg ccagctacaa caaacatcgc
    32881 aagaccgcag aagagtggaa catcccggac gccgaaggct acgctaactc tcgtaccatc
    32941 aaagagtgct acgatgcctt ccagtcgctg gagtacgaag taaacaccct gcacaccgcc
    33001 aacggtcaga cgccgtttgt aacctttggt tttggtctgg gcaccagctg ggaatcgcgc
    33061 ctgattcagg aatcgatcct gcgtaaccgt atcgcaggcc tcggtaaaaa ccgtaaaact
    33121 gcggtgttcc cgaaactggt gtttgcgatt cgcgatggtc tgaaccataa aaaaggcgat
    33181 ccgaactacg acatcaaaca gctggcgctg gagtgcgcaa gcaagcgcat gtatccggat
    33241 atcctgaact acgatcaggt agtgaaagtc accggttcgt ttaaaacccc gatgggctgc
    33301 cgcagcttcc tcggcgtgtg ggaaaatgaa aacggcgagc agatccacga tggtcgtaac
    33361 aacctcggcg tgatcagcct gaacctgccg cgtattgctc tggaagcaaa aggcgatgaa
    33421 gccaccttct ggaagctgct ggatgaacgt ctggtgctgg cacgtaaggc actgatgacc
    33481 cgtatcgctc gtctcgaagg cgtgaaagcg cgcgtcgccc cgatcctcta tatggaaggt
    33541 gcttgtggcg tacgtctgaa tgctgacgat gatgtttctg aaatcttcaa aaacggtcgt
    33601 gcgtctattt cgctgggtta catcggcatc cacgaaacca ttaacgcgct gttcggcggc
    33661 gagcatgttt acgacaacga gcagcttcgc gcgaaaggta tcgccattgt tgaacgtctg
    33721 cgtcaggcag tggatcagtg gaaagaagaa acgggttatg gtttcagtct ctacagcacg
    33781 ccgagtgaaa acctgtgcga ccgcttctgc cgtctcgata ctgctgagtt tggcgtggtg
    33841 ccgggcgtga ccgataaagg ttactacacc aacagtttcc acctcgatgt ggagaagaag
    33901 gtgaacccgt acgacaagat cgactttgaa gcgccttacc cgccgctggc gaacggtggt
    33961 ttcatttgct acggcgagta tccaaacatt cagcacaacc tgaaagcgct ggaagatgtc
    34021 tgggattaca gctatcagca tgtaccgtat tacggcacca atacaccgat tgatgagtgc
    34081 tacgagtgtg gctttaccgg tgagttcgag tgcaccagca aaggcttcac ttgcccgaaa
    34141 tgtggtaacc atgacgcctc ccgagtgtcg gtaactcgcc gcgtgtgcgg atatttaggt
    34201 agcccggatg cacgtccgtt taacgctggt aagcaggaag aagttaagcg ccgcgttaaa
    34261 catttgggga atgggcagat aggttaatct tcactctgcg ctaagcaaat gccggatgcg
    34321 gcgtaaacgc cttatccggc ctacatttgg caacggctgt aggcctgata atacgcgaca
    34381 gcgtcgcatc aggcattgat taccggatgc agcctacacg cggactgata agccaggcga
    34441 aataatgaat tatcatcagt actatcctgt cgacatcgtc aacggccccg gcactcgctg
    34501 caccctgttt gtctccgggt gtgttcatga atgccccggt tgctataaca aaagcacatg
    34561 gcgggtaaat tctggtcagc catttaccaa agcaatggaa gaccagatca ttaacgatct
    34621 gaatgacact cgtatcaaac gccaggggat ttccctctcc ggcggcgatc cgctgcatcc
    34681 gcaaaacgtg ccggatattc tgaaactggt aaaacgcatc cgcgccgagt gtccgggtaa
    34741 agacatctgg gtgtggacag gctataaact cgacgatctc aacgctgcgc aaatgcaggt
    34801 tgttgatttg attaacgtac tggtcgacgg caaatttgtg caggatttaa aagacccttc
    34861 cctgatctgg cgcggcagca gcaatcaggt ggtgcatcat ttgcgatgat aagttccctg
    34921 ttacctgagt gcaggcaaca gggaaatgag gaattaacga tacttctggt gataggcgtt
    34981 gcgggtcgtt ttcagttgct ctgcggcagc ctgcgcttct tttactttac cttcatttgc
    35041 cagcttcagc gcgtcgtcaa tctgaccaac cagaatgtcg aaaccgtggc ggaagtcttt
    35101 catttccggg ctgtctggcg attttccttc cagcttcggc ggcgttgctt tttgcgcatc
    35161 cagcgccgcg gcacgcattt tggttaacgc gtctttgact tgcgccgcgt tatccgcttt
    35221 ttcgaccact tttaaattgt cgttgagggt ttccatattg tcttcaagat cagcggcaaa
    35281 cgacgccgaa ctgaatacca atgaggagac tgcaagaata gctaacaggc ttttacgcat
    35341 tgctcacttc cttttttatt tacacttcat ccttcacact gcctcatcga cggctacgct
    35401 cattcgcccg agtaacgtac tacgagtacg ctccccggcc ctcattcact ttccgtctcg
    35461 atgcagcgcg aatgattttg tgtaattttt tagtttatag cgcggcaggt cgcgccagtt
    35521 tttttactgt ccggcgattt tcatctccgg caacagcaca gaaccacact gtatattact
    35581 gcgtgtttca atatcgttac cgacggtgac aatattgcgc cacatatctt ttaaattacc
    35641 tgcgatggtg atttcgctaa ccggatactg aatttcgcca ttctctaccc agaagcccgc
    35701 agcaccacgg gaataatcac cagtaatggc actcacgccc tggcccatca gctccgtcac
    35761 caccagcccg gtgcccatct ctttgagcat ttgttcgaag cttagccctt gtccggcaat
    35821 ccgccagttg tgaataccgc ccgcatgtcc ggtgcttttc agccccagtt tccgcgccga
    35881 gtagctggtc agcagccact gagtcaggat gccatcttta ataatatcgc gacgctcggt
    35941 gcgcacacct tcgctgtcga atggcgtcga cgccagccct ttcagcagat gcggatgctc
    36001 ttcaatggtc agccagtccg gcagaatttg tttacccagc gaatccagca gaaaggtaga
    36061 tttacggtaa accgagccac ccgctatcgc ccccaccaga tggccaaaaa gcccggttgc
    36121 cacttcattg gcaaaaatga ctggcgcttt catggtggag agtttacgcg gtgacaggcg
    36181 cgataaagta cggcgagcac agtcggcccc gacccactct ggcgtttgca gatcgctcat
    36241 cgcgcggcca atggtgtagg cgtaatcacg ctccatatcg ccattttctt cggcaattac
    36301 acagctggag agcgaatgac gcgttgagca gtaaccctgc aacatgccgt ggctgttgcc
    36361 aaaaactttg acaccgtagt ggctgttaaa gctgccacct tcggtattgg taatgcgttt
    36421 gtccgcctgc aatgccgcct gttctgcgcg ggccgccagt tcaatggctt catccgggga
    36481 aacttccgca gggtggaaca agtcgagatc tggtgcgtca aaggccagca gctctttgtc
    36541 tgccacgccg gcacagggat ctggcgaggt ataacgggca atatccagcg ccgcctgtac
    36601 agtacgggca atggcctgcg ggcttaaatc ggtggatgat gcgctacctt tgcggttctg
    36661 gtgataaaca gtgatcccca gcgcgccatc gctattgaat tcaacattct ccacttcacc
    36721 ataacgcgtg cttacgctaa tgccggtggt cttgctgacg gcaacttccg caccgtccga
    36781 tttgcctgag gccaactcca gcgcagtgga aactgcttct tccagaatct tgcgctgcgc
    36841 ttcaacttga gagattactt tcattgcaag tgccataatg tagagagttt ctctgaagtc
    36901 taacagagaa ccgtttttca gtgcgcatct taactggtaa cattagcctc tttttttaag
    36961 gagcctgaga tgactaagca gcccgaagac tggctcgacg acgttcccgg tgatgacatc
    37021 gaagacgaag acgatgaaat tatctgggtc agtaaaagtg aaattaaacg tgatgccgag
    37081 gagctaaaac gccttggcgc ggaaattgtt gatctgggga aaaacgcgct ggataagatc
    37141 ccgttagatg cggatctgcg cgcggctatt gagctcgccc agcgtattaa gatggaaggt
    37201 cgccgccgcc agttgcagct catcggtaag atgctgcgcc agcgcgatgt agaaccgatc
    37261 cgtcaggcgc tggataagct gaaaaaccgt cacaaccagc aggtggtgct gttccataaa
    37321 ctggaaaacc tgcgcgatcg tctgatcgat caaggtgatg acgccatcgc tgaggtatta
    37381 aatctgtggc ccgatgccga tcgtcagcag ctgcgtactc tgatccgcaa cgcgaagaaa
    37441 gagaaagaag ggaataagcc gccgaaatcc gcacgccaga ttttccagta tctgcgcgaa
    37501 ctggcggaga atgaagggta attttcgttg ttgatgtgag tatggtttgc cggatgcggt
    37561 gcacctgtag gcctgataag acgcatcaag cgtcgcatca ggcactatgc tcaaatgccg
    37621 gatgcggcgt gaacgcctta tccggcctac gcacactata tcaggctgag gccgaattac
    37681 tgcgcagctt ccgccttctt cgccagacca tccagcagtt tctgatggat cccaccaaaa
    37741 ccgccgttgc tcatcaccag aatatggtcg ccaagctgag cggttttcac caccatatct
    37801 gccagcgtat ccacatcgcc actccagtgt gcaggctgaa cgcaggcttc tgccacttct
    37861 gccacctgcc acggaatatg cgccggttgc agcaggaaga cttcatcggc acgacctaat
    37921 gaaggtgcca gatcgtcttt gcagatcccc attttcatgg tattcgagcg cggttccagc
    37981 acagcaataa tgcgcgccgt accaccaact ttgccacgca gcgccgccag cgttgccaga
    38041 atcgccgtcg ggtgatgggc aaaatcgtca tataccgtga cgccattcgc ttcaccacgc
    38101 aactccagac ggcgacgagc attaataaac gaacccagcg cgttagcggc atctgccggc
    38161 gctacaccaa catggcgagc cgctgcaatc gccatcaggc cattgtgcat attatgttcg
    38221 cctaccagcg accatttcac ttcgcccact ttttcgccat ccagcaaaac ttcccattcg
    38281 gaagcatcgg tggtcagctt tttcgcctgc cagtgaccct gctcacccac cagctcctgc
    38341 tcgctccagc agcccatcgc catggtctgt ttcaggttga tgtcattttc cggccagata
    38401 atacggccct gccccggaac gatacgcacc agatggtgga actgtttctg gatcgctttc
    38461 aggtcgtcaa agatatcggc gtgatcgaac tcaaggttgt tgaggatcag cgtacgcggg
    38521 cagtaatgga caaatttaga gcgtttgtcg aagaaggcgc agtcgtactc atccgcttcg
    38581 ataacaaaga agtcgctttc gcccagacgc gccgaaacct caaagttccc cggcacaccg
    38641 ccgattacaa agcccggttt gtaaccacac tgttccagaa tccaggtcgc cattcccgcg
    38701 gtggtggttt tgccgtgtgt acctgcaacg gccagcaccc agcggtcgcg cagcacaaaa
    38761 tcgtgcagcc actgtggacc tgacatataa gggatgtttt tttccagtac cgcttccaca
    38821 cacggatttc cacgggtcat ggcgttgcca ataatcacca gatccggctg cggatcgagc
    38881 tggctggcat cgtaaccctg aatcagctca atgccttgct tctcaagtaa ggtgctcatc
    38941 ggcggataca cattggcgtc cgaacccgtt acttcatggc ctaactggcg cgccagcatc
    39001 gccagaccgc ccataaacgt gccacaaatt cctaaaatat gaatgcgcat acgtcactat
    39061 ccttttttaa tctgccgctc attttacgca tatgtcgggc aagtgagaaa cgcatttcag
    39121 gataatccgc aatttgctgg cgcgattcac ctgagcgcaa cattgagatt atttgttaag
    39181 attgttgcgg tcgctttact ccataaacat tgcagggaaa gttttatgaa aacgttaggt
    39241 gaatttattg tcgaaaagca gcacgagttt tctcatgcta ccggtgagct cactgctttg
    39301 ctgtcggcaa taaaactggg cgccaagatt atccatcgcg atatcaacaa agcaggactg
    39361 gttgatatcc tgggtgccag cggtgctgag aacgtgcagg gcgaggttca gcagaaactc
    39421 gacttgttcg ctaatgaaaa actgaaagcc gcactgaaag cacgcgatat cgttgcgggc
    39481 attgcctctg aagaagaaga tgagattgtc gtctttgaag gctgtgaaca cgcaaaatac
    39541 gtggtgctga tggaccccct ggatggctcg tccaacatcg atgttaacgt ctctgtcggt
    39601 accattttct ccatctaccg ccgcgttacg cctgttggca cgccggtaac ggaagaagat
    39661 ttcctccagc ctggtaacaa acaggttgcg gcaggttacg tggtatacgg ctcctctacc
    39721 atgctggttt acaccaccgg atgcggtgtt cacgccttta cttacgatcc ttcgctgggc
    39781 gttttctgcc tgtgccagga acggatgcgc ttcccggaga aaggcaaaac ctactccatc
    39841 aacgaaggaa actacattaa gtttccgaac ggggtgaaga agtacattaa attctgccag
    39901 gaagaagata aatccaccaa ccgcccgtat acctcacgtt atatcggttc actggtcgcg
    39961 gatttccacc gtaacctgct gaaaggcggt atttatctct acccaagcac cgccagccac
    40021 ccggacggca aactgcgttt gctgtatgag tgcaacccga tggcattcct ggcggaacaa
    40081 gcgggcggta aagcgagcga tggcaaagag cgtattctgg atatcatccc ggaaactctg
    40141 caccagcgcc gttcgttctt tgtcggcaac gaccatatgg ttgaagatgt cgaacgcttt
    40201 atccgtgaat tcccggacgc gtaatctcct gcgcctctcc acacggagag gcgtttttat
    40261 cgctgcacaa tattgactct tgtcactggc gaactctgac gattctccca cagcaccgtt
    40321 aatccacgct gtaaggcaat aaaaatgaac agcaaaatgc cgatggcgat tttcgtccac
    40381 caggaactca gcgtaccgtc aaagttgata taagtctgaa ttagcccctg aatcgccacg
    40441 ccaaaaagcg tccctaatac cgttccaacg ccaccgctca aaagcgtacc gccaattacc
    40501 actgaggcga tagcatccag ttccacacct acgcccgcca gcgcatatcc ggcctgggta
    40561 taaatcgaga agacaatccc cgccagcgtt gccagtccgg tggagagcat ataaatgcga
    40621 atagtggtgc tgcgggtaga aatccccatc aggtttgccg acgttgcgtt gccgccaatg
    40681 gcgtatacct gattaccaaa acgggtacga tgcgcgagga atatgccgat aaccaccacc
    40741 gccagcatca gcagtcccat cgcacttaag cgaccgccgc cagggatttt ccacgcaagg
    40801 cttgagagcg tgtcataaat gggatggttt atcgggatcg actcttctga aacgagatag
    40861 ctgacgccgc gcaaaaagaa cattcctgcc agggtaatga taaatgccgg gatcttcagg
    40921 gcgtcgatca aaagccccat aaatgcgcca aaggcacagc ccatcaccaa caccagcgga
    40981 aacgccagca gcggcgagag gccgaaatcg ccaatcactt ttgccagaaa cacgccagta
    41041 aaggcgatca ccgaaccgac ggagagatcg ataccgccag agaggatcac aaaggtcatg
    41101 ccaacggcga ttatcccaag aaaggcgtta tcggtcagga tattgcagat cactcttgtg
    41161 gaagcaaaac cgggaaactg agtcaggcag tagagatagc ccagcacaaa gacgccgatg
    41221 gtgatcatca gcggcaagtt acgttttatc atggctacgt actcctttaa tcagactgat
    41281 aaagcgttgc gactgaacaa tcagcacaca aagcaccacc accgctttca ccacctggtt
    41341 catctccggc ggaaagcccg aaagcaaaat tccggtgttc atcccctgaa taatcagcgc
    41401 ccccactacc gagagcagca ggttaaagcg cccgcccatc agcgatccgc cgccaatcac
    41461 caccgcgaga atggcgtcca gctccagcca taatccggcg ttgttggcat cggcaccgcg
    41521 aatatccgcc gccacgataa tgcccgcaat cgccgcacac agcccgctca acacataagt
    41581 gagcatgacg atgattcgcg tgtttacccc ggcatttttt gccgcccgaa tgttgatacc
    41641 aacggcttcg ataaacatcc ccagcgccgt tttgcgggtc aacagccaga acaggagaag
    41701 cgtcagcacc gcgataatga ccggcgttgg caggaacaac agcgatccgc tgccaaacca
    41761 tgagagatcc ggcgagttaa acgtgacgat ctgcccggag gtgatcagtt gcgccacgcc
    41821 gcgcccggcg accatcagga tcagggtggc gacaaacggc tgaattttga ggatcactac
    41881 cagtatgccg ttccacaatc ccgccaggat gccagtgccc agggcgctta acaaaacaat
    41941 cggcaggctg aatcccgcga ccgtcatcgc agccgttgtg gctccggcga tagccatcac
    42001 cgcccctacg gagagatcaa tcccaccggt ggcgatcacc agcgtcatac caatcgccag
    42061 taacgcaacg ggagctgcgc ggttaagaat gtcgataggg ctaccgaaca aacgcccatc
    42121 ctgaagcacc acctgccaga aatgcggagc taccaggcta tcgaccagca gcaccagcaa
    42181 tagcgccgcc agctgcggca ttcccgttgg ccagcgaaag cgccttttcg gcggcgtagt
    42241 gtccgggaga gattgaggca tcacactgtt ctccttacgc cgcaatggcg ttcatgatcg
    42301 ccggaacgga aagctctgcc agcgggatct ccgccacctg tttgcgatcg cgcatgatga
    42361 tcacccgatc ggcatagccc accagctctt ccagttcgga ggagatcacc agcagcgcca
    42421 gaccatcggc gcatagcgtt tcaatcaggc ggatgatctc ggcgtgggca ccaacatcaa
    42481 tgccgcgggt tggctcatcg aggatcagaa attgcggtcg ggtcagtagc caacgtgaaa
    42541 gcaacacttt ttgctgattg ccgccagaga gaaattcaat cggttgttca gttgaaggtg
    42601 tgcgaatgcc aagctggcgg ataaagcgtt cggcaatctc ttgctgctct ttgcgggaaa
    42661 tgggtcgtag ccagccgcgc tgggcctgga gagcgaggat gatattttcc cgtaccgagg
    42721 cggcggcgat gatgccatcg gttttcctgt cttccgggca gaagccgatg cccagtaccg
    42781 aagcctgatg tggcgatcgc aggttttgct gtttgccttt gatcaacgcc gtgccgctgt
    42841 cagcaggttt gataccgaag atcacttcgg cggtttcggt acgtccggat cccagcaagc
    42901 ctgccaggcc gacgatctcg ccggggcgta cttcgagatc aaacggtgcg atcgttcctt
    42961 ttttgccgta atttttgaac gcggcaacgg gtttgtcgct caacaatgtt cgcccggcac
    43021 gctgtagcgc gtgggtatcc agctcgcgcc ccagcatcat ttttaccagt tcgatctgcg
    43081 gtagctcgcg cgtttcccga cagcctacga aactgccgtt gcgtaagacg gtgatccgat
    43141 cgctgacctg atagacctga tcgagaaagt gagtgacaaa gatcaggctg acgccgcgat
    43201 cgcgcaactg acgcatcagg ccaaacagta actctacttc ctgggtgtcg aggctggcgg
    43261 tgggttcatc gaggatcagc actttggcgg agagatcgat agcccggcaa atggcgacga
    43321 tttgctgcat cgccaccgaa aaacggttaa gcggttcacg cacgtcgagg gagaaaccgt
    43381 aagatgtcat cagttcggtg gcacgctttt ccatctcttt gcggcgtaga aagccgaaac
    43441 gtttgggttc gcgacctatg aatagattat cagcgaccga catattgggt agcaggttga
    43501 cttcctgata aacggttccg atgccgagtt gttgtgcatg ggcggtattt ttcggtgaga
    43561 tagcctggcc ttccagccag atggtgccgc gatcggcgtg gtatacaccg gttaatgctt
    43621 tgattagcgt cgattttccc gccccgtttt caccgagcag cgccatgatt tcgccacggc
    43681 gcaggctgaa atcaacgttg tctaacgctt tgacgccggg gaaaaattta cttaatcctt
    43741 cggtgcggag gatctcctgg tgttggtcgg tcgtcatggt ttcccccggt gtaaagcgtg
    43801 ctcccagtag gcatgataag acgcggtaag cgtcgcatca ggcttcagtg caccatcgcc
    43861 ggatgcggcg cgagcgcctt atccggccta cgaatcggca tcaggcatag caaccctcaa
    43921 taccccatat ttttcttctt ctctaactct tcttttgcgg tatcaggcag ataaagggtg
    43981 gatttggtca gcgtcagctt ttcaggcatg gtgccgtctt ttttgtattt ctccagcgcg
    44041 tcgaaggcgg gacctgccat attcggcgtc agttcaacac tggcgttcgc ttcgccatca
    44101 atcatcgctt tgtagatatc gggtacaccg tcaatggaac ccgtgaggat atctttgccc
    44161 ggtttcaggc ccgcttcttt aattgcctga attgcgccaa tcaccatgtc atcgttatgg
    44221 gcgtaaacca tgcagatgtt tttgccgttg ttttccgctt tgataaagct ctccataact
    44281 tctttgcctt tactgcgggt gaagtcacct gactgcgagc ggatgatttt gatatttggc
    44341 gcattcttaa tggcttcggc aaagcctttc ttacggtcaa tggcgacgct ggccccaacg
    44401 gtgccctgca gctccaccac gttgcatggt ttgccattca cttcttttac cagccagtca
    44461 ccgatcaact taccttcgag gatgttgtcg gcagtaacgg tggtcatata gagagatttg
    44521 tctttcacat cgatggaacg gtcgagcaag aagaccggga tttcggcatc tttcgcctct
    44581 tttaataccg gttcccaacc tgtcgcgacc accggagcga taaagatcgc atccacccct
    44641 tgtgcaacga aggaacgtac cgctttaatc tggttttcct gcttttgctg accatcagca
    44701 attttcaacg taattccgcg tttttcggcc tcacttttcg ctacattggt ttctgcggcg
    44761 cgccagccgg attccgatcc gacctgcgaa aatccaacgg ttaatggagc ggccaacgcc
    44821 atagacgaca tggctgccga gactgcagag actataagta agcgtttcca catagggttg
    44881 tcctcgtcgg ggtaattatt attggttaaa agatgttcgc gaaaaaacta tagacaattc
    44941 gttatgtaac ggattgcgtt acatcacact tcaaaaaagt aaataaaacg ttaatcacaa
    45001 gtttgtaatc gctttcatct cactatgaaa aatgcggcta cggttatgga ttttcctgct
    45061 ctgcataccg tcttaaaact ggcgaaaaag gaaaatgaag acgaaaacaa gcgaagacat
    45121 tcggcgcgag ttggctataa tactcggcac ttgtttgcca catattttta aaggaaacag
    45181 acatgagctt actcaacgtc cctgcgggta aagatctgcc ggaagacatc tacgtggtta
    45241 ttgagatccc ggctaacgca gatccgatca aatacgaaat cgacaaagag agcggcgcac
    45301 tgttcgttga ccgcttcatg tccaccgcga tgttctatcc gtgcaactac ggttacatca
    45361 accacaccct gtctctggac ggtgacccgg ttgacgtact ggtcccgact ccgtacccgc
    45421 tgcagccggg ttctgtgatc cgttgccgtc cggttggcgt tctgaaaatg accgacgaag
    45481 ccggtgaaga tgcgaaactg attgctgttc cgcacaccaa gctgagcaaa gaatacgatc
    45541 acattaaaga cgttaacgat ctgccagagc tgctgaaagc gcagatcgct cacttcttcg
    45601 agcactacaa agacctcgaa aaaggcaagt gggtgaaagt tgaaggttgg gaaaacgcag
    45661 aagccgctaa agctgaaatc gttgcctcct tcgagcgcgc aaagaataaa taagttctta
    45721 tagcgcaata accctgaaca ccgggcttcg gttagtaagg gtttttttat gcccgcgata
    45781 aataaactct ctattccact ttcattattc tcagcgcttg caaggcttga acggtaagaa
    45841 caagcaaacc cgaccaccat tttgctgttc atagccactt gctggaagtt agccgacctc
    45901 actcatactc accgttcaaa gcgtgccccc gttttacccc tgcacatttg tgaacgccag
    45961 cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag caaaagatgg
    46021 aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa caacgccacc cgaaggtggc
    46081 ataagggggt agcatgtacc gcactacccc ttgatatatc aaatagttac aagttcaaag
    46141 tggttcgaaa tcgtgccatt tagtccccgt ttatgggcaa aatctggaca actccgcaac
    46201 cggattgaac cttaccgcgt cctggagata atcaggtgca aagtgcgcat aggtcattgt
    46261 ttgctgaatt gtgctgtgcc ctaaaatacg ctgaagggta ataatgttcc ctccatttat
    46321 catgaaatga gttgcgaatg tgtgccgtaa aacatgcaca gactgaccga ctggtaaatc
    46381 aggccgcaca gtccttattg cacttctgac tatcgcataa ctggggttca ttaatcttcc
    46441 agtcgctttg actttaatca gagattcaag atctttagct ataggaataa cccgccgcga
    46501 tccattcttt gtctccatga aaataacctt cccaccaatt atgtgttcac ctttaatatt
    46561 ggcaacttca ccccagcgcc cgcctgtagc caggcttaac aatacagcct taagattatc
    46621 gccagataaa agtgcggtta gcctgggaat atcatcatgt gcaagataag ccatttccgg
    46681 ctgattaatt gttagcttgc gtatctcgtg aaacgggtta tcacccacaa actcttcaac
    46741 atcaatcagc ttttcaaaga aaccactcat cattgcacaa taacgattta ccgttgactg
    46801 cttaattccg cgattcatta attcaaggcg atatctgata atattttttc gggttaagtg
    46861 gtcacttctg ttaataccaa gctcttgcat atcacgaaga accgcttcaa ttctgacccg
    46921 attcatcgcg ccataacgat gattcttacc gtggtacacc caccacagtt ccagaagatc
    46981 gcttaacgct ttacgttcaa taggtttttc gacccagtcc ttatcgtgat aatgcgtcag
    47041 aacatggcgt tcatacatcg ccgcttcacc acgggtagga aatcgccgcc gaaatcttct
    47101 tccttcagat ccagcaggcc gcacgtccac acagtatttg ccatcagcga gcttcttaat
    47161 cgacataagg aagcccccca atgaaaacat catcttgatc acaattttga gaaataaagg
    47221 ccagatggac cgttaaccag tcttcttgcc ggagtgggat gattccgctt cgctttgccc
    47281 aatgtgtgcg agagccggta ccacctgccc ggcctttggt tcgacctcat cgaacaaaaa
    47341 ccagtcacgg tatttacgga agacgggaga cttgagcagc ttttttgtgg attcaagatt
    47401 tggctcattt ctcccctgtt catattgacg cagggccgct accgtgagcc ctgtaatttc
    47461 agccattttt tcttgtgtaa gcatttcaga cttacgcatg attctaattt tttcacctac
    47521 gttagttgac atagtaagtt aactctctta gtttatacat caacaaacac acccagagac
    47581 aatctaggcg gctctaactg gtgctaaata gaaatagcac caataacgga ggataccaga
    47641 tgcaagaagt cactcaaaac gcgcctgaat cgcctgccga aacacagtgc gatcagttga
    47701 cagatgcgcc agaaaccgca caccaaaaga ccgggcgcaa agcccctgaa cgccaggaaa
    47761 tcaggctttc acaaaaccct tctcatctcc tgtcaaaaga agggtttgcg atgtatatcg
    47821 gcaagaccgt tgacgccatc gtgagcatgg caaaagcagg gaaattaccc gctgtgtaca
    47881 tggcggatcc cctcaagcct ggcggaaacg ccgaacttta catcaaccgg aaagcatggg
    47941 atgaagcctg cgatcgactc actgaaaacg caccgcaaga atggcacgaa tgggaaaacc
    48001 gacttttttt atttaagcca gcagctggtt cacggaataa acgcatcagt aaaaaagctg
    48061 cgtgatatag aactggcaat cattgggagg acaggatgat taagaaatac agcgaacacg
    48121 ggagcaaggc tggcgaagca atcgccattg acgaggattg cctgataaca ggagacccct
    48181 ggctggcaac agaaagggat ttagaaggca aaacattttt tgtcagagca ataaagcaca
    48241 aagatagggt tgtgggtgga attatttatt caaccagtac gattattcct gataattatc
    48301 tggatatttc ttttagacct gtaagcattc aggggtgcga ggcatacctt aattacgttg
    48361 cggagctaaa gaaaattaac ctggactaca tgtggccaat cgtacaacgc gaaataatgc
    48421 gacgaaaatc agaaggcttc gatgtgaaaa ttgagttttt atcgacgtta atgggatttt
    48481 tacaaaaacc attgattctt ttcccctccc ttgaccggaa cctgccagtc aaagaagggg
    48541 tggaaaaata tattgttgaa caagtcagag atctggtttg tctttagagg cgcgaataaa
    48601 gcacgcaaga tgattaatac tatgaaatcg tgtccttata ttcacggcat agggagcaaa
    48661 ggcttgttgc ccctctatct gacgcaaaat ttctgacgca gttttcatca atgcatccct
    48721 tttatcatcg ggaagttcgc taatgaggag cgaaataagc agatcggaca ccactcccac
    48781 tgagtctctt gggtcagtca acttatcaac aggagccaca gcaagattag ttgtgtaaat
    48841 gctaaccagg tattcagtga tagcgtcctt aacgctttta tcaatcatgt tagttacctt
    48901 tttctggttg tgtggaaaca ccagaatacc acgcgccggg cgtggttaaa catcccggca
    48961 ctgattttca tttaacttaa acggggtttt ccgatatgac acagagacgc agcttccgca
    49021 ctggcacaga acgccacgct aaccgtttct caaccagcgc atcacgcagc aacatgcact
    49081 acagcctgca ggaaacgcac gccacacctg acgggcatct ggttaaacaa accggtaaac
    49141 acacctggct gattaatgat gccggaattg tcatacacag aggcgcacgc aacccgatta
    49201 ccggaaatac ctgttactcc ctgactcgcg gagatgagca gttcgggcag gatttcaccc
    49261 tgcacgaagc attaagaacg gcagaccggt taatcagcgg tcgcagtttt cagcacccgg
    49321 aacatcgccg ctgataaata aaaggattca gccatgatcg aagcctacaa aatacttatt
    49381 aataatgcgt tgcagcgtta tcacttcgaa ctcgcaaacc ggggtgtaac ccgggacgaa
    49441 ggacttcgta actcactgcg gggcgcaatt atgggactgt ataactgcgc actgggcagc
    49501 gatgaccgcg aagcactgga agaattaaaa gttattgtgg ccgaactgtt tgaaggtgaa
    49561 atcgttgagc cttatttcac agaggtagca gcatgagcgt atttctttca tgggtgattc
    49621 tgattatttc cgtgcttatg gcgattggca ttatgcgaat tatcgaagcg ctgaaaaata
    49681 tcgaatcttt atttactggc gaagaacgcc gtaaataaat aaccaaccgg acagaaaaca
    49741 ttaaaaccat ccgcatctgc ggagggcttc actcaatcca gaaacggaga aacagctatg
    49801 agcaccaata cacctacaga aataaacaaa ctatttaccg acccagcgca tattgaattg
    49861 acgaaaaaaa cgctgactga aataaccaat gcactggatg aaagaatatc agaaatagat
    49921 aaagacaagc attttgccac ctacatggcc ctgcaaatgc aaagcatggc aatggtgaca
    49981 gcaaaacaga ccatcagtga actctatatg aaaaacctgc gcctcgagcg cgaactggca
    50041 gaactccggg cacagtccgg ccagttttca gcgtgagggg caggacgatg aacagcatat
    50101 ttaccgccac aagacgaacc ctgctgacgc atttcaccga tgccgcaggc cgcgagttca
    50161 tggtggaaag ttatttgatc acaacgacca cgccgtgccc gtcagacgct gattatctct
    50221 acatccatct ggctgacggc acacaaatta ccgcgattgc cagtaccgtt cgcgaagtaa
    50281 tgaccatcag aggggcatgg aaatcagaaa cacaagccca cggagagctg cggccatgat
    50341 cagcaagtac accaccaacg cacgtaaacc cggtaaagac gcacgccacc acatcgccgt
    50401 actccgcaat ctgttactgg aggaaatcga cgacttcacg gcaacgctga gcggtccggg
    50461 cgcaccagta acaccaggtg agatccaccg ggaccttaaa cgccgtattg aaaacgccat
    50521 tgattacacc ctgaacccga ctgaataacc ggaactgcaa aggagatctc acgatgacca
    50581 ttctggacta catcgcagcc aatccggggt gcagcggaag tgaaatcgcc acggcactga
    50641 atacccagac cacagccatt aatgctgagt tacgccgact ctggcgcggc ggcttagtca
    50701 tcagaacaaa ccgcagcaca ggtggtcgcg ctcgcaaaac aggaggccag gcttcttacc
    50761 acgtaaaccc gatgccgttc gggtgtagca atccacttac tcacatgttt aaccagctac
    50821 tgaaggaagc cagagcatga gcaccatcaa ccaccagaag ctacgcgaac tggcatttgc
    50881 cctgcaacga atggcaacgc ctcaaaaatt actggcattt cgcgcaatgc tctcgccgtc
    50941 tgctgtgctg gcactgctgg atgagctgga gcacgccaga accacgactc ctgccattcg
    51001 cctgacgctc catcatgaaa tcgctgattt ctgcgcgacg ttggaggcgc caggtgaacc
    51061 ggaaacgccg gaagcaatac agcaagagct gctgcaacgc attgacaagg tttttgattt
    51121 ttttctgaac cagtaagaaa ccagaacatg cacacacaaa aaaaccgctt gccagcgttc
    51181 gcctggtcgg gttacagttt tcccgcacct cataaaacgg gtgccgggat tgcaaccccc
    51241 gctgataacc aaagcgcaca accgcgccag cggttttttt gtgcgtaccg tatcgccata
    51301 tctttttcgc acacgaatta tggtggggcg tatggggccg acttcggtcg ggccgggttc
    51361 tttggttacc ggtggttgca accctgtacg tctcaccacc cagaattgca acctctggat
    51421 ggtgagtttt caaaattcac aaccaaagag gccacaccat ggcaaaccgc aagcaacagc
    51481 gcgcccgcgc tgagcgtctt cacatccaca ccgaaatcaa ccgcagactc catcgggctg
    51541 catctgtctc cttcatcatg cagtcaaacg tattacaccg acttaacgac ggtatcgctg
    51601 ccgggtatta cgcggcagtc ttcagctatc tggccgaaga cctggcagaa ctgcaggacc
    51661 ttatcaacca gcaacaccac cactaaacaa catcctgaag ccatcccgca cccgtgtgcg
    51721 ggaggcttcc gcacgtctgt taccggaggt tctcatgaag agcgttaagg gactttcaga
    51781 tattcaggac ttttttaact ggatgtgggc agtggatccc cggctggctt cccgcctgaa
    51841 cgactggcac gactattacc gccgcgcctg taaacgggcc gccagacgcg tcaccacata
    51901 cggggatttc tccttcaccg ttgacgaccg ttaccgcgtg gacgtgaaag gtgatggtga
    51961 tggttttggt ttatttcaca tcaacggaac cgacagcacc gccatcagct ggtcgtcgtc
    52021 agagatgaca cttgttctgg aaatgacaac gcacagcgtg gccgtgaaag gcatatacac
    52081 cgcagaagac tttatcggtg aatatcagcg ccttctcgtg ctgtaccgcg cggcgtttca
    52141 ggcggcgact gccggggagt gcgacgcatg agtgaccaca atgcacagct ggcgtacccg
    52201 tggaacgccc cgctaccggt tatcgatcct gaaacgttcg gcagagcgca gccaaccgca
    52261 ttacgccagg ccattcagag ctacatcaac gaggatattc gcactgacgc cagactggat
    52321 gaagaaaccg tcgccttcct gacaaccaca gacgaaggga agcgaattaa ttcgcatctt
    52381 catcacgacg aagagcgtcg gatcagactg gaaaaactga cgcgacacaa acgcgaaaat
    52441 ccgcccacac tggtatctga agccatggcg gagctgcgcg ccctgccgcc gtttcttgcc
    52501 ggtccgctca tccgggacct ggcacgcctg agccgcaaac aggagagcgc acgccgggaa
    52561 gggcttaaga acaacagcgc atacgcggcg gataacttcg ttcgtcacgg gctgcgtgcc
    52621 cgcctgaaac gtatcacccg tgtgaatgaa cgctttgcca ccccggcctt taaggccacc
    52681 gccgcgcgcg aacggctgga tgaactgctt atgctgccgc agctgagccg tgacgaaatc
    52741 cagcgccttg ccaccctgac cgccaccgcg ttttccgccg cactggagcg cgcatgtgat
    52801 gaggtcatcg aaaagaccgg caggggtgac gatgacctgt tcacctggtt actgacctac
    52861 cagccactgg cacacatggc gttaaaactg ggtatcacgc caccctgctg gccgtcgctg
    52921 gaaatcaggc ccgaccgccg caccgccccc gacccggaac aggtgccggg tgcggtcatg
    52981 cgcctttcct gtgcgacatg gtggaacagt cagttaagac acctccggga catctggcgt
    53041 gaagagctgt tacgcgccgc aggcctggtg tcacgtaaga cgtcggtata tatcagccat
    53101 gaagcgttac tggactggcg cgaaaaacgc cagcgcaccc gcgattttct gaaggcctgg
    53161 gacatcgaga acgaagaagg agaacggttc agccttgagg atatctactg ggcaggtctc
    53221 tctaatccgc gtaaccgccg taacgagatg atggcctgcg tccggggaat ggagcaggtc
    53281 gccgaagcac gcggcgacgt ggcgtttttc gtcaccgtca cagccccttc ccgttttcac
    53341 agcgtgaacg atgacggcag cctgaacccg aaatactgcg gagcaacggt acgcgatgcc
    53401 agtgattatc tggtttaccg ctttttcgcc gccgtccgca ggaccatcga caaggccggg
    53461 ctgggctggt acggcgtgcg caccgtggag ccccaccacg acggtacacc gcactggcac
    53521 atgctggtat tcaccagcca ggaaaacgaa gcccgtatca ctgaaatcat gcgcaacgcc
    53581 gccatcaggg aggaccgcgc agagctgggt gatgatatct ccccgcgttt taaatgcgaa
    53641 aaaatcgacc cggcaaaagg cacaccggca agctatatcg cgacctatat cggtaagaac
    53701 ctggacgcca gcgccttcat ggggaatgac ccgaaaaccg gcaaacctta cgtcgataag
    53761 gaatcaggca aaaccatggc cgaaaccgtg gagaacgcca tcggctgggc aggccttcac
    53821 cgcatccacc agttccagtt cttcggtatc cctccccgcc aggtatggcg cgaactccgc
    53881 cgtcttgccg ggcaaatggc ccgtaacccg gctgccccgc agcgtctgga ccatgacgac
    53941 attgacgcca tactggccgc cgccgacgtg ggatgttttg ccacctatat cacccgccag
    54001 ggtggtgtgc tcattccccg caacacgtac ctggtccgca ccgcatacga aactgccgaa
    54061 gaggccaacg actacggcga attcccgcaa cgcatttacg gcgtccgcgc cccgtcactc
    54121 ggggagcgct acaccatatg cacccacccg gacacctgga aactggtcag acgtaagccg
    54181 gaaaacgaag accgcacgga cgagggtttt gacgttgagt gcggcttttc cgcaccttgg
    54241 actcgtggca ataactgtcc ccctgaccag aatctgacca taaacgaccc gccagaaagg
    54301 cagccggacg gacgcaggga gcagagactt accctgccgg aaggtgattt gggtgaatgg
    54361 ttaaggtcac tgacccccac agagcgaaaa caactcacca ggcagctgaa aaacccgcca
    54421 cctgacacgg aaaccgtgaa aaacggcggc atcagtgcgc ccgctggcgg cgttcgcgtg
    54481 agtgtcgcac tgaccgctga gccacacatc accgcacaaa tcacccgcga gcttgatgcg
    54541 ctgggcgtgc agctgcatga aagcgccgtc gtttccgtcc gtcacggtgc ccgcgtgcgc
    54601 ctggctgacg gacgcaccgc gtactggaat gacagcaccc gccgcattga tatcaccccc
    54661 gcaccgaata cggaggcgcc acaatgaccc gcttttattg cgccatgcag cgccagcgtt
    54721 ttgacgaaat gatgcgccag cgcacgccct tactgaccct gaacaacatt gccacggggc
    54781 cacaaccggg cgatgagctg gaaatcacgg tcgtcagtcc cgatcgcacc accacggcca
    54841 tcatcaccga catactcaac ggacgaccgg acagcaccaa ccgcagctgg cttgtcgccg
    54901 ttcgtccgcg ccgtccgccc ggagagccgg agccgctcac gcctgccgga aaaatcatgc
    54961 ggcagtacca cgaagagcag gcacacaaac acaacgcaga actcaccaga acactggtca
    55021 caattaaccg caaagcggca attgcccgct atgtgcattc agccgctggc agacaatcac
    55081 gcaggtaaac aaagaccatg aacataacca gaaacagcca actaaaggag cgcaccatga
    55141 acagcaccag gaggatcagc gacagagagc ttacgaaaat gattgagtcg gcgcagcagc
    55201 tgctttcacg acaaaacccg gtcgtggaag aaacgtggtg ggagaattta cttgcagcac
    55261 tgaccgaact ccgggaacgc cggaacaacg aaggagccgc caaatgaaga ccccaggaat
    55321 gaccggaaag gaaatcattt tacgctacct ggaaacgcac acacgtttta gcgccccaga
    55381 actggtagaa aaactgggcg tgaaaagaac cgtcattaat caggccgccg gaaaaatggt
    55441 acgggaaggg ttgctggttg tggacgggta cacggacaag ataacgcatt accggaagcc
    55501 aacggaaaag gaacgacagg aactggcgca acgggctgaa cggcagcagg aaaaatcagt
    55561 catcgaagcc tgcaagcgca gcgaaatcat gaagcgaatt ctgtttatct acggcgctgc
    55621 attccgttaa aggagctggt gagtcggggc aaaaattctt tttgccccgg cgaagcagca
    55681 gacaagcgaa gcgcgtcagg aacgccgcga cagaagttca agcgcatacc ggcgatcttc
    55741 cgggcgcatg gcatcaacaa gaaacttcac catattgcga ccggagcggg cgccggggct
    55801 gagggtatgg gaaaaggtca tcgtacagac gaaggtgtgc ccgcattcga catcactgca
    55861 tgcacagtac agatccgcaa gttcaggggt tttccagtct gtcttccgga tgacagcagg
    55921 tgcgccacag tcaggacacc gggctttaaa aatccgcatc ttacctcccg ataacacgta
    55981 tcccggggaa gacattttac ccgaccggca ccgctttttc ttccgccaca tgcccggcat
    56041 ccacatcaaa acaaagataa aggtgttccg gtatatcggg atcgctgttc acggcgttca
    56101 tgattttacg ctgaagcggt atcacctcat ccttgcggta cgtttcacgc gtcgtttccg
    56161 ggttagggat ggtggccccg ttcgtgggga tgatccccgc aagaccggca ggaaagcggt
    56221 gtgccgtaaa cacatcctgg gcgctgatcc ccttgatgtt tgaaaattca tccttggcgc
    56281 tcacctcacc aatcggaata attttcaccc catccggcga acctttgggg atgttgatgt
    56341 acagattgcg gaaatttccc agccccttag actccgccac cttctcgcgg atctcatcct
    56401 ccatctccgt ggtgatccca gggtcgttcg tatacagcaa aaagcccata tgcgccccgt
    56461 tgttgtagta acggcgacga aaaaggctgg cctcgctgtt cagcagcgcc gagtgaatgc
    56521 caccgatata atccggcagc ccgtaaattt gctgacgcgg atcatacatc cgcagaaaga
    56581 taatatcttc cggcgtatag accagcgcgg ggccttcctg aagcaccacg aactcaccgg
    56641 ttttgcggcg ccggagatac agcgacggaa gcggctcaag tgccacgacg tcgccgaata
    56701 cgttacgcac cttgagcagg gcgagatcgc cgaacaacag gtagtcaaat accgcctgtt
    56761 ccatctggtc cgttgtgaga ccaccaccgg tatagccccc cgccaccatg ttacggcggg
    56821 catacagcac cccaccgtgc tgggcgttaa ggttcgggag ctgcgccagt gccaggcggt
    56881 taatcggcag ctgccagtgg ttgtgttcgt tgtcatacca gatgttatgg tagtccgtgc
    56941 cggtggtcag caccggttcc ggctgaccaa acgtgattgt gctgccacgt gcgggcgtga
    57001 atgctttttt cgtcacgcgt gacgttttgt ttctgcgttt cttgctcatg cggctttccc
    57061 gaaggcccat gttgacgggc gttcatagtc ataatctaca ggctcattaa tcacggcgtg
    57121 cgaaatggca aaaaacacat ccgcatgccc cgtaacatcc gaccgttccg ccacgaacgt
    57181 cagcgcgtta ccgctttttg tggtggtgcg gcgtatggac atgaacgacg cggggatctc
    57241 cgtgcgttca cggctggcct catcaatggc gtctttcgcc cactcgatac gcttatgggc
    57301 caccacgtct atcattttca tcaccaggcg gtttttgctc tccacgctgt aaagaatggc
    57361 gttagcctca cgtggcgcaa agcgactgac gatatcaaac acccctttcc cgataccggt
    57421 gatatctatg ccgatatagg taatgttaaa gcgctgcatc agttcgcgga tctgatcggc
    57481 ctgccaggtg aagttaaatc cctgccactg ccagacggcg agcacacgga accgctcgcc
    57541 ttcatggaca ggtggagcca caatcacaaa cgtggaatta tccccggacc ttgacgggtc
    57601 aaagcccgcc cacacctcac gattgccgaa cggacgtgcc gccgtgggat cgtagtcacc
    57661 ccacgtctcc atctccacct cgcacctgac cagttcggag aacttaaata ccgcatcctt
    57721 gctgtcaaca aactcgcagc cgtacagcat cgcataggtg gtcgggttgt agaggttcct
    57781 gatacgttca atatcaaccc gtgcagacag gccacccgcc accgcctctt ccagccggat
    57841 aatgtagcgc cagatcccgt cagggcattc acagcccaca cgcatcgcac tttctttcgg
    57901 aaactcaacc gcacgacgcg ccggatcatc gccgcgccac tcgtcaccgt tccacaccag
    57961 ataagcctgg tgggttttgg cgctgggcgt ggaaaagtac gtcgtgcggt atttgtcgtg
    58021 ggtcgccatt gccgaggcca cttcatgcag tcgcgtgaat tttgggatcc agaacacctc
    58081 atcgccgtaa aggtgaccgt taaatccctg ggcggtactg gcattggtgg acaggaaacg
    58141 cagcattgca ccattgctca gacggatatg cttacccgtg agcgtgatcc cgaactgctg
    58201 ggcgaaatca ataatatatt cacggaagat ttccgcctgt acccgtgacg cagaaaagaa
    58261 aatctggtta tcaccggtca ccaccgcatc ttcgaacgct tcccatgcaa aataccaggt
    58321 catgcctatc tggcgactct tgaggatgaa acgccgctca cggtgccggt tctcgcggca
    58381 gtgcagctgg taggcataaa gatttttacg cgcccatgca tccagcattt cagcagtcag
    58441 cccggaaacg tcgtttttcc ggtagcgccg ctttttccct tcacccccgc cgctggtcgc
    58501 cgccacaccg tcgtcgtaca ctgccggacg cgtggcggca atttccgcca tcctttcagc
    58561 atgtttgttt ttctgggcga tgatcttcac gtgctgtgcg ataagttcac gcagttcggc
    58621 aagttcacac tctgtcttgc cctcccgtcc ggtcagcaca tcaatacgcc gggcgatcac
    58681 gtcctcaacg gattcagcgg gcagcagggc ggcccactgc cccacatcac accagtggta
    58741 aaccgtgcgc ggcggaatac cgagttccgc cgcaatatcg cgggggtgcc accgcttcag
    58801 gtaaagcgat cgcgccgcct ctttgatttc gtcagaatat ttagccatgc gcccattatg
    58861 gcggcgcatc cggcccgcat tcaggcagaa aatttcggct gtgttcggct atcccgcgtt
    58921 atccgaagac agccgaattc agccagtagc cccccgctgt aaaaatcccg acactcaccg
    58981 gagttcttca gattttcacg taaaagaggc cacgatgccg cagccaacgt tgatgaccga
    59041 ctggatatgt atcgccacat caggaccgac cgttgacggc cgcgaaattg atccgcagtg
    59101 gctgactgat gccgctgaaa cctattcccg caacacctac acggcgatgc tgtggccctg
    59161 gcatgaggaa gaaatcggac accgccagta caccagtaat ctgggtgaag tggactcact
    59221 gaaagcggaa accggcgagg acggtaaaac ccgcctctac gcacggctgg tccctaacca
    59281 gtacctgatt gaagccaaca gactggggca gaagctgttc acatccgccg agatagtgga
    59341 gaacttcgcc cggagcggac gcgactatct ccttggcgtg gcggtgacgg atatccccgc
    59401 cagtctgggg acggaaaaaa tacgcttcac cctgaacaac gaggaataca cggcacagcg
    59461 ggcgaactgc cagacgttca cgctgggcag cctgtcaccg gacacctccc cggccccccg
    59521 taaacctgaa tcattcctga aacgcctgtt cagcgcagga cagaccgaca acaccgatac
    59581 cagtaagcct gatacaggcg aggacgcaaa aatggacgaa attaaagcac tgcttgaaaa
    59641 gctgatcacc ctggtggaag gcggcagcgc cgctgccact ggcgagggca cagcagacac
    59701 gccggaagaa gcaaaagccg aagtcgcgga aatcgccgcc gaaatcgccg acgccgccgc
    59761 agaggtggca gagctggcag aagaagtgaa agccgccccg gaagacgagg ttaaggccga
    59821 agcctttacc gctgcaaaag cgaagctgga agacgccatc aaagccttca gcaccatcac
    59881 accggaaaaa ggcgaacgtt accgccgctt tgccgcccgt cgccgggacg acaccgacaa
    59941 agaagaaatc aaagctattg ccgaaaaact taacgacctg accacgaagc tgtcagccac
    60001 cggcatcacg ccacgaccgg ctggcacacc ggcagaaagc gaaaaaccgt ttgactttgt
    60061 ttaaggacac gcaacatgca actgaaccag aaaaccgaat cactgcttcg taagtacgct
    60121 gccggactgg gcgcggcaaa cggggttgac gacgtcacac gctattttgc gctgtctgac
    60181 ccgaaggaga ccgcgctgcg tgacgccatc atgcactccg acgagttttt atcaaaactg
    60241 ccgaacgtgc tggatgttga acaggtgaca ggccaggtgg ttaccaccgg cgttccgggc
    60301 ctccataccg gacgtaaaaa aaatgcccgc ttcacccttc cgctgagtgt taccgggaat
    60361 gaatacaagc tggctgaaac cgattccggt gcccacctcc tgtacaccac actggtcacc
    60421 tgggcaaacg caggcggtga aaacgagttc tttaaccgca ttcagaattt tgttaacaga
    60481 cagatcgcgc tggatatcct gcgcatcgcc tttaacggta ccagcgtggc cgaagatacc
    60541 gacccggtaa ccaacaaaaa cggcgaggac gtcaacaccg gctggcatgc catcgtcaaa
    60601 ggccgcagcg caaaacagat cgtggaggct ggcccgacgc tgggcaaggg tggcgacttt
    60661 atcgggctgg atgcagccgt ggcagacctc gtgcacaccc tcattgatga gtcgtatcgc
    60721 ggtcatccgg atctggtggt actggttgcg cccgatcttg tggcacagga caaggtgacg
    60781 ctgcttaacg ccatcgacgc ccccacggaa aaagtggccg cgcagcttat cggtcgccag
    60841 atcgccgggc tgacagcata caccccgccg ttcatgccgg aaggacgcct gattgtcacc
    60901 acgctggcaa accttcacat ctacacgcag cgcggcaccc gtcagcgccg tgcggagtgg
    60961 aacgacgatc gcaagcgttt tgaaaacaac tatctgcgca tggaaggtta cgccgtggag
    61021 cacgacgaaa tttacgcggc ctacgacaag ctgaccatca agggcgcaga agcggcagaa
    61081 gagcccgcac ctggcggcgc ataagcacag agggcggcgc aatgacaccc tgccagcgac
    61141 accgggcgaa aatccgcaca caggaggcac tcgaacgccg ggaagcgctg acggcctccc
    61201 cggtcagctt ccacctgtta cgcgcggagc ttgatcgcga tgtggcacgg ctgcgcagtc
    61261 ttcctgtccg cgaagagcgg ctggcgttca agcgcgacat tctcctgccg cgctggctgc
    61321 ccgtcgcgga gcgctatatc gctgacggaa agcgccacgc ctgcccggta ctggtgtact
    61381 gcatcatctg gctgttcgac acgggcgacc tgagtcgcgc ccttgactgg gcagacattg
    61441 ccatcagtga aggccaggcc acgccggaca acttccgcag caccctgccc gccttcgtgg
    61501 ccgacacggt gttgcagtgg gccaccgaca acagcgaggc cggacacagc atagagccgt
    61561 atttttcgcg caccttcagg aacgtcacca ccacgtggcg gctgcatgag gaaatccgcg
    61621 cgaagtggtt cagatttgcc gggctgtatc ttctgcgcga cgaggcaggc aaaccgagag
    61681 ccaccgcgat taatgatact gccgtactgg aaaaggcaga cgcatacctg gcacaggcgg
    61741 cagcactgag ccgtaccgct ggcgtaaaaa gtatcagggc gcgcatcagg gcacgcatca
    61801 gcgcccttaa tgcagcgtaa acgactaccc gcagggccgg gcgggcgcgg gaggaggcat
    61861 cagtgacgga aactgtatgg ccgtggaatc ccggacagcc cgccttttta ttcatatgag
    61921 gcacgtcatg agcgaaccga gcttcagcgt cagcggtaaa ccgctgaaat ccacaccgca
    61981 gacggtgaca aacggcgagc cgttctggcc ggaccttgat ctggctgagc ttcaggggtc
    62041 acgcacgctt ccggctgatt tacccgacga cacggcggcc atggcgctgc ttgccgcagt
    62101 ggccgaggtg aacaccacgc tggcgccggt tgtagcgcac tggaaggcgc aggggcatga
    62161 acgcgccgca gacgtaccag gggccaggct gggcgatgaa acccagctgg tcgcacagta
    62221 caaaaaggcc gtttatgccc gtgccaaggc cgatcttatg ggcgagttcg ccaccattgg
    62281 tcgccgtgaa acctatccgg ggcaggaaag cgaggacaca cgcgccacct tactggctga
    62341 agcctcgctg gtcatgcgta acatgctggg gcacagacgc gcgggagtgc acatcatatg
    62401 aaccagctgg aaagcctgac cgccttcatt accgaaaatc tgccgcctga cgccatgcag
    62461 ttcttcatga gcagtcagga agacgcgcag cttatccgca gtcagaaggc gctgggtaac
    62521 gaccagatcc gcatgggcgt gatgacgtac accggcgtga ttgcatggga taacttcccg
    62581 ttccgcaaat actcaccggg gctggtttac gcgctggtca tggcctggct ggaagagcac
    62641 gcggacccgc tgagggagga attccgcccg gaggatgtga acgtcgaccc gcagtttaac
    62701 gatgaggact cgtgccttct cgatatcacg gtgggactgg ctgacccgct gatcatccgg
    62761 gtgcaggaag acggccccgt tccgtacagg gggaaacgct ggacactggt taaccctgaa
    62821 atctggacgg cggaaaccat ggatctgttt gtcagcagga gccgcgaatg ataagcggcc
    62881 agctgaaccc gcagcagctg gcagaagcgc aggcgatgct tcagtcgctg gatctgccac
    62941 caaaaaaacg ccgtcgcctg ttatggcgta ttgcccggca cggcattatc gccgctgcca
    63001 gacgccatca gcgcgatcag gccacaccgg acggtacagc atgggcaccc aggaaacgcg
    63061 gtaaggggaa aatgttcaga aagctgccgc gtcttctggc ggtccgtgag atgcccggac
    63121 aggatgccgt aaaggtttat ttccgtaacg gcaatcacac cagtggcagg agccgcatca
    63181 gtgccggtgc cgtgggctgg gttcaccaca acggcgcaga aatccgggtc agtgctgaca
    63241 gcttcagcgg tcaggaccag agcggacaga ccgccacccg ccgacaggca aaacgactgc
    63301 gggagctggg ctttaaggta cgtcagaagg ggcgctggcg gaaagcatcc atgaaatacc
    63361 tgttaacgct ttcgcgggcg caggccgggt tgctgatcac cgttatggaa gatcagatta
    63421 tcgccggacc acgtcagcag aaggcccgga catggaccat tacccttccg tcgcgcgtct
    63481 ttctgggggt aacaaacgaa gaattttcac acatcctggc acgccagatg aacgcaatgg
    63541 gctacggcct ggacgttgac gcacaggata tcagataagg agtcacaccg tgagctggcc
    63601 gaatgtaacc gtaagccatc agaaccgttt caacggtgcc acccgtgaag tggagcgcac
    63661 gctgcttttt gtgggctatg gcaaaaagaa cacaggtaac accctttccg tctcccctga
    63721 gaccgacctg gacgacgtgc tgggaccgga tgaaagcctg cttaaatcca cgcttaccgc
    63781 cgcaatcgcc aacggtggcc agaactggtt tgcctatgtt cacgttctgt ctgaacccaa
    63841 acccccggca ccggagggcg gcgacgctaa cgccgcatgg gtggatgcgg tgaaaaaagc
    63901 gcagaccatc gcctctgccg agggcgtggt gattgccatt gacatcacgg ccagagatgc
    63961 cgttaaccgc gccaccgaaa cccgcgccct gcttcagtcc gcttatggcc gttttgtctg
    64021 gttcatgctg tgcgtggccg gaccgggcaa ggatgaggca tgggcggcat acgtcacccg
    64081 catcagtgcc atacaggacg gcgtggcagc acctggcgtg atggtggttc cccgcttatg
    64141 gggaaatgag cccggcgtac tggcgggccg tctctgtaat ccttccgtca ccgttgcgga
    64201 cagtccggca cgtgtggcaa ccggtgccct ggtggcgatg ggtaacgatg agatcccgca
    64261 ggacggcaag aaacagcccc ttgagctggc cacgctgcgc tcgctggaag gcaaacgctt
    64321 cagcgtgccc atgtggtatc acgatttcga cgggctctac tggtcagacg gacgcaccct
    64381 tgatgtggag gggggagatt accaggtcat cgaaaacgtg cgcgtggtgg ataaagtctc
    64441 ccgccgtgta cgtctgcgcg ccattgcccg ccttgcggat cgcgcactca acagcacacc
    64501 gggcagcatt gccgccagcc agacatattt cagccgcacg ctgcgtgaaa tggcgctcgc
    64561 cacggagatt aacggcgtac cgttccccgg cgaggttaaa ccgccacagg acggggatat
    64621 caccatcgtc tggaccagca gtacacacgt gcagatttac attgtggtac gcccctacga
    64681 gagcgcaaaa gccattggcg taagtattga actcgacaca gcactggaga tctgacatgg
    64741 ccggaaatga acgtatcagc ggcggcgcgt ttgatatcaa ctacgacggg atcatgatcc
    64801 acgtggaaag cgccaccgtc accatcaccg acaacagcgc cgtggcacag acgcgcggcg
    64861 tgccgaacgg ctacacccgt ggttctgttt ctgccgatgt ggaggtggaa gtcgacgcgc
    64921 agaactttaa aaagtttggt gccgtggccc gctctgcggg gtcatggcgt gccattcctg
    64981 aaaaggattt tctcttttat gccaacaacg gcagcgagga ggaaaaaatc gaggtgttcg
    65041 gctgcgtccc catgttgtcg gacgtggtga acatcagcgc aggcgaagcc ggaaagacca
    65101 cgaaaaaaat caagttcatg gtcacaagcc cggactttat cagcattgac ggcgtgccct
    65161 acctgtcagc ccgtgacacc cgcgatctga aggggtgatg ccatgccgga cggtcagact
    65221 tcactcctgg caaaactgat tatcatcggt gctgccatcg ggctggggcg tctcatggtg
    65281 agccgcgatc gcatcacagc ccgtctgctt atcgggcgaa tgattatggg ctctgccgtg
    65341 gcgccggttg ccgccattcc gctgtttaaa tttccggaca tgccggaact ggttgtggtc
    65401 ggtctggcgt gcgcgctggg cattatgggc agcgccttta ttgaagaatg cttctgccgt
    65461 ctggcagacc gctacctgaa gcagaaggaa cgcagaaatg acccataagc cttacatttc
    65521 gcctttcctg agcctgtatc acgcgcaggc gcatgacgtt ctgcgaatgc tgccggaaaa
    65581 cagtgtggac gcgctcatca cagacccgcc atacagttca ggtgccacac acaaggcggg
    65641 gcggacatgc cagggcagcc acgccaaata cctcaacgat gaagatcttc accgctttga
    65701 aggcttcgaa ggcgagaaca tggacgcccg ttcatgggaa tactggacgc atctgtggat
    65761 gacacaggca tggcgcacgg tacgacccgg tggttacgcc ctggtcttca ccgactggcg
    65821 gcagttgccc gcgctgacgg acgcattcca ggccgccggt tttacctggc gggggatcgt
    65881 ggcatggaac aagggacggg gctcaagaac accacatacc ggttattttc gtcaccagtg
    65941 cgaatatgtg gtctggggca gcaaaggcgc actgggcaaa tccccttcag ggccgttcga
    66001 cggctgcatc acgcaccaca cacgcccaca ggagaaacag cacccgacag gtaaaccgga
    66061 atcgttgatg gatgagcttg tgcgcgtgtg tgaacccgga gggctgatcc tcgatccgtt
    66121 tatggggtcc ggcaccacgg gcgtggccgc ccttcgccgg ggctgccgtt ttacgggtat
    66181 tgaactgaac gcccactatt tcagcgtggc acaggcgcgc cttacgggga cggaacagag
    66241 gagtgactga tgaaactgag cgaaaaacaa caactgtttg ccgtcatgat tgccgatctc
    66301 atccactggg cacaggagca cggctatcgc ctgaccttcg gcgaggcata ccgcacgccg
    66361 gagcaggccg cactgaatgc gaaaagcgga aaaggcattc gcaacagcct tcacacgctg
    66421 cgcctcgcgg tggacttcaa cctgttcatt aacggcaaat accaggccga caccgacgcc
    66481 tacaggccgc tgggggaata ctgggaatcc atcggcggca catggggcgg acgcttcagc
    66541 cgggcagacg gcaatcattt cagccttgag cacaacggga tgaaatgatg aaacagctgg
    66601 cgggctgcct ggtgatcctc tttgccgcca tgtgggcagg ctggctggcc catgactggc
    66661 atgacgccag ggtggcactt gccgaacagg aagccatgga agccacgcga cgggcggtga
    66721 cagaagccgg gcagcagtcc gcccgcatac tggaaaaacg gctggaggcg ttaaagggca
    66781 atgaaattca cactgaacgc gttattcaca cggaaaccgt ccgcccggta ttccgcaatg
    66841 tgtgcgctac tgatgattac gtccggctgt tcaacgcccg tgctgaagcc gctgaacgta
    66901 ccctggcagg aaaaccagct gacggaatgc ccgaagacac tgccgagact ggcaggcccg
    66961 acgggcgctg attttgatgc ggcactgcga accctcacga gccgctacac cgaatgcgcc
    67021 gccaggcata acagcctggt ggaaaccata cgaaacacgg agaaactgca atgaccacga
    67081 agaacaccgg caccatcatc ctgtccgttg cgggcgtaac cctgagcttt gaaccgaaca
    67141 aaacggcatt taacaacctc atcaacgaaa tcacgatgac caacaaggtg gcgccgatga
    67201 gcacctatct taaccgcatc gtgacgcctg ccagccgcga cgcactggct gacctgattg
    67261 cgcgttatcc gggctgtgag atgcagatcg tcgagaagat taacgaggtt tactccccga
    67321 aactggagat tgagttaaaa aactgaccgc gcgggtcgaa agcatccgcc gcaatcccct
    67381 tgagcagtat ctggcattgc ggcgtcacta cctccccgga gagccggacg acgaagagag
    67441 catcgcccgt gccatctggc tggatgaata ttttgcccgt acccgcgcca acaagaccgc
    67501 tgaaggtata gcgatcgcat ttaacggaag gtgacatggc cagccagctg gatttcatta
    67561 tcaatctgac agaccagctt acccgcccgc tgcggcaggt tcaggaatcc gtgacgggat
    67621 tcgccgaacg ctcgcagggc gcgttcgcgc gtatcggtgt gggtgtggcc ggactgtggg
    67681 gcgtggccga aggtatccgc gccgcgatgg atccggcgtg ggagatgcag aaggcgctgg
    67741 gcgagcttga ggccaaaggc gtcggggcgc aggcgctgac cgccctcacc acgcaggcca
    67801 tgaaactggg gaccgcattc ggtcagtctt ccacggagat catccgttcc gcatggcata
    67861 ttcgcggcgc actggacgga ctgagcgacg gggatttacc ccgtgtgacg gcagcaacca
    67921 gcaccctggc cgtggctgcc cgcgtcagca gcgaagaggc gagcggctac cttgccgcca
    67981 tgggtgcccg tttccgcagt gagttttctg ccatgggtgc cgtggggttt gctgaagatc
    68041 tggcaggtaa aaccgcgtgg atggttcagc agttcggcgt ggaaatgagc accatgcagg
    68101 agcttattga gggcaccaaa aacgcgggcg cggattttgg cgtcagcatg gcagagcagt
    68161 ttgccatact gggcaccctg tcacggacca tgggcaccga ggcatcgggc atctatgagg
    68221 aattcctgcg cagtgccccc gctgccgcac aaaaaatggg gatgagtttt gttgatgcca
    68281 ccgggaagat tttacccatg ggtgacatcc tcgaccgtct gcaggcgagg ttcggcagga
    68341 acatcgaggg taacgtggcg gcacaacagg cgctggatga cgccttcggc agtggcgcgg
    68401 acgtgattaa aaaactctgg ggacagcagg aaatgcttaa ccggagcata tcagcgctgg
    68461 ggcgtaatga cggcatgaag cgtgccacgg aaatggcgga agccatgact gacccgtggg
    68521 accgccttgt ggcctccttc tacaacatcc gggccgccat cggtaacgcc ctctacccca
    68581 ttctcgcacc actggctggc cgttttgccg atatcggcgc gacgttcgtg cgctggctga
    68641 acatgttccc gaacatcgcc cgctggctgg gttacatcac gctgaccatg ctggcgctgg
    68701 gcgcagccgg tgcggtcacg aacatcgtgc tgggtgtgtt cagcttcatc atggaaggcc
    68761 ataaggccat cataaagggc gccagggcag cctggagtct gtacacgctt gcactcacaa
    68821 aagtacgcag tgccatcatt gccaccagtc ttgccgcgcg tctggcgggc gtgtcgtttc
    68881 tgaccatgag cgccccggtc ctcctggttg ccgccgccat cgctgctctg gtggtggtgg
    68941 ccattaagtt ctggcagccc gtcaaagcct ttattcgcgg ctttattgat ggttttacca
    69001 gcgcactgga cgccatgagc ccggtgactg ccgcgttcgg tgacattgcc cgggcgctgg
    69061 ccccggtatg gcagggcata aaaacactgt tcagtgcctt ctccgatctg tttgcaccgg
    69121 tacagctgac cacgggacag atgaacagcg tcacccgtgc cggggaaaca tgcggtcgcg
    69181 tggtggccgc agcggtaagc ctgatatccc ccgcgctgga ggtggttatc agcgtgatca
    69241 gaaccctgat cgacatcttc gccatcgtca tcagagggtg gcaggacgtg gtagctgcat
    69301 ttgacccgac cgccccggtc gaatccttcc ggaaaatggc ggggatcatc ggtaacgtct
    69361 tcggggactt atggaacgtc attaaaaact cgtttaaccg gacgtggaac tggcttgcgg
    69421 aaaagctgaa caaaatcccc ggtatcagta tcgacatgaa gcccacggag caggtgaaca
    69481 ccatgacgcg gggcgcaaca ggcagcaccg tacctccgca gattgcagaa accgccgtcc
    69541 cggcactggc aggcaaccgc atcagcgcag agctgccacc cggaggcata cgcaaccaga
    69601 tacgcacgga cacctcaacc cgtatcgaca acagccggac gttcggcaat ctgacaatca
    69661 acacgcagaa cccgatgacg ccggggcaac tggatgaatg gacggagctt tacgcgggat
    69721 gaacacgcca tcgctttaca tcgacatgct tatcactgac ggcagtttta cgctgaacag
    69781 cggcaacgag ccacagcgat gtgataaccg ggtcagcatc ggccaggacg tggtgcacag
    69841 cattatcgaa agcgggatcg ccgccctgct tatcggcgaa cgcagcccga cgctgcgcag
    69901 cgacatcatt acccggctta tcctcctggt tgaggatgat gaacggctgg ttccggggac
    69961 ggtgttcatt aacgaagagg caccgggcag gctgcatgtg acggcagaga cacaggattt
    70021 tggacgcata aacaccaggg tgaactatga aagggaaacc ggacgttgat tttaatcagg
    70081 tattccgtga aagcggtatg ccggaaacag aagaagagat cgccaccctc tttcatgagg
    70141 atgtacagaa aaccggactc atcaccaaca cctcgcccat gagtcccttc tggcggctca
    70201 ttctgaccat tgtcatacga cccgttcagt ggctgcgtga tgcgctggtt gacgttgtgc
    70261 tgcccggtat gtttgtcgcc acggctgacc ggtcacttct tctgcttcat gcgcacgacg
    70321 tcaacatcac cccaaaaccg gccagtaagg ccagtggcgt gatctggttt gccaaaacca
    70381 gggcaaatga tgatgttctc atccccgaag ggaccatcgt ccagaccgcc agaattaacg
    70441 gcgtgatata cagcatggcc gtcacggagg aaacgcttat cagcagcggt aaaagcggcg
    70501 catccgttcc cgtggaagcc accaccgaag gcagcgggca caacctcgcg ccggggtact
    70561 tccgtatcct tcccgttgcc attcccggca ttgcatccgt ggtgaaccgg gaagactggc
    70621 tgaccgtacc gggtgccgat gaggaaagcg acgacgaact gcgcgacaga atacgcaacc
    70681 agtttaacct cgtgagcagt taccacacgg acgccgtcta ccggggactt atcgccagcg
    70741 tggcaggcct gagcatcgac cggatctact ttcagcacga cgccccgaga ggaccgggaa
    70801 cggccaacgc ctacctgtta ctggacagcg gcgaggcctc tgcccctttc attgaagcag
    70861 tcaaccgcta catcaacgaa gaggggcacc acggacacgg ggatgatttg cagtgtttcg
    70921 ccatgccgga gacgcgtcac cgtctggacg tcaccgtata catcagaagc tcaaccaccc
    70981 tgccaccgga cggcgcggaa gggctgaaac aggatatcag cgacctgata cgctgcgctt
    71041 ttcgggagaa cgcccgcttt aatgtgaaaa aagtctggcc ctattcgcgc ttttcgtttt
    71101 ccacgctggg caaggaaatt catcagacct tcccggtggt ggcttcggtt cagttttcac
    71161 tggaagacat catcagcgat ctggccgtgc cacggctcga atccctgaca gtggaggtgg
    71221 tcattgaatg actttttgca gcgacttcgc tcgctgaaac tgccctcctg gatggacagg
    71281 ggggaacccg acaaactgct gcgggccgcg caggcgttct ggtcccgcgt atgggaatgg
    71341 atcacctggc ccgtgcgcca gtttgacccc atgacatgtg cagagcccgt acttgattta
    71401 ctggcatggg agcgggacat cacccgtttt aaaggtgaac ccacatcact gtaccgccgc
    71461 cgtatcgccc acgcctttgt caatgcacag gacgccggag aggtggcagg atttattgcc
    71521 atttttgaac ggctgggcat tggacatgtg gagttgcagg agcgcaagcc cggcgtggac
    71581 tgggatgtga tcctggtgca ggtgacagac agccagatag ccgacaacag cgatcttctt
    71641 ctggagatca tccgcaaata tggcaggaca tgccgccgtt accgttttga ggtggaaacc
    71701 cttaacccct tcatcatcca ttatggcgca tacgaagggg agtatatctg ctggacggcc
    71761 agggcagagt gaatgactga aggagtatga ctttatggcg aaatctgtaa ttaccagcgc
    71821 ctttatccgg ctcaaggcac agcaggcggc ggaaggcacc cccgttgtgc tggatgagtt
    71881 tgtttttgca aacgtcccgg acctcaaccc ggaaacaccg gtatcccccg acgaaaccct
    71941 gccacctgaa gatcagattg ttcaccgcca ggccgttggc aaaaccggcg tggtgaatga
    72001 gaatgccgtg gtttattcgg tcaccctggg ggcagagctg ggtgattttg aatttaactg
    72061 ggtgggactg gtcagcaaag caaccagcac gctggccatg attgttcacg cccccacaca
    72121 gaaaaaaatc agaacccaca acggtaacga aggtaatgtg ctggtccgtt ccatgctcat
    72181 ggagtacagc ggtgcgaagg aagccacagg tatcaccacc ccggcgcaga cctggcagat
    72241 agacttcacg gcacgcctgg catccatgga cgaacgccag cgccgcgaaa acgtcgatat
    72301 ttacggtaac gctgcattct ttggggacgg ctggctggtt aaaaaaggcg aaagatggtc
    72361 actctgcaaa gggaccggtt acgtggaggg attacgcgca gagctgaccg aagacatacc
    72421 gctgaccacg ccagacttcc cgtcccgtat ctggctgaca gtatggtgga ccggtacgct
    72481 caccagtgaa tggacggtgg agcacgatat tatcatggcc gaccccgccg ccacggaaga
    72541 gatcctgctg gaagcacaca ggaaggaggc cgcccggcaa tatttttttg cccttgctga
    72601 cattgatgaa gacggcaacg tgactgacct gcgccccaga ggaacgccgg aagaacgccg
    72661 ctggctggac gctctgaaaa aacacgagaa atcacgcaac cacccggacg caacgctgaa
    72721 agaaaagggc ttcacacagt taagcagcgc caccaccagt acatcagaga cacaggcagc
    72781 cacgccaaag gctataaaga tcgccatgga caacgccgat ggtcggcttg cgaaaaaccg
    72841 caatggtgag gacattccca acaaggagct gttcctgatc aacgtgggcg caccacgcgt
    72901 ttatgcaaaa gacgggtacg tgggtgaagc agggcaacgc tggacaacag aacaattcgt
    72961 ggaatggctg aatgaacgtg gcgccttcag accaggactc tggatattca aagccaatat
    73021 ttcactttcc cggcacagcg tgctcactga tacgggctat ggtgatatct gccttggagg
    73081 ctgccttatc gaagtatcag gcgacatcaa cggggggatc atcaggatca cgacaggtct
    73141 cgaaagccca aaccctgccg acatgtacct ccgcacacag ttcaccgggg ttgttttaaa
    73201 taaagggaaa agcaccatct ggtaccgcac ttacacggac aaaaacaaac ccacaccaca
    73261 ggacatcggc gcactgcctg caagtgagtt gttacccgca ggcgttcccc tgccgtggcc
    73321 gtcagacacc ccgcctgacg gttacgccat catggtgggg cagacattcg ataaagcgca
    73381 atatcccctg ctggcaaaag cctatccgga cggtcgcatc ccggatatgc gcggctggac
    73441 aatcaagggg aaacctgcca gcggtcgtgc ggtattgtca caggaacagg acggcatcaa
    73501 atcgcacacc cacggaggga gcgtctcaaa tactgacctt ggcacaaaaa cgacctcatc
    73561 cttcaattac ggcacaaaaa caacaaacag cgcggggccg ttcagcgcaa caatatcatt
    73621 aagaaccgcc accgcgtcac cggaaggcag cggtgactgg accgcatacg ggccgggctc
    73681 cggggcgaac ctgatcgcca gagtttcgga acacacacat tcggtaagca ttggcgaaca
    73741 cacccacacg ctcgcactgg gttcacatgg tcacggcctg accattaacc ccaccggtaa
    73801 tgccgaaaac accgtcaaaa acatcgcatt taactacatc gtgaggcttg cataatgacc
    73861 tttaaaatga gcgaacaccc gcagacaatt aaaatctaca acctgtcagc cgggacaaat
    73921 gaatttatcg gcgaaggcga tgcctggatc cctccgcata ccggactgcc tgcgcactca
    73981 acggacattg ccccgcctga cattcctgaa ggacacgtgg ctatctttga tgaggatgaa
    74041 aacgcctggc acctcatgga agatcaccgg ggtaaagccg tctggcgtct ggagtcgggg
    74101 agcgccttac tgattactga gccgggcccg ttaccggaaa acgtgacaga aataaaaccg
    74161 gacggggagt tctgccggtg ggatggttca tcatgggtta aggatgaagc agcagaacag
    74221 gcttaccggg tgagcgaggc ggagaacaaa aaacgggaat taatacagga agcaacaaat
    74281 acaataaaca tcctccagga tgccgtggat ttaaatatgg cgaccgacga tgaaagcgcc
    74341 ctgcttcccc tgtggaaaaa ataccgggta ttactgaacc ggacagacac gggcaacgcg
    74401 ccggatattg tctggcctga atccccttcg ccggatcatc acgcaggctg accggaggca
    74461 ccatgacgtg gacaagaaaa acaatcagcg tcagcgctga catgcccccg ctgagctgtt
    74521 ccgtcatccc cgtcacaccg tggacttacg ggctgggacg atatgaagaa tcgggggtat
    74581 atttaagccc tccgaacgcc ataaactggc tggccgggaa aatggccgga agccgtgcat
    74641 ccggcgacgt gaccattatc atgattgccg aaaacaccca cgatcttttt atacagtcaa
    74701 tggcggcact gaccgccgtt ctgcccgtgc cggtattcac gcaggcacag cgcatggcgc
    74761 aggctgccgc cagtctgagc actgacaaaa tgcaaatccc gatcaccacc gacaccctgc
    74821 ccgcacctgt tcatttttcc gttgccacaa tacgcagcgc agtcagtgcc gcccgtgcag
    74881 cacaggccag gcagctggca gcacaacgcc cggatgccgc agggcttcgc cgccagataa
    74941 gcagctttat cagcagacgg gctggcgcac ttgccggact ggaagagggg atcagaacac
    75001 tggcagaaca gaaggcgcag gcctgggtat ttcagtaccg ggggtatcac cgcgccgcag
    75061 cggcggccat ggtgaaggat atcccggcac cgaccgccgt acatactgtg gccgcactcc
    75121 tggcggcaga ctcattaacc gaactgggga aaatgatcca tgagccagac cgcactgctc
    75181 gcccttgatg gtgagggtat tttcatgcag aacatggtga tttcgccctc gatgcagttc
    75241 caggaaaagg accagtccgg tcagacatcg agcaccgcga acgccgagca gggcatcaag
    75301 gccaaagagc ttcgcgtaac cgggatgatc acctttgaca atgagcgggc ccttcagcgc
    75361 attttccagc tggcctctgc caccaccggg gacggtgccc tgaaagttta ccgtatcgca
    75421 aacgccaccg ctgccgccat taactttcgt gaagggacat tttccgggca gatagacgca
    75481 cagcagcaga ccgaccgcct cgcctggcag gtcagcttca cattacgcga aagacgaagc
    75541 gtaccggaaa aacgccaggc acgcgccgca cccgccagca gccgtaaaca gacacctcag
    75601 acgcccggcg caaaaggcaa aaccgtggcc gacgaaacac cggagaaaat gacctggttc
    75661 gaagaaaagg tcctcaaacc cgtcaatgat gcgctggagt aaccatgaac cccactaaac
    75721 gtctgttact gggtaacgac accattcagc tggtcagttg ttccgtcatg cttgagctga
    75781 acgcgtgcgg acgcggattt atcacggcca gaacagagca gaactacacc gggcgccccg
    75841 ttcgtctcga tatcggatac ggaaacgacc tggtgcgctg gtttaccgga tacgttgaac
    75901 gcagccagcc cgctgaaaac ggctcggtgc ggcttctgat tcgtgaaatg gcggggatcc
    75961 ttgatcaccc cttcccctgt tcatttcagc acccgacaat gcgcacggtc acacagtggc
    76021 tgagtgagac cagcgggctg gaaatcatcc tgccggacaa cgcggcttac actgacagac
    76081 ccgttccgca tttcacacac agcggcacgg gctacgaact gctggccagt ctggggcgta
    76141 ttttctccgt accggattac atctggcatc cgttaccgga cggcggcatt atggccgctg
    76201 ctgccgcgca ggggatgttt tccggtcgcc ccgtcaccat cccgcacgag ttcagccagg
    76261 catcaacggg cgggcacagt atgaccctcc cgatgattca gacgctgcga ccgggtgtgg
    76321 aggtgaacgg tcagcgcctg acgatggtca tgctggaaga tgacaacatg accatcacgt
    76381 ggacaccgcg caataaagcc accggcgccc ccctccagaa gtcccctttc cgtcgccagg
    76441 ctgaaaaggc cttccctgaa ctggcatcag ggcttcacct gcccaaactg gcacgtgttg
    76501 aggcgccgac cgaagccgtc agcgcgggca atattgccga cccgttcaga ccccgctacg
    76561 cggtgaatct ccagctgctt gacgagaacg gcaacccggc agcggatacg cccgtttata
    76621 acgccgttcc cctccccgta cccatggcgg gcagtgagtc cggcatgttc cagttcccgc
    76681 cacccggaac cctggtggaa gtgggatttg tggaaggccg ggcggataag cccttcatcc
    76741 gccagataat ggcagagggc cacaatctgc ccgacgtgaa gccgggcgag cagttgcagc
    76801 aacagcgcga cggcgtatcg cagcgcgtga cggtcgccgg agactgggag cgccagacag
    76861 accagcggat acaggagaca tcacgcgagc gcgtggtgag cgcagacgag gaaacgcgga
    76921 gcctgaccag ccgggagacc accatacagg caaccgacac aaccaccgtc ctgggcgtat
    76981 ccacgcttca ggctggcgcg gtcacccata tcactgaaga gaactacagc atcagcacgg
    77041 gcgggcacat gatggtggtt gccggtacct gtgagcgcga cgtggaaggc gacacactgg
    77101 acacagtggg cggcagcctt accgaaaaga ttaagggcgt ccgtcacagt attgcgctgg
    77161 cgcagaagct ggttggcgat acggtgaagc tgggaaacga gggcattaat gtactgacca
    77221 tgctgacgga ccttgccgat gtggtggaag agctggccga tattacagca tcacataccc
    77281 accccaaaac gggcacgtct ccgcaggccg cacagttcag ccaggtcgct caggagtgcc
    77341 gccagcttaa aaataaatat tcaccaataa ttgaataatc attcacttta acaggccgcg
    77401 caatgtggcc ttttttattc ccggacaata aaaaatattc atcaccggac atcacccacc
    77461 acgaaaaaac cgtccgcagc agaaaaaaag atggacgaaa cgccccactt aatccccacg
    77521 taaaacgtcg cgtaacgcca cgtaacgaag agataacccc gccagcccga ttcgatcccc
    77581 tgacatcaga aaacgatttc tgcgccccaa aatcgccgca aatggccgga aattttcggg
    77641 ggcgacgtaa cgcgtaaagt gacgtaatcg gcgctacccc gcacacgcct ccggtatttg
    77701 tgtacgataa attttgcaaa tcgaaagggg tgcaaaacac ccagccaggg cgcgccatta
    77761 ctgaggtttt cccttcagaa tggttttgca ctttgtgcaa ggttttgcaa aagtgtgcaa
    77821 aaactccggc tttctgagga tcccgaaaaa ttttaatgca ctgaataaca agagattttt
    77881 aaaattacgt cacgatcgaa gatctgaaaa aggatccgac gacgaaaacg aaaaaatcag
    77941 gaaagccaga actggcgcgg cctggcgggg agtggcagca aaaatcactt ttgcacactt
    78001 ttgcacaact gctccgaaaa aggatggcga aggggaagga aaaacagcca cgtcatccgt
    78061 tgcacgtaac ggacgtaaaa ggtaaattac gcgcgggtgc ctttcggctg acggctggaa
    78121 ggattacctg aaggccggat gtggaaaggc cccgagtcaa acatttgttt aacccgaggc
    78181 cctaacccat ctacctcgca agtggaaggt tagcgcctcc ctgtaaaagg agtcaagcgc
    78241 tatgccgcaa aaaacgatca tagtgagcat tttatgcata actattttga tgattgtctg
    78301 gatgatacac aagtcaccgt gtgagttcag gcttaacatc atgtggtcac aatttgcggc
    78361 gttcttgcag tgtaaacagt aagaaaccgc agcgggggtg aaagcccccg ctatccggtt
    78421 gccgggttcg gtcgatatgg caccctttct ccgttgaatt acatagacat ttgcccgcga
    78481 tatgcgggct atatttttat tttttgaata ggttaataaa ttcaggtttt ttcttatctc
    78541 ctgcttccct tcgtttaacc caacaacacc ctgactgaag taaacagaca aattcatcct
    78601 gcttcagctc taaggtatct ttaagtttta attgtttttt cgcagcatca ggaacaaaaa
    78661 catcaacaat aatcttcccg gggtcataca gttgagtttc aatcagcaca ggtaatttat
    78721 ggacaggtct gggttccata cgagaaaaag aaaactcacc atccatcaca ccatcaaaag
    78781 tacttacaga atcgtaacca gaaaagcact catatttcac ctgaagaaca acagcatcat
    78841 caagctttaa aaaatcgcaa taagaatcat acgccaggcg atcactataa aaaacatcac
    78901 cgcgattaaa ctgaaaggaa tccccttgcg tccaaatttc ataattaaaa aactctgttt
    78961 ttactggggt aagttttata tccaaagaat ccttgtaaca ctcttttaca gcaaagcgcc
    79021 aaccagctgc gtaaccatca acaaataccg caatcaacgc attactacgg cgaaactccc
    79081 ttacagtacg gatagaaagc gagaactcat ctatcatact cttgtaagtg ttaacatcct
    79141 tctcaagcga tttactgata tcattctcaa ccacgcagtc ttgctcaaga ggaatttcac
    79201 cagtatcaat aaaattgata aattgttctt ctgtcagaat aattacgcca taatcgctgg
    79261 ctttctgaac tttccgccac cctttgttct ttcctacaca aaggaaaaac aaactttttc
    79321 caacatcaga cttaacatca ataccatttt cttttgcaag agcacttaag cgaga
//
LOCUS       DAZFFI010000010        74058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFFI010000010 DAZFFI010000000
VERSION     DAZFFI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.010"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..605
                     /locus_tag="ACHJ12_000886"
                     /pseudo
     CDS             2..605
                     /locus_tag="ACHJ12_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            708..1211
                     /gene="yfbM"
                     /locus_tag="ACHJ12_000887"
     CDS             708..1211
                     /gene="yfbM"
                     /locus_tag="ACHJ12_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4988716.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1278..2735)
                     /gene="nuoN"
                     /locus_tag="ACHJ12_000888"
     CDS             complement(1278..2735)
                     /gene="nuoN"
                     /locus_tag="ACHJ12_000888"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4988717.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAIGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2742..4271)
                     /gene="nuoM"
                     /locus_tag="ACHJ12_000889"
     CDS             complement(2742..4271)
                     /gene="nuoM"
                     /locus_tag="ACHJ12_000889"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4988718.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4435..6276)
                     /gene="nuoL"
                     /locus_tag="ACHJ12_000890"
     CDS             complement(4435..6276)
                     /gene="nuoL"
                     /locus_tag="ACHJ12_000890"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4988719.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6273..6575)
                     /gene="nuoK"
                     /locus_tag="ACHJ12_000891"
     CDS             complement(6273..6575)
                     /gene="nuoK"
                     /locus_tag="ACHJ12_000891"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4988720.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6572..7126)
                     /gene="nuoJ"
                     /locus_tag="ACHJ12_000892"
     CDS             complement(6572..7126)
                     /gene="nuoJ"
                     /locus_tag="ACHJ12_000892"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4988721.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7138..7680)
                     /gene="nuoI"
                     /locus_tag="ACHJ12_000893"
     CDS             complement(7138..7680)
                     /gene="nuoI"
                     /locus_tag="ACHJ12_000893"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4988722.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7695..8672)
                     /gene="nuoH"
                     /locus_tag="ACHJ12_000894"
     CDS             complement(7695..8672)
                     /gene="nuoH"
                     /locus_tag="ACHJ12_000894"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4988723.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8669..11395)
                     /gene="nuoG"
                     /locus_tag="ACHJ12_000895"
     CDS             complement(8669..11395)
                     /gene="nuoG"
                     /locus_tag="ACHJ12_000895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4988724.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11448..12785)
                     /gene="nuoF"
                     /locus_tag="ACHJ12_000896"
     CDS             complement(11448..12785)
                     /gene="nuoF"
                     /locus_tag="ACHJ12_000896"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4988725.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12782..13282)
                     /gene="nuoE"
                     /locus_tag="ACHJ12_000897"
     CDS             complement(12782..13282)
                     /gene="nuoE"
                     /locus_tag="ACHJ12_000897"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4988726.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13285..15087)
                     /gene="nuoC"
                     /locus_tag="ACHJ12_000898"
     CDS             complement(13285..15087)
                     /gene="nuoC"
                     /locus_tag="ACHJ12_000898"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4988727.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15181..15843)
                     /gene="nuoB"
                     /locus_tag="ACHJ12_000899"
     CDS             complement(15181..15843)
                     /gene="nuoB"
                     /locus_tag="ACHJ12_000899"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4988728.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15859..16302)
                     /gene="nuoA"
                     /locus_tag="ACHJ12_000900"
     CDS             complement(15859..16302)
                     /gene="nuoA"
                     /locus_tag="ACHJ12_000900"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4988729.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16932..17870)
                     /gene="lrhA"
                     /locus_tag="ACHJ12_000901"
     CDS             complement(16932..17870)
                     /gene="lrhA"
                     /locus_tag="ACHJ12_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4988730.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18790..20007
                     /gene="alaA"
                     /locus_tag="ACHJ12_000902"
     CDS             18790..20007
                     /gene="alaA"
                     /locus_tag="ACHJ12_000902"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4988731.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20091..20690
                     /gene="yfbR"
                     /locus_tag="ACHJ12_000903"
     CDS             20091..20690
                     /gene="yfbR"
                     /locus_tag="ACHJ12_000903"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4988732.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20749..22581)
                     /gene="yfbS"
                     /locus_tag="ACHJ12_000904"
     CDS             complement(20749..22581)
                     /gene="yfbS"
                     /locus_tag="ACHJ12_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4988733.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22668..23318)
                     /gene="hxpA"
                     /locus_tag="ACHJ12_000905"
     CDS             complement(22668..23318)
                     /gene="hxpA"
                     /locus_tag="ACHJ12_000905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4988734.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23329..23823)
                     /gene="yfbU"
                     /locus_tag="ACHJ12_000906"
     CDS             complement(23329..23823)
                     /gene="yfbU"
                     /locus_tag="ACHJ12_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4988735.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23906..24361)
                     /gene="yfbV"
                     /locus_tag="ACHJ12_000907"
     CDS             complement(23906..24361)
                     /gene="yfbV"
                     /locus_tag="ACHJ12_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4988736.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24699..25901
                     /gene="ackA"
                     /locus_tag="ACHJ12_000908"
     CDS             24699..25901
                     /gene="ackA"
                     /locus_tag="ACHJ12_000908"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4988737.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25976..28120
                     /gene="pta"
                     /locus_tag="ACHJ12_000909"
     CDS             25976..28120
                     /gene="pta"
                     /locus_tag="ACHJ12_000909"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4988738.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28310..29830
                     /gene="yfcC"
                     /locus_tag="ACHJ12_000910"
     CDS             28310..29830
                     /gene="yfcC"
                     /locus_tag="ACHJ12_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4988739.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29863..30405)
                     /gene="yfcD"
                     /locus_tag="ACHJ12_000911"
     CDS             complement(29863..30405)
                     /gene="yfcD"
                     /locus_tag="ACHJ12_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4988740.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30463..31014)
                     /gene="yfcE"
                     /locus_tag="ACHJ12_000912"
     CDS             complement(30463..31014)
                     /gene="yfcE"
                     /locus_tag="ACHJ12_000912"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4988741.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEFFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31070..31714)
                     /gene="yfcF"
                     /locus_tag="ACHJ12_000913"
     CDS             complement(31070..31714)
                     /gene="yfcF"
                     /locus_tag="ACHJ12_000913"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4988742.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31850..32497
                     /gene="yfcG"
                     /locus_tag="ACHJ12_000914"
     CDS             31850..32497
                     /gene="yfcG"
                     /locus_tag="ACHJ12_000914"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4988743.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32554..32916
                     /gene="folX"
                     /locus_tag="ACHJ12_000915"
     CDS             32554..32916
                     /gene="folX"
                     /locus_tag="ACHJ12_000915"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4988744.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32937..33830
                     /gene="yfcH"
                     /locus_tag="ACHJ12_000916"
     CDS             32937..33830
                     /gene="yfcH"
                     /locus_tag="ACHJ12_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4988745.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33878..34780)
                     /gene="rpnB"
                     /locus_tag="ACHJ12_000917"
     CDS             complement(33878..34780)
                     /gene="rpnB"
                     /locus_tag="ACHJ12_000917"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4988746.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(34977..35750)
                     /gene="hisP"
                     /locus_tag="ACHJ12_000918"
     CDS             complement(34977..35750)
                     /gene="hisP"
                     /locus_tag="ACHJ12_000918"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4988747.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35758..36474)
                     /gene="hisM"
                     /locus_tag="ACHJ12_000919"
     CDS             complement(35758..36474)
                     /gene="hisM"
                     /locus_tag="ACHJ12_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4988748.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36471..37157)
                     /gene="hisQ"
                     /locus_tag="ACHJ12_000920"
     CDS             complement(36471..37157)
                     /gene="hisQ"
                     /locus_tag="ACHJ12_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4988749.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37247..38029)
                     /gene="hisJ"
                     /locus_tag="ACHJ12_000921"
     CDS             complement(37247..38029)
                     /gene="hisJ"
                     /locus_tag="ACHJ12_000921"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4988750.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38250..39032)
                     /gene="argT"
                     /locus_tag="ACHJ12_000922"
     CDS             complement(38250..39032)
                     /gene="argT"
                     /locus_tag="ACHJ12_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4988751.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39298..39867)
                     /gene="ubiX"
                     /locus_tag="ACHJ12_000923"
     CDS             complement(39298..39867)
                     /gene="ubiX"
                     /locus_tag="ACHJ12_000923"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4988752.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADIETHLVMSQAARQTL
                     SLETGFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39962..41479)
                     /gene="purF"
                     /locus_tag="ACHJ12_000924"
     CDS             complement(39962..41479)
                     /gene="purF"
                     /locus_tag="ACHJ12_000924"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4988753.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41516..42004)
                     /gene="cvpA"
                     /locus_tag="ACHJ12_000925"
     CDS             complement(41516..42004)
                     /gene="cvpA"
                     /locus_tag="ACHJ12_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4988754.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42263..42925)
                     /gene="dedD"
                     /locus_tag="ACHJ12_000926"
     CDS             complement(42263..42925)
                     /gene="dedD"
                     /locus_tag="ACHJ12_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4988755.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42915..44183)
                     /gene="folC"
                     /locus_tag="ACHJ12_000927"
     CDS             complement(42915..44183)
                     /gene="folC"
                     /locus_tag="ACHJ12_000927"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4988756.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44253..45167)
                     /gene="accD"
                     /locus_tag="ACHJ12_000928"
     CDS             complement(44253..45167)
                     /gene="accD"
                     /locus_tag="ACHJ12_000928"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4988757.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45323..45982)
                     /gene="dedA"
                     /locus_tag="ACHJ12_000929"
     CDS             complement(45323..45982)
                     /gene="dedA"
                     /locus_tag="ACHJ12_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4988758.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46065..46877)
                     /gene="truA"
                     /locus_tag="ACHJ12_000930"
     CDS             complement(46065..46877)
                     /gene="truA"
                     /locus_tag="ACHJ12_000930"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4988759.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46877..47890)
                     /gene="usg"
                     /locus_tag="ACHJ12_000931"
     CDS             complement(46877..47890)
                     /gene="usg"
                     /locus_tag="ACHJ12_000931"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4988760.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47956..49113)
                     /gene="pdxB"
                     /locus_tag="ACHJ12_000932"
     CDS             complement(47956..49113)
                     /gene="pdxB"
                     /locus_tag="ACHJ12_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4988761.1"
                     /translation="MSETEKGLKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLAD
                     ADALMVRSVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAV
                     VEYVFSSLLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRG
                     DEGDFRSLDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAV
                     VDNTALLTCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQ
                     VFEAYSKFIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAQLR
                     KVAGIPGEFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            complement(49272..50276)
                     /locus_tag="ACHJ12_000933"
     CDS             complement(49272..50276)
                     /locus_tag="ACHJ12_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4988762.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFDKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWMYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSQKIADGLKPSSANRYMNRLSGMFTVLKKIGLWDAEHPVRGISILYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWSEACKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTRETGGLFEVKYREFCLALKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQKILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHAVSTGD
                     "
     gene            complement(50373..50702)
                     /locus_tag="ACHJ12_000934"
     CDS             complement(50373..50702)
                     /locus_tag="ACHJ12_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4988763.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEVGQISPALSLDGSCNSEGDRVSTE
                     TIQKLSR"
     gene            50807..51094
                     /locus_tag="ACHJ12_000935"
     CDS             50807..51094
                     /locus_tag="ACHJ12_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO4988764.1"
                     /translation="MSESELGGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNEGMRKAFFEQPKERRDAWLLWLGL"
     gene            51101..51307
                     /locus_tag="ACHJ12_000936"
     CDS             51101..51307
                     /locus_tag="ACHJ12_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988765.1"
                     /translation="MTSHIISAILTLVFIEIGVIEIYLFRKLTGHKERFIELSIEYIA
                     AYTKGLFPAAIGAMLIAFVIWFIG"
     gene            51560..51901
                     /locus_tag="ACHJ12_000937"
     CDS             51560..51901
                     /locus_tag="ACHJ12_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO4988766.1"
                     /translation="MKQQRNSRFRNGAERHANRFATSASRSNIRYSLSDTHATPDGYP
                     VKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALSSGDNQFGQDFTLYEALRTVDRLL
                     RGQSFIKQADL"
     gene            51912..52199
                     /locus_tag="ACHJ12_000938"
     CDS             51912..52199
                     /locus_tag="ACHJ12_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988767.1"
                     /translation="MTKDHTQGVFIRFIDFRGELLLRASAIDGVAPAGKNGAAEATYV
                     YLNGTRLIVELPYQTVREIISEAEKARQVNGDKPYIEIICMDSETEIQKAD"
     gene            52211..52453
                     /locus_tag="ACHJ12_000939"
     CDS             52211..52453
                     /locus_tag="ACHJ12_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4988768.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYELGNIA"
     gene            52450..52563
                     /locus_tag="ACHJ12_000940"
     CDS             52450..52563
                     /locus_tag="ACHJ12_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988769.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSIKKIERFFTGE"
     gene            52650..52853
                     /locus_tag="ACHJ12_000941"
     CDS             52650..52853
                     /locus_tag="ACHJ12_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988770.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVRVNLIRQVQESRR"
     gene            52850..53095
                     /locus_tag="ACHJ12_000942"
     CDS             52850..53095
                     /locus_tag="ACHJ12_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4988771.1"
                     /translation="MTILDYIAANPGCSGGEIAAALNTPTTAINAELRRLWRSGSVIR
                     KERKTGGRFSYQINPMPFGCGNPLTNTFNQLLKEARA"
     gene            53092..53391
                     /locus_tag="ACHJ12_000943"
     CDS             53092..53391
                     /locus_tag="ACHJ12_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988772.1"
                     /translation="MSAINHQELRELATDLQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTPLAIRLTLRHEIEDFCATLEAPGEPETPKAIQQELLQRIDKVFDFFLNH"
     gene            53403..53672
                     /locus_tag="ACHJ12_000944"
                     /pseudo
     CDS             53403..53672
                     /locus_tag="ACHJ12_000944"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53714..53944
                     /locus_tag="ACHJ12_000945"
     CDS             53714..53944
                     /locus_tag="ACHJ12_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4988773.1"
                     /translation="MANRKPHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHELQQLIQQQNKLH"
     gene            54017..54382
                     /locus_tag="ACHJ12_000946"
     CDS             54017..54382
                     /locus_tag="ACHJ12_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988774.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            54389..57154
                     /locus_tag="ACHJ12_000947"
     CDS             54389..57154
                     /locus_tag="ACHJ12_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4988775.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYACGTLRKIFERLDRTDHRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNAPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPDEIE
                     TIVSHVCDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFASYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRELMRALRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQRFEIVRKPDTRPDSRITQLWQRLSRNHGVNSTEIRHNPVSSYLKQLGASD
                     PEAAARLASTIQQDQNTMKTPVTVLSDMLRAIRDAEHAQRINETTERARHKASLLQDK
                     GNSEKKK"
     gene            57335..58456
                     /locus_tag="ACHJ12_000948"
     CDS             57335..58456
                     /locus_tag="ACHJ12_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4988776.1"
                     /translation="MGDLGGIQSDLAQLVRLVIAEQYDDVRLYVARLVRKYRETMPAL
                     SEQLDLYLRNKPQKPAQSLRKVTAPKQALPQTMPVDEESRLTLLKAPNEKIVSKPLLS
                     SQIEDSLDQIILERKHIDRLKALGLQPTRSAIFVGPPGVGKTLTASWLAQKLGVPFYV
                     LDLTAVMSSYLGKSGNNLRAALDFAKKGPCVLLLDEIDSIAKKRSDDSDVGELKRLVT
                     VILQEVDEWPSSSLLLAATNFAELIDPALWRRFDLVLNFEKPDSESIKEAIKRFSGPD
                     YAIFARWIDLLAIMFKNESFSNIERSINKFRRSVALGISSDEELIGSFIKDGLSELDR
                     NERKEIAVTLKRNSKLSQHAISDLTGVSRDTIRKYSQKM"
     gene            58480..60705
                     /locus_tag="ACHJ12_000949"
     CDS             58480..60705
                     /locus_tag="ACHJ12_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFO4988777.1"
                     /translation="MAKTNFLIGRGELLTSDIPGPKRVPGKAEVYSLYESKQRLLPQI
                     ASTIENFDDLPSQACPYDYAVAKLTLNPSYIARSFFPAKMLKSTGLTSIGSRTVKITP
                     QSWKRKVTVSEVTTTQMFVAGKRESFKELPHWVSGLKEFSDEAIDFARIERFDAYLPE
                     ERIITIGEQDSSYFEVGVHLLPDNEQDLVQQQFVQFASNLNIKVHTSLAFTAGTLWFV
                     PIQADRITISKLAKFTFVRVIRPVPRLRGIRPIQRNYGPLAQCKLPAEGPVSSEIKVA
                     ILDGGLPSEHAISPWLKSYRVLDDHAEDDPDGLEHGLAVSSAFLFGPISNKSEAKRPY
                     SYIDNLRVLDNKTCQEDPLELYRTLGFIEEVLLSRQYQFINLSLGPDLPIEDTEVHAW
                     TSVIDDLLSDGETLMTVAVGNNGEMDRKSGNARIQVPADCVNALAVGACDTVNDTEWK
                     RAPYSAIGPGRSPGVMKPDLVAFGGDTDEYFHVLGKGKKPVIIPQLGTSFASPYALRA
                     AVGVRAILGNDLSLLAIKALLIHASKQLDHPCTEVGWGKLPEDLNDIIVSPDGVARIV
                     YQGELKPGKYLRASLPIPEGGLKGRINLAATFCYATTTDPQDPASYTKAGLEVTFRPN
                     DSKIKDGKQNAETKGFFELAKYSTESERRSDMGKWETVLHNSKNMLGSTLKNPVFDIH
                     YNAREAGASIASHKAEKIKYALIITIKASKHQDLYNEILRTYNQVLVPIQPQTSIPIR
                     S"
     gene            61274..61798
                     /locus_tag="ACHJ12_000950"
     CDS             61274..61798
                     /locus_tag="ACHJ12_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988778.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVHLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLETTEGFRRMPLSQIPAATPKARGGNDERKLIFSIETV
                     VDDTPGEVAFVHTPLSIAMYNDEVQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            complement(61813..62859)
                     /locus_tag="ACHJ12_000951"
     CDS             complement(61813..62859)
                     /locus_tag="ACHJ12_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4988779.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(62859..64610)
                     /locus_tag="ACHJ12_000952"
     CDS             complement(62859..64610)
                     /locus_tag="ACHJ12_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO4988780.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLERHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAASEVDVELKGGDKI
                     ILSNGAELHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYVYWNGDRWNEKKAAHKRQHFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            64765..65601
                     /locus_tag="ACHJ12_000953"
     CDS             64765..65601
                     /locus_tag="ACHJ12_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO4988781.1"
                     /translation="MANEKKTSRKKFRVAVSGVTADGREINGDMLKAAATSYNPSVYG
                     ARVNIEHILSPLPGSEFSAMGDVVGLSTEDITNGPLAGRTALYAEIEPTARMMSLLND
                     GKKIYSSIELEPQSTITGGPYLRGLAMTDTPASLGTERLAFAAQQRMQLMTFNCQQGD
                     VAMFTAAMESELIELTEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            65625..66677
                     /locus_tag="ACHJ12_000954"
     CDS             65625..66677
                     /locus_tag="ACHJ12_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO4988782.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            66723..67523
                     /gene="gpM"
                     /locus_tag="ACHJ12_000955"
     CDS             66723..67523
                     /gene="gpM"
                     /locus_tag="ACHJ12_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO4988783.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLTDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSADASEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            67625..68119
                     /locus_tag="ACHJ12_000956"
     CDS             67625..68119
                     /locus_tag="ACHJ12_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO4988784.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVTHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            68119..68319
                     /locus_tag="ACHJ12_000957"
     CDS             68119..68319
                     /locus_tag="ACHJ12_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO4988785.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            68322..68645
                     /locus_tag="ACHJ12_000958"
     CDS             68322..68645
                     /locus_tag="ACHJ12_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4988786.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            68642..69034
                     /locus_tag="ACHJ12_000959"
     CDS             68642..69034
                     /locus_tag="ACHJ12_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO4988787.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            69031..69438
                     /locus_tag="ACHJ12_000960"
     CDS             69031..69438
                     /locus_tag="ACHJ12_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO4988788.1"
                     /translation="MKLWPTLGVAFLLIAGWGTSMRLSWSLGRENARNEAQASTLKRT
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADVDHTGNASPLP"
     gene            69576..70043
                     /locus_tag="ACHJ12_000961"
     CDS             69576..70043
                     /locus_tag="ACHJ12_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4988789.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLTPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            70036..70671
                     /locus_tag="ACHJ12_000962"
     CDS             70036..70671
                     /locus_tag="ACHJ12_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4988790.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRFAEAVR"
     gene            70683..71249
                     /locus_tag="ACHJ12_000963"
     CDS             70683..71249
                     /locus_tag="ACHJ12_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4988791.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFSY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKGGKMSGNITHSGG
                     DFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            71246..71596
                     /locus_tag="ACHJ12_000964"
     CDS             71246..71596
                     /locus_tag="ACHJ12_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO4988792.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSARDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            71600..72496
                     /locus_tag="ACHJ12_000965"
     CDS             71600..72496
                     /locus_tag="ACHJ12_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4988793.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEITEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            72489..73019
                     /locus_tag="ACHJ12_000966"
     CDS             72489..73019
                     /locus_tag="ACHJ12_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO4988794.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            73022..>74058
                     /locus_tag="ACHJ12_000967"
     CDS             73022..>74058
                     /locus_tag="ACHJ12_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4988795.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQPRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTARGFVQLSSDTNSVSETQAATPKAVKAAYDLANGKYTAQDATT
                     ARKGLVQLSSVTNSTSEALAATPKAVKTAYDLANGKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGG
                     AGWFKLATVTMP"
BASE COUNT        18715 a        20045 c        18592 g        16706 t
ORIGIN      
        1 tatttctgat ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc
       61 aacgccccga cacgtcagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
      121 tgtgcatcca cgtaatgccg acaatattga taatctgaat aggtccgtag agtatataaa
      181 agagttcttt gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt
      241 acgactctgc gcctggtagc caggattacc cttacccgca atgtcctggc tttatcccga
      301 tcggggggac tttattgcct ggtcggcaga atacaggata tcaacgccgt tcgtcaggta
      361 aaaacggcat tgttatcatc ccgggattta tctgtttatt ctatgaatgc cccatggttt
      421 attcccggta ttgatttctc tgaccacctg aattattggc aacacgatat tcccgccgta
      481 atgattactg acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgatacagca
      541 gacagattga attatcagaa aatggctcag atggtgttat aaccttgtta cacaacagta
      601 aataataaaa atttctcgag gcgggtccag aaacagcgac atgtacattt tattttccct
      661 cgtctgtttt aagatgcgaa acataaaatt caatggagga aaaataaatg ggaatgattg
      721 gttattttgc agaaattgac tcagaaaaga ttaaccaact gcttgagtct actgaaaaac
      781 ctttgatgga taatatacat gacacgcttt caggattacg ccgactggat attgataaac
      841 gctgggattt tttacacttt gggctgacag gcacctctgc ttttgatccc gcgaagaatg
      901 atccgctgag tcgtgccgtg cttggtgaac acagcctcga agatggcata gatggcttcc
      961 tgggattgac ctggaatcag gagttagccg cgacaatcga ccgactggaa tcactggatc
     1021 gaagtgagct gcgtaaacag ttttccatta agcgactcaa tgaaatggag atttatccgg
     1081 gagtgacctt ttcagaagag ttagaggggc aactctttgc ttcgatcatg cttgatatgg
     1141 aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta
     1201 ttgttgggtg atggtgtcag ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg
     1261 acttacaaac tgatagctta catcagcggc attgctaacc gcacaatgct aatcagcggt
     1321 tgtggccata cacccagcac cagtaccaac agtgcagaga tcagcaccac gataccgcct
     1381 gcgctgtact gccagtttga cggtgcatcg cgacccggtt gttccggggc gtgcaggtac
     1441 aggctcaccg ccacgcgcag gtagtagtaa aggccgattg ccgaaccgac aaccacggcg
     1501 cccaccagcc accacaagtg tgcctggaca ccgatcgcca gcacgtagaa cttaccaata
     1561 aagcccagcg tcatcggaat accggccaga gacagcatca tcaccgtcat caccgccgcg
     1621 aggatcgggc gatgccagaa cagaccgcgg taagagaaca gggaatcagc atccgggcca
     1681 cggtacgggc tggacatcag gctgaccacg ccgaacgcgc cgaggctgct gaacagataa
     1741 ccggccaggt aaactcctac cgcttccatc gacatctcgc ccgtttgcag cgcaatcagc
     1801 gccaccagca gatagccgag gtgagagata gatgagtaac cgagcagacg tttgatattg
     1861 gtctggctca gcgccatcag gttaccgaag atgatagagg caaaggcgat aatcgccagc
     1921 actacccgaa tcgcttcgct gtcacccacc ggtgcgtaga ggaacagacg catcaccaca
     1981 ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc
     2041 tggtatacgt ctggcgtcca caggtggaac ggcaccagag agagtttgaa gccaaggcca
     2101 acaatcatca ggccgaaacc tgccagcaac agcggctcgt tgagcatacc gtcgccgagg
     2161 tttttaccca gcgcgacaaa cgacaggtcg ccagactgcg catacaccag cgccataccg
     2221 aacagcagga aagaagacgc tgcggcagaa aggatagtgt atttgatact ggcttccagc
     2281 gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca gcggcaaaga gatcagttcg
     2341 ataccgagga acagagacgc cagatggttg gcattcgcca gcaggatccc gcccagcgcg
     2401 gcaattaaca ccaacaggta gaactcatcc ttgttgtcgt tatagccttc aagccacggg
     2461 taggcgaaag tacaggtggc gaggctcgcc aacaatacca gcccggtgta aagcatggcg
     2521 aaaccatcaa cgcgcatcag cggcgtaacg tccatagcgc ccgcctggcc aacaaaccag
     2581 agcgaaacca gcgccgcgtt aagcccaata accgagagcg tagcgttgag gaaatgattg
     2641 cgtcgccacg caatggagag catcacaacc accaccgtca agccgacgat cagcaacggt
     2701 agcagtgcga tcaggttttg tggagttatt gtcatggcga tttacggcct tgtagtagta
     2761 acggaattaa caaaccactg ctggatattg ccaatcgcgg agtgcgaggt atccagaatc
     2821 ggctgcggat agaagcccag cagtaccagc agcaccacca gcaacaggat cataaacagc
     2881 tcacgcagcg acatccctgg cagttcctgg ctggcaatct ggcttttcgc tttaccgaag
     2941 taagcgcgat gtaacatcgc cagcgaataa acagatgcaa agaccagccc aaaggtagag
     3001 ataacggtaa tcaccgggac aacctggaag ctgccgaaca gaatcataaa ttcgccgacg
     3061 aagttaccgg tgccaggcat cccaagcgtt gccaccgcaa agaacagcga cagtgctggc
     3121 agccatttca tcttgctcca cagaccgccc atcatgcgca tgtcgcgggt atggatacgt
     3181 tcataaagct gaccacaaag aataaacaga cccgccgccg acaaaccgtg cgcaatcatc
     3241 tggattaccg cgccctggta ggccaactgg ctgccggtgt agatagcaat cagcacgaag
     3301 cccatgtggg aaaccgaggt gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc
     3361 atccacgcac cgtagaagat gccgataaca cccagccaca tagcaattgg cgcgaactct
     3421 gccgacgcgt tcgggaacag cggcagggag aaacgcagca aaccgtaagc ggcagttttc
     3481 agcaagatcc ccgcgaggtc aacggaaccg gcggtcggag cctgggagtg cgcatccggc
     3541 agccagccat gcagcggaac caccggcatt ttgactgcga aggcgatgaa gaagcccagc
     3601 attaacaggt attccacacc actggacatt ggcgtattca gcagctcttc atagttgaag
     3661 gtccagacgc cggtcgcatt gtagtgaaca aaaaccagcg ccaggatggc aatcaacatc
     3721 accaggccac tcgcctgggt gtaaatgaag aacttggttg ccgccgtgat acgcgtttta
     3781 ccgtcagagg ctttatgccc ccacagtgcg atcaggaagt acatcggcac cagcatcatt
     3841 tcccagaaga agaagaacag gaacatgtcg atggcaagga acacgccgat aacgccgccc
     3901 aggatccaca tcaggttgag gtggaagaag ccctgatatt tttcgatctc tttccacgaa
     3961 cagagtaccg ccagcacacc gagcagaccg gtcagcacga ccatcagcag cgacagcccg
     4021 tcaatggcga gatgaataga gataccaaaa cgcgggatcc acggcatgtc gaattcagac
     4081 tgccactgcg gaattccggc ggattgcgtc agtgaataac cgccctgcaa ccacagttgc
     4141 agcgacagcg ccagcgtcaa tcccatggtg atcagcgcga tccagcgtgg caccttgacg
     4201 ccaaagcgtt cggtctgcca gcacaggaag ccgccaataa agggaattaa tattagccag
     4261 ggtagtaaca tggcgatctt tattccttgt aaaagtcccg tcaggaccgg attttcaacg
     4321 aattctcacg acagaaaatt ctcttcggac tgggggtagt gtcggatgca ccgcgaaggc
     4381 ggcttatccg acctacgttc ttatatgccc aggggcaatc ccacaatcct taactcaacg
     4441 cagtaccatc aacagtgcca gcaccacgac cgcaccgatg ctcatggatg ccacatacca
     4501 gcgcagatag ccgttctcac ttaacagcag acctttacct gcaaagcggg aaaggacagc
     4561 cgggatgttc atcattgagt tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa
     4621 cggcttgacg aacactttgt catacagcca gtcaaatccc caggcgttgt accaccaggt
     4681 gcccagcaga cggcccggcg cactgttggc gatggaggtc accagagtac gtttacccag
     4741 ccacagccag gctgccagca gaatgccgac caccgcgacc acgccagagg taatttccag
     4801 ggtcaacatg ctgccgtgcg ccagttccgt cgtttgcgga agcacgccct gcagcggcgg
     4861 tacaatcagt gcgccaacga aggtggaaag gatcagcagc acaatcagcg gcaggctgtg
     4921 agttacccct ttcacggcgt gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat
     4981 catacggaag gtgtagagcg aggtcataaa cgcaccgacc agacctgcca ccatcagatt
     5041 gatatgacca ttcgccatcg cacccgcgag gatctcatcc ttactgaaga agcccgcagt
     5101 gaccagcggt agtgccgaca gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg
     5161 aatagattta cgcagaccgc ccatcttgaa gatgttctgt tcgtgatggc aggccagaat
     5221 gacggaaccg gatgccagga acagcagcgc tttaaagaac gcgtgagtca tcaagtggaa
     5281 aatcgccgca tcccatgcct gcacgccaag cgcgaggaac atgtagccaa tctggctcat
     5341 ggtagagtaa gcgagaacac gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag
     5401 cagcagcgta accgccccga caatacccac cagatgcaga acttccggcg tcatcaggaa
     5461 caggccgtgg gtacgggcga tcaggtagac acccgcggtt accatcgttg cggcgtggat
     5521 cagcgcggag acaggcgtcg ggcccgccat cgcgtcagca agccatgtct gcaacggcaa
     5581 ctgcgcagat ttaccgaccg caccgcccag cagcatcagc gtcgcccaca tcagcatgtt
     5641 attgccgtca gcaaagtgcg ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc
     5701 cagttcgttg taaagaatga acagtgcgaa agcgaggaaa acgtcaccca cacgggtcac
     5761 gacgaacgct ttcattgccg ctgcgccatt cttcggatcg gtgtaataga aaccgatcag
     5821 cagataggag cacaggccca cgccttccca gccgaggtac atcagcagca ggttatcggc
     5881 gagaaccaga actaccatgc tggcgatgaa caggttggtg taggcgaaga agcgagagta
     5941 gccctcttca ccgcgcatgt accaggaggc gtacatgtga ataaggaaac ccacaccagt
     6001 gaccaccgag agcatggtca gcgacaggcc gtccagcacc aggttaaaac cgatgttaaa
     6061 gtcgcctacc gacatccacg tccacagcgg ctggctgtat gcctgctcgc cgttagcgaa
     6121 gaaatcaacg ccgataaagg cggttaccag cgccgccagg cccacagagc caacgccgac
     6181 gatcgccgaa acgttttcag accagcgccc acgggagaac gccagcagga cgaagccaat
     6241 caatggcaaa ataatggtta aggcaagcat gttcatccgc gcatctcact tactgaatcg
     6301 atgttcaggt tctggcgacg acggtgaagt tgcagcagca gcgcaaggcc gatactcgct
     6361 tctgccgccg cgaggctgat ggcgagaatg tacatcacct gaccgtcggt ctgaccccag
     6421 tagcttccgg cgaccacaaa ggccagcgcg gaggcgttaa tcatgatttc cagaccaatc
     6481 agcataaaca gcagattgcg acggataacc agaccggtta agccaagaac gaataagatt
     6541 gccgcgagga tcagtccatg ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg
     6601 cgctgtcgtc tttacgattg ctcagcactt cacccgcacg ctcttcacga ccgacgtgga
     6661 aggccacaac cagacccgcg agcagcagca tagaagccag ttccaccgcc agtacgtaag
     6721 gcccgaacag cgtaataccc actgctttag cactgattgg cgtaccgtcg ataccctgat
     6781 cattaacacc aaggatggcg taaacaatca ccaccagcat gatggccgac aaaattgccg
     6841 gaccaatcca cacctgcggt ttcagccact ggcgttcctg ttcgatttct gaaccgccca
     6901 ggttgagcat catcaccacg aacacgaaca gcaccataat ggcacccgcg tagacgataa
     6961 tttccagcgc accggcgaag taagcgccca gtgagaagaa caccccggaa atcgccagca
     7021 gcgaaataat caggtacagc agtgcgtgta ccggattggt atgggtgatc actcgcaagg
     7081 tcgcaagtat ggctatcagg ccacagatat aaaaagcgaa ctccattgcc cctctcctta
     7141 cggtaacagg ctcttgacgt cgataggctt ggcttcgttc tctgcttcgc ccttatcttt
     7201 gccgtcgatt gccatacctg ccatccggta gaagttatat tccgggtatt tgcccggacc
     7261 ggagatcagc agatcctctt tttcgtaaac cagatcctgg cgcttgtatt cccccatttc
     7321 gaaatccggg gttaactgaa tcgccgtggt cggacaggct tcttcgcaca gaccacagaa
     7381 aatgcagcgt gagaagttga tgcggaaaaa ttccgggtac cagcgaccgt ctttggtttc
     7441 tgctttttgc agcgagatac agccgaccgg gcaggctacc gcgcagaggt tacaggctac
     7501 gcaacgctct tcgccgtccg ggtcgcgggt cagaacgata cgaccacgat aacggggcgg
     7561 cagatagacc ggctcttccg ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc
     7621 gatcatccag atactacgaa cctgggtgcc gaaacctact aacaattctt ttaaggtcat
     7681 ggtcttattg ccccttattg cgcctgccag agaatgacag ccgccgttac cagcaagttg
     7741 atcagcgtca gcggcaggca gattttccag ccgaaggaca ttacctggtc ataacgcgga
     7801 cgcggtaacg acgcacgaat caaaatgaac atcatcataa agaacgcggt tttcagcgcg
     7861 aaccagatga atggcggtaa caacgggcct tgccagccac cgaagaacag cgtcaccatc
     7921 aatgcagaga tggtcacaat cccgatgtac tcacccacga agaacagacc gaacttcata
     7981 ccggaatatt caatgtggta accatccgcc agttcctgct cggcttccgg ctggtcaaac
     8041 gggtgacggt gacataccgc cacgcccgcg atggcaaagg taataaaacc aaagaattgc
     8101 gggataacgt tccacacatg cgcctggctg ttgacgatgt cggtcatgtt gaatgaaccg
     8161 gcctgcgcca ccacgcccat cagggaaagc ccgaggaaca cttcgtagct cagggtctgc
     8221 gcagaagcac gcatcgcacc cagcaacgag tatttgttgt tacttgacca gcccgcaaac
     8281 agcaccgcgt aaaccgccag acctgccatc atcaggaaga acaaaatccc gatgttcagg
     8341 tcggcaacca cccaacccgg actgactggc acaatcgcaa aggccagcag cagcgaggta
     8401 aaggcaatca tcggtgccag ggtaaagatg acgcgatccg agaatttcgg gatccagtct
     8461 tctttaaaga acattttgat catgtccgca accagctgga gcgaaccgcc ccagccaaca
     8521 cggttaggtc cgtaacggtt ctggaacaga cccagcaggc gacgttcgcc aaagctcatg
     8581 aatgccccgc aggtgacaac caccagcagg atcaccaccg ctttgaggat ggtcagcagg
     8641 atctcaatca gttccggtga tatccaactc attgttgtgc ctccttgaga tcctcaagat
     8701 gcgcgccagc cagcaccgga gcaatgccgg acatacccat cggcaaaccc acctgccctg
     8761 ccgtcagccc ttcggcgatt tcaaccggca gcgtgaccgt gttgccatcg taactaaagg
     8821 agacgcgagc gcctgcgttc acgcccaact tcgcggcatc cgctgggttg agtttgatgt
     8881 acggctgcgg catacggctc tggaagaccg gagcacgctg tgacaattca tcgctgccaa
     8941 acaggtggta atacggcgcg atacgccatt tcccgtcctg cggctggaag cgtgccggta
     9001 cgctggtgaa gtaatccaga ccattttcgc tggtttcaaa cagacgcacg cccggatcgc
     9061 caaagcgcag tttgccgccc acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc
     9121 agcccggtgc ccaggcaaac ggcacttgcg aacggtgcgc ggtcggttgg ttgttacctt
     9181 ccatcgagaa ggtgaacatg gtgtcaatat cctgcggctg acgcggctca tgaacgctga
     9241 tattggcgcg catggcggta cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc
     9301 cacgaatacg gaatgtcgca tccggcgcag catctttgat acctgccagt tccgggattt
     9361 tcgccacaac agcgtcaatc acatggtcga gctgcgtcca gtccacttca cggctcagca
     9421 gggtgctgtg cagcgagtgt aaccagcgcc agctttccag catgacagtt ttgctgtcgt
     9481 aataagcagg atcgtaaacc tggaagaaac gttgggcgcg gccttcgttg ttgatcaccg
     9541 taccgtcgct ttcagcaaag ctggcagcag aaagtaccag atgggcgttt tccataatcg
     9601 ctgtgcgttg atgatcaacc accatcacca gcggcgcttt agccagcgca gcattcacgc
     9661 gggtagcgga agcgtgacga tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc
     9721 cggtttccag ttcggtcaac gcttcttcaa gcgaaccgcc acccataatg cccagcccca
     9781 tgctgttgac ggaacgggca atcatggtga taccaacgtc agcgccgcga cctttcaggg
     9841 ctttcgcgac gttagccgca gcctgaatca cctctatgct accggcgttc gtcccggaga
     9901 taatcaacgg tttcttcgca cctgccagtg cctgcacgat gacgtcgatt ttgctttgca
     9961 gctcaggttc gataccgtca accgctggcg cagagttatc cagcgcatgg gcgatggcaa
    10021 aacctaaacg cgcctgatct tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca
    10081 gacgggtgtc atcaacgttg gtaacaaaca gcggatgctt cgcacgttga ccgatgttga
    10141 ggattgccgc aatctgccag tcagccactt tctgtgctgc cgccatttcg cgcgctttac
    10201 ctttcacagc ctgacgcact gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc
    10261 ccagcaccag taccgcatcg taagattcga tttcgcgcag agccggagta taaatgccgc
    10321 cttcacgcag cactttcagc gccagttgca gacgttcctg ctcaccgtga gcgataccgg
    10381 tgtagaagtt ttcttcgccc accagttcac gcagcgcaaa gttgctttcc acgctggcac
    10441 gcggagaacc aatgccgatc actttcttcg actgacgcag aatatctgct gcaccctgca
    10501 ttgcctgttc ggcgttgagg gtaatgaaat catcgccacg acgctgtact ggctgacgcg
    10561 gacgatcttt caggttgacg taaccgtaac cgaaacgacc acggtcgcag aggaagtagt
    10621 ggtttaccgt accgttgtaa cggttttcga tacgacgcag ttcgccataa cgctcgccgg
    10681 gactgatgtt acagccgata gaacattgct ggcagatgct cggcgcaaac tgcatatccc
    10741 atttacggtt gtaacgctcg gagtgcgttt tgtcggtaaa tacgccggtc gggcaaattt
    10801 cgaccaggtt accggaaaat tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga
    10861 cgttgtcgtg cgcaccgtaa acgcccagat ctgtaccgtc agcgtaatct ttgtagtaac
    10921 gcacgcaacg gtagcaggcg atgcagcggt tcatttcgtg agagatgaat ggccccaaat
    10981 cctgattacg gtgggtacgt ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca
    11041 cggtcatatc ctgaagatgg cagttaccgc cttcttcaca taccggacag tcgtgcgggt
    11101 ggttggtcat taaccactcg accacacttt cacggaactg tttcgcctct tcgtcgtcaa
    11161 tggaaataaa ggtgccatcg gaagccggtg tcatacagga catcaccagg cgaccacgcg
    11221 tgtcttccgc gttttggtat tgcttcaccg cacactggcg gcaagcaccg acgcttccca
    11281 gcgccggatg ccagcaaaag taaggaatat caaggcccag agacagacaa gcttccagca
    11341 ggttgtccgc tccgttgacc tcgtattctt tgccgtctac atgaattgta gccattagca
    11401 tgcttccagt tttctcagtc agagactgag cgttaatcga aattcggtta ccagcgctct
    11461 ttcagcaggt tcggctgaat cccattaatc aaatgggtat tgctgaacgg ctgtttgatt
    11521 cccgcctcaa attcttcgcg gaaatatttg atggcgctct gtaacggctc cactgcacca
    11581 ggcgcgtggg cacagaaagt tttacccggg cctaagaatc gacacagttg ctcaagtgtt
    11641 tcgatatcgc ccggctgacc ttcaccacgc tccagcgcac gcagaatttt cacgctccac
    11701 ggcagaccgt cgcggcacgg cgtacaccag ccgcaggact cacgggcgaa aaactcttcc
    11761 agattacgca ccagcgacac catgttgatc tcatggtcaa ccgccatcgc cagcgccgta
    11821 cccagacggc ttcccgcttt accgatactt tcgaattcca tcggcaggtc aaggtgcgct
    11881 tcagtcagga aatcagtccc cgcgccgcct ggctgccagg ctttgaattt cagaccgtca
    11941 cgcataccac cggcgtaatc ttcaaggatt tcacgcgcgg tagtaccgaa cggcagttcc
    12001 cacaggcccg gatttttcac ccgaccggag aagcccatca gcttggtgcc agcatcttta
    12061 cttttcgaga tgttctgata ccactccacg ccgttagcga ggatcgccgg aacgttacac
    12121 agggtttcga cgttgttgac acaggtcggt ttaccccatg cgccggaggt cgccgggaag
    12181 ggtggcttcg agcgtgggtt agcacgacgt ccttccaggg agttgattaa cgctgtttct
    12241 tcgccgcaga tgtagcgccc tgccccggta tggacgaaca gttcgaagtc aaaacctgtt
    12301 cccataatat ttttgccaag cagacctgct tcggtggctt cggcaatggc acggcgtaga
    12361 ttcactgccg cttcgatata ttcgccacgc aggaagatgt agccacggta agctttcagc
    12421 gcaaacgcgg agatgagcat accttccacc agcaggtgcg gcagttgctc catcaacagg
    12481 cggtctttat aggtgcccgg ctccatttca tcggcgttac acagcaggta acggatgttc
    12541 atggattcgt ctttcggcat caggctccat ttcaggccgg tagaaaagcc cgcgccaccg
    12601 cgccctttca gaccagcgtc ttttacctga ttaacgattt cgtccggaga cagcccggtc
    12661 agcgccttac gcgcgccttc gtaaccgttt ttgctgcgat attcgtccag ccacactggc
    12721 tgtttgtcat cgcgcagacg ccaggtcagc ggatgcgttt cgggagtacg gataatgttt
    12781 ttcatttata ccgctccagc agttcaggga tcgcttccgg ggtcagatgc gcgtgagtat
    12841 cctcatcgat catcatgttt ggccctttat cacagttccc caggcagcaa gttggcagca
    12901 gcgtaaagcg accgtcaaat gtcgtttgcc ctggtttgat gtttagcttt ttctcgagcg
    12961 ccgcctgaat accctggtaa ccgttgatat gacagaccac gctgtcgcaa taacggatca
    13021 catggcggcc aaccggctgg cggaagatct ggctgtagaa cgttgccaca ccttcgacgt
    13081 cgcttgccgg aatacccagc acatcggcga tcgcgtggat cgcaccatcc ggcacccagc
    13141 cacgctgctt ctgaacgatt ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt
    13201 ggtgcatctc gtgctcaatc gcttcacgct ctgccgcact cagctcaaaa gcctcggttt
    13261 gtggttgttg attctcgtgc ataattagcg gtccacatct gacataacaa aatcgatact
    13321 gcccagataa acaatcaggt cagacaccag gctaccgcgg atcgccgccg gaatttgctg
    13381 caaatgcgca taactcggcg tgcggatacg agtgcgataa ctcatggtgc taccgtcgct
    13441 ggtcaggtag taactgttga tccccttggt cgcctcaatc atctggaaag attcattggc
    13501 aggcatcacc ggcccccacg acacttgcag gaagtgggtg atcagggttt cgatatgttg
    13561 cagcgtgcgc tctttcggcg gcggcgtggt cagcgggtga tccgctttga acgggccttc
    13621 cggcatgttg ttgaggcact gctcaagaat gcgcagactc tggcgcagct cttccacttt
    13681 cagcattacg cgggtgtagc agtcagaaac accaccacca accgggattt caaagtcgaa
    13741 gttttcatag ccagaataag gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg
    13801 caggcccgcg ccagtggtgc cccactccag cgcctctttc gcgccatagg cggcaacgcc
    13861 ctgggaacga cctttcagaa tggtattttg cagcgccgct ttctcgtaag acgccagacg
    13921 tttcggcatc cagtcgagga actcacgcag caggcgatcc cagccgcgcg gcaggtcgtg
    13981 cgctacgccg ccaatacgga accacgccgg gtgcatacgg aaacccgtga tcgcttccac
    14041 cagatcgtaa attttctgac gatcggtaaa ggcgaagaac acgggcgtca ttgcgccgac
    14101 gtcctgaata aaggtcgaga tgtacagcag gtggctgttg atacggaaca gttcggagag
    14161 cataacgcga atgacgttaa cgcgatccgg cacggtgatc ccggccagtt tctctaccgc
    14221 cagcacgtaa ggcatttcgt taacgcagcc gccgaggtat tcaatacggt cggtatacgg
    14281 aatgtagctg tgccaggact ggcgctcgcc cattttctcc gcaccacggt ggtggtagcc
    14341 gatgtctggt acgcagtcga caatctcttc gccgtcgagt tgtaaaacga tacggaaagc
    14401 cccgtgcgcc gacgggtggt tcggaccgag gttgaggaac atgaagtcct cgttttcggt
    14461 gccgcgcttc atcccccact cttccggttt gaaggtcagg gcttccatct ccagatcctg
    14521 tttggctttg gtcagctcaa acggcgagaa ttcggtagcg cgcgccgggt aatctttacg
    14581 cagcgggtga cctttccagg tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc
    14641 gaaagtaatg ccaaacagat cccaggtttc acgctcatac cagttagcgt tcgggaacag
    14701 tttggtgaag gtcggtacgt gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc
    14761 gcggttacga tcgatagaaa tcagatggta gaaaacggaa aaatccgcgg caggtaaccc
    14821 ttcgcggtgt gtgcgcagac gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg
    14881 tttcggcagt ttctttaaga aatcgccaac ttccagtaat tgttcacgct tgatccacac
    14941 aacgggaacc ccggtgcgag tcgcctgaac agtaaaggca tccggcccaa aacggttgcg
    15001 cagttcgcca atcaccggat catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc
    15061 ggttaagtcg gtcatattgt tcaccattgc aaatggtccg tggtgactgt cggcgcagta
    15121 agcttcgcga aatagattta ggaataagcg aagaaaaatc cctttgccga caggcgcaaa
    15181 ttaaatctcg tcaggtgtac gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc
    15241 gcgctctgat tgcatattgg cgcgataaac gccctgatcg ccaaccaccc aggagagcgg
    15301 acgacgttct ttgccgatag attcctgcaa cagcatcagt gcctgcatgt acgcttcagg
    15361 acgcggcggg cagcccggga tatacacatc aaccgggatg aatttatcga cgccctgcac
    15421 aacggaataa atatcgtaca taccaccaga gttggcacag gcacccattg agataaccca
    15481 ttttggttcc agcatctggt catacagacg ctgaataacc ggtgccattt tggtaaagca
    15541 ggttcctgca accaccatca ggtcagcctg acgcggcgaa gcacgcaata cttctgcgcc
    15601 aaaacgcgcc acgtcatgca ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga
    15661 aagaccgaag ttatacggcc aaattgagtt tttacgaccc cagttaacca tgtcattgag
    15721 cttgcccata aacacgtttt tgttaacttc ttgctccaga gggtcggtta cgatctcctg
    15781 cttttgcagg gggtaacggt cgttctcacc gttgggatct atgcgggtga gcgtataatc
    15841 catcttaatg cctcgcggtt agcgttgacg attagcgata ctgttcgttt ccgggttcat
    15901 acgctcgcgg cgtgaacgcg cgggcgtcca gtccagcgcg ccaatacgca ccagataaac
    15961 cagacctgcc agtaacacaa aaataaaaat tgcagcttcc acaaagccta cccagccgct
    16021 ttcgcggata gaggttgacc atgcgaacag atacagcgct tcaacgtcga agataacgaa
    16081 gaacatggcc accagataaa acttggcgga caggcgtaag cgggcggagc cgaccgagtc
    16141 gataccggat tcaaacggca cgtttttcga cctcgcgcgt gcgcgaccgc ctaaaaacca
    16201 accgcctacc agcatcaggc aacacaggcc aatggcaacg ataagaaaga tagcgaatgc
    16261 ccagtgatga gcgatgactt cagtggatgt tgacatactc attgcttact catcaaaagt
    16321 agcgccagat tcactgctct tcacggcaga tggacgccac atcgattcat ggggaggaat
    16381 aaaaaaaacc ttacaatcac tgtagaaatt cttttataca gctaattgat gtggtctttt
    16441 actcctttct ataacctttt gtcaacttta acaaaagttt cttcacatta gtttacataa
    16501 tatcaacacc attagcattt aatgcccttt caccccagat ccttgacgac tccaggataa
    16561 ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg tctattttga
    16621 caggaatttg tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct
    16681 tttgtcattc cataacaaaa acgcagcaac aaatttacgt attttttaac atcattgtag
    16741 caggtgattt ttttcaggcg attatttgtg cgttcgggac gtgaatctct ggtgattgaa
    16801 aaatgaacag ttttgtacgt tctgcactat gcgaggaagg atttttacta aaaaaagccg
    16861 ctggggttta aaacaccccc agcggctcgt tttttacact attgtctcag gaattatcta
    16921 tcgtccgtcg attactcgat atccctttca atcaacaatg aatcatcccc ttccggagca
    16981 gacattgggc tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctgg
    17041 gccagctcat tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc
    17101 ggtaagccat ctactccact cagcacacgc aggtccgggc tcatcatctc aaccggcctt
    17161 gccgtcacgc caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca
    17221 taagccagtc gccatggaat atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac
    17281 ggacttggat catccagcaa tacaagaggg atcggctcgc ctttttgcaa tacgtattcc
    17341 gctgcgcagt accagtgtgt tggcgaggtt cgcaggttca atgccttaaa cgctgaagga
    17401 cgatgggtgg ttaccatcaa atccacttcc tgggattcca gcatttctgc catataggca
    17461 ttacgtttaa cgcggacatc cagcgctaat ttcggataaa ccgaactcac gcgatttaac
    17521 aggaaaggta agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga
    17581 agattactga acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg
    17641 taaccaagaa gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac
    17701 agttctttcc caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta
    17761 cgacacacag cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt
    17821 ctcagcagat cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac
    17881 ttactggcgg ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt
    17941 tgtaatgtgc ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga
    18001 gttgcactaa aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat
    18061 atatcttcag ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc
    18121 gtgtttgtat aaatgtaaat gtgagtcctt gttccactct cgtgcagcat cgctggtcat
    18181 acgcgaacac gtacaaacag caatggtgag gcggcatcaa gagcagggat ccgtcattta
    18241 tgcgagcatt ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc
    18301 aaagttatgt tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt
    18361 ttgaatatta aataataatt aattagcatc atcctcattc attaattccg cttaacaata
    18421 gtttcacacc ttgcccccat tacgacacct cggaaatcat cacgctgtta aactctccgt
    18481 aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac
    18541 aattatctta cagataaaaa aaccagactt gcaattaaga atcagaacaa ttcactatat
    18601 aacattgcat gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca
    18661 caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
    18721 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
    18781 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
    18841 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
    18901 ctgaacatcg gcaacccagc cccgttcggt tttgacgcgc cagatgaaat cctcgttgac
    18961 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaaggtct ttactccgcg
    19021 cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat
    19081 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
    19141 agcggggacg aaatgttggt tcctgcacca gattacccac tatggaccgc ggcggtttcg
    19201 ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
    19261 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat aatcaaccca
    19321 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
    19381 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ttacgacgac
    19441 gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
    19501 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
    19561 aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
    19621 gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt
    19681 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc
    19741 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa
    19801 atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga tttcctgttg
    19861 caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
    19921 cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt
    19981 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
    20041 gcgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
    20101 gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg ctcatgcgca
    20161 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
    20221 tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
    20281 tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat
    20341 acttcaattc gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcaaaaac
    20401 tggtcgatat ggttccggaa gagctgcggg atatctttgc gccgttaatt gacgagcatg
    20461 catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga
    20521 aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
    20581 aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc
    20641 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag
    20701 tctgcgtcgc atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa
    20761 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa
    20821 cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagtgtgt taacaggtga
    20881 agaaaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg cgaatggata
    20941 aggcgacacc cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
    21001 cgcggtatta gagataaata gcccaataac cgccgacaag acaaacaaac aacccagcat
    21061 catatgtggc ccgtaaccac cgccaatgtc catcagccct ttcaccgcca gcgcgacacc
    21121 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
    21181 ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag
    21241 gcaggcgatg atagcggcaa caggattagg aatttcatct gtcagcatta acgccaccat
    21301 tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
    21361 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag
    21421 tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
    21481 accttccagc gccacgccat tgcgctttag ccccaccaca ttcagtccgt agcgggtacg
    21541 aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc
    21601 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
    21661 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
    21721 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
    21781 acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc
    21841 atccagccgt tgacctatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
    21901 ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg
    21961 cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
    22021 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
    22081 acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc
    22141 gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg
    22201 catggcaacg cttaacacca cgggaataaa gatagcgaca acgccggttg aactcataaa
    22261 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
    22321 cgccactttg accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc
    22381 gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac
    22441 ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
    22501 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
    22561 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg
    22621 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
    22681 acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg
    22741 acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc
    22801 gccaggccag aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca
    22861 agccccagca gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact
    22921 cgctcagcgg ttacaaacac ctctggtgcg ggaagcccag ctattttatg gcgcgctcgc
    22981 gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga
    23041 ttgagtaagg cgattgcccc cggaagcgcg gtaatacctt cggtttccgt ggcctcgatg
    23101 tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg
    23161 cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggtgctaac
    23221 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
    23281 gttccatcaa gatcaaacag aaaacctttg caccgcacac gggcctcctc aggcgttgat
    23341 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt
    23401 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccggc
    23461 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
    23521 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
    23581 gtcctgcaaa ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat
    23641 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa
    23701 tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
    23761 gagcatagtc atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc
    23821 cattgttgac tcctgtatca ctatactacg gtgaaaaaaa agaaggctga gtatgccttc
    23881 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
    23941 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
    24001 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg
    24061 cagggggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
    24121 ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc
    24181 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
    24241 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt
    24301 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
    24361 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
    24421 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
    24481 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
    24541 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
    24601 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
    24661 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
    24721 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
    24781 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
    24841 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
    24901 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
    24961 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
    25021 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
    25081 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
    25141 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
    25201 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
    25261 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
    25321 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
    25381 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
    25441 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
    25501 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
    25561 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
    25621 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
    25681 ttgtattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
    25741 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
    25801 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
    25861 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
    25921 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
    25981 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
    26041 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
    26101 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
    26161 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
    26221 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
    26281 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
    26341 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
    26401 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc
    26461 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
    26521 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
    26581 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
    26641 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
    26701 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
    26761 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
    26821 ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc
    26881 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
    26941 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag
    27001 cctgcagagc ttcaacctgg aagttccggt tgacgatcac gagcgtatcg agaaagttca
    27061 ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga
    27121 gcgcagccgt cgtctgtctc cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa
    27181 agcgggcaaa cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
    27241 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
    27301 ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggg attgaaatcg ttgatccaga
    27361 agtggttcgc gaaagctatg ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac
    27421 cgaaaccgtt gcccgcgaac agttggaaga caacgtggtg ctcggtacgc tgatgctgga
    27481 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
    27541 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
    27601 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
    27661 cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
    27721 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
    27781 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
    27841 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa
    27901 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
    27961 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
    28021 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
    28081 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
    28141 ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat
    28201 ctgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatt
    28261 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
    28321 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
    28381 tgttgctatt ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
    28441 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
    28501 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
    28561 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaaggattaa cctcaggatc
    28621 gaaatacggg acagctgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    28681 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    28741 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    28801 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    28861 gctgggctat gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt
    28921 tgccagttcg tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc
    28981 ggtgctttct ggctccgggt tgcgcatcgt ggtatgggtt atcgccactc tgattggcct
    29041 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    29101 tgagtccgac cgcttctttc gtgaaaagca ggctgatgtt gaacaacgtc cgtttacctt
    29161 tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt
    29221 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    29281 aattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
    29341 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    29401 agggattttg ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt taaataccat
    29461 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    29521 gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt
    29581 aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt
    29641 gctggcattc cagtttggtg atggcttcag tcacatcatt tacccaacct cagcttcgtt
    29701 aatggcaact ctcggtgtgt gcagggtgga cttccgtaac tggctgaagg tgggcgcgac
    29761 cctacttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    29821 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    29881 ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc
    29941 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact
    30001 gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca acgcgcccca
    30061 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    30121 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    30181 gaccacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    30241 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    30301 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
    30361 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    30421 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    30481 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
    30541 gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    30601 aaactccccc cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt
    30661 ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
    30721 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg
    30781 atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag
    30841 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
    30901 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac
    30961 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    31021 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    31081 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
    31141 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    31201 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca
    31261 aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    31321 cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    31381 cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    31441 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    31501 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    31561 cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    31621 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg
    31681 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    31741 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc
    31801 gcgctttcta tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct
    31861 ctatttcgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct
    31921 tgattatcgc ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt
    31981 gcgcatttcg cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg
    32041 cgaaccgtta agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg
    32101 actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca
    32161 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    32221 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact
    32281 gaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    32341 tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg caatgtatcc
    32401 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    32461 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    32521 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    32581 ataaagaacc ttcgtttacg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    32641 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg taccagtgaa
    32701 gatattaatg acgcgctgaa ctatcgcacc gtcaccaaaa acatcattca acatgtagag
    32761 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    32821 catcactggg tgacgtatgc tgaagtggag atcgataaac tacacgcgct gcgctacgcc
    32881 gattcggtat ccatgacctt gagctggcag cgttaaccgc catatcggga ggctgcatga
    32941 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg
    33001 agttgggcca tcaaatcacg gtagtgacgc gtaacccgca gaaagccagt tccgttctcg
    33061 gccctcgggt gacactatgg caagggctag ccgatcaaag caacctcaac ggcgttgatg
    33121 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac gagcaaaaag
    33181 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca
    33241 gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag
    33301 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg
    33361 cccgctggga agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    33421 gtaccggcgt agtgctggca ccggatggcg gtattctcgg taaaatgctg ccgccgtttc
    33481 gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg attcatatcg
    33541 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    33601 tggtttcgcc ctacccgata cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    33661 atcgcccggc tattttgcgc gtccccgcaa ccgccattcg gctgttaatg ggcgaatctt
    33721 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    33781 cgtttcgttg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    33841 tgcaaacatc cgccagttaa ctcccggtgt tacagggtta gtggctttgc gcgataagat
    33901 cgtctggtga aagtcgggtc accatcataa ctaactctcg gtctaaacct ctatccagca
    33961 tctcctgagc aatacgcagg gcttcttcgc gtttgccaag aattaatcca tcattccgcc
    34021 cttcttcacg taatctgtca gcaatggtca tcagtttctc cttttcctgt ggtgcgcgtt
    34081 ctgctatctc accaataaat gcacggaaac gctgggcatc tcccgtttgt aatacataat
    34141 taaacagggc ttttagctgt ctgtcattag tgttccctgt aactagcagc gaaacaattt
    34201 ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg
    34261 ccattttgcg atgttgcata atctcgtcat ccggcaccac ggtaatatcc accaacggaa
    34321 aagccgatga atatatttta cgggcaatcg caggctcggc aaattcatca agccagcaga
    34381 gtgaataagg ataaggactt ctgcaaccat gataaaacag cattgggatc accaatggaa
    34441 gctctttata gcctgcatcc agatggtttt gcatcgccgc gatggaatag cgcatcatgc
    34501 gaaaagccat taattcttcc ggcttacttt ggtgctctat cactacataa atataaccca
    34561 ctccctcctg cgttttcaca gaccataaga ggtcggaata atattgccgc aggtcgtcat
    34621 caataaaact gtttggttcc agtttaagcg tcgttaaatc acacagtttg cgcagcggcg
    34681 cgggaagatg aatatcaata aaatcccgcg cggtgtctgg atggcgtaaa aaagatttaa
    34741 ataccgcatc atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt cacccctcaa
    34801 atttaagatg acgcgacaat aaccacgatt aacacccaat gcagcaggca gtttttatct
    34861 ttggacagcg aattcagaag tatttttagc ggactgcaac ttttacattt cagcggaagc
    34921 tgactcgcaa attccccatg aaaatactcc ggcagcgtta gccaccggag aggaaattat
    34981 ttcagcgatc ccttaaggaa ctgttgcaga cgagggcttt gcgggttgcc aaataactgc
    35041 tccggcgcgc cctcttcttc tattttcccc tgatggagga aaatgacgtg agtagaaaca
    35101 tggcgagcaa agcccatttc gtgagtcact accaccatgg ttttcccctc ttctgccagt
    35161 tgctgcataa tacgtaacac ttcgcctacc agttcaggat cgagcgccga ggtaggttca
    35221 tcaaacagta aaacttccgg ttccatcgcc agcgcccgcg cgatagaaac acgctgttgc
    35281 tgaccgccgg aaagatgcac cggatatttc ccctgcgcac gttcgtctat cccgactttt
    35341 gccagatact tcaccgcccg ctcgcgcgct tcctgcttgc tcaggcccaa cacctgaatc
    35401 ggcgcttcca tgacgttttc cagcaccgtc atatggctcc agagattgaa gtgctggaat
    35461 accatcgtca ggcgtgtgcg cagtaagcgc agttgatttt tatcggcgac tttgagttga
    35521 ccgtctttgt cgcgcaccag attgatcgtc tggccattga ccacgatcga cccttcactc
    35581 ggtttttcga ggaagttaat gcagcgcaga aaggtacttt tccccgatcc cgacgatccg
    35641 atgatgctta ttacatctcc ggcattcgct tgcagtgaga cccctttcag cacttcatgt
    35701 tcgccgtagc gtttgtgcaa atcgataacg tttaatttat tctcggacat cgtattctca
    35761 gtgcgttgaa gaaggtttca catgctgcaa ccagcgtttt tccgctctgc gaaagaggct
    35821 gatcaggaca taagagatga ttaaatagag caccgcggca atgccgaagg cggtaaaagg
    35881 ttgatacgtg gcggcgttaa tatcgcgggc tattttcagc agatccggca cagtggccgt
    35941 aaacgccagc gcagtagagt gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc
    36001 aatacgcagc gcagaaggca aaataatgca acgatacatt ttaaaagtcg agaagccata
    36061 ggctctggcg gcttcaattt ccccatgcgg aaccgaacgg attgccccag caaaaatctc
    36121 ggtagtgtaa gcgcaggtgt taagcgtcag cgccagcacg gtacagttca ggccactgcg
    36181 gaagaaagcg ttaaggaatt cggttccctt aacaatctca agcgtgtaca tgccggaata
    36241 gaacaccagc aactgaacat acagcggcgt accgcgaaaa atataggtaa ataaccagat
    36301 tggaaactgg atgtatttat tactggagac acgaccaatc gccagaaaca gcgccaggac
    36361 tccgcctatc actaccgaca aaataagcag ccacagagtg atcgccacac cagtaaagcg
    36421 ataaccgtcg gtccacagca gcggtttcca gtattcatgt aagatttcga tcacaggtca
    36481 gccctcttca cacccacgga gtagcggcgc tcaaggaaca gcagcacacc attggaaaca
    36541 gtggtgaaaa ccaggtaaat cacgccacag acgatggcga aatagaacgg ttcccaggta
    36601 cttttgcctg ccagttgggt ggctttgacc acatcttcca ggccgagtaa cgaaaccagt
    36661 gcggtagatt tgaggatcac ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc
    36721 atcgccggaa acatgatccg ccgaaacact tgcccacgag taaaaccgaa cgccgtcgcc
    36781 gcctctatat gccctttcgg cactgccata aacgcgccac ggaacgtttc ggtaaaataa
    36841 gcaccgtaga taaaaccgag agtaataata ccagcgacca tcggatcgat atcaatctgc
    36901 ccgacgccca tcgcctccgt caccgtgttt agtgcaatct gcaaaccgta gaaaatcagc
    36961 agcatcaaca ccaagtccgg cacgcctcga atcagcgtgg tgtacccttc gaaaatcagc
    37021 cccgaaagcc gattttgcga gagcttaccg ccagcgccaa ttaaaccgat gattacagcg
    37081 agcactacag aactgatagc cagctccagc gtgacgagcg caccctgtaa aataacacct
    37141 gaaaacccat acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct
    37201 ccctccggtt acccgaaggg agcgggcttt ctttcaccga tggcgattag ccaccataaa
    37261 catcaaaatc gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg cgcatttcgg
    37321 caaaggcttt gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg
    37381 tccctacgcc aaacagtttt tcatctttaa cagacgggcc accgaatttg taatctttac
    37441 cgacaggttg tttgaggaaa ccttcgctgg cagcgacctc atcctggaac gcggcatcaa
    37501 tacgtccggc agtcaggtca gaataaatgt tgtcctgccc ctgatacgag acgatttcaa
    37561 tgccttttgg tgcccaatgt tcgttaccga acgtctcctg ggtggtgccc tgcaatacgc
    37621 ctacccgctt gcctttcagc gactcgactg tcggctgaat gtcggaattt ttcgccacca
    37681 ccagacgaga atcggcagcg tacagtttgt cggtgaaggc aatttcttgc tgacgttttt
    37741 ccgtaatgga aagcgatgac atgatggcgt caatcttctt cgcttttaag gacgggatta
    37801 acgcatccag cggattttcg acaaacgtac attgcgtatt gatgcgtttg cataattcct
    37861 ttgccagatc gatatcgaag ccaaccagtt cgccttgtga attttttgat tcaaatggcg
    37921 cataggtcgg gtcggtaccg atgcggatgt tttgcggaat cgcagcaaac gccgcagttg
    37981 cgctggagaa ggccagaacc agagagagcg atagcaccag ttttttcata atgtcctcaa
    38041 ctgacagtct ttttataggg aatatttaca ggtttgttac acttatcgtg ccataaaatt
    38101 gaccatcaag gcaaaatatc ccgccccgtt agacgtatta tttttcatta aaaatgctta
    38161 agtatgcatt tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca tgatgatcat
    38221 tccatcaggt acagcttccc agcgacgtat cagtcaccgt agacattaaa gtcgaaatac
    38281 tttttcgcca tcttgtcgta ggtgccgtcc tgacgcagct cgccaagcgc cttattgaag
    38341 gcagccgtca gttcagcatc atctttacgt agccctacac cggtgccatc accgaagtat
    38401 tttttgtctt ttactgatga gccagcaaag gcgaaatctt taccagcagg ttgcttgagg
    38461 aatccttcgc tggcagcaac ttcatcttgt aacgcagcat ccagacgtcc tgcagccaga
    38521 tcggaataga ccaaatcctg gttggcatag gccaccacat ccacgccttt gctacgccag
    38581 gtctcgttag cgtaagcttc ctgggttgat ccctgcagca caccaacatg tttacctttc
    38641 agtgaatcca gcgttggctg aatcggtgaa cctttggcag caatcaaacg agaatctgcg
    38701 gcgtacagct tgtcggagaa ggcaatctcc tgctgacgtt tatcggtaat ggaaagcgac
    38761 gaaataatgg cgtcgatttt tttcgctttc agtgagggga tcagcgcgtc aaagtcactg
    38821 gcaacccagg tacatttcac ctgcatccgt ttgcacatct cgttaccgag atcgatatca
    38881 aagccaacaa aatcaccttt agcatctttc gacgagaacg gcgcataggt ggtatcggtt
    38941 ccgatacgta ccgtctccgg tagcgccgca tagctggaag ccgctgcgga gagaccgact
    39001 aacaaagaca gagcgagaat cgacttcttc atacataacc ctcaagtgaa aatggctttt
    39061 ttatgttgtg tattgtgttg tgtgtttgca ggctctttca tgcaggtctt atgccatctt
    39121 gacgacaaca gtaacattca acgttaaata tgttaataag acgttgcatt attgtcctga
    39181 agttgaagat agcaggtata gcggttgaat cgcagcgttt cgattgaatg gcagaaacaa
    39241 aatgtcgagg atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac agcggagtta
    39301 tgcgccctgc cagcgggcaa agagatcttc aggaagggtt atcgcaaact ggtcaagaac
    39361 acgattaacc gtctgattta tcacatcatc aagggattgc gggcgatgat aaaacgccgg
    39421 aacgggaggc ataatcaccg caccgatttc tgctgcctga gtcattaaac gcagatggcc
    39481 taagtgcaat ggtgtttcac gcacgcagag caccaacggg cgacgctctt tcagcaccac
    39541 atctgccgca cgggtcagta aaccatcggt gtagctatgg acaatgccgg aaagggtttt
    39601 gattgaacag ggtaaaataa ccatccccag cgtctggaaa gaaccggaag agatgctggc
    39661 ggcaatatcg cgcgcatcgt gcgtgacatc agctaatgcc tgcacttcgc gcagggaaaa
    39721 acccgtttcg agggataagg tctggcgcgc cgcctggctc atcaccagat gcgtttcgat
    39781 atctgcgaca tcgcgcagaa cctgtaataa gcgcacgcca taaatcgcgc cgctggcacc
    39841 gctgatgcct acaatgagtc gtttcataaa tagttgccct gtcagacttg caggcagact
    39901 ttgcaggatt tcggcgggag ttgcaagtca gggtgccaga ccggcaccct cagcgaaggc
    39961 atcatccttc gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc actgctttgg
    40021 cgtcatcatt acgtaacgta tcgaggaaat cgaggtagcc ctgatcaaca tctttggtga
    40081 cgtagacgcc gttgaacacc gagcattcaa actgctggat atccggattt tcagcgcgaa
    40141 cggcgtcgat cagatcgttc agatcctgga aaatcaaccc gtcagcaccg atgatctggc
    40201 gaatttcatc aacttcgcga ccgtgagcga taagctccgt ggcgctcggc atatcaatac
    40261 cataaacgtt cgggaagcga atttccggtg ccgcagaagc gaggtacact ttcttcgctc
    40321 cggcttcgcg tgccatctcg ataatctgct cagaagtggt gccacggacg atggagtcgt
    40381 cgaccagcag gacgttttta tcgcggaact cggcgcggtt ggcattcagt ttacggcgca
    40441 cggacttacg acgcagctgc tggcccggca tgataaaggt gcggccaaca tagcggtttt
    40501 taacgaagcc ctggcggtac ggtttgccca gaatacgagc aatttccagc gcgatatcac
    40561 acgaggtttc tgggatcggg atcaccacgt cgatatccag atcttcccat tcgcgggcaa
    40621 ttttctcgcc cagtttcgtg cccatattca cacgcgcgct gtaaacggaa attttgtcga
    40681 tgaacgagtc cgggcgagca aagtatacat actcaaacag gcacggattg ctgaccggat
    40741 tgtcagcaca ttgacgggta aacaactgcc cttcttcagt aatgtaaatc gcttcgccag
    40801 gcgcgacgtc acgcaggaaa tcaaagccca gcgtatcgag cgctacgctt tcggaagcga
    40861 ccatatattc tgtacggttc tcgtcaatat cacgttttcc cagtaccagc ggacgaatcc
    40921 cgtttggatc gcggaaagca accataccgt ggccgataat catcgccaca caggcatatg
    40981 cgccgcggat taagcggttt gtggcagcaa tggcagcgaa aatattgtcg gcttccagcg
    41041 ggtagtggcg gaaattgtcc agctcgctgg cgaagatatt aagcagaatt tccgagtcgg
    41101 aagtggtgtt gatgtggcgg cgtttttctt caaacagttt tttacgcaac tcgtgagcgt
    41161 tggtcagatt gccgttgtgg gcaagcgtaa tgccatacgg ggagttaacg taaaacggct
    41221 gcgcttcaga ggcgctggag ctgccagccg tagggtaacg cacatgacca atgcccatat
    41281 tgccctgcaa acgctgcata tggcgagctt caaatacatc gctcaccagc ccgttcgctt
    41341 tacgcaaacg gaagcagtta ttggcatcta tggtgatgat gccggcggca tcctgaccgc
    41401 gatgctgaag caccgttaag gcatcataaa tcgactggtt aaccggcata acaccggcga
    41461 taccgacaat accgcacata cgtctttttc ctcgttaagc cacatctcag agcacttacg
    41521 ctctgggcaa gaaacttgac gagctttgca gataatcaaa aaaccatctg atgataaaac
    41581 tgaactgcgg gatcagctgt gatttgctcc agtcttcgct tttcgacacc ccggtaaagg
    41641 agtcgagaaa gaagagaatg gcggcaacaa tcaacacacc gcgcaacgca ccgaaacaga
    41701 cgcccagcac ccgatcggtg cctgacaacc ccgttttctc caccagctgg cctatcacga
    41761 agttcacgat agcaccaacg atcagggtag cgataaacag taccgcgatg gcaattccat
    41821 ttcgaaccag ttcgtcttca aagcccgtaa accagactga caggtaagtg tagtaatgac
    41881 tggcaacaaa gaaagcacaa ccccatgtca ccagcgataa cgcttcacga acaaagccgc
    41941 ggatcaggct aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa tcaatccaga
    42001 ccatatgtgt cccacgattt tacgccctgt catcctgttc ggggcgcatt ctaacagaaa
    42061 aagaaaacgt ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc gctgaaaaaa
    42121 tattcaacgc catcgacttt ttatgccttt gcggcatcgt gcaatgcgtg tcggatgcgg
    42181 cgtaaacgcc ttatccgacc tacggttcta acactgccca ggcctgataa gacgcgccag
    42241 cgtcgcatca ggcaagactg tattaattcg gcgtatagcc cattaccacg ccacttaagc
    42301 cagaaagttg cttcaactca cccagcgaac ctttcagctt atctttcgag gcatccggcc
    42361 caaccagaat acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat
    42421 aaccggcacc gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgcac
    42481 ccagttgcac aacataggct ttacccgttg gcgctgcttt ttcttccacg accggcttcg
    42541 gttctggctt cggcgcaggt ggtgcttcaa ccttcggttt aggcggctcc accggtttcg
    42601 gcttcggtgg ggcgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag
    42661 tggtcggatc gagcgacggt gctgcggcat cacctgcccg cacctcttcc gctgcgcctt
    42721 ccggcggctg cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcgcgat
    42781 cgcccgcttt cggcaccagc gggatagccg cgaactcatc ctgataatgt tttttctgcc
    42841 cgtccagcag ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta
    42901 accgattctg aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt
    42961 gcgacagtgt ggaaagaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt
    43021 gcggcatccc atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc
    43081 agttgttctg ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc
    43141 acgcttttca accaggccag agttccggca atatctttat catgtagcat accgataacc
    43201 gccagcacgc gcccgttttt cggtagcgct ttcatacgcc cggtgagata ttccgccgca
    43261 tgtggattat gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt
    43321 cccggcaaaa ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ttccagcccg
    43381 ctggcacgca gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc
    43441 agattttcca gcgtgccgtg agcatcgcta aacgcccagt catgatcggt gacggaatag
    43501 ttccactcaa cgccccgacg ttgtaacagt gcaccttttt cctgtgccac atcagcaatg
    43561 gtagaaggca tttccggctc accgacaatt gccggttttt cgctgcggaa gatgcctgct
    43621 ttctcgcgac caatactttc gcgatctgga cccagccagt cggtatgatc cagcgcaata
    43681 ctggttacta ccgcgacatc ggcatcgaca atattggttg cgtccagacg accgcccagc
    43741 cctacttcca gaatcaccac gtcaagttgt gcctgcttga acagccacaa cgccgacagc
    43801 gtaccgtact cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa
    43861 gaggcggtgt gggccgattc cggcaattcc tggccctgca cacgtacgcg ctcggtataa
    43921 cgcaccagat gcggtgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac
    43981 tccagcgtac ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc
    44041 gctggtttca gaacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagatcg
    44101 atagttttac tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct
    44161 tgaggagtgc gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag
    44221 ggcagagcca gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc
    44281 gggggtacca ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac
    44341 ttcgccagaa tgctcgccag tttcaggcgc atttccggac gacggacgat catgtcgatc
    44401 gcgcctttct cgatcaggaa ttcactgcgc tggaatccag gcggcagttt ttcgcgaacg
    44461 gtctgttcga taacacgcgg accggcaaag ccgattaacg ctttcggttc agcgatgttg
    44521 agatcgccca gcatggcgaa acttgcagaa acaccgccca tcgtcgggtc ggtcagcacg
    44581 gagatgtacg gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc
    44641 tgcatcagcg acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc
    44701 agtgggcagt tatcttccag cgcctgctca acggcacgca cgaaacgtgc acccacaaca
    44761 gaccccattg aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca
    44821 tacagagtgc ctttcatcac caccagcgca tctttttcgc cagtttcttt ctgcgcagat
    44881 gccagacggt ctttatactt cttggagtca cgaaacttca gcacatcttt cggctcaagc
    44941 tcgctaccca gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct
    45001 gtcatacgca tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg
    45061 cggtataaaa cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc
    45121 ttgcgggtgg gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct
    45181 gtctgaacct ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc
    45241 caacagacca tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt
    45301 ggtcgaaccg cggagttact ttttattttg cggcgcgtgc cgcagcgcgt ttgtgacgaa
    45361 taaattcgaa aaccccaggc aatattgaaa caaaaataat gccaacaatc atcagcttaa
    45421 ggttgctctg aatgaagggg agcgtgccga agaaataacc cgcgtaggta aaaatcagta
    45481 cccacaacag tgcgccgatc acgttataag cggcgaaatg acggtacgac atgtggccca
    45541 ttcccgcaac aaacggggcg aacgttctga cgatcggtac aaaacgggcg agaataatcg
    45601 ttttgccgcc atgtctctcg taaaactgat gagtcttatc aagataactg cgacgaaaga
    45661 ttttcgaatc cgggttactg aacagtttgt caccaaataa ccgtccaatg gtatagttca
    45721 ctgcatcacc gacaatagcg gcaatcaaca taagcaccac catcatatgg acattgagat
    45781 cgttagtttc cagcgacgcc aatgcaccag caacaaacag cagcgaatca ccaggcagga
    45841 atggcgttac caccagcccc gtttcgcaga acagaatcaa aaacagaatg gcataaaccc
    45901 agacgccgta ctccgcgacc agctctgcca gatgcacgtc aatgtgcagg ataaaatcga
    45961 tgaggaaata aatcaggtcc atatttgcct atgccttgta ctcgtcatct ttcaggctgt
    46021 aactacgttg gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat
    46081 agcgggccca ttggcggttt tggaagatca taccggtcag ggtaatccac cgcgaccaga
    46141 tacagccctt ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac
    46201 tctgctatcc agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata
    46261 ttcctgacca tatgatgtac aaaggcattc gctttgatat ctaccaccac ataaggaccg
    46321 tgacgcgtga cattaatatg cataacgttg cgccacgggg ttcgggactg gcactgcacc
    46381 gcacggaacg aggtgaaatc attctcgccc agcaagcatt gcgcagcccg atgcatccgt
    46441 tcagcgtcca gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc
    46501 cgatgattgt agatgatgta gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa
    46561 tcatcaggta cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg
    46621 cccaaggtcc acgccgcgtc tttgcgctgc gcggtcgttt cgaaatgcac tacctgcccg
    46681 gtaccgtgta caccagcgtc agtacgcccg gcgcagaaga cggtaatggg ttcgttcgcc
    46741 acctgggaga gcgccttttc cagcttctcc tgcacactgc ggacttcatt ctgccgttgc
    46801 cagccgtaat acttactgcc gtcgtactca atgcccagcg caattttata aactggcggt
    46861 tgttgctggt cggacattag tacagatact cctgcaccag tttctcggcg atttttactg
    46921 ccatcagcgc gccgccaaag cgaacgttat cggccaccga ccagaactgg acttgctccg
    46981 gcataccgta gtcattacgc acgcagccaa cagaaagatg cggcgtaccc gaagcatccc
    47041 ctacctgagt cgggaattcg ttctcttcag agagcacaat atcttcgccc tgagcaaacg
    47101 catcacgcgc ttcttctgcc gccagcggac gcagtgcttc aaagttgacc atctgggcat
    47161 gaccgtagaa taccggtgcc tggacgacgc ttgccgaaat catcagccct tcgtcctgca
    47221 ggattttgcg tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg
    47281 gcaacaacgg cagcatattg aacgccagtt gacgaccgaa gaaatcttct tcgtcaatcg
    47341 gaatgccgtt gagcaatttc gcactctgcc ccgctaacgc atcgaccgct tttttgccct
    47401 gggcggaggc tgaaatcagg ctggtaacac tgatacgcga caaaccgccc tgatcgatta
    47461 acggtttcag tgccgccagc agctggctgg tcagactgtc tggtacggcg atgacattcc
    47521 ggttgcggta atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg
    47581 gttcgagagc aaacagtccg ctgctgtcga tcaccaggca acctgagttg gtcgcttctt
    47641 caacccaggc agcggtagct tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc
    47701 agtcgaattc agcggcatcc tgcacggtga ttgtcttacc accaaagcgc agttgttcgc
    47761 ctgcgctttc gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca
    47821 gcgtttcaag cagggcttcg cccacagcgc cagttgcgcc caggacggca atgttccagc
    47881 cttcagacat ggtggtttac tccagaaata gcaaagctcc ctgccaatgt tacagcagag
    47941 agcatgaaga agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag
    48001 caatgatgcc gcactggcgt catcacaaat tacatacaga gatgaccatt cacggcgctc
    48061 aagatagttt ttgcgcagtt tatcgaactc acccggtatc ccggcgactt tacgcagctg
    48121 tgcgtcatcg cggcgcacat catacaccaa atgcaccagc cttttcagcg tcggttgatc
    48181 gagcgggcca tgcagcgtaa tgcgaccaaa ctctggcgcg ggcagtaatg tatccagcgc
    48241 aacgtgctgt tcatgcccaa taaacttgct ataggcttca aacacttgcg tagtaccgcg
    48301 tgctttacct tccagggtat aacctgcgat atgcggcgtg ccgatatcca cttttgtcag
    48361 caactccacg ttaagttccg gttcgccttc ccagacatcc agcactacgc ttaacttctg
    48421 gccttcattc aggcaggtca gcaacgcagt attatcgacg actgcgccac ggcaggcgtt
    48481 aatcagaatc gctcccggct tcaggctacg gatcagtttt tcatccgcca gatgcagcgt
    48541 tttgtacggc ccatctttga agagtggcgt atggaaagtc agaatatccg catgctgaac
    48601 taattcatcc agcgagcgga aatcaccctc atccccacgg tcagcgcgag gcggatcgca
    48661 aagcaaggtt ttaatcccta acgcttccag tcgcgcttgc aatcgacgtc caacgttacc
    48721 aacgcccacg atccccaccg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat
    48781 cagtagtgag gagaaaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga
    48841 aaaaccaatt cccgcctgct tcaaccatgc ttcatcgaca tggtcggtcc ccgcagtggc
    48901 agtgccaaca aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga
    48961 acgcaccatc agcgcatccg cgtctgccag ttgagcgacg gggattggac gcccgggaac
    49021 cgcggtcacc tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac
    49081 aaggattttt aacccttttt cagtctctga caatctaagg ctcctttatc aacaaacgcc
    49141 catttgcaaa ctgctgttta atctgaaaaa acattaataa tcatacagat aacaacataa
    49201 ccatttagac gggcaatact gaaaaattgt tattcagtat cgccctttaa gccgtttcag
    49261 gctatataaa attaatcccc cgtggacact gcgtggacac ttacgccacc tttcagcgga
    49321 ttaagggcca ccgcatcctg caggtaatcc ggtgcaaaat gcgcatatgc cattgtttgc
    49381 tgaatggttg cgtgaccaag aatcttctga agcgcaataa tgtttcctcc gttcatcaca
    49441 aaatggctgg cgaacgtatg ccgcagcaca tgtgcagcct ggccttttgg taaatcgggc
    49501 ttaactcttt tcagcgccag gcagaattcc cggtacttca cctcaaacaa gccgcctgtt
    49561 tctctggttt tgatcgcctc acaaactgcc tgcgaaattg gcactgttct cttccggcca
    49621 tttttggttt caagaaacgt tacacggtta tgaactatct gttcaccacg aagcttacaa
    49681 gcttcactcc agcgcgcccc tgtgcttaaa cacaaaagcg caacacgcca gtagtcgccc
    49741 tccagtgtat cgagcaatag cgccacttcc ttctgtgaca ggaacgccat ttctcgtgga
    49801 gatacataaa gaatagaaat tccccttact ggatgttctg catcccaaag acctattttt
    49861 ttcagcacgg taaacattcc ggataaccga ttcatgtacc tgttagcaga tgacggtttc
    49921 aatccatcag ctatcttttg agaacgccac gcgataattt tcagcttatc aagatccaca
    49981 gcctgcatat cagcgccaag ctcattgatt atgttgcgca gttgttttcg gtcttcttcc
    50041 gccttacgcc tgtgctggcc gtgatacatc caccacaact caagcaaatc atttagcgtt
    50101 cgacgatcac ggtagccctg tatatattcc cgcttttcag cgttcgccat gatgtagcgt
    50161 tcagtggcca ccgctaccga ttttttgtca aataccttac gcacgcgctt tcccttgcgt
    50221 ccgttcggcc tgatgtccag caaataacga ccatcttcga gcttcttaat cgacattacg
    50281 aagccctcca atgaaccgct ctacaatttc tccagcctct ttccagcaat aatcagacca
    50341 gacaaaaagc aggtctaacc agttttctgg cctcagcggg ataatttttg gattgtttcg
    50401 gttgaaacgc gatctccctc cgaattgcag gaaccatcaa gagagagagc cggagaaatt
    50461 tgcccgacct ccggcatcgt ttcatctgtt gataaccaat aagcatactt cttaaattta
    50521 gggtgtttcg taaccttaag taaggcaccc tctgttactt gcttccccct cacctcatag
    50581 ttagttaccg tcccataagg cagcccaaca caatccgcaa actcctggcg ggtcatacct
    50641 tctgcttcac gaatcaggcg aaatttttca cccatgcttg acaaagatgc catatcgggc
    50701 atatcctcca tcacaacatg ccacatcggg cacaaacata aaaacactca aataagccga
    50761 tataagccat tttgagccat tcgaacgaat tagggagatt accacaatga gcgaatcaga
    50821 gcttgggggg ttcattcagg tagcaccata tccacttgaa gcggtgccat atcaactatt
    50881 cgccaagatg atcggccgca aggaatccac agtcagaacc atgattgacg cagcaaagct
    50941 accaacaatt gactttgtga aaccaggttc agtaaagacg cgtgcatcag aaaactgggt
    51001 atatctgcca gcatttaacg aaggcatgcg caaagcgttt tttgagcaac cgaaagaacg
    51061 ccgcgacgca tggttgttgt ggctgggact ttagtcataa atgaccagcc atatcatcag
    51121 cgccattctg acccttgttt ttattgagat aggcgtaata gagatctatt tgtttcgcaa
    51181 gttaacggga cataaagaac gctttattga actcagcatt gaatatatcg ccgcttatac
    51241 caaaggactt ttcccggcag ctattggggc gatgttgata gcgtttgtta tctggtttat
    51301 cgggtgagaa acctgacaac ccacaactac tgcaatgagg gtaattatgt gtggcatgga
    51361 caaattggac cttatgttaa ttgtaatact atcaattaac ttcggttacc tcttaagcgg
    51421 cgcaattctc atgtgcggag gcaaacgaaa atgaaccagc agtccgcaaa acgtgaaaac
    51481 aatgcgatgc gatttaatcg caaatatttt gagttcggcc tctatgccgg aataattaaa
    51541 tctgtacaga gtctttaaca tgaaacagca acgtaattca cgctttcgca atggtgcaga
    51601 acgccacgct aaccgttttg ctaccagtgc atcacgcagc aacatccgct acagcctgag
    51661 tgatacacac gcaacgccgg atggctaccc agtaaaacaa atcggcgagc atgcctggct
    51721 gattgagaaa gctggaatcg tggtccacaa atgcccgcgc aatccgttta ccggaaaccg
    51781 catttttgct ctgagcagcg gcgacaatca gttcgggcag gatttcacat tatacgaagc
    51841 gcttcgcacg gttgatcgtc tgctgcgtgg acagagtttt attaaacagg ctgatttata
    51901 acaggtgctt tatgactaaa gaccatactc aaggtgtatt tatccgcttt attgattttc
    51961 gcggtgaact gttattacgc gcatccgcta ttgatggagt ggctccggcg gggaaaaacg
    52021 gagccgccga agccacttac gtttatctga acggcacgcg actgatcgtg gaacttccgt
    52081 accagaccgt acgcgaaatc attagcgaag ctgaaaaggc tcgtcaggtt aatggcgata
    52141 aaccctatat cgaaattatc tgtatggatt cagaaactga aattcagaaa gcagattaaa
    52201 gggcgttgcg atgggcaaag aatataaaac tctcattaac aaagcacttg agcgctttta
    52261 ttttcgctta agtgcatcag gcgctcatgc tgaacgtgcg gcccgtgact cattgaccag
    52321 agcaatccga agtctgtatg acgtggcttt ttacgctgat gatctggatg cacttaacga
    52381 actttccgag ctgatctgtg ccgcagaatg cggggaacat attgaaccgt atgagctggg
    52441 gaatatcgca tgagtatatt tatctcatgg cttgttctga ttatttcggt ggcctgcgct
    52501 attgggatta tgcgaattat tcattcaata aaaaagattg aacgcttttt cactggcgaa
    52561 taacagagca aataaaacca cagattaaat aagaaaatgt aaaaacaatc cgcattcgcg
    52621 gaggtattcg cacacgccaa ggaggcgtaa tggcaattaa gcattttcct gtcgttcgtt
    52681 ttacctccag agggcgtgaa tacgaagtcg acgaacgcct gattaccaca atcgacaaac
    52741 accgttcaga aaaggatgca catcacatct atctcactga cggcacttac ttctgcgcca
    52801 cgaatgtggt gcgggtgaac cttatccgac aggtacagga gtcacgcaga tgaccattct
    52861 ggactatatc gccgccaatc cggggtgtag cggtggagaa atcgccgcag cactgaatac
    52921 cccaaccaca gccattaatg cggagttacg ccgactctgg cgcagcggtt cagtcataag
    52981 aaaagagcgc aaaacaggcg gtcgcttttc ttaccagata aacccgatgc cgttcgggtg
    53041 cggcaatcca cttaccaaca cgtttaacca gctactgaag gaagccagag catgagcgcc
    53101 atcaaccacc aggaattacg cgaactggcg actgacctgc aacgaatggc aacgcctcaa
    53161 aaattactgg cgtttcgcgc aatgctctcg ccgtctgctg tgctggcgct gctggatgag
    53221 ctggagcacg ccagaaccac acctctcgcc attcgcctga cgctccgtca tgaaatcgag
    53281 gatttctgcg cgacgttgga ggcgccaggc gaaccggaaa cgccgaaagc aatacagcaa
    53341 gagctgctgc aacgcattga caaggttttt gatttttttc tgaaccacta agaaaccaga
    53401 acatgcacac acaaaaaaac cgcttgccat gccgcaatca gtcaggttac atttccgctg
    53461 cacctcacaa aacgggtgcc gggtttcgca gcctgctgac tacacaagcg cacaaccgcg
    53521 ccagcggttt ttttgtgcgt actgtattgc cacgtttttt tcgcgtcaga attatggcgg
    53581 ggcgtacggg gccgacttcg gtcgggccgg gttcttgtgt agccggtact gcgaacctcg
    53641 tacgtctcgc cacccacagt ttcgcagctc tggatggtga gttttcacaa cttactacac
    53701 aaggggccac accatggcaa accgcaaacc acaccgcgct atcgcggagc gtcgtcacat
    53761 ccagactgaa atcaaccgca gactttcccg cgcatcacgc gtcgcgcaaa tcatgcacat
    53821 caatatgttg catgagcgca gccacgcact atcaaacatt tattccgcct ctgttttcag
    53881 ctatctggcg gatgatctgc acgagcttca acagctcatc cagcagcaaa acaaactcca
    53941 ttaattcctg ttccgggcct ttcctgcacc ttgcggcggg aggccttcgc acatctgtaa
    54001 caagaggatt gccgcaatga ttctcgccaa cgactttctt gaatacctgc tcaacacaga
    54061 gcgtgatctt gccgctcgcg tgcgtgatcg ttatgacatg tacctgaaat ccctgcctgt
    54121 accgcagctc gctgacggaa agattgttat tgatggtcgc tacatgattg acagccacga
    54181 gggaaattac aggctttacc gcattgaagg tggcaccccg tccgttattg gcatttacca
    54241 gcgcccatcc tctgcaatcg tcgatgtgat tgccgacagc atccgcatca cacatcgcca
    54301 tgccgacaca gaagacaccg tgctggaaat tcagcggctg gctacagtct gccgcgacac
    54361 cctgaatggc atgacgaagt aaatcactat gacggcagag tacatcaggg actggcaaca
    54421 accgcgccac gcagtggggc gtgaaggaac ggggatcccc gctcctgaat ccgcgctttc
    54481 ctcctggctg gatgcctacc gggtagagaa cgagcgccgc caggaaatgg ctgatgcggc
    54541 gttctccgcc acgccgctgg gcaacctgat taataaaagc ctggacgcac aggaaaaaca
    54601 ggacaaaacc atcacactgg caggagacgc cagaaaacag gcacgcggcg cggtggatga
    54661 agccatggcc tcgctgcgcc tgctgccgtc ctatctgcgc gatccgctta ttcgccacct
    54721 ctccttcctg cgcaaaaaac aggaagccga tcgccggaaa ggcaaaaaga gctggcaggc
    54781 tgaacgctac gcgtgcggaa ccctgcgcaa aatattcgaa cgtctggacc gcaccgacca
    54841 ccgctggctg acaccgggtt atcgctccct tgccggacgc gaacgcctgg acgatttgct
    54901 ttacctgccg cagctcaaca aacaccagat acagacgctg gccaccatga cggcggcgat
    54961 gttcagcagc accttcgaaa aactctgcga tggctttggc gcgactgatg gcgagctgac
    55021 catggatgta acgctgaagg cgtatcagat gctggcccgc atggcgttac acctgcacgc
    55081 catgcctccg cattatgacg cactgacaac agacaaggac cggaggaacg caccggatac
    55141 ggaactgctg ccgggcgcaa tccttcgcct gacctgtgcg gaatggtgga aacgcaaact
    55201 gtggctgtta cgttgcgagt ggagagaaga acaactccgc gccgcctgtc tggtttccag
    55261 aaaaacatca ccctatctga gccaggacgc gttaagcgag tttcgcgcac agcgcgagaa
    55321 aacacgcgat ttcctgaaaa gtttcatgct ggaaaatgaa gacgggttca cgattgatct
    55381 cgagacggtg tattacgcgg gagtaagtaa cccggttcac cgtaaggcag aaatgatggc
    55441 caccatgaag ggactggaac ttctggccga agcccgtggc gacagagcgg tgtttctgac
    55501 tgtcacctgc ccgtcaaaat accacgccac aacagagaac ggtcatccga atcccaaatg
    55561 gaacggggcc accatgcgcg actccagcga ttacctggtt aacacgtttt ttgcggcggt
    55621 ccgcaaaaaa ctgaaccgcg acggtcttcg ctggtatggc atccgcacgg tggagcctca
    55681 ccatgacggc actgtgcact ggcatatgat ggtctttgca catccggacg agattgaaac
    55741 catcgtgtcc cacgtctgcg atattgccat tcaggaagac cgccacgagc tgggcgatga
    55801 cataactccg cgttttaaag cggagtacgt cgacggctca aaaggcacgc caaccagcta
    55861 catcgccacc tacatcggaa agaacctgga cagccgcgcc gtggatggta tcgacccgaa
    55921 aacgggcaag ccacgcgttg accacgaaac cggaaaatca atggccgaga gcgtggagcg
    55981 cgccatcggc tgggcgcgcc ttcaccgggt ccgccagttc cagttctttg gcatcccctc
    56041 ccgtcaggtg tggcgtgaac tgcgccgcct tgccagccag atggcacgca acccggaagg
    56101 cccgcaacga ctgaaggatg acgcaatgga tgcagtgctc gctgccgctg atgccgggtg
    56161 ttttgcctcc tacattgaaa aacagggcgg cgtacttgtt ccacgcaaag actacctgat
    56221 tcgcaccgcc tacgacctcg cagatgagct gaacgattac ggcgaacaga gcgtacagat
    56281 ttacgggatc tggtcaccac tcatcgggga gtcttcccgt gtgtgcacac atccggataa
    56341 ctggaagctg gtaagacgta aaccggaagc ggaagacagc gcccgcgaaa atggttttga
    56401 ccttcagggc ggccctgccg ccccttggac tcgtggcaat aactgtcccc gtgtacagga
    56461 aacagacaac aacgggacag aacagccgga agaacggcca gcaccgtggc cgcagcttcc
    56521 tgacggcgtt gacgtgaatg aatggatgcg ctcactgaaa cggcacgaac gccgggagct
    56581 gatgcgtgcg cttcgtgaca aacaggcaaa aaacagcagt gatgaaatgc agagctggac
    56641 acagagccgc aaacagcagc ggcctttgcc tgataaccac gaattactcg ctaaagaatg
    56701 gcgggagtct gctgaatctc tcggcctgca tatcggtgaa caacagatgc agcacctgtt
    56761 acggggcggc agtctgtacg ttgacggcag catcattgca ccgcagagat ttgaaattgt
    56821 acgcaaacca gatacccgac cggacagccg aatcacgcag ctctggcagc gcctgagccg
    56881 taatcacggc gtaaacagca cggaaatccg ccataacccg gtttccagct atctgaaaca
    56941 gctcggggca tcagacccag aagccgctgc acgcctggca tccacaattc agcaggacca
    57001 gaacaccatg aaaactcccg ttaccgtgct ttctgacatg ctgcgcgcca tccgtgacgc
    57061 agaacacgca cagagaatca atgaaaccac tgaacgcgca cgtcacaagg cgagtctgtt
    57121 gcaggacaaa ggcaatagcg agaagaaaaa ataaccaaga aagaaatcat caattgtatg
    57181 aaggtagagt ttagacatgg taaggacgtt ccatgagaca cactgtaatt tcatatagta
    57241 ttcctcttcc ctgtttgaca aaaaagtgca atactataaa tagtccagtt aaatgctttt
    57301 tcaattcact taaaatcaag aggttacagt taagatgggc gacttaggtg gtattcagtc
    57361 agatttagcc caactagtta gactggtaat agctgaacag tatgatgacg ttcggttata
    57421 tgtcgctcgc ttggtgcgta aatatcggga aactatgcct gctctatccg agcagcttga
    57481 tctttatcta cgaaataaac cgcaaaagcc tgcacaaagc ctgcgtaagg ttaccgcacc
    57541 aaaacaagcg ctgcctcaaa ccatgcctgt ggacgaggag tcaaggctta ccttgcttaa
    57601 agcaccaaat gaaaagattg tgagcaaacc tctactgtcc agtcagatag aagactcact
    57661 ggatcaaatt attcttgagc ggaagcacat cgatcgcctc aaggctctcg gcttacaacc
    57721 aacacgctct gccatttttg ttggccctcc gggtgtaggt aaaacactta cggcaagctg
    57781 gttagctcag aaattagggg ttccttttta cgttcttgat ttaacggcag taatgagcag
    57841 ctatctgggc aaaagcggaa ataaccttcg ggcagctcta gattttgcga agaaagggcc
    57901 gtgcgttctt ctgttagatg aaattgattc aatcgccaaa aagagaagtg atgattctga
    57961 tgttggagaa ttaaaaagac ttgttaccgt gatactccag gaggttgacg aatggccgtc
    58021 ttctagtttg ctacttgctg caactaactt cgctgagttg attgatccag cactatggag
    58081 acgttttgat ttagtgctca attttgagaa gccagatagc gaaagtatta aagaagcaat
    58141 taaacgcttt tcaggtcccg actacgcgat ttttgcacgt tggatagatc tattggctat
    58201 tatgttcaaa aatgaatctt ttagtaatat tgagcgttct atcaacaaat tccgacgttc
    58261 ggtcgcttta ggtatttcct cagatgaaga gttgattggg tcgttcatta aagatggttt
    58321 atcagaatta gacaggaatg aacgtaaaga aattgcagtt actctaaaaa gaaattctaa
    58381 attatcccag catgccatat cagacttaac cggagtcagt agggatacaa ttagaaaata
    58441 cagtcaaaaa atgtaataca gcatagataa ggagtatgta tggcgaaaac taattttctg
    58501 attggacgcg gcgaattatt aaccagcgat attcctggtc ctaaaagagt gcccggaaaa
    58561 gctgaggttt attctcttta cgaatcgaaa caacgtttat tgccgcaaat tgcatctact
    58621 atagaaaatt ttgatgattt acccagtcag gcatgtcctt atgattatgc tgtggctaag
    58681 ttgacgttga atccgagcta tattgcacgc tctttttttc ctgcaaaaat gcttaagtct
    58741 accgggttaa cgtctattgg cagcaggacg gtaaaaatta cgcctcaaag ctggaagcga
    58801 aaagttactg tgtcagaagt aactacgaca caaatgtttg ttgctggtaa aagagaatcc
    58861 ttcaaagagt tacctcattg ggtgtcagga ctcaaagagt tttctgacga agcgattgat
    58921 tttgcacgca tagaacggtt cgatgcctat ttacccgaag agcgtattat taccattggt
    58981 gagcaggatt cttcgtattt tgaggttggt gttcatttac tgccagataa tgaacaagac
    59041 ttggttcagc aacaatttgt tcagtttgca tcaaatttga acatcaaagt acatacttca
    59101 ttagctttta ccgctggtac attatggttc gtgccaatcc aggccgatag aattactatt
    59161 agtaagcttg cgaaatttac atttgtacga gtaataagac cggttccaag attgcgtggg
    59221 attagaccaa tacaaaggaa ctacggtcct ttagctcagt gtaaattgcc tgctgagggc
    59281 cccgtatctt cagaaataaa agttgcgatt cttgatggtg gtttaccatc agagcacgct
    59341 atatcgccat ggttaaaatc ctatagagtt ttggacgatc atgcagagga tgatccagac
    59401 ggtttagagc atggtttagc tgtgtcttca gcctttttat ttggaccaat aagtaataaa
    59461 tcggaagcga aaagaccata ctcttatatt gataacttaa gagttttgga caacaagacc
    59521 tgccaagaag atccgctgga actatatcga actttgggct ttattgaaga ggtattgttg
    59581 tctcgccagt atcagtttat caatctaagt ttaggtccag acctaccaat tgaagatacg
    59641 gaagttcatg catggacttc cgttattgat gatttgttaa gtgatggtga aacattaatg
    59701 actgttgctg ttggcaacaa tggagaaatg gatcgcaagt ctggtaatgc aagaattcaa
    59761 gtaccagccg attgcgttaa tgctcttgct gttggtgctt gtgatacagt taatgatacg
    59821 gaatggaaac gagcaccata tagcgccatt gggccaggca gaagtcctgg cgtgatgaaa
    59881 ccggatttag ttgcgtttgg tggtgacaca gacgagtact ttcatgtttt aggtaaaggt
    59941 aagaagcctg taatcatacc ccagctagga acaagctttg cctctcctta cgccttacga
    60001 gcagctgttg gtgttcgggc aattttaggg aacgatttga gcctactagc aatcaaagct
    60061 ttgttaattc atgcgtcaaa acagcttgat catccttgta cagaagttgg ctggggaaaa
    60121 ctgcctgaag acttgaacga cattatagtc agccctgatg gtgttgcccg tatcgtctac
    60181 caaggtgaat tgaaaccagg aaaatactta cgcgcttctc ttccaatacc tgaaggtggc
    60241 ttgaaaggta gaataaactt agcggcaact ttctgttatg caacaaccac tgatccacaa
    60301 gacccagctt cctatacaaa agctggtttg gaagtgacat tccggccaaa tgattcaaaa
    60361 atcaaagacg gaaaacaaaa tgctgaaacc aagggattct ttgaactggc caaatattca
    60421 actgaaagtg agcgacgttc tgacatgggc aaatgggaga ccgtcttaca taattcgaag
    60481 aacatgctag gttcaactct aaaaaatcct gtatttgaca ttcattataa cgctcgtgag
    60541 gccggtgcaa gcatagccag ccataaggca gaaaaaatca agtatgctct tataattacc
    60601 attaaggcat caaagcacca agacctgtat aacgaaattt tacgtactta taatcaggtg
    60661 ctcgtgccaa ttcaaccgca aacatcaatt cctattcgtt cgtaatcctt caagacataa
    60721 tgcccctcaa tttgaggggc atttctttgc acaaaagtgc acaaatttgc acaatttttt
    60781 tgaacaactt tttacccttc cggcccgcat ggcggctgga tccgtcaagg atccgtgcgt
    60841 gcacaaaaaa acgcgttttt tctgcgcgca ggtgacgggg gaacagcccg cgtttcaggg
    60901 ggtaaacagc attccctgaa cgatgtcgca gcgacacaac agaatggctg tatcgctcac
    60961 gctgagcgtg aaaaagacgt aaggggttct gatttgatgg gatgaaaggt aaggccgtca
    61021 aaatcgcact gaggcggcga gaacatgcag tcggcgcggt gggattgtgt aagagtctgg
    61081 ccgtcgatga tagcgataag tcggaagatg tcgtgaaatt atctgattga tacgggagct
    61141 ggagagccgg ggcgtaaatt ttttatgccc cggcgaagca gcagacaagc gaagcgcgtc
    61201 agtgatgcgg caccttgccg accacacttc ataagtgcaa aatacgagca aagaaatcaa
    61261 tggaggctgt tttatggtca ttaattacaa gcagttacga gaaaaacgag agcaggtaaa
    61321 ggaaagtttt cgccgcaatg aagatctgac cccgcttgta caccttgccc agggaattgt
    61381 tgatgcttat gaaatctctc tggagctgcc atcacagaca tggacggata gcgacggtaa
    61441 tcgccagcat tacgtttcat gcggactgga aacaactgaa gggtttcgca gaatgccttt
    61501 atctcagatt cctgccgcta cccccaaagc aaggggcggc aatgatgagc gaaaactgat
    61561 tttcagcatt gagacagtgg ttgacgacac acctggcgaa gtcgcgttcg tgcatactcc
    61621 tctttcgatc gcaatgtata acgatgaagt acaggttcgc gttaataata atatcgtgcc
    61681 acttaaagaa ggtaattcac catacaccac cgtttgtgaa gccattcaat attacgttct
    61741 ctctgaaatt gataatctca agcctgacgg cacccagaaa atggttcaac tctggtaaaa
    61801 aggacagccc catcacgggg ctgttttttc atcaagaaga gcataagaat taaaacggat
    61861 cacctcttcg ccaagccagt cattgatgtg cttcatggcc tccatgacag gcatcagctc
    61921 gttaattgcg taaacccgcg cagccttctc cacatcacca aacgcacttt tttcgcccgg
    61981 catcgccccc atcagttgcg gcggaacgcg gtgcgcagcc agcacatcat cacgggatgc
    62041 cgccttaaca ttcatgaact catcctttgc ggtgatctgc tggaacggca aaatttgcac
    62101 cccctctttg cccccgttgg gcgcatgaat gagcacgttt ttaaacgcac caccaccacg
    62161 tgccccctgt agcgtttctt tcagggagtc catgctttcg cggttgacct gcgctgcacc
    62221 gatgtagatg atgcacccgg cgtgggatcc gttgtcgtaa tacagttttc tgaacatgtc
    62281 cgccgaatga gacaggctgg ccgagagtaa tgcaccaaga tattccggca tgccgtagat
    62341 ttcctggtta atgtcaggat tcatcaggtg gcacactttg ccagggcgaa actgaaacgc
    62401 gtccttgcca tcctgcacat accaccatga ttcaagatcg cttccgcgtc gcatgtattt
    62461 cgccagtgcg tgccgtaatt taagtggttc gccgagcata ttgctccgaa gctcaaggaa
    62521 tgcgttaccg aacacaaacc agtccagcgc cagcgccgag aaatcctgcc gggaaagcag
    62581 cggatgcgga atatagcagc cgagcaatac attacgctta aagtaaagcg cagactgatg
    62641 ccaggacgtt tgccgggcag ctcttgccag accgtaccag tccaccgggg tttcatacca
    62701 ccgcccgtta tcagcacagt acatattgtc cagcaggtca tgcccggtca ggcggtaagg
    62761 accatcaaat gtgaatgcac tgagcgacga ttctttcctg agcgcatcag cgagatcaat
    62821 gcgtgaactc atgcgcactt ttttattttt tctgctcatc agaactccat aaccgtgaaa
    62881 cgctcgtttt ctccttcgcc gccaattggt tcgttaatga cagcaagcat ggttgcccac
    62941 gcaaggtcgc cgtggctgat ccccctcgcg cggtccgttt cgtaagtgat aaagccgccc
    63001 ggtgttttca ccttacgcac ggcgttaaag gccgcgacca gctcgcgttc ggcgcgatcg
    63061 tattcccacc gcccggcacg cattatttgc agcattttca gtaccagcga ccgttttgat
    63121 gacagcgtga aggtgtacgg aatagcagca gggaaaaacc gtttcactat ctgataaaca
    63181 gcctccccgt tcccgcccgt cacatcaatg ccgatgtgtt ccacgttgta gcgacacgtg
    63241 aactcttcaa tgactctggc ctgttcttca aactccagcc cctgaacgcg tcgcgtctcc
    63301 accgttcgaa aacggccacc aggaacagcc ggaggaacca ccacggacac cgcgccactg
    63361 tcgccgtttc cactgctgcc gtttgcgtca tacccaatcc ataccggacg attccccatc
    63421 gggcggggag caaaaggttt ccagtctttc cagtcgtcgt atccatcaac gccgcagcca
    63481 atcaggatat tcaggttaaa tgccgattcc ccttcgcgga caaactcaca catatagaga
    63541 ttgcggaact cgtcttcggt gttttcatca cgaatttcat caatatcggt gtgtttccag
    63601 ccgtgattaa ccacatcttc cagcgtgaca atttgccgcc acgtccggtc ggggcagata
    63661 agcccgttat gcagcgtttt ccagtccaca gaaaaatgct ggcgtttatg cgcggccttt
    63721 ttctcgttcc agcggtcgcc gttccagtag acgtatgcct cgtgcgtttc ggttgatggc
    63781 gtggagaagt aggtgcgtcg cagcccgctg agggttgcca tagcgccagc caccttgcgc
    63841 agttcagcaa agcgactgac ccagaaaaat tcatcaaaat aaaaattgcc tgtataggac
    63901 tgtgccgtcg cagcagaagt accgagaaaa tgcagctctg cgccgttgga gaggatgatt
    63961 ttatcgcccc ctttcagctc cacatcaact tcagatgcag ccttctgaat aatgctttta
    64021 aactggaacg cctgacgacg cgacgcagac aaaaaaatct ggttacgctg gtaaggttgc
    64081 gccacatcgt cacgcagcgc catcagcagt gcttcctgtg caaaatacca ggtcgcccca
    64141 atctgtcggg atttcaggat catcctgtta cgtatcccgg cttccctgca aagggtcagg
    64201 gagtcaaacc agccccgctg atgccgctcc agcctgctga tgattttttc ccgcagtgcg
    64261 gcaatctgtt ccggcgtgaa atgatttttg agttttttcg cccggccttt ctttcctgcg
    64321 gccatcacat ccggctggcc atcatgcagc tttttaagct gccgggtcag caggtctatt
    64381 tccttaaagt caccgcctgt tttattctgt ttttcagtaa gctggatgag gcgcgcatca
    64441 atggattgcg tgacacgctg cacgggtggc gtttcatccc actggtcgcg ttttttccac
    64501 gcataaatcg tgttcgggtt tattcccatc agacgtgata tttctgcggg cggataaccc
    64561 tgccagtaaa gttgtcgcgc acgctggcgc acaaaagcgt cctgaatcat tgctccccct
    64621 gagtaattac aggaagatta cccgcgcgcg aaaccgttct ccttaacccc ctgttctgac
    64681 tgttttctta caacaaaagc cctttgtatc agcctgttac gctttgccat catgactgaa
    64741 gaaccagtca gaggggcaaa aactatggct aatgaaaaaa agacatcccg caaaaagttt
    64801 cgcgtggctg tctccggtgt aacggcagac gggcgcgaaa tcaacggcga catgctgaaa
    64861 gctgccgcca ccagttataa cccgtccgtt tatggtgcac gtgtgaatat tgagcacatc
    64921 ctgtcaccac tccccggtag cgagttttcc gctatgggcg atgttgtggg gttgagcacc
    64981 gaagacataa ccaatggccc gctggcaggt cgcacggcac tgtatgccga aattgagccg
    65041 accgctcgca tgatgtccct gcttaacgat ggtaaaaaaa tttactccag tattgagctg
    65101 gaaccacagt caaccatcac gggaggccct tacctgcgcg ggctggcaat gaccgacacc
    65161 cccgccagcc tgggcacgga acgtctggcc tttgcggcac aacaacgtat gcaactgatg
    65221 acattcaact gtcagcaggg agacgtggcg atgtttaccg ccgctatgga gtcagaactt
    65281 atcgaactca ccgaacaacg tcaggaagaa ggcacccagt ggtttaaccg cgttatgggg
    65341 attattggcc gtggccgcaa agcggatgac gccagtttct cccgtattca ggaagcggtg
    65401 gaaggtgtcg caacgtcaca ggccgacatt atcgaccgtt ttaatgcgct ggaaacccgc
    65461 catcagcagg acagccagaa aatcacttta ctgaccacag agctggcagc actgaaggaa
    65521 aaactgcgca cgcaggacgg cgatccgcag aaccggttca ccgcaacagg cgcagcctcc
    65581 gaccagctgg ctgacttctg ataagacaaa ggagcaaatt ttttatgaat ctggtgatgt
    65641 cagatattac ccgcaacaag ctgggttgct atatggcgca gcaggcgtcg cttaacaata
    65701 tcccggtttc cgcactggta tcgcgattta ccgtggaacc ctcggtacag cagcgttttg
    65761 aaaacgcctc aaaggaaagt accgaattta caaaaagaat taacgtgatc ggcgtgaccg
    65821 accagaaagg cgaaaaaatc ctcctggata ccaccgggcc gattgcgcgc acgaatacca
    65881 gttatgacgg cacaaaacgc cgtaacccga ataacgtggt tgatctgaaa aaccgcaaat
    65941 accagtgcga acaggtgaac tacgacacgt ttatttcata tccgcagctt gatgcctggg
    66001 cggcacaccc tgattttcag tcccgcatca gcgcacagat tgcccggcag gtggcacttg
    66061 accgcatcat gatcggtttc aacggcacgt ctcacgcgga tgagtccaac ttcagcacca
    66121 acaagctgct tcaggacgtt aacgtggggt ggctggagca catcagaacc aatgccagcg
    66181 agcgcgtaat gaatgacgtg acgctaactt cccgcaacat ggacaacacc gtggcgcacg
    66241 cgggtaaata cgcgaaccct gatgcactgg tacaggacgc gcgctcatcc ctgctggatg
    66301 aatggcacaa ggaagctgac gacctcgtgg tgattatggg gcgcaacctg tttaactcgc
    66361 tgcgtctgcc cgtgctgaac agcatcagcg gccagaatcc caatgcggaa ttacttgccg
    66421 ggcagctcat cctgtcatcg cgcaccattg gcgggctggg cgtgttcctt gcgccgttct
    66481 tcccggatgc aacgatgctg atcacctcat tcaacaacct gtcgatttac tggcagaaag
    66541 gttcaatgcg tcgtctgatg aaagacgaac cggaatacaa ccgcatcgcc acctaccagt
    66601 ccatcaatga cgcttatgtc gttgaagact atggcaagtg cgcgatggtc actggcctga
    66661 agttcgccga cagctaatca actcacggcg ggcatcatgc ccgcctgtaa cggagagaaa
    66721 aaatgattac tcctgcacag caacactggc agaacgtgat ggcacagcgc gcaggccggg
    66781 cgaatgaagg cgtggaccac gccgcgcgta ccgcgcatga agaggtgctg tatcgtctgc
    66841 gtctggcaca ggcccggctt aagggcgtac aggccagaag cgcgaaagcc gccatcaaaa
    66901 aagagttgtt gccggacttt tccggctgga ttgagggaac gctggaggct gacggcgggc
    66961 agcaggatga agtgattgcc acgctgatgg tgtgggcgat tgactgcggc gatcttccgc
    67021 tggcgctgcg tattggtgca tatgtggtcc gtcacaacct catcatgccg gataactttg
    67081 gacgtactgc cgccacggta ctgaccgaag aaatctgcaa cccggtactg acgcaggccg
    67141 ggacggatgc cgacgcggat ttatccgcct ttatcgaacc actggacaca ctttgggaaa
    67201 ttgtcgccaa ccaggacatg ccggacgaag tgcgcgccaa attatgcaag gcgtgtgcct
    67261 ttgcccgtcg tggcctgacc gatgcagaca gcatggcctc atcactgaag ctgctgcgcg
    67321 aagcgatgca cctgaacccg aacgcaggtg tgaaacgcga gattgcaacc ctttcccgcg
    67381 ccctgaaaaa agccgattcc gcagacgcat cagaagacgc cagcgcacag caggcgcagg
    67441 acgaaagcag caaaagtaaa aagacaacgc ggaagcctgc aacacgaaaa accaccgcga
    67501 cgcagaaggc gaagcgcggt taacgactga ccccgtcagc gggcggcgtg cgcggtgttc
    67561 cggtttgact ccgtgaccgt ttacaccgcg cacccaccgc ccgatttttt caggagtgaa
    67621 ccccatgagt atggttgcca gaaccaaccc cggccccgca gaggacgaca tcaccgatac
    67681 cgatgatggt gatacccgta tttcagcggg cgcattctgg ccggatattg tgctgcgtga
    67741 gctgcgtctg gcggtacgac tgccgggccg tgtgaccacc tcccgcctgc tgcataccgc
    67801 caccggggcc gtgacacacg ttacccgcga gctggaagcg tggcagcagg aacagcaggc
    67861 ggctggccat cagacgctgg ccgatgttcc ggcacccgta attaacggag aaagcgtcaa
    67921 tctctggcac tggcgcaatg ctgtttatac cgccacacgc gccctgatac tggagcgtta
    67981 ccgcgatgcg gacacaacgg acaagggcga ccgccgggcg gacgcactgg atatacagac
    68041 atcggatttg tggcgcgatg tgagctgggc catctctgac attctgtgcc gcccgcgaat
    68101 ctttgcggag ttgtgctgat gaaagtgaag gcactggaag gcgacaccgt ggattcgctc
    68161 tgtttccggt actacggcac gacgcagggc gtcaccgaaa aggtgctgga tgccaacccc
    68221 ggactctgtc agcaggtatt tctggacgcc gggcaggaag tggagatgcc ggagccggag
    68281 aagaagaaac gagaaatgat tcagttgtgg ggggagtagc agtgagcacc attcaaacag
    68341 ggatcacaga gcaggttatt gcatggctct ttgaccacct gccaacggtg tatgcagtag
    68401 gcgcggcggt cagcatttcc gcgctgatga gtctttatga cggacggaca ctggttcaga
    68461 ccgtaacggg atcgctggcg tgcggcgttc ttgccatggc cgtggccggg tcgttgcgct
    68521 tcttcgggtt tcctgaagat gccgtgacgt ttatcggcgc atcaatcggt tttatgggtg
    68581 cagagaaagc acgcgacaag gttattgcgg cctttaatcg cagggtgaag gagaaggacg
    68641 aatgagcaac acatttaaat tcagcagccg gagcgaaaag aatttgcagg gcgtaaatcc
    68701 tgatctggtg aaagtgaccc gacgggcact ggaaatctcg gaagtggatt ttggtatcac
    68761 cgaagggttg cgcagccgtt accgccagaa gcaacttgtg gccacaggta agagccagac
    68821 catgaacagc cgccacctta cgggacatgc cgtggatgtt gtggcttata tcggcagcca
    68881 ggtgtcatgg gaatggccgc tgtacgaaaa aatcgcagcc gcattcagac aggccagccg
    68941 ggaactgaat attccggtgg aatggggcgg cgactggaag accctgaaag acggaccgca
    69001 ttttcagtta ccacacggag cctatccggc atgaagctct ggccaacgct tggcgtcgct
    69061 ttccttctga ttgccggatg gggaacatcc atgcgtctgt catggtcgct gggccgggag
    69121 aacgccagaa acgaagcgca ggccagcacc ctgaaacgta ccgccgacac cctgaatatc
    69181 atcagcgccg gggtacagga tatgcagcag gtgctggcgc aactccgcgt ggaaaatcag
    69241 caacgcaatc aggacggaga ggcaagacgt gaacagctac gcaacgatat tgcaaaagat
    69301 gaatgcgccc acgctttgcc tgacgctcgt tttactgaca ggctgcgcag gcacgcagaa
    69361 cgcgctacgg ccagcgccgt cagtccggct tataccgcag acgttgacca taccggtaac
    69421 gcctcccccc ttccctgaca ctcccacatg gggaaatctc ggtatatggg gcgaccgcct
    69481 tctggatgca ctggaaacct gtaacgcgga taaacgggcc attgaattac tggaacagcg
    69541 caggctgcaa cgactgaaca acgaggacaa caaccatgct gaaaactgat tccctgcgtg
    69601 aagccatgac ccgttcatgc cgatggtgtc aggccaaccc ggaaaaattc accattttcg
    69661 tggagagcgg caacattgaa acgaccggag aaacgccctc gtttgtttac cgctatcaga
    69721 tggtgatgtt tgtcatggat tacgccgggg agctggacga cctcacgttg ccgctactgg
    69781 cgtggttatc cgaaaatcag ccacagttgt tgctcacccc tgagcgtaat caggacatca
    69841 aattctccgc cgttatcaat gacgatgaca gcgccgacct cctgtttacg ctccccctgc
    69901 gggaacgcgt tcgcatcacg cgcaacagtc agggcacacc gcaggcagaa cacctgccgg
    69961 agccaaaacc ccgtctgccc tcttccgaag gcgactggtc gcatgtattc caggatgtga
    70021 cgtggggtga aagcgatgga taaggcattc acccgcgtgg atgaaacctt tgaggccatc
    70081 cgcgacagcc tgaatcagca ggccatcaat aacatcgcca gaaagctggc acaggattta
    70141 cgccgcgccc agcaggcgcg tatccggtca cagaaagcgc cggacgggac cgcgtggaca
    70201 ccacgcagac gccgcgtaac ccggatacag gaacgcattc gctttatctg gaataacgaa
    70261 gcacgcacgc tgaaaaactg gcatcacgac acggggaaat acgggcgaac cattaccggg
    70321 tgggatgagg ataaaaacaa tatccgcacg ttttaccggg atgacatcga ccgttttctg
    70381 gaaatacgca cccggcgcat caaccaggac agcacaaagc gcgtccccat gttcgtaaaa
    70441 ctgcgcaccg cccgctacct gaaagcccgt gcagatgctt ccggtgtgac ggtgggttac
    70501 agcggcgtgg ccgcacgtat tgcccgcgtt catcagttcg gtgagcgcga tcaggttgcg
    70561 ccgggcattt tcaccgatta cccggtacgt gagctgctgg gtatcagcca ggcagatgag
    70621 cgcctgattt ataacacggt gctgggccgg tttgcggagg ctgtacggtg agcgcagaac
    70681 tcatgcgact gctgagcaat atcatccgca ccgggatcat ctctgaagtt gatgagaagt
    70741 cctggcgcgt gcgcgttcgc agcggcgaac tggaaacagg atggttgcgc tggaacacca
    70801 cgcgcgcggg agccttcaat gtgtggctgc cgccatcacc aggcgaacag gtggtaattg
    70861 cctgcattgg cggcaacccg gaaaccgcca tgataattgg cagcctgtgg agtgatgcca
    70921 gtccggcacc cggcaaaagc ctgaaagaaa tcgtggtcag cgcgccggat ggcgcggtgt
    70981 tcagctacga cgcggacgca ggcgcactga gcgccagcgg catgaaaaca gccaccctgc
    71041 aggcatccgt cagcgtgaca ctggacacgc ccgtcgtgga atgcacaaac cttctgagaa
    71101 cagcgacgct tgacgtcaca aaaggaggaa agatgagcgg caatatcacg cacagcggcg
    71161 gcgatttcac ctcaaacggc atcacagtgc atacgcataa acacggtggc gttaaaggtg
    71221 gcagcgattc gacaggaggc ccgcagtgac aacccgctac acaggaatga acccggacgg
    71281 aacgggaaac ctgaatgata tggaacacct gaaacagtca gccagggaca tcctgaccac
    71341 cccgctggcc agccgggtta tgcgacggga atatggcagc cttgtgcctg atttgattga
    71401 cgaacccatg aataacacca cgcgtctgca atgcatgagt gctgccgtga ttgcgctgac
    71461 acgatgggaa ccccgcattg ccctggatgc catcgacgtt gtctggaaag cgggaggccg
    71521 cgccggggtg acgctgtcgg gcactgtcat gcagaccatg cagaatgttg agttaaccat
    71581 cacgctgagg gagtaaatca tgcccgccgt tgacctttcc cagttaccgg aacccgccat
    71641 catcgcggag cctgactttg aagcaattct ggctgacaca aaggccatga tgattgcgtc
    71701 ctatcctgcc gaacagcgtg aagccgtctc cgccgcgctg gagctggaat cggaacccct
    71761 gaacgttatc gctcaaacca tgtcttttcg tgaaatgctg ttacgccagc gggtcaatga
    71821 gggtgcacgc gcctgcatgc taagccacag cgccgggaca gacctggaca acctcgcggg
    71881 caatatgaac acaaagcgcc tggttatcac tccggcaacg gataccaccg acgcggtgat
    71941 ggagagcgac acctcgctga gactgcgggc gcaacgggcg tatgacggtc tgagtgttgc
    72001 tggcccgtca ggcgcatacg agtattttgc acgcagcgcc agcggtctgg tacgcgacgc
    72061 gcgagccatc agcccgtctc cggccaacgt gacggtttcc atcctgtcca ctgaaggcga
    72121 cggcacagca acggaggcgt tgcttaatac cgttcgcgcc gttctgaatg cagaggatac
    72181 ccgcccggtg gccgaccgcc tgacggtaca gagcgccaga atcgtgacat ggcggctgaa
    72241 tgcaaaactg tacttttacc ccggcccgga atccgaacct attctggctg cggcggaatc
    72301 gtcattcagg aagtggctgg ctgagcaggg gcttatcggt caggacgtgg cgttgtccgc
    72361 cattgctgcc gcacttcatg tgcacggcgt gcaacgcgtg gagataaccg aacccacaca
    72421 gaatatggcc atcagcgaca tacaggcggc gcgctgtgag tcattcacca tcagcgaagg
    72481 tgggcgcaat gagtaattcg ttgttaccac catcagccag caatttcatg cgttgtgccg
    72541 aagccgtcgg aacacgcatt acagacattc cggtagacct caacacgttg tggtcgccgg
    72601 acacctgccc ggtgcatctg ctgccttatc tcgcctgggc attttccgtt gaccgctggg
    72661 atcgcaactg gccggaagag acaaagcgac aggtgattcg tgatgcatgg ctgatacacc
    72721 gacacaaagg gaccatcagc gcactgcgcc gggccattga gccgctggga tacctcattc
    72781 gtgtgtctga gtggtgggag ttcggcggag aaccgggaac atttaccgtt gaagtcggca
    72841 cgctggacag tggtgtgacc gaggaaatgt atctggaaat ggagcggttg attgctgatg
    72901 cccgcccggt cagccgccac atgacagggc tgaatatcat tcaggagatt cagggagata
    72961 ttttcgcggc ggcagcaact tacgacggcg aagtcattac catttatccg gacgattaag
    73021 catgagtacc acaacacgta aatttaaaac cgttatcacc gatacgggtg ccaaaaaatt
    73081 agctcaggca gccgcgccag atggtaagcc tgtccgcctg actcatatgg ccgtgggcga
    73141 cggtggcggt acattgccca caccagacag taagcagccc cgtctggtgc atgaggtgtg
    73201 gcgacatact gttaatcgcg tcatcctgga cgcaacacat cagaaccgca ttattgcaga
    73261 gctggttatt cctccagaaa cgggtggatt ctggatccgg gaaattggtg tatttgatga
    73321 gcacggcgat ttaatcgcgg tgggcaatac tgccgaaagt tacaaaccaa ccgttgccga
    73381 agggtccgga cgtgcacaaa catttcgcac cattctgacc gtatccagca ctgccaccgt
    73441 ggcgcttacc gtggataaca ccatggtgat ggccacagtg gattacgtgg atgacaaact
    73501 gaaagagcat gaacagtcac gacgtcaccc ggacgcctca ctgaccgcaa gaggctttgt
    73561 tcaactcagt agcgacacta acagcgtgtc tgaaacacag gctgcaacac caaaggcggt
    73621 aaaggcagca tatgaccttg ctaacgggaa atacactgca caggacgcca ccacagcgcg
    73681 aaaaggtctt gtccagctca gtagcgtcac caacagcacg tctgaagcac tggccgcaac
    73741 accgaaagcg gtaaagacag catatgacct tgctaacggg aaatataccg ctcaggacgc
    73801 cacgacagca caaaaaggga ttgtccagct aagcagcgcg actaacagca catctgaaac
    73861 gcttgccgcg acaccgaaag cagtgaaagc agctaatgac aatgcgaatg gtcgggtacc
    73921 ttctgcccgt aaggtgaatg gtaaggcgct ttcagcggat attacactga cgccgaaaga
    73981 tattggtacg cttaactcaa caactatgtc attcagcggt ggtgctggtt ggttcaaatt
    74041 agcaacggta accatgcc
//
LOCUS       DAZFFI010000011        62315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFFI010000011 DAZFFI010000000
VERSION     DAZFFI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.011"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1729
                     /gene="adeD"
                     /locus_tag="ACHJ12_000968"
     CDS             <1..1729
                     /gene="adeD"
                     /locus_tag="ACHJ12_000968"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4988796.1"
                     /translation="RAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGR
                     YIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTV
                     ICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLA
                     WRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGI
                     ENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPW
                     EIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLS
                     DARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPG
                     KRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPSCGLLGGFGLN
                     EGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGL
                     MSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKF
                     AFTTLEVTE"
     gene            complement(1775..3166)
                     /gene="uhpT"
                     /locus_tag="ACHJ12_000969"
     CDS             complement(1775..3166)
                     /gene="uhpT"
                     /locus_tag="ACHJ12_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4988797.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3304..4623)
                     /gene="uhpC"
                     /locus_tag="ACHJ12_000970"
     CDS             complement(3304..4623)
                     /gene="uhpC"
                     /locus_tag="ACHJ12_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4988798.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4633..6135)
                     /gene="uhpB"
                     /locus_tag="ACHJ12_000971"
     CDS             complement(4633..6135)
                     /gene="uhpB"
                     /locus_tag="ACHJ12_000971"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4988799.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6135..6725)
                     /gene="uhpA"
                     /locus_tag="ACHJ12_000972"
     CDS             complement(6135..6725)
                     /gene="uhpA"
                     /locus_tag="ACHJ12_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4988800.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6800..7090)
                     /gene="ilvN"
                     /locus_tag="ACHJ12_000973"
     CDS             complement(6800..7090)
                     /gene="ilvN"
                     /locus_tag="ACHJ12_000973"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4988801.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7094..8782)
                     /gene="ilvB"
                     /locus_tag="ACHJ12_000974"
     CDS             complement(7094..8782)
                     /gene="ilvB"
                     /locus_tag="ACHJ12_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4988802.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8888..8986)
                     /gene="ivbL"
                     /locus_tag="ACHJ12_000975"
     CDS             complement(8888..8986)
                     /gene="ivbL"
                     /locus_tag="ACHJ12_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4988803.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9551..9640
                     /gene="tisB"
                     /locus_tag="ACHJ12_000976"
     CDS             9551..9640
                     /gene="tisB"
                     /locus_tag="ACHJ12_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4988804.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9903..9977)
                     /gene="ysdE"
                     /locus_tag="ACHJ12_000977"
     CDS             complement(9903..9977)
                     /gene="ysdE"
                     /locus_tag="ACHJ12_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4988805.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10059..11243
                     /gene="emrD"
                     /locus_tag="ACHJ12_000978"
     CDS             10059..11243
                     /gene="emrD"
                     /locus_tag="ACHJ12_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4988806.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11251..11748)
                     /gene="yidF"
                     /locus_tag="ACHJ12_000979"
     CDS             complement(11251..11748)
                     /gene="yidF"
                     /locus_tag="ACHJ12_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4988807.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QLRRDCQACDWHRLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11745..12107)
                     /gene="yidG"
                     /locus_tag="ACHJ12_000980"
     CDS             complement(11745..12107)
                     /gene="yidG"
                     /locus_tag="ACHJ12_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4988808.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12097..12444)
                     /gene="yidH"
                     /locus_tag="ACHJ12_000981"
     CDS             complement(12097..12444)
                     /gene="yidH"
                     /locus_tag="ACHJ12_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4988809.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12554..13003
                     /gene="yidI"
                     /locus_tag="ACHJ12_000982"
     CDS             12554..13003
                     /gene="yidI"
                     /locus_tag="ACHJ12_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4988810.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13050..14543)
                     /gene="yidJ"
                     /locus_tag="ACHJ12_000983"
     CDS             complement(13050..14543)
                     /gene="yidJ"
                     /locus_tag="ACHJ12_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4988811.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14540..16255)
                     /gene="yidK"
                     /locus_tag="ACHJ12_000984"
     CDS             complement(14540..16255)
                     /gene="yidK"
                     /locus_tag="ACHJ12_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4988812.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16422..17315
                     /gene="yidL"
                     /locus_tag="ACHJ12_000985"
     CDS             16422..17315
                     /gene="yidL"
                     /locus_tag="ACHJ12_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4988813.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17312..18126)
                     /locus_tag="ACHJ12_000986"
                     /pseudo
     CDS             complement(17312..18126)
                     /locus_tag="ACHJ12_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18126..19742)
                     /locus_tag="ACHJ12_000987"
                     /pseudo
     CDS             complement(18126..19742)
                     /locus_tag="ACHJ12_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            20038..20754
                     /gene="yidP"
                     /locus_tag="ACHJ12_000988"
     CDS             20038..20754
                     /gene="yidP"
                     /locus_tag="ACHJ12_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4988814.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20751..22412)
                     /gene="yidE"
                     /locus_tag="ACHJ12_000989"
     CDS             complement(20751..22412)
                     /gene="yidE"
                     /locus_tag="ACHJ12_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4988815.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22609..23037)
                     /gene="ibpB"
                     /locus_tag="ACHJ12_000990"
     CDS             complement(22609..23037)
                     /gene="ibpB"
                     /locus_tag="ACHJ12_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4988816.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEDTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23149..23562)
                     /gene="ibpA"
                     /locus_tag="ACHJ12_000991"
     CDS             complement(23149..23562)
                     /gene="ibpA"
                     /locus_tag="ACHJ12_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4988817.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23556..23628)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23868..24200
                     /gene="yidQ"
                     /locus_tag="ACHJ12_000992"
     CDS             23868..24200
                     /gene="yidQ"
                     /locus_tag="ACHJ12_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4988818.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24202..25416)
                     /gene="yidR"
                     /locus_tag="ACHJ12_000993"
     CDS             complement(24202..25416)
                     /gene="yidR"
                     /locus_tag="ACHJ12_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4988819.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25517..26581
                     /gene="cbrA"
                     /locus_tag="ACHJ12_000994"
     CDS             25517..26581
                     /gene="cbrA"
                     /locus_tag="ACHJ12_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4988820.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26578..27915)
                     /gene="dgoT"
                     /locus_tag="ACHJ12_000995"
     CDS             complement(26578..27915)
                     /gene="dgoT"
                     /locus_tag="ACHJ12_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988821.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27990..29138)
                     /gene="dgoD"
                     /locus_tag="ACHJ12_000996"
     CDS             complement(27990..29138)
                     /gene="dgoD"
                     /locus_tag="ACHJ12_000996"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO4988822.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29135..29752)
                     /gene="dgoA"
                     /locus_tag="ACHJ12_000997"
     CDS             complement(29135..29752)
                     /gene="dgoA"
                     /locus_tag="ACHJ12_000997"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO4988823.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29736..30614)
                     /gene="dgoK"
                     /locus_tag="ACHJ12_000998"
     CDS             complement(29736..30614)
                     /gene="dgoK"
                     /locus_tag="ACHJ12_000998"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO4988824.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30611..31300)
                     /gene="dgoR"
                     /locus_tag="ACHJ12_000999"
     CDS             complement(30611..31300)
                     /gene="dgoR"
                     /locus_tag="ACHJ12_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO4988825.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31578..32234
                     /gene="yidX"
                     /locus_tag="ACHJ12_001000"
     CDS             31578..32234
                     /gene="yidX"
                     /locus_tag="ACHJ12_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4988826.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32280..33092)
                     /gene="yidA"
                     /locus_tag="ACHJ12_001001"
     CDS             complement(32280..33092)
                     /gene="yidA"
                     /locus_tag="ACHJ12_001001"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4988827.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33207..33605)
                     /gene="yidB"
                     /locus_tag="ACHJ12_001002"
     CDS             complement(33207..33605)
                     /gene="yidB"
                     /locus_tag="ACHJ12_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4988828.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33845..36259)
                     /gene="gyrB"
                     /locus_tag="ACHJ12_001003"
     CDS             complement(33845..36259)
                     /gene="gyrB"
                     /locus_tag="ACHJ12_001003"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4988829.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     AVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36288..37361)
                     /gene="recF"
                     /locus_tag="ACHJ12_001004"
     CDS             complement(36288..37361)
                     /gene="recF"
                     /locus_tag="ACHJ12_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4988830.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37361..38461)
                     /gene="dnaN"
                     /locus_tag="ACHJ12_001005"
     CDS             complement(37361..38461)
                     /gene="dnaN"
                     /locus_tag="ACHJ12_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4988831.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38466..39869)
                     /gene="dnaA"
                     /locus_tag="ACHJ12_001006"
     CDS             complement(38466..39869)
                     /gene="dnaA"
                     /locus_tag="ACHJ12_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4988832.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40166..40246
                     /locus_tag="ACHJ12_001007"
     CDS             40166..40246
                     /locus_tag="ACHJ12_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988833.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40476..40616
                     /gene="rpmH"
                     /locus_tag="ACHJ12_001008"
     CDS             40476..40616
                     /gene="rpmH"
                     /locus_tag="ACHJ12_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4988834.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40633..40992
                     /gene="rnpA"
                     /locus_tag="ACHJ12_001009"
     CDS             40633..40992
                     /gene="rnpA"
                     /locus_tag="ACHJ12_001009"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4988835.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40956..41213
                     /gene="yidD"
                     /locus_tag="ACHJ12_001010"
     CDS             40956..41213
                     /gene="yidD"
                     /locus_tag="ACHJ12_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4988836.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41216..42862
                     /gene="yidC"
                     /locus_tag="ACHJ12_001011"
     CDS             41216..42862
                     /gene="yidC"
                     /locus_tag="ACHJ12_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4988837.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42968..44332
                     /gene="mnmE"
                     /locus_tag="ACHJ12_001012"
     CDS             42968..44332
                     /gene="mnmE"
                     /locus_tag="ACHJ12_001012"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4988838.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44575..44649
                     /gene="tnaC"
                     /locus_tag="ACHJ12_001013"
     CDS             44575..44649
                     /gene="tnaC"
                     /locus_tag="ACHJ12_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4988839.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44870..46285
                     /gene="tnaA"
                     /locus_tag="ACHJ12_001014"
     CDS             44870..46285
                     /gene="tnaA"
                     /locus_tag="ACHJ12_001014"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4988840.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46376..47623
                     /gene="tnaB"
                     /locus_tag="ACHJ12_001015"
     CDS             46376..47623
                     /gene="tnaB"
                     /locus_tag="ACHJ12_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4988841.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGIIVILCWFGNVFNVLPKFG"
     gene            47756..48931
                     /gene="mdtL"
                     /locus_tag="ACHJ12_001016"
     CDS             47756..48931
                     /gene="mdtL"
                     /locus_tag="ACHJ12_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4988842.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48906..49865
                     /gene="yidZ"
                     /locus_tag="ACHJ12_001017"
     CDS             48906..49865
                     /gene="yidZ"
                     /locus_tag="ACHJ12_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4988843.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELRRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            50022..50771
                     /gene="yieE"
                     /locus_tag="ACHJ12_001018"
     CDS             50022..50771
                     /gene="yieE"
                     /locus_tag="ACHJ12_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4988844.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            50793..51359
                     /gene="yieF"
                     /locus_tag="ACHJ12_001019"
                     /gene_synonym="chrR"
     CDS             50793..51359
                     /gene="yieF"
                     /locus_tag="ACHJ12_001019"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4988845.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51413..52750)
                     /gene="adeP"
                     /locus_tag="ACHJ12_001020"
     CDS             complement(51413..52750)
                     /gene="adeP"
                     /locus_tag="ACHJ12_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4988846.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            52917..53582
                     /gene="yieH"
                     /locus_tag="ACHJ12_001021"
     CDS             52917..53582
                     /gene="yieH"
                     /locus_tag="ACHJ12_001021"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4988847.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53649..54122
                     /locus_tag="ACHJ12_001022"
     CDS             53649..54122
                     /locus_tag="ACHJ12_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988848.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDNSCRKTDSLS"
     gene            complement(54205..54927)
                     /gene="yieK"
                     /locus_tag="ACHJ12_001023"
     CDS             complement(54205..54927)
                     /gene="yieK"
                     /locus_tag="ACHJ12_001023"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4988849.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(54942..56111)
                     /gene="yieL"
                     /locus_tag="ACHJ12_001024"
     CDS             complement(54942..56111)
                     /gene="yieL"
                     /locus_tag="ACHJ12_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4988850.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56138..57754)
                     /gene="bglH"
                     /locus_tag="ACHJ12_001025"
     CDS             complement(56138..57754)
                     /gene="bglH"
                     /locus_tag="ACHJ12_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO4988851.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(57840..59234)
                     /gene="bglB"
                     /locus_tag="ACHJ12_001026"
     CDS             complement(57840..59234)
                     /gene="bglB"
                     /locus_tag="ACHJ12_001026"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO4988852.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59253..61130)
                     /gene="bglF"
                     /locus_tag="ACHJ12_001027"
     CDS             complement(59253..61130)
                     /gene="bglF"
                     /locus_tag="ACHJ12_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO4988853.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61264..62100)
                     /gene="bglG"
                     /locus_tag="ACHJ12_001028"
     CDS             complement(61264..62100)
                     /gene="bglG"
                     /locus_tag="ACHJ12_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO4988854.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        15232 a        16560 c        15616 g        14907 t
ORIGIN      
        1 ccgggctgaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat
       61 tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg gcccaattgt
      121 gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc cggctttgca
      181 gcggattgat gctcgcggcg caacggcggt gccagggttt attgatgctc acctgcatat
      241 tgaatccagc atgatgacgc cggtcacttt tgaaaccgct accctgccgc gcggcctgac
      301 gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg gattcgcctg
      361 gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt
      421 acccgccctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc
      481 ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat
      541 tagcgggcag aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg
      601 tcactgcccg ggtttgggtg gtaaagaact taacgcctat attgctgcgg gtattgaaaa
      661 ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt
      721 gatgatccgc gaagggtccg ctgcccgcaa tctcaacgcg ctggcaccgt tgatcaacga
      781 atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg agatcgccca
      841 tgaagggcat atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccactgca
      901 tgtggcatat cgtgtcgcca gctggtcaac ggcgcgccac tttggtctga atcacctggg
      961 cttactggca cccggtaagc aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac
     1021 ggtgcagcag gtactggtga aaggcgagcc gattgatgcg caaaccttac aggcggaaga
     1081 gtcggcgaga ctggcacaat ccgctccgcc atatggcaac accattgccc gccagccagt
     1141 ttccgccagc gactttgccc tgcaatttac gcccggaaaa cgctatcggg tcattgacgt
     1201 catccataac gaattgatta cgcactccca ctccagcgtc tacagcgaaa atggttttga
     1261 tcgcgatgat gtgagcttta ttgccgtact tgagcgttac gggcaacggc tggctccgtc
     1321 ttgtggtttg cttggcggct ttggactgaa tgaaggtgcg ctggctgcga cggtcagcca
     1381 tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tggcggtcaa
     1441 tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt aacggccagg tacaaagtca
     1501 tctgccgtta cccattgccg ggctgatgag caccgacacg gcgcagtcgc tggcggaaca
     1561 aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat
     1621 tcagatggcg tttctttctc tgccagtgat ccccgcgcta aaactaacca gtcaggggct
     1681 atttgatggc gagaagtttg ccttcactac gctggaagtc acggaataat aaaaaaagcc
     1741 cggcgtcatg ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt
     1801 ttttctcgcg gcggattttg cgttcttcca ttaccgccac tatcgccatc aggcagatac
     1861 aaccaatcgc ggcgatatcc agcgcggcga aggtgcctgc ccagccggta aggccgaata
     1921 ccggcgtccc atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt
     1981 aagcaaaggt gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa
     2041 cagcagccac accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag
     2101 aagccagata gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca
     2161 gcgccagcgc gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc
     2221 cccacagcag cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca
     2281 ccgctttaga gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa
     2341 tacgtaccac atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca
     2401 gcacatactc aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct
     2461 cttcgctgat ctcctcgccg aacagttctt cagctttacc gaggccataa gattccgggg
     2521 agtcgctgcc gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga
     2581 taaacatgcc gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacacctg
     2641 ctgcgcctgc accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt
     2701 tacgacgcgg cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga
     2761 aaaagccgct taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca
     2821 tactggcact gaagcccagc atacaaatag cagagaggat cagcatgaac ggcaggaatt
     2881 gtttggtgtt tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga
     2941 tggagaaacc caggccgatc atccccagct gcgtcatgct caacccgtag gtcgaaatca
     3001 tatcgttctg cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa
     3061 agaccaccag gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg
     3121 gaaggtccag ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa
     3181 atgaatacct gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc
     3241 gtccaggcaa aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat
     3301 gcatcacgct tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat
     3361 cccggcggcg atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt
     3421 cgccagcggc caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt
     3481 cgccgcccct gccgcctctt tgtgggaaca ctctgccgcc gccatgccga ttaacatctg
     3541 cgggccaaag acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc
     3601 aaatggcatc agccacagcg agccgactga aagcaaaatt ccggcggcga aaatcaaatt
     3661 catcggccca cggttgccgt taaacaattt gtccgagccc caaccggcta ccagcgcacc
     3721 gataaatccg cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc
     3781 cagcgtctcg gacatataca aattgcccca gtcgttgatt gccgcccgga ccacatagac
     3841 cagcacatag caaaacgaaa gcagccagat atacggattc agcaacacat atttggtcag
     3901 gatctcttta cgcgtcaacc ctgccccttc ttgttgttga gcaatttcca gcgcgtcgtg
     3961 ccgccattca ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgta gccgccagca
     4021 gagaaaaatc cccacgacta tcgccataca accagcaatc atcatcccgg cacgccagcc
     4081 gtaatgcagc gcgctcgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc
     4141 cgtgttccat aatgcccacc aaccgccgcg ctcggtacgt gaataccagg ccgttaacag
     4201 acgcgcacac accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagcacggc
     4261 aaacgcccat agcgacgtcg agaagccaaa cagaatgttc atgatgcccg tggcgataag
     4321 ccctatcccc ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt
     4381 cgacacgccg taagtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag
     4441 cacgccgtta gcaaggattt ctggtaccgc ggcgttaaaa cttttccgcg tgaagtaaaa
     4501 cagcgcgtaa cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc
     4561 atcaatttca tatttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa
     4621 catcatcaaa ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag
     4681 acaggaaatg tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc
     4741 aaaaccttgt tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat
     4801 caaccgttca tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac
     4861 aatgttgttc agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa
     4921 cgctgattca tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat
     4981 ttcccgcatc agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg
     5041 taaccgaccc aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag
     5101 ctgcccgctc tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg
     5161 agtacgaata gcagtgatgg tctgaccgat atcatcatgc agctcacgcg ccacatcacg
     5221 acgcacgctc tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc
     5281 cttttgcagc gactggttaa gttcacgcaa ccgctggatg ccagcgccca gtaacaaccc
     5341 tgtcagactt tgcaccagca acgagagcaa taaatccacc ggatgatcgc gccaggtttg
     5401 actggcgatc agcgcgatgg cgttcatcaa cgtcgcgatc agcgcccctt gccaaccgta
     5461 gtgccaggcc agcgcgataa tcggcagcgc caggcagaat ggcgtaaagc gcgacagttc
     5521 gtccggcaat cccaactgga gccagagact aataacaaac aacagcaagt accagaccag
     5581 atgccgcccg cgccagttga tcggttgaga aaccagtgac gggccgagcg gcagccaggt
     5641 gttattggcg agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt
     5701 taaagtcagc aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg
     5761 caacaacgcc gccgccgtta acgccgcacc ctgtaacagc aaggtgcgcc agtcacgctg
     5821 atggcgatag cgcgagatca gcgccacggg cagtaacgtc agtaaactac cgatcatcaa
     5881 taacggaaaa tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc
     5941 gcccagcagc accggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc
     6001 aaacggaaat aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca
     6061 caggcaaaac catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga
     6121 gaacaacgtc ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg
     6181 acacccagtt tttccatcag gttggcgcga tggacgtgca ccgttttcgg cgacaagccc
     6241 agttcggcag caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctgccgt
     6301 tcgcgtttgg ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc
     6361 agataacagc cgcccgtggc aaccgtatgc accgccgcaa tcagttcgtc agggctacag
     6421 cgtttggaga gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgccggactg
     6481 tcatgaacag agagcattat tgtcgccata cctttcggca gctggcttag cagttccaga
     6541 ccggagatat cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc
     6601 cccgccagcg cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc
     6661 cccagcagct gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg
     6721 atcatggtct tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg
     6781 ttgttcaagc cttgagcggt tactgaaaaa acaccgcaat cttgttaaac atagtcggat
     6841 cggactgatt acgctgcact ttcaccacat cttccagctt atcgatttgg cttatcatct
     6901 gctccagacg ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa
     6961 tcggcagaca aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt
     7021 gggtcattac gcccggatgg ttgcgaacgg tgagctccag aatcacgttg tcatgagttg
     7081 tgttttgcat ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg
     7141 ggtaaacttt ttcttcggca tcaatgcgca catggatcag cgccgggcca gggcgattga
     7201 tgatttcctg caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc
     7261 cgaatccggc ggcaatctgc ataaagttga tttttcccgg ataggtggcg gcaaaaacgc
     7321 cttgctcgta gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca
     7381 gaatgatttt gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca
     7441 tcaggctgcc gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc
     7501 caatcgccgc aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc
     7561 gtgggcgatt gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg
     7621 tgataatcgc attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta
     7681 atggatcgca cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact
     7741 ggtgccactc tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat
     7801 caacatccgc ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga
     7861 tatcgacatg aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt
     7921 catcaaaacg cgcaccgagc actatcaaca aatccgcctc ctgcaaaata tagttggtgc
     7981 tgcgcacgcc gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcaacatgc
     8041 ccagcgccat taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc
     8101 gtgcgggcgc attgatcaca ccgccgccca gataaagcac cgggcgtttg gcagcgttaa
     8161 tcatcgccgc tgcgtcacga atgctttctt cgctaaaggc gggggcggcg gctttttctg
     8221 ccatagcggg ctgtgtttca atctcaaaaa ctgccgtttg cacatcctta ggaatgtcta
     8281 tccacaccgg gcctgggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg
     8341 ggagttcttc gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt
     8401 aggtgtccac ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccggtga
     8461 tgcaaatcag cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg
     8521 cacccggtcc gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccatcccct
     8581 gagcgataaa tcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt
     8641 ggcttaaggc atcgtaaaca ggcaggatag aaccgcccgg aatgcccgtc acaatcttaa
     8701 tgccctgctg ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtcg
     8761 atgttgtgcc cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg
     8821 tgcttaaacg aaaaaccccg cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt
     8881 ccagtcccta cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg
     8941 atagcgcagt tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg
     9001 gttttgtgtt taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt
     9061 ttcagcaagg gaaaattgag gggctgatca cgttttgtac tgaattgcag ataacaaaaa
     9121 accccgccgg agcgaggttt catcagccgc ctgcggctgg taaccgcaaa gcacactgta
     9181 ttatgtcaac actgaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt
     9241 tgacgtattt agggcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattact
     9301 gtttatttat acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg
     9361 catcgttacc cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc
     9421 ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt tctccttaag ataccgcgtg
     9481 cacaacagct ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca
     9541 ggagacgcgt atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc
     9601 actgcaactg cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca
     9661 aaggggagcg ggtaaccgct ccccttttat atttagcgtg cgggttggag ttgggggctt
     9721 cttctaccag cagaatgttt gcacctgcgc tgcctgtgcc cctcacccta gccctctccc
     9781 caaaggggcg aggggaccgt cccgtgcaat gtttagctca ttttctgaaa gggaacggtg
     9841 gtttccccct cgcccctttg gggagagggt tacagttcgc acgtaatccc tcccggcaac
     9901 atttattgcc gcttttgttt acatattctg ccgctaaaca attccccatt cctggcgtat
     9961 atctggctaa cattcatcaa tgtgatagat tcctctcccg catttatggg aatgcgtagt
    10021 gacttattct aattattttt ataaaagcat ccgtgataat gaaaaggcaa agaaacgtca
    10081 atttgttatt gatgttggta ttactcgtgg ccgtcggtca gatggcgcaa accatttata
    10141 ttccagctat tgccgatatg gcgcgcgatc tcaacgtccg tgaaggggcg gtgcagagcg
    10201 taatgggcgc ttatctgctg acttacggtg tctcacagct gttttatggc ccgatttccg
    10261 accgcgtggg ccgccgaccg gtgatcctcg tcggaatgtc catttttatg ctggcaacgc
    10321 tggtcgcggt cacgacctcc agtttgacgg tgttgattgc cgccagcgcg atgcagggga
    10381 tgggcaccgg cgttggcggc gtaatggcgc gtactttgcc gcgagattta tatgaacgga
    10441 cacagttgcg ccatgctaac agcctgttaa acatggggat tctcgtcagt ccgttgctcg
    10501 caccgctaat cggcggtctg ctggatacga tgtggaactg gcgcgcctgt tatctctttt
    10561 tgttggttct ttgtgctggt gtgaccttca gtatggcccg ctggatgccg gaaacgcgtc
    10621 cggtcgatgc accgcgcacg cgcctgctta ccagttataa aacgcttttc ggtaacagcg
    10681 gttttaactg ttatttgctg atgctgattg gcggtctggc cgggattgcc gcctttgaag
    10741 cctgctccgg cgtgctgatg ggcgcggtgt tagggctgag cagtatgacg gtcagtattt
    10801 tgtttattct gccgattccg gcagcgtttt ttggcgcatg gtttgccgga cgtcccaata
    10861 aacgcttctc cacgttaatg tggcagtcgg ttatctgctg cctgctggct ggcttgctga
    10921 tgtggatccc agactggttt ggcgtgatga atgtctggac gctgctcgtt cccgccgcgc
    10981 tgttcttttt cggtgccggg atgctgtttc cgctggcgac cagcggcgcg atggagccgt
    11041 tccccttcct ggcgggcacg gctggcgcgc tggtcggcgg tctgcaaaac attggttccg
    11101 gcgtgctggc gtcgctctct gcgatgttgc cgcaaaccgg tcagggcagc ctggggttat
    11161 tgatgacctt aatgggattg ttgatcgtgc tgtgctggct gccgctggcg acgcggatgt
    11221 cgcatcaggg gcagcccgtt taagcgcacg tcaccgcagc atcgtcatca gctccatggg
    11281 agaacgatgc tgctttatca gatcacgcat tacccgcata tgcggtgcgg agtaagaata
    11341 aaacgcctga tagcccgcac atagcacgct gtctggcgtc tcctcccgag cgtgcggaca
    11401 cagccgatgc cagtcgcacg cctggcagtc acggcgcagt tgcgggtttt cgctgccaca
    11461 ccatttttgt agggtagcgt caaaaagctc aatcgatata cgactgatat ctgcccgcac
    11521 ccaacactcg aacagggcaa ttaaaaattt cccccacagc tctgctgtca ccgaagcggc
    11581 cgtcagatcg ccccggtcat caaattcaag atggggaatg agctgtacgg agcggatgcc
    11641 ccgttgtttg aaattgtgat aaaaatcggc aggttgcaga gcatccttat actctacaac
    11701 cagcttatgc ctgtcctctt cagcatcaat gacctgactt cctgtcatgc gactctctca
    11761 ataaatacga taagttgatg tatatgcgtt acagcaaaca gtattgcgag agataacacc
    11821 gcgagggaga tcaaaaattt gtcacggact acgtgaaatt gggaaaaatc gctattcgtg
    11881 acatccatta aattgcgatt acgagtgtag tgccagagga tcagcgccac gatggcgagg
    11941 atgccaatcg aaatccagaa taacatgccc gcctggtgcc agttgtgttt gatagccaac
    12001 gccatcaacg cgccgtagcc cagcatggta cgaaaccacg ccagtgatgt acgctccggc
    12061 tgcagtcctg ggtcggcaat gcgtctggct ttgcggctat ccggcataca acaccagtcc
    12121 cataacgatc accgccacga ccatcaatat taaactgatg attaataagc tgttggtgta
    12181 aggcaggtcc tctttcagac gcatcgcttt ttcattgcgt aaccagcgca agtagccata
    12241 catcgctaac ccgccggaaa acagacacaa caacaacgcc agcagttcgc gaatgacggg
    12301 cgtggcgaaa tctggcgcaa gttgatcaag cccaaccccc gccgccagaa atcctagtgc
    12361 ggtacggatc cacgccagaa aagtacgctc attagccagc gagaagcggt aatcgggcgc
    12421 ttctccaagg cgggaaattt tcattgttgc tcctttgcaa tttcactaac gctctacctt
    12481 tagcggtttt tttttaagaa tttcgtccgc ccgtttcctg atatgctttc gccatccgct
    12541 acaaggaggt cagatgggta tcatagctca aaataaaatc agttcattgg gcatgctgtt
    12601 cggtgccatc gctttaatga tggggattat ccacttttca ttcggccctt tttccgcacc
    12661 accgccaacg tttgaaagca tcgtggcgga caaaacggct gaaattaaaa gaggtcttct
    12721 cgccggaata aaaggcgaaa aaattaccac cgtggagaaa aaagaggacg tagatgtcga
    12781 taaaatcctc gatcaaagcg gtatcgcact ggccattgct gcgctacttt gcgcatttat
    12841 tggcggtatg cgtaaggaaa atcgctgggg cattcgcggc gcgctggttt ttggcggcgg
    12901 gacgctggct ttccatacct tgctttttgg tatcggcatc gtgtgcagca ttttgctgat
    12961 attcctgatt ttttccttcc tgaccggcgg ttcactggtt taagcagcct gaataatgcc
    13021 cgccgggtgt tcatcagcgg gcattatcgt cagaacttct gttttttctc ttccactttc
    13081 actccttgcg tcggtaggcc ggtgtcatag tcacgtacca ccggcgaata gccatcttgt
    13141 gggcgtggac gaaatgcccc catccagcgc ggcagtgcat ctttacgcca cggacgcagg
    13201 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    13261 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    13321 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagtac cagtttaaag
    13381 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    13441 tagcggttaa attccaccat tacaccgcgt ggctctttca cggcaaggat attttccccc
    13501 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcgata
    13561 tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga
    13621 atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc
    13681 atttcgccgt gatcggaagt ataaatgacc cacgtatttt cacgttgctc tggcgttaag
    13741 gcattgatga cccgtccgat ttggtcatca acaaagtcat tacaggcaaa atagagcgga
    13801 tggtgataaa gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga
    13861 tgttccggtt tgttcgccag gtcatcctga gctttctcgc ccagatcgta gtaaaaatca
    13921 gcgtatttct ctaaatactc caccggacag gtgaacgggt gatgcggctc atcatacgaa
    13981 accaccatca ggaagggttc ctcggcgcgc gcgggctgtt gcagaaaatc taccgcccga
    14041 ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcgatat ggttcgcctg taaatcctca
    14101 acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaaggtag
    14161 ttcgccccat cgaaccagta atcagcgtcc cactccggcg gacactcgcc agtgccgaaa
    14221 tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta
    14281 aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg
    14341 gactggttag cgtagatacc ggtaaatagt ccggcgcgtg caggcgtaca aaccggtgaa
    14401 caggtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta
    14461 ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcatgatg
    14521 aacagaaaat tggggcgttt cataggctat ccttcgcgtc gggagtaaag gttacggctg
    14581 ggtcgtgacg atgccgccag ctgtcaaaaa tcaggtagac aatcactacg gcggcaatga
    14641 aataactgat catcgctaac cagcgcgtac cgtagccgcc gaattcagcc agcccggcat
    14701 agacgccaat catcgcgaac aggatgccaa ttgacgcgat cttgacgttt ctccacggtt
    14761 tcatgtccac cgcaaacgca tctttgaagg tgaacggcgt ggcgcgcggt ttgataaaac
    14821 cgatcaccag catcacgacc acgttgatgc agaacgtaca ggccagcaca tagaggaaat
    14881 ggaagtcgaa cttcaccaga tagttgatgg tgatgtagct gattatgcca atccccatcg
    14941 ccacttttgc cgccagcgcc gggatgcgcg ggaagaaaaa gcccataatg atgatggtaa
    15001 ccagcggcac gttgtaaatg ccgttgagct gtttcatcca gctatacagc ccctgcggcg
    15061 cgttggcgat ccacggcgcg accagcaccg aaacgatagc gataaagaaa ccaaatttgc
    15121 gcccgacggt gaccagctgc tgcggctcgg cattctggtt aatgatgcga cggtaaatac
    15181 ccatactgaa taacgtactg gcgctattca gaaagccgtt gaaggtactg atcaccgcac
    15241 caaataacac cgcgccgaag aaacccacca ttggcactgg cagaacgtta ttgaccagcg
    15301 tcgggtaggc catgtcggct ttcggtaaat cctgatacag atgaaacgca atcaaccctg
    15361 gcagcaccag taccagcggg tcgagcattt tcagcaccgc cgttaacagc gccccctttt
    15421 gcccttccgc caggcttttt gacgccagcg tgcgttgcac gatgccctga tttgtacacc
    15481 agtaaaaggt gttcaccaga atcaaaccgg taaatgccgc gccaatcggc aagggatcgg
    15541 tcgggccacc gattgagttt aatttctcgg cgtgaacggt ggtgagttgc tcaatgccct
    15601 gcataaagct gcccttgccc atcgcgatca ggccaaatac cggcaccatc aacccgccga
    15661 taaccagccc aataccgttg atggagtccg ccactgccat tgcgcgcagt ccgccgatca
    15721 ccgcatacaa aattcccgcc agaccaagca aaattaccag tagccagata gccgcaccgt
    15781 gagaaatctg tagcgattcc ccgacgtgaa acaggctgtt gagcgccaac gcgccggagt
    15841 agagaacaat cggcagaaag cagacgccgg tggcaatgag gaagcagaag tcgatgataa
    15901 tacgcgtcgt tttatcataa cgttcctcca gaaaatcggg gatggtggca atgccgcgct
    15961 tcagatagcg cggtaaaaag attagcgcga ggaagatcag cgtcaccgct gaagtcactt
    16021 cccagcccat caccgacatg ccgcttttgt aggcctgccc ggaaaggccg accagttgtt
    16081 ccgtggaaag gttggttagc attaacgaag cggcaataac cggcgctttt agtgaacgtc
    16141 cggcaagaaa atagccttgt tgcgatccgg tatctgtttt gcggaccttc caccaggtga
    16201 tcaccgccac cagcagcgta aaaccgacaa aactcaagat ttgtaacgaa ttcatctcat
    16261 agcccttatt tatgtttatg tcccggacgc atgaatatag ggagaggtaa aatgcgtcgg
    16321 gtcaggcagt cagattctta aaatacaaac gtcgtatccc tgaacggatt gttttctgtt
    16381 aagttcaggt tgtgagcata attcgccgga gggatattaa aatgaatgga aaattgcaaa
    16441 gttcagatgt aaaaaacgaa actccgtaca atattccctt attgattaac gaaaatgtga
    16501 tctccagcgg aatttctctg atctcgctct ggcataccta cgccgacgag cattaccggg
    16561 tgatctggcc gcgggacaag aagaaaccgc ttatcgccaa ctcatgggtt gcggtgtata
    16621 ccgtacaagg atgtgggaaa attcttttaa agaatggcga acaaataaca ctgcatggca
    16681 actgtattat attcttaaag ccaatggata ttcactctta tcactgtgaa ggtttagtct
    16741 gggaacagta ctggatggaa tttaccccca ccagtatgat ggatattccc gttggtcagc
    16801 aaagcgttat ttataatggc gaaatttata atcaggaact caccgaagtt gctgagttaa
    16861 taacttcacc agaagcaata aaaaataatc tggcagtcgc ttttctgacg aaaattattt
    16921 atcagtggat ttgtcttatg tacgcagatg gtaaaaaaga tccacaacgg cggcaaattg
    16981 aaaaattaat tgccacttta catgccagtc tgcaacaacg ctggagcgta gctgatatgg
    17041 ctgccacgat cccctgtagc gaagcctggt tgcgtcgtct gtttttacgc tataccggca
    17101 agacgccgaa agaatattac ctcgatgcgc gtctggatct ggcgctatcg ctattaaagc
    17161 aacaaggaaa ctcggttggc gaagtcgctg atacgctcaa cttcttcgac tcctttcatt
    17221 tcagcaaagc ctttaaacat aaatttggtt atgcgccgtc agccgtgcta aagaatacgg
    17281 accagcaccc aacggatgcc agtccacaca attaacgtaa ctctggccag tacgctttgt
    17341 tggcctccag caactcatcc agaatagctt tggcgaccga ggcgctcggt accgttttcg
    17401 acaacgtaat cgcctgccac aggtgctgat atgaacgctg ttcccaggcg tccacgacca
    17461 gtttttccac cgccacttgc tgactcatta accctttctg aaactgcggg atatcgccga
    17521 ccaccagtgg ttcaggccgt agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac
    17581 aatttgcgcc atccggcttt caataccgat tggcatgtca cagatgttga ggattttcgc
    17641 attcgggcgc agacgacgcg tggcttctgc gacaatggct gccgggttgg agtagttgag
    17701 catccaggca tttggtgaat atttttccat ataatccacc agttccagga cgccgccaat
    17761 ggaacgcatg ccgtacgcta ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg
    17821 gcgcagtggg attttttcat ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc
    17881 catgacaaaa tcaacgtcgc tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat
    17941 gtccggcgct ttctctttaa ggatgacttt acaggcttcg gcaatcactt cctgccgcgc
    18001 gccatcgtta tcataaaatt tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag
    18061 catcaacacg atgcctggcg taaaggtgct tccaccgcct gcgacaacca ctgagaattt
    18121 ggtcattata ctgcctccgt aatggcaaca ttttctgctg attgatgaga attaattaag
    18181 ctatcgagct gttcacgcag ctgggataca tgcagaccaa tgatcacctg aatggcatcg
    18241 ccactacgga agacgccgtg cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt
    18301 gacatgtcat gcagtgcaat gcgtaaacgc gtcgcgcaat tgttaatgct ggagatattg
    18361 ccgaccccgc ccagggcttg caggataccg gcagcctgat ccagctcttt ttttggctct
    18421 gccgcggtgg tttggcctcg cgaggctttg tattcggctt ttgagtagag tttcacttcc
    18481 gcatcttcac gtcccggcgt gcacatgttg aactgcagaa tcagtgtgcg gaaaaccacg
    18541 aagtacagca gggtaaagca caacccaatg gcgatttggg tcagcatcat atccgcgtgg
    18601 ttgctgaaca tcgggatcca gttttgcggt aaaacctggt caatcagacc tccgcccatg
    18661 ttgcccacca caccaaagag atacattacg gtcgacattg aggccgccag cacggcgtgt
    18721 accgcaaaca gcaacggtga aatgaacagg aaggtaaatt ccagcggttc ggtaattccc
    18781 accagcatgg cggttaaggt tgcgggaatc agcaagcccg ccacttttac ccgattttcc
    18841 ggtgcggcag tgaagtacat cgctaaagaa atgcccacgg caccaaagat ttttgagtta
    18901 ccgtgcaggg caaaaccgcc ttccgggaac aacgatttca gcggctcggc gctcaaactg
    18961 aactcttgca gatgctgcgc ccagtacatc tgaatgccgc cttcaacagc tgccggacca
    19021 aagataaact gtccgtagat gaagtggtgt aaaccggttg ggatcagaat acgttcgagg
    19081 aaggtgtaaa cccagacccc aagtgcaccc gccgaacgca ggaacgcttg cagagattca
    19141 atccccattt gtacttttgg ccagccgagc aacgtcagcc aggcacaggg gatcatcacc
    19201 aggaaggcga taatcaccac ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt
    19261 tttttatcga acagacggtt atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg
    19321 ataatgctgg tatcgagggt tttaatcccg gccatcattg tcagaccgct acctgccacc
    19381 gcgtcctgag tgaaatcgac gccgaagtag cttccccagg tcattcccat cgcgttgatg
    19441 aaatagttcc aggtcaggaa actcaccatc accgccagac aagcacgccc ctgcgcttgc
    19501 ttagcaaggc caatgggtaa accgacagca aaaatcagcg gcatattacg gaacaccgtc
    19561 caaccgccct cttcaataat gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt
    19621 gattacccga caaacatcgg gttttgcagc aagatggcaa gacccaccac aatcccggcg
    19681 aagggaaaca gcagcactgg cgtgaacatc gcgccgccaa agcgttgaat ttgactgagc
    19741 atttccacat cctcttttct caattctgta agtaggaagc ctgattggtt atgcggcagg
    19801 cctggagcga ggataggaag atgcgatcgg cagaacatga cgacccaatg ggattcgtga
    19861 tcgctttcat gcttttcgtt gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc
    19921 tgtgagcaca ggtacacagc atcgaacaca tctttaaaaa aaagatgttt tttcaatcga
    19981 ttaagcagaa cttgtgggcg cattacccgt gcttgcaggc aaaaaagaga tctagagatg
    20041 atctacaaaa gcattgcgga gcggttaaga attcgactta attccgcaga tttcacgcta
    20101 aatagccttc ttcccggtga aaaaaagttg gcggaagaat ttgcggtatc gcggatgacc
    20161 atccgtaaag ccattgacct gctggtagcg tggggattgg tggtccgccg ccacggcagc
    20221 ggcacttacc tggtgcgcaa agatgtactg catcaaaccg ccagcctgac cgggctggtg
    20281 gaggtgttaa aacggcaggg aaaaacggtc accagccagg tgctgatttt tgaaatcatg
    20341 cctgcgcctc cggccattgc cagccagtta cggattcaaa tcaacgagca gatctacttc
    20401 tcccgtcgcg ttcgttttgt ggaagggaaa ccgctgatgc tggaagacag ctatatgccg
    20461 gtaaaactgt tccgtaatct ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt
    20521 gaacaagagt gcgggatttt gattggcggt aattatgaaa gcctgacgcc agtgctcgcc
    20581 gatagactgc tggcgcggca aatgaaggta gcggaacaca cgccactgct gcggatcacc
    20641 tcgttgtcat atagcgagag cggggagttt ttgaattatt cagtgatgtt cagaaatgcc
    20701 agcgaatacc aggtggagta ccatttacgg cgactccacc cggaaaagag ttaaccgata
    20761 ctccagaaga gcaccgccag taattggggg gtgataatgc gcaggaacat cactaacgga
    20821 tagacagtgg cgtaagagag cgccgccgca ccgctggttg gatgaagatt attagcaaac
    20881 gccagcgccg gaggatcggt catggaacct gccagcatcc cgcacatggt caggtaattc
    20941 attttggcta acatccgcgc cagaatgcca acagtaatca gcggaacggc ggtgatcagg
    21001 gcaccataac caatccagct tagcccttcg ccattgacca gggtatttac aaaatcccca
    21061 ccagatttca gaccaacgac cgagaggaac agcacgatcc ccagctcccg cagcgcgagg
    21121 ttggcacttg gcggcataaa ccagtacagc ttgccgatac tgccgatacg cccgaggatc
    21181 aacgccataa tcagcggacc gcccgccagc cccagtttca acgcggccgg gaatcctggc
    21241 acaaagacgg gaatagaacc taacaatacg ccaagcccga tgccaataaa cactggcagc
    21301 atctgaacct gttgcagttt ttgctgcgca ttccccagca cattggcaac ggcatcaatt
    21361 gcggacggac gccccaccag gttgaggata tcgccgaact gcaggctgat atcgccactg
    21421 gcgaccagtt cgaccccggc acggttcagg cgcgagataa caacgtcata gcgttcttta
    21481 aagtgcaggt cgcgaatacg ttttccgagc acgttttcat tggtgaccac cacacgctcg
    21541 acgcgcaaat cagtgccttt cgtggacagc gaagtatcga cctcctgacc aatcaccagt
    21601 tgcgcattat gtaaatccgc tggctgcccc accagatgca gcaaatcgcc cagttggata
    21661 atggtatctg gcgaaggaac ttttagggtt tcttcgcgtt tcagacgcga gcagataatt
    21721 ttgtcgccgt tgagaatcgg tacatcttta atggctaaat catgcaggtt agggttctca
    21781 acgcgaatat tgatagtctt gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc
    21841 tgagcttctg tctcgacatt gacgcggaaa ataacccgca acatccacat ggtgaacaaa
    21901 atcccgcaaa tgccgaatgg atacgccatc gcgtaactca tccccatctg atcgaccatt
    21961 tccattggtg tacccaagtc gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg
    22021 gtaaccgcac cggagaaaat ccccagcact accggcagtg gaatatcaaa cagtttatgc
    22081 aggatggcgg taaccagacc accgatgatg acgatcagaa cagcaaacag gttgaggcgt
    22141 aatccggaga cgcgcaatga ggcaaagaag cccggcccta cctgaatccc gatagtataa
    22201 acgaacagga tcaggccaaa ttcctgaata acatgcagca tatcgctact taatgtcatc
    22261 cccgcctgag aaacaaaatg gccgacgatg atcccaccaa acagcacgcc gccaatacct
    22321 aatcctatgc cgcgaaattt gacattgccg ataaacaaac cgacgactgc caccaaagcc
    22381 agaatactga ccgttaatgc tatatcactc atcgtccatt tctcttgaga taacattttt
    22441 aattgctacg gattatggct gaatcggtga agcagatttg ccttatagcg cacattatca
    22501 cgggaaagaa cgtgccgaaa tatcttaaac agtcgaaatt taacgcattg ttgagtaagt
    22561 aagacaaaaa aagccccacc agaatggcgg ggcaaagaga atagctagtt agctatttaa
    22621 cgcgggacgt tcgctgatag cgatacgctg cgctgcgatg ggttcaggct cattacgaat
    22681 taaatcaata tgcagtaaac cgtttacgaa ggttgcgcca gagacttcca tattttcagc
    22741 cagcgtaaag ctcaggctaa atggctgatt cataagccct tgatgcagcc attttttctc
    22801 ttcttttggc tgctccggcg tgccttttac gctcaggcgc gtatcttcca gttgaatctc
    22861 taaatcttcc tgacggaaac ctgccagcgc aagggtaatg cggtagtggt tatcgtcgct
    22921 tttctcaatg ttgtacggcg ggaagctctg gctttcaccg gcgttttgca gtgcgttggc
    22981 cagtttgtca aaaccgatcc attgacgcat cagtggggat aaatcgaagt tacgcatagt
    23041 catttctcct tctaagaagc gagtaagtac ctgcaaatcc gaagattcgc atatgctccc
    23101 tgacggcgag catggagatg tcaggccgcg ccaggcggcc ttagggaatt agttgatttc
    23161 gatacggcgc ggttttttcg cttccggaat cacgcgttcg agatcgatat acagcaaacc
    23221 atttaccagg ttagcaccac gaacatgaat gttctcagct aactggaatt tgcgttcaaa
    23281 gttgcgttca gcgatgccct ggtacagata ggtgcgctct ttttgttcgt cggcgtgagc
    23341 acctttcacc accagcagat tatcctgggc ggtaatttcc agttcgctct cagcaaaacc
    23401 agccacagcg atagcaatgc ggtaatggtt ttcgtctacc agttcaacgt tatacggagg
    23461 gtagccgcca ttactctggc tctggttgtt ttctaagtgg ttaaacaaac ggtcaaatcc
    23521 aatagcagaa cggtaaagcg gggataaatc aaagttacgc ataatcaata gctcctgaaa
    23581 tcagcgagaa tgtaagacct tccacaatgg acaggtcagg tagccagaac acccatcagg
    23641 cgcgtcctca tcggctacga tgtaaaaatg ggtctggaaa tgaacttttc aagccttatc
    23701 agcggacttt tttttgcagt ttatggtcta ttgcatagac tgagggggca gcacgaaacg
    23761 ttaaagtgcg atagccgcca cagtgcgacg taatgatgat gttattttca gcaagggatc
    23821 gctataactc atcaggccaa cacccacgat caacagatga atttattatg ataagaaatg
    23881 tgttgttagc gttcatgata tgcagcggaa tgacattact cggaggatgc tccagcgtga
    23941 tgtcccatac cggggggaaa gaaggaacgt atcccggcac ccgcgccagc gccaccatga
    24001 ttggcgacga tgaaaccaac tggggcacta agtcgctggc gattctcgat atgccgttta
    24061 ccgctgtgat ggacacgctt ttactgccgt gggatgtgtt ccgcaaagac agctcggtgc
    24121 gctcgcgcgt tgaaaaaagc gaagccaacg cgcaagccac caatgcggtt attccaccgg
    24181 ccagaatgcc tgataactga tttagcgatc agtttcggtg atccacagct ggccgccttc
    24241 catccacgcc agccactgac catccggcga gaagaccacg gcgtccgcag aaggtggatt
    24301 ggcgtggttt tcggttaaat actcaacctc gccgctttgc gcatgggcac aagcaattcg
    24361 attatccagc acaaagccca accattctcc tgacggatgc cagttaaatg cagactgaat
    24421 atccgttttg ttatgggtta actggcgcgg ctcgccgccc tgtggcgaga taagccacag
    24481 ttgcacaatg ccgttatcat cacgcattaa aaacgcgatt tgcgtaccct gcggattaca
    24541 gcgcacccag tggcgcggga cgttgactaa ccccggataa gcccgatggt gggtaaaggt
    24601 taaacgtcgc tgcacgacgc cgcgcggtgg cgcgggcagg gtggtttccg ttccacttaa
    24661 cggcgcatca cctgccgctt tccagccagc ttcatcttgc ggtaactcaa cgataaacag
    24721 ctccggcact ttctcgccct ttggcgaaag tgtgtcgcca ataaacgcca gcgcgtgatt
    24781 tcctacccat ccttcttcat aagcacgatt gatttcatcg ctgccaggct gcggcgtggg
    24841 cgtggtttta ctcaccagca cgcaccagtg gctaccgctg tattcacgcg gatgctgctt
    24901 ttgtacgttg accgggccaa acggcgcagc aacaccgacg tttcgcaaat ccagcgccgg
    24961 atcgagttca tgcattacat ggtcgttata ggtaaagctc accctttcac cgttcgggct
    25021 aaagacatgc acatggctgc cgccgcgcag cgctcctggc gtatacggcg cggtaatatc
    25081 cattgcatcg agattgctca ccttgccgcc ttcagcaatg actccgcgtc gatgatggaa
    25141 atcgtaatgc catgtttcat caggattttc cgggccatgg atgaaaacat atttctctga
    25201 cttcggatga acggtcacca cgccgacgtg tgcgccctgt gacgcgcgat agataacctc
    25261 gacctcgccg gtatggatat tcacacgctc aatggtctcg ccggtaaacg acgccccaga
    25321 aggacgcacg tcaaatacca gccactggct gtcgggcgtc caggtattgg tattggtgag
    25381 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggg
    25441 tcccaccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
    25501 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
    25561 atcggcgttg gcgcgaaaat tagcaggcaa aatgcaggtg atcgcgctgg ataaaaagca
    25621 ccagtgtggt actgaaggtt tcagcaaacc ttgcggcggt ctgctggcac cggacgcgca
    25681 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtgatcgcca atccacagat
    25741 tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
    25801 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccaccagcgt
    25861 tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt gaggatgata aatggcatgt
    25921 catttttcgt gcagacggct gggagcaaca tattaccgcc cgctatctgg tcggtgccga
    25981 tggcgcaaac tcgatggtgc ggcgacatct ctacccggat catcaaatcc gtaaatatgt
    26041 cgctatccag cagtggttcg cggagaaaca tccggtgccg ttctactcct gcatctttga
    26101 taattcgata actaactgtt attcatggag tatcagcaaa gacggttatt ttatctttgg
    26161 cggtgcttat ccaatgaaag acggtcagac gcgtttcacg acgctgaaag agaaaatgag
    26221 cgcctttcag ttccagtttg gtaaggcggt gaaaagcgaa aaatgcacgg tgctgtttcc
    26281 ctcacgctgg caggattttg tctgcggtaa ggacaacgcc tttctgattg gcgaagcggc
    26341 aggatttatc agcgccagtt cgctggaggg gattagctat gcgctggata gcgcagacat
    26401 tctgcgtgcg gtgttactga agcagccaga gaagctcaat accgcttact ggcgcgccac
    26461 ccgcaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
    26521 ggctttaaga aagtggatta tgcgcagtgg tatggcgcat attccacagt tgaaagatta
    26581 gccaacgcgc ttcacatcgc ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
    26641 ggcggagata taaaccagtg caggtgcgaa accgtaaccc tgcgccaggt accccaccac
    26701 cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta aacacgccgc cggttaaacc
    26761 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt
    26821 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttagc
    26881 gcccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
    26941 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccgac
    27001 aaacgccgcg aggaatggca ccgtggtcat aaagcctgct ttcagcgccg tgattccttt
    27061 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgtag aagccaccgc
    27121 aaattgccca agataaacgc cgatcagttt acgatggaac accagtttcc agtctttggc
    27181 tgttaacggc tgacgcgcct ctttcttcac cggcgcatca ccatccacca gaccgccgcc
    27241 atcacgaatg taatccagtt cagctttgct gatacctttg gtcaggcgcg gcggctgata
    27301 aaccttaaac caaatcagcg accagataat gccgatacca ccagtgacaa tgaacaccca
    27361 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcaaaaacg ccagaccgac
    27421 aaactgacca gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct
    27481 ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcacg
    27541 cagaccaatt aatgacatta atcccgtggc aaagccctgg aataaagtcg ccactgacca
    27601 accaaatatc gcaataaaat aagtcacgcg agaacctacg cgatctaaaa accaaccgcc
    27661 ggggatctgg catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttgcgc
    27721 tttggtaata ccgaactctt cctgaatatg ggcggaagcc acggccaggt tagcgcggtc
    27781 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
    27841 cggttttgct gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc
    27901 gaagccgctc accatgccct gtaatttaca gagggttata tttttgtatt gctgttttag
    27961 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
    28021 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
    28081 tcgatttcca cgcctaagcc cggtttcgtt agcggtttaa agaagccgcc aaccatgctg
    28141 aagtcttctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
    28201 atactttgtt cctgaagtac ggcgttatag gaaacaaagt cgatatgcag gcaagccgcc
    28261 agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca catcataggc ttctgccatt
    28321 ccggcaattt tgtagcattc ggtaataccg cctgcatggg agaggtccgg ttgcagaatc
    28381 gaaataccac ctgcctccag cacgcgttta aaatcgaaac gtgagaacat gcgctcaccc
    28441 gccgccagtg gaatatgcgt ttgtgccgcc agtttcgggt agtattcggc ttgttccgcc
    28501 agcaccggct cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc
    28561 gccattggcg cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa
    28621 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
    28681 agccccagtt cttcacaacc gttcagttta aaggtatcga agccgatttc gcgcagggtt
    28741 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
    28801 ttgtcgcgaa ccaggccgcc catcagttgc cagaccggcg cattcagcac tttacctttg
    28861 atatcccata acgcctggtc gatcccggcg atggcgctca tcaggattgg accgccacga
    28921 taaaatccgg cgcgatacat cacttgccat aagtcattga tgcgcgatgg atcctgacca
    28981 atcaaatagt cacccagctc gtgaactgcc gcttccaccg tgcgggcgcg gccttcaatc
    29041 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    29101 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    29161 taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga ctgcccggcg cgatagagat
    29221 cgctgcctaa gcccgcccca gcacaacctg cgtctatcca ctgcgccagg ttttctggcg
    29281 tcacgccgcc aacggcaaag actgcgatgt ccgatggcaa taccgctttt aacgctttga
    29341 tgtattgcgg accaaaagcc gatgacggaa atattttcag cgcctgcgcg cccgcttcga
    29401 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca gacggtcatg ccgtagccca
    29461 ccgcacggcg gatcacttca ctatggatat tgggcgtaac gatgagctga cagcccatcc
    29521 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    29581 cgccatacgc atcaacgatg gcgggaatgc tttgctccca ttgtggggaa ttcagcggga
    29641 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    29701 gcttaatgcc gcgcaaaatg gcgatcagcg gaagtttagt ttgccactgc atgagcgatg
    29761 ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgtcacgtc gcaacccatc
    29821 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg
    29881 tgttgatggg tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag
    29941 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    30001 aaaagctgcg gcaatatggc gggcgcatta aggccgcgct caaggccagc cgcgaaggca
    30061 tcggcagagt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    30121 tgatgtaatt caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc
    30181 acccatttgc aatgcgtacc gggcataaca taaagagagg aaggagccag agctcgcgcg
    30241 ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt tatcgtcatg agagacgcat
    30301 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    30361 aaacgggcag gaacagataa atacggagca actttccagc cgacattgct gccgaccatt
    30421 cctgccatca ccactggcgt attttcttca cgccagtcgg tcgtgacttc tgctaacacc
    30481 gcagccggag attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctctccagg
    30541 cagtggtcgc cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg
    30601 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcgatc atggtaagcg
    30661 ccgcctgctc tgccgcatcg ccgtcctgat gccgtattgc atcgaacagc gccttatgtt
    30721 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    30781 gctgcagcga actgatcgca atgctaagtt gctgtaacac cgggttatgc accgactgca
    30841 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg
    30901 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaggtc gcgcgctctg
    30961 ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa
    31021 gccgtgggtc gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    31081 actggttacg cggtgccaca aacgccccgc gataacgttt catttcaatc agccgcttcg
    31141 ccatcagcga acggaacacc tcacggatga tgttgcgcga ggttgcaaac tcttcacaga
    31201 gctccgcctc agccggaagt ggcgagccag gtacgtattt gccgtgaacg atctgtttac
    31261 ccagcgtaat gacaatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    31321 atgttcttct ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt
    31381 gatgacgttc gcgtttgccg tgtgcgtaat gtagtacaaa cttatattgt tgtactacaa
    31441 tttagatcac aaaaagaaca atgcataaaa aaatgacatg cgtcgggcag aaatctgaaa
    31501 agggatatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact
    31561 cgtttaaagg attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttat
    31621 tcccactggc gctgatgctt tcaggctgta tctcgtatgc tctggtttcc cataccgcaa
    31681 agggtagttc aggaaagtat caatcgcagt cagacaccat cactgggcta tcgcaggcaa
    31741 aagatagtaa tggaacaaaa ggctatgttt ttgtagggga atcgctggat taccttatca
    31801 ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc agcacttaac cggcacaata
    31861 ttcaggttgc cgatgacgca agatttgttt taaatgcggg gaaaaagaaa tttaccggca
    31921 caatatcgct ttactactac tggaataacg aagaagaaaa ggcactggca acgcattatg
    31981 gttttgcctg tggtgttcaa cactgtacca ggtcactgga aaacctaaaa ggcacaatcc
    32041 atgagaaaaa taaaaacatg gattactcaa aggtgatggc gttctatcat ccgtttaaag
    32101 tgcgatttta tgaatactat tcacccagag gcattccgga tggtgtttcc gcagcattac
    32161 tgccagtgac tgttacgctg gacatcatta ctgcaccgct gcaatttctg gttgtatatg
    32221 cagtaaacca ataatcagta agcgggcaaa cgcgtttatg ctgtttgccc gtccactgat
    32281 taattcagca catacttctc aatagcaaac gccacgccat cttcaaggtt ggatttggtg
    32341 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    32401 gcatattcaa tcattgcgat atcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    32461 ttaataccta acacgtcggc cagtgatttc acccccgtac ctttgttaac gcgtttatcg
    32521 aggatttcga ggaagtacgg cgcacttttc agcacggtat atttctcttt cacttcctgc
    32581 ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat caatcatcat cactttcagg
    32641 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat ggtggcaacg
    32701 aaggattcat gcaccgtgta gtagctgata tcacggttgg cggtgtacag cgtggtgcgg
    32761 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acgatagtcg
    32821 tcatagctga gagcagtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    32881 ccgttataag taatgcagta gtcgcccggc tgttccatat gcagctcttt caggtagttg
    32941 tgcacacctg catacgggcg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    33001 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca
    33061 tccatatcga tagcaatgag tttgatagcc atgagttccc caggtagatt agttcctgac
    33121 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa gggggatgca
    33181 acgcgggttg catgctcccc atttgcttag cggaagagtt tccctttcag cagttccatg
    33241 cctgcggaaa gcagatcgtt attggcttgt ggagacactt caccttgcgg tgagagcgca
    33301 tcaataatct tcggcaattg ttctgccagt aaactggaag ctgtactggt atccacgcca
    33361 agtttttgtc caagatcgga caccgcattt gtgccgagcg ccgattccag ttgctcgcca
    33421 ctaaccgatt gattgccctg ttgattactc agccaggttg agagaatggc ccctaagccg
    33481 ccactttgca gtttttccag cagcacctga atgccgccct gctcctcaac ccaacttaaa
    33541 atagcctgat atttccccgc atcgcctttc agaaaggcac cgacaacttc atcaaaaagc
    33601 cccatgataa tcacctgtaa agcgttacgt gttgacccaa aaagtataga tttgcgggat
    33661 gataattgcg gattgcagaa ataaaaaggg cggagatgat ctccgccctt ttcttatagc
    33721 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaatcatgaa aattcaatac
    33781 attgcaagat tttcgtaggc ctgataagcg tagcgcatca ggcacgctcg catggttagc
    33841 gccattaaat atcgatattc gccgctttca gggcgttctc ttcaataaac gcacggcgcg
    33901 gttcaacggc gtcgcccatc agcgtggtga acaactggtc agcggcaatc gcatccttaa
    33961 cggtaacgcg cagcatacga cggctttccg ggtccatggt ggtttcccac agctgttccg
    34021 ggttcatctc gcccagacct ttataacgct ggatggagag gccgcgacgg gactctttca
    34081 ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg acgacgctcg ccacgttcga
    34141 taaacgcatc ttcttccagc aagccacgca gtttctcacc cagcgtgcag atacgacgat
    34201 attcgccacc ggtgataaac tcgtgatcca gcggatagtc agtatccaca ccgtgggtac
    34261 gcacgcgaac aatcggctcg aacaggtttt gttcggcatt ggtgtggaca tcaaacttcc
    34321 actggctgcc gtgctgttct ttgtcgttca gttcgctgac cagcgcgttc acccagcggg
    34381 taacggcctg ctcatcagag aggtcggctt ccgtcagcgt cggctgatag ataagctctt
    34441 tcagcattgc tttcggataa cgacgctcca tgcggttgat cattttctgc gtcgcgttgt
    34501 actcagacac caatttctct aacgcttcgc cagccaatgc cggtgcgctg gcgttggtgt
    34561 gcagcgttgc gccatccagc gcgatagaga tctggtactg atccatcgct tcgtcgtctt
    34621 taatgtactg ttcctgcttg cctttcttca ctttgtacag cggcggctga gcgatgtaga
    34681 cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa gaaggtcaac agcagcgtac
    34741 gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat gatgctgtga taacgcagtt
    34801 tgtccgggtt gtactcgtca cgaccgatac cacagccaag cgcggtgata agcgtcgcca
    34861 cttcctgaga agagagcatc ttatcgaagc gcgctttctc gacgttgagg attttaccct
    34921 tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg cttcgcagag ccgcccgcgg
    34981 agtccccttc caccaggtac agttcggaaa gcgccggatc gcgttcctgg cagtctgcca
    35041 gtttgcccgg caggcccgct aagtcgagcg cacctttacg gcgggtcatt tcacgcgcgc
    35101 gacgcgccgc ttcacgggca cgggcagcat cgataatttt gccaaccacg attttcgcgt
    35161 cggttgggtt ttccagcagg tattctgcca gcagttcgtt catctgctgt tcaaccgctg
    35221 atttcacctc agaagaaacc agtttgtctt tggtctggga ggagaatttc gggtccggca
    35281 ctttcacgga aacgaccgca atcaggcctt cacgcgcatc gtcaccggtg gcgctaactt
    35341 tggctttttt gctgtagcct tctttgtcca tgtaggcgtt cagggtacgg gtcatcgccg
    35401 cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg aatgttgttg gtaaagcagt
    35461 agatgttttc ctggaagcca tcgttccact gcaacgccac ttcgacgcca ataccgtctt
    35521 tttcggtgga gaagtagaag atattcgggt ggatcggcgt tttgttcttg ttcagatatt
    35581 caacgaacgc cttgatgccg ccttcatagt ggaagtggtc ttctttgccg tcacgcttgt
    35641 cgcgcagacg aatggaaacg ccggagttga ggaacgacaa ctcacgcaga cgtttcgcca
    35701 gaatttcata ttcgaactcg gtcacattgg tgaaggtttc gaggctgggc cagaaacgca
    35761 ccatggtgcc ggttttttca gtctcgccgg taaccgccag cggagcctgc ggtacaccgt
    35821 gttcgtagat ctgacggtga attttaccct cgcgctggat aaccagctcc agtttttgcg
    35881 acagggcgtt tacaaccgaa acaccaacgc cgtgcagacc gccggacact ttataggagt
    35941 tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat cacttccgcc gccgatacgc
    36001 cctcttccgg gtgaataccg gtcggaatgc cgcgcccatc atcctgtacg gagacagagt
    36061 tatcggcgtg aatggtgacg ataatttctt tacagtgacc cgcgagcgct tcgtcgatag
    36121 cgttatctac cacctcgaat accatgtggt gcagaccggt gccgtcatcc gtgtcgccga
    36181 tatacatacc cgggcgctta cgcaccgcat ccagcccttt caggactttg atactggagg
    36241 agtcataaga attcgacatc aacgtttctc gctcatttat acttgggtta atccgttatt
    36301 ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg acatgtctat aacgtgttca
    36361 gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt ttaagcggct ggcaagcagc
    36421 ccgcgacgct catcatcaag ctcagaggca aaatcatcta tcaggtagag acaccgccgc
    36481 ccgctttcac gggtgaggaa ctctccttgc gccagacgta aggcgcacat caacagctta
    36541 agctgcccac gcgataaggt atcttccacc ggcgcaccgt cggcgcgaat gcgtaagtcc
    36601 gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat cgcgttcaaa attacgttcc
    36661 agcacctcag catattctgt ctctttctcc cagccgcgct ggaaagagaa agtcagagaa
    36721 aactcaggga gaaattgctt acaggtatca gccatatcgg ccgcgatacc ggcgctatac
    36781 tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca gttctttatc ccacgggcgt
    36841 agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc gctgcttgag caatcgcttg
    36901 agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa agcatcccca gtcgaggaat
    36961 gctcttctgt atttggggcc gccgttgagt aaagtaaacc cttcaggcgt tatcagctgc
    37021 atcggcatca ggtgcgccaa ctccgcaacc ttatgcccgt cagtaccgtc aatacggact
    37081 ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg tctcgcgctc ttcgccctgt
    37141 aatcgcccat ggagaacaaa cgcctcctgc tcatggcgaa tgacgcgacc aatctgcaaa
    37201 ctgcgaaacg cccgaccatg gccgagcgta tagatggctt ccagcacgct ggttttgcca
    37261 ctgccgttgg cacctaccag aaagttaaag ccgggagata aagcgagatc cgcggtttca
    37321 atgttgcgga aatcgcggat caacaagcgg gtgagggaca ttacagtctc attggcatga
    37381 caacataggc agccgactgt gatgcggcat cttcaatctg cacgctggaa accgaatcgg
    37441 tcagcatcat gcggacgttt tcacatttca gcgcgttcag gacatccagc acatagctga
    37501 cgttgaagcc gatttccatc tccgcaccgc tataggtaac gtcgaggatc tcttccgctt
    37561 cttcctgttc cgggttgttg gcggtgattt tcagctggtt ttcgctgaca tacaggcgca
    37621 cgccgcggaa tttctcgtta gagagaattg ccgcgcgagc aaacgcctgc ttgaggagat
    37681 cgcagccagc ttccagatgt ttgtccgggt tcttcggcag aacgcgacgg taatccggga
    37741 agcgaccatc caccagtttg gaggtgaaga taaagtcgcc aacgtgggcg cgaatgttgt
    37801 tgctgccaat ctgtacgcgc agcggattgt cgccgccgtc gagcatacgc atcagttcaa
    37861 tcacgccttt acgcggcacg atcaccgaat ggcttggcaa agattgacca attggcattg
    37921 aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg cagttcttca ccttcggttt
    37981 caaacagcat accatttaag taatagcgaa cgtcctgatg cgccatcgaa aactgggtcg
    38041 cttcaatcag acgcttcatc gttgcctgcg gcagggtaaa ttcgacttca ctctgccagt
    38101 catcgaggtt cgggaaatcc gccgctggca gggtagacag cgaaaaacgg ctacgcccgg
    38161 agcgcaccag catccgctca ccttccagct gcacggcaat ttccgcgcct tcaggcagac
    38221 cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt cgctcctggc tcgtgtggct
    38281 gaaccagcgc aacgcgtgcc accatttcca tctcgagatc agtaccggtc agcgacaacg
    38341 taccgtcagc aacctgtaac agcagattac cgagaatcgg tagcgtagga cgaccaccta
    38401 acggaccgct cacctgttgt agcggtttta ataaatgctc acgttctacg gtaaatttca
    38461 taggtttacg atgacaatgt tctgattaaa tttgaaaaat cttctttgat atcgtggctc
    38521 tcttcacgca actgctcgat cttacggcag gcatgaagca ccgtcgtgtg gtcacgacca
    38581 ccaaacgcat cgccaatctc cggcagactg tggttagtca gctctttcgc cagcgccatc
    38641 gccatctggc gcggacgcgc caccgagcgg gatcgacgct tggaaaggag atccgcgact
    38701 ttgatcttgt agtactccgc caccgtcttc tgaatattgt cgatggtgac cagtttttcc
    38761 tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt cgatggtgat cgcccgtccg
    38821 gtaaagttgg cattggcaat gacgcggttc agcgcccctt ccagctcacg tacgttagat
    38881 cgtagacgct tggcgataaa gaacgccact tcgcccggca aacgaatgtc gttttcgtcg
    38941 gcctttttca tcaggatcgc cacacgggtt tccagctctg gcggttcgat cgccacagtc
    39001 agtccccaac cgaagcggga tttcaaacga tcctcaacgc cgttgatctc tttcggatag
    39061 cgatccgagg tgagaatgat ctgttgatta ccttccagca gggcgttgaa ggtatggaaa
    39121 aactcttcct gagatcgttc tttattagca aaaaactgaa tatcgtcgat cagcagtgca
    39181 tctacggaac ggtagtagcg tttaaactct tcgatcgcgt tgttttgcag ggctttaacc
    39241 atgtcctgaa caaagcgctc ggagtgcata taaaccactt tggcattcgg cttgcgcgcc
    39301 ataatgccgt tacccaccgc atgcagcagg tgagttttac ccagacccgt gccgccataa
    39361 aggaacaacg ggttataggc accgccaggg ttatccgcca cctggcgagc cgccgcgcgc
    39421 gccagttggt tagatttacc ttcaacgaag ttatcaaacg tgtgtttgac gtttacgtta
    39481 gaacgatagg tcggttctgc cggggccggg acgttatccc aacctgagcg cgtagaaggc
    39541 gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg ccgcgacgtt gctcgtcact
    39601 gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt caaaacgcag ctgtggggca
    39661 tccgctccgc agaaactggt tagcagtcca ttgatattat taaggtactt gtcccgtacc
    39721 caatcgagga caaaacggtt tggcgcgtac agggccagcg tgttatcgct cagttccgcc
    39781 tgcaatgggc gtatccacat actgaattct gtggctggta actcatcctg caatcgggca
    39841 agacactgct gccaaagcga aagtgacacg gcggactcca ctcgaacaaa agtcgataat
    39901 gactaaggct gaaacattca tgattgttga cgtacgtcga aaagaccctg cttgagggtg
    39961 acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga tcctgcaaaa cgatcgggac
    40021 cgcggatcat agcctaaact gcgcaagaga tcttctgttt ctcacagatt ttcccgattt
    40081 atccacagga ctttccagaa ctcgctaagt gtaaacgatc ctgccgcgag gcgggcacga
    40141 tttacgccgc atattggaaa atttaatgac catagacaaa aattggctta atcgatctaa
    40201 taaagatcca ggacgatcct tgcgctttac ccatcagccc gtataatcct ccacccggcg
    40261 cgccatgctg gtttccactg gtgtgaggtc gtacattttc cctgcgaaaa ggtgcggaaa
    40321 agcgcggtaa ataaggaaag agaattgact ccggagtgta caattattac aatccggcct
    40381 ctttaatcac ccatggcttc ggtgtccatc gtttcatttt tcggcggata tccaataaag
    40441 ccattgaatt tattcaagtt taggtagaaa tcgccatgaa acgcactttt caaccgtctg
    40501 tactgaagcg caaccgttct cacggcttcc gtgctcgtat ggctactaaa aatggtcgtc
    40561 aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct gaccgtttct aagtaataaa
    40621 gctaacccct gagtggttaa gctcgcattt cccagggagt tacgcttgtt aactcccagt
    40681 caattcacat tcgtcttcca gcagccacaa cgggctggca cgccgcaaat taccattctc
    40741 ggccgcctga attcgctggg gcatccccgt atcggtctta cagtcgccaa gaaaaacgtt
    40801 cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc gtgaaagctt ccgtctgcgc
    40861 caacatgaac tcccggctat ggatttcgtg gtggtggcga aaaaaggggt tgccgacctc
    40921 gataaccgtg ctctctcgga agcgttggaa aaattatggc gccgccactg tcgcctggct
    40981 cgcgggtcct gatagccctc attcgggtct atcaacgcct gattagtccg ctactcgggc
    41041 cgcattgtcg tttcactcca acctgttcaa gctacggaat tgaggcattg cgcaggtttg
    41101 gagtgataaa aggcagttgg ttgacggtga aacgcgtatt aaaatgccac cctttacacc
    41161 ctggtggtga cgatcccgtc ccgcccggac catttgatac cagagaacac taacgatgga
    41221 ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg tctttcatga tctggcaagc
    41281 ctgggagcag gataaaaacc cgcaacctca ggcccaacag accacgcaga caacgaccac
    41341 cgcagcgggt agcgccgccg accagggcgt accggccagt ggccagggga aactgatctc
    41401 ggttaagacc gacgtgcttg atctgaccat caacacccgt ggtggtgatg ttgagcaagc
    41461 tctgctgcct gcttacccga aagagctgaa ctctacccag ccgttccagc tgctggaaac
    41521 ttcaccgcag tttatttatc aggcacagag cggtctgacc ggtcgtgatg gcccggataa
    41581 cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa gacgcttatg tgctggctga
    41641 aggtcaaaac gaactgcagg tgccgatgac gtataccgac gcggcaggca acacgtttac
    41701 caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac gtcaactaca acgtgcagaa
    41761 cgctggcgag aaaccgctgg aaatctccac ctttggtcag ttgaagcaat ccatcactct
    41821 gccaccgcat ctcgataccg gaagcagcaa cttcgcactg cacaccttcc gcggcgcggc
    41881 gtactccacg cctgacgaga agtacgagaa atacaagttc gataccattg ccgataacga
    41941 aaacctgaac atctcttcga aaggtggttg ggtggcgatg ctgcaacagt atttcgcgac
    42001 ggcgtggatc ccgcataacg acggtaccaa caacttctat accgctaatc tgggtaacgg
    42061 catcgccgct atcggctata aatctcagcc ggtactggtt cagcctggtc agactggcgc
    42121 gatgaacagc accctgtggg ttggcccgga aatccaggac aaaatggcag ctgttgctcc
    42181 gcacctggat ctgaccgttg attacggttg gttgtggttc atctctcagc cgctgttcaa
    42241 actgctgaaa tggatccata gctttgtggg taactggggc ttctccatta tcatcatcac
    42301 ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg cagtacacct ccatggcgaa
    42361 gatgcgtatg ctgcagccga agattcaggc aatgcgtgag cgtctgggcg atgacaaaca
    42421 gcgtatcagc caggaaatga tggcgctgta caaagctgag aaggttaacc cgctgggcgg
    42481 ctgcttcccg ctgctgatcc agatgccaat cttcctggcg ttgtactaca tgctgatggg
    42541 ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc cacgacctgt cggcacagga
    42601 cccgtactac atcttgccga tcctgatggg cgtaacgatg ttcttcattc agaagatgtc
    42661 gccgaccaca gtgaccgacc cgatgcagca gaagatcatg acctttatgc cggtcatctt
    42721 caccgtgttc ttcctgtggt tcccgtcagg tctggtgcta tactatatcg tcagcaacct
    42781 ggtaaccatt attcagcagc agctgattta ccgtggtctg gaaaaacgtg gcctgcatag
    42841 ccgcgagaag aaaaaatcct gattcggtga gttttcgcta aaataagggc ggtcagttga
    42901 ccgccttttt tcttttcgta gggcggataa gcaccgcgca tccgccacac aaagcaacag
    42961 gaacatcatg agcgataatg acactatcgt agcccaggcc acgcctccgg gacgtggcgg
    43021 cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa gttgccgaaa ccgtgctggg
    43081 taaactacct aagccgcgct acgccgatta tcttccgttt aaagacgccg acggcagcgt
    43141 gctcgatcag gggattgcgc tatggttccc tggcccgaac tcgttcaccg gcgaagatgt
    43201 gctggaactg caaggtcatg gcggtccggt gatcctcgac ctgctgttaa aacgcattct
    43261 gaccattccc ggcctgcgga ttgctcgccc tggtgagttt tccgaacgcg cgtttcttaa
    43321 cgataaactc gacttagccc aggccgaggc gattgccgat cttatcgacg ccagttcgga
    43381 acaggcggcc cgttcggcac ttaactcgct gcaaggcgca ttctccgcac gggtcaacca
    43441 tctggtggaa gccctcaccc acttgcgcat ttacgtcgaa gcggcaattg atttccctga
    43501 tgaagagatc gatttcctct ccgacggcaa aattgaagcc cagcttaatg acgtgattgc
    43561 cgaactcgat gcagtgcgtg ctgaagcacg tcagggtagt ttgttgcgcg aagggatgaa
    43621 ggtggtgatt gccggacgtc ctaacgccgg taaatcgagc ctgttaaacg cgctggcggg
    43681 ccgtgaagca gcaatcgtaa ctgatatcgc cggaaccaca cgtgacgtgc tgcgtgagca
    43741 tatccacatt gacggaatgc cgctgcatat catcgatacc gctgggctac gtgaagccag
    43801 tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag gaaattgaac aggccgaccg
    43861 cgtgctgttt atggtcgatg gcaccacgac agacgccgtt gatccggcag agatctggcc
    43921 ggaatttatc gcccgtctgc cagcgaaact gccgatcacc gtggtgcgca ataaagccga
    43981 tatcaccggc gaaacgctgg gaatgagtga agtgaacggt cacgcgttaa ttcgtctctc
    44041 ggcaaggact ggtgaaggtg tggatgtgct gcgtaaccat ctcaaacaga gcatgggctt
    44101 tgacaccaac atggaaggcg gcttcctggc gcgccgtcgc cacctacagg cgctggaaca
    44161 ggcggcagag catttgcagc aaggtaaagc gcaactgtta ggtgcctggg cgggcgaact
    44221 gctggcggaa gagttgcgcc tggcacagca gaacttaagc gaaatcaccg gggaatttac
    44281 ttctgatgac ctgctggggc ggattttctc cagcttctgt attggtaagt aaccgcgctt
    44341 acgaagccgt attctgactg tcagatgcgg cttcgcttca ttgttagcac tcctgttatt
    44401 cctcaaccct ttttttaaac attaaaattc ttacgtaatt tataatctta aaaaaaagca
    44461 tttaatattg ctccccgaac gattgtgatt cgattcacat ttaaacaatt ttagaataga
    44521 caaaattctt gagtattata ctgtagcctc gtgtctttcg aggataagtg cattatgaat
    44581 atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg acaataagat tgtcgatcac
    44641 cgcccttaat ttacccttct gtagccatca ccagagccaa accgattaga ttcaatgtga
    44701 acgattagtt tgctatatct taattttgcc ttttgcaaag gccatctctc gtttatttac
    44761 ttattttagt aaatgatggt gcttgtatat atacatggcg aattaatgga tattgcagat
    44821 gtaatattca cagggatcac tgtaattaaa ataaatgaag gattatgtaa tggaaaactt
    44881 taaacatctc cctgaaccgt tccgcattcg tgttattgag ccagtaaaac gtactactcg
    44941 cgcttatcgt gaagaagcaa ttattaaatc cggtatgaac ccgttcctgc tggatagcga
    45001 agatgtgttt atcgatttac tgaccgacag cggcaccggg gcggtaaccc aaagtatgca
    45061 ggcagcgatg atgcgcggcg acgaagccta cagcggcagc cgcagctact atgcgttagc
    45121 cgagtcagtg aaaaatatct ttggttatca atacaccatt ccgactcacc agggccgtgg
    45181 cgcagagcaa atttatattc cggtactgat taaaaaacgc gagcaggaaa aaggcctgga
    45241 tcgcagcaaa atggtggcat tctctaacta tttctttgat accacgcagg gccatagcca
    45301 gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata ctggcgtgcg
    45361 ttacgacttt aaaggtaact ttgaccttga gggattagaa cgcggtattg aagaagttgg
    45421 cccgaataac gtgccgtata tcgttgcaac catcaccagt aactctgcag gtggtcagcc
    45481 ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg atattccggt
    45541 ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc atcaagcagc gtgaagcaga
    45601 atacaaagac tggaccatcg agcagatcac ccgcgaaacc tacaaatatg ccgatatgct
    45661 ggcgatgtcc gccaagaaag atgcgatggt accgatgggc ggcttgctgt gcatgaaaga
    45721 cgacagcttc tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc aggaagggtt
    45781 cccgacatat ggcggcctgg aaggcggcgc gatggagcgt ctggcggtag gtctgtatga
    45841 cggcatgaat ctcgactggc tggcttatcg tatcgcgcag gtacagtatc tggtcgatgg
    45901 tctggaagag attggcgttg tctgccagca ggcgggcggt cacgcggcat tcgttgatgc
    45961 cggtaaactg ctgccgcata tcccggcaga ccagttcccg gcacaggcgc tggcgtgcga
    46021 gctgtataaa gtcgccggta tccgtgcggt agaaattggc tctttcctgt taggccgcga
    46081 tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg
    46141 cgcaacatat actcaaacac atatggactt cattattgaa gcctttaaac atgtgaaaga
    46201 gaacgcggcg aatattaaag gattaacctt tacctacgaa ccaaaagtat tgcgtcactt
    46261 caccgcaaaa ctgaaagaag tttaattaat actacagagt ggctataagg atgttagcca
    46321 ctctcttacc ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac
    46381 tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt atggttatag caggtacagt
    46441 aattggtgga ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg
    46501 tgcctttatc cttatcattg cctggttttc aatgcttcat tccgggttat tgttattaga
    46561 agcaaattta aattatcctg tcggctccag ttttaacacc atcaccaaag atttaatcgg
    46621 taacacctgg aacattatca gcggtattac cgttgccttc gttctctata tcctcactta
    46681 tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta
    46741 tcacgctaat ccacgtattg tcgggatctg cacagccatt ttcgttgcca gcgtattgtg
    46801 gataagttcg ttagccgcca gtcgcattac ctcattgttc ctcgggctga agattatctc
    46861 ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat tactccatcc tgcgcgatgc
    46921 caccagcacc actgcgggaa cgtcttactt cccgtatatc tttatggctt tgccggtgtg
    46981 tctggcgtca tttggtttcc acggcaatat tcccagcctg attatttgct atggaaaacg
    47041 caaagataag ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg tgatttatct
    47101 cttctggctc tattgcacga tggggaatat tccgcgcgaa agctttaagg cgataatctc
    47161 ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat
    47221 tatcgagttt tgcctgctgg tgttctctaa cttagctgtt gccagttcgt tctttggtgt
    47281 cacgctgggg ttgttcgatt atctggcgga cctgtttaag attgataact cccacggcgg
    47341 gcgtttcaaa accgtgctgt taaccttcct gccacctgcg ttgttgtatc tgatcttccc
    47401 gaacggcttt atttacggga tcggcggtgc cgggctgtgc gccaccatct gggcggtcat
    47461 tattcccgca gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga tgttcacggt
    47521 ctggggcggc aatcttattc cggcgattgt cattctcttt ggtataatcg taattttatg
    47581 ctggttcggt aacgtcttta acgtgttacc taaatttggc tgatcccttc aagaagccag
    47641 ccatttcgct ggcttcttgc ctgtcaggaa atctcttttg tccaaatggc aactcgcctg
    47701 attctccttc accacgtatg ctttgcgtca ccttactatc aggacgcttt agcccatgtc
    47761 ccgctttttg atttgtagtt ttgccctggt tttactttat cccgccggga ttgatatgta
    47821 cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc agcgaagcgc agttgcatat
    47881 tgcgttctcc gtatatctgg cggggatggc agctgcgatg ttatttgccg gtaaagtggc
    47941 cgatcgttca gggagaaagc cggtcgccat acccggcgcg gcgctattta ttattgcctc
    48001 ggtgttctgt tcactggctg aaaccagcac gttatttctt gcaggccgat ttctacaggg
    48061 gttgggcgca ggctgttgtt acgtagtggc gttcgccatt ttgcgcgaca cgctggatga
    48121 tcgacgtcgg gctaaagtgc tgtcattact caacggtatt acctgcatca ttccggtgtt
    48181 agcgccagtg ctcggacatc tgattatgct taaattcccg tggcagagtc tgttctgggc
    48241 gatggcaatg atgggcatcg cggtactgat gttgtctttg tttattttaa aagaaacgcg
    48301 cccagcgtcc cccgctgctt cggacaaacc acgagaaaat agcgagtcgc tgcttaatcg
    48361 gtttttcctc agccgtgttg ttatcaccac cctcagcgtt tcggtgatcc tcactttcgt
    48421 caatacatcg ccggtattgc tgatggaaat catgggtttt gagcgcggag aatacgccac
    48481 cattatggcg ttgaccgctg gcgtcagcat gaccgtttca ttctccacgc catttgcgct
    48541 gggaattttt aagccacgta cgttgatgat cacctcgcag gtgttattcc ttgcggcggg
    48601 gatcaccctt gccgtttcac cttcccatgc ggtttctctg tttggtatca cgctgatttg
    48661 cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag gcgttaggac cattttcatt
    48721 acgcgcgggc gtagccagct cgaccttagg tattgcgcag gtttgcggtt cgtcactgtg
    48781 gatttggctg gcagcggtgg ttggtatcgg cgcatggaat atgctgatcg ggattctgat
    48841 tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc gcgcctggac gccccgttgc
    48901 cgctcatgaa gaaatccatc accacgcttg atctcaatct gctgctctgt ctgcaactgc
    48961 tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat gaacgtgacc ccttcagcgg
    49021 tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga cccgctcttt gtgaactcac
    49081 cgctgggcct gtcgcccaca ccgctaatgg tcagcatgga gcaaaatctg gcggaatgga
    49141 tgcaaatgag caatctgctg ctggataaac cgcaccacca gacaccgcgc ggcctgaagt
    49201 ttgagttggc ggcggaatcg ccgctgatga tgatcatgct taatgcgctg tcgaaacgga
    49261 tctaccaacg ttacccgcag gcgaccatca aactacgtaa ctgggattac gattccttag
    49321 atgccattac tcgtggtgaa gtggatattg gtttttccgg tcgcgaaagc catcctcgct
    49381 cgcgggagct gttaagctcg ctaccgttag ccattgatta tgaagtgctg tttagtgatg
    49441 taccctgcgt ctggttacgc aaagatcatc cggcactgca tgaaacgtgg aatctggaca
    49501 cctttttacg ttatccgcat atcagcattt gctgggaaca gagcgacacc tgggcgctgg
    49561 acaatgtgtt acaggagctg aggcgcgaac gcacgattgc tatgagcctg ccggaatttg
    49621 agcagtcact gtttatggca gcgcaacccg acaatctgct actggcgacc gcgccgcgct
    49681 actgtcagta ctacaatcaa ctccatcaac tgccgttggt tgctcttcct ctcccgtttg
    49741 acgaaagcca gcaaaaaaag ctggaagttc cttttaccct gctgtggcat aaacggaaca
    49801 gccataatcc gaagatcgtc tggttacggg aaaccattaa aaacctttac gcgtcgatgg
    49861 cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata aaatgtaaaa aagttgtaat
    49921 aagcttgtct gaatcgaact tttagccgct ttagtctgtc catcattcca gtaaatgatt
    49981 actcttgtat tcataatgga ccattaagca tggagcgaaa aatggcgact cactttgccc
    50041 gagggatttt aacggaagga catctgattt ctgttcgtct cccctcccag tgtcatcaag
    50101 aagcccgaaa cattccccct catcgtcaaa gccgttttct ggcgtctaga ggtttactcg
    50161 cagaactgat gttcatgctg tatggcattg gcgaattgcc ggaaatcgtc accctgccga
    50221 aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt ttctatttcc tatgccggga
    50281 atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg tctcgatatg gaactacagc
    50341 gtgcgacgcg cgggtttcat agcccacacg cgcccgataa ccacaccttt tccagcaatg
    50401 aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc gcgggcgcag ctcatcacgc
    50461 tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa tgacgatccg cgcgatctgc
    50521 agctgctgcc cattgccgga cgcctgaaat gtgctcatgt aaatcatgta gaagcgttat
    50581 gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac gcccacgatt gaaaagctca
    50641 gtgtctggga gttcgatggc aaacacggct ggaaaagcct gccggatatt cacagccgcg
    50701 ccaacaatcc taccagccgg atgatgcgtt ttgcccagct ctctaccgtg aaggcttttt
    50761 cgccaaattg atagacaacc acaggagtca tcatgtctga aaaattgcag gtggttacgt
    50821 tactggggag cctgcgcaaa ggctcattta atggcatggt tgcacgtacc ctgccgaaaa
    50881 ttgctccggc gagcatggaa gtcaatgcgt taccttccat tgccgacatt cccttgtatg
    50941 acgctgacgt acagcaggaa gaaggttttc cagcaacggt tgaagctctg gcggaacaga
    51001 tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata taactactcg gtaccgggtg
    51061 ggctgaaaaa tgccatcgac tggctttccc gcctgccgga tcaaccgctg gccggtaaac
    51121 cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt cagtatcacc
    51181 tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa tttatgggcg
    51241 gcgtgattca gaacaaagtt gatccgcaaa ccggagaagt gattgatcag ggtacgctgg
    51301 accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt aagatctaaa
    51361 taaaaaaccc gccagcaatc atgcatggcg ggtttttaac gcgctatcga ttttagtgag
    51421 cgtcgataaa gacaatcttc aggataaaca gcagcgcaac gatgattacg cacgggctta
    51481 gatcacgcag acgtccggta ccgattttca tcacgcagta ggagataaag cccagcgcga
    51541 taccttcggt aatcgagaag ctgaacggca tcatcacggc ggtaataaac gccggaacag
    51601 attcagtaag atcctgccag ttcacgcgtg ccagactgga agtcatcagc acgccaacgt
    51661 aaatcagcgc accagctgca gcgtagcctg gcaccatccc cgccagcggc gacagaaaga
    51721 taaccagcaa gaacagcaga ccaacaacca ctgccgtcag accggtacga ccgccaaccg
    51781 atacgccgga agaggactca atataagccg taacggaaga agtaccgata aacgaaccgg
    51841 tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat ttacccttct
    51901 catccgccag gcctgcttta tcggtcacgc caatcagcgt accggaggag tcaaacaggt
    51961 tgaccagcat gaaagagaat atcacccctg ccagcccgag gttaaacgac ccggctaaat
    52021 ctacatggcc cacgacagtc attacgctcg gcggcgcaga aacgatgcca ttgtagtgga
    52081 catcacccag catccagccc agcagcgttg tcaccacgat ggaaaccagc accgccgcgt
    52141 gaatgttgcg cgaggccaga atagcgatga tgaagaagcc gaggatcccc agcagcacgc
    52201 tgtgagaagt caggttaccg atgctgacca gcgtttccgg gttagcgaca atcacacctg
    52261 cattcttcag tcccatcata ccaatgaaca gaccgatacc gctggtgatg cccacacgca
    52321 gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatcgtc agtaacagca
    52381 gaccaatcgc gccccagaag attgcgccca tcccgacttg ccacggcaag cccatagcct
    52441 gtacaacgac aaaagcgaag aatgcattca ggcccatagc gggtgccagt gcaactggca
    52501 ggttagcaaa cagtcccatc ataatactgc cgaatgcagc aatcagacag gtagtaacga
    52561 agacggcgct ggtatccatg ccagcaacgc caagaatttg cgggttaaca aaaacgatgt
    52621 aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc gtcgtgccat
    52681 gttcgcgcag tttaaacacg cgttccagca tcccctggcc agaagcctgg gttgtgtgtt
    52741 gttgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg tataccaaaa
    52801 tgagacaata tggacggctg aagataactt tttttcattg tatttgctta tacggcaacg
    52861 attgcgtcat gcgaattggc atgacgaaaa cgttaaacta acgataaagg aaagacatgt
    52921 cccagataga agcggtattt ttcgactgcg acggtacgct ggtcgacagt gaagtcattt
    52981 gctcccgcgc atatgtaacg atgtttcagg aatttggtat tacgctcgat cctgaagagg
    53041 tattcaaacg tttcaaaggt gtaaaactgt acgaaattat cgatattgtt tcccttgaac
    53101 atggtgttac gttagcgaaa acagaagctg aacacgttta ccgtgcagaa gtcgctcggc
    53161 tgttcgattc agaactggaa gccatcgaag gggctggagc gctcctgtca gcgatcactg
    53221 cgccaatgtg tgtggtatct aacggcccaa ataacaaaat gcagcattct atgggcaagc
    53281 tgaatatgtt gcactacttc ccggataaac tgttcagcgg ctacgatatt cagcgctgga
    53341 agccagaccc ggcgttaatg ttccatgcgg caaaagcgat gaatgtaaat gtagaaaact
    53401 gcattctggt tgatgactca gttgccggtg cgcaatctgg tatcgacgca ggtatggaag
    53461 tgttctactt ctgcgccgac ccgcacaata agccgatcgt tcacccgaaa gtcaccacct
    53521 ttacccatct ttcgcagtta ccggaactgt ggaaagcgcg tggttgggat attacggcat
    53581 agttcttcac actcccttca cttaccccgc ttaaattggc gctcaaaggt aagtaaaggg
    53641 tgtttgatat gtctgtttca cgtcgggtaa tacatcacgg actttatttt gcggttttag
    53701 gaccgttaat tggtgttctg tttcttgtcc tctacatatt cttcgcaaaa gaaccgctgg
    53761 ttcttttggt aataatacag gtacttccat tgtttctctt attgtcgatt actacgggag
    53821 ctattcctgc attgttaacc ggcgtgatgg tcgcctgtct gccggaaaag atcgggtcac
    53881 agaaaaatta tcgttgtctg gctggtggca taggtggcgt cgttatcacc gagatctatt
    53941 gtgcagttat tgtacatatt aagggcatgg cttcctcgga gttgtttgaa aacattcttt
    54001 ctggtgacag tctcgttgtc cgcatcattc ctgcattgct ggcaggtgtg gtgatgagca
    54061 gaatcattac acgtctaccc ggattggata attcatgtcg gaaaacagac tctttaagtt
    54121 aagcgggatt ttttatctat ttgtaattaa ccgttggaga acaggccgga gcgtaattca
    54181 tacatccggc cttatttctt cagcttaacc gagcgctaat tctgcggcag cggctttatc
    54241 agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat cttcggtaac
    54301 cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga caattatcag
    54361 caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa
    54421 cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc cctggatcgg
    54481 gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac aaaaatgacc
    54541 atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc
    54601 atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg
    54661 ggtgaaaaac agattacgca gattggtaat cgttacacct tctccctctt tgccgcgaaa
    54721 gggaatttca tcgaaattat agaaatagca gttatcgtac cagggcttac ctttaaccag
    54781 ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac
    54841 acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc tcatttcctg
    54901 gtaatcttcg gtaatgatta atttcatctt agtgccttat tttatttaaa taatttctgt
    54961 acgaaggctg cataggcagg ccgccagaca tccatttcat ggttcaggcc gggatattcc
    55021 tggtaatcaa acttaatttt tttctgctca agctcagttt tcagcccggc gatatccttg
    55081 ccggttacga catctttatc accaacgacc acagtaaaat tacgtagttg ctggttaata
    55141 gctgccggat cgttcagccg ggccgcgaca ccttcatccg gtacggttgt cgtggtaaca
    55201 ccactgaatg tggccagcca gccaaagctt tccagatgat tcattccgga aaccagcgcc
    55261 tggtacccgc cttgtgaaag ccctgccagc gcgcgaccat cggcatcttt acggacatta
    55321 aaacgcttgc taatcagcgg gataatatcg ttcatcagtt cgcgatctgc cgctttagca
    55381 ttcagcggat aaaagacttt acgtctttcc tgaggcacga aatcttcggg aataatgccc
    55441 ttcgcatcgg tttcggtatc cgggatcacc accagcatcg gtttaatttt cccttcagcc
    55501 agcaggttat ccatgatttg cgggatacgc ccctgatcga tagcggaacg tccggtatca
    55561 ccaaaaccgt gatagaaata gagcactggc aaaggctcgc ccatgccggt gtatcctggc
    55621 ggggtccaga catacatctg acgttcagat tgcaatgcgt tggagtggta agttatggcg
    55681 atcaaatcac catgcgcaac agaacgcgta tccagataac tgcctggcac cagaatcata
    55741 ctggagttaa cctggcgctg aggcttagtc attgcggtgc ctgtatcaat gctgcgtaca
    55801 ccatcaacat tgaaaaaata ctcgtacagg ttgcctttca ggataggtgt gcgccacgac
    55861 cagactcctg cttcgtcttt ggtcatcggg tgaatattgt ccggaaccgg accacccacc
    55921 actacagaga cattttttgc gccaggggca aagtagcgaa aggtcacgct gttatcggca
    55981 ttgacctgag tcacatactg tttcaccggg atagtgggtg ccgggctggc tggcatatcc
    56041 gctgctatgg cccactgagc agaaataccg ctggcaatag ccagcgttaa agcagcaatt
    56101 tttatattca tcgtaatatt cctttcatta agttccatta ccaccagatt tcagcctggg
    56161 caccgacagc aaactggtcg tctttattgt cttcgaaggt gaagccatcc agttcgtttt
    56221 ccagggcttt gatatacgtg gcgtagaaac gaatttccgg acgcgaggtc aacatactgg
    56281 tattgacttt aaaggtatgg aagagcgtgg ttttataacc agactcatta aatttattac
    56341 tattcgcatc tttgttttgc tgggtgaaat agcccagttc aacacctgtc tggttatatt
    56401 ggtcccaaat ataggccggg cgaacaaccg cacgaataga ttcgaaatca gagtgtgcgc
    56461 ctgtttcgta gctataaata tcgttaccga aggagtaaac aatcgcgtta gctacaataa
    56521 aatgatcgcc gatataggct tcgccctgcg aagtcagacg aactgctgtt ccaccagtgt
    56581 gatcaccata ataacgacca ttaaaggtgg taaatggact tgcgccagca taacggccaa
    56641 agttactggc gatagaatta ttcgcgacca ggaaggagaa ttcgttgaag ccacctttat
    56701 caaatttctg cgttaaagat gtgccaaaca tccaggtatc tttccagtca taatacccgt
    56761 tattatcctg attatctttt tccgatgcgc tttcgtttgc cgtgacataa cgaccactta
    56821 ccattaaggt ggctttatcc cataacggga tatctttata gcgtaaatca atggtattgg
    56881 tattaatctg ctgtttgttt tgcaggctgc gatcgtaatc atcgacatct tcgcgaacca
    56941 gcgcgatatc aattttaccc ggaccgactt tccagttttc cagacctaca cccgctgcgg
    57001 catcagtacg ctgcgttttc cagtcaagca tctggatttc aattttcggc gcaccgtgtt
    57061 tacccaccca gaaatcagcc tctggcgcaa agggcaggaa acctttggtg gtaacgtaca
    57121 tatcggagaa ctgcataaag ttctcgccac cggcgttatc gccaaaccag ccggtagagt
    57181 actgctgacc aacgttacca tccatcatca caacggcatc aacccgtttg ccgttttcgt
    57241 tgtagacacg ttgttttaac tgcaaatcaa accagccgga gtattcgtta ccaaagcggc
    57301 ccagagaacc aatcgcccat gatttaggtg aaccatgaga ggcggtcccc caaccagaac
    57361 ggtaatagcc gttgtaacta aagccaattt catctttcac aaacttactg aaatctttca
    57421 gagtcatgga agaataaatc gagcctgtct cactggcatt tttctcttca tttttgacca
    57481 gtacagcatc aggttttgcg gcactactgg tcgggaacgg attggcggca tacccttgat
    57541 cattcgtact tacgctacga ttcaccgtcg ctggcgtata ttttttcttc tcttcatctt
    57601 tatacttttt gagttcgctt tgcgtttctc ttaacgcttt ttctaataac tcaaggcgtt
    57661 gttccaccgt taatgattca gccaatgatt gtgcagaaaa ggctaacggt gccattaata
    57721 agatggcaga ggtaataaga tttcgtctaa acataatatc cctttatggt gcaaagaaag
    57781 aattaacgta tcgcatccag actgttctga atgcgacgat aattaaggtg ctttattggt
    57841 tattttttta atgacagccc ccgcgttttg atcacttctg cataccatcc gaagcttttc
    57901 ttacgcgttc ttgtgaggct tccttcgcca ttatcatcac gatccacata aataaagccg
    57961 tagcgcttag acatttgtga atgagatgca ctgactaaat caattggccc ccaactggtg
    58021 taccccataa tatccacacc atcggcaatc gcttcattta cctgtaccag gtgatcgttt
    58081 aaataggcaa ttcgataatc gtcctgtatc gaaccatccg cttcaacgct gtcttttgcg
    58141 cctaatccgt tctcgacaat aaataacggt ttttgataac gatcccaaag cgtatttaac
    58201 agaacccgta atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga
    58261 ttggggatca tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa
    58321 acacaaccag tcatgtaata actaaaagag atgaaatcta cagtatgttt taaatcttct
    58381 gcgtcacttt cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag
    58441 ccgggatact ggccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc
    58501 atggcctgca acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc
    58561 atattgccga ttttcgcttc ggggagcagg ctatgacagg ctttaactgc ccgcgcactg
    58621 gcaaccagtt gatggtggat agcctgataa acttccgcct cgccactctc ttctgccagc
    58681 cccacgcccg tgaatggctc gtgtaacgac atgttgattt cattaaacgt cagccataac
    58741 gccactttat gttggtaacg agtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg
    58801 accgctcgat tagcccaacc gccgtagttt ttcaccagcc catatggcat ttcgtaatgg
    58861 gataacgtta ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa
    58921 aacgctaacc ccgcttcatt cggttcgact tcgtcgccct gagggaaaat tcgcgcccag
    58981 gcaatggaaa tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg
    59041 taacggtgat aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt
    59101 tccatttttc ccattacgcc atgaggctgt aaatctgagg tcgagatccc tttgccatct
    59161 tcctgccagg caccttccac ctgattggca gctgttgcgc caccccaaag aaatgtttct
    59221 ggaaatgctt tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggttca
    59281 cctgcgctta tctgcgccgt gccgtggggt aatacgtccg taaaatcatc gctattactg
    59341 attaataccg gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa
    59401 atcagccgat cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg
    59461 ccgtccagtt ttacggtgtc gataccgaca tgaatcagga gctccacacc atcatctgac
    59521 tcaatgccaa tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa
    59581 cgcacttcac caaccgaggg cagaatggcg atacctttac ccaacaggcc actggcaaac
    59641 gtggtatcag cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg
    59701 ccctgctcag gtggtgtaat aacctctggt gttttctctt gcggggcacc ctgcgctggc
    59761 tgacgtttag cggtgatgaa atgaagcatc accgtaccga caaatgcgca accgatggca
    59821 atgacaccgc caataacgct ggcccagacg gtgaaatcaa ttcccgttga cgggatggtt
    59881 tgcatgaagg tgaaaatact tggcaaacca aaggagtaca ctttcgtttg cgcgtagcca
    59941 atgatggtgg cccccaaagc cccactgata caggcgataa caaaggggta cttacgcggc
    60001 agattaacgc catataccgc tggttcggtg ataccaaaca ggcccgtcaa cgccgctgat
    60061 cccgccacca cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg
    60121 acctgcgcca taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg
    60181 aagttattga tacacagcgg caccaggccc cagtgcagtc cgaacatgac gaagatttgc
    60241 cagaagccgc ccattaccgc gcctgcaaat gcaggaaccg cctgataaag ccagagataa
    60301 ccggcggcaa tcagttcgct tatccaggtt gatagcggcc ccaccagcag aaaggtgacg
    60361 ggtgtgataa ccatcagaca tagcaatggt gtgaagaaat ttttgattgc cgacggtaac
    60421 cacgcattaa gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata
    60481 accgatgacg agtaattcaa caatgtgacc ggaataccca ggaaatccag ccccagctca
    60541 tccgcttttt gcccgttctc gaaagcagtc agaattaatg gatgcactaa cgctccacca
    60601 atcaccatgg cagtaaatgg attaccgccg aagcgtttcc ccgcggtgta tcccaggatt
    60661 atcgggaaga accaaaacaa ggcatcactg gcgctgaata aaattaaata agtaccactt
    60721 tgttcggtcg tccactgaaa agtgagcgcc agagccagca tacctttcag gatcccggtt
    60781 gccgccatca aaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt
    60841 agcagattac ctttctcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc
    60901 acgctgttga ccgccaggaa gacatcggcc acatggttac ctatgaccac ctgaaactga
    60961 ccaccgcttt ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct
    61021 ttgctttcat cctttaactt aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg
    61081 ttatctgcgc ccccgactcc tgcgactatt tttctggcta actccgtcat aacttgccct
    61141 ctaccgcttt gcggcaaaac tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca
    61201 agcaattttt ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat
    61261 atttcagtgt tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg
    61321 tgaaatttta cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca
    61381 tgcttgcggg taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga
    61441 agcatgttca agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact
    61501 caagctttct tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg
    61561 cattaactgc gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag
    61621 atgcatggca ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat
    61681 aatggttaac gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca
    61741 cagcaacggg ttgggcagca acacgttttg ctgaaagcgt ttaatcgcaa actggcaatg
    61801 gtcagttagc gagatataaa tactgtcctg taattttccc aagcgctcct gcgctaaaga
    61861 gataatacga tcacaggttg ccatcacctc aagaggaata tgacttaaga gttcgcttaa
    61921 tcgcccgttc agttcatgac tgctcaaggc atactctttt tctattccac ttgagttaat
    61981 tctttcgcca gcgcgttttt gaaagccaat tcctcgcccc atgacgactt tttcccgctg
    62041 ttgatcatca ataaccacca caacattatt gttgagaatt ttggtgattt gcatgttcat
    62101 agcaaggacc ttttttataa acaaaaaaac ccgacctcac cagtattctc tggttatgtc
    62161 aggttttgcc tgcgaatgca gtaacaatcc agtcatttat taatggtttt tataacgaac
    62221 atccaggttc ggaaattaat ttaattgcgt gcagagaaaa tagcaatgcg ctattgataa
    62281 aaatatgacc atgctcgcag ttattaactt tgtgt
//
LOCUS       DAZFFI010000012        59965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFFI010000012 DAZFFI010000000
VERSION     DAZFFI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.012"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..118
                     /locus_tag="ACHJ12_001029"
     CDS             <1..118
                     /locus_tag="ACHJ12_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4988855.1"
                     /translation="IFPGQERNLESISGYDKIVVKYNNWITKDNGEFELAVH"
     gene            148..648
                     /locus_tag="ACHJ12_001030"
     CDS             148..648
                     /locus_tag="ACHJ12_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO4988856.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            740..3424
                     /locus_tag="ACHJ12_001031"
     CDS             740..3424
                     /locus_tag="ACHJ12_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO4988857.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3421..4509
                     /locus_tag="ACHJ12_001032"
     CDS             3421..4509
                     /locus_tag="ACHJ12_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO4988858.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4597..5895)
                     /gene="exuT"
                     /locus_tag="ACHJ12_001033"
     CDS             complement(4597..5895)
                     /gene="exuT"
                     /locus_tag="ACHJ12_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4988859.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6378..7790
                     /gene="uxaC"
                     /locus_tag="ACHJ12_001034"
     CDS             6378..7790
                     /gene="uxaC"
                     /locus_tag="ACHJ12_001034"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4988860.1"
                     /translation="MTPFMTEDFLLDTEFACRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7805..9292
                     /gene="uxaA"
                     /locus_tag="ACHJ12_001035"
     CDS             7805..9292
                     /gene="uxaA"
                     /locus_tag="ACHJ12_001035"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4988861.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9375..9926
                     /gene="ygjV"
                     /locus_tag="ACHJ12_001036"
     CDS             9375..9926
                     /gene="ygjV"
                     /locus_tag="ACHJ12_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO4988862.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9931..11175)
                     /gene="sstT"
                     /locus_tag="ACHJ12_001037"
     CDS             complement(9931..11175)
                     /gene="sstT"
                     /locus_tag="ACHJ12_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4988863.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11574..12539)
                     /gene="alx"
                     /locus_tag="ACHJ12_001038"
     CDS             complement(11574..12539)
                     /gene="alx"
                     /locus_tag="ACHJ12_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4988864.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12822..13808)
                     /gene="ygjR"
                     /locus_tag="ACHJ12_001039"
     CDS             complement(12822..13808)
                     /gene="ygjR"
                     /locus_tag="ACHJ12_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4988865.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13887..14570)
                     /gene="ygjQ"
                     /locus_tag="ACHJ12_001040"
     CDS             complement(13887..14570)
                     /gene="ygjQ"
                     /locus_tag="ACHJ12_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO4988866.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(14647..15150)
                     /gene="ygjP"
                     /locus_tag="ACHJ12_001041"
     CDS             complement(14647..15150)
                     /gene="ygjP"
                     /locus_tag="ACHJ12_001041"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4988867.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15235..16371
                     /gene="rlmG"
                     /locus_tag="ACHJ12_001042"
     CDS             15235..16371
                     /gene="rlmG"
                     /locus_tag="ACHJ12_001042"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4988868.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            16562..16645
                     /locus_tag="ACHJ12_001043"
                     /pseudo
     CDS             16562..16645
                     /locus_tag="ACHJ12_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            16642..17058
                     /gene="higA"
                     /locus_tag="ACHJ12_001044"
     CDS             16642..17058
                     /gene="higA"
                     /locus_tag="ACHJ12_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO4988869.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFSAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17103..19121)
                     /gene="fadH"
                     /locus_tag="ACHJ12_001045"
     CDS             complement(17103..19121)
                     /gene="fadH"
                     /locus_tag="ACHJ12_001045"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO4988870.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19447..21798)
                     /gene="ygjK"
                     /locus_tag="ACHJ12_001046"
     CDS             complement(19447..21798)
                     /gene="ygjK"
                     /locus_tag="ACHJ12_001046"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO4988871.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21815..22885)
                     /gene="ygjJ"
                     /locus_tag="ACHJ12_001047"
     CDS             complement(21815..22885)
                     /gene="ygjJ"
                     /locus_tag="ACHJ12_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4988872.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23019..24452)
                     /gene="ygjI"
                     /locus_tag="ACHJ12_001048"
     CDS             complement(23019..24452)
                     /gene="ygjI"
                     /locus_tag="ACHJ12_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4988873.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDSMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24515..24964)
                     /gene="ebgC"
                     /locus_tag="ACHJ12_001049"
     CDS             complement(24515..24964)
                     /gene="ebgC"
                     /locus_tag="ACHJ12_001049"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4988874.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24961..28053)
                     /gene="ebgA"
                     /locus_tag="ACHJ12_001050"
     CDS             complement(24961..28053)
                     /gene="ebgA"
                     /locus_tag="ACHJ12_001050"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4988875.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28237..29220)
                     /gene="ebgR"
                     /locus_tag="ACHJ12_001051"
     CDS             complement(28237..29220)
                     /gene="ebgR"
                     /locus_tag="ACHJ12_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4988876.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29439..29771
                     /gene="ygjH"
                     /locus_tag="ACHJ12_001052"
     CDS             29439..29771
                     /gene="ygjH"
                     /locus_tag="ACHJ12_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4988877.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(29813..31192)
                     /gene="ygjG"
                     /locus_tag="ACHJ12_001053"
     CDS             complement(29813..31192)
                     /gene="ygjG"
                     /locus_tag="ACHJ12_001053"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4988878.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31610..33130
                     /gene="aer"
                     /locus_tag="ACHJ12_001054"
     CDS             31610..33130
                     /gene="aer"
                     /locus_tag="ACHJ12_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4988879.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33284..33907)
                     /gene="nfeR"
                     /locus_tag="ACHJ12_001055"
                     /gene_synonym="yqjI"
     CDS             complement(33284..33907)
                     /gene="nfeR"
                     /locus_tag="ACHJ12_001055"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4988880.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34184..34948
                     /gene="nfeF"
                     /locus_tag="ACHJ12_001056"
                     /gene_synonym="yqjH"
     CDS             34184..34948
                     /gene="nfeF"
                     /locus_tag="ACHJ12_001056"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4988881.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35002..35077)
                     /locus_tag="ACHJ12_001057"
     tRNA            complement(35002..35077)
                     /locus_tag="ACHJ12_001057"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35042..35044),aa:Ile,seq:cat)
     gene            35202..35708
                     /gene="mug"
                     /locus_tag="ACHJ12_001058"
     CDS             35202..35708
                     /gene="mug"
                     /locus_tag="ACHJ12_001058"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4988882.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(35786..37627)
                     /gene="rpoD"
                     /locus_tag="ACHJ12_001059"
     CDS             complement(35786..37627)
                     /gene="rpoD"
                     /locus_tag="ACHJ12_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4988883.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37822..39567)
                     /gene="dnaG"
                     /locus_tag="ACHJ12_001060"
     CDS             complement(37822..39567)
                     /gene="dnaG"
                     /locus_tag="ACHJ12_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4988884.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(39678..39893)
                     /gene="rpsU"
                     /locus_tag="ACHJ12_001061"
     CDS             complement(39678..39893)
                     /gene="rpsU"
                     /locus_tag="ACHJ12_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4988885.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40131..41144
                     /gene="tsaD"
                     /locus_tag="ACHJ12_001062"
     CDS             40131..41144
                     /gene="tsaD"
                     /locus_tag="ACHJ12_001062"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4988886.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41187..42650)
                     /gene="ttdT"
                     /locus_tag="ACHJ12_001063"
     CDS             complement(41187..42650)
                     /gene="ttdT"
                     /locus_tag="ACHJ12_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4988887.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(42698..43303)
                     /gene="ttdB"
                     /locus_tag="ACHJ12_001064"
     CDS             complement(42698..43303)
                     /gene="ttdB"
                     /locus_tag="ACHJ12_001064"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4988888.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43300..44211)
                     /gene="ttdA"
                     /locus_tag="ACHJ12_001065"
     CDS             complement(43300..44211)
                     /gene="ttdA"
                     /locus_tag="ACHJ12_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4988889.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRNAL"
     gene            44418..45350
                     /gene="ttdR"
                     /locus_tag="ACHJ12_001066"
                     /gene_synonym="dan"
     CDS             44418..45350
                     /gene="ttdR"
                     /locus_tag="ACHJ12_001066"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4988890.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45363..45980)
                     /gene="plsY"
                     /locus_tag="ACHJ12_001067"
     CDS             complement(45363..45980)
                     /gene="plsY"
                     /locus_tag="ACHJ12_001067"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4988891.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46085..46453
                     /gene="folB"
                     /locus_tag="ACHJ12_001068"
     CDS             46085..46453
                     /gene="folB"
                     /locus_tag="ACHJ12_001068"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4988892.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            46543..47364
                     /gene="bacA"
                     /locus_tag="ACHJ12_001069"
     CDS             46543..47364
                     /gene="bacA"
                     /locus_tag="ACHJ12_001069"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4988893.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(47527..48765)
                     /gene="cca"
                     /locus_tag="ACHJ12_001070"
     CDS             complement(47527..48765)
                     /gene="cca"
                     /locus_tag="ACHJ12_001070"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4988894.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(48829..49449)
                     /gene="ygiM"
                     /locus_tag="ACHJ12_001071"
     CDS             complement(48829..49449)
                     /gene="ygiM"
                     /locus_tag="ACHJ12_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4988895.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            49691..50992
                     /gene="ygiF"
                     /locus_tag="ACHJ12_001072"
     CDS             49691..50992
                     /gene="ygiF"
                     /locus_tag="ACHJ12_001072"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4988896.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51015..53855
                     /gene="glnE"
                     /locus_tag="ACHJ12_001073"
     CDS             51015..53855
                     /gene="glnE"
                     /locus_tag="ACHJ12_001073"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4988897.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            53903..55336
                     /gene="hldE"
                     /locus_tag="ACHJ12_001074"
     CDS             53903..55336
                     /gene="hldE"
                     /locus_tag="ACHJ12_001074"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4988898.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(55456..55515)
                     /gene="ibsE"
                     /locus_tag="ACHJ12_001075"
     CDS             complement(55456..55515)
                     /gene="ibsE"
                     /locus_tag="ACHJ12_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO4988899.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(55832..55891)
                     /locus_tag="ACHJ12_001076"
     CDS             complement(55832..55891)
                     /locus_tag="ACHJ12_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4988900.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56130..57791)
                     /gene="yqiK"
                     /locus_tag="ACHJ12_001077"
     CDS             complement(56130..57791)
                     /gene="yqiK"
                     /locus_tag="ACHJ12_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO4988901.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLEMESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(57818..58447)
                     /gene="yqiJ"
                     /locus_tag="ACHJ12_001078"
     CDS             complement(57818..58447)
                     /gene="yqiJ"
                     /locus_tag="ACHJ12_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4988902.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            58716..58916
                     /gene="glgS"
                     /locus_tag="ACHJ12_001079"
     CDS             58716..58916
                     /gene="glgS"
                     /locus_tag="ACHJ12_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4988903.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(58959..>59965)
                     /gene="yqiI"
                     /locus_tag="ACHJ12_001080"
     CDS             complement(58959..>59965)
                     /gene="yqiI"
                     /locus_tag="ACHJ12_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4988904.1"
                     /translation="AMDCYAEHEGGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNV
                     FCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIA
                     APECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVL
                     PDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKIN
                     SRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIF
                     HKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN
                     "
BASE COUNT        14421 a        15227 c        15904 g        14413 t
ORIGIN      
        1 catcttccct ggtcaggaaa gaaatcttga aagcatatct ggctacgaca aaatcgttgt
       61 caaatataat aactggatca ccaaagataa tggcgagttt gaactggcgg tccattaatt
      121 atcattgtaa taaacgaagg aatagtaatg aaaaaggtat ttgcaaaatc tcttctggtc
      181 gcagcgatgt tttctgttgc aggctccgca ttggcagtac aaaaggatat tactgtaacg
      241 gccaacgttg atgccgctct ggatatgaca cagaccgata acaccgcgtt gccgaaagca
      301 gtagaaatgc aatatctgcc ggggcagggt ctgcaatctt accagttgat gaccaaaatc
      361 tggtctaacg acgttaccaa agatgtaaaa atgcagttag tttccccggc gcaactggtt
      421 caaagcctgg atgctagcaa gattgttccg ctgacagtaa cctggggcgg tgaagaaatt
      481 aaagctgatg cggcaaccac ttttaccgca accaaaatct ttgcttccga tgcgttaact
      541 aacggttctc tggctaaaaa cctgatgttt gctcaaacca ccaaaggtgt tctggagaca
      601 ggcatctacc gtggtgtagt aagcatttat ctgtctcagg atatctaatc cagataacag
      661 aattaacacg taacaaaggg aggggtagcc ctcccttatt ttaactgttt tagtatttgt
      721 tatggatttc aggattgcca tggataaaaa attactggct cttttgatcc tggcgagtct
      781 cagcccggca gaggcgacat taaccaaaat tcccgcaggg tttgaggtta ttgctcaggg
      841 acagcaggag tatatcgagg tttatttttc agggaaaagt ctcggtaaat attatgcaat
      901 ggttaatctt gataccgtaa catttcttga tccagcaagt ctatataaca agctggaact
      961 ggatgtagac gatcagaaaa tcgcgcatat agtgaaagaa aaattatcgc agccgctagc
     1021 tcgccacggt gaattggctt gcggttatgt acgtactgac tcagggtgtg gttttctgaa
     1081 taccgatacg ctggaaataa tctataatga tgaagaaagt tcggcaacgt tgtttattaa
     1141 tccgcaatgg aattcagctt tcgatgcgaa gtcattatat ttaaatccag acaaaaatac
     1201 tgttaatgct tttatacatc agcaagacat caatgttctg gcacaggatg attaccaatc
     1261 gttgtctatt cagggaaacg gtgcgctggg aataacagaa aatagttata ttggtgcaca
     1321 ctggaatttc aacggttatg atgcagatga tgtcagtgac agtaatgctg acgtcagcga
     1381 tctctattat cgttatgatt ttttacgtcg ttattatgtg caggcgggtc gcatggacaa
     1441 ccgcacacta tttaatgcac aaggcgggaa ctttaccttt aactttctgc cactcggtgc
     1501 aattgatggg atgcgtatcg ggtcgacact gagctattta aaccaggcgc aaagccagca
     1561 aggaacccca gtaatggttc tgctttcgcg caattctcgt gttgacgctt atcgtaatga
     1621 gcaacttttg ggatcgtttt atctcaatag tggttcgcaa tttattgata ccagttcctt
     1681 tccgcctggc agctacagcg tggcattaaa agtctacgaa aataaccaac tcacccgcac
     1741 cgagcttgtg ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc aatggttctt
     1801 acaggcaggt aaaactacat cacaggtttc tgatgatgaa agttcagctt atcagctagg
     1861 agtacgcctg ccattacatc cgcaatatga gctctacgca gggctggcga atgccgatga
     1921 tgtgagtgct ttcgagttag gtaataactg gacggcagat ttaggcgggg tggggaatct
     1981 tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc aaaggtgata tgcaacaggc
     2041 caactggagt aatccgggat ggccgacgtt gggcttttat cggaccaact ctgacggcga
     2101 tgcttgtaca accgacagca gagagagcta taacgcctta agctgttatg aaagtatttc
     2161 cgcgacggtt tcacagaatt ttgtcggctg gaatatgatg ctgggttata cccgcacaca
     2221 aaataacact gatgatagtt tgcgttggga taaacagcag agctttgaaa ataactatct
     2281 tcgccagaca acagcgcaaa gtatctctga aactgtacaa cttagcgctt cccgcgcttt
     2341 tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg
     2401 tggcgataac gacgacaacg gcttgtactt atcgttttcg ttatctgaca cgccaacgat
     2461 ggatagcaat aacaacagcc attcaacaaa tgtgtctacg gattatcgtt atagcgaaca
     2521 ggatggcgat caaacgtcat ggcagttatc gcataccttt tataacgatt cattcagcca
     2581 taaagaactt ggcgtaaccg tcggaggcct gaacaccgat accataaaca gcgcggttaa
     2641 cgggcgttgg gatgggcaat acggaaatgt ctacgctacc gtatctgata gttatgaccg
     2701 taagaatcat gatcatcttt cggcctttac gggcacttat agctctacgc tggctgtcag
     2761 tcgctatggc gttaatttgg gagccagtgg tacagacgat ttgctgggtg cggtactggt
     2821 ggatgtgaaa ggcttctctg aacaggatga agagagtcag gatctgcaac tcgaagcgcg
     2881 ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc
     2941 tggatttcag tctggttttg ttgaggttaa cgacagtagc cagggcaatc agcaggggac
     3001 aacaaacatc attaacggtg cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg
     3061 ctatcgcgaa gtgtctgcca gctttaacta caactatatc ggtcgtttat tactgccggc
     3121 agcggtgaaa aaattcccga ttgtggggct gaacagcgcc atgttactgg tagctgaaga
     3181 tggcggattt acacttgaaa ttaacggcag cgaaaaagag ctctatctgc tttccgggca
     3241 gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc gccagcattc gttacagcgg
     3301 agatgttact tgtagtgtgg tgacttattc acaattaccg gagtcgattc aggttcaggc
     3361 acagttgaaa cagcctaaat tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc
     3421 atgagaaatc gattgattgc ggcgatattg ggcttgtttg gcacgctcac tggcgttcag
     3481 gcagctcctg acgtgaccag tgaaattacg tatgatttgg catctggtag agcggattat
     3541 tacttctgga aggatgaagc gtcggcagga aataatggat atatgtggta tgaatgttcg
     3601 tatcctgacc tccaacaaac ctgtacagct aatggaaata tatcgacagt acaaatctat
     3661 ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc
     3721 attgtaagta gcgatgaagc gccgccagga tgcaaggggg gcaaagggct tcagacgaat
     3781 cttaaggatt ctaatagatc ttcatgtaca gaagatggtc aacattatta tatatacgat
     3841 acaaagtttc ttacgctcta ccttgagcag acagagatga agaatttgcc gattggtggc
     3901 gtctggaagg ggaaagttaa attacattcg aacagcccgg cccaggacta tttcgcaaat
     3961 attaccctga atacgctcga ccccaaccat attgacgtgt tcttcccgga gttcgcccac
     4021 gccacgccaa gggtgcagtt agacttgcat ccaacaggaa gcgttaacgg cagcaactac
     4081 gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg gttttaacgg taatgccatc
     4141 agttatgaaa tcatgctcaa agatgaaggg cgaccagctg cagggcgcag agacggttac
     4201 ttctctatct atcgtcaggg agggaccacc accgacgagg gagaacgtat tgattaccgg
     4261 gtcaaaatgt acaacccgga aaccggtggg caaattgatg tgcgcaataa tgaaaatatg
     4321 gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat
     4381 gccgtgatgt gtgtgccgac acccttgact ctggcagtcg ataaattcag cgtgatggat
     4441 aaacaggccg ggtactacat gggcaaattg tcagtaatct ttacgccttc cttgccaacc
     4501 atcaattaac cctgcaaacc ggacaaacgc ttttttctat ataaacgacg aagccgtctg
     4561 ataaagcggc ttcgtcatta caacttaagt taaacgttaa tgttgcggtg cgggatcgtt
     4621 atgtgtttcc tgcgccacct caatcgccgg tttgttctgc aacacggtcc agataaccag
     4681 cgcacctaac aggtcgaaca ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc
     4741 agccagtgca ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg acatcccggt
     4801 taaaccgttc gccgtcgcca cttcgttacg accgaacacg tcagaagaga gcgtaatcag
     4861 cgcgccagac agtgcctggt gggcaaaacc accgatacac agcagcataa ttgcgacata
     4921 cgggttggtg aacagaccga tcatacccgg gccaatcatc agcacagcac ccagcgttac
     4981 gaccatctta cgggaaacga tcaggttcac accaaaccaa cgctggaaca gcggcggcag
     5041 gtaaccaccg aggatacaac cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc
     5101 gatctctttc aggttaaagc cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa
     5161 agtaccccag gccggttctg ccaggaaacg cggcagcgcg ataccccaga actgacggtt
     5221 acgcaggatc tgaccaacgg acattttctt cgccgtgctc acctggtgct gggcttcctg
     5281 accattaata atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc gtggatgttt
     5341 atagaaaatc agccatgcca tcgcccagat aaagctcaat gcaccggaga tgataaatgc
     5401 catctgccag ctgtgcatta cgattgccca taccaccagc ggcggcgcaa tcatcgcacc
     5461 aatcgaagaa cctacgttaa agtaacctac cgcgatggaa cgctctttcg ccgggaacca
     5521 ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac cgactgcacc
     5581 acgagcaatc gccaggccac cccagctacc agccagcgcg gttgcaccac agaacacggc
     5641 ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca gtacatagcc
     5701 cgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat aggaatactg
     5761 ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca cagtgttacg
     5821 cgtcaggtaa ccaagcacgg tgccaagcgt caccagtgcg atcatatacc aacgtaaccc
     5881 tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag cgcggccatc
     5941 atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa accggcacgc
     6001 cccgaacgtt gccatgaaag tttcgtgcta attcggcttc cgtaccggtt aacccgattc
     6061 atctaacaaa tgaatgaatc ggcaatttgc attccgtcga cttatgattt gcgacggcag
     6121 aaagataact tgtcatacaa ctttaaaagg tgagagccat cacaaatgtg cgaatatttg
     6181 tagggacatt acctgacgac agcgaggcca gtactggcgc ggcctgcaga gagatttacc
     6241 actttgagag taattttttt aactacgttt attgatctaa ctcacgaaaa tatcttcgga
     6301 ctctggaaat tggtgtgata actttgtcag catcgcacca taagcaagct agctcactcg
     6361 ttgagaggaa gacgaaaatg actccgttta tgactgaaga tttcctgtta gataccgaat
     6421 ttgcctgccg tctgtatcac gactacgcaa aagaccagcc gattttcgat taccattgcc
     6481 atttgccgcc gcagcagatt gcggaagact atcgttttaa aaacctgtat gacatctggc
     6541 tgaaaggcga tcactacaaa tggcgcgcta tgcgtaccaa cggtgtggcc gagcgtctgt
     6601 gtaccggtga tgcgtctgac cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca
     6661 ctatcggcaa cccgttatac cactggacgc acctcgaact gcgtcgtccg tttggtatca
     6721 ctggcaaatt gctttctccg tcaactgccg atgaaatctg gaacgaatgt aacgaactgc
     6781 tggcgcagga taacttctcc gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg
     6841 gcaccaccga tgacccgatc gattctctgg agcatcacgc agagatcgcc aaagacggct
     6901 ctttcaccat caaagtgctg ccgagctggc gtccggacaa agccttcaac atcgaacagg
     6961 cgacctttaa cgactacatg gcgaagctgg gcgaagtttc cgataccgac attcgccgct
     7021 ttgctgacct gcaaactgcc ttgaccaaac gtctggatca cttcgccgct cacggctgta
     7081 aagtgtctga ccatgcgctg gacgtggtga tgtttgctga agcgaacgaa gcggaactgg
     7141 acagcattct ggcgcgccgt ctggctggcg aacccctgag cgagcacgaa gtggcacagt
     7201 tcaaaactgc ggtactggtg ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc
     7261 agtaccacat tggcgcgctg cgtaataaca acctgcgtca gttcaaactg ctggggccgg
     7321 atgtaggctt tgactccatc aacgaccgtc cgatggcgga agagttgtct aagctgctga
     7381 gcaagcagaa cgaagaaaac ctgctgccga aaaccattct ctactgcctg aacccgcgcg
     7441 ataacgaagt gctgggcacc atgatcggta acttccaggg cgaaggtatg ccgggcaaaa
     7501 tgcagtttgg ttccggctgg tggtttaacg atcagaaaga cggtatggaa cgtcagatga
     7561 cccaactggc gcagctcggt ctgctgagcc gctttgtcgg tatgctgact gacagccgta
     7621 gcttcctgtc atacacccgt cacgaatact tccgccgcat tctgtgccag atgatcggtc
     7681 gctgggtgga agcgggcgaa gcaccggcgg acatcaacct gctgggcgag atggtgaaaa
     7741 atatttgctt taacaatgcg cgtgactact tcgccattga actgaactaa ggtctggggt
     7801 tgatatgcaa tacatcaaga tccatgcgct ggataacgtc gcggtcgctt tagcagattt
     7861 ggctgaaggc acagaagtca gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc
     7921 tcgtggacat aaatttgcgt taacggatat cgcaaaaggg gccaacgtca tcaaatatgg
     7981 cctgccgatt ggttatgcat tggcggatat tgcggcaggt gttcacgttc acgcccacaa
     8041 tacgcgcacg aatctgagcg atctggatca gtatcgctat caacctgatt ttcaggatct
     8101 gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg
     8161 ggtgcgtaat gagctgtgga tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca
     8221 aatccagaac cgtttcctga aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct
     8281 cttcagccac acctacggct gctcacaact gggcgacgat cacatcaaca cccgcaccat
     8341 gctgcaaaac atggtgcgcc acccgaacgc gggcgcagtg ctggtgattg gtctgggctg
     8401 tgaaaacaac caggttgccg cattccgtga aacgttgggc gatatcgatc ctgaacgcgt
     8461 tcatttcatg atctgccaac agcaggatga tgagatcgaa gccgggatcg agcatttgca
     8521 tcagctgtat aacgtgatgc gcaacgataa acgcgagcca ggcaaactca gcgaactgaa
     8581 gtttggtctg gagtgcggtg gttctgacgg tctttctggt attactgcta acccgatgct
     8641 ggggcgtttc tctgactacg tgattgctaa cggcggtact accgtactga ccgaagtgcc
     8701 ggagatgttt ggcgcagagc agttgctgat ggaccattgc cgcgacgaag caacgtttga
     8761 aaaactggtc accatggtca acgacttcaa acagtacttt attgcccatg accagccgat
     8821 ctatgaaaac ccatcgccgg ggaacaaagc gggcggtatc accacgctgg aagataaatc
     8881 ccttggctgt acccagaaag cgggttccag cgtcgtggtt gacgtgctgc gttacggcga
     8941 gcgtctgaaa acgccggggc tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac
     9001 cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc agtactggtc gtggcacgcc
     9061 atacggcggc tttgtaccga cggtgaaaat cgctaccaac agtgaactgg cggcgaagaa
     9121 aaaacactgg atcgactttg acgcgggtca gctgatccac ggtaaagcga tgccgcagtt
     9181 gctggaagaa tttatcgaca ccatcgttga gtttgccaac ggtaaacaaa cctgcaacga
     9241 gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa
     9301 tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg ccgtttgctt ttccatacct
     9361 gataaggatt tatcatgacc gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc
     9421 tgattggtat cacaacattt ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg
     9481 tctatagcgc cgttattggc gtacattttt ttcttctggg cacctatccc gctggtgcca
     9541 gtgccatcct taatgccatt cgtacattga ttaccttacg cacgcgcagc ttatgggtaa
     9601 tggcgatttt tattgtgctg actggcggaa ttggcctcgc gaagttccat catcctgtcg
     9661 aactattgcc ggttatcggt acgattgtca gtacctgggc gctgttccgc tgtaaagggc
     9721 tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg ggtgattcac aacttctggg
     9781 cggggtcgat aggcggcaca atgattgagg gtagttttct gcttatgaat ggcttgaata
     9841 tcattcgttt ctggcggatg cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc
     9901 ctcccgccgt agacgaaagg ggttaaacaa ttaattacgc agggcgctat ttgccagacg
     9961 atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg tcagttgaag agttcagcgc
    10021 ggtttcgcaa gagtcctgca atacgccgat gataaagccg acggcaacca cctgcatggc
    10081 gatatcgttc gaaataccga acatattaca ggccagcggg atcagcagca gagaaccccc
    10141 cgccacgccg gatgcgccac aggcacacag agaagccact acgctcaaca gcagcgctgt
    10201 gggcagatcg accggaatac ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt
    10261 gattgctgcg cccgccatat tgatggtggc tcccagcgga atagaaacgg aataggtatc
    10321 gcgatccaga ttcagctttt cacacagcgc catattcacc ggaatgttcg ctgcagagct
    10381 gcgggtgaag aaggcataca caccgctttc gcgcaggcac agcagcacca gcgggaacgg
    10441 gttacgacga attttccacc acaccagcaa tgggttaacc accagcgcca ccagtaacat
    10501 acagccaacc agcacgacca gcagttgcgc gtagccccac agtgtggaga aaccggtggt
    10561 tgccagggta gaagaaacca gcccaaaaat accaatcggt gcgaagcgaa tgaccagttt
    10621 caccataaag gtaacggcat tcgacatatc gttgaccagg tttttggtgg tctcgttacc
    10681 gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg atcccgatgt agttaccttt
    10741 cagcagcgcg tcgatggggt tggaaaccat gctcattacc agcccgcgca tcacttctac
    10801 aatgcctgac ggtggcgaaa tatcacccgc gctactggat aagtgcaggg tagaagggaa
    10861 ggcgaagctg aaaacgactg cggccagagc agcagagaag gtacccagca gatagaggaa
    10921 caaaataggg cggatattgg ttttctgccc gtgctggtgg ttagcaatag atgccatcac
    10981 cagcatcaac accaggatgg gggcaacggc tttcagtgcg ccgacgaaca aagtacctaa
    11041 cagaccaaca gcttccgccg cgggttttga gatccatgcc agaagaatcc ccagaacaag
    11101 gccgaccagg atttgtttta ccaggctgcc atgagccaga cgccggaata gccccggtga
    11161 acgttgcgta gtcatttttc gatcctttca ttgtgttgtc gcacatccct ggtgcgttct
    11221 gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac
    11281 agcttcggga agcgaaaaat gctggatttt aaagagaggt catattttgc aacttaatgg
    11341 ttacatttat ttgacataaa aataaagcca acgtgcggct tccagactga tgacaacatc
    11401 aaaaaaggac tcggtcagtc ccctcgcccc gtttggggag agggttaggg tgaggggaac
    11461 aggccagcat cggtgcgaaa aattaccctc accccgaccc tctccctgga agggagaggg
    11521 agaaaacggt gctcatgtta atgacagggt acacggctta ggcagattaa aaattatcca
    11581 ccccgctgct tatcatgccg ataattcacc caggcgttga taataaacgt catcaccaga
    11641 atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc gacaatcagc
    11701 atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat cgagaaacgc
    11761 tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc aaacaggttt
    11821 gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat gctatccacg
    11881 gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg tgtggcgtac
    11941 agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa
    12001 tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg
    12061 agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag gatccagtcg
    12121 aactgtgaaa tcagccagct gcctgtgaag atcatgatgg tacgcagaac aatcgccccg
    12181 agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa atagctgaac
    12241 aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag atagcccgtg
    12301 agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cagtttgcac cagataccac
    12361 cagaaagcgg cgttaaacag taacgacagc gtcacccaga ccagcgacca ggccgcagcc
    12421 tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc gatagccagc
    12481 ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac agtattcata
    12541 gaagttcctt acacataaaa aaacggccaa cgtcggaaga cgtcagccgc tgctttttat
    12601 gcatagacct cgccttccgg caaggtctca cttacaacaa aaaaggaata cgtctgcgta
    12661 ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat gacgatcgac
    12721 cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagaccatt cagccagtca
    12781 atggtctgtt acataccttt tacactgttt tactttggca attatagttt tacgctatct
    12841 gccggaaaaa tcaccccagt ctggcggcgg atctcggtca gcagtttggc ggtgatgcga
    12901 ctgaccgcca gccccggatg atccaccaga tgctcatcca ccagggtagc gaacagctct
    12961 gcttcgtaga gcatggtatt aatatgctgc ggctgggtga gatcctgcat ttggctgcca
    13021 cgcggcacga agcacacttt ctggcattca gacagttttt caatcaccag cgatcctgct
    13081 tcgccctgaa tctcgctcgc caggacagaa tcactgactt ttgagtgctg caaggtgacg
    13141 ctgaaatcac cgtaatccat caccaccacg ccgtgggcat ctacaccgct tgccagcaaa
    13201 ctggcggttg cctgcacgct tttcggctca ccaaataacg ccaccgccga cgccaggcag
    13261 taaaagccga tatccataat tgaaccgtta gagaatgacg gattaaaggt gttgggattt
    13321 tcaccgtcca ggtaacgttg atagcgcgag gaatactggc aatagttgaa aaagacttta
    13381 cgcaatttgc cgactttcgg cagcgcctgg cgcagcaaat gaaagttcgg caggcaggcg
    13441 gttttaaatg cctcaaacag caccacctga ttttcccgcg cacaggcaat ggcggcatcc
    13501 acttccgcca gattcgacgc cagtggtttc tcgcaaatca catgaatttt atggctaagg
    13561 aaaagttgtg tctgggaaaa gtgcagggca ttcgggctgg caatatacac cgcgtcaatg
    13621 gcatcgcttt ccgccatcgc ttccagcgag gtaaacagat gctcgacaga aaaatcattg
    13681 gcgaagtgct gagcctgctc aaggctgcgg gaatatacgg cggttaactt gtatttaccg
    13741 ctctcatggg cggcctcgac gaactggcga gtgatccagt tcgtaccaat cacagcgaaa
    13801 cgtatcataa cgcgtggata ctccataaag ggattctgtc gtcaatgtag cacgcctttt
    13861 tcatcttcga cgtgcgcaga tacgcattat tcttttgccg gacagccatg ctcactaaac
    13921 ggcccaatca tgaccgatgg cccgagatat ttcggctggc gatggaggat tttggcgtca
    13981 atcacggcgc tgacacgagc gagtttctcc cgcagttgag tataaaaacc gtgcttcata
    14041 ttgaggtcag gcgcgttaaa accgatagcg tcgatgctgt actgttttgc cagccagatg
    14101 gcgcgttggt tatgaaattc ttgcgagata atggtgatat ggttttcacc aaaaaccttt
    14161 ttggcacgca ctaccgaatc cagcgttgag aatccggcat agtcgcagaa gatcactttc
    14221 gccggtacgc ctttagcgat caaagccttc tgcatacctg atgcttcatc gtaattttta
    14281 cgtccgttat cgccacttac cagtagccat ttcacttttc cggcgtggta caacgctgct
    14341 gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tggcccccag caataagccc
    14401 acgttacgct ccggaactgc gttgacatcg ctccaggtca gctgcttgct ggcgttgacc
    14461 ataacgcgat cggcaataaa aatggtcgct cctgctataa gcaacaacaa acagccaatc
    14521 ttaagagtcc ggcgagaaaa acaaatacgg agaagaaggc ggggctgcat ggtcgttatc
    14581 cttaacgaaa ggggggaggt cgattttagg cgctaaagaa agaaaagata acgcctgaca
    14641 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    14701 ctcaagctgg tggtactgcg gctccatatg gcagcacaac tggtaaaacg ctttgttatg
    14761 ctctttctcc ttcaggtgcg ccagttcgtg caccacgatc atacgtaaaa acggttccgg
    14821 cgcgttgcga aacacagtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    14881 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    14941 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    15001 atattgccag agggctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    15061 cacatcgccc agacgctgtt cgttaatcag cgttcgcacc tgggaaagta gctgctcggg
    15121 ataaccctga agataagtaa gattgttcat aaccgcccac gaaaaagaga aaaagggtat
    15181 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    15241 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    15301 aacccgctac aggcgtggga agcggcggat gaatatttgc tgcaacagtt ggacgacaca
    15361 gaaatccgcg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    15421 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    15481 aatttacgcc tcaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    15541 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    15601 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    15661 aaagcccgtg acattcacac ttccacgctg gagctgttcg agaaagtgct cggcccgacc
    15721 accactacgc tggcatggaa aaaagcgcgc ctgattaatt gcactttcaa tgaaccgccg
    15781 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccac
    15841 aaccatgcga atgtcttctc ccgcaccggg ctggatattg gcgcacgctt ctttatgcaa
    15901 catctgccag agaatctcga aggtgagatt gtcgatctcg gctgtggtaa cggcgttatt
    15961 ggcctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    16021 atggcggtcg cttccagccg aatgaatgtt gaaaccaaca tgccagaggc gttggatcgc
    16081 tgcgagttta tgatcaacaa cgcgctctcc ggagtggagc ctttccgctt taatgctgtg
    16141 ctctgcaacc cgccgtttca tcagcaacat gcgctgaccg ataacgtcgc ctgggagatg
    16201 ttccaccacg cccgccgctg cctgaaaatc aacggcgaac tgtatatcgt tgccaaccgt
    16261 cacctggatt acttccataa actgaagaag attttcggca actgcaccac catcgccacg
    16321 aataataaat ttgtggtgct gaaaacagtg aagctggggc gtcgtcggta agattgggag
    16381 ttacctgccg gatgcggcgt taacgcctta tccggcctac gttcagcacg ttactcccag
    16441 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gcccagttct ggaatcgcgc
    16501 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgttgttca
    16561 acgtttatgc tacatacgtg aagttatgac gcataaagaa tacgatttct ttaccgctgt
    16621 tcatcgtact aaggggaaaa aatgattgcg attgccgaca tcttgcaagc aggagaaaag
    16681 ctaactgcgg tggcaccttt tctggcgggt attcagaacg aggaacaata cacccaggcg
    16741 ctggaactgg tagatcatct gctgctcaac gatcctgaaa accccttgct ggatctggtg
    16801 tgtgccaaaa taaccgcgtg ggaagaatca gcgcccgaat ttgcggaatt tagtgccatg
    16861 gctcaagcca tgcctggcgg tatagccgtg attcgtaccc ttatggatca atatggttta
    16921 accctttccg atctgccgga aattggcagt aaatctatgg tgtcacgcgt tttgagcggg
    16981 aagaggaaat taacgctgga acacgctaaa aaattggcaa cgcgattcgg catttctccc
    17041 gccttgttta ttgattaacg cgacgtgccg gatgcgttgc atccggcgct tcaaataacg
    17101 atttaaatct ccagcgccag ccgtgttccc tgggcaattg cccgtcgtgc gtccagctcc
    17161 atagccacat cgcagccgcc aattaaatgc acagttttcc cgctatcaat cagcggttgc
    17221 gccagcgcgc ggtttggctc ttgccctgcg cagatcacca cattgtccac tgctaatacc
    17281 tgcgtttcgc cgttgatcac cacatgcagc ccgtcatcgt caatcttctg ataacttacg
    17341 cttgggatca ttttcacacc ccgcgagagc agggtggtgc gatggatcca gccggtggtt
    17401 ttgcctaacc cctgtcccgg tttgctggct ttgcgctgga gcatcacaat ttgccgtggg
    17461 ctacgcggga tctgcattcc ctgcgggctt aagccaccag cctgttgtag gctgctgtcg
    17521 atcccccact cattacagaa cctggcgata ttctggctgg tggattcgcc cggctgactt
    17581 aaatacatcg ccgtatcaaa accaatcccg ccacaaccga tgatggcaac cttgttgcct
    17641 accggcgctt tgtcgcgcag tacatcgaga taactcaata ccttcggatg atcgatcccg
    17701 tcgataggcg gaacgcgcgg cacgatccca ctggcgagga tcgtttcatc gaacgcctgt
    17761 aactgatccg ccgtcacggt gtgattgagt ttcagcgtca cgcccgtcac ttcgatcatc
    17821 cggtggtaat agcgcagcgt ttcgtaaaac tcctctttgc cggggatctg tttggcgata
    17881 ttaaactgcc cgccgatctc gctgtgggca tcaaacaatg ttacctgatg cccacgcgcc
    17941 gccgcgttaa tggcaaacgc cagcccagca ggtcccgcac cgaccaccgc cagatttttt
    18001 ttctgcacgg caggaaggat tggcattttg gtttcgtggc aggcgcgagg attcaccagg
    18061 cacgaggtga ctttgccaac gaagatctga tcgaggcagg cctgattgca gccaatgcag
    18121 gtgttgatct catcggctcg ccccgattgc gcttttgaca gcagttccgc atcagcaaga
    18181 aacggtcgtg ccatcgacac catatcggca tcgccgcgcg agagaatgtc gtcggcaacc
    18241 tgcggatcgt taatccggtt ggtggtcacc agcggcagcg agacgtggcc tttcagtttg
    18301 cgcgtgaccc aagtaaatgc gccgcgcggc acgggcgtgg caatggtcgg aatacgtgct
    18361 tcatgccagc caatgccggt gttgataatg gtcgcgcccg ccgcttcaat ggcctgcgcc
    18421 agttctaccg tttcggcaaa agtgccgccg ccttcgacca ggtcgagcat cgacaaccgg
    18481 tagataataa tgaagtcgtt gccgacgcgt tcgcgcaccg tacgcactac ttctacggca
    18541 aatcgcatcc ggttgcggta atcgccgccc cactggtcac tacgctgatt ggtgcgcagc
    18601 gtcagaaatt cgttgatcaa atacccttcg gaccccatta cctctacacc gtcgtatcct
    18661 gcctcccgtg ccagttgcgc gcagtgggcg aaatcgtcga tcagttgcag gatctcttca
    18721 tgcgtgagtt cgtgaggaac gaaacggttg atgggggcct gcaatgcgga gggagcgacc
    18781 agatgcggtt ggtagctgta gcgcccggta tgcaaaattt gcagggctat tttgccgcct
    18841 tcctgatgta ccgcttcggt aatggtgcga tggtgtggga tctggctggc gtcgttgagc
    18901 attgcgccac cttccatgcc aacgcctgtt aaatctggtg caatgccgcc gctgacaatc
    18961 agcgccacgc cgtgacgggc gcgttcggca taaaacgctg ccagccgctc ggcaccgtcc
    19021 gggtattcct ccagcccggt gtgcattgag cccatcaaca cgcggttttt taacgtggta
    19081 aaacctaaat ccagcggggc gaacagcgac gggtagctca taaaattgtc cagtatgtaa
    19141 aataattgtt atgtggtcgg atgagttcta atttagccgt cgccggagaa aagggaaaag
    19201 gggagtgcgg ggtttgtgat gggattcaaa aagaatacgc ccggtggtaa cgccggatgc
    19261 gacgcttgcc gcgttttatc cggcctgggg gattgcacat gtagggcgga taaggcgttc
    19321 acgccgcatc cgccagtggc acggtgcaga tgccggatgc gacgcttgtc gcgttttatc
    19381 cggccaggag gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccagcagt
    19441 tgcaaattat tgcttacgga aaaaatcgtt atacaacata tacaaatgcg cggcactcca
    19501 ggagaaattt ggtgcgcctt gctgcgcgcc agtcagcggg ttgtaattct cctgaattgg
    19561 gccatcggcg gttaatcctt tggcgtgccg gaagaacgta tccgccagct tcagggcatc
    19621 atcacgataa ccgtaacgct ccatcccttt cagaccaaac cagaactgat ccacccatac
    19681 gcgcccgcgc cagtagatat cagcgccaaa agctggattg gttaacgccg ccgttcccag
    19741 cgggacaaag gtgttgaact ctttaggatc gagcatcacc ttcaccaccg cgtcggcatt
    19801 ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct tccggccctt taccgcgctc
    19861 aacaatcggt ttgcccgcgc agccgttcgc cagcggttta tcttcaatac gcacgtcata
    19921 gaagtactgt gtagccgggt cgaacataca ggtgttgatg tagtccgcga gctgctgtgc
    19981 caactggcga tagcgcttag cttcttccgg tttaccgaga atcgtcgcca tctccgccag
    20041 ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca atagcgagta
    20101 gcccagcaac gttccgtcct gactgcggtt ttcggcgaat ttcaccgtcc agtcgctacg
    20161 tttgccgcca ttagcgacat atttatccaa ctgttctttg tcgataaaac caaagacggc
    20221 ggcatcatca cgccccgatt cccacgatgc agcaacctgt gccggaattt ccagactgtc
    20281 gtactggcct ttctccacca cgcgggcgta gttgttcagc ccagactgcg tctcttcttt
    20341 atcgcctttt ttcaccgtaa acagcatctc gccgctctca gtgttgtggg ctttgtcgcg
    20401 ggtcgcgcca tattccggca cgccgttgcc gttgtgatcg cggttacgta accaccagtc
    20461 gtgataggcc accagtttcg ggtacatctc tgccacccag gttttatctt gggtgacgtt
    20521 atacacttcc atcaccgacc aggcggcaag gctgggtttg gtattacgtt cgttccagtt
    20581 gccgccatcg ccaccacgct cggggctaag gttccacgct atcaggtcgg ggacaaagcc
    20641 cacatcctgc ggacgcacgc ggtcgcctgg ctggatctgc caggagaaga ccgcgcggat
    20701 attctctttg gcgatgtccg gattgaaatg cgccatcgca aacgcctgct tccaggtatc
    20761 ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggtg tgacggtgtt
    20821 aaatttcacc gccccgccag gcgaacgcca gttaccgttg agcgtttcga tcgctttcac
    20881 cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt aaccctttct tcagatactc
    20941 ttcccagcgt tgctgcgagg cggtgagata aaacgccgga cgcgccagaa tatcgcggat
    21001 ctgcatttgc tctttgctaa cttcctgaga ggtcagcaga tgggaatagg tggtatagag
    21061 cgtggtcgaa ccgttgatat gtgccttact ggtaaagcga ttgccgttga tttcagtctg
    21121 caccggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca gatcccaggt
    21181 ggcgcgcact ttgccaaagg tgactttcag gccatcacgg gtggcgctga ttttgcgctg
    21241 gtagtcaggg tattcgccag caatggtttt atcggaaagc ggtttccctt ctttcgcttc
    21301 cagtttttcc agcagttcgc catcccacac cagatccagc ggtttattgc tgatgatttt
    21361 agtttccagt agtgacgtgc gcggcgtggc gaagcgcaga gtcatttcga cttgcacatc
    21421 ttttgctgtc agtttttgca ccagcgcacc gggaatactg tatgcctcca gcgtaaagtc
    21481 gactttcttg ccgtcctgcc agacggtcag gcggtcgaaa ttgctggcca taaagttgat
    21541 gtactcttcc gtcagcagcg caacgcccgg aaagccgccc atggtgttag ggccgtctgg
    21601 caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct ggtgatcgtc
    21661 gtaatcgtaa tccttcatgt actgcggcgc gccagtacgg ttaatcacgt ttttataatt
    21721 gtcggcgtta gcggcatggg cggaaaaact tatcagcaga gcgcaggtta ctggcgttaa
    21781 aatagttttt attttcatga aattacagtc cttattacca gtagaaatat tgcatcagga
    21841 agatttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta tcaaaagccg
    21901 tggcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa gaggtggttt
    21961 catcgtcagc gtggtcaggg cgatgtttgg cgtaggtata agcggtggag agataaacgc
    22021 cctttaacgc ttcatacatg gcgctcacca tcatcccact ttcacttccc ggtacgtcca
    22081 gcccgtcggc gcgtccggta tggtgccagg cgcgagcggc aaagttagga gttagcaggc
    22141 ccgtgagata gagctgtcct gtgccgtcgg tgatttccag accgttcatc caggtgatgc
    22201 cttcggcaag gtcatactgg atgtaaccgt tcatcattgc cggataggtg tatttgtcgt
    22261 catagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga tgtccggcag
    22321 gtgtggcggt gacgctataa cgaaagttgc cgcttaagtt ctgcaccttc agcgcggtca
    22381 taatgtcgcg ggtattggga atgacgtagc ccagatcggg ggtgaagtcg ccccaccatt
    22441 gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg ccatcattgg
    22501 tacgataacc gccatagaaa cggttaattc cgccctcgaa accaccccag ctatggtctg
    22561 gcacccaggc attgccgtcg tggtcggcct gcacggtcca gccttcgccg taaatcaggc
    22621 cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtaccg tcgctgtatt
    22681 tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg ccgtaaaaac
    22741 gaaatgaagg agaagatgtc gaggtaattg caggtgtttt tgtgtcgtca tgttctgccg
    22801 gacgtgcttc ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc
    22861 tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa aagtacagcc
    22921 ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct cctcatccag
    22981 gaagagggaa aatgcctgct cattttttat tattctgctt aatcaacatt gctggctggc
    23041 gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttact gtatttccac
    23101 cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat ggtcagaata
    23161 ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc aatcagcatt
    23221 gaaacaacga taataccggt gcggcgggag cccatacgga aatcgcgtgg caggtgatct
    23281 aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag catggacgct
    23341 gcggcggtca tattaataat agtattcatc agatcctgca cggtattgga accgagcatc
    23401 gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg cgccggaacg
    23461 ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat ttcggagaag
    23521 aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc ggtaaaggac
    23581 accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc cgccatgccg
    23641 tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac gaagacgtta
    23701 atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag gatgatcact
    23761 ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac ggactccgcg
    23821 ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt gaagttcggg
    23881 atcatcgagt caacggtgat ggggtcagca ggctgtacgc cgccaaccag cgccgtacct
    23941 gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac ggaggtaatt
    24001 ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa cagcaccata
    24061 ctgataatgg tggtggcaac cggcgtcata atatattcgt agccgaggaa ggcatacgaa
    24121 gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac gaaccagtag
    24181 gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca cgcgtagaca
    24241 ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca gaagggaata
    24301 aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag ctcaatatta
    24361 ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag caagccgaat
    24421 ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg tgtagggtag
    24481 cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa taaccatctt
    24541 cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac cgatacgctt
    24601 catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct ccgcagcctt
    24661 ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat gtctctttcg
    24721 gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa tagcgtcgat
    24781 gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc aatgagtcac
    24841 cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct tcaacgcagc
    24901 gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta tcgatgatcc
    24961 tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga actcatacga
    25021 cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa agccgtagct
    25081 gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg agttggagcc
    25141 gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct gcagctcgtt
    25201 acagtgctgg gcagcgtgga tgttttcctg ggtatagtgc caggcgctga agttaattgg
    25261 gcgctgcgga accaccagca aaccgttgcc gtggcggtta gtcagtgccg tccagcggac
    25321 atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg catcgacggt
    25381 gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt cgcccggtcc
    25441 acgaccgtaa tacgccacct gatcgtattc gccgttaatc cccatggtga aaccgatgca
    25501 cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt taacctggcc
    25561 atcggcagcg atgcgccaga tgtaggtgca gcgcatcccg aagtcaaaca ccggcggggc
    25621 aataactgtg cggctgatga tcaacacttc accatcgctc tgttctacgg caaagtcgcg
    25681 cagatgttcc tgcatgatct gcaaatgatt cggttgccac agcccttcgt actcctgctt
    25741 gtggttgtcg atcattggct tgaagaagtt gatctttggc tcgcgggtca gcagcgactc
    25801 gccattcacc tgccaggatg tcggtttgcc actcattttt gagaaggtga tcgcgaagtt
    25861 gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac gcgcattatt
    25921 tggtgcgaaa ggcactggtt gcgcggtgtt ttccttcagc gggaactgat aagtggcgat
    25981 agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa tgttgaggaa
    26041 cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc tgttcggcgc
    26101 aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcgacgc gcacctctgc
    26161 gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt tcagctcgcc
    26221 gcgagtcaga tccagcgcgt ggatttttac cggcgcgata acctgtttgt actctttcag
    26281 gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt tatagttgtt
    26341 gggatagtcg ccgtagtcgc cgccgaattt ataccagacg ttgccgttgt catcctgcgc
    26401 ctggatcccg tggtcgcacc actcccaaac ataatgtccc tgaatacaat cgtgcttata
    26461 gaagacgttc tggtactccg tcagcccgcc tggtccgttc cccatcgcat gagcatattc
    26521 acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca gcggcacgcg
    26581 ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt cgtaatgcac
    26641 cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt tacagccata
    26701 gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct gcgcgtgaat
    26761 atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg taatacggct
    26821 gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca tcacaaacaa
    26881 gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag cggtacgcac
    26941 ggagttgata ttgtgctgct tcatcaactg gagatctttc tcgacgcgat ccattccaac
    27001 ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca tcacataacg
    27061 gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa cgcgttgtgg
    27121 caccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat ggtaaagata
    27181 aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc tggcgctggt
    27241 cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt cgccatcaaa
    27301 cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca gcaccacttc
    27361 gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacag tgaaatcgtt
    27421 aatatgcgtt aggtgttttc cgaccagata aacatcgcgg aagatccccg ctgaccacca
    27481 catatcctgg tcttccacgt aggtagagtc cgcccactgc atcacgcgca cacacaacag
    27541 gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc gactgccctt
    27601 gctgaaaccc acatactgac cgttaacata gacttcaaaa taggtttcga cgccgtcaaa
    27661 tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac gttgataggc
    27721 accggttggg ttatcgctgg ggacaaacgg cacatcgatg gggaacggaa aaccttcgtc
    27781 ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg taatatgccc
    27841 ccagtcagcc attaactcag aggtgaaggc ttctggtact tgcagcggat ggtcaaaaaa
    27901 gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc gggcaaaggt
    27961 acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgcggcgcaa gtcggttttc
    28021 gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag agaaatcgga
    28081 accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa tttttactaa
    28141 aaagtagcag gtaaatgcat ttactttaac tggatcacga aaataaacgg cagcactgtc
    28201 cggcaaaaac gattgccgga cagtgtgtaa ggggatttaa cgggtcgtgc cgcgcagttt
    28261 taatttgctg ggaacgaaga ctaacagcgg cagcgcgcga ccatcgcggg ctttttcata
    28321 caccaggtta acgccctgac ttcccatcat ctcggaatgg atgcgcacgg tggagagcgg
    28381 cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa tatcctgtgg
    28441 gatgttcagg cctcgttcat gaattgcccg cagtacgccg atagcaatgg aatcggaagc
    28501 aacaaacagt gccttcggat agtcttcccg cgccagcatt tgttttgcca gttcataacc
    28561 cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg cgtaccactt gtttcagtcg
    28621 gccatattcc gcaaaggcga cctcacgaat atccgccttg ccaggctcat cttcaccgcc
    28681 aataaaacca atacgattaa cgccctggtt gatatagaag tcgatgattt ctttactgat
    28741 gcgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa agtcgataaa
    28801 acagatattg tcggtcaacg cacaggcggc ggcgcgcagg gcgggcgtgg gtttgccgac
    28861 aattaaaata ccggtgacgt ttttaatgtc tggtaatccg ctgtgttcat aacagttggt
    28921 gagctcaatg cccagctttt cgcactgagt ttcaatgccg tggcggatcg ccagatagta
    28981 aggatcgttg atctccagct cctgttggta gctgtagata gccagaatat ggtgttggtt
    29041 gactgcacct gtctggagtt tacgggcact actggtcttg tactccagct tttcggcgat
    29101 ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt catttaagac
    29161 cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta gtgttgccat
    29221 gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg cttatcgggt
    29281 cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac acagcggctc
    29341 acgaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga aaaacttttg
    29401 ctatagttta gtaaaatttt acccgaaggg aggaaacaat ggaaaccgtg gcttacgctg
    29461 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    29521 ccgacaagct gtacatcgta caggttgatg tgggggaaaa aacactgcaa accgtgacca
    29581 gcctggtgcc gtactacagc gaagaagagc tgatggggaa aacggtggtg gtcttatgca
    29641 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    29701 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    29761 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    29821 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    29881 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    29941 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    30001 tatcaacaaa ctcaattgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    30061 gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt tgctcagcct
    30121 gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc gcacaggcca
    30181 gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga actgaaaaca
    30241 cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagc gctttggcaa
    30301 ggcagaggat atccggctgt acgttctcat gctcgcaggc gaacatcttg cccgtgcgcc
    30361 ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg catagcttac
    30421 gtacggcggt gagatagccc ggcggcggca gaattacgcc accttcaccc tgaatcggtt
    30481 cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcgttaaga gccgtgcgca
    30541 tggcttcgat attgccaaac ggcacatgac ggaagcccgg cagcagtggc ataaacggtt
    30601 tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg tggaacgcgc
    30661 cgctggtggc aataaaagta aacttgccgc gcggtgactg gtaagctttc gccagcttca
    30721 gcgctgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc agtttaccgg
    30781 gcgttagcgc agcaagggtt ttcgccaaca tcgcccgtaa cggatcgagc agctcctggc
    30841 tgtgcagcgg ttgtttcgca agttgattct gtacggcgga aaccacaact ggattacggt
    30901 gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt ccctgggtgt
    30961 cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc ccgccggcgg
    31021 taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag tattcaatca
    31081 cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca atgagattca
    31141 gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc aaaatatgct
    31201 ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc gccactccgg
    31261 ctgcgatcgc gaaaatcggg ataaacacaa attcggttat catttgtgca atatttaatg
    31321 ctatctgtta acatttgttg ttattttgca cggtttttgt cgggtatgga tttgatgatt
    31381 gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa gataattaac
    31441 gttttcgtat agtaacagag tgaaagtatt ctaaatcaaa gaattagatt tgtgctttaa
    31501 tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg gggccgacat
    31561 aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca tgtcttctca
    31621 tccgtatgtc acccagcaaa ataccccgct ggcggacgat accactctga tgtccactac
    31681 cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga gcggctttac
    31741 cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata tgccaaaagc
    31801 ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg gcatcgtgaa
    31861 aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac cgatggtgcg
    31921 cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg aagagattgc
    31981 ggcggtggag ccgctgtaca aagcgctgaa cgccggacgt accagtaagc gtattcataa
    32041 aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc ttcgctggcg
    32101 ggcgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt ggtttgttgc
    32161 tgccccggtg gtgacgtatt tcctctgtgt gttagtggta ttgttggcaa gcgcttgttt
    32221 tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt caggcactga aggtggcgac
    32281 cggagagcgt aatagtgttg agcacctgaa tcgcagcgat gagctggggc tgacattacg
    32341 cgcggtaggg cagcttggcc tgatgtgccg ttggctaatt aacgatgtct caagccaggt
    32401 gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac tgaacgaaca
    32461 tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc aaatggcggc
    32521 gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta tcaccgccag
    32581 taatgcggca gtgcagggtg gggaggcgat gaccacggtg atcaagacaa tggacgatat
    32641 cgccgacagt acccagcgca ttggcaccat tacttcgctg attaacgata ttgcgtttca
    32701 gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg aacagggcaa
    32761 aggttttgca gtggtggcag gggaagtgcg tcatttagcc agccgcagcg ctaatgctgc
    32821 caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg gttcgcagca
    32881 ggtacacgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa acgtcaccca
    32941 gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt ccagcctgac
    33001 ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg tggaagagag
    33061 tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg cggtgacggt
    33121 gctgcattaa tcggtatgcc ggatccggcg gttggagcac aatgcctgat gcgatgctgg
    33181 cgcatcttat caggcctgcg gggtgtgtag cgggtgtagg ccggataagg cgcgtcaagc
    33241 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgattaatcc agttgcgtaa
    33301 tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca tcactgatat
    33361 cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc cgcttcattt
    33421 gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct tcaatcatct
    33481 ccacctgttc gcggttttct tccagccact gcgcgccttg ttcggtcagc gcaatctgct
    33541 tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga aaatccagcg
    33601 ttgggtagat gacgcccggg cttggggtgt aattcccctg ggttaggttc tcaatcgctt
    33661 taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca
    33721 attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg cgaccaccgc
    33781 agcattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct gacttctcac
    33841 ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat
    33901 ggctcatatc agcctccttt tgcttgatta gatatatcta atttatatct gatgtgagca
    33961 ttactgcaag tacaaaaatg atttttaaac gtggaagcac ctcacatcca ggccggataa
    34021 gacgttcacg ccgcatccgg cagcattgct atggcactga atgccttcac atacttcaga
    34081 tatatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttacgaa
    34141 aattatttag atatatctga ttactatcac gaaggcgata acaatgaata actccccccg
    34201 ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa ctgactgtgt tacgcgttga
    34261 gcgcatcagc gccggttttc agcgcattgt cctcggcggc gaggcgctgg acggttttac
    34321 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    34381 gccgccaacg gtaacggaag agggcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    34441 tgactatacg ccgctgtatg acgaactacg ccatgaactg gcgatcgatt tctttattca
    34501 cgacggtggg gtcgccagcg gctgggcgat gcaggcgcaa ccgggcgata aacttacggt
    34561 ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg
    34621 cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca aacttgccgt
    34681 taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg attatctcgc
    34741 gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg
    34801 tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg gcgaaggtaa
    34861 agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac agcgggtacg
    34921 tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg ctgacgattt
    34981 ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat
    35041 tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac
    35101 tccgtgtcgc aatccagcca ttaccgtgcg cctgctgttt ttataaacaa tgtattttca
    35161 atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg
    35221 ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc gccgggactg
    35281 gttttccctt tgctcatcca gcaaatcgct tctggaaggt gatatatcag gccgggttta
    35341 ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcgtca
    35401 ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg
    35461 caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg ttggcgattc
    35521 tgggcaaaca agcatatgaa cagggattca gccagcgcgg tgcacagtgg gggaaacaaa
    35581 cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg
    35641 tttcactaga gaaactggtt gaagcatatc gcgagctgga ccaggcgctg gtagtgcgtg
    35701 ggcgataaaa acgccaccga aaaggtggcg tttgtgcaga gggactagtg ccgggtgcgg
    35761 cgtaacgcct gatccggcct accgattaat cgtccaggaa gctacgcaac acttcagaac
    35821 ggctcgggtg acgcagtttg cgcagcgcct tcgcttcgat ctgacggata cgttcgcggg
    35881 taacgtcgaa ctgtttaccc acttcttcca gcgtgtggtc ggtgttcata tcgataccga
    35941 aacgcatacg cagaactttt gcttcacgcg cggtcaggcc agccagcacg tcgtgcgttg
    36001 ccgcacgcag gctttcggtg gtcgcagaat ccagcggcag ctcgagggtg gtatcctcga
    36061 tgaaatcccc cagatgcgaa tcttcatcat caccgatcgg cgtttccatg gagattggct
    36121 ctttggcgat cttcagcact ttgcggatct tgtcttccgg catcagcata cgttcagcca
    36181 gttcttccgg cgtcggttca cggcccatct cttgcagcat ctggcgagaa atacggttga
    36241 gcttgttgat ggtctcaatc atatgcaccg gaatacggat ggtgcgcgcc tgatccgcga
    36301 tagagcgggt gatcgcctga cggatccacc aggttgcgta ggtggagaac ttgtaaccac
    36361 ggcggtattc gaatttatca accgctttca tcagaccgat gttgccttcc tgaatcaggt
    36421 caaggaactg caagccacgg ttggtgtatt tcttggcgat agaaataacc agacgtaagt
    36481 tcgcttcaac catctctttc ttcgcacggc gggctttcgc ttcaccgatg gacatacgac
    36541 ggttgatatc tttaacctgc tcgatggtca ggccggtttc ttcttcaatc tgctgcagtt
    36601 tctgcagggc gcgatgcact tcttcagaga catcgtgcag tttttccgac cacggcttgt
    36661 tcatcgcaat tgccgcgttg aaccaggtat cgctggtttc gttgccggta aacagggtaa
    36721 tgaagttttt cttcggcatt ttgcactgct caacgcagag cttcatgatc agacgttctt
    36781 gcgtacgaac gcggtccatc atgacgcgca tgctgttgac caggtagtca aactgcttcg
    36841 gcaccaggcg gaactgtttg aatacttcag acagtttcag gatctcttcc tgagcggcag
    36901 cgtgactgcg acctttcgct ttgatggtgt cacgcgttac aacgtactga gcgcgtagtt
    36961 ccgcaaattt ttcgcgagcc agttccgggt cgatgctgtt gtcatcatcg gcgctgtcgt
    37021 catcgccatc ttcttcgtct tcatcttcgt catcgtccag atcttcctgg gaaagctcag
    37081 aaccgacgtg agtggcggta ggtgccagat cttcttctgc gttcgggtca acaaagccgg
    37141 tgatcagatc ggacagacgc gcttcttctg cttcaacacg atcgtactgt tccagcagat
    37201 aggtgatcgc ttccggatat tcagcaacgg agcattgaac ctggttgatc ccgtcttcaa
    37261 tacgcttagc gatgtcaatt tcgccttcgc gggtcaacag ttcaacggtg cccatttcac
    37321 gcatgtacat gcgtaccggg tcagtcgtgc gcccgatttc agattccacg ctggaaagca
    37381 cctgcgcggc ggcttcggca gcatcttcgt ccgcggtgtt ttcagccagc atcagatcat
    37441 cggcatccgg tgcttcttcc atcacctgaa tgcccatgtc gttgatcatt tggatgatgt
    37501 cttcgatctg atctgaatcg acgatatctt ccggcagatg gtcattgacc tcggcatagg
    37561 tcagatagcc ttgctcctta ccacgggtga caagaagttt cagctgtgac tgcgggtttt
    37621 gctccataag acggtatcca cacttatttc atgaggttgg tgttgggcga ttgacccgat
    37681 gtccttcagc cgttaacgct gaaatccaac ggttgtttac cgccaacgat aattacgagg
    37741 gcgtacttat atatttgccg ctgcctctca gtgcggctgt cgggggcttc ccgatcgctc
    37801 ttcggcactt aagccgttaa atcacttttt cgccagttcc tggtttaatg tccagagctc
    37861 caggcgttct tcgttgctta aaccatgcgt gcgctcacga gcgattaact cttcctggcg
    37921 cagttcaagc agcgaatcaa acatatggtt gagtgagtcg gtgaaggttt gctcagcaat
    37981 attcttatct gctatatcgt cccacatcga cagtttttca agggtggcag cattatttgt
    38041 accacgatag tgctctaaaa gttgcccggt ggtcagacct ggctgggaga gacaagtgtt
    38101 gaccagttct ctgaataagc caagtccagg gagcttattt tcatccagat tctcaagcgg
    38161 cgggaccaac gtcgctaatt ctggattttg caccagcaac cctataagta tacgcatggt
    38221 cgtgcgtttt agctgcggaa cagggcgaga aacgccgctc tctgccgctt ttggcattaa
    38281 tcgttcaagc tggctgtcat caagtatgcc taatttgttg cctaattcct gacgaagata
    38341 tattcgcagc gtttcgcccg gcacttgcga tatcaatggc agtgccagcg tactcaaacg
    38401 tgcgcgcccg tcaggggtac tcagatcaac ttgcggcatc aggctgttaa acagaaatgc
    38461 ggagagtggc atcgcctgct ccatccgcgc ttcaaacgct tctttacctt cttttcgtac
    38521 cagcgtgtca gggtcttcgc catccggcaa aaacataaag cgtagctgac ggccgtctgt
    38581 catgtaaggc agcgccgttt ccagcgctcg ccatgcggca tcgcggcctg cacggtcgcc
    38641 gtcataacag caaatgacat tgttggtcgc gcggaacaac agttgtatgt gatcggcggt
    38701 ggttgacgta cctaacgacg caacggcgta attaatgccg tattgcgcca gcgccaccac
    38761 gtccatatag ccttcgacca caagcagacg attgggttca gcgttatcct gctgcgcttc
    38821 ataaagaccg taaagctggc ggcctttatg gaaaatgtct gtttccggcg agttcaggta
    38881 tttgggggta tcgttgccca gcacgcgccc gccaaaacca atcacccgac cgcgtttatc
    38941 gcgaatgggg aacatcaccc gctcgcggaa acgatcgtaa ctgcgtccct gatcgttagt
    39001 gaccaacatc cccgcatcaa tcaatgactg gcgattttct ggattgccgc caaaccgctt
    39061 caggacgttg tcccagccgg ggggcgcaaa accaatcgca aagcgagcga taacctcgtg
    39121 gcttaatccg cgtttttcca gatactggcg cgcagacgtg gcaacaggtt gttgtaaaga
    39181 ttgttggtaa aacgtattca gaccgtccat caactgataa agcgtttgcc tctgatggcg
    39241 ctcgatctgg ctggggccgc tgcctgcttc aaatggcact tcaagattgt gcattgctgc
    39301 cagctcttcg accgtttcaa cgaactcgag cttgtcgtag ttcatcagga agtcgatcgc
    39361 gttgccgtgc gcgccacatc caaagcagtg gtaaaactgt ttctcaccgt taacggtgaa
    39421 tgacggggtt ttctcgttgt ggaatggaca acacgcgtgg aaattcttgc cctgcttttt
    39481 cagcttcaca cgggcatcga tcagatcgac gatgtcagtg cgtgccagca gatcattaat
    39541 gaatacgcgt gggattcgtc cagccatagg ccccgatttt tagcaattca taaacgaaaa
    39601 taagccgcgc attcctttcg gaagcacggc cttacaacta caactcggtc tgatcggaga
    39661 gcaacgctct cggggaatta gtacagacga gtgcggcgtg cgttttcgcg agccagtttc
    39721 ttcgcgtgac gtttcactgc agaagcttta gcgcgcttac gttcggtagt cggtttttca
    39781 tagaactcac gacgacgaac ttccgccaga acacctgctt tttcgcagga acgcttgaag
    39841 cgacgcagag ctacgtcgaa cggctcgttt tcacgtactt taattaccgg catgtgcctc
    39901 tcacctttga ttaattcggt ttgccgctgg catcaacgcc agcttatttc aaaatggtgc
    39961 ggaattttac tgcaattgct gctgctttgt aaagcaccgc ggcctttttt gaaagggact
    40021 tttataaggg tgaggagtat acacgaggct ttctccaggg gcgaacaaag ttttacatca
    40081 acccgcattg gtcctacact gcgcggtaat aaagcgaggt aaaacaagtc atgcgtgtac
    40141 tgggtattga aacttcctgc gatgaaaccg gcatcgccat ttacgacgat gaaaaaggtt
    40201 tgttagccaa ccaattgtat agtcaggtga aattgcacgc tgactacggc ggcgtcgtgc
    40261 ctgaactggc ctcccgcgat cacgtgcgca aaaccgtacc gttgatccag gcggcgctaa
    40321 aggagtctgg tttaacggca aaagacattg atgctgtggc ctataccgca ggccctggat
    40381 tagtcggcgc actgctggtt ggcgcaaccg tggggcgttc tctggcgttt gcctggaacg
    40441 ttccggcaat cccggtacac catatggaag ggcatctgtt agcgccgatg ctggaagata
    40501 acccgccgga atttccgttt gtcgcgctgc tggtgtccgg cggtcatacg cagttaatca
    40561 gcgtgactgg cattggtcag tacgagctgc tcggcgagtc tatcgatgat gccgccggtg
    40621 aagcgtttga taaaaccgcg aagctgctgg ggctggatta tcctggcgga ccgttactgt
    40681 cgaaaatggc ggctcagggt actgccgggc gctttgtttt cccgcgtccg atgaccgacc
    40741 gtccggggct ggatttcagc ttttctggtc tgaaaacctt tgcggcgaac acgattcgtg
    40801 acaacggcac cgacgaccag acgcgtgctg atatcgcccg cgcctttgaa gatgcggtgg
    40861 tcgatacgtt gatgattaag tgtaagcgag cgttggatca gacgggcttt aagcgactgg
    40921 tcatggcggg cggcgtgagt gctaaccgca cgttacgggc gaagctggcg gaaatgatga
    40981 aaaaacgccg cggcgaagtg ttctacgcgc gtccggaatt ttgtactgat aacggcgcga
    41041 tgatcgccta tgccggaatg gtgcggttta aagcaggcgc gacggcggat ctcggcgtta
    41101 gcgtgcgtcc gcgctggcca ctggcagagt taccggctgc gtaaaactat ccggtgccgg
    41161 agagcaattt ccggcaccgt cctcacttaa agcaacacca cgggcatcca cagcaagccg
    41221 gtaatcacca gcaacacgag gaatatcagt ccaaaaatcg cccccagtcg ccagtaatcc
    41281 accgttggca gataaccact accgtagtaa atcggactgg gtccggtggc gtatggtgta
    41341 agaatgctcc ccagaccaat tgccgcgcca accatcaggc agaacaccgg cagcgggatt
    41401 tccggcatcg ccagcgcagc ggcaatcatc atcggtgcga gggcggaggt atacgccgtg
    41461 gcgctggcga aaaagtagcg cagcagataa aacaccacaa tcaacgccac catcaccatc
    41521 gttggcgaat aaccgcttaa gctgcctgct aacagtttgc caaaccagct aataaaacca
    41581 gtgttgttga gtccggtagc gagggtgata agcgaggcca gccagaagaa aacgttccac
    41641 gccgctttat tactgacaat gtcgtcccaa ctgataatgc gcagaagcag catcagtgcc
    41701 accacgctgt aaccgaccat cgcggcatcg atataatcac cgccgaaaat ccacagcacc
    41761 agcgcgccta ccatcagccc cagcatccgt ttttcacgcg aacagagcgg gcccattgcc
    41821 tgcagttccg tctctgccca gcgcggcacc tgatcgccag acttcagtac cggcgggtac
    41881 agcacataag ccagccaggg aaccagcagc accagcaaaa tactgagcgg caacatcccg
    41941 aggaaccagt cgccccaact cagcgtggcg tgagatgcgc ttttcatcag tccaattaac
    42001 agcaagtttg gtgccatcgc cgtcaagaaa atggcgctgg tcacgcagtc ggcaacaatc
    42061 cccatccaca tgatgtacga gccaatgctg cgcgaactgc tgtcgtttgg ttgtgattga
    42121 tagagcggtg gcaggttacg gatgatggga tagataatcc ccgcaccacg cgccgagttg
    42181 gacggtgtta caggtgcgag gatcagctcg gagaacatca ccgcatagcc gagaaacagc
    42241 gtgcgatgtc ccatcttttt caccagaaac agcgcaatgc ggcgtccaag cccggttttt
    42301 tcatagcctg tgccaaacat aaaggcggcg aaaatcagcc agataaccga attagaaaaa
    42361 ccggaaaccg cccacgagag ggattttgca gtgaatttaa agcctggctg ggcgagctgt
    42421 tcggggctga acagcagcca gggagagagt atggcgatga tggagatacc caccatcgcc
    42481 accacggcac ccggcacggg ttcgaggatc agtccaacga tcacgccagt aaaaacggca
    42541 aagtagagcc aggtatggtt ctccagccct gcgggaaccg gaagtagagc aataatggcg
    42601 atgaccgcca gcggcgcaag atatcgccac cattcagtgg aaggtttcat cgtgttatgc
    42661 ctgcgaaaaa ggagaggggt gagcctctcc gggagggtta tttgatgtaa tggacgtgct
    42721 cgcagatctc ttccacgatg ggatcgcggc gttcggcgaa cagctttttg ttttcggcta
    42781 tcaggttgtt gccgtgggta tcaatagaga caatcagcgg gccgaactct ttgacccggc
    42841 agacccacag tgactccggc atcccgagtt ctgtccagtg cacttcttca atctcttcta
    42901 cctgggttgc cgccagcacc gcgcagcctg ccgggaaaat cacatgtagc gccttgaatt
    42961 tctggcagcc ttcttcggtc agcggcccca taccaccttt gccaaccacc agtttcacgc
    43021 cggtctgctc gataaattca cgttcaaaac tttccatacg catactggtt gtcgggccaa
    43081 cggaaaccat ctcccattta tcgccgtttt tgcgcacgat ggggccagcg tggaaaatcg
    43141 ctttgccgtt gagatcgtaa gggatcggac gtttcagttc gatcaagcgg cggtgacaaa
    43201 cgtcacggca ggtcaccagc gtaccggtca ggtagatcac atcgccgacg cgaatatctt
    43261 gcagatcttc agctttgatc ggggttgtca ggatcttttt cataacgcgt tccgggtgtg
    43321 agacagattt tcaaaggtga gatcggcatg aaccagcagt gtgccgcgac gatgcgccca
    43381 gcagcctgta gaaacagcaa cgccgatggt tgacggatgg cgggcggcag attcgatatg
    43441 cacgcccatc actgaactgt tgccagtcag cccttgtggg ccaattccca gacggttgag
    43501 tccttcttcc aggcgtagct ccagctctgc cgcttttgga ttgggatggc ggctgccaat
    43561 cgggcgcaaa atggcttttc gcgagagcac ggcggcggtt tccaccgagg tggcgatgcc
    43621 cacgcccacc agtaccggtg gacaggcgtt tacggcgagg gtggagatat tttcgaagac
    43681 gaatttcact acgccttcgt agccttctga cggcattaac actttcgagc ggccaggtag
    43741 cgtgcagccg ccgcctgcca tgtaaacttc gatttccgca tcgtcattgt cggggatgat
    43801 gtcccaggtg acccacggta cgccgctacc ggtatttttg ccggtgttta cttcgtcaaa
    43861 aatttctacc gcattgtgac gtagtggcgc tttgacggtt gcctcttcca cggcttgttt
    43921 gagtatgctt tgcagctcgc caagcagtgg gaagcgggaa ccgactttaa cgaagaacat
    43981 aatctccccg gtgtcctgac aggcaggacg attcaggtca atcgcttttt gcatgttgtc
    44041 gaacatcgta tggtagataa ttttccccat cgacgacgtt tcggcatcct ttagctgttt
    44101 tagtttatcc accacgtcat caggcattcg ggtagaaatc atggcggtaa agttagcgac
    44161 aatctctgtc aacttattca ctgcctgttg cttattactt tcgctcatca tggcaactcc
    44221 agtttttgta tttgtctgga aaaattcacc gccacaaact accactaaaa atgtggtttt
    44281 atattttttg ccaccgtgaa tatgtaatta acagttcgtg aatcaacgcg aaaataagaa
    44341 tgaatattgc gccagagcag ggaatggcaa tgttttgcga aatacactgc gcttatcaca
    44401 actgcggata attaacgatg ctgaatagct ggcctttagc caaagacttg caggtactgg
    44461 tagaaatcgt ccacagcggg agttttagcg ccgcagcggc gacgcttggg cagacgcctg
    44521 catttgtcac caagcggatc caaatccttg aaaatacgct ggcaacaacg ctgctgaacc
    44581 gctccgctcg cggcgtggcg ctgactgaaa gtggccaacg ttgttacgaa catgcgctgg
    44641 aaatcctcac ccaatatcag cgactggtcg atgacgtcac gcaaatcaaa acgcgcccgg
    44701 aggggatgat tcgtattggc tgtagttttg gttttgggcg cagccatatt gcgccagcca
    44761 ttaccgaact tatgcgcaat tatcctgagt tacaggtgca ttttgaattg ttcgatcggc
    44821 aaattgattt agtgcaggat aatattgatc tggatattcg tattaatgac gaaattcctg
    44881 attattatat tgcgcatctg ttaacgaaaa ataaaagaat attatgcgca gcacctgagt
    44941 atctgcaaaa atatccgcag ccacaatcat tacaggaatt aagtcgtcat gactgtctgg
    45001 tgaccaaaga gcgcgatatg acccacggaa tatgggagtt ggggaacggt caggagaaaa
    45061 aatcggtgaa ggttagcggg catctttcct ccaatagcgg cgagatagtg ttgcaatggg
    45121 cgctggaggg caaagggatt atgctgcgtt ccgagtggga tgtgttgccg tttctggaga
    45181 gtggcaaact ggtgcaagta ttgccggagt atgcacaaag cgccaatatc tgggctgttt
    45241 accgggagcc gctctatcgc agcatgaaat tacgtgtctg cgtggaattt ctggcggcat
    45301 ggtgccagca acggctgggc aagcccgatg aaggctatca ggtcatgtag atccaccaga
    45361 aatcactcgg gatccttttc gcgctttctt ttgaatttcg tccagatttt tgtctcctga
    45421 cgacgccaca gacgctggat gttgtcatga tgacgcagca ggatcaggca agagagcatc
    45481 gaaaccggga aggtgaattg tggcttaaac caccatacat aaaacggagc aatcagtgca
    45541 ctgacaatcg ctcccagcga cgagtatccg ctcaatagca cggtcagtaa ccaggttccc
    45601 gccattactc cggtgagatc ccagccaatg ggtgcgatgg caccaaaagc ggtagcaacg
    45661 ccttttcctc ctttaaatcc gaagaaaacg ggccagatgt gtccaagaca ggcggcgatg
    45721 gcaattaagc ctagccagaa ggggctgaca cctaattcat acgcgcccca gacgggcaac
    45781 attcctttca gaacgtcgaa aatcagtact gctacggctg ctcccttgcc accgatacgt
    45841 aacacattgg ttgcgcctgg attgccggag ccgctggttc gcggatcggg cagcccacac
    45901 aagcggcaaa ccagaatggc actggaaatg gagccgcaga ggtacgcgat gaggatcatt
    45961 ccaggcgcga ttgcactcat aagctgttcc gttttgaaaa ttcgtgttca acgatgaatc
    46021 tgtggataat acgcacattt cgccggaagt ggtatccggt tagccaaaaa gcaggcagga
    46081 cgtgatggat attgtattta tagagcaact ttcggtaatc accactattg gtgtttacga
    46141 ctgggaacag accatcgaac agaagttagt gttcgatatc gaaatggcat gggataaccg
    46201 taaagcggcg aaaagtgatg atgtggcgga ttgcctcagt tacgctgaca ttgcagaaac
    46261 ggtggtcagc cacgtcgagg gggcgcgttt tgcgctggtg gaacgcgtgg ctgaagaggt
    46321 ggcggagctg ctgttagcac gcttcaactc gccgtgggtg cgtatcaaac tcagcaagcc
    46381 aggcgcagtg gcgcgggcgg cgaatgttgg cgtaatcatt gagcgtggca ataatctgaa
    46441 agaaaataat taattttaca gctgttaaac caaacggtta taacctggtc atacgcagta
    46501 gttcggacaa gcggtacatt ttaataattt aggggtttat tgatgagcga tatgcactcg
    46561 ctgctgatag cggcaatatt gggtgtggtc gaaggattga cagaatttct gccggtatcc
    46621 agcacgggcc atatgattat tgtcggtcac ttgttggggt tcgagggcga cacggcgaaa
    46681 acctttgaag ttgtgatcca gttaggatca attctggcgg tagtagtgat gttctggcgg
    46741 cgtctgtttg gcctgattgg catccacttt ggccgcccgt tgcagcacga aggagaaagc
    46801 aaaggtcgtt taacgctgat ccacattttg ctggggatga ttccggcggt ggtactgggg
    46861 ctgttgttcc acgacacgat taagtcattg tttaacccga taaatgtgat gtatgcactg
    46921 gtcgttggcg gtttgttgct gattgccgcc gaatgcctga agccgaaaga gccgcgtgcg
    46981 ccgggtcttg atgatatgac ctatcgtcag gcgtttatga ttggctgttt ccagtgtctg
    47041 gcgctgtggc ctggtttctc ccgttccggg gccaccattt caggtgggat gctgatgggc
    47101 gtgagccgtt acgctgcttc cgagttctcg ttcctgctgg cggtaccgat gatgatgggc
    47161 gcaacggcgc tcgatctcta caaaagctgg ggcttcctga caaccggcga tatcccgatg
    47221 tttgccgttg ggtttatcac cgcttttgtg gtggcgctga tagcgattaa aaccttcctg
    47281 caattgatta agcgcatttc gtttatcccg ttcgccattt atcgctttat tgtggcggct
    47341 gcggtgtacg tcgtgttctt ttgattgaga aaaggttcgg gagcaggcct ggttttctcc
    47401 ctcgctctgt agggagaggg ccggggtggg ggtgattgtt cgcaccggtg ctggtctgtt
    47461 cccctcaccc taaccctctc cccgaagggg cgaggggatg gttcgtgtta acgtttagtt
    47521 ttatactcag tcaggctttg ggcaacgttg ttccttccaa ctggctaccg ccgcaatccg
    47581 ccgtcgggtc agctcctcgc gaatctccac acctttaaat cccgcttcaa cgaccgcttt
    47641 tgtcggcact gactgcgcca cttcccaggc ttcgcgcaac caacggcctt gcgggtagtc
    47701 cgctgattca aaaccggttc tgccgcgcac gtcagcctcg ctggtcagcg ccagttgctc
    47761 gacacgctgc ggtttacgcc aggcatcgat ggaatcaaac aatttgacga tagtttttgg
    47821 gttcagcatt gggaaggtgt ggatgagatc gtgaaactca gccaccagtc tggctaaatc
    47881 gcgaatttca tttggcacgc gcagacgctg gcagagttgt tccactaatt tcacacccgc
    47941 cggaccgtga ccatgatgac gcggccaaag ttctggcggc gtcagtcctt taccgagatc
    48001 gtggcataaa gttgcgaaac ggacatcgac ctgcggactc agcatcgccg ccattgagag
    48061 cgtcattaag gtatgaatac ccgtgtcgat ttccggatgc cacctggcag gggccggaac
    48121 gccaaacagt gcgtcaattt ccgggaataa aacgcgcagt gcgccacaat cgcgcagtac
    48181 ctggaagaac acctgtggat tgcgggtggt gagggcgctt tccgtctctt tccatacccg
    48241 ttcaggcgtc aggtgttcca gttcacccgc atgggtcatc tcgcgcatca gcgtcagagt
    48301 ttcatcggca atacgaaaac cgaggtgggc ataacgcgca gcaaaacgcg ccacgcgcag
    48361 aacgcgtaac ggatcttcgc caaaagcggg ggaaacatgg cgcaacagac gattttgcag
    48421 atcgcccaga ccgttgtacg ggtcgataat ctcgccgtta tcgtcctggg ccagcgcatt
    48481 aatggtcaga tcgcgacgct taagatcatc ttccagcgtg acatccggtg cggcatagca
    48541 agtaaaaccg gtgtaaccgg aaccggattt ccgttcggta cgtgccagcg catactcttc
    48601 atgcgtttgc ggatgcagaa acacaggaaa atcgcggcct acctgctggt agcccgcgtc
    48661 gagcatctcc tgtggcgtac tgccgaccac cacccaatct ctgtctttga ccggtagccc
    48721 taacaatgca tcccgaacag caccaccgac cagataaatc ttcacgccat catcccttcg
    48781 tcaaatacac aattcctaat aataagacag tgcaacgaag aaggcgattt agttcatcca
    48841 gcgatctttg cgtttgcgcg ttgggatcag gtgtggcagt accagaccga gcagcaagcc
    48901 aagccccagc acgccaccgc catacataaa ccattgcatg atgatggtgc gctgtttgtc
    48961 atccaactgt acgctggcgg catcgacctt tttctgcgcg acaatcagct cgtttttcaa
    49021 tttctgattt tcttctttta atccgttgat cacgctgtcg ctctgcgcca ctttttgctg
    49081 catttctgcg gtgcgctgat tccaggtgtt atcgatattg gtgagtttat cggtcagggt
    49141 tttgacctga ttttccagat ctggcacacg ggagcgcagg cttggctcag tgctaagttg
    49201 tttcaacggg atccaggcgg tacggccaga gctgtctttc acctgggcat aattggtgtt
    49261 ggcgtcagtt tgtaataagg tcacttcctc gccggcgtta accgtgccca cgaggcgata
    49321 atgatctccc ggaccgctac ggacccaggt attcagttca tcagaaacat agcgcgtttc
    49381 ttcagcgtgt gagacggcag tggcgctaag tgcgagtaaa gttaatccga tcaggcgtaa
    49441 ttttggcatc aggctgtcgt tattgtcatg gaaagtggaa cgatagtagt ggcatcagtg
    49501 tcgctacgca aagcattcga catcaatcgg aatcctctgt gtcaggtctt gccactgctt
    49561 caaactttta cgcccgtcaa attgcccgtt gcatggcaat ttggcgcaaa atactatcta
    49621 ctgacaaaaa agatcgtatt aagttcgccg ttcatcgtca cttatgtgac ataaccgata
    49681 agtaaaggcc atggctcagg aaatcgaatt aaagtttatt gttaatcaca gtgccgttga
    49741 ggcgttgcgt gaccatctca atacgctggg cggcgagcac catgaccccg tgcagttact
    49801 gaatatttac tacgaaacgc cggataactg gctgcgtggg cacgatatgg gcttacgtat
    49861 tcgtggcgaa aacggtcgct atgagatgac catgaaagtt gcaggaagag tgacaggcgg
    49921 cttacaccag cgcccggaat ataacgtggc gttgagcgaa ccgacgctcg acctggcgca
    49981 gttaccgacg gaagtctggc cgaacggcga attgcccgcc gatctcgctt cccgcgtaca
    50041 accgctgttc agcaccgatt tttatcgcga aaaatggctg gtggcggtcg atgacagccg
    50101 aattgaaatc gctctcgatc agggggaggt gaaagcgggg gaatttgctg aacctatctg
    50161 tgagctggaa ctggaactgc ttagcggcga cacgcgcgcg gtgctgaaac tggcgaacca
    50221 actggtatcg caaaccggat tacgccaggg cagcctgagc aaagcggcgc gtggctatca
    50281 tctggcgcag ggcaatccgg cgcgtgaaat caaaccgacc accattttgc atgttgcggc
    50341 aaaagccgat gtggaacagg ggctggaagc ggcgctcgag ctggcgttag cgcaatggca
    50401 gtatcatgaa gaactgtggg tacgcggcaa cgatgcggcg aaagagcagg tgctggcagc
    50461 cattggcctt gttcgccatg cactgatgct gttcggtggc attgtgccgc gtaaagcgag
    50521 cactcactta cgtgatttgc tgactcaatg cgaagcgacc atcgcttctg cggtttctgc
    50581 cgtgacggcg gtctactcta ccgaaacggc aatggcaaag ctggcgttga ccgaatggtt
    50641 ggtaagcaaa gcctggcagc cgtttttaga tgccaaagcg cagagtaaaa tgagcgattc
    50701 cttcaaacgc tttgccgata tccatctttc ccgccatgcc gctgaactga aaagcgtttt
    50761 ctgccagccg ttgggcgatc gctaccgtga ccagttgcca cgtctgacgc gtgatattga
    50821 ctcaatactg ttgctggcgg gttactatga tcctgtcgtc gcgcaagcct ggctggagaa
    50881 ctggcagggg ctgcgtcacg ctattgcgac cgggcaacgc attgaaattg aacatttccg
    50941 taatgaggca aacaatcagg aaccgttctg gttgcacagc ggaaaacgtt aatcaggcaa
    51001 ggataaaatc gcttatgaag ccgctctctt caccgttaca gcagtactgg cagaccgttg
    51061 ttgagcggct gccagagcct ttagccgaga aatcacttag cgcacaggcg aagtcagtac
    51121 ttactttcag tgattttgtg caggacagcg tgattgcgca tccagagtgg ctgacggaac
    51181 tggaaagcca atcgccgcag gctgatgaat ggcagcatta cgcggcatgg ttgcaggagg
    51241 cgctcagcaa tgtgagtgac gaagccgggt taatgcgcga gctgcggcta ttccggcggc
    51301 gcattatggt gcgcatcgcc tgggcgcaaa cgctggcttt ggttactgaa gagagcatat
    51361 tgcagcagct cagccatctg gcggagacgc tgattgtcgc cgcgcgtgac tggctgtatg
    51421 acgcttgctg ccgcgaatgg ggaacgccgt gcaatgcgca gggcgaagcg caaccgctgc
    51481 tgattttagg catgggtaag ctgggcggtg gggaactgaa tttctcctca gatatcgatc
    51541 tgatttttgc ctggccggaa catggttgta cgcagggcgg acgccgggaa ctggataacg
    51601 cgcagttttt tacccgcatg gggcagcggc tgattaaagt gttggatcaa ccaacgcagg
    51661 atggcttcgt ctatcgcgtg gatatgcggc tgcgtccgtt tggcgaaagt ggcccgctgg
    51721 tgctgagctt tgccgcgttg gaggattatt accaggagca ggggcgcgac tgggagcgtt
    51781 acgcgatggt caaggcgcgg attatgggcg atagcgaagg cgtctatgct aacgagttgc
    51841 gtgcgatgct gcgcccgttt gttttccgtc gttacatcga tttcagcgtg attcagtcgc
    51901 tgcgcaacat gaaagggatg attgcccgtg aagtgcgtcg acgtggtttg accgacaata
    51961 tcaaactcgg cgcaggcggc attcgcgaaa ttgaatttat cgttcaggtg ttccagctca
    52021 ttcgcggtgg gcgcgaaccg tcgctgcagt cgcgctcttt actgccaacg ctcagcgcca
    52081 ttgccgcgct gcatctgctt tctgaaaacg atgctgaaca attgcgagtg gcatatctgt
    52141 tcctgcggcg tctggaaaac ctgctgcaaa gcattaacga cgaacaaacc caaacgcttc
    52201 cttctgatga gcttaaccgt gcgcggctgg cgtgggcgat ggactttgct gactggccgc
    52261 aactgaccgg ggcgctgacc gcacatatga ccaatgtgcg ccgggtgttt aatgaattga
    52321 ttggcgacga cgaaagtgaa actcaggaag agtcgctgtc ggaacagtgg cgtgagctgt
    52381 ggcaggatgc gttgcaggaa gatgacacta cgccagtgct ggcgcatctt agcgaggatg
    52441 atcgcaaaca ggtcctaacg ctgattgctg atttccgcaa agagctggat aagcgtacta
    52501 tcgggccgcg aggacgtcag gtgctcgacc atctgatgcc acatctgcta agtgatgtct
    52561 gtgcgcgtga agacgctgcc gttacgctgt cgcgcattac cgccttgctg gtggggattg
    52621 ttacccgcac cacctattta gaattgctca gtgaattccg tgcggcgctt aaacatttga
    52681 tttctctgtg tgccgcatcg ccgatgattg ccagccagct ggcgcgttat ccattattgc
    52741 tggatgaatt gctcgatcca aacactcttt accagccgac ggcgaccgat gcctaccgcg
    52801 atgagttgcg ccagtatttg ctgcgcgtgc cggaagatga cgaagagcaa cagcttgagg
    52861 cgctgcgtca gttcaaacag gcgcagctgt tacgcatcgc cgcagcggat atcgctggta
    52921 cgctaccggt gatgaaagtg agcgatcact taacctggct ggcagaggct atgatagatg
    52981 ccgtcgttca gcaggcgtgg gttcaaatgg tggcccgcta cggtaagcca aatcacctca
    53041 acgatcgcga aggccgtggt tttgcggtgg tcggctacgg caagctgggc ggctgggagt
    53101 taggctacag ttccgatctt gacctgatct tcctccatga ttgcccaatg gatgcgatga
    53161 ctgacggcga gcgggaaatc gacgggcgac agttttatct gcgtctggcg caacgcatta
    53221 tgcatctgtt cagtacgcga acctcttccg gcattttgta tgaagtggat gcacgactgc
    53281 gtccgtccgg ggcggcggga atgctggtga catccgcaga agcatttgct gattatcaga
    53341 aaaacgaggc ctggacgtgg gagcatcagg cgctggtgcg tgcgcgtgta gtgtacggcg
    53401 atccgcagct caccgcgcac tttgacgcag tgcgtcgcga gattatgacg ctgccgcgtg
    53461 aaggtaaaac tctgcaaacg gaagtgcggg aaatgcgcga gaaaatgcgc gctcatctcg
    53521 gcaataaaca tcgtaatcgc tttgatatca aagccgatga aggcggaatt accgatatcg
    53581 aatttattac ccaatatctg gtgttgcgct acgctcatga aaaaccgaag ttaacgcgct
    53641 ggtcagacaa cgtgcgtatt ctggagctgc tggcgcaaaa cgacattatg gaagagcagg
    53701 aagcgatggc gctgacccgt gcttacacga cgctgcgcga tgaacttcat catctggcat
    53761 tacaggaatt gccgggccat gtgtcggagg attgcttcac cgcagagcgt gatctggtgc
    53821 gggcaagctg gcagaagtgg ctggtggaag aatgaagtat ggtattatcg cgcgcaaatt
    53881 ttgaatctct caggagacag gaatgaaagt aacgctgcca gagtttgaac gtgcaggagt
    53941 gatggtggtt ggtgatgtga tgctggatcg ttactggtac ggccccacca gtcgtatctc
    54001 gccggaagcg ccggtgcccg tggttaaagt caataccatc gaagaacgtc cgggcggcgc
    54061 ggctaacgtg gcgatgaata tcgcttctct cggtgctaat gcacgcctgg tcgggttgac
    54121 gggcattgac gatgcggcgc gtgcgctgag taaatctctg gccgacgtca acgtcaaatg
    54181 cgacttcgtt tctgtaccga cgcatccgac catcaccaaa ttacgggtac tttcccgcaa
    54241 ccaacagctg atccgtctgg attttgaaga aggtttcgaa ggtgttgatc cgcagccgct
    54301 gcacgagcgg attaatcagg cgctgagttc gattggcgcg ctggtgcttt ctgactacgc
    54361 caaaggtgcg ttggcaagcg tacagcagat gatccaactg gcgcgtaaag cgggcgttcc
    54421 ggtgctgatt gatccaaaag gtaccgattt tgagcgctac cgcggcgcta cgctgctgac
    54481 accgaatctc tcggaatttg aagctgttgt cggtaaatgt aagaccgaag aagagattgt
    54541 tgagcgcggc atgaaactga ttgccgatta cgaactctcg gctctgttag tgacccgttc
    54601 cgaacagggt atgtcgctgc tgcaaccggg taaagcgccg ctgcatatgc caacccaggc
    54661 gcaggaagtg tatgacgtta ccggtgcggg cgacacggtg attggcgtcc tggcggcaac
    54721 gctggcagcg ggtaattcgc tggaagaagc ctgcttcttt gccaatgcgg cggctggcgt
    54781 ggtggtcggc aaactgggaa cctccacggt ttcgccgatc gagctggaaa atgcagtgcg
    54841 tgggcgcgca gataccggct ttggtgtgat gaccgaagag gaactgaagc ttgctgtagc
    54901 ggcagcgcgt aaacgcggcg aaaaggtggt aatgaccaac ggtgtctttg acatcctgca
    54961 cgccgggcac gtctcctatc tggcaaatgc tcgcaaactg ggtgaccgtc tgattgtcgc
    55021 tgtgaacagc gatgcctcca ccaaacggct gaaaggggat tcccgccccg ttaacccgct
    55081 cgaacagcgt atgattgtgc tgggcgcact ggaagcggtc gactgggtgg tgtcgtttga
    55141 agaagacacg ccgcagcgct tgatcgccgg gatcctgcca gatttactgg tgaagggcgg
    55201 cgactataaa ccagaagaga tcgccgggag taaagaagtc tgggccaacg gcggcgaagt
    55261 gctggtgctc aacttcgaag acggttgctc gaccactaac atcatcaaga agatccaaca
    55321 ggataaaaaa ggctaaccgg aaagcggttc acagatctcg cttcgtagca ggcaggccta
    55381 ttgacgctgt tgttttttca acctaaagta aaggaacaag ggtaagggag gatttctccc
    55441 ccctctgatc agttgttagt aagtcgggaa acttaacagt aacaacacaa ccagtatgat
    55501 gacgaacttc atcataaccc tttccttcta taaggcccct tcctcgggag gggctttccc
    55561 gtttcagccc cttgctgact ccccgattgt tgatgagcgc cggaccccgc gcaacgcttt
    55621 ccctgaagca gttttgcctt ccccgagcgg tcacgcaaaa cgctgcaacg tcctgagcat
    55681 ttgcaccttt tagcggaacg cagatataaa aaatccccgg ctttttgggc cagggatatg
    55741 tgttaatgaa accttcttga cgctgatttt ttttcaacct aaagtaaagg aacaagggtg
    55801 agggaggatt tctcccccct ctgattggct gttaataagc tgcgaaactt acgattaaca
    55861 acacaatcag tatgatgacg agcttcatca taaccctttc cttctgtaag gccccttcct
    55921 cgggaggggc tttcccgttt cagccctttg ctgactcccc gattgttgat gagcgccgga
    55981 cctcgcgcaa cgctttccca gaggcagttt tgccttcccc gagcggtcac gcaaaacgtt
    56041 gcaacgacct gatcatttgc agtttttagc ggaacgcaga tataaaaaat ccccggcttt
    56101 ttgtgccagg gacgttcata gctgaaaatt tattctacgt tttcggcgac gggagttgtt
    56161 gaggttaagg gtgaagtcaa tgccgccagt gagccgcctg aaacgccaat ttcattgagc
    56221 aaggagtcaa tcagcggtgc ctgtgtgcgg taagagagag cggctgataa tgcttgttcc
    56281 gccaggtttc cgccccccac attacccgtg tttgcatcac ccgcagcgct gccacgattc
    56341 agaccatcga cctgaataat cttgataccg tcaatagatt tcatcggctc aacggatttt
    56401 tctattaccg caggtagcgc ctgcaacaag gccagtttga atttaaggct ggtttgttca
    56461 tcagaaagta cgttgatagc atcgttcagc gcacgttgtg cttctgcttc cgccagaccc
    56521 tttttacgtg tagcttcggc taactcaacg atagccgccg cctgcatctc tgcggcttct
    56581 ttttctgctt tcgcccgcac ggtcagttca accgctttgg tttctgcatc ctgcgcggca
    56641 gcgattaggg caacttgttt agcacgatcg gcttctgcgg tctggcgagt ggtttctacg
    56701 ttttgttgcg cacttactgc ttctgcgagt gcaaggttag cacgcgcttc tgcctgggac
    56761 tgttgttccg atttggcggc aatagcgatc gatttcgtct ggttggcgat ttcggtgacc
    56821 tgctgttgtt cgatctcttt aatacgaact tcacgttcag cctcaacctt tcttgagcgg
    56881 acggcctgct cgcgctcgat ctccgtttcc tgaatttgtc gttcagccag aattcgcgtc
    56941 tgctctgctt cacgacgacg ttcagcttca aaagccgcaa tacgtgcatt ctgttcggca
    57001 gtacgggttt taacctgctg ctcctgctca agcgtcataa acgcttcttg ttgttcaatc
    57061 tccaacttgc gcgaaagtgc atcacggttt ttctcacgca ccgcaacttc tacatcctgt
    57121 tcaacttcgt tacgttcgcg acgacggcgc tccgtctcct gagtcagttt ggttaaacct
    57181 tcggcgtcaa aggcattgtt cggattgaaa tgttctttcg aggtctggtt aaagttggta
    57241 agtgaaacgc tttccatctc aagaccgttt ttcgacaggt cttctgcgac agtattttgt
    57301 actccctgca cgaagttctc gcgggtatcc tgtaactcat gcatggtcat ttgtgcagct
    57361 gttgcacgga gggcatcgac aaatttatct tcaaccaaca tacgtaagtc ttctggtgat
    57421 agcgtgcgtt gccccaacgt ctgcgccgca gtggcgatgc cttctacaga cggttttacc
    57481 cgtacaaaga aagcgactac tacatcaaca cgcatacgat ctttcgtaat caggctatca
    57541 atggttgagc ggctgacttc cagcttcaga gtattcatat tgatggggat tatttcatga
    57601 aagatcggca tcacgattgc gccaccgctc atcaccactt tttgcccacc taaaccagta
    57661 cgaacaaaag cttgctctgc cgaagcgcga cgatagagcc tggcgaaaat aattccaata
    57721 ataaacagaa tgcatacggc aataatcgcg gtaaacatcc aggagggcac agaattaaca
    57781 atatcatcca ttattaaatt tccttttgat taataaatta caatatttgt ggccagggat
    57841 tgttttccgc caaatatcgc gttgcagaaa gtcggcaaat aattaacact ttgtctcctt
    57901 tagtgaaaaa ttttccttct tcaggctcca gtagtaaata atgtatttgg ccaaattgat
    57961 cggtgagttt tccttcgcag ggattacctg aagtggcttg atgaccggta attaatgcca
    58021 tgctaccaat atattcttct tctgtaatag ctgaactatg gtcgcgtgga atccagggcg
    58081 caactatttt accggtataa tgcaccgcaa taatcgtaaa cagtaaactg acaggaacca
    58141 cgaacaggtt cgacagtggt gattgccaga ccatgatgca ggcatgctgc aacaggatgc
    58201 caataagacc aaaaaaacca gccagcaagc agagaacgac gagagctggc aatcttccga
    58261 tattgagata atgaagtgcc tggctaatat gacctgtggt tatggaatca taatggtcaa
    58321 gatgtgcatc aagagcaccg gagagcatgt gaccgcagat aagcgctaaa atttctaaaa
    58381 ggccgatcaa cagtacaaag gaaatagcga aaagataagg ggtattatag tcggcgaata
    58441 aaatcatatg tcactccaat gtccatttag tgacgcaata tttactttgg gatagtttcc
    58501 ttgcttacat ttatctttgt taataataac acatgttaga gaagttctgg aagagaaagt
    58561 tttttaatat ggttttttat ttttcactaa aaagtgtgat cggggacaat atatttacgc
    58621 acgttatgtt taaaggcact acactgattg gggaatactg aaatcagaaa aagcatatgg
    58681 ccttaaaagg tatgcgataa ccggaggatg tgcttatgga tcatagtctt aattctttaa
    58741 ataatttcga tttcctggcg cgtagttttg ccagaatgca cgcagaaggt cgcccggtcg
    58801 atattcttgc cgttactggt aacatggatg aagaacatag aacctggttt tgcgcacgtt
    58861 atgcctggta ttgtcaacag atgatgcagg caagagagct ggagttagag cactgatata
    58921 acgggcctga tggcccgttt tagtgtttgt attagatgct agttataggt gattttgagg
    58981 atgacgtctt tactaaacgg tccctcaaca acagtctctg ccggattcgc actaagttct
    59041 gccctgaatg gtgcggtgac cgaaggtgca ctagacgatg aaaaagaagc aaattgtgag
    59101 aagcggttaa attccgttgc tgttccatct ttatgaaata tcttcaatag cagtccatta
    59161 cccatatcca gatggctata atcactcaaa atatccgtag tgaaaaagct ccctaaaata
    59221 ttgaattgct cagtacaggc cgcaccagga tcttttgtca tgactacact aaacgtctca
    59281 ctaacgctgg tatttttaat tgttcgggaa tttattttac caaagtcaac aacgccatca
    59341 gctggttcca gtgcgacatt aacaaagcag tcaaggacat gaatattatt taatccacga
    59401 atcctgaagg tcaaattttt gtctacttct atagctcctg cgccactcat acctttaaac
    59461 tctagagcga tatattcatc aggtaacacc aggtttttat taactctatt ggcatttttt
    59521 gctaatttaa tatagagttg tacgcgagct ggaaaggtta ctgataattg aggctcagga
    59581 cctccactac agacagcggg gtttttatag tgattattaa aggtacccgc tccttttata
    59641 tcaatgcatt ctggtgctgc gatagcatta ggggcagtcg aatcataatc aactccattg
    59701 tatgttacgc caaaaatata ggagttctga ttataaaagt cctgaccgcc tggtaaaaac
    59761 atcattatat tagtccaggc tgatgggtat tcttctccag gtgatggggc aggtaatgca
    59821 ttattacaga agacattcat aaaatactca ctgctttgcc agatcttatc acctttttta
    59881 ccattactgg ggatagaaat tcttggtacg taaccaatga ctacggtatt tcccccttca
    59941 tgttcagcat agcagtccat tgccc
//
LOCUS       DAZFFI010000013        59641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFFI010000013 DAZFFI010000000
VERSION     DAZFFI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.013"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..337
                     /gene="yegR"
                     /locus_tag="ACHJ12_001081"
     CDS             20..337
                     /gene="yegR"
                     /locus_tag="ACHJ12_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO4988905.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(668..2029)
                     /gene="yegQ"
                     /locus_tag="ACHJ12_001082"
     CDS             complement(668..2029)
                     /gene="yegQ"
                     /locus_tag="ACHJ12_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4988906.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2132..2428)
                     /locus_tag="ACHJ12_001083"
     CDS             complement(2132..2428)
                     /locus_tag="ACHJ12_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4988907.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            complement(2430..2726)
                     /locus_tag="ACHJ12_001084"
     CDS             complement(2430..2726)
                     /locus_tag="ACHJ12_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4988908.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            complement(2935..3267)
                     /gene="yegP"
                     /locus_tag="ACHJ12_001085"
     CDS             complement(2935..3267)
                     /gene="yegP"
                     /locus_tag="ACHJ12_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4988909.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(3458..4180)
                     /gene="baeR"
                     /locus_tag="ACHJ12_001086"
     CDS             complement(3458..4180)
                     /gene="baeR"
                     /locus_tag="ACHJ12_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4988910.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(4177..5580)
                     /gene="baeS"
                     /locus_tag="ACHJ12_001087"
     CDS             complement(4177..5580)
                     /gene="baeS"
                     /locus_tag="ACHJ12_001087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4988911.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAGQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(5577..6992)
                     /gene="mdtD"
                     /locus_tag="ACHJ12_001088"
     CDS             complement(5577..6992)
                     /gene="mdtD"
                     /locus_tag="ACHJ12_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4988912.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQAVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(6993..10070)
                     /gene="mdtC"
                     /locus_tag="ACHJ12_001089"
     CDS             complement(6993..10070)
                     /gene="mdtC"
                     /locus_tag="ACHJ12_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4988913.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(10071..13193)
                     /gene="mdtB"
                     /locus_tag="ACHJ12_001090"
     CDS             complement(10071..13193)
                     /gene="mdtB"
                     /locus_tag="ACHJ12_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4988914.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(13193..14440)
                     /gene="mdtA"
                     /locus_tag="ACHJ12_001091"
     CDS             complement(13193..14440)
                     /gene="mdtA"
                     /locus_tag="ACHJ12_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4988915.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            14719..14775
                     /locus_tag="ACHJ12_001092"
     CDS             14719..14775
                     /locus_tag="ACHJ12_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4988916.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15047..15103
                     /locus_tag="ACHJ12_001093"
     CDS             15047..15103
                     /locus_tag="ACHJ12_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4988917.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            15376..15435
                     /gene="ibsA"
                     /locus_tag="ACHJ12_001094"
     CDS             15376..15435
                     /gene="ibsA"
                     /locus_tag="ACHJ12_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4988918.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            15656..16315
                     /gene="yegL"
                     /locus_tag="ACHJ12_001095"
     CDS             15656..16315
                     /gene="yegL"
                     /locus_tag="ACHJ12_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4988919.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            16312..17073
                     /gene="pphC"
                     /locus_tag="ACHJ12_001096"
     CDS             16312..17073
                     /gene="pphC"
                     /locus_tag="ACHJ12_001096"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4988920.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            17070..19010
                     /gene="yegI"
                     /locus_tag="ACHJ12_001097"
     CDS             17070..19010
                     /gene="yegI"
                     /locus_tag="ACHJ12_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4988921.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(19022..20374)
                     /gene="yegD"
                     /locus_tag="ACHJ12_001098"
     CDS             complement(19022..20374)
                     /gene="yegD"
                     /locus_tag="ACHJ12_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4988922.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            20508..21365
                     /gene="alkA"
                     /locus_tag="ACHJ12_001099"
     CDS             20508..21365
                     /gene="alkA"
                     /locus_tag="ACHJ12_001099"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4988923.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLAHAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDKL"
     gene            complement(21403..24720)
                     /gene="dgcE"
                     /locus_tag="ACHJ12_001100"
     CDS             complement(21403..24720)
                     /gene="dgcE"
                     /locus_tag="ACHJ12_001100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4988924.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            25038..25679
                     /gene="udk"
                     /locus_tag="ACHJ12_001101"
     CDS             25038..25679
                     /gene="udk"
                     /locus_tag="ACHJ12_001101"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4988925.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            25771..26352
                     /gene="dcd"
                     /locus_tag="ACHJ12_001102"
     CDS             25771..26352
                     /gene="dcd"
                     /locus_tag="ACHJ12_001102"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4988926.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            26374..28227
                     /gene="asmA"
                     /locus_tag="ACHJ12_001103"
     CDS             26374..28227
                     /gene="asmA"
                     /locus_tag="ACHJ12_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4988927.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(28501..30084)
                     /gene="yegH"
                     /locus_tag="ACHJ12_001104"
     CDS             complement(28501..30084)
                     /gene="yegH"
                     /locus_tag="ACHJ12_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4988928.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(30285..30434)
                     /locus_tag="ACHJ12_001105"
     CDS             complement(30285..30434)
                     /locus_tag="ACHJ12_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4988929.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            30743..31882
                     /gene="wza"
                     /locus_tag="ACHJ12_001106"
     CDS             30743..31882
                     /gene="wza"
                     /locus_tag="ACHJ12_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4988930.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            31888..32331
                     /gene="wzb"
                     /locus_tag="ACHJ12_001107"
     CDS             31888..32331
                     /gene="wzb"
                     /locus_tag="ACHJ12_001107"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4988931.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            32334..34496
                     /gene="wzc"
                     /locus_tag="ACHJ12_001108"
     CDS             32334..34496
                     /gene="wzc"
                     /locus_tag="ACHJ12_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4988932.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            34589..35428
                     /gene="wcaA"
                     /locus_tag="ACHJ12_001109"
     CDS             34589..35428
                     /gene="wcaA"
                     /locus_tag="ACHJ12_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4988933.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            35431..35919
                     /gene="wcaB"
                     /locus_tag="ACHJ12_001110"
     CDS             35431..35919
                     /gene="wcaB"
                     /locus_tag="ACHJ12_001110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4988934.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            35916..37133
                     /gene="wcaC"
                     /locus_tag="ACHJ12_001111"
     CDS             35916..37133
                     /gene="wcaC"
                     /locus_tag="ACHJ12_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4988935.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            37108..38325
                     /gene="wcaD"
                     /locus_tag="ACHJ12_001112"
     CDS             37108..38325
                     /gene="wcaD"
                     /locus_tag="ACHJ12_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4988936.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            38336..39082
                     /gene="wcaE"
                     /locus_tag="ACHJ12_001113"
     CDS             38336..39082
                     /gene="wcaE"
                     /locus_tag="ACHJ12_001113"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4988937.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASQAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            39098..39646
                     /gene="wcaF"
                     /locus_tag="ACHJ12_001114"
     CDS             39098..39646
                     /gene="wcaF"
                     /locus_tag="ACHJ12_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4988938.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            39672..40793
                     /gene="gmd"
                     /locus_tag="ACHJ12_001115"
     CDS             39672..40793
                     /gene="gmd"
                     /locus_tag="ACHJ12_001115"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4988939.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            40796..41761
                     /gene="fcl"
                     /locus_tag="ACHJ12_001116"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             40796..41761
                     /gene="fcl"
                     /locus_tag="ACHJ12_001116"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4988940.1"
                     /translation="MSKQRVFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            41764..42243
                     /gene="gmm"
                     /locus_tag="ACHJ12_001117"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             41764..42243
                     /gene="gmm"
                     /locus_tag="ACHJ12_001117"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4988941.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIAENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            42240..43463
                     /gene="wcaI"
                     /locus_tag="ACHJ12_001118"
     CDS             42240..43463
                     /gene="wcaI"
                     /locus_tag="ACHJ12_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4988942.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLSSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            43466..44902
                     /gene="cpsB"
                     /locus_tag="ACHJ12_001119"
     CDS             43466..44902
                     /gene="cpsB"
                     /locus_tag="ACHJ12_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4988943.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            45095..46465
                     /gene="cpsG"
                     /locus_tag="ACHJ12_001120"
                     /gene_synonym="manB"
     CDS             45095..46465
                     /gene="cpsG"
                     /locus_tag="ACHJ12_001120"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4988944.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            46520..47914
                     /gene="wcaJ"
                     /locus_tag="ACHJ12_001121"
     CDS             46520..47914
                     /gene="wcaJ"
                     /locus_tag="ACHJ12_001121"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4988945.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            47916..49394
                     /gene="wzxC"
                     /locus_tag="ACHJ12_001122"
     CDS             47916..49394
                     /gene="wzxC"
                     /locus_tag="ACHJ12_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4988946.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            49763..51043
                     /gene="wcaK"
                     /locus_tag="ACHJ12_001123"
     CDS             49763..51043
                     /gene="wcaK"
                     /locus_tag="ACHJ12_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4988947.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEEF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACKLTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            51040..52260
                     /gene="wcaL"
                     /locus_tag="ACHJ12_001124"
     CDS             51040..52260
                     /gene="wcaL"
                     /locus_tag="ACHJ12_001124"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4988948.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKP
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            52271..53665
                     /gene="wcaM"
                     /locus_tag="ACHJ12_001125"
     CDS             52271..53665
                     /gene="wcaM"
                     /locus_tag="ACHJ12_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4988949.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            53823..54818
                     /locus_tag="ACHJ12_001126"
     CDS             53823..54818
                     /locus_tag="ACHJ12_001126"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO4988950.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            55061..55954
                     /gene="galF"
                     /locus_tag="ACHJ12_001127"
     CDS             55061..55954
                     /gene="galF"
                     /locus_tag="ACHJ12_001127"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4988951.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGVKFRKGIEKLLSE"
     gene            56327..57412
                     /gene="rfbB"
                     /locus_tag="ACHJ12_001128"
     CDS             56327..57412
                     /gene="rfbB"
                     /locus_tag="ACHJ12_001128"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4988952.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPYPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            57412..58311
                     /gene="rfbD"
                     /locus_tag="ACHJ12_001129"
     CDS             57412..58311
                     /gene="rfbD"
                     /locus_tag="ACHJ12_001129"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO4988953.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWKIGVKRMLNELFTTTAI"
     gene            58369..59247
                     /gene="rfbA"
                     /locus_tag="ACHJ12_001130"
     CDS             58369..59247
                     /gene="rfbA"
                     /locus_tag="ACHJ12_001130"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4988954.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGNQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            59252..>59641
                     /gene="rfbC"
                     /locus_tag="ACHJ12_001131"
     CDS             59252..>59641
                     /gene="rfbC"
                     /locus_tag="ACHJ12_001131"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO4988955.1"
                     /translation="MNVIKTVIPEVLIFEPKKFEDSRGYFFESFNQRLFEDAVGKETL
                     FVQDNQSYSSKNVLRGLHYQSEPYAQGKLVRCIMGEVFDVAVDIRKESESYGQWVGVF
                     LSEDNNRQLWIPEGFAHGFLVKSDKAIF"
BASE COUNT        14659 a        15050 c        15880 g        14052 t
ORIGIN      
        1 tttaatgggg gaaggtgaga tgaaaaagat agctgctata tcattaatta gtatttttat
       61 tgtgtctggt tgtgctgtgc ataatgatga gacaagtatc ggtaaatttg gtcttgcata
      121 taaaagtaat attcagcgta aactcgataa ccaatactac accgaagccg aagcttcttt
      181 agccaggggc agaatatctg gtgcagaaaa tatagtaaaa aatgatgcag cccatttctg
      241 tgttactcag ggcaaaaaaa tgcagatagt tgacctgaag acagaaggtg caggattaca
      301 tggcgtcgct cgtctgacat tcaaatgtgg agagtgagaa tattttttgg taagcgtcaa
      361 acatgcgcgt tctggttgtg cttagccgga acctgtgcga gcacgatgcc gttacgtgaa
      421 aggcatcgtg ctatgaaggg agattctatc gatgtggtca atggaagacg gttaccagag
      481 atagggctta tgcataaaaa aataagcccg tgtaagggag attacacagg ctaaggaggt
      541 ggttcctggt acagctagca ttttatgggt tatgtttttc agcgaaacgg atgataacct
      601 taataaatac agctgtatgt gatcggtttc taagaatttt ccatccggga aaaataatcg
      661 aaattaatca cttaccgtgg ggattacgcg tggtttcccc ggagaaatta cgcatcagca
      721 gcgcgtaatt gagctcaaga tcctgcggga ccgggagcca cacagtataa ccatcgcctg
      781 gtgctatcgg catagcttcg cctttggcgt tttccatgtg ctcaagggta aaattaatgt
      841 tgccttgcgg cgtcatcagc tcaaggctgt cgccaacgga gaatttattt ttcaccgcta
      901 ccgccgcgag gtcccccttg cgctcaccgg taaactcacc aacaaactgc tggcggtcag
      961 aaactgaata accgtattcg tagttctgat aatcgtcgtg agtatgacga cgcaggaaac
     1021 cttcggtata gccacgatgc gccagacctt ccagagtttc cagcaggctg gtatcgaacg
     1081 gttttcccgc agcggcgtca tcgatagctt tgcggtaaac ctgtgcggtg cgtgcacaat
     1141 agtagaaaga tttggtacga ccttcgattt tcagcgaatg cacgcccatt ttggtcaggc
     1201 gttctacatg ggcgatggcg cgcagatctt tcgagttcat gatgtaagtg ccgtgctcat
     1261 cttcaaacgc ggtcatatac tcgcccggac gctgggcctc ttcgatcata aacactttgt
     1321 cggttggtgc gccgataccc agcgtcggct caacattttg caccggaatc ggctcgtact
     1381 tgtgtacgat gttgccaaca tcatcttctt tcccttcctg gacgttgtac tcccagcggc
     1441 aggcgttggt gcaggtgccc tggttcgggt cgcgcttgtt gatatagcca gagagcaggc
     1501 agcgaccgga gtaggccatg cacagcgcgc cgtgaacgaa gatctcgatc tccatatccg
     1561 gcacctgatt gcggatctct tcaatctctt ccagcgacag ctcgcgagag aggatcacgc
     1621 gggtcaggcc catttgctgc cagaatttca ccgtcgccca gttcacggcg ttagcctgca
     1681 ccgaaaggtg gatcggcatt tcagggaagt gctcacgcac cagcataatc agccctggat
     1741 cggacataat cagcgcatcc ggccccattt ccaccaccgg tttcaggtca cggataaagg
     1801 ttttcagctt ggcgttgtgc ggtgcaatgt tgaccacgac ataaaacttt ttccccagcg
     1861 cgtgggcttc attgatgccg agctgaagat tttcgtggtt gaattcgttg ttgcgcacac
     1921 gcagggaata acgcggctgg cccgcataaa cagcatctgc gccataagcg aaagcgtaac
     1981 gcatattttt cagcgttccc gccggggaaa ggagttccgg tttaaacata attctctcgt
     2041 tctgatgaca ggtcagatcc gcttcacctg atgaagcggt taggggagtg cccccacttt
     2101 aagggcgggc attgtagcgc aatcggcggg tttagctcca gtgtaaatgt ctggtggcgt
     2161 cctggttttg gttaaggata cgaatgacga taatttcccc tggtgattgc aggaaataaa
     2221 tgacgtggcg ttcaaaagga agaacaaaaa taccttcgcc taattcaggt ctgggcgtac
     2281 ctatattatt gttactcaga atctggagaa cgtctgaaag atgttccaca tatctgtcgg
     2341 cttgtggttc gccgaaatgg tgatagctgt aataccagat gccttctaaa tcctcattgg
     2401 cttttggcat gagtttaata atacgcacat tatttcctta tccctgcttt caccttccgt
     2461 aagaacgcgt ccttttccca cggctgagct tcaccactac tcaacccttc tgcgagcatg
     2521 tcacgtaacg cttgcaggcg agactctgct tgcttttctc gaagtaatcg cagtgactca
     2581 cggatcactt cgctttgggt gcgatagtcg ccggattcaa ttaaggactc gataaattca
     2641 cgcaactcat cgccgagatc aaccgtcatc gttctggcca ttttacgccc gcctgtaagt
     2701 aaaaatctta catcagtgta tgtcataaac cggatagcgt cacgaaagta ttagaagata
     2761 ttttggcaca tgaacatgtt caggaggata agtatgggat ggtaaacaaa gaaagcacaa
     2821 cgagtgctcg tcagtgccca ctattttaat ggcggcggtt actcttaatt attaataact
     2881 aagcgttatt tggatataaa gccggacgcc aacaacccac gcccggcatc gtaattacgt
     2941 attgtctttc accgtctggc ttgtgccatt ggctttaacg gaggcaattc cggtttcacg
     3001 agattgcgcg gtggcgtaca tctggctgga gccgataatt tgatgattag cggctttcag
     3061 attgaaatag aatttgccgt tacttgccgt ttttttctca tagcgttctt cttgcgggct
     3121 gttgctacgc accgacgcga tgcccttttc cgcagaggct tttgaggtat acagctcgct
     3181 ggtgaggata gtctcaccat tgcccgcttt tagcacaaac cggaactgat tatcactgct
     3241 cttactgagt tcaaaccaac cagccataat gactccttga ttgaaattgc gtatcgaaaa
     3301 cagcatctaa aggtgctgtt taccgattaa agtatgagcg attttctgat gcctgccagg
     3361 gcggagagat aatgtcagtt tgctgaccat gcgtgggata atgttgccgg atctggtctg
     3421 aacgccatct ccggctaaca aagtcatgtc gctaaaacta aacgatgcgg caggcgtcgg
     3481 cttcccagcg gtaaccaacg ccgtaaacgg cgcggataaa tgactgttcg gcgtcgagag
     3541 attccagctt gcggcgcagg tttttaatgt ggctgtcaat ggtgcggtcg gttactacgc
     3601 ggtagtcgtc ataaagatga ttgagcaatt gctcgcggga gaacactttt cctggttcat
     3661 gagagagcgt tttcaacaga cgaaattccg caggtgtcag gtcaagcatt ttaccgcgcc
     3721 atgaagcctg aaaacgacct tcgtcgataa tcaacgggct ttcagcatcc tgttgctgca
     3781 actcgcgctg cggtttgcaa cggcgcaaaa tggttttgac gcgcgctacc acttcccgtg
     3841 ggctgtacgg cttacagata taatcatctg cgccaatctc cagccccagc aggcgatcga
     3901 tctcttcgat ttttgccgtc accatcacga tcggaatgtc agaaaaacga cgaatttccc
     3961 ggcacagcgt caggccatcg gtgccaggga gcatcagatc taacaggatc agatccggtg
     4021 gtgtctggcg cacatacggc agtacctgat cgccgtggct gataagcgtc ggcgcatagc
     4081 tcgcagcacg cagataatca atgagcaact gccccagctt cggttcatct tccacgatca
     4141 aaatacgcgg tgtgttttcg tcgattggta actcggtcat acttctctct gtaaatcccg
     4201 ttccagcggt aactctactg taatgcttac cccgccaaaa ggcgaatggg cagcaataat
     4261 gcgaccatta tgtgcttcaa caatgttcag gcaaatcgcc agccccagcc cggaaccgcc
     4321 gctggcacgg ttgcgggaac cttcggtgcg ataaaaacgt tcaaacaatt tttgtagctg
     4381 atcgtcactg acacctggcg cactgtcggc aaaggtcagg cgcaccgttt tgtcgtgctg
     4441 cccggcagag attttcaggc tgccgccgct atcagtgtag cgcaggctgt tttcgagtaa
     4501 gttattgaat aactgcatta aacggtcgcg atcgccaaat acggtaatac tgtctggcag
     4561 ggaaaactgc agtttcaggc cacggctggc aaatcgttcg cgaaatgcgc cgcccgccac
     4621 ttccagcagt gggatcaaat ctaccggtgc tttttgatag gcgagagcgc cttcatcaga
     4681 catcgacaac tgatgaagat cgtcaaccag tttggtcagt gtaccgacct ccgcctgtaa
     4741 agacgccacc gtctccggtg tgaatttgcg cacgccatcc tgaatggctt ccagttcacc
     4801 gcgcagcacc gctaatggcg tacgcagttc gtgagaaata tcggccataa aatcgcgccg
     4861 catttgctgg tttttctcca gtgtgctggc aagctggttg aagtcttgcg ccagtttgcc
     4921 cagttcatct tcactggtgg gcgttacgcg ggtagtgaaa tcgcccgccg ccagtttgtg
     4981 cgtgccatcg acaagtcgtt ttaccggtgc cagtaaaccg cgcgccagca gaaaggtggc
     5041 gagcgccgcg agtaacgttg ccagagcgac aatcaaccag ctggtttgcc gctgttgttt
     5101 atcgaaattg atatcagtat tgcgcgttaa ccgctcaacg ggggaggcga tcaccgcgcc
     5161 aacttccgca ccgttgacca gaatgggtcg ccgtgtaccg tcaggtggaa tcggcgctcg
     5221 cggaccaacc agcactttgt tgttttgatc aaccacccag aactgggtac gccagccgtg
     5281 cggtggcata cccgggccgg gtttatcttc cgaattatcg tgttcaaatg aacgcaggat
     5341 ctgaaagaca aagcgatcat tgttgcgcag gaagcgccag ttgccatgct gcgcatactg
     5401 ctcgccaagc gcatcactta acagttgtaa tcgctgttca ttaccatgct tgatgtaatc
     5461 aataaagcca cgctcaaaac tgatacgcac cgcccagtgc atactgatca gcaagacaat
     5521 gcaggtggcg aaaatcgcca gaaacagttt gccggtaata ccgggtcgcc agaacttcat
     5581 tgcgcgctcc tttttcgccg cgaaatagct acattttgat gcgtatcgtt cggcactctg
     5641 gcaaagatga acgccggaag ggcgatgatc aacgccatgc taagccaggt gtacataaag
     5701 acggcttgtg tggtgccgct gtcgacgctg acatgctgcg aaccaaaaag tcccagcaac
     5761 agcccggcga tggtgacgcc gatactcatc gacaattgca taatcatcga cagcaggctg
     5821 ttgccgctgc tcgccagatt gtccgggaga tctttcagcg tcagggtgtt catggaggag
     5881 aaacgcgttg agttgaccat tccttgtaaa aacaggacga acggcaaaac gtagtaccag
     5941 cccaacagag cggtagtcat aaacaacagg gtgaccagcg acagacccag cgtggtcgct
     6001 accagtaccc gacgataacc aaagcgattc accacctgaa ccacaattcg cttcattccc
     6061 atgctgccaa gcaccatcgg gatcatcatc agtccggcat gaaacggtga gaaaccgagg
     6121 ccaatttgca ggaaaaccgg tgtcataaag ggcaacatgc cactgccaat acgtccggca
     6181 aagctccccg ccaggcccag cgaaaaggta cgagtgcgga acagtttcag actgaaaaga
     6241 gcgcggttgt tatttctggc gtgcagcaga taaagcacca gtgccaccac gccaactgcg
     6301 accaggcctg caatcgtcag cggcgataaa cctgtacctt tactgccgtc cagcgccagg
     6361 gttaataccg ccatgccaac cgccagcaat aaaaatccgg agagatcaaa gcgccgcgtc
     6421 tgcatggtgt agttcggcat taacagcaat gtggcgatcg caccgataat ccccaccgga
     6481 atgttgatca aaaagatcca gtgccacgac gcgtactcca ccagcagacc gccgagcgcc
     6541 ggaccgagca gcggaccgac ctgaccgggt aacgtgacaa aggtcatcgc cgccatatat
     6601 tgctcgcgcg gtacgatttt catcaccgtc aatctgccga ccggcaccat catcgcgccg
     6661 ccaacgccct gtaacgcgcg tgccagcaac agttcgttca gcgtgccgga aagcgcgcaa
     6721 aacagtgaac cgagagtaaa cagcacgatg gcggtaaaga aaatattgcg cacgccgact
     6781 ttgtccgcca gccagccgct ggcgggcagc atcaccgcca cggtcagcac ataagagaca
     6841 atgaccatgt gcatatgcaa cggactttcc ccgaggcttt gcgccattga gggaagggcg
     6901 gtgtttacga tggtggtgtc cagcgactgc ataaagaagc cgaaagccac aatccacaat
     6961 tgccagcggg tgctgtcggg aagatctgtc atttactcgg ttaccgtttg tttgggttta
     7021 cgcgaaaaac gcagccgcag acggtcgaaa aagagataca ccaccggcgt ggtatacagc
     7081 gtaaggagct ggctcattac cagtccgccg acaatggtga tccccagggg ttgccgcagc
     7141 tccgagccgt cgccgcccga caacaccagc ggcagcgcgc caaacagcgc cgccagggta
     7201 gtcatcataa tcgggcgaaa acgcagcaga caggcctgga aaatagcttc ctgcggcgtc
     7261 aggttaccgt gccgttgggc ttcaagcgca aaatcgacca tcataatggc gtttttcttc
     7321 acgatgccga ttaatagcat gatccctatc agggcgatta ggctgaacgg ggcattgaac
     7381 agctccagcg ccagcagcgc tccaacaccc gccgagggca gggtggagag aatcgtcagc
     7441 gggtgcacat aactctcgta aaggataccc agcacgatat acaccgtggc gatggcggcg
     7501 ataatcagga tcacctgtga gttcatcgtc tcctggaaca cctgcgctgt tccggcaaaa
     7561 ctgccgcgca ccgtcgaagg cacaccaagc tgggtcattg cgcgatcgat cgccgcactg
     7621 gcgtccgaga gcgattttcc ggtcggcagg ttgaacgaaa tggtcgaggc cgccgataat
     7681 ccctgatgat tcaccgatag tggggcattc gccggttgcc atttagcgaa atacgacagc
     7741 gggatcgctt ttccttcgtt attgataacg aacatttttt ccagcgcgct gatgtcctgg
     7801 gtatagcgcg gatccacttc catcaccact ttgtactggt tcatcggctg gtaaatggtc
     7861 gagatttgcc gctgaccgaa ggcgttattt aacagactgt tggcggcttg tacgtctatt
     7921 cccagccgtg ccatggtgtc acggtcgtaa accagattca tctccgcgcc gttatcctgc
     7981 tgatcggagt tcacgtccgc cagttccggc aacgtcgcca gttttttgcg gattttcggc
     8041 tcccattcgc gcagtgccgc caggtcgtcg gataacaacg tgtactggta gctggcgttc
     8101 gactgacgcc caccaacgcg aatatcctgt accgccatca ggaacagatt cgcccccggt
     8161 tctttcgcca gcttcacccg cagacggtcg ataatttgct gcgctgtttc gctgcgttcg
     8221 ccgcgtggct tgagggtgat aaacatcatc ccactgttca ctcgcgaacc gcctgtaaag
     8281 ccggtgacat tatccaccgc cggatcgtca cggataattt tcatgaaatc ctgcaactta
     8341 ccgcgcatcg cctgaaacga aatactctga tccgcctgaa tcccgcccat caacacgcca
     8401 gtgtcctgct ccgggaagaa ggttttcggg atcgagatat atagccagat attcagcgca
     8461 atggtgccaa gcagcaccac gcccaccaga cgggtatgat tgagcaccca tttcagtgac
     8521 ttgccgtagc cttgttgcag ggctaccaac atgcgaccaa aaccacgcag tcgcttttgc
     8581 tcgcgcggct tgctggcttt cagcatccaa ccacacatca ttggcgttaa tgtcagagaa
     8641 accagcaacg aaataccaat ggcgacagaa agcgtcacgg caaattcgcg taacagtcgg
     8701 cccggcaatc cgcccatcaa cagcagcggc aggaacaccg ccaccagtga cagactcatc
     8761 gacagcaccg taaaaccgac ttcgcgagta ccttgcagtg cggcttgcaa cggtttcatt
     8821 cccgcttcca gatgacgggc aatgttttcc agcaccacga tggcgtcatc caccacgaaa
     8881 ccagtagcga tggtgagcgc cattaacgaa agattattga gactgaaacc gcacaggtac
     8941 atcgccgcaa acgtaccaat cagcgaaacc ggcaccgcaa cggcgggaat aatagttgcg
     9001 cgacccgagc gcaggaataa aaacaccacc agaatcacca gcgccaccga gataatcagc
     9061 gtttgctcga cttcttccag cgaggcgcga atggtggggg agcgatcctg agcaatttgc
     9121 agatcaatcg ccgccggaat ggtttcctgc aactccggta attttgcccg gatgctgtca
     9181 accgtctgga taatattggc ttccggcagc ttgcggatca tcagcaaaat agccggtttg
     9241 gcattggtca tcccggcgtt acgtacatcc tgtacagagt cggtcactgt cgccacatcg
     9301 cccagacgaa ccgcgccgcc gttgttgtaa tgaataatca gcggctgata ttcggcggcg
     9361 gtttttagct catcattggt ctggatctgc cagcggtgag tgccatcttc cagcgccccc
     9421 tgcggtttac gcacgttggc attgctgatg gcagtgcgta cgtcgtccag cgagacgccc
     9481 tgattaaaca gcgcctgcgg attcagcccg acgcgtacgg cgggcagtga gctgccgccg
     9541 acatcgacat caccaacacc gtcgatttgc gaaatcgtcg gagccagctg cgtcgaggcg
     9601 aaatcgtaca gttcaccctg cgaataagta tcggatgtca gcgtgaggat cataattggc
     9661 gcatccgacg ggttagcttt gcgataggtc ggacggctgg gcatcccgct gggcagcaaa
     9721 ctttgtgcag cgttgatcgc cgcctgcaca tcgcgcgccg cgccgttgat atcccggtca
     9781 aaatcaaact gcaaaataat acgcgtgctg ccgagcgaac tgctggaggt catttcactg
     9841 actccggcaa tgcgcccaag tgagcgctcc agcggcgtgg caacggaaga cgccattgtt
     9901 tctggtgacg caccgggcag cgaggcgctg accataatca ccggaaaatc gacctgcggc
     9961 agcggggcga ccggcagcat acggaagccc agtatgccgc acagggtaat ggcaaccgac
    10021 agtaaaatcg tcgccaccgg acggtaaatg aagagggcaa aaaacttcac ttacgcctcc
    10081 tcttcatggc gagcaaagcg acttttggtc cacaatgcca ggcggtcgaa cagcagataa
    10141 atcaccggcg tggtaaacag cgtcagcacc tggctgacaa tcaggccgcc gaccatgccg
    10201 atacctaacg gacgacgcag ttccgcgccg accccggtac tcaacatcag cggcagcgcg
    10261 ccaagcagag ccgccagagt ggtcatcagg atcggacgaa aacgcaacag acaagcctgg
    10321 tagatggcat cgcgcggcga catgccttgc tcgcgctcag cagccagcgc gaagtcgatc
    10381 atcatgatgg cgttcttctt cacgataccg atcagcaaaa taatgccgat aatcgcaatc
    10441 acatccagtt cgctaccggc aatcattaac gccagcagtg cgccaacccc tgcggtgggt
    10501 agcgtcgaga gaatggtgat cgggtgaata aagctctcgt acagaatgcc gagcacgata
    10561 tacatcgcca ccaccgccgc gacaatcagc cagacagtgc tgccaagcgc cgactggaag
    10621 gcgagggtgc tgccctggaa ctgcgtggtg atatccaccg gcagattcag cgtcttctcg
    10681 gtgtccataa tcgcctgcac cgcatcgccc agcgaatagt tatccggcac gttaaaggag
    10741 atggtcgtta ccgggaactg atccagatgg ttaatggaaa gcggcgcaaa acgctgttca
    10801 actttggcaa ttgagcttag cggcaccacg ccgccgtcgc tgctggtcag gcgaatggta
    10861 tccagcgccg cgaggccagg agtattttcg gtgttgtgct ccagcaccac gcgatactgg
    10921 ttagcctgag tataaatagt ggaaatcagt cgctgaccaa acgcgttgta cagggcgtta
    10981 tcgacatccg ccatgctgat ccccagacgg ctggcgctgt cgcgatcaac attgacatac
    11041 gccaccagcc ctttgtcctg ccagtcgctg gagacatcag aaagctgtgg cagttgctgg
    11101 agtttttcca tcaactgtgg cacccaggta ctgagcgcat ccagtgaggt ggcctgcaag
    11161 gtaaactggt actgggtgcg gctgacctga gtatcgatgg tcaggtcctg cgttggttgc
    11221 aggaagagat cgacgcccgg cactttatct accgccgttt gcagacgggc gatgactttt
    11281 tgcacccgat catcacgttc atccaacggt ttgaggttga tttgtaaacg cgcactgttc
    11341 agcgacgggt tagtgccatc aacgccaaca aatgaggtca ggctttgcac cgccggatcc
    11401 tgcaaaatca cgtccgcgac ctggcgttgc cgctgggcca tattggcaaa ggagctggat
    11461 tgcggtgcct gcaaagtgcc ctgaataatg ccattgtcct gtaccgggaa gaaacctttc
    11521 ggaatgaaca cccacagcag cacgctaagc agcagcgtgc tgagcgccac gcttaaggtt
    11581 agccacggat gattcagcac tttcgccagt ccacggccat aggcggcgat tatcctgtcg
    11641 aacatttttt ccgaggcacg ggagaagcgg ttctgtttac gcaacgactc ctggctgagc
    11701 atccgcgcgc acatcatcgg tgtcagggtc agcgacacca ccgctgagat caaaatcgct
    11761 accgccaggg taatagcaaa ttcgcggaac agtcgcccga cgatatcgcc cataaacagc
    11821 agtgggatca acaccgcaat cagcgagaag gtcagcgaga taatggtaaa gccgatttca
    11881 cccgcgcctt tgagcgccgc cgccaacggt ttttcgcctt tttcgatata gcgggaaata
    11941 ttttcgatca ctacgatggc gtcatcgacc acgaacccgg tggcgatagt taacgccatc
    12001 agcgtcaggt tattgattga aaaatcgaga aacaccataa ccgcaaaagt gccgattaac
    12061 gacagcggca cggcaacacc gggaatgatg gtcgccggaa tattgcgtaa aaacaggtag
    12121 ataatcatga ctaccagcgc gatagccatc atcaattcaa actgagtatc atcgacggat
    12181 gcgcgaatat tggtggtgcg gtcagaaagt accgtcacct tcaccgattt cggcagactc
    12241 tcagtcagct gtggcagcat ctgccggatg ctgtcggcgg tggagataat gttagcaccg
    12301 ggctggcgct gaacattcat cacaatggcc tgttctttgt tcgcccacgc gccgagccag
    12361 ctgttttctg caccttgctc tacggttgcg acatcgccca gacgaatcgg cgcgccgttc
    12421 tggtaggcga tgattagctg gcgatactct tcggcggatt gcatctggtc gttagcggaa
    12481 agcgtgaccg cacgggaagg gccgtcgagg ctaccttttg ccgagttaac gttagcgccg
    12541 gtaatggcgg tgcgcacggt ttcgctggtc aggccgaggg cggcaatcgc ctgggcgtta
    12601 agtttgacgc gaacagccgg acgctgaccg ccggaaagcg tcaccagtcc gacaccggaa
    12661 atctgcgaga ttttctgcgc gacgcgggtt tccaccatat cttccacttg cgtcatcggc
    12721 atggcggttg aggtgacggc gagcgtcatg atcggcggat ctgccgggtt cactttgctg
    12781 taaaccggcg ggttaggcag atcgctcggc aacaagttgg tcgcagcgtt aatcgcggcc
    12841 tgcacttcct gctcggcgac atcgagcggt aatgttagct ggaactgcaa agtgataact
    12901 gacgcaccgc cggaactttg cgacgacatc tgtttcaggc cagacatctg cccgaactgg
    12961 cgttctagcg gcgcggtaac ggcagaggtc atgacatccg ggctggcacc tgggtagagc
    13021 gtgaccacct gaatggtcgg atagtccact tccggcagcg ccgaaacggg cagggcgcga
    13081 taaccgataa tcccggcgag taagatcgcc accatcagca gcgtggtggc gacaggacgc
    13141 ataataaaca ggcgcgacgg gccgcctgtg ctgctcgggg gtaacacctg catcaggagc
    13201 gtgctccttt tttcgcgtat tcgcggctgg tggctttctc ttccggagta gtggcgctct
    13261 gggcttccac tacttccact ttcgcccctt cggtcaggcg atcaatgccg tctgtcacca
    13321 cgcgatcgcc cgcagaaata cctgcacgga tcaccacttt ctgactgtcc tgaatgcccg
    13381 gcgtcaccag atgtttgctg actttgtttt cgctattcag cacccagaca aaatggcctt
    13441 cattgcccat ttgcagggcg gctgttggga tcactacggc gttttgttcg gtgtcgacta
    13501 acatgcgcgc gttaacaaac tgattgggaa acagcgcatc atcctgatta ttaaagcgtg
    13561 ctttcacttt aatcgtaccg gttgtggcat cgatttggtt atcgaggctt aacagcgtgc
    13621 cttcacttaa tttcttcgaa ttggtgcgat cccaggcttc taccaccagc ggttttccgg
    13681 ctttttgcgc ctgcactaca gtagcgatat cgctttccgg cagggtaaag actaaatcga
    13741 taggatgcgt ctgggtgatc accacaattc cggtggtatc accactggag atttggttac
    13801 caacatcaac ctgcttgaga ccaacgcgac catcgactgg tgcggtgatg cggctccagt
    13861 cgagttgcag ctgcgcgctg gcgacgcttg cttcatcagc cttaatggtg ccttcggttt
    13921 cactgaccag cgcctgttgg gcatccagct cctgacggga gacgagattg gtttttgcca
    13981 gttgctgata acgcgccaga tcacggcggg cgttagcaag cgtggcttta tcttttgcca
    14041 gttggccctg ggcttgtgct aatgcaactt tgaactggct ggggtcaatt tctgccagta
    14101 aatcgcctgc tttgacctgc tggccttcct ggaaatgtaa cgccatcagt tgaccgtcca
    14161 cgcggctgcg caccgtaaca gtattagcgg cggtaatggt gccaagcccg gtgaggtaac
    14221 gcggaactgc ctgttctacg gcggtcgccg cctgaaccgg ggctaatggg ccggaacgca
    14281 taccacggcg accacccgct ggcgattgct gcgcttgttt cgtcgcccct ggggctgcac
    14341 tctgggagtc attgcggcct tgccagaacc agaatgcggc gatggcggcg ataaccacca
    14401 cgattacgat tacccaacgg gatttataac tgcctttcat cgttaagagt ttctcttcct
    14461 gaaacgtttc gcggaatgat actagtgtag ccagccaata agggagaaaa atggaggaat
    14521 tatgaaacac tatcgggaat cgtctgaatg gcgggcacat ttgcgagcac gcatccagta
    14581 ataacacagg aaactatttt atctacgcgt tagcgataga ctacatacag ggcgaaagga
    14641 ggtaagccgc agatttcagc cggatgctga aacgggaaag cccctcccga ggaaggggcc
    14701 ttaaataagg aaagggtcat gatgaagcta ctcatcatcg tggtgctctt agtgattagc
    14761 tacccagcat actaagacta ccagggcggg ggaaaccccg ctctaccctc actcctgaaa
    14821 gtatgccttc acgataagat tgtcaatccg caggctttgc cgtctgcgat cctgccagca
    14881 aaaattcttt gcgagacgtt acgcaataat cacactggaa actattttat ctgcaagtca
    14941 gcgatagact aagtgcatgg cgaaaggagg taagccgacg atttcagcgg gacgctgaaa
    15001 cgggaaagcc cctcccgagg aaggggcctt aaataaggaa agggtcatga tgaagctact
    15061 catcatcgtg gtgctcttag tcataagctt ccccgcttac taagactacc agggcggggg
    15121 aaaccccgct ctaccctcac tcctgaaagt atgccttcac gataagattg tcaatccgca
    15181 ggctttgtag tctgcgatcc tgccagcaaa tattctttgc gagtcgttac gcaataatca
    15241 cagaggaaac tattttattc acgcgttagc gatagactgc attcagggcg aaaggaggta
    15301 agccgatgat ttcagcggga cgctgaaacg ggaaagcctc tcccggagaa gagggctttt
    15361 aataaggaaa gggttatgat gaagcacgtc atcatactgg tgatactctt agtgattagc
    15421 ttccaggctt actaagaaca ccagggggag ggggaaacct ctccctaacc ctcactcctg
    15481 aaattatgtc actccgccct tagtgtcaat cgacggctct ccgttcttgc gaggcgaata
    15541 gcaaaatttt ctgcgccgcc gggcctaatt ctgaaagttt cttcgtttgc tcttatttca
    15601 ttattgcagt cggtatgata ccggacctcg ataattctct aagaaaggat ttcttatgag
    15661 cgaacaaatc acgtttgcca ccagcgattt tgccagtaac ccggaaccac gttgcccctg
    15721 cattttgctg ctggatgttt ctggctcgat gagcggcaga cctattaatg aacttaacgc
    15781 cggattggtg acctttcgtg atgaactgct tgccgatccg ctggctttaa aaagagtgga
    15841 actcggcatt gtgacgttcg gcccggtgca tgtggaacaa ccctttacca gcgccgctaa
    15901 tttttttccg cccatcctgt ttgcccaggg cgatacacca atgggtgcgg ccattaccaa
    15961 agccctggat atggtagagg agcggaaacg tgagtatcgt gcaaatggta tttcgtatta
    16021 tcgcccgtgg attttcctga ttaccgatgg tgcaccaacc gatgagtggc aggctgcagc
    16081 caacaaagtg tttcgggggg aagaagataa aaggtttgct ttcttttcca tcggtgttca
    16141 gggggcggat atgaagactc tggcacaaat cagcgttcgt cagcctttgc ctctgcaagg
    16201 attacaattc cgtgaactgt ttagctggtt atccagttcg ctgcgttcgg tttcccgctc
    16261 cacgccggga acggaagttg tgctggaagc gccaaaaggc tggacgtcag tgtgagctgg
    16321 cgtctggttt atgcatccgc tgttggaaca tcgcacatca gcgcggactt gccttgccag
    16381 gatgcctgcc agatgcaggt tgcctggcta aatgaccagc agccattact ggtgatgttc
    16441 cttgctgatg gcgcaggtag cgtttcgcag ggaggagaag gcgcgacgct cgccgtcaat
    16501 gaagcgatgg cttatatgtc gcaaaaagtg cagggtgggg aattggggct taatgatatc
    16561 ctcgccacgg atattgtgct gaccgttcgt cagcgacttt ttgccgaggc ggaagccaaa
    16621 gaattagcag ttcgcgattt tgcctgcacg tttctgggat tgatttcatc ggctaatggc
    16681 accttgatta tgcagattgg cgatggcggc gtggtggtgg attttggtca cggtctgcaa
    16741 ttgccgctta cgccgatggt gggtgaatat gccaatatga cgcacttcat taccgatgaa
    16801 gatgccgttt ccagactgga aactttcacc agcactgagc gtgtgcataa agttgcagca
    16861 tttacggatg gtatccagcg actggcgtta aatatgctgg ataattctcc tcatgtacct
    16921 ttctttaccc cgtttttcaa tggactggcg tcagcaacgc aggaacaact cgatttatta
    16981 cctgaattgt taaagcagtt tttgtccagc ccggcggtaa acgagcgtac ggatgatgat
    17041 aaaacgttgg cactggcgct gtggttacca tgaaacccac tttatatact gctactggtg
    17101 agtgcgttac gccaggccgt gaactgggca aaggcggtga aggcgcggtt tatgatatca
    17161 atgagtttgt cgatagcgtc gccaagattt atcacacgcc gccacccgcc ttaaaacagg
    17221 acaaacttgc ctttatggct gcgacagctg acgcgcaatt gttgaattat gtcgcctggc
    17281 cgcaggcaac gcttcacggt ggacgaggcg gaaaagttat cggttttatg atgccaaaag
    17341 tttctggtaa agaaccgatt catatgatct atagcccggc acatcgtcgt caacgttacc
    17401 ctcattgtgc gtgggatttt ctactctatg ttgcgcgcaa tattgcttca tcttttgcta
    17461 cggttcacga gcacgggcac gtcgtgggtg acgtaaacca gaacagtttt atggtaggtc
    17521 gcgacagcaa agtggtactg attgatagcg actcctttca gattaacgcc aatggcacac
    17581 tgcatttatg cgaagtcggc gtgtcgcatt ttacgccgcc agagctacaa accttgtcgt
    17641 catttgtcgg ctttgaacgc acagaaaatc acgataattt tggccttgcg ttgctgattt
    17701 ttcacgtctt gtttggtggc cggcatcctt attctggtgt gccgcttatc tctgatgcgg
    17761 gtaatgcgct ggagacggat attacccatt tccgttatgc ctacgcgtca gataatcagc
    17821 gacgtggttt aaaaccgccg ccacgatcga ttccgctatc gatgttaccg ggcgatgttg
    17881 aagccatgtt tcagcaggca ttcacggaaa gtggcgtggc aaccgggcgt ccgacggcta
    17941 aagcgtgggt agcggcactt gattctctgc gccaacagtt aaagaaatgt cccgtttcgg
    18001 caatgcatgt ttatcccgct catttgaccg actgcccgtg gtgtgcgctg gataatcaag
    18061 gcgttatcta ttttattgat ctcggcgaag aggtcattac caccggcggt gattttgtgc
    18121 tggcgaaagt ctgggcgatg gtgatggcgt cagtagcacc gccagcattg caattgccat
    18181 tacccgatca tttccaaccg actggcaggc cgcttccttt aggcctgtta cggcgcgaat
    18241 acatcattct gattgagatc gcactgtcag cgttatcgct gttgctttgc ggccttcagg
    18301 cagaaccgcg ttatattatt ttggttcctg tgctgtcggc tatctggatt attggcagtc
    18361 tgacaagcaa agcgtacaaa gcagaaatcc agcaacgccg tgaggcattt aatcgcgcga
    18421 aaatggacta tgagcattta gtcagccaga tccaacagtt gggcgggctg gaaggtttta
    18481 tcgccaaacg ggcgatgctc gaaaaaatga aggacgaaat tctcgggtta ccggaagaag
    18541 agaaacgcgc tctggcagca cttcatgaca ctgcaaggga acggcagaag cagaagtttc
    18601 tggagggatt ttttattgat gttgcctcta ttcccggcgt tggccctgcg cgtaaagcgg
    18661 cgttacggtc ttttggtatt gaaacagccg cagatgttac ccgtcgtggc gttaagcaag
    18721 tgaaagggtt tggcgaccat ctgacccagg cggttatcga ctggaaagcg agctgtgaac
    18781 gccgttttgt gttcaggccg aatgaagcga taacgccagc agacagacaa gcggtaatgg
    18841 cgaaaatgac cgccaaacga catcggctgg aatcgacgtt gactgtcggc gcgacagagt
    18901 tgcagcgatt ccgccttcat gctccagcac ggaccatgcc gttgatggaa cctttacgtc
    18961 aggcggcaga aaaactggct caggcgcagg cagatttaag ccgctgctga gccattttct
    19021 tttaacgaaa caccacttcc gcccagcgtg ccagcccggc ggtgacggag ccaaagtcat
    19081 cgccgcctgc aatcggaatt cccggcaact gttctgccag cgctttttta atcagcggcg
    19141 aacgggcgct accgccggtc aggtagataa cgtccggttt ttcctgggcg ttatccaggg
    19201 ccagttgcac ctgttccaga atccgcgcca gtggctggct gagggcgctt tccagccctt
    19261 gctggctaat cagcgtagcc agttcatcgc tgataaacgg cagtgaggcg cgggtttccg
    19321 ctacgcttga aagagcaatt ttgcactctt ctgcgctacg caccaggcga tagcttaaac
    19381 gctgacgcca gactttctgt agcagggcca ctttttccgg ttcgcgggca tcgcgtacca
    19441 gatcgttaag cagacgaccg ttggcactac tgtagaaatc actctgcgca ggtacgtcgt
    19501 tgatggcaac cgcattccac cacggcagaa tcggcagggc gatgcctttt tcggtttcgc
    19561 cacccatgcc cagtaacggc atcaggtttt taaacgccag tgcgatgtcc agatcgttac
    19621 cgccaatacg gcaaccactg tgacccagca ggctggcttc acgatcgaga cgcgaacgcc
    19681 actgcggccc catcagcagc aatgaacagt cagtcgtacc accgccgata tccaccacca
    19741 gcacccgttt ttcttcctgc aaggtggctt cgtaatccag cccagccgcg accggctcgt
    19801 actggaatac cacgtccctg aatccggcac gcttcgccgc gcgttccaga atcccttgcg
    19861 cctgggcgtt tgcttcatcg ccgcccagcc cctggaagtt gatcgggcga ccaatcaccg
    19921 cctgcgtaat cgcttctggc agctgcgcct gcgcctgctg gcgaatgtgc aacatcattg
    19981 cgcagaccag atcctcaaac agcgctacct gctgcggttt taagccgctg gcaccgagga
    20041 acgattttgg tgatttcaca aaccacactt cttctggatc atcaatgtac tgtgccagtg
    20101 aggaaagacc gaactgcacg cttttcgccg taacatcgat atcttcttcg cggttataac
    20161 gaatcgcccg acgtaacagc gcctgcgttt catcgtcgtc tgccggaaca tcatgatggc
    20221 ggtacagcca ttcgcttacc gcttcacgcg ttggcgcgca aagcattgaa ggcagcagcg
    20281 tgctgtcgtt ttccattttt agcaagtgcg gtttaccgtc acgcatgacc gccactgaac
    20341 agtttgctgt accgtaatca aaaccaataa acacgaaata atccccatgc cggtgaagaa
    20401 ggggcgtgac tttagcgaaa tgttgccgtc gcgacaaccg gaatatgaaa gcaaagcgta
    20461 acgtctgaat aacgtttatg ctgaaagcgg atgaataagg agatgcgatg tataccctga
    20521 actggcagcc gccgtatgac tggtcgtgga tgttgggatt tctcgccgcc cgtgcggtga
    20581 gcggtgtgga aacggtcgct gacgattatt atgcccgtag tctggcggtg ggcgaatatc
    20641 gcggcgtagt gactgctatt ccggatatag cccgccatac tctgcacata aatttaagtg
    20701 caggtttaga acccgttgcc gctgagtgtc tggcgaaaat gagccgcctg cttgatctgc
    20761 aatgtaaccc gcagattgtt aacggtgcgt tgggcaagtt aggcgcgggg cggcccggat
    20821 tgcgtttacc cggctgtgtt gatgcttttg agcagggcgt gcgggcgatt ttaggccaac
    20881 tggtgagtgt ggcgatggcg gcaaaattga ctgccaaagt ggtacagctt tatggcgaac
    20941 ggctggatga ttttccggag tatatctgct tccccacgcc tcagcggctg gcagcagccg
    21001 acccgcaggc attaaaagcg ttaggtatgc cgttgaaacg ggcagaggcg ctgattcatc
    21061 tggcacatgc ggcgctggag ggcaccttac caatgacaat accgggcgat gtggagcagg
    21121 cgatgaaaac gctgcaaact tttccgggta tcgggcgctg gacggcgaat tatttcgcct
    21181 tgcgtggctg gcaggcgaaa gatgttttcc tgccggatga ttatctgatt aaacagcgat
    21241 ttccgggaat gacaccggca caaatccgcc gttatgccga gcgctggaag ccctggcgtt
    21301 cttatgcgct gttgcatatc tggtatacgg aaggctggca accagacgga acggacaaat
    21361 tgtaggcctg atacgtcgta tcaggcgatg gggaagcacg cctcagttaa tcgcgaaata
    21421 actgctattc accagcaaat ccagcggctg ggcatcggca atcacatcac cataaatcag
    21481 atcgacgccg atgccagaaa gcgtatccat cactaagggt aaaacgactg gcccggcgat
    21541 ggttttcatc ccgagtcgct gagcgtgccc ctgaataatc gtaatcagca tctcatccat
    21601 caaattaccc tgcacgttgg cgcataactc accatcaagt agcaggtaat ctgccatatt
    21661 cgctttcagc gagttgaaga tttgcagatc gcggcccacc tggctgagca ctatccgaca
    21721 tcccgccagc cgcagttttt gcacgctttc tgcgtgatct aaaatcgcgt ctgccggaat
    21781 aatcagatgt aataaccgtg gtggtagagg gctattttcc agctgctcaa gcagatcatt
    21841 caccagcgtg gcgctactca aaccggcaac ggaaaggggg agggcgatgc ttatgccttt
    21901 actggcaacc gcttttgcgg ccagctggaa aaattcgtgg aatacccgtc ggtcaagagc
    21961 atggctaagt gccggatcgc tgaagctacg acgaaatttt tgttcatcaa taatctcgcc
    22021 ttcgcaactc cagagtttaa gcgaaatcag ccacaaatta cgcgcttccg ggatccgcgg
    22081 cgaagcgaca ccgtgggcca tcatcatcaa ctgattttct ttaatcatcc gccactgttc
    22141 atcaagcgac atcgccgccc gttcgctatg tgcggcagct tgctgtggtt cgtaaaccgt
    22201 cacccggccc cgtccaccat ttttggaggc ataacaggcg atatcagcct gcgacatcac
    22261 ttctgccgcc tgatgattgt tgtcatcaat caaggtaatc ccggcactgg cacctacccg
    22321 atgcacacgg ccttcccata taaagtgata gtcattcacg gcactgataa tgcgtgtagc
    22381 gataaaacgc gcgctttcaa cattacagtc tggtagcagc agaccaaatt catccccacc
    22441 gagtcgcgcc agcacgtcgc tggaacgcag catactcagc atcaacgacg ccagttcgcg
    22501 cagcaaagcg tcgcccgccg catgcccggc gctgtcattc accgctttaa agcgatcaag
    22561 atcgataaac accagggcat gtcgctgatg tgtactgttt accgtttgca gcagcatacg
    22621 cagttgcttc tcaaaactgg cgcgattggc gagatgcgtc agtgcatcat gggaggcgct
    22681 gtagctcagc tggcgcagca ttttgcgtga ttcggtaacg tcctgaatca ccagaaccga
    22741 accaatattg ctgccgtcca gagtacttaa cggcgtaata ctgtaatgca cgtcgtagct
    22801 gccgccgctc cggcagtgca acaccacatc ttgttcaatc gcggaacgtg aggtgtcggc
    22861 actgtaaatg ttctccatca ggggaccatt gtcgccaaag gtaatatgca acaccgtcag
    22921 gagcggaaca cctaatgctt cttcctgcgt ccagccgctc atcttctccg ccactggatt
    22981 cataaaggta attttcattg ccatatcaat acagaccacg gcttcgccga tggagtccag
    23041 cgttatgtgc aggcgctctt tttcctgaaa caatgcctcg ttaagctgtt taacctcagt
    23101 catatccata ttaatgccga gcaggcgttc gacttcgcct tctttattca gtacccggtt
    23161 ggcgagggcg cggatatggc gaataccgtc ttttacggta atgcgaaatt ccagtttaaa
    23221 gggcgagcgt gattgcaacg aatcacgaat cactttttcg gcatgctggc gatcttccgg
    23281 cagcacgcac tcgtaccaca cctgccagtt cggtttgata tgtggaggaa tttcatacag
    23341 ctcgaacatc cgcttatccc agctaaaaat attcggcttc aactcccact cccagatgcc
    23401 aatcccgccc gcttcgttag ccagcgtgat gcgctccatc agttgctgat tcacctgttc
    23461 ggtgcgtttt agctcgttaa tgtcttcaat ctgagcgata aaatagagcg gcgtgccatc
    23521 cgtgtggcgc accagtgaga cggcaagcaa cgcccagaca acatcgccat tgcggttgta
    23581 gtagcgtttt tccattgaat aggtgtttat ttcaccgctt atcagctttt caacctgttg
    23641 gagatcttta ttgagatcct ccggccaggt cagttgctga aaggtgagtc cgcgcagctc
    23701 ttcctgactg tacccgagaa actggcagag cgctttgttg gtttgcagcc attgtccctc
    23761 ggtgcccact aacgccatac cgatagcgga atattccatg gcgttccgaa aacgggtttc
    23821 gctttcggaa atgtgtttgc gttccgcacg aaaggcatac atcaccatcg tcatgatgtt
    23881 ggcgggcagc aggatcagca aaaacggtag ccacggcata tggctcatca ggtatgtacg
    23941 cggcgtagca agcagggagg gatccgcggc catcatcagc gacaccatca tcaccgtggt
    24001 aaggaagatc aaaaaggctt ccattcgtgg caggcgcacg gcgctccaca tcaacagcac
    24061 aataatgaaa gtaaaaggcc acggcagata cagcatcgaa agccagctta acgtcagtgt
    24121 gatggctaac gtgagcagcg actcaaaaag caaccgtggg ttgcgatggc gcagcagata
    24181 gtgtggttta aataacaatc ccagcggcac cagtgccaga gcgccgatgg attctgacag
    24241 tacccatatc aaaaatgccc tgagaggatc gtctccgggc gtcagcagga caaccagaac
    24301 accccctagc agaggcggaa caatggcgct gccgaatgcc agacgcagcc agtcagccag
    24361 attttgcaag gggttatacc acggcagcaa tttacgtagc agcactgccc cgaccacggc
    24421 ttcaacaata ttgatggtcg tccaggtcat gttcagcgag ctggtggaaa aaagcaggat
    24481 ggatgcggcg atatttccca gcgagcagct cagcgcaatt cccggccaca tgcgcccggc
    24541 atggcgataa aacgccacca tcatgatgga cgtcgggaac cacagtgggg cgagttgggt
    24601 gccaaactgc gaaagctcaa gcgagaaaag ggtaaagata aacgagacta aacctaaact
    24661 gaccaggtga agcagcgggt ggggcagggc aattaataca tgctgtgatt gtttgctcat
    24721 taccgcttta tccgcaagac caggtcgcca gtattcgttt ctgtgtttat gctagtacaa
    24781 aagttttaca ttttatatgc gaattgctca taaagtgacg taaaggcgga taatttgcgc
    24841 aactgcgtat aacatttttt accttacata aaactgatca acgtaatttg ccaccgggaa
    24901 ttctttgtca acaattaact ctgagcgcgc ggcaacgcta ttcgactggt atcagacgga
    24961 tgaaatccct ataattgccg cgtttggcgc ttcgtcgccc ccttcctaac atccaggtta
    25021 atcaggtcgc taaatatatg actgatcagt ctcatcagtg cgtcattatc ggtatcgctg
    25081 gcgcatcggc ttccggcaag agtcttattg ccagtaccct ttatcgtgaa ttacgtgagc
    25141 aagtcggtga tgaacacatc ggcgtaattc ccgaagactg ctattacaaa gatcaaagcc
    25201 atctgtcgat ggaagaacgc gttaagacca actacgacca tcccagcgcg atggatcaca
    25261 gcctgttgct tgagcattta caagcgttga aacgcggctc ggcaattgac ctgccggttt
    25321 acagctatgt tgaacatacg cgtatgaaag aaacggtgac ggttgagccg aagaaggtca
    25381 tcattctcga aggcattttg ttgctgacgg atgcgcgttt gcgtgacgaa cttaacttct
    25441 ccattttcgt tgataccccg ctggatatct gcctgatgcg ccgcatcaag cgtgacgtta
    25501 acgagcgtgg gcgttcaatg gattccgtga tggcgcaata tcaaaaaacc gtgcgcccga
    25561 tgttcctgca attcattgag ccttctaaac aatatgcgga cattatcgtg ccgcgcggcg
    25621 ggaaaaaccg catcgcgatc gatatattga aagcgaaaat aagtcagttc tttgaataag
    25681 cttgataaat tgtgtaccgt tcagtgataa cctggtatgc ccttgacgta attggcgtta
    25741 agggagtgat gcgcgaaagg agaaaatgcc atgcgtctgt gtgaccgaga tattgaagcc
    25801 tggcttgatg aaggccgttt gtcgatcaac ccacgtccgc cagtggagcg tattaacggc
    25861 gcgacggtgg atgtacgcct gggcaataaa tttcgtacct tccgtggtca cacggcagcg
    25921 tttatcgatc tgagcggtcc caaagatgaa gtgagcgccg cgcttgaccg cgtgatgagc
    25981 gatgagatcg ttctcgacga gggcgaggcg ttctatcttc acccaggaga gctggcgctg
    26041 gcggtgacgc tggagtcggt gacgctgcca gccgatctgg tgggctggct ggacgggcgt
    26101 tcctctttgg cgcgtctggg gctgatggtg cacgtcaccg cgcaccgtat cgatccgggc
    26161 tggtctggtt gcattgtgct ggagttctac aactccggta agctgccgct ggcgctgcgt
    26221 ccgggcatgt taattggtgc gctgagcttt gagccgcttt ccggcccggc ggcgcgacct
    26281 tacaaccgcc gtgaagatgc gaaatatcgc aaccagcagg gcgcggtagc cagccgaatc
    26341 gataaagact aattcaggcc cattgaggat accatgagac gatttctgac gacgctgatg
    26401 atactcctgg tcgtgctggt ggccgggtta tctgcgttag tgttgctggt gaatccgaat
    26461 gatttccgcg actatatggt caagcaagtt gctgcacgta gcggttatca attgcagctc
    26521 gacgggccac tgcgttggca cgtctggccg cagcttagta tcctctccgg gcgaatgtct
    26581 ctcaccgccc agggcgcaag ccagccactg gttcgcgccg acaacatgcg tctggacgtg
    26641 gcgcttttac cactactgag tcatcaactg agcgttaagc aggtgatgct aaaaggggca
    26701 gtgatccaac tgacgccgca gacggaagcg gtgcgcagtg aagacgctcc ggttgcaccg
    26761 cgcgacaata ccttgccgga tctgtcagac gatcgcggat ggtcgtttga tatatccagt
    26821 cttaaggtgg cggacagcgt gctggtgttc cagcatgaag atgacgagca ggtgacaatc
    26881 cgcaatatcc gcctgcaaat ggaacaagat ccccaacatc gtggctcatt tgagttctcc
    26941 gggcgggtta atcgcgatca gcgcgatctc acgatatccc ttaacggtac ggtagatgct
    27001 tctgattatc cgcatgattt aacggcggct attgaacaaa ttaactggca gttgcagggt
    27061 gcagatttac caaaacaagg tattcagggg caggggagtt tccaggccca gtggcaggag
    27121 tcacataaac gcctttcatt taaccaaatc agcttgaccg ccaatgacag cacgctgagc
    27181 gggcaagcac aggtcacgct gacagagaaa cctgaatggc agctgagact gcaattcccg
    27241 caactgaatc ttgacaacct catcccgctt aatgaaacag cgaatggtga aaacggtgcc
    27301 gcgcagcagg ggcagagcca atcaacattg ccgcgcccgg tcatttcttc gcgtattgat
    27361 gaaccggcat atcagggact gcaaggcttt acggctgata ttttgttgca ggccagtaac
    27421 gtgcgctggc gcggaatgaa ttttacagat gttgccacgc aaatgaccaa caagtcgggt
    27481 ttgctggaaa ttactcaact gcagggcaag cttaacggtg gacaggtttc actgccgggc
    27541 acgctggacg cgacatcaat aaatccgcgg ataaacttcc agccacggct ggaaaacgtt
    27601 gagattggta ccattctgaa ggcgtttaac tatccgattt cgttgaccgg aaaaatgtca
    27661 ctggctggtg atttctccgg tgctgacata gatgccgacg cattccgcca caactggcaa
    27721 ggacaggcac atgtcgaaat gaccgacacg cgcatggaag ggatgaactt ccagcagatg
    27781 attcagcaag cggtagaacg taatggtggt gatgtgaagg ccgctgaaaa cttcgataac
    27841 gtaacgcgtc ttgaccgctt taccaccgat ttgacgttga aggatggcgt cgtgacgtta
    27901 aacgacatgc aaggtcaatc gcctgtgctg gcgctgacag gggaaggcat gttgaatctg
    27961 gcagatcaaa cctgcgacac ccagtttgat attcgggtcg tgggtggctg gaacggggaa
    28021 agcaaactga ttgatttcct caaagaaacg ccagtaccgc tgcgggttta tggcaactgg
    28081 cagcaactca attacagtct gcaagtggat cagttactgc gcaaacatct acaggacgaa
    28141 gcgaaacgtc gcctgaatga ctgggccgag cggaataaag attcccgcaa tggcaaagat
    28201 gtgaagaagt tgctggagaa gatgtaggtt accaggtgac ctgtcctgta ggcctgataa
    28261 gacgcgtaaa gcgtcgcatc aggcgttcgc ggcggcacca ttatattctg aatatgtaag
    28321 atcgaaggct gttccgttca cttgatgttc ctcactacta tggaacgctc aagccggtga
    28381 acgtgccagg gtggctcaat cgccaccacc ctggcaaccc gggctcccgg cggtaaatcg
    28441 ccgctggcgc ggttccctca tcttattcct ttcggctatc gggtaaaatg accgatacca
    28501 tcacacctcg tactccatct cgccatcctt acgcagtgga ataatctgca ccttctgcac
    28561 gcgatggctt tccacctgca acgttttaag caaataatcc cccacctgaa cttcttcgcc
    28621 aggttttgga atacgttgca aatactccat caacagcccg gcaatggtgt gatattcacg
    28681 tttttcatcc agcggcagcg gcacatattg caccagatct tccagcggca tatgaccatt
    28741 cgccgtccag gaaccgtccg cattcttctg aatatcatgg cgggcgtcga tctcttccac
    28801 ttcgttcggt aagttaccgg caatggtttc agtgacgtca cttaatgtca caatcccttc
    28861 caccgagcca aactcatcca ccacaaaagc aaagtgcgtg cgggcattac ggaactgctc
    28921 cagggcaggt aacaacggca aggtttccgg gaacaccagc ggctggcgaa tcaacacccg
    28981 caggttgagc ggttcgccgc gcagtgactg ttgtagcagg tcgataacgt gaacaacacc
    29041 gagcaaatct tctgcatcat caccgtcggt aaccaccagc cgcgtatgct gatttcgctc
    29101 cagtaactgg cggatctcgt cttccggcgc gttgagatcg atatgctcaa tatcgtggcg
    29161 cgacgtcata atgctgctga cggtacgctg gttaagatta agtacccgct caatcatccg
    29221 ccgttcctgc ggattaaaga tctgctggtt accatgatcc accagcatgg acgcggtttc
    29281 ggcgtccagt tccgcatctt ctttttgccc gctaagcagg cgcattaccg cttcggtagt
    29341 ccgctggcgc agcgtctggt tagcggaaag aaaacgtcgc cggttaaaga tagccaactg
    29401 attgagcgcc tcgatcatca ccgagaaacc aatggcagcg tacaggtagc ctttcggaat
    29461 gacgaagccg aaaccttctg ccaccaggct aaagccaatc atcaacagga agcttaagca
    29521 gagaataacg atcgtcgggt gactgttaac aaattgcgtt aacggcttgc tggccatcaa
    29581 catcaggctg atagcgataa ccacggcggc catcatgacc agtaaatggt cgaccatccc
    29641 gacggcggta atcactgagt cgagtgagaa gatggcgtcc agtaccacaa tttgtgtcac
    29701 cactccccag aacttcgcac ctttgcgttg tgtgggatta ttgctgtctt tcccttccag
    29761 ccgttcgttc agctccatcg tggctttgaa caacaggaag aaaccaccaa acagcatgat
    29821 taagtcgcgg gcgctaaagg taaacgagcg gaagctgaac agcggttgag tcagggtgac
    29881 cagccaggag attgacgcca gcagtaacag gcgcattaac atcgccagca gcagcccggt
    29941 aacccgtgcg cggtcgcgct gcttcggcgg tagtttttcg gcgaggatgg caataaagac
    30001 caggttatca atgccgagga ccagttcgat cacaatcagc gtgattaacc cggcccagat
    30061 agacggatcg gcaatccatt ccatagtaag tacaaaacct attcgttgta tgacaaatgt
    30121 cacaattcga tcatggataa atcggggcaa aaatgcaatg ttgcagcact atttatctca
    30181 tattatttga gggtaataac ctcacattat ccctgaatta aaagtggtaa taataaagca
    30241 ttatctaaga aatatcgcat gtggagacgc aatattaaat attgtcaacc taaagaaact
    30301 cctaaaaacc atattgaatg acacttaata taattcttaa aaatagccaa ttgccgaatt
    30361 gttaccttgc ctgctattcc gttagctgta acacttcctc ctgcattatt ggagagccaa
    30421 tattcaatta ccataatgtc accttatatt cggaagttaa tttccgcaat aaccctttct
    30481 catcaacgat tgtatgcgga gagggtttga aaaattaagc taaatcagtg tattggtagc
    30541 taaaaagcca ggggcggtag cgtgtctgga tgcctgaaag accagtctga attattctgt
    30601 caatagcctg cggatgaaat caattttttt aggactgatg ccagttaaat ttttattcaa
    30661 ttaactgcaa ggtaaatgtg catggcgaca ttgctattaa tagtgcacag gataattact
    30721 ctgccaaagt gataaataaa caatgatgaa atccaaaatg aaattgatgc cattattggt
    30781 gtcagtaacc ttgataagcg gttgcacagt acttccgggc agcaatatgt cgacgatggg
    30841 caaagacgtc atcaaacagc aggacgctga tttcgatctc gacaaaatgg tgaatgttta
    30901 tccgctgacc ccgcgcctga ttgaccaatt acgcccacgc ccgaatgtag cgcgccccaa
    30961 tatgacgctg gaaagcgaga tcgcgaatta ccagtatcgc gtcgggccgg gcgacgttct
    31021 gaatgtcacc gtctgggatc acccggaact caccacgcca gccggtcagt accgcagctc
    31081 cagcgacacc ggcaactggg tacagcctga cggcactatg ttttacccgt atatcggcaa
    31141 ggtccacgta gtcgggaaaa cgctcgctga aatccgcagt gatattaccg ggcgcttagc
    31201 gacgtacatc gctgacccgc aggtggatgt taatatcgcc gccttccgct cgcaaaaggc
    31261 ctatatctca ggtcaggtga ataaatccgg tcaacaggcg atcaccaacg taccactgac
    31321 cattctcgac gccatcaacg ccgcaggtgg cctgaccgac accgctgact ggcgcaacgt
    31381 ggtgctaaca cacaatggtc gtgaagaacg catttctttg caggcgctga tgcaaaacgg
    31441 cgacctcaat cagaaccgac tgctttaccc cggcgatatt ctctacgtgc cgcgtaatga
    31501 tgatctgaaa gtgtttgtga tgggtgaagt gaagaaacag agcaccctga aaatggactt
    31561 tagcggcatg accctgactg aagccctggg caatgccgaa ggtatcgaca tgaccacctc
    31621 caacgccagc ggcatctttg tcattcgtcc gctgaaaggc gagggcgggc gtaacggcaa
    31681 gattgccaat atctaccagc tggatatgtc cgatgcgacg tcgctggtga tggcgacaga
    31741 attccgcctg caaccttatg acgtggtgta tgtcaccacc gccccggttt cccgctggaa
    31801 ccgtctgatc aatcagttgc tgccaactat tagcggtgtc cgttacatga cggatacagc
    31861 cagcgacatt cataactggt aatcgtcatg tttaacaaca tcttagttgt ctgtgtcggc
    31921 aatatttgcc gttccccgac ggcggaacgc ttactgcaac gttatcaccc ggagctgaaa
    31981 gtggagtccg ctggactcgg cgcgctggtc ggtaagggcg ctgatcctac cgctatcagc
    32041 gtcgccgcag aacatcaact gtctctggaa ggtcactgtg cacggcaaat cagccgccgc
    32101 ctgtgtcgca actatgacct gattttgacc atggaaaagc gccatatcga acgcttatgc
    32161 gagatggcac ctgagatgcg tggcaaagtg atgctgtttg gtcactggga taacgaatgt
    32221 gaaatccccg atccgtatcg caaaagccgg gaaacgtttg cagcggtgta cacattactt
    32281 gaacggtctg cccgccagtg ggcgcaggca ttgaacgcag agcaggtata agaatgacag
    32341 aaaaagtaaa acaacatgcc gctccggtaa cgggcagtga tgaaatcgat attggtcgcc
    32401 tggtcggcac cgtcattgaa gcgcgctggt gggtgattgg catcaccgct gtatttgccc
    32461 tctgtgctgt ggtttacact ttcttcgcca cgccgattta tagtgccgac gcactggtac
    32521 aaatcgaaca aaacagcggc aattcgttag tgcaggacat tggctcggca ttagccaaca
    32581 aaccgcctgc atcggacgcc gagatccagt tgattcgttc gcgtctggtg cttggtaaaa
    32641 cggtggacga tctagacctc gatattgcgg tgagcaaaaa cacgttcccg attttcggtg
    32701 cgggttggga tcgcctgatg ggacgtcaga acgagacggt gaaagtgacc acctttaacc
    32761 gcccgaaaga gatggcggac caggtgttta cgcttaatgt gctggatgac aaaaactaca
    32821 ctctgagcag tgatggcggc tttagcgccc gtgggcaagc gggccagatg ctgaaaaaag
    32881 aaggcgtcac gctgatggtt gaagccattc acgcccgccc gggcagtgag tttaccgtca
    32941 ccaaatactc cacgttgggg atgatcaacc aactgcaaaa cagcctgacg gtaacggaga
    33001 acggcaaaga cgcaggcgta ctgagcctga cttataccgg tgaagatcgc gaacagatcc
    33061 gcgacattct taacagcatc gcccgtaact atcaggaaca aaatattgag cgcaaatcgg
    33121 cggaagcgtc gaaaagcctc gctttcctcg cgcaacagtt accggaagta cgtagccgcc
    33181 tcgatgttgc cgaaaacaaa ctgaatgcct tccgtcagga taaagattct gttgatctgc
    33241 cgctggaagc gaaagcggtg ctcgattcga tggtgaacat cgacgcgcag ttgaacgaac
    33301 tgacctttaa agaggcggaa atctccaagc tgtacaccaa agttcacccc gcgtaccgca
    33361 cgctgctgga gaaacgtcag gcgctggaag acgaaaaagc caaacttaat ggtcgcgtaa
    33421 cggcgatgcc gaaaacccag caggaaattg tccgtctgac ccgcgatgtc gagtctggtc
    33481 agcaggtcta tatgcaactg ctgaataaag agcaggagct gaaaatcacc gaagccagca
    33541 ccgtcggcga tgtgcgcatt gttgacccgg caatcaccca gccaggtgta ctgaaaccga
    33601 agaaagggct gattatcctt ggggcgatta ttcttggcct gatgctctct atcgtgggtg
    33661 tgctgctgcg ctcgttgttt aatcgcggca tcgaaagccc gcaggtgctg gaagaacacg
    33721 gtatcagcgt ctatgccagc atcccgttgt cggaatggca gaaagcgcgc gatagcgtca
    33781 aaaccatcaa agggattaaa cgctataaac agagccagct actggcggtg gggaatccaa
    33841 ccgatctggc gattgaagcc atccgcagtc tgcgtaccag tctgcacttc gcgatgatgc
    33901 aggcgcagaa caatgtgttg atgatgaccg gggttagccc gtcaatcggt aaaacctttg
    33961 tctgcgccaa cctggcggcg gtgatcagcc agaccaataa acgcgtgttg ttgatcgact
    34021 gcgatatgcg caaaggctac acccacgagc tgttgggcac caataacgtc aatggcctgt
    34081 cggaaattct gattggtcag ggcgatatta ctaccgctgc taaaccgacc tctattgcca
    34141 aatttgacct gatcccgcgc ggtcaggtac cgccaaatcc ttctgaactg ttgatgagcg
    34201 aacgctttgc cgaactggtg aactgggcga gtaaaaacta cgacctggtg ttgattgata
    34261 cgccgccgat tctggcagtg accgatgcgg caattgttgg tcgtcatgtt ggaaccacgt
    34321 taatggtggc gcgttatgcg gtcaacacat tgaaagaagt ggaaaccagt ctgagccgct
    34381 ttgagcaaaa cggtattccg gtgaaagggg tgattctgaa ctccatcttc cgccgcgcca
    34441 gcgcgtatca ggattatggc tattacgaat acgaatataa gtcggatgcg aaataacgac
    34501 gcctgcattc gcaccgcccc gtaggccgga taaggcgctc acgccgcatc cggcaagcaa
    34561 accagctcat aagccgggag aacaacctat gaaagataat ccgctgatct caatctatat
    34621 gccaacctgg aaccgccaac aactggcgat tcgggcgata aaatcggtcc tgcgccagga
    34681 ctacagcaac tgggagatga tcatcgtgga tgattgctcc acctcctggg aacaactgca
    34741 acagtacgtc accgccctca acgatccgcg tatcacttac atccacaacg atattaacag
    34801 cggggcgtgc gcggtacgta accaggctat tatgctggcg caaggggaat acattaccgg
    34861 gatcgatgac gatgacgaat ggacacccaa ccgcctgagc gtcttcctcg cccataaaca
    34921 gcaactggtc actcacgcct ttttgtacgc taacgactac gtatgccagg gagaagttta
    34981 ttcccaacca gcaagcttac cactgtatcc caaatcgccg tactcacgcc gcctgttcta
    35041 taaacgcaat atcattggca atcaggtttt tacctgggcg tggcgtttca aagagtgctt
    35101 gttcgatacc gaactgaaag cagcacagga ttacgacatc ttcctgcgga tggtggtgga
    35161 gtatggcgaa ccgtggaaag tggaagaggc gacgcagatc ctgcatatca atcacggtga
    35221 aatgcagatc acctcgtcac cgaaaaaatt ctccggatac ttccactttt accgcaagca
    35281 caaagacaaa ttcgaccgcg ccagcaaaaa atatcagctg tttaccctct accagatccg
    35341 caataaacgt atgacctggc gcactttgct gacgctgctg tcggtgcgta acggcaaacg
    35401 tctggctgat ggtattcggg ggcgctaaag atgctggaag atctgcgcgc caacagctgg
    35461 agtttacgtc cgtgctgcat ggttctagcc tatcgcgtcg ctcatttttg ttcggtgtgg
    35521 cgcaaaaaga acgtcctcaa caatctgtgg gcggcccctc tgctggtgct gtatcgcatt
    35581 atcaccgaat gctttttcgg ttatgaaatc caggctgccg cgaccattgg ccgccgcttt
    35641 actatccatc acggttacgc cgtagtgatc aataaaaacg tggtggcggg ggatgatttc
    35701 accattcgcc acggcgtcac tatcggcaat cgtggtgccg ataacatggc atgtccacat
    35761 attggcaacg gcgtcgaact cggtgccaac gttattattc ttggtgatat cacgcttggt
    35821 aacaacgtca ccgtgggtgc gggcagcgtg gtgctcgact ctgtcccgga caacgcgctg
    35881 gtggtgggag aaaaagcgcg agtgaaggta attaaatgaa tattttgcaa tttaatgtgc
    35941 gactggcgga aggcggggca gcaggtgtgg cgttagatct ccaccagcgt gcgctgcaac
    36001 aggggctagc gtcacatttt gtctacggtt acggcaaagg cggcaaagag agcgtcagcc
    36061 atcaaaacta tccgcaggtc atcaaacata cgccgcggat gactgcaatg gcgaatattg
    36121 tcctgtttcg tctgtttaat cgcgatctgt ttggcaattt caatgagtta tatcgcacca
    36181 ttactcgtac accgggtccg gtggtcctgc attttcatgt gctgcacagc tactggctaa
    36241 atcttaagag cgtggtgcgc ttttgcgaaa aggtgaaaaa ccataaaccg gacgtcactc
    36301 tggtctggac gctgcacgac cactggagcg ttaccggacg ctgcgccttt accgacggtt
    36361 gtgaaggctg gaaaacgggc tgccagaaat gcccgacctt aaataattat ccgccggtga
    36421 agattgatcg cgcacaccaa ctggtggcgg gcaaacgcca gttattccgt gagatgctgg
    36481 cgctgggctg tcagtttatt tcccccagcc agcatgtggc tgacgctttc aatagcctgt
    36541 acggtccagg gcgttgccgg attatcaata acggtattga tatggcaacc gaagcgattc
    36601 tggcggatct gcctccggtg cgcgaaaccc agggtaagcc gaaaatcgcg gtggtggcgc
    36661 atgacctgcg ttacgacggc aaaactaacc agcaactggt gcgtgagatg atggcgctgg
    36721 gcgacaaaat cgaactgcat acctttggta agttctcgcc gttcaccgct ggtaacgtgg
    36781 ttaatcacgg ctttgaaacc gacaaacgta agctgatgag cgcgctcaat cagatggatg
    36841 cgctggtgtt cagttctcgc gtcgataact acccgctgat tttgtgtgag gcgctatcga
    36901 ttggcgtgcc ggtgatcgcc acccatagcg atgcggcgcg ggaagtgctg caaaaatccg
    36961 gtggtaaaac cgtcagtgaa gaagaggtgc tgcaactggt gcagttaagc aaaccggaaa
    37021 tcgcgcaggc gatatttggt accacgctgg ctgagttcag ccaacgcagc cgcgccgcct
    37081 acagtggaca acagatgctg gaggagtatg tcaacttcta tcagaatctg tagctacctg
    37141 ctgctgccgc tgatctacct gctggttaac gtcaaaatcg cccagcttgg tgaaagtttc
    37201 cccatcacta tcgtcacttt tttacctgtc ttgttactgc tgtttttaga acgcatcagc
    37261 gttaaaaaat tgatgattgc cttagggatt ggtgcgggac tcacggcgtt caactatctg
    37321 tttggtcagt cgttggatgc cagtaaatac gtcacttcaa ccatgctgtt tgtctatatt
    37381 gtgatcatta ttggcatggt gtggagtatt cgctttaaaa cgatttcgcc acacaaccat
    37441 cgcaagatat tacgtttctt ttatctggtg gtcgggctgg tggtggcgct ggcggcggtg
    37501 gagatggcgc aaattatcct caccggcggc agcagtatta tggagtcgat ttcgaaatat
    37561 ctgatttaca gcaacagcta tgtgctgaat ttcatcaaat tcggcggcaa gcgcacgaca
    37621 gcactttatt tcgaaccggc atttttcgct ctggcattaa tctcaatttg gctcagcatc
    37681 aaacagtttg gtatcaaaac gcctaaaacc gatgctatga ttctcgcagg gataatatta
    37741 tccggatcgt tttcaggggt tatgaccttt atcctgtttt atttgctgga gtgggcattc
    37801 caatatctga ataaagaggc gattaagaaa aagttaccgt tagcattgat ttctctggct
    37861 gtattcctgg ttggtgtggt aatcgcgttt ccttatattt ccacccgtct gggcgattta
    37921 ggtacggaag gatcgtcatc gtattatcgt attgtcggtc cgctggtgat ggtcggttat
    37981 tctttgaccc atattgacgg tgtagtcaga tttggctcac tttatgaata tgtcgcatca
    38041 ttcggaatat ttaacggtgc ggatgtcgga aaaaccatag acaatggttt gtatctgctg
    38101 attatttatt tttcctggtt cgcggtgttt ttatcgctgt ggtacatggg gaaagtgata
    38161 aaaatgatga tcaacgcttt tggtgataac cgcaattttc gcgtgcaatt atatcttttt
    38221 actccggtat cgctgttttt taccggttcg atatttagcc cggaatatgc atttttaatc
    38281 gtctgtccgt ttattttgcg aaaagcgtta aatattacga ggtaagaata agaacatgtt
    38341 gcttagcata atcactgtcg cgtttcgtaa cctcgaaggg atagtcaaaa cacatgcctc
    38401 gcaggcgcat ctggcgcagg cggaagatat cagcttcgaa tggattgttg tcgatggcgg
    38461 ttccaatgac ggcacccgtg agtatctgga aaatctcaat ggtatctata acctacgctt
    38521 tgtcagcgag ccagataacg gtatctatga cgccatgaac aaaggcatta cgatggcaca
    38581 aggcaagttc gcgttgtttc tcaattcggg cgatattttt catcagaatg ccgcaaattt
    38641 tgtccgtaag ttaaaaatgc aaaaagataa cgtgatgatc accggcgatg cgctgctgga
    38701 ttttggcgac gggcataaaa ttaaacgtag cgccaaaccg ggctggtata tttatcacag
    38761 cctgcccgcc agtcatcagg cgatattttt cccggtatcc ggcttgaaga aatggcgtta
    38821 tgacctggaa tataaagttt cttccgacta cgcgctggca gccaaaatgt ataaagccgg
    38881 ttatgcattt aaaaaactca atggcctggt gtctgaattt tccatgggtg gggtatctac
    38941 caccaataat atggaattgt gtgctgacgc gaaaaaagtc caacgacaaa tattacatgt
    39001 acctggcttt tgggctgaat tatcctggca tttacgccaa cgtactacct caaagacgaa
    39061 agccttatat aacaaagtct gaatataagg gaaaacgatg caggatttaa gtggattctc
    39121 ggtgccgaaa gggttccggg gcggcaacgc tattaaagtg caattatggt gggcagtaca
    39181 ggcaacaata tttgcctggt cgccacaagt attgtatcgc tggcgggctt ttttattacg
    39241 tttattcgga gcaaaaatag gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta
    39301 tccgtggaaa ttaaccttag gtgattacgc gtgggtcggc gatgacgtca atttatatac
    39361 cctcggtgaa ataaccattg gcgcacattc ggtgatatcg caaaaaagtt atttatgcac
    39421 cggtagccac gaccatgcaa gtcaacattt caccattaac gccacgccta ttgtgattgg
    39481 cgagaaatgc tggctggcaa ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg
    39541 caccgtcgtg ggtgcacgaa gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg
    39601 ggggaatccc gcagtggtga tacgcgaacg cgttgaaact gaataaattc aaaaatacag
    39661 aggaataaga catgtcaaaa gtcgctctca tcaccggtgt aaccggacaa gacggttctt
    39721 acctggcaga gtttctgctg gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat
    39781 cgtcattcaa caccgagcgc gtggatcaca tttatcagga tccgcacacc tgcaacccga
    39841 aattccatct gcattatggc gacctgagtg atacctctaa cctgacgcgc attttgcgtg
    39901 aagtacagcc ggatgaagtg tacaacctgg gcgcaatgag ccacgttgcg gtctcttttg
    39961 agtcaccaga atataccgct gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga
    40021 tccgcttcct cggtctggaa aagaaaactc gtttctatca ggcttccacc tctgaactgt
    40081 acggtctggt gcaggaaatt ccgcagaaag aaaccacgcc gttctacccg cgatctccgt
    40141 atgcggtcgc caaactgtac gcctactgga tcaccgttaa ctaccgcgaa tcctacggca
    40201 tgtacgcctg taacggtatt ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg
    40261 ttacccgcaa aattacccgc gcaatcgcca acatcgccca ggggctggag tcgtgcctgt
    40321 acctcggcaa tatggactcc ctgcgtgact ggggccatgc caaagactac gtaaaaatgc
    40381 agtggatgat gctgcaacag gaacaaccgg aagattttgt tatcgcgacc ggcgttcagt
    40441 actccgtacg tcagttcgtg gaaatggcgg cagcacagtt gggcatcaaa ctgcgctttg
    40501 aaggcacggg tgttgaagag aagggcattg tggtttccgt caccgggcat gacgcgccgg
    40561 gcgttaaacc gggtgatgtg attatcgctg ttgacccgcg ttacttccgt ccggctgaag
    40621 ttgaaacgct gctcggcgac ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa
    40681 tcaccctcag agagatggtg tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac
    40741 actctctgct gaaatctcac ggctacgacg tggcgatcgc gctggagtca taagcatgag
    40801 taaacaacga gtttttattg ctggtcatcg cgggatggtc ggttccgcca tcaagcgaca
    40861 gctcgaacag cgcggtgatg tggagctggt attacgcacc cgcgatgaac tgaacctgct
    40921 ggacagccgc gcggtgcatg atttctttgc cagcgaaagc attgaccagg tctatctggc
    40981 ggcggcgaaa gtgggcggca ttgttgccaa caacacgtat ccggcggatt tcatctacca
    41041 gaacatgatg attgagagca acatcattca cgccgcgcat cagaacgacg tgaacaaact
    41101 gctgtttctc ggatcgtcct gtatctaccc gaaactggca aaacagccga tggcagaaag
    41161 cgagttattg cagggtacgc tggagccgac caacgagcct tatgccattg ccaaaatcgc
    41221 cgggatcaaa ttgtgcgaat cttacaaccg ccagtacgga cgcgattacc gctcggtcat
    41281 gccaaccaac ctgtacgggc cacacgacaa cttccatccg agtaactcgc atgtgatccc
    41341 agcattgctg cgtcgcttcc acgaggcgac ggcacagaat gcaccggatg tggtggtgtg
    41401 gggcagcggt acaccgatgc gtgaatttct gcacgtcgat gatatggcgg cggcgagcat
    41461 tcatgtcatg gagctggcgc atgaagtctg gctggagaac acccagccga tgctgtcgca
    41521 cattaacgtt ggtacgggcg ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa
    41581 agtggtggat tacaaaggtc gggtggtttt tgatgccagc aagccggatg gtacgccgcg
    41641 caaactgctg gatgtgacgc gcctgcatca gcttggctgg tatcacgaaa tctcactgga
    41701 agcggggctt gccagcactt accagtggtt ccttgagaat cgagaccgct ttcgggggta
    41761 atgatgtttt tacgtcagga agactttgcc acggtagtgc gctccacacc gcttgtctct
    41821 ctcgacttta ttgccgagaa cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc
    41881 ccggcgcagg gttactggtt tgtgccggga gggcgcgtgc agaaagacga aacgctggaa
    41941 gccgcatttg agcggctgac gatggcggaa ctggggctgc gtctgccgat aacagcaggc
    42001 cagttttacg gtgtctggca gcacttttat gacgataact tctctggcac ggatttcacc
    42061 actcactatg tggtgctcgg ttttcgcttc agagtatcgg aagaagagct gttactgccg
    42121 gatgagcagc atgacgatta ccgctggctg acgccggatg cactgctcgc cagtgacaat
    42181 gttcacgcta acagccgcgc ctattttctc gctgagaagc gtgccggagt acccggatta
    42241 tgaaaatact ggtctacggc attaactact cgccggagtt aaccggcatc ggcaaataca
    42301 ccggcgagat ggtggaatgg ctggcggcac aaggtcatga ggtgcgggtc attaccgcac
    42361 cgccttacta cccgcagtgg caggtgggtg agaactattc cgcctggcgg tacaaacgag
    42421 aagagggggc cgccacggtg tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc
    42481 tgaaacgcct gttgcatctc agcagttttg ccgtcagcag tttctttcca ctgatggcgc
    42541 aacgtcgctg gaagccggat cgcattatcg gcgtagtgcc aacgctgttt tgcacgccgg
    42601 gaatgcgcct gctggcgaag ctctctggtg cgcgtaccgt gctgcatatt caggattacg
    42661 aagtggacgc catgctgggg ctgggccttg ccggaaaagg caaaggcggc aaagtggcac
    42721 agctggcaac ggcgttcgaa cgtagcggac tgcataacgt cgataacgtt tccacgattt
    42781 cgcgttcgat gatgaataaa gccatcgaaa aaggcgtggc ggcggataac gtcatcttct
    42841 tccccaactg gtcggaaatt gcccgttttc aacatgttgc agacgccgat gttgatgccc
    42901 ttcgtaacca gcttggcctg ccggataaca aaaaaatcat tctttactcc ggcaatattg
    42961 gtgaaaagca ggggctggaa aacgttattg aagctgccga tcgtctgcgc gatgaaccgc
    43021 tgatttttgc cattgtcggg cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc
    43081 agcgtggact gcgcaacatg caatttttcc cgctgcaatc gtatgacgct ttacccgcac
    43141 tgctgaagat gggcgattgc catctggtgg tgcaaaaacg cggcgcggca gatgccgtat
    43201 tgccgtcgaa actgaccaat attctggcgg taggcggtaa cgcggtgatt accgcagagg
    43261 cccacacaga actggggcag ctttgcgaaa cctttccggg cattgcggtt tgcgttgaac
    43321 cggaatcggt tgaggcgctg gtggcgggga ttcgtcaggc gctcctgctg cccaaacaca
    43381 acacggtggc acgtgaatat gccgaacgca cgctcgataa agagaacgtg ttacgtcaat
    43441 ttataaatga tattcgggga taattatggc gcagtcgaaa ctctatccag ttgtgatggc
    43501 aggtggctcc ggtagccgct tatggccgct ttcccgcgta ctttatccca agcagttttt
    43561 atgcctgaaa ggcgatctca ccatgctgca aaccaccatc tgccgcctga acggcgtgga
    43621 gtgcgaaagc ccggtggtga tttgcaatga gcagcaccgc tttattgtcg cggaacagct
    43681 gcgtcaactg aacaaactta ccgagaacat tattctcgaa cctgcagggc gtaacactgc
    43741 accggccatt gcgctggcgg cgctggcggc aaaacgtcat agcccggaga acgacccgtt
    43801 aatgctggtg ctggcggcgg atcatgtaat tgccgatgaa gacgcgttcc gtgccgccgt
    43861 gcgtaatgcc atgccatatg ccgaagcggg caagctggtg acctttggca ttgtgccgga
    43921 tctaccagaa accggttatg gctatattcg tcgcggtgaa gtgtctgcgg gtgagcagga
    43981 tacggtggcc tttgaagtgg cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc
    44041 ctatgtggca agcggcgaat attactggaa cagcggcatg ttcctgttcc gcgccggacg
    44101 ctatctcgaa gaactgaaaa aatatcgccc ggatattctc gatgcctgtg aaaaagcgat
    44161 gagcgccgtc gatccggatc ttgattttat tcgtgtggat gaagaagcgt ttctcgcctg
    44221 cccggaagag tcggtggatt acgcggtcat ggaacgtacg gcagatgctg ttgtggtgcc
    44281 gatggatgcg ggctggagcg atgttggttc ctggtcttca ttatgggaga tcagcgccca
    44341 caccgccgag ggcaacgttt gccacggcga tgtgattaat cacaaaactg aaaacagcta
    44401 tgtgtacgcc gaatctggcc tggtcaccac cgtcggggtg aaagatctgg tagtggtgca
    44461 gaccaaagat gcggtgctga ttgccgaccg taacgcggtg caggatgtga aaaaagtggt
    44521 cgagcagatc aaagccgatg gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc
    44581 gtggggcaaa tatgactcta tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt
    44641 gaaaccgggc gagggcttgt cggtacagat gcaccatcac cgcgcggaac actgggtagt
    44701 ggtcgcggga acggcaaaag tcaccattga tggtgatatc aaactgcttg gtgaaaacga
    44761 gtccatttat attccgctgg gggcgacgca ctgcctggaa aatccgggga aaattccgct
    44821 cgatttaatt gaagtgcgct ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc
    44881 ggatcgctac ggacgggtgt aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa
    44941 cgccttatcc gacctacggt tcgggtttgt agtcctgata agacgcggca gcgtcgcatc
    45001 aggcaatgaa tgcgaaaccg agatgtaaat aacgacaaaa acaaaattgg ccgtttcggt
    45061 cagggccaac tattgcctga aaaagggtaa cgacatgaaa aaattaacct gctttaaagc
    45121 ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatatcgcct ggcgcattgg
    45181 tcgcgcctat ggcgaatttc tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct
    45241 caccagcgaa accttaaaac tggcgctggc aaaaggttta caggatgcgg gcgttgacgt
    45301 gctggatatc ggtatgtccg gcaccgaaga gatctatttc gccacgttcc atctcggcgt
    45361 ggatggcggc attgaagtca ccgccagcca taatccgatg gattataacg gcatgaagct
    45421 ggtgcgcgaa ggggctcgcc cgatcagcgg tgataccgga ctgcgcgatg tccagcgtct
    45481 ggctgaagcc aacgactttc ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat
    45541 caacctgcgt gacgcttacg ttgatcacct gttcggttat atcaatgtca aaaatctcac
    45601 gccgctcaag ctggtgatca actccgggaa tggcgcagcg ggtccggtgg tggacgccat
    45661 tgaagcccgc tttaaagccc tcggcgcgcc cgtggaatta atcaaagtac acaacacgcc
    45721 ggacggcaat ttccccaacg gtattcctaa cccgctgcta ccggaatgtc gcgacgacac
    45781 ccgcaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgatg gcgattttga
    45841 ccgctgtttc ctgtttgacg aaaaagggca gtttatcgag ggctattaca ttgtcggcct
    45901 gctggcagaa gcattcctcg aaaaaaatcc cggcgcgaag atcatccacg atccacgtct
    45961 ctcctggaac accgttgatg tggtgactgc cgcaggtggc acgccggtaa tgtcgaaaac
    46021 cggacacgcc tttattaaag aacgtatgcg caaggaggac gccatctacg gtggcgaaat
    46081 gagcgctcac cattacttcc gtgatttcgc ttactgcgac agcggcatga tcccgtggct
    46141 actggtagcc gaactggtgt gcctgaaaga gaaaacgctg ggcgaactgg tacgcgaccg
    46201 gatggtggcg tttccggcaa gcggtgagat caacagcaaa ctggcgcaac ccgctgaggc
    46261 gattaaccgc gtggaacagc attttagccg tgaggcgctg gcggtggatc gtaccgatgg
    46321 catcagtatg acctttgccg actggcgttt taacctgcgc tcctccaaca ccgaaccggt
    46381 ggtgcggttg aatgtggaat cgcgcggtga tgtgccgctg atggaagcgc gaacgcgaac
    46441 tctgctgacg ttgctgaacg agtaatgtcg gatcttccct tatcccactg cgggtaaggg
    46501 gctaataaca ggaacaacga tgacaaatct aaaaaagcgc gagcgagcga aaaccaatgc
    46561 atcgttaatc tctatggtgc aacgcttttc agatatcacc atcatgtttg ccggactatg
    46621 gctggtttgc gaagtgagcg gcctgtcatt cctctacatg cacctgttgg tggcactgat
    46681 tacgctggtg gtgttccaga tgctgggcgg catcaccgat ttttatcgct catggcgcgg
    46741 tgttcgggca gcgacagaat ttgctctgtt gctacaaaac tggacgttaa gcgtgatttt
    46801 cagcgccgga ctggtggcgt tcaacaatga tttcgacacg caactgaaaa tctggctggc
    46861 gtggtatggg ctgaccagca tcggactggt ggtttgccgt tcatgtattc gcattggggc
    46921 gggctggctg cgtaatcatg gctataacaa gcgcatggtc gcggtggcgg gggatttagc
    46981 cgccggacaa atgctgatgg agagcttccg taatcagccg tggttagggt ttgaagtagt
    47041 gggcgtatac cacgacccaa aaccgggcgg cgtttctaac gactgggcgg gtaatctgca
    47101 acagctggtc gaggacgcga aagcgggcaa gattcataac gtctatatcg cgatgcaaat
    47161 gtgcgacggc gcacgagtga aaaaactggt ccatcaactg gcggatacca cctgttcggt
    47221 gctgctgatc cccgatgtct ttaccttcaa tattctccat tcacgtctcg aagagatgaa
    47281 cggcgttccg gtggtgccgc tgtatgacac gccactttcc ggggttaacc gcctgctcaa
    47341 acgtgcggaa gacattgtgc tggcgacgct tatcctgctg ctgatctccc cggtgttgtg
    47401 ctgtattgcg ctggcggtga aactcagttc accagggccg gttattttcc gccagactcg
    47461 ctacggcatg gatggcaagc cgatcaaagt gtggaagttc cgttcgatga aagtgatgga
    47521 gaacgacaaa gtggtgaccc aggcgacgca gaacgatccg cgcgtcacca aagtggggaa
    47581 ctttctgcgc cgcacctcgc tggatgaatt gccgcagttt atcaatgtgc tgaccggggg
    47641 gatgtcgatt gtcggtccac gtccacacgc ggtggcgcat aacgaacagt atcgacagct
    47701 cattgaaggc tacatgctgc gacataaggt gaaaccgggc attaccggct gggcgcagat
    47761 taatggctgg cgcggcgaaa ccgacacgct ggagaaaatg gaaaaacgcg tcgagttcga
    47821 ccttgagtac atccgcgaat ggagcgtctg gttcgatatc aaaatcgttt tcctgacggt
    47881 attcaaaggt ttcgttaaca aagcggcata ttgatatgag cttacgtgaa aaaaccatca
    47941 gcggcgcgaa gtggtcggcg attgccacgg tgatcatcat cggcctcggg ctggtgcaga
    48001 tgaccgtgct ggcgcggatt atcgacaacc accagttcgg cctgctgact gtgtcgctgg
    48061 tgattatcgc gctggcagat acgctttctg acttcggtat cgctaactcg attattcagc
    48121 gaaaagaaat cagccacctt gaactcacca cgctgtactg gctgaacgtc gggctggggc
    48181 ttgtggtgtg cgtggcggtg tttttgttga gtgatgccat tggtgacgtg ctgaataacc
    48241 cggacctggc accgttgatt aaaacattgt cgctggcgtt tgtggtgatc ccccacgggc
    48301 aacagttccg cgcgttgatg caaaaagagc tggagttcaa caaaatcggc atgatcgaaa
    48361 ccagcgcggt gctggcgggc ttcactttta cggtggttag cgcccatttc tggccgctgg
    48421 cgatgaccgc gatcctcggt tatctggtca atagtgcggt gagaacgctg ctatttggct
    48481 actttggacg caaaatttat cgccccggtc tgcacttctc gctggcgtca gttgcgccga
    48541 acttacgctt tggtgcctgg ctgacggcgg acagcatcat caactatctc aacaccaacc
    48601 tttcgacgct ggtgctggcg cgtattctcg gcgcgggcgt ggcaggggga tacaacctgg
    48661 cgtacaacgt ggccgttgtg ccgccgatga agctgaaccc aatcatcacc cgcgtgttgt
    48721 ttccggcgtt tgccaaaatt caggacgaca ccgaaaagct gcgcgtcaac ttctacaagc
    48781 tgctgtcggt ggtggggatt atcaactttc cggcgctgct ggggctgatg gtggtgtcga
    48841 ataactttgt accgctggtc tttggtgaga agtggaacag cattattccg gtgctgcaat
    48901 tgctgtgtgt ggtgggtctg ctgcgctccg taggtaaccc gattggctcg ctgctgatgg
    48961 cgaaagcgcg ggtcgatatc agctttaaat tcaacgtatt caaaacattt ctgtttattc
    49021 cggcgattgt tataggtggg cagatggcgg gcgcaatcgg cgtcacgctt ggtttcctgc
    49081 tggtgcagat tatcaacacc attctgagtt acttcgtgat gattaaaccg gttcttggtt
    49141 ccagttatcg ccagtacatc ctgagtttgt ggctgccgtt ttacctctcg ctgccgacgc
    49201 tgggggtcag ttatgcgctg ggcattgtgc tgaaagggca actggcgctg gggatgcttt
    49261 tggcggtgca aatagccgcg ggcgtgctgg cgtttgtggt gatgattgtg ctgtcgcgcc
    49321 atccgctggt ggtggaagtg aagcgtcagt tttgtcgcag cgaaaaaatg aaaatgcttt
    49381 tacgggcggg gtgagttgtt gttttcggtt ccgacaacgg ttgccgggtg cagcgtaaaa
    49441 cgccttatcc atcctacggt tctgtgctcg ggacctgtag gcctgataag acgcgtcagc
    49501 gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcag
    49561 ggcttggatc ttgtagataa gacgcgccag cgtcgcatca ggcactgaat gccggatgcg
    49621 gcgtaaaacg ccttatccgg cctacggttc tgtgctcggg tcttgtaggc ctgataagac
    49681 gcggccagcg tcgcatcagg caaaaaacgg caatgaacac aacaaacaca gcctcacctg
    49741 gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca cttgcggcaa
    49801 tcgtggcgac agcgccatcc tgcgcggctt acttgatgcc atcaacatcc tcaatccaca
    49861 agccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc tcaaccgccc
    49921 ggtaatgggc gatccgctgt tcctgcaaat gaaacaacac aatagcgcgg cgggcgttgt
    49981 cgggcgcgtt aaaaaagtcc tccgtcgccg ctatcagcac caggtattgc tctcacgcgt
    50041 caccgacact ggcaagctgc gtaatatcgc catcgcccag ggattcaccg acttcgtgcg
    50101 cctgctgtca ggttacgacg ccattattca ggtcggcgga tcgttttttg tcgatctcta
    50161 cggcgtgcca cagtttgaac atgcgctttg cacatttatg gcgaaaaagc cgctgtttat
    50221 gattggtcac agcgttggcc cgttccagga tgagcaattt aaccaactgg cgaactatgt
    50281 ctttggtcac tgtgacgcgc tgatcctgcg cgaatcggtc agccttgatt tgatgaaacg
    50341 cagcaatatc actaccgcaa aagttgaaca tggcgttgat actgcctggc tggtcgatca
    50401 tcacacagaa gaattcaccg ccagctatgc cgttcaacac tggctggacg ttgccgcaca
    50461 acagaaaacg gtggcaatta ccctgcgcga actggcaccg ttcgacaaac gtctcggcac
    50521 cactcagcaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc tcgatgaagg
    50581 gtatcaggtg attgcgcttt ccacctgtac gggcatcgac agttataaca aagacgatcg
    50641 catggtggcg ctcaatctgc gccagcacat cagcgatcct gcccgttacc acgtagtgat
    50701 ggatgaactc aacgatctgg aaatgggcaa aattctgggt gcctgtaaac tcaccgtcgg
    50761 tacgcgcctg cactctgcca ttatctcgat gaactttgcc actccggcga ttgccatcaa
    50821 ctatgaacat aaatccgccg ggattatgca acaactgggg ctaccagaga tggcaattga
    50881 tattcgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct taggccagct
    50941 tccggcgctg aatgcgcgac ttagtgaagc cgtcagtcgt gagcgtcaga caggaatgca
    51001 gatggtgcag tccgtactgg aacgcatcgg ggaggtgaaa tgaaggtcgg cttcttctta
    51061 ttgaaatttc cgctgtcgtc ggaaaccttc gtcctcaacc aaatcactgc gtttattgat
    51121 atgggctttg aggtggagat tgtcgcgctg caaaaaggcg acacacaaaa cacccacgcg
    51181 gcatggacga aatacaacct tgccgccaga acccgctggt tacaggacga acctacgggc
    51241 aaagtggcga aactgcgcca ccgagccagt cagaccttgc gcggcattca tcgtaaaaat
    51301 acctggcagg cgctcaacct caaacgctat ggtgccgagt cgcggaacct gattttgtct
    51361 gccatttgcg gccaggtcgc aacaccattt tatgccgatg tctttatcgc tcattttggc
    51421 cctgcggggg taaccgcagc aaaactacgc gaactgggtg tcattcgcgg caaaattgcc
    51481 actatcttcc acggtattga tatctccagt cgggaagtgc tcaaccacta cactcccgaa
    51541 tatcaacaac tgtttcgccg tggcgacctg atgttaccga taagcgattt gtgggccgga
    51601 aggctgcaaa aaatgggctg cccgagggaa aaaattgccg tatcgcgtat gggcgtggac
    51661 atgacgcgtt ttagcccgcg tccggtgaaa ccgcccgcaa cgccgctgga aatcatttcc
    51721 gtcgcacgct taaccgagaa aaaaggcctg catgtggcga tcgaagcctg ccgtcagttg
    51781 aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg ggaacgacgc
    51841 ctgcgcacgc tcatcgaaca atatcaactg gaagatgtgg tggagatgcc gggctttaaa
    51901 ccgagccatg aagtgaaagc gatgctcgac gatgcggatg tcttcctctt gccatcggtt
    51961 acaggtgcgg acggcgatat ggaaggcatt ccggtggcgc tgatggaagc gatggcggtc
    52021 ggcattccgg ttgtttctac tctacatagc ggaataccgg aactggtgga ggccgataaa
    52081 tccggctggc tggtgcctga gaacgatgct cgcgcactgg cgcaacgcct ggcggcattt
    52141 agccaactgg acaccgacga actggctccg gtcgtcaaac gtgcacgcga aaaagtcgaa
    52201 cacgatttta accagcaggt gattaatcga gaactcgcca gcttgctgca ggctttatag
    52261 aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg caagctccgc
    52321 gcttgccttc ctccatacac ctttcgcccg tgcacttcct gcccgacaaa gcgttaacat
    52381 taacgactac aaccctcacg actggatcgc ctcatttaaa caagccttcg gcgaagggca
    52441 aacggtcgtc gtgcctgccg gattggtttg cgacaatatc aacaccggca tcttcattcc
    52501 tgcaggtaaa acgttacaca tccttggaag cctgcgcggc aacggcagag ggcgatttgt
    52561 cttacaggac ggcagccagg tgacaggggg ggagggcggc agtatgcata acatcaccct
    52621 ggatgtgcgc ggctctgact gcaccatcaa agggctgact atgagcggct ttggtccggt
    52681 aacgcagatt tatatcggcg gcaaaaacaa acgggtcatg cgcaacctga ccatcgataa
    52741 cctgaccgtt agccacgcta attacgccat cttacgccag ggatttcata accagattat
    52801 cggtgccaac atcaccaact gtaagttcag cgacttacaa ggcgacgcca ttgaatggaa
    52861 cgtggcaatt aacgacagtg atattttgat ctccgaccat gtcatcgagc gcatcaactg
    52921 tactaatggc aaaatcaact ggggcatcgg cataggcctt gcggggagta cctacgataa
    52981 taactacccg gaagaccagg cagtgaaaaa ctttgtcgtg gcgaatatca cgggatcgga
    53041 ttgtcggcag ttgatccatg ttgaaaatgg caaacatttt gttattcgta atatcaaagc
    53101 tcgcaatatc acgccggatt tcagtaagaa agcaggcatt gataacgcga cagttgctat
    53161 ttacggttgt gacaatttcg tgattgataa tattgaaatg attaatagcg ccgggatgtt
    53221 aatcggctat ggggtaatta aaggcaaata tctctcgata ccgcaaaatt tccgagtgaa
    53281 taatattcaa ctggataata cccaccttgc ttataaattg cgcggtatcc aaatctccgc
    53341 cgggaatgcc gtctcctttg tgtcgctaac taatattgag atgaagcgtg cctcgctgga
    53401 gttacacaac aagccgcaac atctttttat gcgaaatatc aaagtgatgc aggaatcctc
    53461 tgttggacca gcattgagca tgaacttcga catgcgcaaa gacgttcgcg gcgtctttat
    53521 ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg agaaaggaca
    53581 aagctccgtc gatatcgaca gggttaatca ccatattgtt aatgtggaaa agattaactt
    53641 tagattgccg gaacggaggg agtagatttg cgaccattca taaacttact ggatataatg
    53701 ttctaaaaga ttataatcct cacactaact gttaaagctg gggctgggca gcattgacta
    53761 ctcagggaag gtactcatct ttatcttgtg tttcttacgt aacaaccaga ctaaagtgat
    53821 ttatgaacga taacgttttg ctcataggag cttccggatt cgtaggaacc cgactacttg
    53881 aaacggcaat tgctgacttt aatatcaaga acctggacaa acagcagagc cacttttatc
    53941 cagaaatcac acagattggc gatgttcgtg atcaacaggc actcgaccag gcgttagccg
    54001 gttttgacac tgttgtacta cttgcagcgg aacaccgcga tgacgtcagc cctacttctc
    54061 tctattatga tgtcaacgtt cagggtaccc gcaatgtgtt ggcggccatg gaaaaaaatg
    54121 gcgttaaaaa tatcatcttt accagttccg ttgctgttta tggtttgaac aaacacaacc
    54181 ctgacgaaaa ccatccacac gatcctttca accactacgg caaaagtaag tggcaggcag
    54241 aggaagtgct gcgtgaatgg tataacaaag caccaacaga acgttcatta accatcatcc
    54301 gtcctaccgt tatcttcggt gaacgcaacc gcggtaacgt ctataacttg ctgaaacaga
    54361 tcgctggcgg caagtttatg atggtgggcg cagggactaa ctataagtcc atggcttatg
    54421 ttggaaacat tgttgagttt atcaagtaca aactgaagaa tgttgccgca ggttatgagg
    54481 tttataacta cgttgataag ccagatctga acatgaacca gttggttgct gaagttgaac
    54541 aaagcctgaa caaaaagata ccttctatgc acttgcctta cccactagga atgctgggtg
    54601 gatattgctt tgatatcctg agcaaaatta cgggcaaaaa atacgctgtc agctctgtgc
    54661 gcgtgaaaaa attctgcgca acaacacagt ttgacgcaac gaaagtgcat tcttcaggtt
    54721 ttgtggcacc gtatacgctg tcgcaaggtc tggatcgaac tctgcagtat gaattcgtcc
    54781 atgccaaaaa agacgacata acgtttgttt ctgagtaatg cagacgagtc agagaaaaaa
    54841 ggaccaggtt ggactacgat tattcggaaa gaggtactga ttaaattatc agtatgtgga
    54901 tttccgagta atccgagtaa cccagatgct gatggtgtgt agtgttatcg taaggtttaa
    54961 tcaaagttat gcggaactaa gctttggtat atagccataa cgtttggcta acctatggca
    55021 ataatttacg cactatcagc cacttagagg taggaaaaga atgacgaatt taaaagcagt
    55081 tataccggta gcaggtcttg ggatgcatat gttgcctgcc actaaggcga ttcccaaaga
    55141 gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga ttgtggctgc
    55201 agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg aaaaccactt
    55261 cgacacctct tatgaattag aatctcttct tgagcagcgc gtgaagcgtc aactgcttgc
    55321 ggaagtgcag tccatctgtc caccgggcgt gaccattatg aacgtgcgtc agggcgaacc
    55381 tttaggttta ggccactcca ttttgtgtgc acgacccgcc attggtgaca acccatttgt
    55441 cgtggtgctg ccagacgttg tgatcgacga cgcgagtgcc gatccgctgc gttacaacct
    55501 tgctgccatg attgcgcgct tcaacgaaac gggccgcagt caggtgctgg caaaacgtat
    55561 gccgggtgac ctctctgaat actccgttat tcagaccaaa gaaccactgg atcgtgaagg
    55621 taaagtcagt cgcatcgtcg aatttatcga aaaaccggat cagccgcaga cgctggactc
    55681 agatatcatg gccgttggtc gctatgtgct ttctgccgat atttggccgg aacttgaacg
    55741 cactcagcct ggtgcatggg ggcgtattca gctgactgat gccattgccg aactggcgaa
    55801 aaaacagtcc gttgatgcca tgctgatgac cggcgacagc tacgactgcg gtaaaaaaat
    55861 gggctatatg caggcgtttg tgaagtatgg actacgcaac ctgaaagaag gggtgaagtt
    55921 ccggaaaggt attgagaagc tgttaagcga ataatgaaaa tctgaccgga tgtaacggtt
    55981 gataagaaaa ttataacggc agtgaagatt cgtggtgaaa gtaatttgtt gcgaatattc
    56041 ctgccgttgt tttatataaa caatcagaat aacaacgagt tagcaatagg attttagtca
    56101 aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta acgtttgaat
    56161 ttttcgggtt tagcgcgggt gggtaacgcc cgtcacatcg taggcatgca tgcagtgctc
    56221 tggtagctgt aaagccaggg gcggtagcgt gcattaatac ctctattaat caaactgaga
    56281 gccgcttatt tcacagcatg ctctgaagta atatggaata aattaagtga aaatacttgt
    56341 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
    56401 ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
    56461 tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg ctgctgcaat
    56521 ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggctg ctgaaagcca
    56581 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtactta
    56641 tgtcctttta gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaaatag
    56701 tttccgtttt catcatattt ctactgacga agtctatggt gatttacctt atcctgacga
    56761 agtaaataat aaagaagaat tacccttatt tactgagaca acagcttacg caccaagcag
    56821 cccttattcc gcatcaaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
    56881 tggtttaccg accattgtga ctaactgctc taacaattat ggtccttatc atttcccgga
    56941 aaaattgatt ccattggtta ttctgaatgc tctggaaggt aaagcattac ctatttatgg
    57001 taaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
    57061 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
    57121 aaatatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc cgaaagagaa
    57181 atcttaccgc gagcaaatta cttatgttac cgatcgtccg ggacacgatc gccgttatgc
    57241 gattgatgct gagaagattg gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag
    57301 cgggattcgt aaaacagtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
    57361 aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
    57421 ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct ggcacctctg
    57481 ggtaatttga ttgctcttga tgttcactcc actgattact gtggtgattt tagtaatcct
    57541 gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa cgcagcagct
    57601 cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaattact taacgcgacg
    57661 agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg catgggttat tcactactct
    57721 actgactacg tatttccggg gaccggtgaa ataccatggc aggaggcgga tgcaaccgca
    57781 ccgctaaatg tttacggtga aaccaagtta gctggagaaa aagcattaca ggaacattgc
    57841 gcgaagcatc ttattttccg taccagctgg gtatacgcag gtaaaggaaa taacttcgcc
    57901 aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgattaa tgatcaattt
    57961 ggtgcgccaa caggtgctga gctgctggca gattgtacgg cacatgcaat tcgtatggca
    58021 ctgaataaac cagaagtcgc aggcttgtac catctggtag ccagtggtac cacaacctgg
    58081 cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggcattgc ccttgcactc
    58141 aacaagctca acgcagtacc aacaacagcc tatcctacac cagctcgtcg tccgcataac
    58201 tctcgccttg atacagaaaa atttcagcag aactttgcgc ttgtcttgcc tgactggaag
    58261 attggcgtga aacgcatgct caacgaatta tttacgacta cagctattta atagtttttg
    58321 catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
    58381 aaaggtatta ttttagcggg cggttctggt actcgtcttt atcctgtaac tatggctgtc
    58441 agtaaacagc tattaccgat ttatgataaa ccgatgatct attatccgct ctctacactg
    58501 atgttagcgg gtattcgcga tattctgatt atcagtacgc cacaggatac tcctcgtttt
    58561 caacaactgc tgggtgacgg gaaccagtgg ggcctgaatc ttcagtacaa agtgcaaccg
    58621 agtccggatg gtcttgcgca ggcatttatc atcggtgaag agtttatcgg tggtgatgat
    58681 tgtgctttgg tacttggtga taatatcttc tacggtcacg acctgcctaa gttaatggat
    58741 gccgctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa
    58801 cgctacggtg tcgttgagtt tgataaaaac ggtacggcga tcagcttgga agaaaaaccg
    58861 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtg
    58921 gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tggaaattac cgatattaac
    58981 cgtatttata tggaacaggg gcgtttatcc gttgccatga tgggacgtgg ttatgcatgg
    59041 ctggacacgg ggacacatca aagtcttatt gaggcaagca acttcattgc aacaattgaa
    59101 gagcgtcagg ggctaaaagt ttcctgccca gaagaaattg cttaccgtaa agggtttatt
    59161 gatgctgagc aggtgaaagt attagctgaa ccgctgaaaa aaaatgctta tggtcagtat
    59221 ctgctgaaaa tgattaaagg ttattaataa aatgaacgta attaaaacag taattccaga
    59281 agtcttgatt tttgagccta aaaaatttga ggactccagg gggtattttt ttgaaagctt
    59341 taatcaaagg ctttttgaag atgccgtagg aaaagaaact ctattcgtcc aagataatca
    59401 atcatattca agtaaaaatg tattaagagg tttacattat caaagcgagc cgtatgctca
    59461 agggaagcta gtaagatgta ttatggggga agtatttgat gtagccgtag atattagaaa
    59521 agagtcagaa agttatgggc aatgggttgg agtattttta tctgaggata ataatagaca
    59581 gttatggata cctgaaggat ttgctcacgg ttttcttgtt aaaagcgata aagcaatttt
    59641 t
//
LOCUS       DAZFFI010000014        59020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFFI010000014 DAZFFI010000000
VERSION     DAZFFI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.014"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHJ12_001132"
     CDS             complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHJ12_001132"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4988956.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1371..1769
                     /gene="yohJ"
                     /locus_tag="ACHJ12_001133"
     CDS             1371..1769
                     /gene="yohJ"
                     /locus_tag="ACHJ12_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4988957.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1766..2461
                     /gene="yohK"
                     /locus_tag="ACHJ12_001134"
     CDS             1766..2461
                     /gene="yohK"
                     /locus_tag="ACHJ12_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4988958.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2591..3475
                     /gene="cdd"
                     /locus_tag="ACHJ12_001135"
     CDS             2591..3475
                     /gene="cdd"
                     /locus_tag="ACHJ12_001135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4988959.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3625..4344
                     /gene="sanA"
                     /locus_tag="ACHJ12_001136"
     CDS             3625..4344
                     /gene="sanA"
                     /locus_tag="ACHJ12_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4988960.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4347..4586
                     /gene="yeiS"
                     /locus_tag="ACHJ12_001137"
     CDS             4347..4586
                     /gene="yeiS"
                     /locus_tag="ACHJ12_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4988961.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4905..6143
                     /gene="preT"
                     /locus_tag="ACHJ12_001138"
     CDS             4905..6143
                     /gene="preT"
                     /locus_tag="ACHJ12_001138"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4988962.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            6137..7372
                     /gene="preA"
                     /locus_tag="ACHJ12_001139"
     CDS             6137..7372
                     /gene="preA"
                     /locus_tag="ACHJ12_001139"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4988963.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7443..8453)
                     /gene="mglC"
                     /locus_tag="ACHJ12_001140"
     CDS             complement(7443..8453)
                     /gene="mglC"
                     /locus_tag="ACHJ12_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4988964.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8469..9989)
                     /gene="mglA"
                     /locus_tag="ACHJ12_001141"
     CDS             complement(8469..9989)
                     /gene="mglA"
                     /locus_tag="ACHJ12_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4988965.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10050..11048)
                     /gene="mglB"
                     /locus_tag="ACHJ12_001142"
     CDS             complement(10050..11048)
                     /gene="mglB"
                     /locus_tag="ACHJ12_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4988966.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11328..12368)
                     /gene="galS"
                     /locus_tag="ACHJ12_001143"
     CDS             complement(11328..12368)
                     /gene="galS"
                     /locus_tag="ACHJ12_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4988967.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(12510..13667)
                     /gene="yeiB"
                     /locus_tag="ACHJ12_001144"
     CDS             complement(12510..13667)
                     /gene="yeiB"
                     /locus_tag="ACHJ12_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4988968.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13684..14352)
                     /gene="folE"
                     /locus_tag="ACHJ12_001145"
     CDS             complement(13684..14352)
                     /gene="folE"
                     /locus_tag="ACHJ12_001145"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4988969.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14610..15446
                     /gene="yeiG"
                     /locus_tag="ACHJ12_001146"
     CDS             14610..15446
                     /gene="yeiG"
                     /locus_tag="ACHJ12_001146"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4988970.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15478..17470)
                     /gene="cirA"
                     /locus_tag="ACHJ12_001147"
                     /pseudo
     CDS             complement(15478..17470)
                     /gene="cirA"
                     /locus_tag="ACHJ12_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(17763..19232)
                     /gene="lysP"
                     /locus_tag="ACHJ12_001148"
     CDS             complement(17763..19232)
                     /gene="lysP"
                     /locus_tag="ACHJ12_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4988971.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19437..20318)
                     /gene="yieE"
                     /locus_tag="ACHJ12_001149"
     CDS             complement(19437..20318)
                     /gene="yieE"
                     /locus_tag="ACHJ12_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4988972.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20417..21466
                     /gene="yeiH"
                     /locus_tag="ACHJ12_001150"
     CDS             20417..21466
                     /gene="yeiH"
                     /locus_tag="ACHJ12_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4988973.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21540..22397
                     /gene="nfo"
                     /locus_tag="ACHJ12_001151"
     CDS             21540..22397
                     /gene="nfo"
                     /locus_tag="ACHJ12_001151"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4988974.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22401..23489
                     /locus_tag="ACHJ12_001152"
     CDS             22401..23489
                     /locus_tag="ACHJ12_001152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4988975.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23545..24795)
                     /gene="nupX"
                     /locus_tag="ACHJ12_001153"
     CDS             complement(23545..24795)
                     /gene="nupX"
                     /locus_tag="ACHJ12_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4988976.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24895..25836)
                     /gene="rihB"
                     /locus_tag="ACHJ12_001154"
     CDS             complement(24895..25836)
                     /gene="rihB"
                     /locus_tag="ACHJ12_001154"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4988977.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDIDWFWGLVEECVRGYIKTH"
     gene            25966..26664
                     /gene="yeiL"
                     /locus_tag="ACHJ12_001155"
     CDS             25966..26664
                     /gene="yeiL"
                     /locus_tag="ACHJ12_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4988978.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARNYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26735..27985)
                     /locus_tag="ACHJ12_001156"
     CDS             complement(26735..27985)
                     /locus_tag="ACHJ12_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4988979.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGSKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28079..29017)
                     /gene="psuG"
                     /locus_tag="ACHJ12_001157"
     CDS             complement(28079..29017)
                     /gene="psuG"
                     /locus_tag="ACHJ12_001157"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4988980.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(29005..29946)
                     /gene="psuK"
                     /locus_tag="ACHJ12_001158"
     CDS             complement(29005..29946)
                     /gene="psuK"
                     /locus_tag="ACHJ12_001158"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4988981.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(30370..32061)
                     /gene="fruA"
                     /locus_tag="ACHJ12_001159"
     CDS             complement(30370..32061)
                     /gene="fruA"
                     /locus_tag="ACHJ12_001159"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4988982.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(32078..33016)
                     /gene="fruK"
                     /locus_tag="ACHJ12_001160"
     CDS             complement(32078..33016)
                     /gene="fruK"
                     /locus_tag="ACHJ12_001160"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4988983.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33016..34146)
                     /gene="fruB"
                     /locus_tag="ACHJ12_001161"
     CDS             complement(33016..34146)
                     /gene="fruB"
                     /locus_tag="ACHJ12_001161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4988984.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34514..35695
                     /gene="setB"
                     /locus_tag="ACHJ12_001162"
     CDS             34514..35695
                     /gene="setB"
                     /locus_tag="ACHJ12_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4988985.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35692..35946)
                     /gene="yeiW"
                     /locus_tag="ACHJ12_001163"
     CDS             complement(35692..35946)
                     /gene="yeiW"
                     /locus_tag="ACHJ12_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4988986.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36101..36673
                     /gene="yeiP"
                     /locus_tag="ACHJ12_001164"
     CDS             36101..36673
                     /gene="yeiP"
                     /locus_tag="ACHJ12_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4988987.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36896..38362
                     /gene="yeiQ"
                     /locus_tag="ACHJ12_001165"
     CDS             36896..38362
                     /gene="yeiQ"
                     /locus_tag="ACHJ12_001165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4988988.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38480..39466
                     /gene="yeiR"
                     /locus_tag="ACHJ12_001166"
     CDS             38480..39466
                     /gene="yeiR"
                     /locus_tag="ACHJ12_001166"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4988989.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39505..40218
                     /gene="lpxT"
                     /locus_tag="ACHJ12_001167"
     CDS             39505..40218
                     /gene="lpxT"
                     /locus_tag="ACHJ12_001167"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4988990.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40630..41196
                     /gene="mepS"
                     /locus_tag="ACHJ12_001168"
     CDS             40630..41196
                     /gene="mepS"
                     /locus_tag="ACHJ12_001168"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4988991.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41377..42933
                     /gene="pdeN"
                     /locus_tag="ACHJ12_001169"
     CDS             41377..42933
                     /gene="pdeN"
                     /locus_tag="ACHJ12_001169"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4988992.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43015..44829
                     /gene="yejA"
                     /locus_tag="ACHJ12_001170"
     CDS             43015..44829
                     /gene="yejA"
                     /locus_tag="ACHJ12_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4988993.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            44830..45924
                     /gene="yejB"
                     /locus_tag="ACHJ12_001171"
     CDS             44830..45924
                     /gene="yejB"
                     /locus_tag="ACHJ12_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4988994.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGGRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45924..46949
                     /gene="yejE"
                     /locus_tag="ACHJ12_001172"
     CDS             45924..46949
                     /gene="yejE"
                     /locus_tag="ACHJ12_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4988995.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46951..48540
                     /gene="yejF"
                     /locus_tag="ACHJ12_001173"
     CDS             46951..48540
                     /gene="yejF"
                     /locus_tag="ACHJ12_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4988996.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGAPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48544..48888)
                     /gene="yejG"
                     /locus_tag="ACHJ12_001174"
     CDS             complement(48544..48888)
                     /gene="yejG"
                     /locus_tag="ACHJ12_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4988997.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49222..50412)
                     /gene="bcr"
                     /locus_tag="ACHJ12_001175"
     CDS             complement(49222..50412)
                     /gene="bcr"
                     /locus_tag="ACHJ12_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4988998.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(50440..51135)
                     /gene="rsuA"
                     /locus_tag="ACHJ12_001176"
     CDS             complement(50440..51135)
                     /gene="rsuA"
                     /locus_tag="ACHJ12_001176"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4988999.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51284..53044
                     /gene="radD"
                     /locus_tag="ACHJ12_001177"
     CDS             51284..53044
                     /gene="radD"
                     /locus_tag="ACHJ12_001177"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4989000.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53169..53453
                     /gene="rplY"
                     /locus_tag="ACHJ12_001178"
     CDS             53169..53453
                     /gene="rplY"
                     /locus_tag="ACHJ12_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4989001.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53592..54599)
                     /gene="yejK"
                     /locus_tag="ACHJ12_001179"
     CDS             complement(53592..54599)
                     /gene="yejK"
                     /locus_tag="ACHJ12_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4989002.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54781..55008
                     /gene="yejL"
                     /locus_tag="ACHJ12_001180"
     CDS             54781..55008
                     /gene="yejL"
                     /locus_tag="ACHJ12_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4989003.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            55028..56788
                     /gene="yejM"
                     /locus_tag="ACHJ12_001181"
     CDS             55028..56788
                     /gene="yejM"
                     /locus_tag="ACHJ12_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4989004.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56863..56939
                     /locus_tag="ACHJ12_001182"
     tRNA            56863..56939
                     /locus_tag="ACHJ12_001182"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56897..56899,aa:Pro,seq:ggg)
     gene            57158..58399
                     /locus_tag="ACHJ12_001183"
     CDS             57158..58399
                     /locus_tag="ACHJ12_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4989005.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRVVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            58498..58728
                     /locus_tag="ACHJ12_001184"
     CDS             58498..58728
                     /locus_tag="ACHJ12_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989006.1"
                     /translation="MQKNFRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNMAESIDRVVRPATMKTGGRPKKWR"
BASE COUNT        14620 a        15362 c        14532 g        14506 t
ORIGIN      
        1 cttatccctt acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa cgcgccctca
       61 gcccacatcc atgtgggctg ccccggcctt cagggaacgc ctcggcaatt ttgacgccac
      121 caaacaaccg tgcggcctat tgataaagag ctaacacatt gtcaaaaaac atcactatgg
      181 ttttttagag tttctcgata tcaattgcct gaatagccct tgcaatatca ggggaattat
      241 tcaacacccg aacatgctga aataatcccg ttgcttcatc gtattcttta cgcaaataac
      301 tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc ttttccagac
      361 gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt tcgttatatt
      421 ttactacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
      481 agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc ccagatttcg ccgttggcaa
      541 tcaccggaat attcagccgc tggcgaatct cgccaatcgc ctgccagtca atatgctccg
      601 cgcggtaacc ctgctctttt gtccgtccat gcaccaccag ctccgtagca ccagcctgtt
      661 gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttca
      721 cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc ttttgcaccc tgatagatga
      781 gttcaggatc tttgagtaac gtcgccccac caccgctacc gttgaccgtt ttcgacgggc
      841 agccgcaatt gagatccacg ccccaggaac ctaattccac cgcacgggcg gcgttctctg
      901 ccagccattg tgggaactga cccaacaact gcacgcgcac cagcgtaccg gatggtgtcc
      961 ggctggcgtt ttgtagctca gggcaaatgc gatgaaaaac ttttaccggc agcagttgat
     1021 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
     1081 cacgcaccag agagtcaagc actccctcca tcggtgccag taacacacgc atatcatcac
     1141 ccgcaaaata atgaggcgct atgttagcgc ctatggtcag cggattaaag gtctacaaag
     1201 agggattgct gcaaactgca atccctctct atgcttgatg tatgcctgag ttcgttgcgc
     1261 attgcgtaat gccggggagc gaaaaaataa gcgctgattt cttaatgtga tcggtagcac
     1321 gttttacgaa ttaattgtat gatgaatcca tctcatctgg ggtgttgatt atgagtaaga
     1381 cactgaacat tatctggcaa tatttacgcg ctttcgtcct gatttatgcc tgcctgtatg
     1441 caggcatttt cattgcttcc ctgctaccgg taaccattcc gggcagcatc atcgggatgc
     1501 tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc gaaatgggtc aatccggggt
     1561 gctacgtact gattcgctat atggcgctat tgtttgtgcc gattggcgta ggcgtcatgc
     1621 aatattttga tttgctccgc gcgcagtttg gcccggtagt ggtttcctgt gctgtcagta
     1681 cgctggtggt ttttctggtg gtgagctgga gttcacaact ggtacacggt gaacgtaaag
     1741 tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc tggtggtcat taccgctgac
     1801 gttgattgtc ttttttgccg cccgcaaact ggcggcacgg tataagtttc ccctgcttaa
     1861 cccgttgctg gtagcaatgg tggtgatcat tcctttttta atgctgaccg gcatctctta
     1921 cgacagctac tttaaaggta gcgaagtgct aaacgatctg ctgcaaccgg cggtcgtcgc
     1981 gctggcctat cctttatatg agcagctaca ccagatccgc gcgcgctgga aatcgatcat
     2041 caccatctgt tttatcggca gcgtggttgc gatggtgacg gggacttccg tggcattgtt
     2101 gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca ccacgcctat
     2161 tgcaatggcg gttggcggca gtattggcgg tattccggca atcagcgcag tttgcgtgat
     2221 tttcgtcggc atcctcggcg cggtatttgg ccatacattg cttaatgcga tgcgtattcg
     2281 taccaaagct gcgcgcggtc tggcaatggg gactgcctcg cacgccctcg gtacggcgcg
     2341 ctgcgccgag ctggattatc aggaaggtgc atttagttcg ctagcgctgg tgttatgcgg
     2401 gataattact tcgctgatcg caccgttcct tttcccgatt attctggcag taatgggcta
     2461 aaatttgcga tgcgtcgcgc atttttgatg taagtttcac gcgttgcata attaatgaga
     2521 ttcagatcac atataaagcc ataacgggtt cgtaaactgt tatcccatta catgattatg
     2581 aggcaacgcc atgcatccac gttttcaaac cgcttttgcc caacttgcgg ataacttgca
     2641 atctgcactg gaacctattc tggcagacaa gtacttcccc gctttgttga ccggggagca
     2701 agtctcatcg ctgaagagcg caacggggct ggacgaagac gcgctggcat tcgcactact
     2761 tccgctggcg gcggcctgtg cgcgtacgcc attgtcgaat tttaatgttg gcgcaattgc
     2821 gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg gaatttattg gtgcgacaat
     2881 gcagcaaacc gttcatgccg aacaaagcgc gatcagccac gcctggttga gtggtgaaaa
     2941 agcgcttgca gccatcaccg ttaactacac gccttgtggt cactgccgtc agtttatgaa
     3001 tgaactgaac agcggtctgg atctgcgtat tcatctgccg ggccgcgagg cacacgcgct
     3061 gcgtgactat ctgccggatg cctttgggcc gaaagatctg gagattaaaa cgctgctgat
     3121 ggacgagcag gatcacggct atgcgctgac tggtgatgcg ctttctcagg cggcgattgc
     3181 agcggcaaac cgttcgcaca tgccttacag taagtcgcca agcggtgtcg cgctggaatg
     3241 taaagacggt cgtattttca gtggcagcta cgctgaaaac gccgcattca acccgactct
     3301 gccaccgttg cagggagcgt taattctgtt gaatctcaag ggttatgatt acccggatat
     3361 ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg attcagtggg atgccacctc
     3421 cgcaacgctg aaagctctcg gctgtcacag tatcgaccga gtgcttctcg cttaagcctg
     3481 gtgccggatg cggcgtgaac gccttgtccg gcttgccagc cctctcctgg tgtcgaaatt
     3541 cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg gtaaagtagc ctgatggaaa
     3601 ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt tcctcagcct gttagtcctg
     3661 atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct ggatgagctg gaaaaccgcg
     3721 ccttatatct acgacgaatt gcaggatctc ccctaccgcc aggtcggtgt ggtgctcgga
     3781 acagcaaaat attatcgtac tggcgtaatt aatcagtatt atcgctaccg cattcaagga
     3841 gcgattaatg cctataacag cggtaaggta aattatctat tactgagcgg cgataacgca
     3901 ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt taatcgctgc tggtgtcgac
     3961 ccatcagata ttgttctcga ttacgcaggc tttcgtacgc tggattccat cgtgcgtaca
     4021 cgcaaagttt tcgatactaa tgatttcatt attatcaccc aacgtttcca ctgtgagcgg
     4081 gcattattta ttgcgctgca tatggggatt caggctcagt gttatgccgt accgtcaccg
     4141 aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc gtttcggtgc gctggctgac
     4201 ctttatattt ttaaacgtga accgcgtttt ttagggccgc tggtccctat tccggctatg
     4261 caccaggtgc cggaagatgc gcaggggtat cccgccgtca cacccgaaca gttacttgaa
     4321 ttacaaaaga aacaaggaaa gtagttatgg atgtacagca gttttttgtc gttgccgttt
     4381 ttttccttat tccgatattt tgtttccgcg aagcatggaa aggctggcgc gcaggcgcga
     4441 ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt ctggcgagca aaaaatcccg
     4501 gactcttttt cgcttatatg gtggcatata tcggcttcgg aattttatct atcggcatga
     4561 ttgtttatct tattttctat cgttaatatt ttctcttttc tgttaattat tgagaataat
     4621 tattacttca ctcaataagc tgcggatata acattctgaa ccgcagctat ttgtgaatcc
     4681 tttcacagta taaatttccc cacgcgcata gccttaatat cagcacgcaa tatttatact
     4741 ctaaataatt cgagttgcag gaaggcgaca agtgagtgag tccccaggag cttacataag
     4801 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta tgacgagtat
     4861 atttagcgtt cgccttaatt actcatagca ttaaggaaga tcacatgccg caacaaaatt
     4921 atctggatga actcactccg gcttttacgc ctttactggc gattaaagaa gcctctcgct
     4981 gtttattatg tcatgacgct ccctgtagtc aggcttgccc ggcacagacc gatccgggga
     5041 aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc tgccgagaca attcgcgaaa
     5101 ataatgccct cggtgccgtt tgtgccagag tgtgcccgac ggagaaatta tgtcaaagcg
     5161 gttgtacccg tgccggtgtt gatactccca ttgatatcgg tcgcttacag cgttttgtta
     5221 ctgattttga acaacaaacc gggatggaaa tttatcagcc cggtactaaa acgctcggca
     5281 aagtcgcgat tattggcgca ggtcctgccg gattgcaggc cagtgtaaca ctgacaaacc
     5341 agggttatga cgtcacgatt tatgagaaag aagcgcaccc cggtggctgg ttgcgtaacg
     5401 gtattccgca attccggtta ccgcaatcag tgctggatgc agagatcgcc cgtattgaaa
     5461 aaatgggcgt gaccattaag tgcaacaacg aagtcggtaa cacactcacc cttgagcagc
     5521 tgaaagcaga aaaccgcgcg gtactggtca ccgtggggtt atcaagcggt tccgggctac
     5581 cgctgtttga gcatagtgac gttgagattg ccgtcgactt cctgcaacgt gtacgacagg
     5641 cgcaaggcga tatcagcatt ccacaaagcg cattaattat cggcggcggt gatgtcgcga
     5701 tggacgtagc cagcacgctg aaagttctcg gctgtcaggc ggtaacctgc gtagcgcgtg
     5761 aagagttaga tgagtttccg gcaagcgaaa aagagtttgc cagcgcccgg gagctgggcg
     5821 tttcgatcat cgatggattc acgccagtag ccgtggaagg caataaagtc acgtttaagc
     5881 atgtacggtt accgggcgaa ctgacgatgg cggcagataa aattattctc gccgtcggtc
     5941 agcatgccag actggatgcc tttgcggagt tagagccgca gcgtaacacc atcaaaacac
     6001 aaaattatca gacccgcgat ccgcaagtct ttgctgctgg cgatattgtt gagggtgaca
     6061 aaaccgtggt ctatgccgtg aaaaccggga aagaagccgc cgaggcgatt catcactatt
     6121 tagagggagc ttgctcatgt taacgaaaga tctttcgatt actttttgcg gcgtgaagtt
     6181 tcccaacccg ttctgcctct cttcttcgcc tgtaggcaac tgctatgaga tgtgtgccaa
     6241 agcctacgac acaggttggg gcggcgtggt gtttaaaacg atcggctttt ttatcgccaa
     6301 cgaagtctcg ccgcgttttg atcatctggt gaaagaagat accggtttta tcggcttcaa
     6361 aaatatggag cagattgctg aacatccgtt ggaagagaat ctggccgccc tgcgtcagct
     6421 gaaggaagat tacccggaca aagtattgat cgcttcgatc atgggggaaa atgagcagca
     6481 atgggaggag ctggcgcgcc tggtgcaaga agctggcgcg gatatgatcg agtgtaactt
     6541 ctcctgtccg caaatgactt ctcatgcgat gggtagcgat gtcgggcaaa gcccggagct
     6601 ggtagaaaaa tattgtcggg cagtgaaacg gggttccacg ctgccaatgc tggcgaagat
     6661 gacgccgaat atcggtgata tgtgcgaagt ggcgctggcg gcgaagcgcg gcggcgcaga
     6721 tggcattgcg gcgattaaca ccgttaaatc catcaccaat atcgatctta atcagaaaat
     6781 cggtatgccg atcgttaacg gaaaatcgag tatttccgga tattccggta aagcggtaaa
     6841 accgatcgcc ctgcgcttca ttcagcagat gcgcacccat ccagaactgc gcgatttccc
     6901 aatcagcggt atcggcggca ttgaaacctg ggaggatgcg gctgagtttt tattgctcgg
     6961 cgcagcaacg ttacaggtga ccaccggcat catgcagtac gggtatcgga tagtggaaga
     7021 tatggcaagc gggttgtcgc attatctcgc cgatcaggga tttgattcgc tgcaggagat
     7081 ggtaggtctg gcgaataaca atattgtccc ggcggaagat ttagaccgca gttatattgt
     7141 ctatccccgt atcaatcttg ataaatgtgt tggctgtgga cgctgttata tttcctgtta
     7201 cgacggcggt caccaggcga tggaatggag cgagaaaacc cgcacaccgc attgtaatac
     7261 cgagaaatgt gtgggttgtc tgctttgtgg tcacgtctgc ccggtgggtt gtattgagct
     7321 cggggaagtg aagtttaaga aaggcgagaa agaacacccg gtaacgttgt aaaaccagag
     7381 acgcatccgg catttggcac cgattgccgg atgcagcgca tccggcaata tccacaatca
     7441 catcacttct tacgcgcgta tttcagtgaa tccagcgcta cggcgaagat aataatcgcc
     7501 cctttgatga tgtactgcca gtatgggttt acgccgatat acgtcaggcc atagttgatg
     7561 acggtaaaaa taattacccc ggtcaccacg ccaatcaccg tccccacacc gccgctgaac
     7621 gatacaccgc ctaccacgca cgccgcgata gcatccagct catacataaa gccgaggttg
     7681 ttggtggcag agccgatacg tccggcttct aacatcccgc caaaggcata gaacacgcca
     7741 gacaacgcgt agatcatcag caggttcagg ccgacgttga caccagatac ttttgccgct
     7801 tccgggttac cgccaatggc aaaaatgttc ttaccgaagc gggttttgtt ccacaacacc
     7861 cagacgaacg ccaccgcaat caacgcgtag aaggtgatgt aagagagacg gaaactcccc
     7921 agcgcgacaa agccctgagc aaaggtagag aagccactgt caaaaccaga aattggcgac
     7981 gccccgacaa agtcgtaata gagcgagttg atgccataga cgatgatcat cgtgccgagc
     8041 gtggtaatga acggcgtcac gttgagataa gcgataatca gaccgttgat caaaccgatc
     8101 accgcaccaa tggcacagac aatcagaata accagcgcaa tcggcatcgt cgccatttcc
     8161 gggaacactt tgttggcgtt atccatggac tgcaataatg tcgcagccac cactgccgcc
     8221 agccctacct gacgaccagc agaaagatcg gtcccctggg tgacaattaa ccctgccaca
     8281 ccgagcgcga taataatacg caccgatgac tgggtgagaa tattacttaa gttcaacaga
     8341 cttaaaaatg ttgggtcctg gaaaataata atcgccagca aaactaaaag aacgacgtaa
     8401 ataccgccct ctttcaggta agtaagaaaa cttttcttat ttaacgcact catgggaagc
     8461 ccctaatctt aaaggtgcaa agacgcaaga cgcagaattt cgttttgcgt tgttgttttt
     8521 gtatcgacaa ttccggaaac gagaccattg ctcatgacca gaatacggtc tgttatccct
     8581 aacaactcag gcatttcaga ggagataata ataatcccct tgcctttctt cgccagttcg
     8641 gcaattaact gataaatttc aaacttcgcc ccgacatcaa taccgcgcgt cggttcatcg
     8701 agcattaata tttctggttg cgttagtagc cagcgaccaa taatcacctt ttgctgatta
     8761 ccaccggaga gcgaaccaat ttgcgtccga tgacccggcg tttttacccg catcgaatca
     8821 atcacccact gggtatcgct tttcatccgc gagttatcca gtaaaccaac tttatttttg
     8881 tagttgcgaa tattggaaat taacgagtta aaaccaatat ccagataggc ataaattccc
     8941 gttgagcggc gctcctcagt taccagagca aatccgtggt ttatggcttc attggcatta
     9001 tggttattga tctttttgcc gtgcaacgta atggtgccag ccgatttctc gcgaatacca
     9061 aataacgtct caacaatatc ggtacgtttc gcccccacca gcccggcaat accgaggatc
     9121 tcccctttat gcagatcaaa cgagacatcg cgaatcgacg gctggcgcag tgacgtcagg
     9181 ttacgcacct cgaggatgac ttcgcccggc ttgttttctt tgtcagggaa acgctgatta
     9241 agagaacgcc caaccatcat ggcgatgatc ttgtccatcg tcagtcctgc cagcggttcg
     9301 gtggcgatcc actgaccgtc gcgcaatacg gtaacttcat cacataactg gaagatttct
     9361 tccattttat gcgagatata aacaataccg cagccgcgct cttttaattt acgaataata
     9421 gtgaacagat gattgacctc tttttcggtt aacgaagaag tcggttcatc cataatcaca
     9481 attttcgcgt tataggaaaa cgctttggcg atttcgatca tctgcatttg cgaaacggat
     9541 aatgtgccga cgcgcgcacg cggatcgata tcaatatcca gttcgtcaaa aatcgctttg
     9601 gtttcgcggt acattttgtc ctgatcgaca aacatgcctt tggtgggata tcgccccagc
     9661 cacatgttat ccatcaccga acgttgtaat accaggttta actcctggtg taccatcgaa
     9721 ataccatttt ccagtgcttc tttggcagaa tggaaatcga tctctttacc ctggaataaa
     9781 atggtgccgg agtctttttg ataaatacca aacaggcatt ttaataatgt tgattttcct
     9841 gcaccgtttt cccccattaa tgcatggata gaatgtggcc ggacttttaa attaacgtta
     9901 tcaagtgcct taacaccagg aaaggacttg ttgataccgc tcatttccaa caagtattcc
     9961 ccggaagacg gagtcgttga gctgaccata taattttacc ttgttggcca tacaataagg
    10021 gcgcagtaaa cgactgcgcc caatcagtct tatttcttgc tgaattcagc caggttgtct
    10081 ttatctacgc caacataagg tacgcggacc actttgttgt cgattttcca gttggtgcca
    10141 tcagccgcac ctttgccatc ggccaggttt ttcgccagat caaaggtcgc tttcgcctgg
    10201 ttgttagcat cgttcagtac tgtgcccgcc agtgcaccgg atttcaccag cgccagcgct
    10261 tctggcagcg catcgacgcc aaacaccgga atgctggact tgttgtgtgc tttcagtgct
    10321 tctaccgcgc ccattgccat cgcatcgttg ttggcgataa ccacttcgat tttgttggcg
    10381 ttcgggccag acagccaggc gtccatctta tctttcgcct gagcggtatc ccacatagcg
    10441 gtatctaact gtaactgttc agttttaatg cctttgtcgt tcagctcttt aatcacgtaa
    10501 gtggtacgtg cttctgcatc cggatggccc ggttcacctt tcagcaatac gaattgaatc
    10561 tgaccgtctt tgttcagatc ccaaccctga ttcgccgccc agtgtttagc aatcaaatcg
    10621 ccctgaataa tgccggactc tttggaatca gtgccaacgt agtaggcttt gtcgtagcta
    10681 tccagcgcct tacgagacgg ttctttgttg aagaaaacca ccggcacgtt ttgcccacgc
    10741 gctttctcaa tcaccgtacc cgcagctgcc gggtcaacca ggttgattgc cagtgccttc
    10801 acccctttcg ccagcaatac gtcgatctga tcgttctgct tggactggtc attctgagaa
    10861 tcattcatca gcagctgaac atctggcgcg gctttcgcat cttgctcaat agccttgcgc
    10921 actacagaca taaagttatc gtcgtactta tagattgtta caccaatgcg agtatcagca
    10981 gcgtgtgcag cggcaccgaa taacatgctg gccatcacag cagacagggt taacaccttc
    11041 ttattcatgg tatctccggt ttttcttatg cagagtagtg cttgagataa atgctcggcg
    11101 gggcagtaga gttaatgaag tgttactgaa cgccgaagct cactttttaa aattcgttct
    11161 tccatgcccg gtaacgctcc agaaaacggc tttaattgtt gttatgacgc tattacctcg
    11221 gtgaaagtga ttattcactg ttacatacgg gttacaacgt taaaacggtg caatcatagc
    11281 tatcacattg ttaagatact gtgaaatcac tcacagattg aaagcggtta catcgcctga
    11341 tttgttgagt tagtgatcgt cgccgcattc tggcgtgttg cgacagaatg gcgacgcact
    11401 aacgtcggca taaaacagtg gctggcacga ggatcaatat tgcctgctgc cccctgcaag
    11461 gccagttcgg tggctaattt cgccattgaa gcaatgggat aacgcacggt cgttaattgc
    11521 gggtcggtgt aacgggcgat gggaatatca tcgaaaccga tgattgagag atgtaacgga
    11581 atcgcaatgc cattatcttt taatgctgtc agtgcgccag cggccatatt gtcgttataa
    11641 gcaaatacag cggtaagttg tagattgcgc cccagcagtt caaccatcgc cgcctcaccg
    11701 cccggcatgt ccggcgtacc agtgccaatc cagctttccg gcggaataat atcctgctct
    11761 ttcaacgcac tcatccagcc tgctttacgc atggcgtcat cttcaatgcc gtggctggaa
    11821 gaaagataac caatacgttg atgaccgtta ttcagcaaca tgcgcgtcgc cattcgggca
    11881 ccgctgagat tatccaggca aacgcaacga tgggcgtacc ccggcacaac gcggttgatt
    11941 aacaccatac cgggaatgtt atccataaat tgcgccagtt catcgtcact caatgctttt
    12001 gagtgaacaa tcaacgcatt acaacgctgg cgaattaaca cctcaatggc gtgacgctct
    12061 ttttccgctt catgatagct attgccgatt agcacgtatt tctgatgctg ctgggcgacc
    12121 agatccaccg cttttaccag cgcgccgaaa aaagcatcag aaacgtccat caccaccacg
    12181 ccaatggtat cgctaacctg agttgccagc gcctgggcat tggcatttgg ccgataatcc
    12241 agctcactca cggctttcat tactgcttca cgcgtgtcgg cactgaccag cgtgctgtta
    12301 ttgagcaccc gggaaaccgt tgccacagag acgccagcct gacgcgctac atcacgaatg
    12361 gtgatcatat tcactacctg ttatgagatt gcagtaaatg actgcttgct ggcggctatt
    12421 ttgtcagcac tgaatacagg acttcgtgaa tcgagtcaca gcaatggaaa cggttacagc
    12481 cgttttgtta acgaatgtga tccagatcgt tatcttgatg ttttagatat tgccgttccg
    12541 gcagcacgca aagttaactg acgccagagc cattccaccg gcccctggcg gaagtaacgc
    12601 agccagataa cagagaagag gatattcgcc agccataccg gaataacaaa cgccagcagc
    12661 tccaggcgat caaaatgcat aaacaaaccg aggtggtaaa aaagcgtggt acaaatcagc
    12721 gtttgcaata gatagttggt cagcgccatc cgtccgacgc aggcgattgc cagcaccagc
    12781 ttaaagcggc ttaattgcgg ccagaagcca taaaacagcg acgcatagcc aatcgcctga
    12841 aacggcgcac tcagttcccg cggcatttga agtaagaagg cacaccagcg atacgcccag
    12901 tccagctgcc attgcagggc gatggcagga aggttaatgg tcaccccaat cgccaccagc
    12961 acaaaaccag tacgacgata gtgacgtaag ctgaactgcc ctttcagcca gccgctgcgc
    13021 atcagcgccg caccaatgag catcatccca gccagttgcc agccatactg tgcgcccagt
    13081 gccagtaaac tgttgccaac accatcggca cgattactga tcgcatcaac gccgccgtga
    13141 agcttccagt atttttcata caaaatagcc gatgcatccg gcgtccaggc gcggctggtc
    13201 tggctgtcgg aaatcagccc caataacagc aaaacgccaa gccccaccag ataaagcatg
    13261 acgccggtat taaacaggct ttttaccgat ggcgcatcgc gcaccagccg ccagcagatt
    13321 aagcccacca gcccgtaagc cagcagaata tcgccgtccc agaacaataa accgtgaata
    13381 aagcccaata agactaacag cgttaaccgc gactggatcc agcgtctgcc acggggcagc
    13441 aacatttgca gtccagcacc aaacagcagc gcaaaaagcg tgaggaattt cacctggccg
    13501 atgagatcaa gaaatgccca ggtccatgca tcctgcggcg taatagcacc gtaccaggcg
    13561 ggattgagat aagccgcctt tggtagccca aaggcgctga tgtttaatag caggatcccc
    13621 agaatggcga cgccgcgaac aaaatcgagc gtgacgttgc gctccatggt tcctgccttt
    13681 taatcagttg tgatgacgca cagcgcgcag aaactcgtgg cgcgtattct gactggattt
    13741 gaacaatcca ccaagagagg tcgttgttgt ggcactggtt gcatcgcgga tgccacgcgc
    13801 cttcacgcag taatgcaccg cgtcgatcga gacagccacg ttattggtgc ccagcagcgt
    13861 ttgtagcgca ataagaattt gctgcgtcag acgttcctgc acctgcggac gctgggcaaa
    13921 gaactgcacg atacggttaa tttttgacag accgatcacc gaatctttcg ggatataagc
    13981 caccgtcgct ttgccatcga tggtaacaaa atggtgttca caggtactgg tcagagtgat
    14041 atcgcgcacg gtgaccattt catcgacctt cattttgttt tcaatgaggg tgattttcgg
    14101 gaagttggcg taatccagac cggagaaaat ttcatcgaca tacattttag cgatgcgatg
    14161 cggcgtttcc atcaaactgt catcagccag gtcgagattc agcagctgca tgatttcggt
    14221 catatgacca gcaataaggc ttttgcgcgt ttcgttatcc atttcatgca cgggcgggcg
    14281 cagcggtgtt tccagtcctc gcgcaactaa cgcttcatga accagggccg cttctttact
    14341 gagtgatggc atttatgatt tctcctgcag gtgtgacgcc tccgccctgc gtgggggcaa
    14401 agttattaag ctgatttaca gcctgattat tgtgcgtgag gcggcgcaca taatccagta
    14461 ttcacagcga taattattgt aattgccgct gcctttcatc agcagatgtt aaaacatcgt
    14521 tatgcaaata cggaagtgaa agttactcac agcacattga ataaacggta tgatgaagaa
    14581 attgcaaaca acacaacaag gagccacgca tggaaatgct cgaagagcac cgctgttttg
    14641 aaggctggca gcaacgctgg cgacacgact ccagtacctt aaactgcccg atgacgttca
    14701 gtatctttct cccaccccct cgtgatcaca ccccgccacc agtgctgtac tggctttccg
    14761 gattaacctg caatgacgag aacttcacca ccaaggcggg tgcccagcgc gtggcggcag
    14821 aactggggat tgtactggtg atgccagaca ccagcccgcg cggcgaaaag gttgccaacg
    14881 acgatggcta cgatttaggc cagggcgcag gcttttatct taatgccacg caaccgccgt
    14941 gggcgacgca ttaccggatg tatgattatc tgcgcgatga attaccggcg ctggttcagt
    15001 cgcaatttaa tgtcagcgac cgctgcgcca ttagcggtca ctcaatgggt ggtcacggtg
    15061 cgctgattat ggcgctgaaa aatccgggta aatacaccag cgtttcggcc tttgcgccaa
    15121 ttgtgaatcc gtgcagcgtc ccgtggggaa tcaaagcgtt tagcagctat ttaggtgagg
    15181 acaaaaatgc atggctggaa tgggacagtt gcgcgctgat gtatgccagt aacgcgcagg
    15241 atgcgatccc gacgcttatc gatcagggcg ataatgatca gtttcttgcc gaccagttgc
    15301 aacctgcggt actggcagaa gccgcgcgcc agaaagcgtg gccgatgacg ctgcgtattc
    15361 agccgggata tgatcacagt tactacttca tcgcctcttt tatagaggat cacctgcgct
    15421 tccatgcgca gtatttactg aagtgaaagt ccgcccggtt cgccgggcat cttctcatca
    15481 gaagcgataa tccactgcca taaagtaacg acgtccgtct tcgttatagc tgtagtcgtc
    15541 acgactgaga tccttgtcgc caaggttcag cacgcctgcg cgcagtttga cgtctttagt
    15601 cacctgccag gccgcgccgg tattccagat ggtataaccg cccggtgttt tagccgtcgc
    15661 gctgtcggcg cgtttctgcc cggtatagtg accagaaaca tagaatgacc agtcttccag
    15721 cgccagcggt ttccagtcca gcgtaccgtt agcagtatgg aacggcagat cggatagcgg
    15781 tttgttttcg ccgttgctga catcacgacc atcgttgtag gtgtagttga tcgacagttt
    15841 ccattcatcg ttgaacggaa ttttcagttc ggtttccacg ccctgaatac gagctttgtt
    15901 aacgttgtag tagctaaata ccggtatgcg ccgtccgtta gcgcccgtct caaaaccaac
    15961 aaagttttgg tagcccggtg cagcgttgac gtcagacgta cggctgatgc tgatacgatc
    16021 tttcacatcg ttacggaaaa cggtaacgct ggattcaacc ccttccagcc agccttcttc
    16081 acccatgtag taaagcccca gctcccaact ttcgctggtt tctggtttca gatccgggct
    16141 acccacaatc ttacatgcgc cacggcagga attgctcgtc cagtcagggc taagttgcaa
    16201 cagagaaggt gctttaaatg ccgtcgccca gccccctttc accgttacgg tgtcggtggc
    16261 gttataaacc aggtaggcac gcggactcca gtgttcaccg taggtttcgt gatcgtccat
    16321 acgcacgccg gtcgtcagcg ccagcggctc gaagatccgc cattcatctt ccacaaacag
    16381 cgcgtactgg ctggcagacg ttttggagct ggttcccccg gtcaggttca ccgcatcgct
    16441 aagtttgtcg tgacgccatt caccgccaac cgtgagaaac tgattaatcg ccgtcagcgg
    16501 caacgtgtat ttgccgtcga ccgtattgct ttcggaagtt atcgggctgc tgttgccagg
    16561 gtttttgttc tcgactttct caccgtagta tttcagttcg ctggtgccgt aatcccaacg
    16621 cccattatgg ctgacggagt agttctggcg ttccaggcgg tttttgtcca gcgagtcgga
    16681 atcacgatcc tgacggtcga aaccgtatcc ggcagtaaaa tcgtgatttt gattcggtgt
    16741 ccaggcaaat tcgacattgc cgtcgcggct ggagaatcct tcaatacgcg gcgtttctcc
    16801 ggtatcggtg gtcgttgagt tttgcgggtc atccttttca cgttttgcca ggctgccgta
    16861 agctttcatt cccagcacac catcaattaa tggtccactg gtaaagaact gaccgttata
    16921 ggtatcaccg cgatcgcgat gttcctgaat ggtggtatcg acggtaacgg tacccgacca
    16981 tttctgaccg atttttttgg tgatgatatt cactacaccg ccgagcgcat cggagccgta
    17041 tagcgacgac atcgggccac gaaccacttc aatacgttcg atggaatcga ccgggatcca
    17101 gttcagatcc gaaatcattg tggcggaaga cggcattgcg ggagttaacg cgtttaccgt
    17161 cgacgagaat cagggtgtag ctgctgtcca gaccacgaat actaacgccc ttacggttat
    17221 ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc agattctgta
    17281 ccggttttcg ctgcaggtct tcctgggtaa tgacgctgat actggcgggt gcatctttga
    17341 ggttttgttc aacggaagat gcagtgacaa ccatcgtttc gccatcatca tcgaccgcta
    17401 acacaggcca tgcacaagaa atagcggaca aacacagccc gacccgtacg aaagggttca
    17461 acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggtt atcgctcaca
    17521 tcttcttcac gtccccttgc gtacggcagc atcgcggtgg acttatttgt atgcaggtga
    17581 tcatcccaga aagccggaac agcctctgat ttgtcagcct tttttgattg cggcgtaacg
    17641 ataatgcaaa cgataacaat tatcaatcca aatgttaaat tttatatccg catgatttgt
    17701 cagggaaatg gaattgaaaa agccctctcg gttgagaggg cttagcaagg aagggaggaa
    17761 acttatttct tatcgttctg cgggaacttc atttcgctgt aacgtacgaa gtgagttcct
    17821 ttaatcagct tgtagccgaa ccaaataatc aggaacagcg ggataccaat atacgttgcc
    17881 gctacgccgc cccagtcaat ggtatctttc aggaacgctt cgtagttctg gcccaaagtg
    17941 ataatcagac acagaatgaa tgcgaagatc ggccccagtg ggaagaaacc tgaacggtac
    18001 ggcagatcgt taatgtcgtg tccctgcaat acgtaaccgc gacggaagcg atagtggcta
    18061 atggcaatcc ccagccaggc gataaaaccc gtcatcccgg aggtgttcag cagccacagg
    18121 tataccgtct ggttgccaaa catggaggtc aggaagcaca gaccggcaat caccgtcgtc
    18181 gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat gcgcggcgct
    18241 ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc ggagttaccc
    18301 gctgacagca ccgccgtcag aataactgcg ttcatcaccg ccgccgcaga gagcagaccc
    18361 gcgtgctgga acaccagggt gaacggacta acgctgatgt ctttaacatc gttacgcagc
    18421 aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa cacatagaac
    18481 aacaggattc gccagaacac ctgacgtacc gcgcgtggaa tgtttttcgc cggatcttcg
    18541 gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc gacaatcata
    18601 gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat tgtccagttg
    18661 ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac gccaacgatg
    18721 ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc ttcaccaaag
    18781 ccacgaactg agatatagtt cagcaggaag ataacgccga ggaacaacgc actccagatc
    18841 cagcccggtg tatccgggaa ccaccagctc atgaccagct gagctgcaac caggtcaacg
    18901 gcgatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc aaagccttct
    18961 tcaacatagt tctgaccgta agtggcaaac gaaccggaaa ccggcatata tgcagccagt
    19021 tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcagcatata cgagagcaat
    19081 gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag acctgtaccg
    19141 atggaaccgc caatggcaat catcgtcagg tgacgcgcct ttaattcacg gcgtaagccc
    19201 ggcgcttctg tggttttagt ttcggaaacc atacaaaaat gctatccatc ttaaaaatga
    19261 ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt tataagacac
    19321 cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg cgaatgcatg
    19381 ctgagggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac agcaacttaa
    19441 cgcggtacat tcgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt ggaaaggtgt
    19501 ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg gaccgcaact
    19561 tcacttaatg tgcctgcctg caattgatct tcaatcaccc gtcgcgacag gcaactaatt
    19621 cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc catcgccatc
    19681 tcaaacttcg gtaaatgtga cagcaataga taatcgacaa tctcccgcgt gccggaaccg
    19741 cgttcacgca ggatccacgg cgcagcggcc agctgctcta aggtgaccgg accacgggcc
    19801 aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg ttcagaaata
    19861 atttcagtgc tgtggcacgg tccttcaata aaaccaatat caacgcggaa atccagcacc
    19921 gcttgcatca cgtcctggct gttcccaacg ctaagttcaa tcggcaactg cggataatca
    19981 tggcgataac gggcgataac tgcaggcaga atgtagttac cgatggtact actggcatag
    20041 atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc ctgttcaagc
    20101 aatgccaacg cacgcggata gagcagccgc ccgtgttcat taacaaccag tcttttcccc
    20161 acgcgatcaa acagttgcac gccaagctgc ccttccaggt cggtcaaggc tgcgctcact
    20221 gctgattgcg acaacgccag catcaccgac gcctgggtgg ttgatccact tttcaatact
    20281 tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgcttacca cttataaaga
    20341 ttaattataa atatataatc aattttattt ttaaaccagt tagtcgtaac cttatgcccg
    20401 gttaaaggag agggttatga cgaatatcac cttacagaaa caacatcgta cactgtggca
    20461 ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc
    20521 cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat
    20581 ggttttaggc aacaccatct atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt
    20641 tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc
    20701 gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgctgacgc tgtccagtac
    20761 cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg
    20821 gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagccggt
    20881 agtgaaagcg gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac
    20941 cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga
    21001 aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg
    21061 tcatgccatc agcccggatg cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat
    21121 gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa
    21181 cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atattgttca tcgtcgttgc
    21241 catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga
    21301 taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct
    21361 gaaaaaagcc ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt
    21421 tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg
    21481 ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca
    21541 tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg
    21601 ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg
    21661 ccgcaccgct cacgacgcaa accatcgatg aattcaaagc ggcctgtgaa aaatatcact
    21721 acacatcggc gcaaattctt ccccacgaca gctatctgat taacctcgga catccggtca
    21781 ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac
    21841 tggggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg
    21901 attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtaa
    21961 cagcggtgat tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc
    22021 tcgcggcgat tatcgacggc gtggaagata aatcccgtgt cggcgtctgc attgatacct
    22081 gccatgcttt cgctgccggg tatgatttgc gtactccagc cgaatgcgag aaaacgttcg
    22141 cggattttgc ccgtattgtt ggctttaagt atctgcgcgg gatgcacctt aacgatgcga
    22201 aaagcacctt tggcagccgc gttgaccgcc atcatagcct cggtgaaggc aatatcggtc
    22261 atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg ctgatccttg
    22321 aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg
    22381 aaaaagcggt ggcctgatcg atgaacgaca gggaaaagca gattcttaaa attttacggc
    22441 gtaacccgct gattcaacag aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg
    22501 ttgccgctca tattatggat ttgatgcgca agggtttgat taaaggcaaa ggatatatcc
    22561 ttaccgagca ggattactgt gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg
    22621 cggatatccg ttacccgcaa gcagcctctc atccaggttc tgttcactgt tccgcaggcg
    22681 gcgtcggacg taatatcgcg cataacctgg ctctgcttgg tcgggatgtg catttaatat
    22741 cagctattgg caacgatttt tacggcgaga cattactgga agaaacgcgc cgcgcgggcg
    22801 tgaacgtttc aaactgcatt cgtctgcatg gacacagcac tgcaacttat ctcgctatcg
    22861 ctaataagca ggaagaaact atcctggcaa tcaatgacac acatattctg caacagctaa
    22921 cgcctcaact gctcaatact tcacgggact tgattcgcca tgctggtgtg gtgctggctg
    22981 attgtaatct cacacctgaa gccctggagt gggtctttac cattgctgat gaaattccga
    23041 tgtttgtcga taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg tacagccgga
    23101 tccacactct gaaaccaaca caaaatgaac tggaaatttt gtgggggcaa ccgattaaag
    23161 acgacaacga tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt aaacgcattt
    23221 ttgtttatct gaaagatgaa tctgtatttt gcagcgataa ggatggagaa caatttttgc
    23281 tgaccgcgcc agcgcatacg acagtagaca gttttggtgc tgacgatggt tttatggcgg
    23341 gcctggtata tagctttctt gaaggataca gtttccgcga cagcgcccgt tttgcggtag
    23401 cctgcgcggc aatttcgcgc gccagcggca gcttaaacaa ccctaccctg tctgccgata
    23461 acgcgctttc attagtgcca atggtgtaac aatgttgccg gattcgacgc ttgaccgcat
    23521 cttatccggc tcataaattg cgcactacgc taaaccaata aagaatcctg caatagtagc
    23581 actcatcagg ttagaaagtg tcgccgccgc cagcgcgcgt aaaccaagtt gggcgatttc
    23641 cggcgcacgg tgtggcgcaa ccgcagaaaa cgcccccacc accaccccga tagaaccaaa
    23701 gttagcgaaa ccgcacaacg cgaaagaaat aatcgccacg gttttagcat ccagagtgcc
    23761 acccgtttgc agatatggcg agaaattgag ataagcgaca aattcattga tcgccagctt
    23821 ctgcccaatc aaactcccgg caagatttgc atcactccag tcaaccccca tcacccacgc
    23881 caatggggcc aacaggtaac ctaaaatgga ctccagcgag gcatgtgcaa aaccaaacca
    23941 gccgccaacg ccgccgataa taccattaat taacgcaatg atggcgacaa atgccataac
    24001 aactgtcgct acaccggcgg cgattttcag cccggtcatt gcccctgtcg cagcggcttc
    24061 aataatgctt tttggcggtg tttcggtgaa agagaggtta ttaaaggaaa cctgcgaaga
    24121 ttccgtagcc gggcttaaca ggcgggcaaa caagatcccg cctgggatcg ccattaacga
    24181 tgccgccagc aaatattcca caggtacgcc cagggcggcg taaccaatca ttgtcgaacc
    24241 agcaatcgag gccatgccac tacaaatcgc tgtaaataat tcattgcgat tcagacgatc
    24301 gataaagggt ttgacgatcg ccggaatttc gttttgcccg aggaaaatgg tggtgaccgc
    24361 gacgaatgac tcaatcttgc tgatatttaa tgctttctgg aatataccgc cgagaatgcg
    24421 aattaaaatc cccatcacac cgatgtagta gagaatactc accagtgcag tgacgaagat
    24481 aattgccggt aataccctga aaccaaagat aaatcctgcg ccatcaaaca gcgtgtccat
    24541 tttcggcccg accagcgaac cgaagataaa tgcgctaccc gcgtcgctgt acgccatcac
    24601 tttatgcacg ccaaaagcga ctttttcagc gacccaacgc cctggcggta accaaagcat
    24661 aatcccgcca atcacaacct gtaacactaa cgccgcgcca acggtacgca ggctgatctt
    24721 cttcttgttt actgacagca aaaatgcaat tgccagcaat accaccattc ccagaacact
    24781 tctcatgaca tccatagtga ttatctcttc atgtcatatt gggcgacggc tttctgccca
    24841 tcagaaatag ccgggcctgc atcttgcgct atcggcccgg cttgttatca acgcttaatg
    24901 ggttttgatg tagccgcgca cgcactcttc gactaagccc cagaaccagt cgatatcaat
    24961 agtgatgccg actttggtat tggcgggctt gccaagaacg cccagctcgt cgcagacggt
    25021 gcgcccatag caagggccac tgttgacgtc cacttcgacg tacatctcct gggttttaat
    25081 gccatcaggg ttaatcagat aaccgatgca ggtggcgtcg tgcaccgggc cgccagccag
    25141 gccgtagttt tcgaactgcg ttttgagagt gaagttcatg atgtcgctga acagctctcc
    25201 ggcagggccg cctgcccttt ccatccgagc aatcacgtcc ggggtgcaaa cggtctggtt
    25261 ggtgagatcg aggcccatca tcactaatgg aacgccagag gtgaacacca cgcgggctgc
    25321 ttccgggtca gcaaagatgt tgaactcggc agatggcgtg aagttgcctg taccgtaagc
    25381 gccgcccatt agcacaattt cacggatttt gggcaggatc gcgggttgca tacgcattgc
    25441 caccgcgata tttgaaagcg gaccaaccgg caccagagtg atatcgccat cgcttgccat
    25501 cagggtatca atgatgtatt tcaccgcgtg ggtactttct gcctggcggg tcagcggctc
    25561 gaataccggg ccatccagtc cggtttcacc gtgaatatta tcggcaacga tttgttgacg
    25621 cataatgggt tgcggcatcc ccgcataaac cggaacatta atctccagtt tctggcaaac
    25681 attcaggcca ttaattaatg ttttatcaag cgtctgatta cccgctacaa tagtgatgcc
    25741 taataaatct attgccggat gtttcgctgc catcatcatt gcaatagcat catcatgacc
    25801 cggatcacaa tccagaataa tttttctctt ttccattgtt tatttcctct gtttccagtt
    25861 gcgttatttt ttctacagca aagaaaattc gaccgggccg atgattgaat cttaccaacc
    25921 gcgtacgtat gctaaatatg agaaatctca tagcggataa acatcgtgaa agaaatccac
    25981 aataatgatc ttaagcagca attgatgagt gaatccgcgt ttaaggattg ctttttaacg
    26041 gatgtttcag ccgatacgcg gctgtttcat tttttagcgc gtaactacat tgtgcaggaa
    26101 gggcaacagc cgtcctggct gttttacctg acgcgaggcc gcgccaggct ttacgccacg
    26161 ctagctaatg gtcgcgtgtc gctgatcgat ttctttgccg ccccctgttt tattggcgag
    26221 attgagttaa tcgacaaaga ccatgaaccg cgtgcggtgc aggctattga agagtgttgg
    26281 tgccttgcgc tccctatgaa acattaccgc ccgcggttat taaacgacac gctattttta
    26341 cgaaaactct gcgtcacctt aagtcataaa aattatcgta atattgtttc tttaactcag
    26401 aatcaatcat ttccgttagt gaatcgcctg gcagcattta tattactctc gcaggaaggt
    26461 gatctttatc acgaaaagca tacgcaagcg gcagagtatt taggcgtttc ttatcgacat
    26521 cttttatatg ttctcgcgca attcattcac gacggtttat taactaaaag caagaaaggg
    26581 tatctcatta aaaacagaaa gcagttgtca ggactggcgc tggagatgga cccggagaat
    26641 aaattctccg ggatgatgca gtaaaaatta tttgcaatag cgcgattgcc ggatgcaacg
    26701 cttaatacgt tttatccggt ctacaaatcg agcattacgc cagaccaata aagaacccgg
    26761 caatagtcgc actcatcagg ttggaaagcg ttgctgctgc cagcgcccgt aaaccaagct
    26821 gggcgatttc cggcgcgcgt tttggcgaaa tagccgaaaa tgcgccaacg acaacaccga
    26881 tagaaccaaa gttagcaaaa ccacaaagcg caaaggagat aatcgcaatg gttttcactt
    26941 ccagcgtgcc gcccgtttgc aggtatgggg agaaactcag gtaagcgacg aattcgttaa
    27001 tcgccagttt ctgcccaatc aggctacccg caagattggc atcactccag tccacaccca
    27061 tgatccacgc cagcggtgcc agcacatagc caaaaatact ttccagagag gcattggcga
    27121 aaccaaacca gccaccaatt ccgccgataa tgccgttgat cagcgcaata attgcgacaa
    27181 acgccattac caccgtcgct acaccagcgg cgattttcag cccggtcatc gcaccgctcg
    27241 ccgccgcttc gataaagctt tttggcggcg tttcgctgaa cgacagattt tcaaatgtga
    27301 cctgtgaagg ctcggtggcc gggctaagaa tacgtgcaaa caaaatacca cctgggatcg
    27361 ccatcagcga tgccgctaac aggtagtcaa ttggtacgcc cattccggca taaccaatca
    27421 tcatcgaacc agcaatggac gccatcccgc tacaaattgc ggtaaacaac tcgttgcgat
    27481 tcatgcgatc gataaacggt ttaacgatcg ccgggatctc attttgcccg aggaaaatag
    27541 ttgtgactgc gacaaaagat tcgattttgc tgatgttaag agctttctgg aaaatgctgc
    27601 caaggatgcg aatcagcagc cccatcacgc caatgtagta cagcagactg atgagcgcag
    27661 taacgaaaat aatcgccgga agtacgcgaa aggcgaagat aaaacccgca ccgtcaaaca
    27721 ggacatccat tttcgaccca accagcgaac caaaaatgaa ggcgctaccg gcatcactgt
    27781 aagacatcac tttatgaacg cctaatgccg cctgttctac tgcccacttt cccggtggga
    27841 agtagagcat gatgccacca atagcgattt gcagcagcag tgcggctcca accgtgcgca
    27901 aactgatgct ctttttattc actgacaaca gaaatgctat tgccagtaac accaccatcc
    27961 ccacaacact tctcattata tccataatga ttttcccttc atgccagtaa acccggcgtc
    28021 agcgccaggt tttggtatgc ttgatgagta cgggcgacgg ctttctgccc gtcagaaatt
    28081 aacccgcgag acgctggtat tctttggcaa tttcactcgc cagaatggcg ttgttgaaca
    28141 ccagctggat attggatttc aggctgtcac cgccggtcag ctcagcaacg cgcgccagca
    28201 ggaacggtgt actttcttta ccaataacgc cctgctcttc ggcttcggcg acggcttgat
    28261 ctatggctgc attgatagat tcctccggca tcgcaaactg ttccgggatc gggttcgcta
    28321 ccacgaggcc accgttcagc ccgctttgcc atttcaccgc cattgcacgg gcaatttcac
    28381 tggcgctgtc gagacgaatg ctgacgtcaa acgagctggt acggcagaaa aacgcaggca
    28441 gcgctttagt ctgatagcca attaacggca caccgaaggt ttctaaatac tcagtggtta
    28501 atcctaaatc gagaatagat ttcgccccgg cacaaacaac ggtgacatta gtatttgcca
    28561 gttcttgcaa atcggcagaa atatcgaagg tatgttccgc cccgcgatgc acaccaccaa
    28621 ttcccccggt ggcaaatact ttaattccgg caagcgccgc aataatcatc gttgaagcca
    28681 cagtggttgc gccatttttt ccggcggcaa caacaaaagg taaatcgcga cgactaactt
    28741 tggtcacgtt atgcccttca cgacccagta attcaatttc ttctttgctt aaacccactt
    28801 tcatcacgcc gccaataatg gcgatagtgg caggtactgc gccctgttta cgaatagttt
    28861 cttctacttc aattgcggtc tgggcatttt gtgggaacgg catcccgtga gaaataatgg
    28921 tcgattccag cgccacaacc ggttttttgt tttttaaagc gtcctgcact tccggggaaa
    28981 tttgtaataa ttcaggggaa atttttaatt cagacattct gcgttctcca ctaacgatat
    29041 aacgttggca atcgataaat cggggttatt ggtgtattca caggagagcg ccattgacga
    29101 acatccctgt gcgaaacgaa cagattcggc aaacggcatt ccgtctaccc aacacgaagc
    29161 aagtcccgcc atcatggcat cgcccgctcc ggtaacatta ataacattgg ttttgatcgg
    29221 cgcagaccag ccattttcac cgctgatatc gctgtaataa acgccatcgc cgcccatgct
    29281 caataccagt cggttcaggc catgttgatg gaaccaggca gcaacttttg ccacatcgtc
    29341 acgccctgac agcgcaatcc cactcagggt ttccgcttca aggcggtttg gcttgagagt
    29401 gtggatctga tttagacggt cgcgcacttt gacacatttc cacgctgaaa ccggatcgac
    29461 aaatacgggt acgttggcgg cattatccag aatccatgcc agtgcctctt cactgatatt
    29521 acagtctgcg acaatgacct ttgccctctg aataaattca cggtgctgtg cgagatattc
    29581 agctgtaata gcgttgctaa tattcatgtc atttatagca accagcattt caccggtatt
    29641 atcgagtaat gataaataac tagacgtatt ttctcccggc acaatcaggc atttatcgac
    29701 ataaacgcca gattgattgg tttgcgttag cagcgattga ccataaaaat cactgcctac
    29761 tgcgctcagt agccaggctt tgttacccag caacgccagg ttttgtgcaa tattgcgccc
    29821 tactccacca ggcgtaaatt ttattttacc tggatttgaa tccgcataat ttaatgattc
    29881 atgtgaatat ccggcgacat caatattcgc cgaacctata attacgacat aatccttttc
    29941 gcgcatagca cgtcctctgg taaaaaatta ccaaccctgt caatccacaa gaatatagat
    30001 actgcaaaga cggtttaaac atgtgttcat atttaaacat gtgctcatag tatgcatgtt
    30061 ttacgtaaag taaattaccg ccagagaggc aaaatgggca gatttgagca ggctcacaaa
    30121 atgcgattaa atgcctgaat tacaaaagcg aggaatttgt gacgggatgc acagaacagg
    30181 aacactaaaa aagggcagaa aataatctgc ccttgaggaa ataaccttag tgtttgatga
    30241 acgtttcagg tttctactga aagataaaac catttaaatt cataaaatta catttacaaa
    30301 ccggtcattc cctttgcgct catccaatga tacagggcag gaaatttctg ccctgtaaca
    30361 caccttttat tacgctgctt tcgctactgc gtccacttcc ggacgtttca ggaaggcata
    30421 ggccaaaccc gccaccagcg taccggcaat aattgctacc aggtaaccca ataccggcgt
    30481 aatagcgcca gggatcagca gaacaaacag accaccgtgc ggtgccatca gtttcgcacc
    30541 aatcgccatt gagattgcgc cagtcagcgc cccacccacg atacagcacg gcagcacacg
    30601 catcggatca cgagcagcaa acggaattgc accttccgaa ataaagcaca gtcccagtac
    30661 cagagcggct ttgccacctt cctgctgcgc tttgtcgaat ttgcgacgcg ccaccattgt
    30721 tgccaggccc attgccagcg gtggcaccat acctgccgcc ataatcgccg ccatcgggcc
    30781 ataggtttga gtactcagca gacccacacc gaatgcgtac gctgctttat ttaccggacc
    30841 gcccatgtca gtacacatca tgccaccgag gatcgccccc agcagaaccg cattcgcagt
    30901 ccccatggtc tgcagccagt gagtcaaccc ttcgagaatg ccagcaaccg gtttaccgat
    30961 caggtagatc atcgccagac cgaccaccag actggaaatt agcggaatga tcaggatcgg
    31021 tttcagcgcc tccatactct gtggcagttt caattgcgta ctgattaact tcgcaatgta
    31081 accagccagg aagcccgcaa taataccacc aatgaagcca gaaccggtgc tgaccgccag
    31141 cataccgcca atcagacccg gcgtgaggcc cggacgatcg gcaatggaaa aggcaatata
    31201 acctgccagt accggcacca tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc
    31261 cgctgccaac gtacccggct ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat
    31321 acacagacca ccagcaacga ccatcggcag catgtaagag acgcccgtta gcaagtgacg
    31381 ataagcgcct gcactctctt tcttaccttc agtggtcgcc gtttgagttt tgcccgccgg
    31441 ttcatacggc gttgcttcag caaccgcttt atccagttcc tgcgcggttt tcttcagcgc
    31501 cagaccggta gaggtacgat acatcggttt accagcaaat ttcgccagat ccacttcgat
    31561 atctgccgcc acaatcacca gatccgctgc ggctacttcc tcgggagtga ttgcattacc
    31621 cgcgccaaca gaaccacggg tttcaacttt cacccaccag ccacgttttt tcgcttcggt
    31681 ttcaatggct tcagccgcca taaaggtgtg tgctacgcca gtcgggcaag cagtcaccgc
    31741 aactacgcgt ttcggaccgc tggcggcaac cggtgctgtc gcagtgaccg gcgcagtgta
    31801 aggtttcgca tggcctttgg cttcactcag gaacagctca gggtgcgcaa ctgcccggga
    31861 aatatcaccc agccagacat ttttaccgtt cagcgcgctg tcattcggga tggaatcacc
    31921 gagaacaatc gccatttctg cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg
    31981 cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt gcctgaccga gattagcgtc
    32041 aataatcagc agcgttttca ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga
    32101 cgcgcgccat cattgcggcc aactgcggac gatcggtaat acccacattg ctttgactta
    32161 ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg ttcactggat tcacgcatca
    32221 gcaagccata aatcaggcca ccaaccatag aatcccctgc gccaacggtg cttacgacat
    32281 cgactgacgg tggtttggcg atccattcgc cggaggcatt aacccaaagc gcgccttcgg
    32341 cacccagtga aataacaaca tgcgcaatac cttgttcacg tagcgcatgt gcagcttcaa
    32401 tcacatcttt catttcaggc agtttacggc ctgcccagat ttccaactcg cggcggttag
    32461 gtttcaccag ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg ctactatcaa
    32521 agataatgca aggacactga ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc
    32581 tgacgcctga cggtaagctt ccgctgacac agaccatatc gaactgaccg agccagctca
    32641 gagaatcagt cacaaagcgt tcccagtcgg cgggggtgac ttcaaaaccc gagaagttga
    32701 agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt aattcgggtg cgcccctgta
    32761 caacctggaa acggttggca atgcccagct cgctgaacag ttgctgaaaa ccatcctgat
    32821 tgtctttacc caggaagccg ccaacggtga catcaattcc caggtctttt aatactttgg
    32881 ccacgttgat gcctttaccc gccgcatgca gaccggtggt tttcaccagg ttcacttcgc
    32941 cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc cggattaagg gtgatagtag
    33001 caacacgtct gctcattatg cgccctcccc aagaccagca gcgatagcgt cgccgattgc
    33061 tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt
    33121 aacgccaagt gccacaactt tcatcagact acgtccgttt gccggtttgc cggtaccatc
    33181 aaggtttgtc acggtaatat cactgttaaa ttgtttaatg gtattgacca gcatggtacc
    33241 tggacgagca tgcaggccgt gttcattgcg caccacaaac tccgcgctta acacgtcgtc
    33301 ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa
    33361 gcggtcagct ttattgtcga gcaacaaatc agcgagacgc ttaagaaccg caatgggctg
    33421 atcgtcattc atcgccacac tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc
    33481 atttgccgca cggcttaccg caatcgcgct acgcagattg ccttcggcgc tatcgctcag
    33541 ccagataccc tgtccgaggt tcagcggttg ttcattgatg gctttggtga cgaaagtggc
    33601 gtcaactgcc ctcgcctctt tcagacgcgc agcgttcagc gcctgaagag tcagcagatc
    33661 gctggcgacg atatccagcg tcagcatttc gttgtcgagc ttcagctgct cactctgctt
    33721 ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt gctgacttca gttgttcagc
    33781 aacggaatca tcgctcagta cgtgggtcag ctggcgtagc aggcccagat gttcatccga
    33841 gctggcagca ataccgattg ccacgtacgc tacctgaccg tcaccccagg tgacgccttc
    33901 cgggaactga aatacctgaa cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc
    33961 gtgtggaata gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat
    34021 gccattgacg tagccttctg ctacattacc ggcctgcacc agcgccgcag cgacctggcg
    34081 aatcgcctct tctttgtctc cggccttttc gcccggatgg atgtcctgta cggataactg
    34141 gaacatagtt ctcctctctt gctgaattga aacgattcag cctctatgag aaaaaaagcg
    34201 ccaacctggc ttagggttaa agacaagatc gcgctgaaac gtttcaagaa agcataatac
    34261 ttctgtttca gcacgcaagg aaagccgtaa agttatgagc aaaagtttga tgtgctgcac
    34321 aattttttgc catttttcct aattgctgat gggaaaatcc gttatcagca atttcatttc
    34381 agcagcgtca gcagccaatc agcaacagtg aaatggcatt ttgattattc gtggcgcttt
    34441 tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa ctccgtgtct tcctcttcca
    34501 gtgatcgacc agcatgcata actcccccgc agtctccagc gcgaaatcgt ttgacctgac
    34561 ctcgacggcg tttttaatcg ttgcctttct caccggtatt gcgggcgctc tgcaaacccc
    34621 gacactcagt atttttctta ctgatgaagt acatgctcgt ccggcgatgg tgggattctt
    34681 ctttaccggc agcgctgtca ttgggattct ggtaagtcag tttctcgccg ggcgctctga
    34741 taagcgcggc gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg tgctggcctg
    34801 cacccttttt gcttggaatc gcaactactt tgttttgcta ttcgttggcg tctttcttag
    34861 cagctttggc tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac atgccgacaa
    34921 aaccggacgt gaggcggtga tgttcagctc ttttttacgc gctcaggttt cactggcatg
    34981 ggtcattggc ccaccgctgg cttatgcctt agcgatgggt ttcagcttta cggtaatgta
    35041 tctgagcgca gcggtagcgt ttattgtttg cggtgtgatg gtgtggctgt ttttaccgtc
    35101 gatgcaaaaa gagcttccgc tggcgaccgg cacgatcgaa gcgccgcgcc gtaaccgtcg
    35161 cgatacgctg ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca gcctgtacat
    35221 catcaacatg ccgctattta ttatcaacga actgcatctt cccgagaaac tggccggtgt
    35281 gatgatgggg accgccgccg ggctggaaat cccgacgatg ttgattgccg gatatttcgc
    35341 caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta
    35401 cgcaggaatg ctgatggcgc attcacctgt cattctgttg ggcttgcagc tgctaaatgc
    35461 tatttttatt ggcattctgg gcggcatcgg gatgctctat tttcaggatc tgatgcccgg
    35521 tcaggcgggt tcagccacca cgctctatac caacacgtcg cgcgtgggct ggatcatcgc
    35581 aggatcagtg gcgggcatcg tcgccgagat ctggaattat cacgctgtgt tctggtttgc
    35641 gatggtgatg attatcgcca ctctgttttg cttactgcgg attaaagatg tttaaggcgc
    35701 ggtcagcatt tccagatcga taagccaggt catggcctgc tgacgcgagt taccgcacat
    35761 ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt
    35821 acagcgttgc tgttcatcaa gctgaatgca gggcgtattg gcgggcttgc catctggcat
    35881 accgggaata gggctggaaa ttgaaggggc agtgcaacac gccccgcaac ccggacggca
    35941 tttcatacgt tgttctctta aggttaacca ccggattgat gagcgcgcac agtaccacct
    36001 tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg
    36061 tcgcgatatt tttgacattt tcgactacag gaatttttcg atgccaagag cgaacgaaat
    36121 taaaaaaggt atggtactga attacaacgg caaactgctg ctggttaagg atattgatat
    36181 tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg
    36241 taccgggctg aaagtagaag agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct
    36301 gacccgccgt tacgttgact tctcctatgt cgatggcaac gaatatgtct ttatggataa
    36361 agaagactat accccgtata ccttcaccaa agatcagatt gaagaagagt tgctgtttat
    36421 gccagagggc ggcatgccgg acatgcaggt gctgacctgg gacggtcaac tgctggcgct
    36481 tgagctgccg cagaccgttg atctggaaat cgttgaaacc gcaccaggca tcaaaggggc
    36541 atctgccagc gcccgtaaca aaccggcgac attgagcact ggtctggtga ttcaggtacc
    36601 agaatactta agcccgggcg aaaaaattcg tatccatatc gaagaacgcc gttatatggg
    36661 gcgtgctgac taacttcagc cgcatgcaga aaagggatag ctcaggctgt ccctttttta
    36721 atttattata ccaatcttct attttgcgct tcctgaacac cttacgccct caattttcac
    36781 tcgttgattg atcgccctca cacttcatcg cattaacaat ccagaccaat ttcaattgct
    36841 gtcatataac tttacactgt cattgttaat ttatcgttac taagacgtga ctcctatgaa
    36901 gacaattgcc tccgttacgc tcccgcatca tgtacacgct ccacgctacg atcgccagca
    36961 gttgcaatca cgtatcgttc attttggctt tggcgccttt caccgcgctc atcaggcgtt
    37021 actgaccgat cgtgtgctga atgcccaggg cggcgactgg gggatctgtg aaatcagctt
    37081 gttcagcggt gatcaactga tgagccagct ccgcgcacag aaccatttat ataccgtgct
    37141 ggaaaaaggt gcggacggca atcaggtgat aattgtcggt gccgttcacg aatgccttaa
    37201 tgcaaaactg gattccttag cggcaattat tgagaaattt tgcgagccac aggtggcaat
    37261 tgtttccctg acgattaccg aaaaaggcta ttgtattgac ccggccaccg gtgcactcga
    37321 caccagtaat ccgcggatta ttcacgatct acaaacccct gaagaacctc actccgcacc
    37381 gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc ggccttacac cgtttaccgt
    37441 gctctcctgc gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat
    37501 ggcagaaaaa cgttcgccag aactcgccgg gtggataaaa gaacacgtca gttttccggg
    37561 aaccatggtc gaccgcattg ttccggctgc aaccgacgaa tcactggcag aaatcagcca
    37621 gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt
    37681 ggaagataac ttcgtcgctg ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa
    37741 tgatgtcctg ccatgggaag agatgaaact gcgtatgctt aatggcagcc actcttttct
    37801 cgcttatctg ggttaccttt caggattcgc ccatatcagt gattgcatgc aggatcgcgc
    37861 atttcgccat gccgccagaa cattaatgct gaatgagcaa gcgccgacac tacaaattaa
    37921 agatgtcgat ttaacacaat atgcggacaa gttaattgca cgttttgcta atccggcgct
    37981 gaaacataag acctggcaaa tcgcaatgga tggtagccag aaattaccgc aacgtatgct
    38041 ggcaggtatt cgcatacatc tggggtgcga aacggactgg tcgttgctgg cattaggcgt
    38101 tgctggctgg atgcgttacg tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg
    38161 cgatccgctt agcgataaaa ttcgcgaact tgttgcgggc agcagcagtg aacaacgcgt
    38221 aaccgccctg ctttccctgc gtgaagtttt cggtgatgat ctgccagata acccgcattt
    38281 tgtgcaggcc atcgaacaag cctggcaaca aatcgtacaa ttcggcgcac atcaggcgct
    38341 attaaacacc ctcaaaattt aacgatttct gcggttaaag cggatgaagc tcaccttcgt
    38401 ccgctctccc cttctctttt ctgccttttt tagccaggat taacgctcag ttaacttacc
    38461 agagtattgc ggagccgttg tgaccaggac caacctcatc accggttttc tcggcagcgg
    38521 gaaaaccacg tcgattcttc atctgttagc ccataaagat cccaacgaaa aatgggcggt
    38581 actggttaat gaatttgggg aagtcggaat tgatggtgct ttgctcgccg acagcggcgc
    38641 attgctgaaa gagatccccg gcggctgcat gtgctgcgtt aatggtttac ccatgcaggt
    38701 agggttgaat accttactgc gtcagggaaa accagaccgc ttgttgatag agccgaccgg
    38761 gctgggccat ccgaaacaga tcctcgatct cttaaccgca ccagtctatg aaccgtggat
    38821 agatctgcgc gccacgttgt gcattctcga tccacgcctg ctgctggacg aaaaaagcgc
    38881 cagcaatgaa aacttccgtg accagctggc tgccgcagac atcattgtcg ccaataaatc
    38941 cgaccgtgcg acacctgaaa gcgagcaagc gctacagcgc tggtggcagc aaaatggtgg
    39001 cgatcgacaa ttaattcaca gtgagcatgg gaaagttgac ggtcatcttc tggatttgcc
    39061 gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat tctcatcagc atgtcgtgaa
    39121 aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag
    39181 cgggcaagga tatcaggcct gcggctggat attcgacgct gatacggtat tcgacaccat
    39241 tggcattctg gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat
    39301 tcccgaaggg ctggtacgaa tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa
    39361 cgttgcgcca ccggacagcc gtattgagct gatttccagc agcgaagctg actggaatgc
    39421 tttgcagagc gcgctgttga agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt
    39481 ttcagtaaga taattagaga aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat
    39541 attgtcggcc tcgcgctgtt tctttcctgg tatattcccg ttaatcatgg attctggttg
    39601 ccaattgatg cggatatttt ttatttcttt aatcagaaac tggtcgaaag taaggccttt
    39661 ttgtggctgg ttgcattgac caacaatcgc gctttcgacg gttgttctct gctggcgatg
    39721 ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc ctggcagacg acgtatcgtg
    39781 attatgggtc tggtcatgct attaactgca gtggtattaa accagctggg tcaggcatta
    39841 attcctgtaa aacgggccag cccaacattg acttttaccg atattaaccg cgtcagcgaa
    39901 ctgctctctg ttcccacgaa agatgcctca cgagatagct ttcccggcga tcacggcatg
    39961 atgctgctta ttttttcggc attcatgtgg cgttatttcg gcaaagttgc aggccttatc
    40021 gcccttatta tttttgtggt ttttgcattt cccagagtaa tgattggcgc acactggttt
    40081 actgacatca ttgtcggttc gatgaccgtg atattgatcg gtttgccctg ggtgttgctg
    40141 acgccattaa gtgatcgatt aatcaccttt tttgacaaat cactaccggg aaaaaacaaa
    40201 catttccaaa acaaataact cacagtaatt aacatcatca ggcttatttt tatagtgagg
    40261 ataatcctga tgatgcgcac cgtgctttca tccatcgcgc gcaaaaatca ttctctaagt
    40321 aaatgaatgg attgcatgcg tttcactcaa tcgtacttta attgaccaat cccgcttatt
    40381 aactttctgt atcacttttt cttataaaaa atcatgtaaa accgctcgcc aagaccgcac
    40441 caatcgggta atctcgaact cgttttgcct cggcggtaga ttatcctcac agcatataat
    40501 tttgtgcgtt agtccacaga tttggcctta aggaattgtt tcaacatgcc caggtaatta
    40561 gtctcgtgtc gcttggcatt tttttataac gatatttgtc gttaaggact tcaagggaaa
    40621 acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg gatccccgcg
    40681 attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat
    40741 cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca ggatgaattt
    40801 gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg
    40861 aaaggcgtac gttatcgtct gggcggcagc actaaaaaag gtatcgattg ttctggtttc
    40921 gtacagcgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac ttacgaacag
    40981 caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt agttctgttc
    41041 cgtgccggtt caacgggacg ccatgtcggt atttatatcg gcaacaatca gtttgtccat
    41101 gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccgtactg gaagaagcgt
    41161 tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat gcaatccctt
    41221 ggctatccta acgagttaac tgaaagcact gcttaggcag tgcttttttg ttttcattca
    41281 tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat tgttatcttt
    41341 taaatgttgt cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc tcccaattct
    41401 ggacgtaagc tcctgcttac ctgcattgtt gcaggcgtga tgattgcgat actggtgagt
    41461 tgccttcagt ttttagtggc ctggcataag cacgaagtca aatacgacac actgattacc
    41521 gacgtacaaa agtatctcga tacctatttt gccgacctga aatccactac tgaccggctc
    41581 cagccgctga ccttagatac ctgccagcag gctaaccccg aactgaccgc ccgcgcggcg
    41641 tttagcatga atgtccgtac gtttgtgctg gtgaaagata aaaaaacatt ctgttcatct
    41701 gcgaccggtg agatggacat tccactcaat gaattgattc cggcgctcga cattaataaa
    41761 aatgtcgata tggcgatctt acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc
    41821 atctggtatc gcaacccttt gctgaaaaat agcggcgtct ttgccgctct gaatctcaac
    41881 ctgacgcctt cactctttta tagttcacgg caggaagatt acgatggcgt cgccctcatt
    41941 attggcaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa cgaattaacc
    42001 gacatgccag tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg gctttatgca
    42061 gatgactgga catggaacga tgtgtggtac gcatttttac tgggcggcat gagtggaact
    42121 gtcgttggcc tgctctgcta ttacctgatg agcgtacgta tgcgccccgg cagagaaatc
    42181 atgaccgcca tcaagcgcga acaattttac gtggcgtatc aaccggtggt ggatacacaa
    42241 gctttgcgag taacgggcct ggaagtactg ctacgctggc ggcatcctgt cgcgggagaa
    42301 attcccccgg atgccttcat taactttgcc gaatcgcaaa agatgattgt gccgctgact
    42361 cagcatctgt ttgagttaat tgcccgcgat gccgcagaat tagaaaaagt gctgccggta
    42421 ggcgtcaaat ttggcattaa cattgcgccg gaccatctgc acagcgaaag ctttaaagca
    42481 gatatccaga aactgctcac ttccctaccc gcacaccatt tccagattgt gctggaaatt
    42541 accgagcgcg atatgctgaa agagcaagaa gccacacaac tcttcgcctg gctgcactcg
    42601 gtaggcgtag aaattgctat tgatgacttc ggcaccgggc acagcgcgct tatctatctt
    42661 gagcgtttta cgctcgatta tctgaaaatt gaccgtggat ttatcaacgc catcggtacg
    42721 gaaacgatca cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg cctcaatatg
    42781 ctgacggttg ctgaaggggt cgaaacgccg gaacaagcgc gatggctaag cgaacgcggc
    42841 gttaatttca tgcaaggcta ctggattagt cgcccgttac cgctggacga ttttgttcgc
    42901 tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct tattattcat
    42961 agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccgtt gcagatgatt
    43021 gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgcgcaggc gcaggctatc
    43081 aaggaaagct atgcctttgc cgtgctgggc gaaccccggt acgcgtttaa tttcaaccat
    43141 tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga taacgttgtc agccctcggt
    43201 accttcgata atttcaaccg ctatgcactg cgcggcaacc cgggcgcacg caccgagcag
    43261 ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta cccgctgatt
    43321 gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat taatccgcgc
    43381 gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac ttttcaaaaa
    43441 tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc
    43501 attgcgccgt taaccgtgcg cattgagtta gctaaacccg gcaaagaaga tatgttgagt
    43561 ctgttttcgc tgccggtatt tccagaaaag tactggaagg atcacaaact tagcgatccg
    43621 ctcgccacgc ctccgcttgc cagtggtccg taccgaatta cgtcctggaa aatggggcaa
    43681 aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt aaaccgtgga
    43741 cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt cgcctttgaa
    43801 gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg acgctaaaaa ctgggccacg
    43861 cgttataccg gtaaaaattt cgataaaaaa tacatcatca aagatgagca aaagaacgaa
    43921 tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccggtatt cagcgatcgc
    43981 cgggtgcggg aagcaatcac cctcgccttt gactttgaat ggatgaacaa ggcgttgttt
    44041 tacaatgcct ggagtcgcac gaacagttat tttcagaata ccgaatacgc ggccagaaat
    44101 taccccgacg ccgcggagct ggtgcttctg gcaccaatga aaaaagatct accgtcagaa
    44161 gtcttcacac aaatctacca gccgccggta tctaaaggcg atggctacga tcgtgacaac
    44221 ctgttaaaag ccgacaaact tctcaacgaa gcgggctggg tgctgaaggg tcagcaacgc
    44281 gttaatgcca caacgggtca gccactcagc tttgaattat tgcttcccgc aagcagcaat
    44341 agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa catggacatt
    44401 cgcaaggtgg ataactcgca aatcaccaac cgcatgcgca gtcgcgacta tgacatgatg
    44461 ccgcgcgtat ggcgggcgat gccgtggccc agttccgatt tacagatttc ctggtcatcg
    44521 gaatatatca attccactta taatgccccc ggcgtgcaaa gtccggttat cgactcgctg
    44581 atcaatcaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt ggggcgagca
    44641 ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat ggcggaagac
    44701 cgtctcgcct ggtgggataa attctcccag ccggccgtgc gccccatcta tagcctcggt
    44761 atcgatacct ggtggtatga cgtcaataaa gcggccaaac tgccgtccgc cagcaaacag
    44821 ggagagtaga tgggcgctta cctgattcgc cgtctgttgc tggtgatccc aacattatgg
    44881 gcgattatca ccatcaactt tttcatcgtg caaattgcgc ctggcggtcc ggtcgaccag
    44941 gccatcgccg ccattgagtt tggtaatgcc ggagtattac ccggcgcagg cggtgaaggt
    45001 gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca gcgacagtaa ttaccgtggc
    45061 ggacgcggat tagacccaga agtgatcgct gagatcactc atcgctacgg tttcgataag
    45121 ccgatccacg aacgttactt caaaatgctc tgggactaca tccgctttga ttttggtgat
    45181 agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc ggtttccatc
    45241 accctcggat tgtggagcac gctgattatc tatctggtgt cgattccgtt aggcattcgc
    45301 aaagctgttt ataatggggg ccgctttgac gtctggagta gcgcatttat catcatcggc
    45361 tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc gggcggcagc
    45421 tatttcgacc tgttccctct acgcggcctg gtttccgcta actttgattc gctgccgtgg
    45481 tatcagaaaa tcaccgatta tctgtggcat atcacgctgc cggtgctggc gacagtgatt
    45541 ggtggctttg cggcgctgac catgctgacc aaaaactcat tccttgatga agtgcgcaag
    45601 caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaaa aaaatattct ctggaaacat
    45661 gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt tataagcatg
    45721 ttttttaccg gctcgctgct gattgaagtg atgttttcac tcaatggcct tggcttactg
    45781 ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt gtatattttc
    45841 accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct ggtcgatccg
    45901 cgtatagatt ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag gcccgttggg
    45961 cgcgttttcg ccataaccgt cgcggctact ggtcgttatg gattttcctc gttttgtttg
    46021 gtttgagttt gtgttctgaa cttatcgcca acgataaacc gttgctggta cgttatgacg
    46081 gcagttggta tttcccgttg ttgaaaaact acagcgaaag cgattttggc ggcccgctgg
    46141 caagtcaggc tgattatcag gacccgtggc tgaaacaacg gctggaaaat aacggctggg
    46201 tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc gataagccct
    46261 tcccttctcc accctcccgg caaaactggc tgggaacgga tgccaacggc ggcgatgtgc
    46321 tggcacgcat tctctatggc acgcggatct cggttctgtt tggcctgatg ctgactctct
    46381 gttccagcgt gatgggcgtg ctggcggggg cgctacaagg gtattacggc ggtaaagtcg
    46441 atctctgggg acaacgcttt attgaagtat ggtcggggat gccgacgctg tttttgatta
    46501 ttttactttc cagcgtcgta cagcctaact tctggtggct gctggcaatt actgtcttgt
    46561 ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtact cgtaatttcg
    46621 actacattcg tgcggcacag gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata
    46681 tgttgcctaa tgccatggtc gcgaccctca cctttttacc gtttatttta tgtagttcga
    46741 taaccaccct gacctcgctc gatttcctcg gcttcggtct accgctcggt tcaccgtcac
    46801 tcggtgaact gctgttacaa gggaaaaata accttcaggc cccgtggctt gggatcaccg
    46861 ccttcttgtc ggtggcgata ttattgtctt tgctgatctt tattggtgaa gccgtccgcg
    46921 acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat
    46981 ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag tagtcaatga tgtttcacta
    47041 cagattgagg ctggcgaaac gctggcgctg gtgggtgagt caggttcagg caaaagcgtt
    47101 accgcgctgt caattttacg cctgctccct tccccgccgg ttgaatatct ctccggcgat
    47161 attcgttttc atggcgaatc gctgcttcat gtcagcgatc aaacgttgcg cggtgtacgc
    47221 ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt cgttaaatcc attgcatacc
    47281 ctggaaaaac agctttatga agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct
    47341 cgtggcgaaa ttcttaactg ccttgatcgc gttggtatcc gccaggcggc aaaacggctg
    47401 acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat tgcgatggcg
    47461 ctgttaacgc gaccggaatt attaattgcc gatgaaccga ccaccgcact ggacgtctct
    47521 gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagctgaa tatgggcatg
    47581 ctgtttatta ctcataacct cagcattgtc agaaaactgg cccaccgcgt ggcggtaatg
    47641 caaaacggtc gctgtgtcga gcaaaattac gccgctacgc tatttgcatc acccactcat
    47701 ccttacacac aaaagctact caacagtgaa ccgtcaggcg ctccagtgcc gttgccagaa
    47761 cctgcctcaa cgttgctgga tgttgaacag cttcaggttg ccttccccat tcgcaaaggg
    47821 attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt tacgctacga
    47881 gcgggtgaaa cactgggttt agtgggcgag tccggttccg ggaaaagtac gacgggactg
    47941 gcgctgctgc gactgattaa ttctcagggc agcatcatct ttgacggtca gccactgcaa
    48001 aatttaaatc gccgccagct gttacctatt cgtcatcgca ttcaggtggt atttcaggat
    48061 ccaaactcct cgctcaaccc acgactcaac gttttgcaga ttattgagga aggcttacgg
    48121 gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat
    48181 gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc tggtggtcag
    48241 cgacaacgta ttgcgattgc cagggcatta attcttaagc cctcgctgat catacttgat
    48301 gaaccgacat catcactcga caaaacggta caggcgcaaa tattgacgct attgaaatca
    48361 ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca cgttgtccgc
    48421 gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca aggaccgtgc
    48481 gcgcgcgtgt ttgccacacc gcagcaggag tatacgcgtc agctactggc gttgagctga
    48541 cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa
    48601 tcggcatgtt gcagcaaact cgtcctggcg atttacaaac aggcacggct caccttcaca
    48661 ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt tatacatcac
    48721 cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc tgttgtcacc
    48781 gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag cagaccagcg
    48841 atagttctgc ggcagtcgat ggacgattga gagttgtaat gaagtcactt attttccccg
    48901 gaagtacatt cacttcacaa tttgtttaca tcaattttaa cacatcatca acaatccgtc
    48961 tttatacaga tcgtaaagat tgatgcctcg cctgcgtggc ctctatggct ctgatttaag
    49021 taatatctgc gggcgtggtc acgtttgcgg ggctatatgt acccgtttct gcgatctaac
    49081 tcaacctttt taactacaat gatgtgactt tttacataaa ttgattttac ataaaataaa
    49141 catatatcgg ggggaatgat agatttgtgg ttgacatgca tcaacaaagg aagcctttta
    49201 gcttcctcgt tgtgcaatag atcaccgttt tttcggtcga ctggcgtaca gacagaagag
    49261 aatggagctg cttgcgcaga atgcaattga ccaaatcatc ggccatgcag agttaaaggt
    49321 cgcaagagaa agcaatgcgc caacaattgc ccctatgcca aaacggaagg ttcctgccag
    49381 cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga atgaccgcca tcgcattgga
    49441 tgacaccatc gacacgcagc ccacaaacgc cgcaacgcca accaccagcg accagaatcc
    49501 cagccccagc agcgcactga tgaccatcca cgctgccata ataaattgta tccacaaccc
    49561 cgagcggaac atatttaacg cgccaatgcg gcgaacgaag cggctgttaa aaatggtcat
    49621 cacgaacagg aaaacaatgt ttagtgcaaa gtaataacca aagttttccg gcgcgacgtg
    49681 gttaatttca atgtagacaa acggtccggc gcttaagaac gagaacattc cggcaaagct
    49741 gaaaccgctg gccagcatat aactcaggac acgcttatga cggaacagcg ccgcaaagtt
    49801 accaatagtg gtacgaatgt gaaatggctg acgacgctcc ggtggtaagg tttctttaat
    49861 caggaagaaa atcattgccg aagccagaat cgccgctaat gccaggatcc agaagatgta
    49921 atgccagctc agccacacca gcacccaacc gccgacaatc ggtgccatca acggtgcaat
    49981 agtggtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttcg ggtaaatatc
    50041 gcgcatcaaa gcgttgataa ccacgctggc cgcagccgcc gccagcccgt ggaagaaacg
    50101 catcacaatc agctgatcga tggtgtttgc caacgcacac gccaccgcgg cggcggcaaa
    50161 caccagcgta ccgccgagca ccaccggctt acgcccgaag ctgtctgcca tcggcccgta
    50221 gattaactgc cccaacgcaa agcccagaat ataagtactg agggtcatct gcgtactgcc
    50281 cgcaggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca tatcaatcga
    50341 cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg acgaatgctg
    50401 tcgggtggtc acaacgggct cctgaaagtc atggaaaagt tagacgacgc tggcaatttc
    50461 ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg taataccgcc
    50521 aatacgttca cgatgcagct caaccacgtg gttacccacg gcggcgaaca tgcgtttcac
    50581 ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa tcacttccag
    50641 caccgcaggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag caaattgctc
    50701 tgccgtatcg tcagctacag gtgattccag tgtcaccaga taggtcttct cgcaatgatg
    50761 gcgcggagaa gtaatgcggt gcgaccactg accatcatca gtcatcagca ccagaccggt
    50821 ggtatcgata tccagacgtc ctgcggcgtg cagtttccag gctaccggtt catccagaaa
    50881 atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acatagccct gaggcttatt
    50941 gagcatgaag taacgtggac catgttgctg cgccagcggg ttgccatcgt aagcgacatc
    51001 atgttcagga agcagtttga acgctgcatt gcggacgatt tcgccatcaa cggtgacacg
    51061 attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct gtgcgataaa
    51121 tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaacg gaggtcgggc attgacccga
    51181 aaatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa gggaaaaaga
    51241 tccatggcat actattagca gaataatcta cctacgcgag accatgattt ttacactccg
    51301 cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc ataaaacccc
    51361 tgccgttatc gtgctgccca ccggcgcagg taaaagcctg gtgattgccg aactggcacg
    51421 gctggcgcgt ggtcgtgttc tggtgctggc acacgtcaaa gaactggtgg cgcaaaacca
    51481 tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgctgggt taaagcgcaa
    51541 agagagccac ggtaaagtgg tatttggcag cgtgcagtcg gtcgctcgta atcttgatgc
    51601 ctttcagggt gaattttcgc tgttgattgt cgatgaatgc caccgtattg gtgacgatga
    51661 agagagccag tatcagcaaa tcctcactca cctgactaaa gtgaatcccc acttacgcct
    51721 gctggggctg actgccacgc cttttcgatt gggcaaaggc tggatctacc agttccatta
    51781 tcacggcatg gtacgcggcg atgagaaagc ccttttccgt gactgcattt atgagctgcc
    51841 gctgcgttat atgattaaac acggctatct gacgccgcca gaacgactgg atatgccagt
    51901 agtgcaatac gatttcagcc gcttgcaggc acagagtaac gggctgttca gcgaagctga
    51961 tctcaaccgt gagctgaaaa aacagcaacg cattacgcca catatcatca gccagatcat
    52021 ggagttcgcc gaaaagcgca aaggggtaat gattttcgcc gccaccgttg aacacgcaaa
    52081 agagattgtg ggattactgc ctgccgaaga tgctgcactg attactggcg acacccccgg
    52141 cgctgagcgc gatgtgttaa ttgaagattt taaagcccag cgttttcgct atctggtcaa
    52201 cgtcgcagta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg ccattctgcg
    52261 ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc
    52321 gggcaagact gattgtttaa ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc
    52381 ggaagttggt acaccgaaag gcaaaagtga caacgttccg gtacaggttt tctgccctgc
    52441 ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat tgattgaaca
    52501 ctttggtcgc cgctgtcagg gatggtttga agatgacgac ggtcatcgcg aacagtgtga
    52561 cttccgtttc cgttttaaaa attgcccgca atgtaacgca gaaaatgata ttgccgctcg
    52621 ccgctgccgc gaatgtgaca cggtactggt tgatccggat gatatgttaa aagcggcgct
    52681 acgactgaaa gatgcgctgg tattacgctg tagcggcatg tctttgcagc atgagcacga
    52741 cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga
    52801 gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc gagcagcttt ttatccgccc
    52861 gcatacgcgc acaccgggca tccctctgcg ctggatcacc gccgccgata tcctcgccca
    52921 gcaagcctta ttgcgacacc cggattttgt cgttgcccgc atgaaaggtc agtactggca
    52981 agtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg aattacgcgg
    53041 ttaatggtag ttttgattga tgtataaagc gattgagtat agaatctcgc ccgcttttgc
    53101 atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt aatttaagag
    53161 agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc
    53221 gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa gaagcgccgc
    53281 tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct gaattctaca
    53341 gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct caggacgtac
    53401 agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct taattgctga
    53461 ataagttgta aaaaaccccg ctccggcggg gttttttgta tctgcagatt atgcctgatt
    53521 acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg gcattcagaa
    53581 cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcacg caaattcggc
    53641 ggcgtgcctt taatggtcag ggtatcggtt gccggatccc agaagatgcg ttcacctaac
    53701 agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt cgtcaactga
    53761 cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt cgtagccttt ttcagccgca
    53821 aactccgtga aactcacttc gctgacgcct gccagctctt tcgacagcga ctccagttcg
    53881 atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg gcgcacgttc
    53941 tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc
    54001 ccacggtttt gcgctttcgc gtttaaccct tcgctggccc cgaggaaatc cataaagaaa
    54061 tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt ggactctgga
    54121 ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt gatatcaaga
    54181 taatgggttg gattgatgtc gagattttcg ttaacgcgca tactgctcag gttgctcaga
    54241 actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag cacgaatcca
    54301 ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gcccggttgc cgcgcggcta
    54361 aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc
    54421 tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg cagttctgcc
    54481 accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg
    54541 ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc cagactcatg
    54601 ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg acgcaataaa
    54661 agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca taaaaaagca
    54721 gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg atttcgattt
    54781 atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc agagctgctc
    54841 aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt aggcaacatg
    54901 gtcactaacc ttatcaatac cagcattgcc ccggcccaac gccaggcaat tgccaactct
    54961 tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg gaaacagata
    55021 acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg
    55081 ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca gccgttacct
    55141 gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg taagcattat
    55201 cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc cgctgacctt
    55261 tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa cggcgggaat
    55321 gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc ttaatcccat
    55381 cgtctggcaa ctggttatca acccagacga aaatgagatg gcgcgcgact ggcagctgat
    55441 gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt ggagctggca
    55501 aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt
    55561 tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact tctatcgccc
    55621 gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc gacgttttct
    55681 tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa
    55741 tccagacgcc gtttccgtac agtatccgtt aagcgaactg cgctatcgcg atatgggcac
    55801 cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct tcgagaagca
    55861 gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc atatgagctc
    55921 cggcaacact acagacaacg gcatctttgg cctgttctat ggcatctcgc cgagctatat
    55981 ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta atcagcaagg
    56041 ctatcagctg gggttattct cgtcagatgg ctttaccagc ccgctgtatc gccaggcatt
    56101 gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga ccgccacgca
    56161 gtggatcaac tggctgggcc gctacgcaca agaagataac cgctggttct cgtgggtctc
    56221 tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga aatatagccg
    56281 ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc gtgattctgg
    56341 caaactggac aatacggtgg tgattatcac tgccggtcgg ggtattccgc tgagcgaaga
    56401 cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga ttcactggcc
    56461 aggcacgccg gcgcagcgta ttaatgctct gactgatcat accgatctga tgacgacgct
    56521 gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag gtcaggattt
    56581 gttcaaccct caacgccgtc attactgggt tactgcagcg gataacgata cgctggcaat
    56641 taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca cttacaactt
    56701 acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc aagtgctgac
    56761 agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcaa aatatcttac
    56821 ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt agcgcagcct
    56881 ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg tgccgaccaa
    56941 atcgcccacc ggataccacc ggaattaaca gtttttagac acgaaaagcc cgctaatcct
    57001 tgattggcgg gtttttttat ggtttttagt caccggacat caccggattt ataagctatc
    57061 agccggattt tttagttata cgcttagtta tacgggcttg cgtataacta aagccgtata
    57121 actaaaaccc tgttttttcg tgtggaatca tatacatatg gcgagaaaaa cacccccatt
    57181 aaccacggtg cagatcaaag cagccagacc agcggaaaag gaatacaccc tacaggacgg
    57241 cggagggctt tttctcctgg tcaaaccgtc cggatcaaaa ctctggcgat tttcctacta
    57301 ccgaccatcg gacaaaaaaa gaatattgct gagttttgga tcgcttgatg atgtttccct
    57361 ggctgatgcc agaaaacgcc gtagcgagta cagagcgtta atcagtgccg gaactgaccc
    57421 gcagggccat gagaagaaaa aacgagaggc agaggcccga agacaaggga acacgttcga
    57481 aaatgtggcg gcggcatggt accaggtgaa aatcagccag aatctggccc ccaacacgat
    57541 taaagacatc tggcgatctc tggaaaaata tgtattcccg ttcatcggca acacgccaat
    57601 agacaccctg accgctcgca ggttcgttga ggtgcttacc cccatcaagg agcgcggaaa
    57661 cctggaaaca ctcaagcggg ttttacagcg cgttaatgag gtaatggatt acgccgccaa
    57721 cagtgggctg attgatgcca atccggctat gaatgtgcgt aaggcgttcc cctcccctgt
    57781 aaaaaaacat atgccaacaa tccgccccga acagctgccg gagcttatgc aggctttatc
    57841 agtatcggca acagaacggc agaccagatt actgattgaa tggcagttac tgaccgtaac
    57901 ccgtcccgtt gaagcatcgt cggcacgctg ggaagaaatc gacatggagg cgcaacgctg
    57961 gaccatacca gccggacgca tgaagatgcg ccgcgaccac gttatcccgc tttgtgacca
    58021 ggctatggca gtgctggagg ccatgaaacc aatcagccac caccgcgatt atgttttccc
    58081 gagtctgaaa gacccacggc agccaatgaa cagccagaca gcaaacgcag cattacgacg
    58141 catggggttc gctggcgtgc tggtgtctca tggattacgc gccatattca gtacagcagc
    58201 gaacgaggaa ggattcgagc cggacgtaat cgaggcggca cttgcccacg tcgacaccaa
    58261 cgaagttaga cgggcataca accggagcaa ctacatagaa aaacgcgtgg tgcttatgcg
    58321 ctggtggggc gaatttgtcg aggctgcggc gacgggcgta accctcgcca gtggtaaaag
    58381 gggtatccga gccgtttagc tgtacagaaa accagtaaaa actacgaaaa ccatgtaaaa
    58441 ccgtcgtata attgcatcaa atttaacgac aaggccgtga aacatgaaac cgttaagatg
    58501 caaaaaaatt tcagatgcaa ttgcgacggg ctgcaactgg ccctgatggt tcagcatgaa
    58561 ttttggtcaa cctacgatcc ggaggacaga acgacggccc catcaaaaaa acaggtagta
    58621 gatttcctgg tatcccgtgg ggcatccagg aatatggctg aaagtatcga cagggtagtt
    58681 cgtccggcaa ccatgaagac cggaggcagg ccaaaaaaat ggcggtaaca atcctcaaag
    58741 cggcagaaat gccgcttttt ttataaatcc ttttcaaatc accaacataa aaaacggttc
    58801 taaccgttta aaaacggtgg gaactgtttt tacccatatc cgatgattta ccgtatttgt
    58861 caccggaata caccggattc acaaggtaaa tcacgatgga agcaatcaaa aaagtcatct
    58921 ttcgccagga agtaaaaaaa cttatccaca tcaaggcaga cagcacgctt caaagcatga
    58981 tcaacgccgg agaatttccg cagggttttc gcgttggttt
//
LOCUS       DAZFFI010000015        58514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFFI010000015 DAZFFI010000000
VERSION     DAZFFI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.015"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..882
                     /locus_tag="ACHJ12_001185"
     CDS             211..882
                     /locus_tag="ACHJ12_001185"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO4989007.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            892..2088
                     /gene="yaiP"
                     /locus_tag="ACHJ12_001186"
     CDS             892..2088
                     /gene="yaiP"
                     /locus_tag="ACHJ12_001186"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO4989008.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2066..2647
                     /locus_tag="ACHJ12_001187"
     CDS             2066..2647
                     /locus_tag="ACHJ12_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4989009.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2649..3422
                     /gene="yaiO"
                     /locus_tag="ACHJ12_001188"
     CDS             2649..3422
                     /gene="yaiO"
                     /locus_tag="ACHJ12_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO4989010.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3607..3882
                     /gene="frmR"
                     /locus_tag="ACHJ12_001189"
     CDS             3607..3882
                     /gene="frmR"
                     /locus_tag="ACHJ12_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4989011.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3917..5026
                     /gene="frmA"
                     /locus_tag="ACHJ12_001190"
     CDS             3917..5026
                     /gene="frmA"
                     /locus_tag="ACHJ12_001190"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4989012.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5119..5952
                     /gene="fghA"
                     /locus_tag="ACHJ12_001191"
     CDS             5119..5952
                     /gene="fghA"
                     /locus_tag="ACHJ12_001191"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4989013.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6177..6716)
                     /gene="yaiL"
                     /locus_tag="ACHJ12_001192"
     CDS             complement(6177..6716)
                     /gene="yaiL"
                     /locus_tag="ACHJ12_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4989014.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6818..8029)
                     /gene="mhpT"
                     /locus_tag="ACHJ12_001193"
     CDS             complement(6818..8029)
                     /gene="mhpT"
                     /locus_tag="ACHJ12_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4989015.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8205..9218)
                     /gene="mhpE"
                     /locus_tag="ACHJ12_001194"
     CDS             complement(8205..9218)
                     /gene="mhpE"
                     /locus_tag="ACHJ12_001194"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4989016.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9215..10165)
                     /gene="mhpF"
                     /locus_tag="ACHJ12_001195"
     CDS             complement(9215..10165)
                     /gene="mhpF"
                     /locus_tag="ACHJ12_001195"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4989017.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10162..10971)
                     /gene="mhpD"
                     /locus_tag="ACHJ12_001196"
     CDS             complement(10162..10971)
                     /gene="mhpD"
                     /locus_tag="ACHJ12_001196"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4989018.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10981..11847)
                     /gene="mhpC"
                     /locus_tag="ACHJ12_001197"
     CDS             complement(10981..11847)
                     /gene="mhpC"
                     /locus_tag="ACHJ12_001197"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4989019.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11865..12809)
                     /gene="mhpB"
                     /locus_tag="ACHJ12_001198"
     CDS             complement(11865..12809)
                     /gene="mhpB"
                     /locus_tag="ACHJ12_001198"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4989020.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12811..14475)
                     /gene="mhpA"
                     /locus_tag="ACHJ12_001199"
     CDS             complement(12811..14475)
                     /gene="mhpA"
                     /locus_tag="ACHJ12_001199"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4989021.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14666..15499
                     /gene="mhpR"
                     /locus_tag="ACHJ12_001200"
     CDS             14666..15499
                     /gene="mhpR"
                     /locus_tag="ACHJ12_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4989022.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15576..16658
                     /gene="lacI"
                     /locus_tag="ACHJ12_001201"
     CDS             15576..16658
                     /gene="lacI"
                     /locus_tag="ACHJ12_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4989023.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16781..19855
                     /gene="lacZ"
                     /locus_tag="ACHJ12_001202"
     CDS             16781..19855
                     /gene="lacZ"
                     /locus_tag="ACHJ12_001202"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4989024.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKVAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19907..21160
                     /gene="lacY"
                     /locus_tag="ACHJ12_001203"
     CDS             19907..21160
                     /gene="lacY"
                     /locus_tag="ACHJ12_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4989025.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSIRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21239..22420)
                     /gene="cynX"
                     /locus_tag="ACHJ12_001204"
     CDS             complement(21239..22420)
                     /gene="cynX"
                     /locus_tag="ACHJ12_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4989026.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22426..22896)
                     /gene="cynS"
                     /locus_tag="ACHJ12_001205"
     CDS             complement(22426..22896)
                     /gene="cynS"
                     /locus_tag="ACHJ12_001205"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4989027.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22927..23586)
                     /gene="cynT"
                     /locus_tag="ACHJ12_001206"
     CDS             complement(22927..23586)
                     /gene="cynT"
                     /locus_tag="ACHJ12_001206"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4989028.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23696..24595
                     /gene="cynR"
                     /locus_tag="ACHJ12_001207"
     CDS             23696..24595
                     /gene="cynR"
                     /locus_tag="ACHJ12_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4989029.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24635..25918)
                     /gene="codA"
                     /locus_tag="ACHJ12_001208"
     CDS             complement(24635..25918)
                     /gene="codA"
                     /locus_tag="ACHJ12_001208"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4989030.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25908..27167)
                     /gene="codB"
                     /locus_tag="ACHJ12_001209"
     CDS             complement(25908..27167)
                     /gene="codB"
                     /locus_tag="ACHJ12_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4989031.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27506..29392)
                     /gene="prpE"
                     /locus_tag="ACHJ12_001210"
     CDS             complement(27506..29392)
                     /gene="prpE"
                     /locus_tag="ACHJ12_001210"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4989032.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29432..30883)
                     /gene="prpD"
                     /locus_tag="ACHJ12_001211"
     CDS             complement(29432..30883)
                     /gene="prpD"
                     /locus_tag="ACHJ12_001211"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4989033.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30917..32086)
                     /gene="prpC"
                     /locus_tag="ACHJ12_001212"
     CDS             complement(30917..32086)
                     /gene="prpC"
                     /locus_tag="ACHJ12_001212"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4989034.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32131..33021)
                     /gene="prpB"
                     /locus_tag="ACHJ12_001213"
     CDS             complement(32131..33021)
                     /gene="prpB"
                     /locus_tag="ACHJ12_001213"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4989035.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33259..34845
                     /gene="prpR"
                     /locus_tag="ACHJ12_001214"
     CDS             33259..34845
                     /gene="prpR"
                     /locus_tag="ACHJ12_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4989036.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEENMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(34946..35221)
                     /gene="yahO"
                     /locus_tag="ACHJ12_001215"
     CDS             complement(34946..35221)
                     /gene="yahO"
                     /locus_tag="ACHJ12_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4989037.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35368..36039
                     /gene="yahN"
                     /locus_tag="ACHJ12_001216"
     CDS             35368..36039
                     /gene="yahN"
                     /locus_tag="ACHJ12_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4989038.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36056..36301)
                     /gene="yahM"
                     /locus_tag="ACHJ12_001217"
     CDS             complement(36056..36301)
                     /gene="yahM"
                     /locus_tag="ACHJ12_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO4989039.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(36714..37529)
                     /gene="yahL"
                     /locus_tag="ACHJ12_001218"
     CDS             complement(36714..37529)
                     /gene="yahL"
                     /locus_tag="ACHJ12_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4989040.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37772..38821)
                     /gene="yahK"
                     /locus_tag="ACHJ12_001219"
     CDS             complement(37772..38821)
                     /gene="yahK"
                     /locus_tag="ACHJ12_001219"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4989041.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38908..39864)
                     /locus_tag="ACHJ12_001220"
     CDS             complement(38908..39864)
                     /locus_tag="ACHJ12_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4989042.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(39861..40832)
                     /locus_tag="ACHJ12_001221"
     CDS             complement(39861..40832)
                     /locus_tag="ACHJ12_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4989043.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40825..42309)
                     /locus_tag="ACHJ12_001222"
     CDS             complement(40825..42309)
                     /locus_tag="ACHJ12_001222"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4989044.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGWKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVEGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42358..43341)
                     /locus_tag="ACHJ12_001223"
     CDS             complement(42358..43341)
                     /locus_tag="ACHJ12_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4989045.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43595..44005)
                     /locus_tag="ACHJ12_001224"
     CDS             complement(43595..44005)
                     /locus_tag="ACHJ12_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4989046.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44304..45686)
                     /gene="yahJ"
                     /locus_tag="ACHJ12_001225"
     CDS             complement(44304..45686)
                     /gene="yahJ"
                     /locus_tag="ACHJ12_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4989047.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45696..46646)
                     /gene="yahI"
                     /locus_tag="ACHJ12_001226"
     CDS             complement(45696..46646)
                     /gene="yahI"
                     /locus_tag="ACHJ12_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4989048.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46789..48207)
                     /gene="yahG"
                     /locus_tag="ACHJ12_001227"
     CDS             complement(46789..48207)
                     /gene="yahG"
                     /locus_tag="ACHJ12_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4989049.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48207..49754)
                     /gene="fdrA"
                     /locus_tag="ACHJ12_001228"
     CDS             complement(48207..49754)
                     /gene="fdrA"
                     /locus_tag="ACHJ12_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4989050.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49744..50607)
                     /gene="yahE"
                     /locus_tag="ACHJ12_001229"
     CDS             complement(49744..50607)
                     /gene="yahE"
                     /locus_tag="ACHJ12_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4989051.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50647..51252)
                     /gene="yahD"
                     /locus_tag="ACHJ12_001230"
     CDS             complement(50647..51252)
                     /gene="yahD"
                     /locus_tag="ACHJ12_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4989052.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            51510..52007
                     /gene="yahC"
                     /locus_tag="ACHJ12_001231"
     CDS             51510..52007
                     /gene="yahC"
                     /locus_tag="ACHJ12_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4989053.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52099..53031
                     /gene="yahB"
                     /locus_tag="ACHJ12_001232"
     CDS             52099..53031
                     /gene="yahB"
                     /locus_tag="ACHJ12_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4989054.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53073..54161)
                     /gene="pdeL"
                     /locus_tag="ACHJ12_001233"
     CDS             complement(53073..54161)
                     /gene="pdeL"
                     /locus_tag="ACHJ12_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4989055.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54303..58286)
                     /gene="ehaA"
                     /locus_tag="ACHJ12_001234"
     CDS             complement(54303..58286)
                     /gene="ehaA"
                     /locus_tag="ACHJ12_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4989056.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        13914 a        15882 c        14591 g        14127 t
ORIGIN      
        1 aaaataacta tacataccaa acatacaaac acttaacata tgaattgcat aaaaacatta
       61 cccagtcgaa cctccgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat
      121 ttataaatta acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt
      181 agtataataa ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt
      241 tcttcggcaa ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa
      301 ttaggctgtg gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagcagtg
      361 gtgatgacta ccggcaactc tggtacagat ggaataatcg atcgccatga agaatcgcgc
      421 aacgccctaa agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc
      481 gctcatttac agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt
      541 ccttctgatg ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat
      601 catctcgctg tttatcaagc ttcaatggtt gcctgccgca ctattccaca aattctcggc
      661 tacgaaaccc caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa
      721 gaatatttca cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgtgat
      781 tatatgcgtc atgatcgcct gcgtgcagtt gcacaatttc gcgggcaaca ggtcaatagc
      841 gatctgggtg aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc
      901 tggatattta tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt
      961 aaacccagtc aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg
     1021 tgtctggcgc agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc
     1081 gttaacgacg gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg
     1141 ggcgaccgct ttgttgccgt cacgcaaaaa aatactggta aaggtggtgc gctgatgaat
     1201 ggcctcaatt atgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg
     1261 cccgatcaag acggaatggg ctatatgctg gcagaaattg agcgcggtgc tgatgccgta
     1321 ggcggcattc cctctactgc gttgaaaggc gcggggctgt taccgcacat ccgcgcgacc
     1381 gtaaagttgc cgatgattgt tatgaagcgc acgctacagc agctcctggg cggcgcaccg
     1441 tttattatca gcggtgcctg cgggatgttc cgtactgatg tattgcgtaa gttcggtttc
     1501 tcggatcgta ctaaagtcga agaccttgat ctcacctgga cattagtggc aaacggctac
     1561 cgtattcggc aggcgaatcg ctgcatcgta tacccacagg aatgcaacag tccgcgtgag
     1621 gagtggcgtc gctggcggcg ttggattgtg ggatacgcgg tctgtatgcg cctgcataaa
     1681 agacttttat ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgatt
     1741 tatggcgttg ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat
     1801 ggtgtggtgt tggcaatgtt tccgcttatc tggatcggcg tagtttgtgt tattggtgct
     1861 tttagcgcct ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg
     1921 tatgtattat tagcttatgc catctggatt atttatggac ttattgcctt ttttactgga
     1981 cgcgaacctc agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct
     2041 tattcccaac cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa
     2101 aaatattcag attgcagacc aggtaattat tgatgaaact gcaggtgaag ttgttatcgg
     2161 cgcgaatacc cgtatttgtc atggtgccgt tattcagggt ccggtagtga ttggcgcaaa
     2221 ctgcctgata ggtaattatg cgtttattcg tcctggcaca ataatcagca atggtgtaaa
     2281 aattggtttt gccaccgaaa ttaaaaatgc ggttattgaa gcggaagcaa cgattggtcc
     2341 gcaatgtttt attgccgact cggtagttgc aaaccaggca tatttgggcg cacaagtacg
     2401 taccagtaat catcgtctgg atgaacagcc tgtgtctgtt cgaactccag agggaattat
     2461 cgctaccgga tgcgataaat taggttgtta tatcgggcag cgttcacgcc ttggcgtaca
     2521 agttataatt ttgcctgggc gaattatttc cccgaacacg caacttggcc cgcgcgtgat
     2581 tgtagaacgt aatttacctg ccggaactta ttcactccga caagaactta tccgtacagg
     2641 agattaaaat gataaaacgg acgttattag cggcggccat ttttagcgca ttgcccgctt
     2701 atgccgggtt aacttccatt accgcgggct acgattttac cgattattct ggcgatcatg
     2761 gcaaccgtaa tttagcgtat gctgaactgg tggcgaaagt tgaaaacgca acgctgcttt
     2821 ttaatctttc ccaggggcgt cgtgattatg aaactgaaaa tttcaatgcc actcgcggtc
     2881 agggtgcggt ctggtataaa tggaataact ggctgacaac ccgaacgggt attgcctttg
     2941 cggataatac gccggtcttt gcccgccagg attttcgtca ggatattaac ctggccctgt
     3001 tgccaaaaac gcttttcacg accggttatc gctacactaa atattacgat gatgtcgaag
     3061 tcgatgcctg gcaaggcggc gtatcactct atactggccc ggtgatcacc agctaccgct
     3121 atacccatta tgactccagc gatgcaggtg gtagttatag caatatgatt tccgtgcgtc
     3181 tgaatgaccc gcgcggcact ggttatacgc aactatggct aagccgcgga acaggcgctt
     3241 acacctatga ctggacgcca gaaacacgct acggcagcat gaagagcgtc agtttgcaac
     3301 gtattcaacc gctgactgag caacttaatc tcgggctgac ggcaggtaaa gtgtggtacg
     3361 acaccccaac cgatgattat aacggtctgc aacttgcagc ccatctgacc tggaaattct
     3421 gattccttct gccgcccgct atccggggcg gccttccctg ccgattagcc ccccctttcc
     3481 tctttgtttt ccgaccacat tcaccggata aattttattc tccagtgtta tatactatag
     3541 gggggtatgc attgacatat agcatacccc cctatagtat attgcgtgca gatgatgagg
     3601 tgcgaaatgc ccagtactcc ggaagagaag aaaaaggtcc ttactcgagt tcgtcgtatt
     3661 cgagggcaga ttgatgctct ggaacggtcg ctggagggtg atgccgaatg ccgtgccata
     3721 ctccaacaga tcgctgccgt tcggggcgcg gctaacgggc tgatggcaga agtgcttgaa
     3781 agccatatcc gggaaacgtt tgaccgaaat gactgctaca gccgcgaagt cagccaatcc
     3841 gttgacgaca ctattgaact ggttcgagcc tatcttaaat agctgaatct attaccatat
     3901 tgaggaagag cgagagatga aatcacgtgc tgccgttgca tttgctcccg gtaaaccgct
     3961 ggaaatcgtt gaaattgacg ttgcaccacc gaaaaaaggt gaagtgctga ttaaagtcac
     4021 ccataccggc gtttgccata ccgacgcatt taccctctcc ggggatgacc cggaaggtgt
     4081 attcccggtg gttctcggtc acgaaggggc cggcgttgtg gttgaagtcg gtgaaggcgt
     4141 aaccagcgtc aaacctggcg accatgtgat cccgctttac accgcggagt gcggcgagtg
     4201 tgagttctgt cgttctggca aaactaacct ctgtgttgcg gttcgcgaaa cccagggtaa
     4261 aggcttgatg ccagacggca ccacccgttt ttcttacaac gggcagccgc tttatcacta
     4321 catggggtgc tctacattca gtgaatacac cgtggtcgcg gaagtgtctc tggccaaaat
     4381 taatccagaa gcaaaccatg aacacgtctg tctgctgggc tgtggcgtga ccaccggtat
     4441 tggcgcggtg cacaacacag ctaaagtcca gccaggtgat tctgttgccg tgtttggtct
     4501 tggcgcgatt ggtctggcag tggttcaggg cgcacgtcag gcgaaagcgg gacggattat
     4561 cgctatcgat accaacccga agaaattcga tctggcgcgt cgcttcggtg ctaccgactg
     4621 cattaacccg aatgactacg acaaaccgat taaagatgtc ctgctggata tcaacaaatg
     4681 gggtatcgac catacctttg aatgcatcgg taacgtcaac gtgatgcgtg cggcgctgga
     4741 aagtgcgcac cgcggctggg gtcagtcggt gatcatcggg gtggcaggtt ctggtcagga
     4801 aatctccacc cgtccattcc agttggtcac tggtcgcgta tggaaaggtt ccgcgtttgg
     4861 cggcgtgaaa ggtcgttccc agttaccggg tatggttgaa gatgcgatga aaggtgatat
     4921 cgatctggaa ccgtttgtca cgcataccat gagccttgat gaaattaatg acgccttcga
     4981 cctgatgcat gaaggcaaat ccattcgaac cgtaattcgt tactgatttc ccgcaggtat
     5041 accccgtcca cttcagacgg ggttcttaat actctccccg ggcagccgtc cgggggatta
     5101 accgtgagat aatgactgat ggaactcatt gaaaaacatg ccagctttgg cggctggcaa
     5161 aatgtgtatc ggcattattc ccaatcactg aaatgtgaaa tgaatgtcgg cgtctatctc
     5221 ccaccaaaag ccgcgaatga aaaattgccg gtgctgtact ggctttcggg cctgacctgc
     5281 aacgagcaga atttcattac taaatcgggg atgcagcgtt acgcggctga gcacaacatt
     5341 attgttgttg cgccggacac cagtccgcga ggcagtcatg tcgcagatgc cgaccgttac
     5401 gatctcgggc aaggtgccgg gttttacctg aacgcaacgc aagcgccgtg gaatgaacat
     5461 tacaaaatgt atgactatat ccgcaacgag ctgccggatt tagtgatgca acattttccg
     5521 gcaacgacca gaaagtctat ctctggtcat tctatgggcg ggctgggcgc gctggtgctg
     5581 gcgttacgta acccagatga atatgtcagc gtctcggcgt tttcgcccat tgtctcccca
     5641 tcgcaagtac cgtggggaca gcaagccttt gccgcatatc ttggtgaaaa taaagatgcc
     5701 tggttggatt acgatccggt gagtcttatt tcacaaggtc aacgcgttgc ggaaatcatg
     5761 gttgatcagg ggttgagtga tgatttttac gcggaacagc tgcggactcc aaatcttgaa
     5821 aagatctgcc aggagatgaa tatcaagacg ttaatccgtt atcacgaggg ttacgaccac
     5881 agctattatt ttgtctccag ttttattggc gagcatattg cctaccacgc caataaactg
     5941 aatatgcgtt gataatagtg cacgactgcc ggatgcggcg tgaacgcctt atccgcctac
     6001 atttcgcccg caggccgtag gcatgataag acgcgcaaag cgtcgcatca ggcatggcgc
     6061 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgccca taaaccgtag
     6121 gcctgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac
     6181 cacatcaaat catcaggcac tttgaagtcg gcatacgggt cgtcttcatc ctgctcttcg
     6241 gcactgagcg cgctgtgcaa cacaatactg ctggcatcac gctgagcaat tttatcggca
     6301 acgctggcgg gaataatggc gtattcacct tcgctattgt tatcgaccaa caagcgggca
     6361 atcgccagac gaccattaat caactgcgct tgtgtgagct tatcgacaaa aatcttctta
     6421 atcagattgc cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc
     6481 atttcaatga gctgcttcac ctgagcttta tattctttcg ccaacgccgc ttgtttttgc
     6541 tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgccgcc
     6601 cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt
     6661 ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt
     6721 gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt gggtgtattg
     6781 ggggcaagtc cctttcgccc ctgcttaata aggcgcgtca ggcatcggca cacggctgca
     6841 ttcttgatct ccgactcatc aaaataaaca ccgccaaccc agcaacaaga atacccggtg
     6901 cagaagcggc cattacgccg accgtgccag tgcctaatgc cagcattttc ccggccagta
     6961 acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc
     7021 ggatctgcga actgtaaaac aacggtgcca gggcatacaa aacgctttgt ccacctgtcg
     7081 caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac accgttccaa
     7141 gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca
     7201 gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagggcaa
     7261 acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt agcggtagcc
     7321 agttgatcaa catataaacc accagcagag tgaagaaata acacaaccac agcagcagcg
     7381 tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc
     7441 cagcgaaaac cgctgactcc ggcagccagc gcattaatag cggcaccaga atcaacggca
     7501 ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg
     7561 tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac
     7621 gaaaacgtgg acccgcggct tcagacgtca gggcgataag attcggcaac gccgccccca
     7681 gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg
     7741 ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatacgc ttacgaccat
     7801 aacggtctgc cagcatcccg ccaaccaacg cgccgggtag caaaccgagt attccggcgc
     7861 taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg ccacccgccg
     7921 caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga
     7981 tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct
     8041 cattgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc agaactattt
     8101 aatgactgcc tacgggcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc
     8161 gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc atcattattt gttgttgcgc
     8221 agatccagcg ccacgtcaac gatcatatcc tcctggccgc caaccatccg gcgtttgccc
     8281 agctcaacga gaatatccac cgcacttaag ccataacgcg ccgccgccgt ttcacagtga
     8341 cgcaggaagc tcgagtaaac accagcgtat cccagcgcca gcgtttcgcg atcgactcgt
     8401 accggtcgat cctgcaacgg acgcaccagg tcgtcggcgg catccattaa cgcatagaga
     8461 tcggtcccat gctgccagcc cagtttatcc gcggcggcaa taaacacttc cagcggtgcg
     8521 ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga ttcggtcgca gccctcttcc
     8581 accgccgcga tagagttcgc cacgccaaga ctcaggttat ggtgagcgtg catgccagtt
     8641 tgcgtttctg gtttcagctc tgctttcagg gcgcggaaac ggtcacggat atcgctcatg
     8701 ttcatcgcac cgccagaatc caccacataa atacaggtcg caccgtagcc ttccatcagc
     8761 tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc tcatcatcag aaaaccaacg
     8821 gtgtccattc cgagctcgcg ggcatactga atatgctggg cggaaacatc agcttcggta
     8881 cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc aggcattttt cagatcgtga
     8941 atagtgccga ttcctggcag caacaacgtc gcgattttgg cgtgcttcac cacatccgcc
     9001 gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac catagttaaa gctggaaccc
     9061 tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc gggcatcgtc cagtgctttg
     9121 gcaatctggc gaacgttttc cagcgaatac tgatgacgaa tggcgtgcat accgtcacgc
     9181 aacgtgacgt ccgagatata aagtttttta ccgttcatgc cgcttctcct gccttgcgcg
     9241 ccagtgactg ggccattttt tccgctgtcg ccagcgcact ggaagtcata atgtcgaggt
     9301 tgcccgcata ggcaggcaga taatgcgctg cgccttcgac ttccagccag accgctgttt
     9361 tcagtccgga gaattgcccc acgcccggta aattgaccgg tttatcctgc gggataactt
     9421 caaactgcac gcgctgtttc aggcgataac ccggtacgta agcctgcacc gcctcagcca
     9481 tttcattgat tgaggcttcg atatcatctt gtgaagcttc gtcgctcaat acatacaccg
     9541 tgtcacgcat catcaacggt ggctctgctg ggttaagcac aataatcgcc ttcccttttg
     9601 ccgcgccgcc caccacttca atggctcggg aagtggtttc cgtaaattca tcgatattgg
     9661 cacgcgtgcc aggtccggca gatttactgg cgatagaagc gataatttcg gcgtaatgaa
     9721 cacgcgccac gcgtgaaact gccgccacca ttggaatggt ggcctggccg ccgcaggtga
     9781 ccatgttgac gttcagttga tcgacgttcg cctcgaggtt aaccaccggc acgcagtaag
     9841 ggccgatggc agcaggcgtc aggtcaatta agcgaatatc cggtttcgct tcgcgtaaag
     9901 cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc aaatacaatg tcgatatcag
     9961 caaattcagg catgttcatc agcccgatca ccccttcatg ggtggtggcg acgcccatac
    10021 gtctggcacg cgccagaccg tcggactgag gatcaatgcc gaccatcacc gccatctcca
    10081 gatgctgacc gtgacgcaaa attttaatca tcagatcggt gccaatgttg ccagaaccga
    10141 taatggcgac tttacgctta ctcatgacag acttcctttt ggggctgcgc ttgaaaatgt
    10201 cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa cgatcgcccg cattcactgc
    10261 caccatcgga cctaatgccc cggtaagaat gatatctccg gcgcgcagcg gttcacccag
    10321 gctggccatt ttgcgtgcca gccagacggc ggcattaagc ggatgtccca ggcattcgct
    10381 gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc ttcatggcgc agtttttcag
    10441 gtctaacccc gccggacgtt gcgccggacc gccgatgaca tacaccccgc aggaagcgtt
    10501 atctgccacg gtatcgacaa actgaatcga ccagtcgcga atgcggctac ccaccacctc
    10561 cagcgccgga agtacccatt caatggcgtt atacaattcg tcgaaggtga tatcggttgc
    10621 gggcaaatcg cggttcaaca ccagtgcgat ctccgcttca atgcgggctt gcagaacacg
    10681 ggaaaaagga atgatttcgt tatcgccata acacatgtcg gcaaataatg tcccgaaatc
    10741 cggttgatca acgcccagtt gttgctgcac tttcggatgc gtcaggccca ctttacgccc
    10801 taccacgcga cgcccctgcg caacgtcata ttgcacattt atgtgctgaa tggcgtaggc
    10861 cgcttcagcg ttatcgatac caatcagatc gcgcagcggc gcaatcgctt cgccctgctc
    10921 tgcagcgcgg cgtaaatccg ccgccagttg ctcaagagta tgcttcgtca tgaccattcc
    10981 ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa gcgtcggcat gttcccactg
    11041 cgcccagtga ccacagtcgc ggaagatatg cagttcagaa ccggcaatgc cggacagcag
    11101 acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc ccccagacaa tcagggtttg
    11161 cgctttgatt tccgccagac gtgggccaaa atccgggaac tgtttcggat tagcttccag
    11221 gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc atattattca ggcgcgcttc
    11281 aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag atatccatca tcagcttcag
    11341 gttttcgata gtcggctgac gataaagctg attcagtcgc ttaatacctt cggttggcat
    11401 cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc agcaccagtt tgccgacgcg
    11461 ctccggccag ttaagggtga acgccacaga actatggccg cccatcgagt tgcccagcag
    11521 gtggattttg gcgatatcca gttgatccac cacgcttttc aggattcgtg cattaagatc
    11581 cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc caacccggac aatccagcag
    11641 gatcacccga tagcccgcct ctaccagcgg atcgatattg cggctgaagt tcgcccagcc
    11701 agtagcaccc gggccggaac cgtgcagcag gacaacggtt tcgtcgcctt gtccgcagtc
    11761 attaaaatgg atgcgcagcg ttttacccgc ttcttctaca ttcagaaaac ggctggtggc
    11821 ggcttcggtt tgtggctgat aactcatcat cttctcctgc atattcagtt ctctgttctg
    11881 gcgcttaacg agccaaatcc ggcaatccac tccgggattg ggcggtaata acgcccttcg
    11941 ctacgccagt tgccaaatgt agaaatagcg gcaaaagcgg cgacccaggt tttgatttca
    12001 tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac tgacggcatc cagttcctgt
    12061 atgcgtccct gctccagcaa agtcatgaac tggttatccc aaatcgggtt gagcggatgc
    12121 agagttctct gatcctcaac aaacttctca gcggcgctaa tcacccgttg ctgacgcaat
    12181 tcgcgctcac tggcgggtaa atctttcccg ctccccaaca gacggtcgcg catatgggca
    12241 tcggctttcg ccagttcggg caccggcggc tgatgggaaa gcccaccgga acccaggaac
    12301 agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg cttcacccaa catgcgggta
    12361 cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga acacaggcag aactggcacc
    12421 ttatccagcc cgccgagcag gaactccagt ggctgggcga acccgtggtc cacctgcata
    12481 cagtaagaaa cggcaagatc gatcccgctc ttcatgacgg catgcgcaca ggcctccgcc
    12541 agctccacag gcacgggcag ctctcctgcc gcactgccga aatcaccaat tgccgtcgct
    12601 ccaacgccta aacagaacgg tggcatcacg tcatagaaaa agccgttgta gtgatcaggc
    12661 gcaaacagca ccaccagttc aggggagaaa gccgcaatac gctcgcgggc gctggcaatc
    12721 acgccattga cctcatcgag cacctcttgc gccgggtcga cataccccac cagcggcgag
    12781 tgggaaagac agtgaagata agcgtgcata tcaggctacc ttttcgacag aaacgtcggc
    12841 atcagggcgg gtcagcgtca tcaccgacgc cagtttattc agggtcttgc ccagggtttg
    12901 cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc accaccagcg aagcattgtg
    12961 ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc acgcgtagca cgccgtcgtg
    13021 gttatcctgt gcggtatgaa tttgcacttc cggcaccacc tgaatgaagc gtgtgcccaa
    13081 cgcgcgccac tgctggattt gctcgtcgct catcccccac agtggattgc atccccagcc
    13141 aattaccgcg aagttcgcgc caatcgcgtt atcgagcagc gtcacgtcac cgttttccag
    13201 cgtgactttc ggctgaataa acatcttgcc gaccggagag tgcttcgctt cgccctcacg
    13261 caccagcgca ccgccgtaat attgcggcat cggcttgaag cgcatttcga ggaagtagcg
    13321 ttttactggc ggcagataat tcagtagcca ggaaacgccg tcacgtaacg taccctgcca
    13381 gcgtttcggc ggagccagca cgttgcccgc cgtcacggac aggtcaatca tcgctttggc
    13441 gtgatcgcga cgttcttgtt gataggtatc gagcagcgca tcgcgggctt tcccctggat
    13501 aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc ataccactgt tatagccctg
    13561 cccctgccat accggcatga tgtgcgcggc atcgcccgcc agcagtacgc gatcaatacg
    13621 gaaacgttgc gccagtcgcg cgttgtgggt gtagacacgc tggcgaatca attcaacatt
    13681 gtccggatta ggcagcactt tgcttaacag cttgcgcata ttttgcggct cacgcagctg
    13741 ttcttcggtt tctcccggca tcaccataaa ttcaaagcga cgtaccgcat gaggcagcgc
    13801 ggcagaaaca tacgggcgca ccggatcgca acataaatag atatgcggcg tacttaacgg
    13861 atcgttggcg atatccacca caatccactg atttggcgca gttttacctt caaacggcac
    13921 attcagggtg cgacgaacaa aacttgcccc accatcacag gccaccagcc actgggcttt
    13981 gactatttcc cgctgccctt ctgccgtttt caggtgcaag gtcacttcgt cattttgctg
    14041 gctgaaggcc tccagctcgc gggcaaacaa gcagcgcaca ttcggaaaac gcgacaaccc
    14101 ttccagcatc accgcatcga cctgtggctg aataaaggcg ttacggcgcg gccagccaaa
    14161 ttcatcggtc attggctgaa tatcagcaaa acagcggcct tttggggtga gaaaacgcat
    14221 cgcgtgccac ggtgtagtgt gcggcagaac attctcgacc aggccgaccg actgcatggt
    14281 gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag tcgatcaact tatcgagttt
    14341 ctccaccacc agcacgtcaa tgcccatctg accgagatag ttcgccatca tcagcccaac
    14401 cggaccggca ccagcgatcg ccacctgaac gctatggtta acagcaggct ggatgtcagg
    14461 gtgttgtatt gccatttcag tacctcacga ctcggacaaa atgccgttgc gcgcacagta
    14521 cagcgcaact tattttgtta aaaacatgta aatgattttt tattgtgcgc tcagtatagg
    14581 aagggtattt tcggctacaa tcaaaacatg cccgaatgtg caccaggtgc acaacgttgt
    14641 tttaactata gaaatgtcaa ttaatatgca gaacaatgag cagacggaat acaaaaccgt
    14701 gcgcggctta acccgcggtc taatgttatt aaatatgtta aataaacttg atggcggtgc
    14761 cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc accactgtgc ggcgactgct
    14821 ggagacgctg caggaagagg gatatgtccg ccgtagcccc tccgatgata gttttcgact
    14881 gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac gaacagtgga tttctgcact
    14941 ggcggccccg ctgctgggcg atctgttgcg cgaagtggta tggccaacag atgtgtccac
    15001 cctggatgtt gatgcaatgg tggtacgcga aaccacccac cgtttcagcc gcttatcctt
    15061 tcaccgggca atggtcgggc gacgtttacc gctcctgaaa accgcctcgg gcctgacctg
    15121 gctggccttt tgcccggaac aagaacgcaa ggaattaatc gaaatgttag ccgcccgccc
    15181 cggtgatgac tatcaactgg cacgagaacc gttaaagctg caagccattc tggcgcgcgc
    15241 gcgcaaagag ggttacggac agaactaccg cggctgggat caggaggaga agattgcctc
    15301 tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat
    15361 ggcgagcgca atgaccattg aacaggcagc ggaaaagcat cttccggcgc tacaacgggt
    15421 agcaaaacag atcgaagaag gggttgaatc gcaggctatt ctggtggccg gacggcgaag
    15481 cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac ctttcgtggt atggcatgat
    15541 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg
    15601 tcgcagagta tgccggtgtc tcttatcaga ccgtttctcg cgtggtgaac caggccagcc
    15661 acgtttctgc gaaaacgcga gaaaaagtgg aagcggcgat ggcggagctg aattacattc
    15721 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
    15781 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
    15841 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
    15901 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
    15961 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
    16021 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
    16081 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
    16141 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
    16201 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
    16261 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
    16321 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
    16381 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
    16441 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
    16501 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
    16561 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    16621 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    16681 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    16741 gaaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc
    16801 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttacac aacttaatcg
    16861 ccttgcggca catccccctt tcgccagctg gcgtaatagc gaagaagccc gcaccgatcg
    16921 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
    16981 agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
    17041 cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc
    17101 cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
    17161 atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
    17221 taactcggcg tttcatctgt ggtgcaacgg tcgctgggtc ggttacggtc aggacagtcg
    17281 tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
    17341 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
    17401 cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca
    17461 tgttgccact cactttaatg atgatttcag ccgcgcggta ctggaggcag aagttcagat
    17521 gtacggcgag ctgcgcgatg agctgcgggt gacggtttct ttgtggcagg gtgaaacgca
    17581 ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
    17641 cgatcgcgtc acgctacgtc tgaacgtcga aaacccggcg ctgtggagcg ccgaaatccc
    17701 gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
    17761 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
    17821 caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt
    17881 catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
    17941 cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
    18001 cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct
    18061 gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
    18121 cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
    18181 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta
    18241 tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
    18301 ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
    18361 acctggagaa ctgcgcccac tgatcctttg cgaatatgcc cacgcaatgg gtaacagtct
    18421 tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
    18481 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtt
    18541 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgca tgaacggtct
    18601 ggtctttgcc gaccgcacgc cgcatccggc gctgacggaa gcaaaatacc agcagcagtt
    18661 tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
    18721 tagcgataac gagctcctgc actggatggt ggcgctggat ggcaagccgc tggcaagcgg
    18781 tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctggactacc
    18841 gcagccggag agcgccggac aactctggct tacggtacac gtagtgcaac cgaacgcgac
    18901 cgcatggtca gaagccggac acatcagcgc ctggcagcag tggcgtctgg cggaaaacct
    18961 cagcgtgaca ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
    19021 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc
    19081 acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg
    19141 cgcgccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc cgaacgcctg
    19201 ggtcgaacgc tggaaggtgg cgggccatta ccaggccgaa gcggcgttgt tgcagtgctc
    19261 ggcagataca cttgccgacg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg
    19321 gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
    19381 taccgttgat gttgaagtag cgagcgatac accgcatccg gctcggattg gcctgacctg
    19441 ccagctggca caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
    19501 tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
    19561 taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
    19621 tggcccacac cagtggcgcg gcgacttcca gttcaatatc agtcgctaca gccaacaaca
    19681 actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
    19741 cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
    19801 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
    19861 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa
    19921 aaacacaaac ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggagc
    19981 ctacttcccg tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac
    20041 gggtattatt tttgccgcta tttctctttt ctcgctatta ttccaaccgc tgtttggtct
    20101 gctttctgac aaactcgggc tgcgcaaata cctgctgtgg attattaccg gcatgttagt
    20161 gatgtttgcg ccgttcttta tttttatctt cgggccactg ttacaataca acattttagt
    20221 aggatcgatt gttggtggta tttatctagg cttttgtttt aacgccggtg cgccagcagt
    20281 agaggcattt attgagaaag tcagccgtcg cagtaatttc gaatttggtc gcgcgcggat
    20341 gtttggctgt gttggctggg cgctgtgtgc ctcgattgtc ggcatcatgt tcaccatcaa
    20401 taatcagttt gttttctggc tgggttctgg ctgtgcactc atcctcgccg ttttactctt
    20461 tttcgccaaa acggatgcgc cctcttccgc cacggttgcc aatgcggtag gtgccaacca
    20521 ttcggcattt agccttaagc tggcgctgga actgttcaga cagccaaaac tgtggttttt
    20581 gtcactgtat gttattggcg tttcctgcac ctacgatgtt tttgaccaac agtttgctaa
    20641 tttctttact tcgttctttg ctaccggtga acagggtacg cgggtatttg gctacgtaac
    20701 gacaatgggc gaattactta acgcctcgat tatgttcttt gcgccactga ttattaatcg
    20761 catcggtggg aaaaatgccc tgctgctggc tggcactatt atgtctatac gtattattgg
    20821 ctcatcgttc gccacctcag cgctggaagt ggttattctg aaaacgctgc atatgtttga
    20881 agtaccgttc ctgctggtgg gctgctttaa atatattacc agccagtttg aagtgcgttt
    20941 ttcagcgacg atttatctgg tctgtttctg cttctttaag caactggcga tgatttttat
    21001 gtctgtactg gcgggcaata tgtatgaaag catcggtttc cagggcgctt atctggtgct
    21061 gggtctggtg gcgctgggct tcaccttaat ttccgtgttc acgcttagcg gccccggccc
    21121 gctttccctg ctgcgtcgtc aggtgaatga agtcgcttaa gcaatcaatg tcggatgcgg
    21181 cactgggcac atccgacaat tatttatagc tccatcatct ggcgaaacaa gcgtcgcatc
    21241 atacctcttt gacccacagc tgcggaaaac gtgctggtgc aaaacgcagg gttatgatca
    21301 tcagcccaac gacgcacagc gcatgaaatg cccagtccat caggtaattg ccgctgatac
    21361 tacgcagcac gccagaaaac cacggggcaa gcccggcgat gataaaaccg attccctgca
    21421 taaacgccac cagcttgcca gcaatagccg gttgcacaga gtgatcgagc gccagcagca
    21481 aacagagcgg aaacgcgccg cccagaccta acccacacac catcgcccac aataccggca
    21541 attgcagcgg caaccagata aagccgcaga accccaccag ttgtaacacc agcgccagca
    21601 ttaacagttt gcgccgatcc tgatggcgag ccatagcagg catcagcaaa gctcctgcgg
    21661 cttgcccaag cgtcatcaat gccagtaagg aaccgctgta ctgcgcgctg gcaccaatct
    21721 caatatagaa agcgggtaac caggcaatca ggctggcgta accgccgtta atcagaccga
    21781 agtaaacacc cagcgtccac gcgcggggag tgaataccac gcgaaccgga gtggttgttg
    21841 tcttgtggga agaggcgacc tcgcgggcgc tttgccacca ccaggcaaag agcgcaacaa
    21901 cggcaggcag cgcccaccag gcgagtgttt gataccagga ttcgctatgt tgaactaacc
    21961 agggcgttat ggcggcacca agtccaccgc cgcccatcag agccgcagac cacagcccca
    22021 tcaccagtgg cgtgcgctgc tgaaaccgcc gtttaatcac cgaaggcatc accgcctgaa
    22081 tgatgccgat ccccacccca ccaagcagtg cgctgctaag cagcagcgca ctttgggggt
    22141 aaagctcacg catcaatgca ccgacggcaa tcagcaacag actgatggcg acactgcgac
    22201 gttcgctgac atgctgatga agccagcttc cggccagcgc cagcccgccc atggtgacca
    22261 ccggcagagc ggtcaacagg gcagccacgc taaagctcat tccgctcgcc tggcgcaatt
    22321 gcggtagcag tggcccgacg gaggtgagca gtggtcgcat attaagacca atcagcacca
    22381 gaaccagcag catcgtgaac gtcttttgat gattgcgcat ggctgtcaga acggcttggt
    22441 cggcagatat ttaccatcta aggtaatgac cgcacgttcg ccaccttccg ggtccgccac
    22501 tttcttaacg tcgagtttga agttaatcgc gctgataatg ccatcgccaa acttctcatg
    22561 aaccaacgct ttcagggttg tgccgtacac ctgcaacatt tcatagaaac gatacatcgt
    22621 tgggtcggtt ggaatacggt catcaatgca gccacgcagt ggaatcatct gcaacagtag
    22681 aatggcgtct tcgtcgagat ccagcttcgc cccgaccagg cgggcggcgt cggcaggaag
    22741 cgcttgctgc cccagcaaag ccgcggttac gaaggcttct gccagaccgg taccgtcggc
    22801 aatctcggca aatgagagat cttttttagc tttgctgagc aaaatggcat cggcaagatc
    22861 aagacgaata ttgcggttaa tttgtgactg aatcatggtg gatctcctga tggtttaaaa
    22921 ataaggttac gctgcggtcg gttggcgtag cggtatggca caaacgcgag gattagcggc
    22981 cagtggcaca aactggcggg ttgcgccgtc aaaagctgcg atgctgccgc tttcaatgtc
    23041 gtagacccag ccgtgcaggg cgatccgccc ctcttcgagc gccaggcgca ccgatggatg
    23101 agtttgcaaa ttagccaact gagcaatgac gttttcacgt accatcgccg cagcttttga
    23161 cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg gctgaatcgg cataacgcag
    23221 ccagtgggag acggcaggca tatggtccat gcactgacag ctggcaatgg cggtcatcgc
    23281 gccacagttg gaatgaccac aaatcacaat gtcagatacc cgaagcgcag cgacggcata
    23341 ctccaccgaa gcagaaacgc caccgggttc cggcccgtag gaagggacga tattgcccgc
    23401 gttgcgaata acgaacagat cgccaggctc acgttgcgtc accagctcag ggaccagacg
    23461 gctgtcggag caggagataa aaagtgtgcg cgggctttgc tgtgtcgcca gctgtttaaa
    23521 caaggcttcc cgcttcggaa atgcctcgcg ctggaattta aggaatccat caataatctc
    23581 tttcaccgtt aacctctgtc tgtctctgga atgagaggca gactactcgc cgattatcat
    23641 aaggtaaaag tctcatttat gatgagttct attggattta cttataggtt ggcgaatgct
    23701 ctctcgacat atcaactatt ttcttgccgt ggctgaacat ggcagcttta cccgtgccgc
    23761 cagtgcgttg cacgtctccc agcctgcgct ttcccagcag attcgccagt tagaggagag
    23821 tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt cgtctcactg atgcaggaga
    23881 agtctggcga cagtacgcca gccgggcgtt acaggaactg ggggcgggta aacgggcgat
    23941 tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc gccgtcactc ccacctttac
    24001 aagctacttt atcggccctt taatggcgga tttctatgcg cgctaccccg gcatcacgct
    24061 ccagctacag gaaatgtcgc aggagaaaat cgaagatctg ctttgtcgcg acgagctgga
    24121 cgttgggatt gccttcgcgc ctgtgcattc gccggagctg gaggcaattc ctttactgac
    24181 agaaagttta gcgttagtcg tggcgcaaca tcatccgctg gctgcctgtg aacaggtggc
    24241 gttgagtcgc ttgcatgatg aaaaactggt cctgctcagc gcggaatttg ccaccagaga
    24301 gcaaattgac cactactgcg agaaagcggg gctacatcca caggtggtca ttgaggcgaa
    24361 ctcaattagc gcggttctgg agctgattcg ccgcacttcc ctttccacat tgttaccagc
    24421 agcgattgcc acacaacatg acgggcttaa agcgatttct cttgccccgc cactactgga
    24481 gagaacggcg gttttgttgc ggcggaaaaa tagctggcag acagccgccg cgaaggcatt
    24541 tttgcacatg gcgttggagg aatgtgcgga tgttggcgag aatgaatcac ggtagccact
    24601 gacacaggtc agtggctctt caacacagag gaaattaccg tttgtaatcg atggcttctg
    24661 gctgctccag atatacggtg gtttgtgccg gttgtgtact ggcaatcacc ttgccgccac
    24721 gtaccgaata acgtaccgga acctgacggc gcagcgcatc aaatccattt tcagccggca
    24781 ggataatcag gtttgcgctg tttccggcgg caatgccgta atcctgcaaa ttcaatgttc
    24841 tggcactgtg gtgggtgatt aaattcaggc catcgttaat ctgcccgtag cccatcaact
    24901 ggcaaacatg cagccccata tgcagcactt gcagcatatt cgccgttccc agcggatacc
    24961 acggatcgaa gacatcatcg tgaccaaagc agacgttaat gccggactcc agcatctctt
    25021 taacgcgcgt gatgccgcga cgttttggat acgtatcgaa acgtccttgc agatgaatat
    25081 tgaccagcgg gttggcgaca aagttaatac cggacatttt cagcaagcgg aacaggcgtg
    25141 aggtatacgc cccgttatag gagtgcattg ccgtggtgtg gctggcggtg actcgcgcgc
    25201 ccatgccttc acggtgcgcc agggcagcaa cggtttcgac aaagcgcgac tgctcgtcat
    25261 cgatctcatc acagtgaacg tcgatgagac ggtcgtattt ttgcgccagg gcgaaggttt
    25321 tatgcagcga ctccacgccg tattcacggg taaattcaaa atgcggaatc gcccccacta
    25381 catctgcccc taagcgtaac gcctcttcca gcaacgcttc accgttggga tacgacaaaa
    25441 tcccttcctg agggaaggcg acaatttgca gatcaatcca cggcgcgact tcctgcttca
    25501 cttccagcat tgctttcagc gcagttagcg ttgcatccga aacatcgaca tgggtacgca
    25561 catgctgaat gccgttggca atctgccatt tcagcgtttg ccaggcgcgt tgtttcacat
    25621 cgtcatgggt taataacgct ttgcgctcgg cccagcgttc aatgccttca aacagcgtgc
    25681 cggactgatt ccagttcggt tgtccggcgg tttgcgtggt gtccaggtga atatgcggct
    25741 ccacaaacgg cggtataact aaaccttgtt cggcatccag gctgttttca gttatgggca
    25801 tcacgccgga ttgcgcatca atggcgctga tttttccgtc ctgcagatga atctgccaca
    25861 gcccctcttt gcctggtaac cgggcgttaa taattgtttg taaagcgtta ttcgacactg
    25921 ttaacctcca catgcgtcat tgctgctgtc gttttacgat tcaaaatcgg gttaaggatc
    25981 agatagctca gcgcgccacc taataccgcg ttgaccggaa caattcccgg taaccagtgg
    26041 cctgcggcaa tccccagcgc gaccgccaga atcgccaccc aattgacact catcatacgc
    26101 gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat agtcggcgat gatcacgccc
    26161 cctaccggag gaatagctgc cgaaaggaag gtcaaccagc cgacaaaatt gttatacagc
    26221 cataatgcgc agaccgtacc gataataccg ttgattaccg aaagggtttt gctcgacata
    26281 ccggtaatgt tggcgaaacc taaacccgac gcatagagtg cgttatcgtt ggtggtccag
    26341 atattcagcc ccagcaccac aatcgcaggc agcagcaggc cctgagcaat catcacatca
    26401 gagatatccg ccatgcccag tgccgcagcg cccgctgcac cgaaaataaa catcaacgag
    26461 ttgccgagga aaaaggccac catcgccacc agcaccgcca gtttggcatt gcgaccaaac
    26521 cggacaaagt cagcggtgag cgtacccgca ctgataaatg accccacaac cagcgccagc
    26581 gcgacattga aatctaacgg ttgtgcggga acgaccgctt ttaatgcgtc caggccgccc
    26641 atgccgttaa cagccagcca cacggaataa ccgcccaggc aggcgatagc cggaaccgca
    26701 atcaccgaaa gaaccgtcag cgccgaaatg ccaaaaaaga cggtgacggt catcagtaaa
    26761 ccggaaacgg caatcagcaa attaatatcc agcccggttg ccttacccac cggaatggca
    26821 aacatcgcca caccgacgcc aaaccagcca acctgagttc cgcccagtag cagtgaaggc
    26881 agccatgagc ctttaacacc aaacgagaag cgagcaagaa gatgagtggt caggccggtt
    26941 tttgcgccaa tgtaaccgag aaatgaagtg taaataccga ggagaagatt accgatgaga
    27001 actgcgagga agaaatcatg atagctaaga ccggttccga gagtgccgcc ggtccacata
    27061 ctggcggaaa agaaggttaa tcccagcatg acgaacgtca atgccaatac ccctttccgc
    27121 gccgactgcg ggactggccc ctggctaaag ttgttatctt gcgacacgaa attccccctt
    27181 tttgctgttt gaaacccaaa aaatccgccg cattctattc atctgaagat aaaaagcaat
    27241 cgttttcgtg gggaaatata ttttttatat gaatgagtgc aaaaaggtgg gggaaaaatt
    27301 atcggtgtaa aaacagtggg tagtgggtag atactgataa gacgcgtcag cgtcgcatca
    27361 ggcatcggcg catggtgtcg gatgcgacgc gagcgcctta tccgacctac ggttcaggcc
    27421 tgtaggcata ataagacgcg gtaagcgtcg catcaggcat cggcgcatgg tgtcggatgc
    27481 gccacgcgac gcatccgaca ataccttact cttccatcgc ctggcggatc tgatccaatg
    27541 acgcaggatc atcaatggtc gtcagatctc caggatcgcg tccttcgcaa atcgcctgga
    27601 tcgtgcggcg cagcattttt ccggatcgcg tttttggcaa ttgcgagaca aaccagacgt
    27661 gcgccgggcg gccaaagttg ccaatctggc tgtccaccag cgccataatc gccttctctt
    27721 gcgaatgcgc cacatcacga tcttccagac tgtcgctctc tttcggaatg acaaacgcta
    27781 ccgccacctg ccctttcagc gcatctttca ccccaaccac cgccacttcg gcaacgcccg
    27841 gatgactgga aatactctct tcaatctcgc gcgtgcccag ccgatgcccg gcaacgttaa
    27901 ttacatcgtc agtgcgcccg agaataaagt gataaccgtc agcgtcacgg atgccccagt
    27961 caaaggtggc gtacaccggg cgggaaaaca gcgaccagta agtcttcaca aagcggtcgt
    28021 cgtcgcccca gatggtctga atacagcccg gcggcagcgg cccttccacc accagcatcc
    28081 ctttctcgtt gacgccacac ggttcgccgg tgacttcatt aagcaactgc acgttatagc
    28141 catacatcgg cacgccgggg cttcccagac gcgtcggcct gtcgtccaga ccgcgagcaa
    28201 tcgccataat cggccagccg gattcggtct gccagtagtt gtcgatgacc ggcacatcca
    28261 gcgtattgct cacccaactg gcggtcggct cgtccagcgg ttctccagcc agatagagca
    28321 cttccagcga cgagagatcg tgtttgcgaa tttcagcggt agggaatttt ttcagcacgc
    28381 gaatggcggt cggcgctgag aacatccggc taacctgata tttctcgacg attttccacc
    28441 acacgccgca gtccggccag gtcggcaatc cttcgtaaac gatagtcgcc atccccgcca
    28501 gcagcggcgc gtaaacgata tacgaatgcc ccaccaccca gccgatatcc gatgcgcaaa
    28561 agaacacgct gcccgctttg ccgccaaaaa tggtgtccat cgaggtcgcc agcgccaccg
    28621 catatccgcc gacgtcacgc tgcacgcctt taggtttgcc ggtcgtgccg gaagtgtaga
    28681 gaatgcagga ggtttcgttg gattccagcc acgccactgg tacccgcgcg ccgatgtgtt
    28741 gatagcgcaa cgacgcgaaa tcgacatccc gcccgctgac gcgcgccatt ttcgccagtc
    28801 cgcgatccac cagcaaaaca tggcgtggct ggtgctgcgc ctgacttatc gcatcgtcga
    28861 gcaatttttt atagggaatg attttgccac cgcgcgctcc ggcatcagcc gaaacaatca
    28921 gcaccggttt agcatcatca attcgcgccg ccacgctgtg cgaggcaaat ccaccaaaca
    28981 ccaccgagtg aatagcgcca atgcgcgcgc aggccagcag agtaatatgc gcttcggcaa
    29041 tcatcggcat atacaccagc acccgatcgc cacgctgcac gcccagcgag cgcagcattg
    29101 acgccaccgc gttcacttcg tcatgcagct gacgaaaggt aaaggtgcgc tcttcttctg
    29161 tttccgaaga gacggcaatc agcgccagcg cctctggctg tttctccagc cagcggtcga
    29221 tagcgttgtg gcacaaattg gttcggcctt cacaaaacca acgggcaaac ggcggattgc
    29281 tgtggtcgag cgtttgcgta aagggcgtct gccagtcaat acgccgggcc tgctcggccc
    29341 agaactgctc cggttcgtta atcgaacgct gataaaattc gctaaaagac ataatgctct
    29401 cctgttgaac ttacgcctta ccgccgttta cttaaatgac gtacaggtcg agatactcat
    29461 tgaccggcat ctgttccagg cgagttctgt cgagagaaac ctccagaatg cgctgctgtt
    29521 ggcgagtcgg gaactggcgc gcgagattga ttttgaattt atcgaccagt ttcggaatac
    29581 catcctgacg gcggcgagca tgaccaatgg ggtactccac caccacttct tcaaatcgtg
    29641 tgccgtcggt gaactcaagg gtaatggcat tggcgatggc gcgtttttcc gggtcgtggt
    29701 agtcagcggt aaatgccgga tcttcaaagc aattgatctt ctcgcgcagg gcgtcgatgc
    29761 gtttatcttg cgcaacgttg tcctcgtaat ctgccgccgt taagcgcccg aataacagcg
    29821 ggatcgccac catgtactga atgcagtggt cgcggtcagc cgggttattg agcggcccct
    29881 ttttgtcgat gatgcgaata caggcttcgt gggtgcgaat ggtcactttt tcgatatccg
    29941 ccgccgtctt gcctgctgcc tgcatctgtt catagagcgt catcgccgct tcaactgccg
    30001 tctgggagtg gaactccgcc gggaaggaga ttttgaacag cacattctcc atgacgtaag
    30061 aaccgtacgg acgctggaag cggaacgatt cacctttaaa ggagacgtcg tagaagcccc
    30121 ataccggcgc ggttagggct gacgggtaac ccatttcgcc cgttttcgcc atcagtgcca
    30181 gacgtaccgc gcgggaagtg gcatcgcccg ccgcccagga tttacgcgtg ccggtgttcg
    30241 gcgcatggcg ataggtgcgc agcgactgac cgtccaccca cgccagcgaa acggcgttga
    30301 gaatttcctc gcgggtcagg ccgagcattt cggcgaccac ggcggtggaa gccactttca
    30361 ctaacagaac gtggtcgagg ccgacgcggt taaaggagtt ttccagcgcg atgcagccct
    30421 gaatttcatg ggctttgatc attgcggtca gcacctgttt catggtcaac ggcgctttgc
    30481 cgctggcgac agcgttgcgc gaaagccagt ctgccgttgc cagaatgccg ccgaggttgt
    30541 cggaaggatg cccccactcc gccgccagcc aggtatcgtt gaaatcgagc cagcggatca
    30601 tcgcgccaat gttaaatgcc gcctggacag ggtcgagctg aaactgagtt ccggggacgc
    30661 gcacgccgtt gggtacgacg gtgccaggaa caattggccc cagcagtttt ttacaggccg
    30721 ggtattcgag agcttccaga ccgcagccga gcgtgtcgag caggcagtaa tgtgcggtgt
    30781 cgtaggccac tttagagctg atttcgtagt tcatcacgta atcgacgata tcaacgattt
    30841 cacgatcaaa ttccgggcgg atgttgttga tttgagctga cattgggtac gtttccttat
    30901 tgttattcgt agaggtttac tggcgcttat ccagcgcgac aaactggcgg tcttccggtc
    30961 caacataatt ggcggaagga cggataattt tgttgtcctg acgttgttcg ataatgtgcg
    31021 ccgcccagcc ggtgacgcgg gcgataacaa acagtggtgt gaacatctcg gtgggaacgc
    31081 ccatcatgtt gtaggaaaca gcagagaacc agtcgagatt ggggaacatc tttttgctct
    31141 cccacatcac cgtttccagg cgatcggcga tgttgtacat cttcaacgag ccgccttcct
    31201 gcgagagctg cttcgccaca cgtttgatca cctggtggcg cgggtcggcg atggtataaa
    31261 ccggatgacc aaaaccaatg accacttctt tgttttccac ccgcttgcgg atatcggctt
    31321 cggcttcgtc cggcgtttcg tagcgttgct ggatctccag cgacacttca ttcgctccgc
    31381 cgtgtttcgg cccgcgcagt gcgccaatcg cgccaataat cgcggaatac atatcagagc
    31441 cagtgcccgc aatcacccga ctggtaaagg tggaggcgtt aaactcgtgt tcggcgtaca
    31501 gcactagcga aatatgcatc gccttttccc agctttgcga tggcttttcg ccgtgcagca
    31561 gatgcaggaa gtgaccaccg atggagtcgt catcggtttc cggctggatg cgttcgccgt
    31621 tgtgactgta gtgataccaa taaaggagaa tcgagctaag tgatgccagc agtttgtcgg
    31681 caatatcccg cgcgccagag acagtgtgcc cctctttttc tggcagtgtg cagccgagcg
    31741 cggaaacgcc ggtgcgcatg acatccatcg ggtgcgacgc cgccggtaag gcttccagca
    31801 cggtacgcac attagctggt aaaccacgca gggctttcag tttcgttttg taagcggcga
    31861 gttcgtcacg ggtcggcagt ttgccgtgga tcagcaggtg cgccacttct tcaaattcgc
    31921 aatgttccgc cagatcaaga atatcgtagc cacggtaatg caggtcattg ccacttttac
    31981 ccacggtgca gagcgccgta ttgcccgccg gaacgccaga aagtgctacc gattttttcg
    32041 gtttaatgac atgggtactg ttttgcagga tcgttgtgtc gctcatattg tcctcgtcat
    32101 ttgtagggtc gagatatttt tcgttgtgtt ttatttcacc tggctacggg caaacaggtc
    32161 gtcgagcttc tcttcgtact ggtagtagtt gatgctttcg tacagctcgt tgcgggtttg
    32221 catggtatcg atgacgcttt tctgcgtgcc ttcctgacgc aggacgttgt agacatgttc
    32281 agcggcgcgg ttcatggcgc ggaacgctga aagcgggtac agcgccattg cgacatgggc
    32341 gctgcgtaat tcgtcggtgg taaacagcgg tgttgcgcca aattcggtaa tgttggcgag
    32401 gattggcacc tgcaccgcat cggcaaactg gcggtacatg gcgagttcgg taatcgcctc
    32461 cgggaacaac atctcggcac ccgcttcaac ataggcctgc gcacgctcga tcgccgcatc
    32521 cagcccctct accgccagag catcggtgcg cgccatgatc acaaaatcag gatcggtttt
    32581 cgcatccacc gccgcgcgga tccgatccac catctcttct ttcgagacga tcgctttatt
    32641 cggacgatga ccgcagcgtt tcgcaccaac ctgatcttca atatgcaatc ctgccgcacc
    32701 ggctttaatc atcgatttca cggtgcgcgc cacgttaaag gccgaagaac caaaaccgat
    32761 atccgcatcc accagcagcg gcagcgaaca aacgtcggtg atacggcgaa tgtcggtcag
    32821 cacatcatca agggtagaaa taccgagatc gggcagcccc agcgaacctg ccgccacgcc
    32881 accgccagaa agataaattg cctgatatcc ggcacgctgc gccaacagcg catgattagc
    32941 gttgatggtg ccaacaatct gcaatggatt ttctttagtc agtgcagcgc gaaacgcttt
    33001 acctggagag tgtagagaca tagtccatcc tttgttatca acttgttatt ttcgttaata
    33061 aaacaaagca aggggtgtgc cacaattgcc ttaataaaat taaatgttta atttcaattt
    33121 gttagagata acgtcatgaa ctaatccgca aatatgcgtt tcagttaacg tttcaggcga
    33181 tgtttcaccg cgtttcattg caacaattat gaaacaagac taaacccaat attcggtttc
    33241 ttaactttgc ggtgcgctat ggcacatcca ccacggctta atgacgacaa accggttatc
    33301 tggacggtat ctgtaacgcg cctgttcgag ctgtttcgcg atatcagcct cgagtttgat
    33361 cacctggcga acattacccc tattcagctt ggctttgaaa aagcagtgac ctacatccgc
    33421 aagaaactgg caaacgaacg ctgtgacgcc atcatcgctg ctggctctaa cggcgcgtac
    33481 ctgaaaagcc gcctgtcagt gccagttatt ttgattaaac cgagcggcta cgatgtgtta
    33541 caggcactgg caaaagccgg aaaactcacc tcttctatcg gcgttgtcac ctatcaggaa
    33601 accattccag cgctggtggc gtttcaaaaa acctttaatc tgcgcctcga tcaacgtagc
    33661 tacattaccg aagaagacgc ccgcgggcag attaacgagc tgaaagctaa cggcactgaa
    33721 gcggtggtcg gcgcggggct gattaccgat ctggcagaag aagccggaat gaccggaatt
    33781 tttatctatt ccgccgccac cgtgcgccag gcgttcagcg atgcgctgga tatgacgcgc
    33841 atgtcgttac gccataacac tcacgatgcc acccgcaacg ccctgagaac tcgttacgtg
    33901 ctgggcgata tgctcggtca atcaccacag atggaacagg tgcggcagac tattttgctg
    33961 tatgcccgct ccagtgcagc ggtgttgatt gagggggaaa cggggacggg caaagagctg
    34021 gcggcccagg cgattcatcg ggaatatttt gcccgccacg atgcgcgaca gggcaaaaag
    34081 tcgcatccgt ttgttgccgt caactgcggg gcgattgccg aatcgctgct ggaagcagag
    34141 ctgtttggct atgaggaagg ggcgttcacc ggctcgcgac gcggaggtcg cgccgggctg
    34201 ttcgaaattg cccacggcgg tacgctgttt ctggatgaga ttggcgaaat gccgctacct
    34261 ttgcagactc gcctgttacg ggtgctggaa gaaaaagagg ttactcgcgt cggcgggcat
    34321 cagcctgttc cggtggatgt gcgggtcatt agcgccactc actgcaatct ggaagaaaat
    34381 atgcggcaag ggcagtttcg ccgtgatctg ttttatcggc tgagtatttt gcgtctgcaa
    34441 ttgccaccac tgcgcgagcg ggtggcggat attcttccgc tggcggaaag ctttttgaaa
    34501 gtgtctctgg cggcgctctc cgccccgttt tctgccgcat tacgccaggg attacaggca
    34561 agcgaaaccg tgctggtgca ctacgactgg ccaggcaata ttcgtgaact gcgcaatatg
    34621 atggaacgac tggcgctgtt tttaagtgtg gaaccgacgc cggatttaac gccgcagttt
    34681 atgcaactgc tactgccgga gctggcgcgc gagtcggcga aaactcccgc tccacgctta
    34741 ctgacaccac aacaggcact ggagaaattt aatggcgata aaacagcagc ggcgaattat
    34801 ttaggcatca gccggacgac gttctggcgg cggctgaaaa gctgaactgc cgaatgcagc
    34861 gcgaacgctt tatccggcat aaatttcaat agatgatgat caccgataaa tttacgcatc
    34921 ggggatgttt acataggttt cagaattact tcttcttata aatatttgcc gtgccgtgga
    34981 tcttattgtc agtttgacca gaggtcagta ccaacacatc agcccctttt tcatccgctt
    35041 ttttgatcaa atcctctttc gcatctgcgg tcgacatttc attactggtt gaaatatcac
    35101 ctattttttc atactgcgat tcaacttttt caaattccgc tttggtcatc aattcagcgg
    35161 catagacatt ggtaacggca aacgctaacg caccgactaa cattttagag attattttca
    35221 tattacggct ccagagttgt tatcacacta tcacccaccc acattattgg gttgattaat
    35281 aattagttca gtcagggcat aaacgccagt aaccggaaat aagacacttt gtgatctctc
    35341 tcgcacccga ttatttttgg tagaaatatg atgaagttac ttcacttatt tatggatgaa
    35401 atcactatgg atcctttgca cgccgtttac ctgaccgtag gactgttcgt gattactttt
    35461 tttaatccgg gagccaatct ctttgtggta gtacaaacca gcctggcttc cggtcgacgc
    35521 gcaggggtgc tgaccgggct gggcgtggcg ctgggcgatg cattttattc cgggttgggt
    35581 ttgtttggtc ttgcaacgct aattacgcag tgtgaggaga ttttttcgct tatcagaatc
    35641 gtcggcggcg cttatctctt atggtttgcg tggtgcagca tgcgccgcca gtcaacaccg
    35701 caaatgagca cactacaaca accgattagc gccccctggt atgtcttttt tcgccgcgga
    35761 ttaattaccg atctctctaa cccgcaaacc gttttatttt ttattagtat tttctcagta
    35821 acattaaatg ccgaaacacc aacatgggca cgtttaatgg cctgggcggg gattgtgctc
    35881 gcatcagtta tctggcgagt ctttcttagt caggcgtttt ctttgcccgc tgtgcgtcgt
    35941 gcttatgggc gtatgcaacg cgttgccagt cgggttattg gtgcaattat tggtgtattc
    36001 gcgctacgcc tgatttacga aggggtgacg cagcggtgag cagataattt actctctacg
    36061 taatcaacct gatttgccga atcccggcca tcggcgatga tagcccccgt aatgcgtggt
    36121 ccttatttcc gataagttta cttgagtctt tagcttccca tttcagccca ctctcccctg
    36181 tgtgggcttt tttcattggt ctgacccgtc ctgaatagcc aaacaatggc aatgttccac
    36241 tttgcaggga tgataataag gggatctgat tcagaagagt tttgaaaagt tggaccgcca
    36301 tcgaggcctc gaaccccgta ccatacaaca atcctggttg tcgtctgctc ttcctgatga
    36361 gctaatggcg gtttttgata tgagccttac agcatatttt ctgtcaggct tgagtgcgat
    36421 gcgtaagata cagaaaagac cccacacaaa catgatggaa ttatgatttt tttcatatga
    36481 atgtcaaccc acacattcac aaaacatctg catgagatca ttttttatac accgcatgac
    36541 tgcgaaacca ctactggtgc ttttttaagc gaagtgtcgc cgatcacaac aaaatatcgg
    36601 cgaaaaaaga gatggaatgc cagttggcat acagatatac agatgaaatg attacctgca
    36661 ttgcgattca ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga
    36721 gtgtagcgga ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact
    36781 ctattcgata tccattcatt taatcgtgcg tcatctctgt atgtttttac gtccagaaca
    36841 gagagcaata acatcaccag tgattcagga aaaacagggt ggatattttt taagtgtaat
    36901 aataatccat gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt
    36961 tcgcgaagcc atttttccac acgcatacac tctttccctt tagggaaaat tctcagcaaa
    37021 ggcatatggt gttcaagaag aatacatttg caatctgttc gcactgaatc gataaactgc
    37081 actaatttct taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac
    37141 gcatcatcct ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca
    37201 aacttaaccg ggtgatcagg gtaatccatc cagtcataaa atccagggaa atcattaaac
    37261 tgcacattta tggtgagtgc gttatcgtct aaccactcac agtctcgtac ggcagccatg
    37321 agatgaatga gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa
    37381 tattgtttaa tcaaaacctt gaatatatcg acacaggaaa ccaaggagct tttgatatcc
    37441 tctggtaact gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa
    37501 ttattgtgcg gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg
    37561 cagataataa agctctcatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag
    37621 aagtattgtg tactggaggg agttaattta acaatttgaa tttacgttat aggttttact
    37681 gctactttga tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat
    37741 atgttaaacc acagggtatt tattaatttt ttcagtctgt tagtgtgcga ttatcgataa
    37801 caaaacgata tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg
    37861 cccgaatcat ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct
    37921 gagtttctgg aatgccgcca atcatagaac cggctatcgc acggcgtttc atgatcaggt
    37981 tgaaaacttc cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat
    38041 cacgcttcag caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa
    38101 tgaaatcgaa actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt
    38161 catcggcccc cagggctttt gccgcttcgc gttttgcctc agaagtggta aatgccacca
    38221 catgtgcccc catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga
    38281 ccacgcccac ttttttaccc ggcccggcct gccagtgacg tagcggcgaa tacgtggtga
    38341 tccctgcaca caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca
    38401 gaacatatcg ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt
    38461 cgtccggcgt cggcgagtta taggtgccgg tcatgtgatc acagtagttt tccagcccgt
    38521 cttcacactc ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc
    38581 ccggcgcatg tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac
    38641 ccggtacgca ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat
    38701 ggcagacgcc acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg
    38761 tgatatccat cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca
    38821 ttgtgtttac tcctgattag ctatgtgtat ttggcgatgc ctgatatgtc atcgcggcaa
    38881 aacgcgtcca ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg
    38941 aacctgaatg aaacagagca gcaacgttcc ccataaggcc atcgtcaggt aggggcttat
    39001 accgagaata ttgaagccgc tttcaataat ttgcagaaga aacagcgcca gcaccatacc
    39061 aattatccgc ccgctgccgc cgtccggatt aaccccgccg agtactgccg cgaggattga
    39121 caccagcaag tacgattcgc cataagaggc cttagccgag ttgagtttcg acatcatcaa
    39181 aaatgccgct accgcacaaa gcaaggcgga aatgacataa acccaaatca gcacccgacg
    39241 agtgttaatg ccggagtaaa gtgttgctcg ttcgttagac ccaatcaggt aaatggcttt
    39301 accgagcggc gttttctcca gcaatatcca cagccccagt gccactgcgg caaacaacca
    39361 catcggtagc ggaataccga accattgcgc atgattcaac cacagcaccc atgagggata
    39421 attggcaatg gcgctaccac ctgtcaccag aatatttatc cctttgagca acgtcatcat
    39481 cccaagcgtt gcaagaattg gcgacactcg aataccagca atcaatacgc cgttacataa
    39541 accgattatc accgccgcgc cagcgcccgc cagtaacgta gcgacaaccg tagcaatacc
    39601 cggtggatac tgagtcgcca cccacgccat caccagtgaa caggcgttag cagtggctat
    39661 aattgacaag ttaatgccac cgcaaagcat ggtcaccgcc atagccaacg ccagaatgcc
    39721 cagcacgggc atttgcgaag cgacggactg gaaattactt atcgaccaga aaatctccgg
    39781 catagtaatg ctgaacgcgg caacagttag caccagcagg ccgatcaagt aaaactcaac
    39841 gttattacgc cacgattttt tcatagcaga ctccggttct gatgctgact ccacgccgtg
    39901 gcactaatgc tggcaacgat gatgatgccg gtgatcaatg tttgccagta agacgagact
    39961 cccaataaat ttagcccgtt ttgcatcact gccaacaaca ccacgccgag caaagtaccg
    40021 gttaacgtgc cgcgcccgcc gagcaaacta gtgccgccaa gcaccaccgc agccagtact
    40081 gtcagctcat aacccagaag agaatcgggg gcgacagtca tcaccgtcca cgactgcact
    40141 acacccgcag cgccagacat caatcccata tagccgtaga cgaaaagttg cagtttcagc
    40201 acgctaaagc cgatgcgact ggctgattcg cggttcccgc caagggcata aattttgcgc
    40261 cctacactgg tgaaattcat gataaacgct gtcagcagca ccaccgtaat catggcgatc
    40321 agcggcagac caaggccata gtcatagcca tcagcatcgg tgtacttgaa caacataacc
    40381 ccctgctcaa accacggcgg aaagtcgtac agccacacac ctttactcaa ccataacagc
    40441 aggccataga aaatattcag cgtcgagata gtgatgatga tagaaggcac ccgtaggcga
    40501 ttaaccagta aggcgttaat taagccaagt aaaatgccga tgcagcccgc cagtgcaaac
    40561 gcgacagcga agttgccacc aattttttgc agcaacagca ccatgccgta ttgcgcaatg
    40621 atggtcattg ctggaaacga aatatcaatt ccaccggaaa tcaacaccac aaacaaacca
    40681 caggcgagaa tagtcaacat ggcatagtta ttggcgagat cgtacaaatt accgaatgtc
    40741 agaaactcgt cgctgcgcca ggcaaggcca acaaacagca ccacaatcag taatgccagc
    40801 cagaattcat ggcgtttttt cagttcagcc atttaccacc tccgcaatcc tggcctgaga
    40861 actgctgtca ggcaggaagc tatgggtgat ctgacctttt tgcattacca aaatccggtg
    40921 gctttgatac cacgcttctt caatttcatc gcagatcatc aataccgcaa taccatgagc
    40981 cgccagatcg ctgataatgc cgtatattcc tgctttgttg gcgatatcaa caccgactgt
    41041 cgggctatcg agaatcaaca gtcgtggccc aatcgccagc catttggcga tcgatactcg
    41101 ctgcgcgtta ccaccagaaa gcgtattcac cggaagatga ggatcagaga ctttgatggt
    41161 caatgccttg attagcctgt ccaccaggtc acgaacttta gtttcactca gaaagccgaa
    41221 gcgatctttt accttgtgaa aaacagtgct aatgatgttg tcttcaatag attgcgccat
    41281 caccaaaccg cgcgacatgc gatcttccga aacataacca ataccgtggc ggatagcgtc
    41341 ctggttacta tgcagtgtaa ccggctggcc gttgatgagg atctcgccag catccggtcg
    41401 ggtcatacca aacagactca gacataattc cgtgcgcccc gcaccaagca ggccaacaat
    41461 cgataccact tcgcccgctt ccacccgcaa gttaatattt agatattccc catgacgact
    41521 taaattacgc acctccagca ccggggtgct ggattttccc tgccacaact ctcgcacctg
    41581 atattcaaac tcctggccgg tcatcaggaa accgagttgt tttgtggtca tctctgcagc
    41641 cggaaaagtc ccgaccagct caccatcttt cagcactgaa atacggtcgg aaacttccat
    41701 cacttcttcc agacgatgac tgacgaagac cacgcagatc ccgcgttcac gcaattgatg
    41761 caccacctgt aataaccctt ccacttcctg atgggtcagc gaagcggttg gttcatccat
    41821 cacgatcaac cgtgcgtcct gcgccagtgc acggcaaata gccaccagct gacagcgggc
    41881 aattgaaagc tcagaaacga gtgtatccag tggcaatgtg acgttaatac tggtcattgc
    41941 cgcctgcgcc acctcacgca gacgacggcg gttaacaaac agaccgtggt gatagtgatt
    42001 cacggcgata ttctcccaga cactcaggtt aggaaataaa gagaggtcct gataaatcac
    42061 ctgaatcccc tgcgccaccg aagcggcggg tgtcagcttt gaccagtttt tccaaccaag
    42121 cgtaatttcc gctccctcat ccgggcgata aacaccggac ataattttaa tcaacgtcga
    42181 tttaccacag ccgttttgcc cggcaaggca gtgcacttca cccgacataa agtcgagaga
    42241 aacatcgcgc agcgctcgcg tggcatgaaa cgttttgttg atatgacgaa gggaaaggaa
    42301 agtttccata acatcccctg gcaataagaa tcggggcttt cgccccgaag aaatggatta
    42361 ataaagtgag tcgatattgt ctttgttgac cagtaatacc ttatggaaac ggataattcg
    42421 cttatccata tctacgtcgg ccttacccag attctgcatt tctaatccag gtttgatctc
    42481 ttcacccttc agcaacgtgc tggcgacggc tgcgagtgcg taacctgcag aggccggatc
    42541 gtaagtaatc ccttcggtga tatcaccact tttaatcagt gatgccgcct gtgaagggat
    42601 catcatgcca tagactgcga ctttattttt cgcccgtttc tctttcaccg cacgtcccgc
    42661 gccaatcgga ccgtttgaac caaaggagac aaccgctttc aagtcaggat aggttttcat
    42721 caggtccagt gtggtacgac gtgagtcatc cacactctcg gcaaccggca tgcggcgggt
    42781 aacttcatgc atatccgggt aatgctcttt ctggtatttc accagcaagt cagcccataa
    42841 gttatgctgc ggcacggtca aactacccac gtaaatcaca tagccgccct tgccccccat
    42901 gcgtttcgcc atatgctcaa catattcagc ggcaaatttt tcgttatcaa tgatttcaat
    42961 atcccagtta gcacttggct gaccggggga ttcgttggtc agaaccacaa ttccggcatc
    43021 tcgcgctttt ttgaataccg gttccagcac gttggcatcg tttggcacga tagtaattgc
    43081 attaacctta cgggcgatta aatcctcaat aattttaact tgttgcggag catcagtact
    43141 tgaaggcccc acctgtgagg cattaacacc aaaggcttta cccgcctcaa ccacaccttc
    43201 gcccatgcga ttaaaccacg gcataccatc gactttagaa atattcacca cgactttttc
    43261 cgctgcctgg agcggcgcag aaattagcgc agcgcctaat aacagcgaag acaccatatt
    43321 gataacaaaa cgtttattca tcatttcagc ctcattgcag ggtagtcagg aagtgttcgc
    43381 ccaaattagc gcgctggaga gaataagaat tttcgaatat ttttatgcag aataaacagc
    43441 cctatttcag gcagctatta aaaatatggt tataaatatg gacaaaacgg gcagcaacaa
    43501 taagtgtgac attcatcacg taaaaaatag caaaataaac aaaattagcg atattttatg
    43561 tgtgtgagtt tatttcgcgt agataaatag ttatctacac aacgtcaata agggcatatt
    43621 cttcaaatat aacgtccatt tctggtgaat agcttccttc tgcctggaaa aaacgacggt
    43681 gttcattacg ccaatattca agactgaggt caccttcacc ttccttacgg gcaagctcca
    43741 cggtgacatc agaaaagcgc agtaaatgca ggccaatcac tctgataaca catacaggct
    43801 caccacggct attttctaca atcttatact caccaatcat cggaaatgcg ttgtcttcaa
    43861 tacatcccgc cagcgaacca caggaagctg ttttaacacc agtcgcaatc aacgttgcga
    43921 ggtgatcggc caacgctgct gaatcgccaa aagtacaaaa ggaagcatgg ggatattttt
    43981 gtgagaattt ttcttttatg gtcatggcaa tatttttgcg attacggaaa aacaaatcac
    44041 atcttaatta ctgttggtaa ccatcagcaa gagtatcatt ggtttttcga tactgaatct
    44101 tttttatccg aagcaggtca catttatggc ctgataaata cctttgttaa aactcacttt
    44161 tgtgtatttc tctcaaataa tcccctggaa attatgccgc gagattataa agttcaggat
    44221 cattcattag caaaccattc ccgccccact ccctggggct taaatctctt acaattcacc
    44281 gttgcaggcg tttcaaatgc gcatcaacct gccacactcc cccacaccag ttgcccttta
    44341 tggaacgttg cggttctcgg cgagatacgc gccaccgcct cggcggaaca ggaggcgtcc
    44401 accagcacaa agctggcatc atcctgcgct tttggccata cacgctcgcc tttttcattc
    44461 agcggcaata catcgccagt ggctagaaac agcgaacggg agaggttctg ttcgttagga
    44521 cgaatataga gctgcgcgta gagattggct ttttccagca tgtcgcccag accataaggc
    44581 gaccagtggt cgataacgct gtcagtgcca gtcatcactt ttacgccttt gtcgtgcaac
    44641 tgtttgagcg gcatatgcag cgtgccaatc ggcaccgtcg aggcgataga aatttgctgc
    44701 gccgccatcc gattcgccag cgcatcgacc tgttgttcgt tgagcgtagc cagcgcaaag
    44761 gcgtggctga tggtcagctt acccttcagc tgtggcgttt tctctaccgt ttcaaccata
    44821 taattgatgg ctgccacgcc cgacggagtg gtttcgtgca ggtgaatatc gacgcctttg
    44881 tcgtagtcca gcgcaatctg gaacatggtg tcgagggatt tttccatcgc gccatcaaca
    44941 ctggtcgggt ccaggccgcc gacgtaatgc gcccccgcct gcatcgcttc acgcatcaaa
    45001 ggttcagatt tcgacagcag caaaccgtgc tgcgggaagg cgacgatttc acactcaaag
    45061 cccgcctgac gtcgcgccag caccgcctgc aaattttgca gatttttcag gccggaaact
    45121 ggttcgatat tgcagtgact gcgggcaatg gtggtgcctt tcgactgcaa tagatcgatc
    45181 agtttttccg cccgctcctg cgtatacggt tgtaactccg gcagcatttt ctgctcgagt
    45241 ttgatcatgt cctggatagt ggtgcctgcc ggacgattga gcgagcgcca cggcccgccg
    45301 tagaaggttt tgtcgagatg aatatgcatg tcgcgagtgg tgggtagcat caacttaccg
    45361 ccagcgtcat agtgcggcag cgtggcgtcc ggatgctgct tgttctcgcg cagggcgaca
    45421 attttgccgt cctgaatctc cacggtctgg cgcgccgtgc gggtctgcac cgccacaccg
    45481 ttttcgtagt caaaaccggt ttccagcagc acgttatcga gatagtaatg cgggtcagtg
    45541 attttcatgg tgttgttcgc ttcgcagttt agggtgccag ctaccgccgc atgggcgagc
    45601 ggcacagagg taccaaacag cgcggcggcg gtgaccatct taccgctctg gctcagaaat
    45661 tcacggcggc tattgctttc tttcatccta cgtccttacg ttttaataat atgggtgccc
    45721 gtttcaccgc gcagcgccgc aggcaggcat tccggcgtgg tgataatcac ttctttgccg
    45781 ccctgctcca ggaacgtcag gctggcgatg atttttggca acatgctgcc gggcggaaaa
    45841 tgcccttcct gcatatagcg agtcatggtg gcaatatcca cccgatccag cgcctgctgc
    45901 tgcggtttac caaagtggat acagactttt tcgacgccgg tggtgatcac cagaatgtcg
    45961 gcgtgaattt cacgggccag cagcgcggta gagagatctt tgtcaataac cgcgtccacg
    46021 ctttggtaat cccccgcttc agtacgcact accggaattc caccgccgcc cgcgccaatt
    46081 acgacaaaac cttgttggat cagcgcctta atggcgggtg cttcgacaat acgtttcggt
    46141 tccggcgagg cgaccacgcg gcgatagccc cgtccggcat cttcaacaaa acaccagtca
    46201 gggtttgcct tttgtagttt gtcacgctgg ctttcactga agaatgcacc aatgggcttg
    46261 gtgggatggg caaaacctgg atcgttttta tccacttcca cctgagttac cacggtgacg
    46321 gcttttttct cgccgtgacg cgccagccgg ttattcagcg cctgttggat cagatagccg
    46381 atgccgccct gcgtatctgc cacacagttc gccagcggcg ttaagggcag cccttcgcgc
    46441 tcgtgggcaa tctccgcacg gcgtaaatcc agcccgacct gcggcccgtt accgtgggtc
    46501 agcacaatgt cgtaatcgga agccagcatt tccagcaccg tatcggccac ggctttcacc
    46561 gcctccgcct gatgctcaat cgactggctg gcgttatctt tgataatgct gttgccacca
    46621 atggcaacga ccacaagctc tttcatgttg ttatgtccgc tacagagggt gattaggtta
    46681 tgaccaacgc cagactgcct gatgcgctac gcttatcagg cctacaattc tcgggccatc
    46741 cgtaggccgg ataaggcgtt cacaccgcat ccggcataaa catgtaagtc agacgccgta
    46801 acgttcgcac cagccgagaa tggctttctc gaagcaggcg aatggtggat gcacaatgcc
    46861 cgcgccaatc atgccgacgc ccgcatcttt atgggcgatg gcggtgttga tcaccggcag
    46921 aatgccgctg ctacccacgc gggtgatgtc gattgccgac ggcacgccca taaaaccgag
    46981 cagcggaatg gtgacgttgg ggttttcgcc aagggtgatt tcgcgcatct gacgggagaa
    47041 atcgatagcc tcttccaccg tgccgcccac cagtgcgacg attgcaggcg cggtcgccat
    47101 ggcaaacccg ccaataccgt aggtttctgt gatggcgctg tcgccgatat ccagccccga
    47161 atcttccggc ttataaccag caaacatcgg gccgatgacc tgctgcgctg ggccggtaaa
    47221 ccactgcccc ggcaggccgc tgacccgcag gccgaactcg acgccgttac gcgccatggt
    47281 ggtgaccacg gtgctgtatt cgatgccgtg cgccgcatcc atcgccgctt tacacatcgc
    47341 catccacgtc gggccggaga agtagtcgct gctggcgaca aactcgaaca cttcgcgctg
    47401 ttgctcgacg gaatagcctg cctgaatgat ccccggcgtc agtgcctgaa tcagcagcgt
    47461 cgtcccggcg ttattgcggt tatggcactc atcgcccata tgcagcgcct gcgccagcat
    47521 taagcgcaga tcgatctcac cgataatttt catcgcgtcg cgcagcattg gcccctgcac
    47581 atcgcgcatc cagttgaggc ggtcgatcac gctctgatcg ttggccccca tacgcaaaat
    47641 cttcgccatc tgctcgctca tattggtata agcgatgttg ccgtaggttt tgtttttcac
    47701 gatgtgcata aacatcgagg ccgaggtaac gcccgccatc gatcccacgc agtcgtgttc
    47761 gtggcacggc gagaaggtga tctccccgga ggccgccagt tcagtcgcct catcgagatc
    47821 tttcgccagc ccttcgaaca ccaacgcccc ggtgaccgcg cctttcatcg cgccgcacat
    47881 tttttcccag gtgatcggcg gcccggcgtg aagaatggtt ttcggcgtca tgcccggcac
    47941 cacgttaatc gcctgatcaa aaccaatcag caccggatgc gactggataa tgcgctccag
    48001 cgcctgctgg ttagcggcgg cgattttgtc tgccagcggc gaatcggcaa tgttatccag
    48061 cgcctgcacc acctgcatat tgccctgccc cggcggcgtc cagtcgagtt gagtcacttc
    48121 tacatgctgc tttttcaggt catcgctaaa catggcgata ccgacgttaa taacgttcaa
    48181 cggttggcta aacagtgact ggctcatcag gcttcctccc ctttgcagat aaattcacgc
    48241 gccagcaatc cggtattggt gctgctgctc gccagaatca ctccggcatc aagcagcatc
    48301 tggctttgtt gttctaatga tggcgtatcg agatcggtgc ccagcacata ggcgagaatg
    48361 atcaactcgc gtccttcggc ggcggcgatc gctttcgctt ctttgatcgc ctcgatggtg
    48421 gagccgaccg gatcttcatg cgatccaaat ccgagcacaa aatccatcac gatcaccgcc
    48481 acttctggat cgcgcgcttc ttcgatcaag cgactgatgc ggttggtggg gtcaatcatt
    48541 ggatgcggct tgccgttggt gaagtcgtca tcgccaaagt cgaggaaggt gtgtttgatg
    48601 ctgcggttga tatcttgcag gcggaattca ggatcgggct gaatgttgct gtagacatcg
    48661 ccatgttttt ccatcaccgc gaacatggtt tcgtcgcaca gcgtgccgcc gcagaacagg
    48721 ccacgaatgt atttctgctg cggttgcaga cgcgcacgca catcagcaat caacggctgg
    48781 ttaagcgtat gcaggtcgag attttcctgt ttcacgccgg agagcatcac cgcttttagc
    48841 gccgcctctt tgctgccgcg ggcaaactgt agcccctgct catccaccgg cgtttcaacg
    48901 cgaccgagga agcagaccac caccggcttg cggcaggcac gtgcgcgttc cagcactttg
    48961 cgggccaccg caggcgcggg aggttttgaa atcagtgcga taatttcagt ttgcggatcg
    49021 ttttccagca tcccgatggc gtcgagcatc atcaggccgc cgattttctc gctcaggtcg
    49081 cgcccgccgg tgccaatcag ttgtgaaaca ccgccaccaa attcatgaat gcggacgctc
    49141 agctcctggc tgccggtgcc ggatgcgcca acaataccga tattgccgcg acgcacggcg
    49201 ttaccaaaac agagcgccgc gccgttaata atcgccgtgc cgcagtccgg ccccatcatc
    49261 agcagccctt tttcgtgggc caactgcttg agcgccagtt catcttcaac tgagacgtta
    49321 tcggaaaaca gcatcacgtt aaggtcgttt tgcagcgcct gacgcgcttc gcgagcggca
    49381 aacagaccgt taaccgaaat caccgccagg ttactttccg ggatatgctt tttggcgctg
    49441 gcaatagtgg cgtaacgcgc ctcgtgcgag ccgctttgcg ctttgctgtt gaacagttct
    49501 tcaatctcca ccagcaactg ttcgttgtcc gcgcccgctt tgccgttgat gacaatcatc
    49561 aggtcgccgt ttttcgcctg ttccagctcc ggcgtcagca gtcctaaatt tttcagtaca
    49621 cctttgttca tttcggtcgc catcgccaca aatgcctgct cgacgccgtc gagtttattt
    49681 gcacgcgtgg agatagacat cagcgagaca gaatcaaaat aggtattcgg tttaatgact
    49741 attttaactg acataattcc ctccgtaggt ttggctcagc gcacaaccat cggccatgcc
    49801 atacagcatg tcgcagccgg aactggagcc aatatgttta attttttcta ttgcctgagt
    49861 tgcgttccca gggatgtcat aaataatgtt gtgaataaaa cgatgaatat tttcccggca
    49921 acgttgttgt aatgcggctt ccagcgttat agcacttaat aatgtggtgt tatttctgcc
    49981 gcgctgcata ccgagataaa attcctcttt gtatttttcc gacggatgcc cgggaataaa
    50041 taaaataagc gccagacctg ttaaataatc gtcggctgat ggcgtaaggc caatccctaa
    50101 cccaatcata ctggcgaccg ctgcggcgat attttgtttt tcgtttactg cctgaataac
    50161 tgcccgtcgt ttaatatgta gttctttatt taacgcctga taaaacggat tatcgccgcg
    50221 ataattaaac agggtttcat tttcccccag gtgctgatga ataatgtcgt gccactgtag
    50281 ccaggcgata aggtgaaaat tcacagcggt catttgcaag gttggggaca gccaacgccg
    50341 acaacgactc atctctatat gaagatgttg accaaccgta ataccttgat cgtcaaactg
    50401 aaccttatca ccatgccgga acagatcatc aaagtgagtg agtgccaacc gacaactgtt
    50461 tggcgcattg tcgtactctt cacaaagcag cgtcagcaac tgctggcgag ccgggagtgc
    50521 gatatttacc gctctggcaa agacctgttc aacctgtcct tgcccccgct gcgccagaaa
    50581 atccgcatcc gcagttagcg cccacacaca ccgtctttct ggtgtgttga tagcaagcct
    50641 cccggtttat gcacctgcgg caatcagtaa ctgcgcaatc tcttcaaagc cccgttcccg
    50701 cgccagttcc agtggcgttt tgccatattt atcggtcaga tgcgggctgg caccgtgttc
    50761 cagcaataac tgcacaatcg cctgctgttt aataccacca tcattaagca caatcgcttc
    50821 cagtagcggc gtccagccta catggttggt ctggttaacg ttaatctccg tatgcgccaa
    50881 aagctctttt acaatactta aatggccttt ttcacaggca ggcgtcaggc cgacaccgcc
    50941 aaaacgggtt acgcaattaa gatcgggttt agccggtaaa ataattcgta gtagcgttaa
    51001 atcatcgttc agacagctaa ttaaaaaagg atttaaacag gtatgatctt gcttattaat
    51061 atccgctccg gcatcaatta atgcctgaac gcaagcatat tgctgatata aacttgccag
    51121 cgtaattgcc gttttcccct gacgatcgca ggtattaata tcgacaccca gcgcaaggca
    51181 ggtttttact ttatcgatat caccctgttg tgcagccaat aaatagtcgg caggtagatt
    51241 ttttatactc attcacattc ccctggtttt tgtggctgtt ttcgatgaat tgagaatagc
    51301 aacgataatc tgataaaaga acccgcttaa atttaattcg ctgtatttcg cctttttgtt
    51361 gaatgatgtt caacaatctt ttcgcagtgt gcggtagtgc aatatttgtt tctttgaccc
    51421 gatacccttt cactcaatca ggttttattt ctctatagtc tgcacatgta agagtagctt
    51481 tcgtccatgt ctaaccagag gggaacaaga tgaacggact cacggcaacg ggtgtcaccg
    51541 tcggtatttg tgcagggctt tggcagttag tgtcttcaca tgtcgggctc tctcagggat
    51601 gggaattatt aggaaccatt ggcttcgtcg ccttttgcag tttttatgcc gcaggcggcg
    51661 gtaaatcggg gtttatcaga agtctggcgg ttaattattc cgggatggtg tgggcgtttt
    51721 tcgccgcgct gactgccggt tggctggcct cggtgagtgg gatttctggg ttctgggcga
    51781 gcgtgattac taccgtgcct ttctcagcgg tagtggtctg gcaaggacgt ttctggttac
    51841 tgtcattcat tcccggtggc tttctcggca tgacgctgtt ttttgcctgc gggatgaact
    51901 ggacggtgac gttactgggt tttctggcgg gtaactgcgt ggggataatt tcagaatatg
    51961 gcggacaaaa attaagcgaa gcgacgacta aacgcgacgg gtattaaaag ttattcaaaa
    52021 atgattatct atctttaatc gatacctcta cagtcatggc tggagaaatt catattagat
    52081 aatggcggga atggtttaat gaactcagtt tttaccgaag agaatctgtt ggcatttact
    52141 accgctgcgc gttttggcag tttcagtaaa gccgcagaag agttgggttt aaccacttcc
    52201 gccattagct acaccatcaa gcgtatggag acggggctgg atgtggtgct gttcactcgc
    52261 agtacccgca gcattgagtt aacggagtcc gggcgttatt ttttccgcaa agctaccgac
    52321 ctgctgaatg atttttatgc catcaaacgt agcatagata ctatttcgca gggcatagaa
    52381 gcgcgtgtgc gcatttgtat taatcagctg ctttatacgc ccaaacacac cgcgcgctta
    52441 ttacaggtgc tgaaaaagca gtttcctacc tgccagatta ccgtcaccac cgaagtgtat
    52501 aacggcgtct gggatgcgat tattaataat caggccaata tcgccatcgg cgcacccgat
    52561 acactactgg acggcggcgg tattgattac actgaaattg gcgcgattcg ctgggctttt
    52621 gctatcgccc cggatcaccc gttagccttt gtgccggaac ccatcgccga aagccaactg
    52681 cgtctctacc ctaatattat ggtggaggac accgcgcata cgattaataa aaaagtgggc
    52741 tggttgctgc acgggcagga gtcaattctg gtgccagatt tcaacaccaa atgtcagtgc
    52801 cagattttag gtgaaggaat tggttttttg ccggattaca tggtccgtga ggcgatgacg
    52861 caatccctgc tggttacccg acaaatccat aatccgcgcc aggattcgcg catgttactg
    52921 gcgactcagc attctgctac gggtcaggtg acgcagtgga ttaaaaaaca atttgcgcca
    52981 aatggcatat taacggggat ctatcaggat ttactgcatc gggagaatta atcgctgcca
    53041 acctcccgat gcgctgtgaa tagcggttta catcaaccac ctgctttcat tacccattca
    53101 gagataaatt tattacccgg tactggcgga gagtacaaat agccctgcaa aaagtgaacg
    53161 cctttaccga tcattaaatc cgcctgctcc tgggtttcta ccccttccgc cacgatactc
    53221 agaccaggct tacgcgctag ttcgacaata ttgtccacaa tatgaccgga gatttcgtcg
    53281 acactcgcca tttgcacaaa tgacttatcg atcttaataa aatcgaccgg gaacgcctgc
    53341 aagtaacgat aggtcgcata acccgtacca aagtcatcca gcgcaaaggt aatgttgtgc
    53401 tgatgaaggc tgtcaaatat cgctctggct tctggcgtta ccggaatagg gttacgttcc
    53461 gttagctcga gaaccagctt gattttatcg ttacctaatt tcttaaccag gttcagacac
    53521 tctttttcaa atcccgctgc caaaaaacaa cccgccgaga cgttgatgcc aatatggaaa
    53581 ttgtccggca gcaaatgttt taccggcatc agaatatccg cagtctgttt catcagttgg
    53641 cgggtcatta tgacaataag accggatgac tccgccagag gaataaactg atccggtggg
    53701 ataattcccg tttgtggatg ttcccagcgg acaagtacct cacagcccgt cagtacccca
    53761 gtctgcgcgc agaaaaccgg ttggatccac ggtttgaatt cgtggttttc caacgccaga
    53821 ctgatggctt caggcgtgac gatatggtga caacggttat cagggatata aaaattattt
    53881 ttatttcccg tgccactgat gacttgcgga tgatattgaa aaaagatatc ccgccagaag
    53941 gtaatatcac tctggatgcc tagtttttca tacagctgct tcttttgatg tgaaattgtc
    54001 tttgcactgc gatttctgta ctgtgagatt tctgtaaccg acattccctc cattagcagc
    54061 ttgagtaaaa gccgttcttt ttcggacacg ataccaggca acgtcaaatt caccgttgcc
    54121 ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca tcgaacacac cttgattaaa
    54181 tcttgtgcgc cagctaaatc tgcgagtctt cactctgact gataaagctg gctgcaagaa
    54241 aatgacacag agagccgaat tatctcccgt tcggctctct ctccttttac tcattattca
    54301 gatcagaatt gccacttaat gccaaccatt gcagaggtgt cattatatcc cctatcggca
    54361 acctgaacgc ccacattccc ccagacattc aggttggcat ttagctgtcc ttccaccccg
    54421 gtttttatct cagcaatatt tcgggctcca tcctgagtga cggacacgcc atccatactg
    54481 gtgctgaaat ccttactgtt atgcagccag ttcacttcta caaacggctg gaactcgcgg
    54541 gatttaccgt catccatttt atggtggctc ttcagccagg tttttacgcc aagtcgggtt
    54601 tgaacattgc catcaccgtt gctatgaacg agggttccgt tgctttcgcg gtgcttatcg
    54661 gctttgactc ccatccaggt aacctgtgct tgcggctgaa cataccattc attacgcgtt
    54721 ccctggctgc cattaaattc agctaatttg tgtttgtatc cagcttccag tgaagcggta
    54781 aatccttttg atttatagga ttcactttgt aagtcatccc ctttcactgt gttatcaaac
    54841 cagctgtact gcgcccaact gtcgagatac gcgccattac gcgactcgtc atcggcatac
    54901 caggtggcat agaggccggt gctatatccg ttcacactcg cttttgcacg ataaccggtt
    54961 cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg ccatgacccc aacatgccag
    55021 ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc ccagttgcag aacatagcga
    55081 ttgctttggg ttttcagctg gccgctgcca tcacgccatt tattatgacc accttcatgg
    55141 cgcatccaca tagtggtttg tttctgctca cccgtcacca tgtcggtata gtacgtatta
    55201 cccagacgct catgcagacg cgtggtgaac attgtattcg ctgctgcaag gttcgcaatg
    55261 tagctacccg cctccggtcg caggtcatta tccacattca gatccggacc cggcgtaggt
    55321 gtcgggttag ggttcggctc tggattcggg tttggctccg gattcggcat cgggtctggc
    55381 tccggctgca gttcaggaga atcactgccg ctggtcagat accagttacc actatttgcc
    55441 ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa cgatacgacc agcctgaaca
    55501 aattcgccct cagacttacc gtctacatgg ataagttcga taccgttaag ggttttcgca
    55561 cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc cgctagtatc gccctcgaca
    55621 accaatttat cggttactga gtcatcgcca ttcagtaccg tattcattac tagttgaccg
    55681 ttattaccgt ggtagttccc cttcaccact aacacattgc cagcttgctg tcctgacatt
    55741 ccggtcatca gcgtaccgtt gttggtcaga ttgccgccca gggtgtaatt cacagacgca
    55801 gccgctcttt tttcagtgct attattcaac gccagggtac cgttgttctc aacgtttgcg
    55861 ctacccaatc cctgcgcatg acttaccagg acattagccg aattagcaat gttcacattc
    55921 gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc cgccctgaat attcagctct
    55981 ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg taaccgtcag actgccgcca
    56041 tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc ccacggtctg gctgtgacca
    56101 ttcatatcca gttcagtctc tgccgccaga cgcagttcat gggtattacc caacacgtta
    56161 tcgttattca acaaaagcgt cccactgcgc agatcggtaa caccggtata gtcgttgtct
    56221 ttgttagaaa gcgatacggt ctgacccttc tggctgctga atgccaggtc accactgccg
    56281 gtaatctttg cgctgagatc ggcggcattc tcgcttttac cgttagagct cagcaccaga
    56341 gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc cgtaaccgat atacagaccg
    56401 tcattgttta ccccactcgt cagacgatag tcgtaagtgc cgacggccgc tttttgccca
    56461 ttctgctgaa tatcacgttg ggcgctgctg gagaggattt gcccattttt atcctgcaat
    56521 tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg catcaaccag cttaatggcg
    56581 ctgttaccat catcgacttc agtgagcgat aacgcagagt caatatcacg agatactgag
    56641 cggacaacgt cactgtcaga aacctgaatc acaccctcgc cgcgcaaatc cagcgtttta
    56701 ctaacgttga ccgtcccttc tgtcatctgt gcacctgctg tcagggcacc aaaatcaacg
    56761 gttccgccgg agaatcccaa agtgctaatg gagtgagcgc ccttacccac tgttacctgg
    56821 cttccctgac ccgcagtgat cccgcctctc tgcagaaact cagcattctt ttccaggtta
    56881 aatgtcgtgt tctgaaacgc cagtgaaccc tggaaccagt ctgtataagc aaactcatcg
    56941 aaggagaatt cgtgattccc catgtttatt tcaacaatac ctctaccttg aagatcgtta
    57001 tcaaataccc aatctttatt ggcattgatt tgcagtttgc catcaacatc caggtccgca
    57061 cggccgatgt tcttgtcttc agaaataacc gcgacagcac cagaagcgac atcaatatat
    57121 ccgctgaatg catagttatt gccggtcagt gccactgtcc cactgtcgat acttattgta
    57181 ccattgccgg aaatcttatt ggcaaaggtc atgctatcgt cagccttcag gtgtaatttc
    57241 ccgcggttgt tgatctctgc tgtacctata tttttggtga tccctaaagc gccattttgc
    57301 tcaattttga ctttaccact aaaggcagag ttatcaccgg ttaagtaagt cgtgccattg
    57361 agcgcattga tatagcccag gccctcgctg ccactgacaa tatccgcgga aatagtcgcg
    57421 tttttatccg tgtggtttaa ggtgattttc ttatcggatg tctttgccca cacaaactca
    57481 attttttcgg catcaataat ccctgcagcc ttcgcagcac ttccttcctg tgcacctaac
    57541 gctaactcag agttggtgct taaactaatt ttcggtgcgg atattttacc tccatctgat
    57601 acgactaatg ttgtatcaga acgttggtcg taataaccca ggctaatatg ttcacgagca
    57661 ttaacttcac caccgttatt aaccgacagg aactgattac cgccatatcc gccgtaaacg
    57721 cccataaaga aggtccctgc atttacctga ctaccttcgc cgcttacatt cccctgcgta
    57781 taagtcagac gactggtgcc cttaacatga attttgccgc cattggagac attaaaatag
    57841 ccttgatcat gtctcatgcc tgagtttgac tcatacaaat catcagcata taactcgctg
    57901 ccagcattgg agacattcag gaaccccgta cctttcagca aaaagttaag cgggccattg
    57961 attgttacct ttcctccggt aatatccaac ccaccaccag aagcgagagt cagacgcgga
    58021 cgggaagtat ctgtcgtatt gattaccaga ttggcattgt cagtcaccgt aatacggtaa
    58081 tcgatgctct ggttctcttt gccaaaatca atagttttat cctggtttac gatgacatca
    58141 tcggcgaata ccagagatga atataaactt atcgcgctaa ttaaaacagc ccgggacttt
    58201 tttcctttta ctctgctccc cagctcagag cagacgctcc aacagttacg ggacctgttc
    58261 cactttagcc gatatatttt attcatcaca atctccagca atacatccaa ataaaaccaa
    58321 gattgcataa acagcaacgc attaaatgcc attctggtta ctgttcatcg catataaaaa
    58381 ttcaaaaatt ttgcatagca ttggcaacca catatctgag tatgtagttc agagggtaat
    58441 aaaatagcaa gttattactt aaaacttatt ttaagttaac actcttaact ttatcattac
    58501 attcctccca gtct
//
LOCUS       DAZFFI010000016        58504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFFI010000016 DAZFFI010000000
VERSION     DAZFFI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.016"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..171
                     /gene="yhfL"
                     /locus_tag="ACHJ12_001235"
     CDS             4..171
                     /gene="yhfL"
                     /locus_tag="ACHJ12_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4989057.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            467..1804
                     /gene="frlA"
                     /locus_tag="ACHJ12_001236"
     CDS             467..1804
                     /gene="frlA"
                     /locus_tag="ACHJ12_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4989058.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1825..2847
                     /gene="frlB"
                     /locus_tag="ACHJ12_001237"
     CDS             1825..2847
                     /gene="frlB"
                     /locus_tag="ACHJ12_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4989059.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTREEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2897..3727
                     /gene="frlC"
                     /locus_tag="ACHJ12_001238"
     CDS             2897..3727
                     /gene="frlC"
                     /locus_tag="ACHJ12_001238"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO4989060.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3724..4509
                     /gene="frlD"
                     /locus_tag="ACHJ12_001239"
     CDS             3724..4509
                     /gene="frlD"
                     /locus_tag="ACHJ12_001239"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO4989061.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4609..5340
                     /gene="frlR"
                     /locus_tag="ACHJ12_001240"
                     /gene_synonym="yhfR"
     CDS             4609..5340
                     /gene="frlR"
                     /locus_tag="ACHJ12_001240"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4989062.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5492..6577)
                     /gene="yhfS"
                     /locus_tag="ACHJ12_001241"
     CDS             complement(5492..6577)
                     /gene="yhfS"
                     /locus_tag="ACHJ12_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4989063.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6589..7893)
                     /gene="yhfT"
                     /locus_tag="ACHJ12_001242"
     CDS             complement(6589..7893)
                     /gene="yhfT"
                     /locus_tag="ACHJ12_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO4989064.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7905..8258)
                     /gene="yhfU"
                     /locus_tag="ACHJ12_001243"
     CDS             complement(7905..8258)
                     /gene="yhfU"
                     /locus_tag="ACHJ12_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO4989065.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8269..9147)
                     /gene="php"
                     /locus_tag="ACHJ12_001244"
     CDS             complement(8269..9147)
                     /gene="php"
                     /locus_tag="ACHJ12_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO4989066.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9144..10370)
                     /gene="yhfW"
                     /locus_tag="ACHJ12_001245"
     CDS             complement(9144..10370)
                     /gene="yhfW"
                     /locus_tag="ACHJ12_001245"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4989067.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLI
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10370..11533)
                     /gene="yhfX"
                     /locus_tag="ACHJ12_001246"
     CDS             complement(10370..11533)
                     /gene="yhfX"
                     /locus_tag="ACHJ12_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO4989068.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHFFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(11617..11979)
                     /gene="yhfY"
                     /locus_tag="ACHJ12_001247"
     CDS             complement(11617..11979)
                     /gene="yhfY"
                     /locus_tag="ACHJ12_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO4989069.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11996..12901)
                     /gene="yhfZ"
                     /locus_tag="ACHJ12_001248"
     CDS             complement(11996..12901)
                     /gene="yhfZ"
                     /locus_tag="ACHJ12_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO4989070.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13170..14174)
                     /gene="trpS"
                     /locus_tag="ACHJ12_001249"
     CDS             complement(13170..14174)
                     /gene="trpS"
                     /locus_tag="ACHJ12_001249"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4989071.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14167..14925)
                     /gene="gph"
                     /locus_tag="ACHJ12_001250"
     CDS             complement(14167..14925)
                     /gene="gph"
                     /locus_tag="ACHJ12_001250"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4989072.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14918..15595)
                     /gene="rpe"
                     /locus_tag="ACHJ12_001251"
     CDS             complement(14918..15595)
                     /gene="rpe"
                     /locus_tag="ACHJ12_001251"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4989073.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15613..16449)
                     /gene="dam"
                     /locus_tag="ACHJ12_001252"
     CDS             complement(15613..16449)
                     /gene="dam"
                     /locus_tag="ACHJ12_001252"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4989074.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16556..17842)
                     /gene="damX"
                     /locus_tag="ACHJ12_001253"
     CDS             complement(16556..17842)
                     /gene="damX"
                     /locus_tag="ACHJ12_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4989075.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEAEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17934..19022)
                     /gene="aroB"
                     /locus_tag="ACHJ12_001254"
     CDS             complement(17934..19022)
                     /gene="aroB"
                     /locus_tag="ACHJ12_001254"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4989076.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19079..19600)
                     /gene="aroK"
                     /locus_tag="ACHJ12_001255"
     CDS             complement(19079..19600)
                     /gene="aroK"
                     /locus_tag="ACHJ12_001255"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4989077.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20001..21239)
                     /gene="hofQ"
                     /locus_tag="ACHJ12_001256"
     CDS             complement(20001..21239)
                     /gene="hofQ"
                     /locus_tag="ACHJ12_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4989078.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21151..21555)
                     /gene="hofP"
                     /locus_tag="ACHJ12_001257"
     CDS             complement(21151..21555)
                     /gene="hofP"
                     /locus_tag="ACHJ12_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4989079.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21545..21985)
                     /gene="hofO"
                     /locus_tag="ACHJ12_001258"
     CDS             complement(21545..21985)
                     /gene="hofO"
                     /locus_tag="ACHJ12_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4989080.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21969..22508)
                     /gene="hofN"
                     /locus_tag="ACHJ12_001259"
     CDS             complement(21969..22508)
                     /gene="hofN"
                     /locus_tag="ACHJ12_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4989081.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22508..23287)
                     /gene="hofM"
                     /locus_tag="ACHJ12_001260"
     CDS             complement(22508..23287)
                     /gene="hofM"
                     /locus_tag="ACHJ12_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4989082.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            23407..25959
                     /gene="mrcA"
                     /locus_tag="ACHJ12_001261"
     CDS             23407..25959
                     /gene="mrcA"
                     /locus_tag="ACHJ12_001261"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4989083.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGIDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26124..26684)
                     /gene="nudE"
                     /locus_tag="ACHJ12_001262"
     CDS             complement(26124..26684)
                     /gene="nudE"
                     /locus_tag="ACHJ12_001262"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4989084.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27004..29139
                     /gene="igaA"
                     /locus_tag="ACHJ12_001263"
     CDS             27004..29139
                     /gene="igaA"
                     /locus_tag="ACHJ12_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4989085.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29204..29872
                     /gene="yrfG"
                     /locus_tag="ACHJ12_001264"
     CDS             29204..29872
                     /gene="yrfG"
                     /locus_tag="ACHJ12_001264"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4989086.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29883..30284
                     /gene="hslR"
                     /locus_tag="ACHJ12_001265"
     CDS             29883..30284
                     /gene="hslR"
                     /locus_tag="ACHJ12_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4989087.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30309..31187
                     /gene="hslO"
                     /locus_tag="ACHJ12_001266"
     CDS             30309..31187
                     /gene="hslO"
                     /locus_tag="ACHJ12_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4989088.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31322..33046)
                     /gene="yhgE"
                     /locus_tag="ACHJ12_001267"
     CDS             complement(31322..33046)
                     /gene="yhgE"
                     /locus_tag="ACHJ12_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4989089.1"
                     /translation="MDNVELSPATRWGMIATGLLQDLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNHWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPLLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33425..35047
                     /gene="pckA"
                     /locus_tag="ACHJ12_001268"
     CDS             33425..35047
                     /gene="pckA"
                     /locus_tag="ACHJ12_001268"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4989090.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35123..36475)
                     /gene="envZ"
                     /locus_tag="ACHJ12_001269"
     CDS             complement(35123..36475)
                     /gene="envZ"
                     /locus_tag="ACHJ12_001269"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4989091.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36472..37191)
                     /gene="ompR"
                     /locus_tag="ACHJ12_001270"
     CDS             complement(36472..37191)
                     /gene="ompR"
                     /locus_tag="ACHJ12_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4989092.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37419..37895
                     /gene="greB"
                     /locus_tag="ACHJ12_001271"
     CDS             37419..37895
                     /gene="greB"
                     /locus_tag="ACHJ12_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4989093.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37992..40313
                     /gene="yhgF"
                     /locus_tag="ACHJ12_001272"
     CDS             37992..40313
                     /gene="yhgF"
                     /locus_tag="ACHJ12_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4989094.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40751..40978
                     /gene="feoA"
                     /locus_tag="ACHJ12_001273"
     CDS             40751..40978
                     /gene="feoA"
                     /locus_tag="ACHJ12_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4989095.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40995..43316
                     /gene="feoB"
                     /locus_tag="ACHJ12_001274"
     CDS             40995..43316
                     /gene="feoB"
                     /locus_tag="ACHJ12_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4989096.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43316..43552
                     /gene="feoC"
                     /locus_tag="ACHJ12_001275"
     CDS             43316..43552
                     /gene="feoC"
                     /locus_tag="ACHJ12_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4989097.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43755..44633
                     /gene="rpnA"
                     /locus_tag="ACHJ12_001276"
     CDS             43755..44633
                     /gene="rpnA"
                     /locus_tag="ACHJ12_001276"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4989098.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLSWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44662..45432)
                     /gene="bioH"
                     /locus_tag="ACHJ12_001277"
     CDS             complement(44662..45432)
                     /gene="bioH"
                     /locus_tag="ACHJ12_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4989099.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45470..46153
                     /gene="gntX"
                     /locus_tag="ACHJ12_001278"
     CDS             45470..46153
                     /gene="gntX"
                     /locus_tag="ACHJ12_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4989100.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46212..46787
                     /gene="nfuA"
                     /locus_tag="ACHJ12_001279"
     CDS             46212..46787
                     /gene="nfuA"
                     /locus_tag="ACHJ12_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4989101.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47148..48464
                     /gene="gntT"
                     /locus_tag="ACHJ12_001280"
     CDS             47148..48464
                     /gene="gntT"
                     /locus_tag="ACHJ12_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4989102.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48509..50593)
                     /gene="malQ"
                     /locus_tag="ACHJ12_001281"
     CDS             complement(48509..50593)
                     /gene="malQ"
                     /locus_tag="ACHJ12_001281"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4989103.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPVNPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50603..52996)
                     /gene="malP"
                     /locus_tag="ACHJ12_001282"
     CDS             complement(50603..52996)
                     /gene="malP"
                     /locus_tag="ACHJ12_001282"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4989104.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQHFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53608..56313
                     /gene="malT"
                     /locus_tag="ACHJ12_001283"
     CDS             53608..56313
                     /gene="malT"
                     /locus_tag="ACHJ12_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4989105.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56356..57372)
                     /gene="rtcA"
                     /locus_tag="ACHJ12_001284"
     CDS             complement(56356..57372)
                     /gene="rtcA"
                     /locus_tag="ACHJ12_001284"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO4989106.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMCGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57376..58478)
                     /locus_tag="ACHJ12_001285"
                     /pseudo
     CDS             complement(57376..58478)
                     /locus_tag="ACHJ12_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
BASE COUNT        13863 a        16127 c        14779 g        13735 t
ORIGIN      
        1 taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca
       61 tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg
      121 gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac
      181 agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt
      241 tgtttctata tccttcttgc tccccacccg taaaaatatt ttcactttaa tttcaattgg
      301 ataaccacat tcattagcca cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca
      361 ttacataaca tcatatgttg ttatattcat catgtattgt catgttacct tttaaatgac
      421 tgcaaactct cccctacaac gtattggaca agaaaaaggt atcgctatgg gaagccagga
      481 actccaacgc aagctcggat tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg
      541 ctccggtatt tttgtatctg tgggtgaagt ggcaaaagca gcgggcacgc cgtggcttac
      601 ggtgctcgcg tttgtcattg gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga
      661 actatccacc gcttatccgg aaaatggcgc agattatgtt tatctgaaaa atgccggaag
      721 ccgaccgctg gctttcctct ccggctgggc cagcttctgg gccaacgatg cgccgtcatt
      781 gtcgattatg gcgctggcga ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt
      841 gctcggtaaa tttatcgccg ccggattaat tatcgccttt atgttgctac acctgcgctc
      901 cgttgaaggc ggcgcagcgt ttcagacgct aattaccatc gccaaaatta tcccgttcac
      961 tatcgtcatt ggccttggga tcttctggtt taaagcggag aattttgccg cccctaccac
     1021 cactgcgatt ggcgcaacgg gcagctttat ggcgctgctg gcggggatct ctgccaccag
     1081 ttggtcgtat accggcatgg cctctatctg ttatatgacc ggcgaaatta aaaaccccgg
     1141 aaaaaccatg ccacgagcgc tgattggttc ctgtctgctg gttctggtgc tctacaccct
     1201 gctggcgctg gtgatttccg gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc
     1261 gatttccgac gccctgacct ggatccccgc actcggcagc accgctggga tctttgttgc
     1321 catcacggcg atgatcgtca ttcttggttc gctttccagc tgcgtgatgt accagccgcg
     1381 gctggaatac gcgatggcga aagacaacct gttctttaaa tgcttcggcc atgtgcatcc
     1441 gaaatacaac acgccggatg tctccatcat cctgcaaggg gcgctgggga tcttcttcat
     1501 cttcgtttcc gatctcacca gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa
     1561 taccctcacc ttcggctcca tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg
     1621 gcgtactccg gctttcgggc tgatgaccac cctcgccatt gcgtcaagcc tcattctggt
     1681 cgcctcaacc tttgtctggg caccgattcc cggccttatc tgcgccgtca tcgttattgc
     1741 tactggtctg cctgcttacg ccttctgggc gaagcgtagc cgccagctca acgctttgtc
     1801 gtaatgttat ctggagaaaa taaaatgttg gatattgata aaagcaccgt ggactttctg
     1861 gtcaccgaaa atatggttca ggaagtggaa aaagttctca gccatgacgt tccgctggtg
     1921 cacgccatcg tggaagagat ggtgaagcgc gacattgatc gtatttattt cgttgcctgc
     1981 ggctcgccac tcaacgcggc gcaaacggcg aaacatctgg cggatcgctt ttccgatctt
     2041 caggtctacg ccatttccgg ctgggagttc tgcgataaca ccccgtatcg cctcgacgat
     2101 cgttgcgcgg tgattggcgt ttctgactac ggtaaaacgg aagaggtaat caaagcgctg
     2161 gagctgggcc gggcctgcgg cgcactcact gcggcgttca ccaaacgcgc ggacagcccg
     2221 attacctcgg cggcggaatt tagcattgat tatcaggccg actgtatctg ggaaattcac
     2281 ctgctgctct gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa
     2341 atcggcaaga tcaaaaacga tctcaagcag ttgccgaatg cgctcggtca tctggtacgc
     2401 accagggaag aaaaaggccg ccagcttggt gaactggcca gccagtggcc gatgatttat
     2461 accgttgctg cgggtccgct gcgtccgctg ggttacaaag aaggcattgt aacgctgatg
     2521 gaatttacct ggacgcacgg ctgcgtgatt gagagcggag agttccgcca tggcccgctg
     2581 gagattgtcg aaccgggcgt tccgttcctg ttcctgctcg gcaatgatga aagtcgccac
     2641 accaccgaac gcgccattaa ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat
     2701 tacgccgaaa tttcgcaagg gctgcacccg tggctggcac cgttcctgat gttcgtgcca
     2761 atggagtggc tctgctacta cctgtctatt tacaaagatc acaacccgga tgaacgccgc
     2821 tattacggtg gtctggtgga atattaatcc ctctcccggc ccggcaacgg gccggattta
     2881 cgcaaggagt tacccgatga aaacaggtat gtttacctgc ggccaccagc ggctgcctat
     2941 tgaacacgca tttcgtgatg caagcgagct gggttacgac ggcatcgaaa tttggggcgg
     3001 tcgcccgcac gcgttcgcgc cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct
     3061 ggcgcagacg tatcagatgc cgattatcgg ctatacgcca gaaaccaacg gctatccgta
     3121 taacatgatg ctgggcgatg aacatatgcg tcgcgaaagc ctcgacatga tcaagctggc
     3181 gatggatatg gcaaaagaga tgaacgcggg ttatacgctg atttccgcgg cccacgcggg
     3241 ctatctcacg ccacctaatg ttatctgggg acggctggca gagaacctaa gcgaactgtg
     3301 tgagtacgcg gaaaacatcg gcatggatct aatcctcgaa ccgttaacgc cgtatgaatc
     3361 gaacgtcgtg tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg
     3421 cctgttcagc atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta
     3481 tttcgacaaa ctgggcgata aattacgtca tctgcatatt gtcgacagcg acggggccag
     3541 cgacacgcat tacattcctg gagaaggcaa aatgccgctg agggaactga tgcgcgatat
     3601 tatcgaccgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt atatgaacga
     3661 gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc cggaggatga
     3721 gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc gcaactgaat
     3781 aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg ctacggcata
     3841 cagccgggat gcattacctg ggtgggtgac gatgactacg gcacaaagct gaagcaggat
     3901 ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt taccgcacaa
     3961 actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga aggcgtgatg
     4021 gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga cattgtgcac
     4081 gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc gggcaaactt
     4141 accgctttcg acttctccga caagtgggac agctcgctct ggcagacact ggtgccgcat
     4201 ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct gaagatgaaa
     4261 gcgattgttg cccgtggcgc aggcgtggtg attgtcacgc tgggtgaaaa cggcagcatt
     4321 gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt tatcgacacc
     4381 atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc ggggatgaca
     4441 ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca gtaccacggt
     4501 gcctggtagg tataacgttg gcgtgagcat cttcacgcca acgtgctgtt acttgccgga
     4561 aaacgaccct ataatccgag taattcattc tttatttcag ggtcgattat gtcagctacg
     4621 gaccgctact ctcatcaact cctctacgct accgtccgcc agcgactgct ggatgatatc
     4681 gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct ttgtacacaa
     4741 tataacgtca gccgcattac cattcgcaaa gccatcagcg acttagtggc agacggcgta
     4801 ctgatccgct ggcagggaaa aggcaccttt gtacaaagcc agaaagttga aaacgccctg
     4861 cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac gaaagagaaa
     4921 gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa catccccgga
     4981 aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc gctgtttatt
     5041 gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta cgtcgaagga
     5101 agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag
     5161 accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg cgaactgggc
     5221 gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc
     5281 tccgaactct tctgccgcgc caatcgcatc accttaacta ttgataataa aagacattaa
     5341 ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg ttgaatttta
     5401 catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg accgttcagt
     5461 acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc tcacgcaaaa
     5521 tccgcagcac cgtctcttca ccgctgcgat tcgggttaat gcgaatcgca caatgttctg
     5581 attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc gggatttcat
     5641 atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc tcttccagca
     5701 ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc gcattagcaa
     5761 tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc aaccgttcag
     5821 atacccctgc ctgcaccgcg tgcatcactg gcgcaaacac cagaccacgc aatacttcca
     5881 gcgcctgtgc gccctggatc tggctaccgc cggagtaaag cgtggcgcga atacggttga
     5941 taacatcagc atcgccgacc actgcaccaa caccctctgg cccaaatagc ttgaagcagg
     6001 aaaatgtcga gacattcgcg ccgcattcac agccgattcg cgccaccttc atcaccgcat
     6061 agttgtcatc ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca
     6121 gcacgtagct gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca tccggttgtt
     6181 gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcaacagta ataagcgtca
     6241 gccccatctg ctcaataata acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa
     6301 gacgctgccc cggtttgagc aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc
     6361 cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt tccacacgct
     6421 gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca gtaagaaact
     6481 cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt tgctgcgcct
     6541 caataatcgt caggctttgc agaggaaacg tcttcatagg tgccttcctt aagccggaac
     6601 gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta ctgccacgac
     6661 cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt aaatggcaac
     6721 cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg aaccgaccag
     6781 cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg cattacgcac
     6841 cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt ccgccgaaat
     6901 aaccactgcg cctaataccg ctgcaaccat cggattcggc gagagatagc ccaccgcata
     6961 aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg tggcaatcaa
     7021 cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga tcagcgtttg
     7081 cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga tcgacacttc
     7141 actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca cgatggcgat
     7201 atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa gcccgctggc
     7261 atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat
     7321 caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat agcgcacgac
     7381 taccacacgg gtcatcagta ccaccacggc ggcgatcaga ctttgcttcc agccaaactg
     7441 gtagaaaatc gccaccagcg ggaacagtgc aaaagctgaa accaccggcg agcttaattc
     7501 ccccaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta ctggcaacag
     7561 gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca ggctgtttat
     7621 cgccaggacg ccgaggatat cggtaggaag aaagagtaac catgcgttga gcagcccggt
     7681 tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac caaaagcgat
     7741 actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga tcgggcggat
     7801 gccgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca
     7861 cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt cagatggtta
     7921 atcaacatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc cacttttcct
     7981 tcggcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt ggcaatggtg
     8041 caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta
     8101 ttacacgcgc cgatgtaata atccagttgc ccggatttca ctttcattgc cgcttccatg
     8161 tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt ttttttaatc
     8221 tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt attggaaaaa
     8281 ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc ctgactggcg
     8341 caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat tggcttttaa
     8401 atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc cacggtcacg
     8461 tagcgcatga agcatcgcaa tacgcttttc gtccgggtag taactgttct taccaatggt
     8521 gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga ggttgtcttt
     8581 cagatcgcag tgaccaacgg tgacgcgcga aagatcaacc ccgtgggctt gtagcaacgc
     8641 cagttgctcc agccccatcg tgctgaacga cgtatgcgtg gagatcgggc gtccggtctg
     8701 gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa tctttccttc
     8761 gctggtgccg atctccgcga tgatcccggc tttcagctcc gtgccatcga taccctgttc
     8821 aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcgcca catgttccgg
     8881 gaaaaacgcg tcctggtaat aaccggtaca ggccaccacg ttgatccccg tctcgcgcat
     8941 tacatcaagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct caatcacatt
     9001 acgcacgccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat actgatcaag
     9061 gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct catgggcgag
     9121 ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag ggaggaaaga
     9181 aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt agcccccaca
     9241 tcagaaagcg tggtgcgaac acctagctga gtagcagcca ggccttgctg ataaaccagc
     9301 actggcacca cttcacgggt atggtggctg tgaccaatgg tcggatcgtt accgtgatcc
     9361 gccatgacga tcaggcagtc atctggctgc atcgcctcaa caagccgggc aaggttacgg
     9421 tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc agcgaggtcg
     9481 gtttcctgga tattggtgca aataaacgcc gtcggatgcg tgttaaattc gtcgagggtg
     9541 atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata aggattgctg
     9601 acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata cagtttttgc
     9661 ggcacctgca ctttttcatc aacaccgtag cccatatgca cgacctggaa accgttgtca
     9721 taagcgccag aacgcggcgc attaatacca ataaagcgcc cttctttgct ttctgcggca
     9781 tcgagaatac gctgactgtt ggttaacagg ccgccaaatg taatgacccg accgacctgt
     9841 acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat cacagagaga
     9901 ttggcggtga tgttatagac ctggcctaaa tccgcctcga gattatcgcc aatcgcaacc
     9961 gcctgattga cccacagaaa ttgcagatca tcgccacggc gctccacctg ccaaccggcg
    10021 gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg cagcggcggt
    10081 aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc ttcatgttgc
    10141 agctctgcca cgccccaggt tgcggagtct gacggctgca tatcgcctgg cgcataaccc
    10201 aatgtgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg caactggctc
    10261 aggatgtgac cacaggtatt cgccccggca tcctgcgggc gcaccagcgt gacatctttc
    10321 attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat tacgccccca
    10381 gagagtttcc aagactgtca taacgaccga cgatttccgg ttcgccacga tgaataccag
    10441 acaccagcac cacatcactg cgggtgacaa aaatctgcgt gcgaaaacag agcactactg
    10501 cactgcttac cggaaactcg cctgccagcg gcagggtgta gtcgatactg ctgtcatcca
    10561 cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc agcgcatgtt
    10621 gcgcgtgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca cggaaaaaat
    10681 gggagatttc acttaaccag agcatcgcga tacgttcagg ctgatcgccc tgctggtttg
    10741 ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg tattgcgcca
    10801 gcaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca atcgcaatac
    10861 cagatttcgc cagttgatcc cgtgcttgta ccagcgtgtg aagattcggt gtcggcaaaa
    10921 ctttcccggc agcctcatcc caaagcaggc aagggaaatg ggttagtccc gccaaatgca
    10981 gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt acaaaaccgc
    11041 tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc acagactgga
    11101 ttcgcccggc cttcaccgcc gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg
    11161 tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg atttgtacca
    11221 gatgcccctg atgcgccacc ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat
    11281 ccaccgccac aatgccgctg tagcccaacg ccagcagttt ttccgccagc catgggttac
    11341 gaccgaattg tttggtcatc agatacagtt caatgccgta aagccgcgcc gtctcaatca
    11401 gccgcttacc gttttccagt atctgatcca cgtcgatcac ccagctgtcc ggggcgattt
    11461 tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg
    11521 cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc atttttttga
    11581 atatttaggt tgtgcgacgt ggatttgtca ggagcttcaa acctcttcgt tagcagccat
    11641 ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa cttccagtgc
    11701 gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc tggattgcgc
    11761 cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc gactgcgcat
    11821 cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca ccggcagatg
    11881 gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt ccttatcaat
    11941 aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct ttcgtttaat
    12001 aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg gcgtgtttat
    12061 ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg
    12121 cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct aatcccagca
    12181 tggtcagttc gttttccgcc accacgttcc agatcaccgc atcgacatcg cctttaacaa
    12241 tgcgttgtaa actctcgtga taagagagat cgactcgttc cacatcacta ccgccaaaaa
    12301 aaacatcggt catgattttc tgatccgccg aacggttatc cagccccacg cgcttcacgt
    12361 ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg tgcggcccca
    12421 actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc agtcgggaaa
    12481 ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc gcgccgcgca
    12541 tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc gctaaacctt
    12601 cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata tccacatgac
    12661 tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca cggcgttcaa
    12721 tccgtatcgc tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg
    12781 aacggcattc agttgccagc tcatctatgg ttttcaacct attaccgcat ttttcaccca
    12841 acaaataacg ggccagcgtc gtgataacga cgccttcttt tttgataaac gttcgacgca
    12901 taatatattt tcaataaaat gaatgtttat atcttcatta aaatgaagat aagtggcaac
    12961 tgtgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca aatgaggata
    13021 aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag
    13081 caaagggtaa ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg
    13141 ggcgagggga ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg
    13201 tacaccgctt tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc
    13261 acctgttgca ggaaggcttc atcgttgcgg aaacgatgat agcgttcctg caattcagtc
    13321 agcataccgg aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg
    13381 aactgttttt ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    13441 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca
    13501 gtgaccgcgc gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg
    13561 ttattgcggt tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg
    13621 cgcgcgccag atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg
    13681 ttgaaacgct gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc
    13741 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagtcaaac
    13801 agaccagcgt tgatgttctc ggcataacgc gcagatttat ctttaaactg ggtcatgcgg
    13861 ctcagttcgc cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt
    13921 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    13981 gccagcgtat ccagcgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    14041 tgttggtcaa cgatacagta aatgcaatgg tagtcatcct gcatgtttac ccactgacgc
    14101 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    14161 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    14221 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    14281 gttatatccg taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc
    14341 attgcgtgag tcgccgacaa acagcatctc ttgtggggca attcccatcc gctcagccac
    14401 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac
    14461 gctaaagtat ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    14521 gttggtgacc aggcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac
    14581 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcta tagtagcgat cgaacagttt
    14641 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttacccat
    14701 tgttttacgc agagtcgcac gttcctgacg cgcccaggcc aatgcacgct ccatcagaac
    14761 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gtaactccaa
    14821 cgcatacagc gccatatcta ccgcagcagc aagaccggga gcactgtcca ccagcgtacc
    14881 gtcgagatca aaagcgacgc cgcgaatatc ttcaaactta ctcatgactt acctttgcca
    14941 gttcactgcg catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac
    15001 cggcgacgaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    15061 caccgtccac ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc
    15121 gcagtttatc cagtgtttga ggaatgaaag actgaccgcc gaaaccagga ttgacggaca
    15181 tcagcaggat cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg
    15241 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    15301 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    15361 gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccat
    15421 agttacgcaa ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat
    15481 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    15541 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    15601 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    15661 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    15721 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    15781 cactggaata tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    15841 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata
    15901 aggcggatcg caatagacga cggatgcatc atctgcgcgc gccatgctat cggcgtaaga
    15961 ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    16021 gaaatagggt tttttgtagc ggccaaacgg cacattaaac tcaccgcgca gattgtaacg
    16081 acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    16141 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    16201 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    16261 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    16321 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    16381 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    16441 tttcttcatg ctgactaact aattacacct tctccggctg tggagaaagc tccgacagca
    16501 tccagcgctt caggcaactc ccgcaacgat aaaccattgc gggagataac cttgattact
    16561 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tggacatctg
    16621 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    16681 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    16741 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    16801 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    16861 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    16921 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg
    16981 gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg
    17041 gttttttcgg ttcaatcact gcctgctggc gagctggacg cgtagtggcc ggacgttcag
    17101 cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt gcgaacaggc gcgacagtcg
    17161 cgggttcggt cggcaatgtg gaattgaccg ccacattgtt caactgttgc tgattttgtg
    17221 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    17281 ccgccggggt ttgcccttga gtcggcgtag aagagatcgg tggcagagaa acatcctgct
    17341 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    17401 cattgcctgc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagaggtcg
    17461 agggggcttt tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga
    17521 cacccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg
    17581 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttctgctt
    17641 cgatttccgg ttcctcatta cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    17701 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    17761 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    17821 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    17881 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    17941 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    18001 ctcttaccaa ttgccaacgg aagaattaag cgtatctctc ccgcaaggac tttcttgtca
    18061 cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac
    18121 ccagcccgct tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    18181 cgttccgacg tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa
    18241 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    18301 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    18361 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    18421 tccagccagt taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    18481 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    18541 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    18601 ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca
    18661 ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc
    18721 gccaccagcg tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg
    18781 agtacagcca ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    18841 cccgcctgtt caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgttg
    18901 gtgaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    18961 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    19021 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    19081 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    19141 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    19201 ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    19261 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    19321 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    19381 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    19441 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    19501 cgtaaaattc catattgagt tgttgagcta actggcgccc aatagtgctt tttccggcac
    19561 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    19621 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    19681 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    19741 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    19801 ccgctctggc gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    19861 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    19921 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac
    19981 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    20041 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    20101 tcgccaagca acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    20161 agcgccaacg tttctccgct tttgacctcg acctgcgttt cgatctcctg cttatcaatc
    20221 gccagcactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    20281 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg
    20341 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt
    20401 tcgctcccct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg
    20461 ataatatcca gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg
    20521 ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta
    20581 ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc
    20641 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    20701 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg
    20761 ttatctcgta gcaaaagacg attggtgcgt ttatcgacgg tcatactccc tttggcactc
    20821 agtagcttct cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg
    20881 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata
    20941 ttgttattct gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc
    21001 agtccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt
    21061 aacgacaccg taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc
    21121 agcgcctgca acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc
    21181 tttgccgcct gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt
    21241 tgtatctcct tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggttcccag
    21301 cgttagtgcg tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg
    21361 ctgcacccgt cgccatttct tttgcccgtc ttttattaca ccgatgatgt gctcgcctcg
    21421 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    21481 cggtttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    21541 gcgtttaacc ctcatgcggt gtctccagtt gtagcgtgaa caaaagatca tcaccttcca
    21601 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    21661 gcactgcttc ccacagcgtt ttcaacgcca actcacctcc ctgcgcggat ggatgccagg
    21721 aaaccagttg cgcgccggat aactgaaaat ccagaggcga aaagggcagc gttttttcct
    21781 cgctgaaggg agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg
    21841 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg
    21901 taactaacat cagcaaccag aatgcccagc aaagctggcg gaggcggggt gatgtggcga
    21961 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    22021 cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc
    22081 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    22141 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    22201 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc
    22261 gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg
    22321 cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct
    22381 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    22441 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    22501 gggttcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccacctg
    22561 acggcggtag cggcggctga cgaacagaaa cggcctccca gggatcaaat cccccttcgc
    22621 cacatatcgc gacgctttcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    22681 tcatccccac cgccagtttg cgcccccagc gatagcgtgt cgcccacagc cactgttcgt
    22741 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    22801 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agagtaagca
    22861 gcgttgccag ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt
    22921 cgctataatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg
    22981 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct
    23041 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    23101 tccaaggtaa caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa
    23161 tgtcgttctc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgta
    23221 cgatcgcaac cgctaccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    23281 atgccattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    23341 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    23401 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tttgctggga
    23461 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtggcgaca
    23521 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    23581 gctcaatacg gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg
    23641 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg
    23701 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg
    23761 agtaccatta cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgctgatg
    23821 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgag
    23881 atcctcgagc tttatctgaa caagatttac ctcggttacc gcgcctatgg tgtcggtgct
    23941 gcggcacaag tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggctgtg
    24001 atcgccgggc tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc
    24061 gtcgcgcggc gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccaacaa
    24121 cagttcgatc agacacgcac tgaggcgatt aacgctaact atcacgcgcc ggagattgct
    24181 ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    24241 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    24301 gcacagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    24361 ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat
    24421 acgctgaagg cgctgccaac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    24481 cctcagcaag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    24541 gttcgctggg cgcgtcctta ccgttcggat actcagcaag gaccgacgcc gcgtaaagtg
    24601 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    24661 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaacggtgcc
    24721 gttatggcgc tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    24781 gcactgcgtc aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    24841 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    24901 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt
    24961 caggggctgg gtcagtcgaa aaacgtggtg atggtacgcg caatgcgggc gatgggcatc
    25021 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    25081 gaatcgctgg cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg
    25141 gtcatggcga acggtggctt cctggtggac ccgtggttta tcagcaaaat tgaaaacgat
    25201 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattccg
    25261 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    25321 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    25381 caggcgttag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    25441 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag
    25501 ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    25561 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    25621 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    25681 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    25741 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt
    25801 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    25861 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    25921 accattatcg ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    25981 ttttcagttg gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg
    26041 gctaacaaaa tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg
    26101 tgcatcaggc aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg
    26161 aggaacagcg cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc
    26221 atatgcgcca gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat
    26281 tccgggtaga gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc
    26341 atgctgagct tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc
    26401 tcgcggttag cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct
    26461 aattcgtagg attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcgtccaca
    26521 atcggcacaa tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc
    26581 acgccattgc tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca
    26641 gtttcaacat tcagaatggt gggtttttgt aatgatttgc tcatcgtggg atctatgctg
    26701 tgaaatcagc ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta
    26761 tttacattta tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt
    26821 gaaataactc caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt
    26881 tactgtgatg tgcttaatga tgctcaagtt aaactccacg cttgccgata gccaaccgca
    26941 gaatcatgta ttgtgtctgg tgcgactgac cacgcctgac agactaagta agatggggaa
    27001 agcatgagca ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga
    27061 tggctgataa aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat
    27121 gcgcaaacgc gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc
    27181 ctgacgcagg tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg
    27241 gcgctgaatg ccgaaagcaa caacgtcatg atgctgacac acgctatcac gcgttacggc
    27301 atctctaccg acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg
    27361 ccccctttct gggaacaata catcaacgat gagaataccg ttgaactgat ttataccgat
    27421 tcgctgccgc tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg
    27481 cgcggctatg ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt
    27541 gagcaaatcg agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt
    27601 ccgcgcgggc tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc
    27661 ctgattaccc cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc
    27721 gcaggtctgt gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt
    27781 ctgcgcggta caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac
    27841 aatatttcac tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct
    27901 caggatctcg gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag
    27961 gggcgatatc tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc
    28021 agtacgatta tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg
    28081 ctggatatgc cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc
    28141 accagcgtaa aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc
    28201 ggtacgggaa tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct
    28261 tttttaccgt ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca
    28321 gaatctgaac tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac
    28381 cctaaaccgg aagatgaatc tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc
    28441 ggcatggtat tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct
    28501 gccaaagatg actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac
    28561 tgggacgcgc tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta
    28621 cgcccggtga gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc
    28681 acgcatgaaa cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg
    28741 attgtcagcg acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac
    28801 gactacccgc cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat
    28861 acgccgttta acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag
    28921 catattggcc ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca
    28981 ttgctgctac tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt
    29041 taccagcgtc atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac
    29101 ccgcaactga tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat
    29161 gctaccctgt cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc
    29221 ctggcaggac gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt
    29281 cgataactat ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac
    29341 gccacaggaa gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa
    29401 ctggtactgt cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac
    29461 cgagatggga ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa
    29521 agccagcggt aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa
    29581 acttgagcat acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg
    29641 ttatccgaaa gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc
    29701 tgaaagaacg ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg
    29761 tattcgttac tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca
    29821 acgccatccg tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg
    29881 ccatgaaaga aaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt
    29941 tttataaaac ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg
    30001 ggcagcgcag caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg
    30061 gaaatgacga acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg
    30121 aggcagcctt gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcgc
    30181 tggcacgtaa acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag
    30241 agcgccgcga cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag
    30301 agatgattat gccgcaacat gaccaattac atcgctatct gtttgaaaac tttgccgtgc
    30361 gcggcgaact ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaat cacgattatc
    30421 cgcagcccgt taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta
    30481 cgctgaagtt tgatggtgat atcaccgtac agctgcaagg cgacggtccg atgaatctgg
    30541 cggttattaa cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa
    30601 ttccagaaaa tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta
    30661 ccccgagcga aggcgaacgc tatcagggcg tggttggtct ggaaggtgat accctggcgg
    30721 cctgcctgga agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca
    30781 ccggcgacgt agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc
    30841 aaaatgccca gcaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg
    30901 aagaactgct gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg
    30961 tgacggttta cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg
    31021 ccgacgcgct gaaaacgctg cctgatgaag aagtcgatag catcctggcg gaagatggcg
    31081 aaattgacat gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg
    31141 ctgaaatccg caacaacgcg tctccggcag atccgcaagt tcattaatga gtatgtccgg
    31201 cagagagggc tctgccggat tttacgacgt aatggcctga tgtgctgcgc ttaccaggcc
    31261 tgtgaaataa atcgcaaatt attgttggtc agatacagcg tcgtatccgt taaaagagtt
    31321 gtcacttact taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt
    31381 tcggcctcgt ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt
    31441 cgttcggaag taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat
    31501 agacaatgac tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat
    31561 tcagatcttg ctcgaccagg acgcaggcgt ctttttcaat acaggtcatc acgttatagc
    31621 gatcctggat taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta
    31681 acacattatc ggctaatact ttttccgata cctgttcctg aagatgttgc tctccatcta
    31741 acgccatcag cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca
    31801 gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatatag agacttacct
    31861 ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca
    31921 tactgtccag cagcggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg
    31981 caaggttcac tttgccctga agaacaaggg gattttgccc attacgccag acgatactga
    32041 caaaataccc cagcgaccac accagtaaca ccagcaccgc cagcgcgccc tgcaagcggt
    32101 cgacagtcca gccgtactga gcgacccgca gccataacgc ccaggcggcg acgaacacat
    32161 acagcggagc aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa
    32221 gtgacgcttt ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg
    32281 ttaacagcaa cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca
    32341 gggtgatgat aaacatcagg gttagcaatg atactaacgg cagcaacccc gtggcgatta
    32401 gcgtgaacaa cttttgaata gagtcgatta aacgtgactg tgtacgcgcg agaatcaccg
    32461 ccagtgcggt aaccagacct aatgtaagat acataaacca gtcggttgca aaaaagagcg
    32521 tattgaaaaa cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc
    32581 acgtcaggcc attggcgaga aaaatcacca gtaatataag tacgttatgc catactgact
    32641 ggtaaaagta gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg
    32701 gtagcaacaa cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttctg
    32761 ctctccagtg gttcatgcca tcaatttgcc actttagcca accgctcatc cctagcgtgg
    32821 caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaggag atcacggaga
    32881 agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgagat
    32941 tttttccggc aagccaggcg atcagcaggt agcaaaccag atcctgaagt aatccggtag
    33001 caatcatccc ccagcgtgtt gcgggtgaaa gttcaacgtt atccatgtta gttatccaga
    33061 atcaaaaagt gggttaatta tcgcatccgg gccgtagtat tttgcttttt tcagaaaata
    33121 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccgat tgaatttgca tcaatttcat
    33181 tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt aaccttttaa
    33241 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    33301 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    33361 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    33421 tgaaatgcgc gttaacaatg gtttgacccc gcaagaactc gaggcttatg gtatcagtga
    33481 cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc
    33541 gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    33601 cggtatcttt accggccgct caccaaaaga taagtatatc gtccgtgacg ataccacccg
    33661 cgacaccttc tggtgggctg ataaaggcaa aggtaagaac gacaacaaac cgctctctcc
    33721 ggaaacctgg cagcatctta aaggcctggt gaccaaacag ctttccggca aacgtctgtt
    33781 cgttgtcgac gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcattac
    33841 cgaagtggcc tggcaggcgc atttcgtcaa aaacatgttt atccgcccga gcgatgaaga
    33901 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcaaaatgca ctaacccgca
    33961 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    34021 gcagctgatt ggcggtacct ggtacggcgg cgaaatgaag aaagggatgt tctcaatgat
    34081 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    34141 gaaaggcgat gttgcggtgt tctttggcct ttccggcacc ggtaaaacca ccctttccac
    34201 cgacccaaaa cgccgcctga ttggcgatga cgagcacggc tgggacgatg acggcgtatt
    34261 taacttcgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga
    34321 aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    34381 cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccgatcta
    34441 tcacatcgaa aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt
    34501 cctgactgct gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac
    34561 ccagtatcac ttcctctctg gcttcactgc caaactggcc ggtactgagc gtggcatcac
    34621 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccaac
    34681 tcagtacgca gaagtgctgg taaaacgtat gcaagcggca ggtgcgcagg cgtatctggt
    34741 taacaccggc tggaacggca ctggaaaacg tatctcgatt aaagataccc gcgctattat
    34801 cgacgccatc ctcaacggtt cgctggataa cgcagaaact ttcactctgc cgatgtttaa
    34861 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac
    34921 ctacgcttcc ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga
    34981 caactttgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    35041 gctgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    35101 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    35161 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    35221 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    35281 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    35341 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc
    35401 tgactttgat ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc
    35461 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    35521 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagtactgca ttaagatccg
    35581 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    35641 cgttgcactc ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    35701 tcatctcagt cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg
    35761 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    35821 cacgggtaac ggaacgcacc tccgaagcgc catactcacg cagcggcggc ggaataatcc
    35881 ctttaccaac ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa
    35941 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    36001 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    36061 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    36121 ggccgcccag ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    36181 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    36241 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    36301 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    36361 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    36421 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    36481 agagccgtcc ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg
    36541 cgctggatct tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    36601 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    36661 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    36721 ctcatcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    36781 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
    36841 acgaatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    36901 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    36961 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    37021 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    37081 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    37141 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    37201 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    37261 agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    37321 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    37381 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    37441 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    37501 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    37561 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    37621 gcatggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtcttttttg
    37681 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    37741 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    37801 tgctgaaaaa agaagtcggc gatctggctg tggtgaatac tcctgccggg gaagcgagct
    37861 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    37921 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    37981 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    38041 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    38101 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    38161 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaagtcca
    38221 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    38281 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    38341 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    38401 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    38461 ccgcgctgga cggcgcgcgc tatatcctga tggaacgttt tgccgaagat gccgcgctgc
    38521 tggcgaaagt gcgtgattat ctgtggaaga acgcgcatct ggtctcaacc gtggtgagcg
    38581 gtaaagaaga ggaaggggca aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    38641 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    38701 tttcgctgaa tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa
    38761 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag
    38821 gcgtagtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    38881 gtaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    38941 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    39001 gtactggggt aaaagtggcg gtggtcgatg ccactggcaa actggtagcg accgatacca
    39061 tttacccgca caccggacaa gccgcaaaag cagcgatgac tgttgctgcg ctgtgtgaaa
    39121 agcataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa accgaacgtt
    39181 tctatctcga cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg
    39241 aagctggcgc gtcggtttac tcggcttccg agctggcagc gcaggagttt ccggatctcg
    39301 acgtttcgct gcgtggcgcg gtgtctatcg cccgccgttt gcaggatccg ctggcggagc
    39361 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    39421 cgcaactggc ccgcaaactg gacgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    39481 atctcaacac tgcttctgtt ccgctgttaa cccgcgtggc gggcctgacg cgcatgatgg
    39541 cgcaaaacat cgtggcctgg cgcgatgaga acggtcagtt ccagaaccgt cagcaactgc
    39601 tgaaagtgag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ttgcgcatta
    39661 accatggtga taacccgctg gacgcgtcta ccgtccaccc ggaagcctat ccggtggtgg
    39721 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    39781 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    39841 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    39901 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggtg
    39961 cggtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat cggcgtgcat caggacggcc
    40021 tggttcacat ctcttcattg tcgaacaagt ttgtagaaga tccgcatacc gtggtgaaag
    40081 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    40141 tgaccatgcg tctggacgag cagcctggcg aaaccaacgc ccgtcgcggc ggcggtaatg
    40201 aacgcccgca aaacaaccgc ccggcagcca aaccgcgcgg acgtgaagcg cagcctgccg
    40261 gtaacagcgc gatgatggac gcgctggcgg cagcaatggg caaaaaacgt taaacgccct
    40321 gattgacccc taaacctgcg cgataaggtg tccacgccgc atctggcaac gaacgccttg
    40381 tctgacataa aagtgccgga gaatatctcc ggcattttta ttccacagcc aaactcacaa
    40441 tatattccgg caacatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    40501 gtcctggtaa ttcgctattc gaattatatt ttcgctgcga tataaccttg agccacatca
    40561 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    40621 aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca
    40681 tctggtctca tgtcgctgtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    40741 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    40801 aatcagcccg gcatatcgcc aaaaactgct ttctcttggc atgttacctg gctcctcttt
    40861 taatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    40921 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    40981 cggtgataac aacaatgaaa aaattaacca taggcttaat tggtaatcca aattctggca
    41041 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    41101 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    41161 acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa
    41221 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    41281 ctaaccttga gcgtaacctg tacctgacgc tacaactgct ggaactcggc attccctgca
    41341 tcgtggcact gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    41401 ctctgtcggc gcgtcttggc tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta
    41461 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    41521 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    41581 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    41641 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg
    41701 agatggacga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    41761 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    41821 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    41881 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg
    41941 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    42001 ctatcttcct cgcccagggc ctgggtggcg gcattaacac cgtgctgcca ctggtgccgc
    42061 agattggcat gatgtacctg ttcctctcct tccttgagga ctccgggtat atggcgcgtg
    42121 cggcgtttgt gatggaccgt ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    42181 cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg
    42241 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    42301 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcg ctggcggtct
    42361 tctcgctgta tatgctgggt attgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    42421 ccatcatgcg cggtgaagcg acgccgtttg tcatggagtt gccggtctat catgtaccac
    42481 acgttaaaag cctgattatc cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg
    42541 gtaaagtgat catcatcgtc agcattttcc tgagcgcttt caacagcttc tcgctgagcg
    42601 ggaaaatcgt cgataacatc aacgactcgg cgctggcgtc cgtcagccgg gtgatcaccc
    42661 cagtcttcaa gccaattggc gtgcatgaag ataactggca ggcaacggtt ggcctgttta
    42721 caggtgccat ggcgaaagaa gtggtagtgg gtacgctcaa caccctctac accgcagaaa
    42781 atattcagga cgaagagttc aatccggcag agtttaacct cggtgaagag ctgttcagtg
    42841 cgatagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    42901 ccattgaagc cagcaaaggc gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc
    42961 agaaattcgg tagcgcagca gcagcttaca gctacctgat tttcgtcctg ctgtatgtac
    43021 catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct
    43081 ccatcctgtg ggggctgaat atcgcttact cgctggcaac attgttctat caggtcgcca
    43141 gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggttatc ctgtttaaca
    43201 tcgtggttat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    43261 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    43321 ttcacttatt caggtgcgcg atttgctggc gttacggggc cgtatggaag cggcccagat
    43381 aagccagaca ttgaacactc cacagccaat gattaacgcc atgctgcaac aactggaaag
    43441 tatgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    43501 aagctgcccg gaaggaaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccttactc
    43561 catcgcctgt tttggaaagc ccggtatgcg tctgcatccg ggcttttttg cgtgcggctt
    43621 tccataaaaa tgcaactctt gcagcacggc gtaagttcct ttgaaagcat ctcgcaggga
    43681 tgaaaactcg ctaatacaca ggtgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca
    43741 ggtgaaggaa cgccatgagc aaaaagcaga gttccacccc acacgatgcg ctgttcaaac
    43801 tctttttacg ccaaccggac acggctcgtg attttcttgc gtttcattta ccggcaccca
    43861 ttcacgcgct ttgtgatatg aaaaccctca agctggagtc gagcagcttt attgatgacg
    43921 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    43981 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    44041 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    44101 tggtggtgct attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtctt
    44161 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    44221 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    44281 tggagttaat tcaaaaacat attcgtcaac gcgacctgat ggggctggta gagcaaatgg
    44341 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    44401 tactgcaaac cggcgacgcg gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    44461 caccgaaaca caaggagagt ttaatgacaa ttgcggaaag attgcggcag gagggggaac
    44521 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttacagaca
    44581 tcatgcgctt tactggggtg tcagaagaag agttggctgc ggcgagccag taaatttctg
    44641 tctcgccatt tcaaaagttg cctacaccct ctgcttcaac gccaccagca ggtgacaaaa
    44701 ctcgaccgga tgcgaaataa acggcgcatg ggccgctttg gcgaagatat atgattcgct
    44761 gtgaggccaa agtttatcca gcatcggcac cactttgcgc ggcaccagac cgtcgagata
    44821 gccatacaat cgcaaaaacg gcatggacac gttttgcagc ggctgacgga gatcgaccgt
    44881 tttcaggatt tccagcccgc cattaagcac gtcaacctcc ggcatcggta acgccagaac
    44941 ggttttcttc aacgcccgcg catcctggcg cgccgtttca gtccccatgg tttgtaacgc
    45001 caggaaccgc tccactgtac gctgaaaatc atcactgagt tgctgctgaa atcccgccag
    45061 cacgtccggt tttatccccg gccactcgtc acgagcacta aaacaaggtg acgacgccac
    45121 ggtgaccagc gcctgaacac gctcgggatg ggttaacgca atctggcttg ccaccagccc
    45181 gcccagactc cagcctaacc aaatggcttt atcaggtgcc tgttgcagca cggcttcggc
    45241 catatcagca agtgacagcg caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac
    45301 aaggtgcagc gtaaaatgcg agctaagttc ctcgtcaatg caacgccaca cttcggcatt
    45361 cagtccccat ccgtgcagca gcacaagatg aacattcccc tgacctttgg tctgccacca
    45421 gatgttattc atccgctatt gttctctttt gacttacaag gatgaacata tgctaacagt
    45481 accgggatta tgctggctat gccgaatgcc actggcgtta ggtcattggg ggatttgttc
    45541 ggtctgttca cgcgccaccc gcacagataa aacgttatgc ccacaatgtg gattacccgc
    45601 cacacactcc catcttccct gcggtcgctg cctgcaaaaa ccgccgccct ggcaaagact
    45661 ggtcacagtt gccgactatg cgccgccgtt aagtctgctc atccaccagc ttaaattttc
    45721 ccggcgcagt gaaatcgcca gcgccctgtc acgtctgtta ctattggaag tcttgcacgc
    45781 tcgtcgcacc accgggttgc aattgccgga tcgcatcatc agcgttccgt tatggcagcg
    45841 gcgtcactgg cgtcggggat ttaatcagag cgatttgctg tgtcagccgt tatcacgctg
    45901 gttgcactgc caatgggata gcgaagccgt cacacgtaca cgggccactg cgacccagca
    45961 ttttctcagt gcccggctgc gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc
    46021 cgtgcaaggt cgccatatgg tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc
    46081 agagattgcg cagttgcttt tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg
    46141 tcgaaccttg tagagcctcg atgatgggcg tattataacc aactaaaata gtcaactatt
    46201 aggccattac tatgatccgt atttccgatg ctgcacaagc gcactttgcc aaactgctgg
    46261 caaatcagga agaagggaca caaatccgcg tatttgtgat taaccctggc acgcctaacg
    46321 ctgaatgtgg tgtttcttat tgtccgccgg atgccgtgga agccaccgac actgccctga
    46381 aatttgacct gctgaccgcg tatgttgatg agttaagcgc accatacctg gaagatgcag
    46441 agatcgactt tgttaccgac cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca
    46501 aaatgcgtaa agtggcagac gatgcaccgc tgatggagcg cgtggagtat atgctgcagt
    46561 cgcagatcaa cccacagctt gctggtcacg gtggtcgcgt ttcgctgatg gaaatcaccg
    46621 aagacggtta cgccattctg caatttggcg gcggctgtaa cggttgttcc atggtcgatg
    46681 tgacgctgaa agaagggatc gagaagcagc tgctaaacga attcccggag ctgaaaggtg
    46741 tgcgcgatct caccgaacac cagcgcggcg aacactccta ctactaagtt atcccctctt
    46801 ttgtagattg cccgatgcga cgctaaagcg tcatttcggg cctacgttga acatgtgcca
    46861 gacgttggcg cagcattgct ttggcaaatc cctcccttcg tttctccgcg ataatatgac
    46921 caacctctca taatttaaat ttaccccgct ctggtgattc tcaaacgcca gatgttaccc
    46981 gtatcattca catgggtacc aaacatactc ctgacatctg actacaataa ttagttttag
    47041 tgggtatcag tcgtggtgcc gcaatatctc tgttcccgat tgggataatt agagtttgtc
    47101 gtcagaaaat tgacgttacc cataacaaat gaaaggccag gtaaatcatg ccattagtca
    47161 ttgttgctat cggtgtaatc ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct
    47221 tcatcgctct cgtcctcgtg gcgcttgctg ttggattaat gcaaggaatg ccgctggata
    47281 aagttattgg ctccatcaaa gccggtgtcg gcgggacgct cggtagcctt gccctgatca
    47341 tgggttttgg cgcaatgctg ggcaaaatgc tggcagactg cggtggcgca caacgtatcg
    47401 ccaccacgct gattgccaaa tttggtaaaa aacacatcca gtgggcggtg gtactgaccg
    47461 gttttaccgt tggttttgcc ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg
    47521 tgtttaccat cgcggcttct gcgaatattc cactgttgta tgttggtgta ccaatggcag
    47581 ctgcactgtc tgtgacccac ggcttcctgc caccgcatcc gggtccgact gcgattgcca
    47641 ccattttcaa tgccgatatg ggtaaaaccc tgctgtacgg tactattctg gcaatcccga
    47701 ccgtgattct cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat aagccaattc
    47761 cggaaggtct ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc tttggcgtca
    47821 gcgtctggac ctctctggtg ccggtagtac tgatggcgat gcgtgcgatt gccgaaatga
    47881 tcctgccgaa aggtcacgct ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg
    47941 ctacgctgat tgccgtgctg attgccatgt tcacctttgg tctgaaccgt ggtcgttcaa
    48001 tggatcagat taacgacacg ctggtttctt ccatcaaaat cattgcgatg atgctgttga
    48061 tcatcggtgg tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg gacaaataca
    48121 ttgcttccat gatgcacgaa accaacattt ctccgctgct gatggcctgg tcgattgctg
    48181 ccgtactgcg tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg
    48241 cggcaccgct gattgcaacg acgggtgtta gcccggagct gatggttatt gcggtgggtt
    48301 ccggtagtgt gattttctct catgtgaacg atccgggctt ctggctgttc aaagagtact
    48361 ttaacctgac tatcggcgag accatcaaat cctggtcgat gctggaaacc attatctcgg
    48421 tgtgcggtct ggtaggctgt ctgctgctga atatggtgat ttgataacac tgactgccgg
    48481 atgcatcgca tccggcatca accgcactct acttcttctt cgctgcagct ctgcgccgtc
    48541 tgtccaaatc cttcagcaac ttgttcacgc catcatcggc aaacatcgac tcaagcgttg
    48601 cggaaagctt gcgtcgccag tttttatact ggtaactggt gccaggaata ttcaccggtt
    48661 cggccatatc cagccagtct tccggctgta gtcctaacag agcactgtta ctgtcggcaa
    48721 tgtagcgctg caaaccacgg ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc
    48781 cggcacgttt tggcagacaa ccatatttat gcagtgcatc cagcagccct tgcttcgcca
    48841 gttcgcgatc ctgatacaga ccgcgcagta ccacttcatc cggatacagc cccagggttt
    48901 tgcccagcgt tagatccccg ctctcccagt aaccgcgtag cgtaggcagg tcatgtgtcg
    48961 ccgcaaccgc catcgactgc tccggatacg ctttcggtgc acggaacgtc ttctcgtggt
    49021 cgttttcgaa atagagcact ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa
    49081 tctctaccgg tacggtaccg agatcttcac caatcaccat acagcgatga cgtttacttt
    49141 cgagtgccag aatcgagagc agatcatcca ccggatagtg aacatacgcg ccctgatctg
    49201 ccgtctcgcc atacggtatc caccacaaac gcagcatcga catcacatgg tcaattcgta
    49261 atgcgccgca gttttgcata ttggcacgca acagctcgat aaacggttca taggcacgcg
    49321 cggtgatgat atgcgggtcc attggcggta atccccagtt ctgccccaac gggccgagga
    49381 tatccggcgg cgcgccaacc gacgctttca ggcaatatag ttcacgatca caccaggttt
    49441 ccgccccacc ttccgctacg ccaaccgcca gatcacgata caagccaatc ggcatttcat
    49501 agccctggct tatctcccag caggcggcaa actggctgta agccagccac tgcaaccaga
    49561 gataaaaatc gacgtcatta cgatgttctt cgcagaactg acgcacttct ggtgaatcca
    49621 cgttctgata catctctggc catgcaggcc agccccagcg catttcgtcc tctttcactt
    49681 gctgggcatg tagcgcatca aaggctgcct gccagaacag gctgtcgccc tgctctgcaa
    49741 caaactggcg aaacgcggtc atctgctcat catcacgttg cgcgaaacct ttccacgcca
    49801 ttcgtaatgc tgtcattttt agggcggtaa ccgtggagta atcgacccag tcggcatcgc
    49861 gcgcctgttg cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc
    49921 taagatggaa atcttcaacg gcgttaacgt cgatataaat cacattcagc caacggcgag
    49981 aagacgggct gtatgggctg gcactctccg gatttaccgg atagagcgca tgaatcgggt
    50041 tcaggccgat gaacgacccg ccacgttttg ccacatccac cagcatcgct ttgagatcgc
    50101 caaaatcccc aataccccag tttttttccg atcgcagcgt ataaagctga acgcaggcac
    50161 cccacagctt ttgtttattc agcaacgcct gcggttcgta acagcgtttc ggggcgacaa
    50221 tcacccggca atgcgcgcgc tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg
    50281 gcagcttcgt cggtagattg aacgctttgc cccccgttac atggcctttg tactgtgttc
    50341 cttcttcggt ggtcagcagc cagctatatt cgccgctgcc ctccaccacc atcggcattt
    50401 ttttgccgct ggtataaacc atgacattcg ggactggcgt taccgccact ttcgtggcgg
    50461 tacgttgatg catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt
    50521 taccgtgggc attgatgtaa ttggggctaa tccccgccgc cagcgcggca ttatccagac
    50581 gtttgctttc catcgagctt ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc
    50641 gaatagagcg atccgagcta aacataccgc agcgggcggt attgaggatc gccgcgcgag
    50701 tccaggcctc ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca acataggctg
    50761 cgaaatccgc catcaccaga tacggatcgc cgccctgttt gccgatactg tgcagcatct
    50821 ggtcgaaggc atgcttatcg ccgtcgctgt atttaccgct ttccagctct ttcaatactg
    50881 cgtccagcac cttatctttc ttccgccatt tcaccgggtc gtagcctttg gccagaatag
    50941 ccttcacttg ttccacggta tgaccaaaaa taaagatatt ttcttcaccg actttctcgg
    51001 cgatttcaac gttcgcacca tccagcgtac cgacagtaag cgcaccattg agcgccagtt
    51061 tcatattgcc ggtaccggaa gcttctttac ctgcagtcga aatttgttcg gagatatccg
    51121 ccgccgggat cagtttttcc gccgccgaaa cgcaataatc cggcaggaac accaccttca
    51181 acttatcgcc aaccagtgga tcgttgttga tcacgtcagc cactttgttg atcgcaaaga
    51241 taatattctt cgccaggtag tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg
    51301 gtacgcgatc agcctgcggg ttttcacgaa tttctttgta caacgccaga atatgcagca
    51361 gattcaggtg ctggcgtttg tactcatgca gacgtttgat ctgaatatcg aaaatcgcct
    51421 gtggattgat ctcaataccg gtacgaactt tcacaaactc cgccagacgg actttattcg
    51481 cctgcttgat ctcgcgatat tgctgacgga atttcgcatc atcagcgaat ttttccagat
    51541 tgatcagctg atcgagatcg ttagcccact ctttttgcag tgatttatcc aacagagccg
    51601 ccagtgcagg gttgcactgt ttgatccagc gacgtggggt aataccgttg gtgacgttat
    51661 ggaatttgtt cggccatagc tggtgatatt ccgggaacag atctttcacc accagatccg
    51721 agtgcagcgc cgcaacaccg ttcaccgcga aaccgccaac cacacacagg ttcgccatat
    51781 gcacttgttt atcgtgcacc accgccagtt tggcccacac tttttcatcg cccggccagg
    51841 ttttctccac cagcgtttta aagcgagtat taatttcgtt aataatctgc atatggcgcg
    51901 gcagtaagcc tttcaccagt ttcacatccc agcgttccag cgcttctggc atcagggtat
    51961 ggttggtgta agcgaaagtt ttgctggtaa tggcccaggc gtcatcccag ctcatctggt
    52021 gctcatcgat cagcacgcgc agcagttctg gaatcgcgat agttgggtgg gtatcgttca
    52081 gctgaataac ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga tgatggcgac
    52141 gcaaaatatc cgctaccgaa caggcacact ggaagtgctg ctgcatcaag cgcagctttt
    52201 taccggcagt atggttgtcg tttggataga gaactttggt cagtttttcc gcattgatgc
    52261 cctgctgttc ggcacgcagg aaatcaccgt cgttaaattt agtcagatca aacggatgcg
    52321 cgtgcgtcgc ctgccacaga cgcagcggct gcgccacgcc attacgatag ccgacaacgg
    52381 ggagatccca cgcttgaccg gtaatggtaa actccggctc ccagcgtccg tctttcgtca
    52441 ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt
    52501 tactgcggtg ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga
    52561 acaaaccata ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg
    52621 agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt acccagcgcc gggtcgatct
    52681 cttcttccag caggtccgtc agattgatgt cataagcctt caacgaatcc tgtacatcct
    52741 gataccagcc gagattcaac aggttgttgc ccgtcaggcg accaatcaaa aactccattg
    52801 agatgtagtt aacatgtcgc tgattcgcca ccggcttggc gaatggctga gcacgcagca
    52861 tttcggccag tgcttcactc actgctagcc accactggcg aggagtcatt tcagccgcag
    52921 aatttaagcc ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa
    52981 aaataggttg tgacatagga gttccacttt tcttagattt tcaacacaac gttatcgcta
    53041 gtttgccagg ctcgatgttg accttcctca tcctgcgggg gattaggcag ggaggagttg
    53101 cggggatgag caaggaaatg tgatctcaac cacttaaagc tagtgcaaac cacaggatta
    53161 gcagcaaatc aatgcaatac agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt
    53221 gcgtattttc aaaaagcgga aggtaactct ataaattaag taaaggagtg aaacagtttc
    53281 ataagtaaaa tatccagtgt gctccatctc attcttaata gatttattaa gatcatcttt
    53341 ttagatggca ctttcatcag gaatgaagaa gaaacccttg cttaaatgaa tctgatgaac
    53401 ataagggaaa ccagtattca cgctggatca gcgtcgtttt aggtgagttg ttaataaaga
    53461 tttggaattg tgacacagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag
    53521 aaaggtttct ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc
    53581 tcattttcca cagtgaagtg attaactatg ctgattccgt caaaactaag tcgtccggtt
    53641 cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac
    53701 ttccggctgg cgctgatcac gagtcctgcg ggctacggaa agaccaccct catttcccag
    53761 tgggcggcag gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa
    53821 gagcgtttcg ccagctatct cattgccgcc gtgcagcagg caaccaacgg tcactgtgcg
    53881 atatgtgaga cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag
    53941 cttttcattg agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat
    54001 ctgatcacta atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa
    54061 aatctcaccc tggtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt
    54121 gttcgtgatc aactgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg
    54181 aagcagtttt ttgattgccg tctgtcatcg ccgattgaag ccgcagaaag cagtcggatt
    54241 tgcgatgacg tttccggttg ggcgacggca ctacagctaa tcgccctctc cgcccggcag
    54301 aatacccact cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt
    54361 tcggattatc tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg
    54421 ttgaaaagcg ccattttgcg ctcaatgaac gatgcactca tcacccgtgt gaccggcgaa
    54481 gaaaacgggc aaatgcgcct cgaagagatt gagcgtcagg ggctgttttt acagcggatg
    54541 gatgataccg gcgagtggtt ctgctatcac ccgctgtttg gcaacttcct gcgccagcgc
    54601 tgccagtggg aactggcggc ggagctgccg gaaatccacc gtgccgccgc agaaagctgg
    54661 atggcccagg gatttcccag cgaagcgatt catcatgcgc tggcggcagg cgatgcgctg
    54721 atgctgcgcg atattctgct taatcacgcc tggagtctgt tcaaccatag cgaactgtcg
    54781 ctgctggaag agtcgcttaa ggccctgccg tgggacagct tgctggaaaa tccgcagttg
    54841 gtgttattgc aggcgtggct gatgcaaagc caacatcgct acggcgaagt taacaccctg
    54901 ctagcccgtg ctgaacatga aatcaaggac atcagagaag acaccatgca cgcagaattt
    54961 aacgctctgc gcgcccaggt ggcgattaac gatggtaatc cggatgaagc ggaacggctg
    55021 gcaaaactgg cactggaaga gctgccgccg ggctggttct atagccgcat tgtggcaacc
    55081 tcggtgctgg gtgaagtgct gcactgcaaa ggcgaattga cccgctcact ggcgctaatg
    55141 cagcaaaccg aacagatggc acgccagcac gatgtctggc actacgcttt gtggagttta
    55201 atccagcaaa gtgaaattct gtttgcccaa gggttcctgc aaaccgcgtg ggaaacgcag
    55261 gaaaaagcat tccagctgat caacgagcag catctggaac agctgccaat gcatgagttt
    55321 ctggtgcgca ttcgtgcgca gctgttatgg gcctgggcgc ggctggatga agccgaagcg
    55381 tcggcgcgta gcgggattga agtcttgtcg tcttatcagc cacagcaaca gcttcagtgc
    55441 ctggcaatgt tgattcaatg ctcgctggcc cgtggtgatt tagataacgc ccgtagccag
    55501 ctgaaccgtc tggaaaacct gctggggaat ggcaaatatc acagcgactg gatctctaac
    55561 gccaacaaag tccgggtgat ttactggcaa atgaccggcg ataaagccgc cgctgccaac
    55621 tggttgcgtc atacggctaa accagagttt gcgaacaacc acttcctgca aggtcaatgg
    55681 cgcaacattg cccgtgcaca aatcttgctg ggcgagtttg aaccggcaga aattgttctc
    55741 gaagaactca atgaaaatgc ccggagtctg cggttgatga gcgatctcaa ccgtaacctg
    55801 ttgctgctta atcaactgta ctggcaggcc ggacgtaaaa gtgacgccca gcgcgtgttg
    55861 ctggacgcat taaaactggc gaatcgcacc ggatttatca gtcattttgt catcgaaggc
    55921 gaagcgatgg cgcaacaact gcgccagctg attcaactca atacgctgcc ggaactggaa
    55981 cagcatcgcg cacagcgtat tctgcgagaa atcaatcaac atcatcggca taaattcgcc
    56041 catttcgatg agaatttcgt tgaacgtctg ctaaatcatc cggaagtgcc agaactgatc
    56101 cgcaccagcc cattgaccca acgtgaatgg caggtcctgg ggctgattta ctccggttac
    56161 agcaatgaac aaattgcggg cgaactggaa gttgcggcaa ccaccatcaa gacgcatatc
    56221 cgcaatctgt accaaaaact cggcgtcgcc caccgtcagg ctgccgtgca gcacgcgcag
    56281 aaactgctga agatgatggg ttatggggtg tgagtttagc cggataatgc attggatccg
    56341 gtttacatct ttacatcatt caatactcac ccgcgttacg ccatctgttt ctatcaaacc
    56401 aaaccgcacc ggcaagaaac gctccaccac cgcgatattg gtcagcagat ggcatgaagg
    56461 atgggcgacc ttaaattctc ccgcgcccgc cagcgccatc ggcagtacca gttggtcggc
    56521 gagatattcc cccaccgccg ctgggcttgc caggtagcgt ttcacctctt tcactaactg
    56581 cgcggctacc acctcggcac tgacgcgctt ttcaccgacg acgaaaaagc gttcagtgat
    56641 attttcactt tcgacttcca gcgagacggt attacccggc ccctggtcgc gcggcaggtt
    56701 atgaatattc tgttcatgca gggaaaaact ccccgccagt gtagcgattt cacgctcagc
    56761 aacatggcgc ggcacaccag ctaatagaac ttctccacac atctgcacaa tgttcccgcg
    56821 ctcgccaagt tgcaaggtgt taaacgatgc cacgggcgag acttccgttg ccaccacacc
    56881 gcctccggca ggataaaaac cgtgacgtaa cagcgtggtt tgctgatgaa ttcctatttt
    56941 cgccagcagc ggctccagca cccggcggat aaaatccgca ggcggggccg acgggttatc
    57001 ggtgcctccg ctcacttcaa cacgcgaagg tccatcggca aaccacagcg cgggcagcac
    57061 cgtttgcagc accagcgtac agcttccggc actaccgata gcaaagcggt actcgccgcc
    57121 gcgcacggtg ccaggccgga agaccagacg ctgcgacccc agctccgcgc cttccaccgt
    57181 tgccctacaa atttccgcag ccgcttttac cgcggtcaga tgctggcgca acagccccgg
    57241 tttcgctcgc ccggcacgaa tgctggtgat ggtaaatggc tggccggtta tcatcgacag
    57301 gctcagcgcc gagcgcagga tctgcccgcc accttcgccc tgtgcgccat ccagcgcaat
    57361 catccttttc atcatttatc cttttacgca caccacctga cgcagggtat agataacttc
    57421 caccagatcg ctttgtgccg ccatcaccgc atcaatatct ttatacgcca tcgggatttc
    57481 gtcgatcact tcggcatctt tacggcattc cacatgcgcg gtggcacgaa tttgatcttc
    57541 cacgctgaac agttttttcg ctttagtacg gctcattacc cgcccggcac cgtggctgca
    57601 cgaacagaac gactcttcat ttcccagccc acggacgata aagcttttcg ctcccatcga
    57661 accgggaata attccatatt gaccagcacg cgcagacacc gcgcctttac gcgtcacgta
    57721 gatctcttcg ccaaagtgct gttctttttg cacatagttg tggtgacagt tgatctcttc
    57781 catcgccagc gtttgctgtt gtcttaccgt tttctgcgta acgctctgca acgctgttac
    57841 tacgttttcc atcattgcat cgcggttaag gctggcaaaa agctgcgccc aggccaccgc
    57901 tttcaggtaa tcatcaaagt attccgtacc ttccataaag tacgccagat cacgcgacgg
    57961 caacgtttca agctggtcct gcatctcttt ttgcgccaga tcgataaagt aagtcccgat
    58021 ggcgttacca attccgcgtg aaccggagtg cagcataatc cacacctgat ccgactcatc
    58081 aagacagatt tcaataaaat ggttacccgt tcccagcgtt cccagatgtt tatagttatt
    58141 ggtattcagg aaacgcggat atttttgcgt taaccactga taaccggctt ccagctcagc
    58201 ccatttagca tcgacgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg
    58261 cccaccgccg ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct
    58321 ttacccaggt gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc
    58381 gtattaataa gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt
    58441 accggggcat tttcagtggg cagtaattcg taattcattt tgtttttctc ttttcgttgt
    58501 ttgc
//
LOCUS       DAZFFI010000017        57768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFFI010000017 DAZFFI010000000
VERSION     DAZFFI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.017"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..171)
                     /locus_tag="ACHJ12_001286"
     CDS             complement(<1..171)
                     /locus_tag="ACHJ12_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4989107.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMAS"
     gene            179..680
                     /gene="tnpA"
                     /locus_tag="ACHJ12_001287"
                     /pseudo
     CDS             179..680
                     /gene="tnpA"
                     /locus_tag="ACHJ12_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(698..1366)
                     /gene="ydcL"
                     /locus_tag="ACHJ12_001288"
     CDS             complement(698..1366)
                     /gene="ydcL"
                     /locus_tag="ACHJ12_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4989108.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1669..2262)
                     /gene="tehB"
                     /locus_tag="ACHJ12_001289"
     CDS             complement(1669..2262)
                     /gene="tehB"
                     /locus_tag="ACHJ12_001289"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4989109.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2259..3251)
                     /gene="tehA"
                     /locus_tag="ACHJ12_001290"
     CDS             complement(2259..3251)
                     /gene="tehA"
                     /locus_tag="ACHJ12_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4989110.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3375..4355
                     /gene="ydcK"
                     /locus_tag="ACHJ12_001291"
     CDS             3375..4355
                     /gene="ydcK"
                     /locus_tag="ACHJ12_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4989111.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4350..4886)
                     /gene="rimL"
                     /locus_tag="ACHJ12_001292"
     CDS             complement(4350..4886)
                     /gene="rimL"
                     /locus_tag="ACHJ12_001292"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO4989112.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4949..5173)
                     /gene="ydcH"
                     /locus_tag="ACHJ12_001293"
     CDS             complement(4949..5173)
                     /gene="ydcH"
                     /locus_tag="ACHJ12_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4989113.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5313..6968)
                     /gene="opgD"
                     /locus_tag="ACHJ12_001294"
                     /gene_synonym="mdoD"
     CDS             complement(5313..6968)
                     /gene="opgD"
                     /locus_tag="ACHJ12_001294"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4989114.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7193..8536)
                     /gene="ydcJ"
                     /locus_tag="ACHJ12_001295"
     CDS             complement(7193..8536)
                     /gene="ydcJ"
                     /locus_tag="ACHJ12_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4989115.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8753..9676
                     /gene="ydcI"
                     /locus_tag="ACHJ12_001296"
     CDS             8753..9676
                     /gene="ydcI"
                     /locus_tag="ACHJ12_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4989116.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGVRLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9714..11354)
                     /gene="trg"
                     /locus_tag="ACHJ12_001297"
     CDS             complement(9714..11354)
                     /gene="trg"
                     /locus_tag="ACHJ12_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4989117.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVIRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11753..11902
                     /gene="hokB"
                     /locus_tag="ACHJ12_001298"
     CDS             11753..11902
                     /gene="hokB"
                     /locus_tag="ACHJ12_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4989118.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11880..11948)
                     /gene="yncP"
                     /locus_tag="ACHJ12_001299"
     CDS             complement(11880..11948)
                     /gene="yncP"
                     /locus_tag="ACHJ12_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4989119.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11974..12147)
                     /locus_tag="ACHJ12_001300"
     CDS             complement(11974..12147)
                     /locus_tag="ACHJ12_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989120.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12392..12922)
                     /gene="cybB"
                     /locus_tag="ACHJ12_001301"
     CDS             complement(12392..12922)
                     /gene="cybB"
                     /locus_tag="ACHJ12_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4989121.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13111..14112
                     /gene="gap"
                     /locus_tag="ACHJ12_001302"
     CDS             13111..14112
                     /gene="gap"
                     /locus_tag="ACHJ12_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4989122.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYSDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14154..15593)
                     /gene="aldA"
                     /locus_tag="ACHJ12_001303"
     CDS             complement(14154..15593)
                     /gene="aldA"
                     /locus_tag="ACHJ12_001303"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4989123.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15790..16590)
                     /gene="ydcF"
                     /locus_tag="ACHJ12_001304"
     CDS             complement(15790..16590)
                     /gene="ydcF"
                     /locus_tag="ACHJ12_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4989124.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16862..20764)
                     /gene="hrpA"
                     /locus_tag="ACHJ12_001305"
     CDS             complement(16862..20764)
                     /gene="hrpA"
                     /locus_tag="ACHJ12_001305"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4989125.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20965..21570
                     /gene="azoR"
                     /locus_tag="ACHJ12_001306"
     CDS             20965..21570
                     /gene="azoR"
                     /locus_tag="ACHJ12_001306"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4989126.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21621..22937)
                     /gene="ynbD"
                     /locus_tag="ACHJ12_001307"
     CDS             complement(21621..22937)
                     /gene="ynbD"
                     /locus_tag="ACHJ12_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO4989127.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22927..24684)
                     /gene="ynbC"
                     /locus_tag="ACHJ12_001308"
     CDS             complement(22927..24684)
                     /gene="ynbC"
                     /locus_tag="ACHJ12_001308"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4989128.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24700..25596)
                     /gene="ynbB"
                     /locus_tag="ACHJ12_001309"
     CDS             complement(24700..25596)
                     /gene="ynbB"
                     /locus_tag="ACHJ12_001309"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4989129.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTSQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25596..26201)
                     /gene="ynbA"
                     /locus_tag="ACHJ12_001310"
     CDS             complement(25596..26201)
                     /gene="ynbA"
                     /locus_tag="ACHJ12_001310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4989130.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRFGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(26372..28678)
                     /gene="ydbD"
                     /locus_tag="ACHJ12_001311"
     CDS             complement(26372..28678)
                     /gene="ydbD"
                     /locus_tag="ACHJ12_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO4989131.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIIR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQKLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28742..29602)
                     /gene="pdxI"
                     /locus_tag="ACHJ12_001312"
     CDS             complement(28742..29602)
                     /gene="pdxI"
                     /locus_tag="ACHJ12_001312"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4989132.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29833..30423)
                     /gene="paaY"
                     /locus_tag="ACHJ12_001313"
     CDS             complement(29833..30423)
                     /gene="paaY"
                     /locus_tag="ACHJ12_001313"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO4989133.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(30405..31355)
                     /gene="paaX"
                     /locus_tag="ACHJ12_001314"
     CDS             complement(30405..31355)
                     /gene="paaX"
                     /locus_tag="ACHJ12_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO4989134.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31456..32769)
                     /gene="paaK"
                     /locus_tag="ACHJ12_001315"
     CDS             complement(31456..32769)
                     /gene="paaK"
                     /locus_tag="ACHJ12_001315"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO4989135.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32796..34001)
                     /gene="paaJ"
                     /locus_tag="ACHJ12_001316"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32796..34001)
                     /gene="paaJ"
                     /locus_tag="ACHJ12_001316"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO4989136.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34001..34423)
                     /gene="paaI"
                     /locus_tag="ACHJ12_001317"
     CDS             complement(34001..34423)
                     /gene="paaI"
                     /locus_tag="ACHJ12_001317"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO4989137.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34413..35840)
                     /gene="paaH"
                     /locus_tag="ACHJ12_001318"
     CDS             complement(34413..35840)
                     /gene="paaH"
                     /locus_tag="ACHJ12_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO4989138.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35842..36630)
                     /gene="paaG"
                     /locus_tag="ACHJ12_001319"
     CDS             complement(35842..36630)
                     /gene="paaG"
                     /locus_tag="ACHJ12_001319"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO4989139.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36630..37397)
                     /gene="paaF"
                     /locus_tag="ACHJ12_001320"
     CDS             complement(36630..37397)
                     /gene="paaF"
                     /locus_tag="ACHJ12_001320"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO4989140.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37394..38464)
                     /gene="paaE"
                     /locus_tag="ACHJ12_001321"
     CDS             complement(37394..38464)
                     /gene="paaE"
                     /locus_tag="ACHJ12_001321"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO4989141.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38472..38969)
                     /gene="paaD"
                     /locus_tag="ACHJ12_001322"
     CDS             complement(38472..38969)
                     /gene="paaD"
                     /locus_tag="ACHJ12_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO4989142.1"
                     /translation="MQRLATIAPPQVHEIWELLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38984..39730)
                     /gene="paaC"
                     /locus_tag="ACHJ12_001323"
     CDS             complement(38984..39730)
                     /gene="paaC"
                     /locus_tag="ACHJ12_001323"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO4989143.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39739..40026)
                     /gene="paaB"
                     /locus_tag="ACHJ12_001324"
     CDS             complement(39739..40026)
                     /gene="paaB"
                     /locus_tag="ACHJ12_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO4989144.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40038..40967)
                     /gene="paaA"
                     /locus_tag="ACHJ12_001325"
     CDS             complement(40038..40967)
                     /gene="paaA"
                     /locus_tag="ACHJ12_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO4989145.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41252..43297
                     /gene="paaZ"
                     /locus_tag="ACHJ12_001326"
     CDS             41252..43297
                     /gene="paaZ"
                     /locus_tag="ACHJ12_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO4989146.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIAGAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43545..45818
                     /gene="tynA"
                     /locus_tag="ACHJ12_001327"
     CDS             43545..45818
                     /gene="tynA"
                     /locus_tag="ACHJ12_001327"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO4989147.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRLHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45876..47375)
                     /gene="feaB"
                     /locus_tag="ACHJ12_001328"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45876..47375)
                     /gene="feaB"
                     /locus_tag="ACHJ12_001328"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO4989148.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47611..48516
                     /gene="feaR"
                     /locus_tag="ACHJ12_001329"
     CDS             47611..48516
                     /gene="feaR"
                     /locus_tag="ACHJ12_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO4989149.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48688..49017)
                     /gene="ydbL"
                     /locus_tag="ACHJ12_001330"
     CDS             complement(48688..49017)
                     /gene="ydbL"
                     /locus_tag="ACHJ12_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4989150.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GNWVRKF"
     gene            complement(49022..49207)
                     /gene="ynbE"
                     /locus_tag="ACHJ12_001331"
     CDS             complement(49022..49207)
                     /gene="ynbE"
                     /locus_tag="ACHJ12_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4989151.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49204..51843)
                     /gene="ydbH"
                     /locus_tag="ACHJ12_001332"
     CDS             complement(49204..51843)
                     /gene="ydbH"
                     /locus_tag="ACHJ12_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4989152.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            52051..53040
                     /gene="ldhA"
                     /locus_tag="ACHJ12_001333"
     CDS             52051..53040
                     /gene="ldhA"
                     /locus_tag="ACHJ12_001333"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4989153.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53151..53573
                     /gene="hslJ"
                     /locus_tag="ACHJ12_001334"
     CDS             53151..53573
                     /gene="hslJ"
                     /locus_tag="ACHJ12_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4989154.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53570..53836)
                     /gene="ydbJ"
                     /locus_tag="ACHJ12_001335"
     CDS             complement(53570..53836)
                     /gene="ydbJ"
                     /locus_tag="ACHJ12_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4989155.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54110..57634
                     /gene="nifJ"
                     /locus_tag="ACHJ12_001336"
     CDS             54110..57634
                     /gene="nifJ"
                     /locus_tag="ACHJ12_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4989156.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSTKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14593 a        15187 c        13752 g        14236 t
ORIGIN      
        1 ggaagccatt ttcccgtaag ggatgtattt attaccggcc tcatgattct cattctgacg
       61 tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca tttcacactc
      121 ctgttgacca cctggtctta actggaattt aaatgcctgt aatcgtttca caggtcaaat
      181 gatactttgg tctgtgaata aagaaaccga tatccggcgt ggaaggcatt gtgttttcct
      241 gatgcatgtt cacctggtct ttgtcaccag ataccgacgc cagatttttt gacgcgacag
      301 aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa ctggttgaaa
      361 tggatggcga accagatcac gtccatttgt taattaacta acctcccaaa ctggcgatat
      421 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag
      481 atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta
      541 gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg
      601 gtcaggtgga aaaccgcacc ttatatcccc gccctgaagg atggggtttt acggcacacc
      661 ggataaaaag cgccatctaa cgatggcgcg tctggaacta ctttttgtta acgtcaaaca
      721 tggtggcatc ggtcgccatg tcatcaataa cttgtttaat attttcgaaa gccattggcg
      781 tactttcgtt attcaggtct ttaccttcgc cctgacgaac gactttgata accggtttat
      841 tagtcgctgc atcaatcagc tcaccttcaa aatagaggcg agtatccatg gtacggtggc
      901 ctgtagccat ttgcgtcccc gcaaccacta atgcaacagg aaccacttca tagaattgca
      961 gcccttcttt gctggtatct acaccggtaa tggccccacg gaaaatcaga ctacgcggcc
     1021 cagcggtggt aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat
     1081 agttcaaaat tttatccaga actttctgcc ctacctgggt agaaggtttt ggcaccggat
     1141 aataagtgat tgggttccag acgatgctgt catatttgct ttgatcaaaa ctcgggtcta
     1201 cccaacgtaa aacaggttta cctgttgctg aggttgtttc ttttaaatca gagtaattgt
     1261 ttaaaaaacc agaatattta tctggctggg tgattttaga ggcacaacca gacagagcca
     1321 acaagccgca taaagctgca actttcgcaa atgatgtggt acgcatgaat agtttcctga
     1381 gatgattata ttacttttta agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa
     1441 agtgttggtg aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa
     1501 ggggctaagt caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga
     1561 ctactgtgat taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag
     1621 taaatcatct cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg
     1681 tgccagcatc gtggcgaaac gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag
     1741 ctcgccgacg tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc
     1801 tccctctttg aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc
     1861 cgccacaatc aggttgtaac caccaggttt agtgcaacgt tgcatattgg caatcagccc
     1921 tgggatggtt ttagcctcaa ggaacatcag taccacagtc gaaagaataa aatcgtactg
     1981 tctatcaaat gtgaggttat tcagatcaac gacccgggtg tgtaaattat ccagattttc
     2041 aatggattta atgcgctcta cattggcgat actcatggca tttttatccc atgcgtcaac
     2101 atcataacca ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag
     2161 cgttttaccc ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa
     2221 ttcatattta tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc
     2281 attaaaacgg cgcgctcggt tctgactaac aattttccct gcatcagaag cgcaaaagta
     2341 cggatgagca gtattgcaat aataaaattg gtaaagataa acagcggtac cgccagcgta
     2401 tggaaaaatc cattttcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat
     2461 acgccgaacg agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc
     2521 agacgtagca taaacagcag ttgcagcagt ccataaccaa aaagcatttt cgccagcgta
     2581 tcaccctcgc cgccgttgac gctcaaccaa gcactacagg ccaccagcgc aggagcgagc
     2641 tgaatgccga gtgatgtccg cagtgccgtg ggtaattctc ccgaactgcg cagacgctgc
     2701 aagatcacag gttcaaggct tagccatgag aaaacgcctg cgcctaaaaa caccagaccg
     2761 gcgtcggtgt agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc
     2821 ggcagataca gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca
     2881 gtttgccagg cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc
     2941 agtgggcgaa accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa
     3001 ctcacaaaac tgctcatcac tggatggcgc acttctgcca gcacgctatg cggaaagcgt
     3061 atgagtcggg taataaatgc gctagttaac aatccccaga tgatcatcgc cagaatcacc
     3121 agcccatccc ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat
     3181 cccatcccta tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta
     3241 tcgctctgca tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt
     3301 atggaatttt ctcgagcata gccagagccg cagaatttgc tacggttata gtaagtcacc
     3361 tgtgggaaaa cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc
     3421 aggaagatgg cactaaaaaa aatgtgttat tacggcagat tatcgccatt agcgatttta
     3481 acgatgtgat agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg
     3541 aaggaaattg ctggatttac gaccagaacg ctattgcgtt cggcggagcg gtgatttccg
     3601 gtaatacgcg catcaccggc accagcgtgc tgtggggaga ggtttacgca acggataatg
     3661 tctggatcga taatagtgaa atcagccagg gtgcatatat aagtgacagc gtcaccattc
     3721 atgactcact ggtttgcggg caatgtagaa tatttggtca cgccttaatt aatcaacatt
     3781 ctatgatcgt cgcagcacaa ggcttaacgc ccgaccatca actcctgctc caaatttatg
     3841 atcgcgcaag ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg
     3901 tcgtccggta tgcttttatt gaacatcgcg ccgaagtttt tgattttgcc agtgttgaag
     3961 gtaatgaaga gaacaacgtc tggctatgcg attgcgctaa agtctacggt cacgcgcagg
     4021 tgaaagctgg catagaagaa gatgccattc cgacgattca ttacagttcg caggtggcgg
     4081 aatatgccat tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg
     4141 ccgtggtacg tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc
     4201 gtatttcagg tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccgtcgttg
     4261 aagcctttga tggcgacacg gttcatgtcc gtggtccaaa agtcattaac ggcgaggagc
     4321 gcattacgcg aacgccgctg gcagggttat tatgaatcga taatacgcgc gtataagttc
     4381 acatcatcat aggcatcatt caggaactca gcctgtttca ggcaaccttc aaggataaaa
     4441 ccattgcgca aagcgacctg gttgctttgc ggattgtcca cccgacattt gatcacgaag
     4501 cgtctaagtt caccagactg agcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg
     4561 atcccctgcc cctgatgaga ttcgtccagc cagtagccga tttcagcagt tttattcagt
     4621 ggttcaatac gattaaacga gataacgccg ataagttcat cttctttgaa aatcatgaac
     4681 attttggcat agccgcgttg atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc
     4741 tcttcactct gaacgaactg cggccagttt agcgactgct gtaaccaggt tttattttta
     4801 cagattaact gataaagagg tgtgacgtga ctttccgcaa cagcatgtaa ttcaagtgat
     4861 tcgcttactt ttatcgtttc agtcatctta tctccagtca atccatgctg ttgttaaaaa
     4921 agccgcctgg ctggcggctt aggaaagttt acacctcttt gacgctctcc tgctgcagga
     4981 ttttgagcat ctcatctttt aactgtagct tttgtttttt catgcggacc acttccgcat
     5041 tgtaccctcg accgtcggaa ccttcctttc tggcaatttc atgatcaagt ttattgtgtt
     5101 tatcgaacaa ggacataaag cgaggatttt cgtttttcag acgggatatt aaatctcggt
     5161 attctggaaa catacgcact ccctgttagt ggctgacaag gtgtatctga aatacacacc
     5221 atcagattac tcatttaact tagggaaaaa tgcgagcggg atcgtgttta accagacggc
     5281 acctcccgaa gaaggtgccg aagaaaaaac gattaactca tcacgcggtc gtcaacatac
     5341 tgacgtttat ccggcgctgg cgggaaatac tgatacagcc atgtttcact gatagcgtcc
     5401 ccctgacaac gtagatacat ccgcatatcg accggatcag tggagtccga agtcggatac
     5461 cagtcaaact gaatacgata accatcgatg ggttcaatat agagaatttc gatttgcttc
     5521 gcttccccac tggaaagcgt aatcaccggc tcaatgcctt ttggcgcggc agctttcaga
     5581 tcaccaccaa cgaaatcgac ggcaaaacga cgcgcccatt tttcgggata gtgttcacct
     5641 ggcgcccaac cttccgggaa accgcccatg ccggtacgcg tcgccataac gcgcgctaat
     5701 gggcaatgaa caggcggttg cgcactccag tacagacgat actggaatgc aaactcatca
     5761 cctgctttta cagctttttc tggctgccag aagcagacaa tgttatccag cgtttcgccc
     5821 gttgttggga tttccatcag gccgatggtg cccttacccc acttgttacg cggttccacc
     5881 cacagacttg ggcgtttgtt ataccagccc ataatgtcct gataatggga gaagtcacga
     5941 tccagttgca ataaaccaaa ccctttcggg ttgttgtcgg tgtaagcatt gaactgcaat
     6001 ttttgcggat tattcagcgg acggcaaatc cactcgccgt tgccccgcca catggacaga
     6061 cgatcagagt catgaatttg cggatgaatt gtatcgcaca tccgacgttc attagtaccg
     6121 cagctgaaca tactggtcat cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac
     6181 aggtgatttt ccacatccat aatcacctga cttttctcac aatggatagt gaacttatag
     6241 gcaccagtaa tgctggcgct atcgagcaac gcataaacgg taaaggtagt tgcccccggt
     6301 tttaccgtat caaaccagaa agcggtaaag tcggggaact cttctttact gtcggtgtaa
     6361 gtgtcgatcg ccaggccgcg ggccgacaaa ccgtattgat atgtatcatc aacggcgcgg
     6421 aaataactcg cgccgagaaa tgatactaca tcacggcgcg ccagttcggg ggctttaaac
     6481 acgcgaaaac cggcaaagcc gagatcgctt tgcccttcta attgtttggt atcaacaccc
     6541 gcatcgttgt atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct
     6601 ggatctacag aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga
     6661 gcgtccagtt gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata
     6721 ctgttataag cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca
     6781 cgccacgctg tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc
     6841 tgggtttgcc cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa
     6901 gcaatgccgc tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta
     6961 cgatccatac ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt
     7021 cagcggcaag aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc
     7081 aattgttgaa ctgagagaat atgcataaat cggcattttt tttatgtcga tgggatgagc
     7141 ctgattcgtt tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca
     7201 aaccacagcg acgtttactg cgttcttccg cttcctggta aagctggaac tcatccaaaa
     7261 ccggacaacc caacgcctgc tcaaatgctt cgcgactggc attaccgtga ctgcgtgtct
     7321 gcgtttcatt acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt
     7381 cataggtgat gggttgcgcc actacccaac cacgttcaat taagggctgt ggatcgtctc
     7441 caggatgaat cgcctgacga tgcgcctcac ccgaaggcgt cagacggtac cggaaccatg
     7501 ccaacccttg ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta
     7561 aatgcatttg gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat
     7621 acagttgtcg ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg
     7681 cggtatgcgt gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc
     7741 tggtctggcg tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg
     7801 gttcaattcc gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg
     7861 tcaggtggtt gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt
     7921 tgtgcaaggc gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt
     7981 ccagggcttc ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat
     8041 attcctctaa cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg
     8101 ccgctttctg gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa
     8161 cgcggaaggg attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta
     8221 ccggcacccc tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa
     8281 acatccgccg aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt
     8341 caacattcag tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt
     8401 tttccagcac agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa
     8461 cttcttgctg gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg
     8521 tgatgctgtt cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaaccg
     8581 cattcactct tgcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta
     8641 tccattttgc tgtaacataa ttgctttaat tgttaataat attttgcaat caagttatca
     8701 taatcaaaca acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa
     8761 aaatagtctg tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc
     8821 acaacaagga actttggggc gcgcggctga aacccttaat ttgagtcaac ctgcgctctc
     8881 taagacattg aatgaactgg agcagctgac tggcgttcgc ttgtttgagc gtggtcgtca
     8941 gggggcgcaa cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga
     9001 cgccatcaac actgccggac agtcgcttca tcgtaaagaa ggtcttaata atgatgtcgt
     9061 cagggttggt gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt
     9121 tcatcagcaa caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct
     9181 ggcgggttta aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact
     9241 gatgaccggg cttaattacg aactgctgtt tcttgaatcg ttgaagctgg ttgtccgccc
     9301 taatcacccg ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt
     9361 tgtatcacca gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg
     9421 ctgtaaaatt ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac
     9481 ggttgaatac gattacgtgt ggtttgtccc ttctggcgct gtaaaagacg acctgcgtca
     9541 tgccacgctg gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact
     9601 gacccgcgta gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa
     9661 atcaatgccg ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc
     9721 gtagcgaaac taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgtgc
     9781 agacggaata catccaccgc ctcagttaat cgtgccgcct gttcttcaag ggacaccgcc
     9841 gccgctgagg cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa
     9901 atcgcctggc taacctgcgt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc
     9961 tgcatgatat gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc
    10021 gttgccacct catccgaccc caggtcaatt aacctgactg attcactgat caagccttca
    10081 atctctttcg ccgcttgggc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg
    10141 gcaaatccac ggccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga
    10201 atattcgtct ggaaagcaat actgttgatg acggcggtga tctcggaaat tttcttcgaa
    10261 cttgtagaga tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg
    10321 gctttaatag aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt
    10381 ttcaccgtcg cagtgagttg ctccatgcta gcggcagttt gttcgatagc cgccgcttgt
    10441 tcttcggtgc gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa
    10501 atctcttccg caccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgcatttgc
    10561 tgtaaatgac gatttaagcg accgatttca ttacgacccg ccggttcatc gttcatcgtc
    10621 agatcgccac tagcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg
    10681 atccgacgta tcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca
    10741 atcatgaaca tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggct
    10801 ctggtgctac gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt
    10861 cggatctgct cactttcatg attaataatc gcttcaaaca tgccattttt ggcatatttc
    10921 aacataggtt gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg
    10981 gcttcatcag caggtgtttt caccggccga ttctgataag cacgataacc ttgctgcgac
    11041 tgtttaatct ccgattcggc ttgcgcaata tttcgcttca ttgcttccat ttccgcaata
    11101 cgactcgcag ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg
    11161 ttcgctaacc cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc
    11221 tgccagagga aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca
    11281 agaatgcagg caaataacgg aaccagtctg atgtgatgca aaaaacctaa tcgctgtgag
    11341 ggagttgtat tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg
    11401 cacttgacgg taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta
    11461 ttgcagagaa aattaatgct attggaatga agagagtaac gaatggctaa actggctaac
    11521 ccgaatgacg aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc
    11581 ctaaggaaac ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg
    11641 ggaatcagaa agacgaaggc cccgaccgat atttctatca accgaggcca acgaatgaac
    11701 ctagcaattc agatcttagc ctcttacccg ccatccggca aggagaaagg ctatgaagca
    11761 caaccctctg gtggtgtgtc tgctcattat ctgcattacg attctgacat tcacactcct
    11821 gacccgacaa acgctctacg aactgcggtt ccgggacggt gataaggagg ttgctgcgct
    11881 catggcctgc acgtccaggt aagggcaagc gcggggattt tccccgcgca tctgactagc
    11941 agattcattt tctcatggca ccccttttac acttcagtta gtgcatgttt ttttcgaggc
    12001 actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg agatcccccc
    12061 tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa gcgttcccat
    12121 aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat cttatgttcg
    12181 atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag atcgggtgaa
    12241 ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat ttgtcataca
    12301 aataaatata ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg
    12361 ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta
    12421 gaagtgtgtt atccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga
    12481 tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt
    12541 cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc
    12601 ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca
    12661 aatgtcccaa atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg
    12721 ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac
    12781 aggaaacatg aaccatgttg atgagtggcc gatcgctacg tgggaagaaa ccacgaaact
    12841 ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta
    12901 accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa
    12961 cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca
    13021 ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa ggatcaattt
    13081 tcatccgaat gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt
    13141 cgtatcggtc gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc
    13201 gctattaatg atctcacttc cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac
    13261 tacggaccat tcccctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa
    13321 agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggtgca
    13381 gaaatcattg tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt
    13441 gatgctggtg cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt
    13501 tataacgtca atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc
    13561 accactaact gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc
    13621 ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt
    13681 ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg
    13741 gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg
    13801 caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct cggaaaaaaa
    13861 gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt
    13921 ggttattccg atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg
    13981 tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc
    14041 gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc
    14101 gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac
    14161 tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg
    14221 ccaataccgg atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg
    14281 ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt
    14341 tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct
    14401 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
    14461 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct
    14521 accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
    14581 tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca
    14641 gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca
    14701 taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc actattaatg
    14761 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
    14821 gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc
    14881 atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta
    14941 cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc
    15001 ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca
    15061 ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg
    15121 aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac
    15181 aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc
    15241 cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc
    15301 tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc
    15361 ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct
    15421 ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt
    15481 atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc
    15541 caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac
    15601 tcctgtgatt tatatgttta ttttcggtga atgcattgtt aattaacatc aatcatcaga
    15661 gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
    15721 ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac
    15781 gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt
    15841 cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
    15901 cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag
    15961 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
    16021 tgcattattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
    16081 attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg
    16141 aacaacgata gccgtatgaa ctcgttctac ggcctgtttc aatagcgcga tgctaaagcg
    16201 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
    16261 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
    16321 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
    16381 accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat
    16441 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
    16501 ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg
    16561 cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac
    16621 tgcgtgagat aatagtgaga agcggagcac tctgaaagga ggaagtcgta tgtccttaac
    16681 cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac attattcatc
    16741 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat
    16801 taataaaaaa cccggcgcta aatgcttacc gggttttttc tttatcaggc aaatagcagg
    16861 gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
    16921 taccaagttg ttgagcgaag taactaacgc gcaactcttc tatcatccaa cggatctctt
    16981 tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct
    17041 gctggacgtt ttcgactttc agcatctgag cacggtcgcg atgtggatca accgccagtt
    17101 tttccagccg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc
    17161 cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa
    17221 gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacactg
    17281 ccgtaagaat ttgctcgacc tgcttcgcaa tatccaccac cgtgtcattc agttcggcgc
    17341 gcactttttc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
    17401 cgatcaattg atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
    17461 acgggttaaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt aaatatttga
    17521 ttggcgatgg aatattcagc agcagtagac ggcgaagacc gttccacatt gcctgctttt
    17581 gctccagcgg gttatcaaac agtttgatcg ccacactgtc gcgctcatcc accagcgccg
    17641 gccacgcctt cactttgtag ttgccacgct tctgttcgta gctttccggc agctgaccaa
    17701 aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgca gatagcgttt
    17761 cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct tcttttagct
    17821 tcttgttttt gtcatccacc acgcggaagg taattttcag gtgatcaggc acctgatccc
    17881 agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc
    17941 tgtcgagcaa cggtaactcc agcggtttga cgcggcctaa aaacgcttcg gcatagtttg
    18001 gcgcgggtac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca
    18061 gttcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc tggttaagta
    18121 acggcagcgg aatatgtacg gtcacaccgt cagcatccgc gccgggctca aactgatagc
    18181 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
    18241 tttctgcgcc ctctttgatc aacatgcttt tttcaaagtt gagcaaatca ggggtttcgc
    18301 ggctgacttt tttccaccag ctgtcgaagt gacgagcgga gattacatcg tggctgatgc
    18361 gctggtcgta gaactcaaac aacgtttcgt catcaaccag aatatcgcgg cgacgtgatt
    18421 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
    18481 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacgg cataacgccg
    18541 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
    18601 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgaggttcg ctgtaggtgc
    18661 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
    18721 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
    18781 tgaataaacc agaaccggga aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
    18841 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
    18901 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctggc
    18961 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcggtac
    19021 gacacaggcg acggaaggcg ttggaagaaa gcgccttttg ctgctcgcca agataattcc
    19081 acagattcac aaacgcgagg aagtcagact ctttgtcgtg gaagcgacga tgtttttcgt
    19141 ccgatgcctg ctgtttatcc atcggacgct cgcgcggatc ctgaatggaa agcgcggacg
    19201 tgataatcat cgcctcacgc acgcagccat gtttttgcgc ttccagcacc atacgcgcca
    19261 gacgtggatc gacaggcaac tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
    19321 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
    19381 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
    19441 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
    19501 ccgggcgcga aaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
    19561 gcccacagcg gcctttacgc tgattggcag acgcctgcga aatgggttca atcggcaaac
    19621 gctgcacttt ggtgcgatag ctgtagcggc tgatacgtgc tgtaccgggg tcgataacgt
    19681 atttaatccc cggtacggtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc
    19741 gtccgctgtg cgactggaac acgcggttct gttcgctgtt cgaaagccgc gcataaagcg
    19801 gcaagatttc ggtatgacgc agatttagct tgttcagcgc atcggcggta tcgcgaattt
    19861 cccgctcacc actcataaag atcagaatgt cgccagggct ttcctgactc agttcgtcta
    19921 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc gtcggcttct tcaacaatcg
    19981 gtcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
    20041 taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt
    20101 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
    20161 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
    20221 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca ctaacgtgat
    20281 cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa
    20341 tacggttcgc caccgttctt gccgccaggc gacgcggctg ggtatggccg atcagccctt
    20401 taatcccgcg ccccagctcc atacagattt tcggtaactg agtcgtttta ccagaacccg
    20461 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaatg tcctgttttt
    20521 tctgactaac cggtaaattg tcaggataag taatttccgg tcgtgccgct tcacgcagca
    20581 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
    20641 catcaggatt tttaaccttc ttaacaccgt gcagacgccg agaaaaacgc agtctgtcgc
    20701 gcagcatcag cgaatccagc cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg
    20761 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
    20821 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
    20881 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccca
    20941 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
    21001 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
    21061 cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat
    21121 ggcgaactgg ttggtgcact gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
    21181 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
    21241 gcggcaccga tgtataactt caacatctca actcagttga aaaactattt tgacctggtt
    21301 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
    21361 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg
    21421 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
    21481 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
    21541 gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt
    21601 cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc tttgtgatct
    21661 tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc atcaacggtt
    21721 ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg cgataatccc
    21781 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
    21841 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acagaaatag
    21901 agtcgatctt ttgtcgctcg tccgcgaggg aattcaaagg tgacgtccag aaccgcattc
    21961 tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtcat tttgctcacc
    22021 ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg gcagggcaat
    22081 gtcagccagt aaacggcggg cggtagtttc ccctgactat ctttccctgt tgttatcgcg
    22141 ccaagcccac cgtacccacg gccaattatg agtagcgata atactggcca acataaccag
    22201 actgaccacc ataactgaat catcatcatt agctccatca atacaatgca cgagcccgcg
    22261 cctacgacat agggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
    22321 cgacggtcga cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca
    22381 tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa aaaccatccg
    22441 ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca aagtagccag
    22501 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc
    22561 gaaaatagcc atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
    22621 tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg
    22681 cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc
    22741 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga
    22801 ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag agatccatag
    22861 gtgaagaaga aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
    22921 cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat
    22981 ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga
    23041 acgcacgcgc atcacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag
    23101 ctccagttgt gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc
    23161 attggcaaga cctgcgaggg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    23221 gccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
    23281 cgcatcgccc tgttcaaaac gcacctgccc agacattccc cgttgggcaa tcatctcttg
    23341 cccctgtgca acatttaact cactgtaatc acgtaacaaa atatcgcata cggcaggttc
    23401 attctccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggat
    23461 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagcattt gtaaatgggt
    23521 tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt cgactaaacg
    23581 cccgaatgcg ttactacctt gtggttgatt ttgatagaca taatccagcg aactgcctga
    23641 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat
    23701 cgccatacgc ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccaccaga
    23761 aagtagccgc catcgatccg ctgatggtcc tgtgcagtct tcatgctgat aatcaaattt
    23821 ttgcgatttg ttagcatata aatgactaat aaagctttgc attttttcaa atgccagcgc
    23881 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttgag
    23941 agggctacgt aaacgctgat aaaaatcgat ctgcggttgg cgatgcacca catagtcatc
    24001 gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc
    24061 agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc
    24121 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    24181 attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
    24241 cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc
    24301 ataatcatga acccatgtgg caaccagcac tgcgccgacg ctttgcgcga tcacaaccac
    24361 ctcttccagt ccaacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcccg
    24421 cactgaacgc gcaagagatg ggctataacc acgcggcccc gaactttttc catgccctcg
    24481 ggcatcccag gcataaaaag cagtatctgg catcgccagt tcatcaacga gatgttgtag
    24541 acgaccagaa tgttcatggc cacgatgaaa taagacgatg acctttttcg ccccgggctg
    24601 taaagcgggc cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc
    24661 cccagagatg cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgtat
    24721 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
    24781 atgtcctggt aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat
    24841 cactacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtga
    24901 tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgatgac
    24961 gccccccacc agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct
    25021 gccgcaggat ttcccccata aatactgtgc aatatcgttt gactcggtta atgccaaaag
    25081 aaataacacc agtaacgccc cgccctggat attcatggat ggcaacatta ataaccaggc
    25141 ggcatgactc aaggcgaaaa cggtcattat ccagccccaa aaaatagcgc tcacggtatg
    25201 cagaaaaccg gagggttcac ccacaaaaac tcgccaggtg gctaatatca gaaacccaga
    25261 caaaggtata aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag
    25321 aggaataacc caatataacc accgcggaaa atgtatggat ataagcgtac aatattcttt
    25381 caatgccaga aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat
    25441 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacctcacc
    25501 cccttttctc tccggtaacc gccatagcag aaaacggttt atcagcgttg ctaaaattaa
    25561 caacgcgaaa agtgttgcca gagatttttc cagcattatc tttcagcact cataaaaagc
    25621 acactccgac aacgattaat cgcagtccag agaagcagaa ttgaagcaat gctccataaa
    25681 agattattcc attgcatcca ctgtggataa atggcaacgg ccagccccca caacccaaat
    25741 attaacgcac gatcgctttt gccgaatgga ccggcataac tgcgaatacc gttaattgtc
    25801 tgcgcgagta aaccacaaaa ctcagtcaat atggtgcaaa ataacatgag tattacgagt
    25861 gatgcgttac tttccggtaa aaataaaaac ggtaaataga gcgcaatatc ggaaataaca
    25921 tcaccagtct cattcaaaat tgccccaaac cgtgtttgct gattgcactc acgcgccaac
    25981 atgccatcca gcgcattgag cgccatacgg ataaaaagca cgatgggcaa tagcataaag
    26041 aggatgggtt gtgccaccaa catcaacagc aatccggtaa gaagagaaag cgccagtgca
    26101 gcaagagtga tgtgattcgc tgtaacgtgg tgcttgtaaa gccaaaacat cgtcggcctt
    26161 aacagcgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc
    26221 ggtttgcgct atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta
    26281 aaaattcaga atatatcaag gtattgggga ctcactgtca ggaataatta tccccaatta
    26341 atcaaagggt taagaaatat tacgttgtgg atcaatacaa ctcatcaagt tttctcatcg
    26401 cttgttcccg tattgcgcgc gtcttaccgt taagcgccag ctggcggact aaatccagtt
    26461 cagggttaac aagctctaac gccagcaaag gatcacgttt cagcctcgcc tttaatacat
    26521 caactggaaa acggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg
    26581 ccagtactgc aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat
    26641 tgcttttcgc cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt
    26701 gtgctaacgc atcggggtcg agtgcataat agacgcgtaa tgcttcatca acctgcgcac
    26761 gggagctgtt ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtatttt
    26821 gctgtaattc cttgaggaca acagcgggta aattatccca actaactaat gcactctcta
    26881 aaaagtccca gtcttccaaa cgtatcaggg ctgtggcatt taatagcatc tgatcatcaa
    26941 cttcaccgtg gctgataagc tcagacaaaa tcatatcagc gctggtttca tacgcagctg
    27001 aatctgcttt cggattattt ttccgatgcg ataaagcacg ttgccaaatt ttttgtttca
    27061 tcgacgtgct cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac
    27121 gtgtcaataa ccgttgtaga gcagcgtcag agtgaattac ctgcatatcc cgacagcaaa
    27181 agtaggcagg ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca
    27241 atgctaaaaa taacacacgt attagatcat cgtcattctc gaatttttgg tataaatagg
    27301 ctgcaacttt gtcactctct tgtggcgtca tccgcaataa ttgaccatgg tgtttcatct
    27361 ctgccagctt gaacgctaag cttgtgacaa cattgtttga tgaatcgttc gccaactgca
    27421 ttgcctgatt gtaggtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac
    27481 tgcggatata gccatcaata atggattcag gcaataacca cgcatcttta taaagctcct
    27541 gaatgatcat atcccaatct cttttcgcca gagtcattac gatatccaga gggagatttt
    27601 cacgtagcgc aagattcaac ggaccgacac catcaggtag acgactcatt cgcgtcatta
    27661 tttcgacaga gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt
    27721 tctgatcggt caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg
    27781 tttgttcatc attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc
    27841 ttgggagagt tcggattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag
    27901 caacgttgcc ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttagggc
    27961 gaaacagcgc ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc
    28021 gcttaaaaat ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag
    28081 gatttattat catcagcgct tttttgacat tttcagtgtc atttttgtca ctcgcacgga
    28141 ccatgaattc actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata
    28201 tcccacgcgc attaatactc cccttaatcg tagacggatt atcgtaatat gagtcgataa
    28261 ataccagtcc acttgcattt atcgaaccgg taattaacag tggtgaatcc gtaactatat
    28321 tcgttgccgt cacgttaccg agaataaccc atggattaga aagagagaaa gtggaaatat
    28381 tacctttgac tgtcaaatta ccaagaacaa tcaatttttg attactctct atattacctt
    28441 ctaccactaa atcgccacga tgcactttcc agtccgatgc agataaaccg aaggggacaa
    28501 gattcacgct actgttgata gcttgttcaa cttgttgttt atcttcagcg gtagccgcag
    28561 aaaaaaatgg ttcaatatca actgtatttg caacacatcg aagttccgcc tgtactggaa
    28621 gactcagcat aaacagggga agtaaaccta tccagccgca aatcctggcc ttaaacatat
    28681 gcactccatg ccattaaaaa agaaacaaaa gggaaatgca tctttccctt ttgtatattc
    28741 gttattctcg cgaaatacca tccaacgtag acaacacttc ctcagaaaga tgcaattttt
    28801 cagcagccat attctcccgt aaatgcgcaa cagaagacgt ccctgggatc agcaaaatat
    28861 tcggtgaacg ttgtaacagc cacgccagcg ccacctgcat gggtgttgca cccaggctcg
    28921 cagcaacatc ggaaagtgtg gacgattgca gcggtgtaaa gcccccgagc gggaagaacg
    28981 gcacgtaggc aatgccatcg tgggccaaag catcaatcat tgcatcatca gcacggtgcg
    29041 cgatgttgta ttcgttttgc acacagacaa tttcggcaat cttgcgcgcc tctgcaacct
    29101 gcgtcggtgt gacgttgctc aggccaatat gttttaccag gccttgttgt tgcatctctg
    29161 ccagcacggt caggctggcc tcaatcgatc cttccgctgg gccatgacca tcccccatca
    29221 taatgcgcaa gttaaccaca tccagtacgt ccaggccgag attacgtaga ttatcgtgca
    29281 ccgctttttg caactctgcc ggagaaaagg cgggcaacca ggaagcgtcc tctccacgcc
    29341 gcgcaccaat tttagtgaca attgtcaggt cgtcagagta aggataaagc gcttcgcgga
    29401 taatctgatt ggtgacgtgc ggaccataaa agtcgctggt atcaatgtga ttgacgccca
    29461 atgccagcgc ctcgcgcagc acagttatag cgacgtggcg atctcttggg ggaccaaaaa
    29521 ccccaggacc tgccagttgc atcgcgccat aaccaagacg gttaacggat tttgtaccga
    29581 gagtaaatgt attgctgctc atcgcaccct gccctgtaat aaaacggatt aagaaaaatc
    29641 agtatagaaa cgaaaagaga taaataatgc gagggcgtaa acaagattac tggatgagtt
    29701 gctctattgt cctgaaaaac acaaaacaaa acgcgtaaag cgccgcatcc ggcaataaaa
    29761 aaaccgcaac ctgtatcagc gttgcggttt aggtatcaca tgctaaaaat tagaatgttc
    29821 agtatctgga cgttactgtt tcggtcgcag attctcatca aataccaggc gctgcctgcc
    29881 aggttcaact tcccgcaatg gttcgacttg atgcaacgtc tgcttacagc gtgtcaccag
    29941 cacctggtac tcatgcgtac cctgcttttt ccatgccaac tcctgctcac tgagttcacg
    30001 aatcgctttc gccgggctgc cgacaatcag gtaattggca ggcatttctg ctttggcttt
    30061 cacaaatgcg gatgcgccaa caatgctgtt ctcgccaatc accgcgccgt ccatcactac
    30121 cgcgttcatt cccactaatg cattgcggcg gataatgcaa ccatgaagga tagcgctatg
    30181 accaatatgt ccatcttctt ccacgacagt atcctgctcg ggaaaaccgt gcataacgca
    30241 attatcctga atgttcgcgc catctttcac cacgatacga ccaaaatcgc cacgcaggct
    30301 ggcatttggc ccaacgtaaa cgcccttgcc tagaataaca tcgccgatca ataccgctgt
    30361 cggatggaca aaactctctt ctggcacaac cggagtcaga ccatctatct ggtaaattgg
    30421 catatcgcct cctgttcaat attcaaaccg ccaaaacgtt gaaaatacag gcttcccggc
    30481 gcaggcagtt caccgaccga ggtttcacct ttttcactaa cgaacgctaa agcagcaggc
    30541 gctacgcgct gataaatgtt gatacacagc tgacgcgccg tatgccctgc ccagtgcgcc
    30601 ggaagcaact cctctggcaa caatgggtct ttaaggacga cacggcgata aaaatggatc
    30661 agtaaaagct gaatatgaaa tgcacgctcc ggggttaact cgtctgctgc ctcttttaac
    30721 agcggcacca gcgggcggaa tgactgaata aaggtttcgt acatggcatt ttgttcagtt
    30781 aaatgccagc actcttccac tctggcacgc agtgctgcac gagaaagcgc cagtggtatt
    30841 tgcgcttcaa aacaaatcac gttatccgcc acccccgctt catgcaaaag tgtctgtaca
    30901 tccgccagtt tttgcgacgg cgatgccatc aggctgggtg ccagtgcgcc aaaaccttgc
    30961 cagatcaact gctttttgac atcagccagc gttgatttat ctaaaccttc cgagagcaac
    31021 aggagccatt taccatccca tgcaggttgc tctgcgcgat aaattttact ttctgcccgt
    31081 cgcgtcaggc gcaagccttt atcactgagg ctatagaaac tgcgtcgccc gatgcgggaa
    31141 acatccagcc agccttcttt attaagacga aacaaagcgg tgcgcacgaa acgctcacca
    31201 aatcccagcc cttccagcaa agcagccaga ctacccagcc agatttcacc accacgatgg
    31261 gaaagcgaat caccatacag agaggagatc aaagatgtgc cactgaccgg aacagcgttt
    31321 acagcatgtt ggataaaagt aacaagttta ctcattcgat tcagtctgtt gttattgttg
    31381 tgatttatga atcatagcac aggtcgtatc gacttctggc cctggcgctt tacctcacca
    31441 gggccagacg acacgtcagg caccaacaat attgcgcaga tcaaacaccc gacacgcctt
    31501 gccttctgaa cgcgggatac tgccacagtt aacgatcatc acatcggtag agatccccac
    31561 catcgactta atccgatggc gcagctggtg gcacacctgg caacgctgct catgcgtcaa
    31621 tgtcaggctg ctttctttca actccacttt cacagaaagt gaatcaagat gcccccggcg
    31681 gttcacctcc agttggtaat ggggcgataa atgttcgaat ttgacaatct cttcttccag
    31741 ttgtgacggg aagacattta caccgcgaat gatcaacatg tcatcgctgc gtccgctgat
    31801 gcgatccatg cggcgcatag tccgcgccgt tcctggtaac agtcgggtta aatcacgcgt
    31861 gcggtaacga atgaccggca atgcttcttt ggtcagcgtg gtgaataaca gttcgccatg
    31921 ttcaccatcg gcaagcggtg tgccgtcatg aggattaaca atttcaggat agaaatgatc
    31981 ttcccaaatg gttgggccgt cggtagtttc cagacactcc atcgccaccc ccggccccat
    32041 cacttctgac aggccataaa tatccagtgc ggtgatcccc agacgccgtt caatctcttt
    32101 acgcatggcc tgtgtccacg gctcagcacc aaacacgcca acccgcagcg agcaaccgct
    32161 ggcatcaccg cccaactgcc gctccagctc ttcaatcagg ttaaggcaat aagatggcgt
    32221 aaccatgatc atatctggtt gaaaatcgcg gatcagttgc gcttgttttt ccgtctgccc
    32281 accagacatc gggatcaccg tagcgcccag acgttcggca ccatagtgcg cgcccagccc
    32341 accagtaaac aggccgtaac cataggcaac atgaattttg tctttgggcg tgccacctgc
    32401 cgcacgcaag gaacgcgcta caatattggc ccagttatca atatcgtttt gcgtatagcc
    32461 gacaactgtc ggtttacctg tggttcccga agaagcatga atgcgcacca cttgttccat
    32521 cggcacggca aaggtgtcga agggatagtt gtcgcgcaga tcctgtttgg tggtacaggg
    32581 aaatttacgc agatctgaaa gttccctgaa atcatcagga tgtacgcctg ctgcgtcaaa
    32641 tttgcgccga tacatcggga cattttcata cgcgtgtttg agcgtccatt tcagacgttg
    32701 tgtttgcaac gcctgtaact catcaacaga cgcggtttca atcgggtcaa gctttgtatt
    32761 ggttatcatt gtagggtact cgcaggttga tatgctcaaa cacgctccag aatcatggcg
    32821 atgccctgac cgacaccgat gcacatggtg cacaatgcgt aacgaccgtt acgccgatgc
    32881 agctcatggc tggcagccag tgccaggcgg gcaccactca ttcccaacgg atggcctaag
    32941 gcgatagcgc ctccgttggg gttaacatgt ggggcatcat caggcagccc caattcgcgt
    33001 agtacaccca acgcctgggc cgcgaacgct tcgttcagtt caatcacgtc catatcgtga
    33061 atactcagcc ccgcgcgttc cagcacccgg cgagttgcag gcaccggacc aagccccatc
    33121 aggcgcggtt ccaccccggc ggttgccatg gctacgattc gcgcccgcgg tgtcagtcct
    33181 tgcgctgctg ccatctgttc actggcaata atcaacgcag cggctccgtc attcaccccg
    33241 gaggcattgc ctgcggtaat caccccattg gcacgaaatg gtgcttttaa cccacgtaac
    33301 tgttccagcg tcgtttccgg gcgcagatgc tcatcatgtt gtatttctgt tacaacgcct
    33361 ttcttgtttt tcaacacaac cggaacaatc tcctcagcca gaatgcctga ggattgcgct
    33421 tttgccgtac gttgctgact gcgtagcgca aaactatctt gatcttctcg cgatattttt
    33481 aacagttcag ctacattctc tgccgtttcc ggcatgctgt cagttccaaa ttgttgagcc
    33541 atgagcgggt tcacaaatcg ccagccaata gtggtatcaa acatctcagc ctgacgagaa
    33601 aatgcactgg ttgccttgcc cataacaaac ggtgcccgag acattgactc cacgccacca
    33661 gcgatcagca aatcgccatc gcccgcttta atcgcccgtg cggcgaaccc cagtgcgtcc
    33721 agcccggaac cacacaagcg gttaatggtt gtgccggaaa cactctgcgg cagccccgcc
    33781 agtaacgtcg ccatccgggc tacgttacgg ttgtcttctc ccgcctgatt agcacagccg
    33841 aggatcacat catcgataca ctccgcatcg agacgcgggt ttcgcaccag cagctcccgc
    33901 aaagggatag cagccagatc gtcagcccga acacctgata atgccccacc atagcggcca
    33961 attggcgtac gaattccgtc acagataaag gcttcacgca tcaggcttct cctgtaatgg
    34021 tgccgccgat gcggtgagat ttaccgcgaa acagcgcaac cgttttttgt tgttggttaa
    34081 caatttcgat gtcgtaaaca ccggtttgct tgccctgatg acgtacctgc gcagtcgcag
    34141 ttaaggtgtc tccggcaaag cctggacgca aaaaatcaat cgtgcaggca gaagcgacgg
    34201 ctgccagccc ctggctattg caggcgtagg caaaggcagt atcagccagt gaaaatagct
    34261 gcccgccgtg gcaactttga tgaccgttaa gcatttgtgc agtgacggtc atggtcacta
    34321 cagcaaagcc ttcatccatt gagataatgt cgatgccaag cgctttggcg caggcatcgt
    34381 tctcatacat tgcatgggca ttttgccagg ccttatgact cataaccgct ctccagaagc
    34441 gctcgttggc gcagcaatga acatgggcga tagcgttctt cgccatagtg atgttgtaga
    34501 ttttccagga gccttaatat tcgctgccag ccaagttgcg ctccccaggc aagcgggcca
    34561 tatggataat tcaccccaag acgcatggcg gtatcgatat cctgttcaga ggccacgcct
    34621 ttttgcagcg catcaagggc ttcattgatg atcattgcta ccgttcgcca aatcagcatt
    34681 cctgggtaat ctgcaatttg cagcactgtt ttgccctgct gttgcaggta ataaatggcc
    34741 ttgcgggtcg ctgagtccgg gttcaccgct gccgcagcaa tggtcaccac cttgcccgcc
    34801 attttgtcga ccactaccac cgggcgtgcc agtcgtattg ccagcgcctg tgccgtctcg
    34861 ccttgtgtct caatcaataa aacatcgtca atttccgtga cgccgtcact tttcttttct
    34921 actttcattg ggctaaaact gtcgcttacc gcttccaggc caacaactgc ctcacgttcc
    34981 gcgcgccagt cgtacacgcc cagcccactt ttcttgccca accgtccacc aatcaccagt
    35041 tcctgttgca ccagcgaagg taaaaaacga cgctcctgcc agaaagcgtt aaacaccgaa
    35101 caggtgacag caaaattgac gtcctgacca atcagatcgg ttaattccag cggccccatc
    35161 gggaaaccag cgccatcgcg aagtgcagcg tcaatcactt ctggtgcagc aacctgctct
    35221 tccagtgccc gccaggcctc ggaataataa ggacgcgcca cacggttaac gataaatcca
    35281 ggggtcgaat gacagcgcac aggctgctta ccccaactca acgttagttc acacaactgc
    35341 tcaacaactt ccgctgccgt tgccagccca ctgaccacct ccaccaactt catcaccggt
    35401 gccgggttaa aaaaatgcag ccccgcaaca cgttcaggat tttttacctc cgcagcaatc
    35461 gcggttatag agattgacga agtgttagtg gtcaatagcg tttgtggcgg gcaaacttcc
    35521 gccagctgtg caaagagcgc ttttttgact tccagacgtt cagacgccgc ttcaatgacc
    35581 aggtccgcag ctgccagcgc gtgaatatcg gtcaccggga tcaggcgttt caatgtgcgt
    35641 tcacaggttt cagcagtcag ttttccccgc gtcacgcgtg aatttagccg cgcgtgtatc
    35701 ccgtcgattg cgcgggtcag cgcttcagca gaaatgtcat acagtaaaac ctgatgtcca
    35761 tgactggcgg caacttcagc aatgccagcc cccatggtgc cgctcccaat cactgccaca
    35821 gtttgcacat ttatcatcat gctatttccc cgtgaactgc ggtgagcgtt tagccaggaa
    35881 cgcactgaca ccttcacgat aatctgcgct ccgtccggca agtcgctgat agtcgcgttc
    35941 cagatccagt tgcgtatcga gcgtattggt ttcagcgcta tttatcgctt gcttgataag
    36001 tccaagacca aatgtcggtt gtgtcgccag atgccgtgcc agctgttgcg cggtatctgc
    36061 cagcgtttca tcatcaacaa cctgccagat catcccccat tcgtgcgcct gttcagcact
    36121 cagttgattc cccagcagtg ccagccccat ggcgcgcgct cgcccagcaa cgcgtggcag
    36181 taaccaggtt ccaccgcaat cgggtactaa acctaactta ctaaacgcca tgacgaattt
    36241 tgctgaacgg gcagcaataa cgatgtcgca tcccagtgcc agtgttgcgc cagcccctgc
    36301 cgccacgcca ttgactgcac agatcaccgg ttttggcagt tttgccaggc gacgtaccag
    36361 tgggttatag aaacgttcaa ctgacatccc taaatccggt gcggggcctg tgggatcaac
    36421 gttacgatcg ttaagatcct gaccagcaca aaatccgcgc ccggcacctg taagtaacag
    36481 gcaacggata gtgtcgtcgc gctcgacctg tttcaggcac tctgccagtt gtgcgtgcat
    36541 ctcatcatta aaactgttca ggcgttccgg gcggttgagc gttagtgtca tcacgccctt
    36601 ttctacatga ctgaggatga attccatcat tagcgtcctt taaagtcggg cgtgcgtttt
    36661 tgcaagaaag cggagatgcc ttcatgacga tcttctgttg ccgccagcaa ggtgaataac
    36721 tgtcgctcct gggcaagtcc ggcttgcaaa gccacttcct gcgactggcg cagcgcttgc
    36781 tttgccgctt gtaaggccag cggcgagtga cgtgccattt tcgatgccag ctgtaaggcg
    36841 tattcgaggg ttaaatcgct ggggaagacg tcgctaacca gcccggcctg ctgtgcttgc
    36901 tgagcggtga tactttctcc gctcagcacc attttgctgg ctaacgattt accgacacta
    36961 cggattaaac gttgcgttcc tcctgcgcct ggcatgatgc cgagagtgat ttccggcaaa
    37021 ccaaaacgcg cgttctctcc ggcaaccacc acatcgcaca acaatgccag ttcgcaaccc
    37081 gcaccaagcg cgtaaccgtt gacggctgcg atgagaggtt tgttgaaggc ctgcaatcgc
    37141 gcccatagct gcgggcgtgt atcgtttaag gtggccgcga gatctttttc tgccatttcg
    37201 ttgagatcgg ccccagcggc aaaaaagcgt gcattaccgg taatcacaca gaccgaaatg
    37261 ctgctatcgg tagccgcagc ttccagttca tttaccagtt gcgtcagaag ggcattattt
    37321 agcgcattac gtgcggcggg acggttaagg gtcagcaaca atacttgttg ctgacggctg
    37381 acgatcagtt cgctcatgcc atccccttcg cgtcaaagtc aaccaccaca tcgctggtca
    37441 gcggcagtgc ctggcaactc aacacatagc ctgcggccag ttcatccggt tccagactgt
    37501 aattggtttc catcgccact ttgccacgca gcactttgca tttgcaggtc gcacagacgc
    37561 cgcctttgca ggcatagggc agatcagccc cctggcgcaa tgccgcatcg agaatgcttt
    37621 catcgtcggc attaagcacg atttcccgat cccgcccatc ctgacgtaca gtcacttttt
    37681 gtccgtcact ttgcacgtta acgctacgtt tgacgcgcgt gccaggcgta ttaaaccgct
    37741 ccagatgaat ggttttatct ggcattccca gtgcttttaa ggcggcttcc gtctcatcca
    37801 tcatcgccgc cggaccacaa ataaatgcct catcataaag acgaaaatta atgagcgagg
    37861 ccccaagtga ctgtaatttt tcaccgtcaa tacgcccgtg aagcagatcg ctgtcgaggg
    37921 tttcctgact gaaaatgcac aacaactgta aacgctgagg atatttgtct ttcaggtctg
    37981 ccagtgcctg gcgaaacatc atgctctggc tggtacggtt accgtagatc agggtgaact
    38041 gactttcagg ctcggtttgt aaagtggtgg cgataatcgc cagcattggc gtaatacctg
    38101 atcctgctgc aatagccaga tagcggccct ggcgttcggc ctgtggctga tagccgaaat
    38161 gcccctgcgg caccatgacc tccagcgtca taccctggcg gatgtgttcg cgggcatagc
    38221 gggagaaacg tccgccttca atggctttca ccgccacact aatttcgcca ggcaggtagc
    38281 tgcggcaaat ggagtaacaa cggcgtaatt cttcaccatc aagactggct tttaaggtca
    38341 aatgttgacc ggggcgaaag cgatacgcct cctgcaaagg ctggggcacc gcaaaggtaa
    38401 tggtcaccgc atcacgggtt tccggctcga cttttgccac tgttaaggaa tgaaacgttg
    38461 tcatggcatc ctcaaataca tttgaaataa tcgaaaggtt cgcggcaact atcgcagcgg
    38521 tacaatgctt tgcaggccgt ggaaccaaat tcactgataa gtgtggtatg gacgctggcg
    38581 cagcgcgggc aacgtacttc tggcggcaaa tgggcatggc aactgtgtcc ggcgggcggg
    38641 ctaatgccat actgtcgcag acgctcacgg gcatcggggg tcatccagtc ggtggtccat
    38701 gccgggtcga gttgcagcac aacctgaacg ggggtaaacc cgtgggttgt cattgcctca
    38761 cgtatcgcgc caatcaaatg ttccgttgcc ggacaaccgg aataagtcgg cgtaaagccg
    38821 atcacccatc cttctcccat ctgtgtcaca ttacgcacca tgcctaaatc ggtaatggtc
    38881 agcaccggga tctccgggtc cgggatctgg ctgagtagct cccatatctc atgaacctgt
    38941 ggcggtgcaa tggtggccag acgttgcata cccatctcct ctgttaccat tgctgaccgg
    39001 gcaagacacg ctggagatac tgcatttctg ccagcatggg tccaaggtgt tcggtatgca
    39061 gtcctttttt accgccagtg cgatacgcct gctcttgcgg tacgttcaat gtggcttcgt
    39121 tgattccggc aaaaacttcg gcttcccacg ctgcgcgtaa agtgcgtgga tcaaccgcaa
    39181 taccctcttc actcagtgca atatcaatct cgtcggcatc gaacagttcg gcagtaaaac
    39241 gccacaactt gtcgatggcc tgctgcatct tttgccctga tacgtcagta ccattgccca
    39301 gtcgctccag ccagccacga ctaaaacgca ggtgataacg cgcttcttta attgctttgg
    39361 cagaaatcgc cgccagttgc ggatcacggc tttccatcag acgggtaaag agcgccacat
    39421 gccatgcatc gatgaaatac tggcgtgcaa tggtgtcggc aaaattgccg tttggttgtt
    39481 caaccaacaa taagttgctg aactggcgct cgtctcgggt gaaggccagg gtatcttcat
    39541 caccttctcc cgctaattca gcggcatacg ataagaagtt acgtgcctga cctaataaat
    39601 cgaggccaat gtttgccagt gcgagatcga tttccagttc cggtgcgtga ccgcaccatt
    39661 cccccaaccg ctgggagagc accagacagt tatcgcccag gcgcaaggtg taagccgtta
    39721 actgattcat ttccgacctc acatgtgctc aatgccatca gggatggtgt aaaacgttgg
    39781 atggcgatag accttgcttt cagccggatc aaaaaattca ccgcgttctt ccggttgcga
    39841 ggcaacaatt tcactcgcct tcaccaccca aattgaacat ccttcgctac gacgggtgta
    39901 agcatcacgg gcattttcca gtgccatccg ctcatcggca gcatgtaaac tgccaacatg
    39961 gcggtgtgat aagccctgtt tgccacgcac gaaaacttcg tataacggcc agtaaacatt
    40021 actcattttg aatctcctta tgcgacctta cgggcaagtt gtttttgtgc atgggccagc
    40081 gctgcttccc gtacccaggt accttcttcc caggctttac gtttggcgtc gagcctctcc
    40141 tgattacaaa ttccgcgacc gttaattact tcgttaaact cctgccagtc gatctcacca
    40201 aagcggtagt gaccgctttc agtgtcaaaa tgcagatccg ggtcaggaac ggtcataccg
    40261 agcatttcaa cctgtggaat ggtgttatcc acgaaacgct ggcggagttc gtcgttggtg
    40321 aaacgtttga ttttccaggc gagacttctg gcgctgtttg gcgagttatc atcgtttggc
    40381 ccgaacatca ttaaggctgg ccaccagaaa cggttaatgg cgtcttgcaa catctgcttt
    40441 tgagcttcgc tgccctgcgc cagtgccatg caggcttcaa acccctgacg ctggtgaaaa
    40501 ctctcttctt tgcagatttt caccattgct ctggcatacg ggccataaga agtgcgacat
    40561 aacgccacct gattgacgat cgctgcgcca tccaccagcc agccgataac gccgatatca
    40621 gcccaactca atgtcggata attaaagatg gaggaatatt tcatccgtcc gtcgagcatt
    40681 ttttggtaga tgtcttctct ggcgcagccc agtgtttcgg cggcgctata gagatacaga
    40741 ccatgaccgg cttcatcctg cactttggcc aacagaatgg ctttacgccg caaggttggt
    40801 gcgcgagtga tccagttacc ttcaggcaac ataccaacaa tctcggagtg cgcatgctgc
    40861 ccaatctggc ggatcagtgt ctttcggtaa gcatcgggca tccagtcctg tggctcgata
    40921 gccgtttcct gggctatccg ttgctcaaag cgttcttctt gggtcacgtc atcaccttta
    40981 cgattccagt tttcgtgaat taattaaaaa cacgaatcac attgttttat gaaagttaca
    41041 caatggttaa ctaaaatcac aaattttgta tgcttgaact gtgacggatt tcgcacccta
    41101 tttttataaa tcattattta tcagtgcatt aatgttttcc gcagttgcta tacagatcgc
    41161 atagttaaca tttcgttaaa agatcccttg ctttttatga ttcgcgattt aactattagc
    41221 aacagaaatg tgaaacatct ggagagtagc gatgcagcag ttagccagtt tcttatccgg
    41281 tacctggcag tctggccggg gccgtagccg tttgattcat cacgctatta gcggcgaggc
    41341 gttatgggaa gtgaccagtg aaggtcttga tatggcggct gcccgccagt ttgccattga
    41401 aaaaggtgcc cccgccctcc gcgcgatgac ctttatcgaa cgtgcggcga tgcttaaagc
    41461 ggtcgctaaa catctgctga gtgaaaaaga gcgcttctat gctctttctg cgcaaacagg
    41521 cgcaacgcgg gcagacagtt gggttgatat tgaaggtggc attgggacgt tatttactta
    41581 cgccagcctc ggtagccggg agctgcctga cgatacgctg tggccggaag atgaattgat
    41641 ccccttatcg aaagaaggtg gatttgccgc gcgccatgta ctgacctcaa agtcaggcgt
    41701 ggcagtgcat attaacgcct ttaacttccc ctgctgggga atgctggaaa agctggcacc
    41761 aacgtggctg ggcggaatgc cagccatcat caaaccagct accgcgacgg cccaactgac
    41821 tcaggcgatg gtgaaatcaa ttgtcgatag tggtcttgtt cccgaaggcg caattagtct
    41881 gatctgcggt agtgctggcg acttgttgga tcatctggac agccaggatg tggtgacttt
    41941 cacggggtca gcggcgaccg gacagatgct gcgagttcag ccaaatatcg tcgccaaatc
    42001 tatccccttc actatggaag ctgattccct gaactgctgc gtactgggcg aagatgtcac
    42061 cccggatcaa ccggagtttg cgctgtttat tcgtgaagtt gtgcgtgaga tgaccacaaa
    42121 agccgggcaa aaatgtacgg caatccggcg gattattgtg ccgcaggcat tggttaatgc
    42181 tgtcagtgat gctctggttg cgcgattaca gaaagtcgtg gtcggtgatc ctgctcagga
    42241 aggcgtgaaa atgggcgcac tggtaaatgc tgagcagcgt gccgatgtgc aggaaaaagt
    42301 gaacatattg ctggctgcag gatgcgagat tcgcctcggt ggtcaggcgg atttatctgc
    42361 tgcgggtgcc ttcttcccgc caaccttatt gtactgtccg cagccggatg aaacaccggc
    42421 ggtacatgca acagaagcct ttggccctgt cgcaacgctg atgccagcac aaaaccagca
    42481 acatgctctg caactggctt gtgcaggcgg cggtagcctt gcgggaacgc tggtgacggc
    42541 tgatccgcaa attgcgcgtc agtttattgc cggcgcggca cgtacgcatg ggcgaattca
    42601 gatcctcaat gaagagtcgg caaaagaatc caccgggcat ggctccccac tgccacaact
    42661 ggtacatggt gggcctggtc gcgcaggagg cggtgaagaa ttaggtggtt tacgagcggt
    42721 gaaacattac atgcagcgaa ccgctgttca gggtagtccg acgatgcttg cagctatcag
    42781 taaacagtgg gtgcgtggtg cgaaagtcga agaagatcgt attcatccgt tccgcaaata
    42841 ttttgaggag ctacaaccag gcgacagcct gctgactccc cgccgcacaa tgacagaggc
    42901 cgatattgtt aactttgctt gcctcagcgg cgatcatttc tatgcgcata tggataagat
    42961 tgctgctgcc gaatctattt tcggtgagcg tgtggtgcat gggtattttg tgctttctgc
    43021 ggctgcgggt ctgtttgtcg atgccggtgt cggtccggtc attgctaatt atgggctgga
    43081 aaacttgcgc tttatcgaac ccgtaaagcc aggcgatacc atccaggtgc gtctcacctg
    43141 taagcgcaag acgctgaaaa aacagcgtag cgcagaagaa aaaccaacag gtgtggtgga
    43201 atgggcggta gaggtattca atcagcatca aaccccggtg gcgctgtatt caattctgac
    43261 gctggtggcc aggcagcacg gtgattttgt cgattaatcg gtgaatgaag ggcaacggcg
    43321 aatagttgcc cttttatttc actaaatttt gtgacgttgt cacattatgc atgatgtgta
    43381 catctatttt cagggcatcc actgtatgaa aagctggcac acctgccaaa caacctggca
    43441 ggtgcaggca atcccctttg catcagtact gataatgtga acctgactaa accgcccaca
    43501 gagcgcggtt gctaacaaga acacaacatc tgacgaggtt aataatggga agcccctctc
    43561 tgtattctgc ccgtaaaaca accctggcgt tggcagtcgc cttaagtttc gcctggcaag
    43621 cgccggtatt tgcacacggt ggtgaagcgc atatggtgcc aatggataaa acgcttaaag
    43681 aatttggtgc cgatgtgcag tgggacgact acgcccagct ctttaccctg attaaagatg
    43741 gcgcgtacgt gaaagtgaag cctggtgcgc aaacagcaat tgttaatggt cagcctctgg
    43801 cactgcaagt accggtagtg atgaaagaca ataaagcctg ggtttctgac acctttatta
    43861 acgatgtttt ccagtccggg ctggatcaaa cctttcaggt agaaaagcgc cttcacccac
    43921 ttaatgcgct aactgcggac gaaattaaac aggccgttga aattgttaaa gcttccgcgg
    43981 acttcaaacc caatacccgt tttactgaga tctccctgct accgccagat aaagaagctg
    44041 tctgggcgtt tgcgctggaa aacaaaccgg ttgaccagcc gcgcaaagcc gacgtcatta
    44101 tgctcgacgg caaacatatc atcgaagcgg tggtggatct gcaaaacaac aaactgctct
    44161 cctggcaacc cattaaagac gcccacggta tggtgttgct ggatgatttc gccagtgtgc
    44221 agaacattat taacaacagt gaagaatttg ccgctgccgt gaagaaacgc ggtattactg
    44281 atgcgaaaaa agtgattacc acgccgctga ccgtaggtta tttcgatggt aaagatggcc
    44341 tgaaacaaga tgcccggttg ctcaaagtca tcagctatct tgatgtcggt gatggcaact
    44401 actgggcaca tcccatcgaa aacctggtgg cggtcgttga tttagaacag aaaaaaatcg
    44461 ttaagattga agaaggtccg gtagttccgg tgccaatgac cgcacgccca tttgatggcc
    44521 gtgaccgcgt tgctccggca gttaagccta tgcaaatcat tgagcctgaa ggtaaaaatt
    44581 acaccattac tggcgatatg attcactggc ggaactggga ttttcacctc agcatgaact
    44641 ctcgcgtcgg gccgatgatc tccaccgtga cttataacga caatggcacc aaacgcaaag
    44701 tcatgtacga aggttctctc ggcggcatga ttgtgcctta cggtgatcct gatattggct
    44761 ggtactttaa agcgtatctg gactctggtg actacggtat gggcacgcta acctcaccaa
    44821 ttgctcgtgg taaagatgcc ccgtctaacg cagtgctcct taatgaaacc atcgccgact
    44881 acactggcgt gccgatggag atccctcgcg ctatcgcggt atttgaacgt tatgccgggc
    44941 ctgagtataa gcatcaggaa atgggccagc ccaacgtcag taccgaacgc cgggagttag
    45001 tggtgcgctg gatcagtaca gtgggtaact atgactacat ttttgactgg atcttccatg
    45061 aaaacggcac tattggcatt gatgccggtg ctacgggcat cgaagcggtg aaaggtgtta
    45121 aagcgaaaac catgcacgat gagacggcga aagatgacac gcgctacggc acgcttatcg
    45181 atcacaatat cgtgggtact acacaccaac atatttataa tttccgcctc gatctggatg
    45241 tagatggcga gaataacagc ctggtggcga tggacccagt ggtaaaaccg aatactgccg
    45301 gtggcccacg caccagtacc atgcaagtta atcagtacaa catcggcaat gaacaggatg
    45361 ccgcacagaa atttgatccg ggcacgattc gtctgttgag taacccgaac aaagagaacc
    45421 gcatgggcaa tccggtttcc tatcaaatta ttccttatgc aggtggtact cacccggtag
    45481 caaaaggtgc ccagttcgcg ccggacgagt ggatctatca tcgtttaagc tttatggaca
    45541 agcagctctg ggtaacgcgt tatcaccctg gcgagcgttt cccggaaggc aaatatccga
    45601 accgttccac tcatgacacc ggtcttggac aatacagtaa ggataacgag tcgctggaca
    45661 acaccgacgc tgttatctgg atgaccaccg gcaccacaca tgtggcccgt gccgaagagt
    45721 ggccgattat gccgaccgaa tgggtacata ctctgcttaa accatggaac ttctttgacg
    45781 aaacgccaac gctaggggcg ctgaagaaag ataagtgatt gtttcagaca aaaaaacggc
    45841 accaggtgcc gttttttact tatgagcgaa ccagattaat accgtacaca caccgactta
    45901 gtttcacacc aaccgtccag ccagtcgggg ccaaaatcac ggcccgttcc tgactgcttc
    45961 atcccaccaa acggtaagtt agcgtcaatt aaggtatggc tgtttaccca caccgtacct
    46021 gcctgtaagc gatcgctata ttccagagcc tgggagagat tttgcgtcca gacactggca
    46081 gttaagccat attccgtgtc gtttgccagt tgtaacgcct cttctccatc cgctactcgc
    46141 accaggttta ccaccggacc aaacacctct tcacgagtta aacgcaattt agcatcggga
    46201 tttaccacca gcgttggcgc aacataatac ccctctccgg ctggtccatt cgacccgcga
    46261 atcagctctg cttgctgtgc ctgcgcatcg tcgaggaatg aacacacttt gtcgcagtgc
    46321 gcacgagaaa ccaaagggtt aatctgtgca acaggtgaca tccccggtcc cacttgcaac
    46381 gattttaccg cctgctcaaa tccactaacc agcgtgtcaa acaacggcgc ttcaatataa
    46441 attcgcgaac tggcggcgca tacttgccct tgattcagga agcttccggt catcaagcct
    46501 tcaataaccc attgcggatc agcatctttt aatacaattg ccgggttttt accgcccagt
    46561 tccagcgtta cacgcgttaa gtgatcagca gcagttctgg caataccttt tcccgtcgcg
    46621 gttgaaccgg taaaactgat tttcgcaaca tgaggatgtg acgtcagggc cgcgccgcat
    46681 acagcacctg acccggtgac gacattaaaa acgccatcag ggataccagc ctcgctggcc
    46741 agttccgcca cgcgcaacat cgtcagtggc gtggtttccg aaggcttaat cacgattgaa
    46801 cagcctgctg ccagtgctgg catcaccttc cacataccaa tcatcaacgg aaagttccat
    46861 ggcacaattc ccgccactac gccaaccggc tctttacgcg tccaggcctg ataacgcgcc
    46921 ccctggggta agggaatcga caagtccagc gttttacccg cgattttggt cgttaacccg
    46981 gcggtataac gcatccagtt cagcgtacag cccacttcaa aagcacggga aatggcaatt
    47041 gacttgcctt gctccagggt ttccagttgc gccagctcct cactgtgctg ttccaccaga
    47101 tcggcaaaac gtagcagaat acgttcacgc tctgcgggta atcgcccggc ccagcgacgc
    47161 gagacaaagg cccgccaggc agacatgact gcgttatcta catccgcttc gttggcatca
    47221 gcagtagacg caatttcttg cccggtggcc ggatcaaaga tcgccaaccg tttttcactt
    47281 tgtgcggggc caggacgacc atcaatataa agaccgtgtt gacgatcgag aaactgttgg
    47341 acctggctta atactgctac atgcggctct gtcatcactt ttccttatta tttacccagt
    47401 gtgatttcag tgtacgcagc gacaagaaaa gtcacttttt tcgctgtgac attcgctttg
    47461 tcagctgtga caagctccgc aaatcgtgac aataaccgct tatgtctcta tgcctgacaa
    47521 gacttatttc ctatagtcag gctcattagc ttcgtttatt gcaacacaaa tgcaacaata
    47581 aaaatacatt tcacagagcg aaaacgtgcc atgaaccccg cagtggataa tgagtttcag
    47641 caatggcttt cccaaatcaa tcaggtatgc ggaaatttta ccggacgcct gctgactgag
    47701 cgttacacgg gtgtactgga cacgcatttt gccaaaggac taaagctgag taccgtgaca
    47761 accagcgggg tgaatttatc ccgcacctgg caggaggtaa aaggcagcga cgatgcctgg
    47821 ttttacaccg tttttcagct tagtggtcag gcaataatgg agcaggatga gcgtcaggtg
    47881 cagattggcg ctggcgatat tacgttactc gatgcctcac gcccctgttc gctttactgg
    47941 caggagtctt ctaaacagat ttcattactt ttgccacgca ctctgctgga acaatatttt
    48001 ccccatcaaa aacctgtctg cgcagaaaga ctggacgctg acttacccat ggtgcaactc
    48061 agtcatcgcc tgttacagga gagcatgaat aatccggcac tttctgaaac agaaagtgaa
    48121 gctgcgctac aggcgatggt gtgtctgctg cgcccggtac ttcatcagcg ggaatctgtt
    48181 caacctcgtc gtgaacgtca gtttcaaaaa gtggttacgt tgatagacga taatattcgc
    48241 gaagagatat tacgcccgga gtggatagcc ggagagacag gtatgtcagt acgtagtttg
    48301 taccgaatgt ttgccgataa aggtttggtg gtcgcgcaat atattcgtaa ccgtcgtctc
    48361 gatttttgtg cagatgcgat tcgccatgcc gcagatgatg aaaaactggc aggcatcggc
    48421 tttcattggg gattttctga ccagagtcat ttttcaacgg tatttaagca acgctttggg
    48481 atgacgccag gcgagtatcg acgtaaattc cgctaactaa ttggcgttgt gtgtactgca
    48541 acgccaatta gttaaaacag atatttgact atgcattttt cagggcgaaa ggcaatttga
    48601 caggagtttt tgaatattca ccttgtttca ggaaaataga tcaatcgctt tctcctgtag
    48661 cgttatcccg atattgcggt tttgctatta aaattttcgc acccaattac cgtttatccc
    48721 ttgtacatat tcccccggtt tggcccgagc tacgagcttt tgcccggcga gtttcgcgat
    48781 atcatctaca gacacattat tttgctttgc cagttgttgg taactcgctt tacgttcggc
    48841 attaatgtcg gttactaatt tctctgtttc agcatccgtt ttcagcgcaa ccagataacc
    48901 ataaaatgtt tcacctaccc gcccctgagt tctggcttca tccagagtca atgccattac
    48961 attgctgctt accagcccaa caagaaacgc acaaagaagt aatgttttct tcatcatcgc
    49021 ctcagaaaag atcgctacga gtttcaagca gctcttcgac atctttgtct gccttgatga
    49081 tgatctcatg ctcaatttta acgttcatat tgatagtgat cggttccttg ggcgcagcga
    49141 cttcaatacg cggcgtacag gcagccagca taaaagatga cgtcaacgca gccagtaaaa
    49201 ttttcattgt ttttcctcac actcttttcc ttgcggacag tcatttcccg gcagacgtgc
    49261 gttctgctcc aaccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa
    49321 aatattctct tcatgatggt aattaaggtt taccgtaccg cttttaccat caacgcgact
    49381 ggtacctgta atgttggcct gcatggttaa taaaccgaga ttatctaagt taatttttgt
    49441 cgatgaccgg ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cagtcatatt
    49501 gtctttcacc accgcatctg cggtgtcttt atccagccgc aaggtcattg gcccgctatt
    49561 tgccagccaa ccatctttca ctatccattt ttcgttgttt agccataacg gcaatgcacc
    49621 actaaatgcc ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga
    49681 tgagagatta ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat
    49741 cagtacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaagg gttcttcttc
    49801 agtccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat
    49861 cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc
    49921 aaaacggaaa ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact
    49981 accgcctttc aacacgccgt gtcctcccgc gcggaatcct tgttcaggcg cagcagaaaa
    50041 tgcaacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccag
    50101 cggctggaat acagtcagtg attgttttgg ccaccaggcg ttaccgcgca gacgaatacc
    50161 gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa
    50221 gaggaaatac gtcggatcgc gcccatcgac gctaaatttt aaggttgatg gcggtaacac
    50281 actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg
    50341 atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac
    50401 cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca gcgtaatcgt
    50461 gctgtcatgc cactcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact
    50521 gccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat cattcgccag
    50581 cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg
    50641 caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc
    50701 atcgatgtcc agcgaatcga tgactcttcc cttcgaacgt aataacgcgc cgggctcaaa
    50761 ggccagcgtt gggtcagtca gacttccact tagctgcgcg ggtaagcggg catataaaat
    50821 gaggtccgcc tgtttcgctt caccggtcag ttgcagtggc atctgacttt tttccatgct
    50881 taattttcct ggaccaaaat taagcaccgc gttgccttta cccgcttgcc cctgggtcag
    50941 cacactcagt cgcccgctga ccagagcgtt ctcgagccct gcctgccagt tgtcgacttt
    51001 gacacccagt cggccactca aaggaaaacc tgcatacggc cagctccagc gaccatcgct
    51061 tacggtcaat tgttgacgag taatttgcca cggcaaatcg agcaacggat cgccgttatc
    51121 ccgtgccagc acaatcaatt gcccgctatt ttcctgccag tccagctctg catccaccag
    51181 tgacggttcc tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag gaagtccatc
    51241 cggcacgagc ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc
    51301 gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac
    51361 tttttcgccc tgataacgca gttgctggat atcagaggtt aatgcgagag agagttttcc
    51421 ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag
    51481 catggactgc cactgcgcga gggtttttgg agcagctggc gattgctccg tctgcggcaa
    51541 tttcgccaga caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg
    51601 atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag
    51661 atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt
    51721 cccgagcggt agccagatgc ccgccagagt gggaacccac agcccgagcg tcatcaacag
    51781 cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag
    51841 catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc
    51901 atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt
    51961 caatatcgcc atagctttca attaaatttg aaattttgta aaatattttt agtagcttaa
    52021 atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag
    52081 tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt
    52141 gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt
    52201 ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa
    52261 tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg
    52321 gggctgaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc
    52381 ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct
    52441 aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc
    52501 ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg
    52561 ctggcgttcg atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat
    52621 ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa
    52681 aactaccatc tgttgaacga agccgccttc gaacagatga aaaatggcgt gatgatcgtc
    52741 aataccagtc gcggtgcatt gattgattct caggcagcaa ttgaagcgct gaaaaatcag
    52801 aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagac
    52861 aaatccaacg acgtgatcca ggatgacgtg ttccgtcgtc tgtctgcctg ccacaacgtg
    52921 ctgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact
    52981 acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa
    53041 tcttgccgct cccctgcaat ccaggggagc tgattcagat aatccccaat gacctttcat
    53101 tctctattct taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaac
    53161 tagccgcgtt tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg
    53221 ctgttacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc
    53281 ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg
    53341 gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca
    53401 aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca
    53461 ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc
    53521 tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc
    53581 cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca
    53641 tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac
    53701 aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag
    53761 tcgcctgctg aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc
    53821 aaaacgctgc tcgcatcttt tcttcctctg atcttcaagc caaacgacac cgccataaat
    53881 aataggcagc acagagggcg acgtcgagag ctgtcctgcg cgttgccccg catttttact
    53941 tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg
    54001 gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaggat tactactggg
    54061 cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat
    54121 tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta
    54181 ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt
    54241 aaagaacgtt tggggagaca caccacgcgt agttgaaatg cagtcggaag cgggtgctat
    54301 cgctaccgtg catggcgctt tgcagacggg tgccctttca acatcgttta cgtcatcgca
    54361 gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactga cgccatttgt
    54421 cctgcatgta gcggcacgta ccgttgccac acatgcactt tctatttttg gcgatcattc
    54481 cgacgttatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcta acgtccagga
    54541 agcgcaagat tttgctctca tttcgcacat cgcaacgctg aagagccgcg tgccatttat
    54601 tcatttcttt gatggtttcc gcacgtccca cgaaatcaat aaaattgtcc cgctggccga
    54661 tgacactatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcgct
    54721 caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc
    54781 tcgcgaagcc accaacccat ggtacgacgc ggtctatgac catgttgaac aggcgatgaa
    54841 tgatttctct gccgcgacag gtcgtcagta tcagccgttt gaatattacg ggcatccgca
    54901 agcggaacgg gtgattatcc tgatgggctc tgccattggc acctgtgaag aagtggttga
    54961 tgaattgcta acccgtggcg aaaaagttgg cgtgctgaaa gttcgcctgt accgtccctt
    55021 ctccaccaaa cacttgctgc aagctctgcc gggatccgta cgcagcgtgg cggtactgga
    55081 cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtaa tgaccgcact
    55141 ggcagaagcc tttaataatg gcgagcgcga aactctgccc cgtgtcattg gtgggcgcta
    55201 tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa
    55261 cgcggctaaa ccgaaagcgc gctttacggt tggtatttac gatgatgtga ccaatctgtc
    55321 actgccgttg ccggaaaaca ccctgccaaa ctcggcgaaa ctggaagcct tgttttatgg
    55381 ccttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc
    55441 cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac
    55501 ggtttctcac cttcgcgtga gcgaacagcc gattcgttcc gcttatctca tttcccaggc
    55561 tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt
    55621 aaaacctggc ggcattttcc tgctcaacac gccgtacagc gcagatgaag tgtggtcgcg
    55681 cttgccgcag gaagttcagg ccgtgctaaa tcagaaaaaa gcgcgcttct atgtgattaa
    55741 cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat
    55801 ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg
    55861 tgcgattgcc aaaagttaca gtagcaaagg ccaggatctg gtggaacgca actggcaggc
    55921 tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg caaccggtaa atccgcacag
    55981 cgccaatcga ccgccagtgg tttccgatgc cgcccctgat ttcgtgaaaa ccgtaaccgc
    56041 agcgatgctc gcagggcttg gtgatgccct ccccgtttcg gcgctgccgc cagacggcac
    56101 ctggccgatg ggcaccactc gctgggaaaa acgcaatatc gccgaagaga tccccatctg
    56161 gaaagaggaa ctctgtaccc aatgtaacca ctgcgttgcc gcttgcccac actcagctat
    56221 tcgcgcaaaa gtggtgccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct
    56281 ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg caccggaaga
    56341 ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga
    56401 gattaaagcc atcaatatga tgtctcgcct ggaacatgtc gaagaagaga aaatcaatta
    56461 cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg
    56521 tacatcgcag ctgattacac cgctgtttga atattcaggt gcttgctccg gttgtggcga
    56581 gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaacgc
    56641 caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata ccaccgatgc
    56701 caacggtcgt gggccggcat gggcgaactc tctatttgaa gataatgccg aatttggcct
    56761 tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt
    56821 tgccgataaa atcccggcgg aattactgac ggcgttgaaa tcagacgcca cgccagaggt
    56881 tcgtcgtgaa caggttgcag ctttacgcca gcaactcaac gatgttgccg aagcacatga
    56941 actgctacgt gatgcagatg cactggtgga aaaatcaatc tggctgattg gtggtgatgg
    57001 ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt
    57061 caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg catcgaaagc
    57121 gacaccgctg ggtgcagtaa ctaaatttgg cgagcacggc aaacgtaaag cgcgtaaaga
    57181 tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc
    57241 gcagctgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct
    57301 gatcattgct tatagcccgt gtgaagagca tggttacgat ctggcactca gccacgacca
    57361 gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgctttgatc cgcgtcgtgc
    57421 cgatgaaggc aaactgccgc tggccttgga ttcacgcccg ccgtcagaag cactggaaga
    57481 aacgttactt cacgagcaac gtttccgtcg gctgaattcg cagcagccag aagtggcaga
    57541 acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat
    57601 ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga ttatccaatt
    57661 ctaaaaaaaa agcccggacg actgttcggg cttgtctttt tatatgttgg aaaatcagtg
    57721 gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat aaaggcat
//
LOCUS       DAZFFI010000018        57069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFFI010000018 DAZFFI010000000
VERSION     DAZFFI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.018"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHJ12_001337"
     CDS             complement(<1..221)
                     /locus_tag="ACHJ12_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989157.1"
                     /translation="MFDREMINENKEAFFLYVNGRGELSAQCVIKVAEKGDYLQGYSL
                     TRRAFRTFLKDRVIQMFSSDQELNKVASET"
     gene            complement(388..729)
                     /gene="ytfP"
                     /locus_tag="ACHJ12_001338"
     CDS             complement(388..729)
                     /gene="ytfP"
                     /locus_tag="ACHJ12_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4989158.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(732..4511)
                     /gene="tamB"
                     /locus_tag="ACHJ12_001339"
     CDS             complement(732..4511)
                     /gene="tamB"
                     /locus_tag="ACHJ12_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4989159.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4508..6241)
                     /gene="tamA"
                     /locus_tag="ACHJ12_001340"
     CDS             complement(4508..6241)
                     /gene="tamA"
                     /locus_tag="ACHJ12_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4989160.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6447..7085
                     /gene="msrA"
                     /locus_tag="ACHJ12_001341"
     CDS             6447..7085
                     /gene="msrA"
                     /locus_tag="ACHJ12_001341"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4989161.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7408..8751
                     /gene="paeA"
                     /locus_tag="ACHJ12_001342"
     CDS             7408..8751
                     /gene="paeA"
                     /locus_tag="ACHJ12_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4989162.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8813..9019)
                     /gene="ytfK"
                     /locus_tag="ACHJ12_001343"
     CDS             complement(8813..9019)
                     /gene="ytfK"
                     /locus_tag="ACHJ12_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4989163.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9344..9901
                     /gene="ytfJ"
                     /locus_tag="ACHJ12_001344"
     CDS             9344..9901
                     /gene="ytfJ"
                     /locus_tag="ACHJ12_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4989164.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(9891..10631)
                     /gene="cysQ"
                     /locus_tag="ACHJ12_001345"
     CDS             complement(9891..10631)
                     /gene="cysQ"
                     /locus_tag="ACHJ12_001345"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4989165.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            10821..12764
                     /gene="cpdB"
                     /locus_tag="ACHJ12_001346"
     CDS             10821..12764
                     /gene="cpdB"
                     /locus_tag="ACHJ12_001346"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4989166.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(12893..13273)
                     /gene="ytfH"
                     /locus_tag="ACHJ12_001347"
     CDS             complement(12893..13273)
                     /gene="ytfH"
                     /locus_tag="ACHJ12_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4989167.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            13362..14222
                     /gene="qorB"
                     /locus_tag="ACHJ12_001348"
     CDS             13362..14222
                     /gene="qorB"
                     /locus_tag="ACHJ12_001348"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4989168.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            14331..15296
                     /gene="ytfF"
                     /locus_tag="ACHJ12_001349"
     CDS             14331..15296
                     /gene="ytfF"
                     /locus_tag="ACHJ12_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4989169.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            15404..16066
                     /gene="ytfE"
                     /locus_tag="ACHJ12_001350"
     CDS             15404..16066
                     /gene="ytfE"
                     /locus_tag="ACHJ12_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4989170.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(16111..17523)
                     /gene="cycA"
                     /locus_tag="ACHJ12_001351"
                     /gene_synonym="dagA"
     CDS             complement(16111..17523)
                     /gene="cycA"
                     /locus_tag="ACHJ12_001351"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4989171.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(17832..18452)
                     /gene="fklB"
                     /locus_tag="ACHJ12_001352"
     CDS             complement(17832..18452)
                     /gene="fklB"
                     /locus_tag="ACHJ12_001352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4989172.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            18671..19309
                     /gene="ytfB"
                     /locus_tag="ACHJ12_001353"
     CDS             18671..19309
                     /gene="ytfB"
                     /locus_tag="ACHJ12_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4989173.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(19293..19400)
                     /locus_tag="ACHJ12_001354"
                     /pseudo
     CDS             complement(19293..19400)
                     /locus_tag="ACHJ12_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(19444..20652)
                     /locus_tag="ACHJ12_001355"
     CDS             complement(19444..20652)
                     /locus_tag="ACHJ12_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4989174.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVAGVVKNVTVSQSCGKWY
                     ISVQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIAKIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRTILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            20660..21091
                     /gene="tnpA"
                     /locus_tag="ACHJ12_001356"
     CDS             20660..21091
                     /gene="tnpA"
                     /locus_tag="ACHJ12_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO4989175.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            complement(21149..21442)
                     /locus_tag="ACHJ12_001357"
                     /pseudo
     CDS             complement(21149..21442)
                     /locus_tag="ACHJ12_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            21714..22508
                     /gene="yjfZ"
                     /locus_tag="ACHJ12_001358"
     CDS             21714..22508
                     /gene="yjfZ"
                     /locus_tag="ACHJ12_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4989176.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(22579..23028)
                     /gene="rplI"
                     /locus_tag="ACHJ12_001359"
     CDS             complement(22579..23028)
                     /gene="rplI"
                     /locus_tag="ACHJ12_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4989177.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(23070..23297)
                     /gene="rpsR"
                     /locus_tag="ACHJ12_001360"
     CDS             complement(23070..23297)
                     /gene="rpsR"
                     /locus_tag="ACHJ12_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4989178.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(23302..23616)
                     /gene="priB"
                     /locus_tag="ACHJ12_001361"
     CDS             complement(23302..23616)
                     /gene="priB"
                     /locus_tag="ACHJ12_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4989179.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(23623..24018)
                     /gene="rpsF"
                     /locus_tag="ACHJ12_001362"
     CDS             complement(23623..24018)
                     /gene="rpsF"
                     /locus_tag="ACHJ12_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4989180.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            24345..24620
                     /gene="yjfY"
                     /locus_tag="ACHJ12_001363"
     CDS             24345..24620
                     /gene="yjfY"
                     /locus_tag="ACHJ12_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4989181.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(24695..25246)
                     /locus_tag="ACHJ12_001364"
     CDS             complement(24695..25246)
                     /locus_tag="ACHJ12_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4989182.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCVV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(25343..26029)
                     /gene="ulaF"
                     /locus_tag="ACHJ12_001365"
     CDS             complement(25343..26029)
                     /gene="ulaF"
                     /locus_tag="ACHJ12_001365"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4989183.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(26029..26883)
                     /gene="ulaE"
                     /locus_tag="ACHJ12_001366"
     CDS             complement(26029..26883)
                     /gene="ulaE"
                     /locus_tag="ACHJ12_001366"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4989184.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(26893..27543)
                     /gene="ulaD"
                     /locus_tag="ACHJ12_001367"
     CDS             complement(26893..27543)
                     /gene="ulaD"
                     /locus_tag="ACHJ12_001367"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4989185.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(27557..28021)
                     /gene="ulaC"
                     /locus_tag="ACHJ12_001368"
     CDS             complement(27557..28021)
                     /gene="ulaC"
                     /locus_tag="ACHJ12_001368"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4989186.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(28031..28336)
                     /gene="ulaB"
                     /locus_tag="ACHJ12_001369"
     CDS             complement(28031..28336)
                     /gene="ulaB"
                     /locus_tag="ACHJ12_001369"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4989187.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(28352..29749)
                     /gene="ulaA"
                     /locus_tag="ACHJ12_001370"
     CDS             complement(28352..29749)
                     /gene="ulaA"
                     /locus_tag="ACHJ12_001370"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4989188.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYRVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            30104..31168
                     /gene="ulaG"
                     /locus_tag="ACHJ12_001371"
     CDS             30104..31168
                     /gene="ulaG"
                     /locus_tag="ACHJ12_001371"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO4989189.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            31276..32031
                     /gene="ulaR"
                     /locus_tag="ACHJ12_001372"
     CDS             31276..32031
                     /gene="ulaR"
                     /locus_tag="ACHJ12_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4989190.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(32028..32777)
                     /gene="yjfP"
                     /locus_tag="ACHJ12_001373"
     CDS             complement(32028..32777)
                     /gene="yjfP"
                     /locus_tag="ACHJ12_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4989191.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            32959..33288
                     /gene="bsmA"
                     /locus_tag="ACHJ12_001374"
     CDS             32959..33288
                     /gene="bsmA"
                     /locus_tag="ACHJ12_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4989192.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            33437..33712
                     /gene="yjfN"
                     /locus_tag="ACHJ12_001375"
     CDS             33437..33712
                     /gene="yjfN"
                     /locus_tag="ACHJ12_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4989193.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(33829..35454)
                     /gene="aidB"
                     /locus_tag="ACHJ12_001376"
     CDS             complement(33829..35454)
                     /gene="aidB"
                     /locus_tag="ACHJ12_001376"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4989194.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(35538..36701)
                     /gene="yjfC"
                     /locus_tag="ACHJ12_001377"
     CDS             complement(35538..36701)
                     /gene="yjfC"
                     /locus_tag="ACHJ12_001377"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4989195.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESGAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(36704..37342)
                     /gene="yjfM"
                     /locus_tag="ACHJ12_001378"
     CDS             complement(36704..37342)
                     /gene="yjfM"
                     /locus_tag="ACHJ12_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4989196.1"
                     /translation="MARKRKSRNNSKFGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(37352..37750)
                     /gene="yjfL"
                     /locus_tag="ACHJ12_001379"
     CDS             complement(37352..37750)
                     /gene="yjfL"
                     /locus_tag="ACHJ12_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4989197.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(37768..38427)
                     /gene="yjfK"
                     /locus_tag="ACHJ12_001380"
     CDS             complement(37768..38427)
                     /gene="yjfK"
                     /locus_tag="ACHJ12_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4989198.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(38478..39176)
                     /gene="yjfJ"
                     /locus_tag="ACHJ12_001381"
     CDS             complement(38478..39176)
                     /gene="yjfJ"
                     /locus_tag="ACHJ12_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4989199.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(39195..39596)
                     /gene="yjfI"
                     /locus_tag="ACHJ12_001382"
     CDS             complement(39195..39596)
                     /gene="yjfI"
                     /locus_tag="ACHJ12_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO4989200.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(39723..40454)
                     /gene="rlmB"
                     /locus_tag="ACHJ12_001383"
     CDS             complement(39723..40454)
                     /gene="rlmB"
                     /locus_tag="ACHJ12_001383"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4989201.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(40634..43075)
                     /gene="rnr"
                     /locus_tag="ACHJ12_001384"
     CDS             complement(40634..43075)
                     /gene="rnr"
                     /locus_tag="ACHJ12_001384"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4989202.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(43114..43539)
                     /gene="nsrR"
                     /locus_tag="ACHJ12_001385"
     CDS             complement(43114..43539)
                     /gene="nsrR"
                     /locus_tag="ACHJ12_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4989203.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(43744..45042)
                     /gene="purA"
                     /locus_tag="ACHJ12_001386"
     CDS             complement(43744..45042)
                     /gene="purA"
                     /locus_tag="ACHJ12_001386"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4989204.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(45146..45343)
                     /gene="yjeT"
                     /locus_tag="ACHJ12_001387"
     CDS             complement(45146..45343)
                     /gene="yjeT"
                     /locus_tag="ACHJ12_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO4989205.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(45425..46429)
                     /gene="hflC"
                     /locus_tag="ACHJ12_001388"
     CDS             complement(45425..46429)
                     /gene="hflC"
                     /locus_tag="ACHJ12_001388"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4989206.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(46432..47691)
                     /gene="hflK"
                     /locus_tag="ACHJ12_001389"
     CDS             complement(46432..47691)
                     /gene="hflK"
                     /locus_tag="ACHJ12_001389"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4989207.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(47777..49057)
                     /gene="hflX"
                     /locus_tag="ACHJ12_001390"
     CDS             complement(47777..49057)
                     /gene="hflX"
                     /locus_tag="ACHJ12_001390"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4989208.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(49133..49441)
                     /gene="hfq"
                     /locus_tag="ACHJ12_001391"
     CDS             complement(49133..49441)
                     /gene="hfq"
                     /locus_tag="ACHJ12_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4989209.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(49527..50477)
                     /gene="miaA"
                     /locus_tag="ACHJ12_001392"
     CDS             complement(49527..50477)
                     /gene="miaA"
                     /locus_tag="ACHJ12_001392"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4989210.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(50470..52317)
                     /gene="mutL"
                     /locus_tag="ACHJ12_001393"
     CDS             complement(50470..52317)
                     /gene="mutL"
                     /locus_tag="ACHJ12_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4989211.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(52327..53664)
                     /gene="amiB"
                     /locus_tag="ACHJ12_001394"
     CDS             complement(52327..53664)
                     /gene="amiB"
                     /locus_tag="ACHJ12_001394"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4989212.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKQQNGSNYTVVFTINADAPPPPPPPPVVAKRVEKPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(53683..54144)
                     /gene="tsaE"
                     /locus_tag="ACHJ12_001395"
     CDS             complement(53683..54144)
                     /gene="tsaE"
                     /locus_tag="ACHJ12_001395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4989213.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(54116..55663)
                     /gene="nnr"
                     /locus_tag="ACHJ12_001396"
     CDS             complement(54116..55663)
                     /gene="nnr"
                     /locus_tag="ACHJ12_001396"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4989214.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            55662..56801
                     /gene="queG"
                     /locus_tag="ACHJ12_001397"
     CDS             55662..56801
                     /gene="queG"
                     /locus_tag="ACHJ12_001397"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4989215.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(56784..56837)
                     /locus_tag="ACHJ12_001398"
     CDS             complement(56784..56837)
                     /locus_tag="ACHJ12_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989216.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT        13812 a        15519 c        14001 g        13737 t
ORIGIN      
        1 gtttcagacg caaccttatt caactcctgg tcgctagaaa acatctgaat aacacggtct
       61 ttcaaaaagg tacgaaatgc gcgacgagtt aaagagtatc cttgtaaata atcccctttt
      121 tcagccacct ttatcacgca ttgagccgac aactcccctc taccgttaac atacagaaaa
      181 aacgcctctt tattttcgtt tatcatttcc ctatcaaaca taggaccccc gtatcaggct
      241 taacattttg tatatgctta gcccgcgaat cattacgggc taagatgtat cgctatggca
      301 aaatgtggac aacgagaaaa ataatccttt atttttcatt gcattacatc aattttcgcg
      361 ccacccgaag gtggcgtgct tttattatta cttatccctg tctaaccagt cgccgctttc
      421 aattagcttt aatccatcga cgggtcgttg ataaacgtac atccatgcac tcccgtacgg
      481 cgtctgaatc aactggcgcg cgtattcacc tcccctggtg cgcaaggcat caagttcggc
      541 cagcgtggcg ttgtcaatac gataaacttc accgtgtact gttccgttcc ccggaactgc
      601 gcctggatag tggcccaggc tatacaactg gtagttatcg atactgaaat cgcccagtaa
      661 ctgggcattg gtcatccagt gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa
      721 tattcgcatt gctaaaactc gaactgatag agcaaatcca gtgcctggtc tacaccagac
      781 acggcttcca gatatagctt aggcatcagg cgataacgta acgtgagtgt tgctatagag
      841 tcaaatatac ccacgccgta tttcacttgc agacctggca atacatagcc gctgaccact
      901 acctgggagg agtcgcctac tccctgggtg tcgagcgcta aattgcttac gccaaacgtc
      961 tcgccgattt tacccacaat ctggccactt tgcgcaaccc ccaggccaat cagcattgag
     1021 gtcatcgccg cactgtcgct ctgatcgctc tccagccctt gtccacgtag caaataagac
     1081 aatgcagctt gttgcgacat agccgggtca gagaagatct ccgctttcgg ttcgtccgcc
     1141 agaccagtga cgcgaactcc ggcgattacg tcgtcttctg tagcatccgg gttacgaata
     1201 gcttcaatat taagatacgg ttgatctggc ggaccagaga acagtagctc acccttacgc
     1261 acaatcagat cctgaccata ggcatggaag cgcccttcag ggatgttgat ctgcccgttc
     1321 aggcccagcc cttgtttgtc ctgaacgacg ttaagatcgc ccgtcagccg cgctttcagg
     1381 ccaaaggcgt caatgcgcac attgttgcca acgtggacaa tcaggttact gttaatcgga
     1441 atcgacgccg ttttcggctc ttccggttgc aggttatcgt taagcatcac cacatcgctg
     1501 gagacgccta ctgcgctttc cggcagatcg tgcaccacga tgcgcgccca cgggacatcc
     1561 acgcgaccat cgagggtaaa caggtttggt gtagcctcga atacaacatc tggcgataca
     1621 tccattcgta ccatcggcgg cacggtgatc cgcactttac tgcctttcgc cgttactcgt
     1681 gcccgccagt tttcaatttg gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc
     1741 tgggtccgta ctgtaccggc aagcgtcgag cgcataccgt taaagttgac cgcaagctgg
     1801 ctcggctgca tatcaaacgg cataaagttg ccgtcgatat ccacacccgt aacctgaagc
     1861 tgaccaaaca actgcgggct ttgcacatca ccacccagac gcaagttggc actcaccatc
     1921 cccgctgctt tttccccacg ggtaaagatg gggtttatca tcgcaaggtt gaagttacgg
     1981 atattgacgt tgccaccaag attacggcgg ccttgcggat cggtcacctg cacctgtcca
     2041 tcaaactggc cgttattggt caggcggatg gtccagccca attcggcacg gttgttacgc
     2101 aattccgccg tcagattcag tgtctgaaac gccaccggca gcgccgcatc gttgacggtt
     2161 tgcgttacct gcacgttacg ccccgaaagg gtgatactgc cctgcggcag cccctctttc
     2221 gtggtgtccc aggcaacatc cgctttaccc gtgaagatac cgctggcctg agtggtttct
     2281 ggcataaacg gtttcagcat ggcgaggtcg aagcggttga gattcaccac cgcacgccct
     2341 tcggcccccg catcgatagt ttgcggcacg cacagttccg cattcgggtt aagccagcag
     2401 tgtggcccga tgctgatttt ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc
     2461 agcgaccacg ggccgaccgg cgtctggaag cgggtattgc taagagttcc cttccagcgt
     2521 tcttctttgc gatcaaaact tcctgccaga ttaagctgcc cggagacagg ctcgccctga
     2581 atccgcaact gtagctcgtg ctgcttttcg ctgcctttgg cattcagggt gacgaggttg
     2641 atatttacat ccggctgcga aatttgctca acgcgtacgt cgagtttccc ggcgatctga
     2701 tcggtggatt taatgtcgcc ttccacgcga acctgcgcca cggaaagttc ctgccagcgc
     2761 aggccgcgcg cggtgatatc tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc
     2821 accagccctt tcgctgtacc gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg
     2881 atggtggcat caagattgag atcttttacc cccagctcgc ctttcacttc ggcactgttt
     2941 ggcccgagtt ccagatgaag cccagggatc atccactgca tataactgtt gcctttcagc
     3001 gtaccgtcaa cgttcacttt gttctgtttg acgttaccgg tcagcttcag ttctggcacc
     3061 tccatctgcc aggtgccgcc gtacaggcta ccgcgggttt taatcaagcc attgagtttc
     3121 gacggccact ccgggaactc tttggcggtg ttaatgccat taagcgttag ctcaccgcgc
     3181 caactaatgg cctgctgcca gtcgagcaac gccttgagtt cagttttccc ttccagcgcc
     3241 gcgacggtga gtttgtcgag attgacctgc tgttcattac ctttggcatc aagggtaatg
     3301 gtggctggcg ggatctcctg tcccttcact gccgtacgca tagagagcgt gtaatcggtc
     3361 attttgccgg taagtttcag tttcagatca tccgcctgat actgcttctc accagtgaac
     3421 ggccagtaaa gctgtttgct gttcacttcc acgttgagcg gcaatccggc ttccgccagt
     3481 cgcgtctggg cgcgtaaatc catatccacc ggaccggaaa gattaacgcc aatctccagc
     3541 tgttcgcgca gcgcgccacc cacttttagc ttcacttttt cacctttcaa cggctccacg
     3601 ttcagtgtgc tgttgagggt gatatccacc ggccagttgt ctgacagctg cgccgtgccg
     3661 ctggcgttga cgatcccctg gttggaatcg atatccaggg cgtccagttt agtattgccg
     3721 tcaatgctgc tcactttcag cagcatggtg cgcacggtga tgtccgtgtc gcccgtcacg
     3781 cgtaactgct cgcctttaaa ctcttcaatg ttcaggttaa gcggcaaatg cacgtcggtc
     3841 atttccggca ataccgggcg agaaaagaga tctttcagcg tttcgccgag cggcttttcc
     3901 tccggctgcg gattttcaat tttcggttcg accacttctt cctgcgccac ttccgccact
     3961 ttcggcagag caatcagcag gcctttcagc gacgtcggtt tcagggtcag ggttttctcc
     4021 tgccagttca ggccggaggt gaagtccatc accgacaccg tggtgtcatc aatcttgatg
     4081 ttgacgttgt ccagcgccac ccgtgtcagg gtgatgggat acggcgtgga gagatccagc
     4141 ggaccgctat cttcctcttc ttcaacctgt tcagaaggag gcattttttt actgtcgatg
     4201 ttgacctgaa tgtctttcag cgccaggtca ttaatacaaa cgctactgtt ccacaggcac
     4261 tcaagcccga ccgccagatg cagattgccc gcttttaccg ccacgcctgg ctgctcataa
     4321 cgaacgtcag acaaggtgag atcgcgccag ccgccggtga ccttgccaat atccagtcct
     4381 ggcacccagc gatccgccgc tttaaatacc agatgcaggc cgctggtggt gcccaccaga
     4441 aacgccaccg atcccagcaa cagtaagata acgataacca cgccgaggct gatttttttc
     4501 cataaactca taattctggc cccagaccga tgtaaaactg taacccgtgc tcgtctttat
     4561 ccgcgaccgg tacggcaaaa tcgagtttga ttggcccgac cggcgattcc cagcgtacgc
     4621 cgaccccggt accggtttta aagtcgctgc ggcgaatatc gcttaccgct tcgccactat
     4681 cgacaaacac cgcgccccac cattttccgg tcacgttgta ctggtattcc agcgatccgg
     4741 ttatcagctt cgaggcccct ttcaggtcac cgttggcgta tttcggggcg atagatttgt
     4801 atttgtagcc gcgaatactg cgatcgcccc cggcgaagaa acgcagatcc ggcggtactt
     4861 tgtcgaaatc acccgtttca atccagccca gcgtgccgcg tgtcacaaaa cgatggcgat
     4921 cgtacagtgt gcggatccag acgttctgcg cctggaaaac ggagaaatcg acatctgagc
     4981 cccaggccgt gttggagtag tcgatagagt agcgttgcga gtcgccccag gttggcatca
     5041 ggccaccacg agaacgcgtg cggctaatca tcaccccagg ataaaacagc atcgtggtgt
     5101 tggtaatttc accctgggta aagtggtcga ggctccagcg caggttaatg gcacgctgcc
     5161 agccgctgga gagatcccag tagcgagaag ccaccagcgt agtggagtca gattcggtat
     5221 cgttcaggtc agtgcgctta aaaccgccct gcaccaaata atattgttcc agtggattct
     5281 tcagcagcgg cattttatag ctgaagtcga gggtctgttc cggcgcggaa atactggtac
     5341 tggtggtcag actgtgacca taagagttca tccacggctt tttccacgtc gctttcacgc
     5401 gcggtcccac gtccgtagag taaccgaccc cggtttcgat ggtgttttca gttcgcggcg
     5461 aaaccacgcc cgtcaagggt aatactttcg tttcgcgcgc tttatcaaat tgtggagcca
     5521 ccaccaccga gttaaaccag ccggtagcag aaagtcggcg gttcagttcc gccagatctt
     5581 tcgattcgta ctcatcgccc tctttaaacg gcaccagatt ttgcaggtat tcatcgcgga
     5641 tttgtgatcc ttcaaaggtc acatgcccaa agcggtaacg ttcgccactg ttataatcaa
     5701 tatcccagaa ggctttatgc aggccgagcg caatgcccag ctgcgctttg gtaaattcgc
     5761 tatcgaaata acctttacgc aacgcaatgc tggttaagga ctttttgaaa ttttcataat
     5821 cgccctggtt cagcaccgtg ccaatagccg ggcgagtatc gagcaatttc aaatagtctt
     5881 tatcggtccg cgcgccgccg cgcaatacca catcggtgcc gccaattaac accggcacac
     5941 ctggcgtgac tttggcgatc aatacctgcc gccctttctt tggcggtgga cggagatcaa
     6001 attcaatggt cggctggtaa taacccagcg ctttcagacc ttcacggatg gcatcatcga
     6061 cgcgtgcgcg aaagcgacgg tctggcgtca cttcatcact ttcaatcgta gaaagctgcg
     6121 cacgaacgtt cttttccagc tgtcccgata acccctcgac ctgtagacgg acgttcgcgg
     6181 cgacggcaga tccgcttaag cagagtaagc ttacacagca taactgtcgg atatagcgca
     6241 cattttctcc tgaatatcct tttttcctgc ccctggaacg ccgttaaacg gcttaacaaa
     6301 aatccagtaa tatggattaa aaagcagact aaaccccaaa tatttcttat gttttacttt
     6361 agacctattc acggtggtta ttgtgtgcaa atacgcctct tgttacaacc ttaaccccaa
     6421 tgaccgattt tcgggagagc gacaccatga gtttatttga taaaaagcat ctggtttccc
     6481 ccgccgatgc cctgcctgga cgtaacaccc cgatgcccgt agccacgctg catgcggtca
     6541 acggtcactc aatgaccaat gtacctgacg gaatggagat tgccattttt gcgatgggtt
     6601 gtttctgggg tgtggagcgt ctgttctggc agttacccgg cgtttacagc accgccgcag
     6661 gctataccgg cggctatacg ccaaatccga cttatcggga agtgtgctcc ggtgatacgg
     6721 gtcatgccga agcggtacgc attgtttacg atccttccgt catcagctat gagcagttgc
     6781 tacaggtatt ttgggagaat cacgatcccg cccagggaat gcgtcagggc aatgaccacg
     6841 gcacgcagta tcgttcagcg atttatccgc tgaccccaga acaggatgcc gcagctcgcg
     6901 ccagtctgga acgttttcag gcggcgatgc ttgccgccga tgatgatcgt cacatcacca
     6961 cggaaatcgc taacgccaca ccgttttatt atgccgaaga tgaccaccag caatatctgc
     7021 ataaaaaccc gtatggttac tgtggaattg gcggaattgg cgtctgtctg ccaccggaag
     7081 catagcgtta cgggtacaaa tgtagattgt tgataaagtg cgctttattt atgccagatg
     7141 cggcgtaaac gccttaccag gcctacaaaa tcgtccaaat tcaatatatt gcaaggactg
     7201 cgtaggcctg attggcatag cgcatcaggc agtattgcat ttatcagcag tctgaatttt
     7261 aaccctctgg cgactttaca gtaccttacg ctatactagc cactgaaaat gccggttcac
     7321 tttcttcgaa tcggctttca atgtgtattt cacacaaatt aatcaacttc cccttccgag
     7381 gatctggcct gaaaggtcgg ataagatatg ttaaacagta ttttagtcat actctgtttg
     7441 atcgctgtaa gtgcgttctt ctcgatgtcc gagatctcgc ttgccgcctc acgcaaaatc
     7501 aaacttaaac tgctggctga tgaaggcaat ataaatgccc aacgcgttct gaatatgcag
     7561 gaaaatcccg gcatgttctt taccgtggtc caaatcggtc tgaacgcagt ggcgattctc
     7621 ggcggtatcg tcggtgatgc ggcattttct ccagcttttc acagcctgtt ctcccgctat
     7681 atgtcggcag aactctctga gcaactgagc tttattctct ctttctcgtt agtgactggc
     7741 atgtttatcc tgtttgcgga tttaaccccg aaacgcatcg gtatgattgc gccagaagcg
     7801 gtggctttgc gtatcatcaa cccgatgcgc ttctgcctgt acgtttgcac cccgctggtg
     7861 tggttcttca acggcctggc gaacatgatc ttccgtattt tcaaactgcc aatggtacgt
     7921 aaagatgaca tcacttctga tgacatctac gcggtagtgg aagccggtgc gctggcgggc
     7981 gtgttacgta aacaggaaca cgagctgatt gaaaacgtct ttgagctgga atcccgtacc
     8041 gttccgtctt caatgacacc gcgtgaaaac gtgatttggt ttgatctcca cgaagatgag
     8101 caaagcctga agaataaggt ggcggaacat ccgcactcta agttcctcgt ctgtaatgaa
     8161 gatattgacc acatcatcgg ttatgtcgat tctaaagacc tgctgaaccg cgtgctggct
     8221 aaccaaagcc tggcactgaa cagcggcgta caaattcgca acacgctgat tgtgccggat
     8281 acgttaaccc tttcagaggc gttggaaagt tttaaaaccg caggtgaaga cttcgcggtg
     8341 atcatgaacg agtacgcgct ggtggtgggg atcatcaccc tcaacgacgt gatgaccacg
     8401 ctgatgggcg atctggtcgg tcaggggctg gaagagcaga ttgtcgcccg tgatgagaac
     8461 tcatggctga ttgacggcgg caccccaatt gacgacgtca tgcgcgtgct ggatattgac
     8521 gagttcccgc agtcgggcaa ctacgaaacc atcggaggct ttatgatgtt tatgctgcgt
     8581 aagatcccga aacgcaccga ttcggtgaaa ttcgccggct acaaatttga agtggtggat
     8641 atcgataact accgcatcga ccagctgctg gtgacccgga tcgacagcaa ggccaccgcc
     8701 ctttcgccaa aactgcctga cgctaaagat aaagaagaaa gcgtcgcgta acccccagaa
     8761 acatcaacgg ctcctgaatc aggagccgtc ttattactgc atagcacttt ggttaagcca
     8821 tctctgtttg cagacgcata acctgacggt taacttcgga catcactgac aaatgcagct
     8881 tatccttcac ttttgggata agaatcttac ccttatcaaa ttcaaaagcg ccaacgtcct
     8941 tgatgtataa ccgtccacgg aacaggatct ttacgtactt cgccacctga agtgggttgt
     9001 accgttggaa aattttcatt gttatctcct gctgagtatt acgcctgtgc ggtgccacaa
     9061 tcggcccaac tattatgagg cgcaaatttt aatgcctagt gactatagac tatccgggca
     9121 atgtttccac cgcgtataac tttttttacc taaaggttac aattattcag aattatcttt
     9181 ttaccgaagc gcggtcttca gtataagcat tcatttttca tatgatttgt gcgcttgacc
     9241 gcaaactggc atcacacttg cgggaaattc gataaatagc acatatgatt aaaactcaga
     9301 cccaagtggt cggatcacct gcatatcata agaaggaaac accatgaccc tacgcaagat
     9361 tctggcactc acctgcctgc tgttgccgat gatggcttcc gcacatcagt tcgaaaccgg
     9421 tcagcgagtg ccgccgattg gcatcaccga tcgtggcgag ttggtgcttg ataaagatca
     9481 gtttagctac aaaacctgga acagcgcgca gttagtggga aaagtgcgag tactgcaaca
     9541 tattgctggt cgcacctctg caaaagagaa aaacgcgacg ctgattgaag cgattaaatc
     9601 agcgaagtta ccgcacgatc gttaccagac caccaccatt gttaacaccg acgacgcaat
     9661 tccgggttcc ggcatgtttg tgcgcagcag tctggagagt aataaaaagc tttatccctg
     9721 gtcgcagttt attgttgata gcaatggcgt cgcacgcggt gcctggcagc tggatgaaaa
     9781 aagttccgct gtagtggtgc tggataaaga cggtcgcgtg caatgggcca aagacggggc
     9841 gctcacacag gaagaggtgc agcaagtgat ggacctgctg cataaattaa ttaataaata
     9901 gacactctga accccggatt caggaacgac tcgcgcggag tgtaatccag cggtttaccc
     9961 tgccagtcgt gaacgtgcgc tccggcagcc gcagccacag catgaccagc ggcggtatcc
    10021 caaatattcg ttggcccgaa acgcgggtac agctgcgcct gtccttccgc caccaggcag
    10081 aatttcagcg aagagccgat ggacgtggtc tgatgttcgc caagctgttg cagatactct
    10141 ttcagctccg catctgcatg ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg
    10201 acctgaatct gcttgcgcac accgcactct tctttccacg ctttgccttc tgccgcgctg
    10261 tacattacgt tcattaccgg cgcatacacc acacctaaaa tcggtttgcc atggtcaatg
    10321 agcgcaatgt taacggtgaa ttcgccatta cgtttaataa actctttagt accatccagc
    10381 gggtctacca gccagtaacg ctgccagtgc tgacggactt cccaaccggg aggatcttct
    10441 tcagaaagga ccggaatatc cggtgtcagc gtacgtaaac cgtccatgat aacggtgtgt
    10501 gcggcaatat ccgctgccgt taccggagaa ttgtccgctt tgctgacgac gtccatcggt
    10561 ttcgtcccgt cgtagacctg cataatggca tcgcctgcat tccgtgcaag ctggcatact
    10621 tgatctaaca tttctccacc tcgtctctgt gagcggtgtt aacttattgt tttacttata
    10681 ccctatcgtt aatgaatgcg ccaactgtga tagtgtcatc attttcaaag cgtaaaattg
    10741 tggcattctt cactgttcta taagtaagac gtttattctc ccttttcttt cgtattcccg
    10801 atgataaaag gatgtccctg atgattaagt ttagcgcaac gctcctggcc acgctgattg
    10861 ccgccagtgt gaatgcagcg acggtcgatc tgcgtatcat ggaaaccact gatctgcata
    10921 gcaacatgat ggatttcgat tattacaaag acaccgccac ggaaaaattc ggactggtac
    10981 gtacggcaag cctgattaac gatgcccgca atgaagtgaa aaacagcgta ctggtcgata
    11041 acggcgattt gattcagggg agtccgctgg ccgattacat gtcggcgaaa ggattaaaag
    11101 caggtgatgt tcatccggtt tataaggcgc tgaatacgct ggattatacg gtcggtacac
    11161 tcggcaatca tgaatttaac tacggtctgg attacctgaa aaatgcgctg gcgggagcga
    11221 aattccctta tgtaaatgcc aacgtcattg acgccagaac caaacagcca atgtttacac
    11281 cgtatttaat taaagatacc gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga
    11341 ttggctatat tggcgtcgtg ccgccgcaaa tcatgggctg ggataaagct aatttatccg
    11401 gaaaagtgac ggtgaatgat attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg
    11461 agaaaggtgc cgatgtcgtt gtcgttctgg cgcattccgg gctgtctgcc gatccgtata
    11521 aagtaatggc ggaaaactca gtttattacc tcagtgaaat tccgggcgtt aacgccatta
    11581 tgtttggcca tgctcacgcc gttttcccgg gtaaagattt tgctgatatc gaaggggctg
    11641 atatcaccaa aggcacgctg aatggtgttc cggcggtaat gccgggcatg tggggcgatc
    11701 atcttggtgt ggtcgactta caactcagta atgacagcgg taaatggcag gtgacgcagg
    11761 cgaaagcgga agcacgaccg atttacgaca tcgctaataa aaaatccctc gcggcggaag
    11821 acagcaagct ggtagaaaca ctcaaagccg atcacgatgc cacacgccag ttcgtcagca
    11881 agccaatcgg taaatctgcc gacaatatgt atagctatct ggcactggtg caggacgatc
    11941 cgaccgtgca ggtggtgaac aacgcgcaaa aagcgtatgt cgaacattac attcagggcg
    12001 atccggatct ggcaaaactg ccggtgcttt cagctgccgc accgtttaaa gttggtggtc
    12061 gcaaaaatga cccggcaagc tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg
    12121 ccgccgatct ttatctctac cccaatacac tgattgtggt gaaagccagc ggtaaagagg
    12181 tgaaagagtg gctggagtgc tccgccggac agtttaacca gattgatcct aacagcacga
    12241 aaccacagtc actcatcaac tgggatggtt tccgcactta taactttgat gttattgatg
    12301 gtgtgaatta tcagattgat gttacccaac ccgcccgtta tgacggcgag tgccagatga
    12361 ttaatgccaa tgcagagcgg attaagaacc tgacctttaa tggcaagccg attgatccga
    12421 acgctatgtt cctggttgcc accaataact atcgcgctta cggtggcaaa tttgccggta
    12481 cgggcgacag ccatatcgct tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt
    12541 ggattgctga tgagtcgaag cgtgcggggg aaattcaccc ggcggcagat aacaactggc
    12601 gtttagcacc gattgctggc gataagaaac tggatatccg tttcgaaact tccccgtcag
    12661 ataaagccgc agcgtttatt aaagagaaag ggcaatatcc gatgaataaa gtcgcgaccg
    12721 atgatatcgg gtttgcgatt tatcaggtgg atttgagtaa gtaaaacact tctttttcgg
    12781 cctataaatc atcaaccgca tccggcattt attggcggat gcgatgctgg cgcatcttat
    12841 ccgccctaca agccatgcac cgtagaccag ataagctcag cgcatccggc agttatgccg
    12901 cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag tccgccagtg
    12961 cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc acatgcggcg
    13021 gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc tgtaacgact
    13081 gcgcaagcat cttttcgctc accccgccga ttttgcgccg caggtcgcta aagcgatgag
    13141 taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg tgtttcaaca
    13201 cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt tgcgacaggc
    13261 taacctgact catttcatac ttaccttttt gtgcgtactt actaaaagta agtttaggtg
    13321 ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct attactggtg
    13381 ccactggcca acttggtcac tatgttattg aatccttgaa gaaaacggtt cctgtcagcc
    13441 aaatagtggc tatcgttcgt aatccggcaa aagcccaggc tctggcagca caaggcatta
    13501 ccgtgcgtca ggctgactac ggcgatgaag ccgcactgac atctgcactt cagggagtgg
    13561 aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag catcgtaatg
    13621 ttattaatgc cgcaaaggcg gctggtgtga aatttatcgc ttataccagc ctgctacatg
    13681 cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa atgttggctg
    13741 attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac tacctcgcca
    13801 gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat ggcaaaatcg
    13861 cctcagcaac gcgggcagat tatgcggcag ctgcggcacg cgtgattagc gaagccggtc
    13921 acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca cagttagcgg
    13981 cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc gaagccgatt
    14041 tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg ctggcggatt
    14101 ctgacgttgg cgcatcgaaa ggcggtctgt ttgatgacag caaaacgctt agcaaattga
    14161 ttggccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat gttaataact
    14221 agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg ccgggagcaa
    14281 tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta atgattagcg
    14341 gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc gggccgttga
    14401 tcgtgccgga atacccggcg atgttgcagt cgatggggcg ttatctggcg ttagggttaa
    14461 ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg cgtcgggact
    14521 ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc tgccttgcca
    14581 gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc ctgccggtgg
    14641 tgattcccgt ttttgccaat ctgctttata gccagcgcga cggcaaactc gcatggggaa
    14701 aactcgcccc ggcactgatt tgtattggca tcggcctggc gtgtgtgaat attgctgagt
    14761 taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc gtgctggcgt
    14821 tagtttccgt ggtttgctgg gcatggtatg ccctgcgcaa cgcccgctgg ctgcgggaaa
    14881 atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcacg ctgccggttt
    14941 ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg gacttctcct
    15001 taccttttgg cccccgtccg ctggtgttta ttagtctgat gattgcgata gccgtgcttt
    15061 gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg acagtgattc
    15121 tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt ttactccgcc
    15181 agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt ggcgtggtca
    15241 tcgcggtcag agcaaaacca gaaaagcctt taactgaatc tgtctcagaa agttgacacg
    15301 ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc cttaaagatg
    15361 tatttaaaat acatcttatc tttttaagaa tgaggtatca gctatggctt atcgcgacca
    15421 acctttaggt gaactggcgc tctctattcc tcgcgcttca gctctgtttc gtaaatatga
    15481 tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac gtaaagaact
    15541 ggatgttgag gtcattgaag ctgaactggc aaagctcgct gaacaaccga ttgagaaaga
    15601 ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct accacgatcg
    15661 tcaccgcgag cagttgccgg agctgatcct gcaagcgact aaagtcgagc gcgttcacgc
    15721 cgacaaaccg agcgtgccaa aagggctgac aaaatacctg actatgctgc atgaagagct
    15781 ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac aaggcatggg
    15841 cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag cgggtgaact
    15901 gctggaagta atcaaacaca ctaccaataa cgtcacaccg ccgccggaag cctgcaccac
    15961 ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg atcacatcag
    16021 cctggaaaac aatgtactgt tcccacgcgc gctggcgggt gagtgatcta aaagctggat
    16081 ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag cccgcttctt
    16141 accaataaac agccagccca accccagcgc gataaaccac agcggggtaa ccagcagcgc
    16201 ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga acgccataca
    16261 tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttctcat gcagatgagg
    16321 acgctgtttg cggtacacca ggtacgagca aaggataatt gtccagacga acataaacag
    16381 aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat tcacatacaa
    16441 catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg cgggtactgc
    16501 gcgcttagaa agtttagcga acgctttcgg tgctacacct tcctgcgcca gaccaaacag
    16561 catacggctg gtagagaaga cgccgctgtt agcggaagac gccgcagagg tcagcaccac
    16621 aaagttgatc acgcttgcgg cagcaggcag ccctaccaac acgaacagtt caacaaacgg
    16681 gcttttctcc gggactaccg aactccacgg cgtcacggac ataattacaa tcagcgcgaa
    16741 gacgtagaac ataatgatac ggatcggaat agagttaatt gcacgcggca gtgatttctc
    16801 cggatctttg gtttccgcag cggttgtacc taccagctca atccctacga aagcgaaaac
    16861 cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc cgccatcatt
    16921 ccacaaatgc gcgaatgacg cttccacacc agtcggtgac tgaaagtgca tcgccaccat
    16981 aaccaggccg acgacaatca gcgacacaat ggcgacgatt ttgatcatcg caaaccagaa
    17041 ctccatctca ccgaacattt tcacggtggc gagattgagc gtcagcagca gcactatcac
    17101 cgccagcgag gcgacccagt cggagagatc ggggaaccag aactgagcat aagcagtgat
    17161 cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc agccggtgaa
    17221 atatcctgcc cacggcccga gtaaatcgga agcgaagtca ctaaaagatt tgtattccag
    17281 attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa aaccaatgat
    17341 catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccag accccataaa
    17401 caaccccgta ccaatggcac cgccaatggc aataagctga atatgtcggt ttgtgagatt
    17461 gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta cctgatctac
    17521 catgtttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct ttagtcagtc
    17581 tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac gcgggatgaa
    17641 tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata tcacggtgat
    17701 aacgcgaaac agctcacaaa aatacacgct tacggggtct ttgcaacaca aaatagcgcc
    17761 gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc gaggggaaca
    17821 gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt gctgaacgga
    17881 gggatggatg cgcctgcgcc gcgctcgcca tatgccagtt cctgcgggat agtcagttcc
    17941 catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat cacgccatta
    18001 accggaaatt cagcgggttc accacgagca acggagctgt caaacacggt gccgtcgatc
    18061 agtttaccgg tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc gccttcaccc
    18121 tggttgatca cgcggaattg caggccagat tcggtgctat tcacaccttc ttttttggcg
    18181 ttttcttcca ggtatttcac accttcagca gccatcgcct ggaaacgctg acgacgaacg
    18241 gcatcggcgc gctcgtggat ttcacgcagc gcgcgatgca ccacatcaac cggaacagcc
    18301 ggatgtttgc cttccagcgc atcggcaata cctgcaacca gtgcttctgg cagcagccct
    18361 tccaggccag attcactcag ttgttgccct acctgcaaac caatgccgta gcttgcttgc
    18421 gcttcgatgg tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg gtataaaagt
    18481 agcgggcagc atatcagcca cacttctttg ggtaaaactt tgtcgcagga atgatgacaa
    18541 atcccgggga aaaagaaaca atagcaatat cccgtatgaa tataataaag gcgggtttac
    18601 cacggacatc atagcgttag cttattcata ggctatgatt gaggaacaag acgcggagca
    18661 ggaggaaacc atgcccgggc gctttgaact aaaaccaacc ctggagaaag tctggcacgc
    18721 gccggacaat tttcgcttca tggacccgct gccgccgatg catcgtcggg ggatcattat
    18781 tgccgccatt gtattggtgg tcggatttct gcttccatct gatgatacgc ccaacgcgcc
    18841 ggtggtgacg cgtgaagcgc agttagatat tcagtcgcaa tcacaaccgc cgacagaaga
    18901 acagctgcgg gcacaattag tcacaccaca aaatgatcct gatcaggtgg ctccggtcgc
    18961 tccagaacct atacaggaag gccagccaga agagcagccg caaactaccc aaacgcaacc
    19021 attccagcca gatagcggaa ttgataatca atggcgctct taccgtgtcg aacccggtaa
    19081 aacgatggcg cagctgttcc gcgaccacgg actgcccgct accgatgtct atgcaatggc
    19141 gcaggtagaa ggcgcgggta agccgctgag taatttgcaa aacgggcaaa tggtgaaaat
    19201 ccgccagaac gccagcggcg tggtgacggg cttaaccatt gatacgggga ataatcaaca
    19261 ggtcctgttt acccgccagc cggatggcag ttttattcgt gcgcggtaga tgaagggtca
    19321 accaaaagcc agctaaggat tgtctctttg atgaccttac gtctggcttc cagtgcctgg
    19381 ggggaaacca tactgatatg ttgacatcct ccacgccctg aaggacgtgg atccctgctt
    19441 cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg
    19501 caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagcgccatt
    19561 tacatcagcg ttcgctgtat atccacatgc ctggcatctg aatttacttt gtgacaggcg
    19621 attttctttc gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac
    19681 agcaagcacc tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata
    19741 ccagccctga tccagtatcg tacggtttaa acctgatttt gcccggacat tgcgccccgg
    19801 ctggctgacc gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac
    19861 tatcattgcg tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt ccctgcggat
    19921 ttttgcgata caggaatgca gtcgctgtat tttgcgtttc tgcttctgcc agttgttgct
    19981 gaatttgacc ttgcggctta actgtcgctg aagtctcgcc agcttcttct ggtttttctg
    20041 aaaactgttt acaggctcaa agactgtacc atctgacagc gtggcgagtt tagccacgcc
    20101 agcatccagc ccgaccattg atgctgaagg gtgaaccgga gttgatactt cactttcagt
    20161 ctgaacactg atgtaccact taccgcagga ctggctgaca gtgacatttt tcacaacacc
    20221 cgcgacctga cggctattcc ggtagcgcat ccagccaagt ttcggcagaa aaatacggct
    20281 gttttcctga tcgagcttaa cgccctgcgg gtagcggaat gcatcattct gtccccgctt
    20341 tttgaatcgg ggaaaagcag cccgcttctg gaagaagttt ttgtaagccc gctcaaggtc
    20401 tttcagtgac tgttgcaatg gctgtgacgg ggcatcttta agccattgcg tttcagtggc
    20461 atttttccac tcaaccagcc aggaagccat tttcccgtaa gggatgtatt tattaccggc
    20521 ctcatgattc tcattctgaa gcgccagagc acgattgaaa acgaaacgac atgcgcctgc
    20581 gaagcgcctc atttcacgct cctgttgacc acctggtctt aactggaatt taaatgcctg
    20641 tagtcgtttc acaggccaaa tgatactttg gtctgtgaaa aaagaaaccg atatccggcg
    20701 tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc tttgtcacca aataccgacg
    20761 ccagattttt gaccatgacg cgacagaaaa actacgcact tacttttcaa aggtatgtgc
    20821 tgattttgaa gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc atttgttaat
    20881 taactatcct cccaaactgg cgatatccag tctggtaaac agcctcaaag gcgtatcggg
    20941 taggttactg cgacgagatc gaccagatat tgcagtcagg tattactaca aaggcgtttt
    21001 gtggagtcct ggctattttg ccagtagctg cggaggtgcg ccaatatccg ccatccgtca
    21061 atacattgaa caacagcaaa cacctggtta ggtggaaaac cgcgccgtat atccccgccc
    21121 tgaaggacgg ggttttacgg cgcaccagat aaaccaggcc aagcgtatgc tggttgatca
    21181 aataatatcc cccaagcgca gcaatattaa tattcacctg caatgggtcg atacctggct
    21241 taaaaatatt gtatttcttc ccttcatcca ataaggattc cattaactgc aagtgcgcat
    21301 ggttaatttc taagagacgc tgtgattttg cataatgcac acctttactt tgattttcac
    21361 tatgcacaat ttttaaaaac caagggttag caatataata atcccatgta aaatcaataa
    21421 ttttctcaat ggctttttgt ggctggttaa ccagatgatt aacaaacgca ccgataccaa
    21481 cagcgtagaa taattacata ccgcacaaca agaatgtatg gtggaaggcg tggaagtcat
    21541 tttctccccg ggggcatcgt tatatccata atgagcgatt tttgatatta atatctttca
    21601 ccatccgcta ttttcactac ttcagaaaac aaacttattt ttattaatat cgattctttt
    21661 tctgtgaaca tcagctgcga tatttataaa aacattaaac aggtaacatt acaatgacat
    21721 taccaacgac tatttattca tttcctgcat attcacccca tttttcgtcc accgacaaac
    21781 cagtaaaatt aaaatttcat caatatgcac gtgctacatt actgccaaat cgtggaagag
    21841 atcataattg cgatggcaga cgtactgttg agatccacaa actagatctc tctgactggc
    21901 aagcattcaa taagttagca acgaagtgta atgcttacga tggagtaaca atgaatggtg
    21961 ataattcttt tggatggaac catgaagcta cattagataa tattcacgcc cagaaataca
    22021 ataaagctta cgcgggtgcg aggttaaccg cagaactgaa atatttattg caagatgtcg
    22081 aatccttcga acccaacagt aaatacacaa tacacgaggt tgttttggga cctggttata
    22141 gtactccaga ctataccggc caaacgatag gatacgttgt aacccttcct gcccaaatgc
    22201 ctaattgctg gtcatctgaa ctaccaacaa tcgatcttta tatcgaccaa cttcgcactg
    22261 ttacaggcgt ttctaatgcc ctcggattca tcattgctgc attgctaaac gcgtattctg
    22321 atttacccca tgatttgaaa attggtctac gttccttatc aaattcagca gcaatctact
    22381 cgggtttagg tttcgagcga gtgccgcagg accgagacat aaggagtgat cgtatgcatc
    22441 taacgccagc aaaccatcct gacttatgga cacaagagaa tggtgaatgg atctacttgc
    22501 gaaactaata cctgacgaaa aatagaaagc aaaacgccga ccaatggtcg gcgtttttac
    22561 gtctcgttga ataacgaatt attcagctac tacgtttacg atcactttcg cgaatacttc
    22621 gctgtgaacc tggaagctca cttcgtgttc gccagtggta cgcagaacgc cgttcggcag
    22681 acgaacttcg ctcttagcca cttcaacgcc agctgcagtt acagcgtcag cgatgtcgcg
    22741 agtaccgatg gaaccgaaca gtttaccttc gtcgccagct ttagacgcga tggtaacagt
    22801 ttccagtgca ttgattttct cagcgcgagc attagcagct gccagaactt cagccagttt
    22861 agcttccagt tcagcgcgac gtgcttcgaa gaattcaatg tttttcttgg tagctggaac
    22921 agctttaccc tgcggtacca ggaagttacg agcatagccc gctttaacgt ttacctgatc
    22981 acccaggcta cccaggtttg ctactttatc aagcagaata acttgcatta ccttatcctc
    23041 tcaaagtcgt attaatggac cgtgaccgat tactgatggc gatcagtgta cggcagcagg
    23101 gacaggtagc gagcgcgttt gatagcgcga gccagctgac gctggtattt tgcacgggta
    23161 ccggtgatac ggcttgggac aatcttaccg ctttcggtga tgtagttttt cagcgtagcg
    23221 atatctttat agtcgatctc ttgaacgcct tccgcggtga aacggcagaa cttgcgacga
    23281 cggaaataac gtgccatatg gctagtctcc agaatctatc aattcaatct gctcggcatg
    23341 caaaaccatt ttgctcagtc cgttctttgc cttgtggcat gaaatgaacc cctgaacggt
    23401 tatgcgactg ccgaccgtta tactgtgagt aatggcctgg ttttcgtgtc cgctaacaat
    23461 aacgggcatt tggcaccacg cctgccggtg aaaaccggct tcctcctgca cagaacgatg
    23521 ctcaagcacg aactggcagt gaggaattcc tgatggactg acctttcgaa ggggagtcct
    23581 gcacacggtg ccggacaaca ccagacggtt ggtcatcaga aattactctt cagaatcccc
    23641 agcttcagca tcatcagcgg tttcgtttgc gaaatcatcg cgacgctcac ggcgctcgtc
    23701 tttcgcttta accatcggag atgcttcggt aacagcgtgc ttggtacgca taaccatgct
    23761 gcggataacg gcatcgttga agcggaaggt agtttccagc tcatcgatca cttcctgcgg
    23821 agcttcaacg ttcatcagaa cgtagtgtgc tttgtgcagt ttgttgatcg ggtaagccag
    23881 ctgacggcgg ccccagtctt ccagacggtg gatcttgcct tctgcaccag tgatggcagc
    23941 agtgtagcgc tcgatcatgc ccggaacctg ttcgctctgg tcaggatgga ccataaaaac
    24001 gatttcgtaa tgacgcatcg aattgctcct tacggattat tcagcctcct gtctgggtca
    24061 gccgaatccc ggggaggcaa ggaacgtgtt aaaggtcggc tgaaaaatga cgcgttatag
    24121 tacttgtatg ccccgctaaa ctcaagggca gagccgaaat aaatctcccc tttcacaatt
    24181 aacctctaaa ccgctgattt ggagttcatc acacttcatc atggtgtttt tttgaacaac
    24241 tgcatcagaa atggtcacca tcaacttctt tatcattgat taaaattaaa tcagaagatc
    24301 atcacagagg agagcacaca aagtcagccg tcgaggaggt atctatgttc agtcgagttt
    24361 tagcccttct ggctgtgctt ttgctaagtg caaatacatg ggcagccatt gaaattaata
    24421 accaccaggc cagaaatatg gacgacgtac agagcttagg cgtgatttat atcaatcata
    24481 atttcgccac tgaaagcgaa gcacgtcagg cattaaatga agagacagat gcgcagggcg
    24541 caacgtacta ccacgtaatt ctgatgcggg aaccggggag taacggcaat atgcacgcca
    24601 gcgcggatat ttatcgctag caccaggtat aaccaacgaa acattgccat agtttgcttt
    24661 gcccccttcg cagggggctt ttttatacgg ttattcaccg aattgcgact tccggatctg
    24721 gctcatgagt aatgcgatta cgtaagtcgc ggcgcataat ctctattatc caaaaccaga
    24781 aaatatgccc caggatttca gacgcatatt cgtcaaaagg caattgagtg agcggcggcg
    24841 ttaaattcag cgccgggcaa aatataccgt ggactgccac tgtcacaata agccctgcta
    24901 atatcccctg ccataatttc actttcggaa aaatttccgc gacaacacaa taccctatcg
    24961 caaaaaccag tgagaagatg atatgcgtca ccatcaccgg gttgataata tgttccgaga
    25021 atgtgtacac agtttgggtt gggtcgatac ctaaataatc tcgtagaaag atatacggcg
    25081 ggttaaattc atcgcgacca ccggaaaatg tgcgcggtgg caaggggact tccgctcccc
    25141 atttaacaaa tgctgaaata attcctgaaa gtacacccac aagtaacgca acgccatagc
    25201 ggcgacgttg cggatccgtt ctgacaaaca atccattgat agccataaaa acacctgttg
    25261 tttatacgat tttttaactg aagcggcccc ggaatcagac caattacatc ccgcaggagg
    25321 taataattcc ggggcgcact gcttacttct gcccgtaata ggcgttagga ccgtgcttac
    25381 gcatgaagtg tttattcatg aggaagctgt cgatgtgatt gagttgtgga ttaatactgc
    25441 gcgcaatcca cgccattttc gccacttctt ccatcactac cgcgttatgc accgcgtcgt
    25501 gggcatcttt cccccaggcg aacggtccgt gctgatacac cacaattccc ggcgtatgca
    25561 gcggctcggc gttgcccagc gtttcgataa tcactttgcc agtgttcagt tcatactcac
    25621 cctgcacctc ttcttcgctt aatccacgcg tacacgggat gtcaccaaag aagtagtcag
    25681 cgtgcgtggt gcctaacgcc gggatcgcca gccccgcctg cgcccatgcg gtagcatgag
    25741 tggagtgggt atggacaatg ccgccaagcg acggataacg acggtagagt tcaagatgcg
    25801 tcgcggtgtc ggaagatggg cgatactccc cttccaccac cttaccgctc atatcgacca
    25861 ccaccatatc gtctgctttc atggtttcgt aggcgacgcc gctgggcttg atcaccacca
    25921 gcccgcgttc gcggtcgata gcgctgacgt tgccccaggt aaaggtcacc agcccatagc
    25981 gcggcagatc catgttggct tcaaatacct gctgttttag cttttgcatt atgccgcctc
    26041 caccatgccc gctttcgcca tgcgcgcttt cacccaatca cgcgctttcg ccacttctgc
    26101 cgccgggtct tccgccgttt cgctccacat ctcaatcagg tacggcccgc aatagccact
    26161 ctgtttgagc gtttcgaaac aacgttcgaa atccactaca ccttcgccaa acggcacgtt
    26221 tttgaagacg ccaggtttgg tgtctttcac atgtaccgcg acgatatgcc cgattccggc
    26281 ctgcaactcc atctgcacat cgttgtccca cgccgacagg ttgccgatat ccgggtagag
    26341 ctggaaccac gggttgttga gatagtgcgc gtaacccagc gccttgctga tggagttcat
    26401 caacggataa tccatgatct ccatcgccag cgtcacctgc gcgcggctcg ccatctcaac
    26461 gctctctttc aggccgtcac ggaaacgacg acgcgtttcg ttattggctt cctgatagta
    26521 aacgtcatag cccgccagct ggatcacgcg aatgccgacg tcctgggcga actggatagc
    26581 tttacgcata atctccagcc cctgcgcccg caccgcgtca tcttcactgc ccagcgggaa
    26641 acgacgatga gcagaaaggc acatggacgg cacgcgcacg ccggtttcaa caatcgcatt
    26701 caccagcgcc agacgctgct cgcggctcca gtcgaggcgc gacaggcgct cgtcagtttc
    26761 atctaccgac atctcgacaa aatcgaagcc caacgttttt gccagtcgca ggcgttccag
    26821 ccagcactcc ccggcgggga gcgctttttc atagatgcca agcgggattt gtttggacaa
    26881 catatccgct ccttagcccc acagttcggc gatggaacgt ttgaactgac gcgcggcttc
    26941 aaccggagaa gcggcatcac ggatgctgcg accggcgata aacacatgaa tcggaatacc
    27001 tttgaacagc ggcagatctt ccagcgccag gccaccggtg acggtgactt tgaagcccat
    27061 atcggaaaga cgtttgatcg cggtaatgtc cgcttcgccc cacgctacgc ctgcggcctg
    27121 cgcgtcacgg ctgcggtgat aaaccacctg ctgaatgcct gcgtcacgcc actgctgcgc
    27181 ctgttcccag gtccagtagc cggtcaattc gatctgcacg tcgccgttaa actcttttgc
    27241 tacgtccagc gcgcctttgg cggtgttgat atccgcacag caaattaccg tcacccagtc
    27301 agcgttggct tcgaagcaca tacgcgaaag gattttgccc gcatcagcaa ttttagcgtc
    27361 tgccagtacg attttgtgcg ggtagagcgc tttcaggtca cgaaccgcac gcacgccttc
    27421 gcccacgcac agaatggtac ccacttcgat aatgtcgact tcttcggcaa tcaggcgagt
    27481 ggtttcgtag gcgctatcca tagtctggtt gtccagcgcg acttgcaaca tcggtaatga
    27541 catcttcaat tccttcttaa gctgccgcgt tggtgcggtc aatgagatcc agtacttcct
    27601 gctcggtacg gcaggcacgt aaacggtcga aattttcttc atcttcaaac aggttgacga
    27661 tctgcatgat gcccacttcc tggtgcgtgt tggcatcgac cgccgccatg gtgatgagga
    27721 tatccaccgg gtcgttatct tcgtggttga actccagcgg ctttttcagc gtcaccagcg
    27781 caaaaccggt tttcttaacg ccctcttccg ggcgaccgtg cggcattgcc aggcctggtg
    27841 cgagcacaaa gtacggaccg tgctgttcaa ccgcatccag aatcgcctgg tagtaacgcg
    27901 gctcgacaac atccgccgcc accagcaggt caacgccgat ttttactgcg tcctgccatg
    27961 tctcagcttc agcctgcagg cggatggatt tattctccgc cagcgaatca cgtaatttca
    28021 tggcgcgtcc ttacttcaca tcctgcggga aatgcgcttt gatcacttcc agcagtttcg
    28081 ggccaaagtc ggcaggagag agcatgttgc gcacgccaac cacgtatttg ttgccggtca
    28141 cggtgatttc gcccgcaatg tgcgtagaag cgatgatgat atccgcgccg ctcaactcgc
    28201 ttttgtattc gccaaccgcg cagctattta ccgtgtggtc aatgtttgat tgggttaaaa
    28261 actggtccac tttcatcttc atgatcatgg aactgccttg tccgttgcca cacacagcca
    28321 gaatacgtac ggtcataatc aaaactcctt attaagcaga ctgttctgcc agttgttttt
    28381 ctgcatcttc ttctgcgcgc agtgcgcggc cagcgaagaa catataagcc agtgcaatta
    28441 caatgatgac ggccataaag gcgataccga tggagaagaa gccctgcatc atcggcggtg
    28501 ccagaatcga ccagtccgcc atgcccatcc aggcgctcat accggtgagt ttcaccgccc
    28561 agacgcagcc aaagatttca atcatcccca tcaccagaca tatcttcagc gccgcacgcc
    28621 agccgccgaa gtggttagcg aacacgccga tggtggcgtt ggagaagaac atcgggataa
    28681 agccgggaat aatcaggatc gaggaaccgc aggcgaccag gatgccaacc gcaatcagct
    28741 gaccgatggt gccccacata aagccccaaa ccacggcgtt cggcgcgaag ctatagatag
    28801 ccgcacagtc aatcgccaga accgcaccag ggatcaggcg ctgggagata ccgttaaatg
    28861 cttcagagag ttccgccaca aacatgcgca cgccctgagt gatgatgaag atcgccaccg
    28921 cgaaggagaa accagtttgc aggatgtaca ccgtccagtt cactttgcct gccatcgcct
    28981 gcacggtgtc gataccgaag gagagcagaa tggcaccaaa gaagatggtc atcacaatcg
    29041 ccgtggagac gatgttgtcg tggaaaatat tcagccagcc tggcagtttg aggtcttcaa
    29101 cgctttcctc ttttttgccg aggaacggcg caactctata ggcaatccat gatgcaaact
    29161 gttgctggtg accgatggag aaaccgcagc cgtccgtcac ttcctgagtc ggcttgtaca
    29221 tcatgttgga ggtaatgccc cagtagagcg aaaccagaat cgccgtgcag ataatggttg
    29281 tccacatgga gtagccgaaa ataaacaacg taacggcaat cagccccgcc tgctggaaca
    29341 tgatatgacc agtcagcatg atggtacgaa taccggtaat gcgacgcagc agcacgtaac
    29401 agatgttcag cgccagcgct aacaacaccg cgtaacctac ccagctatag gcatcgccca
    29461 tgcggtcgat ggttgccatc atcgacgcgt aagtatcgga aattgcgccg ttaatgccgt
    29521 agacttcgga cattttcgcc accaccggtt tgaaggtgct ggtgaggatg ccggaccctg
    29581 cctgcaacaa catgaaacca attatggttt taatcgtgcc tttaataata acgctgacac
    29641 ttttgcgcag taggatgtag cccagacagg tcacaatacc cagcaacaac ggggcattgg
    29701 tcatgacctg gttaaaaaac acggtaaaga tgttgtagag gatctccata acgatctcct
    29761 gaagaagagg taaccgggta ttccacacac ccggaatgtt gtgcactcac tctaattttc
    29821 aaaagtaatc acaacaagat tatttatgat taaatgtgac gcgacccgca aataattcca
    29881 catggataga atgggattca cacaacgaaa caactattca tttaaattca tttagttaat
    29941 tgaattacac attccattaa atctttccac actcaaaatc aacaaccact tccctggcaa
    30001 aaagtcaaaa aatggtcatt gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa
    30061 tcacacattg attcgaattg aacaatcatg aaggtaaatg gcgatgagta aagtgaaaag
    30121 tatcacccgt gaatcctgga tcctgagcac tttcccggag tggggtagct ggttgaatga
    30181 agaaattgaa caagaacagg tcgctccagg cacatttgcg atgtggtggc ttggctgcac
    30241 cgggatctgg ttgaaatcgg aaggtggcac caacgtttgc gttgatttct ggtgcggcac
    30301 tggcaaacaa agtcacggta acccgttaat gaaacagggt caccagatgc agcgcatggc
    30361 tggcgtgaaa aaactgcagc caaacctgcg caccaccccg tttgttcttg atccgtttgc
    30421 gattcgccag atcgacgcgg tactggcgac tcacgatcac aacgatcata tcgacgttaa
    30481 cgttgctgct gccgtgatgc agaactgtgc tgatgacgta ccgttcattg gaccgaaaac
    30541 ctgtgtggat ttgtggattg gctggggcgt accgaaagag cgttgcatcg tggtcaaacc
    30601 gggcgatgta gtaaaagtga aagacattga aattcatgcg cttgacgctt tcgaccgtac
    30661 tgcactgatc accctgcctg ccgatcaaaa agcggctggc gtactgccag atggcatgga
    30721 cgatcgcgca gtaaactacc tgttcaaaac ccctggcggt tccctatatc acagtggcga
    30781 ttcccactac tccaactatt atgcgaagca cggtaacgaa catcagatcg atgtggcgtt
    30841 aggatcgtat ggtgagaacc cgcgcggtat taccgacaaa atgaccagcg ccgatatgct
    30901 gcgtatgggt gaagccctga atgcgaaagt ggttatcccg ttccaccacg acatctggtc
    30961 aaacttccag gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa aagatcgtct
    31021 gaagtatggc ttcaagccgt ttatctggca ggttggcggc aaatttacct ggccgctgga
    31081 taaagacaac ttcgagtacc actatccgcg cggtttcgat gattgcttca ctattgaacc
    31141 ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat accggatagc gtggagaagt
    31201 tcgcgctatc cggcgtagta tttcctggct atttcaaata tcatctaaaa aaatcaaatt
    31261 ttatcggaat agctcatgac tgaagcacaa agacatcaaa tcctcctgga aatgctcgca
    31321 caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc gcctgccact
    31381 gcgcgacgcg atatcaataa acttgacgaa agcggcaaac tgaaaaaagt gcgcaatggc
    31441 gcagaagcta ttacccaaca gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag
    31501 aatcacgatg aaaaagtacg tatcgctaaa gcggcctcgc agctggttaa tccgggcgaa
    31561 agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag
    31621 ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt acctgatcga tcaagaacat
    31681 gacagcgtga tcattatggg cggacagtac aacaaaagtc agtccatcac tttaagcccg
    31741 cagggcagcg aaaacagtct ctatgccggg cactggatgt ttaccagcgg caaaggactg
    31801 acagcagaag ggttgtataa aaccgatatg ctgacggcaa tggcagagca gaagatgctg
    31861 agcgtggtag ggaaactggt ggtactggtt gatagcagta agattggcga acgcgcggga
    31921 atgcttttta gccgtgccga tcaaattgat atgctgatca ccggcaaaaa tgctaacccg
    31981 gaaatcctgc aacaactgga agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg
    32041 cggaaaaatg tcactgcagc atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc
    32101 tgccatgaac aggttagcag tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt
    32161 agtgattcgt cggcaggcac aacgtcatcg tcgaggccat gccacagcag tagaggtctg
    32221 tcactaagtt gttccaggtg gtttgtcgct tcccactctg ccagcggcgc gacaatgtta
    32281 ttgaattcat tctgctgtgc tgtcgtttca ggtatcagcg gtggaaacag tgaacgggcg
    32341 agtgatgtaa aatagcccga tcccatcatg ctggcggtac atctcaccgt ggggtggcga
    32401 gcggtaatcc ccagtgccgt catcgcgccc atcgacgcac caccgactgc cagacggtca
    32461 tcaagcagcc agttttcttc ggctattgcc gcacgtaaag tagtgaattc ctgcatactt
    32521 tgtagcaaga tttgccagaa ttgatttaac cgccgcgctg cgtcaccact aaaacggcta
    32581 ccgtgatcgg gcgcatccgg catgatcact cgcaaaccag cctgcgccag cgcaacggca
    32641 aaatagctat acaccagact ggatgaagta aagccgtgat aaaaaattac gcacggtaac
    32701 ggggtatctt tttgcccgac aggataagca tgaagaacgg gaatatctgc cagctcgcgt
    32761 gattctattt caatcatctg tctctccttt ttgtctggta tgacatgaag aaatccttag
    32821 cacaagtttc tgctggcggt attagttcat aatgttgaga tgtgggttac gctttcgtca
    32881 cgttttcatt cgaaaatcgc gtaccaggta acaattcggg aacattcccc caaagttaaa
    32941 ttaataaggc actacactat ggttagcagg aaacgtaata gcgttattta ccggtttgcc
    33001 agtttattat tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac gccacagcca
    33061 gcaccgccag taacggacca tccgcaagag attcgccgcg accagacgca agggttacag
    33121 cgaataggca gcgtaagcac gatggttcgg ggttctccgg atgacgcatt agcagaaatc
    33181 agagcgaaag ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt ggacgaaacc
    33241 atcgtgacag gacagtggta ttcacaagcc attttgtacc gtaaataaat cagacaactt
    33301 acatagtcag ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac aataaattac
    33361 cggctggaag cctgggcgtt tattaccgcg atggaatgcc ctgcaacatg tggggaaacg
    33421 aaaaatggag ctgacgatga aacaattact tgcctcaccc tcgctgcaat tagtgactta
    33481 tcctgcgagc gccacggcac agtctgcaga attcgccagt gctgactgcg tgacgggcct
    33541 gaatgaaata ggtcagatct cagttagcaa tatttcaggg gatccgcagg acgtggaacg
    33601 tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta ttaccatgta
    33661 cgaagaccag cagcctgata actggcgcgt acaggcgata ctgtatgcat gagtgtcatc
    33721 tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt gtccggccta caattttcac
    33781 atatttcatt tagttaatcg aaaccagcgt cgcatcagtc gtatacgctt acacacacac
    33841 tccccccgtc gcccgcagca ataaatcatt ctggatctgc tctgacaacc gtacgccgcc
    33901 gcgcgtatct aacatcacct gacaccacgc ctgcgccatt ggcggagaag catatttcaa
    33961 catttgcgca ccgcagccca gcaggaatag ctgatgagta atctctcgcc tcagttcttc
    34021 agctggctta cgcagctgct gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg
    34081 ccctttcact tccacaaacg cttccgacaa taagtcgtat acgcccgctt gcttattgag
    34141 aacgcgcagc acatccaggc acataatatt gccggaacct tcccaaatac tgtttaccgg
    34201 catctcccgg taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac
    34261 ctccatcgct tcggccacaa acggcatacc tcttttgcag atcacaaatt ttgccgcagg
    34321 cgtaaataaa cgcgcccaca gggcttcttt ggcatcggca cgccggtccc acgctcgcgc
    34381 aagacgaaac agcaacgccg tttgcccttc aagctgaagt gccatgcgac ttaagacatg
    34441 acgcataagg ggctgttgga tcaatggatt accaaaaaca tggcgttgat gtgcatgata
    34501 aatcgccagc gaaaatgcac ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg
    34561 cgtcatccca cccattttca ggatcagacg aattccttcc ccttcctgcc ccaacaacca
    34621 gccaatggca tcctgaaact ccacttcgca gctggcgtta gagcgattac ccagcttatc
    34681 tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa
    34741 aaagcaggac aaaccgcccg tagtctgcgc cagcaccaga tgcgcatcgc tttgcggcac
    34801 cgagaaaaac catttatgcc ccaccagccg ataagagcca tcttccaggc gctctgcgcg
    34861 ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa
    34921 caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc tcagcagcgg
    34981 cgtggtccag tcctgaaacg gcgcgggtaa catctgtaac agcaatggcg tggcggcaaa
    35041 ggtcatggtt atcggacata acgaccctgc ctcaacctgt gcgtgcaaca taaaacgcgc
    35101 cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc caggcaagat tgtgcacccg
    35161 attggtacat agcgcctgca tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag
    35221 acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga ttcacattcg ccagccgccc
    35281 cagttcaagg gattcagccg ttcctaactg ctgaccaata ctggctagaa aatcgctatc
    35341 ccagccggca ccttcacgcg ttaccgcttc gcagagcgcg ccatcagaca ggtataagtt
    35401 gctgttattt aatggtatag gttgattaaa aacggtgtga gtttgccagt gcactgtgtc
    35461 tccctccatc aatggcagtc accattagta tggtcactgc catggattcc tgacagaaag
    35521 agattgctaa aacattctta gccagcaatg taatgcggaa cgaaacgaga ggtgtctttg
    35581 gtgatcagcg tgttatcttc acggatcccc attccgcacg cttcatcatc gacaatccag
    35641 ctaccgatga gtgtgtagct atcgccaaat cgcggcagag gttgaaacgc ctggtagatc
    35701 atcggttcat cggcgtaatc accatcagcg tggtcaacga cgttattctg accgtcgaaa
    35761 atggtgacgt ttccgccttc gcgcgagtag atcggtttac gcacatagct ttcgccagcg
    35821 gcaatctgcg atttttcacc ctcaaaccag gatgcaagaa ggttaggatg accagggaag
    35881 aagcgccaaa gcagcggcat tagcccttta ttactcaaga tacttttcca taacggctcc
    35941 acccattgct cgcgacgctt gcacagcagc ggaccgttat cgtcacgcat catccactcc
    36001 agcggataca gcttaaatgc acgctggatg acattatcat caagatcggt cagtacaccg
    36061 ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg actcctgccc tgcctgctga
    36121 gcgcagtcct gcaagtacag cacggtagtc ctgtcttcat cggtgtcctg acagcagcag
    36181 aaataaaacg gctcccggct ataaagctcg ctaaagcgtg aaatcaggcg ttcctgaatg
    36241 gcattgtact gatcggcatc acgcggaata atgccgctgc gccgggcatc ctccagccac
    36301 aaccactgga aataagccga ctcatacaac gaggtcggcg tatcggcgtt gtactccagc
    36361 agcttcaccg gcgcattgcc acaccaggca aaatccatcc ggccatacag cgaaggatcg
    36421 cgtgcgcgcc agctttcggc gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc
    36481 aggatctctt cgtctttaac cgcgcgatcc accacctcaa ggcacatctg atgcagttcc
    36541 gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag taaaacggta agccccactc
    36601 tcgtcccaat agatttcatt gtcgatgata tgaaagtcga aaccgttgtt agcggcgatc
    36661 tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct ccccaatgcc
    36721 cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac ggttgacgct tttttggtgg
    36781 taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag gagtaatcac
    36841 cggaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg ttatagacga
    36901 acggctcatc gcgatcttta cgaataactc ggcttaacaa aaatccagaa acgaccggga
    36961 tccaactctg ttcaacattg tcgtagtagc aattttcgta cttagactga caattctgct
    37021 gagtcatatt cttcggaaca tcggcataaa atgccgcttt ggcgttgttc cagccacgcg
    37081 cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc
    37141 cgtcgccgtc gttatcgaca tcgccacttt cactccatcc tttgactatt aaaaaaacgg
    37201 caccgcccat tagcaccaac gttaagtatt tttgtgcgta gcgattgcgg cgcggctcaa
    37261 aaggattatg cggtcttcca atgcgactga tcgctccgtg accaaattta ctgttatttc
    37321 ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct gcgttaaaaa
    37381 taccaccagc cagtgcggcg gttcccataa acattcctgc tgcggtattg tgattaataa
    37441 ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt aactgaatca
    37501 ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg attgccgcac
    37561 tggataaggg gatcacgtaa cccaacaatg taccgctgaa tgccagtgac gccgcggtat
    37621 tattgttttt gattaattgc cattcgttgt gcggtgtaat tttggagtag ataaacagaa
    37681 aaataatcac catagccacg ccaataaaaa aataggcact aaaggcaaga agtgaatcca
    37741 gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg tcagcgggat
    37801 atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt taaaagattc
    37861 ctcaccattt aacagcaaat attcgtaagt atcttcggtt acttggcgtt ggtagcccat
    37921 ggtaaaatta tggacctccc atttggcatg gttttgattt tctacttttt ccagcatgta
    37981 gacgggttcg atatttcccg gttcttcgct gttaaaaaat cgctgccagc gtttttcctg
    38041 ccagtttaag atcattgccc ccatcacttt ggcgttgatc gcctcgcgcc agtgactctc
    38101 tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa tgtcatcaat
    38161 atcttcgccg ccggtggtat tgatttgcag aaattcatcg cctgaagtgt agtagcggaa
    38221 aatctgacta ccgccgccca gatcgatgtg gctgacagcg gctaccgtaa attcctcacc
    38281 cggcagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt cgagcgtaaa
    38341 gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat tatccttgcc
    38401 aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc agccttttag
    38461 cgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg
    38521 gcgctacttt tattgctacc gccaattccg gcttccgcca gcttttcatc aagatcgcga
    38581 ccgtctgcga ctttttccaa ctgtgcagca gcatccaggc gagcctgacg ttcggcctga
    38641 cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg
    38701 gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc
    38761 atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt ttccacgcca
    38821 tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag attctcaagg
    38881 cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt gcttaacgct
    38941 tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag cttatcgtga
    39001 cttaatttca cccgcgccag cagatcaacg cgagattttc cggctttatc gagttcagct
    39061 ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc aatggattct
    39121 tccgcctggg agataaacga tttccccagc gtaaataaac tttttaaaat tcccataaat
    39181 acccgtcctt gtaattagtg tgaatattct tctgtaattt cagccagatc caatgcgtta
    39241 tctaccagcg aggttatctc aagcaggata tcttcaagag aagatttaag cgataacgcc
    39301 ccgaaaacaa tgtaatactc ttcctgttgc acgctggaga ttcctaccga tgacaacggc
    39361 atcatcttct gatttcttaa taagaaggta ttaaattcat ctggattgct gatactactt
    39421 accggacaaa taaaggtttc gataatcatt tggcgggaag taaaaagaat attgatttgc
    39481 aaatcgccat aatcattcat tttaataatt aatgcgctct cgctatttgt cacatcaata
    39541 ttttgttcag gtacagtttg cagcgccgtc gccagcgcca acggattcca tgtcatatat
    39601 tattccatat agatttagtt taaatattaa taaaatgaat atttgcagta cgtaattatc
    39661 ttaccagcta tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagacg
    39721 aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc
    39781 agggaagaaa cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc
    39841 aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg
    39901 ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc
    39961 tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc
    40021 gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca
    40081 atcaccgcgt gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga
    40141 tcggttacac cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
    40201 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
    40261 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
    40321 gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag
    40381 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
    40441 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
    40501 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
    40561 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
    40621 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
    40681 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg
    40741 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
    40801 taccgacctg acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
    40861 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
    40921 tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag
    40981 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
    41041 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
    41101 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
    41161 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
    41221 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
    41281 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt
    41341 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
    41401 ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca
    41461 gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt
    41521 agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg
    41581 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
    41641 ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
    41701 ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt
    41761 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt
    41821 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact
    41881 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
    41941 cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa
    42001 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt
    42061 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac
    42121 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
    42181 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa
    42241 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaga tcaacacggc
    42301 ccgcttttgc ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct
    42361 gcggccagat gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
    42421 ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
    42481 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg
    42541 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
    42601 cagtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
    42661 cttcacgacg acctttacgg tcagcgccca gcggctgcgc cagcacctga tcgccatgaa
    42721 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
    42781 gaaagccgta gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg
    42841 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
    42901 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
    42961 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
    43021 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
    43081 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
    43141 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
    43201 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
    43261 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
    43321 cgcactcgcc gatttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
    43381 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
    43441 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
    43501 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
    43561 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
    43621 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
    43681 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
    43741 gaattacgcg tcgaacggat cgcgcagaat catggtttca gtacgatccg gaccggtaga
    43801 gatgatatcg atcggcacac cagtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
    43861 ctgcggcaga ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt
    43921 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc
    43981 gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac
    44041 gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc
    44101 aacggtgtcc agccagccgg tacgacgacg acgccccgta gttgcgccga attcgttacc
    44161 ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcacc
    44221 tacacgagtg gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag
    44281 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
    44341 accgtggtcg atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg
    44401 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
    44461 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
    44521 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
    44581 gtcgaaaagg tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc
    44641 gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc
    44701 cagcgcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
    44761 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
    44821 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
    44881 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
    44941 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
    45001 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
    45061 gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
    45121 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
    45181 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
    45241 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
    45301 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
    45361 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
    45421 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
    45481 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
    45541 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
    45601 catcgccttc accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca
    45661 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
    45721 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
    45781 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
    45841 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
    45901 cggcaatggc gttatccgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg
    45961 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt
    46021 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
    46081 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
    46141 tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt
    46201 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
    46261 gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta
    46321 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
    46381 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
    46441 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
    46501 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg
    46561 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
    46621 ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt
    46681 gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
    46741 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
    46801 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
    46861 cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
    46921 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
    46981 gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg
    47041 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
    47101 accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt
    47161 cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg
    47221 cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc
    47281 cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
    47341 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
    47401 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
    47461 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
    47521 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
    47581 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
    47641 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
    47701 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
    47761 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
    47821 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
    47881 actctttttc tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggcg
    47941 gcaaacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa
    48001 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
    48061 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
    48121 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
    48181 cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
    48241 cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta
    48301 cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt
    48361 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
    48421 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
    48481 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgt gactgtatct
    48541 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
    48601 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
    48661 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
    48721 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
    48781 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg
    48841 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
    48901 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
    48961 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
    49021 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
    49081 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg
    49141 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
    49201 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag
    49261 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
    49321 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
    49381 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
    49441 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
    49501 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    49561 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
    49621 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    49681 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
    49741 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    49801 aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg
    49861 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    49921 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    49981 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga
    50041 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg
    50101 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    50161 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    50221 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    50281 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    50341 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    50401 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    50461 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    50521 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgctccacgt ccgccagcag
    50581 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    50641 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    50701 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    50761 ctgtgcatct gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa
    50821 tgccgatttt tcttcggcag aaacttttag ccgcaacgga atcagcagcg gctgggcgca
    50881 aacgggcgct tcacccggcg tcaattgtgc ctgacgtagc caacgttctg ccactggcaa
    50941 ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat ggacgatagt
    51001 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    51061 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    51121 tttctggtag cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg
    51181 cgcaggagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg
    51241 ccccgccgcc acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt catcgtccag
    51301 cggtagcggc gtttccagtt gctgctgtag cacgctcagc acgccctgat agataaagtc
    51361 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc
    51421 cacctgatgc gggtcgatct ccagatacaa cacaaacgcc ggttgttgat cggcccccag
    51481 tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc
    51541 attcacgtag caatactgaa tttccgccag tgcgggcgta gtgtgatttg gatcggccac
    51601 ccagccgcgc agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    51661 agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    51721 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    51781 cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg tgcgcaggaa
    51841 tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg ttcccaccgg
    51901 atgcgccgcc ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc
    51961 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    52021 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    52081 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    52141 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    52201 cgcacctgca tcgaggctgt tttccactag ttctttgact accgacgcag gtcgctcgac
    52261 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    52321 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt
    52381 tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaag taattgcgca ggcctttgta
    52441 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    52501 gttgctgata aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc
    52561 gagactcgcg tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat
    52621 catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac
    52681 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag
    52741 cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg
    52801 gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    52861 aatcgacacg aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga
    52921 aataaagtaa tccccgtcac gggttaaaac gcctttaaac attggatcgt cattgagcaa
    52981 agtacgcaat ttacgtgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    53041 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    53101 agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    53161 agtgcggtta ctttcggttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    53221 aaccgcaggt ttttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcgc
    53281 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgctgct tcaccgcttc
    53341 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    53401 tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat
    53461 cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    53521 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg
    53581 aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa
    53641 ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc
    53701 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    53761 ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca
    53821 ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc
    53881 ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata
    53941 gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa
    54001 aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    54061 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg
    54121 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    54181 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    54241 cgtcagctgc agcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt
    54301 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca
    54361 tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    54421 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta
    54481 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat
    54541 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat
    54601 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc
    54661 actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg
    54721 taagagagtc catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg
    54781 caatgttttc actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt
    54841 cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc
    54901 cgtgatcgcc tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg
    54961 ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt
    55021 caaaatgcag ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg
    55081 caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag
    55141 ccagcaggcc ggaagggata tcaaccgccg caatcggcgc aggatgggaa ttagcgtggt
    55201 cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat
    55261 caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc tcgcctcccg
    55321 cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct
    55381 gggccagcag cgtgacctca atgccgaccg ctgtggccag ccgcgcgacc acgtagccat
    55441 cgccgccgtt attaccatga ccgcacagca ccagccaatg gcgggcgtca ggatacgccg
    55501 aacgacacac ctggaatgcg gcttcgccag cgcgaagcat cagctcatag agtgtaagcc
    55561 ccagcgcatc tgccgcctca cgttctccgc ggcggatatc gtcggcgtgc cagacggtgt
    55621 gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc
    55681 tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag
    55741 gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac
    55801 aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg
    55861 aattattgcc cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg
    55921 ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg
    55981 gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc
    56041 aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc
    56101 cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg
    56161 aggccggttc tttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc
    56221 aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg
    56281 ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac
    56341 ttgaaggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg
    56401 atgactgcca gcttatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt
    56461 tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag
    56521 agaagttttt aaaagtcacg gaaggatcgg cgattcgtcg tattggtcac ctgcgttggc
    56581 tgcgtaatat cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc
    56641 tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc
    56701 agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc
    56761 tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa
    56821 taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca
    56881 aaacggaatc ttaattttta acttatctat caataggtta aaaaatacta ttcaccgtgc
    56941 gaagtttctt tgaaattaag attacagcct gaggctgtgg ataactctgt ttacaagagt
    57001 ttgtcagaga caacgaaaac cagcaataac gcatcgcatc gctgtggata ttttattgag
    57061 agaagaatt
//
LOCUS       DAZFFI010000019        56957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFFI010000019 DAZFFI010000000
VERSION     DAZFFI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.019"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..836
                     /gene="fnr"
                     /locus_tag="ACHJ12_001399"
     CDS             84..836
                     /gene="fnr"
                     /locus_tag="ACHJ12_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4989217.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            988..1938
                     /gene="uspE"
                     /locus_tag="ACHJ12_001400"
     CDS             988..1938
                     /gene="uspE"
                     /locus_tag="ACHJ12_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4989218.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKEMIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1988..2245)
                     /gene="ynaJ"
                     /locus_tag="ACHJ12_001401"
     CDS             complement(1988..2245)
                     /gene="ynaJ"
                     /locus_tag="ACHJ12_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4989219.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            2489..3520
                     /gene="ynaI"
                     /locus_tag="ACHJ12_001402"
     CDS             2489..3520
                     /gene="ynaI"
                     /locus_tag="ACHJ12_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4989220.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(3571..5184)
                     /gene="mppA"
                     /locus_tag="ACHJ12_001403"
     CDS             complement(3571..5184)
                     /gene="mppA"
                     /locus_tag="ACHJ12_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4989221.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(5521..6420)
                     /gene="pgrR"
                     /locus_tag="ACHJ12_001404"
     CDS             complement(5521..6420)
                     /gene="pgrR"
                     /locus_tag="ACHJ12_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4989222.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            6558..7478
                     /locus_tag="ACHJ12_001405"
     CDS             6558..7478
                     /locus_tag="ACHJ12_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4989223.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            7478..8410
                     /locus_tag="ACHJ12_001406"
     CDS             7478..8410
                     /locus_tag="ACHJ12_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4989224.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            8428..9066
                     /locus_tag="ACHJ12_001407"
     CDS             8428..9066
                     /locus_tag="ACHJ12_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4989225.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            9376..10104
                     /gene="mpaA"
                     /locus_tag="ACHJ12_001408"
     CDS             9376..10104
                     /gene="mpaA"
                     /locus_tag="ACHJ12_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4989226.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(10079..11044)
                     /gene="ycjG"
                     /locus_tag="ACHJ12_001409"
     CDS             complement(10079..11044)
                     /gene="ycjG"
                     /locus_tag="ACHJ12_001409"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4989227.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            11163..11669
                     /gene="tpx"
                     /locus_tag="ACHJ12_001410"
     CDS             11163..11669
                     /gene="tpx"
                     /locus_tag="ACHJ12_001410"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4989228.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(11713..13254)
                     /gene="tyrR"
                     /locus_tag="ACHJ12_001411"
     CDS             complement(11713..13254)
                     /gene="tyrR"
                     /locus_tag="ACHJ12_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4989229.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(13402..14463)
                     /gene="ycjF"
                     /locus_tag="ACHJ12_001412"
     CDS             complement(13402..14463)
                     /gene="ycjF"
                     /locus_tag="ACHJ12_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4989230.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(14460..15857)
                     /gene="ycjX"
                     /locus_tag="ACHJ12_001413"
     CDS             complement(14460..15857)
                     /gene="ycjX"
                     /locus_tag="ACHJ12_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4989231.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            16012..17010
                     /gene="ycjW"
                     /locus_tag="ACHJ12_001414"
     CDS             16012..17010
                     /gene="ycjW"
                     /locus_tag="ACHJ12_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4989232.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(17121..18026)
                     /gene="ompG"
                     /locus_tag="ACHJ12_001415"
     CDS             complement(17121..18026)
                     /gene="ompG"
                     /locus_tag="ACHJ12_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4989233.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(18071..19153)
                     /locus_tag="ACHJ12_001416"
     CDS             complement(18071..19153)
                     /locus_tag="ACHJ12_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4989234.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(19167..19826)
                     /gene="pgmB"
                     /locus_tag="ACHJ12_001417"
     CDS             complement(19167..19826)
                     /gene="pgmB"
                     /locus_tag="ACHJ12_001417"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4989235.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(19823..22090)
                     /gene="ycjT"
                     /locus_tag="ACHJ12_001418"
     CDS             complement(19823..22090)
                     /gene="ycjT"
                     /locus_tag="ACHJ12_001418"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4989236.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLAFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(22087..23142)
                     /gene="ycjS"
                     /locus_tag="ACHJ12_001419"
     CDS             complement(22087..23142)
                     /gene="ycjS"
                     /locus_tag="ACHJ12_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4989237.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(23152..23940)
                     /gene="ycjR"
                     /locus_tag="ACHJ12_001420"
     CDS             complement(23152..23940)
                     /gene="ycjR"
                     /locus_tag="ACHJ12_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4989238.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(23958..25010)
                     /gene="ycjQ"
                     /locus_tag="ACHJ12_001421"
     CDS             complement(23958..25010)
                     /gene="ycjQ"
                     /locus_tag="ACHJ12_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4989239.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(25041..25883)
                     /gene="ycjP"
                     /locus_tag="ACHJ12_001422"
     CDS             complement(25041..25883)
                     /gene="ycjP"
                     /locus_tag="ACHJ12_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4989240.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(25870..26751)
                     /gene="ycjO"
                     /locus_tag="ACHJ12_001423"
     CDS             complement(25870..26751)
                     /gene="ycjO"
                     /locus_tag="ACHJ12_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4989241.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(26772..28064)
                     /gene="ycjN"
                     /locus_tag="ACHJ12_001424"
     CDS             complement(26772..28064)
                     /gene="ycjN"
                     /locus_tag="ACHJ12_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4989242.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(28078..29757)
                     /gene="ycjM"
                     /locus_tag="ACHJ12_001425"
     CDS             complement(28078..29757)
                     /gene="ycjM"
                     /locus_tag="ACHJ12_001425"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO4989243.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(29970..30284)
                     /gene="pspE"
                     /locus_tag="ACHJ12_001426"
     CDS             complement(29970..30284)
                     /gene="pspE"
                     /locus_tag="ACHJ12_001426"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4989244.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(30359..30580)
                     /gene="pspD"
                     /locus_tag="ACHJ12_001427"
     CDS             complement(30359..30580)
                     /gene="pspD"
                     /locus_tag="ACHJ12_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4989245.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(30589..30948)
                     /gene="pspC"
                     /locus_tag="ACHJ12_001428"
     CDS             complement(30589..30948)
                     /gene="pspC"
                     /locus_tag="ACHJ12_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4989246.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(30948..31172)
                     /gene="pspB"
                     /locus_tag="ACHJ12_001429"
     CDS             complement(30948..31172)
                     /gene="pspB"
                     /locus_tag="ACHJ12_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4989247.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(31226..31894)
                     /gene="pspA"
                     /locus_tag="ACHJ12_001430"
     CDS             complement(31226..31894)
                     /gene="pspA"
                     /locus_tag="ACHJ12_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4989248.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            32061..33038
                     /gene="pspF"
                     /locus_tag="ACHJ12_001431"
     CDS             32061..33038
                     /gene="pspF"
                     /locus_tag="ACHJ12_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4989249.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(33158..34423)
                     /gene="puuE"
                     /locus_tag="ACHJ12_001432"
     CDS             complement(33158..34423)
                     /gene="puuE"
                     /locus_tag="ACHJ12_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4989250.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(34461..35741)
                     /gene="puuB"
                     /locus_tag="ACHJ12_001433"
     CDS             complement(34461..35741)
                     /gene="puuB"
                     /locus_tag="ACHJ12_001433"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4989251.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(35743..37230)
                     /gene="puuC"
                     /locus_tag="ACHJ12_001434"
     CDS             complement(35743..37230)
                     /gene="puuC"
                     /locus_tag="ACHJ12_001434"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4989252.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(37505..38062)
                     /gene="puuR"
                     /locus_tag="ACHJ12_001435"
     CDS             complement(37505..38062)
                     /gene="puuR"
                     /locus_tag="ACHJ12_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4989253.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(38089..38853)
                     /gene="puuD"
                     /locus_tag="ACHJ12_001436"
     CDS             complement(38089..38853)
                     /gene="puuD"
                     /locus_tag="ACHJ12_001436"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO4989254.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            39065..40483
                     /gene="puuA"
                     /locus_tag="ACHJ12_001437"
     CDS             39065..40483
                     /gene="puuA"
                     /locus_tag="ACHJ12_001437"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4989255.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            40632..40796
                     /locus_tag="ACHJ12_001438"
     CDS             40632..40796
                     /locus_tag="ACHJ12_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989256.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            40786..42171
                     /gene="puuP"
                     /locus_tag="ACHJ12_001439"
     CDS             40786..42171
                     /gene="puuP"
                     /locus_tag="ACHJ12_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4989257.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            42305..42550
                     /gene="ymjA"
                     /locus_tag="ACHJ12_001440"
     CDS             42305..42550
                     /gene="ymjA"
                     /locus_tag="ACHJ12_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO4989258.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            42863..44506
                     /gene="sapA"
                     /locus_tag="ACHJ12_001441"
     CDS             42863..44506
                     /gene="sapA"
                     /locus_tag="ACHJ12_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4989259.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            44503..45468
                     /gene="sapB"
                     /locus_tag="ACHJ12_001442"
     CDS             44503..45468
                     /gene="sapB"
                     /locus_tag="ACHJ12_001442"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4989260.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            45455..46345
                     /gene="sapC"
                     /locus_tag="ACHJ12_001443"
     CDS             45455..46345
                     /gene="sapC"
                     /locus_tag="ACHJ12_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4989261.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            46345..47337
                     /gene="sapD"
                     /locus_tag="ACHJ12_001444"
     CDS             46345..47337
                     /gene="sapD"
                     /locus_tag="ACHJ12_001444"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4989262.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            47339..48145
                     /gene="sapF"
                     /locus_tag="ACHJ12_001445"
     CDS             47339..48145
                     /gene="sapF"
                     /locus_tag="ACHJ12_001445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4989263.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            48213..48566
                     /gene="ycjD"
                     /locus_tag="ACHJ12_001446"
     CDS             48213..48566
                     /gene="ycjD"
                     /locus_tag="ACHJ12_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO4989264.1"
                     /translation="MMQKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            48936..49724
                     /gene="fabI"
                     /locus_tag="ACHJ12_001447"
     CDS             48936..49724
                     /gene="fabI"
                     /locus_tag="ACHJ12_001447"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4989265.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            49868..50995
                     /gene="yciW"
                     /locus_tag="ACHJ12_001448"
     CDS             49868..50995
                     /gene="yciW"
                     /locus_tag="ACHJ12_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO4989266.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            51063..52997
                     /gene="rnb"
                     /locus_tag="ACHJ12_001449"
     CDS             51063..52997
                     /gene="rnb"
                     /locus_tag="ACHJ12_001449"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4989267.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(53075..53179)
                     /locus_tag="ACHJ12_001450"
     CDS             complement(53075..53179)
                     /locus_tag="ACHJ12_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989268.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            53233..55218
                     /gene="pdeR"
                     /locus_tag="ACHJ12_001451"
     CDS             53233..55218
                     /gene="pdeR"
                     /locus_tag="ACHJ12_001451"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4989269.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(55200..55328)
                     /gene="ymiD"
                     /locus_tag="ACHJ12_001452"
     CDS             complement(55200..55328)
                     /gene="ymiD"
                     /locus_tag="ACHJ12_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO4989270.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            55365..55538
                     /gene="yciZ"
                     /locus_tag="ACHJ12_001453"
     CDS             55365..55538
                     /gene="yciZ"
                     /locus_tag="ACHJ12_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4989271.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            55628..56377
                     /gene="yciT"
                     /locus_tag="ACHJ12_001454"
     CDS             55628..56377
                     /gene="yciT"
                     /locus_tag="ACHJ12_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4989272.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVYF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            56646..56864
                     /gene="osmB"
                     /locus_tag="ACHJ12_001455"
     CDS             56646..56864
                     /gene="osmB"
                     /locus_tag="ACHJ12_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4989273.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT        13754 a        14833 c        14132 g        14238 t
ORIGIN      
        1 tgctgttaag gtttgcttag acttacttgc tccctaaaaa gatgttaaaa ttgacaaata
       61 tcaattacgg cttgagcaga cctatgatcc cggaaaagcg aattatacgg cgcattcagt
      121 ctggcggttg tgctatccat tgccaggatt gcagcatcag ccagctttgc atcccgttca
      181 cactcaacga acatgagctt gatcagcttg ataatatcat tgagcggaag aagcctattc
      241 agaaaggcca gacgctgttt aaggctggtg atgaacttaa atcgctttat gccatccgct
      301 ccggtacgat taaaagttat accatcactg agcaaggcga cgagcaaatc actggtttcc
      361 atttagcagg cgacctggtg ggatttgacg ccatcggcag cggccatcac ccgagcttcg
      421 cgcaggcgct ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt
      481 ccggtaaaat gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag
      541 gcgatcagga catgatcttg ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat
      601 tcatctacaa cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt gaattccgcc
      661 tgacgatgac tcgtggcgat atcggtaact atctgggcct gacggtagaa accatcagcc
      721 gtctgctggg tcgcttccag aaaagcggta tgctggcagt caaaggtaaa tacatcacca
      781 tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt gcctgatttt
      841 tccgcataac tcactatcct tctgtcatat cattaaattt ttctgattta ttgatctggc
      901 agaaggttca tcactgtttc attcaccaga tatgggttaa tcttttaatt acaaactgcg
      961 ttgacagttg ttgtaaggag accctgtatg gctatgtatc agaacatgct cgttgttatc
     1021 gatcctaacc aggacgacca accagcattg cggcgagctg tttatttaca tcaacggatt
     1081 ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct catacgaaat gaccaccctg
     1141 ctctccccgg acgaacgtac cgctatgcgt cagggcgtca tcagccagcg tacagcctgg
     1201 atccacgagc aggcaaaata ttatctcaat gctggcgttc ccattgaaat taaagtggtc
     1261 tggcataacc gtcccttcga agccatcatt caggaagtga tcagcggcgg gcacgatttg
     1321 gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg tgatttttac gccaacggac
     1381 tggcatctgt tacgcaaatg cccaagcccg gtgtggatgg tgaaagacca gccgtggccg
     1441 gaaggaggca aggcgctggt ggcggtgaat ctcgccagtg aagagccgta tcataatgcg
     1501 ctcaatgaaa aactggtcaa agagatgatc gaactggcag aacaagtcaa ccataccgaa
     1561 gttcatctgg ttggcgctta tccggtaacg ccaattaata tcgcgattga actgccggaa
     1621 tttgatccga gcgtttataa cgatgccatt cgtgggcaac atttgctggc aatgaaagct
     1681 ctgcggcaga aattcggcat taatgaaaac atgacgcacg tagaaaaagg tctgccagaa
     1741 gaggtgattc ctgatttggc ggagcactta caggcgggga ttgtggttct gggcacggta
     1801 ggacgcaccg gtatttcagc agcattcctc ggcaacacgg cggaacaggt gattgatcat
     1861 cttcgctgcg atctgttggt cattaaacct gaccagtatc agacacccgt tgaactggat
     1921 gacgaagaag acgattaacg actcttcaat aactgacaag gcccgagtta tcgggcctat
     1981 tttttactta ctgctcttcg ccgccaagga aataaattcc caacgggatt gccagcaaca
     2041 cagtaaagac caggctataa acaaaaatca tcgatgactg aatcacatac attgatgtcg
     2101 tccactcaga cagcggaata ttgtactgtt ctataacgcc gccgatagtc gcgcgagtca
     2161 caaccgacgc cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc ttccctttcg
     2221 ctgacttcag tttcgccata atcatgatgt aatccttgtg cagacaaatt tcttgttacc
     2281 taaacgtaac aataccatga catttcaccg ttgtctgctc tgaaaacctc acctcttagc
     2341 tggatttaca atcaacgcat tgattagtta gctaatattg ctacttcctt ccctgactat
     2401 tgatgatctg aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc ggcatgacga
     2461 caattatctg gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa ataatgcgct
     2521 taatctggtc attattttcg gtagctgcgc agcattgatt ctgatgagct tttggtttcg
     2581 ccgcggaaat cgtaaaagaa aaggtttttt attccatgcg gtgcaatttt taatctacac
     2641 cataattatc agtgctgttg gtagcattat taattatgtt atagaaaact ataaactcaa
     2701 atttatcacc ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg ttattctgac
     2761 gattaagcta tttttgctga ttaatcagtt tgaaaaacag cagattaaaa aaggtcgcga
     2821 tatcaccagt gcgcggatta tgtcgcgcat tatcaaaatc accattattg tggtgcttgt
     2881 tctgctttat ggcgaacatt tcggcatgag cctttctggc ttgttgacct ttggtggtat
     2941 tggtggtctg gctgtcggta tggccggtaa agatattctg agtaatttct tttccgggat
     3001 catgctctat ttcgaccgtc ctttcagtat tggcgactgg atccgttcac cggacagaaa
     3061 tatcgaaggt acagtagcgg aaattggctg gcgaattacc aaaattacga cctttgataa
     3121 tcgtccattg tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa acccaggacg
     3181 aatgaccaac cgccgcatta ccacgaccat tggtttacgt tatgaggatg cggcaaaagt
     3241 gggcgttatt gtcgaagccg tacgtgagat gctgaaaaat cacccggcca tcgaccagcg
     3301 acaaacctta ctggtttatt tcaaccagtt tgccgactcg tcattgaata ttatggttta
     3361 ttgctttacc aaaaccacgg tatgggctga atggcttgct gcacagcaag acgtttattt
     3421 gaagattatc gatattgtac agtcacacgg cgcggacttt gccttcccca gccagacgct
     3481 gtatatggat aacattacac cgccggaaca gggtcgctaa atcagacatc actggcgtta
     3541 tccacaaggg taacgccagt tcccatcaca tcaatgcttc acaatataca tagtccgact
     3601 gtacgccaca tcttcagggt tattaatggg ataacctttc agccacggtt tgattaatcg
     3661 tccattggta tattgataaa ttggtgcaat cggtgcttgc tccatgagga ttttttctgc
     3721 cgcgttgtaa tcggcattac gcgctttaac ggtattttcc gtcgatgcct gggccagaac
     3781 tttgtcatat gccggattgt taaagcgtga aatatttcct gaatgcgttg acgttaataa
     3841 tgtcaggaaa gtggagggtt cattataatc ccccacccat gaggcgcgga taacatcaaa
     3901 attgccggtg ttacggctat cgatataggt tttccattcc tgattttgca atttaacatc
     3961 tacgccaagg ttctttttcc acatcgatgc tacagcaatc gcaatttttt gatggttttc
     4021 tgaagtgtta tacaaaagcg tcagcttcag cggtttttgc ggaccataac cagctgcgct
     4081 caacaaagtt tttgcctgcg cattcagttc ttcctgactc atttgttcaa acggcgaagg
     4141 ttccggcgta aatccggcgg taacatctgg tgtaaaatgc cacgctggct tttcgcccgt
     4201 ccctaatact ttttcggtca tcaggcggcg atctatcgtc atacttaatg ccagacgaac
     4261 gcgctgatct gccgtcggcc ctttttgcgt gttaaacgca taataatagg tcccgagctg
     4321 tggcggcgta taaacctgcc ccggaatatc cttcaacagc ttctgataca tatttttcgg
     4381 gaaggattcg gtgatatcaa tatcccccgc gaggtaacgc ttagtggctg cggattcctg
     4441 attaattggc aggaaggtca ctttttgcag taccgttttg gcgttatccc aataatgggt
     4501 attcggtacc acgaccagtt tttcattgac tacgcgatct ttaagaacat aagcgccatt
     4561 gccgatcaga tttccgggtt tcgtccactc tttaccgctt tctacgttgg ctttttgcac
     4621 cgggaagaag gcaaagttag cggttaaatt cacaaaccac ggcaacggtt tatcaagctg
     4681 aattttcaag gtatgggcat caactgcggt gacgccaagc tggtcaggcg tagctttacc
     4741 atcaataatc gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg caaatggcga
     4801 tagcgttttc gggtccacca gacgctgcca gctgtagaca aaatcttgcg ccgttaccgg
     4861 tgtgccatcc gcccattttg cgttatcgcg cagggtaaaa gtccagatac ggttgtcatt
     4921 acttttccac tgagtcgcaa cgccggggac aatctcccct ttctcgttct gattcaccag
     4981 tccttcaaac agatcgcgaa tgacctgaat ctctggcagg cccacggctt tagcgggatc
     5041 cagcgacgca ggctcatctt taatgtggcg caccagctcc tgcttctctg ccagtactgt
     5101 gccgctcgga acttctgcag catacgaaag agaaatgctg ctgaccaaca gcgcacaaca
     5161 cgtgactgaa acagagtgct tcataagttt ccctctaacc gacattaaaa ttgatgcgta
     5221 attatttgtt tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg acacaggaag
     5281 cataaaatgt tttatgtatc aatatattaa atcagattta gtgtaaataa tcgacgccaa
     5341 aatgcggtaa agcacataga acaccgaaca ctacgttcag gcttcgcacg ttcaaaccag
     5401 ttttcggtat cataatcagc atctgtcgcc attgtacgaa gaataaaaaa taaccaaaac
     5461 gttgaaagga actgagaata cgttaagaga gaaattaaat taatgtatca gcaagtagtg
     5521 ctaaacagca cctttatact tcagcctatc tataaataat gagaatgccg agccagcatg
     5581 tcgacggtgt ggatagtaca ggtgataacc gggtaaatct ggtgtgaatt tatccaaaac
     5641 acgtatcagt tttttttctt taatagcccg ctcaacctga tcataaggta gatacgccaa
     5701 tccatgccca tcaattgcag catcaatgat caggtctatc gtattcagta aaagctgacc
     5761 ttccatgcga acacgaactt cacgtccacc gcgtattaat ctccagcgat ttgctgtacc
     5821 cgatgtggga agatacaaat taattgcctg atgatctatt aattgtgaca ctgacgttgg
     5881 aacacttcgg cgagaaaaat aatccggtga gccaacaata gccattggta tatctggccc
     5941 gattcgaatg gcgatcatat ctttatccat ctccccaccc agacggacgc ctgcatcaaa
     6001 acgttcagaa acgacatcgg tcaaaccata atcaatggtg agctgaatat caatttcagg
     6061 atgcgatttc aggaatgtgc gcattgctgg taacaatatc gtttttgctg catgttctac
     6121 agtagtaata cgtattgtcc cggatgggcg gttctgcagc tcgctcaggg atgccagggc
     6181 tgaatctatg tcatgcaaca tagggccaag aacagacaaa agatgctcgc ccgcttcagt
     6241 tggaacaacg ctgcgcgtgg ttcgcgtcag aagccgcaat cccaatcgtt cttctatacg
     6301 acgcactatc tggcttaaag ctgactgcgc catgctcagg cgggctgctg cacgagtgaa
     6361 gctacgctcc tctgcaacga cgacaaacgc catcagatca gcgatttctt cacgcttcat
     6421 tcagtatctc catgcaatct attaatcatt taaatgatag gtctaatcat gattttgggt
     6481 ctaatcaact taatgatcaa tatctatagt gatatcacca accgttaaaa cgaaccgaca
     6541 actccatgag gaacggaatg atgaataata aagtcagctt cactaacagc aataatccaa
     6601 ctatctcttt gtctgcagtg atctatttcc ctccaaaatt tgatgaaacc cgtcagtatc
     6661 aggcaattgt ggtatctcac ccaggtgggg gcgttaaaga acaaacggcc ggaacctatg
     6721 ccaaaaaact ggcggaaaag ggatttgtca ctattgctta tgacgcatct tatcaaggtg
     6781 aaagtggcgg cgagccgcgt cagttagaaa acccatacat tcgtactgaa gacatcagtg
     6841 ccgtgattga ttacctgaca acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg
     6901 gaatttgtgc cggtgcagga tatacagcca atgctgcaat tcaggatcgc cgcatcaagg
     6961 ccatcggtac cgttagtgcg gtcaatattg gttcgatgtt ccgtaatggt tgggaaaaca
     7021 atgtgaaatc tattgatgcc ctgccatacg ttgaagcggg ttcaaacgcc agaaccagcg
     7081 atattagcag cggagaatac gccattatgc cactggcacc aatgaaggag tccgatgcgc
     7141 cgaacgaaga actgcgtcag gcctgggagt attaccacac ccctcgcgcg cagtatccaa
     7201 cagcaccggg ttacgctact ttgcgcagcc ttaaccagat tattacctat gatgcttacc
     7261 atatggcgga agtgtacctg actcagccga tgcagattgt ggcgggcagc caggcaggga
     7321 gcaaatggat gagtgacgac ctgtacgatc gggcatcaag ccaggataaa cgctatcaca
     7381 ttgttgaagg cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa
     7441 tttcagtatt agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa
     7501 tagtgatatg gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc
     7561 aaaacagtat ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtc
     7621 tggcaatatc tatggcaaag cattggctga aaaaggctat ctcgttctcg cttatgacgc
     7681 cagtttccag ggagaatctg gtggggagcc tcgctggata gaagatccga cacagcgcgt
     7741 agaggatatt agccgtgtta tcgattatgc agtaactctg ccatatgtta atgctgaacg
     7801 tattggcgtg ctgggagtgt gtgggggtgg tggctatgcc attaatgccg cgctgacaga
     7861 aaaacgaatt aaagcagtag tgagtattac gggtgttaat atcgggcgtc tttttcggga
     7921 aggtttcagc aattacgatc cgattggcgc attgaatgca atggcgtcgc aacgaaccaa
     7981 agaggctcgt ggtggagaat tacagatcaa cgaattactt cccgccagcc tggatgcagc
     8041 aaaagcccat ggactgaccg aacgcgatgt ttatgaagca actgactact ataaaacacc
     8101 acgcgggcaa caaccgggag gtgcgacaaa aatgttgttc tcacatgcac agaaaacgct
     8161 ggcctgggat gccttcgcat ttactgaagt gttactgact cagccggtga tggtagtagt
     8221 tggtgaaaaa gtcggtgcct tcggtgctta tcgtgatgga ctggaagtct atggccgggc
     8281 gatggtttcg caggatcgtc agttagtttc attacccgat ttctcacatt atgaactgta
     8341 tgacaaaccg gaagcagttc aggaggcgct ggaaaaagtt attccttttt tcaacaccca
     8401 tctgggataa gtttcatgag aaatattatg aaaaatgtac tgattcttgg tgctggcggt
     8461 cagatagccc gccatgtgat taatcaactg gcagataaac aaaccattaa acaaacttta
     8521 tttgcccgtc agccagcaaa aatccacaaa ccctatccaa ctaatagcaa gattattatg
     8581 ggagatgtac tgaatcatgc agcactgaag caggccatgc agggacagga cgttgtgtat
     8641 gccaacctta cgggggaaga tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa
     8701 gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg gtatttatga tgaagttcca
     8761 ggaaaatttg gcgaatggaa taatgcagtt attggtgagc ccctaaaacc attccgcagg
     8821 gctgcggatg caattgaggc ttctggactg gaatatacaa ttcttcgccc tgcctggctg
     8881 actgatgaag atattattga ttatgagctt acctcacgca atgaaccatt caaaggtacg
     8941 atcgtatcac gtaagagtgt ctcagcatta atcacagata tcatcgataa acctgagaaa
     9001 cacattggtg agaatatagg tatcaatcaa cccgtgactg atggtgacaa gccattattc
     9061 atgtaaatca attacatagt taccatcgta atgtactatg tgcagtacga tttattaaac
     9121 ttattaaggt gaactgatca atccgatgcg attaacatgt gtagaaccag taagaataaa
     9181 atacgccatg tatataacat tacgacttac atctaagtca gcataattct aatatctgca
     9241 catcaataaa aatgcaaaca acttccggca aaaaaagtga tacaagatcg atgacatagc
     9301 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     9361 gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc atttccgccc
     9421 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
     9481 gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     9541 gtcgtcacgc tctcctgtgc tctgcggacg ttgacacctt ctttgcgccg ccatcatgtg
     9601 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     9661 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
     9721 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctcagaa
     9781 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
     9841 ttccacgatc cactggcctg tattgaagat cccagacaca gcgaattagg cgaatggctg
     9901 gcccaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
     9961 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
    10021 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
    10081 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
    10141 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
    10201 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
    10261 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
    10321 catagcgccc tttcagcgcc ttcagattac tacgtgtatg acaactctca tcagcacaaa
    10381 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga aggggttgtt
    10441 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
    10501 gccaggattc atttgcatca acgatcagcg tcgcatcggg cacagctgtg cgaattgcca
    10561 ccatccgctc actaataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
    10621 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgact accgtctgtg
    10681 cagtgataac cgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
    10741 ttcgcgcggc cagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
    10801 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcgacacg acgctcataa
    10861 tttgcgccat taccgaggca tcactttccc cataacgcgg atacggcgtg cattcgccgg
    10921 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
    10981 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcgaatacc ttaacggttc
    11041 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
    11101 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
    11161 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
    11221 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
    11281 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggag
    11341 tttgcgccgc atcagtacgt aagtttaacc aactggcaac cgagatcgac aacaccgttg
    11401 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
    11461 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gccttcggtg
    11521 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
    11581 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
    11641 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
    11701 gcatattcgc gcttactctt cgttcttctt ctggcttaag ccatattccc gcaacttatt
    11761 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
    11821 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
    11881 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
    11941 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
    12001 ctgccgcaca tttcccggcc acgcatatcg cgtaagtaca gtattcaggt cagcggccag
    12061 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
    12121 catgatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
    12181 ataatagaga tcttcacgga acatgccttt ttgcaccagt tcgaccagat tcttctgcgt
    12241 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
    12301 gccatcatta aggaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
    12361 atccaacagc accgaaccac cgttcgcctg ctcaaagaat cctttcttcc cttccggagc
    12421 atgaccaaac agttcactct cgaccgcatc ttccggtata gacgcacagt tcagcgccag
    12481 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaga gatctttacc
    12541 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
    12601 ttcgacaaca tgcttcattt tcgggctgac ggcgacaatt tgactgaagg cgctgacgtc
    12661 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
    12721 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
    12781 caggaaattc tgcccattaa taacgacatg ctcgttatgg gaatcttgcg gttcgctttc
    12841 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
    12901 atccaatttt tgcgcaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
    12961 catatcgaca gagagcacag gttcgggcaa cgcctccagt aacgcgctca acgccagatg
    13021 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
    13081 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
    13141 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
    13201 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
    13261 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
    13321 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
    13381 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagcgtttc
    13441 tttcacctga ccgataagct gacgacggaa atccccgagg cgaggtttgt catcgtcaat
    13501 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg tcagaagtcc
    13561 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
    13621 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
    13681 cagcttaaac agacgcaaac ggctgtaata ccctagttca atgccataca gcgtggcgat
    13741 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
    13801 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
    13861 ggcatctaaa actggctgga ccaaatgcgc atacaaactg accacttcac ggtcgttttg
    13921 cgtttcatgg attgaggcat accagcgttg cagcgctgga tgagactgat caatacccgc
    13981 ctgctgcgcc agtttttcgc aaaatgcgcg gcctttgccc gtgccgtggc tgtgcaacaa
    14041 atcgcgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
    14101 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
    14161 cgccacccag tcctgagttt gccaggcatt cattgtccac tgtacaccct gcccgacaac
    14221 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
    14281 acgtaacgct gcatccatta ccgcttcaac ttgcccctct tcttcaggcg cttcatcgag
    14341 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
    14401 tggattctga tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
    14461 catcgcaatt tatctcctat taaaaattca agcgcggcat caagacgaat atgcggcagc
    14521 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
    14581 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg gataaacggt gagtggcgca
    14641 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
    14701 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cccttcaaac
    14761 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
    14821 tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc gataaccggc
    14881 gaaaacaaac gccggaacaa ggtacgctgc ccgtagtgga aactttgcat cagctgcgtc
    14941 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatca
    15001 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccacc
    15061 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
    15121 tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
    15181 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
    15241 tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc cagccggttt
    15301 tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt gaccgaccat
    15361 tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa atagtcctgc
    15421 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc cagatacagc
    15481 gtggaggtgt ccttgaagtg gcgtagcagc gaatcgttcg atttaaaacg tagcgccagg
    15541 cggatttcac tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc atataactgc
    15601 gccagtcctt catcatatgt gaagcgcgga atgccaaagt cacgctgagg aatgcgtttt
    15661 acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc tccggcgtga
    15721 atattgagca actgattgac catcgcagtg ataaacgctg ttttgccgct gcggcttagt
    15781 ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attaagttca
    15841 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aatcattcgt
    15901 aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg tcatcgacgg
    15961 gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc tatgtcccct
    16021 actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg
    16081 aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa
    16141 ttacagtatc agccaaacaa gctggcccgc gcgctgacct cttcgggttt tgatgccatt
    16201 atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt ctctgaagtt
    16261 ttgcatgcca tcaccgccaa agctgaagag gaaggtttcg acgtgatctt gcagacgtcg
    16321 cacaatccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa aatgattaaa
    16381 ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact tgataaatac
    16441 gatattcctg tcgtggtgat tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc
    16501 gataccgata attttggcga cagcattgcg ttgaccgatg cattaattga aagtgggcat
    16561 caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcagtgga tcgggtaaat
    16621 ggttataagc agagcctggc tgcgcataat attgcagtgc gtgatgaatg gattgttgac
    16681 ggcggttata cccatgaaac agcactgcaa gccgcgcggg aattattaag ccagtcaccg
    16741 ttgcctgagg cagtgtttgc tactgacagc ctgaaattaa tgagtattta tcgtgcggca
    16801 gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag taatgaaacg
    16861 ctgtcgttta ttttaacgcc tgcgccaggc ggtattgata tcccaacaca ggagttaggt
    16921 caacaaagct gtgagttatt attccgctta atttctggaa aaccgtcacc acagaatatt
    16981 accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga atgccggatg
    17041 cgatgctagc gcatcttatc cggcctacat ttatagttcg gataaagtac tcgtgccgca
    17101 tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc ataatggaat
    17161 ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag cgaaacggaa
    17221 agcccgttct ggaaatcata accgtaaaat aaacctacac ggttaaaatc atggccttca
    17281 cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat gcgcgtatac
    17341 ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc ttgcgtagaa
    17401 aactcaccgt tattgcggct gtcgtccata ttaaagccgc gctcgagata atagttcact
    17461 cgcaaggcaa ccgtttcgtt gaaggtatat tgcaggcctg tttccgtttc gactcgggta
    17521 tcagcgtaac cggtcgtgtt cagatcgttg gcaaatttat acatcgacaa ccaaccgttg
    17581 aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca gcgctgcata
    17641 ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg gaaaccgccg
    17701 gtcaggccga aactgaaatc atcgttttcg aggaactgat aatgcacctc cagctccggg
    17761 cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc ctgataatag
    17821 gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg ctccgccagc
    17881 ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc gccgatatta
    17941 aagtgccagt cgttcctttc ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt
    18001 gcggtacagg gtaataactt tttcattatt gttccttgta ttattctggg ggaatgcctt
    18061 ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga catttcgtca
    18121 tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca ccagcacgga
    18181 ccactaactc gtgcccccca accgtggtgt agagcataaa ttccgcaccg gttaattctg
    18241 ccacgctaat tttggcggaa atgttatttt cctcttgcgc gtccggatga atatcttccg
    18301 gacgtattcc catcacgatg ggcttatgca aacttccctg tgttttcaga accgctaatt
    18361 tctcttcggg aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca tcgatcgtgc
    18421 cacgaataaa attcatcgcc ggtgatccaa taaatccggc aacaaacata ttcgctggtt
    18481 ggttataaac cgttttcggc gcaccaactt gctgaacaat cccatccttc ataatcacaa
    18541 tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc atggtggtat
    18601 tcagcttctg gtgcagcttg ctgatctctg cgcgcatttg cacgcgcagc ttggcatcaa
    18661 ggttagagag cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc gcccgcccga
    18721 gcgccacacg ctggcgttgc ccgccggaaa gcgcgcccgg cttgcgtttc aggtactcac
    18781 gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctct ttggcgattt
    18841 tttgcatctt caggccaaac gccatgttgt cgtaaaccgt catatgcgga tacaacgcgt
    18901 agttctggaa caccatcgct atattgcgtg ctttggctgg aacgtcattc attcgtttgc
    18961 cgtcgatcaa cagatcgccg ccgctgatct cctcaagccc ggcaatcatg cgcagggtgg
    19021 tcgacttacc gcagcccgac gggccgacaa acacgatgaa ctctttatcg gcaatttcca
    19081 ggttgaagtc cttcaccaca tgcacctggt tatcgtagat tttttgaata tgttgtaacg
    19141 aaagctgagc catgttgatt cctttgctat acgttttgcc agaaggccga taaccgcggc
    19201 caggtgagtg attccgttga aggcaacagt aattgcgccc cggttaagcc cgcgccgatc
    19261 cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc atcttcaatg
    19321 ccgatacatg cctgcggcgg cacgcccagc ccggcacagg cggcgagaaa gatttccggg
    19381 tccggtttcg agtttttaag ttgggaagca tccgcgcaga aggtgaaaaa ctcgcgcagc
    19441 tccagcgccg ctaaaatcgt cggcgcattc agggagacag aagccagccc aaccgggatc
    19501 tgctgtgcac ggagatctgc cagcaaagag cgaatgccgg gtagaacagc gctgaccgtc
    19561 aactcgcgca gtgagtggac atagagcaga tttttgcgat acgccagttg cgccctctcc
    19621 tgcgagttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag agactcatcg
    19681 cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc aatttcagcg
    19741 gcaatctgct gccacgcctg gaaatgcaga tgcgcggtat cggtgattac accatccaga
    19801 tcgaaaatta ccccttgcag tttcattcat cctcctgatg tgtggtagcg gtcccattga
    19861 agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacggat ataagctgac
    19921 cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc gcgtcaagag
    19981 tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc cactgctcag
    20041 gtaacgccgg attgagatgc aattcaccgt cacgcacgct caccccggca aaaccctgaa
    20101 tcgcccccag ccagatagcg ccagttgcgg cagcatggat accatcatca caactatgcg
    20161 gatcagcacc aagatcgatt tcagtcccct cgcgccagaa ctgataactt tgggtcagca
    20221 ggccacagcg tgcggcaaca atgccgtgga ttgctttact taatgacgag tcgtgaatag
    20281 tgcgcggttc ataaaattgc agattggcaa gacacgatgc cgctgagaac tgctccggca
    20341 gcatgtaatt gagcatcacc acatcagctt gtttgaggat ttgcatctcg ttcacttctg
    20401 cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat ttcgccagat
    20461 taatcgccgg cttagccata aacgaatcat cctgcggcaa aacgccgtcg ggctgaattt
    20521 ctggcagccg cagttcttta aggaacattt cggcgcgatg gataaacgca tcgtcgctac
    20581 agccaaactg gcgggcaata ctcagcgcct gctggacgtt gtaatacgcc atatagctgg
    20641 tgaaggcgtt attattgaca tgttcggtat attcgtcagg cccaataaca tcatgaattt
    20701 ccagacggtc gttaaccctc accgcgcggc taatccagaa ctttgcagtt tccagaagta
    20761 gcgccatgcc ttcatgcgca atgaaacttt catcccccgt ggtctgccag tattgaataa
    20821 ccgcccaggc gatatcggcc accagatgat gttccgcctg cgccgaggcc actttttgcc
    20881 gcagcccggt gcgaatgtta atggcggcaa attccggcgt ctcttcttcg ccgctgcgcg
    20941 cgctttccca cggaaatagc gcgccctgcc agccgttgcg tcgcgctttc tcctgcgcgc
    21001 ctggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga tcgctaaaca
    21061 gatgaaacgc taacaaaaat acttctgtat cccagaaaac gtggcctttg tagccttcgc
    21121 cggttaagcc ttttgccgca atgctgctgc gctcgtcgtg ggcaggcgtc atgatgcgca
    21181 gatgataaag cgcataatct aacgcttgct gatcgtgcgc ttcgccgcca tttaccgtga
    21241 tacgacgttt ctgccaccat tgacgccagt tttctgttga tactgcaaga agttggtcgt
    21301 aactctgctg cgcgcacatt tcaagctggc gaagcgacgc gctgccccac tcgtctaaaa
    21361 cggcttgcct gtcatcccgc cagtcgatcc acaccagttt ttgcaacgtc actgtctcgc
    21421 ctgcatgaag ctgcgcactg gtatgttgct gcaaacggcg ctctttggcg gtatagcatt
    21481 gctgcacatc accgctcacc atacagcaac agctgatggc cacatcactg cgtccatcct
    21541 gggtggtgta gatcccctgc atcagatgct gaccaaacac ccgcacctgg gtttcgtcga
    21601 gatgttggcg accgtggttg gtttgcgtgg cgtcgatacc tgttgaaatc agcactgacg
    21661 cgtcggcgtc cagtggcgta atagtgattt ccagcacaat gagcggcagt tggtctgccg
    21721 aaacaaagcg acggctggcg atggtgtaac ctgcgccgtt gctggtacgc cagacaacgt
    21781 tgcggcgtaa ttcgccactg gcaaagtcaa gctcacgctg ccaggcttcg tgggataacg
    21841 agaaaacctc accatttatg gcaatctcca tccccaagat atcaggcagg ttcaccagtt
    21901 cgttgatttc acctttaccc gcccgatgat acagccccgc cagatacatc cctcgcgtct
    21961 ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc ccctgcgcca
    22021 tcagcgatgc atacttgttc agggtatgct ggctaaaatg gggttctgat aacgttactg
    22081 gcctggtcat aattccacac gcgtccctgt ttctgcggat tgatataacg ccgccaccag
    22141 ttgctggatg atgtacccct gctcggcgtc ggctatcatc acgggcttgc cctgtacgtg
    22201 attgataaag gcttccatgc tgcgcaaatg gcggttgtcg tctgccattt cccgttgcat
    22261 cagcgtcatt aattcaccgt tgttatcggt gtagatatgt gctggaaaca gcgtcgcacc
    22321 ggctttatca ccacaaaagc tgacgttcat aatcgactgt tcgcggatgt tgagtgcaaa
    22381 tgacgtttcc agccacagga tgccgccgtt atgaaattca atggtgccaa acagcgaatc
    22441 ttcgacgctg taagttgccg gatcccactc gccaaattga ccacagctct tttgcgtgcc
    22501 gatcttttga aagctatgcg cattcacgct tttcaccgcc ggaaagccca gcacatacat
    22561 cgcagcatcc agcatatgaa tgccgatgtc gatcagcggg ccaccaccct gcagttcttt
    22621 attggtaaag acaccccagc cgggaacgcc gcagcgacgc agggcgcggg cggtggtaac
    22681 gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg tatcgagcgc
    22741 aaaacgatgg tgaaagtcgt aggccagcac cttgccctgt ttgcgcgcgg tatcgcacat
    22801 ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg cacatcacat ggcagcccgc
    22861 ttccagtgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga cgctaaccac
    22921 atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg cattaccgta
    22981 tttatctgcc agcgcctgcg cctgggaaag gcggctatca cagacagcca ccagttccag
    23041 atcgttgcgg gtgcagtagt acgaagcatg aaccttatcc gccacctggc ctgcgcctat
    23101 tatcgcgacc cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt
    23161 acgcaaccag gccaacgaat cacggtacgc ctgggcagga tcttctgccc ggatacgccc
    23221 ttcatacacc acataaccct gatagttatc ggcgcgcagc tgttcaaaca gcgcgtggaa
    23281 atcgagggtg ccactgcccg gctggtaacg atggttatcg gcaatatgca catgaccgag
    23341 caggtcacgg ttgtcgtgca gcgcctgcgc caggttatct tcttcgatgt tcatgtgata
    23401 gaaatcgccg ataatctgca catgtttaag attgttttcg acgatgtaac gacgagcatc
    23461 ggcgagggtg ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt
    23521 tccggtacgc gcggcgacct gttccagtac gcgcagggaa tcactcacca ttttgcggtc
    23581 gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc
    23641 tgccggaacg acgatgcctt taccccccac ttctgccagc gcttcgagaa tgcgttcgat
    23701 ctgctttaag ccattaagac gacgctcttc gataaagtca ccaatccagc cgtcatagcc
    23761 accgcaggcg gtggtcaccg gtaaaccggt ttctttgatt gctgctttga cttcttcgat
    23821 gttgttgacc agcagtttgc cgtcaatctc aaaaccatcg aagcccatct ctttgatata
    23881 acgaaatttc tccagaatgt tttccggaaa gaatgcctga ttctgtgtgc ctattttcat
    23941 tttttcattc ctttgaatta aaacgtaacg cccattttga tgctctgttc cggatgctga
    24001 tcgacatact gcatgtagct ttccgggctc ttggcaaagg tcactaccgg gtcgattaaa
    24061 tcttcgcaat tgagataacc gttcatcagc agttcccagc aggtttcttc aatacgctta
    24121 cggctccagc gcggataatc cgggttcggt tcgctgcacg cacgggagaa aacaattttg
    24181 gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac
    24241 gcgacataag agatggtgcc gccataagca aggccacgca gcgccgattg cagcgcgtcg
    24301 gcgtaaccgc tggtttcgat aatcacatcg gcaccctgct tgccggtcag cgttttgatc
    24361 tcttttccta cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca
    24421 atatcacagc gataggcgat aggatcgacg ccaatcacca ccgacgcgcc agcgcgtttc
    24481 gccagttgga tggctatttg accaatcgcg ccaagcccta ccaccaccac aaaatcaccc
    24541 acgcgcacgt tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacaaacg
    24601 gcgtttttcc acgagctgcc ttccggcatt ttgcgcagct tgtagttatt caccgcgtta
    24661 atgatgaccg tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtcg
    24721 gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg
    24781 ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcggag tgaacatctg ccattcgccg
    24841 ttaaagtctt catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt
    24901 ttcggtgcgc cgaaacgcac gcggattttc acttcattgg ctaaaatcgc ccgatcttca
    24961 tactcaacca gcgcagcaac acgcggtgct gtggctacta actttttcat ttaaaactcc
    25021 ttgtaaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata
    25081 aaacgttccg aaagcgcata cataatcacc accggcaatg cggtcaccag tgaggccgcc
    25141 atcatgcgtc cccagatgta atctggcgta ctgaacagcg cgttcaggcc caccggtaag
    25201 gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgccacc
    25261 ataaagcagt agacgaacac ggatatcagc ccggacatcg ccaacggcac ggtgatgcgg
    25321 aagataattt gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcgggg
    25381 atggtgtcga agtagctttt cagcataaac acggcggtgg gcagagtttg cgtcaccatg
    25441 gtgatgatca gcgccatctc ggtgtcgtaa atgcccagcg cggtgatgat tttgaacagc
    25501 ggcaccacca gcaaaatgcc agagaacata tacaccgtgt aaaagctggc attgatggtc
    25561 atccgacctt taaagcgcag gcgggaaagc gcgtaagccc caagaatgcc gaggaatacc
    25621 gccaccacgg atgaaaccac cgacaccacc agactgttac ggaagtagtc gacaaacggg
    25681 aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg tccactgctg cggcagcagc
    25741 gtaggatgca gtgagatcgc ctctttcgcg cccttgaacg aggtcatcag catcacaaaa
    25801 aatggaaaca gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatgcgactg
    25861 agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc
    25921 gaaaataatg atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc
    25981 aaaggcggtt ttgtagagat atacaccgag aatatcgacc ttggtcgtca gcaaataaac
    26041 atccgcgaac atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaggaccgg
    26101 cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag gcattagcgc catccatctc
    26161 tgcagcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc
    26221 atacgggaag tagcgccaga tggcgaacag caccaccagc acaaagctac tgcccggatt
    26281 atcgaaccac agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac
    26341 aatgccgtag ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt
    26401 tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa
    26461 aaacatcgcc actgccagcc cgagcacggt gctgcccgcc accaccagcg cggtatacca
    26521 gaccgtcatc cacagcgagt gccagaagcc gggatcggag aggatacgca catagttact
    26581 caccccaaca aaggttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc
    26641 gatattcgac accattggcc aggccaccag accacccagc agtaataagc tgggcgcgag
    26701 aagcagcagc gcaaagggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc
    26761 ctgtcctggg tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc
    26821 gtgccaggga gatcggcttt acccacggtg acgttatgca ccatggagct caccacgcca
    26881 gaacccgtca catcacccat gcgggtaaag tttttatccc ctactgcacc aaagacctga
    26941 atatttggca gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg
    27001 ttgtctttcc aggtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc
    27061 gacatcatca cccagtcggc aatgttgtct gcctgctcca taaaggtgac aaatttttca
    27121 gctgcttccg tctcttcggt cttttgcccg gcggtaatgg tcagcgaggt caacatgccg
    27181 tagaccgcag agtttttctc ggttggcacc acgaaaccga cgtttttcgg gtcgccttct
    27241 ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt gccgttcata
    27301 aaggcgtctt tcacttccat gatgtcgtta gaacccggca tagtgttggc agcaaggtcg
    27361 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
    27421 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
    27481 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
    27541 gcaacgtcca gcagctgttg ccagtttttc ggctcctcaa gccccgcttt tgccagcaca
    27601 tctttgcgat accagatacc gccaatccag gcgctgacag gaacaccggt ccatgcgcta
    27661 ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg
    27721 ctgatgactg tggcaaccgc tttgcgatcg ataagctgct ctttgtccat cactttggcg
    27781 tagtcatggc tggtttctat cacttccggc agcgaaccgc tacgtgaaag agtaatgact
    27841 ttagtgttat aggcatcttc ttccacgggc acttgcttaa cggtgatgcc agggttttct
    27901 ttttcaaaac gggcaatcaa tttactgata acggcctggc gctcctgctc caccgaagaa
    27961 tgcataaatt cgatggatac attcgtttta ttttcttctt tacagcctga aatcagggcg
    28021 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
    28081 tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccaata tatcaacttc
    28141 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
    28201 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
    28261 tgaattaatc gtatcaatgg taaaattatt atccggatga aactcgttat ggctgcgacg
    28321 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
    28381 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    28441 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    28501 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    28561 ttcatcgcta ctctcacggc ggcttaacgc atccatatag gtcacattta tttcatacgg
    28621 actgcgtgta ccgtcgggat tatttttcca gtttactaat gcaccttcct gctgtaacgc
    28681 ctcgaccagc tctaatattt cgctttcagg caacaagccc cgtagcgggt ttagcccgat
    28741 gccatcgtga gaggcgagga agttaaacca ggtggtgttg ctggaaggta gtgtcaggtt
    28801 ttgcgcccac gcacaaagcg cctcaacgtt ctgtttttgc accgcatgca gcaccagtgg
    28861 cggcagcgag aactggtaca ccatatgtgc ttcgtcatcg cctgcgccaa agtaagcaat
    28921 gttgtcttta tgcggaacat tggtctcggt aatgatcact gtacctggcg caacgttatc
    28981 aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga tgcagcttgt
    29041 tcccggctct ttccacataa agccaacggc atccaggcgg acatactcag cacctttcgc
    29101 aaggtaacag agtaaaacat ccaccatcgc cagcaacact tcagggctac ggtaattcag
    29161 gtcaatttga tcgtcactaa aggtggtcca taaatggcgc gttgaatgat cgcgcatctg
    29221 gaatggcgtt aataacggta acgcacgcgg gcgagtgacg gcgctgagat cggtttgcgg
    29281 gtcaacggca ataaaaaaat cttcaaaacc tggatgctgt tgtaaatagt ttttaaacca
    29341 ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc
    29401 gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac
    29461 cgaaaagcca tcatcagatg accatggata aaacggcaac aaatgaacat gtgaaaaaat
    29521 gctttgcagc cattgatggt aaaactgatt aaatgtgggt aatggtttta aatcattgct
    29581 gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg
    29641 tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt
    29701 tgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct gtttcatgtt
    29761 gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcagaaaa taaactgtca
    29821 attgagcaca agggttttag tcacgcagaa aatcattttt taccagggaa aaagcgtatt
    29881 tgtgagctga cgcaaataat ctatggggat gtaaataagg tgtgacagaa aaaaaaacgg
    29941 cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct
    30001 ttcaggccac cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgct
    30061 tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg
    30121 gcggtggcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc
    30181 tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc
    30241 agtgaaaaca ccagtgccag agcaagtaag ccttttttaa acatgtaagc gtccttctat
    30301 ggatgaaaat tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc
    30361 accttttata acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca
    30421 cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag cccgccacac
    30481 ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccag
    30541 gctttacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg
    30601 gaaacggcta cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg
    30661 cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggaaggtag
    30721 ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta
    30781 agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac
    30841 cagttttacc ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc
    30901 ctgctgtgga atacgccata attttttatt gagattaatg cccgccatta gcgatccctc
    30961 cagttcggat gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt
    31021 tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga
    31081 ccagaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg
    31141 gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc
    31201 gcgttcggac gccgcctgga tgttattatt gattgtcttg cttcattttg gcttttaatt
    31261 gtgccagttg ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg
    31321 atttttgttt accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt
    31381 cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca
    31441 catcgcgcga cgagtttgcc gcctgatgac gtaacatcaa tgcctgctgg cgagcgcgtg
    31501 tttcgctcaa tttgttttcc agctcaccga tctctttctt catgcgtgcc agcgtgtcgt
    31561 ccaccagcgt cacttcatgt tccaggctct taatcagatc ggtcagtttc tgtttttcaa
    31621 ttaacgctgc acgtgccaga tcctctctct ctttcagcag cgccagttcg gctttttcct
    31681 gccattcaac ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg
    31741 ccaacgcgcg cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca
    31801 tcagacgaac cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat
    31861 tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt
    31921 caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt
    31981 tataaagatc tgattgaaga atcaacagca acatgccagg atgagttagc gaattacact
    32041 aacaagtggc gaatttcatc atggcagaat acaaagataa tttacttggt gaggcgaaca
    32101 gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca
    32161 tcatcggcga acgcggcacc ggtaaagagc tgattgccag ccgcctgcat tatctctcct
    32221 cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg
    32281 attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatcctg
    32341 ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga
    32401 tgatggtgca ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg
    32461 gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg
    32521 cgatggtcaa tgaaggcact tttcgcgctg acctgctcga ccgactggct tttgatgttg
    32581 tacaactgcc accactgcgc gagcgcgaaa gcgacataat gttgatggca gaacactttg
    32641 ccatccagat gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca
    32701 gagaaacatt gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg
    32761 aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc
    32821 cctttaaacg gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa
    32881 cactgccgct ggatttacgt gagtttcaga tgcagcagga aaaagagttg ctgcaactca
    32941 gtttgcaaca agggaaatat aaccagaagc gcgcggctga attactgggg ttaacttatc
    33001 atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg
    33061 caggatgcgg cgtgaatgct ttattcggca tacataatca atacgtcgtt tgcaggcctg
    33121 ataagcgcag cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca
    33181 aaattttcat tgccgcatcg aattgcacat ccgggatggt cagcggatac aggaagcgaa
    33241 tcacgttgcc gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct
    33301 ggattttctg tgcaatcgcc gctgacggct tgcccgtttg cggatcgtta aactctgccg
    33361 caatcatcga ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa
    33421 tcaacgtgtt tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt
    33481 cgataatatt gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc
    33541 cacccaaccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa
    33601 gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat
    33661 gatccatggc aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca
    33721 tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa
    33781 cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt
    33841 cgatgtctga tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt
    33901 gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt
    33961 tgtacggcgc aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccac
    34021 cgctaaacgc aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt
    34081 ccaccgcttc cgcaccggta gtgaagaatg cggttttagc ctgcccgctc actggggcaa
    34141 gggcgttaat tttctccgcc agcgtgacgt agctttcgta cggcacaatc tgatacgccg
    34201 tgtgggtaaa ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc
    34261 cggtattcag caccgcaatg cctgcggcga aatcgatgta ttcgttgccc tctacgtcct
    34321 tcagcgtagc gttttctgcc gactgggcga agaagttaca catcacgcca accccgcgtg
    34381 gggtagcaga aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac
    34441 tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ataggctgcg
    34501 cccatcgcgg taaacggtac tcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga
    34561 ttggcgaagg catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat cagacgcccg
    34621 gcaagatggg tacaggtgac gccgtggccg ctgtagccct gcatgtaata gatgttggta
    34681 tcgaggcgac cgaactgcgg catccgtgaa agggtcagca ggaagttgcc cgtccagcgg
    34741 tagtcgattt tgacgccctt cagttgcggg aaggttttca gcagtttcgg caccacaaga
    34801 cgctcaacgt catccgggtc gcgcgcgccg tagaccacgc cgccgccgta cagcaaacgg
    34861 ttgtcggcgg taagacggta gtaatcaagc agataattac agtcttccac acagtagttt
    34921 ttcgggatca gcgaacgggc taaatcttcc gacagccgtt cggtggtgat cacctgcgtg
    34981 ccgcacggca tgctgcgttt cgccagttcc ggctctactt tatcgcccag atacgcattc
    35041 ccggcgacaa tcacatactt cgccgtcacc tgacctttgg cagtgcgcac caccgctggc
    35101 gtggtgtgct ggatctgcgt cacggcagaa agctcataca cgcgcccgcc gttcaggcgg
    35161 atggcatcgg cttcaccaat ggcgaggttt agcgggtgaa tatgcccacc gctgtgatcc
    35221 aacagtgcgc cagtgtagcg atcgctggcg acctcgcggc gaatggcgtt ggcgtccagc
    35281 agctccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc gagtgtggcg
    35341 agctgtttat cattcatcgc cacaaacagg ccgccggggc ggtagtcgca gtcaatctga
    35401 tagcgtttga tacgttcgcg gatgatctcg ccaccctcga acatcatgct gccgagcata
    35461 cgggcggtgt ccatgccgta gcttttttcg atcacgtcga tgtcgcggct gtaggagttc
    35521 acaagctgcc cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc
    35581 actacgtcaa agcccgcttc cgccagatgc agcgcggagg agagcccggt atagccgccg
    35641 ccaaccacgc aaacgtcgca ggtgattgac tcattcagtg tgtcgaatgg tgcatattta
    35701 ttcgcactgg cggcgtagta actgctggta tgttcggtca tttcaggcct ccaggcttat
    35761 ccagatggtt ttcagttcag tgaatttttc aagggcatgc agggatttgt cgcgaccgtt
    35821 gccgctctgc ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac
    35881 gaagacggaa ccggctttca ggcgacggct catgcggtgc gcgcgggaga ggtcgcgcgt
    35941 ccataccgcc gcgccgaggc cgtactggct gtcgttagca agctgaagcg cctgatcttc
    36001 tgatgtgaaa cgcgtgacca ccaacaccgg gccgaaaatc tcttcgcggc ttaaggacgc
    36061 attcgggtct acatccacaa agatggtcgg gccgatggcg gcagccagct cggcgttacg
    36121 gccatccagc agcagttgcc ctttgctttc gccttcacga ataaagctat gcaccgagtc
    36181 ggcgtgggcg cagtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatgacccgg
    36241 ctgccagttt tgcgcctgct gttttaacag ggctaagaat tcatcggcaa tgctctcttc
    36301 cagtaacaaa cgcgttccgg cgatgcacac ctggccctgg ttgtagaaga tgcctgcggc
    36361 ggtggcgctt gccgcttttt gcagatccgg acaatcggcg aaaacgatgt tggcgctttt
    36421 gccgcccgct tccagccaga cacgtttcat gttgctatca cccgcgtctt tcagcagttg
    36481 tttacccgta cgcgtggagc cggtaaaggc gatggcatcg atatcgttat gacgcgaaag
    36541 cgcctgcccc gcttcatgac caaaacccgt caccacgttc aacacaccat ccggcaagcc
    36601 tgcttctttc gccagccccg cgagacgaat cgcactgagc ggtgattttt cagacggttt
    36661 tagaatcacg ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag tcagcaacag
    36721 cgggaagttc cacggcacga tggcggcaat cacgccgacc ggttcacgca cgatcatcgc
    36781 cagctcatgg ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta
    36841 ccagcgaatg gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt
    36901 gccggtgtcg agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggc
    36961 gagtttattc agtaccgctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc
    37021 aaatacgccg cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat
    37081 tttcgccagc ggtgcctggg tgaccggatc aacggtttca aaggtttcat tttccgccgc
    37141 agcagtatat tcaccgttaa taaataagcg gttttcaatg gcgagactta acgctttatc
    37201 ctgccagtaa gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat
    37261 atgcagtttg tgaacgtcag ggcgcagaga tccccgggcc gcatgcccgt ttatttctgg
    37321 cgacattctt tatatacact tcatccttca agccgcctct gccttggctg cgtttgctcc
    37381 cccgaatcac ttacctgagt aagctcatcg ggattcgctc acttgccgcc ttgatgcaac
    37441 ttgaatgatt ttgtgtatag attgccgggc gcattgcgct tacccggctt acagaacaag
    37501 aagattaaaa cgtggtgggc gtatgggcgc tgataattcg gcaaatacct gccgacgtat
    37561 tactgaaact gtgcgggatg ccggtattaa tggcataact ttgccccgcg acgagatgat
    37621 aatcctgacc attaatcgtc agaacaattt caccttccag tacagtgcct atttcctcac
    37681 cctgatgctt aattctttcc ccagtggttg tgcccggctg gtacgtttca aagatcatcg
    37741 ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat tgacacaccc tgactgccca
    37801 tctcaattaa gtcgtcctga ttaatgacga cctgcggctc atcaggtttt tccggctcgg
    37861 aaaagaattc cgagagtgac agaccataca ccttcagcag cttttgcagc gtactgatgg
    37921 cagggctgac tttatcttgt tctatcgtac tgatagcact gtgagtcagc ccggagagtt
    37981 cggcggcacg acgttgtgaa agcccctgct gctggcggat ttccgacaag cgttttcctg
    38041 gcgccagtcc ctcatcactc atatttgcat ttccttaaac tgtagtggtc agagtcgctg
    38101 tttttcagcg atatggtgct gacaagcggt gataaagccc tcgaacaata tacgcgaaag
    38161 cgcgtactcg ctactgttcc attccgggtg ccactgtacg cccagcgcaa aaggatgatt
    38221 gatgacgcta accgcctcaa ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg
    38281 gctaacgacc ttcgcccctt gcccatgcag agagtttacc caaaagttgc tacattcagg
    38341 taacaacgca gacagtaatc ccccctcttc aacctgaact tcatgcgacg gtgcatattg
    38401 ctgttccacc ggcagttcgg gatcttcccg atgttccagc aattcaggct gttcgcacag
    38461 cttgcgatgc aacgaaccac cggttgccac caccagttcc tgtaaacccc ggcagatggc
    38521 gaaaatgggg atgcgccttt cgagtgcggc attaattaac gccatgctca gaagatcacg
    38581 cccgggatcg gcgtcaggct catcgccgtt ttcaccatat aggtgcggct gcacattgct
    38641 gggactacca ggaagataaa tgccatcgag tttcggcaaa agttgttcaa gtaatgacgg
    38701 ttccgccagc gcatgtggta gcgcaattgg caagccgcct gcgtggatga tggcattcag
    38761 gtacttttct tgcagagtct gggtcgcatg acccttaagc ctgttcctgc acattacgac
    38821 accgataacc ggattgttca ttatattttc catgctcaat ctcacaaagt ggactaaatt
    38881 atcgccatta ctgcggttta aatgaccttt atgttcaata ttttttcaat ctagcagtgg
    38941 gttttcattt ttgcaaactc aatttaacat ttgacaaaca tttagtttgc atacagattc
    39001 gaatggtggt cattatattt tacgctttga taacgagcgg aaaacaaacc aaaggcgaag
    39061 aatcatggaa accaatatcg ttgaagtaga gaactttgtt cagcagtcag aagagaggcg
    39121 gggtagcgcc tttacgcagg aagtgaaacg ctacctggag cgctacccga atacgcaata
    39181 tgttgatgtt ttacttactg atttgaatgg ttgcttccgt ggaaaacgta tccctgtttc
    39241 aagcctgaag aagttggaga aagggtgtta tttcccggcc tcggtgtttg caatggatat
    39301 tctgggcaac gtagtagaag aagcgggtct gggtcaggaa atgggcgagc cggatcgtac
    39361 ttgtgttcct gttctcggtt ccttaacccc ttctgccgcc gatccagagt ttatcggtca
    39421 gatgctcctg accatggtcg atgaagatgg cgctcccttt gacgttgagc cgcggaacgt
    39481 tcttaaccgc ctgtggcagc agctgcgcca gcgcggattg ttcccggtcg tagcggtaga
    39541 gctggagttc tatttactgg atcgccagcg cgacgctgaa gggtatctgc aaccgccctg
    39601 cgcgcccggc accgatgacc gcaatacaca aagccaggtt tactccgttg ataacctcaa
    39661 ccacttcgcc gacgtgctca atgatattga tgaactggcg cagttacagt taattcccgc
    39721 cgacggtgcg gtcgccgaag cctcgccggg tcagtttgaa atcaaccttt accataccga
    39781 taacgtgctg gaagcctgcg atgatgcgct ggcgctaaaa cgcctggtgc gtctgatggc
    39841 agaaaagcat aagatgcacg ccacttttat ggcgaagccg tatgaagagc acgcgggcag
    39901 cggaatgcat atccatatca gtatgcaaaa taatcgtggc gagaatgtgc tttctgacac
    39961 agaaggcgaa gattcgccgc tgctgaaaaa gatgctcgcc gggatgattg acctgatgcc
    40021 gtcgtcgatg gcgttgctgg caccaaacgt gaactcgtat cgccgcttcc agccgggaat
    40081 gtatgtgccg acgcaggcgt cgtggggcca taacaaccgc accgtcgccc tgcgtattcc
    40141 gtgcggcgac cgtcataatc accgcgtgga atatcgcgtg gcgggtgccg atgccaaccc
    40201 atatctggtg atggcagcga tttttgccgg tattttgcat ggccttgata acgagctgcc
    40261 gttgcaggaa gaagtcgaag gcaacgggct ggaacaggaa ggcttaccct tcccgattcg
    40321 ccagagcgat gccctgggtg agtttatcga gaatgatcac ctgcgccgct atttaggcga
    40381 acggttttgc catgtgtatc acgcctgtaa aaatgatgag ctgttgcagt ttgagcgcct
    40441 catcacagaa acagaaattg agtggatgtt gaaaaacgcg taattcccca tgcggggttg
    40501 tttcatcggc ctgagcggag ccaggcctga catttgctgg taatccagac atttcccgga
    40561 tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta acgattaaga ctgtgacgag
    40621 gaaatgagat gatgccgatg attaaatccc cgcatggcga gggaggctgc gtatgcgccc
    40681 ctcctgctac tgactggact ccgccccctt tgcttccctt gctaaacagg tttgatttca
    40741 ggttgacacg accgcaaacc ttattacgca ggggaggcag caattatggc tattaattca
    40801 ccactgaata ttgctgcgca acccggcaaa acccgtctgc gaaaatcact gaaattgtgg
    40861 caggtggtaa tgatgggtct ggcctatctc acgccgatga ccgtatttga tacttttggt
    40921 attgtctccg gcattagcga cggtcacgtt ccggcgtcct atttgctggc gctggcgggc
    40981 gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc agtttccgga ggccggttcg
    41041 gcctatacct acgcgcaaaa gtcgattaat ccgcacgtcg gatttatggt cggctggtca
    41101 tcactgctgg attatctctt tttgccaatg atcaacgttt tgttggcgaa aatctatctc
    41161 tccgccctct tcccggaagt gccgccgtgg gtgtgggtgg taaccttcgt cgccatttta
    41221 accgccgcga atctgaagag cgtcaacctg gtcgctaact tcaataccct gtttgtactg
    41281 gtgcaaatct ccatcatggt ggtgtttatc ttcctggtgg ttcagggact gcataaagga
    41341 gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca gcgagaacgc gcacctgatc
    41401 ccgattatta ccggtgcgac gattgtctgt ttctcgttcc tcggtttcga tgcggtgacc
    41461 acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc cgaaagccat cttcctgacg
    41521 gcggtatacg gcggcgttat ctttatcgcg gcgtcgttct ttatgcagct gttctttccc
    41581 gatatcagcc gctttaaaga cccggacgcc gcactgcctg aaattgcgct gtacgtcggc
    41641 ggcaagctct ttcagtcgat tttcctctgc accacgtttg tgaacacatt agcgtctggc
    41701 ctggcttcac atgccagcgt gtcgcgtctg ctgtatgtga tggggcgtga caatgtgttt
    41761 ccggagcgcg tgtttggcta tgtgcaccca aaatggcgga ctccggcact caacgtcatt
    41821 atggtcggga ttgtcgcgct gtcggcgctg ttctttgatt tagtcaccgc gacagcactg
    41881 attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc tgtcggtgtt taatcatttc
    41941 tggcggcgta aaggaatgaa taaaagctgg aaggatcact tccactattt gctgatgccg
    42001 ctggttggcg cgctgacggt gggtgtgctg tgggttaacc tagagtcaac gtcactgaca
    42061 ctcggtctgg tatgggcttc gttaggcggc gcatatttgt ggtatctgat ccgccgctat
    42121 cgcaaagtgc cgctgtacga aggtgacaga acgccggtga gcgaaacgta atagccggat
    42181 gcgcggcgca ttatcctgcc tgagggtaac gcgccgcgcc attaactata aaaagatgta
    42241 tgacccggat cacgagtgct atcatccgcc cttatgctta gcgcattaac cccaggggca
    42301 aataatgaac cacgatatcc cgctaaaata ttttgatatt gccgatgagt acgcgaccga
    42361 atgcgcagag cctgtcgcag aggctgaacg cacaccgctg gcccactact tccagttact
    42421 gctcacccgt ttgatgaaca atgaagagat cagcgaagaa gcccaacatg aaatggctgc
    42481 tgaagcgggg attaatcccg tacgcattga tgagatcgca gagtttttga atcaatgggg
    42541 taatgagtaa ttatttaatt gcagacgatc ggtcagtatc gttgttcgga taaagcgttc
    42601 acgccgcatc cggctaacaa tgacacgcac tgcagcgtgc tttaatgggt ttctcacgat
    42661 cgtaccgctc tgctcctcgc taagttgttc tgtgagttat agttatagag aaatcacggg
    42721 tacgaatgtt gaacagatgg cgacgccacc tgttcaacga ccaaccacac ggccctcatt
    42781 acgacccctg aacacaaacg gtaatggcaa atgtcattaa atattagtaa gatagcctct
    42841 ttacataaaa tcccttaata ttatgcgcca ggtattatcg tctcttttgg tgattgctgg
    42901 acttgtgagt ggtcaggcaa tcgccgcgcc tgaatctccc ccgcatgctg atatccgcga
    42961 cagcggtttt gtctattgcg tcagcgggca agtcaacacc tttaacccat ccaaagcgag
    43021 cagtgggtta attgtcgata cccttgccgc ccagttttat gatcgactgc tggatgtcga
    43081 tccctatacc tatcgcctga tgccggaact tgccgaaagc tgggaagtac tcgacaacgg
    43141 cgcgacctat cgcttccacc tgcgtcgcga tgttccgttt caaaaaaccg actggtttac
    43201 tcccactcgt aaaatgaatg ccgacgatgt ggtgtttacc ttccagcgaa tttttgaccg
    43261 caacaacccg tggcataacg tcaacggcag caacttcccc tacttcgaca gcctgcaatt
    43321 tgccgataac gtgaaaagcg tccgcaaact ggataatcat accgttgagt tccgactcgc
    43381 ccagccggat gcttcttttt tgtggcacct cgcaacccat tatgcttcgg tcatgtcggc
    43441 agaatatgcc cggaagttag agaaagaaga tcgccaggag caactcgacc gtcaaccggt
    43501 cggcaccggt ccgtatcagt tgtcggaata ccgcgccggg caatttattc gcctacaacg
    43561 tcatgatgac ttctggcgcg gtaaaccgtt aatgccgcag gtagtggtgg atttaggctc
    43621 cggcggcacc ggacgtctgt cgaaactcct gaccggggaa tgcgacgttc tggcctggcc
    43681 tgctgccagc cagctatcca ttttgcgtga cgacccgcgc ttgcgtttaa cgctgcgtcc
    43741 tgggatgaac gtcgcctatc tggcatttaa caccgccaaa ccgccgctaa ataatcccgc
    43801 tgtccgccat gcgctggcac tggcgattaa taaccagcgc ctgatgcaat ccatctatta
    43861 tggtacggct gaaacggcgg cctctatttt accgcgcgcc tcgtgggcct atgacaacga
    43921 ggctaaaatt actgaataca atccggcgaa atcgcgcgaa cagttgaagt cgttggggct
    43981 ggaaaattta acgctgaaac tgtgggtgcc cacacgttcg caggcgtgga accccagtcc
    44041 actgaaaact gccgaactga ttcaggcgga tatggcgcag gttggcgtaa aagtggtgat
    44101 tgtgccggta gaaggtcgct ttcaggaggc gcggttgatg gatatgagcc atgatctgac
    44161 gttatccggt tgggcgacgg acagtaacga cccggacagt ttcttccgtc cgttactgag
    44221 ctgcgcggca attcattcgc agaccaacct cgcccactgg tgcgatccga aattcgacag
    44281 cgtattgcgt aaggcgctct cttcgcagca gctggcggcg cgtattgaag cctatgacga
    44341 agcgcaaagt attctggcgc aggaattgcc cattttgccg ctggcgtcgt cattgcgttt
    44401 gcaggcctat cggtacgata tcaaaggtct ggtacttagc ccgtttggta acgcctcctt
    44461 tgctggggtg tatcgcgaga aacaggatga ggtgaaaaaa ccatgattat cttcacctta
    44521 cgccgcattt tgttattgat tgtcaccttg ttcctgctga ccttcgttgg cttcagcttg
    44581 agctatttca cccctcatgc gccgttacaa ggcgcgtcac tgtggaatgc ctgggtgttc
    44641 tggtttaacg gcctgatcca ctgggatttt ggtgtttcca gcattaacgg tcaaccgatt
    44701 gccgaacagt taaaagaggt cttcccggcg acgatggaac tgtgcatcct cgccttcggt
    44761 tttgcgctga ttgttggcat ccccgtgggg atgattgccg ggattacgcg ccataagtgg
    44821 caggataact tgatcaacgc catcgcctta ctgggctttt caatcccggt gttctggctg
    44881 gcgcttctgt tgacgctgtt ttgttcactc acgctaggtt ggttgcccgt ttcagggcgt
    44941 ttcgatctgc tctacgaagt gaaaccgatt accggttttg cgttgattga tgcctggctt
    45001 tcggactcac catggcggga tgaaatgatc atgagcgcaa tccgccatat gatattgccc
    45061 gtgatcaccc tgtcggttgc gccaacaact gaagtgatcc gcctgatgcg tatcagcact
    45121 atcgaagtgt atgaccagaa ctatgtaaaa gcggcggcga cccgcggttt gtcgcgcttt
    45181 actattttgc gtcgccacgt tctgcataac gcgttaccgc cggttattcc tcgcctgggc
    45241 ttacagtttt ccaccatgct gacgctggcg atgatcaccg aaatggtttt tagctggccg
    45301 ggtttagggc gctggttaat taacgccatc cgccagcagg attacgcagc catttccgcc
    45361 ggagtgatgg tgtgtggctc actggtcatt attgttaacg tgatttctga tattttgggt
    45421 gccatggcta accctctgaa acataaggaa tggtatgcct tacgatagcg tatacagcga
    45481 aaagcgcccg ccgggcacgc tgcgtaccgc ctggcgcaaa ttttatagtg acgcctctgc
    45541 gatggtcggc ctgtacggct gcgcggggct ggctgtactg tgtatttttg gcggctggtt
    45601 tgcgccttac ggcatcgacc agcaatttct cggttatcaa ttattgccgc catcatggtc
    45661 gcgctatggc gaagtttctt tcttcctggg gactgacgac ctcggacgcg atgttttaag
    45721 ccgtttactg agcggagctg cgccaaccgt gggtggcgca tttgtggtga cgcttgccgc
    45781 gacgatctgt ggcctggtac tgggcacctt cgccggggcg acgcacggcc tacgctcagc
    45841 ggtgcttaac catattctgg ataccttact ggcgatccct tcgctgctgc tggcaattat
    45901 cgttgtagcg tttgccggac cgagtttgtc tcacgccatg tttgctgtct ggctggcatt
    45961 gctgccgcgt atggtacgtt cgatttacag catggtgcat gacgaactgg aaaaagagta
    46021 cgttatcgcc gcccgtctgg atggcgcatc aacgctgaat attctctggt ttgctgtgat
    46081 gcctaacatc accgccgggc tggtcaccga gatcacccgc gcactgtcga tggcaattct
    46141 cgatatcgcc gcgctgggct ttctcgatct cggcgcacaa ctcccctcgc ctgaatgggg
    46201 agcgatgctc ggtgatgcgc tggaactgat ttatgtcgcc ccgtggactg tcatgctgcc
    46261 aggtgcggca attatgatta gcgtgttgtt ggttaacctc cttggcgatg gcgttcgtcg
    46321 tgcgattatt gcgggggtgg aataatgcca ttactcgata ttcgtaacct gaccattgaa
    46381 tttaaaaccg gtgatgagtg ggttaaagcc gtcgaccggg taagtatgac cttaaccgaa
    46441 ggtgaaatcc gcggtcttgt tggtgaatcc ggttccggca agagtttgat tgcgaaagcg
    46501 atttgtgggg tgaataaaga taactggcgt gttactgctg accgtatgcg ttttgatgat
    46561 atcgatttgc tgcgtctctc cgcacgcgaa cggcgcaaac tggttggcca taacgtgtcg
    46621 atgattttcc aggaaccgca gtcgtgtctt gacccttcag aacgtgtggg tcgccagttg
    46681 atgcaaaaca tcccagcctg gacctataaa ggccgttggt ggcagcgttt tggctggcgc
    46741 aaacgccgtg ccattgaact gctgcaccgc gtggggatta aagatcacaa agatgcgatg
    46801 cgcagttttc cctatgagtt gaccgaaggt gaatgtcaga aagtgatgat agccattgca
    46861 ctggcgaatc aaccgcgtct gctgattgct gacgaaccga ccaactcaat ggagccaaca
    46921 acccaggcgc aaatctttcg cctgctgacg cgtctcaacc aaaacagcaa taccactatt
    46981 ttgcttatca gccatgactt acaaatgctt agccaatggg cggataaaat taacgtgctt
    47041 tactgcggtc aaacagtgga aaccgcgccg agtaaggagt tggtgacgat gccacatcat
    47101 ccttataccc aggcgctgat ccgcgcgata ccagacttcg gcagcgcgat gccgcataaa
    47161 agtcgcctca atacgctgcc cggcgctatc ccactgctgg aacagttacc gattggttgt
    47221 cgtctggggc cacgttgccc gtatgcacaa cgagaatgca ttgtgacgcc acgtttgacg
    47281 ggggcgaaaa atcatctcta tgcctgtcat ttcccgctga acatggagaa agagtgagat
    47341 gatcgaaacg ctgcttgaag tgcgtaattt aagtaaaacg ttccgctacc ggaccggctg
    47401 gtttcgtcgt cagaccgtag aagcggtaaa acccttgagc tttacgctac gtgaaggcca
    47461 gacactggcg attattggcg agaatggttc gggtaaatcc acgctggcaa aaatgctggc
    47521 gggaatgata gagcctacca gcggcgagtt attgattgac gatcatccac tgcattttgg
    47581 cgattattcc ttccgcagtc agcgtattcg catgattttt caggatcctt cgacctcgtt
    47641 gaatccccgt caacgtatct cgcaaattct ggattttcca ctgcgcctga acaccgacct
    47701 ggaaccggaa cagcgtcgca aacagattat tgaaacgatg cgtatggttg ggctactacc
    47761 ggatcacgtc agttactatc cgcatatgtt ggcacccggg caaaaacagc gtctgggtct
    47821 ggcgcgcgcg ctgatattgc gcccaaaagt cattattgcc gatgaagccc tcgcttcact
    47881 ggatatgtcg atgcgttcgc agttgattaa tctgatgctg gaattacagg aaaaacaggg
    47941 catttcgtat atttatgtta cccagcatat cggaatgatg aagcacatta gcgaccaggt
    48001 gctggtgatg catcagggcg aggttgtcga gcgaggcagc accgcggatg tgctggcatc
    48061 gccgctgcat gaactcacca aacggctgat tgccgggcac tttggcgagg cattgacggc
    48121 ggatgcgtgg cgtaaagatc gctaattttc tgaatcctcc ttccagatta ttcacctcat
    48181 tctgacaaga gattaaatct tgtcagaatg aggtgatgca aaaaataaaa tcaaatgcgc
    48241 gtgatttaag acgcaatctc actttgcagg aacgaaagct ctggcgatat cttcgcagcc
    48301 gacgttttgg tgatttcaaa tttcgccgtc aacatccggt ggggagctac attctcgatt
    48361 ttgcttgctg ctcggcgcgt gtagtcgttg agctggatgg tgggcagcat gatttggcag
    48421 ttgcctatga ttccaggcgc actagctggc ttgagtcgca gggctggacc gtgctgcgtt
    48481 tctggaataa cgagattgat tgtaatgagg agacggtgct ggagaatatt ctgcaggaac
    48541 tgaaccgccg gtcaccctct ccctgaaaga gcgagggggc cgaccgagcc gaatagctgt
    48601 tgtggtgaaa atatggtgac agttctggag aatattcggc aaggtctgaa ccgtcccagc
    48661 catcgcccta aaagggttat ggggctgtat aagcctgttt gttgctgggg tgacaatatt
    48721 tgcacaatac ggtcccctcg cccctctgtg gagaggatta gggtgaggga aaaagcgctc
    48781 accctgcccg cgccctgctc cggtcggacc tggcaactat agccacccac agccaggttg
    48841 attataataa ccgtttatct gttcgtactg tttactaaaa cgacgaatcg cctgattttc
    48901 aggcacaaca agcatcaaca ataaggatta aagctatggg ttttctttcc ggtaagcgca
    48961 ttctggtaac cggtgttgcc agcaaactgt ccatcgccta cggtatcgct caggcgatgc
    49021 accgcgaagg agctgaactg gcattcacct accagaacga caaactgaaa ggccgcgtag
    49081 aagaatttgc cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt gccgaagatg
    49141 ccagcatcga caccatgttc gctgaactgg ggaaagtttg gccgaagttt gacggtttcg
    49201 tacactctat tggttttgca cctggcgatc agctggatgg tgactatgtt aacgccgtta
    49261 ctcgtgaagg cttcaaaatt gcccacgaca tcagctccta cagcttcgtt gcaatggcaa
    49321 aagcttgccg ctccatgctg aatccgggtt ctgccctgct gaccctttcc taccttggcg
    49381 cagagcgcgc tatcccgaac tacaacgtta tgggtctggc aaaagcgtct ctggaagcga
    49441 acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac gccatctctg
    49501 ctggtccgat ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa atgctggctc
    49561 attgcgaagc cgttaccccg attcgccgta ccgttactat tgaagatgtg ggtaactctg
    49621 cggcattcct gtgctccgat ctctctgccg gtatctccgg tgaagtagtc cacgttgacg
    49681 gcggtttcag catcgctgca atgaacgaac tcgaactgaa ataatcgttc tgttggtaaa
    49741 gatgggcggc gttctgccgc ccgttatctc tgttatacct ttctgatatt tgttatcgcc
    49801 gatccgtctt tctccccttc ccgccttgcg tcaggataac gatttccttt acgaccaagg
    49861 agcgcccatg gaacaacgcc acatcaccgg caaaagccac tggtatcatg aaacgcaatc
    49921 cagtactacg gagtatgacg ttctgcctct ggtcccggaa gccgcaaagg tcagcgatcc
    49981 ctttctgctc gacgtgatcc ttgatgaaga gacgctggcc cccttccttt catggctggt
    50041 ccctgcgcgc gttcttgcag tggagttgtt ccctgaccag cttaccgtga cccgttcaca
    50101 aaccttcacc gcttatgaac gcttgtcgac ggccctgacg gttgctcagg tttgcggcgt
    50161 ccagcggtta tgtaactact attcggcgcg acttacgccg ctccccgggc ctgattcctc
    50221 cagggaaagt aatcatcggt tggcacaaat cacgcaatat gcccgccaac tggctagctc
    50281 gccttctatt atcgacaacc gatcgcgcca gcatctgaat gacgtcggtc ttactgcctg
    50341 ggactgtgtg atcattaacc aaatcattgg ttttattggc tttcaggcgc ggaccattgc
    50401 gacatttcag gcttatctcg ggcacccggt acgctggtta cccggtctgg aaatacaaaa
    50461 ctacgccgac gcgtcactgt ttgctgatga atcaatacgc tggcgaagca gctatgaggt
    50521 ggaaaaacta cctgaagagt acacaaaaag ttcaactgca gaactttgcc aactggccga
    50581 aacactctct ctccacccta tttcactttc ccttctcgaa aggttgttaa acagtacacg
    50641 ggttaataca cagccggata ataagcttgc ggcgttgtta tgcgcgcgga taaatggcag
    50701 tcctgcttgt tttgccgcct gtatggattc atcaaatgaa tataaaaaaa tcagcgccct
    50761 tgtgcgcaag ggcgaaaatg aaattaacca atgggctgac cgtcattctg ttgagcacgc
    50821 taccgttcag gcgatacaat ggctgacccg agcacccgat cgctttagcg ccgcccagtt
    50881 cagcccttta ctcgaacacg aaaaatcatc aacgcagatt attaatctgc tggtatggag
    50941 cgggctgtgt ggctggataa atcgcttaaa aatcgcgttg ggtgagacat attaaccttg
    51001 ccgcgtcaga cagattcgcg taaaactgtc agccgctcta atggccacca aaatagacaa
    51061 ttatgtttca ggacaacccg ctgctagcgc agcttaaaca gcaactgcat tcccagacgc
    51121 cacgcgctga aggggtggta aaagccacag aaaaaggctt tggcttcctg gaagtcgacg
    51181 cgcaaaaaag ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat ggcgaccgaa
    51241 ttatcgcggt gatccacagt gaaaaagaac gtgaatccgc agagccagaa gaactggttg
    51301 aaccgttcct gactcgtttc gtgggtaaag ttcagggcaa aaatgaccgt ctggccatcg
    51361 ttcctgatca tccactctta aaagacgcca ttccttgccg cgcagcccgt ggcctgaacc
    51421 acgagtttaa agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg ctgaaaggcg
    51481 atcgttcttt ctatgctgaa ctgacacaat acatcacttt tggtgacgac cactttgtac
    51541 cgtggtgggt tacccttgcg cgccataatc tggaaaaaga agcaccagac ggcgtcgcta
    51601 ccgaaatgct cgatgaaggt ctggttcgtg aagatctgac cgcgctggat tttgtcacca
    51661 tcgacagtgc cagcacagaa gatatggatg acgccctttt cgctaaggcg ttgccggatg
    51721 acaaacttca gctgattgtg gcgattgccg atccaaccgc gtggattgct gaaggcagca
    51781 agctggacaa agccgcgaaa attcgcgcat tcaccaacta tctgcctggc ttcaacatcc
    51841 ctatgctgcc tcgcgagctt tctgacgatc tctgctcact gcgcgccaat gaagtccgcc
    51901 cggtactggc atgccgcatg acgctctccg ctgatggcac cattgaagat aatatcgaat
    51961 tctttgccgc caccatcgaa tccaaagcaa agctggtgta tgaccaggtt tctgactggc
    52021 tggaaaatac cggtgactgg cagcctgaaa gtgaagcaat tgccgaacaa gtccgtttgc
    52081 tagcgcaaat ttgccaacgc cgcggcgagt ggcgtcataa ccacgcactg gtgtttaaag
    52141 atcgcccgga ttaccgcttt attctcggtg aaaaaggtga agtgctggat atcgtcgccg
    52201 agcctcgtcg cattgccaac cgtatcgtcg aagaagcgat gattgccgct aacatttgtg
    52261 cggcccgcgt actgcgcgat aagctcggtt ttggcatcta taacgtgcat atgggctttg
    52321 atccggcgaa tgccgacgcg ctggcagcgt tgctgaaaac gcacggtctg catgtcgatg
    52381 ccgaagaagt gctcacgctg gacggtttct gcaaactgcg tcgtgaactg gatgcgcaac
    52441 caactggttt cctcgacagc cgcattcgtc gcttccagtc atttgctgaa attagcactg
    52501 aacccggtcc tcactttggc ctcggtctgg aagcatacgc cacctggact tcgccgatcc
    52561 gtaaatatgg cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa ggcgaaactg
    52621 cgacgcgccc acaggatgaa atcactgtcc aaatggccga gcgtcgccgt ctcaaccgga
    52681 tggcagaacg tgatgttggc gactggttat acgcacgctt cctgaaagac aaagccggga
    52741 ccgacacccg tttcgcggcg gaaattgtcg atatcagccg tggcggcatg cgtgtgcgtt
    52801 tggttgataa cggcgctatc gcctttattc cggcaccttt cttacacgct atgcgcgatg
    52861 aactggtttg cagccaggaa aacggcactg tacaaattaa aggtgaaacg gtttacaaag
    52921 taactgacgt tattgacgtc accattgccg aagtccgcat ggaaacccgc agcattattg
    52981 cgcgcccggt cgcgtaatct cctttcacgg cccattcctc atggatgggc cgtttatttc
    53041 cccgctctac cttcgttata tccaggcaga ttttttaact gactttcgtt tgaaaactgg
    53101 cgtttttcca aaactggatt acaaataaat atataaaaaa aacaatgcgt ttagccgccg
    53161 ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc tgttaaccgg
    53221 aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt tctcggatcg
    53281 cacaatccat actggcggtt gacggaaagc agcgatgttt tgcgcttttc taccaccgaa
    53341 accacagaac ctgatcgtac attgcagtta tctgccgaac aggctgctcg catcagggaa
    53401 atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgaaag cgatctttct
    53461 gtgcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc gtctgcatgg
    53521 catgacacac cggcggttgc tcgtgattta tcacacgggc tttcctttgc tgagcaggta
    53581 gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat ccaacgcttc
    53641 aatcggttat gtgaagatta cactgggttg aaagaacacg acgtcattgg ccaaagcgtg
    53701 tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa ccgtgtattt
    53761 tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacacgtaa aggccagcgg
    53821 ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga gattttttta
    53881 atctgttccg gcaccgacat taccgaagag cgccgcgctc aggagcgact gcgtattctg
    53941 gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga tttaatcgat
    54001 cacgctatta atcatgcaga taacaataaa gttggggttg tgtatcttga tttggataat
    54061 ttcaaaaagg tcaacgacgc ctatgggcat ttgtttggtg accagttatt acgcgacgtg
    54121 tcattggcta ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc aggtggcgat
    54181 gagtttctgg tactggcatc caacacctca caaagcgcgc tggaagcaat ggcatcacga
    54241 attttgaccc gcttacggct cccctttcgc attggtttaa ttgaagttta taccagctgt
    54301 tcagtaggta ttgcactctc tcccgaacat ggttcagaca gcacggctat tattcgtcac
    54361 gccgacacag caatgtacac agcgaaggaa ggcggacgag gacaattttg cgtttttacc
    54421 ccagaaatga atcaacgggt atttgaatat ctctggctgg ataccaactt gcgtaaagca
    54481 ctggaaaacg atcagttggt tattcactat caaccgaaaa tcacctggcg tggcgaagtg
    54541 cgcagtctgg aagcactagt acgttggcag tcacctgaac gtgggttgat tccaccgttg
    54601 gacttcattt cctacgccga agagtcaggg ctaattgtgc ctttaggccg ttgggtgatt
    54661 ctcgatgtcg tacgccaggt ggcgaagtgg cgggataaag gtattaacct gcgagtggcg
    54721 gtaaatattt ctgcacgtca gctcgccgat caaaccattt tcaccgccct gaaacaggtt
    54781 ctccaggaac tcaattttga atactgccct atagatgttg aactgacaga gagttgtctg
    54841 attgagaatg atgaactggc actgtctgtt attcaacaat ttagccaact aggtgcgcaa
    54901 gtgcatctgg atgattttgg taccggctac tcttcacttt cgcaactggc gcgctttccg
    54961 atcgatgcca tcaaacttga ccaggttttt gttcgagata ttcacaaaca acctgtctcg
    55021 cagtcactgg tccgggcgat cgtcgctgtg gcccaggcat tgaatcttca ggtgatcgcc
    55081 gaaggtgtag agagtgcaaa ggaagatgct tttttaacca agaacgggat caatgagcgg
    55141 caaggatttt tgtttgccaa accgatgccc gccgtcgcct tcgaacgctg gtataaacgc
    55201 tatctgaagc gcgcataagt agatgaatat atatacttag cgctatatcc cgtcattata
    55261 tatcgtattc ctggatgata attagccgtt gctgttaaac acttctcttt tagcggcaat
    55321 ttttgcatta ttcattttcc acttttgcta taaggatgtc cttgatgtct gaatttgatg
    55381 cccagcgcgt tgccgaacgt attgatattg tgttagatat ccttgtggca ggcgattacc
    55441 actctgcgat ccataatctc gagatactta aagcagaact cttgcgccag gttgctgaat
    55501 ctacgccaga catcccgaag gcaccgtggg agatttaaca atcgtgcctt caggttttcc
    55561 tatttgagga tgcccggcgc gtcagtattt ttttatttag tattataacg ttataagaat
    55621 tacagcgatg aattcccgac aacaaactat tctacagatg gtcattgacc agggtcaggt
    55681 tagcgtaacc gatctggcaa aagccactgg agtttctgaa gttaccattc gccaggatct
    55741 caacaccctc gaaaaactga gttacctccg ccgtgcacat ggctttgcag tttcgcttga
    55801 tagtgatgac gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg aactcgccga
    55861 gtttgccgcg tcactggttc aaccgggcga aaccatcttt atcgaaaatg gtagcagcaa
    55921 tgccctgctc gcccggactc tgggcgaaca gaagaaaaat gtcactatca tcacagtcag
    55981 cagctacatt gcgcatttgc tgaaagacgc gccttgtgaa gtcattttgc tcggtggcgt
    56041 gtaccagaaa aaaagcgaaa gtatggttgg ccctttgaca cgccagtgca tccagcaggt
    56101 atatttcagc aaagcattta ttggtattga tggctggcaa cctgaaactg gattcaccgg
    56161 tcgcgatatg atgcgtaccg atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat
    56221 agtcctgact gacagctcga aatttggtgc tgtacattca tactccatcg gtcccgttga
    56281 gcgattcaat cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga tgcatcttga
    56341 gcacagtaaa ctcaccgttc acgtcgttga catttaacca tttcccttct ctacggatga
    56401 tttgcagttt ggcaaatcat ccgctctaag atgattcctg gttgataatt aagactattt
    56461 acctgttatt aacactctca agatataaaa ttattatcag cgatataaca ggaagtcatt
    56521 atcacctgcg tgatataacc ctgcgcgcga gcagatttca cggaataatt tcaccaggct
    56581 tattcttagc tattatagtt atagagagct tacttccgtg aatcataaat tcaggagaga
    56641 gtattatgtt tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt accctggcaa
    56701 tgtctctgag tgcctgttct aactggtcta aacgggaccg caacaccgca atcggcgcgg
    56761 gtgcaggggc attaggcggt gcagtactga ccgatggcag tacgttgggt acattaggtg
    56821 gtgcagctgt cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg
    56881 attatcgact cgcaattata atacgcttga ttctgttcag tacgaaataa ctgaataata
    56941 cacacagtaa aatattc
//
LOCUS       DAZFFI010000020        54972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFFI010000020 DAZFFI010000000
VERSION     DAZFFI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.020"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..176
                     /locus_tag="ACHJ12_001456"
     CDS             <1..176
                     /locus_tag="ACHJ12_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO4989274.1"
                     /translation="HFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFF
                     TNFNTIERIENYL"
     gene            173..652
                     /locus_tag="ACHJ12_001457"
     CDS             173..652
                     /locus_tag="ACHJ12_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO4989275.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(706..1656)
                     /gene="corA"
                     /locus_tag="ACHJ12_001458"
     CDS             complement(706..1656)
                     /gene="corA"
                     /locus_tag="ACHJ12_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4989276.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1808..1864)
                     /gene="ysgD"
                     /locus_tag="ACHJ12_001459"
     CDS             complement(1808..1864)
                     /gene="ysgD"
                     /locus_tag="ACHJ12_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4989277.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2026..2794
                     /gene="yigE"
                     /locus_tag="ACHJ12_001460"
                     /pseudo
     CDS             2026..2794
                     /gene="yigE"
                     /locus_tag="ACHJ12_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
     gene            complement(2941..5103)
                     /gene="uvrD"
                     /locus_tag="ACHJ12_001461"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2941..5103)
                     /gene="uvrD"
                     /locus_tag="ACHJ12_001461"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4989278.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5187..5903)
                     /gene="yigB"
                     /locus_tag="ACHJ12_001462"
     CDS             complement(5187..5903)
                     /gene="yigB"
                     /locus_tag="ACHJ12_001462"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4989279.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5903..6799)
                     /gene="xerC"
                     /locus_tag="ACHJ12_001463"
     CDS             complement(5903..6799)
                     /gene="xerC"
                     /locus_tag="ACHJ12_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4989280.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6796..7503)
                     /gene="yigA"
                     /locus_tag="ACHJ12_001464"
     CDS             complement(6796..7503)
                     /gene="yigA"
                     /locus_tag="ACHJ12_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO4989281.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7500..8324)
                     /gene="dapF"
                     /locus_tag="ACHJ12_001465"
     CDS             complement(7500..8324)
                     /gene="dapF"
                     /locus_tag="ACHJ12_001465"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4989282.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8361..8564)
                     /gene="yifL"
                     /locus_tag="ACHJ12_001466"
     CDS             complement(8361..8564)
                     /gene="yifL"
                     /locus_tag="ACHJ12_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4989283.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8914..9468
                     /locus_tag="ACHJ12_001467"
     CDS             8914..9468
                     /locus_tag="ACHJ12_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO4989284.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            9806..11314
                     /locus_tag="ACHJ12_001468"
     CDS             9806..11314
                     /locus_tag="ACHJ12_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4989285.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FHPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11311..12624
                     /locus_tag="ACHJ12_001469"
     CDS             11311..12624
                     /locus_tag="ACHJ12_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO4989286.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            12672..12992
                     /gene="cyaY"
                     /locus_tag="ACHJ12_001470"
     CDS             12672..12992
                     /gene="cyaY"
                     /locus_tag="ACHJ12_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4989287.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13032..15578)
                     /gene="cyaA"
                     /locus_tag="ACHJ12_001471"
     CDS             complement(13032..15578)
                     /gene="cyaA"
                     /locus_tag="ACHJ12_001471"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4989288.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            15965..16906
                     /gene="hemC"
                     /locus_tag="ACHJ12_001472"
     CDS             15965..16906
                     /gene="hemC"
                     /locus_tag="ACHJ12_001472"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4989289.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            16903..17643
                     /gene="hemD"
                     /locus_tag="ACHJ12_001473"
     CDS             16903..17643
                     /gene="hemD"
                     /locus_tag="ACHJ12_001473"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4989290.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17665..18876
                     /gene="hemX"
                     /locus_tag="ACHJ12_001474"
     CDS             17665..18876
                     /gene="hemX"
                     /locus_tag="ACHJ12_001474"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4989291.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            18879..20075
                     /gene="hemY"
                     /locus_tag="ACHJ12_001475"
     CDS             18879..20075
                     /gene="hemY"
                     /locus_tag="ACHJ12_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4989292.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKLEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            20754..20891
                     /locus_tag="ACHJ12_001476"
                     /pseudo
     CDS             20754..20891
                     /locus_tag="ACHJ12_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(20999..22234)
                     /gene="aslB"
                     /locus_tag="ACHJ12_001477"
     CDS             complement(20999..22234)
                     /gene="aslB"
                     /locus_tag="ACHJ12_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4989293.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELKEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(22381..22457)
                     /locus_tag="ACHJ12_001478"
     tRNA            complement(22381..22457)
                     /locus_tag="ACHJ12_001478"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22421..22423),aa:Pro,seq:tgg)
     gene            complement(22500..22586)
                     /locus_tag="ACHJ12_001479"
     tRNA            complement(22500..22586)
                     /locus_tag="ACHJ12_001479"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22550..22552),aa:Leu,seq:cag)
     gene            complement(22607..22682)
                     /locus_tag="ACHJ12_001480"
     tRNA            complement(22607..22682)
                     /locus_tag="ACHJ12_001480"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22647..22649),aa:His,seq:gtg)
     gene            complement(22741..22817)
                     /locus_tag="ACHJ12_001481"
     tRNA            complement(22741..22817)
                     /locus_tag="ACHJ12_001481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22781..22783),aa:Arg,seq:ccg)
     gene            complement(22920..24305)
                     /gene="yifK"
                     /locus_tag="ACHJ12_001482"
     CDS             complement(22920..24305)
                     /gene="yifK"
                     /locus_tag="ACHJ12_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4989294.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24496..25236)
                     /gene="wecG"
                     /locus_tag="ACHJ12_001483"
     CDS             complement(24496..25236)
                     /gene="wecG"
                     /locus_tag="ACHJ12_001483"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4989295.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGHLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAKTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25239..26591)
                     /gene="wzyE"
                     /locus_tag="ACHJ12_001484"
     CDS             complement(25239..26591)
                     /gene="wzyE"
                     /locus_tag="ACHJ12_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4989296.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26588..27667)
                     /gene="rffT"
                     /locus_tag="ACHJ12_001485"
                     /gene_synonym="wecF"
     CDS             complement(26588..27667)
                     /gene="rffT"
                     /locus_tag="ACHJ12_001485"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4989297.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(27664..28914)
                     /gene="wzxE"
                     /locus_tag="ACHJ12_001486"
     CDS             complement(27664..28914)
                     /gene="wzxE"
                     /locus_tag="ACHJ12_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4989298.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKATLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(28916..30046)
                     /gene="rffA"
                     /locus_tag="ACHJ12_001487"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28916..30046)
                     /gene="rffA"
                     /locus_tag="ACHJ12_001487"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4989299.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30051..30725)
                     /gene="rffC"
                     /locus_tag="ACHJ12_001488"
                     /gene_synonym="wecD"
     CDS             complement(30051..30725)
                     /gene="rffC"
                     /locus_tag="ACHJ12_001488"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4989300.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30703..31584)
                     /gene="rfbA"
                     /locus_tag="ACHJ12_001489"
     CDS             complement(30703..31584)
                     /gene="rfbA"
                     /locus_tag="ACHJ12_001489"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4989301.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31603..32670)
                     /gene="rffG"
                     /locus_tag="ACHJ12_001490"
     CDS             complement(31603..32670)
                     /gene="rffG"
                     /locus_tag="ACHJ12_001490"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4989302.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32670..33932)
                     /gene="wecC"
                     /locus_tag="ACHJ12_001491"
     CDS             complement(32670..33932)
                     /gene="wecC"
                     /locus_tag="ACHJ12_001491"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4989303.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(33929..35059)
                     /gene="wecB"
                     /locus_tag="ACHJ12_001492"
     CDS             complement(33929..35059)
                     /gene="wecB"
                     /locus_tag="ACHJ12_001492"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4989304.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35115..36161)
                     /gene="wzzE"
                     /locus_tag="ACHJ12_001493"
     CDS             complement(35115..36161)
                     /gene="wzzE"
                     /locus_tag="ACHJ12_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4989305.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36173..37276)
                     /gene="wecA"
                     /locus_tag="ACHJ12_001494"
     CDS             complement(36173..37276)
                     /gene="wecA"
                     /locus_tag="ACHJ12_001494"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4989306.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37516..38775)
                     /gene="rho"
                     /locus_tag="ACHJ12_001495"
     CDS             complement(37516..38775)
                     /gene="rho"
                     /locus_tag="ACHJ12_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4989307.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(38860..38961)
                     /gene="rhoL"
                     /locus_tag="ACHJ12_001496"
     CDS             complement(38860..38961)
                     /gene="rhoL"
                     /locus_tag="ACHJ12_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4989308.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39102..39431)
                     /gene="trxA"
                     /locus_tag="ACHJ12_001497"
     CDS             complement(39102..39431)
                     /gene="trxA"
                     /locus_tag="ACHJ12_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4989309.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39562..40827
                     /gene="rhlB"
                     /locus_tag="ACHJ12_001498"
     CDS             39562..40827
                     /gene="rhlB"
                     /locus_tag="ACHJ12_001498"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4989310.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            40961..42445
                     /gene="gppA"
                     /locus_tag="ACHJ12_001499"
     CDS             40961..42445
                     /gene="gppA"
                     /locus_tag="ACHJ12_001499"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4989311.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42492..44513)
                     /gene="rep"
                     /locus_tag="ACHJ12_001500"
     CDS             complement(42492..44513)
                     /gene="rep"
                     /locus_tag="ACHJ12_001500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4989312.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44730..45179
                     /locus_tag="ACHJ12_001501"
     CDS             44730..45179
                     /locus_tag="ACHJ12_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4989313.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVNNGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45378..45659
                     /gene="ppiC"
                     /locus_tag="ACHJ12_001502"
     CDS             45378..45659
                     /gene="ppiC"
                     /locus_tag="ACHJ12_001502"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4989314.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45746..47221)
                     /gene="ilvC"
                     /locus_tag="ACHJ12_001503"
     CDS             complement(45746..47221)
                     /gene="ilvC"
                     /locus_tag="ACHJ12_001503"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4989315.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQDKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47371..48264
                     /gene="ilvY"
                     /locus_tag="ACHJ12_001504"
     CDS             47371..48264
                     /gene="ilvY"
                     /locus_tag="ACHJ12_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4989316.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48316..49860)
                     /gene="ilvA"
                     /locus_tag="ACHJ12_001505"
     CDS             complement(48316..49860)
                     /gene="ilvA"
                     /locus_tag="ACHJ12_001505"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4989317.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(49863..51713)
                     /gene="ilvD"
                     /locus_tag="ACHJ12_001506"
     CDS             complement(49863..51713)
                     /gene="ilvD"
                     /locus_tag="ACHJ12_001506"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4989318.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMESGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGNIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(51778..52707)
                     /gene="ilvE"
                     /locus_tag="ACHJ12_001507"
     CDS             complement(51778..52707)
                     /gene="ilvE"
                     /locus_tag="ACHJ12_001507"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4989319.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52727..52990)
                     /gene="ilvM"
                     /locus_tag="ACHJ12_001508"
     CDS             complement(52727..52990)
                     /gene="ilvM"
                     /locus_tag="ACHJ12_001508"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4989320.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(52987..54633)
                     /gene="ilvG"
                     /locus_tag="ACHJ12_001509"
     CDS             complement(52987..54633)
                     /gene="ilvG"
                     /locus_tag="ACHJ12_001509"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4989321.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54636..54686)
                     /gene="ilvX"
                     /locus_tag="ACHJ12_001510"
     CDS             complement(54636..54686)
                     /gene="ilvX"
                     /locus_tag="ACHJ12_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4989322.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(54773..54871)
                     /gene="ilvL"
                     /locus_tag="ACHJ12_001511"
     CDS             complement(54773..54871)
                     /gene="ilvL"
                     /locus_tag="ACHJ12_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4989323.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT        13469 a        14780 c        13951 g        12772 t
ORIGIN      
        1 ttcattttgt agcacaggac cacttcggtc ttgatgtaac tgatatcaaa aacaagactt
       61 acagcaaata tagatttttt cgcatatggt tctttttaca acggcataaa gactttgctt
      121 tcaaaccttt tttcaccaat tttaacacta tcgaaagaat cgaaaattat ctatgaaaag
      181 caaactccag aaaattatat tatgtttatt tcttctatgc tgcatctata atttatggac
      241 attgcgtcct gtacagattt tgtatacata ctctgatgct ggcaattcag tctttctggt
      301 cgttgatcat ctgccatgga ctgacagcga taaaataaat tggtatctga agcatcagaa
      361 tgaaattaaa aatcaacatc ccctaccaga aggtagttgg catacctggt atgtcattga
      421 tatcggtaat ggcttcactg actataaaaa atatatcgaa gggccgtatg aagatctcta
      481 ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg aaaaattact taatggttat
      541 taatgaatac ccttatcgta acactcatat tggtattaac gattttactg agtatcaatt
      601 aacacaagaa aataaaatag agcgagtttt caatccgcat gactttaaat aagacaattt
      661 ttagaaataa gccggataaa aaaatagctt tatccggcac tcatattaca gccagttctt
      721 gcgcttaaag tacagatacg gtgccaggcc cgcgaggatc ataaagataa tcgcgcctgg
      781 gtagccgaag ctccacttca gttctggcat aaactcaaag ttcatcccat agctggaagc
      841 aacgagcgtc ggcggcagga ataccacgga taccaccgag aagattttga tgatgcggtt
      901 ctgctcgatg ttgataaagc ccatcgccgc ctgcatcagg aagttcacct tctggaacag
      961 ggattcgtta tgcggcagca gggattcgat atctcgcagg atttcacgcg cctgctccag
     1021 ttgcccaccc ggtaaacgcg ccttgcgtac caggaagttg agcgcacgct gggtatccat
     1081 cagacacagg cgaactttcc agccgatatc ttccagttcc gccagcgtgg agagcgcctc
     1141 atcgtactca tcgccctgat gcccttccat aattacccgg ctcaactgct ccagatcgct
     1201 gtagatattt tcaatttcat ctgccagctg ttcgattttg gtttcgaaca gatccaacag
     1261 cagctcgtag gcgttaccgt ccaccattga ctggctacgg gcgcgcatac gatacagacg
     1321 gaatgcaggc agttcacgct cacgcagagt aaacagacga ccatcacgga tggtaaatgc
     1381 cacagtggag ttaccggcgt gatcttccgc atcttcaaag aagaagaagg agtgaatatg
     1441 caggccgtca tcgtcttcaa agaaacgtgc cgatgcttcg atgtcttcca gttccgggcg
     1501 ggttgccagg ctctggccaa gttcagattg tacgcgcagt cgctcgtcgt cgtccggttc
     1561 gacaagatca atccatactg catttacaag gggttgtgac tcttcgactt ccagccgggt
     1621 cagtcggtta ttttccagtt gaaatgcgct cagcatgacc gggactccca atgcttaaaa
     1681 tatcggacag ttcggtgggc acacagaaac aaattgggtt tcagaccatt aaacagcctg
     1741 actcagcgcg acgggaaaaa ttgaggtcgc tgacaaccgc taaggctatc agcaaaaagg
     1801 gatagcctta ggagttgatc ctggatgaca ggatagtgag ccagtatcta ctgggtgtgt
     1861 ccaaggcgaa tgtcctctta gagtgatcgt gcgcgcatgt tacgccagca aaattttgcc
     1921 gtcaacacgc aacagaacac cacagaacaa tagttgaact tcgcgctttt tggtactcgt
     1981 caaggtcgct aacaggataa aaaaatccaa catccaggac ctataatggc gcatcggcta
     2041 ctcattggta aaggaatgat caccttgaat ctcaaacgga tttttctcgc cctcacctta
     2101 cttcccttgt ttgctgttgc cgctgatgat tgcgcactct cagatccgac gctgaccgta
     2161 caggcgtata ccgttaatcc tcaaacagag cgggtgaaaa tgtactggca aaaagccaat
     2221 ggcgaagcgt ggggaacttt acatgctctg ctggcggata tgaatagtca gggtcaggtg
     2281 cagatggcga tgaacggcgg catctatgat gaaagctatg cgccgctcgg tttgtacatc
     2341 gaaaacggtc agcagaaggt ggcgttaaat ctcgcttcag gtgaagggaa tttctttatc
     2401 cgtcctggcg gcgtgtttta tgtcgcggga gataaagtcg gcatcgttcg tctggatgcc
     2461 ttcaaaacca gtaaagagat tcagtttgcg gtgcagtcag ggccaatgtt gatggaaaac
     2521 agtgtaatta atccgcgtat tcatcccaac gtcgcctcac gtaaaattcg taacggtgtt
     2581 gggattaata aacatgggaa tgccgtgttt ttgttgagcc agcaggcgac aaatttttat
     2641 gattttgcct gttatgccag ccaaagcgaa actgaacgtt gagcagctgc tttatcttga
     2701 tggcacgatt tcacatatgt atatgaaagg cggggcaatc ccgtggcaac gctatccttt
     2761 tgtcaccatg atttccgtag agcggaaagg ttaaaacgat agttttgtgt cagatactga
     2821 agatggcgca gatgccggat gcggcgtaaa cgccctatcc ggcctacatg acgttgcaat
     2881 ttattgaata tgaatgattt tttaggccaa ataaggtgcg cagcaccgca tccggcaacg
     2941 ttacaccgac tccagccggg cgtatgccgc caccagccat ttaataccct ggccctggaa
     3001 tgccacctgc aaacggctat gctcgccgct gccttccata ttgacgatgg tgccttcacc
     3061 aaacttagcg tggcgtacac gctgaccgag cttgtagccg ctgtcgttct cgaccatcgg
     3121 cgtccccatc cgttgatggc tgaccggacg acttaccgta gcgcgcaggc gcacctcttc
     3181 cacacactct tccggcagct cgccgataaa gcgcgacggg cgatggtaaa cctctttacc
     3241 atacagacgg cgggtttccg cgtaggtcag cgtcagtttc tgcatcgcgc gggttacacc
     3301 aacgtaggcc agacggcgtt cttcttccag acgcccgcct tcatccagcg acatctggct
     3361 tgggaacata ccctcttcca taccgacaat aaacacctgc gggaactcca ggcctttcgc
     3421 cgagtgcagc gtcatcaact gcactgcatc ctgccaggta tccgcctgcc cctcgcccgc
     3481 ttccagcgcc gcatgggaga ggaatgcctg cagcggcatt aaatcttcat cttcttcgtt
     3541 gtagctgaac tggcgcgttg ccgtcaccag ttcctctaag ttttcgatac gcgtctggcc
     3601 tttttcgccc ttctcctgct cgtacatggt gcgcaggccg gagtctttaa ttacccggtc
     3661 agtctgtaca tgcagcggca tatcggcagt ttcttgcgct aaggcatcga ttaattccat
     3721 aaatcgctgc aaggcgctgg cagctcgccc ggcgagggct ttttcctgca acagttcacg
     3781 acatgcctgc cagagtgtta actggcgatc gcgcgatgtc tgacgtacca cgtccagcgt
     3841 ccggtcacca ataccccgcg ttggcgtatt caccacgcgc tcaaacgccg cgtcgtcgtt
     3901 acggttggca atcagacgca gatacgagag cgcgtctttg atttcctggc gttcgaagaa
     3961 gcgcatcccg ccgtaaatac ggtacggcat actggcctgc aacagtgctt cttccagtac
     4021 acgcgactgg gcgttactgc ggtagagaat ggcgcactcg gcaagcgccc cgccgttgtc
     4081 ctgccaggtt ttgatgcggt taaccacaaa acgcgcttca tcgagttcgt taaaagcgca
     4141 atagagggaa ataggctcac cgtccgcgcc atcggtccac agttttttac ccagacgccc
     4201 gttattgttt tcaatcaggg cgttagcggc gctcagaata ttgctggtag agcggtagtt
     4261 ttgctccaga cgaatagttt cggcaccggg gaaatcatta aggaaacgct gaatattctc
     4321 cacctgcgcc ccgcgccagc cgtagattga ctggtcgtca tcaccgacga tcatcacttt
     4381 gccggtgtcg cccgccagca ggcggatcca cgcgtactga atgttgttgg tatcctggaa
     4441 ttcgtccacc aggatattgg taaaacgttc gcggtagtgt tgtaggatat gcggcttgtt
     4501 aagccacagt tcgtgagcgc gcagcagcag ctcggcgaag tccaccaggc ccgcgcggtc
     4561 acacgcttcc tgatacgcct gatacacctt ctgccaggtc tgctccaccg gattaccgta
     4621 gctttgaata tgatgcggac gcaggccttc atctttctgg ctgttgatgt accacattgc
     4681 ctggcgcggc ggccactgct tctcgtcgag gttcatggct ttgatcagac gcttaagcag
     4741 gcgcagctgg tcctcgctat cgaggatctg gaaatcctgc ggcagattgg cgtccatatg
     4801 gtgcgcacgc agcaggcggt gcgccagccc gtggaaggtg ccgacccaca tgccgccctg
     4861 gctggtgccc atcagttgcc cgatacgatg acgcatctcc gccgccgctt tgttggtaaa
     4921 cgtcaccgcc ataatcgagt atggcgagca gttttccacg ctcatcagcc aggcgatacg
     4981 atgcaccagt acgcgcgtct taccactgcc cgcgcccgcc agcaccagaa ggttgctgcg
     5041 tggcgcggcc accgcttcgc gctgtttgtc attaaggctg tcgagcaggt aagaaacgtc
     5101 cattggcacc gccgcgtaaa aataggttgg gcggagaagc gcaacgccct ccgccaaaaa
     5161 gctgggtata tatacagatt tgctgattat atcagcgagg tcagagatgc caaccgggaa
     5221 atttccagat gcggcagtaa acggctgtcc caggtttgca tcagatcgcc attttccggt
     5281 ctgatccaac aagcctgcat tccgctgcga attgccccac ccacgtcagt ggtgagatcg
     5341 tccccaacat gtaagatctc gccgatcggc acgttgagtt tttccgcagc caaaaagtac
     5401 atatcgctga acggttttga gcgcccgtgc gggccagcgc gcagcacaaa ctcaaaataa
     5461 tcccccaaac caaacagttc cggctgggcg ttaccgttgg tgatcgccac cagcggccat
     5521 ttcttcgcca gctgttttaa ggtgtcgtga gtttgctgcg ggacgtcgat tcggctgcgc
     5581 catttggcaa agttaatcat tgctgcgtgt gcgcctgcac tggcttcttc ggcactcagc
     5641 ccggcgtcga gcatcgcttg ttcaatcgaa cgaaaacgcc aacgcgtcac gtcgtgataa
     5701 atctcgggtt ccgcttcccg taccgcctgg cgcaggcgtt gcagatcttc attctggaag
     5761 ctgcgcagcg ccggatgata attttgcaca aaggtaagcg cctctcgctc ggtgcgcaaa
     5821 atcaccggac ggttatcgta aagggtatca tccaggtcaa aggtgagcgc cgagatgcgc
     5881 cccaaaggcc ggtaaaaacg cattatttcc cccgtttggc gcgtggatgc gccgcatcgt
     5941 acaccgaggc aaggtgttga aaatcaagat gagtatagat ttgcgtggtg gagaggttgg
     6001 catgacccag cagctcctgc acaccacgaa gatcgccgct cgactccagc atatgcgtag
     6061 cgaacgagtg acgtaattta tgtgggtgaa cgtgattatt cagcccttgt tttatgcccc
     6121 attcggcaaa gcgtttctgc acattacgcg cggagatacg cttgcccagt ttcgacagaa
     6181 aaagcgcgtc gtcttcgcta ccaaacaggt cgcgcaaatc aagccagtgc tcaatccacg
     6241 ccacagcgtt gcgaccaatc ggcaggcggc gctctttgct gcctttcccc attacccaca
     6301 cttcaccaga ctccaggtcg aggtgtttga tatccagccc caccagctcg gaaagacgca
     6361 gacccgcgcc gtacatcact tccagcattg cacggtcgcg tacagcgagg ggatcgttga
     6421 tatcaatatc cagcagccga ttcatatcgt cgacgtcgat gtttttcggc agatgacgcg
     6481 gcgctttcgg tgccgaaact cctttcgccg ggttagcttt gagttcgttc tggctgacca
     6541 gccagtcaaa aaagctacgt agcgcagaaa gccgtaacgc gaggcttgct gctcccagcc
     6601 ctttacggcg actgcgtaca gcaaaattgc gcaccatcgt cacatcacat tgctgccagc
     6661 tttgcaggcc gttttcgctg gcaaaattga tgatcgcctc aagctgacgc tggtagttaa
     6721 gtagggttat cgggctaagc tggcgctcca cgctcagata acgtaggtag cgttctacat
     6781 cggtgtgtaa atcggtcata cgcgttcaat ccaacgctcc agaagctccg gcaacatcag
     6841 cgcaatttca tgaagtaact gcgttccttg cccttgttga tagtgactgg catcgcgact
     6901 ggtaaacagc acgacaccca aatcagcatc gcttcccagc atcgacatcg ccaccgatcc
     6961 caccgctttc gcttccggta gcaccaccag cagctctggt ccgttaagcg gcccaagata
     7021 gtgctgttcc tgccccaaac gctgaatacg cagcggttcg aaagactgac ggcttaatgc
     7081 cagatgagtg tggttcgacg gcgcacctaa gcgccagcga tccggaaaca ggcgcagact
     7141 cgcacctgcc aggccgagat cgcgcgccca gcggtgaaag cgcatcagca tatcgtcgag
     7201 actgctggcg gcggtgagac tgcgctgcag gtagagtagg cgataaaaca ggccttcgtt
     7261 ggcgatagcc tgttccatca acagcgccat gttctcttcc agaacatgaa tatgattacg
     7321 tgcgcgggcc atgtgccact cgaccaacga aacggtgccg cgtaccggat gcggcacacg
     7381 tatcgcttct actgcgcgcg cattacggat aaaaaactca ggatttttaa tcagataatc
     7441 gacaaccgcc cggtcatcaa gctccgtgag tgtttcctgc agttcttccc ctggttgctt
     7501 catagatgaa taaatccgtc gtagacatgt accgccgggc cagtcatata taacgggtga
     7561 cccggacctt tccaggcgat atcaagacga ccgccgggga gttccacgcg tacttcttcg
     7621 gccagcaaac cttgctgaat ccctactgca accgccgcac acgcgccgct gccgcaggcc
     7681 tgggtttctc ctgccccacg ctcataaacg cgtaaacgaa tatgctcgcg cttaaccact
     7741 tgcataaaac cgatattggc gcgctccgga aaacgctcgt ggctttccag aacaggacca
     7801 agcgtttcta ccgccgcggt atcgacatca tcgacctgaa tcacgcaatg cggatttccc
     7861 atcgacacca cgccgcataa gattgtctgc tcggcggcgc gcataatata ggtcttttcc
     7921 gctttgttag cgcgaaacgg cacggcggaa ggttcgaagt tgggttcgcc catatttacg
     7981 cggaccagat catcatcggt gacggtcaga accatccgcc cgttggcggt gctgacgcgg
     8041 atatcacgct tattggtcag tcctttcaga cgcacaaaac gggcaaagca gcgcgcaccg
     8101 ttgccgcact gcgccacttc actgccatca gcattgaaaa tgcgatagtg aaaatccagt
     8161 tcaggatcat acggcggctc aaccaccagc agttggtcaa accctacccc caggtgccga
     8221 tcagccaggc gacgaatcag ctccggtgaa aaaaagacat tctgcgttac cgcgtcgacg
     8281 accataaaat cgttgccaag gccatgcatt ttcgagaact gcatcattta ctccaatcac
     8341 gcgggtacag aaactgactt ttagtaattc acctgggatg gaccatcgcc agtggcgcga
     8401 tcgtttttat ccggcaccgt ggattgcgtt tgcgtctcta ccggtttggt cggcggcggt
     8461 gcgtttttat ctgcaggcgg gaaatagagc ggacctttca gaccgcagcc cgtcaggctg
     8521 aagagagtaa gtaatacagt gagtgcctta aacacgtttt tcattatgga ttgcctgtaa
     8581 agttcatcgc tttctgcttt ctatcatcgc aggagaagac gtaaaagcaa gcgtttagcg
     8641 actctttatg aagtctgaaa agtgaggaaa tgctgcgagt ataacgctta ctcgtgggga
     8701 tttatgtttt gctggtagtt tcgtgatcgg cacctcgcgc caggatggct ctatgcacta
     8761 tcgcaatgta gtttgtttgt tggcttgttc gttgttctta tcgtcggcat ggagctttta
     8821 aaagagtggc gcaccatgca attttgttgt ctgaccttag tcgccagtga catacaactt
     8881 tgccaacctg gtgataagag ggaataaaat ctgatggggc tatttaccca actggaaaaa
     8941 tttgaccaaa agcttacgcg cggttatgcc cggtgggggc gctgggtatg gcggacgtta
     9001 attgtcgttc cggtactggt cgtgttgtgg aatataggac aggcagtatg gggaggacca
     9061 agaggtggag tgattctgga aatccacagt gaaattgacc gcccgatact gggattcagt
     9121 gttaacggcg tggcgggcgc gaatgctttt gcaaatggtg gcggttcaac aacctgttgt
     9181 ggtgatgtta gtggggatac ggcagaggtt atctggacat taagtactac tcgtacacag
     9241 tataatgctg gaatgcgtct ggagaaacgg aatatgacgt tacctctccc caaaagagaa
     9301 tggggagaag attttctgca tgtgcatttt atgcctggcg ataaagtgct gctggggtgg
     9361 agtaaagatt ctttttctcc gtatgaagat cttcacaacg gggggtacaa gacccgcgtt
     9421 cggcaggatg tgaaagataa actttatggt acagggaaaa tgaattgata gatttctata
     9481 aaaatcacgt tcggcagaaa gcgtaataat gtggggaatg ttagataccg gtggtgggac
     9541 ctaagtacaa cacgcgaaag acaataaagt aggtaacggt gggcagagtc gcatgttgac
     9601 acccttcccg gcgtcaatcc ggcacatttt aacgtgatca gtcacaccat ggcgcagtag
     9661 ctcgtattta tatcgtgaat gaaaacctca agtttacccc agcaactatc agaaaatagc
     9721 gatggccttt atagattctg aaaagtgacg atttgccgca aaagttacgc ttacgtgcga
     9781 ggaattatgt tctgctggta gtttcatgat caacacctcg cgccaggatg gctttatgca
     9841 ctatcgcaat gtggtttgtt tgttggcttg ttcattgttt ttatcgtcgg catggggatg
     9901 tcggctttcc gcacctgaac ataatattta ccagaaacag ggaaaaggcg tggtgtacct
     9961 gcgcccttat aaagaaacaa atctttctct acctgacgtg aattacaaga gtttgcgtcg
    10021 gttacccaat ttattaatag acccgacaac actagacgaa tgggataaag aaccgcctct
    10081 gactgatttg acaaccgact acctctatga gggcgcacag gcctggtatc ctcattattc
    10141 ctggcatagc gatggtcgct atatcctcta cgcgggtgaa gttgtgcaaa acccgccaga
    10201 caaacctccc gtcgatgtcg cctcgttcaa ggcgtggggt gattttgccg cagataaaca
    10261 cagtctctat ttcgaaggca agcgcacaga taacaatggc gggggaaatc gcttagatat
    10321 caaaacactg catcaggtgg aattccggcc tccctgggat cctgacttac tggggctgat
    10381 attacgtgac gctaatttct tgtatatcaa tgggcatcgc ctcgcagatc ccgatagttt
    10441 ccgtgtgctg gcacaaaagt catgggatca gcgaggaaag ttttctaccg cgtttaatcc
    10501 ctgtgtggct atgccatttg gcccctggga taccctggct cgtacacgga ccaaaatcct
    10561 gatcaacggc gaacagcttg atgccgaccc ggacaccttt tccgtcgtgc gctggatgcc
    10621 cggctcactc ttgacctggc gtgataaaaa cgggctgcag cgtaaagtcc tcgacaagga
    10681 aaatctggcg tgggatgaag atttaacaaa gcactgtctg gatttttctc tgctggaaaa
    10741 gaaagtgttc tggcgtaaag gacctgcttg taaacaggaa gaattacccg gactcgatcc
    10801 ggagcagttt caccccatca gtgatgctgt cgcccagtat caggactcgc tttataccat
    10861 catcgaaaca gagtctggtg accgcaagct ggaaatcgtg aaacttgatg atcccaatct
    10921 tattatcaac aaacgtttca acgccgggaa acgccacggc tatttactta cgcgtgccga
    10981 agggtggcca taccattccg gtttacatgt gtttgaatct gacggaccgc tgatcttact
    11041 ggataaccgt tctccggatg aacgcgaagc ccatcttaat gaccatccct ttttgcgcag
    11101 atggtatgcc cgcgataacc gctacattta cagctttgat ggcgcgcagc tctggcgata
    11161 ccgcaccgct gatccgaaac aagttcgctt aatctggaag gaacaacatt cgggatatgg
    11221 ctatggcgta aattacaaaa cgggatatct ggacggaaaa attactgatg acggcgaatt
    11281 tattcctgcc ccgcgcaatg aggcgacaaa atgaagggtc aaataatcac agttgcactc
    11341 atcctgttgg gacacacatt tctgacgcct gctgtacagg ccatagggtt cgactattac
    11401 aatgatcacg gtgttatgtc ttacggtaaa ggttattggg gggataagaa aatcattgcc
    11461 cagtttccgg agatgagcag ggccgatttg cgccttgtga aaaatatctc tgggaaaacg
    11521 ggttatatac cttccgatga aataagcata aaaaatgagg aataccactg ggtgacagac
    11581 ggccgcgtta ttctctggcg tggcaaaatc gttagcaatc caccgggaac ccccactgtc
    11641 gatattgcca gctttcaggc tatgggccgc ttcgcggtcg ataaatatag cctctatttt
    11701 gacggacagc gcaccgaaag taatagcggc gcgtcacgtg ttgatctggc gacactaaaa
    11761 gctatcgaag gtaactctac cacgctggtg gatagtaaaa atctctattt gtccggtcga
    11821 cgtcagggga gcagcagtaa tgttactgta ttagaaaaaa gatggtgggg tattaatcca
    11881 cgtcttatga gtgtaaacag aaatttgtat tccaatgatc tgcttatccg cagtgggcag
    11941 aatatttatt taaatggcgt tcaccttacg gcgaatgcag actcatttga gataattcgc
    12001 tggatacctc attcactgct ggtttttcgc gacaataagg gtatgcatcg ttatcccttt
    12061 gggcaattgt ctggcaaagc gatacccgta gatgatgacg tctcttttga agtaggggaa
    12121 agtcgcgttc gctggcgtaa acagctcacg tccgaccgtc agtggagcaa gtggatagac
    12181 ctaccagata ttgaacctga acaattccat ctgattactg gcaatattgc gcaatataaa
    12241 gatcggctgt atgtaacaaa attatcgaca tttggtgaag accagcttga gataatcccg
    12301 ctggatacgc cagacctggt cattgatcgc tcatttaata gcggcaaaca gcatgcttat
    12361 tttatccgcc aattacggtc aaagagcgtg caaattattc cagttaacgg tccgctaact
    12421 aaaaacgatc gcttcgctta tgacgatcgc aatgtttata catggaccga tacagaggta
    12481 aggattacgc cctccccctg cccggcgaaa acacacgtca gagaagagaa tgtgcgtgaa
    12541 cttcacaaca gagacattat tattccgttg acggatgact catgccggaa cgcagcaaca
    12601 gacgggcaaa ctttgaagcc ctgaccccat accgctatac tgccgccatc acttaaagac
    12661 aggatacaac aatgaacgac agtgaatttc atcgcctggc tgatcaactc tggctgacca
    12721 ttgaagaacg cctggacgac tgggatggcg acagcgatat cgactgcgaa atcaacggcg
    12781 gcgtactgac cattaccttt gaaaacggca gcaaaatcat tattaaccgc caggagccgc
    12841 tgcaccaggt gtggctggca accaaacagg gcggctacca ttttgaccta aaaggcgatg
    12901 aatggatttg cgatcgcagc ggcgaaacct tctgggattt gctggaacag gcggcgacac
    12961 agcaagcggg tgaaacagtc agtttccgct aagattgcat gccggataag cctcgctttc
    13021 cggcacgttc atcacgaaaa atattgctgt aatagcggcg tatcgtgatc ctgattggca
    13081 ggcggcatgt taccgataga ttttgtgcgg aacggaatca cctgttcacg accatcaacc
    13141 ttcacaatct gatagaactg cggcaggttg aagttgatga agcttgagcc gtaggtgaag
    13201 cggtcgtgcg atgacgagta gaagcgactg acgtcacgca ccagttcctc tttgctgcct
    13261 tcgcagtggt gatacacctc aacccggttg ctttcgtcga gaatgtagat attaaagcca
    13321 ttctcgtctt gcgtttcttc gaagaagaac tggatgatcc cttcactagc aaagccgtcc
    13381 accaccgccg gtaatttgac gtgattggtt tcaacctgca ctgacaagcc gtgcagtttg
    13441 ttatgcgaaa tagcgccata aaactcgatg gcgttttcca gtttttgtac cgatacattc
    13501 aggcgttcga agaacaaccc ccaggtttga ccagaaacgc gcagcgcctt gaaacgcccg
    13561 gtttcctggc gggtgctgga aaggcgcagt tcaatacact cagaaaccag ttgctgcacg
    13621 cgagtacgga ttaacccgcg cagatgctgg ctataacaga agacttccac gctatctggc
    13681 ggtgcggcgt cctgatgcat tttgccgaga atagttttca gggcttcgat catcgattgc
    13741 tcgccgttga agtgcagcgt acgcacttcg ttccacgagt tgcggtacag caggtcaacg
    13801 ctacctacca ggcaattttg attctcgcca aagctgaaga catccagctt acggaaatca
    13861 aaatgcacca cctgattgcg gaacgccgct gtcgggtcat attccaggtt aacgataatc
    13921 gccagatggc ggatctcaca cgggctgtag agcgccttcg gtgtcggtgc aggtaagcgc
    13981 agcgggaaat ggtgcgacac gtcggcgacc atctcctgca acttaggcaa atcgacaatg
    14041 ccgttacctt taatatacaa acgggtgcgc gaggtcagca ggccgttaaa ccatgcccac
    14101 gccaccagtt tattcaggta acggttatat tccagcggct gatggctgat gatcgactca
    14161 atatttggcg cgcggttata cagataccaa cctgaacggt tagcacggcc cggcggcaca
    14221 taaataaagg tcagattcgg ttccgagaga tcgggtgaaa tctgcgggtt taccagcgtc
    14281 actttacctg gtaatgcttc aaacgcggca tacagcttac gcgtcagcac gccgatatcc
    14341 tgcggactgg cggagacgct aaggttattg cgacgcgcaa agcggatcag attacggtag
    14401 ctctgcatca tcgcgtcgag caactcgttg tgcgcctcac gcacctgatc aatcttccag
    14461 ttagcgcggt tatcgagcat tgccagacga gcttcgtccc aaccccactc gctcactaac
    14521 tggctcaaca ctgcgcgacg ccagcctacg caggcgcgtt cacggctgag cttttcgcac
    14581 acttttaaat agaagcagcg acgtactaaa tccagacggg taaaatcttc aatcgccgtc
    14641 aggtattcag taacacgctc cagcatcatg cagtatggat cgagaccaaa cgatacaatc
    14701 tcgccgtcgt gcaaacgctg tttgatatct ttcgccagca gacgtgggtt cgggtattcc
    14761 caggaatagg cttccagcag cagtgttttc agtaccgctt tgtatgggga atcgatactc
    14821 ttgtagagct gccaaaggct ggcaccaaag tactcttcag cagaaagcga gcttaagcca
    14881 ccgagatcca gccattcatt tggcgtcagc acgccctgcg cgtaaagcgt catcacatag
    14941 tcgtcgtaat gctcttcttc gtcgcacggc accatattcc acagaatacg cttaccggcg
    15001 agacgcacgg cggtacgata aaattcgtca agcagcagta tatgctgggt ggagccacaa
    15061 tcttcgcccc ccaggctgcc gctttcatta tgacggaagc ggttttcatc aatcaggaag
    15121 aagctgactt ccacacccag cgaggcggcc cagctttcca gcagactaca tttacgttgt
    15181 agtaactggc gctcttcgct atcgagccag gattgatgac agacccagat atccaggtca
    15241 gaggaacaac tttgccctac ggacgaggtg ctgcccatgg tgtatacacc agtaattgga
    15301 agctcacctt tcggtgggtc ctgtactgac attccacgat acagttcaag ctcgttcagg
    15361 tagtggcgtt gagtttcatc aggcgtgtaa aggcaaatgc ctttgggaac gttaccatca
    15421 aggtaacccg gcattagcgg atggtgatag tgcaacaatg tcggcagtag actgtagacc
    15481 tgttggaatg caggccccat agcagcaagc gcgcgatcca cacgcaattg atttatggca
    15541 tccagtctct gtttcagagt ctcaatatag aggtacaaga cgtatcgcct gatttgctac
    15601 ccgtcatgac tgtgattccg ccaacatcaa cggtaacacg cggcattcgg gatatttcgt
    15661 atgtcaaagg taaccgttac cacttttcgc gcctggtttt tttagtttca cgacgaaaaa
    15721 atggtctaaa acgtgatcaa tttaacacct tgctgattga ccgtaaagaa agatgcgcta
    15781 catacaagtg tagcaccgtt tattctctgt aaattcctta ttacgacggc gtgaaacgcc
    15841 tgtcaggatc cactgccaga cctcatttta cggtttgcgc aggcgtctac gtttcaccac
    15901 aacactgaca tcactctggc aaggatgtta ggatggacca cggatgataa tgacggtaac
    15961 aagcatgtta gacaatgttt taagaattgc cacacgccaa agcccacttg cactctggca
    16021 ggcacactat gtcaaagaca agttgatggc gagccatccg ggcctggtgg ttgaactggt
    16081 accgatggtg acgcgcggcg atgtgattct tgatacgccg ctggcgaaag taggcggaaa
    16141 aggcttattt gtaaaagagc tggaagtcgc gctcctggag aatcgcgccg atatcgccgt
    16201 acattcaatg aaagatgtgc cggttgaatt cccgcaaggt ctggggttgg tcactatttg
    16261 tgagcgtgaa gatcctcgcg atgcctttgt gtccaataat tatgacagtc tggatgcgtt
    16321 accggcaggc agtatcgtcg ggacgtccag tttacgtcgc cagtgccaac tggctgaacg
    16381 ccgcccggat ctgattatcc gctccctgcg cggcaacgtc ggcactcgcc tgagcaaact
    16441 agataacggc gaatacgatg ccatcattct tgccgtagcc ggactaaaac gtttaggtct
    16501 ggagtcccgc attcgcgccg cgttaccacc cgagatttct cttccggcgg taggacaagg
    16561 tgcagtcggt attgaatgcc gccttgatga tacgcgcact cgcgagctgc ttgccgcgct
    16621 gaatcaccac gaaactgcac tgcgcgttac cgcagaacgc gccatgaata cccgtctcga
    16681 aggtggatgt caggtgccaa ttggtagcta cgccgagctt attgatggtg aaatctggct
    16741 gcgtgcgctg gtcggcgcgc cggacggttc gcagattatt cgcggtgaac gccgcggtgc
    16801 gccgcaaaat gccgaacaaa tggggatttc gctggcagaa gagctactga ataacggcgc
    16861 gcgcgagatc ctcgctgaag tctataacgg agacgccccg gcatgagtat cctggtcacc
    16921 cgcccgtctc ccgctggaga agagttagtg agccgtctgc gcacactggg gcaggtggcc
    16981 tggcattttc cgctgattga gttttctccg ggtcgacaat taccgcaact tgctgatcaa
    17041 ctggcggcgc tgggggagag cgatctgttg tttgccctct cgcaacacgc ggttgctttt
    17101 gcccaatcac agctgcatca gcaagatcgt aaatggcccc gactacctga ttatttcgcc
    17161 attggacgca ccaccgcact ggcgctacat accgtaagcg gacagaagat tctctacccg
    17221 caggatcggg aaatcagcga agtcttgcta caattacctg aattacaaaa tattgcgggc
    17281 aaacgtgcgc tgatattacg tggcaatggc ggtcgtgagc taattgggga taccctgaca
    17341 gcgcgcggtg ctgaggtcac tttttgtgaa tgttatcaac gatgcgcaat ccattacgat
    17401 ggtgcagaag aagcgatgcg ctggcaatcc cgcgaggtga cgatggtcgt tgttaccagc
    17461 ggtgaaatgt tgcagcaact ctggtcgctg atcccacaat ggtatcgtga gcactggtta
    17521 ctacactgtc gactattggt cgtcagtgag cgtttggcga aactcgcccg ggaactgggc
    17581 tggcaagaca ttaaggtcgc cgataacgct gacaacgatg cgcttttacg ggcattacaa
    17641 taactctcat aacaggaagc cataatgacg gaacaagaaa aaacctccgc cgtggttgaa
    17701 gagaccaggg aggccgtgga caccacgtca caacctgtcg caacagaaaa aaagagtaag
    17761 aacaataccg cattgattct cagcgcggtg gctatcgcta ttgctctggc ggcgggcgtc
    17821 ggtttgtatg gctggggtaa acaacaggcc gtcaatcaga ccgccaccag cgatgccctg
    17881 gctaaccaac tgacggcatt gcaaaaagcc caggagagcc aaaaagccga gctggaaggc
    17941 attattaagc aacaagctgc acagcttaag caggcgaatc gtcagcaaga aacgctggca
    18001 aaacagttgg atgaagtcca acaaaaggtc gccaccattt ccggcagcga tgctaaaacc
    18061 tggctgctgg ctcaggccga ttttctggtg aaactcgccg gacggaagct gtggagcgat
    18121 caggacgtca cgaccgctgc agcgttgctg aaaagtgcag atgccagcct ggcggatatg
    18181 aatgacccga gtctgattac cgttcgtcgg gcaattaccg atgatatcgc cagcctttct
    18241 gcagtttcgc aggtggatta tgacggcatc atccttaagc ttaatcagct ttcaaatcag
    18301 gtagataacc tgcgtctggc cgataatgac agcgatggtt cgccgatgga ttccgacggt
    18361 gaagagcttt ccagttccat cagcgaatgg cgtatcaatc tgcaaaaaag ctggcagaac
    18421 tttatggaca acttcattac gattcgccgt cgtgatgaca ccgccgtacc gctgttagcg
    18481 ccaaatcagg atatctatct gcgcgaaaat attcgctctc gcctgctggt cgcagcacaa
    18541 gctgtaccgc gtcaccagga agagacttat cgccaggcgc tggagaacgt ctccacctgg
    18601 gtacgagctt actacgatac tgatgatgcc accaccaaag cgttcctcga cgaggtggac
    18661 cagttaagcc agcaaaatat ctcgatggat cttccggaaa ccctgcaaag ccaggcgatg
    18721 ctggaaaaac tgatgcagac ccgcgtgcgt aacctgctgg cacaaccggc agcggggaca
    18781 acggaagcta aacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcaccgcaa
    18841 ggtgatactc cggcagccgc gccgcaagga gaataaccat gctaaaagtg ttattactct
    18901 ttgttttgct gattgcgggg atagtggttg gcccgatgat tgccggtcat cagggttacg
    18961 tgctgatcca gactgacaac tacaatatcg aaaccagcgt cacgggcctg gcgattattt
    19021 tgattctggc gatggtagtg ctgtttgcca ttgagtggct actgcggcgg atcttccgca
    19081 caggcgcaca cacccgtggg tggtttgtcg gacgtaagcg tcgccgtgcc cgtaagcaga
    19141 ccgaacaggc gctgctgaaa ctggcggaag gcgattatca gcaagttgaa aagctgatgg
    19201 cgaaaaatgc cgatcacgcg gaacaaccgg tggtgaacta tctactggct gccgaagccg
    19261 cgcaacaacg tggtgatgaa gcacgcgcca accaacatct ggaacgcgca gcagagctgg
    19321 ccggcaatga caccattccg gtagaaatca cccgtgtacg tctgcaactg gcccgtaatg
    19381 aaaaccatgc tgcacgccac ggcgtggata agctgctgga agttacgcca cgccatccgg
    19441 aagtattacg tctggcggaa caggcgtata tccgcacagg tgcatggagt tctctgctgg
    19501 atatcatccc atcaatggcg aaagcccatg ttggtgatga agaacatcgt gcaatgctgg
    19561 aacaacaggc atggattggc ctgatggatc aggcgcgtgc cgataacggt agtgaaggtt
    19621 tgcgtaactg gtggaaaaac caaagccgga aaacgcgtca tcaggtagcg ttgcaggtgg
    19681 caatggcgga acatcttatt gaatgtgacg atcatgatac tgcccagcaa attatcatcg
    19741 atggcctgaa acgccagtac gacgatcgcc tactgctgcc gattcctcga ctgaaaacca
    19801 acaatccgga acagctggaa aaagtgctgc gccagcagat caaaaacgtc ggcgatcgcc
    19861 cgctgttgtg gagcacactg ggccagtcac tgatgaagca cggagaatgg caggaagcat
    19921 cgctcgcctt ccgcgcggcg ctgaaacaac gtccggacgc ctacgattac gcatggcttg
    19981 ccgacgcgct ggacagactg cacaagctgg aagaagctgc agcgatgcgt cgcgacggtt
    20041 tgatgttaac gttgcagaac aacccaccac agtagttcct tctcacccgg aggcaagcac
    20101 ctccgggacc tttctgatgc ataaaaaaac gcctgctctt attacggagc aggcgttaaa
    20161 acaggtctgt atgacaacaa gtgggtgctt cactcaacgt tgtgtccatg gtgtctgatg
    20221 aggcataagc gacatctgtc agtggacgat aagcaccgta aacggctctg cgtcattccg
    20281 gagtttatga ggcactaagg cgaacataag agatggaatg agcatctacc cgtttattat
    20341 gccacagaga atcgggaaat aacatccctt aacacttgtt atgagataat tctgtaatcc
    20401 tctttgcttc ctgagtaata acttcctgag tgaatattta acctgagctt gatcctacac
    20461 atacttatta tgaatgataa aattcattca attaataaca catatattaa ttgccgttaa
    20521 aactaaaaaa agcatcaata atcaacgcga tataataaac ctgccttaca tatcaactgc
    20581 gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg gataaataaa
    20641 taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg gatctcaatt
    20701 atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt tgcatggaat
    20761 tttcgttttc acccaaactt tttgttgttg ctgtcgccgc cgctcttcct ctcatggcca
    20821 gcgcagcaga taccccgtca actgccaccg cacgcaaagg ctttgccgga tacgatcacc
    20881 caaaccagta tccacagaac gctgcaattt attgaatttg cacgatcatg taggccggat
    20941 aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cacaaaatgg ccgctttttt
    21001 acttattcac caccagcaaa tgtgcctgca taatgtcact ggccgggcga ccgtgcgcca
    21061 gcaaatcagc cattgcttta agatatggcg gtagatggcg gaaataacgc tgatacccgg
    21121 cacacaaata attcagcccc ggtttgccgc tggcatcgag catgaagcgg tgtttcgggc
    21181 agcctcccca gcacgctttt aacacgttac aactgcgaca ctgcgccggt aattgcttaa
    21241 atttatcttc accaaacacc tgctgttgcg gggaatcgat catttctgca attgtttgct
    21301 gatgcatatt ccccagccga tattgcggat aaacatagtg atcgcaggcg taaacgtcgc
    21361 cgttgtgctc aacaatcacc gagcgcccac aggttggctg atgatggcaa accgcacccg
    21421 gcgcaccgac aaaattggca aacgcccatt cgatattcat cacgaaaatc ttgccgacgt
    21481 cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc accaaattca tcggggcgca
    21541 ccgaccattc cttcagttcc ccctgaatat caccaggcgc atgtagtttc agtccatcgc
    21601 tggcagctgt ttcatcagcc aggcgctcga ccaccggaat aaactggatg aattcgactc
    21661 ccgcatcgca caaaaaatca taaacctgca acggttgctg cgcgctggtg cggttgacgc
    21721 agaccagcac gttatagtcg acatgatgtt tttgcagcag cgtcagggca cgcatcacca
    21781 gcttatgcgt tggtctgcca cctttggtca cgcgatattg attgtggatc tcagccggac
    21841 cgtccagcga taacccaaca agaaaatgat tttctgccag aaatgcacac catttatcat
    21901 cgagcagcac gccgttagtc tggaagctgt tacttatctt cctgccagca ccgtatttcg
    21961 cctgtaactt tacggcacgg cggtaaaact ccagcccgag taacgttggt tcgccgccct
    22021 gccaggtaaa agccacttcg ttttgcggtt cgctggcagc gatatagtga cggacatacg
    22081 cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc gcggtaaagg gattgttttt
    22141 cgagataaaa acagtagtca cagttcagat tacaatcgga accactcggt ttcgccatca
    22201 catgaaaagc acgcgttgga acctgttgca gcatggtcac tccttgcctg aagtcatcct
    22261 tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag ttattacggg cgtttattgc
    22321 gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat
    22381 tggtcggcga gagaggattc gaacctccga cccactggtc ccaaaccagt tgcgctacca
    22441 agctgcgcta ctcgccgaaa tactgctttt tgaattttta gttcaattct ttaaagtcgt
    22501 ggtgcgaggg gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc
    22561 gcgtctacca attccgccac cttcgcattg tcacaacttc taataatggg gtggctaatg
    22621 ggattcgaac ccacgacaac tggaatcaca atccagggct ctaccaactg agctatagcc
    22681 accactacaa atcttgttac gcggtattac taccaccgca gctcaagcgc cgggactaaa
    22741 tggcgcgccc gacaggattc gaacctgaga cctctgcctc cggagggcag cgctctatcc
    22801 agctgagcta cgggcgctta gcgccgttgc gggggtggat aatacggact tcccacccct
    22861 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
    22921 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
    22981 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
    23041 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
    23101 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
    23161 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
    23221 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
    23281 atagcaatag atactgccac acccgcaact ggtacgccgt gacgggaaac tttcgccatc
    23341 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg
    23401 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
    23461 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccaatttca
    23521 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
    23581 cacagcactt tgcctacggc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca
    23641 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg
    23701 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
    23761 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
    23821 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
    23881 gccgccagat tcgccaacgc caccagcgcc actgcgatca atgcgggtat ccactgcgcc
    23941 atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacg
    24001 gccatccaca taaaccagta agaccaggcg gtgagataac caaagaacgg gctcatataa
    24061 cgatgcgcat aaacggcgaa cgaaccggta accggttcga ggaacaacat ttcgcccatt
    24121 gaacgcatga tgaaaaagac gaatagcccg gcgatgatat aggccaacaa tacggatggc
    24181 ccggcccatt tcaaggtact ggcggccccc ataaacaggc cgacgccaat ggtgcccccc
    24241 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
    24301 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
    24361 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
    24421 tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt
    24481 acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg
    24541 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
    24601 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
    24661 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
    24721 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
    24781 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
    24841 ccactggttg cgcagtttag cttcagtttt cgccagcact tcaggtttac cgcccacaag
    24901 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcagcacc
    24961 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
    25021 gccatccgca tatttaaatt cggcagcgtt aattagctcc ctgacctcag cattatcttc
    25081 aatggtcagc attttttcgg cattaatggc aaccagcgtt ccctgcttaa gatgcccgtc
    25141 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
    25201 cagcgtatag gtcggtgcca tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
    25261 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacaacagt
    25321 tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
    25381 gaatccagcc cttcacgtgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
    25441 tgcaatatcg cagccttata gcgattagtc tcgcggttgc ccagctcata cagccagtcg
    25501 aaccatttga tgatcagtcc aaccacaatc gccccgagtg ggatgaacaa cgcgccgccc
    25561 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
    25621 acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc
    25681 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
    25741 ttgtcgtagt tctgcaacag caacgccaga ttctcccatg gcgagaaggt gtcgcgagtg
    25801 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc
    25861 gccagccaga acatgcccac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
    25921 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
    25981 cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa
    26041 aacagccacg ctttgctatc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
    26101 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag
    26161 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
    26221 gcgatgccca tcaggatcac ccacgtaaga ttggtctcca cgcgattcat ggtaaacagc
    26281 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa
    26341 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
    26401 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
    26461 aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag
    26521 gtcagcgtgg caataaacag cgtgcagagc agccagacca caaacaggcc actgaattgc
    26581 agcagactca tgcgccctcc ccggcggcaa tcgccaacgc ccgctgccag ccttgtagat
    26641 agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
    26701 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg
    26761 tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
    26821 gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
    26881 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt
    26941 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
    27001 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
    27061 acaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt
    27121 tcccttcacg ttgccgatcg ttcgccatcg tattgagaga agggtccatc cgcgtcggga
    27181 agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcgccgc
    27241 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagtggg taaaaaagct
    27301 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
    27361 ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt
    27421 ggaagaaaaa acgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
    27481 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
    27541 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcagccagc gcgtcattga
    27601 aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
    27661 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
    27721 ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
    27781 ggcaaatacc atcaataaag tgaactggct gatttccgcc agaatataaa accgcaatgt
    27841 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac
    27901 taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcagccagat
    27961 agcaaaatca cgcagcagcc agacggtaaa actcgccgcc gccactgccg gtaagacgaa
    28021 tttcagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt
    28081 gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact
    28141 gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcga gcagtttacg
    28201 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
    28261 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
    28321 accacgtttg atcaacatga tggcggcagg aattaccacc agcgcgggaa tcagcgccag
    28381 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
    28441 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
    28501 cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac
    28561 cagcccctga tagtcggtat taccaaacaa tccctggctg attggcgcag ctgccagcac
    28621 aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac
    28681 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
    28741 gatgccagct ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
    28801 cccaagcccc gccggaccaa atgacacagc cagcaactta ccgaccagta acccggcacc
    28861 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
    28921 agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
    28981 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
    29041 actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca
    29101 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
    29161 taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac
    29221 gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt
    29281 gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg
    29341 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
    29401 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
    29461 cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt tcatggaagc
    29521 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
    29581 cctgagcggc atcttctacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt
    29641 ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga
    29701 tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa
    29761 cgatttttgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
    29821 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
    29881 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
    29941 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
    30001 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
    30061 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
    30121 gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
    30181 agcgccgttt gcatcagctc agcacctaca ccgcgtccag ccagcaggcc aattcgcgca
    30241 tctgtcgcat tgagttcccg taaagagaca taaccacgaa taccgccgga cgccgcacgt
    30301 aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
    30361 aagcgtctgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
    30421 gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta
    30481 tcactgacat tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc
    30541 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
    30601 gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg
    30661 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
    30721 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
    30781 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
    30841 aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct
    30901 gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
    30961 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg
    31021 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
    31081 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
    31141 aaactccacc acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc
    31201 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
    31261 atcgcccagc accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc
    31321 ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact
    31381 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
    31441 ttcgcggata ccggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta
    31501 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
    31561 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
    31621 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
    31681 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc aaccaagttc
    31741 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
    31801 aaaggtgatc aagtcacgat agtgcgccac gccgtgcggc ttgttcggag ccagttcttc
    31861 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
    31921 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
    31981 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
    32041 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
    32101 gttattcgag cagttggtga tcagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
    32161 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
    32221 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
    32281 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
    32341 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
    32401 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
    32461 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc
    32521 gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac
    32581 taccaccgcg tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
    32641 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
    32701 tactgctgat ggacattgtc gccattgata actttgaact gactatgatc gaccagcatc
    32761 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    32821 agttttttcg gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac
    32881 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    32941 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagcagcc
    33001 aggcaatcag ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc
    33061 acttcgcgtg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    33121 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    33181 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    33241 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    33301 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    33361 agttcgctgg cgcgggccga acaaaccggc gtcataccac caatcacgcg atcgttttta
    33421 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    33481 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    33541 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    33601 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    33661 tgatctcctt taaacggcgt aggtacagca atcagccagg catccgcttc aactggcgtc
    33721 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggt
    33781 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    33841 ccaatcacct gtttttgccg tgaggcaaac gcagctgctg taggcagccc gatgtaaccc
    33901 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    33961 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagctt
    34021 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    34081 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    34141 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    34201 aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca
    34261 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    34321 gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcgt
    34381 ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca
    34441 tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtgtcgc
    34501 tgctcatcac ctgatcacgc acccataaca gtgcatcaat gactgtatta ccagtaatga
    34561 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    34621 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    34681 aatagagatc gcccgtgcgt aaaccagcct caacgtgacc aacaggaata cgctgataaa
    34741 acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc gtgaaccagc acgacgtctg
    34801 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    34861 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    34921 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    34981 gatcttttgc caacgcatgc accaacggag ccatcttgat ggcttccggg cgcgtaccaa
    35041 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctcg
    35101 cagcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    35161 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    35221 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca
    35281 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    35341 acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct
    35401 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    35461 gcaattttca gcgcctgctc aatgctgttc atccggcggt cgtagatggc tttcgccacc
    35521 tcttcctgac gcttcacctg agctttcatc tggatggtgc gtgccgccca tgcgcctttc
    35581 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    35641 aggttattag cgtcaggcgc ggtttcggca ataagcttca cgctgtcatt gaccgcgcgg
    35701 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    35761 gctttgctgt tgcccaccat ccgctgcttg taatagtcgg tttgcagcca gaactctctg
    35821 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    35881 ggttggtcgg cagaagccat gtttgaacgg gcatccaggt tacgcaaaaa ttgctgctgc
    35941 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgcgctccac
    36001 tcttgtcgag caaaaaaagt atacgccagc gcgattaacg caaacgccag ccccatgcca
    36061 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    36121 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    36181 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    36241 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    36301 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    36361 cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac
    36421 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    36481 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    36541 taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg
    36601 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    36661 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    36721 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    36781 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    36841 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa
    36901 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    36961 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    37021 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    37081 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    37141 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    37201 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    37261 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    37321 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    37381 ccatttgata tgcaaattgc ataacatgcc agcaaattcc aggaattttc ctaacatcgc
    37441 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    37501 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    37561 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    37621 tttgcgcagg atccacattt tctgcagttc ttcctgagtc gtgagcagct cttctttacg
    37681 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    37741 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg taaataactt cgtccatttt
    37801 agaaccggta tcgataagcg ccgtcgcgat aatggtcagg ctgccgccct cttccacgtt
    37861 acgcgccgca ccgaagaagc gtttcggacg atgaagggca ttggcgtcca caccaccggt
    37921 caacacttta cctgacgccg gaacaacagt gttgtaagcg cgcgccagac gagtgatgga
    37981 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    38041 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    38101 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    38161 catcagcaca caatccgggt ggttgtaagc aatgctctga gcaatgttct gcagcagcat
    38221 agttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    38281 tgccagatcc agtacgcgag cggttaaatc ttcagtagaa ccgttaccac gttccatacg
    38341 cagacgagag tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    38401 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    38461 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    38521 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    38581 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    38641 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tacgagccag
    38701 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    38761 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    38821 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    38881 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    38941 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    39001 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    39061 gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    39121 aggaactctt tcaactgacc tttagacagt gcacccactt tggttgccgc cacttcaccg
    39181 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    39241 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    39301 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    39361 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    39421 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    39481 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    39541 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    39601 ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg
    39661 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    39721 caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    39781 catcctgcga ttgccgatcg caaggtgaat caaccacgtg ccttaattat ggcaccgacg
    39841 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc taccggcctg
    39901 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    39961 ggcgttgaca ttctgattgg cactacgggg cgtttaattg actacgccaa gcagaaccac
    40021 attaacctcg gtgccattca ggtggtggta ctggacgaag ccgatcgcat gtacgatctg
    40081 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    40141 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    40201 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    40261 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    40321 gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg
    40381 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    40441 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    40501 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    40561 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    40621 ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgagacc
    40681 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    40741 ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct
    40801 ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt
    40861 atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa
    40921 gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct
    40981 gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc
    41041 tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa
    41101 tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt
    41161 tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt
    41221 acgtcttgcc gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc
    41281 ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac
    41341 aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt
    41401 aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg
    41461 gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa
    41521 agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt
    41581 gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat
    41641 ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg
    41701 tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag
    41761 tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc
    41821 gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga
    41881 tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc
    41941 gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct
    42001 tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag
    42061 cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc
    42121 cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc
    42181 ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact
    42241 ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc
    42301 agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct
    42361 aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt
    42421 ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt
    42481 tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg
    42541 ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc
    42601 agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg
    42661 tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg
    42721 acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc
    42781 aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg
    42841 tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg
    42901 tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg
    42961 ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg
    43021 gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc
    43081 agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag
    43141 tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata
    43201 tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc
    43261 agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca
    43321 tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag
    43381 aaaaacgacg taccaccaga tattttgtat gggatgcggt tttgcatcag gaatttttca
    43441 aacacccgcg actgatggtt accgcgataa agaatggcgt aatctttgta ctgcgtttta
    43501 ttgacgaagt gatgggcgat cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta
    43561 ttcgcgctta aaacttttag ctctgtgccg taacccagtt cggagaacag acgcttttca
    43621 aagacgtgcg gattattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag
    43681 cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc
    43741 aggttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg
    43801 gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg
    43861 tcctgatact catccaccag cagatagcga attttgttct gccagcgctc gcggacttct
    43921 tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg
    43981 cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg
    44041 ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg
    44101 gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct
    44161 ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc
    44221 gccgcatact cacgcttgat gatatctagc cccagcgtgt ggaaggttga gatcatcagc
    44281 ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc
    44341 ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga
    44401 tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg
    44461 caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta
    44521 ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt
    44581 gtaagccgct aaccggttgg ctgcggatgt ttaaatcaga gagagaagac cgaaaccgga
    44641 gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt
    44701 ttcagagttt actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg
    44761 aaatactgga atgcaacttt ggtaactatc ctgttagcca gaatggacag ttcagcacca
    44821 agtattacga tggccgcatt cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca
    44881 acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact cgcgatcatg
    44941 acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttaacaat ggcctgcaat
    45001 tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc agtcgaaacc
    45061 gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca catgagttgg
    45121 ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac
    45181 aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc
    45241 cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagcccctc
    45301 cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc tgacaattat
    45361 tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag
    45421 agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc ggcaagctgg
    45481 cgaagaaaca ctctatttgc ccgtcaggca aacgcggcgg tgatttaggt gaattccgcc
    45541 agggtcagat ggttccggcg ttcgataaag tggttttctc ttgtccggta ctggagccga
    45601 ccggcccgct gcacacccag ttcggatatc acatcattaa ggtgctgtac cgcaactaat
    45661 agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg tgggagaggg
    45721 tcggggtgag ggcatcagcg cgcacttaac ccgcaacagc aatacgtttc atatctgtca
    45781 tatagccgcg cagtttctta cctacctgct caatcgcatg gctgcgaatc gcttcgttca
    45841 catcacgcag ttgcgcgtta tctaccgcgc cttccggaat agctttaccc aagtcgcccg
    45901 gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag aacagatagt
    45961 taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc ttacgggcga
    46021 tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac tcttcaatga
    46081 tgccggaatc gaccatggtt tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca
    46141 tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac tgcggcgcgg
    46201 tttcaaacgc ggttttgccg gtctcttcac gccaggtcag cagtttctta tcatcgttgg
    46261 cccagtccgc catcataccg gaagagaatt cgccggagat gatgtcgtcc atatgtttct
    46321 ggaacagcgg tgccatgatc tctttcagct gttcagaaag cgcataagca cgcagtttcg
    46381 ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc gcttcggtga
    46441 tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg ccctcttcca
    46501 ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg atggtttgct
    46561 cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg cccgcacggt
    46621 gaccaccggt tgctgccgcc catgctttag cgatcgccat accttcgcct ttcggatcgt
    46681 tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac tcttcacgca
    46741 cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatatcttta cggatctgct
    46801 cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg tctttcatca
    46861 gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg ttaaccacca
    46921 gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt tcggtcgctt
    46981 tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag gagatatcga
    47041 gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag ccgacgataa
    47101 ccactttttt atcctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc cccataaagc
    47161 gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag tagttagcca
    47221 tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt
    47281 cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca cattgcaatt
    47341 tttgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa aaccttcctg
    47401 catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt tagcccatcc
    47461 acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct atttgtgcgc
    47521 gataaccgca cggtaacgct gacggaagcg ggcgaagagt tacgcgtttt cgcccagcaa
    47581 acattgttgc agtatcagca actgcgccac accatcgatc agcaagggcc gtcgctctct
    47641 ggcgagttac atattttctg ctcggtgacc gccgcttaca gccatctacc gccgattctc
    47701 gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg tgatgcggca
    47761 gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg gaaaccggag
    47821 acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt gctgattgcc
    47881 cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga ctggtcaacg
    47941 gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact gtggtttcga
    48001 cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga agcgatggta
    48061 tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct ggaaaacagc
    48121 ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa aacgccgttt
    48181 gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga ggcgttctgg
    48241 aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa gcgaagcgct
    48301 atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat tggtttcgtc
    48361 gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat ggtcgccgag
    48421 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    48481 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    48541 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    48601 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    48661 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    48721 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    48781 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    48841 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    48901 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    48961 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcgcgcac
    49021 atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg tgatgatgtc
    49081 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgctacgcc
    49141 ttcagcaaat agccctacgc gcggcagatc aaccggatga cccgcatcca gcgctgcttt
    49201 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca tcagttgttt
    49261 gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    49321 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    49381 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    49441 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    49501 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    49561 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    49621 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    49681 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    49741 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    49801 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    49861 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    49921 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    49981 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    50041 aactgaatgc cacggttcgg aatgtcgatg gcgatcaggt cgccgtcttc aatcaggcca
    50101 atgttgccgc cgcttgccgc ttccggtgag acgtggccga tagaaaggcc agaggtgccg
    50161 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagccccat tgatttcagg
    50221 aagctggttg ggtagagcat ttcctgcatc cccggaccgc ctttcgggcc ttcgtagcgg
    50281 ataacaacca catctccggc gacaacttta ccgccgagaa tcgcttctac cgcatcgtcc
    50341 tggctttcgt atactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    50401 gttttaacga tgcagccgtt ttccgcgaaa tttccgtaga gcaccgccag gccaccgtct
    50461 ttgctgtagg cgtgttccag cgagcggata cagccattgg cgcgatcgtc gtccagcgta
    50521 tcccaacggc aatcttgcga gaatgcctgg gtggtacgga tacccgcagg gccggcgcgg
    50581 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagcgtttgc
    50641 ggcaacgtca ggccaagcac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    50701 tcgccaagaa taccgataac accaccagca cgatgaacat cttccatatg gtatttctgg
    50761 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttatc gatatcactc
    50821 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac ggtgttcgta
    50881 gagccgccca tcgcgatatc cagcgtcatg gcgttttcaa acgccgcctt actggcgata
    50941 ttacgcggca gtgcactatc gtcgttttgc tcgtaataac gtttggtcaa ttcaacaatg
    51001 cgtttaccgg cattgaggaa cagctgctta cggtcggcgt gggttgccag cagcgagccg
    51061 ttgcccggct gcgacagacc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    51121 atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc gctctgggag
    51181 tcagagactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag cttgatgatc
    51241 tgatcggaca gtttggtttt cccggactcc atcgggccac cggaaacaaa gatcaccgga
    51301 atattcaggc gcagagaggc catcagcatc cccggggtga ttttgtcaca gttagagatg
    51361 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    51421 tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    51481 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    51541 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    51601 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    51661 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    51721 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    51781 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga aaaggccgaa
    51841 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa cctgaatacc
    51901 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaca cttcatccgc
    51961 caggtacagg gattcgcgcg acagcacctg ctcacgtact tcaattccca gctctttcgc
    52021 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    52081 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    52141 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    52201 cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt ttggtgctgc
    52261 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    52321 cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg gcgggtttac
    52381 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattatt
    52441 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg aaaccgggaa
    52501 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    52561 cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc catagtgcag
    52621 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    52681 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    52741 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    52801 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    52861 tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca cgatgacgca
    52921 ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca ttgacctgat
    52981 gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg gcaccagcgg
    53041 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    53101 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    53161 gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt cgctgtatcg
    53221 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    53281 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    53341 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    53401 aaccgccgcc ggtaaaccaa aacccatggt acctaaaccg ctggaggtga tgaaattttc
    53461 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    53521 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gcgcgtagat
    53581 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    53641 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    53701 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    53761 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    53821 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagttcgccg ctttggtgcc
    53881 atgcattccc agcatgccca ggtagtacgg ataatctgct tcaactgcgc ccagcccttt
    53941 cagcgtgcag gtggcaggca tttttgtggt agcgagaaat tctcgtaaag caggaactgc
    54001 ctgcgccata cccacgccac caccaacgta cagcatcggt ttttgcgctt ttgccagcat
    54061 ctggcgcgct tgctcaactt cggcatgggg gaaagtcact tcgttttcaa cggtggtgaa
    54121 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    54181 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    54241 cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    54301 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    54361 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    54421 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    54481 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    54541 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    54601 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    54661 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    54721 aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat ctctaagcct
    54781 ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg ctaatcacga
    54841 ccaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct tgttcgaagg
    54901 aatgcctaaa gagttaccat aggttttgta aatagtacaa gatatttttt ggccgatttt
    54961 tatgatgcgt tt
//
LOCUS       DAZFFI010000021        54676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFFI010000021 DAZFFI010000000
VERSION     DAZFFI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.021"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..491
                     /gene="eutS"
                     /locus_tag="ACHJ12_001512"
     CDS             156..491
                     /gene="eutS"
                     /locus_tag="ACHJ12_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4989324.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            504..983
                     /gene="eutP"
                     /locus_tag="ACHJ12_001513"
     CDS             504..983
                     /gene="eutP"
                     /locus_tag="ACHJ12_001513"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4989325.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            958..1659
                     /gene="eutQ"
                     /locus_tag="ACHJ12_001514"
     CDS             958..1659
                     /gene="eutQ"
                     /locus_tag="ACHJ12_001514"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4989326.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1656..2459
                     /gene="eutT"
                     /locus_tag="ACHJ12_001515"
     CDS             1656..2459
                     /gene="eutT"
                     /locus_tag="ACHJ12_001515"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4989327.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2456..3472
                     /gene="pta"
                     /locus_tag="ACHJ12_001516"
     CDS             2456..3472
                     /gene="pta"
                     /locus_tag="ACHJ12_001516"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4989328.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3511..3804
                     /gene="eutM"
                     /locus_tag="ACHJ12_001517"
     CDS             3511..3804
                     /gene="eutM"
                     /locus_tag="ACHJ12_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4989329.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3911..4198
                     /gene="eutN"
                     /locus_tag="ACHJ12_001518"
     CDS             3911..4198
                     /gene="eutN"
                     /locus_tag="ACHJ12_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO4989330.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4210..5613
                     /locus_tag="ACHJ12_001519"
     CDS             4210..5613
                     /locus_tag="ACHJ12_001519"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO4989331.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHJ12_001520"
     CDS             5624..6460
                     /gene="eutJ"
                     /locus_tag="ACHJ12_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4989332.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGRKKAEGLY
                     AK"
     gene            6450..7637
                     /gene="eutG"
                     /locus_tag="ACHJ12_001521"
     CDS             6450..7637
                     /gene="eutG"
                     /locus_tag="ACHJ12_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4989333.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7954..9180
                     /gene="eutH"
                     /locus_tag="ACHJ12_001522"
     CDS             7954..9180
                     /gene="eutH"
                     /locus_tag="ACHJ12_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4989334.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            9177..10580
                     /gene="eutA"
                     /locus_tag="ACHJ12_001523"
     CDS             9177..10580
                     /gene="eutA"
                     /locus_tag="ACHJ12_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4989335.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10592..11953
                     /gene="eutB"
                     /locus_tag="ACHJ12_001524"
     CDS             10592..11953
                     /gene="eutB"
                     /locus_tag="ACHJ12_001524"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4989336.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPSGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11974..12861
                     /gene="eutC"
                     /locus_tag="ACHJ12_001525"
     CDS             11974..12861
                     /gene="eutC"
                     /locus_tag="ACHJ12_001525"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4989337.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12871..13530
                     /gene="eutL"
                     /locus_tag="ACHJ12_001526"
     CDS             12871..13530
                     /gene="eutL"
                     /locus_tag="ACHJ12_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4989338.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13543..14043
                     /gene="eutK"
                     /locus_tag="ACHJ12_001527"
     CDS             13543..14043
                     /gene="eutK"
                     /locus_tag="ACHJ12_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4989339.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14089..15141
                     /gene="eutR"
                     /locus_tag="ACHJ12_001528"
     CDS             14089..15141
                     /gene="eutR"
                     /locus_tag="ACHJ12_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4989340.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHJ12_001529"
     CDS             complement(15147..16046)
                     /gene="hemF"
                     /locus_tag="ACHJ12_001529"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4989341.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHJ12_001530"
     CDS             complement(16050..16919)
                     /gene="amiA"
                     /locus_tag="ACHJ12_001530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4989342.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17133..17558
                     /locus_tag="ACHJ12_001531"
     CDS             17133..17558
                     /locus_tag="ACHJ12_001531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4989343.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17545..17994
                     /locus_tag="ACHJ12_001532"
     CDS             17545..17994
                     /locus_tag="ACHJ12_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4989344.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18055..18630
                     /locus_tag="ACHJ12_001533"
     CDS             18055..18630
                     /locus_tag="ACHJ12_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4989345.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHJ12_001534"
     CDS             18726..19625
                     /gene="yfeX"
                     /locus_tag="ACHJ12_001534"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4989346.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHJ12_001535"
     CDS             complement(19683..20987)
                     /gene="pbp4b"
                     /locus_tag="ACHJ12_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4989347.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHJ12_001536"
     CDS             complement(20992..22416)
                     /gene="murP"
                     /locus_tag="ACHJ12_001536"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4989348.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHJ12_001537"
     CDS             complement(22420..23316)
                     /gene="murQ"
                     /locus_tag="ACHJ12_001537"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4989349.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23480..24337
                     /gene="murR"
                     /locus_tag="ACHJ12_001538"
     CDS             23480..24337
                     /gene="murR"
                     /locus_tag="ACHJ12_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4989350.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHJ12_001539"
     CDS             24466..25257
                     /gene="ucpA"
                     /locus_tag="ACHJ12_001539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4989351.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25415..26431
                     /gene="cysP"
                     /locus_tag="ACHJ12_001540"
     CDS             25415..26431
                     /gene="cysP"
                     /locus_tag="ACHJ12_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4989352.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26431..27264
                     /gene="cysT"
                     /locus_tag="ACHJ12_001541"
     CDS             26431..27264
                     /gene="cysT"
                     /locus_tag="ACHJ12_001541"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4989353.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27264..28139
                     /gene="cysW"
                     /locus_tag="ACHJ12_001542"
     CDS             27264..28139
                     /gene="cysW"
                     /locus_tag="ACHJ12_001542"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4989354.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28129..29226
                     /gene="cysA"
                     /locus_tag="ACHJ12_001543"
     CDS             28129..29226
                     /gene="cysA"
                     /locus_tag="ACHJ12_001543"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4989355.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29360..30271
                     /gene="cysM"
                     /locus_tag="ACHJ12_001544"
     CDS             29360..30271
                     /gene="cysM"
                     /locus_tag="ACHJ12_001544"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4989356.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30274..30642)
                     /locus_tag="ACHJ12_001545"
     CDS             complement(30274..30642)
                     /locus_tag="ACHJ12_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO4989357.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30747..31598
                     /gene="pdxK"
                     /locus_tag="ACHJ12_001546"
                     /gene_synonym="thiJ"
     CDS             30747..31598
                     /gene="pdxK"
                     /locus_tag="ACHJ12_001546"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4989358.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31640..32149)
                     /gene="crr"
                     /locus_tag="ACHJ12_001547"
     CDS             complement(31640..32149)
                     /gene="crr"
                     /locus_tag="ACHJ12_001547"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4989359.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32190..33917)
                     /gene="ptsI"
                     /locus_tag="ACHJ12_001548"
     CDS             complement(32190..33917)
                     /gene="ptsI"
                     /locus_tag="ACHJ12_001548"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4989360.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33962..34219)
                     /gene="ptsH"
                     /locus_tag="ACHJ12_001549"
     CDS             complement(33962..34219)
                     /gene="ptsH"
                     /locus_tag="ACHJ12_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4989361.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34603..35574)
                     /gene="cysK"
                     /locus_tag="ACHJ12_001550"
     CDS             complement(34603..35574)
                     /gene="cysK"
                     /locus_tag="ACHJ12_001550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4989362.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35759..36520)
                     /gene="cysZ"
                     /locus_tag="ACHJ12_001551"
     CDS             complement(35759..36520)
                     /gene="cysZ"
                     /locus_tag="ACHJ12_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4989363.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36750..37736
                     /gene="zipA"
                     /locus_tag="ACHJ12_001552"
     CDS             36750..37736
                     /gene="zipA"
                     /locus_tag="ACHJ12_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4989364.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37807..39822
                     /gene="ligA"
                     /locus_tag="ACHJ12_001553"
     CDS             37807..39822
                     /gene="ligA"
                     /locus_tag="ACHJ12_001553"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4989365.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39824..40042
                     /locus_tag="ACHJ12_001554"
     CDS             39824..40042
                     /locus_tag="ACHJ12_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4989366.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(40039..41037)
                     /locus_tag="ACHJ12_001555"
     CDS             complement(40039..41037)
                     /locus_tag="ACHJ12_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4989367.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARSDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            41127..42053
                     /locus_tag="ACHJ12_001556"
     CDS             41127..42053
                     /locus_tag="ACHJ12_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4989368.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(42092..42856)
                     /locus_tag="ACHJ12_001557"
     CDS             complement(42092..42856)
                     /locus_tag="ACHJ12_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO4989369.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            43105..43938
                     /gene="xapA"
                     /locus_tag="ACHJ12_001558"
     CDS             43105..43938
                     /gene="xapA"
                     /locus_tag="ACHJ12_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO4989370.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            43998..45254
                     /gene="xapB"
                     /locus_tag="ACHJ12_001559"
     CDS             43998..45254
                     /gene="xapB"
                     /locus_tag="ACHJ12_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO4989371.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            45307..45465
                     /locus_tag="ACHJ12_001560"
     CDS             45307..45465
                     /locus_tag="ACHJ12_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO4989372.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            45506..46390
                     /gene="xapR"
                     /locus_tag="ACHJ12_001561"
     CDS             45506..46390
                     /gene="xapR"
                     /locus_tag="ACHJ12_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO4989373.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(46499..46574)
                     /locus_tag="ACHJ12_001562"
     tRNA            complement(46499..46574)
                     /locus_tag="ACHJ12_001562"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46539..46541),aa:Lys,seq:ttt)
     gene            complement(46579..46654)
                     /locus_tag="ACHJ12_001563"
     tRNA            complement(46579..46654)
                     /locus_tag="ACHJ12_001563"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46619..46621),aa:Val,seq:tac)
     gene            complement(46701..46776)
                     /locus_tag="ACHJ12_001564"
     tRNA            complement(46701..46776)
                     /locus_tag="ACHJ12_001564"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46741..46743),aa:Val,seq:tac)
     gene            complement(46821..46896)
                     /locus_tag="ACHJ12_001565"
     tRNA            complement(46821..46896)
                     /locus_tag="ACHJ12_001565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46861..46863),aa:Val,seq:tac)
     gene            47155..48570
                     /gene="gltX"
                     /locus_tag="ACHJ12_001566"
     CDS             47155..48570
                     /gene="gltX"
                     /locus_tag="ACHJ12_001566"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4989374.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(48622..49014)
                     /locus_tag="ACHJ12_001567"
     CDS             complement(48622..49014)
                     /locus_tag="ACHJ12_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4989375.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(49016..49375)
                     /locus_tag="ACHJ12_001568"
     CDS             complement(49016..49375)
                     /locus_tag="ACHJ12_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4989376.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            49596..49671
                     /locus_tag="ACHJ12_001569"
     tRNA            49596..49671
                     /locus_tag="ACHJ12_001569"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49629..49631,aa:Ala,seq:ggc)
     gene            49711..49786
                     /locus_tag="ACHJ12_001570"
     tRNA            49711..49786
                     /locus_tag="ACHJ12_001570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49744..49746,aa:Ala,seq:ggc)
     gene            49994..52183
                     /locus_tag="ACHJ12_001571"
     CDS             49994..52183
                     /locus_tag="ACHJ12_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HFO4989377.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVKS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(52233..53435)
                     /gene="nupC"
                     /locus_tag="ACHJ12_001572"
     CDS             complement(52233..53435)
                     /gene="nupC"
                     /locus_tag="ACHJ12_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4989378.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            53771..>54676
                     /locus_tag="ACHJ12_001573"
     CDS             53771..>54676
                     /locus_tag="ACHJ12_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4989379.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSS"
BASE COUNT        12816 a        13710 c        15088 g        13062 t
ORIGIN      
        1 attcatgcgt tgcatatgaa agtttatgca ccacagcgaa tacctctcca ttcttagtga
       61 tctacctcac cttttaaacg cgcttgccga attttgttat ttactctgac gaaaaattgt
      121 cacgatacac gaaagttttt cacaggcggc gactcatgga taaagaacgc atcattcagg
      181 aatttgtgcc cggcaaacag gtcacactgg cgcatctcat cgcgcatccc ggtgaggaac
      241 tggcgaagaa aatcggcgtt ccggatgcgg gcgcaattgg cattatgacg ctaactcccg
      301 gcgaaacggc gatgatcgcc ggtgatttag cgctgaaagc cgctgatgtg catatcggtt
      361 ttctcgaccg cttcagcggt gcattggtga tttatggctc ggtgggcgca gtagaagaag
      421 cgttatcaca aaccgtcagc ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga
      481 ccaaaagtta atacgaggca ttcatgaaac gaattgcgtt tgtgggtagc gtcggggcag
      541 gaaaaacaac gttatttaat gcgttgcagg ggaattatac cctcgccaga aaaacacagg
      601 ccgtggaatt taatgataag ggcgatattg atacgccggg tgaatatttt agccatcccc
      661 gctggtatca cgccttaatt accacgctac aggatgtaga catgctgatc tatgtccatg
      721 gcgcaaatga cccggaaagt cggttgcctg ccgggttgct ggatattggc gtcagcaaac
      781 ggcaaatcgc cgtcatcagt aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa
      841 agttattgct tgagacgggt ttcgaagagc cgattttcga gctaaacagc cacgacccgc
      901 aaagcgtgca gcaactggtg gattatctgg catctctcac caaacaggag gaagcaggtg
      961 aaaaaactca tcacagcgaa tgatattcgt gaagcacacg cgcgcggcga acaggcaatg
     1021 tccgtggttc tgcgcgccag cattattacc ccggaagccc gtgaagtggc ggatctactg
     1081 ggttttacca tcaccgaatg tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc
     1141 gtgccagccg acaaaaccga aagccagcgc atccgcgaaa ccatcattgc ccagctgccg
     1201 gaaggccagt ttaccgaaag cctggtcgca cagctgatgg aaaaagtgat gaaggaaaaa
     1261 cagtcgctgg agcagggcgc aatgcagccg agcttcaaat cggtgaccgg caaaggcggc
     1321 atcaaagtga ttgatggcag cagcgtcaag tttggtcgtt tcgacggcgc agagccgcat
     1381 tgcgtgggct tgactgatct tgtgaccgga gacgacggca gcagcatggc cgctggtttt
     1441 atgcagtggg agaacgcatt cttcccgtgg accctgaact acgacgaaat cgacatggtg
     1501 ctggaagggg agctgcatgt tcgccacgaa ggcgaaacca tgatcgccaa agcgggtgac
     1561 gtgatgttta tcccgaaagg ctccagtatc gaatttggta cgacatccag cgtgaaattc
     1621 ctgtatgtcg cctggccggc taactggcaa tccctatgaa agatttcatc accgaagcat
     1681 ggctaagagc gaaccatacg ctcagcgaag gcgcagagat ccatctccct gcggacagtc
     1741 gcctgacgcc ctctgcccgg gagttactgg aaagccgcca tctgcgcatc aagtttattg
     1801 acgagcaggg ccgcctgttt gttgacgatg aacagcagca gccgcagccc gttcatgggc
     1861 tgaccagtag cgatgaacat ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa
     1921 aaaagccgga tacgctgact cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc
     1981 gtctgggttt tcgcgcggtt ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg
     2041 aactggcgga accgtggcag ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta
     2101 tgcgcgccga tgcgctgggt gaaccgctgg gctgccaggc gattgtcggg ctttctgacg
     2161 aagatctgca tcgactttct caccagccgc tgcgctatct cgaccacgat catctggtgc
     2221 cggaagccag ccacgggcgc gatgcggcgt tgctcaatct gctacgtacc aaagtgcggg
     2281 aaaccgaaac cgtagcggcg caggtgttta tcacccgcag ctttgaagta ttacgcccgg
     2341 acattttgca ggcgctgaac cgcctttcca gcacggtcta cgtaatgatg atcctgagcg
     2401 taacgaagca gccgctaacg gtgaagcaga tccaacaacg actgggggaa acgcaatgat
     2461 tattgaacgt tgtcgtgaac tggcgttgcg agcgcccgcc agagtggttt ttccggatgc
     2521 gttagaccaa cgtgtgctga aagccgcgca atatttacat caacaaggtc tggcaacgcc
     2581 cattctggtc gccaatccgt ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat
     2641 ggacgggcta caggtgattg atccgcatgg caacctcgca atgcgggaag aatttgctca
     2701 tcgctggctg gcccgcgcgg gcgaaaaaac gccgccggat gcgctggaaa aacttaccga
     2761 ccctctgatg ttcgccgcag caatggtcag cgccggtaaa gcggatgtct gtatcgcggg
     2821 caacctctct tccacggcaa atgtgctgcg tgccggatta cgcattatcg gcttgcagcc
     2881 aggctgtaaa acgctctctt ccattttcct gatgttgcca cagtacagcg gcccggcgtt
     2941 gggctttgcc gattgcagcg tggtgccaca gccgacggcg gcgcagctgg cggatatcgc
     3001 gcttgccagt gccgaaacct ggcgcgccat caccggagaa gagccgcgcg tggcgatgct
     3061 gtcgttttcc agcaatggta gcgcccgtca cccctgtgtt gctaacgtcc agcaggcgac
     3121 agaaatcgtc cgtgagcgcg caccaaagct ggtggtggat ggcgagttac agtttgacgc
     3181 cgccttcgtg ccggaagtgg cggcgcaaaa agcgcctgcc agcccgctac agggcaaggc
     3241 caatgtgatg gtttttccgt cgctggaagc cggaaatatt ggttacaaaa tcgcacaacg
     3301 actcggcgga tatcgtgccg tcgggccact gatacaagga cttgccgcgc cgatgcacga
     3361 tctctctcgt ggttgtagcg tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc
     3421 gcgtcagaca gaagtgaacc gcgaaagcag tttacaaaca ctggttgaat gaacggtccc
     3481 gttctggacc ctgataaaga ggaaagcaca atggaagcat taggaatgat cgaaacccgg
     3541 ggcctggttg cactgattga ggcttctgac gcgatggtga aagctgcgcg cgtcaagctg
     3601 gttggtgtta agcagattgg cggcggcctg tgtactgcca tggttcgtgg tgatgttgca
     3661 gcctgtaaag ccgcgaccga tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta
     3721 tctgtacacg ttatcccgcg tccgcacggc gacctggaag aagtcttccc gatcggcctg
     3781 aaaggcgata gcagcaacct gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct
     3841 ctcctttttc attttacctt ctgcggaggg tagggggagc ttttacctga aatacgccac
     3901 ggaggcgggt atgaaactgg cagtcgtcac tggacaaatt gtttgtaccg tacgccatca
     3961 cggactggcg catgacaaat tgctgatggt ggaaatgatt gatccacaag gtaatcctga
     4021 cgggcaatgc gccgtcgcca tcgacaatat tggcgcggga accggggagt gggtgttact
     4081 ggtgagtggc agttccgccc gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg
     4141 cgtgattggc attgtcgatg aggtggtgtc tggcggtcag gtaattttcc acaaataagg
     4201 cagaacatca tgaatcaaca ggatattgaa caggtggtga aagcggtact gctgaaaatg
     4261 caaagcagtg acacgccgtc cgccgccgtt catgagatgg gcgttttcgc gtccctggat
     4321 gacgccgttg cggcagccaa agtcgcccag caagggttaa aaagcgtggc aatgcgccag
     4381 ttagccattg ctgccattcg tgaagcaggc gaaaaacacg ccagagattt agcggaactt
     4441 gccgtcagtg aaaccggcat ggggcgcgtt gaagataaat ttgcaaaaaa cgtcgctcag
     4501 gcgcgcggca caccaggcgt tgagtgcctc tctccgcaag tgctgactgg cgacaacggc
     4561 ctgaccctaa ttgaaaacgc accctggggc gtggtggctt cggtgacgcc ttccactaac
     4621 ccggcggcaa ccgtaattaa caacgccatc agcctgattg ccgcgggcaa cagcgtcatt
     4681 tttgccccgc atccggcggc gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag
     4741 gcgattgttg ccgcaggcgg gccggaaaac ttactggtta ctgtggcaaa tccggatatc
     4801 gaaaccgcgc agcgtttgtt caagtttccg ggcatcggcc ttctggtggt gaccggcggc
     4861 gaagcggtag tagaagcggc gcgtaaacac accaataaac gtctgattgc cgcaggcgct
     4921 ggcaacccgc cggtagtggt ggatgaaacc gccgacctcg cccgtgccgc tcagtccatc
     4981 gtcaaaggcg cttctttcga taacaacatc atttgtgccg acgaaaaggt actgattgtt
     5041 gttgatagcg tagccgatga actgatgcgt ctgatggaag gccagcacgc ggtgaaactg
     5101 accgcagaac aggcgcagca gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga
     5161 aaaggcaccg tcagccgtga ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc
     5221 ggcctgaacg tgccgcaaga aacgcgtttg ctgtttgtgg aaaccactgc agaacatccg
     5281 tttgccgtga ccgaactgat gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg
     5341 gatgccattg cgctggcggt gaaactggaa ggcggttgcc accacacggc ggcaatgcac
     5401 tcgcgcaaca tcgaaaacat gaaccagatg gcgaatgcta ttgataccag cattttcgtt
     5461 aagaacggac cgtgcattgc cgggctgggg ctgggcgggg aaggctggac caccatgacc
     5521 atcaccacgc caaccggtga aggggtaacc agcgcgcgta cgtttgtccg tctgcgtcgc
     5581 tgtgtattag tcgatgcgtt tcgcattgtt taaggagtaa cagatggcgc acgacgaaca
     5641 atggctcacc ccacgtctgc aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac
     5701 ggaatctccg ctgtggctgg gggtggatct gggcacctgc gatgtggtgt cgatggttgt
     5761 cgaccgcgac ggtcagccgg tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg
     5821 catcgtctgg gatttcttcg gcgctgtcac cattgttcgt cgccatctcg acacgctcga
     5881 gcagcaattc ggtcgccgtt ttagccatgc ggcgacttca tttcctccag gtacggaccc
     5941 gcgtatttcc atcaacgtgc tggaatctgc cgggctggaa gtgagccatg tgctggatga
     6001 gccaaccgca gtcgcggatc tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg
     6061 cggcactacc ggcattgcca tcgtgaaaaa gggcaaggtg acgtactcgg cggatgaagc
     6121 caccggcgga catcacatct ctctgactct cgccggaaat cgtcgtattt cgctggaaga
     6181 ggcagagcag tacaagcgcg gtcatggtga cgagatttgg cccgcggtga aaccggtgta
     6241 cgaaaaaatg gcggacattg tcgcccggca tattgaaggg cagggcatta ctgatttatg
     6301 gctggcgggc ggctcttgta tgcaaccggg cgtggcggaa ctgtttcgca aacaattccc
     6361 ggcgttacag gtgcatttac cgcagcacag tttgtttatg accccgctgg cgatcgccag
     6421 tagtgggcga aagaaagcgg aggggctata tgcaaaatga attgcagacc gcgctctttc
     6481 aggcgttcga taccctgaat ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc
     6541 tttgcggtcc gggctcggtg agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac
     6601 atctgttcgt gatggcagac agctttttgc atcaggcagg gatgaccgcc gggctgacgc
     6661 gcagcctggc tgttaaaggc atcgccatga cgctctggcc atgtccggtg ggcgaaccgt
     6721 gcatcaccga cgtgtgtgca gccgtggcgc agttgcgtga gtcaggctgt gatggggtga
     6781 tcgcatttgg cggcggctcg gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga
     6841 acccggatag cacgctggcg gaaatgtcag aaaccagcgt tctgcaaccg cgcctgccgt
     6901 tgattgccat tccaacgacc gccggaaccg gctctgaaac caccaacgta acggtgatta
     6961 tcgatgcggt gagcgggcgc aagcaggtgt tagcccatgc ttcgctgatg ccggatgtgg
     7021 cgattctcga cgccgcattg accgaaggtg tgccgtcgca tgtcacggcg atgaccggca
     7081 ttgatgcgtt aacccatgcc attgaagcat acagcgccct gaacgctaca ccgtttaccg
     7141 acagtctggc gattggtgcc attgcgatga ttggcaaatc gctgccgaaa gcggtgggct
     7201 acggtcacga ccttgccgcg cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg
     7261 cgttttccag tgcgggtctt gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc
     7321 tgcatattcc acacggtctg gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc
     7381 ggatggtttg tcgcgaacgg tttagtcaga ttggtcgggc actgcgaact aaaaaatccg
     7441 acgatcgtga cgctattaac gcggtaagtg agctgattgc ggaagttggg attggtaaac
     7501 gactgggcga tgttggcgcg acatctgcgc attacggtgc atgggcgcag gccgcgcagg
     7561 aagatatttg tctgcgcagc aacccgcgta ccgccagcct ggaacagatt gtcggactgt
     7621 acgcggcggc gcaataaatg ccggatgcga cgcttgccgc gtcttatccg gcctacggga
     7681 ttgcacatgt agggcggata aggcgtttac gccgcatccg ccagtggcgc ggtgcaattg
     7741 ccggatgcga tgcttgccgc gtcttatccg gcctacggga ttgcacatgt agggcggata
     7801 aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga cgcttgccgc
     7861 gtcttatccg gccttcggga ttgcacatgt agggcggata aggcgtttac gccgcatccg
     7921 ccaataaata acaactaaca gggagtaaag gcgatgggaa ttaacgaaat catcatgtac
     7981 atcatgatgt tctttatgct gatagctgcc gtagacagga tcctgtcgca gttcggcggt
     8041 tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa
     8101 gaaggcttta tggcaatggg cgctctgggt ctggcgatgg tcggtatgac cgcgctggca
     8161 ccggtactgg ctcacgtact cgggccggta attatcccgg tttacgaaat gctcggcgca
     8221 aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata tgggcggttt cttcctcgcc
     8281 aaagagctgg cgggcggcga cgtagcagcg tggctatact ctgggttaat tcttgggtcg
     8341 atgatggggc caacgattgt gttttccatt ccggtggcgc tcggcattat cgaaccttct
     8401 gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg tgaccattcc gattggctgt
     8461 attgctggtg gtctggttgc tatgtactcc ggtgtgcaga tcaacggtca gccggtggaa
     8521 ttcactttcg ccctgatcct gatgaacatg atcccggtga tcattgtcgc gattctggtg
     8581 gcgctggggc tgaaattcat cccggaaaaa atgatcaacg gcttccagat cttcgccaaa
     8641 ttcctcgttg cattgatcac cctcggtctt gccgctgcgg tagtgaaatt cctgcttggc
     8701 tgggaactga tccccggtct ggatcctatc tttatggccc ctggcgataa acccggtgaa
     8761 gtgatgcgcg ccattgaagt tatcggttct atctcctgcg tgctgttagg ggcgtatcca
     8821 atggtgctgc tgctgactcg ctggtttgaa aaaccgctga tgagcgtcgg taaagtactg
     8881 aatatgaaca acatcgcggc agccggcatg gtggcaacgc ttgccaacaa catcccgatg
     8941 ttcggcatga tgaagcagat ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt
     9001 tccgctgctt tcgccctggg cgaccactta ggcttcgccg ctgccaacat gaacgccatg
     9061 atcttcccga tgattgtcgg caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg
     9121 atgctggtgc caaaagaaga cgcgaccgcg actaaaaccg aagcggaggc acaatcgtga
     9181 acactcgcca gctattgagc gtcggtatcg atatcggcac caccaccacc caggtgattt
     9241 tctcccacct ggagctggtt aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca
     9301 ttaaacgcga aattagctgg caaagtccgg tgttctttac ccctgtcgat aaacagggcg
     9361 gtttaaaaga agcggaactg aaaaccttaa tactcgagca atatcaggct gcgggtattg
     9421 cgccggaaag cgttgattct ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca
     9481 atgctcgccc ggcggtgatg gcgctctctc aatcgctggg ggattttgtc gttgccagcg
     9541 ccgggccgca cctcgaatcc gtgatcgccg gtcacggagc tggggcgcaa accctttctg
     9601 aacaacggct gtgtcgggta ctgaatatcg acatcggcgg tggcaccgcg aactacgccc
     9661 tgttcgatgc cggaaaaatc agcggcactg cctgtctcaa cgtcggtggt cgcctgctgg
     9721 aaaccgacag ccaggggcgc gtggtttacg ctcataaacc ggggcagatg attgtggatg
     9781 agtgcttcgg tgcaggcact gacgcccgtt cgctgaccgg cgcgcagctg gtgcaggtta
     9841 cccggcggat ggcggcgctg attgtcgaag tgattgacgg aacgctttcg ccgctcgcgc
     9901 aggcattgat gcaaaccggt ttgctgcccg caggtgttac gcccgaaatc attacgcttt
     9961 ctggaggcgt gggcgaatgt tatcgccacc agcccgccga cccgttctgt tttgccgata
    10021 ttggcccact tctggcaacg gcgctgcatg accatccgcg cctgcgtgag atgaatgtgc
    10081 agtttccggc gcaaaccgta cgcgccacgg tgattggcgc gggtgcacat accctttcgc
    10141 tctctggcag cacaatctgg ctggagggcg tacaactgcc gctgcgcaat ttgccggtgg
    10201 cgatcccgat tgatgaaacg gatctggtga gtgcctggca acaggcgctg cttcagctgg
    10261 atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt
    10321 acgccgcggt actgacggtc atcaacgcgc tggtcgattt cgtcgcgcgt tttccgaatc
    10381 cgcatcccct gctggtggtg gccgggcagg actttggtaa agctctgggc atgttgttgc
    10441 gcccacagct acaacaactc ccgttggcag tcattgacga agtgattgtc cgcgcggggg
    10501 actatatcga cattggtacg cctctttttg gcggatcggt tgtgccggtg acggtgaaat
    10561 cactcgcatt tccttcctga gggaacgact tatgaaacta aagaccacat tgttcggcaa
    10621 tgtatatcag tttaaggatg taaaagaggt gctggctaaa gccaacgaac tgcgttcggg
    10681 ggatgtgctg gcgggcgttg cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt
    10741 gttgtcggaa atgaccgtag cggacatccg caataatccg gtgattgcct atgaagatga
    10801 ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc agattaaaaa
    10861 ctggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaaccagcg tggacgacat
    10921 tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga agatttgctc
    10981 caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg ccaataccac
    11041 cattggtatt ccgggcacct tcagcgcccg tttgcagccg aacgataccc gtgacgacgt
    11101 gcaaagtatc gctgcgcaaa tctatgaagg gctttccttt ggggtgggcg atgcggtgat
    11161 cggcgttaac ccggtaactg acgacgtgga aaacttaagc cgcgtgttgg acaccattta
    11221 cggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc acgtcaccac
    11281 ccagatcgaa gcgattcgtc gcggcgcgcc tagcggactg attttccaga gtatctgtgg
    11341 cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc
    11401 agtgggtgca gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg aaaccggaca
    11461 aggctctgcg ctatccgctg gcgctaactt cggcgctgac caggtgacga tggaagcacg
    11521 taactatggg ctggcgcgtc attacgatcc gtttattgtc aacaccgtgg tcggctttat
    11581 tgggccggag tatctctaca acgaccgcca gattatccgt gctggcttag aagatcactt
    11641 tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgttacacca accacgctga
    11701 cgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag gctgcaacta
    11761 catcatgggg atgccgctgg gtgatgacat catgctcaac taccagacca ccgcattcca
    11821 cgataccgcc actgtgcgtc agttactcaa cctgcgtccg tcaccggagt ttgaacgctg
    11881 gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc
    11941 actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat tgaagaaatt
    12001 gtacgcagcg tgatggcgtc aatgggacaa acggcccccg cgccgtcaga agcaaagtgc
    12061 gccaccacca actgtgcggc accggtgacc tcggaaagct gtgcgctgga tttaggttcc
    12121 gctgaagcaa aagcgtggat tggtgttgaa aacccgcatc gcgcagacgt attaacagaa
    12181 ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgtg ccggtccgcg tccacgtacc
    12241 caggcgctgc tgcgtttcct ggccgatcac tctcgttcga aagataccgt actgaaagaa
    12301 gtgccggaag agtgggtgaa agcgcagggc ttgctggaag tacgctctga aattagcgac
    12361 aaaaatctct acctgactcg cccggatatg ggccgccgcc tgtgtgcaga agctgttgaa
    12421 gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg tcgttatttc tgatggcctg
    12481 tcaactgatg ccatcaccgt caactacgaa gagatcctgc cgccgctgat ggcgggcctg
    12541 aaacaggccg ggctgaaagt cggcacgcca ttctttgttc gttatggtcg cgtgaagatt
    12601 gaagatcaga tcggtgagat cctcggcgcg aaagtggtga tcctgctggt gggggaacgt
    12661 ccggggttgg gtcagtcaga aagcctctcc tgttacgccg tttactcgcc gcgtatggcg
    12721 accaccgttg aggccgatcg cacctgcatc tctaacattc accagggcgg cacgccgccg
    12781 gttgaagccg cagccgtcat tgtggatttg gcgaaacgta tgctggagca gaaagcatcc
    12841 ggcatcaaca tgacccgata aggaggcatc atgccagctt tagatttgat tcgaccgtcg
    12901 gtaaccgcca tgcgggtgat tgcctccgtt aacgctgaat ttgcgcgtga actaaaattg
    12961 ccgccgcata ttcgtagcct cgggctgatt tctgctgatt ccgatgacgt cacctacatt
    13021 gccgctgatg aagcgaccaa gcaggcgatg gtggaagtgg tatatggtcg ctcgctgtat
    13081 gcgggcgctg cacacggccc gtcaccgact gccggtgaag tgctgattat gcttggtggg
    13141 ccaaacccgg cggaagtgcg tgccggtctg gatgcgatgg ttgcacatat tgaaaatggt
    13201 gcagcattcc agtgggccaa tgacgcagaa aacaccgcct tcctggcgca tgtggtttcg
    13261 cgtaccggtt cttatctctc atcaaccgcc gggatcacgc ttggcgatcc gatggcatat
    13321 ctggtggcac cgccgctgga agcgacctat ggcattgatg cagcgttgaa atctgccgac
    13381 gtacagctgg tgacctatgt cccgccaccg tctgaaacca actactcggc agcattttta
    13441 accggtagcc aggccgcgtg taaagcagcc tgtaacgcct ttaccgatgc agtgctggaa
    13501 atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc cgatgatcaa tgcactggga
    13561 ttgctggaag tggacggcat ggtcgccgcg atagatgcag cggatgccat gctcaaagca
    13621 gctaacgttc gtctgctcag tcacgaagtg cttgaccctg gtcgcttaac gctggtggtg
    13681 gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag gttgtgctgc cgcgatgcgt
    13741 accgggcgtg tcatcagccg caaggagatc ggtcggccag acgatgacac ccagtggctg
    13801 gttactggct ttaaccgcca gccgaagcaa cccgtaaggg aacccgacgc gccagttatc
    13861 gtcgcggaat ctgctgacga gttgttggcg ctgttaacat cagtacgtca gggaatgacg
    13921 gcaggagaag tggctgccca ctttggctgg ccgctggaaa aagccagaaa tgcgctcgaa
    13981 cagctctttt ctgccgggac gttacgtaaa cgcagtagtc gttatcgtct caagccccat
    14041 taacctgtcg gaggtgccgg gtgtcctaca acacccggca ttaacatcat gaaaaagacc
    14101 cgtacagcca atttgcacca tctttatcat gaacccttac ccgaaaacct gaagctcacg
    14161 ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa cggatgtcta tgaacatgct
    14221 ttaacgatta ccgcctggca gcagatttac gatcagctgc atccgggcaa gtttcatggt
    14281 gaatttacgg aaattctact cgatgatatt caggtttttc gtgaatacac cggtctggcg
    14341 ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt ttggcattcc ggcgacgcgc
    14401 ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg cggaaatcgc cacccgccct
    14461 ggtggcactg aatttgaact gagcacgccg gatgattaca cgatcctggg cgtggtgctt
    14521 tctgaagatg tcatcacccg gcaggctaac tttttgcata acccggatcg ggtattacat
    14581 atgctgcgta gccagtcggc gctggaagtg aaagagcagc ataaagccgc gctgtggggc
    14641 tttgtccaac aggcgctggc gacgttttgc gagaacccgg aaaatctcca tcagcctgca
    14701 gtgcgaaaag tgctggggga taatttgcta atggcgatgg gggcgatgct ggaagacgcg
    14761 caaccaatgg tgacggcgga aagcatcagt catcagagtt accgtagatt actttcccgc
    14821 gcccgtgaat atgtgctgga aaatatgtct gagccggtga cggtgctgga cttgtgtaat
    14881 caactgcatg tcagtcgccg cacgctacaa aacgcgtttc acgctatttt aggcattggc
    14941 ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac gccgcgaact gataagcccg
    15001 tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt ggggattctg gcatctgggg
    15061 caatttgcca cggattacca gcagctgttt gccgagaagc cgtcgttgac gttgcatcag
    15121 cggatgcggg aatgggggtg agggagttac acccaatccc tgaccttaat aaactcactt
    15181 aacgccgctt ctgggctgcc atcttttggc tgataatcat actcccagcg taccagtggc
    15241 ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt gcaggccaaa cagagtgccg
    15301 cgatcccaga ccagattgaa ctcgacataa cgaccgcgac ggtagagctg aaaattgcgc
    15361 tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa ttggtaaata agcgtcggtg
    15421 tagcctttgc ctaccgcctg cataaaggca aaacagtggt cgaaatctgg cgtgttcaga
    15481 tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc gatgtttgag gtagaagtaa
    15541 tcgtcgcacc actttttgta acggggataa acgtcttcac caaatggcag gcacaggtca
    15601 cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac catagaaagg ggttaaatcg
    15661 aagccgccgc caaaccacca gacgggctct gcacccggtt tttcggcaat aaaaaaacgc
    15721 acattcgcgt ggctggtggg aacatacggg ttatgcggat gcactaccag tgaaacgccc
    15781 atcgcctcga aactgcgccc ggcaagctcc gggcgatgag cggtggcgga agcaggcatc
    15841 gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt cgaaaacacc accattacgc
    15901 aacacccgac tacgcccgcc gccgccagct tcgcgctgcc aactatcttc gacaaattct
    15961 gcgccatcga cggcggtcag ctgctgacaa atcgtatcct gaaggttgag cagaaactgt
    16021 ttaacctggt gtgcgtcggg tttcataact tatcgctttt tcgaatgtgc tttctggttg
    16081 tcgaaccagt ggaaataact gatcacgcct tcagcaatcg ctgtggcgat tttctgacga
    16141 aacgccgccg tgcctaacag ccgctcttct tccgggttgg tgataaacga ggtttccacc
    16201 agcaccgaag gaaccgacgg cgatttcaac accacaaatg ccgcttgttc ggtgttgcgg
    16261 ctgtgcagtt tatgcaccgg cttaatcttc ttcagaatat gcgagccgag cgtcagacta
    16321 tttttaatgg tatctgtttg caccagatca aacagcactt gttgcaatag gtgatccttg
    16381 tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt cgcgttcaga caggtatttc
    16441 gccattgcgc tacttgcccc acggttagag agggcaaata ccgaagcacc ggcagctttc
    16501 gggttggtaa agccatcggc atgaattgac ataaacagat ctgcgccatg tttatgggcg
    16561 atttcaacgc gatcgtaaag tgggataaac gtatcgccag aacgcgttaa acgcgcatca
    16621 atcccatgat tacgcaaaat ggaacggacg tttttagcaa tcgccagcac cacatgtttt
    16681 tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc cgccgtgacc tggatcgaga
    16741 acaacgacac gtttgccgcc agattttttg gctttcggct tgctgtgtcc gttgctggtt
    16801 tttaaaagtt cgtctttggc gatggcttgc gacattcctg acaacgtcag ggcagccaaa
    16861 ccggctttca gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa agtgctcata
    16921 cggcctgagt tgtaataata aagttccaga tgttatatcg tatcgcgtaa tccgttacga
    16981 cggtttgatg ttgagaattg ttttactttt catttcaata gttgacaact ccccattatg
    17041 ccactttttc aaccgatttt cgtcagatat tggctaattc gaccgttcac gccataatca
    17101 gccttttaac ccgcaaaaag gtaacgaata ccatggagat acgcgtattt cgccaggaag
    17161 atttcgaaga ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg tggaacgatc
    17221 cggaaatgga catcgagcgt aagatgaacc atgacgtcag tttgtttttg gtcgctgagg
    17281 taaacggtga agtggtcgga acggtgatgg gcggttatga cgggcatcgc gggtctgctt
    17341 attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat tgccaatgcg ttgcttaatc
    17401 ggctggagaa aaagctgatt gctcgtggct gcccgaaaat tcagatcaac gtgccggaag
    17461 ataacgacat ggtgctcgga atgtatgaac gcctgggata tgaacacgcc gacgtgctga
    17521 gtctgggtaa gcgtttgatt gaagatgaag agtactgagt ttcatcctgt ccattatgat
    17581 gcgcacggtc gcctgcgttt acccttgctc ttctggcttg tgctattgct tcaggcgcga
    17641 acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc agggtactgc gctgctaaat
    17701 ctgttttatc ccgatcacga taatttctgg ctggggctga ttcctggcat tccggcggtg
    17761 ctggcgtttt tgttgagcgg tcggcgggct acgtttcctc gcacctggcg tgtactctat
    17821 tttctgttgc tgttggcgca ggtggtttta ctttgctggc aaccgtggct gtggctgaac
    17881 ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg cggatattgt ggcgttaatc
    17941 tggctgctga ccaatcgacg tttacgcgct tgttttaatg aagaaaaaga ataacggcac
    18001 tttttggtga atttgcactc caagcaacgt tattgaataa ccaaaggaag tgacatgaaa
    18061 tcgctgcgtt taatgttatg cgctatgccg ttgatgctga ccggctgttc cacgatgtcg
    18121 tcagttaact ggtcggccgc taacccgtgg aactggtttg ggtcgtccac caaagtgagc
    18181 gagcagggcg ttggtgaatt aacggcgtcc acaccactgc aagaacaagc cattgccgat
    18241 gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa ccgcgaacgg caacgtggtg
    18301 cgcttttttg aagtgatgaa gggcgacaac gtggcgatgg tgattaacgg cgatcaaggc
    18361 acgatcagtc gcattgacgt gctggatagc gatattcctg ctgacaccgg tgttaaaatc
    18421 ggtacaccgt ttagcgacct ttacagcaaa gcatttggca attgccaaaa agctgatggt
    18481 gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc aacatattag ctaccagttc
    18541 agcggggaat ggagtggtcc tgaagggtta atgccttcgg acgataccct gaaaaactgg
    18601 aaagtcagta aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc cgccgcgaaa
    18661 aaacacgtaa aatagcgctc aacaatgcca cggattgcgt ggcattatta ttttcaggag
    18721 gaacaatgtc tcaggttcag agtggcattt tgccagaaca ttgccgcgcg gcgatttgga
    18781 tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa acatttgccg
    18841 acaaactggc aacttttgaa gcgaaattcc cggacgcgca tcttggtgcg gtggttgcct
    18901 ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg ggcagaagag ctgaaagatt
    18961 ttccgggcta cggtaaaggc cttgcgccga ccacccagtt cgatgtgttg atccacattc
    19021 tttctctgcg tcacgacgta aacttctctg tcgcccaggc ggcgatggaa gccttcggtg
    19081 actgcattga agtgaaagaa gagatccacg gcttccgttg ggttgaagag cgtgacctga
    19141 gcggctttgt tgacggtacg gaaaacccgg cgggtgaaga gacgcgtcgc gaagtggcgg
    19201 ttatcaaaga cggcgtggat gcgggcggca gctatgtgtt tgtgcagcgt tgggaacaca
    19261 acctgaagca gctcaaccgg atgagcgttc acgatcagga gatgatgatc gggcgcacca
    19321 aagaggccaa cgaagagatc gacggcgacg aacgtccgga aacctctcac ctcacccgcg
    19381 ttgatctgaa agaagatggc aaagggctga agattgttcg ccagagcctg ccgtacggca
    19441 ctgccagtgg cactcacggt ctgtacttct gcgcctactg cgcgcgtctg cataacattg
    19501 agcagcaact gctgagcatg tttggcgata ccgatggtaa gcgtgatgcg atgttgcgtt
    19561 tcaccaaacc ggtaaccggc ggctattatt tcgcaccgtc gctggacaag ttgatggcgc
    19621 tgtaagcgtt tctgattcat tgattaaagg ccaggcagta acattactga ctggcctttt
    19681 tattactttt gctttaacgc cgcatacacc tgatcaacta cccaaccata cgttgcaatc
    19741 ggcagctgac cgctttcgaa catattggga ttcttttgcg gatcggcaac cggcgaatgt
    19801 ggcttgttgc ttaacatcac aatcgccata tgattcaccg gatcgataac ggtcaccgtt
    19861 ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa cgtcggcgtc
    19921 atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc cttagagctg
    19981 gtggtgaaca tcttcaccgt ttccgcactg aacagctgca catcgccata gcccccgccg
    20041 ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa acctgcgtgc
    20101 ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc ccagagagtg
    20161 gaggtgcgga tattcgggaa atggatcacg ccatcgcggg tattgccgtt caattccgtg
    20221 gcggcaattt gctgcggttt aaagcctttc agcagcgggt taaataccgt atgtgtcagg
    20281 ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc ggtgaccgac
    20341 tcgacgataa atccaagcag catataatcg acatcactgt aaatatgctt gctgccgggc
    20401 tgatactcca gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc ctgggaatat
    20461 aacgcgcccg cgaccgcttt attcggatat tgtgggtccg ccgggaaacc gccgctgtga
    20521 tgcagcaggt cagaaatccg cagcgtgttt ttgcctttga tggtgtcatt tggactatcg
    20581 gcaaaccccg gaatatattt cgcaatccga tcgtcaggat gcagtttgcc ttcgctcatc
    20641 agcttttgca gggcgaagtt cgtggcgtac attttggtgt ttgaggccag gtcatacagc
    20701 gtcccggtgg tggctttgac aggctgttcc atcaacacgc tgccatcgta ctttttcgcc
    20761 gctccccagg ctttgcgata cacaatctga ttatctttaa tgatcagcag gtttacgccg
    20821 ggataaccgg cgtccacttg ctggctaatc cagcgatcca tctggttaag ccgttcgacg
    20881 ttgaaccctg ctttctctgg cgaactttct gtcagaacag ggtattttgc ggcgctgacg
    20941 ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat atcagtccag
    21001 attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc atgccaccag
    21061 aatcccgcca gcataaaccg ccattgccgg aaggatgcct tgtgcggaag tcatcagcgg
    21121 cagcgccacc agaccagacg gcccaaaggc gctgtttaag cccatcggca gaccccacca
    21181 ggctatcagg ccgataaaca aaccgcccgc tgcgccgcct aaacaggcgg taataaacgg
    21241 tttcatgcgg ggcagggtga cgccgtaaat caacggttca ccaacgccca gcagaccggg
    21301 aataatcgcc ccgcgtacct gactgcgtaa tgcactgtgc ggttgcgccc gccagtagag
    21361 tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa ataagctgtt
    21421 gaatccctgg ctgtccatta acgcgaggta aacaggaata aagccctgat gcacgccaaa
    21481 taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgaacggat tactgttcag
    21541 gtgcataaac agccatgaca taccttcgaa taaccagcca cccagcggca taatgatcag
    21601 gtacgcaagc gtagcggtga tcagcagggt gattaacgag gtcagcagca tgtcgagatc
    21661 gtccggcata aagcggcgca ccatgccttc aatgcgggcg caggcccacg cggcaatcag
    21721 cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt gaaaaccggc
    21781 gtagtaaccg gtggttgcag cggggttata accgagcaaa aagagtgcgg caataattgc
    21841 gccattgacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca gaatcaccag
    21901 gaaagtgaac aaacctttgc tgaacacctt cataaaattc agcgcgtcgg gtagcgtgcc
    21961 ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc ccagcagcag
    22021 accggcggca ataaaaccgg ggatcagcgg cgtgaagatg gtggcgaatt tggcgagaaa
    22081 ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg cggcgatttc
    22141 tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc atcgcttttg cggcacgatg
    22201 cgctttacca ggcccaaaaa cgacctgcac ctggtcgctg gtcaaaatga cgcccttcac
    22261 gccttccagc gttttgatat tgggatcaac cagtgaactg tcatgtacac ccagacgcag
    22321 gcgcgtcata cagttaccac aactggcgat atttcccggt ccgccgacac gggtaaggat
    22381 ggtgttcaga agttcactgc tgatctcttt ggccatgggt tattccttgt ctaaaacctg
    22441 acgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat cgagattttt
    22501 cagcaccatc acaatggccg ttttacagtt gcgctcgcag gcaattaacg ccgcttccgc
    22561 ttgttcggca ttacatccgg tggcgttttt aacgatattg acctggcgca catgcagttt
    22621 ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccag atttaatcat
    22681 cagcccggtg gaaagcatat tgagcaccag tttctgcgct gtgcctgctt tcattcgcga
    22741 agaaccagtg accacttcgg cacctacaat cggtgtaatg gcaaactcgg ctgtggtgga
    22801 aacggcactc cccggattac aggaaattcc cactgtgcgg cagccgagct ggcgtgcgta
    22861 ttccagtccg gcaatcacat acggcgtgcg accgctggca gcaatgccaa ccaccacatc
    22921 ctgtgccgtt aaattaatat ttttcagatc gttaacacca ccttcccggc tatcttcagc
    22981 gccttccacc gcgtgctgaa tggcatattc gcctccagca atcaaaccaa ccaccagacc
    23041 cggtttcacg ccgtaggtgg gcggacattc gctggcatcc agaatcccca gacgaccgga
    23101 tgttcccgca ccgagataaa tcagacgacc gccgccgctg acctgggcgt ggataacatc
    23161 gatcgccgcg gcgatatccg gcaggacgcg ctcaacggca agcggtacgg ttttatcttc
    23221 atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag ccgaggcggt
    23281 gttcgagcct tcagtaatca tcttttctag ttgcatcggt ggtgacctta ctatttacga
    23341 tcaaataatt gcagatgtat ggaatcatat atcccgaatt gacagactta aaggaatcat
    23401 tgagtctaca ctcgcagagt gtgaagagcg tcataagaac ttaagcggaa tttatgctgc
    23461 cagcccagga gagtggaaga tgttgtacct gacgaaaata agcaatgcgg ggagtgaatt
    23521 caccgaaaac gagcaaaaaa tcgctgactt tctgcaggcc aacgtgagtg agctgcaatc
    23581 cgtttcttca cggcagatgg cgaaacagct cggcattagc cagtcgagca tagtgaaatt
    23641 tgcccaaaaa ctcggcgcgc aaggatttac tgaattgcgt atggcgttaa taggcgaata
    23701 cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac ctgcacagtt cgattaccag
    23761 tgatgattcg ctggaagtga ttgcccgcaa attgaatcgc gaaaaagagc tggcgctgga
    23821 gcaaacctgt gcgttgttcg actacgcgcg gctgcaaaaa atcattgagg tcatcagtaa
    23881 agcaccgttt atccagataa cgggcctggg cggatcggcg ctggtggggc gcgatctgtc
    23941 gttcaaactg atgaaaattg gctatcgcgt ggcctgcgaa gccgacacgc acgtacaagc
    24001 caccgtttcc caggcgttga aaaaaggcga tgtacaaatc gctatctctt acagcggcag
    24061 taaaaaagag attgtcttat gcgcagaagc ggcacgaaag cagggggcaa cggtgattgc
    24121 cattacttct ctggctgatt ctccgctgcg gcggctggcg catttcacgc tcgataccgt
    24181 ttccggtgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc aaaattctgt
    24241 taccgattta ctgtttgttg gtcttgtgca actcaatgat gtggagtcat tgaaaatgat
    24301 tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc gactggattg
    24361 agattttcct gaattagtga gctgatccgc agcaatattt tgtttatcct gtattttcag
    24421 agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg taaactcacg
    24481 ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat tgccagaact
    24541 tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat cgaaaagctg
    24601 gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga tgtgcgtgac
    24661 ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg cattgatatc
    24721 ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag cgatgacgat
    24781 cgcgatttcc atattgacat caatattaaa ggcgtatgga acgtcacgaa ggcggtgctg
    24841 ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcggt cactggcgat
    24901 atggtggccg atccgggtga aacggcgtac gccttaacga aagcggcgat tgttggcctg
    24961 acaaaatcgc tggcggtgga gtatgcgcag tctggtattc gcgttaacgc catatgccct
    25021 ggatacgttc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc ggaagatcca
    25081 gaatcggtgc tgactgaaat ggcgaaagca atcccgatgc gtcgcctcgc cgatccgttg
    25141 gaagtcggcg aactggcggc cttcctcgca tcggatgaat ccagctattt aaccggtaca
    25201 cagaatgtga ttgatggcgg cagcacactg ccggagacgg tcagcgtcgg tatctgattc
    25261 acctctgttt cctccctgca tgtgtgggga ggatttcgtc ttattccctt ttcaacttcc
    25321 aaatcaccaa acggtatata aaaccgttac tcctttcacg tccgttataa atatgatggc
    25381 tattagaaag tcattaaatt tataagggtg cgcaatggcc gttaacttac tgaaaaagaa
    25441 ctcactcgcg ctggtcgctt ctctgctgct ggcgggccat gtacaggcaa cggaactgct
    25501 gaacagttct tatgacgtct cccgcgagct gtttgccgcc ctgaatccgc cgtttgagca
    25561 acaatgggca aaagataacg gcggcgacaa actgacgata aaacaatctc atgccgggtc
    25621 atcaaaacag gcgctggcga ttttacaggg cttaaaagcc gacgttgtca cttataacca
    25681 ggtgaccgac gtacaaatcc tgcacgataa aggcaagctg atcccggccg actggcagtc
    25741 gcgcctgccg aataatagct cgccgttcta ctccaccatg ggcttcctgg tgcgtaaggg
    25801 taacccgaag aatatccacg attggaacga cctggtgcgc tccgacgtga agctgatttt
    25861 cccgaacccg aaaacgtcgg gtaacgcgcg ttatacctat ctggcggcat ggggcgcagc
    25921 ggataaagct gacggtggtg acaaaggcaa aaccgaacag tttatgaccc agttcctgaa
    25981 aaacgttgaa gtgttcgata ctggcggtcg tggcgcgacc accacttttg ccgagcgcgg
    26041 cctgggcgat gtgctgatta gcttcgaatc ggaagtgaac aacatccgta aacagtatga
    26101 agcgcagggc tttgaagtgg tgattccgaa aaccaacatt ctggcggaat tcccggtggc
    26161 gtgggttgat aaaaacgtgc aggccaacgg tacggaaaaa gccgccaaag cctatctgaa
    26221 ctggctctat agcccgcagg cgcaaaccat catcaccgac tattactacc gcgtgaataa
    26281 cccggaggtg atggacaaac tgaaagacaa attcccgcag accgagctgt tccgcgtgga
    26341 agacaaattt ggctcctggc cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt
    26401 agacaagctg ttagcggcgg ggcgtaactg atgtttgctg tctcctccag acgcgtgctg
    26461 ccgggcttta ccttaagcct cggcaccagt ctgctgtttg tgtgcctgat tttgctgctg
    26521 ccgctctccg cgctggtgat gcaactggcc cagatgagct gggcgcagta ctgggaggtg
    26581 atcaccaacc cgcaggtggt cgcggcctac aaagtaacgc tgctgtcggc gtttgtggca
    26641 tcgattttta acggcgtttt cggtctgctg atggcgtgga tcctaacccg ctatcgcttc
    26701 ccaggccgca cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc aacggcagtc
    26761 gccggtttaa cgctagcctc gctcttttct gtaaacggtt tttacggtga atggctggcg
    26821 aagtttgata tcaaagtcac ctatacctgg ctggggattg cggtggcgat ggcctttacc
    26881 agcattccgt ttgtggtacg taccgtgcag ccggtgctgg aagagttagg cccggaatat
    26941 gaagaagcgg cggaaacgct tggtgcaacg cgctggcaga gtttctgcaa agtggtgctg
    27001 ccggagcttt ctccggcgct ggtggcgggc gtggcgctgt cgtttacccg tagtcttggt
    27061 gaatttggcg cggtgatttt tatcgccgga aatatcgcgt ggaagacgga agtgacgtcg
    27121 ctgatgattt ttgtgcgctt acaggagttt gattacccgg cagcgagcgc gattgcttcg
    27181 gtgatcctcg cggcatctct gctgctgctg ttctcaatta acactctgca aagtcgcttt
    27241 ggtcggcgtg tggtaggtca ttaatggcgg aagttaccca attgaagcgt tatgacgcgc
    27301 gcccgattaa ctggggcaaa tggtttctga ttggcatcgg gatgctggta tcggcgttca
    27361 tcctgctggt gccgatgatt tatatcttcg tacaggcgtt cagcaaaggg ctgatgccgg
    27421 ttttacagaa tctggccgat ccggacatgc tacacgccat ctggctgacg gtgatgatcg
    27481 cgctgattgc cgtaccggta aacctggtgt tcggcattct gctggcctgg ctggtgacgc
    27541 gctttaactt ccctggacgc cagttactgc tgacgctact ggacattccg tttgccgtat
    27601 cgccggtggt tgccggtctg gtgtatctgc tgttctacgg ctctaacggc ccgctgggcg
    27661 gttggctgga tgaacataat ctgcaaatta tgttctcctg gccgggaatg gtgctggtca
    27721 ccatcttcgt gacgtgtccg tttgtggtgc gcgaactggt gccggtgatg ttaagccagg
    27781 gcagtcagga agacgaggcg gcgattttgc ttggcgcgtc tggctggcag atgttccgtc
    27841 gtgtcacatt gccgaacatc cgctgggcgc tgctttatgg cgtagtgctg accaacgccc
    27901 gcgcgattgg cgagtttggc gcggtgtcgg tggtttccgg ctcgattcgc ggcgaaacac
    27961 tttcgctgcc gttacagatt gaattgctgg agcaggacta caacaccgtc ggctccttta
    28021 ccgctgcggc actgttaacg ctgatggcga ttatcaccct gtttttaaaa agtatgttgc
    28081 agtggcgcct ggagaatcag gaaaaacgcg cacagcagga ggaacatcat gagcattgag
    28141 attgccaata ttaagaagtc gtttggtcgc acccaggtgc tgaacgatat ctcactggat
    28201 attccttcag gtcagatggt cgcgttgctg gggccgtccg gttccgggaa aaccacgctg
    28261 ctgcgcatta tcgccgggct ggagcatcaa accagcgggc atattcgctt ccacggcacc
    28321 gacgtcagcc gcctgcacgc gcgcgatcgc aaggtcggtt tcgtgttcca gcattacgcg
    28381 ctgttccgcc atatgacggt attcgacaat atcgcttttg gcctgacggt gctgccgcgt
    28441 cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga caaaattgct ggaaatggtc
    28501 cagcttgcgc atctggcgga tcgttatccg gcgcagcttt ccggcggcca gaaacagcgt
    28561 gtggcgctgg cgcgcgcgct ggctgtagaa ccgcaaattc tgctgcttga tgaaccgttt
    28621 ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca actgcatgaa
    28681 gagctgaaat tcaccagcgt gtttgtgacc cacgaccagg aagaggcgac cgaagtagcc
    28741 gatcgtgtag tggtgatgag tcaggggaat attgaacagg ctgatgcgcc gaatcaggta
    28801 tggcgcgaac cagcgacccg ttttgtactc gaatttatgg gcgaagtgaa ccgcctgcag
    28861 ggaaccattc gcggcgggca gttccatgtt ggcgcacatc gctggccgct gggctacaca
    28921 cctgcttatc aggggccggt ggatctcttc ctgcgcccgt gggaagtgga tatcagccgc
    28981 cgtaccagtc tcgattcgcc gctgccggta caggtactgg aagccagccc gaaaggtcac
    29041 tacacccaat tagtggtgca gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg
    29101 catggcgacg atgccccgca gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg
    29161 ctgtataacg gcgacgagcg tatcgaaacc cgcgatgagg aacttgctct cgcacaaagc
    29221 gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt
    29281 aatggcaagg tttgtaacct gtagacctga taagacgcgc aagcgtcgca tcaggcaaca
    29341 ccacgtatgg atagagatcg tgagtacatt agaacaaaca ataggcaata cgcctctggt
    29401 gaagttgcag cgaatggggc cggataacgg cagtgaagtg tggttaaaac tggaaggcaa
    29461 taacccggca ggttcggtga aagatcgtgc ggcactttcg atgatcgtcg aggcggaaaa
    29521 gcgcggggaa attaaaccgg gtgatgtctt aatcgaagcc accagtggta acaccggcat
    29581 tgcgctggca atgattgccg cgctgaaagg ctatcgcatg aaattgctga tgcccgacaa
    29641 catgagccag gaacgccgtg cggcgatgcg tgcttatggt gcggaactga ttcttgtcac
    29701 caaagagcag ggcatggaag gtgcgcgcga tctggcgctg gagatggcga atcgtggcga
    29761 aggaaagctg ctcgatcagt ttaataatcc cgataaccct tacgcccatt acaccactac
    29821 cgggccggaa atctggcagc aaaccggcgg gcgcattacc cattttgtct ccagcatggg
    29881 aacaaccggc actatcaccg gcgtttcacg ctttatgcgc gaacaatcca aaccagtaac
    29941 gattgttggc ctgcagccgg aagaaggcag cagtattccc ggcattcgcc gctggcctgc
    30001 ggaatatctg ccggggattt tcaacgcttc tctggtggat gaggtgctgg atattcatca
    30061 gcgcgatgca gaaaacacca tgcgcgaact ggcagtgcgg gaaggaatat tctgtggcgt
    30121 cagctccggc ggcgcggttg ccggagcact gcgggtggca aaagctaacc ctggtgcggt
    30181 ggtggtagcg atcatctgcg atcgtggcga tcgctacctt tccaccgggg tgtttgggga
    30241 agagcatttt agccaggggg cggggattta agattattgc gcaggcaccg ttttatccgt
    30301 ctgcgcaatt agcgcatgta aaaaaggctg tgcgttctca tcgctcttac cggggatctt
    30361 cacaatatac ggcttcccgg taatcgatga cgacgaagca accttatcaa taaactgctc
    30421 ggcagtgtca atgcggttac gggtattgcc cagcttcaga cgcagatgag aaaccgcttc
    30481 atcgcaggta tgttcatcac cgttgcgtac aaatatcaaa tcctttttct gtgctaatcc
    30541 ctccagcatg gcgttgatgc gggcttcttc atgggcagtt aacttcgcgt aagcggggag
    30601 tgtcattagt agtgtaatga ccagacaaat gattttcttc attgaattac cttatccttg
    30661 ttttgtgata gccgatttga ctgccagaat caggtaaagt ttcggttgga atacgatgag
    30721 gatcatcggc aaggagaaaa aattaaatga gtagtttgtt gttgtttaac gataagagca
    30781 gggcgctgca ggcggatatc gtcgccgtgc agtcgcaggt ggtttacggc agcgtgggca
    30841 acagcattgc cgtgcctgct atcaaacaga acggtctgaa tgtttttgcc gtgccgacgg
    30901 tattgctgag caatacgccg cattatgaca ctttctacgg tggtgcgatt ccggatgaat
    30961 ggtttagcgg ttatttgcgt gcgcttcagg agcgtgatgc gctgcgccag ctgcgcgctg
    31021 taaccacggg ctatatggga acggcatcgc aaatcaaaat ccttgccgag tggctgactg
    31081 cgctacgcaa agaccatcct gacctattga tcatggtcga tccggtgatt ggcgatattg
    31141 atagcggaat ttatgtcaaa cctgaccttc ccgaggcgta tcgacaatat ttactgccgc
    31201 tggcgcaggg aattaccccc aatatctttg agctggaaat cctgaccggt aaaaattgcc
    31261 gtgatctcga cagtgccatt gctgccgcaa aaagtctgct ttcagacaca ttaaaatggg
    31321 tggtgattac cagcgcctcc ggtaatgaag aaaatcagga gatgcaggtg gtggtggtca
    31381 gtgccgacag cgtgaatgtc atttcccatt cacgggtaaa aaccgacctg aaagggactg
    31441 gcgacctgtt ttgtgctcag cttatcagtg gtttgctgaa agggaaggcg ttaaacgatg
    31501 cggtgcaccg tgcggggttg cgcgtactgg aagtgatgcg ctacacccag cagcatgaga
    31561 gcgatgaatt gattttgccg ccgctggcgg aagcataaaa aatggcgccg atgggcgcca
    31621 tttttcactg cggcaagaat tacttcttga tgcggataac cggggtttca cccacggtta
    31681 cgctaccgga cagtttgatc agttctttga tttcgtccat gttggagata acaaccggag
    31741 tcagggtaga cttggctttc tcttccagta gcggcagatc aaattcaatg acagtatcgc
    31801 caactttcac gcgctgacct tcttcagcaa tacgcttgaa gccttcgcct ttcagttcaa
    31861 cggtgtcgat accgaagtgg acgaacagtt caacgccgct atcagattcg atagagaacg
    31921 cgtgattggt ttcaaagatt ttaccaatgg taccgtctac aggcgcgacc attttgttac
    31981 ccgttggttt gatagcaata ccatcaccaa cgattttttc cgcaaaaacg acatccggca
    32041 cgtcttcgat attgacgatc tcgccagaga gcggagcaat gatctcaata gttccggtat
    32101 ccttcttgtc gtcggaaacc agagatttca gtttatcgaa caaacccatg atcttctcct
    32161 aagcagtaaa ttgggccgca tctcgtggat tagcagattg ttttttcttc aatgaacttg
    32221 ttaaccagcg tcattaactc gtccgttgtc ggttgagcaa gagcctgctc tgctaacacc
    32281 ttcgcatctt cgaagttcgt gttacggata atcttcttaa tgcgcgggat agaaatggcg
    32341 ctcatagaga attcgtccag acccatcccc agcaacagaa gtgtagcacg ttcatcgcca
    32401 gcaagctcac cacacatgcc agtccatttg ccttcagcat gagaagcatc aataacttgc
    32461 ttgatcaagt tcagcacgga cggtgacatt ggttggtaaa ggtgtgaaat catatcatta
    32521 ccacggtcaa ctgccagagt gtactgcgtt aaatcattgg tgccgatact aaagaaatca
    32581 acttctttgg ctaaatgacg tgcaattgtt gcggcagccg gtgtttccac cattacgccg
    32641 atttcaattg actcgtcaaa cgctttacct tcgtcgcgca gttcctgttt gtagatttcg
    32701 atctctttgc gcagtgcacg cacttcttca acagagatga tcatcgggaa cataatgcgc
    32761 aatttaccga aagccgaggc acgcaggata gcgcggagct gatcgcgcag gatctcttta
    32821 cgatccatcg cgatacggat agcgcgccag ccgaggaacg ggttctcttc tttcgggaag
    32881 ttcatgtatg gcagctcttt gtcgccgccg atgtccatgg tacgaacgat aaccgcctgc
    32941 gagccacacg cttcagccac tgctttgtaa gcagcaaact gttcttcttc agtgggcagc
    33001 gcgtcgcggt ccatgaacag gaactcagta cgatacagac caacgccttc agcgccgtta
    33061 cgctctgcac cttcaacgtc acgaaccgta ccaatgttag cgcacacttc tacctggtga
    33121 ccgtccagcg taatagctgg cagatctttc agtttagcaa gctctgcttt ttcagaagcc
    33181 acttgctcct gaacagcgcg cattttatca ataacttcgt tggttggatt gacgtaaacc
    33241 tgattattta cggcatccag aatcagatag tcgtcatttt tcacctgaga ggtgacgcta
    33301 ccggtaccca cgatagcagg cagttccaga gaacgcgcca tgatagaggt gtgggaagta
    33361 cggccacccg cgtcggtgat gaaacccagc accttcttca ggttcagctg tgcggtttcg
    33421 gacggcgtca ggtcagcggc aaccagaatg acttcatcct gaatggcgct caggtcgata
    33481 atcttcaggc ccaggatgtt gcgcagcagg cgcttaccga tatcacgtac gtcagccgca
    33541 cgttctttca ggtattcatc atccagctct tccagggcag aagcctgacc ttcgataact
    33601 tcatgagcag ctgcgtcagc tgtcatgtgc ttatctttaa tcagggctat gatttcctgc
    33661 tccagctcct catcttcgag cagcataata tgcccttcaa agatggcttc tttttcttca
    33721 ccgaacgttt caccagcttt cgttttgatc gtttccagct gggctgatgc cttggcacga
    33781 ccgctcagaa aacgttcaac ttcctgatca acctggtcgg cagaaatttt tttccggtca
    33841 atgacaattt cgtcttcttt cagaagcaga gctttaccga aagcgatacc cggggatgct
    33901 aaaatgcctg aaatcataac cctaccttac ttgtgactga tttttaaaag aacccgggaa
    33961 attactcgag ttccgccatc agtttaacca gatgttcaac cgctttctgc tcgtcttcgc
    34021 cttctgcgga gatagtcaca acggtacctt gagtcaggcc cagagtctgc agtttaaaca
    34081 ggcttttcgc gctggcgctt ttgccgttgg aagtcacagt aatttcagaa gtgaagccct
    34141 tagcttcttt tacaaactgg gcagcagggc gggtgtgcag accgttcgga gcggtaatgg
    34201 taacttcttg ctggaacatt gtatttcccc aacttatagg tttagtgttg tggaactaaa
    34261 gtctagcctg gcggttcgac tttagcctgt attgttagcg ccagttttta acagacgcga
    34321 cgcacgaaga gcgcaataca gtccattgct ggcgggtgtt gaccgctgac gtttcgtacg
    34381 tcattaaaca ttatgccgct aaaggaagaa ttgaaccaaa tcataaaatc gattcagctt
    34441 tggcttttcc tgtaacgatt aatttcgcgc atcaaaataa ttagtctggt taaataccag
    34501 ttccgacagg ctgaatcaat gccaggaggg gcgaaagttt gaagcaggcc acaaaaaagc
    34561 acctaaaaag gtgctttttt acgcattttt aacaagctgg cattactgtt gcagttcttt
    34621 ctcagtgaag agatcggcaa acaatgcggt gcttaaataa cgctcacccg atgacggtag
    34681 aataaccaca atattcttgt tggtaaagct ttcatcttct tgtagtttca acgccgcagc
    34741 aacagctgct ccagaagaga tacctgcaag aataccttct tcttccatca gacgacgtgc
    34801 ggtagaaatc gcttcttcat tggtgatgcc aatgacttta tcgaccagct tgagatcgag
    34861 gttagctggg ataaaaccag cgccaatacc ctgaatttta tgcgggccag gtttaatctc
    34921 ttcaccagcc agtgcctggg cgataactgg agaatcggtt ggctcaacgg cgacagagat
    34981 aagatcggtc ttgcctttgg tgcctttaat gtagcggctg acgccagtca gcgtaccgcc
    35041 agtcccaacg ccagcaataa atacatcaac ctgaccgtcg gtatcttccc agatctccgg
    35101 accggtggtt ttttcgtgaa tttcagggtt tgccggattg ctgaattgtt gcagcagcag
    35161 gtatttctct ggattgctgg cgacaatttc ttctgctttt tggattgcgc ctttcatgcc
    35221 tttagcacct tccgtcagca ccaggtttgc acctaacgct ttcagcagct tgcggcgttc
    35281 aatactcatg gtttctggca tggtcagggt gagtttgtaa ccgcgagcgg cagctacata
    35341 ggccagtgca atcccggtat taccgctggt tggttcaacc agttcaacgc ctggtttcag
    35401 cacgccgcgc ttttcggcat cccaaatcat gttggcaccg atacggcact taacgctgaa
    35461 gctggggtta cgagattcca ccttcgccag aatgcgtccg ttaccgatgc gattcaggcg
    35521 aaccagcggc gtgtgaccga tagtcagcga gttatcttca aaaatcttac tcatggcctg
    35581 tccttaactg tatgaaattg ggatacaaca ggtagcatac ccgctcagag aatatgcgga
    35641 agtaaggatt tagcatatct atatacagaa gggaaataat gacatgcaag atggaataag
    35701 gggcggcata agccacccct gtttcacaca aacggtttac aaaataaccg gtagattgtt
    35761 accgccacat cgcgtgttta tcgcgatagc aatcgaccca catcgccgtc gcgccacaaa
    35821 cggcaacggg catgatgaac agattaagca gcgggatcat cgtaaacagg ctggttaaag
    35881 caccaaactg catattggtg attttgcgtg tgcgcagggc ggtgcgcatc tctttaaacg
    35941 gcactttgtg gttatcgaag gggtaatcgc aatactggat ggctaacatc caggcgctaa
    36001 acaggaacca cagtaccggc gcgacggttt gcccaatacc agggattaag taaagaatta
    36061 gcaatacaat tgcgcgcggc agataccagg caaatttttg ccattcgcgt ttcatgattc
    36121 gcggcacatc tttcatgata ccgaaaatcc cagtatctgg cggtgtagcg ccagtcaatc
    36181 gtgcttccag ttgttcagcc aataaaccgt taaacggagc ggcaatccag ttagcaatcg
    36241 tggagaagaa atagccaaac actaacagca cagagatgac agccagaggc cacaacagat
    36301 aactcagcca ttgtagccag tccggaacgt aactcatgag agtcgggatc cagacatcga
    36361 gctgtgtaaa gagccaccag aatgcgcccc ccatcaacaa aatattgacc agcagcggta
    36421 aaataacgaa acgccgaatc ccaggttgcg agacgagctt ccagccttgc gcaaaatagt
    36481 aaaaaccgct gcgtggggca gatgtgaatg atgaaaccat aatcaggatg agctcctttt
    36541 gaccaatccc aggaaaattc tgcgtatttt accgggtaat tgcgcaatgg acagttagga
    36601 tatgttcgaa aaaacagcaa aaagcacgat ttcatctatc tttgtgctgt gaaagttaat
    36661 agtgcacttg cacttgaggt aatcggcaaa tactcttagt gagtaaatgt ttgccgtggt
    36721 ggcaaggtgt tagaacaaca gagaatataa tgatgcagga tttgcgtctg atattaatca
    36781 ttgttggcgc gatcgccata atcgctttac tggtacatgg tttctggacc agccgtaaag
    36841 aacgatcttc tatgttccgc gatcggccat taaaacgaat gaagtcaaaa cgtgacgacg
    36901 attcttatga cgaggatgtc gaagatgatg agggcgttgg tgaggttcgt gttcaccgcg
    36961 tgaatcatgc cccggctaac gctcaggagc atgaggctgc tcgtccgtcg ccgcaacacc
    37021 agtaccaacc gccttatgcg tctgcgcagc cgcgtcaacc ggtccagcag ccgcctgaag
    37081 cgcaggtacc gccgcaacat gctccgcgtc cagcgcagcc ggtgcagcag cctgcctatc
    37141 agccgcagcc tgaacagccg ttgcagcagc cagtttcgcc acaggtcgcg ccagcgccgc
    37201 agcctgtgca ttcagcaccg caaccggcac aacaggcttt ccagcctgca gaacccgtag
    37261 cggcaccaca gcctgagcct gtagcggaac cggctccagt tatggataaa ccgaagcgca
    37321 aagaagcggt gattatcatg aacgtcgcgg cgcatcacgg tagcgagcta aacggtgaac
    37381 tgcttcttaa cagcattcaa caagcgggct tcatttttgg cgatatgaat atttaccatc
    37441 gtcatcttag cccggatggc agcggcccgg cgttattcag cctggcgaat atggtgaaac
    37501 cgggaacctt tgatcctgaa atgaaggatt tcactactcc gggtgtcacc atctttatgc
    37561 aggtaccgtc ttacggtgac gagctgcaga acttcaagct gatgctgcaa tctgcgcagc
    37621 atattgccga tgaagtgggc ggtgtcgtgc ttgacgatca gcgccgtatg atgactccgc
    37681 agaaattgcg cgagtaccag gacatcatcc gcgaagtcaa agacgccaac gcctgataca
    37741 cttaaggcaa attaactcct cttcgaaccc ccgcttgtcg ggggttttta gcattgatgg
    37801 tgcgatatgg aatcaatcga acaacaactg acagaactgc gaacgacgct tcgccatcat
    37861 gaatatcttt atcatgtgat ggatgcgccg gaaattcccg acgctgaata cgacaggctg
    37921 atgcgcgaac tgcgcgagct ggaaaccaaa catccagaac tgattacgcc tgattcgcct
    37981 acccaacgtg taggcgctgc gccgctggcg gctttcagcc agatacgcca tgaagtacca
    38041 atgctgtcac tggataacgt ttttgatgaa gaaagctttc ttgctttcaa caaacgtgtg
    38101 caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct gtgagctgaa gctggatggc
    38161 cttgccgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat
    38221 ggcaccaccg gggaagatat cacgtctaat gtgcgtacta ttcgcgctat tccgctgaag
    38281 ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag
    38341 gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt ttttgctaac
    38401 ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac agcgaagcga
    38461 ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct gccggatact
    38521 catcttggcc gtttactgca atttaaaaag tgggggttgc cggtcagcga tcgggtaacg
    38581 ctttgtgaat cggcggaaga agtgctggcg ttctatcaca aagtggaaga agaccgcccg
    38641 acgctgggct ttgatatcga cggcgtggtg attaaggtca actcactgga acagcaggag
    38701 cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag cgtttaaatt cccggcgcag
    38761 gagcagatga cctttgtgcg tgacgtcgag tttcaggttg ggcgtactgg cgcgattacg
    38821 cctgttgcgc gtctggaacc tgtccatgtt gctggcgtgc tggtgagtaa cgcaacctta
    38881 cacaatgcgg atgaaatcga acgtcttggt ttacgcattg gcgataaagt ggtgattcgc
    38941 cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc tttctgaacg cccggaagat
    39001 acccgtgagg ttgtattccc gacgcattgt ccggtatgtg gttctgacgt tgagcgtgtg
    39061 gaaggtgaag cggttgcccg ctgtaccggt ggcctgattt gcggagcgca gcgtaaagag
    39121 tcgctgaaac actttgtttc ccgccgtgcg atggatgttg acggaatggg cgacaaaatc
    39181 atcgatcagc tggttgaaaa agaatatgtc catactccgg cagatctgtt caaactcacc
    39241 gcaggcaaac tgaccggact ggagcgtatg gggccaaaat cggcacaaaa cgtggttaac
    39301 gcgctagaaa aagcgaaaga aaccaccttt gctcgcttcc tctatgcact tggcatccgt
    39361 gaagtcggcg aggccaccgc agcaggtctg gcggcatatt tcggcacgct ggaagcgctg
    39421 gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg ttggcattgt cgttgcatcc
    39481 cacgttcaca acttctttgc cgaagaaagc aaccgcaatg tcatcagcga gctgttggcg
    39541 gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg aagagattga cagcccgttt
    39601 gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga tgtcgcgtga tgacgctaaa
    39661 gctcgactgg ttgaactggg cgcgaaagtc gcgggcagcg tgtcgaagaa aaccgatctg
    39721 gtgattgccg gagaagcggc aggttctaaa ctggcgaaag cgcaggaact gggcattgaa
    39781 gtcatcgacg aagcggaaat gctgcgtttg ctgggtagct gagatggaaa aagagcagct
    39841 gattgaaata gccaatacga taatgccgtt tggcaaatat aaagggcgtc gcttaatcga
    39901 cctgccggag gagtatctgc tgtggtttgc ccgcaaagat gaattcccgg cagggaagct
    39961 cggtgagcta atgcaaatca cgctgctgat taaaaccgag gggctgacgc aactggtcca
    40021 gcccctgaag cgtccgcttt aagctttatc ggcgctgctt tcctgctgtg cctgtaactg
    40081 ttcggtctgg cgtttgtatc gacgcgccag caccgcacag accatcaatt ggatctgatg
    40141 gaaaatcatt aggggcagca ccatcatacc gatcaccgat gtggggaaca gaatgtttgc
    40201 catcgggatg ccatttgcca gactcttttt cgaaccacaa aagacgatag taatttcatc
    40261 tgccttattg aagcccagtc ggcgtgccat aaagacgtta actacaatca cgatagccag
    40321 aagaacgcag ctgaccacca cgataaacag caatgatccc cagccaactt tatgccagat
    40381 accattaacg acggcttcgc tgaacgctgt ataaaccacc aacagaatgg acgtctggtc
    40441 agttttcgca atccattttt tattgcgcga cacccagtca ccaatccacg gccgggaaag
    40501 atgccccaac acaaacggca gcagcagttg cagcataatt ttaccgacct gctcaaggct
    40561 gccccctgca ccgtgaacat tcatcaccag accaaccagc aatggtgaaa ggaaaatccc
    40621 cagcaggctg gatgctgacg cagaacaaac cgccgccgcg acgttaccgc ccgccattga
    40681 cgtgaaggcg attgcagact gcacggtagc cgggagaatg cacaagtaga gaaaaccgga
    40741 gtagagcatc gggtcgacat ttaccggttt ccaccaggca aacagtacac ccagaatcgg
    40801 aaacagcacg aaggtgctgc aaattaccca caaatgcagt cgccagtgac caccgccagc
    40861 aataatcgcc tcacgcgaca acttcgcgcc gtgcataaag aacagcaggg caatcgctgc
    40921 ggtggtcaga ttttcaaaga aggggacgaa atcgcttctg gccggaaaga aagaggccag
    40981 caacaccacc gtgatcaggg ttaaggtgaa aggatcgagg atacgaaaaa gtttcataaa
    41041 gactccagat gatcgatggt tctattgtgc ttcttccagt ttgagaaata aaattgattt
    41101 attgcatcca ttcatgaaga aaacagatga attattcttt aaaacaatta aaggttttcg
    41161 tcacagtagc gcaggagaaa agttttagtc gtgcaggaga gcgtatcggc ctgagccagt
    41221 cggcagtgag tcacagtgtg aaggaactgg aaaatcatac tggtgttcgc ctgctggaca
    41281 gaaccacgcg tgaagtggtg cttacagatg cagggcagca gctggctttg cgtcttgagc
    41341 gactgctgga tgaactgaac agcacgttgc gcgataccgg gcgtatgggg caacaactga
    41401 gcggaaaagt tcgggtcgct gccagccaga ccatttccgc gcatcttatt ccgcaatgca
    41461 ttgctgaaag ccatcgccgc tatccagata ttcagtttgt cctgcacgat cgtccgcagc
    41521 agtgggtgat ggaaagtatt cgccagggag atgtcgactt tggcatcgtc atcgatccag
    41581 gcccagtggg cgatctgcaa tgtgaagcga ttctttccga gcctttcttt ctgctttgcc
    41641 atcgcgatag cgctttggcc gtcgaagatt atgtaccctg gcaggcatta cagggagcga
    41701 aattggtgtt gcaggattac gcgtcaggca gccgaccgct gattgacgca gcgctggcgc
    41761 gcaatggtat tcaggcgaat attgtgcagg agattggaca tccggcgacg ctgttcccga
    41821 tggtagccgc aggcatcggc atcagtattc ttcccgcact ggcgctacca ttgcctgaag
    41881 gtagcccact ggtggtaaag cgcattacgc cggttgttga acgccagttg atgctggtgc
    41941 gcaggaagaa ccgttcactt tccaccgccg ctgaagcgtt atgggacgtg gtacgagacc
    42001 agggcaatgc gctaatggct gggcgggaag gggatccgtt gtatcagata tagagtgaat
    42061 tcatactgcc gggagccccg gcagtaatca atcagaagtt aaatttgatg gagtagatta
    42121 tcgcatcctg gtaaaaatct tcgcccagtt tgtcatccgc atatcgatac tgaatccccg
    42181 tagtaatgtg tgatgttgca ttccaccaca gtgcaactgc gccattcagc ccttctttac
    42241 cgccattacc cgctgcatag gtagcgtcac ggtcaaactc gtactcgttc cagttggtaa
    42301 gagtgaattt ctcgctgccc agcataaagt tgtaacctgc aacccagccg gcgacatagc
    42361 cgttgtcgcc agtgtaatag gtttgatctg tataacgctt agcaaagaac ggtttgaacc
    42421 accaaccgct gccggtgaaa ttgtagccga taccgtacag gaacatatcg tcgtggaaat
    42481 tcacgcgatt cgcagaaccg taggtgccat acgcatgcaa gtagagatta aatccggtgt
    42541 cacccaggta aatacggttg gtatttttaa aggtataacg ctgctcgcta cctggtttat
    42601 tatggcgacc gttatagaag ttttcccagt cgaagaaccc gtacatttct ccccaactaa
    42661 agttagcgcc accttcaaat tcaagataac caaaatcatc tttgtgtgat ttggtcgacg
    42721 atttttcggt agtccggctg gtccagtcca gataatgcac gccgatatct gcaaaaccgc
    42781 ctttaaattc tgcatgagat acaacaggta tcgctaatat agaggaaagt gtcagagtta
    42841 aaagatgttt tttcatagca taattcccta tgccgatcgt tattagtaga ttgcttaata
    42901 aagtggtttg ctaaaaacga catattgtta atgcaaagga attaatatcg ccaatacagt
    42961 ttttaccgtg gattaataga gaaaagatat aaatccatcg atatcgaatc gctattgaat
    43021 gcgtgagaaa agtgtattgg taagagccgg gacaacctcg ctataaaaag gcgttagatt
    43081 ccaccctaca gaaaaaggat atgtatgtct caggttcaat tttctcataa cccgctgttt
    43141 tgcatagata ttatcaagac ttataaacct gatttcacgc cacgagtggc ctttatttta
    43201 ggttccgggc tcggcgcgct ggccgatcag attgagaacg ctgtcgcaat ttcctacgaa
    43261 aagctgccgg gcttcccggt aagtacggta catggtcatg cgggggagtt agtgctgggc
    43321 catctccagg gcgtaccggt ggtatgtatg aaaggtcgcg gacatttcta cgaaggtcgt
    43381 ggaatgacca ttatgactga cgcaattcgt acctttaagc tgctgggctg cgagctactg
    43441 ttctgcacca atgcggcagg ctcgctgcgt ccggaagtgg gggcaggcag cctggtcgca
    43501 ttgaaagatc atatcaacac catgcccggt acgccgatgg tgggtcttaa cgatgatcgt
    43561 tttggagagc gcttcttctc actggcaaat gcctacgatg cggaataccg cgcactgtta
    43621 caaaaagtgg cgaaagaaga gggtttccct ctgacggagg gcgtgttcgt ctcgtatccg
    43681 gggccgaatt tcgagactgc ggcggaaatt cgcatgatgc aaattattgg tggggatgtt
    43741 gttggtatgt ctgtggtgcc tgaggttatt tcagctcgcc attgcgacct taaagtcgtt
    43801 gcggtctctg cgattaccaa tatggcggaa ggtttaagcg atgtgaaatt gtcacatgcg
    43861 caaacgctgg cggctgcgga gctctctaag cagaacttca tcaaccttat ttgtggcttt
    43921 ctgcgcaaaa ttgcctgaga aaaacaataa gaaacggcct cgcgggtgag gccaaatgat
    43981 gcgacaagga aacgattatg agcatcgcga tgcgcttaaa ggtaatgtcc tttttgcaat
    44041 attttatctg ggggagctgg ctggttaccc tcggctctta catgattaat acccttcatt
    44101 tcaccggcgc taatgttggc atggtttaca gttccaaagg gatcgccgcg attattatgc
    44161 ctggtataat ggggatcatc gcagacaaat ggctgcgcgc agaacgtgca tacatgctgt
    44221 gtcacctggt gtgtgcgggc gtactttttt atgcggcatc cgtaactgat ccggatatga
    44281 tgttttgggt gatgttagtc aatgcgatgg cgtttatgcc gactattgcg ttatcgaaca
    44341 gcgtctctta ttcctgtctt gcccaggcag ggcttgaccc ggtgaccgct ttcccgccca
    44401 ttcgcgtttt tggtacggtg gggttcattg tcgcgatgtg ggcagtaagc ctgctgcatc
    44461 tggaattgag tagtctgcag ctgtatatcg cgtccggtgc gtcattgctg ctgtcggctt
    44521 atgcgctgac tttgccgaag attccggttg cggagaaaaa agcgaccaca tcgcttgcca
    44581 gcaagctggg tctggatgcc ttcgtgctgt ttaaaaatcc acgcatggcc atctttttcc
    44641 tctttgccat gatgctgggt gcggtactgc aaattaccaa cgtttttggt aatccgttcc
    44701 tacatgattt cgcccgtaac ccggagtttg ctgacagttt tgtggtgaaa tatccctcca
    44761 ttttactgtc agtttcacag atggcagaag tgggctttat actgactatc ccattctttt
    44821 taaagcgatt tggcattaaa accgtcatgc tgatgagtat ggtggcctgg acgctgcgct
    44881 ttggcttctt cgcctatggc gatccgtcaa caaccggatt tattttgctg ctgctgtcga
    44941 tgattgttta tggctgtgca ttcgatttct tcaatatttc tggttcggta tttgtcgaac
    45001 aggaagttga ttccagcatt cgtgccagcg cgcaggggct ctttatgacc atggtaaatg
    45061 gtgtcggcgc atgggttggc tcgattctga gtggcatggc agtagattac ttttcggtgg
    45121 atggcgtaaa agactggcaa actatctggc tggtgtttgc aggatatgct ctttttctcg
    45181 cagtgatatt tttctttggg tttaaatata atcatgaccc tgaaaagata aagcatcgag
    45241 cggtggctca ttaaaaggat tcgcagctct gctcttcaga gctgctttta tgataaaggt
    45301 taattagtga aagatatttt attttcttcc ggcgttggtt ttggcattgg tgctttgttt
    45361 acaatcgtgc gcttgcctat tcctgtacca aatgtattac caggtatatt atccattgtg
    45421 tttatgtatg tcggatatct ggtggtgaaa tactttatgc catgataatt taatacgatg
    45481 tatttattat atggagcact taattatgga acgcgtatac agaacagatc ttaagttgct
    45541 ccgttatttt cttgccgtag cggaagagtt gcattttggc cgcgcagcag cgcgtttaaa
    45601 tatgtctcag cctccgctca gtattcatat taaagagctg gaaaatcaac tcggcacgca
    45661 gctttttatt cgccattcgc gcagcgtcgt actgacacac gcgggcaaaa tcttgatgga
    45721 agaatcgcgt cgattgctgg tgaatgctaa taatgtattg gctcgggttg aacaaatagg
    45781 tcggggagaa gcagggcgga ttgaactcgg cgtagtggga acggctatgt ggggacggat
    45841 gcgcccggtt atgcggcgat tcctcaggga aaatcctaac gttgaagttc tttttcgcga
    45901 aaagatgccc gcgatgcaaa tggccctgct ggaacgacgt gaactcgatg ccgggatctg
    45961 gcgaatggcg acagaaccac cgacaggttt taccagctta cggttgcatg aatcggcgtt
    46021 tctggtggcg atgcctgaag agcatcatct ctcatcattt tccaccgttc cgctggaagc
    46081 gctacgtgac gagtattttg ttacaatgcc gcccgtgtac actgactggg attttttgca
    46141 gcgagtttgc cagcaggtgg gattttcacc ggttgttatt cgcgaagtta atgaaccgca
    46201 aacggtactc gccatggtca gtatgggtat tggtatcaca ttgatagcgg acagctacgc
    46261 acaaatgaac tggccaggtg tcattttccg tccgctcaaa cagcgcatcc ctgcagattt
    46321 atatattgtt tatgaaacac agcaggtgac gcccgcgatg gttaagctgt tggctgcatt
    46381 gacacagtag gtgaggagac aggaattgcc ggaaatagtc gtctgctaag tgaagcgcat
    46441 caggcaattt cgcaaatcgc agatagcaaa aaagcgcctt tagggcgctt ttttacattg
    46501 gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac
    46561 tgagctaacg acccgaagtg gtgggtgatg acgggatcga accgccgacc ccctccttgt
    46621 aagggaggtg ctctcccagc tgagctaatc acccacttcg gtacttcaca tttttacaag
    46681 aaatttagct ggcgagaaag tggtgggtga tgacgggatc gaaccgccga ccccctcctt
    46741 gtaagggagg tgctctccca gctgagctaa tcacccactt ctcaatttct tgctacacgg
    46801 cggagactac ataaagtagt tggtgggtga tgacgggatc gaaccgccga ccccctcctt
    46861 gtaagggagg tgctctccca gctgagctaa tcacccccgc tgtgtggagt cgcattatag
    46921 ggagagttca aaatgagtca acgcattttc taaagaaatt gttcgttcgt cgtaaattta
    46981 agcaagatga tcgcaaaaca gaccgtgttg cgcaatttgt caacgaaaac aataatgcgt
    47041 aaggtagaaa cccgaactac attgaggaat caggcgggag tgatagaata tcgcccactc
    47101 aatttttcca ggatttgccg gttgtcggca tctttctaaa cgtaaggcca tttcatgaaa
    47161 atcaaaactc gcttcgcgcc aagcccaaca ggctatctgc acgttggcgg cgcgcgtact
    47221 gctctttact cctggctttt tgcacgtaac cacggcggtg agttcgtgct gcgtattgaa
    47281 gacaccgatc ttgagcgttc cacgccggaa gctatcgaag ccattatgga tggcatgaac
    47341 tggctgagcc tggagtggga tgaaggtccg tactaccaga ccaaacgttt tgatcgctac
    47401 aatgcggtga tcgatcagat gctggaagag ggcactgctt ataaatgcta ttgctctaaa
    47461 gagcgcctgg aagcgctgcg cgaagagcaa atggcgaaag gtgagaagcc gcgttatgac
    47521 ggtcgctgcc gccacagcca tgagcatcat gctgatgatg aaccgtgtgt cgtgcgtttt
    47581 gctaatccgc aggaaggttc tgttgttttt gacgatcaga tccgtggtcc gatcgagttc
    47641 agcaaccagg agctggatga tctgattatc cgccgtaccg atggttcccc aacctataac
    47701 ttctgtgtgg ttgtcgatga ctgggatatg gaaatcaccc acgttatccg tggcgaagac
    47761 catatcaaca acacgccgcg ccagatcaac attcttaagg ccctgaaagc gccggtgccg
    47821 gtttacgcgc acgtttctat gatcaatggc gatgacggta aaaaactgtc caaacgtcac
    47881 ggggcagtca gcgtaatgca gtatcgtgat gacggttatt tgccagaagc actgctgaac
    47941 tatctggtgc gtctgggctg gtcccacggc gatcaggaaa tcttcactcg tgaagagatg
    48001 atcaaatact tcactttgaa tgccgtcagc aaatctgcca gtgcgttcaa caccgacaag
    48061 ctgctgtggc tgaaccatca ctacattaac gcgctgccgc cggagtatgt tgctactcac
    48121 ttacagtggc acattgagca ggaaaatatc gatacccgta acggcccgca gctggctgat
    48181 ctggtgaaac tgctgggcga acgctgcaag acgctgaaag agatggcaca gagctgccgt
    48241 tatttctacg aagattttgc tgagttcgat gccgatgcgg cgaaaaaaca tctgcgtccg
    48301 gtagcgcgtc agccgctgga agtggttcgt gacaaactgg ccgcgattac tgactggacc
    48361 gctgaaaacg ttcatcacgc tattcaggcg acggcggatg agctggaagt gggtatgggt
    48421 aaagttggta tgccgctgcg tgtcgccgta accggtgcgg ggcagtctcc agcactggat
    48481 gttaccgttc acgcaattgg taagacccgc agtatcgagc gtatcaacaa agcgctggat
    48541 tttattgctg aacgcgaaaa tcagcagtaa ttagcgtgta aaaagataaa cggcaggaga
    48601 taatatcctg ccgtttttta tttatgctga ttcgtcaata tcgagacggg cgaggaaatt
    48661 gcgaattcct tcacgtgaca aaaattgcgc gactttttcc tgctcaatgt ctgtcatatt
    48721 atctatcgcg ttgatgattt gtcgatacgc cggggcgcgt gctccaggag cgtattcagc
    48781 aattctctct tcagcttcca tcaacattgg tgtgttatgg aaggctggcg tgttctgaat
    48841 aaactcgcaa acttgcgtat cgaccagaat aagtctggca cgaccgcctt tcacgccggg
    48901 aaatttttcc gttttccagc ctttttctct gatccaacgg ttaacagttt gtttagccac
    48961 accgaggcat tcagccagtt cttcggtggt cattttgttg cgtaatcttt tcatattatt
    49021 tgctatcgcg aatccctaag cgttgtaaca atccgataat cccttcccgc agaagcaagg
    49081 atgtaaactg tttttgttca actggcgtca tctctttcgc cagtgtcacc agcaatactt
    49141 caagcggcgc atcaccagaa agggcaggcg cttctccctg accttctgga cgttcagcat
    49201 tgcgaatata ttcacgaacc tgttcattga cgtgaaccag tcgggctttg ccaccctgga
    49261 cgccaggttt tggtgacgtt gtccagcctt ccttgcgtac ccatttatta atggtctggc
    49321 ggctatagcc ggtcagtctg gcaagttcat ctggtgtcat cctctccttg aacataacaa
    49381 ttccctgaat gtatgtgggt ctattagtag ttcattttat aacaccactt tacaggaaac
    49441 cgtgacatga ataactactg aatgcgagcg gttttccaaa attgttcatt tgcctgattt
    49501 ttcagcgatt gaaactacac gttgcaattt gccgttgaca cattcgggcg gaattcatat
    49561 gatgccgccc gtcaactcga caagctttac gtgacggggc tatagctcag ctgggagagc
    49621 gcttgcatgg catgcaagag gtcagcggtt cgatcccgct tagctccacc aaattttgca
    49681 cccagcaaat ttggtacgta aacgcatcgt ggggctatag ctcagctggg agagcgcttg
    49741 catggcatgc aagaggtcag cggttcgatc ccgcttagct ccaccaaatt tccaaccctc
    49801 gctgcaaagc gggggttttt tgtctctgct ttttgccgct tttgtaatac agtctacgtc
    49861 cgggttagcg ccgcctggtg aaagcatcat tggatgaaaa atcggcaaca ggctggcccc
    49921 tgtttgcttc gcgatgcgaa taaacttatt atttgtgtgc ctgaaaaccc cgatcagtga
    49981 gagtagtgta ctcatgtttg tggagcataa cctgataaaa aatatcaaga tattcacact
    50041 agcgtttacg ctcaccgtgg tacttattca gctatcccgt tttatttcgc cacttgccat
    50101 tatccattcc agttatatct ttctggcgtg gatgccactg tgcgtaatgc tgtcaatctt
    50161 gtttatcttt ggctggcgcg gtgtcgttcc cgttttatgc gggatgtttt gcaccaatct
    50221 gtggaacttt catctctctt ttttacagac tgcggtcatg ctcggtagcc agacgtttgt
    50281 cgtgttgtgt gcctgcgcaa tattacgctg gcagctgggg acgcgttggc gttatggatt
    50341 gaccagccga tatgtctggc aacgtctgtt ctggcttggt ttggtgacgc cgatcggcat
    50401 caaatgcagc atgtatcttg tggggagctt ctttgatttc ccgctaaaga tatccacctt
    50461 tttcggcgat gcggatgcca ttttcacggt cgttgatttg ctaagccttt tcaccgctgt
    50521 gctgatttac aacatgcttt tctactatct cactcgcatg attgtaagtc cccactttgc
    50581 gcagatattg tggcgcaggg atatcgctcc gtcgttgggc aaagagaaac gcgcatttac
    50641 cttaagctgg ctggcagcgt taagcgtgct gctacttctg ttgtgcacac cttatgaaaa
    50701 cgactttatt gccggttact tggtacccgt tttcttcatc atctttaccc tcggggtcgg
    50761 taagcttcgc tatccgtttt taaatctcac ctgggctgtt tcaacgcttt gcctgctgaa
    50821 ttacaaccag aactttttgc aaggggtgga aaccgaatat tcgctggcat ttattcttgc
    50881 ggtgctgatt tcctttagcg tttgcctgct ctatatggtg cgcatttatc atcgcagtga
    50941 atggcttaat cgccgctggc atttgcaggc gctgacagat ccgttaacgc tcctacccaa
    51001 ctttcgtgcg ttggaacaag cgccggagca agaggcgggc aagagttttt gctgcctgcg
    51061 cattgataat cttgagttta tgagtcgtca ttacggctta atgatgcgcg ttcactgtat
    51121 ccgctcaatt tgccgtacgc tgctgccgtt gatgcaggaa aacgaaaagt tgtatcaatt
    51181 gccgggtagt gaactgctgt tagtgctgag cgggccggaa acggaagggc gactccagca
    51241 tatggttaac atcctgaata gtcggcaaat tcactggaac aataccgggc tggatatggg
    51301 ctatggtgct gcctgggggc gttttgatgg aaatcaggaa accctgcaac ccttgttggg
    51361 gcagttaagc tggctggcgg agcaatcctg cgcacatcat catgtgctgg cgctggatag
    51421 cagagaggag atggtttccg ggcagaccac taaacaggtg ctattgctga ataccattcg
    51481 cacggcgtta gatcagggtg atttgctgct ctacgcccag ccaattcgca acaaagaggg
    51541 tgaaggttat gatgagatcc tcgcgcgact gaaatatgac ggcggcatta tgaccccgga
    51601 taagtttctg ccccttattg ctcagtttaa ccttagcgcg cgttttgatt tgcaagtgct
    51661 ggaatccttg ttgaagtggc tggcaacaca cccttgcgac aaaaaaggac cgcgcttttc
    51721 agtcaattta atgccgctca cgctgctgca aaagaatatt gccgggcgga ttattcgtct
    51781 gtttaagcgt tatcacatct ccccgcaggc ggtcattctt gagatcaccg aggagcaggc
    51841 gttttctaac gcagaaagca gcatgtacaa catcgagcag ctgcataagt ttggtttccg
    51901 gattgcgatt gatgactttg gcaccggata tgccaactac gaacggttaa agcgtttgca
    51961 ggctgatatc atcaaaattg atggcgtctt tgtgaaagat attgtcacga acacgctgga
    52021 tgcgatgatt gtgaaatcaa ttaccgatct ggcgaaagcg aagtcattga gtgtggtcgc
    52081 ggagtttgtc gagacgcaac agcagcaggc gctattgcat aagctcgggg tgcaatatct
    52141 gcaagggtat ttgattggtc gcccgcagcc attagctgat taacaggcgt aaaaaagccg
    52201 gggaatgatc cccggctttt ttatgtatgg ttttacagca ccagtgctgc gattgacgca
    52261 gacagcacac tcaccagggt agagccgtaa accagtttca gaccgaagcg agaaaccacg
    52321 ttaccttgct cttcattcag gcctttaact gcacctgcga taatcccgat tgaagagaag
    52381 ttagcgaagg aaaccaggaa cacagagatg atgccttcag cacgcggaga gagcgtggaa
    52441 gcaattttct gcagatccat catcgcaacg aactcgttgg aaaccagttt ggtcgccatg
    52501 atactgccca cttgcagtgc ttcactggaa ggaacaccca tcacccatgc aatcggatag
    52561 aagatgtagc ccaggatgcc ctggaaggag atgctgtagc caaaccagcc agtcacagta
    52621 gcaaacagtg cgttcagtgc ggcgatcagg gcgataaagc cgatcagcat cgcggcaacg
    52681 ataatggcaa ccttgaaacc tgccagaatg tattcaccca gcatttcgaa gaagctctga
    52741 ccttcgtgca ggttggacat ctgaatgttt tcttcactgg catcaacacg gtaagggttg
    52801 atcagcgaca gcacgataaa ggtgctgaac atgttcagta ccagcgccgc aacgacatat
    52861 ttcggctcca gcatggtcat gtatgcacca acgatggaca tagatacggt ggacatcgcc
    52921 gttgctgcca tggtgtacat acgattgcgg gagattttgc cgaggatatc tttataggca
    52981 ataaagtttt cagactgacc cagaatcagg gagctgacgg cgttaaagga ttccagtttg
    53041 cccatgccgt tgactttgga gagcaggaaa ccaattgcgc ggataatcac cggcaacacg
    53101 cgaatgtgct ggagaatacc gatcagtgca gagataaaga cgattgggca cagcactttc
    53161 aggaagaaga atgccaggcc ttgatcattc atgctaccaa agacgaagtt agtcccttcg
    53221 ttggcaaatc cgagcagttt ttcgaacatt tcggagaagc ctttcacgaa gcctaaacca
    53281 acgtcggagt tcaggaagaa ccacgccagt aacacttcga taacaagcag ttgaataaca
    53341 taacggatac gaattttttt gcggtcgctg cttaccagca gtgcgagaat cgcaacaacg
    53401 gcaagtgcca gtacaaaatg aaggacgcgg tccatatttg ctccaaatat gaggcaggtt
    53461 aaatttccgt gcacattcta tgtaacatgg ttaaggaaaa cgagatctga cacacactat
    53521 aatgacgtcc tgtgacaagc ttcaaaaata gtgatctgtc atacatttct gctatgttgt
    53581 gtatggaagc tgaaagttat gtaaatgtgc taacattaaa cataaccata acattcaagt
    53641 catcaatctt ttcgtagtca taatcctggt ctatcaaaga aatcaccaca acccatttaa
    53701 atgaatttga taatcattct cgtttggcat agcatgaaac atagcaaagg ctatgtttta
    53761 gaggcacaag atgacgaact atcgcgttga gagtagcagc ggacgggcgg cgcgcaagat
    53821 gaggctcgca ttaatgggac ctgcgttcat tgcggcgatt ggttatatcg atcccggtaa
    53881 ctttgcgacc aatattcagg cgggtgccag cttcggctat cagctactgt gggttgtcgt
    53941 ttgggccaac ctgatggcga tgctgattca gatcctctct gccaaactag ggattgccac
    54001 cggtaaaaat ctggcggagc agattcgcga tcactatccg cgtcccgtag tgtggttcta
    54061 ttgggttcag gcagaaatta ttgcgatggc aaccgacctg gcggaattta ttggtgcggc
    54121 gatcggtttt aaactcattc ttggtgtctc gttgttgcag ggcgcggtgc tgacggggat
    54181 cgcgactttc ctgattttaa tgctgcaacg tcgcgggcaa aaaccgctgg aaaaagtgat
    54241 tggtgggtta ctgttgtttg ttgccgcggc ttacattgtc gagttgattt tttcccagcc
    54301 taacctggcg cagctgggta aaggaatggt gatcccgagt ttacctactt cggaagcggt
    54361 cttcctggca gcaggcgtgt taggggcgac gattatgccg catgtgattt atttgcactc
    54421 ttcgctcact cagcatttac atggcggttc gcgtcaacaa cgttattccg ccaccaaatg
    54481 ggatgtggct atcgccatga cgattgccgg ttttgtcaat ctggcgatga tggctacagc
    54541 tgcggcggcg ttccactttt ctggtcatac tggtgttgcc gatcttgatg aggcttatct
    54601 gacgctgcaa ccgctgttaa gccatgctgc ggcaacggtc tttgggttaa gtctggttgc
    54661 tgccggactg tcctca
//
LOCUS       DAZFFI010000022        51222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFFI010000022 DAZFFI010000000
VERSION     DAZFFI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.022"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..140
                     /locus_tag="ACHJ12_001574"
     CDS             6..140
                     /locus_tag="ACHJ12_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4989380.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            119..223
                     /gene="speFL"
                     /locus_tag="ACHJ12_001575"
     CDS             119..223
                     /gene="speFL"
                     /locus_tag="ACHJ12_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4989381.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            534..2732
                     /gene="speF"
                     /locus_tag="ACHJ12_001576"
     CDS             534..2732
                     /gene="speF"
                     /locus_tag="ACHJ12_001576"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4989382.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2729..4048
                     /gene="potE"
                     /locus_tag="ACHJ12_001577"
     CDS             2729..4048
                     /gene="potE"
                     /locus_tag="ACHJ12_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4989383.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4104..5744)
                     /gene="pgm"
                     /locus_tag="ACHJ12_001578"
     CDS             complement(4104..5744)
                     /gene="pgm"
                     /locus_tag="ACHJ12_001578"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4989384.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5770..6315)
                     /gene="seqA"
                     /locus_tag="ACHJ12_001579"
     CDS             complement(5770..6315)
                     /gene="seqA"
                     /locus_tag="ACHJ12_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4989385.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6500..7264
                     /gene="ybfF"
                     /locus_tag="ACHJ12_001580"
     CDS             6500..7264
                     /gene="ybfF"
                     /locus_tag="ACHJ12_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4989386.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7404..7697
                     /gene="ybfE"
                     /locus_tag="ACHJ12_001581"
     CDS             7404..7697
                     /gene="ybfE"
                     /locus_tag="ACHJ12_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4989387.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7837..8367
                     /gene="fldA"
                     /locus_tag="ACHJ12_001582"
     CDS             7837..8367
                     /gene="fldA"
                     /locus_tag="ACHJ12_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4989388.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8577..8663
                     /gene="uof"
                     /locus_tag="ACHJ12_001583"
     CDS             8577..8663
                     /gene="uof"
                     /locus_tag="ACHJ12_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4989389.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8656..9102
                     /gene="fur"
                     /locus_tag="ACHJ12_001584"
     CDS             8656..9102
                     /gene="fur"
                     /locus_tag="ACHJ12_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4989390.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9186..9512)
                     /gene="chiQ"
                     /locus_tag="ACHJ12_001585"
     CDS             complement(9186..9512)
                     /gene="chiQ"
                     /locus_tag="ACHJ12_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4989391.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9562..10968)
                     /gene="chiP"
                     /locus_tag="ACHJ12_001586"
     CDS             complement(9562..10968)
                     /gene="chiP"
                     /locus_tag="ACHJ12_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO4989392.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11414..13078)
                     /gene="glnS"
                     /locus_tag="ACHJ12_001587"
     CDS             complement(11414..13078)
                     /gene="glnS"
                     /locus_tag="ACHJ12_001587"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4989393.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13281..15227)
                     /gene="nagE"
                     /locus_tag="ACHJ12_001588"
     CDS             complement(13281..15227)
                     /gene="nagE"
                     /locus_tag="ACHJ12_001588"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4989394.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15560..16360
                     /gene="nagB"
                     /locus_tag="ACHJ12_001589"
     CDS             15560..16360
                     /gene="nagB"
                     /locus_tag="ACHJ12_001589"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4989395.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16420..17568
                     /gene="nagA"
                     /locus_tag="ACHJ12_001590"
     CDS             16420..17568
                     /gene="nagA"
                     /locus_tag="ACHJ12_001590"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4989396.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17577..18797
                     /gene="nagC"
                     /locus_tag="ACHJ12_001591"
                     /gene_synonym="nagR"
     CDS             17577..18797
                     /gene="nagC"
                     /locus_tag="ACHJ12_001591"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4989397.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18845..19597
                     /gene="nagD"
                     /locus_tag="ACHJ12_001592"
     CDS             18845..19597
                     /gene="nagD"
                     /locus_tag="ACHJ12_001592"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4989398.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19854..21518
                     /gene="asnB"
                     /locus_tag="ACHJ12_001593"
     CDS             19854..21518
                     /gene="asnB"
                     /locus_tag="ACHJ12_001593"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4989399.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILTLYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21899..21975
                     /locus_tag="ACHJ12_001594"
     tRNA            21899..21975
                     /locus_tag="ACHJ12_001594"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21933..21935,aa:Met,seq:cat)
     gene            21984..22068
                     /locus_tag="ACHJ12_001595"
     tRNA            21984..22068
                     /locus_tag="ACHJ12_001595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22018..22020,aa:Leu,seq:tag)
     gene            22092..22166
                     /locus_tag="ACHJ12_001596"
     tRNA            22092..22166
                     /locus_tag="ACHJ12_001596"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22124..22126,aa:Gln,seq:ttg)
     gene            22182..22258
                     /locus_tag="ACHJ12_001597"
     tRNA            22182..22258
                     /locus_tag="ACHJ12_001597"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22216..22218,aa:Met,seq:cat)
     gene            22307..22381
                     /locus_tag="ACHJ12_001598"
     tRNA            22307..22381
                     /locus_tag="ACHJ12_001598"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22339..22341,aa:Gln,seq:ctg)
     gene            complement(22535..23710)
                     /gene="ubiF"
                     /locus_tag="ACHJ12_001599"
     CDS             complement(22535..23710)
                     /gene="ubiF"
                     /locus_tag="ACHJ12_001599"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4989400.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23856..25280
                     /gene="miaB"
                     /locus_tag="ACHJ12_001600"
     CDS             23856..25280
                     /gene="miaB"
                     /locus_tag="ACHJ12_001600"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4989401.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25433..26473
                     /gene="ybeZ"
                     /locus_tag="ACHJ12_001601"
     CDS             25433..26473
                     /gene="ybeZ"
                     /locus_tag="ACHJ12_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4989402.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26470..26937
                     /gene="ybeY"
                     /locus_tag="ACHJ12_001602"
     CDS             26470..26937
                     /gene="ybeY"
                     /locus_tag="ACHJ12_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4989403.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27027..27905
                     /gene="corC"
                     /locus_tag="ACHJ12_001603"
     CDS             27027..27905
                     /gene="corC"
                     /locus_tag="ACHJ12_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4989404.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27930..29468
                     /gene="lnt"
                     /locus_tag="ACHJ12_001604"
     CDS             27930..29468
                     /gene="lnt"
                     /locus_tag="ACHJ12_001604"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4989405.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29546..31423)
                     /locus_tag="ACHJ12_001605"
     CDS             complement(29546..31423)
                     /locus_tag="ACHJ12_001605"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO4989406.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWQRARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQIDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            31878..32786
                     /gene="gltI"
                     /locus_tag="ACHJ12_001606"
     CDS             31878..32786
                     /gene="gltI"
                     /locus_tag="ACHJ12_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4989407.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            32956..33696
                     /gene="gltJ"
                     /locus_tag="ACHJ12_001607"
     CDS             32956..33696
                     /gene="gltJ"
                     /locus_tag="ACHJ12_001607"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4989408.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            33696..34370
                     /gene="gltK"
                     /locus_tag="ACHJ12_001608"
     CDS             33696..34370
                     /gene="gltK"
                     /locus_tag="ACHJ12_001608"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4989409.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34370..35095
                     /gene="gltL"
                     /locus_tag="ACHJ12_001609"
     CDS             34370..35095
                     /gene="gltL"
                     /locus_tag="ACHJ12_001609"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4989410.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35213..36148
                     /gene="rihA"
                     /locus_tag="ACHJ12_001610"
     CDS             35213..36148
                     /gene="rihA"
                     /locus_tag="ACHJ12_001610"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4989411.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36232..37902
                     /gene="hscC"
                     /locus_tag="ACHJ12_001611"
     CDS             36232..37902
                     /gene="hscC"
                     /locus_tag="ACHJ12_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4989412.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(37962..38681)
                     /gene="djlC"
                     /locus_tag="ACHJ12_001612"
                     /pseudo
     CDS             complement(37962..38681)
                     /gene="djlC"
                     /locus_tag="ACHJ12_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(38809..39546)
                     /locus_tag="ACHJ12_001613"
     CDS             complement(38809..39546)
                     /locus_tag="ACHJ12_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO4989413.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRANVHKFRQFRYIRFLNKRAK
                     KRLNTKQFKVQPYPKPQTVKTGLKESE"
     gene            complement(39500..39700)
                     /locus_tag="ACHJ12_001614"
     CDS             complement(39500..39700)
                     /locus_tag="ACHJ12_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4989414.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            39815..40281
                     /locus_tag="ACHJ12_001615"
                     /pseudo
     CDS             39815..40281
                     /locus_tag="ACHJ12_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40320..40565)
                     /locus_tag="ACHJ12_001616"
     CDS             complement(40320..40565)
                     /locus_tag="ACHJ12_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4989415.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(40601..40783)
                     /locus_tag="ACHJ12_001617"
     CDS             complement(40601..40783)
                     /locus_tag="ACHJ12_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4989416.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            complement(40930..42939)
                     /locus_tag="ACHJ12_001618"
     CDS             complement(40930..42939)
                     /locus_tag="ACHJ12_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4989417.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENGVNVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSAWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAVTT
                     TLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGKSSWYLDHDAG
                     TGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDAGNNLLASFYI
                     QTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQVIPVIDGQDGA
                     AFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            complement(43039..43599)
                     /locus_tag="ACHJ12_001619"
     CDS             complement(43039..43599)
                     /locus_tag="ACHJ12_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4989418.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(43613..43798)
                     /locus_tag="ACHJ12_001620"
     CDS             complement(43613..43798)
                     /locus_tag="ACHJ12_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989419.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISTLKGFSTSF"
     gene            44155..44676
                     /locus_tag="ACHJ12_001621"
     CDS             44155..44676
                     /locus_tag="ACHJ12_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4989420.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(44711..45622)
                     /locus_tag="ACHJ12_001622"
     CDS             complement(44711..45622)
                     /locus_tag="ACHJ12_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4989421.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYIHNTWRTVSSV"
     gene            complement(45622..46182)
                     /locus_tag="ACHJ12_001623"
     CDS             complement(45622..46182)
                     /locus_tag="ACHJ12_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO4989422.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            complement(46173..47255)
                     /locus_tag="ACHJ12_001624"
     CDS             complement(46173..47255)
                     /locus_tag="ACHJ12_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4989423.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(47255..47692)
                     /locus_tag="ACHJ12_001625"
     CDS             complement(47255..47692)
                     /locus_tag="ACHJ12_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4989424.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(47685..48299)
                     /locus_tag="ACHJ12_001626"
     CDS             complement(47685..48299)
                     /locus_tag="ACHJ12_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4989425.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNITVDKNLHVKGNV
                     SIDGTGRSQGAFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(48289..49413)
                     /locus_tag="ACHJ12_001627"
     CDS             complement(48289..49413)
                     /locus_tag="ACHJ12_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4989426.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(49397..50746)
                     /locus_tag="ACHJ12_001628"
     CDS             complement(49397..50746)
                     /locus_tag="ACHJ12_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO4989427.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLTEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            complement(50733..>51222)
                     /locus_tag="ACHJ12_001629"
     CDS             complement(50733..>51222)
                     /locus_tag="ACHJ12_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO4989428.1"
                     /translation="RAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAA
                     PVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTE
                     AWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYG
                     LW"
BASE COUNT        12414 a        13358 c        12805 g        12645 t
ORIGIN      
        1 aattaatgat ttattatttg gggtatgatc gttttttgtt gatcttcttc acagattata
       61 gccatttcat ggatagaata actctacctt caactgacac agcaagaggt aaaggtaaat
      121 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc
      181 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
      241 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
      301 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
      361 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
      421 caaactactg atttatttat cagcggtgga gctttgcttt ttttccggcg tgatcgattt
      481 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaac aaaatgtcag
      541 aattaaaaat tgccgttagt cgttcttgcc ctgattgttt ttccactcat cgtgcatgcg
      601 tgaatataga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcagtgatg
      661 ttgaacgtgg aaaactcgat gaaatagacg ctactggcta tgacattcct gtttttattg
      721 caacggaaaa tgaagaacgt gtacccgcag aatatctgcc acgtatttct ggtgtcttcg
      781 aacattgcga atcacgtaaa gagttttatg gacgtcagtt agaaaccgct gccagccatt
      841 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtt aatcagggca
      901 atagtgcttt tgactgccct ggtcatcagg ggggtgagtt tttccgtcgc catccggcag
      961 gcaatcagtt cgtggaatac tttggcgaaa tgctcttccg ctctgacttg tgtaatgctg
     1021 acgtagcaat gggcgatctg ctgattcatg aaggtgcgcc atgtattgca caacagcatg
     1081 cggctaaagt ctttaatgcc gataaaacct atttcgtttt aaacggtact tcatcttcta
     1141 ataaagtggt cttaaacgca ttgctgacgc cgggcgatct ggtgttgttt gaccgcaata
     1201 accataaatc taaccaccac ggtgcgttgc tacaggctgg cgcgacaccg gtttatctgg
     1261 aaaccgcacg taacccgtat ggttttatcg gtggtattga cgctcactgt tttgaagaaa
     1321 gttacctgcg tgagctgatt acagaagttg caccgcagcg ggcaaaagag gctcgtcctt
     1381 tccgcctcgc cgttattcag ctaggcactt acgacggtac gatttataac gcccgtcagg
     1441 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
     1501 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
     1561 acgatccggg tattctggtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
     1621 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
     1681 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
     1741 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
     1801 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
     1861 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
     1921 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
     1981 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
     2041 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
     2101 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc
     2161 tgactccggc agaagatatg gccaagcttc agcaacttgt tgccctgctg gtacgcttcg
     2221 aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc
     2281 atgaagagcg ctacgccggt tataccctgc gtcagttgtg tcaagaaatg catgatttgt
     2341 atgcccgtca caacgtgaaa caactgcaaa aagagatgtt ccgtaaggaa cacttcccac
     2401 gcgtaagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg
     2461 ttcgtctgcc ggatgcagaa ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg
     2521 gtgtgctgtg cgttgttccg ggtgaaatct ggggtggtgc tgttctgcgt tacttcagcg
     2581 ctctggaaga agggatcaac ctgttgccag gttttgcacc ggagcttcag ggtgtctata
     2641 tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc
     2701 aaagtaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt
     2761 cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc
     2821 gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg
     2881 ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg
     2941 tatgggcggt tatgccgagt atgctttcgg taagtccggc aattttatgg cgaactacac
     3001 ctacggtgtc tcgctgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg
     3061 caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggagt
     3121 gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag
     3181 cattactgtg tggggggtca ttattccggt ggtaggcctg tgcattatcg gctggttctg
     3241 gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc
     3301 agtaggttct tccatcgcta tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc
     3361 gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac
     3421 gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa
     3481 tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgttcacacc
     3541 agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg
     3601 ctggcagttc accattgccc aggtgtttaa atcttcatct gatgaaggct acttccctaa
     3661 aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat
     3721 tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt
     3781 gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt
     3841 agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt
     3901 tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat
     3961 gctgtacggt tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg
     4021 ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac
     4081 cgcccttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc
     4141 gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta
     4201 tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc
     4261 agaccgccaa tagaagcacc gttgcccgga gcagcagtca ggcgcgcggt gatcgggtca
     4321 cctgccaggg tgctggcgct caccatttcc ggagacagct tagacagcgc cgctttttgt
     4381 gcggaagtcg cagctgcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt
     4441 tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga
     4501 cacatgatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa
     4561 gcccctgcgc tctcttcgcc gccaaaaccg aagctgccgt cgaacagacc atcaacaaac
     4621 catttgaaac ctaccggtac ttctaccagc ttacggccca agtcattgac cacacggtcg
     4681 atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga
     4741 tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc
     4801 ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta
     4861 tcacgcagtg ccagcaggcc cgccatcgca cactcggagg agcagtccat acggatcgcg
     4921 ccgtctttat caaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc
     4981 aggttgaggt tgtaatactc gccaatacgc ttccagtatt cgataccgga accgcccagc
     5041 ggatcaacgc caagcgtcag gcccgctttc tgaatcgccg ccatatcaac gatatcggcc
     5101 agcccttcca cgaacggctg caccagatcc tgctctttca catgaccgga tgccatcgct
     5161 tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccagcagtgc gttggcacga
     5221 tcttccacca ctttagtgac gttggtatca gccgggccac catttggcgg attgtacttg
     5281 ataccaccat cttccggcgg gttatgggac ggtgtaataa cgataccgtc tgccagcggg
     5341 ccaccttttt tattgtgaac caggatggca ttggaaacgg caggcgtcgg ggtgaagcca
     5401 ttgttttcct gcacaataac atctacgccg ttcgctgcca gcacttccag aacggaaata
     5461 aatgcaggtt cggacagggc gtgagtatct ttacccacat agcaagggcc agtgataccg
     5521 tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag
     5581 ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca
     5641 ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa
     5701 tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc
     5761 tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga
     5821 ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca
     5881 atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg
     5941 ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa
     6001 cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg
     6061 cttttgctct gcgtattcat ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt
     6121 aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt
     6181 caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga
     6241 tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac
     6301 ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaggagt
     6361 atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata
     6421 aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg
     6481 cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca
     6541 caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt
     6601 actggctcgc gatctggtaa atgatcacaa tatcatccag gttgatatgc gtaaccacgg
     6661 tctttcaccg agagatccgg tgatgaatta cccgacgatg gcgcaggatc ttgttgatac
     6721 tctggatgca ctgcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc
     6781 ggtaatggca cttactgcac tggcccccga tcgcatcgat aaactggtgg cgattgacat
     6841 cgcgccggtc gactatcacg tacgccgtca tgatgagatt ttcgcagcta tcaacgcggt
     6901 cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcgcc agcatcttaa
     6961 tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa
     7021 cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg
     7081 ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg
     7141 tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca
     7201 ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga
     7261 ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt
     7321 gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg
     7381 tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc
     7441 tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac
     7501 agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg
     7561 tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gagtcgcgca
     7621 atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta
     7681 gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg
     7741 ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac
     7801 ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc
     7861 ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa
     7921 gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttatgac
     7981 attctgctgc tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac
     8041 ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct gtttggttgt
     8101 ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc
     8161 attgaaccgc gcggtgcaac catcgttggt cactggccaa ctgcgggcta tcatttcgaa
     8221 gcatcaaaag gtctggcaga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt
     8281 cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg
     8341 catctcgacg aaattcttaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta
     8401 ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct
     8461 gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt
     8521 tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga
     8581 tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa
     8641 caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta
     8701 acgcttccac gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt
     8761 gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta
     8821 tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc
     8881 ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat ttgcctcgac
     8941 tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc
     9001 gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa
     9061 ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa
     9121 agccaacctg cgggttggct tttttatgca agggaaataa gaaaagcgag agttacagct
     9181 ctcacttatt tgttattact gcgaatttcc tgccaaactt tatcacaatc ggctttcacc
     9241 gcctggtcat taccggtttg cgttgcgcgc agacactgct gataatccag tacgcgtaca
     9301 ctttcctggt cggcaaactg ctgatgttgt ttttctttct tcagcacgtt taacacgctc
     9361 tggcaggctt caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc
     9421 tctttcagac gactgtcttc ctgtggcgca gttgattgcg cacaagctac cagccccgat
     9481 gccatcatgg cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca
     9541 caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac
     9601 gtcacgctca tcctggaaga tgttaccgta accgccgccc cagcttggga tgtcagagtg
     9661 gttgtcgtat tcggtgaagt gcagtttgaa catcgtgcct ttggcgcggc cgtcctgaat
     9721 ggtgtagacc gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta
     9781 cgcatccgga ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc
     9841 gccgatggcg aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac
     9901 cgctttttct ccgttagcgt tgaagtcgga acggttatcc caccagatat ccaggcgacc
     9961 gttcgaggaa gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc
    10021 cttaacccag gtgccttcaa ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt
    10081 caatgcttgc agccaagcgg tgccgtcata aagatcgttg acgctgcgat cgtcaacttt
    10141 atcgcgggta ccgtagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt
    10201 gtagctggct ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc
    10261 cagtacgaag ttatttttga agtcgtattt cgccccaagg gagtgcagat aatcaacttt
    10321 ggtggtttta tcgttctgat aaaactcatc catttccaga tgccacggcg ctttatattc
    10381 gttggtccac atgtaggaga aactcaaggc accagcatcg ccgtaatcaa aattcgcccc
    10441 ggcttccgcc ccctgataag tacctggcat aaagctccag tgcggcgcta acagcgtttg
    10501 accagttggc tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc
    10561 agctttatac aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt
    10621 actttttgaa aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat
    10681 cgccgtaaac gcagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc
    10741 gaggttggca ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac
    10801 atctttacgg tcgcgttcac gctgccagta atatataccg ccggttaagg ttgaatcgtc
    10861 gatgaatcct tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc
    10921 ggcgatagcc agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc
    10981 tttgacgtat aaattgctgc accaaaggtg aacagcgaaa aaataaaaat taaaaaagta
    11041 gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg cgaattatca
    11101 atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt gatgattttt
    11161 gttacaaggc ttaagtttgc ggttgaaagg cttgcagaag aataaaagcg tgaatttcca
    11221 gcaagatgga agcggttaca ttttatttaa ttatggtttg tgattttaaa tttattttat
    11281 ggataaattg cgaagcggaa ttaattcgcg aagaaaagca gaaaaaacgc cgcagagtgc
    11341 ggcgtttggg attgtttgct atttcttgcc ggatgcgacg taaatgcctc acccggcctg
    11401 gtagaactta aaattactcg cctactttcg cccaggtatc acgcagccca acggtgcggt
    11461 taaataccgg tttttccgct gtggaatggc ggctgtcgag gcagaagtaa ccttcacgct
    11521 caaactggaa tgctttaccc gcaaccgcat ctttcagcga cggttcagca aagccctgtt
    11581 tgatcaccag cgattccggg ttaatcaccg acaggaaatc atccgcagca ccagggttcg
    11641 gcacgctgaa cagacggtcg tacagacgga tttcaaccgg cagcgcatgt gccgcgctca
    11701 cccagtgaat aacacctttg actttacgac catctgccgg atctttgctt aaggtgtcgg
    11761 cgtcataagt acagaagatg gtggtgatat tgccttcggc atctttctcc acgcgttcag
    11821 ccttaatgac gtaagcatta cgcagacgca cttctttacc cagcaccaga cgtttgtact
    11881 gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca aatctcaccg ctaaacggca
    11941 cctgacgact ccccatttcc ggtttgttcg gatggttcgg catggtgacc atttcgcctt
    12001 cgccctgata gttttcgata accagtttca ccggatcgat aaccgccatt gcgcgcggcg
    12061 cattttcgtt gagatcttca cggatgcagg attccagcga tgccatctca atggtgttgt
    12121 cctgcttggt cacgccgatg cgtttgcaga actcacgaat agaagcagca gtgtaaccac
    12181 gacgacgcag accggaaatg gtcggcatac gcgggtcatc ccagccttca acgtgcttgt
    12241 cggtcaccag caggttcagc ttacgcttgg acatcacagt gtattccaga ttcaggcgcg
    12301 agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt gtccagcacc cagtcgtaca
    12361 ggcgacggtt gtcctggaac tcaagcgtac acagagagtg cgtaatacct tccagggcat
    12421 cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca ccacttgttg ccagtctggt
    12481 ggtgttcagc aaatttaata cggtacagca ccggatcgcg catcacgata aacggtgaag
    12541 ccatgtcgat tttcgcacgc aggcaggctt taccttcttc aaaaccaccg gcacgcattt
    12601 tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg
    12661 gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg cgtcagttca tcaacgtacg
    12721 ccaggccttt attgatcagt tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg
    12781 agtaacggac gttaccagac cagtgaaaac ctaaccactc tacgtcgttt ttgatcgact
    12841 caacatactc gatatcttct tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc
    12901 ctttatagtc ctgggcgatc ccgaagttca ggcagataga tttcgcatgg ccaatatgca
    12961 gatagccatt cggctccggc gggaaacggg tgtgtactgt ggtgtgctta ccactggcca
    13021 gatcttcatc gatgatctga cggataaagt tagtcgggcg ggcttctgcc tcactcatcg
    13081 tggattcctc aaagcgtaaa caacgtataa cggcgtatga tcttataagc gggacaggct
    13141 gacaactgtt agttttttaa aattgataat ctaacaagga ttatggggat gattgatgaa
    13201 aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt tggatgcgac gctcaagcgt
    13261 cgcatcaggc ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca
    13321 acatggccct gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc
    13381 gggctaatca tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt
    13441 tgccctgcgc ttacctgcgc accctcttcc accagacgtt taaagccttt accttccagc
    13501 gctacggtgt cgatacccat atggacgacg atctccgcgc ctttttcggt ttccaggcag
    13561 aacgcgtggt tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt
    13621 ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca
    13681 ggaacctgat ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat
    13741 accgcgtttg gtacagcctg cggttttgct acaggcgcgg cagttgccgg agttgcttca
    13801 gctgacgcag ctgctaccgg accgcgggca acgactttct tcatcgcatc gccgatggat
    13861 tctgctttcg cgccaacaat cacctgaata gtctgtttgt tcagttttac tacaccagaa
    13921 gcacccagac gtttacacat cgtatcgtta acgcgggcag agtcagccac tgtaaggcgc
    13981 agacgggtga tacaggcgtc aattgctttc aggttgtcag tgccgccaac cgcagcaata
    14041 tagttggtcg ccagttgatt gaggccttct tcagtgttgc tgttggcttc ttcagtaacg
    14101 atctcgtctt ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa
    14161 ctgaacacca cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg
    14221 ttctggctgg cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaaa
    14281 gagaagccag catggatacc cagcagtgtt gccacaaaca ggctgatacc ggtcagcagt
    14341 gcgtgcagga ggtacagcag cggtgcaagg aacatgaaca ggaattccag cggctcagtc
    14401 acaccggtca ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga
    14461 cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcata
    14521 atcgggaaga agccggacat gaacatcccc gcggtgccgt caccggcata gaagcggttg
    14581 atatcaccgt ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg
    14641 atggtgttca gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag
    14701 ataccggaac ccagcgcgcc cgcagaaacg atccactcgc ctcctgcatg gatagcgtgc
    14761 tgtaccggcg gccagacgta accaaaaatg gccgccagca ccaggcagaa gaagccggtg
    14821 gcaatcggca caaagcgttt gccgccgaag aaactcagga agtccggcag tttaatatcg
    14881 gaccaacggt tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc
    14941 atgttaattt ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca
    15001 cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg
    15061 aagattaatg cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta
    15121 agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg
    15181 agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta
    15241 cgagaaccct atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc
    15301 gatgttctgt aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt
    15361 atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc
    15421 ccttttactg gctaaaccag aaaacttatt ttatcattca aaaaatcagg tcggattgac
    15481 gcctgtctgc gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt
    15541 ttacttattg aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa
    15601 atgggctgct cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc
    15661 gtttgttctg ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga
    15721 aatgcataaa gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata
    15781 tgtcggtctg ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt
    15841 cgatcacgtt gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat
    15901 cgacgccgaa tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt
    15961 tatgggcggt gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc
    16021 ttctcgtact cgtatcaaaa ccttgactca tgacactcgc gtcgcgaact ctcgtttctt
    16081 tgataacgat gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct
    16141 ggatgccgaa gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc
    16201 tgcagttgaa ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa
    16261 agcgatcatg gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata
    16321 tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct
    16381 ccttgctcag ggcaatattt ttaaaaatcg ggggtcagaa tgtatgcatt aacccagggc
    16441 cggatcttta ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg
    16501 attaaaagcg tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac
    16561 ggggccattc tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag
    16621 tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat
    16681 gagaaatcag gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg
    16741 aaacagggcg tgcgcgttat gcgcgagtac ctggcaaaac atccgaatca ggcgttaggt
    16801 ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt
    16861 gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc
    16921 aaagtgaccc tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc
    16981 gggattgtgg tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc
    17041 cgcgcgggga ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt
    17101 gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct
    17161 gatggcctgc atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa
    17221 ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt
    17281 gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc
    17341 ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca
    17401 ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa
    17461 cgtctcggca cactcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt
    17521 aaaatcacca agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga
    17581 caccaggcgg acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg
    17641 cggtttatcg cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa
    17701 gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga
    17761 tcaaagaagt tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg
    17821 aaacccgcaa tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accatcaccc
    17881 tgtttgatct cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc
    17941 agcaaacgct ggaacatgcc ctgttgaatg ccattgctca gtttattgat agctaccagc
    18001 gcaaactgcg cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt gacccggaca
    18061 gcggcaaaat tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac
    18121 tggaagaacg ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg
    18181 cggagcacta cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc
    18241 gcggaaccgg ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggcaacg
    18301 tcggtgaaat tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact
    18361 ttggctgcct ggaaactatc gctgccaacg ccgctattga acaacgggtg ttgaatctgt
    18421 taaagcaggg ctaccagagc cgcgtgccgc tggacgactg caccatcaaa actatctgca
    18481 aagccgcgaa caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc
    18541 tgggcaaaac catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgccg
    18601 gtgaaatcac cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc
    18661 aggcgctgaa ggcgtttcgc actaatctgc cggtggtacg ttctgagctg gatcaccgct
    18721 cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc
    18781 atttgctgga aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta
    18841 gtccatgacc attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt
    18901 cgccgtaccg ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt
    18961 gttgctgacc aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc
    19021 cggtgtcgat gtgccggata gcgtgtttta tacctctgcg atggcgactg ccgatttcct
    19081 gcgtcgccag gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact
    19141 gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat tttgtgattg ttggcgaaac
    19201 gcgttcctac aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg
    19261 ctttatcgcc accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt
    19321 gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc
    19381 gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt
    19441 cggcgataac ttgcgtaccg atattctggc gggcttccag gcgggtctgg agacgattct
    19501 ggtgctttct ggtgtttcgt cgctcgacga tatcgacagt atgccgttcc gcccaagttg
    19561 gatttaccca tcggtcgctg aaatcgacgt tatctgaata gcaaccacgt ctgcggacgt
    19621 ggtttcttat ttttacccat cattaaaatc atcccattca ataaaacccg tcaaaaatta
    19681 tcgatccatc aataaatacg ccaccacacc ctatcctttt tcacaaatca gcaatcacca
    19741 ttacgttttt tattttttca ccgccaaatc gcttgcgcac agtgctgctt tgcggcattt
    19801 ttttaaacaa gcaaacacaa caagcaacaa ataccaagtt aacggagaag gttatgtgtt
    19861 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg
    19921 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
    19981 acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc
    20041 tctacaacca acaaaaaacc cacgtactgg cggtaaacgg tgaaatctac aaccaccagg
    20101 cattgcgcgc tgaatatggc gatcgttatc agttccagac agggtctgac tgtgaagtga
    20161 tcctcacgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg
    20221 cctttgccct gtacgacagc gaaaaagatg cctacctgat tggtcgcgac catctgggga
    20281 tcatcccact gtatatgggc tatgacgaac acggtcagct gtatgtggcc tcagaaatga
    20341 aatcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga
    20401 gccaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga
    20461 aagataacgt gaccgacaaa aacgagctgc gtcaggctct ggaagattcc gttaaaagcc
    20521 atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg tggtctggat tcctcaatta
    20581 tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag
    20641 cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg gatcttaaag
    20701 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
    20761 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
    20821 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
    20881 aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccata
    20941 aagcgcccaa cgctaaagaa ctgcatgaag agacggtgcg taaactgctg gccctgcata
    21001 tgtatgactg cgcgcgcgcc aacaaagcga tgtccgcctg gggcgtggaa gcgcgcgttc
    21061 cgttcctcga caaaaaattc cttgatgtgg cgatgcgtat taacccacag gataaaatgt
    21121 gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcctg
    21181 caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg
    21241 acaccctgaa agaagtggcg gcgcagcagg tttctgatca acagctggaa actgcccgct
    21301 tccgcttccc gtacaacacg ccgacgtcca aagaagcgta tctgtaccgg gagatctttg
    21361 aagaactgtt cccacttccg agcgccgctg agtgcgtacc gggcggtcct tccgtcgcgt
    21421 gttcttccgc taaagcgatt gagtgggatg aggcgttcaa gaaaatggac gatccgtctg
    21481 gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcgaaac agccccggtg
    21541 aaatccgggg ctgtttcagt tattgaggtg cttgttaatt ctgatatgca gtaaggaaat
    21601 gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga
    21661 ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa aagaatggct
    21721 gtagggtggg gaaaaaacac aaagtaaaca ataattgacg aatatagcgc cacgctgttc
    21781 gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt tgacgctgca
    21841 aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa aaaaagatgg
    21901 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    21961 ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca ccagatttag
    22021 gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag
    22081 cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc
    22141 tggttcgaat ccaggtaccc cagccatcga agaaacaatc tggctacgta gctcagttgg
    22201 ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa
    22261 ttctgaatgt gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa
    22321 gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc
    22381 acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat
    22441 gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac
    22501 ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg
    22561 cctgacgttt caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac
    22621 gcagcggcgg cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa
    22681 tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg
    22741 cttcaccgta gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac
    22801 caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg
    22861 caagccccgg ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg
    22921 caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt
    22981 gcgccatatt catattctgc aactggcgaa tacgtaccgg agagtcatac cacaccagcg
    23041 atgcccagtt atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc
    23101 aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatacac gactgcgcat
    23161 actgccaggc atgaacgcca attcccgcca tctgccgcac ctgcgaattt gcgccgtcag
    23221 caccaatcac cagcttcgcg cgaatcactt caccgccctt cagttccagc tcctgaagat
    23281 catcatggcg atgcagcgca atcagtgagc ctggcacacg taacgttact ttcggatgcg
    23341 cttccagcgc ctgccacagc gcctgttgca ggacggtgtt ttccaccata tagccaagca
    23401 gcggcagctt aagttcagcg gcgtcaaaca tcacatgcgc cgtttcccac tcccacgttt
    23461 ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc cctaaccctt
    23521 taagcaatga taccgatgcg gcgctgatcg ccgagatccg cacgtccggt tggctatcag
    23581 cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca
    23641 gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt
    23701 gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc
    23761 ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc
    23821 tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa
    23881 cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca
    23941 cccacggcta tcaactgacc gacgtggcag aagaagcgga tgtgctgctg ctgaacacct
    24001 gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt
    24061 taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag
    24121 gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc
    24181 accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca
    24241 gcttcccgga aatcgagaag tttgaccgtc taccggaacc gcgcgccgaa gggccgaccg
    24301 cgtttgtctc catcatggaa ggctgcaata aatattgtac ctactgcgtg gtgccttaca
    24361 cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg
    24421 ctgctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa cgtgaacgcc tggcgtggtg
    24481 aaaactacga cggcaccacc ggatcgtttg ccgatctgct gcgtctggtt gctgcgatcg
    24541 acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca
    24601 tcgaagtgta ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacaaagcg
    24661 gttccgatcg cattctgaac ctgatgggcc gtactcacac ggcgctggag tacaaagcga
    24721 tcatccgtaa actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg
    24781 gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca
    24841 atttcgacat gagctacagc tttatcttct ctgcacgtcc ggggacgccc gccgccgata
    24901 tggtggatga tgttccggaa gaagagaaga agcagcgtct gtatattctg caagagcgca
    24961 ttaatcagca agcgatggcg tggagccgcc ggatgctcgg caccacccag cgtattctgg
    25021 tagaagggac ttcgcgtaag agcatcatgg agctttccgg gcgtacggaa aataaccgcg
    25081 tggtcaactt cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg
    25141 acgtctaccc gaactctctg cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc
    25201 gcgtggcaga aacaccggaa tcggtgattg cccgtacccg caaagaaaac gaccttggcg
    25261 tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg ttttttctct
    25321 ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct
    25381 tataggccgg tgaatgttca gcttccggcg acatacagat aagaggaacg gtttgaacat
    25441 agacactcgc gaaatcaccc tggagccagc agacaacgcg cgtctgttga gcctgtgcgg
    25501 cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg
    25561 cgacaaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct
    25621 gcgtagcctg tatgtcgata cagccccgat gcgcggtcag attcaggata tcgaaccgga
    25681 acagatccac cttgcgatta aagaagcgcg agtactggag caaagtgcgg agagcgtgcc
    25741 ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc
    25801 aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc
    25861 ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca
    25921 ggagattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt
    25981 cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt acgacgcgct
    26041 gtttgaaatg ctgggctttg agaaagttga gaaactgatt gagcgcaacg ttattgaagt
    26101 cgcgccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga
    26161 gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc
    26221 aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg
    26281 cttacgtcac gccatcgaag tgctggccga tgtcgaagag atcagcttta acttcttcca
    26341 cagcgaagac gtggttcgtc accctgtggt ggcgcgtatc gttaacgcct atgaagcctg
    26401 ggaagaagcc gaacaaaaac gaaaagcggc gctggcggca gaacgcaagc gcgaagaaca
    26461 ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg
    26521 ttaccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa
    26581 gaatcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct gaatctgacc
    26641 tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc
    26701 atggagatgt cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct
    26761 caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg
    26821 catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga agccctcgaa
    26881 acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt
    26941 gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta
    27001 actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga
    27061 taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga
    27121 aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg
    27181 aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca
    27241 tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc
    27301 ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc
    27361 acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag
    27421 ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag
    27481 accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat
    27541 tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactaatt gttggtgaga
    27601 ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct
    27661 ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta
    27721 gcgatgaaga ggttgacact atcggtggtc tggtgatgca ggcatttggg catcttccgg
    27781 cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg gccgacagtc
    27841 ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagccg aagctggatg
    27901 aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc
    27961 gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt
    28021 acgacgtctg gcctgcggcg attatttcgt tgatggggct tcaggcgttg acctttaacc
    28081 gccgtccact ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg
    28141 gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca
    28201 tcttcctggt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg
    28261 tgctgtcgcg tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gccccttctc
    28321 tctggcaagt gaccgagttt ctgcgcggtt gggtactgac aggcttcccg tggttacagt
    28381 tcggctatag ccagattgat ggcccgttaa aagggctggc accgataatg ggcgtggaag
    28441 ccattaactt cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca
    28501 actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca
    28561 tccaatggtt taccccacaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata
    28621 ttccgcaatc actgaaatgg gatggagatc agcttcttaa tacgctgaag atttactaca
    28681 acgcaacggc tccgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa
    28741 ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgcgata
    28801 aaggtagctc gctcgtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg
    28861 atacctacaa caccatcatc acactgggta aaggtgcacc gtacagctac gaatcagccg
    28921 atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc
    28981 tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata
    29041 tccagccgcc gctgtcggta aatggtattg agcttactgc ggccatttgc tacgagatca
    29101 ttcttggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg ctgactatct
    29161 ccaacgatgc gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa
    29221 tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg
    29281 tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttaa
    29341 ccactaatgt gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc
    29401 tgtgggtgct gacggcactg tttggttttg ccgctgtgct gatgagtctg cgagcacgta
    29461 aacgttgatg tgcctgatgt atcctgcatg agcaaaacgt actttctcat tgagctttgg
    29521 ggcatggtta tcatgcccct ttttcttacg gttcagggat acaagccaca ttgccatatt
    29581 ggggatgacg aataattgcc tgcggatcaa gcgagcgcgt cctgtccagt tggatcaacc
    29641 cgttcttcgc cataacaaat atttttctac catccgctga aaaacgaagg tgattaacgt
    29701 agctggcagg ataaacgcca ataatctgcc cggcattaat attgagcagg atgacataac
    29761 cttgcaggcc atatgacacc aacgctaaat actgaccgtc cgggctaata ccctcaacgg
    29821 aataaacgct cctgctacac cattgcgtca taatgctggc ttgttgatcc cctacgctta
    29881 tacgtgaaat cccctggctg tcggcaaaaa ttacatcccc gttttcaggt cgaaaacgca
    29941 tcgccgatat cgttcctcgc gcatcactag ccggatagct ttctttctga tgcgtgatgg
    30001 tattgatgcg tacaatgtca cctctgacca taccgggtat caacaatatt ttttcatccg
    30061 gggaaagagc cataacccag gcaccaggtt tattactcgt ccccgtcccg tcgtatttct
    30121 ccagcgggat atccgccaca tgctgataag tcaccagatc aatcgctgac acactattac
    30181 tggtgcgatt catcacccag gcttttgtac catcacgcga gaggataaga ctgtcaggat
    30241 gtgcaccggt ggtaatcgac tgaataatct cccctgtcgc cacgttaatg accgataacg
    30301 cattcgcttc aaatgaagtc gcataaatca acttttcatc cgggcttaat gccagcgatc
    30361 tgacgccctg atctttacag ccaccacaat gtttgactga cttttctttc gctatgggcg
    30421 ctgtaaaggt atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg ctgttggtgt
    30481 cttcaattgc ggcatacaac gtattaccat cccgactgat cgccatatca tggatatcgg
    30541 caatctcagc gagcaaatca ccactggcct gttcacgact taccggagcg gataatgttt
    30601 ccctgagggc gttgcgttcc tccgctaact gttgtttttt ttgtcgtcgc tcttgttcaa
    30661 tctcctgcgg ataaaactct tcacgcaggc tttgcctgac ttgtaaaact gactcatcaa
    30721 tctgctgctg cgcaagccag atagccccgg tcagaagaag actacccgca acaataatcc
    30781 caccttccgt aacaagccgg ttttgcccag ataaacgcgc tctctgccaa cccaacgcgc
    30841 cacccgcaat caggccgcca atgtggcaag cgttatctat tcctgtttgc cgggcaccgt
    30901 acaacagtgt cagcgccacc atacccaaca gaggaaatac tcgccgctgg tttttatggt
    30961 atgtgcccgt acctgaagca aactgtgttg ctatcgctgc accagctatt cccagaatcg
    31021 ccccggatgc cccaacgctg atgactatct gctcactttc gcgtaacgca taacaggcgc
    31081 tgatgagccc accaaagacg ccggagaata accaggtaat caggagtcgc catttcccca
    31141 gaatctgttc cgccacgcct cccacgacca ggaatgcaaa catattcatc agcagatgag
    31201 aaacactact gtgcagaaag atactgctca ataagcgcca gggttcaccc gacaaagtaa
    31261 gaggcgcaac gtttgcgccg agatggataa gtgcggattg atcgacctct tcataactca
    31321 tccattgtgc gagataacaa aatatcccaa tacagagtgc aaaaaggata agggttattg
    31381 cgggtaattg aacatttaac ggcttaaccg aagatgcaga cataaggtgt ttcttccttg
    31441 aaaagacagg taaaaagcat ttttcccgtg tgcagttatc atcccggcaa acgtcccatg
    31501 ccgaaaagat aattctgtcc gaaatatgac tttttcagcc taaaattaca atcgtaatta
    31561 attcgcccct aagtattttt tggcatgtaa gttgctttat ttactgtgta agcgatgaaa
    31621 tcagccgcga cgcaaccagg ttgcaccacc acaaatcagc ggtagcacca aaaaggtgcg
    31681 gtttaagaat tttgcttcgg aatggtgcgg cacatctcgc aaaaacaaac aataaaacag
    31741 caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa
    31801 agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga
    31861 agataaagga gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc
    31921 tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca
    31981 aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt
    32041 attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg
    32101 aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct
    32161 cacaaaaccg tattccactg ctgcaaaacg gcactttcga ttttgaatgt ggttctacca
    32221 ccaacaacgt cgaacgccaa aaacaggcgg ctttctctga tactattttc gtggtcggta
    32281 cgcgcctgct gaccaaaaag ggcggcgata tcaaagattt tgccgacctg aaagacaaag
    32341 ccgtagtcgt cacttccggc actacctctg aagttttgct caacaaactg aatgaagagc
    32401 aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc cgcaccctgg
    32461 aaagcggtcg tgccgttgcc tttatgatgg atgatgccct gctggccggt gaacgtgcga
    32521 aagcgaagaa accagacaac tgggaaatcg tcggcaagcc gcagtctcag gaagcctacg
    32581 gttgcatgtt gcgtaaagac gatccgcagt tcaaaaagct gatggatgac accatcgctc
    32641 aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa aatccaattc
    32701 cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag
    32761 aaccgaatga caaggcactg aactaattac aagaaccagg ggcggaaatt ccagccctct
    32821 cgattgttac gtagcacgga cagactatac gcctgatggt cgttccccat cgggcctgaa
    32881 aaccgcaata cgctgggtaa caatcttcga gggtagcagt taacgctgct accctctttt
    32941 ttctggagta gatttatgtc tatagactgg aactggggta tttttttaca acaagccccg
    33001 ttcggcaaca ccacctatct tggttggatc tggagtggtt ttcaggtgac gatcgctttg
    33061 tcgatctgcg cctggattat cgctttcctc gtcggttcat ttttcggcat tttacgtacc
    33121 gttccaaacc gttttctctc cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg
    33181 ccgttgattg tgcaattctt tacctggtat ctggtgatcc cggagctgtt gccggagaaa
    33241 atcggcatgt ggtttaaggc cgagctggat cccaatattc agtttttcct ttcatccatg
    33301 ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag
    33361 tcgctcccgc gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct accgcaggct
    33421 taccgctatg tactgctgcc taatgcttat cgcgttatcg tcccgccgat gacctccgag
    33481 atgatgaacc tggtgaaaaa ctccgccatc gcctcgacta tcggtctggt ggatatggca
    33541 gcgcaggcgg gtaaattgct cgactactcg gctcacgcct gggagtcgtt taccgccatt
    33601 acgctggcgt atgtattgat taacgcggtt atcatgctgg tgatgacgct ggttgaacgt
    33661 aaagttcgcc tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca
    33721 ttgttccttc cctgccatat ctgctcgacg ggctggtgat cacgctgaaa atcaccgtca
    33781 cggcagtcgt gattggtatt ttgtggggca ccatgctggc ggtgatgcgc ttgtccagct
    33841 ttgcgcccgt agcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag
    33901 ttatggtttt gctgtggttt tacctgatcg tgccgggttt tctgcaaaac gtgctgggat
    33961 tatcgccaaa aaatgatatc cgtttaattt cggcgatggt ggcgttttcg atgtttgaag
    34021 cggcctacta ttcagagatt atccgtgctg gtattcaaag tatttctcgt ggtcagtcga
    34081 gcgccgcgct ggcgttggga atgactcact ggcagtcgat gaaactgatt attctgccgc
    34141 aggcgttccg cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata
    34201 cctcactggt gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg
    34261 agcgtgatgg tacgcaggtc gagatgatcc tgtttgccgg atttgtttat ttcgttatta
    34321 gccttagcgc gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct
    34381 gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt
    34441 gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt tccggaaaat caacgctgat
    34501 taaaaccgtc aacggcctcg aaccggtgca gcaaggtgaa atcaccgtcg atggtatcgt
    34561 ggttaacgac aagaaaaccg atctggcaaa gctgcgttcc cgagtcggga tggtgttcca
    34621 gcattttgag ttgttccctc atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa
    34681 agtgcttaaa cgcgataaag cgccggctcg tgaaaaagca ttgaaattgc tggagcgcgt
    34741 gggactttcc gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcagcagcg
    34801 tgtggcgatc gctcgcgcat tgtgtatgga tcctattgcg atgctgttcg acgaaccgac
    34861 gtcggcgctg gatccggaga tgatcaacga agtgctggac gtgatggtgg aactggcgaa
    34921 cgaaggaatg accatgatgg tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa
    34981 tcgggtgatc tttatggatg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt
    35041 cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact
    35101 ctggcgcgca tacccaagtg cgcgccatct cacgctttat cgtttcgccc ctcgtatctg
    35161 ttacatcctg gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact
    35221 gccaattctg ttagattgcg acccaggtca tgacgacgct atcgcaatag ttctcgccct
    35281 cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga
    35341 aaaaacctta cgcaatgttc ttcgtatgct gaccttgctc aatcgcaccg atattccggt
    35401 agcaggcggc gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg
    35461 cgaaagcggt ctcgacggcc cggcattacc ggaaccggcg ttcgcaccgc aaaactgtac
    35521 ggcggtagag ctaatggcga aaacgctgcg tgaaagtgcg gaacctgtca cgattgtgtc
    35581 taccggaccg caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa
    35641 aattgcccgt atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc
    35701 tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc
    35761 ggtggtgatg gccggtctgg atgttactca caaagcacaa atccacgttg aagacactga
    35821 gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt
    35881 cctcgaatat cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg
    35941 caccatcgcc tggctgttga aaccggagtt gtttaccact gttgagcgct gggttggcgt
    36001 ggaaacacag gggaaatata ctcagggtat gacggttgtt gattattatt atctgactgg
    36061 caataaaccg aatgccaccg taatggtcga tgttgatcgt cagggctttg ttgatttact
    36121 ggctgatcgt ctgaaatttt acgcttaaca cacgtcattg attaagatca ccacgcgtta
    36181 cctgggtggt cttaatctgt ttatctcttt ttctttcaag atataaatgc aatggataat
    36241 gcagaactcg ccattggtat cgatctcggt actaccaata gtttaattgc cgtctggaaa
    36301 gacggtgccg cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt
    36361 agcatggatg aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg
    36421 catccggata aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg
    36481 ttaggcagcg acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa
    36541 gaagatgccg aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct
    36601 tatttcagcg atgaacaacg caagcatacc cgtttagcag cggagttagc cgggttaaat
    36661 gcggtacgct taattaatga acccacagca gctgcgatgg catatggcct gcatacgcaa
    36721 caaaataccc gctcgctggt ttttgatctc ggtggcggca cgtttgacgt cacggtgctc
    36781 gaatacgcca cgccggtgat tgaagttcac gcctccgctg gcgataactt cctcggtggc
    36841 gaagatttta cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc
    36901 acgcttaacg agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc
    36961 aatcaatcgc cattgcacat tcgctggcag taccaggatg aaacgcggga gtgcgaattt
    37021 tacgagaacg aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt
    37081 gaacaggcgt tgcgcgatgc acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt
    37141 ggcggcgcgt cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta
    37201 ccgtatcaaa gttacgaccc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc
    37261 tgccgcttac gcagtgaaga tattgaagag gtgatcctca ctgatatttg cccttactcg
    37321 ttgggcgttg aagttaaccg ccagggcatt tccggcattt tctcgccaat tattgaacga
    37381 aacaccacag taccggtgtc gcgtgtagaa acttactcga ccatgcaccc ggaacaggat
    37441 tctattacgg ttaacgtcta tcagggagaa aaccacaaag ttaaaaataa cattctggtt
    37501 gaatccttcg atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt
    37561 agttatgata tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag
    37621 tccagagtga ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg
    37681 acgcggttat ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa
    37741 gccaaactgg aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg
    37801 gtgatcaccg attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc
    37861 aggcggcggg cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg
    37921 gcgtaaatgc tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg
    37981 attttgccga gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta
    38041 atgctctgtg gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca
    38101 tcgacatctt catcctccgg ctcgagtttt actttcgtca acagcaaccg ggtgataatg
    38161 gcctctaatg gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct
    38221 tgtttcggaa acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt
    38281 gaatcttctt ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg
    38341 aagaataacg actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt
    38401 tccgcccata acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct
    38461 tgcgcatcta ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg
    38521 caatgacgct tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactctcc
    38581 cagtatgcgc agagctcagc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca
    38641 cactggtaat accgggcatc ttcgctttct ggttcttccg tatagcgcgg cgattttttt
    38701 gataccgatt tgcatttcat ttgatactgc tgaaaaacgc cgatacgcaa cttatttgat
    38761 actaaatcgg cgcttttctt ttgagtttta gaatgagttt ttaagcgctc attcactctc
    38821 tttcaaacca gttttaactg tttgtggttt tgggtaaggc tggactttga attgctttgt
    38881 gttgaggcgt tttttggccc tcttgttcag aaaccggatg taacgaaact gtcggaactt
    38941 atgaacattg gctctgtcaa tattagccct cagatgttca cctcgttgtc cgcctcgttt
    39001 aatggcattc ctgcaaatct cgtgatacca ctcgccatca agttcataga atattgattc
    39061 atggctgcct acataatcaa aattgcttgc ctgatacacg acaccaagac agccacaacg
    39121 ctcatctgca aacgactgaa cccactgtac ctgcggataa agctgcctga ttagcttgag
    39181 cgcgtaactg attgcccttg attcggagtt tctcggcata cagtcatgca gccatagccg
    39241 gttaagctcc atatattctc ggttctgcgt acccgttacg acgcgtgcac cactgtttgg
    39301 gttaagggcg taaccccatt gcattacgcc aacaagttcc cgttctgaaa atatgcccag
    39361 atgaaggtag gagttattta cgaaacggtg gctgtaatgt ttattgacga tgaccagtct
    39421 ggccagccag caacttattg tctcaacccg caactcacgg gagccataac caacgatatt
    39481 gtcgttatag cgaataagtt caggcgtgct gatgattcgg gatgtgactt gctttctgtt
    39541 ccccacgaca ggatttcctt gtgtatgtgg ggtgctctgt ggcgctcgga gatgtgattt
    39601 gattgagggt tttacagcgc ggacatttta cttccagata actgaaactg gcccgtgcca
    39661 gtagcttatt gcagtgtcgg caccgtatgt ttcgatacat ggccgcataa cctcctgctt
    39721 tgttgttgat gcctttctca tgcaatcgat cgacaaaaac gatcgattcg atttgtttaa
    39781 ttgatacaac aaatagattg aaaagtcatc aacaatgatt caggtaggtg attatttact
    39841 taaagaaaac gaagagttag gtggtgaaaa cgatcataaa atgatcattt tattgacttt
    39901 ttatatagtg aaggtaggat cgcgcgtata tgtagtatgt ttcaaatcaa tgggatacta
    39961 catacataaa gagcgaccca gatagcgcga actattctgg gccgatttag acaattttgc
    40021 atcaatgctt tacttcgagc tcgtggcgta tcctatgata ttccccagtc tcctgtaaag
    40081 cagagatgca taaaaaggag atctttatca tggatgaaag agcctgtttg ccctgcctgt
    40141 aagactccct tacagctcat ctatcgccgt tctcgatctc aaaatggcaa gacatacaaa
    40201 cccaaaaatg cttcctgttt caggatgctg atatgtcctg cctgttctcc caggagggaa
    40261 aatacacaaa acgtgcatta acaaactcac ccggcaggga aacctgccgg tcggtattgt
    40321 tacatcatag taccgtcagt tccgggctga accggctctg gttgctctac gggttgagga
    40381 tcattttccg tgctgttttc agccggagaa cggccaacca ttcgggccag cacttcatca
    40441 acatatgcgt cgatttgtgc ctcaaaatcc ttgcgaacct gcgccttcag cacgcggttc
    40501 acttcttcag aataaagtgc ctgtttgaca agctcttcag taaccgtgcc tttaatctct
    40561 ggcatgaata ttctcctttt gcaggtatat tgccccgtta tcagagcggg gtcgggtcgt
    40621 ttggggatga cggtgcctgt gctgttttac tgagtttttc agctgccgca tcatcaattt
    40681 tacggcgaat ataatttctg atgaccttat aaccgccgct caccagatat aacgcactga
    40741 ccacagcgca gaaataaagc agcattgtat gaacgaatgt catataacct tacctttgat
    40801 attgactaca taattcgtta tcgggtaaaa acatcgaagc gactgataac cactccttcg
    40861 ggagtatttt tattttcctt tatgggttag gtagctcccg ccagccatat catgaccggc
    40921 gggatttttt tattctgtta ccgctttttt cacttccacc acaatgctgt caatcagcac
    40981 cgggtaagtt gcatttttgg tgatatccgt tacgcggagt ttgtccaccg ggaaagttcc
    41041 tactggtgac tgcgtcaggg taaacgcggc accatcctga ccatcaataa ccggaataac
    41101 ctgaaggctg ttatttcctg caaaacggaa ggccagcgta tgccattcat tatcaaatgc
    41161 accaaaggtt cccagtttct ggttatttgt tgccactttt gcattgtggt acatcacatt
    41221 caggtctttt gcgtctgtct ggatgtagaa cgatgccagc agattattcc ctgcatcccc
    41281 tgtcagggtg acaccctgcg gcagagagga aaccggccag taaagcgcca tgacatactg
    41341 gtttgccacc aggtcgccag gtactttaaa acgacaactg attaaaccac catttttcag
    41401 cagttcagca ccagtaccgg catcatgatc cagataccag gatgatttac ctgtttcctt
    41461 ggtcagtttc attgccttac cacctgtgcc tcctgcatca ctgataattt cagctttacc
    41521 gccagccggc tcccagccct gggctttcag atttccttct gactcactga cacggtaaga
    41581 cagcagtgtt gtggttactg cttcagtttc agaagatgga acatcggctt ttactgtggt
    41641 tcgccctgca tgagtcagaa cagccgtggc cagacggtca gaaataatac cccggcgcgc
    41701 ccaggcactg aaatggctgg cccggtccgc agatgtccag gtagcggcac tgttacgcca
    41761 cgttgagccg taataaccaa tggattcaat atccgggtct tctgccggat tgttggtggg
    41821 tacattcacg ccattttcat ctgtcattaa tggaacgaaa tgaatgtttt tatctgtttt
    41881 gtttttgtag ccgccataga ttgcctggta tgctggttca tttttctgtt tccagaaata
    41941 cgtggtatcg ccacaaatcc acggaacgcc agcagcactg ccaccggtac actgttccgc
    42001 ctgttcagcc aggtctgcgc ggaatttttc caccatcgcc agaaacagcg cagaatgctc
    42061 cgtcggcttc gcatcaatat caaactcgcc ctgcatccac accacagcaa gcaggcggtt
    42121 tttcgggttc ttcgccagtg ccgcttttgt ccggctgatt aagtccttat acagcggctt
    42181 atccacaccc cagcgggctg agtcctctga agcacccgtg ctgtcactgt acgtgccgtc
    42241 agcacccgcc gtgaatgcag aaccaccacg gcagcaggga accagcagaa tccccgcatc
    42301 tgccggaata aacggcagca gttttttggc aatgtgaagc ccctgaccca cggtgccata
    42361 ttgcccttta gccacatccg ctttcgggtg attttttcca ctcatatcct gcacatcatg
    42421 cagacagtgg tcggccagaa tgatgtcgtt atactgacag gctttacctc ccggcgtgac
    42481 tgtactgcga cgggctaact gcataatacg cggttcgggg cggtcatatg tctccggcag
    42541 tggcagacct tcaccgtaag ccataccgtt tgactgacca gcaagaacca ccacgtaata
    42601 ccaggaaggg gagctggaag cagtttcatt tcctgacgga cgggtatgtg tttcagcatt
    42661 atcccagtct gacggttcag cagtaccggc aacatcagca cttaacggaa tgaccgcacc
    42721 aatacccgct ccggtaattt ccacactcgg atcaccggat actggcctta cccacaggca
    42781 atcccgtgta ttaaaactga tgagacagtc agcaaatgtc attcctcctt ttgtttcggc
    42841 cggaggaaag ggagcctcaa gaaatgccgc tgtaccaccg gaaactttaa cagtaaagtt
    42901 tccgggcatt gatgatagtt gaacccattt ggacgtcata ataatcagcc tgttcctgaa
    42961 taaactgaat tatcatatcc acagaccgat aaacgccatt ttgcgttaat tcagtaaagg
    43021 cccaacaaag agagcgtctt atttttccga aacgctcgca ggataataac gataaagggt
    43081 tgaaagaccg atatcaaaaa taagtgccac ctgtcttcga ctgacacccc cggccagtaa
    43141 acgcccgacc tgctcgtatt gttcctgcgt gagacggcgg ggtctgccgc cggttcgtcc
    43201 cctttcacgg gcagctgcaa gaccagcccg tgtccgttcg acaattaatt cacgctccat
    43261 ttctgccagc gcacccatta tgtgaaaaaa gaagcgcccc atgggggttg acgtatcaat
    43321 gttgtcagta agactacgaa aatttatccc ctgttgctgt aattcatctg tcagcgcaac
    43381 aaggtggcgc atactgcgcc ccagcctgtc cagtttccag accatcagcg tgtcaccggg
    43441 ttttagtttc ttcagtgccc gcttcagtcc cggccgctgt gtcgttttac cactcatttt
    43501 gtcttcaaaa atctgctcac atcctgcaca ttccagcgca tttctttgta aaccggtgtt
    43561 ctggtcattt gttgatacgc gtatatagcc aatcagcatg tatttacctt ccttaaaaag
    43621 aggtggaaaa gccttttaaa gtagatatac ggcatccctg aaacctcggt ttacgggata
    43681 cggtaaacag ggccgcgaat gccctgccat caaacggtac ggctgttgca gcaagcaggc
    43741 tggcaaatgc ccgcagaatc aatggtgtcg cctttgacgg cacgcaggat ataaacatta
    43801 cgtcaggaat gccggaatca acggccaatc agcgttatgt tacaggggta agactgggag
    43861 cgcaggcatt aagcggcgga ctggaatata attactccct gtcatcagga aatgtgatta
    43921 ccggttttaa gactgacgga gactgggaaa tgcgtggtgg tgatgaccgt gtttattaca
    43981 gacagataca atattgtatt aatgggcatt gggtatccgc tgcaagtatt taattaacgg
    44041 gggcatagca cacagcagcc agcatctgaa atatgcgtta tttcagacaa gcattgcctg
    44101 tatatggcga tagtattatt cctgttttta tcattgattt acatggtgat gtttatgcaa
    44161 ctgattaatg tcaaacgata ttacccgaaa gacaaaccct atggtgagga tgttcagtat
    44221 ttccagagtg aggacggaag ggatttttat gaatctatcc cgctttttac aaagaaatac
    44281 aaactctgta tttcgcctgt aacgggtatt atctgctctg ttgctgaaga tgtctctgct
    44341 ttatatccgg caggttttac tgttgttgaa gtggacgaac tgccggaagg tgtgaatatt
    44401 gacggtaact ggcagttttc tgatggcctg atatcaaagg tgccggtaaa ctggaagacg
    44461 gttgctgaaa agcgtcgctc atcgctgctc caggaagcca acgaaaccgt ggatgactgg
    44521 aaaacagaat taaaactgga catgattagc gatgaaaata aattacagct gacgcgctgg
    44581 atggcgtata tccgacagct taaagaaatg cactttaatg atattgccag tgaggggcat
    44641 taccaggcga ttccctggcc ggaaaaaccg gaataattca gagggtgcat tcagcaccct
    44701 tttctgtggt ttacactgaa cttaccgttc tccaggtatt atgaatataa atctgaaccg
    44761 ggcgataata gaccccaccg atattgtccg cagaccggga gccggtttcc tgcacgttaa
    44821 taccggaaag catacaaccg gaaggtgcgc tgtaattcca tgacatttca ttgcctccgg
    44881 gtgaatgata gctctgtgct ccaagctgaa cattctgtac ataccggcca tctgcggttg
    44941 actgcgtcat tcctgacgtg atgttgatat ccagcgttcc gtcaaaagga acaccattaa
    45001 tatttcttgg ggtggcgagt ctgtttgctg ccacggcggt tccagccgat ggcagggcat
    45061 cagcagcctt atttaccgtt tcccgtaaac cgaggttatc gataaaggtt cctttgtcct
    45121 ggatatccgc gccattctgg tccctgcgca ggtaacggtt atcaccttcg ctcctgctgt
    45181 aaacatccag attattacgg gcggtttccg cattctccag gtcggcaagg ttctgatcct
    45241 tcgccagggc attaacgtca gcgggaccaa gactgttttt tgtggcaagc gtccccagac
    45301 caagataacc acgggccaga cgctgggctt ccgcaccggc atcagcaatt tctttcaggt
    45361 tgtttttccg ggaaaaggca ttcgcagcga taatggcttt aatggacagc gtaagctggt
    45421 ttaactgcgc cttatccggc tgaatactgg cttcagccag aatattcagc agttcggcct
    45481 ggagaatatt cagccagtcc tgaccaatcc agctgatacc tttttgtccg tcaccttcgg
    45541 taaaccaggt ggtggtatta ctctgcgccg gagccagttc aggcatattc gccacgccgg
    45601 aattattatc aacatggaac attaaatatt ctcctctttg tgactgtttg catacacgta
    45661 aataaacgtc tgccatgcag gcttataacg gttcagaata cattccagcg caccaccctc
    45721 ataaatacgc agcggggtga gaatatcatc cagcacattc atattccggt agccggtttc
    45781 actgtttatc gtgacaatac tgacccactg tgaatccggt gatggctgaa tatctatttc
    45841 ataaccaaac tctgccgcaa accggatata aaattcacgg ttaagagagg gtttcatccg
    45901 gtatttattc cctgcataac gctgacgttc ctgaatggtt gcgccggtca tatcacattc
    45961 cggtaacccc agataacgct cccagtcttc cagcagcaga cgggatgaat caggaaaacg
    46021 ttctgccagc atctggttac cggtccagga aacacgctcc gtggaatgac tgaggcccag
    46081 gcacaaagcc gcaagaacgg atgacggccg cttatcccat gccagcccgt caggcagcaa
    46141 ctgcaacagg gcgcgctgat gcggggtcag ggttacagcc atgtgatttc ccccacggtc
    46201 agcaactctg tatctccggc catcacggac ttcagcggac tgcgaacttc aaaatcctcc
    46261 agtcctttca cccctgcaac agcccgccag aatgatgaag gcaatacaag tccacccggc
    46321 cgcgattcgt tataaagcag gtccgttaat gcctgtttta cggcagcctg attttccggc
    46381 gttttgggta caatcctgat ctcaaacgcc accggcttat tggtcagctt aaacacgctg
    46441 attgtcggcc caagtggctg accaacgggc tggcccgtcg ccggatcatc atgactgcgg
    46501 atataatccg ccacccgctt cacatcacct tctccgggga aaatgtcagg gttattatcc
    46561 tgaacaaaag tcacaccaac actacccgcc tcaggccatt caggcttaca ccatgcccgc
    46621 gtcacgcccg gtacttcccg tgcccagcgt tcaaaatcat actgtgttcc ccctgatggc
    46681 gggttctgta cccggaatac cagcctggac agcaactccg gcaccgtttc cacatccgca
    46741 ccaccggtga gtcccgtgcc ggtaaccgtg gctgtctggt tgatgcccgc ctgtggcgtg
    46801 ataaaggaca ggcgggttcc ggtcggggta tttccggcgc gccccgcact ttccgcctcc
    46861 acattaacat tcagtgttcc ggcttttccg gtcgcagagc cggtgatgcg gtacacaaca
    46921 ccatcgcttc gctgaaggag tacgccttcc gtgatccctg catccgtggt cagcatcagt
    46981 tgtaccggtc cgtcagcgcg ggaagccggt ttacggatga caccccagaa tgcgcagtgt
    47041 ttcaggagtt cggcttcatc agcttctgtc ggaattatct gccgggagat ccaggccaga
    47101 tgttcatgct cctgtgctga cagacccgcc agtgcatacg caatggcatt cagggtggtt
    47161 tcattcacgc ccggctgcga accgggcagg cgctggctga tatcctgttg tgtctgtgtg
    47221 atcagttgcg ccagtggtaa gggctgataa ggcatcattc ccccttcata tcagcataaa
    47281 aaatcatcgg atgtctgctt ccgtccggca gggtgatttt cacggaaagc gaaaggcgat
    47341 catgccccac acgcatggca tgacatacca cggatgtggc agcaccgctt ttatgcagcc
    47401 acgccagcgc ttcatcagca taggcctgtg cacggctgac cacggaggac agcgtctttt
    47461 cacggcttaa cagccagaga cgggagccaa tggggcgctc ccggaaactg tctccccacc
    47521 atccccgacg gtcacgggtg ccgtcaggga tttcatcgga atccagcgcc cgcctgtccg
    47581 taaacagtga aataatgacc gcagtcagca ggctgttatc cgtcagaaga tctgcgccgt
    47641 tcagttgcag ggaaccacaa ccctgatccc agacgacagc aatatcagcc attctctggt
    47701 cctcccgttt tgctgccttc accgttatcg tgatgcacat gacctgaata ggtgatcccg
    47761 gcaataaccg cttcggacat cgtgaacgcc ccctgtgatc ttccggtgcc gtcaatggac
    47821 acattgcctt tgacatgcag gttcttatcc actgtgatat taccggtaaa gtgagcctcc
    47881 ggtgtatcca cctgaacacc ttcatcggcg taaatctcca gtgtcttaca ggtcacaatg
    47941 atccggccgt tctttgtcag acgtatacgg tgcccctcat ggtgatacac gccggtatct
    48001 ccggcggtaa gtccggtcgg gcgactgcgg cggtcttcca ccacaagcac cacggcctga
    48061 tcccgttgtc cgccagcaca ggccagaaaa acttccgcac caggcagggg gacgctgatt
    48121 tgcccgtact gctgggggcg ttcaacgtca tcaaaggttt caccatccat accggtgatt
    48181 tgcacattct ggattttcag cgtgtccgtt gtgccggtca gtacaccacg cccgaacagc
    48241 agacgaacac cccgcatcac gggggccaga aggcggctga tgacttcgtc attcatcatt
    48301 gaatttaatc ccctgttttt tcatctgtgc ccgcacgaag gcgtccacgt cacccgcatt
    48361 tccgcttccg ctgtttcccg gctctgccgg aacaataaag ccgtcacggg gtgccagaat
    48421 cagacgggtg gtttcgccgt gatttgcatc cagcgaaaac tccacctgac agacaagaag
    48481 atcacgctct tccactccgg tgcggggagc cgtgacagcc gtcagcaggt tgatatccca
    48541 caactggccg ttctcccgaa accagtgccg cacggtggct gtcaggcgca cagagcgggc
    48601 aaccgcccgg cgcatttccc ggacagccct ctggcgtgca ccgtctgcat caacagcatg
    48661 atccgccagc acaattttcg gtctgtaacg gtggatggca ctgtctgtca ccgtaccaac
    48721 tggtgcggcc agcgttccgg ctgtcagtgc atccccggca tgaccaccac cacgcccgtg
    48781 cccccgtaca cggtattcac tgtggcgcag acggtgatcc acgttgtgat ccaaatccag
    48841 cagattttcg cccagcgtca gcgtgtcgcc acgctgactg ccggcctgag tgaaaaccag
    48901 ttcaccggcg gcattactgg ttaccaggac gcctctgtgt ctcgcggccc gtgtcagcgc
    48961 atctgccacg gtttctgaat tttccagtgt gaaggtggaa aagggccggg ctgccgttgc
    49021 atcatttacc tgccagcgca ccgtgacccc gaacggagca cacaaatctg aagcaatgtg
    49081 ctccagcgtg cggttcctcc actggctgcc cggatgaatg gctgaacagt ccaccaggtc
    49141 accggtttta tcccgtccgc tgagcgtgat ctgaaaacgc gtggcgctga tgcgctgccg
    49201 gacctgatcc agccagccag tgatcacagg ctgaccgtca atgcgcaatt ccagagactg
    49261 accggcacgg acggatgacg gaaggcgtac accgggcatc attaccccca gttcaaagga
    49321 gcccgccaga tgttcaagag agcgacgaac actcaccgtc agccagccgg aaaaaatctc
    49381 accgcccaga tacagttcaa ccctgctgct cactaatcac ctccactgaa tggcctccgg
    49441 gaatgaacag cggatcaaca ataccgttac ggcgcacaaa acgttgccag ccggtactgt
    49501 tcccggtggc acggtacagg gtcaccagtg caggctcggt ggtacgtaca gtcactactg
    49561 ttgcaccggg tagctgaact ccccgtgtat tcagatcctc tgtcagtgcc agtctggcat
    49621 cacgaagcgt cagcgccgtg gcggtatacc cctgcccgga cagtgtcatc acctgccgtt
    49681 cgagcgcttc gcttaagtca cggttaatgc gctgaagatc aactgaactt tccagccaga
    49741 caggccaggt acggtgggct gcgtcactgt ccggcagggt ttcctgactg agaacctgat
    49801 ccagcagttt tcccacggtc tgtgcctggg ccaccacggc agcactctgc attaccgccc
    49861 gtatcagacg aatattcttt tgtgctgccg gtgtcagacc gatgagagtc tgcggattat
    49921 cctgctcatc cagcacgtaa cgcagggaag ataatgtcct gtacagttgc ggcattccct
    49981 gagagacggc ggatgtcgtc cggccgatac tgccaccggg ccgggtattc aggcctcctg
    50041 aagacagtga cggcagcgat ggcagggtga tcagggcact gaaaagcccc gcaaaagatg
    50101 aggccatgcg ggaaggtgct gtcagtaatg ccgtggcatt ccctttcagc gccgtgaacg
    50161 tggcagtaaa ggcactgata tcctgtacaa tccccatgcc ggaaacggca ttttccaggg
    50221 cggacacctt atcactgatg gtttcggtca ttgcctggac atcgtgcatt ccgtcagaga
    50281 tcaccgtcca gcccgcttcc agggtttcaa aaacctgccc gagcgcggtg gtactcttct
    50341 gctccagaat ggcggccgtg tcctgtgtga cagcaggtgc ggtatcatca cttaccggcg
    50401 tgacattaat ggtgaactca accacgccct gttcagcggc gttataacga ctttcaaagc
    50461 tgtttatcag cacatttaac gtgccgtaat ccgggtgaag cagctccccg gcaccgggag
    50521 cacgaagggc atcacgcaga cgatttcgct gtgtctgaac atcatccccc atcacaagta
    50581 cactgaacgt aaattcagtc agtttaggtc ccagatcgtc tgccccaccg gtttcccgta
    50641 acggatattc ccgacggaca atgtttcgcc caccacgttc gcgctgctca cgccagacga
    50701 ggaacggcac accacggaac gcaccacgac cgtcaccaga ggccataatt ccctccgttc
    50761 cagacattca catccagtcc gtgaccggac gtgtcatcaa tatcaatact gcgagcctgc
    50821 cacccttccg gggcaaccag ctccacacgg gcagaggctt tttgtaccgc ctgtccactg
    50881 ctttcatcat cactgccccg taatttctgc caggcttcag tcagccatcc ccccagatag
    50941 ttaccgagat agctgcccac cgtagaacca atggcaatcc ccacaggtcc ggctgccgcc
    51001 cccagcgcac caccggcaat actgcctgca agagagccga ccgcttctcc cttgtctgtg
    51061 gcactggcgc tgccatccag cagaaccggg gctgccatcg cgccagccca cagggcacct
    51121 ccacccagcc ggccggccag tccaccggca ctgcggaaaa agccggagaa acgccccagc
    51181 cccatgcggc taccgacaga cgaaagtaac ccaccggcgc gc
//
LOCUS       DAZFFI010000023        49686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFFI010000023 DAZFFI010000000
VERSION     DAZFFI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.023"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..699
                     /gene="comR"
                     /locus_tag="ACHJ12_001630"
     CDS             67..699
                     /gene="comR"
                     /locus_tag="ACHJ12_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4989429.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(761..1300)
                     /gene="ycfJ"
                     /locus_tag="ACHJ12_001631"
     CDS             complement(761..1300)
                     /gene="ycfJ"
                     /locus_tag="ACHJ12_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4989430.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1527..2831)
                     /gene="ndh"
                     /locus_tag="ACHJ12_001632"
     CDS             complement(1527..2831)
                     /gene="ndh"
                     /locus_tag="ACHJ12_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4989431.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHJ12_001633"
     CDS             complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHJ12_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4989432.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHJ12_001634"
     CDS             complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHJ12_001634"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4989433.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHJ12_001635"
     CDS             complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHJ12_001635"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4989434.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHJ12_001636"
     CDS             complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHJ12_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4989435.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6292..6669)
                     /gene="ycfL"
                     /locus_tag="ACHJ12_001637"
     CDS             complement(6292..6669)
                     /gene="ycfL"
                     /locus_tag="ACHJ12_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4989436.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHJ12_001638"
     CDS             complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHJ12_001638"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4989437.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHJ12_001639"
     CDS             7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHJ12_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4989438.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHJ12_001640"
     CDS             complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHJ12_001640"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4989439.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11342..12139)
                     /gene="ycfH"
                     /locus_tag="ACHJ12_001641"
     CDS             complement(11342..12139)
                     /gene="ycfH"
                     /locus_tag="ACHJ12_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4989440.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHJ12_001642"
     CDS             complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHJ12_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4989441.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHJ12_001643"
     CDS             complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHJ12_001643"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4989442.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHJ12_001644"
     CDS             complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHJ12_001644"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4989443.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHJ12_001645"
     CDS             complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHJ12_001645"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4989444.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHJ12_001646"
     CDS             complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHJ12_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4989445.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHJ12_001647"
     CDS             complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHJ12_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4989446.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHJ12_001648"
     CDS             complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHJ12_001648"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4989447.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHJ12_001649"
     CDS             complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHJ12_001649"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4989448.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHJ12_001650"
     CDS             complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHJ12_001650"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4989449.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHJ12_001651"
     CDS             complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHJ12_001651"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4989450.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHJ12_001652"
     CDS             complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHJ12_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4989451.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHJ12_001653"
     CDS             complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHJ12_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4989452.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22321..22905
                     /gene="yceF"
                     /locus_tag="ACHJ12_001654"
     CDS             22321..22905
                     /gene="yceF"
                     /locus_tag="ACHJ12_001654"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4989453.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHJ12_001655"
     CDS             complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHJ12_001655"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4989454.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24549..27734
                     /gene="rne"
                     /locus_tag="ACHJ12_001656"
     CDS             24549..27734
                     /gene="rne"
                     /locus_tag="ACHJ12_001656"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4989455.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27930..28883)
                     /gene="flgL"
                     /locus_tag="ACHJ12_001657"
     CDS             complement(27930..28883)
                     /gene="flgL"
                     /locus_tag="ACHJ12_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4989456.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28895..30538)
                     /gene="flgK"
                     /locus_tag="ACHJ12_001658"
     CDS             complement(28895..30538)
                     /gene="flgK"
                     /locus_tag="ACHJ12_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4989457.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30604..31545)
                     /gene="flgJ"
                     /locus_tag="ACHJ12_001659"
     CDS             complement(30604..31545)
                     /gene="flgJ"
                     /locus_tag="ACHJ12_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4989458.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31545..32642)
                     /gene="flgI"
                     /locus_tag="ACHJ12_001660"
     CDS             complement(31545..32642)
                     /gene="flgI"
                     /locus_tag="ACHJ12_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4989459.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32654..33352)
                     /gene="flgH"
                     /locus_tag="ACHJ12_001661"
     CDS             complement(32654..33352)
                     /gene="flgH"
                     /locus_tag="ACHJ12_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4989460.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33405..34187)
                     /gene="flgG"
                     /locus_tag="ACHJ12_001662"
     CDS             complement(33405..34187)
                     /gene="flgG"
                     /locus_tag="ACHJ12_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4989461.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAASVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34325..35080)
                     /gene="flgF"
                     /locus_tag="ACHJ12_001663"
     CDS             complement(34325..35080)
                     /gene="flgF"
                     /locus_tag="ACHJ12_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4989462.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35100..36305)
                     /gene="flgE"
                     /locus_tag="ACHJ12_001664"
     CDS             complement(35100..36305)
                     /gene="flgE"
                     /locus_tag="ACHJ12_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4989463.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36330..37025)
                     /gene="flgD"
                     /locus_tag="ACHJ12_001665"
     CDS             complement(36330..37025)
                     /gene="flgD"
                     /locus_tag="ACHJ12_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4989464.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37037..37441)
                     /gene="flgC"
                     /locus_tag="ACHJ12_001666"
     CDS             complement(37037..37441)
                     /gene="flgC"
                     /locus_tag="ACHJ12_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4989465.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37445..37861)
                     /gene="flgB"
                     /locus_tag="ACHJ12_001667"
     CDS             complement(37445..37861)
                     /gene="flgB"
                     /locus_tag="ACHJ12_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4989466.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38016..38675
                     /gene="flgA"
                     /locus_tag="ACHJ12_001668"
     CDS             38016..38675
                     /gene="flgA"
                     /locus_tag="ACHJ12_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4989467.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38751..39044
                     /gene="flgM"
                     /locus_tag="ACHJ12_001669"
     CDS             38751..39044
                     /gene="flgM"
                     /locus_tag="ACHJ12_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4989468.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39049..39465
                     /gene="flgN"
                     /locus_tag="ACHJ12_001670"
     CDS             39049..39465
                     /gene="flgN"
                     /locus_tag="ACHJ12_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4989469.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     HNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39505..41040)
                     /gene="murJ"
                     /locus_tag="ACHJ12_001671"
     CDS             complement(39505..41040)
                     /gene="murJ"
                     /locus_tag="ACHJ12_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4989470.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41150..42073)
                     /gene="yceM"
                     /locus_tag="ACHJ12_001672"
     CDS             complement(41150..42073)
                     /gene="yceM"
                     /locus_tag="ACHJ12_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4989471.1"
                     /translation="MKKLRIGVVGLGDIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42075..42722)
                     /gene="yceH"
                     /locus_tag="ACHJ12_001673"
     CDS             complement(42075..42722)
                     /gene="yceH"
                     /locus_tag="ACHJ12_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4989472.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42733..43317)
                     /gene="rimJ"
                     /locus_tag="ACHJ12_001674"
     CDS             complement(42733..43317)
                     /gene="rimJ"
                     /locus_tag="ACHJ12_001674"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4989473.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43553..44761
                     /gene="mdtH"
                     /locus_tag="ACHJ12_001675"
     CDS             43553..44761
                     /gene="mdtH"
                     /locus_tag="ACHJ12_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4989474.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGCSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44825..45472
                     /gene="grxB"
                     /locus_tag="ACHJ12_001676"
     CDS             44825..45472
                     /gene="grxB"
                     /locus_tag="ACHJ12_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4989475.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45606..46166
                     /gene="yceB"
                     /locus_tag="ACHJ12_001677"
     CDS             45606..46166
                     /gene="yceB"
                     /locus_tag="ACHJ12_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4989476.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46272..47318
                     /gene="pyrC"
                     /locus_tag="ACHJ12_001678"
     CDS             46272..47318
                     /gene="pyrC"
                     /locus_tag="ACHJ12_001678"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4989477.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47392..47637
                     /gene="dinI"
                     /locus_tag="ACHJ12_001679"
     CDS             47392..47637
                     /gene="dinI"
                     /locus_tag="ACHJ12_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4989478.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47927..48181
                     /gene="bssS"
                     /locus_tag="ACHJ12_001680"
     CDS             47927..48181
                     /gene="bssS"
                     /locus_tag="ACHJ12_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4989479.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48296..49414
                     /gene="solA"
                     /locus_tag="ACHJ12_001681"
     CDS             48296..49414
                     /gene="solA"
                     /locus_tag="ACHJ12_001681"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4989480.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49462..49575
                     /locus_tag="ACHJ12_001682"
     CDS             49462..49575
                     /locus_tag="ACHJ12_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4989481.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12062 a        13386 c        12314 g        11924 t
ORIGIN      
        1 ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac caggtacaac
       61 ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg
      121 ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcaaca cggttatgaa
      181 gcgacttctc ttgcggacct cgtcgaagcg accggagcca aagcgcccac gctatacgcg
      241 gaatttacca acaaagaggg gttatttcgt gccgttctcg accgctatat cgatcgtttt
      301 gccgctaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct
      361 gactattttg ctgccatcgc caactgcttt accagcaaag acaccccggc aggctgcttc
      421 atgatcaaca actgcaccac cctctcccca gattcaggag atatcgccaa tacgttgaaa
      481 tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc
      541 ggggaaatcc cgacccactg tgacgtgaca catctggcag aattccttaa ttgtattatt
      601 caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg aaaaactaat gcagattgcc
      661 ggaacgactt tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag
      721 cctgagcgaa tgaggggcca aattgcagag tacagccttg ttatactttg ttattcaaaa
      781 tcagttgccc attactatct agcgggatct gcgtacccgg atcgcggtcc atgcggattt
      841 tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt tctgacttgt
      901 catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc
      961 cctggatctg gttaccggca tatccaccac ccagcgcccc cacaacagtg gcgacatctt
     1021 taccgcgacc accgccaaac tgatgcccta tcacgccgcc agcaacagcg ccgagcaccg
     1081 acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac
     1141 actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca acctgagcgt
     1201 attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgccc agcgcagctg
     1261 cgacaccaat cccgataccc gccaacattg atttattcac gggaacacct ccttctttgc
     1321 agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag atgaaaagcg
     1381 cgggaatggc tggaaatact taatgattcg gaggagtctg aaggaatgcc tgatgcgacg
     1441 cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga tgaggcgttt
     1501 atgccacatc cgccagtgta cgtcgattaa tgcaacttca aacgcggacg gataacgcgg
     1561 ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag cgcaatctga
     1621 tgcattcggt atagcgagat atatacaaag cgcgcaattc gtccttcaat catcattgag
     1681 ccgcgcgtca ggttacccat caggctacca acggtggaga agttcgacag cgataccagc
     1741 gaaccatgat ctttatactg ataatttttc agcggcttac cattcatctg cgccagaatg
     1801 ttgttcattg cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg aacaaagccc
     1861 ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgggtg
     1921 gtttgcagcg tcggttccac caccagctgg ttgatacggt tagtttcaag accaccgata
     1981 tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc agcctcaata
     2041 tattcgccat ctttagtgtg caggccgcct ttatcagcac tggtgaccat ggtttgcgtc
     2101 agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg cagcagagat acgtggcggt
     2161 aatgcaggca aaatacgttc tcccgcttct accagcgtta cgttcagggc ttcgttggtc
     2221 aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt tgtgcaattc agcggagagt
     2281 tctacacccg tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt tgcgcccagg
     2341 ttggcggagt atttcaggaa gagattcagc atctcctggt gaaagcgacg cgcctggtgc
     2401 gggttatcga ggaaaatgca gttctctttg acacctggcg tattgaaatc gttagaggtg
     2461 ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac cagcagttca
     2521 cctttctcat cgcgcagttc tgcaatagtg attgttttcg cttcacgatc aatatcaatg
     2581 acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata gctcaacgca
     2641 tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt ccacagatgg
     2701 ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag cttatgcccc
     2761 agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat ctttttcaat
     2821 ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat ggttattaac
     2881 atagctctta attaacaaca ggttacagca atgaaaatgt tcttcgctat tgagaataac
     2941 atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg ctgaaaagat
     3001 aggcgacttt accgggtgag atcaaaaaac agagtttatt gtttattatt tgagcaagtc
     3061 cggaagggcg atatagggtg aagcgtatat cgagggcgtt tggtcgtcct gataagaggc
     3121 gcatcaggca ttgatgctcg aatgtcggat gcggcgtgaa cgccttatcc gacccacaga
     3181 atccggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg
     3241 gttttgaacg ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc
     3301 tcttcgtccc agacaatctc gtaataatga tgcagctctt cagatgtccg ctggctgtta
     3361 agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcacggaag
     3421 ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc
     3481 atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa
     3541 ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg
     3601 acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga
     3661 tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact
     3721 ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc
     3781 tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt
     3841 cagacgggtg ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga
     3901 acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac
     3961 gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga
     4021 tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc
     4081 agagaaaatc acgccgtcaa aacccagttc ctgacgcaaa acggttttca gccagtaggg
     4141 agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc
     4201 gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc
     4261 cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg
     4321 tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg
     4381 gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc
     4441 gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat
     4501 ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcctga
     4561 cagcgcagcg aatgattgcg ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac
     4621 gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgaagctg cgcggatctg
     4681 gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc
     4741 tcccaccagc ggatgcgcaa gtatttcacg ctcttccgcg tccagttcgt aacctttgac
     4801 atccaacatt actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg
     4861 tgtcatcggc cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc
     4921 accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg
     4981 ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat
     5041 tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt
     5101 cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca
     5161 atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca
     5221 gccaacccac tgtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaacggca
     5281 acagcgttat tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca
     5341 gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc
     5401 agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc
     5461 gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa
     5521 aacgctggcc ctgatgttca atgagaaaac tcccgccact aagcccacta ttaaacgtcg
     5581 tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcatg atgggattat
     5641 tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc
     5701 tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga
     5761 gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc
     5821 gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc
     5881 ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg
     5941 ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc
     6001 ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc
     6061 ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc
     6121 ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt
     6181 ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc
     6241 agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata
     6301 tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc
     6361 cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct
     6421 ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag
     6481 cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat
     6541 gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc
     6601 cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc
     6661 ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac
     6721 aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga
     6781 cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg
     6841 cgtcccagcg cctgctcatg ctcagctgag acgtcgttca cagtcggaat gaggatattc
     6901 ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga
     6961 tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt
     7021 tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg
     7081 ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga
     7141 tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct
     7201 gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgtatat
     7261 ttctcatttg catttacaaa caaaattatt cgcacactaa atataatcgc tcatcaccct
     7321 cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat
     7381 ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag
     7441 cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg
     7501 ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct
     7561 ctgcgggtac caaaatgcag atgactcaac gtgatattcc tcagtcggtc actattgtta
     7621 gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc
     7681 tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc
     7741 agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg
     7801 gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag
     7861 gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga
     7921 ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt
     7981 atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg
     8041 gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct
     8101 ccggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc
     8161 agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca
     8221 gcagcaacag ttacgatcgc gcacgcagta ccgcacctga ctgggcgtac aacgataaag
     8281 agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacgc
     8341 tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg
     8401 taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt
     8461 atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg
     8521 ctgacggtag ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca
     8581 gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca
     8641 gtttctacaa ctttaatggt aatttcccac aaaccgactg gtcaccacag agcctggcgc
     8701 aggacgatac cacacatatg aaatcgttat atgctgccac tcgtgtcacc cttgccgatc
     8761 cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca
     8821 gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact
     8881 ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag
     8941 gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga
     9001 tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc
     9061 agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa gcagtggatg
     9121 ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc
     9181 tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta
     9241 atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt
     9301 tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt
     9361 atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc
     9421 aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg
     9481 ataccaacgt ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca
     9541 cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggctgcct tagtctcccc
     9601 aacgtcttac agattagtgg ttacggatgt actcatccat ctcggttttc aggttgtcgg
     9661 atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca
     9721 gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg
     9781 taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg
     9841 gtgccatttc gctggtacct gtcgcttttg catcttcagt cgcgtcttca cgacccggcg
     9901 ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac
     9961 cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga acgatgaagt
    10021 cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga
    10081 atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg
    10141 agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa
    10201 taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag
    10261 ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac
    10321 ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac
    10381 caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggt accaggcaac
    10441 gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag
    10501 agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa
    10561 agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat
    10621 actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga
    10681 taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta
    10741 aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa
    10801 ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa
    10861 aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa
    10921 cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca
    10981 gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct
    11041 taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca
    11101 aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt
    11161 aaaataattc agtgccaatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag
    11221 aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc
    11281 aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca
    11341 ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg
    11401 tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa
    11461 cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag
    11521 gtgagtcagt ttccaccagt aatcgatcca gggggacata acgcgcagca tcgcgcagtt
    11581 gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta
    11641 atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca
    11701 ctttttcttc gcgcagaatt gccagcgtat cggcacgggc gtcacgggta tggacgataa
    11761 ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgta
    11821 ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct
    11881 cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat
    11941 gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat
    12001 aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg
    12061 ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt
    12121 gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc
    12181 ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg
    12241 cctgtaacag acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg
    12301 cgcgagggag aaagatggtt tgccagttcg gcgaccaggc ccggcacatc aacattagtc
    12361 acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt
    12421 aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat
    12481 ggcacgctat atgccaacgc ctgacacaat gtttcacgag cctgccagtt atctccctga
    12541 aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca
    12601 tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc
    12661 gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg
    12721 gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta
    12781 gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg
    12841 cgtgcgtgct cattcagctt ttcggtgacc tcacgtaccg catcaatgcc cagcatattt
    12901 ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga
    12961 catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg
    13021 ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca
    13081 tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat
    13141 ggataccatc tcatgcgtcc aactccttaa cccagtgggt cacggtagtg cggattgcat
    13201 ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag
    13261 ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac
    13321 gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat
    13381 agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat
    13441 gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc
    13501 caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc
    13561 gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt
    13621 tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag
    13681 tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg
    13741 tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc
    13801 ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac
    13861 gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca
    13921 gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg
    13981 gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa
    14041 cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa
    14101 cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg
    14161 ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc
    14221 ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg
    14281 acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac
    14341 tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg
    14401 tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct
    14461 accagtcgca gcgggaactg tgcttcttta ccgctttcca acaatttcag catctcgcgc
    14521 acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg
    14581 atacgcagca gccattgaaa aacccgtggg cgattgatga tcttatcagc ataaagctgt
    14641 tcaccgagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata
    14701 agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc
    14761 agtaccacca gcaataacaa gattatcaat aacacttttt tcatgactaa ttcgggcgct
    14821 cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg
    14881 cacgtacggg catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg
    14941 cctcttccag agaagcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga
    15001 tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc gtataaacta
    15061 cgttgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt
    15121 caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcgaatc aatacttgct
    15181 caagacgatt gagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc
    15241 ttagcgccaa cgtcatcccc tcgttacgca aacggtcgta atgtgcagga taagccgtaa
    15301 cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac
    15361 cgcgactgat cacgactttc agtacaccat tttgctgttc tgctgccagc gttttcatct
    15421 cttgttcaag ctgtggccag aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta
    15481 gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa
    15541 aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttataac
    15601 cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac
    15661 tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttataagg
    15721 gtggaaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc
    15781 gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa
    15841 atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc
    15901 gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta acagatgacc
    15961 ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt
    16021 caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta
    16081 gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccattg ccagagctgc
    16141 gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc
    16201 gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac
    16261 cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct
    16321 cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac
    16381 acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata
    16441 cgcgataata cgcgcagcat gaccaatgtt gtgcacgccg gaagtacagg cggtcgcgat
    16501 agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac
    16561 aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt
    16621 gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg
    16681 ggttgcgttc tcttccgtta tttcaaggcc agaatcctgc atggcctgaa cgccagcgac
    16741 aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc
    16801 acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg
    16861 gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt
    16921 attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt
    16981 tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc
    17041 gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc
    17101 ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc
    17161 cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga
    17221 agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac
    17281 gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt
    17341 tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga
    17401 ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta
    17461 ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat
    17521 gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc
    17581 caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg
    17641 cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa
    17701 gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt
    17761 actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat
    17821 ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg
    17881 cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca
    17941 ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag
    18001 gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg
    18061 gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcac tgatcgcctg
    18121 agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc
    18181 agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa
    18241 attcatgatt ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag
    18301 cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ctttgcccgg
    18361 gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca
    18421 ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc
    18481 gcatttcaca tcaacgttat tcacaacagg aactgttggt gcgttaaagg tgattttcgc
    18541 taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct
    18601 cactggtaac ggcagagcgc gttttgcgcc cgcagcttta caggcagcgc cagcacgctc
    18661 aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga
    18721 aacgacctga ccttctgcag cttcttcaca cgctttcgca atagacgcat catccagacc
    18781 gatgattgcc gccatagcgc ccgtgccttc tggtacggct tcttgcatga acttgccgcg
    18841 catctcaacc agacgcaccg catcagcgaa atcaatcacg ccagcgcaaa ccagcgcgga
    18901 gtattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca
    18961 tacgcgatac agcgcaacag aggcagtcaa cagcgcaggc tgagtttgcc aggttttatt
    19021 cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga
    19081 agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc
    19141 aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt
    19201 atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag
    19261 aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac
    19321 agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat
    19381 accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca
    19441 gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag
    19501 ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc
    19561 tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg
    19621 cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc
    19681 atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc
    19741 ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct
    19801 taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat
    19861 gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt
    19921 tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt
    19981 cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg
    20041 gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc
    20101 gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta
    20161 cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt
    20221 cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata
    20281 cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct
    20341 gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc
    20401 cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc
    20461 tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc
    20521 cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta
    20581 acgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact
    20641 cattggcttc aagatagccg atataattga tagaagggat tgttttaagc accgctgagg
    20701 catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc
    20761 gaggattggg aatttccacc acctcttcag ccaggactga gcccataatg gcaaattgca
    20821 ccaacattgt gctgtcacaa tcgacgttgg cccctaaatc gagaaccacc gttttgccct
    20881 tttgctgatg cggcaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga
    20941 gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg
    21001 ctcgaccttc tttcaccaac tccagggcca cgcgcattga actcccacga ctggcgcgga
    21061 tagcttgcga aggccggaca tcactggcga taactgactg cgcaggaata atctgcagac
    21121 gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga
    21181 ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca
    21241 cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat
    21301 cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat
    21361 cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt
    21421 gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc
    21481 ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg
    21541 tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa
    21601 ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgcg cttcttcagg
    21661 cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg
    21721 aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc
    21781 gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga
    21841 acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg
    21901 ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt
    21961 atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc
    22021 gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt
    22081 acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact
    22141 ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg
    22201 cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca
    22261 aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat
    22321 atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg
    22381 caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca
    22441 ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat
    22501 ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt
    22561 aaaccgttaa cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc
    22621 accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg
    22681 gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt
    22741 aaagagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg
    22801 tttgagcgtt tagaggggcg tgatcctaac acgctggttg gtttaccgct aatcgcgctg
    22861 tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttttt tgcgccggat
    22921 gcggttactt cgcatccggc aatcggtgct ggcggcctga tgcggcgtaa acgccttatc
    22981 aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg
    23041 caaacaacgc ttcaaacctt catccatcgg cgcttcgatg cgcatcacct caccggtccc
    23101 cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc
    23161 ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg
    23221 acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg
    23281 caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc
    23341 ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc
    23401 ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg
    23461 cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctac
    23521 cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc
    23581 cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac
    23641 cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc
    23701 cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac
    23761 gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg
    23821 ttttttgttc acccgcactt cacctttacg caaaatacgg taaatcatac tttttggtac
    23881 gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat
    23941 agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac
    24001 aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact
    24061 acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg
    24121 agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa
    24181 tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat accccatttt
    24241 gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc
    24301 gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt
    24361 ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg
    24421 cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt
    24481 tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt
    24541 aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc
    24601 ttgtagatgg gcagcgtctg tatgacctgg atatcgaaag tccagggcac gagcagaaaa
    24661 aggcaaacat ctacaaaggt aaaatcaccc gtattgaacc gagtctggaa gctgcttttg
    24721 ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt
    24781 tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc
    24841 aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca
    24901 cctttatcag tctggcgggt agctatctgg ttctgatgcc gaacaacccg cgcgcgggtg
    24961 gcatttctcg ccgtatcgaa ggcgacgacc gtaccgaatt aaaagaagca ctggcgagcc
    25021 ttgaactacc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtcggc aaatctgctg
    25081 aggcgctgca atgggattta agcttccgtc tgaaacactg ggaagccatc aaaaaagccg
    25141 ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat
    25201 tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg
    25261 aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac
    25321 tgtacaccgg cgagatcccg ctgttcagcc actaccagat cgagtcgcag atcgaatccg
    25381 ccttccagcg tgaagtccgt ctgccgtccg gtggttccat tgttatcgac agcaccgaag
    25441 cgttaaccgc catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa
    25501 ctgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg
    25561 acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg
    25621 cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc
    25681 atatttctcg ctttggcctg ctggaaatgt cccgtcagcg cctgagccca tcactgggtg
    25741 aatccagtca tcacgtttgt ccgcgttgtt ctggtactgg caccgtgcgt gacaacgaat
    25801 cgctgtcgct ctctattctg cgtctgatcg aagaagaagc gctgaaagag aacacccagg
    25861 aagttcacgc cattgttcct gtgccaatcg cttcttacct gctgaatgaa aaacgttctg
    25921 cggtaaatgc cattgaaact cgtcaggacg gtgtgcgctg tgtaattgtg ccaaacgatc
    25981 agatggaaac cccgcactac cacgtgctgc gcgtgcgtaa aggggaagaa accccaacct
    26041 taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt
    26101 tcgctgaacg taagcgtccg gaacaacctg cgctggcaac ctttgccatg ccggatgtgc
    26161 cgccagcgcc aacgccagct gaacctgccg cgcctgttgt agccccagca ccgaaagctg
    26221 caccggcaac accagcaact cctgcacaac ctgggctgtt gagccgcttc ttcggcgcac
    26281 tgaaagcgct gttcagcggt agtgaagaaa ccaaaccgac cgagcaacca gcaccgaaag
    26341 cagaagcgaa accggaacgt caacaggatc gtcgcaagcc tcgtcagaac aaccgccgtg
    26401 accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag
    26461 aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc
    26521 agcaagctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg
    26581 aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga
    26641 atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc
    26701 cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag
    26761 aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag
    26821 ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac
    26881 cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt
    26941 ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg
    27001 agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg
    27061 cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag
    27121 agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactgaggtc cctgtcgccg
    27181 ccgctgtcga acctgttgtt agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag
    27241 cccccgtacc ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga
    27301 ttgccgccgc ggtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc
    27361 aggaagtcgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg
    27421 aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg
    27481 ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag
    27541 agatcaccgt tgagcataac cacgctaccg cgccaatgac gcgcgctccg gcaccggaat
    27601 atgttccgga ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta
    27661 aaggtgccgc aggtggtcat acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc
    27721 aacctgttga gtaataatta gctcaaagta atcaagccct ggtaactgcc agggcttttt
    27781 tatttcatct ttgaatcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa
    27841 tgcaaaatag gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac
    27901 ccagtttcat gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc
    27961 tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat
    28021 gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa
    28081 gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc
    28141 gcgcgaactg taaggacatt attcagcgag tttttcagtc cacggttggt tttatccaat
    28201 gccgcagcgg cgatttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg
    28261 atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct
    28321 accgcgttac tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa
    28381 cgcgaagcat cgacctgttg cctaatactt tctgctccac cgacgtattt ccctttctct
    28441 tcgctaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca
    28501 gtggtgttcg ccagattcag caactggtca cgaagcccct ggatatccgt agccagcgag
    28561 gcccggtcat cgtcactcaa agtgccattg ctggcgtaga caattttttc ctgagcattc
    28621 tggatagcag tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg
    28681 aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaaccgct
    28741 tgtgatgctg caatgggatc gtcagaaggg ttaacgactc gcttacctgt cgacatctgc
    28801 tcgccgtact tcatccattc cgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac
    28861 atcatctggg tactgaaacg catcttatct ccccttagcg aatgttaatc agcgcatcaa
    28921 aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt
    28981 gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa
    29041 gctgcgtcac cacattacct tgcgtggcgc tactggtttt caacgtcgcg gttttattac
    29101 cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt
    29161 tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttatcgcta tcacccgcat
    29221 cttcttcgct cgccatcgct attttcgctt cgtcggtgat taatacatcc atgttgacga
    29281 tagcgtcact tactggtttc agcgtgaagc tgtcgttaac agcaggcgtt cctgtaaacg
    29341 tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg
    29401 tattactggc aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatcag
    29461 tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtca cctttgtttt
    29521 tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcaccg gcatcgccgt
    29581 tagcatcaaa tccggctttg tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt
    29641 gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca
    29701 gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacgt
    29761 aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg
    29821 tacttccctg aaccagtgag taaccattgg ccatcgtgat gttataagta ccgccatcct
    29881 gaacgctgac ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat
    29941 ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt
    30001 tcaggctggc aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga
    30061 tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca
    30121 acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca
    30181 gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg
    30241 agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg
    30301 tctgcgcagc acgtaactgg ttggtaataa acgcatcata ctcacgctgc acaccagaaa
    30361 cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa
    30421 tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat
    30481 ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attaatcaag ctggacatgg
    30541 aggttcctta taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt
    30601 tattcagaac agattatcaa tgttcatact gtaggttttg ctcaccttgt cgcttatcga
    30661 tttcatctgc tgaatcatgt tggtgagttt gcgggcatag tgaggatcgg tggcatagcc
    30721 cgcgtcctgt agggcctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta
    30781 gcgcgggtta cgcgttaaca gcccaacgta atccgacaag gcttccagat acgagctgta
    30841 gacgcgaaac ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat
    30901 ttcagtaact ggccctttcc agttgccaga ggctttgaca ccaaacaggt tatagctcgg
    30961 ctcgccgttt tcgcggcgga tttgccgttg cccccaacca gattccagtg ccgcctgagc
    31021 gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag
    31081 ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca tcgtagttac gtggcacggc
    31141 cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacagttt cgagcgggaa
    31201 tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt
    31261 aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatttgctg
    31321 gtcatacata ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa
    31381 agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct ggcgggccac
    31441 cggacggata tttgccgccg gatcttcgcc cgctttcgcc tttagttcgt tgagtgattg
    31501 cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt
    31561 tttgcccgca gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc
    31621 gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg
    31681 ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc
    31741 tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgttact
    31801 gagagatttc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc
    31861 gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata
    31921 ttctggatat ctgcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga
    31981 atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca
    32041 gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg
    32101 acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg
    32161 ttcagttggt tcacctgaac actgctaccg ccagcggagg ctcctgcgcc gccaaccaga
    32221 atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg cgtcatcaac
    32281 aacgtacctc cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt
    32341 tgcccctgac gtccaaacgg aggaagtgac gctgtcacca ttaccgcagc gacgtttttt
    32401 agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc
    32461 gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc
    32521 agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata
    32581 cgctcagcct gagccgccgt cgtgaccagt agaagaatta atgcagagag aaatttaatc
    32641 accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat
    32701 tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag
    32761 tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa
    32821 taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt
    32881 tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg
    32941 ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac
    33001 caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac
    33061 ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca
    33121 gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa
    33181 tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt
    33241 gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg
    33301 aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca
    33361 tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta agccttagag ttgcgtcagt
    33421 ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc
    33481 acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct
    33541 tgatacagca gtcctgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accagaggat
    33601 tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg
    33661 gtgagattga gctgcccaac ctgaaccgaa gctgcctggc cttgttgggt tacgctgacc
    33721 acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc
    33781 acctgaaaac caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca
    33841 cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg ccctttaatc
    33901 gcgacatctt tgctgttgtt ggtctgcgac aggtttccct ggctgtgtaa gcgttcagtg
    33961 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    34021 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    34081 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    34141 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    34201 gtggattagt gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac
    34261 cctctcccca aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt
    34321 tcaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga
    34381 tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa
    34441 cggcattgac gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct
    34501 gcagtatcgg cccacgcgtg gcctggcttt ctgcgcttaa acgaaaaatg ccgtcgtcac
    34561 cgcgttgcac ttcgctgccc gtggctttca ccagtttcag acgccccact ggcgcaaccg
    34621 tatttgccgg atcgcccgga ttgagcgccg agattgtgcc atcggcagca atagtgattt
    34681 ccgccccttc cggcacagca attggcccag cctcgcctat caccggatgc ccctgaattg
    34741 tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt
    34801 cagcggactg cacggccagc cagccatcct gctgcaatgc aacgtccagc gggcgcgagg
    34861 tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc
    34921 gcgtgggcag tgaaagccct tccaccggca ctgcacgcaa ggcgtttaac tgcgcgcgaa
    34981 aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga ttcagcgtct
    35041 ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat
    35101 tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta
    35161 gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg
    35221 gacgcttcca acgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac
    35281 agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct
    35341 tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgtgtttg ttcgttggaa
    35401 tagttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc gcccggtttg
    35461 tagccattct gggtggttgc cacaatattg ttagcgccgg tattttgctg catggagttg
    35521 aggaagctca ggctaaacgt ggcgggatct gcgccgttaa ttgcgccagt ggtaatattc
    35581 tctgttggat ttgaggtcag aacgccattg gcattaaaca ccagcttcat tgcaggctcg
    35641 gctgtacctg ttggatcact gctatcctgg gtgtagacct gccagttatt atccccggtc
    35701 ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa
    35761 ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga
    35821 tcactggaat tcaggttgat ctgcatcgat gccgtggtgg tagttttcgc tgccatcagg
    35881 gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg
    35941 gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg gttttcatcc
    36001 agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac cagacggaaa
    36061 aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg
    36121 gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac
    36181 atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg
    36241 ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag
    36301 gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg
    36361 gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat cacaccctgc
    36421 accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc
    36481 gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg
    36541 aaactgtgaa ctccagcggt cagttcacca atatcaatgg tgcgcacaac cgcgccattt
    36601 ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc
    36661 gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt tgtgccgggg
    36721 atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt
    36781 ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt
    36841 gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc
    36901 tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc
    36961 gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt taccgcaatg
    37021 gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcata cttttcaccg
    37081 tgttgagcac ttcaacgttg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct
    37141 ccccgacgac atcaacgttc ggcattttta cgtagccctt tgcatctgcc agcggattac
    37201 ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc
    37261 cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt gcccgatatg
    37321 gctgtccatc aggaccggtc acgctatcag catttgccag attactggcc gccacgttca
    37381 ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca
    37441 tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc
    37501 taaggctcat ctggtattgc aggctgttat cggcaaactg ggtgcgttcg cgatccatat
    37561 cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag
    37621 gaggcgtcag cgcctgcgcc gggatgtgtt gcgttgaggt catcgtcagt gcaaccacac
    37681 tggttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc
    37741 gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct
    37801 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
    37861 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
    37921 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
    37981 tacaattcac gttgtagaaa tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg
    38041 cgatcatcgc gatactgttc agtccgttaa gtacggcgag caatctcaca tcgcaattgc
    38101 acaacttttt tagcgcccaa ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
    38161 cagcgcccaa tctactaccg ccatgcgagc agccattgct ttcgatgagc aataattccc
    38221 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
    38281 ttaatgtgca ggccacagga aattatgtgg ttgccgcgat gcccattgtg cggggaggaa
    38341 agctggaagc tggcaatgtc aaactaaaac gcggacggct ggataccctg ccaccgcgta
    38401 cggtgctgga tatcaatcaa cttgttgatg ccattagcct gcgcgatcta tcacccgatc
    38461 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
    38521 atgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
    38581 cagccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtagtc agcggcgttg
    38641 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
    38701 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaacc atgagtattg
    38761 atcgcacttc gcctctgaag cctgtaagca ccgttcaacc gcgcgaaacc actgacgcgc
    38821 cggtaacgaa cagccgggcg gcaaaaacaa ctgcctccac cagcaccagt gtgacgttaa
    38881 gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg
    38941 aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg
    39001 atgcgctgat caacgaagcg cagcaagact tgcagagtaa ctgatcgtat gacacgtctt
    39061 gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa
    39121 gagcagcaac atctctctat ggggcagatc aacggcagcc agttgcaatg gattacagaa
    39181 caaaaaagct cactgctggc gacgctggat tacctcgaac agttacgcaa gaaagaaccc
    39241 aatacagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag
    39301 caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat tgagcacaat
    39361 caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg ggcgaacggt
    39421 cagacctcaa caacccatcg cggcggtaaa aagatttcga tctgaatatt atctccggcc
    39481 tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaattctt taactttgaa
    39541 gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc gccagcacga ccgccattaa
    39601 acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc
    39661 taaaagcacg ccagacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca
    39721 gccgggttgc ggggtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt
    39781 cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccaataaa
    39841 cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt
    39901 aatgtcctgg cgggaataaa agccaggagc caaaactttc actacaatca ggccaatcaa
    39961 acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt
    40021 accgtactgg aacagcgaaa cggtcaacgg acctgaaaga atgcccaacg caaccgcact
    40081 cggcagcgcc aacaggaaac aaagacgcag cccccagtcc atcaaacggt tgtattcatc
    40141 atgattgcca ctggcaaaac tcttcgacag cgacggcagc aaaatggtgc caagcgccac
    40201 gcccagcaca ccagacggaa actccattaa gcggtcggcg taatacatcc aagacaccga
    40261 accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct ggctcacaga
    40321 gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa
    40381 gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag
    40441 ctgcaggatg ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta
    40501 cggtgcggca aacagcgcga aaccaatcat gctgatgtta agcagtgttg gagcaaacgc
    40561 cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat
    40621 cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc
    40681 tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc
    40741 gaccgtcaca accgccagcg cgagtgtcag caggccagaa acataagaga caaagacccg
    40801 cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga
    40861 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
    40921 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
    40981 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
    41041 cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
    41101 atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
    41161 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cacggcctgt tcgccggcgg
    41221 tttgcggaac tgtctgattt tgcacacatt caatgaagtg ccgcgcacag ccgacaaatc
    41281 cacgttgttc aagagtgctc tgccaaccag gaatcggttt atgcactacg ccctgcccgc
    41341 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct
    41401 gcacggtttc acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac
    41461 cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcgttagtc agtagcgtac
    41521 cgccatccag agaggctttg ccgcccgaca accacagcgc ggtatccacc acatgcagat
    41581 aatcatccag caacgtgaaa taaagatcgt gtggcccgac actattgcta cgatgtttat
    41641 ccattcttag cgaagctgcg gtagcgagtt gcgtttttaa ctcaccgtag agtggtgcga
    41701 aacgacggtt aaaaccgacc atcaacgtca gttttttacg cgccgccagt tccaccagcc
    41761 gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acacccgcat
    41821 tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat
    41881 cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa
    41941 ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacgtccag tcagacgctg
    42001 ccgccagcac cggtaaccac gctttttgcg caatgtcacc taatcccact acgccgatac
    42061 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt
    42121 tctgccactt cgatttccag ggcttcgacg cgggcctgta aatcaccatc aaccgcgttc
    42181 gacatatccg tcaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg
    42241 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattt
    42301 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg
    42361 gcgcggctgc gcagttcacc tggcgtctgg gcaccacgca ataacaacgt ggtgattaac
    42421 gccacttccg ctgcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
    42481 tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga
    42541 ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt
    42601 ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc
    42661 acctgctttt ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc
    42721 atgagattct ccttagcggc cgggcgtcca gtctggggtc gttaatgccg tcagtacgtg
    42781 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
    42841 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
    42901 atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag
    42961 tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc
    43021 gcgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa
    43081 accaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccca gcctggcctg
    43141 ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg
    43201 gcgattctct gcgtaataat ccgcaagacg ccaggcatca cgatcatgca ccagacgcac
    43261 gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct
    43321 atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac
    43381 cagaccctgt gaaaagtcca aaatgccatt ttctgcatta ttcacatatt tgataaaaac
    43441 tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct
    43501 tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg
    43561 cgtgtcgcag gcgaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt
    43621 gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg
    43681 ggccgccgtc atggtcggta ttgctctcgg tctacgccag tttattcagc aaggtctggg
    43741 tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat
    43801 gctgatgcgc gctgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctattgtg
    43861 gttttcatgc ctgctctcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct
    43921 ggtggtgaaa ctaatccgtc cacaacagcg tggtcgtttt ttctcgctgt tgatgatgca
    43981 ggacagtgcc ggtgcagtca ttggcgcatt gttggggagc tggctgttgc aatacgactt
    44041 tcgcctggtc tgcgccacag gggccgtttt gtttgtgctg tgtgcagcgt tcaatgcgtg
    44101 gttgttacca gcatggaaac tctccaccgt acgcacgccc gttcgcgaag gcatgacccg
    44161 cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct
    44221 ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcgccctc
    44281 tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat
    44341 cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat
    44401 aatgtcatta agcatgatgc cggtgggcat ggtcagcggc ctgcaacaac ttttcaccct
    44461 gatttgtctg ttttatatcg ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc
    44521 ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg tgtagccgtc tgggtctggc
    44581 gattggcggc gctattggtt atatcggtgg cggctggctg tttgacctgg gtaaatcggc
    44641 gcaccagcca gagcttccgt ggatgatgct gggcattatt ggcatcttca ctttccttgc
    44701 gctgggttgg cagtttagcc agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg
    44761 atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag
    44821 tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt
    44881 cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc
    44941 cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat
    45001 gccagaaagc atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac
    45061 cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa
    45121 actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc ccgccgcacg
    45181 caaatatttc gtcgacaaga aagaggccag cgcgggtaat tttgccgacc tgctggccca
    45241 ctctgacggt ctgattaaga atatcagcga tgatttacgt gcgctggata aactgatcgt
    45301 caaaccgaac gccgtgaatg gcgaactttc agaagatgat attcagctat ttccgctact
    45361 acgtaatctg acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga
    45421 taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc
    45481 cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggtgagtt
    45541 tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga
    45601 ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct
    45661 gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac
    45721 ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga
    45781 caaacctgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg
    45841 ccaatctgga catgaactcc ctgttcggta gccagaaagc gaccatgaaa ctgaagctga
    45901 aagcgctgcc cgtgtttgat aaagaaaaag gcgcgatctt cctgaaagag atggaagtgg
    45961 tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt
    46021 tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg
    46081 gcagccaggg cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg
    46141 aaattgtcat tccatttact gattaatcac gagggcgcat tcgcgccctt tatttttcgt
    46201 gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag
    46261 catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc
    46321 accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt
    46381 atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg
    46441 tggcgtatcg ccaacgtatt cttgatgccg tacctgccgg gcacaatttc accccattga
    46501 tgacctgtta tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaacgaag
    46561 gcgtgttcac cgctgcaaaa ctttatccgg caaacgcaac cactaactcc agccacggcg
    46621 tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc
    46681 tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct
    46741 ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg
    46801 agcacatcac caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg
    46861 ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc
    46921 gtccgcacct gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg
    46981 aactggtcgc cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac
    47041 gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg
    47101 gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt
    47161 ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga cacattcatc gaactggtac
    47221 gtgaagagca acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc
    47281 tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca
    47341 ttaggttatt ttgcctgtat aaataaccag tatattcaac agggggctat tatgcgaatt
    47401 gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc
    47461 gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt
    47521 tatgccgcag cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt
    47581 agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat
    47641 gcagtgattt ttgtttttgc cgggtcgccc cggctttttt attttactta caaattttga
    47701 gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga
    47761 catgctgttt tattgttcat ataaatctta aaagcagaaa tttatctgtt ccccccttta
    47821 aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat
    47881 tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa
    47941 tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga
    48001 tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga
    48061 agttggtcag acgctctggt taaccactga tgttgccaga caatttattt cgatattaga
    48121 agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta
    48181 acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg
    48241 tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa
    48301 atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac
    48361 ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag
    48421 ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc
    48481 gttggtcctc cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg aagatgatcc
    48541 catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat ttctcgccaa
    48601 cgtcgcccac agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat
    48661 tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga
    48721 ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc
    48781 gggctgtgcg caactgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt
    48841 aactattgaa accgttgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac
    48901 atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgtaaag tatttgcctg
    48961 gtatcaggcc gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact
    49021 gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa
    49081 acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt
    49141 cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg
    49201 cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac
    49261 cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt
    49321 tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga
    49381 tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg
    49441 ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc
    49501 gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat
    49561 gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca
    49621 tttggcgtga aaaattgaat gagattattc ctgcttctac agcactcacc agacatctct
    49681 gatgct
//
LOCUS       DAZFFI010000024        48142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFFI010000024 DAZFFI010000000
VERSION     DAZFFI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.024"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            286..1602
                     /gene="fucP"
                     /locus_tag="ACHJ12_001683"
     CDS             286..1602
                     /gene="fucP"
                     /locus_tag="ACHJ12_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4989482.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1635..3410
                     /gene="fucI"
                     /locus_tag="ACHJ12_001684"
     CDS             1635..3410
                     /gene="fucI"
                     /locus_tag="ACHJ12_001684"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4989483.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQKEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3519..4937
                     /gene="fucK"
                     /locus_tag="ACHJ12_001685"
     CDS             3519..4937
                     /gene="fucK"
                     /locus_tag="ACHJ12_001685"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4989484.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4939..5361
                     /gene="fucU"
                     /locus_tag="ACHJ12_001686"
     CDS             4939..5361
                     /gene="fucU"
                     /locus_tag="ACHJ12_001686"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4989485.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5419..6150
                     /gene="fucR"
                     /locus_tag="ACHJ12_001687"
     CDS             5419..6150
                     /gene="fucR"
                     /locus_tag="ACHJ12_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4989486.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACHJ12_001688"
     CDS             complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACHJ12_001688"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4989487.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACHJ12_001689"
     CDS             complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACHJ12_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4989488.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACHJ12_001690"
     CDS             complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACHJ12_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4989489.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACHJ12_001691"
     CDS             complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACHJ12_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4989490.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9388..10593
                     /gene="csdA"
                     /locus_tag="ACHJ12_001692"
     CDS             9388..10593
                     /gene="csdA"
                     /locus_tag="ACHJ12_001692"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4989491.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10593..11036
                     /gene="csdE"
                     /locus_tag="ACHJ12_001693"
     CDS             10593..11036
                     /gene="csdE"
                     /locus_tag="ACHJ12_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4989492.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACHJ12_001694"
     CDS             complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACHJ12_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4989493.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACHJ12_001695"
     CDS             complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACHJ12_001695"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4989494.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13437..13513
                     /locus_tag="ACHJ12_001696"
     tRNA            13437..13513
                     /locus_tag="ACHJ12_001696"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13471..13473,aa:Met,seq:cat)
     gene            complement(13588..14841)
                     /gene="amiC"
                     /locus_tag="ACHJ12_001697"
     CDS             complement(13588..14841)
                     /gene="amiC"
                     /locus_tag="ACHJ12_001697"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4989495.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15073..16404
                     /gene="argA"
                     /locus_tag="ACHJ12_001698"
     CDS             15073..16404
                     /gene="argA"
                     /locus_tag="ACHJ12_001698"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4989496.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16466..18292)
                     /gene="recD"
                     /locus_tag="ACHJ12_001699"
     CDS             complement(16466..18292)
                     /gene="recD"
                     /locus_tag="ACHJ12_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4989497.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18292..21834)
                     /gene="recB"
                     /locus_tag="ACHJ12_001700"
     CDS             complement(18292..21834)
                     /gene="recB"
                     /locus_tag="ACHJ12_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4989498.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21827..24715)
                     /gene="ptrA"
                     /locus_tag="ACHJ12_001701"
     CDS             complement(21827..24715)
                     /gene="ptrA"
                     /locus_tag="ACHJ12_001701"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4989499.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24891..28259)
                     /gene="recC"
                     /locus_tag="ACHJ12_001702"
     CDS             complement(24891..28259)
                     /gene="recC"
                     /locus_tag="ACHJ12_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4989500.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESAMTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQDLDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28272..28595)
                     /gene="ppdC"
                     /locus_tag="ACHJ12_001703"
     CDS             complement(28272..28595)
                     /gene="ppdC"
                     /locus_tag="ACHJ12_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4989501.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28580..28987)
                     /gene="ygdB"
                     /locus_tag="ACHJ12_001704"
     CDS             complement(28580..28987)
                     /gene="ygdB"
                     /locus_tag="ACHJ12_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4989502.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(28984..29547)
                     /gene="ppdB"
                     /locus_tag="ACHJ12_001705"
     CDS             complement(28984..29547)
                     /gene="ppdB"
                     /locus_tag="ACHJ12_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4989503.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29538..30008)
                     /gene="ppdA"
                     /locus_tag="ACHJ12_001706"
     CDS             complement(29538..30008)
                     /gene="ppdA"
                     /locus_tag="ACHJ12_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4989504.1"
                     /translation="MKTQRGYTLIETLVAMLILVMQSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30193..30987)
                     /gene="thyA"
                     /locus_tag="ACHJ12_001707"
     CDS             complement(30193..30987)
                     /gene="thyA"
                     /locus_tag="ACHJ12_001707"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4989505.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30994..31869)
                     /gene="lgt"
                     /locus_tag="ACHJ12_001708"
     CDS             complement(30994..31869)
                     /gene="lgt"
                     /locus_tag="ACHJ12_001708"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4989506.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32020..34266)
                     /gene="ptsP"
                     /locus_tag="ACHJ12_001709"
     CDS             complement(32020..34266)
                     /gene="ptsP"
                     /locus_tag="ACHJ12_001709"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4989507.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34279..34809)
                     /gene="rppH"
                     /locus_tag="ACHJ12_001710"
     CDS             complement(34279..34809)
                     /gene="rppH"
                     /locus_tag="ACHJ12_001710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4989508.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35162..35308)
                     /gene="ygdT"
                     /locus_tag="ACHJ12_001711"
     CDS             complement(35162..35308)
                     /gene="ygdT"
                     /locus_tag="ACHJ12_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4989509.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35494..36183
                     /gene="mutH"
                     /locus_tag="ACHJ12_001712"
     CDS             35494..36183
                     /gene="mutH"
                     /locus_tag="ACHJ12_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4989510.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36252..36965
                     /gene="ygdQ"
                     /locus_tag="ACHJ12_001713"
     CDS             36252..36965
                     /gene="ygdQ"
                     /locus_tag="ACHJ12_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4989511.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37103..37321
                     /gene="ygdR"
                     /locus_tag="ACHJ12_001714"
     CDS             37103..37321
                     /gene="ygdR"
                     /locus_tag="ACHJ12_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4989512.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37429..38469
                     /gene="tas"
                     /locus_tag="ACHJ12_001715"
     CDS             37429..38469
                     /gene="tas"
                     /locus_tag="ACHJ12_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4989513.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38501..39694)
                     /gene="lplT"
                     /locus_tag="ACHJ12_001716"
     CDS             complement(38501..39694)
                     /gene="lplT"
                     /locus_tag="ACHJ12_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4989514.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQYELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39687..41846)
                     /gene="aas"
                     /locus_tag="ACHJ12_001717"
     CDS             complement(39687..41846)
                     /gene="aas"
                     /locus_tag="ACHJ12_001717"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4989515.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42432..43463
                     /gene="galR"
                     /locus_tag="ACHJ12_001718"
     CDS             42432..43463
                     /gene="galR"
                     /locus_tag="ACHJ12_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4989516.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43470..44732)
                     /gene="lysA"
                     /locus_tag="ACHJ12_001719"
     CDS             complement(43470..44732)
                     /gene="lysA"
                     /locus_tag="ACHJ12_001719"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4989517.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44854..45789
                     /gene="lysR"
                     /locus_tag="ACHJ12_001720"
     CDS             44854..45789
                     /gene="lysR"
                     /locus_tag="ACHJ12_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4989518.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45776..46468)
                     /gene="ygeA"
                     /locus_tag="ACHJ12_001721"
     CDS             complement(45776..46468)
                     /gene="ygeA"
                     /locus_tag="ACHJ12_001721"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4989519.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLMEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46597..48015)
                     /gene="araE"
                     /locus_tag="ACHJ12_001722"
     CDS             complement(46597..48015)
                     /gene="araE"
                     /locus_tag="ACHJ12_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4989520.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11790 a        13065 c        11936 g        11351 t
ORIGIN      
        1 gcataaacaa ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg
       61 gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa
      121 gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt
      181 tcagacaggc aaatattcac tgatatgaag cccgaactcg ctggttttgc acttttgaaa
      241 acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca
      301 atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt
      361 attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac
      421 gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca ggctggcctg
      481 atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg gatattgatg
      541 aaaaaactca gttataaagc agggattatt accggattat ttttatatgc cttgggcgct
      601 gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt tggcctattt
      661 attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt tacggtatta
      721 gggccagaaa gcagtggtca cttccgctta aatcttgcgc aaacatttaa ctcgtttggc
      781 gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg
      841 caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta
      901 ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg
      961 atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga
     1021 tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg ggcggtatta
     1081 gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct
     1141 gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac cggaaccatg
     1201 gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac
     1261 aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct
     1321 ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac
     1381 ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc
     1441 ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg ttttgtcagt
     1501 gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc
     1561 tttatctttg cccgtttccg ctctcaaacg gcaactaact gaacatattt tccgaataaa
     1621 gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc ccggttattg
     1681 acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag
     1741 ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg
     1801 tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa gaaaaattca
     1861 gcagtcagaa tgtaggcctc accattacgg taacgccttg ctggtgctat ggcagtgaaa
     1921 ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc actgaacgtc
     1981 ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc atcccggcat
     2041 tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg
     2101 aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa
     2161 gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttccattgtt gatcacaact
     2221 tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc
     2281 gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc tgggctgata
     2341 aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat gccgagcaaa
     2401 gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag
     2461 gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg
     2521 ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt gacaccgccg
     2581 aagcgatcct taacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga
     2641 ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg
     2701 ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg
     2761 ggcataaact cgatggactg gcagaacacg gcatcatcca tttgatcaac tccggttctg
     2821 ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc
     2881 actgggaaat ctctcagaaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg
     2941 cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg
     3001 tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg
     3061 cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca
     3121 actcaacctg gccaaccacc tggtttgcac cgcgcctcac cggtaaaggg ccgtttacgg
     3181 atgtgtactc ggtaatggcg aactggggcg ctaaccatgg ggttctgacc atcggccacg
     3241 ttggcgctga ctttatcact ctcgcctcca tgctgcgtat cccggtatgt atgcacaacg
     3301 ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag
     3361 gccaggatta ccgcgcttgc cagaactacg gcccgttgta caagcgttaa tacattactc
     3421 cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag tggttagccg
     3481 gataagcaat gttatccggc tatattgcag gagcgattat gaaacaagaa gttatcctgg
     3541 tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg
     3601 ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc
     3661 agtggtcttt agacgccatt ttgcaacgct ttgctgattg ctgtcggcaa atcaatagtg
     3721 aactgactga atgccacatc cgcggtatcg ccgtcaccac ctttggtgtg gatggcgctc
     3781 tggtagataa gcaaggcaat ctgctctatc cgatcattag ctggaaatgt ccgcgaacag
     3841 cagcggttat ggacaatatt gaacagttga tctccgcaca gcggttgcag gctatttctg
     3901 gcgtcggagc ctttagtttc aatacgttat ataagttggt gtggttgaaa gaaaatcatc
     3961 cacaactgct ggaacgcgcg cacgcctggc tctttatttc gtcgctgatt aaccaccgtt
     4021 taaccggcga attcactact gatatcacga tggccggaac cagccagatg ctggatatcc
     4081 agcaacgcga tttcagtccg caaattttac aagccaccgg tattccacgc cgactcttcc
     4141 ctcgtctggt ggaagcgggt gaacagattg gtacgctaca gaacagcgcc gcagcaatgc
     4201 tcggcttacc cgttggcata ccggtgattt ccgcaggtca cgatacccag ttcgcccttt
     4261 ttggcgctgg tgccgaccaa aatgaacccg tgctctcttc cggtacatgg gaaattttaa
     4321 tggtccgtag cgcccaggtt gatacttcgc tgttaagtca gtacgccggt tccacctgcg
     4381 aactggatag ccaggcaggg ctgtataacc caggtatgca atggctggca tccggcgtgc
     4441 tggaatgggt gagaaaactg ttctggacgg ctgaaacacc ctggcaaatg ttgattgaag
     4501 aggctcgtct gatcgcgcct ggcgcggatg gcgtgaaaat gcagtgtgat ttattgtcgt
     4561 gtcagaacgc tggctggcaa ggagtgacgc ttaataccac gcgggggcat ttctatcgcg
     4621 cggcgctgga agggttaact gcgcaattac agcgcaatct acagatgctg gaaaaaatcg
     4681 ggcactttaa ggcctctgaa ttattgttag tcggtggagg aagtcgcaac acattgtgga
     4741 atcagattaa agccaatatg cttgatattc cggtaaaagt tctcgacgac gccgaaacga
     4801 ccgtcgcagg agctgcgctg ttcggttggt atggcgtagg ggaatttaac agcccggaag
     4861 aagcccgcgc acagattcat tatcagtacc gttatttcta cccgcaaact gaacctgaat
     4921 ttatagagga agtgtgaaat gctgaaaaca atttcgccgt taatttctcc cgaactattg
     4981 aaagtgctgg cagagatggg acatggagat gaaattattt tttccgatgc tcactttccc
     5041 gcccattcga tgggaccgca ggtgatccgc gctgatggcc tgttggtgag cgacttgctc
     5101 caggcgatta tcccgttatt tgaactggac agttatgcac cgccgctggt gatgatggcg
     5161 gcggtagaag gtgacactct cgatcctgaa gtagaacgac gttaccgtaa tgcgctttca
     5221 ctacaagccc cgtgtcctga catcatccgc atcaatcgtt ttgcgtttta tgaacgggcg
     5281 caaaaagcct ttgcgatcgt tatcacaggc gaacgagcga agtacgggaa tattctttta
     5341 aaaaaagggg taacaccgta atctcatacc ggtacgcccg catgacgcgg gcggttatcg
     5401 aatgatgggg tgaaaaatat gaaagcggca cgccagcaag cgatagtcga cctgctgctg
     5461 aaccatacca gcctgaccac ggaagctctc tctgaacagc taaaggtcag taaagaaacc
     5521 attcgtcgcg atctcaatga attacagacg cagggtaaaa ttctgcgcaa tcatggacgc
     5581 gctaaatata tccaccgtca aaatcaagac agtggcgatc cctttcacat caggctgaaa
     5641 agccattatg cgcataaagc agatatcgcg cgcgaggcgc tcgcgtggat tgaagaaggg
     5701 atggtgatag ccttagacgc cagttcaact tgctggtatc tggcacgcca gttgcctgac
     5761 atcaacattc aggtcttcac caatagccat ccgatttgcc atgaactcgg taaacgcgaa
     5821 cgcattcaac tgatcagttc cggcggcaca cttgagcgca aatatggctg ttacgtcaat
     5881 ccctcgctga tttcccaact taaatcgctg gaaatcgatc tgtttatttt ttcttgtgaa
     5941 gggatcgata gcagcggcgc actgtgggac tccaatgcga tcaacgctga ttacaaatcg
     6001 atgctattaa aacgtgccgc gcaatcgttg ttattgattg ataaaagtaa atttaatcgt
     6061 tcaggggaag cccgcatcgg gcatctggat gaggtaacgc acattatttc tgatgagcgc
     6121 caggttgcaa cttctttggt aacagcctga cggaaagggt agcaggccgg agacgacgcc
     6181 ccggccttgc ctgttatcgc tcgtcgcgac gaccacccac cgcagcccag atgcggcgga
     6241 cgtgcaccgt cacttcttcg cgatcgtgat acaactgccg cgcctgaatc tgagcattta
     6301 tgccatgttc atcaagctgt gcctgaatat acgccagatt gtgtgacact tcttcgtagc
     6361 gttttttcat cggcagtttg aggttgaaaa tggtttcacg gcaccagcca ttaaccagcc
     6421 actgcgccat caatgccgca actttcgccg gtttttcaac catatcgcat accatccagg
     6481 agatattgct gcgcgtcgga cggaatttga aaccgtcttc ccgcagccac gtcacctgtc
     6541 cggtatccat cagactttgc gccatcgggc cgttgtcgac ggaataaacc cacatgttgc
     6601 gcttcaccag ttggtaggtc cagccgccag ggcaagcgcc taaatccacc gcccacatcc
     6661 cgttcgccag gcgttcatcc cactcatccg caggaataaa cacatgaaat gcctcttcca
     6721 gtttgagcgt ggaacgactc ggcgcatctg ccggaaattt caggcgcgga atgcccatat
     6781 agaacggcga attattgttg ctgtatgagt aaccggtata gcagcagcct ggtgcaataa
     6841 agaatacatg cacaaccgga cgcttcggcg tttcatagtt cgccagcacc ccggcatcgc
     6901 gcaaggcagc gcgtagcgga acagtaaatt tacggcagaa tttcagtaac tctttgcttt
     6961 cgttggtatc ggcaacttca acacgcagtt caccgccctt ctctactacg ccctgtaaca
     7021 tgccgacaat gggggtaata cgatcttctg gcggcaaatg ctgcaggagt tcccccacca
     7081 caaaccactg gcgggcaaaa attaatgaac tgaacggcag ctcacggatt aacttatcgc
     7141 catcatcagg ttgataacat tcataaatga cataacccgc attctctttc acgcgggcaa
     7201 aaccgaaaat ttcccgctgg ccggctttat cggtaatttc tgcggcgcac tctttttcaa
     7261 agcccggacg gcacagcaat acaaccttat tcatgactta cgcccttgcg ctttaaacgg
     7321 atagcaccaa ctaacattaa cgcccagccc gcgaggaagc tcacgccgcc aaccggagtg
     7381 acaaacgccc acaaacgcag atgggacagc gccaggcaat aaaggctgcc gctgaacaac
     7441 accgtgccta acgcgaggaa aacgctactc cagtaaaacc agatactgat gcgacgctgc
     7501 attgccaccg ccagacctaa gatcgccagc gtatgaaacg cctggtattc gaggccggtc
     7561 tggatccagc ccatctcaac ggcccccatg gttttactta acacatgcgc gccaaaagcg
     7621 cccagagcca caaaaatgaa gccgctaatg gcggcgaaaa tcagcataaa acggctggtc
     7681 atggtcgtac cctacgtaaa ttattgttca taacgaaagc ggaatttttc ttgttcagca
     7741 gcggctttcg ccaggatcca ttggcgaaag gcggctattt tacccagttc tgcctggctg
     7801 tcatgacaaa ccagataaaa agcgttttta ctgaccagaa catcattaaa cgggcaaaca
     7861 agacgtccgg cctcgatttc agattgcgcc atcacgttat ttgccagcgc cactccctgc
     7921 ccgtgaatag ccgcttgcag caccatggcg ctatgactaa aaattggccc ttgctgaacg
     7981 ttgatatgat ttaaccccaa ctgtcgggta tatgtctgcc agtcacggcg tgacgcatca
     8041 tgtaataacg tatgtttagc cagatcttcc ggggtcttca agggtttttc gcctgtcagc
     8101 agtagcggcg aacacaccgg caataaatat tcggcgtaca gtttttccac ccgtagcccc
     8161 ggccagttgc cccgaccata aaatatcgcc acatcaacat catccgccag cttatcttcc
     8221 tgacgatcaa ccgcctggat tcgaacgtca attcccggat aagctgaatt aaagctggaa
     8281 agtcgcggaa ccaaccaatg aatggcaaaa ctggggagta aactgaccgt caacgccccc
     8341 ttggcgctac gggcctggag tttacgcgtc gcttcggtta attgcgaaaa tatctctttg
     8401 atatcgagga aatagctttg cccttcctcg gtcagcagga gtgaacgatt acggcggcgg
     8461 aacagtttta gccccaaaaa atcctcaaga gacttgattt gatgacttac tgcggcctgg
     8521 gtcacaaaaa gctcttctgc tgcgcgagtg aaacttaaat ggcgtgctgc ggcatcaaaa
     8581 actcgtaagg catttagcgg tggtaatcgt ttagacatgg cttttaaact ttgatgttaa
     8641 atgaatttaa caattagatt acactgtgta acctattagt ttttttaatc tgagccatta
     8701 taaattgtcc gttgagcttc taccagcaaa tacctatagt ggcggcactt cctgagccgg
     8761 aacgaaaagt tttatcggaa tgcgtgttct ggtgaacttt tggcttacgg ttgtgatgtt
     8821 gtgttgttgt gtttgcaatt ggtctgcgat tcagaccatg gtagcaaagc tacctttttt
     8881 cacttcctgt acatttaccc tgtctgtcca tagtgattaa tgtagcaccg cctaattgcg
     8941 gtgctttttt ttaccttgcg atcgcgaatt actgatccag ttcgaccatc tctttcacgt
     9001 cagtacggtt gatctgctgt ttgttgccat tagcgtcttt atacgaaatc ataccggtat
     9061 cgttatcagt ctgtggtttg ccgtcagaga cgatggtacg tccgtcattg gtgtgcatca
     9121 catagttcga accggaacag gcgctcaggg caaaagtcag catacaggca gaaataattg
     9181 cggcagtctt tttcatcatc ttctccttaa agcgattaat aaatcttaat agtattgatt
     9241 aacaggctaa aattaacgcc taacactatt cagcatatgt tacttacgcg gcttcgccag
     9301 gatatccaga taattctgat ggttagcact ctccttgtat caaagtgaat tttgcgtcac
     9361 gatcggtgca tcaagccgag gagtaccatg aacgttttta atcccgcgca gtttcgcgcc
     9421 cagtttcccg cactacagga tgcgggcgtc tatctcgata gcgccgcgac cgcgcttaaa
     9481 cctgaagccg tggttgaagc cactcgacag ttttacagcc tgagcgccgg aaacgtccat
     9541 cgcagccagt ttgccgaagc ccaacgcctg accgcgcgtt atgaagctgc acgagagaaa
     9601 gtggcgcaat tactgaatgc accggatgat aaaactatcg tctggacgcg cggcaccact
     9661 gaatccatca acatggtggc acaatgctat gcgcgtccgc gtctgcaacc gggcgatgag
     9721 attattgtca gcgtggcaga acaccacgct aacctcgtcc cctggctgat ggtcgcccaa
     9781 caaactggag ccaaagtggt gaaattgccg cttaatgcgc agcgactgcc ggatgtcgat
     9841 ttgttgccag aactgattac tccccgtagt cggattctgg cgttgggtca gatgtcgaac
     9901 gttactggcg gttgcccgga tctggcgcga gcgattacct ttgctcattc agccgggatg
     9961 gtggtgatgg ttgatggtgc tcagggggca gtgcatttcc ccgcggatgt tcagcaactg
    10021 gatattgatt tctatgcttt ttcaggtcac aaactgtatg gcccgacagg tatcggcgtg
    10081 ctgtatggta aatcagaact gctggaggcg atgtcgccct ggctgggcgg cggcaaaatg
    10141 gttcacgaag tgagttttga cggcttcacg actcaatctg cgccgtggaa actggaagct
    10201 ggaacgccaa atgtcgctgg tgtcatagga ttaagcgcgg cgctggaatg gctggcagat
    10261 tacgatatca accaggccga aagctggagc cgtagcttag caacgctggc ggaagatgcg
    10321 ctggcgaaac gtcccggctt tcgttcattc cgctgccagg attccagcct gctggccttt
    10381 gattttgctg gcgttcatca tagcgatatg gtgacgctgc tggcggagta cggtattgcc
    10441 ctgcgggccg ggcagcattg cgctcagccg ctactggcag aattaggcgt aaccggcaca
    10501 ctgcgcgcct cttttgcgcc atataataca aagagtgatg tggatgcgct ggtgaatgcc
    10561 gttgaccgcg cgctggaatt attggtggat taatgacaaa cccgcaattc gccggacatc
    10621 cgttcggcac aaccgtaacc gcagaaacgt tacgcaatac cttcgcaccg ttgtcgcaat
    10681 gggaagataa atatcgccag ttgatcatgc tggggaaaca gcttccggct ttgccagacg
    10741 agttaaaagc gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg ctgggatata
    10801 ccgtgactga aaacggtaaa atgcacttct ttggcgacag cgaagggcgc attgtgcgcg
    10861 gcctgttggc ggtgttgttg actgccgttg aggggaaaac cgtcgccgaa ttgcaggcac
    10921 agtcgccgct ggcattgttt gatgagctgg gattgcgtgc acagcttagc gcctcacgca
    10981 gccaggggtt gaatgcgtta agcgaggcga ttatcgctgc ggcgaagcag gtttaagcac
    11041 agaaccaggt cggataagac gttcgcgtcg catccgacct gattgtttaa ccctgacgcg
    11101 ccgctttcgc catcatcttc ttcagcgcat gagaaaccgc aacaaaccca aaggtggcgg
    11161 tcaccatcgt tgccgcgcca aatccagatg cacaatccat ccgcttcggc ccttctgccg
    11221 tggctttcat cgcacacacc gtaccgtctg actgcgggta caccagcgct tcagtagaaa
    11281 acacgcaatc cacgccgagc ttacctttac tgtttttcac tacgccaaaa tcgcttttca
    11341 ggcgctcgcg caacttcgcc gccagcgggt cctgaatcgt tttcgccaga tcggtaacct
    11401 gaatctgcgt cgggtcaatc tgcccacccg cgccaccggt cgtgaccagc gggattttat
    11461 tgcgccgaca ataagcaatc agcgccgctt tgggtcgtac actatcaatg gcatcaatca
    11521 cgtacgaata accggcactc atatactgcg ctacgttatc cggcgtcacg aaatcatcca
    11581 ccaccgttac acggcactcc gggttaatct ggcgaatacg ttccgccatg actttcgctt
    11641 ttgccagccc aacgttatcg cgcagggcat gaatttgccg attggtattg gtgacgcaca
    11701 catcatccat atcgataagc gtgattgcgc caatccccgt gcgcgccagc gcttccgccg
    11761 cccaggaacc gacaccaccg ataccgacca cacaaatatg cgcgtcagca aacagttgca
    11821 acgctttttc accatacaga cgcgctgtgc caccaaaacg ctgacgccat gcatcactaa
    11881 ttaccacaga catagcacct cagaattaaa aagggtgagg ttttccctca ccctaaacgc
    11941 ataaatgcct gatgcgctac gcttatcagg cctacataac tcctgcaata ttttggattt
    12001 gcatgatttg tgggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt
    12061 agtcaataat caggatgtga ggttacccct caccctgtca tatccgtaaa aacggcatac
    12121 agaatatcac atcagccgct aaagacgtta cctgcgcccg gggcggtttt cagcacccag
    12181 acacgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
    12241 tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac catcagacgc
    12301 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgccagcaag
    12361 gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc
    12421 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg
    12481 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
    12541 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
    12601 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
    12661 ccaaagtcga tatacccgct accctgcaca tccataatga agttatccat cagggagttg
    12721 ctgtaagcga gaatatattt atcactcagc gcccctgcgt agatctccgc acgagacggc
    12781 agacgaccac gttttggcgg catacggtaa atcggatact ggaactcgcc ctggcgggta
    12841 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
    12901 gcaccttcca tctgccaggc atcaatgccg aactgacgca tattgcgggt atcaccgcct
    12961 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
    13021 gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca
    13081 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc
    13141 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
    13201 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
    13261 caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
    13321 ttgcacaaaa aataggctta gtgatttgtt tttgttcaaa atcatgccaa atccgtgatc
    13381 ggggtaaaaa aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggacgcg
    13441 gggtggagca gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg
    13501 gcccccgcaa ccaattattg aacaccctaa cgggtgtttt tttgtttctg ctctcccata
    13561 aaaaaagcgc cattcagcgc ctttttatca tccccttctc gctagcgtcg ccccatcggc
    13621 aaaatacgct ttaattcccg caagaataga ctccgcaact tcctgctgga aagtcgccgt
    13681 tttcagttta cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg
    13741 aatatcaggc gctttcagca cggcaaaccc ggcctgttca acctgatttt tatgcagctt
    13801 gttgatttta ccgagcttat tcagcaccgc tttaccaaac ttaaggctgt cggcaatggt
    13861 cagcgactgc accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac
    13921 gccaccaatc aagtccgagg cgttctgggt ctgtgccaga tattttgccg cagtactggt
    13981 tgcgcctttg gttgagagcg caaacactga ggaaccgctc ggctggcgac tggtaaaggc
    14041 gtcggcatgg atagagacaa acaggtcggc acgctgtttc tgggcttttg ctacgcgcac
    14101 ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt
    14161 ctcgatcaga gagcgcagac ggcgagctat ttgcaatacc acgtcttttt cgcgcgtttt
    14221 gtatttcccc accgcaccgg agtcttcgcc gccgtggcca gggtcaagca taataacaat
    14281 aggacgatcg cgccctgctt tacccggttg tggaccactt tgtgctggcg gcacctgctt
    14341 ttcgaggtcg cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg
    14401 tgcattggcc ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag
    14461 ggcaaacagc tgcggtttta cgttttgctt taattcaaaa accatccgta cggtctgcgg
    14521 gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc
    14581 catccccttg agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttccgg
    14641 gttactcaac gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt
    14701 gtagctggac gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac
    14761 ctgacttacg ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat
    14821 tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag
    14881 taaacgacta atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac
    14941 ggtaaacatt ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat
    15001 ttacacttgc accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taataatgca
    15061 aaggggtgtg ccgtggtaaa ggaacgtaaa accgagttgg tcgagggatt ccgccattcg
    15121 gttccctata tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc
    15181 attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc
    15241 atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat
    15301 caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg
    15361 gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc
    15421 aataacacgc cgctacaggg cgcgcatatc aacgtcgtta gtggcaattt tattattgcc
    15481 cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt
    15541 gatgaagacg cgatccatcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc
    15601 gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaacta
    15661 gccatcaaac tgaaagctga aaagatgatt ggtttttgct cttcccaggg cgttactaat
    15721 gacgacggtg atattgtctc agaacttttc cctaacgaag cgcaagcgcg ggtagaagcc
    15781 caggaagaga aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa
    15841 gcctgccgca gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg
    15901 ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag
    15961 cagattcgtc gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg
    16021 gagcagcaag gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa
    16081 ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta tccgttcccg
    16141 gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca
    16201 cggggcgagg ttctgctgga acgcattacc gctcaggcga agcagagcgg cttaagcaaa
    16261 ttgtttgtgc tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg
    16321 gatattgatt tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa
    16381 gtgttgatgg cggatttagg gtaagcgatg aaaattgtcg gatgcgacat gcgtaacact
    16441 cgtgcgtcgc atccggcaat tacgtttatt cccgcgaact aaacaacgcc gccagaccac
    16501 tgcgccgctc agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg
    16561 acagacggcg acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actaccggcg
    16621 tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta
    16681 ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat
    16741 tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga
    16801 tatcgccatt aaacaaccca agcgcgctgt cattacgggc aatcattacc ggtcgacctt
    16861 cgtaccaacg agagtgcgga tgacgatgaa ttttgcgctt ctgttgcata aactgctcaa
    16921 ttcgctcatt cagtccagcc acgccaaacg gcccttcccg cagggcgcac aaaagttggt
    16981 actcattgaa cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat
    17041 aacgtccata acccgcaaga gcttcctcaa gcatcgcaat ataatcttct ccgctctgta
    17101 aaagccgttt ttcgatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat
    17161 caccacggtt gatcgccgca gctaactgac caatgccaga atcactgccg aaacgatagc
    17221 ttttttgcag caggcagaga ctgtcgcgca aagatgccgc ttctgtgcca gttcctgccg
    17281 gaacgtgagt tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat
    17341 tggcataagc gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac
    17401 gatcgccgag aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca
    17461 taggcagatc gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac
    17521 cggcatgatg acgtaaacgc tggctacccg gctgcgcacc cagcaatcgg tgcaaagtac
    17581 tggcatcttc cggaatgcgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct
    17641 tgccgagaga ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac
    17701 ggcagcgttc gccgtcggcc atttgaatta acgctgccag taacttcgcc acggtggtcg
    17761 ttttaccggt gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgccg
    17821 ctgcaacttt ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg
    17881 ccagtagagc ttcatcaacc tcaatggcat gattcacctc attgaagaag cgcgctaccg
    17941 tccgctcgtt acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg
    18001 tgggttcatc tcccctgctg accgcttgag aagccagcaa gcattcttcc caattttgta
    18061 gctcaccgat ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca
    18121 gtcgtgaaag cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg
    18181 cgagggtgac ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg
    18241 ggcgtagctg tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc
    18301 ctccagggtc ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg
    18361 ggttgtgtaa atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat
    18421 aacgccgcca aagtgacgat catagtcgta atcagcaatg cgatggcgca gataacgatg
    18481 cagcgccagg gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc
    18541 catagcctgt tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag
    18601 caggtaataa cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg
    18661 tacctgcatg aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag
    18721 tgcatcaagc tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac
    18781 ctgtttatcg cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg
    18841 gaggacagcc gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag
    18901 ttccagtttt tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa
    18961 caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt
    19021 cggttcttca acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc
    19081 ctgagcgata ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc
    19141 gccgggcaat cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg
    19201 ccagggctgg ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc
    19261 ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc
    19321 gagcgcactt tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg
    19381 tgcaacgccg agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag
    19441 atcttccgcc agacgttcga cctccgcgag gtcgacgctt tctggcgcag cgttaagatc
    19501 caaaactgcc tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt
    19561 ggtgataaac ggcagccaga ccaatggata ttccagccct ttcgatttgt ggatcgtgac
    19621 aatctgcacc agatgtttat cactttcgag acgcatttgt tggctggagg cattactgtc
    19681 tggctcgagg atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt
    19741 tccggcttct tgtagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc
    19801 tgccgttgcc agcaagtttt cagcaatgtt acgcgccgac atcagcgccc gcagcatcgg
    19861 cataacgcca cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccacatc
    19921 ccacgcatgt tcgtcattgt tcagcgtttc gatatccagc gcgttcagcc ccatcattga
    19981 cgttgccagc gcactacgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacaa
    20041 ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac
    20101 ggaagggatt tccagcaacg ttaaggcgtc gcgcacctgg gcggcctcct ggcggctgcg
    20161 caccagcaca ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa
    20221 cgcttcgccc cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc
    20281 catggtactt tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat
    20341 cgcaggctgc gtttcacctt taaacacaaa acgtaacgcc tgatttttcc cggctgattt
    20401 cactggaata aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt
    20461 gttcacactg ttcaccattc ctggtgcgga acgccagttg gtgtctaaag tgtagtgggc
    20521 gtgaacttcg ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat
    20581 ggcctgtttc gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt gccagatacg
    20641 gcgaaaaatt cggtactgct gggggtcggt atcctgaaat tcatcaatca ttgccaccgg
    20701 gaatcgcgta cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc
    20761 gagccgactt aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac
    20821 tgtttcgcgg atctcagcca atgcgcgggt tatcaccaga tcacggatcg acaatggttc
    20881 tgcaagcagt tgttcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt
    20941 cgtgcgatct tctaagaaac gctgagagaa tttttccagc gactccggta actgataact
    21001 gttggtctct tcttctgccc aggcgctgat cttctcgatc catttagcct gattgctacg
    21061 gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc
    21121 gtcgcgccac tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt ggcgggaagc
    21181 cagcgtttca tcatcgggcg gcggtgcttt gataaccggt gcttcgcctt gcagataacg
    21241 attaatatcg cgcagcaacg cctgcggccc tttccaggtt tcaaagacca ccagggcaat
    21301 ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag
    21361 cagagactca tcttcaatca gctgctgctc aaacagcata ccggattcaa aggcattcag
    21421 gttgagcatg cgctggcaaa aaccgtgaat agtaaagact gccgcttcat ccatctgccg
    21481 ttcggctaac aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggcg
    21541 ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt
    21601 gctacggata cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag
    21661 ttcttcaacg gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg
    21721 caaatagagc gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg
    21781 ctcgccctgt aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt
    21841 cactcatcag gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt
    21901 caggatgcac atattcggct ttcccgttct ggctgccgga aatctgcgac aggatagcca
    21961 tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc aagtttttgc ggcgtcagca
    22021 gttttatctg ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat
    22081 ctttacttaa cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa
    22141 ttaccgcctg ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg
    22201 cggttgggaa aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt
    22261 gcaaaaggaa gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat
    22321 agcccaattg ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca
    22381 acagagagct ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg
    22441 ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga
    22501 ccactacatc tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat
    22561 cgcgtgccag cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc
    22621 gagcccctga ttttaaggcg tcgcgatagg ccagcacctc tttcaacgta atggagggca
    22681 aaattttacg ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca
    22741 taatcgcctg ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg
    22801 acttcgcctg ctcaagctga tcttccgtag cggtatagct aaagtacccc tcgagcaatg
    22861 cctggaacag ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt
    22921 tagcgttggt ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg
    22981 ccagccctgc gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg
    23041 ctttcggatt acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc
    23101 ttggcgcata caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt
    23161 tcttctctga cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg
    23221 agagcgcaat attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat
    23281 cgacctgata cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga
    23341 tataccagat acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag
    23401 catcgtaccg atcggcaata ttgactgcat ccagcgtatg ctcaaccgga acgcgaatca
    23461 tggtatctgc cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt
    23521 cgatatccag cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta
    23581 acagattgag atagctaaaa attgccgcca caacctgatc gcgattagcc aggcctttat
    23641 cggttaaaga cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt
    23701 tggcgctaat gccctcaact aatccctgct tttgcagcca gtcggaaagt gtacctgggc
    23761 tgcgattacc aatcagatag gtaatcaatt catccgtttt actacggaac ttcgctgagt
    23821 tgttatcgat gcgaaactca acgcgcagaa ctttacgcgg cagtgccggg acgtaatgaa
    23881 tgataatgcc cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct
    23941 ctttgttcgg cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt
    24001 tattgctgta aataaccgcc ttcatcagat tggcggaata gtacttctcg tggaaatctt
    24061 tcagcgcctg ctgcaccgga ttaccaggtt tatcgcttaa agtttcgagg ttaccaccag
    24121 aaaactttga accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca
    24181 tcccgtcacg cgtacgcgcc atggttaatt cagcgttcac cgcattacgc tcacgttcgg
    24241 catatttctt gtcgagcaaa ggttcagcaa tagcatcggc caggcggtct accgcaccag
    24301 gcaaggcgtc gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat
    24361 tgtgactacc gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt
    24421 tcgaccccat cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat
    24481 cttccagcga cccaacgggc accaccagcg ccgagagcga tttaactgcc tgcggatcag
    24541 aaaccagcaa gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt
    24601 tatcactttt acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg
    24661 gtgcccaaag ggcaacaaac aacaataatg ctttgaacca ggtgctgcgg ggcattcacg
    24721 gacctcataa tcaactttat tttctggcca gattcaacag gttaacgcaa atcatctgcc
    24781 agaatttaat cttgtgctgc acgagtcagc ctatgtctat atatccatca gtccgtgact
    24841 ggtgcgcatc ataaagtaag cggatagatt gcgcaatttt tatacagcac tcatgactga
    24901 ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt caacgatggc ctccattgtc
    24961 tctggtgtta attgccgcca gagcctttga taccagatat catcaccttc gccacgcacc
    25021 atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg
    25081 tcatccagca tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt
    25141 tcaggtaaca ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag
    25201 aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt
    25261 agaaaaaggc gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa
    25321 agttgcattc cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc
    25381 tgcacctgcg gcaaccagcc agttatctgc acaccgttgc aggcgagatc aatttccata
    25441 ctctgccccg gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac
    25501 tgtgtttccc agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag
    25561 cggcggaaca agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg
    25621 atttgataac ggctaagccc ttccagaata aatggctcgg tgtctgggat ttcgctgtct
    25681 tcagtacgga agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag
    25741 aatcgttgta gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca
    25801 aattcagaat gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt
    25861 tgtcgttcgc ctggctggta gttttgcgga tcaaacggca tccgggtatg gaggcaagta
    25921 agatgcgctt ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga
    25981 taatgacttt gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca
    26041 ctgttatcct gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt
    26101 aacgcttcca ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt
    26161 ttctggctca tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc
    26221 attcccagca ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg
    26281 ttaaccggtc cggcgagaaa acgctggctg atacgttctt gatccagacg ctgtgccagt
    26341 tcatcacgca atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg
    26401 ataatcgcct gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc
    26461 aggaagaagg cgttgagcat atcgcgacaa accggcaacc actcttccag cggacgctcc
    26521 tgtgccagcc cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc
    26581 agttctgcaa ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc
    26641 gcgctctcca tcgcgtagcc caacaacata cgcgtcaggc caaatcgcca ggtgtgttgt
    26701 ccggtggcgg ggagttccag ctcgcgaacg ttgtcgtcat ctatgcccca acgaatgccg
    26761 gattcgttga cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc
    26821 gccagcaccg gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc
    26881 aacgataaca ggctgataaa cgcttccagt accggatgcg actgccgcgc ccgacggtcg
    26941 gaaatggcgt aaggcaggta acgatccgca ggtgcgctac caaacacagc ctgaataaac
    27001 ggactgtagc tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg
    27061 tcttcttcca gcatcgccag caggcgatcg tgcaaaactt caacttcacg ctgcgggcta
    27121 tggcaaacgt ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta
    27181 cgggaaaact cttcgatgtt cacaccagca acggcacggt tttccagttc cagaatgtca
    27241 gactgaatgt tatgcagcag gttatctggc gtgacatcga caaaggcgtc cagatcctgg
    27301 ctgctctcca ggtcagaaag gagataaatg tagtcgcgcc caagcttacc ccatgaagcc
    27361 agcagcgggt tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg
    27421 ctatcacgaa atagcggcaa ttcgcgatct tcaaaactgt gtcgacgctg acgggtcagc
    27481 agtttcgcca gataagcagg atctttaata tcgccccagt aataacggca ggggttggta
    27541 aacaggagat ggatttcaat atgtttaccc agcgcctgga gcgcctggag ataaacaggc
    27601 ggtaacgcgg aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcatc
    27661 gcggactcca gcgtttcgat aaagcgctga tagagattgg cgcggtgcca acgcggttgt
    27721 ccgagttcat gggtatattc caccagcgcc ttccacaacg gcgcttgcca ggcctgtgct
    27781 tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg ccagccactc cgggcgatag
    27841 accagatact ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg
    27901 tcgctatcgt cagtcagata atgccgcaac agggtaaagt cttcgcgatc cagcaattgc
    27961 ggcagcagag tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg
    28021 atctccggta acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca
    28081 atgtttgccg caataccaaa cttttgcgac agggtcattt gcagccactg cgccataccg
    28141 gtactttgca ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca
    28201 ataaactcca tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata
    28261 gcggctcctg actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc
    28321 ccgggtgaaa caagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg
    28381 ttgacctgcc agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc
    28441 cgccagagct gttggtactg gtttctgctg gcgagactgt tcattaatgt tcgctggata
    28501 cccgataacg cagtgacaat catcaccatc agcaccatcg caaccattac ctccggcagg
    28561 ctaaagcctt gttgattctt cagggaagct gacataacgc cctctctttc agcggacaaa
    28621 aatcgctcca gccgcgtggc gaaaaaacaa tgtttccatc aatgacttcg cctgttcgcc
    28681 acaacgaaac gccttcataa ccggcaatca ataaggcttc attatctgcc agtaaacgca
    28741 aacaaacctg ggcatcggtt ccagcgtact gcgagcactg aactgctggc tgcgtctgcc
    28801 aggagtgcat ttttccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact
    28861 gactttccat gctcacgcga gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata
    28921 gcaagctacc caaaaccagc agcatcagga ccagagccag tgacgaaaca cctttttcgc
    28981 ggttcacagg ttgaatcctg ttacgctata gctggcatcc accacggttt gcggttcaga
    29041 cttactggca gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg
    29101 tacgacctga aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt
    29161 accttcacag gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc
    29221 aatctggtca gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca
    29281 ctgcacaatg atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagctgcc
    29341 atgacaataa cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc
    29401 atcttccagc gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa
    29461 gcgtgcagcc cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag
    29521 agaaaaacct tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt
    29581 cttccccacg gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc
    29641 ccggcccatg cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt
    29701 tcgacttcgg gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc
    29761 ccagcagcgg aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg
    29821 tcgcggttat gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg
    29881 gcggtttgcc atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg
    29941 ctttgcatga ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt
    30001 gttttcatgc cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct
    30061 tcggcgcatc ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg
    30121 tctcgaagaa tttaacggag ggtaaaaaaa accgacgcac actggcgtcg gctctggcag
    30181 gatgtttcgt aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc
    30241 aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa
    30301 cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct
    30361 gtacaggtgc gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg
    30421 cgccatcata tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac
    30481 gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa
    30541 gaacgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac
    30601 aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc
    30661 aatatgacga ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag
    30721 gtcgccgttt tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc
    30781 aatgttagtg tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca
    30841 acgtttagtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa
    30901 aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt
    30961 ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg
    31021 acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg
    31081 tcccatgctg atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa
    31141 ctcaacaatg atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat
    31201 tgggcgcggt ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa
    31261 cagctcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg
    31321 ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat
    31381 ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag
    31441 acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa
    31501 ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc
    31561 gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg
    31621 cgggaaattg tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc
    31681 cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg
    31741 tcgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg
    31801 aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct
    31861 actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat
    31921 gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg
    31981 gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag
    32041 cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag
    32101 ttgcgcttcc agactacgct gcgcaagatt ttctgcgtcg gcataatcaa tgcgccgcag
    32161 caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc
    32221 aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat
    32281 tccatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag
    32341 actgtcataa atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt
    32401 cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa
    32461 taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat
    32521 catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc
    32581 gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag
    32641 catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg
    32701 ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc
    32761 gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata
    32821 ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt
    32881 gcgataaaga ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa
    32941 accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac
    33001 gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg
    33061 cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt
    33121 cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc
    33181 acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa
    33241 gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa
    33301 acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg
    33361 acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa
    33421 ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc
    33481 aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc
    33541 gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa
    33601 ctcgtttgcc gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag
    33661 cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc
    33721 aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa
    33781 caaggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag
    33841 gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc
    33901 aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt
    33961 tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc
    34021 cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc
    34081 gcgtggtttt ttcagccccc gggtcgccat caagtagtaa caacgtcgat catgatcggc
    34141 caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt
    34201 taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt
    34261 gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt
    34321 tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta
    34381 gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca
    34441 gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag
    34501 ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac
    34561 caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc
    34621 tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc
    34681 tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca
    34741 cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc
    34801 atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca
    34861 ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac
    34921 agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc
    34981 tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat
    35041 aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga
    35101 gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag
    35161 atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa
    35221 aaactggagt ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac
    35281 agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag
    35341 atttggaaaa cccgttgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat
    35401 aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa
    35461 ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct
    35521 cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg
    35581 ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc
    35641 tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa
    35701 gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg
    35761 ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt gacatgggaa
    35821 accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc
    35881 agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag
    35941 gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag
    36001 gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat
    36061 gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc
    36121 ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag
    36181 tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca
    36241 gggcttttta gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta
    36301 cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg
    36361 tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca
    36421 tggttatgcg tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc
    36481 tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct
    36541 tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag
    36601 ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata
    36661 ttatctttag cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta
    36721 tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg
    36781 attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg
    36841 gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg
    36901 cgatgttctt ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa aagaatccgc
    36961 tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt
    37021 taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg
    37081 taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact
    37141 ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat
    37201 gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga
    37261 tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta
    37321 acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct
    37381 ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac
    37441 cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt
    37501 gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt
    37561 aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta
    37621 accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc
    37681 gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg
    37741 ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat
    37801 tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc
    37861 ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg
    37921 gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt
    37981 ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat tgtcaccatt
    38041 cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag
    38101 tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa
    38161 tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg gttcacccgc
    38221 tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat
    38281 ggcctggacc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc
    38341 actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg
    38401 gagttaagcg aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg
    38461 gcaccataag attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga
    38521 tccacagcgc cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga
    38581 ccgggatgcc tatcattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt
    38641 tttcgccaag gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct
    38701 cctgtagcaa cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca
    38761 acaaggcata ggctggcagc agctcgtatt gcagggaaaa aatcagtacc accacgccaa
    38821 tcaaaatccc ggctggcata cagcgtgaca cagtttccag cgtaactaac ttcgctgccg
    38881 cacctgcgcc aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagcgt
    38941 tatcggtaat gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac
    39001 ccgctcccca gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata
    39061 gcgaggtgca ggcattcagg aaactgcggg tcatgttgat gagattccag gactgccccg
    39121 gacgcgccgc cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca
    39181 gtgcgcatgc ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg
    39241 aaccgagcaa aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact
    39301 tactacccgt ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac
    39361 caacacccac cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc
    39421 ccagcagctt caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca
    39481 cctgcccgac aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg
    39541 gctggctcca ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg
    39601 cattatcgcc aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc
    39661 acaacgaagt gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag
    39721 cttttcaacg tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag
    39781 cgaatatcgc gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac
    39841 ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca
    39901 tcgctcttaa tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc
    39961 accatttcca gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga
    40021 atctgcacaa agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc
    40081 tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc
    40141 cgcagatagc cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata
    40201 ccagggaccg acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt
    40261 ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc attcggtcac gccgtagcct
    40321 tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtaatttt
    40381 tctgcgcctg ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg
    40441 tagtgaccga ggaaagtcga ggtgccgaac aacacggtgc aactgcggtc atacaccaac
    40501 tccggcacaa tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc
    40561 agtggcgtaa acaggcctac cgtcagccca aaggagtgaa acagcggtaa cgccgacata
    40621 aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct gctcgacatt cgccagaatg
    40681 cttttatggc tatggacgac gcctttcgga tggccttcag aaccggaggt aaaaaggatc
    40741 agcgcctctt cttccggctg ctgtttaacc tgtgccagac gcggcatcag caaatgagcg
    40801 aagatccata ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag
    40861 cgcacctgag taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg
    40921 gaagtgaaga tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttaccccg
    40981 gcagtgtagt tcatcattgc gggcatgcgg cgacgggcga tggccccaaa aatcactgcc
    41041 gcactgatgc ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt
    41101 tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta
    41161 aagttgacgt cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa
    41221 gattcgtaca gcgtttcacg cgggcgcacc gccattcgcg cttccatcat tatttgatgc
    41281 agcatttcgc cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg catcgccacc
    41341 tgcgttggtg gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct
    41401 ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca
    41461 ccagacttcg ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg
    41521 gtgatgcgtc cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga
    41581 tgtttaatag ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc
    41641 caacgcatat accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt
    41701 aaaaacagtc caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg
    41761 cgctcgccct tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac
    41821 aaatttcgaa aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga
    41881 atggagggag attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa
    41941 acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc
    42001 tcaccaatca atacctctgg gatcttgatt gtggtctgca cgacgactct tcgccagcga
    42061 gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg ttactgttac
    42121 agattgatga ccggccaaaa aaaacctgcg catctgcgca ggctggtgta attcatgtgc
    42181 tcaacccgaa gttgacttca cctatcaata cctctgggat caccacttta gcaacctgaa
    42241 gccaaacgcc accagcggtc aatcgcaaca gcatttcgca aagtgtaacc aaaggtttgc
    42301 aatctctttt tttgtcttac ttatttgcgt ttggctcaca ttcccacgat gaaaacacgc
    42361 caccccttga accaacgggc gttttccgta acactgaaag aatgtaagcg tttacccact
    42421 aaggtatttt catggcgacc ataaaggatg tagcccgact ggcaggcgtt tcagtcgcca
    42481 ccgtttcccg cgtcattaat aattcaccta aagccagcga agcttcccgg ctggctgtgc
    42541 atagtgcaat ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg ctggcgcagc
    42601 agaccactga aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt ttcggtgcaa
    42661 tggtgaaagc ggtcgaacag gtggcttatc acaccggtaa tttcttattg attggcaacg
    42721 gttaccacaa cgaacaaaaa gagcgtcagg ctattgagca actgatccgc catcgctgtg
    42781 ctgcgttggt cgtccatgcc aaaatgatcc cggatgccga tttagcctca ttaatgaaac
    42841 aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac cgttgtattg
    42901 ctcttgacga tcgttacggt gcctggctgg caacgcgtca tttaattcag caaggtcata
    42961 cccgcattgg ttatctgtgc tctaaccact ctatttctga cgccgaagat cgtctgcaag
    43021 ggtattacga tgcccttgct gaaagtggta ttgcggccaa tgaccggctg gtgacatttg
    43081 gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga gcttttggga cgaggaagaa
    43141 atttcactgc ggtagcctgt tataacgatt caatggcggc gggtgcgatg ggcgttctca
    43201 atgataatgg tattgatgta ccgggtgaga tttcgttaat tggctttgat gatgtgctgg
    43261 tgtcacgcta tgtgcgcccg cgcctgacca ccgtgcgtta cccaatcgtg acgatggcga
    43321 cccaggctgc cgaactggct ttggcgctgg cggataatcg ccctctcccg gaaattacta
    43381 atgtctttag tccgacgctg gtacgtcgcc attcagtgtc aactccgtcg ctggaggcaa
    43441 gtcatcatgc aaccagcgac taaccgcagt taaagcaatt ccagcgccag taattcttcg
    43501 atggtctggc gacggcgaat caaccgcgcc tgaccattat caaacagaac ttctggtaac
    43561 agcggacggc tgttgtagtt ggatgacatt gatgcgccat atgcccctgt atcatgcagt
    43621 accagataat cacccgcctt cacttccggc aaggcgcggg tttcaacatt tcccccttcc
    43681 tgctgggtaa agacatcgcc cgattcacat aatggcccgg cgacgacggt ttccaccgtt
    43741 ggcgcgtgtt ccagagaacg accatcagct gccagggcac tgatatggtg gtaactaccg
    43801 tacattgccg ggcgcatcag atcgttgaac ccagcatcaa ccagcacaaa gtggcggcta
    43861 cccatttgtt tgacgctccg cacctgagta attaatacgc cagactgcgc taccaggaag
    43921 cgacccggtt caatttccag tttcacaggg tggcccaaat ggcgggcgat ttgctcacgc
    43981 gcggcattcc acagaccata ataatgttcg gtatcaaccg cctcttcacc ctgttgataa
    44041 ggaacagaaa gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa ttcgatgacc
    44101 tgacgcacca tagcaccaca cacctgttcc agatgggcat aatcaacgcc agaaccaatg
    44161 tgcatgtgaa tgccgaccag ctgcagatga tgacgttgta tcacgtccag tgcggcgggc
    44221 agatcggtgt accagatacc gtgcttgctg ttttcgccac cggtattggt tttttggcta
    44281 tgtccgtgac caaaccccgg attaacgcgc agccataccc gatgccctgg cgaaacctgg
    44341 cccagttggt cgagcatatc aacagaaccc gcattcaccg gaatttgcaa ttcactgacg
    44401 cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa caatatcatc ggggtgcgtt
    44461 tgcggattgt aacccgccgc caacgcacgc tctatttcgc ctaacgagac ggaatccact
    44521 ttcacgccct gctcacgcat taagcgcaaa atatgaatat tggaacaggc tttctgtgca
    44581 aagcgcacca catcaaactg tttcagcgct gcaatctgcc gacgaataat ttgcgcatcg
    44641 tagacccaca ccgggcagcc aaattcagcg ggcaaacgca gcagattttc ggcggtgaga
    44701 tcggtatcgg tgctgaacag tgaatgtggc ataacaaact ccagataagt gcttttttat
    44761 gattacgcca catcataaaa agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa
    44821 tgatatggat taccggattg cgagagagcg ctaatggccg ccgttaactt acgtcatatt
    44881 gaaatttttc atgcggtaat gaccgccgga agcctgactg aggcagcaca cctgctacac
    44941 acctcacagc caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat cgggctaaaa
    45001 ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc aaggactgcg tctgtttgaa
    45061 gaagtgcaac gatcctggta cggactggat cgcattgtca gcgccgcaga aagtctgcgc
    45121 gagtttcgcc agggagaact gtctattgcc tgcctgccgg tcttttcgca atctttttta
    45181 ccgcagctcc tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc
    45241 caggaatcac cgctacttga agagtggctc tcggcccagc gtcatgattt aggactcact
    45301 gaaacgctcc atacgcctgc gggaacagaa cgtaccgaat tactctcttt agatgaagtg
    45361 tgtgtgttac ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc ggatgatttt
    45421 cagggtgaga actacatcag cctttcccgt actgacagct atcgccagtt gctggatcag
    45481 ctatttactg aacatcaggt taaacgacgc atgatcgtag aaacccacag cgccgcgtca
    45541 gtctgcgcaa tggtacgggc gggggtaggt gtttcggtgg ttaacccgct caccgcactg
    45601 gattatgcgg caagtggttt agtggtgcgg cggttcagta ttgcggttcc gttcaccgtc
    45661 agcctgatcc gccccctgca ccgcccgtca tcagcgctgg tgcaggcgtt tagtgagcat
    45721 ttacaagcgg ggttaccgaa actggtcact tctcttgacg ctattttgtc gtcagctacg
    45781 acagcataaa agcgacagca tcctcggcat ggatcgccgc agtatcaaac acaggcaaaa
    45841 cactgcgctc ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg acgccctgtg
    45901 cgccctgttc tgcaaggcga gcaatcactt gcgcataata agcgcgtgac gcttcggtaa
    45961 attgccccag acacagttct tcaaaaataa tctgattaat tttcgcccgt tcatccgctt
    46021 caggaataag acaattgatg gaaaattgtt ccatcagccg cccgcgataa aaatcctgtt
    46081 ccatggtgta acgcgtaccc agcagcgcca cacgagtcat tccggccccg gtaattgcac
    46141 gtccggtggc atccgcaatg tgtaagaaag gcagagtgca acgtgactca atggcatccg
    46201 ccactttatg catcgtattg gtacataaca caataccttc tgcgcccgcc cgctgtaagc
    46261 caagcgccgc ctcagccaga atgtccccgg ttttatccca ttccccgcga cgctggcact
    46321 cttctatttc atgaaaatcg acgctatgta gcagcacttg cgcagagtga agcccaccaa
    46381 gccgctgttt aatgccttca tttatcagac gatagtaagg aatggtggat tcccagctca
    46441 ttcctcccag caaaccaatt gttttcattc ctctctcctg atgtgtgtta ccgcaacaag
    46501 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccgatctct attaacgaaa
    46561 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
    46621 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
    46681 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
    46741 ggcagcgcca atgctatcaa gcagtgtcag gaaggtcgcg ccgataatca tattcgacac
    46801 ccagtttgtc gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
    46861 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
    46921 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
    46981 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
    47041 cggtttacga cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga acataaaggt
    47101 cagtcccacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
    47161 gaagatacgc ggcgcatagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
    47221 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
    47281 accgccttgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
    47341 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    47401 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    47461 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    47521 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    47581 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    47641 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    47701 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgacccag
    47761 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    47821 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    47881 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    47941 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    48001 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    48061 acaactcacg caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctacgacat
    48121 gtcgttatgt gatggatatt cc
//
LOCUS       DAZFFI010000025        46389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFFI010000025 DAZFFI010000000
VERSION     DAZFFI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.025"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHJ12_001723"
     CDS             complement(87..1946)
                     /gene="gss"
                     /locus_tag="ACHJ12_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4989521.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQTGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2151..3017
                     /gene="yghU"
                     /locus_tag="ACHJ12_001724"
     CDS             2151..3017
                     /gene="yghU"
                     /locus_tag="ACHJ12_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4989522.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGDG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="ACHJ12_001725"
     CDS             complement(3141..3389)
                     /gene="hybG"
                     /locus_tag="ACHJ12_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4989523.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="ACHJ12_001726"
     CDS             complement(3402..3743)
                     /gene="hypA"
                     /locus_tag="ACHJ12_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4989524.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="ACHJ12_001727"
     CDS             complement(3736..4224)
                     /gene="hybE"
                     /locus_tag="ACHJ12_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4989525.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4217..4711)
                     /locus_tag="ACHJ12_001728"
     CDS             complement(4217..4711)
                     /locus_tag="ACHJ12_001728"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4989526.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="ACHJ12_001729"
     CDS             complement(4711..6414)
                     /gene="hybC"
                     /locus_tag="ACHJ12_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4989527.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="ACHJ12_001730"
     CDS             complement(6411..7589)
                     /gene="hybB"
                     /locus_tag="ACHJ12_001730"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4989528.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="ACHJ12_001731"
     CDS             complement(7579..8565)
                     /gene="hybA"
                     /locus_tag="ACHJ12_001731"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4989529.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="ACHJ12_001732"
     CDS             complement(8568..9686)
                     /gene="hybO"
                     /locus_tag="ACHJ12_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4989530.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9875..10162)
                     /gene="yghW"
                     /locus_tag="ACHJ12_001733"
     CDS             complement(9875..10162)
                     /gene="yghW"
                     /locus_tag="ACHJ12_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4989531.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10281..11168)
                     /gene="yghX"
                     /locus_tag="ACHJ12_001734"
     CDS             complement(10281..11168)
                     /gene="yghX"
                     /locus_tag="ACHJ12_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4989532.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11325..12365
                     /gene="gpr"
                     /locus_tag="ACHJ12_001735"
     CDS             11325..12365
                     /gene="gpr"
                     /locus_tag="ACHJ12_001735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4989533.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12405..12899)
                     /locus_tag="ACHJ12_001736"
     CDS             complement(12405..12899)
                     /locus_tag="ACHJ12_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4989534.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13090..13974
                     /gene="yghA"
                     /locus_tag="ACHJ12_001737"
     CDS             13090..13974
                     /gene="yghA"
                     /locus_tag="ACHJ12_001737"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4989535.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14246..14671)
                     /gene="exbD"
                     /locus_tag="ACHJ12_001738"
     CDS             complement(14246..14671)
                     /gene="exbD"
                     /locus_tag="ACHJ12_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4989536.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14678..15412)
                     /gene="exbB"
                     /locus_tag="ACHJ12_001739"
     CDS             complement(14678..15412)
                     /gene="exbB"
                     /locus_tag="ACHJ12_001739"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4989537.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15664..16851
                     /gene="metC"
                     /locus_tag="ACHJ12_001740"
     CDS             15664..16851
                     /gene="metC"
                     /locus_tag="ACHJ12_001740"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4989538.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDSFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16991..17650
                     /gene="yghB"
                     /locus_tag="ACHJ12_001741"
     CDS             16991..17650
                     /gene="yghB"
                     /locus_tag="ACHJ12_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4989539.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17690..18589)
                     /gene="yqhC"
                     /locus_tag="ACHJ12_001742"
     CDS             complement(17690..18589)
                     /gene="yqhC"
                     /locus_tag="ACHJ12_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4989540.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18783..19946
                     /gene="yqhD"
                     /locus_tag="ACHJ12_001743"
     CDS             18783..19946
                     /gene="yqhD"
                     /locus_tag="ACHJ12_001743"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4989541.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20051..20878
                     /gene="dkgA"
                     /locus_tag="ACHJ12_001744"
     CDS             20051..20878
                     /gene="dkgA"
                     /locus_tag="ACHJ12_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4989542.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21078..22004
                     /locus_tag="ACHJ12_001745"
     CDS             21078..22004
                     /locus_tag="ACHJ12_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO4989543.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22055..22312
                     /gene="yqhH"
                     /locus_tag="ACHJ12_001746"
     CDS             22055..22312
                     /gene="yqhH"
                     /locus_tag="ACHJ12_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4989544.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22355..24574)
                     /locus_tag="ACHJ12_001747"
     CDS             complement(22355..24574)
                     /locus_tag="ACHJ12_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4989545.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24685..26097)
                     /gene="ftsP"
                     /locus_tag="ACHJ12_001748"
     CDS             complement(24685..26097)
                     /gene="ftsP"
                     /locus_tag="ACHJ12_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4989546.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26172..26909)
                     /gene="plsC"
                     /locus_tag="ACHJ12_001749"
                     /gene_synonym="parF"
     CDS             complement(26172..26909)
                     /gene="plsC"
                     /locus_tag="ACHJ12_001749"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4989547.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27142..29400)
                     /gene="parC"
                     /locus_tag="ACHJ12_001750"
     CDS             complement(27142..29400)
                     /gene="parC"
                     /locus_tag="ACHJ12_001750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4989548.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29946..30428)
                     /locus_tag="ACHJ12_001751"
     CDS             complement(29946..30428)
                     /locus_tag="ACHJ12_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4989549.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30481..30873)
                     /gene="ygiW"
                     /locus_tag="ACHJ12_001752"
     CDS             complement(30481..30873)
                     /gene="ygiW"
                     /locus_tag="ACHJ12_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4989550.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31025..31684
                     /gene="qseB"
                     /locus_tag="ACHJ12_001753"
     CDS             31025..31684
                     /gene="qseB"
                     /locus_tag="ACHJ12_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4989551.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31681..33030
                     /gene="qseC"
                     /locus_tag="ACHJ12_001754"
     CDS             31681..33030
                     /gene="qseC"
                     /locus_tag="ACHJ12_001754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4989552.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33076..33408)
                     /locus_tag="ACHJ12_001755"
     CDS             complement(33076..33408)
                     /locus_tag="ACHJ12_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO4989553.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            33727..34308
                     /gene="mdaB"
                     /locus_tag="ACHJ12_001756"
     CDS             33727..34308
                     /gene="mdaB"
                     /locus_tag="ACHJ12_001756"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO4989554.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34339..34653
                     /locus_tag="ACHJ12_001757"
     CDS             34339..34653
                     /locus_tag="ACHJ12_001757"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4989555.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34701..36593)
                     /gene="parE"
                     /locus_tag="ACHJ12_001758"
     CDS             complement(34701..36593)
                     /gene="parE"
                     /locus_tag="ACHJ12_001758"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4989556.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36622..37203)
                     /gene="yqiA"
                     /locus_tag="ACHJ12_001759"
     CDS             complement(36622..37203)
                     /gene="yqiA"
                     /locus_tag="ACHJ12_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4989557.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37203..38030)
                     /gene="cpdA"
                     /locus_tag="ACHJ12_001760"
     CDS             complement(37203..38030)
                     /gene="cpdA"
                     /locus_tag="ACHJ12_001760"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4989558.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38055..38477)
                     /locus_tag="ACHJ12_001761"
     CDS             complement(38055..38477)
                     /locus_tag="ACHJ12_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4989559.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38478..39107)
                     /gene="nudF"
                     /locus_tag="ACHJ12_001762"
     CDS             complement(38478..39107)
                     /gene="nudF"
                     /locus_tag="ACHJ12_001762"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO4989560.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39312..40793
                     /gene="tolC"
                     /locus_tag="ACHJ12_001763"
     CDS             39312..40793
                     /gene="tolC"
                     /locus_tag="ACHJ12_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4989561.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40941..41612
                     /locus_tag="ACHJ12_001764"
     CDS             40941..41612
                     /locus_tag="ACHJ12_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4989562.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLIAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41618..42778
                     /locus_tag="ACHJ12_001765"
     CDS             41618..42778
                     /locus_tag="ACHJ12_001765"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4989563.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42816..43604)
                     /gene="ygiD"
                     /locus_tag="ACHJ12_001766"
     CDS             complement(42816..43604)
                     /gene="ygiD"
                     /locus_tag="ACHJ12_001766"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4989564.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPMLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43747..44520
                     /gene="zupT"
                     /locus_tag="ACHJ12_001767"
     CDS             43747..44520
                     /gene="zupT"
                     /locus_tag="ACHJ12_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4989565.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44578..44748)
                     /gene="yqiD"
                     /locus_tag="ACHJ12_001768"
     CDS             complement(44578..44748)
                     /gene="yqiD"
                     /locus_tag="ACHJ12_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4989566.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(45009..45662)
                     /gene="ribB"
                     /locus_tag="ACHJ12_001769"
     CDS             complement(45009..45662)
                     /gene="ribB"
                     /locus_tag="ACHJ12_001769"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4989567.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45766..45914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46036..46326
                     /gene="ubiK"
                     /locus_tag="ACHJ12_001770"
     CDS             46036..46326
                     /gene="ubiK"
                     /locus_tag="ACHJ12_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4989568.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11481 a        12395 c        11609 g        10904 t
ORIGIN      
        1 tattggaata tataaatatt gaatattttg attaatcccc gtactgatta ttcttcataa
       61 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
      121 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      181 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      241 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      301 tgatggctga cgaggtcgat attgctgcca cagcgaccgg cgatcggttt cactgcgtaa
      361 cctgttttca ccagttcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      421 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      481 ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      541 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      601 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg tccttcccca
      661 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      721 tcaaagcccg tctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      781 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      841 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      901 caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc gttgtactcg
      961 taaaccttca gaccacgctc atccatgcag aaatccatac gaccggtgat catatggtga
     1021 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1081 gctagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1141 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1201 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1261 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1321 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1381 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1441 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1501 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1561 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1621 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1681 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1741 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1801 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1861 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1921 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1981 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     2041 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2101 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2161 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2221 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2281 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2341 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2401 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2461 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2521 atctggctga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2581 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcgatggt tttggtcact
     2641 tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2701 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt gcgggcgatg
     2761 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caacgtggtg ttaggtggtg
     2821 tgtatgatgc cgctgagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2881 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2941 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     3001 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3061 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
     3121 gtatctacga aagctaatca tcatgcgctg gtaatgtcgt aatccatttg gcgcagtgcg
     3181 tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc
     3241 agcacccact ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcaca
     3301 tcgcgcttga taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc
     3361 agcacctggc ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt
     3421 ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac
     3481 tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg
     3541 ggtttataga cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca
     3601 aaactaaaac ggacggcact ctcctcaacg caggagagcg cgccaatttc cagccacacg
     3661 gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc
     3721 tggcaaagcg acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg
     3781 ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc
     3841 atccgtcagg cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga
     3901 acaggagagg tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata
     3961 cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc
     4021 ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc
     4081 gaacagcgta aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg
     4141 catcgaacgc cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg
     4201 gaaacctgct atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt
     4261 cgcgcagcgc agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg
     4321 ttaagccgat gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg
     4381 gaaactcgcc ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag
     4441 agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg
     4501 gcgcgctctt cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt
     4561 cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa
     4621 tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa
     4681 tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt
     4741 gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt
     4801 acgcaccact tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc
     4861 gtaaggaccg acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac
     4921 tgcctggtag ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg
     4981 cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac
     5041 aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag
     5101 gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg
     5161 cagctgtgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt
     5221 cagtttgttc tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg
     5281 ccccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc
     5341 gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc
     5401 gtctttatac caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga
     5461 atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa
     5521 caggaagctg ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc
     5581 acctttaccg cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt
     5641 ataaacctgc tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg
     5701 ctcaaggttc aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc
     5761 caggttctga atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg
     5821 ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag
     5881 tttcatcgct gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac
     5941 cagatctttg atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt
     6001 cgaaacgcct ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat
     6061 gtccacccag tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg
     6121 cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc
     6181 actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg
     6241 ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg
     6301 ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat
     6361 gcgtaaatgc ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg
     6421 ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga
     6481 atgagtacga tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct
     6541 tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc
     6601 agacgccagg ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa
     6661 tcattacgca gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca
     6721 atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag
     6781 ataagctcgc caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc
     6841 agcttaacaa acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc
     6901 gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc
     6961 ggcaacattt catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc
     7021 gaccccattg aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac
     7081 atcaccttgt ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga
     7141 gcaaactcca gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt
     7201 accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc
     7261 acgtcgatag tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc
     7321 ggacgcacca gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc
     7381 cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac
     7441 gggaagccgc cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc
     7501 aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg
     7561 ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt
     7621 ggtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc
     7681 tttatacagg gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt
     7741 cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc
     7801 ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc
     7861 agatttcagc gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt
     7921 tttcgcctcc gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac
     7981 ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag
     8041 ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg
     8101 gcaggcgacc atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc
     8161 ggttttcgga tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac
     8221 gcagtgcata cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac
     8281 ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt
     8341 gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt
     8401 ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc
     8461 cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag
     8521 caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt
     8581 gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca
     8641 ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg
     8701 ccctctttag cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca
     8761 agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga
     8821 ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg
     8881 taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca
     8941 tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg
     9001 tgaatcagac ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg
     9061 ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc
     9121 ggaatattga taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg
     9181 ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct
     9241 gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa
     9301 taaataccgt tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg
     9361 tacttctcaa gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc
     9421 acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt
     9481 gcacgaagca gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc
     9541 ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc
     9601 atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa
     9661 tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata
     9721 ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta
     9781 tggttatcga tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg
     9841 taattaatta aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca
     9901 taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt
     9961 tatccagtcc atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc
    10021 cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac
    10081 agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc
    10141 ctttaccaaa atggttattc atcgttcacc tcattcgtta tatatcgcca tatatagcga
    10201 tattttaggc aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa
    10261 tccagtaaga gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc
    10321 caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga
    10381 ttaacccccg gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg
    10441 taagcaggcc agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc
    10501 gcttcaatct tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag
    10561 gccagttccg gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg
    10621 gtaataccca ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag
    10681 aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca
    10741 tttcccggat aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc
    10801 ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc
    10861 actaccacgg ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc
    10921 tcgccgtgac catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta
    10981 aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc
    11041 gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc
    11101 ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa
    11161 cgcggcatcg ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt
    11221 tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa
    11281 actagaaaca ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga
    11341 atcccgaacg ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc
    11401 ccgcgttatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc
    11461 gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt
    11521 acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg
    11581 ccgcttatcg cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc
    11641 cttacggctc tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc
    11701 gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga
    11761 tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg
    11821 ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga
    11881 aaattccgct tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa
    11941 gcggcctgct ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg
    12001 ctcagggatt gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc
    12061 gtgaagggaa taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca
    12121 gcctgcgctt attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt
    12181 taagttggtt gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg
    12241 agcaacttga ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg
    12301 cgcagattga tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata
    12361 aatgacctgt taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga
    12421 gtcagtcggt caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg
    12481 acgtaccaca tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg
    12541 tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc
    12601 attttcccca gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa
    12661 ttctcataac cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc
    12721 agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg
    12781 tgaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag
    12841 tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata
    12901 cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca
    12961 acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc
    13021 ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg
    13081 agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc
    13141 aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc
    13201 gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc
    13261 gattccggaa taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg
    13321 atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa
    13381 tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg
    13441 ctggttcacg aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg
    13501 aaacaggttg ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt
    13561 gccattaacg ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa
    13621 ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg
    13681 gactatgcgg ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc
    13741 gcggagaaag gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa
    13801 atttccggcg gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag
    13861 cgtgcggggc aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg
    13921 agctacgtca ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat
    13981 gcccggttgt gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa
    14041 aacgccttat ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc
    14101 ctgataagcg tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt
    14161 atccggccta caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga
    14221 gcgtagcgca tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac
    14281 ctatcttcag gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat
    14341 cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat
    14401 tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt
    14461 ctgccttcac cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag
    14521 caggcaagtt caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca
    14581 gcagaaccaa catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat
    14641 cgtcgaggtt ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg
    14701 cgacacgaac cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca
    14761 acaatacctg cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa
    14821 atacgttata gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca
    14881 gagcttctgc gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac
    14941 caataaagct gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg
    15001 cgccgatggt tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc
    15061 gacgctccag acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg
    15121 acagttccag ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa
    15181 aatcagcggc gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct
    15241 cgcgcttaag gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc
    15301 aagtgactac ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt
    15361 gctgatacat accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc
    15421 tccaggacgc aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag
    15481 tcgttctcat tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc
    15541 agtaaaaaag tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag
    15601 gatgcgcccg cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc
    15661 gacatggcgg acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac
    15721 actctcggcg cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg
    15781 gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc
    15841 ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc
    15901 tgcgcgctat ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa
    15961 cagggcgatc atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc
    16021 aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat
    16081 atcgttaagc atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc
    16141 accatggaag tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat
    16201 gccatcatta tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt
    16261 ggcatcgatg tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg
    16321 attggcactg ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg
    16381 atgggccaga tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta
    16441 ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa
    16501 catccgcaag ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc
    16561 tggaaacgag actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc
    16621 agtaatgaag agctggcgaa ctatctggat agcttcagtt tattcagcat ggcctactcg
    16681 tggggcgggt atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc
    16741 ccacaaggcg agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat
    16801 gtcgacgatc tgattgccga tctggacgcc ggttttgcgc ggattgtata acattgccac
    16861 ttttggacaa ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc
    16921 aaggcgtccc ggacgattca ggagtacaat aggcaaataa aggcttaaac gctgttccac
    16981 aggaaagtcc atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc
    17041 cgcgctggcg gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt
    17101 tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc
    17161 aggcgcattg attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc
    17221 cgcagcaagt ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa
    17281 aacggtgaaa ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat
    17341 gtttgaccgc cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac
    17401 gctgctgcca accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa
    17461 ctggttaagc ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat
    17521 gattccgttc gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat
    17581 tgccttgtta accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg
    17641 taacgcctga cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct
    17701 gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact
    17761 cacggctaaa ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca
    17821 tgccgtcatg gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca
    17881 acggcgaggt actggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg
    17941 cttctgccgc cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca
    18001 gcacgcggct aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc
    18061 cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac
    18121 gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt
    18181 ctgataacgt ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt
    18241 cgccaatgtc catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg
    18301 ccagcggcac ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca
    18361 gcaggtattc attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc
    18421 cggaaaagag aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct
    18481 cgccatacaa caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca
    18541 gtttattaac tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt
    18601 tcttaggaat aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct
    18661 ggagaaatag gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac
    18721 aatgaaaaac atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag
    18781 taatgaacaa ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg
    18841 ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca
    18901 gcgtgaaaaa aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc
    18961 tggaatttgg cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac
    19021 tggttcgcga acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg
    19081 gcaccaaatt tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc
    19141 tgcaaacggg cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc
    19201 cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca
    19261 agcaggcgtt ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata
    19321 cctacaccct gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg
    19381 tggaacagta tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca
    19441 ttttgctgac gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg
    19501 tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg
    19561 taccgcagga ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg
    19621 atcacgcgca aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca
    19681 agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg
    19741 atgagcgtat tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc
    19801 cgacccacct ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac
    19861 tggaagagca cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc
    19921 gccgtatata cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat
    19981 ttcgtccaga cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa
    20041 ggaggaacgt atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca
    20101 gctgggactg ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc
    20161 gttagaagtg ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt
    20221 cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct
    20281 gtggaacgac gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca
    20341 gcttgattat atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt
    20401 cgaagcatgg aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt
    20461 gtgcaacttc cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt
    20521 gataaaccag atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc
    20581 gacacacaaa atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt
    20641 cgatcagaaa gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt
    20701 tatccgctgg catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg
    20761 tattgccgaa aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat
    20821 tgcaaaactc gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca
    20881 tgcaaattct cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc
    20941 ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc
    21001 atcatgcaga gctggaagct cttaaaagaa caactcatct atatccggga taaacgcaac
    21061 ggagaggtga aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta
    21121 ccgtacacgc acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga
    21181 actataaatc agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt
    21241 ctgcgcgtat tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg
    21301 gctggctgga ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa
    21361 gcgttgcgat aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct
    21421 ttaaagatga taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt
    21481 gggtcattga cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga
    21541 atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg
    21601 ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
    21661 cttaccgcca ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg
    21721 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
    21781 agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc
    21841 gcattcatgt tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac
    21901 agcggcgcga aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
    21961 ttaagcagat tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca
    22021 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
    22081 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct
    22141 ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
    22201 actacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
    22261 tgctcaggac tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
    22321 tccccggcag catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc
    22381 gtttcttcgc accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag
    22441 caggcgtctg acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc
    22501 ggttctggcg acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc
    22561 gacggctgcc aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc
    22621 agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct
    22681 tcggtacgaa gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt
    22741 aatacatggt ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac
    22801 ggaaacgatg ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg
    22861 ggtgcgcgga gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag
    22921 tatcgaacag ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata
    22981 acggcccttc ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg
    23041 ccaactcttt gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga
    23101 tgaggatttt tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt
    23161 cgtggttagt gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag
    23221 tttgttcagc gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga
    23281 gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa
    23341 tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga
    23401 aagaacagcc gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag
    23461 ccggaatacg ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa
    23521 cgctgtccat ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt
    23581 caccgtgttt ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc
    23641 gcgaagcatg ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca
    23701 cgtaggtttt ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct
    23761 gctttttcgg tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg
    23821 ggattgggtc gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag
    23881 gcagcgcctc tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct
    23941 cgcccatcgc cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca
    24001 gcatgtcggc tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat
    24061 aatgtgcggt acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc
    24121 acgcctcttt acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga
    24181 cgttatccgg cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga
    24241 tcatcgaatc catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca
    24301 tgaaatcgtc tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg
    24361 cttccagcat acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc
    24421 cagtaaccaa aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca
    24481 tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt
    24541 cgcgatccgg ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat
    24601 aatcaaaggc cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc
    24661 gccctcctcg ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga
    24721 gccacggtcc gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga
    24781 aggctgaccg aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca
    24841 gcctctgtct tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc
    24901 ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg
    24961 ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc
    25021 acccagactg atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa
    25081 gcgcatcgga agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac
    25141 cagggtagaa accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct
    25201 cgccgcttcg ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc
    25261 gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc
    25321 ctgatcgcca gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg
    25381 agagttcgac gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg
    25441 gctttgtaca ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt
    25501 gtattctggc gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc
    25561 cacaccataa tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat
    25621 tccggcaagg ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca
    25681 cagagtagct gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat
    25741 catccgtgcc ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga
    25801 gacattttct gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg
    25861 gatagtcggc cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt
    25921 aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag
    25981 tagcggcgga acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc
    26041 gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt
    26101 tgctccatgt aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac
    26161 agattgccga cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg
    26221 gcgatttttt gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg
    26281 ccatactgac tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt
    26341 cgattaagat taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc
    26401 gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg
    26461 cgggttcctt ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact
    26521 tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt
    26581 aaccagtaca actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc
    26641 gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg
    26701 atatagatag cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg
    26761 ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta
    26821 cgcgggctga aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg
    26881 gtaataataa gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat
    26941 tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat
    27001 tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg
    27061 ttgaatacgc tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg
    27121 aaacaggccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    27181 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    27241 ttcgccagtg actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg
    27301 aatggtcagc gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg
    27361 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    27421 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    27481 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    27541 agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc
    27601 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    27661 cgccccaggc ggcaacgtta atttgccggt gagcggctcg ccctgaccac gcgccgacgg
    27721 cagcgtaatc gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    27781 gttgctctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    27841 cgcgtcgata tcatggcctt tagcgctgcg tacccaaccc atctgcgaca gcacaatggt
    27901 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    27961 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    28021 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    28081 cagctcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    28141 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    28201 tttcggttca tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac
    28261 cagcaaacct tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    28321 gcggcgcacg gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt
    28381 caccgccgga cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag
    28441 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    28501 cacaatcacc agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    28561 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    28621 ctgatgcggt aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    28681 acgcactgaa ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat
    28741 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt
    28801 tttcggctgg tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg
    28861 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    28921 acgagcaggc agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc
    28981 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    29041 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    29101 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    29161 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    29221 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    29281 aggtttaaga ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    29341 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    29401 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    29461 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    29521 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg
    29581 gcatagaggg cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc
    29641 gggcgattcg agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc
    29701 gactgtaagt atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc
    29761 tgccataagt gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat
    29821 aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc
    29881 ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt
    29941 ccccatcacg ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg
    30001 gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc
    30061 agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg
    30121 cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg
    30181 ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg
    30241 gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc
    30301 tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg
    30361 tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg
    30421 tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa
    30481 ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc
    30541 gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt
    30601 gtggtcgata tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga
    30661 gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc
    30721 gctttctacg gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt
    30781 tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc
    30841 cattactgcg attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt
    30901 ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg
    30961 ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga
    31021 aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg
    31081 ccttagtaaa atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc
    31141 gctttatagc gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg
    31201 tcgcgatatt ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac
    31261 cgcgcgcgat gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct
    31321 gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac
    31381 caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg
    31441 tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga
    31501 attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta
    31561 tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg
    31621 caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa
    31681 atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg
    31741 gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc
    31801 gatgaattgt tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc
    31861 aacgaaatca acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat
    31921 gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac
    31981 gatggcgata acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg
    32041 caactggtgg gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa
    32101 tatcgcatcg ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt
    32161 gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta
    32221 ctattgggtc gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac
    32281 cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt
    32341 gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt
    32401 acctccgacg cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa
    32461 gttgcgcagc tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat
    32521 tacgggatcg atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac
    32581 tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct ccaatcgtcg
    32641 gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat
    32701 gcccacggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg
    32761 ctggataacg ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct
    32821 gataatttca tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt
    32881 ggcgaacgct tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg
    32941 attgtccagc gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa
    33001 ggtggatttg aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga
    33061 ctctcataga ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca
    33121 aaccagacaa ataccgagcg aaaatacata tacccaaaac tcgaacattt cccgcataaa
    33181 gagttttctt aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc
    33241 ttgcaatata cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc
    33301 aagcatccag tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat
    33361 tatgtataac agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc
    33421 tcataaaaat ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat
    33481 tcaaagccat attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc
    33541 gagtaagaat tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc
    33601 atcggtgagt cagagcaaga tcagactttt gctaaatttg caaaagactt tgcacatttt
    33661 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    33721 taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    33781 caactgaacg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    33841 gtccgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    33901 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    33961 aaaaaatata ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc
    34021 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    34081 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    34141 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    34201 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    34261 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    34321 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    34381 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    34441 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    34501 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg
    34561 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag
    34621 atgaatatcc gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg
    34681 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    34741 cagttgcggc gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta
    34801 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    34861 gtggtttcac gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    34921 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    34981 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    35041 tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca
    35101 atgtgcagac catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg
    35161 ctcagatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc
    35221 gaggccagca cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc
    35281 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    35341 tccacaagga acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca
    35401 ggcagtgctg ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg
    35461 cgctgggcgc tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc
    35521 agccacagga taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa
    35581 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca
    35641 taggcgcagc gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg
    35701 cggtattcac agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg
    35761 gtaccgccct gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct
    35821 tccggcagcc acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata
    35881 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    35941 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    36001 acgccggggc acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa
    36061 cgcgggctgt caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt
    36121 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    36181 ttataaacct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    36241 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    36301 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    36361 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag
    36421 attacatcca cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact
    36481 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    36541 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    36601 gttagcaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    36661 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    36721 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    36781 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat
    36841 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    36901 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    36961 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    37021 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    37081 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    37141 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    37201 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    37261 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatagta
    37321 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    37381 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    37441 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    37501 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    37561 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    37621 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    37681 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    37741 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    37801 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    37861 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    37921 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    37981 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    38041 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    38101 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    38161 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    38221 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    38281 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc
    38341 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    38401 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    38461 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc
    38521 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    38581 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    38641 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    38701 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc
    38761 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    38821 gcaaccatct ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat ccgaatctgc
    38881 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    38941 cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc cgttgaatag acgatgacga
    39001 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    39061 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    39121 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    39181 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    39241 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    39301 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    39361 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    39421 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    39481 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    39541 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    39601 tttttgatat gtcgaaatgg cgtgcgttaa cgctacagga aaaagcagca gggattcagg
    39661 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    39721 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    39781 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgata accgacgtgc
    39841 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    39901 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag
    39961 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    40021 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    40081 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    40141 ggatttctga cacctcttat agcggttcga aaacccgtgg tgccgctggt acccagtatg
    40201 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    40261 gcggaatggt taactcgcag gtgaaacagg cacagtacaa ctttgtcggt gccagcgagc
    40321 aactggaaag cgcccatcgt agcgtcgtgc agaccgtgcg ttcctccttc aacaacatta
    40381 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    40441 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    40501 cgaccaccac gctgtacaac gccaagcaag agctggcgaa tgcgcgttat aactacctga
    40561 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    40621 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    40681 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    40741 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    40801 gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca
    40861 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    40921 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    40981 aaaactggag cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    41041 tggctggctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    41101 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    41161 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    41221 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    41281 cccagcaatc cagcgggagt ttctggatgc cgctgatagc cggttacatg atggggcgtc
    41341 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    41401 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    41461 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg
    41521 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    41581 ctggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    41641 gcgcccggac tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg
    41701 cgagccgtac tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct
    41761 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    41821 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca
    41881 gtcatggctg acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac
    41941 tggcgaacct aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc
    42001 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    42061 cgaccagttt aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca
    42121 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    42181 cattcagtat ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat
    42241 cgatgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    42301 caacctgttc aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct
    42361 ggaggatgca ggcgtacgct ggctggaacc agcgtggaag agcattatct ccaacaaggc
    42421 acttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    42481 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    42541 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    42601 tggcgaagaa gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta
    42661 tatgctgatt ggtagctggc tggtgaacga tcaacctgcc ggaattggca ttcgtgaaga
    42721 ccgtgcgttg atcacccagg atatgtctcg gttttatcca catattttta ttgagtaatc
    42781 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    42841 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    42901 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    42961 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    43021 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    43081 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    43141 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    43201 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    43261 caggaaccgt ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggcgctaac
    43321 agctcaacca gatgctgtgc cagcataggc gaacccggag caggataatg cgtatcgtac
    43381 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    43441 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    43501 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    43561 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    43621 aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    43681 caatgatgat catcatcagt tattttgacg atctgcctga aagtgaagat ttataaggag
    43741 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    43801 gcgtttctcg gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga
    43861 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    43921 gctgaaggaa tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat
    43981 tttggcctgg accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg
    44041 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    44101 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    44161 tttggcatcg cactggccgt cgctttgcac aatatccctg aaggtctggc agtggcaggc
    44221 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    44281 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    44341 gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat
    44401 gaattaatgc cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta
    44461 tgtggaatgt cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa
    44521 aaacaacatc agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta
    44581 ttctaccttt tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag
    44641 cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc
    44701 caccacaacc agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt
    44761 aatatttaac aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat
    44821 tcatatggtc gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc
    44881 ctgatattaa tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcggg
    44941 gcaaaataaa aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc
    45001 agcggttttc agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata
    45061 gtcacgagcg ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc
    45121 gtgccatcgt cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc
    45181 atcaaatcaa tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg
    45241 cgaagtggga aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc
    45301 gctgcgcgaa cggtcgtaat acgatcagcg gcagaaacac cggtagtcac accttcagcc
    45361 gcttcaatgg tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt
    45421 ggcagatcaa gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg
    45481 tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg
    45541 ccttcgtttt cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca
    45601 gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc
    45661 atggtaaaaa aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc
    45721 ggcatatagc caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca
    45781 tccggactct aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac
    45841 ccaaagcgct cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg
    45901 ccctgagaat aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc
    45961 acataattct ggtttatgac ttacccttat cgcactacaa tggtactcaa cacctatcag
    46021 tacagggaaa ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac
    46081 gaatcaatgc ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa
    46141 accctacaag ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa
    46201 acgcaagtcc tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatgagcgaa
    46261 ctggaaaatc gcagtactga gattaaaaag cagccagatc cagagactct ccccccaacg
    46321 ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt aatcctctaa tttacagtgc
    46381 tttctatta
//
LOCUS       DAZFFI010000026        45351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFFI010000026 DAZFFI010000000
VERSION     DAZFFI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.026"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..1488
                     /gene="yajR"
                     /locus_tag="ACHJ12_001771"
     CDS             124..1488
                     /gene="yajR"
                     /locus_tag="ACHJ12_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989569.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHJ12_001772"
     CDS             complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHJ12_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4989570.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2275..3186
                     /gene="panE"
                     /locus_tag="ACHJ12_001773"
     CDS             2275..3186
                     /gene="panE"
                     /locus_tag="ACHJ12_001773"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4989571.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3149..3739
                     /gene="yajL"
                     /locus_tag="ACHJ12_001774"
     CDS             3149..3739
                     /gene="yajL"
                     /locus_tag="ACHJ12_001774"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4989572.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHJ12_001775"
     CDS             complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHJ12_001775"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4989573.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5447..5689
                     /gene="xseB"
                     /locus_tag="ACHJ12_001776"
     CDS             5447..5689
                     /gene="xseB"
                     /locus_tag="ACHJ12_001776"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4989574.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5689..6588
                     /gene="ispA"
                     /locus_tag="ACHJ12_001777"
     CDS             5689..6588
                     /gene="ispA"
                     /locus_tag="ACHJ12_001777"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4989575.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6613..8475
                     /gene="dxs"
                     /locus_tag="ACHJ12_001778"
     CDS             6613..8475
                     /gene="dxs"
                     /locus_tag="ACHJ12_001778"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4989576.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8655..9629
                     /gene="yajO"
                     /locus_tag="ACHJ12_001779"
     CDS             8655..9629
                     /gene="yajO"
                     /locus_tag="ACHJ12_001779"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4989577.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(9683..10201)
                     /gene="pgpA"
                     /locus_tag="ACHJ12_001780"
     CDS             complement(9683..10201)
                     /gene="pgpA"
                     /locus_tag="ACHJ12_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4989578.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10179..11156)
                     /gene="thiL"
                     /locus_tag="ACHJ12_001781"
     CDS             complement(10179..11156)
                     /gene="thiL"
                     /locus_tag="ACHJ12_001781"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4989579.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11234..11653)
                     /gene="nusB"
                     /locus_tag="ACHJ12_001782"
     CDS             complement(11234..11653)
                     /gene="nusB"
                     /locus_tag="ACHJ12_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4989580.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11673..12143)
                     /gene="ribE"
                     /locus_tag="ACHJ12_001783"
     CDS             complement(11673..12143)
                     /gene="ribE"
                     /locus_tag="ACHJ12_001783"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4989581.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12232..13335)
                     /gene="ribD"
                     /locus_tag="ACHJ12_001784"
     CDS             complement(12232..13335)
                     /gene="ribD"
                     /locus_tag="ACHJ12_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4989582.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVMPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13339..13788)
                     /gene="nrdR"
                     /locus_tag="ACHJ12_001785"
     CDS             complement(13339..13788)
                     /gene="nrdR"
                     /locus_tag="ACHJ12_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4989583.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13939..14478
                     /gene="yajI"
                     /locus_tag="ACHJ12_001786"
     CDS             13939..14478
                     /gene="yajI"
                     /locus_tag="ACHJ12_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4989584.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14777..15661
                     /gene="tsx"
                     /locus_tag="ACHJ12_001787"
     CDS             14777..15661
                     /gene="tsx"
                     /locus_tag="ACHJ12_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4989585.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15839..16186)
                     /gene="yajD"
                     /locus_tag="ACHJ12_001788"
     CDS             complement(15839..16186)
                     /gene="yajD"
                     /locus_tag="ACHJ12_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4989586.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16315..17286)
                     /gene="secF"
                     /locus_tag="ACHJ12_001789"
     CDS             complement(16315..17286)
                     /gene="secF"
                     /locus_tag="ACHJ12_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4989587.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17297..19144)
                     /gene="secD"
                     /locus_tag="ACHJ12_001790"
     CDS             complement(17297..19144)
                     /gene="secD"
                     /locus_tag="ACHJ12_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4989588.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19172..19504)
                     /gene="yajC"
                     /locus_tag="ACHJ12_001791"
     CDS             complement(19172..19504)
                     /gene="yajC"
                     /locus_tag="ACHJ12_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4989589.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19527..20654)
                     /gene="tgt"
                     /locus_tag="ACHJ12_001792"
     CDS             complement(19527..20654)
                     /gene="tgt"
                     /locus_tag="ACHJ12_001792"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4989590.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20710..21780)
                     /gene="queA"
                     /locus_tag="ACHJ12_001793"
     CDS             complement(20710..21780)
                     /gene="queA"
                     /locus_tag="ACHJ12_001793"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4989591.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21873..22454
                     /gene="acpH"
                     /locus_tag="ACHJ12_001794"
     CDS             21873..22454
                     /gene="acpH"
                     /locus_tag="ACHJ12_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4989592.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22459..24276)
                     /gene="malZ"
                     /locus_tag="ACHJ12_001795"
     CDS             complement(22459..24276)
                     /gene="malZ"
                     /locus_tag="ACHJ12_001795"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4989593.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24432..25805)
                     /gene="proY"
                     /locus_tag="ACHJ12_001796"
     CDS             complement(24432..25805)
                     /gene="proY"
                     /locus_tag="ACHJ12_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO4989594.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25881..27200)
                     /gene="brnQ"
                     /locus_tag="ACHJ12_001797"
     CDS             complement(25881..27200)
                     /gene="brnQ"
                     /locus_tag="ACHJ12_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4989595.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27608..28903)
                     /gene="phoR"
                     /locus_tag="ACHJ12_001798"
     CDS             complement(27608..28903)
                     /gene="phoR"
                     /locus_tag="ACHJ12_001798"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4989596.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28961..29650)
                     /gene="phoB"
                     /locus_tag="ACHJ12_001799"
     CDS             complement(28961..29650)
                     /gene="phoB"
                     /locus_tag="ACHJ12_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4989597.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29840..31042
                     /gene="sbcD"
                     /locus_tag="ACHJ12_001800"
     CDS             29840..31042
                     /gene="sbcD"
                     /locus_tag="ACHJ12_001800"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4989598.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31039..34182
                     /gene="sbcC"
                     /locus_tag="ACHJ12_001801"
     CDS             31039..34182
                     /gene="sbcC"
                     /locus_tag="ACHJ12_001801"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4989599.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34308..35492
                     /gene="araJ"
                     /locus_tag="ACHJ12_001802"
     CDS             34308..35492
                     /gene="araJ"
                     /locus_tag="ACHJ12_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4989600.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(35635..36543)
                     /gene="mak"
                     /locus_tag="ACHJ12_001803"
     CDS             complement(35635..36543)
                     /gene="mak"
                     /locus_tag="ACHJ12_001803"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4989601.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36668..37579
                     /gene="rdgC"
                     /locus_tag="ACHJ12_001804"
     CDS             36668..37579
                     /gene="rdgC"
                     /locus_tag="ACHJ12_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4989602.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37657..37740)
                     /locus_tag="ACHJ12_001805"
     CDS             complement(37657..37740)
                     /locus_tag="ACHJ12_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989603.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37737..38078)
                     /locus_tag="ACHJ12_001806"
                     /pseudo
     CDS             complement(37737..38078)
                     /locus_tag="ACHJ12_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38226..38510)
                     /gene="ppnP"
                     /locus_tag="ACHJ12_001807"
     CDS             complement(38226..38510)
                     /gene="ppnP"
                     /locus_tag="ACHJ12_001807"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4989604.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38582..39259)
                     /gene="aroM"
                     /locus_tag="ACHJ12_001808"
     CDS             complement(38582..39259)
                     /gene="aroM"
                     /locus_tag="ACHJ12_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4989605.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39517..39708)
                     /gene="yaiA"
                     /locus_tag="ACHJ12_001809"
     CDS             complement(39517..39708)
                     /gene="yaiA"
                     /locus_tag="ACHJ12_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4989606.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39758..40282)
                     /gene="aroL"
                     /locus_tag="ACHJ12_001810"
     CDS             complement(39758..40282)
                     /gene="aroL"
                     /locus_tag="ACHJ12_001810"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4989607.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40465..40923)
                     /gene="yaiI"
                     /locus_tag="ACHJ12_001811"
     CDS             complement(40465..40923)
                     /gene="yaiI"
                     /locus_tag="ACHJ12_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4989608.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41043..41852
                     /gene="proC"
                     /locus_tag="ACHJ12_001812"
     CDS             41043..41852
                     /gene="proC"
                     /locus_tag="ACHJ12_001812"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4989609.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41869..42984)
                     /gene="adrA"
                     /locus_tag="ACHJ12_001813"
                     /gene_synonym="dgcC"
     CDS             complement(41869..42984)
                     /gene="adrA"
                     /locus_tag="ACHJ12_001813"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4989610.1"
                     /translation="MFPKIMNDENFFKKAAAHGDEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43086..43406)
                     /gene="psiF"
                     /locus_tag="ACHJ12_001814"
     CDS             complement(43086..43406)
                     /gene="psiF"
                     /locus_tag="ACHJ12_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4989611.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43525..44940)
                     /gene="phoA"
                     /locus_tag="ACHJ12_001815"
     CDS             complement(43525..44940)
                     /gene="phoA"
                     /locus_tag="ACHJ12_001815"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO4989612.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45041..45301)
                     /gene="iraP"
                     /locus_tag="ACHJ12_001816"
     CDS             complement(45041..45301)
                     /gene="iraP"
                     /locus_tag="ACHJ12_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4989613.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        11196 a        12160 c        11428 g        10567 t
ORIGIN      
        1 gaatgcaaaa aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa
       61 cactctgttt acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg
      121 gtaatgaacg attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc
      181 gtattctcgt tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc
      241 atggctctgc aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg
      301 actcaggccg tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca
      361 ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac
      421 tccatctggg gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc
      481 gttatggcgc tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt
      541 atcggcgtga gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact
      601 cacaaacttg ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt
      661 gcgttgacca tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga
      721 atggtgaaag gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt
      781 ggcattatgt gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta
      841 gctgatgcgg ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc
      901 gcctttggct cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa
      961 gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa
     1021 acgcagttct ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg
     1081 gaagcccttc tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg
     1141 atgggtgtct actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc
     1201 tggattgacg gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca
     1261 gtgtggctgg cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt
     1321 gaaatcccgg cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa
     1381 ggcatcaaag aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc
     1441 aaagtgacga atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga
     1501 attgcctgct gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag
     1561 gccgaatcag gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat
     1621 cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga
     1681 cagcctgcaa atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg
     1741 cctgcacttt cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct
     1801 caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct
     1861 ccggtacatc cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat
     1921 ccagcaactg attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt
     1981 cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc
     2041 tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag
     2101 atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat
     2161 aaccgacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac
     2221 aattaaggga tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa
     2281 attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag
     2341 ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt
     2401 gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc
     2461 accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc
     2521 ctcgcgtcca cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc
     2581 atcgaagagt tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc
     2641 cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca
     2701 cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac
     2761 gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg
     2821 attaatccac tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa
     2881 gaaattatgc agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact
     2941 tcagcagaag atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc
     3001 tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt
     3061 tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt
     3121 gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc
     3181 tggtagtgaa gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa
     3241 agtcaccact gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt
     3301 gaagctgctg gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt
     3361 actgcctggt ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac
     3421 cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac
     3481 cgtgctggtg ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa
     3541 agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa
     3601 attgctgacc agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct
     3661 gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat
     3721 ttataattat tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt
     3781 tacaaccaca aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata
     3841 gagcgcctgc agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg
     3901 gtcgagatcg ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc
     3961 ttcgactttc agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc
     4021 gttcgggccg aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc
     4081 gatttcgcgg atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc
     4141 gaatttctct tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga
     4201 gataacaccg caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc
     4261 caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga
     4321 gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc
     4381 gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat
     4441 catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac
     4501 gaccggttcg aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca
     4561 cagataatgc gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa
     4621 acagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa
     4681 accaccggaa atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc
     4741 ttcgtagcgg cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac
     4801 ttccggattg gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac
     4861 gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca
     4921 gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg
     4981 catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt
     5041 cagggcgtcg cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc
     5101 ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc
     5161 ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa
     5221 caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta
     5281 tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta
     5341 acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt
     5401 ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa
     5461 tgaggctccc gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct
     5521 ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct
     5581 ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc
     5641 tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg
     5701 cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca
     5761 ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt
     5821 aagcgtctgc gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac
     5881 acgctggacg cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat
     5941 gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag
     6001 tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt
     6061 ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa
     6121 ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg
     6181 gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca
     6241 ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct
     6301 ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac
     6361 atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa
     6421 cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg
     6481 gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc
     6541 tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt
     6601 aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     6661 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     6721 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     6781 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     6841 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     6901 accatccgtc agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac
     6961 gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct
     7021 gccgagaaag aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc
     7081 gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     7141 gtcctcaacg acaatgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa taaccatctg
     7201 gcacagctgc tttccggtaa gctttactct tcgctgcgcg aaggcgggaa aaaagttttc
     7261 tctggcgttc cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg
     7321 gtagtgcctg gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt
     7381 cacgatgtgc tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag
     7441 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc
     7501 actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc
     7561 ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac
     7621 aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca
     7681 cgtaaattcc cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt
     7741 gccgccggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg
     7801 caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc
     7861 gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac
     7921 ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa
     7981 tgtcgccaga tgctctatac cggctatcac tataacgatg gcccgtcagc ggtgcgctac
     8041 ccgcgtggca acgcggtcgg cgtggaactg acgccgctgg aaaaactacc aattggcaaa
     8101 ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca
     8161 gaagcggcga aagtcgccga atcgctgaac gccacgctgg tcgatatgcg ttttgtgaaa
     8221 ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta
     8281 gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat
     8341 cgtaaaccag tacccgtgct gaacattggc ctgcctgact tctttattcc gcaaggaact
     8401 caggaagaaa tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag
     8461 gcctggctgg cataatccct actccactcc tgctatgctt aacaaattat tcatagactc
     8521 taaataattc gagttgcagg aaggcggcaa acgagtgaag ccccaggagc ttacataagt
     8581 aagtgactgg ggtgaacgaa tgcagccgca gcacatgcaa cttgaagtat gacgagtatg
     8641 gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac
     8701 tttgcctcgg ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc
     8761 cggaagaaag cagccgtccc ataattaaac gcgcgctgga aggcggcata aatttctttg
     8821 ataccgccaa tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg
     8881 atttcgcccg tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt
     8941 taccggaagg attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc
     9001 tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg
     9061 aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg
     9121 cgtcatccat gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct
     9181 gggcgcagtt tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg
     9241 agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc
     9301 gggggcgact gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg
     9361 ggagaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg
     9421 gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttgagta
     9481 aaccgggcat tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctat
     9541 tgaacgcggt ggatatcact ttgaagccgg aacagattgc cgaactggaa acgccgtata
     9601 aaccgcatcc ggtagtagga tttaaataag agtccaggcc tgatgagacg caacaagcgt
     9661 cacatcaggc atcggtgcac aactacgaca gaatgcccag cggccagtga tgaccaataa
     9721 agtacaggat gccagcggag atcaccccgg cgacaatatc atctatcatg atccccatgc
     9781 cgccatgcac attgcgatca aaccagcgga tcggccacgg tttccacata tccagaatac
     9841 ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg
     9901 tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat
     9961 cttttgcggt ctgatgacaa agatagacgc cgatacagat ccccagcatc accaccagcg
    10021 agtagagctg ccagggcaaa aaggtcatca gataccagaa cggaatcgct gccagcgagc
    10081 ccatcgtccc aggaacgatc gggcttaatc cacttccgaa tccgacagca agcagatgcc
    10141 acggattact catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca
    10201 tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
    10261 tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc
    10321 agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag
    10381 agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc
    10441 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
    10501 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
    10561 tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca
    10621 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta
    10681 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
    10741 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
    10801 atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc
    10861 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
    10921 atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg
    10981 atatcaggga ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt
    11041 ttctcgggga tattgagaag tgcgcaatcg tcgccgatac ccagttcgac atcaagacga
    11101 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
    11161 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacggtctt ccgtgaatct
    11221 accggcctgg atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
    11281 cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt
    11341 taatggccac tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca
    11401 gtactgcttt ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca
    11461 gtccgtcgag gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga
    11521 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
    11581 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
    11641 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
    11701 atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
    11761 cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg
    11821 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg
    11881 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
    11941 gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg
    12001 ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg
    12061 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
    12121 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
    12181 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
    12241 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
    12301 ggcgtcggct aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa
    12361 tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc
    12421 agcgagcgtt ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat
    12481 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
    12541 ttccggccac tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg
    12601 cacaatgcga tgttccggca tcacgcgatt ttggctatca atcacaatac gtactggctg
    12661 gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac
    12721 tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg
    12781 tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc
    12841 gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa
    12901 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag
    12961 cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg
    13021 cgggttagga tcttgcatcg aggcaaccac gcgcgctacg ccagcggcga tgagtgcgtc
    13081 acagcacggt ggcgtacgac catgatggct acagggttcg agtgtgacat aggcggtcgc
    13141 acctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc
    13201 acgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
    13261 cgggttggga tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta
    13321 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
    13381 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
    13441 caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc
    13501 gcgcagctgc gatttaatat gattgattgc catttcgacg tcatcggaac tcaccggacg
    13561 tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac
    13621 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
    13681 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
    13741 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
    13801 ccaggtggtt aacagaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
    13861 ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg
    13921 acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg
    13981 ttcagcctta gcgcctgtgt gcagcagagc gaagtgcgac agatgaaaca cagcgtcagc
    14041 acgctgaacc aggagatgac gcaactcaat aaagaaacgg tcaaaatcac tcagcaaaac
    14101 aggctgaatg caaaatccag cagtggggtt taccttctgc caggagcgaa aacaccggca
    14161 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgatgca
    14221 gatggcacca ccctgacgct acgtattcag ggcgagtcga atgatccctt gcccgcattc
    14281 agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
    14341 gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt
    14401 ccgttacagt taaagggtat ttctgtagaa cagttagact tcgtacgtat ccacgatatt
    14461 caacctgtta tgcagtaaac gtatttcggg gcgattttgt gcgtctcgca acatctttcc
    14521 ccgtcatttt gttactccgc ttacatcacc ctgattgata gtaaaagttt gcaacaaggg
    14581 tgaaagtcag tacaatccct gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa
    14641 atgatagaac tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct
    14701 ctgtatacga aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc
    14761 aaacagtggc atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc
    14821 ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg
    14881 gcaccagagc gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa
    14941 cgatacctac cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta
    15001 tgcggatgcg ccggtattct tcggcggtaa ctccgatgca aaaggtatct ggaaccacgg
    15061 ttctccgctg tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga
    15121 ccttagcttc ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg
    15181 tcgtaataaa gatggtcgcc agagcacctg gtacatgggt ctgggtaccg atatcgacac
    15241 tggcctgccg atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg
    15301 cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat
    15361 taccgatctg tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc
    15421 cgatttaggg gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc
    15481 tatcgcttcc agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc
    15541 tcgttactgg cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg
    15601 caacttcaac gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg
    15661 attatgaaaa tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt
    15721 tcatgccgga tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata
    15781 ttgctgaaat aacgtagata ctgataagcg tagcgcatca ggcaatttta cgttttaatc
    15841 acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga
    15901 catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat
    15961 atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta ctgccatctt
    16021 ccgggttatt ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc aggttggaat
    16081 aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc
    16141 gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaaa agttacctgg
    16201 atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca
    16261 tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattacgg
    16321 cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg
    16381 cttcataccc agtttcagag ccaacgcaga tgctacgtag atggaagatg cagtgccgat
    16441 ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagagata
    16501 cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg
    16561 ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc
    16621 acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa
    16681 cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat
    16741 gataacgtcg tgcgccagcg caataacaac ccctgccgcc agtcgccact caaagcgaaa
    16801 acctacgtac acaaggatag acagcagcgc cgccatcaac gccatcgcac cggtttgcgc
    16861 aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt
    16921 ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccgc cggtttcgcc
    16981 ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg
    17041 ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc
    17101 gagcgtaatt tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac
    17161 gcccataata acgatagcgg cgattaacag cagaccagag atgccgaaag cccagtagtc
    17221 ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg
    17281 tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt
    17341 ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg
    17401 tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa
    17461 tcagcgtggt gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa
    17521 ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta
    17581 cgttcgcatc gaccgccacc gcaagggtta agacgatacc cgcgatacct ggcatactca
    17641 gcgttgcgcc tggcaacagc gacataatgc cgacgattaa gatcaagttg gcaatcagag
    17701 cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca
    17761 gcagaccggc aaggcaagcc tccagcccct gttcaatgtt ctgcataccc agggttgggc
    17821 caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca
    17881 gtgacagctg acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca
    17941 gacgagactg gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac
    18001 catttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc
    18061 cgatgttgtc cttagtgaag ttagacatga tgttaccacc agcgctatcg agcgagatgt
    18121 taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg
    18181 tcagaattac gcgtttgtac agcacaactg gctgaccttc gcgggtctgt ttcacttcag
    18241 agtcgcccgg tacgcgaccg gatgccgcag cggcctggtc aacgttggtg tttaccagac
    18301 ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg tcctgaatac
    18361 ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc
    18421 caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca tattcacgcg
    18481 cttcactcag acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctactaa
    18541 tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat
    18601 cgcggaaagt aatgctcagg ccgtagttgt tttctttacg aacggtggta tacgggatgc
    18661 ccttttcacg cagatcgctg cgcaggctat cgatattttg ttcctggagt ttgccaagcg
    18721 cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct
    18781 tcatcggctc agcgtgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg
    18841 ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc agctgggtgt
    18901 cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta
    18961 ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc
    19021 cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca
    19081 gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta
    19141 acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca
    19201 ggacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag
    19261 caatgtagcc gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa
    19321 cttcatcacc tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct
    19381 gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag
    19441 acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc
    19501 tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac ttctcgcccc
    19561 tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt
    19621 aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct
    19681 aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg
    19741 tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc attgcggatt
    19801 ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg
    19861 cagtcaaaca tatcgatacc acgacgtacg ccttcaacca ggtcttctgg tttaccaacg
    19921 cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc cagaatgcgg
    19981 tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaaaccg
    20041 atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacgct gccctgaatg
    20101 ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc
    20161 caacgcagag acatctccat ggagcgtttt gcgtaatccc agtcagcagg atacggcgta
    20221 cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc cattgatttt
    20281 tcaggatcga ggaaaatcgg atcgccgttg atcgggttac ggaagtgcac gccctgttcg
    20341 gtgattttac gaatatcgcc aaggctgaag acctggaaac cgccggagtc ggtgaggatc
    20401 ggccccttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc
    20461 gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact
    20521 tcttccggcg tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt
    20581 tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc
    20641 agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc
    20701 gccgcggaat tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat
    20761 cgcatcaccg taactaaaaa agcgatattt ctcttctacc gctgctttat aggcgttcat
    20821 ggtgtgttga taaccggcaa aggccgaaac cagcataatc agcgtcgact ctggcaagtg
    20881 gaagttcgtc accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg
    20941 tgtatcgtcg aagaacggtt caatgagatc gtttttcgct gcctgagccg cgctttccag
    21001 cgaacgtact gaagtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag
    21061 taccgcgtct accacatcct gcggtacttc agcgtattcc gagtgcatga tgtgatcttc
    21121 aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc
    21181 catctccacg cctttggcgc gcaatttttc cagcaaaggc tcgtcaaaat gcagaccagc
    21241 ggtcggggct gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc
    21301 agcgtcttcg tccggacggt cgatatacgg cggcagcggc atatggccga tactgttgag
    21361 gatatccagt accgaacgat catcattgaa ttcgacttca aacagtgcgc cgtggcgcgc
    21421 ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg
    21481 cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag
    21541 cacttcaatc ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt
    21601 attattaaaa accagaagat cgccggggtt gagcttatca agtaaatcgg tgaaagtacc
    21661 gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg
    21721 catgggatag tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat
    21781 gacactgact cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca
    21841 acctttaccc ccttttggat agaatataat cgatgaattt tttagctcac ctgcatttag
    21901 cccatctcgc ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc
    21961 ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg
    22021 tattaaccga caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaactc
    22081 gccgcgttgc gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt
    22141 cgcagctgtc gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga
    22201 tgacgatttt gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag
    22261 agcagtggct ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg
    22321 caagccgccg cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt
    22381 atgacgccct cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac
    22441 acaaggcgtt ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag
    22501 aatcccgtca gtcagttgcc catgcccttc tttgcattgc cattgcacgg cattgagcaa
    22561 cggtgacgcg ggtagcacga cttcacaagc ttcgccacgg ttgattgcca ccagtacacg
    22621 ctgctgattc agcacgcgga caaataccac cacgttatct tccgcataca gcacctgaca
    22681 gccgccatga cgtagcgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc
    22741 gaataacgcc gtatcctgct tttccacctg ccaagggaac ggtttacggc aaaacggatc
    22801 gtttttgcca tccagtccta cttcatcacc gtaataaatg cacggtacac caggccaggt
    22861 gaacagccag accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa
    22921 tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag
    22981 ccctgcgcgg taattatcca tccaggccat acaggtttgg gcatcaattt gctgcggatc
    23041 gtaagagata tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt
    23101 catggcggca tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc
    23161 gacaatatac gcttccggct gggtttcttt cgccgcttcg gtgatcccag caacgtgctg
    23221 catattattg cgcgccccac ccgcctcccc cagcatatgc accacatcca gccgccagcc
    23281 gtccatactc cacggcgctt tcagccaatg gcggacaata ctgtcttccc cgcgataaat
    23341 ttcattcacc agactttccg actgataatc cagcttcggc aagctggcat agccaagcca
    23401 gtcgagcgcc gtgccatcat cactaaacga gtaccagtcg cgccagggcg attcagggtt
    23461 gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac caggcatggg aatcgccact
    23521 gtggttaaac acgccatcca gcaccagccg cattcccagc tgctgcgtat tgtggcgtaa
    23581 gcgcagcaac gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc
    23641 gtatttatgt acgctgggag ctttaaacac cggattgaga tacagcgccg tcacgccaag
    23701 ctttttcaga tacggcagtt tttcgcttat cccgtccaga tcgccgccat agaacgttga
    23761 tccgcccgcc tgcgccgtga ccggttcatc ccagtcacgc aagatgatct cttgtccggc
    23821 tgcatgatgg taatagacat gatcctgttc agcttcacga ggaagactac gcgcaaaacg
    23881 atcagggaag atctgataaa aaatctgatc cgcagcccat tgtgggccga tatccggtac
    23941 atcgacggca aactgctcca gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa
    24001 ccaacgctgg cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg gttgtccgct
    24061 ggagagatca atcgccgcac gccatgcggt gacgccaggc tgctgctgac tgcgctgctt
    24121 atgcatcgat actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg
    24181 tgggtcttcg cccgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa
    24241 tgggggcacc ggcaggtgcc atgcatttaa catcataatt cccctgtgat gaaaatctga
    24301 tcaccttgcc atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag
    24361 gacgggggcg gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca
    24421 gaacgcaagt attattggtt ttcagccagc tgacgatcgt ggcggcgttt aaacatccag
    24481 ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca gcgagatacg cgtatccggg
    24541 tgataaccaa tcaacccgat aataaagagc aggaaaatca gcccgccgat ggtcgttgct
    24601 accccacccg gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acggcgacgg
    24661 aaggcaattt gcgacagcag gatcataatc cacacccaca ccgtggcgaa ggttgccagc
    24721 gaagcgatca ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc
    24781 agcgcggtag tcatcaccag caccgtaacc cacggaatac cgcgacgcga cgttttgctg
    24841 aaaattttcg gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacgccaaat
    24901 acgtcactgt taattgccga cagcgaagca gtcagcacaa caaagttaag aatgctggcg
    24961 gcaaaggtaa tgcccatatg ctggaacgtc agcacgaacg ggctaccggc agtgccaacc
    25021 tgattccacg ggtagataga cataatgacg aacagcgtac cgacgtagaa caccagaata
    25081 cgcatcggca cggagttaat cgcacgcggt atcgatttct caggatcttt cgcttcaccg
    25141 gcggtaatcc cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatc
    25201 accatcccaa gccagccgtt actgaagaag ccgccgttgc tccacaggtt atgaataccg
    25261 gtcggttgcc cgccgttgcc aatcccccag atgatgatgc cgaaaccggc gacaatcatg
    25321 atgatgatgg tggcgacttt aaagaacgag aaccagaatt ccagctcacc gaagaccttc
    25381 acgctcatca ggtttacggc gcaaatgatc agcaccacgc tcagtaccca aatccagtgc
    25441 ggcaccgtcg ggaaccagac acccatatag ataccaaaag cggtcacatc ggcgatggcg
    25501 acaataagga tttcaaagca ataggtccag ccggtaatgt aacctgccag cgggccgagg
    25561 ttttcctgcg cataacgcga gaaagagctg gctgccgggt tatgtaccga catttccccc
    25621 agcgcacgca taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctc
    25681 ggaccggcca ttttgatggc gtctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa
    25741 cccagtgcca taaagcgtat gtggcgggta cttagcccac gctttagctt gttcttactt
    25801 tccatcaatt cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt
    25861 taaaacaaat gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga
    25921 tcccagataa tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt
    25981 tcggccagcg gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc agatgctttg
    26041 atcccgtcga gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac
    26101 gaattatgcc accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa
    26161 atagcggtca gcaccggtac ggagatctgg atcagctggc ttaagccgag gttagaaacc
    26221 accatcgaga agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg
    26281 gcaaagaatt ctgcacaagc acaggtcagg ccaactgccg ttaccaggca ggcgatgaag
    26341 attaacgccg ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc
    26401 agaatagcag cgccgtttgc agactgatcg accagcgacg cgctgtctga acccagacgg
    26461 aacagcgcca ggtacagcag agtcagacca acacccgcca tcaggccagc ccagacggta
    26521 taacgggtca gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg
    26581 ataccaaaca ccattgcgcc cagcgtatcc atggtcagat agccgttaac gaagccgtta
    26641 gaaaacgcag cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagaca
    26701 atcgcggcaa cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc
    26761 acggtatcca gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc
    26821 aggctgtaga taaacagcgg cagcgcggaa tcaccagtca gcggcgcaat ccccacttca
    26881 aaggaaacgg tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc
    26941 gttgccagca gtacgccagc gactttaccg attggcgtgc tgaggctgtc aacaccgccg
    27001 ccaacttttg ccagcgccac taccgttagt accggcagac caacggcagt aatgaggaag
    27061 ccgaatgccg cagtccagac gtgttcgcct gcctgcaagc cgaccattgg agggaaaata
    27121 atgttacctg cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc
    27181 gatcttaatt gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat
    27241 ttttgtgcca atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata
    27301 ctcccaacca ggcgcggcga agaatagttt ttcgatttac cgcgttaatg cagtcagtat
    27361 gacagcacct gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga gggatctaaa
    27421 aaccagaggt atatagcgat aagaaatcac caaccagtat aaaacactat ttccgtgaaa
    27481 aagacatggc taatcatgcg aacaaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt
    27541 attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa aagatgacaa
    27601 aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg
    27661 ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc accgcatgtt
    27721 tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc
    27781 gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt ccgttatctt
    27841 caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata tgcgtgcctt
    27901 ctggagtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt agctgatctt
    27961 cgttgccaga cactttgagg ccgttatcta tctcaaaggt aaatgtctgt tttttctgac
    28021 tcagagtctg agcctcacgc tcaacaacgc gcagcatcat cggcacatca accttttcat
    28081 tgagcaaatg cgtcggtgcg gcttctattt tcgacagcgt cagcaactgc ttcaccagcc
    28141 cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt
    28201 ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat ggcgtacgta
    28261 actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga tgcatttgcg
    28321 tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg cgaatttcca
    28381 gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc gttttcagat
    28441 attgcgtaaa ctccgggtaa cgcagcaggt taaggatgtt ctgcccgtta tcttccggcc
    28501 agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg ccctcttcag
    28561 tggtcagcac cactgcgtcg ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat
    28621 tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag ccgtatagta
    28681 gcggttccca gctaccacgc cccggtggcg gggtcatact acgatccacc cacagccacc
    28741 atgaaaggcg caataaattc cagaaatgcc agataagcag tcccgttacc gatgccagca
    28801 aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggaag cagcaaagta
    28861 gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat actccagtta
    28921 agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac
    28981 gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgcctggc tccagtgctt
    29041 tacgtaggcg acgaatgtgg acatcgaccg tacgatcttc cacatacacg ttagttcccc
    29101 agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt
    29161 gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc atcactcggt
    29221 gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc gccattggcg
    29281 aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg
    29341 tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg
    29401 cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg tgtttgatga
    29461 actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc ggccagggtt
    29521 cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga aagccatttt
    29581 gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg accagaatac
    29641 gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg cgacgtcatt
    29701 atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc aggttattga
    29761 tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg cgcccatgct
    29821 ttttcgccag ggaatcgtta tgcgcatcct tcacacctca gactggcatc tcggccagaa
    29881 cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc tgctggaaac
    29941 agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg ataccggctc
    30001 gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac agcaaactgg
    30061 ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga atgaatcgcg
    30121 cgatatcatg gcgttcctca atacgaccgt ggtcgccagc gccggacatg cgccgcaaat
    30181 cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc
    30241 gcgtgacatt attaccagcc aggcggggct taacggtatt gaaaaacagc agcatttact
    30301 ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac tgcgcggcga
    30361 tcagcctctg cccatcatcg ccacgggaca cttaacgacc gtgggtgcca gtaaaagtga
    30421 cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccagcacaaa actttccacc
    30481 agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg gcatggaaca
    30541 tgttcgctat tgcggctctc ccattccact gagttttgat gaatgcggaa agagtaaata
    30601 tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc tgaacgtacc
    30661 ggtaacgcaa cccatggcgg tgctgaaagg cgatctggcg tcgattaccg cacagctgga
    30721 acagtggcgc gatgtatcgc aggagccacc tgtctggctg gatatcgaaa tcactactga
    30781 tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat tgcctgtcga
    30841 agtattgctg gtacgtcgga gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg
    30901 tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga
    30961 actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt tgcataccct
    31021 cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct gaactcatta
    31081 aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg gctgtttgct
    31141 attaccggcc caacaggtgc ggggaaaacc accctgctgg acgccatttg tctggcgctg
    31201 tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct catgacccgc
    31261 gataccgccg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca
    31321 ttctggagcc agaatcgggc gcgtaaccaa cccgacggta atttgcaggt gccacgcgta
    31381 gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa
    31441 ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat gctgctttcg
    31501 caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga attgctcgag
    31561 gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga gcagcacaaa
    31621 tcggcccgca cagagctgga gaagctgcaa gcgcaggcca gcggcgtcgc gttgctcacg
    31681 ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga agaaaaacag
    31741 ttacttaccg cgcagcagca agaacaacaa tcactaaact ggttaacgcg tctggacgaa
    31801 ttgcagcaag aagccagccg ccgtcagcag gccttgcaac aggcgttagc cgaagaagaa
    31861 aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa tcttcgtcca
    31921 cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca gcagattgaa
    31981 gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg ccaccacgcg
    32041 gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg gttacaggaa
    32101 cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa
    32161 caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca tgctgagcaa
    32221 aaacttaatg cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt tgctaccgcc
    32281 ctggcgcaac atgctgagca acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag
    32341 attgttcccc aacaaaaacg tctggcgcag ttaatggtca ctatccagaa tgtcactctg
    32401 gaacagacgc aacgtaatgc cgcacttaac gaaatgcgcc agcgttataa agaaaagacg
    32461 cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcattaa aacgctggaa
    32521 gcccagcgcg cgcagttaca ggcgggtcag ccttgcccac tttgtggttc caccagccac
    32581 ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg attactggcg
    32641 ctggaaaacg aagttaaaaa gctcggtgaa gaaggtgcga cgctacgtgg gcaactggac
    32701 gccttaacaa agcagcttca gcgtgatgaa aacgaagcgc aaagcctccg acaagatgag
    32761 caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac cttgcagcca
    32821 caggacgata ttcaaccgtg gctagatgca caagatgagc acgaacgcca gctgcggtta
    32881 ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca aattatccag
    32941 tatcaacagc aaattgaaca acgccagcag cagctcttaa cggcattggc gggttatgca
    33001 ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca gcaagaagcg
    33061 cagagctggc agcaacgcca gaacgaatta accgcgctgc aaaaccgtat tcagcagctg
    33121 acgccgattc tggaaacgtt gccgcaaagt gatgagctcc cgcacagcga agaaactgtg
    33181 gcgctggaaa actggcggca ggttcatgaa caatgtctcg cattacacag ccagcagcag
    33241 acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca ggcgcagttt
    33301 gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc ggcgctaatg
    33361 gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa ccagcgccgt
    33421 caggcgcaaa cgctggtcac tcagacagca gaaacgctgg cacagcatca acaacaccga
    33481 cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt agcgcaaact
    33541 caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca gctgaagcag
    33601 gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca aatgacgcag
    33661 caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg cgataaattc
    33721 cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa tcagcaactt
    33781 acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct ggaagtcgag
    33841 gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc cggcggcgaa
    33901 agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag ccataaaaca
    33961 cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga aacgctggat
    34021 accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg tgtgattagc
    34081 cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa gatcaacggc
    34141 ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag caggataatg
    34201 aatacagagg ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa gctatcactt
    34261 tattggctac ggtgattggt agccgttctg gtggttgtga tggtggtatg aaaaaagtca
    34321 ttttatcttt ggctctgggc acgtttggtt tggggatggc cgaatttggc attatgggcg
    34381 tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg catatgatct
    34441 cgtattatgc actgggggtg gtggtcggtg cgccaatcat cgcactcttt tccagccgct
    34501 actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt
    34561 tcacgctctc ttcgtcttac ctgatgctcg ccattggtcg gctggtatcc ggctttccgc
    34621 atggcgcatt ttttggcgtc ggagcgatcg tgttatcaaa aattatcaaa cccggaaaag
    34681 tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg ctgggcattc
    34741 cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta ttgatcgctg
    34801 tttttaatat tgcggtgatg gcatcggtct atttttgggt gccggatatt cgcgacgagg
    34861 cgaaaggaaa gctgcgcgaa caatttcact ttttgcgcag cccggccccg tggttaattt
    34921 tcgccgccac catgtttggc aacgcaggtg tgtttgcctg gttcagctac gtaaagccat
    34981 acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg atgttagttg
    35041 ggctagggat ggtgctggga aatatgctaa gtggcaggat ttcaggacgt tattcaccac
    35101 tgcgcattgc agcagtgact gactttataa ttgtactggc actgctgatg ctctttttct
    35161 gcggcggcat gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg ggattatttg
    35221 ccctttcagc accgctacaa atattgttac tacaaaacgc caaaggcgga gagttattag
    35281 gtgccgcagg tgggcaaata gcgtttaacc tcggtagcgc cgtcggcgca tattgcggtg
    35341 gtatgatgct gacgctgggg ctggcatata attacgtggc gctgcctgcc gccctgcttt
    35401 cgtttgctgc gatgtcgtcg ttgctgctgt atggtcgcta taagcgccag caagcggcgg
    35461 atactccggt gctggcgaaa ccactggggt aggttatagt ctcggtgctc attacttatt
    35521 gccggatgcg gcgtgaacgc cttatccgcc ctacgcggtt ctggcacatt ttgcaggcct
    35581 gataagacgc ggcaagcgtc gcatcaggca tgatgcgcca attgcctacg ttttttactc
    35641 ttgtggccat aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct tcgccttacg
    35701 caccggcgtt tcacattcgc cgccgaagac aaattgttta atcaactgcc caaccgtttg
    35761 atataaacgg tctacattgc tcatcccgcc ccccaggaca atcacatccg gatcgagaat
    35821 attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc
    35881 cgctaccgga tcgctttctt caaccaggct gataatttca ctgcctttca gcgcatgtcc
    35941 gctcaaacga cgataatctg tggcaaatcc cgtgcccgaa ataaaggttt caatacaacc
    36001 ttgtttaccg caataacaag ggacttcctc gcgataacgc agttcgtctt cgtccatcca
    36061 cggtagcgga ttgtgtcccc actcacctgc cgtgccattg ccgccgatat gcgcccgccc
    36121 attgaatgcc acgcccgcgc cgcatcccgt gccgataatc acggcaaata ctgtctgcgc
    36181 tcccgctgcc gcgccatcta ctgcttctga aaccgccaga cagttggcgt catttgccag
    36241 ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac cgttgagcca
    36301 ggttgaattg gcattcttaa ccacaccggt gtaaggcgaa attgagccag gaatgcccat
    36361 acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg tggcgatcgt
    36421 ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc
    36481 ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg
    36541 cacggtactc tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc
    36601 aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa tttaacgaca aggccgtgga
    36661 aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct
    36721 gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca
    36781 ggacatggcg aagatgggct gggttcctcc gatgggatcg cacagcgatg cgttaacgca
    36841 cgttgccaat ggtcaaattg ttatctgcgc acgcaaagaa gaaaaaatcc tcccgtctcc
    36901 ggtgattaaa caggcgctgg aagcgaaaat cgccaagctg gaagcggaac aggcgcgtaa
    36961 gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgctgccgcg
    37021 agctttcagc cgttttagcc agacaatgat gtggatcgac acggttaatg gtttgattat
    37081 ggtggactgc gccagcgcca aaaaagcgga agatacgctg gcattactgc gtaaaagcct
    37141 ggggtcgtta cctgttgtac cattgagcat ggaaaacccg attgaactga cgctaaccga
    37201 atgggttcgc tccggtagcg cagcacaggg cttccagttg cttgatgaag ccgagctgaa
    37261 atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga
    37321 gatcaccaat cacatcgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca
    37381 gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag cgtctgaagt tctgcgacga
    37441 gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gcccagcgtt ttgatgccga
    37501 tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg
    37561 tggcgaagca caacgttaat tgctgatttt cctttaatgc cggatgcgac gcctgccgcg
    37621 tcttatccgg cgtacgaagc cacaccaggc atataattat tcgctacggc gggcaataat
    37681 ttttagcgca gcaatattat gcgttttatg ctgtaacttg ctccatggac gttgtgtcat
    37741 tgtttttcct caagccagtg acgcgcttca ctgcgaattt tccgcattgc cccggttttc
    37801 actaaatccc gtaacagcga aagatcgggt tgttcgacaa ataagagtaa tgatggatcc
    37861 tcttttaacc acgtcgtttt cacatcggca gcgagctgtg gattattcat agctagcgac
    37921 cgatgccgag actcggtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    37981 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38041 gatttatctt ttatcgtagc cagttccgca acaatatctg gagatcttca ctgatattta
    38101 tatcaacctt gatgaattat gaatatatcg gaaattgaaa aaatcgcagc aaaggaaata
    38161 ttattatggg ttaaagagag gatatttcag aaaaatgccc tcccgttcaa ggggaaggcg
    38221 aggaattaca gatagcggca cagataagag gtgggttcgg caacttgcag atgaaactca
    38281 ctgtgaccgg gaacattaaa caccgaaccg gcttcataca cctgccagtc ggtcgcgtca
    38341 ggcagtaaca cattcagcgc gccactgatt accgtcatct cttccggctc agcggtgctg
    38401 aaggtgtatt cgccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa
    38461 aagccgattg atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc
    38521 tgtctggtag ttgaacatct acttcactat aggggccaga ggcgctggct gtcacgcaaa
    38581 attacaccag taattccgca gccagccgtg caatcaatac gttagacagc aagacaggaa
    38641 catcgagctg tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa caatccagca
    38701 tgatgacatc agcccctttt gccagtaatt ctttcccggc atcaatgatt ttttgttctg
    38761 aatcatgaat ggggttaccc aatgaaaata ccggcgattt ctgcaaaatt tgccattttt
    38821 gcgcctgaac ggtcagcatc tcctcaaccg gaacgataac ccccacctga tgatcttcaa
    38881 caatagagga aaccagtgga ggcaatattc gcgacggctc aagaaagatc gtattacgcg
    38941 cagtcatact actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat
    39001 cgagcacttc aaccacacct tgcaggtctc gctccacttt acgacgcgaa acatgggcca
    39061 gctggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg
    39121 ccatcacttc ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt
    39181 caatgtattc cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg atggtcagga
    39241 tcgccaacga cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg
    39301 caagtgtgaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag
    39361 cagacgtgca cggcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt
    39421 taatattcta attattagaa aaacatgaat tatgaaaaat gtgacgcaga tcccttgtag
    39481 tggcactcag aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata
    39541 gcgttttttc gcatccatcg cttcctgagc cgtcggatgt tcactgatca acgagtctgg
    39601 tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttttc
    39661 aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta
    39721 tttcacggga tgaacgttaa gtataggcgc tctaaaatca acaattgatc gtctgtgcca
    39781 gggcgctacg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat
    39841 gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca
    39901 gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa
    39961 ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac
    40021 gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat ggcgcagtta
    40081 ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga
    40141 tctccgcgac cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac
    40201 ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga
    40261 tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc
    40321 cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa
    40381 aattccactt aaagtgaaaa tctcaataca tcccttacta taccaataaa tattcaagaa
    40441 tgaagtgtaa ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt
    40501 ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt
    40561 ctggatccca ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat
    40621 ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc gctgcgcctt tctcgatggc
    40681 ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac
    40741 aatttcgtta tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga
    40801 tggcggcacg cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc
    40861 cgcgcgatac aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt
    40921 cataaagtca tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata
    40981 gttctgttta tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg
    41041 caatggaaaa gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg
    41101 gtctgattgc cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccgg
    41161 ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag
    41221 tggcgcaaat cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc
    41281 ttagcgaaat cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg
    41341 tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc
    41401 cgaacactcc cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa
    41461 ccccagaaga taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa
    41521 ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtat
    41581 ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg
    41641 cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag
    41701 aacatccggg ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg
    41761 tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta
    41821 tggaaaaatc agaaaaactc agcaaatcct gatgactttc gccggacgtc aggccgccac
    41881 ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa
    41941 ccactcacga tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa
    42001 agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat
    42061 ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc
    42121 aaaccgacca atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac
    42181 aatcgcttca tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat
    42241 atcgataatc agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg
    42301 taacatagtt tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gggtactcat
    42361 gacctgcaac ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc
    42421 aaacagcaga ggataaacga caataatggg aagggagagc caccattcca gcggcgcact
    42481 attgaacgac acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa
    42541 caccagaccc gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat
    42601 catcaacata gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc
    42661 taatactgca tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct
    42721 cgctatctgc caggctaaat gcggccagac gaacgcccag ccgaccaaca ccagccacca
    42781 ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag
    42841 gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc
    42901 gttttgagga gttaaaggag gttcatcccc atgcgccgcc gcttttttga aaaagttttc
    42961 atcattcatt atttttggga acattcagaa acaattttcc caaattatag agacggggct
    43021 aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta gctcacccgt tagcactatc
    43081 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
    43141 agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
    43201 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
    43261 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
    43321 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
    43381 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
    43441 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
    43501 gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca tggtgtagaa
    43561 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
    43621 acgcaactga ctgccggtat gttcttgtga atcctcttcg gagttcccgt agctcatcac
    43681 catcactgcg ccatctttgg tatttagcgc ctgggtgagg cccggagctt tggtatccgg
    43741 cgcgacaatc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttgccatc
    43801 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
    43861 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
    43921 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgcgccag
    43981 ggttggtaca ctgtcattac gttgcggatt aggcgtacag gtgactgcgg gcttatcgat
    44041 attgccgtgg tacgttgctt tcggtcctag ccagcgcact ggcatattgc cgtcagcaaa
    44101 cagtccaagc agaggttttt gctgattcgc ttccgtcacc gcatttagcg aggcggcatc
    44161 gctgaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
    44221 accggcggtt gccgtttcag caaaggtttt tgcgccaccg ccaagcgtaa cgtcggcacg
    44281 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
    44341 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
    44401 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
    44461 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
    44521 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtgac
    44581 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
    44641 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
    44701 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
    44761 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
    44821 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
    44881 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
    44941 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
    45001 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcttca
    45061 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
    45121 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
    45181 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
    45241 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
    45301 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac a
//
LOCUS       DAZFFI010000027        42721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFFI010000027 DAZFFI010000000
VERSION     DAZFFI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.027"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..837)
                     /locus_tag="ACHJ12_001817"
     CDS             complement(<1..837)
                     /locus_tag="ACHJ12_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4989614.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSELVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQRTASLSSPDTPRPL
                     SAS"
     gene            complement(1267..3681)
                     /gene="ybbP"
                     /locus_tag="ACHJ12_001818"
     CDS             complement(1267..3681)
                     /gene="ybbP"
                     /locus_tag="ACHJ12_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4989615.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLKVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(3678..4364)
                     /gene="ybbA"
                     /locus_tag="ACHJ12_001819"
     CDS             complement(3678..4364)
                     /gene="ybbA"
                     /locus_tag="ACHJ12_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4989616.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            4335..4958
                     /gene="tesA"
                     /locus_tag="ACHJ12_001820"
     CDS             4335..4958
                     /gene="tesA"
                     /locus_tag="ACHJ12_001820"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4989617.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            4948..5757
                     /gene="ybbO"
                     /locus_tag="ACHJ12_001821"
     CDS             4948..5757
                     /gene="ybbO"
                     /locus_tag="ACHJ12_001821"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4989618.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTIRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            5818..6672
                     /gene="cnoX"
                     /locus_tag="ACHJ12_001822"
     CDS             5818..6672
                     /gene="cnoX"
                     /locus_tag="ACHJ12_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4989619.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(6735..7514)
                     /gene="fetB"
                     /locus_tag="ACHJ12_001823"
     CDS             complement(6735..7514)
                     /gene="fetB"
                     /locus_tag="ACHJ12_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4989620.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(7501..8178)
                     /gene="fetA"
                     /locus_tag="ACHJ12_001824"
     CDS             complement(7501..8178)
                     /gene="fetA"
                     /locus_tag="ACHJ12_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4989621.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            8324..9241
                     /gene="qmcA"
                     /locus_tag="ACHJ12_001825"
     CDS             8324..9241
                     /gene="qmcA"
                     /locus_tag="ACHJ12_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4989622.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9238..9696
                     /gene="ybbJ"
                     /locus_tag="ACHJ12_001826"
     CDS             9238..9696
                     /gene="ybbJ"
                     /locus_tag="ACHJ12_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4989623.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(9697..10104)
                     /gene="cueR"
                     /locus_tag="ACHJ12_001827"
     CDS             complement(9697..10104)
                     /gene="cueR"
                     /locus_tag="ACHJ12_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4989624.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10101..10198)
                     /locus_tag="ACHJ12_001828"
                     /pseudo
     CDS             complement(10101..10198)
                     /locus_tag="ACHJ12_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10229..11521)
                     /gene="ybaT"
                     /locus_tag="ACHJ12_001829"
     CDS             complement(10229..11521)
                     /gene="ybaT"
                     /locus_tag="ACHJ12_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4989625.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(11524..12456)
                     /gene="glsA"
                     /locus_tag="ACHJ12_001830"
     CDS             complement(11524..12456)
                     /gene="glsA"
                     /locus_tag="ACHJ12_001830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4989626.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            12718..15222
                     /gene="copA"
                     /locus_tag="ACHJ12_001831"
     CDS             12718..15222
                     /gene="copA"
                     /locus_tag="ACHJ12_001831"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4989627.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(15336..15677)
                     /gene="ybaQ"
                     /locus_tag="ACHJ12_001832"
     CDS             complement(15336..15677)
                     /gene="ybaQ"
                     /locus_tag="ACHJ12_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4989628.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            15815..16609
                     /gene="ybaP"
                     /locus_tag="ACHJ12_001833"
     CDS             15815..16609
                     /gene="ybaP"
                     /locus_tag="ACHJ12_001833"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4989629.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            16813..17292
                     /gene="ybaK"
                     /locus_tag="ACHJ12_001834"
     CDS             16813..17292
                     /gene="ybaK"
                     /locus_tag="ACHJ12_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4989630.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17329..18981)
                     /gene="ushA"
                     /locus_tag="ACHJ12_001835"
     CDS             complement(17329..18981)
                     /gene="ushA"
                     /locus_tag="ACHJ12_001835"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4989631.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            19199..20419
                     /gene="fsr"
                     /locus_tag="ACHJ12_001836"
     CDS             19199..20419
                     /gene="fsr"
                     /locus_tag="ACHJ12_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4989632.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            20657..22333
                     /gene="ybaL"
                     /locus_tag="ACHJ12_001837"
     CDS             20657..22333
                     /gene="ybaL"
                     /locus_tag="ACHJ12_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4989633.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22416..23720)
                     /gene="gsk"
                     /locus_tag="ACHJ12_001838"
     CDS             complement(22416..23720)
                     /gene="gsk"
                     /locus_tag="ACHJ12_001838"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4989634.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            23872..24831
                     /gene="aes"
                     /locus_tag="ACHJ12_001839"
     CDS             23872..24831
                     /gene="aes"
                     /locus_tag="ACHJ12_001839"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4989635.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(24828..25790)
                     /gene="hemH"
                     /locus_tag="ACHJ12_001840"
     CDS             complement(24828..25790)
                     /gene="hemH"
                     /locus_tag="ACHJ12_001840"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4989636.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(26026..26670)
                     /gene="adk"
                     /locus_tag="ACHJ12_001841"
     CDS             complement(26026..26670)
                     /gene="adk"
                     /locus_tag="ACHJ12_001841"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4989637.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(26851..28725)
                     /gene="htpG"
                     /locus_tag="ACHJ12_001842"
     CDS             complement(26851..28725)
                     /gene="htpG"
                     /locus_tag="ACHJ12_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4989638.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(28835..29440)
                     /gene="recR"
                     /locus_tag="ACHJ12_001843"
     CDS             complement(28835..29440)
                     /gene="recR"
                     /locus_tag="ACHJ12_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4989639.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(29440..29769)
                     /gene="ybaB"
                     /locus_tag="ACHJ12_001844"
     CDS             complement(29440..29769)
                     /gene="ybaB"
                     /locus_tag="ACHJ12_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4989640.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(29822..31753)
                     /gene="dnaX"
                     /locus_tag="ACHJ12_001845"
     CDS             complement(29822..31753)
                     /gene="dnaX"
                     /locus_tag="ACHJ12_001845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4989641.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(30425..30489)
                     /gene="dnaX"
                     /locus_tag="ACHJ12_001845"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(31882..32433)
                     /gene="apt"
                     /locus_tag="ACHJ12_001846"
     CDS             complement(31882..32433)
                     /gene="apt"
                     /locus_tag="ACHJ12_001846"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4989642.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(32586..32963)
                     /gene="ybaN"
                     /locus_tag="ACHJ12_001847"
     CDS             complement(32586..32963)
                     /gene="ybaN"
                     /locus_tag="ACHJ12_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4989643.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            33033..33560
                     /gene="priC"
                     /locus_tag="ACHJ12_001848"
     CDS             33033..33560
                     /gene="priC"
                     /locus_tag="ACHJ12_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4989644.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            33574..33735
                     /gene="rsmS"
                     /locus_tag="ACHJ12_001849"
     CDS             33574..33735
                     /gene="rsmS"
                     /locus_tag="ACHJ12_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4989645.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(33788..33886)
                     /locus_tag="ACHJ12_001850"
                     /pseudo
     CDS             complement(33788..33886)
                     /locus_tag="ACHJ12_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33947..37309)
                     /gene="mscK"
                     /locus_tag="ACHJ12_001851"
     CDS             complement(33947..37309)
                     /gene="mscK"
                     /locus_tag="ACHJ12_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4989646.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(37437..38084)
                     /gene="acrR"
                     /locus_tag="ACHJ12_001852"
     CDS             complement(37437..38084)
                     /gene="acrR"
                     /locus_tag="ACHJ12_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4989647.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            38226..39419
                     /gene="acrA"
                     /locus_tag="ACHJ12_001853"
     CDS             38226..39419
                     /gene="acrA"
                     /locus_tag="ACHJ12_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4989648.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            39442..42591
                     /gene="acrB"
                     /locus_tag="ACHJ12_001854"
     CDS             39442..42591
                     /gene="acrB"
                     /locus_tag="ACHJ12_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4989649.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         9900 a        11434 c        11231 g        10156 t
ORIGIN      
        1 tgaggctgag agagggcggg gtgtgtcagg ggaagataag gaagcggtgc gctgtttacg
       61 ggcctcttcc gcacgctgcg cctgcgtggt cagcaccaga cggaactccc gcccggcacc
      121 gtccgtcacg ccggtgattt ccccggccag gtcaccggcg gcctcacgcc ggtacgtcag
      181 cgtccgcccg aagcggtctg ccagcccggt aagcacccgg tacggcggca gcggcgctgg
      241 cagtacgtcc tcagcacccg gcaccagctc tgaccacccc agtatccacc acggcccctg
      301 tgcgctgttg gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg gcagcgacgc
      361 ccacagccgt gccagcgtat ggccgtccgg ctgcgttgcc ttaccgccac gcaccagcca
      421 cagggactcg ctgcggctgt acaccgcctc acccggcagc agcggctcaa agtgaatgct
      481 ccgtccgccg ttgtcgttga gtaccagtgc atcatcgcgt atctgtaagc ggatatcaga
      541 aggcgctttc cagccggggc caaaaatccc caccggtgca ggcgtcttcg tccggtagct
      601 gctgtaggtg cgggagagaa tgaacggcag cgggccgggc agcgcaaggt ccgtctcgcc
      661 gggcagcacc ttcgccccca gcagcggatt taccgggttg cccgaagtca tcccgcccgg
      721 acacaccgag cacgccacgc cagttggcgc gccaattctt acacctgccg aaccctggac
      781 aatgggaccg ccatactgag tcatatctcc ctgacgcgcc gctggttttc cgctcatata
      841 aagtgccctt ttagttattg ttcatatccc tgtgttagca acaaaaaaaa taaaatgaca
      901 cacaagacca gctaatgtat ttatatattt ttcacataaa cttatttgtt ctcaatcaac
      961 aacaactgtt attacaggtc actttcaata ttttattaaa ctaacatttt tagcacagat
     1021 gaattcatca tcaacaaatt cactatatgg ataatatcca ttcaacgcat atgatttatg
     1081 attttattag tctgagttat ttttgtaggg ctatatttaa aatcaataaa ctccaccgat
     1141 tcagattaat atcagcaata gaaagatagg aaatcaccaa ttcaataaca tcatcaatga
     1201 aacatattgt agaattaatt taaacaaggc gttccattca accagaacgc cccttattta
     1261 ctctcatcac cccgcaaact gcctgaacag cgccttaccc ttaaccagtc gcgcacccag
     1321 ccagccgccg aaaagcgaca gcagcagcgc tccgctgcac ggcagaacaa tccacaatcg
     1381 ccagtctggc tcccacggaa aatcaaacac tttcgcctgc aacactgcca gcgccgtttc
     1441 cgcaccaatt gcggccacca ggccggaaac aaacccaagc atggcgaact cacaccacaa
     1501 cgtggtacgc agcagttttt tccccgcacc gagtgtgcgc cacaccacca gctcctggtg
     1561 gcgctgacgc atacccacct gcacctgtgc cagcaacagc aacataccgc aggcggtgac
     1621 cagcaccacc atcacctcca gcgcccgact tacctgctcc agcacctgac cgacctgttt
     1681 taaaatcgcg ccaatgtcta acaggctaat ggtcgggaac tggcggttga gttgcgtcaa
     1741 catgccgttg ccattctccc agcggaaact ggtaagccag ctctgcggtt gcccgtctaa
     1801 tgccccttcc gggaaaataa aatagaaatt aggccgcaga ctttcccagt ccactttgcg
     1861 caggctggtc actttagcgc ggaactcctg ggtatcgccc ataaaagtca cggtatcacc
     1921 gagggcaacc tttaagcgtt tcgccagccc ctcttccatc gacacttcat cggcttttgg
     1981 cggccagtta ccggcgacaa tcggattatg atcgggccgc gtattttgcc aggtaagatt
     2041 gagttcgcgg ttaagcgcct catcttcatt accttctgtc ggcttatcgt taatcgccgt
     2101 cagccgcgcc cgcaccaccg gataaaacga ttccgggact atctgatgtt ccgcgaggaa
     2161 cgcttttagc ggtgctacct gttctgtggc gatgttaatt aaaaagtagt tcgggctttc
     2221 tggaggtagc tgctgctgcc agcggtcgag cagatcacca cgcaacacca gcagcagtgc
     2281 cagcagcata aaggagagcg aaaatgccga aagctggctt aacgttgacc acggctgacg
     2341 taacaggcgg ctgaccgcca ggcgcagagg cagcgatttc agcgtcatgc ggcgaagtac
     2401 attcagcagc atccagccca gcacaccgca cagcaaagcc agtactaccg cgcccgccag
     2461 caccgcccag agcagcatac tgccccccat taatccggcg agcagcagca caaccactac
     2521 actgacaatc ggcagataaa acttcagcgg ccagacgttc gctaccacat cattacgtaa
     2581 tacgcgtaaa ggctgcgttg ccagcaacaa gcggtatggt cgtagcccca ccagcagcga
     2641 gatgaccgtc atggtgccca gcgcccatag ccacggccag aggctggctg gcggtagtgc
     2701 agcaggcaga acgggcttga gcagcaccat caacacgttt tcgaacaaca ggcctatcgc
     2761 cccaccggta acggctgaaa gcgtcagcac catcaaccac tgaccgacga ttagcttacg
     2821 cagttgcgct cgccctgccc ccagcgtttt gaggatcgcc accagatcgt agcgactgcg
     2881 acagtaatga ttcatcgcta ccgccactgc tgccactgcc agcagcaagg tcagaagcgc
     2941 cgaaagcagc aggaactgtt gcgagcgttc catcgatcgc cccagcgcgc cttcgtcctg
     3001 ttccagaccg taccagcgtt gttcgggttt aagctgaggt aacaaccatt tctcatagct
     3061 gtcgagctgg ttctcactgc cgccgaattt atagcgccag gtgacccgac tccccggctg
     3121 cacggctccg gttttatcga catccgccag attcatcatc agacgcggag ccatctggaa
     3181 ggggttaaaa ccggaatccg gttcctgaat cacttctccg gcaatccgca aggtggcatc
     3241 gcccacgtca atggtgtcgc ccgttttcag gttaagcagt gccatcaggc gtggggccag
     3301 caatacgctg cccgcctgcg gtttcaggcc agggggattg gtttgcagat cgccatacat
     3361 cgggtagata tcatccaccg ctttaacgtt cgccagctgc ggtgtgtcgc ctgcaaatgt
     3421 catggtggcg aaagtcagct gcttgccgac tttcaggccg cgcttttgcg cttcctccag
     3481 ccacgcttgc ggcacttcgc gcgaactgcg caacgcccga tcgcccgcca taaattcacg
     3541 gctttgctgg cttaagcctt tttccatgcg atcgctgata ttacccagcg ccagcacgca
     3601 ggccaccgcc aggcttaacg ccagccagac aatcaatagc gaaggcgagc gccattcgcg
     3661 ccagaaccag cgtgcaatca tgcttcctcc tgcaactgcc cgttcaccag ccgtaagcag
     3721 cggtcgcagc gtgccgccag ttgcaggtcg tgggtcacca taatcaacgt ggtgccatgc
     3781 tcacggttga gggaaaacag caggtcggca attttatcgc cggtctggcg gtcaaggttg
     3841 ccggtgggtt cgtcggcaaa cagcacatca ggtcgaccgt taaaggctcg tgccagcgcc
     3901 actcgttgct gttcaccgcc ggaaagctgt gccggaagat gatccagacg tttacccaga
     3961 cctaactgtt cgagcaacgc tttcgctccg ttacgacttt ccgcgctact ctcaccgcgc
     4021 agcagcgccg gaagctcgac gttttccagc gcgttaaggg taggaattaa cataaatgac
     4081 tgaaaaacaa agccgacgtg cttcgcgcgc aactttgccc gcgcttcttc gtccatatta
     4141 tgtagcggtt gtcccaccag actcacttcg ccactgctgc cgtcatcaag cccggcgagg
     4201 atcgccagca aggttgactt acccgatccc gactcgccca ccagtgcgat ggtctcgcca
     4261 cgtttgacaa ccagctcaac tccggtgagg atggagagtt catgctcccc ctgaccgacg
     4321 gacttcttaa gatgatgaac ttcaacaatg ttttccgctg gcatttgccc ttcctgttcc
     4381 tggtcctgtt aaccttccgt gccgccgcag cggacacgtt attgattctg ggtgatagcc
     4441 tgagcgccgg gtatcgaatg tctgccagcg cggcctggcc tgccttgttg aatgataagt
     4501 ggcagagtaa aacgtcggta gttaatgcca gcatcagcgg cgacacctcg caacaaggac
     4561 tggcgcgcct tccggctctg ctgaaacagc atcagccgcg ttgggtgctg gttgaactgg
     4621 gcggcaatga cggtttgcgt ggttttcagc cacagcaaac cgagcaaacg ctgcgccaga
     4681 ttttgcagga tgtcaaagcc gccaacgctg aaccattgtt aatgcaaata cgtctgcctg
     4741 caaactatgg tcgccgttat aatgaagcct ttagcgccat ttaccccaaa ctcgccaaag
     4801 agtttgatgt tccgctgctg ccctttttta tggaagaggt ctacctcaag ccacaatgga
     4861 tgcaggatga cggtattcat cccaaccgcg acgcccagcc gtttattgcc gactggatgg
     4921 cgaagcagtt gcagccttta gtaaatcatg actcataaag caacggagat cctgacaggt
     4981 aaagttatgc aaaaatcggt cttaattacc ggatgttcca gtggaattgg cctggaaagc
     5041 gcgctcgaat taaaacgcca gggttttcat gtgctggcag gttgccggaa accggatgat
     5101 gttgagcgca tgaacagcat gggatttacc ggcgtgttga tcgatctgga ttcgccagaa
     5161 agtgttgatc gcgcagccga cgaagtgatc gccctgaccg ataattgtct gtatgggatc
     5221 tttaacaatg ccggattcgg catgtatggc cccctttcca ccatcagccg cgcgcagatg
     5281 gaacagcagt tttccgccaa ctttttcggc gcacaccagc tcaccatacg cctgttaccc
     5341 gcgatgttac cgcacggtga agggcgtatt gtgatgacat catcggtgat gggattaatc
     5401 tccacgccgg gtcgcggcgc ttacgcggcc agtaaatatg cgctggaggc gtggtcagac
     5461 gcactgcgca tggagctgcg ccacagcgga attaaagtta gcctgatcga acccggtcct
     5521 attcgtaccc gcttcaccga caacgtcaac cagacgcaaa gtgataaacc agtcgaaaat
     5581 cccggcatcg ccgcccgctt tacgttggga ccggaagcgg tggtggacaa agtgcgccat
     5641 gcttttatta gcgagaagcc gaagatgcgc tatccggtaa cgctggtgac ctgggcagta
     5701 atggtgctta agcgcctgct gccggggcgc gtgatggaca aaatattgca ggggtgagtt
     5761 gaagcgcgcg cttaagcccc catgtcaaag aaaatatcga caacagagag cgactccatg
     5821 tccgtagaaa atattgtcaa cattaacgaa tctaacctgc aacaggttct tgaacagtcg
     5881 atgaccactc cggtgctgtt ctatttttgg tctgaacgta gccagcactg tttgcagtta
     5941 accccaattc tggaaagcct tgcggcgcag tacaacgggc aatttattct ggcgaagctg
     6001 gactgcgacg cggagcagat gattgccgcc cagtttggtc tgcgcgcgat tccgaccgtg
     6061 tatctgttcc agaacgggca accggtagat ggcttccagg ggccgcaacc ggaagaggcg
     6121 atccgcgccc tgctggataa agtgctgccg cgcgaagaag agctgaaagc gcagcaggcg
     6181 atgcaactga tgcaggaagg caattacacc gatgccctgc cattgctgaa agacgcctgg
     6241 cagttgtcga atcagaacgg ggagatcggc ctgctgctgg cagaaacgct gattgcgctg
     6301 aaccgttctg aagatgcgga agcggtgctg aaaaccattc cgttgcagga tcaggacacc
     6361 cgctaccagg ggctggtggc gcaaatcgaa ctgctgaagc aggcggctga tacgccggaa
     6421 attcaacagt tgcaacagca ggtggcggag aatccagaag atgccgcact ggcgacgcaa
     6481 ctggctctgc aactgcacca ggttgggcgc aatgaagagg cgctggagtt gctgttcggg
     6541 catctgcgta aagatctcac cgccgcagac ggtcagacgc gtaaaacgtt ccaggagatc
     6601 ctcgctgcgc tgggtacggg tgatgcactg gcgtcgaagt atcgccgcca gctgtatgca
     6661 ttgttgtatt gatattccgt tgatcggcac atgaaccaca acgccggatg cgatgctgcg
     6721 catccggcat cgcatcattt cttcttcaat tgcgtcacca ccaactggtg gcgcgaatta
     6781 taaaacttac gataggttaa atagcaggca ataatggtcg acaagctggc ggttgagagc
     6841 agcataaagg tcaccataat ctgatattta atcgccttca ccggatcaat cccggcaaat
     6901 atcagcccgg acatcattcc tggtaaactc actaagccaa ccgtttttgc tgaatcgacc
     6961 gtcggaatta aagccgcgcg aatactgtcg cgaatcaata tcgctgaagc ctgcttcggc
     7021 gtcgcaccaa gactcagttt ctcctgaatc tgttgctgtt cgctaatgac ccgttgccct
     7081 aaattgttgt aacacaaccc caccgctacc atggcgttac cggcaatcat cccggcgata
     7141 gggatcacct gcatcgggat aaattcaatc gacccagaga gaatcagcac cgccagggta
     7201 attcccgccc cgaccgtaat ggcaataaac gatgagataa aagctttagc aatatattta
     7261 ctgcgttttt gcgcgttcca cgccgcatta aagcagataa ataacaccat caataatgtc
     7321 aggctggcat catccacgct gaaaatatac ttcagcacat agccgacaat aatcagctga
     7381 attatcgctc gcccgacgct ccagaaaata tctttctcca gcgccagttt ttctttatgg
     7441 ctaattaaga ttgccaccac caccagcatt aatgccagtg ccaatgattc gttagtaata
     7501 ttatgcgagt tcatagcgtg cttcctgcat ttctccggca tgcggttgca gtgtaatcac
     7561 tttatccgca tgattaattt cgtctttatc gtgtgtcacc cacagcacgg caatattttg
     7621 ctcgcgcaca taacgatgga tcatctcatt aacgttatgt ttattacttt catccagcgc
     7681 actggtgatt tcatccagca ataaaacctt cggcataaat tgcaggttac gaatcagtga
     7741 gatgcgttgt ttttcaccac cagatagctc ggcgatattt ttcgtcaaaa tgctgtccgg
     7801 caaagcgaag cgttcgagaa aatcgagaaa aatggccggg tcaggctgct ggttacggat
     7861 ctgccaggga aagatcagat tatcgtatac cgtatcgcca aacagcgtcg gcgtctgggc
     7921 gcagtaagag acttgctggc ggtagatttc tggctttagt gtgctgacat cctcaccttc
     7981 aaacagtaac gttccgctgg ttgggctgat caatgaagca actattttta gcagcgtact
     8041 tttgccacaa ccggaaggac cggtaattaa cttaaattcg ccagcacgca gcgaaaaatt
     8101 gatgttatta agaatcttcg catcacccgc cagatatcct acgttttgta gctgaagcaa
     8161 aggactattt tcctgcatca ctgttccctt tttctgattt ttactaaaaa cagtttatcc
     8221 ttcgcaggaa taagggggaa ctctctttca gtaatcaggt ataatttgtt taatcaagca
     8281 gcaattttgc tgattatgga cggtacaaca ggaggttttt ccgatgctta tctttatccc
     8341 gattctcatt tttgtcgcgc tggtcattgt cggcgcgggc gtcaaaatcg tgccgcaggg
     8401 ctatcaatgg acggtagaac gttttggtcg ctataccaaa acgttacagc cggggctcag
     8461 tctggtggtg ccgtttatgg atcgcattgg tcgcaagatc aatatgatgg agcaagtgct
     8521 cgatatccct tcccaggaag ttatctcgaa agataacgcc aacgttacca tcgacgcagt
     8581 ctgttttatt caggtgattg acgcgccgcg cgcggcttat gaagtcagca atctggagct
     8641 ggcgatcatc aacctgacca tgactaacat ccgtaccgtg ttgggttcaa tggaacttga
     8701 cgaaatgctc tctcagcgcg acagcatcaa ctcacgcctg ctgcgtattg tcgatgaagc
     8761 caccaacccg tgggggatta aagtcacccg tattgaaatt cgcgacgtgc gcccaccggc
     8821 agagcttatc tcttcaatga acgcgcagat gaaagcggaa cgtaccaaac gcgcttacat
     8881 ccttgaagcg gaagggatcc gtcaggcgga aatactcaaa gcggaaggtg aaaaacagtc
     8941 gcaaattctg aaagcggaag gtgaacgtca gtcggcattc ttacaggccg aagcgcgtga
     9001 acgttctgcc gaagcagaag cccgcgccac caaaatggta tctgaagcca ttgcctccgg
     9061 tgatattcag gcggtgaact acttcgtagc gcagaaatac accgaagcgt tacagcagat
     9121 cggttcctcc agtaacagca aagtagtgat gatgccatta gaggccagca gcctgatggg
     9181 gtcgattgcc gggattgccg agctggtgaa agacagcgcc aacaaacgga ctcagccatg
     9241 atggagttaa tggtcgttca tccacatatt ttctggctca gtctcggcgg gttgctgctg
     9301 gcagccgaga tgctgggcgg aaatggttat ttgctgtgga gcggcgtgtc agcggtgatt
     9361 accggcctgg tggtctggct ggtgccgctg ggttgggagt ggcaaggggt gatgtttgcc
     9421 atcctgacgc tgctcgccgc ctggctgtgg tggaaatggt tgtcgcggcg ggtgcgcgaa
     9481 caaaagcaca gcgatagtca tttaaaccag cgcgggcagc agctgattgg ccgacgtttt
     9541 gtgctggaat ctccgctggt caacgggcgc ggtcatatgc gcgtcggtga cagttcatgg
     9601 cctgtcagcg ccagcgagga tctcggtgca ggtacgcatg ttgaagtcat tgcgatagaa
     9661 gggataaccc tacatatccg agccgtctcg tcttaatcac cctgcccgat gatgacagca
     9721 gccggagaga ttttcgataa tcgggcagtc ggcgctgtca tcgccagggc aggcattcgc
     9781 cagtgccagc agctggtcgc gcatggattg cagctcctca atgtgtcgtt cgatctccgc
     9841 caccttctcc agcgtgcgcc gtttgacgtc ggcgctgtgc cgctgcgggt cgttaaacag
     9901 attcaccagc tcgccgctct cttccaggtt aaagcccacc tgccgtgcct ggcgcagtaa
     9961 ggtcagttcg ttgagatgct gctgcgtgta ggtgcgataa ccgttttcgc tgcgcatcgg
    10021 cggcgtcacc agccccttct cttcatagaa gcgaatggct ttgctggtca ggccggtaat
    10081 ttttgctaca tcgctgatgt tcatcgttcg cgcaacgcct cctgtgcttt gttaaagggt
    10141 ttcaacagat attcaagcac gtttttttgc cagtgcgaat atcgaccgcc accacaataa
    10201 tacaggaagt actgcgcaca gagagatgtt atacggtttt attgcgcttc atgaccattg
    10261 ccacaataag gctgagtaag agtgagccaa taatccatat caacgcacgg ctaccctggg
    10321 accacagact gtagataaag ccaacgatca ccaacaacat caccaacgta ccaacgataa
    10381 gaatcggcaa cgaggcgtga atatcatgac gcaggcggat cgccaccaca aacaccgcca
    10441 ggtagcaaat caaaaaggtg gcgctggcaa cgctggcgag tgagcctaaa ttcagtgccg
    10501 ccgtcatcag cataatcaac accacgacaa tgatgttgcc ccaggtactc tgccgccaca
    10561 gggatttatt cattagcttc ggtagttcgc gttcgctgcc catgttgtcc atgatgttaa
    10621 acacggcgaa caggttcgcg ttaatggctg aagccgtcgc cagtaaagcg ccgatgacga
    10681 cgatcacata acccacatgc ccgagcagcg gagaagctgc ctgcgctacg gcggtatcgg
    10741 catatttttc taactctaat gccgatacat cgctaagcaa aaccagcgcc agcgagatat
    10801 aaagcaacgt ggtaacgcca attgccacca gaaacgcccg tggcataatg acctgcggat
    10861 ctttcacttt atccgccgcg ttcgccatca tgccaaagcc cgcataggca aggaaggtta
    10921 tcccgataca ggagaagaac gcaccggagc tggggggcgc agagacagaa atatgcgccg
    10981 gttgcaacga ccagacaccg gcaataatca gcaataacag gatcatcatt ttaatgccga
    11041 cgaggatcac ttccagccgc cctaccgcat ggttgcttaa ggagttgaaa agcgtcatca
    11101 ccgcaatgat ccccaacgcg tagagcaaaa taaggtgctc ctcctggctg ccttcatgca
    11161 aaaactgcac ggcataagcg ccaaaagcac gggcgaccat ggcgatgctc accgccagcg
    11221 tcaacaggta cagtaacgag agcgccagcg aaaagacgcc gttgcctaat ccgcgacgaa
    11281 agaagtcgat aatgccgcca ttgctgggat agctcgcccc cagacgcgca taggcataac
    11341 cggaaaacat cgccacaata ccgccaaaag caaaggcgac ccaggtcgag gcttccatta
    11401 gcaatgcagc ctgccccagc agcgcgaaga tccccgcccc caccattgcc ccaatgccga
    11461 tggaaacgac gttccataga ccgagaggtt tgttaccgtt attaccttcc gtgttcatca
    11521 tgatcagccc ttaaacacgt tatagccgag ttgcttagcg accgatgcca ccattttttg
    11581 accgcgaaca ctgttgcctt cttcgtccag cggtggtgag aacgcggcaa ttcccatcac
    11641 gccagggacg accgccagaa taccgccacc tacaccgctt ttgcccggta aaccaacgcg
    11701 atacgcccag tcaccggagc gaccatacag cccttccatc atcatttcgg ccagaatgta
    11761 cggcacgttg tcggcctgaa gaacgcgttc atgcgtcagc ggattcacac cccccgccgc
    11821 cagcgtcgcg ccaagtgttg ccagttcaac agtattgagg agcgtggagc actgacgggt
    11881 gtacacgtca caggcttcca ttgcatcaca atagagatat ccggcggagt acagcagcca
    11941 ggcaatggcc cggttatgga agttggttgt ttgttccgac tggttgactt cgtcagagag
    12001 cgctacctgc tcaccagcca gttgctgttg gatatgtaaa attcgctgcc agcgttgttc
    12061 aacattttca gcgttaatca ggctggtggt ggcaatggcg ccagcattta ccagtggcga
    12121 aagcggtttg ccgccatgca actctaaggc gataactgag ttaaagggca atccggtcgg
    12181 gtcagcgcca attttgtcct gtaccgcctg cgggccgaca tcctctaacg caagggctaa
    12241 cgtacagact ttcgagatgg attccagcgc aaagcggtaa tcactatcac ccgcgcgata
    12301 gatgttgcca tcgcaggtca caatagccac tgctgccagt tgactaggta cattcgccag
    12361 aaagggaatg tagtcggcat tttgtccgcc gttaagtgag tgaaattggg tgtaagcctg
    12421 atccactgcc tgctgtaatt tgtttgcatc taacatcttt tgttaactcc tttttataga
    12481 tgcgggaggt aattcctcac cccggtgccg attttcaggc atcttgattt aacttagcac
    12541 ccgcaactta acttcaggaa aacaaagaga taaatgtcta atcctgatgc aaattgagcc
    12601 aattttttaa tctttacgga cttttactcg cctggtttat taatttcttg accttcccct
    12661 tgctggaagg tttaaccttt atcacagcca gtcaaaactg tcttaaagga gtgttttatg
    12721 tcacaaatta tcgacctgac cctggacggc ctgtcctgcg gtcactgcgt taaacgcgtg
    12781 aaagaaagtc tcgaacagcg cccggatgtg gaacaagcgg atgtgtctat cactgaagcg
    12841 cacgttaccg ggactgccag tgcagaacag ctgatcgaaa ccatcaaaca agcgggttat
    12901 gacgcatctg taagccaccc aaaggctaaa ccgctggcgg agtcatcaat cccgtcggaa
    12961 gcactgacag cggtttctga ggcgcttccg gcagcgaccg ccgatgacga tgacagccag
    13021 cagttgctgc tgagcggcat gagctgcgcc agctgtgtca cccgcgtaca aaatgcgctg
    13081 caaagcgtac cgggcgtcac tcaggcacgg gtaaacctgg cggagcgtac tgcgctggtg
    13141 atgggcagtg cctccccaca agatttagtg caggcggtgg aaaaagcggg ctacggtgcg
    13201 gaagcgattg aagatgacgc taaacgccgc gagcgccagc aagaaaccgc cgtagctacg
    13261 atgaagcgct tccgctggca ggcaattgtt gcactggctg tgggtatccc ggtaatggtc
    13321 tgggggatga tcggcgataa catgatggtc accgctgaca accgcagctt gtggttagtt
    13381 atcggcctga taaccctggc agtgatggtt ttcgccggcg gccattttta ccgcagtgca
    13441 tggaaaagcc tgctgaacgg tgcagcgacg atggatacgc tggtggcgct gggaacgggt
    13501 gtggcgtggc tctattcgat gagcgtcaac ctgtggccgc agtggttccc gatggaagcg
    13561 cggcatcttt actatgaagc cagtgcgatg attatcggtc tgattaatct cggccatatg
    13621 ctggaagcgc gcgcacgtca gcgttcttct aaggcgctgg agaaattact cgatttaacc
    13681 ccaccgacgg cgcgcctggt tactgacgaa ggtgaaaaaa gcgtgcctct ggcggaagtg
    13741 cagccaggta tgttgctgcg cctgacgacc ggcgatcgcg tgccggtaga tggcgagatt
    13801 acccagggcg aagcatggct ggatgaagcg atgctgacgg gcgaaccaat cccgcagcaa
    13861 aaaggcgaag gcgaaagcgt ccatgccggg acagtggtac aggacggtag cgtgctgttt
    13921 cgtgccagtg cggttggcag ccataccacg ctgtcacgga tcattcgcat ggtgcgccag
    13981 gcccagagca gcaagccaga aatcggtcag ctggcggata aaatctcagc cgtatttgta
    14041 cctgtggtgg tggtgattgc gctggtcagt gcggcaatct ggtacttctt tggtccggca
    14101 ccgcaaattg tttataccct ggtgattgcc accacggtac tgattatcgc ctgtccgtgt
    14161 gcgctggggc tggcgacgcc gatgtcgatt atttccggcg tcgggcgggc ggctgagttt
    14221 ggcgtgctgg tgcgggacgc cgacgcgctg caacgcgcca gtacactcga taccgtcgtg
    14281 ttcgataaaa ccgggacgct gaccgaaggg aagccgcagg ttgtcgcagt gaaaacgttt
    14341 gctgatgttg atgaagcgca ggcattgcgt ctggcggcgg cactggagca aggttccagc
    14401 catccgctgg cacgggcgat cctcgataaa gcaggtgata tgcagctgcc gcaggtcaac
    14461 ggtttccgca cattgcgcgg gctgggcgtg agcggtgaag ctgaaggtca tgcgttattg
    14521 ctgggcaatc aggcgctgtt aaatgagcaa caggttggta ccaaagctat cgaagcggag
    14581 attactgctc aggcatcgca aggagcaacg cctgtgctgc tggcgattga cgggaaagca
    14641 gtagccctgc tggctgtacg cgatccgttg cgtagtgata gcgtggcggc gctgcaacgc
    14701 ctgcataaag cgggatatcg tctggtgatg ttgaccgggg ataacccaac caccgccaat
    14761 gcgatcgcca aagaagctgg aattgatgaa gtgattgccg gagtgctgcc ggacggtaaa
    14821 gccgaagcga tcaaacgtct gcaaagtgaa ggacgtcagg tggcaatggt gggcgacggc
    14881 attaacgacg cgccagcgct ggcccaggcg gatgtcggca ttgcgatggg tggcggcagt
    14941 gatgttgcca ttgaaaccgc ggcgattacc ctgatgcgcc atagcctgat gggcgttgcg
    15001 gatgcgctcg cgatttcccg cgcaacgctg cgcaacatga agcagaacct gctcggtgcc
    15061 tttatctaca acagcatcgg tattccggtc gccgccggta ttttgtggcc gtttactgga
    15121 acactgctta acccggtagt tgccggagcg gcaatggcgc tctcgtcgat taccgtagtg
    15181 agtaacgcca accggttgct gcggtttaaa ccgaaggaat aagtacgtca ttgcggatgc
    15241 gctgctttag ggcggcgcat ttggcatcga cgcatgactg ccggatgcgg cgtaaacgcc
    15301 ttatccggcc tacgagatga agcgatgttt tggccttacg cgaccttttt tgcacgctct
    15361 tcacggcgtg ccaaatattc agccactgtt tcaatccgtc ccaattcttc ctgggtgcgc
    15421 atgttgtttt caacttccca aagatcaact gccgcctgga ggtttagcct aaaatcgact
    15481 gtggtatcaa aaactttcgc cagacggaat gccatctcag tggtgagttt acgattgtta
    15541 ttgatcagtg cactgacgct attacgatga acatgcagca actctgctaa ctcattgatt
    15601 ttcaaatcga gcggttccag atattcatag agaagaatat ctccgggtgt cgtcggtttt
    15661 cttgttgcct gtttcatggt ttttaactgt tgcttccctg tgaatagcga tgcaagaaca
    15721 tactgaatca tacaaacaca ttaaacaagt ccccaactcc atttgcgcca tccctccccg
    15781 cgcgctagca tgatatttca caaagggagg tcgtatggat ctgttgtacc gggtaaaaac
    15841 gctttgggcc gcgctgcgcg gtaatcatta ctcctggcct gccatcgata tcaccctccc
    15901 cggcaatcgc cattttcatc tgattggcag tattcatatg ggtagccacg atatggctcc
    15961 cctgcccacc cgtttgctca aaaagctcaa aaacgccgat gcgctgatcg tcgaggcgga
    16021 tgtttccacc agcgatacgc cttttgctaa tttgcctact tgcgaggcgc tggaagagcg
    16081 cattaacgag gagcaactcc aaaatctgca gcacattagc caggagatgg gcatttctcc
    16141 gtcgctcttt tctactcaac cgctgtggca aatcgcgatg gttcttcagg cgacgcaggc
    16201 acaaaaactg gggctgcggg cagaatacgg tatcgattac cagttattgc aggcggcgaa
    16261 gcaacaacat aaacccgtga ttgaactgga aggggctgaa aaccagattg ccatgttgct
    16321 ccagctccct gacaaaggac tggcgctgct ggacgatacg ctgacccact ggcataccaa
    16381 cgcacggttg ctgcaacaaa tgatgagctg gtggctgaat gcaccgccgc aaaataatga
    16441 tataacgctg cccaatacgt tcagtcagtc gctgtacgat gtgctgatgc atcagcgaaa
    16501 tctcgcctgg cgggataaat tacgcgccat gccgccgggg cgatatgtgg tcgcggtcgg
    16561 tgcactacac ctgtatggag aagggaattt accgcaaatg ttgcgctaaa aaaatggcca
    16621 atatcgctat tggcccgtca aagaggaatt tcattttttt attattatgc cgtcacttta
    16681 agcgacggtg taactcgatt gtcgctcaat ctcaccatcc tgccaatact cttaggcaag
    16741 atgactcttt tttttggacc gccatcaggc gtatgcttgc ttcgtttttt tgtttaaagc
    16801 aggaaaatta ccatgacacc cgcagttaaa ttactcgaaa aaaacaagat ttcgtttcaa
    16861 atccatacct acgagcacga tccggctgaa accaattttg gcgatgaagt cgtcaaaaaa
    16921 ttaggtttga atccggatca ggtctacaaa acgctgctgg tggcagtgaa cggtgatatg
    16981 aaacaccttg ccgtggccgt tacgccggtc gcaggtcaac tggatcttaa aaaagtagca
    17041 aaagcgctgg gtgccaagaa agttgagatg gccgatccga tggccgcgca gcgttcgacg
    17101 gggtatctgg taggggggat tagcccactg gggcagaaaa aacgtctgcc aacaattatc
    17161 gacgcccctg cacaagaatt tgccactatt tatgtttccg gcggcaagcg cggactggat
    17221 atcgaactgg cggcaggcga tctggcaaag atcctcgatg ccaaatttgc tgatatcgcc
    17281 cgccgcgatt aagcattgtg ccggatgcaa acatccggca ctttcggatt actgccagct
    17341 cacctcacct ttcggttcat aaacactcac atccagcggc gagcttttct ggatatacgc
    17401 tttcagcact tcggcatcaa taaagccggt attcacatag cccggtttgt tatcaaggcg
    17461 cggatatcca tcaccgccgg tggcattgaa gtttaatgtc gccatacggt aagttttcgc
    17521 cggatcgacc ggttcgcctt tgattttaag gtcgttcagt ttgccgtctt tcgccacaaa
    17581 gctaacgttg gcaaattgcg ggtaggcacc tgaatctggc ttcatctgcg cgacggcggt
    17641 caggtaatca atcacctctt taccggtcat gtcggcatac accaccacat tgccgaatgg
    17701 ctgcactttc agcacgtttt tatagctgat atcgcctgct tcgatagaat cacgaattcc
    17761 gcctccgctc atcaccgcaa agtcggcacc agtgcgatcc atttgggctg ccagaatcaa
    17821 ccgccccata ttagtctgta caaaacgcac tttgtcacga tcgccttcca gacgaccatt
    17881 ggtttcgcct attttcactt ccagctgcgc tttgcctttg ttctggaacg gtgataacag
    17941 cgagatcatt tgctggtttt cagcgatttc aggagtgtaa agcacgcgct cgcttttccc
    18001 gtcttcccag gtcactttct tcttcaggtt caccggaatc agctggtagt taaccatttt
    18061 catttcgcca ttacgaaact caaaatcagc ccgtcccacg tatttgcccc actcatgcgc
    18121 ctgcacaatc cagatgccgt tttgttgatc tggcttgcat ggcgtacccg gcacgtaatc
    18181 gacctgtttt ttgttttctg ccgccatgca gaccggatct tgcgagtgac caccgacgat
    18241 catcgccagc gatccggcag gcagcgcgcg tgccatctcc acatcgcccg gtgcgttaga
    18301 gccgtgctca ccattatcgt aatgccccat atgggtcgcc gcgataataa tgtctggctt
    18361 ttctgtctgt tgcagctcct gaatcaccag cttcgcttca tcggcgggct tacgaaattc
    18421 gacatcagtg aagtattccg ggttaccaat ttttgctgtg tcatcggttg tcagcccaat
    18481 gacggcaatt ttcagatcct gacgcttaaa cagcgcccac ggtttaaaca ggcgctcgcc
    18541 agtacttttc tggtagatat tcgcggaaag caacgggaac ttggcccact tttcctgctg
    18601 gcgtaatacg gtgagcggat tatcaaattc atgattaccg atcgccatcg cgtcatagcc
    18661 caccagattc ataccgcgaa aatcaggttc ggcatcctgt aagtcagact cgggcacgcc
    18721 agtgttaatg tcgccaccgg aaagtagcag cacgctaccg ccttcagccg caacctcttt
    18781 gcggatacca tccaccagcg ttttttgcgc cgccagacca tattcgccat attcattgcg
    18841 ccaaaaatgc ccatgatgat cattggtatg cagaactgta attttgtagg ttttatcctg
    18901 ctcatacgcc agagcagttt cactcgccag tgtaaatgtg gttaacagcg ctaacgccac
    18961 gccccgctgc aataatttca tacttctctc cctgacctga tttcaacgtg ttgaaaaata
    19021 gtatgacgcg acatcataga caagtctgcg tccggtgaag ataaatctgc atccagatta
    19081 gaaattctgt attcggcgtt gagacttcct tcaggcgacg catacaggta tgttagtcgg
    19141 gtaataatca caaataagat cattctttac atcgcaaccc tgaaaaagtg actttcctat
    19201 ggcaatgagt gaacaacccc agcctgtggc gggcgcggct gcgtcaacga ccaaggcccg
    19261 aacatcgttt ggtattttag gtgctatcag cctctcacat ctgctgaacg acatgatcca
    19321 atcgctgatt ctggcgattt atccgctgct tcagtcagaa ttttctctga catttatgca
    19381 gattggcatg ataaccctca ccttccagct cgcctcttcg ctactgcaac cagtggtcgg
    19441 ctactggacc gataaatatc cgatgccgtg gtcgttgcca attggcatgt gctttacctt
    19501 aagtggtctg gtgctgcttg cgctggcggg cagttttggc gcagttctgc tggcggcggc
    19561 gctggtcggt accggttcat cggtctttca tccggaatct tctcgcgtag cccgtatggc
    19621 ttccggcggg cggcatggcc tggcgcaatc tatctttcag gtcggcggca actttggcag
    19681 ttccctggga cccttgctgg cggcggtgat tatcgcgcct tatggcaaag gcaacgttgc
    19741 ctggtttgtg cttgcggcac tgctggcgat cgtggtgttg gcgcaaatta gccgttggta
    19801 ctcggcacag caccgaatga ataaaggaaa atccaaagcg acgataatca atccactgcc
    19861 gcgcaacaaa gtggtactgg cagtcagcat tctgttaatc ctcattttct cgaaatattt
    19921 ctatatggcg agcatcagca gctattacac cttttatctg atgcaaaaat tcggattatc
    19981 tatccagaat gcccagcttc atctgtttgc cttcctgttt gccgttgcgg caggtacggt
    20041 gatcggcggg cctgtagggg ataaaattgg acggaaatat gtgatttggg gctctatcct
    20101 cggcgttgcg ccgtttacgc tgattttacc ctacgccagc ctgcactgga cgggggtttt
    20161 aacggtgatt attggattta tcctcgcttc ggcattctct gccattctgg tctacgctca
    20221 ggagctactt ccgggacgta tcggtatggt ttctggactc tttttcggtt ttgcttttgg
    20281 catgggaggt ctgggagcgg cagttctggg gcttatcgcc gatcacacca gcatcgagtt
    20341 agtctataaa atctgtgctt tcctgccact attggggatg ttgaccatat tcctgcctga
    20401 taaccggcat aaagactgat ttcattgcag ccaaaggcaa actttggctg catcgtttta
    20461 cagtcgccat aagcctttcc tctgttaaac cgccttctgt tttccgatcc gtgctttttt
    20521 tgcagctaat tccatttttc tgcaaaattc aataattatt catgaacatc atcaatgaaa
    20581 ttgtcataaa ctattacctg taattttggt tttcccggcc aaaaatggac aataaccacc
    20641 aggaaaagga gacggaatgc atcacgccac cccgcttatc accaccattg ttggcggcct
    20701 tgtgctcgcc tttatcctcg gcatgctggc caataaacta cgtatttctc ctctggtggg
    20761 atatctgtta gcgggtgtgc tggcaggacc attcactccg ggctttgttg ccgataccaa
    20821 gcttgcgccg gaactggctg aactgggcgt cattctgttg atgtttggcg tcggtttgca
    20881 cttttcgctg aaggatttga tggcggtaaa ggccatcgcc attcccggtg cgatcgccca
    20941 gatagccgtg gcgacgctgc tgggtatggc gctctctgcc gtgctgggct ggtcgttaat
    21001 gaccggtatc gtgttcggtt tatgtctttc caccgccagt accgtggtgt tactgcgcgc
    21061 acttgaagaa cggcaattaa ttgacagtca gcgtgggcaa atcgccatcg gttggttgat
    21121 tgtggaagac ctggtaatgg ttctgacgct ggtgttgctg cccgcagtgg caggaatgat
    21181 ggaacagggc gatgtgggct ttgccactct tgcagtcgat atggggatca ccatcggcaa
    21241 agtgatcgca tttatcgcca ttatgatgct ggtaggtcgc cgtctggtgc cgtggattat
    21301 ggcacgcagc gcggcaaccg gttctcgcga gctgtttacc ctgtcggtgc tggcgctggc
    21361 gttagggatt gcctttggtg cggtagagct gtttgatgtc tcctttgcac tcggtgcgtt
    21421 ctttgccggg atggtactga acgaatctga actgagtcac cgtgccgccc acgatacgct
    21481 gccattgcgc gacgcgtttg cggtgctgtt ttttgtctcc gtcgggatgt tgtttgatcc
    21541 gttaattctg attcagcaac cgctggcagt gctggcgacg ctggcgatta ttctgtttgg
    21601 taagtcgtta gccgcatttt tcctggtgcg actgtttggt cactcccaac gtacggcatt
    21661 aaccatcgcc gccagcctgg cgcagattgg tgagttcgcg tttatcctgg cgggactggg
    21721 aatggcattg aatttactgc cgcaggccgg acaaaacctg gtactggcag gggcgatcct
    21781 gtcgattatg ctcaacccgg tactgttcgc actactggag aaatatctgg cgaagaccga
    21841 aacgctggaa gagcagacgc tggaagaggc aatcgaagaa gagaaacaga tcccagtgga
    21901 tatttgcaac catgcgctat tggtgggtta cggtcgtgta ggcagcctgc tgggggagaa
    21961 attgctcgcc tctgatattc cgctggtggt gattgagacg tcacgaaccc gtgttgatga
    22021 gctgcgagag cgtggggtcc gcgctgtatt gggcaatgca gcgaacgaag aaattatgca
    22081 actggcgcat ctggaatgtg caaaatggct gatcctgacg atccccaacg gttatgaagc
    22141 gggtgagatt gtggcgtctg cccgcgcgaa aaacccggat attgagatta tcgcccgcgc
    22201 ccattatgac gatgaagtga cgtatatcac cgaacgtggc gcgaatcagg tagtgatggg
    22261 tgaacgtgaa attgcccgta ctatgctgga attgctggaa acgccaccgg caggtgaggt
    22321 ggtgacgggg taaaacaaga cgttattgcc ggatgcgatg ctagcgcatc ttatccggcc
    22381 tacggatgcc atcgtcgtcc ggtctggtcg ataacttaac gatcccagta agactcttcc
    22441 aggctgtctt cacgctccgg caagccgcgc gttaaacgag gtgaatgctg gttcagtacc
    22501 tgatagctca cacggttagc atatttacac acctgcgcta acgatgaata agttaaccag
    22561 gtgaatttat gtttgctgga gtttggtacg ttgctacgat ggtagctgtt ggcggtaatg
    22621 tcatgcagca acgctgccaa tgcgccatcc cccgctccat tagtgttcat gattttttcc
    22681 ggcccgccca tgtacggcgc aatgtgcgaa tatacacgca gcggattctg gcaatccttg
    22741 tggcgcatgg cgcggctaaa ctcatactgg ttgaattccg ctatagcgcc cggcagcagc
    22801 ggatgctggg ttttacgttt cgcttcgtct tcggtaaagc ccgccatata caagccgatt
    22861 ggcccggcgg tgcacagcac cagatctacc cagtccagcg ccttgtcaga tgccaacaac
    22921 ggatcgcttt ctccggtcaa cgcttcggct tcatcttcgt tcatcgcaag gatagagacg
    22981 tggtctttga ggaattgctg ccaccactgc ggattctcgg caatgacaaa cttagttccc
    23041 agcgtcagca ccaccggtac gttatatttc ttcgcgtact caatggcttt catggttgct
    23101 tccggcatgg gttcacccgg cttgcaacgc accagataag aggtgagaac cagagccgag
    23161 gctccggcaa tcacatcttc cggaatactt tcagcccgca gctggttcat gtggccaggg
    23221 ctgatagcaa aggtacgttc cccggactcg ccaatcagcg taaagcaacg accaatcggg
    23281 ccatccacgc cttgtagata gttaagatcg gtacggctgg aggtgttaca caggtaacga
    23341 taggcatagc tgccaatttc aatattgctg cacatgacgc ccagcagcac cgaacggtcg
    23401 tccgcgagca ccgagtagtt atgcatggtg ttaccaatgg tgccacccgc aaactgatgg
    23461 gtaatcaggt ttttctgttt tagttcctga taaagcgctt cggctacatc atcctcaatc
    23521 accagtgaat gcccggcgct taatccataa cgctcaatga attcatcatc cactttcgct
    23581 tcaatatcga ccagcgtctg gtcaatacct acgacccagg cggcgctggt ttcgttttct
    23641 ggctggaatt gctgaagcag cggatcgcgt gcatttacag ggaagtaatg tttggattta
    23701 cgtttaccgg gaaatttcat ggttgttttt acgggtggtt aactgagcgg cgaatgttaa
    23761 cacaatctga cgggagcgcg cgagacgcag ccagtttcaa tatcagcagg cgggatcgca
    23821 ttttcatggc agtggttcct tacaatgacg taatttgaaa ggagtttttg tatgaagccg
    23881 gaaaacaaac tacctgttct ggaccttatt tctgctgaaa tgaagaccgt tgtgaatact
    23941 cttcagccgg atttaccgtc ctggcccgca acgggaacga ttgctgagca acgacagtat
    24001 tacacgcttg agcgccgatt ctggaatgcg ggcgctccag aaatggcaac cagagcttac
    24061 atggttccaa caaaatatgg gcaggtggaa acacgtctct tttgtccgca gccagatagc
    24121 ccggcgacgc tattttattt gcatggaggc ggttttattc tcggcaatct cgatacccac
    24181 gatcgcatca tgcgcctgct ggcaagctac agccaatgta cggtgattgg tattgattac
    24241 cccctttcac ctgaagcgcg ttttccgcaa gcgatagagg aaattgtggc tgcttgttgt
    24301 tatttccacc agcaggcgga ggattatcaa atcaatatgt cccgcattgg ctttgccggt
    24361 gattccgcag gtgccatgct ggcgctcgcc agtgcgttgt ggttgcgtga taaacagatc
    24421 gattgcggta aaattgcggg tgttttgctg tggtatgggc tttacggatt acgggattcc
    24481 gtgactcgtc gtctgttggg cggtgtctgg gatggcttaa cgcaacagga tttgcagatg
    24541 tatgaagagg cgtatttaag caacgacgcg gaccgcgagt caccgtatta ctgtctgttt
    24601 aataatgatc tcactcgcga agttccgccc tgttttattg ccggggcgga gttcgatccg
    24661 ctgctggatg acagccgtct gctttaccag acgttagcgg cgcatcagca gccctgtgag
    24721 ttcaaactct acccaggtac gctgcatgcc tttttgcatt attcacgaat gatgaaaacc
    24781 gccgacgagg ctcttcgcga cggcgctcag ttctttaccg ctcagcttta gcgatacgcg
    24841 gcaacaagat tagccatcat ttcgatatgt tccggcgtgg cattaagcgc tggaatatat
    24901 tcatattttt tcccgccggc accgaggaag acctcacggt tttgctcggc aatctcttcc
    24961 agcgtctcca gacaatccgc agcaaagccc gggcacatca cctgtatatg acctacgcct
    25021 ttttctccga gcattttcag cgtttcgtcg gtataaggca tcagccaggg ttcccggcca
    25081 aagcgcgact gaaaggtcat catcactttt tccggtgcca tccccagtgc ggaagccagt
    25141 tcgcgagtcg ttgtgcggca acgttgcggg taatcatcgc cttcatctgc ataacgctgg
    25201 ggaatgccat gataagagag cagcagcaga tccggttcgc catgtttggc aaaagaagcg
    25261 cgtacgctgt tcgccagtgc attaatgtaa tcgtggttat cggcgtaatc acgaataaac
    25321 gatatccccg gaatgctacg tttgcgcgcc agaatgcgtg ccagttcatc ccataccgca
    25381 ccgaccgtag aacaggagaa ttgcggataa agcggcagca ccacaatatg atctacatgc
    25441 tctgccagga gttcatcgac ggcgctttcc agtgatggcg agccgtagct catgcccagc
    25501 gctacgggcg tatccggtaa acgttgtgcc aacgcctgct gttgctggcg gctgtaaacc
    25561 atcagcggcg agccgtcttc catccagacc gaggcgtaca gcttcgccac acgcggcgag
    25621 cgcagcggca aaatcacgcc gcgcagcaac ggccaccata acaaccgtga ggtatcaacc
    25681 acgcgtctgt cgcttaaaaa ttgtttcaga tagcgtttta ccgcttcagg tgtgggggca
    25741 tcgggcgtac ccaggtttgc cagcaggata ccggttttag tctgacgcat taccgcctct
    25801 tatcgattca acttgttgat aattgtagcg gaaaagtgag gaagaagaac taattgctgt
    25861 gagaggtcaa atcagaaaaa ggaatagcgc agattgaggt ttttgtaaaa ggccgaatga
    25921 atatccggcc tgagattgct gataagtttg cttgtgcggg cctgttcccc tcaccctaac
    25981 cctctccccg agggggcgag gggactgtcc gtgcgcgctt tcgaattagc cgaggatttt
    26041 ttccagatca gcgcgaactt cagcaaccgg cttggtgccg tcaactttcg cgtatttggt
    26101 gttacccgct tccgcttctt tggagtagta gccgatcagc ggtgcagtca tctgatggta
    26161 ttcaaccaga cgtttacgca cggtttcttc ctgatcgtct ttacgggtag tcagttcttc
    26221 accggtaacg tcgtctttgc cttctacttt cggcggattg aatttaacgt gataaacacg
    26281 accagacggc gcatgaacgc ggcggcctac gatacgatca acaatcagtt cgtccggtac
    26341 gtcgaattcc agaacgtaat caacattgat gcccgcttct ttcatcgcgt ctgcctgcgg
    26401 aatggtacgc gggaagccgt ccaacaggaa accattacgg cagtcttcct gagcaatgcg
    26461 ctctttaacc agcgcgatca ccagttcgtc ggtgaccagt ttgccagcat ccataatgtc
    26521 ttttgcttgt ttacccagct cggagccaga tttgaccgca gcacgcagca tatcgccagt
    26581 ggagatttgc ggaataccat atttctccat gatgaactga gcctgagtcc ctttccccgc
    26641 gcccggagcg ccaagcagaa tgatacgcat tgcgaaaatc cccttaaaat gtgtcgaatt
    26701 ttttgaaaaa gcgataaacg ataccaccaa caggcgattg cctcaagaaa ggcacctgag
    26761 gctgaaacgg ttaatggcga gataatttgc ggaaagtgca gatgatgaaa agaaaaatgc
    26821 cggatgacac aaaggtcatc cggcatgaca tcaggaaacc agcagctggt tcatacgacg
    26881 aataaacagg ttcggatctt ccagcgtgcc gcgttctgcc agcagcgcct gatccagcag
    26941 cagttctacc cactcgctga acttcgcttc atcttcagta tctgccgcac gtttcaccag
    27001 tacgtgatcc gggttcagtt cgaagatgta tttcacttct ggcactttct ggcccgccgc
    27061 agcgaacagt ttcgccatct gagtgctcat ttcgtccgcg tcggtcgaaa cgatcgctgg
    27121 cgtatcggtc agacggtgag tcagacggac atctttcacg cgctcgccga gcagggcttt
    27181 cacacggtcg atgaacggag tcagtgcttt ctccgcttct ttcgcgctct catcaacttc
    27241 gtcagccagt ttttccagcg actcgtcaac tttagacacc gactggaacg gtttaccgtc
    27301 gaactcagtc agatagttca tcatccactc atcgatgcgg tcggaaagca gcagaacttc
    27361 gatgcctttc ttacgcagca gttccaggtg tgggctgctc ttcgctgccg catagctgtc
    27421 tgcagtgatg tagtagattt tctcctgccc ttctttcatg cgggaaacgt agtcttccag
    27481 agacacggtc tgcgcagaag aatcggtata agtagaagca aaacgcagca gtttggcgat
    27541 cgcttcctgg ttagcgaaat cttccgccgg accttctttc agtaccaggc caaactgttg
    27601 ccagaaggtc tggtattttt ccgcgtcgtc tttcgccagt ttttccagca tttgcagcac
    27661 acgcttggtc agcgcattgc gcaggttacg cgttaccgtg ctgtcctgga ggatttcacg
    27721 ggaaacgttc agcggcagat cgctggagtc aatcagacca cgcacgaagc gcagatagtt
    27781 cggcatgaac tgttctgcgt cgtccatgat gaacacacgc tggacgtaca gtttcaggcc
    27841 gtgtttatga tcacggttcc acatatccca cggagcctgg gacgggatgt acagcaggct
    27901 ggtgtactcc tgcttacctt caacgcggtt gtggctccag gtcagcggat cattaaagtc
    27961 gtgggcgatg tgtttgtaga actctttgta ctcttcatcg gtgatttccg acttgttacg
    28021 agtccacagc gcctgcgctt tgttgatttt ctcccaggag ataacggttt cgccgtcttt
    28081 ctcttcgcgt ttttcgatct ctaccggcag cgcgatatgg tcggagtatt tgctgatgat
    28141 ggaacgcacg cgccagtcat cgaggaactc gtcttcgcct tcacgcagat gcagggtgat
    28201 ttcagtacca cgatcttctt tggtgatgtc ggcaacggta tattcacctt cgccagccga
    28261 ttcccagaag acgccgtttt ctggtttttc gcctgccgcg cgagtacgca cggtcacttt
    28321 gtcggccacg ataaacgcag agtagaaacc aacaccaaac tgaccgatca gctggctgtc
    28381 tttcgcctgg tcagaaccca gggattcgag gaatgatttg gtaccggatt tagcgatagt
    28441 ccccagatgg tcaatcactt cgtcgcgggt catccccacg ccgttatcgg agatggtcag
    28501 cgtgcgcttg tctttatcga aagagacacg aacgcgcagt tcgccatcac cttcgtacag
    28561 gtccgggtta gatagcgcac ggaaacgcag cttgtccgcc gcatcggagg cgttagagat
    28621 aagctcacgc aggaagattt ctttattgga atagagagaa tggatcatca ggtgcagaag
    28681 ctgtttcact tctgactgaa aaccacgagt ttcttgtcct ttcatgtagg tctacctcaa
    28741 taatgccatt ttaaggttaa aaacaggatg agggagagat ggggacaagg gagaataatt
    28801 tcaagcgaga gcaggtgatc ctgctctcgt ttgcttaaaa acgaatctta tgacgcccgg
    28861 caagggagtg tgacagcgtg gtgccatcga ccatttccag ttcgccgcca accggtacgc
    28921 catgagcgat tcggctggct tccacgtcat attgcgcgca aagctcggca atgtagttag
    28981 cggtagcttc accttcaacc gtgggattgg tggcgaggat cacttcagtg attttttcct
    29041 ctgccagacg ctgttccaga cgatcaaggc cgatatcatc cggaccgatg ccgtccagcg
    29101 gtgacaggtg ccccatcaac acaaaataac ggcctgaaaa ctgccccgtc tgctcaatgg
    29161 cgtagatgtc cgccggactc tccaccacac agatttgacc gttttcctga cgacgcggat
    29221 tcgaacagat gttacagact tcctgttcgg tgaaagtgcg gcaatcggcg cagtggccga
    29281 tttccgacat cgcccgggtg agcgcctgcg ccagacgcat cccgccgcta cgatcgcgct
    29341 gaagcagcgt gaacgccata cgctgcgccg acttcgggcc aacgcccggc agacagcgca
    29401 gtgcttccat aagctgtgtt aacagcgggc tggtttgcat cagaacggca tcttaaagcc
    29461 aggcggcagc tgcattccgg aggatacaga ggccattttt tctttctgcg tttcttcaat
    29521 acgacgtgct gcgtcgttga atgctgcagc caccaggtct tccagcatct ctttgtcgtc
    29581 ttccagcagg ctcgggtcga tctctacgcg acggcagttg tgtgcaccgt tgatggtcac
    29641 ttttaccaga cctgcgccag attcgccggt gacttccagc tgcgcgatct cttcctgcat
    29701 tttctgcatt ttttcttgca tctgctgggc ttgcttcatc aggttaccca gaccgccttt
    29761 accaaacata ggtttctctc tcaatcacgt taaggatgac gaacgtaagc tgtgcttacg
    29821 atcaaatggg gcggatactt tcttcatcca gctccgcatc gaagaaccga cgcagggtct
    29881 gaatattatt atccgcaata atggactcgc gcgcctgcgc aagtttttct tcgtatatcg
    29941 cctgacgcca ctccagcggc gtacgcaccg cgggattatc atcttcaacg atagtcagtt
    30001 caaccgttga accttttaac gtgctcaacg cttcagccag tttttgctgt gcaccgcggt
    30061 tgttcaaatg ccgctgagag gagcgcaaat gcagacatac tgcgttgtcg ctctcctctt
    30121 tccaggcatt taaggccacc tgttcgacca gttttggtag cgaaagttga ctcacctgtg
    30181 ccgcccacgc gtcgcgctca atggcttctg ccgccagctt cgccgccagt tccggcgttt
    30241 tttcatgttc cagcgctttt ttcagcgcct tcggcgtggc gaccacttct ttttgctgca
    30301 tcactggagt ggtagccttc cagcgatacg cttctttttt ggctggcgct ttttccagcg
    30361 ccgatggcac cggacgcgcc tgaacgcgat cggtgaccga agccagtctt tccagcgcag
    30421 cgttattcac cggccgcgcg cgggtagcgg ctgccggttc actctttttt gctttggttg
    30481 ctccctgcac gcgctgcaac tgctggcgcg ccgccagcac ctggctggtg gtttccggga
    30541 gcggtacagt cggtgcctgc tgcggcgctg attgcggttg cggcggcacc tgggttggcg
    30601 tcattactgc cgttggcgcg acgggcgcaa aggactggcg tggcacttct ggctcaggca
    30661 gaggcatacg tggatggaac gccagcgcgc gcagcagcgt catctcaacg cccatgcggc
    30721 ggtccggcgc atacggtaat tctttgcgac caatcaacag cgtctgatag taaagctgaa
    30781 tatccgtcgg cggtatggtg cgcgccagtt cacgcatccg cagctcaatg gcggccatgt
    30841 cgttgccaag tgcagcaggc gaaagttgta ccatcgcaat acgatgcaac aggccgagca
    30901 tttccaccag caacgcttcc cactcgatac cacgggcagc ggcttcatta atcagcgcca
    30961 ttacgcgctc gccgttggcc tcgaccatcg cttcaaccag cgacagcgcc tgatcgtcgt
    31021 caagcgtacc cagcatcgca ctgactgcct gggttgaaac ctggccgtca ccgctggcaa
    31081 tcgcctggtc ggtcagactt aaggcatctc gcaggctgcc ttcagcggcg cgcgccagca
    31141 attgcaacgc ccgcggctcg tgagcgatat gttcttcgtt gaggatgtgc tcaagttgat
    31201 ggcgaatttg ctcgacatcc agcgccttga gatgaaattg caaacagcgc gacaaaatcg
    31261 tcaccggcaa tttctgtgga tcggtcgtcg ccagcagaaa cttaacgtgc tccggcggct
    31321 cttcaagggt ttttaacagt gcgttaaagc tgtggcgcga cagcatatgc acttcgtcga
    31381 tcagataaac tttgaaacga ccacgcgccg gagcgtactg gacgttatcc agcaggtcgc
    31441 gggtatcttc aactttggtg cgcgaggcgg cgtcgatttc aatcagatcg acaaagcgcc
    31501 cctgctcgat ttcacgacag ttatcgcaca cgccgcacgg cgtcgcggta atgccggttt
    31561 cgcagtttag ccccttcgcc agcagtcggg cgatagaggt ttttccgacg ccacgggtac
    31621 cggaaaaaag ataagcatga tgaatacgcc ctaacgacaa gccgttcgcc agtgcggtca
    31681 gcacatgttc ctggccgacg acgtcagcaa aggtttgtgg gcgccatttt cgggctaaga
    31741 cctgataact cattggcagg ctctgaaacg ctggaaggtg gattcacgaa ggggtaatgc
    31801 taacacagcc ccgtcagaac ggcgaggctg aatgcacatc ttgtgctgtc cgtagcgtgg
    31861 gcagcacaag actggcgata attaatggcc cgggaacggg acaaggctgt agctggtaat
    31921 gccctgtttt tcgagacgct gttcgccgcc gagatcgaac aggttgataa tgaacgcagc
    31981 gtcagccact tcaccaccca gacgacggat cagtttaacg gtcgcttcga tagtgccgcc
    32041 ggttgccagc agatcgtcca ccaccagaac tttgtcgccc ggtttgatgg catcaacgtg
    32101 gatctccagc tgatcggtgc cgtattccag gtcgtaagtt tcactgatgg tttcacgcgg
    32161 cagtttgccc ggtttacgga ccggtacaaa gccaacgccc agacccagag ctaccggagc
    32221 gccaaacaag aagccacgcg cttcggtgcc gacaactttg gtaatgcccg catttttgta
    32281 acgctcaacc agcaagtcga tgctgagagc gtaagctttc gggtcttcca gtaagctggt
    32341 gacatcgcgg aaaagaatgc cgggttttgg gtagtcctga atgcttttga tgctattttt
    32401 gagatactca agctgctgtg cagtcgcggt cataagtgta tgcctgcttg ttacggtggt
    32461 actcacggcg cgttttaaac gtatcaaaag taacagttgt ttaacctttg accgcctgcg
    32521 cctgtgctcg aaaacgccag aatgtactgg ctgtacgcaa taattgcaac tgcgattgtt
    32581 gtgcttcagt gcttttcttg cttttcatca atcaccggaa ttcgccacat atagaaaagc
    32641 aaacaggcga gaattaccag caacatgatg cgcacccatg gcatctggac gaaccacaga
    32701 gaaatggcaa acgtgagcaa aataagcaaa atcgcccgcg gtttgacgcc gcgcggcatc
    32761 gcatgatgtt tctgccagaa acgtagatag ctgccaaacc atgagcggta cagcaaccag
    32821 gcgtgaaagc gcggggaaga acgggcaaag caccaggccg ccagcaggat aaacggcgtc
    32881 gtcggtaata ccggtaatac cacgcccagc gtacccagca ctaccgccag ccagccaatg
    32941 atgattaaaa tgattcgttg catattgagt gttgtcagct tacagagtgg ctactttagc
    33001 ataacaatta tcattttcat tgaggtctta tcgtgaaaac cgccctgctg ctggaaaaac
    33061 tggaaggaca gctcgctacg ctgcgtcagc gttgtgcccc ggtagcgcaa tttgccacgc
    33121 taagtgcccg ttttgacaga cacctttttc aaacccgtgc gacaacactg caggcttgtc
    33181 tcgatgaggc gggcgataat ctggctgcgc ttcgtcatgc agttgagcag caacagctgc
    33241 cgcaagtggc ctggctggcg gaacatctgg cggcacaact ggaagccatc gcgcgtgaag
    33301 cctccgcctg gtcattgcgc gagtgggaca gtgcgccacc gaaaattgcc cgctggcagc
    33361 gtaaacgtat tcagcatcag gattttgagc ggcggctacg tgagatggtt gccgaacgca
    33421 gagcccgcct ggcgcgggtg accgatctcg tggaacagca aacgctgcat cgtgaagtgg
    33481 aagcctatga agcgcgcctg gcacgctgcc gccatgcgct ggaaaaaatc gaaaacaggc
    33541 tagcgcgctt aacccgctag caatggagag agtatgtcac tggaaaatgc ccctgacgat
    33601 gtcaaactgg ctgtcgattt gattgtgctg ctggaagaaa atcagatccc agcccgcacc
    33661 gtgctgcgcg cgctggatat tgtaaaacgc gattatgaaa agaaattaac gcgcgatgat
    33721 gaggcggaaa agtgagaaat aaatggggcc gctaaatgtc ccctaagatt gtgggcaaac
    33781 gcaaaactgc ctgatgcgct acgcttatca ggcctacacc gctcctgcaa tacattgaat
    33841 tcttgcggtt tcgtaggccg gataaggcgg tcacgccgca tccggcatga acaacgcgca
    33901 ctttgtcagt aatctcgggg caactaaatg tcgccacctt tggttattac cctaccgctg
    33961 gcgtcgggtc atcgcctttg tagtcgcgtt tgacttccgt cacctcatcg cccttctcgt
    34021 tgtgcagatg cacttcaagc tggttaaagg caatgttgat gtcgttttca cggcacagct
    34081 gatcgatagt acggttcagc tcatcgacag tacgactacg gtcacgcagt tcacgcacat
    34141 acagacgcag ctcatgatcc aacgtgctgg caccaaacgc cgtaaagaag acttccggca
    34201 ttggttcgtg catcaccctt gggtgctcag tcgccgcctt cagtaacact ttacgcactt
    34261 tttccagatc ggagccatag gccacgccga gacggatcac cagacgcgta gtagtgtcag
    34321 tcaacgacca gttgatcaga cgctcggtaa caaacgcttt gttcgggatg atcacttctt
    34381 tgcgatcgaa atcggtaatc gttgtcgcac gaatacggat cttacttacc gtccccgaga
    34441 agctaccaat ggttaccgta tcaccaatac gcaccggacg ttcgaacagg atgatcaaac
    34501 cagagacaaa gttaccgaag atctcctgta agccaaaacc aagacctacg gataatgctg
    34561 ccgccagcca ctggagttta tcccacgaga cgcccagcga tccgaacacc gtcatcgcac
    34621 caacggcaat aatgatgtag ttaaggatgg tagtaatggc atacgacgcg ccctgacgca
    34681 tattcagtcg cgagagcacc agcacttcca gtaaaccagg caggttgcga atcaacgccc
    34741 aggccaccat tgaggcgata atcgcaaaca acagactgcc catggtgacg tttttcacca
    34801 ccgcagcgcc agcttcagtg ccgttgtaat gccagagcgt gatgctgtcg agatagctga
    34861 acacggtgat caaatcggac caaattgccc agaacatgac accgaacagc gcaaacatca
    34921 gcaacatggt aatacgcagc gtctgctggt taacctgttc cagtgcaatg gtgggttctt
    34981 ccggcggttc ggcaccttct gcgccctctt tcaccagatt ctgccgacgc gccagcgcac
    35041 gacgccaggc gatacgccgc gccgctacgc ttaagccgcg caataccgtc tggtagagca
    35101 agttccagat gatcaccaga taaacggttt caatccaacg cccggagagg cgcagcgtgg
    35161 tgtagaaata acccgtggca gtcagcacca tcaatgcaat cgggataatc gacagcacgg
    35221 taatggtgac cagtcgcatg gtgtgcgact ctttatcacg ccagctttcg cggcacattg
    35281 gccataccag gaaggcaatc agcagcaggt tgaagaaaat catcgcctgc cccagcacat
    35341 catccatcag atgcagcggg gaaagttctg ccaccacaga ccagaaatgg ataggcagca
    35401 acgcgagact gatgcggaca atttgccgac gccagtggct ggtctgctgc tccggcatgc
    35461 cgaagtgacg cacggcaacg ccatttttct ccagtacctt ccagcacagg ccaaacacca
    35521 gccagaaaat cgccagtttt ttactgaacg accatagcag ttcgctgatg ttgagctgca
    35581 tggtcaacag aatcaggcca accgcgagaa taatcaggca caccggcagc gcgcggatca
    35641 ggtcgataag gatcgctttt ggtgtgttga gctggctatc gttacgcagg gaacccacag
    35701 cggaagccag tttctgttga tacgctttca gccagcccag acgccagtgg atcagcccgg
    35761 caatcaacag cagcggcaaa ccggcgagga aagcgataaa aacggcaggc caggcttttt
    35821 cccagttcac cgtgattttc atcgacttaa attcatcttt caggctttgc gggaacgctt
    35881 tgatccagtc ccagtccatt ggacggttac tgttcaccca aaagatttgc tgagtcagga
    35941 tggatttcag gtttttcgat acgctcatta actgctgctg gttgatttgc aggttaatgg
    36001 ccatcatcag ctggttaccc aactgtttgt tgagttgatc cagcaattcg cgacgcatat
    36061 caaccacttg caataacgca tcgtgaactt cgctgttgac ttcgttggtg tgaccttctt
    36121 ccagtttgtt gacgaatgca tcgctctgga agagtgcatc acgctgctgg ttaacttcaa
    36181 actgttcgag acgcaaatcc gcaatgcggt tggtcatgtt ttccagttca tccgccgagg
    36241 gcagcgtttg ttgttgctgg taaaggatac gagacaacag caggctgccc ttcaggacgg
    36301 caatctgctc tttaatattg cgttccgatt gcagcgcccg ctccagccag tttttgactt
    36361 taatgttttg ctgcatcaac tgattaccgt tttcagtcgc ggtaatcaga cgctgactta
    36421 actgctggtt aatttccagt tcctgtttca ccagcggatt agcctgaata cgcgcggctt
    36481 catccgggga gacggcttcc tgcgccgttt tttcagttaa agtcaggcgc ttgctgttta
    36541 ccgcttcttg caacagctgc aactggtgct ccagacgggc gctgttcgcc gtcacgtaat
    36601 cacgttgctt ttgcaaggta tcctgcaaga cggtgttccc ttccaggctt ttacgctgct
    36661 ggtcaatctc cgcattcagc aacgcctgct gagcctgcat taacactttc tggctgggac
    36721 gtaaggcagt ctcgccgaca tcagtcccat ccagacgact gcgaatttgt tgcagctgct
    36781 gcgaagcatt atacatcgca ttttgcacgc gttcgggctg cgtctgtaac gaaaccagct
    36841 ggctgttata agacgccaga tcgttttgtg cgttttgcaa atcgtccagc gcctgggcaa
    36901 cgcgagtttc cagctggcgc aacgacagcg tactcagaat tttgcgcgtt tcttcgtcat
    36961 tatcgacatc gctaagtgca gttaacgccg cggtcgcctg gcgcattttt tccggcgctt
    37021 cagcaacctt ttgccgtagc tgaactgtct cttcttttat gcgatcgatt ttatcgaggg
    37081 tggctaatgt atctgtcaga tcctgctgca ccagtttgtc ctgagcagaa agatcttttt
    37141 gtttatttag tgagtcaagt tgcgcctgca ggtccgcttt tgtcggcaga tcaccattcg
    37201 atgacgcccg cgcaaacgcc gtgttctggc ataacaagac aaaaacaaaa gcaataaatg
    37261 ctgaaaaaac aaaatgccgt gatcgtttgt aatactggaa catagtcatg atgaatgaag
    37321 gtttctgaac ctgaagaacg acctgaaaaa gtcaaaccgc aagaatatca cgacgcagtg
    37381 aaccagaata gcaacgacga aaatgtccag gaaaaatcct ggagtcagat tcagggttat
    37441 tcgttagtgg caggattacg aagcgtgggg cacaggagat acatctccag taagatggcg
    37501 acgtaatcgc gggcttcttt tttaagatca aaagattgcg gggcaaagag ccagttttcc
    37561 atcaggccgg aaatatagcc gcgcataata attgctgccc gacgcgtcat taaatccgca
    37621 ggcaacattt tcgcttcaat acaatgtttt aacgtttgtt ctatacggtc ataactttcc
    37681 agacagagat tacgttgtgc ctgttgcaca acagccattt ctccgacaaa ttcgcatttg
    37741 tggaatataa tctccatcaa taatcgacgt cgttcttctg tcaccgtgga ttcaagaaca
    37801 tgaattaata tctctcttaa tactgagagt ggatcgccag ggaattttgc ctgatactca
    37861 agctctagtt caccaatatt ggattctgac agttcccaga tctcactgaa caaatccgac
    37921 ttgtctttaa aatgccagta gattgcaccg cgcgtaacgc cagctgcttt tgcaatctcg
    37981 cccagcgagg tggatgatac cccctgctgt gagaaaagac gtagagccac atcgaggatg
    38041 tgttggcgcg tttcttgcgc ttcttgtttg gtttttcgtg ccatatgttc gtgaatttac
    38101 aggcgttaga tttacataca tttgtgaatg tatgtaccat agcacgacga taatataaac
    38161 gcagcaatgg gtttattaac ttttgaccat tgaccaattt gaaatcggac actcgaggtt
    38221 tacatatgaa caaaaacaga gggtttacgc ctctggcggt cgttctgatg ctctcaggca
    38281 gcttagccct aacaggatgt gacgacaaac aggcccaaca aggtggccag cagatgcccg
    38341 ccgttggcgt agtaacagtc aaaactgaac ctctgcagat cacaaccgag cttccgggtc
    38401 gcaccagtgc ctaccggatc gcagaagttc gtcctcaagt tagcgggatt atcctgaagc
    38461 gtaatttcaa agaaggtagc gacatcgaag caggtgtctc tctctatcag attgatcctg
    38521 cgacctatca ggcgacatac gacagtgcga aaggtgatct ggcgaaagcc caggctgcag
    38581 ccaatatcgc gcaattgacg gtgaatcgtt atcagaaact gctcggtact cagtacatca
    38641 gtaagcaaga gtacgatcag gctctggctg atgcgcaaca ggcgaatgct gcggtaactg
    38701 cggcgaaagc tgccgttgaa actgcgcgga tcaatctggc ttacaccaaa gtcacctctc
    38761 cgattagcgg tcgcattggt aagtcgaacg tgacggaagg cgcattggta cagaacggtc
    38821 aggcgactgc gctggcaacc gtgcagcaac ttgatccgat ctacgttgat gtgacccagt
    38881 ccagcaacga cttcctgcgc ctgaaacagg aactggcgaa tggcacgctg aaacaagaga
    38941 acggcaaagc caaagtgtcg ctgatcacca gtgacggcat taagttcccg caggacggta
    39001 cgctggaatt ctctgacgtt accgttgatc agaccactgg gtctatcacc ctacgcgcta
    39061 tcttcccgaa cccggatcac actctgctgc cgggtatgtt cgtgcgcgca cgtctggaag
    39121 aagggcttaa tccaaacgct attttagtcc cgcaacaggg cgtaacccgt acgccgcgtg
    39181 gtgatgccac cgtactggta gttggcgcgg atgacaaagt ggaaacccgt ccgatcgttg
    39241 caagccaggc tattggcgat aagtggctgg tgacagaagg tctgaaagca ggcgatcgcg
    39301 tagtaataag tgggctgcag aaagtgcgtc ctggtgtcca ggtaaaagca caagaagtta
    39361 ccgctgataa taaccagcaa gccgcaagcg gtgctcagcc tgaacagtcc aagtcttaac
    39421 ttaaacagga gccgttaaga catgcctaat ttctttatcg atcgcccgat ttttgcgtgg
    39481 gtgatcgcca ttatcatcat gttggcaggg gggctggcga tcctcaaact gccggtggcg
    39541 caatatccta cgattgcacc gccggcagta acgatctccg cctcctaccc cggcgctgat
    39601 gcgaaaacag tgcaggacac ggtgacacag gttatcgaac agaatatgaa cggtatcgat
    39661 aacctgatgt acatgtcctc taacagtgac tccacgggta ccgtgcagat caccctgacc
    39721 tttgagtctg gtactgatgc ggatatcgcg caggttcagg tgcagaacaa actgcagctg
    39781 gcgatgccgt tgctgccgca agaagttcag cagcaagggg tgagcgttga gaaatcatcc
    39841 agcagcttcc tgatggttgt cggcgttatc aacaccgatg gcaccatgac gcaggaggat
    39901 atctctgact acgtagcggc gaatatgaaa gatgccatca gccgtacgtc gggcgtgggt
    39961 gatgttcagt tgttcggttc acagtacgcg atgcgtatct ggatgaaccc gaatgagctg
    40021 aacaaattcc agctaacgcc ggttgatgtc attaccgcca tcaaagcgca gaacgcccag
    40081 gttgcggcgg gtcagctcgg tggtacgccg ccggtgaaag gccaacagct taacgcctct
    40141 attattgctc agacgcgtct gacctctact gaagagttcg gcaaaatcct gctgaaagtg
    40201 aatcaagatg gttcccgcgt actgctgcgt gatgtggcga agattgagct gggtggtgag
    40261 aactacgaca tcatcgcaga gtttaacggc caaccggctt ccggtctggg gatcaagctg
    40321 gcgaccggtg caaacgcgct ggataccgct gcggcaatcc gtgctgaact ggcgaagatg
    40381 gaaccgttct tcccgtcggg tctgaaaatt gtttacccgt acgacaccac gccgttcgtg
    40441 aaaatctcta ttcacgaagt ggttaaaacg ctggtcgaag cgatcatcct cgtgttcctg
    40501 gttatgtatc tgttcctgca gaacttccgc gcgacgttga ttccgaccat tgccgtaccg
    40561 gtggtattgc tcgggacctt tgccgtcctt gccgcctttg gcttctcgat aaacacgcta
    40621 acaatgttcg ggatggtgct cgctatcggc ttgttggtgg atgacgctat cgttgtggta
    40681 gaaaacgtgg agcgtgttat ggcggaagaa ggtttgccgc caaaagaagc tacccgtaag
    40741 tcgatggggc agattcaggg cgctctggtc ggtatcgcga tggtactgtc ggcggtattc
    40801 gtaccgatgg ccttctttgg cggttctact ggtgctatct atcgtcagtt ctctattacc
    40861 attgtttcag caatggcgct gtcggtactg gtagcgttga tcctgactcc ggctctttgt
    40921 gccaccatgc tgaaaccgat tgccaaaggt gatcacgggg aaggtaaaaa aggcttcttc
    40981 ggctggttta accgcatgtt cgagaagagc acgcaccact acaccgacag cgtaggcggt
    41041 attctgcgca gtacggggcg ttacctggtg ctgtatctga tcatcgtggt cggcatggcc
    41101 tatctgttcg tgcgtctgcc aagctccttc ttgccagatg aggaccaggg cgtatttatg
    41161 accatggttc agctgccagc aggtgcaacg caggaacgta cgcagaaagt gctcaatgag
    41221 gtaacgcatt actatctgac caaagaaaag aacaacgttg agtcggtgtt cgccgttaac
    41281 ggcttcggct ttgcgggacg tggtcagaat accggtattg cgttcgtttc cttgaaggac
    41341 tgggccgatc gtccgggcga agaaaacaaa gttgaagcga ttaccatgcg tgcaacacgc
    41401 gctttctcgc aaatcaaaga tgcgatggtt ttcgccttta acctgcccgc aatcgtggaa
    41461 ctgggtactg caaccggctt tgactttgag ctgattgacc aggctggcct tggtcacgaa
    41521 aaactgactc aggcgcgtaa ccagctgctt gcagaagcag cgaagcaccc tgatatgctg
    41581 accagcgtac gtccaaacgg tctggaagat accccgcagt ttaagattga tatcgaccag
    41641 gaaaaagcgc aggcgctggg tgtttctatc aacgacatta acaccactct gggcgctgca
    41701 tggggcggta gctatgtgaa cgactttatc gaccgcggtc gtgtgaagaa agtttacgtc
    41761 atgtcagaag cgaaataccg tatgctgccg gatgatatcg gcgactggta tgttcgtgct
    41821 gctgatggtc agatggtgcc gttctcggca ttctcctctt ctcgttggga gtacggttcg
    41881 ccgcgtctgg aacgttacaa tggcctgcca tccatggaaa tcttaggcca ggcggcaccg
    41941 ggtaaaagta ccggtgaagc aatggagcta atggaacaac tggcgagcaa actgcctacc
    42001 ggtgttggct atgactggac ggggatgtcc tatcaggaac gcctctccgg caaccaggca
    42061 ccttcactgt acgcgatttc gttgattgtc gtattcctgt gtctggcggc gctgtacgag
    42121 agctggtcga ttccgttctc cgttatgctg gtcgttccgc tgggggttat cggtgcgttg
    42181 ctggctgcca ccttccgtgg cctgaccaat gacgtttact tccaggtagg cctgctcaca
    42241 accattgggt tgtcggcgaa gaacgcgata cttatcgtcg aattcgccaa agacttgatg
    42301 gataaagaag gtaaaggtct gattgaagcg acgcttgatg cagtgcggat gcgtttacgt
    42361 ccgatcctga tgacctcgct ggcgtttatc ctcggcgtta tgccgctggt tatcagtact
    42421 ggtgctggtt ccggcgcgca gaacgcagta ggtaccggtg taatgggcgg gatggtgacc
    42481 gcaacggtac tggcaatctt cttcgttccg gtattctttg ttgtggttcg ccgccgcttt
    42541 agccgcaaga atgaagatat cgagcacagc catactgtcg atcatcattg atacaacgtg
    42601 taatcactaa ggccgcgcaa gcggcctttt ttatgcataa cctacgaaca ttaaggagta
    42661 attgaaccac caactcagga tctcatacga aaaccagtat taaccacgga taaaattcat
    42721 a
//
LOCUS       DAZFFI010000028        42652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFFI010000028 DAZFFI010000000
VERSION     DAZFFI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.028"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..434)
                     /locus_tag="ACHJ12_001855"
     CDS             complement(60..434)
                     /locus_tag="ACHJ12_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989650.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            977..1110
                     /locus_tag="ACHJ12_001856"
     ncRNA           977..1110
                     /ncRNA_class="other"
                     /locus_tag="ACHJ12_001856"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            1854..3461
                     /gene="dppA"
                     /locus_tag="ACHJ12_001857"
     CDS             1854..3461
                     /gene="dppA"
                     /locus_tag="ACHJ12_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4989651.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            3612..4631
                     /gene="dppB"
                     /locus_tag="ACHJ12_001858"
     CDS             3612..4631
                     /gene="dppB"
                     /locus_tag="ACHJ12_001858"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4989652.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            4641..5543
                     /gene="dppC"
                     /locus_tag="ACHJ12_001859"
     CDS             4641..5543
                     /gene="dppC"
                     /locus_tag="ACHJ12_001859"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4989653.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            5554..6537
                     /gene="dppD"
                     /locus_tag="ACHJ12_001860"
     CDS             5554..6537
                     /gene="dppD"
                     /locus_tag="ACHJ12_001860"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4989654.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            6534..7538
                     /gene="dppF"
                     /locus_tag="ACHJ12_001861"
     CDS             6534..7538
                     /gene="dppF"
                     /locus_tag="ACHJ12_001861"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4989655.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(7568..8839)
                     /locus_tag="ACHJ12_001862"
     CDS             complement(7568..8839)
                     /locus_tag="ACHJ12_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4989656.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            9315..9422
                     /locus_tag="ACHJ12_001863"
     CDS             9315..9422
                     /locus_tag="ACHJ12_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989657.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            9798..9905
                     /locus_tag="ACHJ12_001864"
     CDS             9798..9905
                     /locus_tag="ACHJ12_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989658.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMSVNWLNKRK"
     gene            10281..10388
                     /locus_tag="ACHJ12_001865"
     CDS             10281..10388
                     /locus_tag="ACHJ12_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989659.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(10475..12154)
                     /gene="bcsG"
                     /locus_tag="ACHJ12_001866"
     CDS             complement(10475..12154)
                     /gene="bcsG"
                     /locus_tag="ACHJ12_001866"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4989660.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12151..12342)
                     /gene="bcsF"
                     /locus_tag="ACHJ12_001867"
     CDS             complement(12151..12342)
                     /gene="bcsF"
                     /locus_tag="ACHJ12_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4989661.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(12339..13910)
                     /gene="bcsE"
                     /locus_tag="ACHJ12_001868"
     CDS             complement(12339..13910)
                     /gene="bcsE"
                     /locus_tag="ACHJ12_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4989662.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14183..14371
                     /gene="bcsR"
                     /locus_tag="ACHJ12_001869"
     CDS             14183..14371
                     /gene="bcsR"
                     /locus_tag="ACHJ12_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4989663.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            14383..15135
                     /gene="bcsQ"
                     /locus_tag="ACHJ12_001870"
     CDS             14383..15135
                     /gene="bcsQ"
                     /locus_tag="ACHJ12_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO4989664.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15132..17750
                     /gene="bcsA"
                     /locus_tag="ACHJ12_001871"
     CDS             15132..17750
                     /gene="bcsA"
                     /locus_tag="ACHJ12_001871"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4989665.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            17761..20100
                     /gene="bcsB"
                     /locus_tag="ACHJ12_001872"
     CDS             17761..20100
                     /gene="bcsB"
                     /locus_tag="ACHJ12_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4989666.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFIKDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            20107..21213
                     /gene="bcsZ"
                     /locus_tag="ACHJ12_001873"
     CDS             20107..21213
                     /gene="bcsZ"
                     /locus_tag="ACHJ12_001873"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4989667.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21195..24668
                     /gene="bcsC"
                     /locus_tag="ACHJ12_001874"
     CDS             21195..24668
                     /gene="bcsC"
                     /locus_tag="ACHJ12_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO4989668.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLKLIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            24750..26738
                     /gene="hmsP"
                     /locus_tag="ACHJ12_001875"
     CDS             24750..26738
                     /gene="hmsP"
                     /locus_tag="ACHJ12_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4989669.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            26921..28207
                     /gene="dctA"
                     /locus_tag="ACHJ12_001876"
     CDS             26921..28207
                     /gene="dctA"
                     /locus_tag="ACHJ12_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4989670.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            28428..29924
                     /locus_tag="ACHJ12_001877"
     CDS             28428..29924
                     /locus_tag="ACHJ12_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4989671.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30020..30949)
                     /gene="kdgK"
                     /locus_tag="ACHJ12_001878"
     CDS             complement(30020..30949)
                     /gene="kdgK"
                     /locus_tag="ACHJ12_001878"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4989672.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31181..31948
                     /gene="pdeH"
                     /locus_tag="ACHJ12_001879"
     CDS             31181..31948
                     /gene="pdeH"
                     /locus_tag="ACHJ12_001879"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4989673.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32018..34078
                     /locus_tag="ACHJ12_001880"
     CDS             32018..34078
                     /locus_tag="ACHJ12_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4989674.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(34312..35634)
                     /locus_tag="ACHJ12_001881"
     CDS             complement(34312..35634)
                     /locus_tag="ACHJ12_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989675.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(36045..37058)
                     /gene="yhjD"
                     /locus_tag="ACHJ12_001882"
     CDS             complement(36045..37058)
                     /gene="yhjD"
                     /locus_tag="ACHJ12_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4989676.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(37107..38006)
                     /locus_tag="ACHJ12_001883"
     CDS             complement(37107..38006)
                     /locus_tag="ACHJ12_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4989677.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            38526..39128
                     /locus_tag="ACHJ12_001884"
     CDS             38526..39128
                     /locus_tag="ACHJ12_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4989678.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39179..40828)
                     /locus_tag="ACHJ12_001885"
     CDS             complement(39179..40828)
                     /locus_tag="ACHJ12_001885"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4989679.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            41233..42630
                     /gene="ccp"
                     /locus_tag="ACHJ12_001886"
     CDS             41233..42630
                     /gene="ccp"
                     /locus_tag="ACHJ12_001886"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO4989680.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        10256 a        10736 c        11682 g         9978 t
ORIGIN      
        1 cggaggaccg gatgggaatc gggagacctg gacaaaaagt gcaggcctcc atgacacggt
       61 caggacagtt tgtgtttaac cagaatattt agcgcctgta tcggggcttc gtggagttcc
      121 cgcagacatt cagggtcagt tacatgttcc tggtatcttg ttttctcaag taacaagcgg
      181 tgcatgtgca gcatacgggc aagagccgcg cacacctcct caaatgcggt atcccgctta
      241 ccgtcactgt cagagccaca aaacagcggc acaacctcca cgccgtcgct gtgggccttt
      301 agccaggcat caaccacggt taaatcatcg gtgaagaacc acgcactgtt agcgctgaat
      361 cgcattttcc aggcttccgg tactggttgc ggggagtatg aatatgctgg tggtatcttg
      421 tcgtgagtat ccataggcta tcgttccgtc attctgttat tcaccacggc gctaaaaacg
      481 ccatgaagga ccagaatacc gattgcctcg tttgctgtat gaggtgctta ccacacattg
      541 ggtacacaga tgaatcaggt ggaagtgtga ttaccggaaa aaaaaacctt tcaggttgac
      601 gaaaaaaaaa accgcagtgt gataacacta cggcacgcca ccaaagaaac ataacaagga
      661 atcaacaatg agtaacacct gtaagccttc ggggtgattg taacacatga gcaatctttt
      721 actggaaact gtttttcact ggatcacgta tcgttatggg gattcccata tcggagagca
      781 tccaccagct tcatagggga taaccaggaa aatgccggtt cattgttgta cattttttaa
      841 gcaaatcatt ttagctatac gtttggatat acgattttgg attgtggtta tcaatattat
      901 tatttttcat ttggttacaa gctatgtttg aatactctat caaattcgaa aagcctgctc
      961 agcgagcagg cttttttgca tttgaagccc atgaggatga gaacctccgg gggcggggag
     1021 gttcgactcg agcgaagcga gagaacgttg cgccagcaac ggcccgcagg gcgagccacg
     1081 aagtggcgag taatcctcta tcaccgacca aatccgaaaa gcctgctcaa cgagcaggct
     1141 tttttgcatc tgcagcccat gaggatgaga acttccgggg gcagggaggt tcgactcgac
     1201 gtaggcctga taagacgcgc cagcgtcgca tcaggcaacg gcaccgtact tattgtaaga
     1261 tgcggcgtga acaccttatt cgacctacac accacacatt ccctgccaaa tttgcgcccc
     1321 atatctcact atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct
     1381 taaatctttt tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg
     1441 ttcatggtat taatcgctca gatatccacc ttttcctcag atttttttca ctaaatgcat
     1501 aaaacttaat caccaaatgt tgctaaatat taagctgatt gttctggtca tgttggagta
     1561 gcacaaattt ctctgctgca aatagctgtt tgaagttttt ttaatctttg tttgtggatt
     1621 ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt ttcacattct gttgacagat
     1681 tgtaggtcac gaggggcatt ttatggagga tccgcactgt tacactgatg ttaattagtt
     1741 cggcatcccc acctcataac gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc
     1801 gacaacccac tgcaaagggt taaaacaaca aacatcacaa ttggagcaga ataatgcgta
     1861 tttccttgaa aaagtcaggg atgctgaagc ttggtctcag cctggtggct atgaccgtcg
     1921 cagcaagtgt tcaggctaaa actctggttt attgctcaga aggatctccg gaagggttta
     1981 acccgcagct gtttacctcc ggtaccacct atgacgcctc ttccgtaccg ctttataacc
     2041 gcctggttga atttaaaatc ggcaccaccg aagtgattcc gggcctcgct gaaaagtggg
     2101 aagtcagcga agacggtaaa acctatacct tccatctgcg taaaggtgtg aagtggcacg
     2161 acaataaaga attcaaaccg acgcgtgaac tgaacgccga tgacgtggtg ttctcgttcg
     2221 atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc tggcggcagc tacgaatact
     2281 tcgaaggcat gggcttgccg gagctgatca gcgaagtgaa aaaggtggac gacaacacag
     2341 ttcagtttgt gctgactcgc ccggaagcgc cgttcctcgc tgacctggca atggacttcg
     2401 cctctattct gtcaaaagaa tatgctgatg cgatgatgaa agccggtaca ccggaaaaac
     2461 tggacctcaa cccaatcggc accggtccgt tccagttaca gcagtatcaa aaagattccc
     2521 gtatccgcta caaagcgttt gatggctact ggggcaccaa accgcagatc gatacgctgg
     2581 tcttctctat tacccctgac gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc
     2641 aggtgatgcc gtacccgaac ccggcagata tcgctcgcat gaagcaggat aaatccatca
     2701 atctgatgga aatgccgggg ctgaacgtcg gctatctttc atataacgtg cagaaaaaac
     2761 cactcgatga cgtaaaagtt cgccaggctc tgacctacgc ggtgaacaaa gacgcgatca
     2821 tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt
     2881 ggggctataa cgacgacgtt caggactaca cctacgatcc tgaaaaagcg aaagccttgc
     2941 tgaaagaagc gggtctggaa aaaggtttct ccatcgacct gtgggcgatg ccggtacaac
     3001 gtccgtataa cccgaacgct cgccgcatgg cggagatgat tcaggcagac tgggcgaaag
     3061 tcggcgtgca ggccaaaatt gtcacctacg aatggggtga gtacctcaag cgtgcgaaag
     3121 atggcgagca ccagacggta atgatgggct ggactggcga taacggggat ccggataact
     3181 tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg ctccaactac tcaaaatggt
     3241 gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc taccgacgac cataacaaac
     3301 gcgttgaact gtacaaacaa gcacaggtgg tgatgcacga tcaggctccg gcactgatca
     3361 tcgctcactc caccgtgttt gaaccggtac gtaaagaagt taaaggctat gtggttgatc
     3421 cattaggcaa acatcacttc gaaaacgtct ctatcgaata attaaaagcc atacccggtg
     3481 gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac ttcattctct gtccgacaag
     3541 ggcgggaatt gatttgtgag caatacagac acgcagttcc aggctgcggg tcattataga
     3601 gaatccgggt tatgttgcag tttattctcc gacgtttggg actcgtcatc cccacgttta
     3661 tcggtattac ccttctcaca tttgcctttg tccacatgat cccgggcgat ccggtgatga
     3721 tcatggcggg cgaacgtggg atctcccctg agcgtcacgc gcagctgctg gctgaactcg
     3781 gcttagataa accgatgtgg cagcagtatc tccattacat ttggggcgta atgcacggcg
     3841 atctaggcat ttcaatgaaa agccgaattc cagtatggga agagttcgtg ccgcgctttc
     3901 aggccacgct ggaacttggc gtctgcgcga tgatttttgc tacggcagtc ggtattccgg
     3961 ttggtgtgct ggctgcggtt aaacgcggtt ccattttcga tcacactgca gttggcctgg
     4021 cgctgaccgg ctactcgatg ccgatcttct ggtggggcat gatgctgatc atgctggttt
     4081 cggtgcactg gaacctgacg cccgtctccg gtcgcgtgag cgatatggtg ttcctcgatg
     4141 actccaatcc gttaaccggt tttatgctaa tcgacaccgc catctggggt gaagacggca
     4201 actttatcga tgccgtcgcc catatgatct tgcctgccat tgtgctgggt actattccgc
     4261 tggcggtcat tgtgcgtatg acacgctcct cgatgctgga agtgctgggc gaggattaca
     4321 tccgcaccgc gcgcgccaaa gggctaaccc gcatgcgggt gattatcgtc catgcgctgc
     4381 gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca ggtgggaaca ttgctggcgg
     4441 gggcgattct gaccgaaacc atcttctcgt ggcccggtct gggacgctgg ttgattgacg
     4501 cactgcaacg ccgcgactat ccggtagtgc agggcggcgt attgctggtg gcgacgatga
     4561 ttatcctcgt caacttgctg gtcgatctgc tgtacggcgt ggtgaacccg cgtattcgtc
     4621 ataagaagta aggggacatc atgtcacagg ttactgaaaa taaagtgatt agcgcaccgg
     4681 tgccgatgac cccgttacag gagttctggc actattttaa acgcaacaaa ggcgcggtcg
     4741 tcgggctggt ttacgtcgtc atcgtgctgt tcatcgcgat ctttgccaac tggattgcac
     4801 cctataaccc ggcggaacag ttccgcgatg cactgctcgc cccgccagcc tggcaggaag
     4861 gcggcagcat ggcgcacttg ctgggcaccg atgacgtagg ccgtgatgtg ctgtcgcgcc
     4921 tgatgtacgg tgcgcgcctg tcgctgctgg ttggctgtct ggtagttgtg ttatcgctga
     4981 ttatgggcgt tattctcggc ctgatcgccg gttactttgg cggcctggtc gataacatca
     5041 ttatgcgcgt ggtcgatatc atgctggcgc tgccaagtct gctgctggcg ctggtgctgg
     5101 tggcaatttt cggcccgtcg attggtaacg ccgcgctggc actgaccttc gttgccttgc
     5161 cgcactatgt gcgcttaacc cgcgccgccg tgctggtgga agttaaccgc gattacgtca
     5221 ccgcgtctcg cgtggcgggt gccggggcga tgcgtcagat gtttattaac atcttcccga
     5281 actgccttgc gccgctgatt gttcaggcgt cgctcggttt ctctaacgcc attctcgata
     5341 tggctgctct tggtttcctc ggcatggggg cacagccgcc aacgcctgag tggggcacca
     5401 tgctctccga cgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc ttcccgggtc
     5461 tggcgatcct gctgacggtg ctggcattta acctgatggg tgacggtctg cgtgacgcgc
     5521 tcgatcccaa actgaagcag taagaggttc gagatggcgt tattaaatgt agataaatta
     5581 tcggtgcatt tcggcgacga aagcgcgccg ttccgcgccg tagaccgcat cagctacagc
     5641 gtaaaacagg gcgaagtggt cgggattgtg ggtgagtccg gctccggtaa gtcggtcagt
     5701 tcactggcga ttatggggct gattgattat ccgggccgcg taatggcaga aaaactggag
     5761 tttaacggcc aggatttgca gcgtatctca gaaaaagagc gccgcaacct ggtgggtgcc
     5821 gaagtggcga tgatcttcca ggacccgatg accagcctta acccgtgcta caccgtgggt
     5881 ttccagatta tggaagcgat taaggtgcat cagggcggca acaaaagtac ccgccgtcag
     5941 cgagcgattg acctgctgaa tcaggtcggt attcccgatc cggcatcgcg tctggatgtt
     6001 tacccgcatc agctttccgg cggcatgagc cagcgcgtga tgatcgccat ggcgattgcc
     6061 tgtcggccaa aactgctgat tgccgatgaa ccgaccaccg cgctggacgt gaccattcag
     6121 gcgcaaatca tcgaactact gctggagcta cagcagaaag agaacatggc gctggtgtta
     6181 attacccatg acctggcgct ggtggcggaa gcggcacata aaatcatcgt gatgtatgca
     6241 ggccaggtgg tggaaaccgg tgatgcgcac gccatcttcc atgcgccgcg tcacccgtat
     6301 actcaggcat tgctgcgtgc gctgccagaa tttgctcagg acaaagaacg tctggcgtcg
     6361 ttgccaggtg tcgttcccgg taagtacgac cgcccgaacg gctgcctgct taacccgcgc
     6421 tgcccctatg ccactgacag atgtcgcgct gaagaaccgg cgctgaatat gctcgctgat
     6481 gggcgtcagt ccaaatgcca ttacccactt gatgatgccg ggaggccgac actatgagta
     6541 cgcaagaggc cacctcgcaa caaccgctgt tgcaggctat cgacctgaaa aaacattatc
     6601 cggtgaagaa aggcatgttc gcgccggaac gtctggttaa agcgctggat ggcgtttcgt
     6661 ttaaccttga acgtggcaaa acgctggcag tagtgggcga atctggctgc ggtaaatcga
     6721 ccctcggtcg gttgctgacg atgattgaaa tgcccaccgg tggcgagctg tattaccagg
     6781 ggcaggatct gcttaagcac gatccgcagg cgcagaagct gcgtcggcag aaaatccaga
     6841 tcgtcttcca gaacccttac ggttcgctga atccgcgtaa aaaagttggg caaattctcg
     6901 aagagccgct gcttattaac accagcttaa gcaaagaaca gcgtcgggaa aaagccctgt
     6961 cgatgatggc gaaagtcggc ctgaaaaccg agcattacga ccgctatccg cacatgtttt
     7021 ccggcggaca acgtcagcgt atcgctattg cccgtggtct gatgctcgac ccggacgtgg
     7081 tgattgccga tgaaccggtt tctgcgctgg acgtctcggt gcgcgcgcag gtgctcaacc
     7141 tgatgatgga tttgcagcag gaactggggc tttcttatgt ctttatctcg cacgacctgt
     7201 cggttgtgga gcacattgcc gatgaagtga tggtgatgta cctgggccgc tgcgtggaga
     7261 aggggacgaa ggaccaaatc ttcaataacc cgcgtcatcc ttacactcag gcgctgctct
     7321 ccgcgacgcc gcgcctgaac ccggacgatc gccgcgagcg catcaagctc accggtgaac
     7381 tgccaagccc gctgaatcct ccgccgggtt gcgccttcaa cgcccgctgt cgtcgacgct
     7441 tcggcccctg cacccagttg cagccagcac tgaaagatta cggcggtcaa ctggtggcct
     7501 gctttgccgt tgatcaggat gaaaatccgc agcggtaatg ttcaaaaccg gagccttgtg
     7561 ctccggttta ctcaatcagc ttcaacagtg gcgacaagca tagcaaaatg ccgtacagca
     7621 gaatcagcca ggctttaaat ccgcgaagtt tttccagttg tgcgacttta tagatcagga
     7681 aaaacggaat gaggcacgac acaatgccat ataacgggct tcccaactga aagaacacca
     7741 gcaccgatac acgaaacgaa acccaaatcg tcaacgtaat gacgataaaa gcgcagatcg
     7801 ccagagtcag cacgcgtgag ttaattttct tggtatcaat aatacggctt aacagattga
     7861 gaataatgcc tttaatggcc tcgtggaaac cgagataaat gccaaagaat gcggtcagta
     7921 cggcaaagat attcagtacc gtagaggtga tatgaatgat atgcccaggg atcacctgcg
     7981 cggccagtgc cagtgccgag atattttgtt caaaggcaga aacggcttct tcgtggctaa
     8041 ttgagaaggt aaacgaaaag gcaaaaaaca gtatcaccgc gatgagcgtg acataactaa
     8101 ttcggtgggt acgcagcgcg agccgggttg ccagtacttt atccgcttcc cgtttacgat
     8161 aggcaatatt cattgggtta agtacctgaa taaataccgc agaaaagaag caaaagggaa
     8221 tggtaagcag gacatcgcgg aaaaagacgg aggcttgcgg gaaggcggtt atattggcga
     8281 aattccagtg cgggatcatc gcaaaaccga acacgacaat aatccctact ttgaccacca
     8341 ccattggccc agaaatctta aacagtaatc gttcaccacc agacgcaatc gccaccagta
     8401 cggcgaaaat agcgacttta taaagtagag attgtgaaag atcggcatcg gttaaaccga
     8461 aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag agagtagata aaaatcccgt
     8521 ggataatcat caaaaagtag ataaccccga ggaaaattcc ccagttcttc cccaggtaat
     8581 gactgataat atcggtgtag tcattacagg aatcgctttc agaaagggtt tttaaataaa
     8641 tgtcctgcac tacccaagtg gcaggataag caatgatcgc tgcggtaata aatacccaaa
     8701 ttcccttcaa gccaatttgt actggcatca gcacggttcc ggcaccaatc gccatgccta
     8761 tgcataataa aacccagccg aagtcgtagc gtgtaaacgg caatttctga tcgtgtttcg
     8821 gtagtgtgtt gtgctgcata aagtcctgtc cagaaagaag tcggcctcgc aaaaatgaat
     8881 aaacccagat tctcttgcgc aacaggtgga gaagtcgaat acgcagtatg taaaaaatga
     8941 ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt ctacaaaatt gcgcatttca
     9001 ctaccagtct ggagtcagcc cggatatgaa ctgcaactat tgcaaattaa gaaattaagc
     9061 tggaaggcat cacgcaatga ggaagattgc cgggtgctga cagtaaaaag caatcgtaaa
     9121 atcagcggca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
     9181 tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatatac ctgacaacat
     9241 aacgggggct aatcttgaac ctagaccact caagaatagc cgcgaaacgt tgtcattaca
     9301 acacaggcgg ctatatgacg ctcgcagagc tgggcatggc cttctggcat gatttagcgg
     9361 ctccggtcat tgctggcatt cttgccagta tgatcgtgaa ctggctgaac aagcggaagt
     9421 agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag
     9481 gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt
     9541 ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa aaacagtcgt
     9601 agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg gcttaatccc
     9661 tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa
     9721 caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa cgttgtcatt
     9781 acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag
     9841 cggctccggt cattgctggt attcttgcca gtatgagcgt gaactggctg aacaagcgga
     9901 agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg
     9961 caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca agcggataat
    10021 attctgcgga atgcgcagca cagagtgagt aagtgatgga ttccggaaca gaaaaacagt
    10081 cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg ggggctttat
    10141 ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt atgcacctga
    10201 caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg aaacgttgtc
    10261 attacaacac aggcggctat atgacgctcg cagaactggg catggccttc tggcatgatt
    10321 tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg ctgaataagc
    10381 ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc ccggatcagg
    10441 ccgcagaggt taaactctgc ggcctttttc gtgcttactg cgggtaaggc acccagtcgc
    10501 cgccgttcag gcgaacgtac ggtttatcct ggtattgaat aactactgca tttgagttct
    10561 cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca acattgtctt
    10621 cggtgaaaat cttgccatca agaacgcgaa ccaccagatc ggagatagcc aggaagctgc
    10681 tcggttggtc aatgacaatc ggtgccccct gatgtggtgc cttcatgccg aagaatttca
    10741 ccccaacggg gacgtctgtg atagacgggc tagggatatc acgtaggcca gatacctgca
    10801 ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc actttacgac
    10861 ccgatttctc cagttcagta aagaaggcgt ccagttcatc aaagaatttc tgcgcccgcg
    10921 ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc agtggaagcg
    10981 tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc agccagcggt
    11041 taagcacggc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa ataaccggca
    11101 gatttgtttg atccatcaat tcagtctgca tgccgccatt ttcgcgaact tctttcaaaa
    11161 aaccgccgaa ctggccgtta tgccccatca tcaggtgctg ggtaaagccc agtttcgaaa
    11221 ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg tgcgaagtct
    11281 gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag gtggcggagt
    11341 taaagttctt gaactcaata tcgaaatgcg accacagtgg atgcgacatc aacccggcgg
    11401 cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca aatggctgag
    11461 catcagcggg cagcgaagac gggaaggtcg atttacgttt tgcctccgcg ttatagaaat
    11521 tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg ggcatatcac
    11581 ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca cccgtcgttg
    11641 ttacagtggt cgtcggttgt ccggctggcc acaaggagaa acttggtccc gccagggtaa
    11701 gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc cattgtgaca
    11761 ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag ttaataaagc
    11821 gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa ccctggctca
    11881 ttatgctttc cgggccaggc aaccaggtgt catgccagaa caaagcaaag ccgatcggca
    11941 gggcaatcca gtggcgcaag cgatgcaggc tgtagcgcgg aatgggcatc agcagaaacg
    12001 cggcaaacac caaattgagg agcggatgga agttaagata tcccgcccac aacaggccga
    12061 acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc cagagggaag
    12121 aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg ctttttccgt
    12181 gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca aacgtaaggt
    12241 gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa atatcagtgc
    12301 gcaaacgaca ataatttcaa tgatatcgct gatggtcatc atgatgagcg ctccacagca
    12361 tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc gtgctcggca
    12421 ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca tgccccattg ttctggggca
    12481 agcaagcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca gaccatacgg
    12541 tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt atccagatcg
    12601 ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt catgatatcg
    12661 ccggtacggt tagggcgaca cagcgtcagg gcttgttcaa cgcggatacc cggtaccgga
    12721 cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga gagggttatt catcatgttg
    12781 ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag cggctgggtc
    12841 attgacagca aggtagtgat atcttccggc acatagcgac taaacttctg cccttgcacg
    12901 ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac catatttgca
    12961 ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt ttcccgcacg
    13021 aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg ggccagtggc
    13081 tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc ggtacgggct
    13141 tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag cggcggcgca
    13201 ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg ttggatctcc
    13261 gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct aagctgctga
    13321 cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg ttggtcaccc
    13381 tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctcaagcaa caatgaaaat
    13441 tgtttatcgt tattatttcc gggattaatt accaaaagcg aacaatggtt taacctgctc
    13501 catttattca ttttatccaa ccatgagcga agccgctcgg caggaatgtt ttgccatgcg
    13561 ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat caatagaaca ctgcaaatca
    13621 cgggtcaaat agtatagacc tttttcatga tttagcattg aaaataattt tattttttcc
    13681 ggcccttgag aatcatctaa ttgaaagatt ttcgccggat cgctgtccat gctaatgacc
    13741 gcgacgtgtg cggttgcagc ctgggatgca attgtttgat tcgccagact gatagcatct
    13801 tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg cagctcatcc
    13861 cataatgatg agataccgat agagaataca gggtccacaa tgtccctcat gtcaccattt
    13921 tgtctgatta tcagtttact agcgaaagct aagaaataaa cctaccattg aaattaaaac
    13981 ttatcagatt tagcatgtga atcaatttag aaggtcacaa gacgtgtgat tttagtcttt
    14041 tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa ttcccgtctc
    14101 gccgtaaaca ataaaccggc gaaatgctca cggttagaat tgtctcatga acggtacggt
    14161 tatttcatag ggatcaagca aaatgaataa caatgaacca gatactctgc ctgatcccgc
    14221 gataggctat atcttccaga atgatattgt ggcgttaaag caggcatttt cactgcctga
    14281 tattgattat gccgatattt cccaacgcga acagttggcc gcggcattaa aacgctggcc
    14341 gttgctggca gagtttgcgc aacaaaagta ggggattggt gaatggccgt actgggattg
    14401 cagggggtgc ggggaggcgt ggggacaaca accatcaccg ccgcattagc ctggtcatta
    14461 caaatgttgg gagaaaatgt tctggtggtc gatgcctgcc cggacaactt gttgcgcctg
    14521 tcatttaatg ttgattttac ccaccgtcag ggctgggcca gagcgatgct ggatggccag
    14581 gactggcgtg acgctgggtt gcgctacacc tcgcagctcg atttgctgcc ttttggtcag
    14641 ttatccattg aagaacaaga aaatccacag cactggcaaa cccggctgag cgatatttgc
    14701 tccggcttac agcaactaaa agccagcggg cgttaccagt ggattttaat cgacttaccg
    14761 cgtgatgcct cgcagataac ccaccagctg ctgagtttgt gcgatcactc gctggcaatc
    14821 gtcaatgtgg atgccaactg ccatatccga ctgcatcagc aagcgctgcc ggatggcgca
    14881 catattttga ttaatgactt ccgtattggc agtcaggttc aggacgatat ttaccagctt
    14941 tggttgcaaa gccagcgccg attactgccg atgctcattc atcgtgatga agcgatggct
    15001 gaatgcctgg cggctaagca accagtaggt gaatatcgca gtgatgcgct ggcggctgaa
    15061 gagatactga cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa aacgccagtc
    15121 gggagtgcat catgagtatc ctgacccggt ggttgcttat cccgccggtc aacgcgcggc
    15181 ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc gcgacgctcg
    15241 gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg cgctggcagc
    15301 gtattcgcgc agaacataaa aacctgtatc cgcatatcaa cgcctcgcgt ccgcgtccgc
    15361 tggacccggt ccgttatctc attcaaacat gctggttact gatcggtaca tcgcgcaaag
    15421 aaacgccgaa accgcgcagg cgggcatttt caggtctgca gaatattcgt ggacgttacc
    15481 atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat cttgatgaga
    15541 aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt atcatcgtca
    15601 ccttctcgct gattctggcg ttaatctgcg ttactcaacc gtttaacccg ctggcgcagt
    15661 ttatcttcct gatgctgctg tggggtgtag cgctgatcgt acggcggatg ccggggcgct
    15721 tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat atctggtggc
    15781 gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg cttattctgc
    15841 tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag gtagtatggc
    15901 cgctgaatcg tcagccggtg ccattgccga aagatatgtc gctgtggccg tcggtggata
    15961 tctttgtccc gacttacaac gaagatctca acgtggtgaa aaatactatt tacgcctcgc
    16021 tgggtatcga ctggccgaaa gacaagctga acatctggat cctcgatgac ggcggtaggg
    16081 aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgt accactcatg
    16141 aacatgcgaa agcgggcaac atcaacaatg cgctgaaata tgccaaaggc gagttcgtgt
    16201 cgattttcga ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc atgggctggt
    16261 tcctgaaaga gaaacagctg gcgatgatgc agactccaca ccacttcttc tcgccagacc
    16321 cgtttgaacg caacctgggg cgttttcgta aaacaccgaa cgaaggcacg ctgttctatg
    16381 gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt tcctgtgcgg
    16441 tgattcgccg taagccgctg gatgaaattg gcggcattgc tgtcgaaact gtgactgaag
    16501 atgcgcatac ttctctgcgt ttgcaccgtc gtggatatac ctccgcgtat atgcgtattc
    16561 cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag cgtattcgct
    16621 gggcgcgcgg gatggtgcaa atcttccgtc tcgataaccc gctcaccggt aaaggactga
    16681 agtttgctca gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg ggcattccac
    16741 ggctgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac atcatctatg
    16801 cgccagcgtt gatgatcgcc ctattcgtgc tgccgcatat gatccatgcc agcctgacca
    16861 actcgaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac gaaacggtgc
    16921 tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac aaaggcaaat
    16981 ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg gtgatctcgc
    17041 ggccctacat cttccttgtt ctactcaacc tggtgggcgt tgcggtaggc atctggcgct
    17101 acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg tgggtgttct
    17161 acaacctgat tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa caggtacgcc
    17221 gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat ggtcacctct
    17281 tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc aacggtcagg
    17341 cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag caggaatacg
    17401 tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa ttaatgccgc
    17461 tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg gatacatggg
    17521 cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg gatattctga
    17581 agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg gtgaagggca
    17641 tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg cgccgcccgg
    17701 agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga tgataacgcg
    17761 atgaaaagaa aactattctg gatttgtgca gtggctatgg ggatgagtgc gttcccctct
    17821 ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca atgctgagcc agctgtagcc
    17881 gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc cgggcgtgca gggcgctgat
    17941 gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga agctgacctt tgcgcaaatt
    18001 gcgccgccgc cgggcagcat ggtgctacgt ggcattaacc cgaacggcag cattgagttt
    18061 ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca acctcgaata cacgccatcg
    18121 ccatcgttac tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga gctgatgggc
    18181 gtgctgccag tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat gcccattaac
    18241 ccactgttta ttaaagactt caaccgtgtg cggctggagt ttgtcggcca ttatcaggac
    18301 gtgtgcgaaa acccggccag caccacgctt tggctggatg ttgggcggag cagtggactg
    18361 gatctgacct atcagaccct gaatgtgaag aatgacctgt cacacttccc ggtgccattc
    18421 tttgatccgc gtgataaccg caccaacacc ttgccgatgg tctttgcggg tgcgccggat
    18481 gttgggctgc aacaagcctc tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg
    18541 cgtgggcaga acttcccggt gctctataac caactgccgg atcgtaatgc cattgtcttt
    18601 gcaaccaacg acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa agccccggtg
    18661 attgagatga ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt
    18721 gacgacaaag acctgttgca ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt
    18781 ggtgaaagcg tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg
    18841 ccgaactggg tacgtaccga tcgtccggtc acatttggcg aactgaaaac ctatgaagaa
    18901 cagttacaat ccagcggtct tgagccagca gcgattaacg tttcgctaaa cctgccgccg
    18961 gatctctacc tgatgcgcag taccggcatt gatatggata ttaattaccg ctacaccatg
    19021 ccgccggtga aagacagttc gcggatggat atcagcctga ataaccagtt cctgcaatcc
    19081 ttcaacctga gcagcaaaca ggaggcgaac cgcctgctgc tgcggattcc ggtattacaa
    19141 ggtttgctgg atggcaaaac agatgtctct attccggcgc tgaaactggg cgcgaccaac
    19201 cagctgcgct tcgactttga gtatatgaac ccgatgccgg gcggttcggt ggataactgt
    19261 attaccttcc agccggtgca gaatcatgtg gtgattggtg acgactccac catcgacttc
    19321 tcgaagtatt accacttcat cccgatgccg gatctacgcg cctttgctaa cgcgggcttc
    19381 ccattcagcc ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa agcgcctaac
    19441 gaagcacaga tggaaacgtt gctgaatact gttggtttta tcggcgcaca gacgggcttc
    19501 ccggcgatta atctgacggt gaccgatgat ggcagcacca ttcagggcaa agatgccgac
    19561 atcatgatca tcggtggtat cccggacaaa ctgaaagacg ataagcagat cgacctattg
    19621 gtgcaggcga ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt
    19681 gtgccggacg agagcgatcg cgcggcagaa acccagtcaa cgctgacctc ttccggtgcg
    19741 atggcggcgg tgattggctt ccagtcgccg tataacgacc agcgcagcgt gattgcgctg
    19801 ctggcagata gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa
    19861 cgcgccacca tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa cagcctacgt
    19921 gttggcgacg tttattacgt aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg
    19981 gcaaaccatc cgattctgct ggcggtgctg gcggctatca gtgtgatatt gctggcatgg
    20041 gtactgtggc gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga taacgagtaa
    20101 ttgaagatga atgtgttgcg tagtggaatc gtgacgatgc tgctgctggc tgcctttagt
    20161 gttcaggcag cctgtacctg gcctgcctgg gagcagttta aaaaggatta catcagtcag
    20221 gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca ccacctccga agggcaaagt
    20281 tacggtatgt tctttgccct ggcggctaac gaccgtgtag ctttcgataa tattctcgac
    20341 tggacgcaga acaatctcgc tcagggctct ttaaaagaac gtttgcccgc ctggctgtgg
    20401 ggcaagaaag agaacagtaa gtgggaagtg ctggacagca attcggcctc cgatggtgat
    20461 gtctggatgg cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca gcgttatacc
    20521 gacatcggca gcgcgttgct aaaacgtatt gcgcgggagg aagtggtgac ggtgcctggg
    20581 ctgggttcca tgttgttacc gggcaaagtg ggttttgctg aggataacag ctggcgtttt
    20641 aaccccagct acctgccgcc gacgctggcg cagtatttca cccgctttgg cgcgccgtgg
    20701 accacgctgc gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa aggtttttcg
    20761 ccagactggg tgcgctatga gaaagacaaa ggctggcagc taaaagccga aaaaacattg
    20821 atcagcagct acgacgccat ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat
    20881 ccgcagaaag cgcggatgct caaccggttt aaaccgatgg cgacattcac tgagaaaaac
    20941 ggttatccgc cggaaaaagt ggatgtggct acggggaaag cgcagggtaa aggaccagtc
    21001 ggtttttctg ccgccatgct gcccttttta caaaaccgcg atgcgcaggc cgttcagcgc
    21061 cagcgcgtgg ccgataactt tcccggcagc gatgcctatt acaactatgt gctgaccctg
    21121 tttggacaag gctgggatca acaccgtttc cgcttctcga caaaaggtga gttattacct
    21181 gactggggcc aggaatgcgc aaattcacac taaacatatt cacgctttcc ctcggtctgg
    21241 ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca acagttgctg gagcaagttc
    21301 ggttaggcga agcgacccat cgtgaagatc tggtgcaaca gtcgttatat cggctgaaac
    21361 ttattgatcc gaataacccg gacgtcgttg ccgcccgttt ccgttctttg ttacgtcagg
    21421 gcgatattga tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg ccgagttcaa
    21481 atgcgtataa atcgtcgcgg actacgatgc tactttccac gccggatggt cgtcaggcac
    21541 tgcaacaggc acgattgcag gcgacgaccg gtcatgcaga agaagctgtg gcgagttaca
    21601 acaaactgtt caacggtgcg ccgccggaag gtgacattgc tgtcgagtac tggagtacgg
    21661 tggcgaaaat tccggctcgc cgtggcgaag cgattaatca gttaaaacgc atcaatgcgg
    21721 atgcaccggg caatacgggc ctgcaaaaca atctggcgct attgctgttt agtagcgatc
    21781 gccgtgacga aggttttgcc gtcctggaac agatggcaaa atcgaacgcc gggcgcgaag
    21841 gggcctctaa aatctggtac gggcagatta aagacatgcc cgtcagtgat gccagtgtgt
    21901 cggcgctgaa aaaatatctc tcgatcttta gtgatggcga tagcgtggcg gctgcgcaat
    21961 cgcaactggc agaacagcaa aaacagctgg ccgatcctgc tttccgcgct cgtgcgcaag
    22021 gtttagcggc ggtggactct ggtatggcgg gtaaagccat tcccgaacta caacaggcgg
    22081 tgcgggcgaa cccgaaagac agtgaagctc tgggggcgct gggccaggcg tattctcaga
    22141 aaggcgatcg cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg gacccgcaca
    22201 gcagcaacaa cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg ctggcgatcc
    22261 agcagggcga tgctgcgctg aaagccaata atcctgaccg ggcagaacgc ctgttccagc
    22321 aggcgcgtaa tgtcgataac accgacagtt atgcagtgct ggggctgggc gatgtggcga
    22381 tggcgcgaaa agattatccc gccgccgaac gttattatca gcagaccttg cgtatggaca
    22441 gcggcaacac taacgccgtg cgcgggctgg caaatattta ccgccagcaa tcgccagaaa
    22501 aagctgaagc gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt gatgatatcg
    22561 aacgcagcct gcaaaacgac cgtctggcac agcaggcaga ggcactggaa aaccagggca
    22621 aatgggcgca ggcggcagca cttcagcggc aacgactggc gctggacccc ggcagcgtat
    22681 ggattactta ccgactttcg caggatctct ggcaggccgg acaacgcagc caggccgata
    22741 cgttaatgcg caatctggcg cagcataagc cgaacgaccc ggagcaggtt tacgcttacg
    22801 ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc aacagcctgc
    22861 cgcgcgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa agcgatcagg
    22921 tgctggaaac cgctaatcgc ctgcgagaaa gcggcaaaga agctgaagcg gaagcgatgc
    22981 tgcgtcagca accaccttcc acgcgtattg acctcacgct ggctgactgg gcgcaacaac
    23041 gacgtgatta caccgccgcc cgtgctgcat atcagaatgt cctgacgcgg gagccagcta
    23101 acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac aaagcggcgg
    23161 cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac acacagcggc
    23221 gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg acgtttaata
    23281 agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg gtgctgcgcg
    23341 acggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa acctacaaag
    23401 acgccatggt cgcatccggt gtgaccacga cgcgtccgca ggataacgac acctttaccc
    23461 gactgacccg taacgacgag aaagatgact ggctgaaacg cggcgtgcgc agcgatgcgg
    23521 cggacctcta tcgccagcag gatcttaacg tcaccctcga gcacgattac tggggttcga
    23581 gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag gtggatgcgc
    23641 cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac gtcggcagtt
    23701 tctccactaa tgccgatggt aaatgggatg acaactgggg cacctgtaca ttacaggact
    23761 gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc tggcgaaatg
    23821 acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg gatgtggtcg
    23881 gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac gcccaccgtc
    23941 ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg agcaataccg
    24001 ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc tacgataaag
    24061 gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt aaaaatgtcg
    24121 aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt aaccagaaca
    24181 atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa gatctgagtg
    24241 gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg tttgccatac
    24301 cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg tctggctcgt
    24361 ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg atcccgaccg
    24421 actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag ggcttcggct
    24481 acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt ggcacggcaa
    24541 ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac gtacgttatt
    24601 ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata ccttacgccg
    24661 actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta tactcgggcg
    24721 gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta acaatcaagc
    24781 agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc accgttttgc
    24841 tgttccatct ggtccaacag aatcgctata acacggctac gcaactggaa agcattgctc
    24901 gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc gaagcggaag
    24961 ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta gtgctgccta
    25021 accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg gtaatggtta
    25081 ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc gaacgtccgg
    25141 caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt atgtataagt
    25201 tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg cttatcctga
    25261 ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc aatattgctc
    25321 gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca ttaccgcgtc
    25381 tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac cagcaattgc
    25441 tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg tcggatttgc
    25501 cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa caaaccaccg
    25561 cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg aaagaggcgc
    25621 aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg tcgccacgta
    25681 tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt gtacaggaac
    25741 cgtggcacgc aatcacctta ggtcagcaag tgctcactat catgagcgag cgcctgccga
    25801 ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc tacggcgatc
    25861 tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct cgccataaag
    25921 gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag aagcggttga
    25981 cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgcaatt tggttacagc
    26041 cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg cgtatccagc
    26101 aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag tgctgtgggc
    26161 tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt gcagcctggc
    26221 aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa ctgatgcacc
    26281 caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag ccgggaacac
    26341 tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg gtggcaatcc
    26401 tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc atgggctacg
    26461 cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg aaaatcgaca
    26521 aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt atcatgctgg
    26581 cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca caacgcgact
    26641 ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcgc ccactcccta
    26701 ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact gattacaaaa
    26761 ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt tgtttcaatt
    26821 atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg caggtacccc
    26881 ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct ctctgtttaa
    26941 aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg gccatttcta
    27001 tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc tcattaagat
    27061 gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca tggaaagcat
    27121 gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg tcagtaccat
    27181 cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg gaatgaacgt
    27241 cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg cgaaagacca
    27301 gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg catttgccag
    27361 cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc tccaccgtct
    27421 gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg tcatcttcgg
    27481 catcatcaat atgatcatgc gtctggcgcc tattggtgcg ttcggggcaa tggcgtttac
    27541 catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta tctgtttcta
    27601 cattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag cgactggttt
    27661 cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac tggggacttc
    27721 atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg gctgccgcaa
    27781 atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg gcacatcgat
    27841 atacctgaca atggcggcgg tgtttatcgc ccaagccact aacagtcaga tggatatcgt
    27901 ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg caggggtaac
    27961 gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt tgccggtagc
    28021 gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg cgctgactaa
    28081 cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag aactggacca
    28141 caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc acgaattatc
    28201 ctcttaatct catcacttat gcccgcactc ccttccccga gtgcgggtgt ttgcgcataa
    28261 ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca cttcttccgt
    28321 gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt agactgaccg
    28381 acaacgggca gtttttttat taccaggata gttgatcagg ggttcacatg cagggcacaa
    28441 aaattcgact tttagcgggc ggtttgctga tgatggccac tgctggctat gtgcaggcag
    28501 atgcgctcca gcctgatcca gcatggcaac aggggacgct ttccaacggt ttacagtggc
    28561 aagtgctgac caccccccag cgtcccagcg atcgtgttga aattcgcctg ctggttaata
    28621 ccggttcgct cgccgaaagt acacaacaga gcggttacag tcacgccatc cctcgtattg
    28681 cgctaacgca aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg cagcagggga
    28741 tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta tgacaccacg ctgtttaatc
    28801 tgagtttgcc caataaccgt aacgatttgc tgaaagaagc gctctcttat ctggcaaatg
    28861 ccactggcaa attgaccatc acaccagaaa ccatcaacca cgcgctgcaa agtcaggaca
    28921 tggtggcaac ctggcctgcc gatactaaag agggctggtg gcgctatcgt ctgaaagggt
    28981 caaccttgtt aggtcacgat cctgccgatc cgctgaaaca acccgttgaa gcggaaaaaa
    29041 ttaaagattt ctatcagaaa tggtacaccc cggatgcaat gacgctactg gtggtgggaa
    29101 acgtggatgc gcgctcggtt gtcgaccaaa taaacaaaac gtttggcgaa ctgaaaggca
    29161 aacgtgaaac gccagctccg gtgccgacgc tttctccgct gcgtgcggaa gcggtgagta
    29221 ttatgactga cgcggtgcgt caggaccggt tatctatcat gtgggatacg ccgtggcagc
    29281 cgattcgtga atcagccgca ctgctgcgct actggcgtgc ggacctggcc cgcgaggcgc
    29341 tgttctggca tgttcagcaa gcgttaagcg ccagtaacag caaagacatc ggtcttggat
    29401 ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgaa tcaccaaacg
    29461 acaagctgaa cagcaacctt aatctggtgg cgcgtgaact ggcgaaggtt cgcgataaag
    29521 gtctgccgga agaagagttc aatgcgttag tggcgcaaaa gaaactggag ctgcagaaac
    29581 tgtttgccgc ctatgcgcgg gctgataccg atattctgat ggggcagcgg atgcgttcgt
    29641 tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg caggatttcc
    29701 ttaacagcct gacggtggag atgttaaatc aggatctgcg tcagcagttg tcgaatgata
    29761 tggcgttaat actgctgcag ccgaaaggcg agccggaatt taacatgaaa gcgttgcagg
    29821 cggcctggga tcaaatcatg gccccatcga ctgctgctgc cgccacctct gtcgccacgg
    29881 atgacgtaca tcctgaagtg acggatattc cacctgcaca gtaatgcctg atggtgatga
    29941 gttagcacaa ccatcaggta tttatggatg agctggatag aggggtaaca ctttatccag
    30001 ccttttgcat atgctgcgtt tacgctggca tcgcctcacg cgggataatc gcgccgcgat
    30061 actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc cgcgttttcc gcactgccgc
    30121 ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc gccagctgcg gtggtatcga
    30181 tcactttttc tttcggcagt ttcaccgccg gaacatccac taacccttcg ccagcaatgg
    30241 acaccaggca agaatccgcc ccgcgtttca ccaccacttc tttcacgccc gcgttatggg
    30301 tgcgcgcaat gacgtcttcc accggctgtt gaccccacag cgcgtcttca tcgtccagcg
    30361 tcaggaaggc gatatccgtg cattccagca tttgttggta cacctgctgt gtctcttctt
    30421 tgctggccca caggcgcgga cgatagttat tgtcgaaaat cacttttccg ccgttggcgc
    30481 ggcattcgcg cagcagggaa agcagctttt cgcggctggt cgggcttaag atcgccaggc
    30541 taatcccgct caggtagaga taatcgaaat tcgccagctc ttcgcaaatc gccgcagact
    30601 gctcgctctc cagccagaat ttggcagcgg cttcgttacg ccagtagtag aacgtacgtt
    30661 caccggtgct gtcggtttca atgtagtaaa ggcccggcag acggttttcc atccgttggg
    30721 tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag catttgttgg ctaaaactgt
    30781 ccgttcccag cgccgttacg taatggacgg ttaatgccgc aggatcgacc tgacgggcga
    30841 tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc gcgcctttct
    30901 cggaaagctc aatcatgcat tcgccaatca cggcaatctt tttggacata gtcgtgaacc
    30961 tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca accatattaa
    31021 aacagtgttc cattattttt ttgagccagg acgtattcct gaaagattcc gttgtggagt
    31081 gaggaaattt accccggcca ggcggtaaag ttctgccctt acgcgccgat aatctttgtc
    31141 gagtccgggc agcatcactt ttaaacacag gacatctttg atgataaggc aggttatcca
    31201 gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt tttggttgca
    31261 gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcgggcggt taatggccgt
    31321 ggagctatta acggtggtcg cgcatccctt gaacccttcg caacgcctgc cgccggatcg
    31381 ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc agattgattt
    31441 gctggcgcaa aaagccgact tctttataga gcacggcctg ctggcatcgg tcaatattga
    31501 tggccctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaga ttgagcgtct
    31561 tccctggctg cgtttcgaac tggtggagca tatccgtctg ccgaaagatt caacctttgc
    31621 ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggtaccggga tggcaaattt
    31681 ctctgcacta agtgaagtgc gttatgacta catcaaaatc gcgcgagaac tgtttgtgat
    31741 gctgcgtcag tcgccggaag gacgcacact cttttctcag cttttacatc taatgaatcg
    31801 ctattgtcgc ggggtgattg ttgagggcgt agaaacgccg gaagagtggc gtgatgttca
    31861 gaactcgccc gcattcgccg cacaaggctg gtttctttca cgcccggcac cgatagaaac
    31921 gctgaatacg gcggttctgg cgctataagc tgcctcattt tccgcctggc tggactatct
    31981 ttaggactgg cacaggaaga atatgaggaa gcgaacgatg agcaaggcag gcaaaataac
    32041 cgctgcgatt tcagtggctt tcttgttgtt gattgtcgtg gcgatcattt tgattgcaac
    32101 ttttgactgg aatcgactca aaccgaccat caaccagaaa gtctctgcgg agttgaatcg
    32161 tccgttcgct atccgtggcg atctgggcgt ggtgtgggag cggcaaaaac aagaaactgg
    32221 ctggcgcagc tgggtgccgt ggccccatgt acacgcggaa gacatcattc ttggcaatcc
    32281 accggatatt cccgaagtca cgatggtgca tttgccacgc gtagaggcaa cgctggcccc
    32341 gctggcgctg ctgaccaaaa cggtctggct gccgtggatc aagctcgaaa agcccgacgc
    32401 gcgcctgatt cgcctctccg aaaagaacaa taactggacg tttaatctcg ccaacgatga
    32461 taacaaagac gcgaatgcaa agccgtcggc atggtcgttt cggctggata atattctttt
    32521 cgatcaaggg cggatcgcta ttgatgacaa agtaagcaaa gcggatctgg agatttttgt
    32581 tgatccgtta ggcaagccgc tgccgttcag cgaagttact ggatcgaaag gtaaagcgga
    32641 taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt ataacggtga
    32701 accgctcacg ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg aagggacgcc
    32761 gtttccggta caggctgatt tccgctctgg taacacccgt gttgcttttg atggcgtcgt
    32821 gaatgaccca atgaagatgg gcggtgtcga tttacggctt aaattttctg gcgattcact
    32881 gggtgatctc tatgaactga cgggcgttct gctgcccgat accccgccgt ttgaaacgga
    32941 tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt atcgcggttt
    33001 taatgggcga attggcgata gcgatatcca cggttctctg gtctacacca ccggaaagcc
    33061 acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg acctgggacc
    33121 gttaattggc gttgactccg ggaaaggggc agaaaagtcg aaacggtctg aacagaagaa
    33181 gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct tcgaaaccga
    33241 taaatgggac gttatggatg ccgatgttcg cttcaaaggg cggcgcattg agcatggcag
    33301 tagcctgccg attagcgatc tttctactca tatcatcctc aaaaatgctg acctgcgcct
    33361 gcaaccgctg aaatttggca tggcgggcgg cagcattgcg gcgaatatcc atctggaagg
    33421 cgataaaaag ccgatgcagg ggcgggcaga tattcaggct cgtcgactga aactgaaaga
    33481 actgatgccc gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg gtgacgcgga
    33541 actacgcggt agcggtaact cggtggcggc gcttttaggc aacagtaacg gcaacctgaa
    33601 actgttgatg aatgacgggc tggtgagccg caacctgatg gagattgttg ggctgaatgt
    33661 cggcaactac attgtcggtg cgatatttgg tgacgatgag gtgcgggtga actgcgcggc
    33721 ggcgaatctg aatattgcca acggcgtggc gcgcccgcag atttttgctt tcgatactga
    33781 gaacgcgttg attaatgtta ccggcacggc aagttttgct tcggaacagc tggatttgac
    33841 tattgatccg gagagtaaag ggattcggat tatcacactg cgttcgccgc tgtatgtgcg
    33901 tgggacgttt aaaaatccgc aggctggggt gaaagccggg ccgttgattg cccgtggtgc
    33961 tgttgctgcg gcactggcaa cgctggtaac accggcggcg gcgttactgg cactgatctc
    34021 accttccgaa ggggaggcta atcagtgtcg gacgatattg tcgcagatga agaagtgatg
    34081 aggttggatt gtgtttgctg gtgagttcgg gagcagcgtg ttcccctcac cctaaccctc
    34141 tccccaaagg ggcgagggga ctgttttgtg cgtgttgaaa caacgagtac cgcttttccg
    34201 ttcactttct gggggagggc tgttatgttc gcgatataac atctcgctcc gcttttctcc
    34261 ctctcccttt cagggagagg gctggggtga gggtggctgc ccacacgata cttacaacga
    34321 ctgatgtcgc gtctcatggg tcagcagcag ggcgattaac gtcaagccag ccatcgccgc
    34381 cagatataac cccaccgcac ctaacccgta gttagtctgc aaccaggctg cgatatacgg
    34441 cgcaacggaa gccccgagaa tcgacgctac gttgtaagag aacgatgctc cggtgtaacg
    34501 cacttctgtt gggaacagct ctggtaacag cgcgcccatc ggcccgaagg tcagccccat
    34561 cagacttaac cccagcagca ggaaggcaaa aaccagaatc gggttgccag aaccgagcag
    34621 cgggttaaag gcgaacagcg cgaacaggat gatcagcgtg gtgataatta ccatgctttt
    34681 acggcgacca aaggcatcag ccagtaatcc ggcgactggc accatcacgc caaaaccaat
    34741 aactgccatc atcagcatcc acaacacttc gttacgtggc aggccaagcc caactggcgc
    34801 ggcggcggta ctgaaggtca tagagtagac cgtcatgatg taaaacagcg tatatgttgc
    34861 cagcatgatg aacgtaccca gtacggttac gcgaacatgt ttggtcagca gcgtacccag
    34921 cgggatcttc acctgttttt tcgctttagc gactttctca aacaccggcg actcatgcag
    34981 cgacacgcga acatacaggc cgataatgac cagcaccgcc gagaagataa atggcacacg
    35041 ccagccccag ctcataaact gctcatcggt cagtagccag gaaagcagca agaaagtgcc
    35101 gttggcaaag aagaagccaa tcggtgcgcc cagctgtggg aaggagccat acagtgcacg
    35161 tttgcgcggt ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt caccgcctaa
    35221 gcccagaccc tgaccaaatc gagccagcgc cagcagcagt ggggcgaaaa taccaatcgt
    35281 ggcatagccc ggcagcagac caatcaccac ggtcgaaatc cccatcgtta gcaacgaggc
    35341 gaccagcgtc gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg cagagccaat
    35401 ggggcgcgcg acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg
    35461 atcgccctgc gggaagaaga tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta
    35521 aaagtcgaag aactcaatgg ccgtgccaat gagagaggcg acaaggactt tattacgcga
    35581 gttgatcggc gtgtattctt gctcgtggtc gagtgttgtg gctgttgctt gcataatctt
    35641 ttcttatttt ttggctaacg aatagccata ttacgcacag caaaagtgat atttcaatgt
    35701 gtaacaaatc gcggaaatgg tcaaaaacca ggcaaaaagg ggataatgtt taggccagtg
    35761 atttttcttc tatgaaatgc ttcacataat tttataaata gtggataaaa ctggtttcag
    35821 gttaaataaa agttaccaac tggatttata tgctggggtt atgagttgga atgaaattgt
    35881 ttgctcccaa atagatggct gaagaggctc tggggaagag tttcaagagg ttatgtgcgc
    35941 attatagccg gatgcgatgc tggcgcatct tatccggcct acggggagcc aggccgaata
    36001 aaatcgtatg ccggataagg cgtttacgcc gcatccggcc tattttaagg ctgcgttttc
    36061 cctggcattc gcgggtcgtc tttatattcg gcggtggcaa tccacgccgc gcaaaacagc
    36121 gtcaaacggg cgaagaagta gaaaaatgcc atcagcccca gcacggaacc aaatgctgcg
    36181 ccagaggggg atttcatcag cgatggcaag gtgtaggtca tcacgatttt aatcacttca
    36241 aaaccaatag cagccagaaa tgttccgcga atcagggctt ttttgcgcgg acggtggcgc
    36301 ggcaaccgcc agaagatcca gaagaaaagc agatagttgg cgaagatgga aatcgccagg
    36361 ccaatcaacc gccacgtcgg tttcagccac tcaatgctgt tcaggtgcag ggcgctaata
    36421 atcatttgct gcgccgaacc ggcaaccgag gtgatcgaaa gcgtcacaat cagcgcaatc
    36481 aacaaaccaa tcagcgaaat aaaatcacgc agatatttta cccagaactt ctcctgatct
    36541 tgcggcgagc gttcccagac atcgcgcgac tgggcacgaa tcgcttcacg caggttaccc
    36601 atccagttga tgccggaata aagcgccacc gccaggccga caagccctac agtcgtacgc
    36661 tgctgaacgg cggtgttgat ggtgtttttc aacgtggcgg ctagcgtcgg atcgctgatg
    36721 ttttgcagaa ttttgtcgaa gatatcctgt agcaacatcg gatgggaggc cagcacaaag
    36781 ccccctgcgg caaacgacac catcaaaatc gggatcatcg ataaaaatga gaaataggtg
    36841 atagccgcac caaactggtt acccagccga tcgttaaagc gttctgtcgc gcgaatcagg
    36901 tgcgcaataa ccggttggcg ttggactttt tcggcagtgg tggtgacggt ttccagcgcc
    36961 tggctaactt tgccgccttt ctcgctatta tccaactgct taatcggctc gtgctccaga
    37021 tcctgggtgg gacgtttgat ctcgttttcc tgcgtcatcc gctcttttgt ccttctgtcg
    37081 tggtcattct taaaaagtat agtcggtcag tccaggtgct ctttcattac gccagccagc
    37141 cactgcataa acaggtttac acgccgggaa agctcccgac gctgcgggta aaccagcgaa
    37201 agggagagcg gctcggcacg gtagcctggc aacacttcaa taagccgccc ggcacgcagg
    37261 gcttcgcgca cggcaatgcg cgggatctga ataatcccca gaccggcaag accggcggcg
    37321 agatagtttt cgctactgtt taccgtcaac atgccgccag acttaaacca ctggacgcca
    37381 ttaacgctgg caacctcaaa acctaacgga tgtacaccca ggtgcggtgt ataacgcact
    37441 attgcgtgtg aagtcagatc atcggggctt tgcggataac caaagcgcgt cagatagtgc
    37501 ggactggcac agttgaccat ggtcagtttg ccgaggggac gggcgataac gccatcttcc
    37561 ggtaacgcgc cagtgcgtat cacgcaatca aaaccatcat gaagaatatc taccggacgg
    37621 tcatggctac tcagttccag ctcaattccc ggatgcagat agagaaattc cgacaggcgc
    37681 ggcagtaaca ggcttttcgc gattcctggc gggatgtcga tgcgtaattt accgctgata
    37741 ctggtggcat cctgttgaaa cagaccgtcc agttcgctga gattactcaa cacatctttt
    37801 gctcgttgat aataggtcat gccttctggc gtgagtttga cccgtcgcgt ggtgcgctgg
    37861 agaagctggg tgccaagttg atgttccagt gcctgtatct ggcgcgaaac acttcctttt
    37921 ggcaaagcaa agaaatccgc cgcgcgggaa aaactttcca gctccgcgac tttgatgaac
    37981 aactgcattg cgtgaatttt atccatttct ttgaccgatt gttgttcaca gcgaaacagt
    38041 aacgcgcata tcgcattgtt tattgttttt tctaacatct aatgccgggt ttcacaatgt
    38101 tgtcatgacg aatgtggcga tttctcagca ggaaaagggc ggaatagctt tctgagaaag
    38161 gtgaatcccc ctaaacagag cgtatttttc agcgattccc tttaaagtga atagaggacg
    38221 tgttggctat ttgtgcttat ttttgtctca ttgccgtttg ttatttttgc ttgtggtcgc
    38281 ggagtttcat ttttaattgc atgcgtacta accaccaata taaggaaatc ctcaaaattg
    38341 ttttaaatag tgccctcgac atgtaatttc aggcgtttag ctgtaactat ttatatcggt
    38401 aactattttc tacgcgtcat ctctgtagaa tggccaccgc caaatggcga attcttattt
    38461 tttctcgcgg gtaataccct tccactactt aagtcttttt aacgtattca ggtggagatt
    38521 cagttatgca aatagtcatg tttgacaggc agtcaatatt tattcatgga atgaaaatca
    38581 gtttacagca gcgtattcca ggagtgagta ttcagggggc cagtcaggca gacgagttat
    38641 ggcaaaagct ggaaagttac cctgaagcct tagttatgct cgatggcgat caggatggtg
    38701 agttttgcta ctggttgctg caaaaaaccg tggtgcagtt tcctgaggtt aaggtgttaa
    38761 tcacggcgac ggattgcaat aaacggtggt tacaggaagt tattcatttt aatgtgctgg
    38821 ccattgtgcc tcgtgattca accgtcgaga catttgcgtt ggcggtaaat agtgcggcga
    38881 tggggatgat gtttctgcct ggagactggc ggacgacgcc ggaaaaggac attaaagatc
    38941 tcaaatcgtt aagcgcccgc cagcgggaga ttttaaccat gttagcggcg ggcgaatcaa
    39001 ataaggagat cggcagagcg ctgaatatca gtaccggaac ggtaaaagcg catctggaat
    39061 ccctgtatcg ccgtctggaa gtgaaaaatc gcacccaggc ggcaatgatg ttaaatatct
    39121 cctcctgaag aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt
    39181 atggttcgcc gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat
    39241 cctgcaacgg atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt
    39301 tctcgatcat tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg
    39361 cgatttcatc acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt
    39421 gtaacggtgc ccagccattg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca
    39481 gaatcccgcc aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt
    39541 catggttcgc catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt
    39601 gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt
    39661 ttaccgcatc gcgacgggca ctggccttct ggcggaacag cgcttctgtc tctttctcgc
    39721 ctttcagcgc cgagatgttg gcgatggcgc tctccagttt aaacaggaag gcattcagat
    39781 cgatggggat gaactgggtg gtacgaatgc tcgccagacg accagtatca cgcagccaac
    39841 gggaagagta atcccaacct gaggccgctc ccgcgcgtaa atcgcggtac acctcgttgg
    39901 gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag ccaggattcg tcacgcggcg
    39961 tgtcgcgatc gtcccaataa cggttgagca gcgatccgtc cggcatccgc acaacatggc
    40021 gataggcctg attagggatc aacgattctg caccgtccat ccagaaggca tattccattt
    40081 taaggtggtc gagatagcgg cgcgcaccgc gtacaccatc ttcttcaaac aactccacca
    40141 tcagcgcaaa aaccggtggt tgcgatcggc tcaaataata ggtgcggttg ccgttgggga
    40201 tgtgaccata gttttcgatc atccaggcga agttatcggc catgcatttc agcaaatctt
    40261 cccgaccact ttccgccagc cccagcatgg tgaaatagga atcccaatag taggtttcgc
    40321 taaaacggcc gcccgggaca atatatgact gcggtagcgc cagcagagaa gaccacggaa
    40381 tgtgatcctg tggttcgcgg gttagcaccg gccacagctg gtcgatatgc tctttcaggg
    40441 aattttgcgg gtccgataca tactcgctgg agtagacctc cggcagccag aagtgatttt
    40501 caacaaactt gcgcaagtca aaatcacgat gacggcgcac tttacggtag cggattaaaa
    40561 tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt tttactgtcg gggaaaattt
    40621 tcgccgactg aacatgctcg aacagttcga gatagcgatc ggcaggcgtc agcgcatcag
    40681 aggcgggcag cccttcaatc atctcgggtt ccggttctgc ctcgatcatc tcatccagtt
    40741 ttaattcata cgggtccagc tcatagcaga gatcgacttc gatcatcagt tcgtctggat
    40801 tagggttttg aattttctga ttgagcataa ggatccaacc tccgaaattc gtcgctaatt
    40861 atcgggcttt tctttaaggg tagtcattcg ctccgcctgg tgggcgacaa agcgtgcggt
    40921 ggatcacgtt tattaacctg tcaaaaaata taacgcacta atatccatgg aatttataaa
    40981 aatacggatt tcgtgttgtt tttcattttc agaacattca atcctgctct cgccgcaatc
    41041 atgatatgcc attcctgatc tgggtaatag gcacaggcta tcttattgat agtttatatt
    41101 catgtaattg attgattcct atcaaaaagc gtatctccct gttttttatg atggctttgt
    41161 ctcgcagaga cgtttgcagc attctggaaa attttctggg tcacaataac tcaccaccgt
    41221 gagggaagaa taatgaaaat ggtctcacgt attaccgcga tcggcctggc tggcgtcgcg
    41281 atttgctatt tagggttatc tggttatgtg tggtaccacg ataataaacg cagtaaacag
    41341 gccgatgttc aggcatctgc tgtcagtgaa aataataagg ttttaggctt tctccgcgaa
    41401 aaaggatgcg actattgcca cacaccttcg gcagaattac ccgcctatta ttatattcct
    41461 ggcgcgaaac agttgatgga ttacgacatt aagcttggat ataaatcttt taacctcgag
    41521 gccgtgcgtg cggcactgct ggctgataaa cccgtttcgc aaagcgatct gaataagatt
    41581 gaatgggtga tgcagtatga aactatgcca ccaacgcgtt ataccgcgct acactgggcg
    41641 ggtaaggtga gtgatgaaga gcgggcggaa atactggcct ggattgcaaa acagcgcgcg
    41701 gaatattacg ccagcaatga tactgctccg gagcatcgca atgaaccggt gcagcccatc
    41761 ccgcaaaaac tgcctaccga tgcgcaaaaa gtggcgttgg gctttgcgct gtatcacgat
    41821 ccccgtttat cggctgatag caccatttca tgcgctcatt gccatgcgtt gaatgcgggg
    41881 ggcgtcgatg gcagaaaaac atcgattggt gttggtggcg cagttgggcc gattaacgcg
    41941 ccgacggtat ttaactcagt atttaacgtt gagcagttct gggatggtcg tgcggcaaca
    42001 ttgcaggatc aggcaggtgg accgccgttg aacccgattg aaatggcgtc gaaatcctgg
    42061 gacgaaatta ttgctaagct ggaaaaagat ccgcagctta aagcgcagtt cctcgaagtt
    42121 tatccgcaag gtttcagtgg cgaaaatatt actgatgcca ttgctgaatt tgagaaaaca
    42181 ttaattacgc cggattcccc atttgataaa tggttgcgtg gagatgaaaa tgcgctgacg
    42241 gcgcaacaga aaaaaggcta tcaattattt aaagataata aatgtgcaac ttgtcatggt
    42301 ggtattattc tcggcggacg ttcctttgaa ccgttggggc tgaaaaaaga ctttaacttt
    42361 ggggaaatta cggcggcgga tattggtcgt atgaatgtga ctaaagaaga gcgtgataaa
    42421 ttgcgtcaga aagtacccgg tttacgtaac gttgctttaa cggcaccgta cttccatcgc
    42481 ggtgacgtgc cgacgctgga cggggcggtg aaactgatgc tgcgctatca ggtaggcaaa
    42541 gagctgccgc aggaggatgt ggatgatatc gtagctttcc tgcacagtct gaacggggtg
    42601 tacacgccgt atatgcagga taaacaataa ttaatttgat cgcccgaaca gg
//
LOCUS       DAZFFI010000029        42327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFFI010000029 DAZFFI010000000
VERSION     DAZFFI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.029"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..1268
                     /gene="ybdN"
                     /locus_tag="ACHJ12_001887"
     CDS             48..1268
                     /gene="ybdN"
                     /locus_tag="ACHJ12_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4989681.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1241..1870
                     /gene="ybdM"
                     /locus_tag="ACHJ12_001888"
     CDS             1241..1870
                     /gene="ybdM"
                     /locus_tag="ACHJ12_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4989682.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1871..3031)
                     /gene="ybdL"
                     /locus_tag="ACHJ12_001889"
     CDS             complement(1871..3031)
                     /gene="ybdL"
                     /locus_tag="ACHJ12_001889"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4989683.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3140..4228
                     /gene="hcxA"
                     /locus_tag="ACHJ12_001890"
     CDS             3140..4228
                     /gene="hcxA"
                     /locus_tag="ACHJ12_001890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4989684.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPAAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4238..4435)
                     /gene="ybdD"
                     /locus_tag="ACHJ12_001891"
     CDS             complement(4238..4435)
                     /gene="ybdD"
                     /locus_tag="ACHJ12_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4989685.1"
                     /translation="MFDSLANAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4617..6722)
                     /gene="cstA"
                     /locus_tag="ACHJ12_001892"
     CDS             complement(4617..6722)
                     /gene="cstA"
                     /locus_tag="ACHJ12_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4989686.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVLLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YESMPENVEEIVAQAKGAH"
     gene            complement(6903..7316)
                     /gene="entH"
                     /locus_tag="ACHJ12_001893"
     CDS             complement(6903..7316)
                     /gene="entH"
                     /locus_tag="ACHJ12_001893"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4989687.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7319..8065)
                     /gene="entA"
                     /locus_tag="ACHJ12_001894"
     CDS             complement(7319..8065)
                     /gene="entA"
                     /locus_tag="ACHJ12_001894"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4989688.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8065..8922)
                     /gene="entB"
                     /locus_tag="ACHJ12_001895"
     CDS             complement(8065..8922)
                     /gene="entB"
                     /locus_tag="ACHJ12_001895"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4989689.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8936..10546)
                     /gene="entE"
                     /locus_tag="ACHJ12_001896"
     CDS             complement(8936..10546)
                     /gene="entE"
                     /locus_tag="ACHJ12_001896"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4989690.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10556..11731)
                     /gene="entC"
                     /locus_tag="ACHJ12_001897"
     CDS             complement(10556..11731)
                     /gene="entC"
                     /locus_tag="ACHJ12_001897"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4989691.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12106..13062
                     /gene="fepB"
                     /locus_tag="ACHJ12_001898"
     CDS             12106..13062
                     /gene="fepB"
                     /locus_tag="ACHJ12_001898"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4989692.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13066..14316)
                     /gene="entS"
                     /locus_tag="ACHJ12_001899"
     CDS             complement(13066..14316)
                     /gene="entS"
                     /locus_tag="ACHJ12_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4989693.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14427..15431
                     /gene="fepD"
                     /locus_tag="ACHJ12_001900"
     CDS             14427..15431
                     /gene="fepD"
                     /locus_tag="ACHJ12_001900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4989694.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLTGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15428..16420
                     /gene="fepG"
                     /locus_tag="ACHJ12_001901"
     CDS             15428..16420
                     /gene="fepG"
                     /locus_tag="ACHJ12_001901"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4989695.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16417..17232
                     /gene="fepC"
                     /locus_tag="ACHJ12_001902"
     CDS             16417..17232
                     /gene="fepC"
                     /locus_tag="ACHJ12_001902"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4989696.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17229..18362)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ12_001903"
     CDS             complement(17229..18362)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ12_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4989697.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18578..22459)
                     /gene="entF"
                     /locus_tag="ACHJ12_001904"
     CDS             complement(18578..22459)
                     /gene="entF"
                     /locus_tag="ACHJ12_001904"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4989698.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
     gene            complement(22456..22674)
                     /gene="ybdZ"
                     /locus_tag="ACHJ12_001905"
     CDS             complement(22456..22674)
                     /gene="ybdZ"
                     /locus_tag="ACHJ12_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO4989699.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22677..23879)
                     /gene="fes"
                     /locus_tag="ACHJ12_001906"
     CDS             complement(22677..23879)
                     /gene="fes"
                     /locus_tag="ACHJ12_001906"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO4989700.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQS
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24122..26362
                     /gene="fepA"
                     /locus_tag="ACHJ12_001907"
                     /pseudo
     CDS             24122..26362
                     /gene="fepA"
                     /locus_tag="ACHJ12_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            26402..26500
                     /locus_tag="ACHJ12_001908"
                     /pseudo
     CDS             26402..26500
                     /locus_tag="ACHJ12_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26537..27157
                     /gene="entD"
                     /locus_tag="ACHJ12_001909"
     CDS             26537..27157
                     /gene="entD"
                     /locus_tag="ACHJ12_001909"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4989701.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27279..27431)
                     /gene="hokE"
                     /locus_tag="ACHJ12_001910"
     CDS             complement(27279..27431)
                     /gene="hokE"
                     /locus_tag="ACHJ12_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4989702.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27782..27934)
                     /gene="hokE"
                     /locus_tag="ACHJ12_001911"
     CDS             complement(27782..27934)
                     /gene="hokE"
                     /locus_tag="ACHJ12_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4989703.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            27903..28172
                     /locus_tag="ACHJ12_001912"
     CDS             27903..28172
                     /locus_tag="ACHJ12_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989704.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28376..29494
                     /gene="ybdK"
                     /locus_tag="ACHJ12_001913"
     CDS             28376..29494
                     /gene="ybdK"
                     /locus_tag="ACHJ12_001913"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4989705.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29560..29808
                     /gene="ybdJ"
                     /locus_tag="ACHJ12_001914"
     CDS             29560..29808
                     /gene="ybdJ"
                     /locus_tag="ACHJ12_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4989706.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29873..30241
                     /gene="ybdF"
                     /locus_tag="ACHJ12_001915"
     CDS             29873..30241
                     /gene="ybdF"
                     /locus_tag="ACHJ12_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4989707.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30335..30988
                     /gene="nfsB"
                     /locus_tag="ACHJ12_001916"
     CDS             30335..30988
                     /gene="nfsB"
                     /locus_tag="ACHJ12_001916"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4989708.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31096..32343
                     /gene="ybdG"
                     /locus_tag="ACHJ12_001917"
     CDS             31096..32343
                     /gene="ybdG"
                     /locus_tag="ACHJ12_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4989709.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32411..33787)
                     /gene="pheP"
                     /locus_tag="ACHJ12_001918"
     CDS             complement(32411..33787)
                     /gene="pheP"
                     /locus_tag="ACHJ12_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4989710.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33889..37032)
                     /gene="cusA"
                     /locus_tag="ACHJ12_001919"
     CDS             complement(33889..37032)
                     /gene="cusA"
                     /locus_tag="ACHJ12_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4989711.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37044..38267)
                     /gene="cusB"
                     /locus_tag="ACHJ12_001920"
     CDS             complement(37044..38267)
                     /gene="cusB"
                     /locus_tag="ACHJ12_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4989712.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38283..38615)
                     /gene="cusF"
                     /locus_tag="ACHJ12_001921"
     CDS             complement(38283..38615)
                     /gene="cusF"
                     /locus_tag="ACHJ12_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4989713.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38639..40021)
                     /gene="cusC"
                     /locus_tag="ACHJ12_001922"
     CDS             complement(38639..40021)
                     /gene="cusC"
                     /locus_tag="ACHJ12_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4989714.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40178..40861
                     /gene="cusR"
                     /locus_tag="ACHJ12_001923"
     CDS             40178..40861
                     /gene="cusR"
                     /locus_tag="ACHJ12_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4989715.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40851..42299
                     /gene="cusS"
                     /locus_tag="ACHJ12_001924"
     CDS             40851..42299
                     /gene="cusS"
                     /locus_tag="ACHJ12_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4989716.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLTQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10079 a        11749 c        10854 g         9645 t
ORIGIN      
        1 agacttatct cactgatcac cctgtaacgt tcagagagcg tcttcagatg tctatttata
       61 aaattcctct tccgctcaat attctggaag ctgcacgaga gcgtattacc tggacactca
      121 acaccctacc ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc
      181 atctgacagc cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact
      241 gggaggcgca attctcttgc accattaact atgttcagtc cctgcgcgag ttgtacaccg
      301 atgtcatcga agagttttac tgggttgcgc tcccgcttac gacgcaaaat tccctttcac
      361 aataccaacc cgaatggcag tgctgggaac ctgatgtcga atgggtacgt caacctcccc
      421 aagatgcgat aaccgaccct gactttttct ccttttacca gccaggcatg accttcgaac
      481 aatttgtacg tgagtttgcc gaatggtttt cacaaaaacg tccggcggcg atgatgatcg
      541 gcatccgtgc ggatgagtcc tacaaccgtt ttgtcgccat cgccagttta aataaacaac
      601 gttttgccga cgataaaccc tggactacgg ccgcaccagg cggtcatagc tggtacattt
      661 accccattta cgactggaaa gtggctgata tctggacctg gtatgcaaat catcaaagtc
      721 tctgcaaccc actgtataac ctaatgtatc aggcaggcgt tcctctgcgt catatgcgaa
      781 tttgcgaacc ttttggcccg gagcaacgac aaggattatg gctctatcac gttatcgaac
      841 cggatcgctg ggctgctatg tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg
      901 ccggacatga taatcatttc tatgggcatc ggaaaatcct caagccagaa catttagact
      961 ggcaagaata tgcattattg ctgctcaata gcatgccgga aaaaacagct gagcattacc
     1021 gcaataaaat cgccatttat ttgcactggt atcagaaaaa aggcatcgag gttccacaaa
     1081 cccagcaagg ggacattggc gcgaaagata tcccctcctg gcggcggata tgcaaagttt
     1141 tactcaataa cgattactgg tgtcgggcat tatcatttag tcctacgaaa gcgaagaact
     1201 atcagcgtta taacgaacgg ataaaaggaa aacgtcagga atgggggata ctatgcaaca
     1261 acgattaacg caggatttaa cgcaatttct cgccagcttg ccggaagatg atcgcatcaa
     1321 agcgattaat gaaatccgca tggcgatcca tcaggttagc ccctttcgtg aagagccagt
     1381 ggattgcgtt ctgtgggtta aaaacagcca acttatgcct aatgattaca acccaaacaa
     1441 cgtggcacca cccgaaaaaa aactgctgaa aaaatccatt gaaatagatg gttttaccca
     1501 acccattgtg gtcacgcata cggataaaaa tgccctggaa atcgtggatg gttttcaccg
     1561 ccatgaaatt ggtaaaggct catcatcatt aaaattacgc ttaaaaggtt atttgccggt
     1621 gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg actattcgtc ataatcgcgc
     1681 tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc cgtgaactta gccagttggg
     1741 atgggacgat aataaaattg gcaaagagct aggaatggac agcgacgaag tgctgcgcct
     1801 gaagcaaatt aacggcctgc aagagctgtt tgccgaccgt caatattccc gcgcctggac
     1861 ggttaaataa ctaaagctgg cgcaggcgtt cagctgctgc cagcaacgtc gattccttct
     1921 tggcaaaaca gagacgaatc agtttatgtg ggaagggatc ggcgcaaaac accgacagcg
     1981 gaatcgccgc tacgccgtgc tcctgcgtca gccactggca aaactcaaca tcatccaggg
     2041 tagaaacctc gctgtaatcc accagcaaaa agtatgtacc ttcacacggt aaaatctcca
     2101 gccggctttc atttaaagca ttcaccagaa tatcgcgctt ctggcgataa aagtccggta
     2161 acgcaagata atgctcaggt tctgcacgta gcatatcagc aagcgccagc tgtgccgggg
     2221 tattcaccga aaaggtcaga tactgatgta ccttgcgaat ttcggcgctg atgggcgctg
     2281 gcgcaacaca ataacccact ttccagccgg tcatatgata ggtcttgcca aatgaagaaa
     2341 ccgccactgc ccgctcacgc agctgcggat gcgccagcac actggcatgg ccctgttgtg
     2401 aaaagttgat gtgctcgtag acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg
     2461 cctgccacaa agcggcgaaa tcagcctgct gccagacagt tgcactgggg ttatgcggag
     2521 tgttgaggat caccagtctg gtgcgctcgc ttaataatgc ggcaaattcc tgccagtcaa
     2581 cgcgaaaatg cggtggttgc agtgccatac gcttcactat tcccccagaa agcgcgatgg
     2641 cgggggagta actgtcatag ctgggatcaa aacaaatcac ttcatcgcca ttgcgcacca
     2701 gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc cgttacggtg atatcgctat
     2761 cggcatctgg ttgatagcca tacaaacgtt ccgttttctg agcaatcgcc tcgcgcaagg
     2821 cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc
     2881 gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc ttgcgacagg ttaatcgcct
     2941 ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt
     3001 tgctttgtgg aatcagaggg ttatttgtca tttttattgt acctgacgaa gttgcgtgtt
     3061 gaaggcacta taacacgatg ctagtatttg gcaatcaaga cgtttagatg tctaaataaa
     3121 acaataagga caacacaaca tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa
     3181 ctacttttca catccaggaa gtttcaatca cctgcacgat tttttcactg atgaacaact
     3241 ttctcgcgcg gtgtggatct acggcaaacg cgccattgct gcggcgcaaa ccaaacttcc
     3301 gccagcgttt ggacttccag cggcaaagca tattttgttt cgtggtcatt gcagcgaaag
     3361 cgatgtacag caactggcgg ctgagtccgg tgacgatcgc agcgtggtga ttggcgtcgg
     3421 cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc cgtctcggtc tgccatttgt
     3481 tgccgctccg acgatcgccg ccacctgcgc agcctggaca ccgctctccg tctggtataa
     3541 cgatgccgga caggcgctgc attatgagat tttcgacgac gccaatttta tggtgctggt
     3601 ggaaccggag attatcctca atgcaccgca acaatatctg ctggcgggga tcggtgacac
     3661 gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac
     3721 cgtgcgactg gggatcaata atgcgcaggc cattcgcgac gtattgttaa acagtagcga
     3781 acaggcgctg agcgatcagc aaaatcaaca gttaacgcaa tcattttgcg atgtggtgga
     3841 tgctattatt gctggtggtg ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc
     3901 ggcggctcat gccgtgcata acggtctgac tgtcctgccg caaaccgaga aatttctcca
     3961 cggcaccaaa gtcgcttatg gcattctggt acaaagcgcc ttgctgggtc aggatgatgt
     4021 gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg ccgaccacgc tggcggagct
     4081 ggaagtggat atcaataatc atgcggagat cgacaaagtg attacccaca ccctgcgtcc
     4141 ggtggaatcc attcattacc tgccagtcac gctgacacca gatacgttgc gtgcagcgtt
     4201 cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta gcagcagcgc gcgccgcctt
     4261 ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc ttcgtaggtc atcggcgttt
     4321 gatcgggatg gttaacccgc atatgttcga catagttgtc gtaatcaggc ataccaatca
     4381 tcagcttcgc cgcctgacct aaatattttc cggcgtttgc cagtgaatca aacatcttgg
     4441 cctccgctaa ccatgttgct gaaagagtgc cggatgcggc gtgaacgcct tatccggcct
     4501 acaaatgcta tcaacgcgct cggtcagtcc cctcgcccca tcggggagag ggttagggtg
     4561 aggaggaacc ctcggcacta tgccaacatt cgccaacatc ccccctcact ctgactttag
     4621 tgtgcgcctt ttgcctgcgc cacgatctcc tcgacatttt ccggcattga ctcatacggc
     4681 gtttctttcg ccgttggctt cggatctttc aatgccgcca gtgccgtctt aatcgagaac
     4741 agcgccagaa ccaccacgac caccataaag aagatggtta acccggcatc cagacggttg
     4801 ttgaacacca gttgcgccag ctgtgactca gtatactgcg acggaatatt gccgctgtcg
     4861 atcattgcct ggaacttatt agcaatggcc aggaagccga ctttcgcatc cgggctaaac
     4921 gctttctgcc agcctgcggt cagggtacaa atcagcagcc aggccgttgg taccagcgcc
     4981 acccaggcgt aacgttgacg tttcatcttg aacaacacca cggcacagag catcagcgcc
     5041 atccctgcca gcatctggtt ggcaatacca aacagcggcc acagagtgtt aatgccgccc
     5101 agcggatcga ccacgccctg atggaggaag tagccccacg ccagtacgca cagcgctgtt
     5161 gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc ccggagaaac cacgcccagc
     5221 agatcctgca acataaagcg cgcagcacgc gtacccgcat ctaccgccgt cagaataaac
     5281 agagcttcaa acagaatggc gaagtgatac cagaacgcca catccatcat accacccagc
     5341 gcaccgtgca ggatgtaagc catccccacc gccagcgtcg gcgcaccgcc cgcacgggaa
     5401 atgatcgact gttcacccac ttcgctggca atctggttga gcgtatctgg tgtaatgcta
     5461 aagccccagc tactcaccac ctgcgcggca gaagcgacta catccgccgt ccctgccgga
     5521 gccagcaccg ccattgggct gttcatggca aaatacacgc ccggatcgat gatacaggca
     5581 gaaaccagcg ccataatcgc cacgaaggat tccatcaaca tcccaccgta gccgataaag
     5641 cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc cggaagagat cagcgcatgg
     5701 aagccagaca ccgcaccaca ggcgatggtg ataaacagga acgggaacag gttaccggtc
     5761 cataccgggc cagtgccatc gacaaatttg gtcagcgcag gcatggtcag cgtcgggcgc
     5821 ataatcaaaa tgcctaccgc cagaccgacg atagtcccga ttttcaggaa ggtcgagaga
     5881 tagtcacgcg gggccagcag caaccacacc ggcagcaccg ccgccacaaa accgtagccc
     5941 accagcatcc aggtcagctg cacgccggta aagtcaaagt acggtgccca ggtcggactt
     6001 tctgccaccc agccgccaga gataatggcg aaaatcagga gcaccaggcc aatcactgac
     6061 acttcgccaa tgcggcccgg acgcagatag cgcaggtaga ttcccataaa aatcgccagc
     6121 ggaatggtga acgcaacggt atacgttccc cacgggctat gagtcagggc tttcaccacg
     6181 atcatcgcca gtactgcaag gataatgacc atgatcataa agcaggctac cagcgcaatc
     6241 accccggcag ttggccccat ctcttctttg accagctcac ccagcgatcg accgtcacgg
     6301 cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac cggcgagaac caccccggcg
     6361 agcagccaga tcatccctgg caggtaaccc atttgcgccg ccagtaccgg cccaaccaac
     6421 ggacctgctc cggcaatggc cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc
     6481 acatagtcca gcccgtcgtt atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc
     6541 agcacatttt tggcgatata cagcccataa aaacggtaag cgatcagata gatacagacc
     6601 gacgccacca caatccacag cgcgttgatc tgttccccac gatttaaagc aatgtatccc
     6661 agagcaaatg ctcccattac agagagcact gtccagacga ggtatttccc tgatttgttc
     6721 atagttgtta tccgtgtgcg tgtccataga gatgttacat tttgtttcta taacatcttt
     6781 gtacgattta acaacacggt aaccatgtaa atcggagttg aaaccaatat ttaacttaac
     6841 aatgttaact cgatcactcc gttaaccgag ttttcctgca atctcattta attctgtcgg
     6901 gctcatccca aaactgccgt acccagccga caagtgcagc aacgccgccc ctgttcatcg
     6961 aaaacgacga tttcccagct ctgattttgc cgcccaagat gcagcggctg gcagacgccg
     7021 cgtaccttgc cttcagacac cgggcgatga tgggtggcgt taagttccgt acccaccaca
     7081 cactgcccgt cacgggtcat cataaatccg gccatcgatc ccagcgtttc agccagcgcc
     7141 gctgacgcgc cgccatgcag caggccaaac ggctgatgag tacgggtatc aaccggcatt
     7201 tcggcttcca gcacatcatc tcccagacgg gtatacacaa ttcccagatg cgccaccatt
     7261 gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt
     7321 atgcccccag cgttgagccg ccatcgacca caatatcctg tagggtgata tggctggcga
     7381 ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg tggacgggcg attttcccca
     7441 gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat acgctgttct tcggcgtcat
     7501 cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga gccaggcgaa accacattac
     7561 agcgcacacc gctaaccgcc agttccagcc cgacgctcaa cgccaggctt ttcagcgccg
     7621 ctttcgatgc gccataagca ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca
     7681 cagtgacaat cgccccgccc cgctgacggc gaaactggtt catggtttgc tgaaacaggt
     7741 taaacgcacc gccgacgtta accgcaaaag tctgctgcca gtcctctttg ctcagttggt
     7801 ctgtcgcgcc catgcgtaaa attcccgccg cattgaccag cgcgtccagt cgctccgttt
     7861 cagctaacag tcgctgacac acttgcgcca cctgcgcagc gtcggcaaca tccatcactt
     7921 cggtcgcaaa gggatattgc tcctgagtga acgcttgatc aaaacctgta actttcgctc
     7981 ccgcctcaac aaacgccagc gccgtggcgt agccgatacc tttacctgcg ccggttaccc
     8041 agacattttt accgctgaaa tccattattt cacctcgcgg gagagtagct tccaccaggc
     8101 gtcgatggtc gggtttttcg ccagcatgac aaaatcgata tcaccatgca ctttgcgcca
     8161 gcgcgccgcc agcgccatca tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc
     8221 atcgaacggt tcatcggact cgtccagcaa cggcaggatc acctcacgca gcgccgcttt
     8281 gctggcgggg acaggtgctg gcagcaattc ttcggtcatc accacccggc cagaacgtcc
     8341 ggccacatat ttcagcgaca tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc
     8401 caccataaac ggtttaatat cgcgcataaa tgcgtcggtt gcggtagtca tacagccaat
     8461 gtgggcatat accccggtaa taatcagctg gttacgtcca ctctctttga gcatttgctc
     8521 cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc
     8581 tggcgtcagg cgatccacca ccttttgctg ttccggcgaa cgggtcaggc ccggccccca
     8641 catatcattc aacagcgccc gatcttcatc gctctgctct ttcggctggg cggtgtaata
     8701 aaccggaata ttgtgctgtt tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg
     8761 ctccatcatc gggcagttct cgccccagaa gctgacaaaa tagtcctgca tatcatggat
     8821 taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact ttattcttag ggatatcgtg
     8881 agactccggc agtgcgtaag cctgtaattt tggaatagcc atcgtgttct ctccttcagg
     8941 ctgatgcgcg tgacgccagc cactgacgta attgtttttt atcgactttc ccgaccgccg
     9001 tcagcggaag tgaatctaca cactccacgc gatccggtaa tttaaattcg gcaataccct
     9061 gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg ctcttttacc accagataag
     9121 cgcagctttt ttcgcccatc agctcatctt ccatgctcac cagtgcggcg tagatcaccg
     9181 ccgggtggcg cagcagcagg ttttcgatct cttcggcagc gatcttctca ccgccacggt
     9241 tgatctgatc tttctcgcgc ccctgcacgg tgatgtaccc ctctggatca atagagatca
     9301 gatcgccgga acagtaaaaa ccgttggcat caaaggcgct ggcattgtgc tgcgggcttt
     9361 tgtaatagcc acggaaggtg tacggaccgc gggtcatcag gcgtccaact tccccttgcg
     9421 gcagtggatt tccttcggca tcggcaaccc acacttcatc gtccggacac attgggtaac
     9481 cctgggtatg gataattttc tccgcgctat catcaagacg ggtgtagttc accagccctt
     9541 ccgccatgcc aaacacctgc tgcaactggc agccaatctc agcgggaata cgcgccgcaa
     9601 gggtggcaga aagacgtgcg ccgccgacct gtaacagttt cagcgaggca agctgcgccc
     9661 ggctttcacc ttcggtcagc gcctgcaacc acaggctgac tgccggtggc accagcgcgg
     9721 tgacgttcac ctgatgtttt tcaatcaatg ggaagcaaag cgtggcgctg ggatcggccg
     9781 ccagaacaac cgttcctccg gcaagaaaga cgcccagcga tcccggcgaa ctcatggcgt
     9841 agttatgagc cgccgggatc gcgcacaggt agcgtgtctg ttgtgtgaac tgacaaatct
     9901 cgacgctacg acgcacgctg tagtagtagt cgttatgagt gcgcgggatc agtttcggtg
     9961 tgccggtggt gccgccggaa agctggaaat aggccacttc atcagcaggt gatggcgtgg
    10021 cagtaaaatc ctcagccgga tggttaatcg catcctgcaa gttatgctca ccgctgtcgt
    10081 tgtgcagttg caccacgcga atggaggaat gttctgtgac gaaagtattg aggaaatcat
    10141 ccccgctaaa cagcgcatgt tggcgatcgg caatcagcaa tgcgggttca atctggctgg
    10201 cataggcgtt cagttcacta cgctgatggc tgaacagcgc cagcaccggc gcaacgccca
    10261 gtttcagcag cgcgaaaaag gtaatataca attcagcgac gttacccagt tgtaccagcg
    10321 cggtttcacc aggtttaatg ccctgacggc gtaaactaca cgcgaggtta tccgccgcct
    10381 gattcagctc ccggtaactc aactgtcgct cgccgtcgat aaccgcgatg ctgtcactcg
    10441 cagcgtggcg agtcagaatg tcggtcagcg gcaaatcctg ccagtagcct ttttcccgat
    10501 agcgacgggc aaactcttcc ggccagcggg tgaatggaat gctcatcctc gctccttaat
    10561 gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca
    10621 acggtgacga cgcaggcaca atccccgctc cggcaaacag acgcacctga ttttcccgca
    10681 gcttcgcgca gcggatggtc accacccatt cgccgttacc ttcgctgtca caccaaccca
    10741 caatgccgcc aaacagttcg cggtcgaatg gctccagttc agcaataacc tgggtcgcgg
    10801 cctgatgcgg gaaaccgctc agcgcggggg ttggatgcag cagacaggcc agagtcagtg
    10861 cgttttcttg cgaattcgct ttaccttcaa agggagttgc gagatgccac agcgtcggcg
    10921 tggtaatcaa ttgtggagag gagggaacgt gtaactcact actgcgttcg cgcagtacct
    10981 ctttcatcgc ctgagtcacc agttcatgtt catggcgatc tttttctgac gccagcagac
    11041 gattacccgc ttcgcgatcg agcacttcat ccggctgacg acgcgcggaa ccggctaacg
    11101 gaatggagct aaaacgctcg ccgtctttac gtagcagcag ttccgggctg gcccccagca
    11161 ggacgccacc atcagccagc gggacatgga agttgtaact aaccgggttt tgcgcaatca
    11221 accgttccag caatacgcca ctatcaatgg cggcgtcagt ggtgatatca atcaatcgtg
    11281 acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc ggcggcgcgg gcaaccatct
    11341 gttcaaacgt ggtttgttca ggaattgcct ggcgttccac cacgttcagc gactggctgc
    11401 gggtaaaacg gcgggctgag gtctgttttt cctggcggga gaacgactgc caggattcgg
    11461 gaatatacag cgacgaaggc tgacgtggat cgaagggaat cgccccgacc atcaccggat
    11521 ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc gagtttttgc tggaagggac
    11581 tgtcgggcga atccccgttc acagccggtt catcgaagcg ggcgaaacat cctgacgtcg
    11641 taaaactgcg gtacggcgac ataaagaaaa agcgattggg cgcaagtgtt gccatggtct
    11701 gctgtacttc ctcagccagt gacgtatcca tatcatcctc cacaaaatga taaaggcttt
    11761 aataatgatt atcatttata ttttcggtcg ctaacctaaa agcaaacgcg cactatgtca
    11821 actcttgagg taacgcaatc tgcaactcgg ggttgcatct ggttgtggtt acaatgaaaa
    11881 tgagaagcat tattgatgga ttcgcataag cgcaatgtga tgtcctgcgc cgttctgccc
    11941 cctctccctt ccagggtgag ggctggggtg agggttaatg ttcgcaccag tgctggctgt
    12001 tcccctcacc ctaaccctct ccccaaaggg gcgaggggac ggattgtgcg ctttgtcgaa
    12061 tttgtcatta cgcccttaac cttattaata acaggaagct gatttgtgag actcgccccg
    12121 ctctaccgca acgcccttct attaacagga cttttacttt caggaatagc cgcagttcag
    12181 gccgctgact ggccgcgtca gattactgac agccgtggca cacatacact ggaaagccag
    12241 ccgcagcgta ttgtttccac cagcgtcacc ctgaccggct cactgctggc gattgatgct
    12301 ccggtgatcg ccagcggcgc gaccacgccg aataaccgcg tcgcggatga ccagggcttt
    12361 ttacgccagt ggagcaaggt ggcgaaagaa cgcaaactgc aacggctcta tatcggcgaa
    12421 ccgagcgccg aagccgttgc cgcgcaaatg ccggatctga ttttaattag cgcaaccggc
    12481 ggggattcgg cgctggcact gtatgatcag ctttccacca tcgccccgac attaatcatc
    12541 aattacgacg acaaaagctg gcagtcgctg ttaacgcagc ttggcgaaat taccgggcat
    12601 gagaaacaag cggcagagcg gattgcacag tttgataagc aactggcagc ggcgaaagag
    12661 caaatcaaat taccgccgca gccggtcact gccattgtct ataccgccgc tgcacacagt
    12721 gccaatctct ggacgccgga atcagcacaa gggcagatgc tggaacaact cggctttacg
    12781 ctggcgaagt tgcccgcagg gttaaacgcc agccaaagcc agggcaaacg ccatgacatc
    12841 attcagcttg gtggggaaaa tctggctgct gggttaaatg gcgagtcact attcctgttc
    12901 gccggtgatc agaaagatgc cgatgcgatt tatgccaatc cactgctcgc gcacctgccc
    12961 gcagtacaaa acaagcaggt ttatgcgctg ggaaccgaga cgttccgcct ggattactac
    13021 agcgccatgc aagtgctgga acgacttaag gcgctgtttt aagcattaac tgtcagacgc
    13081 tgtcacctgc ggcggcgtct ggcgaaaacg tcgcaactcc accagcacca gcaataacaa
    13141 cacgccgata atcaacaaac caaaaccgct cgcgcttgcg gaagcaaccg gtgtcatcat
    13201 cgcgcccaaa ccacccagca gcgccgcgcc tatggcatcg cccgtcacgt tctgcgccgt
    13261 ccacaaaccg ttaatccgcc ctaacatcgc ttccggggtt tgcgtttgca gcattgtata
    13321 ttgcagcaac gaactaaccg cgctcagcca gccgaacagc gccagacaaa ccacgcctaa
    13381 aatccacatc ggcatcaggc caaacagacc aatggcgagg aacgatccca gcgtggagag
    13441 cagcatcaat aaccctggtc gcgcactatg tgccagcttc ccgctggtta acgcaccaat
    13501 agccgcgccg agcgggatcg ccgcgtagag aaaaccaatc tgtgccgctg acatctgcca
    13561 gttgtcagcc agcgccggat acagtacccg caccgcgctc gccatcgtca ataaaccacc
    13621 cagcagcgca atcccgccca ccagcgggct ggcaagcaga aaacgaaatc ctgccagtaa
    13681 tgatttcaac ggatgctcac gcggctgcgg tggcggtggc aacgccggaa ggcttaacaa
    13741 cggtagcaag gtaataaacg tgcccgccgc cgccagcccg tagttccagg ctacgccacc
    13801 ggtcgctagc aataaaccgc caatcatggg cgaaatcacc gaccccagac gcacggtcaa
    13861 catggtgatc gccccggcct gcattaagtt ttcacgccct accagtgctg gtgtcgccgc
    13921 caatagcgcc gtaacgccaa gcgatgcgaa aaaaccatcc cataaaccaa gtaaatagat
    13981 tgccagcaat gacggctccg gcagcagtgc attaaggcac agtccaatga agccaatgcc
    14041 acaggtgccg cgcgccagca aaatcacttt tttgcgctca tagcgatccg ccagcacacc
    14101 gccaaccatc aggccaacaa acatcgcgcc gccggtcagc gtcaccgaaa gccccacctg
    14161 ccaggtagaa tgcgtcatca tctggatctg caccggcacc gcgacgccga gcaaacccag
    14221 agacacaatt gagataaaac gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa
    14281 caggctgagg ttaagcagcc aggattgttt attcattaca atgccttgcc atcaaatatt
    14341 ttaaatacct attcttaggc tgcacatgct aacatatcca aataagatcg ataacgataa
    14401 ttaatttcat tatcatggaa gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg
    14461 ccgtgcccgg attgctgtta ttactgatta tcgcgacggc attaagcctg ctcattgggg
    14521 caaaatcact ccccgcttcc gtagtgctgg aggccctctc cggcacctgc cagagcgccg
    14581 actgcaccat cgtgctcgac gcgcgactgc cgcgcaccct taccggttta cttgcaggcg
    14641 gcgcgcttgg ccttgccggg gcgttgatgc aaaccctcac ccgaaatcca cttgccgacc
    14701 ccggcttgct tggcgtgaat gccggagcca gctttgccat tgtgctgggt gcggcgctgt
    14761 ttggttactc atccgcgcag gaacaactgg cgatggcctt cgccggggcg ctggtggcct
    14821 cattgattgt tgcctttacc ggcagtcagg gcggcgggca gttaagtccg gtgcgtttaa
    14881 ccctggcggg cgtggcgctg gcggcggtgc tggaaggact gaccagcggc atcgccctgc
    14941 ttaatcctga cgtctacgat caactgcgtt tctggcaagc cggttcgctg gatattcgca
    15001 atctacatac cttaaaagtg gtgctgatcc cggtgctgat cgccggagca actgcgctat
    15061 tactgagtcg cgcgctgaac agtttgagcc tcggcagtga caccgcgacg gcgctgggca
    15121 gtcgcgtggc gcgcacacag ttaattggtc tgctggcgat taccgtgctt tgtggtagtg
    15181 cgacagcggt agttggcccg attgccttta ttggcctgat gatgccgcat atggcgcgtt
    15241 ggctggtggg tgccgatcat cgctggtcgc tgcccgtcac gctactcgcc acccctgccc
    15301 tgctgctgtt tgccgatatc atcgggcggg tgattgtgcc cggcgagttg cgcgtttctg
    15361 tggtcagtgc gtttattggt gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag
    15421 gtggtgcatg atttacgtct ctcgccgatt actcatcacc tgtttgctgc ttatttccgc
    15481 ctgtgtggtt gcaggtatct ggggattacg cagcggtgcc gtcacgctgg aaacctcgca
    15541 ggtattcgcc gcgctgatgg gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg
    15601 gcgtttacca cgcgtgctga tggcgctgtt gattggcgcg gcactgggcg tcagcggtgc
    15661 gatttttcag tcgctgatgc gtaacccgct cggcagccct gacgtaatgg gctttaacac
    15721 cggggcgtgg agcggcgtgc tggtggcgat ggtgctgttt ggtcaggacc tgacggctat
    15781 cgcgctggca gcaatggtgg gcggcattgt cacttcgctg ttggtctggc tgctcgcctg
    15841 gcgtaacggc atcgacacct ttcggttgat tattatcggt atcggtgttc gcgccatgct
    15901 ggtggccttt aatacctggc tgttgctgaa agcgtcttta gaaacggcgc taacagcggg
    15961 tttgtggaat gccggatcgc tcaatggcct gacgtgggca aaaacttcgc cttccgcacc
    16021 catcattata ttgatgctca ttgccgccgc cttgctggtg cggcgaatgc gcttgctgga
    16081 gatgggcgat gataccgcct gcgcgctggg cgtcagcgtc gagcgttcgc gtctgttaat
    16141 gatgctggtt gcagtggtgc ttaccgctgc ggcaacggcg cttgccgggc cgatttcctt
    16201 tattgcttta gtcgcgccgc acattgcccg acgcattagc ggcaccgctc gctgggggct
    16261 gacccaggcg gcgctgtgcg gagcgctgtt actgctggcg gccgatctct gcgcccaaca
    16321 actgtttatg ccgtatcaac ttccggttgg cgtcgttacc gtcagcctcg gcggtattta
    16381 ccttatcgtc ttgttaattc aggagtctcg caaaaaatga ccgaatcagt agcccgtttg
    16441 cgcggcgaac agttaaccct gggatatggc aaatataccg ttgcggaaaa tctgactgta
    16501 gaaattcctg atggtcactt cacggcaatt atcggaccaa atggctgtgg taaatccacg
    16561 ttactgcgta ccttaagccg cctgatgacg cctgctcacg ggcatgtctg gctggatggc
    16621 gagcacattc aacattacgc cagtaaagaa gttgcacgcc ggattggtct gttggcgcaa
    16681 aatgctacca cgccgggcga tatcaccgtg caggagctgg tggcgcgtgg acgttatccg
    16741 catcaaccgc tgtttacccg ctggcgcaaa gaggatgaag aagcggtaac gaaagcgatg
    16801 caggccacgg gaataactca tctggcagat caaagcgtgg ataccctttc tggcggacaa
    16861 cgccagcgag cgtggatcgc gatggtgctg gcccaggaaa cggcaattat gctgctcgac
    16921 gaacccacga cctggctgga tatcagtcat cagattgatc tactggaact gttaagcgaa
    16981 ctgaaccgcg agaaaggcta taccctggcg gcggtgctgc acgatcttaa tcaggcctgt
    17041 cgttacgcca gccatttgat tgcattgcgg gaagggaaaa ttgttgctca gggagcgccg
    17101 aaggagattg tcactgctga actgattgag cgcatttatg gtctgcgatg catgatcatt
    17161 gacgatccgg tagccggaac gccgcttgtg gtgccgctcg gacgaacggc accttcaacc
    17221 gcaaatagtt aaactaagtg gtctgccatc atggcatcct gttttctgga tgccatcgca
    17281 tagcgcaata acacgctacc acaagccacc atcccgccga tcagcgcgga aaggatcaca
    17341 ataatcgcct tacccggacc gtcttttttc actggcaatg acgggcttaa ctgatattta
    17401 aacggcgtaa aattcacatc gttgatattt gcttttgtta actgctcaac aagatactga
    17461 cgattacgta attcaccgtt cagttccgca acgtcagtga ccgctttttc aatttccagt
    17521 ttgcgttcaa taccgtctgc gccgagagaa atagagaaat cggggtcatc tttaacggcc
    17581 tgaccattac tgtatacggg ctttttaatt cccgccgcgt tggcaatgtc gagtgaataa
    17641 ttgaggcgct gaatgtttgc atcaagttga tttttcgttt taatgcgatc ctgagccagt
    17701 ttttcttttt caaactgggt tttgatctcc agtttattac ggacgttttc tatcgactct
    17761 ttcaccacca acgcagagat ataatcgata tacccgctca aaacggtctg cgcctcttta
    17821 ctggttggcg cggtaaaact tagcgtccag gaggtataca gtgacggttc atcttttttc
    17881 ttactggcat tgtcatcaac cgctttcatt ttttcgctca atgcaacaat agcgcgatgc
    17941 aaatccagtt catcgatttt cgcctctttt aattggtcca tcacataagg tgatgaacgc
    18001 aggtactctt ccagcaagct aaccgactga aacttcttga taaacaggtt aaatgcaccg
    18061 ccacggtcga tattaacatc gagatcaaga acgcggagtt tggtaaaggt tttctccagg
    18121 tcctgccact gaatggcttc ggcgggagta ataacggctg agctggtcca tttttgcggc
    18181 aggatgaaag agatcagtaa acctgcgcag gcaaatgcaa aaacgaccgc catgaccgtt
    18241 tttttggccc gccataaaac ttcgattaga ttaagtaaat caatttcatt attactgggc
    18301 gacgccagag gatactcaga aaaatgagcg tcacttccct gtttaatatt caatgatgac
    18361 atggggtata acctgaaaat gagtccaata ggggaaaatc tccacggcgc tcactttacc
    18421 agttggtgag aattatccga agctgaagtt tgtaaattcc ttccactgaa tgcaacattc
    18481 actctattta agagaaataa ctagcaaact tataaactaa ataaatgatt aacccataat
    18541 tatttcctat aattatgggt taatagataa tgttaattta cctgtttagc gttgcgcgaa
    18601 taatcggccc aattttttca aacgcctctg aagatataat atccacatgc gcacaatcct
    18661 gacgataaat atccagctcg gctatccacg gcgaccaggc gcgttcggga ctcatacctt
    18721 cctgaagcgt gcgttcagca acaaacagcg tcgctttacc gtcaaacggt acgctatgag
    18781 cagtcgtcag caggcgcact gcatcagcgt agttgccttc aatggtggta aacaactccg
    18841 ttgaagtact tccctgctgt gccgccagga aggcctcgcg ttcgcggtta atctccgcca
    18901 gcacttccgg gtccagacca ttagcctctt tttcctgcca gttttgcgtc tctggcggcc
    18961 aggtatccag caaaccaaga aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa
    19021 taccctgcgc cagcgtaccg ccaagggaat accccagcag gtaataaggg ccgtgcggtt
    19081 gttgctcaag taacgttgcc agatgcgctt cgcagacttc atccaggttt gtcgctgtct
    19141 gcatgggtcc atgagggcgc ggcgactgaa tgccaataat cgaccattgt ggatcgagat
    19201 aacgcgagag cacgctaaac tgccaggcaa aaccggacgc cggatggaaa caaaacagcg
    19261 tcgggccatt accttcacgc aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt
    19321 cctcttcacc atcaataatc gttgccagtt tggcgacggt tgacgcaacc atcacctgcc
    19381 ccggcgtcac ctgacgggca aactgccgac ttaactgcgc tgccagtttc attgccagta
    19441 gcgaatgacc gccaagcgcg aagaaatcag cgtcagcatc ctgcacgtca caccccagca
    19501 acgacgagaa tgccgcggcg ataatcgttt cactgcccgc tttcggcgca cgccctggcg
    19561 cttgtgcctt cagttcaggc aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa
    19621 gtggcaactg cggcagttgc agcagaacga ctggcaccat atgcggcggc aatgtttcac
    19681 gaagctgcgc ctgcaatgcg ctggtatcca acggcaggcc cgattgcgat acaagatagc
    19741 ccaccaactg acgcgcatca ccaccagtgg ctgccgcctg gttaatcaca caggcgtggg
    19801 taacggcttg ttcgacatcc ggcagcgcct gcatcacgcg atcgatttcg cccagttcaa
    19861 tacgctgccc gcgaattttt agctgatcgt cactgcgccc gaggtactcc accgcgccgt
    19921 tatccagcca gcgggcaacg tctccggtac ggtacatccg ttcaccagga gcaaaaggat
    19981 cggcaataaa gcggctggcg gtcagatcgg gtcgtccaag atacccctgc gccagttgaa
    20041 taccggtgag atagagatct cccgccacac ccggcggcac cggatgcatc atcgcatcaa
    20101 gaatacgcag acccgtattc cataccggat aaccaatcgg cacactgctg ccgcgcacct
    20161 gtgccagttc ctcgccaaaa gccggatacc agctgacatc taccgccgct tccgtcgggc
    20221 catacaggtt atgcaacggc gcgccggtta actgttgcca ttcgcggcat aaatcagccg
    20281 gtaaggcctc accactacag aaaacctgtt tcaacgtcgc gcaattctgg cgagcggttt
    20341 gcggcgtcag cgatgcaaca aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta
    20401 cgccatattc ggcaaagaat tgctgcatag cgagcgggtc acggtgcgct tccggttcag
    20461 ccatcaccag ttttgcccct gcgataaacg gccagaaaaa ctcccacacc gagacatcaa
    20521 aactgcacgg cgttttttgg gcaacgacat cttcgcctgt aagcgggtag tgattttgca
    20581 tccataacag gcggttaacg atagccgtct gcccgaccat tacccctttc ggcctgccgg
    20641 tggagccaga ggtaaagatg atataagccg tgtggtgcgg ttgtgaaagt tgcagcggcg
    20701 cactgccctg gggggtaagc ggggcgttat agcaaaggct tgttaaattg ggaacatcgc
    20761 taaagcgcgg cagttgatcg tcggtggtaa ttaacagcga cggacgcgca tcttccagca
    20821 tcattttcag gcgatcgtcc ggatagccgg tatccagcgg tagccaggcc gcgccagctt
    20881 caactattgc atggagtgcc agggtcaaaa agaccgagcg cggtagtgcc accgccacgc
    20941 tgtcccctgg tttaacgccg cgctcacgca gcagattcgc cagcgccacc acctgctcgc
    21001 gcatttcccg atagctgaac aggtaacgcg catctgccag cgccggagca tccggtgttt
    21061 ttgccgcttg ttctgccacc agcgcgctca gcgtggtttc tggaatctca acctgagtgg
    21121 cgttgatctg cgccagctgc gcatactcac ctggcagcat aatatcgaca tcgccgcaca
    21181 acagcgccgg atccgcggcg aactgggcaa tcagcatttt caggcgttca gcatgctgga
    21241 ttaacgttgg ctcatcgtaa cgctgtttat tggcgaggat ctcaatactc aaatcaccgt
    21301 gttcatccgg gaacagggcc agttcaaggt cattaaccgg accggttgcc agggtatggg
    21361 tttgcgtctg aacaccagga atatccagtt ggtaatcaaa taccttgata ttgagtaccg
    21421 gaccgaacag cggttcctct cctgccgctc gcccgctgtc acggacaatt tgttcggcat
    21481 cgtaacgttg atgacgacgc atttttttca gttgtgctgc cagtcgggtt gccagttccg
    21541 gcagcgtttc ttgcgccgca atgtgaatac ccaacggtaa aacgttgagc acgggtccgc
    21601 tagccgtcag cgccgccgag cccagtcgac gcataaagat aaatccggcg gcatagtcca
    21661 tgcgattgca caatcgcccc agccacaagg ctgccagcgc aagggctaaa tcggtacgct
    21721 gcacacctga aagttgcgta gccagctggc ggaattcccc gtcggtaaat tccagtttca
    21781 ggcgcagaat atctgccgaa gcgctgcgcc ccgctaaagg tgccggagaa agtgacgcgg
    21841 gcggcggcag ttgacgacgc tgttctgccc agaatgccgc atcacgctgc caggcttcgc
    21901 tttcgcggta ttgctggtac tcttccacta catcagcgaa aggcgtaaac ggcgaagcag
    21961 gcgttggttc gccacgcagc catgtgcagt aaatattggc gatctggcga gtaatggccg
    22021 ggaaactgaa gccatcgacc agcaaatggt gataacgctg ataccagtac cagcggttat
    22081 ccgcaacatg tatcagctga tgaaagacca gtggtttacc gctatcgacg cgcaaatctt
    22141 gttgcaaatc cgccagcatt aatgcctgcg cagtaccgtg cggatcaata ttggttcgca
    22201 ggtcgataat ttctggcagt tcgaatatca gcgcatcatc gacccactgc cagacttcgc
    22261 cgttatcttc cgtaaaacgc atccgcaacg tatctgcttg cgctagtccg acaaccaccg
    22321 cgcgggccag taatggcgca tcaacatctc cagttaactc aacgtaatgc gccacgctcc
    22381 aggcggaggg taaatccgac agtttttctg ccatccagat gccgggctgt gcggcgacca
    22441 aaggtaaatg ctggctcatt gtgcctcctg caactgggtg aaattggccg gtgtcagagt
    22501 acgccagtgg acttccagcc actgctggca ggacgcctgt gactgcggct gacacacaat
    22561 gtcccagcct gccggtaagg cgcattgttg cggccacaga ctgaattgcc cctgcgcatt
    22621 gcgcaaaatg taaaacgctc cctgtggatc atcgaaggga ttactgaatg ccatattcaa
    22681 ctcctgtcat ggaaaagtgg ttgccagagg tcgattagcc cctgcatcaa gccaccgcgc
    22741 caacaaagcg catcatgtcc gccgtcaacc tgacgccaga aaatggattc ttttatgggg
    22801 tgtaattgcg catacagcgc ctgattggcc cgcataatca tcggctcgcg aatacccgct
    22861 tccagcacaa tgcgcagacc ttcggcgcta acttcaccag ctttaagctt ttcaagtaac
    22921 acgccctctt gctgcccgcc ccgatgcggc caccagtacg atcctgactg gcttaataca
    22981 cagccaaagc gttcaggcca gtgcagtccg gcatacagcg cggacagccc accaaaactc
    23041 tgcccggcga ccacggtgcg atcggcgcga tcgctaaaag gggcaatagc tttcaccagg
    23101 ggtaataact cttgctgtac tgccagccag aaatccgcat tacacggcag ttcgtgggcg
    23161 cggtgcgtgg tatcgatagc gtcgatcaac acatacacgg cgggaggaag ttgctgacga
    23221 tgggtcagcg aagtcagcac aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg
    23281 agcaaaactg ccagcggacg ttcttctgct gttgcatcgc cggtggtaaa aatccataca
    23341 cgacgtgaat ttttcaaccg ttcacttttc cagataattt ctttggctgc tatttctggc
    23401 gattgcggac aatcccatcc cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca
    23461 gcgtgccctc gcccgccttt ccagctttgt ggattcagcg gatcggctat tgcctggggt
    23521 aatagttttc gccagccttc acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg
    23581 tcatcgcgtt cggtgggaat aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc
    23641 gtccactgcc agacatcagt acctgcaatt cgctgcatcg actggggctg gctgttctga
    23701 tgatgatcgg tcacaccagt gatgtagatc catacgcgct ttatcgtcga gtattcttca
    23761 gaaccttggg gatcacgcca ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc
    23821 cattccgggc catgtttcga ctgccaccag ctctcacttc ctacttttaa cgccgtcaca
    23881 ccataacccc atgtttactg tgcaattttt cattgattgc agaaatatat tgataatatt
    23941 attgataact atttgcattt gcaatagcgt aatggcgcgc cgtgggaagc gcggacattt
    24001 attaaccaac tgcactgcgt gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt
    24061 cgtgttgagg acgaccatgc ccgacagttg caattcgtgg caaaaatgca ggaataaaac
    24121 aatgaacaag aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc
    24181 gcaggcacaa gagccgaccg atactcctgt ttcacatgac gataccattg tcgttaccgc
    24241 cgccgagcag aacttacagg cgcctggcgt ttcgactatc accgcagatg aaatccgcaa
    24301 aaaccccgtt gcccgcgatg tgtcggagat catccgtacc atgccaggcg ttaacctgac
    24361 cggtaactcc accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg
    24421 tccggaaaac acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg
    24481 tcagggctgg cgtggcgagc gcgatacccg tggtgatacc tcctgggtgc cacctgaaat
    24541 gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc
    24601 gggcggcgtg gttaacatca ttaccaaaaa aggcagcggt gagtggcacg gctcctggga
    24661 cgcttatttc aatgcaccag aacataaaga ggaaggtgcc actaaacgca ccaacttcag
    24721 cctgaccggt ccgctgggcg acgaattcag cttccgcttg tatggcaacc tcgacaaaac
    24781 ccaggctgac gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc
    24841 cacgacgtta ccagccgggc gcgaaggggt gatcaataaa gatattaacg gcgtggtgcg
    24901 ctgggacttc gcaccattgc aatcgctgga actggaagcg ggttacagcc gccagggtaa
    24961 cctgtatgcg ggcgataccc agaacaccaa ctctgacgct tacactcgct cgaaatatgg
    25021 cgatgaaacc aaccgcctgt atcgccagaa ctacgcgctg acctgaaacg gtggctggga
    25081 taacggcgtg accaccagca actgggtgca gtatgaacac acccgtaact cgcgtattcc
    25141 ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt
    25201 cgatatcgat ctcgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct
    25261 cgttaaccag acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttgag
    25321 ttccaatacc caggcgctga ccggggcgaa caccggcggc gctattgatg gtgtgagtgc
    25381 caccgaccgt agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaacat
    25441 ggagctgact gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt
    25501 cggcaataac tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct
    25561 caaaatgggc attgcccgtg cttataaagc accgagcctg taccagacta acccgaacta
    25621 cattctctac agtaaaggtc agggctgcta tgccagcgcg ggcggctgct atctgcaagg
    25681 taacgatgac ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg
    25741 cgacggctgg ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc
    25801 gggctatgtg gctgtagggc aaaacgcagt cggcaccgat ctctatcagt gggataacgt
    25861 gccgaaagca gtggttgaag gtctggaagg atcgttaaac gtaccagtta gcgaaacggt
    25921 gatgtggacc aataacatca cttatatgct gaagagtgaa aacaaaacca cgggcgaccg
    25981 tttgtcgatc atcccggagt acacgttgaa ctcaacgctg agctggcagg cgcgggaaga
    26041 tttgtcgatg caaacgacct tcacctggta cggtaagcag cagccgaaaa aatacaacta
    26101 caaaggtcag ccagcggttg gcccggaaac caaagaaatc agtccttaca gcattgtggg
    26161 tctgagcgcg acctgggatg tgacgaagaa tgtcagtctg accggcggcg tggacaacct
    26221 gttcgacaaa cgtttgtggc gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc
    26281 caactatatc gcgggtgccg gggcgtatac ctataacgag ccgggacgta cgtggtacat
    26341 gagcgtaaac actcacttct gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca
    26401 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaaa tcttgccaat tcaatatatt
    26461 gcaggaccat gtaggcctga taagcgtagc gcatcaggca gtttagcgtt tgtcatcagt
    26521 ctctaatatg gtcgacatga aaactacgca tacctccctc ccctttgccg gacatacgct
    26581 gcattttgtt gagttcgatc cggcgaattt ttgtgagcag gatttactct ggctgccgca
    26641 ttacgcgcaa ctgcaacacg ctggacggaa gcgcaaagcc gaacatttag ccgggcggat
    26701 cgctgcaatt tatgcgctgc gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct
    26761 acgccaaccg gtgtggcctg cggaggtata cggcagtatt agccactgcg gggctacggc
    26821 attggccgtg gtttcccgtc aaccgattgg cgttgatatc gaagaaattt tttctgcgca
    26881 aacagcgacc gaattgacag acaacattat tacaccagca gaacacgagc gactcgcaga
    26941 ctgcggttta gccttttctc tggcgctgac actggcattt tccgccaaag agagcgcatt
    27001 taaggcaagt gagatccaaa ctgatgcagg ttttctggac tatcagataa ttagctggaa
    27061 taaacagcag gtcatcattc atcgtgagaa tgagatgttt gctgtgcact ggcagataaa
    27121 agaaaaaata gtcataactc tgtgccaaca cgattaattg acaacatctg gtacgattcg
    27181 cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag gtgccttacc actctgacct
    27241 gacaaccgga tatgcgggga ttgctccccg cacaacggct acttcttcgg ttcgtaagcg
    27301 agaacagcct taaactcaat attacgttcc tttacggtga actcacacag cgagtctccg
    27361 accagaagcg taaatcccag taccgtcaaa cacagcacta tgactgccac aagggcatat
    27421 ttcgtcagca tgtttatatt gcctccatgc gaagaaggga ctaccatccg agttgttgag
    27481 gctcggagtg gcagccccag gattgataga aatatctcct ggggcttcat ctttccagac
    27541 ctcagagtta acctgaaacc ggaaagcttc aggcacccgc cgttatctta cccttcctca
    27601 acaaaatagc tgtttattta tacagtgaat cctgtttcgg acttctgtcg gcaaaactca
    27661 tcattgctct taaaacgaaa tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag
    27721 caggtgcctt accatcctga cctgacaacc ggatatgcgg ggattgctcc ccgcacaacg
    27781 gctacttctt cggttcgtaa gcgagaacaa ccttaaactc aatattacgt tcctttacgg
    27841 tgaactcaca cagcgaatcc ccgaccagaa gcgtaaatcc cagcaccgtt aaacacagca
    27901 ctatgactgc cgcaagggca tatttcgtca gcatgtttat attgcctcca tgcgaagaag
    27961 ggactaccat ccaaattgtt caggtttggg taggtagccc cagggttgat agaaatatcg
    28021 tcctggggct tcatctttcc agacctcaga gtaaacctga aaccggaaag cttcaggcac
    28081 ccgcagttat cttacccttg cttagcaaaa tagctgttta tttatacagt aagtcctgtt
    28141 tcggacttct cactccccat ttgatcaact gaagctgaac ccgttttttt ctgcatgcct
    28201 caatcgtcaa aattcttcgc caccagccag ccgtgccata acttgtcaca ttgcaaaagt
    28261 agccaaattg cttcagtcac catcaccaaa aaaagaaaca ttccagaact tccgcatctc
    28321 gcccagccgc tccgacttct atactgaata gaaaacgcca acttaagaga aacctatgcc
    28381 attacccgat tttcatgttt ctgaaccttt taccctcggt attgaactgg aaatgcaggt
    28441 ggttaatccg ccgggctatg acttaagcca ggactcttca atgctgattg acgcggttaa
    28501 aaatgagatc acggccggag aggtaaagca cgatatcacc gaaagtatgc tggagctggc
    28561 gacggatgtt tgccgtgata tcaaccaggc tgccgggcag ttttcagcga tgcagaaagt
    28621 cgtattgcag gcagccgcag accatcatct ggaaatttgc ggcggtggca cgcacccgtt
    28681 tcagaaatgg cagcgtcagg aggtatgcga aaacgaacgc tatcaacgaa cgctggaaaa
    28741 ctttggctat ctcatccagc aggcgaccgt ttttggtcag catgtccatg ttggctgcgc
    28801 cagtggcgat gacgccattt atttgctgca cggcctgtca cggtttgtgc cgcacttcat
    28861 cgccctttcc gccgcgtcgc cgtatatgca gggaacggat acgcgttttg cctcctcacg
    28921 accgaatatt ttttccgcct ttcccgataa tggcccgatg ccgtgggtca gtaactggca
    28981 acaatttgaa gccctgtttc gctgcctgag ttacaccacg atgatcgaca gcattaaaga
    29041 tctgcactgg gatattcgcc ccagtcctca ttttggcacg gtggaggttc gggtgatgga
    29101 taccccgtta acccttagcc acgcggtaaa tatggcggga ttaattcagg ccaccgccca
    29161 ctggttactg acggaacgcc cgtttaaaca tcaggagaaa gattacctgc tgtataaatt
    29221 caaccgtttc caggcctgcc gctatgggct ggaaggcgtc attaccgatc cgcacactgg
    29281 cgatcgtcga ccgctaacgg aagatacctt gcgattgctg gaaaaaatcg ccccttccgc
    29341 acataaaatg ggtgcatcga gcgcgattga ggccctgcat cgccaggtcg tcagcggtct
    29401 gaatgaagcg cagctgatgc gcgatttcgt cgccgatggt ggctcgctga ttgggctggt
    29461 gaaaaagcat tgtgagatct gggccggtga ctaagtaggg aattgcctta cagaagaata
    29521 tccctgacaa tggtgtttta acttacattt aacaatgtta tgaaacaccc tttagaaacc
    29581 ttgactaccg cagcaggcat tttgctgatg gctttcctct cttgcctgct gctgcccgcc
    29641 cccgcactgg ggctgacgct ggcacaaaaa ctggtgacca tgttccatct gatggatctt
    29701 agccagcttt acactttatt gttttgtctg tggtttttag tgctgggcgc tattgagtat
    29761 tttgttctgc gctttatctg gcgacgctgg ttctcgctgg cggattaaac gtcgattatc
    29821 gccgtacttc gcggcatact ttgcttattc tccttcgcct aaaggaacgt ttatggataa
    29881 gcaatcactg cacgaaacgg cgaaacgcct ggcccttgag ttaccctttg tcgagctttg
    29941 ctggcctttt ggcccggagt tcgatgtttt taaaattggc ggcaaaattt ttatgctgtc
    30001 gtcggagctg cgcggcgtcc cctttatcaa tctgaagtcc gatccacaaa aatccctgtt
    30061 aaatcagcag atatacccga gcattaagcc agggtatcac atgaataaaa agcactggat
    30121 ttcggtgtat cccggcgagg aaatctccga agcgttactt cgcgatctga tcaacgattc
    30181 gtggaatctg gtggttgatg gtctggctaa acgcgatcaa aaaagagtgc gtccaggcta
    30241 aagcggaaat ctatagcgca tttttctcgc ttaccatttc tcgttgaacc ttgtaatctg
    30301 ctggcacgca aaattacttt cacatggagt ctttatggat atcatttctg tcgccttaaa
    30361 gcgtcattcc actaaggcat ttgatgccag taaaaaactt accccggaac aggccgagca
    30421 gatcaaaact ctcctgcaat acagcccatc cagcaccaac tcccagccgt ggcattttat
    30481 tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa tccgctgccg gtaattatgt
    30541 gttcaacgaa cgtaaaatac ttgatgcctc gcacgtcgtg gtgttctgtg caaaaaccgc
    30601 gatggacgat gcctggctga agctggttgt tgaccaggaa gatgctgatg gccgctttgc
    30661 cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag ttcttcgctg atatgcaccg
    30721 taaagatctg catgatgatg cagagtggat ggcaaaacag gtttatctca acgtcggtaa
    30781 cttcctgctc ggcgtggcgg cgctgggtct ggacgcggta cccatcgaag gttttgacgc
    30841 cgccatcctc gatgcagaat ttggtctgaa agagaaaggc tacaccagtc tggtggttgt
    30901 tccggtaggt catcacagcg ttgaagattt taacgctacg ctgccgaaat ctcgtctgcc
    30961 gcaaaacata accttaaccg aagtgtaatt ctctcttgcc gggcatctgc ccggctattt
    31021 cccctcagat tctcctgatt tgcataaccc tgtttcagcc gtcatcatag gctgctgttg
    31081 tataaaggag acgttatgca ggatttaata tcccaggttg aagatttagc gggtattgag
    31141 atcgatcaca ccacctcgat ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg
    31201 gtgcatatta ttttgcattg ggtggtactg cggaccttcg aaaaacgtgc catcgccagt
    31261 tcacggcttt ggttgcaaat cattacccag aataaactct tccaccgttt agcttttacc
    31321 ctgcagggga ttatcgtcaa tattcaggcg gtattctggc tgcaaaaagg caccgaagcg
    31381 gcagatattc tgactacctg cgcgcagttg tggatcatga tgtatgcgct gctttcagtc
    31441 ttctcgttgc tggatgtgat tttgaatctg gcgcagaaat tcccggcggc atctcagtta
    31501 ccgctgaaag ggatatttca ggggattaaa ctgatcggcg cgattctggt cggcattttg
    31561 atgatctcgc tgctgattgg tcagtcgcca gcgattctga tcagcggtct tggtgcaatg
    31621 gctgccgtgc tgatgttggt ttttaaagat ccgattcttg gtctggtggc aggtattcag
    31681 ctttccgcga acgatatgct gaaactgggc gactggctgg agatgccgaa atacggcgcg
    31741 gatggcgcgg tgatcgatat tgggttaacc accgtcaaag tgcgtaactg ggacaatacc
    31801 attaccacta ttcccacctg gtctctggtt tctgactcct ttaaaaactg gagcgggatg
    31861 tcagcatctg gcgggcgacg tattaagcgc agtatcagta ttgatgtcac cagtattcgt
    31921 tttcttgatg aagacgaaat gcaacgtctg aataaagcgc atttgttaaa gccttattta
    31981 accagccgcc atcaggaaat taatgagtgg aatcgccagc aaggttctac ggagtcgata
    32041 ttaaacctgc gccgaatgac caatattgga acctttcgtg cctatctgaa cgaatatctg
    32101 cgtaaccatc cgcggattcg taaagatatg actttaatgg tacgccaact ggctccaggt
    32161 gataacggtt taccgctcga gatctatgcg tttaccaaca ccgtggtgtg gctggaatat
    32221 gaaagcattc aggctgatat attcgatcac atatttgcga ttgtcgaaga gtttggtctg
    32281 cgacttcatc agtcgccaac cggcaatgat attcgctctc tggcgggtgc atttaagcag
    32341 taattaaaaa aaccgctctc atcgaatgga tgagagcgga catcgttttg tcggatgtag
    32401 cgtgaatgcc ttatttccga cgcagcgttt taaatgccac aaacaggaat acaatccaca
    32461 ccggcagcag gatcgctgac aagcgcatat catccatcgt gcacatcagc agcaaaatca
    32521 tgccgaggaa ggcaatgcaa agatagttgc cgaacggata gagcagcgcc ttaaactgtg
    32581 tttcacgccc ctgacgtcgc atcgctgcac gaaaacgcag atgcgccaga cagatcataa
    32641 tccagttcaa cagcagcgtt gctaccacca gcgccatcag cagaccaaac gctttttgcg
    32701 gcagcagata gttgattaac accaccagcg aagtgatcgc tccggaaagc atcagcgagt
    32761 taatcggcac accgcgacgg ctgacgcgag tcaaaaactt cggcgcatta ccctgcacag
    32821 aaaggccaaa cagcatgcgg ctgttagagt aaaccccgct gttatacact gacagcgatg
    32881 ctaccagaat gacgaagttc agcgcagaag ctaccacgtt gctgtcgaga ttatggaaaa
    32941 tcatcacaaa cgggctactg ttggatttca cttccaccca cggatagagc gccagtaaaa
    33001 ccaccagtga accgatgtaa aacagcagga tgcgatacac cacctgattt accgcttttg
    33061 gaatgctttt ttccggatcg cgcgcttcag cggcagtaat cccaatcagc tccagaccgc
    33121 cgaaggagaa cataattacc gccagcgaca aaatcagccc attccagccg gtggcgaaga
    33181 aaccaccgta gcgccagagg ttgtcgatac tggctttctc gccgccgtga ccagaaaaca
    33241 gcagccacag gccaaagccg atcataccga tgatcgccag cactttaatc aacgcaaacc
    33301 agaactcggt ttcgccatat aagcgcacgt tcaccaggtt aacggcgttg atgataataa
    33361 agaaggcggc agcccaaatc cacgttggaa catccgggaa ccagtactgc atatagatgc
    33421 ccgcagcggt cagctctgcc atccccacca gcacgaacat cacccagtag ttccagccag
    33481 agaggaagcc cgcgaacggt ccccagtatt tataggcaaa gtgggcaaac gaaccggata
    33541 ccggctcttc gaccaccatc tcgccgagct ggcgcataat caggaaagcg atgatcccgg
    33601 cgacgccgta gcccagcaat acagccggac ccgccatctg aatcgccggg ccaatgccaa
    33661 gaaacagacc agtaccaatt gcgccaccca gcgcaatcag ttgaatatga cggttatgta
    33721 atccgcgatg aagcgtcggc tcttgattcg acgcagtatc ttccgatacg gttgacgcgt
    33781 ttttcacgcc tttcccctgt gtgtcttttt tgttgagggg caccttttaa catttagtgc
    33841 ccatcgtcgc aagacacaat ccacacggtt aaacggggta tcctgctttt atttccgtac
    33901 ccgatgtcgg tgcagccaca tcagcttata cgccgccggg ataataaaca gcgacagcaa
    33961 aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc cggctcatca cctctgaacc
    34021 agcccccgtt ccccacagaa tcggcagcag accggcgata atcaccgcca ccgtcatcgc
    34081 tttcgggcgc acgcgcagta ccgcgccgtg atacaacgcc tcatccagct tctgctcact
    34141 gaatgtttgc ggattattca acgacggctc ggcctctatg gcgtgacgta aatacatcag
    34201 catcaccacg ccaaattcgg cggcgacccc ggcaagggca ataaagccag tacccgtcgc
    34261 cacagaaaga tgaaagccca tccaccacag caaccagatg ccgcccacca gcgcaaacgg
    34321 tacgctgctg ataatcagca acgcttcgcc cacccgacgg aatgccagat acaacagcac
    34381 gaagatgatc atcagtgtca tcggcaccat gagtttcagc ttatggttgg cgcgctccag
    34441 tagctcgaac tgcccggaga atgccacgct ggtgcccggt tttaactgca ctttttcagc
    34501 tatcgctttt tgcaaatcgt gaaccaccga caccatgtca cgatcgcggg catcgatata
    34561 aatccagctc gtcgggcgcg cattctcggt tttcagcatc gacggtccgg tagagacttt
    34621 aacgtcggcc acgtctgcca gggtgatttg ctgcttcatc ggcgtcagga tcggcaactg
    34681 gcgcagtgcc tgcggactat cgcgccagct ttgcggataa cgcagattaa tgggataacg
    34741 ggcaatccct tccaccgttt cgccaaccat cgccccgccc accgcagaag tcacaaacaa
    34801 ctgcacatcc gccaccgtca taccgtaacg cgcggctttt tcacggttaa tctcaacgtt
    34861 gatatagcgc ccaccttcca gacgctcggc aagcgcagaa gccacgcctg gtaccgttcg
    34921 tgccacctct tcaatttgct ccgccatcgc gtcgatatcc gccagcacag tgccggaaac
    34981 tttaatgccg atggggcttt taatgccagt cgagagcata tcgatacggt tacgaattgg
    35041 cggcacccac agattcgcca gccccggcag ccgcacggtg ttatccagtt cctcaatgat
    35101 tttgtccatc gtcatgccag gccgccactg atcctgcggc ttaagctgga tggtcgtttc
    35161 taccatctcc agcggagcag aatcggtggc ggtttccgct ttcccggttt tgccaaatac
    35221 ccgcgccact tcaggtacgc tcataattag cttgtcggtt ttttgcagca tactcgccgc
    35281 ctctgctgcg gaaatccccg gcagcgtcga tggcatatac aacaagtcgc cttcattgat
    35341 ctgcggtaaa aattccccgc caactttatt gagcggccag agaaccgtca gcaccgaaag
    35401 cgctgccacc agcagcgtgg ttttcggcca gtgcagtact ttcagcaaca gcggatgata
    35461 aacacgaatc aaaaagcgat tgagcgggtt actgctttcc ggcggaattt tgccacggat
    35521 ccagtacccc atcaggatag ggatcactac gatcgccagc agcgccgcgc ccgccatcgc
    35581 ataggtttta gtgaacgcca acgggccaaa cagacgccct tcctgccctt ccagggtgaa
    35641 gatcgggata aacgacaacg tgataatcag cagactgata aatagcgccg gacccacttc
    35701 aacagatgca tcggtgatca cctgccagcg cgttttatta tccagcgtgg cgtcaggatg
    35761 ctggtgctgc cactcttcca gccgtttatg tgcattttcg atcatgacga tagcggcatc
    35821 gaccatcgcc ccgacggcaa tcgcaatgcc gcccaacgac ataatattgg cattcagtcc
    35881 ctggaagtgc atgacaataa aagcaataca caaccccagc ggcaacgaaa taatcgccac
    35941 cagcgccgag cgcacatgcc agagaaacag cgcacagact accgccacca caataaactc
    36001 ttccagcaac ttgccgctga ggttgtcgat ggcgcggtca atgagctggc tgcgatcgta
    36061 tgttgtaact atctccacgc cttccggcag actacttttc agcgtttcca gtttgtcctt
    36121 cacggcggcg atcacttctc gggcgttttt gccggatcgc aggatcacca ccccgcccgc
    36181 cacttcgcct tcgccgttta gttcggcaat gccccggcgc atctccgggc caatctggac
    36241 cttcgcaaca tcgcgcagat aaacgggcac gccattttca ctggctttta aaacgatgtg
    36301 attaaagtcg tcgagcgttt gcagatagcc gctggcgcgc accatatatt ccgcttccgc
    36361 cagttcgatc gacgaaccgc ccgcttcctg gtttgaagca tccagcgcgc tttttacttc
    36421 ggcgagactg atgccatact gcgccaggcg ctggggatcg ataaccacct gatactcttt
    36481 caccacaccg cccaccgacg ccacttccgc aacgtcaggg atggttttca gctcatattt
    36541 gagaaaccag tcctgcaatg agcgtaaatc ggccagatcg tgcttaccgc tgcgatccac
    36601 cagtgcatat tcatagatcc agccaacacc cgtggcatct ggtcccagct cggcgctgac
    36661 tcccgcaggc agcttaccct gtacctggtt gaggtactcc agcacccgcg aacgcgccca
    36721 gtacggatcg gtgccatctt cgaaaatgac atacacataa gagtcgccaa actgtgagaa
    36781 accgcgcaca gtcttcgcgc caggcaccga caacatggtg gtggttagcg gataagtcac
    36841 ctgattttca acgatttgtg gtgcctgacc gggatagctg gttttaataa taacctgcac
    36901 atcggagaga tccggcagcg catccactgg cgtattaatg atggtccagg tgccccagat
    36961 gctcagaaac aacgcgccca tcagcaccaa aaaacggttc gccaccgagc gacgaataat
    37021 ccattcaatc attggttatt ccctcaatgc gcatgggtag cactttcaga gcgcatccgc
    37081 tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga acaggccgct ggaaaccacc
    37141 ttttcacctt ccgccagacc agagcgtaat gcggtgacgc cttgcgatgc ctggaaaaca
    37201 gcaacgcgtt tcggtacaaa gcgcccgtcg gcatcaacgg taatcacccg ctgttcgctg
    37261 ccggtatcaa tcagcgcttg tgacggaatg agcagcatcg gttcgctggc ggtgttgagt
    37321 tgcaaccagg cgttcatgcc cggttttagc gcctcgtcgg cgttgtcgac ttccagacgc
    37381 agctgcagcg tgcgggtcgc ggcatccacg ccaggtagca gcgtccattt gcggatggtg
    37441 agtgttttat ccggtcgcgc cggaacggtg agggtaaact gcgaggcatc tttcaccagc
    37501 caggcgatgg actccgggat cgcagcagtg acccacaccg ggtccatacc ctgaattttc
    37561 gcgaccacgt tatctttggc gatattcatt cccgcgcgca gatcaaacgc ggtgatcacg
    37621 ccatcaatgg gcgctttgag cgtaaagcga gtctggattt tttgcgtggc gatcaggcgg
    37681 cgaatatccg cctccggcat tcccgccagt cgcagccgct caagaatgcc ttcagtctgg
    37741 gtcgccgtac cgccggtttc gcgcagcagt aaatactcag tctgcgcttc cacccagtca
    37801 ggaatggtca ggtcgagaag cggtgtgccc ttttgtactt tatcgcccac ggtaagcgga
    37861 tacaccttgt cgataaaccc ggcagcgcga gcctgcacaa tggcatactg atactcgttg
    37921 taactgacat tcgccgggaa actctgggca aaagtcagcg gtccgcgcgt gacggtagcc
    37981 gttttcaccc ccagattctg cgtctgagtc gggtcaatgc gcacaccaga cgcagaactc
    38041 tcttcatcgg catatttcgg caccagatcc atatccataa acggcgattt acctggttta
    38101 tcgaaccgcg tattgggata catcgggtcg taccagaata agattttacg ttctgcggtc
    38161 gacgtttttt ctgcaggcgg ttccgccttt gcaacccagg taaaacctgc cgcagaaata
    38221 ataccgcccg cgatcatgct gccgataata agcgcgattt ttttcatctc attaaacctg
    38281 ggttactggc tgactttaat atcctgtaat aaagaaaggt tgccctgctg gacaaaatta
    38341 aacgccactt tgtcgccagt tttaatttca ctcattttcg tctgcggagt gatggtaaag
    38401 cgcatggtca tctccggcca gttcacggca gcaatcggat cgtgatggat ggtgattttt
    38461 ttgctttcca gatcaatacc ctttaccacg ccagtggcgc taataacctg cggttgtact
    38521 tcgctcatgg tttcatgatg atgttcgtta gcctgggcat taaagccaat aacggtaaac
    38581 agactgaaca ttgcgacttg cagtgctttt ttcatttatt ctctcctgga gttaaaagtt
    38641 attgctgcca accgccacca agtgcggtat ataaagaaat ttcgttaacc tgacgggcat
    38701 aattcaggtc aagtaaagtt tgtcgggttg caaataacga acgttcggca tccagcactt
    38761 ccagataact tactgcgccg tgctgatata atgcccgcgc ccgttgcaaa gtaatttgca
    38821 gcgatgccag ataacgctgc tgggcgctaa tttgatcgtt caggctttga cgtaatgcca
    38881 gcgcatctgc cacttcttta aaggcgttct ggattttctg ttcataattc actaccgact
    38941 gctgctggcg aatttcggcg atatccagat tggcctggtt gcgtccggca ttaaaaatgg
    39001 ggatctcaat tttaggaata aaattccaca tcccgctgct ggcgttaaat aatgacgaca
    39061 gatcgctgct ggcggttgat attccgctgg tcaggcttat agatgggaaa aatgccgcgc
    39121 gtgcggcccc aatattggca ttagccgcca ttaacgcgtg ttcagcttcc ataatatccg
    39181 ggcgctgcaa taagatttgc gacgacaagc ccgccggtaa tttaacgctt tgcaggctgt
    39241 cgctgtttac tgtctgcgct tgcggcagct tgccgtagct tcccagcaac agttgcaatg
    39301 cattattcgc ctgcgccagt tccccctgac gtttagcgat gtcgctgcgg gtactttcta
    39361 tcaccccgcg agcctgttcc agcgccagaa cattactgct acctgtcaac agttgttttt
    39421 cgacaaacgc atatgactgc tgataattac gcagcgtttc ttcagctatt tgcaattgcg
    39481 catacgccag ttgctgattg aaatagcttt gcgcgacatt agaaaccagc agaatatgca
    39541 ccgcgcgctg agcttcctca gtggctaaat aattttggcg ctcggcttcg ctcatgttct
    39601 ttaagcgacc gaaaaaatcg aggtcaaagc tggcgttaag gccggtcgaa aactcccgtg
    39661 tcgtggcggt attcccttta agattaccgc tccagctgcc gctgccctcg ccattgagct
    39721 gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg tgcctcctgc actttcagcg
    39781 tcgccatgcg caaatcccgg ttattcacca gcgcctcgct aatcagcgtc ttcacctgat
    39841 tatcaacaaa aaaggtgcgc cagcccgcgt tctgatagtt atctgctgcg ttaacgaggc
    39901 cgttctggct gagtgagaac tgctgcggta cgggcattgc cgggcgctga taatccggtg
    39961 ccagtgaaca accggttagc gcaagggcca cacaaaatgg cagaagttta caaggagaca
    40021 taggctcata atttctgatg attttatgcc gccaacttta ctcgccaggc tctgattttc
    40081 cggtgacagg aaaatgacaa aattgtcatt ttgccaataa gcgattgcca tctgatcccg
    40141 ctactctaga attgcccggg caacatgcgg aggaaatatg aaactgttga ttgtcgaaga
    40201 tgaaaagaaa accggagaat acttgaccaa agggttaacc gaagccggtt ttgtggtcga
    40261 tttggccgac aacgggctga atggctacca tctggcgatg accggtgatt atgatctgat
    40321 aatcctcgat attatgctgc cggacgtgaa cggctgggat atcgtgcgca tgttacgctc
    40381 cgccaataaa gggatgccga ttctgttgct taccgcgctt ggcaccattg aacatcgcgt
    40441 caaggggctg gagttggggg cagatgacta cctgatgaaa ccattcgctt ttgctgaact
    40501 gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg gtaattatcg aaagtcagtt
    40561 tcaggttgcc gacctgatgg tcgatctcgt cagccgcaaa gtcacccgca gcggcacgcg
    40621 catcactttg accagtaaag agtttactct gctggagttt tttctccgcc atcagggcga
    40681 agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac atgaatttcg acagcgacac
    40741 taacgctatt gatgtagcgg tgaagcggct gcgcggcaaa atcgacaacg actttgagcc
    40801 aaagctaatt cagaccgtgc gcggcgtggg ttacatgctt gaggtgccgg atggtcagta
    40861 agccatttca gcgcccgttt tcgctggcaa cccgcctgac cttttttatc agcctggcca
    40921 ccatcgcagc gtttttcgcc tttgcatgga tcatgatcca ctcagtaaaa gtgcattttg
    40981 ccgagcagga tattaatgat ttaaaagaga ttagcgccac gcttgaacgg gtacttaatc
    41041 accctgacga aacgcaagcc cgacgcttaa tgacgctgga agatatcgtc agtggttatt
    41101 ccaacgtgtt gatttccctg gcagatagcc acggcaaaac ggtgtatcac tcccccggtg
    41161 cgcctgatat ccgcgagttt gcacgtgatg ccatacccga taaagacgcg cggggtggcg
    41221 aggtgtatct cctttccggc ccgacgatta tgatgccagg ccacggtcac gggcatatgg
    41281 aacacagcaa ctggcggatg attaacttgt cggttggccc gttggtggac ggcaaaccga
    41341 tttatacgct ctacatcgcg ctttcgatcg attttcatct tcattacata aatgatttga
    41401 tgaataaact tattatgacc gcatcggtaa tcagcatcct gatcgtcttt atcgtactgt
    41461 tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag ccgtcaaatc cagaatatca
    41521 cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt gcccattgag ctggaacagc
    41581 tggtactgtc gttcaaccat atgatcgagc gtattgagga tgtctttacc cgccagtcca
    41641 atttctcagc ggatatcgct cacgaaattc gcacgccgat taccaatctc atcacgcaaa
    41701 cggaaatcgc cctcagccag tctcgcagcc agaaggagct ggaagatgtg ctctactcta
    41761 atctcgaaga gctgacgcga atggcgaaaa tggtcagcga tatgctgttt ctcactcagg
    41821 ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa cctggcggat gaagtcggca
    41881 aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg cgtggagttg cgatttgttg
    41941 gcgacgaatg tcaggttgcg ggcgatccgc tgatgctgcg tcgggcgtta agcaacctgc
    42001 tttctaatgc cctgcgttat acgccgcccg gagaggcaat tgtagtgcgc tgccagacgg
    42061 tcgatcacca ggtgcaagtt accgtcgaaa accccggtac gcccattgcg cccgagcact
    42121 taccgcgatt gtttgaccgt ttctaccgcg ttgacccttc ccgccagcga aaaggcgaag
    42181 gtagcggcat tgggctggcg atagtgaaat cgattgttgt cgcgcataaa ggcacggttg
    42241 cagtaacgtc agatgcgcgg gggacaaggt ttgtgattat gctgccggag agagagtgat
    42301 tattaagttc aaattcaggg cgtggtg
//
LOCUS       DAZFFI010000030        41517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFFI010000030 DAZFFI010000000
VERSION     DAZFFI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.030"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            214..633
                     /gene="umuD"
                     /locus_tag="ACHJ12_001925"
     CDS             214..633
                     /gene="umuD"
                     /locus_tag="ACHJ12_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4989717.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            633..1901
                     /gene="umuC"
                     /locus_tag="ACHJ12_001926"
     CDS             633..1901
                     /gene="umuC"
                     /locus_tag="ACHJ12_001926"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO4989718.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="ACHJ12_001927"
     CDS             complement(1947..2477)
                     /gene="dsbB"
                     /locus_tag="ACHJ12_001927"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4989719.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="ACHJ12_001928"
     CDS             complement(2623..4164)
                     /gene="nhaB"
                     /locus_tag="ACHJ12_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4989720.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISSFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4386..5105
                     /gene="fadR"
                     /locus_tag="ACHJ12_001929"
     CDS             4386..5105
                     /gene="fadR"
                     /locus_tag="ACHJ12_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4989721.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5157..6689)
                     /gene="ycgB"
                     /locus_tag="ACHJ12_001930"
     CDS             complement(5157..6689)
                     /gene="ycgB"
                     /locus_tag="ACHJ12_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4989722.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7019..8317
                     /gene="dadA"
                     /locus_tag="ACHJ12_001931"
     CDS             7019..8317
                     /gene="dadA"
                     /locus_tag="ACHJ12_001931"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4989723.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8327..9397
                     /gene="dadX"
                     /locus_tag="ACHJ12_001932"
     CDS             8327..9397
                     /gene="dadX"
                     /locus_tag="ACHJ12_001932"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO4989724.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9783..11519)
                     /gene="cvrA"
                     /locus_tag="ACHJ12_001933"
     CDS             complement(9783..11519)
                     /gene="cvrA"
                     /locus_tag="ACHJ12_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4989725.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11614..12528)
                     /gene="ldcA"
                     /locus_tag="ACHJ12_001934"
     CDS             complement(11614..12528)
                     /gene="ldcA"
                     /locus_tag="ACHJ12_001934"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4989726.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12628..13239
                     /gene="emtA"
                     /locus_tag="ACHJ12_001935"
     CDS             12628..13239
                     /gene="emtA"
                     /locus_tag="ACHJ12_001935"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4989727.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13241..13983)
                     /gene="ycgR"
                     /locus_tag="ACHJ12_001936"
                     /pseudo
     CDS             complement(13241..13983)
                     /gene="ycgR"
                     /locus_tag="ACHJ12_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            14184..14438
                     /gene="ymgE"
                     /locus_tag="ACHJ12_001937"
     CDS             14184..14438
                     /gene="ymgE"
                     /locus_tag="ACHJ12_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4989728.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14616..15056
                     /gene="ycgY"
                     /locus_tag="ACHJ12_001938"
     CDS             14616..15056
                     /gene="ycgY"
                     /locus_tag="ACHJ12_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO4989729.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15135..16832)
                     /gene="treA"
                     /locus_tag="ACHJ12_001939"
     CDS             complement(15135..16832)
                     /gene="treA"
                     /locus_tag="ACHJ12_001939"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4989730.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17152..18570)
                     /gene="dhaM"
                     /locus_tag="ACHJ12_001940"
     CDS             complement(17152..18570)
                     /gene="dhaM"
                     /locus_tag="ACHJ12_001940"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO4989731.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18581..19213)
                     /gene="dhaL"
                     /locus_tag="ACHJ12_001941"
     CDS             complement(18581..19213)
                     /gene="dhaL"
                     /locus_tag="ACHJ12_001941"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO4989732.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19224..20294)
                     /gene="dhaK"
                     /locus_tag="ACHJ12_001942"
     CDS             complement(19224..20294)
                     /gene="dhaK"
                     /locus_tag="ACHJ12_001942"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO4989733.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20522..22441
                     /gene="dhaR"
                     /locus_tag="ACHJ12_001943"
     CDS             20522..22441
                     /gene="dhaR"
                     /locus_tag="ACHJ12_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO4989734.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22541..25426)
                     /gene="ycgV"
                     /locus_tag="ACHJ12_001944"
     CDS             complement(22541..25426)
                     /gene="ycgV"
                     /locus_tag="ACHJ12_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4989735.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVIGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNDGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNIGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGKSQLNMYVKTGAIHEFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNTQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(26268..27359)
                     /gene="ychF"
                     /locus_tag="ACHJ12_001945"
     CDS             complement(26268..27359)
                     /gene="ychF"
                     /locus_tag="ACHJ12_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4989736.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27476..28060)
                     /gene="pth"
                     /locus_tag="ACHJ12_001946"
     CDS             complement(27476..28060)
                     /gene="pth"
                     /locus_tag="ACHJ12_001946"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4989737.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28338..28616
                     /gene="ychH"
                     /locus_tag="ACHJ12_001947"
     CDS             28338..28616
                     /gene="ychH"
                     /locus_tag="ACHJ12_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4989738.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28671..30350)
                     /gene="dauA"
                     /locus_tag="ACHJ12_001948"
     CDS             complement(28671..30350)
                     /gene="dauA"
                     /locus_tag="ACHJ12_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4989739.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VAVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30475..31422)
                     /gene="prs"
                     /locus_tag="ACHJ12_001949"
     CDS             complement(30475..31422)
                     /gene="prs"
                     /locus_tag="ACHJ12_001949"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4989740.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31573..32424)
                     /gene="ispE"
                     /locus_tag="ACHJ12_001950"
     CDS             complement(31573..32424)
                     /gene="ispE"
                     /locus_tag="ACHJ12_001950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4989741.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32424..33047)
                     /gene="lolB"
                     /locus_tag="ACHJ12_001951"
     CDS             complement(32424..33047)
                     /gene="lolB"
                     /locus_tag="ACHJ12_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4989742.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33261..34517
                     /gene="hemA"
                     /locus_tag="ACHJ12_001952"
     CDS             33261..34517
                     /gene="hemA"
                     /locus_tag="ACHJ12_001952"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4989743.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNQAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34559..35641
                     /gene="prfA"
                     /locus_tag="ACHJ12_001953"
     CDS             34559..35641
                     /gene="prfA"
                     /locus_tag="ACHJ12_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4989744.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35641..36474
                     /gene="prmC"
                     /locus_tag="ACHJ12_001954"
     CDS             35641..36474
                     /gene="prmC"
                     /locus_tag="ACHJ12_001954"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4989745.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36471..36863
                     /gene="sirB2"
                     /locus_tag="ACHJ12_001955"
     CDS             36471..36863
                     /gene="sirB2"
                     /locus_tag="ACHJ12_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4989746.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36867..37676
                     /gene="sirB1"
                     /locus_tag="ACHJ12_001956"
     CDS             36867..37676
                     /gene="sirB1"
                     /locus_tag="ACHJ12_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4989747.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37712..38566
                     /gene="kdsA"
                     /locus_tag="ACHJ12_001957"
     CDS             37712..38566
                     /gene="kdsA"
                     /locus_tag="ACHJ12_001957"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4989748.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38715..38822)
                     /gene="ldrA"
                     /locus_tag="ACHJ12_001958"
     CDS             complement(38715..38822)
                     /gene="ldrA"
                     /locus_tag="ACHJ12_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989749.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39250..39357)
                     /locus_tag="ACHJ12_001959"
     CDS             complement(39250..39357)
                     /locus_tag="ACHJ12_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989750.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39786..39893)
                     /locus_tag="ACHJ12_001960"
     CDS             complement(39786..39893)
                     /locus_tag="ACHJ12_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4989751.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(40298..41398)
                     /gene="chaA"
                     /locus_tag="ACHJ12_001961"
     CDS             complement(40298..41398)
                     /gene="chaA"
                     /locus_tag="ACHJ12_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4989752.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT        10344 a        10355 c        10628 g        10190 t
ORIGIN      
        1 tggcgaatgc ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag
       61 cgtcgtcgcc agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctatg
      121 cagcgacaaa tattgatagc ctgaatcagt attgatctgc tggcaagaac agactactgt
      181 atataaaaac agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc
      241 cgcgaaattg tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg
      301 gcagcagatt acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc
      361 gcgacttact tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt
      421 gatttactga ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct
      481 gttgacggcg agtttacggt gaaaaaattg caactacgcc cgacggtaca gcttattccc
      541 atgaacagtg cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt
      601 gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt
      661 tttatgccag ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc
      721 tatcgaataa tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta
      781 aaatgggcga tccctggttc aaacaaaaag atctgtttcg tcgctgtggc gtggtttgct
      841 ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag
      901 aactatcgcc ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg
      961 tgcgtaattg tcgcgatctg actgattttg gcagagaaat tcgcgcaacg gtgctacaac
     1021 gtacccatct tactgttggt gtggggatcg cccagaccaa aacgttggct aagcttgcca
     1081 atcatgcggc aaaaaaatgg cagcggcaga cgggtggggt ggtggattta tcaaatctgg
     1141 aacgccagcg taaattaatg tctgctctcc ccgtggatga cgtctggggg attggacggc
     1201 ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt tctcgatttg gcggatacag
     1261 atatccggtt tatccgtaaa cattttaatg tcgtgctcga aagaacggtg cgtgaactgc
     1321 gcggcgaacc ctgtttgcaa ctggaagagt ttgcaccgac gaagcaggaa attatctgtt
     1381 cccgctcgtt tggtgaacgc atcacggatt atccatcgat gcggcaggcc atttgtagtt
     1441 acgctgcccg ggcggcggaa aaacttcgca gcgagcatca atattgtcgg tttatctcca
     1501 catttattaa gacgtcacca tttgcgctca atgaacctta ttacggcaat agcgcgtcgg
     1561 taaaactgct gacgcccact caggacagca gggatatcat taacgctgct acgcgatctc
     1621 tggatgccat ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg ctgggggatt
     1681 tcttcagtca gggagtcgcg cagctcaatt tattcgatga caacgcaccg cgccccggga
     1741 gtgagcaatt gatgacggta atggatacac tgaatgctaa agagggcaga ggaacactct
     1801 attttgccgg gcaggggatc cagcaacaat ggcagatgaa gcgtgccatg ctttcaccac
     1861 gttatacaac gcgaagttct gatttactgc gggtcaaata aatatagcgg caggaaaaaa
     1921 gcgctcccgc aggagcgctg aagggattag cgaccgaaca ggtcacgttt tttcgcttta
     1981 aacggctggg aaatcaccac cagcaccgcg acaatcaggt aagcgataaa aataccgagc
     2041 agccactgcg gcatttccag acctaaaaaa tcccactgac gctcggcaca atcgccagag
     2101 gcgacaaaca cttgcggcac ccatttatcc agcggcagcc attccgggaa acgaaccata
     2161 aaatcacagg tggcaaacgg cgaaggatag agctgaagca tggtgtgctc gtaagttaac
     2221 tgcacaccgc ggaacgcact atacaaccag ataaccatcg ctacataacg cagcggagtt
     2281 ttcggggcga tcgcgccaat cagcgcagca cccagaacgc cgaataacgc acaacgttca
     2341 taaatacaga gcacgcaagg tttcagtaac atcacatgct ggaaccacag cgccgtcagt
     2401 tccagtgcca gagcagtaaa cgccatcaac agccacgcgc cccggccttg tgaacattgg
     2461 ttcaaaaatc gcaacataat catttccctg caatatgcat agagtgcgca gtttaaacca
     2521 attcattcgc tgcgccacca ggaggcagca aaaataggaa gcaattggac aattatcggg
     2581 cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg ttgctatcca
     2641 gcccatttgc ataaaccatt cggttacagg ggcaagcgta aactcgacgc agagcaagcc
     2701 gacgagtgtc agtacgaggg tgtaaggcag ggccatccac accatgcggc cataagagag
     2761 gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg aacgcagcct gaccgttcgg
     2821 cgtagcgacg gagggcagat tggtaccggt attaatggcg accgccagca gctcgtattg
     2881 cttcaacgtg atagcgccac tttccattgc cgctttcgct tcgttgatat aaatcgtccc
     2941 cacgaagacg ttatccgaaa tggatgacag cagaccgttg aaaatataga acagcgacag
     3001 ctgagcatgt tccgatgcct gcaacacaaa ctgaataatt ggcgaaaaca gttgttggtc
     3061 gataatcacc gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg gcagagattc
     3121 ggtgaaggct ttaccgatag catgttcatc ggtgacaccg gtcaatgatg tcgccagaat
     3181 aatgactgac aaaccaatca agccaacttc cgccagatgc aacgccagcg cagtcaccag
     3241 ccagacgcca ataatcgcct ggacaatcag acggatttta tcctgacggg tacgctggtg
     3301 gcggctttga tcgtcaaact gttgcaacac ttcgcggact ttctccggca gcgtttcacc
     3361 gtagccaaac caacgcagct tctctaccag caggcaggtt aacaggccac aaatcagaac
     3421 cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc cagccgcttt
     3481 agcgatgatc aggttctgtg gttcgcccac catggtcatc acgccgccta atgcagtgcc
     3541 gacaccggca tgcatcatca ggctacgcag aaagccacgg aactgttcca gaaccacttt
     3601 gtaatgcttg tcgatatgac tatcgtcttg caggtcggta tcttcggtac gggaagaggc
     3661 tacgcggtga taaataccat aaaaaccgac agcaacgctg atcaccaccg ccacgacggt
     3721 taaggcatcg aggaacgcgg agaggaacgc tgccgccacg caaaaagaga gcgacagcag
     3781 cattttggag cgaatgctta acagcaaacg ggtaaatatg aacagcaaca gctgtttcat
     3841 aaaatagata cccgccacca taaacatcaa taacagcaag acttcaagat ttgccgccac
     3901 ctcttcacgg acgtgttccg cgctggtcat gccgatgaat accgcttcga tagccaacag
     3961 accaccgggg agcagcgggt agcatttcag ggccatcgcc agagtgaaaa taaattccgc
     4021 taccagcaac cagcccgcga cgaaagagct gatgaggaaa attaacgggt ttacgattaa
     4081 gaaaatgatg agggcgagtt tgtaccagtc gggggactgg cccaaaaaat tgcgccatag
     4141 cgcgcggccc caggagatct ccatgatggt ttcccttacc ttacaaataa tcaatgatgt
     4201 ttttatgttt aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc ttgatagtca
     4261 aggggaaaga gatgcggaaa atgaagcctt gatccctttt ttcttctttt tgtctgctat
     4321 cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc
     4381 tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa
     4441 gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag
     4501 aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg
     4561 gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacctctg
     4621 gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg
     4681 ataatttgct gtcggtgcgt accaatattt ccactatttt tattcgcacc gcgtttcgtc
     4741 agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg
     4801 atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc
     4861 cgatttacgg tctgatcctt aacgggatga aagggctgta tacgcgcatt ggtcgtcact
     4921 atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt
     4981 tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga
     5041 gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc
     5101 gataatccct tccgtttaaa gagcaaaccc ctcaaacgag gggttttttg ttgtttttac
     5161 agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc
     5221 tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcagcac ttctttgcgc
     5281 ccccgatcca gcggtgcgcg attatgtgga atgtaacgca gcgtcagcga acggtcgccg
     5341 cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac
     5401 gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga
     5461 taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt
     5521 gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt
     5581 tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac
     5641 tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg gttgatgccg
     5701 ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac
     5761 tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag
     5821 tgccagaagg tcgcccagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac
     5881 tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca
     5941 tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca
     6001 gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt
     6061 tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc
     6121 gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt
     6181 tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc
     6241 agataatcga caatcgaact ggcgtcggtc caactgcgga ataagtaatt gtttttaaag
     6301 aaagagttat gcccatagca ggcgtgtgcc atcaccagcg cctgcatggt aatggtgttc
     6361 tcttccatca ggtaagcgat acacgggtta gagttaatga cgatttcata ggccagtcct
     6421 tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc
     6481 ggatagttaa ttggcatgcc gacgctggag taggcatcca tcatctgttc tgaggttatc
     6541 acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct
     6601 gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg
     6661 tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg gcgctctctg
     6721 tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt
     6781 ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat
     6841 gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag
     6901 ggagatgtga gccagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt
     6961 tatcaagatg taattagatt attattcttt tactgtatct accgttatcg gagtggctat
     7021 gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca
     7081 ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagcgc
     7141 cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcggcac caggtgtgcc
     7201 tttaaaagcg atcaaatgga tgttccagcg ccatgcgccg ctggcggtcc gtctcgacgg
     7261 tacgcagttt cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta
     7321 catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc
     7381 attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt
     7441 ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg
     7501 cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggagcccg cgctggcaga
     7561 agtagcgcac aaactgacgg gtggtctgca actgcccaat gatgaaaccg gagactgcca
     7621 gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccgctttaa
     7681 tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtgtggcga
     7741 tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct
     7801 caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga ctattccaat
     7861 tgcgcaggaa gatggtgcgc cagtatccac tattcttgat gaaacctaca aaatcgccat
     7921 tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac
     7981 cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg
     8041 cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg
     8101 tacgccggtt gtcgggcgta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac
     8161 gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg
     8221 cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc
     8281 atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat
     8341 acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc
     8401 gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg
     8461 tatctggagc gcgctcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat
     8521 aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat ttttccatgc
     8581 tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca gcaactggca
     8641 gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa
     8701 cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg cttaccgtct ggcagcagtt
     8761 gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg cattttgccg aagcggaaca
     8821 tcctgatgga atttccagcg cgatggcgcg tattgagcag gcggcggaag ggctggagtg
     8881 tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc attttgactg
     8941 ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc
     9001 caataccgga ttacgtccgg tgatgactct aagcagtgag attattggtg tccagacgct
     9061 aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg aacagcgaat
     9121 cggcattgtc gccgcagggt acgccgacgg ttatccgcgc cacgcgccta ccggtacccc
     9181 tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg atatgctggc
     9241 ggtcgattta acgccttgcc cgcaggcggg tattggtacg ccggttgagc tgtggggcaa
     9301 ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg agttgatgtg
     9361 cgcgctggcg ttacgcgtcc cggttgtgac agtgtaactt gttgtaagcc ggatcggagg
     9421 cagcgtcttc tggttgcgaa aaaatcatcc atccggctgg tcagcaactg tagttgttaa
     9481 tgtgacagag ccattgccca tgatagtgtc cattaaaagg atggacacta tttccccgga
     9541 acctgaactc accgcacagg cgttctacat aaaacgctta cgcttcattg ttgactcgga
     9601 gtcgacttca gataaatcac gctttaccct tgatggagcc tgtacataga tttgtgtaat
     9661 tgcctgattt tgatatgttc aattcaacat caaatgaagg ttaaattatg gacgacaaac
     9721 aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc ggcgatagat gcttcattaa
     9781 atttaagatt cagcttcctc ttcagccacc cgtacgccaa tcttcaacac ttcattatct
     9841 tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt
     9901 gccgcgccta acaactgctg gacaatttca cccagcgttt gctgcttatc acgatattct
     9961 cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga
    10021 atgaagtcac caaagaagcg ttgatccagc gcgaccggcg gcgactggct gaacagttta
    10081 ccgagcgccg gaagatcgcg ttcccgacca attacacaca acacatcgcc ttcgcgcagt
    10141 cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagtgctgc aatacgcgtc
    10201 tcttttggca tatgcaaatc gcgcagtgcc gcgcccacgc accatttatc ggcactcaat
    10261 tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc
    10321 ggacgtccca ctggcggaac caccactttg gcttttttag ccgcccacga gagtgatgtt
    10381 ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagacgtgca
    10441 ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc gccgcgtaat
    10501 cctacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaggg gagcaatcca
    10561 gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga
    10621 atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg
    10681 atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc
    10741 ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc
    10801 gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg
    10861 gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagtccgatg
    10921 acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt
    10981 tcatgttgct ggatcatcgc aatcagggta atcgtcagaa agaccgccat tggatcatta
    11041 ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc
    11101 agagaaaaga ccgctgcagc atcggtagag ccgacgatag cgccgattaa taagccttca
    11161 atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagc
    11221 acgcccagcg tcgccagcga cagtgccggt cctaacgcca cacgaaagga actggcctga
    11281 gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca ggttacttac catgtaggcg
    11341 aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcctgccag catgccgatc
    11401 gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata
    11461 ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaac
    11521 ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga
    11581 cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct
    11641 aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag
    11701 cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg
    11761 ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt
    11821 gctgccgcta aagctaccga gaatgatcgc cttctgacgt ggcaaaattc ccgcatgata
    11881 gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt ccagcaccag
    11941 aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag
    12001 attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat
    12061 ggtgaaggtt ttattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc
    12121 atccgcgcca aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc
    12181 cagaagaccg cactgaatgg cggtaaaatc gctatgcccg caaatgagca acgggtcatg
    12241 ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta
    12301 accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag
    12361 atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc
    12421 gacgttattg acctgatgcc cctcgtcggt aaggcgctgg ataccacgca gcgcggcgtg
    12481 ctgtttaatg cagtaacccg atggggcaat taagtgaaac agagacatgg caattccttg
    12541 ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg
    12601 cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg
    12661 ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt
    12721 ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc
    12781 gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg
    12841 gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat
    12901 gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgaactgaa aaatccagag
    12961 cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc
    13021 attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt
    13081 gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg
    13141 gatgctgacg agttcctcga acacgtagcg cgaaatcacc ctgcgccgca ggctccgcgc
    13201 tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgt actttgtccg
    13261 ctttctcccg ggcttccggg cttcccgctc gagagagaaa ataatccgtt gtaattgccg
    13321 ctccaccgtc gggctgacgt taagaaaacg gaagctcaga cggggagtgg tgatggtttc
    13381 attcttgcca tcaatcactt tgcgctcgct gatggagatt aactgggcgt caaagtgaaa
    13441 aacaccccat tgccccatgt tgacttcaat ctgagcgaag cgcatgcctt cttgtaattc
    13501 ggcaggcttt gctgtttcca gtaatgcgcc catgccgcct aacgacaaat catacaggcg
    13561 gaaacgtaac gtactgttat ccgccagttt ggtctggcaa aaataaggcg gatggagtgg
    13621 ggcggagatg cggaaatatc ggcgtcgttg tacaaaccat aaggtgggag gcggtacggt
    13681 aataaatgcc ggaagctgca agtattcact ctgctgtagt tgttcaacag taaactcgac
    13741 tttcgcaccc tgagtttcgg cggtaatggt aatgtgctgc gcctttagca cggcgatgtt
    13801 gtcttcggct tgactgccga aatccagcac cagtttatcc ggggttattg ccagtatttt
    13861 gctgatcagc tgcccgccat tccaactgag ccgcaaagga attgcggctt tgtgcaaatc
    13921 gcgtaacacg cccaggacgg ctaacggatt ttgtttcagg aactgctcat ggtaatgact
    13981 cacgcggaaa aactcctgac tgacaaactg tctttggttt atcggtcaca gttaacaaaa
    14041 cttaatacaa atgcgtgaat attttttaca tgttgttctt aaatcagccg acatacgccc
    14101 agcatcgatc ccgcccttgc ctatacttag agcgttgacg taagcatttc ttgcgtccga
    14161 ttcatcgaaa cgagggcctg aacatgggaa ttattgcctg gattattttt ggcctgatag
    14221 ccggcattat cgccaagcta atcatgccgg ggcgtgatgg tggtggattt ttcctgacct
    14281 gtattctcgg gatagtcggt gcggtggtcg gcggctggct ggcgaccatg tttggcattg
    14341 gcggctccat cagtggtttt aatctgcaca gcttcctggt ggcggtggtg ggagctattc
    14401 tcgttctggg catattccgc ctcctgcgaa gagaataaga ttttcataag gcggatagcg
    14461 atacagatgc cgctatccgc tttcacatca gaacgtatac tcgacacctg ctttacgggt
    14521 gaaaaaaatc aatatagcac ttcgatatcc gataaagtcg gttcccggta acaacaacag
    14581 aatccttttt taattattgt ttcgttgttt ttggtgtgat tcagaaaata aatattttag
    14641 acagtagcca gtcaggcgtt aaaaatgggg cggatagtga ggcggttttg gctgcgctta
    14701 ttgaacattt agaattgatc aatccttctg gcagattaag ccaaaatacc cgttcggcaa
    14761 tgcttcagtt acgcgaagag gaatggtctg aattcttttt ttggttgcta aattctctgg
    14821 agtgtctgga ttatgtgatt attaatttaa ctccagaaag caaaaaaacg ttgatgagtg
    14881 aacaccgtaa caatattcag gtagcaattg atgctttata tagccaacgt cggcgtaaat
    14941 cgccgggtga tgaaagtgaa acacttaccc gtagaaatga cgctatcttc ggcaaccacg
    15001 tatggcaaac atttgcgcaa tacttcccgc caggacttga aaaaccgtct gtgtagaaaa
    15061 aaccatagct atggtgttat ctgccgacaa cgccatccgg agaagccgaa tgacgttgtc
    15121 ggagtaagcg ctggttaagg tgtgggttgt gcctctttgg ttgagggttg cgtcgttgct
    15181 gacttaacgg tcggacgcgt cgccggaaca ttgtcacacg gttgctcttt cgggcagatc
    15241 aaatccagca ttttcagcgt cacgccattg gtccagccaa agccatcctg taatggatat
    15301 tcgccaccgc cgccccccgt tccggtggtg ctgacatcat atttttccac cagctttttc
    15361 tcccggtcat aggtgtgctg aacattggtc aggaagtgcc agctaatgtc catcgccacc
    15421 tctttttgcc cgtagttttg taatccttct gtcgcgaccc actgtaacgg tgcccagcca
    15481 tttggcgcat cccattgttg cccacttttc accgacgtgg tgttcaggcc gccgggttgc
    15541 agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc tgccgcattg
    15601 acgtacagcg ggaacagggc ggccgcggtt aactgattgc gcactttatg acttttcagg
    15661 tcgtaatcgg cataccagcc ttgttgatcg ttccacatgt atttttcgat ccctttttga
    15721 cgggcatttg ccagcgtttc gtactggttt gccatcgcgt tatctccggc agctttgctg
    15781 gcgcgggcga ggattttttc cattttaaac atcaggctgt tcagatcgac cggtacgatg
    15841 ctggtggtgc gtaaggtatt tagttgatgc gggttgtcca tccagcgcga gctgaaatcc
    15901 cagccagacg cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga
    15961 ttgcttttgg cggtggcaat atcttccacc catgactctg gtcgtggcgt atcgcgatcg
    16021 tcccagtagc ggttgagaag ggtaccatcc tgaagtttta caacgcgttt ttcctgttgt
    16081 ccggcttgca ggttttcaac accgtccatc cagtaagcat attctttttg catttgcggc
    16141 aggtattgct tcaacgcggc atcgccttca tgctgcgcca gtaactctac catcagggca
    16201 aagaagggcg gttgcgagcg gcttaaatag taactgcggt tgccgttggg aatatgaccg
    16261 taagtgtcta tttcatgagc aaaattggcc accatatccg cgactttatc ccagtgaccg
    16321 ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt
    16381 ccgcccggca cgacataagg ttccggcagc ggtaacagag aatcccattt ttcggtgttt
    16441 tcggtagaac gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag tgactgtccc
    16501 tctggcggaa catatttctc gccctctttc ggcagggtga aattgacgtt aacgaaatgg
    16561 cgcagatcaa atccgctctg gttttgctgc atccgataat cagcaaggat catcagcgga
    16621 tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc
    16681 acatcattaa acagcggccc taataaaata tcaggcggct gtggtgttac cggtgtttct
    16741 tctgcctgca ccgatagcgc agcgaaacac aaaaagatac aggctggaat taacgccatt
    16801 ttttgcgggc gagaaggtgc gggggatttc atcaatcatt ctcctttggc gaaaccgaat
    16861 aaaacggtat tcaagtatca gaaaacctta gttcaggatc gcactagctg catgatcgaa
    16921 atctcattct gcgaactgtc aggcatttta gaaaagggaa ttaaagggat aaataacggc
    16981 gaaatagagt gatcaaataa cgtctgttta ttagccagcc agcggcgttg ggttcgtgat
    17041 tagcgaagaa aaatcatcat aaattggtga aaaatataac aggtagttaa aaaccattag
    17101 tgctgagtaa attgccggat gacatcagaa cgatgccatc cgaacagtgg cttaaccctg
    17161 acggttgaaa cgttgcgttt taacgtccag cgttagcgtt tcttctggtt gtatcgcata
    17221 cagtttctca ccctgctggc aaatccagcc aatccccagc tcacgggcga ttagggcgct
    17281 gtgggatacc ggacttccgg cgctaaggca gatacctttt acaaccgccg gatccagttg
    17341 cagtactgtg gaaggataaa tgttctccgc cagtagaata gttggcgagt taaactgcgg
    17401 gagttcttct ttcgtttggg tcaggtggac cagggtgcga tgcagaagat cgtccacatc
    17461 aatatagcga gcttgtagat attcatcatc cagttgctgg tattgctggc taagttcttt
    17521 aagaacttgc tgccaggcat attctgccgt gcaatgttca tgctgaagga gttcgcttgc
    17581 cgccgccagc agttccggat catctaacag tgtatggtgg ccagaaaaga ttgcggcaat
    17641 atcgtcaagc ccgctggctt ctgctttcgc tgttaacgtc atcagatcta ataacgtgaa
    17701 gtcaatagcc tggcgtaatc gatcttgttc ttcttccacg gtcagggttg attttgcctg
    17761 taccgtacat aaaactggtt gataataaaa ggctttaccc gaaacaggcg gaacgggacg
    17821 cagagtaggt ggagcgactt cctccgtttc accaaagtta tcttcagcca gctgacggaa
    17881 agcgatcagt gcctcttcag cttctggccc tttcgcaatc aggcgcagcg tatcgttata
    17941 gcgaacttgt agtaacgcaa tctggttaat actctctggt gtgacgcatt tgccgttttt
    18001 ttccagcaac atatcggcat taaatgtcga taaggtataa accagccggg aggccggacg
    18061 tacatgcagg ccgttacggt tttttatgac caccgccaga gaacgggctt cttcatcgta
    18121 cgcaggacat gtgtcagaga ttttagtgtc ggaggacggt aaacccagtt gttcacgttt
    18181 ggcttccagc gcatgcatag cgtcaaagat aactttgtcg atatccgctc ccgaggccgc
    18241 gctgaccgtt gctgccagtg taccttcgac caacggcgca gcacacaaac gtacttttgc
    18301 ggcgatcttg ggagccagca attccagcgc agtttcagca ctcaataatg cgctacccat
    18361 atccatcatg accagcacat ggtcggcatc agcaacagat tcgatggcct ccatcacttt
    18421 gacggcatcg gtaccaatag gattttgtgg atcgtcaatt cccgcggcaa tggcgatttt
    18481 acaactatca ctcattaaca tctgacgggc taattcaccg acaccttctc ccagtcggct
    18541 gctatgtgaa actatgacca ggtttaccat caccaattcc ttactctttt gcggctaacg
    18601 ccaacatttg catcataaac atcaccgagg tcgcgccggg atcctggtga ccaatactgc
    18661 gttcaccgag ataactggcg cggcctttgc gggcttgcat cgtaatcgta ctttgtgcag
    18721 cggattcggc gattctactg gcagcttcga gcgccaccgg aacagagaga ttttgctcgc
    18781 tggactgacg taacgattcc accaccggca cccacacatc acacatggtt ttatcgccag
    18841 gttcggcttt cccgcgactg attacgccgt ccgcgccatc gcggaacatc tgataaagct
    18901 cttccagtgt caggctttgc cgtgcctggg tcgcctgtgc ggcgcggata aagaaggtac
    18961 cgaacagcgg accactggca ccaccgacgc tggaaagcag cgtcataccg gtattcttga
    19021 gaatgaaacc gatatcttta tctgcgatag cagggagttt ttccaccact ttgctaaagc
    19081 ctcggttcat atttagcccg tggtcagcgt cgccaatttc gcgatccagt ccggtaagat
    19141 actcgctttc ggtgctgaaa atatcgccac aacgagtgag ccagttaaca atttgagttc
    19201 tgctcagtga cattgctttc tccttattta ccccagttaa gggccggggt gtggaccggg
    19261 gcgtcccaga gtgccagcgt ttcgtcatca actttcagta aggtgattga gaaaccggtc
    19321 atatccagtg aggtgcagta cgcgccaatt aaattacgtt cgatagtcaa tcccgcttgc
    19381 tggcaacgtg tggtcaggcg gttatagacg ccgtacagct cagaaagcgg agttgcgcca
    19441 agattgttaa ccagcgcaat cacccgatcg ccagactgga gcggttgttt ggtttgttgt
    19501 tcttcctgcc aactgccttg ttgataatcc cagaaacgca aagtgcgatg gtatgagcca
    19561 tttaccagca gggtgtcgaa catttcatcg acggtttgat caagggaaga gaaggggcgg
    19621 cggtcaatac ccggctcacc atgaatgccg acgccaaact ccatctcatt atccgccagg
    19681 gtaaaagaag gtttgcccgc ggcaggaacg gtacaggcac cgagagcgat acctattgag
    19741 tggccttgat tattcaactt acgccccagt tccgcacagg cgtccagtga gtcgccacgc
    19801 tccgccgctg cgcctacgag tttttcaatt aatacggtgt tggcaacgcc gcgtcgcccg
    19861 gcagtataaa gactgtcttt taccgcaacg tcgtcatcaa tgaccacagt ggtcactttt
    19921 acgccgctat cgtgcagtaa ctcggtcgct gtttcaaagt taagaatatc gccggtgtaa
    19981 tttttgataa tcatcagtac accttcgccg ccatcaattt gcatggcgca ttcaaagatt
    20041 ttatcgggcg tcggtgaggt gaaaatttcg cccggacagg cccccgaaag catcccctga
    20101 ccgatataac cacagtgcat cggctcgtgt ccgctgccgc cacccgacag cagggcgact
    20161 tttcctgcaa cgggggcatc agctcgggtg acatacaccg gatcctgatg cagtgtcaga
    20221 gatggatgcg ctttcgccag tcctgccagt tgttcgtcca gtacgtcttg cacatcattg
    20281 atcaattttt tcattatttt gctccagcaa ttacggtagg gcatggatga tgttcaacga
    20341 cacggcgacc actgactgcc gatgaatcca ttgtgcatca ggcaagggaa aagaaaatta
    20401 gcccaaatta tgtttcatag tgaaacatat gctttaatga atgttccata ttgaaacttt
    20461 tacgtgtatt aatacttaaa attgcgagcc ggaacacctt ttgtcataag ggatgcggga
    20521 tatgagtggc gcttttaaca acgatggtcg gggcatatct cccttaattg caacctcctg
    20581 ggagcgatgc aataagctga tgaaacggga gacatggaac gtaccacatc aggcccaggg
    20641 cgtgacattt gcttctattt atcggcgtaa gaaagcgatg ctgacgctcg ggcaggctgc
    20701 gctggaagat gcctgggaat atatggcacc gcgagagtgt gcgctgttta tcctcgatga
    20761 aaccgcctgc attctcagcc gtaatggcga tccgcaaacc ttgcagcagc taagtgcact
    20821 tggattccat gacggcacgt attgcgccga gggaattatt ggtacttgtg cgctatcgtt
    20881 agcggctatc tctggtcagg ccgtgaaaac gatggccgat caacatttca aacaggcact
    20941 ctggaactgg gccttttgtg caacgccgtt gtttgacagc aagggccgat tgacgggaac
    21001 aatagcgctt gcgtgtccgg ttgagcaaac taccgcagct gatttgccgt tgacgttggc
    21061 aatcgcccgc gaggtcggaa atttactgct gacggacagt ttgctcgctg aaactaaccg
    21121 tcatttaaat caacttaatg ccctgttaga aagtatggat gatggcgtga ttagctggga
    21181 cgagcagggt aatttgcaat ttattaatgc ccaggcggcg cgggtcttgc gccttgacgc
    21241 gacggcaagt cagggacggg caatcactga actcttaacg ttacccgccg tattgcaaca
    21301 agcaataaaa caggcacatc cgctcaaaca cgtagaagca acctttgaaa gccagcacca
    21361 gtttattgat gcggtgataa cccttaaacc gataatagaa acgcagggaa ccagctttat
    21421 tttattgctt catcctgtgg aacagatgcg gcagttgatg accagtcaat taggaaaagt
    21481 cagccatacc ttcgctcata tgccacagga cgatccgcaa acccgccgct tgattcattt
    21541 tggtcgccag gcggcgcgca gtagctttcc tgtcctgctt tgtggagaag agggcgtggg
    21601 caaggcactg ctaagtcagg caattcataa tgaaagcgag cgtgctgcag gtccttatat
    21661 cgccgtcaat tgtgagttat atggtgatgc ggcactggcg gaagaattta ttggtggcga
    21721 tcgcacggac aatgaaaatg gccgtctgag tcggctggaa ctggcgcacg gcggcacgct
    21781 gtttcttgaa aagattgaat atctggcggt ggagttacag tctgctttgc ttcaggttat
    21841 caagcagggt gttatcacgc gactggatgc gcggcgttta ataccaattg atgtcaaagt
    21901 gattgccaca acgaccgcgg acctcgcaat gctggtggaa caaaatcgtt ttagtcgcca
    21961 gctgtattac gcgttgcatg catttgaaat taccatcccg cctctgcgta tgcggcgtgg
    22021 cagcattccg gcgttggtga ataacaaatt acgcagtctt gaaaaacgct tctctacgcg
    22081 gctgaaaatt gatgacgatg ccctcgctcg cctggtttct tgtgcatggc caggaaacga
    22141 ttttgaactt tacagtgtca tcgagaatct tgctctgagt agtgataatg ggcgcattcg
    22201 cgtcagtgat ttgccggaac atctgtttac cgagcaggcg acagatgatg tcagcgccac
    22261 acgcctttcc accagtctgt catttgcgga agttgaaaaa gaggcaatta ttaacgcagc
    22321 ccaggtcaca ggcggtcgca ttcaggaaat gtcggcttta cttggaatcg gccgcactac
    22381 gctgtggcgg aaaatgaagc aacatggcat tgatgcaggg cagtttaagc gccgggtatg
    22441 aaagacagaa acgatttctg atacatcaga gtgatctata tttcattccg gcgcacgcta
    22501 acatttttca gcatcgttta agggcttgtc tatcccgcac ttaaaagctg aagcgatatc
    22561 ctccgttgac ttgcttctga tcaaagaggt taccctgcgt gtaatccgct tcgaggtaga
    22621 atgtgtgctg tgtgttatac tgtgcactga ctcccacgcc gttattccag ccattacctt
    22681 tgaaactgta cttctcccgg gagttgttca acagatattc tgtatcccct gaaaactcgt
    22741 ggatagcgcc agtcttcaca tacatattca gctggctttt acctgcggtg atgtcatatc
    22801 ccagtatcat gctggcacgc cccagcagcg attcgtagtg attcagatgt atattgagac
    22861 cattactcgc cttcatagcc atctcattct ggtggctgta tgtgagctgg gtttgcggct
    22921 ctatatagaa cccataacca gtaggggtca ggttgaacct ctgcccggct tccagggaga
    22981 tgctcaatcc attcgcagtg ccgttggcgt taacgccgtt gttctgactg tcccgtacgt
    23041 ggaaactatt tttctggcgc gatgctttta caacgagatc gctgtaaaaa ccgttgtgtg
    23101 ccatgtaact ggcgtacatt cccatgtagt ctgaacgtgc ggtaccgtcg ccaccgctat
    23161 agtccggcga tgcatgtgtt gagccaatat acagaccgac atacaacggc attacatcag
    23221 agagacgttt atccccacca aactggatac cgctgtaacc catgtcaaag ccgctcagtt
    23281 tgccactggc gaaggagtcc aggcttcccc cataactgcg cagccagata ttaccgtctt
    23341 tactctgatt tcgcaggtca cccatccgtt gcatcagcgt acggttttca acatagttca
    23401 ataagtaacc aatattgaga taattgccgc cagcatctgc ggtcgtggtg ggtttaggtg
    23461 tgggagtggg ggtaggcgta ggatcgggtt caggcgtagg atcggggttg actatgggag
    23521 gctgagcggg agccggtgtt ggttcaggat taggagtcgg ttccggaact gtcccggaag
    23581 cgtaaagctc ccagttagtg ccatttttac gcacatcgta cagatatccc cccaactcaa
    23641 cctgagaaga cgcgctgaac gaggccgcac cgtcagtggt ttttaccact gtcagaactt
    23701 catttcccgt tgtggcttcg ctgccctggt tacggatagc caatacatga ttaccagcac
    23761 tactcccgct gatattcaat aaatcccctt tattattaac gccattaccc tcgccaacaa
    23821 catcagcacg cataataaag gtactgttac cgctcaggtt ctctacgttt aatgtggtaa
    23881 atgtgccggc agttgatgcg tggctggcaa aatcgacagt tgaatggctc agcgccagcg
    23941 tgtcgagatt agaattactt gttacgttcc agacgctgtt attcattgca acgtccagtt
    24001 tcccgtcatt gacattatcg ctaagggagg aacctgtcca aaccgaccct gggtgcatat
    24061 ccagattgat tagcccacct ttggagagaa cgctaccgtt gataagcata cgacccgaag
    24121 cgttgatgcg gctggcggca taaccatcgt catgttgcgt tgcgatggcc atctggtcgg
    24181 gtgtactcat atcaatgacc agatcgctcg tgaggtcgat ctggctgttg gtcatggcat
    24241 aaatccccct ggctcctgcc gcgccggtga tagccaaact gtctccggtt attctaccgc
    24301 cgctgagcgc ccacaaaccc agcgccagac tgccattacg atcaaccgta atatcggtat
    24361 tttgcatgtt gataacagcc gttgccgtct gggccgaggc accataagaa ccgccggaaa
    24421 agatgctgtt tcgttgcgct gccgtgccag aaaaattgat tgtcgcgtct gaaccactgg
    24481 tgacgaggcc accgccctgc gcggaagaaa tatggctatc tgcaccgata gtggttgtac
    24541 caccacgaac ttcgacgcca ttggccgcgg ctccaattac atcaacagtg agtgcattcg
    24601 cgctaacctg gccaaagctc cagaggccgt gtgcattatc gccactggtt ttaatggaac
    24661 tgtttgttcc gagatcgaca accgagtttt tctgtacgtt tatccccatg gcgctgtagc
    24721 cctgaacatc gattgtcagg tctgtcgccg taaaacgcgc agcaccattg gcgttattgc
    24781 cgttgagacc accgatataa acacctgtac ttccatcggt cttgatttta cttccgcttc
    24841 caagatcgac actggtgcca tagtcattga tggttaggcc tataccgttc gagttttcaa
    24901 tggtgaattg agtggctgtt agtgttgagc tatgcccaat aatgatgccg tcatcgttac
    24961 tcttaatggt gctgcctgtg cctaagtcag catgggtata gtcaccaatt aaattaatgc
    25021 cgatggcaga ggtttgacca acaacatcta ctgtgagtcg gttagctgtc agggagctgt
    25081 ttttgccctg aataatgatt ccttttgcat atccagaggc gtcgttgaca ttgacggtga
    25141 catcattacc caggttgata ttgccaggcg tgtttcctgc tggggtcatt acaccataga
    25201 ggtttcctga tggatcggcg gtatctcctg taattctgtc accatcatta atagtaatat
    25261 tggtgccata ttgacttcct gtatcagcag ctatcagcag gggggaaaag ataattgcat
    25321 tcaaaccaat agcgccaaat tttatcagtt gaattgaggg cgaacggatt tttaattcag
    25381 cgagacgatc ggctttggta ttcccattgt gttgtttgat gcccatacca ctcctatata
    25441 gtacccagct atgggtctat ggattttgtc attttgttta agtcaatgaa taatatcctc
    25501 ttaccatcat gatatttgta tggtgcaatc atgatgagct gctatgagaa taattctcta
    25561 ttaccgggca aaatcagaaa cacaggaatt aatatatatt taacatcccc cttacaaagg
    25621 tgagtttaga tcgagttatt ccctaagttg tcgttactta ttccaattcg ttactatgaa
    25681 tatggcaaaa atattacaac aaacagcggt taaaaaattt ggtgaagatt ttttgtaata
    25741 atcctaatta gttatggggt tttcgcttga tgtatctgca taacaaaaac aatatgcgcc
    25801 atttttgcat tactaataag aaaaagcaaa aaacatcgat attcattttt ggtcaatggg
    25861 ttttctggcc tatcgttgtt attgtgctac tggttatcat tttcatcttg ccaggttcgc
    25921 acgtaatctc attattcggg gactttatct gcgggatatt acccttcagg taagtccttt
    25981 tctcgccagc atattggagc gtgatcaata ttgatcagct gtgaaggggg tgatgtgtag
    26041 tggaacaaaa acttacgtta tatcactact aatgagatat ccgtcatctg aacactgatg
    26101 tctttaatta ttctggtggt tcactgtagg caataagcac aaaagtgtag gatgttacaa
    26161 gaatgattag gactcggtga aatgaaaaat ccacgcaatt gcgtggattt tatatacttt
    26221 tgcgctcttc atgagattta gcgaaacctc atgagacaac aaattaatta gacgttgaaa
    26281 aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc acgcattttg
    26341 cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa cgagatggtt
    26401 tgtgcacgga taaagccttt ttcaaaatca gtatggattt tgcccgctgc ctgcggcgcg
    26461 gttgctccaa ccggaatggt ccatgcacgc acttctttca ccccagcggt gaagtaagtt
    26521 tgcaggttca gcagcttata accggcacgg atcacacggt tcaggcccgg ctcttccagc
    26581 ccaagctcct gcataaactc gtcacgttct tcgtcgtcca gttcggcaat gtctgcttca
    26641 acagcagcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc acgcacctgg
    26701 tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta cattgttggt
    26761 ttcagcgtca ggaagctcag gtaacgaata gccgctttct cttcagcgct taaatccagc
    26821 gcgcgcagca tacctgcgtt ttccagctgg ggcaggcatt tttccaggac cgccagctca
    26881 gctttcgcgt ctttatcgcc acctttggct ttcttctgta cgcgatgaat cgcacgttcg
    26941 caggtgtcga ggtctgccag cgccagttcg gtgttgataa cttcaatatc gtccgccggg
    27001 ttaactttgc cggaaacgtg aatgatgttg tcattttcaa agcagcgaac aacgtgaccg
    27061 atcgcttcgg tttcacggat gttagtcagg aactggttac ccagaccttc gcctttcgat
    27121 gcgcctttta ccagaccggc gatatcgaca aattccatgg tcgtgggaag cgtacgttgc
    27181 ggttttacga tttcagccag ttgatccagg cgagggtcag gcattggtac gacgcctgtg
    27241 ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa taccggcttt ggtcagcgcg
    27301 ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt gaatcccatg
    27361 atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat tgcagaaatg
    27421 gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca acgacttatt
    27481 gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta aaccacattt
    27541 cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca ctaacaggcg
    27601 gtttgcctaa cacaaaaccg acaactttat ttttatcgcc cggatgaccg attccgatgc
    27661 gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc agtccattgt
    27721 gaccaccatg gccaccgccc aatttaaatt tggcgacgcc aggaggcaga tccagttcgt
    27781 cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc gccgcaacgg
    27841 ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct tcgcctccaa
    27901 gagtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac
    27961 gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg gcgtattcag
    28021 caccggggtt cgccaggccg acaatcaatt taatcgtcac gtttttttgt cctgagtgtg
    28081 tacataactg gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa
    28141 atgcagataa cgtaataatt gcatgagtgg actattagaa agtcaaggtg ttcaggcgtt
    28201 tatttgtaaa gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca
    28261 cagggtgttg tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa
    28321 agttccggag gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg
    28381 ggttgggtct ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac
    28441 agtttaatat gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca
    28501 ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt
    28561 gggttcgcca ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa
    28621 taatgcagtt tgctgaccag tcagtttgcg ctgactggtc aatctcgtac ttataaatcc
    28681 gccatcgccg cgcgacgatt cgggaagaac gcgaggcgtc ccgggatcgg ttgaatgcct
    28741 gcgcgagcca tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatcct
    28801 tcgggcagac gcttcacaaa acgctggaac gcatcaagac caccagcatc aagcaccgga
    28861 acggcatccc acttcagaat aacaatccgt tttccttcca gacgtgattc caggtcggtg
    28921 aacagacctt cagccgccgc gaaaaacagc gggccaataa cacgcaagac cagcacatca
    28981 tcaggaacgt ctactgctac cggcgccaga cgagtcatac gggcgatacg gcgcataaac
    29041 agcaacgatg ccagaacaat ccccacgctg atggcaataa ccatatcaaa cagcacggtc
    29101 agcgacatgc acagcagcat gacaatgatg tcatctttcg gtgcatgacg cagcaagtcg
    29161 accactttat gcgcttcact catgttccac gccaccatca acagcagggc agccatggcg
    29221 gaaagcggca gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata
    29281 gagtggatca ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc
    29341 gcgatggctg ctgtagcggt aatgccacca aagaacggag cgatgatatt ccccagtccc
    29401 tgtccaacca gttcgctatt cgccttgtgt ttcgtcccgg tcataccatc cagcaccacg
    29461 gcgcagagca gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt
    29521 gtgcgaatag aatcccaggt tagcgtgaat tctgaattag gcagatccca cggcagcacc
    29581 agttgcggca gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg
    29641 aattgcgaac cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg
    29701 caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccaaaaa
    29761 acaagaatac ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc
    29821 gccataaata atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga
    29881 ccgagaaaat ctttaatctg catggtaccg atggtgatcc cgatacccga ggtgaaacct
    29941 aaggtgacgg aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga
    30001 atcaaaaaga tccccgacag caaggtcgca accagcagtc ctgccagtcc aaactgttgc
    30061 gacacgggat agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac
    30121 ccacccgtca gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt
    30181 gccacaccac taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt
    30241 atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg
    30301 tcgatcagag cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt
    30361 catccgtgag cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa
    30421 atggatatta cagacaaaaa aacccgccgc agcgggtctt tgagccgggt tcgattagtg
    30481 ttcgaacatg gcagagatcg attcttcgtt gctgatacga cgaatcgctt cggccagcat
    30541 acctgacagg gtcagagtac gcacgttcgg cagtgatttg atttcatcgc tcagcggaat
    30601 ggtatcgcag acaacgactt catcaattac agagttacgc aggttgttcg ccgcgttgcc
    30661 agagaagatc gggtgagtcg cgtacgcaaa tacacgttta gcaccacgtt ctttcagagc
    30721 ttcagcagct ttacacagcg taccgccagt gtcgatcata tcatcgacca gtacgcagtc
    30781 acgacctgca acgtcaccga tgatatgcat cacctgggaa acgttcgcac gcggacgacg
    30841 tttgtcgatg attgccatat cggtatcgtt cagcagctta gcgatagcgc gggcacgcac
    30901 aacgccgccg atgtccggag aaaccacaat tgggttatcc agattcagct gcagcatgtc
    30961 ttccagcagg atcgggctac caaatacgtt atcaaccgga acgtcgaaga aaccctgaat
    31021 ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg agaggaagtc
    31081 tgcaaccact ttcgcagtga ttggtacacg agcggaacgg acgcgacggt cctggcgcgc
    31141 atagccaaag taggggataa cagcggtgat acgacctgcg gaagcacgac gcagggcatc
    31201 aaccataacg actaattcca tcaggttgtc gttagtaggg gcacaagtgg actggatgat
    31261 gaaaatatca ccaccgcgta cattttcatt aatttgtacg ctgacttcgc catcgctaaa
    31321 gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag
    31381 ttccggggtg gcgttaccag caaaaagctt catatcaggc acgagaagaa cctcaggcat
    31441 gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga tcctttccag
    31501 gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc accgaaactc
    31561 agcttgcccg gcttaaagca tggctctgtg caatggggaa agattaacgc ctttcgccac
    31621 aaagccattg agccattccg gggcttgctc tagcacctgg cgggcttcag actctgtatc
    31681 aaattcagca aagacacagg cccctgtccc agtcaggcgc gacggggcgt attctaacag
    31741 ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt gcgataacct cgcaatcatt
    31801 gctgaattca cattttagca acgtttctat tgaccttttt ggcgtattgc gcgggagttc
    31861 aggatcttta aaaatcaccg gagtcggaat acttacacca gggtgcgcca ccagatacca
    31921 cttctctggc ggatccaccg gcgttagtat ttcaccaacg ccttcggcaa acgcggcatg
    31981 cccccgaaca aagacaggaa catctgcgcc cagcgtcagc cccatttccg ccagctcatc
    32041 catgcttagc ccgcattgcc agagatgatt taatgccacc aggaccgtcg cggcattgga
    32101 tgaaccaccg ccgagaccgc cgcccatcgg caaacgcttg tcaatgctga tattcgcacc
    32161 gcttcccgtc ggaagacgcc cgctgtctgc cgcagttttt atcaacaatc gcgctgcgcg
    32221 aacgatcagg ttatcttcat gttccacgcc ttcaacgggc gttaacagac gaatatcccc
    32281 atcgtcacga agctcaatgc tgatggtgtc gccgtaatca agaaactgaa acagcgtttg
    32341 cagcgtgtgg taaccatccg cacgctgacc ggtaatgtat aaaaacagat taagttttgc
    32401 cggagagggc cactgtgtcc gcattatttc actatccagt tatccatttt taacttgatg
    32461 cgttgaccac cgtcggtgag ttccatattg gctggcatcg caggttgcgt tttggtgtca
    32521 taaccaccat aaacaacctt ccagtttttg ccattctggc tgtaggtaat ttcgctcagg
    32581 cggtactggt cgtccagttt gtagtcggtt gcatcacccg gtaaacctaa aatccactgg
    32641 cgcaagctgt tgagcggaat tggcattccg gtcaatttgc caatcatctc ttcggcgtca
    32701 tcggcggtat aacgctgacc tttattgtcg actaactgca cgttacccgg ttgagcattc
    32761 agctccagtt ccgtgctgcc caatgggtta gtgagcagca gacggtagcg atcctggccg
    32821 gtttgctgcc agaaaaagcg ggcgtacact ttttgttggt cagaaatata agcgaacgcg
    32881 ccgcgagtct gatactgatt aagattgcgc acgtcttgct gatgctgacg ccattgtggc
    32941 gaatccgggc ttttgccagg acctttgggc gtggtaacgg aacaggcagt aagcacaaga
    33001 gcagccagcg gtagcaggcg gataagacga aaatcgggca ggggcatagt gatgacaagt
    33061 ccttgagata cgttgcagtt ataaccctta atgctagcgt taccgtccgc tatcgtctat
    33121 gttcaagttg tcttaattgc cagaatctaa cggctttcgg caattactcc aaaagggggc
    33181 gctctctttt attgatctta cgcatcctgt atgatgcaag cagactaacc ctatcaacgt
    33241 tggtattatt tcccgcagac atgacccttt tagcactcgg tatcaaccat aaaacggcac
    33301 ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa gctcgatcag gcgcttgaca
    33361 gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct gtcgacgtgc aaccgcacgg
    33421 aactttatct tagcgttgaa gagcaggata acctgcaaga ggcgttaatc cgctggcttt
    33481 gcgattatca caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata
    33541 acgacgcggt tagccattta atgcgtgttg ccagcggcct ggattcactg gttcttgggg
    33601 agccgcagat cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggtcatatga
    33661 aggccagcga actggaacgc atgttccaga aatctttctc tgtcgcgaaa cgcgttcgca
    33721 ctgaaacaga tatcggtgcc agtgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc
    33781 ggcagatctt tgaatcgctc tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca
    33841 tcgagctggt agcgcgtcat ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc
    33901 gcactcgcga acgtgcccaa attctggcag atgaagttgg cgcggaagtg attgccctga
    33961 gtgagatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt
    34021 taccgattat cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa
    34081 tgctgttggt ggatattgcc gttccgcgcg atgttgagcc ggaagttggc aaactggcga
    34141 atgcttatct ttatagtgtg gacgatctgc aaagcatcat ttcgcacaac caggcgcagc
    34201 gtaaagccgc agcggttgag gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg
    34261 cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc caggcagagc
    34321 acgttcgcga tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc
    34381 aagccattat gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga
    34441 aatcacttca acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca
    34501 gcctcgggct ggagtagcag tacatcattt tcttttttta cagggtgcat ttacgcctat
    34561 gaagccttct atcgttgcca aactggaagc cctgcatgaa cgccatgaag aagttcaggc
    34621 gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga
    34681 atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga
    34741 tatcgaaacc gcacagatga tgctcgatga tcctgaaatg cgtgagatgg cgcaggatga
    34801 actgcgcgaa gctaaagaaa aaagcgagca actggaacag caattacagg ttctgttact
    34861 gccaaaagat cctgatgacg aacgtaacgc cttcctcgaa gtccgagccg gaaccggcgg
    34921 cgacgaagcg gcgctgttcg caggcgatct gttccgtatg tacagccgtt acgccgaagc
    34981 ccgccgctgg cgggtagaaa tcatgagcgc cagcgagggt gaacatggtg gttataaaga
    35041 gatcatcgcc aaaattagcg gtgatggtgt gtatggtcgt ctgaaatttg aatctggcgg
    35101 tcatcgcgtg cagcgtgttc ctgctacgga atcgcagggt cgtattcata cttctgcttg
    35161 taccgttgcg gtaatgccag aactgcctga cgcagaactg ccggacatca acccagcaga
    35221 tttacgcatt gatactttcc gctcgtcagg ggcgggtggt cagcacgtta acaccaccga
    35281 ttcggcaatt cgtattactc acttgccgac cgggattgtt gttgaatgtc aggatgaacg
    35341 ttcacaacat aaaaacaaag cgaaggcact ttctgtactt ggtgctcgta tccacgctgc
    35401 agaaatggca aaacggcaac aggccgaagc gtctacccgt cgtaacctgc tggggagtgg
    35461 cgatcgcagc gaccgtaacc gtacttacaa cttcccgcag gggcgcgtta ccgatcaccg
    35521 catcaacctg acactctacc gtctggatga agtgatggaa ggtaagctgg atatgctgat
    35581 tgaaccgatt atccaggaac atcaggccga ccaactggcg gcgttgtccg agcaggaata
    35641 atggaatatc aatactggtt acgtgaagca ataagccaac ttcaggcgag cgaaagccca
    35701 cgccgtgacg ctgaaatctt attggagtat gtcactggca gggggcggac ttttattctg
    35761 gcctttggtg aaacgcagct gactgacgaa caatgtcagc aacttgatgc gctactgaca
    35821 cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg
    35881 ccgttatttg tttcgccagc gaccttaatt ccgcgcccgg atacggagtg tctggtggag
    35941 caggcactgg cgcggttgcc tgaacaacct tgccgtattc tcgatctcgg gacgggtacc
    36001 ggggcgattg cgcttgcgct ggctagcgag cgcccggact gcgaaattat cgctgtagat
    36061 cgtatgcctg atgctgtctc cctggcacaa cgtaatgccc agcatctggc gatcaaaaat
    36121 atccacattc tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt tgcgatgatt
    36181 gtcagcaatc cgccgtatat tgacgagcag gacccacatc ttcaacaagg cgatgtccgc
    36241 tttgagccgc tcactgcgct ggttgcggca gacagtggaa tggcagacat cgtgcatatc
    36301 atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga acatggctgg
    36361 cagcagggcg aagcggtgcg acaagcattt atcctcgcgg ggtatcatga cgtcgaaacc
    36421 tgccgtgact atggtgataa cgagcgcgta acgctcggcc gctattatca atgacaagtt
    36481 tttctacact gcttagtgtt catcttatta gtatcgcgct ttctgttggg ctattaacct
    36541 tacgtttctg gctacgttat cagaagcatc cacaggcatt tgctcgctgg acgcgcattg
    36601 tgccgccggt tgtcgatacg gtgttattgt taagcggcat tgcgttgatg gctaaagcgc
    36661 acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt ggagttatca
    36721 tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt cagcaggcgc
    36781 gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa ctcgccacca
    36841 caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa tttaataaag
    36901 cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat tttccctcgc
    36961 aagatgtcta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa atcagccagc
    37021 ttctgccttt agaagagcag ctggaaaaac tgatcgcgct gttttacggc gactggggat
    37081 ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac caggtgttaa
    37141 agaatcgaca gggcagtgcg gtatcattag gtgcggtttt attatgggtc gcgaatcgtc
    37201 tcgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc attgaatgtc
    37261 cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagc gaacatatgc
    37321 tggacgtatg gttaaaggga aatatcagcc cgtcggcgga actgttttat gaagaccttg
    37381 atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa gcctcgttga
    37441 tggaagaaaa tcagatggag ctggcgttac gcaccagcga agctttatta caattcaacc
    37501 ctgaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac
    37561 acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac ccgatcagcg
    37621 aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg cattaattaa
    37681 tcgacatttt actcaagatt aaggcgatcc tatgaaacaa aaagtggtta gcattggcga
    37741 catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg tgctggaatc
    37801 tcgcgatctg gcgatgcgca tttgcgagca ctacgtaact gtgacccaga aactgggtat
    37861 cccttacgtg ttcaaagcct cttttgacaa agccaaccgc tcctccatcc actcttatcg
    37921 tggaccgggc ctggaagaag ggatgaaaat cttccaggag ttgaagcaga cttttggcgt
    37981 gaaaattatc accgacgttc acgaaccaag tcaggcacag cccgttgctg atgtcgtgga
    38041 tgtgattcag ttgccggcgt ttcttgctcg ccagactgac ctggttgaag ccatggcgaa
    38101 aaccggtgcg gtaattaacg tcaagaaacc acagtttgtc agcccgggac agatgggtaa
    38161 tatcgttgat aaattcaaag aaggtggcaa cgaaaaagtg attctttgcg atcgtggtgc
    38221 taacttcggc tatgacaacc tggttgtcga tatgctgggc ttcagcatta tgaagaaagt
    38281 gtctggtaac tcgccggtga ttttcgacgt gacccacgca ctgcaatgcc gcgatccgtt
    38341 tggcgcagct tccggtggtc gtcgtgctca ggtggctgag ctggcacgag ccggtatggc
    38401 ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccggaacatg cgaaatgtga
    38461 tggtccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat
    38521 tgatgatctg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt ttttgcttga
    38581 aaaataaagt cttagcgttc tgcgttaaga cttttttcat gggtgccaaa tacaaaaaag
    38641 gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    38701 cgcccgtttt caccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    38761 cccgccagga tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    38821 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttctggttca
    38881 agattagccc ccgttctgtt gtcaggttaa acctctcaac gtgcgggggt tttctctttc
    38941 cagcaaccaa tgccaccagg gataaagccc ccgcaacatt gcgcctcacc ggataacgcc
    39001 ggcttgatgt ggatactacg tctcaattca tcttcacttc atccctgaaa tgtttgcaat
    39061 gaagagtgca ttccggtttt tcaacagctg ttacagtcgt ttcatgaggg ctctggatga
    39121 tgcttccagc tcgggttgcc aatatttact tgtggaagag ataaagacaa aaatggccgc
    39181 aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag cctgacgccc
    39241 gttttcacct tacttccggt tacgccacca gccgacaatc gctgcggtaa taatccccgc
    39301 caggatcggt gctgccaggt cgtgccagaa aatcatggca aacttcgcga gcgtcatata
    39361 gccgccttgt tgtaatgaca atattttgcg gctattcttg aattgttgat ggttcaagat
    39421 tagcccccgt tctgttgtca ggttttacct ctcaacgtgc gggggttttc tctttccagc
    39481 aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat aattccggct
    39541 tggtgtggat actacgtctc aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag
    39601 agtgcattcc ggtttttcaa cagctgttac agtcgtttca tgagtactct ggatgatgct
    39661 tccagctcgg gttgccaata tttacttgtg gaagagataa agacaaaaat ggccgcaggc
    39721 tgttacccct gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt
    39781 tcaccttact tccggttacg ccaccagctg acaatcgctg cggtaataat ccccgccagg
    39841 atcggtgctg ccaggtcgtg ccagaaagtc atggcaaact gcgcgagcgt catatagccg
    39901 ccttgttgta atgacaacat tttgcggcta ttcttgaagt gtctggtttc aagattagcc
    39961 cccgttttgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt ccagcaacca
    40021 atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataattc cggcttggtg
    40081 tggatactac gtctcaattc atcttcactt catccctgaa atgtttgcaa taaagagtac
    40141 attccggctt ttcaacagct gttgcagtgg tttcgtgcag gttgtggtac aggctcgcaa
    40201 ttctgattaa cgagctgtcc aggtagtatg aagaaataca ataagcagga atttatctta
    40261 tctcccccga tttccgtcat cggaggagat ataactgtca ggcaaatatc gtcatcaaat
    40321 aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac gttagtacgt ccggtggaga
    40381 aggagatatg gcacagcact aaagaggcca ccatcaccac catttctggc gcaccaagtg
    40441 caaactgtaa ttcgttaccc gtcataaagg caattagcgt gacgacaggt acggtaagcg
    40501 aaatggttgc taacactgaa ccaaagaaca gattcatcgc gcgctggacc tggttgttca
    40561 acactgcttt taacgcacct aaaccttccg gcgacagaat caacagagcc accaggaagc
    40621 cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt ctccagcggg ctggcattca
    40681 ttttggtgac tgcaataacg gcaatcagat ggataatcaa ccagatagca tgccacacgc
    40741 tgctatgggc agacggttta ccgtgatgcg ggtcatcgtc atcactgtca tcttcgtgct
    40801 cgtagacaaa caaactttga tgcgttttgg tctggatcag caaaaatacg ccatacattg
    40861 ccgcagaaat taatgctacc agtaacgcct gaccggttga aaaattcgcc gcaggcagag
    40921 ccattggaaa taccagtacg attatcgcca gggggaacag cgcaattaaa tactgcttga
    40981 taccaaacag attcatatat tgggtggcaa acttacgacc gcccaacaat aatgaaaagc
    41041 caaccagccc accggtaaca atcataatga ttgaatagag cgtatcacgc attagcgttg
    41101 gcgcggcgtc gccggttgcc attaaagctg aaatcaaact gacttcaaga ataaccactg
    41161 aaaggctaag aataagcgaa ccgtaaggtt ctcccaggcg atgggctaat acgtccgcat
    41221 gacggacaac actaaaggcg ctacttaaaa taccaataag cgcaagaaga ttgatggcaa
    41281 tgaccactgg tagtgtctgg ctgcttcccc acaggaacag cactaccagc gccagaaccg
    41341 ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc ctcttgagca tttgacatta
    41401 tggttatccc tttgcagatg aatttatcga aaatgtaaaa aataggtagg aaaaataaca
    41461 gaaagtgtct ggatatcggt aacattttac gaatttttac cccactgtcg atttttt
//
LOCUS       DAZFFI010000031        40897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFFI010000031 DAZFFI010000000
VERSION     DAZFFI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.031"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..868)
                     /gene="fdhD"
                     /locus_tag="ACHJ12_001962"
     CDS             complement(35..868)
                     /gene="fdhD"
                     /locus_tag="ACHJ12_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4989753.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1062..4112
                     /gene="fdnG"
                     /locus_tag="ACHJ12_001963"
     CDS             1062..4112
                     /gene="fdnG"
                     /locus_tag="ACHJ12_001963"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1647..1649,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4989754.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4125..5027
                     /gene="fdoH"
                     /locus_tag="ACHJ12_001964"
     CDS             4125..5027
                     /gene="fdoH"
                     /locus_tag="ACHJ12_001964"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4989755.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5024..5659
                     /gene="fdoI"
                     /locus_tag="ACHJ12_001965"
     CDS             5024..5659
                     /gene="fdoI"
                     /locus_tag="ACHJ12_001965"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4989756.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5656..6585
                     /gene="fdhE"
                     /locus_tag="ACHJ12_001966"
     CDS             5656..6585
                     /gene="fdhE"
                     /locus_tag="ACHJ12_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4989757.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6915..7157)
                     /gene="yiiF"
                     /locus_tag="ACHJ12_001967"
     CDS             complement(6915..7157)
                     /gene="yiiF"
                     /locus_tag="ACHJ12_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO4989758.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7376..7594)
                     /gene="yiiE"
                     /locus_tag="ACHJ12_001968"
     CDS             complement(7376..7594)
                     /gene="yiiE"
                     /locus_tag="ACHJ12_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4989759.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8180..8470)
                     /gene="nadS"
                     /locus_tag="ACHJ12_001969"
     CDS             complement(8180..8470)
                     /gene="nadS"
                     /locus_tag="ACHJ12_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4989760.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8471..8782)
                     /locus_tag="ACHJ12_001970"
     CDS             complement(8471..8782)
                     /locus_tag="ACHJ12_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4989761.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            9011..9919
                     /locus_tag="ACHJ12_001971"
     CDS             9011..9919
                     /locus_tag="ACHJ12_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4989762.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(9983..10924)
                     /gene="fabY"
                     /locus_tag="ACHJ12_001972"
                     /gene_synonym="yiiD"
     CDS             complement(9983..10924)
                     /gene="fabY"
                     /locus_tag="ACHJ12_001972"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4989763.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10969..11406)
                     /gene="dtd"
                     /locus_tag="ACHJ12_001973"
     CDS             complement(10969..11406)
                     /gene="dtd"
                     /locus_tag="ACHJ12_001973"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4989764.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11403..12275)
                     /gene="yihY"
                     /locus_tag="ACHJ12_001974"
     CDS             complement(11403..12275)
                     /gene="yihY"
                     /locus_tag="ACHJ12_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4989765.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12269..12868)
                     /gene="yihX"
                     /locus_tag="ACHJ12_001975"
     CDS             complement(12269..12868)
                     /gene="yihX"
                     /locus_tag="ACHJ12_001975"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4989766.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13009..13896)
                     /locus_tag="ACHJ12_001976"
     CDS             complement(13009..13896)
                     /locus_tag="ACHJ12_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4989767.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(13886..15235)
                     /locus_tag="ACHJ12_001977"
     CDS             complement(13886..15235)
                     /locus_tag="ACHJ12_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989768.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(15334..16767)
                     /locus_tag="ACHJ12_001978"
     CDS             complement(15334..16767)
                     /locus_tag="ACHJ12_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989769.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(16837..17847)
                     /locus_tag="ACHJ12_001979"
     CDS             complement(16837..17847)
                     /locus_tag="ACHJ12_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO4989770.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(17880..18767)
                     /locus_tag="ACHJ12_001980"
     CDS             complement(17880..18767)
                     /locus_tag="ACHJ12_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO4989771.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18792..19670)
                     /gene="yihU"
                     /locus_tag="ACHJ12_001981"
     CDS             complement(18792..19670)
                     /gene="yihU"
                     /locus_tag="ACHJ12_001981"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4989772.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19843..20739
                     /locus_tag="ACHJ12_001982"
     CDS             19843..20739
                     /locus_tag="ACHJ12_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4989773.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20779..21567
                     /locus_tag="ACHJ12_001983"
     CDS             20779..21567
                     /locus_tag="ACHJ12_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4989774.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21678..22943)
                     /gene="yihN"
                     /locus_tag="ACHJ12_001984"
     CDS             complement(21678..22943)
                     /gene="yihN"
                     /locus_tag="ACHJ12_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989775.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFGLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23045..24025)
                     /gene="yihM"
                     /locus_tag="ACHJ12_001985"
     CDS             complement(23045..24025)
                     /gene="yihM"
                     /locus_tag="ACHJ12_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO4989776.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(24033..24743)
                     /gene="yihL"
                     /locus_tag="ACHJ12_001986"
     CDS             complement(24033..24743)
                     /gene="yihL"
                     /locus_tag="ACHJ12_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4989777.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGLTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(24960..26783)
                     /gene="typA"
                     /locus_tag="ACHJ12_001987"
     CDS             complement(24960..26783)
                     /gene="typA"
                     /locus_tag="ACHJ12_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4989778.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            27156..28565
                     /gene="glnA"
                     /locus_tag="ACHJ12_001988"
     CDS             27156..28565
                     /gene="glnA"
                     /locus_tag="ACHJ12_001988"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4989779.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28739..29788
                     /gene="glnL"
                     /locus_tag="ACHJ12_001989"
     CDS             28739..29788
                     /gene="glnL"
                     /locus_tag="ACHJ12_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4989780.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29800..31209
                     /gene="glnG"
                     /locus_tag="ACHJ12_001990"
     CDS             29800..31209
                     /gene="glnG"
                     /locus_tag="ACHJ12_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4989781.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            31321..31431
                     /gene="yshB"
                     /locus_tag="ACHJ12_001991"
     CDS             31321..31431
                     /gene="yshB"
                     /locus_tag="ACHJ12_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4989782.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(31621..32994)
                     /gene="hemN"
                     /locus_tag="ACHJ12_001992"
     CDS             complement(31621..32994)
                     /gene="hemN"
                     /locus_tag="ACHJ12_001992"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4989783.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33183..33692)
                     /gene="yihI"
                     /locus_tag="ACHJ12_001993"
     CDS             complement(33183..33692)
                     /gene="yihI"
                     /locus_tag="ACHJ12_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4989784.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(33811..33977)
                     /locus_tag="ACHJ12_001994"
                     /pseudo
     CDS             complement(33811..33977)
                     /locus_tag="ACHJ12_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34274..34906
                     /gene="yihA"
                     /locus_tag="ACHJ12_001995"
     CDS             34274..34906
                     /gene="yihA"
                     /locus_tag="ACHJ12_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4989785.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35288..38074)
                     /gene="polA"
                     /locus_tag="ACHJ12_001996"
     CDS             complement(35288..38074)
                     /gene="polA"
                     /locus_tag="ACHJ12_001996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4989786.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            38438..39370
                     /gene="yihG"
                     /locus_tag="ACHJ12_001997"
     CDS             38438..39370
                     /gene="yihG"
                     /locus_tag="ACHJ12_001997"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO4989787.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(39411..40841)
                     /gene="yihF"
                     /locus_tag="ACHJ12_001998"
     CDS             complement(39411..40841)
                     /gene="yihF"
                     /locus_tag="ACHJ12_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4989788.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10242 a        10569 c         9917 g        10169 t
ORIGIN      
        1 tgcaatttaa tatatatcgc aggacttatt ttaattaatt gctcaaacgc tgcggatggg
       61 tataaaccgt tgcccgaccc ggcttacaaa aacccaccag cgtcagatta caacgctcag
      121 ccacttctac agcaagcgtg gtcgcggcag acaccgcaaa caaaatctct acgccgcaca
      181 tcgccgactt ttgcaccatt tcataactgg cacgactgga aaccagtacc gcaccttgct
      241 gccagctttc cccttcttgt gaccggcggc ctaacagttt gtccagcgcc acatggcgac
      301 ccacgtcttc atgcccgccg accagttcgc cagatggcaa catccaggca gcggcgtgag
      361 tacaaccagt cagttgcccc actggctgaa aatcgttgag atgacgtaat gcatcatcca
      421 gtttgttgag atcaaacgtc tgggtgaacg gtagcggctg caccggtttt ccgatgtcat
      481 taagttgctc cacgccgcat acgccacagc ccgtacgtcc cgccagcgcc cggcggcgct
      541 ccttcaaccc cataaagcgg cggctggaaa gctcaatttg tacttcaaga ccattacagg
      601 aaggaacgac atccatgccg aagatatcgc gcggactttc tattatcccc tcggaaagcg
      661 aaaaaccgag cgcaaagtac tcaaggtctt tgggcgacgc catcatcacc acatgcgaaa
      721 tgccgttgta gaccagcgca acgggaactt cttccgcgac ctcatctaac cgtgggtgtt
      781 gtaaatcatc acgccgccat aactcaattt gacgaacacc tgtgatattt gtcacatttt
      841 cgatttcttt ttgctgtgtt tttttcactt tctcttaacc agataagaac acacacacca
      901 acattatggt attctgttac aaacccttcc tggatggagg gaaattgagc caattctgga
      961 cctttgcggc cccttccgca aagaaaaata actcccactc cctgcacacg cagcaagcga
     1021 atgtaaatgg gacgtgacaa tgtcgaaaca aggagcaatc catgcaggtc agcagaaggc
     1081 agttctttaa gatctgcgct ggcggtatgg caggcaccac ggcagcggca ctgggttttg
     1141 cacccagcgt agcgctcgcg gaaacccggc agtataaact gctgcgcacc cgcgaaaccc
     1201 gtaatacctg cacctattgt tccgtaggct gtgggctgtt gatgtacagc ctcggtgacg
     1261 gagcaaaaaa cgccaaagca tctatcttcc atatcgaagg cgatccggat cacccggtca
     1321 accgcggtgc actttgtccg aaaggcgctg gcctggtgga tttcatccac tctgaaagcc
     1381 gtctgaagtt tccggaatac cgtgcgccag gttctgataa atggcagcaa atcagttggg
     1441 aagaggcgtt tgatcgcatc gccaaactga tgaaagaaga ccgcgatgct aactacattg
     1501 cgcaaaacgc cgaaggcgtg actgttaacc gctggctctc caccgggatg ctgtgtgctt
     1561 ccgcgtcgag taacgaaacc ggctatttaa cgcaaaaatt ctcccgcgcg ctgggtatgc
     1621 tcgcggtcga caaccaggcg cgtgtctgac acggaccaac ggtagcaagt cttgctccaa
     1681 catttggtcg cggtgcgatg accaaccact gggtcgacat caagaacgcc aacctcgtcg
     1741 tggtgatggg cggtaacgcc gctgaagctc acccggtcgg gttccgctgg gcgatggaag
     1801 ccaaaattca caacggcgcg aagctgattg tgatcgatcc tcgctttacg cgtacggcgg
     1861 cggtagctga ctactatgcc cctattcgtt ccggtactga cattgctttc ctgtcaggcg
     1921 tattgctgta cctgctgaac aatgaaaaat tcaaccgcga atacaccgaa gcctatacca
     1981 acgccagcct gatagtgcgt gaggattacg gctttgaaga tggcctgttc accggctacg
     2041 acgcggaaaa acgcaagtac gataaatcct cctggactta tgaactggac gaaaacggct
     2101 tcgccaaacg cgataccacg ctgcaacatc cgcgctgcgt gtggaacttg ctgaaacagc
     2161 acgtttcccg ctacacgcca gatgtggttg aaaacatctg tggtacgcca aaagacgcgt
     2221 tcctgaaagt ctgcgaatac atcgcagaaa ccagtgctca cgataaaact gcctcgttcc
     2281 tgtatgccct cggctggacg caacactccg ttggtgcgca aaacattcgt acgatggcga
     2341 tgatccagct gctgctcggc aatatgggga tggccggcgg cggcgttaac gccctgcgcg
     2401 gtcactccaa tattcagggg ctgacggacc tggggctact gtcgcagagt ctgccaggtt
     2461 acatgacgct gccaagcgag aagcagaccg atctgcaaac ctaccttacc gccaacacgc
     2521 caaaaccgct gctggaaggc caggtaaact actggggcaa ctacccgaaa ttcttcgtct
     2581 ctatgatgaa ggccttcttt ggtgataaag cgacggcgga aaatagctgg ggctttgact
     2641 ggttgccgaa gtgggataaa ggctacgacg tcctgcagta cttcgagatg atgaaagagg
     2701 gcaaggtcaa tggctatatc tgccagggct ttaaccctgt tgcctcattc ccgaacaaaa
     2761 acaaagtgat cggttgtctg tcgaaactga agttcctcgt caccatcgac ccactgaaca
     2821 ctgaaacctc taacttctgg cagaaccacg gtgagctgaa cgaagttgac tcgtcgaaga
     2881 tccagaccga agtgttccgt ctgccatcga cctgcttcgc agaagagaac ggttcaatcg
     2941 ttaactcagg tcgctggttg cagtggcact ggaaaggtgc ggacgccccg gggattgcgc
     3001 tgactgatgg cgagatcctc tccggtatct tcctgcgctt gcgcaagatg tatgccgaac
     3061 agggtggcgc gaacccggac caggtgctga acatgacctg gaactacgcc attccgcatg
     3121 agccgaagtc ggaagaagta gcgatggaga gcaacggtaa ggcgctggcc gatattaccg
     3181 atccggcaac cggtgcggtt atcgtcaaga aaggccaaca acttagctcg ttcgcccaat
     3241 tgcgcgatga cggtacaacc tcctgtggct gctggatttt cgccggtagc tggacgccgg
     3301 aaggcaacca gatggcgcgt cgtgataacg ccgatccgtc tggcctcggt aacacgctgg
     3361 gctgggcatg ggcatggccg cttaaccgcc gcattctgta taaccgcgcc tccgcagatc
     3421 cgcagggtaa cccgtgggat ccgaagcgtc agttgctgaa atgggacggc actaagtgga
     3481 ccggctggga tattccggac tacagcgcag cgcctccggg tagcggcgtc gggccgttta
     3541 tcatgcagca ggaaggcatg gggcgtctgt ttgccctcga taagatggcg gaaggtccgt
     3601 tcccggaaca ctacgagccg tttgaaacgc cgctgggaac taacccgctg catccaaacg
     3661 ttatctcgaa cccggctgca cgtatcttta aagacgacgc cgaagcattg ggtaaagccg
     3721 ataagttccc gtatgtcgga accacctatc gtctgaccga gcacttccac tactggacca
     3781 aacacgcgct gttgaacgcg attttgcaac cagagcagtt tgtggaaatc ggggagtcgc
     3841 tggcgaataa acttggcatt gcccagggcg ataccgtgaa agtctcctcc aaccgtggct
     3901 atatcaaagc caaagcggtg gtgaccaaac gtattcgtac gctgaaagcg aacggcaaag
     3961 atatcgatac catcggtatt ccgattcact ggggttatga aggcgtagct aaaaaaggct
     4021 ttattgccaa tacgttaacg ccattcgttg gtgatgcgaa cacgcagacg ccggagttta
     4081 agtccttcct tgtgaatgtg gaaaaggtgt aacggagacg acatatggct tatcaatctc
     4141 aagatatcat tcgtcgttcc gcgactaacg gtctgacccc cgcgcctcag gcgcgggact
     4201 tccaggaaga agtggcgaaa ctcatcgacg tcaccacctg tatcggctgt aaagcctgtc
     4261 aggtggcgtg ttcagagtgg aacgacattc gcgataccgt cggcaataac attggggtgt
     4321 acgacaaccc caatgattta agcgccaaat cgtggacggt aatgcgcttc tcggaagtgg
     4381 agcagaacga caaactggaa tggctgatcc gcaaagacgg ctgtatgcac tgttccgatc
     4441 caggctgcct gaaagcgtgc ccggcggaag gtgcaattat tcagtatgcc aacggtatcg
     4501 tcgacttcca gtccgagcag tgcattggct gcggttattg cattgcgggc tgtccgttcg
     4561 acattccgcg cctcaacccg gaagacaacc gcgtctacaa atgtacgctg tgcgttgacc
     4621 gcgtggtggt tgggcaagaa ccagcctgcg tgaagacctg cccaacgggc gcgattcact
     4681 tcggtacgaa agagtcgatg aaaacgctgg cgagcgagcg cgttgctgag ctgaaaaccc
     4741 gcggttacga caatgcgggt ctgtacgatc cggcaggcgt cggtggtaca cacgtcatgt
     4801 acgtgctgca ccatgctgac aagccaaatc tgtatcatgg cttgccggag aacccggaaa
     4861 tcagcgaaac cgtgaaattc tggaaaggca tctggaaacc gctcgcggct gttggctttg
     4921 cggctacctt tgcggccagt atcttccact acgtgggtgt cggtccgaac cgtgcggatg
     4981 aggaagagaa taatctgcac gaagagaaag acgaggagcg caaatgaaac gacgtgacac
     5041 catcgtgcgc tacaccgcgc cggaacgtat caaccactgg atcaccgcct tctgcttcat
     5101 cctggcggcg gtgagcgggc tgggcttttt gttcccgtcc ttcaactggt tgatgcaaat
     5161 catgggcaca ccgcagctgg cgcgaattct gcacccgttt gtcggcgtgg ttatgtttgc
     5221 ctcgttcatc atcatgtttt tccgttactg gcatcacaac ctaatcaatc gggatgatat
     5281 cttttgggcg aagaatattc gtaagatcgt cgtcaacgag gaagtaggtg acaccgggcg
     5341 ttataacttc ggtcagaaat gcgttttctg ggcggcgatt attttcctgg ttctgctgct
     5401 ggtgagcggc gtgattatct ggcgtcctta ttttgcgcct gctttctcaa tcccggtgat
     5461 ccgattcgcg ttaatgctgc attcatttgc cgcagtggcg ttaattgtgg ttatcatggt
     5521 gcatatctac gccgcccttt gggtgaaagg cacgattacc gccatggtgg aaggatgggt
     5581 taccagcgcc tgggcgaaga aacatcaccc gcgctggtac cgtgaggtcc gcaagacaac
     5641 ggaaaagaaa gctgaatgag tattcgcata atcccgcaag atgagctggg ttcgagcgag
     5701 aaacgtacgg cggatatgat tccgccgtta ttgttccctc ggctcaagaa tttatacaac
     5761 cgccgcgccg agcgtctgcg cgagctggca gaaaataatc cgctgggtga ttacctgcgc
     5821 tttgctgcgc ttatcgccca cgcccaggaa gtggtgctgt acgaccatcc gctggagatg
     5881 gatctgaccg cgcgcattaa agaagccagc gcacagggca agcccccgct ggatattcac
     5941 gttctgccgc gtgataagca ctggcaaaag ctgctgatgg cgctgattgc tgagctgaaa
     6001 cctgaaatga gcggcccggc gctggcagtg attgagaatc tggagaaggc atcgactcag
     6061 gagctggaag atatggccag cgcactgttt gcctctgatt tctcatccgt cagcagcgat
     6121 aaagcgccgt ttatctgggc tgcactgtcg ctctactggg cgcagatggc caatctgatc
     6181 cccggcaaag cccgcgctga atacggcgag caacgtcaat attgcccggt ttgtggttct
     6241 atgccggtgt ccagcatggt gcaaattggc accactcagg gtctgcgtta cctgcactgc
     6301 aacctgtgtg aaaccgaatg gcacgtagtg cgcgtaaaat gcagcaactg cgagcagagc
     6361 ggcaaactgc attactggtc gctggatgac gaacaggccg cgattaaagc cgaaagctgc
     6421 gatgactgtg gcacttacct gaagattctt tatcaggaaa aagaaccgaa agttgaagcc
     6481 gtggcagatg acctcgcttc tctggtactg gacgcgcgaa tggagcaaga aggctatgcc
     6541 cgcagttcca tcaacccgtt cctgtttccg ggtgaagggg agtaatctct gacgttttcc
     6601 gggtggcgct ggtcgcccgg attacagctc atagggtgtg acgacaccat ctctcgtatt
     6661 ccgcgttacc cattcaagat catgccgcaa ttgtctgttt gcctcgttct tccctccaac
     6721 acatcgataa ggttatgggt atgtcactga tgacaagcgt atgattgcct gatgcgctgc
     6781 gcttatcagc cctaccaata aacttcaata tattgaattt gcatgacttt gtaggccgga
     6841 taaggcgttc acaccgcatc cggcatgaac aacgcgcacg ttgtcagcaa tctgattgac
     6901 atcatttttt cccattacca ttcctcgcgc agcttacgct gatattcgac accatcttcc
     6961 tcacaccctt gccagatgcc aggaacactg gttcttgctg tttgcgattg atttatcagg
     7021 tattgatcta ccgcttccct taataggtcc gcacgaggaa gattacgctg cacctcgaga
     7081 tcatcaagtt gcttaatcac ctcattcgat aaatcgagta aaattctgcc catatctata
     7141 cctaccagag agttcataaa tatctcgcca atatcatttt tgaatctatg gagagaaaag
     7201 tacccttgtc gaatctttaa agaaagctca tttacgaatc attttttatt tttgcgaggc
     7261 gttttccaga tatgatgtaa cagttgcagt tattataagt tcaagtaaaa cagccttgta
     7321 ttaataccag aataagggca tctgtttttt tatattcaag aatgaaaaat ttttttcatt
     7381 ccttatgctc cttacactga tcatcaacac caactacctc ccccttttgc ctgcctccct
     7441 ttgctttacg caccggaaat tgctgccgct ccagatattg ctcaaccgct tcgcgtaaaa
     7501 ttgcagatcg tgaaacctta cgccagcccc gaagtttgtt cagccgttta attgcctctt
     7561 ctgataaatg aagaaaaacc gtattcatcg ccattaccac caccttacct atattactca
     7621 ttaatgtatg tgcgaaaatt acgccggaaa atcatttaaa aaaagcggat ttttgcatgt
     7681 tgttttgttt ttgcgaggcg ttttccagaa ataataagag tgttgcagtt gtttcattta
     7741 ttactcaaaa atggtttagt tatcgcccag gatgaattat gggtgctatg aatatattat
     7801 attcagagat atttatttgc ataatctgaa tggtgtaccc gaaaaatgtc agcatcctaa
     7861 cagcacccgg atttaaggtg aaattaatca tccatcacag accggtcagt actgccacca
     7921 accggtcttt acttgttcat cgttataatt ttgttggtaa cagttcggga aacaaggaat
     7981 tccttccgga accccccggt atcatccgat agcattgacg tcaatcatca gcgcgacgat
     8041 gagttttttt aatcgcatca tagggtggcg acgttcagca cgattacggg tcatagcaaa
     8101 actcctgggc aggcgaccac tatgccgctt tcacaggagt tctgaatgct agtgctttct
     8161 gtatggcata accatgcctc taacggttaa gtgcttgcag cacatgttgc ggatcattgg
     8221 caatcgctcg cagcaaagcc tttgcaggcc cggtcggtga acgtcgcccc tgctcccagt
     8281 tacgcaacgt gtctacgctt acagaaataa gatcggcaaa tttagattgt gacaaccctg
     8341 aagcgcggcg tatttctttg attttcatag catcaacttc aaaagtccgc gaaggctgtc
     8401 gcgttccttc aataatttca ttcatctggg ccatgctgtg agtcaaccgc tcaaaaagtg
     8461 ccttatccat ttaccacctc gtatttaaca accggagcat tgctttttcc tggggagaca
     8521 gatcatcttt aataccctta cgataaataa gcagtaatct gatctcataa tgtttaacct
     8581 gatgaaagta aataaccctg acaccagcac gtttcccctt tcctcctgaa acccatcgaa
     8641 cctttcttaa cccaccagtt tcaggtatta cctcaccata gtcaggattc aaggccagga
     8701 aaaactgaaa acgactgaat tcatcgtcgg tcagcagttt ttgaacatct tcagtaaaaa
     8761 tttccgtttc gatgaagagc ataattatac gccattggct tagagtaaca atcctttttg
     8821 tacgtcattg acgtaccctt gaaaccacta gcttactgat agtgctccag caaaaccacg
     8881 tctctgccgg gtttgtcttt agatttgcga ggcgctttcc agatttttca cgcccgtgaa
     8941 tttccctgta tccatccagc aaaaacgggt gtataagact gtacattcat acagcaaaat
     9001 aagttctact atggcactgg aaaaaggaat tgcagagtta gtcgaaaagt ttatcgctgc
     9061 gggtcgcccc tcatcgcgtc aacaaagtat tgcgcagcgc agggagggct atatcgccag
     9121 cgctgtcctt gcgggtgaga cagaaacgcg ggtagatatt cagactatcg aacttgaagg
     9181 gatgacctta cgaatagtgt cgccgctgaa tgccccgaca ttactcccga cgataattta
     9241 ttactacggt ggctgttttg ttagcggcgg gttcgcaact cacgacaacc agctgcgcca
     9301 gttagcctat tacgggcaat gtcgggttat tgccgtccag tatcggctgg caccggaaca
     9361 tactttcccc gccgcccatg atgatgcgca acgaggtgca gaaatagtct ggcaacacgc
     9421 cgaacgactc ggcgttgata aacagcggat aactcttgcc ggtgacagtg cgggcggaca
     9481 tctggcgctg gtaactgctt tgcgattaaa gcgtgctggc gagtggcaac cggcacaact
     9541 catcttaatt tatcctatgc ttgatgcgac tgcgcatttc gaaagctaca tacgcaatgg
     9601 tcacgattac atcatcacac gcgacacttt actgagcggc tttgagatgt atctgccagg
     9661 cattgaacgc aggcatcctg aagccagccc tctttggcgc gatgatttca atggcttgcc
     9721 acctgtacat atcattaccg ctgaatacga cccactttgc gatgaaggtg aagcgttgta
     9781 tcaccgcatg acagggcaag gagtgcaatg cacctgccaa cgctaccttg gcgttatcca
     9841 cggatttttt cagttaggtg gtatcagcga ggccgcgcga tcggcactac gggatgttgc
     9901 ctggagagcg ggaaggtaac ttcggacctg ggccctgaaa aggttttcag attgaaaacc
     9961 ttttcagtca agtaaaacgc ccctactctt cttcgttccc gccctcttca tacgggccaa
    10021 atggcttcgc gggcagaacg atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc
    10081 caaagatttc aacttgcatc tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat
    10141 cgccgcttaa ggcaccaagg tcggctaccg catgaggttt accgctaatc ggcttgctgt
    10201 agcggatatg cgcatccgcc agaataatcg ttccgccgag atggcgttcg cgcagcatca
    10261 gccagataag cccccaaccg gtgagcgtcg ccagcgagaa taaactcccg gcaaacagcg
    10321 tatggtgcgg attctgattg ccggtttccg gcatggtggt gataaatttt tgcccggtat
    10381 attgttgaat acgcacgccc attttttcac taagcgggat atgttcgtac cacgcctgtt
    10441 gcagctgcgc gcaccagtcg ccgcgatgca aaatatcatc cagagtggcg acgggcttaa
    10501 tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt gatttctccc tgattaacaa
    10561 accccagctt ggcgaaaaac tccaccgcgt cttcacgggc gctacaggtc acgcgcttaa
    10621 cgccttcctg acgcgccacc gactccaggg tcatcgccat cagcgttcct aaccctttgt
    10681 cctgcacgtc cgggtgaacg gccataaagc gaatggacgc ttcattgtcg gcattaatat
    10741 acagtcggcc taccgccacc agattaccct gctcgtcgac gaccatctgg tgatgcgcca
    10801 tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg caggggctta cgcaacattt
    10861 cccagcgaaa ctgatagtaa cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat
    10921 acatagcgat actctctctt gtttcccgtg acaaccctgg aagctggctc atacctgcaa
    10981 ccagaatgtc acggggccat cattgaccag cgatacctgc atatcggcag cgaagcgtcc
    11041 tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg aaatagtcat ataacgcctc
    11101 tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc atccctcgtt cggtatctgc
    11161 ggcgagggta aactgggaaa ccaccagtac actgccgccc gcctgttgca cgttgagatt
    11221 catcttgcct tcggcatcgc taaagatgcg gtagccgagc acacgctcgc acagacggtt
    11281 tgctttctgt tcgtcgtcat ccttttcgac acccaataac accaaaagtc ccgcgccaat
    11341 ttcgcccgtc acttctccct ccacggtgac gctggcacgg gttacgcgtt gaattaatgc
    11401 aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt ttagtttgcg gtattccccg
    11461 agagtgacag taatttccgc gccaagcaag acgatacacc atgtccagta gacccaaaca
    11521 aagagaatgg ggatcaccgc cagcacaccg taaatgagct gatatgacgg gaacatggtg
    11581 atataaagcg cgaaaccttt ctttcctgct tcgaacagga gcgcggcgac aaacgcgccg
    11641 acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa tgctgtacag caaccagaag
    11701 gagatccacg acaacagcag cggaaaaata cgcagcacgt tgtcgatgac agtattgaga
    11761 tcgctcgccc agcgcaggga gagcaaatag gaactgatcg ccagactggc ccctgccagc
    11821 agcggcccca gcgttaaaat catccagtac acggcgaacg agtaaatttt tggtcgcgct
    11881 cgtttactgc gccagatggt attcaacgcg ctatcgatgg agtacatcag caataacgcg
    11941 gtgacgatca gcccgcacgc cccaacggcg gtcatcttgt tggaattggc aacaaattgt
    12001 tcgatatacc gctgaataac atcgccagta gcaggcagaa agttggcaaa aataaagtga
    12061 cgtaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg
    12121 gcaaccagcg gcactaatga gagcaacgac acataggcaa ggttacctgc cagggttgtc
    12181 atgttgtcct catcaatgcg ttgccagagt agttttagcc aggcccatag tggacgggta
    12241 cggtgcctgg ctttgtcctg aatggttttt agcataacac cttcgcgaaa tagtccggga
    12301 tggtggtttt atctttcacc agaatactgg taatgcccag ctgattggct ccttctatat
    12361 tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga aaaaccttcc gcctgcaaaa
    12421 catgctggta aattcgtgct tcaggtttgc gcatccccag atcttgcgac agatagatat
    12481 ggtcagcagc atcacgaatt tccgggtatt cttccggcca gaaggtggta tgcaggcggt
    12541 tggtattgga aagcaccacc acgcgatgcc cctgctcacg cagtttatgc atgatggcga
    12601 tcacttccgg gcgcagcgca acaaacaccg cctgccagcc gtgagagaac tgctcgtagc
    12661 ttagcggtag agccatctca tgacacagcg cctctgcgaa cgcttcgtcg ctaatttccc
    12721 cacgctcatg ctgatgaaac gcctccccca tatgaaaact cttcttaagc gatgccagcg
    12781 gaatacgcgt taaatcgctc caggctccca gcacacggtt aaagtcgata tcgacaatca
    12841 cattacctaa atcaaagata tagagcattt ttgcctcctt tcgcgccaca agaaattaac
    12901 tgtagcggga aaggatgggt ttggctatgt ggcaataagg gtaatcttgt gatgctgatt
    12961 gcgaaaaagc agagtaattt cactggcttt ttcgcaatag atgaatggtt aagattcagg
    13021 tgtaaattgc caacgagttt cgatgaccag cggcctatct ggtgtcacca attccggcgt
    13081 tagatttatt gcgttaggtg cctgagtcag cgggttaata caggtggcat cgggctgttt
    13141 atcaaatacc accagactgt ttgcagaaga gatcatggtc atcgcgagtt taccaggcca
    13201 cagtaactta accttcaccc cgtcataaaa accaaaacag tcatcccagg gaccggcttg
    13261 aggagagata cgatttccag ttggcagttc atcactaccc gcttcttctt gccagtccgc
    13321 atcaaaaagc acctgcaacg attctgtatt ttgcggagtc agctttttcg caaaccacgg
    13381 atgccagcct gcggaggccg ggaatgtatc ggcatatgaa tggacttcca gttgcaaaac
    13441 cagcgcatcg ttctccagca aaaaagtctg aatcacttcc ccttgccagg gccacggtga
    13501 agccaggggc atacgtaaag tcagggaatc tgctgttttg tcgatgattt cccacgttga
    13561 gtaacaagcc ataccatgca acgcatgtgg tggtttattg gcgggtaaag aataacattg
    13621 cccaccagca ttcagcgtcg cattccccag tcgccctgcc cacggcacca ttgggaaaca
    13681 gccatactga aaagctctgc gctgcggctg ccattggcgt aatacttcag aaccaaatgc
    13741 ctttaacgag gttatcctgg caccatcatg cggataaatc tgcaggtgaa tattgccttt
    13801 ttccagcgtc actaactctc ctacaggccc aggccatggc gcgtcaaacg cctgttgtag
    13861 cgccaggtaa tgctcgtttt ttcgttcagt tttcatgaga ttgttccctg atgtagcggt
    13921 aagaagcggt ttcttgttca tcggtcggct caatcagcca gacgcaaaca aaggaaagaa
    13981 ctgagacagc aaataaaaac agagcgacat accacggttt tcctccccct agcgccagca
    14041 aacttgaggc gataaggggg cttagtccgc caagaatcgc ggaaaattga taagcaaaag
    14101 aaaggccggt atatcgtact ttggtaccga acatgcgggt gaaaagtgtt ggctgcacgg
    14161 caaacatcat cgtcggacct aagttgtagc cgaggatcat actgcaccag atgatgagcg
    14221 tacttttgct atccagtaat aagaagaaag gaaacgcgaa caggacacaa aatcctgctc
    14281 ccgacaaata cagacttttt tgacctatac gatctgataa ccatccaacc aatggacaac
    14341 tgaaaatacc gatcgccgag gcaatcaaca tacccgtcag gggaatatct cgcgacaatg
    14401 ctaattggct ggaaatatag acaataccaa aggcgttaat aatattagag gagacacttt
    14461 ccgccagcct cgcacccagt gccaataaaa tatttcgtgg atgttttttg aataattcaa
    14521 tcaacggagg aacggacttt tctttattat tcgtcgtttt ttgcttttga aaatccagcg
    14581 tttcttcggt atgcaggcgg ataaacagcc caacgatcaa catcaccgca gaaagccaaa
    14641 acggaatgcg ccatccccag gagagaaact gctccgaagt aagaaaatgg ttacagagag
    14701 caaaaatacc ggttgccagc ataataccca cagacgcagc cgtttgcggg agtgaaccta
    14761 aaaatccgcg tcgggattcg ggcgctgatt ctatggtcat caatgccgct ccgccatact
    14821 cgccaccaag accaaagccc tgaattaaac gcagcgccat taatatgagc ggtgcccaga
    14881 tcccaatttc ctgatacgtc ggaataaaac caatcaaaaa tgtggagcaa ccgacaatag
    14941 ccagtgtcca gataagtgtt atttttcgcc caattttatc gccaaaatgg ccaaagacga
    15001 taccacctaa aggacgggtc aaaaaaccaa cgccaaaggt agcaaaggcg gctataagac
    15061 ctataaaagg atcgctgtct ggataaaaca gcggtgcaaa taccagtcct gctgccgtgc
    15121 caaaaataaa gaagtcatac cattccatta acgcgcctgc gaaactgccc ataacgacac
    15181 gtctcatggc tttaccggaa agcccttcac gcggtgcggg taacttttca gacataattt
    15241 tcctctcctg gtaggcgaga aatctgggat acgaaccact aaacgtgggc agtttgaatt
    15301 aatgcgcatc ggtttaagcc gatgcgcatt aatttatttc ttcaattcag ccagaacctc
    15361 tgcatcctca gcatcaacat ttgcacccag tggttcccaa ttaatcaata cgcaggtcag
    15421 gaaccccaag aatggcacga ttgccaacca gtaaaacgct tcagttttca acgcccccca
    15481 cagcatcggc cagaaaatga gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa
    15541 tccgacccca gtagggcgaa tagcgggagg ataagagagt gtggcaatgg tcatgcccag
    15601 cccaccagga cccgctgaat ggaagaaaat aattgatccc aataacccta aagacagcca
    15661 tggattagtc gttgccagtg cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc
    15721 atacatcgac tgtcggcgag tacccagtcg ttggagaata aacgagccga tcatcccgcc
    15781 tgtcacccca caaagggcat tcacgacggc agaacccgtc aacgcaccag taagcccgcc
    15841 acttattatt cccgccaacg taagaggaag ataaacacct acggcgttat attgccacgc
    15901 ctgcatggtt gccacgacac agccaagaat ggtgcgtttg cggtaacgat cattaaataa
    15961 gatgccatat ccaccactgt atttattctc tgctttttcc gagaatttcg cttctgttgt
    16021 actcgatgcc gcaacgcgag cctgaacacc ataacgtttt cccagaatgt cgcacgcttc
    16081 ctggtaacgc ccgacgcgtg cggcccacat tgcggattca ccaataaaga agtaacgcaa
    16141 aatcatgaag atgactgcaa aaatagctcc gatgaatatt ccgtaacgcc acaattgttc
    16201 ttcagcgata cccgacagaa gcagcggcaa gagcaccaaa taaacaacag ttgtcgaaac
    16261 ataccaggcc atttgccaga ggttgaccga cgttccggtt ttcttcgacg ttgaaccacg
    16321 aagctctgcc acggcattgg ttgccaaagg gaagtcgatg cctaaaccaa agcccatgat
    16381 gacgcggcac accagcaccc accagatact gggcgcaata gctaccgcag cagccccgat
    16441 ggtgcacagc cccatgccaa taataaatgc tcgcttttgc ccaaagcggt ttattactcg
    16501 gttcgctaat aaaccgccaa ttaacgcggc gattaatact gacgcattaa cggttgcggc
    16561 aaggcccgga gaaatgccaa attgagcggc aatgtattta ttaccaaacc ccaccatcgc
    16621 cgcctggtag gcatcaatca atatgccgcc caaagcaata aaaataacgc cgatactggt
    16681 gttgattgca gagttcttat taacaatatc aattacgtcc tgtggccgac taattgaata
    16741 agttgcagga ctggtgattt gactcatcgc agtgtccttt ttaattgttg tagggtggcg
    16801 ctgacgtgac gcagcgccag acagagattt agaaaatcat tccggcgcaa attgcagcat
    16861 gacctttatg gagtcatttt gctgcgcgcg ttcgaatgcc gccgctgcat cctcaatggc
    16921 aaataaatcg gtaaccagtt cactggtatc aaaccgccca tccgccagcc attcgataac
    16981 agcatcgaaa tcttcaggga tgtacatgcc agagttgagc aagtcacgct cgaaacgttg
    17041 catcatcggt aatggtattt cacgcggccc acttggtacg cccatgcaca cgacggtgcc
    17101 tccggcatat acacgggtac atgcatcgtt aagcgtggcc tgcgctgcaa cgacatcaat
    17161 cgcccccgta aatcgcacat ctgcggccag ctcacctggt gcccaaactt ctgctgcgcc
    17221 cagctttaac gccagcgcgc gtttggcagc gtcaggttca atcacggtaa tcttacctgc
    17281 ccccataatg cgcagtgcct gcacgatgga taagcctatc gttccggcac caatcaccag
    17341 tacgctttcc agagaagctt tcggcattcg gttaacgcag tgacgggcac aagccgccgg
    17401 ttcggcaaag gccaacactt ttagcggtaa agaggctggt gcgacatggc aattacgcgc
    17461 aggaacaatg tgttgtgagc gagcaaagcc cggtgcgcga aaaccagcaa cctgcggtgg
    17521 ttcacaaaga ttaaaacgtc ctgctttgca ggctcggcat tccatgcaag ccatgatggg
    17581 atcgaccaca acatgatcgc ccggctgtac atttttaacg tcgctgccaa cttccgtaac
    17641 gcgcgctgca atttcatgac cggggacgac aggtggttta gcaaacggat gaccgccttt
    17701 cagaacatgc agatccgaac cgcatatccc atagaacaca ggcgcaatcc gcacttcgtg
    17761 cggtttaagt tcctggtgtg gtacttcaac atattgagtg gtgactttcc caatatctga
    17821 aaataaaacc tgggtaattt tatccattgt tattctccag aatcgatatc cataggggat
    17881 tagcgttgcg cgataccttc atcgacaatt tctgccaacc gttgtaaacg aggaacagag
    17941 acatcgcgca gcatagtttg tggatcttgt gcaccaacaa ccgacgccca aaccgctcgt
    18001 ccagccagga agcctgacgc accacctttc atggcaatac ttactgcacg gccaaaaata
    18061 tcggcatcaa cgccagaaga aagaattacc cacggcatct tcatctgatc gttaagttgc
    18121 tgacaggcgg caagtaaggt tttttcatcg cctttgcccc ctaatggcat ctcggctttg
    18181 taaagatcag cctctgttcc accaagctct gcggcggcgg caacaatggc gctttcacga
    18241 tcaaagtccc aaccgcgacg aggtggacgc accacaggtt caataatgct aaccagcccg
    18301 gcactacggc agcgacgaac aaatttatca accattgcca gacgttcctc tgcgggttca
    18361 tcttcacgcc agagtacaag taatttcatt gccttagccc ccatttcacg ggctttatgc
    18421 gggtcgacgg acatatctat ttcaacgcta tcaaccggga tgccattacc tgggataaaa
    18481 agatcggcag cgacaatcag gccgcaagaa ttagcaacag cgccctgctc gactatttgc
    18541 tcaagacaaa attgtttatc cgccagaacg gcagaagcat aaggagaaag gattcgggtc
    18601 gcggcaactt taaaatcggt gagtacactg tcggcgatgg gttttggctg ccctgctgcc
    18661 gcaaacatca agcgcatggc ctcacgctga tcgacagcta acatggcaaa tccaccacct
    18721 gggcgggaga tatctttcag cgtaaatggg gtgaagtggg atgacataga aactcctgtt
    18781 aatagataaa attaaagacc agcctgctgg cgaacctgat ttagcagggc actccagtcc
    18841 tgccgccctt tgccgtcttg acttgctgca gcgtaataac tttctgctgc aatccccata
    18901 ttgagcggtg cccccgtctc ccgagcgacg tcaactgcaa tacgcagatc tttcaacgcc
    18961 agatccacca tgaaggcggg tgaaagatcg cctttcagca ctttgcaagg ccaggtggtc
    19021 gtaaaatgtc ctttccctgc tgccgtacca ctcatcacct taattgcgac atcaagattt
    19081 aatcccagtg actcacataa cacagcggcc tcggaggaca gcgcattaag cgcaatgctc
    19141 atatagttgt taattagctt cacgcggatc cccattcctg ggccgccagc ctcgaccagc
    19201 tcattaccca tgcacatgag aatcgggcgt gcacgtgcaa tctgctcatc agtgccaccc
    19261 gctaaaatca gcaatgtgcc atcaatcgca ttcacggacg ttctgcccac gggggcgtcc
    19321 atcatactga tgcctttttc ttgtaactcc cgtattaatg catcggtctg taagggatga
    19381 atagtcgaca tatcaatcaa taacgcgtca gatgaaacag cctcacagac gcctttttcc
    19441 cccaacagaa cctgtcgaac aatgtcacca ttgggaagca tagtgatgat aaattcagcg
    19501 cctgctgccg cttccgctgg ggtttgtgcc gcagtggccc cctgcgtaac tagcgcatcg
    19561 acggcctgtg cattgacatc aaatacctgc aatgaatgcc ctttttgcaa tagattgtta
    19621 gccattgggc tacccatctg ccccagcccg agaaatgcaa tggttgccat gaatactctc
    19681 ctgtctgaca ttgacaaaat aatgacaatt gaaaattcgt tttcgtctgt ttttgagcat
    19741 atttgtaatt tatgttcaaa aacacgacac gcatcactaa tttgatcatt tgtgtcattt
    19801 aaattgaccg aagacgacaa aattaaacag gacaagacaa atatgaccaa aattgcatgc
    19861 gtgggtatca cagttcagga caggatttat tctttgctca ctctgccgga tggcggcgga
    19921 aaatatcagg caaatcatta tcttgagatt ggtggcggcc cggcagcaac agccgctgtt
    19981 gcaatcgcaa agcttggcgt agaggttgat ttcatcggtc gcgtcggcga tgacagttgt
    20041 ggaaatacct tacttgcaga acttgagggt tggggcgtca ataccgcatt ttgccgccga
    20101 tatcctaacg cccgttcatc gcaatcagcg atcctcgttg atcagcatgg cgaacggatt
    20161 atcgttaatt atccaagtcc cgatctcggt actgatgccg aatggttaga agcgatcgat
    20221 ttctctcgtt atgatctgat tctggccgat gttcgctggc atagcggtac tgaaaaagca
    20281 ttctcgctag cgcgtcttgc gggtgtgaca acgcttttag acgccgatat gacaccgcag
    20341 gatatttctc cacttgtcgc gcttgccgat cacgccgtgt tctcaacacc agggctgaaa
    20401 cgcatgactg gcctgcagtc acctgaagaa ggtttgttcc aggcaacgac acaaacagca
    20461 ggtaaagtgt atgttacgct gggttctgaa ggatcgttgt ggattgaaga tggtcatttg
    20521 tgtcaacagg aggcattttc tgtcaatgtg gttgatacca ctggagcagg cgatgttttt
    20581 cacggcgcac tcgcggtagc gttggcagaa aaaatgccaa caaaagaggc tattcgtttt
    20641 gccagtgcgg ttgccgcgat gaaatgtacc cagcctggcg gtcgggccgg cattcctaat
    20701 cgtgagcaaa ccgaatcatt tttgtcactt tatgcgtaaa atggtaaaca tctaccgagg
    20761 aggggcaagg gagtattcat gagtatcatc gaagtgacag gtaatccacg ccatgaccaa
    20821 ctcgttcatc taatcgcaga acgcggttat atgaatattg aagaactggc acaactgctg
    20881 gatgtttcaa cgcaaaccgt gcgccgggat atccgaaaac tcagtgagca gggcttgatc
    20941 acccgtcatc atggaggggc cgggcgcgtc tccagcgtca tgaataccgc ttttgagcaa
    21001 cgggaacttt cgctcaccgc cgagaaacgg gcgatcgctg aggcagtcgc cgattacctt
    21061 cccgaacgct gtaccgtctt tatcaccatc ggtacaaccg tagaagccgt tgccagggca
    21121 ttactcaacc ggcgtgattt acgcattatc actaacagcc tacgtgtggc acagattctt
    21181 tataagaatc aggatattga agtgatggtg ccgggaggca ctttacgcgc tcataacggc
    21241 gggattatcg gcccaggagc cgtggatttt attgaaggct tccgcgcaga ttatttaatc
    21301 accagtatag gtgccattga acatgacggc accctactgg aatttgatgt aaatgaagcg
    21361 ttagtcgcga gaacgatgat taaacatgcg cggaatacat tgttagtggc cgatcatacg
    21421 aagtttgccg cgtctgctgc cgtttcaatt ggcaatgcac ggaatgtcag ggcttttttt
    21481 acagatgccc cgcctcccaa ttctttctgc cagttgttaa gtgaagagaa tgttgaactg
    21541 gtggttgccg agcaggaagt ttcctgaaat tgaaacttcg aaaaatccgc tggggattca
    21601 gcggatttta ttttgccgga tgcggcctac ggttgatacg gttttcgttg gccggattaa
    21661 gcacatccgg ccattaatta agcttcagcc gccagcatct ctttcttacg ttggctaata
    21721 cgcttaacca gcattacgga aaccaccgca ccacagaatg cgaagcaggc cataatgcca
    21781 aacacgattt tgtagccaat aatccccgga tttaaatcca gaatgtagcc atacagactg
    21841 aagcagaaca tcgccggagc gtaaccaata aagctaccca acgccatcgc cgcgcctgtt
    21901 ttattttcag caatttttgc ttcgccgata ggtgcaaaaa atacggctcg ctgtgtaaag
    21961 actatcgcgc caaagcccag cgtacatgcc atccctaaat agaccggcat actttcgtgc
    22021 ggcagcataa tcaacagtac gagcgcagcg gtactgatga taaaggtgta gcatagatat
    22081 ttactcggcg atttcaggat cttatctgaa atcatgccac caatcggtcc accaatcatt
    22141 ttcagacagt attggttaat gatgccgtaa gcccccacca gcgcaacggg caatagatag
    22201 atgtttttca ggaatggaat gaagaatgtc aggccacagt aaaccgcgta aacgaagaag
    22261 acgttaaaag cgataagcca gatggttttg tctctcagca ccgaggtcat actggtgttt
    22321 tgcgatgctc cgtcttcttt tttcacctca acggacggtg cctcttcttt gtcattcagg
    22381 ccaaagaaaa taataatgcc tacggcaatc acaataaggg aatagaacca gatgcctgct
    22441 ttaaaaccca ataagccact gccaaaccag gtaaatactg ccaacgcaga aaatgccacc
    22501 acggtatcga caatgccacg ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg
    22561 ctattgccca atcgacttac cgacttgagc aagaccggcc agttcatcat gtcgcaagta
    22621 acgccaaata gcgcccagac aaagaggatg ccccaatacc ccggcattgt cgtcaaataa
    22681 acacccagta atcctgtcgc aatgagtgag aatgacatgg tgtatctgcg cggtagttta
    22741 tcggcaaaat aaatagacag aaaaaaaccc actgtcgtga caaatgagtt taccgacata
    22801 gcattaccaa tttgaccatt ggtcaaatgg aaatattcct gcatcgggat ataaaacgca
    22861 tctttcagcg acggtaattt ataaattgta ccgccacaca atgttaatag actaaataac
    22921 gcccatttct ttttcgtgag cataacatcc cctaagcgat ttttaaaatc actgttttaa
    22981 aggcaataag ataccgacca ttcatatgga atggttttat taactgcgta tgagttaaaa
    23041 cttattagtg gttcagaaga tggtttgcct cttgtttgat ttgttgtagt acgttacgca
    23101 catgatttat ctgattaatt gcatcttctt tttctttacg taaccgcgca tccagtaaat
    23161 gattttctgg caatggtgtt tcactcatct ggcgataagg gatccacgga ttatcatctt
    23221 cgtcttcaaa gcggaacgcc ggcgcatagt aatcaacctc ttcttccagg ccatatgccg
    23281 tcacctgcgg ctcatcatca cccaggcata taaggtgcgt taataacgct ttgaacggca
    23341 tatcaccctg acctgaaata caggctttat gacccaggcc acccggttct ttaacgatca
    23401 aggcatcttt gatatggacc tgggtaatat gcggcgccat cgtttttaaa gcgtcgatgg
    23461 gatgctcatt tgcattgatc atgttcgcaa aatcaaacag taaggaaaga gattccatct
    23521 cactttcttt gaccagcgac accagctcat ggctttttaa atcttcatgc tgttcgatcg
    23581 taaaagtcag gccgctgtcc tgatacgttt cccgtacata ggcaatgtcg ttagcgatga
    23641 tcgataatac gtcgcgcaaa ttaccttcat aacgtggata aaaacgtacg gacgatgccc
    23701 cagttttcaa cgcaatggcg acggcttcgt caatagatgc cttatctgag gcgctggttt
    23761 caatatgaat atcaaggttc agacggcggg ctttatctcc aaaggcagag agttctttat
    23821 cgtccatatt accaagagaa aaacgctcgc catccagaac atggattttg acgccacgta
    23881 gattattttc acttgcgata tcaagtaaat cagcaggcaa cactctttcc agccgcatat
    23941 ttaaatgaaa ggcataagca tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct
    24001 ttcttgcgtt atttattgtc accataaaac acctacagat taatgatgag gtctacggcc
    24061 tcaaatcgcc aatattcaat atctatctgg acaatattgc cttgctggtc ggcgcgatgc
    24121 ttttcaatta agatcgctgg catacccgca gaaccgccaa gatacttgct gatatcgccc
    24181 ggcattctga cgggtttgaa aatcaatttt tttactaccg tctctttttg gtagcacttt
    24241 tcccagactg cagaaaatga gtggttttca agttgttcaa taaaacccgg agcaacttca
    24301 ggattaatat atgtttcgtg atagaaaact ttatgtcctt ccagcgcccc ccagccggtg
    24361 atgcgataga gttcagttga tggtgtcact gcaatcagtg gcgcgagcgt ctcgggaata
    24421 tcgctggtac ggtttttctc ggtaagcccc caggaaggct ctcgtccttg ctcaattgcg
    24481 gcccgctgaa agctcgccga caactccgga ctgtaattaa atcgcggctg ggtcacgaac
    24541 caccccttac gatccttacg gaagattttg gattctgcct cgaggttcaa caacgcctgg
    24601 cgcagcgtca tacgtttaat gcccagcagt tctcccagct ctctttctga agggagtttt
    24661 ccacccggcg tggtgatgcc atctttcaac caccgatcca gtttctcttt tgcattttct
    24721 acggtggatt gattctccgc cattgtttaa tccttttggt ttaaaccaga tgaagcatct
    24781 tacgcttacg ccaaaaaaga aagcaattaa cggcgtatga ctttgcagat aattgtgaca
    24841 aagctctcta ttgacgtaat cccagactta taagggagat actcgcattg gtgtgcgctc
    24901 acgacaggta aaaaaaaacc tgccagcgat ggcaggtttt ttataactaa aaaacgaaat
    24961 taatcgtctt tcggtgcgcg gttggcgcgg cgacgatcgt tttccgtcag gtgacgttta
    25021 cgaatacgga tagaggtcgg agtcacttct accagttcgt catcatcgat gaactccaga
    25081 gcttgttcca gagtcatgcg gataggcgga accagaacaa cggcttcgtc agtaccggaa
    25141 gcacgcatgt tggtcagttt cttaccggtc aggcagttta ccgtcaggtc gttagagcgg
    25201 ctatgaatac cgataatctg accttcgtaa acttctgcac cgtgaccgag gaacagctta
    25261 ccgcgatcct gcagaccgaa cagcgcgaac gcgaccgctt taccctgacc gttagagatc
    25321 agtacgccgt tctgacgctg acccacttca cccggacgta cgtcgtcgta gtggctgaag
    25381 gtggagtaca gcagaccagt accggaagtc atggtcatga actcagaacg gaagccaatc
    25441 agaccacggc ttgggatcac gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg
    25501 tttttcaggt cgcctttacg ttcgcccagc gcctgcatta cagaaccctg atgctgttct
    25561 tcaacgtcca gagtcacgtt ttcatacggc tcttgtttac gaccgtcgat ttcacggaag
    25621 ataactttcg gacgggatac cgccagttcg aaaccttcac gacgcatgtt ttcgatcaga
    25681 acagacaggt gcagttcgcc acgaccagaa acgcggaacg catcggcgtc ttcggtttct
    25741 tctacgcgca gcgcaacgtt gtgtaccagt tctttgttca gacgatccaa gatctgacga
    25801 gacgttacga acttaccttc tttaccgcag aacggcgagg tgttaacgca gaagaacata
    25861 gaaacggtcg gctcatcaac ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa
    25921 acggtgtcag aaatgttcag ttcgccaagg cccgtgatcg caacgatatc gccagcttcc
    25981 gccagatcgg tttcgatacg ttccagaccg aggtggccca gcactttacc gactttcgcg
    26041 ttgcgggttt tgccttcgct atcgatgata gtgacctgct ggttcggctt cactttaccg
    26101 cgcttgatgc ggccaatgcc gataacgcca acatagctgt tgtaatcgag ctgagaaatc
    26161 tgcatctgga acggaccgtc aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc
    26221 tggtacagcg gggtcatgtc ttccgccata tcttcgtggt ccagacccgc gataccgttc
    26281 agcgcagaag cgtaaacgat cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg
    26341 aacagatcga atacctgatc cacaacccaa tcaggacgcg cgccagggcg gtcaactttg
    26401 ttgataacaa caatcggctt caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt
    26461 tgcggcatcg ggccgtcaaa tgcgtcaacc accagcagca ctgagtctac catggacatt
    26521 acacgttcaa cttcaccacc gaagtcggcg tgccccgggg tatcaacgat gttgatacgg
    26581 taatcattcc atttgatagc ggtgtttttc gcgaggatgg taatcccacg ctctttctcc
    26641 aaatcgttgg agtccatcac gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat
    26701 tgttggagca gcttgtctac cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg
    26761 atattacgca atttttcgat cacaactttg cctcaggcat tagaaatagc gcgttattgt
    26821 acacggatta atcgcactac aaaacaggat cacaaacatc ctccgcaaac aagtattgca
    26881 gagtcccttt gtgatcgctt tcacggagca taaaaagggt tatccaaagg tcattgcacc
    26941 aacatggtgc ttaatgtttc cattgaagca ctatattggt gcaacattca catcgtggtg
    27001 cagccctttt gcacgatggt gcgcatgata acgcctttta ggggcaattt aaaagttggc
    27061 acagatttcg ctttatcttt tttacggcga cacggccaaa ataattgcag atttcgttac
    27121 cacgacgacc atgaccaatc caggagagtt aaagtatgtc cgctgaacac gtactgacga
    27181 tgctgaacga gcacgaagtg aagtttgttg atttgcgctt caccgatact aaaggtaaag
    27241 aacagcacgt cactatccct gctcatcagg tgaatgctga attcttcgaa gaaggcaaaa
    27301 tgtttgacgg ctcctcgatt ggcggctgga aaggcattaa cgagtccgac atggtgctga
    27361 tgccagacgc atccaccgca gtgattgacc cgttcttcgc cgactccacc ctgattatcc
    27421 gttgcgacat ccttgaacct ggcaccctgc aaggctatga ccgtgacccg cgctctattg
    27481 cgaagcgcgc cgaagattac ctgcgttcca ctggcattgc cgacaccgta ctgttcgggc
    27541 cagaacctga attcttcctg ttcgatgaca tccgtttcgg atcatctatc tctggttccc
    27601 acgttgctat cgacgatatc gaaggcgcat ggaactcctc cacccaatac gaaggtggta
    27661 acaaaggtca ccgtccggca gtgaaaggcg gttacttccc ggttccgccg gtagactctg
    27721 ctcaggatat tcgttctgaa atgtgtctgg tgatggaaca gatgggtctg gtggttgaag
    27781 cccatcacca cgaagtagcg actgctggtc agaacgaagt ggctacccgc ttcaatacca
    27841 tgaccaaaaa agctgacgaa attcagatct acaaatatgt tgtgcacaac gtagcgcacc
    27901 gcttcggtaa aaccgcgacc tttatgccaa aaccgatgtt cggtgataac ggctccggta
    27961 tgcactgcca catgtctctg tcgaaaaacg gcgttaacct gttcgcaggc gacaaatacg
    28021 caggtctgtc tgagcaggcg ctgtactaca ttggcggcgt aatcaaacac gctaaagcga
    28081 ttaacgccct ggcaaacccg accaccaact cttataagcg tctggtcccg ggctatgaag
    28141 caccggtaat gctggcttac tctgcgcgta accgttctgc gtctatccgt attccggtgg
    28201 tttcttctcc gaaagcacgt cgtatcgaag tacgtttccc ggatccggca gctaacccgt
    28261 acctgtgctt tgctgccctg ctgatggccg gtcttgatgg tatcaagaac aagatccatc
    28321 cgggcgaagc catggacaaa aacctgtatg acctgccgcc agaagaagcg aaagagatcc
    28381 cacaggttgc aggctctctg gaagaagcac tgaacgaact ggatctggac cgcgagttcc
    28441 tgaaagccgg tggcgtgttc actgacgaag caattgatgc gtacatcgct ctgcgtcgcg
    28501 aagaagatga ccgcgtgcgt atgactccgc atccggtaga gtttgagctg tactacagcg
    28561 tctaatagtt gaagttgtac tacccggcgc aacaacgccg ggatttagtt gccgtggaaa
    28621 ctttcagccc atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt
    28681 ttatccgtaa aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat
    28741 ggcaacaggc acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt
    28801 gttaatcgat gacaacctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc
    28861 ccaaagctcc cgcaaattgt ttggtacacc gttaccggaa ctgttgagct acttctcatt
    28921 aaatatcgag ctgatgcaag aaagtctgga ggcggggcaa ggttttaccg ataacgaagt
    28981 gacgctggtc atcgacgggc gctcgcatat cctttctgtg acggcccagc gtatgccgga
    29041 cggcatgatc ctgctggaga tggctccgat ggataaccag cgccgcttaa gtcaggaaca
    29101 gctacagcac gcccagcagg ttgctgcccg tgatttagtg cgcggcctgg cacatgagat
    29161 taaaaatccg cttggcggtt tacgtggcgc ggcgcagcta ctcagcaaag cgttacctga
    29221 cccatcacta ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaatct
    29281 ggtcgaccgt ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa
    29341 agtagctgaa cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat
    29401 tcgtgattac gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt
    29461 cttgctgaat attgtgcgca atgcgctaca ggcgctgggg ccggagggtg gtgaaatcat
    29521 tctgcgtacc cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc
    29581 gcggattgat gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt
    29641 ttacccgatg gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgctcgtaa
    29701 tttgattgat cagcattcag gcaaaattga atttaccagt tggccagggc ataccgagtt
    29761 ctcggtttac ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg
    29821 gtagtcgatg acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggggcaggt
    29881 ttaacctgta cgacgtttga gaacggcgca gaagtgctgg aggcgctggc gagcaaaacg
    29941 ccggatgtgc tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag
    30001 cagattaaac agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg
    30061 gatgctgccg tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat
    30121 atcgacgaag cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag
    30181 ccgcgtaatg ttcagcttaa cggcccaacg accgatatca tcggcgaagc gccagccatg
    30241 caggacgtgt tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac
    30301 ggcgaatccg gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc
    30361 gccaaagcgc cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca
    30421 gaactgtttg gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt
    30481 tttgaacagg ccgatggcgg tacattattc ctcgacgaaa ttggtgatat gccgctggat
    30541 gtgcagacgc gtttgctgcg cgtgctggca gacggtcagt tttaccgcgt tggcggctat
    30601 gcgccggtga aagtggatgt gcggattatc gctgccactc accagaatct cgaacagcga
    30661 gtgcaggaag gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat
    30721 ctgccgccgc tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag
    30781 gttgccgcgc gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct
    30841 ctgacgcgtc tggcgtggcc aggcaacgta cgccagctgg aaaacacctg ccgctggcta
    30901 acggtgatgg ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa
    30961 tcaacggttg cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg
    31021 cagtgggcag acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca
    31081 gagctggagc ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa
    31141 gcggcgcggc tactcggctg gggccgcaac accctgacgc gtaagttaaa agagctgggg
    31201 atggagtgat tcacagcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt
    31261 tacgctttac tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt
    31321 atgctggaat caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa
    31381 actgccgttg ctgccgtgct gtgtgccgca atgattgggc tgtttagttg agtttcagat
    31441 tgctgatgaa gtgctctttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
    31501 aaagcaatat tttcaacagg ttttgtaggc ctgataagcg tagcgcatca ggcaatcttg
    31561 ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc cattcactat
    31621 ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg tatcaaagca
    31681 catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc ccttctcatc
    31741 cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt ccgcaaagta
    31801 atcagcgaag tgcaaatccc actgtttctc aataggggcg taatccagac ggaagttgca
    31861 gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg caataccacg
    31921 ccacagcgca ttgccttgtt catccacttg ctgatagtac tgcttcaact ctttctggtt
    31981 ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca gcagatcggt
    32041 atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac gctgggccac
    32101 cgccagctcg tcatccggac gggcaaagtg atccataccg ataaactgat agcccgattg
    32161 cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac tcggcaggtc
    32221 agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt agttaaagac
    32281 actcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga aactctccgg
    32341 cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac caatctcacg
    32401 cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa ccagacgttg
    32461 cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt cggcgcgtaa
    32521 atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa tctccgcatc
    32581 ggcattgaac tggaagtttt cgcgcagcag cttcatcagg cggctgattt gcgctttatt
    32641 cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc cggcgaacag
    32701 cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg ccttgtgctg
    32761 ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc agaacgggat
    32821 atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta aaaacgcctg
    32881 ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc gtggcccgga
    32941 atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag acatgctcac
    33001 tccttccgat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt tcgggtcatc
    33061 agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac gaataaccac
    33121 accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac ggcggcaatc
    33181 cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc ttcctcttct
    33241 tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg atccagttta
    33301 gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag acgttccagc
    33361 agcgcatcca gacgctcatc cgtttccagt aattccaact ccgcctgcgg tgaaagcata
    33421 ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcacgcc caatggaata
    33481 ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct gcctgacgtg
    33541 gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt ctgacgcttg
    33601 cgatcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt tgcatgacct
    33661 ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc cgttttattc
    33721 agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc gacagattac
    33781 tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct tgcaggccgc
    33841 caacacaccc tgtttttcct ttagccggaa caatccgtgt tgattccatt tccgtttaat
    33901 tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg gcgctcctga
    33961 ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct actttacgcc
    34021 tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga gttttatata
    34081 aaagcaattc actgatttat atagcttatt tgacatgcat cgcagaattt tctctgctat
    34141 ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt acaacgctca
    34201 cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc tttgactgac
    34261 ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga tgagtgcgcc
    34321 tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag gccgttccaa
    34381 cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg ctcgtacctc
    34441 aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg gcaagcgtct
    34501 ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc gcaaatggca
    34561 gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg tggtgctaat
    34621 ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg cggtagacag
    34681 caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg gcgcacgtaa
    34741 agcgcaattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg tgcaggttga
    34801 aacgttttct tcgttgaaga aacaaggcgt ggacaagctg cggcagaaac tggatacctg
    34861 gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt cttgccttaa
    34921 tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa ttctaccgtt
    34981 ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca ataaaaaacg
    35041 ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt acgtgaagta
    35101 aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact ttactctttt
    35161 ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata ggaatttcta
    35221 atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg gcatgttcag
    35281 gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc agcaacggca
    35341 catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg gcatcaacat
    35401 catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata cgtacacgcg
    35461 gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata atgtcggcgg
    35521 cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga cgagcaccat
    35581 tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca acgtagccct
    35641 gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg tagcgttcga
    35701 agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc cgcgccagac
    35761 cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta cggcgttgct
    35821 cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc cggtggatat
    35881 cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg cgaaagatgc gccataatgc
    35941 gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc gcaataaacg
    36001 cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg ttaggatcgg
    36061 ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc acacgcccgg
    36121 ttttcgggtt gatcatcagc ggcagcttgt cggtgtaggt cgatttcagc ttcgccagac
    36181 cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct tccagtacct
    36241 cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata ccctgttttt
    36301 caaagagaat ggtttgtaac tgcttggtgg aagaaaggtt aaattcctca cctgcaattt
    36361 catgcgcttt cttttccagc tcagccagac gaagggtgag ctcttcagaa tgattgtgca
    36421 gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcact ggcaccagcg
    36481 gcatttcgat attctcgaag acgttcaacg gccctttgtg tttttgcaga tccggccaca
    36541 ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt ccggcttctt
    36601 cgagggcaat ctggttaaag gtcagttgat ttttgccttt accagcaatc tcttcaaaag
    36661 tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga cgcccggcaa
    36721 cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc agttcaatgc
    36781 cgtagttcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc agcgcctttt
    36841 catcttccag cagcggtttt agcaactcga gtgcacgctc gcgagagatt tgatcgggcg
    36901 catcaagata atcatgagca accggaatat atgccgctac gcctggctcg atagcaaaag
    36961 aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta tcaaatgcaa
    37021 ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca tcaaggatgg
    37081 tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg tctgcaacac
    37141 tggtttcctg tggcttcgcg gctggttttg cccctttggc ctgtaaccat ttgcccgctt
    37201 cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccccaac aactcttccg
    37261 ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaacg tcggttttaa
    37321 tcgtcgccag ctggtatgag agataagcaa cttctttgtt ttgctcgagc ttcgctgcca
    37381 ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca tacagcgtat
    37441 ccagtccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg cccggtacgc
    37501 caggaatgtt atcagaggag tcacccatca gcgccaggaa atcgatgatc agttctggcg
    37561 gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc atggtattga
    37621 taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg ctgatcagca
    37681 ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg tccgcttcta
    37741 cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc agaggttcga
    37801 tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt tcaaacagtt
    37861 catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc ggtttatatt
    37921 gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc ggctcgcctg
    37981 cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat gaaccatcta
    38041 caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt tatcagatta
    38101 tgggtaaagg atgccacaga caggataaaa acatgagttt ttcaccgctt ttcgtgaaaa
    38161 tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt ggataacttt gtgcataaat
    38221 tatttacatc gcttttaatt tattatcatg ctttttacag tatgatgttt gtctttgttt
    38281 ttcatgtagt taaaaataaa gccatcccta ataattgaga taatgggatt ttttctaatg
    38341 tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt cgttttctgc
    38401 taaaatctgc gccttgcgct tctttagcgc actgtctatg gctaaccttt tgaataaatt
    38461 cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat tgaccatttt
    38521 ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac tattgctacc
    38581 tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt attgctggtg
    38641 tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag ttcacgggct
    38701 ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta gttgggcaga
    38761 tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat atttcctcaa
    38821 gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg atatgccgtt
    38881 tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg gtaaagatgt
    38941 tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca ttgtcaattt
    39001 cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca cttttcaaaa
    39061 cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg gtaaacaatt
    39121 cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc cgttcttcga
    39181 tatgttaagc ggtaaactga cgcgaattgt cgttcatgtg gatttacagc ctattgccga
    39241 tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc agcaatggct
    39301 gaactcactg tggcaggaaa aagaccgact tctgacgtcg ttgatgtcat cacagcggca
    39361 ggacaagtaa attttgctcg cctctgaatg agcagaggcg agtgagtatt ttaatgaaca
    39421 aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt aaaaacaact
    39481 ttacccggtg tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt gattaactga
    39541 aacatctgac ctaaggtcat catcccgctg atttgtttat cagcccgctt ttgcgctttt
    39601 tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac gacattaagc
    39661 ggtaacttca catcaaaatt gagcgattta atatctttgt ttgtggatga tgaagatttg
    39721 gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc gttcttccat
    39781 tttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata ctctttgaac
    39841 aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg gtgtgcagca
    39901 agctgcttct gcattgcaat gttatactgg atgataaatt ggcgcaccgc tgagggatca
    39961 atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc ttttgttaac
    40021 ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac gcgatccagc
    40081 atggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt gataatgtta
    40141 atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc taatcgcgcc
    40201 agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa ttgcagaaca
    40261 atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc accattaaaa
    40321 gtaaactggc cctcaccaaa agaaaacttt ccatactctg caggggcaac atctaatgtc
    40381 gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga tttatttttc
    40441 gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt tgccgctgcc
    40501 agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc gtgctcaaca
    40561 tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc accgttatcg
    40621 aatgtcattt gcatttgaaa acgagaatta aaaaaacctt tgtcaaaatt tttataactt
    40681 aacgtcatat cgtaggcatg ttcacctttc tttttagcat cgttaaaatc tttaataaac
    40741 ttttcgacac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc ccagattacg
    40801 gctaacgcaa caataacacc tgtagctgac ttatgtatca tataaatatt ccctgataaa
    40861 acagcaaaac tttaaatatc cgcttatgct gaaataa
//
LOCUS       DAZFFI010000032        40479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFFI010000032 DAZFFI010000000
VERSION     DAZFFI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.032"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            263..349
                     /gene="leuL"
                     /locus_tag="ACHJ12_001999"
     CDS             263..349
                     /gene="leuL"
                     /locus_tag="ACHJ12_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4989789.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            442..2013
                     /gene="leuA"
                     /locus_tag="ACHJ12_002000"
     CDS             442..2013
                     /gene="leuA"
                     /locus_tag="ACHJ12_002000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4989790.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2013..3104
                     /gene="leuB"
                     /locus_tag="ACHJ12_002001"
     CDS             2013..3104
                     /gene="leuB"
                     /locus_tag="ACHJ12_002001"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4989791.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3107..4507
                     /gene="leuC"
                     /locus_tag="ACHJ12_002002"
     CDS             3107..4507
                     /gene="leuC"
                     /locus_tag="ACHJ12_002002"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4989792.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4518..5123
                     /gene="leuD"
                     /locus_tag="ACHJ12_002003"
     CDS             4518..5123
                     /gene="leuD"
                     /locus_tag="ACHJ12_002003"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4989793.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5172..6350)
                     /gene="setA"
                     /locus_tag="ACHJ12_002004"
     CDS             complement(5172..6350)
                     /gene="setA"
                     /locus_tag="ACHJ12_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO4989794.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6452..6583)
                     /gene="sgrT"
                     /locus_tag="ACHJ12_002005"
     CDS             complement(6452..6583)
                     /gene="sgrT"
                     /locus_tag="ACHJ12_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4989795.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6672..8327
                     /gene="sgrR"
                     /locus_tag="ACHJ12_002006"
     CDS             6672..8327
                     /gene="sgrR"
                     /locus_tag="ACHJ12_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4989796.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8361..8460
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8491..9474
                     /gene="thiB"
                     /locus_tag="ACHJ12_002007"
     CDS             8491..9474
                     /gene="thiB"
                     /locus_tag="ACHJ12_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4989797.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9450..11060
                     /gene="thiP"
                     /locus_tag="ACHJ12_002008"
     CDS             9450..11060
                     /gene="thiP"
                     /locus_tag="ACHJ12_002008"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4989798.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11044..11742
                     /gene="thiQ"
                     /locus_tag="ACHJ12_002009"
     CDS             11044..11742
                     /gene="thiQ"
                     /locus_tag="ACHJ12_002009"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4989799.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFGHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11844..12608)
                     /gene="yabI"
                     /locus_tag="ACHJ12_002010"
     CDS             complement(11844..12608)
                     /gene="yabI"
                     /locus_tag="ACHJ12_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4989800.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12694..13572)
                     /gene="araC"
                     /locus_tag="ACHJ12_002011"
     CDS             complement(12694..13572)
                     /gene="araC"
                     /locus_tag="ACHJ12_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4989801.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13911..15611
                     /gene="araB"
                     /locus_tag="ACHJ12_002012"
     CDS             13911..15611
                     /gene="araB"
                     /locus_tag="ACHJ12_002012"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4989802.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15622..17124
                     /gene="araA"
                     /locus_tag="ACHJ12_002013"
     CDS             15622..17124
                     /gene="araA"
                     /locus_tag="ACHJ12_002013"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4989803.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17293..17988
                     /gene="araD"
                     /locus_tag="ACHJ12_002014"
     CDS             17293..17988
                     /gene="araD"
                     /locus_tag="ACHJ12_002014"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4989804.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18063..20414
                     /gene="polB"
                     /locus_tag="ACHJ12_002015"
     CDS             18063..20414
                     /gene="polB"
                     /locus_tag="ACHJ12_002015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4989805.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20579..23485
                     /gene="rapA"
                     /locus_tag="ACHJ12_002016"
     CDS             20579..23485
                     /gene="rapA"
                     /locus_tag="ACHJ12_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4989806.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23497..24156
                     /gene="rluA"
                     /locus_tag="ACHJ12_002017"
     CDS             23497..24156
                     /gene="rluA"
                     /locus_tag="ACHJ12_002017"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4989807.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24273..25088)
                     /gene="djlA"
                     /locus_tag="ACHJ12_002018"
     CDS             complement(24273..25088)
                     /gene="djlA"
                     /locus_tag="ACHJ12_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4989808.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25343..27697
                     /gene="lptD"
                     /locus_tag="ACHJ12_002019"
     CDS             25343..27697
                     /gene="lptD"
                     /locus_tag="ACHJ12_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4989809.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27750..29036
                     /gene="surA"
                     /locus_tag="ACHJ12_002020"
     CDS             27750..29036
                     /gene="surA"
                     /locus_tag="ACHJ12_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4989810.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29036..30025
                     /gene="pdxA"
                     /locus_tag="ACHJ12_002021"
     CDS             29036..30025
                     /gene="pdxA"
                     /locus_tag="ACHJ12_002021"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4989811.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30022..30843
                     /gene="rsmA"
                     /locus_tag="ACHJ12_002022"
     CDS             30022..30843
                     /gene="rsmA"
                     /locus_tag="ACHJ12_002022"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4989812.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30846..31223
                     /gene="apaG"
                     /locus_tag="ACHJ12_002023"
     CDS             30846..31223
                     /gene="apaG"
                     /locus_tag="ACHJ12_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4989813.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31230..32072
                     /gene="apaH"
                     /locus_tag="ACHJ12_002024"
     CDS             31230..32072
                     /gene="apaH"
                     /locus_tag="ACHJ12_002024"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4989814.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32150..32629)
                     /gene="folA"
                     /locus_tag="ACHJ12_002025"
     CDS             complement(32150..32629)
                     /gene="folA"
                     /locus_tag="ACHJ12_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4989815.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32821..34683)
                     /gene="kefC"
                     /locus_tag="ACHJ12_002026"
     CDS             complement(32821..34683)
                     /gene="kefC"
                     /locus_tag="ACHJ12_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4989816.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34676..35206)
                     /gene="kefF"
                     /locus_tag="ACHJ12_002027"
     CDS             complement(34676..35206)
                     /gene="kefF"
                     /locus_tag="ACHJ12_002027"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4989817.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35314..36645)
                     /gene="yaaU"
                     /locus_tag="ACHJ12_002028"
     CDS             complement(35314..36645)
                     /gene="yaaU"
                     /locus_tag="ACHJ12_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989818.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36703..36990)
                     /gene="fixX"
                     /locus_tag="ACHJ12_002029"
     CDS             complement(36703..36990)
                     /gene="fixX"
                     /locus_tag="ACHJ12_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4989819.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(36987..38273)
                     /gene="fixC"
                     /locus_tag="ACHJ12_002030"
     CDS             complement(36987..38273)
                     /gene="fixC"
                     /locus_tag="ACHJ12_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4989820.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38324..39265)
                     /gene="fixB"
                     /locus_tag="ACHJ12_002031"
     CDS             complement(38324..39265)
                     /gene="fixB"
                     /locus_tag="ACHJ12_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4989821.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39280..40050)
                     /gene="fixA"
                     /locus_tag="ACHJ12_002032"
     CDS             complement(39280..40050)
                     /gene="fixA"
                     /locus_tag="ACHJ12_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4989822.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9759 a        11131 c        10629 g         8960 t
ORIGIN      
        1 taggttttgc aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt
       61 atgctgaaat atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt
      121 actttaacca ccgcagcaca attagctaat tttacggatg cagaactcac gctggcggga
      181 cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc
      241 gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact
      301 aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca
      361 gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta
      421 aaagagacaa ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc
      481 gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg
      541 gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc
      601 gattttgaat cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta
      661 gctcgctgcg tggagaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc
      721 ttccgtattc atacctttat tgccacttcg ccaatgcaca tcgccaccaa gctgcgcagc
      781 acgctggatg aagtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc
      841 gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga
      901 gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc
      961 tacaccatgc cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac
     1021 atcgacaaag ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac
     1081 tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc
     1141 gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat
     1201 attctcaacg tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt
     1261 agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc
     1321 gctcactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc
     1381 atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg
     1441 cgtgcggcgg tgaaacatcg catggatgag atggggtata aagaaagtga atataattta
     1501 gacaatttgt acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac
     1561 gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg
     1621 gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc
     1681 tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat
     1741 caggcaatta accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc
     1801 aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc
     1861 cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg
     1921 cacgtactga acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct
     1981 caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat
     2041 tgccggggga cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg
     2101 tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca
     2161 ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg
     2221 ccgtgctgtt tggctcggta ggcggtccga aatgggaaca tttaccacca gaccagcaac
     2281 cagaacgcgg cgcgctgttg cctttgcgta agcacttcaa attattcagc aacctgcgcc
     2341 cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta
     2401 acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa
     2461 aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt
     2521 ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag
     2581 tcacctcaat cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca
     2641 acgagatcgc cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca
     2701 ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg
     2761 gcgacattct gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg
     2821 ccagcctgaa cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata
     2881 tcgcaggcaa aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc
     2941 gctacagcct ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat
     3001 tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg
     3061 aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt
     3121 atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt
     3181 atatatcgac cgccacctgg tgcatgaagt gacctcaccg caggcgtttg atggtctgcg
     3241 cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt
     3301 ctctacccag accaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga
     3361 gctgattaaa aactgcaaag aatttggcgt cgagctgtat gacctgaatc acccgtatca
     3421 ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt
     3481 ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg
     3541 cacttccgaa gttgaacacg tactggcaac acaaaccctg aaacagggcc gtgcgaagac
     3601 catgaaaatt gaagtccagg gcaaagccgc gccgggcatt acagcaaaag atatcgtgct
     3661 ggcaattatc ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg agttttgcgg
     3721 cgaagcaatt cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga
     3781 aatgggcgca aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg
     3841 ccgtttgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct
     3901 gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc
     3961 accgcaggtc acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc
     4021 cgatccggct tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta
     4081 tatggggctg aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg
     4141 ttcctgtacc aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg
     4201 aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca
     4261 ggcggaagcg gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc
     4321 tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc
     4381 atccaccagc aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt
     4441 cagcccggca atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat
     4501 taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc
     4561 tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga
     4621 cccgtacggg ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc
     4681 aacagccaaa cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc
     4741 tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg
     4801 actacggttt taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct
     4861 ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc
     4921 tggtgaaagc caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag
     4981 cgggagagaa aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg
     5041 gtctggacag tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa
     5101 aacaacctgc gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat
     5161 cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata
     5221 accgcaatta cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata
     5281 acgccagcca gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc
     5341 gctcttccag gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata
     5401 aatacagcgt taaaaagttg cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat
     5461 ccggtgtaaa acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa
     5521 cgtttgacat agtagccagc cagaatcatt gctggtattt ccagtccagc tgccgtcccc
     5581 atcaggaaac ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata
     5641 tcaataatgt acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt
     5701 acgttactat cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc
     5761 cgcgccacag acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg
     5821 gcaatcgaaa acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga
     5881 ccgataaccc atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct
     5941 cgcgccgagt tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg
     6001 gccagagatg ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca
     6061 aacaatagcg cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg
     6121 ccctgactgt cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc
     6181 accgtataaa agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag
     6241 ctcaatgtag gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat
     6301 gccgcgtaaa caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt
     6361 tttagcgcgg cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca
     6421 ccaatactca gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact
     6481 gcatcagtcc ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca
     6541 acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt
     6601 catcgttagg aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga
     6661 gttccccttt tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct
     6721 gcgagggtaa atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc
     6781 gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag
     6841 cggaagtcgg gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc
     6901 agcaacagcg ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg
     6961 ttggcgacaa agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc
     7021 aggggcggca catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca
     7081 gcgcattgcg ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca
     7141 taaatgagga aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac
     7201 cgcttcactg gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg
     7261 aaatggacga cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata
     7321 ttgcggacat tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc
     7381 gctggttacc gttactgctg ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa
     7441 ccctcagtaa ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca
     7501 ccaccaatca actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg
     7561 atgaagttaa cgtctgggtt ctgccggaaa ttgccgacga gccagctgga ggtctgatgc
     7621 taaaaggtcc acagggcgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact
     7681 atttactgtt cgacagccgc acccatcgcg gggcgaatca gcaagtcagg gactgggtaa
     7741 gctatgtgct ttctccaact aatctggtct atttcgctga ggaacagtac cagcaactgt
     7801 ggttcccggc ttatggactg ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa
     7861 aaccggctgg cctggaaagc ctcaccctga ccttttatca ggatcacagt gagcatcggg
     7921 tgattgccgg gatcatgcag cagattctgg caagtcacca ggtcacgctg gaaatcaaag
     7981 agatcagcta cgatcagtgg catgaaggag agatcgagag cgatatctgg cttaatagcg
     8041 ccaactttac gctgccgctg gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc
     8101 tccaacactg tattccgatc gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa
     8161 tgaacctggc gaactggtgc cagcaactgg tcgccagtaa agcaatggtg ccgcttatcc
     8221 accactggct gatcattcag ggacaacgca gtatgcgcgg cctgcgtatg aataccctcg
     8281 gctggttcga ttttaaatca gcgtggtttg cgccgccgga tccatgattg ctggtagata
     8341 acaaaatcac tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga
     8401 aaatacccgt cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa
     8461 gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg
     8521 ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc
     8581 gccgccgact gggggcctgg tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc
     8641 gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa
     8701 ggcaaaaata gtaaagccga tgtggtgctg gggctggata acaatctgtt agacgccgcc
     8761 agtaaaaccg gactatttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc
     8821 ggctggaata atgacacttt cgtcccgttt gattacggct atttcgcctt cgtctatgac
     8881 aagaacaaac tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag cgatcaaaac
     8941 tggcgggtga tttatcagga tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg
     9001 atgcaaaaag tctatggcga tgacgcccca caagcctggc aaaaactggc gaagaaaacc
     9061 gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat
     9121 ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac
     9181 tacgccgccg cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc
     9241 gctgccagca agcagccgga gctggcgcaa aaattcctcc agtttatggt ttctccggct
     9301 tttcagaatg cgatcccaac cggtaactgg atgtatccgg tggcaaatgt cacgctgcct
     9361 gccgggtttg aacaattgac caaaccggcc accacgctgg agttcacacc agctgaagtg
     9421 gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg
     9481 gctggttaat tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt
     9541 ttctcgccct gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg caggacagct
     9601 acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctactatctg
     9661 tcgtacccgc gatattcctc gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg
     9721 cgctgttgcg tctgtgcgca atgaccttga tcctcccggt gctagttgct gttttcggca
     9781 ttcttagcgt ctatggtcgt cagggctggc tggcatcgct ctgccaatcg ctcggtctgg
     9841 agtggacctt ttcgccctac ggcctgcaag gcattttgct ggcgcacgta ttttttaatc
     9901 tgccgatggc gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc
     9961 agcttgccgc ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt
    10021 ggctacggcg acaaatcccg ccggttgccg cgcttatctt tatgctctgt ttcgccagct
    10081 tcgccaccgt gctatcgctg ggcggcggtc cgcaggcaac tactatcgag ctggctattt
    10141 atcaggcgct gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgctccaga
    10201 tggtgtgttg ccttgggctg gtgctgttga gtcagcgact aagtaaggcc attgcgcccg
    10261 gtaccacgct gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg
    10321 acacggtgtt aatagtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg
    10381 tcgatggcgt aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc
    10441 tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga
    10501 tgctgctatg gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc
    10561 tggagatgag cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct
    10621 ttttactgct caacaacact atcggcctgc cacaatctgc tgacggcatt gtgattttca
    10681 ccaatgcgtt aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata
    10741 tcaccgcccg ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa
    10801 aagtggtcga gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg
    10861 tgctgtcgat tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc
    10921 tgccgtttta tctctaccag caaattggct cctatcgcag ccaggacggc gcggtcaccg
    10981 cgttaattct gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc
    11041 gaaatgttaa aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc
    11101 ttaacggtag aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt
    11161 accctgctga atttgattgc cggttttctg acgccagcca gcggttcgct gactatcgac
    11221 ggcgttgatc acaccacgac gccgccgtca cgccgtccgg tatcgatgct gtttcaggag
    11281 aacaacctgt tcggccacct gacggtcgcg cagaacatcg ggctggggct taatccggga
    11341 ttgaagctga acgcggcgca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt
    11401 gataatttaa tggcgcggtt accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg
    11461 ttagcgcgtt gtctggtgcg cgaacagccg attttattgc tcgatgaacc gttctctgcg
    11521 ctcgatccgg cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa
    11581 aaaatgacgc tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc
    11641 tcggtggtgg tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc
    11701 ggtaaggcga gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg
    11761 gcgtgaacgc cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggca
    11821 agcgtcgcat caggcaaccc gccctaaacc ccaaccactt tacgcaaaat atcgatatac
    11881 accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat ggcagaaatc
    11941 aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg gtcagtcgct
    12001 ttaccgctgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa taccgctgtt
    12061 gccagcagca accatttaaa ctcaccgctc tgcattccgg caggaatatc gatcgccgcg
    12121 cccgccagaa tccccggcag gaagtaaaac ggcggccata acaggcagcc aataatattt
    12181 ggcgtgataa attttgccac cggcagatcc agcattcccg ccaccattgg caccagcgga
    12241 cgcgtcgggc caacaaagcg accgaccaga atggtgaaca tgctgtgttg atgcaacgca
    12301 tgttcagttt tatcgagtaa tgctttgttt ttcttcagaa atgaccagcg atgcaacggc
    12361 tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca accgacgatc
    12421 cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc caatcagcgc tcccagcccc
    12481 gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc
    12541 accaacacca ccgccatcaa tgaatacacg gtagattggg taataaagtg ttccagcaat
    12601 gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
    12661 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
    12721 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
    12781 tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
    12841 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
    12901 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gtgacaagca
    12961 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
    13021 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
    13081 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
    13141 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
    13201 ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa gccattcatg
    13261 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
    13321 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
    13381 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
    13441 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
    13501 cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg gatcattttg
    13561 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
    13621 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac
    13681 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
    13741 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
    13801 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
    13861 gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg atggcgattg
    13921 caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgccagcg
    13981 gtgaagagat cgccaccagc gtagagtggt atccccgttg gcaaaaaggg caattttgtg
    14041 atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg
    14101 cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg
    14161 gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac gtgctggcgc
    14221 tgcgcccgga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa gaccacactg
    14281 cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac gttgactact
    14341 cccgctacat tggtggtatt tattccagcg aatggttctg ggcaaaaatc ctgcatgtga
    14401 ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg
    14461 tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg
    14521 ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc agtttctttg
    14581 atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact gacacctgga
    14641 ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc ggcctgcctg
    14701 aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt ggcgcaggcg
    14761 cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt ctgattgccg
    14821 acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt gatggcagcg
    14881 tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat atctacgcct
    14941 ggttcggtcg cgtactcagc tggccgctgg aacagcttgc cgcccagcat ccggaactga
    15001 aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa gcatgggcca
    15061 aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt cgtcgcacac
    15121 cgaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct accgacgctc
    15181 cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca atcatggagt
    15241 gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc atcgcgcgga
    15301 aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg
    15361 cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc
    15421 acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac
    15481 cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag caatgggcga
    15541 tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc caggccgttg
    15601 cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg gtttgtcatt
    15661 ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac
    15721 gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt gaaaccgctg
    15781 ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga cgatcgttgc
    15841 gctggtctgg tggtgtggct gcacactttc tccccggcca aaatgtggat caacggcctg
    15901 accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc gctgccgtgg
    15961 gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg tcgcgagttc
    16021 ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca ctggcaggat
    16081 aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa acaggatacc
    16141 cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt caccgatggt
    16201 gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc ggttggcgat
    16261 ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt cgatgagtac
    16321 gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg acagaacgtg
    16381 ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg tggcttccac
    16441 gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg tctggccgta
    16501 cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa aaccgccgcc
    16561 ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc ctttatggag
    16621 gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca tatgctggaa
    16681 gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca tctcggtatt
    16741 ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc agcgattgtc
    16801 gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat cgacacggtg
    16861 aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa agcgcaaccg
    16921 gatctgccaa ctgcttccga agcgtggatc cttgctggtg gcgcgcacca taccgtcttc
    16981 agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga cattgaaatc
    17041 acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg ctggaacgaa
    17101 gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct gatgcgacgc
    17161 tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc aaagcgcatc
    17221 cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg aaacgacctc
    17281 tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa cctggcgctg
    17341 ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga tcgcgagcgc
    17401 ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc tgacgatatg
    17461 gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
    17521 acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat tgtgcatacg
    17581 cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc aatcggcacc
    17641 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
    17701 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag
    17761 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc gtttgcatgg
    17821 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
    17881 gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
    17941 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
    18001 accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga tggattttca
    18061 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg caagggacag
    18121 aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt gcaccgcaag
    18181 agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg
    18241 aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
    18301 gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg cgtgaaggtg
    18361 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
    18421 tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt aatgcccgtc
    18481 tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat attgaaacca
    18541 cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc atcgtttata
    18601 tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa tacgtcgcca
    18661 gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat cccgatgtga
    18721 tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt
    18781 accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc gagcacggct
    18841 ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac ggtatcgagg
    18901 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
    18961 tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa attgaccgcc
    19021 gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc gagctggtga
    19081 cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca acggtgaacg
    19141 gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc tattttccgc
    19201 gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccaccg cacgccagcc
    19261 ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg gtgctggact
    19321 ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggttg
    19381 aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat gcctggttct
    19441 cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgatgaag
    19501 ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg aatgcctttt
    19561 atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca tcgtcgatca
    19621 ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg cagggctacg
    19681 acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca cattcggaag
    19741 aagaggcggc gaaaatcggt cgtgtactgg tgcagcacgt taacgcctgg tgggcggaaa
    19801 cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact catttctgcc
    19861 gttttctgat gccaaccatt cgcggagccg ataccggcag taaaaagcgc tacgccgggc
    19921 tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg
    19981 actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc ttccgcaacg
    20041 agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt gaactggatg
    20101 cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag cgtaatgtgc
    20161 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgc ggtcgcccct
    20221 tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc ccggagccgc
    20281 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
    20341 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
    20401 ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
    20461 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac accactacct
    20521 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
    20581 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
    20641 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
    20701 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgatactat
    20761 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
    20821 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
    20881 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga
    20941 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
    21001 tatgccgtac agcggcctgc gcggtcagcg taccagcctg attccgcatc agctcaacat
    21061 cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt
    21121 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
    21181 acgtgtgcta attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
    21241 ccgtttcaac ctgcgctttg cgctatttga tgatgagcgt tatgccgaag ctcagcacga
    21301 tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
    21361 tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
    21421 agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg ccattgaaca
    21481 actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac agctggggat
    21541 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
    21601 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
    21661 aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg gcgagcagga
    21721 tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
    21781 ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
    21841 gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaaac tgccgctacc
    21901 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
    21961 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
    22021 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
    22081 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
    22141 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
    22201 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
    22261 gtgctcagaa atcggttctg aaggacgtaa cttccagttc gccagccaca tggtgatgtt
    22321 tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg
    22381 ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc agtcggtgct
    22441 ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga ccggacgcac
    22501 tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg atcaaaccga
    22561 aggctttgac gatctgatca aaaactgccg cgagcaacat gaagcgctga aagcacagct
    22621 ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
    22681 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
    22741 cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
    22801 gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
    22861 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta cctgggagca
    22921 tccgctgatc cgcaacggtc tggatctgat tctttctggc gataccggta gcagcacgat
    22981 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttatgt
    23041 ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
    23101 acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt ttgaaacctt
    23161 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acgccgtgca
    23221 gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc
    23281 attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
    23341 ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
    23401 caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg
    23461 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
    23521 ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt ggtcaacaag
    23581 ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag cgtgatgacg
    23641 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
    23701 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
    23761 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa
    23821 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
    23881 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc ggcggataac
    23941 acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg tgtgcatatg
    24001 ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
    24061 atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
    24121 aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg cagttcggta
    24181 ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg
    24241 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
    24301 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
    24361 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggca
    24421 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
    24481 agcgttgggc cacgctgcgc ttgctgccag ttaccaccgc cagtttgctg ctgataaccg
    24541 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    24601 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    24661 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    24721 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc tgattttccc
    24781 acccggaacg cattttgcgc cgcagtacgg gaagcgccat gaagattcat tcggtccatc
    24841 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    24901 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
    24961 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    25021 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    25081 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    25141 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
    25201 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    25261 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    25321 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    25381 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    25441 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    25501 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    25561 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    25621 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
    25681 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    25741 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta aagcggacct
    25801 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
    25861 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    25921 acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga tcttttatag
    25981 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    26041 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    26101 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    26161 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    26221 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
    26281 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
    26341 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    26401 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    26461 aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg cagagccgca
    26521 gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    26581 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    26641 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
    26701 gctggcaacc cattatcagc aaactaatct tgactggtat aactccagaa acacgaccaa
    26761 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
    26821 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    26881 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact catctctgct
    26941 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    27001 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    27061 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    27121 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgatactta
    27181 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    27241 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    27301 gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta agtactattc
    27361 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gctggccaat
    27421 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    27481 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    27541 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    27601 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    27661 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    27721 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    27781 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    27841 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    27901 tgaacgctgc tcaggcaagg cagcaacttc ctgatgacgc gacgctgcgc caccaaatca
    27961 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    28021 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    28081 tggatcagat gcgcagccgt ctggcttacg atggactgaa ctacaacacc tatcgtaacc
    28141 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    28201 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    28261 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    28321 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    28381 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    28441 tgggctgggg ccgtattcag gagttacccg ggatcttcgc ccaggcatta agcaccgcga
    28501 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    28561 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    28621 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg tgtgaaactg gaacagattg
    28681 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctcgcagg
    28741 atccaggttc tgctaaccag ggcggcgatc tcggttgggc aacagcggac atttttgatc
    28801 cggctttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    28861 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    28921 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg gaagaagcag
    28981 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    29041 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    29101 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    29161 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgcactt attcccccaa
    29221 ctctcctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg cgctacgtga
    29281 atctgtcact gcggggcagt tagcggttga aaatgggcat tatgtggtgg aaacgctggc
    29341 gcgagcgtgc gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    29401 taaaggcgtc attaacgacg caggcattcc gtttaccggt cataccgagt ttttcgaaga
    29461 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    29521 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cgcttttgca
    29581 cgaagtgatt gctattttgc atcacgattt gcggaccaaa tttggtattg ccgaaccgcg
    29641 cattctggtc tgcgggctga atccacacgc gggcgaaggc ggtcatatgg gtacggaaga
    29701 gatagacacc attattccgc tgctcgacga gttgcgggcg caggggatga aacttaacgg
    29761 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    29821 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    29881 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    29941 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    30001 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    30061 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    30121 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc attgaccgag
    30181 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    30241 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    30301 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    30361 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    30421 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    30481 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccagtg
    30541 ctggaagtac cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    30601 ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgtgtgtt gagccgcatc
    30661 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    30721 agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga aaatatctct
    30781 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    30841 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    30901 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    30961 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    31021 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    31081 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    31141 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    31201 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    31261 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct gggaaagata
    31321 ccctctggct gacgggcgat ctggtcgcgc gcgggccggg ttcgctggat gttctgcgct
    31381 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    31441 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    31501 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg ctgcaaatcg
    31561 acgaagagaa aaagttggtg atggcccacg cagggatcac gccgcagtgg gatctacaga
    31621 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc gagtgactcc tatcccttct
    31681 ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    31741 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttt ccaaacggtc
    31801 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    31861 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    31921 tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    31981 ggtcattaac ctgcctgcgc tgggaagata agcagtattt tgtccagccg tcgaaccggc
    32041 ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatatagga aggccggata
    32101 agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaaat taccgccgct
    32161 ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg aattcgctga
    32221 ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg
    32281 cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca taaacgcgac
    32341 cgccgccaat caccatgatt tctggtacgt caccacacgc cgcgatggct tcatccaccg
    32401 acttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc
    32461 gtcctggcaa cggacgaccg attgattccc aggtatggcg gcccataatc acgggtttat
    32521 ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt
    32581 ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc
    32641 gataaaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga
    32701 tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctctccc tggactcgcc
    32761 gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttacg tcactcttta
    32821 ttaggatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt taccttcttc
    32881 ggttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct cggtaatgat
    32941 ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg tatcattctc
    33001 aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat cggcacgttc
    33061 tcgcgcttca tacggcccca gacctaaact ttccagtgcc agacgcccgg ttttcagcgc
    33121 accttcgaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg
    33181 gtcgacatcg cgggcgcggg caataatctg caaatgcggg aaatgttctt tcaccatctc
    33241 tgtcagttgc aggttggttt gcggatcgtc gatggcgtta atcagcactt ccgctttcgc
    33301 cgctccggca gattccagta aatccatccg cgtggcatcg ccataaaaca ctttcatacc
    33361 aaatttacgc aaggtttcga tatggtccgg atcgtgatcg agtaccacca ttttcacccc
    33421 gctggagagc agtaaacgtc cggtaatctg cccaaaacga ccgaatccgg cgataatcac
    33481 gcgcggctgt tcttcgtcga tctcatcggc ttcacgcgct tcctcagtag aagattgctc
    33541 aaggcgattg aggatcacca gcagaatcgg cgttgctgcc atcgacagcg ccaccgccag
    33601 ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg cgccaaatac
    33661 cacaaaggca aactcactgc cctgccctaa caacaccgca aaccaacgac gctgtttatt
    33721 tggcacttgc aacggtcggg caatcagcca cagcatggcg attttgatga tgaggaaacc
    33781 gagcagcaaa atgacaatgc gcaatgggtt ttcaagcagc gtgccaaagt ctatcgacat
    33841 gccaacaccg atgaaaaaca gccccaacag caaaccttta aatggttcga tatcgctctc
    33901 cagcgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg cgcccatcgc
    33961 catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacgagga ataacgccac
    34021 ggcactaaac acttcccgca agccagagcg ggctacaaaa cgcagcgccg gacgcgtgac
    34081 atagcgcccc agcaatacca ccagcaccag cgcacccgcc acttttaacg ccgagagagc
    34141 aaatgcgccc atcgtcgtcg aggcactgct cgttgccagt agcggaatca tcgccaccag
    34201 cgggatcgcc gcgatatcct ggaacagcag caccgcaaag gcactgcgac ccatttgcgt
    34261 caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgtagagg agagcgccag
    34321 cgtcatgccg atcaattccg cgacctgcca gcgcagccca agtaacatgc agaacagccc
    34381 cagcaggccg ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag
    34441 cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca gcaccacgcc
    34501 aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc cccacgggcc
    34561 aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac gtaccgcaat
    34621 gggtacaatc agcgctgccg aaccgagata aatcagcgcc tgaatcagcg tatggctatc
    34681 catgatgggc ctcctgccat tccagcagac gttgcttata gtgacgcgcc tgcccttcga
    34741 gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc
    34801 cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc ggatgcgcac
    34861 caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat
    34921 gcagcgccgt gccgccgtga ccgtaagccc agccgtgcga gaaaacttta tcgatccaaa
    34981 gtttgaggag cggtggaatg ctgtaccact gcatcgggtg ctgccagacg atcagatcgg
    35041 cgcgagacag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga tagagttgat
    35101 aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc cgtttattcg
    35161 catgggaatg atgcggatac ggatgcgcat aaattataag aatcataggt agcccgcgtg
    35221 attacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta tagtcaataa
    35281 tattgattca gaaaagagag aaatctgaac aatttacccc attctctgcc cgcggatcgt
    35341 catattgctg gtttgcgcca gtgacatccc tcgagtctcc ggggcaaacg ctacggaaat
    35401 caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga taccgtaatt
    35461 attgataaag atcggcagtg cccaggtcga aacaatggtg ccaatacggc ttaaggacat
    35521 aatcacgccc acggcagagg cgcggatatc tgtcgggaag agttcattag gatagagcca
    35581 ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca tcactaccag
    35641 ccagatcccc atatccggga tcagccccaa aaccgccagc gccagcgtca tcatggcaaa
    35701 gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg gcggaataca
    35761 gccgagcata aagaacaggc taatcaccac gttccccagt gccgcgtttt tgccaacccc
    35821 caatcccaac aaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca ttgggatcac
    35881 ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc gattaaacag
    35941 atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt cgccaaacag
    36001 ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc agcggggaga
    36061 ttcaggcagt tcgaatcgac caatcaaaat caacaaacag gggatcgccg cgctacccag
    36121 catccagcgc cagccgcctt ccacatcata aagccagtag ccgaccagat cggcgcaggt
    36181 cgcgccgaca taccacatgg cggcgataaa gctgatggaa aacgcccgct gacgggtact
    36241 ggagaactcg gtgatcattg aggtggcgat gggataatct gcaccgatga caatgccgat
    36301 aagtacccgc atcaccaaca gttcgacagg ggatgagaca aacatcgtcg ccaccgatat
    36361 cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt tatcggagat
    36421 atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcac ccagcaagcc
    36481 aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca cgccaatcat
    36541 taccagtaca taaccatcca gaaacggacc accacttccc cacagcaaaa tgcggcggtg
    36601 aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt attcctgttt
    36661 tttgcaatga gagtcagtgc ggcgttacgt atcaaaccaa catcagccgt aacggaactc
    36721 cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca gccccagaat
    36781 gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc cgtcatcctg
    36841 cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagcacct gtttatcagc
    36901 atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat tgacgcccag
    36961 tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat gccatccttg
    37021 attagattga tgaagcccac tttcttaccg tggcggagga ttttcttgcg catcagttct
    37081 ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag ctccgggtag
    37141 ccgctgaaca tgcgtgggtt atcaaggaac gccggtagtt tctggtacat gcgcatatcg
    37201 cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt actgaaatcg
    37261 tcgcttttca tcgctgaaag cacggttttt gctgcagctt ccccggcggc aatcgccaga
    37321 tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc gccggcaatc
    37381 aatacgccgt caccgaccag ctccggcagc atgttgatgc ctgcttccgg cactacgtga
    37441 gcggaatatt ccaccagctt gccacccgcg atcagcggtg caacggccgg atgttgtttg
    37501 aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg cagaccacaa
    37561 accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc catcaggcca
    37621 tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa ctgaaaacgg
    37681 tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc cacatccgtc
    37741 ggtttaacgc gttttgccat ccccaacttt tcggcaagga tggaattcac cccgtcagca
    37801 aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac gactttgcca
    37861 tcgcgctgta cgaggttatc gacacgaatc ccggtaatta actgcgcgcc cgcttcttcg
    37921 gcctgctcca tcaaccaggc atcaaattta ctgcgcaaaa cggagtaaga acgctgggat
    37981 ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc cgtcataaac
    38041 gcgagttttt catgggtgat caggcgttct acgggggcgg agtcggcgaa accaggaata
    38101 atgtgttcca gactgtgggc atagagacgc ccgccggtga cgttcttggc acctgcggaa
    38161 ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc
    38221 gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga tatcttcgga cataagagct
    38281 ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg
    38341 cggtcagcgc cggaaggatc ttcacggcgt cgccaacaat gccgtaatcc gcgtactgga
    38401 agatcggcgc atttttatct ttgttgatgg cgaaaatggt ttgcgatgca ttagcaccaa
    38461 ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt ttcagcatca
    38521 ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc gccaccggac
    38581 gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc gcaatgttct
    38641 ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg agatcgacgc
    38701 tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga gcctgccact
    38761 ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg aacgtgccgc
    38821 tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc gccagaccac
    38881 cgtaaaccat gtgtttcact gtcgctttac cgtcctgtac gctgacggtg ctggcatcgt
    38941 tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg ccgcgacggg
    39001 tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac
    39061 cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag acatgattag
    39121 cgccgagctg gattgcctgt acgccgtcgg catcattgag gacaaaggtg ttgatttgat
    39181 tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta tcgctgaata
    39241 cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt tgcgaagatt
    39301 ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc gctgacgttc
    39361 gcgctgtttc ggcgcagcaa cctgttgttc agaccaggcg tccacggcgt taaaaccgat
    39421 atccgccgcc gaccatacct ggactggctt tttcgccgcg ccgagaatgg ctttcatcga
    39481 aggaatttgt ggggagttga tatcagtgga aacagcaaca accgcaggca gcggaatgct
    39541 taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg ccgtcaggga
    39601 aataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca ccagcagacc
    39661 aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat caaagcctgc
    39721 tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg
    39781 gtcatcaatc accacaatca gttcatccgg gccgcgcgat agcacatctt tacgcccttt
    39841 ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct ctgctgcctg
    39901 ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta ttttggcatc
    39961 ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct gttcatcagg
    40021 cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga aggaaacgtc
    40081 gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa taaaacttta
    40141 atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt gttaagttcc
    40201 tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt caatattgct ttatggatca
    40261 ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca aactgattaa
    40321 taatcaaaat taacgagaaa agcgccctct attcacccga aacaaaaatg tgataacaat
    40381 cacagaatgc agcttattga atacccatta tgagttagcc attaacgcgt ccacgaggtt
    40441 aataataatt atattaaatg ttaacaaaaa taaaacaaa
//
LOCUS       DAZFFI010000033        38698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFFI010000033 DAZFFI010000000
VERSION     DAZFFI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.033"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..2426
                     /gene="pdeF"
                     /locus_tag="ACHJ12_002033"
     CDS             183..2426
                     /gene="pdeF"
                     /locus_tag="ACHJ12_002033"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4989823.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAILATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2465..4006)
                     /gene="ppx"
                     /locus_tag="ACHJ12_002034"
     CDS             complement(2465..4006)
                     /gene="ppx"
                     /locus_tag="ACHJ12_002034"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4989824.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4011..6077)
                     /gene="ppk1"
                     /locus_tag="ACHJ12_002035"
     CDS             complement(4011..6077)
                     /gene="ppk1"
                     /locus_tag="ACHJ12_002035"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4989825.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6248..6886)
                     /gene="purN"
                     /locus_tag="ACHJ12_002036"
     CDS             complement(6248..6886)
                     /gene="purN"
                     /locus_tag="ACHJ12_002036"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4989826.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6886..7923)
                     /gene="purM"
                     /locus_tag="ACHJ12_002037"
     CDS             complement(6886..7923)
                     /gene="purM"
                     /locus_tag="ACHJ12_002037"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4989827.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8248..8874
                     /gene="upp"
                     /locus_tag="ACHJ12_002038"
     CDS             8248..8874
                     /gene="upp"
                     /locus_tag="ACHJ12_002038"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4989828.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8960..10249
                     /gene="uraA"
                     /locus_tag="ACHJ12_002039"
     CDS             8960..10249
                     /gene="uraA"
                     /locus_tag="ACHJ12_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4989829.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10344..11045
                     /gene="hda"
                     /locus_tag="ACHJ12_002040"
     CDS             10344..11045
                     /gene="hda"
                     /locus_tag="ACHJ12_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4989830.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11183..11542)
                     /gene="arsC"
                     /locus_tag="ACHJ12_002041"
     CDS             complement(11183..11542)
                     /gene="arsC"
                     /locus_tag="ACHJ12_002041"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4989831.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11563..13026)
                     /gene="bepA"
                     /locus_tag="ACHJ12_002042"
     CDS             complement(11563..13026)
                     /gene="bepA"
                     /locus_tag="ACHJ12_002042"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4989832.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13239..14300
                     /gene="yfgO"
                     /locus_tag="ACHJ12_002043"
     CDS             13239..14300
                     /gene="yfgO"
                     /locus_tag="ACHJ12_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4989833.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14338..15186)
                     /gene="focB"
                     /locus_tag="ACHJ12_002044"
     CDS             complement(14338..15186)
                     /gene="focB"
                     /locus_tag="ACHJ12_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4989834.1"
                     /translation="MRNKFSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VIGWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15208..17220)
                     /gene="hyfR"
                     /locus_tag="ACHJ12_002045"
     CDS             complement(15208..17220)
                     /gene="hyfR"
                     /locus_tag="ACHJ12_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4989835.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17250..17663)
                     /gene="hyfJ"
                     /locus_tag="ACHJ12_002046"
     CDS             complement(17250..17663)
                     /gene="hyfJ"
                     /locus_tag="ACHJ12_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4989836.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMNFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17656..18413)
                     /gene="hyfI"
                     /locus_tag="ACHJ12_002047"
                     /pseudo
     CDS             complement(17656..18413)
                     /gene="hyfI"
                     /locus_tag="ACHJ12_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18410..18949)
                     /gene="hyfH"
                     /locus_tag="ACHJ12_002048"
     CDS             complement(18410..18949)
                     /gene="hyfH"
                     /locus_tag="ACHJ12_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4989837.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFEPTVTNKA
                     DLYTRATFHLQRCSRCERPFAQQKTVALAAELLAEQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(18959..20674)
                     /gene="hyfG"
                     /locus_tag="ACHJ12_002049"
     CDS             complement(18959..20674)
                     /gene="hyfG"
                     /locus_tag="ACHJ12_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4989838.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20664..22244)
                     /gene="hyfF"
                     /locus_tag="ACHJ12_002050"
                     /pseudo
     CDS             complement(20664..22244)
                     /gene="hyfF"
                     /locus_tag="ACHJ12_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(22249..22899)
                     /gene="hyfE"
                     /locus_tag="ACHJ12_002051"
     CDS             complement(22249..22899)
                     /gene="hyfE"
                     /locus_tag="ACHJ12_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4989839.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPMKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22911..24349)
                     /gene="hyfD"
                     /locus_tag="ACHJ12_002052"
                     /pseudo
     CDS             complement(22911..24349)
                     /gene="hyfD"
                     /locus_tag="ACHJ12_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24366..25313)
                     /gene="hyfC"
                     /locus_tag="ACHJ12_002053"
     CDS             complement(24366..25313)
                     /gene="hyfC"
                     /locus_tag="ACHJ12_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4989840.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKMFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25324..27341)
                     /gene="hyfB"
                     /locus_tag="ACHJ12_002054"
                     /pseudo
     CDS             complement(25324..27341)
                     /gene="hyfB"
                     /locus_tag="ACHJ12_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27341..27958)
                     /gene="hyfA"
                     /locus_tag="ACHJ12_002055"
     CDS             complement(27341..27958)
                     /gene="hyfA"
                     /locus_tag="ACHJ12_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4989841.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28211..28681)
                     /gene="bcp"
                     /locus_tag="ACHJ12_002056"
     CDS             complement(28211..28681)
                     /gene="bcp"
                     /locus_tag="ACHJ12_002056"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4989842.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28681..29253)
                     /gene="gcvR"
                     /locus_tag="ACHJ12_002057"
     CDS             complement(28681..29253)
                     /gene="gcvR"
                     /locus_tag="ACHJ12_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4989843.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29399..30277
                     /gene="dapA"
                     /locus_tag="ACHJ12_002058"
     CDS             29399..30277
                     /gene="dapA"
                     /locus_tag="ACHJ12_002058"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4989844.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGYFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30294..31328
                     /gene="bamC"
                     /locus_tag="ACHJ12_002059"
     CDS             30294..31328
                     /gene="bamC"
                     /locus_tag="ACHJ12_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4989845.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31541..32254
                     /gene="purC"
                     /locus_tag="ACHJ12_002060"
     CDS             31541..32254
                     /gene="purC"
                     /locus_tag="ACHJ12_002060"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4989846.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32408..32635
                     /gene="vapB"
                     /locus_tag="ACHJ12_002061"
     CDS             32408..32635
                     /gene="vapB"
                     /locus_tag="ACHJ12_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO4989847.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32635..33033
                     /locus_tag="ACHJ12_002062"
     CDS             32635..33033
                     /locus_tag="ACHJ12_002062"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO4989848.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33180..34043
                     /gene="ypfJ"
                     /locus_tag="ACHJ12_002063"
     CDS             33180..34043
                     /gene="ypfJ"
                     /locus_tag="ACHJ12_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4989849.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34058..36073
                     /gene="tmcA"
                     /locus_tag="ACHJ12_002064"
     CDS             34058..36073
                     /gene="tmcA"
                     /locus_tag="ACHJ12_002064"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4989850.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIAAPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36147..36845
                     /gene="ypfH"
                     /locus_tag="ACHJ12_002065"
     CDS             36147..36845
                     /gene="ypfH"
                     /locus_tag="ACHJ12_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4989851.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36926..37126)
                     /gene="ypfN"
                     /locus_tag="ACHJ12_002066"
     CDS             complement(36926..37126)
                     /gene="ypfN"
                     /locus_tag="ACHJ12_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4989852.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37154..38281)
                     /gene="dapE"
                     /locus_tag="ACHJ12_002067"
     CDS             complement(37154..38281)
                     /gene="dapE"
                     /locus_tag="ACHJ12_002067"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4989853.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38285..38641)
                     /gene="yffB"
                     /locus_tag="ACHJ12_002068"
     CDS             complement(38285..38641)
                     /gene="yffB"
                     /locus_tag="ACHJ12_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4989854.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9429 a         9955 c        10166 g         9148 t
ORIGIN      
        1 acatgataaa cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga
       61 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
      121 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      181 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      241 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      301 ccggtgaggt ttttctcttt tatctgcctc tggcactgat gatcagcatg atgatgtttt
      361 tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc
      421 tgggttttta cgaaacgcta tcattaacgg ctaattttat tatcattatc attctctgtt
      481 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
      541 taatttccca gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt
      601 tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc
      661 cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg
      721 tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt
      781 attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc
      841 tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
      901 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc
      961 gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca
     1021 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1081 ccagttatct ggtattctct tttattgtca attatatggc tatactggca actcgtcagc
     1141 gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa
     1201 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1261 gcatccctgg catggaaatg ctggttaaga actatggcat catgctgcgg attcaataca
     1321 agcaaaaact ttctcactgg ctgtcaccct tgctggaacc gggtgaagat gtttatcagc
     1381 tttcgggtaa cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattaccg
     1441 cactggatag ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc
     1501 agattggcgt cagctactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg
     1561 gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc
     1621 gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac
     1681 tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc
     1741 gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatca
     1801 gccccgatag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt
     1861 gggtcattga gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc
     1921 gttttgctat taatctgtct ccaacctcgg tatgtcaggc tcgttttcct gttgaagtca
     1981 gtcagttgct ggctaaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa
     2041 gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag
     2101 gctgccagat tgcgattgat gatttcggca ccggctacgc cagctatgca cggcttaaaa
     2161 atgtgaatgc cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata
     2221 gtctggatta tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg
     2281 tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg
     2341 attatatgca gggttatctt attggtaagc cgcagccgtt aattgatacg ttgaatgaaa
     2401 tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa
     2461 agcattaagc ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca
     2521 cgccttccca gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa
     2581 accagtcatg cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg
     2641 gcggtgtggt ggttgcctga cgttgattgt ttagtaatac gccaaggcgc aatagctgta
     2701 tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa
     2761 tcgctttacg gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac
     2821 ccggcaagtc actgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga
     2881 tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat
     2941 gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca
     3001 cccgtcgggc ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac
     3061 tacgcacatc ctgatgacgg aaacgtcctt ccatttcgta cagtacgcct tcgcgtaacg
     3121 ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg
     3181 ccagtccagg aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg
     3241 cgaaattacg gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta
     3301 tcccgtcttt ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag
     3361 cgcccattgc cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt
     3421 gtgctgccgc catacgagcg cgctgaaaat tctctttatt gatgaccccg ccaggaaaat
     3481 aaagctgggc aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat
     3541 tttcaccaat caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt
     3601 tttccggttg ggtatgttcc acgcccataa aaattagacg ggcttcttca ttaccggaaa
     3661 taatttcaat cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca
     3721 gcgcctgacg cagtgtatgg gtgccaacta tacagacgct ggcaggagaa aacccttgta
     3781 gccgttcggc aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca
     3841 tattatctgg ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct
     3901 gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga
     3961 ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt
     4021 tgttcgagtg atttgatgta gtcataaatc gccaactgcg cccgtacttt gcggcgattg
     4081 ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg
     4141 ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggt
     4201 gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag
     4261 acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca
     4321 acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac
     4381 attccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca
     4441 accaggcctt tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc
     4501 tgcgcgttgg cgatttcgcg gtctaccatt tcatataaca gacggcgaga gttttgcggt
     4561 gacaccatta aataatcaaa tgtcaccggg cggtatgggt tttcaataaa gttaaatacc
     4621 cgccgtactt cgttggtgat acgcgcatct gcggtcagca acgaatagtc agtataaaga
     4681 cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cgtaacgcac cacttcaccg
     4741 ttttcttttc gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag
     4801 ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttagc ttcttcgtcg
     4861 aaacgcgcct gtaactcaac caccacggtg actttcttac cgttatgtgc ggcgtggatc
     4921 atcgagtcga tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt
     4981 acgctcgggt cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtgtgataa
     5041 ggataataga gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct
     5101 ttatcaaacc aaatatggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg
     5161 acattgggga aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag
     5221 tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg
     5281 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatt
     5341 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
     5401 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcgagg
     5461 cagtaacgca gaatgttatc caaaagaatc atcggcttgc gtcgacgcgg tgcttctggc
     5521 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg
     5581 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
     5641 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
     5701 taatgacgca gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
     5761 tggttgcgcg ccatctccag caatagctcg ttgtagaggc cgtcgaattc ctgatcggct
     5821 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt
     5881 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
     5941 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
     6001 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
     6061 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
     6121 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
     6181 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     6241 cggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg
     6301 cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa
     6361 tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata
     6421 ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca
     6481 ccgatgtacc gtgctcttca tcaccatttt ccagcgcttg acgatgggtg tgtaatccgg
     6541 gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa
     6601 acgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt
     6661 cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga
     6721 gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat
     6781 tgctgaaaac tgcccgtacg gtgcctttaa ttttgttagt tttacaggcg tcaataattg
     6841 cctgtaaatt acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac
     6901 gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc
     6961 gtttagcagg gcgagggctt tgtccacttc cggagcaggc agggcgataa tcatcccgac
     7021 gccgcagttg aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca
     7081 gttgaacact tccggccact gccaggaaga ttcatcaatc actgcctgag tattatctgg
     7141 caatacgcgt ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac
     7201 atcgaccttt tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag
     7261 atgatcggct aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc
     7321 aagaattttg cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat
     7381 cagcacatcg ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc
     7441 aacgcagaaa cccgcgacat cgtaatcctc accgtgatac atccccggca tttctgccgt
     7501 ttcgccaccc accagtgaac agcccgattg cagacatcct tccgcgatac cgctgatcac
     7561 cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg
     7621 ctctgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt
     7681 gtcgtgacgt tttaagtcca ttgccagacg cagcttggta cctacgccgt cagtgccgga
     7741 aaccagcacg ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag
     7801 accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag
     7861 agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt
     7921 cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa
     7981 cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttgccca
     8041 ggcacgcggc gaaagaagac ttgtgccagg gtaaaggtta gttttcggat ggaataatct
     8101 tctttcataa ccatttgaat ataaaataac tttatctcaa atcgttatca ttttgactaa
     8161 agtcaacgaa aagaatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt
     8221 gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc
     8281 aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc
     8341 gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa
     8401 gtaactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt
     8461 accgttgtgc caattctgcg tgcgggtctt ggtatgatgg acggtgtgct ggaaaacgtt
     8521 ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta
     8581 ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca
     8641 atgctggcaa ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc
     8701 agcagcatca aagttctggt gctggtcgct gcgccagaag gtatcgctgc gctggaaaaa
     8761 gcgcacccgg acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga
     8821 tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata
     8881 aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca
     8941 taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact
     9001 tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct
     9061 ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac
     9121 gctgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc
     9181 ctttatttca ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt
     9241 tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg
     9301 ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct
     9361 ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc
     9421 agactccaaa accatcatca tctctattac caccctggcg gtcacggttt taggttccgt
     9481 gctgtttcgt ggtttcctgg caattatccc gattttaatt ggcgtgctgg tggggtacgc
     9541 gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc
     9601 gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc
     9661 ggcgttagtg gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa
     9721 aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaatg gcttgtcgac
     9781 cgtgatttcc ggcttctttg gctctacgcc aaataccact tacggagaaa acattggcgt
     9841 gatggcgatc acccgtgttt acagtacctg ggttatcggc ggggcggcga ttttcgctat
     9901 cctgctttcc tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat
     9961 gggcggcgtt tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat
    10021 cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat
    10081 catcggcgtc agtggcgcga aggtaaacat cggcgcggcg gagttaaaag gtatggcgct
    10141 ggcgaccatc gtcggtatcg gcttaagttt gattttcaaa ctgatcagtg tgctgcgccc
    10201 tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg
    10261 ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt
    10321 gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc
    10381 ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggccg
    10441 cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag
    10501 gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg
    10561 cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta
    10621 tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt
    10681 gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa acacgattgt
    10741 tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc
    10801 gactcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg
    10861 cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct
    10921 tgctgaagcg gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc
    10981 gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt
    11041 tgtagataag gtgtttatta tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt
    11101 taatccatag tattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct
    11161 tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac
    11221 caatccgcgc tttgccattc gcgaccacaa tcgggcgctc cattagcttc ggattatcca
    11281 ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat
    11341 aaagatcctc tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca
    11401 aatcgcgcag cgttgccgca tcggcgggtg tctcaaggta gagtaccact tctggctcca
    11461 cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt
    11521 agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg
    11581 cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg
    11641 caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag
    11701 tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag
    11761 cgcggcttcc gcctgtgcca gtaaatccca gccgttgctg tcatctttat tattaaaggt
    11821 gtagcgattc agaatattgg ccgcttcttg tggttgaccg ccttgtagat aagcgttcgc
    11881 caggttgagc tgcaacaccg gattggtgcg caaatcacgg gcatttttca ggcgattgat
    11941 tgcctcattg gctttgtttt gcccaagatc gatatcggta gccagatcga gataccatgc
    12001 gttgccaggt tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt
    12061 ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac gaacgtttcc
    12121 ttttgcccat tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc
    12181 cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcggtcgcat
    12241 ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcaataa
    12301 aatttccggc gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg
    12361 catcgcctgc ggatcgaatc ccgagcgttg cagcacctga ataccaatgc gatccgcttc
    12421 ctgttcattt tgctgggtga agctgatcat cccctggcgc gttcccgcca gtgtaccggt
    12481 cagcgctgcc atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc
    12541 gacccaggtc agcggcgcgc tgcgctgttg atcttccatc gctcgcgcca ggtgacgttg
    12601 ggtgacgtgg gagatttcgt gcgccattac tgaagccagt tggctttcgt tatcggaata
    12661 acggaacagg gcagagtgca gtaccacgtt gccgccaaag aaagcaaagg cgttaatttc
    12721 gtcgttgttg atcagaaaaa aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag
    12781 acgcattcct agtgaattaa tgtactgcgt taatagtgga tcattaatta acggcgcgct
    12841 gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt
    12901 gcttcctgcg gaggttccca tatccggcaa ggtgtctgca ctatcggcaa acgccggggc
    12961 tacctgacca atggtcatgg cagcaatgag ggttgcaacc aggttttttt tcaactgcct
    13021 gaacataacc tctatcctgt atttctggag atagtcattt gaccgatact gtgaaatatc
    13081 gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gtccactcaa atgacttttc
    13141 agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg
    13201 gatcgcaatc ccgctcttga ggttcaggga aggtctttat gctcgaaatg ttgatgcaat
    13261 ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg
    13321 gatttggcat tatctttttc tttagtggcc tgcttgctcc gctgttggtg gctattgtgc
    13381 tggcctattt gttggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct
    13441 gggcgacgtc gattgtattg gtggttttcg tcggtatatt gctattgatg gcgttcgtgg
    13501 tactgcctat cgcctggcag cagggcatct acttaattcg cgatatgccg gggatgctca
    13561 ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgttg atggatgcgg
    13621 gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg
    13681 tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc
    13741 tggtaccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg aacgccgttc
    13801 gccgggtgct gccgcgtaac cgtggactgg caggacaggt gtggaaggag atgaatcaac
    13861 aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtggtgggg attgccacct
    13921 ggctgggatt cttactcttc gggctgaact attcgctgct gctggcggtg ctggtcggct
    13981 tctcggtact tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg
    14041 cgctattcca gtttggtgca ggcacggaat tctggagctg cttcgcggtg tatctgatta
    14101 ttcaggcgct ggacggcaac ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc
    14161 atccgctggt gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg
    14221 tattcttcgc cattccgttg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg
    14281 ggcaaatcgc gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca
    14341 gggttcctga cgtaaataaa tagcccgata acaaatactc accagcactg caccgccgat
    14401 aatattaccc agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc
    14461 cggaaaattg tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg
    14521 aatggcaatg gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agccactggc
    14581 gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca
    14641 ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga
    14701 ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat
    14761 cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc
    14821 tgccagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aaccaataac
    14881 gccccgactt tttgccatca ccgtcattac cgacgaggtg aacaggctgg tgccgcaaac
    14941 tgccagcaaa ataaagccga gtgtaaagca aaggccgccc accagatggg ttaatgcctg
    15001 tgaagacggg gcatctgcga taacggaaag gtaaaaagta aatccgatcg ccataaacac
    15061 gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc
    15121 aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag agaatttgtt
    15181 tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga tccccagacg
    15241 ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg cgccacgggg
    15301 cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac gttcttcttc
    15361 gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc
    15421 ttcatttagc gtcggcagta aacggctttg tcggacatta agatgtaaat tcagactgtt
    15481 accgcgagtc agcagtaccg ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg
    15541 ccagtcccac gacatcaact ggcgtagcgc ctcggtcggg atggcgtcaa ttgaacgatt
    15601 catatggcgc gccatttttt gcgtgaaatg ctttgctaaa agagggatat cttccggacg
    15661 gtcgcgtagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga gatcgctgcg
    15721 aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa tgactctgac
    15781 attcaccggg atcgttctac tcccgccgag acgctcaatc tcccgttcct gcaatacgcg
    15841 cagcagttta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa acaacgtgcc
    15901 gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa acgccccttt
    15961 gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc agttgatttt
    16021 gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga tcacctcttt
    16081 gccggttccg gtttcaccgc aaatcaacac cgtactgtcg ctcttcgcca caatatctac
    16141 ctgctggagt aggtcttcca ttgcctggct ttgatagata atgtcaccga tgcccagatt
    16201 cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct gcaaatcggt
    16261 catgctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg
    16321 tagtagctgg cagttttcct cactaaagag agtggaagag gtatgcgcca gcaacaatgc
    16381 acccggtgta tggttgccaa aggtaagcgg tataaggagc gcagattcgc agccgttaga
    16441 tgcaagtaag agtaataacg ggtcgcgttg ccagagaaac agatcgtctg cacggtgcgt
    16501 caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt
    16561 gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc taaacttctc
    16621 gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat
    16681 ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt
    16741 aatatcaacc agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgccacctg
    16801 ctgttcttca tcattgagcc atgattgcag aaccggggtg attatccgcg ccagccagtt
    16861 aaacagtgtg caatcctggg gagtaaaacg gctgccgtgg gtacgaataa agcgtagctc
    16921 cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgacca tctcgccctg
    16981 atactctgtt gttgccgtca catcgctgcc atcatcatgg tagcggtaat agaagttgct
    17041 tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca tcgggatgaa
    17101 ggcgcgtaat aaagacgcct taccgtgttt ctgtgctaac cgctccgcga gcattccgtt
    17161 taccgcttca attgttattc cttgtggcgg ggcaaacatc gcctcgtctg acatagccat
    17221 caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat cagatagatg
    17281 gccggttcct gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcctgccac
    17341 acagacgtcg cgtcatccgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg
    17401 gcgtcaataa caatctcacc aaaattcatc acccccagca tcttacgtcg ggcttgctcc
    17461 tcttcgacct gggcaagcca atcttcgtat tccgtcagtg ggcaggggaa ggcgacattc
    17521 agacaatcaa tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgagag
    17581 ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca
    17641 atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt gctgaacgat
    17701 actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg
    17761 atcgtcgctt aaatggcgca ggagccgatc gcaaatttct cgcccctgac gatagcccgc
    17821 cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct gcggccacaa
    17881 cggttgcata gtcaccccgg tgggatcgcg ataatccacc gcgtgaatct tctgttgcag
    17941 caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg
    18001 gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg
    18061 gaaaatccgc cgccggcacc gcacgcgccg taggaaacac agattttatg atcgggggca
    18121 gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc tgtaaataac
    18181 aaaatatcgg cgtgacgcgg tgatgaaata accttaatgc caaaacgttc tgcgtcgaat
    18241 actggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc gcaatcgacg
    18301 cgataaacgt aagccgagcg acggatatcc tgtagcaaat gctgcttcat cttttgcgtt
    18361 tgctcgtcca gcgtgatggg ctggctgaca tattgtgtaa gcactggact catagctgct
    18421 ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt tgtttgcact
    18481 ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca ttttgttgct
    18541 ctgccaacaa ttcagcagcc agtgcgacgg ttttttgctg ggcaaacggg cgttcgcaac
    18601 ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta ttggtgacgg
    18661 tcggttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct tcacaacgtc
    18721 cgcagtaaat acaacgcctc agatagagct gccaggtgcg cgaattttgc tggtcgtcgg
    18781 tctggatagt cagcgcattt gccggacaag cacaggcgcc gcaggcaata cattggctgg
    18841 gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg aagggatatt
    18901 tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc ttctgtcctt
    18961 acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta tacggcacgg
    19021 tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag gagtagcagg
    19081 gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc agcatgtaac
    19141 gcaacaccgg ccagttggcg taggtggcgg cacgacagcg ccagcggaac aatttttggt
    19201 tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca aagcccagcg
    19261 cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag cggggtatcc ggcatgttgt
    19321 cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg accatcacgc
    19381 gggagaaaac atcgccgccg gtgaaggtaa acagtgtttt tggaatattg ccgtagtcgg
    19441 cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc agcggcccta
    19501 cagggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt
    19561 ccatattcgg catagcaagc agcatctcta ccagctccga cacgtcggcg cgcatctcgc
    19621 gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga acaccaccaa
    19681 tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatc gtcatcgact
    19741 tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa accaacaaaa tggcaggaga
    19801 ggccaaggtt gagcaaatga ctgtgtagcc gttcgacttc cagcagaatc gagcgaatgg
    19861 tatgcgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg gtataggcca
    19921 cactgtgggc aaaaccgcaa atcccacaca cgcggtccga taagaaggtc acttcgttgt
    19981 agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac aggcggtaat
    20041 cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccggt tcatcggagg
    20101 tgatatgcag cgggccgaca gggatcaccc gcgcatcgct gttgccctca ttgataaacg
    20161 catacgtttc ggaatctgtc gtcggttcag ggcgcagtcg ataatccatc gcatctttgc
    20221 gcagcggatg catatcttcc ggccagtcat cgggcaacac caggcgacgc tgatccggca
    20281 ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag accgcggcag
    20341 ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt gccttcacca
    20401 cgatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg taatgaccgt
    20461 taagtgtccg ctcgtcgttg ccaaacagga ccggaagcca gccatcatgt tgataataaa
    20521 gataatgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc tgttcaggcg
    20581 tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg
    20641 cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac agatgcggtg
    20701 cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag
    20761 aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat atgcgttccc
    20821 atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca gccgagatca
    20881 ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgcagccat ccgtaccagg
    20941 cccgccagca ccagcgttaa cagcaataac agcatgacga taatcagcag gtgattacgt
    21001 gccagtccgg cggtaacggt cataaattcg ctaagaaaaa tgttgaaggg cggcatcccg
    21061 gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa atggcatgat tttgagcatc
    21121 ccgcagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc ggaaccgcag
    21181 aacagcagcg tttttgccag actgtggttt aaggtgtgca gcagcgcggc aaaaattccc
    21241 agcggcccgc caatgcctag cgccaccgcg accagtccca tgttctccac gctggagtac
    21301 gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac ggcaaccgac
    21361 aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc ctggcagata
    21421 atgatgtaat agcgaatcat caccaacagc gcgcagttca gcaatacggc ggagagcagg
    21481 gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg cataggaaat
    21541 agcccggttt tggtaccaaa gccaattagc acaaacacaa aggccaatag cattaatgtt
    21601 gggtcaagca aggactattg cttaagaacc tcgctccaga atatcgccat ttctgcctgc
    21661 ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc aaaagcgacg
    21721 ccaacagtac aaataatgat gtacttccat gcagcttcca gcgatgaacg ctgaccgtaa
    21781 atgcctacca gaaacgccga gcttaaggtg gtggcttcga tcgccgccca catcacaatc
    21841 aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa gaaaccgtag
    21901 taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca cttcatgacg catgtagcca
    21961 atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa caaaccgccc
    22021 agaccatcaa tatgaagcca cagtcccgca gcgaatattt ctcccgcacc agcggcagtt
    22081 tgaaccaacc agagtgccag aatcagcagc agtgtgatcc ctaagctatg taatactgtc
    22141 accgcgcgag tcgcagagtg cccccgtttc cggcaggcaa aacagagcag cgaaaaaagc
    22201 agcggcgtga gcaggagtaa agcgaacatc acagaataac tcatctcatt atcccttcag
    22261 cgcggtcaag ttgttcacgt ccagcgtacc gtgggtacgc catatttttc ttgccagtaa
    22321 caccatcaca atgacggcga agatggcgtc ggtagcgata cctatttcca ccagttccgg
    22381 tgctcgccag gcaagaagcg ccagcaccag atgggagccg ttttccatca ggcagtaacc
    22441 aaaaatttgc cgcaggatat tgcgctggct gacaatgcac agcaggccaa gcagaaaatg
    22501 acctaacgct accgccagcg ccggtttcag cccggtagcc atcggtagct tcatgggctg
    22561 aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgccatca ttgccggacc
    22621 gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag tcatgattaa
    22681 cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca gttgctctgc
    22741 ggcgaacagg cacgagagag tggcgaaaat agacaccagc accagcgact ggcaggcgta
    22801 aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg tcagcatcat
    22861 cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc ttactgcaac
    22921 caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat caacaccagg
    22981 cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt gccaggcaca
    23041 acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat catcagcaga
    23101 atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccggc agcaaacagc
    23161 gggaatttac tgaagaagcc gttgaacggc ggcacaccgg taatcgccag cgctgccacg
    23221 cagaaaccca cgcctggcaa cggcagggtg tgcaatacgc cacgcagacg cggcaacaag
    23281 cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct tttagcgaac
    23341 gcgtggttga cgatgtaggc gatgctaccc tccagcgcca gccgcgagcc gaagatggag
    23401 agcgacaaac cgaagaacat ccagccaagt tgagtgatgg tcgaccaggc cagcaaccgc
    23461 ttcatatcct gctgtggcaa atacatcaga aagccataaa gaatggtgac cagcgccatg
    23521 accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat ggcgcgggca
    23581 aaaatgtaaa cgcccacttt caccttcgat gcggcgtgga gataggcact gatcggtgtt
    23641 ggcgcttcca ttgcatccgg tagccacgct tgcatcggta gctgggccga tttcccccac
    23701 gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccgtgtaa ctcgctcatc
    23761 gcgctaagca cacacgttcc ggtctgcaaa aacagcgtgg cggcggcaag atacaacccc
    23821 aacgaaccga tatgggtgat aagcaacgct tttagtgctg aacgctgtgc tttatcgctc
    23881 tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa caacaactga
    23941 ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacaa cagcaaaaat
    24001 gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt cagataaccc
    24061 gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat cagcgtactc
    24121 acgcgatcaa tgaccaggcc aaacaatgcc acatccccca cgttgaccaa cgtcaacgtg
    24181 acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca cagcgtggtc
    24241 agtgcggcga acaaaacccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    24301 gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt
    24361 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    24421 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    24481 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    24541 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    24601 atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat
    24661 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    24721 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    24781 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    24841 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    24901 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    24961 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    25021 ggggaaacgg taataaacag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    25081 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    25141 aacattttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    25201 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    25261 gcctgtgcta acgcaaaaat aaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    25321 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    25381 gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt gggtggcgct
    25441 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    25501 gacgtgcgca aaacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    25561 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gggccatcgc
    25621 attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    25681 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    25741 cagtaatgag ggggtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    25801 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    25861 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    25921 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    25981 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    26041 taccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    26101 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    26161 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    26221 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcacccgcgc cgagaaatag
    26281 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    26341 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgaccg ccagcaaaat
    26401 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    26461 tgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gagtgcccca
    26521 ccacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    26581 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    26641 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    26701 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    26761 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    26821 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    26881 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    26941 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    27001 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    27061 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    27121 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    27181 aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt gatgccggaa
    27241 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    27301 tagaggatca gcgaccaggt taataattgc aaggcatcca ttagtgttgc tcctgagtga
    27361 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    27421 tcatctgtcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    27481 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    27541 ggacacccgg ttcccagcgc agcaatgcat gttggggcgg cacgttttct tcgccgtctt
    27601 tgagtgagcc ttcagcctga aaaacatatt gcgcaggggc attcaccgga cggcttcctg
    27661 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    27721 tttcgttgag ttggatcgca tcatccctct gggagatggc attaaccggg cagacctgca
    27781 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt gacgtcttcg
    27841 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    27901 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    27961 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga tgccattctg
    28021 attaactcaa tatattcaat aagttatgta ttgactggat tctgaacggt acaaaaatga
    28081 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    28141 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    28201 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    28261 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    28321 gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    28381 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    28441 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    28501 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    28561 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    28621 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    28681 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    28741 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    28801 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    28861 ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    28921 gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag atgctggcat
    28981 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc
    29041 tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg aaagcagcat
    29101 aataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    29161 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    29221 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    29281 gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt ctgtctgctt
    29341 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
    29401 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    29461 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    29521 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    29581 gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    29641 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    29701 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    29761 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    29821 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    29881 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    29941 tgttctgctg agcggcgatg atgcgagtgc gctggacttc atgcaattag gcggtcatgg
    30001 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    30061 agcagaaggg tattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    30121 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    30181 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    30241 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    30301 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    30361 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    30421 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    30481 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    30541 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    30601 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    30661 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    30721 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    30781 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    30841 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    30901 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    30961 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    31021 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg
    31081 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    31141 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    31201 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    31261 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    31321 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    31381 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    31441 gtagttcgga taaggcgttc acgccacatc cgacaaaaca tccggcacac cagacagcaa
    31501 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    31561 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    31621 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    31681 tatggtgaac aacaagttca attacttcat tatgagcaaa ctggctgaag cgggtatccc
    31741 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    31801 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    31861 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgacgccat
    31921 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    31981 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    32041 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggcctgtaca aaggtgaagt
    32101 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    32161 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    32221 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tggttgcttg tttctttagc
    32281 cggagggtac tccggctaaa tgtctttgat acccccgcta caatacttct cattatctcc
    32341 ctttgacagg aaagtcatac caggccaata tgtatataca taacgtatat cccaaaaagg
    32401 gggggctatg gaaactactg tatttttaag taatcgcagt caggccgtca gattaccgaa
    32461 agcggtcgct ctgccagaag acgttaagaa agtagaaatt attgcgattg gtcgtacaag
    32521 aatcatcact cccgccggtg agtcctggga cagttggttt gatggcgaaa atgtgagtgc
    32581 ggattttatg gatatccgtg atcaacccgc aatgcaggaa agggaatcat tttgatgctg
    32641 aaattcatgc tggataccaa tatctgtatt ttcactatta agaataaacc agtacatgtc
    32701 agagagcggt ttaacctgaa ttctggccgg atgtgtataa gttctgtaac attgatggag
    32761 cttatctatg gggcagaaaa aagtcagatg ccagaacgca atttggcggt tattgaaggt
    32821 ttcgtatctc gtttggtagt gcttgattat gatagtgctg ctgcgacgca taccgggcaa
    32881 atcagggccg agcttgcccg gcagggaagg cctgttggac cttttgacca gatgatagcg
    32941 ggacatgctc gcagtcgcgg tttgattgtg gttaccaaca atacccgaga gtttgagcga
    33001 gtggcgggaa tcagactaga agactggagc taacattctc ttttacttct aacattcgta
    33061 aattatataa cgtggcatga agccttaatt cctccgccat ttcatcccgc tttcctgtgg
    33121 taatcttcct ctgaaccacc tacgatcatt tctataatga atatattgtt gaggtatcta
    33181 tgcgttggca agggcgacgt gaaagtgaca atgttgaaga caggcgcaac agctccggtg
    33241 gtccgtctat gggcggtccc ggttttcgcc tgccaagcgg taaaggcggg ctgattttac
    33301 tgttagtcgt gctggttgca ggctactatg gtgttgattt aaccgggtta atgactggac
    33361 agccggtttc ccaacaacaa tcaacgcggt ccattagccc taatgaagac gaagctgcaa
    33421 aattcacctc ggtgattctg gcaaccacgg aagacacctg gggacaacag ttcgagaaga
    33481 tgggtaagac ctatcagcaa ccgaagctgg tcatgtaccg tggaatgacg cgcaccggct
    33541 gcggggctgg tcagtccatt atggggccgt tctattgccc ggcggatggc acagtttata
    33601 tcgatctctc cttctatgat gacatgaaag acaaacttgg cgcggatggc gattttgccc
    33661 aggggtacgt tatcgcccat gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc
    33721 cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga agtgaaccgc ttatctgtgc
    33781 gtatggaact ccaggccgac tgttttgccg gtgtctgggg gcatagtatg cagcagcaag
    33841 gcgttctgga aaccggcgat ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg
    33901 accgtttaca acagcaaagt caggggcgag tagtaccaga tagtttcaca catggcactt
    33961 ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag cggcgatccg gcacaatgca
    34021 atacttttgg taaaagcatt taacttccgg ggcagggatg gctgaactga ctgcgcttca
    34081 cacattaaca gcgcaaatga aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga
    34141 agagggttgg tgttttgatc atgcgcttaa gttgcgtgat gccttacctg gcgactggct
    34201 gtggatttcg ccgcagccag atgctgaaaa ccactgttca ccctcggcgc tacaaacttt
    34261 acttgggcgc gagttccggc atgcggtatt cgacgcccgc cacggctttg atgccgctgc
    34321 ctttgcggca cttagcggaa cgttgaaagc gggaagctgg ctggttttgt tactccctgt
    34381 atgggaagag tgggaaaacc aacctgatac cgactcgctg cgctggagtg attgccctga
    34441 ccctattgcg gcgccgcatt ttgtccagca tttcaaacgt gtacttacgg cggataacga
    34501 cgctattctc tggcggcaaa accagccgtt ctcgttggcg cattttactc cccgtactga
    34561 ctggcacacc gcgaccggcg caccacaacc agaacaacag caactcttac agcagctact
    34621 gaccatgccg tcgggcgtgg cagcggtaac ggctgcgcgt gggcgcggta aatcggcgct
    34681 ggcagggcaa ctcatttctc gtattgcggg tagtgcgatt gtcactgcgc ccgcaaaagc
    34741 ggcaacggat gtactggcac aatttgcggg cgagaagttt cgctttattg cgccggatgc
    34801 cttgttagcc agcgatgagc aagccgactg gctggtggtc gatgaagccg cagccatacc
    34861 tgcgccgttg ttgcatcaac tggtatcgcg ttttcctcga acgttgttaa ccactacggt
    34921 gcagggctac gaaggcaccg gacgtggttt tttgctgaaa ttttgcgctc gctttccgca
    34981 tttacaccgt tttgaactgc aacagccgat ccgctgggca cagggatgcc cgctggaaaa
    35041 aatggttagt gaggcactgg tttttgacga tgaaaacttc acccatacac cacaaggcaa
    35101 tatcgtcatt tccgcatttg aacaaacgtt atggcgaagc gagccagaaa cgccgttaaa
    35161 ggtttatcag ttattgtctg gtgcgcacta ccggacttcg ccgctggatt tacgccggat
    35221 gatggatgca ccagggcaat attttttaca ggcggctggc gaaaacgaga ttgccggagc
    35281 gctgtggctg gtggatgagg ggggattatc tcaacaactc agtcaggcgg tatgggcagg
    35341 ttttcgtcgc ccgcggggta atctggtggc ccagtcgctg gcggcgcacg gtagcaatcc
    35401 actggcagcg acattgcgtg gacggcgggt cagccggata gcagttcatc cggcgcgtca
    35461 gcgggaaggc acagggcagc aacttattgc tggtgctttg caatatacgc gtgacctcga
    35521 ctatctttcg gtgagttttg gttacaccgg ggagttatgg cgtttctggc accgctgcgg
    35581 ttttgtgctg gtgcggatgg gtaatcatcg tgaagccagc agcggttgct atacggcgat
    35641 ggcgctgtta ccgatgagta atgcgggtaa acaactggct gaacgtgagc attaccgttt
    35701 acgtcgcgat gcgcaagctc tcgcaaagtg gaatggcgaa acgcttcctg ttgatccact
    35761 aaacgatgcc gtcctttctg acgacgactg gcttgaactg gccggttttg ctttcgctca
    35821 tcgtccgcta ttaacatcgt taggttgctt attgcgtctg ctacaaacca gtgaactggc
    35881 attaccggcg ctgcgtgggc gtttacagaa aaacgtcagc gacgcgcagt tatgtaccac
    35941 acttaaactt tcaggccgca agatgttact ggtccgtcag cgggaagagg ccgcgcaggc
    36001 gctgttcgca cttaatgatg ttcgcactga gcgtctgcgc gatcgcataa cgcaatggca
    36061 attttttcac tgactccttc aattttctcc catggtcatt gtgagtcagc ggcgtaaaat
    36121 taatgacatc agttaaggag ttcgccatga aacatgacca ttttgttgtt caaagcccgg
    36181 ataaacctgc gcaacagttg ttactgcttt tccacggtgt cggggataac ccggtggcaa
    36241 tgggggaaat aggtagctgg tttgcaccgc tgtttcctga tgcgctggtg gtgagtgtcg
    36301 gtggcgcgga gccgagcggt aatccggcgg ggcgtcagtg gttttcagtg cagggtatta
    36361 cggaagataa tcgccaggcg cgtgtggatg ccatcatgcc gacgtttatt gagacggtgc
    36421 gctactggca gaaacagagc ggagtagggg ctaacgccac ggcgttaatc ggtttctctc
    36481 aaggcgcgat tatggcgctg gagagcatta aagccgaacc gggccttgct tcacgcgtca
    36541 ttgcttttaa cggacgctat gcgagcctgc cggaaactgc gtctacagcc acgacaattc
    36601 acctgattca tggcggtgaa gatccggtta tcgatctggc gcatgcggtt gcggcgcagg
    36661 aagcgttaat cagtgccggt ggtgacgtga cgctggatat cgtggaggat ttaggccatg
    36721 cgatcgacaa tcgcagtatg caatttgccc tggatcactt gcgttatacc attccgaaac
    36781 attatttcga cgaagcgcta agtggcggta agcctggcga tgatgatgtg attgaaatga
    36841 tgtaataata gccgggtagg cctgataaga ctgtatcagg catcatggcc ggatgtggcg
    36901 tgaacgcctt atccggccac tcagcttact tcttcggttg atcctttttc ggccagtcgt
    36961 cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg aagctccggc ttgttggcga
    37021 gaaatttctt atggtcgacg cgcttgagat ctttaatcac gttcagcagg acgcctacca
    37081 aaaagacaat caccagaatc caccaatatt tagccagcca gtccatgctt atttcctctt
    37141 gcagaaccac tcatcacgcg acgagctgtt ccatgatacg ttgatacata cgggcaagta
    37201 gctgcaggtc ggcagcgttc acacattcat taattttatg aatagtggca ttgaccggcc
    37261 cgagttccac cacctgcgcc cccatgcggg caataaagcg cccgtcggac gttccgcctg
    37321 tggtcagtag ctgcggttta atttcattat agtgctcaac cgcgttaacg accgcatcca
    37381 ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag ccaccagtcc accgtataac
    37441 gcagttgatg tttttcaagc agggcaagca cctgcgcttt gatcatctca tcagtcagtt
    37501 cggtgctgaa gcggaagtta aactgcacaa acagttcacc cggaataacg ttgttactgc
    37561 ccgttcccgc ctgaatatta gcaatctgca tactggtcgc cgggaaaaat tcattgccct
    37621 gatcccactc aatagccact aattcattaa ggaaaggtgc tgcgcgatgt actggattgt
    37681 cagccagatg cgggtaggcc acatgcccct gaacgccatg aatggtaagg ttgcaggtta
    37741 acgatccgcg acgaccattt ttcaccacat caccaaccac ttcgatactc gacggttcgc
    37801 cgaccaggca gtaatcgaga cgctcattac gcgacattaa cgcttcgacg acttttaccg
    37861 taccgttgtg ggcactagct tcttcatcag aagtgatcag aaatgccagt cgccccgtat
    37921 ggttgggatg ttgtgcgaca aaacgttctg ccgctaccac catcgccgcc agcgagcctt
    37981 tcatatctgc cgcaccgcgc ccgaataaca tgccgtcacg aatggttggt tcaaacggcg
    38041 gattgatcca acgatcggcg tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcta
    38101 acgtttcacc ctgcccacgc catgcccaaa aattctgcgt atcggcaaag tccatgcgtt
    38161 caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa agcctggcat cctgcgtcat
    38221 caggactcag ggaagggcgg cgaataagct gttgtgtcag ctcaataacc gggcacgaca
    38281 tagactacac ctcatggaaa aattgctgat aactggaatc actgaaaccc agcagcatag
    38341 gcttaccagg cgcgcagagc aatggacgtt tgataattgc aggcatttca gtcattaatg
    38401 ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt ttacgccagg
    38461 ttgtaccacg ggtgttgagt aacgcttccc agcctaattc gttgataaaa ccgttcaata
    38521 attcgctgtc cagcccatcg acgcggtaat catgaaaacg atagtcgatg ttattggctt
    38581 ccagccaacg gcgagccttt ttaatggtgt cacaattttt gatgccgtaa agtgtaacca
    38641 taatgaatcc tttttcgatt tatgagaata aatactcatt taagggtgaa aacatttt
//
LOCUS       DAZFFI010000034        38577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFFI010000034 DAZFFI010000000
VERSION     DAZFFI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.034"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..381)
                     /locus_tag="ACHJ12_002069"
     CDS             complement(<1..381)
                     /locus_tag="ACHJ12_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFO4989855.1"
                     /translation="MEITKNKNATIKKQSSVGCFSMFTLSFSITFIPAFLTNRQELIS
                     RGLLVPALFLAEFLILVPLYFIFFRKREGLGRGSFRLDVFFTLFSLIMIIQLFVPYLL
                     AVRKTEEWMISQMSFSGAILWINIF"
     gene            complement(481..939)
                     /locus_tag="ACHJ12_002070"
     CDS             complement(481..939)
                     /locus_tag="ACHJ12_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989856.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILLR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIVLLVCIHSLITTIK"
     gene            1397..1906
                     /locus_tag="ACHJ12_002071"
     CDS             1397..1906
                     /locus_tag="ACHJ12_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989857.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
     gene            1995..2618
                     /locus_tag="ACHJ12_002072"
     CDS             1995..2618
                     /locus_tag="ACHJ12_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4989858.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            2707..2940
                     /locus_tag="ACHJ12_002073"
     CDS             2707..2940
                     /locus_tag="ACHJ12_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989859.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(2960..3169)
                     /locus_tag="ACHJ12_002074"
     CDS             complement(2960..3169)
                     /locus_tag="ACHJ12_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4989860.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            3668..4165
                     /gene="tssB"
                     /locus_tag="ACHJ12_002075"
     CDS             3668..4165
                     /gene="tssB"
                     /locus_tag="ACHJ12_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4989861.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            4186..5730
                     /gene="tssC"
                     /locus_tag="ACHJ12_002076"
     CDS             4186..5730
                     /gene="tssC"
                     /locus_tag="ACHJ12_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4989862.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            5746..7083
                     /gene="tssK"
                     /locus_tag="ACHJ12_002077"
     CDS             5746..7083
                     /gene="tssK"
                     /locus_tag="ACHJ12_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4989863.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            7080..7733
                     /gene="tssL"
                     /locus_tag="ACHJ12_002078"
     CDS             7080..7733
                     /gene="tssL"
                     /locus_tag="ACHJ12_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO4989864.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            7736..9436
                     /locus_tag="ACHJ12_002079"
     CDS             7736..9436
                     /locus_tag="ACHJ12_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4989865.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENVGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP
                     "
     gene            9471..9962
                     /gene="hcp"
                     /locus_tag="ACHJ12_002080"
     CDS             9471..9962
                     /gene="hcp"
                     /locus_tag="ACHJ12_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFO4989866.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            10132..12819
                     /gene="tssH"
                     /locus_tag="ACHJ12_002081"
                     /gene_synonym="clpV"
     CDS             10132..12819
                     /gene="tssH"
                     /locus_tag="ACHJ12_002081"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4989867.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRIIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWHDEDGIVIAFDGINSDYPQESIYGQC"
     gene            12806..13447
                     /locus_tag="ACHJ12_002082"
     CDS             12806..13447
                     /locus_tag="ACHJ12_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989868.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            13444..13671
                     /locus_tag="ACHJ12_002083"
     CDS             13444..13671
                     /locus_tag="ACHJ12_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989869.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            13750..16269
                     /locus_tag="ACHJ12_002084"
     CDS             13750..16269
                     /locus_tag="ACHJ12_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO4989870.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            16266..17948
                     /gene="tle1"
                     /locus_tag="ACHJ12_002085"
     CDS             16266..17948
                     /gene="tle1"
                     /locus_tag="ACHJ12_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFO4989871.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            17968..18645
                     /gene="tli1"
                     /locus_tag="ACHJ12_002086"
     CDS             17968..18645
                     /gene="tli1"
                     /locus_tag="ACHJ12_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFO4989872.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVMPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            18676..18942
                     /locus_tag="ACHJ12_002087"
     CDS             18676..18942
                     /locus_tag="ACHJ12_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO4989873.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            18946..20094
                     /locus_tag="ACHJ12_002088"
     CDS             18946..20094
                     /locus_tag="ACHJ12_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO4989874.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPVVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            20087..23476
                     /locus_tag="ACHJ12_002089"
     CDS             20087..23476
                     /locus_tag="ACHJ12_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO4989875.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTSPRYVTVQDI
                     RHTLRRQHGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAEFSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLTQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAASGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
     gene            23476..25074
                     /gene="tssA"
                     /locus_tag="ACHJ12_002090"
     CDS             23476..25074
                     /gene="tssA"
                     /locus_tag="ACHJ12_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO4989876.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            25208..26971
                     /gene="tssF"
                     /locus_tag="ACHJ12_002091"
     CDS             25208..26971
                     /gene="tssF"
                     /locus_tag="ACHJ12_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4989877.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVCNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            26926..28026
                     /gene="tssG"
                     /locus_tag="ACHJ12_002092"
     CDS             26926..28026
                     /gene="tssG"
                     /locus_tag="ACHJ12_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4989878.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTTEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            28007..28543
                     /gene="tssJ"
                     /locus_tag="ACHJ12_002093"
     CDS             28007..28543
                     /gene="tssJ"
                     /locus_tag="ACHJ12_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4989879.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            28546..28977
                     /gene="tssE"
                     /locus_tag="ACHJ12_002094"
     CDS             28546..28977
                     /gene="tssE"
                     /locus_tag="ACHJ12_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4989880.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            29235..30239
                     /locus_tag="ACHJ12_002095"
     CDS             29235..30239
                     /locus_tag="ACHJ12_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO4989881.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            30586..30888
                     /locus_tag="ACHJ12_002096"
     CDS             30586..30888
                     /locus_tag="ACHJ12_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989882.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPARRQVKSPVATKP
                     VQKKKSAAVRKVSQPLVAEKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            31085..32286
                     /locus_tag="ACHJ12_002097"
                     /pseudo
     CDS             31085..32286
                     /locus_tag="ACHJ12_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            32521..34266
                     /locus_tag="ACHJ12_002098"
     CDS             32521..34266
                     /locus_tag="ACHJ12_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO4989883.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            34332..34637
                     /locus_tag="ACHJ12_002099"
     CDS             34332..34637
                     /locus_tag="ACHJ12_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989884.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            34692..35006
                     /locus_tag="ACHJ12_002100"
                     /pseudo
     CDS             34692..35006
                     /locus_tag="ACHJ12_002100"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            35331..35480
                     /locus_tag="ACHJ12_002101"
                     /pseudo
     CDS             35331..35480
                     /locus_tag="ACHJ12_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35655..36089
                     /locus_tag="ACHJ12_002102"
                     /pseudo
     CDS             35655..36089
                     /locus_tag="ACHJ12_002102"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            36232..36321
                     /locus_tag="ACHJ12_002103"
                     /pseudo
     CDS             36232..36321
                     /locus_tag="ACHJ12_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36726..38447
                     /locus_tag="ACHJ12_002104"
     CDS             36726..38447
                     /locus_tag="ACHJ12_002104"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4989885.1"
                     /translation="MAKMIPAFGPIDNNSCGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFITFNNLLSE
                     YIRQQTPESLCDVFTWHKLCHQARNKLSLSPESPAGWFESGCCEDLLNALNKNKLRDY
                     DALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRIINPQFS
                     FNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLNEILQLK
                     ESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYSESMEDA
                     QLFCAYSRATTAFIAIYDSEKLAWREKINFCINWLTERRFREQLMKQSIILPPVFLWP
                     VFSAKES"
BASE COUNT         9535 a         8622 c        10585 g         9835 t
ORIGIN      
        1 aaatatatta atccataaaa tggccccgga aaaagacatt tgcgagatca tccattcttc
       61 cgttttcctc actgccagca gataagggac aaataactga ataatcatta ttaaagagaa
      121 aagagtaaaa aaaacatcca gtcgaaaaga acctcttcct aatccttctc tttttcggaa
      181 gaaaataaaa tacagtggaa caagaattag aaattcagca agaaataatg ctggaacaag
      241 aagtcccctg gatataagtt cctgcctgtt tgtaaggaat gcaggaataa aggtaataga
      301 aaatgataag gtaaacattg aaaagcagcc gactgatgac tgttttttta tagttgcatt
      361 tttatttttt gttatttcca tttatattcc cttacttgtc gtttaaaatt cttctatata
      421 acaggaagag gtaaccatag aatgaacgtt tattttcctg tatcagaaca gccataactt
      481 ttattttata gttgttatca gtgaatgaat gcaaactaat agaacaatga gaaaaataat
      541 ggagaaaaag cgacggaccg gacgccatag ttcatattct cgtgtatcag cctcacgtat
      601 caacctttct gaaatggttt cggtttcttt tcctcgcagc agaatatgtc tgtaatattt
      661 cctcatgaca tataccgaaa actgaaacca gattaatatg gatggacaca caacacataa
      721 acttctgatg atatcacccg ggaaagtatc ttgggtgggt aaaagaaagt catatactct
      781 ggataaaatc atcatcgaga taaatgagac gccagcacct atccagtatt cgatatgaga
      841 agatgctgca ttatatgcgt cagtaaaatt tttctttctt aatgaaggtg gaagtgctga
      901 aagttcagga atgcgttttc ttgtccagta tattttcata tagtaactca tataaaaagt
      961 gttctacttt actatgatga tgacgattta taaaaggctt ataatgacta cttctccttt
     1021 cattctcgaa tttcacgata atgatagaga taatcattat caattgatag tgtcagtaat
     1081 tttgacttac atcacacttt catggtttaa tcaggtgatt gatttaatga aaagagtgtg
     1141 tttgtggatg tattgtgtat atgtcttacc gggcatctgt tcttacatta attaatatat
     1201 tgctatttaa gtattggtag tattgtcagt aacattcttt tggttaattt agttattatt
     1261 cctcactgat taattgtcct tccgttctta ttgtttgtaa cttgtgtttg tatcgtacct
     1321 ttcaggtgtt gatgcgcgct ttactgtgtc tgcaccggct tgacaaaatt caacaggggt
     1381 tgaaaaggaa catttcgtgc aaataaccga agccttaatt tcagagccgg gggaaatccg
     1441 gcgttttgtt cagcaggcgg tggaccactg gccaaatctg ttggcatttc acttcacgct
     1501 ctattcggca gaaggaatca atggtcaaca gattcagaca ttttgtagtt cctttcaccg
     1561 acgggtacac gaacgtatta cggagcataa tcacacggtc agcccatcag cgccggtggt
     1621 attacgctgg ttgcgggaac aacatgaagg agcacagata cgatgtctgt tgctgctcag
     1681 ccagacgagt atttgccatc cgcgagtcgg cgtcatggct gatgaagaat gtgcgcaact
     1741 ggtggattta ctgcaacaga cctggccggt gataagtgct ggcggacaat gccgggtgga
     1801 aagatgtttt cgggttgccc ggcctggttc atcagggcaa tacgtggcgt taaaaacggc
     1861 tgttcagtct ctgatgccac ggggtattgc aacaatcatc cggtgatgga cgggagaaat
     1921 atcgactctt ccgttagttt ttacagactg ataaagtttt gccagctgaa tctttatacg
     1981 gaatactctt cagtatgcgt acacgaattg actatcttgc ggataaatac agttttactg
     2041 aactgaatga acttccacgc cttcaccagc agtggcagga tgttctggag gagtgccggc
     2101 agacagaggc cgggccggaa gaacggctgc gtattgcact gctgaatgtg gattacgtca
     2161 ccagttttga actgcctttt cgcttgttgc ttacccggac accacaactg attgccgcgc
     2221 tgcgtgaaga atggggcatc agccagaaaa atgtggtgtt caatgataaa cgatttggct
     2281 gcgtgtacag cctgaaggcc agcctttccg gtgtaccgga tacataccgg tatcatctgt
     2341 ctcatcgtat tcgccgggtg attgggaatg aaaacacatc atcgccatac cagcagattg
     2401 cccgggaagt gaaagcgccc cgtgaacggc tgaagcatgc gctggaggcg gggttgctgg
     2461 tgactgcact ggacggcctg ttctggttcg gtagtcagcg tattgcggct gatgtcctga
     2521 ggctgagaaa gagcggaatg ccggtggtga caacgaccgc ggaggtgcat gataatctga
     2581 cgggaacaac ccgcaaagta ccggcctacc atctctgatt cgaccatcag ggggaatatt
     2641 gctctggcag cagcagtacg acattcttta tccccgagcc tgaaaacatg tgctaaggaa
     2701 acgggaatgg caaggtccga ttacgatatt atcaatctgt ctccggaaca tgaactgaat
     2761 gagtggctgg cagagagagg ttatgccgga caggcggata accgtaaccg ggtggcagaa
     2821 attgttaccc gcaaattgca ggacagcttt tatatcaacg tttcttggga tgcgctgaac
     2881 acggcataca gtgaacaccc ggagtggttt tctgggcttg tctccgggga tgagaattaa
     2941 taagtggatt atgctgccat caggcagtgt gattgccggc agggggttct ttccggggtt
     3001 cgggtttgaa ttttcccagg aagttgtaca tcggccgcat gcagggatgc ggatagccgt
     3061 cccgcataaa caccacccgg gtttcctcaa cactggtcac ggtgacaagc gcgccatgat
     3121 cgtctctgta tcgctgattg atttttggta atggaatatc ttctgacata gacttcagaa
     3181 cggtaaccgt tcactaaatt ttcatgtggt atcactgaat caggagacga cgtttcctga
     3241 tgtgcgttgt taattttctg tttttaaaaa atattttctc tctcttcttt attttccgtg
     3301 tcttttattg ctctcctctt tcatgtcctt ctctttttct gatatgccct taaatgctgc
     3361 cgaatcatca ctcctcgtga atgtcagtgc tggtaatcat tcggtataac catctgtatc
     3421 aaaagcacta accggaataa ttttctgcat ttttgtatta cccctggata taagttttga
     3481 taaatgctga ttttgcgata tgtgtcagaa taaagatctg gttattggca ttaaaacgat
     3541 cgatatgtct gtcatattga gaatttgcag atcttcgtcc tataatgggt gaaattaaat
     3601 cagtgcacaa ggggaggcct tctgtggcga tgggctccct gggatgcagg tttcacagga
     3661 gaacgtcatg agcagttcgt ttcagaatga gatcccgaag gcaagggtga atctgaaatt
     3721 agacctacat acaggtggtg ggcagaagaa agtggagctt ccgctcaaac tgctggttgc
     3781 cggtgatttc agtaacggac aggaagagcg cccgctgtca gagcgcataa aaatggatgt
     3841 taataaaaat aactttaatg ctgttctggc tgatatctcg ccgtcagtac gcctgaatgt
     3901 ggagaacaca cttgccggtg atggtactga agagagcatc agcctgactt tccgtaatat
     3961 gaacgatttc acgccggagc aggttgcccg acaaattccc cgtcttaaag ccatgctggc
     4021 catgcgcagc cttctgcggg atctgaaggc gaatctgctg gataacgcca ctttccgtaa
     4081 agaactggaa aaaattctgc gggatcccga actgagtcag gccttacgtg acgaactgag
     4141 tgcactggtg ccggaacagg tgtgattaac ggattaagag agagcatgct gatgtctgta
     4201 cagaaagaaa ataatgtggc gggaaatgcg gtttctgaaa cgcattccgg cgacagcgta
     4261 tatgcttccc tgtttgaaaa aattaatctg aatccggtat cagccctgag tgcgctggat
     4321 atctggcagg atccgcaggc catgtcagag gcatcagcag atgaacgtct gaccgcaggg
     4381 atgcaggtgt ttatggaatg cctggcgaaa gcgggagcac cggtggagaa actcgataag
     4441 gcgctgattg accatcatat tgcagagctt gattatcaga tcagccgtca gctggatgcc
     4501 gtgcttcatc atccggaatt tcagaaagtg gagtccctgt ggcggggcgt gaagtctctg
     4561 gtggataaaa cggacttccg ccgtaatgtg aaaattgaac tgctggatct gtccaaagac
     4621 gatctgcgtc aggattttga ggatgcgccg gaaattattc agagtggtct ttatctgcag
     4681 acttatgtgg cggagtacga tacgccgggt ggtgaaccca ttgcagccct gatttctgcc
     4741 tgggagtttg atgcgtctgc gcaggatgtg gcgctgctga aaaatatttc ccgggtggct
     4801 gcgtccgcgc atatgccatt tattggttca gttggcccgg ctttcttccg gaaggatacg
     4861 atggaagagg tggccgccat taaggatatt ggcaatcact ttgagcgtgc cgagtacatc
     4921 aagtggaatg ctttccgtga aacggatgat gcccgctata ttggtctggt gatgccacgt
     4981 gttctgggac gcctgccata tggtcctgat accgtacctg tccgcagctt taactatgtt
     5041 gaagaggtga aaggtccgga tcatcataag tatctctgga caaatgcgtc ttttgctttt
     5101 gcggccaata tggtgcgcag ttttgtcacc aacggctggt gtgttcagat ccgcggtcct
     5161 caggccggcg gtgcggtgca ggatttgccg atccatctgt atgatctggg aaccggtaat
     5221 caggtcaaaa ttccgtcaga ggtgatgatt ccggaaactc gggagtttga attcgctaat
     5281 ctgggcttta ttccgctctc ttactacaag aaccgggatt atgcctgctt cttctccgct
     5341 aactccaccc agaaaccggc actgtacgat acgccggatg caacggcgaa cagccgtatt
     5401 aatgcccgtc ttccctatat cttcctgctg tcccgcattg cgcattacct gaagatcatt
     5461 cagagggaga atatcggtac caccaaagat cgccgtctgc ttgaactgga actgaataac
     5521 tggatccggg gtctggtgac ggagatgacc gatccgggtg atgaactgca ggcctcacat
     5581 ccgctgcgtg acggaaaagt ggtggtggaa gacatcgaag acaacccggg atttttccgc
     5641 gtgaaactct atgctgtacc acatttccag gtggaaggta tggacgtcag tctgtcactg
     5701 gtttcgcaga tgccgaaggc aaaagcgtaa ggcaggcgtc gggaaatgaa gatttatcgt
     5761 ccactgtggg aagacggggc ttttctgatg ccccagcagt ttcagcagca ggctgcctgg
     5821 gatgttcatc tggcagacag cgttgcccga atggggctgg cgcatccctg gggcgtggtg
     5881 gctgcagagt ttgatgactc tctgttaccg ctttcacggt tgaatgccac ccgtctgatt
     5941 gttcgtttcc cggacggaac gcttattgat acggaacgtg cggataatct gcctccggtc
     6001 tgtgatttat cgacggtttc tgagcgttcc ctggtggata ttgtgctggc gctaccgtta
     6061 ctgaatgcta acggtggcaa ccttgatgat ggcagtgaga gcgagcgtcc gcgtcgctgg
     6121 aaatcagaaa gggtgaatgt gcaggagctg gccgggcatg agcaaagcga agtggcggta
     6181 ttgcggcata acctgactct gcgcatggct catcaggaaa atgctgcctg gctgacctgt
     6241 cctgttaccc gtcttgttcg agatgcacag ggacagtggt gcagggaccc gcgttttatt
     6301 ccccctctgc tgacgctgtc tgccagtcct tcgctgatga ctgagcttgc ggagttgctt
     6361 catcacctgc aggcgcgtcg ccagcgactg atgtccatgc gtcgggagaa caatgcccgt
     6421 cttgcggatt ttgcagtggc ggacgtgtcg ttgttctggt tgctgaatgc cctgaacagt
     6481 gcggagcctg ttctgaaaga gttgcttgac atgccttacc gccacccgga actgttgtac
     6541 agggagcttg cccggctggc aggctccctg ctcacattct cgcttgagca taatgtggat
     6601 gcggttccgg cataccgtca tgaaacacct gaaaatgtgt ttccaccctt gctttctttg
     6661 ctgaaccagc tgctggaggc aagcctgcct tcccgtgtgg ttttcattga actgaaacaa
     6721 aatggtgtga tgtgggaggg ggcattacat gatgcgcgtt tgcgcgaagg ggcggatttc
     6781 tggctttcag tgcgctcttc catacccggg catgaactgc agacgaagtt tccgcagcta
     6841 tgcaaagccg gaagccccga tgatgtgtct gaggtggtga atgtggcgct gagcggggtc
     6901 attatccgtc cggttacgca tgtaccggcg gccattccgc tccggctgga aaaccagtat
     6961 ttcgcgctgg atttaagtac ggatgcagcc cgggcgatgc tggacgcggg gcgttgtact
     7021 ttctacaccc cggcatcgct gggggatgtg aaactggaac tttttgcggt actgcgtaca
     7081 tgaataaacc tgttatctcc cgggctgaac agatttttta tcccggctgg ctgatggtca
     7141 gccagctgcg ctgcggacaa ccggttgagg atggtaaggc cctgtatcgc cgtgcctgtc
     7201 agctggtgac gcaggcgcgt gaagagctgg ctgaagcaga attcagtcag gaaagcagtg
     7261 aaatcatgtt gtatgccttc tgcgccctgc tggacgagag tgtgctgaac cgtgaaaaaa
     7321 cagacgatgg ctggcacacc tggcagcagg acccgctgca ggctcatttt tttggtacgc
     7381 tcaatgccgg agaagagctc tgggagcgta ttcgtgagca actgaaatta cctgcgccgg
     7441 atgtggcggt actgacatgc ctctgccgta cgcttcagct cggttttacc ggtcagtacc
     7501 ggtcgcagga tgatgagcgt cgcgaagatg taattcgcgc actgacagcg cgtgttcctg
     7561 catttacttt cgcacaggat gcgccggtgg tggtgcgtgc accgggttac cgtgctggca
     7621 gaacgatgta ctggttgtca tgggtggcgg gtattgtgac gctggctggt ctctggtgtg
     7681 tgctttcttc tgtgctcgca gaccaggtgg cacggcttac cgggcaggga taaccatgca
     7741 cgtacgttcc cgtctctggc tgacaatact gagtctgttc ctgacactgt ggctgatttc
     7801 gggattcctg ccactgtctg cagtaaacca gataatactg accctggttt cagttgtggc
     7861 ggcggggctt gtgctgtggt atcagtggcg ttgtctgtct tcccgtcgtg atatcccgga
     7921 gaatgtcggc gatgacggtc tgccgcctga gtattttcag gggcatgtgt tgcttgttgc
     7981 cggaaactca gatgcgtggt tcgaacccgg tcaggcatac cgggaaacag catcgggatg
     8041 gtatcttcgt gtggatacgc cggagcagat gagaatactg gcagagcggt tgtcgtacag
     8101 ccgtccggcc ctgtgtggcc aggtcgtggt catgctggga cttttaccgg agcagcatac
     8161 ctcagatgaa gctttcctgt cgtggttgcg gaactggcag cgtgcagtca tgcagtgccg
     8221 gcagtggctg aataccatgc cgccggtgct ggtgacattg atggtttcag ggccgtcacc
     8281 acaaaacaca tccttaaata agcactctgc acactggttt acactgacac cggacctgtc
     8341 gggaatgcac atacgcctga cggaagccgg agccattccg gcagaggaat ggctccggga
     8401 gtgtgatgca gcgtccaggc tccggtgcgt cagtgaactg ttatggctga atgccatgtt
     8461 gtcctggtac aaccgggtta ctgatgccct gacagatatt tgccggcagg agcactttta
     8521 tttccggccg gttgccgttg gattctgtct gacgtcggtt gcggcccggc ccggtaatct
     8581 ctggcaacaa caactgacgt cactcactgt actgccaccg cctgccggcg tgtcatcaca
     8641 ggcactgccg ttgccggata ttctgctgtc cggtattccc cgtcatcgtg gactcagtca
     8701 ctggcacaga ctttgtctcc agacgggggc gattgtcggc gtttttcttc tgctggctat
     8761 gctggcgtct tttatgaata accagcgact ggttcgcagc gtgggggaac acctgacggc
     8821 ctggcaccgg atgcgcggat attcatctga atcgaaaaaa actgcactgc aacgtcttca
     8881 ggctgacagc cagttactga acgactggca gcaacgtggt gtgccgcagc gttacggact
     8941 gggattgtat cagggaatgt ggctgatacc gccggttgag cgagcgactg gtgagtggat
     9001 tgcaccactg cctccccgtc cggtaataca gaaagttact cagacgccga aaatggttcg
     9061 ccttaacagc ctggcgctgt ttgatgccgg taaatggacg ctgaaaccgg gcgccacaaa
     9121 atggctggtg aatgccctgg tggatattaa ggcaaaagcg ggctggctga ttgtggtcag
     9181 tggtcacacg gacaatacgg gcgacccgca gcgcaaccag gccctgtcac tgaagcgcgc
     9241 agaggccgtt cgcgactgga tgcgggatac gggcgatatc cctcaaagct gttttgccgt
     9301 acagggatat ggtgagagtc gtcccgtggc tccgaatgag acggcggaag ggcgagcgcg
     9361 taaccgccgt gttgaaatca gtctggtacc gcaggccgat gcctgtcgtc tgccggacat
     9421 cacccgggcg ccgtaacagg gcgcgtaaga caacgagagg gagtaacttt atggcaattc
     9481 ctgtttatct gtggctgcag gatgatggcg gagcggatat taaaggctcc gtggatgtga
     9541 aggaccgtga gggcagtatt gaagtggtgg ctcaggagca ctgcctttac attccgacgg
     9601 ataacaatac cggtaagctg accggtaccc gcattcacac gccgttcctg tttaccaagg
     9661 aaattgattc ctccagcccc tacctttaca aggcggtgac cacggggcag accctgaagt
     9721 ctgctgaatt taagtggtac agaatcaatg atgccggtca ggaagtggag tatttcaata
     9781 cgaagcttga gaacgtgaag gtggtgaagg ttaacccggt catgcacgac atcaagaatc
     9841 ccgcctacga gaagcataac caccttgagc aggtggagct gcgctacgag aaaatcacct
     9901 ggacttataa ggacggtaac atcattcatt ctgacgcgtg gaatgaacgt accactgcgt
     9961 aattctcccg gacgggcagg gtatcctgtc cgtcattttt ctttttttct tcagtatctg
    10021 ttggcgggtg ctgaagaaag aattctcaat cctgaacaca gacctgatgc gcccgggtta
    10081 tggcgaaggg cggtagcgtg tctgaaggtc ttctgacagt gagtgaataa catggaaaat
    10141 ccggcagccc tgttacgtcg tcttaatcat tactgtgccc gtgcactgga aggcgcagcc
    10201 tccctttgcc agacccgggc ccatgcagaa atcaccccgg agcactggtt actgaaactg
    10261 ctggagcagg gggaaggaga ccttaccgtg ctgggccggc gttatgactg ggatatggac
    10321 gcgatatggc agtcgctgct cggctggctg gataaccagc cccgtagcgt acgcagccgc
    10381 ccgcagcttg cgccgtccct gaatgccctg ctgaaacagg cctggatggt ggcctcatta
    10441 cagggagaag aacatatccg cagcgtgcat ctgctgggtg ccctgacgga aaatccgcac
    10501 ctggttcgct gtgacggggt gtggcccctg ctgacactga gtcagagcca gttgcagcgt
    10561 ctgtccccgc tgctggatgc gcagtctgat gagtgtccgg agagattaca ggatgcagag
    10621 cctgtgctgc ctcagggaga caatgtgacc tttatcgggc gtccagtcgg tgcggatacg
    10681 gcgggtatac cgtcaggtga cctgccgcct gtgttacaga gtgcgctgga taaattcacc
    10741 cgggacatca cggccagcgc gagagagggg aaaattgacc cggtatcggg acgcgatacg
    10801 gaaatccgtc agatggtgga tattctctcc cgccgccgca agaacaaccc gattctggtg
    10861 ggggaccctg gtgtggggaa aacggctctg gtggaagggc tcgccctgcg tattgcggaa
    10921 ggtaacgtac cggaatcact cagacccgtc accctgcgca cccttgacct cggcctgctg
    10981 caggccggtg ccggcgtgaa gggtgaattt gaacagcgcc tgaaaaacgt gattgatgcc
    11041 gtgcagcagt caccggctcc tgtactgttg tttatagatg aagcccatac ccttatcggg
    11101 gccggtaatc aggccggcgg cgcggatgcg gccaacctgc tgaagcctgc gctggcgcgg
    11161 ggtgaactgc gtaccattgc cgccaccacc tggtctgagt acaaacaata cctggaacgt
    11221 gacgcggctc tggagcggcg ttttcagatg gtcagagtgg acgagccgga tgatgagacg
    11281 gcctgtctga tgctgcgctc cctgaaatcc cgttatgcgg aacatcataa cgtgcatatc
    11341 accgatgagg cggtacgtgc tgccgtcacg ctgtcgcgcc gttatctgac agaacgccag
    11401 ttaccggaca aggccgttga cctgctcgat actgctgctg cccgtgtgcg tatgagcctc
    11461 gacacggtgc cggaacagct gacccgtatc cgttcacagc tcgcttccct cggtatggaa
    11521 aaacaggcac tgctggaaga tattgccgtt ggtcatcaga atcacggcga acgtctgtct
    11581 gccattgagc aggaagagaa tgtgctgatg acagcacgtg atgacctgga gcagcagtac
    11641 gcccgggagt gcgaactcac cggtgagtta cttgaaagtc gcagcgatat ttcccgtcag
    11701 agtgagacac tgcatctgca gcaggcactg cacgacattc agcagaatca gcccctgctc
    11761 agtgtggatg ttgacgtacg caccgttgcc ggtgtggtcg cggactggac cggggtgccg
    11821 ttatcttccc tgatgaagga cgaacagacg gaactgctgc atctggaaaa ggatatcggc
    11881 agacgggtgg tcggacagga cgtggcactg ggatccattg cgcagcgtct gcgtgcggcg
    11941 aaaacgggtc tgacatccgg taacggcccc cagggcgtgt tcctgctggt cggtcccagt
    12001 ggtgtgggaa aaaccgaaac ggcactggcg ctggcagatg tgatgtacgg cggcgaaaaa
    12061 tcactgatta ccatcaatct gtcggaatac caggaacccc atacggtttc ccagcttaag
    12121 ggttcaccgc ccgggtacgt gggatacggt caggggggta ttctgacgga agctgtacgt
    12181 aagcgtccgt acagtgtggt gctgctggat gaagtggaaa aggcccaccg ggatgtgctg
    12241 aatctgttct accaggtgtt tgaccggggc tttatgcgcg acggtgaggg gcgtgaaatt
    12301 gacttccgta atacggtcat tctgatgacc tccaatctgg gcagtgacct tctgatgcag
    12361 caactgagcg aaaagccgga gacaacggaa tcggagctgc atgagcttat ccgtccactg
    12421 ctgcgcgacc acttccagcc agcactgctt gcccgtttcc ggaccgtgat ttaccgtccg
    12481 ctgacgcagt ctgccatgcg catcattgtg gagatgaagc ttgcacaggt ctgtgaacgc
    12541 ctgcactgcc attatgggct gagcacatcg gttgatgaac gtgtgtacga tgccctgaca
    12601 tccgcctgcc tgctgccgga tacgggcgcc cggaatgtgg agagtctgct gaaccagcag
    12661 ctgctgccgg tactgagccg gcagttgctc agctatcttg ctgccagaca gcatccgacg
    12721 tacctgtctc tgacgtggca tgatgaggat ggtattgtga tagcgtttga tggaatcaat
    12781 agcgattatc cgcaggaaag catatatgga cagtgctaaa gaaattgccc gggatatgct
    12841 ggaggccgta aatattaatg gggatgggtt tctgggggcc gtcgggaagg gacttatatc
    12901 tttgccagtc agtctcggtt atatgggata tgattttata gataccgggc ataggcggga
    12961 aaaccagaat gacaaattca gaatggccag actggtagaa aaaggcattt tcagccgtga
    13021 tattattggg aaaattatca gtatttgtct tgaagacttt gtatcccgga tcaatatgga
    13081 aaaaacaggc tccattgtga agaacgtttc aggagatctg gcaggtagaa tggcatttgc
    13141 tgaattgacc ggggttaaac tgggagaggt tctggtatcc agagggctgg ctgctttttt
    13201 tgcagggagt tttgccgggt tgttattatc tgttggcgca gagacatcgc gtgcgattta
    13261 cacatcccgt tttctgaaag acagaaatcc ggtgttgcat gataagctga aagctcaggg
    13321 ggatttggat cttttgtact ttcttgttga agatattgtg cgcccgtttg aacgagcctg
    13381 tattgtcgca gaccgaaatt cggctgagtt tgaccggata tgcgaatatt ttttcggtgg
    13441 tttatgaaga taaaaagaac attacttata atgctttccc gatttatccg tggcgcaggt
    13501 atggggttag gtgcgtcggg tattgtattt acagtctggt tcttttttct ctcatctgct
    13561 gaatttaaat atctgtgggg agggctctct gttgcgttat tttttgtcgg ttatctgatg
    13621 tatcgatttg catatgcata tatttacgat gaatgggatg attatcagta attctctctg
    13681 aaaataaagc ttaacggcca tcattatata attgcagttc aataccggta cagacatgga
    13741 gttaacaata tgagtctgac agatacactt tcaaaaacta tctctgcttc cctgaatcgt
    13801 taccatttaa ttattccctc ctgcccgtca ccactggatg ttgagcattt cagcggcagg
    13861 gaggagatga gtcagaccta ttattacatc atcaatttta ccagtactga caacgacctg
    13921 gatgccgggc tgctgctgcg taaatcagcc acatttacaa tgggtacggg agggcttaag
    13981 gagcaggtga gccagaaagt agtacatggt gtcattacag attttcgtcg tatatcgggc
    14041 tccgccgacc aggcaaaata ccaggtgata ctggaaccat ttattaaact actggacagg
    14101 cagcatcgtt ctcaccgatt tttcgttaac aaatcggtgc cggaggtggt gacagaggtt
    14161 ctccaggagc acggcctgaa aggctgggag tttgagttcc gtctcaaaaa aacgtatccg
    14221 aagcgtgaac aaattaacca gtatcaggaa agtgacctgc aatttattca gcgtttgctg
    14281 gctgaagtgg ggatttttta ttttttcacc ctgcaaccgg acacgcagac ggaaatagtg
    14341 cattttggtg acagccaggc tgcgctgacg tttgataaga cgctggcgat aaacagtccg
    14401 tccggaatga atgataacag agcggattca gtgtgggggc tgaatgtgac gcacaatgtg
    14461 gcagaggcca gtgtcaccac aaaggcttat aaccatcgtg aagcacaata tctgttgcag
    14521 tccgccccgg cagacatgac gcatggtgat ggtgacggaa ttaactatgg tgaggtgtac
    14581 cactaccatt tccgtcatct ggaacggggg gataaaatcg acccggtgcc ggagacggcc
    14641 aatttttatg cccgccttgg tcatgaacgt tatctggcgg agcaggtacg gataaccggc
    14701 aacagcacag acgccacgct ggcgcctgct caggttctga ctatcaccga cagtcttccg
    14761 ccgacgttgc ctgcgctgct gcgcaatccg gtgttgctta cctgtgtcgg tttttctgca
    14821 agtcgcaagg atgccctgca ggttgtactg aagggcgttc cttacagtga agttatctgc
    14881 tggcgaccac cgttacttcc tcgtccgaaa atcaccggta ccatgacggc ccgtgtgacc
    14941 agtgcaaaag aaggggatat ctacgcctgg caggatgcat caggcatgta ccgggtgaaa
    15001 tttgatgctg accgtgatga taaaaatccg ggacaggaaa gcatgccggt acgtctggcc
    15061 aaaccctaca gtggtgatgc ttatggtttt cattttccgc tgattcaggg aacggaagtg
    15121 gccattgcct ttgaggaagg tgatccggat cgcccctata tcgcgcatgc cctgcatgat
    15181 tcacgtcatg ttgatcacgt taccgataag aacggtacgc gcaatgttat ccgtacgccg
    15241 gcaaataaca agttgcggat ggaggacaaa cgcggtgagg agcatatcaa gatcagtacc
    15301 gagtttggcg gtaagacaca acttaatctt ggtcataacg ttaatgcctc ccggaaactc
    15361 aggggagaag gcgctgagct gaggactgat gactggataa gtatccgggg aggaaaagga
    15421 atatttatca gtgcagatat gcagcctcag gcacaaggaa agatgctgga tatgaatgaa
    15481 gctatccgcc aactggagca ggcattatct ctggcccgaa gtatggcgaa ggccgcaact
    15541 gctgcaaatg ctactcaggg agatataagt tgtcagcaac ggttgaattc atcgctaaca
    15601 gatctcactg cgccgggaat gctgttgcat gctcctgatg ggattggcat ggttagtgca
    15661 cgggcgttac gaattgcttc gggtagtgaa agcgtgggca taatgtcggg agataacact
    15721 gatattagtg ccgggcagtc atttacagtt gtagcagaag gtgctgttag cctgttgagc
    15781 agaaaccagg ggatgcaact gttagcagca aaagggcggg taaatattca ggctcaaagt
    15841 gacgatttgt caatgagttc ccaacagaat cttgatattc aaagttctga gggcaaagtg
    15901 actgtttctg ctaatcagga actgatactg gcatgtggtg gagcttatat caaactaagc
    15961 ggtggaaata ttgagctggg atgcccggga cagatcctgt taaaaagcac gaatatgcag
    16021 aagatgggac caacaagtct tgatattgcg tctgttgaaa tgccgcgagg gtttggtggc
    16081 ggttttattc ttaccgatga agcgggagtg ccgcaacctt cgacgcctta tcgattaaca
    16141 acggctgaag gtgatattct acaagggatc accgatgaaa atggtaaaac agcgccagtc
    16201 aacacctcca ttccgtcagt agtaaaagtt gagtttggga aggtaaaaat tcatggagaa
    16261 acagaatgac aaaatatcag ggatatgacg taacagatgc cacccataaa accagtattc
    16321 ataatgactg gaaagttgtt gttgcaaaga aaaaaccggc gcggggtgtg actttaacca
    16381 ttggtatatt tttcgatggt accggaaaca atcgggagaa tacagcatct cgcctgatga
    16441 aatttaatga gtgtagtgca gccagacagg gggtaaatca aaaagatgca caaagctgtg
    16501 aggactttct tgaagaaatt aacaagaaca gtatctcgaa tggcagttat cgaggatatt
    16561 attccaatat tcactggcta aattcacttt acgtagcaga tcagactttg ttggaaagtc
    16621 aggttgaagc acaaattcag acttacattt ccgggatagg gactgcggct ggtgaagcag
    16681 attcagtcat tggcatggga ttaggcacca gtattctgga tatatttgaa ggtgtggtga
    16741 cgaaaaccga cgaggcaatg gaacgaatca cccaggcgtt atcagaattt atggggttca
    16801 atttaaatcc tgatttttgt atcgcaaaaa tccagtttga tgtttttggt tttagccggg
    16861 gagcagcggc ggcaaggcac tttgccaatc gggttatgga acaggatcct gctatagcca
    16921 gagctattgc taaagggctc agaggcgatt tttatgatgg taaaccttct ggtgaagtcc
    16981 gttttctggg gctttttgat actgtcgcag ctattggtgg aatcagtaat ttcttcgata
    17041 ttaatggacg tagcaacccg ggagtaaagc tggaattgcg tccttcagtc gtgaagaaag
    17101 ttttccagat tactgcaatg aatgaatacc gatataattt cagcctgaac agtattaaag
    17161 gtatgtggcc cgaactggct ttaccagggg cgcattcaga tattggaggt ggttacaatc
    17221 cggtaggttc acctctgcag gaaaatgaaa gtctgtttct gagttgcccg gaatttgaaa
    17281 ttgtcagtga cgataccaga gaaacggata cgcgagtata ccgtaatgca gagcaagcca
    17341 gaaaaatgtt gatgactctg ccagccttaa aacatatttt gcctcatgga aaactaacca
    17401 ccaaaattcg ttccgtaggt gtgaacaaca gcaatcaacg tcgggctggt gtgatacaaa
    17461 agcaggtggg agccgcagta ttttttgagc gtatggcagt acctaatgac tgggctaatg
    17521 tttgcctgcg tgtgatgctg gatgctgctc aggaagcagg ggtattgttt gaaccaattc
    17581 gacaaacaaa tactgagtta caacttccat ctgaactaat atttcttgct gacaaagcca
    17641 ttgctcaggg aaaagcggta cgtttggggc aagagcctca ggcttttact gaggaagagt
    17701 tgtatattat cggaaaatac acccattgtt ctgccaactg gaatattgag tctgatggta
    17761 atttatgggt ggatcctaca acaggagaga tttttatcca tcgctttggt cctaaaggta
    17821 ataaggcgtt cgtttttccg aataaaccaa atgatcgctg gattcgttct gtatggtaca
    17881 tggatgatca gcagcgattg aatgataacg cagtaaaaaa tactaaagtt atgatgtctg
    17941 gtgtttaaca ccacagggtg gcaatatatg accagagata aaatcatttt tatcgcacca
    18001 ttactggcat tgttaactgg gtgtaattcg gttagctcac agtatgctga ttcggataag
    18061 actgaatggg gttataaaaa attaccctat gatgaatgga aatttctctt tatttatcca
    18121 aaggctctgc ctgcgctggc cactcaggca ttgatggtgg acggggaagg ttataagacg
    18181 acttatcagt atcttgatac cacaagaccg agtcaggtca gtgtagggcg ctggaatgac
    18241 catctgggcg cgactgagtc gtattataac aaggggaaag cattacctct gatgttacgt
    18301 ttttgctggg attcaataat tgataaaaag agttatgaaa ctctgatttt ttttaaaaaa
    18361 gggacctggg agcagatggt tatgccttac acagacaacc gctggaacga aacctattat
    18421 cgttcaacga tgattattgg tctggcacct gaagggaagg taagagtctg gctgggagat
    18481 cgcggtaatc cggtggtacc tcagttggat gctaaaatta ctacagtatc gggtgataaa
    18541 atgaatatgt gtaagggggt tactaaacat ccagatggat atgtttatta cggtgataca
    18601 ccagatttta tcaaagggaa aatttacccc tacggtaact ggtgactcaa agtaaaattt
    18661 atttttgaga gttttatgtc taaaggtttt gtattgcttg gtgataatac cacacatggt
    18721 ggtaaagtta tttcggcatc ttcaaccatg attgtcgatg gtaaaaaagt agctcttgtc
    18781 ggagatatgg ttagttgtcc tgtttccgga catggtacta atcctattgt ggagggctct
    18841 cctgaatgga tatctgatgg aagagctgtt gtgattgatg gatgtaaatg tctgtgtggg
    18901 tgtaaggtta tatccagtgc accggaatgt gcaataggat aacccatggg atggagcaga
    18961 acaaaatcat tatcaacaga attacctcat aaaccatcaa tgatgttgtg gtttatgaca
    19021 ggggcattta tggctgttgt tggtgctctg ttgtttatta tccgggcatc agaatatgtg
    19081 aaagcattaa atgatttcag tatctggtgg cttgcattga ctcctccggg gggctggttt
    19141 ttcctgtttt gcctgcgaca ctggcagtgg agcaaccaga tggatgaaca tttgttcttt
    19201 aaaaaagagg gggagtatgc tcagaaacag tgggaatcct gggccgaaag gtatcttgtt
    19261 attactgcca gttgtgttta tttgcctgat aaaataacgg tagctactct ttgtgatgaa
    19321 ttgcctctgc agtacggcct tgtaaagaaa atagattatc tttctgacag tgggcataag
    19381 gttgaagcct ctttacgtgt cttgcttagg gagataacgg ataaattttg tcagttacct
    19441 gtcgttctcc ctgtaaatgt gacgctgata accgatcagc ctgattctga aatcagatct
    19501 gcttttgttt ctgcatggga agtgttattt ccccaacgtg ttgttcctga caatatcgag
    19561 gttactcccg acttttccat ggggtgggtt gatgagcgtc tgaaacaacc agtgctgact
    19621 gtggatttga tactggtaat acagcttaat gggggaaatg catactctga cggactggct
    19681 gcacttttgt tgaccagcga tgatgttgcg caaaaatata acctgccgca tccagcccgt
    19741 ctgctacgcc cgatgtcgct ggatatcaac aagtttaatg atgaattcac acttttcctt
    19801 gaaacccaga cagctgcctg tcgtaccgct cgtgtactgg gggactgtta tcactgggaa
    19861 aagattgctg caccgctgat gactattgga aaccagtatg gtgccggatg ggaaccgtcc
    19921 ggacgtatgt tactggaaaa atggtgcggt atcccgggac ctgctgctcc gtggctgctt
    19981 actgctctgg cagccgatct ggtctgtctt ggtaatgatt ctctcctgac tttattctca
    20041 tccggagaag aacattttat cagtactgtt acatcaggaa accagaatga ataaactggc
    20101 ctgtctgtct ggtcgctttg gacgaccggg gattgtattt attggtgtgg ctgcgctctg
    20161 gtggctgata acccgatatg gagcatttct gggggcagaa acacgccggg atcaaatact
    20221 actgctgata ttgctgagtc tggggttgct ctttgtctgt tacctgcctg tgatgaaaaa
    20281 atatgttcag gaactgacat atcgacgtcg ggcccgtaag gagcaaaggt tacctgacga
    20341 tgaagagcga ctggctcaga cgtcaccgcg ctatgttact gttcaggata ttcgtcatac
    20401 tctgcggcgc cagcatggca gattctgggg acgtaaaata cgcattctgt tgataacagg
    20461 tactgcctct gaggttgagt tgctgacccc gggcctgaca gaacagttct ggcaggaaga
    20521 gcaggggaca ttgcttctct ggggaggaga cccctcgcag ccggaaaacg cagactggct
    20581 ggctgcgtta cgccgtctgc gttaccggcc tgctgatggc attgtctggg taacttccgg
    20641 actgagtgaa acattatccg caccactgac agaagatgcc cttgacaggg tttcccgtgc
    20701 ggtcagttca tgttgtgagc gccttggctg gcgtctgccg ctgtatgtct ggtcactgca
    20761 ggagagtccg gatgagcgag ggcgtattac tcagcccgtg ggatgccttc tgccagcaga
    20821 atttagttct gacaaactga aagcacaact acaggccatg ctccccgggc ttgttgctca
    20881 ggggatacag cagatatgct gtgcccctcg gtattatttt ttgttgtcgc tggcagagcg
    20941 attcagacga aatattgatg ctgtggtgga gccattatcc gtattactcc gtccataccg
    21001 tcagttgttg ctggcgggta ttgttttcag tccggcaact gtcggcggtg aacgaagtgt
    21061 cagacatcgc tggcgaatgg ataaccgctg ggaggcgttg ccggagacag tgcagcaatt
    21121 acctgtccgg ctacagcctt cccgcacagg tcataactgg cgcagaagtc tggccgttat
    21181 ggcggccata ctgatgatgg ctcagggaac aggaatggtg gtttcctttc tggcaaaccg
    21241 gagtctggtt gctgaagtac aggaacagat tcgtccggct cagaaccagc aactgtctcc
    21301 tgcagaacgt ctgcaggcgt tgctgaatct gcagaaatcc ctggcccgcc ttcagtaccg
    21361 ggaggagcac ggggcgccat ggtacctgcg ggccggtatg aatcagaatg cagacctgct
    21421 cgcggtcgta atgccccttt atgcacaaaa tgcacatctt ttactgcgtg atgccgctgc
    21481 tgctcatctt gagcagcagc tcaggacatt tattcgtctt cctccggaca gcccgcagcg
    21541 ggggaaaatg gcaaaagccg catatgacca gctcagactg tatctgatgc tgacgcaacc
    21601 gcaacatatg gagcccgcct ggttcagtcg tacgctcatg cgggaatggc cacagcgtga
    21661 cggtgtgagt gccgttttct ggcaggctaa tggcccgacg ttgctggcgt attatgcctc
    21721 cgggattatt actcatcctc agtggaagct gacggctgat gaagagctgg tcagccagag
    21781 cagaactctg ctgcttcgcc atctgggaac acagaacagc gatgccatgc tgtatcagaa
    21841 aatgctggca cgggtggcac atcagtttgc ggatatgcgt ctgacggata tgaccggtga
    21901 tactgatgtc tcccgcctct tttttacgga tgaagtggtt cccggtatgt ttacccgcca
    21961 ggcctgggaa gaggccgtat tgccgtcgat agacaccgtg attaatgagc gtcgtgaaga
    22021 aatggactgg gtgctgacgg atggcaggca gaaggcaccg tcaccggtat cgccggaagc
    22081 actcagacag cgcctgacaa cgcgctattt tgctgatttt ggtaatgcct ggctgaattt
    22141 tctgaacagt ctgcacttgc gtaaagctca gacgctgtct gatgtgacag agcaactgac
    22201 gctgatggct gatgtgcgcc agtcccccct ggttgcactg atgaatacgc tggcggttca
    22261 ggggcgtact ggtcagcccc gggaagcggt tactgattca ctggttaaat ctgccagaaa
    22321 cctgctttca caggaaaaac agccagtggc agtaccggaa agccggcttc acggtcctct
    22381 ggcgacgact ttcgggccgg tgcttgcact gatggataat cagaacaaca gtgcggatat
    22441 gctgaatctg cagacatatc tgacccgggt gacacaggtt cgtctgcgtc tgcaacagat
    22501 tgccggctct tctgacccgc aggccatgat gcagatgctg gcacagacag tattgcaggg
    22561 gaaatcggtt gatttaaccg atacccggga ttacggtagc ctgaccgctg ccggactcgg
    22621 acaggagtgg tatggcttcg gacagacggt ctttgttcgc ccgatggagc aggcgtggca
    22681 gcaggttctt acgcccgcgg cagaaagtct gaatgcccgg tggcgtactg cggtggtgga
    22741 tggctggaat aatgcatttt ccgggcgtta tccgtttaaa aatgtcagca gcgatgcttc
    22801 gcttcccctg ctggcaaaat acctgaatac ggacacggga cgtattgccc gtttcctgca
    22861 gaataacctg agtggtgtgt tgcacagtga aggcagtcgc tgggtacctg ataccattaa
    22921 tacccgggga ctgacattca accctgcgtt tctgaaagcc atcaataccc tgagtgaaat
    22981 agcggatgtg gcgttcacca caggtaacgc ggggctgcat tttgagctgc gcccgggaac
    23041 tgctgccggt gtgatgcaga cgacgctgat aaccgataat cagaaactga tttatgttaa
    23101 tcagatgccg gtatggaagc gatttacctg gccggctgat acggaagcac ctggcgccag
    23161 tttaagctgg gtaagcactc aggccggtac ccgtcagtat gcagacctgc cgggaagctg
    23221 ggggctgata cgtctgcttg agatggcccg acggaaagcc gcatcgggcg ttgccagtgg
    23281 ctggagcctg agctggcagg cgcaggacgg tcgtatgctg aattacacac tgcgaactga
    23341 agcgggggaa gggccgcttg tattgctgaa actccgcaat tttgttctgc cggaaacggt
    23401 tttcgaactc tccggtacgt cagcgtttac aggcaatgac gaagatgccg gggattccgt
    23461 ggaggagact gactgatggc ttccattcat tcgctcctca gtgcatgcca gacgacaccc
    23521 cgggatgtgg ctgaaccggc tcaggtgcgt attgcgctgt gggataaatg gcttgctccc
    23581 gtgacgccag ataatcccgc aggtgatgat gcggggtacg atgatgattt tcagcagatg
    23641 cgtgaagagg tcaataaact ctccggagcc gatgccggaa ttgtcagcca gcttgcagaa
    23701 aaattactga ccaccagaac caaagatatc cgggtggcga cctggtatat ctgggcccgt
    23761 ctgcggcagg atggtgaaaa agggctggcc gacggtctgg agctgctgac cggtttgttg
    23821 cagcgttttg gagagcacct gcatccacaa cggtcacggg ccaggaaggc ggcgctggag
    23881 tggttatgca gcgcccgcat actggacagt ctgtctctgt atccggaggt ggtgaaggcg
    23941 gataccctgc gcattgccgg ggcgctgtgg ctggcagaac agacctttac ggatgaggct
    24001 tccgccccgg tgctgaacgg gttatatcag gcacttgaaa atcgcctgat gaaggccggt
    24061 ggtgttgatg cagtggtgcc tcaggaggct gccgcgccgg caccgacggt aacatccggc
    24121 agcgtcatgg cattatctgc catcacctcc ggacaggaac tgctgtcaca ggcccgtgtt
    24181 ctggcaaaat atctgcgtga ccagcctgag ggctggctgg cggctcatcg actgatgaaa
    24241 agcgtccggc atgacacgct gcatcagtta ccgccgttat ctgctgatgg acggacccgt
    24301 attgccccac cggggccgga ccggcgtgcg tcactgaaac ggttgtatct gcagcagaac
    24361 tggttgtcac tgcttgaaca gtgtgacgat atgtttgccc ggggagccag tcatctgtgg
    24421 ctggacctgc agtggtatat tcatcaggcg ctgttgcaga ccggaaaaga aaattatgct
    24481 gccattattc agtatgacct gaaaggattg cttctgcgcc tgccggggct tgagacactg
    24541 gcttttaacg acggcatgcc gtttgccgat gacgtgacgc tgagctggat acagcagcag
    24601 gtgatggagt gtggtgaacg ctgggcggaa gaaccttctg taacgataac ggcagctccc
    24661 ggagataacg acattttatc tcttgagccg gaagccctgc agattgcgga taatgaagga
    24721 acagaagccg ctttaagctg gttacaggca cgtcccggta ttcagtctga ccgcagcaac
    24781 tggctactgc gcctgttgat ggcccgtgtt gccgaacaga ctggaaaaaa tgaccttgcc
    24841 ctgcatctgc ttgtggagct ggatgaacgt gcaacccggc tgaccctgtc acaatgggaa
    24901 cctgaactgg tatttgaggt aaaagcccgc cgcctgaaac tgttacgtat gaagtccgca
    24961 aaaactgaaa gtgacagggt gcgtctgcag cctgatatgg aacatcttct tgccgggctc
    25021 atcgccattg atgctgccag agctgctgtg ttgtgcaatt cgggaagttc atgagatgtc
    25081 tgaattatat tcatgtgtaa tctgattaat gaaatcctgc ctgcagttca gttcggacga
    25141 ttgtttgaaa tatggctgaa gtcagagcgc gagtctccgg aacagttagc tttattctga
    25201 gaagataatg gacgatttaa ccctgcgtta ttttgacgct gaaatgcgtt atctgcgtga
    25261 agccggaaag gcgtttgcac aggctcatcc cgatcgggcg gcgatgctgg atctcgataa
    25321 agcgggtacg cctgatccct gcgtggaacg cctgtttgaa ggtttcgcct tttccatggg
    25381 acggctgcgc cagaaaatcg atgatgattt gccggagctt actgaaagtc tggtcagtat
    25441 gctctggcct cattacctgc gtactatccc gtccctgtcg gtggtggcac tgactccccg
    25501 gttatccgtc atgaaaatgg ctgaaaccgt accagccgga ctggaagtga cttcccgccc
    25561 tgttggcccc ggaaatacag tctgtcgtta ccgtaccacc cgggccatac cactgaatcc
    25621 actggcagtg gagaaggtcg ttatgactac ggagccggac gggcgctcag tgctgaaaat
    25681 aggttttgcc tgcagtgaac tggctgactg gtctcaggtc gatttgcatc gtctgagcct
    25741 gtatctggca gcagaagccc cggtcagcag cacccttcat ctgatgatga caaagcgtct
    25801 ggctgcgctg tatctgaggc tgccgggaaa tgatgagcgg atccgcattg acggatggtt
    25861 ttcacccggt ggttttgcag aagaagatcg gctgtggccg aaaggtgaca gtgctttcag
    25921 tgggtatcag ttattgctgg aatatttcac ctttcgggaa aaatttatgt ttgttcatct
    25981 gaacggactg gagaatattt ctttgccggc aggtatttcc ggatttgata ttgaggtggt
    26041 gctgagtcag tcctggccgg cagatttacc tgtgacagat gatgccctgt gtctgcattg
    26101 tgtgccggtg attaaccttt ttacgcttga agccgatcca ctcatcatca atgggctgga
    26161 gagcgaatat ctgctgcgcc cgaagcgtct gcaggatgga tatacggaga tatattcagt
    26221 ggatgcggtg gcgggctccg ggcgaaccgg cagtgcagag tacgtgcctt ttaccagctt
    26281 ccgtcatcgt gggggaatgc tgcgccatga tgcacccgaa cgttattatc atacccgggt
    26341 gaagcggggt gtcacgggga tgtatgacac atggcttatc cttggcggtc agcggtggga
    26401 ggtggatcgg atgccggagc gggaaacgct ttcattacgt attacaggta ccaatggtca
    26461 gcttccccgt cgtgcgttgc agagtacgct gcttgatcgg tgtgaacagg tactgcagac
    26521 cccggtcagt gtatgtaatc tttgtaaacc cacgctgccg gtttatccgc cgacagaaga
    26581 tcgttttcac tggcgggtga tgagtcatct gggaacagga tttcttaaca tgctgagcag
    26641 tgcggaagtg ctgcgtggca cgctggcgct ttataactgg cgggacgatg aacttaatca
    26701 ccgtcggctt gatgccattc tggctgtaca gcatcatcgt attcagcgtt ttgaaaaggg
    26761 ttttctgctc cgggggcttg atgtggaggt aactcttgat ggcaacgggt ttgccggaga
    26821 gggagatatt cacctgtttg gcgaaatgct caaccgtttc ctggccctgt acgccgatat
    26881 gaatcagttc aatcagctca cactgattgt tcagcctgaa ggaaaatgca tccggtggaa
    26941 agaaaatcac aatccgcgcc tgcccggtta attacccgtt accggaaaca gcttccgtat
    27001 attaattttt accgcttctg tcagttactg gaacaaagcc agccagatca gcctcccatt
    27061 ggcagtggct ggcaggcccg tcaggaagcg gttcgctttt gtccataccc gggaatgggg
    27121 tttcctgcga gtgaaataaa agatgctgta attcctgaag aatcccatct gccacccact
    27181 gtacatgtga cgtttatggg actttacggg gtcacgtctc cgttgccggc tcactatatc
    27241 agtgatattg cacagcagcg ggaaggccat gaagccgcag cggattttct cgatatattc
    27301 agtcatcgtc tgatcaccca gtattaccgt atctggcgca aatattcgta cccggccacc
    27361 tttgaggcgg gggggcagga taaaacatcc cagtatctgc tcggtctggc cagactgggg
    27421 atccccggat gtgcacagaa tattgccacg ccggtatcgc gttttctggc gttattgccc
    27481 ctgatgttgt tacccggaag aacaactgaa gggctgacat cactggtaac gctgctggct
    27541 cccggaacac aggcccgtgt ctggcatcat gaccgacgac ggatcccgct gaaaacaccg
    27601 ctggccatgc gtgttcatca tccggtcagt ctgaagagtc gcccggtgat gggcgatcat
    27661 gctacggatg tgaacggaca ggtgttatta cagctttcaa cacagacggg cagcgaggta
    27721 cagggctggt taccgggcgg gcagctgtat tctgatttgc tggcgttgtt gcacgtttat
    27781 ctgggaagcc gtctggatgt acgtctgcaa ctttgtgttg agcgttcact gttgccggat
    27841 gtccggctca gttgccgacc ggcagccggt agtccgcaac tggggcgcac ggctgtaatg
    27901 agaacacagg caaagataac gacttcagcg gcacgcgtaa tgaccatccg tctggggcgt
    27961 tatcagcggg ttcaggaaca ttatcaacgt aaggaggctc aggaaaatgg cgattatcgc
    28021 tggtaaggct ggttacggtc tgattattgc gttgttttcc ctgtcgttat cagggtgtgg
    28081 cctgacgcaa agagtggcag acggtacggt atctgccacg aaatccctgt tttaccgaca
    28141 gataaaaaca cttcaccttg atattcgtgc cagggaggcc attaacacca gtgctgccgg
    28201 tattcccctg tcggttgtgg tgcggattta tcagcttaaa gacaaccgga attttgacag
    28261 cgcggattat caggcgcttt ttacggggga cagtgaaatt ctggcaggcg atatcattgc
    28321 acagaaagat gtatggatgc agccaggtgg ctcagtaacc gtggatatgc ctctggatga
    28381 tgcggcgaaa tttaccggtg tggcagcaat gtttctggag cctgaccgga agaaaaatac
    28441 ctggcgggtg gtgctgagcc gggatgagct tgagccggac acgccccgtc ttattgaagt
    28501 atccggtaat accctgacgc tgttaccggt gaaggataaa tagtgatgcc gcgtccttcc
    28561 ctttatgaaa ttctctatgg caatttcgct ggcgggctgg agttaaacca ggtcagtgcg
    28621 gaagagcagg ttattctttc ggtgctcgat aacatgcagc gtattctgaa tacccgggcc
    28681 ggaagcctta aacatctgcc ggattatggt ctgccggata tgacaaccgt tctgcaggga
    28741 atgccgggaa cggcgcatca actgatgcgt gttctttcag atgttctgct gaagtatgag
    28801 ccacgtatta agcgtgtcaa cgtgacgatg caggagcaga ctcagcctgg tgagttgcat
    28861 tatgtcattg atgcagaact taaagatgcc gggctggttc gttacggaac aacatttata
    28921 ccggagggca gggtgttgtt gcgccatctg aaacagcagc ggtacgttca gacgtgagcc
    28981 ggagcaggga gggcagaaca gaaataaaca gtgatggata tatgtcaggt gtgaatgtga
    29041 cattattccg gcttatgaca acgctggcgg aatgtttcat catcctgata actctggcat
    29101 atcagcaata acaatgtatg agtttttaaa tgaagaaaat tatctgtttt ctgttcgtgg
    29161 ggctaatgag atggtcaccc ccaccttaac agcagtacgc tgttaaggtg aatcccttta
    29221 acggagagac catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct
    29281 tccacattca ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta
    29341 ctaaactgat gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg
    29401 cgggtgcaca ctttatggct cgtcgtatct ctgattttgg gcatgaaact aagctaattt
    29461 ctccacagtt cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag
    29521 ccatatgcga agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagcccc
    29581 tgcaggccat gagggcgtta caccgggtcc gagaatcact catcagagat aaggtgaaaa
    29641 caaccaacca gatccacggt ttcttgctgg aatttggaat cagcctgccg acaggtgatg
    29701 ctgtaataaa acggttgtct ctcgtgttgg ccgaacacga aatccccgaa tatctgagcc
    29761 gccttttggt gaggttgcat acccactacc tttaccttgt tgagcaaata gcagagctgg
    29821 aatcagaatt aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc
    29881 ctggtgttgg gccgattact gccagcttac tatcgtcgca acctggtgac gggaaacagt
    29941 tttcatgcag cagagatttt gcggcttcca cagggctcgt tccccggcag tatagcacgg
    30001 gaggaaaatc gactctgttg ggtatcagta agcgaggcga taaaaatttg cgccggttac
    30061 tggttcagtg tgcccgctca ttcatgatgc ggcttgaacg ccagcatgga aaacttgccg
    30121 aatgggttcg ggagcaactc aacaagaagc attcgaatgt ggttgcctgc gctctggcaa
    30181 ataaactggc tcgaattgcc tgggtaatca ctactcagca aaaagaatat caggcttgat
    30241 ataaatgctg atataactca attggcttat tgtaaacaat aagatacaaa gttgcctgtc
    30301 tggttttgcg aagactgatt attgatgaca tgaacggcaa atcgacctga ttaaaaacct
    30361 aataaataaa atggctcact gaagccgagg ggctttttag gattatcagg tgcgaaactc
    30421 atcggggcgc aggcatcctt gcctacagag acgccggata gatttaagca agcccaccat
    30481 taattaaaaa atcagtgttg caaaaacggg ggcgaccata gattattttc ggttcagaca
    30541 gatgctgcgc ggggaagaaa accctgctcg ggtgctaaag gtggtatttc acactgcacg
    30601 tcagacggac gttttgtctg caatgacgga agtctgagtc agtcgaagcg cttttgttcc
    30661 ggttatggcg tggcacccgc cagacgtcag gtgaaatcac cagtcgccac taagccggtt
    30721 cagaagaaaa aatcagcggc tgtccggaag gtgtcacaac cgttagtggc tgagaaaaat
    30781 gaggttgtac cggtcactga accccgtaaa cccacctgtg cgccgcttta tatggccagc
    30841 aaacccggat ttacgcatct gcccatttgt tctggtaatc agtattaatc tgtcactgaa
    30901 atagcagagg aatctgtgct caggctgcag ttcatcctgt acttgcgggc atcataaagt
    30961 cggacaacct tccggatata aatatttccg gagagcgtta atctgagata ttcagttact
    31021 gagaaggaaa ataaaggaga gtcatggcag gaataaaaaa gataataatg cagcgggatt
    31081 tgtcctgatt ttagtttgcg ttattctctg ggggatttat gtggcggtca gagcgctgat
    31141 aaatcttaat gagcgcttta ttgatgcagt atcgaatcag acaggtatta ttggtttgtt
    31201 tttgggtttg cttactgtat ttgcaatttt acttcgtttt tttatttacc ggagactcag
    31261 aaagaaaaca gccgcctttg agcaggccgt ttcagcgctg gttcagagag aacgggattt
    31321 taatgaaact gtcaatgcca cgatagcccg gggaatacga caggaaaagg agcaacttgc
    31381 cagacgccgt gaggaatttc ataccgccag acagaaggcc agccgtgcaa tgcaacggat
    31441 tgttgattcc gcctggaaat ttaaggcgaa gactctgctg gctggtgtaa cgattaataa
    31501 ctggcaaagc aaatatgacc agcttcgtaa ggaaagagag gcttacgctg ctgtcagtga
    31561 gaaaatagcc tttcttaacc tggaagataa ttctgactgg gagagtgtca ggcaacagtt
    31621 tctggacaaa gtggcattgc tggaaaagcg caggaagaga aggaatatca ggcagagctt
    31681 aagcgtcaga tacgtgaaga aaaagagcgt caggatgaac ttgaccggcg ccagcgggaa
    31741 gcggaagagg aagaacggcg tcttgctgag cagcagaaac tgattgagga ggcgctgcgc
    31801 gcagcagaag gggctcacag ggaggagtta gagaaacaac gcctggagct ggaacagaaa
    31861 attcaggaag cgcatgcgca gtatgaacgt gctaaatcca tggctcagct tacgaaacag
    31921 gggcatgttt atatcatttc gaatattggt tcgtttggtg aggatgtctt taaaattggc
    31981 atgacccgac ggctggaacc catggacagg gtcaaagagt taagtggtgc ttctgttccg
    32041 tttgattttg atgttcacgc catgatttcc tgtgatgatg cgcctgcgct ggaaaaaaca
    32101 cttcatgaca gtctggaaaa ataccgcatt aacaggatta acctgcgtaa agaatttttc
    32161 cgcgtgaagc ttgagaaaat cattaatgaa gtggagcgtc accacgggca gatggaatat
    32221 gtggctgacc cggcggcgct gcagtacctg cagagtctgg aatacgcaga aaatgaagca
    32281 gcctgacagt cagtgttctg attttacccc ggccggatat aacagaaggg atatctgcag
    32341 ataccctgca gcatggttca cagtattctg gtcatgttaa tgattctgtt ttttatttct
    32401 gaataaagcg acacccacga tataacatcc gtggatgtcg gtggtgatta ttttttgttc
    32461 acagattaag gtgcgtttat ggcgaaactg ataaatgtat cggcctccac tgaggagaga
    32521 atgacgcctg gggagaggag ggtggcttca cgactggaga gctttctgaa tgatgactgc
    32581 cttgtctggt acgatattcc ggtgggacgg aaaaaccggc atccggattt tgtcattatt
    32641 gacccggaga acggactggt atttctggag gtcaaggact ggacggtttc cacgctgcgc
    32701 caggtaaatc aggagcaggt cacgctggaa accgacggtc ttctgaaatc agaaatcaat
    32761 ccgctggtac aggttcgtcg ttatgcctgt gatacggtta atgccttacc tgcaaacccg
    32821 tgcttacggc agaatgacgg tcagtataag ggacggctga acctggcctg ggcgtatggt
    32881 gtggtgttta cccgcattac ccgtcagcaa ctgaagacac tggcagggaa tgatgagaat
    32941 gcggtggaaa aaatttttcc ttcagcacag acaatctgcc aggatgaaat gacgcagtct
    33001 gttttacctg aggtattcca gcaaaaaatt gccgggatgt ttaccacggg attcagaacc
    33061 cgggtgacac cccggatgag agatattctg cgcgcccatc tgtttcctga agtgaccgtg
    33121 aaacagaact cacaaatcaa aatcatggat attcagcagg agattctggc cagaaatatt
    33181 ggtgacgggc atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat gattcttttg
    33241 ttccgttgcc tttacctggc tgaaaccaca cccggaaaga ttctggtgtt gtgctacaac
    33301 attaccctgg ccagctatct gcgggaatgc attgaagcca gagggttggc gtcacgggtt
    33361 acggtatccc attttcacag ctggtgtgcc tcaatggtaa aacgccacgg tattaaggtg
    33421 acggccgaca gaaaagaata tcctgataaa tgcttcagtg cactggaaga ggccgtgaac
    33481 agcggaaaaa tcacccgaac cggatatgat gctgtactgg tggatgaggg gcatgatttt
    33541 gacagtcgct ggctggccct gattgcccgt ctgtttgata acgcatcccg ttcactgctc
    33601 ctgatgtacg atgacgccca gtccatttac cggcgggagc gggccctgaa cttttctctg
    33661 gccagtgtgg ggattcaggc acagggacgc acttctgtcc tgccggtcaa ttatcgtaat
    33721 ccgaaacgca ttctgcactt tgcttatgca ttctcgcgcg agtattttga aaaacatcag
    33781 aaccgtgaaa taccgctcgt tcagccacag gcgggcggtg aagagggtac ggagccggaa
    33841 atcctgcgtt gtgcttctga acgtgatgaa gccagacaag ttgcagactg gctggagaag
    33901 cgccatgccc tgtgtggtca ctggagtgat atggctgtgc tgtgtccgac agagcgttca
    33961 gcgcaacaat tgcaggacgt tatgacacta cgcggggtgc cttttgccac ctgttttgac
    34021 agggaaggca agaagacata ctcgcgacgt aaggatgtgg tgcatttact gacgtaccag
    34081 agcagcaagg ggctggagtt cccgtatgtg gcggttatca atgcgtcttt cgttcactct
    34141 ggtgcagcgg atgagtctga agtgattccg gtgctttatg tggcgttcac ccgggcaacc
    34201 cgtgaattac tggtgacctg ttaccgtgaa aacagtatca gtcgtcacct tgaggatttt
    34261 gcataatacc ggattatttc tttattccgg actttcatca tactgttatt atcaggttta
    34321 tttaattcgc catgaacccg ttatttaatg atattcagat gcgtcttttt tatctgaacc
    34381 actctccgta ctcctggcac tggaatgtca gattcagacc ccaggaagct gtctatattg
    34441 gcagcgatac ctgtcatatc accattacct gtaatcagtc cggttttcat ttaaccaggg
    34501 acggacaacg gctgttcact gaacgttata tcagaaacct taatgagctg ttaccggttc
    34561 tgaaacggcg gtgggatgtg acaccggcaa tcatccgtgc cgtggaatac ctttcccggg
    34621 tcccggtatt acactgacat tcttttcgtc actgttaatc atgctttaat acagtaacac
    34681 cgaatctgcc gatgacttct gaagtgaaac tgaaaaccgg tggtgacccc aggtcattac
    34741 cggattatgc tgccctgcgt gatgaaatca gcaaactgac ccacccggca cgtccggatg
    34801 tggactggcg ttacgtggaa acgctctgcc tgcgtctgta tgaacacaat ggcgtggaat
    34861 tacagacggc atcctggtac accatcgccc ggatgcatac agcagggtta agtggactga
    34921 atgaagggct ggctctgatt gtggcgctga cccggcatca ctggtcagtg atgtggccgc
    34981 tgaataccca cgcccggctg gaaattatta ccggtctgtt caaccgtctg caaaaaacgc
    35041 ttcgggccat gcctccggac gaccgtgaca acctgcccct gctgtatcag accgaaacat
    35101 tcctgaaggc actgagtgac acacttgccc ggcacgaact taaacagggc agtaaagtgg
    35161 ctttaccgga agccatggtg aagggatata tcacccgcct ggaaaatcag cctgtgcagg
    35221 gcgaagcgtc gtctccggta acgcttccgg ctcaggcact ccggtctgat gttccggatg
    35281 tgcaggaaca tcagaccctg ccgcactccc gtctggtgta tgttgtttct gaaacaaaag
    35341 cagagcccac accctccctt cagaaatccc cgcccatgtt tctgaagccg ttttgggctg
    35401 gtgtctgtgc ggcattgctg gctgtcagcg tggccattcc gggatggcag tttctgacac
    35461 agccatcacc ggcggagcag caactcaggg cgctgctgac acccccaccg gatgtgcttt
    35521 cggcagacca gatagcacag ctcagcagaa cacgctcact gttaaatcat acgtcagagt
    35581 ggatacccct ttccggtcaa cagataacgc gactgacaga attacccctg gcatacaacc
    35641 tgcagcgcag tgcgcagctg ctgcagcaac tgagggtact tttccccgat aatccccggg
    35701 tacaggaaat ggtggataac tggcagaaga gtgtaaggag ccgtgcgctc ccggaagagg
    35761 cgatgacggg ctggaacgaa ggcatgaccc gcctacagca gctggctgag cgcctgaacc
    35821 gtctggatga acagcgggga aaatacatga cagtcagtga actgaaaaca gaggtgtttg
    35881 gcatcatgca ggcttttaac cggcatatcc cggcagaaga gcagttacgt cgctataatg
    35941 aagtccgcaa ccagaatggc agtgaacaac agcaaaaact gacgcaggat gccctgacgg
    36001 aactgttgaa ccgctactgg tttctgagac atggagatgc aggtaagaag acgataaata
    36061 ataaacagga taaccagatt aatatctgac agattttatt gccattattt atccagaagc
    36121 gatgatgcca ttaatgcaaa atgacatctg aattgatcac agattggacc aatctgtgat
    36181 caaatagaac agaatacgca gatgataaag cggctttccc tagaagattc acaacgtcat
    36241 ctggcagaat atgggtgaga gttgcaggct gatgcgtaca aagcttacga tgcgttgtat
    36301 gaaactggcc gggtgaaaga tgtcatccgt acggtgcgcg ccagactaaa caggcatcat
    36361 gatggaaaag tcgggtatca ttcacaggca tatttgtcag aatggtagcg tcagcgggtt
    36421 ttgattacac cagttttgaa gctcatactt cctatgaggc actgcagaaa acgaaagtgt
    36481 cgtttaggcg ttgggggagg ggttaaaaaa gtaacggcag aagtcaccct gaagttgagg
    36541 cagtcaacta ctgttagctg gctgctgcat gagagtgtgg agggagattg tatatattgg
    36601 ttagtcagag tctgcttcgg aataagtatg agtaattttt aaaatgggcg gaagttattt
    36661 ttaattactg ggctatgtaa caaatgcaga ttcctgtcag aaacatgtta taaggaaata
    36721 gtaatatggc caaaatgatt ccggcatttg gacccattga taataatagt tgtggggaaa
    36781 aaacagtcta tacactgtta aaagaaggtc tgtccgatga tttcacagtg attcatagtc
    36841 tcccgtggtt aagtgcggcg acacgaaatt tagggatgaa attacctccg agtggagaaa
    36901 ttgattttct tgttctccat cctgtatatg gtgttttggc gttagaggta aagagtggaa
    36961 tatatcgcgt agaaggtact gtttttgtac atataaaaac aaaaaaacat gtagatatag
    37021 tgaagcaaac acgtaacaat gtccatggtt tagcccgttg gttaggggga gcaacaacgt
    37081 tacggttgcg tattggttat ggttttattt ttcctgatag ttatttcgat gataaaatta
    37141 taaatgcagc aatggtagat gcgtctgtta aaccattcag gggaatattt gttgataaaa
    37201 gccaactccc ggaaatggca aagcatacta ttgatataat gcaatactgg aaacatgcat
    37261 tgggaaataa tgagttgggg gatgaacggg ttcaactaat aataaaatct gtatgtcctg
    37321 agtttgatgg tactcctgac tggggaatcc gtattattta tgataataaa ttatggttaa
    37381 ggctcacaca tgaacaaatc aaggtcattg aacttctgtc agaatatcaa agagttgttg
    37441 taaccgggtg gccaggaaca ggaaagaccc tgattggaat tgagtttgca cggcggcttg
    37501 ttaaaggcca aaaaaaggtt ttatttatta ctttcaataa ccttctttca gaatatattc
    37561 ggcaacaaac tccggagtca ttatgcgacg tttttacctg gcacaaactt tgtcatcagg
    37621 ctcgaaacaa attatccctg tcccccgagt ctccggcagg ctggtttgaa tcaggatgct
    37681 gcgaagattt gcttaatgca ttaaataaaa ataaactaag agattatgat gctttaatca
    37741 ttgatgaagc acaggcgtta cattcttcgt ggctggagac tttatcttgt tggttcgaaa
    37801 ataaaaggat tatcgcattt tgtgatgaaa ctcaaacatt ttcctttgaa aaaggaacaa
    37861 attacgctga tcttcagcga ataataaatc cccaattttc ttttaactta tctgttgtgt
    37921 taagatgtcc tagagccgtt actgattatt taatagctgt cagccctgcc tcataccaaa
    37981 ttacttcccc taggaataac gaaccggata cgctaaaaga attagttgtt acagatccac
    38041 tctccacatt gcttaatgaa attttacaat tgaaagaaag ttctgtactg cctgatgaga
    38101 ttactgtcct tgttcctacg gatttagtga aaaattccct ccagaatgat atgagaactt
    38161 tgggggtgaa tattgaaaca gtatctcgtt ttcgaggtat ggaatcatct gtcgtgatta
    38221 cattgtattc cgaaagcatg gaggatgcac agctattttg tgcttattca agagctacaa
    38281 ctgcttttat tgcaatttat gattcggaga aattagcatg gagagaaaaa ataaattttt
    38341 gcataaactg gttaacagaa aggaggttca gagagcaatt aatgaagcaa agcataattc
    38401 ttcctccagt gttcttatgg ccggtttttt ccgcgaagga gagttaaaac ttagtactgt
    38461 aaaattagca tggtcgaagc acttttcttc gtggcttgtt gaatttgatg aaagaaattc
    38521 tccagaagaa atgtggatag attatttgta ttcctatttg ccttacccga ttatata
//
LOCUS       DAZFFI010000035        37942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFFI010000035 DAZFFI010000000
VERSION     DAZFFI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.035"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..258
                     /locus_tag="ACHJ12_002105"
     CDS             <1..258
                     /locus_tag="ACHJ12_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO4989886.1"
                     /translation="PDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQHAYHA
                     ATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILF"
     gene            336..1544
                     /locus_tag="ACHJ12_002106"
                     /pseudo
     CDS             336..1544
                     /locus_tag="ACHJ12_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1713..2132
                     /locus_tag="ACHJ12_002107"
     CDS             1713..2132
                     /locus_tag="ACHJ12_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4989887.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRISRRLPVTTTSDAGLELLPVEITSDEPKEPMAALTPPLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            2129..2479
                     /gene="tnpB"
                     /locus_tag="ACHJ12_002108"
     CDS             2129..2479
                     /gene="tnpB"
                     /locus_tag="ACHJ12_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4989888.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRWGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            2510..4102
                     /locus_tag="ACHJ12_002109"
     CDS             2510..4102
                     /locus_tag="ACHJ12_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO4989889.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIHRLESEESCCPECGGELDYLGEVSTEQLELVSSALK
                     VIRTERVKKACTKCDCIIEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNTRKLHTDDTPVKVLAPGLKKTK
                     TGRIWTYVRDDRNAGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLF
                     SAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEIRGLPESE
                     RLAVRQQRSKALLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAET
                     DNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSV
                     LPEWPSNRVDELLPWNVVLTNK"
     gene            complement(4391..5575)
                     /locus_tag="ACHJ12_002110"
     CDS             complement(4391..5575)
                     /locus_tag="ACHJ12_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4989890.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(5875..5969)
                     /locus_tag="ACHJ12_002111"
     tRNA            complement(5875..5969)
                     /locus_tag="ACHJ12_002111"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5933..5935),aa:Sec,seq:tca)
     gene            6262..7644
                     /gene="yicJ"
                     /locus_tag="ACHJ12_002112"
     CDS             6262..7644
                     /gene="yicJ"
                     /locus_tag="ACHJ12_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4989891.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7654..9972
                     /gene="yicI"
                     /locus_tag="ACHJ12_002113"
     CDS             7654..9972
                     /gene="yicI"
                     /locus_tag="ACHJ12_002113"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4989892.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(10025..11734)
                     /gene="yicH"
                     /locus_tag="ACHJ12_002114"
     CDS             complement(10025..11734)
                     /gene="yicH"
                     /locus_tag="ACHJ12_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4989893.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(11855..13246)
                     /gene="xanP"
                     /locus_tag="ACHJ12_002115"
     CDS             complement(11855..13246)
                     /gene="xanP"
                     /locus_tag="ACHJ12_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4989894.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            13526..14731
                     /gene="gltS"
                     /locus_tag="ACHJ12_002116"
     CDS             13526..14731
                     /gene="gltS"
                     /locus_tag="ACHJ12_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4989895.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            14734..15600
                     /locus_tag="ACHJ12_002117"
     CDS             14734..15600
                     /locus_tag="ACHJ12_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989896.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(15585..17666)
                     /gene="recG"
                     /locus_tag="ACHJ12_002118"
     CDS             complement(15585..17666)
                     /gene="recG"
                     /locus_tag="ACHJ12_002118"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4989897.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17672..18361)
                     /gene="trmH"
                     /locus_tag="ACHJ12_002119"
     CDS             complement(17672..18361)
                     /gene="trmH"
                     /locus_tag="ACHJ12_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4989898.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(18368..20476)
                     /gene="spoT"
                     /locus_tag="ACHJ12_002120"
     CDS             complement(18368..20476)
                     /gene="spoT"
                     /locus_tag="ACHJ12_002120"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4989899.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(20495..20770)
                     /gene="rpoZ"
                     /locus_tag="ACHJ12_002121"
     CDS             complement(20495..20770)
                     /gene="rpoZ"
                     /locus_tag="ACHJ12_002121"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4989900.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(20825..21448)
                     /gene="gmk"
                     /locus_tag="ACHJ12_002122"
     CDS             complement(20825..21448)
                     /gene="gmk"
                     /locus_tag="ACHJ12_002122"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4989901.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            21706..23388
                     /gene="ligB"
                     /locus_tag="ACHJ12_002123"
     CDS             21706..23388
                     /gene="ligB"
                     /locus_tag="ACHJ12_002123"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4989902.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(23385..24002)
                     /gene="yicG"
                     /locus_tag="ACHJ12_002124"
     CDS             complement(23385..24002)
                     /gene="yicG"
                     /locus_tag="ACHJ12_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4989903.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(24294..25118)
                     /gene="dinD"
                     /locus_tag="ACHJ12_002125"
     CDS             complement(24294..25118)
                     /gene="dinD"
                     /locus_tag="ACHJ12_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4989904.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(25339..26202)
                     /gene="yicC"
                     /locus_tag="ACHJ12_002126"
     CDS             complement(25339..26202)
                     /gene="yicC"
                     /locus_tag="ACHJ12_002126"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4989905.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            26329..27045
                     /gene="rph"
                     /locus_tag="ACHJ12_002127"
     CDS             26329..27045
                     /gene="rph"
                     /locus_tag="ACHJ12_002127"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4989906.1"
                     /translation="MRPAGRSNNQVRPITLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            27111..27752
                     /gene="pyrE"
                     /locus_tag="ACHJ12_002128"
     CDS             27111..27752
                     /gene="pyrE"
                     /locus_tag="ACHJ12_002128"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4989907.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(27789..28385)
                     /gene="slmA"
                     /locus_tag="ACHJ12_002129"
     CDS             complement(27789..28385)
                     /gene="slmA"
                     /locus_tag="ACHJ12_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4989908.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(28492..28950)
                     /gene="dut"
                     /locus_tag="ACHJ12_002130"
     CDS             complement(28492..28950)
                     /gene="dut"
                     /locus_tag="ACHJ12_002130"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4989909.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(28928..30148)
                     /gene="coaBC"
                     /locus_tag="ACHJ12_002131"
     CDS             complement(28928..30148)
                     /gene="coaBC"
                     /locus_tag="ACHJ12_002131"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4989910.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            30320..30988
                     /gene="radC"
                     /locus_tag="ACHJ12_002132"
     CDS             30320..30988
                     /gene="radC"
                     /locus_tag="ACHJ12_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4989911.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            31205..31441
                     /gene="rpmB"
                     /locus_tag="ACHJ12_002133"
     CDS             31205..31441
                     /gene="rpmB"
                     /locus_tag="ACHJ12_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4989912.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            31462..31629
                     /gene="rpmG"
                     /locus_tag="ACHJ12_002134"
     CDS             31462..31629
                     /gene="rpmG"
                     /locus_tag="ACHJ12_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4989913.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            31727..32536
                     /gene="mutM"
                     /locus_tag="ACHJ12_002135"
     CDS             31727..32536
                     /gene="mutM"
                     /locus_tag="ACHJ12_002135"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4989914.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(32575..33054)
                     /gene="coaD"
                     /locus_tag="ACHJ12_002136"
     CDS             complement(32575..33054)
                     /gene="coaD"
                     /locus_tag="ACHJ12_002136"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4989915.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(33062..34339)
                     /gene="waaA"
                     /locus_tag="ACHJ12_002137"
     CDS             complement(33062..34339)
                     /gene="waaA"
                     /locus_tag="ACHJ12_002137"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4989916.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            34752..35810
                     /gene="rfaQ"
                     /locus_tag="ACHJ12_002138"
     CDS             34752..35810
                     /gene="rfaQ"
                     /locus_tag="ACHJ12_002138"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4989917.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            35807..36931
                     /gene="waaG"
                     /locus_tag="ACHJ12_002139"
     CDS             35807..36931
                     /gene="waaG"
                     /locus_tag="ACHJ12_002139"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4989918.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            36924..37721
                     /gene="rfaP"
                     /locus_tag="ACHJ12_002140"
     CDS             36924..37721
                     /gene="rfaP"
                     /locus_tag="ACHJ12_002140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4989919.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         9173 a         9325 c        10071 g         9373 t
ORIGIN      
        1 ccggatgtca cactacatta tccgtatctg cgccggatgt cctgggcgca gataacggca
       61 gcagctgaag atgcggaaat acatcatgat tatgcccggg cattaatatt gtggcagcat
      121 gcatatcatg ctgccacgct gactctcaac aaaaacctgg cagttgctaa aatcgacttc
      181 tgtattaaac gtatccggat gcatgaacaa atgagcagga taatccggaa gactggcaca
      241 gacgaaatac tcttctgaac cggccccttg aaacgccaga cagtaaaata agagataggc
      301 ttggatatat gtctaacact agtgctaatt ttgagatgac tggaattttg ctggggcagg
      361 agcttcgtaa acgtaaaact cctcaggaaa aaatcgccat tattcagcag acaatggaac
      421 ccggtatgac ggtgtcccat gtggcgcgtt tgcatggtat tcagcccagc ctgttgttta
      481 agtggaaaaa aacaatatct ggaaggaagt ctcactgctg ttgctgccgg agaggatgtc
      541 gttcctgcct cagaactggc tgccgctata aaacaaataa aagagttaca gcgcttgctg
      601 ggtaagaaaa ccatggaagt tgagatcctc aaagaagcgg tggagtatgg tcagtcgcga
      661 aaatggatag cgcatgcgcc cttgttgcca acggacaagg aataaccctg gtaagccgta
      721 ttctgggcgt gtcgcgtgcg caactggcac aacggatgaa aacatctggt aataagccga
      781 ataaacgtcg tctcagatgt gatgaggctg cggatgcgga agtgttagca cgtatccttg
      841 atatcatcag cgatatgccg acatatggct atcgtcgtgt ctgggccatt ctcagacgac
      901 agtcccgtaa caaaggagtg ccgttcgtga atgcaaagcg cgtttatcgg ataatgagcg
      961 aaaataacct gttgttgtta catgacaaac cttcacggcc gcagcgggaa cataaaggcc
     1021 gaatatcggt gaaggaaagc gaccagcgct ggtgttccga tggctttgag ttcggttgtg
     1081 atgatggcgc aaaactgcgt gtcacgttcg cactggactg ttgcgatcgt gaagccatag
     1141 actgggccgc aagcacggga ggttatgaca aagcaacggt gcaggatgtc atgttaggag
     1201 caatagaaaa acgctttggc gataaaggag cggaacggcc aatccagtgg ctgactgata
     1261 acggttcggc ttatacagct catgaaacac ggcagttcgc cagagagttg aatctggagc
     1321 cctgcacgac ggcagtcagt agccctcaga gcaatggaat ggcagaaagg ttcgtgaaaa
     1381 cgatgaaaga agactacatc gcgttcatgc cgaaaccgaa tgtaagaacg gcattgcata
     1441 accttgcagt ggcgatcgag cattacaatg aaacccatcc gcatagtgcg ttaggttatc
     1501 tctcaccaag ggaatatcga cgtcagcggg taacgtcaac ttaagataca acgactgtct
     1561 gaatatcgtg ggtcaaggac actttccttg agaacaataa ttttatcttt atgtgaatct
     1621 catttttgta agcgtaaacc gaccgccgta tgtagccatt agacaagaat tggtaactta
     1681 gacgcccatc tgacacagac ggacatctaa gtatggaatt acaggactgg cgaaaagaac
     1741 ctcgtaaaaa gtattcgaat gaattcaaac ttcgtatggt tgaactggca tcacaacccg
     1801 gtgcttgtgt ggctcagatt gcacgagaaa atggcgtcaa tgataatgtt attttcaaat
     1861 ggctcagact ctggcaaaac gaagggcgta tatcacggcg tcttccggtg acaaccactt
     1921 ctgacgcagg ccttgaatta ttacctgtag agataacctc tgatgagccg aaagaaccga
     1981 tggctgctct tactccgccc ttatccactc agactactgt cagtgccagc tcctgcaagg
     2041 tggagttccg tcacggtaac atgacgctgg aaaatccttc accagagctg ctcactgtat
     2101 tgattcgtga actgaccggg aggggaagat gatctcactc ccatccggta cccgtatctg
     2161 gctcgttgcc gggataaccg atatgcgtaa gtctttcaac ggactgggag aacaggtaca
     2221 acatgtgctg aatgataatc ccttctccgg tcacctgttt atcttccgtg gccgatgggg
     2281 tgacaccgtc aaaattcttt gggctgatgc tgatggtctg tgcctgttca ccaaacgcct
     2341 ggaggaaggc cagtttatct ggcctgcggt acgtgacggc aaggtatcca ttacccgctc
     2401 gcaactggca atgctcctcg ataagctgga ctggcgtcag ccaaaaacat cccgccttaa
     2461 cgccctgaca atgttgtaaa aaacgcctgg ccgcattata aaaacggcca tgagtcagaa
     2521 atacctcatt cgcattgcag aactggaaag ccagctccgt cagaaagacc agcaactgag
     2581 tctggttgaa gagacggagg ccttcctgcg ctctgcactg gcacgtgccg aagaaaagat
     2641 cgaagaagat gaacgggaaa tagaacatct gcgggctcag atagaaaaac tgcgccggat
     2701 gctgttcggt acccgttctg aaaaactgcg tcgtgaagtt gaacaggctg aggccctgct
     2761 gaaacaacgc gaacaggaca gcgatcgtta cagtgggcgg gaagacgatc cacaggttcc
     2821 ccgccagttg cgacagtcgc gccatcgtcg tccgttaccg gcacaccttc cccgtgaaat
     2881 acaccgtctg gagtctgaag aaagttgttg cccggaatgt ggcggcgagc tggattatct
     2941 gggggaagtc agtactgagc aactggagct ggtgagcagc gccctgaaag tgatccgcac
     3001 agaacgggta aaaaaagcct gtacaaaatg tgactgcatc attgaagcac cggcgccgtc
     3061 ccgcccgata gagcgtggta tcgcgggccc cggattactt gcccgcgtgt taacgggaaa
     3121 atactgcgaa cacctgccac tgtatcgtca gagtgaaatc tttgcccgac agggtgtcga
     3181 actgagccgt gcattactct ccaactgggt tgacgcgtgc tgccagttaa tgacgccgct
     3241 gaatgatgcc ctgtaccgtt atgtgatgaa tacccgcaag cttcacactg acgacacacc
     3301 ggtaaaggta ctggcaccgg gcctgaaaaa gacgaaaaca gggcgcatct ggacgtatgt
     3361 ccgggatgat cgcaatgcgg gttcgtcatc tcctccggcg gtctggttcg cgtactcacc
     3421 gaaccggcag gggaaacacc cggagcaaca cctccgcccc ttccggggta tcctgcaggc
     3481 ggatgcgttc acaggttatg acaggctgtt cagtgcagaa cgtgaaggtg gtgcgctgac
     3541 agaagttgcg tgctgggccc atgcccggag aaaaatccac gatgtataca tcagcagcaa
     3601 aagtgcgacg gcagaagaag ccctgaagcg aatcagtgaa ctgtacgcca tcgaggatga
     3661 aatacgggga ttaccagagt cagagcgtct tgcagtcaga cagcagcgaa gcaaagcgtt
     3721 actgacgtcg ctgcatgaat ggatggtgga gaagaatggc acgctgtcga aaaaatccag
     3781 actgggcgaa gcgttcagct atgtactgaa tcagtgggac gccctctgtt attacagtga
     3841 tgacggactg gcggagacgg acaataacac agcggaaaga gcgcttcgtg cagtctgtct
     3901 tggaaagaaa aattacgtgt tcttcggtag cgatcacggt ggcgagcgtg gtgcactgct
     3961 atacgggctg atcggcacct gccgtctgaa cggtatcgat ccggaagcgt atctgcgcca
     4021 tattctgagc gtactgccgg aatggccctc caaccgtgtt gacgaactcc tgccatggaa
     4081 cgtggttctc accaataaat aagcgtcaat acggtgctcc gttgatgctt actcattttt
     4141 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     4201 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     4261 gcttttaatt atgtttttgt aataagtctg aagacgcaaa aaataatata ataacaaaaa
     4321 tcctccagcc ttgtaggcca gaggataaaa tacttaattg aggaaaacat cagggaaaag
     4381 tatttctgac ttaactttcc cggctggaga gtgaatcaat gtaatcagcg taccattgca
     4441 tcatttcacg gcggccatcg agatacaggg catgattata ggtacccctg atggaatttt
     4501 tatccacatg agctaactgc atttcaatcc aggcggaatt aaagccttgt tcatgcagaa
     4561 ttgtactcat ggtgtgtctg aaaccgtgcc ctgtgagtct tcccttatat ccaagtaatt
     4621 caattacttg gttaatgctt tccttactaa ttggtttgcg gggatcattt ctaccgataa
     4681 agactagggg atgatgtttt gagattggtt caagctgctt aaatagcata atgacctgct
     4741 cagagagcgg cacaatatga ggtcgcttca tcttcattac ttctgccggg atctcccaca
     4801 attttgtctc aaaatcgata tcttcccagc gtgcaaaacg cagttcttgt gttcgtacgc
     4861 cggttaacat aatgatctga gtagctgtct tagtgattat acttccggta taacccgcca
     4921 gatcgttgag gaagtgggga agttcattag cagtaagaaa gggaaaatgt acttttttcg
     4981 gtgtagctaa agcactggca agatcggggg ctgggttata gtctgcccgg ccagtaacaa
     5041 ttgcatagcg gaacacctca ccacagcgct ggcggacctt gcgcattttc tccagagctc
     5101 cacgtttttc cattttacgc agtgcttcca gtaactccat tggtttgatt tcggcaatag
     5161 ggcgcttgcc aatgtaggga aaaatgtctt tttcaaaggt atcaatgatt tcatcacgat
     5221 agcgtaatga ccaccgatcg gcttttgact ggtgccattc gcgagcaata gcctcgaacg
     5281 tatttttcag actcatttgc tgcgcgattt tttcttcctt ttttgcctcc cctggatcgc
     5341 ctccagcagc gaggagtttc ctggcttcac tgcgtttctc gcgagcatcg gctagcgtta
     5401 catcggggta tacgccaata gaaagcatct tttctttacc agcaaagcgg tacttcatgc
     5461 gccagtattt agaaccatta gtgttaatca gtagatacat accgccgcca tcagcaagct
     5521 tatagggttt gtctttgggt tttgcggttt tcactttgat atcggttaat gccatgacat
     5581 cctccggctt ttgatgaatt catgttcctg agggggattt ctgccagtaa ctaacctgat
     5641 acccccaata caccccttgt ataacaaaga tgttgttaaa agccgttgtt atatatcaca
     5701 tttttcctat tatggggggg ctggtattta aataggaaat aacaccccct aaagtacccc
     5761 caaaagaaac ttgatacggg gtgaagtagg ttgacctctg gatacaaata aatactgata
     5821 tacaagggat tagaagagat ttattgacat tgggagagtt cagaagaggt aaactggcgg
     5881 aagatcacag gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc
     5941 agccgcctca ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc
     6001 attatagcta cttccttgag tttctacatc ccccagatca gttttttccg cctaatacac
     6061 gcagcgtatt atcacgaaag cattatagtt catataaaac ccatatttta caatgatata
     6121 tgagcggaat taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat
     6181 atcggaatat tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc
     6241 tatagcatca aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt
     6301 tatggcatgg gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg
     6361 ttcttttata ccgatatttt tggcattcct gccggatttg tcggaaccat gtttttggtc
     6421 gctcgtgcac tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc
     6481 tctcgctggg gtaaatttcg tccgtgggta ctgtttggcg cactgccatt cgggatcgtc
     6541 tgtgtactgg cctatagcac gccagatctc agtatgaacg gcaaaatgat ctatgcagca
     6601 attacttaca ccctacttac cttactttat accgtcgtca atatccctta ctgcgcattg
     6661 ggtggtgtaa tcaccaatga cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg
     6721 ctggcgaccg cgggaggcat gctttctact gttctgatga tgccactggt taatttaatt
     6781 ggcggtgata ataaaccact cggtttccag ggcggtatcg cggtcctttc cgtggtggca
     6841 ttcatgatgc tggcattttg tttcttcacc actaaagaac gcgttgaagc accacctaca
     6901 acaacgtcta tgcgggaaga tttacgtgat atctggcaaa acgaccagtg gcggattgtc
     6961 ggtttactaa ccattttcaa tatcctggcg gtgtgcgtac gcggtggggc gatgatgtat
     7021 tacgtcacat ggattttggg cacgccggaa gtgtttgtcg cttttctcac cacttattgc
     7081 gtgggtaacc tgattggttc cgcactggca aaacctctga ccgactggaa atgtaaagtc
     7141 actatcttct ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat gttctttgtt
     7201 cccatgcagg ccagcatcac tatgtttgtc ttcatcttcg tgattggtgt gttgcatcaa
     7261 ctggtgacac ctatccagtg ggtaatgatg tccgataccg tcgactacgg cgagtggtgc
     7321 aatggtaaac gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct caaactgggg
     7381 ttggccttcg gcggcgctct tatcggctgg atgctggctt atggcggata tgatgcggca
     7441 gaaaaagcgc agaacagcgc cacgattagc atcattattg cgctattcac gattgttccg
     7501 gcgatctgtt atttgctgag cgcgattatc gctaaacgct actactcact cacgacgcac
     7561 aatctgaaaa ccgttatgga acagctggct cagggtaaac gccgttgcca gcaacaattc
     7621 acctctcaag aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg
     7681 attcaacctg gcctcaattt gattcacccg cttcaggtgt tcgaggttga acagcaggat
     7741 aatgaaatgg tggtctatgc tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat
     7801 actcctttat ttacgttgcg ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt
     7861 gagcattttc agggggcgct gaataacggt cctcattatc cgctcaatat tttgcaggac
     7921 gtgaaggtca caatcgaaaa cacagaacgt tatgctgagt ttaaaagtgg caacttaagc
     7981 gcgcgtgtca gcaaaggtga gttctggtca ctggattttc tgcgcaacgg cgaacgtatt
     8041 accggtagtc aggtgaaaaa taatggctac gtgcaggaca cgaataatca acgcaattat
     8101 atgtttgagc ggcttgatct tggcgttggc gaaacagttt acggtctggg agagcgcttt
     8161 actgccctgg tgcgcaatgg ccagacggta gagacctgga accgggacgg cggcacaagt
     8221 actgaacagg cgtataaaaa tatcccgttc tacatgacta accgtggtta tggggtactg
     8281 gtcaatcatc cccagtgtgt ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag
     8341 ttcagcgttg agagtgaata tctcgaatac tttgttatcg acggcccgac gccgaaagcg
     8401 gtacttgatc gttatacccg ctttactggt cgtccggcgc tgccgcccgc gtggtccttc
     8461 ggcctgtggc taaccacttc atttaccacc aactacgacg aagcgacggt aaacagcttt
     8521 atcgatggta tggcggaacg caatctgccg ctgcatgttt tccactttga ctgtttctgg
     8581 atgaaagcct tccagtggtg cgattttgag tgggacccgc tgactttccc tgacccggaa
     8641 gggatgatcc gccgcctgaa agcgaaagga ctgaaaatct gcgtctggat taacccctat
     8701 atcggtcaaa aatcccccgt ctttaaagag ttacaagaga aaggctattt actcaaacgc
     8761 ccggacggtt cgctatggca gtgggataaa tggcagccag gtctggcgat ttatgacttt
     8821 accaatccgg atgcctgcaa atggtacgcc gacaaactga aaggtctggt cgcgatgggc
     8881 gttgattgct ttaagaccga ctttggcgaa cgtatcccaa ctgatgttca gtggtttgac
     8941 ggttccgatc cgcagaaaat gcataaccat tatgcgtaca tctacaacga actggtgtgg
     9001 aacgtgctca aggacaccgt tggtgaggaa gaagctgtct tgtttgcccg ctcggcctcc
     9061 gtcggtgcgc agaaattccc ggtacactgg ggtggcgatt gttacgctaa ctacgaatca
     9121 atggcggaaa gcctgcgcgg tggtttgtct attggccttt caggttttgg cttctggagc
     9181 cacgatatcg gcggctttga aaataccgct ccggcgcacg tttacaaacg ctggtgcgcg
     9241 tttggtttgc tctccagcca tagccgttta cacggtagca aatcttatcg tgtgccgtgg
     9301 gcctacgatg atgagtcctg tgatgtggtg cgcttcttca cgcaactgaa atgccgcatg
     9361 atgccgtatc tgtatcgtga agctgcgcgt gcgaacgcgc ggggtacgcc gatgatgcgg
     9421 gccatgatga tggagttccc ggacgatccg gcttgtgatt accttgaccg tcaatacatg
     9481 ttaggcgaca acgtgatggt tgcgccggtg ttcactgaag cgggcgatgt gcagttctac
     9541 ctgccggaag gtcgctggac acacctgtgg cacaacgatg agctcgacgg tagtcgctgg
     9601 cataaacagc agcacagctt cctgagtctg cccatttatg tgcgtgataa caccctactg
     9661 gcgctgggca acaacgatca acgtcccgat tacgtgtggc acgaaggcac ggcattccac
     9721 ctctttaatc tgcaagacgg gcatgaagcc gtctgtgaag tgcccgctac tgacggatcg
     9781 gtgatcttta ctttaaaagc agcacgtact ggcaacacga ttactgtgac tggtgcgggc
     9841 gaggcgaaga actggacgct gtgcctgcgc aatgttgtga aagtaaatgg tctgcaagac
     9901 ggttcgcagg ctgaaagtga gcaggggctg gtggtgaagc ctcaagggaa tgcgctgaca
     9961 attacgttgt aatctattga tggaatgccg ggtacgtctg agtcgtaccc ggcgagattg
    10021 atgtttaccg caccggctcc gtcgatgtaa cttcaccgct ggaaacgatg ccagcattca
    10081 tggtttgagt cacttgctgc tttgcggcgt tcacggcatg caattgcccg ctaaccgtag
    10141 gtttcaacgg gacattggcc tgaatatcgc cactggcggt aagctgaata ttgccgtcgc
    10201 cagtcaacgg taatttaggc catccccact gttgcaaaat attcaccggc acaccgcgtc
    10261 cattcaggct gatatgtgtc tgacgttgtg gcgtttgtga aacactggcg gtggcttcca
    10321 gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcgg
    10381 tcagcgccag cgacgggcga cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca
    10441 gattcgccga gccactccag acgccccatt tatgatcggt aaccagcgtc aggttggcac
    10501 cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat
    10561 tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca
    10621 tccacaactg ttgccagttt ttcggcaagg tatattccag cccggcaatt gccacatcat
    10681 caaggatcaa cgttttcccg tcacgcagcc agttccctga cgttctgacc ataccccctt
    10741 cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa
    10801 tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct agcgttcatc gacagtttgc
    10861 cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt
    10921 cggtcaccgc ccagtccgga ccttgcaaac gagcatcgat cacttccagg cgaccaatct
    10981 gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt tcgctttgta
    11041 gacgtatatc cgccatgcgc aggttttcca cttgccagct gccgtcggcg ttacgctgcg
    11101 cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc gtaacgcgat
    11161 cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg
    11221 atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct tttggtgacc
    11281 acggaactac gccgccattt acccgctgcg cgctcagttt ccattcgcta ttcgggctat
    11341 taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt
    11401 cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt ggttcggtca
    11461 gttgccgact gcttagcgca atgtcgacac tttttgccac cagggtcgcg ggctggccat
    11521 cacggccaaa cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa cggtgatcca
    11581 tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg atatgttctg
    11641 ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga
    11701 gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaatgcacat
    11761 aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga
    11821 ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca
    11881 gattcagcac gatggcagta ataccgcccg cggcgatccc ggaggagagc aggtttttca
    11941 gccattcagg ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga ccaaccgcca
    12001 gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ctcacgagaa acgatacgca
    12061 caccggaggc ggcgatggtg ccaaacatta caagcgttgc gccgcccaga accggttctg
    12121 gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc aacatcagcg
    12181 cgacgacaaa accgacatag cggctggcaa caccagtcaa ctggatcact ccgttgtttt
    12241 gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag ttcaggccgt
    12301 ttgccagcac gccacctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg
    12361 aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca
    12421 gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc ggcgttggca
    12481 ccataatcag ttcttgcgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg
    12541 ccagcgcata tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag taagggttac
    12601 gttgacggtt aagcaggata attaaggcta agaccacgcc tgccagcagc agatttttcg
    12661 gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggac gttaacccaa
    12721 cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc ggcgtaataa
    12781 tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtgcaactt gccagcatca
    12841 acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtt ttcagcgcgg
    12901 taccgcccat aatcagcggg gcaacaaagt tgaagctggt gccctgaata gacaacagcc
    12961 cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca
    13021 gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta
    13081 atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag gcggcaaaca
    13141 gggtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct
    13201 gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg
    13261 tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt gccacatcct
    13321 catctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt atttttctgc
    13381 ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa agcataactc
    13441 gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga gtatgaaaga
    13501 gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa cgcttgttgc
    13561 cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct ttttgaagaa
    13621 atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc tactagtact
    13681 gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc cgttaatgct
    13741 ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg ccggtgggcg
    13801 tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa atgccattgg
    13861 cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg ccggttctat
    13921 tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca ttgaacgtta
    13981 tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc tggtgctggg
    14041 cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca cgccgaacgg
    14101 tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg gccgcatgat
    14161 cacctcgctg gtgctgattg aaactatcgc gctgattgct atctgcctga cggtggggaa
    14221 aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg tctgtgtact
    14281 gtttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc gcgtctttga
    14341 gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat
    14401 ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga ttctggtggt
    14461 acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga tgggcaaaaa
    14521 ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac
    14581 ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca tggcgttttt
    14641 ggtggtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg tgattaagct
    14701 gtatttaatg ttgccgattt ttgccggtta aaaatgaaaa agcgtcggaa ggaaccggaa
    14761 acgctacgtg aacactgtcg ccatattttt ggtgatgagc cgccagtgct ctgtgtatgg
    14821 gaaacggaat ttgattacgc agatgccgaa ctcaaggcgc tggcggctaa agagtggcag
    14881 cagatcagcg tatgggattt gagtgcttac tatgtactca atctcgttta taacgaaccg
    14941 atgcaaatag agttgtttcg ctatctgttt ccgctctgcc tggcgcaatg gcatgaaacc
    15001 gttttagctg gcggttatgg tgatcacttc gaagaatcct taatgaaggc attgtgtcgg
    15061 ccctatctct ggcaggagat gatgaatgct tcccaacgcc agcaggtacg gcaattttta
    15121 ctggacaccg cgttgcagcg tatggataac gagcgtggtt tcaataacgt tctttgctgg
    15181 ctggcggtgt tcaatactct gggtggggcc gcgccgttaa ttcattctct ctggtcgcgc
    15241 tggtgggcgc tggatacgcc aggaaaagcg gtgtgtgcta ttcagtatgc cgcgcatcta
    15301 atttatccca tagaagccaa tccgttatgg tcgcaagaat ggataggctg ggggcatcca
    15361 ttaggccata aagatggctg gtcctctgat aatcgcgctt ttcttcgcca aatgttaaca
    15421 cctgaaatga ttgtggctgg cgtacaggca gcggctgaaa ttctgcgtgg tgaaccggaa
    15481 ggcgcaatgg cggcgcgaat tgcacaggat gcgtatgagg caatggatat cctcactatc
    15541 cagattgaag atttactgcg tgacttgtct tgtgatgagt ctggtcatgc gttggagtaa
    15601 cgttccgtct ccggcatcca gcgttctatc agggcttttg cttgttgtgg gtaacgttcg
    15661 tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc
    15721 gccactttaa actcagcatt acccgtctga cgcgtaccta acagttcacc cggtccgcga
    15781 atctctaagt ctttctgtgc aatcacaaaa ccgtcgttgc tgtcgcgcag cacttgcagc
    15841 cgaatttgcg cggttttgga gagcggcgtt ttgtagagca gcacgcagtg agaagccacc
    15901 gcaccacgac ctacacgccc gcgaagctgg tgtaactgcg ccagacccag acgctccggg
    15961 ttttcgataa tcatcagact ggcgttaggc acatcaacgc cgacttcaat aacggttgtg
    16021 gcaaccagca ggtgtagctc accttgttta aacgacgcca tcaccgcctg tttctcgaca
    16081 ggtttcatcc gcccgtgtac caggccaacg ttcaactctg gtagcgccag tttcaactct
    16141 tcccaggtag cttccgccgc ctgcgcttcc agcaattccg actcttcaat caacgtacaa
    16201 acccagtatg cctgacgacc ttcagttatg caggcgtggc gcacgcggtc aatgatgtcg
    16261 gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg cggcagctca
    16321 tctatcaccg aggtatcgag atcggcatac gcagtcattg ccagcgtgcg ggggatcggc
    16381 gtggcggtca tgatcaactg atgcggatgg aagccctgct gctggccttt ctcccacaat
    16441 gccagacgct gatgcacgcc aaaacgatgc tgttcgtcga taatcaccag cgccaggccg
    16501 ttaaactgca cctgttcctg gaagatggcg tgtgtaccga caatcatctg cacctgaccg
    16561 ctggcgatgg cttcctgctg tgccagccgt gctttacctt tctgcttacc ggcgagccag
    16621 cccacttcga taccgagcgg tgcaaaccag ttgcggaagt tattggcgtg ctgctcggcg
    16681 agtaattcgg ttggtgccat cagggccacc tgtttgccgt gggcaatcgc acgcagcgcg
    16741 gcgagggcgg cgaccagtgt tttaccggaa cctacatcgc cctgcaccag gcgcatcatt
    16801 ggaacatcca gcgccatatc atgctcgatc tccgccacta cgcgtgcctg tgcgcccgtt
    16861 ggcttgaacg gtaaggcggc gaggagttta tttttcagcg cgtcattggc gctcagcggc
    16921 tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt gtgcgccagc
    16981 agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag atcgctaagc
    17041 tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct
    17101 tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag atccagcgcc
    17161 tggtctgtta atttacgcag cgtagcctgc tttacgcctt ccgtagttgg gtaaaccggc
    17221 gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta ttccgggtgg
    17281 atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac acggcggccc
    17341 gtcgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt gaggatgccg
    17401 gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat attgcagttc
    17461 agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat ggggtagaga
    17521 tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac ggtatgcagg
    17581 ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga actgagtggg
    17641 acagcatcta acaggcgacc tttcatggcc cttaccctgc agcctgcata gtagcccacc
    17701 agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc aggcctttgc
    17761 gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt tgttgctctg
    17821 cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc tgacgctggg
    17881 cttcgtaaag aatgagggct gaggcaacgg aaacattcag cgactgcacc atgccgatca
    17941 tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg cccgttttct
    18001 cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt
    18061 tatcagaaag atgggttgcc agaatctgca tgccctggcc tttcagatga gcgacggcat
    18121 cgccaatggt gcggtgtgtt ttcacctgca cccagctgtt actacccgca gctgccgaag
    18181 ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg ccaacggcat
    18241 ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc atgcagacgg
    18301 tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg agcataacgt gttgggttca
    18361 taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg cggattttgc
    18421 gcatgatatt cgccagatgc acacggtcac gagcggtcag acgaataaag gcgctgtaga
    18481 cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaggtcgtg gtgttaattg
    18541 ccgccgtcag gtttgccagc gcaccctgat gattgaacat ctccaccttg atttcggtga
    18601 tgaattcctg cgccgtctct ttatcccatt ccacagccat aaacttctct ggctctttct
    18661 ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta ccggggctga
    18721 cgtgggcgat aatcgggtcg ccaggaatag ggcggcagca tttcgcaaag gtgatcagca
    18781 cgccatcggc acctttaatt ggcagatgtc cgtgactttg ggttgccggt ggaatggagg
    18841 cgtccccatg ttgtagattt ttcgcgacca ccacgctcat tgcgttacca aggccgattt
    18901 cagccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc tgaatatttt
    18961 cctgcgggat ttcattcagc ttacggctac cacccaaagc atggttgagc agacgacggc
    19021 ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt ttggcgcgcg
    19081 cttttgagct aacgacaaag ttcagccacg cggcattcgg gcgagcgccc ggtgcggtaa
    19141 tgatttcaac ggtttgaccg ctggtaagcg gctgcgacag cgggtaaggc tggcggtcaa
    19201 cgcgtgcgcc cacgcaggca tgaccgatat cggtatgcac tgcataagcg aagtcgacgg
    19261 gcgttgcacc ggcaggcagc tcgacaatgc gcccttccgg tgtgaaaacg taaatctcat
    19321 ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg gcgctctgtt
    19381 gcagctccag caggctttgc atccagcgct gggcgcggat ttgtgcggta gtactggttt
    19441 cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc tgatccatat
    19501 cttcggtacg gatctggacc tcaaccggca cgccgtgcgg gccaatcatc gaggtgtgca
    19561 aagactgata gccgttcgct tttggaatgg cgatatagtc tttcacgcgg cccggacgcg
    19621 gcttgtacag gctgtgcatc tggcccagca cgcgataaca ggtgtcagaa tcattgacga
    19681 tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg agcaccattt
    19741 tgcagtaaat cgaataaaga tgcttttcgc gaccactgac gcggcacggt attcccgctt
    19801 cctgcaaacg cccttcgatt tcagagagga ttttttggat catctcttta cggttgccgc
    19861 gcgcggcttt caccacttct ttaattacgc ggtaacgatt gggatacagc gcctcaaaac
    19921 ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc agcgggctat
    19981 aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg
    20041 tgcgcatgtt gtgggtacgg tcggcaagtt tgatgaggat gacgcggata tcctgcacca
    20101 tcgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatcg cggaacttga
    20161 gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca aaaagctgtt
    20221 ccatatcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc gccgccatca
    20281 gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc gggtgcgtga
    20341 tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg
    20401 cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc
    20461 tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca gcaatagcgg
    20521 taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga
    20581 ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca gtggttttat
    20641 cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga gcgcgacgcg
    20701 cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc tgaacagtta
    20761 cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga tactgaaatc
    20821 aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt tggcggctca
    20881 tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta tcgaagtcat
    20941 cattcacaat cagataatca tattcggcgt aatggctcat ttctgcaaca gcttgcgcca
    21001 tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt
    21061 caattttgga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc tggcgaattt
    21121 gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc agtacttgct
    21181 caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg
    21241 catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa tgttcaccgt
    21301 ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca
    21361 acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca ctgggggcag
    21421 aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta gcgaaataaa
    21481 gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt gctcacactt
    21541 tttgcgcaaa gttgttgcgt tttgcgtagc tgtttccgca tttgcctgtg atttactgca
    21601 ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc ggctttgcct
    21661 tcaccacgtt gtatatctgc cagcctgact acagcggagg tgaagatgaa agtatggatg
    21721 gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg tccggcctgg
    21781 tcgccagcca gagcacagga agaaatttcc cgcctgcaac agcaaataaa acagtgggac
    21841 gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga tcagttaagc
    21901 gcccgtctta cgcagtggca acgctgcttt gtgagcgagc cccgcgacgt gatgatgccg
    21961 cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa aatggcggat
    22021 aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca gccaaaagtt
    22081 gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc aatcagtcgc
    22141 ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc cgctgtgccg
    22201 caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt tctccagcgc
    22261 gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt tgctggcctg
    22321 atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt ctgggcatgg
    22381 ccggatggac cgcagttaat gaccgatcgt ttaaaggagc tggctaccgc aggttttact
    22441 ctaacgcaga ggtatacccg tgcggttaaa aatgctgatg aggttgcgcg cgtacgcaat
    22501 gagtggtgga aagcgaaatt acccttcgtc accgatggcg tggttgtgcg aggggcgaaa
    22561 gaacccgaat cccgccattg gctaccaggc caggcagaat ggctggtggc ctggaaatat
    22621 caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg taagagcggt
    22681 aaaatatcgg tggttgcgtc actcgcacct gtcatgctgg atgataaaaa agtccagcgg
    22741 gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg tgatcagatc
    22801 ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtctg gcgcggtgca
    22861 gaacgtacaa aaccgacacc accagaaaac cgctttaact cgttgacctg ctactttgct
    22921 tctgatgttt gtcaggaaca gttcatttca cgcttagtct ggctggggtc aaaacaggtt
    22981 ctggggctgg atggtattgg tgaggccggc tggcgcgcgc tgcatcagac tcatcgcttt
    23041 gagcatatct tttcctggct tttattaacg ccagagcaat tacagaacac gccggggatc
    23101 gcgaaaagta aaagtgcgca gctatggcat cagtttaatc tggctcgtaa gcagcctttt
    23161 actcgctggg tgatggcaat gggaataccg ctaacccggg cggcgcttaa tgccagtgat
    23221 gaacggtcct ggtcgcaact tttatttagc acggagcagt tctggcagca gctgccgggg
    23281 actggatcgg ggcgcgccag acaggttatt gaatggaagg aaaatgcgca aatcaagaag
    23341 ctgggcagtt ggctggctgc ccagcagatc acaggttttg aaccttagtg tccttcgtgg
    23401 ctgtagtaaa agaccggtaa tcccagctta aggcgtagcg ccagtaagcg ggcaaaaaag
    23461 ccgaatacca gggtggagat aattaccaca tcatggttag aaacatagtg ttgcagcgcg
    23521 atgtacagca cggcggaggc aaatgagaca ccggcataca actctttctg gaataccagt
    23581 gggatacgtt tacagaacat atcgcgcaaa acgccgccaa acacgccggt ggttaccgcc
    23641 gcgacaacgg caataattgg gccgtgcccc atatccagtg caacctgtgc gccgatgata
    23701 gaaaagacca ccagtccaag cgcatcgagc accagaaaca ctttgcgcag gtaaggcatt
    23761 acaggagcaa cgatagtggt gagtactgcg gcggtagcga cgataatcac atattccggg
    23821 tgtttgaccc agccgagcgg atagtggccc agcagaatat cgcgcactga ccctccgcca
    23881 attgcggtgg cggtcgcaat aataattacg ccaaatgtat ccatgcggcg tcgcccggcc
    23941 gccagagccc ccgtcatggc ttcggcagta atgccaacca aatacaaaat gtgtaacagc
    24001 atggttctct ccctaaaaat ctgtctggca gggtagcgat ttgtgcgtat tgtcacgatt
    24061 gagatttttt aaggtgagag gaaattgata acttgtagtt atgtgaaatc gctattttct
    24121 gtagcgtaga tgcattcttc tgacttcttg tctgagaaaa tgcataagaa aaaataggtt
    24181 aaaattgagg ggtggaaaag aaaggtgcaa tgtttagcca ttgtgttcac cgcacctgga
    24241 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
    24301 ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac
    24361 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct acccacgtca
    24421 aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
    24481 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
    24541 ttctgactct ttttcagccc cttcagttgg tggatggctt tctgatctaa tccaccatac
    24601 agcccctggt aaccatgatt ctgaaatatg gcgaaatcag tagctgtcgc taccccagct
    24661 tgctgtgctg cctcaaccaa ttgtttgtta tgttctttca actcatttct cagaaacaga
    24721 cgcttttcgt cttcacgaag ttgcctgaaa gcttcatcgt cagcaagctc ctgccgtcgg
    24781 gtctggatag caaaataagt ctgccctgcc gcaatgaccg gtttcgcagg gtcgccgttt
    24841 tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc tcgttgagcc
    24901 cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc agcttgattg
    24961 ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga
    25021 taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc agtgccataa
    25081 tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
    25141 ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc tcacgttctc
    25201 agtatgctgg gcaaaaaaac atcgcaaaat ctggagcagg cacgcaaaac agaattgaag
    25261 ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag gctgaaaagt
    25321 aagaattagt gttacgagtt actcaatatt ctgaatctgc tcgcgcatct gctcaatcaa
    25381 cactttcagc tcaatcgcgg agtttgtcac ttcggcattg atagacttcg acgcaagagt
    25441 gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa cggcttcttt
    25501 tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca gttcttcggc
    25561 aacatcaatt cgttgtgcca gcagaaccaa ctcctgctcc aggcggttgt tttccagttg
    25621 cacttgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca ggatttccgg
    25681 catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct gctcgatcaa
    25741 tgctttcagt gcctgacctt cggtttcgcg cgcgacaata aagtcgtcca gcgtaccatc
    25801 cagcgccgcg agaatttcag cggcaatggc gtcaagatcc tgctcttggg ctgccatcac
    25861 gcccggccag cgtagaatat caaccgggtt gatttcccct tcgtcactct gcatttttac
    25921 ccagttcgcg gcagttacca gctgtttagc cagtttttcg ttgaggatca gctcaccttg
    25981 cgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg tcaggcgaga
    26041 acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca gacgaaagta
    26101 agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc cccattcacc
    26161 cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc ctgtttataa
    26221 aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat aacctccgga
    26281 agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat gcgtccagca
    26341 ggccgtagca ataatcaggt gcgtcccatt accctgactc gtaactatac aaaacatgca
    26401 gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc ctctattgaa
    26461 gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc agagtacggc
    26521 atgctgccac gttctaccca cacccgtaac gctcgtgaag cggcgaaagg taagcagggt
    26581 ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg
    26641 aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc tgatggtggc
    26701 acgcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcact acagaagctg
    26761 gtggaaaacg gcaagctgaa aaccaacccg atgaaaggga tggtagccgc agtttctgtc
    26821 ggaattgtga acggcgaagc ggtttgcgat ctggaatacg ttgaagactc tgccgcagag
    26881 accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca ggggacggca
    26941 gaaggcgagc cgttcaccca tgaagagcta ctcaccttgt tggctctggc ccgaggggga
    27001 atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt aaggcgactg
    27061 atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat
    27121 atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta
    27181 cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc
    27241 gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt
    27301 tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact accgccgtgg
    27361 cgctggcgga gcatcatgac ctggacctgc cgtactgctt taaccgcaaa gaggcaaaaa
    27421 accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta atgctggtag
    27481 atgatgtgat caccgccgga acggcgattc gtgagtcgat ggaaattatt caggccaatg
    27541 gcgcgacgct tgctggcgtg ttgatttcgc tcgatcgtca ggaacggggg cgcagcgaga
    27601 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc
    27661 tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg
    27721 ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa aagtcatccg
    27781 gcgtcatatt actgcaactg cgcggcaatt agcggccagc gggcgtcaaa atcatccgtc
    27841 gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca gaaggccagg
    27901 atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg cattctcttt
    27961 tcacgcaata cctggcgcag ctgcgcttca atacgctcga acagctggtt gatgcgccct
    28021 tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt caggccagga
    28081 ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct
    28141 ttctcatctt tcagaatcag gttgatgcga gtaatcaggc tatcttcgat aaactcaatc
    28201 aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc ttcggaaacg
    28261 ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct ggattccagc
    28321 atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt ttgtttttct
    28381 gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca cgctatgacc
    28441 gcaaacgaaa tgtttgcggc tatgttatga cgttattcgg atgcgtatgt gttactgacg
    28501 accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt
    28561 aaattcagcc tgtactaccg gaacaaaaat catctgggcg atgcgttcgc caggttgaat
    28621 ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct gatagtcaga
    28681 atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca atccggagcg
    28741 cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc cggtcggaac
    28801 cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag
    28861 tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa cgcgcggatc
    28921 cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg agtaataatt
    28981 ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc cagaaaaggt
    29041 gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga
    29101 tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca ttatttgttt
    29161 cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg cctgcgacga
    29221 tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc tgctttttga
    29281 ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcaa ccgataaaaa
    29341 tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgca gtcatcacat
    29401 caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt
    29461 tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg gagctgtgat
    29521 tagagatata acgcaccgga tcgagcggtt cacgcgtcgg accggcggta atcataatgt
    29581 tcagatgttt caggtcgttg acgggcgaaa aatgcgccac cgccatatcc acaatggtta
    29641 acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca ctgtctggcc
    29701 cccagatgag caaaccacgg gaagcaagca cctctaaatt atgctgcgtg gcagcggcac
    29761 ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta gccagacaaa
    29821 tcgtcgatac caggtcattc gccattccgg cagcaatacg ggcaatcaaa tctgccgtgg
    29881 caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc atagcggctt
    29941 cggctgccgg gtccagcaga ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa
    30001 gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg gccccgcgat
    30061 cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg ctaacgccga
    30121 gaacgatttt tttaccggcc aggctcatga tgatttttcc tgtagggtga caccagaagt
    30181 tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagattca ctttgcgagg
    30241 cgctttccag gattgaaaac tggccgtcga tttaacgaaa cggctatgac aggatgcgag
    30301 caccacaaag gaggtgaagg tgaaaaacaa ttcacagctg ttgatgccgc gcgaaaaaat
    30361 gctgaagttt ggtattagcg ccttaacgga tgtcgagctg ctggcgctat ttctgcgtac
    30421 cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga atttcggctc
    30481 tctttatggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
    30541 ggcgaaattt gcccagttaa aggggattgc tgaactggcg cgacgttact acaacgtgcg
    30601 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
    30661 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
    30721 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
    30781 aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
    30841 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
    30901 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
    30961 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
    31021 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
    31081 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
    31141 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
    31201 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
    31261 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
    31321 ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
    31381 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
    31441 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
    31501 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
    31561 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
    31621 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
    31681 ctgcttgccc ccatattgac tgcatctgtt cattcctgga gatgctatgc ctgaattacc
    31741 cgaagttgaa accagccgcc gcggcataga accgcatctc gttggtgcaa ccattcttca
    31801 tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
    31861 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
    31921 gggctggatt atcattcatt tagggatgtc tggcagcctg cgcatccttc cagaagaact
    31981 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggtaaag tgctgcgcta
    32041 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcataatgt
    32101 gctggcccat cttggaccgg agccgctcag cgacgatttc aatggtgagt atctgcatca
    32161 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
    32221 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
    32281 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
    32341 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
    32401 taaaccgggc tatttcgcac aggaattgca ggtttacggg cgaaaaggcg agccgtgtcg
    32461 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
    32521 gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata aacgctacgc
    32581 taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
    32641 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
    32701 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
    32761 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
    32821 ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca gatgcgcggt
    32881 tgcctgctgt gccagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
    32941 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
    33001 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
    33061 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
    33121 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
    33181 cggtgagtaa agaggacacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
    33241 cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca
    33301 ataccggaat agcgtgtgcg gcagcttcca gcggattatg cccaccacgt tcaaccagtg
    33361 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
    33421 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
    33481 ttagtccagc ctggcggaca agattaatcg catccgggaa gcgttctgga tgacggggta
    33541 ccaggatgag caataaattc gggaattgct gtaacaatgc ctgatgtgcg gcgatcacca
    33601 cactctcttc gccttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
    33661 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
    33721 ggctaccggt aacggtcacc tgattatttt ttgcgcccag cgccacaaaa cgtgcaccat
    33781 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag caagcgacgg acgaatttac
    33841 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
    33901 gaattttacg tttgtgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
    33961 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
    34021 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
    34081 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
    34141 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
    34201 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
    34261 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
    34321 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
    34381 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
    34441 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
    34501 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
    34561 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
    34621 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
    34681 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
    34741 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
    34801 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
    34861 aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta
    34921 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
    34981 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
    35041 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
    35101 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg
    35161 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
    35221 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
    35281 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
    35341 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
    35401 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
    35461 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
    35521 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcacatattg
    35581 ccgctgcagt taatacgccg ctgatatcgc tgtttggcgc aacagatcat attttctggc
    35641 gtccctggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
    35701 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
    35761 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
    35821 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
    35881 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
    35941 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
    36001 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
    36061 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
    36121 gaaagtagcg caggaaaaag gctttttcta tcgcctgacg tcacgttatc gccattatgc
    36181 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
    36241 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
    36301 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagccaata gccgtgaaat
    36361 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
    36421 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
    36481 gcgccacaac acattgctat atgttgttgg gcaggataaa ccgcgaaaat ttgaggcact
    36541 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
    36601 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cggcgggaat
    36661 tgtgctgctg gaagcgatta ctgcaggatt accggtacta acaactgccg tttgtggcta
    36721 tgcgcattat attgtcgacg ctaattgcgg cgaggctatt gctgagccat tccgccagga
    36781 aacattgaat gagattttac gcaaagcgtt aacacaatct tcattgcgcc aggcttgggc
    36841 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
    36901 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
    36961 gcggcaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggtattt cgtgaactgg
    37021 aaactcgccg tactctgcgc tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
    37081 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
    37141 gcgctgaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
    37201 atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca tttattatta
    37261 ccgaagatct gacaccaacc ataagtctgg aagattactg tgctgactgg gcgactaacc
    37321 ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
    37381 tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
    37441 ctttttccgg taaggaagag gagttaaaaa tttcagtaat tgacctgcac cgggcgcagc
    37501 ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt
    37561 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg
    37621 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
    37681 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact tatttttgcc
    37741 aaaattttgg atacagaata aatatgtctc aactcaatga tagtgacatc atcctttttg
    37801 agtataattt tcattatcaa aatataagat ctaaaaatac tcttgatata gcatttggta
    37861 ttgacagaaa ttttcttttt ggatgtggtg tagccatcgc atctattcta ttaaacaata
    37921 gagaaatctc ttgtgaattt ca
//
LOCUS       DAZFFI010000036        37934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFFI010000036 DAZFFI010000000
VERSION     DAZFFI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.036"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..388)
                     /gene="yebO"
                     /locus_tag="ACHJ12_002141"
     CDS             complement(101..388)
                     /gene="yebO"
                     /locus_tag="ACHJ12_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4989920.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(463..606)
                     /gene="mgrB"
                     /locus_tag="ACHJ12_002142"
     CDS             complement(463..606)
                     /gene="mgrB"
                     /locus_tag="ACHJ12_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4989921.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            765..1004
                     /gene="yobH"
                     /locus_tag="ACHJ12_002143"
     CDS             765..1004
                     /gene="yobH"
                     /locus_tag="ACHJ12_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4989922.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1147..1938)
                     /gene="kdgR"
                     /locus_tag="ACHJ12_002144"
     CDS             complement(1147..1938)
                     /gene="kdgR"
                     /locus_tag="ACHJ12_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4989923.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2115..3488
                     /gene="yebQ"
                     /locus_tag="ACHJ12_002145"
     CDS             2115..3488
                     /gene="yebQ"
                     /locus_tag="ACHJ12_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4989924.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3534..4415)
                     /gene="htpX"
                     /locus_tag="ACHJ12_002146"
     CDS             complement(3534..4415)
                     /gene="htpX"
                     /locus_tag="ACHJ12_002146"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4989925.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4607..6655)
                     /gene="prc"
                     /locus_tag="ACHJ12_002147"
     CDS             complement(4607..6655)
                     /gene="prc"
                     /locus_tag="ACHJ12_002147"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4989926.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVILT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6675..7373)
                     /gene="proQ"
                     /locus_tag="ACHJ12_002148"
     CDS             complement(6675..7373)
                     /gene="proQ"
                     /locus_tag="ACHJ12_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4989927.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7470..7967)
                     /gene="msrC"
                     /locus_tag="ACHJ12_002149"
     CDS             complement(7470..7967)
                     /gene="msrC"
                     /locus_tag="ACHJ12_002149"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4989928.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8097..9380
                     /gene="yebS"
                     /locus_tag="ACHJ12_002150"
     CDS             8097..9380
                     /gene="yebS"
                     /locus_tag="ACHJ12_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4989929.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9349..11982
                     /gene="yebT"
                     /locus_tag="ACHJ12_002151"
     CDS             9349..11982
                     /gene="yebT"
                     /locus_tag="ACHJ12_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4989930.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12062..13501
                     /gene="rsmF"
                     /locus_tag="ACHJ12_002152"
     CDS             12062..13501
                     /gene="rsmF"
                     /locus_tag="ACHJ12_002152"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4989931.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13619..13855
                     /gene="yebV"
                     /locus_tag="ACHJ12_002153"
     CDS             13619..13855
                     /gene="yebV"
                     /locus_tag="ACHJ12_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4989932.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13960..14151
                     /gene="yebW"
                     /locus_tag="ACHJ12_002154"
     CDS             13960..14151
                     /gene="yebW"
                     /locus_tag="ACHJ12_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4989933.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14152..14808)
                     /gene="pphA"
                     /locus_tag="ACHJ12_002155"
     CDS             complement(14152..14808)
                     /gene="pphA"
                     /locus_tag="ACHJ12_002155"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4989934.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15204..15545)
                     /gene="yebY"
                     /locus_tag="ACHJ12_002156"
     CDS             complement(15204..15545)
                     /gene="yebY"
                     /locus_tag="ACHJ12_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4989935.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15558..16430)
                     /gene="copD"
                     /locus_tag="ACHJ12_002157"
     CDS             complement(15558..16430)
                     /gene="copD"
                     /locus_tag="ACHJ12_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4989936.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16434..16808)
                     /gene="yobA"
                     /locus_tag="ACHJ12_002158"
     CDS             complement(16434..16808)
                     /gene="yobA"
                     /locus_tag="ACHJ12_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4989937.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16947..17177
                     /gene="holE"
                     /locus_tag="ACHJ12_002159"
     CDS             16947..17177
                     /gene="holE"
                     /locus_tag="ACHJ12_002159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4989938.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17279..17935
                     /gene="yobB"
                     /locus_tag="ACHJ12_002160"
     CDS             17279..17935
                     /gene="yobB"
                     /locus_tag="ACHJ12_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4989939.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17959..18621
                     /gene="exoX"
                     /locus_tag="ACHJ12_002161"
     CDS             17959..18621
                     /gene="exoX"
                     /locus_tag="ACHJ12_002161"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4989940.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18618..20678)
                     /gene="ptrB"
                     /locus_tag="ACHJ12_002162"
     CDS             complement(18618..20678)
                     /gene="ptrB"
                     /locus_tag="ACHJ12_002162"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4989941.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHREFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLYDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20887..21546)
                     /gene="yebE"
                     /locus_tag="ACHJ12_002163"
     CDS             complement(20887..21546)
                     /gene="yebE"
                     /locus_tag="ACHJ12_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4989942.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21873..22229)
                     /gene="yebF"
                     /locus_tag="ACHJ12_002164"
     CDS             complement(21873..22229)
                     /gene="yebF"
                     /locus_tag="ACHJ12_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4989943.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22296..22586)
                     /gene="yebG"
                     /locus_tag="ACHJ12_002165"
     CDS             complement(22296..22586)
                     /gene="yebG"
                     /locus_tag="ACHJ12_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4989944.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22720..23898
                     /gene="purT"
                     /locus_tag="ACHJ12_002166"
     CDS             22720..23898
                     /gene="purT"
                     /locus_tag="ACHJ12_002166"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4989945.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23954..24595)
                     /gene="kdgA"
                     /locus_tag="ACHJ12_002167"
                     /gene_synonym="eda"
     CDS             complement(23954..24595)
                     /gene="kdgA"
                     /locus_tag="ACHJ12_002167"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4989946.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24632..26443)
                     /gene="edd"
                     /locus_tag="ACHJ12_002168"
     CDS             complement(24632..26443)
                     /gene="edd"
                     /locus_tag="ACHJ12_002168"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4989947.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26678..28153)
                     /gene="zwf"
                     /locus_tag="ACHJ12_002169"
     CDS             complement(26678..28153)
                     /gene="zwf"
                     /locus_tag="ACHJ12_002169"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4989948.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSREIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28491..29360
                     /gene="hexR"
                     /locus_tag="ACHJ12_002170"
                     /gene_synonym="yebK"
     CDS             28491..29360
                     /gene="hexR"
                     /locus_tag="ACHJ12_002170"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4989949.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29488..30930
                     /gene="pyk"
                     /locus_tag="ACHJ12_002171"
     CDS             29488..30930
                     /gene="pyk"
                     /locus_tag="ACHJ12_002171"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4989950.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31061..32032)
                     /gene="lpxM"
                     /locus_tag="ACHJ12_002172"
                     /gene_synonym="msbB"
     CDS             complement(31061..32032)
                     /gene="lpxM"
                     /locus_tag="ACHJ12_002172"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4989951.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32152..33474)
                     /gene="mepM"
                     /locus_tag="ACHJ12_002173"
     CDS             complement(32152..33474)
                     /gene="mepM"
                     /locus_tag="ACHJ12_002173"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4989952.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33490..34422)
                     /gene="znuA"
                     /locus_tag="ACHJ12_002174"
     CDS             complement(33490..34422)
                     /gene="znuA"
                     /locus_tag="ACHJ12_002174"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4989953.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34501..35256
                     /gene="znuC"
                     /locus_tag="ACHJ12_002175"
     CDS             34501..35256
                     /gene="znuC"
                     /locus_tag="ACHJ12_002175"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4989954.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35253..36038
                     /gene="znuB"
                     /locus_tag="ACHJ12_002176"
     CDS             35253..36038
                     /gene="znuB"
                     /locus_tag="ACHJ12_002176"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4989955.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHJ12_002177"
     CDS             complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHJ12_002177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4989956.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHJ12_002178"
     CDS             complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHJ12_002178"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4989957.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9093 a         9781 c         9348 g         9712 t
ORIGIN      
        1 ccagagtttt aatttgaagc agttgcacca tggcagtgcg tataatcagg atgaattgag
       61 taacccttca acgacggggt tactcaagat tgaggaagga ttatcgttcg gcgaccagac
      121 gaataatatc ttcgtcttca accgattttt gactctcaac ggtcttttta tctgctttct
      181 caggttcgtt tgcttcgcac aaacgacgta acagtgcatt ttgacgtttt tgctgatcca
      241 gcaacgcctc aagcagttca atctgttcgt tagtccgaga actggcacga ttgataaaaa
      301 accacaagat gagcccgata agaagaacca ccaccgatac aaccaaagac gcaatgttca
      361 tcacgcctga atttacaact tcgttcattt caccacctct gagtagaggc gctattctac
      421 cactgctgga gaggaagaaa atctagtgct gaaaaaatga tatcaccacg ggataaactg
      481 gttaatggca caaattccgc tgaaaaattg tacatcctga tcgcacatca tgttgaatac
      541 ctgcgcccaa agcagcaagc aagccaacac cacgacaacc agaacgaccc atcgaaactt
      601 tttcactcca atctccgctt cacctacctt atgtcattaa actagcatgc gttagttaaa
      661 cagcgcctaa ctatgtcgat attcgtgctt tttcggaatg agtagcttgc ttcagcgcat
      721 attgccgcta cgattaagcg aacataaaaa gagaagaggt tgtaatgcga ttcatcattc
      781 gcacagttat gctgatagca ctggtatgga taggattatt actcagcgga tatggcgttt
      841 tgattggaag caaagagaat gccgcaggat taggtttgca atgtacctat ctgactgcca
      901 gaggcaccag cacggtacaa tacttgcaca ctaaaagtgg tttcttgggg ataacagatt
      961 gcccactgtt acgtaaaagc aatatcgtgg tcgataatgg ttaagggtaa taaataacca
     1021 gattctcact catgagtaaa atattcttat agcaagcgcg tttaatataa cgcaccttca
     1081 actttatttt tctcatgcta aataaagaac ctgtctgttt gatattccga caggttctta
     1141 ctctcatcag aacggatagt cgtgataacc catttgggca gaaattttgc gcgctgcggt
     1201 atgcaacatt gcgacatatt cctgtaaacg ctcttcagag aaacgcaacg tcgggaagga
     1261 gatgctcaaa ccggcaatga ccacgccaaa gcgatcaaat accggtaccg caatgcatcg
     1321 cagcccttct tcctgctctt cgttatcttc gccatacccc tgctcgcgca cctggtccag
     1381 aacgggtaat aacgcttctg tactggtgat ggtccgctcg gtactgcgtt tatactccac
     1441 gccctcaaga atttgcttca cttcatcgag atcgcgccat gccagcagta ccttaccaat
     1501 cgcggtgctg tacagcggat tacgacgccc aatccgtgaa tacatgcgca aattgtacat
     1561 agagtcaatt ttgtgaatgt aaacaatact gtcttcgtcc agtgcgccga ggtggatagt
     1621 ttctttggtc aggcgggaga tctcacgcat ctggatatct gcgctacgaa ttaaatcgac
     1681 gttttgtaac gcgcgagcgc ccagttcaaa caatttcagg gtcagggaat atttctccga
     1741 ctccccttcc tgcgccacat aacctaaggt tttcatggtc tgtaaaaagc gataaacggt
     1801 gctttttgac atcatgacgc gctgcgacag ctcggttatc cctatttcgc gctcttcacc
     1861 cagcgcctgc aaaatgccaa aaacttttag cacggaagat acagaatcag gctgtttatc
     1921 cagatctgcg ttagccattt atcacctcat tgcgagtgtt ttataaaaat cagaactgtt
     1981 ttttattata atttcgcacc agggtggtcg caatccatct tttgccggtt agttacaatt
     2041 ctgcgacatc caccgtgaat atcagtgcta gaatcatacc cctgttgatt attcaccaaa
     2101 gatataaaat tcctatgcca aaagttcagg ccgacggcct gccattgccc cagcgatacg
     2161 gtgcgatatt aaccattgtg attggtattt cgatggctgt ccttgacggc gcaatcgcca
     2221 acgtcgccct gccaacaatc gccacggacc ttcatgccac gccagccagt tccatctggg
     2281 tagtgaacgc ctatcaaatc gccattgtca tctccctgct ctcgttttcg tttctgggcg
     2341 atatgtttgg ctatcgacgt atttataaat gcggtctggt cgtttttctg ttgtcttcac
     2401 tgttctgcgc cctttctgat tcgctgcaaa tgctcaccct tgcgcgtgtc atacaaggtt
     2461 tcggcggtgc agcgttgatg agcgttaata ccgcacttat ccgcctgatc tatccacaac
     2521 gttttctggg gagagggatg ggcataaact cgtttattgt tgccgtctct tctgctgccg
     2581 ggccgacaat tgctgcagca atcctctcca tcgcatcctg gaaatggtta tttttaatca
     2641 acgtaccgtt aggtattatc gccctgcttc tggcgatgcg ttttctgcca cccaatggtt
     2701 ctcgcgccag taaaccccgt ttcgacctgc ccagcgccgt gatgaacgcg ttaaccttcg
     2761 gcctgcttat cactgcgttg agtggtttcg ctcaggggca atcgctgacg ttaattgctg
     2821 cggaactggt ggtaatggtt gttgttggta ttttctttat tcgccgccag ctttctcttc
     2881 ccgtaccgct gctaccggtg gatttactgc gtatcccgct gttttcactt tctatttgca
     2941 catctgtttg ctctttctgc gcacaaatgc tggcaatggt ttccctgccc ttttacctgc
     3001 aaaccgtgct cgggcgtagt gaagtcgaaa caggtttact tctgacaccg tggccgttag
     3061 caacgatggt gatggctccg ctggcaggct atttgattga acgcgtacat gcaggattgc
     3121 tgggtgcttt agggttgttc atcatggctg cggggctttt ttccctggtt ctgctgcccg
     3181 cgtcacctgc ggatatcaat attatctggc cgatgatctt atgtggcgct ggatttggct
     3241 tattccagtc acccaataac cacaccatta ttacctccgc tccgcgcgaa cgtagcggtg
     3301 gggccagtgg catgttagga acggctcgtc tactgggtca gagtagtggc gcggcgctgg
     3361 tggcgctgat gctaaatcag ttcggtgata atggtacgca cgtttcgctg atggctgcgg
     3421 ctattctggc agtgattgct gcctgtgtga gtggtttacg tatcactcag ccacgatcca
     3481 gggcataaaa aaagcgcgtc gatcaggacg cgctttttag tatttacttc atattacttc
     3541 aggtattcac ccgtacgcag agcttcaatt cgtttatcca gcggcgggtg agtcatgaac
     3601 aactcactga gcgatttcga cttaccgtta atgcagaaag ccatcatgct ggttgcttct
     3661 tgcggttcat agctggtttt caggcgctgc agcgcggcaa tcattttctc gcgaccaacc
     3721 agttttgccg aaccggcatc agcatggaat tcacgatgac gcgagaacca catggtgata
     3781 atgctcgcca gaataccaaa caccagttcc agaaccgttg caaccgcaaa gtagatcagc
     3841 gggttgccgt tgctctcttc accttcatca cgatttccgc ccataaaacc cgcggcaagc
     3901 tgcgccagaa tacgggaaat aaagataacg aaggtgttca ccacgccctg aatcagcgtc
     3961 atggtaacca tatcaccatt ggcgatgtgg ctgatttcgt gagcaattac cgcctcggct
     4021 tcatccgggc tcatgttctg cagcaaaccg gtgctgacag caaccagaga ggcatcacgg
     4081 cgcgcaccgg ttgcaaaagc gttgatgtcc ggcgcatggt agatagccac ttgcggcata
     4141 gcgatccctg cctgacgagc ctgggttgct acagtattaa ccagccaacg ttccctttcg
     4201 ttacgcggtt gctcgatcac ttccccgcca acagatcgta atgccatcca tttggacatc
     4261 agaagcgaaa cgaaggaacc accaaaaccg aacagcaagg ccatgatcat cagcccctga
     4321 acgctgctcg actgtatccc tgtcaggctc agtaccagcc cgaaaacgac cattacggcc
     4381 aggttcgtta gcaggaagag cgcgattcgc atcataattt tctttttacc tcagtttaac
     4441 aaaacgcaat atgcgatacc cacatcgtat gggttacgcg actattttca agtctggata
     4501 gtgcgtaagt caccagaaag acacaacttt acattttgta gcatctgatt tacggcatct
     4561 tgccgctgtt aaaaaatcag gcacaatttc ttgtgcctga ttgatattac ttgacgggag
     4621 cgggttgttc cgcgggtctg gctttttcaa gcttcgccag atcgagtgcg atattcaccg
     4681 tctcatccag ataaggatcc ggctcctggt aatcttttgg tagatcatcc agcttcttca
     4741 actccggttt accttcgcgt ttaaagcgtt cgttcaaacg cgccagacgc gtcgcatcat
     4801 cttcattatt ctctttctca cgcacagcgt aattcagaga aacgatattg cgcttgtcct
     4861 tcatagcgtt gaagcgcgcg atatccttca tgatgttctg gaactcagga tctttcgcga
     4921 tacgcgcatt atgttccttc agtagctccg gttcaaaggc cgttaaatcg cctgatttca
     4981 cataagtcgc agcatcaatg ctatcccacg gcagcgcgtt atcttcgaat ttctcacccg
     5041 tttccgtttc ttcattaccc gtcggcatga tgatgtctgg cgttacgcct ttacgttgcg
     5101 tactgccgcc gttaacgcga tagaatttct ggatcgtgta ctgcacagaa cccagcgctg
     5161 gccattcagg acgtaacatc tgatcgtaaa tacggttcaa tgaacggtat tgctgaacgg
     5221 tgcctttacc aaacgtcggt tcacccacaa ccagcgcacg accgtaatcc tgcattgccg
     5281 cggcaaagat ttctgaagcc gaagcactga aacggtcaac cagtaccacc agcgggcctt
     5341 tatagaacac ctgcccgtcg gtatcgctgt cttcacgaac cttgccgttg ttatcgcgga
     5401 cctgaacaat gggacccgca ggaataaaca gacctgagag cgataccgct tcggtcagcg
     5461 ccccaccgcc attgctacgc aggtcgatga tcacactgct gacattctgt ttttccagtt
     5521 tctgcagttg aactttgaca tcgtctgtca aacccacata gaagcccgga atatccagca
     5581 cgccgacttt ctctttaccg acggtcttca ccgacatttt aaccgcgcgg tcttcgagac
     5641 gaatacgttc acgggtcaat attacagtgc gggtcttggt ccctttacca gcaggtaaaa
     5701 tttccagacg aactttactg cccttcggcc ctttaattaa ggcaaccaca tcatcaagac
     5761 gccagccaat cacgtcaacc atcggcttgc ctgtttgacc aacaccgaca attttgtcac
     5821 caacgctgat agctttactc ttcgctgccg gaccacctgc caccatcgaa ttgataacgg
     5881 tgtagtcatc atccatttgc agcactgcgc caataccttc cagcgacaaa ctcatttcag
     5941 tgttgaactg ttcggtatta cgcggggaaa gatagttggt atgcgggtcg atttcacgcg
     6001 caaacgccgt cattgccagc gagaaaacat cttcgctgtt ggtttgcgcc agacgacgaa
     6061 tggcaaattt gtagcggcgt gtcagggttt cacgaatttc tttatccgtt tttcctgcca
     6121 gcttcaggct taactcgtcg aatttgactt tactgtccca cagcgcgttc aactcagcct
     6181 cgtttttcgg ccagggcgct ttgctgcggt caaggttata agtgtcgttg ccggtgaaat
     6241 ccatcggctt ttccagtacc gacaaagcgt actggtaacg ctcaaaacgg cgcttttgcg
     6301 ccagattgta gagatcgtag aaaacgtcga gtttgcctga acgcagttca tcgcctaact
     6361 cggttttctt tttcgcgaac tgttcaacat cgcttgccag cagcacgttg tggctgtaat
     6421 cgagcagatt caggtagcgg tcaaagattt tggccgaaaa tgcctgatcg aggtcgaact
     6481 ggcgataatg agaacgggtg aagcgcgacg ttacgcgctc acttaccgtc gcatgctgcg
     6541 tctcttcctt taataccgga atttgatcag cacgcgtgat atcttctaca gcgaaggtct
     6601 ggcctgctat tgcaagcagg ccagctaacg cggtaagcct aaaaaacatg ttcatgcctg
     6661 gcccggcctc cgtttcagaa caccaggtgt tctgcgcgca caatcaaaga catacccgaa
     6721 ttcagctgga cgcggacgcc gtctttggtg atttctaata cggtggcatc catcgcgttt
     6781 tgacccgctt tcaccttcag ggcttgtccg acagtcagag ctgaaatgtc agaaaccggg
     6841 gtgtgctgtt cttcgcgagg tgcttttact gtttttggcg ctttctctac cggcttttgc
     6901 gcacgaggtt tacgttcagc gccttctttg cggcgtggcg tggtcggacg tggcttgcgt
     6961 tcgcggcgcg gtgcgtcttc tttctcacca gcagctgcgg cagcttcgcg ttttttcgct
     7021 tgctgttcag cacgctgtgc ctgaacacgc gctttcgctt cttcaagctg cttgcgagca
     7081 tgctctacat gttgctcgtc cagctcaccg catgggttgc cgtcaagatc gacacgcgtt
     7141 gcgccgggtt taacaccgta aagataacgc cagctcgaag tgtagagacg taaagcggat
     7201 cgtaattgcg ttttgctcag gttcatttcc ccggcaacac gatcgaccaa atcctgaaaa
     7261 ataccgattt tcagcggacg cgcttcacct tccgcactga aacagtgggg aaaacgttcg
     7321 gccagaaacg cgattacttc tttactgcta ttcaacttag gttgattttc catgaaattt
     7381 cctgattaca acggacgtag ccaacaagcg caggcatgaa caggcgtcat tataatgacg
     7441 ctaccagtaa atgctacgtt atccgttgat tatcctgcga cgctcgcaaa gaattttttg
     7501 taatccgtcg ttgcaagcac tttttcaagc tgtgccacaa gctgacgtaa gccttgctcg
     7561 tcctcgtctg taaagcgacc gaagacggta ctatcgatgt caagaacacc aataatctga
     7621 tttttcacca ccagcggcag aacaatttca gaattactcg ccgcatcaca ggcaatatgc
     7681 ccgtcaaacg cgtgaacatc ctcgatacgc tgcacttgat tgcgggcaac cgcagtgccg
     7741 cacaccccgc gcccgacggg tatccggaca caggcaattt tgccctgaaa tggtcctagt
     7801 accagtgtat cgtcctcaag caaataaaaa cctgcccagt ttacgtcagt gagacgctcg
     7861 tataacaacg cactggtgtt cgcaagcgtt gccagaaaac tggtttctcc cgccatcagc
     7921 gcgttaaagt cgcgatttaa atccgcgtaa aattctgttt tgttcattat ataatcactt
     7981 agttgtctta cctggaactg ccagcctatt aaaataagca ttaaatgcgt taatgctcaa
     8041 gatcattcca atcatgggtt aatattaatg ttaattctta ttacatttgg cacgtcatgg
     8101 ctcttaacac accacaaatt acgccgacaa aaaagataac agtgagggca atcggcgagg
     8161 aactgccgcg tggtgattac caacgttgcc cgcaatgtga catgctgttt agcctgcccg
     8221 agataaattc tcatcagagt gcctattgtc cgcgctgtca ggcaaaaatt cgcgacgggc
     8281 gcgactggtc gctaacgcgc ctggcggcaa tggctttcac catgctgttg ctgatgccgt
     8341 ttgcctgggg cgaaccgctg ttgcatatct ggctgttagg tattcgtatc gacgccaacg
     8401 tcatgcaagg catctggcaa atgaccaaac agggcgatac gataacgggg gcgatggtct
     8461 ttttctgcgt tattggtgcc cccctcattc tggtgtcctc catagcatat ttatggtttg
     8521 gtaaccgact gggaatgaat ttacgtccgg tactgctgat gcttgagcga cttaaagagt
     8581 gggtaatgct ggatatctac ctggtcggca ttggcgttgc ttctataaag gtacaggatt
     8641 atgcccatat ccaggcgggt gtcggcttgt tctcttttgt ggcgttggtg attttaacga
     8701 cggtgacgtt gtcacatctt aatgtcgagg agctgtggga gcgattttat ccgcagcgcc
     8761 ccgctacgcg tagggacgag aaacttcgtg tctgtcttgg gtgccatttt accggctatc
     8821 ctgatcagcg tggtcgctgc ccgcgttgcc atatcccgct acgcctgcgt cgccgtcata
     8881 gtttgcaaaa atgctgggcg gcgctgttag cgtcaatcgt tttgttgtta cctgccaacc
     8941 tgttgcctat ttctatcatt tatctgaacg gaggacggca ggaagataca attctttccg
     9001 gaattatgtc gctggcaagt agcaacattg cggttgcggg aatcgtgttt atcgccagta
     9061 ttctggtacc gtttactaaa gtgatcgtca tgttcacttt actgttgagc attcatttta
     9121 aatgccagca aggtttacgc acacgcattc tgttactgcg gatggtgacc tggattggtc
     9181 gctggtcgat gctcgacctg tttgtcatat ctttaaccat gtcgctgatt aatcgcgatc
     9241 agatcctcgc ttttactatg ggaccggctg cgttttattt cggcgcagcg gtaattttga
     9301 ctattcttgc tgtggaatgg ctggacagcc gcttactttg ggatgcacat gagtcaggaa
     9361 acgcccgctt cgacgactga agcgcagatt aaaaataaac gccgtatctc acctttctgg
     9421 ctgctgccgt tcatcgcgct aatgattgcc ggttggctga tttgggacag ttatcaggac
     9481 cggggtaata ccgtcaccat cgactttatg tcggcggatg gtattgtccc gggccgtacg
     9541 cctgttcgtt atcagggcgt tgaagtcgga acagtgcagg atatcagcct cagcgacgat
     9601 cttcgtaaga ttgaagtcaa ggtcagcatc aagtccgata tgaaagatgc gctgcgcgaa
     9661 gagactcagt tctggctggt gacgccaaaa gcatcgttgg caggtgtctc cgggctggac
     9721 gccctcgtcg gtggtaacta tatcggcatg atgccaggta aaggtaaaga gcaggatcac
     9781 tttgtcgcac tcgataccca accgaaatat cgactggaca atggcgatct gatgatccac
     9841 ctgcaagccc ccgatctcgg ttcgctgaac agcggttcat tggtctattt ccgcaagatc
     9901 cctgtgggaa aagtctacga ctatgccatc aatcccaaca agcaaggcgt ggtgattgat
     9961 gtcctgatcg agcggcgttt taccgatctg gtgaaaaaag gtagccgttt ctggaacgtt
    10021 tccggcgttg atgccaatgt cagtatcagt ggcgcgaagg tgaaactgga aagtctggcg
    10081 gcactggtta acggtgcgat tgccttcgat tcacctgaag agtcgaaacc tgccgaggcg
    10141 gaagatacct ttggtctgta tgaagatctg gcccacagcc agcgtggcgt aataataaaa
    10201 ctggaactgc cgagtggggc aggattaacc gccgactcga cgccattaat gtatcaggga
    10261 ctggaagtcg gacagctaac taaactggat ttaaatcctg gtggtaaagt cactggggaa
    10321 atgacagttg atcccagcgt cgttaccctg cttcgtgaaa atacccgcat cgaattacgt
    10381 aatccgaaat tatcccttag cgatgccaat ctcagcgccc tgctgactgg caaaaccttc
    10441 gagttggtac ccggcgatgg cgagccacgc aaagagttcg ttgttgtgcc aggcgaaaaa
    10501 gcactgctgc atgaacctga tgttctgacg ctgaccctga ccgcgccgga aagttacggt
    10561 attgatgctg gtcagccgct cattcttcac ggcgtgcagg taggccaggt tatcgatcgt
    10621 aaactcacca gcaaaggcgt cacctttacc gtcgccatcg agcctcagca tcgagaactg
    10681 gtaaaaggcg atagcaaatt tgtcgtcaac agccgtgtcg acgtgaaggt ggggctggat
    10741 ggcgttgagt ttctcggtgc cagcgcctca gaatggatta acggcgggat acgtattctg
    10801 ccgggcgata aaggcgagat gaaagccagc tatccactgt atgccaatct ggaaaaagcg
    10861 ctggagaaca gccttagcga tttacccacc acaaccgtga gtttgagtgc agagacgctg
    10921 ccggatgtgc aggcaggatc ggtagtgctg taccgtaaat ttgaagttgg tgaagttatt
    10981 accgtgcgtc cgcgagctaa cgcgtttgat atcgatctgc atattaagcc ggagtatcgc
    11041 aaccttctga ccagcaatag cgtgttctgg gcagaaggcg gggcgaaagt tcagctgaat
    11101 ggtagtggcc tgaccgtaca ggcatccccg ctctccagag cattaaaagg ggctattagc
    11161 ttcgataacc tcagtggtgc cagcgccagt cagcgtaaag gcgacaaacg tattctgtat
    11221 gcttccgaaa cagcggcccg tgcggttggt gggcagatta cgcttcacgc tttcgatgcc
    11281 ggaaaactgg cggtcgggat gccaattcgc tatctcggta ttgatatcgg gcaaatccag
    11341 acgctggatc tgattaccgc gcgcaatgaa gtacaggcaa aggcggtgct ctatccggaa
    11401 tatgtccaga cctttgctcg cggtggtacg cgcttctcag tggtcacacc gcaaatttca
    11461 gccgcgggcg ttgagcatct tgatactatc ctccagccgt atatcaacgt cgaaccaggc
    11521 cggggcaatc ctcgccgcga ctttgaatta caagaggcca ccattactga ttcgcgttac
    11581 ctggatggct taagcattat tgttgaagcg ccggaagccg gttcgttagg tattggtacg
    11641 cctgtgctgt tccgtggtct ggaagtcggt acggttacag gaatgacgct ggggacattg
    11701 tcagatcgcg tgatgattgc gatgcgcatc agtaaacgct atcaacacct ggtgcgtaac
    11761 aattccgtct tctggttggc atcgggttac agtctggact ttggtctgac gggcggcgta
    11821 gtgaaaaccg gcacctttaa ccagtttatc cgtggcggca tcgccttcgc cacgcctccg
    11881 ggtacgccac tggcaccgaa agcccaggaa ggcaaacact tcctgttaca ggaaagtgaa
    11941 ccgaaagagt ggcgtgaatg gggaactgcg cttcccaaat aatgcccact gctccggcgt
    12001 gcctgcgccg gagcgtttat gctaaactgc gcgcctgttt ttttgccagt ggtacatgct
    12061 cgtggcccaa cacaccgttt atttcccgga cgcctttctg acacaaatgc gcgaagcgat
    12121 gccttcgacg ctctcatttg atgattttct tgccgcctgt cagcgcccgt tgcgccgcag
    12181 cattcgcgtt aatacgctga aaatctccgt tgctgatttc ctgcaattaa ccgctcctta
    12241 tggctggacg cttacgccaa ttccgtggtg tgaagaaggt ttctggattg aacgcgacaa
    12301 tgaagatgca ttgccattgg gtagtaccgc cgagcattta agcggcctgt tttatattca
    12361 ggaagccagt tcaatgttgc ccgttgccgc cttgtttgct gacgataatg caccacagcg
    12421 ggtgatggat gtcgcagctg cgccaggctc caaaacgacg caaattgccg cgcggatgaa
    12481 taacgaaggg gcaatccttg ccaatgagtt ttccgccagt cgggtaaaag tgttacatgc
    12541 caatatcagc cgctgtggca tcagtaatgt tgcgctcaca cattttgatg gccgcgtgtt
    12601 tggtgcggca gtgccagaaa tgttcgatgc cattttgctg gacgctccct gctctggcga
    12661 aggcgtggtg cgtaaagatc ccgatgcgct aaaaaactgg tcaccagaaa gcaatcagga
    12721 aatcgcagct acacaacggg agcttatcga cagcgccttt catgcattac gtcctggtgg
    12781 tacgctggtt tactcgacct gtaccttaaa ccaggaagaa aacgaagccg tttgcctgtg
    12841 gctgaaagag acttaccccg acgcagtaga gtttttacca cttggcgatc tcttccctgg
    12901 tgcaaacaaa gcgctgaccg aagaaggctt tttgcatgtt ttcccacaaa tttacgactg
    12961 cgaaggcttc ttcgttgctc gtctgcgtaa aactcaggcg atccccgcct tacccgcccc
    13021 caaatacaaa gtgggcaatt tcccgttcag cccggtgaaa gatcgcgaag ccggacaaat
    13081 tcgtcaggcg gctgcaagtg ttggcttaaa ctgggatgga aacctgcgcc tctggcaacg
    13141 cgacaaagaa ctgtggttgt tcccggtggg cattgaagcc ctgatcggta aagtccgatt
    13201 ttctcgcttg gggattaaac ttgccgaaac gcacaacaaa ggttatcgct ggcagcatga
    13261 agcagttatt gcccttgcca cccccgacaa tgtgaacgct tttgaactga caccgcagga
    13321 agcggaggag tggtatcgcg ggcgcgatgt ttacccgcaa gccgcgccag tggcggatga
    13381 cgtgttggtt actttccagc atcaaccgat tggtttagcc aaacggattg gttcgcgatt
    13441 gaaaaacagc tatccgcgtg aactggtgcg cgatgggaaa ctttttaccg gtaacgcctg
    13501 acagcgcaca aaaaaagcgc actttttgac tggcacattc ggctgcctca actaggctga
    13561 aaaatggtgc gatcggactg gtcgtaccac aatcggcagc taaatggaga gcacgaagat
    13621 gaaaaccagt gtgcgcatag gcgcttttga aatcgacgac ggcgaattac acggtgaatc
    13681 gccaggtgat cgaacgttaa ccattccttg taaatctgac cccgatttat gtatgcaact
    13741 ggatgcctgg gatgctgaaa ccagtatccc tgccctgcta aatggcgaac actctgtcct
    13801 ttaccgtacc cgttacgatc aacaatctga tgcctggatt atgcgtcttg cctgatccaa
    13861 aaagaacccg tcggcatggc gggttatttg tcctggttat tcccccgttg taaaatctct
    13921 cctaaactta acggtacggc accacacttc ggggatgaaa tgttcgcgct ggtacttttt
    13981 gtttgctacc tggatggcgg ttgtgaagat attgttgtgg atgtctacaa cacggaacag
    14041 cagtgtcttt attctatgag cgatcaacgg atccgccatg gcggttgttt tccgattgag
    14101 gattttatag atggtttctg gcgaccagca caggagtatg gtgattttta attattgcaa
    14161 ttgcacaaga gtcagttcgc ccccaaagac agcaccggta tcaatataat gcaggttgcc
    14221 aatatccacg cgatgtcgca acggtgtatg accaaaccag aaatgatcag cacctgtaat
    14281 tccctgccct ttttggcgtt cacctaatcg cgagcggctc cacaagacct gatgcaaatc
    14341 aacgtccttt tgccattcat aaacatcatc tggataatcg gcatgagcaa taacatgttt
    14401 gccggtacga ctgtgtactt caagaataaa gggcaaatgc tgacattttt ccagcgccgt
    14461 tttcgcttgt ttctgttgat tatctgccag cgcaataaac cagtcgccgc cattcatcaa
    14521 ccacaaagac atctgctggg atgccagcgc atccatcgcc atctgttcat gattgcctct
    14581 taccgcacaa acccaatgtt gttccagtaa ctgcagacaa cgtaaacttt gcggcccacg
    14641 atcgataacg tctcctactg agataagtaa atctcgccac ggatcaaaac gacaatgcca
    14701 taatttgcgg cgcaactgct caagacaacc gtgtatatcg ccagaaagcc agatatgtcg
    14761 ccattgatga cccgcaattc tctgataaac gggcgcaggc tgtttcatca atattttcct
    14821 cccgcgctaa agatcacata atcttaacaa gaatgttaaa aaacgctgga ctcagacagt
    14881 agagtgtgtg ttatggttga ctataaagtc agcgaaggaa atgcttctgg cttttaacag
    14941 ataaaaagag accgaacacg attcctgtat tcggtccagg gaaatggctc ttgggagaga
    15001 gccgtgcgct aaaagttggc attaatgcag gcttagttgc cttgcccttt aagaatagat
    15061 gacgacgcca ggttttccag tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc
    15121 agctgaaatg ttaaaaaccg cccgttctgg tgaaagaact gaggcggttt ttttattgga
    15181 aatcaaaagg ctattttagg taattaacag agtttttcag ctcgttctat aaacggtgcc
    15241 agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga aatgggttgg
    15301 gctttagtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg atactgcacg
    15361 agggtacttg ggttaatgac atacaaagca ttacccggtc tgcaagtcag catcacctct
    15421 tcgcgattaa acgcccattt gtctttaccc acttcaaaac gactgacggt aatcacctgc
    15481 ggtgcagcca gcgccgctgc agaactggtg agtaacagaa acgtcagaat actttttttc
    15541 atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc
    15601 actaccgcac ctatcgccca ttcgagttta gtcatccact caaaccaggg tgccgcacga
    15661 tcttcatctt gcctcatgcg cggtacaaga acataacgat tagccagcgc aatgaccacc
    15721 ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt cgtaagcgtg
    15781 gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac gccgatcacc
    15841 gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa tggcctggtg tcgccaacgg
    15901 cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca cgccgccgca
    15961 caaatcaggt gaatcgcgtg attagtctga tggattttcg ccgttacccc ttcattcagt
    16021 gtcgcatgcc cgattcccgc cagcagaata aattgcgcgg tagtgagcat aaacagcaaa
    16081 cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc gagaacaatt
    16141 tgccataacc agacgccacc aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa
    16201 aatacatctg cccatcccgt tcccatcaac ccgccctgaa cggcgagcat tgcagtggca
    16261 ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag
    16321 cgacgaatcg ttaagggggc cagccaggca ccgtacattg cgaagccaaa gaccagcatc
    16381 agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat gatttatttc
    16441 acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca ccgaaacaac atgccagtca
    16501 acgatgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg cttttggtcc
    16561 tgctcatttc gcttcgccgg taatgtttta atattttcgt tttttggccc cgtgattttt
    16621 gcaccactga atcctgtttc aacaccttcc gagaagttta aagtgattgc ctgcggtgca
    16681 gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg
    16741 gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt
    16801 gaagtcatat tatctattcc tttttgtaat aactttttta cagagcataa ccttgtctaa
    16861 tgtctgagtc gaggatcatc aattccggct tgccatcctg gctcactctt agtaactttt
    16921 gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg gatcaaacag
    16981 aaatggataa agtgaatgtc gatttggcgg cggccggggt ggcatttaaa gaacgctaca
    17041 atatgccggt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg cgcagctggt
    17101 ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg
    17161 agcccaaact taaataaaac ttatacagag ttacactttc ttacataacg cctgctaaat
    17221 tatgagtatt ttctaaaccg cacccataat ttgcagtcat tttgaaaagg aagtcattat
    17281 gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca
    17341 ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc agctgttagt
    17401 ttttccatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc ctgccccacc
    17461 cgatctctca ctgttagacc cgctttgtta tgccgcaacg acctggcgaa tgaccatcat
    17521 tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc
    17581 gtggcgaaaa acgcctggga tctaccatca gagtcatggt gcatgtctgg gtcgtcgttc
    17641 cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc cgacctgttc
    17701 actgtttact acagggcaat gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt
    17761 acaatttttt tcacatcagt actcgattgc cgtgctgatg gcaaatgccc gtggtaacag
    17821 tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt
    17881 agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac gctaggcttt
    17941 ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg tttgcaggga
    18001 gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc
    18061 cacctggtgc gccccgatcg tcctattagt ccacaagcga tggcgattca tcgcatcacc
    18121 gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccacacta ttacggtagt
    18181 gaatggtatg tcgcgcataa cgccagcttt gaccgccgcg tactgcctga gatgcccggt
    18241 gagtggattt gcactatgaa actggcccgt cgtttgtggc ctgggatcaa gtacagcaat
    18301 atggcgttat ataaaacacg caagctcaat gtacagacgc cgccgggcct gcatcatcac
    18361 cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta tcgatattat gaacacctcc
    18421 ggctggacgg cagaacagat ggccgatatc accggacgtc cgtcgttgat gacgaccttc
    18481 acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga tccgggctat
    18541 ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt aacactgaaa
    18601 cattatctgg aaaatactta gtccgcaggc gtagcgggta atgttccctg cgccagcgcg
    18661 accagaaaag catattccat cgctacgcct tcgtacgatt taaagcgacc agatttaccg
    18721 ccatggcctg agtccatgtc ggtacagagc aataaaagat ggtcatcggt tttcagctcg
    18781 cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc gtacaaaccg
    18841 gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatatgg gctgtagctt
    18901 ttcatgtact cgtaatattg cggatcctgc ggattccccc actcttcaaa ctcaccagtg
    18961 gtaagaggaa ttgattcatc aagcatcgtt gttacaacat caacaaacgg tacctgggcg
    19021 ataacgccgt ggaataattc cgggcgttga ttaattgcaa cgcccatcaa catgcccccc
    19081 gcactcccgc ccatcgcata acaaagcgaa ggagagccat agcccagttt taacaatgca
    19141 tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt tccgtcttcg
    19201 taccattgtt gccccagctc accaccgccg cgaacatgga caatggcgta gacaaagcca
    19261 cgatctaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc gccgtaagaa
    19321 ccatagccat acaccagcaa cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc
    19381 aacgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc actgcggtaa
    19441 ttcgccgcat caaaaccggg cacttctgtt tgttttaata cacgacgctc accggtatcc
    19501 atatccagtt caaacaaagt gtctggtgtg gtcatggaag aataaccata acgcaatcgc
    19561 gcggtttcag gttctggatt gtaggcaatc caggtcacat aggctggatc atcaaaggca
    19621 ataccaatga cttcccgggt cttgcggtta atttggcgca aactggttaa cccgcgctga
    19681 cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat gatgttttcg
    19741 cgtggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg gtataagcca
    19801 aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat gctggtagtg atcaaggctg
    19861 tattcgtgat ctttgcggcg cggcagaaaa acaaacggct cggcatcggc catttccgcg
    19921 tccagcaggc gaacttcact ggtggtggcg ctggccaaat gaatgactac atagtgcttc
    19981 gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta gatcagttta
    20041 tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataaggcag cagcgtcacc
    20101 ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa gctgggttca
    20161 acgttatcca gcagttccgg gtaccagtta ccagtttcca gattacgaaa acgaatgccg
    20221 tactggcgtc gggaaagaaa atcttctgcc agcgccataa tggtgttatc gggcgtaatc
    20281 gccattccgc ccatcgaata aaactcacga tgagctgcgc gcttattggc atcgagcaat
    20341 gtttcccact catcccactc ttcactgaat gccgattgac gctggtagat agcatattca
    20401 cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg cgcagaaact
    20461 tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt
    20521 gaggccatca cccgatgacc gtaactattt tcttgttgca ggtagtccag gacttctggc
    20581 tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc gccatgaagc
    20641 gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt tctggttgaa
    20701 acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggg agtcattgct
    20761 tagatgatga caggtaatgg cgcggatatc gaatgttatg cagacagaga aaatcagcct
    20821 gttcaaatgg ctgtgcgatt ctggatagcc cgaaatagtc aacttcaggc tatccagaga
    20881 gcggaattat tccgccaaag tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg
    20941 aacatcctgg gggattttca gcgcgtcacc cagtgcattc aggtaactgc gttccataaa
    21001 atggtcaata tcaatagccg cgcaactcag gaaatagatt tccagcgcct cttcttcatt
    21061 gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct gctcaatgag
    21121 tacacgcccc tgctcttcca cgccggcttc acgcaattgc tggtcgattg ccgcacgttc
    21181 tttggcatca atatgaccat cacttttagc ggcaaagacc agcgcaagga tcaaacgttc
    21241 tgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc
    21301 gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc cgccgccaac
    21361 cagtaacgcg ttggtgccat atttcgtaag aagtttacgt gctgatttat ttgcgaccag
    21421 cagcccagcc agtccgccta atgcgcctgg cactaacagt ttgcccaatc cctgatccgc
    21481 agacgaggag gtagaagaac tgctttgccc aagcagggat tgcagttgat ttaaccagtt
    21541 agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc
    21601 agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa
    21661 agaaattcgc gccaggtaag gtatttactg tatatcaggt gataagggta ttttaggtga
    21721 aagtagtttg tcgttttgag tggcattgtt tgatgctgat tgcccgatgc gacgctgttg
    21781 cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat
    21841 ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc
    21901 gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc
    21961 ggtactgacc atttatcatc tttaccctga atgtcctgca aactgaccca agccacggga
    22021 tcggcctgac cgacaatttt ttgatcatct gcccagcgcg ccacgcgatt ttgttgataa
    22081 tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg
    22141 accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac
    22201 agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga
    22261 aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc
    22321 tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt cagaatggtg
    22381 ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc
    22441 atttgcactg gagaatttgt cagccaggtg tcaagcagat ccgccgtatc aagcatttta
    22501 tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg
    22561 actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg attttataca
    22621 gtatatttct tttcggttga gaaatcaaca tcagcaataa agacacacgc aaacgttttc
    22681 gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg
    22741 ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg
    22801 gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta tgccgacgca
    22861 ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta
    22921 cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc
    22981 accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca
    23041 acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg
    23101 cccacttcca cttatcgttt tgccgatagc gaaagccttt tccgcgaggc ggttgccgcc
    23161 attggctatc cctgcattgt aaaaccggtg atgagctctt ccggcaaggg gcagacgttt
    23221 attcgttctg cagagcaact tgctcaggca tgggaatacg ctcagcaagg cggtcgcgcc
    23281 ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccctgcta
    23341 accgtcagcg cggtggatgg cgtccatttc tgtgcaccag taggtcatcg ccaggaagat
    23401 ggcgactacc gtgaatcctg gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg
    23461 caggagattg cccgtaaagt ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag
    23521 ctgtttgtct gtggtgatga ggtgattttc agtgaggtct cccctcgtcc acatgatacc
    23581 gggatggtga cgttaatttc tcaagatctc tcagagtttg ccctgcatgt acgtgccttc
    23641 ctcggacttc ctgttggcgg gatccgtcag tatggtcctg cagcttctgc cgttattctg
    23701 ccacaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat
    23761 ttgcagattc gtttatttgg taagccggaa attgatggca gccgtcgtct gggtgttgcg
    23821 ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag
    23881 gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg
    23941 ggcgttttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc
    24001 ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca
    24061 gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca cccgtcgggc
    24121 agaaacggac ctgggagaac ggacccgcga tcgcctgcag ggctttcacg ccgccgttag
    24181 cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg
    24241 aaacagtgct gatccccgga atcagaggaa tagtcccttc ggtagcagct ttcagcagcg
    24301 gctcggtcag acccgggcta attgcgaact gtgcacccgc ttcagtgact tctgccagct
    24361 gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag
    24421 cacggatagc gtcaactgca cactcggtac gcagagtcac ttccagaacg cgcaccccac
    24481 cagcaaccaa cgcttttgcc atcggcactg cgtgttccag tttttttacc acgataaccg
    24541 gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag
    24601 ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt
    24661 cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg
    24721 acgcgctcag gtcaggaatg cgcggttcgc gagcagccag ttccgcttcg tctaccagca
    24781 gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca
    24841 gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact ttacctgaag
    24901 cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac
    24961 caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac
    25021 ggacaacaac gacacaatcg cggtccagca aacccgcttc aaaggccggc ataacgtcat
    25081 gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctcaacc ggcacggcag
    25141 aggttttcat aaccgcacgg cccaggttac cgcttaacac ttttgtccca ccatgatgag
    25201 agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag tgatttttcc gccccttccc
    25261 gccagtccag ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc
    25321 ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca cgcaccagaa
    25381 ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac
    25441 gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac
    25501 cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt agtggaacca ccggtcgcca
    25561 gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc
    25621 attcattacc attaccggtc atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat
    25681 cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc cccataaatt
    25741 ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatggt
    25801 aagacgcggc ttctgactcc agtaaggcca tgcggtccac tttaccttcg gcataaagct
    25861 ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa
    25921 acaccgcagg caaatgacca aacgacaggg ctgccatcgt cagacccggg acaatcttgt
    25981 cgcacacacc gaggaacaga gcaccatcaa acatgttatg ggacagcccg accgccgcag
    26041 acatcgctat cacttcgcgg cttagcagcg acaattccat tccatcctgc ccctgggtga
    26101 caccatcaca catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca
    26161 gggctttacg aatgatttct ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt
    26221 tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt
    26281 ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa
    26341 cggtcgaagt tttcgcttgt tctatccggg cgagataagc agagcgagtc tcgcgcgaac
    26401 gttcaatgat tcgatttgtt acgcgtaaca attgtggatt cataaaggct cctgaaattg
    26461 agttgtcaga gcaggatgat tcacaacgcg tttcattcag aggatttatg actgaaacgc
    26521 ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg
    26581 ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg
    26641 ttaccggtaa aataaccata aaggataagc gcagatatta ctcaaactca ttccaggaac
    26701 gaccatcacg ggtaatcatc gccaccgagg caacgggtcc ccaggttccg gcctgatacg
    26761 gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg
    26821 cttcttccac ttcgtcgcga cgaacaaaca gtgcctgaat accacgcatg gtttccagca
    26881 gcaaacgttc ataggcatcc gccagatgcg tctgattaaa ggtttctgaa tagctcagat
    26941 ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct
    27001 ggatatccac gccttcatca ggttgcagac ggatagtcag tttattctgc ggcagatcct
    27061 gccacgattc tttaaacaga ttcagttcag gtgttttgaa atagaccacg acttcagaac
    27121 atttggtcgg cagacgttta ccagtacgaa ggtagaatgg cacaccggcc cagcgccagt
    27181 tatcaatgtc gacgcggatc gctacgaagg tttctgtatt gctgctcttg ttcgcgccct
    27241 cttcttccag atatcccggc acttttttgc cctgggcgaa gcccgcagta tattgcccgc
    27301 gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg cagagacttc agtactttca
    27361 ctttttcatc gcggatgctg tctgcgctca ggtcagacgg cggagacatc gcaatcatgc
    27421 agagaatttg cagcaggtgg ttttggatca tatcgcgcat ctgaccggct ttatcaaaat
    27481 agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg
    27541 tgcgattgtc ccagttattc acaaacagag agttagcaaa acgcagcgcc aacaggttca
    27601 gcactgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt
    27661 cgccaacctg atcgttgatt tcccgcgagg tcgccagcga cgtccccagc ggtttctcca
    27721 tgactacgcg tgccggttta gcattcagtt ttgcctcacc aagccctttg caaattgcgc
    27781 caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca
    27841 tcgcgccgag acggctgaat gcagcagtgt cattgacatc gagattacaa aaatccagac
    27901 gtgcacttag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg
    27961 cctcgcggac aactttggta taagccgctt tatcccagtc agcacgccct acgccgataa
    28021 tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt
    28081 tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt
    28141 gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac
    28201 ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct
    28261 tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc
    28321 atcgttttcc attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt
    28381 ttttatctgc ttttgtcatt aacacggcac acaggcgtaa tatctgacaa aactgcattt
    28441 cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc
    28501 tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg
    28561 tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctatg gcactggaag
    28621 ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc
    28681 ctgattttaa acttcatctg gcacagagtc tggcgaatgg cactccctat gttaatcgca
    28741 atgtcaatga agatgacagc gttgaatcat acacagggaa aatatttgag tccgcaatgg
    28801 caacgcttga tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact
    28861 tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc gccgttgccc
    28921 acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg
    28981 tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc
    29041 acactggaag aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcca
    29101 tggtgattgc cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta
    29161 ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc
    29221 tgaccgtgat agatgtgctg gcgacaggat ttactttgcg acgcggtgca aaattcagag
    29281 ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta
    29341 atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat
    29401 gagattaatt tatcgccatc gcggcgttat ttcattcgga tttcatgttc aagcaacacc
    29461 tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt
    29521 accacgttag gcccagcaac agatcgcgat aataaccttg aaaaagttat cgcggcaggt
    29581 gccaacgttg tacgtatgaa cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg
    29641 gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc
    29701 caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg
    29761 gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt
    29821 atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg acatcctgct gctggacgat
    29881 ggtcgcgtcc agttaaaagt actggaagtt cagggcatga aagtgttcac cgaagtcacc
    29941 gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct
    30001 gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat
    30061 tacctggctg tctccttccc acgctgtggc gaagatctga actatgcccg tcggctggca
    30121 cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc
    30181 agccaggatg caatggatga catcatcctc gcctctgacg tggtaatggt tgcacgtggc
    30241 gacctcggtg tggaaattgg cgacccggaa ctggtcggca ttcagaaagc gttgatccgt
    30301 cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc agatgatgga gtcaatgatt
    30361 actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt
    30421 actgacgctg tgatgctgtc tgcagaaact gccgcagggc agtatccgtc agaaactgtt
    30481 gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa
    30541 caccgtctgg acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac
    30601 gcagctaacc acctgaaagg cgttacggcg attattacca tgaccgaatc gggtcgtacc
    30661 gcgctgatga cctcccgtat cagctctggt ctaccaattt tcgccatgtc gcgccatgaa
    30721 cgtacgctga acctgactgc tctctatcgt ggcgttacgc cggtgcactt tgatagcgct
    30781 aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tgcgcgataa aggttacttg
    30841 atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact
    30901 aataccacgc gtattttaac ggtagagtaa gtacgttgcc ggatgcggcg aaaacgccac
    30961 atccggccta cagttcaatg atagttcaac agatttcgaa tattctgaag caaacttgaa
    31021 cttatcatca ggcgaaggcc tctcctcgcg agaggctttt ttatttgatg ggataaagat
    31081 ctttgcgctt atacggctgg atttcgcccg gtttgcgagt tttcagcaat tttagtatcc
    31141 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
    31201 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
    31261 gacgatgcgt cttgccatca taaatcggaa acagcggtac aacgcgcgca cggcacactt
    31321 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
    31381 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
    31441 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
    31501 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
    31561 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
    31621 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
    31681 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
    31741 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
    31801 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
    31861 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
    31921 aagcgatacc cgccatcgct gctacgccca accatgctcc ccagtagcgc gggtggcgaa
    31981 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
    32041 agtttcggat aaggcaaaaa tcaatctggt gatagtgtag cggcgcaact tgccccgcac
    32101 caaataaaaa aagccggtac tgactgcgta ccggctgcga atggatgtta attaatcaaa
    32161 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
    32221 aagcccttca gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
    32281 tacttcatag tgcagatgcg gcccagttga acgtccggta ttaccggaaa gcgcgatacg
    32341 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
    32401 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
    32461 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatagcgaa
    32521 atcaacacct ctgtgtggtg caacgcgacc tgtcaccgga ttagtacgac gcgggttaaa
    32581 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
    32641 agtaccgtta cgatcgtaga atttgccatc ctcagcgcgg atagcgtaat aatctttacc
    32701 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
    32761 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
    32821 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
    32881 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
    32941 catttcgctg gtcattttaa aaccgttagc agcagtacgg tcataggttc gggtttcacg
    33001 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
    33061 ttgttgaccg attttcagat tacgcaattc tttgtcggca gcagccagtt gggtgatatc
    33121 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
    33181 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
    33241 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
    33301 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcacggt gataaacata
    33361 aggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
    33421 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
    33481 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
    33541 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
    33601 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
    33661 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
    33721 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc caaactgttt
    33781 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
    33841 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
    33901 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
    33961 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
    34021 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
    34081 cagcagcggt ttcacatctt ctagctgtgc aatcgttacc tgtttcgctt ctggtaattt
    34141 acttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
    34201 gttctgtaag cgttttacat ccgatggacg cagtgaataa tcatgttctg aagcgccgtc
    34261 aggcagtaaa acctccgttt ctgttacacc atcagcaatg gcagaagcga tgaacccaac
    34321 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
    34381 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
    34441 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
    34501 atgacaagtc tggtttccct ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
    34561 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
    34621 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
    34681 atcaagcgta atggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
    34741 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
    34801 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
    34861 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
    34921 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
    34981 gaccttattg accagttgcg tcgcgaactg gattgtggcg ttttaatggt ctctcacgat
    35041 ctgcatctgg taatggcaaa aaccgatgaa gtgctgtgtc tgaatcacca catttgttgt
    35101 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
    35161 ggtgctgaac aactgggtat ctatcgccat catcataatc atcgtcacga tttacaggga
    35221 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
    35281 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
    35341 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
    35401 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
    35461 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
    35521 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
    35581 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
    35641 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
    35701 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
    35761 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
    35821 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
    35881 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
    35941 ccttttccgc gttttacgat acgccggcgg gtccgtcggt ggtgctatgt gcggcactgt
    36001 tatttattct cagtatgatg aaaaagcagg ccagctaatc tatcgctgaa cacatttgtc
    36061 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gcctggtaag
    36121 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
    36181 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
    36241 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat caaataaggt tccagcacat
    36301 cctcaatggt ttcacgttct tcgccaatgg ctgccgcaag gttatccaga cctaccggcc
    36361 caccaaagaa cttatcgatt accgccagca ataacttgcg gtccatataa tcgaaacctt
    36421 cagcatcgac attcaacata tccaacgcct gagcagcgat atctgccgag atggtgccat
    36481 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atgcgcggcg
    36541 taccgcgagc tcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
    36601 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgct
    36661 gcacaatacc aaaacgatcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
    36721 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
    36781 tcatgatatc cagttggtag tcttccattg ccggatacaa cacctcttcc accactggcg
    36841 aaagacggtg gatctcatca ataaacaaca cgtcatgcgg ttcaaggtta gtgagcatcg
    36901 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
    36961 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccgaaaatca
    37021 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
    37081 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
    37141 ccacatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
    37201 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg
    37261 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
    37321 ggcaaccgct tcctgttcag catcgtcggt cgtcgggctg gcaggtgacg tgagtaccag
    37381 gtcggcggct ggcgtaaaga gatcgccatg caaaccttta aatcggtctt tcatttcaac
    37441 aatcaagcgt tcggcggttt ttttgccaat acccggcagt ttcaccagtg cccccacttc
    37501 ttcacgctca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
    37561 caacttcggg ccgacgccgt tggttttgat caactctttg aacaatgtgc gctcttgttt
    37621 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
    37681 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
    37741 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
    37801 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
    37861 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggcgt cccaacgatc
    37921 tgaaaaatgg cgca
//
LOCUS       DAZFFI010000037        37577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFFI010000037 DAZFFI010000000
VERSION     DAZFFI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.037"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..1655
                     /gene="aroP"
                     /locus_tag="ACHJ12_002179"
     CDS             282..1655
                     /gene="aroP"
                     /locus_tag="ACHJ12_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4989958.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1698..2552)
                     /gene="ampE"
                     /locus_tag="ACHJ12_002180"
     CDS             complement(1698..2552)
                     /gene="ampE"
                     /locus_tag="ACHJ12_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4989959.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2549..3100)
                     /gene="ampD"
                     /locus_tag="ACHJ12_002181"
     CDS             complement(2549..3100)
                     /gene="ampD"
                     /locus_tag="ACHJ12_002181"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4989960.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3188..4081
                     /gene="nadC"
                     /locus_tag="ACHJ12_002182"
     CDS             3188..4081
                     /gene="nadC"
                     /locus_tag="ACHJ12_002182"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4989961.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4284..4724
                     /gene="ppdD"
                     /locus_tag="ACHJ12_002183"
     CDS             4284..4724
                     /gene="ppdD"
                     /locus_tag="ACHJ12_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4989962.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4734..6119
                     /gene="gspE"
                     /locus_tag="ACHJ12_002184"
     CDS             4734..6119
                     /gene="gspE"
                     /locus_tag="ACHJ12_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4989963.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6109..7311
                     /gene="hofC"
                     /locus_tag="ACHJ12_002185"
     CDS             6109..7311
                     /gene="hofC"
                     /locus_tag="ACHJ12_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4989964.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7346..8389)
                     /gene="guaC"
                     /locus_tag="ACHJ12_002186"
     CDS             complement(7346..8389)
                     /gene="guaC"
                     /locus_tag="ACHJ12_002186"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4989965.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8545..8589)
                     /locus_tag="ACHJ12_002187"
     CDS             complement(8545..8589)
                     /locus_tag="ACHJ12_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989966.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8614..9234
                     /gene="coaE"
                     /locus_tag="ACHJ12_002188"
     CDS             8614..9234
                     /gene="coaE"
                     /locus_tag="ACHJ12_002188"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4989967.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9234..9977
                     /gene="zapD"
                     /locus_tag="ACHJ12_002189"
     CDS             9234..9977
                     /gene="zapD"
                     /locus_tag="ACHJ12_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4989968.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9987..10184
                     /gene="yacG"
                     /locus_tag="ACHJ12_002190"
     CDS             9987..10184
                     /gene="yacG"
                     /locus_tag="ACHJ12_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4989969.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10284..10673)
                     /gene="mutT"
                     /locus_tag="ACHJ12_002191"
     CDS             complement(10284..10673)
                     /gene="mutT"
                     /locus_tag="ACHJ12_002191"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4989970.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10733..13438)
                     /gene="secA"
                     /locus_tag="ACHJ12_002192"
     CDS             complement(10733..13438)
                     /gene="secA"
                     /locus_tag="ACHJ12_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4989971.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13500..14012)
                     /gene="secM"
                     /locus_tag="ACHJ12_002193"
     CDS             complement(13500..14012)
                     /gene="secM"
                     /locus_tag="ACHJ12_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4989972.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14243..15160)
                     /gene="lpxC"
                     /locus_tag="ACHJ12_002194"
     CDS             complement(14243..15160)
                     /gene="lpxC"
                     /locus_tag="ACHJ12_002194"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4989973.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15261..16412)
                     /gene="ftsZ"
                     /locus_tag="ACHJ12_002195"
     CDS             complement(15261..16412)
                     /gene="ftsZ"
                     /locus_tag="ACHJ12_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4989974.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16473..17735)
                     /gene="ftsA"
                     /locus_tag="ACHJ12_002196"
     CDS             complement(16473..17735)
                     /gene="ftsA"
                     /locus_tag="ACHJ12_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4989975.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17732..18562)
                     /gene="ftsQ"
                     /locus_tag="ACHJ12_002197"
     CDS             complement(17732..18562)
                     /gene="ftsQ"
                     /locus_tag="ACHJ12_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4989976.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18564..19484)
                     /gene="ddlB"
                     /locus_tag="ACHJ12_002198"
     CDS             complement(18564..19484)
                     /gene="ddlB"
                     /locus_tag="ACHJ12_002198"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4989977.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19477..20952)
                     /gene="murC"
                     /locus_tag="ACHJ12_002199"
     CDS             complement(19477..20952)
                     /gene="murC"
                     /locus_tag="ACHJ12_002199"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4989978.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFNEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21006..22073)
                     /gene="murG"
                     /locus_tag="ACHJ12_002200"
     CDS             complement(21006..22073)
                     /gene="murG"
                     /locus_tag="ACHJ12_002200"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4989979.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22070..23314)
                     /gene="ftsW"
                     /locus_tag="ACHJ12_002201"
     CDS             complement(22070..23314)
                     /gene="ftsW"
                     /locus_tag="ACHJ12_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4989980.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23314..24630)
                     /gene="murD"
                     /locus_tag="ACHJ12_002202"
     CDS             complement(23314..24630)
                     /gene="murD"
                     /locus_tag="ACHJ12_002202"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4989981.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24633..25715)
                     /gene="mraY"
                     /locus_tag="ACHJ12_002203"
     CDS             complement(24633..25715)
                     /gene="mraY"
                     /locus_tag="ACHJ12_002203"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4989982.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25709..27067)
                     /gene="murF"
                     /locus_tag="ACHJ12_002204"
     CDS             complement(25709..27067)
                     /gene="murF"
                     /locus_tag="ACHJ12_002204"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4989983.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27064..28551)
                     /gene="murE"
                     /locus_tag="ACHJ12_002205"
     CDS             complement(27064..28551)
                     /gene="murE"
                     /locus_tag="ACHJ12_002205"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4989984.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28538..30304)
                     /gene="ftsI"
                     /locus_tag="ACHJ12_002206"
     CDS             complement(28538..30304)
                     /gene="ftsI"
                     /locus_tag="ACHJ12_002206"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4989985.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30320..30685)
                     /gene="ftsL"
                     /locus_tag="ACHJ12_002207"
     CDS             complement(30320..30685)
                     /gene="ftsL"
                     /locus_tag="ACHJ12_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4989986.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30682..31623)
                     /gene="rsmH"
                     /locus_tag="ACHJ12_002208"
     CDS             complement(30682..31623)
                     /gene="rsmH"
                     /locus_tag="ACHJ12_002208"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4989987.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31625..32083)
                     /gene="mraZ"
                     /locus_tag="ACHJ12_002209"
     CDS             complement(31625..32083)
                     /gene="mraZ"
                     /locus_tag="ACHJ12_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4989988.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32685..33689)
                     /gene="cra"
                     /locus_tag="ACHJ12_002210"
     CDS             complement(32685..33689)
                     /gene="cra"
                     /locus_tag="ACHJ12_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4989989.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVCE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33771..33857)
                     /locus_tag="ACHJ12_002211"
     CDS             complement(33771..33857)
                     /locus_tag="ACHJ12_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4989990.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33869..34360)
                     /gene="ilvN"
                     /locus_tag="ACHJ12_002212"
     CDS             complement(33869..34360)
                     /gene="ilvN"
                     /locus_tag="ACHJ12_002212"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4989991.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34363..36087)
                     /gene="ilvI"
                     /locus_tag="ACHJ12_002213"
     CDS             complement(34363..36087)
                     /gene="ilvI"
                     /locus_tag="ACHJ12_002213"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4989992.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36206..36250
                     /locus_tag="ACHJ12_002214"
     CDS             36206..36250
                     /locus_tag="ACHJ12_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4989993.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36405..37349)
                     /gene="leuO"
                     /locus_tag="ACHJ12_002215"
     CDS             complement(36405..37349)
                     /gene="leuO"
                     /locus_tag="ACHJ12_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4989994.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9055 a        10468 c         9173 g         8881 t
ORIGIN      
        1 ttgtgttttt taattcaatt aaaacgaatt taaattcatt ctacatattg agaggggttg
       61 aggctgagct ttacaaacgg tttcttttta agcaactcat cttcaaccat gcataaagcg
      121 ggtgcattcg ctgccgcata ccattattct tgatctgacg gaagtctttt tgtaacaatt
      181 caaacttctt tgatgtaaac aaattaatac aacaaacgga attgcaaact tacacacgca
      241 tcactgcgta gatcaaaaaa acaaccaccg cacgaggttt catgatggaa ggtcaacagc
      301 acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg
      361 gcgcgatagg gaccgggtta ttcctgggta gcgcctccgt aatacagtcc gcagggccag
      421 ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctgg
      481 gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact
      541 ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg
      601 ccatggctga gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccca
      661 cctgggtttc tgccgccgta ttctttgtgg tgattaacgc catcaacctg accaacgtta
      721 aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttatcgcg gtggtagcga
      781 tgatcatctt cggcggctgg ctgctattca gtggcaacgg cggcccgcag gcgaccgtta
      841 gcaacctgtg ggatcagggt ggtttcctgc cgcacggctt caccgggctg gtgatgatga
      901 tggcgattat catgttctcg ttcggtggtc tggaactggt ggggatcacc gcagcagaag
      961 ctgataaccc ggagcaaagt ataccgaaag caactaacca ggttatctac cgcatcctga
     1021 ttttctatat tggttcgtta gccgttctgc tctcactgat gccgtggacc cgcgttaccg
     1081 ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt gtggcgaatg
     1141 cgctgaacat cgtggtactg actgcggcgc tctccgtgta caacagctgc gtatattgca
     1201 acagccgtat gctgtttggt ctggcacaac agggtaatgc gccaaaagcg ctggcgtctg
     1261 tcgataaacg tggtgtacca gtaaatacca ttctggtgtc tgcactggta acggcgttgt
     1321 gcgtactgat taactacctt gccccagagt ccgctttcgg actgttaatg gcgctggtgg
     1381 tatctgcact ggtaatcaac tgggcgatga ttagcctggc gcatatgaaa ttccgtcgcg
     1441 ccaagcagga acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact
     1501 ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg
     1561 cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta
     1621 aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccgtacag
     1681 ggtgagggca ataatcttta caccaacgcc ccgtaaatcg tcagtagtgc aatcaccacc
     1741 acgaccacga acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt ctccacctta
     1801 tcgacatgcg gttcacgcgc cagagagaac tgcgccagac gcgttaacac ctgatactgc
     1861 gaagtatgga aatcacccag cgaagcaaac caggccggta acgctttctc accatgaccg
     1921 atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg
     1981 gcatcaatgc cggactgtaa acgatgatgc ggcgtctgat atcgcgccag ccagtattgc
     2041 catgcacgca aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc
     2101 agccagaaca gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc
     2161 tcacgcaaat actcacgttc gtcgcagcct gccgggacgc cgtgaatcat ggtgagttcg
     2221 ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga
     2281 taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagt
     2341 agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc
     2401 atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac ccgccgaaag
     2461 aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa
     2521 attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agcacacgaa
     2581 accgtgccca atcaaatgca ggaccgggat cggttttccg atccggcgca atatcacaat
     2641 ggcccgtcat gtttttagcg atatccggat agcaatcaat cagtgcccgc gtaaccgccg
     2701 caagctgttg atactgcgca tcggtatacg ccagcgtatc ggtgccttca agctcaatcc
     2761 caatagaaaa atcattgcag cgttcgcgcc cctgatactg agagactccc gcatgccatg
     2821 cacgtttatc gaaaggaaca tactggacta tttcaccatc acggcgaatc aaacagtgag
     2881 cggagacgcg caaatgggcg atctcagcaa agaaaggatg tgcctgcgga tcaatagttc
     2941 cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt
     3001 gcaccaccag cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa
     3061 cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg
     3121 gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag
     3181 ttttaacatg ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg
     3241 cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac
     3301 agtcgatgcc aacaatgata ttacggcaaa acttttaccg gaaaattctc gctctcatgc
     3361 cacggtgatc acccgcgaga atggcgtctt ttgcggcaaa cgctgggttg aagaggtgtt
     3421 tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat
     3481 caatgccaat caatccttgt tcgaacttga aggcccatcc cgcgtgctgt taacgggcga
     3541 acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg tacgccacta
     3601 tgtcgaattg ctggaaggca ccaacacgca gttgttggat acgcgcaaaa ccttacccgg
     3661 cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg
     3721 gctttctgat gccttcctga tcaaagaaaa ccatattatt gcctccggct cagtgcgcca
     3781 ggcggtcgaa aaagcgtcct ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa
     3841 tctggaagaa cttgatgaag ccctgaaagc aggagccgat atcatcatgc tggataactt
     3901 cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt
     3961 gtctggcaac gtcactgaca aaacactgcg tgaatttgcc gaaacgggcg tggactttat
     4021 ctccgtcggt gcgctaacta aacacgtaca agcactcgac ctttcaatgc gttttcgcta
     4081 atagttgaca gatttatccg ctccatcgcg ggcggataat ctccccttcc ccaactttct
     4141 tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga aataaaaaac ctcggaatct
     4201 gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg gcgtttgcca aagtagcgcc
     4261 aaccaaatca aggagcgaaa cagatggaca agcaacgcgg ttttacactt atcgaactga
     4321 tggtggttat tggcatcatt gccattttaa gcgccattgg tattcccgct tatcaaaact
     4381 acctgcgcaa agccgcactc accgacatgc tacaaacctt tgtgccttac cgtaccgccg
     4441 tagagttgtg cgcgctggaa catggtggat tagatacctg cgacggtggc agcaatggca
     4501 ttccctcgcc taccaccacc cgctatgttt cagccatgag tgtggcaaag ggcgtggtgt
     4561 cgctgaccgg gcaagaaagt ctcaatgggc taagcgtcgt catgacaccg ggttgggata
     4621 acgcaaacgg cgtcaccggc tggacgcgca actgcaatat tcaaagtgac agcgcattgc
     4681 agcaagcctg cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg gcaatgaata
     4741 ttccacagct cactgccctg tgtctgcgtt atcatggagt cttgctggat gccagcgaag
     4801 aggtggttca tgttgcggta gtcgatgcac cttcgcatga gctactggac gcattgcatt
     4861 tcgctaccac caaacgtatt gagatcacct gctggacgcg ccaacaaatg gaaggtcacg
     4921 ccagtcgcac acaacagaca ttgcccgtag ctgttcagga gaagcatcag cccaaagcag
     4981 agttgctgac tcgaacgtta caatctgcgc tggaacaacg cgcgtctgat attcatatcg
     5041 aaccagcgga caatgcctac cgcatccgct tgcgtatcga cggcgtattg catcctttac
     5101 cggatgtttc accggatgcc ggagtcgcat taaccgccag attaaaagtg ctgggaaacc
     5161 tggatattgc ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa
     5221 acgccgtctc atttcgtatt gcgaccttac catgtcgagg tggtgaaaag gtggtattaa
     5281 ggttgttaca gcaggtgagt caggcactgg atgtcaacac gcttggaatg cagccgttac
     5341 aactggcgga ctttgctcat gccttgcaac aaccacaggg actggtgctg gtaactggcc
     5401 ctaccggcag cggcaaaacg gtcacgcttt atagtgccct gcaaaagctg aataccgctg
     5461 acattaatat ttgtagcgtc gaagatccgg ttgagatccc catagccgga ctaaaccaga
     5521 cgcaaatcca tccgcgtgcc gggctcacct ttcagggcgt tttgcgtgcg ttattgcgcc
     5581 aggatcctga cgtcatcatg atcggagaga tccgcgatgg cgaaacagca gagatcgcta
     5641 ttaaagcggc gcaaactggt cacctggtgt tgtctaccct acacactaat tccacctgcg
     5701 aaacgctggt acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta
     5761 cgctggtaat agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag
     5821 gggagcccat ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac
     5881 ccggttgtgt acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc
     5941 ccataacgcc ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa
     6001 cgcacgcacg acaggcgggt atgtgtacgc tttttgaaaa cggctgcctg gccgtggagc
     6061 aaggcttaac cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag
     6121 caactctggc gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatgg
     6181 gcagagagcc gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc
     6241 ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt
     6301 catcaactgg cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg
     6361 gcggaacagc atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc
     6421 gaacagggca ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc
     6481 tatcaggcga tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa
     6541 ctggcgcgtc agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt
     6601 tatcccatca tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt
     6661 ctgccggagt ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag
     6721 gggatcatga cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc
     6781 tttctgctgg cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg
     6841 cagaaattgc tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg
     6901 cagattttta cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta
     6961 gagagcgtca gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag
     7021 cacgatatca gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc
     7081 ccgctctgtt tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta
     7141 gacaacctcg cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc
     7201 ttactggaac cggcgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg
     7261 atgtatctgc caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt
     7321 gttgctccac accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct
     7381 gcacacggat aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg
     7441 tacaagctga acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca
     7501 gcggcagctt aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac
     7561 gtttcatcgc agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct
     7621 caacgatgcg accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat
     7681 cggcaccgcc gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga
     7741 cgatcattcc gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg
     7801 gataaccgac gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt
     7861 taacgatatc agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc
     7921 cagcacaaat ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt
     7981 gttcggaata accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga
     8041 gaatctgttt agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt
     8101 tcagcacatc agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt
     8161 tatgcacagc agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta
     8221 cggtgtccat atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga
     8281 aggtgaattg acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc
     8341 ggatgagaac gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc
     8401 tggggttaat ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat
     8461 caatgcggcg caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct
     8521 ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt
     8581 gataatcatg atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga
     8641 ggcattggca gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc
     8701 attgatgccg atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc
     8761 attgctgatc actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc
     8821 ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat
     8881 ccgctgattc agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg
     8941 tgggttgtgc cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg
     9001 gtggatgtca gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc
     9061 gagcatgtcg aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca
     9121 gatgacgtca ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg
     9181 cacgcacact atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga
     9241 cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt
     9301 ttttgattca gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt
     9361 tcttccgtaa tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc
     9421 tgttgaaaga acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg
     9481 tggaccagag ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa
     9541 tttccgcgcc gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc
     9601 agcgactgag catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc
     9661 tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc
     9721 cgctcaccca ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta
     9781 aacaaaccag cctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc
     9841 tgaatctgtc gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg
     9901 ccattcgttt tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg
     9961 aactggcctg ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct
    10021 gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt
    10081 gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg
    10141 atctttccga aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg
    10201 cacgtaggcc tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat
    10261 gcggcgtgaa cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg
    10321 gctggcggaa aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct
    10381 tctttacccc acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc
    10441 ctgtccggga attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg
    10501 acttcttcct gaagttcacg caccaccgcc tgttccggcg tttcgcccat ttcaatttta
    10561 ccgccgggaa actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag
    10621 atttcattgt tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta
    10681 aacctataaa aaaagcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca
    10741 ggcgaccatg gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc
    10801 ctactttgcg ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct
    10861 gatggctaag ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca
    10921 gctcctcaac ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat
    10981 attttaacga ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct
    11041 tcggatcttt ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg
    11101 ccaggtgctc tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac
    11161 gcatcatctc agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca
    11221 gaattcgctc acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa
    11281 ttggcaaatc gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca
    11341 tttcttccag tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac
    11401 gaatgctgtt gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt
    11461 aaatggcgcg acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt
    11521 cgaagttacg gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt
    11581 gttcaatggc ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg
    11641 aagcaaaaat acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat
    11701 ccccctgacg accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct
    11761 cggtaccgat gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt
    11821 cggctttaat tttttcaatt tgctctacgg tcggattttc cagcgcggca acttctgcct
    11881 gccagctacc accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca
    11941 ccgcagccgg ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt
    12001 tcaggacgtt gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt
    12061 cgatggagat agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt
    12121 caatgatcgc ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac
    12181 gaatcattgg acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt
    12241 cgaaagcttc ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt
    12301 agttctggaa ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt
    12361 tcgcttccac agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg
    12421 tgtgttcgtc aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa
    12481 acagcgcatg agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag
    12541 agtacagaga ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca
    12601 ccagaccacg ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt
    12661 cgccctggaa ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat
    12721 tcacgcgttt atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac
    12781 gcgcttcatc gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct
    12841 gtacacgttc ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt
    12901 tgttcgtacc gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc
    12961 ccggcaggtt gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg
    13021 cgtcacgttg cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg
    13081 cgttcaggta agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg
    13141 cgatgcagcg ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac
    13201 caaagacgcg cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca
    13261 gcacttcgcc tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt
    13321 cggagagttt ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc
    13381 ggcgcagggt gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa
    13441 taaaatctca aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt
    13501 taggtgaggc gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg
    13561 ctgaattttg cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc
    13621 gtgccttcct gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga
    13681 agaggcaaag attcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga
    13741 cggattaccg tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg
    13801 cggcgtgtgt tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg
    13861 tggttgcggg ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc
    13921 aaacctaaac tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca
    13981 aactgtcgcc agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac
    14041 gtatttagcg cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag
    14101 ttgtgcggaa aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa
    14161 ttcaaaaaaa aacgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt
    14221 ttatacgaca gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg
    14281 gcagttctgc gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag
    14341 cctgcagcag tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa
    14401 tattgtgacc acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt
    14461 cgtcttcaaa acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac
    14521 aatcgaagct gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga
    14581 aaccgaacgt acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg
    14641 catagcgctg gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg
    14701 aaaaaccatt gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct
    14761 ctttgatgcg aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca
    14821 ggtatacaaa cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga
    14881 taacaatgtt atcgatgccc aagcccgcga gagcagcatt gaggtgctct acggttgaaa
    14941 tccgtacatc atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg
    15001 catcggccgg gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt
    15061 tggccggcgc agggcgtaac gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg
    15121 tcgcctgaac gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta
    15181 cctatccaac cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa
    15241 acccaaattc cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga
    15301 taatccggct ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc
    15361 tgctcctggg tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga
    15421 acctgcttat tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca
    15481 acaacggtta cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca
    15541 gtcgcgttgt cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga
    15601 cgcaggtcga agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata
    15661 tcttccagca gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg
    15721 ctcgccacgc cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct
    15781 gcaaagtcca cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg
    15841 cctttcagta catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga
    15901 actttcagca gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca
    15961 gtgatcccct gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg
    16021 acgacagcaa cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca
    16081 cctgtaccgg taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc
    16141 gccgcacgca atgcatcgcg atcctcatca gccgcattgc ggccaacttc tggattagcg
    16201 ccagcgccca gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt
    16261 ttacgcagcg cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatgcgctcg
    16321 cgcaccatgt gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc
    16381 gcgtcattgg taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc
    16441 tgaggccgta atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg
    16501 agtcgcttga tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg
    16561 ttaagatgtg actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc
    16621 tgagcataat ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga
    16681 aacacgcgct gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca
    16741 atgcctgccg ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat
    16801 atctcttcgt tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg
    16861 gtctgacgtt gcagactccg cggcggacga ccacctacgc tcggcacttc cacgctctca
    16921 tcttttccaa cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg
    16981 cttggcggcg tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga
    17041 attaccttag tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca
    17101 ccgatatcga cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt
    17161 gatgccagtc cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg
    17221 acgatgtttt tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc
    17281 acgcccgaaa gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc
    17341 gggatcacat gcagcacacg atgctcatcg cgcacacgca ccgatttcgc ggtatggacg
    17401 acgttttcca catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca
    17461 ttctggcagc tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct
    17521 gccatcaatt ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg
    17581 ttcaccccgc ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc
    17641 ataccgtcgg gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt
    17701 cctactacca gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt
    17761 gttgctgagt agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat
    17821 aacgcaaatc aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg
    17881 gataaagttc tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct
    17941 taatatcgtt attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca
    18001 gagtaaatct gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat
    18061 tggcgctgcc ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg
    18121 gcacgctgaa ggtgtttcct tccgcgtcta ccatgtgttg atcattccac cgcgcaatcg
    18181 gcacatattc aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct
    18241 gcttaatcca cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca
    18301 taaaggtacc cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt
    18361 aatggcgttc accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc
    18421 agcccaacac gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga
    18481 tccccgccag acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg
    18541 tgttcagagc agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc
    18601 gagaagctca tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc
    18661 ggtgaggtat tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata
    18721 cgtccccatc ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg
    18781 gcctcttgtg acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac
    18841 ttcgcctcat aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct
    18901 tcaccgagta tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct
    18961 tcatcgtgct gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt
    19021 gacattccca cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca
    19081 gaaatttctg ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct
    19141 acccacggcg cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc
    19201 attgaaagcg cagatgccat cactccgctg ccggtataag gcaagcccat cagctcgagc
    19261 atgccctgca gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctgg
    19321 aagcccatcg acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcgtca
    19381 ataccgcctt cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc
    19441 tcagcggagg tcccgcccaa caggaccgcg attttatcag tcatgttgtt cttcctccgg
    19501 agtttgcggc ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc
    19561 cccctgaacg agaatcaggt cgttaccggt taataccggt gccagcatct cggctacctg
    19621 cgccgggtcc ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag
    19681 cgaacggctg tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa
    19741 cagggtatca acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt
    19801 aaaacggtgc ggctgaaaca gcattaccag gtttttatcc ggccagcctg cacgcgctgc
    19861 tttaatagtg gcgtccactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt
    19921 accgctttta ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgccc
    19981 agtcccctgg aagctctcca gcgcacgtaa aatagcctcg tcatcaattc cctcttccgt
    20041 agcaaccgca accgcagctg cggcgttcag cgcgttatga cgacctggcg cattcagggt
    20101 gacgcgcatc ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg
    20161 ctgataatct tctacacgca cgtcggcatc ttcgttgaag ccgtaagtcg tggtctgacg
    20221 tcccactcgc ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc
    20281 gtaaaacggc aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc
    20341 ctggtaggta tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag
    20401 atgcaggaac gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa
    20461 acgcgcatga acccccgccg cttttaccag cccaccgtta acgaaggttg ggtcgagtcc
    20521 cgcttctgcg tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc
    20581 aatggcgatg ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac
    20641 cggaatacgc gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tcgcgctgga
    20701 aacaacgacc acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc
    20761 cccaagattc attaactgtt gcgtgaccgg atttggcgct aaatcggaac cactgatctg
    20821 ataaccttca ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac
    20881 aaaatgtatg tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg
    20941 ttgtgtattc attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc
    21001 tacaattacg cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga
    21061 atggatgcag cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc
    21121 agggtgttgg cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc
    21181 gctttttcca gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca
    21241 aacaacgccg gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa
    21301 acgacgacat ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta
    21361 tgctgcggtt gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg
    21421 ctctgatgcc agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg
    21481 ttaagaatgc gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt
    21541 ccagccaaac gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc
    21601 accacttccg cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc
    21661 gccagccatt tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg
    21721 cctaacgacc acgcggccag accacctgga cctgatacat agccgcccat accgagtacc
    21781 acgtcaggtt tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc
    21841 agcggcgcag ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa
    21901 tcaatttcga tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc
    21961 cagcgaactt gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca
    22021 tgtccaccgg ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac
    22081 ctcgtacaaa cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca
    22141 tgatggctgt cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca
    22201 gacctttggt cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa
    22261 accagatgcc aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt
    22321 tgcggccaat cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca
    22381 caccgacata ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg
    22441 gcagatactc cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac
    22501 caaacgccat cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt
    22561 tccagaatgc ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg
    22621 ctgaaatgcc cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca
    22681 acatcgccag cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca
    22741 gtaacactgc caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac
    22801 gtacttcgtc gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg
    22861 tcagctccgc aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa
    22921 ccgagctacc cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg
    22981 cactgtagcg ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg
    23041 ccaggatcag atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt
    23101 gccctatggg cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg
    23161 tcagccacag taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc
    23221 ccatcaccca gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc
    23281 gcggcatttt caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc
    23341 aaactcattg cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga
    23401 gagcagaacc atatcgcccg gctgaacacg cggagccagc aagcgcatcg cctgttccat
    23461 agtttcggtt tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc
    23521 aaaacagtac aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc
    23581 cgatttacca tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa
    23641 cgccgcttcc gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt
    23701 gtgctccagc accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag
    23761 gctgctggcg cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt
    23821 gtagttatgc tgcccggaaa gtttcatctc tttcacgttc agcactttct cgcctttcac
    23881 tcgcagccag gtttctccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa
    23941 gctgacgcag cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac
    24001 gacacaaact ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc
    24061 aaacggatag cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg
    24121 taagctggaa gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc
    24181 agcatcaagc agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc
    24241 cgccgctttc gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt
    24301 aatcgccaca atcggtgctt gtgcttcgcg gcagaacagc tcgatatcgc caacgatttc
    24361 gattccggca tcagcggcag cgcttaagga tggatgcgcc agtgcaatac cgggactggc
    24421 gacaatcaga tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac
    24481 ggcttcgggt aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt
    24541 cacaccgcga gcgaggaaaa agtccacgca ggaaagccca gtgaggccca ggccgataat
    24601 gacgacattt ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca
    24661 atcagaacca gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag
    24721 cctttcagtt catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt
    24781 ttaaaggagc cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata
    24841 atcaccagca ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac
    24901 gccagcgaac ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag
    24961 cccagtcctg ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga
    25021 tacggtatat gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca
    25081 aaaccaccgg cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg
    25141 tttaccgcgt tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc
    25201 agctgcggca tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg
    25261 ccggcaaggt acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc
    25321 caacgagcga tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag
    25381 ccaataacac cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg
    25441 taagcccaca gcagtacgga gatcactatc gccgtcagga tcataatccc gcccatggtc
    25501 ggcgtaccgc gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag
    25561 gaaagttttt gcaaatgagc aatcatacgc gggcccatcc acaatgagat gaacagcgcg
    25621 gtcagcaggc tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa
    25681 tatttgacca aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta
    25741 ctacctcttc catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct
    25801 cagcaatcag tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa
    25861 cgccgctggc ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac
    25921 cagcagcttt tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt
    25981 ccgccatatc gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag
    26041 cggcggtcat tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg
    26101 ccagttggat ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga
    26161 tagcatcaag cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa
    26221 tattgtgacg ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg
    26281 taaattccgt accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg
    26341 cattgggtga gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt
    26401 cgttgttgtc ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac
    26461 ctttcgcttt cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt
    26521 tgttgaccag cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat
    26581 ggttcgcgcc aagttcaata actgcgtaat cgtattccgg cgttaagcgc aacagcgtca
    26641 tcggtacacc gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc
    26701 ttaaaatcgc cgccgtcatc tctttaacgg aggttttgcc ggaggacccc gtcagagcaa
    26761 ccacgcgcgc cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg
    26821 tatccttgac gattaactgc ggcaggtcga tgtccagcgg acggctaacc agtagtgcgc
    26881 ctgcgccgcc agctttcgcc tggtcggcaa aatcgtgggc atcaaaacgt tcgcctttca
    26941 gggcaacaaa caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg
    27001 tgatatctgc accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc
    27061 taatcatgca atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg
    27121 ctgattgcca acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt
    27181 ctctttagcc tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc
    27241 atgtccggca tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt
    27301 acgcgggtta tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat
    27361 cagtggacgc ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc
    27421 cgcacagtgc aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta
    27481 atccaccacc accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg
    27541 ttgcagacgc gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt
    27601 cgccagcgcg agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc
    27661 gccatcgccc caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc
    27721 gatcgctttt aaccagcgtc cgtgacagtt cggattgata tgatcttcca ttgataccgc
    27781 aaccgcgtcc ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat
    27841 cgcctgaccg caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat
    27901 atcaccatga taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt
    27961 caatgccgcc acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt
    28021 cgcgccctgc tccaccagcc ccgccagctc atgctgaaca tcgactgccg aaccggtagt
    28081 attttctgtc gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct
    28141 ggtttcgcca agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt
    28201 ggtgcccgtt acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc
    28261 cagtgcagat aaacgctcgt tgagctggct gagatagatg accggtacgc cgtgcatttc
    28321 acggatttca ccatcggtcg cctcatcttt cgcctctgca ataatggcag ccacaccttg
    28381 cgctatcgcc tgcgggatat atcgacgccc gtccgcctga tgacctacta cagctacaaa
    28441 gagatcgccc gccgcagcca cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg
    28501 tgcgtctggc acccacggag caagaaggtc gcgcaaatta cgatctgcca cctgtcccct
    28561 cgccttgatt aatcacaaat tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt
    28621 tcatggtacg caatacgccg cccatgatgg caccaaagac cggcgcggaa acggcgccgc
    28681 cgtagtattt acccgcctgc ggatcgttga taacaacaac cagcgcgaag cgcggctgac
    28741 tcgcaggcgc aacgcctgcg gtataagcaa tatatttatt gatgtagcga ccgtccggcc
    28801 cgaccttttt cgcggtaccg gttttaatgg cgatacgata gcctttaatc gccgctttca
    28861 cgccgccgcc gcctggtagc gccacgcttt ccatcatatg caccacggtg cgaacaatgg
    28921 attccgggaa gacacgttca ccgggaaccg gggggtcaac tttggtaatc gacagtgggc
    28981 gataaatgcc gtagctgccg atagttgcgt agactcgcgc taactgtaac ggtgttacca
    29041 ttagcccgta gccgaaagag aaggtggccc tctctatgtc agaccaccgt tgtttttgag
    29101 gatataagcc actgcgttct ccgaccaacc ccaaattggt cgcttttccc agtccaaaac
    29161 gtgagtaagt atctactaac gctgaggacg gcatcgctaa cgccagcttg gaaacaccga
    29221 cgttactcga cttctgtaat accccggtca gggttaattc gctgtagcgt gccacgtctt
    29281 tgatttcgtg gccgttaatt cgataaggaa tggtattgag taccgagttt tcccgcacca
    29341 cgccacgttg caacgcggtc attaccacca tcggtttaac cgttgagccc ggttcaaaca
    29401 cgtcggtgat ggtacggtta cgcatcgcct ctttcggcgt gccgctcaga ttgttagggt
    29461 tgtatgacgg gctgttagcc atcgccagca cttcaccggt gttgacatcc accagcacgg
    29521 cgctaccgga ttcagccttg ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg
    29581 cctgcaagcg ttcatcaata ctcaacgcca ggttgtgcgc tgcctggctg tcagtagaag
    29641 aaatatcttc aattacgcga ccatagcggt ctttacgcac aatgcgctca cccggctgcc
    29701 cggtaagcca tttatcgaaa ctcttctcaa cgccctcaat cccctgacta tcgacgttag
    29761 taaagccgat gaggtgagca gtcacttcgc cggatggata ataacggcga gattcttcac
    29821 gcagatgaat ccccggcagt ttcagttttt tgatgtagtc cgccatgtca gggtttacct
    29881 gacgcgccag ataaataaag cgccctttcg ggttggcgtt aatgcgggca gaaagctgat
    29941 ccagcggaat attgagcgcg ttagccagcg ccttccagcg gtcaccgacg ctgataccgc
    30001 cagcgtcatg cacttctttc gggtcagccc aaatcgcttt taccggcacg ctcaccgcta
    30061 acgggcgacc agaacggtca gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa
    30121 gagaacgcat gtcgccctct ttcaccagca tatccgggga gataacttgt aaccacgcta
    30181 cgcgtccgag cagaaaagcc agcgccagga gaatacagcc gcataacaac gcaaaacgcc
    30241 aactgataaa gttggcatgt tcttcctgac gttttggttt ctgcgttttc gccgctgctt
    30301 tcatgcgtcg cgtttatcct tatttttgca ctacgatatt ttcttgtgac ggatcaacat
    30361 gctgcatttg cagtttttcc gtggcgatcc tttccacccg gctatggtcg ccgagcgcat
    30421 tctcttcaag gatcaggttg cgccattcaa tgtctaaagc atctcgctcc agcaccagtt
    30481 gttcgcgctg agcggtcagt aaacgggtat ggtgcgccgt ggttaccacg gtcaccgccg
    30541 tcaaaataat gcaaatgaac aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac
    30601 cgataacacc aggcaatgca tggcgctcgt ggcttcccat cgatccttta actttgctta
    30661 gagcttctgt cactctgctg atcatgcgtt cgtcctctct gcgatacgca gaactgaact
    30721 acgggcacga ggattctcag ccacctcttc ttcgcccggc attaacttgc ctaatgctcg
    30781 cagctgacgg ccacccagtt ttttgagctg ctcttcagtc atcggtaacc ctgccggaac
    30841 ttgcggaccg cggctgtttt cacgcataaa acgtttcaca atacggtctt ccagcgagtg
    30901 gaagctgatg atcgaaagcc gcccacccgg ggccagcacg ttgagcgagc tttttagcgc
    30961 ctgctctatc tcctccagtt cactgtttac ccaaatgcgc accgcctgga aggtacgggt
    31021 cgcgggatgt ttaaacttat ctttcaccgg cgttgcagca gccacgactt ccgccagttc
    31081 tttggtgcgg gtcatcggct gttcgcggtt acgctcgaca atggcgcggg caatgcgttt
    31141 ggcaaaacgc tcttcaccat aggttttcaa tacccaagcg atatcggctt cttctgcggt
    31201 ttgtagccat tcagcggctg actgcccacg ggttgggtcc atacgcatgt ccagcggacc
    31261 atcgcgcata aaggaaaagc cgcgctcagc atcatcaagt tgcggtgaag agacgccaag
    31321 atcgaggaga atgccgtcga tcttgccgat aagatcgcgc tcggcaacgt attcgcccag
    31381 cgcggagaaa ggtccgtgga tgatggagaa gcgcggatca tcaatagtct tcgcaacggc
    31441 gatagcctgc gggtcgcgat cgatcgccag caaacgcccc tcttcgccaa gctgcgagag
    31501 gatcagacgt gagtgaccac cgcgaccaaa agtcccatca atgtagatgc catcaggacg
    31561 gatattgagg ccattaacgg cttcatccag cagcaccgta gtatgtttat agttttccat
    31621 cattttatag agacaagtcc tgcagtcgct ccgataagtc tccggtagcc aactgctctg
    31681 cgtcgatatc ttccttgacc tgttgatgcc aggttgtttc atcccacagc tcaaacttgt
    31741 tgaactgtcc aaccagcatc acttcttttg tcagcccggc atgttgccgc agtactggcg
    31801 cgattaacaa tcgacctgcg ccatccatct gacattcgct ggcatgacct aacagtaggc
    31861 gctgcacacg gcgctcaacc gggttcatgc tcgacagacg cgataatttt tgctcgataa
    31921 tttcccattc aggcaggggg taaagcagca ggcacgggtg atgaatgtca atggtgcaaa
    31981 ccatttgacc ggcagcgttc tcaagcagct gttcccggta acgggtaggc actgataagc
    32041 gccctttgct gtcgagattg actaacgttg ctccccggaa catgccagcc tcacccctta
    32101 ttcccacttt gccccacaat ttcccactga aaggagttta cggagcggag gaaaagcttg
    32161 tcaagccagt cacaaggcat accgaggtaa aaaacccgat gtttacggct aatatcagcg
    32221 ttgtttattt tacgtccaac gtgcgcagca aattaacgtt atgaatagct gtaagaaaaa
    32281 atccctcatc gtcatcaccg ttttaaaatg actcactatt ttccacagca aatataaagt
    32341 gtcagtttgc gacgcgagcg gcattttagg acatatcttc cccggttaac agtccctgtt
    32401 gcgtctgtgt ggcgcttgat gccagcgggt ttgcgcaaaa gcaaagaaaa gtgcttgtta
    32461 aatcggcaat tcatctctgc atgtaacaaa ataatgcaaa aacgcgctgt cgatatcact
    32521 taattattgt gaatttcagc tggtgagtta attaacgggc gggtttaaaa gaacttatca
    32581 ataatagtca ggaatagtct tatttacttt aaggatattg atgtccattc ccgtactcta
    32641 cgcgccagag ggaaattcac ctggcgcgta tttttgttcg cggcttagct acggctgagc
    32701 acaccgcgac gatagagatt acgtttaatg cgcgttaaac caggttttgg cttacgcggt
    32761 tcgtccaggc ttgccaggac aatctccagc acacgctctg cgacatcgcg gtgacgttga
    32821 gccactgcca gcaccgggca ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca
    32881 attgccaggt cagaaggcag tttaccatcg cgacgtagcg ttacatccat caccccttgc
    32941 aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg gatgcgtttc cagccatttt
    33001 tcgaataact gggcagccgc ctcccgctca tagctgttgg catacaggaa atgcacttcg
    33061 cgcggatcat ctttccaggc agtacggaaa ccttgttcac gcaggaagct gacagaaagc
    33121 tccggcagcg cgccaagata aagcaccgtc tcggcgggaa acttacgtaa ctcttccgcc
    33181 agcatttcgg catcatcctg atcggcacca accacgctgg tgaagtgttc acgatcgagg
    33241 gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc agcgttgata aaaaggatgc
    33301 tcaggaggca acgacgtcga aacaataatg gcatcgacct gacgctgtaa aaggtgctca
    33361 atacagcgca tttcgttgtc tggctgatct tctgagcagg caattagcag ttgataaccc
    33421 cgttgccgcg cctggcgttc aagatagtta gcgatgcggg tatagctggt gttctccaga
    33481 tcggggatca caagaccaat agaacgtgtg cgtccagcac gaagcccagc tgccacggcg
    33541 ttcgggtggt aattgtgctc acacaccaca gccatgactt tttcaacggt tttgtcgctc
    33601 acacggtatt gcttcgcttt gccgttaata acatagcttg cagtggtccg cgacactccc
    33661 gccagccgag cgatttcatc cagtttcaca attgcccctt gcgtaaaatg taaaaaccat
    33721 agccattgta ctggtatggg ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg
    33781 caactgcttt tattccaggt tcccactgat ttcgcaaaaa aagcccaacg tgacatgttg
    33841 ggctgcaaat tgcgcactga gatcattctc aacgcattat tttatcgccg cgcgaaagtc
    33901 cgaccacacc agagcgagca acttccacaa ttttcgccac atcgcgaatc gatgctaaaa
    33961 atgcatcaag cttatcgctg gtgcctgcta attgaacggt ataaagcgag ggggtgacat
    34021 cgataatttg cccacggaat atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc
    34081 tggcctgaat tttcaccagc atgatttccc gctcaacatg cgcgccctgc cccaactcac
    34141 tcacgcgcaa gacatcgacc agcttgtgta attgcttttc gatctgctca agcacttttt
    34201 catcgcccac ggtctggatg gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg
    34261 tcaggctttc aatgttgtag ccacgctggg aaaaaaggcc aatcacgcgg gataacgcgc
    34321 ctgattcatt ttcgagtaag actgataata tccggcgcat aatcaggttc tctccgtttt
    34381 gcttaaccac atttcatcca ttccgccccc gcgaatctgc atcgggtaga cgtgctcact
    34441 gccatcgacg gtaacatcaa caaacaccag gcgattattg cgcacctgtt ccagcgcctc
    34501 gctaagtttg ctttccagct catgcggatg agagatctgg atcccgacgt gaccataggc
    34561 ttccgccaga cggacgaaat cgggtagtga ttgcatataa gattgtgaat gacggccgga
    34621 atagatcatg tcctgccact gtttcaccat ccccagatag cggttattga gattcaccac
    34681 cagtaccggc aactcgtatt gcaacgcggt agacagttcc tggatgttca tctgaatact
    34741 gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac gccattttga cgcccagtgc
    34801 cgcaggtaaa ccaaaaccca tcgtgccgag gccaccggaa ttgatccagc gacgcggttt
    34861 gtcgaatgga taataaagtg cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta
    34921 agcgtctccc ttcgtcaacc gccaaagagt ctcgatcacc gcctgcggtt taatcttttc
    34981 actgtgagtg tcatatttca ggcactgacg agcgcgccac tgttcaattt gctgccacca
    35041 gtcgcggatc tcatccagtg gttgatgggc ggattcttgc gacaagagtt caagcatttg
    35101 ttcgaggacc tggcgagcat cccccacaat cgggatatcc gcagtcacgg ttttagaaat
    35161 ggaagtagga tcaatatcga tatgcagaac agtggcattt gggcagtact ttgccagatt
    35221 gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa atcacatccg cgttatgcat
    35281 cgtcatattg gcttcgtagg taccgtgcat tcccagcatg cccagcgcct gacgatgcgt
    35341 tgccggaaac gcccccagcc ccatcaatga ggaaacaacg ggcagattca acgcctccac
    35401 cgtttctttc aactgctgat ggcagcccgc catgattgcc ccaccgccta cgtagacaac
    35461 cggttttttt gccgctacca gcgtttgcag agcgcgctta atttgccctt tatgtccggt
    35521 agtagtggga ttgtaagaac gcatactgac cgactcaggc cagacatagg gtaatttgtt
    35581 cgccggatta agaatatctt tcggtaaatc aacgaccact ggtcctgggc gaccacttgc
    35641 cgccagccag aaagcctttt tcagcacctg cggaatgtct tccgtttgct taaccagaaa
    35701 actgtgttta accaccggtc gcgaaatccc caccatgtcg cactcctgaa aggcatcgta
    35761 acctatcaac gaggtcgcta cctgcccgga aaggacaact aatggaatgg aatccatata
    35821 agcggtggcg atgccggtaa tcgcattggt cgcccctgga cccgacgtta ccagcacgac
    35881 gccgacttcc ccggtcgcgc gcgccagacc atcggccata tgcactgccg cctgctcatg
    35941 acgaactaaa acatgatcga taccacccac ggtatgcaat gcatcgtaaa tatcaaggac
    36001 tgcgcctccg ggataaccga atacttgttt aacgccctga tcgataagcg atcggacgac
    36061 catctcggct ccagacaaca tctccatggc ctgcctcact gtttgacgga aaaaatgtgt
    36121 aaaacacatt aaccgtccga tcttgcttaa gcaattggca gccagagaca taaacgcagg
    36181 gtaataagaa taaacaggag gaaaggtgaa aagaaacgat aaaatcctcc attacgaagg
    36241 attaaattga taataaatcg gctgaatccc acaacttaca gcaatttctc tattttttta
    36301 attaatccta aaacagagag cccacacatt cagaataaaa ttctacattc aataaaaaat
    36361 acagaataaa ccagacattc atgcctgacc tattctgcat tcagttagcg tttgcaaatt
    36421 gagactaatt gctcttccat ccactgatgg cctttatcgc gcccggcagc ttcatgccag
    36481 gagagataac aggttctgct attttgtttt aacggcagcg gtaatacctg taattctaag
    36541 gattcagcga actcttcagc cagccaacgc ggcgcaatag cgaccaaatg cgtttgcgac
    36601 accacgctaa gtacgctcat cattgccatg ccctgatacg cgatactggc ttgcttatct
    36661 accgtgtcat accaaggttg actaaatgac gcgaaacgat cgagcgaaac cgccgcatgt
    36721 tgttcgttat aaacatcatg tttcagtaac gggcccttaa ttgttggatg atttttgctg
    36781 gctaccagca ccatttcatc tttaaataat ggcacgctgg taaattcagg acgatggaag
    36841 tcttcataac taatcacaaa ctccgtttcc tgataacgca gctgatgttc agtgttctga
    36901 tttaatgaag acttgaacat aacatgtata tttggcgcaa tctgctcaat gtgattataa
    36961 atctgcgagg tcagaatgct gtctaacggg ctgcaaacac aaagatgaaa tacacgttca
    37021 ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt gtactagttg caatgcctga
    37081 cgaactgagc caaaaagttg aaatgcgcga gcagtcggtt gaataccacg gccataacga
    37141 acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa cagcgttact gaccgcaggt
    37201 tgcgacatac ccagaacatg agcggcacgg gtaatgtttt gctcctgcat cacggcatcg
    37261 aaaacggtta ataagttgag atcgaccatg cgtagctgtg gcttgcttaa ctccgccgtc
    37321 tctgaatgat ctgtttgtac ctctggcata cttaactcca ctgtcacact taactccctt
    37381 tcccttattg gaatgcagag aaataatcct gaagaatatg atttatcatg catataaaat
    37441 aagaaaaagc aaaatgagta aaattcggaa aacataaaga tacgggttct gataaaaaca
    37501 gaatccattg ccgtggaatg agtcatttac cacagcataa taatccataa tgtttttgtt
    37561 gcgaaaacaa tctaatc
//
LOCUS       DAZFFI010000038        36968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFFI010000038 DAZFFI010000000
VERSION     DAZFFI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.038"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..730
                     /locus_tag="ACHJ12_002216"
     CDS             29..730
                     /locus_tag="ACHJ12_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4989995.1"
                     /translation="MKTCITKGIVTVSFTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            755..3355
                     /locus_tag="ACHJ12_002217"
     CDS             755..3355
                     /locus_tag="ACHJ12_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4989996.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3346..4416
                     /locus_tag="ACHJ12_002218"
     CDS             3346..4416
                     /locus_tag="ACHJ12_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4989997.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4428..4970
                     /locus_tag="ACHJ12_002219"
     CDS             4428..4970
                     /locus_tag="ACHJ12_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4989998.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4978..5493
                     /locus_tag="ACHJ12_002220"
     CDS             4978..5493
                     /locus_tag="ACHJ12_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4989999.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5459..6196
                     /locus_tag="ACHJ12_002221"
     CDS             5459..6196
                     /locus_tag="ACHJ12_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4990000.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6307..7317
                     /gene="pyrD"
                     /locus_tag="ACHJ12_002222"
     CDS             6307..7317
                     /gene="pyrD"
                     /locus_tag="ACHJ12_002222"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4990001.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7491..8033
                     /gene="zapC"
                     /locus_tag="ACHJ12_002223"
     CDS             7491..8033
                     /gene="zapC"
                     /locus_tag="ACHJ12_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4990002.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8030..9139)
                     /gene="ycbX"
                     /locus_tag="ACHJ12_002224"
     CDS             complement(8030..9139)
                     /gene="ycbX"
                     /locus_tag="ACHJ12_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4990003.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9383..11491
                     /gene="rlmKL"
                     /locus_tag="ACHJ12_002225"
     CDS             9383..11491
                     /gene="rlmKL"
                     /locus_tag="ACHJ12_002225"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4990004.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSATLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11502..13409
                     /locus_tag="ACHJ12_002226"
     CDS             11502..13409
                     /locus_tag="ACHJ12_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4990005.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13539..14792
                     /gene="pqiA"
                     /locus_tag="ACHJ12_002227"
     CDS             13539..14792
                     /gene="pqiA"
                     /locus_tag="ACHJ12_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4990006.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14797..16437
                     /gene="pqiB"
                     /locus_tag="ACHJ12_002228"
     CDS             14797..16437
                     /gene="pqiB"
                     /locus_tag="ACHJ12_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4990007.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSVAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16434..16997
                     /gene="pqiC"
                     /locus_tag="ACHJ12_002229"
     CDS             16434..16997
                     /gene="pqiC"
                     /locus_tag="ACHJ12_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4990008.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17253..17420
                     /gene="rmf"
                     /locus_tag="ACHJ12_002230"
     CDS             17253..17420
                     /gene="rmf"
                     /locus_tag="ACHJ12_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4990009.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17490..18008)
                     /gene="fabA"
                     /locus_tag="ACHJ12_002231"
     CDS             complement(17490..18008)
                     /gene="fabA"
                     /locus_tag="ACHJ12_002231"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4990010.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18077..19837)
                     /locus_tag="ACHJ12_002232"
     CDS             complement(18077..19837)
                     /locus_tag="ACHJ12_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4990011.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20023..20475
                     /gene="matP"
                     /locus_tag="ACHJ12_002233"
     CDS             20023..20475
                     /gene="matP"
                     /locus_tag="ACHJ12_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4990012.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20551..21591)
                     /gene="ompA"
                     /locus_tag="ACHJ12_002234"
     CDS             complement(20551..21591)
                     /gene="ompA"
                     /locus_tag="ACHJ12_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4990013.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21948..22457)
                     /gene="sulA"
                     /locus_tag="ACHJ12_002235"
     CDS             complement(21948..22457)
                     /gene="sulA"
                     /locus_tag="ACHJ12_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4990014.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22676..23305
                     /gene="sxy"
                     /locus_tag="ACHJ12_002236"
     CDS             22676..23305
                     /gene="sxy"
                     /locus_tag="ACHJ12_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4990015.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23268..25430)
                     /gene="yccS"
                     /locus_tag="ACHJ12_002237"
     CDS             complement(23268..25430)
                     /gene="yccS"
                     /locus_tag="ACHJ12_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4990016.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25440..25886)
                     /locus_tag="ACHJ12_002238"
     CDS             complement(25440..25886)
                     /locus_tag="ACHJ12_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4990017.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26009..28063
                     /gene="helD"
                     /locus_tag="ACHJ12_002239"
     CDS             26009..28063
                     /gene="helD"
                     /locus_tag="ACHJ12_002239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4990018.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28095..28553)
                     /gene="mgsA"
                     /locus_tag="ACHJ12_002240"
     CDS             complement(28095..28553)
                     /gene="mgsA"
                     /locus_tag="ACHJ12_002240"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4990019.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28649..29311)
                     /locus_tag="ACHJ12_002241"
     CDS             complement(28649..29311)
                     /locus_tag="ACHJ12_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4990020.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29484..29897
                     /locus_tag="ACHJ12_002242"
     CDS             29484..29897
                     /locus_tag="ACHJ12_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4990021.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29942..30259)
                     /gene="hspQ"
                     /locus_tag="ACHJ12_002243"
     CDS             complement(29942..30259)
                     /gene="hspQ"
                     /locus_tag="ACHJ12_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4990022.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30317..31507)
                     /gene="rlmI"
                     /locus_tag="ACHJ12_002244"
     CDS             complement(30317..31507)
                     /gene="rlmI"
                     /locus_tag="ACHJ12_002244"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4990023.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31602..31880
                     /gene="yccX"
                     /locus_tag="ACHJ12_002245"
     CDS             31602..31880
                     /gene="yccX"
                     /locus_tag="ACHJ12_002245"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4990024.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(31877..32206)
                     /gene="tusE"
                     /locus_tag="ACHJ12_002246"
     CDS             complement(31877..32206)
                     /gene="tusE"
                     /locus_tag="ACHJ12_002246"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4990025.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32297..32956)
                     /gene="yccA"
                     /locus_tag="ACHJ12_002247"
     CDS             complement(32297..32956)
                     /gene="yccA"
                     /locus_tag="ACHJ12_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4990026.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33364..34383)
                     /locus_tag="ACHJ12_002248"
     CDS             complement(33364..34383)
                     /locus_tag="ACHJ12_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4990027.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34361..34603)
                     /locus_tag="ACHJ12_002249"
     CDS             complement(34361..34603)
                     /locus_tag="ACHJ12_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4990028.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(34671..>36968)
                     /locus_tag="ACHJ12_002250"
     CDS             complement(34671..>36968)
                     /locus_tag="ACHJ12_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4990029.1"
                     /translation="EAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDG
                     ITMIFIAPSPSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKP
                     VTHVSREELQALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPV
                     NSSLGLMTTFFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPD
                     LVHTLDTLDVEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGI
                     LDYSRAAIFALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDI
                     LEEINGGLATECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKS
                     QVKVTAEEVNKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQ
                     NQQETEQNDQKAEQNSQNALQDEPETKQSEPVAQQQQEEEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPL
                     IAVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADF"
BASE COUNT         9355 a         8838 c         9572 g         9203 t
ORIGIN      
        1 tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac
       61 cgtaagtttt acggcaatat tactcagctg ctcatccgca tgggcggcag gtaaaggcgg
      121 gattggactt gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa tttcactggg
      181 tgtacgtaac accagtccgg atgttcctta tcttattcag tcatgggtga tgaccccaga
      241 taataaaaaa tcagcagatt ttattattac accaccgcta tttgtgctga atccggctaa
      301 tgagaatctg ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct
      361 tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagagg gaaataccct
      421 gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc
      481 cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag
      541 taacccaaca ccttatttca ttaccctgac agacctgaaa ataggtggaa aagtagttaa
      601 aaatcaaatg atttccccct ttgataaata ccaattttct ctgccaaagg gggccaaaaa
      661 tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg
      721 taaatcgtaa gccgtcttca gttaagagag cgagatgtat agaactcatc gacaacacag
      781 cctgttaagc tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc
      841 agcgttcaat gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc
      901 aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg
      961 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
     1021 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt
     1081 taatactgat gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc
     1141 ggagattatt ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc
     1201 agtaccgcaa atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga
     1261 tgaagggatc aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag
     1321 cagcgcagac agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt
     1381 aggcccatgg agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga
     1441 atggaagaat ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac
     1501 cgttggtgat gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca
     1561 gctggcgtca gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg
     1621 tggtatcgcc aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca
     1681 aacttatgta tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg
     1741 tgatttgtta gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt
     1801 ttccagcgtg ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa
     1861 atacagaacc aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg
     1921 gggcggaccg tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc
     1981 cgccatgttt ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac
     2041 ccaggcgaag agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct
     2101 gtatgccaaa acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc
     2161 gacgtcgggt ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt
     2221 taatgacggt gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac
     2281 caaacgtggc aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta
     2341 tttaagtggt agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt
     2401 tggctacaac acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc
     2461 ccgtggtcaa cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct
     2521 gttgctcccc agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc
     2581 taacaccagt atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact
     2641 actcgatgac ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac
     2701 ggctaatggt agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta
     2761 caactacagc gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt
     2821 tgcccattca cagggcatta ccctggggca atcgctgggg gaaactaacg tgctgattgc
     2881 agcaccaggc gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg
     2941 cggatatacc gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc
     3001 ggcatcgtta aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa
     3061 aggggcgttg gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac
     3121 atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac
     3181 taatagcggc attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg
     3241 agcgataact gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct
     3301 taccgaacaa caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt
     3361 ggagaaaaat gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc
     3421 gctcaaactg ctgcttatag cgggcagtgt cataccactc aggggaatcc gtatattggc
     3481 gtcaattttg gcgttaaaac cctggaggaa gaagaaaata cggctggagt agttaaagac
     3541 aaattttatc agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac
     3601 aatgtcagaa gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac
     3661 aactggtaca aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt
     3721 tctcctactg cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt
     3781 tgcgatcccg gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt
     3841 tccctgaaaa tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg
     3901 cgattatttg aatgctacaa catacccgca ggtgattcct gcacgactac aggtacaccg
     3961 gttttagtgt attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc
     4021 ggagagacta tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg
     4081 cataaacccc ttggggccag aacggcagaa ctcgcaattc cagtcaggtg taacacggga
     4141 aacgcggggt tagttaacgt caacctgagt ctgacggcaa ccacagaccc cagctatccc
     4201 caggcgatta agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac
     4261 attatttccc ctgctggtgg aacattaccg ctctctattc ctgatgatgc agacagtatc
     4321 gcgcgaatga atgtctatcc agtcagcacg acaggtgtac caccagaaac cgggcgattt
     4381 gaagccacgg caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa
     4441 cgatatttca gtgcgttatc ttgttcttta gcattcttaa catccatgtc gggatggctg
     4501 ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg
     4561 ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg
     4621 ccgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg
     4681 gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc
     4741 tcaaactaac tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc
     4801 ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa
     4861 aagccgggga taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa
     4921 cgggcggtaa tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg
     4981 ttgaaacgga taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt
     5041 gcccatgatg gcacagtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca
     5101 caggatagca agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat
     5161 gggaattatg gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc
     5221 agcacggttg acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc
     5281 attgaaagtg gtacggatgc agccagcggg ctggcaattg cgattctgga tgatgcgaag
     5341 atattgattc cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca
     5401 ttaacttttt atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat
     5461 gccagcgcga catttgtact tcattatgac taatacgtgg aacagattgg cgctcttgat
     5521 tttcgccgtt ttatcgctgc tggtggcggg tgaattgcgg gcaggagtgg tggtcggcgg
     5581 aacgcgattt atctttccgg cagacagaga atcgatatct attttactga ctaataccag
     5641 tcaggaatcc tggcttatta acagtaaaat caaccgccca acgcgttggg ccggggggga
     5701 agcgtcgaca gtgccagcac cattactggc cgctccgcca cttattctcc tgaagcccgg
     5761 tactacaggc acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga
     5821 aacgctattt gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt
     5881 aaaagtggcg atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga
     5941 cccgctggaa gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac
     6001 taacccaacg ccttactaca ttaacctgat tcagttgagc gtaaatggta aagcgttaag
     6061 taatgcggga gttgtgccgc ctaaaagcca gcgtcagaca agctggtgtc aggcaatcgc
     6121 accgtgtcat gtcgcctggc gggcgataaa tgattacggc gggttgagtg caaagaagga
     6181 gcaaaatctg ccctgaaaca ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat
     6241 tcccttttgg tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga
     6301 gagttcatgt actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct
     6361 catgagttta cttttcagca attacgccgt attacaggaa cgccgtttga agcactggtg
     6421 cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt
     6481 ggtctggcag ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcgatggga
     6541 tttggatcga tcgagatcgg taccgtcacg ccacgtccac agccaggtaa tgacaagccg
     6601 cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt
     6661 ggcgttgata acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cctgggtatt
     6721 aacatcggca aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt
     6781 atggaaaaaa tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc
     6841 ccaggattac gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa
     6901 aataagcaaa atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc
     6961 gcgccggatc tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat
     7021 attgatggcg ttattgcaac caataccaca ctcgatcgtt ctcttgttca gggaatgaaa
     7081 aattgcgatc aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag caccgaaatt
     7141 attcgccgct tgtcactgga attaaacggt cgcttaccga tcatcggtgt tggcggcatc
     7201 gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat
     7261 tctggtttta tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta
     7321 tttcttcgaa ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta
     7381 tattcatcgc tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga
     7441 agcgacaatg gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta
     7501 aaccagacga taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt
     7561 tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa gatgttgact ccagatgcct
     7621 tttctcccgc aggcttttgc gttgacgacg ccgcgctcta tttctctttt gaagaaaagt
     7681 gccgcgattt taatttatct aaagaacaaa aagccgaact ggtgctgaat gcactggtag
     7741 caattcgcta tctcaaaccg caaatgccga aaagctggca ttttgtttcc catggtgaaa
     7801 tgtgggtgcc catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg
     7861 tcaatttgct ggtcgttgaa tctggcgaaa atgccgcact atgcctgctg gcacaaccct
     7921 gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca
     7981 ggctgaaacc gcaggtcaat gttgacagct tcagcctcga acaggcagtc taacgcgcca
     8041 acttaagtgc agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg
     8101 ctgatttttt tagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa
     8161 tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca
     8221 cctgttggtt atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg
     8281 gttgttgcgt gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag
     8341 ccgttgccag aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta
     8401 aattctgacc aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt
     8461 ttaatggttc gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac
     8521 agcggctaca aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc
     8581 tatcttcttc ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt
     8641 ttacactggc cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt
     8701 aaggatagcc atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca
     8761 tttgtggccc cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt
     8821 tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt
     8881 cttgtgtggc gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat
     8941 gcaagccatc atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg
     9001 taataaacgt accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga
     9061 catcagccag agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa
     9121 gccgggttaa tgtcaccacg gtgcggtcct caaatgaaaa taagccctca actttatgac
     9181 atgacgcgct tattagctat aatgcgcaac aattttctta gcgcctgtcc cattaggcta
     9241 ttttatttgt cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc
     9301 cggtttctcc gcgctgtcca tgtccagact gactgcaaca attacgccta ctgcggtagg
     9361 ctcttagagt aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga
     9421 agagctgtta aaaactgaac tggaaaacct gggggccgtt gaatgccagg tggttcaggg
     9481 tggggtccat ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct
     9541 ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta
     9601 tctcggtgtt caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt
     9661 ccacttcagt ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa
     9721 agacgcgatc gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga
     9781 tgcgccggat atccgcgtta acgtctggct gcataaagaa accgccagta tcgcccttga
     9841 tctcagtggt gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat
     9901 caaagaaacc ctggcagccg ctattgtgat gcgatccggc tggcagccag gaacaccgct
     9961 gctcgatccg atgtgtggtt ccgctacgtt gctgattgaa gcggcgatgc tggcgaccga
    10021 tcgcgcacca ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga
    10081 agctatctgg caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctggctga
    10141 gtatagctct catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcacgcac
    10201 taacgcccgt cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca
    10261 actgaccaat ccgctgccga aagggccgta cggcacagtg ttgagcaacc cgccatacgg
    10321 ggaacgtctg gacagcgaac cggcgctgat tgcgctgcat agcctgctgg gtcggatcat
    10381 gaaaaaccag tttggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag
    10441 ctgcttgcag ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt
    10501 acagaaaaat taccacgttg ccgaaagcac tccagacagc aaaccggcga tggtagcgga
    10561 agactacgcc aaccgcctgc gtaagaacct caaaaaattc gagaagtggg ctcgccagga
    10621 aggaattgaa tgttaccgcc tgtatgacgc cgatctaccg gaatataacg ttgccgttga
    10681 ccgttatgcc gactgggtgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgctca
    10741 caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc
    10801 gccaaacaaa ctggtgctga aaacccgtga acgccagaag ggcaaaaatc agtaccagaa
    10861 actgggcgag aagggcgagt ttcttgaagt taccgaatat aacgctcact tgttggtgaa
    10921 cctgacggat tacctcgata ccggtctgtt tctcgatcac cgtatcgccc gtcgtatgct
    10981 cggtcagatg agcaaaggca aagatttcct caacctgttc tcttataccg gcagcgccac
    11041 cgtgcatgcg ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta
    11101 tctggagtgg gcagaacgca acctgcgttt gaatggcctg accgggcgtg cgcatcgcct
    11161 gattcaggcc gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt
    11221 tatcgatccg ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg
    11281 cgatcatctg gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgattat
    11341 gttctcgaac aataaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact
    11401 gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat
    11461 ccacaactgc tggctgatta ccgcagccta aaggaatagt aatgtcatta atcagtatgc
    11521 atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg
    11581 aagataacga acgtgtttgt ctggtgggcc gcaacggcgc gggcaaatcg acgttaatga
    11641 aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat tatttacgag caagatttga
    11701 ttgtagctcg tctgcaacag gatccgccgc gtaacgttga gggtagcgtt tatgatttcg
    11761 ttgccgaagg cattgaagaa caagcggaat atctgaaacg ctatcacgat atttcgcgcc
    11821 tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt caggaacagc
    11881 tggatcacca caacctgtgg cagctggaaa accgcatcaa cgaagtgctg gcgcaactgg
    11941 ggttagatcc taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat
    12001 taggacgcgc gctggtgagt aatccgcgcg tgctgttgct tgatgaaccg acaaaccacc
    12061 tggatattga aaccatcgac tggctggaag ggtttttgaa aactttcaac gggacgatta
    12121 ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt gttgatctcg
    12181 atcgcggcaa gctggtgacc tatccaggga attacgacca gtacctgctg gaaaaagaag
    12241 aagccctgcg cgtggaagaa ttacaaaatg ccgagttcga tcgcaaactg gcgcaggaag
    12301 aggtgtggat ccgccagggg atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg
    12361 ccctgaaagc gatgcgtcgc gagcgtggtg aacgtcgcga agtgatgggt accgcaaaga
    12421 tgcaggtgga agaggccagc cgctccggta agatcgtttt cgaaatggaa gacgtttgct
    12481 accaggttga cggtaagcaa ctggtgaaag atttttcggc ccaggttcta cgtggcgaca
    12541 aaattgccct gattggtccg aatgggtgcg gcaaaaccac gctgctgaaa ctgatgctcg
    12601 gtcagcttca agcggacagc gggcgtattc acgttggcac caaactggaa gtggcttatt
    12661 tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt gatggataac cttgccgaag
    12721 gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt attgggctat ttgcaggact
    12781 ttctgttcca tccgaaacgg gcgatgacgc cggtacgtgc gctttctggc ggtgagcgga
    12841 accgcttgct gctggcgcgt ttgttcctca aaccaagcaa cttattgatt cttgacgaac
    12901 cgaccaacga tcttgatgtc gaaacgctgg aactgctgga agaactgatc gacagctatc
    12961 agggcacggt attgctggtt agccacgatc gtcagtttgt cgataacacc gttacagaat
    13021 gttggatctt cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat catgatgccc
    13081 gtggtcagca agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa accgaagaag
    13141 ccgccgcggc aaaagcagaa actgtaaaac gcagcagtag caaactaagc tataaattgc
    13201 agcgcgaact ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc
    13261 tacagacgca agtggcggat gcttccttct tcagtcagcc gcatgagcag acgcaaaaag
    13321 tgcttgctga tatggctgct gcagagcagg agctggagca agcctttgaa cgctgggagt
    13381 atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa cagagacagc
    13441 tgacaacggt aaaattgcct gatgaactgg gtttatcagg catgaaaacc aacactttgt
    13501 tagtcatctg taaaatagcg catcattaag gagtaccaat gtgcgaacat catcatgccg
    13561 cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg
    13621 gtcagaaagc ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg gatgcccctc
    13681 ggcagcgtcc caccgcctat gcgttggctg cactgttcat gctgttgctg tccaacttgt
    13741 ttccttttgt gaatatgaac gttgcaggag ttaccagtga aattacatta ctggaaattc
    13801 ccggcgtgct tttttctgag gactacgcca gcctcggcac ctttttcctg ttgtttgtgc
    13861 aactggttcc cgcgttttgt ctgataacca ttctgttact ggtgaatcgc gcggaattac
    13921 cggtccgttt aaaagagcaa ctggcacggg tgctttttca actcaaaacc tggggaatgg
    13981 cggagatttt cctcgctggt gtgctggtca gtttcgttaa actgatggct tacggcagca
    14041 ttggggtagg cagcagcttt ctcccctggt gtttattttg tgtcctgcaa ctgcgcgctt
    14101 ttcagtgcgt tgatcgtcgc tggttatggg acgacatcgc cccgatgcca gaactgcgcc
    14161 agccgctaaa accaggcgtc acggggatac gtcaggggct gcgttcgtgc tcctgttgta
    14221 cggcaatcct tcctgctgat gaacccgtgt gcccgcgttg tagtaccaaa gggtacgttc
    14281 ggcgtagaaa cagcctgcag tggacactcg cgctgcttgt aacgtccatc atgctgtatc
    14341 ttccggctaa tattttgccc atcatggtga cggatttatt aggctcgaag atgccgtcga
    14401 cgattctcgc tggggtcatt ctgttatgga gcgaaggctc ttatcccgtc gctgcggtga
    14461 tctttctggc cagtattatg gtgccaacgt taaagatgat cgccatcgcg tggctgtgtt
    14521 gggatgccaa agggcatggc aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg
    14581 ttgagtttgt aggccgctgg tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc
    14641 tggtgcgtat gggaggttta atgagtattt atccggcaat gggtgcatta atgtttgctt
    14701 tagtcgtcat aatgacaatg ttttctgcta tgacgtttga cccgcgtttg tcgtgggatc
    14761 gtcaacctga atcagagcat gaggagtcct gacagtatgg aatctaataa tggggaagcc
    14821 aaaatccaga aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt cacggcgctc
    14881 attggggcct gggttctttt ttatcattac agccatcagg gaccggaagt gaccctgatc
    14941 accgcgaatg cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg tagcgttgac
    15001 gtcggcgtgg ttgaaagcgc cacactggct gatgatttga cgcacgttga aatcaaagcg
    15061 cggctgaatt ccggtatgga aaaattgctg cataaagaca ccgtcttttg ggtggtgaaa
    15121 ccgcagattg gtcgcgaagg gattagcggc ctgggaacgc tgctgtctgg agtttatatc
    15181 gaactgcagc caggcgcgaa aggcagcaaa atggataaat acgatttgct ggactcgcca
    15241 ccgttggccc cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa aaaagccggg
    15301 cagctctcgc caggagatcc ggtgctgttc cgtggctatc gggtaggttc ggttgaaacc
    15361 agcaccttcg atacacaaaa acgcaatatc agctatcaac tgttcatcaa tgcaccttat
    15421 gaccgactgg tgaccaacaa tgttcgcttc tggaaagata gtggcattgc ggttgatctg
    15481 acgtcagcag ggatgcgtgt ggagatgggc tcattgacaa cgctgctgag tggcggtgtc
    15541 agctttgatg tgccggaagg tctggattta gggcagccag tggcaccgaa aacagctttc
    15601 gttttgtatg atgatcagaa gagcattcag gattcgttgt acaccgatca cattgattat
    15661 ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt agagttccgg
    15721 ggtattcgcc tgggtaccgt aagcaaagtg ccattctttg cgccgaatat gcgtcagaca
    15781 tttaacgatg attaccgtat tccggtactg attcgtatcg agccagagcg gctgaaaatg
    15841 cagcttggcg aaaatgcgga tgttgttgag caccttggcg aattgttgaa acgtggttta
    15901 cgcggatcgc tgaaaaccgg aaacctggtc actggtgcac tgtatgttga tctcgatttc
    15961 tatccaaata cgcctgcaat aaccggtatt cgtgaattta atggttatca gattatcccg
    16021 accgttagcg gcggcctggc gcaaatccag caacgactga tggaagcgtt ggataagatc
    16081 aacaaactgc cattgaatcc gatgattgaa caggcaacca gtacgctttc tgaaagtcag
    16141 cgcacaatga aaaacctgca aacgacgctg gatagcatga acaagatcct cgctagccag
    16201 tcgatgcagc agttgccgac ggatatgcag tcaacgttgc gtgaattgaa tcgcagcatg
    16261 cagggcttcc agcctggctc cgtagcctac aacaagatgg tggcggatat gcagcgcctt
    16321 gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa gagtaacgcg
    16381 ctggtatttg aagcgaagga caaaaaagat ccagagccga agagggcgaa acaatgaaaa
    16441 agtggctagt gacgattgca gcactgtggc tggtcggatg cagctccggc gaaattaata
    16501 aaaactatta ccagttacct gtggtgcaga gcggtacaca aagtaccgcc agccagggca
    16561 atcgtctgtt atgggtagag caggtcactg ttcctgacta tctggcgggg aatggtgtgg
    16621 tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg gccagcccgt
    16681 tggatcaaca gttgcgcaac accctggttg ccaacctgag tacgcaactg cccggctggg
    16741 tggttgcctc ccagcctctg ggaagcgccc aggacacgct caatgttacc gtaacggagt
    16801 ttaacggtcg ctatgatggc aaggtcattg tcagtggtga gtggctgttg aaccaccagg
    16861 gacaactgat caaacgtccg ttccgtctgg aaggagtgca aactcaggat ggttacgatg
    16921 agatggttaa agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt gcacaagaga
    16981 taaagcgtct accttaatta taaagatttg taaatataac cgtctccggt atgttgcctg
    17041 aggcggtttt tttgtttcta acgtgcggaa aaatttgttc ctcttcacat tttttgtaca
    17101 accgacatgc ccgtgtagct cacaaatatg acagtggcgt gaattttgcg cattgacggc
    17161 agttatgatt cgcggtattg cttaactgtg attgcacatt tagtaatcac tgttttcttt
    17221 tccaccagaa accagtatga gggaaacgag gcatgaagag acaaaaacga gatcgcctgg
    17281 aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc
    17341 cctatcagac gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa gccatggcgg
    17401 acagggtagt aatggcctga ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt
    17461 tcgccttttg atactctgtc tgattataat cagaaggcag acgtatcctg gaacagaccg
    17521 actttcaggt cgctggcggt atagataaga cgaccatcaa ccagcacttc gccatccgcc
    17581 aggcccataa tcagacgacg gttaacaatg cgtttaaagt gaatacggta ggtcactttt
    17641 ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc caacgcccag cgcgcggcct
    17701 ttaccttcgc cgcccagcca gccgaggtag aaccctacca gctgccacat tgcgtccagg
    17761 cccaggcatc ccggcataac cggatcgcca ataaagtggc atccgaagaa ccacagatcc
    17821 ggattgatat ccagttctgc ttcaacatac cctttgtcga agttaccacc cgtttcggtc
    17881 attttgacca cacggtccat catcagcatg ttcggtgctg gcaattgcgg gcctttagcg
    17941 ccaaacagtt caccgcgacc agaggcaaga aggtcttctt ttgtatagga ttcgcgttta
    18001 tctaccatgt tctctgtaag ccttatttta ttgaagcacg caggatagct aacacgtgta
    18061 cgctgaacaa gtccgatcag ttcggaataa accagttcag ccaacgtaat ggccatggaa
    18121 aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca
    18181 gcgtcgtttg gccttcgcca tcccacacca gatttaataa taacggcagt gcgtcagtca
    18241 catcgtctac tgcccagatg gtgaatttgc cttcttctac cgctttcacc agttcactgt
    18301 gaagacttaa atggcgaacg ttagctgtgg ggataatgac accttgtttc ccggttaact
    18361 cacgttgctg gcaaatagca aagaagcctt cgattttctc atttaaacca ccgaccggct
    18421 gggcgcgacc gaactgatcg actgaacctg tgatagcgat actctggttc accggcacat
    18481 cggcgagggc gcttatcagg gcgcagagtt cagccatcga ggcactatct ccatcaactt
    18541 cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta
    18601 gttccgacat caggaacgct tgcatgatca tcatcccttt cgcatggata ttgccgccaa
    18661 gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca acgcagctaa
    18721 tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt
    18781 taatttgccc gatgcgttcg ccttcggttt caatcaggat ttgctcctga aggatctcat
    18841 cctgcatacg ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc attaagttta
    18901 gctgctcgcc ggagaaggtg tcgccatcac acagggaggc gacctcttta cactggcgga
    18961 ggatccactg cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt gctgcttcgc
    19021 ggataagtac cggccaggca tccgctcccg gtgcaggtaa gtgattatgt ctggcggtaa
    19081 atgtcaccca gcgacaccac tgggttactg actctgcatc gacaatctgc agagtatctt
    19141 caaattcgct ataaatagcc tgctctgaaa gctctggctc catctcctgg aaatcagcca
    19201 atgattcgcg ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa ggcacagaga
    19261 cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat
    19321 ttttcagccg catccacagc agaggttgcg ccagcagtgt acgcaaagag atgatgagaa
    19381 taccgccatt tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc
    19441 gcaggcagcc aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag
    19501 tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg gatggagtgg ccggaaacct
    19561 catagtgacc gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg gcattggcaa
    19621 taagattcag atactcagac tcttccgggg ccttcgccag cataaaagag gaggatgctc
    19681 gcgtatgcag caactgctcc agtgcgaatt gcagtcgcgg ttgagtatca ctgaataaag
    19741 gatcgttttc gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag
    19801 gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg
    19861 tcaaagccgc gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac
    19921 tcctggcatg ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag
    19981 ctgatattct gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt
    20041 gaaaatcttg aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag
    20101 ttaatcaccc gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg
    20161 ctcgaaaatg aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg
    20221 gtcaatcgca tgaagcaaac cattcgggca agacgaaagc gccattttaa tgcggaacac
    20281 cagcatacgc gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt
    20341 cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg
    20401 gaaaacaaag agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg
    20461 ctgggtaagg aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc
    20521 agcagcgggg tttttctacc agacgagaac ttaagcttgc ggctgagtta caacgtcttt
    20581 gataccttta acttcgatct ctacgcgacg atccggagcc aggcagtcga tcagtgcagc
    20641 acgctgtttc acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg
    20701 tgcggagatc ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc
    20761 acggcgctcg gacagaccct ggttgtaagc gtcagaaccg atgcggtcgg tgtaacccag
    20821 aacaactacg gaaccgtctt tcggatccag gttgctcagc tggctgtaca gctgatccag
    20881 agcagcctga ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt
    20941 cagagtgaag tgcttggtct gtacttccgg tgccggagct ggagccggag caactactgg
    21001 agctgcttcg ccctgaccga aacggtagga aacacccagg ctcagcatgc cgttgtccgg
    21061 acgagtgccg atggtgtgtg cgtcaccgat gttgttggtc cactggtatt ccagacgggt
    21121 agcgatttca ggagtgatcg cgtactcaac accgccagcg aagaccggag aaacgccggt
    21181 gtcgtggttt ttaccataaa cgttggattt agtgtctgca cgccatacca taccacccag
    21241 acgagtgtag atgtccaggt cgtcagtgat tgggtaaccc agtttagcgg tcagttgaac
    21301 gccctgagct ttgtatgcac cgttttcaac gctgcctttg tacggcatac gacctaacca
    21361 gtcgtaaccc atttcaaagc caacatacgg gttaacctgg taaccaccaa aagcaccagc
    21421 gcccagttgg ttttcatggg tcgggccatt gttgttgatg aaaccagtgt catggtactg
    21481 ggaccagccc agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac
    21541 ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc
    21601 ctcgttatca tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt
    21661 caccggggga tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc
    21721 tggcgatgta aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata
    21781 tgaaaaaaaa gtcttgtata aggtatgttt aatctttttt gtcagcgaca atttacagaa
    21841 gaaaatcgcg gaaaccgctt cagacaagcc tccgcaagga aaattagtca cgactgaaag
    21901 cattggctgg gcgacaaaaa aagttccagg attaatccta aatttactta atgatacaaa
    21961 ttagagtgaa tttttagccc ggaaagttgt ctcgtggcgt gagaggatgc gcttaccgga
    22021 cgcataataa accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct
    22081 tcttcagtca aatcatctgc caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa
    22141 gcgcgaacca ttgactccac agtgtggcaa ggggagagct ggctaatctg cattactttc
    22201 gttaagggta gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac
    22261 cagagttgcc agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc
    22321 gtcatcatgg gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt
    22381 tccgtagaga cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca
    22441 tagcctgaag tgtacataat caatccagcc cctgtgagtt actgtatgga tgtacagtac
    22501 atccagtgac aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg
    22561 gtgacgggaa aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt
    22621 aacggatccg ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa
    22681 aagcctctcc tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat
    22741 tgaataccga tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat
    22801 ggtttctgat ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa
    22861 acatccgcct gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta
    22921 tcgggttgat gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc
    22981 tctcgatgca gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt
    23041 gcccaatatg tcttttcatc tggaagcgat tctcggggag gtggggatta aggatgtacg
    23101 ggcgttacgt atacttgggg caaaaatgtg ttggttgcga ctgcggcagc aaaacagtct
    23161 ggtgacagaa aagattctgt ttatgcttga aggtgccatt atcggcattc atgaagctgc
    23221 gctcccggtg gcacgccgcc aggagcttgc agaatgggct gactctctta cgccgaaaca
    23281 ggagtttcct gcggaacttg agtaatctgg cgttgcagac gaccaatctc aggcagtaat
    23341 gcaatcaata atccaacttg ttgtacgacc agaggttctt tgctgtctgc ccgtggttca
    23401 agttgctgca tccgctgttt caggctagct aatgcctcat tgacgcgttc ttcatcagca
    23461 ggttgatgat gtaacgcgtc atcaacatag caaactgcgt catcaagaaa cgccagaatt
    23521 tcaggattag ttaactgctc ccggtgagca ccgagggctg agatatagct ggtaaacgta
    23581 tggttaaggc acagcaaccg aaacgcggct tcgcgaattt gcggggtaac gttcggttcg
    23641 ctggacatat ttgataccac cgacgccagt tcagcatcac ggttgtgtgc atcgcggcgg
    23701 gcaatacgat acgccagacg gttatcacgc ccctgatggt attgctccag tatggcatcg
    23761 agataccggc agttggcctc tgtggcgcgt tcgagcattc gcggcagatt gcgaaactgc
    23821 cagtcaggcc agatgtagct cactgccgcc cacgcaatgg cacaaccaat cagcgtatcg
    23881 attacgcgag gtaacgctac ttcaaaacct tcacccagca agttaaaaca cagtagcacc
    23941 aaaagtgtga tgaacatcgt tgcatgggca tattgcacgt tacggaaggc aaaaaagagc
    24001 acgccggtaa taaccagtag caccagctgc ccttccagtg acggtacaaa ccacagcaca
    24061 ggaatgccaa tggcgatacc gaccagcgta ccaataatcc ttaacttcag gcggtggcgc
    24121 gtggcgttat agtttggctg gcagacaaac aaacttgtca gcaagatcca atacccgtga
    24181 tgcattccgg ttatctgaat gatggcgtag ccgaagcaca acaccagcga cattcttacc
    24241 gcatgacgga agagggcgga ttccggcgtg aagtgacggc taagacgcag ccagatatca
    24301 ctcaatccgt gcgggctatc atcagcgagc tcattttcgt cattattatg gggcagtgcc
    24361 tgggcctgtt ctgattcaat tgttgccagt tgggcatcaa tggcgcgtaa attgttcagc
    24421 aaaaatccca gtgttttaag taaatcggca ggcgcgccgt tatcgcgcat ccgctccagc
    24481 gcagcatcaa tatgcgtaaa agcgcgctca aaatgcggat catgttgata aggctgacgc
    24541 agcaaaatac agcgtgacag ttgctggcac gcctggccct gcatcgacat cagccgctga
    24601 aaacggaaca gcacgtcgct gtggcgaaaa tgttcacgca atgtttgata ctgaatatga
    24661 gaagagctgg cacgctcgtg aatatcctgt gcgacaaagt aataatgcag cgtgcgacgc
    24721 gttccccgtt gaccacgatc gccacgtaag cgggtcagca gcgagagttt cgtctgattc
    24781 aatgtcgcca tcagctgacc gttggcgaga gccaaatcgt acagcggtgc ctggctttca
    24841 tcttcaatat caggatcaaa catgcgcgac ttgagctcaa gataacgcgc cagttgttca
    24901 tagcaacgcg ccaggttgtc ctgcagcggg cggaccggga acagcagatg accaataagt
    24961 gtcaggacgt tgtaccagac ggcaccggcc agcagataca tcggctgctg ataccagtgc
    25021 tcatacagtg atgttcccaa catagtgtaa atggcgatca gcaatgcacc gaaggcaatt
    25081 gttgcatagc gttgacccag accgccgagc aaaatgaagc cgctggtaga gagcgttaag
    25141 ccaatcgcaa atagccaggg ccagggaaac agcaattcta ctgaggccga ggcgataaaa
    25201 aagcagaaca gcgtaatgat gaggttacgc aaacgtcccg ccagtcggtc atcgagatcg
    25261 gtcagcgccg ctgccaccat ccccagcgtt agcggaatcg tcagttttac atcacccagc
    25321 caccacggaa acgctgtggt tccacaaagc gcaataaaaa tacgcgcgta atacagccag
    25381 gcgctgttcc aggtatagcg tttgagcaaa ggacttagca taaaggccat attagtggat
    25441 tattcaaaac gacgacgtgc attggcttca cgcgcagctt gcgccgtttc taccgatacc
    25501 acgcgacgac caaccggcca tagtgcaata gcggcaattt taaagttcgc aatgccgaca
    25561 ggaatgccaa tgattgaaat acattgtgcg atgcccgttg caatgtgcat caggcataac
    25621 caccagccaa agaaaatcag ccagaaaata ttcaataccg taccgccagt attcagcagc
    25681 acatttttgc cagccgggtt cagttcatcg acatgaatag cttcattgcc ataaggcacc
    25741 agagacagtt tagtgatctc ccagcaggat cgtgtcagcg gtaaggtaaa aatcagcata
    25801 atactgacca gagtcgccaa cagccagccc agagtggtgg caaatccgcc aagcacaaag
    25861 ttcagaatgt tcaaaacggt acgcataaaa cctcgcttta ctgtggtttt cagtaatggg
    25921 cggcaattgt aacgtttttt tgggctggag cacgttttct ctgacggtta cactgataag
    25981 aaataatttc gtgtggatct acagagtcat ggaactgaaa gcgacaacgc ttggaaaacg
    26041 tctggcacag cacccttacg atcgggcggt gatcctcaat gccgggatta aagtctccgg
    26101 cgatcgccac gaatacctta ttcctttcaa tcaattactg gcgattcact gtaagcgcgg
    26161 tctggtatgg ggcgagctgg aatttgtact gccggacgaa aaagtggtgc gtctgcacgg
    26221 caccgaatgg ggcgagacgc agcgttttta ccatcatctt gatgctcact ggcagcggtg
    26281 gagtggcgag atgagcgaaa ttgcgtctgg tgttttacgc cagcaactgg atttgattgc
    26341 cacgcgcact ggagaaaata aatggctgac gcgtgagcaa acctctggcg ttcagcaaca
    26401 aatccgccag gctatgtcgg cgttgccgtt gccggttaac cgactggaag aattcgataa
    26461 ctgccgtgag gcgtggcgta aatgtcaggc ctggttgaaa gatattgaaa gcgctcggtt
    26521 gcagcataac caggcgtata ccgaagccat gcttaccgag tatgcggatt ttttccgcca
    26581 ggtcgagtct tcaccgctga atccggcgca ggcccgggca gtcgttaatg gcgagcattc
    26641 tctgttagtg ctggcaggtg caggaagcgg aaaaacgtcg gtgctggtgg cccgtgcagg
    26701 ctggttgctg gcgcgtggtg aagcgtcccc tgagcaaatt ttattgctgg cgtttggtcg
    26761 caaagccgct gaagagatgg acgagcggat tcgcgaacgg ctacataccg aagacattac
    26821 cgcacgcacg tttcatgcgc tggcgctgca tattattcag cagggcagca aaaaagttcc
    26881 gatagtcagc aaactggaaa atgataccgc tgcccgtcat gaactcttta ttgctgagtg
    26941 gcgcaagcaa tgcagcgaaa agaaagcgca ggcgaagggc tggcggcaat ggctgacgga
    27001 agaaatgcag tggtcagtgc cagaaggtaa cttctgggat gatgaaaaat tacagcgtcg
    27061 ccttgcctct cgcctcgatc gctgggtaag tctgatgcgg atgcacggtg gtgcacaggc
    27121 agaaatgatt gccagtgcac ccgaagagat tcgcgatctg ttcagtaaac gtatcaagtt
    27181 gatggccccg ttattaaaag cctggaaagg tgcgctgaag gcagaaaacg ctgtcgattt
    27241 ttcgggcctt attcatcagg cgattgtgat tctggagaaa ggtcgcttta tcagcccgtg
    27301 gaagcatatt ctggttgatg aatttcagga tatctcgccg cagcgggcag cgttgttagc
    27361 ggcattacgc aagcaaaaca gtcagacgac gttgttcgcc gttggtgatg actggcaggc
    27421 gatttaccga ttcagcggtg cgcaaatgtc gctcaccacc gctttccatg aaaactttgg
    27481 tgaaggcgat cgctgtgatt tagacacgac ttaccgtttt aacagtcgta tcggtgaggt
    27541 ggcaaaccgg tttattcagc agaacccagg ccagttgaaa aagccgctaa acagcttaac
    27601 caatggagac gaaaaagccg tcacgttatt ggatgagagt caacttgacg ctttgctgga
    27661 taagctctct ggttatgcca aaccggaaga gcgcattctg atcctggcgc gttaccatca
    27721 catgaggcct gccagcctgg aaaaagcggc aacacgctgg ccgaagttgc aaatcgactt
    27781 tatgaccatt catgccagca aagggcaaca ggcggattac gtcatcatcg ttggcttgca
    27841 ggagggaagt gatggttttc cggctgcggc gcgggagtcg attatggaag aggcgctact
    27901 gccaccggtt gaggatttcc cggacgctga agaacggcgg ttaatgtacg tggcgctgac
    27961 ccgggcacgc catcgggtat gggcactgtt taacaaagag aatccctctc cctttgtgga
    28021 aatactgaaa aatctggatg tgccggtggc gagaaaaccg taagaaacag gtggcgtttg
    28081 ccacctgtgc aatattactt cagacggtcc gcgagataac gctgataatc ggggatcaga
    28141 atatcgaccg cgtcgttgaa atgcggcgac tggattatga agtctgccgt tgccacgttg
    28201 gtggcgaccg gaatgttcca taccgtcgcc agacgcagca aggctttcac gtcaggatcg
    28261 tgcggcacgg catttagtgg atcccagaag aaaatcaata catcaatttt cccttctgag
    28321 atcaatgcgc caacctgctg gtcacccccc attgggccac tcaacatcgc gttgacgttc
    28381 atgccggttg cacgggaaat caagttaccg gtagtgcctg ttgcatacag tacgtgttgt
    28441 tccagtaacg gttgatgccg ttccacccag ctcatcagca tttgtttgca gtgatcgtgt
    28501 gccaccagcg caatatgttt ccgcgcaggt aaagtgcgag tcgtcagttc cataatgtac
    28561 atccgtagtt aactttccta cagattactg taagcactta tcgctgcaag ataaagaccg
    28621 aaaaagcctg cgcacaggca caaaaatctc aggaagatgg ttgtttttcc gcccactgca
    28681 ggaaagtatt tcgcgtttgt gggtcagcca gtttaaacca atacttcagc cgttgttctg
    28741 tgagcacctg agactgcggt ggaatagcat ccagctcgga aacacccgaa agcagcgtac
    28801 tgtcatctgc catcatcgtg gcaaattgcg gtagtgaagc gcgtttcgcg gatttgttat
    28861 agcgttcaac ctcgacctca taatcaatcg tttttgccgt tgaggtaatg gcgagaatat
    28921 ccagctttac cggaatcggc gtcgcatcgc catccaacaa ttcaaggcgc ggcgcggcat
    28981 caaaatggtt agcttcccgc tcattctcca ggcgaggcag gcgaaaattg acctggttga
    29041 tgagctgggt attaaaactg accaccagcg gtggggagat atacagccgt tcttcactat
    29101 tggaaagatg aatcgtcttc tcaacgcgaa acactaactg atgcggtcca ttatccagtt
    29161 caatgctgtc cgcaccgcgc agcagagagc tggaaacctt cttgccgtcg agtaccagaa
    29221 gatcgacatc ggttgaaagc cgcagcgtgg tggcaaaaac ggataccggc aaacataatg
    29281 caatcaaggt ggtcacgatg ccggttttca taggtagctc ctgtcaaaag accgcccgaa
    29341 taataatgtg tcaaaatttt tctttaatca tacttactaa catatagaca tatttcccgc
    29401 ttttgctctc attcattcgt attagctgca tggttggcat gtcggctttg tcgtacactt
    29461 tgttaaacag tctggaggaa ataatgaaag aaaccgatat tgcaggcatt ttaacgtcca
    29521 ctcacaccat tgcgctggtg ggggcaagcg acaaacccga tcgtcccagc tatcgcgtga
    29581 tgaaatatct actcgaccag gggtatcacg tcattccggt ttcgccaaaa gttgccggca
    29641 aaacgctgct gggccagcag gggtatggca cgctggcaga cgttccggaa aaggtcgata
    29701 tggtcgatgt tttccgtaat tcagaagcgg cgtggggcgt ggcacaggaa gctattgcca
    29761 ttggcgcgaa aaccctctgg atgcaactgg gggtgattaa tgagcaagcg gcagtactgg
    29821 cacgggatgc cgggttaaat gtggtgatgg atcgctgccc ggctattgag atccctcgcc
    29881 tgggcctggc caaataaaaa atccccggaa ggcaaaaacc ttccggggat tcgttcaggg
    29941 attagttacg cagacgcggg gcctggagtt gtttgcggat ggtctgcgcc agctcatcca
    30001 tggatggctg ttcaggatgt tcatcctgca attcgctact tagctgggct tcagccaggt
    30061 aggtatgaac cggtaggccg ttatcgtcct ccatcaccac gtgataccac ggagcggcgc
    30121 gaagctcgtc attcaccgcc agctcatcag gcgacggttc agagagcgaa taaaccgggt
    30181 cgatatccac gaccactccg agataaccta acagggaatg gcggacctgc tggccgatac
    30241 cgaatttgct ggcaatcata gtcacctccc gggaaatctt tacctttgat ataagggtaa
    30301 aattccacat ttcaagttac atgacgcgac aggcaaaccc tttcagatat agcccttccg
    30361 ggtaggtagc gatcaccgga tgatcggctg cctgacggaa ctgctctata aattgtacat
    30421 cacggccggc atcaattgcg gcatccgcga tgattttctg aaataaatcg ctggtcatca
    30481 gaccggaaca ggagaaagtc aggagaatac cgccttcatt cagcaactga atcgccagca
    30541 tgttgatatc tttatagcca cgacacgcgc ccatcaactg gcttttattc tcaacaaact
    30601 tcggcgggtc catcacgata acgtcaaatt tttcaccgcg atcgcgataa gtacgcagca
    30661 atttaaagac atcatcacgg acaaactcag ccttgctcag atccagtttg ttcagctcaa
    30721 cgttctgccg tgcaatatcc agcgcttcct gggaggtatc aacgctgaca acctggctgc
    30781 aaccgcccat cagtgccgat acggcgaaac caccggtata ggagaaacag ttcagcacac
    30841 gtttattttc aacgtagcgg cgggtagcca ggcggctatc acgctggtcc aggtagtagc
    30901 ccgttttgtg tccgtgctga atatccacca gcagtttcat gccgtgttct tcaatcggca
    30961 gcagggcagg tggcaactcg ccggtgacga ggccctgggt cagctccatc ccttcttttt
    31021 tacgtaccgc aacatcgctg cgatcgtaaa tcgcacattc cgggtacagc gtttgcaggg
    31081 cactaattaa tgccgcgcgc tgatattctg cgccagcact cagcagttgc agcaccagaa
    31141 aattaccgaa acgatcgata gtaatacccg gcaggccatc agattctccg gcgattaaac
    31201 gatagctgtc gaggccatct ttttgcgcca gccagtcacg ccatttttgt gcttgttgca
    31261 aacggcggga aaaaaaagca atgtcgatag actcagacgg gtcaaacgtc cagacgcgcg
    31321 cccggatttg cgaagctggt gaataagcgc cgcgtgctaa ccattttccc tgatgatcaa
    31381 caatatcgat ggtttcaccg aggctggctt taccttccat gcgggcaacg gccccggaaa
    31441 agacccacgg atggcgacga agtaatgatt tttcgcgccc tttggctaac actaaacgta
    31501 cactcataat ttacttttct gtcgatgcaa agaaatgggc gtcattgtcc ggagttttcc
    31561 atggatttgc aacgcgtcca ttaaggataa ggagagcagc aatgtcgaaa gtctgcataa
    31621 ttgcctgggt ttacgggcga gttcagggcg taggatttcg ctacaccaca cagtatgaag
    31681 cgaaaagact gggtttaacc gggtacgcca aaaatcttga cgatggcagc gtggaagtgg
    31741 ttgcctgcgg tgaagaaggg caggtggaaa aattaatgca gtggttaaag agtggcggcc
    31801 cgcgttctgc gcgtgtggaa cgggtgctta gcgagccgca tcatccctcg ggggaattag
    31861 ccgattttcg tatccgttaa atacatttta ccggttttgg caggccagca attttggtgg
    31921 cttgcttagc cggacctttc ggaaacagtc gatataaata gcggctgtta cctttttctt
    31981 cgccaaattt attcgccatc gcttttacca gcatacgaat cgccggagaa gtattgaatt
    32041 ccagatagaa atcacgcaca aaacgcacca cttcccagtg ttctggcgac agcgaaatcc
    32101 cttcgttctc tgcaatcacc acagccagcg gctcactcca ctggctgctt tctttgagat
    32161 agccttcggt atccgtttct atctctttac cttcgaagat cagcataatt attactacct
    32221 taatcagact ggcggcagtg taacaaaaaa caaagccccg cataaagcga ggctatgaaa
    32281 gtgttagcgg gtgagattaa tcgcggctag cgaagcccag aatgctcagc aggctgacga
    32341 agatgttgta cagcgaaaca tacaggctaa ccgtggcacg aatatagttc gtctcaccgc
    32401 catgaatgat gttgctggtt tcaaacaaga tagcgccaga ggagatcaga atgaagaccg
    32461 cgctgatcgc cagatgcaga gcaggcagtt gcaggaagat attagcaacc ataccaatca
    32521 gcaccaccac aatacccgcc atcagcatac cgccgaggaa cgacatatct ttgcgggtgg
    32581 tcagcacata tgcagagcag cagaagaaca ctaacgccgt tccgcccagt gccatagcga
    32641 ttacgtcacc cattccggca gacagatagg tgttcagaat aggtccgagg atataaccca
    32701 gaaaaccggt aaaggcgaat gcggagataa tcccggtcgg cttattcgcc gttttatagg
    32761 tcaggaacat caaaccatac atacccacca gcgtcagaat cagacccgga gatggcagca
    32821 tcagcaccgt actggcagtt gcggtaatcg ccgaaaaggc cagcgtcagg ctcagcagaa
    32881 aataggtatt acgcagcacc ttatgggtgc taagcagtga tgtacggtca tgtgaagaac
    32941 taacaatacg atccattgag tcactctcta tgacagatgt aattaattaa gcagcataat
    33001 gataatgcgt aagggcaccc agaagtttta cccatcttta cgcatttgat ctggaacagg
    33061 tttaacagcg gattatcagg tcattaagca aatataacgc cctgagaatt tcgacaggca
    33121 aaagaaaaag gggttagcat ttagctaacc ccttatctta tttggcggaa gcgcagagat
    33181 tcgaactctg gaaccctttc gggtcgccgg ttttcaagac cgaagaaaaa caaataaaaa
    33241 tcagtgtaat acgtgaagct gctgaaaatt taatcaaatt taacaccaag aaaaatcaag
    33301 gtgttaggaa gttgctcata gctagtatac caagtatttt tgccagaaaa ttttctgtat
    33361 tccctacttg ctaaccacga gcggcagatc cagtgttggt gtgatttttg ttttacgatc
    33421 gtaaatcaac acctgatttt ctgttttatg cccgctgaaa atttgtttgt cgcgactgct
    33481 gccttcgtaa tctgagatcc ccttggcttt tatatcgtga aaattgcacc cgaacggaac
    33541 gccaactttt tgctcggctg agcgtttagc ctgattccac cagttgttca gcgttttagc
    33601 tatgactttc ccgcctttgg ttgtattgat cacatattcg catgtacagg aagatacatt
    33661 tcgggctaac tggatcgctg tacgtaatcg cggtgaccat tccttgattt gtttggtgcc
    33721 ggttttgttt tgctcaatgt agatcccttt atccataata tcctgccatt tcaactcaag
    33781 cacatcaccg agccttgccg cacagagata ggaaatctcc attgcaatgc gtaactgtgg
    33841 aattgcttcc gcatatatcg ctgcatactg ttcatcggtg atgtaaacag tgcgggcttt
    33901 aagagagaat tttctgactc ctttgcatgg gttattcttc acgtatccac gctcatatcc
    33961 ccacccgtat actcgactca ggcttgccag ttcatgattt gcttgggtct tgctctcaag
    34021 ccctcgttta tccatgaaaa ttcttacctg ctcagttttg acattatcag caagcacttt
    34081 tccgaatacc atcagcaatg ccttctgatg ttgtcgataa tctttttggg ttcggggggc
    34141 cagttctgta aatgcagggg agtccataaa catgtgccat aatttagcta cggtcattat
    34201 gttgtggagt tttgcttttt ccagttcata attttgccag actttagcta cgctggtttt
    34261 ccgcactctt cctagcccta tagttcttgt acttccttct ggtttccaca cgtaactgta
    34321 accattcgat ctaacccgcg gtggcagtac attatctttt ttattttttc ttggtcttcc
    34381 cattgttcag cgcctcaaaa tcgggttcag cagaaaccag ttcagatgtt tttggcatcg
    34441 ttgtcagtcc gtgtggaata tccctgcgta gaactattgg ttcgttttta ggaccgatta
    34501 caaatgggat gccgtgcagt cttaactggt gttgctgttt tgtgtatcgc tcgtatttcg
    34561 tgatctcttt aatctctgct ggagatagag ttaattcgta catgtggtca cgtgccttta
    34621 cagcatgacc gccgccacta tagctggtgg gcggcgatat gggttgaaca ttaaaaatca
    34681 gcctgactcg ggatcagttt ttgccagata gctgaaacgt attttgcctg ataacgggcg
    34741 tcatcaagtg cattatggcg ctcaccttcg aatggaatag ccgttctggc atcgaagtct
    34801 atggctttcc ccagctcaac gattgtgcgt acatcgcgat cgttgtagta acgccacggg
    34861 caggggatcc cctgccgttc gtatgaacgg cgcaaaatcg tgttgtcgaa gttggctcca
    34921 tttccccaga cctgaacaaa aaattcaccg gagttttcgt cgataaattc ccgcagttgt
    34981 aacagtgcat catctaacgg gatttcatcg gtcataatgg cagattgcgc ttcgcgtgat
    35041 tgcttaagcc accatttaat ggtgtcccga tcaatgactc cgccagcagt ttccagatcg
    35101 atagtcttac taaattccgg tcccatatct ccggtttgcg gatcgaaaaa tattgcacct
    35161 attgagatga tcggggcatc aggatttttt cccatggttt caaggtcgat cattagatgg
    35221 tcacacgtcc tgctggtgga tgtgatttcg tgatgaccgt tcaccgcaat taagggatct
    35281 gctgcctcgc cagtttcact atcgctggtg tgatgctgat cgttaccagc attctcattg
    35341 ttcggatgtt cagcgccttc tatttcctcc ggatcatctt cctgaacttc agcctgattt
    35401 ttttcatcga atgtttcctg gtatgttgcg tcgcccatca ccgcgccaca gtcaggacag
    35461 ttgccgccac cgctctgacc gcaggcggtg cagacttttt cctcttcctg ttgctgttgc
    35521 gctactggtt cggattgttt cgtttctggc tcgtcttgta acgcattttg gctgttttgt
    35581 tctgctttct ggtcgttctg ttccgtttct tgctggttct ggttcacaga atcgcaggtt
    35641 tcaatcccct taacccattt cgaatcattc gggtcgctaa tcccttcaac aaactcacca
    35701 cgagaagcgg cgagcaattt gtcggcatct ggtcgactga tgtctgctgc ctccataatt
    35761 ttatttactt cttcagcggt aacttttacc tgtgacttgt catccagcgg ctgcgggtcc
    35821 tgatgatgtt caactgtatc cgctgccatt gtttcagccg ttgcctgttc atctgccatt
    35881 gcgccagatg gttgcgggtt ttcttcatca tttttttctt cttctgtttt gcactcagta
    35941 gccagtccgc cgttaatttc ttccaggata tctttttccg gtgtatgccg ggcagccatg
    36001 agcgtttcag ctgtgggatt ctcgtgatca gtttctgtca ggttggcatt gatatacccc
    36061 tgaaggcgtc ccgggtagtg ataaaactca gggtgggcgc tccggataag tgcaaaaata
    36121 gctgcgcggg aatagtccag gataccaggg gttgcgcgaa gtgctgcgga ccattccctg
    36181 aacggacttt cttttttcag gacgatttct tttgcgcgac gataaacgct cacgggtatt
    36241 tcataaatat taaaatccat cggaagcgtg gctgctgcag cctccacatc cagagtatcg
    36301 agggtgtgta ctaagtcagg attacgatcg gttttatttc cgccaccggc atttgcaccg
    36361 gaagccgtgc gggtgatgcg agaaacacga tttcctttca tccactcttt tgtcagcaga
    36421 cctcgatcgg tatagtcagc gtccaggtat gcttcgaaaa aagtagtcat taaccccaga
    36481 ctggaattaa cgggattagg gaaaactttg tctgtgtcgc gtaccagttt atgaaggtcg
    36541 cgaatctcca gcgggtcgag cagctttgtg ttgtgggaaa tagccagggc agtaacagcc
    36601 ggcagctctt cagctcgtgc gatatgtaat gcctgtagtt cttcccgtga aacgtgcgtt
    36661 accggttttt cgctgccatg ttgcgcaagc cagcgaatgg gcagttcctg accggaaatc
    36721 ggcaggagca tattatcctc aatctcagcc atgtcttcgc cattgacgtt ggtattgtca
    36781 gtgctggctg gtttgtcctg aactgagggg gaaggcgcta taaatatcat tgtgatgcca
    36841 tcttccccgc ctttttcata gcggttgcag aattcggtat caaacacgcc ttccggtgga
    36901 aggtcattca caacggggaa atggacgcgg acaggttttt tgaaatcatc agcgtcgtag
    36961 cctgcctc
//
LOCUS       DAZFFI010000039        35555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFFI010000039 DAZFFI010000000
VERSION     DAZFFI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.039"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHJ12_002251"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHJ12_002251"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4990030.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHJ12_002252"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHJ12_002252"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4990031.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /gene="ysaB"
                     /locus_tag="ACHJ12_002253"
     CDS             complement(3139..3438)
                     /gene="ysaB"
                     /locus_tag="ACHJ12_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4990032.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="ACHJ12_002254"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="ACHJ12_002254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4990033.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACHJ12_002255"
     CDS             complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACHJ12_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4990034.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGYYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACHJ12_002256"
     CDS             complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACHJ12_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4990035.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5643..7097)
                     /gene="xylB"
                     /locus_tag="ACHJ12_002257"
     CDS             complement(5643..7097)
                     /gene="xylB"
                     /locus_tag="ACHJ12_002257"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4990036.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7169..8491)
                     /gene="xylA"
                     /locus_tag="ACHJ12_002258"
     CDS             complement(7169..8491)
                     /gene="xylA"
                     /locus_tag="ACHJ12_002258"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4990037.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8857..9849
                     /gene="xylF"
                     /locus_tag="ACHJ12_002259"
     CDS             8857..9849
                     /gene="xylF"
                     /locus_tag="ACHJ12_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4990038.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9927..11468
                     /gene="xylG"
                     /locus_tag="ACHJ12_002260"
     CDS             9927..11468
                     /gene="xylG"
                     /locus_tag="ACHJ12_002260"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4990039.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11446..12627
                     /gene="xylH"
                     /locus_tag="ACHJ12_002261"
     CDS             11446..12627
                     /gene="xylH"
                     /locus_tag="ACHJ12_002261"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4990040.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12705..13883
                     /gene="xylR"
                     /locus_tag="ACHJ12_002262"
     CDS             12705..13883
                     /gene="xylR"
                     /locus_tag="ACHJ12_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4990041.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13991..14815)
                     /gene="bax"
                     /locus_tag="ACHJ12_002263"
     CDS             complement(13991..14815)
                     /gene="bax"
                     /locus_tag="ACHJ12_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4990042.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15135..17165
                     /gene="malS"
                     /locus_tag="ACHJ12_002264"
     CDS             15135..17165
                     /gene="malS"
                     /locus_tag="ACHJ12_002264"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4990043.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17343..18596
                     /gene="avtA"
                     /locus_tag="ACHJ12_002265"
     CDS             17343..18596
                     /gene="avtA"
                     /locus_tag="ACHJ12_002265"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4990044.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEEGVKILAEEIERAWAESH"
     gene            complement(18748..19221)
                     /gene="ysaA"
                     /locus_tag="ACHJ12_002266"
     CDS             complement(18748..19221)
                     /gene="ysaA"
                     /locus_tag="ACHJ12_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4990045.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19323..20171)
                     /gene="yiaJ"
                     /locus_tag="ACHJ12_002267"
     CDS             complement(19323..20171)
                     /gene="yiaJ"
                     /locus_tag="ACHJ12_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO4990046.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20372..21370
                     /gene="yiaK"
                     /locus_tag="ACHJ12_002268"
     CDS             20372..21370
                     /gene="yiaK"
                     /locus_tag="ACHJ12_002268"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO4990047.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21382..21849
                     /gene="yiaL"
                     /locus_tag="ACHJ12_002269"
     CDS             21382..21849
                     /gene="yiaL"
                     /locus_tag="ACHJ12_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO4990048.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21967..22440
                     /gene="yiaM"
                     /locus_tag="ACHJ12_002270"
     CDS             21967..22440
                     /gene="yiaM"
                     /locus_tag="ACHJ12_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO4990049.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALGTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22443..23720
                     /gene="yiaN"
                     /locus_tag="ACHJ12_002271"
     CDS             22443..23720
                     /gene="yiaN"
                     /locus_tag="ACHJ12_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO4990050.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23733..24719
                     /gene="yiaO"
                     /locus_tag="ACHJ12_002272"
     CDS             23733..24719
                     /gene="yiaO"
                     /locus_tag="ACHJ12_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO4990051.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24723..26219
                     /gene="lyxK"
                     /locus_tag="ACHJ12_002273"
     CDS             24723..26219
                     /gene="lyxK"
                     /locus_tag="ACHJ12_002273"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO4990052.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIGEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDESTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26216..26878
                     /gene="ulaD"
                     /locus_tag="ACHJ12_002274"
     CDS             26216..26878
                     /gene="ulaD"
                     /locus_tag="ACHJ12_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4990053.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            26871..27731
                     /gene="sgbU"
                     /locus_tag="ACHJ12_002275"
     CDS             26871..27731
                     /gene="sgbU"
                     /locus_tag="ACHJ12_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO4990054.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27725..28420
                     /gene="araD"
                     /locus_tag="ACHJ12_002276"
     CDS             27725..28420
                     /gene="araD"
                     /locus_tag="ACHJ12_002276"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4990055.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28452..28553)
                     /locus_tag="ACHJ12_002277"
                     /pseudo
     CDS             complement(28452..28553)
                     /locus_tag="ACHJ12_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28557..28820
                     /locus_tag="ACHJ12_002278"
                     /pseudo
     CDS             28557..28820
                     /locus_tag="ACHJ12_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28767..29507)
                     /gene="yiaT"
                     /locus_tag="ACHJ12_002279"
     CDS             complement(28767..29507)
                     /gene="yiaT"
                     /locus_tag="ACHJ12_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4990056.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSIFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            29631..30605
                     /gene="yiaU"
                     /locus_tag="ACHJ12_002280"
     CDS             29631..30605
                     /gene="yiaU"
                     /locus_tag="ACHJ12_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4990057.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(30602..31738)
                     /gene="yiaV"
                     /locus_tag="ACHJ12_002281"
     CDS             complement(30602..31738)
                     /gene="yiaV"
                     /locus_tag="ACHJ12_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4990058.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEIAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(31744..32067)
                     /gene="yiaW"
                     /locus_tag="ACHJ12_002282"
     CDS             complement(31744..32067)
                     /gene="yiaW"
                     /locus_tag="ACHJ12_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4990059.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            32250..32393
                     /locus_tag="ACHJ12_002283"
     CDS             32250..32393
                     /locus_tag="ACHJ12_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990060.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(32612..34150)
                     /gene="aldB"
                     /locus_tag="ACHJ12_002284"
     CDS             complement(32612..34150)
                     /gene="aldB"
                     /locus_tag="ACHJ12_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4990061.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(34315..35466)
                     /gene="yiaY"
                     /locus_tag="ACHJ12_002285"
     CDS             complement(34315..35466)
                     /gene="yiaY"
                     /locus_tag="ACHJ12_002285"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4990062.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         8810 a         9150 c         8854 g         8741 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccagaatg
      361 ttagatacac gtttgttcgc cgccgccagt gcagcagctg catccagggt acggaaatgc
      421 gataccgctt tcatacgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt ataaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aactacgttg gcattagtca gcttatcgcc atacagacgc
      601 accgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg
      721 ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct
      781 accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc
      841 accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtgt tgaagaagaa ctcggcatcc
     1141 gccagacgcg gacgaacgac tttctcgtta ccggagataa tctgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat aaagttcggc agcagtttgc cgtcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accagcgctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct
     1741 tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc
     3361 tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacct gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tatccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgcct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa taaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata
     4861 taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac
     4921 gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc
     4981 atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag agctatccat
     5041 gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt
     5101 tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga
     5161 ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt
     5221 gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata
     5281 tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca
     5341 aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag
     5401 atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt
     5461 tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt aaccaacgaa
     5521 agacaaaact gaaaaatgcc attaacaaat gattttcaga ataaattcat actaaatatt
     5581 aattaattag tgagatatat agatgtgaat tatcccccac ccggtcaggc aggggataac
     5641 gtttacgcca ttaatggcag aagttgctga tagaggcgac ggaacgtttc acgtcgtggc
     5701 tgataagcgg cataacgctg cgcatctggt agatgcgact gttctaacgg tagctgcggc
     5761 aacaactcaa tgagcgattt ctctggattc gccgcaatct gcgccagcct tgctgcgccc
     5821 agtgctggcc ccacatcccc ccccgtacgg taatcgagct gctgaccgct gatatccgcc
     5881 agcatctgac gccagtactc actacgcgcc ccgccaccaa tcaacgtaac actttgcggt
     5941 ttaatgccgc aggcatgcac gacatccatg ccatctgcca gcgcataacc cacgccttcc
     6001 agcactgctc gcgccagttc attggggcca tgttgatgag tcaaaccaaa gaaaaccccc
     6061 ttcgcctggg gattattgtg tggtgtacgc tcgccggaaa gataaggcag aaaccaaact
     6121 ggctcggcac tttcatcagc ctgttgagct gcagcgatta aagctgggac attgcacagg
     6181 ccggttaatt tcgcgaccca atccagacac gatgctgcac tcagcattac agacattaaa
     6241 tgccaacgct gcggtagcgc atggcaaaag ctgtgtacag cgctttctgg cttgcttaag
     6301 aacccttcgc tgacagcaaa atagaccccc gacgtcccca gcgataacat tgcctgatta
     6361 gcatcaacca ttcccacacc aactgcacca gctgcattgt cgccaccgcc tgcgacaact
     6421 ggcaccgtcg ccatacccca cgctttcgca acttcaggta acaaagcacc agtaatttcg
     6481 ctgccttcat ataatgcggg catctggtca cgagataagt cgcaagcctg cagcatgacg
     6541 tcactccagt cacgctttgc gacatccagc cacatggtgc cagctgcgtc agacatatcg
     6601 ctggcaaact cccccgtcat acgcagacgc aagtaatctt tcggtaataa tactttgtcg
     6661 atttgacgga atatctccgg ctcatgccgc tgaacccata gcaatttagg cgcagtaaat
     6721 ccgggcatca tcaggttgcc ggtaatcact cgtgattgcg gaactctcgc ttccagcaaa
     6781 gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg cagggcgcaa tacccgttgt
     6841 tgagcatcca gtaaggttgc tccatgcatc tggccggcaa tacccaatgc tttaacgtcc
     6901 tgcagagaat gctgatcgcc cagagctttc attgcgcgat cagttgcctg ccaccactgt
     6961 tccgggtctt gttccgacca gagtggatgc gggcgcgaaa cggtcagctt ttccgtttgc
     7021 gaagcaacca cctcaccctg ctcgttgagc aaaataactt ttacgcccga ggtgccaaga
     7081 tctatcccga tatacatatc gatcgttcct taaaaaaatg cccggtatcg ctaccgataa
     7141 ccgggccaac ggactgcaca gttagccgtt atttgtcgaa cagataatga tttaccagat
     7201 tttccagttg ctcctggcga ccactctgat gcaccggaga caaattatgt tcctgagcat
     7261 atttggctaa atctgccagt gacatttggc ctttcaggat ttgctggccc aattcgctat
     7321 tccagccgga ataacgctgc gcgatgcgtt tatccagctc gccatcttca atcatgcgcg
     7381 ctgcaatttt cagcgccagt gccatcgtat ccatcgcgcc gatatgaccg taaaacagat
     7441 catatttatc agtactttga cgacgtactt tggcatcgaa gttcaaacca ccggtggtga
     7501 aaccgcctgc tttgagaatt tcatacatca ccagcgcatt ctcttccaca ctgttcggga
     7561 actggtcggt gtcccagccc agttgcgcat cgccacggtt ggcgtcgaca gaaccgaaca
     7621 ggccaagcgc aatggcggtg gctatttcat gatggaaaga gtgacctgcc agcgtcgcgt
     7681 ggttagcttc aatgttcagt ttaatctctt tttccagacc aaactgtttc aggaagccat
     7741 agaccgtcgc ggcatcgtaa tcatattgat gtttagtcgg ttcttgcggt ttcggttcga
     7801 taagcaacgt gccctggaag ccgattttat gtttatgctc aaccaccatc tgcataaagc
     7861 ggcccagttg ttcacgctcc tgacgcaagt cggtatttaa cagcgtttcg taaccttcac
     7921 gaccacccca caggacatag ttttcaccgc ccaatttatg ggttgcttcc atcgctgtaa
     7981 caacttgcgt tgccgcccag ctgaagactt caggatctgg gttcgtcgcc gcacccgcgc
     8041 cgtagcgagg gtttgtaaag cagttggcgg ttccccacag cagcttcacg ccgctctctt
     8101 cttgcttgcc tgccaggaca tcaaccattt gcgcaaaatt attgatgtac tcttttaacg
     8161 acgcgccctc aggggaaaca tccacatcgt ggaagcaata aaatggcaca tgtaacttgt
     8221 ggaaaaactc aaatgcgaca tctgctttac gcttcgccaa cgccagtgcc tcaccaggct
     8281 gctgccacgg acgattaaac gcccccacac caaacatgtc cgccccgttc cagcagaagg
     8341 tgtgccagta gcaggcggca aaacgcaagt gctcttccat acgcttaccc aacaccagtt
     8401 cgtcgggatt gtagtgacgg aatgctaacg ggtttgagga ttttgagcct tcataacgaa
     8461 cgcgatcgag ctggtcaaaa taggcttgca tattgaactc cataatcagg taatgccgcg
     8521 ggtgatggat gatgtcgtaa tattgggcac tccctttcag ttgctcaatt atgttatttc
     8581 acactgctat tgagataatt cacaagtgtg cgctcgctcg caaaataaaa tgggatgatg
     8641 aaactgggta attcctcgaa gataaaaatg caataagtac aattgcgcaa caaaagtaag
     8701 atctcggtca taaatcaaga aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa
     8761 ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga ttgttactta
     8821 ttaaagctgt cctctaacta cagaaggccc tacaccatga aaataaagaa cattctactc
     8881 accctttgca cctcacttct gcttaccaac gttgcggcac acgccaaaga agtcaaaata
     8941 ggtatggcga ttgatgatct ccgtcttgaa cgctggcaaa aagatcgaga tatttttgtg
     9001 aaaaaggcag aatctctcgg cgcgaaagta tttgtacagt ctgcaaatgg caatgaagaa
     9061 acacaaatgt cgcagattga aaacatgatt aaccggggtg tcgatgttct tgtcattatt
     9121 ccgtataacg gccaggtatt aagtaacgtt gtaaaagaag ccaaacaaga aggtattaaa
     9181 gtattagctt acgaccgtat gattaacgat gcggatatcg atttttatat ttctttcgat
     9241 aacgaaaaag tcggtgaact gcaggcaaaa gccctggtcg atattgttcc gcaaggtaat
     9301 tacttcctga tgggcggctc gccggtagat aacaacgcca agctgttccg cgccggacaa
     9361 atgaaagtgt taaaacctta cgttgattcc ggaaaaatta aagtcgttgg tgaccaatgg
     9421 gttgatggct ggttaccgga aaacgcattg aaaattatgg aaaacgcgct aaccgccaat
     9481 aataacaaaa ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg ggcaattcag
     9541 gcattaagcg cgcaaggttt atcagggaaa gtagcaattt ccggccagga tgcggatctc
     9601 gcaggtatta aacgtattgc tgccggtacg caaactatga cggtgtataa acctattacg
     9661 ttgttggcaa atactgccgc agaaattgcc gttgagttgg gcaatggtca ggaaccaaaa
     9721 gcagatacca cactgaataa tggcctgaaa gatgtgccct cccgcctcct gacaccgatc
     9781 gatgtgaata aaaacaacat caaagacacg gtagttaaag acggattcca caaagagagc
     9841 gagctgtaag cgttacgccc cggcgcggag cgggggcgtg atttctctcc atgccgcgtg
     9901 aatgaattgg cttaggtgga gtcgttatgc cttatctact tgaaatgaag aacattacca
     9961 aaaccttcgg cagtgtgaag gcgattgata acgtctgctt gcggttgaat gctggcgaaa
    10021 tcgtctcact ttgtggggaa aatgggtctg gtaaatcaac gctgatgaaa gtgctgtgtg
    10081 gtatttatcc ccatggctcc tacgaaggcg aaattatttt tgcgggagaa gagattcagg
    10141 cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat cattcatcag gaattggccc
    10201 tggtgaaaga attgaccgtg ctggaaaata tcttcctggg taacgaaata acccacaatg
    10261 gcattatgga ttatgacctg atgacgctac gctgtcagaa gctgctcgca caggtcagtt
    10321 tatccatttc acctgatacc cgcgttggcg atttagggct tgggcaacaa caactggttg
    10381 aaattgccaa ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa ccgacagcct
    10441 cattaactga gcaggaaacg tcggttttac tggatattat tcgcgatcta caacagcacg
    10501 gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt caaagcgatt tccgatacga
    10561 tttgcgttat tcgcgacgga cagcacattg gtacgcgtga tgctgccgga atgagtgaag
    10621 acgatattat caccatgatg gtcgggcgag agttaaccgc gctttaccct aatgaaccac
    10681 ataccaccgg agatgaaata ttacgtattg aacatctgac ggcatggcat ccggtcaatc
    10741 gtcatattaa acgagttaat gatgtctcgt tttccctgaa acgtggcgaa atactgggta
    10801 ttgccggact cgttggtgcc ggacgtaccg agaccattca gtgcctgttt ggcgtgtggc
    10861 ccggacaatg ggaaggaaaa atttatattg atggcaaaca ggtagatatt cgtaactgtc
    10921 agcaagccat cgcccagggg attgcgatgg tacccgaaga cagaaagcgc gacggcatcg
    10981 ttccggtaat ggcggttggt aaaaatatta ccctcgccgc actcaataaa tttaccggtg
    11041 gcattagcca gcttgatgac gcggaagagc aaaaatgtat tctggaatca attcagcaac
    11101 tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg tttgagcggc ggcaatcagc
    11161 aaaaagcgat cctcgctcgc tgtctgttac ttaacccgcg catcctcatt cttgatgaac
    11221 ctaccagggg tatcgatatt ggcgcgaaat acgagatcta caaattaatt aaccaactcg
    11281 tccagcaggg tattgccgtt attgtcatct cttccgaatt acctgaagtg ctcggcctta
    11341 gcgatcgggt actggtgatg catgaaggga aactaaaagc caacctgata aatcataacc
    11401 tgactcagga gcaggtgatg gaagccgcat tgaggagcga acatcatgtc gaaaagcaat
    11461 ccgtctgaag tgaaattggc cgtaccgaca tccggtggct tttccgggct gaaatcactg
    11521 aatttgcagg tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt ctttacctgg
    11581 accaccgatg gtgcctactt aagcgcccgt aacgtctcca acctgttacg ccagaccgcg
    11641 attaccggca tcctcgcggt aggaatggtg ttcgtcataa tttctgctga aatcgacctt
    11701 tccgtcggct caatgatggg gctattaggt ggcgtcgcgg cgatttgtga cgtctggtta
    11761 ggctggcctt tgccacttac catcattgtg acgttggttc tgggactgct tctcggtgcc
    11821 tggaacggat ggtgggtcgc gtaccgcaaa gtcccttcat ttattgtcac cctcgcgggc
    11881 atgttggcat ttcgcggcat actcattggc atcaccaacg gcacgactgt ttcccccacc
    11941 agcgccgcga tgtcacaaat tgggcaaagc tatctacctg ccagcactgg cttcatcatt
    12001 ggcgcgcttg gcttaatggc ttttgttggt tggcaatggc gcggaagaat gcgccgtcag
    12061 gctttgggtt tgcagtctcc ggcctctacc gcagtagtcg gtcgccaggc tttaaccgct
    12121 atcatcgtat taggcgcaat ctggctgttg aatgattacc gtggcgttcc cactcctgtt
    12181 ctgctgctga cgttgctgtt actcggcgga atgtttatgg caacgcggac ggcatttgga
    12241 cgacgcattt atgccatcgg cggcaatctg gaagcagcac gtctctccgg gattaacgtt
    12301 gaacgcacca aacttgccgt gttcgcgatt aacggattaa tggtagccat cgccggatta
    12361 atccttagtt ctcgacttgg cgctggttca ccttctgcgg gaaatatcgc cgaactggac
    12421 gcaattgcag catgcgtgat tggcggcacc agcctggctg gcggagtggg aagcgttgcc
    12481 ggagcagtaa tgggggcatt tatcatggct tcactggata acggcatgag tatgatggat
    12541 gtaccgacct tctggcagta tatcgttaaa ggtgcgattc tgttgctggc agtatggatg
    12601 gactccgcaa ccaaacgccg ttcttgattt tgataaaaat tttctcaaag ccggttacgt
    12661 attaccggtt ttgagttttt gcatgattca gcaggaaaag aaccatgttt actaaacgtc
    12721 accgcatcac attactgttc aatgccaata aagcctatga ccggcaggta gtagaaggcg
    12781 taggggaata tttacaggcg tcacaatcgg aatgggatat tttcattgaa gaagatttcc
    12841 gcgcccgtat tgataaaatc aaggactggt taggagatgg cgtcattgcc gacttcgacg
    12901 acaaacagat cgagcaagcg ctggctgatg tcgacgtccc cattgttggg gttggcggct
    12961 cgtatcacct tgcagaaagt tacccacccg ttcattacat tgccaccgat aactatgcgc
    13021 tggttgaaag cgcatttttg catttaaaag agaaaggcgt taaccgcttt gctttttatg
    13081 gtcttccgga atcaagcggc aaacgttggg ccactgagcg cgaatatgca tttcgtcagc
    13141 ttgtcgctga agaaaagtat cgcggagtgg tttatcaggg gttagaaacc gcgccagaga
    13201 actggcaaca cgcgcaaaat cggctggcag actggctaca aacgctgcca ccgcaaaccg
    13261 ggattattgc cgttactgac gcccgagcgc ggcatattct gcaagtatgt gaacatctac
    13321 atattcccgt accggaaaaa ttatgcgtga ttggcatcga taacgaagaa ctgacccgct
    13381 atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg ggctatcagg
    13441 cggcaaaact gttgcatcga ttattagata aagaagaaat gccgctacag cgaattttgg
    13501 tcccaccagt tcgcgtcatt gaacggcgct caacagatta tcgctcgctg accgatcccg
    13561 ccgttattca ggccatgcat tacattcgta atcacgcctg taaagggatt aaagtggatc
    13621 aggtactgga tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt aaagaagagg
    13681 tgggtgaaac catccatgcc atgattcatg ccgagaagct ggagaaagcg cgcagtctgc
    13741 tgatttcaac caccttgtcg atcaatgaga tatcgcaaat gtgcggttat ccatcgctgc
    13801 aatatttcta ctctgttttt aaaaaagcat atgacacaac gccaaaagag tatcgcgatg
    13861 taaatagcga ggtcatgttg tagcgatgat gagaattgtc ggcgtcacat cagggaactc
    13921 agcacagcca cctgatgcga gcaaaagcct tatccgaccg acaaatcaga taaggctcaa
    13981 ggaaatgcaa ttacatatgc gccgcgatta accgttggtt atcctggtac attgcgaaca
    14041 ggtagttatt ataactcttc cctttggtcg agtagccctt cagcttgtga atcatcgctg
    14101 tggcagtcac ttcctgatcc gctttacgca gctgcgcacg cgatttacgg aacgaagagt
    14161 aagccgggtg cgtattcagg ttagtgacat aggcgctcac cgattctttg acagaactaa
    14221 actgtgagta ccctttcact ttacccggcg cattggtaca acgtcctttc atgcatttca
    14281 tgccgaacag attgttattg ttgcgcgcca gcttcgacgt tccccaaccg ctttctgctg
    14341 cagccatcgt cgccaccata ctggtaggga taatgtctac gcgttcaagc aaggtattcc
    14401 acgggatttt tcgcgtatta ccagaccact tcaccttgta gcgtttggcg atgtctttca
    14461 gacgcgcacg ctcagcaggt gaccattggc cctgatactg ttttgaaatc agccagttac
    14521 gttctgcagt aatggctgca ttttggctgg taatgtaagg cattacggtc cggagaaacg
    14581 cctttttcct tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg
    14641 cactattgcg agaatactct tgtttactgc ttacctgttt attactagct ttagttaagt
    14701 gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca
    14761 gagtgagtaa cataagaatc atcgccccat atcgtcgtat gggagtcaaa atcatcaggt
    14821 ctcctggtcg gatttaatca ttccaacacc ttatattttt cacaaatttg agagttgagt
    14881 ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag gaatagtcca aatctgaaac
    14941 tatgtcacgt gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg ggtttatcgc
    15001 aaaattcagc cgttttttgc gcgagatcgc tcacccttgc ttctcatcct gtggacttac
    15061 cgctcaggga tgagttttgt ctggcttatc gctggcaaac tgtctgaaat cgcagcaata
    15121 aggactcatc caccatgaaa ctcgccgcct gttttctgac actccttcct ggcttcgccg
    15181 ttgccgccag ctggacttct ccggggttcc ctgcctttag cgaacaggga acgggaacat
    15241 ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact cacgctaaat tttgaccaac
    15301 agtgctggca gcctgcagat gcgataaaac tcaatcagat gctttccctg caaccttgta
    15361 gcaacacgcc gcctcaatgg cgattgttca gagacggcaa atatacgctg caaatagaca
    15421 cccgctccgg tacgccaaca ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa
    15481 acctggtccg tgaatgcccg aaatgggatg gattaccgct cacgctggat gtcagcgcca
    15541 ctttcccgga aggagccgcc gtacgggatt attacagcca gcaaattgcg atagtgaaga
    15601 acggtcaaat aacgttacaa cccgctgcta ccagcaacgg tttactcctg ctggaacggg
    15661 cagaaactga cacatccgcc cctttcgact ggcataacgc cacggtttac tttgtgctga
    15721 cagatcgttt cgaaaacggc gatcccagta atgaccagag ttacggacgt cataaagacg
    15781 gtatggcgga aattggcact tttcacggcg gcgatttacg cggcctgacc aacaaactgg
    15841 attacctcca gcagttgggc gttaatgctt tatggataag cgccccattt gagcaaattc
    15901 acggctgggt cggcggcggt acaaaaggcg atttcccgca ttatgcctac cacggttatt
    15961 acacacagga ctggacgaat cttgatgcca atatgggcaa cgaagccgat ctacggacgc
    16021 tggttgatag cgcacatcag cgcggtattc gtattctctt tgatgtcgtg atgaaccaca
    16081 ccggctatgc cacgctggcg gatatgcagg agtatcagtt tggcgcgtta tatctttctg
    16141 gtgacgaggt gaaaaaaacg ctgggtgaac gctggagcga ctggaaacct gccgccgggc
    16201 aaacctggca tagctttaac gattacatta atttcagcga caaaacaggc tgggataaat
    16261 ggtggggaaa aaactggatc agaacggata tcggcgatta cgacaatcct ggattcgacg
    16321 atctcactat gtcgctggcc tttttgccgg atatcaaaac cgaatcaact accgcttctg
    16381 gtctgccggt gttctataaa aacaaaacgg atactcacgc taaagtcatc gaaggcttta
    16441 cacctcgcga ttacttaacc cactggttaa gtcagtgggt ccgcgactat gggattgatg
    16501 gttttcgggt cgataccgcc aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg
    16561 aagccagcgc cgcgcttcgc gaatggaaaa aagctaaccc cgacaaagca ttagatgaca
    16621 aacctttctg gatgaccggt gaagcctggg gccacggcgt gatgcaaagt gactactatc
    16681 gccacggctt cgatgcgatg atcaatttcg attatcagga gcaggcggcg aaagcagtcg
    16741 attgtctggc gcagatggat acgacctggc agcaaatggc ggagaaattg cagggtttca
    16801 acgtgttgag ctacctctcg tcgcatgata cccgtctgtt ccgtgaaggg ggcgacaaag
    16861 cagcagagtt attactatta gcgccaggcg cggtacaaat cttttatggc gatgaatcct
    16921 cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg tacacgttcg gatatgaact
    16981 ggcaggatgt tagcggtaaa tctgccgcca acgtcgcgca ctggcagaaa atcagccagt
    17041 tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacactttcg ctgaagcagg
    17101 gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcatctgg gctgggcaac
    17161 agtgactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc aggataaaat
    17221 gtcagcccta atcagcgttg caggataaag caccgctcac tcttcaacag accgatttgc
    17281 accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac agatatcccg
    17341 ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt acgctgctga
    17401 tggaagatct gaacgacggt ttacgcacgc ctggcgcgat tatgctcggc ggcggtaatc
    17461 cggcgcagat cccggaaatg caggactact tccagacgct actgaccgac atgctggaaa
    17521 gtggcaaagc gactgatgca ctgtgcaatt acgacggtcc acaggggaaa acggagctac
    17581 tcacactgct tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa gcacagaata
    17641 ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaatctg tttgccggac
    17701 gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct ggcaccggaa tacattggct
    17761 acgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat attgaactgc
    17821 tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt ggcgaagaaa
    17881 ccgggatgat ttgcgtctcc cggccgacga atccaacagg caatgtgatt actgacgaag
    17941 agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg attgataacg
    18001 cttatggcgt cccgttcccg ggtatcatct tcagcgaagc gcgcccgctg tggaatccga
    18061 atatcgtact gtgcatgagc ctttccaagc tggggctgcc tggctcccgc tgcggcatta
    18121 tcatcgccaa tgaaaaaatc atcaccgcca tcaccaatat gaacggcatt atcagcctgg
    18181 cacctggcgg tattggtccg gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc
    18241 gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa actatcgcca
    18301 tcattcgccg ctatttaccg gaagatcgct gcctaattca taaaccagaa ggagccattt
    18361 tcctctggct ttggtttaag gatttgccca ttacgaccga gcagctctat cagcgcctga
    18421 aagcacgcgg cgtgctgatg gtgccggggc acaacttctt cccagggctg gacaaaccgt
    18481 ggccgcatac gcatcaatgt atgcgcatga actacgtacc agagccggag aaaattgagg
    18541 agggggtgaa gattctggcg gaagagatag aaagagcctg ggctgaaagt cactaaaaat
    18601 tgcctgatgc gctacgctta ttaggcctac atgatttctg caatatattg aatttgcaag
    18661 aatttgtggg ccggataagg cgtttacgcc gcatctggca taaacaaagc gcactattcc
    18721 ggcgttgaga aacgccggaa aaacgtttca aacaggctgc tgccgtagcc gatgccgctg
    18781 cactttctcg acatccatgc actgcaacgc ctttgtcggg caggcttcaa cacaggccgg
    18841 tcccgtctcc cgatgccagc acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga
    18901 aacgacctcc atcgcaccaa acgggcaagc cagcatacag cttttacagc caatgcaacg
    18961 tgtttgttca acgaaaatat gcccatgttc gcggcttatc gcgtcaacag ggcagacatt
    19021 cgcgcacggt gcatcttcac actgatgaca ggctactgcc gtggtccagc tgtggtcttt
    19081 aatgacacga atacgggaaa taaactcgtc tggtgacaac gcagcgcaat cctgattctc
    19141 ctgatgcgac actgcgcaag ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc
    19201 cgcaataata aaccggttca tgatcactcc tttgcactaa aacagcagag tgccagagag
    19261 cgcgtcccta ctgctttgat ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag
    19321 cgttatgtga tcgcgcccag gtcatccctg acagtaaatc ccagttcatt agaaatagcc
    19381 tgcgcggttt cacgcagtgg tttcaggaga tttttctctc ccacctgttt cagacgtgat
    19441 gtcgaaagcg aaatcgacac ggcgtacggc acccgcccat gaatatcaaa caccggaaca
    19501 gcaatacagg agacgccgag ttcgttttct tctctgtcca tcgccgctcc gctttcacga
    19561 atgtgcgcca gttcgtcgaa catcgcgggc agctcggtaa tggtattgcg ggttaacggc
    19621 tggatctcat gctgatggtt ttcccagtat gacttcacgt agtccgggtg accaaacgcc
    19681 atgtagatct tgcccattgc ggaacagtag agcggcatat gctggccaat ataggcacgg
    19741 gttcgcagca tcccggttgt gggttccagc ttataaatca aaatagcgtg atcgtcttcg
    19801 cggctggaga agttaatggt ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg
    19861 gcgatatgaa tgatattcag cgaagacagc gccttctgcc cgacggcaat aaatttggta
    19921 gtcaggcggt aactccctgc ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc
    19981 tgcaataagc gatggacggt actcttattt aaaccagcca gctccgaaag atgcgccaac
    20041 ggacaaccgt ttggatagtt gctcaaaatc tcaatcagca tcaacccgcg aaacagactc
    20101 tggcttccgg ctggacgctc tttttcctgc gccatctcgt tctctttttt tcccatcact
    20161 tctttcccca ttttgtcgcg tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc
    20221 gacagataat ttataaccac ttgattttta tgtcttttga aattcatcaa tcagattgct
    20281 ttgttaaaaa gtgatcgata tatttgaaat caagtttcgc atattgaaat tttaagccaa
    20341 aaaagcgatc aaaaaaacaa ggaagcctgg gatgaaagtg acatttgagc agttaaaagc
    20401 agcctttaat cgggtcttaa tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc
    20461 agagatgttc gcccgcacca ccgaatccgg cgtttattct cacggcgtta atcgtttccc
    20521 tcgtttcatt caacaactgg aaaacggcga tatcattcct gatgcccaac ccaaacgtat
    20581 aaccagcctc ggcgcaattg aacagtggga tgcccagcgt tcgatcggca acctgacggc
    20641 gaaaaagatg atggatcgcg ccattgaact ggctgccgat cacggtattg gtctggtggc
    20701 acttcgtaat gccaaccact ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa
    20761 aggctatatt ggcatttgct ggaccaactc catcgccgta atgccgccgt ggggcgcaaa
    20821 agagtgtcgc attggcacta acccgctgat cgtcgccatt ccttccacgc cgatcaccat
    20881 ggtcgatatg tcgatgtcga tgttctctta cggcatgtta gaagttaacc gcctggcggg
    20941 ccgtcagctc ccggtcgatg gtggctttga tgatgagggc aatttgacca aagaacctgg
    21001 cgttatcgag aagaatcgcc gcattttgcc gatgggctac tggaaaggtt ctggcatgtc
    21061 gattgtgctg gatatgatcg ctactctcct ttccgacggc gcatcggttg ccgaagtcac
    21121 ccaggacaac agcgacgaat acggcatttc acaaattttt attgccattg aagtggacaa
    21181 gcttatcgac ggtcccaccc gcgatgccaa gctgcaacgt atcatggatt acgttactac
    21241 cgctgaacgc gctgacgaaa accaggccat ccgcttaccc ggccacgaat ttactaccct
    21301 gctggccgaa aaccgccgta acggtattac cgttgatgac agcgtgtggg ccaaaatcca
    21361 ggcgttataa ggagataagt catgattttt ggacatatcg cgcagcccaa tccgtgccgt
    21421 ttgcccgccg ccattgaaaa ggggctcgat tttctgcgcg ctaccgattt caacgccctg
    21481 gagccgggcg ttgtcgaaat cgacggcaaa aatatttatg cgcaaattat cgatttaacc
    21541 acacgcgaag cggtggaaaa ccgtccggaa gtgcatcgtc ggtatatcga tattcagttt
    21601 ctggcgtggg gtgaagagaa aatcggcatt gctattgata cgggaaataa taaagtcagc
    21661 gaatcactat tagagcagcg cgatattatt ttttatcatg acagtgaaca tgaatcattt
    21721 atagaaatga taccgggcag ctacgcaatt ttctttccgc aggatgttca ccgaccaggt
    21781 tgtattttgc aaacagcctc tgagatacga aaaattgtgg ttaaagtcgc attaacggcg
    21841 ctgaattaat cgaataatcg tctacgccag aacgactggg taatgttatt gctctaacta
    21901 tattaataat ttaggttgct gcacatgcaa cttgaattat gacagacata gcctcaagga
    21961 atagctatga aaaaaatact tgaagcaata ctggcgatta atctcgccgt actttcctgt
    22021 attgtgttta taaacattat tttaagatat ggttttcaga caagcatttt atctgtagat
    22081 gaattgtcac gttatttatt tgtctggtta acatttattg gcgcgattgt ggcttttatg
    22141 gataacgccc acgttcaggt cacctttctg gtggagaaac tctcccccgc atggcaacga
    22201 cgagtcgctc tgggtaccca ttccttaatc ttatttattt gtggcgcact ggcctggggc
    22261 gcaacgctaa aaacaattca ggactggagc gattattcac cgatcctcgg tttacccatc
    22321 ggcctgatgt atgccgcttg tctgcccacc agcctcgtta tcgcattctt tgaactgcgt
    22381 catttatatc aactcatcac gcgtagcaat tctctaacgt cgccaccgca aggagcttaa
    22441 tcatggctgt gctgattttt ctgggctgtc tgttgggtgg tattgctatc ggtttgccta
    22501 ttgcctgggc actgttgttg tgcggggcgg cgttaatgtt ctggctggac atgtttgatg
    22561 tccagatcat ggcacaaacg ctggtgaacg gagccgatag cttctccctg ctggcgattc
    22621 cgttctttgt gctggcgggt gaaatcatga atgcgggcgg cttgtcaaaa cgcattgttg
    22681 acctgccgat gaaactggtg gggcataaac caggcgggct gggctacgtc ggcgtgctgg
    22741 cagctatgat tatggcgagc ctttctggtt ctgccgttgc ggataccgcc gccgttgccg
    22801 ctctactggt gccgatgatg cgcagcgcca actacccggt caaccgggcg gcggggctga
    22861 ttgcctctgg cggcattatc gcgccaatta ttcccccttc cattccgttt attatcttcg
    22921 gcgtttccag cggattatcc atcagtaagc tgtttatggc gggcattgcc cctggcatga
    22981 tgatgggcgc aacgctgatg cttacctggt ggtggcaggc cagtcgcctt aatttacccc
    23041 gccagcaaaa agcaacgatg caggaaatct ggcactcttt tgtctccggt atctgggcgc
    23101 tgtttcttcc agtcatcatt attggcgggt ttcgctccgg tctgtttacc cctaccgaag
    23161 ccggggccgt tgctgctttc tatgcgctgt ttgtcgccac agttatttac cgtgaaatga
    23221 ccttcgccac actttggcat gtactcattg gcgcggcgaa aaccacctca gtggttatgt
    23281 ttctggtggc ctcggcacaa gtttccgcct ggctgattac cattgctgaa ctgccgatga
    23341 tggtttctga tttactgcaa ccgttggtcg attcaccgcg tctgctgttt atcgtcatta
    23401 tggtggctat cctgattgtc ggcatggtca tggatttaac gccgacggta ttaattctta
    23461 ccccggtatt aatgccttta gttaaagagg caggaatcga tccgatttat ttcggtgtca
    23521 tgtttatcat taactgctca atcggcttaa ttacaccgcc tatcggtaat gtacttaacg
    23581 ttatttcagg ggtggcaaaa ctcaaattcg atgatgcagt cagaggcgtt ttcccttacg
    23641 tcctggtttt atattcatta ttagtcgtat ttgtttttat tcccgatctc atcatcctcc
    23701 ctttgaaatg gattaattaa aaggaaaata ttatgaaatt acgctctgta acctacgcat
    23761 tattcattgc tggcctggct gcattcagca catcttctct ggcggcgcaa tctttacgtt
    23821 tcggttatga aacctcacaa accgactcgc aacatattgc ggcgaaaaaa ttcaatgatt
    23881 tattgcagga gagaaccaaa ggcgagctga aattaaaatt gtttccggat agcaccctcg
    23941 gtaacgcgca ggcgatgatc agcggcgtac gtggcggcac catcgatatg gaaatgtccg
    24001 gctcgaataa ctttgccggg ttatcaccag tgatgaactt gcttgatgtc cctttcctgt
    24061 tccgcgatac cgctcacgcg cataaaacgc tcgacggcaa agtcggtgat gatctgaaag
    24121 cctcacttga aggtaaagga ctgaaagtac tggcctactg ggaaaacggc tggcgcgatg
    24181 tcaccaactc gcgcgcaccg gttaaaaccc ccgccgacct gaaagggctg aaaattcgca
    24241 ccaacaatag cccgatgaat atcgccgcat tcaaagtctt tggcgctaac ccgatcccga
    24301 tgccatttgc cgaagtctat accgggctgg aaacgcgcac tatcgacgct caggaacacc
    24361 cgatcaatgt cgtctggtca gcaaaatttt tcgaagtgca gaaatacctt tctctgacgc
    24421 accacgccta ttccccgctt ctggtggtga tcaacaaagc gaagtttgat ggcttaagcc
    24481 cggagttcca gcaggcgcta atttcatctg cacaagaagc gggtaactac caacgcaaac
    24541 tggttgccga agatcagcaa aaaatcatcg acggcatgaa agaagcgggc gtggaagtca
    24601 tcaccgatct cgaccgcaaa gcctttagcg acgcactggg gaatcaggtt cgcgacatgt
    24661 ttgttaaaga tgtgccgcag ggagctgatc tgctgaaagc cgtggatgag gtgcaataaa
    24721 ccatgacgca atactggctg gggttagatt gtggcggtag ctggctgaaa gccgggctgt
    24781 atgaccgcga aggccgggag gcaggcgtgc agcgcctgcc gctgtgcgca ttaagcccgc
    24841 agccaggttg ggcagagcgc gatatggcag aactgtggca atgctgcatg gctgtcattc
    24901 gcgaactgct tacccattcc ggcgttagcg gggaacaaat tgtcggtatc ggcatctccg
    24961 cgcagggaaa aggcttgttt ttgctggata aaaacaacaa gccgctcggt aacgccattt
    25021 tgtcctcgga tcgccgggcg atggaaatcg ttcgtcgctg gcaggaagat ggcatcccgg
    25081 aaaaactcta cccgctgacc cgacaaacct tgtggaccgg gcatccggtg tcgctgttac
    25141 gctggctgaa agagcacgaa ccggaacgct acgcgcaaat tggctgcgtg atgatgacgc
    25201 acgactacct gcgctggtgt ttaactggcg tcaaaggctg tgaagagagc aatatttccg
    25261 agtccaacct ctacaacatg agtcttgggg aatatgaccc gtgcctcacc gactggctgg
    25321 ggatcggtga aatcaaccac gccctgccgc cggttgtcgg atctgccgaa atctgcgggg
    25381 aaatcaccgc tcagacagcc gtactgaccg gtctgaaagc gggtacgccc gttgtcggcg
    25441 gcctgtttga tgtggtttcc accgcactct gcgccgggat cgaagacgaa tctaccctta
    25501 atgcagtgat gggcacctgg gcggtgacca gcggcataac ccgcggttta cgtgacggtg
    25561 aagcgcatcc gtatgtctat ggtcgctacg ttaacgatgg tgaatttatc gttcacgaag
    25621 ccagccccac ctcttccggc aacctcgaat ggtttaccgc acagtgggga gaaatctcgt
    25681 ttgatgagat caaccaggcc gttgccagct tgccgaaggc cggtggcgat ctctttttcc
    25741 tgccgttcct gtacggcagc aacgccggac tcgagatgac cagcggtttc tacgggatgc
    25801 aggccattca tacccgcgcg cacctgttgc aggctattta tgaaggcgtg gtgttcagcc
    25861 atatgaccca tctcaaccgg atgcgcgaac gttttactga tgtgcacacc ctgcgcgtca
    25921 ctggcggccc ggcgcattcc gatgtctgga tgcaaatgct ggcggacgtc agcggtctgc
    25981 gtattgagct gccgcaggtg gaagaaaccg gctgctttgg tgcagcactt gccgcccgcg
    26041 tcggcaccgg ggtttatcgc gatttcagcg aagcccagcg tgatttacag cacccggtgc
    26101 gcaccctgct gccggatatg actgtccatc agctttacca acaaaaatac caacgctatc
    26161 agcatctcat tgccgcactt cagggctttc acgcccgcat caaggagcac atattatgag
    26221 ccgaccactt ctgcaactgg cactcgacca ctcatcactt gaagccgcac agcgcgacgt
    26281 gacgcagtta aaagacagcg tcgatattgt cgaagcgggc accattctct gtttaaacga
    26341 agggcttggc gcggtgaaag ccttgcgcga acagtgccct gacaaaatca tcgttgccga
    26401 ctggaaggtc gccgacgctg gtgaaacgct cgcgcaacag gcatttggcg caggcgctaa
    26461 ctggatgacc atcatctgcg ccgcaccgct cgcgacggtg gaaaaaggcc acgcgatggc
    26521 gcaacgctgc ggtggtgaaa ttcagatcga gctgttcggt aactggacgc tggacgacgc
    26581 ccgcgactgg catcgtattg gcgtgcggca ggcgatttat catcgcggtc gtgatgcaca
    26641 ggccagcggg caacagtggg gcgaagccga tctggcacgc atgaaggcgc tttccgatat
    26701 cggccttgag ctttccatta ccggcgggat tacccctgct gacctgccgc tgtttaaaga
    26761 tattcgcgtg aaagcgttta ttgccgggcg agcactggca ggctcagcaa atccggcgca
    26821 ggtggctggc gatttccatg cgcaaatcga cgctatctgg ggaggcaagc gtgcgtaatc
    26881 atccgttagg gatttatgaa aaggcgctgg cgaaagatct ctcctggccg gagcggctgg
    26941 tgctggcaaa aagctgcggt tttgattttg tcgaaatgtc ggtggatgaa accgacgaac
    27001 ggctttcacg tcttgactgg agcaccgcac aaaggacttc gctggttgcc gcgatgatcg
    27061 aaacgggtgt tgggatcccg tcgatgtgcc tgtccgcaca tcgtcgcttt ccttttggta
    27121 gccgtgacga cgcggtacgc cagcgagcac gggagatcat gagcaaagcg attcgtctgg
    27181 cacgcgatct cggcatacgc accatccagc tggcgggcta cgacgtctat tacgaagacc
    27241 acgatgaagg cacccggcaa cgttttgctg aagggctggc gtgggcggtc gaacaggcgg
    27301 cggcatcaca agtcatgcta gcggtggaga ttatggacac cgcgtttatg aactccatca
    27361 gtaaatggaa aaagtgggac gagatgctcg cctcgccgtg gttcaccgtc tacccggacg
    27421 tcggcaacct cagcgcctgg ggcaatgatg ttcccgccga actgaaactg ggcattgacc
    27481 gtatcgccgc gatccacctg aaagacaccc agccagttac cgagcaaagt cccggacagt
    27541 tccgcgacgt gccgtttggc gaaggctgcg tcgatttcgt tggcatcttc aaaacgctgc
    27601 ataaactgaa ctatcgcggt tctttcctga ttgagatgtg gaccgaaaaa gccaaagagc
    27661 cggtactgga gattattcag gcgcggcgtt ggattgaagc gcgtatgcag gaggctggat
    27721 ttatatgtta gagcaactga aagccgacgt gctggcggcg aatctggcgc ttcccgctca
    27781 ccatctggtg acgttcacct ggggcaatgt cagcgcggta gacgaaacgc ggcaatggat
    27841 ggtaatcaaa ccttccggcg tcgagtatga cgtgatgacc gccgacgata tggtggtggt
    27901 tgagatagcc agcggcaagg tggtggaagg tagcaaaaag ccctcgtccg acacgccaac
    27961 gcatctggcg ctttaccgtc gctacgccga aatcggtggc attgtgcata cccactcacg
    28021 ccacgccacc atctggtcgc aggccgggct ggatctcccc gcctggggca ccacgcacgc
    28081 tgattatttc tatggtgcca tcccctgcac gcgacagatg accgcagagg agattaacgg
    28141 cgaatatgaa tatcagaccg gcgaagtgat cattgaaacc ttcgaagaac gtggcaggag
    28201 tccggcacaa atcccggcgg tgctggtgca ttctcatggc ccgttcgcat ggggtaaaaa
    28261 cgccgccgat gccgtgcata acgccgtagt actcgaagaa tgcgcctata tgggcctatt
    28321 ctcgcgccag cttgcgccgc agctccctgc gatgcaaaac gaactgctgg ataagcacta
    28381 cctgcgtaag catggggcca atgcctatta cgggcagtaa tccctcacgc cggggcttca
    28441 tcgccccggc actacgaatt gatatgttcc ttgctgtaac gccgcttcca cgctgctggc
    28501 gttaaaccgg tgtgcttgcg aaaaatctgc cggaaatagc caacgtcatt aaaccgtatt
    28561 gcatggagca ggccgacaac gtcgagtcac tgcataacct gttgctgccc gccgatgcgc
    28621 catttacgac atttgaaggc aagggattgt tcagccataa gatcctgatt cagacgccag
    28681 gttcccggta tgaacagagc aatccagagc aacaaccttt gtggcattac gacggagcac
    28741 cagccgcacc gacatccacc ggtgaattaa aaacgccagc tcacgccggt gacaaaggta
    28801 aaactctcat tacgatcaat catcgggctg tcggtaatct cttccggcag ctcgctgtat
    28861 cctgcactcg ccatcaatac gacgtgctct cctatcgggt attttgccgt caggctgaca
    28921 tagggcaccc aggcatcctg cgcggaataa cttgccagac cgctacgacg ggactcactc
    28981 tctgaaatgc catagtaata gtcactgaaa ttctcgtcat aatagagcac gcccagcgca
    29041 ggtgtcagcg acaggcgacc tatctgcatt ttgtggaaaa tcgatagctc ccccacccag
    29101 ccgttgctgt tatccagaac gtccgcagcc gcagaggctt tcacagttcc ccaacgctcg
    29161 tggtgatacc aggcaacccc cgccatagcc gtactatcac gcttatcaag ctgttgcatg
    29221 gcataatcgt cattatcggc agggtcaaat tccagcggca tccaggatgc ggtcaggcta
    29281 agttcatttt tttcactttg cgacagaata aaacctaacg tggtctgacg aacataaaaa
    29341 gaatcacctt cataactaat caggggaatt gcctttgtat tttcattata accgcgataa
    29401 ggcgattcat tataagccgc accggcacca atggataatt cagaagcagt tgcgcttgcc
    29461 ataaaaggca acgcaaataa cgccacaata ttgcggttaa ttaacatgtt cggtattcca
    29521 ttttaaaata agataaaaaa gagtcggcat agtaatgctt acaatttctc cggtgcaaga
    29581 ttcatattta tatagtaata tataaaatta tatataattg ggctgttgcg atgacaaaat
    29641 tacaacttaa gtaccgggag ttaaaaatta tctcggtaat cgctgccagt gaaaatatca
    29701 gccatgccgc gactgtactt ggcatcgcac aggccaacgt cagcaaatat cttgctgatt
    29761 ttgaatcaaa agtgggttta aaagtctttg accgaaccac ccggcagctg atgctcacac
    29821 cttttggtac cgcgctgtta ccgtacatca acgacatgct ggacagaaac gagcagctta
    29881 ataattttat tgcagattat aagcatgaga aacgtggtcg ggtcaccatc tacgccccaa
    29941 ccggcataat tacctattta tccaaacatg taatcgataa aattaaagat atcggtgata
    30001 tcaccttatc gctaaaaacc tgcaatcttg agcgtaatgc cttttatgaa ggcgtcgaat
    30061 ttcctgatga ttgtgatgtt ttaatcagct acgctccacc aaaagatgaa tcgctggttg
    30121 ccagttttat tacccaatat gccgtaaccg cttatgccag ccagcgctat cttgagaaac
    30181 atcccattag ccgtcctgac gaactcgaac atcactcctg tattttgatc gactcgatga
    30241 tgatcgacga cgcgaatatc tggcgcttca acgtcgccgg gagtaaagag gtgcgagatt
    30301 atcgcgtgaa gggaaattac gtttgcgaca acacgcaatc tgcgctggag ctggcacgaa
    30361 atcacctggg gattgtgttt gcgccagaca aaagtgtgca gagcgacctg caagacggca
    30421 cgctggtgcc ctgctttcag caaccctatg aatggtggct ggatctggtg gctatcttcc
    30481 gtaaacggga ataccagccc tggcgggtcc agtatgtgct ggatgaaatg ctgcgtgaaa
    30541 ttcgccacca gcttgctcag tcgcagcaac tgcggcctga acaagcggcg gaaagcgaag
    30601 attaatgatc gagataaagg taatgcaccc agctggtcat gcgtaatagc actttgcgca
    30661 tcaccgagac atggctgaaa tgatcagagt aaaccgccac ctgggcgtaa ataccgtctg
    30721 gtaaagcgct caaatcagtg tgctcatcca gttcaatggt cgcgataacg ccatctgaac
    30781 ccggcgctgt gtttaacgtc tgtaaggtgc cggtcgactg ataagctccg ccgggaacgg
    30841 ctggactaat ggctgccagc ttaccgctga ataccttacc tggcagagca ttaaacacca
    30901 cttccgcatc gtcgccagga gccagtcgca gcaaggagtt ctgacggaac tgcgccacga
    30961 tttgtcgttt ctgatcgggt ataaacacca tcaccggacg tagcggcagc gacgcggcat
    31021 aggtacccgg acgaatcagt acctgggtca cataaccatc gctcggcgca cgcactatcg
    31081 tctgctcaag gttatatttt gcttccgcga gctgtgcttt caggctggcg atttgagaat
    31141 gttcacccaa caccaggcta tccagctggc tctggatctg tttttgttcc gccgccgatg
    31201 acttcacgga ggcttcctgc gccagataat tttgccgcgc cacatcgata tcgcgttctg
    31261 aaaacgggtt tactttcgcc tggctgccgc gtgcgtaacg ctgatactct ttagcgaatt
    31321 tatcccgcgt ggcctttgcc tgctgagtat tcgccgctat ctcatctaac tctgcgccca
    31381 acgcccgctg tttatgttct gcggtaacga tatccgccat cagccgatcc acccgcgcct
    31441 gataacgcgt cgggtccagt cgaaaaagca cctcgccttt tttaatcagc gtatttttct
    31501 tgtccgtcac ttcgatcacc acgcctgtca cctgcgggac aactggaata gaaataaccg
    31561 ctttttgcgc tttaaaggta tacggatggt tatagttcat taacagaatt aaaccgctga
    31621 caataaatat gccacccagg gccgcggtgg gaatggtcca tttatttaca ggaattttga
    31681 agatcttaaa cattgcccat gcgaaagcca cataggttaa aataatcagt aaatccataa
    31741 gtattactcg gcagaagagg atttgatgtc ggcgagctgt ttctccagcc cggcaatacg
    31801 ctgttgtagt tgcacaacgg aggagtcatg gttttgcatt ccccatcccc gctccgggcg
    31861 gtaaagcgtg gcccaaatcc acaaaaatgg ccagataaca tgtaacgtaa aaagactcac
    31921 ccaaccagca acatgaatgg cgtcggcatg aggatgatta cgttttttag caatcaggta
    31981 gggaatatca tgtaaaataa taatcccata gaatatcacc agaaatacaa aaataagcac
    32041 tcccagtgca aaatagtcca ggaacatatt tccctcaaag aatataaaaa agaacaatta
    32101 acgcatatta tgaaatgcca tgatgcagtg caatttcctt atttatataa cgacatattg
    32161 ttttttatat actttcttaa atgtgctgtc tggtttttca acaggacgag gtttatcata
    32221 ttgaaatagt ggaactattg gccaaactaa tgaataactc cagtaaaaca tccacagtac
    32281 agattaagcg tattaaacct tcaattatct accgtttatt gctgattggc ctcggatcac
    32341 caatggtgat ttacggcctg gttcgcccgc tcaccatcga aacgcgagat taaactcctg
    32401 acaaaacacg ccccagaaac gcacttctgt tggcgtagtc tgggttattg cgcacgtagg
    32461 tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc gtgtacgcct
    32521 tatccggcct acggtcaggt tcccgtaggc atgataagac gcgtaagcgt cgcatcaggc
    32581 aatgaatacc caatgcgacc agcttcttat atcagaacag ccccaacggt ttatccgagt
    32641 agctcaccag caggcacttg gtttgctggt aatgctccag catcatcttg tgggtttcgc
    32701 gaccgatacc tgattgtttg tagccaccaa acgccgcatg tgccgggtaa gcgtgataac
    32761 agttggtcca cacgcgccca gcctgtatgc cgcgccccat cttataggcc agattaccgt
    32821 tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc tccagcgctt
    32881 cttccatcgt tttgaaggtg gtcaccgcca gcaccgggcc aaaaatctcc tcctggaaca
    32941 cccgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg tctttcagtt
    33001 caccttccag cagcttgcgc cgcccgcctg tgagcacgtc agcgccctct tttttaccga
    33061 tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgcg cccatttgcg
    33121 tcacgctgtc gagtgggtta ccgctgcgaa tgctttcgac acggcggatg gcgcgttcca
    33181 taaagcgttc gtagatagac tcctgcacta aagcacggct tggacaggtg caaacttcgc
    33241 cctggttaaa ggcgaacagt gcaaagcctt ccagcgcttt atcgaaaaag gcatcttctt
    33301 catccatcac atcagcaaag aagatatttg gcgacttgcc gcccagctcc agggttaccg
    33361 gaataatgtt ttgcgtggcg tattgcataa tttgttggcc cacttccgtt gagccggtaa
    33421 acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc ccacctgcgc
    33481 cgttgaccac gttcaccacg cccggcggca gtaaatcacc gacgatttcc attagcagca
    33541 gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac acagttgccc gccgccagcg
    33601 cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata atctgcccca
    33661 ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatcaact tcactgatcc
    33721 caccttcctg cgcccgaata cacgaggcga aatagcgaaa atggtcaatc gccagcggca
    33781 cgtcggcagc actggtttcg cgaatgggtt tgccgttatc ccaggtttca gccgtcgcta
    33841 acagctcaag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc gcgcgatcct
    33901 gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc agcatccagc gccagatcga
    33961 tgtctcgttt gcccgaagac gccacttcgc acagcagctg cccggtcacc ggcgtcagat
    34021 tctggtaata ctcgccgtcg gcaggggcta cccattcgcc gccaataaag ttgtcatagc
    34081 gggcttttaa cttgaggggg aaaccatact cgccgggctt aatctgtgct gaagggggat
    34141 tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg tgactggcga
    34201 aatcttcgcc agtcggtaac agctttacga cagctatcac gaatttacgg gcaagggtta
    34261 cagagatagt tgaaaaagcg gctgacaatt tgccagccgt tgtggaaatg atgattacat
    34321 cgctgcgcga taaatcgcca caatttcttc gtgagttgcc tggatcgggt tagtaaaacc
    34381 acaggcatct ttcagggcat tagtcgccag aaccgcgaaa tcttcttctt tcacgttcag
    34441 gtcgcgtagg cctgccggga tatccacttt cttcgccagt tcacggatgg cgttaatgca
    34501 ggcttcagca ccttccgcat cgtttttacc tgtcacgttc acgcccattg cagcggcaca
    34561 gtcacgcaga cgtgcggcgg cgactttgct gttgaatacc tgaacatgcg gcagcaaaac
    34621 ggcgttacat acaccgtgtg gcaggttgta gaaaccgccc agctggtgcg ccatcgcatg
    34681 aacataaccc agagaagcat tattgaacgc cataccagca aggaactggg cataagccat
    34741 tgcttcacgc gctttcgcat tactgccatc ttcaacggct aacggcaggt tttcggcaat
    34801 catggtcacg gctttcagtg cacaagcgtc agtgatcggc gtggcggcaa tagaaacata
    34861 tgcttcgata gcgtgcgtta aggcatccat acccgttgcg gcggtcagtg acttcggcat
    34921 accaatcatc agagaggagt cattgacaga aagcagcgga gtgacatgtt tatcaacaat
    34981 cgccattttg atatgacgcg cttcgtcagt gatgatgcag aaacgggtca tttcagaggc
    35041 cgtacccgca gtggtgttga tggcgatcat cggcagctgc ggttttgcag agcggtcaac
    35101 gccttcgtaa tcgcgaatat cgccgccatt ggctgccacc agcgcaatac cttttgcgca
    35161 gtcgtgtgga gaaccaccgc ctaaggagat cacgctatcg caattatttt ctttaagtaa
    35221 tttcaaacct gcggcgacgt tttccgtggt ggggttaggt tgggtgccat cataaataac
    35281 gctaaaaata ttgcgttctt ccagtgcttt ttgcacatca cccgccatac ctaatttcgt
    35341 taacatactg tcagtgacaa ttaaggtacg ggtaaatcca taatctgcca tcatattcat
    35401 tgcatcagtc aatgaatcag cgccgatgac attcacagaa ggaataaaga aagttgaaga
    35461 tgccataaca cactcctgaa agtgtaaaaa agggtgggca gcttactgag gattttcatt
    35521 cgaaaacatg atcattatta ttaattatca aaatt
//
LOCUS       DAZFFI010000040        35410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFFI010000040 DAZFFI010000000
VERSION     DAZFFI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.040"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..1086
                     /locus_tag="ACHJ12_002286"
     CDS             151..1086
                     /locus_tag="ACHJ12_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4990063.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGGNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1142..1684)
                     /locus_tag="ACHJ12_002287"
     CDS             complement(1142..1684)
                     /locus_tag="ACHJ12_002287"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4990064.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1668..1979)
                     /locus_tag="ACHJ12_002288"
     CDS             complement(1668..1979)
                     /locus_tag="ACHJ12_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4990065.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2164..3054)
                     /gene="cyoE"
                     /locus_tag="ACHJ12_002289"
     CDS             complement(2164..3054)
                     /gene="cyoE"
                     /locus_tag="ACHJ12_002289"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4990066.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3066..3395)
                     /gene="cyoD"
                     /locus_tag="ACHJ12_002290"
     CDS             complement(3066..3395)
                     /gene="cyoD"
                     /locus_tag="ACHJ12_002290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4990067.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3395..4009)
                     /gene="cyoC"
                     /locus_tag="ACHJ12_002291"
     CDS             complement(3395..4009)
                     /gene="cyoC"
                     /locus_tag="ACHJ12_002291"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4990068.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3999..5990)
                     /gene="cyoB"
                     /locus_tag="ACHJ12_002292"
     CDS             complement(3999..5990)
                     /gene="cyoB"
                     /locus_tag="ACHJ12_002292"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4990069.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6012..6959)
                     /gene="cyoA"
                     /locus_tag="ACHJ12_002293"
     CDS             complement(6012..6959)
                     /gene="cyoA"
                     /locus_tag="ACHJ12_002293"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4990070.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7419..8894)
                     /gene="ampG"
                     /locus_tag="ACHJ12_002294"
     CDS             complement(7419..8894)
                     /gene="ampG"
                     /locus_tag="ACHJ12_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4990071.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8938..9516)
                     /gene="yajG"
                     /locus_tag="ACHJ12_002295"
     CDS             complement(8938..9516)
                     /gene="yajG"
                     /locus_tag="ACHJ12_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4990072.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9821..10138
                     /gene="bolA"
                     /locus_tag="ACHJ12_002296"
     CDS             9821..10138
                     /gene="bolA"
                     /locus_tag="ACHJ12_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4990073.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10482..11780
                     /gene="tig"
                     /locus_tag="ACHJ12_002297"
     CDS             10482..11780
                     /gene="tig"
                     /locus_tag="ACHJ12_002297"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4990074.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            12026..12649
                     /gene="clpP"
                     /locus_tag="ACHJ12_002298"
     CDS             12026..12649
                     /gene="clpP"
                     /locus_tag="ACHJ12_002298"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4990075.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12775..14049
                     /gene="clpX"
                     /locus_tag="ACHJ12_002299"
     CDS             12775..14049
                     /gene="clpX"
                     /locus_tag="ACHJ12_002299"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4990076.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14237..16591
                     /gene="lon"
                     /locus_tag="ACHJ12_002300"
     CDS             14237..16591
                     /gene="lon"
                     /locus_tag="ACHJ12_002300"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4990077.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16782..17072
                     /gene="hupB"
                     /locus_tag="ACHJ12_002301"
     CDS             16782..17072
                     /gene="hupB"
                     /locus_tag="ACHJ12_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4990078.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17264..19135
                     /gene="ppiD"
                     /locus_tag="ACHJ12_002302"
     CDS             17264..19135
                     /gene="ppiD"
                     /locus_tag="ACHJ12_002302"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4990079.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAASLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19286..19657
                     /gene="ybaV"
                     /locus_tag="ACHJ12_002303"
     CDS             19286..19657
                     /gene="ybaV"
                     /locus_tag="ACHJ12_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4990080.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19751..20149
                     /gene="fadM"
                     /locus_tag="ACHJ12_002304"
     CDS             19751..20149
                     /gene="fadM"
                     /locus_tag="ACHJ12_002304"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4990081.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20201..20896)
                     /gene="queC"
                     /locus_tag="ACHJ12_002305"
     CDS             complement(20201..20896)
                     /gene="queC"
                     /locus_tag="ACHJ12_002305"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4990082.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20961..22661)
                     /gene="ybaE"
                     /locus_tag="ACHJ12_002306"
     CDS             complement(20961..22661)
                     /gene="ybaE"
                     /locus_tag="ACHJ12_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4990083.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQAVTVNELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22761..23579
                     /gene="cof"
                     /locus_tag="ACHJ12_002307"
     CDS             22761..23579
                     /gene="cof"
                     /locus_tag="ACHJ12_002307"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4990084.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHCDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23732..24190
                     /gene="decR"
                     /locus_tag="ACHJ12_002308"
     CDS             23732..24190
                     /gene="decR"
                     /locus_tag="ACHJ12_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4990085.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24220..25992
                     /gene="mdlA"
                     /locus_tag="ACHJ12_002309"
     CDS             24220..25992
                     /gene="mdlA"
                     /locus_tag="ACHJ12_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4990086.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            25985..27766
                     /gene="mdlB"
                     /locus_tag="ACHJ12_002310"
     CDS             25985..27766
                     /gene="mdlB"
                     /locus_tag="ACHJ12_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4990087.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYLQMYQLQLAGEELAASVREEESLSA"
     gene            27947..28285
                     /gene="glnK"
                     /locus_tag="ACHJ12_002311"
     CDS             27947..28285
                     /gene="glnK"
                     /locus_tag="ACHJ12_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4990088.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28315..29601
                     /gene="amtB"
                     /locus_tag="ACHJ12_002312"
     CDS             28315..29601
                     /gene="amtB"
                     /locus_tag="ACHJ12_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4990089.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29650..30510)
                     /gene="tesB"
                     /locus_tag="ACHJ12_002313"
     CDS             complement(29650..30510)
                     /gene="tesB"
                     /locus_tag="ACHJ12_002313"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4990090.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30728..31300
                     /gene="ybaY"
                     /locus_tag="ACHJ12_002314"
     CDS             30728..31300
                     /gene="ybaY"
                     /locus_tag="ACHJ12_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4990091.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31331..31642)
                     /locus_tag="ACHJ12_002315"
     CDS             complement(31331..31642)
                     /locus_tag="ACHJ12_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4990092.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31805..31901
                     /gene="ffs"
                     /locus_tag="ACHJ12_002316"
     ncRNA           31805..31901
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ12_002316"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            32021..32374
                     /gene="ybaA"
                     /locus_tag="ACHJ12_002317"
     CDS             32021..32374
                     /gene="ybaA"
                     /locus_tag="ACHJ12_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFO4990093.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32416..33966)
                     /gene="pdeB"
                     /locus_tag="ACHJ12_002318"
     CDS             complement(32416..33966)
                     /gene="pdeB"
                     /locus_tag="ACHJ12_002318"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4990094.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(34130..34600)
                     /gene="ylaC"
                     /locus_tag="ACHJ12_002319"
     CDS             complement(34130..34600)
                     /gene="ylaC"
                     /locus_tag="ACHJ12_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4990095.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34716..35267)
                     /gene="maa"
                     /locus_tag="ACHJ12_002320"
     CDS             complement(34716..35267)
                     /gene="maa"
                     /locus_tag="ACHJ12_002320"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4990096.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
BASE COUNT         9034 a         8575 c         9452 g         8349 t
ORIGIN      
        1 aataaacgat ttggtgacat ggatgataat gtttcaggct aaatgtgtgc ttattacttg
       61 gacctttatg gcactgtatt tattcattaa atggatattt ggtggttaat tattatggaa
      121 atatatgaca gtgaggacat aaaggtatac atgttaacta aaagaatatt attttctgta
      181 atgctgatag tttcaccaag cgtggtggcc agcgagaaag cgcatgaatt gtatgacagc
      241 atatacggtg gtaaacctgc tcccgatgtt ataaatacac tgcataaaat ggctgaatcg
      301 ggagatatcg acgcgcagag cctgttaggc tgggaatatt atcagcctcg ttatgatacc
      361 aaacctgatg ttcaggaagc gattaaatgg ttcgagttag cggctaaaca aggtgacaga
      421 gaagctccgt tagcgctggg gggtatctac tacgacggtg agcaggtgcg ggtggattat
      481 gccaaagcgt atgcactatt taatcaggcc gcgcagcacg gtgggaattt agcatggtcc
      541 aggttgggca ttatgtacgc caatggtcag tatgttgagg tagattgtaa gaaagcgaag
      601 gaatatcttg ataaaggtgt ccacatttat ggtggcccag aagactttct ggctacttgt
      661 cgaaaagaca tgattgacag aaaaacggtt gacgatacgt tacccgtgat tacggttacc
      721 cgttctggga tgcgagataa ttttttagat aaaggttttt cctgcatgga tagcctcttc
      781 gccaccacca ataaattagg cgaagtggcc aatcttcgcg tgacattcag cattcgtcgc
      841 ccatccggta aggagattaa ccaaacggtt ggcttcgcgc ctttcggttt aaaccggctg
      901 aatattagct ttactgatta cctatttggt tcatttacca gtaattcgtc tcttattctt
      961 tataaaccgg aatttgagcg aaaatcttgt gcaactgtaa ggaccacaat cgttgccgca
     1021 acggcaacca ttaacggcaa ggatgtggag ttgctgaaag cgggggcaat tgaacaaaag
     1081 tggtaacgct gctcagcccc cggctgaggc aaaacttgcc ctggcgggtg gcgataataa
     1141 tctaagccgc gagaatatgc tgaatcgcca aaaacatagg tacagcacca aaagcatttg
     1201 gtgtcgctga aagctgaaca aagccgagac gagcataaaa attaatggcg gcagggtcag
     1261 catcaagata aaccccttta attggtaatg cctggtgaat tatttttaca tgttcaaaaa
     1321 aatcacatag taggtcctga ccaaagcccc gcttttgata cttccgtgct acccccaaca
     1381 tgactaatct gacaacacca atttctgaag gttgtgaacc ctgaaggacg ccagaaacgc
     1441 gttgtttttc cagcgaatat gccgtaaaag tacagatgcc gatcagttca ccactttgtc
     1501 tgtcaataag tgctttagcc gcacagtcgc tattacgcac acttttcttt agcgatgcgc
     1561 gtacaaattt atcgataaca gggttaccgc aatcaaactg tttttgccca ggataagtaa
     1621 tatcagactg ataattacag gagaggacta tgggcagagt aatctcttca tgcttgtcga
     1681 ccatcgctgt ttttcctttt cattaacgcc ttgagggcga gcgtcggttg agcaggttca
     1741 gcgatgagtt ggttcatcag ttcccagctt tgattcgaaa gctcacggcg acgttggtta
     1801 tcaagcacac tttcggcgcg ttccatagca gcgctcaaaa taaaagcgct taaatctacc
     1861 ccaacggctt gcgcagcttc acgaagtttg tttttggcgt cgggactagt tttgagttcg
     1921 acccgcgcat ttttcagcgt tttatcgaca tcgggtagag tggctgtttt tccaggcatt
     1981 atgacctctt tgattaactg aatgaaatcg tagggagtcg atctcataca taatacggct
     2041 atcaaccgta tgattaattt atgttcatct gctcactctg tcaagatata cggttattag
     2101 ccgtatctct gatctcagac aataaaaaag gtgccgtagc acctttttaa tagagaggtt
     2161 ttgttaccac acagcagcca gcagcgtatg cgagtccggt accataaaat caacggacat
     2221 catcaccgag agggcagtga tggcgatgat agagaagccg aacagcttgc gcgcccagat
     2281 tctgtcatca gcaactttat aaccgcgcag agccatacct aaccaccaga cgctaaccgc
     2341 cgcggcgacc accagatatt tatatccagc gtaaccgcca agagagagca tcagcgtggc
     2401 aacggcaaag gcgatgatat acagcgtgat gtgattcttc gccaccgaaa tgccttttac
     2461 cactggcaat accggaatgt ttgccgcctg gtaatcctta aagcggaaaa tggcgatggc
     2521 ataggagtga ggcatctgcc acaggctgaa gatagccagc aggatcgctg cgccgctatc
     2581 gaactcgccg gttactgcac agtagccaat caccggcggc gcagcgccgg agagcgaacc
     2641 aatcaatgtg ccgtagactg agtggcgttt catgtacagg ctatagacgc cgacataaac
     2701 cacaaagccc atcaccccca gccagcaggc cagcggattc gcgccaaacc acagcagcat
     2761 aaagccagca atacccagca aggtggcgta caccagcgag acagcaggag agatcaggcc
     2821 tttaaccagc acccgattct tcgtcctttc catctttctg tcgatatccc tgtcgatgta
     2881 gttgttaaac acacaacccg acgccacaac cagtgacacc ccaaccagcg tgtagataaa
     2941 caggggataa tcaatgctgc cctttgaggc cagcaggaat cccccaatca ccgagatcag
     3001 gttgccaaag atgatgcctg gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc
     3061 cgctcttagt gcatcatcat gttgtagttg aggttccaca taatccagat ggagcctaca
     3121 accaggatag cgatgattag cacggtgaag acaaacgccg tcatgttcca gccttcatct
     3181 gatttggtat tcatgtgcag gaagcacacc agatgcacca gaacctgtac cactgccatt
     3241 gccaggattg ttcccagaat tacggccgga gaggcagccc ctgtcatcac catccagaac
     3301 ggaatcaccg tcagaatgat cgacaggata aagcctgtca tgtaggtttt tacgctgcca
     3361 tgggacgcgc cgctgtgatc ggtagaatga ctcattacat cgcccccatc agataaacaa
     3421 cagtgaacac acagatccaa accacatcca ggaagtgcca gaacaggctc aggcacatga
     3481 tgcgggtacg gttagtgctg gtcaggccgc gacgagcgat ttgtaccatc agcaccgcca
     3541 tccagataag accggaagtg acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg
     3601 acaggaagcc gctgcgatcc ggacccatgc cgttaacaat caggtgatgg aattcgtaga
     3661 tttccatccc gataaatccg gcaccaaaca accaggtcaa cgccagccag gagataacct
     3721 ggcttttgtt gtttttgtac atggcgatag ccgccatgcc gtaggtgatg gagctgaaca
     3781 acagcaagaa agtttcaacc agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc
     3841 cgcctgcggt gccgttcacc agaacggcat aggtagcaaa caagattgag aacagaatgc
     3901 agtcgctcat caggtagatc caaaatccga agattttggt tccgcctgca tcgtggtgcc
     3961 cgtgttcgtg cgcgtgggca gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct
     4021 gccttagtaa tctcatcgaa atgctggttt tccagttttt cgatttctgc caccggcacg
     4081 tagtaatcca cgtcctcgtc gaagcttttc acgatccagg tgatgatcat gcctgcgaag
     4141 ccaacaatcg ccagccacca gatatgccag atcatggcga aaccgaagat ggtggagaaa
     4201 gctgcaatga cgatacctgc accgctgttt ttcggcatat gaatttcttc atagtggtca
     4261 ggctttttat acgcttcgcc tttctctttc atttcccaga atgcatcacg ctcgtgaacg
     4321 tgcggcacaa cggcaaagtt atagaacgga ggcggggaag aggttgccca ctccagcgta
     4381 cggccacccc acgggtcgcc agtcaggtca cggttctggt cgcggtcgcg aatagaaacg
     4441 tacatctgaa taacgaggca gagaataccc agcgcaatca gtactgcacc gctagctgca
     4501 atcatcagca tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc
     4561 atgaagccca gcgcatacag cggcataaag gcaacgaaga agccgatgat ccagaaccag
     4621 aacgcgcgtt taccccaggt ttcattcagt ttgaaaccga aggctttagg ccaccagtag
     4681 gtcatccctg cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca
     4741 atcaggaaca ggctgttatg cagaacgaag tccgcgcccg gtactgccag cagcacgcca
     4801 gtcatcccgc ccaccgagaa agtgacgata aaaccgatgg tccacagcat cgcagaatgg
     4861 aacacgatgc ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg
     4921 atggcgataa tcattgtggt gataccaaag aaggcgttta cgttcgcgcc cgcacccatc
     4981 gtaaagaagt ggtgcagcca aacgatgaac gacagcacgg tgatacagac ggttgcccat
     5041 accagcgagg tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac
     5101 acaccgaaaa caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg
     5161 ttgatgtaca tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag
     5221 cgatccaggg tcaacaacgc gacggtaacc gtcagaattg ggaaggaagc aataatcagt
     5281 acgttcgcgc acagtgatgc ccaggtaaat actggcatct tgaacatggt catgcccggt
     5341 gcgcgcatct tcagaatggt aacgaagaag ttgataccgg taagcgtcgt acctataccg
     5401 gatagctgga gactccatat ccagtaatcg acaccgactc ccggactgta ctctattccc
     5461 gatagcggtg gataggccag ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg
     5521 ttaaccagaa tcacaccaac aacagtaaac cagaagctta agttgttgag gaacgggaac
     5581 gcaacgtcac gcgcgccgat ctgcagcgga accaccaggt tcatcagacc gataacgaaa
     5641 ggcatcgcca cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg
     5701 tgaggtggca ggaagcccgc ttcgcccgcc gaggcaagag cctgctggct acgcatcata
     5761 atggcgtcag caaaaccacg cagcaacatc acaatcgcca cgatgatata catgataccg
     5821 aggcgtttat ggtcgacgga ggtcagccac tctttccaca ggtaggtcca cttaccgaag
     5881 taagtgatca ggccaacgag cgccagacct cccaaaataa tgccagcgat cgtaaccatg
     5941 acgataggtt catggaacgg gactgcatca agtgataatt ttccgaacat ctttattctt
     6001 cctcaacccc tttaatgggc ggattccgcg tggctcatgt ccatgccttc cataccttcg
     6061 tgtgcactgt gctcaccttc tggctgggtc atgtccatgc tcttaccgtg agccataaac
     6121 ttgttaatca catcggcaaa caagtctggt ttcacgttgg agaaatattc cacctggttg
     6181 tattcgctag gcgcggccag tttttcgaac gcagccatgt cagacatggt gttcggcgac
     6241 tgtttcgctt ttgcgaccca ttggtcgaat tcggcgcgat ccggtgttgc aatagctttg
     6301 aacttcatgc ctgagaagcc cggcccgctg tagctggcgg agataccgtc ataagtaccg
     6361 ggttcgttgg cgatcagatg caggcgagtc tgcataccgg ccatggcata aatctggcta
     6421 cccagacgcg gaatgaagaa ggagttcatc acggagttgg aggtcacttt gaagtacacc
     6481 ggagtgttcg ccgggaaagc gatttcattc acggtagcaa tgccctgttc cgggtagatg
     6541 aagaaccatt tccagtccat ggaaaccact tcgatggtaa tgggcttctc gtcgtgtgcc
     6601 agcggcttgc taggctcaag agcgtgagtg gttttccagg tcagtaccgc aaggaagatg
     6661 atgattaaga taggtaccgt ccagaccaca gcttccactt tattggagtg tgaccagttc
     6721 gggctgtact tagcatcttt attgctcgca cgatacttcc aggcgaaacc aacagccatc
     6781 aagattgcgg gaataacgac aatcaacatc aggccaaatg ccgtcagtat cagtgaacgt
     6841 tgctccagac caatctgtcc tttgggatct aacagcgcag aattacagcc actgagcaat
     6901 acagtgcctg caaataatga caaccatccc aaacttttat tgtatttcct gagtctcatt
     6961 taacgacctc aattccacgg gatctggtgg cgtttaaagt gtgtgggcat tttacgggaa
     7021 ggttacatta ctgtaaacat gattaaattc ctgttacttg gatttggctg tcaggtcaca
     7081 tatgttgcaa aacatatcaa gttgatttta agaagctgtt gcaaaagggc gttataacga
     7141 aaggggagtg cagaaggagt ttggacgggc aattggtata accaatctgg aataaaacaa
     7201 ttgtttaaca aataattatc attatggtga caattgggtg ggggaaaata aagactcatt
     7261 agctgaatcg tgtaagaaaa aatagaattc ataaattgcg tcaataattt ccaaacgcta
     7321 agccgcacaa aagaacaaat attattaagc gatactgatg tttatttagc cgtaataatt
     7381 acggcgagtg attactacag ctaaataata tttacagatt acgtcagatg cgtttttcgt
     7441 agcgccagat aatccagcaa accaccaagc acgacgccag aaagggcgac taaaaccccg
     7501 acttccagca gagcaggcag gaaagagaag tgcgtcaaat ccagcgcgtc catcgtcaac
     7561 agcagtaacc acacggccaa caggctgacg cccgccgcca gtgtccacat ggcaaaggca
     7621 taacctgccg gatattcggt acgggagata aagttgtcat ttactcgtgt atattcaagc
     7681 gtctggcggc aaaccagcag caaaataagc cccggtacgg cagcggcgac ggagaataga
     7741 tagaatgtcg accagccgtg tgcttcaaca aaccaacccg ccacggggcc gacataaact
     7801 cgccctacag cggaaagcgc tgagagcagg gcaaattgag ttgcggaaaa tgacttatta
     7861 cacagcgtca ttaacagcgc gacaaaggct gatgtgccca tcccgccaca gaggttttcg
     7921 aaaaagacgg ctgcgcccat gctgtagaga tgcttatcag taatcgacag cagccagtaa
     7981 ccagcgttag acgcaccttg taaaatgccg aaaatcagca gcgcccggaa cagcgacagg
     8041 cgctgcatca aaatcccacc gtacaatgcg cccacaatgg tcgccagtaa gccaagcgtt
     8101 ttgttaacca cgcctacttc acccgcatca aacccgacgc cgcgaatcaa aaacgtggtt
     8161 gtcaggctca tggcgaatgc gtcgcccagc ttatacagca cgataagaag caaaataagc
     8221 caggcattat tgcgaccaaa gaaatctcgc agaggtgcaa caaccgcttg ttccagcgtt
     8281 tttggcacag gaatggtgtc ggtcggttct ggtgcaagca acgtcgcgat aatacaaggg
     8341 atcaacagcg ccgccatcaa ccagtacatg ccctgccagc ccagccattt atctgccagc
     8401 cacagggcta agccgccgga aaccagcatc cctaaacggt aacccagcac gctgattgcc
     8461 gcgcccgcac cacgttcttc tgccgggagc acatcggttt tccacgcatc gaagacaata
     8521 tcctgggagg ccgagcaaaa agcgatcacc accgccagtg ccgccatcca gcggagttgg
     8581 gtgccaggtt cgagaaaacc catcgccgca atggcgacta ataacaggat ttgcgtggcg
     8641 agcagccaac cgcgccgccg cccaaaaaat gggggcgtgt agcggtccat cagcggtgac
     8701 cagaggaatt taaaaacgta agcctggcct accagagaga agaaaccaat ggttttgaga
     8761 tcaatattct cgaccgtcat ccaggcctgt aaggtgccgg atgtcagggc gagcggtagc
     8821 ccggaagcaa aacccaggat cagcaatatg gctgaacgcg gctgttgaaa aatacgtaaa
     8881 tattgactgg acatgggcgt ctacaggccc ggtttgcacc gggccagagg gcagaaatta
     8941 acgcgcgttc tgcttgataa attcgtgaat gctggtgtcc tgagacatat cagcgatggt
     9001 atcggtcagc acactgttaa ccgcatcggc gatatttttg ttggaggcct ggaacgcacc
     9061 ttcaacgttg tagctggcac gatagttttt ggtcattttg ttgccattct gcgcggtagc
     9121 gatgatggcg atatccgctt tggtagcgat gttgtagcgc acgttgccct gggacacgtc
     9181 agcatacagt tggctaacga tgatttgcag attaaccggg ccattcggac caaccatgta
     9241 accacgcgcg gtcatctgtt tttccagcac ttcttgcagc aggaaacgca gatcgcggga
     9301 ggcggtcagg gtaacgattt gattatcgcg ggtgactttt gccagcgcct gatcggtacg
     9361 ctgatcggca ccattaatgc ttacggtgac gcccatcagg cttggatcct gctgtggcag
     9421 tgtaatcgtc ggggaaactt caatagttgt tggcggtttt gcgcatcctg caagcataaa
     9481 cagagcaact aacgggaaga ggattttttt gaacatgttc gggctctcag agactcttaa
     9541 gcgtgtttgg taaaaattcc cgccatcata acattgccaa cggcgagggg aagtgggtaa
     9601 ggcatgtaaa ttcatcatgt tgacgaaata atcgcccctg gcaaaagaaa cactgatgcg
     9661 aggcctgtgt ttcaatcttt aaatcagtaa acttcatacg cttgacggaa aaaccaggac
     9721 gaaacctaaa tatttgttgt taagctgcaa tggaaacggt aaaagcggct agtatttaaa
     9781 gggatggatg acatctcagc gttgtcggag gagatatttc atgatgatac gtgagcggat
     9841 agaagaaaaa ttaagggcgg cgttccaacc cgtattcctc gaagtagtgg atgaaagcta
     9901 tcgtcacaat gtcccagccg gctctgaaag ccattttaaa gttgtgctgg tcagcgatcg
     9961 ttttacgggt gaacgttttc tgaatcgtca tcgaatgatt tacagtactt tagcggagga
    10021 actctctact accgttcatg cgctggctct gcatacttac actattaagg agtgggaagg
    10081 gttgcaggac accgtctttg cctctcctcc ctgtcgtgga gcaggaagca tcgcgtaaaa
    10141 acgcatttgc aactgtcggc gcttttccag tatgttgcta aagattttat gaaaaacggc
    10201 ctgcgggccg ttttgttttg tctggatttt gcgctttttg cccagcattc agacgaaaat
    10261 tgcccgggaa ttgtgaaaaa atacgcgaca gcgcgcaata accgttctcg actcataaaa
    10321 gtgatgccgc tataatgccg cgtcctattt gaatgctttc gggatgattc tggtaacagg
    10381 gaatgtgatt gattataaga acatcccggt tccgcgaagc caacaacctg tgcttgcggg
    10441 gtaagagttg accgagcact gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa
    10501 ccactcaagg ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg
    10561 ctgttaaaag cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag
    10621 gcaaagtgcc aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc
    10681 tgggtgacct gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg
    10741 ctggcgcacc gacttatgtt ccgggcgaat acaagctggg tgaagacttc acttactctg
    10801 tagagtttga agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa
    10861 aaccgatcgt tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac
    10921 agcaggcgac ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg
    10981 acttcaccgg ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac
    11041 tggcgatggg ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag
    11101 ctggcgaaga gttcaccatc gacgtgacct tcccggaaga ataccacgca gaaaacctga
    11161 aaggtaaagc agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg
    11221 aactgactgc agaattcatc aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc
    11281 gcgctgaagt gcgtaaaaac atggagcgcg agctgaagag cgccatccgt aaccgcgtta
    11341 agtctcaggc gatcgaaggt ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga
    11401 ttgacagcga aatcgacgtt ctgcgtcgtc aggctgcaca gcgtttcggt ggcaacgaaa
    11461 aacaagctct ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg
    11521 ttggcctgct gctgggcgaa gttatccgca ccaacgagct gaaagctgac gaagagcgcg
    11581 tgaaaggcct gatcgaagag atggcttctg cgtacgaaga tccgaaagaa gttatcgagt
    11641 tctacagcaa aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg gaagaacagg
    11701 ctgttgaagc tgtactggcg aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc
    11761 tgatgaacca gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac aaaggcccgt
    11821 caccgccagg tggtgggctt ttttttgtca tgaattttgc atggaaccgt gcgaaaagcc
    11881 tctttcggtg ttagcgtaac aacaaaagat tgttatgctt gaaatatggt gatgccgtac
    11941 ccataacaca gggactagct gataatccgt ccataaggtt acaatcggta cagcaggttt
    12001 tttcaatttt atccaggaga cggaaatgtc atacagcggc gaacgagata actttgcacc
    12061 ccatatggcg ctggtgccga tggtcattga acagacctca cgcggtgagc gctcttttga
    12121 tatctattct cgtctactta aggaacgcgt catttttctg actggccagg ttgaagacca
    12181 catggctaac ctgattgtgg cgcagatgct gttcctggaa gcggaaaacc cagaaaaaga
    12241 tatctatctg tacattaact ccccaggcgg ggtgatcact gccgggatgt ctatctatga
    12301 caccatgcag tttatcaagc ctgatgtcag caccatctgt atgggccagg cggcctcgat
    12361 gggcgctttc ttgttgaccg caggggcaaa aggtaaacgt ttttgcctgc cgaattcgcg
    12421 cgtgatgatt caccaaccgt tgggcggcta ccagggccag gcgaccgata tcgaaattca
    12481 tgcccgtgaa attctgaaag ttaaagggcg catgaatgaa cttatggcgc ttcatacggg
    12541 tcaatcatta gaacagattg aacgtgatac cgagcgcgat cgcttccttt ccgcccctga
    12601 agcggtggaa tacggtctgg tcgattcgat tctgacccat cgtaattgat gccagaggcg
    12661 caactgtgcc gctatactta tccagggcgg cacaacgctg taagcggctt gcgcctgaga
    12721 atggcatttg cgtcgtcgtg tgcggcacaa agaacaaaga agaggttttg acccatgaca
    12781 gataaacgca aagatggctc aggcaaattg ctgtattgct ctttttgcgg caaaagccag
    12841 catgaagtgc gcaagctgat tgccggtcca tccgtgtata tctgcgacga atgtgttgat
    12901 ttatgtaacg acatcattcg cgaagagatt aaagaagttg caccgcatcg tgaacgcagt
    12961 gcgctaccga cgccgcatga aattcgcaac cacctggacg attacgttat cggccaggaa
    13021 caggcgaaaa aagtgctggc ggtcgcggta tacaaccatt acaaacgtct gcgcaacggc
    13081 gataccagca atggtgtcga gttgggcaaa agtaacattc tgctgatcgg tccgaccggt
    13141 tccggtaaaa cgctgctggc tgaaacgctg gcgcgcctgc tggacgtccc gttcaccatg
    13201 gccgacgcaa ccacgctgac cgaagccggt tatgtgggcg aagacgttga aaacatcatt
    13261 cagaagctgt tgcagaagtg cgattacgac gtacagaaag cgcagcgcgg gattgtctac
    13321 atcgatgaaa tcgacaagat ttctcgtaag tcagacaacc cgtccattac ccgagacgtt
    13381 tccggtgaag gcgtacagca ggcactgttg aaactgatcg aaggtacggt agctgctgtt
    13441 ccaccgcaag gtgggcgtaa acatccgcag caggaattct tgcaggttga tacctctaag
    13501 atcctgttta tttgtggcgg tgcgtttgcc ggtctggata aagtgatttc ccaccgtgta
    13561 gaaaccggct ccggcattgg ttttggcgcg acggtaaaag cgaagtccga caaagcaagc
    13621 gaaggcgagc tgctggcgca ggttgaaccg gaagatctga tcaagtttgg tcttatccct
    13681 gagtttattg gtcgtctgcc ggttgtcgca acgttgaatg aactgagcga agaagctctg
    13741 attcagatcc tcaaagagcc gaaaaacgcc ctgaccaagc agtatcaggc gctgtttaat
    13801 ctggaaggcg tggatctgga attccgtgac gaggcgctgg atgctatcgc taagaaagcg
    13861 atggcgcgta aaaccggtgc ccgtggcctg cgttccatcg tagaagccgc actgctcgat
    13921 accatgtacg atctgccgtc catggaagat gtcgaaaaag tggttatcga cgagtcggta
    13981 attgatggtc aaagcaaacc gttgctgatt tatggtaagc cggaagcgca acaggcatct
    14041 ggtgaataat taaccattcc catacaatta gttaaccaaa aaggggggat tttatctccc
    14101 ctttaatttt tcctctattc tcggcgttga atgtggggga aacatcccca tatactgacg
    14161 tacatgttaa tagatggcgt gaagcacagt cgtgtcatct gattacctgg cggaaattaa
    14221 actaagagag agctctatga atcctgagcg ttctgaacgc attgaaatcc ccgtattgcc
    14281 gctgcgcgat gtggtggttt atccgcacat ggtcatcccc ttatttgtcg ggcgggaaaa
    14341 atctatccgt tgtctggaag cggcgatgga ccatgataaa aaaattatgc tggtcgcgca
    14401 gaaagaagct tcaacggatg agccgggtgt aaacgatctt ttcaccgtcg ggaccgtggc
    14461 ctctatattg cagatgctga aactgcctga cggcaccgtc aaagtgctgg tcgaggggtt
    14521 acagcgcgcg cgtatttctg cgctctctga caatggcgaa cacttttctg cgaaggcgga
    14581 gtatctggag tcgccgacca ttgatgagcg ggaacaggaa gtgctggtgc gtactgcaat
    14641 cagccagttc gaaggctaca tcaagctgaa caaaaaaatc ccaccagaag tgctgacgtc
    14701 gctgaatagc atcgacgatc cggcgcgtct ggcggatacc attgctgcac atatgccgct
    14761 gaaactggct gacaaacagt ccgttctgga gatgtccgac gttaacgaac gtctggaata
    14821 tctgatggca atgatggaat cggaaatcga tctgctgcag gttgagaaac gcattcgcaa
    14881 ccgcgttaaa aagcagatgg agaaatccca gcgtgagtac tatctgaacg agcaaatgaa
    14941 agctattcag aaagaactcg gtgaaatgga cgacgcgccg gacgaaaacg aagccctgaa
    15001 gcgcaaaatc gacgcggcga agatgccgaa agaggcaaaa gagaaagcgg aagcagagtt
    15061 gcagaagctg aaaatgatgt ctccgatgtc ggcagaagcg accgtagtgc gtggttatat
    15121 cgactggatg gtacaggtac cgtggaatgc gcgcagcaag gtcaaaaaag acctgcgtca
    15181 ggcgcaggaa atccttgata ccgaccatta tggtctggag cgcgtgaaag atcgcatcct
    15241 tgagtacctt gcggttcaaa gccgtgtcaa caaaatcaag ggaccgatcc tctgcctggt
    15301 agggccgccg ggggtaggta aaacctctct tggtcagtcc attgccaaag ccaccgggcg
    15361 taaatatgtc cgtatggcgc tgggcggcgt gcgtgatgaa gcggaaatcc gtggtcaccg
    15421 ccgtacttac atcggttcta tgccgggtaa actgatccag aaaatggcga aagtgggcgt
    15481 gaaaaacccg ctgttcctgc tcgatgagat cgacaaaatg tcttctgaca tgcgaggcga
    15541 tccggcctct gcactgcttg aagtgctgga tccagagcag aacgtagcgt tcagcgacca
    15601 ctacctggaa gtggattacg atctcagcga cgtgatgttt gtcgcgacgt cgaactccat
    15661 gaacattccg gcaccgctgc tggatcgtat ggaagtgatt cgcctctccg gttataccga
    15721 agatgaaaaa ctgaacatcg ccaaacgtca cctgctgccg aagcagattg aacgtaatgc
    15781 actgaaaaaa ggtgagctga ccgtcgacga tagcgccatt atcggcatta ttcgttacta
    15841 cacccgtgag gcgggcgtgc gtggtctgga gcgtgaaatc tccaaactgt gccgcaaagc
    15901 ggttaagcag ttactgctcg ataagtcatt aaaacatatc gaaattaacg gcgataacct
    15961 gcatgactac ctcggtgttc agcgtttcga ctatggtcgc gctgataacg aaaaccgtgt
    16021 cggtcaggta accggtctgg cgtggacgga agtgggcggt gacttgctga ccattgaaac
    16081 cgcatgtgtt ccgggtaaag gcaaactgac ctataccggt tcgctcggcg aagtgatgca
    16141 ggagtctatt caggcggcgt taacggtggt tcgtgcgcgt gcggaaaaac tggggatcaa
    16201 ccctgatttt tacgaaaaac gtgacatcca cgtccacgta ccggaaggtg cgacgccgaa
    16261 agatggtccg agtgccggta ttgctatgtg caccgcgctg gtttcttgcc tgaccggtaa
    16321 cccggttcgt gccgatgtgg caatgaccgg tgagatcact ctgcgtggtc aggtactgcc
    16381 gatcggtggt ttgaaagaaa aactcctggc agcgcatcgc ggcgggatta aaacagtgct
    16441 aattccgttc gaaaataaac gcgatctgga agagattcct gacaacgtaa ttgccgatct
    16501 ggacattcat cctgtgaagc gcattgagga agttctgact ctggcgctgc aaaatgaacc
    16561 gtctggcatg caggttgtga ctgcaaaata gtgacctcgc gcaaaatgca ctaataaaaa
    16621 cagggctggc aggctaattc gggcttgcca gccttttttt gtctcgctaa gttagatggc
    16681 ggatcgggct tgcccttatt aaggggtgtt gtaaggggat ggctggcctg atataactgc
    16741 tgcgcgttcg taccttgaag gattcaagtg cgatataaat tataaagagg aagagaagag
    16801 tgaataaatc tcaattgatc gacaagattg ctgcaggggc tgatatctct aaagctgcgg
    16861 ctggccgtgc gttagatgct attattgctt ccgtaactga atctctgaaa gaaggggatg
    16921 atgtagcact ggtaggtttt ggtacttttg ccgttaaaga gcgtgctgcc cgtactggcc
    16981 gcaacccgca gaccggtaaa gagatcacca tcgctgctgc taaagtaccg agcttccgtg
    17041 caggtaaagc actgaaagac gcggtaaact aagcgttgtc cccagtgggg atgtgacgaa
    17101 gttcaagggc gcatctactg atgtgccttt tttatttgta ttcggtgact ttctgtgtct
    17161 tgtgggctga caattgcccc cgtttcttgt cacaataggc ctttgcgcgc atcgatacgt
    17221 tgcgtgaggt acacagtcat ctacagcgga gtgttgttac accatgatgg acagcttacg
    17281 cacggctgca aacagtctcg tgctcaagat tattttcggt atcattatcg tgtcgttcat
    17341 attgaccggc gtgagtggtt acctgattgg cggaggcaat aactacgccg caaaagtgaa
    17401 tgaccaggaa atcagccgtg ggcagttcga gaacgccttt aacagcgagc gtaatcgcat
    17461 gcagcaacag ctgggcgatc aatactccga gctggcagcg aacgaaggct atatgaaaac
    17521 cctgcgtcaa caggtgctga atcgtctgat cgacgaggcg ctgttggatc agtacgctcg
    17581 tgagctgaaa ctgggtatca gtgatgagca ggttaaacag gcgattttcg cgaccccagc
    17641 cttccaggtt gacggcaaat ttgataacag ccgctataac ggtatcctca accagatggg
    17701 gatgaccgcc gatcagtacg cccaggcgct gcgtaaccag ctcactaccc aacagctgat
    17761 taacggtgtt gccggtaccg attttatgct gaaaggtgaa accgacgagc tggcggcact
    17821 ggtcgcgcaa caacgcgtgg tgcgtgaggc gactatcgat gttaacgcgc tggcggcgaa
    17881 gcaacctgtg accgaacagg agattgccag ctactacgaa caaaacaaaa acaatttcat
    17941 gacgccggaa caattccgcg tgagttacat caagctggat gccgcaacga tgcagcaacc
    18001 ggttagcgat gcggatatcc agagctacta cgatcagcat caggatcaat tcactcagcc
    18061 gcagcgtacc cgctacagca tcatccagac caaaactgaa gatgaagcga aagcggtact
    18121 tgatgagctg aataaaggcg gtgattttgc tgcattagcc aaagaaaaat ctgccgatat
    18181 tatctctgcc cgtaacggcg gcgatatggg ttggttagaa gatgccacta tccccgacga
    18241 actgaaaaat gctggtctga aagaaaaagg gcaactgtct ggtgtcatca aatcttcggt
    18301 cggtttcctg attgtacgtc tggacgacat tcagccagcg aaagtgaaat cgttagacga
    18361 agtacgtgac gacattgcgg cgaaagtgaa acacgaaaaa gccctcgatg cgtactatgc
    18421 gctgcagcag aaagtgagcg atgcggcaag caacgacacc gagtctctgg ccggtgcaga
    18481 gcaagctgcc ggcgttaaag ccactcagac gggttggttc agcaaagata acctgccgga
    18541 agagttgaac ttcaagccgg ttgccgacgc tatctttaac ggcggtctgg taggtgaaaa
    18601 cggcgcgccg ggcatcaact ctgacatcat caccgtagac ggcgaccgcg cattcgtgct
    18661 gcgcatcagc gagcacaaac cggaagcggt gaaaccgttg gcagatgttc aggaacaagt
    18721 taaggcattg gttcagcaca acaaagctga acaacaggcg aaagtggatg ctgagaaact
    18781 gctggttgat ttgaaagccg gcaaaggtgc ggaagctatg caggctgcca gtctgaaatt
    18841 tggcgagccg aaaaccttaa gccgttccgg tcgtgacccg attagccagg cggcgtttgc
    18901 actgccactg ccagcgaaag acaaaccgag ctacggtatg gcgaccgata tgcaaggtaa
    18961 tgtggttctg ctggcgctgg atgaagtgaa acaaggttca atgccggaag atcagaaaaa
    19021 agcgatggtg cagggtatca cccagaacaa cgcacaaatc gtctttgaag ctctgatgag
    19081 taacctgcgt aaagaggcga aaatcaaaat tggcgatgcg ctggaacagc aataatcctg
    19141 aagccgcctc gcaaaaaaat gcgttgcagc tgtaacaaga aaaggtcgct ttcgcggcct
    19201 tttccatttc tgaacattgc catttgttta ctgttttcac tgccgttaag gtgattccac
    19261 tgttaacaaa caaggagaaa acagtatgaa acacggaatt aaagcactgc tcattaccct
    19321 gtccctggcc tgtgccggaa tgtctcatag cgcgctggcg gcagcttctg tggcgaaacc
    19381 gacaacggta gaaaccaaag cggaagctcc tgcagcacaa agtaaagcag cagtaccggc
    19441 gaaagccagt gacgaagaag gcacccgggt cagcattaat aatgccagcg cggaagagct
    19501 agcccgcgcg atgaatggcg ttggcctgaa gaaagcgcag gcgattgtca gttatcgcga
    19561 agagtacggt ccgtttaaaa cggtggagga tctaaagcag gtgccgggga tgggcaattc
    19621 gctggtggaa cgtaatctgg cggtattaac cctgtaatta atttgcatag tggcaatttt
    19681 ttgccagact gaagaggtca taccagttat gacctctgta cttataacaa caacgtaagg
    19741 ttattgcgct atgcaaacac aaatcaaagt tcgtggatat catctcgacg tttaccagca
    19801 cgtcaacaac gcccgctacc ttgaatttct cgaagaagcc cgctgggatg ggttggaaaa
    19861 tagcgacagt tttcagtgga tgacggccca taacatcgcc ttcgtcgtgg tcaatatcaa
    19921 tattaactat cgtcgcccag cggtattaag tgacctgtta actattacca gtcagttgca
    19981 gcaattaaac ggtaaaagcg gcatcttaag ccaggtcatt acactggagc cggaagggca
    20041 ggtggtagcg gatgcgctta ttacgtttgt ttgtattgat cttaaaacgc agaaagcatt
    20101 agctctggaa ggggaattgc gcgaaaagct ggagcagatg gttaagtaaa cgttttgtgg
    20161 tgccggatgc tcaagccgca tccggcgaca cccggaataa ttacctcaac ccggttttct
    20221 gcttcatcgc tgccatcacc gtcggtttat cggccagata atgattcaaa ccgttggcgc
    20281 gtaaattaca tgccgcacaa tgaccgcaac cgtcgccttt aatgccgtta tagcaggtca
    20341 acgtttcgtt acggactaaa tccagtttgc cgtaataatc tgccagcgcc caggtttccg
    20401 ctttatcaat ccacatcagc ggcgtttcaa aacgaatatc tttcgccatg cccaaactga
    20461 cggcatggtt tagtgctttc acaaactcat cgcggcaatc cgggtagccg gagaaatccg
    20521 tttcacagac gccggtaatt acggcttctg cttttacctg atacgcatat attgccgcca
    20581 gcgtcaggaa caaaatatta cgccctggga caaacgtatt cgggatgcca tcggcttcag
    20641 gttcataatc aggcaccgga atgctgtcac gtgtcaggct gctgaccgcc agctcgttga
    20701 gcagcgttac atcaagaacc ttatgcgcgc gtgctcccag tttcagcgcc agttcgcgtg
    20761 ccacgtcgat ttctgcgcga tgtcgctgac cgtaatcgaa cgtcacgcaa tggacttcat
    20821 catattgttg taatgcctgc accagacagg tggtggaatc ctggcctcca ctgaacacaa
    20881 cgacagcacg tttcatagat aatcctgctt gaacaataaa agcgttatgg taacgcctgc
    20941 gattaacccg gaccagctat tcattgcgac ggcgcgggaa gccaggcttc ggtaaattca
    21001 aaccagccgc gcggtgtcag tcgcacaccg ttcacgccgg gaggggcact aatgcgatag
    21061 tgatagttga acagcggcgt cagcgtcgcg tctgccatta actggctaaa aaccgctttc
    21121 agggcattaa atcggttttc ctcatcaggc attacttgca ccgcgtccag cgtcgattgc
    21181 agatgtgcat atgctggagc gtcgaaaaca tgtggccata gtggatcgca acgtagccat
    21241 tgctccagag tatattccgg tgcttcgcca attaatctgt cgcccatcat gaggtctgcg
    21301 tgtgccaata gggtcgtgtc gtcccagttt tttgcgttat gaaaaataat tgtaagttca
    21361 cagccttccg ctgccagtgt cgcctgtagg cgttctgcca tggtatgaag ttctatcggt
    21421 aggtgataaa ccagcgtcaa ggttttcggt agtttgactt catccggtac ttgccattgc
    21481 ggaatagtcc agcctggcag taatgcatga ctggcggtga tcaggttttc tcctacttct
    21541 aacgtttgta ataaaccgga ttgatgaata atggagatca cttttcgcgc ctgccagagg
    21601 gagagtcggg gacttttgcg caacgttaaa tagcaaaaac ctaaactgat gccactactg
    21661 acctggctga cccgttgcag ctcctccggt ttgccgatgg tgatttgcac gggatgccga
    21721 cagctggttc ccaaatcttt ttcgaaaagc ggcggagtta tccagtactc aaccgcttta
    21781 agcagtggat gacgtaaatg gtaataatca tggctttcca ggcgcaccag ttccgctgtg
    21841 aattgtgtta agcgaaaagg acccgtgccg atcagtggga attgcggatg cgccagatgg
    21901 ctacaatagc tcgccagccg gtgcgcaagc cagtaatcag ggcgatgtaa aaagaaggtc
    21961 agacactgcg gatgggtgac ttcaatacgc ttcacgctaa taaataattg atccagtgct
    22021 ggcagttgta acagcatcaa taatcgctgg tgtaagtgtg aggcttttac tgcatcgccg
    22081 ttatgccagt gtagggttga acgaagataa aagtcccagc gtaacccgtc agtagaggtt
    22141 tcccagtgat gcgctaaatc gccaatcggg cgctgggtat tattatcgaa gcgggtcagg
    22201 ccggaaaata tctgtccggc gagatgctgc tcggcacggc cgggcaaaaa gcctggttgt
    22261 agcggttcga gcgggcgata gtagggaata cgcaacgtgg gtgtatcgtt ttgccattgt
    22321 ccgcccataa acggctgtaa cagagtgcgc agctcacctg gggccagttg cgccagctcc
    22381 agcacatctt gctgctttcc ggtttccagt gcctgttcca tcatcgcatt gcgtagcgat
    22441 tccggcgtga ccagaaagcg taattgtccg cgctttccgc gtcctgactg cgcctgccac
    22501 tccagccatc ccgcctcctg tgcctgacgc aacagcgtac gaacatggcg ttcgctgcaa
    22561 aaacagcgtt cggccagttc attgacggtg acagcttgcg gctttccggc ggaaggttgc
    22621 cacagacgtt gatactggtt aagacggttg agcaatcgca tataaacccg gaacaatatt
    22681 atttaactat tcactattac ttccgtatat atcaggtgat actcaatcac cattaaccgt
    22741 gtcacagagt ggagaaagaa atggctcgtc tggcagcatt tgatatggat ggcactttat
    22801 tgatgcctga ccatcattta ggtgagaaaa ccctctctac tttggcgcga ctgcgtgaac
    22861 gcgacattac cctcactttt gccacggggc gtcatgcgct ggagatgcag catattctcg
    22921 gggcgctatc gctggatgca tatttgatta ccggcaacgg aacgcgcgtg cattctctgg
    22981 aaggtgaact tttacattgt gatgatttac ctgcggatgt cgcggagctg gtgctgtatc
    23041 agcaatggga tacccgagcc agcatgcata tcttcaatga cgacggttgg tttaccggga
    23101 aagagatccc tgcgttgttg caggcatttg tctatagcgg ttttcgttat cagataatcg
    23161 atgtcaaaaa aatgccactc ggcagcgtca ccaagatctg cttctgtggc gatcacgacg
    23221 atcttacacg cttgcagatc cagctatacg aagcattagg cgagcgtgca catttgtgtt
    23281 tttccgccac ggattgcctc gaagtgctgc cggtgggctg caataaaggc gctgcattga
    23341 cggtgctgac ccaacattta ggtttatcgt tgcgcgattg catggccttt ggtgatgcga
    23401 tgaacgatcg cgaaatgtta ggcagcgtcg gtagcggctt tattatgggc aatgcgatgc
    23461 cgcaactgcg cgcggagctc ccgcatttac cggtgattgg acattgccga aatcaggctg
    23521 tctctcacta tttgacgcac tggctggact atccacatct accttattcc cccgaataac
    23581 gagatccctt ccagcaccgg gcaattgccc ggtttttttt gcgttgaatt tgtcattttg
    23641 tgccgtggtg tttaaaccgc acagaataaa ttgtcgcgat ttcaccttta aaatagaatt
    23701 aaaagagaaa aaaattctcc gtggaagggc tatgttagat aaaattgacc gtaagctgct
    23761 ggccttactg cagcaggatt gcaccctctc tttgcaggca ctggctgaag ccgttaatct
    23821 gacaaccacc ccttgctgga agcgcctgaa acggctggag gacgacggta tccttatcgg
    23881 caaagtcgcc ctgctggatc cggaaaaaat aggcctcggc ctgaccgcct ttgtgctgat
    23941 aaaaacgcaa catcacagca gcgaatggta ttgccgcttt gtcacggtgg ttaccgaaat
    24001 gccagaagtg ctggggttct ggcgcatggc tggtgaatac gattatctga tgcgcgtcca
    24061 ggttgccgac atgaaacgct acgacgagtt ttataagcgt ctggtaaaca gcgtgccggg
    24121 gctgtcggac gtcacttcca gcttcgcgat ggaacagatt aaatacacca cttctttacc
    24181 catcgaataa atatccagaa tcaggtcagg acacaacgcg tgcgattatt tgctcaatta
    24241 agctggtatt tccgtcggga atggcgtcgc tatctcgggg ctgtcgcctt gcttgtcatt
    24301 atcgcgatgc tgcaactggt tccgccaaaa gtggttggta ttgttgtcga tggcgtgaca
    24361 gaacaacact ttactaccgg gcagatcctg atgtggatcg ccaccatggt gctgattgcc
    24421 gttgtggttt atctcctgcg ttacgtctgg cgggtattgc tgtttggtgc gtcttatcaa
    24481 ctggctgttg aactgcgtga agattattac cgtcagctaa gccggcagca tcctgagttt
    24541 tacctgcgtc atcgcacggg tgacctcatg gctcgtgcga ctaatgacgt cgatcgcgtc
    24601 gtgtttgccg ccggagaagg ggtgctgacg ctggtggatt cactggtaat gggctgcgct
    24661 gtgttgatta tgatgtctac gcaaattagc tggcagttga ccttatttgc cctgttaccg
    24721 atgccagtga tggcgatcat gattaagcgc aacggcgatg ccttgcatga acgctttaag
    24781 ctggcacagg cggcgttttc tagtcttaat gaccgcaccc aggaaagcct caccagtatt
    24841 cgcatgatca aagcctttgg tctggaagat cgccagtcgg cgttatttgc cgcggatgcc
    24901 gaagataccg gcaaaaaaaa catgcgggtg gcgcgtattg atgctcgttt cgacccgacc
    24961 atctatatcg cgattggtat ggcgaacttg ctggcgattg gcggcggtag ttggatggtg
    25021 gtgcagggca gtttaacgct gggccagctc accagtttta tgatgtattt aggtctgatg
    25081 atttggccaa tgctggcgct ggcatggatg tttaacattg tggaacgtgg tagtgctgcg
    25141 tacagccgta ttcgcgcgat gctggcggaa gcgccggtgg tgaacgatgg tagcgaaccc
    25201 gtgccggaag ggcgtggcga actggatgta aatattcacc agttcacgta tccgcagact
    25261 gaccatcctg cgctggaaaa cgtcaatttc gccctgaaac ccggtcagat gctgggtatt
    25321 tgcgggccga ctggttccgg caaaagtacc ctgttgtcgc tcattcagcg tcatttcgac
    25381 gtcagcgagg gggatattcg ctttcatgat attcctctga cgaagttaca actcgatagc
    25441 tggcgtagcc gcctggcggt ggtcagccag acgccattcc ttttttctga caccgtggcg
    25501 aataacatcg cgctgggttg cccgaatgcc acccagcaag agattgaaca cgtagcgcgg
    25561 ttagccagcg tacatgacga tattttgcgt ctgccgcaag gttacgatac agaagtgggc
    25621 gagcgcggcg tgatgctttc cggtgggcaa aaacagcgta tctccattgc tcgagcgtta
    25681 ttagtcaacg cggaaatcct catccttgat gatgcgcttt cggcggtgga cggacgcaca
    25741 gagcaccaga tcctgcataa cctgcgtcag tggggacagg gaagaacggt aatcatcagt
    25801 gcccatcgcc tttcggcact gacggaagcc agtgaaatta ttgtgatgca gcacggacat
    25861 atcgcccagc gtggcaatca tgatgagctg gcgcaacaaa gcggctggta tcgcgatatg
    25921 tatcgctatc aacaactgga ggcggcgctc gacgacgttc cggaaattcg cgaggaggcc
    25981 gtcgatgcgt agttttagcc aactgtggcc gactctcaag cgtctgttag cgtacggttc
    26041 gccgtggcgg aaaccgctgg ggattgcggt cctgatgatg tgggttgcgg cagcggcaga
    26101 agtcagtggg ccgctgctta tcagctattt tatcgacaat atggtagcga aaaataacct
    26161 gccgttgaaa atggttgcag ggctggcggc ggcgtatgtt gggctacaac tgtttgccgc
    26221 cgggctacat tacgcgcagt cgctgctgtt taatcgggcg gcagtaggcg tagtgcaaca
    26281 gttgcgtacc gacgtgatgg atgctgcgtt acgccagcca ttaagcgagt ttgataccca
    26341 acccgtcggg caggtgattt cccgcgtcac taatgacact gaagtgatcc gcgatctcta
    26401 cgttaccgta gtggcaactg tcctgcgtag cgccgcactg gtgggcgcga tgctggtggc
    26461 aatgttcagc ctcgactggc gaatggcgct ggtggcgata atgattttcc cggtagtgct
    26521 ggtggtaatg gtgatatatc aacgttacag cacgccgatt gtccgtcgtg tgcgcgccta
    26581 tctggcggat atcaacgacg gctttaacga aatcatcaat ggcatgagcg ttatccagca
    26641 gttccgtcag caggcgcgat ttggcgaacg tatgggtgag gccagtcgtt cacactatat
    26701 ggcgaggatg caaaccctgc gcctcgacgg ttttctgctg cgtccgctgc tgagtctgtt
    26761 ttcatcgctc attctttgtg gcttgttgat gctgtttggc ttctccgcca gcggcaccat
    26821 tgaagtgggc gtgttatatg cgtttatcag ctatcttggg cgacttaacg aacccttaat
    26881 cgaactgacc acgcagcagg cgatgctgca acaggctgtt gttgctggtg agcgcgtgtt
    26941 tgaactgatg gacggaccgc gccagcaata tggcaatgat gatcgcccgt tacagagcgg
    27001 caccatcgaa gtcgataacg tgtcatttgc ttatcgcgat gacaatctgg tgctaaagaa
    27061 cattaatctc tctgtgcctt cgcgtaactt tgtggcgctg gtcgggcata ccggcagtgg
    27121 caaaagcacc ctcgccagtt tattgatggg ctattacccg ctaacggaag gtgagattcg
    27181 ccttgatggt cgtccattaa gttcgctaag tcacagcgcg ctgcgccagg gcgtggcaat
    27241 ggtgcagcaa gatccggtgg tgctggcgga taccttcctc gccaacgtga cgctgggacg
    27301 tgatatctcc gaagaacgcg tctggcaggc gctggaaacc gtgcaactgg cggagctggc
    27361 gcgcagcatg agtgatggta tttatacgcc gctgggcgag cagggaaata atctctcagt
    27421 cgggcaaaag caactgctgg cactggcgcg cgtgctggtg gagacgccgc aaatcctgat
    27481 ccttgatgag gcaaccgcca gcattgactc cggtactgaa caggcgattc aacatgctct
    27541 ggcggcggtg cgtgaacata ccacgctggt ggtgattgct caccgcttat caactattgt
    27601 tgatgccgac accattctgg tgcttcatcg tgggcaagcc gtggagcagg gcactcacca
    27661 gcaactgctg gcggcccagg gacgctactt gcagatgtat caactgcaac ttgcgggcga
    27721 agagctggca gccagcgtgc gtgaagagga atcattgagc gcctgaatag cgcaatattt
    27781 catcgttggt gcaaaaatgt aacgcactgt gcactgtcat agtgcgtttt cattttcaat
    27841 cttcttaact tcctgctctc tttctcgttt ttcatttctg gcacaccgct tgcaatacct
    27901 tcttcgtgta gcagaaccat taccgaattc tgaccggagg ggatctatga agctggtgac
    27961 cgtgataatc aaaccattca agctggaaga cgttcgtgaa gcgttatctt ccattggtat
    28021 tcagggcctg accgtcaccg aagtgaaagg tttcgggcgt cagaaagggc atgccgagct
    28081 gtaccggggg gcggaataca gcgtcaactt cctgccaaaa gtaaaaattg atgtggcgat
    28141 tgctgatgac caactcgatg aagtgatcga tatcgtcagt aaggcggctt acaccggaaa
    28201 aattggcgac ggcaaaatct tcgtcgctga attgcaacgc gtcattcgta ttcgtaccgg
    28261 cgaagccgac gaagcggcgc tgtaatctct ggcacacagc aacaggaacg aaaaatgaag
    28321 atagcgacga taaaaactgg gcttgcttca ctggcgatgc tgccgggact ggtaatggct
    28381 gcacctgcgg tggccgataa agccgacaat gcgtttatga tgatttgtac tgcgctggtg
    28441 ctgtttatga ctattccggg gattgccctg ttttacggtg ggttgattcg cggcaaaaac
    28501 gtgctgtcga tgctgacgca ggtgacggtg acgtttgcac tggtctgtat tctctgggtg
    28561 gtttacggtt actcgctggc gtttggtgag ggcaacaact tcttcggcaa cattaactgg
    28621 ttgatgctaa aaaacatcga actgacggcg gtgatgggca gcatttatca gtatatccac
    28681 gtggcgtttc agggctcgtt tgcctgcatt accgtcggct tgatcgttgg ggcgctggcg
    28741 gaacgaatcc gcttctcagc tgtgttgatt ttcgtggtgg tatggctgac gctctcttac
    28801 attccgattg cgcatatggt gtggggcggt ggtttgctgg cttctcacgg tgcgctggat
    28861 ttcgcgggtg gcacggtggt gcatattaac gccgcaatcg ccggtctggt gggcgcgtat
    28921 ctgataggca aacgcgtggg cttcggcaaa gaggcgttta aaccgcacaa cctgccaatg
    28981 gtcttcaccg gtactgccat tctctatatt ggttggtttg gctttaacgc cgggtcagcg
    29041 ggcacggcga atgaaatcgc ggcactggca tttgtgaata ctgtggtcgc aacggcggcg
    29101 gcaattcttg gctggatctt cggtgaatgg gcgctgcgtg gtaagccttc tctgctggga
    29161 gcgtgttctg gcgcgattgc cggtctggtc ggcgtgacgc cagcgtgtgg ctacattggg
    29221 gttggcggcg cgttgattat cggcgtggta gctggtctgg cgggcttgtg gggcgttact
    29281 atgctcaaac gcttgctgcg ggtggatgat ccctgcgatg tcttcggtgt gcacggcgtt
    29341 tgtggcattg tcggctgtat catgactggg atttttgccg ccagttcgct gggtggcgtg
    29401 ggcttcgctg aaggtgtgac gatgggtcat cagttgctgg tacagctgga aagcatcgcc
    29461 attacgatcg tctggtccgg tgtggtggca tttattggct acaaactggc ggatctgacg
    29521 gttggtctgc gtgtaccgga agagcaggag cgagaagggc tggatgtcaa cagtcacggc
    29581 gagaatgcct ataacgcgta acaagcactg caaaaaacag ccggacggtt ttcacctccg
    29641 gctatttttt taattgtgat tacgcatcac cccttcctga acggtcgagg caaccagtac
    29701 gccgtcctgg gtataaaact caccgcgcac aaagccacgt gcgctggacg ccgaggtgct
    29761 ctccacgcta tacagcagcc attcattcaa attaaacggg cgatggaacc acatggaatg
    29821 gtcaatggtg gcaatctgaa tccccggttc gagaaaaccg atgccgtgcg gctgtagagc
    29881 taccggcagg aagttaagat cagaagcgta accgagcaga tactgatgaa cgcgcaggtc
    29941 atccggcacg ctaccatttg cgcggatcca cacctgacga tgtggttctg cgacgtgacc
    30001 tttcagtggg ttatgaaact ccaccggacg gacttccagc ggacgatcgc agatgaattt
    30061 atctttaagc actggcggca gcaggtgcgc cagcgattgg gcgatttgcg tttccgaagg
    30121 caggccatca ggcgctggcg cggacggcat tgttttttga tgttcgaaac ccgcttctgg
    30181 tgcctggaaa gaggccgtca tataaaaaat cggtttgccg ttttgaatag cagcaacccg
    30241 gcgggcgctg aagctgttac cgtcacgcag cgtttcgaca tcataaataa tcggcttctt
    30301 actatcgcca gggcgaagaa agtagctgtg aaacgaatgt accagccgct cttcagggac
    30361 ggtctctttt gcagcataca aggcctgacc cacgacctgg ccgccaaaca cctggcgtaa
    30421 acctaaatct tcactctggc cgcgaaagag tccttcctca attttttcca gatttaacaa
    30481 tgtcagtaaa ttttttagcg cctgactcat ataactctcc agtaacaaag ctgccgcagc
    30541 aagccaaagt gagttgagta taacgcaaat ttgctactgg tccgatgggt gcaatggtct
    30601 gaattacggg ctaattacag gcagaaatgc gtgatgtgtg ccacacttgt tgacgttact
    30661 attttgttaa ccactcttcc ggcgaggaaa gttagcccgc tggtgcattg ataataagga
    30721 gaaatgaatg aaactcgtgc acatggccag tggtttagcg gttgcgattg cgttggcggc
    30781 ttgcgcagat aaaagcgcgg atattcagac gccagccccg gctgcaaata cgtctatttc
    30841 agcaacacaa caacctgcta tccagcaacc gaatgtctcc ggtaccgtct ggatccggca
    30901 gaaagtcgca ctgccgcctg atgctgtgct gaccgtgaca ctttctgacg cgtcgttagc
    30961 cgatgcaccg tcaaaagtgc tggcgcagaa agcggtgcgt accgaaggta aacagtcacc
    31021 attcagcttt gttctgccat ttaatccggc agatgttcag ccgaacgcgc gtattctgtt
    31081 gagtgcggcg attaccgtga atgacaaact ggtatttatc accgataccg ttcagccggt
    31141 gatcaaccag ggcggaacta aagccgacct gacattggtg ccggtacagc aaaccgccgt
    31201 gccggttcag gccagcggtg gtgcaacgac taccgtacct tcgacttcac caactcaggt
    31261 gaatccgtct tcggcagttc cagctcctac gcaatattaa gcgacgttaa ccctctccgc
    31321 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
    31381 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggt ccggttagcg
    31441 aaattgtgcc gtggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
    31501 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
    31561 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
    31621 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
    31681 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgcg atgggttgca
    31741 attagccggg gcagcagtga taatgcgcct gcgcgttggt tctcaacgct ctcaatgggg
    31801 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
    31861 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
    31921 cccaccgttt cgtcaaaaaa ccccaacatg gctaaacttt aaccacaact gacgtcgcaa
    31981 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
    32041 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggctgc
    32101 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
    32161 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
    32221 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gctgctactc aaaagatgat
    32281 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
    32341 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
    32401 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
    32461 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
    32521 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
    32581 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
    32641 cagaatgtcg acatccagat cctgtaaata acttaaactt gaatatcccg taccaaaatc
    32701 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
    32761 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
    32821 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
    32881 aagattgatc gaaatatgct gctgtggatg ctgacgcagc cagtcgccca tatcttcaaa
    32941 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
    33001 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
    33061 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
    33121 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
    33181 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
    33241 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
    33301 catctccggg aagggcagga tatcgtaaat gattccatta ttttcgatat gctcaccggg
    33361 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
    33421 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
    33481 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
    33541 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
    33601 tttacctggc tcggggaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
    33661 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
    33721 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcttgcag
    33781 cgcatctttc ccttgtgtcg ccaccttatt ggcccgaata gcgacgcggg aggaataggt
    33841 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
    33901 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
    33961 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
    34021 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
    34081 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
    34141 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
    34201 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
    34261 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
    34321 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
    34381 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
    34441 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
    34501 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
    34561 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
    34621 gcaactttcc agggaacacc ctattttaac gtgggaatag tagagaggaa gtaacagatt
    34681 aaccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
    34741 ggattaccgc ccacgacaac gttgtccggg acatcttttg tgacaactgc acctgaggct
    34801 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
    34861 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
    34921 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
    34981 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattaccg
    35041 agaaaaatgt tatagccata gtcacagcga aacgttggct caatataagc ctctgtcacc
    35101 tgaccgaata gatcagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
    35161 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
    35221 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
    35281 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttcccattac
    35341 atctgtgtga taaagatcac atagggtatg tcttcatggt gaaaagtata aaattcttaa
    35401 taaacagccg
//
LOCUS       DAZFFI010000041        34694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFFI010000041 DAZFFI010000000
VERSION     DAZFFI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.041"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(183..338)
                     /locus_tag="ACHJ12_002321"
     CDS             complement(183..338)
                     /locus_tag="ACHJ12_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4990097.1"
                     /translation="MKQQKAMLIALIVICLTVIMTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(538..963)
                     /locus_tag="ACHJ12_002322"
     CDS             complement(538..963)
                     /locus_tag="ACHJ12_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4990098.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            complement(1042..1905)
                     /locus_tag="ACHJ12_002323"
     CDS             complement(1042..1905)
                     /locus_tag="ACHJ12_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4990099.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKINPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            complement(1905..2873)
                     /locus_tag="ACHJ12_002324"
     CDS             complement(1905..2873)
                     /locus_tag="ACHJ12_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO4990100.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            complement(2875..4533)
                     /locus_tag="ACHJ12_002325"
     CDS             complement(2875..4533)
                     /locus_tag="ACHJ12_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO4990101.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            complement(4567..4935)
                     /locus_tag="ACHJ12_002326"
     CDS             complement(4567..4935)
                     /locus_tag="ACHJ12_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990102.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRKGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            complement(5178..5471)
                     /locus_tag="ACHJ12_002327"
     CDS             complement(5178..5471)
                     /locus_tag="ACHJ12_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990103.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            complement(5490..5699)
                     /locus_tag="ACHJ12_002328"
     CDS             complement(5490..5699)
                     /locus_tag="ACHJ12_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990104.1"
                     /translation="MTLYEILKAEFKTNAAIGRRFPKRGKPRGSQGVGKWKTRGVPED
                     VAILCHLDPKIPYQHPSLMNASHES"
     gene            5810..6466
                     /locus_tag="ACHJ12_002329"
     CDS             5810..6466
                     /locus_tag="ACHJ12_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4990105.1"
                     /translation="MRTLGERLSSARQRAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVATALNCTPQWLQNGEEQSVNWENNVKSCPQKDAFHSYPVINWVQAGLFST
                     AGDDYSMYDHDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKAVHS
                     GCYVIARKKSTNEATFKKYVTEMGRSFLKPLNPQYPIIEIDDDCEIVGVIVDARWDIF
                     "
     gene            6552..6962
                     /locus_tag="ACHJ12_002330"
     CDS             6552..6962
                     /locus_tag="ACHJ12_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4990106.1"
                     /translation="MFQKTTLSCIEDLKNLGSLIALIAKAAPDTTLANDIESCAGMAW
                     DIINKVSHTLSLLIHKQNISDCPHEIRTQRESCGLSTAELATLLGVDEETIIQWENGK
                     AEPLASQVIPLANILDCDPMWLLTGFHSDTTKES"
     gene            6964..7155
                     /locus_tag="ACHJ12_002331"
     CDS             6964..7155
                     /locus_tag="ACHJ12_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990107.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            7152..7352
                     /locus_tag="ACHJ12_002332"
     CDS             7152..7352
                     /locus_tag="ACHJ12_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990108.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKRN"
     gene            7527..7889
                     /locus_tag="ACHJ12_002333"
     CDS             7527..7889
                     /locus_tag="ACHJ12_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990109.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            8297..8857
                     /locus_tag="ACHJ12_002334"
     CDS             8297..8857
                     /locus_tag="ACHJ12_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990110.1"
                     /translation="MKEEPEIIEINSEEFAENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHLLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFNKRANHDSN"
     gene            8844..9428
                     /locus_tag="ACHJ12_002335"
     CDS             8844..9428
                     /locus_tag="ACHJ12_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990111.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            9452..10321
                     /locus_tag="ACHJ12_002336"
     CDS             9452..10321
                     /locus_tag="ACHJ12_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO4990112.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGTTPIRIQQ
                     NHHENKSRFIEGKHFFKVVGDELKNLRLVLNESQNEMKISPKTRSLILWTERGAANHA
                     KLLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMVLENKTL
                     SATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTA
                     SYARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFK
                     HDKAIYTPEAEHE"
     gene            10314..10676
                     /locus_tag="ACHJ12_002337"
     CDS             10314..10676
                     /locus_tag="ACHJ12_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO4990113.1"
                     /translation="MNNTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYEDLPYEEAQNEYTGETNIMATIQVGDLACTDEGMLGEAWLA
                     DMLIAAEIIRIEPAGTLS"
     gene            10693..10917
                     /locus_tag="ACHJ12_002338"
     CDS             10693..10917
                     /locus_tag="ACHJ12_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990114.1"
                     /translation="MIISFTDYGEQAKLVITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            10914..11816
                     /locus_tag="ACHJ12_002339"
     CDS             10914..11816
                     /locus_tag="ACHJ12_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990115.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLDWVWAQLMEAAKFAYEGSVNG"
     gene            11809..12051
                     /locus_tag="ACHJ12_002340"
     CDS             11809..12051
                     /locus_tag="ACHJ12_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990116.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKARAPAQPDELA"
     gene            12072..12218
                     /locus_tag="ACHJ12_002341"
     CDS             12072..12218
                     /locus_tag="ACHJ12_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990117.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDMWCHCRG
                     LKIV"
     gene            12226..12480
                     /gene="xisR"
                     /locus_tag="ACHJ12_002342"
     CDS             12226..12480
                     /gene="xisR"
                     /locus_tag="ACHJ12_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4990118.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            12514..13803
                     /locus_tag="ACHJ12_002343"
     CDS             12514..13803
                     /locus_tag="ACHJ12_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4990119.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            13848..14498
                     /gene="rcsB"
                     /locus_tag="ACHJ12_002344"
     CDS             13848..14498
                     /gene="rcsB"
                     /locus_tag="ACHJ12_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4990120.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(14698..17547)
                     /gene="rcsC"
                     /locus_tag="ACHJ12_002345"
     CDS             complement(14698..17547)
                     /gene="rcsC"
                     /locus_tag="ACHJ12_002345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4990121.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(17822..18598)
                     /gene="yfaP"
                     /locus_tag="ACHJ12_002346"
     CDS             complement(17822..18598)
                     /gene="yfaP"
                     /locus_tag="ACHJ12_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4990122.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(18603..20252)
                     /gene="yfaQ"
                     /locus_tag="ACHJ12_002347"
     CDS             complement(18603..20252)
                     /gene="yfaQ"
                     /locus_tag="ACHJ12_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO4990123.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(20253..24767)
                     /locus_tag="ACHJ12_002348"
     CDS             complement(20253..24767)
                     /locus_tag="ACHJ12_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO4990124.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(24791..25414)
                     /gene="yfaT"
                     /locus_tag="ACHJ12_002349"
     CDS             complement(24791..25414)
                     /gene="yfaT"
                     /locus_tag="ACHJ12_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO4990125.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(25411..27099)
                     /gene="yfaA"
                     /locus_tag="ACHJ12_002350"
     CDS             complement(25411..27099)
                     /gene="yfaA"
                     /locus_tag="ACHJ12_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4990126.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQAQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(27248..29875)
                     /gene="gyrA"
                     /locus_tag="ACHJ12_002351"
     CDS             complement(27248..29875)
                     /gene="gyrA"
                     /locus_tag="ACHJ12_002351"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4990127.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            30022..30744
                     /gene="ubiG"
                     /locus_tag="ACHJ12_002352"
     CDS             30022..30744
                     /gene="ubiG"
                     /locus_tag="ACHJ12_002352"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4990128.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(30872..34606)
                     /gene="yfaL"
                     /locus_tag="ACHJ12_002353"
     CDS             complement(30872..34606)
                     /gene="yfaL"
                     /locus_tag="ACHJ12_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4990129.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSVSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHNPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         8863 a         9162 c         8225 g         8444 t
ORIGIN      
        1 ggcagcataa aatcatcctt ttatgaaaat caacgttttt tgagtactcg cacacaaaaa
       61 attaccgcac gggttgcaac gacaacctgc attttgcgga gcatatgaca aataaagtac
      121 gggtgcgttg aggatgcctg acacatcaga ggtggcgagg gatttctccc tcgcctggtc
      181 tcttacttct cagattcgta gtctacgaag acagcgacct ccgtctgacc ggttcggatt
      241 cgtacctcac agaggtcttt cctcgttacc agtgccgtca taatgacggt taaacagatg
      301 acgatcaggg cgattaacat cgccttttgc tgcttcatag actgcttctc cttgccttcc
      361 ggcacgtaag aggctaacct acggttgtgt gacatagatt gggcctcaga ttaatgttaa
      421 gcgtcttgca ggacgcgtaa tgttaactgg ggcttttctc cgtatgcctt acggcggcat
      481 gcccgaggca gacaccctca agcacccgca gtcattctac agtaaaaccc cgcgctttca
      541 atttcccgta tgtccggaaa actgactgac ctgtgcccct gatcgttcac gctcaacgta
      601 tctgtccatt tccagtttta ttccctgcat aatcaacatc ccctcaataa caccttcagc
      661 tttttgcagt acccgcccgg cccagcaatc agagcgctca tgcttacggc ccagtcccat
      721 aagcgtcatc ccgaacacat aataatcata cagaaaatca tgcaaatcgc tgttgttgac
      781 gttcagttgc gccatgcaat tgctgataat cagcgcatca tcatcggtac actgaggccg
      841 tgatttaacc tttgatggga tcactccctt aaatccggca gcgacgccgg accatgccac
      901 actctcatga ttatttacca cccatgcacc ccatcgggac agaacctgtt ggatatcacg
      961 cattattctg tactccgctg taaccatact ttttctgatt tgaatgctca gggggaaaat
     1021 gtctactgga tatctccggc ttcattgctc acactcccca accagattga gaatgaccgc
     1081 tgaaccattg tcttccatgt actcatcttt tccgcttgcc agaaaccagc gacacacttc
     1141 aaccgcttca tcacgcgtca caggatttat ttttcccagc aaattttcga gccagaactg
     1201 ccgatcatat actgactcat gatgttcacc tgtgaaaccg tatttgtccc ctgtttctct
     1261 ccccgctgtc tggcgcatct cataaagcca gtcccagtaa ataaactcac gaacaacgtc
     1321 tgacaatgta tgaggctctg gcagtacatc acgatagcca tcaacatatg cacgacgctg
     1381 atcatcaatt tcatttgagc gaacaccgtc aatacttccg gcttttttct ctgccgcagt
     1441 ccagccccag tagtaatcgt cgataaattt cggggaggtg cgaattatcc gctcagcctc
     1501 cacatcgtcg agtgctgcct catagctgcc aaatgtggcc ctgactgatg cggctttttt
     1561 gatattctca cgggcattcc tgatcgcctg cgccgggtta tccatgccta tggttgcgaa
     1621 agcaatctgg aaaggatcaa cattaatctc ctgcaaataa cgtccatacc gttcactcgc
     1681 ttcttccggc gttattttga gtttctccag tgctgcttcg gctgcgtcca gatgtacggc
     1741 ctcgttcagg tgaataacct ccagaatcca gagataagca tcagtctgtt tatgtccggt
     1801 gatcctcctt tgttctggca gaggtttgat atttgccgga acggcgctgt acgctgccgt
     1861 cgggatagtg aataaagctt tatgtacctg attgtccgta cgcatcacgc aaccgccttt
     1921 tttctgtaaa aaaccagttc ccgaacctga tcgccgttca tgagcatatt gttgaaatca
     1981 tcatgatcag gccaccggac acttacccgc tgaatgtcat ttcgggcctg gagatttgca
     2041 tgtgcgcatt cacaggccgc agccattccg gtagcactgt attcatcgcg atcggcaaaa
     2101 ataatcagat gggtgactcc cgccggtacg cggaactttt tcataaaacc actgttaatg
     2161 gttgcccagg tatttacttt gtaaatctga tgtgcggaca acgcggtttc gataccttca
     2221 gctataccca gtgtactggc taccggaaac atcctgatag caactgaccg ggcatggtcc
     2281 agatagttat cgtcctgtaa agatttgagc cttctggcgc tgataccaat gtctgctttt
     2341 ttagctccat caagcaatgt ctgatgcagg taacacagtt ctcccttgtc atcggtcgcc
     2401 agggcataaa gagactggaa catgcgcccg ttataatttt gctggctgtt gaagcgaact
     2461 ccctcaaccg gaagactgaa tatgccacgg gaacgaagat attcagcacc agatgttccc
     2521 tgtagcgccg tgagcgctga aaatcgacgc tgaacgcgcg atctcaggct ttccgacgta
     2581 cagatcacag gcggtttatc tctccggtat tcattccccg tgatgcggtc tatttcagcg
     2641 caaatttcat gaaacgctct tttttgtgtc agagcgatca gtttcatgcc gtcaccgcta
     2701 ccacaaacac agatccacgt tccggtgcca tcacggtcat caatacggaa cttacctctg
     2761 gcaccacaaa ccggacattc ccctttgaag tgctttttac ccgtgactgg cggcagtccg
     2821 taatgtttta aaatttcagg ccagtggcct ttcgctgctt ctgctgtttt catcttactg
     2881 actcagactg tttctgtttt tctgaagttg ttgctttgcc tgcatgatct gccacgactc
     2941 actgccttcc ggcaccccag accggacacc atgaagcagg gacagagaaa gcgcttcggg
     3001 ggatttttcc gtcatcttct ctgcccggct acgccctctg gcaaaccgga tgaatttgtg
     3061 tctgatgtaa ttactgacct ccggggtgat ttccatcgga aaactgctca gcccgtcagg
     3121 ccattcgccg aacttctccc ggaaagtgtg agcgcaccat ccatcgctga ccggtcgccc
     3181 cagagaggca cgctgacgct gatagaattt gatctgactc caccaggcct gtttgtcgct
     3241 tttggtgtaa attttttcgt ccttgctgag tttcctgata ttgcggcggg tatctgtgtt
     3301 cacatcctca ccagccagcg gtttaaatcc gcatttcgga cagacataaa cacctgccgg
     3361 tttcatgaaa tgacaccccg gacattcttt cggcagtttt tcttcccgaa tttcgttacc
     3421 acgtgatgat gacacctgca tcccgtcgtt tttgccgggc agttcgtcat actcaatatt
     3481 ttcaggaaat cccaggcgat gaaccgtacc ggaatggtcg aaaatcaggc atgactcctt
     3541 gcctggcgct gtacgcaatc ctctcccaat cgcctgaagc cagcgaatct ctgattttgt
     3601 cgggcgtgcg taaatgatgc accggacatc actgtcaaat cccgccacca gaacgccaac
     3661 gctgacaatg attttcgtcg ctcccgtttc aaaacgactg atcattaact gacgctcatc
     3721 atgtggcgtt tcagcaacca tcacctctgc gttaattccg gctttgttga actgcatggt
     3781 cacgaagctg gcatgcgttt tattgacgca gaacgccact gtcggtaaat cccttccgtt
     3841 acacagccag ttatcgacaa tatcgccaac cagatccgcg ccgcacataa tctctgccag
     3901 ttgtgattcg tcgtaatccc tgccgtactc cattgagact ttcgttttta caccttccag
     3961 atccggtttc gtgggcgcat aaaattcgta attgctcagt tcgccacgct ggatcagttc
     4021 ccctatagtg gtcggcttga gaagacgctg gtaataacgc cccaggaacg atgaaaaggg
     4081 tgtaccggac aacccaatca ccttcacatt tccctctgca accagtcgtt cgatttccgt
     4141 gaggatgcgg cgcttacgga gatgcgcttc atcaataatc agcaggtcga tattctgagg
     4201 aaaatcacgg cgaatcagcg tatccgcact ggcaatctga atcagtcgtg acgggtcgtt
     4261 attgggatga tcacgccaga taaagctgat ttcatccccg gaaagcccgt actgaacaaa
     4321 gcgctgtgct gtctggttga tcaaaatggt ataaggaacg acaaacagaa cgcgcatgcc
     4381 acggcttacc agaccagcag tgatgaacgc cgccaggcca gttttaccac tccctgtagg
     4441 catgtacatc atgaaggact gatatgtttt ccagtcatga cgcaacatat tcagcgcacg
     4501 ttcctgagca aaattcggct tgatattcag catggtattc tcccgatgac agggctgata
     4561 cagtgattac ttaaaccggg tcgcccggat gacatccaca ccatcggcga tataacgctg
     4621 tggatgtttg ccatcacgga gaaaaacaaa tcccgaaatg ccttcaggaa gcgctcgaat
     4681 tttcatcagt gaacgctgac gaggcgatcg gttatccacc tcaactgcgc aacgctgacc
     4741 atcgggagaa ataaccagaa gatcgataaa tcccttgcga tcacctttca gcctgacagt
     4801 gaaacagcgt tgcaactcac agcccaggga tttgattttc ttttgcagaa cgtagtcgaa
     4861 tgtgaccttg tccattccat gtccgaattc ctcttccaga aggttgatta gtaccagttt
     4921 cagttcagca ctcatatttt aaccatgatg tactagtttg ctgatgcagc cgttttttca
     4981 ggggtgcccc cctttagatc gagatctacc taacctctgt acccgtctgt tggaagatcc
     5041 cgttccagtg cttcgcacta acacacgggc actcccccct tacccccctc tcaacttatg
     5101 tactagcttg gtagtacata agtgcatcct gagaagtggg gtctcagccc gtcggtcacc
     5161 tttaagacca acaccattca ggtacgctgt tgcgttcctg ccaggggcgg ctgagtcgta
     5221 tacccctgta atgcacgccc gtgtgtttca acgaacctgc gcagccgtac attggcttca
     5281 tgcctcgctc tgttctcctt acggaatgaa acaggctcgg aatcaaaagt aacttcgtaa
     5341 acctccgcgt atttcagggc gaccttctgt ctcagtgagg aaggcaggct ctgtaactgc
     5401 cgcttaatcc actctgaatc tgcctggctg tacctggcag gcatctccat ctgaacgtaa
     5461 tcaggggaca tacagcctcc ggcagaacat cacgattcat gactggcatt catgagcgac
     5521 ggatgctgat aaggaatttt gggatccaga tgacaaagga tcgccacgtc ttccggcaca
     5581 cctcgggttt tccactttcc aacgccctga ctacctctag gttttcctct cttagggaaa
     5641 cggcgaccaa ttgcagcatt ggttttaaac tcagctttta aaatttcgta aagagtcatc
     5701 tctcactcac tgattgatac tttagcgtcc ataaatgtac aacaaatgaa tctaaagtat
     5761 caagaatatg tgctacttta gtatcgaccg aaaaaacagg agagataaaa tgagaacttt
     5821 gggagagcgc cttagcagcg ccaggcaacg ggcaggattg acacaagatg cattagcaaa
     5881 aaaagccggt ataacccgcg tggctataag caaagcagag caaggactta ctaaaagctt
     5941 caacggagat actttattca aggtagccac agccctaaac tgcactccac aatggctaca
     6001 aaatggagaa gaacaatctg taaactggga aaacaatgtt aagagttgtc ctcaaaaaga
     6061 cgcatttcat tcctatcccg ttattaactg ggtccaggcc ggactattct ctactgctgg
     6121 agatgattac agcatgtatg accatgataa ctggagacat tctgtcaaat atgcaggtga
     6181 aagaggtttt tggcttgaag tacatggtga ttcaatgacc tcgcctgttg gaatcacctt
     6241 tcctgagggt atgtctattc tcgtaaaccc agacaaagct gtacattcag gctgttacgt
     6301 tatcgcaaga aaaaaatcaa ccaacgaagc aacattcaaa aaatatgtga ctgaaatggg
     6361 aagatctttc ctgaaaccac tcaatcctca gtatcccatc attgaaatag atgatgattg
     6421 tgaaatcgtc ggcgtgatcg tggatgcccg atgggatatt ttctaagaaa gatactttag
     6481 tatcaaaaaa cgcttgccca tgaatgatac tttagttacc atactaaaaa agtaactgag
     6541 gtatcattaa tatgttccaa aaaactacgc tatcatgcat tgaagattta aaaaatcttg
     6601 gctccctcat tgctcttatc gcaaaagcag cgccagatac aactcttgcc aatgatatcg
     6661 agtcatgcgc cggaatggcg tgggatataa taaacaaagt atctcacaca ttatctttgt
     6721 taattcataa acaaaacatt tctgattgcc cccatgagat ccgcacccaa cgcgaaagct
     6781 gcggtctatc caccgctgaa ctcgccacgc tgcttggagt tgatgaagaa accatcatcc
     6841 agtgggagaa cggcaaggct gaacctctgg caagccaggt gattcctctg gcaaatatac
     6901 ttgactgcga tccgatgtgg ttacttacag gtttccattc tgacactaca aaggaatcgt
     6961 aagatgaaaa tgttcaaagg cctcaccaac gaaccggaaa cagcttttca tcacattgca
     7021 gtactgcttg aagcggggtt aatcatttcg gcttccggtg atgaagaatg tgatgaactt
     7081 tcggatgaca tctttttact ggcacaacaa tacgccagaa gcgcatgcga tgcatttaag
     7141 gaacaaagaa catgaaaact cccctgatta tgcttgagga agtggcagca gaaataaaag
     7201 aaaacacgtc aatgcttgag tttatattta aaaactctgg tgataacgga gagacagatg
     7261 atttcttact ttgtctgatt cgttccatga ataaaacctg tgaaaaggct tacgagtatg
     7321 ttgatgctct gagaacgaat aaaagaaatt aaacagacaa tcatacataa aaacatgacg
     7381 gtctgcggtc gggagttctc actattttaa aatggggatg acacatgaaa gacaaacatg
     7441 cctataaaac cgctttatta atggctgatg ccggatactg gtcagttgca gtattgtttc
     7501 tcaaaaaggc atacgggaaa taacaaatga aagatgaata catttctgca tatcgtcgcc
     7561 gcataacggt tgcagcactg cggcggatga aacgaaaaac aggaagtaat ctcctcactg
     7621 taaagcgtca tgatggagag ttaacaacta ttgaaataac agaacagttc ataagccagt
     7681 tgctattacg atttgaaggc gttacccgtg gtgagtttgg gcggaaagat ggtgaaacag
     7741 caatacgcac tgcataccag gatgctgtcg gtatcaacca acatggtgag tatctgactg
     7801 aatcaggaaa gttaatcatt gatgaacttt tgaatgaatg catcgattac attaaagagg
     7861 ggcatattac cggatgcgtt aatcattaac agcatagctc ctctcccgga ataacggaga
     7921 taatgaaaaa tgaataattg cgttgaagta ctcgataatg tccactgcga gataacagaa
     7981 agcctgtctt tgttacaggt gattattgac agcggtgata ttggttcacc agatgcgcta
     8041 aacatgatga attgtctttt ccgctcatta tcctgtacag cagacaaagc agaaaagcat
     8101 ctgtgccgga ctgaaaacga ataacattat ttaagaacag taattaattt acagctttat
     8161 tgtcggggat ttttacaacc tgaaaaggcc aggctgtatc acagatatat cgcaacaaac
     8221 gaattgctga gccacatctt ctggctgcgc tcagagtgaa taaaagtaat taacaccgga
     8281 tgaacacgga gtaaccatga aagaagaacc tgaaatcatt gaaattaatt ctgaagaatt
     8341 cgcagaaaac gaatggctga cagaaaccga tccgcgcgtt ctggcgctga caatggaagg
     8401 atacatcgta tttccacctt tcttcagtga tgcggaatac agtgcaataa ataatgccgg
     8461 aatcgaccgt gcttttcata aatacgaact cgattgcggc cctgtttaca gagtctgccg
     8521 ccacggacaa gttaagaaat gcttcacgcg aaaaacggcc atcatacatc tcgctcattt
     8581 tatgacaacg aaggtattcg aaagttcagg ttttgaaaaa cgtctgcctg accagcgcct
     8641 gatgcatccg gaatatggag aagtctttca tccgggagaa attacgcccg aatactacag
     8701 agctcacggg cgttgttttc atctcctgtt gcgcatcctt gcacgcagaa aacagtttga
     8761 tgcctggatg gaaaaataca acgcctggat tgatcagtat tgccggcttg tcgcgcaaag
     8821 accatttaat aagagagcaa accatgacag caactgaaca gttatttttt cttactgctg
     8881 aaggtatcca cacaccttac ggactgcttg atatcaacaa agcactggaa atgctcgatg
     8941 aaggctgttt tgacgagccg atctggcatg gtctggaaat agtgtgttgc atcagtgaag
     9001 cagaagaaaa aggcatgaca ggtgagctag cgccggaaaa gacaatcatg tactggcgct
     9061 ggctttatac cgccatgttc ttctctgaac agctcaccag aaacggctgg accgaagtgc
     9121 ttaacgagaa tggcggtaca gacaagggca ccatgtacag aggccacaac ggtggcatga
     9181 caatctacct gaaaccatta cgtctcgcta tgcagaacta tatggaatgg gcactcatag
     9241 aaacttacgg agcggaatca gggctgctca aaagcattct tatgtacaaa gcattcaccg
     9301 gcgctctggg cgacggcgaa aaaaccggca tcactatgga aggttacaga gcgatgcaca
     9361 tgattctgga tgggcttatc agtgaagcca ctgacaactc agaaccggaa accagagtgt
     9421 tccactaatc agcttttcgt gaggtgtaat gatgaaaaaa tcaactgaac ttgtgccggt
     9481 ggcagcccgt gatttgcaaa ttatcgaata tcgcggtcaa cgcgttgtga ccactgaaca
     9541 actggcggcg gggtatggaa caacaccaat ccgtattcag cagaatcacc atgaaaataa
     9601 atcccggttt attgagggga agcacttctt taaagttgtt ggtgatgaac tcaaaaattt
     9661 gcgactagtt ttaaacgagt cacaaaatga gatgaaaata tctcccaaaa ctcgctccct
     9721 catcctctgg accgagcgcg gcgcagccaa ccacgcaaaa ttactggaaa ctgatcaggc
     9781 gtggaactat cacgaagacc ttgtggagtt ctacttcact cagcgagaaa acatcgatgt
     9841 tcagcgcgta ctcactcgca aggaactcgc gttgatggtt attgaggctg aagaacgggc
     9901 tgaagctatg gtcctggaaa ataaaacgct ctctgcaacc gtagacagtc tggaaaaaca
     9961 tttctctaaa ggcatgactg ttccccagtt ctgccgtgcg ctgaacggcg taaataccag
    10021 ccagataatg tggtggctgt ctggccgaaa atgggtattc aacgaacaac gcgatccgga
    10081 gaaaacaccc cgctggcgta cggcctctta tgcccgtgac agatacctga ccgaagaagc
    10141 agtacagatc actccacacg ggaaagcaac attcacgaaa ttcacacccg tattgctgga
    10201 aaaaggatgc caccgtttat accagtttta catgaacggt gaactcccta tgaagaaaaa
    10261 ctggaacggt gaatttaaac acgacaaagc tatttacaca ccggaggcag aacatgaata
    10321 acactactgc ggcaatcatg aactacgacc cgaacatgac attatgcggg cgcatggcaa
    10381 gacagacggt tcgtctgaca ctcgggcaat gggaataccg cgaaacattt gaagtggacg
    10441 ttgtcggcaa tctgacggga ctggatgtaa taacctgcgc aattgaaaac ctttatgaag
    10501 acctgccgta tgaagaagca cagaatgaat ataccggtga aactaacatt atggccacaa
    10561 tacaggtcgg tgatcttgca tgtacggatg agggcatgct cggcgaagca tggctggcag
    10621 atatgcttat tgcagcagaa attatccgca ttgaaccagc aggaacactt tcataacgga
    10681 gatcgccatc agatgattat ttcattcacc gattacggcg aacaggcaaa actggttatc
    10741 accagttccc gcctgacatt gcgaaaacat aatcgtgtcg ttgatgcggt acttctgtgc
    10801 acgccggaca tcacagaaag ccggagcggt tttttcctag ttaagtcgtc attatcagga
    10861 aaaacacgcg acatcctgcg cgcctacaaa acagcaacaa gggagtctga acgatgaaag
    10921 aagctgatct gaaagcactg cgtgacgccg cacaaaatac cgtatatggc gacaagggat
    10981 gtgatctgga agcatttcac tcccttgcca ctccgccagt tatccttgcg ctaatcagtc
    11041 acatagaaaa tctggagaaa aaactggatg aaatcgtaac gtcagagatt agcccgaagg
    11101 cctggctgaa taaccttacg ggggaagttt gcagtattga tgccatgcct gacgcccgta
    11161 atgacagaga aacatacacc ccgctttatc ctgcttggca gcgcattgcc gccgcagatg
    11221 ataacggcag agtggcatgg atgtatacgc acaaaaaagc aggttacagg gtccccacaa
    11281 tgacatcgtg ggaaagtctt gcagaagagc ggggaggctc tctggataaa tatatcgtca
    11341 gtccactatt cactgttcct cagataccgc cagtgagaac agaacaggat gcttaccctg
    11401 atgtgcaggt ttcatgggat gcaacacgtc actatgtcaa cggctggaac gcctgccgac
    11461 aggcaatgct gacgacggca gaaaaagtac ttgatgtgcg aatccctgaa tatatcggac
    11521 gctggctttc cgctgcactt catgatcaga actcatgcga ggaaatgaaa aacgatatac
    11581 gacgcttcct ggctgaatac caccatgttc catcgaaata taacggctgg cgacttgtac
    11641 cggacaagtt aaccgaacag atgaagtacc ggattgtgcc actactggcg accatcaagc
    11701 cccgcagcgt cgatgaatat gaccgctcag gagaaaacgc acaatttctt gactgggtat
    11761 gggcgcaact gatggaagcc gcgaaattcg cgtatgaggg ctcagtgaat gggtgaaatg
    11821 gttgatatta acgatgaact ggtccgcaca gtaattaccg ttgatgacgg atgcgaccat
    11881 acccagcgtt ttatctggat gatgaataca cggcgtaata tcagaaacaa agtgttccgg
    11941 cacgaaccaa agccagtcag ggtgacaaag atacgcattg aagccagaaa acgccgcaaa
    12001 tacaggccac ggaaggccag ggccccagcg caaccggatg aactggcata aagacagcag
    12061 gagaataagt cgtgaatcgc gagtttgaaa tatggatacg actcagatac gggtaccgat
    12121 acgatttaac cagagatgct cacggcttct actgccgtga agttgtaaag cgaatgtttg
    12181 acatgtggtg ccactgccgt ggcctgaaga tagtgtgagt caggaatgga aacaatcatc
    12241 tatcagataa cgcccagcaa atggtgcaca gaaagaaccc ttattgccgc aactggactg
    12301 aaatcaggaa ccattgccag agcaagaaaa aaatcctggt tactggggaa agaataccgt
    12361 cattacgcag cagaaggaga gcccgggcct ttcagtgaat gtgtatacaa cattgaggaa
    12421 attatgcgct ggatcgaaaa ccagaaacaa ccaggtgcca aaaatgcaag ttccggttaa
    12481 cctgttaatg ctcctggacg tctgggaggt ttaatgagta acgcatcata cccgacaggc
    12541 gttgaaaacc atggcggatc actccgcata tggtttcact ataacggcaa acgtgtcaga
    12601 gaaaacctcg gtgttcctga caccgccaaa aaccggaaga tcgcaggtga acttcgcact
    12661 tccgtttgtt ttgcaatcag aatggggagc ttcgactacg ccgcgcagtt ccctaattcc
    12721 cctaacctga aacattttgg tctgggaaaa aaagagataa ccattaaggc actttcggaa
    12781 aaatggcttg agcttaagaa aatggaaata acagcaaatg ccatggtcag atacaaatca
    12841 gctatccgaa attgccttga ctgccttgga gggaacaaac tggcatcatc aattacacag
    12901 gaagatgttc tcatttttcg taaagaatta ttaacgggat atcagttgcc aggaaaaaat
    12961 cagaggaagc ctattaaagg tcgtagtgta cctactgtaa atagctatat gacaattatc
    13021 tccgggatgt tccgttttgc ggcaaacaat gggtatatag gcaaaaaccc tttcagtgag
    13081 cttccatcac tgaaaagagc gaaaacacct cccgatccac taacaaaaga tgagtttgcc
    13141 agactgattg atgcgtgcca ccaccagcaa actaaaaatt tctggtcgct ggctgtatat
    13201 accgggatgc ggcatggcga actgtgtggg ctggcatggg aggatatcga tctggaagcg
    13261 ggtaccttga ccgtcaaaag gaattacact cagacaaagg aatttacact acccaaaaca
    13321 gatgcaggaa caaacagggt aatacaactc atacagccag caattgacgt attaaaaaat
    13381 caggcgacat taaccaggct tggtaaacaa tatcagatag aagtaaagct gcgagagtat
    13441 ggccggacaa gcactcactc atgcagcttt gttttcaatc ctcagatatt agatcgcaca
    13501 ggtaactcag gagttcatta tgccgtaaca tccatcaaca gaacatggga atctgcatta
    13561 aaacgggcaa aaattcgcca tagaaaagcg tatcagtctc ggcatactta tgcttgctgg
    13621 gctttaactg ccggagcaaa tccaaacttc atagccaacc agatgggaca tgcgaatgcc
    13681 cagatggttt acaatgttta cggtgcatgg atgaaagaca ataaccagtc ccaggtagaa
    13741 gtactaaaca gaaagctatg tacttatgtc ccacaggtgc cccaacgact aaacaaagca
    13801 tagaatatat ttttaaaatc aaatagttaa tacataggta caagtacatg aacaatatga
    13861 acgtaattat tgccgatgac catccgatag tcttgttcgg tattcgcaaa tcacttgagc
    13921 aaattgagtg ggtgaatgtt gtcggcgaat ttgaagactc tacagcactg atcaacaacc
    13981 tgccgaaact ggatgcgcat gtgttgatta ccgatctctc catgcctggc gataagtacg
    14041 gcgatggcat taccttaatc aagtacatca agcgccattt cccaagcctg tcgatcattg
    14101 ttctgaccat gaacaacaac ccggcgattc ttagtgcggt attggatctg gatatcgaag
    14161 ggatcgtgct gaaacaaggt gcaccgaccg atctgccgaa agctctcgcc gcgctgcaga
    14221 aagggaagaa atttaccccg gaaagcgttt ctcgcctgtt ggaaaaaatc agtgctggtg
    14281 gttacggtga caagcgtctc tcgccaaaag agagtgaagt tctgcgcctg tttgcggaag
    14341 gcttcctggt gaccgagatc gctaaaaagc tgaaccgcag tattaaaacc atcagtagcc
    14401 agaagaaatc tgcgatgatg aagctgggtg tcgagaacga tatcgccctg ctgaattatc
    14461 tctcttctgt gaccttaagt ccggcagata aagactaatc acctgtaggc cagataagac
    14521 gcgttagtgt cttatctggc atttgcaccg attgccggat gcggcgtaaa cgccttatcc
    14581 ggcctacgat tcccattatt tcaacagatt acattaaagt aggccagata agacgcgtca
    14641 gcgtcgcatc tggcatttgc actgaatgcc agatgcggca taaacacctt atccgtccta
    14701 cgattcccgc gatttcctga ccctctcggc atataccgtc agcgtctgtt ttatcacatc
    14761 cagcgttacc ggcttcgaca ggcagctgtc cataccggac tccagacacc gctgcttctc
    14821 ttcagccaac gcattagcag ttactccgat taccggcaac gtcagtccca actgacgaat
    14881 gcgttgcgtc aagcggtaac catccatatt tggcatgttg acgtcgctaa gcacgatatc
    14941 aatatgattc ttgctaagta cattaagcgc atcgacacca tcattcgcgg ttttacattg
    15001 atagcccaac gatcccaact gatctgccag cagacgtcgg ttaatcggat gatcatccac
    15061 gaccagaatc atcatatcgt cattatcgct gaccgctttg tccgtcgacg gcagagcgtt
    15121 agcaggatcg tcgctctcca tctcgatcaa ataaatacgc gccaacaatg ccggtagctc
    15181 atgcggggca gccacactgt gtacccactc ccctggcgct ttctccagcg gaataccaat
    15241 atggcgacga cagaaggtca ctaccgctct accctgccat tttttactca ctacctcgtc
    15301 agtaattaac acatcttcgg gagtcggttc ctgcccttcg tatgttgtaa cgacgatgcc
    15361 gctgcgctgc aaactggttt ccaggaactg acagagcgac gcattgcgga ccgccagcca
    15421 gcagcgttta ccactcaacc cttccacgcc ttttttctgc gggtactgag cgccgtacaa
    15481 cggaatacgc acggtaaact ggctgcccat tcccggttct gaatctaccg agatatcgcc
    15541 gtccatcatg ctgatcagtt tttcacaaat cgccagaccc agaccggtcc cctggaagtt
    15601 tcgctgtacg cccgttccga cctggaagaa gggatcaaac aagcgcacca cttctttcgc
    15661 cggaatcccc acgccggtat cgcgaacacg gatagagaga taatcgccat cagcgcggac
    15721 atgcaaaact atacagccgg tatcggtgaa ttttatggcg ttactcaaca ggttggagat
    15781 gacctgctgt aaacgcatcg ggtcgccatt taaggccact ggcacatccg gttcaataaa
    15841 gcagtacaag cctaactgct tgcgtaccac cagcggtaaa tagttggcgg tgatgtggtt
    15901 catcacttca cgcggtgaaa actcacgcgg ttcgatcttc aactgttccg attcaatctt
    15961 cgagaaatcg agaatatcgc tgataatttt caacaacagg ctggaagagt tgttcattgc
    16021 cgtcaccagc cgatcgacgc ctttcggtaa ctctttggtt tgcaacagat ccaggttacc
    16081 gataatgcca tacagcggcg ttcgcagctc atgactgacg gtggcaagga acatcgattt
    16141 tgactggctc gcctgttccg ctgcttgtgc catctcctgc aacgactctt ccatcttcac
    16201 gcgcgaagaa acatccacca gcacacaaat ggccacgttt tcattacgat agcgcgaatg
    16261 gacgaagctg atttgcagat tggtattgtt gctggtcagg acatcaacaa aattgacctg
    16321 ctgcccacag ataatttgcg tcagtcgttg gcggtcctca tgcgtaagca tattgagata
    16381 ggtatgcgcc agttcgttac ttaaaatatt gacgccatca gcggtacgca aaatgcagat
    16441 acccactggc gcggaggcga caatcttgcg attgaactgc tcatgttctt ccagtcgcag
    16501 ggcgtcgctt tccgccggaa tgaaaatacg tcgctcgtac atccgcgcga gagtaaacaa
    16561 tgcagctccg gcaagcacat tcagcaaaat tgcgttaagg atcaacatgc gaatgcgttc
    16621 cagcacctta tcaaccggca ccgaatacac gatgcttagc gatgagggtg gcagattttt
    16681 cttcagcacc agctcccgga acccttccgt atagccaaac caggagcgtt cctgcatcca
    16741 gcgaggatcg cccttaattt tactttctgg tccggtaagc gaaatcaggg tatgaccatt
    16801 ttcatcaaga atggtaaccc ccatcggcaa cgtacccggt aagaaaaagt tctccatccg
    16861 gatggtctgc tcgacaccca aaagcgcctg caaccggttc gccagataaa ctggcgtcaa
    16921 cgcgtaaaaa tatccgacgc cagggcgcgg accttcgctg atccagtaga ggttactgcc
    16981 gctatcatct tgtggtgcat ttcgatattt attgatgcgt tcatgcaaag ctttcaacgc
    17041 ggtatcgcgt tccactggca tatcacgcag accgaaattg gccatgcaga ggttatcgct
    17101 gccgattaaa aatacccggt tgagatcgta agccgcagaa aaattatcgc gccagtagcg
    17161 cataaaccac gccaatgact ccagagaacc tcgccaggtg ttactcattg cggaacaatc
    17221 ggagtcggca aacagcggtt caaacgcagg cacatccgcc tgcgtttctc gtccacgcgg
    17281 ggaaagcaca ccgttttctg ccgataagcg attttcggcg atgtacttca gctctttcat
    17341 cacatcagag gtgcgttgaa taaagcgctg agcctgatcg gaactcagat taaattcctg
    17401 acgaatttcc gattctcgct gatgtaacgc attaacgatg taaaaaacgg atgaaaaagc
    17461 aatcaacagc cagagcacta acgccaatgc tctgaacatg tagcgcgagg ctttcagggt
    17521 tgtacgaaaa gaagcaaggt atttcaaagg ggcgaagctc cgcctcaggt gaccgatgga
    17581 gtgtggttaa ggtagcggta aaagcgtgtt accgcaatgt tctgtcacca ttgcaataat
    17641 gcgtatcgct tagaaaccat tgccttaacc aggaaagccg gagtagatgg acagttctca
    17701 gtacgaaaaa taagaaatga ccaatctgcc gacgcttgtt aatggttata tcctggcgtc
    17761 ggcagatcgc ccagcagtct tatctgaaaa aagagagagc caggttgtgt gaagggatgg
    17821 atcaccagtc aaaacttttc accagcgtca gctcgccagc attacgcatc ggtacaataa
    17881 atgtttcctg tttctcattg accgatcctt catcggtgat cagcgtcagt tgggcggtcg
    17941 tcaattccgt ttcgctgcgc ccaccatagt agttgatata cacctgatag cgcccgtgaa
    18001 ctggtgctgg catggcgaaa atctcgggtc cgtaccccgt cgtgacatcc atatccagtg
    18061 caccactgtt tttcagcacg gtgttaccgt accaggcgtg ttcgccatcg ggcgtgacaa
    18121 catggagatc gagatcggta ttgtccgtat cccacgagag aaccagccgt aaacgtgcac
    18181 gaatcgttcc tgtacccggc gttgagtaaa actgcatttt ttgtcggctt tgcccatccg
    18241 ggctgatgac ctgcacgctg ttgctgcctt cagtgaaaat ataagggcgt gcaaatgaac
    18301 catcggattc aactctctgc ggcatactgg caccgttgac caccagccgg ccctgctgaa
    18361 ctttacccgc cgcagcataa ttctttattt tgccgcggat ctgcgctgag atattttgat
    18421 catctgccat gttgacggac gacgccgggt aattgatcga ttgtgaaaaa ctggcatctt
    18481 cgccctctgc cggatgccag ccagagagcg gtgcgtcaat ctcgacttcc tgcacacctt
    18541 cactgtgagc gacaggcgaa agtgccacca gtaacaacgg aagaaaaatc tttcgcatag
    18601 cattagtcca ttaataattg tcgggtcagc gtttcgatat agttctcatc aagcccggtg
    18661 ggataaccat caaaggccag gtgcagatac tcatgggtta aatccagccg atcctgaagc
    18721 gtaaagaagt tacgaataaa cagccgtttc tgctgccgat cggtataggg gaaacctgag
    18781 actaaacgac agaccgcaaa cacgtctggt tcgttgtacc ccgtctcgcc ttgtaatata
    18841 cgccgccact gcggcatttt tttcgccagc caggctttcg ctttgggtaa taactggcag
    18901 gttgagcgtg gagctcccca gcggctaagg ctgttgtcgg gataagcaaa cgcgagtatc
    18961 tggtcgtaac gctccccctg ccccgcctgg gccgtggctt gccgccagga aagtgtccct
    19021 tcggtagcgc gactgccatg ataatgaacc ggatcgcctg catagatgag atcctgcgtc
    19081 caggcggtca tcgttcgcgc ccccgtggtc gctggcgaag cagaaacacg ctgcgtggcg
    19141 ctgctgtcgg ggatggtcag gcaatcgcct tcccgattgg cgttttgctg taaaaaagtg
    19201 cgaatcgcca cggtcatggc tttcgctgcc tcagggggcg tacttttcgc ctcgcgatcc
    19261 agaactctgg caacgtactc ttcacgatcg agatgcgact gcaatttcag tttgcccttt
    19321 tcgctcagta aggtcgtctc accgtggctg acaaacgtaa tgtggttacc attggtaaac
    19381 gtcacacgat agcgcccatt aagtacgccg ggttttactg ccgtcgtact cttttccgct
    19441 gtgattttct tcagcggata gcgggcaaac agctccacct ctacacattg cccactggca
    19501 acctgcgtcg gcactggcaa aacccgatta agcaccgtcg cataacgggt tagtaccgtt
    19561 ttgctggtgc cactgccagt agcccacagc ggtgtaccat cggttaacca gccggcaaaa
    19621 ccaccctgac ggatttcctg tttatcatcg gcaaaccagc tccaggtttt cacccgtacc
    19681 cgactaccga gcatggatgc gactccgatt ttcgcctcgt ccagcaccac atccagtaag
    19741 acttcctgag ctttgttttg cgcaggtaac gtcgctaatg aatcaagcag cgatttaacc
    19801 gttaccgagg tttctggctt cagcgtagta agagacgcta accacgcagg tgcctgacgc
    19861 tgttgccagt attgccccca cacatctgcg ccaatatgca accgctgcgg cgcaaaatag
    19921 agcccacagg aacgtaccag cgcggtatcg cgcgttatgc tctccccagc ctgacaacaa
    19981 taaacctctt ccggggagtt tccgcgacac tggtagggct gctccggttg atgggtatcc
    20041 tccagccagg cgtaaacata cagtttccac aaactcccta acggcgtggt gagcgagtca
    20101 ggtaatgtcg aggctttggt cacgcctgat gacgacagct gatacagttg atcgtgctgc
    20161 gcaccgcgca gcaccagttg taacggcgtc tcctccgcaa gcgttggcag agtaaccaac
    20221 gccaatagcc agacgattct gcgccagttc acttatttca cctgcatccc ggtccattca
    20281 ctccctgccg caacactttg ctgcgcaggt gcataggaac gcacataacg agcaggaggc
    20341 agaacgaatt gccctttttg cgagaagcgc agcaaatggc ggaaagtgac cgttcccgtc
    20401 agttctttca ccggcaccat ataggccagt tcgcccattt cattacgcgc tttttccagc
    20461 aattgcccct gctgtttcgc ggcgttgggt ttattgactg aaatgcccca tgttgtgcgc
    20521 tcaacgtcgg ctcccggcgg gagcggtact tccacctgac catagcgcag aactgcatcc
    20581 tgctcgctgg taagcgtaat ttcatcgaga tacagcgcat cgctgtcaat ctcattgctg
    20641 gtcaccggtt gcagaataaa gctcatctct tcttcaccag ggataagccg atacaactgg
    20701 cgttcaacgg tcaccggaat gttactttgt tgagccattt ttgccggctc acgccagcgg
    20761 acctgcacat tttgcggcga taattcgtca ccaaaagaga gaatgtccgg cacgccctga
    20821 ccaacccaac gccagtcttc gccccctcca gttaacttat gtttagccca tgcgcccgca
    20881 ggcgcaggca aaacaactgg aggcatcgtc gccatatatt tcgccagcca gttcatggcc
    20941 agcgcgcgct caatagtgga ttgctcagcg gtcaaaccac ttaaaatagc ggccgtctgg
    21001 gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg attgctgttt
    21061 gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt caactgcgct
    21121 tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg agattccggt
    21181 gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa atcttcatct
    21241 ttagttccgc gccgggcgat agcttcatcc aaccctttca acaacgtttt gcacggcaga
    21301 ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat gttatccgcc
    21361 tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt tacgccgagc
    21421 gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc gtcaccattg
    21481 ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat cagccgcaga
    21541 cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg atcggcaagc
    21601 gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac gccaccccat
    21661 ggtccatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt ttcactactt
    21721 tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc accgccgagc
    21781 atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag ttttgtgcta
    21841 atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc agattgctga
    21901 atgttctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt ctggcgcatc
    21961 tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg actgaagata
    22021 aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact ccacttcatg
    22081 tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc gtcgccgttc
    22141 atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa cgtgaaatac
    22201 gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc cacctcttca
    22261 cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagttgc gccaggcgca
    22321 accggctcgc tggagagcgc ctggtcgtag ctgataaacg acagactgga gctggtacgc
    22381 acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat ttctggttgc
    22441 agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac aggtttacct
    22501 ttcagcgacg aggttaattc gacattgacc agttcaccag gctggtaatg ggttttgtcc
    22561 gttttcaccc ggatatcaag ctgaggaacg gcaactttga tcccggcgtt ctgaaaactg
    22621 tactgaccat tacgggtata cagcaccgaa aaagtgatgt taggcgcaaa ggaattgctc
    22681 actggaaccc gggcttcata ctgggtatcg tttaaacgtt gtaacgtcag ccagtttgcc
    22741 ggatgcgaaa gcagcgactg ctgttccacg cgatcgcgtt ccagcgtcaa taatgcttca
    22801 tcaattggct ccggaaaggt aatcagcatc ttcgcagttt cgcctggctg gtacagcgtt
    22861 ttatccgcca cgatatctac cgtaccagta tgcgccgtgc tgcccttacc gctgacggca
    22921 tgacttaacc cagcgagaat taagccgtct ttatcgcgta atgtcagatt gtagttgcca
    22981 ggtttagcga aattgacggt aaaggatttg ccgcctgacg gtagctctcc gctatggctc
    23041 gtgcggtctt cgagacgcaa ccattcatac gtaacaggaa cctgttttga agattccagc
    23101 gcggcataac ggaacacaac cgactcgccg ctattactgt attgtgcggc ggtacttaaa
    23161 gagtaatgcg ccaggccgcg ttcaatgagg atctctttgg tggtggtgac gcgatacgcc
    23221 gcgccgtcac tggcggagac ggttaacaaa tagcggctcg gtttatcggc ggcggggaga
    23281 tttaacgcca catgaccgct ggcgtcggac accgtttcgc tgccttccag cgacacggga
    23341 aaacgtccgg cataacgcaa atcgttaccg accattgata attgctgagc gcgcaaactt
    23401 aactgcacgc gggcatcttt taccggctca ccatccgggt aaagcagttg cagtttgccg
    23461 ctgaccgctt cgccagtttt gaactctttt ttgtcgagag ctaaaccaat ctcgaaatgt
    23521 ggcttgatgt agtttgccac gcgaaaactg ctgctataga cctgattgcg gtaagcaaga
    23581 cgtaactcat aacctccggc tacggcattt tctggcaggc ggaaacttcc ctgcccgcca
    23641 ttgcgcgcat ccagcgtgac attgacggtt tgcaacagac tgccgttagc gtccagcacc
    23701 gaaagcttcg ccggggcgct gacgatgggg gatgaatgca acggatcgtg gaactcgcgg
    23761 cccatcactt taacatcgac acgatcgcct gcgcgatata gcgggcgatc ggtaaaaata
    23821 tacaagcggg tgttgtagat ttcgctttcg tagaagaagt tctcggagac aaaaacaccg
    23881 ccttcagcat ccttacccag aatgtatgaa cgttctggcg atatatgttg taactgcaag
    23941 gtaccgctgt catcggtcac accgcgggtc atcacgccaa gaccgtcagt ccacaagatc
    24001 tctgagccgg gcttcgcttc accctgtttt ttacccgcgg tccacaccag aagctcgttg
    24061 cctgacactt tgctaagcgc cacggtatcg gaaacaaaca ccaccgtcgt cgcccgatac
    24121 ccaccaacca tcgcctcgac gaggtacagt cccggctctt gttggccgag aggaatataa
    24181 atgttacccg gctgcggcga gatgaaattg ctggatgcgc cttccagttt taccccttgc
    24241 tgcggctcga ccggtttagc ctgccatagt ggataacgga actgttccac caagggatat
    24301 tttttcagcg gggaaaactg gttgttctgt acataacggg aaggtttaat aatggcattg
    24361 ccgagatgta attcgggtaa tgcctgagtc acattctgcc gtgactgaga agagaaagta
    24421 cgctgcatta cgcggcgaga tttgccgtac cagttatccc acagccaggt cagcgtattg
    24481 ttcagcccgt cgcccagata ttgcggttgc accacaatgc gatgcaggtt tttctgctgg
    24541 cgcaaaaatg ccatcgggtc aggaatacga tacaggcgaa cgtccacgcc gccgtactct
    24601 tccatctgat agcgccgata atcacgcccc ggcgcttcca gtcgcacttt cgcctcttca
    24661 ctgctgctaa aactgctgtc agcaagcaaa aagaatgttc ccccggcggg cggcgcatag
    24721 ttactggaag gaagcgaatc atcagcatta gcaagccctg ttcccacaag agacagggac
    24781 aagcacgctg ctatcgcgcc agaaaattta aacgataaat gccaatgaaa ttgggattgg
    24841 aatcgttggg tatccatcgg gtgtccttcc atgtcataag ttgttgcaga ctgactgcgc
    24901 gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa cgccccatcc
    24961 agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca ggcagcgcct
    25021 ggtttatgtc ctggccaata aactggctgt tgtactgaat caaattaatc gcggtcacgt
    25081 aggggccggt tttcccgttc ccctgattcc agttttgcgc cagttgacgc tgtccaggtg
    25141 ttagcgtcat ctctggcggc aaatactggc tagagaaacc gttacttttt agccatttac
    25201 tatcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca caatcctgct
    25261 gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt acaaaccagg
    25321 cgcgaaacag cccggattgt tcgacattca gcatttcgct atgggcaaca acacaacaca
    25381 gccagcaaat cagcgccagc agcccgtgcc tcatagcggc tgccaggtta tgggtagcca
    25441 ctgccaggcg gcaccgggtt ccatctgggc cagcttcatg acataacgcg gttgttgaga
    25501 taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa ccggttcgag
    25561 attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc cttgtggatt
    25621 gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg ggcggttttt
    25681 attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg agggagaaaa gcagcgtttt
    25741 gttttgcatc gccagcgaca cgcggaaaaa ataatccgac attaattgat cgggttgcgc
    25801 cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacgcc agatttgcgc
    25861 ttcgccctgc tgagcctggc ttatcggcaa aacaccttct ggcgctttgc tttcgtgcgc
    25921 accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag tgccatcaaa
    25981 ctgaccgaca aacagcgggg tttgcaattt tgagtctgca taccagcaca gccccgcggc
    26041 accgtctaac gccccattca acttgtcgtt ttcctgactg atgtgcgaaa gcatctcttc
    26101 ggcaataccg tgtgaatacg gcaccaccac acagaagctg gccccggcag gcatactgtt
    26161 ccagaccggc gtaaaatcga aactggtatc tacgctggcg gattcatcat ttaacgccac
    26221 aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag aaggcattaa
    26281 ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgctggc gtactggcgt
    26341 tttttcagca gcacgctctt ccaggccaaa gcttgcttgc cagcgttttt tgccgctcaa
    26401 caaatcacct gcgatcgccg tggcttcggt atcctgctga tcatctttaa acaacatatc
    26461 cgtgctggaa aacaccagca tcttgtcctg ataagtcgcg aacatcaggg cgttattgcc
    26521 gttatagcgc aactgataaa caggaacagt ttcactattt atcttgatgc tactgatttc
    26581 cgttttgctt aactggctgt cgctggtagc ggcaaacaac aatggctcca gcagtttgct
    26641 taaaccactg cgctggatca gcaccatata atgtgaaaga tggccttgtt tatcgtgcca
    26701 cagcgctgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt tcagcgtgag
    26761 atcgtgttca tagacaatac gacgaatgct gccttcaatg cccagacgat cggcatgatt
    26821 ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa tggtgaggag
    26881 atctttggga agctggctca gagagtcgct gtcgagaaag aggtccggct cattgagatc
    26941 gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc cactgccagc
    27001 atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac ctacaagacc
    27061 atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc ttgtgtatag
    27121 ccagccattt tttacgggca cagccaaact ttaccgtgcc ctaatacgac aaaagcccag
    27181 actttgcagc ctggactttt caattcaaac aagggagata gctccctttt ggcatgaaga
    27241 agtaaaatta ttcttcttct ggctcgtcgt caacgtccac ttccggagcg atttcatcgt
    27301 ccccttccgc ggcactgcca tcgatggtat ccagatcttc ctcgtcaacc ggttcagcaa
    27361 cacgttgcag acccactacg ttttcatctt ccgcagtacg gatgaggatc acgccctggg
    27421 tgttacggcc tacgatgctg atttccgaaa cgcgagtacg taccagcgta ccggcatcgg
    27481 tgatcatcat gatctggtcg cagtcatcta cctgtaccgc gccaaccact aaaccattac
    27541 gttcagtaac cttaatggag ataacccctt tcgtcgcacg cgacttggtt gggtattccg
    27601 ccactgcggt acgtttaccg taaccgtttt gcgttgcggt gaggattgcg ccatcgccac
    27661 gaggcacgat cagagagacg actttatcgc cttcacctaa gcgaataccg cgaacaccgg
    27721 tggtgttgca gcccatcgca cggacagaag actctttaaa gcgcaccact ttaccttcag
    27781 cggagaacag cattacttcg tcttcgccgc tggtcaggtc aacgccgatc agctcatcgc
    27841 cgtcaaccag tttgatcgcc actttaccgg cggtacgcag acggttgaac tcggtgagga
    27901 cagttttctt cacggtaccg ttagcggtcg ccatgaagac tttcacgcct tcttcaaact
    27961 cggtcactgg caggatcgca gtgatacgtt cgtcctgctc cagcggcagc aggttgacga
    28021 tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg ataaactttc atcgaataga
    28081 cgcgaccacg gctggagaag cacagaatat ggtcgtgagt gttcgccacc agcagtcggt
    28141 cgataaagtc ttcttcttta atacgtgcgg cagatttacc tttcccgcca cgacgctgcg
    28201 cttcgtattc agaaagcggc tgatacttaa cgtagccctg gtgagagagc gtcactacca
    28261 catcttcctg agtgatcaga tcttccaggt taatgtctgc gctgttggcg gtgatttcag
    28321 tacgacgttt gtcaccgaac tgttcacgaa ccagctccag ctcttcacgg atcacttcca
    28381 tcaaacgatc ggcgctacca agaatacgca acagttccgc gatctgatcc agcagctctt
    28441 tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag tttctgcaaa cgcagatcca
    28501 gaatcgcctg agcttgctgt tcggtcaggt agtacagacc atcacgcacg ccgaactctg
    28561 gctccagcca ttccggacgc gcagcatcgt cgccagcacg ttcgagcatc gcggcaacgt
    28621 tgcccagctg ccacggatta gcaaccagcg cagttttcgc ttctgcaggc gtcggcgcat
    28681 gacggatcag ttcgatgatc gggtcgatgt tcgccagcgc cacggctaat gcttcaagga
    28741 tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt acgacgggtc accacttcac
    28801 ggcggtgacg aacaaacgcc gcgatgatgt ctttcaagtt catgatcttc ggctgaccat
    28861 ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa ctgggtctgg gagtagaggt
    28921 tgttgagcac aacttcaccg accgcatcgc gtttcacttc aatcacgatg cgcataccgt
    28981 ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac gcgtttttct tttaccagtt
    29041 ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata cggaatttcg tggacgataa
    29101 tggtttcacg accggttttg gcgtcaactt ccacttctgc gcgagcgcgg atatacacct
    29161 tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg accgttaatg attgccgccg
    29221 tcgggaagtc cggccccggg atgtgttcca tcagcccttc aatgctgatg tcttcatcat
    29281 cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt gtgcggcggg atgttggttg
    29341 ccatacctac ggcgataccg gaagaaccgt tcaccagcag gttaggaatt ttggttggca
    29401 tgacgtcggg aattttttcc gtgccgtcat agttatcaac gaaatcgacc gtctcttttt
    29461 cgagatcggc catcagttca tgggcaattt tcgccagacg gatttccgta taacgcattg
    29521 ccgccgcaga gtcgccgtcg atggaaccga agttaccctg accgtctacc agcatgtaac
    29581 gcagcgagaa tggctgcgcc atacggacga tcgtgtcata aaccgccgag tcaccatggg
    29641 gatggtattt accgattacg tcaccaacga cacgggcaga ttttttatag gctttgttcc
    29701 agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg gtgtaccggc ttcaggccat
    29761 ctcggacatc tggcagcgca cggccaacaa tgaccgacat cgcataatcc agataggagc
    29821 tcttcagctc ttcctcaatg ttgaccggtg taatttctct cgcaaggtcg ctcatctaac
    29881 cgctatccct ctactgtatc ccggattcaa aggtcgcaaa ttataacaca gccgcgcagt
    29941 ttgaggtaaa cctatacgct ttattcacat ccaatgcctg atatactcgt ttgtcttgcc
    30001 aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc cggaaaacca taacgtagac
    30061 cacgaagaga tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct tgaaggtgag
    30121 ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct atattgccga gcgtgctggc
    30181 ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct ggccgagagt
    30241 atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg gctttgagcc attgcaggtg
    30301 gcaaaactgc acgcgctgga aagcggcatt caggtggatt atgtgcagga aaccgtggaa
    30361 gagcacgcgg caaaacatgc cgggcagtat gatgtggtga cctgcatgga gatgctggag
    30421 cacgtccccg atccgcagtc agtggtcaga gcctgtgcgc aactggtgaa accaggcggc
    30481 gatgtctttt tctcgacgct taaccgcaac ggcaagtcat ggctgatggc ggtggttggc
    30541 gcggaatata ttttgcgcat ggtgcccaaa ggcacgcatg atgtgaagaa gtttattaaa
    30601 ccggcagaac tgcttggctg ggtagatcag acaagcctta aagagcgaca tataaccggg
    30661 ctacattaca acccgctcac taatactttt aaactcggcc ccggcgtgga tgtgaactat
    30721 atgctgcaca cgcagaataa gtgaggttgt tgtttggccg agccaatgcc tgatgcgacg
    30781 cttgccgcgt cttatcaggc ctacaaatgc tccccgtagg ccggataagg cgtttacgcc
    30841 gcatccggca accgtgccga ctagacagtg attaccattt caccgtcatc gacaaaaacc
    30901 ctgccatctg ggcaaaatca tcactccctt tctgccacgc cacgctgccg cgcagggaca
    30961 ctcgctgact gatattgccc gtgactccca cttttatttc accccgttgc ttcaccgcat
    31021 cgtcactgat agtgctgcca tcctcttcaa tttccgtcga atggggattg tgataataat
    31081 tcagatctaa tgttggtatg acatgaacag cggtacgcca ttcgctgtgt aaacccagcc
    31141 gcatctgaat atcatcaccc tgcgattgtg acacgcgcgc acggttagcg gcggtaaaat
    31201 catcctgctg cacgccctga taaatcacct gcgcctgcgg ttcaatcacc acaccacgcc
    31261 ccggtaacca ctgatacccg gcttccagcg aggcgataat ccccgacgag tggtaatgat
    31321 ctgtgccatc gtcttgttcg gaaacatcat tgttaaacca cgcgtattgc agccagctat
    31381 ccagccaggc cccctgcttc tgattaccgt gctgatacca gcttgaggtc agcccaacgg
    31441 cataaccgtg gttctggtta tcggcgcgag ttccggtcat attcgagcgg ctctcgccct
    31501 gattatcact gtagccacca accgccccaa gcatccactc gccatcatcg ccccagcgcc
    31561 cgctaaacag gtcaccgcta agctgcaccg tagaagtgtc ttcatgctga gccagttgcc
    31621 ctgccgccgt gtaatgataa cgtccgccga taacacgtaa attcagcgtc tgaccattgc
    31681 cacctgcatg atcgcgtcgc tccatcataa acgcctgatt tgccgcccgc agattattga
    31741 gataaccgcc aactttggca ttcagcaccg gctggtaagc ggggattgga tcgggtgttg
    31801 ggtcaggatc gggtgttggg tcagggtctg gatcaggtgg caatggtggc gttacttctt
    31861 gcgatcgcag ataccagtcg ttgttatctt ccaccagcgt gtagtcatat gctcccatat
    31921 tgacgtagcc gctgcctgcc agactgaact tcgcattgtt ttgaaactgc gtgggatcgg
    31981 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
    32041 taaccaccac agttgtgttg ccagcagtat taccgttcaa caccaattga tcgcttaccg
    32101 agtcatcgcc gtttaattcg ctatcgagca gtagcgtacc gccgccggta taatcaccgt
    32161 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
    32221 tcagattgcc attaaccgtc acgtcggaaa gataggtcag gctgtcagtt tcaaggttgg
    32281 tattaagctc acctgtcaac gtcacgccat cggtggcatt caccagcgtc ccatcgccgt
    32341 taaacaatga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
    32401 catcgctgat atcgatagtg ccgctggaag tagcatcgat agactgaata tcctgatccg
    32461 cgccagtaac gaacgtcgcc ccatcaccga cccacagtga cgaagcataa ggaaagatat
    32521 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac tgccgactgc gttgtaccgc
    32581 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    32641 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    32701 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    32761 tatcgacatt ggcagtaatc tccatcacgc tttcttgttg cttatcggta aagataacct
    32821 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    32881 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    32941 catcgaccac cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac
    33001 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    33061 ggccatcatt gtatgatgtg ccgctctgcc aggtggagaa atcagagaga tcgagcacac
    33121 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggcgagatct gccgcgtcgc
    33181 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    33241 gcgcccccga cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    33301 tggtgccagc aaaactgcct tgattaacag taacattgcc accagcatcg atatttaaag
    33361 tgccattctg aaagcccgtc aatgagtgca caaaggtttg ttgggtggag ccaacgttta
    33421 gctctgcctg attctggtac ttatcaatac tccctatcgt cagaccgtag cagtcttgcg
    33481 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcgg cccagggtaa
    33541 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    33601 gatcgccgga acctgttttg gtgatcaacc cggtaccagc aatagagtca acagctccgt
    33661 cattctctgt attgccaata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    33721 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    33781 cgctgttgtt ctcatccact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    33841 tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg
    33901 cagtgttatt tgtaaaggca gtgttattta ttatcgtata acctgaagga tgcttgctga
    33961 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    34021 tatatgcctg gttgttatca aaaataacat cacttaaata gacgtcatta ttaatggtat
    34081 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    34141 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ccatagccgc
    34201 ctgcgacgtt accagaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    34261 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    34321 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggctcctc
    34381 cgctggcgtt attggtcata tccgaaaaaa ccagccattg cccatcggcg acactccagt
    34441 cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg atatcatatc
    34501 cctggcataa atcaatggcc gccgcgaaac cgttagcaga gaaaagagat gcaatcattg
    34561 acgggagtag agataaatac tccttgcgta gaaagataat ccgcatattc atctaaccat
    34621 cattttatat aagacggcat atttaaacgc attatacaat atgggttatc tttcttttgt
    34681 aaattgtcca accc
//
LOCUS       DAZFFI010000042        34494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFFI010000042 DAZFFI010000000
VERSION     DAZFFI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.042"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..434)
                     /locus_tag="ACHJ12_002354"
     CDS             complement(<1..434)
                     /locus_tag="ACHJ12_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4990130.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGG"
     gene            complement(594..806)
                     /gene="rpmE"
                     /locus_tag="ACHJ12_002355"
     CDS             complement(594..806)
                     /gene="rpmE"
                     /locus_tag="ACHJ12_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4990131.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1009..3207
                     /gene="priA"
                     /locus_tag="ACHJ12_002356"
     CDS             1009..3207
                     /gene="priA"
                     /locus_tag="ACHJ12_002356"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4990132.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3363..4388
                     /gene="cytR"
                     /locus_tag="ACHJ12_002357"
     CDS             3363..4388
                     /gene="cytR"
                     /locus_tag="ACHJ12_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4990133.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4480..5439
                     /gene="ftsN"
                     /locus_tag="ACHJ12_002358"
     CDS             4480..5439
                     /gene="ftsN"
                     /locus_tag="ACHJ12_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4990134.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5532..6062
                     /gene="hslV"
                     /locus_tag="ACHJ12_002359"
     CDS             5532..6062
                     /gene="hslV"
                     /locus_tag="ACHJ12_002359"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4990135.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6072..7403
                     /gene="hslU"
                     /locus_tag="ACHJ12_002360"
     CDS             6072..7403
                     /gene="hslU"
                     /locus_tag="ACHJ12_002360"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4990136.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7470..8396
                     /gene="menA"
                     /locus_tag="ACHJ12_002361"
     CDS             7470..8396
                     /gene="menA"
                     /locus_tag="ACHJ12_002361"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4990137.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8489..8974
                     /gene="rraA"
                     /locus_tag="ACHJ12_002362"
     CDS             8489..8974
                     /gene="rraA"
                     /locus_tag="ACHJ12_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4990138.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9059..9304)
                     /gene="zapB"
                     /locus_tag="ACHJ12_002363"
     CDS             complement(9059..9304)
                     /gene="zapB"
                     /locus_tag="ACHJ12_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4990139.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9730..10575
                     /gene="glpF"
                     /locus_tag="ACHJ12_002364"
     CDS             9730..10575
                     /gene="glpF"
                     /locus_tag="ACHJ12_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4990140.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10598..12106
                     /gene="glpK"
                     /locus_tag="ACHJ12_002365"
     CDS             10598..12106
                     /gene="glpK"
                     /locus_tag="ACHJ12_002365"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4990141.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12241..13251
                     /gene="glpX"
                     /locus_tag="ACHJ12_002366"
     CDS             12241..13251
                     /gene="glpX"
                     /locus_tag="ACHJ12_002366"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4990142.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13348..14094
                     /gene="fpr"
                     /locus_tag="ACHJ12_002367"
     CDS             13348..14094
                     /gene="fpr"
                     /locus_tag="ACHJ12_002367"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4990143.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14099..14527)
                     /gene="uspD"
                     /locus_tag="ACHJ12_002368"
     CDS             complement(14099..14527)
                     /gene="uspD"
                     /locus_tag="ACHJ12_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4990144.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14554..14853)
                     /gene="yiiS"
                     /locus_tag="ACHJ12_002369"
     CDS             complement(14554..14853)
                     /gene="yiiS"
                     /locus_tag="ACHJ12_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4990145.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15065..15505)
                     /gene="yiiR"
                     /locus_tag="ACHJ12_002370"
     CDS             complement(15065..15505)
                     /gene="yiiR"
                     /locus_tag="ACHJ12_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4990146.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15606..16205
                     /gene="yiiQ"
                     /locus_tag="ACHJ12_002371"
     CDS             15606..16205
                     /gene="yiiQ"
                     /locus_tag="ACHJ12_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4990147.1"
                     /translation="MKPGCTLFFLLYSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16313..17080
                     /gene="tpiA"
                     /locus_tag="ACHJ12_002372"
     CDS             16313..17080
                     /gene="tpiA"
                     /locus_tag="ACHJ12_002372"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4990148.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17135..17890)
                     /gene="cdh"
                     /locus_tag="ACHJ12_002373"
     CDS             complement(17135..17890)
                     /gene="cdh"
                     /locus_tag="ACHJ12_002373"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4990149.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17997..18986)
                     /gene="sbp"
                     /locus_tag="ACHJ12_002374"
     CDS             complement(17997..18986)
                     /gene="sbp"
                     /locus_tag="ACHJ12_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4990150.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19306..20268)
                     /gene="pfkA"
                     /locus_tag="ACHJ12_002375"
     CDS             complement(19306..20268)
                     /gene="pfkA"
                     /locus_tag="ACHJ12_002375"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4990151.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20449..21351)
                     /gene="fieF"
                     /locus_tag="ACHJ12_002376"
     CDS             complement(20449..21351)
                     /gene="fieF"
                     /locus_tag="ACHJ12_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4990152.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21500..22000)
                     /gene="cpxP"
                     /locus_tag="ACHJ12_002377"
     CDS             complement(21500..22000)
                     /gene="cpxP"
                     /locus_tag="ACHJ12_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4990153.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22150..22848
                     /gene="cpxR"
                     /locus_tag="ACHJ12_002378"
     CDS             22150..22848
                     /gene="cpxR"
                     /locus_tag="ACHJ12_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4990154.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22845..24218
                     /gene="cpxA"
                     /locus_tag="ACHJ12_002379"
     CDS             22845..24218
                     /gene="cpxA"
                     /locus_tag="ACHJ12_002379"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4990155.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRRGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24324..24998)
                     /gene="yiiM"
                     /locus_tag="ACHJ12_002380"
     CDS             complement(24324..24998)
                     /gene="yiiM"
                     /locus_tag="ACHJ12_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4990156.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25147..26130)
                     /gene="kdgT"
                     /locus_tag="ACHJ12_002381"
     CDS             complement(25147..26130)
                     /gene="kdgT"
                     /locus_tag="ACHJ12_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4990157.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPIITSIWSRKVKARAAKIEILGTVK"
     gene            complement(26390..27010)
                     /gene="sodA"
                     /locus_tag="ACHJ12_002382"
     CDS             complement(26390..27010)
                     /gene="sodA"
                     /locus_tag="ACHJ12_002382"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4990158.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27295..28329
                     /gene="rhaT"
                     /locus_tag="ACHJ12_002383"
     CDS             27295..28329
                     /gene="rhaT"
                     /locus_tag="ACHJ12_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO4990159.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28326..29174)
                     /gene="rhaR"
                     /locus_tag="ACHJ12_002384"
     CDS             complement(28326..29174)
                     /gene="rhaR"
                     /locus_tag="ACHJ12_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4990160.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29248..30084)
                     /gene="rhaS"
                     /locus_tag="ACHJ12_002385"
     CDS             complement(29248..30084)
                     /gene="rhaS"
                     /locus_tag="ACHJ12_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO4990161.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30372..31841
                     /gene="rhaB"
                     /locus_tag="ACHJ12_002386"
     CDS             30372..31841
                     /gene="rhaB"
                     /locus_tag="ACHJ12_002386"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO4990162.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31838..33097
                     /gene="rhaA"
                     /locus_tag="ACHJ12_002387"
     CDS             31838..33097
                     /gene="rhaA"
                     /locus_tag="ACHJ12_002387"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4990163.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLSWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33339..34163
                     /gene="rhaD"
                     /locus_tag="ACHJ12_002388"
     CDS             33339..34163
                     /gene="rhaD"
                     /locus_tag="ACHJ12_002388"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4990164.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34173..34487
                     /gene="rhaM"
                     /locus_tag="ACHJ12_002389"
     CDS             34173..34487
                     /gene="rhaM"
                     /locus_tag="ACHJ12_002389"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4990165.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8421 a         9013 c         8983 g         8077 t
ORIGIN      
        1 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
       61 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      121 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      181 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      241 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      301 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc gatgcgcacc
      361 ccggctgaac cctggacaat gctaccgcca tactgcgtca tgtcgccctg acgcgccgcc
      421 ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc ctagcgccag
      481 tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc
      541 aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
      601 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
      661 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
      721 ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg
      781 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
      841 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
      901 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
      961 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc
     1021 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
     1081 acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg
     1141 gttgtggtgt cagttagcga tgtcagtgag ctgccgctta acgagctgaa agcggtagtg
     1201 gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcggttact gctctgggcg
     1261 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg
     1321 cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggc
     1381 caggcggtgg atctgaacag cctgaaacgc tcccccaagc aacaacaggc gctggcggcg
     1441 ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc tcgaatttaa tgatgccgcg
     1501 ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
     1561 gactggcaaa cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc
     1621 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
     1681 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
     1741 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
     1801 tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt ccggcctgaa cgacagcgag
     1861 cggctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc
     1921 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgatga agagcacgac
     1981 agctcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt
     2041 gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgttg
     2101 tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc ggggaatgcg
     2161 cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc aggtctggct
     2221 ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt
     2281 cttaatcgtc ggggttttgc gcctgcgcta ctgtgccacg actgcggctg gattgccgaa
     2341 tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac
     2401 cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg
     2461 gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct gttccctgat
     2521 gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa
     2581 ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat gctggcgaaa
     2641 ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt
     2701 tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt cgctggtcgt
     2761 gccgggcgtg cgggtaaaca aggcgaagtg gtgctgcaaa cgcaccatcc agaacatcct
     2821 ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc gctggctgag
     2881 cgacggatga tgcaactgcc gccatggacc agccatgtga ttgtgcgtgc ggaagatcat
     2941 aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct ctccagcccg
     3001 ctggcagacg acaaactgtg ggttctcggt ccggttccgg cactggcacc taaacgtggc
     3061 ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt gcaacacatc
     3121 attagtggta cgctggcgct catcaataca ataccggatt cccgcaaggt gaaatgggta
     3181 ctggatgttg atccgattga gggttaaacc gcttacgatg cgaggcggat cgaaaaattc
     3241 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
     3301 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
     3361 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
     3421 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
     3481 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
     3541 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc
     3601 ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg
     3661 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
     3721 tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca
     3781 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca ccggaactgg
     3841 agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta aattatctat
     3901 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc
     3961 actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc
     4021 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg cagcagctgc
     4081 ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg
     4141 cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt
     4201 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc
     4261 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
     4321 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
     4381 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
     4441 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
     4501 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
     4561 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
     4621 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
     4681 cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa
     4741 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa
     4801 gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct
     4861 gatatgcgcc agcagccaac gcagctggtt gaggttccgt ggaatgaaca gacgccggaa
     4921 cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga gcagcaacgc
     4981 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcacaa
     5041 gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac
     5101 caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
     5161 ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
     5221 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
     5281 ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc
     5341 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg
     5401 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
     5461 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
     5521 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
     5581 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
     5641 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
     5701 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg
     5761 ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
     5821 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
     5881 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgtgcac
     5941 tgttagaaaa tactgaactg agtgctcgtg aaattgctga aaaggcgttg gatattgcag
     6001 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
     6061 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
     6121 tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc
     6181 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
     6241 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
     6301 cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctacgtcggt aaggaagtgg
     6361 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
     6421 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc
     6481 cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc
     6541 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc
     6601 ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca
     6661 gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga
     6721 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
     6781 aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
     6841 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
     6901 ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg
     6961 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
     7021 acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag gcgctgacca
     7081 ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
     7141 cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt aagcgcatcg
     7201 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca
     7261 ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
     7321 ctatcatcat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
     7381 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
     7441 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
     7501 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
     7561 ggacagcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactga
     7621 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa
     7681 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
     7741 cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc
     7801 tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg
     7861 gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata
     7921 tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagtgtc atggggagct
     7981 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc gcatgcggcc
     8041 tgctggcaac ggcagtactg aatattaata acctgcgtga tatcaatagc gaccgcgaaa
     8101 atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct
     8161 gcctgctgat gggctcactg gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc
     8221 tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga
     8281 tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt
     8341 tactgactaa cctgctgttt attttaggga tattcctaag ccagtgggca gcataactga
     8401 caaatatcaa ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa
     8461 attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat
     8521 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
     8581 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
     8641 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg
     8701 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt
     8761 catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
     8821 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
     8881 caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
     8941 tattctttca gaagatccgc tggatattga atgatagaaa aggcaccgca aggtgccttt
     9001 tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg aagcgtaatc
     9061 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagcca ttctgctgtt
     9121 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
     9181 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
     9241 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
     9301 tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc cgattaccgg
     9361 tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa gccgcgccat
     9421 cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc cttctcctgg
     9481 tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa
     9541 catgcatcat gtacaatcag atggaataaa tggcgcgata acgctcattt tatgacgaag
     9601 cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag tttacagcat
     9661 gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt aactcttcag
     9721 gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga attcctcggt
     9781 accgggttgt tgattttctt cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg
     9841 tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat ggccatctac
     9901 ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc attgtggctg
     9961 tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt tgccggcgct
    10021 ttctgtgctg cggctttagt ttacgggctt tactacaatt tatttttcga cttcgagcag
    10081 actcatcaca ttgttcgcgg cagcgttgaa agtgttgatc tggctggcac tttctctact
    10141 taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt gattaccgct
    10201 attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc acgcggccct
    10261 ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat gggcccattg
    10321 acaggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc ctggctggcg
    10381 ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct ggtgccgctt
    10441 ttcggcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact gattggtcgc
    10501 catttgcctt gcgatatctg tgttgtggaa gaaaaggaaa ccacaactcc ttcagaacaa
    10561 aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa aatatatcgt
    10621 tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg atgccaatat
    10681 cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggtt gggtagaaca
    10741 cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc
    10801 cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg aaaccactat
    10861 tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc agtgccgtcg
    10921 taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata tccgcagcaa
    10981 taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca
    11041 tgtggaaggc tctcgcgagc gtgcacgtcg tggtgaattg ctgtttggta cggttgatac
    11101 gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca ccaacgcctc
    11161 tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc tggaagtgct
    11221 ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtat acggtcagac
    11281 taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg gtgaccagca
    11341 ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac
    11401 tggctgcttt atgctgatga acactggcga gaaagcggtg aaatcagaaa acggcctgct
    11461 gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgttggaag gtgcggtgtt
    11521 tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta acgacgccta
    11581 cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc
    11641 atttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga ttttcggtct
    11701 gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta ttgcttatca
    11761 gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc acgccctgcg
    11821 cgtggatggt ggcgcagtag caaacaattt cctgatgcag ttccagtccg atattctcgg
    11881 cacccgcgtt gagcgcccgg aagtgcgcga agtcaccgca ttgggtgcgg cctatctcgc
    11941 aggcctggcg gttggcttct ggcagaacct cgacgagctg caagagaaag cggtgattga
    12001 gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg caggctggaa
    12061 aaaagcggtt aaacgcgcga tggcgtggga agaacacgac gaataatgta aatgccgaat
    12121 gaagcgttta tgccgcatcc ggtagtcccg aaacgtgcgg gggcaacccc gcacacatca
    12181 ataatccctc ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct
    12241 atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatcagcggc gctggctggc
    12301 tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg
    12361 cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc
    12421 gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta
    12481 gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg
    12541 ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg
    12601 gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg
    12661 gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg
    12721 atactggcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc
    12781 gtatttgcta ttccggatgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc
    12841 gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtagtttc tgcggcggtg
    12901 atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc
    12961 gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc
    13021 atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa cgtcatcttc
    13081 tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa aggcaatatc
    13141 gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc
    13201 atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg attgatttga
    13261 tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag
    13321 gcaagttaat caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa
    13381 gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt
    13441 accgccgggc aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc
    13501 tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc
    13561 gatggcaaat taagcccacg attggcggca ctgaaaccag gcgatgaagt gcaggtggtt
    13621 agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg
    13681 ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta
    13741 gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat
    13801 ttgccactga tgcaagaact ggaaaaacgc tacgaaggga aactgcgcat tcagacggtg
    13861 gtcagtcggg aaacggcagc ggggtcgctc accgggcgga taccggcgtt aattgaaagt
    13921 ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg
    13981 tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg
    14041 acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt
    14101 acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat
    14161 atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact
    14221 gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt
    14281 ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct
    14341 tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta
    14401 agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccagag
    14461 ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat
    14521 aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc
    14581 aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc
    14641 ctccagatcc ttaatcttgt aggccacgct ggccccttca tctgcagagg atgcagcggc
    14701 ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg
    14761 cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc
    14821 tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct
    14881 gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta
    14941 tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggca
    15001 ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta
    15061 aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgggta tctttaccat
    15121 atttattttc gccttgtgtg ccaatgaacg ctccaagatc gataagcatc atcaccagaa
    15181 tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgcccagt
    15241 tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc
    15301 gatcatgcaa gcgcttaacg gttactgccg ctgttggcca aagcaagcac acaaggcaaa
    15361 acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc accagcatgc
    15421 ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg
    15481 agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct
    15541 acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac
    15601 tgtcaatgaa gccagggtgt acgctgtttt ttctcttata ttctgcatta accgttacaa
    15661 caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag
    15721 cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca
    15781 gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc
    15841 gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct
    15901 taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag
    15961 cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga
    16021 ccaaaacaca aagccagaaa aaactgcagt cgctgctaac ggcggggaaa aacaaacgtt
    16081 attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc
    16141 tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata
    16201 aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg
    16261 ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca
    16321 tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt
    16381 ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc
    16441 ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc
    16501 gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct
    16561 gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa
    16621 agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc
    16681 ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg
    16741 cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt
    16801 tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc
    16861 acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga
    16921 acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc
    16981 tcagccagat atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc
    17041 agtaatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt
    17101 catatccggc acttactttc cttaactctt cgccttaacg caaaatctca cactgatgat
    17161 cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt gtcgccagca
    17221 atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc
    17281 gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc agttcgcttt
    17341 ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc
    17401 ggctgctgat gttcgccaga ttgttatcca gttgttcgcg cacatcagga cgaatacaag
    17461 agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa
    17521 ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg
    17581 ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa
    17641 tacgatacgt tggcatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt
    17701 atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg
    17761 gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc
    17821 agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg
    17881 cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga
    17941 caaataaaaa acccggtcac gagaaaccac cgggtttgat aattatcctg gggaaatcag
    18001 cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc
    18061 cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac
    18121 tttttcgcca cctcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct
    18181 tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg
    18241 accactttat cgaccaccga cacggttggc tctgcgagga tagactcact cggcgtgacg
    18301 atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg
    18361 atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa
    18421 tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg
    18481 ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca
    18541 gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca
    18601 tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg
    18661 gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg
    18721 tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac
    18781 gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc
    18841 tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca
    18901 tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat
    18961 gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca
    19021 aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg
    19081 ggtatatgtt gggggttaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    19141 ctttatcgat gccggatgcg gcgcgaacgc cttatccggc ctacaaaaat ttgtaaattc
    19201 aataaattgc agaattcacg tagatactga taagcgaagc gcatcaggca tttttgcttc
    19261 tgtcatcggt ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt
    19321 cgcgcagtcc agccagtcgc ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat
    19381 gtcgtggtga accagctgtt cgttctggat acctacgcaa cgaccgccgt aacctgccag
    19441 cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg
    19501 agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt
    19561 ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc
    19621 gtgtttttta cctttcgcga tacccgcttt gatttcgttt accagatctt cacggctgaa
    19681 ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag ccaacgtcag
    19741 atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt
    19801 gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt
    19861 gtagtcagtg cctttgatgt cgttgtcgat agtgccaggc agaccgatgc acgggaagcc
    19921 catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc
    19981 gtcgatacca cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa
    20041 ttccgggaaa cgcgcagaac cgaggaacgt accaccacgg ttgatcatgt cagaaacgct
    20101 gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt cataaatgcc
    20161 cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat
    20221 gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga ctacctctga
    20281 actttggaat gcaaaataaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca
    20341 acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat
    20401 ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat
    20461 agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa ttacatccga
    20521 tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac
    20581 caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg
    20641 ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat
    20701 ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc
    20761 catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc
    20821 gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt tcatcataac
    20881 atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac
    20941 ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac
    21001 cccgacgcct ggatctgtca tcggtgttgg agatatcaga tgttgaatac ccgtcaaaaa
    21061 caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactctgc
    21121 tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag
    21181 taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact
    21241 caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc
    21301 cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc
    21361 cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg
    21421 caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat ggtgtctatg
    21481 gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat agcttcaacg
    21541 atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt tgatgtttct
    21601 cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg cggacttttg
    21661 ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt gcgcgcacag
    21721 cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat
    21781 taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc tgacgctgat
    21841 gttcggttaa acttatgccg tcgaacatat ggctctgcgt actgcgctgc gtaagttctt
    21901 cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat gaactgactg
    21961 ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc caaaatcttt
    22021 ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg tcagggggtg
    22081 taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg cctcggaggt
    22141 atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac ttccctatta
    22201 aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg ggaacaggcg
    22261 cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat gccgaagaaa
    22321 aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt cattatgttg
    22381 acggcgcgcg gcagcgaact tgatcgcgtt ctcggccttg agctgggcgc agatgactat
    22441 ctcccgaaac catttaatga tcgtgagctg gtggcacgta ttcgcgcgat cctgcgccgt
    22501 tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact ggaagttgat
    22561 gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac gctggagtta
    22621 accggtactg agtttaccct gctctatttg ctggcacagc atctgggtca ggtggtttcc
    22681 cgtgaacatt taagccagga agtgttgggc aaacgcctga cgcctttcga ccgcgctatt
    22741 gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg
    22801 tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata ggcagcttaa
    22861 ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg ttggttttga
    22921 tgttacccaa gctcgattca cgccagatga ccgagcttct ggatagcgaa cagcgtcagg
    22981 ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc aacgatttaa
    23041 tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc accgccagga cagcgtttgt
    23101 tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc
    23161 gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag tatggccgcg
    23221 tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa ctttatctga
    23281 ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac cgcccgctat
    23341 tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg gcctggagtc
    23401 tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag ggaaacttac
    23461 gccagcaccc ggaactggaa gcggggccac aggaattcct tgccgctggt gccagtttta
    23521 accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg ctttctgata
    23581 tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc
    23641 gtcgtagagg tgaaagcaag gaactggagc gtattgaaac cgaagcgcaa cgtctggaca
    23701 gcatgatcaa cgatctgttg gtgatgtccc gtaatcagca aaaaaacgcg ctggttagcg
    23761 aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg ttcgaagccg
    23821 agcagatggg caagtcgttg acggttaact tcccgcctgg gccgtggccg ctgtacggca
    23881 atccgaacgc cctggaaagt gcgctggaaa acattgttcg taatgctctg cgttattccc
    23941 atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt acggtggacg
    24001 acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg ttctatcgga
    24061 ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggcg attgttgaaa
    24121 ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg ggcggtttac
    24181 ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg taggcaggat
    24241 aaggcgttta cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc
    24301 ttatcaggcc tacactccgg gggttatttt ccccacaacc ggcgggaaaa atcttcgatc
    24361 ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg
    24421 gagagtaaac ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc
    24481 tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcgtc cgcagacact
    24541 ttccctggcg ctatcacgct atacaaccac cccaccttac ctgtgttttg catcaactgc
    24601 gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggcgagcg cggctggctg
    24661 acctgaatta atgcctctcc ccagcggaaa atatcgccca tatgaacatt actttccgtg
    24721 agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc
    24781 ggaaattccc gcacccagta gagatagtgc tcacgaggat aatgacacag cgctctgtct
    24841 ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc
    24901 atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc
    24961 tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat
    25021 ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt aagtatccag
    25081 gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa
    25141 gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg
    25201 tgaccagata gaggtaataa ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac
    25261 cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac
    25321 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
    25381 accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa ttactgccac
    25441 acccaacagg atccccagca aaccagtctg ggcaattaca gtcaaatcaa tggtattgcc
    25501 cagcgcgaag gcaaagaatg gaatcagcgt ctgcaccgct ttgctgaaaa attctcgtaa
    25561 ttcagggtca aggttcccca gggcaaagcc caccaggaac ggtaatactg cgccgacgaa
    25621 aacatgcggt tcaaacgagg caatccctgc agtgcccaga ataatcatcg tcatgagcgg
    25681 cccggactcc aacgacatca acacaaatgc cccagcttct tcttttgtgc cgtactgctg
    25741 catgatggaa gcgtaaagtc cgccattggt catatccatc gccgccacca gcgccagcgt
    25801 tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat
    25861 tgccgcaacc acccacgcga cggcaatttt tgttaccacc agcgtaccgg atttacgcaa
    25921 taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag
    25981 aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc
    26041 cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg
    26101 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    26161 ctgatgcgtc atttctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt
    26221 taatcaataa aagtgatcaa aaccacatca attgaaacac tgttttattt ttatcggatc
    26281 attgttttta ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt
    26341 ttaagctgat atgcggcgta aacgcctcat tgcagctggc ggcaaatgat tattttttcg
    26401 ccgcgaaacg cgccgcagct tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt
    26461 ccgggcggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa
    26521 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc tggttagcag
    26581 tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc
    26641 gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    26701 cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca
    26761 ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca
    26821 gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt
    26881 ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg tggatttcca
    26941 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    27001 tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag
    27061 ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt
    27121 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
    27181 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
    27241 ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa gaagatgagt
    27301 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
    27361 tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg
    27421 attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc ggatttctgg
    27481 gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
    27541 tgggggatcg gtaatatcaa ctacggcctg accatgcgtt atctcggcat gtcgatggga
    27601 attggcatcg ccattggcat tacgttgatt gtcggtacac tgatgacgcc aattatcaac
    27661 ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttgct cggcgttctg
    27721 gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga gcgcaaaatg
    27781 ggcattaaag ccgaagagtt caacctgaaa aaagggctgg tgctggcggt gatgtgcggc
    27841 attttctctg ccggtatgtc ttttgcgatg aacgccgcaa aaccgatgca tgaagccgct
    27901 gccgcactgg gcgtcgatcc actgtatgtc gctctgccaa gctatgtcat catcatgggc
    27961 ggcggcgcga tcatcaacct cggtttctgc ttcattcgtc tggcaaaagt gaaggatttg
    28021 tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctca
    28081 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcccgc
    28141 attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc
    28201 ggcggtatcg tcgggctggt gctgaaagag tggaacaatg caggtcgccg tccggtaacg
    28261 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcca acatcgtcgg catcggcatg
    28321 gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc
    28381 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca
    28441 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgccgc
    28501 agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
    28561 ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc
    28621 agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg
    28681 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg
    28741 ttagcaaacg acacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc
    28801 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc
    28861 ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
    28921 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag
    28981 agatcgccac gggtaatgcg ataagggcga tcgttgagta catgcagacc attaccgcgc
    29041 cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaaaacatc ttgcggataa
    29101 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
    29161 aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
    29221 cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa ccatcccgtc cctgacgaat
    29281 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
    29341 gaatccacag cgataggcga tatcagtaac gctggcctcg ctgtggcgta gtagatgtcg
    29401 ggctttcatc agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg tttgctgctt
    29461 aagctgccga tgtagcgtac gcagcgaaag agaaaattgc tccgccacgg catcccaatt
    29521 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
    29581 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa
    29641 gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
    29701 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtggg aaggatactg
    29761 cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
    29821 cgagcgatac agcacattag tcagacacag attatcggta tgttcataca gatgccgatc
    29881 atggtcgcgc acaaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac
    29941 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
    30001 aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa
    30061 atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
    30121 aatcacgagg tcaggttctt accttaaatt ttcgacggga aaccacgtaa aaaacgtcga
    30181 tttttcaaga tacagcatga attttcagga aatgcggtga gcatcacacc accacaattc
    30241 agcaatttgt gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg
    30301 tcagtagcga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaaattcagc
    30361 aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc
    30421 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
    30481 gttttaaaaa tgggctgcat agccagaacg gttatgtcac ctggaatgtg gatagcctgg
    30541 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
    30601 ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg
    30661 gcctgcccgt tgcttatcgc gatagccgct ccaatggcct aatggcgcag gcacagcaac
    30721 aactcggcaa acgcgatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacgc
    30781 tttatcaact gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg
    30841 ctctgctgat gccggattac ttcagctatc gcctgaccgg caagatgaac tgggagtaca
    30901 ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
    30961 tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
    31021 ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
    31081 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt
    31141 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
    31201 tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata
    31261 ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac gatctcccgg
    31321 cgcttatcgc cgcgacacag gcacttccgg cctgtcgctt catcatcaat cccaatgacg
    31381 atcgctttat taatcctgaa gcgatgtgca gcgaaattca ggctgcgtgt cgggaaacgg
    31441 cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgc
    31501 tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac
    31561 tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc gccgatgcct
    31621 gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat atcggcatcc
    31681 agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca
    31741 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
    31801 agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
    31861 ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg
    31921 ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt
    31981 tgaaaacccg gaaggttcgc tgaccggtgg gattcaagct actggtaatt atccgggcaa
    32041 agcgcgtaat gccagtgagc tacgtaccga tctggaacag gctatgcggc tgattccggg
    32101 gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga
    32161 tcagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
    32221 tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctttc
    32281 ccatgccgac gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt
    32341 ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga
    32401 tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact
    32461 ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa
    32521 attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta
    32581 tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc cgactgaagt
    32641 gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag
    32701 ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat
    32761 tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt
    32821 cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
    32881 cctgctgcgt gcgttgctgg aacctaccgc cgagctgcgc aagttggaag cggcgggcga
    32941 ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgtcgtggc aggcggtctg
    33001 ggaaatgtat tgccaacgtc acgatacccc aacgggtagc gaatggctgg agagcgtgcg
    33061 ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacgagc
    33121 gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct
    33181 ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacactgctc
    33241 cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat
    33301 gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct
    33361 ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc tgggacgagc
    33421 gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgcct tataaagaca
    33481 acttccatgc acaaccgcgc tatatcccgc tcagccagcc catgccttta ctggcaaata
    33541 caccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt gatcctgcag
    33601 ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt ctctgggggt
    33661 taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc
    33721 gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga
    33781 tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag
    33841 gcagcaccga gtgtctggtg gtattcccgg atggcgttgg cattctgccg tggatggtgc
    33901 ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt
    33961 tgtggccctt ccacggcgtc ttcggcagcg ggtcgacgtt ggatgaaaca ttcggtttaa
    34021 tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga
    34081 aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac
    34141 tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg
    34201 ttaaccccga cgcccacgaa gagtatcagc gtcggcataa ccccatctgg ccggaactgg
    34261 aagcagtgtt gaaatctcac ggtgcgcata actacgccat ttacctcgac aaagcgcgta
    34321 atctgctgtt tgccacggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta
    34381 ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccggata
    34441 acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg ccca
//
LOCUS       DAZFFI010000043        33790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFFI010000043 DAZFFI010000000
VERSION     DAZFFI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.043"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..543)
                     /locus_tag="ACHJ12_002390"
     CDS             complement(<1..543)
                     /locus_tag="ACHJ12_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4990166.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSAGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADYCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
     gene            complement(1569..2504)
                     /locus_tag="ACHJ12_002391"
     CDS             complement(1569..2504)
                     /locus_tag="ACHJ12_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO4990167.1"
                     /translation="MVFQESNNRDVARKYAEYITSVPLRRFVAEKVREYCGDLPLSIF
                     DGACGSGQLEQFLTVGSLYAVDIQASPLLHIPDNFPDATVKTVNENFFNAIDDVSDTD
                     TFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGVLDDAFYVLASMKARFSFFLC
                     FPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSVLFVIIDNEKQSDDVVVYGVG
                     FNGETCKKSAPVTEKINTDNWNRPYIAEEKEVIDIVQVTAELLVRQTRRRKLEDEHNA
                     FVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            complement(2590..2666)
                     /locus_tag="ACHJ12_002392"
     tRNA            complement(2590..2666)
                     /locus_tag="ACHJ12_002392"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Arg,seq:tct)
     gene            complement(2680..2756)
                     /locus_tag="ACHJ12_002393"
     tRNA            complement(2680..2756)
                     /locus_tag="ACHJ12_002393"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2720..2722),aa:Arg,seq:tcg)
     gene            complement(2764..2839)
                     /locus_tag="ACHJ12_002394"
     tRNA            complement(2764..2839)
                     /locus_tag="ACHJ12_002394"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2804..2806),aa:Ile,seq:cat)
     gene            complement(2880..3938)
                     /locus_tag="ACHJ12_002395"
     CDS             complement(2880..3938)
                     /locus_tag="ACHJ12_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4990168.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRHYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(4089..4286)
                     /locus_tag="ACHJ12_002396"
     CDS             complement(4089..4286)
                     /locus_tag="ACHJ12_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4990169.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4306..4503)
                     /locus_tag="ACHJ12_002397"
     CDS             complement(4306..4503)
                     /locus_tag="ACHJ12_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990170.1"
                     /translation="MMTSLLPLCKRHLNGAFVVFIKRSPPLAGFFMPEKRHRALNALV
                     VANTGLFSLLAFLTRVIGVSR"
     gene            complement(4629..5030)
                     /locus_tag="ACHJ12_002398"
     CDS             complement(4629..5030)
                     /locus_tag="ACHJ12_002398"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4990171.1"
                     /translation="MVLKYQPSVRSVLMCDFRGMVVPEIVKVRPVVVVSRNRHNNQLV
                     TVVPISTTEPIPRRDCHHELSENPIPGNEHITCWVKCDMLMTVSLSRLDRIKTRTWEG
                     RNYIVPMIAEDEFENIKRAVLHGIGMAYLYR"
     gene            complement(5224..5913)
                     /locus_tag="ACHJ12_002399"
     CDS             complement(5224..5913)
                     /locus_tag="ACHJ12_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4990172.1"
                     /translation="MNNQYLQFVREQLMIATADLSVSIKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIALVSPVEFSTSSWRRAVLELEAHQK
                     AWLLWCYGGNICWEYQIAITQWAWNEFNTQSGTRKIAGKTLERLKKLIWLAAQAVKAE
                     LFGGEGYEYRDLASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQNIAKLD"
     gene            complement(5906..6268)
                     /locus_tag="ACHJ12_002400"
     CDS             complement(5906..6268)
                     /locus_tag="ACHJ12_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4990173.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            complement(6281..7330)
                     /locus_tag="ACHJ12_002401"
     CDS             complement(6281..7330)
                     /locus_tag="ACHJ12_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4990174.1"
                     /translation="MRVLLRPVLVPELGLVVIKPGRELLPVFHRGRVLVEPEPKSMRS
                     LPSGVVPVVCQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            complement(7332..7604)
                     /locus_tag="ACHJ12_002402"
     CDS             complement(7332..7604)
                     /locus_tag="ACHJ12_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990175.1"
                     /translation="MTQHIKSHNSEADPEIKRGWRFRTPQYGWFHYLFCTTDEADMLQ
                     EAYLRRGVRVERSLNVDRLTWTVSVYLPVRAHLPRTNACYRQRVWR"
     gene            complement(7727..8071)
                     /locus_tag="ACHJ12_002403"
     CDS             complement(7727..8071)
                     /locus_tag="ACHJ12_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4990176.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIIKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(8193..8348)
                     /locus_tag="ACHJ12_002404"
     CDS             complement(8193..8348)
                     /locus_tag="ACHJ12_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4990177.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(8639..8992)
                     /locus_tag="ACHJ12_002405"
     CDS             complement(8639..8992)
                     /locus_tag="ACHJ12_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4990178.1"
                     /translation="MIDRTKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMCKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(9007..9357)
                     /locus_tag="ACHJ12_002406"
                     /pseudo
     CDS             complement(9007..9357)
                     /locus_tag="ACHJ12_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9359..9703)
                     /locus_tag="ACHJ12_002407"
     CDS             complement(9359..9703)
                     /locus_tag="ACHJ12_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4990179.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     CEIRHREVDIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(9690..9995)
                     /locus_tag="ACHJ12_002408"
     CDS             complement(9690..9995)
                     /locus_tag="ACHJ12_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4990180.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKREGHTVLNPSVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEWD"
     gene            complement(9992..10273)
                     /locus_tag="ACHJ12_002409"
     CDS             complement(9992..10273)
                     /locus_tag="ACHJ12_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4990181.1"
                     /translation="MILRQCAGTIKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     QSAKYSQRDIEQARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVQA"
     gene            complement(10306..11022)
                     /locus_tag="ACHJ12_002410"
     CDS             complement(10306..11022)
                     /locus_tag="ACHJ12_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4990182.1"
                     /translation="METVFDALKALKKASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVKETT
                     TQEEIKTESVAVTVQSQPSFTRKHQDGLILPSLHVANRELRRAKGQVQKWERVCAALW
                     ELNKCRDILRDITATREQQR"
     gene            complement(11056..11517)
                     /locus_tag="ACHJ12_002411"
     CDS             complement(11056..11517)
                     /locus_tag="ACHJ12_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4990183.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWTKHVGNGMSVPPVRRQLEGAKHPKGPTPIEWLKQEYERRKAAGFI"
     gene            complement(11510..12520)
                     /locus_tag="ACHJ12_002412"
     CDS             complement(11510..12520)
                     /locus_tag="ACHJ12_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO4990184.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVHE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            complement(12607..13044)
                     /locus_tag="ACHJ12_002413"
     CDS             complement(12607..13044)
                     /locus_tag="ACHJ12_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4990185.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDSGSSGESVVVH"
     gene            complement(13028..13309)
                     /locus_tag="ACHJ12_002414"
     CDS             complement(13028..13309)
                     /locus_tag="ACHJ12_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO4990186.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTQTLTQPLSRSLIHENQA"
     gene            13411..13830
                     /locus_tag="ACHJ12_002415"
     CDS             13411..13830
                     /locus_tag="ACHJ12_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990187.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTDTTSNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEISKKKARNAS"
     gene            14096..14248
                     /locus_tag="ACHJ12_002416"
     CDS             14096..14248
                     /locus_tag="ACHJ12_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4990188.1"
                     /translation="MDTLNLGNNESLVCGVFPNLDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTD"
     gene            14404..14583
                     /locus_tag="ACHJ12_002417"
                     /pseudo
     CDS             14404..14583
                     /locus_tag="ACHJ12_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15040..15168)
                     /locus_tag="ACHJ12_002418"
     CDS             complement(15040..15168)
                     /locus_tag="ACHJ12_002418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990189.1"
                     /translation="MEEAPAILTAAPFRGLPQPEAHDRIKFNDKPYILAVRFRVMP"
     gene            15455..15643
                     /gene="dicB"
                     /locus_tag="ACHJ12_002419"
     CDS             15455..15643
                     /gene="dicB"
                     /locus_tag="ACHJ12_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4990190.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            15640..15828
                     /locus_tag="ACHJ12_002420"
     CDS             15640..15828
                     /locus_tag="ACHJ12_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4990191.1"
                     /translation="MTSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            15924..18395
                     /locus_tag="ACHJ12_002421"
     CDS             15924..18395
                     /locus_tag="ACHJ12_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4990192.1"
                     /translation="MNKIFICAAIPDEQAIEEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIACEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NITFDNLAPEVQNAVMVKFDACENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPAAKWPEIQAEMRIWKKRRESERKETGKYISVVDIARS
                     RVNQQNTENAAEKTGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALAYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDFE
                     NQNQPESEQNDQKAEQNSPNALQNEPETKQPEPEEQQEPEKVCTACGQIGGGNCPDCG
                     AVMGDATYQETFDEKNRAEVQEDDPEEMEGAEHPNNENAGNDQHHTSDSETGEAADPL
                     IAVNGHHVITSTSRVWIHLSVDLETMGTNPDAPINSIGGTFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGCFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            18460..18708
                     /locus_tag="ACHJ12_002422"
     CDS             18460..18708
                     /locus_tag="ACHJ12_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4990193.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            18686..19825
                     /locus_tag="ACHJ12_002423"
     CDS             18686..19825
                     /locus_tag="ACHJ12_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4990194.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERY
                     ENGEIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRK
                     VLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQR
                     GMLLALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVV
                     SSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFH
                     EQRSLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(19871..20524)
                     /gene="ompW"
                     /locus_tag="ACHJ12_002424"
     CDS             complement(19871..20524)
                     /gene="ompW"
                     /locus_tag="ACHJ12_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4990195.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            20764..20853
                     /locus_tag="ACHJ12_002425"
                     /pseudo
     CDS             20764..20853
                     /locus_tag="ACHJ12_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            20881..21624
                     /gene="yciC"
                     /locus_tag="ACHJ12_002426"
     CDS             20881..21624
                     /gene="yciC"
                     /locus_tag="ACHJ12_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4990196.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            21654..22193
                     /gene="yciB"
                     /locus_tag="ACHJ12_002427"
     CDS             21654..22193
                     /gene="yciB"
                     /locus_tag="ACHJ12_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4990197.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            22298..22696
                     /gene="yciA"
                     /locus_tag="ACHJ12_002428"
     CDS             22298..22696
                     /gene="yciA"
                     /locus_tag="ACHJ12_002428"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4990198.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYACCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(22736..23455)
                     /gene="tonB"
                     /locus_tag="ACHJ12_002429"
     CDS             complement(22736..23455)
                     /gene="tonB"
                     /locus_tag="ACHJ12_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4990199.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            23679..23975
                     /gene="yciI"
                     /locus_tag="ACHJ12_002430"
     CDS             23679..23975
                     /gene="yciI"
                     /locus_tag="ACHJ12_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4990200.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            24274..25527
                     /gene="kch"
                     /locus_tag="ACHJ12_002431"
     CDS             24274..25527
                     /gene="kch"
                     /locus_tag="ACHJ12_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4990201.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(25582..25755)
                     /gene="yciY"
                     /locus_tag="ACHJ12_002432"
     CDS             complement(25582..25755)
                     /gene="yciY"
                     /locus_tag="ACHJ12_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4990202.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            25898..27358
                     /gene="cls"
                     /locus_tag="ACHJ12_002433"
     CDS             25898..27358
                     /gene="cls"
                     /locus_tag="ACHJ12_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4990203.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            27393..27722
                     /gene="yciU"
                     /locus_tag="ACHJ12_002434"
     CDS             27393..27722
                     /gene="yciU"
                     /locus_tag="ACHJ12_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4990204.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(27775..28779)
                     /gene="oppF"
                     /locus_tag="ACHJ12_002435"
     CDS             complement(27775..28779)
                     /gene="oppF"
                     /locus_tag="ACHJ12_002435"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4990205.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(28776..29789)
                     /gene="oppD"
                     /locus_tag="ACHJ12_002436"
     CDS             complement(28776..29789)
                     /gene="oppD"
                     /locus_tag="ACHJ12_002436"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4990206.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(29801..30709)
                     /gene="oppC"
                     /locus_tag="ACHJ12_002437"
     CDS             complement(29801..30709)
                     /gene="oppC"
                     /locus_tag="ACHJ12_002437"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4990207.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(30724..31644)
                     /gene="oppB"
                     /locus_tag="ACHJ12_002438"
     CDS             complement(30724..31644)
                     /gene="oppB"
                     /locus_tag="ACHJ12_002438"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4990208.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(31730..33361)
                     /gene="oppA"
                     /locus_tag="ACHJ12_002439"
     CDS             complement(31730..33361)
                     /gene="oppA"
                     /locus_tag="ACHJ12_002439"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4990209.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         8698 a         8672 c         7780 g         8640 t
ORIGIN      
        1 aatacccgca ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatgtc
      121 ctgcacatca tgcagacaat agtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 acccggtgtc actgtactgc ggcgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc agcggaaggc cttcaccata tgccatgctg tttgactgcc ccgccagaac
      301 cacaacaaag taatactccg ggtctctggt ggcgctgatt actgtgcctt ctccagccga
      361 cggcttcact aacacaggtg tggtgacatc accttccgcc gcaatggcct gcatcagggt
      421 ataaggcgtg atggcgacag gactgccaaa tggctgccac ccctccttca gtttttgagt
      481 cagtcgctcc gcaaggtctg acggcgacgc cgccctgacc acgtcatagt gtttaaatgc
      541 catgaatcct cccggtcggg ataatgttgt gagtcagatg aggggcgggc tgaagtccgg
      601 aagttacagg acaatggcag gaggaagact acagcccgca atacgaaaaa ggccgcgcag
      661 ttgcgcagag tgattactgt cgggtattat tctccagctg aaaaattgat tacttcacgt
      721 tttgttgttt attccttgct gcccgcatct caccatgcgg gcttttttta tccataagaa
      781 agcccctccg gagaggggct gaagagtagc gctatgtacc gttcattaca tgatgccggg
      841 tgcctcccgg tgagttcagc ccggtgccac cgaacccgcg tcattctcgc taacgatcat
      901 cagagaccat tccgtacacc agccgcccct ccgcacaggg ggattcatca tgcagaaatt
      961 ttttaacaaa ttctcagtcg ggaagacaac cttcaactga ctgaattgtg agcaacatag
     1021 catttaacgg ggaacctgtt ttctgcagta aaaaggccca ccggagcgga cgggcctgga
     1081 aggatagcgg tcatgtgatg ccggtttccc ggtaactcag caccggtatc tgagtcaacg
     1141 ttttctctac tgggtcattt ccgatacgcc ctgcctgctg acaggctttc atcacatctg
     1201 aaaatatagc accctgactg atactgtagt acccaaggtt ccagaaactg tgatgtatcc
     1261 ggcacagaaa agcccctccg gagaggggct gacgctgcgt atctgtatca tcatgcgcat
     1321 ggtgccgggt gcctcccggt gagttcagta tcagcacctg aacccgcaca gaaaggataa
     1381 gggatgtaac caaacacctg tcgctgatat gcccctccgc tcagggggat tcaccatgcc
     1441 agtttctttt aacaaactcc ccgcaaatca gacaactgtc aaccgtctga attgtgagac
     1501 atttaaaatt ttcggggcaa caggaagaca atcatcacga caccggcaac cagcgaccgg
     1561 caccagattt aacgtatgtg cacacaacct gaaccggcat caaaaaacaa accgtgcact
     1621 gacagaaaac gggaaacctc cgtccgctga tcatcactca ttaaggacca cacaaaggcg
     1681 ttatgctcat cttcaagttt ccgtcttctt gtttgcctta caagcaactc agccgtcacc
     1741 tgtacaatgt caatgacctc cttctcttca gcaatgtacg gcctgttcca gttgtctgtg
     1801 ttaatcttct ccgtcaccgg cgctgacttt ttgcaagttt ccccattaaa cccgacgcca
     1861 taaacaacaa catcatcgct ttgcttttcg ttatcaataa tgacgaataa tacagaaacc
     1921 gatgtatcag cgaacccgcc tgaaatctga accaactcaa caacgcggtt gccaaaatac
     1981 tgacgcattg ccagctcacc acgcctgtaa ccaaccccgg gaaagcacaa aaaaaatgaa
     2041 aatcttgcct tcatgctcgc cagaacataa aacgcgtcat ccagaacacc ggaggtcttt
     2101 ttataaggca tcatcgctga aatcttctcc ctgatatccg cgctctgtgc tttgtactgt
     2161 agcgaaaaag gcggattcat gatgacacaa tcgaacgtgt ccgtgtcact gacatcatcg
     2221 atggcgttga agaaattttc attgaccgtc ttcactgtgg catccgggaa attatccggg
     2281 atatgcaaca acggagaggc ctgtatatcc acagcataca gacttcccac cgtgagaaac
     2341 tgctccagtt gcccggagcc acacgcacca tcaaaaatac tcaggggcag atcaccacaa
     2401 tattcccgca ctttttcagc gacaaaccga cgcaaaggaa cggacgtgat gtactctgcg
     2461 tattttctgg cgacatcgcg attattggac tcctgaaaaa ccatttgtga aaggctctgt
     2521 ggttgatatg cgccgggcgt ggcgcggagg caaaaaaggc ccacaaaagc gagccaggaa
     2581 aaataagtgt ggtgcgttgt actggattcg aaccagtgac cgattgctta gaaggcaatt
     2641 gctctgtccg gctgagctaa caacgcagtg tacagataat ggaccgccat cgaggactcg
     2701 aaccccgcgc aaccagcttc gaaggctggc gctctatccc gatgagctaa tggcggtatg
     2761 tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     2821 accactgagc taaagggccg ggcgcaggat aataacggta cgtaactaat cctgcaatat
     2881 catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa acgttcagtc
     2941 tccagctcaa cgccagttgc acgacgcccc agcgccatcg ctgctttgac tgtcgaaccc
     3001 gaccccatga aaaaatctgc aaccaggtca cccggacgac tgctcgcgct gattatctgc
     3061 tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg caccggttta
     3121 tgcgtccaca catcggtgta cggcacctgc gccgtcacac cgaaataccg ccgcagatgc
     3181 ttatattcac tctgcagttc cgtatagtgt cggttcagtt cactgtatgt gctgaccagt
     3241 tggtgatgtg gcttttccag ttctccgcgc tgatgcttct cttctgccac ccgggcaaac
     3301 agcgcctgta atttcagata atcgctttcg tccggtagct gccactgact ggcactgaac
     3361 cagtgcgaca ccatgttttt ctttcctgtg gcatcagcaa tctgttttgc cgttatcccc
     3421 agggcagcac gtgcatcacg aaagtaagca atcagcgggg ccatcacatg ctgtttcagt
     3481 gccctgccct tcgcctcata cccggcatct ttcggacgat acggcccctg ataatgttcc
     3541 gcgaacagaa tgcgctctgt ggcggggaaa tacgccctca ggctttcctt gttgcacccg
     3601 ttccagcgtc cggacggctt cgcccagata atatggttca gcacactgaa gcgttcacgc
     3661 atcatgattt caatgtcaga tgccaggcga tggccacaga acaggtaaag acttccggca
     3721 ggtttcagca cccgccagaa ctgcgccaga cactggtcca gccacttcag gtaatcatcg
     3781 tcgcccttcc actggttatc ccagccctca ggcttcactt taaagtacgg cgggtccgtg
     3841 actatcaggt caacagaatt ttcgggtaac gaccggataa attccaggca gtcggcgttg
     3901 attaactcac aactggatat ttttacagta ttaagcatgg atcattaagc ctgtctctga
     3961 taggctcatt ctgcttttgc gcaaagcagt gggcctgagg tttgcttgtg atccggacgc
     4021 atgagcagat ggctggtgag tgcccctaac acccaccagc cgcccattta ccacaaataa
     4081 aaaagccttc aggactgaag gcgtctgtaa caaccgaact gatagtctgc cagacccgcc
     4141 ataacaagct gggtcagtat taactggcag cgttcacgtg aaagataagt attctgcgca
     4201 atctccccga ctgtcgccgg ttcggtggca cttaattcat taaacaccac tctggcggtt
     4261 tctgtcatat cctgctgttt cagcatgtct ttttcctttt tccggttaac gtgacacacc
     4321 aataactctt gtcaaaaaag ccagcaagct gaaaagacca gtattcgcaa ccaccagcgc
     4381 gtttaacgcc ctgtgtcgtt tttcgggcat aaaaaaaccc gccagtggcg gggatctttt
     4441 gataaaaacg acaaaggcac cattaaggtg ccttttacac aggggcagca ggcttgtcat
     4501 caccttattg ctactcctgg cttccagttc ggaagtctca caagtcccat cagggtacag
     4561 cgacagtatc agtcaacaca ctatgtgtgt caatataaat tcatatcgaa ttcgataatc
     4621 gttttcgatt atcgatacag ataagccatc cctatcccgt gcaataccgc ccgtttaata
     4681 ttctcaaact catcttccgc aatcatagga acaatataat ttcgcccctc ccaggttcta
     4741 gtttttatgc gatccagtcg gctcaatgaa accgtcatca acatgtcaca ttttacccaa
     4801 caagtaatat gctcattacc agggatgggg ttttctgata actcatgatg gcaatctctg
     4861 cgaggaatcg gttcagtagt gcttattggt accactgtta ccaattgatt gttgtgtctg
     4921 tttctggaca cgactaccac tggcctgacc ttgactattt ccggaacaac cattccccga
     4981 aaatcacaca ttaaaacaga acgaacagac ggctgatact ttaacaccat aaatctcttt
     5041 gcctgataat gacgacttgt gaactccact acaaacaaaa tgattctgct gaacaggcag
     5101 tttcgcactg tacattataa cctgttaagc caacaaaaaa acccgctcgg cggcggggtt
     5161 tgcttacttt gccatcgcgt acaaaatcgg caaaatatca gatttacatg aaatatatgc
     5221 tttttaatcc agttttgcaa tattttgctg tgaaaatgtg gtcttttgtt ttgaacgtgt
     5281 tttcgttaaa agcaataaag cttggctatc aagctgtaga aaaatgtgct tcattgcaac
     5341 ccagcgttca gtaaatgtct cagaccagtt ttttgatgtc actcccacca gtgatgccag
     5401 atcccggtat tcataacctt ccccaccaaa aagctcagct tttaccgctt gcgccgccag
     5461 ccagatcaac ttcttcagac gttccagcgt tttcccagct atttttctgg taccggattg
     5521 agtattaaat tcattccacg cccactgcgt tatcgcaatc tgatactccc agcaaatgtt
     5581 tccgccgtaa caccacaaca accacgcctt ctgatgcgct tccagttcca gaactgcacg
     5641 tcgccatgat gatgtcgaaa actcaaccgg actgaccaga gcaattgacg tccctttcgc
     5701 cagtgattgt ttacccggga tcggtgggtt atcccgcgtg atcatttttc cggttacctc
     5761 atcgcggtaa cgaatttttt tgcgtctgta acgccctgta tcgaacagtg cattttcctg
     5821 ccaggcttcc agttggcctt ttatcgacac actgagatcc gcagtggcaa tcatgagttg
     5881 ctcacgaaca aactgtaaat actggttatt catgcacacc cacctctgta attcttatct
     5941 ccagccgtcc accagatact ggctggccac gtacaatatt gatttcatca aactgctcat
     6001 cgtccattaa caaccctgca tgcgtcagcg catccagcgg cgctttcagg atattatcca
     6061 ggtcacggcg gcgcttatcc ggtggctctg caatcacctt tatcgccagc cttccggaca
     6121 ggcttaattt cagccgctgc tggcgaacaa taagcgccac tgcccggcga tagcgctttc
     6181 cctcctcaga tataaaatat gtgctgccac gacgtcgcca gtaagtattc accgtcggcg
     6241 gataaggcaa aacaaactct atacgcatca gtaacctctt ttacccaagc acgccggttg
     6301 caaaggcgtg atcaagaaaa cgaaaaatta actcaacctg agagccgtac tttttctcaa
     6361 actccagcgg gtctgcatga agttcgttgt gatgttcccg gcacaacggt agcgtgaaaa
     6421 tatcgtgggc ctttgtcccc attcccccct gaccatgacc aatcaggtga tgcggatcgt
     6481 cagcaggctt accacaacac gcacacggct gtgtctttac ccagcgcgtg tatttctcat
     6541 taacccaacg gcgacgttta ggccgcttca tgaacgattc aggagactcc ggatcaacag
     6601 cgatactgac aaccgttttt ttctgtggtg gattttgttg ctggtggacg tgaagtggca
     6661 gcgcaatatt ttttgtgcgc tgcttcagta tgctgatggc tgtctgttct cccggtacga
     6721 tgtcactctc acggtatacg gagcggattt tttccgctgg taatcccagc gaacgacgcg
     6781 ctactgcctc aggtaatgca tccaccacct gattgcaggc cgcccaccag gataattcgg
     6841 ccagcgataa ctccctctcc tgcgtaccgc ttattgcatg acggatgacg tcaatcatcc
     6901 aggcaaccag attctgctga gcaagttgat cgagtgattc tgatgtctgg tcgcgcagct
     6961 ggttgtcaca gtgccagcac aacaccatcg cgccggtacc ataacggtga atgacggttt
     7021 cgctgtgatg ataatcgcca tgtggccact ggcaggattt cacgtgacgt aataaccagt
     7081 cagacagcgc accagcaccg ccagcggcac gaatcacccg ttcattgctg aaaaatggca
     7141 gtaatgattt atcctcagcc agcggctggc aaacgacagg aacaaccccg gacggcaaac
     7201 tccgcatgct tttgggttcc ggctccacca gcaccctgcc gcgatgaaaa accggcagta
     7261 attcgcgacc tggcttaatg accaccaggc caagttccgg gaccagaaca ggtcgaagta
     7321 atacccgcac attatctcca gacacgctga cggtagcagg catttgttcg tggcagatgt
     7381 gcgcgaacag gaagatatac agaaacggtc caggtcagac gatcaacgtt cagactccgc
     7441 tccacacgga caccgcgacg cagatacgcc tcttgaagca tatctgcctc atcggtcgta
     7501 cagaacagat agtgaaacca gccatactga ggcgtacgaa aacgccaccc ccgcttaatt
     7561 tccgggtcgg cttcagaatt gtgggatttt atgtgttgtg tcatcggatt ctccggtgac
     7621 agcaggtgtc agttgttcag gctgacggcg cgaattgtaa gtcaatacgc ctggaatgta
     7681 caaacagaaa acccgtcagt aagacgggct tagcaagccg ggacggttac tttaataatt
     7741 tcagtgcctt tacatcaact tcaacactgc tcaggtcttt atcaatttca ccctcaattc
     7801 ttactttgtc tttcggagaa acattctgcc cggcccatac gctgtcatca atatccgtga
     7861 caattgtccc gctattgtca cgaaactcat aacgttcatc acccactttt ttaatgatgc
     7921 tcccttcaag gataacccat gcatcatcct tcagttcttt tgcctgcgct actgttgaac
     7981 gctctgcttc aggcccctgg aaaccaccct gctgtgcaaa aacgccaaaa gacacaccgg
     8041 aaataagtgc tgcaatcaat acctttttca ttcatagtcc tctttcagaa atgaacattc
     8101 aaacagcatt ttcagtatgg taaagcgcgg gtgcgttgag gatgcctgac acatcagagg
     8161 tggcgaggga tttctccctc gcctggtcac tcttacttct cagattcgta gtctacgaag
     8221 acagcaacct ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt cctcgttacc
     8281 agtgccgtca caacaacggt aatacagatg acgatcaggg cgattaacat cgccttttgc
     8341 tgcttcatag cctgcttctc cttgcctttc ggcacgtaag aggctaacct acgtttgtga
     8401 agcatagatt gggcctcaga ttaatgttaa gcgtcctgca agacgcgtaa tgttaactgg
     8461 ggcttttctc tgtctgcctt acggcggcat gcccgaggca gacagcctca agcacccgca
     8521 gcaattctac ttaactcttc tttccccgca aaccgttttt atccccagcg gcaaatcgaa
     8581 tacaccacca gcgccaccgc cattgcaata ccaacatttg agaaggcctc aggccagctc
     8641 attggcgcac ctccttcggc ggttctggta gcggcatcca gtgtgtggct tcacacacga
     8701 taggcgcacc gaaacactgc gcatactgga agtcacccgt attatccagg ttaaaacctg
     8761 taaactgttc ccccgtgctg gacacaaaca gttgtacatc aacaccaact ggcggcattc
     8821 gctcactaca gcttatccaa ccatccggag ttaccggaga attgccagcc ttgcacatgg
     8881 caatctccat gatttcaacc atatctcctg gtggaatttt acaatgctga ccaatatgcc
     8941 tctgctgcct ggcatattcg aggatgtgct ccagtttggt tcgatcaatc atgatttatc
     9001 tccattaagc attgcagcac ggcaatcgtt ccatccttca gcataatcac tatgcgcaag
     9061 aggccaacct cttatgtatt cacgcggcga cttatcaggc actaccagcg ctggcgggac
     9121 ggcaaataga tatccgccaa agtcaggaag ctctccaatg gcctgtacga acttttgttt
     9181 gcctacgtca actcctaatg ggtaatgagc tataatcttt gccactggct ctgcttccag
     9241 cgatgccagt gcgataagcg ccagctcacg aatttcaccg ccgtctatat cgtcaatgtc
     9301 atcgcggcca gaaatgttag ccagccattg cagtcgctct ttggtaatag tggtcatctc
     9361 actctccttc tatgcgaata ccagcagcag tcagtgcggc attgacctca tcagcgtagt
     9421 agtacgttaa tccacttgat gatttagcca gcttgaatgg ttctggtaac ttcactatgc
     9481 gggactcctg ctcgtcgata cgctgttttg cttcctccag ttcatccagc aaatcagcga
     9541 taatatccac ttcacgatga cggatttcac acttaaacgc agccaaagct gcatcacaat
     9601 cctgctcagc gtttggactg tctggtttct cctgatacca cgccagcatc gactgatagt
     9661 tttgtgccgc ctcacgcagc gcctgataat taatcccact cactggttgc ctcctggaaa
     9721 ataactgcat gccccagttt ctccgccagt gccagttctg ccttagcgcc tgccgaccgc
     9781 tgccagcctt tcagcatata aaccgcatcc acacaacgaa tcattgccat gcaaatatcc
     9841 atgtaatgtg gctgagtcag cccgtccgga agtactgacg ggtttaaaac ggtatgccct
     9901 tcccgtttca gtgcttcttc cgccttgtga aatgcctcgc ggttgaaatt ttcatatccc
     9961 gtcattggac cggcgatata aattctcacc ctcacgcctg aaccctcctg tcgaaataaa
    10021 cgtagttatt cactatgcgc agcggcatgc ctaattttct ggcaatttcc cttctttgca
    10081 tgcctctctg atgcagttgc cgcgcctgct caatatcacg ctgagaatat tttgctgact
    10141 ggtgaaaatc accacgtaac atcatgctga tgcccagctc ccgtgccttc gttctcactg
    10201 ccgcttcagt tcgtccgata agtgcgccaa tgctttttac ctttattgtt cccgcgcact
    10261 gccggagtat cagaatttca gcccggcacc acgtcttcca cccactcacc gctgctgttc
    10321 tctggtggcg gtaatatccc ggagaatatc ccggcacttg ttcagctccc acagcgcggc
    10381 gcagacacgc tcccacttct gaacctgacc ttttgcccgg cgcagctcgc ggttggccac
    10441 atgcagcgat ggtaaaatca aaccatcctg atgctttctt gtgaacgacg gctgtgactg
    10501 cactgtgacc gccacacttt cagttttaat ttcttcctgt gtcgtcgttt ccttgactgg
    10561 taacgcaaca cctgctggct gaggaaaggc tttaccatcc gtttccgcta cggatgcagc
    10621 ttccggctct gccggtaaat cagcgcccgg tatgcagtaa cgaaatttac cgccctgatt
    10681 cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg cccggcggga
    10741 ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt gttcaatcgc
    10801 ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac tttcaccagc
    10861 ctgaatcagc caccacatcg accccttgtt atccgcttca ccacggcgct tcagtttcca
    10921 cagttcgttg accgcatctt cacggctgat tccaaggcgg gccgccacta cctgtgaaga
    10981 ggcttttttc agtgctttca gtgcgtcaaa tactgtttcc attaaaattt cctccgacaa
    11041 aatcgtttct cagattcaaa taaaaccagc tgccttccgg cgttcgtatt cctgtttcaa
    11101 ccattcaatt ggcgttggcc ctttcgggtg tttcgcccct tccagttgtc gtcgcactgg
    11161 cggaacactc atcccgttac caacatgctt tgtccatttc gtcagttgcc gttccgcaag
    11221 tcgttttaac tcaccctgcg tcatctggcg ctcaatccct ctggtacgca tttcgaggca
    11281 gatgtggtac agcacaggct gtggccacgg gtatttatca ctcccgtcgt atcgccagga
    11341 ttcattgcgc cagcgccggt actcttccat cacggcatcc accgtaagac caaatggatt
    11401 tgccccactc tccgaaatca gcgcaacaaa ctcagccagg tccgggggcc acgtttcacc
    11461 cgcccggcag cggtccatgc actgacggca gacctgccgg atttgttgtt cagtcatcgc
    11521 accaatctgg gcaatccaga gcttcgaagg tgcggccccg ttcttctgag tccagcggtt
    11581 cgaatacacc tcccccataa gctcccacag cttccaggcc gtttccgttg ctgataaatc
    11641 cgttgtcacg ttcccactgt tcgcgtgctg cccggatttc ctgaactgcc cgtgatgccg
    11701 tgccacctga tgctgcatgg cttaccccct tgctgactgg ttttacctgt gccctgacgt
    11761 gctgcacgtg acgggcaaat ttctgctccc actgaacctg cgtgaaaacc ttcccctccg
    11821 ccatccagta atcccggaat gcggcaagct cagcaggtgt aaattccggc tcaggcagag
    11881 ccatacccca cactgctgcc cgttgtcgaa aatccggcga cggctgccag acagtagtca
    11941 tcgaaaattt cccgatgggt tcgctcaggc cgtccaggta ttcaggttcg gctgtctgca
    12001 acggcgcacc atgcgactca ctggtcggaa tactctcgcg ttcatgcacg ttatgtgtgg
    12061 ggtttaattc ttttagatct gtatctttat tagttgcttt tgtgtttgcg tcatgttcaa
    12121 acacaacacc aacatttgtt tgaacgcctg ttaaatctct ctcttgtttt gtttgaacat
    12181 atgcttcctt tctgcttctt ctggcctgaa cagatgcttt tccggcggct gattttttgg
    12241 ttaatttttc cctgactgat gccagatctt cctcaatccg aagatgcacc cattcatcgc
    12301 cgttatcgca aaaaaactcc cgcaaggatg gttccacatc agcccatcgc tcgttagtca
    12361 gacgggaaat ttttgccagc ctgtttttag gtattggttt ccctgtttgc cagtaattga
    12421 acatcaacaa caaatacgcg ccgtgctcct ctgcggacaa atgcatggtg tcagccaggt
    12481 aatcagctat gtacagttgc atgtatggta atgcggccat aattgcctca tcttgtgacg
    12541 aaccatcctc tggtgatatt ctgtgattcc ccaatcaaca gaatcagcag gggtctggca
    12601 taaatatcaa tgcaccacaa cagactcgcc ggatgacccg ctgtcgctga aatacgcttt
    12661 ccggtaaacg gcctggactg catcatcatg tgcatcaatt gccgtgctca acgcttcctg
    12721 cgccgccagt aatgcacggc gctcaacagt gtcaaaaata ctgaggtgat aacgcagttc
    12781 acgcggaaga acagtcagga tagccggaat tagtgcctga attttttcaa cagcatcagg
    12841 cgtatccttt tcaagccagc gaaaaatatt ttgtatgttc aatccaatac cctctgtcgt
    12901 agaggtgtca tgcattggtg gataagtcat ttcaagctca aaatatgctg tcgctatatc
    12961 atcagcgatt tttttgcgcc ctaccctcgg ataaagcagc cacgcattca ttgccatgcg
    13021 gatatgttca tgcttgattt tcatgaatca agctcctgga aagtggttgt gttaacgttt
    13081 gggtatcttc cagctcaggc caaatgttca tccaatcaaa aggccgtagt tgctgacgtg
    13141 taacttcacc attactggct cgctcaataa ggacacataa cgatgcccct aacacttgac
    13201 ctttactcaa tgcttttctt agataaccga tgctagtacc acactcacat gcaaacatac
    13261 gctgttcatc tgacgaaaga gaattgagaa atattcttaa ttcttccata actactcctt
    13321 agtaaacaca acaaaagaat acccgcaggt aaacaaaagt caatacccct gggttgttta
    13381 ccttgcggta atcgcctcta ttatttacct atggacaaat atgaatttag acgacagcaa
    13441 ctcatcaaaa ttcgtgatga gaaatgcgat ggtaaagcgg ttaacgtggc cagaaaaatc
    13501 ggccgcgagc cttcttatgt atcaagaatg ttgtacccag aggggaaaaa agggaaaaaa
    13561 cggatcgctg atgatatggt ggagattatc gaagaatcct ttgggttacc ccggggatgg
    13621 atggatggta tcgtttcatc atcaacggac acaacctcca attatgaaac aagggttcta
    13681 acaccacgac aacgtatttt tttagatctc ttggacgaac tgccagaaag cgaagcggat
    13741 aacttattaa aaactcttga agagaaaaaa caatattaca atatgatcta cgaagaaatc
    13801 agcaaaaaga aagcacgaaa cgcatcatag cccaccaaac aacctagcac cagtcaagat
    13861 acaccaaaaa acttacccat gggtatttac tttttgaata cccacaggta ttcttccttt
    13921 cattccaacc caccccgtcc cacagaacgc agggcaatac ttagagttac ccggcagtgg
    13981 tcaggggtta agtagccagc ccgaggcgta tgaacatgac ggcgggaaca ctttatataa
    14041 cagcgcagca ggtttttagt tccgcgaccc ggcgttaagg ggaaatgagg tcaacatgga
    14101 tacgctcaat cttggcaaca acgaatctct ggtatgcgga gtattcccca acctagacgg
    14161 cacgtttacc gcgatgacgt ataccagaag caaaacgttt aaaactgaag ctggcgcgcg
    14221 tcgctggtta gccagaaaca ctgactgatg aggttgacga tggaatttaa agatttacca
    14281 gtaccattcc aggaaatggc agcgaatata gttcgttctc aactggcgac tcttgacctg
    14341 agtaccgtag aaaaagaaac catcgataat atatccggta acgtgcgtcg aacctttatc
    14401 gggctgtacg aagaaaagtg gccattcggc ggacaacatt cgcctgaaaa caagaatcaa
    14461 gcgaatgatg agaagctgaa acacgttatc gccttactac tggaagatgc aaaacgtcta
    14521 cagcaactgg aaccaaatgc aggcaccgaa gcccgcatct ggattgccat agaatcactc
    14581 aaatgtgaaa gcgatgatta tttaaaaacc ataattaaaa caactcagct ttctgaagag
    14641 ctaccgaaga aattgccata ataatatgtt tttcttatag aggggttaga aatatgggcc
    14701 agcattatag caggcacata taacagaaag tattttaaat attacgccgt atcttgtgat
    14761 tgttttttaa tatattcata caagcgcaca agttgtttca actcttcaaa gcacatagcg
    14821 gaattttcaa tttttccggc gttgattagt gccagaagag cctaatgtgc gcaagcatat
    14881 ggatcagtta cagggctaat aatatcaact tgcatattat cctccataga ggttccgggt
    14941 taatgatgga gaccaacacg ctgtcacgtg tggtcgtgcg ccggacacgg ataagaatcc
    15001 ggcactgaca gtttgctgaa agaacatatc cctgaaaagt cagggcataa cacgaaagcg
    15061 cacggcgaga atataaggct tgtcgttaaa tttaattcga tcgtgcgctt ccggttgtgg
    15121 caatccgcga aatggcgcgg cggtaagtat ggctggggct tcctccattg ctccagaaaa
    15181 tgcaccgggt tgtcaggttg accatgcgcc tgagtgacaa caccgccaca acaacctctg
    15241 ttatcacttt tctggtgatt cggcggaaat ggatatccgc catttttaaa gtgtattttg
    15301 tgatgcggtg aatgcggcta agcgcacgcg gaacagttaa agctaaaaac agcgttatgg
    15361 gtgattctgt attccggcgt taattgttaa ctggttaacg tcacctggag gcaccaggca
    15421 ccacatcaca aaattcattg ttgaggacgc gataatggaa acgtcactac caaacgttaa
    15481 tacgtctgaa gggtgtttta atattggtat tctgctcagt aaccgggagt ttactgaaga
    15541 cgccatcagg atgagaaaat atgagcctta tcttctcaat gataattcca tactctccag
    15601 aattgccctt cttaaacttg gtattttcgg ggggcagcag tgacttctgc atttgcactg
    15661 atgatgacgg tttttcttat aacaggtgag ccacagaatg tgattaccgg aatttatgcc
    15721 agtaaagaat cctgccatca ggcaagagac gagcaaaaaa tttccggtga atgtctcccg
    15781 ttaaacaaag tatcgctgta cctgaataac gaaacaccgg ctggataacc cgccagccat
    15841 attaacgcca taccaacgga ttaaaaatgc cagcaatggc agggattcgt tcaccctgaa
    15901 atctgtaatg aggttaaaac acaatgaata aaatctttat ttgcgctgct attcctgacg
    15961 aacaggccat agaagaagat agcgctgttg cggtggccac tgccattgaa gccggtgatg
    16021 agcgtcgcgc acgcgcaaaa tttcactggc aatttctgga gcaattcccg gcagctcagg
    16081 actgcgctta taaatttatt gcctgcgagg ataaacccgg cataccccgc cctgccctcg
    16141 attcctggga taccgaatat atgcaggaaa accgctggga tgaggagtct gcgtcctttg
    16201 tcccggtcga acccgaatcc gatccgatga acatcacttt tgacaatctg gcccctgaag
    16261 tacagaacgc cgtcatggtt aagttcgacg catgtgaaaa catcactgtt gatatggtga
    16321 ttagcgctca ggaattgttg caggaagaca tggcaacatt cgacggccat atcgttgaag
    16381 cgttgatgaa gatgccagaa gttaacgcca tgtatccgga gcttaagctg catgccatcg
    16441 ggtgggttaa gcataaatgt aaacctgctg ctaaatggcc cgaaattcag gcagagatgc
    16501 gcatctggaa aaaacgtcgc gaaagtgaac gcaaggaaac cgggaaatac atatctgtcg
    16561 ttgatatcgc ccgttccaga gtaaaccaac agaacactga aaacgctgct gaaaaaaccg
    16621 gggctgtcac tgttgccgtt cgccgcgaat acaaacagac atggaaaaca ctcgacaatg
    16681 aactggcctg cgccctctgg cccggtgatg tggatgcagg aaacattgac ggtaccatcc
    16741 atcgctgggc gacaaatgag gttatcgaca aggatcgcga agactggaag cgtatctcag
    16801 catcaatgcg caaacagccc gaagcacttg cctatgaccg tcagactatt tttggccttg
    16861 ttcgcgaacg tccgatcgat attcacaaag atcccgttgc actgaacaaa tacatcagcg
    16921 aatacctgac gacaaagggc gtgtttgaac atgaagaaac agaccagagc tctgctgatg
    16981 ctatcctgtc gtcagcagca caaactgatc cagtggagac ggcggaatcc aattctcaaa
    17041 aaaatgaaat cctggtggaa gctgaaccat ctgtagagcg tgaaggaccg ttttatttcg
    17101 tctttaccga taaggacggg gaaaaatacg gcagggcaaa caaactttct ggcctggaca
    17161 aggcgctggc tgccggtggt accgaaatct caaaagaaga atattttgcc cgaaaaaatg
    17221 gcacatatac gggcttacag caaaatacag ataccgcaga agattcagaa caaccagagc
    17281 cggtaaaagt taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc
    17341 ctgacaccga taaattgctt gctgcatcac gtggtgaatt tgttgaaggg attagcgacc
    17401 cgaatgatcc gaaatgggtt aaggggattg aaacccgcga ttttgagaac cagaaccagc
    17461 ccgaatcgga acaaaacgac cagaaagcgg aacaaaacag cccaaatgcg ttacaaaacg
    17521 agccagaaac gaaacagcct gaaccagaag agcaacaaga accggaaaaa gtctgcaccg
    17581 cctgcggtca gatcggaggc ggcaactgtc ctgactgtgg cgcggtgatg ggcgacgcaa
    17641 cataccagga aacattcgat gaaaaaaatc gggctgaagt tcaggaagat gatccggagg
    17701 aaatggaagg cgctgaacat ccgaacaatg agaatgctgg taacgatcag catcacacca
    17761 gcgatagtga aactggagag gcggcagatc ccttaattgc ggtgaacggt catcacgtta
    17821 tcacatccac cagcagagtg tggattcacc tgtcggtcga ccttgaaacg atgggaacaa
    17881 atcctgatgc gccaatcaac tctattggcg gtacattttt tgatccggca accggagaga
    17941 tgggacctga attcagtaaa gctatcgatc tggaaacctc cggcggaatc atcgaccgta
    18001 aaacaataaa gtggtgggcg aaacgatcac gcgaagcaca atccgctatt tttaccgatg
    18061 aaatctcgtt agatgttgct ctccggctat tcatagaatt tatcgaaaaa aactccggcg
    18121 ggtgttttgt ccaggtatgg ggaaatggag ccaacttcga caacgtaatt ttacgccgtt
    18181 catatgaacg gcaggggatc ccctgcccgt ggctttacta caacgatcgc gatgtacgca
    18241 caatcgttga actgggtaat gccattggct ttgatgtcag aatggctatt ccattcgaag
    18301 gcgtacccca caatgcgctg gacgatgccc gtcaccaggc aaaacaagtt tcagcaatct
    18361 ggcaaaaact gatcccgagt caggctgatt tttaatgttc aaccccggtc gtcgtccgca
    18421 agctatagtg gcggcggcca tgattagcga acaacgctca tggcaagact tattctgctc
    18481 actgagtggg caaaagagga attcagtgaa ccggtcccta ctccgggcac gttaagtaaa
    18541 tacgctaaag ccggaatgat atttcctctc cccaaaaaag ttggaagaca ctggcgagtg
    18601 gatcctcgag ctcgctttgt cggaatggta aacaagccgg aagtgatcgc cacagatcac
    18661 cctgctttga agaggatact ggaagatggc gcgcccacga aaatataaaa ctgaagttcc
    18721 gggattatct ccgtattttg acaaaagaaa taacaaagtt tactggcgtt acaggcatcc
    18781 cataacagga aaaaatcacg ggctcggcag tattgaccag aaacaggcag aagctattgc
    18841 agcagaagcg aacagccgtc ttgccaggca acaaatggaa caaatggaac aaatgctcac
    18901 tctgcaggag aaaattattc gtgataccgg tggttcatca accgtttctg tttttctgaa
    18961 tagttaccga aaaattcaac aggaaagata tgaaaacgga gagatcaaac tcaacacact
    19021 gaaacagaaa gcatcccctc tcagggtatt tgatgaacgt tttggtacca gacctttaga
    19081 tgccataacc gtaaaagatg tggtatcggt gctggaagat tacaaggcaa aaggacataa
    19141 cagaatggga caaattttca ggaaagtact gattgatgtt ttccgggagg cccagcaaac
    19201 gggcgatgtc ccgcctggct ttaaccctgc agaatcggca aaaaaaccac aggtacggat
    19261 atcacggcaa cgactgacct ttgatgagtg gatgatgatt tataatgcag cggaaaagga
    19321 tggttacttt ttacagcgtg gtatgctgct ggcactgatg acaggccagc gcctttcaga
    19381 tatttgcaaa atgaaatttt cggatatcag ggatggttat cttcatgtcg aacagcaaaa
    19441 aacaggaacc cggattgcca tccctctggc tctgcgttgc gataaattaa atctcaccct
    19501 ggatgatgtg gtgtcatcct gtcgcgattg cgttcttagt ccgtggctat tgcaccacca
    19561 tcatgctaaa gggacagcta agcgcggcgg gatggttaag ccagcaacgt taaccgttgc
    19621 atttaaaaaa gcccgggatt ctgtggatta caactggcgt gctaatggca ccccaccctc
    19681 tttccatgag cagagatctt tatcagaacg attgttcaga gaacagggaa ttgataccaa
    19741 aattttgctg ggccattcga atcaaaaaat gaccgatatt tacaacgacg cacgcggtaa
    19801 ggaatggaaa aaactggtca tttgatgacc agttttgcag aagagttttg cagaggtttt
    19861 gcagagcaaa ttaaaaacgg taccctgcag agaacataaa cacccacgga tcaaggcgta
    19921 cattgtcgct ataactgcct ttagccagtt tacgaccatc agatttatac ttagccgtgg
    19981 tatcgatatc catgtaccac actgacatgt taaccaacca gtcacggtta atcagataat
    20041 caacccccac ctgcccggca gctccccagg aatctttcag actgagatcg gaaagccccg
    20101 cctctttgcc atgatcgtta aatccattat caaagaaggt ggtgtagtta ataccggccc
    20161 caacgtaagg acggaatttg ctgctggcat caccaaaata ccactgcgcc atcagtgttg
    20221 gcggcagatg gtgaacggtt gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga
    20281 acggcgtcgc tgccagtaat tccacaccaa tgttgtcggt cgccatataa gtaaacgtca
    20341 agcccagttg agtgttattg gtcacgctga atccacccag acttcctaac gtaccaccag
    20401 caccttctgt tggacgtacg gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat
    20461 gcgcaaaggc actgccagaa agaagagttg ttactgccaa agccgccact gttaactttt
    20521 tcatatccgc tccgtcgtta tggttataaa aacgtgacga atatacctac attggagtaa
    20581 caaatgatcc tacatagatc acattacata tgataattca ccatttattg atctggatta
    20641 atttagaagt tgcattgaga atcctggtta atgtgattta aatcaatttt taaatatttt
    20701 ctctacgcaa atattaactc gatcgccact tgccgcggtt tgcccacgcc tgaaggccag
    20761 caattacctc cgctttacaa agatatgctt ttccatacaa gacctgatgc tgccacccga
    20821 ggcttaacca gggtacaatt gcccgctaat taacacctgc ataaaactca aggagagtgc
    20881 atgtctatca cggcgcagtc cgtataccgt gacaccggaa atttcttccg taatcaattt
    20941 atgaccattc tgttggtatc gttgctatgt gcgtttatca cagtggtgtt agggcatgtt
    21001 ttctcaccca gtgatgcaca gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt
    21061 agtgggttgt tcgacctggt tcagaatatg tcaccggaac agcaacaaat tttgctgcag
    21121 gcttcagcgg cgtccacttt ttcaggatta atcggtaacg ccattctcgc cggaggcgta
    21181 atattaatta tccagctggt gtctgcgggt cagagagtca gtgcgctgcg agctattggt
    21241 gccagtgcgc cgatattgcc caagttattt attctgattt ttctgactac ccttttagta
    21301 cagattggca tcatgctggt ggtcgttccg ggaattatca tggccatttt actggccctg
    21361 gcaccggtga tgctggtaca ggacaaaatg ggcatttttg cctcgatgcg tagcagtatg
    21421 cggctgactt gggcgaatat gcgtctggtg gcacccgcag tactgagctg gttgctggca
    21481 aaaacactgt tgctgctttt tgcctcttct tttgccgcat taaccccgga aattggtgcc
    21541 gtactggcga acaccttgag caacctgatt tctgccatat tgctcatcta tctgttccgc
    21601 ctgtatatgt tgattcgcca ataaccttaa gcatttgatt acggaatcgt aaaatgaagc
    21661 agtttcttga ttttttaccg ctggttgtct ttttcgcgtt ttacaagatt tatgacatct
    21721 atgcggctac tgcggcgctg atcgtcgcca cggcgattgt gcttatctat agctgggttc
    21781 gctttcgtaa ggttgagaag atggccctga tcacttttgt tctggtggtc gtcttcggtg
    21841 gcttgacgct gttcttccac aatgatgagt ttattaaatg gaaggttaca gtcatttatg
    21901 ctctatttgc gggagccctg ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc
    21961 ggatgctggg taaagaactc acgctgccgc aaccggtatg gtcgaagctg aatctggcct
    22021 gggctgtttt ctttatcctt tgcggtctgg caaacatcta catcgcattc tggctgccgc
    22081 aaaatatttg ggtcaacttt aaagtctttg gcctgaccgc ccttacctta atcttcacat
    22141 tgttaagcgg tatctatatc taccgccaca tgccgcagga agataaatcc taaccagact
    22201 gccagtcctt cggggctggc gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc
    22261 atcgcgcctg tgattttcct tttaagtcgg ttttaccatg tctacaacac ataacgtccc
    22321 tcagggcgat cttgttttac gtactttagc catgcccgcc gataccaatg ccaatggtga
    22381 catctttggt ggttggttaa tgtcacaaat ggatattggc ggcgctattc tggcgaaaga
    22441 aattgcccac ggtcgcgtag tgactgtgcg ggttgaagga atgactttct tacggccggt
    22501 tgcggtcggc gatgtggtgt gctgctatgc atgctgtgtc cagaaaggga cgacatcggt
    22561 cagcattaat attgaagtgt gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta
    22621 taaagcgaca gaagcattat ttaagtatgt cgcggttgat cctgaaggaa aacctcgcgc
    22681 cttacctgtt gagtaatagt caaaagcctc cggtcggagg cttttgactt tctgcttact
    22741 gaatttcggt ggtgccgtta attttaaaca ggatattcac cacaatccca ctgcctggct
    22801 tacccggctc ataacgccat ctgcgcatcg catttttcac ctcacgctca aacatgttcg
    22861 caggcttggc tgagaggatt tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt
    22921 taactttaac ctgcccttca atgcgcaatg cctgtgctcg tgccggatac tgcggctgat
    22981 tacggcttaa tgcgcgtggt cctgaagcca cactggtaac cggcttgctg gttgcagccg
    23041 ttgccgtact tgatgtcagg cgcgcgggtg ccgtattttc aaacggtgat gccggacgcg
    23101 actctacggg tttgatatcg cgttttggct gctcctgtac ctttttcacc ggcttcggtt
    23161 ttggcttagg tttcggcttc ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg
    23221 ggatcggctc aggttccggt tctggctcta ccaccggctc cggtggcggc tgaacggctt
    23281 gtggcggttc gagatcagca ggcgcaacca tcgtgacaga aattggctgc gcaggcgcag
    23341 gtagttcaat aacctgatgt accgaggtat agagcagacc cgccacaaca gcaccatgaa
    23401 tgcagaccga aagtaacgtc ggccagggga agcggcgagg taaatcaagg gtcattgaag
    23461 tcataatcat ttcagtagaa aaaccaggtc tcgattttaa atgcaaatag caatcatatt
    23521 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
    23581 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
    23641 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
    23701 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
    23761 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
    23821 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
    23881 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
    23941 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
    24001 ggtttttgca accgtgttct tgtttggcct ttccttatag ccggtcagtg cattcactac
    24061 cacaagcaga taataacttt atctgtattt ctttatatga tgtgaacata ttcaggaaaa
    24121 tgccctttcc tgacgcagag ttgctcgctc aaaacctttg attactgccg ataaagtcat
    24181 ctactattgt ttaacacatt cacgcgctgc atgtatgttc agaaaatgtg cacgctaaga
    24241 taattaaaca aaattgtggt tacagaggta tcagtgagtc actgggctac attcaaacaa
    24301 acagcaacaa atttatgggt gaccctacgg cacgacattc tcgcgctggc cgtcttttta
    24361 aatggattgc ttatttttaa aacaatctat ggtatgtcgg tcaatttgct tgatattttc
    24421 catatcaaag cattttcaga gttggatctc tccttgctgg caaacgcccc actatttatg
    24481 ctcggcgtct ttcttgtcct gaactccatt ggcttactgt tccgggcaaa gctcgcatgg
    24541 gcaatcagta tcattttgtt gttgatagcg ctaatttaca ccctgcattt ttatccctgg
    24601 ctgaaattta gtattggatt ttgcattttt acgctggtgt ttttgctgat actgcgcaaa
    24661 gacttctccc acagtagcgc cgcagccggg acaatttttg catttattag tttcacgaca
    24721 ttactgtttt actcaaccta cggtgcgctt tatttaagcg aaggttttaa tccgcgaata
    24781 gaaagtttga tgaccgcgtt ctatttttcg atagaaacca tgtcaaccgt cggctatggc
    24841 gatattgtcc ctgtttctga atcagcacga ttgttcacta tttcggtcat tatttccggc
    24901 attaccgttt ttgccacatc tatgacctca atttttggtc cgcttatccg cgggggattc
    24961 aacaaacttg taaaaggaaa caatcataca atgcatcgta aagatcattt tattgtttgc
    25021 ggacattcga ttctcgccat caatacgatc ctgcaactga atcaacgcgg acaaaacgta
    25081 acggttatca gcaacttgcc tgaagatgat atcaagcaac ttgagcaacg cttaggcgat
    25141 aacgctgatg ttatccccgg tgacagtaat gacagttcag tattaaagaa agcgggaatc
    25201 gatcgttgcc gggccattct ggcgctgagt gataacgatg cagataacgc gtttgttgta
    25261 ctctcggcaa aagatatgag cagtgatgtc aaaacagttc tcgccgtcag tgatagcaaa
    25321 aacctgaata agattaagat ggtacatccg gatatcattc tctcaccgca actgtttggc
    25381 agcgaaattc tggcgcgagt tttaaatggt gaagagatta ataatgatat gctcgtttca
    25441 atgttgttga actcaggtca tggtattttc agcgataacg atgaacaaga aacgaaagct
    25501 gacagtaaag aatcggcgca aaaatagagt ttaaagacgg aagagtaaac ttattgggca
    25561 ccgcaatggt gcccagtaca tttagatatt gtagatcgca aacaataacg agttacgctg
    25621 tttgtttaga atgcacttcc tgatgctgtg gatacgcata tttcggcgcg attgcccctc
    25681 aagccaacgc gatttacgtc ggctagcctg acgctgcatg cgccagcgcc ccacttccgt
    25741 tctactccgc ttcatgttaa ctactctatg caataacaga atggtcatta tacccccacc
    25801 cccgacgctg accagtgctt ttcccgcgtt tttatttgcc agatgaatcc atatgcgtaa
    25861 actcataaca atgcgctttc aaaaggattt ctaatctatg acaaccgttt atacgttggt
    25921 gagttggttg gccattctgg gatactggtt gctcattgca ggcgtaactt tacgcattct
    25981 aatgaaacga cgcgcagttc cctccgcgat ggcctggctg ttgattattt acattctgcc
    26041 gttagtcgga attattgcct atcttgccgt tggcgagctc catttaggca aacgccgcgc
    26101 tgagcgcgcc agagcgatgt ggccttccac cgcaaaatgg cttaacgacc ttaaagcctg
    26161 taagcatatc ttcgccgaag aaaatagcag tgtcgctgcg ccattattca agctttgcga
    26221 gcgtcgtcag gggatcgctg gggtcaaagg gaatcagcta caactgatga ccgagtcaga
    26281 tgatgtgatg caggcgttaa tccgcgacat ccagctcgcg cgccataata ttgagatggt
    26341 gttttatatc tggcagcccg gcggcatggc ggaccaggtg gctgaatcat taatggcggc
    26401 tgcacggcgt ggtattcatt gccgattgat gctcgactcc gccgggagtg tggctttttt
    26461 ccgcagcccg tggcccgagc taatgcgtaa tgccggtatt gaagtggtcg aagccttaaa
    26521 ggtcaatctg atgcgtgtgt ttttacgccg tatggacctg cgccaacatc gcaagatgat
    26581 catgatcgat aattacatcg cgtacaccgg cagcatgaat atggtcgatc ctcgctactt
    26641 caaacaagat gcgggcgtag ggcaatggat tgatctgatg gcgcgtatgg aaggccccat
    26701 cgccaccgcg atggggatta tttattcctg cgactgggag attgaaaccg gaaaacgtat
    26761 tctgccgcca ccaccagatg tcaatattat gccgtttgaa caggccagcg gtcacaccat
    26821 tcacacaatt gcttctggcc ccggctttcc ggaagatctc attcaccagg cattattgac
    26881 tgcggcttat tcggcgcgtg aatatttgat catgaccacg ccctactttg tgccaagcga
    26941 tgatttactt catgcgattt gcacggcggc gcagcgcggg gtggatgtca gtattatcct
    27001 tccgcgaaaa aatgactcga tgctggtcgg ctgggccagt cgcgcattct ttacggaact
    27061 gcttgctgct ggggttaaaa tttatcagtt tgaaggcggg ttactgcata ccaagagcgt
    27121 gctggtcgat ggcgaactaa gtctggttgg caccgttaac cttgatatgc ggagtctgtg
    27181 gctgaatttc gagattaccc tggcaatcga cgataaaggt tttggtgcag accttgccgc
    27241 cgttcaggac gattatattt cgcgttcacg tctgctcgat gcccgtttat ggctaaaacg
    27301 tccattatgg caacgtgtcg ccgagcgact gttttacttc ttcagtccgt tgctgtaaaa
    27361 cgtcgccaac agaggttaaa caggtagtga ctatggatat ggatctaaac aatcgcctga
    27421 ctgaagatga aacgcttgag caggcctacg atatttttct cgaactggct gccgacaatc
    27481 tcgatccagc tgatgttctg ctgttcaatc ttcagtttga agagcgcggc ggcgcggagt
    27541 tattcgatcc ggcggaagac tggcaggaac atgttgattt cgatttgaac ccagactttt
    27601 ttgccgaagt ggtgattggt ctggcggaca gtgaagatgg tgaaatcaac gatgttttcg
    27661 cccgcattct gttatgccgc gaaaaagatc acaaactttg ccatatcatc tggcgggaat
    27721 aaataatcgc tttcggcgtg aaaaagggct gacaactgtc agcccttatt gtgtttaaag
    27781 cggatcgact ttcaggcaag aaacggcgtg tctgaaactc ccctccagaa caggacgtgt
    27841 tttggcgcac tccggaccgg caatcgggca acgggtacgg aaaacacaac cggaaggcgg
    27901 gttgatcggc gacggtaatt ccccttccag taactggatg gttttgttct tctccagatc
    27961 cggatcaggt atggggactg ccgacatcaa tgccctggtg taaggatgta gtggattgtg
    28021 gtagacctca tcataggtcc ccagttctac cgcatggccg agatacatca ccaacacacg
    28081 atcggaaatg tgttttacca cggccaggtc atgagcgata aaaattaatg acaatcccat
    28141 ctcacgttgc agctgctgga gcaggttgac cacctgcgcc tgaattgaca cgtccagcgc
    28201 cgaaaccggc tcatcgcaga taatcagctt cggttcaaga ataagagcac gagcaatccc
    28261 gatacgctgg cactgcccac cggagaactc atgcggatag cggttaatca ggttaggcaa
    28321 taacccgact ttcagcatca tcgccttcac gcgctcgcga acttcctggc gtgacatttt
    28381 cggatgataa gtacgcagtg gttcagcgat gatctcgccg atggtcatac gcgggtttag
    28441 cgatgccaac ggatcctgga aaatcatctg aatatcactg cgaacggcac gccattcatc
    28501 gggcttcatg cccagcaact ctttacctaa ccaggcaaca tgaccgtcgg tcgccttgac
    28561 caaaccgatg atggcgcgag caaaggtgga cttaccgcat cccgattccc ctaccacacc
    28621 taatgtttcc ccttcataca ggcgaagagt gacaccatcg acggctttga gcgttttcgg
    28681 cggttgccag aaccactgtt tgccatcttt gatttcaaag tgcactttaa gatcggcgat
    28741 ttcaaggagg acttttcttc cttcagttac agcattcata acagttcctc caccggttta
    28801 aagcaagcac gcagacggcc aggcgtaaac tcttccagcg gcggtgcgct actacaaatt
    28861 tccatcgcat gtggacaacg tggctggaac gggcaacctt tcggtaatcg cagcaagttt
    28921 ggcggattac cagggatggt caacattgtt tcaccttccg catcaagacg cggcaccgcg
    28981 ttgagcaaac cgatagaata aggatgaacg ggttgataaa agacatcgcg cgcattgcca
    29041 tattccatcg tgcgcccggc gtacattacc agcactttgt cgcagatccc cgccaccacg
    29101 ccaagatcgt gggtgatcat aatgatggcg gtattaaatt cccgcttcag ttcatttaat
    29161 agcgtcatga tctgcgcctg tacggtgacg tccagcgcag tagtgggttc atccgcaatc
    29221 agtagcttag gtcgacatag caatgccatc gcaatcatga ctcgctgacg catgccgcca
    29281 gaaaattcgt gcgggtacat tttcatgcgt ttacgcgctt ccggcatttt taccgcatcg
    29341 agcatccgca ccgactcttc aaacgcctca gctttgctca tgttcttatg cagcatcagc
    29401 acttccatta actgctcacc gacgcgcata tagggattca acgaagtcat tgggtcctga
    29461 aaaatcattg agatttgttc agcgcgcagt ttattgagtt catgctctgg caaattgagg
    29521 atttcacgcc cattgaaggt tgccgatccg ccaatacgtc cgttggcagc caacaggccc
    29581 atcaacgcaa atgcagtttg cgatttaccc gaaccagact cacctacaat acccagcgtt
    29641 tctccggcac gtagggaaaa attcaaatca ttgaccgccg tgacgtcgcc gtccggggta
    29701 ctaaaggtga cacgcaaatc tttcacgttc agcagtgcgt cagcctgttg ttgtgcgagc
    29761 ggcacagttg cagtttcaat tacgctcatg gctgcactcc ttaacgatct ttcgggtcga
    29821 gggcatcacg caagccatcg ccgataaagt tgaaacaaaa cagcgtcacc acgaggaatc
    29881 ccgctgggaa caacaataac catggagaga cttccatcga gttcgcgcca tcactcagca
    29941 atgcacccca gctgcttaac ggctcttgcg tacccaaccc caggaagcta aggaaagatt
    30001 caaagaggat catgctgggc accagaagtg atgcgtagac caccaccaca ccgagtacat
    30061 tcggcacaat gtggcgaata acaatgcccg atgtcgatac accgccaact tgtgccgcct
    30121 caataaactc tttgcgcttc agactcaggg tttgcccacg cacaatacga gccatatcca
    30181 gccaggaaac catgcctatc gccacgaaaa tcagcaggat gttttgaccg aaaaaggtca
    30241 ccagcaaaat gacgaagaac atgaatggga aggagttgag gatttccagc agacgcatca
    30301 ttaccgaatc cactttaccg cccagataac cggaaagcga accataaagt gtcccgacga
    30361 ccacagccac cagtgccgca gcaacaccga ccatgagtga gatacgcccg ccaatcgcaa
    30421 cgcgcacaag caggtcgcga ccggatgagt cagtaccaaa gtagtgaccg gactccatat
    30481 ccggggcgct ggacatcatc gcccagtcag tatcgtcata ggcaaactgg gaaagcatcg
    30541 gtgccaggat tacaaataac gcgatcagca ccagcactat cagactggca accgccgcac
    30601 ggttatgcat aaaacgtcga cgtgcgtcct gccacaagct gcgcccttcg acctccagct
    30661 tttcactgaa attttccagc gtctcgctgt ttttcttact taacatcatc gcgagctcca
    30721 gtatcagtaa cggattttcg ggtcgataac cgcatatagc acatcgacaa tggcattaaa
    30781 caaaatggtt aaagcaccaa ccaggatggt caggcttaac actaaggaat agtcacggtt
    30841 caatgcgcca ttaacgaaca gttgcccaat ccccggcaaa ccataaatgg tttcgataac
    30901 catagaaccg gtaataatgc cgacaaatgc ggggcccata taggagagca cgggtaacag
    30961 agcaggtttt aatgcgtggc gtaaaatgat ccgccgcata ggtaaccctt tcgcccgggc
    31021 agtacgaata aaattggagt gtaatacttc aatcatagag ccacgggtaa tacgcgcaat
    31081 actggcgata taggcgagtg acaacgccac catcggcaat atcatgaatt taagcgcccc
    31141 accattccag ccaccgcccg gcagccaatg caaaatgatc gcaaatatca tgactaataa
    31201 tggcgcaacc acaaaactgg ggataacaac cccggtcatt gccagcccca tcacggtata
    31261 gtcccatttg gtgttttgtt ttaatgcggc aataacgcca gcactaacac ccagtattac
    31321 cgcaaggaaa aatgctgcgg ctcccagttt ggcagaaacg ggaaaactgg atgccaccag
    31381 gtcattgacc gaataatctt tatatttaaa cgatggaccg aaatcaccgt gcgccagttg
    31441 tttcaggtag ctgaaatact gtgtcatgat cggatcatta agatgatatt tcgcttcgat
    31501 attggccatc acttcaggcg gtaaagtacg ttcgccggta aaagggcttc ccggcgcgag
    31561 gcgcatcata aagaacgaaa tagtaataag aataaatagc gtcggaatcg cttccagaca
    31621 gcgacgtaga ataaatttta acattgccct accttctggc ctgtgccttc tgtaatgcga
    31681 taaaaatcag acaccgtgga gcaggacact cctgccccac gtattgccat tagtgcttca
    31741 caatgtacat attccgggta taggtattat ccagcggatc tttgccggta tagccaccaa
    31801 cccacggttt caccagacgc gcattcacgt agtaataaac aggaacaatg gccgaatcct
    31861 tatccagctg ttgttctgct ttagtgtaca gagctgtgcg ctgcgcctcg tcagtcactt
    31921 tcagcgtttc cgccataatg ctgtcaaagg ccgggctctt ataatgcgcg gtattcatcg
    31981 agctgttcga aagcatggtg ttcaggaagg aagttggttc gttgtagtca gcacaccagc
    32041 ctgcacgggc cacatcaaaa gtaccctggt gacgggtgtc gaggaacgtt ttccactcct
    32101 ggttaaccag tttgacgttt acaccaatgt ttttcttcca caatgaagag gcagcaatcg
    32161 ccagcttttt atgcagatcg gaggtgttat acaacaggtt gatggtcaac ggtttgtctg
    32221 cggtataacc cgcttcagcc agcagttttt tcgcttcttc gttacgtttt tcctggctcc
    32281 agccaaacca ttccggctga gtcaattttg cgccatcagt atacggtgga gtgtaaccat
    32341 aggcgggcat gtcgccctgc gctttcactt tattaacaat gatatcgcgg tccataccta
    32401 gtttcagtgc ggtacgcaca cgcacatcgt tgaatggcgg tttctggttg ttaatctcgt
    32461 aatagtaagt gcacaggtat ggatcaacgt gaacttcgtc cgggatctct tttttcagct
    32521 tctggaacaa ttcgatcggc atgttgttat aagtcatgtc gatttcacca ctacggtagc
    32581 ggttgacatc ggtaacttca gaagcaatag gcaaataggt tacctggtta ataacggttt
    32641 tcgcgttgtt ccagtaggtc gggctgcgtt caagaacgat tcgttcgttt acgacccaat
    32701 cttttaaggt ataggcaccg ttggtgacga tattaccagg ctgggtccat ttttcgccga
    32761 atttctcgat agcggctttt ggcaccggtg aagttgatgg gtgaacaagt aatttataga
    32821 agtacggaac gggttcactt aaggtgactt ctaatgtgtg atcatcaata gctttcacgc
    32881 cgagatcggt aatcggtttt ttcccttcaa gaatttcatc aataccggcg atatgcccat
    32941 attgcagata actggcatac ggagaagcag tgttcggatc aacagaacgt tgccagctat
    33001 acacaaagtc ttgtgctgtg actggcgtgc catcagacca tttcgcatct ttacgcaaat
    33061 ggaaggtcca gactttcgcg tctttattat cccaggattc agcgacgcca ggtgctggat
    33121 gaccgtcaag atcgctgacc agtaagcctt caaacaggtc tcggctgata ttagactccg
    33181 gaacaccttc aattttgtgc ggatctaatg actgaacttc tgaaccattg ttacgtacca
    33241 gtgtttgttt ttccgccagt gtgacgcctg cgggtacatc agctgccagc gcgacattcc
    33301 ctgccattag cgcagccaga acgccagctg ctactaaact tctcttggtg atgttggtca
    33361 ttgttttttg gactccctca ttataattac tggtattaac cagcatgtgt aatcccggat
    33421 tgggaccctg cacaggctcg gagactttct gctgtcaggt tactttatcg ttacgtatta
    33481 ctatcaccga ctttatttat tctggtgatt cagacgatca ccttatgtcg attcgtactg
    33541 tgtctcccct gtcgagacac attattcttt cgcgacgctc tttaattgtt aattattctc
    33601 attaccaata aagaatcgtc tggcggttgc ccggaaagta ccaaatgcgg ttcttactcg
    33661 ccaatacatt ttgcaaaatt gttaagcaat tctcttttat tgaccgtatc agaatctata
    33721 gcttgcatcc atgccttaac aaacatcttt caattcaatc aaatagagat tggctaagca
    33781 tgtctatctt
//
LOCUS       DAZFFI010000044        32651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFFI010000044 DAZFFI010000000
VERSION     DAZFFI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.044"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..1365
                     /gene="hpaG"
                     /locus_tag="ACHJ12_002440"
     CDS             76..1365
                     /gene="hpaG"
                     /locus_tag="ACHJ12_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO4990210.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1362..2828
                     /gene="hpaE"
                     /locus_tag="ACHJ12_002441"
     CDS             1362..2828
                     /gene="hpaE"
                     /locus_tag="ACHJ12_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4990211.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2830..3681
                     /gene="hpaD"
                     /locus_tag="ACHJ12_002442"
     CDS             2830..3681
                     /gene="hpaD"
                     /locus_tag="ACHJ12_002442"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO4990212.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3691..4071
                     /locus_tag="ACHJ12_002443"
     CDS             3691..4071
                     /locus_tag="ACHJ12_002443"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO4990213.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4182..4985
                     /gene="hpaH"
                     /locus_tag="ACHJ12_002444"
     CDS             4182..4985
                     /gene="hpaH"
                     /locus_tag="ACHJ12_002444"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO4990214.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVVLGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4996..5784
                     /gene="hpaI"
                     /locus_tag="ACHJ12_002445"
     CDS             4996..5784
                     /gene="hpaI"
                     /locus_tag="ACHJ12_002445"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO4990215.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5959..7335
                     /gene="hpaX"
                     /locus_tag="ACHJ12_002446"
     CDS             5959..7335
                     /gene="hpaX"
                     /locus_tag="ACHJ12_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO4990216.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQSSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7345..8235
                     /gene="hpaA"
                     /locus_tag="ACHJ12_002447"
     CDS             7345..8235
                     /gene="hpaA"
                     /locus_tag="ACHJ12_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO4990217.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8486..10048
                     /gene="hpaB"
                     /locus_tag="ACHJ12_002448"
     CDS             8486..10048
                     /gene="hpaB"
                     /locus_tag="ACHJ12_002448"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO4990218.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10066..10578
                     /locus_tag="ACHJ12_002449"
     CDS             10066..10578
                     /locus_tag="ACHJ12_002449"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO4990219.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKMCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10971..13121
                     /gene="btsT"
                     /locus_tag="ACHJ12_002450"
     CDS             10971..13121
                     /gene="btsT"
                     /locus_tag="ACHJ12_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4990220.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13171..13374
                     /gene="yjiX"
                     /locus_tag="ACHJ12_002451"
     CDS             13171..13374
                     /gene="yjiX"
                     /locus_tag="ACHJ12_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4990221.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13385..14341
                     /gene="yjiA"
                     /locus_tag="ACHJ12_002452"
     CDS             13385..14341
                     /gene="yjiA"
                     /locus_tag="ACHJ12_002452"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4990222.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14419..16959)
                     /locus_tag="ACHJ12_002453"
     CDS             complement(14419..16959)
                     /locus_tag="ACHJ12_002453"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO4990223.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIDALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESSYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGATAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPLSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVDTLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17311..17475)
                     /gene="yjiS"
                     /locus_tag="ACHJ12_002454"
     CDS             complement(17311..17475)
                     /gene="yjiS"
                     /locus_tag="ACHJ12_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4990224.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17652..19064
                     /gene="yjiR"
                     /locus_tag="ACHJ12_002455"
     CDS             17652..19064
                     /gene="yjiR"
                     /locus_tag="ACHJ12_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4990225.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYCMKIQVAAGSIFSASGKYRNCLRINCALPL
                     NETYREALKQIGEAVYQAME"
     gene            complement(19307..20251)
                     /locus_tag="ACHJ12_002456"
     CDS             complement(19307..20251)
                     /locus_tag="ACHJ12_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO4990226.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMELIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQQRERIMTIAERIHNDGWLLGREKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSAEQMQALDQPLPESKRDPWIEY"
     gene            20718..21950
                     /gene="mdtM"
                     /locus_tag="ACHJ12_002457"
     CDS             20718..21950
                     /gene="mdtM"
                     /locus_tag="ACHJ12_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4990227.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGATTISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGIIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21991..23271
                     /gene="yjiN"
                     /locus_tag="ACHJ12_002458"
     CDS             21991..23271
                     /gene="yjiN"
                     /locus_tag="ACHJ12_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4990228.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNERHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23387..24538
                     /gene="yjiM"
                     /locus_tag="ACHJ12_002459"
     CDS             23387..24538
                     /gene="yjiM"
                     /locus_tag="ACHJ12_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4990229.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTIEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24548..25315
                     /gene="yjiL"
                     /locus_tag="ACHJ12_002460"
     CDS             24548..25315
                     /gene="yjiL"
                     /locus_tag="ACHJ12_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4990230.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25312..25569
                     /gene="ytiA"
                     /locus_tag="ACHJ12_002461"
     CDS             25312..25569
                     /gene="ytiA"
                     /locus_tag="ACHJ12_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4990231.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25634..26494
                     /gene="yjiK"
                     /locus_tag="ACHJ12_002462"
     CDS             25634..26494
                     /gene="yjiK"
                     /locus_tag="ACHJ12_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4990232.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26562..27740
                     /gene="yjiJ"
                     /locus_tag="ACHJ12_002463"
     CDS             26562..27740
                     /gene="yjiJ"
                     /locus_tag="ACHJ12_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4990233.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27753..28307)
                     /gene="kptA"
                     /locus_tag="ACHJ12_002464"
     CDS             complement(27753..28307)
                     /gene="kptA"
                     /locus_tag="ACHJ12_002464"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO4990234.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28557..29240
                     /gene="yjiH"
                     /locus_tag="ACHJ12_002465"
     CDS             28557..29240
                     /gene="yjiH"
                     /locus_tag="ACHJ12_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4990235.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29237..29698
                     /gene="yjiG"
                     /locus_tag="ACHJ12_002466"
     CDS             29237..29698
                     /gene="yjiG"
                     /locus_tag="ACHJ12_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4990236.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGTLTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29711..30883
                     /gene="iadA"
                     /locus_tag="ACHJ12_002467"
     CDS             29711..30883
                     /gene="iadA"
                     /locus_tag="ACHJ12_002467"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4990237.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30948..31859
                     /gene="hypT"
                     /locus_tag="ACHJ12_002468"
                     /gene_synonym="qseD"
     CDS             30948..31859
                     /gene="hypT"
                     /locus_tag="ACHJ12_002468"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO4990238.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31852..32244)
                     /gene="iraD"
                     /locus_tag="ACHJ12_002469"
     CDS             complement(31852..32244)
                     /gene="iraD"
                     /locus_tag="ACHJ12_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4990239.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPLSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(32241..32324)
                     /gene="idlP"
                     /locus_tag="ACHJ12_002470"
     CDS             complement(32241..32324)
                     /gene="idlP"
                     /locus_tag="ACHJ12_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4990240.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32440..32583)
                     /gene="ytiD"
                     /locus_tag="ACHJ12_002471"
     CDS             complement(32440..32583)
                     /gene="ytiD"
                     /locus_tag="ACHJ12_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO4990241.1"
                     /translation="MDDYAEINNFPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         7519 a         8620 c         8533 g         7979 t
ORIGIN      
        1 ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta aaatgatcga
       61 atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca tcgcagccaa
      121 cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc taaaactgcg
      181 gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat tccctttcca
      241 cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa aacggcgacg
      301 aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc taacgacgtc
      361 agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg tgatggattc
      421 tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat ctataccgag
      481 atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa cgccgcgcag
      541 ttgctgagcg ccctgagcga atttgccacg ctgaatccag gcgacgccat tctgctcggc
      601 acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct cgcagaaggt
      661 ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg caagagcttc
      721 ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta cgccgaccac
      781 gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa agcgccaaat
      841 accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata catgcactat
      901 gaagcggagc tggtggtagt tattggcaaa caggcgcgta acgtcagcga agccgatgcc
      961 atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga ctatctggaa
     1021 aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc gatgctttca
     1081 accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg caccttcgtc
     1141 aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt gcccttcctg
     1201 atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc caccggcaca
     1261 ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg
     1321 ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt
     1381 ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gaccaccaat ccggcaacgg
     1441 gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg
     1501 cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt gcgcgcctga
     1561 tgcgccgtct gggcgatctg atcgaccaga acgtgccaga gatcgccgcg atggaaaccg
     1621 cggacaccgg cctgccgatc catcagacca aaaatgtgtt gatcccacgc gcttcccaca
     1681 actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat ccggttgacg
     1741 acaagatgct caactacacg ctggtgcagc cggtgggagt ttgtgcgctg gtatcgccgt
     1801 ggaacgtacc gtttatgacc gccacatgga aggtcgcgcc gtgtctggcg ctgggcaata
     1861 ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc tgaccgcctg ggtgagctgg
     1921 cgctggaagc cggtattccg gcaggcgtgc tgaacgtggt acagggctac ggcgcaaccg
     1981 caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc ggcggtacgg
     2041 ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg gaactgggcg
     2101 gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgcgcgctg gacgccgccc
     2161 tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc atctttattc
     2221 agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg
     2281 tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tattagccag cagcactggg
     2341 aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg ctggcgggcg
     2401 gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa
     2461 ccgtgctggc agacgttgat aaccgtatgc gagtcgccca ggaagagatt ttcgggccgg
     2521 tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgtctggca aacgacgtgg
     2581 agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta cgcctggcgc
     2641 gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgac ctgcgccagc
     2701 catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag
     2761 tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt ccgaaatggg
     2821 gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc gtcgatgtat
     2881 ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga cgggcataaa
     2941 gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt cgatacccac
     3001 tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg cgtctacacc
     3061 agcaacgagc taccgcattt tatccgcgat atgacctaca actacgaggg caacccggag
     3121 ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa agcgcacaac
     3181 attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat gaatgaagac
     3241 aagcacttca aagtggtttc catttcagct ttctgcactg ttcacgattt tgccgacagc
     3301 cgcaagctgg gcgaggcgat tctgaaagcg atcgaacagt acgacggcac cgtggcggtc
     3361 cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgagcgga agaagggatg
     3421 aacagctaca cccgcgagtt cgaccgccag atggacgagc gcgtggtgaa gctgtggcgc
     3481 gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta ctgctacggc
     3541 gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga taaatacgac
     3601 ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca ggttaacgct
     3661 gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgttga atgcagtgac
     3721 aacatccgcg aagaagccga cctgccgggg ttgttcgcca aagtgaatcc gacgctggca
     3781 gccacgggta tttttccgct ggcgggtatt cgcagccgcg tgcattgggt cgatacctgg
     3841 cagatggccg acgggcagcg tgattatgcc ttcgtgcata tgacgttgaa aatcggcgca
     3901 ggtcgcagcc tggaaagccg tcagcaggcg ggtgaaatgc tgtttgaact gattaaaacg
     3961 cacttcgccg ccctgatgga gagccgcctg ctggcgttgt cgtttgagat tgaagagctg
     4021 catccgacgc tgaattttaa acaaaacaac gtgcacgcat tgtttaagta gcgcgcagat
     4081 tgcccggtgg cgctgcgctt accgggccta caaaacccca aaccgtacac cgtaggccgg
     4141 ataaggcgca gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacataccc
     4201 acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga
     4261 tctcactgga ttacccggag atcaccatcg aagacgctta cgcggtgcag cgcgaatggg
     4321 ttcgcctgaa aatcgccgaa ggtcgcacgc tgaaaggcca caaaatcggc ctgacctcga
     4381 aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggcgcgctg ctggatgaca
     4441 tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag
     4501 tggagctggc tttcgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg
     4561 tctacaacgc cacggactac gtgatcccgg cgctggagct gatcgacgct cgctgccaca
     4621 acatcgatcc ggaaacccag cgcccgcgca aagtgttcga caccatttct gataacgccg
     4681 ccaacgccgg ggtggtcctc ggtggtcgtc ccattaagcc cgatgagttg gatctgcgtt
     4741 ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg
     4801 tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg
     4861 tacaactgga agccgggcaa atcattctcg gcggttcgtt cacccgcccg gttccggcgc
     4921 gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg
     4981 tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgcccgcag
     5041 atcggattat ggctggggct gagtagcagc tacagcgcag agttactggc cggagcagga
     5101 ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc
     5161 cagctacagg cgattgcgcc ctaccccagc cagccggtgg tacgtccgtc gtggaacgat
     5221 ccggtgcaaa tcaaacaact gctggacgtc ggcacacaaa ccttgctggt gccgatggta
     5281 caaaacgctg acgaagcccg tgaagcggta cgcgccaccc gttatccccc cgccggtatt
     5341 cgcggtgtgg gcagtgcgct ggcccgcgcc tcgcgctgga atcgtattcc tgattacctg
     5401 caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag
     5461 aacttaccgc agattctgga cgtggaaggc gtcgacggcg tgtttatcgg cccggcggat
     5521 ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt
     5581 gagcaggcga tcgtgcagat ccgtgaatcg ggcaaagcgc cggggatcct gatcgccaat
     5641 gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac
     5701 accaccctgc tcgcccgcgc cgctgaagcg ctggcagcac ggtttggtgc gcaggccacc
     5761 gccgtgaagc ccggcgtgta ttaatgccgg atgcgctgcg cttatccggc ctacactcgc
     5821 accaaaccgt aggccggata aggcgtttac gccgcttccg gcaaaaagct gtaccaaatc
     5881 gcggaccgga taaggcgtta cgccgcatcc gccataaacc ttgtcgtacc ctacaaaaat
     5941 cccattagag gaagaaaaat gagcgacacc tcacctgcca taccggagag tatcgatccg
     6001 gcgaatcagc ataaagcgct gactgccgga caacaggcgg ttattaagaa gctatttcgc
     6061 cgcctgatcg tctttctgtt cgtgctgttt atcttctcgt tccttgatcg catcaacatc
     6121 ggctttgccg gactcacgat gggacgcgac ctcggtctga gcgccaccat gtttggcctc
     6181 gctaccaccc tgttctatgc cgcttatgtc atcttcggca ttcccagcaa cattatgctg
     6241 agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg tgctctgggg catcgcctct
     6301 actgccacca tgtttgccac tggccccacc agcttgtacg tactgcgtat actggttggc
     6361 attaccgaag ccggctttct gcctggtatt ctgctgtatt taaccttctg gtttccggcc
     6421 tatttccgcg cccgtgccaa cgccttgttt atggtagcaa tgccggtaac gacagcgttg
     6481 ggctcgatcg tttccggcta cattttgtcg ctggatggcg tcatggcatt aaaaggctgg
     6541 cagtggctgt ttttgctgga aggcttcccg tcggtattac tcggcgtcat ggtgtggttc
     6601 tggcttgatg actcaccgga caaagctaag tggctgacga aagaagacaa aaaatgcctg
     6661 caagagatga tggataacga tcgtctgacg ctggttcagc cagagggagc catcagccac
     6721 cacgccatgc aacaacgcag tatgtggcgg gagatcttca ctccggtggt gatgatgtat
     6781 accctggcgt atttctgcct gaccaacaca cttagtgcga tcagcatctg gacaccgcag
     6841 atcctgcaaa gttttaatca gagcagcagt aatatcacca tcggcctgct ggccgccgta
     6901 ccgcagattt gtaccattct cgggatggtc tactggagcc gtcactcaga tcgccgccag
     6961 gaacgaaggc atcacaccgc ccttccttat ttgttcgctg ccgcaggttg gttactggct
     7021 tcggcaactg atcacaacat gatccagatg ctggggatca ttatggcttc gaccggatca
     7081 ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt ccatcagcct gcgggcacga
     7141 gcgatcggta ttgcggtgat caacgccact ggcaacattg gttcagcgtt aagtccgttt
     7201 atgatcggct ggttgaaaga tttgaccggc agctttaaca gtggattgtg gtttgttgcc
     7261 gcgctgctgg tgattggtgc ggggattatc tgggcgattc caatgcagtc ctcccgtccg
     7321 cgagcgaccc cgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat tgatatcagc
     7381 aaagagtacg atgaaagcct gggcacggac gatgtgcatt atcagtcctt cgcccgcatg
     7441 gcggcctttt ttggccgcca tatgctgcca catcgccacg aacagtactt tcagatgcat
     7501 ttcctcaata gcggacagat tgagctacag cttgacgatc atcgctactc ggtggaagcg
     7561 cccctgtttg tcctgacgcc gccgtcagta cctcatgcgt ttattacgga gtctgacgcc
     7621 gacggtcatg tgttgacggt acgggaagat ctgatctggc ccctgctgga agttctttat
     7681 ccgggcactc gggaaacctt cggcctgccg gggatttgcc tgtcactggc agataaaccc
     7741 gacgaattgg cggcgctgga acactattgg caactgatag agcgggaatc ggtggaacaa
     7801 ctgcctggac gggaacacac cctgacgtta ctggcacagg cagtgttcac cctactgctg
     7861 cgtaacgcaa aactcgacga ccatgccgcc agcgggatgc gcggagaatt aaaactgttc
     7921 cagcgtttta atatgctgat tgaaagccat tttcatcagc actggacagt accggattac
     7981 gctaacgaac tgcatatcac cgaatcacga ctcacggaca tctgccgtcg ctttgccaac
     8041 cgtccgccaa aacggttgat tttcgacagg cagctacgag aagccaagcg gctgctgctg
     8101 ttttctgata acgccgtgaa caatattgcc tggcaactcg gttttaaaga tccggcttat
     8161 tttgcccgct tttttaatcg cttagtcggt tgctcgccca gtgcttatcg tgccaaaaaa
     8221 gtacctgtga cgtgaaaatc ccttagcctt aacgggaaac caggcaccac ctgctattcc
     8281 cctttctccg cctggagcaa ggggatttag taacgtcaaa aatcgctaaa agcgacctcg
     8341 atcacaaaac cgtacccgac ctgaaaagta ccagccattg tcccaaaagt ctcttcccgt
     8401 aatcgcccct gcaagcttca attgaataac aaaaacaaca cacaaaatta acaatcgtat
     8461 tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca gtacccaacg
     8521 tccgttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga tctatatcta
     8581 tggcgagcga gtgaaagacg tcaccactca tccggcattt cgtaatgcgg cagcgtctgt
     8641 tgcccagctg tacgacgcac tgcacaaacc ggagatgcag gactctctgt gctggaacac
     8701 cgacaccggc agcggcggct atacccataa attcttccgc gtggcgaaaa gtgccgacga
     8761 cctgcgccag caacgcgacg ccatcgctga gtggtcacgc ctgagctatg gctggatggg
     8821 ccgtacccca gactacaaag ctgctttcgg ttgcgcactg ggcgcgaatc cgggctttta
     8881 cggtcagttc gagcagaacg cccgtaactg gtacacccgt attcaggaaa ctggcctcta
     8941 ctttaaccac gcgattgtta acccaccgat cgatcgtcat ttgccgaccg ataaagtaaa
     9001 agacgtttac atcaagctgg aaaaagagac tgacgccggg attatcgtca gcggtgcgaa
     9061 agtggttgcc accaactcgg cgctgactca ctacaacatg attggcttcg gctcggcaca
     9121 agtgatgggc gaaaacccgg acttcgcact gatgttcgtt gcgccaatgg atgccgatgg
     9181 cgtgaaatta atctcccgcg cctcttatga gatggtcgcg ggtgctaccg gctcgccata
     9241 cgactacccg ctctccagcc gcttcgatga gaacgatgcg attctggtga tggataacgt
     9301 gctgatccca tgggaaaacg tactgatcta ccgcgatttc gatcgctgcc gtcgctggac
     9361 aatggaaggc ggttttgccc gtatgtatcc gctgcaagcc tgtgtgcgcc tggcagtgaa
     9421 actcgacttc attacagcac tgctgaaaaa atcactcgaa tgtaccggca ccctggagtt
     9481 ccgtggtgta caggccgatc tcggtgaagt ggtggcgtgg cgcaacacct tctgggcatt
     9541 gagtgactcg atgtgttctg aagcgacgcc gtgggtcaac ggggcttatt taccggatca
     9601 tgccgcactg caaacctatc gcgtactggc accaatggcc tacgcgaaga tcaaaaacat
     9661 tatcgaacgc aacgttacca gtggcctgat ctatctccct tccagtgccc gtgacctgaa
     9721 caatccgcag atcgaccagt atctggcgaa gtatgtgcgc ggttcgaatg gtatggatca
     9781 cgtccagcgc atcaagatcc tcaaactgat gtgggacgcc attggcagcg agtttggtgg
     9841 tcgccacgaa ctgtatgaaa tcaactactc cggtagccag gatgagattc gcctacagtg
     9901 tctgcgccag gcacaaagct ccggcaatat ggacaagatg atggcgatgg ttgatcgctg
     9961 cctgtcggaa tacgaccaga acggctggac tgtgccgcac ttgcacaaca acgacgatat
    10021 caacatgctg gataagctgc tgaaataacg cagcaggagg ttaagatgca attagatgaa
    10081 cagcgcctgc gctttcgtga cgcgatggcc agcctgtcgg cagcggtaaa tattatcacc
    10141 accgagggcg acgccggaca atgcgggatt acggcaacgg ccgtctgctc ggtcacggat
    10201 acaccaccat cgctgatggt gtgcattaac gccaacagtg cgatgaaccc ggtttttcag
    10261 ggcaacggta agatgtgcgt caacgtcctc aaccatgagc aggaactgat ggcacgccac
    10321 ttcgcgggca tgacaggcat ggcgatggaa gagcgtttta gcctctcatg ctggcaaaaa
    10381 ggtccgctgg cgcagccggt gctgaaaggt tcgctggcca gtcttgaagg tgagatccgc
    10441 gatgtgcagg caattggcac acatctggtg tatctggtgg agattaaaaa catcatcctc
    10501 agtgcagaag gtcacggact tatctacttt aaacgccgtt tccatccggt gatgctggaa
    10561 atggaagctg cgatttaagc tccagcccct tcacgcattc gtcgaagggg catttttatt
    10621 gtgatctcaa tcacatatcc acccgatgaa taattcttgc ccaaccgctc accgccattc
    10681 ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
    10741 actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
    10801 tagaacggct tcggcctatg attaacaaat tactctatga attttggttt gcaggaaggc
    10861 agcaagtgag tgaatccccg ggagcttaca acagtaagtg acaggggtga acgaacgcaa
    10921 ctgccgcacc tgtaagccaa aagacgacga gtaaaatgcc aggttttact atggatacga
    10981 aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg
    11041 cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg
    11101 tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag aaggtgatga
    11161 aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc
    11221 cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc
    11281 tggtcggtcc ggttctcgcc gcacagatgg gctacctgcc tggcacgctg tggctgctgg
    11341 cgggggtagt gctggccggt gcggtacagg actttatggt gctgtttatc tcctctcgcc
    11401 gtaacggcgc atctctcggt gagatgatca aagaagagat ggggccagta ccggggacta
    11461 tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct tgccgtcctg gcgctgattg
    11521 tggttaaagc cctggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga
    11581 ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct
    11641 ctgtcattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg
    11701 atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg
    11761 gctatgcgtt tgtttccgca ttgctgccgg tgtggctgat cctcgcaccg cgcgactatc
    11821 tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga
    11881 acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga
    11941 aaggtgctct gttcccgttc ttgttcatta ccatcgcctg tggtgcggta tctggcttcc
    12001 acgcactgat ctcttccggt acgacgccga aactgctggc taatgaaacc gacgcgcgtt
    12061 tcattggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgctg
    12121 cgtccatcat cgaaccgggt ctttacttcg cgatgaacac cccgcctgct ggccttggca
    12181 tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc
    12241 tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc
    12301 cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag
    12361 gtggtgcgcc aacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg
    12421 ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg
    12481 cgctggatgc gggtactcgt tctggccgct ttatgctgca agacctgctg ggtaacttca
    12541 tcccgttcct gaaaaaaacc gactctctgg ttgccggtat catcggtact gcgggctgtg
    12601 tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga
    12661 gcctgtggcc gctgttcggt atttccaacc agatgctggc agccgtagcg ctggtactgg
    12721 gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg
    12781 ctgtatggct gcttatctgc accacctggg cgctgggcct gaaactgttc agcaccaacc
    12841 cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta
    12901 ctgacctgac ggcgcagcag attgccaaca tgaaccacat cgttgtaaac aactacacca
    12961 acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca
    13021 aaacctggct tgcggtgcgt aacagcgaca aacgtactga caaagaaact ccgtacgttc
    13081 caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta gccccgcttc
    13141 ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa
    13201 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actatgacaa ctacgtcgag
    13261 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    13321 cgccagaacg cacgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    13381 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac
    13441 cctgctgcgc catattctta atgaacaaca cggctacaag attgccgtga ttgaaaacga
    13501 attcggcgaa gtctccgttg atgatcaatt gattggtgat cgcgcaacgc agatcaaaac
    13561 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaggacg cgctactcga
    13621 cctgctggat aatctcgaca agggcaatat tcagttcgat cgtctggtca ttgaatgcac
    13681 cggcatggcc gatcctggcc cgattattca aacctttttc tcccatgaga ttttatgcca
    13741 gcgctacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    13801 gaaccagttc accatcgccc agtcccaggt gggctacgcc gaccgtattc tgctgactaa
    13861 aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgt ctggcacgca tcaacgcccg
    13921 tgcaccggtc tacaccgtca cccacggcga catcgacctg ggtctgctgt tcaacaccaa
    13981 cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccatt ttatcgcgga
    14041 caaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag atatcagcga
    14101 agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc tgcgttacaa
    14161 agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg tccagcgcct
    14221 ctacagcgcc gactgggaca ggccatgggg cgatgaaaaa ccgcatagca cgatggtgtt
    14281 tatcgggatt cagttgccgg aggatgagat tcgggcggcg tttgcgggac tcaaaaagta
    14341 accagccctc caacatcaca atacctgaga ctttatcacc ctcatattcc tccagaatat
    14401 gagggcattc gggcttaatt atctctgaac gtgcaacact tcctgcgact ccttatgcgc
    14461 ctccacatcc tgcttcgtta accagagcga cttacgacca aaattttcgt acattttcag
    14521 ctgatcttca tagtgcttat caactgttcc atcgggagca ataaacccac tctgaccggg
    14581 tgcgaccaca tcccaggcaa gcacaggacg atcgcttgtc gttggtgaga aaacaatcat
    14641 atcgttttct gttccacggt tttgatactc cgcctgatga cgcgtttctt ccgctgcggc
    14701 ctgcggtaca ccaaagaaat tatttgcccg gaacgttaag gccattgcag gtgttttcca
    14761 gttactcaca ttattgccat agcgtttgga aagagtctcc caggtatctt ccagcgcagc
    14821 caacacaacc tcctgctgtg gtttcccagc aaacagatca accgcctgtg ggattggtga
    14881 tttgtctccc tgcaccgcct catacaaaat ttttgctcca acacttatat tcagcgaacc
    14941 agttgggccg tcctgggttg tttcgtagcc actggcgctg taccacttat caaatggcat
    15001 aggtacggca gccactacgg tacgcttcaa catactggtc agccaaacgt tcaggatggc
    15061 agagcctggc tgctgccagg ttttaccatc atcattaagc aaattgatgc catcccaacg
    15121 tgttaatgta tccaccaact gacggcgcgg atcgctctgt gtcaaaccag atgtcgctgc
    15181 ttgcagagta ggtaaaaaaa gcctcaggtt aagatcctga cgactggttt ggcgaataac
    15241 atcccatgcc tgatcagcag ttaagcgtgg cttttgctca agcagtcggt cgatctccgt
    15301 aacgcgatct gccccccccc acaaaaaggc aaacagatcc gaagcgggat aatctttttg
    15361 gggagaattg ttccagttag caatatatcc cgacaggggg ttatacacct tagggttcat
    15421 ttcaaaaggc aatagccctt tccagtccca ttttcccgta ccaggaacgg gtaatcgcgg
    15481 atcatggcct gattgacgat ctggataagc accagtatga acataaccaa tattgccgtt
    15541 tacatcagca tagtaccagt tgatggtcag tgcttgtttc gctgcctgct gtgtccactc
    15601 ctgccaattt ttggccttca tctgatgagt ccaggccagc aaagacgcca cctctttacc
    15661 atcccatgcg cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt
    15721 gccatgcacc gtacgccaga cagtaaaggt ctctgcctga ccatttttca ccgtaatggt
    15781 ttcctcacgg cttaacattt tcacccactt accattatgc aagtagtagc ctggtttctc
    15841 tgccgacagc cgttcagcaa aaatatcgac atcatcgccg aaacctgccg ttgctcccca
    15901 ggaaatcaca ccattatgac caaaaaccag cccaggatag gcaaatggtg tattgccagt
    15961 gacatcataa ccagcaccgt gcagaccaat accataagta tacgcaggcg cataccagcc
    16021 aaactgcgga ccatttacca tgattgcttt cgcatcctgg gctttgcttt tgccgatcac
    16081 ccacatattg ctggtcgttg gataccccgc cagaccattg gcaccaccct gtgcaaattg
    16141 tgcagcaata gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc catccgcccc
    16201 ttttgctggt cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt
    16261 ttgcgagttt tgctgattaa atttaagtgg gtagctactc tcttgtacgg caatagtggt
    16321 tggcgctgat gggtttacca gccatttcaa ctgattaaat accgccatgc cttgtgatac
    16381 accatattta tcttttaaag ccgttagcag tgccagatta tcaatttcgc tagtgctatc
    16441 agagaagcgg tttgccatgg tgcccacaaa tatcatcgcg acatcaaacg gttcccagcg
    16501 cttaggagta aagccaaatg tattaaactg ttttggtaag agcgtctctg gattggtatt
    16561 taccttatca atccaggcat tcattccatc agcgtagcct tgcagaatgg acatatcctc
    16621 tggggaaagg gcatcaattt gcgcccggat agcatccggc cagtagttac gacggatatc
    16681 tttatcaaat ttcacaaaat ctttgccaag cacttccgcg acagtccctt gagtactgcg
    16741 acgtgccatt tccatctgaa aaaggcgatc ttgtgctact acatagccat agccataaaa
    16801 taggtgccat gtatcattgg cataaatatg cggcatgccg tattcatcgc gaacaatctt
    16861 tatctcactt gacgactgct cagccagtgc aggtaagctc caataataca tcagggaagc
    16921 agtaacacag ttcacgatca tacgatttct atttttcatt gtatcctctg gcaggtgtat
    16981 aattagcgaa ttgatactag caacgaagct tcggttatct atttgcagag aattgtttca
    17041 ttatgaactg tgagcgcgat aactaatttt tgatacaaaa atttcattaa tgtgatctaa
    17101 aacgaatcgc tttattcacc gctgatgatt ttgttctatt cctttttgtg acagatcccc
    17161 cggacgcata gatgatgacc cccagagaga cagctcaggt cattatctgt atgtaaatca
    17221 gaaaaatatg ttgtaaacga cttttatggt tggattttgt agcaattttg aatgcagaca
    17281 ggcagcattt gccaacacgc cgcccatagt tcactccaca tcctccctgc gtaacccgat
    17341 atccctcaac cgttcatcac tcatctgctg taacacccgt cgggcctgca tttgccgccg
    17401 ccaacgcctc accgcctgcc agagttgcac aagtctgata aacggcgttt tggctttgtt
    17461 ttcgtgaaat tccatctgct gcctcctcat ctttcagagg cttcatcatc cgtcttctgg
    17521 cagttcacaa tacagatgca aatattagtt ttatttaaca tacagacagg ttaaaacagc
    17581 atctgcatcc ggttttttag cctcatctgt accggttttt ctctctgtat ggtccaaata
    17641 aggaatacag catgacgcgt tatcaacatc tggcaactct gcttgccgag cggattgagc
    17701 aagggctgta tcgtcacggg gagaaattgc cgtcggtgcg cagcttaagt caggagcacg
    17761 gcgtcagcat cagcaccgtg cagcaggcgt accagacgct ggagacgatg aagctcatca
    17821 ctccgcagcc gcgttcgggt tattttgtcg cacaacgtaa agcccagccg cctgtaccgc
    17881 cgatgacgcg tccggtgcag cgcccggtgg aaattaccca gtgggatcag gtgctggata
    17941 tgctggaggc gcatagcgac aattccattg ttccgttaag caaaagcacg ccggatgtcg
    18001 aaacgcccag cctgaaaccg ctgtggcgtg agctaagccg ggtggtgcag cataatctgc
    18061 aaaccgttct cggttatgac ttgttagccg gtcagcgagt gttgcgggag cagattgccc
    18121 gcctgatgct cgacagcggc tcggtggtca ccgccgatga catcatcatc accagcggct
    18181 gccataactc gatgtcgctg gcgttaatgg cggtgtgtaa accgggcgat attgtcgcgg
    18241 tcgaatctcc ctgttattac ggttcgatgc agatgctgcg aggcatgggc gtgaaagtga
    18301 ttgaaatccc aaccgatcca gaaactggca tcagcgttga agcactggaa ctggcgctgg
    18361 aacagtggcc gattaaaggc atcattctgg tgccaaactg taataatccg ctgggattta
    18421 ttatgccgga cgcacgcaaa cgggccgttc tctctctcgc tcagcgtcat gatattgtga
    18481 tttttgaaga tgatgtctac ggcgaactgg caacggagta tccgcgcccg cggaccatcc
    18541 attcatggga tatcgacggg cgagtgttgt tgtgcagctc gttcagtaaa agtattgctc
    18601 caggcctgcg cgtgggttgg gtcgcaccgg ggcgttatca cgataaactg atgcatatga
    18661 aatacgccat cagcagcttt aatgtgccgt ccacgcaaat ggcggcggca acgtttgtgc
    18721 tggaaggtca ctatcatcgc catatccggc ggatgcggca gagctatcag cgcaatttgg
    18781 cgctttatac ctgctggata cgggaatatt ttccctgcga aatctgtatt acgcgcccga
    18841 aaggcggatt tttactgtgg atagaattgc ctgaacaggt cgatatggtt tgcgttgcgc
    18901 ggcagctata ctgcatgaaa atccaggtgg cggcaggctc gattttctcg gcttccggca
    18961 aataccgtaa ttgtctgcgc atcaactgcg ctttgccgct caacgaaacc tatcgcgaag
    19021 cactgaagca aattggcgag gccgtgtatc aggcaatgga atgaattcga attagtggcg
    19081 aggcccctga ttttctctca cgactagcat gtgactcgtt ttatctttgc agggtaatcg
    19141 ccacgttcca gcatggctta ggcaaagtgc gcttcgttca tgccggatgc catgtgaacg
    19201 ccttaccagg actacaaaat ctttaaagtt caatgtattg aatgggttat gtagacctga
    19261 taagtgtagc gcatcaggct gctttgcgtt tgtcatccgt ctctgattag tactcgatcc
    19321 atggatcacg cttgctttca ggcaagggct gatctaatgc ctgcatttgc tctgccgaaa
    19381 gtccggtaat cttttgtatc cattcaggat ctgccccatt ctgcaaaagc aatctaagga
    19441 ggcgctgttc cccttcttct ttccctttct ccctccctaa cagccatcca tcattatgaa
    19501 ttcgctctgc aatcgtcatt attcgctccc tttgttgtgg cattcgacgg gtaagttcgc
    19561 tgataaactt cttaaaacgc gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag
    19621 ttatctggct gtcattagca cactctgtaa ccagtaatac taccagttga tcgataagct
    19681 ccatcagatc gcgctggcga atatgctttt ggatcaactc caacagggcg actctgcgat
    19741 gctgcacaat ctcgtcgtct ggcactacag tgacatccac cagcgggaac gctgcgctat
    19801 aaagtttccg tgcggtagtc gggtcggcaa attcgtccag ccagcacagg gaccaggggt
    19861 aaggactacg gctaccgtga taaaacagca tcgggatgac cagcggtagc ggccggcgtt
    19921 tatcatgctc gatatggcgc tgcatcaccg ccatggaata gcgcatcagg cgaaaggcca
    19981 tatgaatgtc ctcgcggctc tgatgttcaa tcaccacata gatataacca tcgccttcgc
    20041 gggtctttac cgaccacaga atatcggaat gtagcgcccg caatttttca tcgacaaagc
    20101 tggcggattc cagttttaag ctgtcgagat cgcacagttc acgtaaatct ttaggtaagt
    20161 ggatctccat aaaatcccgc gcggtgtcag ggtgcgtgag aaaggactta aataatgcgt
    20221 catgcggcgt gctggttgtg aagtttgtca tggcgatccg tcacctgcta ataagatggc
    20281 ccgactgtag cgactaacaa aaccattctc acccggcaat ttctcgttcc ccgaggcact
    20341 ttccaggcta tttttgttcg tctgcaaacg aaacattttt ccggaaacag gctcgcgttt
    20401 tacatttcct gtaccaggca gatattcccc tttccaccgc gctcttttga ttaaccgcaa
    20461 attttaatta cacttaaggt gtatattttc tatgcaaccc atcaattcaa gaggtgtaat
    20521 gtgctgatga ctatttgtaa tcgttataca ttctgacctg gagtcagaag gtatttctct
    20581 gtctgtgtgt tcacaggcag tgtggttgat tacatgaaat tcagtacatt tgtccttctc
    20641 gcctgccttt cgtcattacc gacggtattg aatttcgttt tccccgttgg ggttctccgg
    20701 acaaggagtt gtttgttatg ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc
    20761 cgatggcgtt gattttgtat gactttgccg cgtacctgtc gacggatctg atccagccgg
    20821 ggatcattaa tgtggtccgt gattttaatg ccgatgtcag tctggccccg gcagccgtca
    20881 gtctctatct cgctggcggt atggcgttac agtggctgct ggggccactt tccgacagga
    20941 ttggccgcag gccggtactg attaccgggg cgctcatttt tacccttgcc tgcgctgcga
    21001 caatgttcac cacgtctatg acacagtttc ttatcgcgcg tgcaattcag ggcaccagta
    21061 tctgttttat tgctaccgtt ggttatgtca cggtgcagga ggcgttcgga cagacaaaag
    21121 ggattaagtt gatggcgatt atcacctcca tcgtactgat tgcgccgatt attggcccgc
    21181 tttccggcgc agctctgatg cactttgtgc actggaaagt cctttttgcc atcattgcgg
    21241 ttatgggttt tatctcattt gttggcttac tgctggcgat gcctgagacg gtgaagcgcg
    21301 gcgcggttcc atttagcgcc aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc
    21361 ggctgttcct ctttggcgca acaaccatct ctttaagcta tatcccaatg atgagttggg
    21421 tggctgtctc gccggtgatc ctgattgacg caggcggctt aacaacttcg cagttcgcct
    21481 ggacacaggt tccggtgttc ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg
    21541 ttaaagatcc gaccgaaccg cgctttatct ggcgtgccgt acccattcaa ctggttggcc
    21601 tcgcgctgtt gattatcggc aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg
    21661 gcaccagttt gtatgctttc gggattggtt taatcttccc gaccttattc cgctttacgc
    21721 ttttttccaa taacttaccg aaaggaaccg tctccgcatc gctgaatatg gtgatcctga
    21781 tggtgatgtc tgtctcagtc gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc
    21841 cgtttcatct gttagccgtc gtggcgggca ttatcgtcgt tttcaccctg gcaggattgc
    21901 ttaaccgcgt gcgccaacat caggctgccg agctggcgga ggagcggtga tttttgcgcg
    21961 atcctgccgt caggctctat tcttaacgtt atgaataaac tcattgaact cagacgcgcc
    22021 aaaatgttgg cgctctcttt actgcttatc gccgctgcta cctttgtcgt tacgctgttt
    22081 ttgccgccca atttttgggt gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc
    22141 gcgctggcgg actggtttgc ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt
    22201 tctcgtcata cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa tctcggccag
    22261 ttcgtgcagg aaaaatttct cgatacccag tcgctggtgg cattgattcg acgtcacgaa
    22321 ccggcgttgt tgattggcaa ctggtttagt cagccagaaa acgcccgccg cgttggtcag
    22381 catctgttgc agatcatgag cggttttctt gaactgaccg atgatgcgcg tattcagcgc
    22441 ttgcttaagc gcgcggtcca tcgggcgatt gataaggtcg atctttccgg caccagtgcg
    22501 ttgatgctgg agagtatgac caaaaacgaa cgtcatcagg tgctgctgga tacgctgatc
    22561 gcacagttga tcgcccttct ccagcgcgat aaatcgcgca agtttatcgc ccagcaaatt
    22621 gttcgctggc tggagagcga gcatccactg aaagccaaaa ttttgcccac tgaatggctg
    22681 ggcgaacata gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga tgatatcagc
    22741 cgcgatcgtg cgcatcagat ccgtcatgcg tttgatcgcg ccacttttgc cctgatcgac
    22801 aagttgaaaa acgatccgga aatggcagcg cgagccgatg ccgtaaaaag ttatctgaaa
    22861 gaagatgaag cttttaaccg ctatctcagt gaattgtggg gggatttacg ggaatggctg
    22921 aaagcggata tcaacagtga agattctcgt gtgaaagaac gtatcgcgcg ggcgggtcaa
    22981 tggtttggcg aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa tggtcacctg
    23041 gaacaagccg cgcaccgcgt cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc
    23101 gatacagtaa aaagctggga tgcgcgggat atgtcgcggc aaatcgagtt aaatatcggc
    23161 aaagatctgc agtttatccg tgtcaacggt acgctggttg gcggttgtat tgggctaatt
    23221 ttatatttgt tgtcgcagct cccggccttg ttccccctcg gcaattttta gaaatccatg
    23281 aaaaactaag tggctaaccg cgagagagat caaataaacc acactaaaag aatggtaata
    23341 atgtggcctc ttttcattat ctcccgtggt acggggaagg aaaatcatgt cacttatcac
    23401 cgatctaccc gccattttcg atcagttctc tgaagctcgc cagaaaggct ttctcaccgt
    23461 catggatctc aaggagcgcg gcattccgct ggttggcact tactgcacct ttatgccgca
    23521 agagatcccg atggcagccg gtgcggttgt ggtttcgctc tgttctacct ctgatgaaac
    23581 cattgaagaa gcggagaaag atctgccgcg caacctctgc ccgctgatta aaagcagcta
    23641 cggcttcggc aaaaccgata aatgccccta cttctacttt tcggatctgg tggtcggtga
    23701 aaccacctgc gacggcaaaa agaaaatgta tgaatacatg gcggagttta agcccgttca
    23761 tgtgatgcag ttgccgaaca gcgttaagga cgatgcctcg cgtgcgttat ggaaagccga
    23821 gatgctgcgc ttgcaaaaaa cgatagaaga acgttttggg cacgagatta gcgaagatgc
    23881 tctgcgcgat gccattgcgc tgaaaaaccg cgaacgtcgc gcactggcca atttttatca
    23941 tcttgggcag ttaaatcctc cggcgcttag cggcagcgac attctgaaag tggtttacgg
    24001 cgcaaccttc cggttcgata aagaggcgtt gatcaatgaa ctggacgcga tgacagcccg
    24061 cgttcgtcag cagtgggaag aaggccagcg gctggccctg cgtccacgca ttttaatcac
    24121 cggctgcccg attggcggcg cagcagaaaa agtggtgcgc gcgattgaag agaatggcgg
    24181 ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg accgagcaat gcgtggcaga
    24241 aacgggcgat gtctacgacg cactggcgga taaatatctg gcgattggct gctcctgtgt
    24301 ttcgccgaac gatcagcgcc tgcaaatgct cagccagatg gtggaagaat atcaggtcga
    24361 tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat cgctggcgat
    24421 taaacgtcat gtgcgtcagc agcacaacat tccttatatc gctattgaaa cagactactc
    24481 cacctcagat gtcgggcagc tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg
    24541 agtggcagtg acatattcga ttggcattga ttctggttct accgccacca aagggatctt
    24601 actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc gtccggcaac
    24661 agcaattact gaagcctggg aaactctgcg cgaagggtta gagacaacgc cgtttctgac
    24721 gctcaccggc tatggacggc aactggtgga ttttgccgat aaacaagtga cggaaatctc
    24781 ctgtcatggg ctgggcgcac ggtttcttgc gccagcaacg cgcgcggtaa tcgacatcgg
    24841 tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg atttcctgat
    24901 gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg
    24961 caccagcgtc gagcaactcg acagcattac cgaaaatgtc acgccgcacg ccatcacgag
    25021 tatgtgcaca gtgtttgccg aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc
    25081 agaagcgatt ctcgccggag tgattaacgc gatggcgcgg aggagtgcca atttcattgc
    25141 tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagtcatt gccagacgtt
    25201 tgcccggatg ctggaatctc atctgggaat gccggtaaat acccatcctg atgcgcaatt
    25261 tgctggcgca attggcgcgg cggtaattgg tcaacgagtg aggacacgcc gatgaaagag
    25321 tttttatttt tatttcactc cacagtcggc gtcatacaaa cccgcaaagc gttgcaggca
    25381 gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta
    25441 tgcatttggc tgacctgtcc ccccggcgag gaaatacaat gggtgatccc tgggctgact
    25501 gagtcaattt attgtcagca ggatggtgtc tggcgctgca ttgcacatta ccgggtttct
    25561 ccgcgttaag gattcattaa ggtcgctacg tatacttctc gatatcttat tgatttgcct
    25621 taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatctccg ttattgtgat
    25681 tctttttgcc attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa
    25741 tcatgcagca tcatttcaga attaccacgc caccattgac ggcaaagaaa ttgcaggcat
    25801 aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt ttagcactat
    25861 caacaaacct gccgccattg ttgaaatgac caccaatggt gattttatcc gcacaattcc
    25921 tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc agtttgtcat
    25981 tagcgatgag cgcgattacg ccatttatgt gatttcactg accccaaact cggaagtaaa
    26041 aatcctcaaa aaaataaaaa tcccgctgca agactcccca accaattgcg gttttgaagg
    26101 gttggcttat tctcgccagg atcacacttt ctggtttttt aaagagaaaa atccgattga
    26161 ggtctacaaa gttaacgggc tgttaagtag caatgaatta cacatcagca aagacgaggc
    26221 actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa
    26281 tacgcttctg gtgctgtctc atgaatctcg cgcactccag gaagtaaccc tggttggcga
    26341 ggttatcggt gaaatatcac taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca
    26401 ggccgaaggg attgcgatgg atgcctctgg taatatttat atcgttagcg aaccaaatcg
    26461 tttttatcgc tttaccccta aatcatcgca ttaacaggct ggcatatttc acctttttgt
    26521 tattaacttg ttaacaacaa aaatgacata caaggttaaa catgccttcg tcctcgcatc
    26581 ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg accctaggta
    26641 tgggattagg tcgcttcctt tatacgccta tgttgcccgt catgatggcg gaaggggcgt
    26701 tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat ctggctggca
    26761 gtctgctatt ttcgtttggc gcatttcacc agccgtcgcg cctacgccca tttctgttgg
    26821 tttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgccg ccatttattc
    26881 tggtgttatt gattcgcttc ctggcgggtg tcgccagcgc cggtatgctg atttttggtt
    26941 cgacgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg ttttctggcg
    27001 ttggcattgg cattgcactg ggcaacgaat atgttctggc aggcctgcat tttgccttat
    27061 cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg attgcactta
    27121 cgcttttaat gccctcgaaa aaacacgcca tcgcaccaat gccattggca aaaacggagc
    27181 aacagataat gagctggtgg ttactggcta ttctgtacgg cctggcggga tttggttata
    27241 tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg
    27301 cccatctctg gacgttagtc ggcttatcaa ttgtgccagg ctgctttagc tggctatggg
    27361 cagcaaaacg ttggggggct ttgccttgcc tgacggcgaa tttgctggtg caggctatca
    27421 gtgtgctgct tactctcgcc agcgactctc ctctcttgct tatcattagt agtatcggtt
    27481 ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc caactgagcg
    27541 ttccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt ggacaaatcc
    27601 tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc ggcgccactc
    27661 tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtacaa ttatttaagc
    27721 tgcaagtggt cacttcatga ggagtgtttc ccttaccact caagaaactc aacagcaacc
    27781 gttgatgtca gccagacccc gttttccgct tgccagaaag ggatgcctcg tttcgccatt
    27841 tcctgtgctt tgacggttaa gataaccggc gatccgtgtc ttgctcccac tttgcgtgcc
    27901 gttgcttcat cagcagagag atgtacgtaa tgacgctccc ctgcaatcag cccctgtttt
    27961 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
    28021 tcggcaaagg aaatggcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
    28081 ccatcactgg aataactaaa acgcttttta tcgctggtgg caaccacagt atccagcaat
    28141 gcacgggtta accgcttgcc cgctttttgg gcacagagta taagcttatc gatatccgcc
    28201 catccttcac ggtcaagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
    28261 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatctc gttttcgtcc
    28321 ctggattttc tgtttttatt tactgatgtc attgtcactg ggtttatgaa ttttctgcaa
    28381 ctcaatcctg cgcagagcct tttccacaca gcataatttt tccgctatgc aaaaaacgca
    28441 tagccagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttccgctc
    28501 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
    28561 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
    28621 atctggcttt ttttctcacc ataatctttt tttccggtgt tttttccgga acagacagct
    28681 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
    28741 acggagccac cacctcattt cgcggggcgg gtggcgcggg ggctaaagat ggctttctct
    28801 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
    28861 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
    28921 gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacacg gatgccgccg
    28981 ccggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
    29041 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
    29101 cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
    29161 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
    29221 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
    29281 tggcgcgcgc cgtggcttta ctatcgccac gaccaaccta ctgccaaacg tggtgatggc
    29341 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
    29401 tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc ttttagccgc
    29461 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcgaccg ccggcacatt
    29521 aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaatgggca acccggtgca
    29581 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
    29641 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
    29701 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
    29761 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
    29821 cgttgccagc aatatccctt ctgacattgt accggactgc acggttgtcg atctcagcgg
    29881 gcagatcctc tgtcctggtt ttattgatca acacgtccat ttgattggtg gtggcggcga
    29941 agcaggtccc acgacgcgca cgccagaagt ggcgctaagt cgcctgacgg aagcgggcgt
    30001 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
    30061 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
    30121 tcatgtccct tcccgcacca ttacaggttc cgtggaaaaa gacgtggcga ttatcgatcg
    30181 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
    30241 cctggctaat atggcggcgg aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
    30301 caccgtgttc cacatgggcg acagcaaaaa ggcgttacag cctgtctatg acctgctgga
    30361 aaactgcgat gtgccgatca gcaagttgct gccgacccac gttaaccgca acgttccgtt
    30421 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
    30481 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
    30541 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
    30601 gaatttaacc catatcggtg ttgccggttt tgaaacattg ctggaaaccg tgcaggtgct
    30661 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
    30721 cggtttcctt aacctgaccg ggaaaggcga aattctgcca ggcaatgatg ctgacttgct
    30781 ggtcatgacg ccagaactgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
    30841 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
    30901 gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg gatgactgtg
    30961 gtgcgatttt gcataatatt gaaaccaaat ggctttatga ttttctgacc ctggaaaaat
    31021 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
    31081 gcatccgtgc gctggaacag gcgattggtg ttgaattgtt taaccgccag gtaacgccgc
    31141 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
    31201 tagagagcaa tctggcagag ctgcgtggcg gcagcgatta cgcgcaacga aaaatcaaga
    31261 tagccgctgc acactctctt tccctcgggc tgttaccgtc cattatcagc cagatgccgc
    31321 cgctctttac ctgggcaatt gaagctattg atgtcgatga agcggtcgat aaactgcgtg
    31381 aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
    31441 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
    31501 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
    31561 acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
    31621 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
    31681 cctggctgcc ggagtacgcc atccaacaag aaattcgcag cgggaagctc gttgtactga
    31741 atcgggacga actggtgatc ccgattcagg cctatgcata ccgaatgaat acccgcatga
    31801 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgta cttagctgac
    31861 attctccagc gtcgcactac gccaaccgcc attatcccag accagatcaa acgttagcgg
    31921 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agcgtgtgac tgtacgcttc
    31981 tttcagattg acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
    32041 ctgacggcaa taccagctct ggagatgttt gtcgctctgg ttatgcactt gccactcggg
    32101 catgcctgaa acaagtaatg acggtaatgc cgaaagagga gaattaaaaa gagtgaggag
    32161 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
    32221 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
    32281 tgccctgaac accgggcgcc actgtattga tgctcatttt ccatagccat tcccttttgt
    32341 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
    32401 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga taaaaatcat
    32461 caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttac gtagatgaaa
    32521 atacttgtca ccgccgctac ttaattccgg tgggaaatta tttatttccg cataatcatc
    32581 catgtattat ttgaaacaat cagtttgtaa ataatgtaaa ttgataacat atcaaataca
    32641 tcaaaaatac c
//
LOCUS       DAZFFI010000045        32446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFFI010000045 DAZFFI010000000
VERSION     DAZFFI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.045"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(68..1828)
                     /gene="ycaO"
                     /locus_tag="ACHJ12_002472"
     CDS             complement(68..1828)
                     /gene="ycaO"
                     /locus_tag="ACHJ12_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4990242.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPNFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1958..2650
                     /locus_tag="ACHJ12_002473"
     CDS             1958..2650
                     /locus_tag="ACHJ12_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4990243.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2849..3937
                     /gene="serC"
                     /locus_tag="ACHJ12_002474"
     CDS             2849..3937
                     /gene="serC"
                     /locus_tag="ACHJ12_002474"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4990244.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGADVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKVNRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4008..5291
                     /gene="aroA"
                     /locus_tag="ACHJ12_002475"
     CDS             4008..5291
                     /gene="aroA"
                     /locus_tag="ACHJ12_002475"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4990245.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEVENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5460..6224
                     /gene="ycaL"
                     /locus_tag="ACHJ12_002476"
     CDS             5460..6224
                     /gene="ycaL"
                     /locus_tag="ACHJ12_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4990246.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6397..7080
                     /gene="cmk"
                     /locus_tag="ACHJ12_002477"
     CDS             6397..7080
                     /gene="cmk"
                     /locus_tag="ACHJ12_002477"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4990247.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7191..8864
                     /gene="rpsA"
                     /locus_tag="ACHJ12_002478"
     CDS             7191..8864
                     /gene="rpsA"
                     /locus_tag="ACHJ12_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4990248.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9024..9308
                     /gene="ihfB"
                     /locus_tag="ACHJ12_002479"
     CDS             9024..9308
                     /gene="ihfB"
                     /locus_tag="ACHJ12_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4990249.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9515..11779
                     /locus_tag="ACHJ12_002480"
     CDS             9515..11779
                     /locus_tag="ACHJ12_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO4990250.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSVWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11816..13564
                     /gene="msbA"
                     /locus_tag="ACHJ12_002481"
     CDS             11816..13564
                     /gene="msbA"
                     /locus_tag="ACHJ12_002481"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4990251.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13561..14547
                     /gene="lpxK"
                     /locus_tag="ACHJ12_002482"
     CDS             13561..14547
                     /gene="lpxK"
                     /locus_tag="ACHJ12_002482"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4990252.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14584..15816
                     /gene="ycaQ"
                     /locus_tag="ACHJ12_002483"
     CDS             14584..15816
                     /gene="ycaQ"
                     /locus_tag="ACHJ12_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO4990253.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGTFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15868..16050
                     /gene="ycaR"
                     /locus_tag="ACHJ12_002484"
     CDS             15868..16050
                     /gene="ycaR"
                     /locus_tag="ACHJ12_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4990254.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16047..16793
                     /gene="kdsB"
                     /locus_tag="ACHJ12_002485"
     CDS             16047..16793
                     /gene="kdsB"
                     /locus_tag="ACHJ12_002485"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4990255.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16947..17840
                     /locus_tag="ACHJ12_002486"
     CDS             16947..17840
                     /locus_tag="ACHJ12_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4990256.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="ACHJ12_002487"
     CDS             complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="ACHJ12_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4990257.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18732..19517
                     /gene="cmoM"
                     /locus_tag="ACHJ12_002488"
     CDS             18732..19517
                     /gene="cmoM"
                     /locus_tag="ACHJ12_002488"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4990258.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19514..20836
                     /gene="mukF"
                     /locus_tag="ACHJ12_002489"
     CDS             19514..20836
                     /gene="mukF"
                     /locus_tag="ACHJ12_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4990259.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20817..21521
                     /gene="mukE"
                     /locus_tag="ACHJ12_002490"
     CDS             20817..21521
                     /gene="mukE"
                     /locus_tag="ACHJ12_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4990260.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21521..25981
                     /gene="mukB"
                     /locus_tag="ACHJ12_002491"
     CDS             21521..25981
                     /gene="mukB"
                     /locus_tag="ACHJ12_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4990261.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26242..28089
                     /gene="ldtD"
                     /locus_tag="ACHJ12_002492"
     CDS             26242..28089
                     /gene="ldtD"
                     /locus_tag="ACHJ12_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4990262.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQMTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28270..28818
                     /locus_tag="ACHJ12_002493"
     CDS             28270..28818
                     /locus_tag="ACHJ12_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4990263.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28845..29492
                     /gene="gloC"
                     /locus_tag="ACHJ12_002494"
     CDS             28845..29492
                     /gene="gloC"
                     /locus_tag="ACHJ12_002494"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4990264.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29715..30905)
                     /gene="aspC"
                     /locus_tag="ACHJ12_002495"
     CDS             complement(29715..30905)
                     /gene="aspC"
                     /locus_tag="ACHJ12_002495"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4990265.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31090..32178)
                     /gene="ompF"
                     /locus_tag="ACHJ12_002496"
     CDS             complement(31090..32178)
                     /gene="ompF"
                     /locus_tag="ACHJ12_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4990266.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7915 a         7858 c         8717 g         7956 t
ORIGIN      
        1 ttaaaaaaac gccccgtaat ataactcaga ctaatcatta agcctacatt gcgtaggcta
       61 tttgatttta ttttgcccag aatgctgctt tggcgcgctg cagcttttcg taggccttca
      121 acaacgactg atgtgcagca aacgcgtgca gatcgctatc taccggttgc aggccgtaaa
      181 acgccgcttc gccgctcatt gccgcactgg cggcttctac ggcatctgcg ccgtacatgc
      241 gaacaaacgc attcagatat tgcagcggct ggcgatcttc ttcctgtgcc agtaataaca
      301 acgtttgcag gcagcgataa tagttggcgc gttccggact aaatactgat gagttaaact
      361 ccatcgtcca ttcggtccag accagagcct gttccagatc gccaccagcc agcgccagca
      421 tggcttttaa ttcaccgata cgcagggtgt accaaccgtt atccgacccg gtcgccagac
      481 ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc ttcatccagt tgctcgatga
      541 ggttcaggta atcttctttt tcccactcgc tgcctggtag cgaaagaatc gtttcacgta
      601 aatggctgcc catactgtta ttcgcgagcc acagatcttc agccggataa atatcggaca
      661 tgccaggcac gataatacgg caagcataaa cgcccagatg ctcgtaatcg gcaatataaa
      721 cttctttatc ttctttgttg aagatagcca tcagtgtggc gaactcttct tccgtggtgc
      781 cggagaaatt ccagtccaca aacggataat cggcatcctg cttgaacagg tcccaggaga
      841 ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt atgttcagcg acttcttcat
      901 catcgaaggt tggcggagta aacacatcca aatctttcag gccacgacct tgcagcagct
      961 cggtcacggt acgttccagt gctacgccaa aattaggatg cgcaccgaaa gaggcaaagc
     1021 aggtaccgtt agcaggattg aacagtacca cgcaaatcac cggatactgg ccgccaagcg
     1081 aaccgtcata agcgaagatt gggaaaccct ccgcttccag cgtttcgatg gcttcaacta
     1141 ctgctgggta acgcgccagc acgtctgccg ggatctctgg caggctgatg ctttcagcaa
     1201 taatgcggtt tttcacgtag cgttcgaaaa cttcagacaa tccctgaacg cgtgcttcgt
     1261 tgcgggtatt acctgcggac ataccgttgg agacgtacag gttaccaatg atattcatcg
     1321 gaatataaac ggtctgattg tcggactgac gcgtaaacgg caggccgcaa ataccacgat
     1381 cttcgttacc ggattgtagg tcaatcagca tgctgccggt cagttcattc tccggatcat
     1441 aaaacgcgcg cagacggtca tcgagtagtc cttctggcac atcgtcattt tcggtcagtg
     1501 ggaaccattt ttcgttggga taatgcacga acggaccgtt ggcgatggtt tcgcccagcc
     1561 agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa atattcacca agtgcagaag
     1621 ccagcgccgc ttttttggtt gcgcctttac cgttggtaaa acacagtgcg cactctttgt
     1681 cgcgaatatg tacagaccag acgttaggca caggattcag ccaggaggcc tcttcaatct
     1741 gaaagccgag gtctgaaagt ttttgctgga agcgagcgat ggaatcttcc agagcggcat
     1801 ctttgccggg gataaatgtt tgcgtcatga aaatcacttt agtcgtacgg aaagcgcgca
     1861 ataatacggg ttttatctca aaggcgctat caccgccgcc atgccgatga actgttgact
     1921 atgcttttag cggataaacc acgataagag cataaaaatg aaggcgttcg atctccaccg
     1981 tatggcattt gataaagtgc cttttgattt ccttggcgaa gttgcactac gtagtcttta
     2041 tacctttgta ctggtttttc tgttcctcaa aatgaccgga agacgcggtg tgcgacagat
     2101 gtcgctgttt gaagtgttaa tcattctcac gctgggttca gcggcgggag atgtggcgtt
     2161 ttatgatgat gtgccgatgg tgccggtact tatcgtcttt atcactctgg cgttgttata
     2221 ccgcctggta atgtggttga tggcgcacag tgaaaaactg gaagatttac tggaaggtaa
     2281 gccggttgtt attattgaag atggcgagct ggcctggtcg aaactcaata actccaacat
     2341 gacggaattt gagttcttta tggagctacg attgcgtggc gtggagcagc tggggcaggt
     2401 acgtctggcg attctcgaaa ccaacgggca aatcagtgtc tatttctttg aagatgacaa
     2461 ggtgaaaccg ggtttactta ttttacccag tgattgtacg cagcgttaca aagtggtgcc
     2521 ggagtcggcg gactatgcct gtattcgttg tagtgaaatc attcatatga aagcggggga
     2581 aaaacaatta tgtccgcgct gtgcaaatcc agaatggacg aaggcaagtc gggcaaaacg
     2641 ggtgacctga cagtaaaaac atcggctttt tgctaataat ccgagagatt cttttgtgtg
     2701 atgcaagcca catttttgcc ctcaacggtt ttactcattg cgatgtgtgt cactgaatga
     2761 taaaaccgat agccacagga ataatgtatt acctgtggtc gcaatcgatt gaccgcgggt
     2821 taatagcaac gcaacgtggt gaggggaaat ggctcaaatc ttcaatttta gttctggtcc
     2881 ggcaatgcta ccggcagagg tgcttaaaca ggctcaacag gaactgcgcg actggaacgg
     2941 tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa gagttcattc aggttgcaga
     3001 ggaagccgag aaggattttc gcgatcttct taatgtcccc tccaactaca aggtattatt
     3061 ctgccatggc ggtggtcgcg gtcagtttgc tgcggtaccg ctgaatattc tcggtgataa
     3121 aaccaccgca gattatgttg atgccggtta ctgggcggca agtgccatta aagaagcgaa
     3181 aaaatactgc acgcctaatg tctttgacgc caaagtgact gttgatggtc tgcgcgcggt
     3241 taagccaatg cgtgaatggc agctctctga taatgctgct tatatgcatt attgcccgaa
     3301 tgaaaccatc gatggtatcg ccatcgacga aacgccagac ttcggcgcag atgtggtggt
     3361 cgccgctgac ttctcttcaa ccattctttc ccgtccgatt gacgtcagcc gttatggtgt
     3421 aatttacgct ggcgcgcaga aaaatatcgg cccggcaggc ctgacaatcg tcatcgttcg
     3481 tgaagatttg ctgggcaaag cgaatatcgc gtgtccgtcg attctggatt attccatcct
     3541 caacgataac gactccatgt ttaacacgcc gccgacattt gcctggtatc tgtctggtct
     3601 ggtctttaaa tggctgaaag cgaacggcgg tgtagctgca atggataaaa tcaatcagca
     3661 aaaagcagaa ctgctgtatg gggtgattga taacagcgat ttctaccgca atgacgtggc
     3721 gaaagttaac cgttcgcgga tgaacgtacc gttccagttg gcagatagtg cgcttgacaa
     3781 attgttcctt gaagagtctt ttgctgcagg ccttcatgca ctgaaaggtc accgtgtggt
     3841 cggcggaatg cgcgcgtcta tttataacgc catgccgctg gaaggcgtta aagcgctgac
     3901 agacttcatg gttgagttcg aacgccgtca cggctaatgc cgaaattttg cttaatcccc
     3961 acagccagcc tgtggggttt ttatttctgt tgttgagagt tgagttcatg gaatccctga
     4021 cgttacaacc catcgctcgt gtcgatggca ctattaatct gcccggttcc aagagcgttt
     4081 ctaaccgcgc tttattgctg gcggcattag cacacggcaa aacagtatta accaatctgc
     4141 tggatagcga tgacgtgcgc catatgctga atgcattaac agcgttaggg gtaagctata
     4201 cgctttcagc cgatcgtacg cgttgcgaaa ttatcggtaa cggcggtcca ttacacgcag
     4261 aaggtgccct ggagttgttc ctcggaaacg ccggaacggc aatgcgtccg ctggcggcag
     4321 ctctttgtct gggtagcaat gatattgtgc tgaccggtga gccgcgtatg aaagagcgcc
     4381 cgattggtca tctggtggat gcgctgcgcc tgggcggggc gaagatcact tacctggaac
     4441 aagaaaatta tccgccattg cgtttacagg gcggctttac aggtggcaat gttgacgttg
     4501 atggttccgt ttccagtcaa ttcctcaccg cactattaat gactgcgccg cttgcgccgg
     4561 aagatacagt tattcgtatt aaaggtgatc tggtttctaa accttatatc gacatcacac
     4621 tcaatctgat gaagacgttt ggtgttgaag ttgaaaatca gcactatcaa caatttgtcg
     4681 taaaaggtgg tcaatcttat cagtcaccgg gtacttattt ggtcgaaggc gatgcatctt
     4741 cggcttctta ctttctggca gcagcagcaa tcaaaggcgg cactgtaaaa gtgaccggta
     4801 ttggacgtaa cagtatgcag ggtgatattc gctttgctga tgtgctggaa aaaatgggcg
     4861 cgaccatttg ctggggcgat gattatattt cctgtacgcg tggtgaactg aacgctattg
     4921 atatggatat gaaccatatt cccgatgcgg cgatgaccat tgccaccgcg gcgttatttg
     4981 caaaaggcac caccacgctg cgcaatatct ataactggcg tgttaaagag accgatcgcc
     5041 tgtttgcgat ggcaacagaa ctgcgtaaag tcggtgcgga agtagaagag gggcacgatt
     5101 acattcgtat cactccaccg gaaaaactga attttgccga gatcgcgaca tacaatgatc
     5161 accggatggc gatgtgtttc tcgctggtgg cgttgtcaga tacaccagtg acgattcttg
     5221 atcccaaatg tacggccaaa acatttccgg attatttcga gcagttggcg cggattagcc
     5281 aggcagcctg aatgaacaac gggcaataaa tagccaaatc tttctttatc aaaacgtcgg
     5341 cacattgtcg gcgttttttt tcggaccttg tgagtcattt tgattaatgg tagcgtcgct
     5401 tgtcaatgta agttgttgat acataatatt tatatatgat taatcaacgg atgattcaca
     5461 tgaagaatac taaattactg ctggcgattg cgacctctgc agcattactg acagggtgtc
     5521 aaaataccca cggtattgat accaatatgg ctatcagctc cggtttaaat gcctataaag
     5581 cagcaacatt aagcgatgcc gatgcaaaag cgattgccaa tcagggctgt gccgaaatgg
     5641 acagcggcaa tcaagtcgca agtaaatcca gcaagtacgg taaacgtctg gcaaaaatcg
     5701 ccaaagcatt gggtaacaat attaatggca cgccggtcaa ctataaggtt tatatgacca
     5761 gcgacgtcaa cgcatgggcg atggcgaacg gctgtgtccg tgtctacagt ggcctgatgg
     5821 acatgatgaa tgacaacgaa attgaaggtg ttctgggcca tgaactgggc cacgtcgcgt
     5881 tgggtcactc gctggctgaa atgaaagctt cttatgcgat cgttgccgca cgcgatgcca
     5941 tttcagctac cagcggagtg gcttcccagc tttcccgctc acaattgggt gatatcgcag
     6001 aaggcgctat taatgctaaa tactcccgtg ataaagagtc cgaagcagat gatttctcct
     6061 ttgatctgtt gaagaaacgt ggcatcagca cccaggggct ggttggcagc tttgaaaaac
     6121 tggctagtct ggatggcggt cgcacccagt ccatgtttga ctctcaccca ccatcaacag
     6181 agcgtgcgca acacatccgt gatcgtatcg cctctggtaa gtaaatcatt gtcatctttc
     6241 gggctggtct tctgccagcc cgctataatt gcgcaataaa tccccatctg aatacagaca
     6301 aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc ggcgcgtata atgcgcgcgg
     6361 ttatgttaac ggtacgcctg ttttaaggag ataaagatga cggcaattgc cccggttatt
     6421 accattgatg gcccaagcgg tgcagggaaa ggcaccttgt gtaaggctat ggcggaagca
     6481 ttgcaatggc atctgctgga ttcgggtgca atttatcgcg tactggcatt ggcggcatta
     6541 catcaccatg ttgatgttgc gtcagaagat gcgctggtac cgctggcatc ccatctggat
     6601 gtacgttttg tgtcaaccaa tggcaatctg gaagtgatcc tcgaagggga agatgtcagc
     6661 ggcgaaattc gtactcagga agtggcgaat gcagcttcac aagtcgcggc attcccacgc
     6721 gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg aattaccagg cctgattgcc
     6781 gatggccgcg acatgggaac ggtggtattc cctgatgcgc cagtgaaaat tttccttgac
     6841 gcctcctcgg aagaacgagc gcatcgccgc atgctacagt tgcaggagaa gggctttagt
     6901 gttaactttg agcgcctttt ggccgagatc aaagaacgcg acgaccgcga tcgtaaccga
     6961 gcggtagcgc cactggttcc ggcagccgat gctttagtgt tggattccac caccttaagc
     7021 attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc agaaattggc tctcgcataa
     7081 gcgaccgaat ttgcagtacc cccgttgcaa tggaatggca gcgggtatgt taaacaaccc
     7141 catccggcat ggagccaggt ggacgttaaa tataaacctg aagattaaac atgactgaat
     7201 cttttgctca actctttgaa gagtccttaa aagaaatcga aacccgcccg ggttctatcg
     7261 ttcgtggcgt tgttgttgct atcgacaaag acgtagtact ggttgacgct ggtctgaaat
     7321 ctgagtccgc catcccggct gagcagttca aaaacgccca gggcgagctg gaaatccagg
     7381 taggtgacga agttgacgtt gctctggacg cagtagaaga cggcttcggt gaaactctgc
     7441 tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac gctggaaaaa gcttacgaag
     7501 atgctgaaac tgttaccggt gttatcaacg gcaaagttaa gggcggcttc actgttgagc
     7561 tgaacggtat tcgtgcgttc ctgccaggtt ctctggtaga cgttcgtccg gtgcgtgaca
     7621 ctctgcacct ggaaggcaaa gagcttgaat tcaaagtaat caagctggat cagaagcgca
     7681 acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga aaacagcgca gagcgcgatc
     7741 agctgctgga aaacctgcag gaaggcatgg aagttaaagg tatcgttaag aacctcactg
     7801 actacggtgc attcgttgat ctgggcggcg ttgacggcct gctgcacatc actgacatgg
     7861 cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt gggcgacgaa atcactgtta
     7921 aagtgctgaa gttcgaccgc gaacgtaccc gtgtatctct gggcctgaaa cagctgggcg
     7981 aagatccgtg ggtagctatc gctaaacgtt atccggaagg taccaaactg actggtcgcg
     8041 tgaccaacct gaccgactac ggctgcttcg ttgaaatcga agaaggcgtt gaaggcctgg
     8101 tacacgtttc cgaaatggat tggaccaaca aaaacatcca cccgtccaaa gttgttaacg
     8161 ttggcgatgt agtggaagtt atggttctgg atatcgacga agaacgtcgt cgtatctccc
     8221 tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt cgcggaaacc cacaacaagg
     8281 gcgaccgtgt tgaaggtaaa atcaagtcta tcactgactt cggtatcttc atcggcctgg
     8341 acggcggcat cgacggcctg gttcacctgt ctgacatctc ctggaacgtt gcaggcgaag
     8401 aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc agttgttctg caggttgacg
     8461 cagaacgtga acgtatctcc ctgggcgtta aacagctcgc agaagatcca ttcaacaact
     8521 gggttgctct gaacaagaaa ggcgctatcg taaccggtaa agtaactgca gttgacgcta
     8581 aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta cctgcgtgct tctgaagcat
     8641 cccgtgaccg cgttgaagac gctaccctgg ttctgagtgt tggcgacgaa gttgaagcta
     8701 aattcaccgg cgttgatcgt aaaaaccgcg caatcagcct gtctgttcgt gcgaaagacg
     8761 aagctgacga gaaagatgca atcgcaactg ttaacaaaca ggaagatgca aacttctcca
     8821 acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga gtaattctct gactcttcgg
     8881 gatttttatt ccgaagtttg ttgagtttac ttgacagatt gcaggtttcg tcctgtaatc
     8941 aagcactaag ggcggctacg gccgccctta atcaatgcag caacagcagc cgcttaattt
     9001 gcctttaagg aaccggagga atcatgacca agtcagaatt gatagaaaga cttgccaccc
     9061 agcaatcgca cattcccgcc aagacggttg aagatgcagt aaaagagatg ctggagcata
     9121 tggcctcgac tcttgcgcag ggcgagcgta ttgaaatccg cggtttcggc agtttctctt
     9181 tgcactaccg cgcaccacgt accggacgta atccgaagac tggcgataaa gtagaactgg
     9241 aaggaaaata cgttcctcac tttaaacctg gtaaagaact gcgcgatcgc gccaatattt
     9301 acggttaagt ttttactcaa acttgaacgc gagaaaagca cctgtcgggt gcttttttca
     9361 tttctctaat caggaactgg aagctgcctc gcagagtttt gagcagtttt caccctttcg
     9421 ttaaattctt ctgaatgtgc ctcggggaac gcaaaattcc caccagacaa ccgctcaaca
     9481 aagttgcaca ctttccataa acagggaggg gtgcatgaaa ataacgacag tcggcgtatg
     9541 cataatttgc ggaatttttc cgttgctgat tttgccccaa ttgcctggga cattaaccct
     9601 tgcgtttctg actctcttcg cctgcgtact ggcatttatc cctgttaaaa ccgtccgtta
     9661 tatcgcgctg acgttgctgt ttttcgtttg gggcatatta tcagcaaagc aaattttgtg
     9721 ggcaggagaa accttaactg gcgcgacgca ggatgcaatt gttgagatca ctgcaactga
     9781 cggcatgacc actcattacg gtcaaattac tcatctacaa ggtcgacgta tattccctgc
     9841 gccaggcctt gtgctgtatg gcgaatatct tccgcaagcg gtttgtgctg gacaacaatg
     9901 gtcaatgaaa ctcaaagttc gtgcggttca tggccaactt aatgatggcg gctttgatag
     9961 ccagcgttat gccattgccc agcatcaacc gctcaccggc cgctttctgc aggcaagtgt
    10021 tattgaaccg aattgtagcc tgcgtgcaca gtatctggcg tcactacaaa caacgctgca
    10081 accctatccg tggaatgcgg ttattcttgg tttaggtatg ggggaacggt tatccgtccc
    10141 caaagaaatc aaaaatatca tgcgcgatac tggaacggcg catttaatgg cgatatcggg
    10201 attgcacatc gcttttgcgg cgttgctggc tgccggactc attcgcagtg gacaaatttt
    10261 tctgcctggg cgctggatcc actggcaaat accattaatt ggtgggatct gctgtgctgc
    10321 tttttatgcc tggttgacgg gaatgcaacc tcctgcattg cgtaccgtgg tggcgcttgc
    10381 tacgtgggga atgcttaagt taagtgggcg acagtggagt gtctgggatg tatggatatg
    10441 ttgtctggcg gcaattttgc tgatggatcc tgttgccatt ctctcgcaaa gtttatggct
    10501 ctctgccgct gcggtcgcgg cactgatttt ttggtaccag tggtttccct gtcctgagtg
    10561 gcaactgccg ccggtattgc gtgcagttgt ttccctcatt catttgcaac tgggaatcac
    10621 actcctgctt atgcctgtgc aaatcgtcat atttcatggc attagtctga cctcgtttat
    10681 tgcaaatcta ttagcaattc ccttggtgac atttatcacg gttccgttga tcctcgccgc
    10741 gatggttgtg catttaagcg ggccgttaat cctggagcaa gggttatggt ttcttgccga
    10801 ccggtctttg gctttacttt tctgggggtt aaagagtttg ccagaagggt ggatcaacat
    10861 tgctgaacgt tggcaatggc tatcattttc cccatggttc ttactggtgg tatggcgatt
    10921 aaacgcctgg cgaacgttgc cagcaatgtg tgtggctgta ggcttgctga tgtgctggcc
    10981 gctgtggcaa aaacctcgac ctgacgagtg gcaggtgtac atgcttgatg tcgggcaagg
    11041 gctggcaatg gtgatagcca gaaacggcaa agcgattctc tatgacacag gactggcctg
    11101 gcccgaaggg gatagtgggc aacaactgat tatcccctgg ctccactggc ataatcttga
    11161 accggaaggc gttattctga gtcatgaaca tctggatcac cggggagggc tggactcaat
    11221 attgcacaca tggccgatgt tatggatcag aagtccgtta aactgggaac atcatcagcc
    11281 ctgtgtgcgt ggcgaagcgt ggcaatggca aggattgcgt ttcagcgtgc actggccttt
    11341 acaagctagc aacgataaag gaaataacca ttcctgtgtg gttaaggttg atgacgggac
    11401 gaatagcatt cttctaaccg gtgatattga agtccccgct gaacaaaaga tgctaagccg
    11461 ttactggcag caagtgcaga caacattgct tcaggtacct caccatggca gtaatacctc
    11521 atcatcgttg ccattaattc agcgagtgaa tggaaaagtg gcactcgcat cggcatcgcg
    11581 ctataacgca tggcgattgc cctctaataa agttaagcat cgctatcaac agcaaggata
    11641 tcaatggctt gatactccac atcagggtca agtgacggtc aatttttcag cgcaaggctg
    11701 gcggattagc agcctcagag agcaaatttt acctcgttgg tatcatcagt ggtttggcgt
    11761 gccagtggat aacgggtaga atatgcggct atttcaacaa atgctggttt tttgaatgca
    11821 taacgacaaa gatctctcta cgtggcagac gttccgccga ctgtggccaa ccattgcgcc
    11881 ttttaaagcg ggtctgatcg tggcgggcgt agcgttaatc ctcaacgcag ccagcgatac
    11941 cttcatgtta tcgctcctta agccacttct tgatgatggc tttggtaaaa cagatcgctc
    12001 cgtgctggtg tggatgccgc tggtggtgat cgggctgatg attttacgtg gtatcaccag
    12061 ctatgtctcc agctactgta tctcctgggt atcaggaaag gtggtaatga ccatgcgtcg
    12121 ccgcctgttt ggtcacatga tgggaatgcc ggtttcattt tttgacaaac agtcaacggg
    12181 gacgctgttg tcacgtatca cctacgattc cgaacaggtt gcttcttctt cttccggcgc
    12241 actgataacg gttgtgcgtg aaggtgcgtc aatcatcggc ctgttcatca tgatgttcta
    12301 ttacagttgg caactgtcga tcattttgat tgtgctggca ccgattgttt cgattgcgat
    12361 tcgcgtagta tcgaagcgtt ttcgcaacat cagtaaaaac atgcagaaca ccatggggca
    12421 ggtgaccacc agcgccgaac aaatgctgaa gggccacaaa gaagtattga ttttcggtgg
    12481 tcaggaagtg gaaacgaaac gctttgataa agtcagcaac cgaatgcgtc ttcaggggat
    12541 gaaaatggtt tcagcctctt ccatctctga tccgatcatt cagctgatcg cctctttggc
    12601 gctggcgttt gttctgtatg cggcgagctt cccaagtgtc atggatagcc tgactgccgg
    12661 tacgattacc gttgttttct cttcaatgat tgcactgatg cgtccgctga aatcgctgac
    12721 caacgttaac gcccagttcc agcgcggtat ggcggcttgt cagacgctgt ttaccattct
    12781 ggacagtgag caggagaaag atgaaggtaa gcgcgtgatc gagcgtgcga ctggcgacgt
    12841 ggaattccgc aatgtcacct ttacttatcc gggacgtgac gtacctgcat tgcgtaacat
    12901 caacctgaaa attccggcag ggaagacggt tgctctggtt ggacgctctg gttcgggtaa
    12961 atcaaccatc gccagcctga tcacgcgttt ttacgatatt gatgaaggcg aaatcctgat
    13021 ggatggtcac gatctgcgcg agtataccct ggcgtcgtta cgtaaccagg ttgctctggt
    13081 gtcgcagaat gtccatctgt ttaacgatac ggttgctaac aacattgctt acgcacggac
    13141 tgaacagtac agccgtgagc aaattgaaga agcggcgcgt atggcctacg ccatggactt
    13201 catcaataag atggataacg gtctcgatac agtgattggt gaaaacggcg tgctgctctc
    13261 tggcggtcag cgtcagcgta ttgctatcgc tcgagccttg ctgcgtgata gcccgattct
    13321 gattctggat gaagctacct cggctctgga taccgaatcc gaacgtgcga ttcaggcggc
    13381 actggatgag ttgcagaaaa accgtacctc tctggtgatt gcccaccgct tgtctaccat
    13441 tgaaaaggca gacgaaatcg tggtcgtcga ggatggtgtc attgtggaac gcggtacgca
    13501 taacgatttg cttgagcacc gcggcgttta cgcgcaactt cacaaaatgc agtttggcca
    13561 atgatcgaaa aaatctggtc tggtgaatcc cctttgtggc ggctattgct gccactctcc
    13621 tggttgtatg gcctggtaag tggcgcgatc cgtctttgct ataaactaaa actgaagcgc
    13681 gcctggcgtg cccccgtacc ggttgtcgtg gttggtaatc tcaccgcagg cggcaacgga
    13741 aaaaccccgg tcgttgtctg gctggtggaa cagttgcaac agcgcggtat tcgcgtgggg
    13801 gtcgtatcgc ggggatatgg tggtaaggct gaatcttatc cgctgttatt gtcggcagat
    13861 accacaacag cacaggcggg tgatgaacct gtgttgattt atcaacgcac tgatgcgcct
    13921 gttgcggttt ctcccgttcg ttctgatgcg gtaaaagcca ttctggcgca acaccctgat
    13981 gtgcagatca tcgtaaccga cgacggttta cagcattacc gtctggcgcg tgatgtggaa
    14041 attgtcgtta ttgatggtgt gcgtcgcttt ggcaatggct ggtggttgcc ggcggggcca
    14101 atgcgtgagc gagcggggcg cttaaagtcg gttgatgcgg taatcgtcaa cggtggtgtc
    14161 cctcgcagcg gtgaaatccc catgcatctg ctgccgggtc aggcggtgaa tttacgtacc
    14221 ggtacgcgtt gtgacgttgc tcagcttgaa catgtagtgg cgatggcggg gattgggcat
    14281 ccgccgcgct tttttgccac gctgaagatg tgtggcgtac aaccggaaaa atgtgtaccg
    14341 ctggccgatc atcagtcttt gaaccatgcg gatgtcagtg cgttggtaag cgccgggcaa
    14401 acgctggtaa tgactgaaaa agatgcggtg aaatgccggg cctttgcaga agaaaattgg
    14461 tggtatttgc ctgtagacgc acagctttca ggtgatgaac cagcgaaact gcttacgcaa
    14521 ctaacctcgc tggcttctgg caactagtta cgccgcggca gcgttcgatt gatggagtca
    14581 tgaatgtcgc tgccgcacct ctcccttgct gatgcgcgta atcttcacct tgccgcacaa
    14641 ggcctgttaa acaaaccccg ccgtcaagcg tcgttggagg atattccggc aacgatctcc
    14701 cgcatgtcct tgctgcaaat cgataccatc aatattgttg cccgtagtcc atatctggtg
    14761 cttttcagtc gtctgggaaa ttatcctgcc cagtggctgg atgagtctct ggcgcgtggc
    14821 gaattaatgg aatactgggc gcatgaagcc tgctttatgc cgcgtagcga ctttcgtctt
    14881 attcgccacc gcatgctggc acctgaaaaa atgggctgga aatacaaaga cgcctggatg
    14941 caggaacatg aggcggaaat tgcacagtta attcagcata ttcatgataa ggggccggta
    15001 cgttcagccg attttgagca tcctcgtaaa ggtgcaagcg gctggtggga atggaagccg
    15061 cataaacggc atctggaagg tttatttact gccggaaagg tgatggtgat tgaacggcgc
    15121 aacttccagc gcgtttatga tttaacccac cgtgtcatgc ctgactggga tgatgagcgc
    15181 gatctcgttt cgcaaacaga agcagaaatc atcatgctgg ataacagtgc gcgtagcctg
    15241 ggaacattcc gcgaacagtg gctggcagat tactatcggc tgaaacgtcc ggcactggcg
    15301 gcgtggcgcg aagcgagggc tgaacagcag caaatcattg ctgtgcatgt tgaaaaattg
    15361 ggcaatcttt ggctgcatgc tgatttgctg ccgctactcg agcgtgcgct ggccggaaag
    15421 ctcactgcaa cgcacagcgc ggtactttcg ccttttgatc ctgttgtctg ggatcgcaaa
    15481 cgcgcagagc agctttttga ttttagctac cggctggagt gctatacccc agcgccgaaa
    15541 cgccagtatg gctattttgt tctgccatta ttacatcgtg ggcaattagt tgggcgaatg
    15601 gatgccaaaa tgcatcgcca gacaggcatc cttgaagtta tctctctgtg gttacaggaa
    15661 ggcattaaac caacgacaat gctgcaaaaa gggttacgtc aggcgattac tgatttcgcg
    15721 agctggcagc aggcaacgcg ggtgacatta ggacgctgcc cgcaaggtct ctttactgat
    15781 tgccgcactg gctgggaaat agaccccgtc gcataaatga ttatgataag atttatgcat
    15841 tcataacccg atccatctgg aggaactatg gatcatcgtc tgcttgaaat cattgcctgc
    15901 ccggtttgca acggaaaact ttggtataac caagaaaaac aagaacttat ttgcaaactg
    15961 gacaacctgg ctttcccgct gcgtgatggc attccagtgt tgctggaaac ggaagcccgc
    16021 gtgctgactg ctgatgagag taaatcatga gttttgtggt cattattccc gcgcgctacg
    16081 cgtcaacgcg tctgcccggt aaaccattgg ttgatattaa tggcaaaccc atgattgttc
    16141 atgttcttga acgtgctcgc gaatcaggtg ccgatcgcat catcgtggca accgatcatg
    16201 aggatgtcgc ccgcgccgtt gaagccgctg gcggtgaagt atgtatgacg cgcgccgatc
    16261 accagtcagg gacagaacgc ctggcggaag ttgtcgaaaa atgcgcattc agcgacgaca
    16321 cggtgatcgt taatgtgcag ggtgatgaac cgatgatccc tgcgacaatc attcgtcagg
    16381 ttgctgataa cctcgctcag cgtcaggtgg gtatggcgac tctggcggtg ccaatccaca
    16441 atgcggaaga agcgtttaac ccgaatgcgg tgaaagtggt tctcgacgct gaagggtatg
    16501 cactgtactt ctctcgcgcc accattcctt gggatcgtga tcgttttgca gaaggccttg
    16561 aaaccgttgg cgataacttc ctgcgtcatc ttggtattta tggctaccgt gcaggcttta
    16621 tccgtcgtta cgtcaactgg cagccaagtc cgttagaaca catcgaaatg ttagagcagc
    16681 ttcgtgttct gtggtacggc gaaaaaatcc atgttgctgt tgctcaggaa gttcctggca
    16741 caggtgtgga tacccctgaa gatcttgagc gcgttcgcgc tgaaatgcgc taatttcact
    16801 tcacgacact tcagccaatt ttgggaggag tgtcgtaccg ttacgatttt cctcaatttt
    16861 tcttttcaac aattgatctc attcaggtga catcttttat attggcgctc attatgaaag
    16921 cagtagcttt tatgagggta atctgaatgg aacagctgcg tgccgaatta agccatttac
    16981 tgggcgaaaa actcagtcgt attgagtgcg tcaatgaaaa agcggatacg gcgttgtggg
    17041 ctttgtatga cagccaggga aacccaatgc cgttaatggc aagaagcttt agtacgcccg
    17101 gaaaagcccg acaactggca tggaaaacca ccatgctggc aagaagtggg actgtccgta
    17161 tgccgactat ttatggtgtg atgacgcatg aagaacaccc cggccccgat gtcctgctac
    17221 tggagcggat gcgtggtgtt tcggtggagg caccagcccg aacaccagaa cgctgggaac
    17281 aactcaaaga ccaaatcgtt gaagccttac tggcctggca ccgtcaggac agtcgcggtt
    17341 gcgtcggcgc ggtcgacaat actcaggaaa atttctggcc ctcatggtac cggcaacatg
    17401 ttgaagtgct atggaccacg ctcaatcagt tcaataacac cggtctgacg atgcaggata
    17461 agcggatcct gtttcgcact cgcgaatgtc tcccggcatt atttgaaggc tttaacgaca
    17521 attgtgtgct gattcacggt aacttctgtt tacgcagcat gttgaaagat tcgcgcagcg
    17581 atcagttact ggcgatggtc gggccgggac taatgctttg ggcaccgcga gaatacgaac
    17641 tgttccgact aatggataat tctctggcgg aagatttgct ctggagttac ctgcaacgcg
    17701 cgccagtggc ggagtcgttc atctggcggc gttggttgta tgtgttatgg gatgaagttg
    17761 cgcaactggt taataccgga cgatttagtc ggcgcaactt cgatctggca tcaaaatcac
    17821 tcttgccgtg gctcgcctga cgaacctttt agccactgcc agatacggcc aagcgtttca
    17881 tagccaacac gatcgctatg catcaaccag accggagaag ggattgcccg ttcccacggg
    17941 ttaagcggcg agtcgatggc caactgattc gccggggctg gtagcggatt taacccttcc
    18001 tgctgaaaaa agatcatcgc ccgcggcaga tgtgatgcgg aggtcaccag caggaaaggg
    18061 gcgtcaccaa tcgcctgttt cactgccgca gcttcttctt cggtatcttt tggcaaatcc
    18121 agggtgataa tttgctcgtg cggcacgccc agcgattgcg caactctggc acctacttcc
    18181 gctgtactca ccgtattggt ttttgctacg cctcccgtga agatcagttt tgatcccgga
    18241 ttttcgcgcc ataagcgaat accttcattc aggcgtggca gactgttatt gattaaatta
    18301 gagctcggtg cccactgtgg gttccaggta taaccacctc cgagcaccac gatatagtcc
    18361 actttttgcg aattattcca tgtcggatag gtgctttcga tgggacgcag caggcggtcg
    18421 gctaccggtt gcaaactcaa taacaagagt gccagccacc caatgctaat aaaaatcttc
    18481 ccggtttttt gaaaacggct aaaccacagc agcgccaggc cagcgccaat aattaacaac
    18541 atcagcggaa gtggcagcaa catattgcca atcacttttt tcagtgtaaa aagcatcctt
    18601 tctggttcct tttttaacca tatagcaagg gatcgccagc gatattacac cagacgggtt
    18661 cattctcgcc gtggctgtga caaaatagcg tctttatcag aaaacgtctt agccaaccgg
    18721 tggagaaagc aatgcaggat cgcaattttg atgatattgc ggaaaagttt tcccgtaaca
    18781 tttacggcac caccaaaggg cagcttcgac aggctattct gtggcaggat ctcgatcacg
    18841 tgctggcgga aatgggcccg caaaaactgc gtgtgctgga tgctggcggt ggtgaagggc
    18901 agaccgcaat caaaatggcc gagcgtgggc atcaggtcat tttatgcgat ctttctgcgc
    18961 agatgatcga ccgcgcaaaa caggcggcag aagcaaaagg tgtgagcgac aacatgcaat
    19021 ttatacattg cgccgctcag gatgttgctt cgcatttgga aacgcccgtt gatctgatat
    19081 tgttccatgc ggtgctcgag tgggtggccg atccccgcag tgtattgcag accctgtggt
    19141 cagtgttgcg tccaggcggc gtgttgtcgt taatgttcta caatgcgcat ggtttgttga
    19201 tgcataacat ggtcgccggg aattttgatt acgtgcaggc gggaatgccg aaaaagaaaa
    19261 aacggacgct ttcgccagat tatccacgcg acccggcgca ggtttatctg tggctggaag
    19321 aagctggttg gcaaattatg ggtaagacag gcgttcgcgt gtttcatgat tatctgcgcg
    19381 agaagcacca gcagcgcgac tgctatgaag cattacttga attagaaacg cgttattgcc
    19441 gtcaggaacc gtatattacc ctggggcgtt atattcatgt caccgcgcgc aaaccgcaga
    19501 gcaaggataa agtatgagtg aattttccca gacagtcccc gaactggttg cctgggccag
    19561 aaaaaatgac ttctccatct cgctgccggt agaccgactc tcttttctgc tggcggttgc
    19621 cacgctgaac ggcgagcgtc tggatggtga gatgagtgaa ggcgagctgg tggatgcatt
    19681 ccgccatgtg agtgatgcgt ttgagcaaac cagcgaaacc atcggcgtgc gcgccaataa
    19741 cgcgatcaac gacatggtgc gtcaacgtct gctgaaccgc tttaccagcg agcaggcgga
    19801 agggaacgca atttaccgtc tgacgccgct cggcatcggc attactgact actacatccg
    19861 tcagcgcgag ttttctacgc tgcgtctttc tatgcagttg tcgattgtgg cgggtgagct
    19921 caaacgcgca gcagatgccg ccgaagaggg cggtgatgaa tttcactggc accgtaatgt
    19981 ctatgcgcca ctgaaatatt cggtagcaga aattttcgac agtatcgacc tgacgcaacg
    20041 tctgatggac gaacagcagc agcaggtgaa ggacgatatc gcccagttgc tgaacaaaga
    20101 ctggcgggcg gcgatttcca gctgtgaatt gttgctttcg gaaacttccg ggacgctgcg
    20161 tgaattgcag gatacgctgg aagcggcagg cgacaaattg caggctaatc tgttgcgcat
    20221 tcaggatgcg acgatgaccc atgacgatct gcatttcgtc gatcgtctgg tgttcgatct
    20281 gcagagcaaa ctcgatcgta ttatcagttg gggccagcaa tccatcgact tgtggattgg
    20341 ctacgaccgc cacgtacaca aatttattcg taccgcgatc gatatggata aaaaccgcgt
    20401 ctttgctcag cggttacgtc agtcggtaca aacctatttt gatgagccgt gggcgctaac
    20461 ttatgccaat gccgatcgtc tgctggatat gcgtgacgaa gagatggcac tgcgcgatga
    20521 agaagtgact ggggaacttc ctgaggatct ggaatacgaa gagtttaacg agatccgcga
    20581 acagctggcg gcgatcatcg aagaacaact tgccgtgtac aaaaccagac aagtgccgct
    20641 ggatcttggt ctggtggtac gcgaatatct gtcacagtat ccgcgtgcac gtcactttga
    20701 cgttgcgcgt attgttattg atcaggcggt acgtcttggc gtagcgcaag cagatttcac
    20761 cggactgcca gcgaaatggc agccgattaa tgattacgga gccaaggtac aggcgcatgt
    20821 catcgacaaa tattgaacaa gtgatgccgg ttaagctggc gcaggcgctg gcgaatccgt
    20881 tatttccggc gctggatagc gccttacgtt caggccgcca tattggcctc gacgaactgg
    20941 ataatcatgc attcctgatg gattttcagg aatatctgga agagttttac gcgcgttata
    21001 acgttgagct tattcgcgca ccggaagggt tcttctattt acgcccacgt tccaccactc
    21061 ttatcccgcg ttccgtcttg tcggaactgg atatgatggt cgggaaaatc ctctgttatc
    21121 tctatctcag cccggaacgg ctggcgaatg aggggatttt tactcagcag gaactgtacg
    21181 acgaactgct caccctggcc gatgaagcaa aactgctgaa actggtgaac aaccgttcaa
    21241 ccggttcaga cgttgaccgt cagaagttgc aggagaaagt acgttcttcg ctcaaccgtc
    21301 tgcgtcgttt aggcatggtg tggtttatgg gccacgacag cagcaagttt cgcattaccg
    21361 aatcggtgtt ccgcttcgga gccgatgtgc gtgctggcga cgatccccgt gaagcacagc
    21421 gtcgcctgat tcgtgatggc gaagcaatgc cgattgaaaa tcatctgcaa ctcaacgatg
    21481 aaaccgaaga gaatcagcca gatagcggag aggaagaata atgattgaac gcggtaaatt
    21541 tcgctcactg acgctgatta actggaacgg cttttttgcc cgaacttttg accttgacga
    21601 gctggtcacg acgctttctg gcggtaacgg ggcgggtaaa tccaccacca tggcggcgtt
    21661 cgttacggcg ctgatccccg acctgaccct gctgcatttc cgtaacacta cggaagccgg
    21721 ggccaccagc ggttcgcgcg ataaaggtct gcacggtaag ctgaaagcgg gtgtctgtta
    21781 ttcgatgctc gacaccatta actcgcgcca ccagcgcgtg gtggtcggtg tgcgtctgca
    21841 acaggttgcc ggacgcgatc gtaaagtgga tatcaagccg tttgccattc agggactgcc
    21901 gatgtcggtg cagccgacac agctggtgac cgaaaccctg aacgaacgcc aggcgcgcgt
    21961 gctgccgctt aacgagctga aagacaagct cgaggcgatg gaaggcgtgc agtttaaaca
    22021 gttcaactcc attactgatt accactcgct gatgttcgat ctgggcatca tcgcgcgtcg
    22081 tctgcgctcc gcatctgacc gtagcaaatt ctatcgtctg atcgaagctt cgctgtatgg
    22141 cgggatctcc agtgccatta cccgttctct gcgcgactac ctgttgccgg aaaacagcgg
    22201 cgtgcgtaaa gcgttccagg acatggaagc ggcgctgcgt gaaaaccgta tgacgctgga
    22261 agcgattcgt gtcacccagt cggaccgtga cctgtttaag catctgatca gcgaagctac
    22321 caactacgtg gcggcggact acatgcgtca cgccaacgag cgccgtgtcc atctcgacaa
    22381 agccctggag tttcgtcgcg agttacatac ttcgcgtcag caactggcgg ctgagcagta
    22441 caaacacgtc gatatggcgc gtgagctggc agagcacaac ggtgccgaag gcgatctgga
    22501 agcggattat caggcggcca gcgatcacct gaacctggtg caaaccgcac tgcgtcagca
    22561 ggagaagatc gaacgctacg aagcggatct cgatgagctg cagatccgtc tggaagagca
    22621 aaacgaagtg gtggcagaag ccatcgaacg ccaggaagag aacgaggctc gcgcggaagc
    22681 tgccgaactg gaagtggacg agctgaaaag ccagcttgct gactaccagc aggcgctgga
    22741 cgtccagcaa acgcgcgcga tccagtataa ccaggcgatt gctgcgctta atcgcgccaa
    22801 agaactgtgc catctgccgg acttaaccgc cgacagcgcc gccgaatggc tggaaacctt
    22861 ccaggcgaaa gagctggaag cgactgaaaa aatgctctct cttgagcaga aaatgagcat
    22921 ggcgcaaacc gcgcacagcc agtttgagca ggcttatcag ctggtggtgg caatcaacgg
    22981 cccgctggcg cgtaacgagg cgtgggatgt cgctcgcgaa ctattgcgcg aaggggtcga
    23041 tcagcgtcac ctggcagagc aggttcaacc gttgcggatg cgcttaagtg aactggaaca
    23101 gcgtctgcgc gagcagcaag aagccgaacg cctgctggca gacttctgca aacgtcaggg
    23161 caagaatttt gatattgatg aactggaagc tctgcatcag gagctggaag cgcgcattgc
    23221 ctctctttcc gatagcgtgt ctaacgcccg tgaagagcgc atggcactgc gccaggaaca
    23281 ggaacagctg cagtctcgca ttcagagttt gatgcagcgt gcgccggttt ggctggcagc
    23341 gcaaaacagt ctcaaccagt tgagcgagca gtgcggtgaa gagtttacct ccagccagga
    23401 cgtcaccgaa tatctgcaac agttgctgga gcgtgagcga gaggcgattg ttgaacgcga
    23461 tgaagtgggc gcgcgcaaaa acgctgtcga tgaagagatc gaacgtttaa gccagcctgg
    23521 cggctctgaa gatcagcgtc tgaacgcgct ggcggagcgt tttggcggcg tgctgctgtc
    23581 agaaatttat gacgacgtta gcctggaaga tgcgccgtac ttctcagcgc tgtatggccc
    23641 gtcacgccac gccatcgtgg tgccagatct gtcacaggta actgaacacc tggaaggctt
    23701 gaccgattgc ccggaagatc tctatttgat cgaaggggat ccgcagtcat tcgatgacag
    23761 cgtgttcagc gttgatgagc tggaaaaagc ggtagtggtg aaaatcgccg atcgtcagtg
    23821 gcgttattca cgtttcccgg aagtgccgct gtttggtcgt gctgcgcgtg aaagccgtat
    23881 tgaaagcctc catgccgagc gtgaagtgct ttccgaacgc ttcgccacgc tctcctttga
    23941 tgtacagaaa actcagcgtc tgcatcaggc gttcagccgc tttatcggca gtcatctggc
    24001 ggttgcgttt gagtctgacc cggaagcaga aatccgtcaa ctgaacagcc gtcgcgtcga
    24061 actggagcgg gcgttaagta atcatgaaaa tgataaccag cagcagcgta ttcagtttga
    24121 gcaggcgaaa gagggcgtta cggcgctgaa ccgcattctg ccgcgtctca acctgttggc
    24181 tgatgacagc ctggcggatc gcgtcgatga aatccgcgaa cgtctggatg aagcccagga
    24241 agccgcgcgt tttgttcagc agtttggcaa tcaactggcg aaactggaac cgattgtttc
    24301 ggtattgcag agcgacccgg aacagttcga acagttaaaa gaagattacg cgtactctca
    24361 gcagatgcag cgcgatgccc gtcagcaggc gtttgccctg acggaagtgg tgcagcgtcg
    24421 tgcgcacttt agctattctg actcggcaga aatgcttagc ggtaacagcg atctcaacga
    24481 aaaactgcgt gaacgtctgg aacaggcgga agcggagcgt acccgcgctc gcgaagcgtt
    24541 gcgcggtcac gcagcgcagt tgagtcagta caaccaggtg ctggcttcgc tgaaaagttc
    24601 ttacgacacc aaaaaagagc tactcaacga tctgcaacgt gaattgcagg atatcggcgt
    24661 gcgtgctgat agcggggcag aagagcgggc gcgtattcgc cgtgacgagc tgcatgcgca
    24721 actgagcaat aaccgttcac gccgcaatca actggaaaaa gcgcttacct tctgcgaagc
    24781 ggagatggac aacctgaccc gcaaactgcg caagctggag cgggattact ttgagatgcg
    24841 cgagcaggta gtgaccgcca aagcgggctg gtgtgcggtg atgcgcatgg tgaaagataa
    24901 cggcgttgag cgccgcttac accgtcgtga gctggcttat ctctccgctg atgatttgcg
    24961 ttccatgtcg gataaggcgt taggtgcgct gcgtctggcg gtggcggata acgaacatct
    25021 gcgcgacgtg ctgcgcatgt cggaagatcc gaaacgtccg gagcgtaaaa ttcagttctt
    25081 cgtggcggtt tatcagcatc tgcgtgaacg tattcgtcag gatattattc gtaccgatga
    25141 tccggtggaa gctatcgaac agatggagat tgaacttagc cgtctgaccg aagaattaac
    25201 ctcccgtgaa cagaaactgg cgatcagttc ccgcagcgtg gcgaacatca ttcgcaaaac
    25261 cattcagcgc gagcagaacc gtatccgtat gctcaaccag gggttgcaga acgtatcgtt
    25321 tggtcaggtg aacagcgtgc gtctcaacgt gaacgtgcgt gaaacgcacg ccatgctact
    25381 ggatgtgctc tctgaacagc acgagcagca tcaggatctg tttaacagca accgtttgac
    25441 cttctcggaa gcgctggcga aactgtatca acgtcttaac ccgcagattg atatggggca
    25501 gcgcacgccg cagaccattg gcgaagaact gctggattac cgcaactatc tggaaatgga
    25561 agttgaggtt aatcgtggtt ccgatggttg gttgcgcgca gagtctggtg cattgtcgac
    25621 cggtgaggcg attggtaccg gtatgtcgat tctggtgatg gtggtacaaa gctgggaaga
    25681 tgaatctcgc cgcctgcgcg gtaaagatat ctctccttgc cgcctgctgt tcctcgatga
    25741 agcagcgcga ctggatgctc gttctatcgc cacgctgttt gaattgtgtg aacgtttgca
    25801 aatgcagctc atcatcgcag cgccggaaaa tatcagcccg gagaaaggca ccacctataa
    25861 actggtgcgt aaagtcttcc agaataccga acacgttcac gtcgtcggcc tgcgaggatt
    25921 tgcgccgcaa ctccctgaaa cgcttccagg aagtgacgaa gcgccttctc aggcgagtta
    25981 aaattaaggc ggcagcaatg ccgccttttc tttttccgaa aactccgttt ctgcactaaa
    26041 aaagtggcac attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac
    26101 gtatcggcgt ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt
    26161 ttgtgagtat tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat
    26221 gataaaaaca gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca
    26281 atcagtttgt gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct
    26341 gaagtaatcc ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag
    26401 gcgcaagcca cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa
    26461 gcccgcacgc aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag
    26521 cttcaactgt tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt
    26581 aaagccttcc agcaacagct ggcagaggtg gcgattgccg gtttccagcc gcagtttaat
    26641 aaatgggtag agttactgac cgatcctggt gttaacggga tggcacgcga cgtagtgctc
    26701 tctgatgcga tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc
    26761 tggctatata gcagtaaacc ttatgcactt gcaacgccgc cgctctcggt gattaaccaa
    26821 tggcagctgg cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag
    26881 catccgcaat atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg
    26941 tggccccaac tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg
    27001 gcgttgcgcg aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta
    27061 cctggcgatg acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca
    27121 gcagaaacta agccgatgga caagcaaatg acgtctcgta gtaaacctgc gcctgccgtt
    27181 cgtgccgcct acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga
    27241 ttgggggcag atggtgctat tggcccggca acacgtgact ggttaaacgt aacgcccgcc
    27301 cagcgtgctg gcgtgttggc tctcaacatc cagcgattgc gcttgctgcc aacagagctt
    27361 tctaccggga tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat
    27421 caggtgctgg attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc
    27481 agtgccctta acaacgtagt ggtaaacccg ccgtggaacg tacctccaac tctggcacgc
    27541 aaagatattc tgccaaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg
    27601 gtgatgcgcg gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca
    27661 atcacggcct cgaatttacc gttccgcttc cagcaggctc caggcccacg gaactcgctg
    27721 gggcgctata aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac
    27781 cacaatctgt tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa
    27841 gcttccgatc tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct
    27901 gatgcgctga agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat
    27961 ctctactacc tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt
    28021 tacaattatg atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta
    28081 atcaggtaaa tgaagtagtt cggggaaatc agttgtcgta ataattagca tgattggggg
    28141 cgattctctg cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg
    28201 cggttaaggt gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac
    28261 ttgattatca tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt
    28321 gcactcggtg ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg
    28381 cgcattttga cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat
    28441 ggcagaggct atattcagga agaattggca aaacttaacc attttttccg cgattaccgc
    28501 gcgaacaaaa taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg
    28561 ttgttaggca cgcgcaaacc ggtgcaactc atttccggtt atcgttctat tgataccaac
    28621 aatgaactac gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag
    28681 gcgatggatt tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct
    28741 atgcgcgcag gtggtgtagg atattatcca cgtagtaact ttgtgcatat tgataccggg
    28801 ccagcacggc actggtagta attgcttaac gaaacagggg cagtatgaac tatcgtatta
    28861 ttccggtcac cgcattctcc cagaactgtt cattaatctg gtgtgaacaa acccgtctgg
    28921 ccgcactggt cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg
    28981 gcctgacact gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg
    29041 cggaactggc gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct
    29101 ggctgcaagg cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga
    29161 cgccagatcg ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg
    29221 tgttacattg ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc
    29281 tgctgatttc tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg
    29341 gcgatcataa tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg
    29401 tgacatttat tccgggtcac ggaccattat ccacacttgg ttatgaacgc ctgcataatc
    29461 ccttcctgca agacgaaatg cccgtctggt aaggcacata aaaaagcccg cttttaatgc
    29521 gggcctggat ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat
    29581 ccggcctaca aaatcgtgca aattcaaaat attgcagggg acgcgtagat actgataagc
    29641 gtagcgcatc aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc
    29701 attgttttta atgcttacag cactgccaca atcgcttcgc acagcggagc catgttatct
    29761 ggtgtcatcc cggccacatt tacgcgacca gaagcaaccg catatacgcc aaactcttcg
    29821 cgcagacgca gcacttgttc ttttgtcagg ccactgaagg agaacatgcc gttctgtttg
    29881 atgataaagc tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac gaacaactga
    29941 cgcatacgct gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaac
    30001 gcatcgttgc tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag
    30061 ttagcgcgaa tcgccgcttt catttggctg aatgcgcgat caacggtttc actgtcggca
    30121 gcaaccagag tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa
    30181 ctggcaacaa tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct
    30241 tccagaccac gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca
    30301 actgagagtt gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta
    30361 tggcagcagc catggaacag cactacgtcg ccagcctgag cttcattcag gctgttaatc
    30421 agtgcatcga agtcaagagt gtgattttcc gcatcataat aagcgtattc acgaacttcc
    30481 agacctgcag agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacact
    30541 cgcttaacgc tggtattttt tgccaggaaa tcggcagcta cgcgtagtgc gccagtgccc
    30601 cccggagtct gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac
    30661 agcagttcct gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg
    30721 gtggtttcat tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt
    30781 ttgcccgtct catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg
    30841 gcacgaaaca gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca
    30901 aacatgacga ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga
    30961 tttacaaaat tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt
    31021 gctctggaag gcataaaaaa aacaggacca aagtcctgtt ttttcggcat ttaacaaaga
    31081 ggtgtgctat tagaactggt aaacgatacc cacagcaacg gtgtcgtcag aacctacgcc
    31141 cagtttgttg tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt
    31201 gttgaagtag taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc
    31261 ttctacgtct ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa
    31321 ctggtattgc gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt
    31381 aaatttatta gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat
    31441 gttgttcgcg tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag
    31501 aggttgagct tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa
    31561 gccttcgtat tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt
    31621 gtcacgctcg tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa
    31681 gaagttggag ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct
    31741 gtatgcagta tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac
    31801 cacaccgtag ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa
    31861 tgccagacgc gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa
    31921 gttatattcc cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa
    31981 accaagacgg gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt
    32041 ggaaaaataa tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt
    32101 atagatttct gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag
    32161 aatattgcgc ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg
    32221 ccgtcaataa gttctgtcaa taaaaattta cggaactatt gatgagagtt tggtgtcttt
    32281 atgtgtctgc aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctacaa
    32341 agataaagat ttagtttcaa aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggaact
    32401 ttgtgaacac cgtcatattt ccatagaaac gtgatgatat ttacag
//
LOCUS       DAZFFI010000046        31889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFFI010000046 DAZFFI010000000
VERSION     DAZFFI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.046"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHJ12_002497"
     CDS             complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHJ12_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4990267.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHJ12_002498"
     CDS             complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHJ12_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4990268.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1749..2618
                     /gene="pldA"
                     /locus_tag="ACHJ12_002499"
     CDS             1749..2618
                     /gene="pldA"
                     /locus_tag="ACHJ12_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4990269.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2751..4580
                     /gene="recQ"
                     /locus_tag="ACHJ12_002500"
     CDS             2751..4580
                     /gene="recQ"
                     /locus_tag="ACHJ12_002500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4990270.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4644..5264
                     /gene="rhtC"
                     /locus_tag="ACHJ12_002501"
     CDS             4644..5264
                     /gene="rhtC"
                     /locus_tag="ACHJ12_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4990271.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5326..5946)
                     /gene="rhtB"
                     /locus_tag="ACHJ12_002502"
     CDS             complement(5326..5946)
                     /gene="rhtB"
                     /locus_tag="ACHJ12_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4990272.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6057..7079
                     /gene="pldB"
                     /locus_tag="ACHJ12_002503"
     CDS             6057..7079
                     /gene="pldB"
                     /locus_tag="ACHJ12_002503"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4990273.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7087..7887
                     /gene="yigL"
                     /locus_tag="ACHJ12_002504"
     CDS             7087..7887
                     /gene="yigL"
                     /locus_tag="ACHJ12_002504"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4990274.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7963..8862
                     /gene="bioP"
                     /locus_tag="ACHJ12_002505"
     CDS             7963..8862
                     /gene="bioP"
                     /locus_tag="ACHJ12_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4990275.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8750..9703)
                     /gene="metR"
                     /locus_tag="ACHJ12_002506"
     CDS             complement(8750..9703)
                     /gene="metR"
                     /locus_tag="ACHJ12_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4990276.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9821..12082
                     /gene="metE"
                     /locus_tag="ACHJ12_002507"
     CDS             9821..12082
                     /gene="metE"
                     /locus_tag="ACHJ12_002507"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4990277.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12121..12936)
                     /gene="ysgA"
                     /locus_tag="ACHJ12_002508"
     CDS             complement(12121..12936)
                     /gene="ysgA"
                     /locus_tag="ACHJ12_002508"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4990278.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13198..13959
                     /gene="udp"
                     /locus_tag="ACHJ12_002509"
     CDS             13198..13959
                     /gene="udp"
                     /locus_tag="ACHJ12_002509"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4990279.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14100..15527
                     /gene="rmuC"
                     /locus_tag="ACHJ12_002510"
     CDS             14100..15527
                     /gene="rmuC"
                     /locus_tag="ACHJ12_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4990280.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15622..16377
                     /gene="ubiE"
                     /locus_tag="ACHJ12_002511"
     CDS             15622..16377
                     /gene="ubiE"
                     /locus_tag="ACHJ12_002511"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4990281.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16391..16996
                     /gene="ubiJ"
                     /locus_tag="ACHJ12_002512"
     CDS             16391..16996
                     /gene="ubiJ"
                     /locus_tag="ACHJ12_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4990282.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16993..18633
                     /gene="ubiB"
                     /locus_tag="ACHJ12_002513"
     CDS             16993..18633
                     /gene="ubiB"
                     /locus_tag="ACHJ12_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4990283.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18712..18981
                     /gene="tatA"
                     /locus_tag="ACHJ12_002514"
     CDS             18712..18981
                     /gene="tatA"
                     /locus_tag="ACHJ12_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4990284.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18985..19500
                     /gene="tatB"
                     /locus_tag="ACHJ12_002515"
     CDS             18985..19500
                     /gene="tatB"
                     /locus_tag="ACHJ12_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4990285.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19503..20279
                     /gene="tatC"
                     /locus_tag="ACHJ12_002516"
     CDS             19503..20279
                     /gene="tatC"
                     /locus_tag="ACHJ12_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4990286.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20321..21103
                     /gene="tatD"
                     /locus_tag="ACHJ12_002517"
     CDS             20321..21103
                     /gene="tatD"
                     /locus_tag="ACHJ12_002517"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4990287.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21100..21588)
                     /gene="rfaH"
                     /locus_tag="ACHJ12_002518"
     CDS             complement(21100..21588)
                     /gene="rfaH"
                     /locus_tag="ACHJ12_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4990288.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21755..23248
                     /gene="ubiD"
                     /locus_tag="ACHJ12_002519"
     CDS             21755..23248
                     /gene="ubiD"
                     /locus_tag="ACHJ12_002519"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4990289.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23294..23995
                     /gene="fre"
                     /locus_tag="ACHJ12_002520"
     CDS             23294..23995
                     /gene="fre"
                     /locus_tag="ACHJ12_002520"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4990290.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24187..25350)
                     /gene="fadA"
                     /locus_tag="ACHJ12_002521"
     CDS             complement(24187..25350)
                     /gene="fadA"
                     /locus_tag="ACHJ12_002521"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4990291.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25360..27549)
                     /gene="fadB"
                     /locus_tag="ACHJ12_002522"
     CDS             complement(25360..27549)
                     /gene="fadB"
                     /locus_tag="ACHJ12_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4990292.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27739..29070
                     /gene="pepQ"
                     /locus_tag="ACHJ12_002523"
     CDS             27739..29070
                     /gene="pepQ"
                     /locus_tag="ACHJ12_002523"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4990293.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29070..29684
                     /gene="yigZ"
                     /locus_tag="ACHJ12_002524"
     CDS             29070..29684
                     /gene="yigZ"
                     /locus_tag="ACHJ12_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4990294.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29723..31174
                     /gene="trkH"
                     /locus_tag="ACHJ12_002525"
     CDS             29723..31174
                     /gene="trkH"
                     /locus_tag="ACHJ12_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4990295.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31186..31731
                     /gene="hemG"
                     /locus_tag="ACHJ12_002526"
     CDS             31186..31731
                     /gene="hemG"
                     /locus_tag="ACHJ12_002526"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4990296.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7388 a         8133 c         8732 g         7636 t
ORIGIN      
        1 ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg
       61 gagttttggg ggtacttcct gtaacaaata ttgttctttt tcttatctta atttttttca
      121 aaaaaagata aataagtcag tgggtaggct cattaaacgg cctacccatg ttaattctta
      181 actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat
      241 aaaggcgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag
      301 caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaggcg
      361 cgtggcagcg gcagtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag
      421 cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc
      481 aaacaggtaa atcgccgcca cgggcagcag ccacatggtt tcaattaaca tgccggtttg
      541 tgcttctacc gcaatcttct tgcgcaccag gccgtagaag gcaaaactaa atgccagccc
      601 cagcgcgata ataggtagcg aaccaaaagt ccacagctgg accaacacgc cacatatcgc
      661 cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag
      721 cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt
      781 gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag
      841 cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat
      901 gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc
      961 ggcgggcacg tagtaaatca acttgaagta cgctggcgca ataccccaaa taaaataagc
     1021 ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt
     1081 ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt
     1141 ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt
     1201 attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag
     1261 ataatcaacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc
     1321 actgatggtt tcgtggcggg ttaaggtact tcccacgcac accagaccgg cggcgacatc
     1381 cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc ccaccatcat
     1441 tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gttccatccc
     1501 caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg
     1561 ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga
     1621 ttttatgctt ctttgttgtt ggtttctact ttaggaaggg ataactaacg gcaatggagt
     1681 taagtatgta aaatagcccg cagaaaaaca ttcaccttat caataattcg ttacggagaa
     1741 cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag
     1801 tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta
     1861 ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca
     1921 actacctcat ctacacacaa accagcgatc tgaataaaga agcgattgcc agttacgact
     1981 gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccactgt
     2041 ggcgtgggat tttaggcccg aactcggtgt tgggtgcatc ttatacgcaa aaatcctggt
     2101 ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat
     2161 tgttcctcgg ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc gatgtggaga
     2221 tggggtataa ccacgactct aacgggcgtt cagacccgac ctcccgcagc tggaaccgcc
     2281 tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg
     2341 tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac taccagctta
     2401 aaatcggcta tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac aactggaaca
     2461 ccggctacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt
     2521 atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc
     2581 gtgtcggtgt gggagttatg ctaaacgatt tgttttgatg aacggttgag ttgttggcaa
     2641 atctggaatc cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc
     2701 gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg
     2761 cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc
     2821 aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc
     2881 tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc
     2941 taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc
     3001 aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgaacagc
     3061 aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg
     3121 aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag
     3181 ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg
     3241 cgctcggtca gttgcgccag cggttcccga cgctgccgtt tatggcgctg accgccacag
     3301 ccgacgacac cacgcgccag gatatcgtgc gcctgctggg gctgaacgat ccgctgattc
     3361 aaatcaacag ttttgaccgt ccgaatattc gctacatgct gatggagaag ttcaaaccgc
     3421 tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca
     3481 acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg
     3541 cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc
     3601 gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa
     3661 acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa
     3721 ccggtcgcgc cgggcgtgat ggcctgcccg cggaagcgat gctgttttac gatccggccg
     3781 atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatatcg
     3841 aacgtcataa actcaatgcg atgggcgctt ttgccgaagc gcaaacttgc cgtcgtctgg
     3901 tactgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc
     3961 tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg
     4021 gtcgtgtgaa tcagcgtttt ggtatgggct atgtggtgga agtgattcgc ggcgctaata
     4081 accagcgtat ccgcgactat ggtcatgaca aactaaaagt ctacggtatg ggccgtgata
     4141 aaagtcatga acattgggtg agcgtgatcc gccagttgat tcacctcggc ctggtgacgc
     4201 aaaatattgc ccagcattct gccctccaac tgacagaggc cgcgcgtccg gtgctgcgcg
     4261 gcgaatcctc tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc
     4321 agaaatcgtt cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta
     4381 aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga
     4441 ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg
     4501 ggatgcgcaa gctggaacgc tttggcaaac cgtttatggc gctgattcgt gcgcatgttg
     4561 atggcgatga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag actccgtaaa
     4621 cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca
     4681 ttgtggcgct tatgagtccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc
     4741 gttcccgtaa agaagcgatg atgggcgtgc tgggtattac ctgcggcgtt atggtttggg
     4801 cagggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc
     4861 tgattatggt tggcggtggc ctgtatctct gctggatggg ttatcagatg ctgcgcggcg
     4921 cgctgaaaaa agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc
     4981 gcagtttcct gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctattttg
     5041 gttcggtatt ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt
     5101 ttgcgctgat cattgtcgaa acactggcgt ggtttaccgt cgttgccagc ctgtttgccc
     5161 tgccgcaaat gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg
     5221 cgttatttgc cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc
     5281 agagtaagtc ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc
     5341 cgatgctaac agtgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt
     5401 catctgcttt ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat
     5461 catcacaata atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg
     5521 cggctgttgc ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg
     5581 attggtgaga ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc
     5641 cagcgattta aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa
     5701 gtaagccgcg cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt
     5761 ccccaacccc acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat
     5821 agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc
     5881 agagcctggc gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa
     5941 ggtcatgatg aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt
     6001 tgtgtcaggc acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt
     6061 ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac
     6121 cgctgactga tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc
     6181 cggtgcgctt tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg
     6241 ggcgtattga gagctacgta aaatatgcgg aactggccta tgacctgttc catttggggt
     6301 ttgatgtctt aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc
     6361 cgcatctcgg gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc
     6421 agcaggaggt tcagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg
     6481 gtgcgatctc cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa
     6541 ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca
     6601 actgggccga agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc
     6661 gcgcgttgcc gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact
     6721 tacgcttcta tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac
     6781 gcgaaagtat tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa
     6841 cgcttctctt acaagctgaa gaggaacgcg tggtggataa ccgcatgcat gaccgttttt
     6901 gtgaactccg caccgccgcg ggccatcctg tcgaaggagg acggccgttg gtaattaaag
     6961 gtgcttacca tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca
     7021 tcgttgattt tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa
     7081 tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat
     7141 acgttatccc cttacgccaa agaaactctg aagctgctca ccgcgcgcgg catcaacttt
     7201 gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatt
     7261 aagtcttaca tgattacctc caatggtgcg cgcgttcacg atctggatgg taatctgatt
     7321 tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat
     7381 ccggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg
     7441 gaagagatgc gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta
     7501 ctggagccgg aaggcgtcag caaagtgttt ttcacctgcg attcccatga acaactgctg
     7561 ccgctggagc aggcgattaa cgctcgctgg ggcgatcgcg tcaacgtcag tttctctacc
     7621 ttaacctgtc tggaagtgat ggcgggcggc gtgtcgaaag gccatgcgct ggaagcagtg
     7681 gcgaagaaac tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgac
     7741 gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg
     7801 aaagaccttc atcccgagct ggaagtgatt ggtactaacg ccgaagacgc ggtgccgcat
     7861 tatctgcgta aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata
     7921 cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc
     7981 accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc
     8041 gatagctatt ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt
     8101 ctgcgtaccc gtggcaatag cctgaaaaca gtcggcctgt atatgctggt gggcgcgatg
     8161 cagctcggcg tgatgtatat gctgagtttc cgcgcttatc tctacctgac ggtttccgag
     8221 ctgctgctgt tcaccgtgct gacgccgctc tacatcacgc tgatttatga catcatgagt
     8281 aagcgccgtc tgcgctgggg ctatgctttt agcgccttgc tggcggtgat tggtgccggg
     8341 attattcgct atgatcaggt caccgaccat ttctggactg gcttgttgct ggtgcaactc
     8401 tccaatatca cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg
     8461 atgccgcagc ataacgcctt tgcgtggttc tatcttggcg cgttcctggt ggcggtaatt
     8521 gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgctgca atggggcatt
     8581 ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgcg
     8641 acgcaggtgg acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg
     8701 ctggtaaacc tggctatctg gcaccaacag ccgcactggc caacgttcat tacaggcgcg
     8761 ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa
     8821 acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct
     8881 gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg
     8941 ttttggtcac caccagaccc tggcgctcaa aactctctac tacccaatgc ggtagcgcgg
     9001 caatacccat ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca
     9061 gtgacgggct gacgcctgcc ggctgaagaa aatgccgcca gacatccagt cgactacgct
     9121 gcaccggata aatcaacagc gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg
     9181 ccgccagtgg atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat
     9241 aatgcaggcc actgcgcggc aggatatcgg aggtcatcac cagatccagc tcgccctgtt
     9301 gcaacgcggg ctgcgggtca aacgtcacgc ccgatttaaa atccatctct acctgcggcc
     9361 agttcttatg gaaattttct aacgcgggcg tcagccactg aatacagcta tggcactcaa
     9421 tggcaatgcg cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc tggctaattt
     9481 gcggcagcac ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg
     9541 gctggctctt acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt
     9601 gagacagggc ggattgcgtc tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt
     9661 tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg
     9721 gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat
     9781 ctggacggct aaatactaaa aattccataa ggggcatata atgacaattc ttaatcacac
     9841 cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg
     9901 ggcggggaac tccacgcgtg aagaactgct gacagtaggg cgtgaactgc gtgctcgtca
     9961 ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta
    10021 cgatcatgtt ctgaccacca gtctgctgct gggtaacgtt ccgccacgtc atcagaacaa
    10081 agacggttcg gtagatatcg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg
    10141 cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt
    10201 gccggagttc gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggaggaagt
    10261 ggacgaggcg ctggcgctgg gccacaacgt taagcctgtg ctgctggggc cggttactta
    10321 cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat
    10381 tctgccggtt tatcagcaaa tgctggcaga actggcgaaa cgcggcatcg agtgggtaca
    10441 gattgatgaa cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc
    10501 cgcttacgac gcgctccagg gccaggtgaa actgctgctg accacttatt ttgaaggcgt
    10561 aacgccaaac ctcgacacga ttactgcgct gcctgttcag gggctgcatg tggaccttgt
    10621 acatggtaaa gatgacgttg ttgaactgca caagcgcctg ccttctgact ggttgctgtc
    10681 tgcgggtctg atcaatggtc gtaacgtctg gcgtgccgat cttagcgaga aatatgcgca
    10741 aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca
    10801 cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc
    10861 cttcgcccta caaaaatgcc atgaactggc actgctgcgc gatgcgctga acagtggtga
    10921 cacggcagct ctggcagagt ggagcgcccc gattcaggcg cgtcgtcact ctactcgtgt
    10981 acataatccg gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc
    11041 gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt tttaaactgc cagcgtggcc
    11101 gaccaccacg attggttcct tcccgcaaac cacggaaatt cgtaccctgc gtctggattt
    11161 caaaaagggc aatctcgacg ccaacaacta ccgcacgggc attgcggaac atatcaagca
    11221 ggccattgtt gagcaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg
    11281 taatgacatg gtggaatact ttggcgagca cctcgacgga tttgtcttta cgcaaaacgg
    11341 ttgggtacag agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgtgag
    11401 ccgaccggca ccgattaccg tggagtgggc gaaatatgcg cagtcgctga ccgacaaacc
    11461 ggtgaaaggg atgctgactg gtccggtgac gattctctgc tggtcgttcc cgcgtgaaga
    11521 tgtgagccgt gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg aagtggcgga
    11581 cctggaagcc gctggaattg gcatcatcca gattgatgaa ccggcgctgc gcgaaggttt
    11641 accgctgcgt cgtagcgact gggacgccta tctccagtgg ggcgtagagg ccttccgtat
    11701 caacgccgcc gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt
    11761 caacgacatc atggattcca ttgcggcgct ggatgcggat gtgattacca tcgaaacttc
    11821 gcgttctgac atggagttgc tggagtcgtt cgaagagttt gattatccca atgaaatcgg
    11881 tccaggcgtc tatgatattc actcgccaaa cgtaccgagc gtggaatgga ttgaagccct
    11941 gctgaagaaa gcggcaaaac gcattccggc agagcgcctg tgggttaacc cggactgtgg
    12001 cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc
    12061 gcagaacttg cgtcgggggt aaatccaaac cgggtggtaa taccacccgg tcttttctca
    12121 ttacagcgac ttcttcccac catactgctt aaaccattcc agcatacgct gccagccatc
    12181 tttcgcagat tcggcatgat agctcgggcg ataatcagcg ttgaatgcat gcccggcatc
    12241 cgggtacacg ataatctctg ctttcgcatt agcagcccgc agcgcctggc gcatggtttc
    12301 aacgctctcc tgcggaatgc tgttatcctg accaccatat aagccgagaa ccggcgcgtt
    12361 aagatcggtt gcgatatcaa caggttgttt cggtgaattc agcgacttgt cgcccgtcag
    12421 tttgccgtac cacgccactg cggcttttag ctgtggatta tgcgcggcat acagccaggt
    12481 gatacgtcca ccccagcaga atccggtgat cattaaacga tgaacatcgc cgccgttgcg
    12541 ggaagcccaa ctggcgacat gatcgagatc ggccagcacc tgcgagtcag gcacttttgc
    12601 taccagaccg ctaagcaacg tggggatatc ggcaaaatca ttcggatcgc cttcgcggaa
    12661 gtaaagttca ggtgcgatag ccagataccc ctccagcgcc agacggcgac aaatgtcgcg
    12721 gatatgttca tgcacgccaa aaatttcctg cactacaatg accactggca gttggccatc
    12781 gctttgcttt ggtctggcat ggtaagcagg catgttatcc ccttgtgaag ggatagaggt
    12841 gaagcccgcc acaatcgcgt cgtccggggt ctgaacgatg gtcgaagcga gaggcgatgc
    12901 agcaggtgta aatccagatt gttgtgttgt tgccatggta ttctccgtac ccttataaaa
    12961 atgttgcgca atgttaacta tagtcagcat gcaacaaatc acattgcctg aatcggctca
    13021 tcttttatgc agtcctgcag aatgaagagt gatttatgtg atttgaatca cttttggtgg
    13081 gtaaatttat gcaacgcatt tgcaccatgg tgatgagtat cacgaaaaaa tgttaaaccc
    13141 ttcggtaaag tgtctttttg cttcttctga ctaaaccgat tcacagagga gttgtatatg
    13201 tccaagtctg atgtttttca tctcggcctc actaaaaacg atttacaagg ggctacgctt
    13261 gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg ccgcgctgat ggataagccg
    13321 gttaagctgg catctcaccg cgaattcact acctggcgtg cagagctgga tggtaaacct
    13381 gttatcgtct gctctaccgg tatcggcggc ccgtctacct ctattgctgt tgaagagctg
    13441 gcacagctgg gcattcgcac cttcctgcgt atcggtacaa cgggcgctat tcagccgcat
    13501 attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc gtctggatgg cgcgagcctg
    13561 cacttcgcac cgctggaatt cccggctgtc gctgatttcg aatgtacgac tgcgctggtt
    13621 gaagctgcga aatccattgg cgcgacaact cacgttggcg tgacagcttc ttctgatacc
    13681 ttctacccag gtcaggaacg ttacgatact tactctggtc gcgtagttcg tcactttaaa
    13741 ggttctatgg aagagtggca ggcgatgggc gtaatgaact atgaaatgga atctgcaacc
    13801 ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta tggtagcggg tgttatcgtt
    13861 aaccgcaccc agcaagagat cccgaatgct gagacgatga aacaaaccga aagccatgcg
    13921 gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat tctcttctcc tgtctgaagg
    13981 ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg caggaaatgc ctttccaact
    14041 ggacgtttgt acagcacaat tctattttgt gcgggtaagt tgttgcgtca ggaggcgttg
    14101 tggatttctc aatcatggtt tacgcagtta ttgcgttggt gggtgtggca attggctggc
    14161 tgtttgccag ttatcaacat gcgcagcaaa aagccgagca attagctgaa cgtgaagaga
    14221 tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca aagcgagcac tggcgtgcag
    14281 agtgcgagtt actcaataac gaagtgcgca gcctgcaaag tattaacacc tctctggagg
    14341 ccgatctgcg tgaagtaacc acgcggatgg aagccgcaca gcaacatgct gacgataaaa
    14401 ttcgccagat gattaacagc gagcagcgcc tcagtgagca gtttgaaaac ctcgccaacc
    14461 gtatttttga gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc
    14521 tgttgtcgcc gctacgtgaa caactggacg gtttccgccg tcaggttcag gacagcttcg
    14581 gtaaagaagc acaagaacgc cataccctga cccacgaaat tcgcaatctc cagcaactca
    14641 acgcgcaaat ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc gacaataaaa
    14701 cccagggcaa ctggggcgag gtagtattga cgcgggtgct ggaggcttcc ggtctgcgtg
    14761 aagggtatga atatgaaacc caggtcagca tcgaaaatga cgcccgctcg cggatgcagc
    14821 cggatgtcat cgtgcggctg ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc
    14881 tggtcgccta tgaacgctat tttaacgccg aagacgacta cacccgcgaa agcgcgctac
    14941 aggaacatat cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa gattatcaac
    15001 agctgccggg gctgcgaact ctggattacg tgctgatgtt tattcccgtt gaacccgctt
    15061 ttttactggc gcttgaccgc cagccggagc tgatcaccga agcgttgaaa aacaatatca
    15121 tgctggttag cccgactacg ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt
    15181 atgagcatca aagccgcaac gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca
    15241 agatgcgttt gttcatcgat gacatgtccg cgattggtca aagtctcgac aaagcgcagg
    15301 ataactaccg ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg
    15361 cagaagcgtt tcgcggttta ggagtagaaa ttaaacgcga gattaatccg gatttggctg
    15421 aacaggcggt gagccaggat gaagagtatc gacttcggtc ggttccggag cagccgaatg
    15481 atgaagctta tcaacgcgat gatgaatata atcagcagtc gcgctagccc attaggggta
    15541 gttaagccgg gtagaaatct agggcatcga cgcccaatct gttacacttc tggaacaatt
    15601 ttttgatgag caggcattga gatggtggat aagtcacaag aaacgacgca ctttggtttt
    15661 cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc acgttttcca ttccgtggca
    15721 tcaaaatacg atgtcatgaa tgatttgatg tcatttggta ttcatcgttt gtggaagcga
    15781 ttcacgattg attgcagcgg cgtacgccgt gggcagaccg tgctggatct ggctggtggc
    15841 accggcgacc tgacagcgaa attctcccgc ctggtcggag aaactggcaa agtggtcctt
    15901 gctgatatca atgaatccat gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg
    15961 attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc ggataacacc
    16021 tttgattgca tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga taaagcactg
    16081 cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga gttctcgaag
    16141 ccaattatcg agccgctgag caaagcctat gatgcatact ccttccatgt gctgccgcgt
    16201 attggctcac tggtcgcgaa cgacgccgac agctaccgtt atctggcaga atccatccgt
    16261 atgcatcccg atcaggatac cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc
    16321 gactactaca atctgacggc aggggttgtg gcgctgcatc gtggttataa gttctgacag
    16381 gagaccggaa atgcctttta aacctttagt gacggcagga attgaaagtc tgctcaacac
    16441 cttcctgtat cgctcacccg cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt
    16501 gcgcgtggag gtaaaaggct tttcgacgtc attgattctg gtgttcagcg aacgccaggt
    16561 tgatgtactg ggcgaatggg caggcgatgc tgactgcacc gttatcgcct acgccagtgt
    16621 gttgccgaaa cttcgcgatc gccagcagct taccgcactg attcgcagtg gtgagctgga
    16681 agtgcagggc gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt
    16741 cgaccctgcg gaactgctgg ccccttatac cggtgatatc gccgctgaag gaatcagcaa
    16801 agccatgcgc ggaggcgcaa agttcctgca tcacggcatt aagcgccagc aacgttatgt
    16861 ggcggaagcc attactgaag agtggcgtat ggcacccggt ccgcttgaag tggcctggtt
    16921 tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa
    16981 actggaggct aaatgacgcc aggtgaagta cggcgcctat atttcatcat tcgcactttt
    17041 ttaagctacg gacttgatga actgatcccc aaaatgcgta tcaccctgcc gctacggcta
    17101 tggcgatact cattattctg gatgccaaat cggcataaag acaaactttt aggtgagcga
    17161 ctacgactgg ccctgcaaga actggggccg gtttggatca agttcgggca aatgttatca
    17221 acccgccgcg atctttttcc accgcatatt gccgatcagc tggcgttatt gcaggacaaa
    17281 gttgctccgt ttgatggcaa gctggcgaag cagcagattg aagctgcaat gggcggcttg
    17341 ccggtagaag cgtggtttga cgattttgaa atcaagccgc tggcttctgc ttctatcgcc
    17401 caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc
    17461 ccggatattt tgccggtcat taaagcggat ctgaaactta tctaccgtct ggctcgctgg
    17521 gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac
    17581 gaaaagacat tgattgatga actgaatttg ctgcgggaat ctgccaacgc cattcagctt
    17641 cggcgcaatt ttgaagacag cccgatgctc tacatcccgg aagtttaccc tgactattgt
    17701 agtgaaggga tgatggtgat ggagcgcatt tacggcattc cggtgtctga cgttgcggcg
    17761 ctggagaaaa acggcactaa catgaaattg ctggcggaac gcggcgtgca ggtgttcttc
    17821 actcaggtct ttcgcgacag ctttttccat gccgatatgc accctggcaa catcttcgta
    17881 agctatgaac acccggaaaa cccgaaatat atcggcattg attgcgggat tgttggctcg
    17941 ctaaacaaag aagataaacg ctatctggca gaaaacttta tcgccttctt taatcgcgac
    18001 tatcgcaaag tggcagagct acacgtcgat tcaggctggg tgccaccaga taccaacgtt
    18061 gaagagttcg aatttgccat tcgtacggtc tgtgaaccta tctttgagaa accgctggcc
    18121 gaaatttcgt ttggacatgt actgttaaat ctgtttaata cggcgcgtcg cttcaatatg
    18181 gaagtgcagc cgcaactggt gttactccag aaaaccctgc tctacgtcga aggggtagga
    18241 cgccagcttt atccgcagct cgatttatgg aaaacagcga agcctttcct ggagtcgtgg
    18301 attaaagatc aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc
    18361 tgggtcgaaa aaatgccaga actgcctgaa ctggtttacg acagtttgcg ccagggcaag
    18421 tatttacagc acagtgttga taagattgcc cgcgagcttc agtcaaatca tgtacgtcag
    18481 ggacaatcgc gttattttct cggaattggc gctacgttag tattaagtgg cacattcttg
    18541 ttggtcagcc gacctgaatg ggggctgatg cccggctggt taatggcagg tggtctgatc
    18601 gcctggtttg tcggttggcg caaaacacgc tgattttttc atcgctcaag gcgggccgtg
    18661 taacgtataa tgcggccttg tttaatcatc atctaccaca gaggaacatg tatgggtggt
    18721 atcagtattt ggcagttatt gattattgcc gtcatcgttg tactgctttt tggcaccaaa
    18781 aagctcggct ccatcggttc cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg
    18841 agcgatgatg aaccaaagca ggataaaacc agtcaggatg ctgattttac tgcgaaaact
    18901 atcgccgata agcaggcgga tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc
    18961 cacgataaag agcaggtgta atccgtgttt gatatcggtt ttagcgaact gctattggtg
    19021 ttcatcatcg gcctcgtcgt tctggggccg caacgactgc ctgtggcggt aaaaacggta
    19081 gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga actgacccag
    19141 gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact
    19201 aacctgacac ccgaactgaa agcgtcgatg gatgaactac gccaggccgc ggagtcgatg
    19261 aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca caccatccat
    19321 aacccggtgg tgaaagataa tgaagctgcg catgagggcg taacgcctgc cgctgcacaa
    19381 acgcaggcca gttcgccgga acagaagcca gaaaccacgc cagagccggt ggtaaaacct
    19441 gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa
    19501 acatgtctgt agaagatact caaccgctta tcacgcatct gattgagctg cgtaagcgtc
    19561 tgctgaactg cattatcgcg gtgatcgtga tattcctgtg tctggtctat ttcgccaatg
    19621 acatctatca cctggtatcc gcgccattga tcaagcagtt gccgcaaggt tcaacgatga
    19681 tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc
    19741 tgattctgtc agcgccggtg attctctatc aggtgtgggc atttatcgcc ccagcgctgt
    19801 ataagcatga acgtcgcctg gtggtgccgc tgctggtttc cagctctctg ctgttttata
    19861 tcggcatggc attcgcctac tttgtggtct ttccgctggc atttggcttc cttgccaata
    19921 ccgcgccgga aggggtacag gtatccaccg acatcgccag ctatttaagc ttcgttatgg
    19981 cgttgtttat ggcgtttggt gtctcctttg aagtgccggt ggcaattgtg ctgctgtgct
    20041 ggatggggat tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg
    20101 cattcgttgt cgggatgctg ctgacaccgc cggatgtctt ctcgcaaacg ctgttggcga
    20161 tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc acgcttttac gttggtaaag
    20221 ggcgaaatcg ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact gaagaataaa
    20281 ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg atgtttgata tcggcgttaa
    20341 tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc
    20401 gggagttaat gggctactca tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa
    20461 gctggcgcgt cagtattcgt cctgttggtc aacggcgggc gtacatcctc acgacagcag
    20521 ccagtggcaa gctgcgactg aagaagcgat tattgagctg gccgcgcagc cagaagtggt
    20581 ggcgattggt gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg aagagcagga
    20641 acgcgctttt gttgcccagc tacgcattgc cgcagattta aacatgccgg tatttatgca
    20701 ctgtcgcgat gcccacgagc ggtttatgac attgctggag ccgtggctgg ataaactgcc
    20761 tggtgcggtt cttcattgct ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg
    20821 tggaatttat atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg
    20881 ggaattgttg ccgttgattc cggcggagaa attgctgatc gaaactgatg cgccgtatct
    20941 gctcccgcgc gatctcacgc caaagccatc atcccggcgc aacgagccag cccatctgcc
    21001 ccatattttg caacgtattg cgcactggcg tggagaagat gccgcatggc tggctgccac
    21061 cacggatgcc aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc ggaactcggt
    21121 gttcttcaca ctgtgcttaa tctctttatt aataagatta agcaatagca tggagcgagc
    21181 ctcaccatcg ggttcggtga aaatggcctg aaagccttcg aacgcgcctt cggtaataat
    21241 caccttatca cccggataag gggttgccgg atcgacaatg tctttcggtt tatataccga
    21301 tagctgatga atcaccgccg atgggactat cgctggcgac gcgccaaagc gcacgaagtg
    21361 gctgacaccg cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat caaattcgac
    21421 aaacaggtag ttgggaaaca atggctcact gactgcagta cgttttccac gcacgatttt
    21481 ttccagggtg atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc
    21541 acgttgaagt tgcccgcgct tgcagtacag taaataccag gattgcataa tgactcttat
    21601 ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct aagttaatta tattccccgg
    21661 tttgcgttat accgtcagag ttcacgctaa tttaacaaat ttacagcatc gcaaagatga
    21721 acgccgtata atgggcgtag attaagaggc tacaatggac gccatgaaat ataacgattt
    21781 acgcgacttc ttgacgctgc ttgaacagca gggtgagcta aaacgtatca cgctcccggt
    21841 ggatccgcat ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc
    21901 gctgttgttc gaaaacccta aaggctactc aatgccggtg ctgtgcaacc tgttcggtac
    21961 gccaaagcgc gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg
    22021 taaattattg gcgtttctga aagagccgga gccgccaaaa ggtttccgcg acctgtttga
    22081 taaactgccg cagtttaagc aagtattgaa catgccgaca aagcggctgc gtggtgcgcc
    22141 ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc ccattatgac
    22201 ctgctggccg gaagatgccg cgccgctgat tacctggggg ctgacagtga cgcgcggccc
    22261 gcataaagag cggcagaatc tgggcattta tcgccagcag ctgattggta aaaacaaact
    22321 gattatgcgc tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt ggtgtgcggc
    22381 gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt gccgatcccg ccacgattct
    22441 cggtgcagtc acccccgttc cggatacact ttcagagtat gcgtttgccg gattgctgcg
    22501 cggtaccaag accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc
    22561 ggagattgtg ctggaagggt atatcgaaca aggtgaaacg gcgccggaag ggccgtatgg
    22621 cgaccacacc ggttactata acgaagtcga tagtttcccg gtatttaccg tgacgcatat
    22681 tacccagcgt gaagatgcga tttaccactc cacctatact ggtcgtccgc cggatgaacc
    22741 cgcggtgctg ggtgtcgcac tgaacgaagt gtttgtgccg attctgcaaa aacagttccc
    22801 ggaaattgtc gatttttacc tgccgccgga aggctgttct tatcgtctgg cggtagtgac
    22861 gatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt
    22921 acgccagttt atgtacacta aatttgtgat cgtttgcgat gatgacgtca acgcacgcga
    22981 ctggaacgat gtgatttggg cgattaccac ccgtatggac ccggcgcggg atactgttct
    23041 ggtagaaaat acgcctattg attatctgga ttttgcctcg cctgtctccg ggctgggttc
    23101 aaaaatgggg ctggatgcca cgaataaatg gccgggggaa acccagcgtg aatggggacg
    23161 tcccatcaaa aaagatccag atgttgtcgc gcatattgac gccatctggg atgaactggc
    23221 tatttttaac aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg
    23281 acagagaaag cgcatgacaa ccttaagctg taaagtgacc tcggtagaag ctatcacgga
    23341 taccgtatat cgtgtccgca tcgtgccaga tgcggccttt tcttttcgcg ctggtcagta
    23401 tttgatggta gtgatggatg agcgcgacaa acgtccgttc tcaatggctt cgacgccgga
    23461 tgaaaaaggg tttatcgagc tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc
    23521 agtcatggac cgcatcctca aagatcatca aatcgtggtc gatattcccc acggagaagc
    23581 gtggctgcgc gatgatgaag agcgtccgat gattttgatt gcgggcggca ccgggttctc
    23641 ttatgcccgc tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat
    23701 ttactggggc gggcgtgaag agcagcatct gtatgatctc tgcgagcttg aggcgctttc
    23761 gttgaagcat cctggtctgc aagtggtgcc ggtggttgaa caaccggaag cgggctggcg
    23821 tgggcgtact ggcaccgtgt taacggcggt attgcaggat cacggtacgc tggcagagca
    23881 tgatatctat attgccggac gttttgagat ggcgaaaatt gcccgcgatc tgttttgcag
    23941 tgagcgtaat gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta tctgagatat
    24001 aaaaaaaccc gcccctgaca ggcgggaaga acggcaacta aaactttatt cagtggcatt
    24061 tagatctatg tcgcatccgg cattcggcac acgatgcctg atgcgacgct ggcgcgtctt
    24121 atcaggccta cattggtgcc ggatcggtag accggataag gcgttcacgc cgcatccggc
    24181 aagtggttaa acccgctcaa acaccgtcgc aataccctga cccagaccga tacacatcgt
    24241 cgccagacca aactggacgt ctttgcgctc catcagattt agcagcgtgg tgctgatacg
    24301 cgcaccggaa cagcccagcg gatgacccag cgcgatcgcg ccaccgttga ggttgatctt
    24361 ctcgtcaatc tgctccatta gccccagatc tttaatacat ggcaggatct gcgcggcaaa
    24421 ggcttcattc atttcaaaca cgccgatatc actgacagaa agccccgctt ttttcagcgc
    24481 cagcttcgag gccggaaccg ggccgtaacc cataatcgat gggtcacaac caacgaccgc
    24541 catcgaacgc acgcgggcgc gcggcttaag acctaattca tgggcgcggc tttcactcat
    24601 caccagcatg gcagctgcgc catcggaaag cgcagaagat gtacccgccg ttaccgtacc
    24661 gcttactgga tcaaacgccg gacgcagcgt ggctagggct tccacggtgg tttccgggcg
    24721 aatcacttcg tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac caccggtcgg
    24781 gataatttca tttttaaatg cacccgactg cgtggcggcc caggcgcggg cgtgtgaccg
    24841 cgcggcaaag gcatcctgca tttcacggct gataccgtgc atacgcgcca gcatttctgc
    24901 cgttaagccc atcatgcccg ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc
    24961 gacgccgtgg ctcattggca catggcccat atgctccacg ccgccgacaa gacatgcctg
    25021 cgcatcgcca gtcatgatca ttcgtgctgc gtcatgcaat gcctgcatgg atgaaccaca
    25081 caagcgattg acggtcaccg ccgggacaga gtgtggtact tctgccagca gcgccgcgtt
    25141 acgggcgata ttaaaaccct gctccagcgt ctgctgcaca caaccccagt aaatatcgtc
    25201 gagggccgcc gcttccagcg ccgggttacg cgccagcagg ctacgcatta aatgagcgga
    25261 gagatcttcc gcacgcacgt tacgaaaagc accgcccttc gaacggccca tcggggtgcg
    25321 aattgcatcg acaatgacaa cctgttccat tgtgactcct taagccgttt tcaggtcgcc
    25381 aaccggacgg gctggctcaa ccgggggata gtacggttcg ttatgacgtg ctttattacg
    25441 cagaccttcc ggcacttcgt acagcgggcc gaggtgctga tattgctgtg ccatatcgag
    25501 gtattttgcg ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga acggagggaa
    25561 gcccaggccg tagaccagcg ccatatccgc ttccgccgga gtggcgataa tgccttcctc
    25621 cagacagcgc accacttcgt tgaccatcgg gatcatcatg cgggcgataa tctcttcttc
    25681 gctgaaatcg cgcttcggct ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc
    25741 ttctttcttc ggcttacctt tgctgtcttc tttataacgc cagaaaccga ggccgttctt
    25801 ctgaccaaag cggttggcat caaacagcgc gtcgatggca tcgcggtaat ctttctgcat
    25861 ccgctgcggg aagcctgctg ccatgacagc ctgagcgtga tgcgcggtat caatgcccac
    25921 gacatccagc agatatgccg gacccatagg ccagccaaac tgtttttcca tcactttgtc
    25981 gatcttgcgg aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga aatacgggaa
    26041 cagcacgcgg ttaacaaaga agccggggca gtcgttaacc acaatcggcg tcttgcccat
    26101 cttgctcgcc caggcgacaa ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat
    26161 aatttctacc aacggcatcc ggtgtaccgg gttaaaaaag tgcattccgc agaagttttc
    26221 cgggcgttcc agcgcgttgg ccagttcgct gataggaatg gttgaagtgt tagacgccag
    26281 cacggtatcc gggcgtactt tttgttcggt ttctgccagt acggcttttt tcactttcgg
    26341 gttttcgaca accgcttcta ccacaacatc cacgcggtca aacccggcgt agtcgagcgt
    26401 tgggtggatt gtggagatca cgccagccag tttcagacca tcgatcttgc cgcgctcaag
    26461 ctgcttgttc agcagtttcg cggcttcggt catgccgagg gttaacgact tgtcgttgat
    26521 atctttcatg acaaccggca cgcctttcca cgcagactgg taagcgatgc cgccgcccat
    26581 aatgcctgca cccagcaccg cggcctgttt cggggtttca acgtctttgg tgagtttctt
    26641 cgctttgcct tttacatatt gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt
    26701 ggtatgcgcc agcgggacaa aacttttgtt ttccaggttt aaggcttctt cacgaccaaa
    26761 acgggccgca gcttcaatgg tttttactgc ggtgatgggg gccggataat gtttccccgc
    26821 tgtttgtgcg accatccctt tagcgatggt gaagctcatg gtggcttcaa tcttgctcag
    26881 ttttagtggt tccagcttcg gctgacgttt tgctttccag tcgaggtcgc cgttaatggc
    26941 ctggcgtaaa accgcctttg cgccttcaac cagtttttct gctttgacta cgccatccac
    27001 cagaccgatt ttcagcgcct gatccgcgcc gacatcttta ccggcggcaa tgatttccag
    27061 cgcactgtca gcgcccagca tacgtggcat acgtacagaa ccgccaaagc ccggcatgat
    27121 acccagtttg gtttccggca gaccgatgcg cagatccggc gtcgccagac gataatcggt
    27181 cgccagcacg cattcgcagc caccgcccag cgcatagccg ttgacggcag caatggtcgg
    27241 caccggcaga tcttccaggc gattaaacac gctattggca aattgcagcc actgacttaa
    27301 ctgttcttca ggaacgagga acagggacaa aaattcggtg atatcagcac cgacgataaa
    27361 ggctgctttg ttcgaacgca gcagcagccc ttttagatct gattgctgtt ccagcacgcc
    27421 gatggcctcg ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg aacctggggc
    27481 atcaaatacc agttcggcaa tgccatcttc cagccagtca aggtacaggg tgtcgccttt
    27541 gtaaagcatg tcagtctcct gaatccgcaa ggtgatctgg tcgtaccaga tgagtcgaag
    27601 tgtgtatttt gtgttaaaaa tatgcaaaca aaagattaaa gaaatgccga tctgatcacg
    27661 ctcggcagaa atcacgctct ggatgaacga tgtgctaaga tgcggagact taaggtcaaa
    27721 aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct ataaaaatca tatagctacc
    27781 ttacaagaac ggactcgcga tgcgctggcg cgcttcaagc tggatgcgtt acttattcac
    27841 tccggcgagc tgttcaatgt ttttctcgac gatcatccct atccgtttaa agtgaacccg
    27901 caattcaaag cgtgggtgcc ggtaactcag gtgccaaact gctggttgct ggtggatggc
    27961 gtgaacaagc cgaaactgtg gttttatctg ccggttgatt actggcacaa cgtcgaaccg
    28021 ctgccgacct ccttctggac tgaagatgtg gaagtgatcg cgctgccgaa agccgatggc
    28081 attggtagcc tgctgcctgc tgcgcgcggc aatatcggtt atatcggtcc ggtgccggag
    28141 cgtgcgctgc aactgggtat tgaggccagc aacatcaacc cgaaaggggt tatcgactac
    28201 ctgcattact atcgctcctt caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag
    28261 aaaatggcgg tcaacggtca tcgtgcggca gaagaagcgt tccgttctgg catgagcgag
    28321 ttcgatatca acattgccta tctgaccgcg accggtcatc gtgataccga cgtaccttac
    28381 agcaacattg tggcgcttaa cgaacacgct gcggtgctgc attacaccaa actggatcat
    28441 caggcgccgg aagagatgcg cagcttcctg ctggatgccg gggccgaata taacggctat
    28501 gcggctgacc tgactcgtac ctggtcggca aaaagcgaca acgactacgc acagctggtg
    28561 aaagacgtaa atgatgaaca actggcgctg atcgccacca tgaaagcagg cgttagctat
    28621 gtggattacc acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc
    28681 atcaccgata tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc gtttatgccg
    28741 cacggtatcg gtcatccgct gggcctgcag gtgcatgacg tagccggttt tatgcaggat
    28801 gatagcggta cacacctcgc ggcaccggca aaatatccgt acctgcgctg cacccgtatt
    28861 ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct acttcattga atcgctactg
    28921 gcaccgtggc gtgaagggca gttcagcaag cacttcaact ggcagaaaat tgaagcactg
    28981 aaaccgttcg gcggcattcg tatcgaagac aacgtggtga tccacgaaaa caacgtggaa
    29041 aacatgaccc gggatctgaa actggcgtga tggaaagctg gttaattcct gcggcaccgg
    29101 tcacggtcgt tgaagagatc aaaaagagcc gcttcataac actgttggcg cataccgatg
    29161 gcgttgaggc ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc gatgcccgcc
    29221 accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc tcaacagctg ggtttctctg
    29281 acgacgggga gccggcggga acggcaggta aaccgatgct cgcccagcta atgggcagcg
    29341 gcgtcgggga aattaccgct gtggtagtgc gctactacgg cggcatattg cttggcaccg
    29401 gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc
    29461 aacgcaagac gccattaacc gaatatactt tgcaatgtga atatcatcag ttaaccggca
    29521 ttgaagcgtt gctggggcag tgtgacggca aaattatcaa cagtgattat caggcattcg
    29581 ttctgctgcg ggtggcgctt ccggcggcga aagtggctga attttccgca aagctggcgg
    29641 attttagccg tggttcattg caattgttag cgattgaaga ataatcccca cttcgttttg
    29701 cagaactaag gaagcggcag agatgcattt tcgcgccatt acccgaatcg ttggactact
    29761 ggtcatctta ttttcaggga ccatgattat ccctgggctg gtagcactca tctaccggga
    29821 tggagcgggc cgcgctttta cccagacctt ttttgtcgcc ctcgccattg gctctatgct
    29881 gtggtggccg aaccgcaaag agaaaggcga acttaaatcc cgtgaggggt ttctgatagt
    29941 ggtgctgttc tggaccgtgc tgggtagcgt cggtgcgctc ccttttatct tctcggaaag
    30001 cccgaacctc acgattaccg atgcgttttt tgaatctttc tctggcctga ccaccaccgg
    30061 ggccactacg ctggtggggc tggattcgct ccctcacgcc atcctctttt atcgccagat
    30121 gctgcaatgg tttggcggga tggggatcat cgtgttagcg gttgcgatac tgcctatcct
    30181 cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc ggcccgctga aagataacaa
    30241 aatgcgcccg cgaattgcgg aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac
    30301 cgtcgcctgt gcgctggcgt tgtggtttgc cggaatggat gcctttgatg ccatcggcca
    30361 tagctttgcg actatcgcta ttggcggctt ctcgacacat gatgccagta tcggttattt
    30421 cgatagcccg actattaaca ctatcattgc tatcttcctg ctgatctccg gctgtaacta
    30481 cggtctgcac ttttcactgt taagtgggcg tagtctgaag gtttattggc gcgatccgga
    30541 atttcgcatg tttatcggcg tacagtttac gctggtggtt atttgtacac tcgtactgtg
    30601 gtttcataat gtctacagtt cggcgctgat gacaattaac caggcgtttt tccaggtggt
    30661 atcgatggcg acaaccgccg ggtttacgac tgacagcatt gcccgctggc cgctcttttt
    30721 gccggtactg cttttatgtt cagcttttat cggcggttgc gccgggtcaa cgggcggtgg
    30781 cctgaaagtg atccgcatcc tgctgctgtt taagcagggg aaccgtgagc tgaaacgact
    30841 ggtgcatccg aacgccgtct atagcattaa gctggggaat cgcgcactgc cggaacgtat
    30901 cctcgaagcc gtttggggat ttttctccgc ctatgcattg gtgtttattg tcagtatgct
    30961 ggcgattatc gccacgggcg tggatgactt ttctgccttt gcctcagttg ttgcgacatt
    31021 gaataacctg gggccagggc ttggcgtggt tgctgataac tttaccagta tgaacccggt
    31081 ggctaaatgg atcctgattg ccaacatgct gtttggtcgt ctcgaggtct ttacattgct
    31141 ggtgctcttt accccgactt tctggcgtga atgatggagt aatacgtgaa aacattaatt
    31201 cttttctcaa caagggacgg acaaacgcgc gagattgcct cctacctggc ttcggaactg
    31261 aaagaactgg ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg
    31321 gaaaattatg accgtgtggt cattggtgct tctattcgct atggtcacta ccactctgcg
    31381 ttccaggaat ttgtcaaaaa gcatgcgacg cgactgaatt cgatgccgag cgccttttat
    31441 tccgtaaacc tggtggcgcg caaaccggag aagcgtactc cacagaccaa cagctacgcg
    31501 cggaagtttc tgatgaactc gcaatggcgt cccgatcgct gtgccgtcat tgccggggcg
    31561 ctgcgttacc cacgttatcg ctggtacgac cgttttatga tcaagctgat tatgaagatg
    31621 tcaggcggtg aaacggatac gcgcaaagaa gttgtctata ccgattggga gcaggtggcg
    31681 aatttcgccc gagaaatcgc ccatttaacc gacaaaccga cgctgaaata agcataaaga
    31741 ataaaaaatg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc
    31801 cctataatgc gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt
    31861 tctcctgaga atctcaacag agaaaagta
//
LOCUS       DAZFFI010000047        31819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFFI010000047 DAZFFI010000000
VERSION     DAZFFI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.047"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..288)
                     /gene="stfP"
                     /locus_tag="ACHJ12_002527"
     CDS             complement(<1..288)
                     /gene="stfP"
                     /locus_tag="ACHJ12_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein StfP"
                     /protein_id="HFO4990297.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALEN"
     gene            complement(292..876)
                     /gene="ymfQ"
                     /locus_tag="ACHJ12_002528"
     CDS             complement(292..876)
                     /gene="ymfQ"
                     /locus_tag="ACHJ12_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFO4990298.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPTGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDANITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(867..1925)
                     /locus_tag="ACHJ12_002529"
     CDS             complement(867..1925)
                     /locus_tag="ACHJ12_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO4990299.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(1912..2337)
                     /locus_tag="ACHJ12_002530"
     CDS             complement(1912..2337)
                     /locus_tag="ACHJ12_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO4990300.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(2337..2885)
                     /locus_tag="ACHJ12_002531"
     CDS             complement(2337..2885)
                     /locus_tag="ACHJ12_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO4990301.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(2885..3964)
                     /locus_tag="ACHJ12_002532"
     CDS             complement(2885..3964)
                     /locus_tag="ACHJ12_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO4990302.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAKPTQFNGRSLVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFISRYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(3961..5289)
                     /locus_tag="ACHJ12_002533"
     CDS             complement(3961..5289)
                     /locus_tag="ACHJ12_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO4990303.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(5350..7185)
                     /locus_tag="ACHJ12_002534"
     CDS             complement(5350..7185)
                     /locus_tag="ACHJ12_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4990304.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTVSE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTYVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGRELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPVVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(7178..7369)
                     /locus_tag="ACHJ12_002535"
     CDS             complement(7178..7369)
                     /locus_tag="ACHJ12_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990305.1"
                     /translation="MQRRGLWLVFPPSLTAEYLTDRFFDCASYWRINPFELLNMPISE
                     IPLLVSQANRIEQEKRTHG"
     gene            complement(7320..7595)
                     /locus_tag="ACHJ12_002536"
     CDS             complement(7320..7595)
                     /locus_tag="ACHJ12_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4990306.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQNSVDQMSPADLNAAAWLVAGFPSKPDG"
     gene            complement(7595..7951)
                     /locus_tag="ACHJ12_002537"
     CDS             complement(7595..7951)
                     /locus_tag="ACHJ12_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4990307.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(7951..9447)
                     /locus_tag="ACHJ12_002538"
     CDS             complement(7951..9447)
                     /locus_tag="ACHJ12_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4990308.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTMIASSIQDAINAVPTLPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMQPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(9431..9601)
                     /locus_tag="ACHJ12_002539"
     CDS             complement(9431..9601)
                     /locus_tag="ACHJ12_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4990309.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(9610..10170)
                     /locus_tag="ACHJ12_002540"
     CDS             complement(9610..10170)
                     /locus_tag="ACHJ12_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990310.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            complement(10167..10673)
                     /locus_tag="ACHJ12_002541"
     CDS             complement(10167..10673)
                     /locus_tag="ACHJ12_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990311.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(10648..11058)
                     /locus_tag="ACHJ12_002542"
     CDS             complement(10648..11058)
                     /locus_tag="ACHJ12_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4990312.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            complement(11055..11378)
                     /locus_tag="ACHJ12_002543"
     CDS             complement(11055..11378)
                     /locus_tag="ACHJ12_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4990313.1"
                     /translation="MLLTMEEIKAQLRLDEDFDTDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVSPY
                     RYFPQ"
     gene            complement(11381..11581)
                     /locus_tag="ACHJ12_002544"
     CDS             complement(11381..11581)
                     /locus_tag="ACHJ12_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990314.1"
                     /translation="MILKQDLKWSPDGMRVEVIQAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            complement(11630..12835)
                     /locus_tag="ACHJ12_002545"
     CDS             complement(11630..12835)
                     /locus_tag="ACHJ12_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4990315.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(12850..13500)
                     /locus_tag="ACHJ12_002546"
     CDS             complement(12850..13500)
                     /locus_tag="ACHJ12_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4990316.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAYA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(13478..14719)
                     /locus_tag="ACHJ12_002547"
     CDS             complement(13478..14719)
                     /locus_tag="ACHJ12_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4990317.1"
                     /translation="MFFSGLFQRKSDAPMTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKLRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPIYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(14719..14901)
                     /gene="ymfR"
                     /locus_tag="ACHJ12_002548"
     CDS             complement(14719..14901)
                     /gene="ymfR"
                     /locus_tag="ACHJ12_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFO4990318.1"
                     /translation="MNMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(14913..16646)
                     /locus_tag="ACHJ12_002549"
     CDS             complement(14913..16646)
                     /locus_tag="ACHJ12_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4990319.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGNIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTAGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPITIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(16643..17137)
                     /locus_tag="ACHJ12_002550"
     CDS             complement(16643..17137)
                     /locus_tag="ACHJ12_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO4990320.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(17263..17613)
                     /locus_tag="ACHJ12_002551"
     CDS             complement(17263..17613)
                     /locus_tag="ACHJ12_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO4990321.1"
                     /translation="MPPRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRERVLKRDKGLCQLCLRGGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WLCHKAKTARERLK"
     gene            complement(17753..18331)
                     /locus_tag="ACHJ12_002552"
     CDS             complement(17753..18331)
                     /locus_tag="ACHJ12_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990322.1"
                     /translation="MLTVLFYVLLCMAAVHFIYERILLPSVRLHYRNKLFELRDIVRN
                     QIISNKSKDDVQAAELVHEALNNAINRLHLMTLPNRVRAQRRLSANPEIQARIKREVE
                     LFQKCDNGKLIATIRESAKILDKVLLFNSLMLIIYSLPIMLTIWSVAKVLQTANQFLE
                     LLTEKQSLEQAVLLLPDRQVAKLVAEENYAYA"
     gene            complement(18348..18728)
                     /locus_tag="ACHJ12_002553"
     CDS             complement(18348..18728)
                     /locus_tag="ACHJ12_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990323.1"
                     /translation="MGKSKGKGGKSGKGQHSVSFAQAWRDVMLKAMTTGQLVPITISI
                     VLMIAAWRMPSEELGILAHRIIDGLADHSLIGYGLCVIVILAWSWHASMMRRCFSVEA
                     RRIGAEKTLHQQARTQVPLGSSDN"
     gene            complement(19487..19924)
                     /locus_tag="ACHJ12_002554"
     CDS             complement(19487..19924)
                     /locus_tag="ACHJ12_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4990324.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKATSTI
                     ADMQKRQHDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLD"
     gene            complement(19921..20397)
                     /locus_tag="ACHJ12_002555"
     CDS             complement(19921..20397)
                     /locus_tag="ACHJ12_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4990325.1"
                     /translation="MQNLNPQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKEAGGMVREIEV"
     gene            complement(20384..20689)
                     /locus_tag="ACHJ12_002556"
     CDS             complement(20384..20689)
                     /locus_tag="ACHJ12_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4990326.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(20913..21092)
                     /locus_tag="ACHJ12_002557"
     CDS             complement(20913..21092)
                     /locus_tag="ACHJ12_002557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990327.1"
                     /translation="MSNTRCSNESCNKEFIYWEHSGGYPGGKDKEPIICPYCGHTNGY
                     EMTSGLISSKKIEEQ"
     gene            complement(21296..22048)
                     /gene="ydfT"
                     /locus_tag="ACHJ12_002558"
     CDS             complement(21296..22048)
                     /gene="ydfT"
                     /locus_tag="ACHJ12_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4990328.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFTLFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKQSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRRVTR"
     gene            complement(22062..23051)
                     /locus_tag="ACHJ12_002559"
     CDS             complement(22062..23051)
                     /locus_tag="ACHJ12_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4990329.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKLADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEMYGSQLELIFRFIDRALAIGVLA"
     gene            complement(23059..23868)
                     /locus_tag="ACHJ12_002560"
     CDS             complement(23059..23868)
                     /locus_tag="ACHJ12_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4990330.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKNRPKYWLSNK
                     QTCELIEQLFTEGGIPPMEQNQPVSVINGGNNQGTYVCKELVYAYAMWISSSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(23888..24277)
                     /locus_tag="ACHJ12_002561"
     CDS             complement(23888..24277)
                     /locus_tag="ACHJ12_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4990331.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(24274..24600)
                     /locus_tag="ACHJ12_002562"
     CDS             complement(24274..24600)
                     /locus_tag="ACHJ12_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO4990332.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVWIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            complement(24597..25250)
                     /locus_tag="ACHJ12_002563"
     CDS             complement(24597..25250)
                     /locus_tag="ACHJ12_002563"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4990333.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQIPTGAFFSGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            complement(25250..25744)
                     /gene="yfdN"
                     /locus_tag="ACHJ12_002564"
     CDS             complement(25250..25744)
                     /gene="yfdN"
                     /locus_tag="ACHJ12_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4990334.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            complement(25741..26682)
                     /locus_tag="ACHJ12_002565"
     CDS             complement(25741..26682)
                     /locus_tag="ACHJ12_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4990335.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(26672..26851)
                     /locus_tag="ACHJ12_002566"
     CDS             complement(26672..26851)
                     /locus_tag="ACHJ12_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4990336.1"
                     /translation="MRQVNRWFKDHYGVLVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(27027..27584)
                     /gene="ymfL"
                     /locus_tag="ACHJ12_002567"
     CDS             complement(27027..27584)
                     /gene="ymfL"
                     /locus_tag="ACHJ12_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4990337.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(27607..27855)
                     /locus_tag="ACHJ12_002568"
     CDS             complement(27607..27855)
                     /locus_tag="ACHJ12_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4990338.1"
                     /translation="MTPEQLALSEAIALAGGQSELARKLTASSGHLVKQQHVWNWLNR
                     EKRPPAKLSIFIEKTTGISKEKLRPDIFQKIKDSSDEK"
     gene            27991..28677
                     /locus_tag="ACHJ12_002569"
     CDS             27991..28677
                     /locus_tag="ACHJ12_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4990339.1"
                     /translation="MKTLAERLKIGREKAGMSQAQLAEKIGLSQQSVAKIENGETLQP
                     RKIKEIAKVLGVSQKWLQLGIEDNASIPDLVVKEAESTALDPDIFVNIPVLDVELSAG
                     NGCLAEIVESAIDWFPLRRADLRKSGVCASNAKIVQIWGNSLLPVLNNGDLVAVDISQ
                     TVPIRDGDLYAVRDGVLLRVKILINLPDGGLILRSFNKDEYPDEILTFEDRRARIHVI
                     GRVFWSSRTW"
     gene            complement(28660..29550)
                     /locus_tag="ACHJ12_002570"
     CDS             complement(28660..29550)
                     /locus_tag="ACHJ12_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFO4990340.1"
                     /translation="MEDALYAFNYTQNRDKLFANLISIIDGIIADGVVREEEVLYLDT
                     WLLEAKQIINNGVIKSLSARVSDILADGIITSEERDDLKNSLLQIQREILDIPEIDFY
                     SKDVDVHLLNGLCKGLIADRNLTQEEIRYLNWWLEQNGALKNNYPGKKLYALVKEILK
                     DGVITEDESLTLHKALVDFTGCDLESGVVDGLATRLPIDVGASIELEGKTYCLTGTFV
                     AGKRAVVENLIKNAGGNISSGITQKLDFLVIGTLSSRDWKFSSHGRKIEKAISYRDDN
                     GAKLKIISEEMLFDALPSSR"
     gene            29756..30064
                     /locus_tag="ACHJ12_002571"
     CDS             29756..30064
                     /locus_tag="ACHJ12_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990341.1"
                     /translation="MNEQDLKHVIALLLEDAKRLQQIEPNAGTEARILLAKQALKTCG
                     AQDPDRTKFMNFMANTITPLPCNGERVSRVYHDTMVKALRIELDGLRRKIVMNKIVAN
                     "
     gene            complement(30002..30343)
                     /gene="ymfI"
                     /locus_tag="ACHJ12_002572"
     CDS             complement(30002..30343)
                     /gene="ymfI"
                     /locus_tag="ACHJ12_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfI"
                     /protein_id="HFO4990342.1"
                     /translation="MNQFYVHVRLFEATAEQTKKFEELMLNFLYQKTIKESDDSCCRL
                     IPEGYILKSTMNCQQILDQTFSIANSAGVDANIFVCKFEQSACLLPSASLVGNDFVHH
                     DLTPKPIKLDS"
     gene            30623..30985
                     /gene="yfdP"
                     /locus_tag="ACHJ12_002573"
     CDS             30623..30985
                     /gene="yfdP"
                     /locus_tag="ACHJ12_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO4990343.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            31051..>31819
                     /gene="yfdQ"
                     /locus_tag="ACHJ12_002574"
     CDS             31051..>31819
                     /gene="yfdQ"
                     /locus_tag="ACHJ12_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO4990344.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKAAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLL"
BASE COUNT         7395 a         8968 c         7551 g         7905 t
ORIGIN      
        1 gttttcgaga gctgttttca ccgtgccatc cgatttgata tcgccaaacg gattcttgcg
       61 gcttaacagc agcgcacgaa gcgcggtaag cagctggtca tgccgcccct tctccaggct
      121 ggcaccggat gcctccacaa cgctgcaaag ctcctcctgc aacatgtcaa agtagtcatc
      181 atccagatcg gtggcaggcg tgccggtctg ggggttacca cgggtaaaac cgttcttacc
      241 cgcgccgaac ttatccttct gcgcggtttt cgtgtctata cgatgcatgg attactccgg
      301 atatttaaaa atcacatagg tatgcgacgg gcagagtttg ttaagcacac actcgacaac
      361 ggtgtcgccc catatacgca gcgcggaatc acagggatcg ccacatgtca tccaggtggt
      421 gttggtggcg gctggcatgt tgacctgcca gtaataccgc cattcaggcg cattcaccgc
      481 gtcagtacag gccgatgagc aggtgaatgt gcttttgtcg tatcgcgtga tgttggcatc
      541 cggtctgccc agggcagcaa gctgtgcaag ataaaaattc tcgttgatgc cgcccgccag
      601 gttaaccttc gcatccagcc gttgctgacg ctggcgaagg gtctgtgtcc ctgtaggaat
      661 acattcatcc ggcaggccgc acagacgctc ccagcgattt atcagttcag tggtggtgcg
      721 cggatccagc tcccgcatca gggcatccgc acgctgatga acgcgggtta atgacggtgc
      781 cgcaccggca atcgccggat cgctggctga ccacgccgga ccgggcggca acagtgctga
      841 caacagacgg atgtaatcat cgtttgtcac gtccatgaaa tcgcccccag aaccgccagt
      901 tcatttttcg caatggtgat attgtccgcc ggtgcaagca actgatggct gtattccccg
      961 ttcgcaccgg aaatcgcttc actaatacgt gacaccttca gttctccctg cggataacca
     1021 tcacgcagca ggaacgaacg caactccgcg gtgatggcag cccgtatttc cggtgtgtcc
     1081 ggcgtcacac ggatatgaaa atccaccgta tgtgccaccg gcctgaacac atacaaatca
     1141 gagcctgcca ccggggccag tggcccgata tgttgtcttg ccgccgtttc cgttgattct
     1201 tccggaatgg gattaatcag gtcactgctg gcaatcatca caccgacagt ccccgttccc
     1261 atccagtgtc ggtatgtcca tgcgcgggta atgccaggca cttctttagc ccagacgaca
     1321 tagtccccgt cagccccgcc ctgcggcgtc cagtaatacc gctcaatgac gcgggcgcgc
     1381 cacgtttcca gctcttcagt atcaaatccg cctgtcaggg tgtcagccac accggaagac
     1441 ggcagaccat tcaccggcgt gaccaggatt aatgccgtac cgtcgtcagc gttaccgacc
     1501 gcacctgcac ttgagcaggc gatcggcacg cgcaggacac caccggagct gattgcatcg
     1561 tcagttgtcg tgtactgcac caggtcatcg cgctgaataa cactcccggc agtcaccttc
     1621 aggccatcgc tgacaccttc ccagcgcata tacccgctgg caaccgtggc tcccttgcgc
     1681 ggacaccgtt tcatcgcagc atgtcgcgcc agccaggact catcgcacag gtcaggcagc
     1741 atattcattg ccagataatc gatgtaaccg tagaccgtat gcagcgccgc cgcatacacc
     1801 tttgcccgca cgtcttcatc catgcgccgg agcgtgtcgc tgacgtccag cctggcgaat
     1861 aaatcgttac ggagcatact gatattttct gccagcgtcg ggcgctgaaa ttcactgtcc
     1921 gccatgcgtt atcgcactcc acagatcatc aaaagaaatc attaccggtc cgtcacgacg
     1981 ccagagagtg atactgttac ccagttcatt aatcccggtg cgacggatat ccagatcaat
     2041 acgggacacc acgccgtcat caatcatcca ttgcaggcat tcgcggatat aaccccttac
     2101 cgtctgcacc agctgattgg tcagtttgct gcgctgaagc agccacagtc gggagccgta
     2161 acggtcattc tgtaccgcag gccaggtatc cccccaccat cccatcggga cgtcggcgtt
     2221 gtcatcaggc tccgcccgcc gccaggtaaa cagggaaatc accacggcgc gggtcagcgg
     2281 atccagcggt gcgctggcgc aggtgcgttt accgttcacc gtcagccaca gttccatcat
     2341 gcctccatcg ctttatcagg tttgtcggtg ttactgccct gaccgttctc tctgtgacga
     2401 tgcccgttat aggcaagccg catcgctgac atggtggtgc cgccggagtc gcacaggtct
     2461 ttcacctgtc ctgtcacttc caggtccatt tcaaaacgtg cttcaggtgc attgcgaaac
     2521 gtgatcgttt tacctgcacc atccaccacg atcccctccc gggtcagcgt cacggactgc
     2581 ccctgatcgt catagaccgc cacctcaccc gtctgcagcc ctttcaggcg gtagcgccgg
     2641 tccgacaccg taacaactac cgcatgagaa cggtcaccat ccggaaacaa caccaccgct
     2701 tccgcaccgc tgtttgccct tgcggtaaaa ccgtagggtt caagatgttc aaccccggct
     2761 ttgggttcac cggcaatcag ggacacatcc acggtctgac atttcgtggc ggcactgatg
     2821 cttttcacca cggcccgccc aatcaggccg aggagttgtc gctgcatggc ttcaatcgtc
     2881 ctcatcagaa cgggtcctcc tgtactctgg cttttttctt tttccgcgcg ccgggggctt
     2941 cgggttcagg cagataagca tcaggtgggc cgacacggat ttccgtcagg gtgccgttct
     3001 ggtcctgagt aaacgtgact tccgagacaa gcagttcggt attgtcgaaa ccacagaccg
     3061 gatcaaagac aatcacccgc tggttgggct gccacagcgt accgttaccc tgtcgccagc
     3121 cctgcaccac ataggtggtt tcatccgtcc gcgccgcccg ttgtcgggct tcaaagtcag
     3181 cacgggcaat acagcctgcc cccgtggcct gccctgtctg cctgatatac atcggacggt
     3241 aacgggaaat aaatgcgtcc tctgtgcggg cccgcagcgc ggtggtggtg gcctcaccga
     3301 aatcatcgtc gtttccggca cgctgccccg ccacctggta aacagaaaac cgctcccgga
     3361 tactcttctc cgtatcgcag gaaaggatgt tttccccaag taccagcgca gtatgtgccc
     3421 gcgttgagcc aataccgcca atcaccagcc tgccgtgcgg gtcgtcgtaa gccagtgcct
     3481 gctgctgacc gagtattttg ttgattacct caatcaccgt ttcaccgtga tcaggctgga
     3541 cgtcaggaat aacacccgac ggcgcaccgt tgttcaccac ctcaatgccg aaaggcgcag
     3601 caagcgcctg cgcaatctgt accagcgatc gtccgttaaa ctgtgtcggt ttggctgcac
     3661 agtcaatcag gtcagcggtc agactgcgtc cggcaatacc ggtgctgacc gaacgggcat
     3721 cgtaacgaac gggcgtcgcc tccacccagc cggtgatcac cagctcatca ccaatcagca
     3781 cctccacttt tgaaccgttt ttaatgcgcg gctgaagcgt ggtgataccc tcatctcccg
     3841 gccactggcg ggtgatctcc acactgaaat cccgcgccag ccgttcaata ccggcaccga
     3901 tgcgcaccga tgtccagcca ttccactccc ggccatttac ccgtagcgtg acattgtcgt
     3961 tcattgcact ggcaccttca gagggatcac cggcacaaat ccgggatgcg taatggcatt
     4021 acgccggata atgtccgcgt cacgcgccgc gttatcaaac caggtcgccg ccagcaccag
     4081 cgcgggtaaa acctcatccg gtgtgcgctg aataatccgt gcagactgtt caaggcgcgt
     4141 gttgatatcc gcattcagat ccgctttcac ccggcgcagc gccagaaaca gcgcatcact
     4201 ggttgttcgg gacaactcct tatcaattgc cgtattcagt gtgtcgcgaa tgtcagtcag
     4261 ttcttcccac gtcggcaggt caaccgtgtt tttcaccgcc ggtgcattgt tcagtgccgg
     4321 atgcgtgacg gaaggccagc cagtgctctg cgcaggtgtt gttgcctgcc ccactgtggc
     4381 attctgcatc accgcggaag ttgttggcgc aggcaatcgg gtgacggcat acgccgcttc
     4441 gctgattgcg gtcgtacgaa gggtgctggc aaccacgtta cgctgctgcg tcgccgtagc
     4501 ggtggtttta ctgtccgttt tccagacgcc gcgcggttgc agatcgctgc cgaggctgac
     4561 accggaaagc gttttgatca tggtgaccag gtcgctggcg ttaccataaa ggcgtttccc
     4621 ggtacgccac attttctgca cctgctcaac gaaatttttg cctgacgatg gcggcggcag
     4681 aagtaccgag atatccccct gcaacagcct ggcggcatcc gatacggcag aatccaccac
     4741 tttcatcgca tcagaaacat acccaagcat tgtgctggca ttaccgacga cgtcgttctg
     4801 cacaaaatct gccacgccat cgatactgaa accactgaag ctgtcactga tgcagtcatc
     4861 cagtgcagaa caggatgaca tcagcgtctg cgccgtcgcc gcacctgaag tggggtaaga
     4921 gagttctccc gcttcgacaa acttcaggtc aaagcggaca atacgccctt cactcttcga
     4981 tgtgctgacc cgaacttccc catcaacaca gactttcagc tcaccgtaag tcggatggac
     5041 aagcgtgccg ggaccgggtt tattcagcgc gtcaatcagg cgatcgcgct ggtcaaagca
     5101 gtcatctccc accacataag ctgtgatgga cgggcggaaa gtgattttcc ccaggtcttc
     5161 ggtatagggt ttgtcgcggt tcgggtattc gtgcgtttcc acacgacgac cggttcccgc
     5221 actttcttct tcaaccttaa acggcacacc gcgaaatgac gcgtcctgaa gtctgtcttt
     5281 ccacgtcata taaactccgt acataaaaaa tcccaccgga gtgggactca ttaacagatt
     5341 aatttttcat tacctgccaa agcgcgtata gccaacatca tggctgacat caaaaccgct
     5401 ggatcgcgtt tccataaccc gcatacccgg aggcgaattc acaaaagaga ccttgatctc
     5461 accatcaact tttggcgcag aagctttgtt aatcatgaag ggattcgggc ctgtggcacc
     5521 ggaggcgttg tttgactgag ccggatccac caccggataa ggtgtgtatc cccgcgccgg
     5581 tattcccgtc ccataagcat cataagcacc cgcgccccac tgcgcagagt taatggcatc
     5641 gaccgtgtca ccggaactgt cggtaaacca ctcaataatt ggcttcagct tgtcccacat
     5701 atcctgaaac cacttaacaa ccggtcccca gttattgatt accatcccca gcggcgacca
     5761 ggcaaaaacc ttcttcagaa gttcccggcc agcctcaaaa taaggaccaa tggtttccca
     5821 gagcttcttg aaataaggtc ctacaacatc ccagttagtg ataattaatc ccgcagccag
     5881 ggctatcgcc gtcgcaatca ttccaatcgg cgtcatcgac atgatcctgc tgacaatact
     5941 gatggcaccg ccaacgccca tcaatcccag tttcagaatc gcaagaccgg cagcaagccc
     6001 gacgacgccg cgaataaccc ggggattttc atccgcaaac ttcgtgaatt tttcccccaa
     6061 ctcccccagc cattgcgtga tatttttggc gtcaccagaa aatgcgccgc caatagccgc
     6121 aaggccgtta gttgcggtcc ccgtcattgc ctcccacagg ttggacagcg taccaagctg
     6181 ggcctgaaca cgtttattca ggctggcctg tttattcatc ttctgctgga tctgatcgta
     6241 gccatccttt cctttatcga tcagtgcatt gaccacctga agggtttcgg catcatcacc
     6301 aaatattgcc ttaagtacac ctgttcgctt aacgtaggtc agttttcgca gctttgccag
     6361 ttgcctgaac atgttatcaa gaccgccaaa acttcctttg ccgtcagtaa aatcgagctg
     6421 taccccgagt ttctggcggg ccatgacttt attgacgtcc ctgattttct taacgcttaa
     6481 tccggactgg ataacttttc gcagggcatt acctgccgac tccccgttca tccccatctg
     6541 atccatcatg acactgatgg gggcaagact ctgtgcagcc tgaagaccgt ccttgttcac
     6601 catcttcaga acagagctgg ttttagtgaa gaatgacagc atgttggtgt cgtcaacgcc
     6661 cagataaaac gccttctgaa ttgtgtcgaa cagccccatc atgtcttctg agaccgttcc
     6721 ggtagcatcc tgcatctttg cggcaaactc ggcagccgct tccggtgttt ttttcagttg
     6781 taccgcaaga taagctgtcg ctttacccac accgccaaga atgttttctg ccgggatccc
     6841 ctgacgcacc agcatctgca tcatgttctg aaaatcagct gttgtaccgg gtagctggtt
     6901 acccaggcca atagccagtt tattgatgtc ctgaaagcgc tttccaacct cgccgttcgc
     6961 atccatcatg gcgactttca gcccggtggc ggcgttttcc tgatcggcat aagatttcag
     7021 ggaaagcgtc agacccgctg ccagtccgcc accaagcgcc agcccaccct gtgacgcttc
     7081 ttccgcctgg cgtttaaatc cccggatttt cttttgcatt ttcgacagcg cgggagaaag
     7141 cctgtcgaca ccggtgatca acgccttaag ctcaaattca gccatgtgtg cgtttctcct
     7201 gctctatcct gtttgcctga ctgaccagca agggaatttc actgatcggc atattcagca
     7261 attcgaaggg attaatgcgc cagtagctgg cgcagtcaaa gaagcgatca gtgaggtatt
     7321 cagccgtcag gcttggaggg aaaaccagcc acaagccacg ccgctgcatt caggtctgcc
     7381 ggagacatct ggtcgacaga gttttgcggc actttcgcca gccgcacaat gtatttcgat
     7441 accacatgcg ccagaagtct gacggactca tcctgattca tctggtaggg ataccccagc
     7501 tcgcggacat ctttcccggt gggctcatca aactccagta cggagagtgt ctcgccatga
     7561 gcggtaatcg gtttctttaa ctcaagctct ttcattactg gtaatcccct tcttcaccgt
     7621 ggaactcaag atcaaccgtc ccttcttcgg cattatggtt cgcttcgccg tgcagccagg
     7681 cagacgacag tacatagacc tgaccgttcg ccagctcggc agtgatggtc atctcatcag
     7741 acgaggtgat tttgctcacc ggaaaattct tcggcacctt gaaggtccct ttaacataag
     7801 gcgcacggtg agtttccttg cggtccactg aaccgtccag gccgatgatg tcatcattga
     7861 ccgtcctgtt catgggcacc tcaatgccgc cggtcagcga tagctgctga ccgtcaattt
     7921 tgaaataaca ggttcccccg atacgggcca ttatgcggac tcctctgaat actgaagacg
     7981 gaactggtta accacggcaa agacacgcaa ctggttaaca tagtcaggcg ggaacagcgt
     8041 gttcaggcgg ttcggatcgc tggcatcacg ctccacaacc aggtactgct taaaaagttc
     8101 gtagttttcc acgatccccg cacgctcaag ctgacggtag gttgccagca gttccccttt
     8161 gatcaccgcc ggggtgacaa tcgcctgacc gggaccaaag cgggtaccgt cgctggcaag
     8221 cttgtgacgc ccgtacttac tggtaatgac ggatttcagt ttgcgcagta catacgcact
     8281 ggtatgcagc gtctcgctgt cgaggtagct gttatccgca accccgtaag cgtttttcct
     8341 gtacgtggtg acatcacgct gaatgcgcag taccccgctt tcgacatacg ccgttgccac
     8401 gccatgagac agcaaggtct gttgttcggt catcgtgaac cgtttcccct tcggcgcagg
     8461 ctgcataccc accagctcac cggtctgcgt gggacgtgcc ggatcgttgc ggataaacac
     8521 cgctgcgcgg gcggtacggc ttgccgccag ctcgtcggca ggcgtctggg tgtctttttc
     8581 gtaccccgcc agggtaatgt gctgctggtt aaactggtca cctgcggtca ccagttctga
     8641 cagcgtgccg atctttgccg tatacacatg accatacagc tgacgcgcat agctccagcg
     8701 accgctggta tcgttcatct cggtcaccag cgtgttaacg gaggccgtgt cgttgaacgg
     8761 caggccgata taatcaaacg gctcatccgc cattgcagcc accgcgccgg tgagaaccgg
     8821 agcacccgtt ccggcggtac ccgtcgccac ggcaatctgt acgcccgctg gcagcacttc
     8881 gcccccacca aagccgtagt aattgaggct gacaggaatt tcattcccgc aaagcccctt
     8941 atgacgcgcg gtcagtgtga ccacgcctgc cgaagatgaa gccgtaaacg gcagggtcgg
     9001 aacggcattg atggcatcct ggatactgct ggcaatcatc gtgacgttat cgccgttagt
     9061 caccggtgcc tgcacgcggg tacgtcccac atacacattc accgtgccgg tttcggttgc
     9121 cgccccggtc accgtcagcg taaccgttgc cgccgcgcct gtggattcag gaacggcaat
     9181 cacatacagc tcgccaaacg ggtcagtctg gcgataagcc tcgaccatac gcgccagctg
     9241 acttcccgca ccacaaatct ggcgtgcata gtctgccgac ggcatcagta ccagactgtt
     9301 ggcaacaatc tctgcaccgt tattggcatg accaatcagc agcgatgctc cgctgtcctg
     9361 tgcagtattc gccgcctggt tatccatttc cgcataaaac aacggaacca gcgtattcga
     9421 cggaatggtg ttaaagctta tcgtcatcgg tattcacctt tttattcacg cgccggatat
     9481 cacccgctgc ttcacggcgc agccagtagt tgttctcgtc aacatttcgc ccttcggcgg
     9541 gcaaaaggtc gccgcgggca ggatcaggaa ctgaccgccc tttaacaggt ttgacaaaca
     9601 tgaggatcct caggaaggaa gggttatttc ggtgtgatgt tcgatatcgc cgtcaggccc
     9661 gttaccgggc tcgagataat caacatcaat cgccagcgtt tgcagttcat ccagactgtt
     9721 cagatcatcc tgctggcggg tatcgtcttc agtcagctcg ctgatgaccg aaaaatcgaa
     9781 ctgataaatc agctcatgac gattcagatc cagcagcgtg ccgccgtcat aggtaatcgg
     9841 gttaccgcac gcctccgggt tccagcccag cagagcctta aagagcatct gccggacatc
     9901 gtccaccaca tcatacgagg caaactgacc gcgctcatca cgcccgttac tcagtatgac
     9961 aaccacggag aagccctctt tcagctcctg ccagtagtcg gtctggcttt tgttttctcc
    10021 cggagagtca tcacccggta ccacatacgc cgccgggagt ctcagctttc cgacctccgg
    10081 cagatttttg aactgggccg cgcctgccac ccggttttca aaatacggac agcgggcacg
    10141 cagtgcagca ataacaggcg tcagtttcat ctgtgtcgtc gctccggctt cagtgattta
    10201 cgcaattccc gcgccagaaa atagcgtgtc caactgcggt tcttttcaag cgtttccacc
    10261 atgaagttat tacgtggagc cagtcgccag ccgctgccac cggatgcacc acgatgatgg
    10321 ctgcgacgac gctttgcccc tcgcctcacg ccatagaaca aaaaagccgg ataaaaatca
    10381 ccggtgatac ggcggtttcc ctctccatta cgctggttag gggctatacg tgccataaaa
    10441 ccagggcgat gtttactggc tctgggtacc atgtaaccaa tcgaacgagc caggcgtccg
    10501 gtctgataac cggggttttc acccggtgcc gaccgcgcac ggcgcatcac cagccgacgg
    10561 gcatcacgca tatgacgctg accaatcgtg acaaacgccc gccggacacg ggcgcggtta
    10621 aagcgcatct ccgcgggctg ctgaaaatca acgtgcaaaa aggaagtcgt cattgttgcc
    10681 tccgtgactc tgcctacatt cgcccagctc cgtacactcc agcagcagaa agcgccgcgc
    10741 cccgttcaga tcgcgctgac gtttcacccg gtacacactg tcaccgcaga ccacctcata
    10801 atcagcggtg atcccccggc ggtaacgaat ggtgatgtaa tgggtgatgg cgtccccggt
    10861 ctgcgcggtt tcctgccagg tggtggcact ggtctggata accttcgccc atgtccggaa
    10921 cgtaaccggg tattgaggct ccacgccaaa gttatccgcg ggcatatcca cccgcaggcg
    10981 gatcaggacg cgtttattca gttcaccggg gtccggcaga atgtaggttg cgctggtctg
    11041 cgcctgacga attttcattg cggaaagtac ctgtacgggc tgacaagcca gccaaaactc
    11101 tgcggcatgt cgagtttctc cacttccgta accgacgagc ggttttcgta aaaatggctg
    11161 ataagcatca gcatccccag acgaatatca tccggcaggt gcagcccgtc cggatcgctg
    11221 tccggaatgg tttcatctgg tgcatagagc ttccggttca gatacgtttc cgtccgcttt
    11281 tgtgccgcac atgccagcag ttgcagatgg cggtcatcag tatcgaaatc ctcatccagc
    11341 cggagttggg ctttaatctc ttccattgtc agaagcatac tcagccctct ttactggtcg
    11401 tggctttttt ctcttttgcc gctttactgc tttttgcact gattccgcgc tctgctaacc
    11461 cggcctgaag tgcaatctcc tgcacccggg caggaagcgc cccgtcgtca tactcaccgg
    11521 cctgaatgac ctcaacacgc ataccgtccg gtgaccattt cagatcttgt ttcaggatca
    11581 tgattcttca ctcgtcagaa caggggcgcg gttccgcgcc cctgagtgat tacgccgctg
    11641 caatcttcag cagtttgatg gcctgcgaat cgaccagcat cccgccggtg cgcttggtgg
    11701 tataaaaacc gacaaacggt ttattggtgt acgggtcacg cagaatgcgg gtgccgatac
    11761 ggtcaacgat ggtgtaaccc cgtttgaagt taccaaatgc aatggctttc gcatcagcgg
    11821 cgatatccgg catctgttcg ttttcagcga taccgtaacc cgccagagag gacggctgcc
    11881 ccagttccag ccccggacgc cacagatagt taccctcggt gtctttcagc agacggatgg
    11941 caaacaggct gttgttgttc atcatgaact tcgcgccagt gcggtgtgcc ttacgcagcg
    12001 tgtaaatcag tttgataatg gcgtctgcgg tcaccgcggt cgcttcgccg gatacaatat
    12061 gctgaagttt gccgaacgcc cggaccttgt cggtttcatc agtggattca tacgccagga
    12121 accctttcgg cttcttggtg ccatcgcctg aggtaaaggc aatttcttcc tgttcggcaa
    12181 attcggttgc cagctcgctg ttgatccagg cctccacgtt gaagaaggca tcgtccagca
    12241 ttttctgggt agcctgcggg ttgccgtaga tttcccccat gagaggttca atcagctcca
    12301 gtctggaggt ggcagtctgg gatcgcgtat ccgtttcccc cacccatccg gaagccgtac
    12361 cgcccagatt caccagtttt ttgtagtcgg aaccgccaac ggtgatcacc gtggcttcct
    12421 gacgcatcac cacttcatct ttcagcaggt taagaatgtt gcgatccagt tcttccggca
    12481 cggcgtagcc accgtcttca tcggtaccca cctgcaatgc cttacgctcc agatcgcgca
    12541 gaccgtcttc acggccttta cgcaggaagc ccacaaacgc ctctttatgc tcggtggcca
    12601 gtttattttg cgctccacct gccggacgtt tcagctcaag cagctctttt tcaaggtcgc
    12661 ttttgagatt ttccagctcg ctgagtttcc cgttcagggt ttccacctgc ccggcaagct
    12721 tgcctttttc ctgctcaatc gcatccacgc gcttgtcgtt ctttgctttg aagtcgtcaa
    12781 acttctgctg cagctcctgc gcgacctgtt cgacatcttt aatatcaacc gccatcgtat
    12841 ttctcctgat tagaagttca gatttttcag tgcattcagt gcagagccca catcctcagc
    12901 gtcgcgcagg gacagtgcgc catagccccc ggccatgaat gctttggcct gggtacggga
    12961 gagtccgaca tcacgcagga ctctttcgat ttttttctgt tcggggattt ccccgcgggc
    13021 cagtgcgttc ttgacgtcgc tgatccgcgc ctcgtcgtta gacgggaacg tcaccaagct
    13081 gacttcccag aggtcgattt ctttcagcag aaaggcttct ttgctccggt cgtattccca
    13141 gtctttcagg acgtacccaa tagaaaggcc ggttaacgaa ccggccttca tgtgtgcata
    13201 tgcgcgtttt gcgaggggat catcatcaat aagcaaccgt cccctgacgt aaagcccgac
    13261 atcgtcttcc ttcatttcgg tgtaaacacc gatgggttca tccatgcggt gctgccagag
    13321 cagcgcaggt aacgcttttc tgtcactcca cgcccgcagg gaagcagcaa atgccccgga
    13381 catcaccaca tcatcgtggc tgtcctttac accaaagacg gagccatacc cttcaaactc
    13441 accggagtca ctgacagatt tcagactcag cggtacatca agacgttgtt tcgtctgcat
    13501 tggcgttatc cttctgctta ccggccttac tgccatcgga gggtttcgtg gtcatgttca
    13561 tcggtgtgag atagacatca ccaccgggac gcggattcat atcttccagg tcgcggcagt
    13621 cattgggaga gtaaattccc cagttgatcc cggtggcgta ggcttcaaaa cgggacttca
    13681 tatccccgcg cagtaacgcc ccggcgttaa atttggcgta ataaacgccc tgcttacttt
    13741 ttcgtaccag tccggtgttg atccgctgtt cgatgcgggt cagatacggc accagtgaat
    13801 agttgataaa tcccagcccc agctcttcaa tattgttgaa ggtggcgcga tcggtgttct
    13861 gcaccatgtg caacggcacc cggaacagac gacagatttc ttcaagctga aacttgcggg
    13921 tttccaggaa ctggctgtcc tcggcgttca gcgccatcga cttccagtcc agccccatct
    13981 caaggatcat cgggcggtga gcattaccaa gcccggtgtg acgctcctca aaatctttct
    14041 tcaggcgctc ataagcctga tctgacagcg tctgctctgt acgcaacaca cccgacgtca
    14101 ccgcgccatt gctgaacagt ctggccccgt gctcttcggt cgcagctgcc agcgatattg
    14161 cctcgcgggc ataggcgatg ggattcagcc ccaccagtcc gtccagcgtc agcgtgcgca
    14221 catgccagat atcctcctgg ctcagtacat ccgtggagcc atccgggaat gtgacctgat
    14281 agatcggctc ccagctactg ttaagcttcg gtaccacaca gccgggatcg acgggcagca
    14341 gttcagccac ttcgccaaat gctttcactt tgtaggcgta aaagtttccc cgcaggcaca
    14401 gacaggtgac caccagctcc cagaactcct gcggcgtcat atagccattg ggatgcgtgg
    14461 agatcagctt atgcagacgt tcgccagtgg ctctcagctt caggctgccg ttcaggtgat
    14521 acaggttgca gggcaacatc ccgaccgact ccgccagcac cctgacacag gaaaaaaccg
    14581 ccgtcagtcg catggcccgc tggctgctga tctgctttcc ggtataggtg tcgtaggaca
    14641 acccgatagc atccgccagc tctgctggcg tggtcatcgg tgcgtcactt tttcgttgaa
    14701 ataatcccga aaagaacact atttacctcc gccgacagac gactgtgtac ggtcaagata
    14761 tcgcgccacc agccacgacc agaacaggca caacgccccg gcaacaacaa accccgccgg
    14821 gggataaatc agccaggcac catacgccag caaaagcgcc cccagcacgc ccaccagagg
    14881 cgcgagaatc agcatgttca taattacctc agttaaagcg agcggatccc gtaggactca
    14941 atgtggtcag acaacgtgtc ttctttctcg tacagcatgg ctctgccaac cgccataatc
    15001 agcgcaactg caccatcgat tttgttttcc gcctgctctt tgacgggctt caccacatca
    15061 tcgttacccg gaatggtttt gccgaccacg ttgccgatac accaggtcat gatgggattg
    15121 ccatcatgat gaaagcgccc cgattcaatt gccgcttcca gctctttcat cgggtcggac
    15181 atgttggtgt agttctgaat gatagtgatg gggttcaggt cttcatcagc aaggtcatgt
    15241 gagagcccgg tcgccccgaa ggggtcgatg ggtgactcgc tgaccgggct gattttgttc
    15301 gccgctttgg cctcctcgag gatgtagcga taatccacct ccgcaccagc ggtaacggtc
    15361 agaacgccca tttccaccca tttctgaaag cgttcggctg tccggcgatc ttcatttttc
    15421 tcgacgctgt acaccgtgtc atacggtacc cagaagcgcg gagccacact gtagtaatgc
    15481 gttttaccat caatctcgcg ggtataaagt cgcgccatac tgttcatatc cagcttacgc
    15541 gccaggtcaa aggccagaat gcacggctgc ccctcgaact gctcaagggt cagtgattta
    15601 tcctcgcagc tctgccagct caccaggttg aaatacgccg aacgcgccga cacccagata
    15661 ttgaggtgtt ttgttttaaa gacgtttgcc agacgggcgt tatttttcgc acgctgctgc
    15721 tgacttaaca aaaattcgcg ataaaccgac acgccaatat tcgggttggc tttttccagc
    15781 acctgcgggt cggtccagtc atcgccttcg tcaacggtat agatgatccc gaacagttca
    15841 tcgttgggta ccgacccgtt gagcatctcg ataacttccc gtcgcttgtc gtagcacggc
    15901 ccctcaatgt tgtaccctgc agtagtaatg gcccacatca gtggctgacg tcgcgccccc
    15961 atcccggtaa gcatcgtggt gtaaagcgca tcggtggcgt gctcgtgata ttcatccacc
    16021 accgcacagt ggggtgatga accatcaccg gggttaccga tcagcggttc aaaccgcgcg
    16081 ccatcctccg gacggttcat gtttgaggcg ttaacctcaa tcccgaacgc ttccgtcagc
    16141 atgggtgtgc gtttacacat cagtcgcgcc gggcgaaaga cttcccacgc ctgtttctct
    16201 gtcgtggcac cggaatacac ttccgcgcca aactcgttat cacaggcaaa acaatacagg
    16261 gcgacaccgg cagagattgc cgatttgccg ttcttacggg ggatttcggt atacacctcc
    16321 cggaagcggc gcagccgggt acctttattg acccagccaa acgcacagca gatcacaaag
    16381 agctgccacg gctccagcgt gatgggcatc ctcttgaatg cccactcccc cttggtgtgt
    16441 ggcaacagct gaataaattt ggcggcccgt tcagccaggt ccttgtcgaa gcggtaacga
    16501 aacgacttac ttttttccgc catcaggtca tcaagatggc gctggcaggc ctgaatcaca
    16561 aactggcagg ccacaatatt tccgcgcacg acatcacggg catactgatt ggcagcattt
    16621 acgttggggt aagatttccg gctcatgatt cgatgatttt cagaaacggg ttagtggctt
    16681 tcttctgccc cgccaggcca atcagacgct ggcggctgct ggggtcgagt ccgagcattg
    16741 cccccgtact gctcatctcg gactcctgtt cttttttggc ggtcagctcc ggatttttga
    16801 ccataccgcc cattgcaccg gtgatggtgt tgccctgtct ggcaatattt ttcacggcac
    16861 gtcgccagaa ctcgtaggcc acgcaccacc gctcaagcac cgcgaggtca gtcacgcaca
    16921 gcaggccctg accgcagagt tctttagttg tcagttgcca catgatcgta gcgagaggga
    16981 gatcttcttc agcgaaccac tccggtggct caacaccttt gatgggcgta aaaacaggtt
    17041 catctttatt cagggctcgc ttgccggggt ttccggccag cgccttgcgc gccgttggct
    17101 tggggcgacg cccggaacgc cccgccgttc cagccatatg cggcactcct ggttaaattt
    17161 catttttcgc gggtataaaa aaacgatggg gcgggcagtc cggaagacgt caggtcacag
    17221 ggatttgacc cgcccctccc ctgtggcagt gggagctggt tcttacttca accgttcacg
    17281 ggccgtcttc gccttatgac acagccagca caggctctgc agattgctgt cggcatcagt
    17341 gccgccatgc gctttaggga tgatgtggtc aacggttttc gcctcacgta ccacaccacc
    17401 acgcagacat aactgacaca gtcctttgtc acgcttgagc acacgttcac ggataacatc
    17461 ccatttcgaa ccataaccgc gctggtgtct ggactggccc ggcttgtatt gcttccagcc
    17521 ttcacttttg tggctttcgc aatagcctga cgggtcagtt gtggtatggc ggcagccgcg
    17581 aacacggcag gcttttgggg ttcgtggtgg cattaatcga ctctctttac gcttatcaat
    17641 gcataaaaaa gcctcgcatc agcgaggctc agtctatccc atgaagggaa atatagaggc
    17701 gattccgtcc attggacaag caataaaaaa ccgcccgaag gcggttttta catcatgcat
    17761 atgcataatt ttcttctgca acaagctttg caacctgtct atcaggaaga agtaaaacag
    17821 cctgctctag gctttgtttt tcagtgagta actctaaaaa ttggttggct gtttgcagaa
    17881 cttttgccac agaccaaatt gttaacataa ttggaagaga atatataatc aacattaaac
    17941 tattgaaaag caaaacctta tccaagattt tagctgattc tcgtatcgtt gctattaact
    18001 tcccgttatc acatttctga aaaagttcaa cctcacgttt gatccttgcc tgtatctctg
    18061 gattagcact gagccgcctt tgagccctca ctctgttggg caaagtcata aggtgcaaac
    18121 gattgattgc gttgtttaag gcctcatgta caagctcagc cgcttgtaca tcatctttac
    18181 ttttattcga aattatttga tttctaacta tatctctcag ttcaaaaagc ttattgcggt
    18241 aatgcaaacg aacagatgga agtagtatcc tttcgtaaat aaaatgcact gctgccatac
    18301 ataacagcac ataaaaaagc actgtcagca tagtttctcc ttagggatta attgtcgctt
    18361 gatcccagtg gcacttgtgt tctcgcctgt tgatgcagag ttttttctgc tccaatacga
    18421 cgagcttcca cgctaaaaca tctgcgcatc atacttgcat gccaagacca cgctaggata
    18481 acaatgacac aaagtccata cccaattaga gagtgatctg ctaagccgtc aataattcga
    18541 tgcgctagta ttcctaattc ctctgatggc atgcgccaag ctgcaatcat cagtacaata
    18601 cttatagtta taggcactag ttgaccggtt gtcatggctt tgagcatcac atctcgccat
    18661 gcctgagcaa aacttactga atgttgccct ttacctgatt ttccaccttt gccctttgac
    18721 ttacccatta tcatccagcc gcggttatga ttttaggtaa attctacagc tataaataac
    18781 aaaataaaac atccacttac tacatctctt cttccattag ccatatttct cacttgcaaa
    18841 tcaagctttt tttgcacaac aaggcattat tctcctcaac tttctatatg ttgtgtccca
    18901 acagctaaca aacaactatc acccacatca catatgtagg atgcttaact tttttaaaag
    18961 ttcattatca cttaactaag aaccatattt tcagacaatt atctacagct gccatccata
    19021 ctttgctgta tgcctgtata tttttctttg actacttatc gagctcatcc aagtaatcaa
    19081 cagccgcaca gataggtctc acccggtccc gccctgtgtc aattaactca accttgcatg
    19141 tcctacttgt gcgcagttcg tgatcaacgc cactatcaga catatgatta acagtttgct
    19201 ataccttgtt ggtatctttc gttgcttata tccagcgctc tgctatagcc tagatgttga
    19261 gtcctcctat tcgtaacgct tctgatcagc gttcatttgt acttcgctag taccgtatga
    19321 atatcctaga ccgaataccc tagcggtagc agtgctaaac gcagcagcta gcaagataat
    19381 catttcgata cccattgtgc tcccacatca gagtagatct cagcagattg aacagaatgt
    19441 gctaggaata acaattcgaa aaaagctatc gttcaatccg cccctgttaa tccaggcatt
    19501 gagttctgat gtattcctgt aggtaattaa cctgtgcagt tatcttgtcg attccacttc
    19561 ggaggcggta ataattgagt tcagcatctg ctgtaagtct tgggctttct ccatcgccca
    19621 tgctgctggc tccggtcgtt gactttgcac aggtggcggc gacttgcagc cgcttacgcc
    19681 cagcagaaac atcatcacgg agactttcga tagtcgcgtt agcatcagca agctcctttg
    19741 tatatctggc atcgagttct gctacatcat gttgacgctt ctgcatgtca gcgattgtgg
    19801 atgtggcctt atcgcgctgc tctttgtagg taatggcgtt atcacggtaa tgattaaccg
    19861 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gttactctgc
    19921 tcatacctca atctctctga ccattccgcc agcttctttg aattttgcaa tcaggttgtc
    19981 agccttatgc tcgaactgac cataaccagc ccccggcagt gaagcccaga tattgctgca
    20041 acggtcgatt gcctgacgga tatcaccgcg atcaatcatc ggtaaagcgc cacgttcttt
    20101 aatctgctgc aatgccactg cgtcctggct tttgggggag aagtctttca ggccaagctg
    20161 cttacggtaa gcatcccacc agcgtgaaag aagctgataa cgtccggctg ctgttgattt
    20221 gagtttgggg tttagcgtga caagcttgcg agggtgatcg gagtaatcag tgaagagttc
    20281 tccaccgaca ataacatcat aaccgtggtt acgtgtcggt tgtcgcccgt tatccgttcc
    20341 ttctgaccat gccaccatat cgaggaaagc tttacgctga ggattaagat tttgcatttt
    20401 tcacccctgt cagtcgttcc cagaagtacg tcagtgcaac cgaccccatc gcaccgctaa
    20461 tccccgctgt cgcgagaatc atgtaaatac tgaatccact ttcgatgcta atcaggccac
    20521 caataacacc ggtgaatcct gataccacta tttgagccag agcatttatc caactccacg
    20581 ttgctttact ctgcttcaca tctatcaggt agcggaccag accgccccaa cctgcaatga
    20641 tcagcaaaac gagccagaac gctccggcaa ggctctcttt ttcgtgcata tgaatagcca
    20701 atgtttcgcc gccgacaaaa ggccgggacg ttaaatgtca gaatgcaggc tcacgggtta
    20761 atttaacgac aaagcacgga gttgatgctc cccgcaagcc ttgaataaaa aagccagcat
    20821 atggctgaca acagagggtt aagcaatgtc aattcatcag gcgaaggcaa caaaaagccc
    20881 cagcaaatac cagggctaga cagatatgaa tactactgtt cttctatttt tttactggaa
    20941 atcaatccac tggtcatttc ataaccgttt gtgtggccac agtaagggca aataattggc
    21001 tctttatcct ttcctcctgg atagcctcca ctgtgctccc aataaataaa ctctttattg
    21061 caactttcat tactacatct tgtattgctc attgcatcac tccctcagta agtaaggaaa
    21121 taaatacata gcataaccaa atcattattc gaaatttgag cacaaaaaaa acccgctcga
    21181 tggcgggttc aagaagatta tcaacggtag acacacaaag cccatcgtta ggataatcct
    21241 aaccatattt tttgaaaaat gcaagaatca tgtcgccatc ttcggcgaaa atcatttatc
    21301 ttgtcactct cctcaattgt gtctcagcat atgcttcttc ctgccagcac tttgtaacca
    21361 gtttatcaat gacatctgca tatcctttgt accactgata atccgtcagg tctggtacca
    21421 gcttctggac atgatgccgc gccagtgtgg ttggtaaacg gctaaaccgg tttccattgc
    21481 aacgcccaca aatcttataa acaggcgtgc catgaagccg ggtccttttt tcatccagga
    21541 caataccttt acccttacac cctctgcacg ctgtgctgac ttctccctta ccatgacaat
    21601 gctgacatag ttcctttacc cactcttcct tgataacaga ttccccgctt ctggattgtt
    21661 tcaccacctc gcgcaataca ttatgaaatc cagtaccagc acaatgctca cagcgagcct
    21721 tacttgccgc agacctggaa taatcagcaa aggcaaaatt cacaaggtaa gggatgatct
    21781 gtaaccggat ttcttcactc aatttgttca atgtcgggtt atccagtgcc atcgcgtaat
    21841 tgagcagacc ttcaatcgca aactgaggat cctgaacgcc aacttttgcc aggaataagg
    21901 taaacccaag cggtgctttc gactgcacca tcccctgtgc agccatcaca tccgtaatcg
    21961 ttaaaccacc tgagcctgtc gccggtgcgt catcactcaa ttttggagat tttggggagt
    22021 aatattttgg taaggcttca aggttcatgc tcgttctcca cttacgccag tacgcctatt
    22081 gccagcgcac gatcgataaa acgaaatatc agctccaact gggagccata catctcttca
    22141 aatgccacgg tatccgcatg cagctcgtcg tgatgctttc tgcacaaagg caacacaaag
    22201 aggtcatgcg cttttgtacc cattcccccc tgaccgtggc ctatcaggtg gtggggatca
    22261 tcagcaagct ttccacaaca tgcacacggc tgtgtcttaa cccagcgcgt gtacttttca
    22321 ttaacccagc ggcgacgttt tgggcgtaac ataaaagact ccggcgactc cggatccact
    22381 ttcagcgcca gcaccttttt cgctttatcc tggataatgc tggtggcagg aaccgaaggc
    22441 acaaggtcac tttcccgggt gacagacggc acaacaggct tcggtaatct cagtgcctta
    22501 cgggctgcac tttccggtaa ggcatccgcc aggtcattac gaatcagcca ccagcacagt
    22561 tccggcattg tcacaacgtg actatcatca aaaccgagat cccgacgcac aacagacaac
    22621 acccaacggg cacagttatc cgttgccatt gattccagcc gttccgtgaa ctgatcgcgc
    22681 agctggttat cgcagtgcca gcagagacgg attgcgcccg gagcgtgccg cattgtggtc
    22741 atgttctcgc tgtgccagtc ggaatgaggc cactggcagc ctttttcacg aagtaaccag
    22801 ctttcaagac attccacccc accagcacga cggatcactg cctcattgcg gaacacggcc
    22861 cgaacggcag gatcatccgc cagcggttgt gatgccgcgg gaacggcacc actggcgaaa
    22921 gatgaataac gttccggctc aggctccagc aggacacgcc cctgcataaa caggggcatc
    22981 agctctgaac cgggcctgaa caatacgatc cccatacgcg gggcaatttc aggggtcagt
    23041 agtgctctca cagtcacctc aatgaacggt atcgagcaac tttaacagct cggggaatcg
    23101 ggattcgaag aaatgcggct gcgtctcgcg cggatttgcg ggactggtga tgttcttgcc
    23161 gaacatgcag cctttcgccg tcagcgacca gaattttttg atgttgttaa tcgcggtacg
    23221 gctgtatcgt tcgcgttgtt caacgattcc cagcttcacc atctggtgat atgcctgatt
    23281 agccgtcagg cggataccat actgtttcag cagtgcactc agcgacagcg tggggcgact
    23341 tgagccatca ggcgcgtcag caggagcatc aatggcatag cgcggtgcca gattcggtaa
    23401 gccaacagcc tcctggagtt tctgacaggc accaagcact gatgagttag acaggtttaa
    23461 ttcccggcgc ataaagtcca gcaggatcac accagcctgc atcttgtcag cagcctgccc
    23521 agataatttt tccggtgcgc tggttaccat atcgaaagta cggatcacct tcagatggaa
    23581 tgacgagctg atccacattg cataggcata caccagttct ttgcagacat acgtcccctg
    23641 gttatttccg ccattaatga cgctaactgg ttgattttgt tccataggcg gaattccacc
    23701 ctcggtgaaa agttgttcaa tcaattcaca ggtttgctta ttggagagcc agtatttcgg
    23761 gcggtttttt tgttctcccc cggctgccct gtgcagatcg ttcaggctgt aacgcccata
    23821 agcatcacga cgaacttcaa taccatcaat gaccatcaga ttattcatac ttcgtttctc
    23881 ctcttgatca ggcggctgca cccgccgttt tctcgtactt actgatagtg atctcgacct
    23941 tcccttccgg gataaccggt ccccactcca ccagcattct tttcacctga ctgtcgtctt
    24001 cccacacacc cgcgtgggtc agggcgtcaa acagcgcctt gttatagttg tccagatcgc
    24061 ggatccggtt atccggagga aacaacacga tctccactga agcaggtgcc gacgttggtt
    24121 tcggaagacg acgtaactgc tcaactattg ctgcgcacgc cgcgctctgg aatttgcgcc
    24181 ccgccgcgct tatcaggctt ttaccagcaa acgccccttt gttagggtgt cgccagtacg
    24241 tattcacgct gggcgggaaa ggcaggatca gtttcatact ttcaggcctc tctcatgtaa
    24301 ccagtgggtt gcacgcagcc tggcgttttc ctcaccggca agcagtgagc ggataatcca
    24361 gacagcctcg ctgtcgtcgt ccttcaccgc ggtatgaagc gttatccccc gggccacgcc
    24421 acgctttatc gtgatgacgc cttttttctc cagtgcgcga agatgctcca ccgctgcatt
    24481 cactgaacgg tatcccagca tggttgccac ctcctgattg gttggcggga agcctcgttc
    24541 tttctggtaa gaaatcagca tatccagcac ctgctgctgg cattgagtta atgtcgtcat
    24601 gccaccatct ccctgaccag tttttccgcc tgctggcgaa cctgcgccag aaaggcctca
    24661 ccacatgcct caagttcatc gcgcccgatg tagctgattg ccggtccctt ccaggtcttg
    24721 tcaaaaacag caatagcacc agagaaaaaa gctcctgtcg gtatctgctt ctcgtctttc
    24781 gggataaacc agacaggcag ttcaaaacca atacgcccgc gaataaaagc aatatgatct
    24841 gcatcttccg gccaccacac ttcgctggtg gcagctttga tcaggaaaac atagcgcccg
    24901 cccttatcac gcatggcact ggcatgtttc atgatgtaac gcatgccggt gatgtattgc
    24961 ccctcatgct gactggcgcg gctgtatgga ggattaccaa aggcagcacc tttaagctcc
    25021 gcaaggcgtt ctgaccagtc atgcgccagc gcgttgtctt ccgccgtgta atacgcggca
    25081 catttggcgt tatcaccgtc agtgaacaga tccagaacaa acgggccaaa cagggtgtta
    25141 attccccaga aaatgttgtc cggcgtgcgc cactgatcac ccacttcctt cagttcatgg
    25201 gctggtttgt tccgcagttc caccagctcc tggcaatatt tattactcat taagccccca
    25261 cgtaattccc tgacagatac cactcatcac ccgatacagc gcgcttgctg cttttccgta
    25321 aacactgctc acgacgcgcc agaaaattgt ttcgttctgg ctgggagtgg ctttcacgga
    25381 atgccgccat ccacaccgtt gcagcacgac ggtataagcc cctggactcc agttcttccg
    25441 cctggcgggt caggcacaaa atcacccggg gatcgttagt gccgacatag aaattgcgca
    25501 caggtctggt ttcacgaact ggttgtggtt ccgactcctg cgctctctca gtcaggcgcg
    25561 ggaaatgtct gcgtgtatct ccttcacaac ggtgagccac acgcccactc tgacgtaact
    25621 tgcttgctga ctgcagaacg cgctgccgtg agtaacctgc aaaagcatcc gcaatgtctc
    25681 cggaagtaca ccccggatgg gcttcaatga atttctgaac atcattcaaa agactcataa
    25741 tcaccccctg aatcctgctg ggatctggct gtagtccacg ttgtcgtaac tggctttgaa
    25801 gtacgggtct tcgcgttttt cggtgtacgt gctgacggac ggcgataagc gcagggaaag
    25861 ctcatcccat ttttcccgca gcttcgacgg gctgagcacg ttacggcacc agaacggatc
    25921 gcgactgacg cggctgtaca tctcgcagat ttgtttatga gtacgaccat cctgcacaca
    25981 catcaggcga atttcgtttg cccaggctgt ccagttcggt tctttgggac gaaccacctc
    26041 gccgtcacat tcggcggcat gctcgtacag ggcgatgatt tttttccaga gccactgtgc
    26101 gcaggtcaaa tcatcctgcg tcccccactg gcgcttttta gggctgaata caaccgcatc
    26161 aggatggcga gttaaaaaat cctgttcatc cgtctgcgtg tccggttgcg aagcgtccgg
    26221 acgagaaggt tttttatctg acggatcatg ttttgatttt actgacggat ccccgccaga
    26281 ttctgacggg tgaaaacccg cttttttgcc agatttcgac gcatcaaatt ttgacgggtc
    26341 agattttgat gcgtcagatt ttgacgggtc agaatctgac agttgagaaa atgccgctgc
    26401 ctgaagcttc gcaacgttaa gctgataaac attcgacgca ttgcggttac cctggcgacg
    26461 cgccttacgc gttaaccagc cttctgcttc cagccgtgcg atagccgttc tgacggtact
    26521 catccccgcg ccaatctgac gggcaatggt ttcaattgat ggccagcaca caccttcgtc
    26581 attactgaaa tcagccaggc gggccataat tgccacgctg gataacttca tgcctgatgc
    26641 agcgcaacca tcccatacat agccggttaa tttagtgctc atgaccgacc tctatttccc
    26701 tgaatttacg acgaaactgt tcgagcggac tgaagcattc atgctcataa ccttcgcgga
    26761 ggtagataac ccgttgtgtt tccggctccc aacgaatgac tctgacgagc actccgtagt
    26821 gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc ggttgaaatc
    26881 acccacagcc cactctgcaa agctgtgggt tacaatttcc ctgtcacctg gtacattcac
    26941 tgcatagcaa tattccacct tcgcttttcc acccggtaca ggaagcgcaa tcagttgcga
    27001 gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc agaagcaatc
    27061 gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga aatgcaaaaa
    27121 attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata cagctcgtcg
    27181 ttaattgctg tcttctcatg cggttccact acgccgtctt cgattgctga acgaatctgt
    27241 tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat atcggcgttg
    27301 tccacatcct cgacgtcagg aagagacaca aagacgccat ttgcagactg cgccacagca
    27361 tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc cagcgggaaa
    27421 atctgatcgc catctgcacg aaggcggttg aataaagcgt tctctgttac atccagccag
    27481 tcagcggctt cagcgtaacc ccccggcaac gccgcgatag tttttctgac agctttcacg
    27541 taccactcag gctgtttttc tattttccag tgatgcttac ccacggctat ctccttaaaa
    27601 ctgtggttac ttttcatctg atgaatcttt aatcttttga aaaatatctg gacgtaattt
    27661 ttcttttgat atgccagtgg tcttttcaat gaatatcgag agctttgcag ggggacgctt
    27721 ttctctgttc aaccagttcc agacatgttg ttgctttact aaatgaccgc tgctggctgt
    27781 gagcttccga gccaattctg attgaccacc agccagagcg attgcctccg ataaggctaa
    27841 ttgctcaggt gtcatagctt tctccttttt tggtagttaa gttgttacga gctgcaagaa
    27901 tacaacatta acaactttca ccacaacttt taggtgttgg aaagctaaaa cataaagttg
    27961 taacctcatc aaaaaagaga gggatatgtt gtgaaaacac tggcagaacg attaaagata
    28021 ggtagagaga aagctggcat gagccaagct caactagctg aaaaaattgg actttcacaa
    28081 caatctgtag ccaaaataga gaatggcgaa actctacaac cgcgcaaaat taaagaaatt
    28141 gcaaaagttt taggtgtatc acaaaagtgg ttacaacttg gtattgaaga caacgcatcc
    28201 atacctgatc ttgttgtaaa agaagcagaa agcaccgcat tagaccccga tattttcgta
    28261 aacattcctg ttttagatgt cgagttatcg gcaggtaacg gatgtctggc tgaaatagtt
    28321 gaatcagcta ttgactggtt tccgttaaga agagcagatt tgagaaaatc tggcgtatgt
    28381 gcatctaatg ccaagatcgt acaaatatgg gggaacagtt tattaccggt tctcaataat
    28441 ggagatcttg ttgccgttga tatttctcaa accgttccta ttcgtgatgg cgatctttat
    28501 gccgtacgag atggtgtatt gctaagggtt aaaatactta tcaacttacc tgacggtggc
    28561 ttgattctta gaagcttcaa caaagatgag tacccagatg aaatactcac ctttgaagat
    28621 agacgagcca gaattcatgt tataggtagg gtattctggt catcgcgaac ttggtaatgc
    28681 atcgaaaagc atttcttcag aaataatttt aagttttgca ccattatcat ccctataaga
    28741 tatagccttt tcgatcttcc ttccgtgact agagaatttc caatcacggg aggaaagcgt
    28801 cccaattact aaaaaatcca acttttgagt aattccacta ctgatgttcc caccagcatt
    28861 tttaatcaaa ttttcaacta cggctctctt tcctgcaaca aaagtgcctg taagacaata
    28921 ggttttaccc tctaactcta tcgaagcccc tacatcaata ggcagcctgg tcgccaaacc
    28981 atccaccacc ccactttcca agtcacatcc tgtgaagtct actaatgcct tatgtagagt
    29041 taaactctca tcttcagtaa taacaccatc tttaagaatt tcctttacaa gtgcataaag
    29101 tttttttcct gggtagttgt tcttcaaagc tccattttgc tcaagccacc aattaagata
    29161 tcttatttct tcttgagtta agttccgatc agcaattaat cctttacata gtccattaag
    29221 taaatggaca tctacatcct tggagtaaaa atcaatttca gggatatcaa gaatttccct
    29281 ctgtatttgg agaaggctat ttttaaggtc atcacgttct tctgatgtga ttattccatc
    29341 cgcaagaata tccgacaccc gagcagatag actttttata actccattat tgataatctg
    29401 ctttgcttca agtaaccatg tatctaagta aagaacctcc tcttcacgga caactccatc
    29461 tgcaatgatt ccatcaatga tgctaatcaa gttagcaaat aacttgtccc ggttctgtgt
    29521 gtaattaaaa gcgtaaagcg cgtcttccat acaacctcct ttttttgata atccttgcac
    29581 tccttggcta ctcgttcaaa ccacataaag ttgttgacaa cattcaaaac cacaactaaa
    29641 ttacaactta aaggtgttaa gacaacgaac aggcaggaca cccacgaagt agcccgcccg
    29701 gtacgtacga agaccgggat gattcgttag cggatgattt cagtggagag aatagatgaa
    29761 tgagcaggat ttgaagcatg tgatcgcatt gttgctggaa gacgctaaac gtttgcagca
    29821 gatagagcca aatgcaggca ctgaggcccg tattttgtta gcaaaacagg cattaaagac
    29881 ttgcggggcg caagatcctg atcgaaccaa gttcatgaat ttcatggcta acacgatcac
    29941 ccccctgcca tgcaatggag agagggtgag ccgtgtttat cacgacacaa tggttaaggc
    30001 attaagaatc gagcttgatg ggcttaggcg taagatcgtg atgaacaaaa tcgttgccaa
    30061 ctaaggaagc agacggaagt aagcatgcgc tttgttcaaa tttgcagaca aatatatttg
    30121 cgtcaacacc agcactgtta gcaatggaaa aagtttgatc aaggatttgt tggcagttca
    30181 ttgtgctttt gaggatatat ccctctggaa tcagtctgca gcagctatcg tcagactctt
    30241 tgattgtttt ctggtacaga aagttaagca ttaattcttc aaattttttg gtctgttcgg
    30301 ctgttgcttc aaacagacga acgtgaacat aaaactggtt cattaggttt ccttgctggc
    30361 tgtgtgagaa ctccagcata ccaccgagcc tgaagtggtg aaaagacagg caatagtttc
    30421 attgctgtgt gtagtcttgg cggtaccagc ttgtaccctt gcttccggct ggtaccgtcc
    30481 tttttacaaa acagagaaga gcatcaccgg acgacgggct cataacccaa tccatccggg
    30541 cggcggccac cacaggtgtt cttctctgtt ttgtggagaa actaataggc cttgcagggt
    30601 cgatatgatg aggagcagca aaatggctag cgaacgcagt actgatgtac aggcatttat
    30661 cggggagctg gacggcggcg tatttgaaac caaaatcggc gcagttctca gtgaagtcgc
    30721 ttccggtgtg atgaacacga aaaccaaagg taaggtctcg ctcaacctgg aaattgaacc
    30781 atttgatgag aaccgtgtga aaatcaaaca caaactctca tatgttcgcc caactaaccg
    30841 cgggaaaatt tccgaagaag acactaccga aacgccgatg tatgtcaatc gcggtggtcg
    30901 cctgactatt ctgcaggaag accagggaca attactgact cttgccggtg aacctgacgg
    30961 aaaactccgc gcagcaggtc gttaatatcg ttcttaataa actgattatt tatctcatca
    31021 ctgaatatct ttgtatagtg aggacttatt atgtctcaga acttagacgc aaccgcaatt
    31081 aatcaaatcc atgcccttat ttctgctcag ggtgttaatg aaattatcag taagattggt
    31141 gccgacgcag tggcattacc tgaaaatttc cgcattcatg atctggaaaa atttaattta
    31201 aatcgcttcc gtttccgtgg tgcgctttcc actgccagca tcgatgactt tacccgttat
    31261 tctaaagatc ttgcagatga aggcacccgc tgctttatcg atgctgataa tatgcgtgcc
    31321 gtcagtgtgc ttaacctggg tactattgat gaaccaggtc acgcagataa caccgccact
    31381 ctcaaactga aaaaggcagc gccgttctct gctctgttgt ctgttaatgg cgagcgtaac
    31441 tcccagaagt cactggcaga atggattgaa gactgggccg actaccttgt gggctttgat
    31501 gctaatggtg acgctattca ggcaacaaaa gcggctgcgg ctgtccgtaa aatcacgatt
    31561 gaagcaaacc agaccgctga ttttgaagat aatgacttca gcggcaaacg ctccctgatg
    31621 gagtctgtcg aagcgaagac caaagatatt atgccagtgg catttgaatt taaatgcgtt
    31681 ccgtttgaag gtctgaaaga acgtccgttt aaattacgcc tcagcattat cactggcgat
    31741 cgtcctgtac tggttctgcg cattattcag ctggaagcag tgcaggaaga aatggctaac
    31801 gaatttcgtg atctgcttg
//
LOCUS       DAZFFI010000048        31785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFFI010000048 DAZFFI010000000
VERSION     DAZFFI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.048"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(214..1587)
                     /gene="argH"
                     /locus_tag="ACHJ12_002575"
     CDS             complement(214..1587)
                     /gene="argH"
                     /locus_tag="ACHJ12_002575"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4990345.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1648..2424)
                     /gene="argB"
                     /locus_tag="ACHJ12_002576"
     CDS             complement(1648..2424)
                     /gene="argB"
                     /locus_tag="ACHJ12_002576"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4990346.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2432..3436)
                     /gene="argC"
                     /locus_tag="ACHJ12_002577"
     CDS             complement(2432..3436)
                     /gene="argC"
                     /locus_tag="ACHJ12_002577"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4990347.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3590..4741
                     /gene="argE"
                     /locus_tag="ACHJ12_002578"
     CDS             3590..4741
                     /gene="argE"
                     /locus_tag="ACHJ12_002578"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4990348.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5093..7744
                     /gene="ppc"
                     /locus_tag="ACHJ12_002579"
     CDS             5093..7744
                     /gene="ppc"
                     /locus_tag="ACHJ12_002579"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4990349.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7927..9660
                     /gene="eptC"
                     /locus_tag="ACHJ12_002580"
     CDS             7927..9660
                     /gene="eptC"
                     /locus_tag="ACHJ12_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4990350.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9875..10726
                     /gene="yijO"
                     /locus_tag="ACHJ12_002581"
     CDS             9875..10726
                     /gene="yijO"
                     /locus_tag="ACHJ12_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4990351.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10713..11054)
                     /gene="frwD"
                     /locus_tag="ACHJ12_002582"
     CDS             complement(10713..11054)
                     /gene="frwD"
                     /locus_tag="ACHJ12_002582"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4990352.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11056..11930)
                     /gene="pflC"
                     /locus_tag="ACHJ12_002583"
                     /pseudo
     CDS             complement(11056..11930)
                     /gene="pflC"
                     /locus_tag="ACHJ12_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
     gene            complement(11896..14193)
                     /gene="pflD"
                     /locus_tag="ACHJ12_002584"
     CDS             complement(11896..14193)
                     /gene="pflD"
                     /locus_tag="ACHJ12_002584"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4990353.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDDVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14244..14564)
                     /gene="frwB"
                     /locus_tag="ACHJ12_002585"
     CDS             complement(14244..14564)
                     /gene="frwB"
                     /locus_tag="ACHJ12_002585"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4990354.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14579..15658)
                     /gene="frwC"
                     /locus_tag="ACHJ12_002586"
     CDS             complement(14579..15658)
                     /gene="frwC"
                     /locus_tag="ACHJ12_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4990355.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15967..18468
                     /gene="ptsP"
                     /locus_tag="ACHJ12_002587"
     CDS             15967..18468
                     /gene="ptsP"
                     /locus_tag="ACHJ12_002587"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4990356.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKSVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18480..19142
                     /gene="fsa"
                     /locus_tag="ACHJ12_002588"
     CDS             18480..19142
                     /gene="fsa"
                     /locus_tag="ACHJ12_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4990357.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19153..20256
                     /gene="gldA"
                     /locus_tag="ACHJ12_002589"
     CDS             19153..20256
                     /gene="gldA"
                     /locus_tag="ACHJ12_002589"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4990358.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20531..21148
                     /gene="yijF"
                     /locus_tag="ACHJ12_002590"
     CDS             20531..21148
                     /gene="yijF"
                     /locus_tag="ACHJ12_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4990359.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21175..22080)
                     /gene="yijE"
                     /locus_tag="ACHJ12_002591"
     CDS             complement(21175..22080)
                     /gene="yijE"
                     /locus_tag="ACHJ12_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4990360.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22174..24354)
                     /gene="katG"
                     /locus_tag="ACHJ12_002592"
     CDS             complement(22174..24354)
                     /gene="katG"
                     /locus_tag="ACHJ12_002592"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4990361.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24683..25573)
                     /gene="metF"
                     /locus_tag="ACHJ12_002593"
     CDS             complement(24683..25573)
                     /gene="metF"
                     /locus_tag="ACHJ12_002593"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4990362.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25649..25783
                     /locus_tag="ACHJ12_002594"
     CDS             25649..25783
                     /locus_tag="ACHJ12_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990363.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25922..28354)
                     /gene="metL"
                     /locus_tag="ACHJ12_002595"
     CDS             complement(25922..28354)
                     /gene="metL"
                     /locus_tag="ACHJ12_002595"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4990364.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28357..29517)
                     /gene="metB"
                     /locus_tag="ACHJ12_002596"
     CDS             complement(28357..29517)
                     /gene="metB"
                     /locus_tag="ACHJ12_002596"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4990365.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29794..30111
                     /gene="metJ"
                     /locus_tag="ACHJ12_002597"
     CDS             29794..30111
                     /gene="metJ"
                     /locus_tag="ACHJ12_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4990366.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30171..30779
                     /gene="yiiX"
                     /locus_tag="ACHJ12_002598"
     CDS             30171..30779
                     /gene="yiiX"
                     /locus_tag="ACHJ12_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4990367.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30924..31599
                     /locus_tag="ACHJ12_002599"
                     /pseudo
     CDS             30924..31599
                     /locus_tag="ACHJ12_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(31686..>31785)
                     /locus_tag="ACHJ12_002600"
     CDS             complement(31686..>31785)
                     /locus_tag="ACHJ12_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO4990368.1"
                     /translation="HNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
BASE COUNT         7656 a         8859 c         8081 g         7189 t
ORIGIN      
        1 aatatgattt tcatggggag tgtgaaggta aagaagacac aatgataaaa aggaatgtga
       61 caaataatgg ttctggttgg gcgttaaatt taagctcacc agaatgctga caaatttctg
      121 cctgatgtgg cgtaaacgcc ttatccgcct acgaagtgtc gcactttgta ggcgtggtaa
      181 gctccccatt ttcaggggag caatataaac aaattactct aaccgcgcct gcgcaaaagc
      241 aattgcctgc gccacctgct gcggtgagac gccgcctttt gccgcacgct tgtcgaggca
      301 cgattgcagc gacagaatcg gatagacatc ttcgtcaatc accgggctga atttctgcaa
      361 ctcgtcgagc ggcagatctt ccagcggttt gccctgacga atggcttcca ccaccgcttc
      421 accaacaata tggtgcgcct cgcggaacgg tacgcctttc gccaccagat aatccgccag
      481 ttcggtggcg ttggcgtaac cctgctgagc cgcttcctgg caacgtggac gtttcacctg
      541 aatgccgtcc agcaccagcg ccgccatatg caggcagtcc agccaggtat cgagcgcgtc
      601 gaacagacct tctttgtctt cctgcatatc tttgttgtaa gccagcggca aacctttcag
      661 cgtcatcatc atgccggtta acgccccctg cacccgaccg catttaccgc gaatcagctc
      721 cagcgcatcc gggtttttct tctgcggcat taatgatgaa ccggaagtca cgcggtcaga
      781 aagctccaca aaccccgctt cgccggtgtt aaagaaaatc agatcttcag caaaacgcga
      841 cagatgcacc atgccgatag cggcagcaga aagcagttcc aacacatggt cacggtcaga
      901 aacgctgtcg agactgttac gggtcgccga agcaaagccc agccagcctg ctaactgttc
      961 acggtcgatt tcataggccg ttcccgccag cgcgccacag cctagcgggc tgacatccag
     1021 acgcttaagc gcatcctgca aacggctttc atcacgcgcc agcatctcaa cataggccag
     1081 gcaccagtgc gcgaacgtca ccggctgggc gcgttgcagg tgagtgtaac ctggcattac
     1141 cgcgtcctga ttgttttgtg cggtttccac cagcgccgat tgcagctgcc ggttagccgt
     1201 cagtaactcg ctaacggtat ctttgcacca cagtttcagg tcagtcgcta cctgatcatt
     1261 acggctacgc ccggtatgca gctttttgcc taactggccc actttgtcga tcagtttgcc
     1321 ttccacccag ctatggatat cttcggcgtc gctttcaagg atttgttgtg gcctggcgcg
     1381 aacatcttcc agcaacacgt tcagcgcctc ttccagttgc gcctgctctt ctgcggttaa
     1441 cacgcctacc gtgaccaggg ctttggacca ggccacagag ccaacaatat cctgctccgc
     1501 cagacggtaa tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat ctgctgcctg
     1561 ggtaaaacgc ccgccccaaa gtgccataac tctgtttcct tattttgaaa ttcaatgccg
     1621 gaaagctgcg cctccggcca acaaaactta agctaaaatc cgcgtaccca tcggcatacc
     1681 gttaaacagt gccggaagct gctccgcatg acgccaggag gcgatatcta ccggacggcc
     1741 cagcgtgcgg gccgcatcca gcgccgcgtt cactttcact atcatgccgt cagtaataat
     1801 gccctgctca atcagttgtt ctgctttcgc ggcggtcatt tcggcaatgc gttgcccttt
     1861 gccgtcgaga atgccgctga cgtcggagag caaaatcaga tccgcgccca gcgttgccgc
     1921 cagcgccgtt gccgcctggt cggcattgac gttcatcagt tgcccttcgt ctgttacgcc
     1981 aatggagctg accaccggca gataaccgtt ctccagcaag gagttgataa gcttaggcga
     2041 acctggctgc gccagtccaa catgacctaa ctcttcatca agctgggtca ctttgacgct
     2101 gtcgccgtca ccgagaaaca aacctacggc cgcaatctga tgtttcttcg cccatgccaa
     2161 cagggtttta tttgccgttc ccgccagtgc tccggtgata atgtctatct gatcagcagg
     2221 cgtcacccgc aggccgtttt tctttttcac cggcagattc agccctttca tcagctcatc
     2281 caccacgcaa ccgccgccgt gcacaatcac cagcggacgc tgatgtgact cacgataatt
     2341 caccagtgcg ctaaacagac gttccagcgc ctcttcacta tccagcagta cgccgcccag
     2401 tttgataatt aatggattca tcattgcacc cttaaataag agactgcgtt tccgcatagc
     2461 cgaaacgaat attggcgcac tgtaccgctt gtgccgccgc gcctttcagt aagttgtctt
     2521 cggtcgccac aataatcaga tgctcgccct gaacggcaaa cccgatatcg caaaatggca
     2581 gcccaacgac atttttcagc gccggaacgc ctttgtcata cagccgcacc agcggtttat
     2641 gggcatacgc ctgctgtaac acttgcgcga cttgcgcctg ggtcacaccc gatttcaggc
     2701 ggcaggtaat ggtttcgaga atgccgcgcg ggaaattgcc cagatgtggg gtgaagataa
     2761 cgtcagcacc gaggtgtgtg gcgatctctg gttgatggcg atgagtaaag acgccatacg
     2821 gttgcaggct aacttcacaa aagctgtttg aaatggccgc tttacgccct gcaccgctca
     2881 cgccgctggt ggcgttgatc accggccact gattgaggtc aagaagatcg gcatcaatca
     2941 acggtttcag cgccagctgt gccgccgtcg gataacagcc cggcaccgca atcaaattcg
     3001 cttcttttaa tttattaccg caccactccg ccagaccgta ggctgcctgt tccaacagtt
     3061 ccgggtattg atgggtaaag ccgtaatatt tttcatagaa ggtggcgtcg ttaacacgaa
     3121 acgcgccgga aaggtcgaac accacgcagc ccgcttcaag aaactgcggc gctaaatcgt
     3181 ggctaacttc atgggcggtg gcgagaaaca ctacgtccac ccctgggcta aactcgctga
     3241 tatccgacat cggctgcaac ggcagatcaa cgatgccttt tagctgcgga tgcaaatcgg
     3301 agattaactt tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt atgttcatat
     3361 gcggatggcg atttacatag gtcactagct ctgcgccagc gtagccgctg gcacccacaa
     3421 tcagcgtatt caacatcggg gctattcacc ttcttatgtc tggttgccag gttaaacgta
     3481 aaacattcac cttacggctg gtgggtttta ttacgctcaa cgttagtgta tttttattca
     3541 taaatactgc atgaatattg atactatcat gaccagaggt gtgtcaacaa tgaaaaacaa
     3601 attaccgcca tttatcgaga tttaccgcgc tctgattgcc acaccttcaa taagcgccac
     3661 ggaagaggca ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg actggtttaa
     3721 agatttgggc ttcaatgtgg aagtgcagcc tgttccagga actcgcaaca aatttaatat
     3781 gctggccagt tgcggacagg gggctggcgg cttgttgctg gcggggcata ccgatacggt
     3841 gccatttgat gacggtcgct ggacgcgcga tccgtttaca ctgacggagc atgacggcaa
     3901 gctttacggc ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc ttgatgcgct
     3961 acgcgatgtc gacgtcacga aactgaaaaa accgctctac attctggcga ctgctgatga
     4021 agaaaccagt atggccggag cgcgttattt tgccgaaact accgccctgc gcccggattg
     4081 cgccatcatt ggcgaaccga cgtcactaca accggtacgc gcacataaag gtcatatctc
     4141 taacgccatc cgtattcagg gccagtcggg gcactccagc gatccagcac gcggagttaa
     4201 cgctatcgaa ctaatgcacg acgccatcgg gcatattttg caattgcgcg ataacctgaa
     4261 agaacgttat cactacgaag cgtttaccgt gccataccct acgctcaacc tcgggcatat
     4321 tcacggtggc gacgcttcta accgtatttg cgcttgctgt gagttgcata tggatattcg
     4381 tccgctgcct ggcatgacac tcaatgaact taatggtttg ctcaacgatg cattggctcc
     4441 ggtgagcgaa cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc cgatccctgg
     4501 ctatgaatgc ccaccgaatc atcaactggt tgaagtagtt gagaaattgc tcggagcaaa
     4561 gaccgaagtg gtgaactact gtaccgaagc gccgtttatt caaacgttat gcccgacgct
     4621 ggtgttgggg cctggctcaa ttaatcaggc tcatcaacct gatgaatatc tggaaacacg
     4681 gtttatcaag cccacccgcg aactgataac ccaggtaatt caccattttt gctggcatta
     4741 aaacgtaggc cggataaggc gctcgcgccg catccggcac tgttgtcaaa ctccagtgcc
     4801 gcaataatgt cggatgcgat acttgcgcat cttatccgac ctacaccttt ggtgttactt
     4861 ggggcgattt tttaacattt ccataagtta cgcttattta aagcgtcgtg aatttaatga
     4921 cgtaaattcc tgctatttat tcgtttgctg aagcgatttc gcagcatttg acgtcaccgc
     4981 ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc gagcatattc gcgccaatgc
     5041 gacgtgaagg atacagggct atcaaacgat aagatggggt gtctggggta atatgaacga
     5101 acaatattcc gcattgcgta gtaatgtcag tatgctcggc aaagtgctgg gagaaaccat
     5161 caaggatgcg ttgggagaac acattcttga acgcgtagaa actatccgta agttgtccaa
     5221 atcttcacgc gctggcaatg atgctaaccg ccaggagtta ctcaccacct tacaaaattt
     5281 gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt cagttcctga acctggccaa
     5341 caccgccgag caataccaca gcatttcgcc gaaaggcgaa gctgccagca acccggaagt
     5401 gatcgcccgc accctgcgta aactgaaaaa ccagccggaa ctgagcgaag acaccatcaa
     5461 aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg gctcacccta ccgaaattac
     5521 ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc tgcttaaaac agctcgataa
     5581 caaagatatc gctgactacg aacacaacca gctgatgcgt cgcctgcgcc agttaatcgc
     5641 ccagtcatgg cataccgatg aaatccgtaa gctgcgtcca agcccggtag atgaagccaa
     5701 atggggcttt gccgtagtgg aaaacagcct gtggcaaggc gtaccaaatt acctgcgcga
     5761 actgaacgaa caactggaag agaacctcgg ctacaaactg cccgtcgaat ttgttccggt
     5821 ccgttttact tcgtggatgg gcggcgaccg cgacggcaac ccgaacgtca ctgccgatat
     5881 cacccgccac gtcctgctac tcagccgctg gaaagccacc gatttgttcc tgaaagatat
     5941 tcaggtgctg gtttctgaac tgtcgatggt tgaagcgacc cctgaactgc tggcgctggt
     6001 tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg aaaaacctgc gttctcgcct
     6061 gatggcgaca caggcatggc tggaagcgcg cctgaaaggc gaagaactgc caaaaccaga
     6121 agggctgctg acacaaaacg aagaactgtg ggaaccgctc tacgcttgct accagtcact
     6181 tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg ctcgacaccc tgcgccgcgt
     6241 gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt caggagagca cccgccatac
     6301 cgaagcgctg ggcgagctga cccgctacct cggtatcggc gactacgaaa gctggtcaga
     6361 ggccgacaaa caggcgttcc tgatccgcga actgaactcc aaacgtccgc ttctgccgcg
     6421 caactggcaa ccaagcgccg aaacgcgcga agtgctcgat acctgccagg tgattgccga
     6481 agcgccgcaa ggctccattg ccgcctacgt gatctcgatg gcgaaaacgc cgtccgacgt
     6541 actggctgtc cacctgctgc tgaaagaagc gggtatcggg tttgcgatgc cggttgctcc
     6601 gctgtttgaa accctcgatg atctgaacaa cgccaacgat gtcatgaccc agctgctcaa
     6661 tattgactgg tatcgtggcc tgattcaggg caaacagatg gtgatgattg gctattccga
     6721 ctcagcaaaa gatgcgggag tgatggcagc ttcctgggcg caatatcagg cacaggatgc
     6781 attaatcaaa acctgcgaaa aagcgggtat tgagctgacg ttgttccacg gtcgcggcgg
     6841 ttccattggt cgcggcggcg cacctgctca tgcggcgctg ctgtcacaac cgccaggaag
     6901 cctgaaaggt ggcctgcgcg tgactgaaca gggcgagatg atccgcttta aatatggtct
     6961 gccagaaatc accgtcagca gcctgtcgct gtacaccggg gcgattctgg aagccaacct
     7021 gctgccacca cctgagccga aagagagctg gcgtcgcatt atggatgaac tgtcagtcat
     7081 ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa gattttgtgc cttacttccg
     7141 ctccgctacg ccggaacaag aattgggcaa actgccgttg ggttcacgtc cggcgaaacg
     7201 tcgcccaacc ggcggcgtcg agtcgctgcg cgccataccg tggatttttg cctggacgca
     7261 aaaccgcctg atgctccccg cctggctggg tgcaggtacg gcgctgcaaa aagtggtcga
     7321 agatggcaaa cagagcgagc tggaagccat gtgccgcgat tggccattct tctcgacgcg
     7381 tctcggcatg ctggagatgg tcttcgccaa agcagacctg tggctggcgg aatactatga
     7441 tcaacgtctg gtagacaaag cactgtggcc gttaggtaaa gagttacgca acctgcaaga
     7501 agaagacatc aaagtggtgc tggcgattgc caacgattcc catctgatgg ccgatctgcc
     7561 gtggattgca gagtctattc agctacggaa tatttacacc gacccgctga acgtattgca
     7621 ggccgagttg ctgcaccgct cccgccaggc agaaaaagaa ggccaggaac cggatcctcg
     7681 cgtcgaacaa gcgttaatgg tcactattgc cgggattgcg gcaggtatgc gtaataccgg
     7741 ctaatcttcc tcttctgcaa accctcgtgc ttttgcgcga gggttttctg aaatacttct
     7801 gttctaacac cctcgttttc aatatatttc tgtctgcatt ttattcaaat tctgaatata
     7861 ccttcagata tccttaagga attgtcgtta cattcggcga tatttttttc aagacaggtt
     7921 cttactatgc attccacaga agtccaggct aaaccccttt ttagctggaa agccctgggt
     7981 tgggcactgc tctacttttg gtttttctct actctgctac aggccattat ttacatcagt
     8041 ggttatagtg gcaccaacgg cattcgcgac tcgctgttat tcagctcgct gtggttgatc
     8101 ccggtattcc tctttccgaa gcggattaaa attattgccg cagtaatcgg cgtggtgcta
     8161 tgggcggcct ctctggcggc gctatgctac tacgtcatct acgggcagga gttctcgcag
     8221 agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag ccagcgagta tttaagccag
     8281 tatttcagcc tgaaaattgt gcttatcgcg ctggcctata cggcaatggc agttctgctg
     8341 tggacacgtc tgcgcccggt ctatattcca aagccgtggc gttatattgt ctcttttgcc
     8401 ctgctttatg gcttgattct gcatccgatc gccatgaata cgtttatcaa aaacaagccg
     8461 tttgagaaaa cgttggataa cctggcctcg cgtatggagc ctgccgcacc gtggcaattc
     8521 ctgaccggct attatcagta tcgtcagcaa ctaaactcgc taacaaagct actgaatgaa
     8581 aataatgcct tgccgccgct ggctaatttc aaagatgaat cgggtaacga accgcgcact
     8641 ttagtgctgg tgattggcga gtcgacccag cgcggacgca tgagtctgta cggttatccg
     8701 cgtgaaacca cgccggagct ggatgcgctg cataaaaccg atccgaatct gaccgtgttt
     8761 aataacgtag ttacgtctcg tccgtacacc attgaaatcc tgcaacaggc gctgaccttt
     8821 gccaatgaaa agaacccgga tctgtatctg acgcagccgt cgctgatgaa catgatgaaa
     8881 caggcgggtt ataaaacctt ctggatcacc aaccagcaga cgatgaccgc ccgcaatacc
     8941 atgctgacgg tattttcgcg ccagaccgac aagcagtact acatgaacca gcaacgtacg
     9001 cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt tccaggatgt gctgaatgac
     9061 cctgcgccga agaaactgat catcgttcat ctgctgggta cgcatatcaa atacaaatac
     9121 cgctacccgg aaaatcaggg caagtttgat ggcaataccg atcatgttcc gccaggatta
     9181 agcgcagaag agctggaatc atataacgat tatgacaacg ctaacctgta taacgatcat
     9241 gtggttgcca gcctgattaa agactttaaa gcggcagacc cgaacggatt cctggtttac
     9301 ttctctgacc acggtgaaga ggtttacgac acgccgccgc acaaaactca ggggcgtaac
     9361 gaagacaacc cgacgcgcca catgtacacc attccgttcc tgctgtggac gtcggaaaaa
     9421 tggcaagcga ctcatccccg tgatttctca caggatgtcg atcgtaaata cagcctggcg
     9481 gaactgatcc acacctggtc agatttggcg ggcttatctt acgacggtta cgatccaacc
     9541 cgttcagtgg tgaatccgca gttcaaagaa actacccgct ggattggtaa cccgtacaag
     9601 aaaaacgcgc tgatcgatta cgacactctg ccgtatggcg accaggtagg taatcagtaa
     9661 ttatcagcca ggcgctattt gccggatgcg acgcttgacg cgtcttatcc ggcctacaga
     9721 tcgcggcggt agactgaata aggcgtttac gccacatccg gcaatacaac tgccgcaatg
     9781 tttacatcga actcccctca ggccacactt ttcaggtggc cttttttaat aatgccagtt
     9841 actgttttcc cgtctacata cctaagggaa taacatgtat cacgacgtca gctatctgct
     9901 ctcccgcctg atcaacggcc cgctgtccct gcgccagatt tactttgcca gcagtaacgg
     9961 tccggcgccc gatcttgcgt atcaggtcga ttttccacgg ctggaaatag tgctggaagg
    10021 tgagtttgtt gataccggcg ctggagcaac gttagttccc ggcgatgtgc tgtacgttcc
    10081 cgctggtggc tggaattttc cacaatggca agcccccgct actaccttta gcgtgctgtt
    10141 tggcaaacag caactcggct tcagcgtcgt gcaatgggat ggcaaacaat atcaaaatct
    10201 ggcgaagcaa cacgtcgccc ggcgcggccc acgcataggt tcttttctgc tacaaacgct
    10261 caatgaaatg caaatgcagc cgcaggagca gcaaacggca aggcttatcg tcgccagcct
    10321 gcttagccac tgccgcgatt tgctcggcag ccaaatccag accgcctcac gcagccaggc
    10381 actatttgaa gctattcgcg attatatcga cgaacgctat gcctccgcgc ttacccgcga
    10441 atctgtcgca caggcattct atatttcacc aaactatctc tcgcacctgt ttcaaaaaac
    10501 gggggccatt ggttttaacg aatacctgaa tcacacgcga ctggagcacg ctaagacgtt
    10561 attgaaaggc tatgacctga aggtaaaaga agtggcgcac gcctgcggct ttgtcgacag
    10621 caactacttc tgccgtctgt ttcgtaaaaa caccgaacgc tcgccgtcgg aatatcgccg
    10681 gcagtaccac agccaactga cagaaaaacc gactactcca gaataagatg ggtttgctgc
    10741 ggcgcagaaa gcactttgcg caccgcgctc attacccgct gcggctcacg caggaatgcg
    10801 tagatgctgc attgcacata gcggcaatgt tcaaatcgct cggcaccggc aagctcgata
    10861 tccgtaatca acagagcaac atccgcccga cggatatcct cgtccgctaa acgattctcc
    10921 gttcccagcg cgccctgagt ttcaatgctg acgccccact tctctaactg gcacaacttt
    10981 tccagccgtt ccgccgccat ataagtatgc gccacgccgc ttacgcaggc ggttactgcc
    11041 accaggtatg ccattttaac ctcccacggt aacctgaaat ccggcccgtt ctgccatttc
    11101 gcgcatcgtt gtcacatcgg ctgacgacgg cgcaggcacc tctttcatcg accatgtttt
    11161 ccccagcagg cggtatttcg gttcgccgta ctgatgaaac ggtaacagat ggatctgcct
    11221 gattttcagc gggatcagta catccagcgc ctgctgcata ttctcccggc tgagcgtgaa
    11281 accagggatc aacggtaaac gcgggatcac gttgacaccc tcactcacca gcaaacgcag
    11341 attctccagc acgcgtggca ggttcatctt caccacatcc cgcgcctgag tcgcgtccat
    11401 aatttttaaa tcgaacagca cttcatcgca caatttcgcc agtggtaata gctggctggc
    11461 gcgtctccgg cagtttcaat ggcgcatgac acgccccaca accgcagccg ctgtaaaaaa
    11521 cgggtagcaa actccgcctg cattaacact tcgccgccag aaagcgtgac gccgccgccg
    11581 gacgtgcgaa agaaaatgtc atctttcatc acttcccgtt ccagcgcgtc aaggctgata
    11641 tcgcgaccaa tccgttcaaa cgcaccggag gggcattcat ccgcatcacg caaacatttc
    11701 gcacagtgca gacatttcgc ctctctgcgt actgtctgga ttttgccgga gatcgactcc
    11761 ggattagcac accacgggca aagatgcgga cagcctttaa aaaagaccac cgtacgaatg
    11821 ccctcgccgt cattcagtga ataacgctga atgttgaaaa tgcgcgccac atcatcgcgg
    11881 cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg atgatgtcat cctggatctc
    11941 cttcgacagt tcgacaaaga aggcgctgta tccggcaacg cgcaccacca gcccggcata
    12001 atcttgtggg cgctgttgcg cttcccgcaa cgtgtcggcg ttcaccacgt taaactgaat
    12061 atgttgtaac ttaagctggg taaatgcccg taagaagtcg gccagtttgc gtaatcctgc
    12121 ttcaccttcc agggtcgctg gagtgaattt cacgttcagc aacgtaccgt tagacagcag
    12181 cgtgttatcg agcttactga ctgacttcag taccgctgtt ggcccttgtg cgtcctggcc
    12241 cagcatgggt gacaagccgc cgtctgccag ctgttctccg gcaaaacgac cgtctggcgt
    12301 cgctccaacc accgatccca acgggacgtg agcagaaacg gtatacgatc ccggcgtgaa
    12361 gtagccgccg cgcgggttct ggtatttttc cacttctttg cagtagtggc gtaacagttc
    12421 ggcgctgatg ttatccacct cgtcgatatc gttaccgtat ttctcgaagc ggttaattaa
    12481 gcgagcacgg actttttcgc cttctggcgt tgcaaagttg gcttttaata ccgacagcaa
    12541 ttcgtcaaaa cttaaacgct gttgctcaaa aaccatccct ttcaacgcat ggagagaatc
    12601 gctcaggttg gcgataccga tcccctgtac gccggagaag ttataacgcg cgccgccatc
    12661 ggtaatatcg cggccttttt ccagacaatc gctgataaaa gacgagagca gcggtacagg
    12721 tgcccagtcg cgatggccga tatcacaaat attgctgccc tcaaccatca gggtgatgta
    12781 gtggctgatc ttggcgcgga tctgctccag taaaccttca taagtcagcg cagcattgcc
    12841 ttcattttca tacaggcaga tttccatcac tttcagcaga ttaaacatcg cgatgtcatg
    12901 caagccgtag gttctgccgg gaatagataa ttccacacag cccactacgg aatagtcgcg
    12961 cgcatcttcc agcgaaacgc cacggttgag gaacgctggc accaccacgt catcgttaaa
    13021 gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg gttttcatca ggaacggcgt
    13081 gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc ggtaattgca cgctttgata
    13141 ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta cgtccgttct cggttaaccc
    13201 gccgagcagt gcggtatagc cggtcgggaa accggcgaaa taacgcgcgc tactggtgga
    13261 gcgcaacagc acgatgtcgt tgcatttcac ccataaagat tcgagcagtt ctttcaggaa
    13321 cgccgcatct tcgccctggg ttaatgatgt ctgatagaac ggcaacatat actggtcgaa
    13381 gcgccccaac gatagcgaac tggcgttgga ttcgtattgc agaatgatgt tcatgtacca
    13441 gaataactgg cacgcctgcc agaacgtctg cggcttatgt tgcgcgttat ggcgggagat
    13501 ctccgcaata gtcagcagct cttcgcgacg ctgggcatct gtgcagtttg ccgccatcgt
    13561 ttccgccagt tcggcgtaac gcaaaatgtg tttctgcgag gcttccagca gtaacagcgc
    13621 tgcctgataa aagtgattct ccggctgttg ctgacaatgt tgctgcatct gtgccaccag
    13681 ctcccccagc ccgtgattca acaggcgtgg gtaatcaata ataatgtgcc cctggccttt
    13741 atctgtctgg ttgatgctga aaatctgcgt gctggtcgcg gcttttactt catctgtcat
    13801 ctgcccgttg atgaaatctt tcatcgaacg tttttcccag tacgggaaca actcttcgcg
    13861 gtagatacgt ttatcttctt cgctgatagc aaagcggtcc tgcggacgcg tcgggaattg
    13921 atccagctct ttcagcagcc agtaagggtc catttccggc gacataatcc cggcgcgcgg
    13981 ttttacggtg cggttaccgg caatcagttc ttcatcacga atcgaaattt caacatgttc
    14041 aaggatatac gctgttgcct tcgcccggcg caatatcacc ggttcgcctt cggtttgccg
    14101 atggctggcg gtataaagca gcgcccgctc cagcgagatt tcacgggtat tggaaaacag
    14161 tgcagttttg aggcgagaga tacgattcgt catgaggaac tcctgtggga aagcgaaccc
    14221 gcccttgcga gcgggagaag aacttaagcg gtttgcgcca ggtgagcttc aattttgctc
    14281 ataatggcgt cagcacgttt taccgcatcg ctgatgttga cgcgcacgat ggtcttgcca
    14341 gcaaaacgct cttcaaactt gatgccgata tctttggtca gaatcaccat atcggcgctc
    14401 gccacgtctt ccgcagtcag ttcgttttcc agaccaattg acccctgagt ttctactttc
    14461 acttcccagc ctttcgcttt cgcggcgctt tccagcgcct ctgccgccat ataggtatgg
    14521 gcaacaccag atggacacgc ggttactgca ataattttcg tcatggctcg ttcctcaatt
    14581 aattaatttc aaaatccaaa tccaggtcgt cttccttctc atcagtcgaa gacccatttt
    14641 tacgcgccag acttttcagc acgttcacgc aaacagccgt caccactgct ccgacagcca
    14701 ctgctgcgat ataacccagc ttgccttcaa ccaccggcag cacaatcaga ccaccccagc
    14761 ctgcatagca ctgcgcaccg accagcgccg cagttaccgc accacaaaca gaaccgacca
    14821 tgatggaagg aatgacacgc agcggatcgg cagcggcgaa aggaatcgcc ccttcagtaa
    14881 ccccaacgca ccccatcacc agcgccgctt tgccggtttc gcgctcttct gcggagaaat
    14941 ttttacgacc aatcagcgtc gccagcccca tccccagcgg cgggatacaa atacccacgg
    15001 cagcaatggc caccacggta taaacaccct gagcaacgca aatcagcatg aaggcatagg
    15061 ccactttgtt aaccggaccg cccatatcga acgccagcat cagacccatg atcaccgcca
    15121 gcataacaat gctgccctgc tgcatcccct gaagccactg agtcaggctg ttggtcaacg
    15181 cccctacagg ctcgcccaag ccccacatca tgatgcctgc ggtaatcagt gtgccgacga
    15241 taggaataat gaagataggc atcaccgagc gcagaacttt atgcaccgga attttcttca
    15301 ggtaatgcac cacgatgccg ccgataatcc cggcgatcag cgcaccaaag aagcccgcac
    15361 caaagctgtt accaacccag gcaccgatag cgcacggggc cagcgcagaa cgctctgcaa
    15421 tggagtaacc gatgtaagcg gcgaggaaag gcaccatcag cgtcaaacct gcaacgccga
    15481 tatcaaacag ttttttcaga ttcggatcgg ctacggcatc cggcactgcg cctttgccat
    15541 acaacatgac ggaaaccgcc agcaaaatac cgcccgatac cacgaaggga atcatgtgtg
    15601 aaacgcccgt cattaaatgc tgacgggtat ttttcaggat ctgcaccaac tcattcataa
    15661 gctgctcccg ggcttgcgaa agcccatgtc catatttttg atggaggaaa caataggcaa
    15721 tcgcaggcgg agcaaacagt ggataaaaag gccatttact ggacttttgt aatatcagta
    15781 caaaaatgcg atccgcctca taacttgcga taatccggaa gcatgccgca aaacggcgtc
    15841 ttgtgagacg cgtcgcaaat tgacggttac attacatttg tccagctttt ggcagatttg
    15901 tcacatggca ggccgttgcc gtgcgctctt atcctgtaaa cagaaaaccg taccgggaga
    15961 gaggcaatgg ccctgattgt ggaatttatt tgtgagctac ctaacggcgt acatgcacgt
    16021 ccggcaagcc acgttgaaac gctgtgtaat actttttcat cacaaattga gtggcataac
    16081 ctgcgcactg accgcaaggg caacgccaaa agcgcccttg cgctgattgg caccgatacg
    16141 ctggcgggcg ataactgcca gttactgatt tccggggccg acgaacagga agcgcaccag
    16201 cgtttaagcc aatggctgcg cgatgaattc ccccactgcg atgcgccgct ggcggaagtt
    16261 aaatctgacg aactggaacc actgccggtt tcactgacca atctgaatcc gcaaattatc
    16321 cgcgcccgca ccgtgtgcag cggtagtgca ggcggcattc tgacgccgat ctcttcttta
    16381 gatctcaatg cgctgggtaa tcttcccgca gccaaaagcg ttgacgccga gcaatccgca
    16441 ctggaaaacg gcctgacgct ggtactgaaa aacattgagt ttcgtctgct ggatagcgac
    16501 ggtgccacca gcgcgattct ggaagcgcac cgatccctgg ctggcgatac ttccctgcgc
    16561 gaacatttac tggcaggcgt cagcgccgga ttaagctgcg ccgaagcgat tgtcaccagt
    16621 gcgaatcact tttgcgaaga gttcgcccgt tccagcagca gctacctgca agaacgtgcc
    16681 ctggacgtac gcgacgtctg cttccagtta ctccagcaaa tctacggtga gcaacgcttc
    16741 ccggcaccgg gcaaactgac gcagcccgcc atttgtatgg ctgatgaact gactcccagc
    16801 cagttcctcg aactggataa aaatcacctc aaaggtctgt tactgaaaag tggcggcacc
    16861 acctcacata ccgtaattct tgcccgttcg ttcaacattc cgacactggt tggtgtagat
    16921 attgatgccc ttactccgtg gcagcatcaa acgatttata tcgacggcaa cgctggggcg
    16981 attgtggttg agccaggaga agccgtagct cgttattatc agcaagaagc ccgcgtacag
    17041 gacgccctgc gtgagcaaca gcgtgtctgg ctgacccaac aagcccgtac cgctgacggt
    17101 atccgcattg aaattgccgc taacatcgct cactccgtgg aagcgcaggc cgcattcggc
    17161 aatggtgcgg aaggcgttgg tttgttccgc actgaaatgc tctatatgga tcgcaccagt
    17221 gcaccgggcg aaagcgagct gtacaacatt ttttgtcagg cgctggagtc tgccaacgga
    17281 cgcagcatta ttgtgcgcac tatggacatt ggcggcgaca aacccgttga ttatctgaac
    17341 attccggcag aagctaaccc gttcctcggt tatcgcgctg tgcgtattta tgaagaatac
    17401 gcctcgctgt tcaccacaca actacggtcg atcctgcgcg cctccgctca cggcagcctg
    17461 aaaatcatga tcccgatgat ctcctcaatg gaagagatct tatgggtgaa agaaaaactg
    17521 gcggaagcca aacagcaact gcgtaacgaa cacattccgt ttgatgagaa gatccagctt
    17581 ggcattatgc tggaagtacc gtcggtgatg ttcatcatcg atcaatgctg cgaagagatt
    17641 gatttcttta gtattggtag taatgacctg acgcaatatc tgctggcagt ggatcgcgat
    17701 aacgctaagg ttactcgtca ctacaacagc ctgaatccgg cattcttgcg ggcgctcgat
    17761 tacgccgtgc aggcggtgca tcgccagggc aaatggattg gtctgtgcgg tgagctggga
    17821 gcgaaaggtt ccgtgctgcc gttgctggtc ggcttagggc tggatgaact cagcatgagc
    17881 gcaccatcaa ttccggcggc gaaagcgcgg atggcgcaac ttgatagccg tgagtgccgc
    17941 aagttgctca accaagcaat ggcctgccgt acttcgctgg aagtggaaca cctgctggcg
    18001 caattccgca tgacccaaca ggacgcaccg ctggtcaccg ccgagtgcat cacgctggaa
    18061 agtgactggc gcagcaaaga agaagtgctc aaaggcatga ccgataacct gctgctggcg
    18121 ggccgctgcc gctatccgcg taaactggaa gccgacttat gggcgcgcga ggccgttttc
    18181 tctaccggtc tgggctttag ttttgccatt ccacacagca aatcagaaca cattgagcaa
    18241 tccaccatca gcgtggcgcg tctgcaagcg ccggtgcgct ggggcgatga tgaagcgcaa
    18301 ttcatcatta tgttaaccct gaacaaacac gctgcgggcg atcagcacat gcgcattttc
    18361 tcgcgcctcg ctcgccgcat catgcacgaa gaattccgta acgcgctggt taacgccgcc
    18421 tctgccgacg ctatcgccag cctgctgcaa catgaactgg aactgtaaaa ggaaacatca
    18481 tggaactgta tctggacacc gctaacgtcg cagaagtcga acgtctggca cgcatattcc
    18541 cgattgccgg ggtgacaact aacccgagca ttatcgctgc cagcaaggag tccatctggg
    18601 aagtgctgcc gcgtctgcaa aaagcgattg gtgatgaggg cattctgttt gctcagacca
    18661 tgagccgcga cgcgcagggg atggtggaag aagcgaagcg tctgcgcgac gccattccgg
    18721 gcattgtggt gaaaatcccg gtgacttccg aaggtctggc agcaattaaa atactgaaaa
    18781 aagagggtat tactacgctt ggcaccgcag tgtacagtgc tgcacaaggg ttactggccg
    18841 cgctggcagg ggcaaaatac gttgctccgt atgttaaccg cgtagatgcc cagggcgggg
    18901 acggcattcg tacggttcag gagctgcaag cgctgttaga aatgcacgca ccagaaagca
    18961 tggtgctggc ggccagcttt aaaacgccgc gtcaggcgct ggactgttta ctggcaggat
    19021 gtgaatccat caccctgccc ttagatgtag cgcaacaaat gctcaacacc cctgcggtag
    19081 agtcagctat agagaagttc gaacacgact ggaatgccgc atttggcact actcatctct
    19141 aaaggagcaa ttatggaccg cattattcaa tcaccgggta aatacatcca gggcgctgat
    19201 gtgattaatc gtctgggcga atacctgaag ccgctggcag aacgctggtt agtggtgggt
    19261 gacaaatttg ttttaggttt tgctcaatcc actgtcgaga aaagctttaa agatgctgga
    19321 ctggtagtag aaattgcgcc gtttggcggt gaatgttcgc aaaatgagat cgaccgtctg
    19381 cgtggcatcg cggagactgc gcagtgtggc gcaattctcg gtatcggtgg cggaaaaacc
    19441 ctcgatactg ccaaagcact ggcacatttc atgggtgttc cggtagcgat cgcaccgact
    19501 atcgcctcta ccgatgcacc gtgcagcgca ttgtctgtta tctacaccga tgagggtgag
    19561 tttgaccgct atctgctgtt gccaaataac ccgaatatgg tcattgtcga caccaaaatc
    19621 gtcgcgggcg cacctgcacg tctgttagca gcgggtatcg gcgatgcgct ggcaacctgg
    19681 tttgaagcgc gtgcttgctc tcgtagcggc gcgaccacca tggcgggcgg caagtgcacc
    19741 caggcggcgc tggcactggc tgaactgtgc tacaacaccc tgctggaaga aggcgaaaaa
    19801 gcgatgcttg ccgccgaaca gcatgtagta accccggcgc tggagcgcgt gattgaagcg
    19861 aacacctatt tgagcggcgt tggttttgaa agcggcgggc tggcggcggc tcacgcggtg
    19921 cataacggcc tgaccgctat cccggacgcg catcactatt atcacggtga aaaagtggca
    19981 tttggtacgc tgacgcagct ggttctggaa aacgcaccgg ttgaggaaat cgaaaccgta
    20041 gctgcactta gccatgcggt aggtttgcca ataactctcg ctcaactgga tattaaagaa
    20101 gatgtcccgg cgaaaatgcg aattgtggca gaagcggcat gtgcagaagg tgaaaccatt
    20161 cacaacatgc ctggcggcgc gacgccagat caggtttacg ccgctctgct ggtagccgac
    20221 cagtacggtc agcgtttcct gcaagagtgg gaataaccta ctccaaactc ccggcttgtc
    20281 cgggagtttg aacgcaaaat tgcctgatgc gctacgttta tcaagcctac gaaatctctg
    20341 caatatattg aatatgcgtg cttttgtagg ccggataagg cgttcactcc gcatccggca
    20401 ctctgtgcca gtaatctgaa actcccacgt tgttcaggag ttttttatcc gcttctggac
    20461 attttctcca cagaacaatc gttagcccgg aaggtcgaat ctgtgactct tattttcacg
    20521 gacgaaccag atgaaagctt cactggcgct gctcagcctg ctaacggcat ttaccagcca
    20581 ctccttaaaa tctcccgccg ttccaccaac cgtggtacaa atccaggcca ataccaacct
    20641 tgccatcgca gacggtgcca gacagcaaat cggcagcacg ctattttacg atccggcgta
    20701 tatgcagctt acctatcctg gcggtgatgt cccgcaagaa cgcggggtat gttccgatgt
    20761 ggtgatccgt gcattgcgca gccagaaggt cgatttgcag aaactggtgc acgaagatat
    20821 ggcgaagaat tttgccgagt acccgcaaaa atggcagtta aagcgcccgg acagcaacat
    20881 cgatcaccgt cgggtgccta atctggaaac ctggtttacc cgccacgata aaacacgccc
    20941 catcagcaag aaccccagtg actatcaagc gggcgatatt gtctcctggc gactggataa
    21001 cggactggca catattgggg tggtgtcaga tggcttcgcc cgcgacggta cgccgctggt
    21061 gatacataac attggcgcag gcgcgcagga agaagatgtg ctgtttagct ggcggatggt
    21121 ggggcattat cgttattttg ttaaataagc gatcgcccca catgaagaaa aaattcagat
    21181 gctttttaca ctgacagctt cttttttctt acggctcacc agcgccagtg ccagcacaat
    21241 cagcacaata ccgctacctt caacggcccc cggattctca ccgagcagcc accaggaaaa
    21301 gagtacgccg caaacgggaa cggccagcgt gcttaagctg gcaatactgg caggcaagtt
    21361 tttcaataca aacaaccata agctccacgc cagcgccgtc gccagaatcg cactgtaggc
    21421 cagcgcccag aacacggtgg gctgccagtc aatttcacgt tgcggcacca gtaaagcgac
    21481 cacactcatc accagcgccg catagagcat ctgccaggat gttaacgata ataaatccac
    21541 gcgcggatga cgggtgtata ggcgtttagc aacaatcgcg ctcgcccccc aactgacgcc
    21601 ggagaggatt gccagcatgg cacttttcat tgaagagaaa tcgagttgcc acggctgcaa
    21661 caccaaaaat aagccgaaag cggcaatcag tatcgcgaaa tattgcccac gtcgcaggcg
    21721 ttcaccgaga aacaacgcgg cgaaaatcac cacccagaac ggcatggtat agcttaggat
    21781 cgccaccttc cccgctccgc cgctgaccag cgcccactgc gccaggccaa ccatcccgca
    21841 ggtttgtaac agggcaatgg ctaaggtgta tttaaacggt gtcgggcgca ttccgcgacc
    21901 acgtaataaa aggacgatga ataaaacgag agcgccgaaa atgcagcgta aggcggtaaa
    21961 gtcgaaggca ccgatgtagc tggtgacttg cttcatgaaa atccagctat aactccagat
    22021 aagtgtgagc acaaccaggc cactgattgc cagtgggttg ctctttcctg cggcagacat
    22081 gagagatcca gtgtgtagtt ttcctgtgga cactatacgc cggtaaagaa acttcagcga
    22141 ctgcctgaaa ggcagtcgct gaagaaggtc agattacagc aggtcgaaac ggtcgaggtt
    22201 catcactttc acccatgccg ccacgaagtc tttaacaaac ttctcgtgag catcgctgct
    22261 ggcgtaaact tcagccaccg cacgcaggac agagttagaa ccaaatacca gatccgcacg
    22321 gctggcagtg tatttcactt cgccggtttc gcggtcacgg ccttcaaaca gctctttcga
    22381 ttcgtcggtc gctttccact cgtaacgcat atccagcaag ttcacgaaga agtcattgct
    22441 caatacgcca acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt tggcacccag
    22501 tacgcgcatc ccgcccacca gcgcggtcat ttccggcgcg gtcagggtca gttgctgggc
    22561 tttgtcgatc agcagtgact cggtggtgga aacatccaga cgagcgcgat agttacggaa
    22621 accgtcagca attggctcca gcagctcgaa catctcaatg tcagtctgat cctgacgcgc
    22681 atcaacgcgg cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc ttgcggcttt
    22741 ctcaacacca accacaccag ccagcacgat gatatccgcc agcgaggctt taccagactc
    22801 tttctggatt ttctccagaa caggcagagc acgaacggct gcggcgttca catcccagtc
    22861 gcgctgcggc attaatgcca gacgcgcacc gttggcacca ccgcgtttgt cgccaccacg
    22921 gaaggtagaa gcagaagccc aggccaccga taccagctca ctaacagaca gaccagaatc
    22981 cgcaatcgcg aatttcagat cgataatgtc ctgctcggtc gggttgtaga tcggctgcgg
    23041 cagcggatct tgccagatca gatcttcttt cggcacttcc ggcccgatgt agcgagattt
    23101 cggccccata tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt cgttgaacgc
    23161 ctgcggatcg ttgaggaaac gacgagagat cttctcgaac tcaggatcaa aacgcagcgt
    23221 caggtcggtc accagcattg tcggtttacg tttcttcgac ggatcgaacg gatccgggat
    23281 aatttccggt gcgtctaccg cttcgaactg gattgctcca gccggactgc gggtctgtac
    23341 ccactcatac ttgaacagat tctcgaagaa atagttgctc cactgggtcg gcgtctgggt
    23401 ccagactact tccagaccag aggtaatggc atctgcgcca acgccgctgc cgtaagtgct
    23461 cgcccagcct aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac ctacgtttga
    23521 tgtcggaccg gcaccgtggg ttttacccag cgtatgacca cccgcaatca gcgccacggt
    23581 ttcttcgtcg ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg ccgcagaaag
    23641 cggttcgccg ctgtgatccg ggccttccgg gttaacgtaa atcaggccca tctcggttgc
    23701 acccagcggt gctttcgcca gcgcttccgg atgacggtga gtcagccagg ctttttcatc
    23761 accccagtta acatccagat ccggttccca gacgtcttca cgaccggcac caaaaccgaa
    23821 ggtacggaag ccggagtttt ccagcgccac gttacctgcg aggataaaca ggtcggccca
    23881 ggagattttc tgaccatatt tctgtttgat tggccacaac aggcgacgcg ctttatcgag
    23941 gcttacgtta tccggccagg agttcagcgg tgcaaaacgt tgctgaccac gacccgcgcc
    24001 gccgcgtcca tcgattgaac ggtaagtacc tgcgccgtgc caggccatac gaataaacag
    24061 accggcgtag ctgccccagt cggctggcca ccacggctga gattctgtca gcagggcttt
    24121 cagatctttt ttcaggccgt agtaatctaa tttgctgaat tctttgcggt agtcaaagtc
    24181 ctcacccagc gggttagaac gattagaatg ttggtttaac aggtcaacac ggagttgatt
    24241 tggccaccag tcgcgagtgg ttgtgcccgc ccccgcgctc tggtcgtgac cgccctgatg
    24301 gaacgggcat ttgccagtgg ctgtcgtgtt atggatatcg tctgacgtgc tcatcaatgt
    24361 gctcccctct acagtgttac cgttacgata cacagcgtta gagagaagtt ataattgaat
    24421 taatccacgg atgcgatagt tgagatctta catattgttg gttaaagaga tgtagatcaa
    24481 attgatcgta attaagcttt tttgattatg tccgaattcg gacaactgct ttataaaaaa
    24541 gggttttaga ttcctattct tcaaggaagg agggcgaaaa caaagctgac aaccttattt
    24601 ttacgccctc atttcgaggc agcattttgt gctctgttta aaatttgtga tcacagtgtg
    24661 attttcacaa aagccacact atttataaac caggtcgaac acccagcgta tggcaaatcg
    24721 cgtaactcat ttcagcgcgg ttaagcgtat agaagtggaa atctttcact ccttcacggc
    24781 ttaaaatctt caccatatcc atggcaatat tcgcgcccac cagtttgcgg gtttcggcat
    24841 catcatccag gccgtcgaac atttgcgcca tccacgccgg aatacgcacg ttggtcatat
    24901 cggcaaattt cttcgcctgt ttaaagttag ataccggcaa aattcccgga ataatttcca
    24961 catcaatgcc cgccgataca cagcggtcac gaaaacgcag gtagctttcg acatcgaaga
    25021 agaactgagt aatcgcgcgg ttggctccgg catcgacttt gcgtttcaga ttaagcaaat
    25081 ccgcctgagc gctttttgct tccgggtgaa cttccggata cgccgccacg gagatatcga
    25141 aatctgccac ttcttttaat agcgtcacca ggtcagaagc atacatttct ggcttaccac
    25201 ttcccggcgg cagatcgcca cgcagcgcca cgatatgacg aataccgtta ttccagtagt
    25261 cgcgtgcaat ggtgcgcaac tcgtcgggcg tcgcatcaat gcaagtaaga tgcggtgccg
    25321 cttccagacc agtgcgatct ttaatgcctt taataatgct gtgcgtacgg tcgcgctcgc
    25381 cggagttcgc gccataggtc accgatacaa acttcggttt caggctgcta aggcgatcga
    25441 tggagttcca cagggtctgc tccatttcac tggtacgcgg cgggaaaaac tcgaacgaaa
    25501 cgttaatttg cccctggact tctgccaggc tctgattcag ggcatcccgc tggctggcgt
    25561 gaaaaaagct cataccttac ctcatcaatc gcttgtagtt atatgttgtg ttgtgcacat
    25621 ctatccgttt agacgtccag atgtaaagat gaaggaaaag ccgaagggcg tcaaccgaaa
    25681 aatgaccaat gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg atggtaagaa
    25741 aaagaaaatc cggtatgcaa agcaaaccgg actgcaaata taaactaacc atgtacttat
    25801 tgcttcttgc cggattcgtc gtaaacgctt aatccggcct aatcctatga taattcaata
    25861 tatttcatta gaaaccataa acccgaaaac atgagtaccg ggcattatta aatttctgaa
    25921 attacaacaa ctgtgccagc cggttgatat ccgactgaat cgccccggcg gtgacgtcgc
    25981 gccccgcgcc aggcccacgg atcaccagcg ggttgtcgcg ataccagcgg ctttcgatgg
    26041 caaagacgtt atcgcacggc agcagtgatg ccagcggatg gtcttcacgc accgcctcca
    26101 cgcccacacg cgctttgccg ttggcatcga aacgcgccac gtagcgcagc accagcccca
    26161 tttcgcgggc cgcttccagc cgttgcacca tctgctcgtt aagttcatcg ccattttcaa
    26221 agaaatggtc gatgctgccg ccttcgcaat gagcaggcac cagcgattcc acacgtacct
    26281 ggtccggttc aatgttgtaa cctgcttcac gcgccagaat taccagcttg cgcattacgt
    26341 ctttgccgga gaggtcgtca cgcggatcag gctcggttaa gccctgctgc cacgcctgat
    26401 ccaccagctc ggtaaacggc acgctaccgt cgaattgcag gaacagccag gagagcgtgc
    26461 cggagaagat cccgctgatc gacaaaatag tatcgccgct gtcgatcaga tcgcgcacgg
    26521 tgtggttgat cggcaagccc gcaccgacgg tggcattgta cagccagtga cgcccggttt
    26581 tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gctggctccc gccagtttgt
    26641 tggcgctgat gacgtggaaa ccgtggctgg cgaaatcaag atactgatca gcaagctgct
    26701 ggctggcggt aacgtccagc accactaaat catcatacgg atgggcgcgc atccacagga
    26761 acaacgactc ttcatcctgc tcaaccgctt catcgttgaa gaaggctaac gcgcggctgg
    26821 cgtccagccc gtcatagctc aacaggctgc ggcggctgtc caccacacct gccagcacaa
    26881 actcaaagcc ggtacgtgcc gaaagcgtgc tctgctcacg ggcgaacagt tccagccaac
    26941 gggaaccgat attgccctta ccgaacaata ccaggccgat gcgtttttct gcgcggaaga
    27001 cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca
    27061 ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg ctggcctttc agttgctgcc
    27121 agaagcggtg gcaatgcagc gggttacggg tgacgcctgc accgaccatc gccaccagcg
    27181 ccagcccctg acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg attttcagcg
    27241 cactgtcggc cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg ttatgtacgc
    27301 caaccgccag cgggcgtacc tgcgcgcgtt tcaggatttg gtcgatctct ttatgcgcca
    27361 gtttgaaatc ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg
    27421 tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat
    27481 ccggcgtgta gctacagcgc agttgcaggt cgatttcgct gccagaaacc ggctgtaaag
    27541 tacgggcgtg aagaacggga gccgccaggc gcgccagttc gctggcctca tccagacgca
    27601 gcaacggcag caggcaggca tctttcactt tacgcgggtc ggcactgtat accccggcga
    27661 cgtcgctcca gatggttacg cgagaaacac ccgccagcgc accgatttgt gtcgcggaat
    27721 agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttgcgg ctgataaatc
    27781 cggtcaccac cagacgtttg cccggatgtt gcaccagcag ctgttgcagc aacgggtaag
    27841 aaagcccttc atcaacctgc ggttgtgcgg cgcgttcagc gcgtaaaaac tcgcgggcat
    27901 caagccaggc cgctggcagc ccttgttgat taagtaccgc agacatcagg cgtgccgacc
    27961 atacttcccc gtggcccacc acttccgcat acactgcgtc gttaataccg ctgtcgagca
    28021 gcgccgccag gcgctcaagg tcgctgacaa aagcgctaat gaggctatcg gcttcttcag
    28081 cgggtagcag accgctaatc agatcgcact gatagcgacg cagcgtttgt tgaacctgat
    28141 gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc
    28201 taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc ataatgcccg
    28261 cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta tgcagctgac
    28321 gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa
    28381 gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgcag
    28441 cagcgtctcg gaaatcccgg cggcagcacg cgcttctggt gccatgcctg catgtgtcat
    28501 ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa
    28561 cgacagcccg cccaggaaac gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa
    28621 cattgcgcca aagccttttt gctgacgcgc ggcaatttca tgcccctgat tttccggcag
    28681 cgacgggtga tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg
    28741 cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gtagcagcag
    28801 atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt
    28861 gacaacgtcc gggtctttag caatcaccac gccggccacc acgtctgagt gaccgttcag
    28921 atatttcgtg catgaatgca acaccagatc ggcacctaat gccagcggat tttgtaatgc
    28981 cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca
    29041 gattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag
    29101 tttgggttta tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat caacaaacaa
    29161 cacgcgatag caaccgcgtt tcgccaggct gtcgaacagg cgatagctac cgccgtagca
    29221 gtcgtgcggc gcaaccagca gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat
    29281 cgcggacatg ccggtattag taagtactgc accagcaccg ccttccagtt ctgccagcgc
    29341 acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg cgcgcggttc
    29401 attaaatccg gtaaagttat atgtgctgga aagatggatc ggtgggacaa cgcaaccata
    29461 ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg
    29521 atgaagttcc ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat
    29581 ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat
    29641 agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtaac
    29701 gcctgtacgg taaactatgc gggtttacgg tcagtaccca cataaattgt gtggtctggt
    29761 ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc
    29821 agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt
    29881 cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg
    29941 cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa
    30001 cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca
    30061 aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcggaaaa
    30121 gagcggcgcg gagttaacca gccgcttttt tcatacaagg acgtttaacg atgaaaatca
    30181 ggctactgat cctcagcctg ctggtttctg tgcctgcctt tgcctggcag ccgcaaactg
    30241 gcgacatcat ctttcagatc tctcgctcat cgcaaagtaa agcgatccaa ctggcgaccc
    30301 attccgatta tagccacacc ggtatgctgg tgatgcgtaa caaaaagccc tacgtttttg
    30361 aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat ggtgaaaagg
    30421 gcaaatacgt cgttcgccgc gttgaaggcg gactgagtgt tgaacaacag caaaaactgg
    30481 cgcaaacggc aaaacgttat cttggtaaac cgtatgattt cagcttctca tggagcgacg
    30541 atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg ggaatgcgcg
    30601 tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc caggcgaagc
    30661 tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca ccacaagccg
    30721 ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt tcgtggtaat
    30781 ggatgatgaa aaaaataatt cccgccgtag tctgtttgtt gctttgtagt tgcacgacct
    30841 ttccgcaagc tgtcgctccg gtaaacggtg atgcctccaa ttagttgaat taaggatata
    30901 atactcgctt ttaaggttgt ttcatggtca cagttaacct tcattgcccc cgttgccagt
    30961 ccgttcaggt ttaccgtcac gggcagaacc caaaaagaca tgatcgattc cgcggccgtg
    31021 actgtcaccg ggtatttcag ctcagttact gttacgagcc ccgtaaaccg ggagttaaag
    31081 accaaatcgc tgaaatgact ttcaacggtg ccggggttcg cgatactgcc cgtacgctga
    31141 aaatcggcat taatacagtc atccgaactt taaaaaactc tcgccacgaa gaataacgtc
    31201 atccccggtc gctcatgctg atgtggcact catctgcaaa cttagtcagc aatggagctt
    31261 tgtcggcagt aaagcgcgac agcactggct ctggtacgtg tacaacacga aaactggtgg
    31321 tgttctggcc tacacttttg gcccccggac tgacgaaacc tgtcgtgaat tgcgggcgct
    31381 gcttaccctt ttaccatcgg catgttgacc actgacgaat ggggtagtta cgctagagag
    31441 ttaccgaagg agaagcatct gactggtaag atttttactc agcacattga gcgtaacaat
    31501 ctgaccctga gaacccgaat caaacgtctg gcccgcagaa ccatctgttt ctcgcgttct
    31561 gtagaacttc atgaaaaagt catcggtgct tttatcgaaa atatatgttc tactaattgg
    31621 agtcatcacc cttctgataa gctttccttg gatgattacc agactgaata cacagccaaa
    31681 gatttttacc tcattctgct cttttttctc aaatgacata tattatattt tacatcatat
    31741 tcataggcat atgacattcc cccgcccccc attatgtcat tgtgg
//
LOCUS       DAZFFI010000049        31397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFFI010000049 DAZFFI010000000
VERSION     DAZFFI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.049"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..1112
                     /gene="folD"
                     /locus_tag="ACHJ12_002601"
     CDS             246..1112
                     /gene="folD"
                     /locus_tag="ACHJ12_002601"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4990369.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1114..1326
                     /gene="ybcJ"
                     /locus_tag="ACHJ12_002602"
     CDS             1114..1326
                     /gene="ybcJ"
                     /locus_tag="ACHJ12_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4990370.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1434..1955
                     /gene="ybcI"
                     /locus_tag="ACHJ12_002603"
     CDS             1434..1955
                     /gene="ybcI"
                     /locus_tag="ACHJ12_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4990371.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1991..3376)
                     /gene="cysS"
                     /locus_tag="ACHJ12_002604"
     CDS             complement(1991..3376)
                     /gene="cysS"
                     /locus_tag="ACHJ12_002604"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4990372.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3550..4044
                     /gene="ppiB"
                     /locus_tag="ACHJ12_002605"
     CDS             3550..4044
                     /gene="ppiB"
                     /locus_tag="ACHJ12_002605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4990373.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4047..4769
                     /gene="lpxH"
                     /locus_tag="ACHJ12_002606"
     CDS             4047..4769
                     /gene="lpxH"
                     /locus_tag="ACHJ12_002606"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4990374.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4887..5396
                     /gene="purE"
                     /locus_tag="ACHJ12_002607"
     CDS             4887..5396
                     /gene="purE"
                     /locus_tag="ACHJ12_002607"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4990375.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5393..6460
                     /gene="purK"
                     /locus_tag="ACHJ12_002608"
     CDS             5393..6460
                     /gene="purK"
                     /locus_tag="ACHJ12_002608"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4990376.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6597..7490)
                     /gene="ybcF"
                     /locus_tag="ACHJ12_002609"
     CDS             complement(6597..7490)
                     /gene="ybcF"
                     /locus_tag="ACHJ12_002609"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4990377.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7487..8302)
                     /gene="ylbF"
                     /locus_tag="ACHJ12_002610"
     CDS             complement(7487..8302)
                     /gene="ylbF"
                     /locus_tag="ACHJ12_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4990378.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8313..9572)
                     /gene="ylbE"
                     /locus_tag="ACHJ12_002611"
     CDS             complement(8313..9572)
                     /gene="ylbE"
                     /locus_tag="ACHJ12_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4990379.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9582..11249)
                     /gene="fdrA"
                     /locus_tag="ACHJ12_002612"
     CDS             complement(9582..11249)
                     /gene="fdrA"
                     /locus_tag="ACHJ12_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4990380.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11566..12615
                     /gene="allD"
                     /locus_tag="ACHJ12_002613"
     CDS             11566..12615
                     /gene="allD"
                     /locus_tag="ACHJ12_002613"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4990381.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12637..13872
                     /gene="allC"
                     /locus_tag="ACHJ12_002614"
     CDS             12637..13872
                     /gene="allC"
                     /locus_tag="ACHJ12_002614"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4990382.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13883..14668
                     /gene="allE"
                     /locus_tag="ACHJ12_002615"
     CDS             13883..14668
                     /gene="allE"
                     /locus_tag="ACHJ12_002615"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4990383.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14797..15942)
                     /gene="glxK"
                     /locus_tag="ACHJ12_002616"
     CDS             complement(14797..15942)
                     /gene="glxK"
                     /locus_tag="ACHJ12_002616"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4990384.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15964..17265)
                     /gene="ybbY"
                     /locus_tag="ACHJ12_002617"
     CDS             complement(15964..17265)
                     /gene="ybbY"
                     /locus_tag="ACHJ12_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO4990385.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTVLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17322..18683)
                     /gene="allB"
                     /locus_tag="ACHJ12_002618"
     CDS             complement(17322..18683)
                     /gene="allB"
                     /locus_tag="ACHJ12_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO4990386.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18743..20197)
                     /gene="ybbW"
                     /locus_tag="ACHJ12_002619"
     CDS             complement(18743..20197)
                     /gene="ybbW"
                     /locus_tag="ACHJ12_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4990387.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTVEKTGEQKTIG"
     gene            complement(20367..21245)
                     /gene="glxR"
                     /locus_tag="ACHJ12_002620"
     CDS             complement(20367..21245)
                     /gene="glxR"
                     /locus_tag="ACHJ12_002620"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4990388.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21345..22121)
                     /gene="hyi"
                     /locus_tag="ACHJ12_002621"
     CDS             complement(21345..22121)
                     /gene="hyi"
                     /locus_tag="ACHJ12_002621"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4990389.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22134..23915)
                     /gene="gcl"
                     /locus_tag="ACHJ12_002622"
     CDS             complement(22134..23915)
                     /gene="gcl"
                     /locus_tag="ACHJ12_002622"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4990390.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24005..24820)
                     /gene="allR"
                     /locus_tag="ACHJ12_002623"
     CDS             complement(24005..24820)
                     /gene="allR"
                     /locus_tag="ACHJ12_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4990391.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24898..25380)
                     /gene="allA"
                     /locus_tag="ACHJ12_002624"
     CDS             complement(24898..25380)
                     /gene="allA"
                     /locus_tag="ACHJ12_002624"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4990392.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25610..26536
                     /gene="allS"
                     /locus_tag="ACHJ12_002625"
     CDS             25610..26536
                     /gene="allS"
                     /locus_tag="ACHJ12_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4990393.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26605..27699
                     /gene="mnmH"
                     /locus_tag="ACHJ12_002626"
     CDS             26605..27699
                     /gene="mnmH"
                     /locus_tag="ACHJ12_002626"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4990394.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMQKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27815..28180
                     /gene="ylbG"
                     /locus_tag="ACHJ12_002627"
                     /pseudo
     CDS             27815..28180
                     /gene="ylbG"
                     /locus_tag="ACHJ12_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28947..29194)
                     /locus_tag="ACHJ12_002628"
                     /pseudo
     CDS             complement(28947..29194)
                     /locus_tag="ACHJ12_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29175..29883)
                     /locus_tag="ACHJ12_002629"
                     /pseudo
     CDS             complement(29175..29883)
                     /locus_tag="ACHJ12_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(29886..30254)
                     /gene="ybbC"
                     /locus_tag="ACHJ12_002630"
     CDS             complement(29886..30254)
                     /gene="ybbC"
                     /locus_tag="ACHJ12_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HFO4990395.1"
                     /translation="MKYSSIFSMLSFFILFACNETAVYGSDENIIFMRYVEKLHLDKY
                     SVKNTVKTETMAIQLAEIYVRYRYGERIAEEEKPYLITELPDSWVVEGAKLPYEVAGG
                     VFIIEINKKNGCVLNFLHSK"
     gene            complement(30294..>31397)
                     /gene="rhsD"
                     /locus_tag="ACHJ12_002631"
     CDS             complement(30294..>31397)
                     /gene="rhsD"
                     /locus_tag="ACHJ12_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO4990396.1"
                     /translation="QPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFP
                     AELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKAHLYHCDH
                     RGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQPYRLPGQQHDEESGLYYNRHR
                     YYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLLQTWDDARSGACTGGVCGV
                     LSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVCKDTNGKYFASKAETDNLR
                     KESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDEFFSSSDKNLVRSKDNNLEAFYLAT
                     PDGRFEALNNKGEYIFIRNSVPGLSSVCIPYHD"
BASE COUNT         7811 a         8459 c         7589 g         7538 t
ORIGIN      
        1 ttgtcaggta tttcttaaat tatcttaatc cttaaacaag gaaataaatc agttccagat
       61 ttacaacgcc atcatggacg aaaaatgaag ctttcaatct cagcgacctc agcgacggtg
      121 cgcctaacct tcgcaagagg tcgcttcacg cgataaatct gaaacgaaac ctgacagcgc
      181 gccccgcttc tgacaaaata gacgcatccc cttcgaccta cgtaacagat ggaatcctct
      241 ctctgatggc agcaaagatt attgacggta aaacgattgc gcagcaggtg cgctctgaag
      301 ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg ggcaccagga ctggccgttg
      361 tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc aaggcttgtg
      421 aagaagtcgg gttcgtctcc cgctcttatg acctcccgga aaccaccagc gaagcggagc
      481 tgctggagct tatcgatacg ctgaatgccg acaacaccat cgatggcatt ctggttcaac
      541 tgccgttacc ggcgggtatt gataacgtca aagtgctgga acgtattcat ccggacaaag
      601 acgtggacgg tttccatcct tacaacgtcg gtcgtctgtg ccagcgcgcg ccgcgtctgc
      661 gtccctgcac cccgcgcggt atcgtcacgc tgcttgagcg ttacaacatt gataccttcg
      721 gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg tcgcccaatg agcatggaac
      781 tgttgctcgc aggttgcacc actaccgtga ctcaccgctt cactaaaaat ctgcgtcatc
      841 acgtagaaaa tgccgatctg ttgatcgttg ccgttggcaa gccaggcttt attcccggtg
      901 actggatcaa agaaggcgca attgtgattg atgtcggcat caaccgtctg gaaaatggca
      961 aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac attacgcctg
     1021 ttcccggcgg cgttggcccg atgacggttg ccacgctgat tgaaaacacg ctacaggcgt
     1081 gcgttgaata tcatgatcca caggatgagt aacatggcga cattttcttt aggtaaacat
     1141 ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct ggagcgaaag cggcgcgcag
     1201 gcgaaaatcg cgattgccga aggccaggtg aaagtcgacg gtgcggttga aacgcgcaaa
     1261 cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag gtcacagcgt acaggttgtt
     1321 gcctgatccc tttcccggcc tgtatcaaca ggccggttct cttttcagac ttttgccctc
     1381 tacgccccgt tcatccatgc tgatagcatc gacattttgc cgaagcgacc atcatgccaa
     1441 ccgttattac gcacgccgcc gttcccctat gcattggttt agggttgggt tcgaaagtca
     1501 tccctccgcg tctgttattt gccggaatca tcctggcgat gctgcccgac gccgacgtat
     1561 tgtcgtttaa atttggcgtt gcttacggca atgtttttgg tcatcgcggg tttacccatt
     1621 cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct gattggacgc cgatggttca
     1681 gggctgggct gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttgc
     1741 tggattcggt aaccactggc ggtaagggcg ttggctggct gtggccgtgg tcagatgaac
     1801 gctttttcgc tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca
     1861 cgccgtacgg gcatcaggtg ataatttctg aattgatgtg ggtgtggttg ccggggatgg
     1921 tgcttatggg aatgttgtgg tggcgcaggc gataaccgga tgcgaaaact cgcatccggc
     1981 aacagcgcaa ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc
     2041 ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc ccagtctttc gctttacggg
     2101 catccagacg ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg
     2161 cgccgctttg caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt
     2221 tacgcaggtg agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttta
     2281 cttcacgcgc catatcaaac agtacggaat aggcttccgg ggtgttgaaa tcgtcgtcca
     2341 tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg
     2401 tgccgcgcag cgcagtgtag agacgctcca gcgccgcacg cgcctgcttc aggttctctt
     2461 cgctgtagtt caactggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg
     2521 cgtcgtagta tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct
     2581 tctcgcggtc aaccatcacc attccggagt gcatccagta gttcacatac tgaccatcat
     2641 gggcacaggt ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc
     2701 cgccgtggat atcaaagtgg ttacccagct gcttgcagtt catggccgaa cattcaatgt
     2761 gccagccagg acgacccgcg ccccacggag acggccagct cggttcgccc tctttcgaca
     2821 tcttccacag aacgaagtcc atcgggttgc gtttgtcatc aaccacgtca acgcgcgcgc
     2881 cagcctgcag ctggtcgaga tcctgacgcg acagcacgcc ataagttgga tcggtcggga
     2941 cgtcgaacat cacgtcgccg ttgtccgcca cataagcgtg acctttggcg atcagttgtt
     3001 cagtgagttc aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca
     3061 gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca
     3121 caaagctttc gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt
     3181 tgcgcacata cttcagttta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag
     3241 caacaaaggt acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacgt
     3301 acatgccgac ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat
     3361 tgaagatttt tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata
     3421 atacccatat ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat
     3481 gctataacac caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag
     3541 gatgcaaaaa tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac
     3601 gataaagcac ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac
     3661 aacaccattt tccaccgtgt tatcaacggc tttatgattc agggcggcgg ttttgaaccg
     3721 ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa
     3781 aatacccgtg gtacgctggc aatggcacgt actcaggctc cgcactctgc aactgcacag
     3841 ttcttcatca acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt
     3901 tggggctact gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa
     3961 ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc caaaagaaga cgttatcatt
     4021 gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct
     4081 gcgtggaaga accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca
     4141 aggccgacgc gctgtatatt cttggcgatc tgtttgaagc atggattggc gacgacgatc
     4201 ccaacccact ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc
     4261 cctgttattt cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa
     4321 gcggcatgac attattgccg gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga
     4381 ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag
     4441 tccacaaacc ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca
     4501 ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag cagtaaatcg ctggcgatca
     4561 tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa acatcaggtg caatggctga
     4621 tccacggaca tacccatcgc ccggcggtac atgaacttat cgccaaccag caacctgctt
     4681 ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggcggatg
     4741 acgttgagct gattcatttt ccgttttaaa aaacccacaa ctttgctgat ttcacagcca
     4801 cgcaaccgtt ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt
     4861 tgtgcaccac aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca
     4921 tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt cgccgccgaa atcttcgaaa
     4981 tcctgaatgt cccgcaccac gttgaagtgg tttctgctca ccgcaccccc gataaactgt
     5041 tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggtg
     5101 gcgcagcgca cctgccaggc atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc
     5161 cagtacagag cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc
     5221 gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcattac
     5281 tggcagcaca aattctcgcg actcacgata aagagctaca ccagcgtctg aatgactggc
     5341 gcaaagccca gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca
     5401 ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt
     5461 aggcattgct gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca
     5521 aagcgtgatt accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc
     5581 gcgtcatccg gcctttgtga accgcgatgt gttcccgatt attgctgacc gtctgactca
     5641 gaagcagctt ttcgataagc tccacctgcc gaccgcgccg tggcagttac ttgccgatcg
     5701 cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac
     5761 tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc aatgaaaccg aacagttacc
     5821 ggcagagtgt tacggcgaat gtattgtcga gcagggcatt aacttctctg gtgaagtgtc
     5881 gctggttggc gcgcgcggct ttgatggcag caccgtgttt tatccgctga cgcataacct
     5941 gcatcaggac ggtattttgc gcaccagcgt cgcttttccg caggccaacg cacagcagca
     6001 ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat
     6061 ggcgatggag tgttttgtca ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt
     6121 gcataacagc ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct
     6181 gcgggcgatt accgatctgc cgttaccgca accagtagtg aataatccgt cggtgatgat
     6241 caatctgatt ggtagcgatg tgaattatga ctggctgaaa ttgccgctgg tgcatctgca
     6301 ctggtacgac aaagaagtcc gtccggggcg taaagtgggg catctgaatt tgaccgacag
     6361 cgacacatcg cgtctgactg cgacgctgga agccttaatc ccgctgctgc cgccagagta
     6421 tgccagcggc gtgatttggg cgcagagtaa gttcagttaa gcaggtgctc atttgattgc
     6481 cggatgcggc gtaaacgcct tatccggctt accgctccgg tacccattgt aggcctgata
     6541 agacgcgtca agcgtcgcat caggcatggt gcaccgattg ccggatgctc tcatcactac
     6601 agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca
     6661 atccacgcag gtttaccacg gcttctgaca taaccactca ccgccgttac cttcggcccc
     6721 atcgaaccat cggctttggc aaatggcgct aactcatccg gtgtggcatg gcgaatggcg
     6781 cgttgctgcg gcgttcccca gttttcatat accgcatcag catcagtgag gatcaccagc
     6841 ccatcggcgt taatctgctc agcgagcaac gcagcagcga gatctttatc aatcaccgct
     6901 tcactccctg ccccatcttc cgtcacaggc acaccgccgc cgccactgca aatcaccaca
     6961 tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga
     7021 gacgccacca cccggcgcaa atatttacca tcacgtttca tctgccagcc gtaagccgct
     7081 tccagtgcct cttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa
     7141 aacgccggat catcaggcga aacctcaatg cgcgtcagca ccgtcgtcac gggcggcatc
     7201 tgcggctgtg cgctcaaact ctgcgccagc atatagccaa tcatcccctg actttctgcc
     7261 accagcacat ccagcggata cggttctacc tctttccacg ccagattctg caatgccagc
     7321 agccccacct gcggcccgtt gccgtgaaca atcgccagcc gataagaacg ggccaggcgt
     7381 gccagcgcgg gtacagcact ggcgatattg cgatattgat tttccgccgt cagcgcctca
     7441 ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt
     7501 gtaataattg ttggccaagc cagaacccca gcagcgtatc tgcccctgac gtatgcccca
     7561 gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa
     7621 agtgcaggag tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca
     7681 ccgctgtggt aacgagttga agattgtcgg aacaggcaaa aaactggcga ccggagcgcg
     7741 catctaaagc gccataatac caggccgcca gcagcattcc gctcagcgtg tcatcatgac
     7801 tcggcgttaa tccggggcct ttacccagcc agtgccgcca gtcggtctta acgccattga
     7861 gcgcggcctg aaaacagtga cgaaactggc gtaactcagc aggcagcgga tcgcttgccg
     7921 ccaacgccag tggcccgaaa agcccggttt cctccgcgcg ttgcatccat gcagctgcaa
     7981 gtggttgagg atgtgaaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa
     8041 ccgtgaaacg cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata
     8101 atccaccgca taacgcatcg aattgcgcac ggcgaagcat ccatcctccg gggccgaaac
     8161 cactaccctg acgatgcagc gttaacagtt cgccgctttc cgtcatcagg ttaatcgccc
     8221 gtcgccacac tcccgctaac cgccaggact ggcgataatt cggtgcacta cggctggcaa
     8281 gcagaggatg gatgatcgtc attgcgcgtt cctcaaccaa tccccatgct ttcagccagt
     8341 gcttccagcg cctgttcaaa acacgccagc ggtgcccgca cagtgcctgc gccaatctgc
     8401 ccgatccccg cctctttatg ggcgataccg gtattgatga gtggcgtaat gccggtttct
     8461 accacgcgac gaatgtccag tcccaggcac gcgccctgaa aatcccagcc tgggatctgc
     8521 aactgcatat tgcgctcaag gtaaatttcc gccatctctt cagataccgc tctggccgct
     8581 tccatgccac ccgcaccgac aaagcgcgtt acgccaggcg ctgcgatcat tgccgcacct
     8641 ccgataccaa aggtttcggt aatcgcgcta tcgcccatat ccgggttcgc ctgctcctgc
     8701 gagaagccgg tgaaaaacag accttgcgga gtgtttacag gcgcagtaaa ccagcgttcg
     8761 cccagcccgc ttacccgaat cccgaacata ttgccgttgc gggtcattgc cgtgacgatg
     8821 ctgcctgcgc ggatcatcgc gccagcatcc atcgccgcct tgcagtaagc catcgcgagg
     8881 ttgaggaaga actgatcggt cacgctgagg aaatccatca cttcggcgat gtgctgttta
     8941 tcatgatcga ggcgagcaat ttgtggggcc agcgcacgca ttaacagtgc agaggaagca
     9001 atattgcgtt gatggaactc atcgcccatc gtaatgccct gcgccatcat cgccgtgaga
     9061 tcgataccgc gctccatgcg ccccagcgcc gcgcttaata ctggcattaa cacatcgcgc
     9121 atccagcgat ggcgagtcag gacatcttcg ccgtaagcgc caaaacgcat cactttgccg
     9181 ataccttcgt tgaggttgca gtacgcccgg ttgccgtcgg tcacgttctc gaccaccagc
     9241 atcggcatac tggcagaagt aataccgccc attggaccga cggcattcac atggtgacaa
     9301 ggaatgaagt tcacttcccc ctgctccagt attgccagcg cctgcgcttc atctttcgcc
     9361 cagccttcga acagacatgc gcccacgcac gcccctttca tgggtccggt catctcctgc
     9421 cagcgcattg gcggcccggc gtgaagcagt gttttgccct cgtttagttc gctgataagt
     9481 gaagaagccg gttgcacatc cagccagtgt ggacgagcgc gacgaatttg ttcgataacc
     9541 gcagcattgg cttgcgccac tgatgtaaac atgggaaccc cttattgcaa acgttctaat
     9601 aaacgagcca gttttttatt gccgccggcg actggcgacc attggtaatg cacaaccggt
     9661 ttgctggcgc tttgtagctc cagcgcaaag ctgcgtaatc cgatgttaat cacggcgacg
     9721 ttttccagta atgacggtgt gtgttgctga gtggcaggcg aaagcggacg aatcagtgcc
     9781 gccgccagca aggtggcttc cggcagcgaa ctcactaccg cgatccctgc atcttccagc
     9841 gtggcgattt gctgcgagcg gcattgcggg tcacgttcgg tacctgtcac cgtggcaatg
     9901 gcgtacagcg gttggctgtc tgaacgcgcg gcacaggctt tttgccaggc gctcaccagc
     9961 gaggcagcag gatcggcggt cgcaccgaag ccaatcacga catcaagcag caacacgcgc
    10021 acttgcggtt tagcgccgag atcggcaatt aactggttgc gtaaggctgg gtcgatcatc
    10081 ggatggggac gcccgacggt gtagaaatca tcgcccagat caatgatctg gtgaccatcg
    10141 gcgtccagca tcatgccgtg atgatgggta tcgtcggctt ccacgccaag gtgtccagca
    10201 agtaatcccg ccgcttcggc agccagcgta ccgccggtat acaaaccgca aataaatccg
    10261 ctgctggcag gcgttatcgc gttacgtcgc gccgtaacgc gtgaaagcag gcaagccagg
    10321 cgtgcggcct catccagcga ggaggcaaac cagacattct cgtcgcgtgc caccgccggg
    10381 gtataaccta aaaacagcgc caccgtcggt ttgccggttg ctttcatggc attaacaatt
    10441 ttcagacgca cagcttcggc aggtggtttt gaaacaaatg ccagcacttc gcttttctcg
    10501 tctgcactga gcatttccag cgccgtcagc gcactgatgc cgcccacttc acggctgagg
    10561 tcgcgcccgc caaggccaat cgcgtgagta attccctccc ctgccagcgc aatctgtgag
    10621 cacagctcct gaatcccggt accggaagcg ccaatgacgc caatgttgcc ttccggcatc
    10681 acgttagcaa aagccagcgg cgtggcggca atcatcgatg taccgcagtc cggccccatc
    10741 accagcaagc ctttttcccg cgcgcgggtt ttgagttgga tttcatcttc cagcgtgacg
    10801 ttatcggaga acatcatcac gttgaggttg cgatccagcg cctggttcgc cagctccgcc
    10861 gcatactcgc cagccactga aatcagcgcc agactggcat cgggtaattt ctgacaggcg
    10921 ctgtcccagc gacgcacctg cgtcaaggac tggctgctgc ctgacccctg cgccagttgt
    10981 tttagcgcct cttcaagctg ctgcataatc gcctgcgcga tccccgcatc cgccgcttcg
    11041 ctacgaattg ccacgcaaat atcgttcggc gtggcgttat taaaatcgtc atgccagaaa
    11101 cctgtggtat ctaataacgc tttattggcg ggcgtaccca tcattacgga aacatcatca
    11161 acattttctg attcgctgag ttttcgtgaa ataatcatta aactgaccga atcctgaaaa
    11221 cacccttttt taataaaggc gtggatcata acaactcctt aaaaattgca cccatggcaa
    11281 ttgcacgagc agtgttaatg tggcgattac ggtatcgatg acaacaagta aataaggtga
    11341 tgaatatcac acaggaatta ttaacaaaag aattcaaata accgccaggc tattacgcat
    11401 tattttttaa taagcgtggt tgttttaaaa atcaggttaa aagtgagcgg attatcaaat
    11461 aaattctaac tatatttttt tgcctgtctg gatcacataa tccagatatt ttccctgtat
    11521 gttaattgca gtcatgcttc acaccgtcgt taaaaaggaa gacagatgaa aatcagtcgg
    11581 gaaacactcc accagctaat tgagaataaa ctctgccagg ctgggttaaa acgtgagcac
    11641 gctgcaaccg tggctgaagt attggtttac gccgatgcca gagggatcca ctctcatggc
    11701 gcggtgcgcg tggaatacta cgcggaacgc atttcaaaag gcggcaccaa ccgcgaaccg
    11761 gagtttcgtc ttgaggaaac cgggccgtgc tcggcaattt tacatgccga caatgccgcc
    11821 ggacaggtcg cggcgaaaat gggtatggaa catgccatca aaaccgccca gcaaaatggc
    11881 gttgcggtgg tcggtatcag ccggatgggt cacagcggcg caatctctta ttttgtgcag
    11941 caggcagccc gcgccggatt aattggtatt tcgatgtgcc agtccgatcc aatggtggtg
    12001 ccgtttggcg gcgcggaaat ttactacggt actaacccac tggcctttgc cgcgccggga
    12061 gaaggcgacg agatccttac ctttgatatg gcgactaccg tacaggcatg gggaaaagtg
    12121 ctcgacgccc gctcgcgtaa tatgtctatc ccggatacct gggcggtcga taaaaacggt
    12181 gcaccaacaa ccgatccgtt cgcggtacat gctctgctcc ccgccgccgg gccaaaaggg
    12241 tatggcctga tgatgatgat tgacgtcctc tcaggcgtct tactcggctt accgttcggg
    12301 cgacaggtta gttcgatgta tgacgattta cacgccgggc gtaatttggg gcaattacat
    12361 atcgttatta acccgaactt tttcttctcc agcaaattat ttcgtcaaca tcttagccag
    12421 accatgcgcg aattaaatgc cattaccccc gcgcccggtt ttaatcaggt ttattatccc
    12481 ggacaggatc aggatattaa acaacgcaaa gccgccgtcg aaggcatcga aattgttgat
    12541 gatatttacc agtatttaat ttccgacgcg ctttataaca cgtcatacga aacgaaaaat
    12601 ccctttgcgc aataattatt gagacaggac tttcttatga ttacacattt tcgtcaagcc
    12661 atagaagaaa cgctgccctg gctttcctct tttggcgctg acccaacggg tggtatgacc
    12721 cgtttacttt attcgccgga atggctggaa actcagcagc aatttaaaaa aagaatggca
    12781 gcaagcgggc tggaaacacg tttcgatgaa gtggggaatt tatatggtcg cctgagtggc
    12841 accgaatatc cacaagaagt ggttctgagc ggttcgcata tcgataccgt ggttaacggc
    12901 ggtaacctcg acgggcaatt cggcgcgctg gcggcgtggc tggcaattga ctggctgaaa
    12961 acgcaatacg gcgcgccgct acgtacggtc gaagtggtgg cgatggcaga agaagaaggc
    13021 agccgcttcc cgtatgtctt ctggggcagt aaaaatatct ttgggctggc gaatcctgac
    13081 gacgtgcgga atatctgtga tgccaaagga aatagttttg tcgatgcgat gaaggcttgc
    13141 ggatttactc ttccgaacgc cccactaact ccgcgtcagg atattaaagc ctttgttgaa
    13201 ctgcatattg aacagggctg tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat
    13261 gcaattgtcg ggcagcgtcg ttatacggta acgctgaacg gcgaatcaaa ccatgcaggc
    13321 accacgccga tgggttatcg tcgtgataca gtttacgctt tcagtcgcat ttgccatcag
    13381 tcggtcgaaa aagcgaaaag gatgggcgat ccgctggttc tgacctttgg caaagtagag
    13441 ccgcgcccga atacggtaaa tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt
    13501 cataccgacg ctgccgtgct gcgcgatttc acccaacagt tagaaaacga catgcgggcg
    13561 atttgcgatg aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg
    13621 ccgatgaata aggagctggt cgccaccctg acagaattgt gtgaaagaga aaaactgaat
    13681 taccgggtga tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca
    13741 acctgcatga ttttcattcc cagcatcaac gggatcagcc ataacccggc ggaacgcacc
    13801 aatattaccg accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc
    13861 tggcagaaat aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgatga
    13921 tttactggct aaccgtgcga ttgttaaaca cggtaatttc gcactgttaa ccccggatgg
    13981 tctggtaaaa aatattattc cgggctttga aaattgtgac gcgacaatcc tctccacgcc
    14041 aaagctgggt gcctcttttg ttgattatct ggtcacactg catcaaaacg gtggcaacca
    14101 acagggcttc ggtggcgaag gcattgaaac gttcctgtat gtgatctctg gaaatatcac
    14161 tgccaaagcc gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccacc
    14221 aggctcctta atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa
    14281 gcgccgctat gttccggtag aaggccatgc accgtggctg gtttctggca atgccagcga
    14341 actggaacgc attcactatg aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa
    14401 agagttaggt tttgatatga acatgcatat cctctctttt gcaccaggtg ccagccacgg
    14461 ttatatcgaa acacatgttc aggaacacgg tgcctatatt ctttccggtc agggggttta
    14521 taacctcgac aataactgga tcccggtgaa aaaaggcgat tacatcttta tgggcgctta
    14581 ttctttacag gctggttatg gtgtagggcg tggtgaagcg ttcagctata tttactcgaa
    14641 agattgcaac cgcgacgtag agatttaagt cataccactc gcctgattat ttttagctat
    14701 gttggatttt gccacggttt ataccgtggc tttttttgta gtaaattcag gtaaaaaaag
    14761 atgcgatata aatcgcatcc gctttaaagg taagggtcag tttttaattc cctgacctat
    14821 tttaatggcg caggcaatat ttcgtgcgct gttgaagaga ttggtttcac cgctggcgag
    14881 cacttcggct aaaggtgcca gacgtggcaa aatgctgaat accgcatcaa tgccgtactg
    14941 gtgcaccact tccacgccat cacccaatac gccagcaatc ccaatcaccg gtacattaaa
    15001 ctgcttcgcc accgacgcca cacccagcgg cgctttaccg cctgccgttt gcgagtcgat
    15061 gcgcccttcc ccggtaatca ccagtgctgc gccctgcact gcctgcgcaa gattgaccgc
    15121 attcaacaca atctcaatac ctggtttaat atccgcattg agaaataccg ccgccgcaat
    15181 acccataccg cccgcagcgc cgccgcccgc catctggcag acgttgattt cagtttgctg
    15241 ttgtaaaaca cgggcgtaat tttgcagccc ctgttccagc tcttcgacca tttccggcgt
    15301 tgcccctttt tgcgggccaa acaccgccgc cgcgccgcgt gcccctacca gcgggttatc
    15361 aacgtcacat gctacttcaa tatggcaatt cgccagacgc ggatcgcatt catccatctc
    15421 aatgcttgcc acgcgcgcta aattaccgcc gttagccacc agcacctgac cgtcggcatc
    15481 aaggaaacgc acaccgagcg cctgcgccat gcccatacca ccgtcgaccg tcgcactgcc
    15541 accaatgccg agaataatat ggcgaatgcc gttatccagc gcatgacgaa ttaactcccc
    15601 cgtaccaaaa ctggaggcca gcaacggatt acgcttttca ggcgcgacca gcatcaggcc
    15661 acttgctgcc gccatctcaa tcaccgccgt tttcccgtcg ccggtaaggc cataaaaagc
    15721 attcactttt tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc cgcccgtcgc
    15781 ggcgaccatc gcatccaccg tcccttcgcc gccatccgct atcggcaaac agatatagtt
    15841 cgcatcggga aagagggtcg aaaacccggc tttaattgcc tgacaacatt tttctgcact
    15901 taagctctct ttaaaagagt ctggcgcaat gacaatcttc attatcaatc ctttttcaac
    15961 aaattattcg atgcgttccc acggaataag attatccata agaacagcca gtaaaatccc
    16021 gaccagtaag ccgttactga gcagaggacg cagcgttaat ggaaggtctt gcagatacac
    16081 aggcggtaat gccattaaaa atatgccgac aaataacggc aatgcgagtc gataaatatt
    16141 gcgagcggta aacgttattt gctggctaaa caccagcgcg gaaaagagta aaggcagata
    16201 agaaaccagc atgaccgcac tactcacggg taaagggata ctgcaaaaca gtcgtgtgag
    16261 tgccggaacc agcgccacca gaaggcaaat aacgctgcca taaataaatg aacgccgcgt
    16321 gtaatcgcca gtctgggtta ataaaccaat ggatgaaaca aacggtgaaa atggaattac
    16381 cgccagaggt acggttatca gtgtcataaa tccggtcgcc acaaagctac gacgataacg
    16441 cgtattcccc gcgccctgct gcggataaaa aacatccgtg ccccgaatcg caccgtaggt
    16501 attgctgata tttaccagac ctgtaatcac cgccgtcaga ataattcccg gcgacaaagc
    16561 accgccactg ccgagcggga accactgcca gtgcaactca ccggagagcg agtgcgaaga
    16621 aggaaagcaa aagtaccaca acaaccagcc ggttatggtg ccgaccagca ggccataacg
    16681 ggcaaaacgt tgcgggagga agataatcat ccccagtacc aggcacatca ccgccaccga
    16741 gagcgcgaac ggcggtaact gaattttaaa attcgggtcg gctatgccaa acggcagccc
    16801 gagcatacct ttgaaaaaga tagtggtcag ctgcgcgccc agcatcaaca taaacaagac
    16861 catcaccgac ggcgtaaata accgcgccag gcgatggcct aatccgctaa aaccaatcaa
    16921 caccgtcagc acgccggaga gtgcaatccc cactgccagg ctggtggcga tatcgttgat
    16981 cggtgtcccg cgcgatgctt cgccaagggt gatagtaagg atggttcccc accacaggcc
    17041 acctggccct tccataatag cgcgacgatg accgcagaac gcctgagcga agcaggccag
    17101 tgcggtagca agaaaagcat attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa
    17161 agcagaaagt agcgtaggag gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg
    17221 aaatcctgat aacaggcttt cgcggctcac tgcaaaattg aacataaaca caccttaacc
    17281 ggagaatgcc cgccgcaagg acgggcattg caggggccag attactgctg atgtttaagg
    17341 ataaattgac ctttcggcgc aacagggaag ccctgttcaa tgtcgtaaat cacatcacca
    17401 cgtaagatgg ttttcgtgat acgcgcgcca atggtacggc caacatacgg gctgactttg
    17461 tggcgatatt ccagatcgtc attggtaaga acatagctgc tattcggctg aatgaagacg
    17521 aagtcggcat cttttcctgg ggcgatacgg cctttttgct gcagaccgaa aatatctgct
    17581 gcgttagtcg ccattaattt gccgaacatt ggcagagaca ttccgcgttt ctgtaccgct
    17641 tcatcgaaca tcacgtccat acagttttgc aggccggcaa taccgcccca tgcttccatg
    17701 atgttgccgg ctttcatttc cggagggcat ggtgagtggt cggaaaccag gcagtcgatt
    17761 tcaccgttaa acagtttttc ccacatgcct ttctggtttt ccagatcgcg gatcggcggt
    17821 gaacacttcg ccagagtgcc gatttcttcg aactgatcgg tatccagtac aaagtaatgc
    17881 gggcaggatt cacaagtaac gtcctgacct tcctgacgtg cacgagtcac ttcttcaacg
    17941 ccttccgggc tgctgatatg gcaaacgtgc agacggcaac cggcaacttt cgccagatac
    18001 agcacgcggc gaatcgcttc cacttcggta aataccggac gcgaagccac atagtcatgg
    18061 gcagttacgc gaccttcacg tttcgcttct tcgcccagtg cgtcacagat cagcgcgttt
    18121 tcgcagtgca ccagcaccgg ctgccccagt tcgcccagct tctgcgcacc tttgaagaac
    18181 tgccagtcgt ttacatcacg gaagtcgttg tcgataccgc gatcgccaca ggtcgcaacg
    18241 aagcatttga agccgacaac gcccacttca tccagctcat gcagacggtc gatgttgtaa
    18301 gacaccaggc caccgagttg tgccgcatcg atagtcagct tgcctttagc ggcatcgaac
    18361 ttcagttcaa ttgaagcgcg gtcaaccgtt gcaggcagct ggttgagcgg catttcgatc
    18421 atggtggtga taccaccttt tgccgctgcg cgagtaccgg tttcataacc ttcccagtgg
    18481 ctacgacccg gttcagaaat atgggtgtgc gcatcaacca tgcccggcga aaccaccaga
    18541 ccagacgcat ccataacttc ttttgcatcg cccagatcct gaccgatagc agcaattttc
    18601 ccgcctttaa cggcgatatc tacaacgcga gcttcgtttt ctaaaataac ggtgccgttt
    18661 ttaatgatta aatcaaaaga cataacaaac tccttgtaaa atacagattg gtttttaact
    18721 caatgttcaa tatcgggatt aattaaccta tggttttttg ctctcctgtt ttttctactg
    18781 ttgtacgttt ctttaataag gcgtaggccg caaaggcgac gatgacgccg acaaaccatg
    18841 aaacacgcga taacggttcc ataaagggaa taaacttacc gccaagagat aaaataacgg
    18901 ccaccagagt tactgaaaac gcggtgaggt taaaaccgtt atcgtaatat ttataatcgc
    18961 caggtgcggt gtacagttca tcaagattaa tttgcccacg catcaccaca aaataatgtg
    19021 ccatcatgac accaattacc ggacccagca ttccgccgat gatatcgagg aacagataaa
    19081 tactgtcctg attttccatt aatttccacg ggcagatcag caggctgata atactggcaa
    19141 tcagtacgcc gtttttatag gtcagttttg tcggtgcaat ggcagcaatc tgataaccgg
    19201 ccggaataat attaccggtg gcgttagtgg aaatagtcgt catcagaata accagtaccg
    19261 caaagaacga ggcaaacagg ctgtcccaac gctgaacaat atccagcacg ttccaggtgt
    19321 cagcgccgta gtgaatactg gctccggcaa taatacatac cccagcgacc gcaaacagaa
    19381 tataggccac aactaaaccc agcgtttgcc ccagcgcttg ctcacgaaac gagtgggcgt
    19441 tttgcgtaaa gtcggatgcg ctcaccgccg gtgccgccca gaccgcaact accgcgttaa
    19501 tcaccaccag gaacaagaat ccactgtttt ctgctttctg aataccgctc ggaatgtagt
    19561 caaagattgg accgatcccg accagtgaaa tcgcccaaat cgccatgccg ccgaaaacga
    19621 tatagatgca cgggttaaga atggcagtga atttatttaa aactttgcca ccgccaaaac
    19681 caataccaac gttaaccagc cagaagagta agaaagtaat taagcccggt agcgaaaggc
    19741 ctaacaaggt aaaatcacca ccgagagtta aaaatcccgg ccagattttg ccaatcagga
    19801 tcaagcaggc cagtgatccg gcgtaacatt gcaggccaaa ccacataatg gcagcaatac
    19861 cacctcttaa taatccggga aacagtgcgc cacgtacgcc gtaagaagca cgcaggatca
    19921 tggcaaacgg tacgccgtat ttactgcccg cagcaccgtt taataccatt accgcggcaa
    19981 tgaaaaaggc gctgaggata attgccagca taatactaaa ggtagacaag ccgagaataa
    20041 aaaagccgcc gaccatcaca taattgggaa cgttatgaac cgaacccatc cataaggtaa
    20101 aatagttaaa tgttttccag gtccgctggc tttgcgtttt cggcaataaa tcttcgctat
    20161 agccgcgttg ctggaatagc tttctctgat gttccataaa tccctcttaa tttcacgtat
    20221 cgaataacgt tgttttaatc tagctatgag gaacaaataa aataaaaaat attattttta
    20281 atactgaagc aacatctttg gctgctttct tttttaatat tgctggccga ttcattttct
    20341 gatatcggcc atttttattg cgggtatcag gccagtttat ggttagccat taattccagc
    20401 gcctgcacta acgcagagtg atccaactgg ctgccaccgt ttgccgcaca ggtattaaat
    20461 aactcctggc aggtcgcagt gtttggcagg ttcagcgcaa gtgctttcgc actttgcagt
    20521 gccaggttga gatctttctg gtgcagagcg attttgaagc ccggattaaa ggtgcgttta
    20581 atcatacgct cgccatgaac ttccagaata cgtgaggaag caaagccgcc catcagcgcc
    20641 tggcgcacac gtaccgggtc cgcaccggct tttgaagcaa atagcagggc ttcagaaacc
    20701 gcttcaatat tgagcgccac aataatctga tttgccactt tgcaggtttg accatcgccg
    20761 ttaccgccca cgagggtgat atttttaccg agcagttcaa acagcggttt aacacgttca
    20821 aataccgctt catcaccgcc aaccataatc gacaacgtcc cttcacgcgc accgatttca
    20881 ccgccggaga ctggcgcatc gagataatcg ccgcccagtt cattcacctg acgagcgaaa
    20941 cgcttagttt caatcgggga aatggagctc atatcaacaa tggttttgcc cttcagcgag
    21001 gctttggtac aaccattttc accgaacaga acttcttcaa cctgtggtgt atctggcacc
    21061 ataataaaaa tgatgtccga tgcttccgtt acctggcgcg cagtttcaac actgacggca
    21121 cccagtgaca gtaattcatc agcaaccggt ccaatggttg tgacatgcag ttgatgaccg
    21181 gcacgcgcca gattaatggc catcggtgta cccataatgc ctaagccaat aaatcccagt
    21241 ttcataatta acctctttta aatttcgctt ttcctgaatt cagacaacac gatcccctgc
    21301 caacttagcg gcctaaaaaa gcattgcctg aacagcgtta cgttttaacg gtacggatcc
    21361 atccagcgta aaccggcttc cgtggtggtt tggggtttat attcacaccc aacccaaccg
    21421 ttgtagtcag aattttcgat taccttaaag agataatcat aattaatttc tccggttccc
    21481 ggttcgccgc gatgcggatt atcggcaatt tgcaggtgac caattttatc agcccactga
    21541 gtcatggtgt tggttaattc accttccatc cgctgcatgt gataaatgtc atactgaatt
    21601 ttcaaattgc agcaaccaac atcatcaatc aatttcagcg cctgccgagt tccggtgaga
    21661 tggaaaccag gaatatcaaa atggttaata ggttcaatca gtaataaaat atcttctttc
    21721 atcagcatat tcgcggcata acgcaggttt tctacaagcg ttgcgtgaat ctgttcactg
    21781 ctgaaaccag ccggcgtttt accgaccaga cagttaattt ttttattacc cagcgcacgg
    21841 gcataacgaa tcgctgctgc tacgccatcc cgaaactctt cttcacggcc aggaatacag
    21901 gcaataccgc gctcccccgc cgcccagtca cccgccggta aattgtgcag cgtatgttcg
    21961 agtttattac tcgccagcac ctgttttaat tcttcaatgt cgtagtcata aggaaacata
    22021 aattcaacgc cgcgaaaacc acactgcgca gctttctcaa aacgggcgag aaaatcatat
    22081 tctccaaata acatcgataa attagcagag aaacgtaaca ttatttatct cccttattca
    22141 tagtgcatga agcaggtttc agtcggtgcg tccgctgcgt tatcggcgat atcttcaaat
    22201 tccatgacgt tatccagttc gctgcccatc gaaatattgg tcacacgctc gagaataact
    22261 tccacgacta ccggtacccg atattgcgcc attaaggctt tcgcctgttc aaaggctggc
    22321 gcaatatctt ccggtttgaa gacgcgaatc gctttacaac ctaaaccttc cgctactttt
    22381 acgtggtcaa caccgtagcc attcacttca ctggagttga tattctcgaa agcgagttgc
    22441 acgcagtagt ccatatcaaa cgcgcgctgc gactggcgaa tcagccccag ataagcgtta
    22501 ttgaccagta catggatgta cggaatgttg aactgtgcgc caacggccaa ttcttcaatc
    22561 aggaactgga agtcgaagtc accggaaatc gccaccactt tgcgctctgg atcagcggca
    22621 caaaccccca gcgccgccgg aatggtccag cccagcgggc ctgcctgccc acagttgatc
    22681 cagtggcggt ctttaaagac atgcagcatt tgcgcagcgg caatttgcga cagaccaatg
    22741 gtggtgacat agcaaacatc gcgaccaaag gctttgttca tctcttcata cacgcgctgc
    22801 ggtttcaccg gcacgttgtc gaaatgggtt ttgcgcagca aagtgcgttt gcgctgctgg
    22861 cagtcggcga cccattcttt acgacacggc agacgacccg ctttttgcat ctcctgcgcc
    22921 acttcaacca gcagtgtcag cgccgcttta gcatcagaga caatgccgag atccggacac
    22981 agcacgcgac caatttgcgt cggctcaata tcgatatgaa cgattttgcg cccttcggtg
    23041 tatttctcta ccgaaccggt atgacggtta gcaaaacggt taccgatacc aaacaccatg
    23101 tcggacgcca gcaacgttgc gttaccgtaa cgatgcgcgg tttgcagacc caccatcccg
    23161 gccatcagtt catgatcgtc cgggatacag ccccagccca tcagcgttgg gatcaccgga
    23221 acgctggtca gttcagcaaa ctgttgtaac agtgcagctg cgtcagcatt aattaccccg
    23281 cccccggcaa caatcaccgg acgttcggcc tggattaaca tttctacagc tttttcgatc
    23341 tgcatacggc tggcagcagg tttgtagacc ggcagcggtt cgtacatgtc aggatcaaac
    23401 tcgatttccg caacctgaac gtcgaacggt aaatccacca gtaccggacc cggacgacca
    23461 gaacgcatca ggtgaaatgc ctgttgcagc acacgaggca ccagcgccgc ttcacgtact
    23521 gtaaccgcca ttttgctgac cggcttggcg atagcttcga tatcaacagc ctggaaatct
    23581 tctttatgca gacgggcgcg cggagcctgg ccggtaatac acagaatagg aatggaatca
    23641 gcagaagcag aatagagcgc ggtgatcatg tccgtgcccg caggaccgga agtccccaga
    23701 catacgccga tattccctgc cgttgcgcgg gtataacctt ccgccatgtg cgaagcacct
    23761 tccacatgac gcgccagaat gtgacgaata ccgccgtgct tacgcatcgc tgagtagaac
    23821 ggatttattg cagctcccgg aacgccgaag gcggtggtga taccttcttt ctccagcaca
    23881 tacattgccg cgtcaacggc tctcattttt gccattttat tcctacctct attaattgga
    23941 aaatttttcc aactttcaaa ttttgtcaaa aaaacccgct ccgcagagcg ggatgcgaca
    24001 gacattatgg atgtgctttc agtcccaacg ccgtgctgat atcgcgggcg gtgtctctga
    24061 ccagctcacc ctgactgaca aaacgatctt ctgtcagtct tgatgaaggc ccggagatgg
    24121 agatagcggc aacaacacta cctacatcat cgtaaattgc tgaagctaag caattcagac
    24181 ctacaacatg ctcttcttta tctacggtat agcccagttc acgcgcttgt tccaggtcct
    24241 tcagcaaggt gggcatatcc acaagcgtag ttggcgtaaa ctgctgcaaa ccggtttgca
    24301 gaatgatgct catcaactcc tcttccgcca gcggataaag cagcgctttg cccgcaccgg
    24361 aagcatgcag cggcagacga ctgcccagtg gcgcacacat cctgaccatc gatttacact
    24421 ctaactgacc aattaatacc gcttcattgc cgttacggat cgcgacattg accgtttcgc
    24481 cggaaagtaa catcaggcgg cgcataaacg gcccggcgac ggagaggaca tcgcggttat
    24541 ggatgtacgc tgcaccgacg ttaaagacac ctaatcctat atgccaccag cctaattgac
    24601 tgtcctgata gacaaaatcc gctgcctgta aaaccttcag caagcgaaag gtcgtggaga
    24661 gcggcaaatc cagattgaga gaaatatcgc taaccgacga acttccccca cttttttcca
    24721 aatattgcag aatcgcaatt ccccgctcta acgcctgtgc gcccttctgt gccacaggct
    24781 ccgcctgtcc tggcctgccg cgccgtctaa cttccgtcat acctacgtta actccgacct
    24841 acaagtgagc ataaacaatg aacaattagt ttatcttatg caagtcggtt gcaggcgtca
    24901 cgcaaaacag agttcctgtt caggaatact ttcaacatca cagttgtcac tgccgccgcg
    24961 atcgatggtc agaaaatcgg tgacgcgctg ccaggcgaaa agtgggtgat gccagacgtt
    25021 acgatggtaa ttcactccct gttcgccgtt ggtgataaac gcccgcagcg ttgacaggtc
    25081 tggtttgtcg tcgcctaacg ccacgaccac cacaaacact tcacctttca tcgggataaa
    25141 ggcctgagtg cccagcggat gacgttcgag ttcgtgaatg gtcagcggca gattcgccgg
    25201 ttgcgcgcgg ttaatgctga taagcgtacg gtcttgctca agaatctcaa ccagcgccaa
    25261 atcgtggtaa cgctccacca ggccattgtt aatatggaaa aaatcccgtt gctgcgtttc
    25321 gattacgtcg ccataagcac taaaggcttc ctgacttaac ggtaatacct gaagtttcat
    25381 ctttttgtcc ttgtaacata attccgttca accagcgccg accggtcagc ttggtttcaa
    25441 taacaggcaa tctaaaacct taagagaaca acatcaatac aaaagttgga atatttttcc
    25501 aaaactagat ttagtccaca aattgaacag ggttatcatg taaaaatact gataaatcag
    25561 aggataagaa aatcacgcaa cttgcggatt taaaagggga tacctgaaga tgttcgatcc
    25621 agaaaccttg cggactttca ttgcggttgc tgaaacagga agtttttcaa aagcggcaga
    25681 acgattatgt aaaaccacgg cgacgatcag ttatcgcatt aaacttctgg aagagaatac
    25741 cggagtagcg ctgtttttcc gtacgactcg tagcgtaacg ttgacagcgg ctggcgagca
    25801 tctactttcc caggccagag actggctgag ctggctggaa agtatgccaa gcgagctgca
    25861 acaggtgaat gatggcgtgg aacgccaggt gaatattgtc atcaacaacc tgctctacaa
    25921 cccccaggcc gtcgcccagt tgctggcgtg gctgaatgaa cgttacccct ttacccagtt
    25981 tcacatctcc cgacaaatct atatgggcgt ctgggattcg ctattgtacg aaggtttttc
    26041 actggctatc ggcgtcacgg gaaccgaggc gctggcaaat acctttagtc ttgatccctt
    26101 aggatcggtg caatggcgct ttgtcatggc ggcggatcat ccgctggcga acgttgaaga
    26161 gccgctaaca gaagcgcagt tgcggcgctt tccggcggtc aatattgaag acagcgcccg
    26221 caccttaacc aaacgcgtcg cctggcgatt gccagggcaa aaagagatta ttgttcctga
    26281 tatggaaacg aaaatcgccg cccatcttgc gggcgttggc attggttttt tgccaaaatc
    26341 gctttgccag tcaatgatcg ataatcaaca actggttagc cgggtaatcc caacgatgcg
    26401 ccctccttcg ccattgagtc tggcatggcg caaatttggc agcggcaaag cggtagaaga
    26461 tattgtgacc ttgtttaccc agcgcaggcc ggaaatcagc ggatttttag aaattttcgg
    26521 caacccacgc agttaaaaat caagtttcct tgcgcgttgg cgcgtaaaat agcgcgcaag
    26581 ctgatttgag gttcactttt aactatgcaa gagagacaca cggaacagga ctatcgtgcc
    26641 ctgctgattg ctgatacgcc cattattgat gttcgcgccc ctatcgagtt tgagcaaggc
    26701 gcaatgcccg ccgctatcaa tctgccgtta atgaataacg atgaacgcgc cgccgttggc
    26761 acctgctata aacagcaagg ctcagacgca gcgctggcgc tggggcataa actggtggcg
    26821 ggtgaaattc gtcagcagcg catggacgcc tggcgggcag cgtgcctgca aaatccgcaa
    26881 ggtattctct gctgcgcccg tggcggtcag cgctcacata ttgtgcaaag ctggttgtat
    26941 gcagcgggga ttgattatcc gctggtggaa ggcggttata aggcactgcg ccagaccgcg
    27001 attcaggcga ctattgaact ggcacaaaaa ccgatagtgc tgattggcgg ttgtactggc
    27061 agcggtaaaa cgctgttagt gcagcaacag ccgaacggtg ttgatctgga agggctggcg
    27121 cgccatcgcg gttcggcgtt tggtcgcacg ttacaaccac aacttagcca ggcgagtttt
    27181 gaaaacctgc tggctgccga aatgcagaaa accgacgccc gtcagaattt gcgcctgtgg
    27241 gtgctggaag acgaaagccg gatgatcggt tcgaatcacc tgccggaatg cctgcgcgag
    27301 cgaatgactc aggcggcgat tgcggtggta gaagatccgt ttgagatccg tcttgagcgc
    27361 ctgaacgaag agtatttctt gcgtatgcat catgatttta cccacgctta tggcgacgaa
    27421 cagggctggc aggaatattg cgaatacctg catcacggac tttcggcgat taagcgtcgg
    27481 ctggggttac agcgctataa cgaactcgct gcccagctgg atacggcgct gacaacgcaa
    27541 ctcaccaccg gcagcaccga cggtcatctg gcctggctgg tgccgttact taaagaatat
    27601 tacgacccaa tgtatcgcta tcagcttgag aaaaaagcgg aaaaagttgt ctttcgcggt
    27661 gagtgggcgg aagtggcgga atgggttaag gcgcggtaat gagggacgta tttgtattag
    27721 tcgcgacttg atctgacaca tggccttgaa aggttgagag ttaccggttt tgatatgagt
    27781 gtcgaatcct tatccaaaac aagaggtaac tctcatgctt catactgcca atcccgtcat
    27841 caaacacaaa gccggtttgc tcaatctggc tgaagaactc agcaacgtat caaaagcctg
    27901 taaaatcatg ggcgtttcgc gtgatacgtt ttatcgttac cgcgaactgg tcgctgaagg
    27961 cggtgtggat gcgcagatta atcgtagtcg ccgcgctcct aaccttaaga accgtaccga
    28021 tgaggcaact gaacaggctg ttgttgatta cgccgtcgct ttcccgacac atggtcagca
    28081 ccgggccagc aacgagctgc gtgaacaggg ggtttttatc tccgacagtg gtgtccgttc
    28141 tgtctggtta ctccataacc ttgagaatct caaaagacga tactgaacaa attttatcag
    28201 gtgacgttcc gtaaaaagtt gtatggagtt ctcgatgcag tcggcgcgac tatcagtacc
    28261 cccgctgaac cctggactat cgggccgccg tactggggca tgtcaccctg gcgtgccgct
    28321 ggttttccgc taatggttta tttcctcttt ctttcccggc aagaatttat tttaatccgg
    28381 aaggattatt tattttcaga tagggactca caataaacac gtaacatttt aattaatgca
    28441 tggctgaata tgtattacac aatgtcagat atcgtaaaag accagtgcaa ttctatgtaa
    28501 actctctgac tgttcatttt atttgttgtt tcagggtcgg aactcaccct caataaacaa
    28561 ctgaatacga gtagatattc atcgtctgag ctatatggct ttacacaata gccgacatgt
    28621 cttttctcag tcaggaattt tacttttcct cactacgaag ttgacgactt cgatatggga
    28681 tagactctta attcaagcaa ttactctgca caagcatcag gcaatccagc tccaatattt
    28741 gttataaaat aatgcgtaac attaatttca ccaacaaaaa aaaggtttac accatcccct
    28801 tgccctggta tcttaccata tttacaccac actttttttg tatctggatc attctttgat
    28861 acgattttcg ccacttgttc cctggccctc actgtcaatg ttttcttaaa cctaccccca
    28921 tcatcatttg ttagggaaaa ataagaaaaa aaataatcat cttctacatt tgtgtatatt
    28981 gatatatcca ccgctacgtg cggaaaaaaa aatctgggag ccatgtaaaa actccatcat
    29041 tttctggtat tgccatatag tttatgtaat gtttacttac aagagattca tctttgtata
    29101 aataaagata agtaattacg cataaaacaa caatgattat aatagcaaaa ataaatatta
    29161 tcatctttga tagattactt gagatagcca gcatcttgta aagcctttat cgttttttta
    29221 tgctctggat taatataatc actacatcta tctgagcaat ctgttgttga tggacatgtc
    29281 aacccatggt catttacagc caagtctttt ttattatctt caatcatttc agaatgggat
    29341 agctttactt ttctgccgta cataccgaac agatttaacc cgtaatctct aatctctttt
    29401 tcataaccca gccctttcgc cgatcggcta acaccagcgt catttaattt agacactcga
    29461 caaaatgcca tacaatggaa aaattgatca gtcccaccta aatttaatct cttcatatct
    29521 tcataagtat cagataatat atcccatgct ctttgatggt ttagttgatc ttttcttctt
    29581 attagcgcta catctgcggg acttaaccct aatggatcaa taccattcac cggattcagc
    29641 gggtacgcat acagactcca tccccccctc cagccctatc gggtcctgag tgatataccg
    29701 cccctgcaac ggatcgtagt accggttccg gttgtagtac agccccgact ccttatcata
    29761 ctgctgcccc ggcagccggt acggctggtg caggtgatgc gggttctctt cattcagctg
    29821 gttgccccac tcatcatact ccccgctcca cgcaatgttt ccatccgcat ccatcagcgc
    29881 cagcattatt tactatgtag gaaattcaaa acacatccat ttttcttatt aatttctata
    29941 ataaatacac cacccgcaac ttcataaggt aactttgctc cctcaacaac ccaactatct
    30001 ggtagttccg taattaaata tggtttttct tcttctgcaa tccgttcgcc atagcgatac
    30061 ctaacatata tttcagctaa ttgtatcgcc attgtttcag tttttaccgt atttttaaca
    30121 gagtatttat ctaaatgtaa tttttccaca tacctcataa aaataatgtt ttcatcagaa
    30181 ccgtaaacag ctgtctcatt acaggcaaat agtataaaaa atgaaagcat cgaaaatatt
    30241 gaactatatt tcattgaatc tccttatagg ccccactaat aaaagcacta aaattaatca
    30301 tgatacggta tgcatactga actcaatccc gggacactat ttctgataaa aatatattct
    30361 cctttattat taagcgcctc aaatcgtcca tcaggtgttg cgagataaaa tgcttcaaga
    30421 ttattatctt tacttcttac aagattttta tcgctacttg aaaaaaattc atcaacataa
    30481 tcgccatgac tatctgcacc gtgagtatga taagcagcaa ctctatctgt acctgtggga
    30541 cattttcttt tcagaggata tgactccttt cttaaattat cagtttctgc cttagatgca
    30601 aaatattttc cattagtatc tttacagaca ataccagagt attccatatc attacataga
    30661 gatctattct gcgtttcttt caaagcatct aacgcagcat ctgcagtact atcaaattta
    30721 cttggtccta ttatacgtga aagaacacca caaactcccc ccgtacatgc tccagatctg
    30781 gcatcatccc aagtctgcaa taatcccata gggtcaattt gttgtagtgg atttaaagga
    30841 tactgatata aattccatcc ccctttcaac cccatcgggt cctgagtaat ataccgcccc
    30901 tgcaacggat cgtagtaccg gtgacggtta tagtacagcc ctgattcctc atcatgctgc
    30961 tgccctggca gacggtacgg ctgatacaca tgatgcgggt tctcctcatt aagctggttg
    31021 ccccattcat catattccgc gctccacgcc gtattgccgt cttcgctgat aagcgccagc
    31081 ggcagtcccc ggtggtcgca gtgataaaga tgagcttttc gcgccggtgt gtattccggc
    31141 tccacctgtc tggccagttg ctccaccgtc agcccgcact gcgcaagcca cgcccggctt
    31201 tcactgctca cgcggtctgc ccggatttct tcctccagtc tgtccagcag ccgcaccagt
    31261 tcagccggaa ataccacgcc gtgcccgtcc tcgctccctt cctgctgcag cttctccgcc
    31321 aggctgcggc gctgcgcttt ctcccgctcg ccgttgtctg tttcgatgcg gatgagcggc
    31381 gcgaagctcc ccggctg
//
LOCUS       DAZFFI010000050        31359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFFI010000050 DAZFFI010000000
VERSION     DAZFFI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.050"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..588
                     /locus_tag="ACHJ12_002632"
     CDS             <1..588
                     /locus_tag="ACHJ12_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4990397.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACHJ12_002633"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACHJ12_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4990398.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACHJ12_002634"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACHJ12_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4990399.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACHJ12_002635"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACHJ12_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4990400.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACHJ12_002636"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACHJ12_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4990401.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHJ12_002637"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACHJ12_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4990402.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACHJ12_002638"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACHJ12_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4990403.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHJ12_002639"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACHJ12_002639"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4990404.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHJ12_002640"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACHJ12_002640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4990405.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13246..13785
                     /gene="yjaZ"
                     /locus_tag="ACHJ12_002641"
     CDS             13246..13785
                     /gene="yjaZ"
                     /locus_tag="ACHJ12_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO4990406.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14195..15328)
                     /gene="thiH"
                     /locus_tag="ACHJ12_002642"
     CDS             complement(14195..15328)
                     /gene="thiH"
                     /locus_tag="ACHJ12_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4990407.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15325..16095)
                     /gene="thiG"
                     /locus_tag="ACHJ12_002643"
     CDS             complement(15325..16095)
                     /gene="thiG"
                     /locus_tag="ACHJ12_002643"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4990408.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16097..16297)
                     /gene="thiS"
                     /locus_tag="ACHJ12_002644"
     CDS             complement(16097..16297)
                     /gene="thiS"
                     /locus_tag="ACHJ12_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4990409.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16281..17036)
                     /gene="thiF"
                     /locus_tag="ACHJ12_002645"
     CDS             complement(16281..17036)
                     /gene="thiF"
                     /locus_tag="ACHJ12_002645"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4990410.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17029..17664)
                     /gene="thiE"
                     /locus_tag="ACHJ12_002646"
     CDS             complement(17029..17664)
                     /gene="thiE"
                     /locus_tag="ACHJ12_002646"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4990411.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEVDVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17664..19559)
                     /gene="thiC"
                     /locus_tag="ACHJ12_002647"
     CDS             complement(17664..19559)
                     /gene="thiC"
                     /locus_tag="ACHJ12_002647"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4990412.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19640..19740)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19792..20268)
                     /gene="rsd"
                     /locus_tag="ACHJ12_002648"
     CDS             complement(19792..20268)
                     /gene="rsd"
                     /locus_tag="ACHJ12_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4990413.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20363..21136
                     /gene="nudC"
                     /locus_tag="ACHJ12_002649"
     CDS             20363..21136
                     /gene="nudC"
                     /locus_tag="ACHJ12_002649"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4990414.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21176..22240
                     /gene="hemE"
                     /locus_tag="ACHJ12_002650"
     CDS             21176..22240
                     /gene="hemE"
                     /locus_tag="ACHJ12_002650"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4990415.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22250..22921
                     /gene="nfi"
                     /locus_tag="ACHJ12_002651"
     CDS             22250..22921
                     /gene="nfi"
                     /locus_tag="ACHJ12_002651"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4990416.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPVMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22964..23554
                     /gene="yjaG"
                     /locus_tag="ACHJ12_002652"
     CDS             22964..23554
                     /gene="yjaG"
                     /locus_tag="ACHJ12_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4990417.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23741..24013
                     /gene="hupA"
                     /locus_tag="ACHJ12_002653"
     CDS             23741..24013
                     /gene="hupA"
                     /locus_tag="ACHJ12_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4990418.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24026..24721
                     /gene="yjaH"
                     /locus_tag="ACHJ12_002654"
     CDS             24026..24721
                     /gene="yjaH"
                     /locus_tag="ACHJ12_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4990419.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24723..25154)
                     /gene="zraP"
                     /locus_tag="ACHJ12_002655"
     CDS             complement(24723..25154)
                     /gene="zraP"
                     /locus_tag="ACHJ12_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4990420.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGMGYGGCGGGGHMGMGHW"
     gene            25392..26768
                     /gene="zraS"
                     /locus_tag="ACHJ12_002656"
     CDS             25392..26768
                     /gene="zraS"
                     /locus_tag="ACHJ12_002656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4990421.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26765..28090
                     /gene="zraR"
                     /locus_tag="ACHJ12_002657"
     CDS             26765..28090
                     /gene="zraR"
                     /locus_tag="ACHJ12_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4990422.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28087..29376)
                     /gene="purD"
                     /locus_tag="ACHJ12_002658"
     CDS             complement(28087..29376)
                     /gene="purD"
                     /locus_tag="ACHJ12_002658"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4990423.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAETQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29388..30977)
                     /gene="purH"
                     /locus_tag="ACHJ12_002659"
     CDS             complement(29388..30977)
                     /gene="purH"
                     /locus_tag="ACHJ12_002659"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4990424.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7405 a         8113 c         8581 g         7260 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggt cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ctaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc actgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgta tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgtt ctgggtctgt actatatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgcaaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaatactg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggtca
    11941 gaccattact cgtcagacag acgaattgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggggcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc tgaatactca
    13141 taatcaatgt agagatgact aatatcctga aactgactga actaattgag tcaaactcgg
    13201 caaggattcg atactattcc tgtgtaactt tcttaaggaa cgagaatgaa acaggaagtg
    13261 gaaaagtggc gaccttttgg acatccggat ggtgatattc gtgatttatc atttcttgat
    13321 gctcatcagg ctgtctacgt tcagcatcat gagggcaaag agcctttaga gtatcgcttt
    13381 tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac gaacgaagaa
    13441 aaacaatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca gcaccgttat
    13501 aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag caacgttatt
    13561 catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg agataaggca
    13621 ttttactttg ttgcgttcag ggctttcagg gaaaagaaaa aactccgttt gcatgtaact
    13681 agcgcttatc ccatttctga aaaacagaaa ggtaaatcag tgaaattttt caccattgcc
    13741 tacaacttat tgagaaataa gcagcttcct cagccctcaa aataacaaaa cccaccttaa
    13801 ggtgggtttc gccagagaat tatctctggt attcagaacg ccattaccgg actttgcctt
    13861 gaccttgcga taatcgcagg ttgcgggatg tctgaatttc ttcagtctgc tgcatcctgg
    13921 aagatgagaa catgtgttct tattttcgtc tctatcatag ttgagtattt actctcttac
    13981 aatcagatct ctttcattgc tcaacaggcg atggcttcag actttgcatt acggaatttt
    14041 taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac
    14101 ggcactacca taaaaaaaca gaacgctttc aacaaccttg cgtttttcat cttccgtagc
    14161 ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag gcccgtccca
    14221 gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac gcggcagcaa
    14281 ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg tgattatcgg
    14341 catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc agcggaataa
    14401 cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa
    14461 gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc gacgcaggct
    14521 caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt
    14581 aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg cgccagttgt
    14641 cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc cccagccgat
    14701 ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcagatga tggcgggcat
    14761 aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc atccagacca agctgcttta
    14821 actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa gagaactgtt
    14881 cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac
    14941 cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt tccctggcaa
    15001 tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat ccgcagtacg
    15061 tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta actgtgttgc
    15121 caaatcgctg acgggtcaga cgctgcgccc gttgggccag ttgttccaga tagccactgg
    15181 cggctggcga taacagcgcc atcatatcgt cgcgggtgaa ttgcgaggca tttagcgccc
    15241 gctctacgtc aacagccgtt ttgccgttga tacgcaggcg gatgtcatcc cagtccagtt
    15301 gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag tcagcgggct
    15361 ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc
    15421 ttcaaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta cggcaatcgc
    15481 cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat ggctgggaac
    15541 gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa tctccagcat
    15601 ggcacaggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc
    15661 acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac
    15721 gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa
    15781 cagccagcgg gcgtcagggt gaatctccaa ttttagccag ttagtgccta acgcttcacg
    15841 tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggatg tatttggcag
    15901 cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc
    15961 gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg cctccaccat
    16021 cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc
    16081 cgcaatacgt aacatttcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat
    16141 cctgcacgat atgttgcgcc cactgctcac gcgggacgat ttgctgatta atcgccagag
    16201 ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg
    16261 cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa
    16321 ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc gaacagtcgg
    16381 agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag tgcctgcaaa
    16441 gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc
    16501 tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg cgtcagtacc
    16561 atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg
    16621 caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg
    16681 gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa tgctgtcagt
    16741 tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt cgggcgatcg
    16801 atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca
    16861 tctgccagta ccagcgtccc gacgccagcg cccgccaggt acagcgcagc aggtgtaccc
    16921 agcccgccca gaccgataat cagcacctgg ctgtcgagca gtttttgctg cccgtccaga
    16981 gcgatatcgt cgagcaggat ttggcggcta taacgcataa agtcacggtc attcatcgcc
    17041 aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcag cttgagtaat
    17101 ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgtgccag
    17161 actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat gccgtgccag
    17221 ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc
    17281 cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt
    17341 cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc
    17401 ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc agtaatcgtt
    17461 gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga catcgacttc
    17521 cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa
    17581 cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac gaaaaggtac
    17641 aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat ttctccgcct
    17701 ctggcacgga agttctccga catatccgcc attcccattt caatagtttg cgtggcggcg
    17761 taatcacgca cttcctggct gattttcatc gagcaaaatt tcggtccaca catggagcaa
    17821 aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc gcgggcggta
    17881 aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac
    17941 atggcgttat cgcgaatttg cgcgcccgga tgccctttcg ccaggtcagc ggcgtgggcg
    18001 gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag acccagatgc
    18061 tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat cgccgcacca
    18121 atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg ccccagagtg
    18181 taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg gatcatctgc
    18241 atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca ggcaattttg
    18301 gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc gtcctgaata
    18361 gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc acagatttca
    18421 caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc
    18481 gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcaca
    18541 tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc
    18601 agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt aaccttctcc
    18661 agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg
    18721 cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc tccccagcgc
    18781 gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc cgagttgccg
    18841 atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc cgattccgga
    18901 tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc
    18961 gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa
    19021 acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa ttccatttcc
    19081 ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc tgctttggcg
    19141 cgttttggtg ttagtacgcc gctaaaacgc agttcgtcga ggccatcatc tgccagccgc
    19201 gctttagtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc
    19261 cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcaatctg cggatcacca
    19321 tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct
    19381 ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac gcgcacgccg
    19441 gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg
    19501 tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata
    19561 gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc
    19621 gccgcagggg cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat
    19681 caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt
    19741 agccccgcaa atggggcatt gaatgtaaat tacgcgttaa cagcgcagaa ctcaagcagg
    19801 atgtttgacg cgggcggcgt caagcaccag cagaatcagc ttatcttcca gcacaaagcg
    19861 cgcttccagc gcctcgccga tgtcagataa aacctgttga aactcaaggt aattatcatg
    19921 atcgatggcg gtttccagac tggaatcgta gtaatccata atctgttgag tattggcttc
    19981 gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg ttgccttcca gcttatgaag
    20041 aatgcgctca taaatactga aatgtccggc agacaagtaa tcgaccaggc tctgacaaaa
    20101 atcatcaagg gctttttcgt ttagcctcat gtacgattct ttgccaggct taatgccaac
    20161 cagattgtag taagccacga gcagatgctt acgtacatgt agccagcgat caaccagttt
    20221 gttacttcct ctgacgcgct ccgtcaggtt atcgagctgg ttaagcatga ttgactccgc
    20281 aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa ccatgttaaa catgccagtg
    20341 atgcaaaggt agtgcaagag ctatggatcg tataattgaa aaattagatc acggctggtg
    20401 ggtcgtcagc catgaacaaa aattatggtt gccgaaggga gaattgccat atggcgaagc
    20461 ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc ggcgaatggc agggggaacc
    20521 tgtttggtta gtacaacagc agcggcgtca cgatatgggg tcggtacgtc aggtcattga
    20581 tctcgatgtt gggctgtttc aactggccgg acgaggcgta caactggcgg agttttaccg
    20641 atcgcataaa tactgtggtt actgcgggca tgaaatgtat ccgagcaaaa ccgaatgggc
    20701 gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa atcgccccct gcattattgt
    20761 tgccatccgt cgcgatgatt cgatcctcct cgcccagcat acccgccatc gtaacggtgt
    20821 ccatacagta cttgccggat tcgtcgaagt gggcgaaacc ctcgagcagg cagtcgcgcg
    20881 ggaagtgatg gaagagagcg gaattaaagt taaaaacttg cgttacgtga cttctcagcc
    20941 gtggccgttt cctcagtctt taatgaccgc gtttatggcg gaatatgaca gcggcgacat
    21001 cgtgatcgac ccgaaagaat tgctcgaggc gaactggtat cgctatgacg atttgccgtt
    21061 actcccgccg cccggcaccg tagcgcgccg tctgatagaa gatacggtgg cgatgtgtcg
    21121 ggcagagtat gagtgatgat acactgaccg cctgacgcac taaggaacag ccaaaatgac
    21181 cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc cagcccgttg atgtcactcc
    21241 agtatggatg atgcgccagg cgggtcgcta tctaccggaa tataaagcca cgcgcgccca
    21301 ggcgggcgat tttatgtcgc tgtgcaaaaa cgccgagctg gcgtgcgaag tgactttgca
    21361 accgctgcgt cgctacccgc tggatgcggc gatcctcttt tccgatatcc tcaccgtgcc
    21421 ggacgcgatg gggttagggc tctattttga agccggagaa ggtccgcgtt ttacctcgcc
    21481 agtcacctgc aaagccgacg tcgataaact gccaattccg gacccggaag atgaactggg
    21541 ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg aaaggcgaag tgccgctgat
    21601 tggtttttcc ggcagcccgt ggacgctggc gacctacatg gtggaaggcg gcagcagcaa
    21661 agccttcacc gtgatcaaaa aaatgatgta tgccgatccg caggcgctgc acgctctgct
    21721 cgataaactg gcgaaaagcg tcactttgta tctgaatgcg cagattaaag ccggtgctca
    21781 ggcagtgatg attttcgaca cctggggcgg cgtgcttacc gggcgcgatt atcaacagtt
    21841 ctcgctctat tacatgcata aaattgttga tggtttagtg cgtgaaaacg acggtcgccg
    21901 cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg ctggaagcca tggcagaaac
    21961 cggttgcgat gcgctgggcc tcgactggac aacggacatc gccgatgcgc gccgccgtgt
    22021 gggcaataaa gtcgcgttgc agggtaatat ggatccgtcg atgctgtacg cgccgcctgc
    22081 ccgcattgaa gaagaagtag cgactatact tgcaggtttc ggtcacggcg aaggtcatgt
    22141 ctttaacctt ggtcacggca ttcatcagga tgtgccgcca gaacatgctg gcgtgttcgt
    22201 ggaggcagtg catcgactgt ctgaacagta tcaccgctaa ggagtgatta tggatctcgc
    22261 gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg atccgcgagg atcgactcga
    22321 taaagatcca ccggatctga tcgccggagc cgatgtcggg tttgagcagg gcggagaagt
    22381 gacgcgagcg gcgatggtgc tgctgaaata tccctcgctt gagctggtgg agtataaagt
    22441 tgcccgcatc gccaccacca tgccttacat tccaggtttt ctttccttcc gcgaatatcc
    22501 tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg
    22561 tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc cattttggct tattggtgga
    22621 tgtgccgacc attggcgtgg cgaaaaaacg gctctgcggt aaattcgaac cgctctccag
    22681 cgaaccgggc gcgctggccc cagtgatgga taaaggcgag cagctggcct gggtctggcg
    22741 cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag
    22801 cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg
    22861 ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta
    22921 attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat
    22981 tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc actttcatgg cttgcttatg
    23041 cgaacgcatg taccccaatt acgccatgtt ctgccagcaa accggttttg gtgatgggca
    23101 aatttaccgt cgcattctcg atctcatctg ggaaacgctg accgttaaag acgcaaaagt
    23161 aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga
    23221 tctgtacggc gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc
    23281 gcgtttgagt ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac
    23341 cgttgcgatg ctggaaatga ctcaggctgg tcgcgaaatg agcgatgaag agcttaaaga
    23401 aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga
    23461 gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgaggcggg
    23521 tgatagcaat attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg
    23581 atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa
    23641 aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact
    23701 cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat
    23761 tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc
    23821 cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt
    23881 cggtaccttc aaagtgaacc accgcgctga gcgtactggc cgcaacccgc agaccggtaa
    23941 agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga
    24001 cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct
    24061 tttgtctttc tggcgtcgat cattgatgct ggcaggcgct ctgtttctca ctgcctgtag
    24121 tcacaactct tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt
    24181 acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg
    24241 gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc tcacgctcat
    24301 caatatcaat gtttacagca aaccgccggt gaacattcgc gcgcgttttg acgatcgcgg
    24361 tgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca
    24421 aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca
    24481 ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg
    24541 cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg
    24601 ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca
    24661 attactgtta gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg
    24721 atttaccagt ggcccatacc catatgaccg ccaccaccgc agccgccgta gcccatgccc
    24781 attcccattc cggctccgcg cggaataccc gcttcagcca tcgcgatatc tcgtttcacc
    24841 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    24901 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    24961 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    25021 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    25081 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    25141 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    25201 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25261 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25321 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25381 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25441 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25501 attatgggcg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25561 ttcgcgctct tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25621 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    25681 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    25741 ccccgcagga aatgcagcag ttacatcctg gagatgaaga agcgtggcgg cggatcgata
    25801 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    25861 gaatgcaccg gatgcgccat atgcagcagt atgccgcaac accacaagca attttcatcg
    25921 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    25981 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    26041 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    26101 cgctggggca tcttgcggca ggcgttgccc acgaaatccg taatccactt tcctcaatta
    26161 aagggctggc gaaatacttt gccgaacgcg cgccagcagg gggagaagcg catcaattgg
    26221 cgcaggtgat ggcgaaagaa gccgaccgtt taaaccgcgt ggtaagcgag ttgctggaac
    26281 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26341 cattacagct ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca
    26401 acgacacatt accggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26461 tctatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggtcagcg
    26521 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26581 agcttgaagc catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc
    26641 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg
    26701 agggaaaagg ctcaacgttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    26761 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    26821 tattttgcag gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    26881 acaggcgctg gagcaggtgc gggaacaggt ttttgatctt gtgctttgcg atgtgcgaat
    26941 ggcggagatg gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc cggcaattcc
    27001 ggtgctgatt atgactgcgt actccagcgt cgagacggcg gtagaggcac tgaaaactgg
    27061 ggcgctggat tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa
    27121 ggcgctcgcg catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt
    27181 cggtatggtc ggtaaaagct cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27241 gccatcggaa gccacggtac tgatccacgg cgattccggc accggtaaag agctggtcgc
    27301 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27361 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27421 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct
    27481 cgatgaaatt ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga
    27541 gcgcgaagtt cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc
    27601 ggcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    27661 ctatcgcctg aatgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat
    27721 ccctctgctg gctggccatt ttctgcagcg ctttgccgag cgtaatcgca aggcggtaaa
    27781 aggttttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    27841 tgagctggaa aacgcggtgg aacgggcagt ggtgctgctg accggggaat atatttccga
    27901 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggggcaaa gtcaggatat
    27961 tcagccgctg gtggaagtgg aaaaagaggt gattctggcg gcactggaga aaacgggcgg
    28021 caacaaaact gaagccgccc gtcagttagg gatcacgcgt aaaacgttat tggcaaaact
    28081 gtcgcgttag ttctgctcgc gttcgatagc acgccagccg atatctttcc ggcagaagca
    28141 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgcgttt ctgccacggt
    28201 atgacccagc gcggtgacgc acagtacgcg tccgccgttg gttaccacct gctcgtcatc
    28261 cgccagtttt gtgcccgcgt ggaacacttt gccgtcagcc acttcttcca gcggcaggcc
    28321 gtggatcaca tcgccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28381 gagagaagcg cgttcatccc actcggacgt tttctcgtcc agcttgcctt cacaggccgc
    28441 caggcagagc tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc
    28501 gccaaagcgg cagttaaatt caataacctt cggattgccc tgtttgtcga tcatcaggcc
    28561 cgcatagaga aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca
    28621 gataatacgt tccatggtgc gctgatgaac gtcatcggtt actaccggcg cgggggagta
    28681 agcgcccatc ccgccggtgt ttggtccggt atctttatcg cctacgcgtt tgtgatcctg
    28741 gctggtagcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    28801 gccatcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc
    28861 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    28921 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    28981 cagcgcaggt tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc
    29041 caggaaatct ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc
    29101 gaagattttc agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    29161 cgggccgacg atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat
    29221 atcggtcacg ccaatagcaa cgttttgcag cgcgggttcc agtgcagtgc ctgcattacc
    29281 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29341 ctcgcgcccg ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag
    29401 tggcgcatgt cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29461 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29521 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29581 aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    29641 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    29701 aacttcgcca ctttccagca gaacagcgca tcgcgcagtt cctgttcggt cggctgacgt
    29761 tgggtgacga cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc
    29821 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    29881 gtcagaacgc gtacgttctg cttggcggcg gtgattttca gggcttcttc gctggcggaa
    29941 ggcgcaataa tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatct
    30001 agctcgcggt taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg
    30061 cgatcgtaag catcaagaat ggaattgccg atagctacac cgcaagggtt ggcgtgcttc
    30121 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    30181 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30241 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30301 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct
    30361 ttgctttcac cgtggtacgc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30421 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30481 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30541 attgcgacat ctttatggtt cttagcggcg gagcgcacca tggtcgggcc gccgatatcg
    30601 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    30661 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    30721 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    30781 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    30841 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    30901 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    30961 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    31021 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    31081 tgtaacgaaa acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt
    31141 ttgacagtgg ttaccgtggg aaaaatacag aaattacatt gatattgtgg ataactctgt
    31201 gcgtaaaaag gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc
    31261 aatttttcta ttgcggcctg cggagaactc cctataatgc gcctccatcg acacggcgga
    31321 tgtgaatcac ttcacacaaa cagccggttc ggttgaaga
//
LOCUS       DAZFFI010000051        30425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFFI010000051 DAZFFI010000000
VERSION     DAZFFI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.051"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..1665
                     /gene="rtcR"
                     /locus_tag="ACHJ12_002660"
     CDS             67..1665
                     /gene="rtcR"
                     /locus_tag="ACHJ12_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4990425.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1647..2405)
                     /locus_tag="ACHJ12_002661"
     CDS             complement(1647..2405)
                     /locus_tag="ACHJ12_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4990426.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2422..3252)
                     /gene="glpG"
                     /locus_tag="ACHJ12_002662"
     CDS             complement(2422..3252)
                     /gene="glpG"
                     /locus_tag="ACHJ12_002662"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4990427.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3297..3623)
                     /gene="glpE"
                     /locus_tag="ACHJ12_002663"
     CDS             complement(3297..3623)
                     /gene="glpE"
                     /locus_tag="ACHJ12_002663"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4990428.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3813..5318
                     /gene="glpD"
                     /locus_tag="ACHJ12_002664"
     CDS             3813..5318
                     /gene="glpD"
                     /locus_tag="ACHJ12_002664"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4990429.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5372..5977)
                     /locus_tag="ACHJ12_002665"
     CDS             complement(5372..5977)
                     /locus_tag="ACHJ12_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990430.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5980..6747)
                     /locus_tag="ACHJ12_002666"
     CDS             complement(5980..6747)
                     /locus_tag="ACHJ12_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990431.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(6750..7460)
                     /locus_tag="ACHJ12_002667"
     CDS             complement(6750..7460)
                     /locus_tag="ACHJ12_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990432.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(7475..8983)
                     /locus_tag="ACHJ12_002668"
     CDS             complement(7475..8983)
                     /locus_tag="ACHJ12_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4990433.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(9108..11555)
                     /gene="glgP"
                     /locus_tag="ACHJ12_002669"
     CDS             complement(9108..11555)
                     /gene="glgP"
                     /locus_tag="ACHJ12_002669"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4990434.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11574..13007)
                     /gene="glgA"
                     /locus_tag="ACHJ12_002670"
     CDS             complement(11574..13007)
                     /gene="glgA"
                     /locus_tag="ACHJ12_002670"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4990435.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13007..14302)
                     /gene="glgC"
                     /locus_tag="ACHJ12_002671"
     CDS             complement(13007..14302)
                     /gene="glgC"
                     /locus_tag="ACHJ12_002671"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4990436.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14320..16293)
                     /gene="glgX"
                     /locus_tag="ACHJ12_002672"
     CDS             complement(14320..16293)
                     /gene="glgX"
                     /locus_tag="ACHJ12_002672"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4990437.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16290..18476)
                     /gene="glgB"
                     /locus_tag="ACHJ12_002673"
     CDS             complement(16290..18476)
                     /gene="glgB"
                     /locus_tag="ACHJ12_002673"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4990438.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQFYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18749..19852)
                     /gene="asd"
                     /locus_tag="ACHJ12_002674"
     CDS             complement(18749..19852)
                     /gene="asd"
                     /locus_tag="ACHJ12_002674"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4990439.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCRGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHJ12_002675"
     CDS             20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHJ12_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4990440.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20684..21979)
                     /locus_tag="ACHJ12_002676"
     CDS             complement(20684..21979)
                     /locus_tag="ACHJ12_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO4990441.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQLELFSLHEDDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(21886..23982)
                     /locus_tag="ACHJ12_002677"
     CDS             complement(21886..23982)
                     /locus_tag="ACHJ12_002677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO4990442.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGRCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(24122..25462)
                     /gene="gntU"
                     /locus_tag="ACHJ12_002678"
     CDS             complement(24122..25462)
                     /gene="gntU"
                     /locus_tag="ACHJ12_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4990443.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(25466..25993)
                     /gene="gntK"
                     /locus_tag="ACHJ12_002679"
     CDS             complement(25466..25993)
                     /gene="gntK"
                     /locus_tag="ACHJ12_002679"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4990444.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(26132..27127)
                     /gene="gntR"
                     /locus_tag="ACHJ12_002680"
     CDS             complement(26132..27127)
                     /gene="gntR"
                     /locus_tag="ACHJ12_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4990445.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(27351..28046)
                     /gene="yhhW"
                     /locus_tag="ACHJ12_002681"
     CDS             complement(27351..28046)
                     /gene="yhhW"
                     /locus_tag="ACHJ12_002681"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4990446.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(28169..29206)
                     /gene="yhhX"
                     /locus_tag="ACHJ12_002682"
     CDS             complement(28169..29206)
                     /gene="yhhX"
                     /locus_tag="ACHJ12_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4990447.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            29538..30026
                     /gene="yhhY"
                     /locus_tag="ACHJ12_002683"
     CDS             29538..30026
                     /gene="yhhY"
                     /locus_tag="ACHJ12_002683"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4990448.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            30263..>30425
                     /locus_tag="ACHJ12_002684"
     CDS             30263..>30425
                     /locus_tag="ACHJ12_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO4990449.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLG"
BASE COUNT         7655 a         8126 c         7468 g         7176 t
ORIGIN      
        1 aacggagatt ttccacaaaa ccaattcacc gataatgaaa tatcgtcttt tataaggata
       61 tctaagatgc gtaaaacagt ggcttttggc tttgtcggta ccgtgctgga ttatgccgga
      121 cgcggcagtc agcgctggtc gaaatggcgt ccgacgcttt gtttatgcca gcaagaatcg
      181 ttagtcatcg atcggctgga attgttgcac gacacccgct cgcgctcgct attcgaaacg
      241 cttaaacgcg atatcgccag cgtttcgcca gaaacagaag tcgtgggcgt tgagattgaa
      301 ctgcataacc cgtgggattt cgaagaggtt tacgcctgcc tgcatgattt cgcccgtggt
      361 tacgagtttc agccagaaaa agaagactat ttaattcaca tcaccaccgg cacccacgtc
      421 gcgcagattt gctggtttct gctggcggaa gcgcgttacc tgcccgcccg gctgatacaa
      481 tcttcacctc cgcgcaaaaa agaacagccg cgcggcgcag gtgaagtgac gattatcgat
      541 ctcgatttaa gccgttataa cgccatcgcc agccgctttg ccgaggaacg ccagcaaacg
      601 cttgattttc ttaagtccgg cattgccacg cgtaaccccc acttcaaccg catgattgag
      661 cagatcgaaa aagtggcgat caaatcccgc gcgccgattc tgcttaacgg tccaaccggc
      721 gcgggcaagt catttctggc gcgacgcatc ttcgagttaa aacaggcgcg gcatcagttt
      781 agcggcgcgt ttgtggaagt gaactgcgcc accctgcgcg gcgataccgc catgtcgacg
      841 ctgtttggtc atgtaaaagg cgcgtttacc ggggcgcggg aatctcgtga aggtttatta
      901 cgcagcgcca acggcggaat gttgtttctt gatgagattg gcgaactggg cgcagacgaa
      961 caagcaatgc tgctaaaagc cattgaagag aaaacctttt acccgtttgg cagcgatcgc
     1021 caggtgagca gtgattttca gcttatcgcc ggaacggtgc gcgatttgcg ccagctggtt
     1081 gccgaaggca aatttcgcga agatctgtac gcgcggatca atctctggac cttcaccctg
     1141 ccgggtctac gccagcgcca ggaagatatt gaaccgaacc ttgattatga agtggagcgc
     1201 cacgccacac tcactggcga cagcgtgcgt tttaacaccg aagcgcggcg cgcctggttg
     1261 gcttttgcga cctctcccca ggcaacatgg cgcggtaact ttcgcgaact ttctgccagc
     1321 gtcacgcgga tggccacctt tgccactagc ggacgcatca ctctggacgt ggttgaagat
     1381 gagataaacc gtctgcgcta taactggcag gagagccgcc cctccgcgct tacggcgttg
     1441 ctgggcgcag aggcagaaaa catcgatctc ttcgaccgta tgcaactgga acacgttatc
     1501 gctatctgcc gccaggcaaa gtcgctttct gcagccggac gacagctttt tgatgtttcg
     1561 cgccagggca aagccagcgt caatgacgcg gatcggctac gcaaatacct ggcgcgtttt
     1621 ggtctgacgt gggaagccgt gcaggatcag cacagctcca gttgaatatg atggtccgtc
     1681 agcacctgca ttacgcttac tggcggcggg gcgtcggtgt agacggcatc taccatgctg
     1741 atgctgccca tattgaccat cgcgttacgg ccaaatttcg agtgatcgac aaccagcata
     1801 acgtggcgcg agttctcaat aatggcgcgc ttggtgcgaa cttcgtggta atcgaactcc
     1861 agcagcgagc cgtcgctatc gatgccgctt atccccagaa tgccgaaatc aaggcggaac
     1921 tgggagataa aatcgagcgt cgcttcgcca atgatcccgc catcgcggct gcgtaattcg
     1981 ccaccggcga gaataatgcg gaaatcttct tttaccatca acgtgttagc aacattgaga
     2041 ttgttggtga caatacgcaa attgctgtga ttaagcagcg cgtgcgccac cgcttccggc
     2101 gtggtgccga tatcgataaa cagtgtcgag ccattgggga tttgctccgc cactttgcgg
     2161 gcaatcctct ctttttcttc ggtctgggtg gctttacgat cgtgccacgg cgtgttaacc
     2221 gaactggaag gcagcgccgc accgccatgg tgacgcagga tcaggttttg ctccgccagc
     2281 tcattgaggt cgcggcgaat agtctgcggg ctgacggaga aatgctctac cagctcttca
     2341 gtactgacat aaccctgctg tttaaccagt tcgataatac cgttgtgacg ttgtgtttgt
     2401 ttcatttata aatccctgga attattttcg ttttcgcgca ttgagcgaat caacaaaagc
     2461 catcgctaaa cccacggcta acccggcgat gtgtgctccg ttcgccatcg acatcccaaa
     2521 caaatcaaac catccggcga caatccagat cagcgcaaag ataattaacc cacgttgcag
     2581 gtaaatgcca ctttgcggat cgcgttcgcc acgtagccag acgtagccca tcagcgcata
     2641 caccacgcca gaaagcccgc caaaccacgg cccgctgaat ttttgctgca catagccgct
     2701 taacagggcg ctaatgagag taataacaat tagcttaccg ctaccgaggc gtttttccac
     2761 cgcaccgccg agataccacc accagagcag gttaaagagg atatgcatca gcgagaagtg
     2821 cattaacgcg tgggtgaagt aacgccagaa ctcaaatttc agtgttggat cgaatggcca
     2881 ggccagccat aacatcactt cctgatcgcc gagaatttgc atggcgataa acaccaccac
     2941 gcaggcgatc atcaccaccc aggttaccgg acctgcgcgt tcacgcaagg cagcaaagaa
     3001 aggataacgg cgataatgca gaccactgcc ggtatggccc gcctgccagc tggccgccag
     3061 ataacgcgga tctgccgggt tttcgagaaa acgcgccagc tccgcccgca cgcgctcggc
     3121 ctgggactca tccgccagcc agacatcgct ttggttatgt tgttgaatcg tgaggataac
     3181 accctgcgtc gccatataat caacaaacgc ctgcgccacg cgggggttag caaaagaggt
     3241 aatcatcaac atcgttgctg tcgcttattc cacacaaaag gggacagtat aaagcgttac
     3301 gcgccgtacg ccacctctgc gggaaactga cgctgccagg cttcaaaacc gccgtcaatg
     3361 ctatagacca catcgtagcc ctgttgcagc agatactgcg ccgcgccttt gctgctattg
     3421 ccgtgataac acatcaccat caccggagtg tcaaagtcgt tatcacgcat aaacgcgccc
     3481 agcgtgtcgt tggttaaatg gaaagcctgc accgcatgcc ccatagcgaa actctgtgga
     3541 tcgcgaatat cgaccagcac cgcctctttt tcctgcaact tctggtgcgc gtcggcaacg
     3601 ttaatacatt cgaactgatc catgcgtctc tctttcttta caaacaagtg ggcaaattta
     3661 ccgcacagtt tacgtcgaag cggcagataa acgccataat gttatacata tcactctaaa
     3721 atgttttttc aatgttacct aaagcgcgat tctttgctaa tatgttcgat aacgaacatt
     3781 tatgagcttt aacgaaagtg aatgagggca gcatggaaac caaagatctg attgtgatag
     3841 gtggcggcat caatggtgct ggtatcgcgg cagacgccgc tggacgcggt ttatccgtgc
     3901 tgatgctgga ggcgcaggat ctcgcttgcg cgacctcttc cgccagttca aaactcattc
     3961 acggtggcct gcgctacctt gagcactatg aattccgcct ggtcagcgag gcgctggctg
     4021 aacgtgaagt gctgctgaaa atggctccgc atatcgcctt cccgatgcgt tttcgcctgc
     4081 cacatcgtcc gcatctgcgc ccggcgtgga tgattcgcat tggtctgttt atgtacgatc
     4141 atctgggtaa acgcaccagc ctgccgggat caactggttt gcgttttggc gcaaattcag
     4201 tgttaaaacc ggaaattaag cgcggattcg aatattctga ctgttgggta gacgacgccc
     4261 gtctggtact cgccaacgcc cagatggtgg tgcgtaaagg cggcgaagtg cttactcgga
     4321 ctcgcgccac ctctgctcgc cgcgaaaacg gcctgtggat tgtggaagcg gaagatatcg
     4381 ataccggcaa aaaatatagc tggcaagcgc gcggcctggt taacgccacc ggcccgtggg
     4441 tgaaacagtt cttcgacgag gggatgcatc tgccttcgcc ttatggcatt cgcctgatca
     4501 aaggcagcca tattgtggtg ccgcgtgtgc atacccagaa gcaggcctac attctgcaaa
     4561 acgaagataa acgtattgtg ttcgtgatcc cgtggatgga cgagttttcc atcatcggca
     4621 ccaccgatgt ggaatacaaa ggtgatccga aagcagtgaa gattgaagag agtgaaatca
     4681 attacctgct gaaagtgtat aacacgcact ttaaaaagca gttaagccgt gacgatatcg
     4741 tctggaccta ctccggtgtg cgtccgctgt gtgatgatga gtccgactcg ccgcaggcta
     4801 ttacccgtga ttacaccctt gatatccatg atgaaaacgg caaagcaccg ctgctgtcgg
     4861 tattcggcgg taagttgacc acctaccgca aactggcgga acatgcgctg gaaaaactga
     4921 cgccgtatta tcagggcatt ggcccggcgt ggacgaaaga gagcgtgctg ccgggtggtg
     4981 ccattgaagg cgaccgcgac gattatgccg ctcgcctgcg ccgccgctat ccgttcctga
     5041 ctgaatcgct ggcgcgtcat tacgctcgca cttacggcag caacagcgag ctgctgctcg
     5101 gcaatgcggg aacggtaagc gatctcgggg aagatttcgg tcatgagttc tacgaagcgg
     5161 agctgaaata cctggtggat cacgaatggg tccgccgcgc cgacgacgcc ctgtggcgtc
     5221 gcacaaaaca aggcatgtgg ctaaatgcgg atcaacaatc tcgtgtgagt cagtggctgg
     5281 tggagtatac gcagcagaag ttgtcgctgg cgtcgtaagg atgatgcccg gttgtgaagt
     5341 gcaaccgggc tatttggatt ccttagacta ttcattgtaa ataattgaat aacggcccct
     5401 gagctaaaat cgccaaactt aagcacgtta cccaatcgcc ccacttcttc ctctggtttc
     5461 tattttttct gcaccaatgg caataatatc ccaatgcaaa accaagctgt gcactaatcg
     5521 ggccgacaaa atcagtccaa ggctgcggcc aaaaagggga aaaattcagg ggaagccaga
     5581 caaacgtcag aaagaaattt cggatatttt ccagcaacgc tgatttctct ggagaaatat
     5641 aaagaatgaa attcgagaga attaaccaga aatagaccgg cgaaaagacc agccagcaat
     5701 gcagtctgtt ttttaattca ggaggcgttc tgcgggagaa ttgccaaccg atgccagcca
     5761 ttacactgaa aaaaagtgca tattcaacca ttaactcaag ttgtagttcc tgaagagaaa
     5821 tacgcacaac caggagcgga aaggagaata ttatttctgc cagaaaagtt aacgtgaata
     5881 taagtacggc taagacaata accgtcggcc ataccaataa tggcacgata acaaaaccga
     5941 gaattacagg gggaatataa agccggaatt taatcatttt tattttcctg gtgattcgga
     6001 tgatgcgtca tacatcgcgc ataaacactc atcaattatc cagtcgtcta cccagacctg
     6061 atgctgtgtg ccctgttctg gctgctttgg tgggttctca atcgcctgcc gcgcgcgttc
     6121 tttcaagatg gattgattgt tgaaggccca ggcattgatt tgggtggtaa tgacgttatc
     6181 gatatcgtcc tggttatctc tgatataatc ctcacaatat ctgttcaata aacaggcaag
     6241 agaaacggtc gaccaaaagc cgggaactga attaatggca tccagcaatg gcgcaagagc
     6301 gatatcggca cggaaaaaca tttccagcat atactgtaat tcgacagctt catcgccgga
     6361 tacccaatca gtcatatact ggcaggcaaa tcgttgcaaa attttccatt catcctcacg
     6421 ccaattttct ttactggcaa ggtttatacg ccgtagagaa aatacttcgt gaatattgtc
     6481 aaactgcccc tgtgcaacaa attccagtaa acgcggcaga aaatagcgga cctcatcgct
     6541 attttgcgga tcaggccctg ggctactatt ccatgcattg attaaggaat acgagatcgc
     6601 tcgtaacgat gtactccgta ataccttttg ttcctgctga ctcaggcaat attcacaaca
     6661 aaccacaaat ttctgcggca ccggataacg acgaaaaata tgataaatct gctcaattaa
     6721 tgcctgcatg gcggggctgg gttgcataat taccccctcg cccgctgatc gcgtgtaaac
     6781 ggttgataaa caaaacgagc attcgccacg ttcaggcgaa taatggcact acgagagagg
     6841 gaaaagctgg ggcgaatgca actattgcag tctcgacagc tttcttcagc gcaattttcc
     6901 actgtgtaat aacgagcgta gagtttcagt tccgcttgct tatcacgaaa tccgcgtaaa
     6961 ctgttatcgt tatgaaattt ccaggcatct ttaaagacct gaaaaatgta gatatcctca
     7021 atccaggact cacatttttt aagcgggcgc tttgctatga tcactcgctc atccgggttt
     7081 agcatcgccg taaattgaac ggtgaggtca tttgtcgtgt tcagatcaca ttgaacatct
     7141 ggtacatcac tgatataggg gtcaaatcca aagtcagcac cacaggcaca aagacggcta
     7201 atggtggcgg tatcgaggga aaatcccggc catacattgc tggacgttaa gccaatattt
     7261 aatgtgacat tatatcgaag caatccctca agaccgtcag caccgacgaa cttttcccct
     7321 tcatccaagg cctggttaat gagttgctga acaatgcctt catcccagta tgccgatttg
     7381 cagttggcaa tcgtaatatc cacgcctgtg aatggatcag taccgacaag gcttgcaaat
     7441 aatgtattaa ctaattccat ttttattatc cgccttaatg caacgtttta tacatcggca
     7501 cataacctac atcttccacc aggctctgcc cctgcggcgt taaaaaccac tccaccaatt
     7561 tttgtgtttc tgacgtcata ttttcccgcg ttaccataaa ggcatcgacg atatagggat
     7621 atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct agcagtttta
     7681 tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt
     7741 ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt gccacttctg
     7801 tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc accgtctggc
     7861 taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg ccaacagtac
     7921 gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc agggaattaa
     7981 ccgggttatc tacattgacg atgaaaacaa acgcttcacg ggcaaaaggc gtgtaaatca
     8041 aagtgacgcc cgattcctcc gcgcgttttt tctgcccacc ggaaggttga gccacaaaga
     8101 taatatcggc attaccttta acaatcttgt tatatgcttc gggagtacga gagttcgcca
     8161 gatattccca ttcgtgaaaa tcttccggca aaacgcttag tgcataaaag gcagaggcat
     8221 aaatggggta cgccgccgtt gcgccatcaa gtcgcggcca gttttgcgtg aactgaattt
     8281 gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc caggcccagg
     8341 tatcaatatt ttcacgcaca ccgacgccgg gattaagctg tttatataaa taagcctggt
     8401 aaccggcgac aaaaccaagc atgagaagga taaacaagag agaattacgg caacgtaacc
     8461 cggacgtgcc cgttaaagga tatctggtcg gccagccgta tcccagcgaa aaactgatat
     8521 gcgcgccaac aggtataatg actaaccacc agtactcacc accgactaat gaggctaaca
     8581 aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgctcccatc tggcgaaatt
     8641 gataatctgc cagatgcagc gcgattatcc agacccccag ggttaataat gcgggtacta
     8701 aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta tcgtccgccc
     8761 acactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa atgttgagca
     8821 gaaggtaaat tgttggcgtg ataaagatat tacaaacttc aataataccg gcaagtccga
     8881 cacccagcat ggcaataacc gccgccaaaa actgcgccag aatggggatg ccgaaaaaag
     8941 tcattaccag cgaggtcaaa atccattttc tgttttgcat tattctttcc attctttctg
     9001 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
     9061 ggcgaaaaaa acgggaccct ttggccccgt tctatttatt agtgaaatta caatctcacc
     9121 ggatcaatat gccagatatg atcggcgtac tctttgatag tacggtcaga agagaagtag
     9181 cccatattgg caatgttcag catcgctttt gcggtccact cttcctgaag ctcgtagagt
     9241 tcgtcgactt tatcctgaca atcgacatag ctgcgataat ccgccagtac ctggtagtga
     9301 tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc gacccggatc ttccggactg
     9361 aatacaccgc tgccgatttg cgtcagcacc tgatgcagct cctcatcttt ctcgtagtat
     9421 tcacgcggtt tgtagccctg acgacgcagt tcttccactt cttccgctgt gttaccaaag
     9481 ataaagatat tgtcagcacc gacatgatcc agcatctcga cattcgcacc gtccaacgta
     9541 ccgatagtca gcgcaccgtt aagcgcaaac ttcatgttac tggtgccgga agcttccgtc
     9601 cccgccagcg aaatctgttc agacagatcg gccgccggaa tgatcaactg cgccaggcta
     9661 acgctgtagt tcgggatgaa cacgactttc agcttgtcgc caatctgcgg atcgttgttg
     9721 atcactttcg ctacgtcatt gatcaaatga ataatgtgct tcgccatgta ataggccgaa
     9781 gccgccttac cgccaaaaat attcacgcgc ggtacccact tcgcatccgg gtcggccttg
     9841 atgcggttat agcgggtaat cacatgcaac acattcatca attgacgttt gtattcgtga
     9901 atacgtttga tttgtacatc gaacaacgcc tttggattca ccaccacatt cagctgctgg
     9961 gcgatatact ctgccagacg ctttttgttc tccagcttcg cctgatgcac agcgtgatta
    10021 accattggga aatcacagtg ttgttgcagc tcattaagca ggctaaggtc ggtgcgccag
    10081 ttacggccca ggtgttcgtc cagcacggct gaaagcgatg ggttcgctac cgctagccag
    10141 cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac ccgggaagat tttcgcaaag
    10201 tcggcaaaca acgattgcac catcagatta gagtgcagtt ccgatacacc gttaactttg
    10261 tggctcacaa caaccgccag ccaggccata cgcacacgac gaccgttgga ttcatcaatg
    10321 atcgacgccc gtcccagcag atcggtatcg ttcggatact gttcctgcaa ggttttcagg
    10381 aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg gcagaatttt acccagcata
    10441 tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt ggttagtgta ggagaagacc
    10501 tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt gctcatcgat cagcagacgc
    10561 atcatctcag gaatcgacag taccggatgg gtatcattga gatgaatcgc gattttatcc
    10621 gccaggttat cgtaggtttt atgcaactga taatggcggc ttaaaatgtc ctgaatggtc
    10681 gaggaaacca ggaagtattc ctgacgcagg cgcagctcac gcccggagta ggtggagtca
    10741 tccggataca gtacgcgaga tacgttctcg gagtggtttt tatcttccac tgccgcgaag
    10801 tagtcaccct ggttgaattt accgaggtta atttcgctac tggcttgggc actccacaaa
    10861 cgcaacgtgt tggtcgcgtc ggtgtcgtaa ccagggatta tctgatcgta agcgactccc
    10921 agaatctctt cggtttcaat ccagcgcgtt tttttacctt cctgctgaat gcgaccacca
    10981 aaacggactt tatagcgcgt gttgtggcgt ttgaattccc acgggttacc gtattccagc
    11041 cagtagtctg gcgactcttt ctggctaccg ttaacgatgt tctgcttgaa cataccgtag
    11101 tcatagcgga tgccgtaacc gcgccccggc aaccctaacg tcgccagaga atcaaggaag
    11161 caagccgcca gacgtcccag gccaccgtta ccgaggcctg ggtcattttc ttcatcaatc
    11221 agctcttcga gatttaaccc catcgcttcc agtgcgccct gtacatcttc gtaaattcct
    11281 agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa actccatcga caggtagtaa
    11341 acctgacgag tttcttgcga caactgggca cggtttgaac gtaaccagcg ctccacgaga
    11401 cgatcgcgca cagcaaataa cgttgcgttc agccattcat gtttattggc gacgaccggg
    11461 tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt gcttaagagc ttctacgcta
    11521 agcgtgggcg atgaatatgt aaacggagca ttcatatagg cgtttcctga aaactatttc
    11581 aagcgatagt aaagctcacg gtacgacttc gccgcgacct gccagctaaa atccattgcc
    11641 atagcctgac gttgcacaaa ccgccacagt gaaggacggg accacagtac aaaagcacgt
    11701 cgaatagccc gtaacagcga ccaggcatta ctatcttcaa agacaaaccc actggcgacg
    11761 ccatctgcaa ggttctcaag agaacagtca gaaaccgtat cagcaagccc accggtgcgc
    11821 cgcactaacg gcagcgtacc gtacttcaat ccataaagtt gcgttaagcc gcacggttca
    11881 aaacggctgg gcaccagaat gacgtccgcg ccgcccataa tgcgatgcga aaatgcttcg
    11941 tgatagccaa tctgaacgcc cacctggccg gggtattccg ctgccgccgc aaggaaacct
    12001 tcctgcagca ccggatcgcc cgcgccgagt agcgccagct gcccgccctg ctccagaaga
    12061 cccggtaagg cttccagcac caggtcgaga cctttctggc tggtcagacg gctcaccact
    12121 gcaaaaagcg gcactttatc gtcaacctta agccccattg cgatttgtaa ctggcgctta
    12181 ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg aggccaacag taagtccgtc
    12241 tctggactcc agattttctc gtccacgccg ttcagtacgc cggaaagacg cccttcacgg
    12301 tgacgctgtt gcaacagacc ttccataccg taggcaaact gcggttcggt gatctcgcga
    12361 gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt acagaccggc cttcaggaaa
    12421 gagatttgtc cgttgaattc cagcccatga atattaaaga atgaccatgg caattggatg
    12481 tcattcatgt gatgtgcata aaacatgcct tgataggcca agttgtgcac agtaaacacc
    12541 gacttcgccg gacgcccgcg cgccgccaga tacgcaggcg caaggcctgc atgccagtcg
    12601 tgcgcatgca ccacatcagg acgccagaat gggtcaagcc cgctggccat ttctgcccca
    12661 acccacccca gcagcgcaaa acgcaatacg ttgtcggtat aggcaaataa gttggtatcg
    12721 tgatacgggc ttcccggacg atcatagaga tgcggcgcgt caatcaggta tatgccaacc
    12781 ccgttgtaat gaccgaacaa cagcgtgata tgtccggcga aggtatcacg acgggatact
    12841 acctgcgcat cggtcacgcc acggcgaata tcgggaaatg caggcaacag tacgcgagcg
    12901 tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa taacatcagc cagaccgccg
    12961 gttttaagca gcgggaacat ctctgaacat acatgtaaaa cctgcattat cgctcctgtt
    13021 tatgccctaa cttccgtagc atttcgcgcg ttaccagcac gatgccttct tctgaacgat
    13081 agaaacgacg tgcatcttcc tctgcgtttt caccaatcac catgccttcc ggaataacac
    13141 aagcacgatc gatgacgcag cggcgcagac ggcacgagcg acctacccat acttccggta
    13201 acaatacggc ggaatcaatg ttgcagaatg aattcacgcg aacgcgcgag aacagaacgg
    13261 actgcaccac caccgaaccg gagatcacac aaccgccgga taccagtgag ttaagggtca
    13321 tcccgtggct accggagcga tcctgcacga atttcgctgg cggtaatgat tcattgtagg
    13381 tgcgaattgg ccaattgcga tcgtacatat ccagttccgg caccacagag gccagatcga
    13441 gattcgcttt ccagtaagct tccagcgtac ccacatcgcg ccagtacggc tcggcatccg
    13501 ggtcggattg tacgcaagag agcgggaacg ggtgcgcata ggccagaccg gcttcggtga
    13561 tcttgggaat caaatctttg ccaaagtcgt ggctggagtt ctcatcacga tcgtcttctt
    13621 ccagcagttc atacagatag tcggcgtcaa agacgtagat acccatactc gccagagatt
    13681 tgctcggatc gttcggcatt gacggtgggt tagcaggttt ttcaacgaat tcgataattt
    13741 tatcgttctc atcaaccgcc ataacgccaa atgcggaggc ttcttcaatc ggtactggca
    13801 tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc gataagcata cgcgagtagt
    13861 cttgcttgta gatatggtcg cccgccagga tcaccacgta ttccgcttta taacggcgga
    13921 taatgtcgag gttttgggtg accgcatctg cggtgccgcg ataccagttt tcccctttca
    13981 ttctctgctg tgctggcagc agatcgacaa actcgttcat ttcttcattg aagaatgacc
    14041 agccgcgctg aatgtgctgc accagagtgt gggactggta ctgggtgatc acgcccatac
    14101 gacggatccc ggagttgatg cagttagaca gcgcaaagtc gataatgcgg aacttaccgc
    14161 cgaagtgtac ggccggtttt gctcgcttat tggttaaatc cttcaggcgg gtaccacgtc
    14221 ctcccgccag tatcagggca acagatttca atggcagctg gcgcgccaac attaagtgat
    14281 cgttcttctc taaactaacc atgactaact ccttttttat catctctgga acacacacaa
    14341 tccgtgtgca ggtccctgcc agacagccgt aatcactggg ttatcctctc cagcgaatgg
    14401 gggaatggcg tgccactccc cagcaggtaa aacaatctct gttacctcaa gcgtggcgtt
    14461 aattgcgatc aaaaagcgat ccgagagcag aatttgcagc tgtttcggcc cgttttgcca
    14521 ctcatccgtg cttaaaggtt gagcatatcg atttagccaa cggacattgc cgtcgccttc
    14581 ttcccaccag cgattctcca ccaaagcggg aatgcgcttg cgcagatgga ttaacgcggc
    14641 ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc aaccaggtta attggttatc
    14701 ctgacagtag gcattgttat tgccatgctg gctgtgacca tgttcgtcac cggccagtaa
    14761 catcggcgta ccctgggaga gcaacaacgt tgttaacagg gcgtgaatgc tgtcgcgccg
    14821 ccgttcaacc aggtcaagag aaccgcctaa cccttcttta ccatgattgt tactgtaatt
    14881 gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca ttgtgtttat ggttgaagca
    14941 aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg agattaatcg cggcactcgg
    15001 cagacgacca ttacgtttaa aaacatcgct ggaggcagca aaacgcccgg caaacgcccc
    15061 cagaggcaaa tcataatgta gccagaaacg acgggcagca tcgcggaaat gatcgttcca
    15121 ctcggcaaac agcggcggga aatttcccac ctgataacca ccaggagcga tatcccacgg
    15181 ttcagcaatt aacttcacct gcgagagcac cgggcagttc tggatagcgg taaacaacgg
    15241 cgcatcctga cggaactctg gcgtacggcc catgactgcc gccagatcaa agcggaaacc
    15301 atcgacgtgg caggtttcta cccaataacg caggcaggcg ctggcataat ccaccaccgc
    15361 cggatgactc aaattgagcg tgttgccgca accggtccag ttgtgataat cgccgtcttc
    15421 tcttatccaa taatagctac ggttatcgat cccacgcagc gagaataacg ggccgtcgag
    15481 gtccagttcc gcactatggt tgagcacgat atcaagaatg acttcgatac ccgctttatg
    15541 cagtgctttg attgcatcgc gaaactcatc cagcgccgtt tctggcgagc aggcatacgc
    15601 cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag tagttactta gccccatgcg
    15661 ttgcagacgt ggttcactgg caaactgcgc caccggcagc agttccagcg cggtaatgcc
    15721 caactgtttc aaatagttga tcatcaccgg atgcccgagg gctttataag tgccacggat
    15781 ctcgaccggg atctccgggt gcaagtacgt taatcctttg acatgggctt cataaatgat
    15841 ggtgctgccc cacggcgtgc gcggcggggc atcatcttcc cagtcatagt gatcaaccac
    15901 cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag tcaggttcat tatgaccggc
    15961 gtgcagcagc gggttatctt taaactcccc gtcaatttgc cgcgcgcaag gatcaatcaa
    16021 caacttcgcc gggttaaagc gatgcccctc ggcgggttgc caggggccat gaacgcgata
    16081 accataacgc aaacccgggc gcgcatccgg cagataaccg tgccaaatgt cgccactgtg
    16141 ccctggcaag tcatagcgat gttcctggcc attggcgtca aagacacaca gttctacccg
    16201 ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga ccgtcgtaat gcgcgccgag
    16261 gggagcgggt ttgccaatgg cgagtcgtgt cattctgcct cccgaaccag ccagatagtg
    16321 gccagcggtg gtagtgtcag gcttagtgaa tgctgacgac cgtggctggc aatctcatcg
    16381 ctgtgtaccg tgccgccatt gcctgcatta ctgccgtgat agtgcatgga atcggtattg
    16441 aggatttcac gccatttgcc tggctgatta atgccgaagc gataatcatg acgcggcacc
    16501 ggcgtaaagt tactggcaac gataatttcg ttaccctctt tatcgcgacg cacaaagatc
    16561 agcaccgagc gttctttgtc atccaccacc agccattcaa agccgtacgg gtcaaaatcc
    16621 agttcatgca ttgctttatg gtggcggtag gtgaggttca gatcgcgcac cagacgctgg
    16681 acaccgtggt gccagttatc gccgccttcc aacagatgcc agtcgaggct ggcgtcatgg
    16741 ttccactcac ggccctgggc aaattcgttc cccatgaaca gcagtttctt gcccgggaat
    16801 gcccacatcc agccatagta ggcgcgcagg ttcgcgaatt tctgccatgc gtcgcccggc
    16861 atgcggtcga gaatcgattt tttaccgtgg accacttcat catgcgacaa cggcaggacg
    16921 aagttttcag tgtagttgta gagaatcccg aaggtgagtt tatcgtgatg atactgacga
    16981 taaaccgggt cgagcttcat gtagtccagg gtgtcatgca tccagccgag gttccacttg
    17041 taccagaagc ccagaccgcc catatcctgc ggacgagaaa cgccagggaa atcggtagac
    17101 tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa gaatacggtt ggtattacgc
    17161 aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt cgttcgggat ccactccccc
    17221 tctttacggc tgtagtcgcg ataaatcatt gacgccaccg catcgacgcg cagcgcatca
    17281 ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga ggaagttact gacttcacgg
    17341 cgaccatagt tgtagatcag cgtgttccag tcctgatgat agccttcacg cggatcgctg
    17401 tgttcataca agttcgtgcc atcaaattcg gcaagcgcaa agtcatcagt cgggaagtgg
    17461 cctggcaccc agtcgagaat cacgttcaga ccagctgcgt gtgcggcatc aatgaaataa
    17521 cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat acaggccggt tggctgataa
    17581 ccccaactgc catcgaaggg atgctcgtta atgggcagta gttcgaggtg ggtaaagccc
    17641 atccatttag cataaggcac cagttgatcg gccagctcgc ggtagctcaa ccagaaattg
    17701 ttgtcggtgt ggcgacgcca ggaacccagg tgaacttcat aaatagagat tggcgcatca
    17761 aactgattcg cttttttgcg ctcttcagtc tgtacaacct tttccggcag cccgcaaata
    17821 agagacgcgg tttccgggcg catttgcgct tcgaaggcat aagggtcgga cttcagacgc
    17881 aagttgccat tggcatcaat catctcgtat ttatagaact gaccgttatg cgccccaggg
    17941 ataaacagtt cccagatgcc gctctcttta cgcaggcgca tcgggtgacg gcgaccgtcc
    18001 cagtagttga attgcccaac caccgagacc cgacgggcgt ttggagccca gacagagaaa
    18061 cgcgtacctg tgacgccatc catagtatct gcatgcgcgc ctaaggtttc atacgggcgc
    18121 aggtgagtac cttcagataa tagccaggca tccatttcct ggattagcgg accaaaacgg
    18181 taaggatcat caatcaggtt ttgctgacca tgccagacaa cagccaactg atagcggaaa
    18241 aaattcttac gtcgcggaat gacgccgcta aagaatcccc gtgagtcgag acactccagt
    18301 tttgcgagtt tgcgcccggt tttcggttca atcacccaca catcggtagc gtcgggtaaa
    18361 agggcacgga cttccagtcc cgcggtggtt ttatgcattc ccagtacgga aaaaggatcc
    18421 gcaaaatggc ctgcaattag cgcgttaatc acgtctctat cgatacgatc ggacatgctt
    18481 gtcttcctgt tttattgtgt caccccatcc aattggattt ttgacttctg gttgtgacat
    18541 ttttttgacc tgaacggcgc agcactctgt gcatcctctc tgcgtcgtcc tcacttcagg
    18601 taaggctgtg aatactcatg tattcagcca cccttaaaga atagccaatg ctctatttaa
    18661 ctcccggtaa atcatgaaac atctgcgctt actcctgtat tacgcactaa caggggcggc
    18721 atcgcgcccc agatttaatg aataaagatt acgccagttg acgaagcatc cgacgcagcg
    18781 gctccgcggc cccccacagc agctggtcgc ccacggtaaa ggctgacagg aactctggtc
    18841 ccatattcag cttacgcagg cggcctaccg gcgtggtcag cgtgccggta acggcagctg
    18901 gggttagctc acgcatagtg atttcccgat cgttcggaac gactttcgcc cacggattgt
    18961 gcgcagccag cagttcttcc acggtcggaa tagacacatc ttttttcaat ttaatagtga
    19021 atgcctggct gtggcagcgc aatgccccga cacgcacaca taaaccatct accggaatta
    19081 cggaagatgt gttgaggatc ttgttggttt ccgcctgccc tttccactct tcgcggctct
    19141 gaccgttatc gagctgtttg tcgatccacg gaatcaggct acccgccagc ggcacgccaa
    19201 agttatccac cggcagctca ccgctacggg ttaaggttgt gactttgcgt tcgatatcga
    19261 gaatagcaga ggacggggtc gcgagttcat ctgccacatg gccatacaga tggcccatct
    19321 gggttaataa ctcacgcata tgtcgcgcac caccgccgga agcggcctgg taggttgcaa
    19381 cggacaccca atcaacaaga tcattggcga ataaaccacc caacgacatc aacatcaggc
    19441 ttacggtaca gttaccgcca acaaaagtcc tgatgccatt atttaatccg tcggtaatga
    19501 cgtcctgatt gacggggtca agaatgatga tggcgtcatc tttcatgcgc agagacgatg
    19561 ctgcgtcaat ccagtaacct tgccatccgc tttcacgaag ctttggatag atttcgttgg
    19621 tataatcgcc gccccgacag gtcacaatga tatcgagggc ctttagcgcc tccagatcaa
    19681 aggcatcctg aagtgtgcca gtggttccgc caaaagacgg cgcagcctgg ccaagctgag
    19741 aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg ctcttcaacc atgcgttgca
    19801 tgagaacgga gccgaccata ccgcgccagc cgataaaacc aacatttttc ataagcgttt
    19861 ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac
    19921 catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta
    19981 atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg
    20041 agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg
    20101 aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat
    20161 catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga
    20221 gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat
    20281 tctgtttcta attgccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct
    20341 tccggcaggt gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg ccgggccgac
    20401 tattctcgcc acgctgatgt tgttgtctca tcagtacccg aatcagatgg ggcatctggt
    20461 gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt
    20521 tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct
    20581 ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaagg
    20641 ttcaaacagg tgctgcacac aaccagacgc ggcacctgtt ttattacctg aactgataat
    20701 gaccttcgcc caatcggata acccttccct cttcttctgc gcggttcagc cacacactaa
    20761 tttgttcgat ggttaaacca gtacaagacg acagcctttc aatacttatc gattcctttg
    20821 caaagatcgc cagttccgcc acaaagagtt gataaaaatc gacaggcgta tgcgcgggca
    20881 ttactgcgta atttgcgtca tcttcatgca gcgaaaacag ttctagttga gcgttacgga
    20941 gtgctctgtc atacatcagc ccaggaacgt ctgacagtct gatcggcaca ttctcagcct
    21001 gttcagcact ccatgacgcc ccattttcta ctaatcgtga attggctgat tgcatatcct
    21061 gatttggttt aacctgcaca ggcagccact gatgttttaa tgcctctatc gccccggtta
    21121 tcgttccacc ggtcattccc gcacgtacaa ccattgcgct ttcagcaagg caataaatat
    21181 atttatttcg cgccatcgca ttgccgacgg tgaaacgcac ctctgggtaa aacggcgaaa
    21241 tcaacaccag gttgcctgct ataagccctt cacgccattt cactaacgta ctcgttttta
    21301 acaagctatc agctaatacg ccaacggcag ttcccccggc ctccagtgcc gacgccattg
    21361 cacattcatc aatacctcgc gcaccaccag agataacgca aatcccctgt tgggccagtt
    21421 tcgcggccag ttgttgggta tagcgcaaat cgtcagtcgg agcatctcgc gagccaacaa
    21481 tcgccatacc ttccgcttgc agtaatgctt tattcccgca gccaaataaa acgggaggtg
    21541 catccgttcg caatcggttt ttcaagcgaa cgggataatc agcatctcct cgggttatga
    21601 tccataaacc ggcgcgttgc cacttatcta ccgccaacgc cagactatga ccgcgcgcca
    21661 gcaagccaag aatacgctcc tgagaaatac gcggatcgct ccagcctgta agtttctctt
    21721 gcggttgcgg caccagaagc tcggcgggac tgatacgttg gtgcttcagc cataatgcaa
    21781 acttccccca ttcactatta ctaagaggtt tatgctcact cgccgccgcg cgagaaaaat
    21841 cgctggttag caggagagtc gcttgtgcat tggctgaaag attcatcaat tttttaccga
    21901 ggtagaagca agggcaagag gataaaccgt cgggcaacct gcctggcgta acagtgctgt
    21961 cagaactgtc agcgtccatg cagaatccac gatatcgtca accagcaacg ccggacctgg
    22021 catcaaagga ggggtaatca caaacgcccc gtcgagattt tgacactggt gaaaacggtt
    22081 ttgctgcatt ttctgcggtg gattgtccac gactttttca atggcatcaa taaaaggtaa
    22141 gccaagtttc gccgccagtc gccgggcaaa atcaggaacc aggtcgaggt gccttaatga
    22201 aggaacgcag catacccagg ttggttcagg atgcggattc cagcgttggc gaaccatttc
    22261 cgcacatgcc tccaccagct catcactgaa tcgccccgtt tttttctcct gtgctacctg
    22321 ctttccccat ccggaatctc cccaacgaga aaggactcgc cccgtagatc cttgtaaacc
    22381 cgcaggcaag ttccctttaa atccgtattg cgtaaaagct ccagatgcta cctgtttatt
    22441 gagatttaat ggcaggtcag cgtgtttaat aaataaattt gccgcatgga gtaagccgct
    22501 atcaatatcg ggcgacaaca acggatgctg aagacaactg ctacatctac cacaacgttc
    22561 ggcacttggc tcatcgagtg cgcggcgcag aaattgcatt ttgcactcct tggtcgtgat
    22621 atagctttca acatctgcca gctcattttt tctctgattc atcaaatggt taattcgttc
    22681 atggtcagga gagaaactga cgatagttct gcgccataat ttctcggtat acaccacagg
    22741 cgatggattt tcagctacca ataatttcag tgctttttct atttgcccat aacggagatt
    22801 cgtccgttgt tcaatgcctc gtagggtaag accgtcattc tcgctaagta cgttgagtat
    22861 ttcatgaatt tgcgcctccg caggaaaggc gctttcacgg aagaatttat gaatagcgcg
    22921 gtcttcacca ccacaaagca atatgccaac agcggaatct atagcacgcc cggcacgccc
    22981 tacctgttgg tagtaaccga caatagaccc cggcatctgg taatgaataa caaaccccaa
    23041 atccggtttg tcaaagccca ttcccagcgc cgtcgtcgcg accagaactt taattttatt
    23101 tgccagcaac gcctgctcca gatattccct ggcagtatta gaatcttccg cgccttcaca
    23161 agtcacgccg ctgtagtaag caaatgcact gataccattt gtccttaacc actcggcgac
    23221 aaggtcggca tcgcgagtag tcagggtata aacaattccc gatttggaaa actgagggat
    23281 aaccgttgct aaccatgcca gacgggatga ctgttcacct aagactaagg catcaagcgc
    23341 caaactttcg cgagccagcg ttccacgctg aatcacaatg tcacccaatt gctgacggat
    23401 atcctcaacg acacggttat tcgctgtcgc ggttgtaccc agaactgggg tattcgcagg
    23461 cagttggcgc aaaatatcta atatacgtcg gtaatccggg cgaaaatcgt ggccccaatc
    23521 agagatgcag tgcgcctcgt cgaccacaag caagccaata cgatcggcta taggatataa
    23581 aaccgtttcg ataaagtctt ggtttgccaa acgttccggg gaaaccagca agcaatcgac
    23641 ctcaccttgc agcaacttat cgctaatacg ctgccattct tccctgtttg tagaattcaa
    23701 cgtttcagcc gtaataccta accgttcagc ggcggcaacc tggttacgca tcagggcaag
    23761 caaaggagag atgataattg tcgggccagc accgcgatcg cggaagattt tactggcgat
    23821 gaagtacacg gcacttttcc cccagccagt gcgttggaca accagcagtt ttcgtcgttg
    23881 gttcaccacc gcgtctatgg cttcccattg cccttccctg aaagtagcgg tggtatttcc
    23941 taacatccgc tgtaaaagca attcagctgc gtgcttttcc atctttatct cctgcgatct
    24001 ttaggcagtg attagaacgg attattagca ggtaaatttg cgagagggat caaaaagatg
    24061 aagagaggag ataagagaaa tgaattactg cgagcaagct cgcagtaatt tgtcgcagta
    24121 attaactcaa cagctggaac gcaatcatcc caacgatggc accgacagtg ccgaggatgg
    24181 tttccatcat ggtccaggtt ttcagcgttt cggcttcggt cgcgccggta aatttaccga
    24241 acaaccagaa accggcgtcg ttaacgtggc tgacaacaat cgaaccacca gcgatacaaa
    24301 tcgacagcgc cgccatttgc gcaccggagt agttcagttg ttcaataacc ggcatcacca
    24361 gtcctaccgc cgttaaacag gctacggtgg cagaaccctg aatgatgcgc actgcagcgg
    24421 ccagcacgaa gcaggtgatg gctatcggca ggcccatgcc agttaacgct tcgcccagtg
    24481 ccggacctac gccagagtca accagcacct gtttgaacac gccacctgca ccaatcacca
    24541 gcagaatgat ccccgccggt tgcagcgcgt gaccgcaaat ctccatcact ttgtcttttg
    24601 gcatgccctg acgcattgcc aggccataaa tcgctaccag acaagcaacc agaatcgcgg
    24661 taaacggatg accaataaac tcgaaccatt cgtaagcggt agagccttct ggcacaaaac
    24721 gcgcggcaat ggttttcagc cccaccagca ccagcggcaa caggatcagc gacaggctga
    24781 atccgaaaga tggcatcttg ccttcgccga gatgcggttc gctgatgtcg tcaggaatat
    24841 gcaactccac gtagcggctg atgaaattac cccacagcgg cccggcaata atcattcccg
    24901 gaattgccgc acacaggcca atcaggatca tccagccaaa atcggcgttc atctgcgatg
    24961 ccagcagcat tggcgctggc cctggcacca ggaacgccgc cgctgccgcc acgcctgcga
    25021 ataatgggat taccagcttc accaggttcg taccggtgtg gcgcgccatt gagaaagcaa
    25081 cgctaatcag cagaacaatc gccacttcaa agaacagcgg tagcgcacag accagccccg
    25141 caaggccgat ggcataatgc gcgcggctgt gaccgaagga tttaagcatt ttgacggcaa
    25201 tctgatcgac tgcgccggtt tcatgtaaga tcttgccaaa catagctccc agggcgacaa
    25261 ccaccgccag gaagccgagg gtgcctccca tccctttttc catcgtcgct gcgattttat
    25321 cgagcggcat accggaaaaa aggccagccc ccatggacac caccatcaaa gccaggaaag
    25381 cgtgcatacg cgccttcatg actaaaaaca gcagcagtaa aacagaccct actgctgtta
    25441 aaacaagcgt taatgtagtc actacttatt tgcctttttt aataacctca atggtgcttg
    25501 ccacaacacc ttccagcggt tgatcgatat ccaccaccag tacatcagtt tcgtccgcac
    25561 ccggctcctg cagcgtttca aactgcgtca ccaacatttg ggttttaaag aaatggcctt
    25621 tgcgcgcttt caggcggctt tcaatcacgt caaaatcgcc tttcaggtag atgaaagaga
    25681 gattcggatt accttcacgc agcaagtcgc gatagtgttt tttcaatgca gaacagacga
    25741 tcagtgacac tttattagtg cgctgcatag caaacgcggc gtcgttcagc gcctgcaacc
    25801 acggtttgcg atcgtcgtca ttcagcggtt cgccagacgc cattttttcg atattgcgcc
    25861 gtggatggag gaaatcgcca tcaagaaacg cggcatgaag ttgatgcgcc acttcactgg
    25921 cgaccgcaga tttgccgctg cccgatacgc ccatcaagac gtaaatgtgg tgatcatggt
    25981 tagtcgtgct caaagtggtg cccccacaat acaagaatta aaaatgttac gggtaactgt
    26041 tatcggtaac attgtccagc cggacaatat cagaagcaat atccatccgt gccttaagtg
    26101 tataagtgtg agctacttca aatttgtagg cttaaataga tccgcccggt gacaaggtga
    26161 aacctaaatc taacattttc ggtgtcacag attcgccacg aatacgcgcc agcaggcgtt
    26221 cagcgccaat actgcccatc cgctcacgcg gcgtcagcac gctcgctaac cgcggctcca
    26281 tcacctggcc aatgtcatga ccgtggaaac cggcaatcgc catatcgtca ggaaccttca
    26341 atcccagacg ctgacattca aacgccgcgc cgaccgccag gtcatcgttg gtacagaaca
    26401 cgccatccag ctgcggatat tcccgccgcg cctggcgaat cagttcaata ccggaagagt
    26461 atgaagaaga ttgctcaacc atcacgctat acggcaccag acctgcatcc agcatcgcct
    26521 gttcgtatcc cttctgtttg atgatagtac gttcgtcgag acgcgcgccg agataggcaa
    26581 tgtggcaatg cccgcgagca ataatggcgg tggtcatctg gcgtgccgct tcaaagttat
    26641 caaaaccgac ggcgatatcg aggcacggcg actggctgtc catgagttcc accaccggga
    26701 tccccgccac ttcaatcatc tttaaggtgc gcggcgtgtg ggtacgttcg gtgaggatca
    26761 gtccgtcgat attccatgag agcatggatt cgagacgttc ttgctccatt tccggtttat
    26821 aaccgtagtg cgccagcatg gtctgataac cgtgcgcgtc ggtgacgctt tcgattccgc
    26881 gtaatacttc cgcgaaaacc tggttggtga gagaaggtaa caggacgcca atcgcccggc
    26941 tggtggcgtt agagaggata tcgggcgcac gattgggaat atagcccagt tcatcaagag
    27001 cggcggcaat cttgccgcgt agagcgacgg aaacctgctc cgggttgcgt aaaaaacggc
    27061 tgaccgtcat tttggtcacg cctacacggt cagccacatc ctgaagtacg ggtcttttct
    27121 ttttcatcgt cctgaaggta caaaagagat agattctctt agtttaacac ggacgcatca
    27181 gaaccttccc ggggaaaaca gtgcttgttt acaattatta gatatacact aaatcaattg
    27241 catcgcattg tgctaacgac gcggcaaaac cccggaagcg tgcatcagca cgcgaccgga
    27301 gcgagcgagg aagcctgcaa aaaggcaggc ttcaaagatg ccgttgagtt ttaaaccggc
    27361 ggcagatcga acagtaacac ttcgctgtcg ctatccgcat ggatggagat tgcctgctca
    27421 tcccagattg ccagaccatc gctggtcgag gctttcacgc cgttaatggt gacattgcct
    27481 ttcaccacct ggatccagac gcggcgttca gcggcaatct gatgcaccga ctgctcatct
    27541 ttcagcaacg cccaacgata cagttccata tcctgatgca ctttcagcga accatctcgc
    27601 gcatccggcg agagcaccag ctgtttgccc tgcacggcat cgaagcgacg ctgttcataa
    27661 cgcggcgtaa tgccattttc ttcaggcatg atccagatct gatacagatg cagacgctcg
    27721 gtgctgtttg ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac
    27781 tcacccgccg gaacctgctc tttattgccc atgctgtcct gatgctcaac agtaccttcc
    27841 agcacgtagg tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctgct
    27901 tcaatcacgt cgtcgttaat cacgcgcagc gcggagaagc ccataaagtt cggatcgtaa
    27961 tagttggcaa aagagaaagt atgccaggag tccagccagc catgatttgc atgaccacgt
    28021 tcatttgctt tgcggaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt
    28081 agtgtggacc cgatctgcct gggatgatag agggtgaaaa ttgacccctc tgttcaaaaa
    28141 aattgaacta ttcgaggggc catcaaactt acttagcgag agttactgtg gagggagagg
    28201 cttgctcaaa tccgcgttca aggatttcca gattggtaag aacttcagat tccttgacgt
    28261 aatttggcgc accgtgggtg atggtttgat acaacgcatc ataaacgcgc ccgtaatcgc
    28321 ccatctccgg cttcatctct tctctgaccg tcacgccctc gtcattgaca tactccagca
    28381 caccgaccga atcatccgct gcgaatcccg gttcgcccgg cataatatta gccttcaggc
    28441 tggtttcctg ctggtcgata ccgtatttaa taaacgaacc tttcttaccg tgaacgataa
    28501 atttcggata atcgattttc accagatggc tggttttgac gatggctttc aggtcgccat
    28561 aaaacagttg cgcttcaaag gtgtcgtcag gattggcttt attacgcagg ctgcggatgt
    28621 cataagcgac gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac
    28681 caaggccata aaacgcgcca tcctgcggca gcccaggttt ggtctctgcc accgggcggt
    28741 aatagtcaaa atggctttcc acttcaacaa tctctcccaa cttgccactt tcaatcgctt
    28801 ttttcgctgt caggaagcag gagtcaaagc gacgattctg atacggcgtg acggtcagcc
    28861 ctttgctttt cgccaacgca aacagctcct tcgcctgggc aagcgtcggg gtgaacggtt
    28921 tttcgaccag cacatttttc ccggcttcca gcgcgcgttt cgcgtactcg aaatggctgt
    28981 ccgcgtgggt gcagacaaca accagcttaa catcgggatc gtttagtact tcgtcgagat
    29041 cgctggtgaa atggatatgg gaataaatgg gagcctgttc ttccggcttc gcatggcgac
    29101 gaaaaatatg cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac
    29161 gggtggtgct tttgccgaag ccaataaagg cgcagttgat gaccatgatt tcgtcctttt
    29221 taaggtggtt atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa
    29281 cgaacacaag cactcccgtg gataaattga gaacgaaaga tcaaaaaaaa gccagcaccc
    29341 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
    29401 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
    29461 tgcaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
    29521 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
    29581 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
    29641 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
    29701 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcgcccac
    29761 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
    29821 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
    29881 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
    29941 gctttgaaat tgaagggact ggtaagaagt acgcattgcg taatggtgaa tatgtcgatg
    30001 catattatat ggcgcgggtg aagtaagata gtgccctttt tctgagatgg aaaaagggtg
    30061 tcattcaaaa tcgacatacc ttcctttaag gtatttattt gcccaataca taaattgaga
    30121 tttaactcat aattacttcc taaagtgtaa tattttattt tttaatatat acgcctacaa
    30181 tttcctggag taaataaata acaattaaca agcataatat tgccattgat aaaatagcat
    30241 gccataaaag gacttttcag ggatgagtaa tattgtttac ctgacagtaa cgggagaaca
    30301 acaaggaagc atctccgcag gttgtgggac ttctgagtct acaggtaatc gttggcagag
    30361 cgggcatgag gatgaaatat ttacattctc actcttaaat aatattaata atacggggct
    30421 tggtt
//
LOCUS       DAZFFI010000052        29092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFFI010000052 DAZFFI010000000
VERSION     DAZFFI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.052"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..837
                     /locus_tag="ACHJ12_002685"
     CDS             <1..837
                     /locus_tag="ACHJ12_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4990450.1"
                     /translation="IVDGQQRVTTLVDFYKNKFALTSASKKYSGMYFNDLPDDAKRCF
                     ISYQVETSTVYSASRAELLEMFRRMNAYTSPLTSAEKRHATFQGAFKWYIVEKADTHA
                     ELLEIYNILSPKILARMGDAEFISDMAILIDRGLCSKSAPATEAIYKKYDDDFPLENI
                     YNERIDYFFNLLAEDFSELRNSFMMKSYVVHSLFCAITALKFGFPGSDELENELGFTA
                     NPNFDVNPSRVIPILLEMADAHEIQDEDGPYAAYVTSCLSSTTKLPQRTTRTKELIKA
                     FL"
     gene            846..1496
                     /locus_tag="ACHJ12_002686"
     CDS             846..1496
                     /locus_tag="ACHJ12_002686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990451.1"
                     /translation="MDKIYRNFIHRCSVYQGKVDILRFNRIEYDEWTHAKLTEGLMSD
                     LWQNWCLFCRNLIHKSCRGAVCADTTVFGPRVTQYGNSWQRLGYEAKNINGTIKNNGH
                     NSFLIRYEPTWGDIDKIVNIITHLNPKNKNCLLAAFGMGLKDINYLQLARNACAHKNV
                     ETIEELINKTKLFYDVTTLKKPSDFAWSLKKGTHSFAFYTWLYEMRIIAKNAILTC"
     gene            complement(1622..2872)
                     /gene="icd"
                     /locus_tag="ACHJ12_002687"
     CDS             complement(1622..2872)
                     /gene="icd"
                     /locus_tag="ACHJ12_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4990452.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            3044..3697
                     /gene="rluE"
                     /locus_tag="ACHJ12_002688"
     CDS             3044..3697
                     /gene="rluE"
                     /locus_tag="ACHJ12_002688"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4990453.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            3707..4168
                     /gene="nudJ"
                     /locus_tag="ACHJ12_002689"
     CDS             3707..4168
                     /gene="nudJ"
                     /locus_tag="ACHJ12_002689"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO4990454.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            4222..5328
                     /gene="mnmA"
                     /locus_tag="ACHJ12_002690"
                     /gene_synonym="trmU"
     CDS             4222..5328
                     /gene="mnmA"
                     /locus_tag="ACHJ12_002690"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4990455.1"
                     /translation="MSETAKKVIVGISGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            5364..6005
                     /gene="hflD"
                     /locus_tag="ACHJ12_002691"
     CDS             5364..6005
                     /gene="hflD"
                     /locus_tag="ACHJ12_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4990456.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            6009..7379
                     /gene="purB"
                     /locus_tag="ACHJ12_002692"
     CDS             6009..7379
                     /gene="purB"
                     /locus_tag="ACHJ12_002692"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4990457.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFQQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            7547..8218
                     /gene="phoP"
                     /locus_tag="ACHJ12_002693"
     CDS             7547..8218
                     /gene="phoP"
                     /locus_tag="ACHJ12_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4990458.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            8218..9678
                     /gene="phoQ"
                     /locus_tag="ACHJ12_002694"
     CDS             8218..9678
                     /gene="phoQ"
                     /locus_tag="ACHJ12_002694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4990459.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVVR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            9754..10875
                     /gene="roxA"
                     /locus_tag="ACHJ12_002695"
                     /gene_synonym="ycfD"
     CDS             9754..10875
                     /gene="roxA"
                     /locus_tag="ACHJ12_002695"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4990460.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(11021..12250)
                     /gene="pepT"
                     /locus_tag="ACHJ12_002696"
     CDS             complement(11021..12250)
                     /gene="pepT"
                     /locus_tag="ACHJ12_002696"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4990461.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            12500..13636
                     /gene="potA"
                     /locus_tag="ACHJ12_002697"
     CDS             12500..13636
                     /gene="potA"
                     /locus_tag="ACHJ12_002697"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4990462.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            13620..14477
                     /gene="potB"
                     /locus_tag="ACHJ12_002698"
     CDS             13620..14477
                     /gene="potB"
                     /locus_tag="ACHJ12_002698"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4990463.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            14474..15268
                     /gene="potC"
                     /locus_tag="ACHJ12_002699"
     CDS             14474..15268
                     /gene="potC"
                     /locus_tag="ACHJ12_002699"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4990464.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            15265..16311
                     /gene="potD"
                     /locus_tag="ACHJ12_002700"
     CDS             15265..16311
                     /gene="potD"
                     /locus_tag="ACHJ12_002700"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4990465.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            16369..16830
                     /locus_tag="ACHJ12_002701"
     CDS             16369..16830
                     /locus_tag="ACHJ12_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO4990466.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            16827..17615
                     /locus_tag="ACHJ12_002702"
     CDS             16827..17615
                     /locus_tag="ACHJ12_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4990467.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(17754..18575)
                     /gene="cobB"
                     /locus_tag="ACHJ12_002703"
     CDS             complement(17754..18575)
                     /gene="cobB"
                     /locus_tag="ACHJ12_002703"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4990468.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQNGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(18591..19502)
                     /gene="nagK"
                     /locus_tag="ACHJ12_002704"
     CDS             complement(18591..19502)
                     /gene="nagK"
                     /locus_tag="ACHJ12_002704"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4990469.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(19531..20775)
                     /gene="lolE"
                     /locus_tag="ACHJ12_002705"
     CDS             complement(19531..20775)
                     /gene="lolE"
                     /locus_tag="ACHJ12_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4990470.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(20775..21476)
                     /gene="lolD"
                     /locus_tag="ACHJ12_002706"
     CDS             complement(20775..21476)
                     /gene="lolD"
                     /locus_tag="ACHJ12_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4990471.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(21469..22668)
                     /gene="lolC"
                     /locus_tag="ACHJ12_002707"
     CDS             complement(21469..22668)
                     /gene="lolC"
                     /locus_tag="ACHJ12_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4990472.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            22930..24003
                     /locus_tag="ACHJ12_002708"
     CDS             22930..24003
                     /locus_tag="ACHJ12_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4990473.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24131..27577
                     /gene="mfd"
                     /locus_tag="ACHJ12_002709"
     CDS             24131..27577
                     /gene="mfd"
                     /locus_tag="ACHJ12_002709"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4990474.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKSGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            27721..28683
                     /gene="ldtC"
                     /locus_tag="ACHJ12_002710"
     CDS             27721..28683
                     /gene="ldtC"
                     /locus_tag="ACHJ12_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4990475.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(28766..29023)
                     /gene="bhsA"
                     /locus_tag="ACHJ12_002711"
                     /gene_synonym="ycfR"
     CDS             complement(28766..29023)
                     /gene="bhsA"
                     /locus_tag="ACHJ12_002711"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4990476.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         7235 a         7409 c         7263 g         7185 t
ORIGIN      
        1 attgttgatg gccagcaacg agtaacaact ctcgttgatt tttataaaaa caaatttgca
       61 cttacttcgg catcgaagaa gtattctggc atgtatttta atgatttgcc cgatgatgca
      121 aaaagatgtt ttattagtta tcaagttgaa acatccacag tttattcagc aagcagggct
      181 gaattactcg aaatgtttag acgaatgaat gcttatacat ctccactaac atcagcagaa
      241 aaacggcatg ctacttttca aggtgcattt aagtggtata tcgttgaaaa agcagatacc
      301 cacgctgagc ttttagagat ttataatata ttgtcaccca aaatattagc cagaatgggt
      361 gatgcagaat ttatctcaga tatggcaata ctcattgata gagggctttg ttcaaagtcg
      421 gcacctgcaa ccgaagctat ttataaaaaa tacgatgatg attttccttt agagaatatc
      481 tataacgaga gaattgatta tttcttcaat ttattggcgg aagatttttc cgaattacgt
      541 aattcattta tgatgaaatc gtatgttgtt cactcattat tttgtgctat taccgcatta
      601 aaatttggtt tccccggaag tgatgaactt gaaaatgaac tcggtttcac ggctaaccca
      661 aactttgatg taaatccttc ccgtgtaatt ccgattctct tagagatggc tgatgcccat
      721 gaaattcagg acgaagatgg cccttatgca gcctatgtaa caagttgttt atcatcaaca
      781 accaagctac cacaacgaac cacgcgcaca aaagaactga ttaaagcatt cttatgaggt
      841 ttgatatgga caaaatttat cgaaatttta ttcataggtg cagcgtatat caaggcaaag
      901 ttgatattct tcgttttaat aggattgaat atgatgagtg gacacatgcc aaattgactg
      961 aagggttgat gtctgattta tggcaaaatt ggtgtctatt ctgtagaaat ctcatccata
     1021 aatcttgtag aggtgcagtt tgtgctgata ctaccgtttt cggcccaaga gtgacccagt
     1081 atggaaactc ctggcaacga cttggatatg aagcaaaaaa tataaacggc acaataaaaa
     1141 ataatggaca taacagtttt cttatcagat atgaaccaac atggggagat attgacaaga
     1201 tagtaaatat aatcacccat cttaatccta aaaataagaa ttgtttgtta gctgcatttg
     1261 gtatggggct taaggatatt aactacctac aacttgcaag aaatgcatgt gcacataaaa
     1321 acgtcgagac aattgaggag ctgattaaca aaacaaagct tttttatgac gtaactactt
     1381 taaaaaaacc cagtgatttc gcatggtctc ttaaaaaggg gactcatagt tttgcattct
     1441 atacttggtt atatgaaatg agaatcattg ctaaaaatgc catattgact tgttaaacat
     1501 ccaattattc agcataaagt tttgataact tattttgata aaattttgat aaccgttagc
     1561 agcctaataa aaaacaacgg gagcgtaaca ctcccgttaa tgaatttaac aaattaccgt
     1621 attacatgtt cttgatgatc gcatcaccaa actctgaaca tttcagcagc ttagcgcctt
     1681 ccatcagacg ttcgaagtca taggttacgg tcttggcgtt gattgcacct tccatacctt
     1741 taacaatcag gtcagcggct tcagtccaac ccatatggcg taacatcatc tcagcggaga
     1801 gaataataga gccagggttt actttgtcct gaccggcgta tttcggcgca gtaccgtggg
     1861 tggcttcaaa cagggcgcat tcgtcaccga tgtttgcacc aggggcgata ccgataccgc
     1921 caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata caggcgataa
     1981 catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca atcacgtctt
     2041 taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg ccgccgtcga
     2101 tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta aacgcgcctt
     2161 cggtgaactt catgatgttg cctttgtgca ccagggtcac agagtcacgg tcgttggcaa
     2221 ttgcgtattc aatcgcggca cggaccagac gtttggtgcc ttcttcagaa cacggcttga
     2281 taccgatacc gcaatgttcc gggaagcgaa ttttcttcac gcccatctct tcacgcagga
     2341 atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc gcataaatgt
     2401 cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta accgggcttg
     2461 gagtgccttg atagtaacgt accggacgca ggcagatgta gagatccagt tcctggcgca
     2521 gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga cctttaatgg
     2581 caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca tcctgaccat
     2641 aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta cgctcgcctt
     2701 tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt acatctacac
     2761 cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg agtttgccgt
     2821 tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc attcacctct
     2881 ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc
     2941 gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg tcaatactac
     3001 cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc aattcataat
     3061 ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc gattcagctc
     3121 gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc tgttcaataa
     3181 accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat taaaagaatt
     3241 catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg aagggttact
     3301 ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggta aacgcaccgg
     3361 gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg aagccttgcg
     3421 caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc tggttgacga
     3481 accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta ttcccaccag
     3541 ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca tgaccgccca
     3601 tgttggtttc cccacgctgc gactgattcg ctatgcgatg ggagattact ctttggataa
     3661 tcttgccaat ggcgaatggc gagaagtgac agattaagga tgcaaaatgt ttaaaccgca
     3721 cgttaccgtt gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg aagagacgat
     3781 taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg aaaccttagt
     3841 ggaagccgcc gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc cgcaacactt
     3901 tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt tcctctttgc
     3961 cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg actgctgtcg
     4021 ttgggtcagc gccgaagaga ttttaaaggc gtcaaatctt cgttcgccgc tggtggcgga
     4081 aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg gcgattttaa
     4141 ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc cgccttgaag
     4201 ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt cggcatatcc
     4261 ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata tcaggttgaa
     4321 ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac agcggcagcg
     4381 gatctggctg atgcccaggc tgtctgcgac aagctcggca ttgaactgca caccgttaac
     4441 tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata taaagccggt
     4501 cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc cttcctcgaa
     4561 tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt acgtcgggcc
     4621 gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga ccagagctac
     4681 ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt cggcgaactg
     4741 gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc gaagaaaaaa
     4801 gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct gggccgttat
     4861 ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg tgagcaccag
     4921 gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg caccaaagaa
     4981 ggtaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat tctggttgtc
     5041 gctcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca gcagttgcac
     5101 tgggtcgatc gcgaaccatt caccggcact atgcgttgca cggtaaaaac ccgctatcgc
     5161 cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga agtgattttc
     5221 gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa cggtgaagtg
     5281 tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt attatcttta
     5341 cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc ctcgccctgg
     5401 ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg cattgtgatg
     5461 ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc tcgacgctgg
     5521 cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg ctcggcgtgc
     5581 tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca ctcagcttga
     5641 tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg ggcaaccgga
     5701 tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg ctgatgagcg
     5761 cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt caggtcaccg
     5821 gttcccctgc tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca accctgctgg
     5881 caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg caactgatgt
     5941 tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta accccggagt
     6001 tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga cgctacggcg
     6061 ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa ttccgtgtac
     6121 aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag gaagttcctg
     6181 cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc agcgaagaag
     6241 atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa gcggttgagt
     6301 atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct gaattcatcc
     6361 actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg ctgaaaaccg
     6421 cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt aaagatctcg
     6481 ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca gccacgccgt
     6541 caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag taccgccagc
     6601 ttaatcaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat aacgcccata
     6661 tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc acctcgctgg
     6721 gtattcagtg gaacccgtac accacccaga tcgaaccgca cgactacatt gccgaattgt
     6781 ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac gtctggggtt
     6841 atatcgccct taaccacttc caacagaaaa ccattgctgg tgagattggt tcttccacca
     6901 tgccgcataa agttaacccg attgacttcg aaaactccga agggaatctg ggcctttcca
     6961 acgcggtatt gcagcatctg gcaagcaaac tgccggtttc ccgctggcag cgtgacctga
     7021 ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg attgcgtatc
     7081 aatccaccct gaaaggcgtg agcaaactgg aagtaaaccg tgaccatctg ctggatgagc
     7141 tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt cgctatggca
     7201 tcgaaaaacc gtacgagaag ctgaaagagc tgactcgcgg taagcgcgtt gacgccgaag
     7261 gcatgaagca gtttatcgat ggtctggcgt tgccagaaga agagaaagcc cgcctgaaag
     7321 cgatgacgcc ggctaactat attggtcgag ctatcaccat ggttgatgag ctgaaataaa
     7381 ctcgtatcag tgccggatgg cgatgctgtc cggcctgttt attaaaatta tccacttttt
     7441 cttttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc cataaacaca
     7501 taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc gcgtactggt
     7561 tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg atgctggtca
     7621 tcaggtcgat gacgcagaag atgccaaaga agccgattat tatctcaatg aacatttacc
     7681 ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac tgattcgccg
     7741 ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg aaagctggca
     7801 ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac catttcatat
     7861 tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc tggcttcaca
     7921 ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat ctattaatga
     7981 cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga tacgcaataa
     8041 tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg cggagctgcg
     8101 ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc aggcacaata
     8161 tccgcaagaa gtcattacca ccgttcgcgg ccagggctac ctgttcgaat tgcgctgatg
     8221 aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct gttggcaacg
     8281 gcagcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc
     8341 gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct gttctatacc
     8401 cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat cgacaagcaa
     8461 agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc gcaacgtgac
     8521 gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg ttttcatgaa
     8581 attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc gatacagcaa
     8641 cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc ggtggcagta
     8701 aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt ggataccatt
     8761 ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt gctctcagcc
     8821 aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt acgccccatc
     8881 gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt gctcaatcca
     8941 gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt aaaaagtgaa
     9001 cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg
     9061 ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt
     9121 gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat tggctactac
     9181 ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc
     9241 ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat
     9301 atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga ttttgtcgag
     9361 gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt cgaaatttct
     9421 gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc cggtattcca
     9481 ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt acgccctggg
     9541 caaggtgtag ggctggcggt agtccgcgaa atcaccgagc aatatgaggg taaaatcgtc
     9601 gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg ccagcattct
     9661 gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca tccgttataa
     9721 tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac tcttaactgg
     9781 cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg cggctttaat
     9841 aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga aagcgaagtt
     9901 gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg cccgttcgaa
     9961 agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt gaaccactgg
    10021 catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg gcgtattgat
    10081 gatctgatga tctctttttc tgtaccgggc ggcggcgtcg gcccgcatct cgatcagtac
    10141 gacgtgttta tcattcaggg taccggtcgt cgtcgctggc gagtgggcga aaagctgcaa
    10201 atgaaacagc actgtccgca cccggatctg ttacaggtcg atccgttcga agccatcatc
    10261 gatgaagagc tggagcctgg cgatattctt tatattccgc caggattccc gcatgaaggc
    10321 tacgcgctgg aaaatgcgat gaactattcc gtgggttttc gcgcgccaaa tacgcgggaa
    10381 ttaattagcg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa ctactacagc
    10441 gatccggatg ttccacctcg cgctcatcct gcggacgttc tgccgcaaga gatggataaa
    10501 ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca atggtttggc
    10561 gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc gccttatcag
    10621 ccagatgaaa tctacgatgc gctgaaacaa ggtgaagtgc tggtgcgcct gggtggtctg
    10681 cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga ttccccgcac
    10741 cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa ttttggcgat
    10801 gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag cgggtattgg
    10861 ttcttcgaag ggtaagtttg gatttaagcc ggatgcggcg ctgattgcgc cttatcgtgc
    10921 ctacatgcac tgaacgcctg taggcacgat aagtatgttg accagtatca atataacggt
    10981 ttcatcccgg taattggcag tccacatacg catcgggcaa tcacgacttc cgttgcgccg
    11041 ttaactcggc aatacggaca atcacctgca ccgctttttc cataccttcc agagtcacaa
    11101 actcatgctt accatgatag ttgtaaccgc cagtgaacag gttcgggcac ggtaatccca
    11161 taaacgacaa ctgcgcgccg tcggtaccgc cgcggatcgg tttcagttcc ggttcaatat
    11221 cgcagtcacg catcgcctgc tgggcgatat cgataatatg cggatgctca accactttct
    11281 cgcgcatatt gtagtaactg tcttcaatca ccagttcaat gtagcaatca ggatgtaacc
    11341 ctttgcccac ttttttggcg atctccatca ttttacgttt acgcgcttca aactgtttac
    11401 ggtcgaaatc acggatgatg tagtgcatat cggctcggtc aacggtgcct ttcatgctcg
    11461 ccaggtgata gaaaccttca tagccttctg tcatttccgg gctttcatcc gccggaactt
    11521 ccgcatgaat acgtgccgcc agcgacagcg catttaccat cactcctttc gccgtgcccg
    11581 gatgaacatt gttaccgaca attttgatat tgaccgatgc ggcgttgaag ttttcaaact
    11641 ccagttcgcc tacgccacca ccgtcaacag tgtaagccca gcgggcatcg aaggcatcaa
    11701 catcaaaatg tttcgcccct ttgcccactt cttcatccgg ggtaaaggcg acgcgaatat
    11761 caccatgcgg aatgtttttc tgttgcaata ccgccagcgc ggtcatgatt tctgcaatac
    11821 cggctttgtc atcggcacct aacaaggttt taccatcggt agtaatcagc gtctgaccca
    11881 gtagctgatg cagcaccggg aacataaccg gtgataaaac ttcatcgccg atacccagcg
    11941 caatatcgcc accgcgatag ttttcaacaa tttgcggatt cacatttttg ccgctgcaat
    12001 ccggtgaggt atccacatga gaaataaagc caatcgccgg gatattgcca gggacgttag
    12061 ccggtaacgt cgccatcaaa gtgcccttct cacttaaggt cacattgata agccccatct
    12121 cttcgagctg ctctttcagc agatgcaata acttccattg gccttccgtg ctgggaacct
    12181 gtctcacccc tgcttttgat tgggtatcca gagacacgta gttcaaaaat cgctcaagta
    12241 gtttatccat gtagtcaccc tcactttttg tgacaacatt attaagaagc aagaaaagac
    12301 aaatattgcg tcaggtcact tttacccctg caagcgggaa tatttatcag cattacctta
    12361 atgaatataa agctaagcca gtaattcaca acaaagattg gcgattcaag cggtttgccg
    12421 tagaattacc gccctcatta agagtcacca aggtggttaa ccacaaaccc cgcatcggta
    12481 agccatccgt tgcgtttaca tgggacagag taaaaaattg aataaacaac cgagttcgct
    12541 ttcaccgctg gtgcaattgg cgggaattcg caaatgcttt gatggtaaag aggtcattcc
    12601 ccagctggat ctgactatca acaatggcga gttcctcacg ctgcttggcc cttctggctg
    12661 cggtaaaaca accgttcttc gcctgattgc aggtctggaa actgttgatt ccggacgcat
    12721 catgctggat aacgaggaca tcacccacgt tccggcggaa aaccgctatg tgaacactgt
    12781 tttccaaagc tacgcacttt tcccccacat gaccgtgttc gaaaatgtgg cctttgggtt
    12841 gcgcatgcaa aaaacccccg ctgctgaaat tacgccccgc gtgatggaag ccctgcggat
    12901 ggtgcagctg gaaaccttcg ctcaacgcaa accgcatcag ctctctggtg gtcaacagca
    12961 acgcgtcgcc attgcccgcg cggtggttaa caagcctcgt ctgttgttgc tggatgagtc
    13021 gctctcagcg ctggattaca aactgcgtaa gcaaatgcag aacgagctga aagcgttaca
    13081 gcgtaagctt ggcattacat tcgtcttcgt gactcacgac caggaagaag cactcaccat
    13141 gtcggacagg attgtggtga tgcgcgatgg tcgcattgag caagacggca cgccgcgtga
    13201 aatctacgaa gagccgaaaa acctgtttgt tgccggcttc attggcgaaa tcaatatgtt
    13261 taacgccacg gtcatcgaac gtctcgacga gcagcgcgta cgcgccaacg ttgaaggccg
    13321 cgaatgtaat atctacgtta acttcgccgt tgaaccgggg caaaaactgc atgttctgct
    13381 gcgcccggaa gacttacgtg ttgaagagat taacgacgac aaccacgctg aagggctgat
    13441 tggttacgtt cgcgagcgta actacaaagg catgacgctg gagtcggttg ttgaacttga
    13501 aaatggcaag atggtgatgg tcagcgaatt cttcaatgaa gacgaccctg actttgacca
    13561 ctctctcgac caaaaaatgg ccattaattg ggtagaaagc tgggaggtcg tactggctga
    13621 tgaagaacac aagtaagttc cagaatgtag tgattgtcac tattgtcggt tggcttgtgt
    13681 tgtttgtctt tctgcccaac ctgatgatca ttggcaccag ctttttgacc cgcgacgacg
    13741 ccagtttcgt caaaatggtc tttacgctgg ataactacac gcgtctgctc gatccgctct
    13801 attttgaagt gctattgcac tcgctgaata tggcgctgat cgccaccctc gcctgcctgg
    13861 tgctgggcta cccgtttgcc tggtttctgg cgaagctgcc acacaaggtg cgtccgctgc
    13921 tgctgtttct gctgattgtt cccttctgga ccaactcatt gattcgtatc tacgggctga
    13981 aaattttcct cagcaccaaa ggctatctca acgagttttt gctctggctg ggcgttatcg
    14041 acacaccaat tcgtatcatg ttcacgccca gtgcggtgat tatcggtctg gtttacattc
    14101 tgctaccgtt tatggtgatg ccgctgtact ccagtatcga aaaactggat aagccgttgc
    14161 ttgaggcggc gcgcgatctc ggtgccagca agttacagac ttttatccgt atcattattc
    14221 cactgacgat gccgggaatt attgccggat gtctgctggt gatgctgcca gcgatgggcc
    14281 tgttctatgt atccgacctg atgggcggtg cgaaaaacct gctgatcggt aacgtcatca
    14341 aggtccagtt cctcaatatt cgtgactggc cgtttggtgc agccaccagc attacgctga
    14401 ctatcgtaat gggcctgatg ttgctggttt actggcgcgc ttctcgtttg ctgaataaga
    14461 aggtggaact cgaatgatcg gtcgactgct tcgcggcggt tttatgaccg ctatctacgc
    14521 gtacctgtat atcccaatca ttattttgat tgtgaactcc tttaacagct cgcgctttgg
    14581 catcaactgg cagggtttta ccaccaaatg gtatagcctg ctgatgaaca acgacagcct
    14641 gttacaggca gcacagcatt cactaacaat ggcggtgttt tcggcgacgt ttgctacgct
    14701 tatcggttca ctgacggcag ttgcgctgta ccgttatcgc tttcgcggta agccgttcgt
    14761 tagcggaatg ctgtttgtgg tgatgatgtc gccagatatt gtgatggcga tttctctgct
    14821 ggtgctgttt atgctgctgg gtattcagct tggcttctgg tcgctgctgt tctcgcatat
    14881 caccttctgc ctgccgtttg tggtggtgac ggtgtattcg cgcctgaaag gttttgacgt
    14941 gcggatgctg gaagcggcga aagatctcgg tgccagtgaa tttaccattc tgcgaaaaat
    15001 cattctgcca ctggcaatgc ctgcggtggc ggcgggctgg gtgttaagct ttaccctgtc
    15061 gatggacgat gtagtagttt cttcgttcgt caccggacca agctatgaaa ttctgccgtt
    15121 aaaaatttat tcgatggtca aagtcggcgt atcgccggaa gttaacgcgc tggcaaccat
    15181 attactggtg ctgtcgctgg tgatggtaat tgccagccag cttattgctc gtgataaaac
    15241 gaaaggtaac acaggggacg ttaaatgaaa aaatggtcac gccacctgct cgcggcgggt
    15301 gctctggcac tgggcatgag cgccgctcac gccgatgaca acaatacgct gtatttctac
    15361 aactggaccg agtacgtgcc gccaggactg cttgaacagt tcaccaaaga aaccggtatt
    15421 aaggttatct attcgactta cgagtcgaac gaaaccatgt acgccaagtt gaaaacgtac
    15481 aaagacggtg cctacgacct ggtggttcct tcaacctatt acgtcgataa aatgcgtaaa
    15541 gaagggatga tccagaagat cgacaagtcg aagttaagca atttcagcaa cctcgatcca
    15601 gacatgctca acaagccgtt tgacccgaat aacgactact ccattccgta tatctggggg
    15661 gcgacggcga ttggcgttaa cggtgatgcg gtagatccga aatccgttac cagctgggcc
    15721 gatctgtgga agcctgagta caaaggtagc ctgctgctga ccgacgatgc ccgtgaagtg
    15781 ttccagatgg cgctgcgcaa actgggctac tccggtaaca ctaccgatcc gaaagagatt
    15841 gaagctgcat ataacgagct gaaaaaactg atgccaaacg tcgcagcgtt taactccgat
    15901 aacccggcta acccgtacat ggaaggcgaa gttaacctcg gcatgatctg gaacggctct
    15961 gctttcgttg cacgccaggc gggtacgcca attgacgtgg tgtggccgaa agaaggcggc
    16021 atattctgga tggacagcct ggcgatcccg gcaaatgcca aaaacaaaga aggcgcgctg
    16081 aaattgatca acttcctgct gcgcccggat gtggcaaaac aggttgctga aactatcggt
    16141 tatccaacgc caaaccttgc ggcgcgtaag ctgttaagtc cagaagtggc gaacgataaa
    16201 acgctctacc ccgatgctga aaccattaaa aatggcgaat ggcagaatga cgttggcgca
    16261 gccagcagca tttatgaaga gtattatcag aagctgaaag caggacgtta attcagcaag
    16321 cgggcggtgg tattaccgcc cgttttttag ttagcaagga tgtggctaat gagtcaggac
    16381 agtaaagtgt tatcccgcgc ttttctcggt atcggtctga tattgctcgt tatttctgca
    16441 atcatctttt attaccattt tacttttacc aaaagcgcgg ttcatacaga aggaatcatt
    16501 gttgatgccg tttggtacaa caatcactca aacgacgtcg atgataatgg ttcctggtat
    16561 ccagtggttg cctttcgccc tacgccagat tacacgctaa ttttcaattc gaatatcggt
    16621 agtgattttt atgaagatag cgaaggtgac agagtcaatg tctattatcc gccagggcat
    16681 ccagaacaag ccgaaataaa caatccctgg gttaattttt ttaaatgggg ctttgttggc
    16741 atagcaggca ttattttcgg tagtgtcggt ctgataatca cgctcccctc caccaaaaaa
    16801 tctcgcagga aacggaagtc ccgcccatga aaaaattcat tatactgctc agtttactga
    16861 tactcctgcc tctggtagca accagtaaac cattgattcc aataatgaag acacttttta
    16921 cggatgttac cggtactgta ccggatgctg aagagatcgc acgtaaagcc gagctttttc
    16981 gccaacaaac gggaattgca ccgtttattg tgattttacc cgacattaat aatgaagcca
    17041 gtctcagaca aaatggtaaa gcgatgctgg cgcatgcgtc atcttcattg agtaatgtaa
    17101 aaggaagtgt tctgttacta tttactaccc gcgaaccacg gttaattatg atcaccaacg
    17161 gccaggttga aagtggtctg gacgataaac atctcggcct tctgatagaa aatcacacgc
    17221 tggcttattt aaatgcagat ctctggtatc agggaatcaa taatgcattg gctgttctac
    17281 aagcacagat attaaaacaa ccgacgccgc cactgacgta ttatccgcat ccaggacaac
    17341 agcatgaaaa tgctcccccc ggcagcacca acacattagg ttttatcgcc tgggctgcga
    17401 cgtttatctt attttcctgg atcttttgct acacgactcg ttttatttat gcgttaaaat
    17461 ttgcagtggc gatgactatt gccaatatgg gttatcaggc tttatgtttg tatatcgata
    17521 atagctttgc gattaccaga atatcgccac tctgggcagg gctaattggt gtctgtacgt
    17581 ttattgccgc attgttattg acgagtaaac gataaaagag gccggaaaag tgcgcgtgta
    17641 ttatgccgga tggggcgcga acgccttatc cggcccacaa aacccgcaaa ttcaattaat
    17701 tgcgtcccct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat
    17761 cccttcagca acttttcaac aaactcaggc accacctggc ttgccgggcc gtaatatttc
    17821 tcggcaaatt cattaccgac ctgacttggt tcgagattca gttccacggt gtgcgcgcca
    17881 tgcagtttcg cttcgtgaac aaacccagcc gccggataaa catgcccgga agtaccaatg
    17941 gcaatgaaaa tatcagccat cgacaacgcc atataaattt catccatgcc gagtggcatt
    18001 tcgccaaacc aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta
    18061 tcttctggcg taacgtctcc ggtccagtcg agaacctgac cattttgcga acaacgaact
    18121 ttcagcaatt ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg
    18181 tctatattct gcgttaccag caaaaagcga tcgccgaggg cgtcttgcag tttagccagc
    18241 gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta
    18301 taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca
    18361 acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca
    18421 gaaatccctg cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
    18481 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
    18541 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt
    18601 agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg
    18661 cgcggaacac gagctacagg taagagatga cgaggcagcc tgtccgccag ttgcgttgtg
    18721 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
    18781 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
    18841 tgctcatcgc cttgatcata aagcgcaata atttcgggag cctgcaacgg ttgatgataa
    18901 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
    18961 tgaccacagc cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc
    19021 ggcagacgca tatggccaaa ctcgccggta atgtagcttt taccggtaat cggtttgccg
    19081 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac cagtggatat
    19141 tgagtaaatt catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg
    19201 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagcg
    19261 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
    19321 ccgacagaac ctttacagcc aaaacgttgg tcagcttcag ctaccagctc acacactgca
    19381 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
    19441 cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac
    19501 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
    19561 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
    19621 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaaat caataaaata
    19681 gatatcgctg gagaggaact gatgaccgat caacttttca atccactcaa taatcggggt
    19741 aagttgcagt gaaaccacca caccgataat caccccacac aggctgccga acagccctgc
    19801 cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct
    19861 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
    19921 gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
    19981 gatatcgcga tacatatagc cgtaagtacc aatccaactt ttaatataaa catagctgtt
    20041 ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
    20101 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat
    20161 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
    20221 cacacgtttt ggctgcatca gtttatgctc gggattcgag ttggggatca taatcgacac
    20281 ccaatccccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg
    20341 ttcgcccgct ttaaaattgc gccacgcatc gccctggaca aacgagggta atgcgctcag
    20401 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgaagat tcacgccact
    20461 ttccaccagc ccggtgaaat tgatatacgg cgcggcagcg gcaatacccg gcactttttg
    20521 cacgtgatcc agtgcttcct gccagttagt ccacggctga tccaccgcct cgatttcacc
    20581 atgcggcacc accgccagaa tacggttgtt cagttcgcgt tcaaagccgt tcatcgcgct
    20641 taagccgacg atcaataccg ccacgccgag ggcaatgcca atggtagaaa taacggagat
    20701 cagcgacacc atgccgccgc gccgccgtcc gcgactaaaa cgcaggccaa ttaataacga
    20761 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
    20821 cgcatttcta actggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
    20881 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtcggcgtta
    20941 cgcgcatcga ggttaccggt aggttcatcc gccagtacca ggcgcgggtt attcaccagc
    21001 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
    21061 gcacgatgat ccagccccac cgcttttaac atctcaagtg cacggctgtt gatttcagcg
    21121 ggctttttct tgccaatcag cagcggcata gccacgtttt ccagggcggt aaaatccggc
    21181 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagctc tgctttcgcc
    21241 gccgaagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggcgta
    21301 tccagcccgc ccaacaagtg cagcaaggtg cttttaccgg aaccagagct accgacgatc
    21361 gccatcattt caccttcgcc gacgctgaaa ctgacattgt gcagcacatc ggtttgcaca
    21421 ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg
    21481 taaagcctca gcgggttgag tggcggcagc gcgccatgaa gggtaaagcg tagacagcag
    21541 cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga tagcgaccgg
    21601 cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc
    21661 aagcagggcg ccaagcgccg ctccgaggat cgcaccgata atcccggcgc tggccccttg
    21721 caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac
    21781 ttcgccctgc ttctccatta ccatcagccc tagtgaggta ataatgttaa acgccgcaac
    21841 ggcgacaatc aggctcagca gcaagcccat catatttttt tccatgcgta cggcctggaa
    21901 cagctcgcct ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg
    21961 acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg taatattgcc
    22021 tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt cattgccatc
    22081 gacttcactg ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg
    22141 ccccatcggc gtgaactggc tggcagatgg taccatcatg cggatttgat caccgcgatt
    22201 aacgcctagc tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag
    22261 gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg ccgggtcgat
    22321 accgagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt
    22381 aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg
    22441 gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt
    22501 ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc
    22561 gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccgctgcacg
    22621 cccacgcatg taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg
    22681 gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg
    22741 taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccatg
    22801 ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata
    22861 cccattacac ctcgggtcat aacccgcagg atcaaccgca tgtaaattaa aaaagagcgt
    22921 gttgtcctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt
    22981 ctggtggtga tttatcactc ggtcattacc ttttatccgc atctgaccac ttttcagcat
    23041 ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct tgcccccttt
    23101 cgtatgccgg tttttttctt tatttccggc tatttgattc gccgctatat cgacagcgta
    23161 ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg
    23221 ggcgtggtgc agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat
    23281 ttaagcaatg cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg
    23341 atcaccgcca gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt
    23401 aaaattttta gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg
    23461 gttaatttcg ttcccacgcc gtggtgggga atgaacagtg tgatccgcaa tttgctttat
    23521 tacagccttg gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg
    23581 cgccgccatc tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt
    23641 actatctcgc tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcaa
    23701 tacgagcaac gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc
    23761 attgctatct acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg
    23821 caaatgaacg cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc
    23881 tttgtcagtt tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt
    23941 gcattcagcg atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactctcgt
    24001 taaaccatcg ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg
    24061 aacagcattg cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct
    24121 gacaaccgtt atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg
    24181 tctgctgggc gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg
    24241 tcacgccggt ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga
    24301 tgaaatcagc cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc
    24361 ctacgacagt ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca
    24421 gctaccgacg atgcagcgtg gcgtactgat tgttccggtg aatacgctta tgcagcgcgt
    24481 ttgcccacac agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc
    24541 acgagatgca ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat
    24601 ggagcacggc gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga
    24661 gctgccttat cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt
    24721 cgacagccag cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt
    24781 tccgaccgat aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt
    24841 gaagcgcgat ccggaacata tttaccagca agtgagtaaa ggcacattac ctgccgggat
    24901 cgagtactgg cagccattgt tcttcagcga accactgccg ccgctgttca gttatttccc
    24961 tgccaatacc ttgctcgtga atactggcga tctggaaacc agtgccgaac gtttccaggc
    25021 tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc
    25081 acaatcgctc tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgggt
    25141 gcagctaaaa actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa
    25201 actgccagac ctggccgttc aggcacaaca aaaagcgccg ctggatgcgc tgcgtaagtt
    25261 cctcgagact ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc
    25321 gctgggtgaa ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga
    25381 agccagcgac cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa
    25441 ggtgcgtaat ctggctctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg
    25501 tcgtcaggat tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct
    25561 gcatattggt cagccggtgg tccatctgga gcacggtgtc gggcgctatg ccggaatgac
    25621 cacgctcgaa gcgggcggca ttactggcga gtatttgatg ctcacctatg ccaacgacgc
    25681 caaactgtat gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga
    25741 agaaaacgcc ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc
    25801 ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc
    25861 caaagagggc ttcgcgttta aacacgatcg tgagcagtat cagttgttct gcgacagctt
    25921 cccgtttgaa accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg
    25981 tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt
    26041 ggcgatgcgc gccgctttcc tggcagtaga taaccacaag caggtggcgg tgttggtgcc
    26101 taccaccctt ctcgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc
    26161 ggtacgtatc gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc
    26221 ggaagtggcg gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga
    26281 cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg
    26341 tcataaagag cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac
    26401 gccgatccca cgcacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc
    26461 cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt
    26521 ggtccgggag gcgatcctgc gtgaaatttt gcgcgggggg caggtttatt atctctacaa
    26581 tgatgtggaa aacatccaga aagctgccga acggctggca gaactggtgc ctgaagcacg
    26641 gattgccatc ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt
    26701 ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt atcgaaaccg ggatcgacat
    26761 cccgacagcc aacaccatta tcattgaacg cgcggatcac ttcggtctgg cgcagctgca
    26821 ccagttacgc ggtcgcgtcg gacgttcaca tcatcaggca tatgcatggc tgctgacgcc
    26881 gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga
    26941 agatctcggt gcaggctttg cgctggcaac gcacgatctg gagatccgcg gcgcgggtga
    27001 actgcttggc gaagaacaaa gtggctcaat ggaaaccatc ggtttctcgc tgtatatgga
    27061 gttgctggaa aacgccgtcg atgcactgaa atccggacgc gagccgtcgc tggaagatct
    27121 caccagccag caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat
    27181 tcctgacgtg aatacgcgtt tgtcgttcta taaacgtatt gccagcgcca aaacggaaaa
    27241 cgaactggaa gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cggatccggc
    27301 gcgtaccctg ctggatgttg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa
    27361 gctggaaggt aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc
    27421 ggcctggttg attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac
    27481 gcgcctgaaa tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca
    27541 gtttatgcgt gaactggaag agaacgcgat cgcttaatgg taaatcgggg gcgtttctgc
    27601 gcccccatct gcaacattta caaattcttt gcacttccct gcactatccg acactgtcac
    27661 catccataat tcaggcttat ctgtttatta caataacctt atatttatta tggatttttg
    27721 gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg
    27781 gcgctggcgc taccggcaaa agccaacacc tggccgctgc cgccagcggg cagtcgtctg
    27841 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    27901 aaaaaatata acgtcggctt tctcgctctg ttacaggcta accctggcgt tgatccttac
    27961 gtaccgcgcg cgggtagcgt gttaacgatc ccgttgcaaa ccctacttcc agatgcgccg
    28021 cgcgaaggca ttgtgatcaa tattgcggag ctgcgtctct attactaccc gccgggtaaa
    28081 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    28141 acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac
    28201 atccgcgccc gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggaccg
    28261 gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    28321 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    28381 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    28441 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    28501 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    28561 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    28621 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg
    28681 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    28741 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    28801 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    28861 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgatt gtaccgactt
    28921 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagctggca aatgacatgg
    28981 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    29041 gatgaatgac gtcgcaagca attacttaac gatcgataac taaatgatag at
//
LOCUS       DAZFFI010000053        28572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFFI010000053 DAZFFI010000000
VERSION     DAZFFI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.053"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1273
                     /locus_tag="ACHJ12_002712"
     CDS             <1..1273
                     /locus_tag="ACHJ12_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4990477.1"
                     /translation="GCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWKI
                     SQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATG
                     HLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQP
                     YLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCN
                     GNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPITV
                     SIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEA
                     LLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAG
                     PEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1463..1576
                     /gene="yhiY"
                     /locus_tag="ACHJ12_002713"
     CDS             1463..1576
                     /gene="yhiY"
                     /locus_tag="ACHJ12_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4990478.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1639..2706
                     /gene="yhiI"
                     /locus_tag="ACHJ12_002714"
     CDS             1639..2706
                     /gene="yhiI"
                     /locus_tag="ACHJ12_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4990479.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2703..5438
                     /gene="rbbA"
                     /locus_tag="ACHJ12_002715"
     CDS             2703..5438
                     /gene="rbbA"
                     /locus_tag="ACHJ12_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4990480.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPELLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYISLGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5438..6562
                     /gene="yhhJ"
                     /locus_tag="ACHJ12_002716"
     CDS             5438..6562
                     /gene="yhhJ"
                     /locus_tag="ACHJ12_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4990481.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6635..6910
                     /locus_tag="ACHJ12_002717"
     CDS             6635..6910
                     /locus_tag="ACHJ12_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4990482.1"
                     /translation="MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6907..7266
                     /locus_tag="ACHJ12_002718"
     CDS             6907..7266
                     /locus_tag="ACHJ12_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4990483.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7386..7787)
                     /gene="nikR"
                     /locus_tag="ACHJ12_002719"
     CDS             complement(7386..7787)
                     /gene="nikR"
                     /locus_tag="ACHJ12_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4990484.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7793..8599)
                     /gene="nikE"
                     /locus_tag="ACHJ12_002720"
     CDS             complement(7793..8599)
                     /gene="nikE"
                     /locus_tag="ACHJ12_002720"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4990485.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8596..9360)
                     /gene="nikD"
                     /locus_tag="ACHJ12_002721"
     CDS             complement(8596..9360)
                     /gene="nikD"
                     /locus_tag="ACHJ12_002721"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4990486.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9360..10193)
                     /gene="nikC"
                     /locus_tag="ACHJ12_002722"
     CDS             complement(9360..10193)
                     /gene="nikC"
                     /locus_tag="ACHJ12_002722"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4990487.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10190..11134)
                     /gene="nikB"
                     /locus_tag="ACHJ12_002723"
     CDS             complement(10190..11134)
                     /gene="nikB"
                     /locus_tag="ACHJ12_002723"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4990488.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11134..12708)
                     /gene="nikA"
                     /locus_tag="ACHJ12_002724"
     CDS             complement(11134..12708)
                     /gene="nikA"
                     /locus_tag="ACHJ12_002724"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4990489.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12819..13406)
                     /gene="acpT"
                     /locus_tag="ACHJ12_002725"
     CDS             complement(12819..13406)
                     /gene="acpT"
                     /locus_tag="ACHJ12_002725"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4990490.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13461..14510)
                     /gene="yhhT"
                     /locus_tag="ACHJ12_002726"
     CDS             complement(13461..14510)
                     /gene="yhhT"
                     /locus_tag="ACHJ12_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4990491.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14642..15859
                     /gene="yhhS"
                     /locus_tag="ACHJ12_002727"
     CDS             14642..15859
                     /gene="yhhS"
                     /locus_tag="ACHJ12_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4990492.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15863..16420)
                     /gene="dcrB"
                     /locus_tag="ACHJ12_002728"
     CDS             complement(15863..16420)
                     /gene="dcrB"
                     /locus_tag="ACHJ12_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4990493.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16493..17158)
                     /gene="yhhQ"
                     /locus_tag="ACHJ12_002729"
     CDS             complement(16493..17158)
                     /gene="yhhQ"
                     /locus_tag="ACHJ12_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4990494.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17379..17624
                     /gene="tusA"
                     /locus_tag="ACHJ12_002730"
     CDS             17379..17624
                     /gene="tusA"
                     /locus_tag="ACHJ12_002730"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4990495.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17726..19924)
                     /gene="zntA"
                     /locus_tag="ACHJ12_002731"
     CDS             complement(17726..19924)
                     /gene="zntA"
                     /locus_tag="ACHJ12_002731"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4990496.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAANRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19998..20624)
                     /gene="yhhN"
                     /locus_tag="ACHJ12_002732"
     CDS             complement(19998..20624)
                     /gene="yhhN"
                     /locus_tag="ACHJ12_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4990497.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20765..21124
                     /gene="yhhM"
                     /locus_tag="ACHJ12_002733"
     CDS             20765..21124
                     /gene="yhhM"
                     /locus_tag="ACHJ12_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4990498.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21127..21396)
                     /gene="yhhL"
                     /locus_tag="ACHJ12_002734"
     CDS             complement(21127..21396)
                     /gene="yhhL"
                     /locus_tag="ACHJ12_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4990499.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21386..21982)
                     /gene="rsmD"
                     /locus_tag="ACHJ12_002735"
     CDS             complement(21386..21982)
                     /gene="rsmD"
                     /locus_tag="ACHJ12_002735"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4990500.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22132..23625
                     /gene="ftsY"
                     /locus_tag="ACHJ12_002736"
     CDS             22132..23625
                     /gene="ftsY"
                     /locus_tag="ACHJ12_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4990501.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23628..24296
                     /gene="ftsE"
                     /locus_tag="ACHJ12_002737"
     CDS             23628..24296
                     /gene="ftsE"
                     /locus_tag="ACHJ12_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4990502.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24289..25347
                     /gene="ftsX"
                     /locus_tag="ACHJ12_002738"
     CDS             24289..25347
                     /gene="ftsX"
                     /locus_tag="ACHJ12_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4990503.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25592..26446
                     /gene="rpoH"
                     /locus_tag="ACHJ12_002739"
     CDS             25592..26446
                     /gene="rpoH"
                     /locus_tag="ACHJ12_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4990504.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26717..27820
                     /gene="livJ"
                     /locus_tag="ACHJ12_002740"
     CDS             26717..27820
                     /gene="livJ"
                     /locus_tag="ACHJ12_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4990505.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(28019..28402)
                     /gene="panM"
                     /locus_tag="ACHJ12_002741"
     CDS             complement(28019..28402)
                     /gene="panM"
                     /locus_tag="ACHJ12_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4990506.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTVSGTEGALDSLRVREVTRRRGVGQYLLEEVLRTNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6820 a         7375 c         7880 g         6497 t
ORIGIN      
        1 cggctgctat cttgataata ccgatattaa ttggtgcagc attgtttatt tacttaataa
       61 tatgacagta aatgatgatg ctaatgatgt tgcagttact gagtcgtaca aacccgtctg
      121 ggactggaaa atatcgcaat tcaatgtatc tgatattaaa ttcgaaacaa tgataaaccc
      181 ccagttcgct gacagaacct atttctccaa ttgttcacct gttgatccca caagtaccag
      241 accaacctat tttggcgata ccgatggctc agtcggtgca gttttgtacg cgctttttgc
      301 aacgggacat ttgagaatca tggctgaagg agaaaatttc cttagccagt tgcttaatat
      361 agaagatgaa gttctcaacg tattgctaag ggagaacttt aatgagcaac ttgacactaa
      421 tgttaataca ataatcagta ttctgaacag aagagatata gttttagaaa gtcttcagcc
      481 ttatttagtt attaataaag acgctgtgac gccatgtacg tttcttggcg accaaaccgg
      541 cgatcgattt agtaatatct gtggcgatca atttatcatc gatttgttaa aacgcataat
      601 gagtatcaat gaaaatgttc atgtattagc tggaaatcat gaaacgaatt gcaatggtaa
      661 ctatatgcag aacttcacgc gtatgaaacc acttgatgag gatacctacg ctgggataaa
      721 agattatcct gtatgtttct atgaccccaa atacaaaata atggcaaatc atcatgggat
      781 aacttttgat gagcagcgaa aacgttacat tataggaccg ataacagtct ctattgatga
      841 aatgaccaat gctctcgatc cggtcgaatt agcggcaatt atcaataaaa agcatcatgc
      901 tataattaat ggcaaaaagt ttaaaactag ccgggctatc tcttgtcgat cctttaatcg
      961 ttatttttct gtttcgacag attacagacc taaacttgaa gcactattag catgttcaca
     1021 aatgttaggt ataaatcaag ttgttgcaca taacggaaat ggtggaagag aacgcatagg
     1081 cgaaacgggg acagttttag gtcttaacgc ccgtgacagt aaacatgctg gaagaatgtt
     1141 cagcatgcat aattgccaaa ttaaccctgg tgcagggcct gagataacca ctccctggaa
     1201 gtcttaccag catgaaaaaa acagaaatgg acttatgcct ttgattagaa ggcgcacaat
     1261 gttacagctc tgaaccatag acaacatgaa tattaatgtt actatctggt tgagactgtt
     1321 gataaaacgt aaaaaggaat gctctgttcc tgcaactgaa caggcgtgaa acgctatcca
     1381 acaggatgga taccgttttg tcagccagtt ttatagatca cacttatgag tatgcgtgtt
     1441 tcatttacgg ccaggaatgc tgatgatgac aacattacta cccgttttca ctaagccttc
     1501 cccgttggcg ctcaatgctc tgcgcgctgg tcgtatttgc cgtttccttc ttatcccgga
     1561 tggaagaatc cgctgaacgt cgttcggcgg cagttgtaac acgcgtcgtt tgcttacagg
     1621 gattatggcg ggtcgattat ggataagagt aagcgccatc tggcgtggtg ggttgtcggg
     1681 ttactggcgg tggcggctat cgtggcgtgg tggctgttgc gcccggcagg tgtgccggaa
     1741 ggctttgctg tcagcaatgg gcgcattgaa gcgacggaag tggatatcgc cagcaaaatt
     1801 gccgggcgta ttgacaccat tctggtgaaa gaaggccagt ttgttcgaga aggtgaagtg
     1861 ctggcgaaga tggatactcg cgtgttgcag gaacagcgac tggaagccat cgcgcaaatc
     1921 aaagaagcac aaagcgccgt tgctgccgcg caggctttgc tggagcaacg ccaaagtgaa
     1981 actcgcgccg ctcagtcgct ggttaatcaa cggcaagctg aactggactc cgtagccaaa
     2041 cgtcataccc gttcccgctc gctcgcccat cgtggggcta tttctgcgca acagttggat
     2101 gacgaccgcg ccgccgctga gagcgcccga gctgcgctgg aatcggcaaa agctcaggtt
     2161 tcggcttcta aagccgctat agaagcggca cgcaccaata tcattcaggc gcaaactcgc
     2221 gtcgaagccg cacaagccac tgaacggcgc attgccgcag atatcgatga cagcgaactg
     2281 aaagccccgc gtgacggacg cgtgcagtat cgggttgccg agccaggcga agtgctggcg
     2341 gcaggcggtc gggtgctgaa tatggtcgat ctcagcgacg tctatatgac tttcttcctg
     2401 ccaaccgaac aggcgggcac gctgaaactg ggcggtgaag cccggctgat cctcgatgcc
     2461 gcgccagatc tgcgtattcc tgcaaccatc agttttgtcg ccagtgtcgc ccagttcacg
     2521 ccaaaaaccg tcgaaaccag cgatgaacgg ctgaaactga tgttccgcgt caaagcgcgt
     2581 atcccaccgg aattactcca gcagcatctg gaatatgtca aaaccggttt accgggcgta
     2641 gcgtgggtgc gggtgaatga agaacttccg tggcctgacg acctcgtggt gaggttgccg
     2701 caatgacgca tctggaactg gttcccgtcc cgcctgtcgc gcaactggcg ggcgtgagcc
     2761 agcattatgg aaaaaccgtt gcgctgaaca atatcacact cgatattccg gcccgttgca
     2821 tggtcgggct gattggcccg gacggcgtcg ggaagtcgag cttgttgtcg ttgatttccg
     2881 gtgcccgcgt cattgaacag ggcaatgtga tggtgctggg cggcgatatg cgcgacccga
     2941 agcatcgccg cgacgtctgc ccgcgcatcg cctggatgcc gcaggggctg ggcaaaaatc
     3001 tctaccacac cttatcggtg tatgaaaatg tcgatttttt cgctcgcttg ttcggtcacg
     3061 ataaagcgga gcgggaagtc cgaattaatg agctgctgac cagcaccggg ttagcaccgt
     3121 ttcgcgatcg tccggcaggt aaactctccg gcgggatgaa gcaaaaactg gggctgtgct
     3181 gcgcgttaat ccacgacccg gaactgttga tccttgatga gccaacaacg ggggttgacc
     3241 cgctctcccg cgcccagttc tgggatctga tcgacagtat tcgccagcgg cagagcaata
     3301 tgagcgtgct ggtcgccacc gcctatatgg aagaggccga acgcttcgac tggctggtag
     3361 cgatgaatgc cggagaagtg ctggcaactg gcagcgccga agagctgcgg cagcaaacgc
     3421 aaagcgccac gctggaagag gcgtttataa atctgttacc gcaagcgcaa cgccaggcgc
     3481 atcaggcggt agtgatccca ccgtatcaac ctgaaaatgc agagattgcc atcgaagcgc
     3541 gcgatctgac catgcgtttt ggttccttcg ttgccgttga tcacgtcaat ttccgcattc
     3601 cacgcgggga gatttttggt tttcttggat cgaacggctg cggtaaatcc accaccatga
     3661 aaatgctcac cggactgctg cccgccagcg aaggtgaggc gtggctgttt gggcaaccgg
     3721 ttgatccaaa agatatcgat acccgccgtc gggtgggcta tatgtcgcag gcgttttcgc
     3781 tctataacga actcaccgtg cggcaaaacc ttgagttaca tgcccgtttg tttcacatcc
     3841 cggaagcgga aattcccgcg cgagtggctg aaatgagcga gcgttttaag ctcaacgacg
     3901 ttgaagatgt tctgccggag ttattgccgc tcggcattcg ccagcggctt tcgctggcgg
     3961 tggcggtgat tcatcgcccg gagatgttaa tcctcgatga acctacttct ggcgtcgatc
     4021 cggtggcgag ggatatgttc tggcagttga tggtcgatct ctcgcgccag gacaaagtaa
     4081 ccatttttat ctccacccac tttatgaacg aagcggaacg ttgcgaccgc atctcactga
     4141 tgcacgccgg aaaagtgctt gccagcggta caccgcagga actggttgag aaacgcggag
     4201 ccgccagtct ggaagaggca tttatcgcct atttgcagga agcggcaggg cagagcaacg
     4261 aagccgaagc gccgcccgtg gtacacgaca ccacccacgc gccgcgtcag ggatttagcc
     4321 tgcgccgtct gtttagctac agccgccgcg aagcgctgga actgcgacgc gatccggtac
     4381 gttcgacgct ggcgctgatg ggaacggtga tcctgatgct gataatgggt tacggcatca
     4441 gtatggatgt ggaaaacctg cgctttgcgg tgctcgatcg cgaccagacc gtcagtagcc
     4501 aggcgtggac gctcaatctc tccggttccc gttactttat cgaacagccg ccgctcacca
     4561 gttatgacga gcttgatcgt cggatgcgtg cgggcgatat cacggtggcg attgagatcc
     4621 cgcccaattt cgggcgcgat atcgcgcgtg gtacgcctgt ggaactcggt gtctggatcg
     4681 acggagcgat gccgagccgt gccgaaacgg taaaaggtta cgtgcaggcc atgcaccaga
     4741 gctggttaca ggatgtggcg agccgacaat ccacacccgc cagccaaagc gggctgatga
     4801 atattgagac gcgctatcgc tataacccgg acgtaaaaag cctgccagcg attgttccgg
     4861 cggtgatccc gcttctgctg atgatgatcc cgtcaatgct aagcgccctt agcgtggtgc
     4921 gggaaaaaga gcttgggtcg attatcaacc tttacgtgac ccccaccacg cgtagtgaat
     4981 ttttgcttgg taaacagttg ccatacatct cgctggggat gctgaacttt ttcctgctct
     5041 gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg cagtttcctg acgctcaccc
     5101 tggcggcgct gctgtatatc atcattgcca ccggaatggg gctgctgatc tccaccttta
     5161 tgaaaagcca gattgccgcc attttcggaa cggcgattat cacgttgatc ccggcgacac
     5221 agttttccgg gatgatcgat ccagtagctt cgctggaagg gcctggacgt tggatcggcg
     5281 aggtttaccc gaccagtcat tttctgacta tcgcccgcgg gacgttctcg aaagcgctgg
     5341 atctgactga tttgtggcaa ctttttatcc cgttgctgat agccatcccg ctggtgatgg
     5401 gcttaagcat cctgctgctg aaaaaacagg agggatgatg cgccatttac gcaatatttt
     5461 taatctgggt atcaaagagt tgcgcagtct gctcggtgat aaagcgatgc tgacgctgat
     5521 tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc gttacgccag gatcgttgaa
     5581 cctcgcgccg atcgccattg ccgatatgga tcaatcgcag ttatcgaacc ggatcgttaa
     5641 cagcttctat cgcccgtggt ttttgccacc ggagatgatc accgccgatg agatggatgc
     5701 cggactggac gccgggcgtt atacctttgc gataaatatt ccgcctaatt ttcagcgtga
     5761 tgtcctcgcc ggacgccagc cggatattca ggtgaacgtc gatgccacgc gcatgagcca
     5821 ggcatttacc ggcaatgggt atatccagaa tattatcaac ggtgaagtga acagctttgt
     5881 cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg gaaacccgga tgcgctttaa
     5941 cccgaacctt gatcccgcgt ggtttggcgg ggtgatggcg atcatcaaca acattaccat
     6001 gctggcgatt gtattgaccg gatcggcgct gatccgcgag cgtgaacacg gcacggtgga
     6061 acacttgttg gtgatgccga taacgccgtt tgagatcatg atggcaaagg tctggtcgat
     6121 ggggctggtg gtgctggtgg tgtcgggatt atcactggtg ctgatggtga aaggcgtgct
     6181 gggtgtaccg attgaaggct cgatcccgct gtttatgctg ggcgtggcgc tcagtctgtt
     6241 tgccaccacg tcaatcggca tttttatggg gacgatagcg cgttcaatgc cgcaactggg
     6301 gctgctggtg attctggtgc tgctaccgtt gcaaatgctt tccggtggat ccacgccgcg
     6361 cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc atgccgacga cacactttgt
     6421 tagcctcgcg caggccatcc tctaccgggg tgccggattc gaaatcgtct ggccgcagtt
     6481 cctgacgctg atggcaattg gcggcgcgtt tttcaccatt gcactgctgc gattcaggaa
     6541 gacgattggg acaatggcgt aaggaaggga aacttgactt catactgtga tgtcatacta
     6601 ttgatgtcat aaagtgaagt cataaggaca tgccatgaga accaaggtac aggctttacg
     6661 gaagaaacaa aaaaatacat tggatcaaat agttaaaact cctgttcctc aagggatcaa
     6721 gtggtctgat atagagtcac tggttaaagc attaggcgga gaaattaagg aaggaagagg
     6781 ttcgcgttgc aagttcatac taaatatgag cgttgcgtgt ttccatcggc ctcatccgtc
     6841 gccagatacc gataaaggcg ctgtagaaag cgtgcgtgac tggttactaa gtataggggt
     6901 aaaaccatga tcaaactgaa gacgccaaac tctatggaga ttgccgggca acctgcagtt
     6961 atcacttacg tgccagaact gaatgcattt cgtggcaaat tcctgggctt gtcaggttat
     7021 tgtgattttg tctctgacag cattcagggg ctgcaaaaag aaggggaact ttccctgcga
     7081 gagtatcttg aggattgtaa agctgcgggt attgagccgt atgcgcgtac agaaaagatt
     7141 aagaccttta cgttacgcta tccagaatcg ctgagtgaac gccttaataa tgcggctgcg
     7201 cagcaacaag tctctgttaa tacctatatt attgaaacac tgaatgagcg tttgaatcat
     7261 ttataaattt tagcggcgta cattacgcag aatatctgcc tttacaggat ctatctatgc
     7321 aactgaactt aattttataa attaagttca gttaatatct tttctttcct cgtttcagaa
     7381 ataactcaat cttccttcgg caggcactgc aaatgcccgt gccgcacgcc gcgctgggcg
     7441 ataacgtcat cggcaaaatg ctgcacgtcg cccatatcac ctttcagcac ggcgatttcc
     7501 agacagtcgt cgtgattgat atgcacatgc agcgtggcga cggagagatc gtggtgatgg
     7561 tgctgggtgg agacaatgcg gctggctaag tcgcgttttt cgtgttcata cacataggag
     7621 agcaccgcga aaccttgcgt gccgtgctgc tgagtggcct cctgggccag agcgctacgc
     7681 agaatatcgc ggatagcttc ggaacgattg ttataaccac gacgctggct caggctgtcc
     7741 agcgtctcca gtaaatcgtc atcaagcgtg atggtgactc gttgcatttg cgttaaacct
     7801 tttctgtggt gcgacggcgc acggggaatg cgggtaatac cgcgttttgt agcacacgtc
     7861 cggcgtcaga ggaaaaggtt aatttatctc ccaccacctg ggtttcgacg atttgtccgt
     7921 tgtccatcac catcacccgc tgacagaagc gttccaccag gcgtaagtcg tgggtgatga
     7981 acaggcaggc ggtgccaaac tgttgttgta gctttttcag caggcgaatg acgcccgcct
     8041 gtaacacgag atcaaggtta gaaactgctt catccagaat cagcagcttc ggttcgaccg
     8101 ccagcgcgcg agccaggcag acgcgctgga gctggccgcc gcttaactgc ggtgggcgct
     8161 tgtcgagcac gctgtcgtcg agatcgaccg ccttcagcat ttcgctggcg cgcgccagtt
     8221 gttcggattt tttcagtgat agcaggtggc gcatcggttc acgcaggatc tcgcgcacgg
     8281 ttttgcgcgg attcacggcg ctgatggagt cctgaaatac catctgaata tcgcggcgga
     8341 acgctttacg ctgggcgcga ttgagtttcg ccagcggttc gccgcgccag ctaatattcc
     8401 cttgtgaggg agactctaag cctaccagca gccgcgcgag ggtgcttttt ccgcagccgc
     8461 tgcgtcccag cagggcgacg gtttcgccgc ttttcagggt cagggaaacg ttattcagca
     8521 ccgcctgatg ttgatgtttt ccgctaaatc caccgtgggc atagtgatgg gaaaggccgc
     8581 agacgttaag taaggtcatg atgccagctc cataccgtag agggcgagat gagcggaaac
     8641 caggctgcgc gtcaccgtat gtttgggggc gttaaacagc gtttctacat cgccctgttc
     8701 gacaatttta ccttgtgaca tcaccgccac gtcatccgcc agacgcgcca caacgcccat
     8761 atcatgggtg accagcagca ttcccggcgc ttgtttttgc ataatgcttt ccagcagatc
     8821 gaggatgcgc gcctgtgcta ccacgtcgag gtcggtggtc ggttcatcgg cgatgataaa
     8881 cggtgattca cacagcaccg ccatcgcaat catcatgcgc tgcaacatgc cgccgctcat
     8941 ctcgaacggg tacagcttca gcacgcgcgc ggcgttttcc agccccaccg cttctatggc
     9001 agcggtaagc gtagcgtcat cggcgggttt ccctaacgcc aggcaggttt cccgcgcgtg
     9061 ggtgtgcatg gtgtgcagtg gattaaaggc gctgcgcggg ttctgcatga tggtggcaat
     9121 tttgatgccg cgcagggcgc aaggcgaaac cggtttgcca tcggctaaaa tttcccccgc
     9181 cgtctggcga acgccagcgg gcaaaatgcc cagcgtcgcg gcgcaggtta atgatttccc
     9241 gctgccgcta ccgccgacta acgccagcac gcgcccgcgt tgcagggtta acgatacacc
     9301 gtgtaccagc ggctgcgcgg cctgtagcgc gatattacgt agttcaatct gttgcggcat
     9361 tagtgtgcgt gctccgtcac cagatgagga tccagatgat cgcgcagtgc gtcacccacc
     9421 aggttaaagg ccatcacgct gataaacagc gccagccccg gccagaacat ttgcagcggc
     9481 tgggtccaga tatactggcg cgcgtcgtta atcatcacgc cccattcggc ggtcggcgcg
     9541 gtcacaccga ggccaaggaa agacatcccc gcgacgtgca gcatcatatg gccgatatcc
     9601 agcgttgcca gcaccagcag cgaagggatc accgcgcctg ccagatgatc gacaaacacc
     9661 cgcacatggc ccgcaccgga aagccgtgac gccagcacaa actcgcgttg gcgtagtgaa
     9721 atcaccaggc tgcgcaccat gcgtgcatac cacgcccagt gcgacagggc gatggcgata
     9781 attacgttgg tcagcccggt gccgagcacg ccaaccataa agaacgacag aatcgaggtc
     9841 gggaaggtca taaacatatc ggcgacgcgc atggtggcct gatcaacgcg tccgccaatc
     9901 agtccggcgc tgccgccaat caccagccct aatgtcagca ccagcagcag gcaggccatt
     9961 accgaaccga gcgacacgcg ggtcgccgcc atcagccgcg agaaaatatc acgccctaag
    10021 tgatcggtgc ccagccagtg ctgcgcatcc ggcgaaagca ggcgcgacgg cagatcaata
    10081 gcctgagggt catacggcag ccaccactgg ctggtaagcg caatcagcgc cagcagggcg
    10141 ataatgatca gcgccaggcg taccgaccag cgggaagaga ggaaaaagtt cacgcgtgcg
    10201 ctccttcatg acgacgaatg cgcgggtcca gcgcggcgtt gagcaaatcg acaatcaaat
    10261 tacagaccac aaaaaccacc accatcatca gcgtaaagca ctggatcacc ggatagtcac
    10321 ggttaaaaat cgccgacacc gcatagcgcc cgacgcccgg ccaggcaaag atgttttcga
    10381 taatcatcgt cccgccgatc agttcgccga tgtgcatccc cacggcggtg atcatcggca
    10441 gcgaggcatt gcgcaggatg tggcgacgtt cggtctgttt gtcgttcagg ccgcgcagac
    10501 gcgcccaggt gacgtgacgc tgaccggcga cgtccagcat actggcgcgc agtaaacgcg
    10561 cgttaatcgc cagcgacata aaggcaatgg aaaccgcagg caaaatgatg tgctgccagc
    10621 cgccgtaacc catcgcgggt agccattgca gatacaccga aaacgccatc accagtaaaa
    10681 acgccagcca gaagttaggc atcgacacgc caagaaacgc gataaagcgc acggcgaaat
    10741 ccggcagacg gtcgcggtgg cgcgccgccc agataccgag cggtacggaa gtgagcagaa
    10801 ttaataccag cgccgcacct gcaagttcca gcgtggcggg caggaagttc agcatatcgt
    10861 ccagtaccgg gcgttggctg gcgaatgaga taccaaagtc aagatgcagc gccttccaca
    10921 accaggtgcc gtactggacg tacagcggct gatccagtcc cagcatggtg cgggtagagg
    10981 ccagcatctc cggcgtcggc ggcaggttag acagacgcaa ataatcgagc gccgggtcgc
    11041 cggtaccgag gcgcagcatc agaaaaatga tcaccgaggc ggcgagcacc atcgggatca
    11101 gcagcagaaa gcggcgtaat acgtaacgca acattaaggt ttcaccggtt taatctgttc
    11161 gaacggaatt tcggtggcga tcggcgcgta ggggatatta cccagctccg gttttgatac
    11221 caccatcatc gagatgtagc tgataggcag ataaaccgcc tcgtcatgca gacgggtcag
    11281 aatgtcgcga tacagcgcct gacgttgcgt ttcgtcatgg gtcgccagca cttcgccgat
    11341 ctctttatca atcagcggct tatcggctaa tccttgctgt gcctggaagt cagcgtgcga
    11401 cggtacgcgc attgaactca ggaaggcgtg tggatcgtat ggcgcgcccc aggtgcggtg
    11461 gaaaatcatg ccaaaacgac cgtcgcgctg acgagcatag atactgctct cttcttcgcc
    11521 aatcagcgag acatctgcgc caatctggcg catatcagcc tgaatgattt ccgccatcga
    11581 tttgcttaac gcatcggtgc cgatgaacga cagttcaatg cgcagcggct gaccattttt
    11641 ctcgcggatg tctttgcccg caggcagcgt ccaaccggct ttttccagca actctttcgc
    11701 tttctgcgga tcgtactggc gcggtttcag gccgagatta gcgtaaggca cagaaggggc
    11761 aaacagggtg tcggcgacct gctgggtgcc atataacgcg ttatcaatca gcgatttttt
    11821 gtttaccgcg taattaagag cttcacgtac tgccagctcg ttggtggggg ctttggcggt
    11881 attgagcgcc agcatcacgg tttcgatcgg ctgtgacagt tgggtgtggt aagccggatt
    11941 ctggctaaag cgggcgaagg tatcgagcgg taataaccct tcgtttccgt acagcaggtc
    12001 gatatcgcca gtttcaaacg ccaccgcgcg ggtagtcggg tccgggatga cgttaaaggt
    12061 gatcttttta atcgctggct tttcgcccca gtagttttcg ttacggacga agacatcgta
    12121 ctgattcagt ttcgattcct gcaaaatcca tggtccggtg ccaatcggcg ctttaattcc
    12181 gttcatggtt tcatggtttt taaactgcga gggagcgata aaacggaaag gacggggcag
    12241 ggccagttct tgcaggaaag gatagtaggc gcttttcagg gtaatttgca gctctgtttt
    12301 actgagtgct ttaacatcaa caatctggtt tgccagctcc agccaggcgt gacgttgacg
    12361 gttatcgagc actgcgcgga agttttctgc cgccgcctcg gcatcgaacg gttcaccgtt
    12421 ggagaatttc acgtcatcac gcagggtgaa ggtccaggtt ttaccatctt ctgaatgagt
    12481 ccagcttttt gccagccacg ggatcaccga accgtctgcc tgatatttca ccaatggttc
    12541 ataaaccatg ctctgggcga acatctggtt aggcgtgtaa aggtgcgggt ttagtggccc
    12601 gacattcacc ggccaggcgg tggtgatttc atctggtgca gcggcatgga cgataaaaga
    12661 cgcacaagcc agcagcgcaa atagagtgcg gcggagtgtg gagagcatgg taaccccaat
    12721 ggattaaaat agatggcgga aataagtatg acgattttaa gtattcgtca tactgattac
    12781 ctgttctgga tcaatagatg ggcagtcggg tgggcggatc agttaactga atcgatccat
    12841 tgcacactgt cggcggtgag cgtaaaggga gtaggggtgc agatcgccag gcttaaattt
    12901 tcaagctgac aatggctgac tgacagcgag gagtgatagg tgctgtctac gctgacgatt
    12961 tgccaggcgc tgccgccacg ctgtttaacg atggcttctt tgcgcgtcca gatgcgccag
    13021 aacatttcca gttgctgatc aggatgcacg gcgtccatct cagcgtgttc cccgaggctg
    13081 aatacggcgt tcgccagcca gcgccagttg gcgcgcgggc gaatcacttc gatatcgcag
    13141 ccgacttcgc cttcatcact caacagcagg gcgatatcgt caccgctatg gcttaagttg
    13201 aaccatagcg gcgtttccgg cgcaaatgca ggtttgcctt gttcgccata gatgatctcc
    13261 ggtagcgggg aaagcgtgtg cgaaagcaat gcacgccccg ccagccagcg ttcgcgtcgt
    13321 ggaccttgcg gtgcttgctc gcgtaaaccc ggtggcagtg gagctgcgct taaggtcgaa
    13381 actttcccca gaactatccg atacatatca gggccaacgt ttaatggaaa atgaaagtgc
    13441 gtatcgtatc acttgtcgcc tcatcccggt aaccgacttt tcggtctgcc tggccccagt
    13501 aaaatcgcca gtttgctacc gcctttggtg gtttccatcc agattttaca cacactggtt
    13561 aatggcaccg aaagtagcat ccctaccggg ccgagcagcc atccccaaat taacaatgaa
    13621 agaaatacca ccatggtgga catccccagg cgatggccca tcatccgtgg ctctaaaata
    13681 ttgccgatga ccatatggac aactaaaaac aatgcgccga ccagaataca ttcgtaaata
    13741 ccattaaaca gcagcacctg aatcattggc ggtacggcgg aaattaccgc gccgatattg
    13801 ggcacgtagt taagcaaaaa cgccagtact gcccacatca gcgcaaactg tacccccatc
    13861 agctccagcc ccagccagac gatgacacct gtccataaac tgagtagcgt cttcaatgca
    13921 agatagtgcg aaacgccttt aagtgcgcgg tgtaatcccg cgatgtgaat ctgtggatta
    13981 ttcagcgcaa aacgcatttt gtaagggacg tggcgcactt caaacagcat aaaaactacg
    14041 gtcatcacca gcaaaagcac gctcgccatt gccccggaaa gcccggtcat tagcgctgtg
    14101 gtgaaggtaa ccactttttc cgagtccatc cgctgcagca ttcgctccgg cgacatatgc
    14161 aaattaagaa aaggcaacat ctcctgcaat ttaaaaagtt tgcgcgtcag ctccttatta
    14221 aacttcggca gcatagagat aaattcgtta aacgatgccg ccagtacgcc gaccagcgca
    14281 gttagtgcga tcagcatcac caccaccaca atcgtaatgg caatggggcg ttgtactccc
    14341 cgacgaataa accaggtgac gagcgggttg aggacgatgg caaaaaacag cgccagtaac
    14401 agctgcacaa tgatatctgc cgctgcgtga atgcccgcga ggatcactac cagcgaggcc
    14461 agcttgagca gaatgtgcat gcccgtttta tcgggttgag gggtttccat tggggcttcc
    14521 ttgtgacttt ttgtattaag tgtagcggga gtcacgccag caatattctg attcttactg
    14581 ctgattttca cgtcagcacg tggtaaaaat gaaacactgt tgtaaaaatg tggtgatcct
    14641 catgcccgaa cccgtagccg aacccgcgct aaacggattg cgtctgaatt tgcgcattgt
    14701 ctccattgtc atgtttaact tcgccagcta cctcaccatc gggttgccgc tcgctgtatt
    14761 accgggctat gtccatgatg tgatgggctt tagcgctttc tgggcaggat tggttatcag
    14821 cctgcaatat ttcgccacct tgctgagccg tcctcatgcc ggacgttacg ccgatttgct
    14881 gggccccaaa aagattgtcg tcttcggttt atgcggctgc tttttgagcg gtctgggata
    14941 tctgacggca ggattaaccg ccagtctgcc cgtcatcagc ctgttattac tttgcctggg
    15001 acgcgtgatc cttgggattg ggcaaagttt tgccggaacg ggatcgaccc tgtggggcgt
    15061 tggcgtggtt ggctcgctgc atatcgggcg agtgatttcg tggaacggca ttgtcactta
    15121 cggggcgatg gcgatgggtg cgccgttagg cgtcgtgttt tatcactggg gcggcttgca
    15181 ggcgttagcg ttaatcatta tgggcgtggc gctggtggcc attttgttgg cgatcccgcg
    15241 tccgacggta aaagccagta aaggcaaacc gctgccgttt cgcgcggtgc ttgggcgcgt
    15301 ctggctgtac ggtatggcac tggcactggc ttccgccgga tttggcgtca tcgccacctt
    15361 tatcacgctg ttttatgacg ctaaaggttg ggacggtgcg gctttcgcgc tgacgctgtt
    15421 tagctgtgcg tttgtcggta cgcgtttgtt attccctaac ggcattaacc gtatcggcgg
    15481 cttaaacgta gcgatgattt gctttagcgt tgagataatc ggcctgctac tggttggcgt
    15541 ggcgactatg ccgtggatgg cgaaaatcgg cgtcttactg gcgggggcag ggttttcgct
    15601 ggtgttcccg gcattgggtg tagtggcggt aaaagcggtt ccgcagcaaa atcagggggc
    15661 ggcgctggca acttacaccg tatttatgga tttatcgctt ggcgtgactg gaccactggc
    15721 tgggctggtg atgagctggg cgggcgtacc ggtgatttat ctggcggcgg cgggactggt
    15781 cgcaatcgcg ttattactga cgtggcgatt aaaaaaacgg cctccggaac acgtccctga
    15841 ggccgcctca tcatcttaaa acttactgaa taaccagcgt attaatgatg ttttctgcgg
    15901 tggtctgcgc tttttgctga tcgtcagcgg gcagcgtaat ttgcatggtc agcagttgat
    15961 tacccacgtt acccagaata acggaagagt acgccgtctg gcctttcgcg gagataatac
    16021 tgtctaactg ctgcattttg tgacctttca gctcaatggc tttattggtt accacttgca
    16081 gctgcggatc gcggctacgt tgctgatctt ccagacgctt cgccagcacc gccagatctt
    16141 ctttcggatc atcgcccatg atgacgatga ctgctttctg cccggtggcg tcggaccaga
    16201 catgcatgtt attggcctgc gttcccagct taccgctctg gtcggtcata tccgctggca
    16261 gcgagaaact taacttgcca tcaagcaggt tgacgggatt cccggtagcg ttactttccg
    16321 cgaccgaacc ctgcgccgta gcgttagtgt ctttatcatc acaggccgca agccccataa
    16381 ccagcaggcc aattccgaca tatttaacca gattgcgcat tgacttcttc ctttcgataa
    16441 acggccataa cggctcattc atccatctta tcacaactct gataacgaac ctttaactcg
    16501 cctgcaaagc gttgatttcg gatttatctg ccagtctttt caacagcata ttgagtaata
    16561 cgccatacat tggcaggaag aaaacgatac tgattaacac tttgaaacag taatcgacca
    16621 gcgcgatttc catccagtgt tcagccataa aggcatccgg gctacgccag aaggcaatga
    16681 agaaaaaggc cagcgtgtca ctgacgttac cgaacagtgt ggacgctgtc ggtgccagcc
    16741 accagcggcg actttgacgc aggcggttaa aaacgtgcac gtcgaggatt tgccccagcg
    16801 cgtaggccat gaaactggcg gtggcgatac gggcgacaaa caggttgaag tgggcgagtg
    16861 cgccgaatcc ctgccatgaa cccatataga acagcgacga gatgacatag gagattaata
    16921 acgcagggat cattaccgcg aagataatgc gtcgggccag cggtgcgcca aaaatacgca
    16981 cggtcaggtc ggtcgcgaga aagataaacg gaaaactaaa cgcgccccag gtggtatgaa
    17041 aaccaaaaat ggagaccgga agctggacca gatagttact ggaggtgatc accagcagat
    17101 gaaataacga taaccagaac aacgccttat agcgttgagt ttgcgagaaa acgttcatat
    17161 tgtacctttt tgattaacca ttggggtgag ggaacccaat acgtacgaca cgtctgttat
    17221 cttaacataa taacgtgctt accctttttt cgagccgccg catgatactg ctttacgttg
    17281 acaatgcaat ggttaatttt cacgcaatcg ttaacctggt ttgcttacgg actcacaggg
    17341 cgtaaactag cgccgttttt ttaagtgatg agaagaaaat gaccgatctc ttttccagcc
    17401 ctgaccacac actcgacgcg cttggcctgc gctgcccgga accggtgatg atggtgcgca
    17461 aaaccgtgcg caatatgcag cctggcgaaa ctttactgat tatcgccgac gatccggcga
    17521 cgacccgcga tattcctggg ttttgtacct ttatggaaca cgaactggtt gcgaaagaga
    17581 cggatggact gccttatcgt tatttgattc gtaaaggcgg ttgatgggga aagattggct
    17641 tcgatgccgc cttttcccct caccctaacc ctctccccag aggagcgagg ggaccgatcg
    17701 cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa caatcttaac gcattcgctg
    17761 ttaccagcac ggtcgcaccg gtatctgcca gcaccgccag ccatagtccg gtcatcccta
    17821 gcagcgtggt gacgaggaag atccctttca gccccagcgc aatagtgatg ttctggcgga
    17881 tattggcgtg agtggcgcgt gccagttcaa tcatttgcac caggccgcgc aggtggttat
    17941 gggttaatgc tgcgtcggcg gtttccagcg ccacgtctgt gccactgccc attgcaatcc
    18001 caatggcggc agctttcatc gctggcgcgt cgttaatacc atcaccgacc atcgccaggg
    18061 gcgcatgttg attcagcttg gtcaccgctt tgactttatc ttccggcaat aggcccgctt
    18121 taaactccag ccccagctcc ccggcaattg ccgccgctgc gcgtggatta tcgccggtga
    18181 ggatcactcc tttaaccccc agcgcgttca gttcactgat ggcagttgct gcgtcggcgc
    18241 gcagggtatc ctgcaatgca atgataccca gcacgtcatc attacgtact accagcacaa
    18301 ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc aaatgcatca gcgggatgtt
    18361 tcccggcagc gcaaatcaat acgcgctcac cgttaacctg cgcttcaatg ccagacccga
    18421 ccagcgcccg ctgtgattcg gcggtgggaa tggcgagttc agcaacctgt gcttcgcgta
    18481 cgatggcctg cgccagtgga tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca
    18541 gttcagattc actaataccc gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg
    18601 tcagcgtacc ggttttatca aacgccacct gagtaacacg acccagctgt tccagcgccg
    18661 ctccgccttt aatcaacgcc ccacgacgcg ctgccgccgc cagcccggag gtaatcgccg
    18721 caggcgtgga gataactaac gcacacgggc agccaatcag cagcagcgtc agccctttat
    18781 aaatccactc ctgccagctg gcggcaaaca gcagcggtgg caccagcgtc accagcaggg
    18841 cgacggccat aatcgctggc gtataaatac ggctgaaacg gtcgataaac cgctcaatgg
    18901 gggcgcggcg ctcttcggct tcttcgatca gtttcagaat ccggtcaatg gcgctggcac
    18961 ccggttctga cagcacttcc agcgttacca gacggtctac gctggtggct cctgcaggaa
    19021 ctttatcgcc cgtcgcgcgc tccaccggaa tggattctcc ggtcagggcg ctttcatcaa
    19081 aactggcaaa cggtgagagc agtttaccgt cggcaggcaa acgcccacct gcggcgactt
    19141 caatcacatc gccaggccgc aggctgttaa tcgccacctc ttcccgctca ccgttacgca
    19201 ggcgcgtggc ggtttctggt ttcagcgcca ttaacgcgct aacgccctga cgcgcgcggt
    19261 tggcggccca gccttccagt cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt
    19321 cagccgttgc gccaataaac agtgcaccaa tagcggctac gctcattaag gtttcaatgg
    19381 cgaagtagct gccggatttg atcagccgta atgcctgacg agcaatcggg tacagcccaa
    19441 ccagcgtggt cgcgataaac gccagttgcc caaacggatg attaaattgc tccagacccc
    19501 agctgattgc catcatcacg attagcgtaa tcagcggcag attctctttc aggcgcgatg
    19561 cttgtggctc gtcggcggcc tgctcatcgc gcagggaata gcccgctttt tgcaccgcag
    19621 attcaacctg ggcacggatg tcattgtcgg catcgaccac cagtttttcg gtggcgaaca
    19681 gtacctgcac ctgattcaca cctgcaagct ggcgcacggc attttctacc ttgcgcgcac
    19741 aggcggcgca gtccatgccg ctgactttcc agctatagcg ggtgccggag acgttttcag
    19801 agagcgttgg cgagctggaa catgcgccgt cgcagcaaca gtcgttggcg ttctgtaccg
    19861 tggttagcgg tttgaacgca gcaaattgag gggctttctt gccgtgattg tcaggagtcg
    19921 acatggcatc ctccggttaa gttttttctc attaaccgaa ggatacactc tggagtcgac
    19981 tccagagtca agttttatca gagatacagc gagcggacga tgaggaaatg accggcaaag
    20041 tagcaggccg cagcaatcgc gttatctgcc cggaagcggc ggcgatagtg gctacccagc
    20101 cagacaaagt tactgataaa cagcaatgaa gcgccaacaa acgcagagag tgccggagcg
    20161 gtcggacgga agaaccacag ttcacctgcc agccacacca tcaccagcgt catgccgata
    20221 aacgtgcaga taggccaacg atactcttcc agccgcgtcc agataatcgc cagtaacagc
    20281 gcgcccagaa ccagcaacac cagtggcaga ggccagaaga aagagagcgt catctgactg
    20341 gcgaaataga tggtgtacag caggtgcgag aggaaaaacg cgccgatggc gtacatcaga
    20401 cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg aggcgcacag ccctgccagc
    20461 accagatagc taatggcgtc gaacatcggc gcttgccagg ccagtaacag caggagaaga
    20521 agggttaacg gtttaaacac ccagcgttgc caggttggcc cacgatacga tgcatccaca
    20581 gatagccatg cggaaagaca gacagcgata aacgaccaaa gcatcttagt tccttgaatt
    20641 cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg
    20701 acggatgaca acaccataac tggcagggta tgcttatttc cgcattttcc gatgagggat
    20761 aaagatgagc aaaccacctc ttttctttat tgttatcatt ggcttaattg tcgtcgccgc
    20821 atcgtttcgt tttatgcaac agcgacggga aaaagctgat aatgatatgg ctccgctcca
    20881 gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg
    20941 ccagcaggaa gtgactccgg caggcaccag tatgcgctat gaggcaagct tcaaaccgca
    21001 aagcggagga atggagcaga cgtttcgcct cgacgcccag cagtaccacg ccctgacagt
    21061 gggcgataaa ggtacgctga gctataaagg aacgcgcttt gtcagctttg taggcgaaca
    21121 ataaggttat tttttaactt taaatttctt ctgccagacc agcagttcaa acacgccgaa
    21181 aaggaaaatc cgcacctttt cggcggtggt catctgcggg ccatctttcg gtaaagtgga
    21241 tttcaatagc gccagttgca taccatgcat cagcacggta aaaatcagcg ccacgttaac
    21301 gaaaatattc agcgggcgcg ggaagggatg caccagattg aggattaaaa atccccaaac
    21361 gcagagcatt aacaaacgac caatattaat cagcatcact ttctccttgt gcttcgcgtt
    21421 gatacagccg ataagccacc tgacccgcca ctttttcccg atgtaatgac cagtttgccg
    21481 gaacagtggg cagaccgttt tcgacttcgc tttcgacata aatcagggct tcgtcagcca
    21541 gccagccgtt atcttccagt aaatttatcg tctcttctaa caagccacgg cggaacggtg
    21601 gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc caggaatgac attgcgttgc
    21661 tgttcaccac gcgtgcattg cctgctttta gtgtcgccag attcttaatt aactgctgag
    21721 aaactgcgcg atccatctca atcaacgtgg cccccgcagc gtagcgcgat aacgcttcca
    21781 gccccagcgc gccgctcccg gcgaagcaat ccagacattg ggcgtcaaca atgaccggag
    21841 ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat
    21901 caggaaccgg gagtttacgg cctcgccact gcccgccaat aatgcggatt tggccgctgc
    21961 cggaatgatt cggttttttc atgataaatt gctcaatccg ccagataaca catacttgca
    22021 ggcggtgatg tgaattaagt taagtgatag actatttcat cattttttta gctgctatgt
    22081 acatagcgtt aacgctgtgc catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa
    22141 gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc aaaaagagca gaccccggaa
    22201 aaagagacag aagttcagaa tgaacaaccg gttgtagaag aaatcgttca ggcgcaagag
    22261 cctgcgaagg cttctgaaca agccgtcgaa gagcaaccgc aggcgcatac tgaagccgag
    22321 gcggaaactt ttgctgccga cgttgtggaa gtcactgaac aggttgctga aagtgaaaaa
    22381 gcgcagcctg aagcggaagt cgttgcacag ccggaaccgg tcgtagaaga aacggcggag
    22441 ccagtggcta tcgaacgtga agagctgccg ttgccggaag acgtcaacgc cgaagaggtt
    22501 tcgccagaag agtggcaggc tgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa
    22561 gaagcggcta aagaagaaat caccgacgaa gagctggaag cacaggcgct ggctgccgaa
    22621 gcggcagaag aggcggtgat ggtggttcct ccggtagaag agcagccggt ggaagaaatc
    22681 gctcaggagc aggaaaaacc gaccaaagaa ggtttcttcg cgcgcctgaa acgcagcctg
    22741 ttaaaaacca aagaaaatct cggttccgga tttatcagcc tgttccgcgg taaaaaaatc
    22801 gacgatgatc tgtttgagga gctggaagaa cagctgttga tcgccgatgt gggcgtggaa
    22861 accacacgta aaattatcac caatctgacg gaaggcgcat cccgcaagca gcttcgtgat
    22921 gccgaggcgc tctatggcct gctgaaagaa gagatgggcg agattctggc gaaagtcgat
    22981 gagccgctga atgttgaagg taaaacgccg ttcgtgatcc tgatggtggg cgtcaacggt
    23041 gtgggtaaaa ccacgacgat tggtaagctg gcgcgtcagt ttgagcagca gggtaaatcg
    23101 gtgatgctgg cggcgggcga tactttccgt gcagctgcgg ttgaacagct tcaggtctgg
    23161 ggtcagcgta acaatatccc ggtgattgcc cagcatactg gtgcggattc cgcttcggtt
    23221 atcttcgacg ccattcaggc tgcgaaggcg cgtaatgttg acgtactgat tgccgatacc
    23281 gccggacgcc tgcagaacaa atcgcacctg atggaagagt tgaagaaaat cgtccgcgtg
    23341 atgaagaaac tcgacgttga agcgccgcat gaagttatgc tgactatcga tgccagcacc
    23401 gggcagaacg cggtaagcca ggccaaactg ttccatgaag ctgttggctt aaccggcatc
    23461 acgctaacga aactggacgg cacggcgaaa ggcggggtaa ttttctcggt ggctgaccag
    23521 tttggtatcc ctatccgcta cattggtgtc ggcgaacgta ttgaggattt gcgtccgttt
    23581 aaggcggacg actttataga ggcacttttt gcccgagagg attaacaatg attcgctttg
    23641 aacatgtcag caaggcttat ctcggtggga gacaggcgct gcagggcgtt acgttccata
    23701 tgcagccggg tgagatggcg tttctgaccg gtcattccgg cgcagggaaa agtaccctcc
    23761 tgaagctgat ctgtgggatt gagcggccca gcgccgggaa aatctggttt agcggccatg
    23821 acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg ccgccagatt ggcatgattt
    23881 tccaggatca ccatctactg atggaccgta ctgtctacga taacgtggca atcccgctga
    23941 ttatcgccgg tgccagcggt gacgatattc gtcgccgggt gtcggcggca ctggataaag
    24001 tcgggctact ggacaaagcg aagaacttcc ctattcagct ttctggcggt gaacaacagc
    24061 gtgttggcat tgcccgcgcg gtggtgaaca agcccgcggt actgctggcg gacgaaccga
    24121 ctggtaacct ggacgacgcg ttgtcggaag ggattctacg tctgtttgaa gagtttaacc
    24181 gcgttggggt aaccgtattg atggcaacgc acgacatcaa cctgatctcg cggcgttcct
    24241 atcgcatgct caccctgagc gatggtcact tgcatggagg cgtgggccat gaataagcgc
    24301 gatgcaatca atcatattcg gcagtttggc gggcgtctgg atcgcttccg taaatcggtc
    24361 ggcggctctg gcgacggcgg tcgtaacgcg ccaaaacgcg cgaaatcctc gccaaaaccg
    24421 gtaaatcgca aaaccaacgt tttcaacgaa caggtgcgct atgccttcca cggcgcattg
    24481 caggatctga aaagcaaacc gttcgccacg tttttaacgg tgatggttat cgccatctcc
    24541 ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg ttaaccaggc ggcgacgcaa
    24601 tattatccgt caccgcaaat cactgtttac ttgcaaaaaa cgctggacga tgacgccgca
    24661 gcaggcgtgg tagcacagct acaggccgag cagggcgtgg agaaagtgaa ctatctttct
    24721 cgtgaagacg cactgggcga gttccgtaac tggtctggtt ttggtggtgc gctggatatg
    24781 ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc cgaaactcga ttttcagggg
    24841 acggaatcac tgaatacgct gcgtgatcgt atcacccaga ttaacggcat tgacgaagtg
    24901 cggatggatg acagctggtt tgcccgtctg gcggcgttga ccgggctggt cgggcgcgtt
    24961 tccgcgatga tcggcgtgtt gatggtggca gcagtgttcc tcgtcatcgg caacagtgtg
    25021 cgtctgagca tctttgctcg ccgtgactcc attaacgtgc agaaactgat tggtgcgacg
    25081 gatggtttca tcctgcgccc gttcctgtac ggaggcgcat tgctgggatt ttctggcgca
    25141 ttgctgtcat taattttgtc agaaattctg gtactgcgtt tgtcatcggc ggttgcagaa
    25201 gtggcacagg ttttcggaac gaagtttgat atcaatggtt tatcgttcga tgaatgcctg
    25261 ctattgctgc tggtatgctc gatgattggc tgggtggcag cgtggcttgc cacggtacaa
    25321 catttacgcc actttacgcc tgaataataa aagcgtgtta tactctttcc ctgcaatggg
    25381 ttccgtagca gggaaagaga tcccgttgtc tcttcccggt atttcatctc tatgtcacat
    25441 tttgtgcgta atttattcac aagcttgcat tgaacttgtg gataaaatca cggtctgata
    25501 aaacagtgaa tgataacctc gttgctctta agctctggca cagttgttgc taccactgaa
    25561 gcgccagaag atatcgattg agaggatttg aatgactgac aaaatgcaaa gtttagcttt
    25621 agccccagtt ggcaacctgg attcctacat ccgggcagct aacgcgtggc cgatgttgtc
    25681 ggctgacgag gagcgggcgc tggctgaaaa gctgcattac catggcgatc tggaagcagc
    25741 taaaacgctg atcctgtctc acctgcggtt tgttgttcat attgctcgta attatgcggg
    25801 ctatggcctg ccacaggcgg atttgattca ggaaggtaac atcggcctga tgaaagcagt
    25861 gcgccgtttc aacccggaag tgggtgtgcg cctggtctcc ttcgccgttc actggatcaa
    25921 agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc gtcaaagttg cgaccaccaa
    25981 agcgcagcgc aaactgttct tcaacctgcg taaaactaag cagcgtctgg gctggtttaa
    26041 ccaggatgaa gtcgaaatgg tggcccgtga actgggcgta accagcaaag acgttcgtga
    26101 gatggaatca cgtatggcgg cacaggacat gacctttgac ctgtcttccg acgacgattc
    26161 cgacagccag ccgatggcac cggtgctcta tctgcaggat aaatcatcta actttgccga
    26221 cggcatcgaa gatgataact gggaagagca ggcggcaaac cgtctgaccg acgcgatgca
    26281 aggtctggac gaacgcagcc aggacatcat ccgcgcgcgc tggctggacg aagacaacaa
    26341 gtccacgttg caggaactgg ctgaccgtta cggtgtttcc gctgaacgtg tgcgccaact
    26401 ggaaaagaac gcgatgaaaa aacttcgcgc cgctattgaa gcgtaatttc cgctattaag
    26461 cagagaaccc tggatgagag tccggggttt ttgttttttg ggcctctaca ataatcaatt
    26521 ccccctccgg caaaacgtca atccccacgc agattgttaa taaactgtca aaatagctat
    26581 tccaatatca taaaaatggg gtatgtttta gcagagtatg ctgctaaagc acgggtagtc
    26641 atgcataaaa cgaaataaag tgctgaatca caacaccaca acacacgtaa taaccagaag
    26701 aatggggatt ctcaggatga acataaaggg taaagcgtta ctggcaggat gtatcgcgct
    26761 ggcattcagc aatatggctc tggcagaaga tattaaagtc gcggtcgtgg gcgcaatgtc
    26821 cggtccggtt gcgcagtacg gtgaccagga gtttaccggc gcagagcagg cggttgcgga
    26881 tatcaacgct aaaggcggca ttaaaggcaa caaactgcaa atcgtaaaat atgacgatgc
    26941 ctgtgacccg aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg gcattaaata
    27001 cgtgattggt cacctctgtt cttcatcaac gcagcctgcg tctgatatct acgaagacga
    27061 aggcatttta atgatcaccc cagcggcaac cgcgccggag ctgaccgccc gtggctatca
    27121 gctgatcctg cgcaccaccg gcctggactc cgaccaggga ccgacggcgg cgaaatatat
    27181 tcttgagaag gtgaaaccgc agcgtattgc tatcgttcac gacaaacagc aatacggcga
    27241 agggctggcg cgtgcagtgc aggacggcct gaagaaaggc aatgctaacg tggtgttctt
    27301 tgatggtatc accgccgggg aaaaagattt ctcaacgctg gtggcgcgtc tgaaaaaaga
    27361 gaatatcgac ttcgtttact acggcggtta tcacccggaa atggggcaaa tcctgcgtca
    27421 ggcacgcgcg gcagggctga aaactcagtt tatggggccg gaaggtgtgg ctaacgtttc
    27481 gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc cgaagaacta
    27541 cgatcaggtt ccggcgaaca aacccattgt tgatgcgatc aaagcgaaaa aacaggaccc
    27601 aagcggcgca ttcgtttgga ccacctacgc cgcgctgcaa tctttgcagg cgggcctgaa
    27661 tcagtctgac gatccggctg aaatcgccaa atacctgaaa gcgaactccg tggataccgt
    27721 aatgggaccg ctgacctggg atgagaaagg cgatctgaaa ggctttgagt tcggtgtgtt
    27781 tgactggcat gccaacggca cggcgaccga tgcgaagtaa tcattaatcg gcaactttgg
    27841 gttgccgcca aattgctaat atcgagtacg ttgcttcatg ccggatgcgg cgtaaacgcc
    27901 ttatccggcc tacaagatcc aaagaaatca gtaaattgca acacacattg taggcctgat
    27961 aagcgtagcg catcaggcaa tttctcgttg gtcatcactt ttgaaatcaa cttgacgatt
    28021 aacgcttctc ccagccgccc tgctgtgccg taaaccccag tgcctgcata aacgccgtca
    28081 tcacaccgcg atcttccacg ccagcatccg ccatccacca gcatgaaacg ccaggattgg
    28141 tacgcaaaac ctcttccagc agatattgcc ccacaccgcg acgtcgggtg acttcccgca
    28201 cgcgtagaga atccagtgcc ccctcggtgc cgcttacggt tacccgcacg gcagcgagca
    28261 ggcgctcgtt aaaacgcgcg gcgtagatac ggtggttatc gtcaacctgt aacgaggaag
    28321 gggaatactc cggccagatc ttttgcaggt caatccggtc ctggtcgcta aagttttcta
    28381 atcgaatgat ggtcagtttc atggggaacc cgtgtaaatc acaaaagtgg aaccagtgta
    28441 gcgaaataat ttaatcggag gctttctctt ttttatttct tttggcaggt gattaatttt
    28501 ttaacagcaa taattacaaa gttaaaacat tagagaatga aaaataccca gcataatccc
    28561 ctgaatggta gt
//
LOCUS       DAZFFI010000054        28259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFFI010000054 DAZFFI010000000
VERSION     DAZFFI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.054"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHJ12_002742"
     CDS             complement(<1..209)
                     /locus_tag="ACHJ12_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4990507.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJ12_002743"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJ12_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4990508.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJ12_002744"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJ12_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4990509.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJ12_002745"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJ12_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4990510.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJ12_002746"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJ12_002746"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4990511.1"
                     /translation="MLHTLHRSPWLTDFAALRRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJ12_002747"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJ12_002747"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4990512.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJ12_002748"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJ12_002748"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4990513.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJ12_002749"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJ12_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4990514.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJ12_002750"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJ12_002750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4990515.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJ12_002751"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJ12_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4990516.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJ12_002752"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJ12_002752"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4990517.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJ12_002753"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJ12_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4990518.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHJ12_002754"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHJ12_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4990519.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJ12_002755"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJ12_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4990520.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJ12_002756"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJ12_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4990521.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJ12_002757"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJ12_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4990522.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJ12_002758"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJ12_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4990523.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJ12_002759"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJ12_002759"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4990524.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJ12_002760"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJ12_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4990525.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHJ12_002761"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHJ12_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4990526.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJ12_002762"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJ12_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4990527.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJ12_002763"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJ12_002763"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4990528.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJ12_002764"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJ12_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4990529.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJ12_002765"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJ12_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4990530.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJ12_002766"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJ12_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4990531.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJ12_002767"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJ12_002767"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4990532.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJ12_002768"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJ12_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4990533.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJ12_002769"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJ12_002769"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4990534.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJ12_002770"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJ12_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4990535.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHJ12_002771"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHJ12_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4990536.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHJ12_002772"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHJ12_002772"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4990537.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6830 a         7598 c         7139 g         6692 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cgcggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtatctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcgatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 accacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gccgcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acaccttccc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctagac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagct tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg cagccgtcac gccgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcactgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg acgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcataatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggagaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccacgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggccacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtacc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaacatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact ctcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgcaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 actattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag cacctggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gtgggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggcaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggctattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggta gcgaaatacg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgggtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggccaac aacgatcaga
    27301 aagccattac tgaaaccacc gaacagttaa tcaacgaacc gcttgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgcgtac
    27541 cccaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aactggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27901 gaatgccagg cgaaatgccg gtggcaattg tcgaaaacgg tacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt attgttctt
//
LOCUS       DAZFFI010000055        28258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFFI010000055 DAZFFI010000000
VERSION     DAZFFI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.055"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..794
                     /gene="ybeU"
                     /locus_tag="ACHJ12_002773"
     CDS             87..794
                     /gene="ybeU"
                     /locus_tag="ACHJ12_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4990538.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            791..1522
                     /gene="djlC"
                     /locus_tag="ACHJ12_002774"
                     /pseudo
     CDS             791..1522
                     /gene="djlC"
                     /locus_tag="ACHJ12_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(1800..2384)
                     /locus_tag="ACHJ12_002775"
     CDS             complement(1800..2384)
                     /locus_tag="ACHJ12_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990539.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVHGYGDEPSMKKEPIEQAKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKASA"
     gene            2552..2800
                     /locus_tag="ACHJ12_002776"
     CDS             2552..2800
                     /locus_tag="ACHJ12_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4990540.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            2802..4892
                     /locus_tag="ACHJ12_002777"
     CDS             2802..4892
                     /locus_tag="ACHJ12_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4990541.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCRHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPIKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWGGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKQQEPWLENIYRPVGNAVIVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            4964..5896
                     /locus_tag="ACHJ12_002778"
     CDS             4964..5896
                     /locus_tag="ACHJ12_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4990542.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            5899..6120
                     /locus_tag="ACHJ12_002779"
     CDS             5899..6120
                     /locus_tag="ACHJ12_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990543.1"
                     /translation="MAVFYIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            6133..6387
                     /locus_tag="ACHJ12_002780"
     CDS             6133..6387
                     /locus_tag="ACHJ12_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990544.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            6389..6670
                     /locus_tag="ACHJ12_002781"
     CDS             6389..6670
                     /locus_tag="ACHJ12_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO4990545.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            6667..6939
                     /locus_tag="ACHJ12_002782"
     CDS             6667..6939
                     /locus_tag="ACHJ12_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990546.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            6944..7237
                     /locus_tag="ACHJ12_002783"
     CDS             6944..7237
                     /locus_tag="ACHJ12_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990547.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPVIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            7249..7779
                     /locus_tag="ACHJ12_002784"
     CDS             7249..7779
                     /locus_tag="ACHJ12_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4990548.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            7877..8419
                     /locus_tag="ACHJ12_002785"
     CDS             7877..8419
                     /locus_tag="ACHJ12_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990549.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIET
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREIKG
                     CIPEECQEIKHSEYVALTEE"
     gene            8423..8956
                     /locus_tag="ACHJ12_002786"
     CDS             8423..8956
                     /locus_tag="ACHJ12_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990550.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            8956..9471
                     /locus_tag="ACHJ12_002787"
     CDS             8956..9471
                     /locus_tag="ACHJ12_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990551.1"
                     /translation="MFFKTSNPSALAAWQKYQQDYKKVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALCAASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVEWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            9475..10026
                     /locus_tag="ACHJ12_002788"
     CDS             9475..10026
                     /locus_tag="ACHJ12_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO4990552.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHKRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
     gene            10023..10334
                     /locus_tag="ACHJ12_002789"
     CDS             10023..10334
                     /locus_tag="ACHJ12_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990553.1"
                     /translation="MITTLFVESDEPLVCVAGMSVCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            10349..10699
                     /locus_tag="ACHJ12_002790"
     CDS             10349..10699
                     /locus_tag="ACHJ12_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990554.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTW
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            10715..11047
                     /locus_tag="ACHJ12_002791"
     CDS             10715..11047
                     /locus_tag="ACHJ12_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990555.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            11040..11237
                     /locus_tag="ACHJ12_002792"
     CDS             11040..11237
                     /locus_tag="ACHJ12_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990556.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            11227..11523
                     /locus_tag="ACHJ12_002793"
     CDS             11227..11523
                     /locus_tag="ACHJ12_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990557.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            11520..12029
                     /locus_tag="ACHJ12_002794"
     CDS             11520..12029
                     /locus_tag="ACHJ12_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO4990558.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMLRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            12099..12524
                     /locus_tag="ACHJ12_002795"
     CDS             12099..12524
                     /locus_tag="ACHJ12_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4990559.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            12596..13096
                     /locus_tag="ACHJ12_002796"
     CDS             12596..13096
                     /locus_tag="ACHJ12_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4990560.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            13131..13559
                     /locus_tag="ACHJ12_002797"
     CDS             13131..13559
                     /locus_tag="ACHJ12_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990561.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            13543..13761
                     /locus_tag="ACHJ12_002798"
     CDS             13543..13761
                     /locus_tag="ACHJ12_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990562.1"
                     /translation="MPIPAEWLADCLVPPVPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            13772..13999
                     /locus_tag="ACHJ12_002799"
     CDS             13772..13999
                     /locus_tag="ACHJ12_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4990563.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            13980..14291
                     /locus_tag="ACHJ12_002800"
     CDS             13980..14291
                     /locus_tag="ACHJ12_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO4990564.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTN
                     E"
     gene            14284..14574
                     /locus_tag="ACHJ12_002801"
     CDS             14284..14574
                     /locus_tag="ACHJ12_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO4990565.1"
                     /translation="MSNFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            14577..15158
                     /locus_tag="ACHJ12_002802"
     CDS             14577..15158
                     /locus_tag="ACHJ12_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO4990566.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            15158..16822
                     /locus_tag="ACHJ12_002803"
     CDS             15158..16822
                     /locus_tag="ACHJ12_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990567.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNSRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYCEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            16822..18411
                     /locus_tag="ACHJ12_002804"
     CDS             16822..18411
                     /locus_tag="ACHJ12_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO4990568.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            18395..19715
                     /locus_tag="ACHJ12_002805"
                     /pseudo
     CDS             18395..19715
                     /locus_tag="ACHJ12_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
     gene            complement(19717..19796)
                     /locus_tag="ACHJ12_002806"
     tRNA            complement(19717..19796)
                     /locus_tag="ACHJ12_002806"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            19837..20310
                     /locus_tag="ACHJ12_002807"
     CDS             19837..20310
                     /locus_tag="ACHJ12_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO4990569.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            20487..21611
                     /locus_tag="ACHJ12_002808"
     CDS             20487..21611
                     /locus_tag="ACHJ12_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO4990570.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            21611..22558
                     /locus_tag="ACHJ12_002809"
     CDS             21611..22558
                     /locus_tag="ACHJ12_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO4990571.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            22602..23000
                     /locus_tag="ACHJ12_002810"
     CDS             22602..23000
                     /locus_tag="ACHJ12_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO4990572.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAEAEAEAGQDAPWKKTGNKAKQARA"
     gene            22997..23416
                     /locus_tag="ACHJ12_002811"
     CDS             22997..23416
                     /locus_tag="ACHJ12_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO4990573.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            23413..23973
                     /locus_tag="ACHJ12_002812"
     CDS             23413..23973
                     /locus_tag="ACHJ12_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO4990574.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWNDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            23974..24219
                     /locus_tag="ACHJ12_002813"
     CDS             23974..24219
                     /locus_tag="ACHJ12_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO4990575.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            24216..25715
                     /locus_tag="ACHJ12_002814"
     CDS             24216..25715
                     /locus_tag="ACHJ12_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4990576.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFLRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            25724..26089
                     /locus_tag="ACHJ12_002815"
     CDS             25724..26089
                     /locus_tag="ACHJ12_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4990577.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            26104..26580
                     /locus_tag="ACHJ12_002816"
     CDS             26104..26580
                     /locus_tag="ACHJ12_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO4990578.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            26707..>28258
                     /locus_tag="ACHJ12_002817"
     CDS             26707..>28258
                     /locus_tag="ACHJ12_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4990579.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPS"
BASE COUNT         6826 a         6958 c         8093 g         6381 t
ORIGIN      
        1 attttctttt tattttgaaa gcgtcaagtt tatactcatc aataataaaa agtagtcagt
       61 taatgcactt aagggattat taacaaatga ataaggaaga acaatatctt ctttttgcgc
      121 tttctgcgcc catggaaata cttaatcagg gttgcaaacc cgcgcatgac tcgccaaaaa
      181 tgtataccgg gataaaagaa ttcgatctct catcatcctg gggaataaat aatcgtgatg
      241 atttaattca gactatttat cagatgactg atgatggtca tgccaatgat ttagctggct
      301 tatatctgac atggcatcgc tcttcccctg aagagtggaa agcattaatt gctggtggct
      361 cggagagagg gttgatttat acccaattcg ttgcccaaac cgctatgtgc tgtggagaag
      421 ggggaatcaa agcctgggat tatgttcgaa tggggtttct gtcacgggtc ggtgtgctca
      481 acaactggct gacggaagaa gagagtttat ggctgcaatc gcgcgtctat gtacgagctc
      541 accattacta tcacagctgg atgcactatt tttccgccta ttcgttagga cgtctctact
      601 ggcaatcttc tcagtgcgaa gacaacactt cgctacgcga ggcgctaacc ctgtacaaat
      661 acgatagcgc cgggagtcgg atgttcgaag agctggcagc gggaagcgat cgtttctatg
      721 ccacgcttcc ctggcagcca ttaactgtcc agcctgaatg cccggtaacg cttaaggatg
      781 tgagcgacct atgaaaacat gttggcaaat cctcgaaatt gaaagcacga cgcaaataga
      841 cattatccgc caggcttatc ttgctcgctt accgttatgt catcccgaaa ccgatccgca
      901 ggggtttaaa gcattacgcc aggcctatga agaggcgctg cgactggcgg taaatcctgt
      961 cggggaagca gataatgaag ataaagatgc cgccgctgaa catgaaatac tacgtgcatt
     1021 caggacatta ctggattcag aaagtgatcg ttttcagccc tccgcctggc agaaatttat
     1081 tcagcaatta aatacctgga acatggagga tgtcgatcaa ttacgctggc cgctgtgtgc
     1141 aatcgctata gaagcgcgat atctttcatt aaattgtgct tctttgctgg cagagcgttt
     1201 gaactggcat tcgtttaatg acagcgaagg aatggatgag gaagaaaggg aggcttttct
     1261 tgaggccatt caggctggtg attgtttcga tttccttagc cttctggaat atcccgtcgc
     1321 gttacagaac cagactgttg aatattactt cgcgctggaa cgttgctgcc gttaccatcc
     1381 tgactatgtc actgcgtttt tggcgatgga aggtccgtgg ttcattcctg atgatgcaaa
     1441 gttacatcgc aaactgttgc gctggtacag ctcggtgcaa acaggtatgg cggaactcat
     1501 tcctgtagcc aaacagtggc aagcggatgt aaccaggtga atcatttgat accaacgagg
     1561 gttttagtga aaggaggtga aatcacctgt aagatactga aataaaacag gtgatgtctg
     1621 gatgagcgct taaagtctat ttgataccga ttgtgcaata attaatcatt tattctaaaa
     1681 tcggcaaaac ttttgatact acggattgac gcttaaaatg gctttaaaaa ggcttaaaac
     1741 gtttttctcc agattgtctt taaaggagtg tactacagca caaaaattgc accactccct
     1801 tacgcactag cctttttctg ggttacattt aaatgattaa ccttagcttc acattcttca
     1861 taaatctctc ttacagtatc atcgggtaac tcttgtagac tcaatgctag tgctcttttg
     1921 actccgctca atgagtgcag tttttgaata tctgtatcaa gcaaaacggt tagtagttcg
     1981 gccccttttc tgatcaataa gttggacaaa atctcttgtt cagatgaagt tagcgagtcc
     2041 cacgccctca tgatgatgga atcactatca tcaattcttt gtttttttgt tctttttgct
     2101 tgttcaattg gttctttttt catgcttggc tcatcaccat aaccatggac tagccactcc
     2161 atagatacat gttctctttc tgcaatgttc tggagatggt gctttcttgg ttgtggtggc
     2221 ggctgattgt ttctcctttt gtagtagttc tgcaatgtct gaacgtttat tccccatgct
     2281 ctagccgcct cacttctcga tttgtaacgc tctgcaagac gaaaaatccg ttcgatcact
     2341 ggttcttttc cttcgtcgag aaaagaactg tttttcatgt tcatgctctc aggttccttt
     2401 gtatttatct tattgatatt aataacaaaa acactacaaa gaataaaaag aacaaaatac
     2461 cttgatctgg ttcttttgtt tgttctatta tttacccgta cagatatccg cacgaatatc
     2521 cgcgcgggta actttttaga atagaggaag tatgagtaga agagaagttt cagatcacga
     2581 ttggccgcct gaaatcatca aggcgcgctt acacatggct ggcttatcac tgcgctcttt
     2641 atctttaaag gctggttaca gcagggattc gctgaaaagc gtattacgta ctccttgccg
     2701 accgtatcag caaataattg ctgatgctct tggggtatcg cctgaagaaa tctggcccag
     2761 tcgataccag gttaagagct atatgagaaa ggcgtcatga tatgtatgtc attgcaaaag
     2821 aactgattgg cgcgcccgga atgcctgcta caacaaaagg tattcgccag gcattacaac
     2881 gttacgtaca agggaaaagc tgttgttccc gtcgtcgctc aggctctaaa gcaactgaat
     2941 acagtatcga ctgtttacct gaagtgacgc agcaggcatt acgtgaacgc tacgccctgc
     3001 aactgatgac gcaaaaagcc gatgaatcac cggctccggt ggtgacaaag gccagacgct
     3061 cgcctgccgt ggttgatgcg gtggaggcat atcgcggatc accacaactg atggttgaac
     3121 gccttaatgc cctgactgaa aaccagcgcc aggtggctga tgcacgaatc gcgatcgtca
     3181 gcgaagtgat gaaagtcgcg caacaacccg gtttcagctg tgcgaaagct atccggttta
     3241 tcgttgacaa cctggcacgt tcgcagctgg acgagcgcat tgtggcaatg gttgagacgg
     3301 cgaacgccaa aaagggaaac agccgtgcgt tgagtgaaat cacgctgaaa cgctggattg
     3361 cagcctttaa caaggcgcag aacgccgccg aacgcctgct tttgctggca ccgggtaagc
     3421 gccaggaaat aaaagccgaa gatattaact ggctgcccga atttctggcg cagtatcgcc
     3481 agtcaaacgg ccgaccaatg accgaggcct acgaggattt tgtcgctgaa tggcagcacc
     3541 ggcacgctga tgagccttat atgctcgata tcatgccgtc ttatgacacc attcgccgcg
     3601 caatgaagaa actgccggaa gtggtgaaac aaaaaggccg ggtgaccggc agtgaatatc
     3661 gccagcttga gggattcacg cgccgcgact ggtcaaaaat gccggtgaat tatgtctgga
     3721 ttggtgacgg tcacggcatg aagctgaaat gcaggcaccc ggttcacggg cggccatttg
     3781 caccggaagt gacctttgtt atcgacggtg gcacgcgctt tgtggtgggc tggagccttg
     3841 acctggctga aaatgttttc gccgtagccg gtgccataca gcacggtatt cgtaatcacg
     3901 gcaaaccgtt tctgtattac tcggataacg gttccgggga aaccgccgac atcctggata
     3961 aggaagttgt ggggattctg ccgcgactgg ggattaatca cccgacaggg attgccggta
     4021 acccgcaggg acggggcatt atcgaacggc ttaaccgcac attaccgatg cgcatagccc
     4081 gtaaataccg cacctatttc gggaaaggtg cagatcgcga gacgttacgt aaaaccaacc
     4141 gcgatttacg ctcggcattc agtgccctgc aacagggcaa acggctgaac gcccggcagc
     4201 agtcagcgat gcgtgattta ccgtcctggg ctgaactgat tgatgccatt cgtgatggtg
     4261 ttgagtggta caacaaccgg ccgcacgatg aattacccat aaaaccgaac ggcaagcatt
     4321 acagcccggc ggagttcaga aaaaaacgcc tggcggaaga ggacacggaa attgaatggc
     4381 tgtccgatgt tgagctgcgg gatatgttcc ggccgatggt ggaacgccct gtaagacgct
     4441 gtgaaatacg ctggctgaat aatatttact acgctcccga gctgcgtgat gagcatggcc
     4501 gcaaggtgct tatcagctat gacattcatg atgccgaacg aattaccgta cgtcgcctgg
     4561 atggcagcgt gatttgcgag gcggtatggg gcggcaataa acgcgaagcc ttcccggtca
     4621 gcgcggaata ctacaaacag cagcaacgcc ttaaaggcat gcgtaaacgc gcagaggaaa
     4681 aaatccgtga tgccgaggat gaggtggtca acgtgctgga gcacaagcag caggagccat
     4741 ggctggaaaa catctatcgc cctgtgggta atgcggtgat cgttcagcaa ccggtcgctg
     4801 acgatgaacc tgatgaagaa tacgagcgta atttccagcg gggattgcag ttgctggaag
     4861 cgaaattaaa agaaaatgac ccgctggcct gaaataaaaa aataacccga gcggcgactc
     4921 aggttatttg attaaacaag gtatcaaatg agaggttaat aatatgactg atattaacga
     4981 tgtaatcaag accattgatg aacttattga tggcggcgta ctgacgcagt atgccatcgc
     5041 cagagaggcg ggaatttccg acggcacatt atcggctttc cgtaagggaa aatataaagg
     5101 cgataacgct gctgtggctg cttccctgcg ttcctggtat gagaactgga ataaacaaag
     5161 cgcactgccg gaaccgccgc agtttgtgga aacgcagaca gtccaggagc tgcgcgcact
     5221 gtttcaggcg gttcgtctga tgggctgtat taacgttatt gtgggcgtac cgggtgtggg
     5281 taaaacggcc actgcccgta attactgcca ggagcaacca aacacctgga tgatcaccct
     5341 gtcacccgcg cactccagcg tcacggagtg tctgctggag ctggccgatg cgctggggat
     5401 tgattacacc cgcgcgaaca aaggggcatt atcccgcgcc atccgccgtc gcctgatggg
     5461 aacgcgtgga ctggtgattg tggatgaggc ggatcatctt ggtattgacg gtctggagca
     5521 actccgggca attcaggacg ccacggggat cgggatggtg cttattggta acccgcgcgg
     5581 attgtttaaa ggtggacgcc gcgcctttga tgatttatcg cgcctgttca gccgtcttgc
     5641 ccgtacaaaa caacttcgca aggccaaaaa ggcggatgtg ctggccattg ccagggcatg
     5701 gggtatcagt ggtgaggccg agctggccgt catgcaggct atcgctgaaa agccgggagc
     5761 gttacgcgtt ctgacacata cgcttaacca ggcgtggctc accgccagcg gtgaaggtgc
     5821 ggcgctgaca gaaaaacata ttaatgcggc ctttaaagag gtttatacca accctgaatt
     5881 actctcacag gtgtgattat ggcggtattt tatattcctg atatttacgg acgcttttac
     5941 ctggttaatt tcgataacgt gaaggtgatt tcactggccg aaaataaaga atgtggcgat
     6001 ttactttttg aatttaatga ccgcacacga atggtgatat ctgccggact tgatcgcgaa
     6061 ggtgcgacag aagtttacag cggaatatgc cgttctgttg gtgcgaaaca agtcagctaa
     6121 atgaggtgtt atatgaatat gcaatcctgc ggtaacaaaa tgaatttatt cgactccctg
     6181 aacagcgcgc gccgtctgac cgaacttgcc ggtgcggtac tggaacgcag taagcgctac
     6241 ccacaacgtt ttgcactgaa aaccacgccg ccggtaggca acgtgcaggg aaccggtgaa
     6301 attgaaatca ccatacagac caacggcctg cgccgccgtg tgaaggccac ccgcatcagc
     6361 ggctgcacgg tttactggga ggtgtgaggt gaaaaaaaat ctcattgcat gggcgtggtc
     6421 gagtggtctt attgagtttg gttacgtcct gccggaaggt gcattgccga tagttgccgg
     6481 aaagcctgcc acggtacggc atgtgattga ggttatggcg cgtcatggac gtgatgaaca
     6541 ggagcagtta ctggttccgg ggataccgga agcggtgacg gaggaagaag cctttaatgc
     6601 catgattcgg ttctgccgtg aggtcagacg ccgggtcagt tatccaaaca gaacgaggac
     6661 cagaggatga gtaaagtcgt acgcattatt ttcgaataca aggagcacgt tatccataaa
     6721 aacgctgatg gaacagtgcg catgggggta agtctggaca tacgttcaac cgggataaag
     6781 cagaaaggtg atggacccgc catgattttt ggagtggtta tgctcgcgga aagcagagac
     6841 tttgctgaac ttgtggcaat gaaagccaga gcgctcatga aagatatgaa catgagttcc
     6901 ggggttatta aaggtaatga atttaatcag caggggtaat tccatgagca aagtacgcgt
     6961 tatttttgaa tttgagcatg tttcgcatga cgaaaaaccg gcaggcaatg acagtgttga
     7021 agtgcatgaa aagattggag tggatgtgaa aacagaacgt gatacggata acaggccgac
     7081 gtcactctgt gacgtttatg caagcattct ccagtatcac agtcctgtaa ttattcagtt
     7141 tctctcagcg gaatttcagg catctgtaca ggcttttggg gcggatgcca tcattaaacg
     7201 ccaccgcgtg cataaagcat caggcacact gcaataagga aaaacaaaat ggctaaacgt
     7261 gttacaaaat taaaggccgc agccgaggct gcaccgcaga cccgcgaaga agtcagccgc
     7321 gatatccgca ccctgggcga tattcagcga gaggcgctgc ggctggaaac ggcgatgaat
     7381 gatgaagtgg cagaaatcac cgcccgttat acgccgcaaa ttgaaaacct taaaaaagaa
     7441 atcaaagtgc tttttaaggg gattcaggac tggtgcaaaa ccaaccgcga tgagctgacg
     7501 aacggcggca aaaccaaaac agccaatctg accaccggaa cggtgtcatg gcgactgggg
     7561 aatccatcat gcagcgtcag tcgtgatgtg gaaggtgtga ttgaaatgct gcgccgtatg
     7621 ggccttgagc gttttatccg cacgaaagag gaagtgaaca aggaagcggt cctggcggag
     7681 ccggatgcgg tgaaagggat tgccggtatt aaggtgaata aaggcgctga aagtttttat
     7741 gtcgagcctt ttgaacagga cgccggactg aataaataac accgcattaa tcaattaaat
     7801 atcacttcgt tttaattatg gcgctcgcgt caggggactg cctgcgcctg taaactgaaa
     7861 ataaggaaca ggatatatgg catattttta tttcaaatta gaccgtgtgc agacaaataa
     7921 atatttcacc aaatatcaac agactgtttt accgctacgc aacagtattc ttcgggcatt
     7981 actgaaaaat acaggcgctg ccggattgcg cttaaagccg tttgccatgg acgtaatcag
     8041 tgagttttat ttttctggtg ctctgcctgc gggctggcgt aagcgcgatg atgtggcttt
     8101 tatcggggac ggaccgtgct ttattgccag acctgatgag tcatgccctg aaggtccggc
     8161 gattgccgca atgattgaaa ccgctgaacg tgagttaaga aagcgtcctg atttccttgt
     8221 ctggctctgt gaaaagctgg gggtaatgag aatcccctcc atgtttaaca cggactcctg
     8281 gtggaccccg tcgctctccc gtgatgccct gtgcgtggtg tttaaagtag gcgcttatgg
     8341 cagggaaata aaagggtgta ttcctgaaga atgccaggaa attaaacatt ctgaatatgt
     8401 ggcgctgacg gaggaataat tcatgattga tgcaaaagtg cttgaagggg ttaaaaactg
     8461 gctgagtatt tacgggcgtc tgacctgcgg cattctggct gaaaaaatga atatgccgcc
     8521 atcctcgatg gtttattttc tgcgtgatgc ggtcgatgcc ggggtgctga cggaatgtaa
     8581 cggtttttat gatattccgc gtccccgccc ggtgcagccg gttcgtcgca aatgcagcca
     8641 ggaaggtgcg gctgatgatg ttcagtggtg cagcttcaga aaatccctgc cgtggattga
     8701 ggggcatgat attccgtcga tggcgtggga atttgctcag ggcgtactga cctgcgaaac
     8761 cgtttatgtg gtggctgaag ttgatgagca ggccatgaaa gaaggcgtgc cccagtttgt
     8821 gatggcgtat atcgacattc gcctgggtgt cattatctgc ggtttaagcg gctggaatat
     8881 caccgaacat gttctgcgtt acctgattgt tgaccggacg gctgcgcctg ccgggatatc
     8941 tgcggaggtg gcgtaatgtt ctttaaaaca tcaaaccctt ccgcgctggc tgcgtggcaa
     9001 aaataccagc aggactacaa gaaagttaag gatgaggcaa aacgtcttga ggccgtgctg
     9061 aatgttgatt gtcggtcggt atttgtatcc ggtatcaatg gtttttattt taaaggactg
     9121 cgttttacgg ataacaaata tccttttcat cgcgacttat ggcgaaaacc gactgcgtcg
     9181 aatggctgga gctgcacgcc gcgcacatcg tgtattccca aagccctgtg cgctgcctct
     9241 gacgaactta acagtctgtg gcgtgaatat tcgcccgtca cgtatgccag aaccgatgcc
     9301 ctgttgttct ggctgggtat tgatttctcg gcaatcctgt ttggccctgt ggagtggttc
     9361 tgcgttgacg atgtgattta ccttcagtgc ggcgtaaaac ccgcaaaaca gaaaatgacc
     9421 gaaattctgt ctgatgagtt ttatgctgct gaaaagcgag tcagggggtg atgtatgatg
     9481 attttacaac ccatggggcg aaaaggccgt gcacccgctc atgtccgtgc gtggacacct
     9541 gaagaagatg cactgctgat tgcgctttat ccatccaccc cggttaagga tattgctgtc
     9601 agggtaaaaa gaagtttctg gggtgtacat aaccggattg ttttattacg cggtacttac
     9661 ccggagttgc tcaaatgcaa acgtccaaga tttaaacatg atgaagataa atttatccga
     9721 aaaaatgcca gaacgatgac cggtaagcaa ataggagaat atctggggcg agaccgggat
     9781 tctgtccaca aacgggcgcg atatattggc gtaagcatga aaaaatacgg agagttgctg
     9841 cccttcaccc gtatacctga tgatgatgtt catcttattc gtgaattacg ggatgctgaa
     9901 tcaccacgac gtcttacctt ccgggaaata ggcgagaaat ttgaattatc cgagagtacg
     9961 gtgaatttta tttatcacca tcgccggact gccgaagatg ttgtattacg ggagttaatg
    10021 ccatgataac gacattattt gttgaatcag acgagcctct ggtatgtgtc gccggaatgt
    10081 cggtttgtgg cggaacgctg accggtgttt atttcgggga tttgcgcggt tatccctggc
    10141 attcactgga tgacgcattt ccgcctgata tggaggctgt cgtgctgatt gttcagtatg
    10201 gtgaccaaca ggaactgcgc atcggccata tggggtatga aggctttttt attgatgaag
    10261 aaaccggagc ctgccttgaa gatgaggacg gacaggtgac gcactggtgc catatttcag
    10321 ctttaccgga atgacgggag gtgaatgcgt gagaagttat attgataatg aaaaactgaa
    10381 gacaatcagc gactgtctta gcttgctggc aaaaataaaa gaaaccattg aagagattaa
    10441 atttcagctt gaatacgaac cctgtggcga tgatacatgg cgtaactctg ccagaaaagc
    10501 actagctgtc tttcagaaac aacgccggac tgttgaatac cgccttgctg tattgcgcca
    10561 ggaggaaaag gaaagaaata taagacgtca tgaactcgtt aatgattttc ttgttcgtga
    10621 attaaaggag cgtgtgccgg agtcggtatt ttttgagtgt gaggctgttg cccgtagcaa
    10681 agcactggaa actgattaaa caggcaggtg aatgatgagt attgaattaa gatcgtctta
    10741 tgaatatcgt aaaattctca tcgccggagg catgaaaccg gaagatgcag aaaaaatcgt
    10801 ttcttttatg gataaagaat gtgacaaacg ggatatgcca gaaattatta tggatgacat
    10861 gattctgcat tcagccgtag cgttaagccc gttatggatt gtgcattctc ttgctgaaat
    10921 tgccaaaggc accgataaac aggcagctgt tgctgccctt cagacgctta atgaaatgcg
    10981 tatatctcca cgcccgacat taatacatat gattctgtcc agcatggagg ataaagcaaa
    11041 tgaatagtct acccgctgga tgggcgcggc cactaatggc caggaagcat cattttttta
    11101 aaacaggcga aaatatcagt atatgtggac ggtggttata tctggctcat aatcgcgagc
    11161 cggatacatt tgaaagccct gatgactgtg ccgaatgccg cagaagagtg aataaggaga
    11221 aagataatgg acaatagttc tctgttgttc tggtgcttat atatcacatc ctttttcggc
    11281 gcatttgtta ttacccggtg gttatgtcga aaaatcatct gcttttttga taaaagacat
    11341 ccggttgaac gggcggctga tgcgctcatt cagcaggcca ttgtgttata tagcggtgaa
    11401 tttttctgcc ggataacaac cagagatggc tggcacataa tgattattcc accaacacac
    11461 catgcccggt gggatgaggc agaaaaggct ttccatgtcc gtaaaaaggt aaatacggta
    11521 tgagaggaaa actgatatcc gccattcatg tggcaaagcg cgagcttgcc ctggatgatg
    11581 agacttatac atccgcgctg ctggcagcca ccggcaaaac cagctgccgg gatatgtcac
    11641 cggatgagtt atcccgcgtg ctggatgttt tcaaaaaacg cggttttaaa gtgcgtcaga
    11701 acccggttaa ccgggcctta aaaccgggta cggtgaccgc caaaattcgc gccatctgga
    11761 aggtgatgct ccggcagggc tttatcaccg atggtgcgga aaccgccctt aaccgctggg
    11821 tgaaatcgca gacggccgcg cagaacggcg gcgaaggtgt ggcaaactgg cagtggctgg
    11881 agcaacaccc cgccctggcc tcagatgtgc tggagcgtct caagcgatgg caccgccgca
    11941 aaatgctggc cgcgatggga atgcccgaac gcacgctgat ggggtatgac gccgtttgca
    12001 ggcagtatga aaaatcactt ccccgttaac cccaaatccc gccacaacgc gggattttta
    12061 ttttaaactt accgggaacg tgagaaccgg aggctgatat ggcagaaact cagatgagca
    12121 tgtttggtgg tgacagtgag caactgcacg cccttatcga ccgccttgat gacatccctg
    12181 atgatgttct caaaaagaac tggccgcgga ccctgtcaga actggttgaa gtcaccggcg
    12241 cagaacttca gcgtcagggg attgagccgg tactggccgg taaactggca cgcaaggtgg
    12301 ctgcggctca ggcagcctat atgggcggac ggggttatta cctgccggtc ggggaatctc
    12361 tctttgccga gctgcgaaac aatgagatat tttcgcgctg ggaccggggc gagaaaattg
    12421 aatcccttcg ccgccattac cggatgtcag aaacccagat ttataccgtc atacgtgaac
    12481 agcgccgtct gcatctggca agaacgcaac cgccactttt ctgatattcc gcaaagcctg
    12541 ccgcctttcc ttcaccgtta cgctgactca gagaacatca tgagacagcg taacaatgcc
    12601 aaaactcccc gcaccactgc gtaaaaagct gattgccctt gttctggccg gtgccgggac
    12661 gttcacgatt gccacgcatt acaccggtta ctgggaaggg aaagaaaact ccacgtatat
    12721 cgatcccacc ggtacaccca ccatctgtta cggccatacc ggcccggatg tgaaaccggg
    12781 tatgaccctg acggatgaag aatgcctgga actactggaa aaggacatga aatgggcctt
    12841 tgcggccatt gatcggcgtg ttcaggtgcc gcttacccgt ggtcagactg tggcgctggc
    12901 ttcgtggatc ttctgggccg gtgaaacgaa cttccgcaac tccacgcttc tgcgcctgat
    12961 caatgccggg cagatgcccg cgtcctgtaa gcagtatatc cgctggattt attcaaaggg
    13021 ggtgaaactc cccggccttg aggcccgccg ttcggcggat gaatggttat gtcgctacga
    13081 cttgccgaaa gtctgaaccg cttctggcga ccgctcatga tagcgctgct gtgtgcggta
    13141 ctgctgttac ggggtgtcct gtggctgcgg tgaataatgc cttaccggcc ctgattgccg
    13201 gggcgtgcat ggcggcgctg gggattatcg ctgtactggt tgccgcgata tggggaatgc
    13261 accagaagac gcagcgcctt gaagacaata atcaggtgct tgtgcgtgag cgggatgagg
    13321 cgcgtcaggt gctggcaaat cagcagcaca ccctgcaact catttcacaa atcagtgagg
    13381 cggccacgaa tgaaaaacaa cagaacattc agcacagcga gggacagcag agcgttgtcc
    13441 gccggtcgct ggcagcggtg cctgcggcct ctgcccctgt tcctgatgat gtggctgatc
    13501 gggtgcgccg ggccgtctgt gaaatacgtg cctgtgaagc cggtgccgat ccccgctgaa
    13561 tggctggctg actgcctggt ccctcctgtg ccggagccgt tcacgtttgg ggcatcggtc
    13621 acttacaacc tgcaactgct ggcggtgatc aagaactgca acgtggacaa ggccagtatt
    13681 cgtcgtctgg aggcgcggcg acaacatgaa tttactgata tggccggaac gcctgctgtt
    13741 ccggcaggaa agacgaagta aggaaaaagg tatggatgat tcagatcgtg ctcaggctgt
    13801 gatggagcgg ggatcagaac gcgccctttg taaccggctg acacgtaagc gccgggcaat
    13861 ggatgctccg ggcagacgtg tctgcgcgga ctgtggcgga gagatccccg ccgcacgtct
    13921 tgttgctgtg ccggatgcca tccgttgtgt gaactgccag aacatcatgg aggcccgtca
    13981 tgtgggtcag catcgttaaa gattatgttg tgccgatcct ttcggcgacg gcaaccgctg
    14041 gcgggatatt catggcgctg atgcgcaaaa cgtttgtccc ccgcgaggcc tttgaaaaac
    14101 tctccgaccg cgttgagaag gtggaaaccc gcctgtcgtc actgccgaca gaagctgagg
    14161 tcaaccgtct gaatgtggaa atcgtgaccc tgcggggtga actgaaaacc acgaacgcca
    14221 cgctccgctc tgtctcctat cagaacgaac tgctgctgga gcaggcggta aggaaaaaaa
    14281 cgaatgagta atttcattac tgaagatcag cgtctggtca ttctgcggtc gctggcagat
    14341 tacaacggtg aactgggtga atccgtgctc caggactgtc tggatgatta cggccaccgc
    14401 gtgtcccgcg acaccgttca cacccacatt gcctggcttg ccgagcaggg actggtgcgc
    14461 aaacgcgttc tgattaacgg ttatttcatc gcggaactga ccggccgggg gcaggatgtg
    14521 gcggaaggcc gcgccagcgt tccgggtgtg aagaaaccac gcgcaagggg gtaacgatgg
    14581 acaagccgac gcgtggacgc gtgcgcaagg tggatttgct ccccgacagc atccgtaagc
    14641 cactgctgga aatgctgcgt gaaaaacgtc tgacgcaggt ccagatacgc gaggaaatca
    14701 accgtctgat ccgtgaggcg ggcctgccgg aagagcagca gctttcaccg gcggccatca
    14761 gccgggaagc ctcccgtaat gagctgattg cccgtaacct gcgtgattta cgcgagcaga
    14821 caaaagccat gatggccgaa ctgggggaca agccaaccgg tgaaaccact gcattgatcc
    14881 tggagatgtc ccgcgccctg atgtaccgcc gtctgcgtgc cgcgaccgaa tccctgaaca
    14941 gtgacagcga cgtggatatg cgtcttatta aggacatcct cctgtcagca cagcgtgcgg
    15001 aaagtgccgc cgagcgcagc attaagcgtg aaaaagaaat ccgggccgca tttgctgaag
    15061 agatggcaaa cgccgtcacc gacgagctgc gcggcgtgga cgggatgagt gaacagcttg
    15121 agtcccgtat taaggggatt ctgttgggta aagcctgatg aataacgccg aagaactctc
    15181 ccccttactg acgaataccg tctccacccg caaaattgat ctggccggtg aaaaagccct
    15241 gctgggcgtg gatgtgccgg acagccttga tttgcccggt gatatgccgg tatttctgga
    15301 ttatcaggcc cgctggtttg aggatgaaag cgaggtctgc atcgcggaaa aatcccgccg
    15361 caccgggctt acctgggcgg aagccgggcg taacgtcatt accgccgcaa agcctaagcg
    15421 gcgtggtggc cgcaatgtgt tttatgtggg gtcaaagcag gagatggcgc tggaatatat
    15481 ttctgcctgc gcactgttct cccgtgcctt taaccagctg gcagatgctg atgtgtatga
    15541 gcagaccttc tgggatcggg ataaaaaaga agaaattctg acctacatga tccgctttcc
    15601 gaacagtgga ttcaaaattc aggcactgtc ttcccgtccg tcaaacctgc gcggccttca
    15661 gggggatgtg gtgattgatg aagccgcgtt ccacgagtca ctggatgagc ttctcaaggc
    15721 ggcaatggcg cttaccatgt ggggcgcgcg cgtgcgtatt atctccacgc ataacggcgt
    15781 cgataatctg tttaatcagt acattcagga agcccgcgaa gggcgcaagg attacagcgt
    15841 ccaccgcatt accctggatg atgccattgc ggacgggctg taccgtcgta tctgttatgt
    15901 caccggccgc gaatggtcac cggaaagcga gcagaagtgg cgtgatgatc tctacaaaaa
    15961 cgccccgacc cgtgaggatg ccgacgagga atacggctgt atcccgaaaa aatccggcgg
    16021 tgcctatatt cctcacgcgc ttattgaaat ggcgatgatc cgcgacatcc cgattctgac
    16081 gtttgaagcc ccggacgact tcatcagccg tgccgcatgg ctgcgtgaat cggaggtttt
    16141 aacctggtgt gaagaacatt taaaaccgct tttagaggcg ttaaattccc gttcccgctt
    16201 cagcttcggg gaagactttg cccgcaccgg agacctgtcc tgttttgtgc tgctggaaat
    16261 cactgaatcc ctggcaaaac gcgaggtgtt tcgcgtggag ctgcgcaacc tgccgtatgc
    16321 ccagcaggag caggtgatga tgtacatcct gacccgcgtt ccggcgctgg tcggtgcggc
    16381 gttcgacgcc accggtaacg gcggctatct ggccgaagcc gcgctgctgg cctttggccc
    16441 ggacatcatc gactgcgtga tgttatcgcc gaagtggtac tgcgagtgga tgccaaaact
    16501 gaaggccgag tttgaggatc agaatatcct cgtcgcccgc catcagacca cgcttgatga
    16561 tttgcgccat gtgaaggtgg tgaacggtat cccgcaaatt gacaaggggc gtacgaagga
    16621 ccagaacgca acggctgtga acgcccgccg ccatggcgat tttgccgtgg cgctctgtat
    16681 ggcaaaccgg gcgtcataca tggagggctt catcctggat gaatcagcct gtcaggcgct
    16741 gccggagcgg tcgcgggcca tggagggcgg ttatcgtgat gacgatgagg catatcatga
    16801 atttgatcgg gggtgctggt agtgggacgt ataattgatc ttgacggaaa acctttctcc
    16861 tttgacccgg agatgcagag tgccgcgctg gatattccgc agattgccag ccgttatatt
    16921 gaacatccgg cctcgggtat caccccgaac cgggcggcac agtgccttcg cggggctgaa
    16981 cgtggcgatc tgattgccca gtccgatctg gccgctgaca ttgaagaaaa ggatacccat
    17041 ctttttgcag agctgggcaa gcgacgtctt gccattcagg gggtgccctg gagtattgag
    17101 ccgcccccga acgccagcgc gaatgagaaa aaggacgcgg aaatgctcga cgaatacctg
    17161 cattccgccg actggtttga tgccatgctg tttgatgcca ccgatgccat cctgaaaggc
    17221 tattcctgca tggagattga gcacgggatg ctcggtaaaa tgcacatcat ccgcgccatc
    17281 cgctggcgtg acagcgggca tttctgcctt aacccggatg atttgagcga actgcggctg
    17341 cgtgacggca gccatgccgg ggtggcgttt cagccctttg gctggatagt gcatcagtca
    17401 cgttcacgca ccggttacgg cggcgcaaca gggcttgtca gaacgcttat ctggccgttc
    17461 attttcaaaa actattccgt gcgcgatctg gctgaatttc tggaggtgta cggcctgccg
    17521 atgaaggtcg gtaaataccc gtccggggca acaccggagc agaaaagcgc cctgatgcgg
    17581 gcggtgatgg atatcgggcg acgtacaggc gggatcatcc cggccgggat gtcgctggag
    17641 tttcaggcag ccgcgaacgg tcaggccgat ccgtttgaaa ccatgatttc gtggggggag
    17701 cgttccatct ccaaagcaat actcggcggc acgctgacca cggaagccgg agacaaaggc
    17761 gcgcgctcgc tgggtgaagt gcataacgag gtgcgccggg aaatcaggga ttctgattta
    17821 cgccagctgg ccgccacgct gaaccgcgat ctggtgtatc cgctgtatgc cctgaacacc
    17881 gcccacgcca ttgatatccg tcgcctgccg cgtatctgtt tccagacaaa agagccgggg
    17941 gatatcacca aaatcaccag tgcggtgatg cagctcagta cggggatgga tattcctgat
    18001 ccctgggtac gggagcagac cggtattcca cagcctgccc ccggtgaagc catcttccgt
    18061 gtccgtcaga gtggcaatga acctgctcag acagacaaag agatgccacc ggaaaaacag
    18121 gagaagacag agcagacagc gctggctgcc agactgccgg aagcgaagag cagcccccga
    18181 gatgaactgg acgacatggg ggatgcggtg cctgcccgcc ggttacagga ggccatcgac
    18241 ccggtactgg agccggtcat tgaagccatc agaacgcggg ggctggcgga ggcactggca
    18301 gacctgcctg ccctttaccg tgaaatggat gattcccgcc tgatgacgct gctcagtgat
    18361 gccatgtttg ctgcggaaat gaaggggatg ctggatggca caggggattg atttaggtta
    18421 tgccgccacg cttccctcaa aagaggcggt ggcatacttc cgcgccaaag gggcgcatat
    18481 cagctggaac tggttcgaaa caaacgcaga tgttcatgcc cgttcattca cggcggcaaa
    18541 agtggcacgc ctggacgtac tgaccacact acaggcggaa gtgcagcgtg ctgttgatga
    18601 ggggatttca cagaaagcat ttatccgcac actgacaccc cgcctgcaaa agctgggatg
    18661 gtggggaaaa cagattgtgg tggacagtgc cggtaacgcg gaagaagtgc agctgggcag
    18721 tccccgccgt ctggcgctga tttacaacgt gaacacccgt gtggcttaca atgccggaga
    18781 tgatgaacaa cacggacacg catccgttct ggcagtatgt ggcggtgatg gacagccgca
    18841 cccgcccggc gcattccgcc cttaacggtc tggtattccg ctatgacgat ccgttctgga
    18901 atacacacta cccgcccaat ggctggaact gtcgctgccg tgtccggcca ttgtctcagg
    18961 cccgtctgga tgcgatgggg ttatccgttt catccggtca ggatcatctc tccacccgca
    19021 atgttgaggc tggcgtggat aagcagaccg gggaagtcag agaaatgccg gtgaccacat
    19081 attcagatgg caccagaacc atgacaccgg atgtgggctg gtcatataac cccggttcgg
    19141 cggcgttcgg cacagaccag gcgctgatcc gtaaactgat cgaggtgaaa agcccggcgc
    19201 tgcgggaaat ggtggttcag gagatgaaca acagcccgga gcggcaactg gcgttccgca
    19261 tctgggcaaa aaacatcatg aaaacccggc gaggcggtaa cgatatccgc acgctgggct
    19321 ttatgaccga aagcattgcg caggcggtgg aaagccggac gggaacgcca ccggcccgcc
    19381 tgctggcgat gagcggtaaa aatgtgctcc atgcggacag catgaaacat cagaatgacg
    19441 gtatcgccct gacgccggag gatttcgggc gcttaccggc aatgctggca aggccaaaag
    19501 cggtactgtg ggacaaacgg cacaacaatc tgatgtatat cgtggaaaat caggatggca
    19561 gtgtgcagat tgccgttaat gcaccgtaca gcctgaagcg tcagccggat aaactggatg
    19621 tgattgttaa tgcttaccgg gttatcaata tggataagct gaaatcggat atccggggcg
    19681 gaatgcttga ggtactggaa ggagatattg attaacggtc agtggcgggg gtcgaacccg
    19741 catacatgat ccacccggag gcaggcaccg actttaccag ttaagcgtac actgaccgcc
    19801 tgtttatttt atattcatcc tttatgcgga ggcaatatgt catccattga tgccgctgtc
    19861 gttgttgacg ttacgcgtct ccagcgggtc tttgcccggc ttcagtttgt gggtggtgga
    19921 aaagacctga cccgcagtgt ggcgtccagc cttctgtcat cgtcagaaat ggcgtttgaa
    19981 caggaaaaag agccggacgg cgaacgctgg catgactggt cagatcctta ccgcaagtgg
    20041 cgtacccgta agggatacat gcccggcaaa atcctgacgc tgaacggcga tctggcccga
    20101 cgcctgacca cggattatgg cgatacctgg gcgctgattg gatcaaatga gccttatgcg
    20161 gccattcatc agtggggggg cctgccaggt atgccgcctg gaccggcggc cattggtgca
    20221 cgtccgtata tgggctttga tcaggtggca gagcaggaga tcatggacga aatcagaaaa
    20281 cgctttaaaa aggccacaga aacgccttaa ttgtttaagg tatgcaaatg cattacctta
    20341 cccccttcag gcgcgtgtcg tgattttcta acctgaattt aacgggcttt aaaatctgcg
    20401 tgaagcgcct ttgtattttt cccctgaaag cacttcctga aatcccgcaa aacccgccct
    20461 gaaaattccc ggcctatgct gccggaatga agacgaaaaa cacgcccaaa cttgcttatg
    20521 ccattctgaa tgccatcagc ctgtccgcag acggggacgg tgactggtgt cagatcatgc
    20581 cagccggtcg ggtaaaggcc cgtgacggtc gcccggaaaa accggccgag ggctggctga
    20641 tcaaccatgc agccgttgag cgcatggtct cccgtgttgt ggcgctcaat cagccggtga
    20701 aaattgacta caaccaccag accctgattg agggacatcc ggcaccggct gccggttttg
    20761 tgatggcctc accggagaat tttcgcttca gtgaagagcg aggtttcgag gtgcgcccga
    20821 agtggaaccc tccggccctt gaacatctgc gtaataacga attcccctgg ttttcaccgg
    20881 tgattggcta tgacgagagc accggcgaac ctgtcgaact tcggatgctg gctattaccg
    20941 gtgaccccgg tctgaccggc atgaatcctg tcgccgcact gtcggcggat gacctttata
    21001 acgccttaaa ccctccttta aaggatacct ccatgaatga gcaattacgc cagttgctga
    21061 cggcgctcgg tctgaccgtg gctgacggtg acgaatttac gccggagctg ggcacggcag
    21121 cgctgtctgc cctcaccggg atcaaaaccc gtgcggatgc acacgacaac ctgaaaacac
    21181 aggtcgccag tctgtcggca gagctggaaa ctgcaaaagg cacaccggct ggcggcagta
    21241 ttgacctgac gaaatacgtg cccgttgaaa cgtataacgc cctgcgtacc gaatacgttg
    21301 cgctgtcggc gcagcacggc agcaccacgc tggagcagtt gctggacaag gccgagtccg
    21361 aaggacgcat cttcaaaagt gaacgcggct atctggaaca actgggcggg cagattggcg
    21421 tggcggcact ttctgcacag cttgacgccc gccagccggt tgcggccctg accggccttc
    21481 agaccgacac cgtgaccgtg ccggataaaa agactgccac cgctgtgctg tcggctgaag
    21541 atatcgcggc cgccaaactg ctgggtaaaa ccgaagccga gttcctgaaa atgaaagagg
    21601 aaatgcaata atgcctaccc cgattacacc ggcgatgatc accgccctga tgacgggcta
    21661 ccgttctgat tttcaggccg ggatgtccat ggccccgtcg cagtacaaaa aaattgcgat
    21721 gaccgtgccc tccacatcga aatccaacac ctacggctgg ctggggcagt tcccgcagtt
    21781 ccgtgagtgg atcggctccc gcgtcatcga gaagatgaaa gcctatggct atgccatcgt
    21841 gaacaaaact tttgaaggca ctgtcgccat tagccgcgat gacttcgaag acgacaacct
    21901 cggtatttat tccccgttgt tccaggagat ggggcgcgct gcggcggtac aaccggatga
    21961 gctggtcttt gctgccctgc gtgacgggat cagtgcggcc tgctatgacg gtcagaactt
    22021 ctttgatacc gaacatccgg tttaccccaa agtggatggc tccggggatg cgcagatggt
    22081 cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca caggctgatt acagcggtcc
    22141 ggcctggtat ctgcttgact gctcccgcgc ggtaaaaccg ctgatttatc aggatcgccg
    22201 taaggctgaa ctggttgccc agaccaaagt cgatgaaggt cgcgcattca ccgataacga
    22261 atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac ggcttctggc agatggccta
    22321 catgatgcag tcaccgctga cgctggatgc actgtggcac ggctggtcag ccatgcgcga
    22381 atttaccgct gacggtggcc gcaaactcgg cattaaaccc acccatattg ttgtccccac
    22441 ctcgctggaa aaacaggcgg tgcagctgct ggagcgtgaa ctgttcgcag acggaaacgc
    22501 aaccgtctct aacgagatga agggcaagct ggagctggtt gtcgcggatt atctgtaaac
    22561 ggtgagccgg gcttgcggcc cggcccctgc ggagggcaaa tatgaatgaa catcacactg
    22621 tggcagcaac agatgattca ggtcttcagg tcagtggtga caattctgtc acggtgctgg
    22681 cagaagtgcg ttgcagtcgt tcggcgtttc gtcgtgcggg gtttctgttc acgcgtggac
    22741 gtcagcaggt tgaggtcacc ccggagcagc ttgcccgact ggaggcggag ccatgcctca
    22801 ctgtgcgaat actacagacg tctgctgatg atgcggggag cgtggcgggt gtggttcatg
    22861 cagtggccgg tacagattta gccgaagccg aagccgaagc cgaagccgaa gccgaagccg
    22921 aagccgaagc cgaagccgaa gccggacagg atgctccctg gaaaaagacc ggtaacaaag
    22981 cgaaacaggc ccgtgcatga attacgccac tgaaaccgat atgcgggcgc gttaccgcga
    23041 ggatttgctc aggccgttac tggctgtgcc ccgttcggat gagccggata cgcgcaagct
    23101 gaaccgggcg ctgacagatg cgtcagccct tatcgacagc tatctgtccg cccgttacac
    23161 gctgccgctg gaggttattc cggccgttct tgttcagcac tgttgcgcga ttgcctttta
    23221 ttacctgtgc gatcagcgag cctcagatca ggcgcgtgac cgttaccgtg aggcgctggc
    23281 ctggctgaaa gatgtcatga acggcaatgt gccggtaggt gtggatacga acggagcggc
    23341 ccctgaatcc ggggatttac cacaggttca gtctgatgca gcggtattcg ggcgcaacca
    23401 gaagggcttc atatgattac ggaaaccgaa caggcataca tcgcccgtat ccgtgagtat
    23461 ttcgggaatg aactggtgtc tgttgacacg catcccggcg actggaacga cagcgtactg
    23521 cgcaccatgc tgattaacgc cccggcgatc tacgttgcct ggctgggtgc cggtgaaggc
    23581 cgtacccgtg gtcgcctggt cagtcactgg gtgttctacg tcatcggcga catgctcaac
    23641 gggcgtgagg ccagccgtcc cggattgtat cagattgtgg cccggctgat tgccgtgctt
    23701 aacggcttca gaacagaaaa aacctcaccg ctttactttg aaaaggcggt caacggttac
    23761 accgaaaccc aggcggacag tggtgcggtg atgtatgcgc tttatttctc ctgcgaggaa
    23821 atgatcgcac cgctgaccga tatcagctcg ctggacgact tcctgcgtca ttacgaaacc
    23881 tttgtggagc cgcccggtac gccgccgttt gaggcacata ttgatttgcc ggggaacacg
    23941 acaacggcgg tcgaaccgcc ggaggaactg taaatgaaaa ccatctttat caaacccgcg
    24001 ccgggacgcc tgattcgtga tcccgacaca atgcgcccgc tggcacagga gggggaggaa
    24061 aagcccttta cgccgttctg gtgtcggcgt cttgatgacg gcgatgtcat tcaggctgaa
    24121 aaagccgctg aagaggctcc ggctgtatct gcggatgcaa cgactgcgac agaaaaaccg
    24181 gtcgcccaac cggcatccga taaggagaaa ccgcaatgat taattttgac gggatcggta
    24241 atgaccgccg tataccgctg attgaggttg agtttaataa ctccatggct gtcaccggca
    24301 ctccggcaca gcgtcagtgt gtgctgctgt ttggtcaggc gagaatgaaa gagaatgccg
    24361 tggacggtgc cggtgttctg gatgttccgg tcaggatcac ccgtgcgtct caggcaacag
    24421 aattctgggg gcgcggctcc atgattgccc tgatggtggc ggaatttatc gccatcaatc
    24481 ctgatgctga actgtatgcc attgctcagg ggagcggcac cggtcaggct caggcatgtc
    24541 tcatgaatat aattggcacc gcctctcagg atggtgttct gcacatttat atcggcggtc
    24601 ggcgttatct gctggctgtc acgaaggggc agaaaggtaa ggagcttatt gacgctctgg
    24661 tctctgttat taatgccgat gctgatgcgc ccttcagtgc ttatgcaggt caggtagcag
    24721 ataacgccag cggactcaag ggaagtatgg gaattaatgc ccgttttacc ggtgaatgtt
    24781 ccgtacatga tatccgtgtt aattatcatg acggagaaac cacaccgggt ggactgacca
    24841 ttgaacttgg gtatcccaaa acaaaagcca ctaacccgga tatcacccga agcgtggcgg
    24901 gtatgggcga tcgccagtac aactatgtcg tcatgcccta taaggatgat gcaaacctga
    24961 aaatcatcag tgatgagctt ctgaaacgct ggggaccggc caaaatgtct gacggtgtgc
    25021 tgtggatggc gcataccggc acgtttggtg aggtgcaggc attcggggca aaacgcaatg
    25081 attttctggc gacctgcaca ttcattccca aagcccctga accggattac ctctgggcag
    25141 cggcggtctg cgccacctgt gcgccgtctc tgggggcaga tcctgcgcgt ccgcttcaga
    25201 cgctggcgct tccgtcacgc atggccccgg ccccggccga tcgtctgacg cgtgaagagc
    25261 gtaacagtct gctgtgggga gggattgcca cggtcagtgt ggctgccggt gatgtggttc
    25321 agatcgaacg tcaggtgacc atgtatcgcc agaacgtgta cggcgaaagt gatcccagct
    25381 atctggatgt ggaaaccatc tacaccctct cctggctgcg ttattccctg cgtactttta
    25441 tcacacaacg ttttctgcgc cataaactgg ctgatgacgg gacgcccgtt cgtgccggtc
    25501 agaatatcgt gacgccggag atcatgaagc ttcagttaat ttcgctgggt gaagagtggg
    25561 tggaactcgg gctggtcgaa aatctggaca cctttaaaaa gaatctgctt gtggagcgta
    25621 atgcgtcaga tcgaaaccgt cttgacgtgt tatgcacgcc ggatctggtt aaccagttcc
    25681 gcttcctggc agcacagatc cgtttcattt tgtgaggtaa ggcatgagcg gaaaacagta
    25741 tcagggcacg gccactatcc gtgtgaacgg tcaggaatac gagacgcttg agggggctac
    25801 attttctccg tcaggttttg aacgtgaagt ggtgaaaggc gcgaaagtct atggttaccg
    25861 ccagaaacca cgtgaagcga cactggactg caaatttccg gcaggcggag aaggttcacc
    25921 agccgccgat gaaatcaaca actggactgc tgtcactatc gagtttgtgg cggataccgg
    25981 cgaagtccac atgatgacga aggcctggag cagtgaaccg gcctcgcttg acggtggcgg
    26041 ggagatctcc gtgaagttcg ccagcgccac cagtacccgt gttcagtgat caggaaaaat
    26101 aaaatgacca cacgtaagaa aaaaacggca gtttccgagg cagccgtgat ggaggcaatc
    26161 cgggaagcac ttgagggggc tgatccacgc actgccgggc tgacagagca actggcaaag
    26221 ggatatgtgg atctgcttga tggtctgccg tttggtgaga cccgtgaata tcgtgtcacg
    26281 ttccgggaac tgacagcgaa agacagtatt gacgcagaag cagaagccga gcgcgtggtg
    26341 gagacaaaca atggcccgat gctgatagcg tctccgtcgt tacgcggtgt tgcactgctg
    26401 cgccgtcaga ttgccgcagt gggagacatt gaaggtccgt tgtcaccccg tcagattgga
    26461 cagttaagcg agcgcgatct ctcccgcctg atggcagcgg tcagcctgct ggataccgcg
    26521 cttgccggaa agctggcagc tgaccggggg cgatcaggcg cagtgtcggg atcagattga
    26581 agaagcggca attatcctgg ggatggtgac aaaaagcggt ccggaatggg cgcttaacct
    26641 ccccctgtcg caactttacc ggcactgccg acagacagaa aaaatcatca gaacgaagca
    26701 gtaagtatgg cccgaaatct cagagcatct ctgatagttg atttgctcgg caatatctca
    26761 gccaaatccc gccagtggtc acaggagctg ggggcattct cacgctccgg ccaggcgggg
    26821 ctgggtggcc tgggaaatgc tgcccgccgt gccgggcagg aaacagatgt actcggaagc
    26881 aggatgcagc gcacgctggc cggggtgcgg ggcagtatcc gccatgtcac ttctgatttt
    26941 gaccgtcttc agggcagtat taccggcacc atcgggcgga tcagcaatct ttacggaatg
    27001 ctggccggtg gtgctgctgt atatggcttt aaccggggat ttattcgccc ggcagcggag
    27061 atggaaacct atatgattcg cctgaactcc ctttacaaag gggatcgggc gaaaactgat
    27121 gatgtccgca ggtgggcaat acagaatgcg aaggaaacca cctgggggct ggctggcgtc
    27181 atgcaggaat atacgtccag tctgggcttt ggtatgagtg acagggaggc ccgaaatttt
    27241 gtcaccatgc ttcaggatca gggtgccgtg ggcggctggt cactgtccga tgcacagggg
    27301 gcatccctgc aactgaaaca gatgtatgca agaggcagta ttcaggcagc ggatgcaaat
    27361 attcttgcca cctacggcat taatgcctac cgcgtgctgg ctgatcgtct gggtgtggac
    27421 cagaaagtgg tcagaaaact tggtgaaaaa gggctgcttg ggccggacag tattcgcctg
    27481 ttatttcaga cgctggcgga acaggccagg ggagcacaaa aggacgcaat gaattcctgg
    27541 agtggcctga cggccatgat gggtgacgtc tgggatcagt ttgcccggga tgtgatggac
    27601 agtgggccat ttgaaaaact gaagggtaat ctcaggggat ttctttcatg ggttgacagt
    27661 gccaaatctg atggcagtta ccattccctg gcggaaagta cggcatcagc catgaaccag
    27721 ggctttgagt atgcccgtga tgcagtgaca gggttttatc aggcgatcag aaaggtacgc
    27781 gacacgcttc aggctctgcg ggatgcgggc tatggtgatg cgctggatcg cattggtcag
    27841 ggggcacaga ccgccgcgaa atacctgctg tacatgtatc tcgccacccg tgccctgaaa
    27901 atggcgcggg ctgtcggaac ggggattata cgtccgggtg ctgcattgct gggatacgga
    27961 ttatcagcgg cggccttcct tacctcacct ttccggcgct ctcctccggc cggaacacca
    28021 ccaggcacat ctcagggacg agggcaacgt tttataaact ttcttaccgg ggtaaatccg
    28081 gcagccgttc agccagtact tgtaacaaac tggcccacag gaggactgtc cggcaccggc
    28141 gcgaccgata catcctcagg cggacgtcag gggcggggcc gcaggaaacg cggaccgggg
    28201 cgcggaagac cggtcacgcc tcccctgcca ccgtccccag tgccaccgtc tccctctt
//
LOCUS       DAZFFI010000056        28183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFFI010000056 DAZFFI010000000
VERSION     DAZFFI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.056"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..860
                     /locus_tag="ACHJ12_002818"
     CDS             <1..860
                     /locus_tag="ACHJ12_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4990580.1"
                     /translation="LMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLE
                     SIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRI
                     QVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNSQLTSQHQYPTNPS
                     ESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSD
                     KIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSS
                     GGGGASGRW"
     gene            complement(920..2218)
                     /gene="eno"
                     /locus_tag="ACHJ12_002819"
     CDS             complement(920..2218)
                     /gene="eno"
                     /locus_tag="ACHJ12_002819"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4990581.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2306..3943)
                     /gene="pyrG"
                     /locus_tag="ACHJ12_002820"
     CDS             complement(2306..3943)
                     /gene="pyrG"
                     /locus_tag="ACHJ12_002820"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4990582.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4171..4962)
                     /gene="mazG"
                     /locus_tag="ACHJ12_002821"
     CDS             complement(4171..4962)
                     /gene="mazG"
                     /locus_tag="ACHJ12_002821"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4990583.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5033..5368)
                     /gene="mazF"
                     /locus_tag="ACHJ12_002822"
     CDS             complement(5033..5368)
                     /gene="mazF"
                     /locus_tag="ACHJ12_002822"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4990584.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5368..5616)
                     /gene="mazE"
                     /locus_tag="ACHJ12_002823"
     CDS             complement(5368..5616)
                     /gene="mazE"
                     /locus_tag="ACHJ12_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4990585.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5694..7928)
                     /gene="relA"
                     /locus_tag="ACHJ12_002824"
     CDS             complement(5694..7928)
                     /gene="relA"
                     /locus_tag="ACHJ12_002824"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4990586.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7976..9277)
                     /gene="rlmD"
                     /locus_tag="ACHJ12_002825"
     CDS             complement(7976..9277)
                     /gene="rlmD"
                     /locus_tag="ACHJ12_002825"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4990587.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9334..12090
                     /gene="barA"
                     /locus_tag="ACHJ12_002826"
     CDS             9334..12090
                     /gene="barA"
                     /locus_tag="ACHJ12_002826"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4990588.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12322..13662)
                     /gene="gudD"
                     /locus_tag="ACHJ12_002827"
     CDS             complement(12322..13662)
                     /gene="gudD"
                     /locus_tag="ACHJ12_002827"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4990589.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVARLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13683..15023)
                     /locus_tag="ACHJ12_002828"
     CDS             complement(13683..15023)
                     /locus_tag="ACHJ12_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO4990590.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15025..16377)
                     /gene="gudP"
                     /locus_tag="ACHJ12_002829"
     CDS             complement(15025..16377)
                     /gene="gudP"
                     /locus_tag="ACHJ12_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4990591.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16812..17261)
                     /locus_tag="ACHJ12_002830"
     CDS             complement(16812..17261)
                     /locus_tag="ACHJ12_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4990592.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGLQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17279..18061)
                     /gene="truC"
                     /locus_tag="ACHJ12_002831"
     CDS             complement(17279..18061)
                     /gene="truC"
                     /locus_tag="ACHJ12_002831"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4990593.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFNAPSGQDGEISS"
     gene            complement(18061..18390)
                     /locus_tag="ACHJ12_002832"
     CDS             complement(18061..18390)
                     /locus_tag="ACHJ12_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4990594.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19012..19557)
                     /gene="syd"
                     /locus_tag="ACHJ12_002833"
     CDS             complement(19012..19557)
                     /gene="syd"
                     /locus_tag="ACHJ12_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4990595.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19625..20473
                     /gene="queF"
                     /locus_tag="ACHJ12_002834"
     CDS             19625..20473
                     /gene="queF"
                     /locus_tag="ACHJ12_002834"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4990596.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20585..21949
                     /gene="ppnN"
                     /locus_tag="ACHJ12_002835"
     CDS             20585..21949
                     /gene="ppnN"
                     /locus_tag="ACHJ12_002835"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4990597.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22506..23795
                     /gene="sdaC"
                     /locus_tag="ACHJ12_002836"
     CDS             22506..23795
                     /gene="sdaC"
                     /locus_tag="ACHJ12_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4990598.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23853..25220
                     /gene="sdaB"
                     /locus_tag="ACHJ12_002837"
     CDS             23853..25220
                     /gene="sdaB"
                     /locus_tag="ACHJ12_002837"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4990599.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25332..26087
                     /gene="xni"
                     /locus_tag="ACHJ12_002838"
     CDS             25332..26087
                     /gene="xni"
                     /locus_tag="ACHJ12_002838"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4990600.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26142..27290)
                     /gene="fucO"
                     /locus_tag="ACHJ12_002839"
     CDS             complement(26142..27290)
                     /gene="fucO"
                     /locus_tag="ACHJ12_002839"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4990601.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGVGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDTVELYHTAW"
     gene            complement(27318..27965)
                     /gene="fucA"
                     /locus_tag="ACHJ12_002840"
     CDS             complement(27318..27965)
                     /gene="fucA"
                     /locus_tag="ACHJ12_002840"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4990602.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6860 a         7518 c         6737 g         7068 t
ORIGIN      
        1 tactaatgtt cactttcgta tgttcctttg ttgcagccca accaactatt gtcccacaat
       61 tacagcaaca agttacggat cttacgagta gcttaaactc acaagaaaag aaagaactga
      121 cgcacaagtt agaatctatt ttcaataata cccaagtgca aattgctgta ttaattgttc
      181 ccacaaccaa agacgagaca attgaacaat atgctacaag agtttttgac aattggcgtt
      241 taggagatgc caaacgtaat gatgggatac tgatcattgt tgcctggtca gatcgtactg
      301 tccgcatcca ggtaggttat gggctggaag aaaaggtaac cgatgctctg gctggagata
      361 tcatccgtag caacatgata cccgcattta aacaacaaaa atttgctcag ggattagagc
      421 tagctataaa cgctttgaat agccaactca cttcacaaca ccaatatccg actaatcctt
      481 cagaaagtga atcagcgtct tccagtgatc attattactt tgctattttt tgggtatttg
      541 cagtgatgtt cttccctttc tggttttttc atcaaggcag taatttttgt cgcgcatgta
      601 aaagtggagt ctgtatttca gcaatctatc ttttagattt attcctgttc tccgataaaa
      661 ttttttccat tgctgtattt tccttctttt ttactttcac catatttatg gtctttacct
      721 gtttatgtgt acttcagaaa agagcatcag gtaaaagtta tcattcagac aacagcggtt
      781 ccggtggagg ctcaagtggc ggcggattta gcggcggggg cggttcttct ggaggtggcg
      841 gcgcatccgg ccgctggtaa ctcaaccata aaaaatgcca gcccggaggc tggcattttt
      901 aaatcagata aagtcagtct tatgcctggc ctttgatctc tttacgaccg ttgtacggtg
      961 ctttttcgcc cagagcttct tcgatacgaa tcagctggtt gtatttagca acacggtcag
     1021 aacggctcat agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga
     1081 tggtagcgtc ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcatctt
     1141 tcgccatctt gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca
     1201 ggatggagtt agcgatacct ttttcgatac cttctttcag gatcttggtg ttagttacga
     1261 acaggtcgtc accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac
     1321 cgtcccagtc agattcgtcc agaccgtctt cgatagatac gatcggatac tgtttggtca
     1381 gttcttccag gaagtgagtg aattcttcag aggtgaaagc tttgttgcct tcgccagcca
     1441 gaacgtattt accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt
     1501 ctttgcccag ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag
     1561 cgttggaacc caggttcggc gcatagccac cttcgtcacc aacagcagtg ttcatgcctt
     1621 tcgctttcag aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt
     1681 tcacagtttt cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag
     1741 cgtgctcacc accgttgatg atgttcatca tcggaaccgg catagagtat ttgcccggag
     1801 taccgttcag ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt
     1861 tggcgttagc cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg
     1921 tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa
     1981 tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca
     2041 ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac
     2101 ctgacggagc agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag
     2161 tcgggttacc acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta
     2221 ggttttcctc aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta
     2281 cgcgttgccg ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg
     2341 ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat
     2401 ggaactggca agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat
     2461 catccccgga acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt
     2521 tgttgacttc gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc
     2581 gaaccaggct atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga
     2641 gatcgctctt ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca
     2701 gcgccacaac cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt
     2761 tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat
     2821 aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat
     2881 agccgaaacc gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg
     2941 tttcaacatc ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac
     3001 cgtgtttcag tgcttcgatc actgatttat aagcatccgg cagttcaatg tacttgccga
     3061 ccataccgat ggtgacttca cttaccgggt tcgcttcttc gaagataacc tgttcccatt
     3121 cggacagatt cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc
     3181 cctgagattt caataggccc ggaattttat agatggaatc gacgtctttc agagaaataa
     3241 ccgctttttc cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacag
     3301 cgcgatctga acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta
     3361 cagagtgctg agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg
     3421 tcaggtgcat aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt
     3481 cgaggaacgg caaggattcg atatcaccta ctgtaccgcc gatttctacc agtactacgt
     3541 catgaccttc gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa
     3601 cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga
     3661 tacgacccgt ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt
     3721 gccccaggtc caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga
     3781 ttgggctcat agtacctgga tcgacgttga tgtaaggatc cagtttcatg atggtcacat
     3841 tgaggccacg ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag
     3901 aggatacgac cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt
     3961 aggttgaaaa gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt
     4021 gccacaaact ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa
     4081 aatagcccct ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg
     4141 acacaaatgg cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg
     4201 ttgccagact tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc
     4261 aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt tcgttcgctt tttgcaatgc
     4321 gatttctgct ttcgtcccta aatggcgagc cagattaacc gtggcaaaca gcaggtcccc
     4381 catttcctcc tccagtttag cctggtcgac aacagcctgc cgcgcctcgt acatcacctc
     4441 gtcgatctct tcgtagactt tatcaaccac cgggccaagc gtcgtccagt cgaagccaac
     4501 gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa gccggtaaac tacgaggaat
     4561 atcgtccagc gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca
     4621 acgggcaagc acttcactac tgttttcggc agaactatca gcaaaaacat gcggatggcg
     4681 acgctctaat ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc
     4741 ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga tcgcccagtt caccgcgaag
     4801 atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta
     4861 aggcgcaatg gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccggatcgcg
     4921 caggcgctgc ataatagtga gcaaacggtc gatttgattc attgaattgt cctgaaaatt
     4981 gcgggtctgt caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa
     5041 tcagtacgtt aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct
     5101 tcgttgctcc tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat
     5161 cacgttcctg accggataaa acaacttcga acggatatcc ttttgattgc gttgtataag
     5221 gaacacacag acacatacct gttttgttgt tgtacatgaa aggactcagg acaacagctg
     5281 gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc
     5341 ccatatcggg tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca
     5401 gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa
     5461 tacgggctct ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac
     5521 ttcatcatca atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg
     5581 tgaatttccc caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca
     5641 tatgtagata cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc
     5701 ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcgc
     5761 cccagcactt gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg
     5821 gtgtcgctac ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg
     5881 atatcacgta acaacccact acgatcatta gccaccacgc ggaccaccag cgaatatccg
     5941 gcggagtagc tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt
     6001 tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac cgcgcccctg ggtaatgaag
     6061 ccgacaatct catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggtta
     6121 ccgacacctt caaccaccac gcgaccgtta tctttactgc ggttttgcgg cgtgtagctt
     6181 ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat
     6241 tgcgattgca ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc
     6301 agcaactcgt cgacatcatt aaagttgtaa cgcggcagca gatgtttttc tgcttctttc
     6361 aggctgatcc ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt
     6421 ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg
     6481 acgtaaccga ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata
     6541 atttcaatct ggtcgcccat ctgcagctgg taggtgaacg gcacaatgcg cccgccaatt
     6601 tttgccccga tgcagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc
     6661 gttgatcccg caggcaaatc aacgacatca cctttcggcg taaagacata cacccggtcg
     6721 tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc
     6781 cacgcaatca gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca
     6841 gctgccgcgc cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc
     6901 atctgtttgg tgcggatttg gatctcaacg gttttgccac ctggccccag aaccacggta
     6961 tgaatagact gataaccgtt tggtttcggg ttagcgacat aatcgtcaaa ctcatccggc
     7021 aggtggcgat agtgagtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg
     7081 gcgacaatac gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc
     7141 attttgcgcc agatgctgta gatgtgtttc ggtcgaccat acacttcagc tttaacgcct
     7201 tcagctttca tctcagcgcg cagatggccg acaaactctt cgatatagtg ttcgcggtcg
     7261 agacggcgtt catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg
     7321 aagcagtaat cttccagctc ccatttcagt tgcccaatcc caagacggtt cgccaacggc
     7381 gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt
     7441 acttcgcgca gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaagtcatcg
     7501 accatcgcca ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg
     7561 tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaaca
     7621 accgacttac cgacgctctc acgcagcaca tcttcgctaa ctacgttggc atcagccaga
     7681 gggaacagca gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc
     7741 accatctcaa caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc
     7801 agacaatacg cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga
     7861 cttgcgatcc atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc
     7921 gcaaccatcg tcctctcctt tagggaccag acctgccgaa atcggcaaat cgtaactatt
     7981 taacgcgcga gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca
     8041 tcgccagtcg cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg
     8101 ttgcaggatt acaggataca taaactatac gaataggttc cagttttata atttgctgca
     8161 taacacctgc ggcacctgct cgcgcggggt ccagcaacac tttatcgaag ccgtttttcg
     8221 cccacggctg ctttgtgaca tcttcttcaa gattttcatg ataaaacgtc acattctgta
     8281 aaccattaag acgcgcattc tgctggcctt tttccaccag cgccggaaca ccttctacac
     8341 ccaccacact ggcagcttgt gtcgccaatg gcagtgtaaa gttgcccata ccgcagaaca
     8401 gatccagtac gcgatcttca ggttgcacat ccagccattc caacgcacgc gctaccattt
     8461 tttggttcac acccgcattg acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc
     8521 cgtttgagtc ataccagggc atctcaccag agacggtttc gagtatctca ctatcggggg
     8581 cgagatacag atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatcagccg
     8641 aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt
     8701 caacatgacc aagatggcgt atagcttgta agctgcccag gcatgccctg actttgggca
     8761 gcaatgcttc aagttggggc actaaaatgg ggcattgttt aacgtcgaca atgtcactgg
     8821 agcccgcttt gcgaaagccc atctgaagtt gctgtgtttt cggtaagtag ttcaaactta
     8881 aacgcgcgcg acggcgatag ccccagggaa catcggcgat cacttcagag acttcgtgtt
     8941 tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt
     9001 gttgctgaca gccgccgcat acgccaaaat gaggacagcg tggcgtttcg cgttccgggc
     9061 tatcgcttaa ccggcgcacg actttagcgc gggcgtactg ttttttatct tcagtaacag
     9121 taacttccgc gttttcctgt ggcagtaatc caggaataaa taacgctttg ccgttatgtc
     9181 gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga aacggttatg atctgacgcg
     9241 tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac
     9301 agtgtgacct taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc
     9361 atgatgattc tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc
     9421 gtcgtgcatc gctataacga cttgcagcgt caactggaag atgccggtgc cagcattatt
     9481 gagccgcttg cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt
     9541 cagttaataa gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat
     9601 gatgaaaata accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag
     9661 ctcggcagca acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg
     9721 atcctccgca cgccgattat ttctgagagt tactcccccg acgaatcgcc cagtagcgat
     9781 gccaaaaata gtcaaaatat gttgggatat attgcgcttg agctggatct taaatcggtt
     9841 cgcttgcagc aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt
     9901 attgcgctta tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg
     9961 gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga aggatttatg
    10021 ctcggcgagc tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct
    10081 tatcacgaag agatgcagca caatatcgac caggcgacgt ccgatctgcg tgaaacgctg
    10141 gagcagatgg aaattcagaa cgttgagtta gatctggcga aaaagcgcgc ccaggaagcg
    10201 gcgcgtatta aatccgagtt tctggcaaat atgtcacacg agctgcgtac accactgaat
    10261 ggtgttattg gctttacccg cctgacgctg aaaacagaat taacaccaac gcagcgcgat
    10321 cacctgaata cgattgaacg ttcggcaaat aatttgctgg caattattaa tgatgttctc
    10381 gacttctcga aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc
    10441 agcacgctgg atgaagtcgt tactctgctg gcacattctt ctcacgataa agggttggaa
    10501 ctgacgctca atattaaaag cgacgtgcct gataacgtga tcggcgaccc actgcgatta
    10561 cagcaaatca tcactaacct ggtggggaat gcaattaaat tcaccgagaa tggcaacatt
    10621 gatattctgg tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt
    10681 cgggataccg gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga
    10741 caggctgatg ccagtatttc ccgccgtcat ggtggcaccg gtctggggct ggtgattaca
    10801 caaaaactgg ttaatgaaat gggcggcgat atttcgttcc atagccagcc gaatcgcggt
    10861 tcaactttct ggttccacat taatctcgat ctgaacccga acattattat cgaagggcca
    10921 tccacccagt gcctcgccgg taagcgcctg gcctatgtcg aaccaaactc cgcagcagcg
    10981 caatgcacgc tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc
    11041 tccgcgctgc ctcccgcgca ttacgacatg atgttgttag gcatcgcggt gaccttccgc
    11101 gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg
    11161 atgctggcac ttccttgcca tgcacaagtc aatgcagaaa aactcaagca agatggtatc
    11221 ggcgcgtgtc tgctgaaacc attaacacct acgcgcctgt tgcctgccct gacggaattt
    11281 tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg aaagcaagct ggcaatgaca
    11341 gtcatggcag ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat
    11401 atggtgcaac atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag
    11461 atgccgttcg atttgatctt aatggatatt caaatgcctg acatggatgg cattcgggcc
    11521 tgcgagctca tccaccagct cccccatcag caacaaacgc cggttatcgc ggtaacggcg
    11581 catgcaatgg ccgggcaaaa agagaagctg cttggcgcag ggatgagcga ttatctggcg
    11641 aaaccgattg aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt
    11701 atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc
    11761 ctcgactggc aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg
    11821 ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt
    11881 ggagaaaacc cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat
    11941 agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg aacaacagct acgtagcggt
    12001 actaaagaag aagatttgga gccggagctg ctggaactgt tggacgagat ggataatgta
    12061 gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc aatcggcttt
    12121 ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta
    12181 acgccttatc cggcctacaa aaccaattaa attcaatgaa ttaaaaaatg atgtagacct
    12241 gatacatagc acaacaggct attttgcgtt tagcatcagt ctcaagccgg ctccagatag
    12301 agccggtttt ggttttctgt cttaacgcac catgcacggg cgcttgttat cgaacgtcca
    12361 gccaggaatc agatactgca ttcccatcgc atcgtcacgc gcgccaagcc cgtgtttctg
    12421 atacagctca tgggctttca tcacttgatc catatcgatt tctacaccca gccccggttt
    12481 ttctggcacc tgtaccagcc cgcctttgat ctcaaacggt tctttggtca ggcgctgatt
    12541 accttcctgc cagatccagt gcgtatcaat agcagtaatt ttacccggtg cagcggcggc
    12601 aacatgggta aacatcgcca gggaaatatc gaagtggttg tttgagtgtg aaccccaggt
    12661 caggccaaat tcatggcaca tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg
    12721 cggatccgcc agcgggatat caacggattg cagggagagc gtatggccca tctgccgcca
    12781 gtcggtggcg atcatattgg ttgcggtcgg caggccagtt gcgcgacgga actctgccat
    12841 cacttcacgc ccggagaaac cttgctccgc accacacgga tcttctgcat aagccagcga
    12901 ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc
    12961 gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc acaatagact cggcctcttc
    13021 ttccccggcc agtacaccgc ctttcagttt gaaatcgttg aagccatatt tttcatacgc
    13081 cgcttccgcc aggcgcgcca ccgcatccgg cgtcatcgct tcttcatgac gcaggcgata
    13141 ccagtcgcat gagtcatccg gctggctttg atacggcagc ggtgtggctt tgcgatcacc
    13201 gacgaagaac agataaccga gcatttcgac ttcgctacgc tgctgaccat cgcccagcag
    13261 ggatgccacg tttaccccca gatgctgccc cagcagatcc agcattgccg cttctatccc
    13321 ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc caccagcatc
    13381 acgatcggca aaagtattac gcaccagcgt cagaacgttt ttgtattcac ccagcgtttt
    13441 acctaccacc agcggaatcg catcttccag cgttttacgg attttctcgc cgccgggaat
    13501 ttcccctacg ccagtgtgac cagaattatc tttgataatc acaatattac gcgtaaagaa
    13561 cggtgcgtgt gcaccactca gattcatcag catactgtca tgacccgcca ccgggatgac
    13621 ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt cctttttcag
    13681 aatcaatgac ggccgaaaac gggacgttta cggtcaaagg tccagccggg gatcaggtac
    13741 tgcatcggac ctgcgtcgtt acgcgcaccg acaggcagac gtttataggc ctcatgtgcc
    13801 ttttgtacct gttcccagtc cagttccacg cccagaccgg gcgcatcagg aacggcaatt
    13861 tttccgtttt taatctccag cggatttttg gtcaggcgac aatcgccctc ctgccaaatc
    13921 cagtgggtat cgatagcggt aggattaccc ggtgccgccg cgcccacatg ggtaaacatc
    13981 gccagagaga tatcgaaatg gttattagaa tggcagcccc aggtcagccc ccagtcgtcg
    14041 caaagctgcg ccacacggac tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga
    14101 atatctaccg cattgagcat caccgcatga cccatttcgc gccagttggt ggcgatcatg
    14161 ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg ccataacttc acgaccggag
    14221 aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa gaacatcatt cagccctttg
    14281 catagagaaa tggcttcatc aagtagccag gcaccgttgg gatcaacggt aatccgcgca
    14341 tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt cttgctcgcc aggtaacacc
    14401 ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc cgccagacgc
    14461 acaacggctt cgctgttcat cgctttctga tggcgcaact gataccactc atggttgccc
    14521 ggcgtatttt ccagataagg aagatcggtt ttggtccgat caccgatata aaacagataa
    14581 ccgaggacgg taatagtctc gcgttgcttg cctggcccca acagttcgca gaccggaaca
    14641 ttcagcgcct tacctagcag gtcaagcaag gcggcttcca gcgccgccac ggcattaacg
    14701 cgcaattcaa aagtccaggc acctttgccg aaggtatcaa aatcggctgc ctgattacct
    14761 ttatgcacct gctgaaccac tttattcagg cgcgcaactt cctggcccag aaccatcgga
    14821 atagcatcga ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc aatgccggta
    14881 tgcccggcgt tatcggtgag taccacaata ttgcgggtga aatatgcgtt atgtgcccca
    14941 ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa tgactttcat atcagtaata
    15001 acaggactgg attgtgtcgt catcttattg ccccgcgaca ggtttcagct cgatacgctt
    15061 gatatcgccc accagcacca ggtagctcag taccgcgatt aaggcatgaa caccaacata
    15121 aatcagcgcc ccattaaagg agccagtcgt gccaacgata taaccaattg cgattggcgt
    15181 gacgatgcca gaaatgttac cgaacatgtt gaacaggcca ccggaaagac cgctgatctc
    15241 ttttggcgcg gtatctgcca ttactgccca acccagcgcc ccgatgccct taccgaagaa
    15301 ggccagcgcc ataaagccga tgatcatcca ctcaacgttg acgtagttgc agaacaccat
    15361 caccatcgac agcaacatgc ccattacgat cggtgtttta cgcgcaatgt tcagcgatcc
    15421 cgtgcggcgc atcagccagt cggaaataat cccacccagc acaccgccga taaaaccgca
    15481 aaccgccgga acggaagcca caaagcccgc tttcagaatc gacatcccac gcgcctgcac
    15541 cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg gcgttgatac agtactgacc
    15601 gatataaacg ccgatcatca tccgtgaccc gagcagttgt ttgatctgcc cccacttcac
    15661 gctgaacggc actttaactt tggtgttttg ctgatccata ttgatcagcg cgccacccgc
    15721 ggcgatgtac tccagctctt tctgatttac ccccggatgt tgatttggct cgtggatgac
    15781 tttcaaccag ataaagctga tgacaatccc cagaccgccc ataaagaaga agacgtgtga
    15841 ccagcccact tcatgcgtca gccagcccat aatcggcgcg aagatcaccg ttgcgaagta
    15901 ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa accaggccgc
    15961 aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca agcccgacca ggaagcgcag
    16021 cgtaaacagg gcaacgataa tgccgaatcc actaaagata tcgacgaagc cttgcagcaa
    16081 ggtaaacatc gaccagataa agatcgacca gaagtagacg cgttttgaac caaaacgatc
    16141 cagcaaccaa ccaccaggga tctgcccgat aacataagcc catgagaagg cagagaacac
    16201 atagcccatt cccacgggat caaggccgat atctttggcc atttccgaac cagcaataga
    16261 aagcgtagcg cggtcgccgt agttgaagga tgtgacgata aacaacatca ccactatcca
    16321 gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag aactcattgt
    16381 tgcactcctg aaaattcgcg ttagccacgc tcactctgga ctgcgacatc gccaggaaat
    16441 cagaggtgac gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa tacggcgcgt
    16501 gtttatatct ggcaatagca gtataaaaag cgcgccatag cggctcaccg tgcaacaaca
    16561 caacattaat gcgttcaatg aggcccgatt ttggcattag ccctggacgg tggaatccac
    16621 ttcacggaaa tgaaaacaag aacaagaaag gaagggtaaa aacgaagaaa taaagaagag
    16681 tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa aatccgcgct atgaagcagt
    16741 ttttactggc atttgcctga aaagattcga ttcagcaccg ctaaaacgac atttaccgct
    16801 cgctgaacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    16861 tccggttccg ggttctcact ggcgtcaatc aacagtattt cgccaacccg ctgagcgctc
    16921 tgttcctgca acaaggcatc gaactgtttg ccgccattgc agaaatttac ataactgctg
    16981 tcgccgagcg caatcacgcc ataacgcaga ttcggctgta agcccagact atctttgatt
    17041 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17101 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17161 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17221 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17281 aggaacttat ctctccatcc tgacccgatg gcgcattgaa ctcaaccctt tcattttcag
    17341 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17401 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17461 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatcgccat
    17521 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17581 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    17641 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgagc
    17701 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    17761 ccagaactgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    17821 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    17881 acacaccaga agtgggtcgg tccagacgat gagcggtaaa aacgtgctgg ccaatctgat
    17941 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagctg cggtgaacca
    18001 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18061 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc agctcagcca gaataagcgc
    18121 gcgttgcgga tggtccgtcg ccagcgccat ttcataatag ggtgcaacgg caaaagcgcc
    18181 cggtaacggc tgtttgttat ctaacaaatc gtgcattcgc gggatcagca cccactgcaa
    18241 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18301 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18361 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18421 aaggatagca ggaataaaaa aagggagcac tgtattcaca gcgctcccgg ttcgtttcgc
    18481 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18541 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18601 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    18661 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    18721 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    18781 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    18841 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    18901 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    18961 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19021 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19081 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19141 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19201 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19261 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19321 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19381 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19441 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19501 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19561 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19621 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    19681 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    19741 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    19801 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcacgttga
    19861 acttgattac accagcgcaa atctgattga gtcgaagagt tttaagctct atctcaacag
    19921 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    19981 aagcacttgc gcccagggta aggttagcgt ggcgttatat cgtcttgatg aactggaagg
    20041 ccagccgata ggtcatttca atggcacctg cattgatgac caggatatca ctatcgataa
    20101 ctatgaattc actactgact atctggagaa tgccacctgt ggtgaaaaag tagtggaaga
    20161 gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20221 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20281 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20341 cctgttacgc ttctgccagc cagagaaatt aagcgtttac gcacgttata cccgtcgtgg
    20401 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20461 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20521 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20581 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    20641 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    20701 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    20761 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    20821 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    20881 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    20941 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21001 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21061 taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac cagctgggcc tgcgtgagct
    21121 gaatatctgc accggctgtg gtccgggagc gatggaagcg ccgatgaaag gtgctgcggt
    21181 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21241 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21301 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggaggtgt
    21361 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21421 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21481 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21541 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21601 tcgccgcgac actggtgacg cttacagctt taactggtca atgcgcattg caccagattt
    21661 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    21721 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    21781 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    21841 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    21901 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctccgctccc
    21961 ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca
    22021 tttgttatat gaatgtttct taccaccctc acgcgacaaa tatcatcaca gttaatatgt
    22081 catacaattt atgttgcaac gcaaacgttt ccctattttc ataaaccgta ttttatcgct
    22141 atgaaaaaga aattatcgcc atgattaact aaaagtattg attttttcag ttcaacctac
    22201 atatattgcg cgccccggaa gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa
    22261 ttctctttta cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagtagat
    22321 tattgtattt gagatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct
    22381 caaattatta cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg
    22441 tcaggcacct tcccgggctg aactggctaa aagctgaatt atttgcattc ctccaggaga
    22501 aatagatgga aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc
    22561 gcaagacaga caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc
    22621 tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc
    22681 ttgcgttccc gatgacgttt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta
    22741 aaaacccggg cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta
    22801 aactgattac cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg
    22861 caatcaccaa tacagttgaa agcttcatgt ctcaccagct gggtatgacg ccaccgccgc
    22921 gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc
    22981 agatgatcgt taaagcgatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc
    23041 tggctctgta cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca
    23101 ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg
    23161 tgttctcgtt caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt
    23221 acggcgatat ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg
    23281 tgctgaccgt aatgttcttc gtcttcagct gcgtactgag cctgactccg gcagacctgg
    23341 ctgcggctaa agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac
    23401 cggttatcgc gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg
    23461 gccactacct gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta
    23521 aaggtaagtc tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa
    23581 cgacctggat tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg
    23641 gcccaatcat cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg
    23701 caatgcgtaa gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg
    23761 caatctccgc aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg
    23821 cgcttcctcc gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa
    23881 tcggcattgg cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta
    23941 ccgacgatct gattgcccgt aatctgctta aagacgtgac ccgcgtggtg gttgacgtgt
    24001 acggctcgct ctctctgacc ggtaaaggcc accacactga tatcgccatt attatgggcc
    24061 tggcgggtaa cctgccggat accgtggata tcgattccat ccctggtttt attcaggatg
    24121 tgaatactca tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg
    24181 atcagtgcat gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta
    24241 ccgcgctggc gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct
    24301 ttatcgttga tgaagagcat tttggccagc agaatagcgc accggttgaa gttccttatc
    24361 cgtacagttc agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg
    24421 gcctgatgat gaaaaacgag ctggcgctgc acagcaaaga agagctggaa cagcacctgg
    24481 cgaacgtctg ggaagtcatg cgcggcggta ttgagcgcgg tatttctacc gaaggcgtgt
    24541 tgcctggcaa actgcgcgta ccacgccgtg ctgcggcact acgccggatg ctggtcagcc
    24601 aggataaaac caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg
    24661 cagtgaacga agagaacgcc gctggcggtc gcgtggtgac tgcgccgact aacggtgcgt
    24721 gcgggattat cccggctgtg ctggcgtact acgataagtt tatccgcgaa gtgaacgcta
    24781 actcactggc tcgttacctg ctggtagcca gtgccattgg ttctctttat aagatgaacg
    24841 cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt tggtgtggcg tgctcaatgg
    24901 cggcggctgg tctggcagaa ctgttaggtg caagcccggc gcaggtgtgc atcgcggcgg
    24961 aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc ggtcgccgga caggtccagg
    25021 tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc ggtgaacgcc gcacgtatgg
    25081 cgctacgccg taccagcgag ccgcgcgtct gcctcgataa agttatcgaa accatgtacg
    25141 aaacaggtaa agatatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcaatga
    25201 agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt tttgcgaggc ctcttcccga
    25261 tttctcatcc agccgtagcc tgttccggca tcgaatgtta cccttatcgc ctgatcttta
    25321 agggggttat cgtggctgtt catttgctta ttgtcgatgc actgaatctt attcgtcgca
    25381 ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca gcatgcgctc gatcagctca
    25441 ttatgcacag ccagccaacc cacgcggtcg ccgtttttga tgatgaaaac cgcagtagcg
    25501 gctggcgtca tcagcgttta ccagattaca aagcgggtcg accgccaatg ccggaagagt
    25561 tgcacgacga gatgcctgca ttacgcgccg cctttgagca acgcggcgtc ccgtgctggt
    25621 caaccagcgg caacgaagcc gatgacttag ccgccacgct ggcggtcaaa gtgacacagg
    25681 ccgggcatca ggcaacgatt gtttcgacag ataaaggcta ctgtcagtta ctttcaccga
    25741 cattacgtat tcgtgattac ttccagaaac gttggctgga tgcgccattt atcgataaag
    25801 aatttggcgt tcaaccgcag cagttgcccg attactgggg acttgcgggg atcagcagtt
    25861 caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc cacgcagctg ctggtcgagt
    25921 ttcagagtct ggaagggata tatgagaatc tggatgcggt tgccgaaaag tggcgcaaaa
    25981 aattagaaac ccataaagag atggcgtttc tgtgccgcga tattgcccgc ttacaaaccg
    26041 atttgcatat cgacggcaat ttacagcaat tgcggttggt acggtaacgg cgagccggat
    26101 acgccgcaaa cgtcgtatcc ggcattatca catcagcgca tttaccaggc ggtatggtaa
    26161 agctctacag tatcctcaag cgttgcttca cgcgggttgc caccggtaca aacatcatcc
    26221 agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc acgcaaatgt
    26281 ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc attacgcgcc
    26341 tcttccaggc tcataccttc cactttcacg cccataacgc gcgcgatatc gcggtacttc
    26401 tcaccggtaa agtcagcgtt atagcgcatg acatgcggta acaggatggc gttcgcaaca
    26461 ccgtgtggag tgttgtaaaa cgcgcccagt ggatgcgcca taccatgcac caaccctacc
    26521 ccaacattcg agaagcccat acccgcaaca tactgcccga gcgccatttc ttctccggca
    26581 tccttatcac cagcaaccga tcctcgcagc gccccagcaa tgatttcaat cgctttaatg
    26641 tgcagtgcat cggttagcgc ccacgcgcca cgggtaatat acccctcaat agcatgagtg
    26701 agcgcatcga cacccgtcgc agctttcagc gctggaggca taccatccat catgtcagcg
    26761 tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc aaacaaactt gcgccgtttc
    26821 tcttcgtcag tgatcacgta gttaatggtc acttctgccg cagtacctgc tgtggtagga
    26881 attgccagaa tcggtacact gggtttattg gtcggggaaa gcccttccag gctacgcaca
    26941 tcggcaaact ccgggttgtt gctgataatg ccaatcgctt tacaagtatc ctgtggagaa
    27001 ccaccaccaa tagcgatcag gtaatccgcg ccgctattct ggaatacacc gagcccttct
    27061 ttgacgacag taattgttgg gttgggcact acgccgtcgt aaatcgccca tgccagccct
    27121 gcagcatcca tcttatcggt cactttcgcc accacgccgc attgcaccag cgttttatcg
    27181 gtgacgatca gcgccttctg ataaccacgg cgtttcacct catcggttaa agccccaaca
    27241 gcaccccgac caaaccatgc cgtttcgttc agaatcattc tgttagccat catccttctc
    27301 cttgttgctt tacgaaatta ctcttcaatc cgtaacccat aggttttgaa tttctccagc
    27361 actacggcaa tctcttcatc gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc
    27421 aggtaaagtt gcgccagcac ttcaacttca tgcgccagcc ataacgcttt ttccagattc
    27481 acctcacaag cgataagccc atgatgttgt aacaaagttg ccttacgatt tttgagagcc
    27541 tgcgcaacat gttcagaaag ttcacgtgtt ccaaaggtcg cataaggcgc gcaaggaata
    27601 gaattaccgc cagccgccgc aatcatgtag tgaatagcgg ggatcgatcg gttaagaatg
    27661 gaaactgccg tgcaatgaac ggcatgattg tgaacaaccg cgttggcatc cggtctgctt
    27721 tgataggccg ccatatggaa acgccattcg cttgagggga gctttccttc ctcatgttta
    27781 ccgttgccat caataaagac aatatgcgac tccgtcagtt tttcatatgg aatgcctgta
    27841 ggcgtaatca gcatcccatc ctgataacgt acactgacgt tccccgctgt cccctggttc
    27901 agtcccaggc gggtcatttc caggcaagtg tcaataatct gacgagcaag tttatttcgt
    27961 tccattagct acctctctct gattcaaaac agggcaataa tgttgttcct ttcacactat
    28021 tgaattagcc gtttaattac ccaccatctt cttcctgatt aacaagaaag aaattcacaa
    28081 gcttatattt tgtgacctgg ttcaactaat cacagtaaat aactgcaagt tctcttttta
    28141 taaccccatt aaaaatgacc gctcttaaaa atatttatca aaa
//
LOCUS       DAZFFI010000057        28079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFFI010000057 DAZFFI010000000
VERSION     DAZFFI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.057"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..247
                     /locus_tag="ACHJ12_002841"
     CDS             <1..247
                     /locus_tag="ACHJ12_002841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990603.1"
                     /translation="RECRSAAFHQDFRKIRNHASEFTDLIILILIYFEKGIGIYAVFL
                     KILIIGMDFRARKGFYMDTLLIISSSHHQQFTSLFRV"
     gene            340..2763
                     /gene="pgaA"
                     /locus_tag="ACHJ12_002842"
     CDS             340..2763
                     /gene="pgaA"
                     /locus_tag="ACHJ12_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO4990604.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2772..4800
                     /gene="pgaB"
                     /locus_tag="ACHJ12_002843"
                     /pseudo
     CDS             2772..4800
                     /gene="pgaB"
                     /locus_tag="ACHJ12_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            4793..6118
                     /gene="pgaC"
                     /locus_tag="ACHJ12_002844"
     CDS             4793..6118
                     /gene="pgaC"
                     /locus_tag="ACHJ12_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO4990605.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHTALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6120..6533
                     /gene="pgaD"
                     /locus_tag="ACHJ12_002845"
     CDS             6120..6533
                     /gene="pgaD"
                     /locus_tag="ACHJ12_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO4990606.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6583..7371)
                     /gene="phoH"
                     /locus_tag="ACHJ12_002846"
     CDS             complement(6583..7371)
                     /gene="phoH"
                     /locus_tag="ACHJ12_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4990607.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7990..9261)
                     /gene="efeB"
                     /locus_tag="ACHJ12_002847"
     CDS             complement(7990..9261)
                     /gene="efeB"
                     /locus_tag="ACHJ12_002847"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4990608.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9267..10394)
                     /gene="efeO"
                     /locus_tag="ACHJ12_002848"
     CDS             complement(9267..10394)
                     /gene="efeO"
                     /locus_tag="ACHJ12_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4990609.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10452..11282)
                     /gene="efeU"
                     /locus_tag="ACHJ12_002849"
     CDS             complement(10452..11282)
                     /gene="efeU"
                     /locus_tag="ACHJ12_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4990610.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11824..13332)
                     /gene="putP"
                     /locus_tag="ACHJ12_002850"
     CDS             complement(11824..13332)
                     /gene="putP"
                     /locus_tag="ACHJ12_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4990611.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13491..13700)
                     /locus_tag="ACHJ12_002851"
     CDS             complement(13491..13700)
                     /locus_tag="ACHJ12_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990612.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13755..17717
                     /gene="putA"
                     /locus_tag="ACHJ12_002852"
     CDS             13755..17717
                     /gene="putA"
                     /locus_tag="ACHJ12_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4990613.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17757..18395)
                     /gene="rutR"
                     /locus_tag="ACHJ12_002853"
     CDS             complement(17757..18395)
                     /gene="rutR"
                     /locus_tag="ACHJ12_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4990614.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSSKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18683..19774
                     /gene="rutA"
                     /locus_tag="ACHJ12_002854"
     CDS             18683..19774
                     /gene="rutA"
                     /locus_tag="ACHJ12_002854"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4990615.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVLMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19774..20466
                     /gene="rutB"
                     /locus_tag="ACHJ12_002855"
     CDS             19774..20466
                     /gene="rutB"
                     /locus_tag="ACHJ12_002855"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4990616.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            20478..20864
                     /gene="rutC"
                     /locus_tag="ACHJ12_002856"
     CDS             20478..20864
                     /gene="rutC"
                     /locus_tag="ACHJ12_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4990617.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20872..21672
                     /gene="rutD"
                     /locus_tag="ACHJ12_002857"
     CDS             20872..21672
                     /gene="rutD"
                     /locus_tag="ACHJ12_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4990618.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21682..22272
                     /gene="rutE"
                     /locus_tag="ACHJ12_002858"
     CDS             21682..22272
                     /gene="rutE"
                     /locus_tag="ACHJ12_002858"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4990619.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22283..22777
                     /gene="rutF"
                     /locus_tag="ACHJ12_002859"
     CDS             22283..22777
                     /gene="rutF"
                     /locus_tag="ACHJ12_002859"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4990620.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22798..24126
                     /gene="rutG"
                     /locus_tag="ACHJ12_002860"
     CDS             22798..24126
                     /gene="rutG"
                     /locus_tag="ACHJ12_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4990621.1"
                     /translation="MAMFGFPHWQLKSTSIESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24209..24382)
                     /gene="ymdF"
                     /locus_tag="ACHJ12_002861"
     CDS             complement(24209..24382)
                     /gene="ymdF"
                     /locus_tag="ACHJ12_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4990622.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24755..25351
                     /gene="wrbA"
                     /locus_tag="ACHJ12_002862"
     CDS             24755..25351
                     /gene="wrbA"
                     /locus_tag="ACHJ12_002862"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4990623.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25372..25599
                     /gene="yccJ"
                     /locus_tag="ACHJ12_002863"
     CDS             25372..25599
                     /gene="yccJ"
                     /locus_tag="ACHJ12_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4990624.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25637..26878)
                     /gene="agp"
                     /locus_tag="ACHJ12_002864"
     CDS             complement(25637..26878)
                     /gene="agp"
                     /locus_tag="ACHJ12_002864"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4990625.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27167..>28079)
                     /locus_tag="ACHJ12_002865"
     CDS             complement(27167..>28079)
                     /locus_tag="ACHJ12_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990626.1"
                     /translation="VRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKVRLVLDTDPEQRV
                     NEVKNFLTALSDSICSLFIVNSNMASISLGNESFSTDEDLEYGYLMNTGNHYDVYLPP
                     ELFAQAYKLNNKEMNAQLDYLNRYAIY"
BASE COUNT         6920 a         7231 c         7019 g         6909 t
ORIGIN      
        1 ccgtgagtgc cgtagcgcag cctttcatca ggactttcga aaaatccgaa atcatgcatc
       61 ggaatttact gatttaatta ttttaatcct aatttatttt gaaaaaggca ttgggattta
      121 tgccgtattc ctgaagatcc tcatcattgg aatggatttt cgggcgagaa aaggatttta
      181 tatggacact ctgctcatca tttcttcttc tcatcatcaa caattcacgt ctctcttccg
      241 cgtttaataa cggattatga ggtgcaaaaa tatctttctt ttcagttacc tgtaattaga
      301 tacagagaga gattttggca atacatggag taatacagga tgtattcaag tagcagaaaa
      361 aggtgcccga aaaccaaatg ggctttgaaa cttcttactg ccgcattttt agcagcgagt
      421 cccgcggcga agagtgctgt taataacgcc tatgatgcat tgattattga agctcgcaag
      481 ggtaatactc agccagcttt gtcatggttt gcactaaaat cagcactcag caataaccaa
      541 attgctgact ggttacagat tgccttatgg gccgggcaag ataaacaggt tattaccgtt
      601 tacaaccgct accgtcatca gcaattacca gcgcgtggtt atgcagctgt cgccgtcgct
      661 tatcgtaacc tgcaacaatg gcaaaactcg cttacactgt ggcaaaaggc gctctctctg
      721 gagccgcaaa ataaggatta tcaacgggga caaattttaa ccctggcaga tgctggtcac
      781 tatgatactg cgctggttaa acttaagcag cttaactctg gagcaccgga caaagccaat
      841 ttactcgcag aagcctatat ctataaactg gcggggcgtc atcaggatga attacgggcg
      901 atgacagagt cattacctga aaatgcatct acgcaacaat atcccacaga atacgtgcag
      961 gcattacgta ataatcaact tgctgccgcg attgacgatg ccaatttaac gccagatatt
     1021 cgcgctgata ttcatgccga actggtcaga ctgtcgttta tgcctacgcg cagtgaaagt
     1081 gaacgttatg ccattgccga tcgcgccctc gcccaatacg ctgcattaga aattctgtgg
     1141 cacgataacc cagaccgtac tgcccagtac cagcgtattc aggttgatca tcttggcgcg
     1201 ttattaactc gcgatcgtta taaagacgtt atttctcact atcagcgatt aaaaaagacg
     1261 gggcaaatta ttccgccctg ggggcaatat tgggttgcat cggcttatct caaagatcat
     1321 cagccgaaaa aagcacagtc aataatgacc gagctctttt atcacaagga gaccattgcc
     1381 ccggatttat ccgatgaaga acttgcggat ctcttttaca gccacctgga gagtgaaaat
     1441 tatccgggcg cgctaactgt cacccaacat accattaata cttcgccgcc tttccttcgg
     1501 ttaatgggca cgcctacgag catcccgaat gatacctggt tacaggggca ttcgtttctc
     1561 tcaaccgtag caaaatatag taatgatctt cctcaggctg aaatgacagc cagagagctt
     1621 gcttataacg caccaggaaa tcagggactg cgcattgatt acgcgagtgt gttacaagcc
     1681 cgcggttggc ctcgtgcagc agaaaatgaa ttaaaaaaag cagaagtgat cgagccacgt
     1741 aatattaatc tggaggttga acaagcctgg acagcattaa cgttacaaga atggcagcag
     1801 gcagctgtct taacgcacga tgttgtcgaa cgtgaaccgc aagatcccgg cgttgtacga
     1861 ttaaaacgtg cggttgatgt acataatctt gcagagcttc gtatcgctgg ctcaacagga
     1921 attgatgccg aaggcccgga tagtggtaaa catgatgtcg acttaaccac catcgtttat
     1981 tcaccaccgc tgaaggataa ctggcgcggt tttgctggat tcggttatgc cgatggacaa
     2041 tttagcgaag gaaaagggat tgttcgcgac tggcttgcgg gtgttgagtg gcggtcacgt
     2101 aatatctggc tcgaggcaga gtacgctgaa cgcgttttca atcatgagca taaacccggc
     2161 gcgcgcctgt ctggctggta tgattttaat gataactggc gtattggttc gcaactggaa
     2221 cgcctctctc accgcgttcc attacgggca atgaaaaatg gtgttacagg caacagtgct
     2281 caggcttatg ttcgctggta tcaaaatgag cggcgtaagt acggtgtctc ctgggctttc
     2341 actgattttt ccgacagtaa ccagcgtcat gaagtctcac ttgagggtca ggaacgcatc
     2401 tggtcttcac catatttgat tgtcgatttc ctacccagtc tgtattacga acaaaataca
     2461 gaacacgata ccccatacta caaccctata aaaacgttcg atattgttcc ggcatttgag
     2521 gcaagccatt tgttatggcg aagctatgaa aatagctggg agcaaatatt cagcgcaggt
     2581 gttggtgcct cctggcaaaa acattatggc acggatgtcg tcacccaact cggctacggg
     2641 caacgcatta gctggaatga cgtgattgat gctggcgcaa cgctacgctg ggaaaaacga
     2701 ccttatgacg gtgacagaga acacaactta tacgttgaat tcgatatgac attcagattt
     2761 taaggataaa tatgttacgt aatggaaata aatatctcct gatgctggtg agtataatta
     2821 tgctcaccgc gtgcattagc cagtcaagaa catcatttat accgccacag gatcgcgaat
     2881 ctttactcgc cgagcaaccg tggccgcata atggttttgt agcgatttca tggcataacg
     2941 ttgaagacga agctgccgac cagcgtttta tgtcagtgcg gacatcagca ctgcgtgaac
     3001 aatttgcctg gctgcgcgag aacggttatc aaccggtcag tattgctcaa attcgtgaag
     3061 cacatcgagg aggaaaaccg ctaccggaaa aagctgtagt gctgactttt gatgacggct
     3121 accagagttt ttatacccgc gtcttcccaa ttcttcaggc cttccagtgg cctgctgtat
     3181 gggcccccgt cggcagttgg gtcgatacgc cagcggataa acaagtaaaa tttggcgatg
     3241 agttggtcga tcgagaatat tttgccacgt ggcaacaagt gcgagaagtt gcgcgttccc
     3301 ggctcgttga gctcgcttct catacatgga attctcacta cggtattcag gctaatgcca
     3361 ccggcagctt attgcctgta tatgtaaatc gtgcatattt tactgaccac gcacggtatg
     3421 aaaccgcagc agaataccgg gaaagaattc gtctggatgc tgtaaaaatg acggaatacc
     3481 tgcgtacaaa ggttgaggta aatccacacg tttttgtttg gccttatggc gaagcgaatg
     3541 gcatagcgat agaggaatta aaaaaactcg gttatgacat gttcttcacc cttgaatcag
     3601 gtttggcaaa tgcgtcgcaa ttggattcca ttccgcgggt attaatcgcc aataatccct
     3661 cattaaaaga gtttgcccag caaattatta ccgtacagga aaaatcacca caacggataa
     3721 tgcatatcga tcttgattac gtttatgacg aaaacctcca gcaaatggat cgcaatattg
     3781 atgtgctaat tcagcgggtg aaagatatgc aaatatcaac cgtgtatttg caggtatttg
     3841 caggcatttg ctgatcccga tggtgatggg ctggtcaaag aggtctggtt tccaaatcgt
     3901 ttgctaccaa tgaaagcaga tatttttagt cgggttgcct ggcaattacg tacccgctca
     3961 ggtgtaaaca tctatgcgtg gatgccggta ttaagctggg atttagatcc cacattaacg
     4021 cgagtaaaat acttaccaac aggggagaaa aaagcacaaa ttcatcctga acaatatcac
     4081 cgtctctctc ctttcgatga cagagtcaga gcacaagttg gcatgttata tgaagatctt
     4141 gccggacatg ctgcttttga tggcatattg ttccacgatg atgctttgct ttcagattat
     4201 gaagatgcca gtgcaccggc tatcacggct tatcagcaag caggctttag cgggagtctg
     4261 agcgaaattc gacaaaaccc ggagcaattt aaacagtggg cccgctttaa aagtcgtgcg
     4321 ttaactgact tcactttaga acttagtgcg cgcgtaaaag ccattcgcgg tccacatatt
     4381 aaaactgcac gaaatatttt tgcacttccg gtaatacaac ctgaaagtga agcctggttt
     4441 gcacagaatt atgctgattt cctaaaaagc tatgactgga ccgctattat ggctatgcct
     4501 tatctggaag gtgtcgcaga aaaatcggct gaccaatggt taatacaatt gaccaatcaa
     4561 attaaaaaca tccctcaggc taaagacaaa tctattttag aattacaggc acaaaactgg
     4621 cagaaaaatg gtcagcatca ggctatttct tcgcaacaac tcgctcactg gatgagccta
     4681 ttacaactga atggagtgaa aaactatggt tattatcccg acaattttct gcataaccaa
     4741 cctgaaatag accttattcg tcctgagttt tcaacagcct ggtatccgaa aaatgattaa
     4801 tcgcatcgta tcgtttttta tattatgtct ggtgttatgc atacccctat gcgtagcgta
     4861 ctttcactct ggtgaactga tgatgaggtt cgttttcttc tggccgtttt ttatgtccat
     4921 tatgtggatt gttggcggcg tctatttctg ggtctatcgt gaacgccact ggccgtgggg
     4981 agaaaacgca ccagctcccc agttgaaaga taatccgtct atctccatta tcattccctg
     5041 ttttaatgag gagaaaaacg ttgaggaaac catacacacc gctttagcac agcgttatga
     5101 gaacattgaa gttattgccg taaatgacgg ttcaacagat aaaacccgtg ccatcctgga
     5161 tcgcatggct gcacaaattc cccatttgcg ggtcattcat ctggcgcaaa accaggggaa
     5221 agccattgcg cttaaaaccg gagctgccgc ggcgaaaagt gaatatctgg tgtgcattga
     5281 tggcgatgcg ttattagacc gcgatgcggc ggcatatatt gtggaaccga tgttgtacaa
     5341 cccgcgtgtg ggtgccgtaa ccggtaatcc tcgtattcga acacgttcta ccctggtggg
     5401 taaaattcag gttggcgagt attcctcaat tattggtttg atcaagcgaa cccagcgtat
     5461 ctatggaaac gtatttaccg tttccggtgt tattgccgca tttcgtcgca gcgccctggc
     5521 agaagtgggt tactggagtg acgatatgat caccgaagat attgatatta gctggaagct
     5581 gcagttgaat cagtggacga ttttttacga gccacgggcg ctgtgctgga tattaatgcc
     5641 tgaaacgtta aaagggctgt ggaaacagcg cctgcgctgg gctcagggcg gtgcagaagt
     5701 attcctcaaa aatatgacaa ggttgtggcg caaagaaaac tttcgaatgt ggccgctgtt
     5761 ttttgaatac tgcctgacga caatatgggc cttcacctgc ctggtcggtt tcattattta
     5821 cgcagtccaa cttgccggtg taccgttaaa tattgaattg acacatatcg ctgcgacaca
     5881 tactgccgga atattattgt gtacgttatg tttactgcaa tttattgtca gcctgatgat
     5941 cgagaatcgc tatgagcata atctgacttc atcgcttttc tggattattt ggttcccggt
     6001 tattttctgg atgctgagcc tggcaacgac attggtatca tttacacgag tcatgttgat
     6061 gcctaaaaag caacgcgccc gttgggtaag tcccgatcgc gggattctga gaggttaata
     6121 tgaacaattt aattattacg acccgacaat caccagtacg tttactggtt gattatgttg
     6181 ccacaaccat cttgtggaca ttatttgcgt tgttcatatt cttattcgcc atggatctgc
     6241 tgacgggtta ttactggcaa agcgaggcca gaagccgact tcagttctat tttttgctgg
     6301 cagtggcgaa tgccgtcgtg ttaattgtct gggcgctgta caataagctg cgttttcaaa
     6361 aacagcagca tcatgcagcc taccaatata cgccgcaaga atatgcagag agcttagcaa
     6421 tacctgatga gctctatcag caactacaaa aaagccatag gatgagcgta cacttcacca
     6481 gccaggggca aataaaaatg gttgtttcag aaaaagcgct agtccgggca taaacaccca
     6541 aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt atgcatgcag
     6601 cgtacgttgg caaagtgccg aacgtacgca gtcctcttta ccgaaccgga cgatcccaac
     6661 catttcatct tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg
     6721 caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt
     6781 taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc
     6841 attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct tccctatttc
     6901 cggtcgcagg cagtactgca taaaggaagc ccctaagcgc cggaccagca cgtcgtagac
     6961 cgggcgaaaa tagggagcaa acttttctgc gatatctcca ggtaagaagc caagatcttc
     7021 atcggcttgc agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc
     7081 ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa
     7141 tatcagctgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag
     7201 gatgggcgaa gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgcccatctg
     7261 cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag
     7321 cacacgtttt gcctcacgac gagctttgat cactgctttt tgtcttccca tggagagcac
     7381 cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc
     7441 agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct
     7501 acgcacaccg tttgcggcgt cggtaacacg agtaaagaga ggaagtgaga aaaatacaga
     7561 gtgacgaagt tgctaccgga ggagaaatct ccgcaagtgg tggagcgtag cttaacaaga
     7621 cattgtccag aacaggacgt taccatccgc gatctccata gtgactgact atcactgccg
     7681 ggaacttccg ctgctactta ataattacaa cagatctcgc attcattgca acaatatatt
     7741 cacttatatt taaccataaa acaccatttc agtgacatta gtttctactg gaaagatgac
     7801 agagtgatga cagatatgaa aaaagctgtg tgctttcagc aggattcgca tcaggcatct
     7861 gcgcacgaac gccggatgcg gcgtgaacgc cttatccgcc taccgaaccc gcaccgattg
     7921 taggcataag aagacgcgca agcatcgcat caggcatctg cgcaagaacg ccttatccgt
     7981 ctataaacat taaacccgca ataacgcgct tccgagataa tcgttcgcgt ccttcactcc
     8041 cggcagcgca aaaaaataac cgccgccgat aggtttaacg tattcctcca gcgcttcgcc
     8101 attgagcctt ttttgtacag tcaggaagcc tttttccaga tcgtgttggt aacagacaaa
     8161 cagtagcccc atatccagtt gcccggagtt ggtgacgccc agtgaataac tgtagccacg
     8221 acgcagcatc aggctggact cactctccgc cgtgcgggga ttcgccagcc ggatatggct
     8281 gtccagcgca atcaccttcc cttccggatc gctggcgtaa tcgggaacat catgctcatg
     8341 ctgcattccc agcggcgcac cggtttgctt atcacgacca aaaatcgtct gctgttcttt
     8401 cagcggagtt ctgtcccaaa attccactcg aaactgaatc aagcgtactg cctgatagct
     8461 gccaccgatt gtccacgctg gctcctgctg atctgccgtt acccacacca ctttttgcat
     8521 caacttatca ttctggctat cgggattggc agtgccgtct ttgaaaccca gcaaattaat
     8581 cggcgtctct ttgcctttac tacgcgccgc gtgatcggaa ataaaccctt cccgcttcca
     8641 gcgcacgctg agcaaatctg gcgtatgttt gatgatatcg cgcagcgcat ggatcaccgt
     8701 gtcctgggtg ttggcgcaaa tttgtagcaa cacatcacca tgacataacg ccgcatccag
     8761 cgagtcgttg ggaaaacgcg tcatcttctg caactttttc ggcatctgtg gcgcaaggcc
     8821 aaagcgctca tcaaacaatg agtgacccac cgataacgtg atggtgagat tatcgggcgc
     8881 aatgtagccg ccaagaatgc cggaatcgag tggtggcagg cgcggatttg gcgtttctgg
     8941 tgccgctccg ccctgagtca gaaaagcaaa acgctgagtc aacaagcgaa acaaccgttc
     9001 aagatcgggt ttatcgctgg caagcacatc aaacgccacc agcatcattg cggcctgttg
     9061 tggcgtcagg atccctgcct gatgctcacc ataaaacggc tgtttctcat tgcgtgcatc
     9121 cggtgaaagc gtacccggcg cactttgcgt tttttgtgca tgagcgaccg gacaacttcc
     9181 cgccagcgcc agcgcaccaa tgcctttcag taaacgtcgg cgtgacggtt cattcacgcc
     9241 gtttttatct tcatactgca taatgcttaa tccaatccca gcacaccgcg aagttgcgcc
     9301 agatcttccg ccagcgcagt aatcggtcct ttcagtgcat tccggtcggc atcggtcaat
     9361 ttgtcgtagg tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt
     9421 ttaaagttgg catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc
     9481 agcagatcga caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg
     9541 tggctgtagc gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg
     9601 gctgcaccgc cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa
     9661 tcgaccacat cggtataaag ctggtcagcg tactgatcca tgcctttggt ggtgttgtcg
     9721 ccaaacaatg ctttttccag acggtggaaa ccggtgaatt tcggatcggc ggctttttgc
     9781 tcgtaatcat cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc
     9841 ggttcgatgc gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata
     9901 tcgcctgctt taatagcgtc agtaaaggct ttcgtgtcgg tcaccagctg cgtggtttcc
     9961 gccatgacat acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt
    10021 tgcgccgcat ccgccgttgc ctctcctttg acgatcaact tccctttcgg gttagtcagc
    10081 agaccgcagg tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaag
    10141 ccaggggcga tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc
    10201 agcgccttct ggctgtggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg
    10261 gtcatcggtt cgcactgctt atcggtcacg gtcaccttga cctgcggcac attagcggcg
    10321 ttagccataa aagcagaaga gaacagcgca gccacgctca actgcaatgc gttacggcgg
    10381 aagttaatgg tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg
    10441 cgtcgtattt gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat
    10501 gccaccagcg ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggcgct
    10561 tcctgatagc caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagagcacc
    10621 gcactcatat cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg
    10681 cgaatggcac cagctgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta
    10741 aaaaatgcac caagattgag gcgaataccg ccccagtaga gcaggaagcc tagcaccacg
    10801 gcagtagcaa gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat
    10861 gccgccagca ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc
    10921 atcaccagcg cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgctcc
    10981 agttgtactt tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc
    11041 accacggcga tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg
    11101 ccggtggttt cgttaatgaa gatccccaag cccaggcaca acgcagcggc aagcaacacg
    11161 ccaatccaca tcacaccaat ccatcggcct cgctgggtac gcttaagata gctggcaatc
    11221 aaactgacaa tcagcgcggc ttcaagtcct tcgcgcaaca taatgagaaa cggaacaaac
    11281 atgccaccca cccttaaacg tttttctgag tcaatctatg caaagaaaag taaatcgcag
    11341 tgatagcgat tatcattacg gtaaagaaaa atacaagcgg aatgtttata aaatgatgtt
    11401 gaaatgtaaa caaagagagg taatgccagg ggatataaac gattattcca ggtacaaaga
    11461 tgggattgtt tacaacgagc catgtgttct tgccggatgc cgcgtaaacg ccgtatccag
    11521 ccgacaaaac tgtccagact caatgcatca gagcttccat gtaaaactga taagcgggtg
    11581 tatcaagcaa tttcgtccat ccgcgattat caaatctaac tttttgcccg gaacataata
    11641 acagctcggg tgttggtggc gtcaaaatcg ctgaagcgtt ccccgaaggc cgggacagca
    11701 gggggccgcg gcgattggcc gccccctgcg cgctccctgc accattcaat ttatatggcg
    11761 aggcacatta agggagcgca acccgctgct gaagccaaac aaaaccgccg caggctaagt
    11821 cccttagctt tcctgcaacc gtgacggcgg agccgaatga tagtgcgcat cggcctcggc
    11881 aaagcgtttt tgcatcgccg ctgacggcgc tttacccagc aaactaaaca ctacaatccc
    11941 gatactgccg aagatgaagc ccggaataat ttcgtagagt cccagccagc cgaactgctt
    12001 ccagacgata accgtcagcg caccgatgat catccccgcc agcgcaccgt tgcgcgtcat
    12061 gcgtgaccac atcaccgaga acagcaccac cggaccaaac gccgcgccaa agcctgccca
    12121 cgcgtagctc actaagccca gcacgcggtt ttccgggttt gccgccagcg caatcgccac
    12181 cagcgccacc accagcacca tcacacgccc tacccacacc agctctttct ggctggcatg
    12241 tttacgcaga aacgctttgt ataaatcttc ggtaatcgca ctggagcaca ccagcagctg
    12301 gcaacttaag gttgacatta ccgccgccag aattgccgac agcagaatcc cggcaatcca
    12361 cgggttaaac agaatttgcg ccagttcgat aaacacgcgc tcagcattct ggtttaccgc
    12421 acctgctacc gccgggtgct cattaaagta agcaatacca aagaagccga cagctaccgc
    12481 ccctgccagg cagaggatca tccaggtcat actgatacga cgcgcatgga caatgctgtg
    12541 gtgagagtct gccgccataa aacgcgccag gatgtgcggc tgcccgaagt aacccagccc
    12601 ccaacccatc agtgagataa tggcgacaaa gttcagccct ttgagcatat caacgttttc
    12661 gatgctcttt tgtttgatca cttccagcga gtcaccaaag ccaccgacac tgataatgac
    12721 gataaccggc gtcaggatca gggcaaaaat catcaggctg gcctgtacag tgtcagtcca
    12781 gctcaccgcg aggaaaccgc caataaaggt gtaaaggatc gtcgccgcag ctcccgccca
    12841 cagagccgtt tcgtagctca tgccaaaggt actttcaaac agacgcgcgc ctgccacaat
    12901 gcccgaagcg caataaatgg tgaagaacag caaaatcacc agcgcggaga taatgcgcaa
    12961 aatgcggctt ttatcttcaa agcgcccggt gaaataatcc ggcaatgtta aggcgttatt
    13021 gttgtattcg gtatgcacac gcaaccgccc cgccaccagc ttccagttga tccacgcgcc
    13081 taatgtcagg ccaatggcga tccaactttc ggaaatcccg gaaagaaaaa ccgcgcccgg
    13141 caaccccatt aacagccagc cgctcatatc cgacgcaccc gccgataatg ccgtcacgaa
    13201 tggcccaagg ctacgaccgc ccagaatata gtcgtcaaag tttttcgttg atcgccaggc
    13261 gataaaccca atcaatatca tgccaaagat atagacacaa aatgtcacca acatcggtgt
    13321 gctaatagcc atctaaagtc tccaaaaaat tattatcggc aatgtcgaaa cttgccgtta
    13381 tatctgccac cggaacgggg taacagagtt tatgttttac cagggcgacc gtatcctgcc
    13441 ggaagcgctg gttattcaca atcgatttaa cacaccattt acattaaatt ttagtgctca
    13501 gcgacactat ttttcatcag gttgcactct ctcacatttt ttgcggttgc acctttcaaa
    13561 aatgttaact gccgcagaga aaaagtctga gttatttttt taatccctgt catatcgatt
    13621 tcttttatta acatttcatt catttttaag cttgctacgc atgtcacatt taacatggtt
    13681 gcacaaagtt gcaacatcat ggatatttca cgataacgtt aagttgcacc tttcagaaca
    13741 acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac gcgacgcgtg
    13801 agcgtattaa gtctgccgcg acacgtatcg atcgcacgcc acactggtta attaagcagg
    13861 cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag ctacctgcgc
    13921 tgctttctgg cgctgccaat gagagcgatg aagcaccgac tccggcagag gaaccacacc
    13981 agccattcct cgactttgcc gagcaaattt tgccccagtc ggtttcccgc gccgcgatca
    14041 cagcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa caagcccgcc
    14101 tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc gataaactgc
    14161 gtaatcaaaa aaacgccagt ggtcgcgcag gtatggttca ggggttattg caggagtttt
    14221 cgttgtcatc gcaggaaggc gtggcgctga tgtgtctggc ggaagcgttg ttgcgtattc
    14281 ccgacaaagc cacccgtgat gcgttaattc gcgacaaaat cagcaacggt aactggcaat
    14341 cacacattgg tcgtagcccg tctttgtttg ttaatgccgc cacctggggg ctgctgttta
    14401 ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg aaccgcatta
    14461 tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg cgcctgatgg
    14521 gcgagcagtt cgtcactggc gaaaccatcg cggaagcgtt agccaatgcc cgcaagctgg
    14581 aagagaaagg tttccgttac tcttacgata tgcttggcga agctgcactg actgccgcag
    14641 atgcacaagc gtatatggtt tcctatcagc aggcgattca cgccattggt aaagcgtcca
    14701 atggtcgtgg catctatgaa ggtccgggca tttcaatcaa gctgtcggcg ctgcatccgc
    14761 gttacagccg cgcccagtat gaccgggtaa tggaagagct ttacccacgt ctgaaatcac
    14821 tcaccctgct ggcgcgtcag tacgatattg gtatcaacat tgacgccgaa gaggccgatc
    14881 gtctggagat ctccctcgat ctgctggaaa aactctgttt cgagcctgag ctggcaggct
    14941 ggaacggtat cggttttgtt attcaggctt atcaaaaacg ctgcccactg gtgattgatt
    15001 acctgattga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg gtgaaaggcg
    15061 cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc tatccggttt
    15121 atacccgcaa ggtgtatacc gacgtttcat atctcgcctg tgcgaaaaag ctgctggcgg
    15181 tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg gcagcgattt
    15241 atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc ctgcatggta
    15301 tgggcgagcc actgtatgag caggtcaccg ggaaagttgc cgacggcaaa cttaaccgtc
    15361 cgtgtcgtat ttatgctccg gtcggtaccc atgaaacgct gttggcgtat ctggtgcgtc
    15421 gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac acctctttgc
    15481 cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg caacaggaag
    15541 ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac ggtcacgggc
    15601 gcgacaactc ggcagggctg gatctcgcta acgaacaccg tctggcctcg ctctcctctg
    15661 ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa caaccggtag
    15721 cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt gtgggctttg
    15781 tgcgtgaagc cacgccgcgt gaagtagaac aggcgctgga aagtgcggtt aataacgcgc
    15841 caatctggtt tgccactcct ccggctgaac gcgcggcaat tttacatcgc gctgccgtgc
    15901 taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc ggaaaaacct
    15961 tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac tacgccggac
    16021 aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg gtgtgtatca
    16081 gtccgtggaa cttcccgctg gctattttca ccgggcagat cgctgccgca ctggcggcag
    16141 gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg caagggatcg
    16201 ccattttgct ggaagcgggt gtaccccctg gcgtggtgca attgctgcca ggtcaggggg
    16261 aaaccgtggg cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg tttaccggtt
    16321 caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac gctcagggtc
    16381 gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc gattcttcag
    16441 cactgaccga acaagtagta atagatgtgc tggcctcggc gttcgacagt gcgggtcagc
    16501 gttgttcggc actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac acgttgaaaa
    16561 tgctgcgcgg cgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg accaccgata
    16621 tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt cagaccatgc
    16681 gtagcaaagg ccgtccagtg ttccaggcgg tgcgggaaaa cagtgaagat gcccgtgaat
    16741 ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt gccgaattgc
    16801 aaaaagaggt ctttggtccg gtgctgcatg tggtgcgtta taaccgtaat cagctgccgg
    16861 agctgatcga gcagattaac gcttccggct atggtctgac gcttggcgtc catacacgta
    16921 ttgatgaaac catcgcccag gtcactggct cggcccatgt cggtaacctg tacgttaacc
    16981 gtaatatggt gggcgcagtg gttggtgtgc agccgttcgg cggcgaaggg ttgtccggta
    17041 ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat cgcccggaaa
    17101 gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat gcacagttga
    17161 aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat cgtccagaat
    17221 tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa cgattgctgc
    17281 cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc gtgttgtgta
    17341 ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg gtgggcagcc
    17401 aggtactgtg gccggatgac gcgctgcatc gtcagctagt gaaggcattg ccatcggcag
    17461 tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccg tttgatgcgg
    17521 tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt gccgcgcggg
    17581 atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc cttctggaac
    17641 ggctgtatat cgagcgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagct
    17701 taatgacgat cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa
    17761 cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat
    17821 acctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa
    17881 tgttgagtgg aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg
    17941 ctgcttttga cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg
    18001 cccgtcagtt catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc
    18061 gaagcctgcg gataatcgcg tgagacttcc agcttcagac ggatgtactc tttgatcgcc
    18121 gccagcgggg cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc
    18181 tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag cagattggtt
    18241 tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt
    18301 gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgtacgcgaa
    18361 cgtttacccg ttgttttgac tgcgccttgc gtcatgcgct ctcccctctt gtagattggc
    18421 aacaggatag caaaaccccc gtccggcctc gaccttctgc actctcatcg cgcacggatg
    18481 catgttttat gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata
    18541 acttagcatt aacacattaa taacattcac tttttaaaaa ctggcatcca ctttgcaaac
    18601 aagccattac gcggctgaaa aggatatgca ggacgcagtg ccccgtctga ctttcacttt
    18661 acgagacgaa gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg
    18721 ctggctcatt tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat
    18781 tgtgcaaaaa gcggagcact accatttcga tttcgccctg tcgatgatca aactgcgtgg
    18841 ctttggcggc aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg
    18901 gctggcagcc gtcacctcac gcattcagat ttacgcgaca gctgccacct taacgttacc
    18961 tccggcaatc gtcgcccgta tggccgcaac cattgactcc atctctggcg ggcgttttgg
    19021 cgtcaacctc gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg
    19081 cgatgactat ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga
    19141 cctgtggggc tcggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg
    19201 cgtcagtccg caaccgagtg tactcatgaa agtgatctgc gccgggcaaa gcgacgctgg
    19261 catggcgttc tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac
    19321 acccacggct ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc aaaccgggcg
    19381 cgacgttggc tcttatgtat tgtttatggt gattgccgat gaaaccgacg atgccgctcg
    19441 cgccaaatgg gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga
    19501 acaaagtcag aaagataccc gctccggtac tgacaccaac gtccgtcaga tggccgatcc
    19561 cacttcggcg gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg
    19621 catgttagat gaagtcgcaa gcgtgcctgg tgccgaaggc gtgctgttga ccttcgatga
    19681 ttttctgtcg ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc
    19741 ccatctccct gcgctgactc aggaggtggc atgatgacga ccttaaccgc tcgaccggaa
    19801 gccattacct tcgatccgca gcaaagtgcg ctgatcgtgg tggatatgca aaacgcttat
    19861 gccacgccag gaggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc
    19921 attgccaaca ttcaaaccgc cgtgaccgca gcgcgagcgg cagggatgct gatcatctgg
    19981 tttcaaaatg gctgggatga acagtatgtc gaagctggcg gccccggctc accgaatttt
    20041 cataaatcga acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg
    20101 gcgaaaggct cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt
    20161 gtgctgccga agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttgcgc
    20221 agccgtggaa tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg
    20281 acgctacgcg atggcttttt tctggagtat ttcggcgtgg tgctggaaga cgcaacacac
    20341 caggcggggc cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc
    20401 tgggtcagcg acgtcgaaac attctgcgac gcgctttctc ccacgtcctt tgctcatatc
    20461 gcttaaggag tttaacgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc
    20521 tggctccctt cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg
    20581 cttttgatca acataataac gtgctgtttg cagatgaccc aaaggcgcaa acccgccacg
    20641 ttctggaaac catccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct
    20701 tcaacagcat ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg
    20761 agttttttcc gggtgataaa ccggcgcgat tctgcattca gtgcggactg gtaaaacctg
    20821 acgcgctggt ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt
    20881 tcactctcac ctccccctta tgctgatgcg cccgtagtgg tgttgatttc gggtcttggg
    20941 ggtagcggca gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc
    21001 tgttacgacc agcgcggcac cggcaataat cccgacacgc tggcagaaga ttacagtatc
    21061 gcccagatgg cagcggaact gcatcaggcg ctggtagccg cagggattga gcattacgca
    21121 gtggtcggcc atgcgctcgg tgcgctggtg ggaatgcagc tggcgctgga ttatcccgcg
    21181 tcggtaactg tgctgatcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc
    21241 tgttttcagg ttcgcgaacg attactgtat agcggcggcg cgcaggcatg ggtggaagcg
    21301 cagccgttgt tcctctatcc cgccgactgg atggcggccc gcgcacctcg ccttgaggca
    21361 gaagacgcgc tggcactggc gcattttcag ggcaaaaata atttactgcg tcgacttaac
    21421 gccctcaaac gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc
    21481 atctgcgcca gtgatgatct gctggtgcca tcagcatgtt ccagtgagct tcatgccgcc
    21541 ctgcccgata gccagaaaat ggtgatgcgc tatggcggac acgcctgcaa cgtgaccgac
    21601 cccgaaacgt ttaatgctct tttactcaac gggcttgcca gcctgttaca tcaccgcgaa
    21661 gccgccctgt aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg
    21721 ttcaccgatg cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta
    21781 cgggagcttt atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg
    21841 atcgtgttta tccgcactgt agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc
    21901 aatctacaaa aaaccctgac cgcgcccgtc accgctatcg ttgcctggga cagtgaattt
    21961 tatgaacggt taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc
    22021 ccacaacttg ccgaagaaac agcgtttcgc aacagctcca tgcaggcggc ctatctgatc
    22081 gtcgcttgcc gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaatac
    22141 gttgacgacg ctttttttgc aggcagcacg ctgaagagca atctgctgat taatatcggc
    22201 tatggcgata gcagcaaact ttatgcgcgc ctgccacgtc tgtcctttga agaagcctgc
    22261 gggctgttgt aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat
    22321 gtcctgtatg ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg
    22381 gttcaccgcc agcgcggtct gcagcgtgac cgatacgccg cccacattac tggtgtgcct
    22441 gaatcgtggg gcgtccgtct ggccggtatt caatgaaaat cgaacgctat gtgtaaatac
    22501 acttagcgcc gggcaggagc cgctttcaaa cctttttggc ggcaaaacgc ccatggaaca
    22561 ccgctttgcc gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc
    22621 actggtttcg tttgactgcc gtatcagcca agtggtgagc gtcggcaccc acgacatttt
    22681 gttttgcgcc atcgaagcga ttcatcgtca cgccacaccc tacgggctgg tgtggttcga
    22741 tcgcagttat cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg
    22801 gcaatgttcg gttttcctca ctggcagtta aaatcgacct ctatagagag cggcgtggtc
    22861 gcgccggatg aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg
    22921 gcgatgtttg gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc
    22981 attttaatgt cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc
    23041 agttatctcg gttccagcgc ggcttttgtc ggcgtggtga ttgccgcaac gggctttaac
    23101 ggtcagggca ttaacccgaa tatcagcatt gcgctgggcg ggattatcgc ctgtgggctg
    23161 gtttataccg tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta
    23221 atgccgccgg tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc
    23281 gcagtgaaaa gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg
    23341 tgtattgggc tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta
    23401 ggattaattg tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa
    23461 gccgtcgatt ttactctcgt cagccatgct gcctggttcg gtctgccaca tttttccact
    23521 cctgctttta atggtcaggc aatgatgctg attgcgcccg tggcggtgat tctggtggcg
    23581 gaaaacttag gtcatctcaa ggcagtcgcc gggatgaccg gacgcaatat ggacccgtat
    23641 atggggcggg cattcgtagg cgatggtctg gcaacgatgc tttccggctc tgtcggcggt
    23701 agcggcgtga ccacctatgc tgaaaatatc ggtgtgatgg ccgtgaccaa agtctactcg
    23761 acgctggtgt ttgttgctgc tgccgtcatc gccatgctgt tgggcttttc gccgaaattt
    23821 ggcgcgctga tccataccat tcctgcaccg gtgattggtg gtgcttcaat tgttgtgttc
    23881 gggctgattg ccgtcgcagg cgcaaggatc tgggtacaaa accgtgtcga tttaagccag
    23941 aacggtaatt tgattatggt cgcagtaacc ttagtgctgg gcgcgggtga ttttgctctc
    24001 acgctgggcg gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc
    24061 aatgcgttgt taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag
    24121 ccataaacag aaaaccgccg gggtaaccgg cggtgatgac tttttgacga gcttcagaca
    24181 aaaatgcatt cgtcactgat tagtccggct agttgtcact tttaccgtga ctgctcttgc
    24241 cacctttttt acctgcttca gatgcgcgct gcgggtcatt cttgaaatta cccccgctgt
    24301 gctgtccacc ttttttacct gcttctgatg ctctttcgcg gtcttctgca aaattgccgg
    24361 aaccgcctcg atggtttgcc attactgacc tccgtctggg tatagacatc atattgcata
    24421 gaattaacgc ggcgtatggc ggctgccata ttttcgctac gtgggactaa gcttagtgaa
    24481 ctgacgcggg cagacccgcg tttggtaata tcctgcaaca gcttcttagc gagaatagat
    24541 atcatctcaa ataattatcc ataagccgct cttatgatta aaaaaacgcg atgagataaa
    24601 aatgtgtcat tttgcgacaa aattacgtgc ttgtgaaagt tgttataaat caaataagtg
    24661 gttgtgaaat ttgcactctg aaaaggacgt cttatcttta aataagtggt agcgaatcgc
    24721 tacggaatag agataacacg aggagtggtt agaaatggct aaagttctgg tgctttatta
    24781 ttccatgtac ggacatattg aaacgatggc acgcgcagtc gctgagggtg caagcaaagt
    24841 cgatggcgca gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc aattatttga
    24901 aaaagcaggc ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac tggccgatta
    24961 cgacgccatt atttttggta cacctacccg ctttggcaac atgtccggtc aaatgcgtac
    25021 cttcctcgac cagacgggcg gcctgtgggc ttccggcgca ctatacggaa aactggcgag
    25081 cgtctttagt tccaccggta ctggcggcgg tcaggaacaa actattactt caacctggac
    25141 gacccttgcg catcacggca tggtaattgt ccccattggc tacgcagcgc aggaattatt
    25201 tgacgtttca caggttcgcg gcggtacgcc gtacggcgca accaccatcg caggcggtga
    25261 cggctcacgc cagccaagcc aggaagaact gtctattgct cgttatcaag gggaatatgt
    25321 cgcaggtctg gcagttaaac ttaacggcta atcttcaaca ggaggatacg catgccaact
    25381 caagaagcga aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac gtcaccggaa
    25441 atagccgagg ccattttcga agtcgccggg tacgacgaaa aaatggcgga aaagatttgg
    25501 gaagaaggta gcgatgaagt gttagtcaaa gcctttgcta agaccgataa agattcgctt
    25561 ttttggggcg aacagaccat cgaacgtaaa aacgtttaac cagcgactcc cccacttctc
    25621 gcgggggagt tttctgttat ttcaccgcct cattcaacac gctatcaaac ttatccattg
    25681 ggcagaaacc gttggcatct atcgggcaac cgcttaattc cagcgtcaca cgctgagcag
    25741 gtgcctgcag ggttaacgca tcggcattac gtaactgttc cgcactctga tacacatatt
    25801 caattttcat caaatcgcga ttggctttgc tgtcatgcca acgctggaaa acgattttgc
    25861 cgccaatcgg cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa tccagcgccg
    25921 ttaacagaga ggcaatgttg gagtcgtgcc caaccaacac tgtaattttc ggtgcgctgg
    25981 tgcgatcggt gaccagagct ttgtcgatat aactgaccag cggtttcgca acattgcgcg
    26041 ccacttccgg tgaggtaaac aggctgtcct ggtagccgtt tttaagcttc gacaacacct
    26101 tccactgctg gtcagatttg atttctcccc aggccacctg atccatcgga aaaccttcgt
    26161 aatattgcaa agtaaacgca tctaccagcg agttgccgac tttcagcggc ccggaaacac
    26221 ctggttcttg ttgatacttg gcgctaaagg tatttttgcc atccaccagc gaacactgtt
    26281 gtttctcttt acaggcaggg gaatctttat agttaacgat tttttccagt agttggtagc
    26341 tgtcggtaag ctggagtttg ctgagctctt tctccattgc cgccaccgcc tgttcactga
    26401 atgcggcgga atcatcggtg atcaccgggt taaaggttgg gtccatagtg cccatttttt
    26461 cctggtgatg cacaggaata tcacaccccg gaaatgcgcc ggtaataaag aactgtgcgg
    26521 tcgcgacagt acgttgcaga ctattggcat aggcataaac ggtgtccggc ggcgggcatt
    26581 cccccgattt taccatcccc tgctctgcca gccattcacg catgtaatgg cccatataca
    26641 cttcgagcac gccacctttg gtggtgagtt gcccaccggg gacgtcccat tctggccatt
    26701 tattcggcgt cgactgctcc agcacactgc cattgttcgc cagcggcgca cgtaagttat
    26761 ggcggctcat catgagcact tgctgtagct gatagccttc cggtacggtt tgtgcctgag
    26821 cgtttgaagc gattaaaact atccctgcca cagctgcggc gattagcgtt ttgttcattc
    26881 ctggcacctc ttttgttatc aatcactaaa gagtgtgaca gaaatatgac atttttccgg
    26941 gaacaggctc gcaaaacagc ttgttgagcg gctgttttta tattcatctt taaaaacagg
    27001 taaggtttga tgcaagctat gtctgcttat cccctgcctc gcgtacaccg ctgatgatta
    27061 cacctgtggc taaacaggat atcagcggcg ttactaatac agaggcaata gtaccaataa
    27121 taagagaagt gaggctaata acaatggttt ttagcacatg cccttgtcag taaattgcat
    27181 aacgatttaa atagtcgagt tgcgcattca tttccttatt gtttaacttg taagcctgag
    27241 caaaaagttc aggagggagg taaacgtcat aatgattgcc agtgttcatt aaataaccat
    27301 actcaagatc ttcatctgtt gaaaaggatt cattccccaa ggagattgat gccatattac
    27361 tattaacaat aaataatgag caaatgctat ctgaaagagc agtgaggaaa tttttcacct
    27421 cattaaccct ctgctctggg tcggtgtcta aaacaaggcg taccttgtta tcattaaata
    27481 taaattcggg taaatgcagt atgcattttt tgtcattcaa gcaactatca ataaattctt
    27541 ttttaaaaga caaaaaactc catgataaaa aaccaccctg cccctgaatt ttgttgtcag
    27601 attgtagatt aacgccgaat aatgtcttta catcttccct gagatcattg aatgccgtat
    27661 caacaagagc ctcgatcgtt tgtttatcaa caattttaat aaactcttcg tatacttgct
    27721 tttgcgtctt atggctgccc agcgcccatg aatactcatg ttcttttaaa accagtatgg
    27781 cccttaacag acaatagcca tcgccatcta ctggagcgta ttttaatcca taagttttat
    27841 taatttcact ctgaataaaa gatataccag acaaatcaat accagtatct aaatcagata
    27901 ttcccggcgt atttatccat tctggctgga tactcatatt ttcatcatct aaacaatact
    27961 cagaaaaaac atcatcacac gcattaatgt tttcaatata ttccataaag ccataccaat
    28021 aataaccttt aaaatctgtg atagactctg aacctctgcc cggcaaggac actctaaca
//
LOCUS       DAZFFI010000058        27284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFFI010000058 DAZFFI010000000
VERSION     DAZFFI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.058"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..741)
                     /locus_tag="ACHJ12_002866"
     CDS             complement(<1..741)
                     /locus_tag="ACHJ12_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4990627.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRR"
     gene            749..1180
                     /gene="tnpA"
                     /locus_tag="ACHJ12_002867"
     CDS             749..1180
                     /gene="tnpA"
                     /locus_tag="ACHJ12_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO4990628.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(1196..1384)
                     /locus_tag="ACHJ12_002868"
     CDS             complement(1196..1384)
                     /locus_tag="ACHJ12_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4990629.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1388..1747)
                     /gene="yeaR"
                     /locus_tag="ACHJ12_002869"
     CDS             complement(1388..1747)
                     /gene="yeaR"
                     /locus_tag="ACHJ12_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4990630.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1920..2558)
                     /gene="leuE"
                     /locus_tag="ACHJ12_002870"
     CDS             complement(1920..2558)
                     /gene="leuE"
                     /locus_tag="ACHJ12_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4990631.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2685..3608)
                     /gene="dmlR"
                     /locus_tag="ACHJ12_002871"
     CDS             complement(2685..3608)
                     /gene="dmlR"
                     /locus_tag="ACHJ12_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4990632.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3711..4796
                     /gene="dmlA"
                     /locus_tag="ACHJ12_002872"
     CDS             3711..4796
                     /gene="dmlA"
                     /locus_tag="ACHJ12_002872"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4990633.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            5047..6657
                     /gene="yeaV"
                     /locus_tag="ACHJ12_002873"
     CDS             5047..6657
                     /gene="yeaV"
                     /locus_tag="ACHJ12_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4990634.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSHGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6689..7813
                     /gene="yeaW"
                     /locus_tag="ACHJ12_002874"
     CDS             6689..7813
                     /gene="yeaW"
                     /locus_tag="ACHJ12_002874"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4990635.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7869..8834
                     /gene="yeaX"
                     /locus_tag="ACHJ12_002875"
     CDS             7869..8834
                     /gene="yeaX"
                     /locus_tag="ACHJ12_002875"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4990636.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8888..10003)
                     /gene="rnd"
                     /locus_tag="ACHJ12_002876"
     CDS             complement(8888..10003)
                     /gene="rnd"
                     /locus_tag="ACHJ12_002876"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4990637.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10085..11770)
                     /gene="fadD"
                     /locus_tag="ACHJ12_002877"
     CDS             complement(10085..11770)
                     /gene="fadD"
                     /locus_tag="ACHJ12_002877"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4990638.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11975..12556)
                     /gene="yeaY"
                     /locus_tag="ACHJ12_002878"
     CDS             complement(11975..12556)
                     /gene="yeaY"
                     /locus_tag="ACHJ12_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4990639.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12596..13291)
                     /gene="tsaB"
                     /locus_tag="ACHJ12_002879"
     CDS             complement(12596..13291)
                     /gene="tsaB"
                     /locus_tag="ACHJ12_002879"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4990640.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13349..15259)
                     /gene="yoaA"
                     /locus_tag="ACHJ12_002880"
     CDS             complement(13349..15259)
                     /gene="yoaA"
                     /locus_tag="ACHJ12_002880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4990641.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15391..15735
                     /gene="yoaB"
                     /locus_tag="ACHJ12_002881"
     CDS             15391..15735
                     /gene="yoaB"
                     /locus_tag="ACHJ12_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4990642.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            16152..16451
                     /gene="yoaC"
                     /locus_tag="ACHJ12_002882"
     CDS             16152..16451
                     /gene="yoaC"
                     /locus_tag="ACHJ12_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4990643.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(16571..16750)
                     /gene="yoaH"
                     /locus_tag="ACHJ12_002883"
     CDS             complement(16571..16750)
                     /gene="yoaH"
                     /locus_tag="ACHJ12_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4990644.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16824..18185
                     /gene="pabB"
                     /locus_tag="ACHJ12_002884"
     CDS             16824..18185
                     /gene="pabB"
                     /locus_tag="ACHJ12_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4990645.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            18189..18767
                     /gene="nudL"
                     /locus_tag="ACHJ12_002885"
     CDS             18189..18767
                     /gene="nudL"
                     /locus_tag="ACHJ12_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4990646.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18951..20315
                     /gene="sdaA"
                     /locus_tag="ACHJ12_002886"
     CDS             18951..20315
                     /gene="sdaA"
                     /locus_tag="ACHJ12_002886"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4990647.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            20446..22044
                     /gene="pdeD"
                     /locus_tag="ACHJ12_002887"
     CDS             20446..22044
                     /gene="pdeD"
                     /locus_tag="ACHJ12_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4990648.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(22048..23604)
                     /gene="yoaE"
                     /locus_tag="ACHJ12_002888"
     CDS             complement(22048..23604)
                     /gene="yoaE"
                     /locus_tag="ACHJ12_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4990649.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(23592..23750)
                     /gene="yoaL"
                     /locus_tag="ACHJ12_002889"
     CDS             complement(23592..23750)
                     /gene="yoaL"
                     /locus_tag="ACHJ12_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4990650.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            24067..25038
                     /gene="manX"
                     /locus_tag="ACHJ12_002890"
     CDS             24067..25038
                     /gene="manX"
                     /locus_tag="ACHJ12_002890"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4990651.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            25101..25901
                     /gene="manY"
                     /locus_tag="ACHJ12_002891"
     CDS             25101..25901
                     /gene="manY"
                     /locus_tag="ACHJ12_002891"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4990652.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25914..26765
                     /gene="manZ"
                     /locus_tag="ACHJ12_002892"
     CDS             25914..26765
                     /gene="manZ"
                     /locus_tag="ACHJ12_002892"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4990653.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26820..27278
                     /gene="yobD"
                     /locus_tag="ACHJ12_002893"
     CDS             26820..27278
                     /gene="yobD"
                     /locus_tag="ACHJ12_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4990654.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6632 a         7054 c         6795 g         6803 t
ORIGIN      
        1 cctgcggata tttgcgatac gggaatgcag tcgctgtatt ttgcgtttct gcttctgcca
       61 gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca gcgtcttctg
      121 gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg tggcgagttt
      181 agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag tggatacttc
      241 actttctgtc tgaatactga tgtaccactt accgcaggac tggctgaccg tgacattttt
      301 cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagcccagtt tcggcagaaa
      361 aatacggctg ttttcctgat cgagctttac gccctgcggg tagcggaatg catcattctg
      421 tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt tgtaagcccg
      481 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
      541 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
      601 attaccggcc tcatgattct cattctgacg tgccagagca cgattgaaaa cgaaacgaca
      661 tgcgcctgcg aagcgcctca tttcacactc ctgttgacca cctggtctta actggaattt
      721 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
      781 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccag
      841 ataccgactc cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa
      901 tgtatgtgct gattttgaag ctgaacttgt tgaaatggat ggcgaaccag atcacgtcca
      961 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
     1021 cgtatcgggt aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
     1081 aggcgttttg tggagtcctg gctattttgc cagtagctgc ggaggtgcgc caatatccgt
     1141 catccgccaa tacattgaac aacagcaaac acctggttag gtggaaaacc gcgccttata
     1201 tccccgccct gaaggacggg gttttacggc gcaccggata agaacgcacg gtattcacca
     1261 gatcttttat cacttcagcc gccacttctg gcaccagcaa agtcatcggc gtctctgttt
     1321 cataatcgac agaaacgcca ttgctgttat tggtgacggt cacggtatac gttgctttgc
     1381 ccatgattca tttcccgtta tgaatgactt tccgttgttg cgcaccttcc atcaggactt
     1441 caggagccac gaagaagtca atgttgaaat aagtatcgtc agtcatggct tcaatgttgt
     1501 gccacttttc tggagggaac accgcaaact gccccgcttc gataaggatc acctgatcag
     1561 gctctgcact gtgttcatca gcgtagccga gatatttgac cgccccatgc ataacggaaa
     1621 ggcgtgggta aacccccggg cgcgttcctt tatcaagatg acgttcgaat attccggcag
     1681 gcgcagtttg tttattccag aaaggcgttg agcgcgtatg aatataattc tgtgggattt
     1741 gcagcatcct attccctcct tcggggaatg cgctgaaaac ggtttattcc agccgtttca
     1801 gggtacgcct gataatttgc attttaaata ccatttattg gttacttttt agcaccatat
     1861 cagagaaaaa tcagggagga ttacaagtgg gaagcccgcg acaacgcggg ctgaatgtat
     1921 caggattgca gcgtcgccag tcgggcggcg aaacccacga acatcaaacc aatcagtgag
     1981 ttgccaactt tagccagttt ctttttggta cgtatgtact gcgtgacaaa agcaccagaa
     2041 ataatcagga agctcaaata gcagaaactc accagttcca gcgtcgtcgc cagaataaag
     2101 aatgaaattc ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa cgacacatag
     2161 aacaaaatgg ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa aatggccccg
     2221 tattggggct catcggattt ggcctcgtta tttttaccct tcagggtcgc gtagagaatt
     2281 ttactcccca gatagagcaa ataaaacgca ccaagataac gtacaatgtt gaataatatc
     2341 ggggtggtct taattaatgt cgccactcca gcccatgcca gaaacatcaa tactgcatcg
     2401 ccaataaata caccgcaggc cgcaagataa ccgcctttca taccgctact gacgctattt
     2461 ttgagtacaa acagggtatt tggccctggc accaacacaa taaaaatggc cccaaccaga
     2521 taggtccagt aattcagaac cccgtattca gcgaacacat taacctcttt aattatcttt
     2581 cgatcatgcg cgattaaagg tgaatatgct aaccaatctg tagcggctta gaaaggagaa
     2641 aatcaggttt taacccgata tcaacccgat aattgaatca ttaatcaggc atgctccagt
     2701 gaaaaattcg ggtagtgctc ggcaaaatac tggcgtaaaa attctaccgt tatccgcact
     2761 ttcgctgacg tcgccagcct tgaaacataa acggcccaga cgttcgctgg ctggtaatat
     2821 tccggtagca cttgcactaa atgaccactg gcaatgtttt cgctaacatc ccaccaggag
     2881 cgcagagcaa tcccctgccc atcgagacac cactgatgga caatttctcc atgattagac
     2941 gacagtgggc cggtgacttt aatcgcatgg tggccttctt tattgcgtaa ttgccagaca
     3001 ccaaaaggat gatcgcgctc tttaatgacc aggcacggca atgcggaaag atcggttaaa
     3061 tgtttcggcg cgccatgttg cgcaataaat tccggcgacg cgcagagaat gcggtaatta
     3121 gtcgccagtt tgcgggcgat taaattaggg gcgatgtcat cgccaatgcg aatatcgaga
     3181 tcaacacctt cattgaccaa atcgaccaac cggtcttcca catcaaaacg taattccagt
     3241 tgcggatacg ccttagccag cgctgataac gccggagcca ccacctgtcg tccaaaacca
     3301 aagctgctga taatacgcag catcccctgc ggcacctgac gcacgtcaga aagttcgtcc
     3361 atcatctgac cgacatcctg caaaatccgc tgcgcccatt cataaatccg ctctccttct
     3421 tcggtaatgg tgacgcggcg ggtggtgcgg tgtaacaaca ccacgtttag cgtttgctcc
     3481 agcaaggcga tgcgcttgct gacgaacgcc ggtgaaacgc ccagttcttc cgccacggcg
     3541 gcaaaaccag cccgacgagc caccagcata aagacgcgca aatcattcag cagcggtaaa
     3601 ttattcatga ttcgtgtttt atgtttcacc agttacgggg attaattcct tttcagtcaa
     3661 ttataggatg gtgatgttgt caattttgat ggtcaggaag tgagaaccca atgatgaaaa
     3721 cgatgcgtat tgctgcgatc ccgggagacg ggattggcaa agaagtcctt cctgaaggga
     3781 ttcgcgtgtt acaggctgcc gctgagcgct ggggcttcgc cttgagtttt gagcaaatgg
     3841 agtgggcgag ctgcgagtat tacagccatc acggtaaaat gatgccggac gactggcatg
     3901 agcaacttag ccgtttcgac gccatctatt ttggtgccgt cggctggccg gacaccgttc
     3961 ctgaccatat ttcgttgtgg ggttcgctgc tgaaatttcg tcgtgaattc gaccagtacg
     4021 tcaacctgcg cccggttcgt ctctttcctg gcgttccctg cccgctggcg ggaaaacagc
     4081 ctggcgacat cgatttttac gtggtcaggg aaaacaccga aggcgaatat tcctcgctcg
     4141 gcggtagagt gaatgaaggt acagagcatg aagtcgtcat tcaggaatcg gtatttacgc
     4201 gtcgtggtgt cgatcgcatt ttgcgttatg ccttcgaact tgcgcaaagc cgcccacgta
     4261 agacgctaac ttctgccact aaatcaaacg gtttagccat cagcatgccg tactgggatg
     4321 agcgagtgga agcaatggcc gagaattacc cggagatccg ctgggacaag cagcatattg
     4381 atattctctg cgcgcgtttt gtgatgcagc cggaacgctt cgatgtggtg gtggcgtcca
     4441 atttatttgg cgatatcctt tccgatcttg gcccggcctg caccggcacc attggcattg
     4501 ccccatccgc caacctgaat ccggaacgca ctttcccgtc gctcttcgag ccagtccacg
     4561 gttccgcgcc ggatatctac gggaaaaaca ttgctaaccc tatcgccacg atttgggccg
     4621 gtgcaatgat gctcgatttt ctcggcaatg gcgatgagcg tttccagcaa gcgcataacg
     4681 gtattctggc agcaattgaa gaagtgattg ctcacgggcc gaaaacacct gatatgaaag
     4741 gcaatgccac cacgccacag gttgccgacg cgatttgcaa gattattttg cgttaaggtc
     4801 aaaccagttt atttgaaccg cgtcactgac gcggtttttt tattcgttct ttgcagtaaa
     4861 taacctgcgt catttcacct tttattgttt ccgtttcgtg ttttatggct ttccgtattc
     4921 ttaattgttt aatttatgta acatgcaaat aatcaatttg ttaaacacaa catacaactc
     4981 attaccctgc aaataattac aactggcaaa agccagagac gggcttttat gcccaggaga
     5041 tgactcatga tgagcaatgt taagaaaaaa gatgtgccgc tgataagcat cagcctggtg
     5101 gccattcttt tcatcgcagc tgcattaagc cttttcccac aacaatcggc cgacgcggcc
     5161 aacgcaatat acacttttgt tacgcgtacg ttaggttccg ccgtacaggt attggttttg
     5221 ctggcaatgg gactggtgat ttatttagcc accagtaaat acggcaatat tcgtcttggc
     5281 gaaggaaaac cggaatacag cacgctctcc tggctgttta tgtttatttg tgccggttta
     5341 ggttcttcta cgctttattg gggggttgct gaatgggcct attattatca aacgcctgga
     5401 ttaaatatcg caccgcgttc acaacaggca ctcgaattta gcgtccctta ctccttcttc
     5461 cactggggca tcagcgcctg ggcaacttat acgctggcct cattaatcat ggcttatcac
     5521 tttcatgtgc gtaaaaacaa aggtctgagt ctttccggca ttattgccgc gattaccggc
     5581 gttcgcccgc aaggcccatg gggaaaactg gttgatttga tgttcctgat cgccactgtc
     5641 ggcgcactga ctatttccct tgttgtcacc gcagcaacct ttactcgtgg actttccgcg
     5701 ctgaccggtt tacccgataa cttcaccgtg caggcatttg tgatcctgct ttccggcggc
     5761 attttttgcc taagctcgtg gattggtatc aacaacggtt tgcaacgtct gagcaaaatg
     5821 gttggctggg gcgcgttcct gctgccatta ctggtgctga ttgtcggccc aaccgaattt
     5881 attaccaaca gcatcatcaa tgccatcggt ctgaccacgc aaaacttcct gcaaatgagc
     5941 ttattcaccg atccgcttgg cgatggttca tttacccgca actggaccgt tttctactgg
     6001 ctgtggtgga tctcatacac ccctggcgta gcaatgtttg tcacccgcgt ttcccacggt
     6061 cgtaagatta aagaagttat ctggggactg atcctcggca gcaccgtcgg ttgctggttc
     6121 ttcttcggcg taatggaaag ctatgccatt catcagttta tcaatggcgt aatcaacgtc
     6181 ccacaggtgc tggaaacact gggcggcgaa acagctgtac agcaagttct gatgtcgttg
     6241 ccagccggta aattgttcct cgccgcatac ctgggcgtga tgattatttt ccttgcctcg
     6301 catatggatg cggtggccta caccatggct gcgaccagta cgcgtaatct ccaggaaggt
     6361 gacgatcctg accgtgggct gcgtcttttc tggtgcgtgg tgatcactct gatcccgctt
     6421 tccatcttgt ttaccggtgc ttcgctggaa acgatgaaaa ccaccgtcgt gctcacagcc
     6481 cttcccttcc tcgtcatttt actggtgaaa gtcggcggat ttattcgctg gctgaaacag
     6541 gattacgccg acattccggc tcatcaagtt gaacattatc tcccgcagac accggttgaa
     6601 gccctggaaa aaacgccagt gctccctgcg ggaaccgtat tcaaaggcga caactgagcg
     6661 ccatcatcct aacgataaag gtatccctat gagcaatctg agccctgact ttgtactacc
     6721 cgaaaatttt tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt tttataccga
     6781 tcagaacgcg tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga tttgcgtcgc
     6841 tcacagcagc gaactggcga atgccaatga ttatgtgacg cgtgagatca ttggcgaaag
     6901 catcgtgctg gtacgcggtc gtgataaggt tttgcgcgcg ttctataacg tgtgtccgca
     6961 ccgtggtcat cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta cctgcccgta
     7021 tcacgcatgg gcattcaaac tcgatggcaa tctggcccat gcacgtaact gcgaaaacgt
     7081 cgccaatttc gatagcgaca aagcgcaact ggttccggtg cgtctggaag aatatgccgg
     7141 attcgtcttc atcaacatgg accccaacgc caccagcgta gaagatcagt tacccggtct
     7201 gggcgcgaaa gtgctggaag cctgcccgga agtccacgat ctgaaactgg cggcccgctt
     7261 taccacccgc acgcctgcca actggaagaa cattgtcgat aactatctcg agtgctatca
     7321 ctgtggtccg gcgcatccag gtttctccga ctccgtacag gttgatcgtt actggcacac
     7381 catgcacggt aactggacgc tgcaatacgg tttcgccaaa ccgtccgaac agtcgtttaa
     7441 atttgaagag ggtacggatg cggcattcca cggtttctgg ctgtggccgt gcacgatgct
     7501 gaacgtcacc ccgatcaaag ggatgatgac ggtcatttat gaattcccgg tggattctga
     7561 aactaccctg caaaactacg atatttactt caccaatgaa gagttaaccg acgagcaaaa
     7621 atcgctgatt gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc tggttgaaag
     7681 cgtacagaaa gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca tggccgacag
     7741 tagcggtagt ggcatttccg aacatggtat cgcccatttc cataatctgc tggcgcaggt
     7801 gtttaaggac taatgacatc ggcggcggta ttttccgccg ctgggctgat ttttgatgga
     7861 gtacagcaat gtcagactat caaatgtttg aagtacaggt gagtcaggtg gaaccgctta
     7921 ccgaacaggt gaaacgcttc acgctggtgg caaccgatgg caaaccgctg cctgcgttca
     7981 ccggaggaag tcacatcatt gtgcagatga gcgatggtga taaccagtac agcaatgcgt
     8041 attcactact gagttcgccg catgacacct cttgttatca gattgccgtt cggctggagg
     8101 aaaactcgcg cggcggttcc cgctttttgc atcagcaggt aaaagtgggc gatcggttaa
     8161 cgatttcaac gcctaataac ctgtttgcgc taattccctc agccagaaag catctgttta
     8221 tcgcgggcgg tattggtatc acccctttcc tgtcgcacat ggcagagctg caacacagcg
     8281 acgtcgactg gcagctacat tactgctcgc gaaatccaga aagttgcgca tttcgtgatg
     8341 agctagtcca gcatccgcag gctgagaaag ttcacttgca tcactcatca accggaacac
     8401 gactggaatt agcgcgatta ttggcggata tcgaacctgg cacacacgtt tatacctgtg
     8461 gtcccgaggc gctaaatgaa gcggtaagaa gtgaagctgc gcgtctggac atcgtcgccg
     8521 atacgctgca ctttgagcaa tttgctatcg aagacaaaac cggcgatgcc tttaccctgg
     8581 tgcttgcccg ttccggaaaa gagtttgtgg tgccggaaga gatgactatt ttgcaggtta
     8641 ttgaaaataa taaagccgcg aaagtggaat gtttatgtcg tgaaggggta tgcggaacct
     8701 gcgaaacagc aatactggaa ggtgaagctg accatcggga tcagtatttt agcgatgaag
     8761 agcgtgccag ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt aaacgcctgg
     8821 tgttggattt gtagtttgcc aaagccggat gtggcgctga gcgcgcccag tccggcttcg
     8881 gaagatttta ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac
     8941 cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt
     9001 tcagcagttg gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttatgcgttt
     9061 cgctcacgtc agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt
     9121 ccatcaggtt aagcatcggc tgcggtaagg cctcttccgg caatgtctgc gctttttcca
     9181 ccagcgccag cagcgtttta ccgtgaaagc ggatttcgct accggataaa cccaggctgt
     9241 ccagttcgcc taaacttccc ggcatataac gcgctaccga ccacaaatgc tcttcacgca
     9301 cgacaaagtt caccgccaga tcgcgctctc gcgccttgcg cagtcgccag tcggctaaca
     9361 gttgcagaca ggccagttgg cgtgtgcgta actgccaggc attggtgata tcacgccagg
     9421 catcttccgg cgcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg
     9481 ccgcaggtag ccagccggag gcctccgttt ctaccataag cttggcggtg atcggtaaca
     9541 gataccagac atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc
     9601 agtcggtgcg cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag
     9661 cgaaacccca tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg
     9721 gttgtggtaa ttcgccaaag acattgagga acacttccag atcttcactg cctgcatgga
     9781 gaaattttgt gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcggtgatcc
     9841 cgagaggatc aattagcgcc agatgctcgc catcgaaaag ttgaatcaac cccagctgcg
     9901 ggtaataagt gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga
     9961 cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt
    10021 tttctcttta gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta
    10081 acactcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa
    10141 tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt
    10201 agcccgtgag ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc gatggatctt
    10261 ttttcactac gaagattttc accgcttcac cactggagcc ggaaggtacg ccaacagccg
    10321 cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg ggatagacgt
    10381 taaaaccgga aaccagaatc atgtcttttt tacgatcaac aatgcgcagg aatccttctt
    10441 catccattac cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag
    10501 catcgggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacccg
    10561 gttgacctgg tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca
    10621 ccggcaaacc gatgctacca ctatgataat caatatcata tgggttaacg ctgaccagcg
    10681 gcgcacactc ggtaaggcca tagccttcca gcagatactg tccggtcagt ttcacccaac
    10741 gctctgccac cacttgctgc actggcatcc ctccgcctgc ggaaagatgc agactggaga
    10801 aatccagctg ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgcccg
    10861 tgatagcggt aaacggatat ttcgctaact cttttaccaa ccctggaata tcgcgcgggt
    10921 tagtgataag caggttctgc ccacccagtt cgataaacag caggcagtta atggtcaggg
    10981 caaaaatgtg atacagcggc agtgccgtta ctaccagttc tttacctgga tgcagcaacg
    11041 gcccataggt ggcgttgacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg
    11101 cgcctttcgc cacacctgtg gtgccgccgg tgtattgcag gaaggctaaa tcttccggca
    11161 ccagttcggg tttgacgtac tgcatccggt agccgttatg cagtgcgcta cgaaatgaaa
    11221 tggcatctgg cagatggtat ttcggcacca aacgcttgat gtatttaaca acgaaattga
    11281 ctaccgtgcc ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa
    11341 cggcggtttt atcaaccact ttttccagtg tgtgagcaaa gttagacacg ataacaatcg
    11401 ccgatgcgcc gctatcgtta agctgatgct caagctcacg cggggtatac aacgggttaa
    11461 cgtttacgac gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata
    11521 aattaggcat catcaacgca acgcgatcgc ctttcttcag ccccaaccct tgttgcaaat
    11581 aagcggcaaa cgcgcgactg cgttcttcca gcttgcggaa ggtcattacc tcccccatat
    11641 tcacaaacgc aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag
    11701 attgataacg gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa
    11761 ccttcttcaa ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata
    11821 tacatgccgt taacataata ttaactcatc ataccagctt gataattacc caacgaaaag
    11881 gttgcgaagc gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac
    11941 cagccgctgt ttctttttct ttaaaaacaa gcaattattc agttacaact gtttgtactc
    12001 tcgcggggcc gggattatac cagccccatc cgccgtagcc atagggccag ccacgaccgc
    12061 cataaaacca tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac
    12121 gtttgtaacc cgttacttgc atcaccataa atttataggg cgtgttgccg atttttccgt
    12181 caaccgcacc agtgattggc ccgactaccg taaccagttg tccacggaaa tccaccgggt
    12241 ccagaaaacc gttcacatcg gcataaatgc gaccgcgaga aggttctccc agcgtcggtc
    12301 tggcaccgct gtccagcggt acggtagcaa tttccaggcg ggttttccct tgctggtttt
    12361 gtaccgcaac cactttgcca ccaaagcgtg cctcctgacc aacgtacagc tgcggcgcac
    12421 tcatcacccg aactaaatct tgttgcggcg tgggactgct gcctttaatg gcgtccggca
    12481 cagtgacaca accgctcagc attagcgcaa acgtacctgc cagtatgcct ttgataacat
    12541 tcttttgaac cgccatgatg cgactccttt ttttctcagg gcatactctt aagattcatt
    12601 ctttgcccgg aagtttcttc catgcgacgt tgttacgtaa ataaaccggt tcggcatgtt
    12661 ccaccgccac cgttttaccc tcggcaaaca tctgacaagc aatcggcagc atatcttctg
    12721 cagcggggag taacacttcg ccatcgcgca aaaccagtcc gctctcttta ccgagatccg
    12781 gccaggcttg ccagcccgtc cctaccgtca cccattcgcc ggaaagctgt tgcattcgtt
    12841 catggacgat ttcgggtttg agtacggctt cggtttcttc accgtgccag ataccgtttt
    12901 catcacgctg atattcggcc cagtaaactt cgcccattcg cgcgtcaatg gctgccagca
    12961 cacgggttgc gccgtttttg cgccacgccc cttgcgccat cgtcattagc gtggagacgc
    13021 caatcatcgg taattccgcg ccaagcgcca gcccttgcgc gatgccaata ccaatgcgca
    13081 cgccagtaaa gctaccgggg ccgcgcccgt aagccagagc gttaatatca gtcagggaag
    13141 ttccgctggt ggtcaggata tcctgcacca tcggtaagat tcgttgagta tgttcacgag
    13201 ggcaaagctc aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca gagcaggcct
    13261 ctgtcgcggt atcgatagcc agaattcgca tgggtcttcg tgcttagatc aataaaaagg
    13321 cgcgcatcat accatactcc gtaacaaatt acctggagga tggtatcgca aggaaacgaa
    13381 ccgcacgggc aatgtcacgg gtgcgtggcg cgggcggcag actggcgaga aacgtcgcgc
    13441 cgtaagggcg catcaccagc cgattgtcac aaatcaccag cacgccacga tcgtcggcgt
    13501 cgcgaatcag tcgccctacc ccttgtttga gagtaatgac ggcatctggt agttgcactt
    13561 catcgaacgg gtcgccaccg cgcaaacggc aatcttccat gcgcgctttt aacagcgggt
    13621 catccggtga ggtaaacggc aacttgtcga taatcaccaa tgacaatgta tcgccacgca
    13681 cgtccacccc ttcccagaaa ctactggttg ccacaagaag cgcgttaccg gcgctgacaa
    13741 attgctgcaa cagttgccct ttgctggttt ccccctgcaa caaaacggga agcgtcatgg
    13801 tagcgcggaa ctgctcggcc agatcgcgca tcatggcgtg cgaggtacaa agcataaaac
    13861 aacgaccgtt gttagcttcg atgatcggtc gcagcattgc cgctaactgg cgagcagaac
    13921 ctggctggtt ggtttgcggc agattgcgcg gcacacagag taacgcctgg cggctgtaat
    13981 caaacgggct tggcaatagc aacgactccg cctgttctat cccaagccgc gaggtgaaat
    14041 gatgcagatc gtcgttcacc gacagcgttg ctgaggtgaa gatccagcta ccgggctttt
    14101 gcgccattaa ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc agagtaaaat
    14161 ggcgcgaagt gcattcgtac cagtagctgt agcccggctg attgatctct tttagccgct
    14221 tcagccgtgt gcgatacaac gtggcgcgct caaatgccgc atccagcaag gcggaacgcc
    14281 ccagtgacag tttcgccacg tcataacaaa gttccagggt gtcatcgagc agtaaaaatg
    14341 cccgctgaat ttgcggatta gctaacagct cacgcaggtt accgcgataa cctggctcac
    14401 cgagttgcag acgaaaatcc tgcgcactct gggcaagacg atcagcgcac ttttgtaact
    14461 gctgggtgtc ttttaattcg gtgcggtagg cgatggtgat gtcttttgcc aggtcgagca
    14521 gttgtcgact ggagagtgac tgaccaaaat actggctggc aatgtccggt agctggtggg
    14581 cttcgtcgaa gatcatgacg tccgcttccg ggatcaactc gccaaatcca ctctctttaa
    14641 ccaccatatc cgccagaaag agatgatggt ttaccaccac cacatcggcg tccatcgctt
    14701 ttttacgtgc tttgaccaca aagcaatctt tatacatcgg gcagtcgctg ccaagacagt
    14761 tgtcgttggt gctggtgacc agcggccacg cctgtgaatc ttccgccacg ctgacgcagg
    14821 tgctgatatc accatcgact gtttgattag accaggagcg cagcaggatc acatcgctta
    14881 agatttgtac cggcagatcg ccccccgcca gcgcctgctg ttcgagacgt tcaaggcaga
    14941 ggtagtttga gcgccctttc agcagcgcca cgttgcccgt gtatttcaac gcctttgaga
    15001 ctgttggcaa atcgcggctg tagagctgat cctgcaacgc ttttgagccg gtcgagataa
    15061 tgactttctt tttcgcccgc agcgcaggag ccaggtaagc gtaggttttg cccgtaccgg
    15121 ttcctgcttc caccaccagc ggctggcctt tttctatcgc ctgggtgacg gctaccgcca
    15181 tctgtcgctg tggttctcgc ggcttaaagc ctggtatcgc tttcgccagc tgaccgtctg
    15241 gtgcaaaatc gtccgtcaca ctaccccctg ttgatttgaa cagggattat gtcaggatga
    15301 gggcgctttc gccagttgaa gtggtgacgg cgacctcaca ttgtggcagt ctttgcagca
    15361 cgaaaatgga aggataatga ggacaaaatg atgactatcg ttcgtatcga tgctgaagcc
    15421 cgctggtctg atgtggtaat ccacaacaac acgctctact acactggtgt accggaaaac
    15481 ctcgacgccg atgcctttga gcaaaccgcc aacacgctgg cgcagattga tgccgtgctg
    15541 gaaaaacagg gcagcaataa atcgagcatt ctggatgcca ccattttcct ggccgataaa
    15601 aacgacttcg cggcgatgaa taaagcgtgg gatgcctggg ttgtcgcggg tcatgcgccg
    15661 gtgcgctgca cggtacaagc gggtttgatg aacccgaagt ataaagttga aattaagatt
    15721 gtggctgcgg tgtaagcttt atcgaagcaa aataagtcag acgataattt atcgataata
    15781 ctggtcggtt ttacataaat cgaccagaga ttaagatatt acctgttgat gctcctgcta
    15841 attcattatt tatttttata atttcaattt tatctataaa actaactata taaataacca
    15901 tgccgttgtg ttcgccctct gactagcaac tctggttttc tttcttagct gaataatctg
    15961 gttatagcgt gaggactgac cgttaaattt ctccatctta ctggataaca caccatgagc
    16021 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc
    16081 tttgatatta gatgcaaatt aaggtcatat atagtcttat cgataacatg gttaatttta
    16141 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
    16201 tatcagcgcc aacaccaagt tcgatgaatg aaagcacagc gaagggaatc tttaaatatt
    16261 taaaagaact gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag
    16321 gctggaaccc tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg
    16381 aacgcactgt aattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
    16441 cactggtctg agcttaattt atatcagcat aaatgggtca ggacgctttt aatcacatat
    16501 aaaaagcgtc ctttttctca caatcaaata atcatcggat tctataacga aatcgatacg
    16561 gattagtgtg ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt
    16621 tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca
    16681 tcagttcctg gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac
    16741 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
    16801 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
    16861 ggcgtcagga cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc
    16921 ttttacactc cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc
    16981 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca
    17041 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
    17101 gcccaacgca taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg
    17161 atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg
    17221 atatggcagt gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt
    17281 ctttgctgag tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct
    17341 cgccgcagga agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt
    17401 acggcgaaaa atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga
    17461 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
    17521 ttaatcaggc caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt
    17581 taagcctttc gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccga
    17641 ttaaaggcac gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac
    17701 tggcgaactc agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
    17761 atatcggtcg tgttgccgta gccggttcgg taaaagtacc agagctgttc gtggtggaac
    17821 ccttccctgc cgtgcatcat ctggtcagca ctataacggc gcgactacca gaacagttac
    17881 acgccagcga tctgctgcgc gcagcttttc ctggtggctc aataaccggg gctccgaaag
    17941 tacgggctat ggaaattatc gacgaactgg aaccgcagcg acgtaatgcc tggtgcggca
    18001 gcattggcta tttgagcttt tgcggcaaca tggataccag cattactatc cgcacgctga
    18061 ctgccattaa cggacaaata tactgctctg cgggcggtgg aattgtcgcc gatagccagg
    18121 aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc caactggaga
    18181 agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatctcgc tttcagcttt
    18241 tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca
    18301 tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac
    18361 acgctggaca agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg
    18421 ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaagtta
    18481 tcggcgtgct gccgcccgtc gatagcgtaa ctggctatca ggtaacccca gtggtcggca
    18541 ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggcg gtgtttgaaa
    18601 tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg
    18661 gcgattcaca tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg
    18721 caggcataat tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt
    18781 tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg
    18841 attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc
    18901 tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc
    18961 tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga
    19021 aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc
    19081 gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata
    19141 tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc
    19201 ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacggcatg
    19261 aagtggattt cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg
    19321 aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt
    19381 attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg
    19441 aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa
    19501 ccggctattc gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag
    19561 agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga
    19621 tgaacaccga aggtgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tctgccctgc
    19681 gccggatgtt agtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg
    19741 taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg
    19801 cgccaaccaa cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta
    19861 ttgaatcggt cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg
    19921 cattgtataa aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg
    19981 gtgttgcctg ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac
    20041 aggtttgcgt ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg
    20101 ttgcagggca ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga
    20161 ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg
    20221 tcatcgaaac gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac
    20281 gcggtggtct ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac
    20341 ggatgggcga atttataccc gctttctcgt ctgctgtaat attccccact acacttccac
    20401 tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa
    20461 cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt
    20521 acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg
    20581 gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag
    20641 gcaggtagcg aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca
    20701 ttacgtaaac aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc
    20761 acactctatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct
    20821 gaacttcctg caccgcaacc acttttacgc ctgacgatcg accgtgccct gattaaaggc
    20881 agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag
    20941 atgattaaca tcgacttact gacggcaatg ctccttgagc cacaactgcc gcaaatcagt
    21001 agcgccagcc tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc
    21061 cttccgcaac ctgaagacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc
    21121 attaacgtta atggtccggg ggctacggcg ctggcatggc actatcttcc aacacaatta
    21181 ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac
    21241 cggatgagct tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc
    21301 tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc
    21361 tggaataatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa
    21421 catcatttaa ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat
    21481 gttttcccga tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc
    21541 cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa
    21601 ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc
    21661 gagctacatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca
    21721 ttttcctggc ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca
    21781 gctataggtt ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa
    21841 agactgaata ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg
    21901 cgccgtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt
    21961 gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc
    22021 acgcccatta tgccgttacg ttaatgctta ctcatcttca tcgtgcgccg gttgctcttt
    22081 aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg
    22141 ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc
    22201 cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa
    22261 ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg
    22321 cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac
    22381 cagaccttgt accacaccaa actcgttggt cacgataaca aagctcccgc gagcacgacg
    22441 cagcacgccc aacaggttga tcggatcgag ggtttccggg acgataatcg ccggagacgc
    22501 cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg
    22561 tacaataccg atgatttcat ccagttcacc gcgacatacc gggaacagac tgtgcggtga
    22621 agagagcagt tgctcgcgga tttcatcgac cccgagatta gcgtcaaccc agcttatttc
    22681 accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat
    22741 catgtaacgt tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc
    22801 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
    22861 agtacgcgct cgcagcggca aagtcgactg gtggcgaata aagttgcgac gcgcaatctg
    22921 gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
    22981 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
    23041 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggtttgg atgccagcaa
    23101 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc
    23161 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
    23221 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
    23281 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat
    23341 caggtcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
    23401 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccag
    23461 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
    23521 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
    23581 tgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
    23641 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agagccagaa
    23701 aggtaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
    23761 actgccctat actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt
    23821 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
    23881 ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
    23941 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
    24001 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
    24061 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
    24121 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
    24181 ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc
    24241 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc
    24301 cgcattgtcg tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc
    24361 gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt ggcactggca
    24421 gtagaaacag gccgtgaagg cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg
    24481 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca
    24541 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
    24601 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
    24661 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
    24721 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
    24781 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
    24841 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
    24901 aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt
    24961 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
    25021 agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt
    25081 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
    25141 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
    25201 tcgcgtgtac cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg
    25261 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg
    25321 ccgctctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
    25381 caggtatcgc actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc
    25441 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
    25501 cagcgatttc ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggcaattc
    25561 ctgccgtcat cgtggcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
    25621 ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt
    25681 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
    25741 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
    25801 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
    25861 aggcagctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    25921 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc
    25981 tgcgttctaa cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    26041 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    26101 aagctattcg ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc
    26161 tcggcgtaac cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    26221 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    26281 tctggggaac cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca
    26341 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    26401 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    26461 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    26521 tggttaacaa gtggacacat gtcaacatcc cgctggtggt ttcgaccatc accgggcagg
    26581 atggacagac ccgtgtcacc accgtgcaaa ccattcttga ccagttaatg ccaggcctgg
    26641 taccactgct gctgaccttt gcttgtatgt ggctgctgcg caaaaaagtt aacccgctgt
    26701 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    26761 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    26821 tgacaatcac ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct
    26881 acgatcagtt catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    26941 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    27001 acgtcacgaa tcatggcgcg ctaataacaa catggttatt aagtgcactg gctctgatgg
    27061 gtttttatat attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct
    27121 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    27181 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc
    27241 tggaaaaaat ttataagctt ctcgtatcaa ctcaataact tatg
//
LOCUS       DAZFFI010000059        27177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFFI010000059 DAZFFI010000000
VERSION     DAZFFI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.059"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..645
                     /locus_tag="ACHJ12_002894"
     CDS             <1..645
                     /locus_tag="ACHJ12_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990655.1"
                     /translation="YSNEYANSGVAALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSK
                     NFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLELFNA
                     SQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNII
                     SAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
     gene            complement(717..1133)
                     /locus_tag="ACHJ12_002895"
     CDS             complement(717..1133)
                     /locus_tag="ACHJ12_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990656.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(1172..2401)
                     /locus_tag="ACHJ12_002896"
     CDS             complement(1172..2401)
                     /locus_tag="ACHJ12_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4990657.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(2567..2929)
                     /gene="ssrA"
                     /locus_tag="ACHJ12_002897"
     tmRNA           complement(2567..2929)
                     /gene="ssrA"
                     /locus_tag="ACHJ12_002897"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3144..3626)
                     /gene="smpB"
                     /locus_tag="ACHJ12_002898"
     CDS             complement(3144..3626)
                     /gene="smpB"
                     /locus_tag="ACHJ12_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4990658.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            3797..4234
                     /gene="ratA"
                     /locus_tag="ACHJ12_002899"
     CDS             3797..4234
                     /gene="ratA"
                     /locus_tag="ACHJ12_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4990659.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            4224..4514
                     /gene="yfjF"
                     /locus_tag="ACHJ12_002900"
     CDS             4224..4514
                     /gene="yfjF"
                     /locus_tag="ACHJ12_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4990660.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(4576..4917)
                     /gene="bamE"
                     /locus_tag="ACHJ12_002901"
     CDS             complement(4576..4917)
                     /gene="bamE"
                     /locus_tag="ACHJ12_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4990661.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(5066..6727)
                     /gene="recN"
                     /locus_tag="ACHJ12_002902"
     CDS             complement(5066..6727)
                     /gene="recN"
                     /locus_tag="ACHJ12_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4990662.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6813..7691)
                     /gene="nadK"
                     /locus_tag="ACHJ12_002903"
     CDS             complement(6813..7691)
                     /gene="nadK"
                     /locus_tag="ACHJ12_002903"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4990663.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7814..8407
                     /gene="grpE"
                     /locus_tag="ACHJ12_002904"
     CDS             7814..8407
                     /gene="grpE"
                     /locus_tag="ACHJ12_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4990664.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(8462..9748)
                     /gene="yfjD"
                     /locus_tag="ACHJ12_002905"
     CDS             complement(8462..9748)
                     /gene="yfjD"
                     /locus_tag="ACHJ12_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4990665.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(9769..10560)
                     /gene="ypjD"
                     /locus_tag="ACHJ12_002906"
     CDS             complement(9769..10560)
                     /gene="ypjD"
                     /locus_tag="ACHJ12_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4990666.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            10727..12088
                     /gene="ffh"
                     /locus_tag="ACHJ12_002907"
     CDS             10727..12088
                     /gene="ffh"
                     /locus_tag="ACHJ12_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4990667.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            12337..12585
                     /gene="rpsP"
                     /locus_tag="ACHJ12_002908"
     CDS             12337..12585
                     /gene="rpsP"
                     /locus_tag="ACHJ12_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4990668.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            12604..13152
                     /gene="rimM"
                     /locus_tag="ACHJ12_002909"
     CDS             12604..13152
                     /gene="rimM"
                     /locus_tag="ACHJ12_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4990669.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            13183..13950
                     /gene="trmD"
                     /locus_tag="ACHJ12_002910"
     CDS             13183..13950
                     /gene="trmD"
                     /locus_tag="ACHJ12_002910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4990670.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13992..14339
                     /gene="rplS"
                     /locus_tag="ACHJ12_002911"
     CDS             13992..14339
                     /gene="rplS"
                     /locus_tag="ACHJ12_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4990671.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14416..14898)
                     /gene="yfiB"
                     /locus_tag="ACHJ12_002912"
     CDS             complement(14416..14898)
                     /gene="yfiB"
                     /locus_tag="ACHJ12_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4990672.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14914..16140)
                     /gene="dgcN"
                     /locus_tag="ACHJ12_002913"
     CDS             complement(14914..16140)
                     /gene="dgcN"
                     /locus_tag="ACHJ12_002913"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4990673.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(16130..16648)
                     /gene="yfiR"
                     /locus_tag="ACHJ12_002914"
     CDS             complement(16130..16648)
                     /gene="yfiR"
                     /locus_tag="ACHJ12_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4990674.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(16798..17163)
                     /gene="yfiL"
                     /locus_tag="ACHJ12_002915"
     CDS             complement(16798..17163)
                     /gene="yfiL"
                     /locus_tag="ACHJ12_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4990675.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            17373..18443
                     /gene="aroF"
                     /locus_tag="ACHJ12_002916"
     CDS             17373..18443
                     /gene="aroF"
                     /locus_tag="ACHJ12_002916"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4990676.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            18454..19575
                     /gene="tyrA"
                     /locus_tag="ACHJ12_002917"
     CDS             18454..19575
                     /gene="tyrA"
                     /locus_tag="ACHJ12_002917"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4990677.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(19618..20778)
                     /gene="pheA"
                     /locus_tag="ACHJ12_002918"
     CDS             complement(19618..20778)
                     /gene="pheA"
                     /locus_tag="ACHJ12_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4990678.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(20800..20925)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(20877..20924)
                     /gene="pheL"
                     /locus_tag="ACHJ12_002919"
     CDS             complement(20877..20924)
                     /gene="pheL"
                     /locus_tag="ACHJ12_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4990679.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            complement(21028..21369)
                     /gene="raiA"
                     /locus_tag="ACHJ12_002920"
     CDS             complement(21028..21369)
                     /gene="raiA"
                     /locus_tag="ACHJ12_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4990680.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(21640..22377)
                     /gene="bamD"
                     /locus_tag="ACHJ12_002921"
     CDS             complement(21640..22377)
                     /gene="bamD"
                     /locus_tag="ACHJ12_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4990681.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            22512..23492
                     /gene="rluD"
                     /locus_tag="ACHJ12_002922"
     CDS             22512..23492
                     /gene="rluD"
                     /locus_tag="ACHJ12_002922"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4990682.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRLHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            23489..24220
                     /gene="yfiH"
                     /locus_tag="ACHJ12_002923"
     CDS             23489..24220
                     /gene="yfiH"
                     /locus_tag="ACHJ12_002923"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4990683.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            24350..26923
                     /gene="clpB"
                     /locus_tag="ACHJ12_002924"
     CDS             24350..26923
                     /gene="clpB"
                     /locus_tag="ACHJ12_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4990684.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6762 a         6306 c         7050 g         7059 t
ORIGIN      
        1 tattcaaatg aatacgctaa cagcggagtt gctgcattgc tccaatattg ctcttcaaaa
       61 atggatctgc catcaaataa tgatacttat cacgaaatgt atcatgcttt aaatatgaat
      121 ttgtcttcaa agaattttga agatggtcat atttctactg gcactatttt ctttgataca
      181 gagtcgaaca aatggtattt atgtgtatct gcggcatgtg atttggttcc tactcagggt
      241 aacgaccctc accatgtaag attaagtccg cacaggctca ttaaagttct ggagcttttt
      301 aacgccagtc agagtaaagc attgccattt gctgaacatt cgaaatatat atatgtaatg
      361 cataaaaatc aaagaaaata tctctctatt ttcgaagggg ataaaacgct tcctgttgtt
      421 gattatatgg tggtgttgaa tcatggaaca acagttgatg gcgaagaaaa aaatattatt
      481 tctgccgtgt ttttaagtaa tatggatggc aacgtgcaaa atgttcctgt ccgactcaaa
      541 cttaaatctc aactgagaac tggttatgca gaaagatatc aggctatagc gtctcagtat
      601 agctcaagga ttggtgtgga ttatgtatca atgatgctac cataattatt atattttagg
      661 cgtggtgatt ttttttcgcc atgcctattt tatatttatc atcacaatga tgttgtttat
      721 ttttgtttaa gtcttctaag cttcatgcat tctaatgaga ataataatag agttgtgctg
      781 tatataaagc cagagttagt taataacttt aaatttaatg cttcaggatt ttcactgaga
      841 aagtgaaagg cataaactaa gtatacagct aaagtagccg ttccaattgt tggtccaata
      901 tcgccttgat catgaggttc aaggtttaca ttgaacttga agattagcca ttcaaatccc
      961 caagtaaaaa aaacaactaa tatcatggct gcgaataatt tcgcgatgtt atctgctgat
     1021 ggattcatca gaacagattg tttctgaaaa aattcacaaa gtgagagtcc gaaaaaaata
     1081 aaaaaaagtg gtctggaact aaatttttca aaaactttga atagggtttc cattttcttt
     1141 accttcagga ttatattagc tattctgata cttaggctac cagagcattt gatctttgag
     1201 cttgaacgta atcactccac cattgcatca aactctgtcg ctctatcaga tattctgcac
     1261 gattgtatgc tgcgataatt tcatcttttt tcgagtgggc aagcgctgcc tcaagaactt
     1321 cagctctgaa tttaccagac tcctctgccg ctgttcgtgc aatagaacgc ataccgtgag
     1381 ctacaagctc gcctccgaac cccattcgga tgatagctgc gttggctgtt tgttcatgca
     1441 tatgattaag aggcgctttt atgctgggga aaacccattc tctatgccca cttattgatt
     1501 tcattaattc aaggatgcgc aaagcttctt tactcaaagg aactttgtga agctttttca
     1561 ttttcatgaa atcagcagga atgttccaaa tgctgttggt tgtatcaata tcagaccacc
     1621 ttgcgcgaac ggcttcaccc ggacgaaccc atgtcaacaa ttgccattca attagcatac
     1681 gtgtttccaa ccggattgac gcattcgtca aagattccat aaaccttggc aattcgcttg
     1741 ggggaagggc aggcatattt tgcttttttg gtttactgaa tctttgacca aggttgtcag
     1801 ccgggttgaa ctcaataagt tcttcagtag ctgcccaccg gaagatttca ttcagacgtg
     1861 aaatgatacg gcgtagagtt tccaatacgc ctcgttgctc aataggatca aggtgttgtt
     1921 ttaagagctt aggtcggatc tcattgatag ggacattacc cagaccggga aagacatttc
     1981 tctctaagct gcgccagatg tctgctgcat ggtcttgtga gatacctgat gtctttacct
     2041 tctcatctaa ccatttccgc gctacggctt ggagagtgtg ctcagtagca ctctttaatg
     2101 ccttcgcctt atcgttgtta tggatttggg gatcaacacc atttgccaga aaggagagat
     2161 attcatcacg taaggctctg gctcttgcaa gggtaaggtg aggatatgtc ccaaggctca
     2221 ttttggttct tttcttgctc actggtactg catacctgaa ataccaattt ttcttgcctc
     2281 ctttcgccaa aggagcgatt cgtagaatca gaccatcacc gtcaaacaag ttgatttctt
     2341 tatcggctgg cttggtgctt ttgatttcag tgtcagtgag cttcttagcg atttttgcca
     2401 ttttgggacc ctcggttttt ggacccttct tagtgggtcc cattcagggt gccataactc
     2461 gtagttctca gcaattctca ctggacgaca atagacgtaa aaaagcccgc agagcttgtg
     2521 ctgtgtgggc ttagtagact tcattgaact tcaaacaact aaaaagtggt ggagctggcg
     2581 ggagttgaac ccgcgtccga aattcctaca tcctcggtac tacatgctta gtcagtcttt
     2641 acattcgctt gccagctgcg gacggacacg ccactaacaa actagcctga ttaagtttta
     2701 acgcttcaac cccaggcagg gcttccacgc gatctctttt gggtttgacc tctcttgatc
     2761 cccgtcctaa gagcggaggc tagggagaga gggctctaag caggttatta agctgctaaa
     2821 gcgtagtttt cgtcgtttgc gactattttt tgcggctttt tacgaggcca accgcccctc
     2881 ggcatgcacc ttgggtttcg caaatcccgt cgaatccaga atcagcccca atgtgtaaag
     2941 gtaagtatac cagatttatg agcgccatga ccagcctcaa tggcgttatc gttaaagatt
     3001 tagcacccat gtagcctgat ttttattcga ttaagcaatg ggatggcaac atttgtgtcg
     3061 gatgtgatag ccaataagat gttcattcgc gccgccggag agggaggcgc ggtgaggaac
     3121 tggtcaataa ttggagtgca ggtttaacgg tgggcgtttt tcatgatacg tgctttatcc
     3181 acctgccatt cgcgttcttt gatatctgaa cgtttatcgt gctgtttttt acctttggcg
     3241 acgccgattt tcactttgca ccaggcattt ttccagtaca gggagagcgc cactacggta
     3301 tagccttctc gattgacgcg accgtacaat gagtccagtt cgcgttggtt gagaagtaac
     3361 ttgcgggtac gggtaggatc gcacaccaca tgcgtggagg ccacggccat tggcgtgatg
     3421 ttagcgccaa acagaaatgc ctctccgtca cgcagaagga cgtagctgtc gctgatattg
     3481 gcttttcctg cgcgcaggga tttaacctcc cagccttgca gggcaagtcc cgcttcgaac
     3541 tcttcttcga taaagtattc gtgacgggcg cgcttgttaa gcgcgatggt cgctgaacca
     3601 ggtttatgtg cttttttctt cgtcataagc gtcgtgaatc atcggtaatc tgaaatcgaa
     3661 aacaccccat atcaatcctg cgggggacaa ggctctatct tagcatgaac ccgatgttac
     3721 ccagcgccgg gatagcgttt tttttacagc aggataaatg atattatttg ttggattttt
     3781 gttgatggaa attgttatgc ctcagattag ccggaccgca ctggtaccct acagcgcgga
     3841 gcaaatgtat cagttagtga atgacgttca gtcttatcct cagtttttgc cgggttgtac
     3901 cggaagtcgg attctggagt ccactcctgg gcagatgact gcggcggtag atgtctctaa
     3961 ggctgggatc agcaaaacgt ttactacccg caaccagttg accagtaacc aaagtattct
     4021 tatgaatctg gtggatgggc cgttcaagaa attgattggt ggatggaagt ttacgccgct
     4081 gagccaggag gcgtgtcgta tcgagtttca tctcgacttt gagtttacca ataagttgat
     4141 tgaactcgcc tttggtcgcg tgtttaaaga gctggcgtct aatatggtcc aggcttttac
     4201 ggttcgtgcg aaagaggttt acagtgccag gtaaaattgc cgttgaggtg gcttatgcgc
     4261 tacctgagaa gcagtacctg cagcgcgtga cgctgcagga gggcgcgacg gttgaagaag
     4321 ctattcgcgc cagtggcttg ctggaattgc gtaccgatat cgatttaact aaaaacaaag
     4381 tcggcattta cagccgtccg gcaaaactaa gcgatagtgt gcatgatggc gatcgggtgg
     4441 agatttatcg ccctctcatt gccgatccga aagagcttcg caggcaacga gcagaaaaat
     4501 cagcgaataa ataacagaca gaaaaaaggt gctcattgag cacctttttt aacgtctttg
     4561 agagcaactt tattattagt taccactcag cgcaggtttg ttatcaatat tggtcaacac
     4621 accgctactg ttaaaggtca gcgtcagcgt ttgctgcgtt acaccttcat gacctggttg
     4681 ctggcggaag acatagaacc aggtattcgt accaaatgga tcggacatca gcggtgtacc
     4741 caatgcgtac gcaacttgtt gttgcgtcat gccaacacgt attttggata cgtcgttagc
     4801 ggtcagatag ttcccctggt tgatgtcagg acggtaaacc actcgctcca gagtggaaca
     4861 gcctgcggtc aacatcaata gtactgctgc tgcagcagtc agcgttttac agcgcatagt
     4921 gatttgattc cttttcgggc ccgagcagta cgtggctcat ctgtaatatg ccgatgataa
     4981 tagacctttc atcactttaa aaccttttgc tttccggtct tacggcgttt tgctgtttac
     5041 tctgaccgtg aagcaggaaa aaagtttacg ctgcaagcag ttctttcgca ttcgccagtg
     5101 tattacgtgt gacttcactg ccaccaagca ggcgcgccag ctcttgtaac cgcgcttttt
     5161 tattcaggga ttgcatatgc gtttctgtca tcgcaccatc ggtttctttg ctgacaaaat
     5221 agtgttgatg accacatccc gcgacttgtg gcaggtgggt aacacacatc acctgagttg
     5281 attcgccgag ttgacgcagc agtttgccga caactgccgc tgttggaccg ctaataccta
     5341 catccacttc atcaaaaatc agtgccgggg tttccatttt acgcgccgtg atgacctgga
     5401 ttgccagtgc gatgcgggac aattcaccac cggatgcgac tttggcaata ggctgcattg
     5461 gctgacctgg gttggtggtt acccgaaact caatacgatc ggcaccgtca gcgcccaggt
     5521 gatgctcgtc aaatttaaca tcgatcgtaa actgcccatg cggcattgag agcgcatgca
     5581 tactgtcggt gatcagctgt gcaagttctt ctgcatattg ctggcgttgt tggtgtaatg
     5641 cgcgcgcgat ttccagtgcc tgctgatgat gtttcgttac cgccagcgca agcgtttctt
     5701 gtgagtcggc ctgatcgtcc agttgctgct gttcttccag tagcgactgg taatactgtg
     5761 gcaatgcctc agggctgacg tgatgtttac gtgccagcga aatctgtttt gagatgcgct
     5821 gttcaagttc aaatagtcgg ttgggatcga gatccagacg atcgcagtag tggcgcagtt
     5881 catcgctggc ttcagcaatc tggatggtag cttcttccag catatcaagt acgccggaca
     5941 gtttgctgtc catgccaatc aattcgctca ccagttgttt agccgtgtaa agctgacttt
     6001 gcaggtttgc gtcttcaccg tcggccatta atgccaatgc attctggctg gtggtcagca
     6061 attgaccgct gttcgccaga cgtttgtact cttcgtcgat ttgttcaaac tctccgggct
     6121 gcggattaaa ttcgttaagt tcttttaatt ggtattgcag cagttccgca cgggcagcgc
     6181 gttcctgact taactgttga tgatgcgcga ggtcacggca gctttgatgc cacaactgat
     6241 aacgtgctgt catttcctgg agtagagagg tttcattggc atagccatca agcaggaact
     6301 tttggtgctc gggtttggtg agtaactgat gagcgtgctg accatggatc tgaatcagca
     6361 actgacccag ttcgcgcagt tgtgacagag gaacagctgt accgttgatg aaaccacggg
     6421 agcgaccatc gctgctgatc acgcgacgaa gcaaacattc atgcccgtct tcaagctggt
     6481 tttcttccag ccagcgcaga gccgctggcg tatctttcag agaaaaacgg gcgcacaggt
     6541 cagcgcgagc agcgccggta cgcaccatgt cggcttcagc gcgaccaccg agacaaagac
     6601 cgagggcatc tattgcaata gatttacccg cgccggtctc gccagttatt acggtcatgc
     6661 cgctatgaaa atcaatctca agctcacgaa cgatagcaaa gttgctgatg gtcagttgtg
     6721 ccaacatagt cgttttcctg tatgaaaaac cattactgtt attgtgtaca gtataaactg
     6781 gttttatata cagtaaagag gctggcgtaa aattagaata atttttttga ccagccgagc
     6841 ttggtgctta atgtgttgaa ataactgtaa tctttcggat gaatcagatt cagatggtaa
     6901 tcacagcgac gaatcaggac atcttcacct tcctgaatcg gcagtgctat ctggctgtcg
     6961 caactgattt ccaggtcgtt acggcgatgc gaaaaacgca gacggatcgt gctgctgctg
     7021 tttatgacca gtggtcgtgc tgacaacgta tgcgggaaca tgggcaccag ggtaatcgca
     7081 tccagagagg gggtcagaat aggaccgcct gcagagaggg aataggcggt ggagcctgtt
     7141 ggcgtcgaaa taattagtcc atcagatcgc tgagaaaacg caaagatctc gtcgatatac
     7201 acttcgaact caatcatatg cgccactttg cctggatgaa gcaccacttc atttatcgcg
     7261 gtgctgatgc gtttctggca atcttgctga cagacttgcg cttccagcaa aaaacgtttc
     7321 tcgctgatgt agtggccttc cagcacatcg gctaactgtt gctgggcgtt atcggggtca
     7381 aggtcagtca ggaaacccag gttgccacgg ttgattccaa taactttaat atcgtaacgg
     7441 gcgagtgtgc gtgccgcgcc cagcatatta ccgtcgccac caacgactac cgcgagatca
     7501 gctagttgcc caatctccgc gagcgtgcca gttttcacat tcttcagttg cagttcgtga
     7561 gcgatttgtt gctcaacgat gacctcgtaa ccttttgtgc acagccagcg gtagagcatt
     7621 tcatgtgttg tcagtgcagt ggggtgccgt gggtgtccca caatgccaat acacttgaaa
     7681 tgattattca tttttccgag gtccttgtta cgaagattga tgacaatgtg agtgcttccc
     7741 ttgaaaccct gaaactgatc cccataataa gcgaagttag cgagatgaat gcgaaaaaaa
     7801 cgcggagaaa ttcatgagta gtaaagaaca gaaaacgcct gaggggcaag ccccggaaga
     7861 aattatcatg gatcagcacg aagagattga ggcagttgag ccagaagctt ctgctgagca
     7921 ggtggatccg cgcgatgaaa aaattgcgaa tctcgaagct cagctggctg aagcccagac
     7981 ccgtgaacgt gacggcattt tgcgcgtaaa agccgaaatg gaaaacctgc gtcgacgtac
     8041 tgaactggat attgaaaaag cccacaaatt cgcgctggag aaattcatca acgaattgct
     8101 gccggtgatt gatagcctgg atcgtgcgct ggaagtggct gataaagcta acccggatat
     8161 gtctgcgatg gttgaaggca ttgagctgac gctgaagtcg atgctggatg ttgtgcgtaa
     8221 gtttggtgtt gaagtgatcg ccgaaactaa cgtgccgctg gacccgaatg tgcatcaggc
     8281 tatcgcaatg gtggaatctg atgacgttgc gccaggtaac gtactgggca ttatgcagaa
     8341 gggttatacg ctgaatggtc gtacgattcg tgcggcgatg gtaactgtag cgaaagcaaa
     8401 agcgtaattt ctgttttcgt aataattcac ggccctgcat gcgaatgcag ggccgttttc
     8461 gttactccgc cacactctcg cgcagcggtt ttacaggaaa gacttttacc tgcttaatca
     8521 tattgtcctg aacgtcgaga atatcgatat cgtactcgcc aatatgcacg cgggtgccag
     8581 cgacagggat ctcttccaga gcttcaagaa tgacgccatt aaccgtgcgg gcatcgtctt
     8641 caggtagatg ccagttaaag gctttgttga tttcccgcac gttggcggtg ccatcgataa
     8701 tcaccgaacc gtcgttttgc ggcgtgacct cttcggcaag tgttggcgac atcgacgtag
     8761 tgaaatcgcc gacaatctct tccagaatat cttcaaccgt caccagcccc tgaatgtctc
     8821 catactcgtt gacgaccagg ccgactttct ttttgttgcg ctgaaacttt accaactgcg
     8881 tgctgagcgg cgtaccttcc ggcacaaaat agatctcgtc cgcggcgcgc agcatggttt
     8941 ctttggtgaa ctcttttttc tccgacatca accgccaggc ttcacgtact cgcagcatac
     9001 tgatggcgtc gtccagcgaa tcacggtaga gcacgatgcg cccgtgaggt gagtgggaga
     9061 gttggcgcag aatcgatttc cagtcatcgt tgatatcaat accgataatt tcactgcgcg
     9121 gcaccatgat gtcatcaacg gtcatttttt ccagatcgag caccgacagc agcatatcct
     9181 gattgcgacg ggaaatttgt gagcgtgatt cgtgcacgat agtgcgcaac tcttctttgc
     9241 tcaaagagcc gctaaccacg atatcggttt tgatacccat catgcgcatc agcatacggg
     9301 tgatagcatt cagcaaccag accagcggca tcatcaaaat ttgcagcgga gccagcagaa
     9361 aactactcgg ataagcgact ttttccgggt acagcgcggc aatggttttc ggcaatacct
     9421 cagcaaatac cagtacgaca aaagtcagca caccagtcgc aattgccacg cccgcatcgc
     9481 cgtacaaacg catcccaaca atagtgccga gcgcggaggc aagaatattg accaggttat
     9541 tgccgattaa caccaggctt atcaggcggt ctggcttacg cagcaatttt tcgacgcgtt
     9601 tggccgagcg attaccctgt ttcgccatat gtcgcagacg atagcggttg agggtcatca
     9661 ttccggtttc ggacccggaa aaataggctg aaatgaccac catgatgatc agaataatga
     9721 tcaacgtagt agtagaaatg tgttccaggg gaaactcctt ttctgggttt agctgattaa
     9781 ctgctggaca attcggctgc cgaagtaggc cagtgtcaga attaccgcgc ccgcaacgtt
     9841 aaaccagacg acgcggcgtc cacgccatcc ttcatggtaa tgtccccaca gcagcacaat
     9901 atagacaaac cacgccacga tagagagcac agccttgtcg atattttcca tgctaaatag
     9961 gttgtgcatg tagaacaggc cagtgcaaag cgtgagcgtt agcagcacca cgccaatctg
    10021 cgtgatgtgg aacattttac gctcgatact catcaatggc ggcatttcct ggttaaatgc
    10081 cagcttcttg ttcttcagtt ggtaatcaat ccacgccagt tgcagcgcgt acagggcggc
    10141 gataattagc gtggcatagg aaaagagcga taagccaatg tgcaccagca tcccaggcgt
    10201 tgcttccaga tgggtgatgt attcattggg catgaaggtc gccagcgcca ggttgataag
    10261 cgcaaaggca tagacaatgg gtagcagcaa ccagccacga ttgcgagaag ccacaatggt
    10321 cattaccgta cagatcatca aactgaccaa tgaaccaacg ttcagcaggc tgaggttttg
    10381 tccgctatca ccgtcgggca ggatgcgggc ttccagagcg attgcgtggc agaccagcgc
    10441 aatgaccgca gaaataatag ccatgcgccg ccagccgccg tttttttgca gcagaccggg
    10501 aacaatcagc gcaagactga cggagtaggc gacaagcgcg agcagagcaa aaacgggcat
    10561 agtgatgtcg acagtttgag caataagaaa gagaagcagt atagcgtcag gtgaacgctg
    10621 ctccaaccgt tgcataacaa caaagacgcc ttcatgttat actgcggcaa aatactgatg
    10681 atgtgtcgcg attgcggcca accgtttcca ccccaggcga gagacaatgt ttgataattt
    10741 aaccgatcgt ttgtcgcgca cgctgcgcaa tatcagtggc cgtggacgcc tcactgaaga
    10801 caacgtaaaa gatacgctgc gcgaagtgcg catggcgctg ctggaggcgg acgtcgctct
    10861 gccggtagtg cgtgagttta tcaatcgcgt aaaagagaaa gcggttggtc atgaagttaa
    10921 taagagcctg acgccggggc aggagttcgt caaaatcgtc cgtaacgaac tggttgcggc
    10981 gatgggcgaa gagaaccaga ccctgaacct ggcggcgcaa ccgcctgcgg tcgtgctgat
    11041 ggcgggcctg caaggtgccg gtaaaacaac cagcgttggt aaactcggta agttcctgcg
    11101 cgagaagcac aagaagaaag tgctggtggt ttctgccgac gtttatcgcc cggcggcaat
    11161 caaacagctt gagacgctgg cagagcaggt gggcgttgat ttcttccctt ctgatgttgg
    11221 tcagaagccg gtagatatcg ttaacgcggc gctgaaagaa gccaaactga aattctacga
    11281 cgtgctgctg gtggataccg ctggtcgtct gcacgttgac gaagcgatga tggacgagat
    11341 caaacaagtc catgcgtcga ttaacccggt tgaaaccctg tttgtggttg acgccatgac
    11401 cggtcaggat gcggccaata cggcaaaagc attcaatgaa gcgttaccgc ttaccggcgt
    11461 agtgttgacc aaagtggacg gcgatgcccg cggcggtgcg gcgctctcta ttcgtcacat
    11521 cactggcaaa ccgatcaagt ttctcggtgt tggcgagaaa actgaggcgc tggagccgtt
    11581 ccatccggac cgcatcgcgt cgcgtattct cggcatgggc gacgtactgt cgctgatcga
    11641 agatatcgaa agcaaagttg accgcgcgca ggcagagaaa ttagccagca agctgaaaaa
    11701 aggcgacggc ttcgatctca acgattttct tgagcaactg cgccagatga aaaatatggg
    11761 cggcatggct agcctgatgg gcaagctgcc gggcatgggg cagatcccgg ataatgttaa
    11821 gtcgcagatg gacgataaag tgctggtgcg tatggaagcc atcatcaact cgatgacgat
    11881 gaaagagcgc gctaagccag aaatcatcaa aggttcgcgt aaacgccgta ttgctgccgg
    11941 ttgcggtatg caggtgcagg acgttaaccg tcttctgaaa cagttcgacg acatgcagcg
    12001 catgatgaag aaaatgaaga agggcggaat ggcgaagatg atgagaagca tgaaggggat
    12061 gatgccccca ggcttccctg gtcgctaatc ggcctggctt gtttgccatt ttggcaccgg
    12121 gatgtgctcg tcatcctcac gtactgcgtg tacgctccgg tgactgcgcg cactccggca
    12181 ccaaactggc tgcaccgccg acggcctttt acagcagagt tagcaactgt tgattgcaat
    12241 tcccgcaaaa atgagtaaaa ttttcgggct tttaatatga caccggactc cgttcctcga
    12301 tggggtccgg ttgttttatt cacacaagag gatgttatgg taactattcg tttagcacgt
    12361 cacggcgcta aaaagcgtcc gttctaccag gttgttgtcg ctgacagccg taatgcacgc
    12421 aacggtcgct tcatcgagcg cgttggtttc ttcaacccaa tcgctagcga aaaagaagaa
    12481 ggcactcgcc tggatctgga tcgcatcgct cactgggttg gccagggcgc aactatttct
    12541 gatcgcgttg ctgcgctgat caaagaagta aacaaagcag cttaatctgt cacggtggtc
    12601 atgatgagca aacaactcac cgcgcaagca cctgttgatc ccatcgtttt gggaaaaatg
    12661 ggttcgtctt acggtattcg tgggtggctc agagtgtttt cttccaccga agacgccgaa
    12721 agcatttttg actatcagcc ctggtttatc cagaaggcgg gtcagtggca gcaagtccag
    12781 ctggaaagct ggaagcacca caatcaggac atgatcatca agctgaaagg cgttgacgat
    12841 cgtgatgcgg cgaacctgct gacgaattgt gaaattgtcg tggattcatc gcagctgcct
    12901 cagcttgaag agggtgacta ctactggaaa gacctgatgg gctgccaggt agtaaccact
    12961 gaaggctacg atctcggtaa agtcgtcgat atgatggaaa ccggatctaa tgacgttctc
    13021 gtcattaagg caaacctgaa agatgcgttt ggtatcaagg aacgtctcgt accgttcctc
    13081 gatgggcagg ttatcaagaa agtcgatctc actactcgtt caatcgaagt agattgggat
    13141 cctggttttt aaaccaccgg ataaacggta aaagacggcg ctatgtggat tggcataatt
    13201 agcctgtttc ctgaaatgtt ccgcgcaatt accgattacg gggtaactgg ccgggcagtt
    13261 aaaaatggcc tgctgagcat ccagagttgg agtcctcgcg acttcacgca tgaccggcac
    13321 cgtaccgtgg acgatcgtcc ttacggcggc ggaccgggga tgttaatgat ggtgcaaccc
    13381 ttgcgggacg ccattcatgc agcaaaagcc gcggcgggtg aaggcgcaaa ggtgatttat
    13441 ctgtcaccac agggacgcaa gcttgatcaa gcgggcgtca gcgaactggc aacgaatcaa
    13501 aaattgattc tggtgtgcgg tcgctacgaa ggtatagatg agcgcgtgat ccaaaccgaa
    13561 attgacgaag aatggtcaat cggcgattac gttctcagtg gtggtgagtt accagcaatg
    13621 acgctgattg actccgtttc ccggtttatt ccgggagtac tgggacatga agcctcggca
    13681 acggaagatt cctttgctga aggattgctg gattgcccgc actatacgcg gcctgaggtg
    13741 ttagaaggga tggaagttcc gccagtgtta ctgtcgggca accatgccga gatacgtcgc
    13801 tggcgtttga aacagtcgct gggccgtacc tggcttagaa gacctgaact tctggaaaac
    13861 ctggctctga ctgaagagca agcaaggttg ctggcggagt tcaaaacgga acacgcacaa
    13921 cagcaacata aacatgatgg gatggcgtaa gcccccgaga tatcagttta cccaggataa
    13981 gagattaaat tatgagcaac attattaagc aacttgaaca agagcagatg aagcaggacg
    14041 taccttcctt ccgtccgggt gataccgtgg aagtgaaagt atgggttgtt gaaggttcca
    14101 aaaaacgtct gcaggcattc gagggcgtgg ttatcgctat tcgtaaccgc ggtctgcact
    14161 ctgcattcac tgttcgtaaa atttccaacg gcgaaggcgt tgagcgtgtc ttccagactc
    14221 actctccggt agttgacagc atttctgtca aacgtcgtgg tgctgttcgt aaagctaaac
    14281 tgtactacct gcgtgagcgt actggtaagg ctgctcgtat caaagagcgt cttaactaag
    14341 atttcgctta agcgacatcc tgttaagaag ggctggccaa ttggctggcc ctttttttgt
    14401 ctgtttgctc gtgttttaag gggttgtaat caccactgcg acgcggcggt tctcggcgcg
    14461 gccctgggcg gtcttgttac tggctatggg atattttttt cctaaaccct gtgtggtgag
    14521 attgctgcgt ggaatttgtc cacccatagc ccatgcatcg gccacgacat tcgcccgttt
    14581 caatgataag ccttcgttgt aactgtcttc accatagtta tcggtgtgtc catccatacg
    14641 ggcatgtgtt agccctgtcg aggccaattt ggctgccatg gtttggatct gttgctggct
    14701 ttccgggagc aatttgtagt cattttttgc gaacagaatg gcatctgata agcccagcga
    14761 ccagtcgccg gcggattcag taaatccata agattgcata gcggcgactt gctcaggagt
    14821 aaactttccc tgaggggact ggcagccagt cagtattagt gaggtgaaaa tcaggggtgc
    14881 taccaggtgc tttatcatat cgatatatcc ttgttatctc accagctttt cggcacgctg
    14941 gtgtttggcc tgatacatat tgtgatcggc aagctcttgt aatttttccg cagaggcgtg
    15001 ctcaatggtc atcgcgtaac caatgcttaa tgtcatggtg gtctgatggc cattatgaag
    15061 atcaaacggg agattaaaga tttgtgtcag tgctgagcat atctgctgca cttcagattc
    15121 cgactgtaca tcatagagca ccatagcgaa ttcatcgccg cccaggcggt atgctttatg
    15181 tcgcagcccg ccaaattcag ctaaccgttt tgcgatttca atcaagactc tatcgcccgt
    15241 cgcatgaccc caggtatcat tgatatattt gaaattatcg ccatcaagaa atagtaacgc
    15301 cgacgttttt cgggcatcgg aattgttcat caacgtattg atgccgctac gaaacgctgc
    15361 gcggttagcc agcccggtta atgggtcatg tagcgcggta cgcagaagct gcgcattttt
    15421 ggcctgtaaa cgaagctgcc actcttccat ttcatccagc agactgttga agtcgagagc
    15481 gaagcggtga aactcagcga tacgttcttc cgaaactcgt cgggaaaaat tgcggttgga
    15541 acgcacatca tgtacgacat cggtgatatt tttcagcgct tctaccaggc cattgtgcaa
    15601 atggcgggtg agggtaattg cgatgcctga tgccagcaga atacaaccgg tcagtacggc
    15661 gagcgaaaac cagataaaat ggctgattga actgtcgcga gcggttaagc gtacttcgcc
    15721 aatggtttca ccattgtgac gaatcggctg aatgatgggg gcggggaaga gccagtggct
    15781 tatgaaattg ctgaaagtat cgcctggatc cttacgggtg taatgccagg acgccagaat
    15841 attttgctgc ttatcacgta cttctgcagt tgaaaattgc ccttgctggc ccagcgctgc
    15901 cagtgtttca gttgctgcag ggccatcggc aaaaacgaca gctgcttcca gactgtaagt
    15961 cattgttgct gctgtcagtg ccaggttttt ttgcgcgtac tgtttcaggg tcagcaccga
    16021 ggtcacggaa agcagcaacc agatcagcat catagtgata aatatgctgg tcatactgat
    16081 gttgcgtaat gctcttttaa acgtggggcg cttattaaga gaattatcgt tatccatcat
    16141 ttttcttccg tgcgagcatt aagacatccg ggttgacctt tacaccgcta cgcgataagg
    16201 catccaggtt tacggcaaat ctgacgtcat tattgtggat gatcagacaa aaggcgctac
    16261 caataatgca ctcggtattt tgttcggcaa ttaataataa cgcctttgac ggatattgtt
    16321 cggttaattc catctggaat gtcggtgact cgttgccaaa ataaaaacca ttgcagcctg
    16381 aaatcatcgc ctcttgttga gtgtgaatga tgacaggcag ataaggcaaa gatgtcgcgg
    16441 cattctcttg tagggcggta ctaaaacgtg aagatgaaaa gatacataac tttggtggcc
    16501 ctgatagcgc aggccagcgg gtgtaactga caataccgga aaccatcata cgcacgtttt
    16561 tcgcaacgtc agaaggctcc tgagcgagta ttggtgctcc cgtcagcagg agaatcagaa
    16621 gtaagaacag tcggtgagaa aaacgcattc agggatcccc accatcttcg ctcgaaatgc
    16681 atcgatattt tttcagaatg ctcattaaaa tatcattttt tcatgcgatc gtcatcaatg
    16741 tgagaaataa aacacaatga gtaaaatctt atgtttaatt tatttgtggg gttttaatta
    16801 atttaatcgt attgtgctgg cattttcatc agcccctttt tgccatactt catgcagttg
    16861 gctggcattt tcaacattgt tgcagctggc aggaaagctt ttaccggaaa gtccccgggc
    16921 ataagtaaaa tcggtctggc aagttttctt ttgtccttcg gcatatcctt taaggtatag
    16981 accgcgatcc gcctgtgaat cgctaaatgc atcgtcatct tttatggcgc tgcccgatat
    17041 cgcatcttcc ataccaacat cataccagtc ggtgctggtc aaggttggcg cgtgagtata
    17101 aggatcaatc tgacatccgc taaccagtaa agccaacaag ggggcgataa actttttcat
    17161 catttctttc tcctttttca aagcatagcg gattgttttc aaaggaagtg taaatttatc
    17221 tatacagaag taagggttga aatcgcgact aaattgcctg tgtaaataaa aatgtacgaa
    17281 atatggattg aaaactttac tttatgtgtt atcgttacgt catcctcgct gaggatcaac
    17341 tatcgcaaac gagcataaac aggatcgcca tcatgcaaaa agacgcgctg aataacgtac
    17401 atattaccga cgaacaggtt ttaatgactc cggaacaact gaaggccgct tttccattga
    17461 gcctgcaaca agaagcccag attgctgact cgcgtaaaac catttcagat attatcgccg
    17521 ggcgcgatcc tcgtctgctg gtagtatgtg gtccttgttc cattcatgat ccggaaactg
    17581 ctctggaata tgctcgtcga tttaaagccc ttgccgcaga ggtcagcgat agcctctatc
    17641 tggtaatgcg cgtctatttt gaaaaacccc gtaccactgt cggctggaaa gggttaatta
    17701 acgatcccca tatggatggc tcttttgatg tagaagccgg gctgcagatc gcgcgtaaat
    17761 tgctgcttga gctggtgaat atgggactgc cactggcgac ggaagcgtta gatccgaata
    17821 gcccgcaata cctgggcgat ctgtttagct ggtcagcaat tggtgctcgt acaacggaat
    17881 cgcaaactca ccgtgaaatg gcctccgggc tttccatgcc ggttggtttt aaaaacggca
    17941 ccgacggcag tctggcaaca gcaattaacg ctatgcgcgc cgccgcccag ccgcaccgtt
    18001 ttgttggcat taaccaggca gggcaggttg cgttgctaca aactcagggg aatccggacg
    18061 ggcatgtcat cctgcgcggt ggtaaagcgc cgaactatag ccctgcggat gttgcgcaat
    18121 gtgaaaaaga gatggaacag gcgggactgc gcccgtctct gatggtagat tgcagccacg
    18181 gtaattccaa taaagattat cgccgtcagc ctgcggtggc agaatccgtg gttgctcaaa
    18241 tcaaagatgg caatcgctca attattggtc tgatgatcga aagtaatatc cacgagggca
    18301 atcagtcttc cgagcaaccg cgcagtgaaa tgaaatacgg tgtatccgta accgatgcct
    18361 gcattagctg ggaaatgacc gatgccttgc tgcgtgaaat tcatcaggat ctgaacgggc
    18421 tgctgacggc tcgcgtggct taagaggttt attatggttg ctgaattgac cgcattacgc
    18481 gatcaaattg atgaagtcga taaagcgctg ctgaatttat tagctaagcg tctggaactg
    18541 gttgctgaag tgggcgaggt gaaaagccgc tttggactgc ctatttatgt tccggagcgc
    18601 gaggcatcta tgttggcctc gcgtcgtgca gaggcggaag ctctgggtgt accgccagat
    18661 ctgattgagg atgttttgcg tcgggtgatg cgtgaatctt actccagtga aaacgacaaa
    18721 ggatttaaaa cactttgtcc gtcactgcgt ccggtggtta tcgtcggcgg tggcggtcag
    18781 atgggacgcc tgttcgagaa gatgctgacc ctctcgggtt atcaggtgcg gattctggag
    18841 caacatgact gggatcgagc ggctgatatt gttgccgatg ccggaatggt gattgttagt
    18901 gtgccaatcc acgttactga gcaagttatc ggcaaattac cgcctttacc gaaagattgt
    18961 attctggttg atctggcatc agtgaaaaat ggaccattac aggccatgct ggcggcgcac
    19021 gatggcccgg tactggggtt acacccgatg ttcggcccgg acagcggtag cctggcaaag
    19081 caagttgtgg tctggtgtga tggacgtaag ccggaagcat accaatggtt tctggagcaa
    19141 attcaggtct ggggcgctcg gctgcatcgt attagcgctg tcgagcacga tcagaatatg
    19201 gcgtttattc aggctctgcg ccactttgct acttttgctt atgggctgca tctggcagaa
    19261 gaaaatgttc agcttgagca acttctggcg ctctcttcgc cgatttaccg ccttgagctg
    19321 gcgatggtcg ggcgactgtt tgctcaggat ccgcagcttt atgccgacat tattatgtcg
    19381 tcagagcgta atctggcgtt aatcaaacgt tactataagc gtttcggcga ggcgattgag
    19441 ttgctggagc agggcgataa gcaggcgttt attgacagtt tccgcaaggt ggagcactgg
    19501 ttcggcgatt acgcacagcg ttttcagagt gaaagccgcg tgttattgcg tcaggcgaat
    19561 gacaaccgcc agtaataatc cagtgccgga tgattcacat catccggcac cttttcatca
    19621 ggttggatca acaggcacta cgttctcact tgggtaacag cccaatacct tcattgaacg
    19681 ggtaatttcc cctaactctt tcaatgcttt ttgcatttcc gctgattcaa gattggcctg
    19741 aatatccaga taaaacatct cttcccatgg attaccgtga atcgggcgtg attccagacg
    19801 ggtcataatc agattgtggt tgcgcagcac cagcaacgct tcaaccagcg caccggcttg
    19861 ttgcccggtc gccattaaca acgtcgtttt cgccggaacc tggtcagaca cgttaatggc
    19921 tttacgtgcc aacaccacaa atcgggtgaa gttttgttgc tgattcgctt caatacgctc
    19981 cagtacctgc aaaccgtaca aagtgccgcc agcttcgctt cccaatgcag caacatgcgg
    20041 tgattttgcc tgtgcaacct tttccattgc cgcagacgta ctttcggtat attcaatctt
    20101 ccagtgagga taacgattaa ggaatttgct gcattgctgg aatggctgtg gatgactgta
    20161 gaccgtgttg atggtagata aatctgtggt gccggaaacc agcaggcagt ggtcgatggt
    20221 aagggtcatt tcgcccacaa tcgacaagca ggtatgttgc agcagatcgt aaacgtcgtt
    20281 tatggcaccg gagctggtat tttcaatcgg tacgacggca tagtcggcct ggccggtttc
    20341 cacctgatta aaaatatcgg caaatttggc gcagccactt tcaatgaatt gctcaaagtg
    20401 acgggcagcg tactgacgag cggcaagatg tgaataggag cctttggggc cgagaaaagc
    20461 gatgcgtgct gagtgcggat taattttatt gagatgttgt tggagcaaag cctgctgagt
    20521 taatacggaa tcttcaatga tgagctggaa caggcgagta atgtaatggg cgtccagatg
    20581 gtgcgcttta ccgagcgtaa ttaatctttc cagtaaatcg cgttcacgat caatatcacg
    20641 taccgggcga tgcgagagca gtttggcttt tcccacctcg acggccagtt cgcgccgctc
    20701 tgcgagtaat gctaataatt tttcatccag cgcgctgatt ttctctcgca gcgccagtaa
    20761 cgggttttcc gatgtcatag tgttgccttt ttgttatcaa taaaaaaggc cccccgattt
    20821 gggaggcctt attgttcgtc ttcgcattct gtttcacacg acgaaacgcc tccctttcag
    20881 gggaagataa aaaagaatgc gaagaaaaac ggtgtgtgtt tcatgtttgt ttccttaagt
    20941 gactttagta cagtacccgt actgttttca cgctgtcaac aaaaaacgcg cccgaaggcg
    21001 cgttggcgat acactcaata taaaggacta ctcttcttca acttcttcga cgaagttggc
    21061 gtctttcacc gatgttgcgg cacgacgtgc ttcgcctttg tgctgcagtt tattgagctg
    21121 ccgttccagc ttgttgatca attcgttaat tgcggtgtac atatcttcat gtttaccact
    21181 ggcaaccaga acgccgttag gtgtattgat tgtggcgtca gcaacaaacc cttgtggctc
    21241 tttggacaga atgatatgtg gattaatcag atgtgtttgc catttttcca gtttggcgag
    21301 acggtctgcg acatgttggc ggatggccgg agtaatttcc atttgtttgc tggtaatgtt
    21361 cattgtcata aattttacct cttgtcttcc cgtcttggtg aattcagcat accgttcctg
    21421 atgtcaaaat gtgtgatgaa aatctcataa ttttgtcact ttttgtcaac gaatcttttt
    21481 tgtgagaaat cgcaggaaga ggtgtttttt tacttgagga atatctcaaa gcgagccata
    21541 tttgatgaga tcgatagcga ctaaatcggt tcagtttcac aactgacaga ataaaaaaac
    21601 ggcagctcaa gggctgccgt tttgtgtttc aggtttctgt tatgtattgc tgctgtttgc
    21661 ggcgatgatt ttcgctactt tttcagcttg cgcattcatc tgcatctgac ggtatgcatt
    21721 ttccatcagc ggcagcgcat cacgcgtagc ctgggtatcc gggtagtcgc gcaacatgcc
    21781 ttctacgcgg ttaacgacgg caacccatgc gccacgttct gtatagtact cggccacgga
    21841 gtattcatat ttcgccagac gatctttcag gaataccaga cgtttggtgg catcggtggt
    21901 gtactgactg ttcggatagc cgcgcaccag tttggaaaag tcactaaacg cagctcgtgc
    21961 atgttgagga tcgcgatcgc tacgatcgac gccaaagaac ccttgcagcg cactgtcatc
    22021 cagcgccata ttggtcaggc cacgcatgta catgacataa tcgatattcg gatgggtcgg
    22081 gttaaggcga ataaaacgat cgatggcagc ctgtgctaac ggcaaatcgg cgtttttata
    22141 gtaggcgtag atgagatcca gctgcacctg ctgcgaatat ggaccaaacg gatagcgatt
    22201 atctaacgct tccagttgcg ttattgcctg tctccagtta ccgtcctgca gcttttgttg
    22261 tgcagtcgcg taaatttcat ttggcggatt atcaggtact tcttcctttg accccgagca
    22321 acccgccaaa aacaggctta gtgtggcggc tgccaccaga tatttcatgc gcgtcatgac
    22381 gttttgactt tcctcaaaat gtaatacggg agattctctg ttcctgctcc cggttaagac
    22441 cagctacaat agcacactat attaaacggc aaagccgtaa aaccccaacg ataaacgaag
    22501 aagcagtata tatggcacaa cgagtacagc tcactgcaac ggtgtccgaa aaccaactcg
    22561 gtcaacgctt agatcaggct ttggccgaaa tgttcccgga ttattcacgt tcgcgaataa
    22621 aagaatggat cctcgaccag cgcgtgctgg taaacggcaa agtttgtgat aagccgaaag
    22681 aaaaagtatt gggtggcgag caggttgcca tcaacgctga gattgaagaa gaagcgcgtt
    22741 ttgaaccgca ggatatcccg ctggatatcg tctatgaaga tgaagatatc atcgtcatta
    22801 ataaaccgcg cgacctggtg gtacatcctg gcgcgggtaa cccggatggc acggtactga
    22861 atgcgttgct tcattactat ccgcccattg ccgatgtacc acgtgcgggc atcgtccatc
    22921 gtctggataa agacaccact ggcctgatgg ttgtggcaaa aaccgttccg gctcagacgc
    22981 gtttagtcga atctttgcaa cggcgtgaaa ttactcgtga gtatgaagcg gtggcgattg
    23041 gtcatatgac cgctggcggt acagtagatg aacccatcag ccgccacccg accaaacgta
    23101 ctcacatggc ggtgcatccg atgggcaaac cggcggtgac tcactatcgc atcatggaac
    23161 acttccgtgt gcacacgcgt ctgcggttgc gtctggaaac tggacgtacg caccagatcc
    23221 gcttgcatat ggcccatatc actcatccgc tggtgggcga tccggtttat ggtggccgtc
    23281 cgcgtccgcc aaaaggtgct tcggaagcat ttatctccat gctgcgtaag tttgaccgcc
    23341 aggcgcttca tgcaaccatg ctgcgtcttt atcacccgat ctccggcatc gaaatggaat
    23401 ggcatgcgcc tattccacaa gatatggtgg agctgattga ggtgatgcgc gccgatttcg
    23461 aagagcataa ggatgaagtg gactggttat gagtaagctg attgtcccgc agtggccgct
    23521 accaaaaggt gttgcggcct gtagctcttc tcgtatcggc ggcgtgagct tgcccccgta
    23581 tgactcgctc aacctcggtg cccattgtgg cgataacccg gatcacgttg aggagaatcg
    23641 caagcgactt tttgctgcgg gcaatttgcc ttctaaaccg gtctggcttg agcaggtaca
    23701 cggcaaagat gtgcttaagc ttactggcga accttatgcc tcaaaacgag cagatgcctc
    23761 ttatagcaat acgcccggca cggtttgcgc agtgatgact gccgactgcc tccctgtgct
    23821 gttttgcaat cgagcgggaa cggaagtcgc cgccgctcat gctggctggc gtggactgtg
    23881 cgcaggcgtg ctggaagaga cggtttcctg ttttgctgat aatccggaaa atattctcgc
    23941 ctggttaggg ccggcaattg gtccacgcgc gttcgaagtg ggggtggagg ttcgcgaggc
    24001 gtttatggca gtagacgcta aagcaagtgc agctttcatt cagcatggtg ataagtatct
    24061 ggcggatatt tatcagcttg cccggcagcg tctggcgaac gtgggtgttg agcaaatttt
    24121 cggcggcgac cgttgtacat atacggaaaa tgagactttc ttctcttatc gtcgcgacaa
    24181 gaccaccggt cgtatggcaa gtttcatttg gctgatataa cctaaagaat caagacgatc
    24241 cggtacgcgt gattttcttt tcacattaat ctggtcaata accttgaata attgagggat
    24301 gacctcattt aatctccagt agcaactttg atccgttatg ggaggagtta tgcgtctgga
    24361 tcgtcttact aataaattcc agcttgctct tgccgatgcc caatcacttg cactcgggca
    24421 cgacaaccaa tttatcgaac cacttcattt aatgagcgcc ctgctgaatc aggaaggggg
    24481 ttcggttagt cctttattaa catccgctgg cataaatgct ggccagttgc gcacagatat
    24541 caatcaggca ttaaatcgtt taccgcaggt tgaaggtact ggtggtgatg tccagccatc
    24601 acaggatctg gtgcgcgttc ttaatctttg cgacaagctg gcgcaaaaac gtggtgataa
    24661 ctttatctcg tcagaactgt tcgttctggc ggcacttgag tctcgcggca cgctggccga
    24721 catcctgaaa gcagcagggg cgaccaccgc caacattact caagcgattg aacaaatgcg
    24781 tggaggtgaa agcgtgaacg atcaaggtgc tgaagaccaa cgtcaggctt tgaaaaaata
    24841 taccatcgac cttaccgaac gagccgaaca gggcaaactc gatccggtga ttggtcgtga
    24901 tgaagaaatt cgccgtacca ttcaggtgct gcaacgtcgt actaaaaata acccggtact
    24961 gattggtgaa cccggcgtcg gtaaaactgc catcgttgaa ggtctggcgc agcgtattat
    25021 caacggcgaa gtgccggaag gattgaaagg ccgccgcgta ctggcgctgg atatgggcgc
    25081 gctggtggct ggggcgaaat atcgcggtga gtttgaagaa cgtttaaaag gcgtgcttaa
    25141 cgatcttgcc aaacaggaag gcaacgtcat cctgtttatc gacgaattac ataccatggt
    25201 cggcgcgggt aaagccgatg gcgcaatgga cgccggaaac atgctgaaac cggcgctggc
    25261 gcgtggtgaa ttgcactgcg taggtgccac gacgcttgac gaatatcgcc agtacatcga
    25321 aaaagatgct gcgctggaac gtcgtttcca gaaagtgttt gttgccgagc cttctgttga
    25381 agataccatt gcgattctgc gtggcctgaa agaacgttac gaattgcacc accatgtgca
    25441 aattactgac ccggcaattg ttgcagcggc gacgttgtct catcgctaca ttgctgaccg
    25501 tcagctgccg gataaagcca tcgacctgat cgatgaagca gcatccagca ttcgtatgca
    25561 gattgactca aaaccagaag aactcgaccg actcgatcgt cgtatcatcc agctcaaact
    25621 ggaacaacag gcgttaatga aagagtctga tgaagccagt aaaaaacgcc tggatatgct
    25681 caacgaagaa ctgagcgaca aagaacgtca gtactccgag ttagaagaag agtggaaagc
    25741 agagaaggca tcgctttctg gtacgcagac cattaaagcg gaactggaac aggcgaaaat
    25801 cgctattgaa caggctcgcc gtgtggggga cctggcgcgg atgtctgaac tgcaatacgg
    25861 caaaatcccg gaactggaaa agcaactgga agccgcaacg cagctcgaag gcaaaactat
    25921 gcgtctgttg cgtaataaag tgaccgacgc cgaaattgct gaagtgctgg cgcgttggac
    25981 ggggattccg gtttctcgca tgatggaaag cgagcgcgaa aaactgctgc gtatggagca
    26041 agaactgcac catcgcgtca ttggtcagaa cgaagcggtt gatgcggtat ctaacgctat
    26101 tcgccgtagc cgtgcggggt tggcggatcc aaatcgcccg attggttcat tcctgttcct
    26161 cggcccaacg ggtgtgggga aaactgagct ttgtaaggcg ctggcgaact ttatgtttga
    26221 tagcgacgag gcgatggtcc gtatcgatat gtccgagttt atggagaaac actcggtgtc
    26281 tcgtctggtt ggtgcgcctc cgggatatgt cggttatgaa gaaggtggct acctgaccga
    26341 agctgtgcgt cgtcgtccgt attccgtcat cctgctggat gaagtggaaa aagcgcatcc
    26401 ggatgtcttc aacattctgt tgcaggtact ggatgatggt cgtctgactg acgggcaggg
    26461 gagaacggtc gacttccgta atacggttgt cattatgacc tctaacctcg gttccgattt
    26521 gattcaggaa cgcttcggtg aactggatta tgcgcacatg aaagagctgg tgctcggtgt
    26581 ggtaagccat aacttccgtc cggaattcat taaccgtatt gatgaagtgg tggtcttcca
    26641 tccgctgggt gaacagcaca ttgcctcgat tgcgcagatt cagttgaaac gtctgtacaa
    26701 acgtctggaa gaacgtggtt atgaaatcca catttctgac gaggcgctga aactgctgag
    26761 cgagaacggt tacgatccag tctatggtgc acgtcctttg aaacgtgcta tccagcagca
    26821 gatcgaaaac ccgctggcac agcaaatact gtctggtgaa ttggttccgg gtaaagtgat
    26881 tcgcctggaa gttaatgaag accggattgt cgccgtccag taaatgataa aacgagccct
    26941 tcggggctcg tttttgtcta taagttagac ggaaaagact atatttaaga tgttttgcct
    27001 gaaaaatgag cgaacgataa agtttttata tttttcgctt gtcaggccgg aataactccc
    27061 tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc
    27121 tcctgagaat tccggcagag aaagcaaaaa taaatgcttg actctgtagc gggaaag
//
LOCUS       DAZFFI010000060        26981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFFI010000060 DAZFFI010000000
VERSION     DAZFFI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.060"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(219..866)
                     /gene="ychE"
                     /locus_tag="ACHJ12_002925"
     CDS             complement(219..866)
                     /gene="ychE"
                     /locus_tag="ACHJ12_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4990685.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(897..950)
                     /gene="ychE"
                     /locus_tag="ACHJ12_002926"
     CDS             complement(897..950)
                     /gene="ychE"
                     /locus_tag="ACHJ12_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4990686.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1343..4018
                     /gene="adhE"
                     /locus_tag="ACHJ12_002927"
                     /gene_synonym="adhC"
     CDS             1343..4018
                     /gene="adhE"
                     /locus_tag="ACHJ12_002927"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4990687.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4142..5042
                     /locus_tag="ACHJ12_002928"
                     /pseudo
     CDS             4142..5042
                     /locus_tag="ACHJ12_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5324..5941)
                     /gene="tdk"
                     /locus_tag="ACHJ12_002929"
     CDS             complement(5324..5941)
                     /gene="tdk"
                     /locus_tag="ACHJ12_002929"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4990688.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6546..6959
                     /gene="hns"
                     /locus_tag="ACHJ12_002930"
     CDS             6546..6959
                     /gene="hns"
                     /locus_tag="ACHJ12_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4990689.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7103..8011)
                     /gene="galU"
                     /locus_tag="ACHJ12_002931"
     CDS             complement(7103..8011)
                     /gene="galU"
                     /locus_tag="ACHJ12_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4990690.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8213..9226)
                     /gene="rssB"
                     /locus_tag="ACHJ12_002932"
     CDS             complement(8213..9226)
                     /gene="rssB"
                     /locus_tag="ACHJ12_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4990691.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9318..10223)
                     /gene="rssA"
                     /locus_tag="ACHJ12_002933"
     CDS             complement(9318..10223)
                     /gene="rssA"
                     /locus_tag="ACHJ12_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4990692.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10336..10794
                     /gene="ychJ"
                     /locus_tag="ACHJ12_002934"
     CDS             10336..10794
                     /gene="ychJ"
                     /locus_tag="ACHJ12_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4990693.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10844..11686
                     /gene="purU"
                     /locus_tag="ACHJ12_002935"
     CDS             10844..11686
                     /gene="purU"
                     /locus_tag="ACHJ12_002935"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4990694.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11846..11930
                     /locus_tag="ACHJ12_002936"
     tRNA            11846..11930
                     /locus_tag="ACHJ12_002936"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11880..11882,aa:Tyr,seq:gta)
     gene            11978..12109
                     /locus_tag="ACHJ12_002937"
     ncRNA           11978..12109
                     /ncRNA_class="other"
                     /locus_tag="ACHJ12_002937"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12156..12287
                     /locus_tag="ACHJ12_002938"
     ncRNA           12156..12287
                     /ncRNA_class="other"
                     /locus_tag="ACHJ12_002938"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12471..13148)
                     /gene="narI"
                     /locus_tag="ACHJ12_002939"
     CDS             complement(12471..13148)
                     /gene="narI"
                     /locus_tag="ACHJ12_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4990695.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13148..13858)
                     /gene="narJ"
                     /locus_tag="ACHJ12_002940"
     CDS             complement(13148..13858)
                     /gene="narJ"
                     /locus_tag="ACHJ12_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4990696.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13855..15393)
                     /gene="narH"
                     /locus_tag="ACHJ12_002941"
     CDS             complement(13855..15393)
                     /gene="narH"
                     /locus_tag="ACHJ12_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4990697.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQLTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15390..19133)
                     /gene="narG"
                     /locus_tag="ACHJ12_002942"
     CDS             complement(15390..19133)
                     /gene="narG"
                     /locus_tag="ACHJ12_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4990698.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(19648..21039)
                     /gene="narK"
                     /locus_tag="ACHJ12_002943"
     CDS             complement(19648..21039)
                     /gene="narK"
                     /locus_tag="ACHJ12_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4990699.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            21378..23174
                     /gene="narX"
                     /locus_tag="ACHJ12_002944"
     CDS             21378..23174
                     /gene="narX"
                     /locus_tag="ACHJ12_002944"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4990700.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            23167..23817
                     /gene="narL"
                     /locus_tag="ACHJ12_002945"
     CDS             23167..23817
                     /gene="narL"
                     /locus_tag="ACHJ12_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4990701.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(23818..25212)
                     /gene="ychO"
                     /locus_tag="ACHJ12_002946"
                     /gene_synonym="iatA"
     CDS             complement(23818..25212)
                     /gene="ychO"
                     /locus_tag="ACHJ12_002946"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4990702.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            25397..25750
                     /gene="ychN"
                     /locus_tag="ACHJ12_002947"
     CDS             25397..25750
                     /gene="ychN"
                     /locus_tag="ACHJ12_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4990703.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25794..26489)
                     /gene="chaC"
                     /locus_tag="ACHJ12_002948"
     CDS             complement(25794..26489)
                     /gene="chaC"
                     /locus_tag="ACHJ12_002948"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4990704.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(26647..26877)
                     /gene="chaB"
                     /locus_tag="ACHJ12_002949"
     CDS             complement(26647..26877)
                     /gene="chaB"
                     /locus_tag="ACHJ12_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4990705.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT         6877 a         7057 c         6511 g         6536 t
ORIGIN      
        1 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
       61 gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
      121 tttacctatt ccttttgctt ttgataactg cgcgggaaca aagtaaaaat aatgacgaag
      181 taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag gccggggaaa
      241 atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa tagccccata
      301 atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca cggtgccatg
      361 cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa accaaacaga
      421 tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc ccctggcccc
      481 gccatcaacg gcaacgccaa tggcaccaca ccaatgcttt cacgtaccgc ggtttctgat
      541 ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga catcgctatc
      601 gtcaccacca ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc aaaaagttgc
      661 agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga caggttggct
      721 gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct gataaagacg
      781 ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt gaagtaaacg
      841 ggaaaatcaa aaaaggtctg aatcacggtt agctccgaag caaaagccgg ataatgttag
      901 ccataaataa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat ttttcacctc
      961 ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa acatgctaat
     1021 gtagccacca aatcatacta caatttatta actgttagct ataatggcga aaagcgatgc
     1081 tgaaaggtgt cagctttgca aaaatttgat ttggatcacg taatcagtac ccagaagtga
     1141 gtaatcttgc ttacgccacc tggaagtgac gcattagaga taataactct aatgtttaaa
     1201 ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt cataggttaa
     1261 gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta ctaaaaaagt
     1321 ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta acgcactcgt
     1381 agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc aagtagacaa
     1441 aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg cgaaaatggc
     1501 cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc actttgcttc
     1561 tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt ctgaagacga
     1621 cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta tcgttccgac
     1681 cactaacccg acttcaactg ctatcttcaa atcgctgatc agtctgaaga cccgtaacgc
     1741 cattatcttc tccccgcacc cgcgtgcaaa agatgccacc aacaaagcgg ctgatatcgt
     1801 tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga tcgatcaacc
     1861 ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga tcctcgcgac
     1921 tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta tcggtgtagg
     1981 cgcgggcaac actccagttg ttatcgatga aactgctgat atcaaacgtg cagttgcatc
     2041 tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt
     2101 tgttgttgac tctgtttatg acgctgtacg tgaacgtttt gcaacccacg gcggctatct
     2161 gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa
     2221 cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct tctctgtacc
     2281 agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc
     2341 acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg aagacgcggt
     2401 agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt gcctgtacac
     2461 tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga aaacggctcg
     2521 tatcctgatt aacaccccag cgtctcaggg tggtatcggt gacctgtata acttcaaact
     2581 cgcaccttcc ctgactctgg gttgtggttc ttggggtggt aactccatct ctgaaaacgt
     2641 tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa acatgttgtg
     2701 gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg cgctggatga
     2761 agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa
     2821 tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt
     2881 cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag aactggcaaa
     2941 ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa
     3001 gatcatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg cgctgcgctt
     3061 tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat
     3121 cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga
     3181 cgacgctact ggtcagaaat atccgctggc agactatgcg ctgactccgg atatggcgat
     3241 tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg gtggtctgga
     3301 cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt tctctgatgg
     3361 tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct accacgaagg
     3421 gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcgactatcg cgggtatcgc
     3481 gtttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt
     3541 ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc gctacaatgc
     3601 gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc aggctcgccg
     3661 tcgttatgct gaaattgccg accacctggg tctgagcgca ccgggcgacc gtactgctgc
     3721 taagatcgag aaactgctgg catggctgga aacgctgaaa gctgaactgg gtattccgaa
     3781 atctatccgt gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc
     3841 tgaagatgca ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc tgatctccga
     3901 gctgaaacag attctgctgg atacctacta cggtcgtgat tatgtagaag gtgaaactgc
     3961 agcgaagaaa gaagctgctc cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaatc
     4021 agtagcgctg tctggcaaca taaacggccc cttctgggca atgccgatca gttaaggatt
     4081 agttgaccga tccttaaact gaggcactat aacggcttcc acaacaggga gccgttttct
     4141 tatgccactt ctcaatgatc tgctcgattt cagtgaccat ccgcttatgc ctccgccctc
     4201 tgcacaacta tttgcagaac accttcccac cgagtggata caacactgcc tgacgctttc
     4261 tgctcatgcg accgttcgcc gccgtcgttt accgggggac atggttatct ggatggtggt
     4321 gcaatgagcc aattaccgat gttgttcgcc gtctgaacct gagcgcggat ggcgaagcgg
     4381 ggatgaacct gctggcccgc agcgctgtca cccaggcgcg tcagcgcgtg ggggccgccc
     4441 cagtggaatg gctcttccgc cagaccgcac agacaggggc gcggaacgtt acctgaagga
     4501 tgactggcac ggcctgcaac tttttgccat tgatggcgca cagttcagga cacctgactg
     4561 ataaacccga gctgcgtgaa tattatgggt ctgccaacac atccactaag cggcagaacg
     4621 cctacccggt tatgcgtctg gtggccttaa tgaacctggg aagtcacatt ctgctgaatg
     4681 ccgtgaccgc accttaccgg cagagcgaaa ctgtgctggc ccactccatg ctcgccacca
     4741 tcccggataa ctctattacg ttgtttgata aactttttta cagcgaagac ctgctgctga
     4801 cgctgaacca gaagggttgt aaccgccact ggctactgcc tgcgtggaag aatatcgcat
     4861 cagaaatgat agagctggga aacacggcgt caccagggac catcccgaaa cgactggagc
     4921 atcttcaggg tgctctggaa gtggtgttca taacaaaacg cccccgacca tcaaggccga
     4981 ggtcggttaa gatatcaaaa acccgttatc ctgtgaaaca tagtgccgct cctcttaagt
     5041 gaacggcatt gcccttctgg ggccgtattt tttgtttacc caaagcaact tttccataaa
     5101 ccgacagcat tagccttcat catatttgcg acgatgtata acgcctaaac acagggatat
     5161 tgtactttac aggtcacaag tcaacgtcgg tgcttaagag ccctgtgagg cgtatagcgg
     5221 cgttaaaaaa ctgccgagaa gggtatatag cccggaagaa gtgcgtaaaa cgaactgaca
     5281 ggataaaagt gccctgctca ccctgtcagt aaagaaattc ttattaatcg tggcgatgcc
     5341 tttcctgaat agccgttaat gagccgactt gtaacgcctc tttatagtgt ttacggcata
     5401 cagaaacgta tcgttcatta ccaccaatta ccacctgctc accttcgtta taaggtctgc
     5461 ctgcttgatc aagacgcagc accatgcttg ctttacggcc acaaaaacag atggttttta
     5521 attcaaccag tttgtcggac catgccagta agtattggct gccaataaat aattcacctc
     5581 gaaaatcggt acgtaaacca taacaaagta cgggtatatc gagttgatcg acaacctccg
     5641 ataattcata tacttgttgt ctggttaaaa actggcattc atcaaccagt acgcaatgaa
     5701 ttgcctgctg ttcatgttcc gcacgaatct catcaaataa tgatgaattt tggttaaata
     5761 attttgcagg cgatgacaaa cctatacgcg aactgacttt cccggcacca aagcgatcat
     5821 caatttctgc cgtatatacg acagtgcgca tgccgcgttc ctggtaattg tatgaagatt
     5881 gcaacaatgc tgtagactta cccgcattca ttgcggaata gtagaaatat agctgtgcca
     5941 tcagccacag gccctcaatg atatgaaata aagttggctg gagtttatca taattcgtca
     6001 gtttttcagc tcaacagtat gcacagaagt gggtatatgc gttctccctt acgaagcctt
     6061 gcataatcct tctgagctat cattacaact gcctcgcttg ttataagcgg gtaaatgact
     6121 gctggtaact attcacaatc tttaacctgt tgcgcaagta atagccctct gttgacctcc
     6181 aggagatagt gcaatactaa gtccatgctc ttattgcgac tgttctactt ttcatcattc
     6241 gcttaatagg gaattctcgt aaacacaact aatacagaag actgaaaggt cgtcagccta
     6301 cgataatctc cccataaaat gtgacatgaa tcaggaagtt ttaacctcac gtgctgcgaa
     6361 atcatcggtg taaatagggc tatatgccgc gtcttttctg gctaatttta tgaaaagata
     6421 tttattggcg gcacaaaata aagaacaatt ttgaattcct tacattcctg gctattgcac
     6481 aactgaattt aaggctctat tattacctca acaaaccacc ccaatataag tttgagatta
     6541 ctacaatgag cgaagcactt aaaattctga acaacatccg tactcttcgt gcgcaggcaa
     6601 gagaatgtac acttgaaacg ctggaagaaa tgctggaaaa attagaagtt gttgttaacg
     6661 aacgtcgcga agaagaaagc gcggctgctg ctgaagttga agagcgcact cgtaagctgc
     6721 agcaatatcg cgaaatgctg atcgctgacg gtattgaccc gaacgaactg ctgaatagcc
     6781 ttgctgccgt taaatctggc accaaagcta aacgtgctca gcgtccggca aaatatagct
     6841 acgttgacga aaacggcgaa actaaaacct ggactggcca aggccgtact ccagctgtaa
     6901 tcaaaaaagc aatggatgag caaggtaaat ccctcgacga tttcctgatc aagcaataat
     6961 cttttgtaga ttgcacttgc ttaaaatccc gccagcggcg ggatttttta ttgtccggtt
     7021 taagacaatt taataagaat aagctataaa aaaacggcgt cgattgctca acgccgtttc
     7081 gtggataaca ccgatacgga tgttacttct taatgcccat ctcttcttca agccaggctt
     7141 taaattccgt gccaagggtg ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac
     7201 ctaatttatt accgcagtca tggctcttcc ctttcatatg ataggcttcc accgtttctt
     7261 tttcgatcag catatcaatt gcgtcggtga gctgaatttc atcaccagct cccggagggg
     7321 tttttgccag caacggccaa atatccgcgc taagtacgta acgacccaca atagcgagat
     7381 tagacggcgc aacatccgct ttcggttttt ctaccacacc aaccatcggt acgctttcac
     7441 ccggcgctaa ttcaacgcct ttgcaatcca caacgccata tgcggtcaca tcagcaaccg
     7501 gttcaaccat gatctggcta tgacccgttt catcaaagcg gcggatcatc tctgccaggt
     7561 tatcctgtga caaatcggat tcatattcat ccagaataac atcaggcaaa ataacagcta
     7621 ccggttcatc acccactacc gggtgagcac acaataccgc gtgtcccagg cctttcgcca
     7681 gaccctgacg aacttgcata atagtcacgt gcggtggaca aatagactgc acttcatcaa
     7741 gcagttgacg ttttacacgt ttttccagca ttgcttccag ttcaaaactg gtatcaaagt
     7801 ggttttcaat agagttttta gatgagtgtg taaccagcac aatttcagta atgccagccg
     7861 caatacattc attcacgacg tattgaatta atggcttatc gacaagtggc agcatctctt
     7921 tcgggatggc tttcgtcgcc ggcaacatcc tggttcctaa tcccgcaacg gggataacgg
     7981 cttttttgac tttcgtatta atggcagcca tttaaattct cctggactgt tcgtgttttg
     8041 aacgtgttca tatttctgta tcgcatccca gtatatcagc accccgaagg ggctaagttc
     8101 tgaaaaggag gcgagttagc ataattaaga caattactta tacgaatggc gacgatagta
     8161 ccaccagccg acattagcag gtaatgcaaa tttagcccgc gttatcgttt gctcattctg
     8221 cagacaacat caagcgcagt cgaccaccgg ttccccatat ttggcattgc caggcatcgc
     8281 atcgctggct caattgattc aaataagcgt tacctaaagt gcctaacgga acaccattac
     8341 tgatttgcac ctggtgttcg ccggtattta acgtcgcatt cagacccgca gaaaccagaa
     8401 tgagattttt cagttcgcga tgataatagc caactaatag cggaaactgc cccggcagat
     8461 tggcctgacg aagtaaatgg tttacctgct tcaataacgc gcccaactct ggcaaccgtt
     8521 gattttggtg tgcaagctgt tcctgtaata atccgttaaa caatgcgcgt aataacaagg
     8581 cagcaagtac gccattatgt ccagctcggg tgacatcaag gcaataaaat gccagatcgt
     8641 tttccgaaag tgcggcaata tcaagcacca ggccgggttt gtccgcggca accaattgac
     8701 gataattaac ccggcaatgg gaaatcacct gctgaaccgg cggttgtagt tcctgtaata
     8761 atttcgccgc tgcggcaggg ttatcaacca ttgcatccca gtcgcgaaaa agcctttcct
     8821 cttcctcaac gcgcgaatta aacatgctgg gatagagaca ggcaaaaacc atctcgcgca
     8881 agcgattcag atctttaact ggtttcagca aaacatcttc aacgcccaga cgtaacgctt
     8941 tggcaatatc tgccatattt tcggtggcag atatcaccag aaccggtgtc tggtcacctc
     9001 tgttgcgtat atgttccagc agtttaagcc cgttcattcg tggcatcgcg atatcacata
     9061 tcatcaggtc tggagtgaaa cctcccagca actcaagggc atccacccca tcagccgcca
     9121 gtaccgttgt cgctcccaat gaggaaaacc acgaatccag aagcgagcga aataccggct
     9181 catcttcaac aatgagaatc tgttttccga ccaatggctg cgtcatgttc tctcccctga
     9241 ctggctttac tcaatagtgg catgctattg ccacctgcgc ctgtcagaat taacttaagt
     9301 gtagataaaa attctggtca aatgttggtg cgtaccaacg gcaaaagttc gtccattttc
     9361 ttttccactg ccagctgtcc ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta
     9421 gatatttgcg ggcaaacagg ttgaatcaga atatcgggcg gatcacctgc catgcggttc
     9481 cttttaaggc ggttctccag cacctggata gaagtggtca taatctctgt tgccgttggc
     9541 gctgtcaccg cccgacgtgt cgttatgctg cctaaccttt ctttcagacg cgcatgccac
     9601 ggcagagaat caccattctc gctattttct tcactgacat taaaggagag caaatcttgt
     9661 tgcatcaaat gagcatcgtg ctgcaggtca accgctatca caatatcagc ccccaatgca
     9721 cgcgtgaggg aaataggaat tgggttaacg actgctccat caaccagcca gtagccgtta
     9781 tgtgcaacag gtgccatgag tcctggaata ctgcatgatg cgcgaatagc aagatggaga
     9841 tcgccttcag taaaccataa ttcacgtccc gtacttaaat tggtggcaac agccgcaaag
     9901 cgacgggaac aattttcgat ctctgtttcc ggcattattt cgcggtattg attgaagaca
     9961 cgctcgccgc gcagtaaccc accgcgctgc caggagagat ccatcaggcg taaaacatcc
    10021 caataactga aagaggtcac ccaatcttcc agcgcagata atcgatcgca tgcataggca
    10081 gcgcccacca gcgaaccaat tgaacatcct gcaacgatat caatttcaat acccactttt
    10141 tttagcgcat taataacgcc aatatgcgac caacctctcg ccgcgccaga tcccagcgcc
    10201 agccctattt ttatctttct cattattccc gcaagattcc cctggaatgc aatcgtagcc
    10261 acatcgctac tgctcggtta acatagtgct actcaggcgg ttaatacgcc gttattgttt
    10321 ccagggagat tatttgtgtc tcagctttgt ccctgtggta gtgctgtcga gtatagccta
    10381 tgttgccacc cttatgtgtc tggtgaaaag gttgcacctg atccagaaca tctcatgcgt
    10441 tcgcgttact gcgcttttgt gatgcaagac gcagattatt taataaagac ctggcatccc
    10501 tcttgtggag cagcggcgtt acgtgccgaa ttgatggccg gatttgcgca tactgaatgg
    10561 ttgggattga ccgtatttga acattgttgg caggacgccg acaatatcgg atttgtcagt
    10621 tttgttgctc gatttaccga aggtggtaaa acaggtgcaa taattgaacg ttcgcgattt
    10681 ttgaaagaaa acggtcaatg gtactatatt gacggtacac gtccgcagtt tggtcgcaac
    10741 gatccctgcc cttgtggttc aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg
    10801 acggtacggt ttagcaaaca ctctcaacaa ggtttttcca gcaatgcatt cactccaacg
    10861 taaagttctg cgtactattt gtccggacca aaaaggtctg atcgcacgta ttaccaatat
    10921 ttgctacaag cacgagttaa atatcgtaca gaacaatgaa tttgttgatc accgtaccgg
    10981 gcgctttttt atgcgcacgg aactggaagg gatttttaat gattccaccc tgctggcgga
    11041 tctcgatagc gcattgccag aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg
    11101 gatagtgatt ctggtcacta aagaagcgca ttgccttggc gatttgttga tgaaagccaa
    11161 ttacggcggc ctggatgtcg aaatcgcggc agttattggt aaccacgata ctttacgttc
    11221 tctggttgag cgttttgata ttccgtttga gctggtaagc catgaagggt taagccgcaa
    11281 cgagcacgat caaaagatgg cggacgccat tgatgcttat caacctgact atgtggtgct
    11341 ggcgaagtat atgcgggtat taacgccgga atttgtggca cgcttcccga ataaaatcat
    11401 caatattcac cattcattcc tgcctgcgtt tatcggcgca cgtccttatc accaggccta
    11461 tgaacgtggt gtgaagatta ttggcgcaac cgctcactat gtgaatgaca atctggacga
    11521 aggcccaatc atcatgcagg acgttattca tgtcgatcat acctacacag ctgaagatat
    11581 gatgcgcgca ggtcgtgacg tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact
    11641 ggcacagcgc gtctttgttt acggtaatcg gacgattatt ctttaatcgc cagcaaaaat
    11701 aactggttac ctttaatccg ttacggatga aaattacgca accagttcat ttttctcaac
    11761 gtaacacttt acagcggcgc gtcatttgat atgatgcgcc ccgcttcccg ataagggagc
    11821 aggccagtaa aaagcattac cccgtggtgg ggttcccgag cggccaaagg gagcagactg
    11881 taaatctgcc gtcatcgact tcgaaggttc gaatccttcc cccaccacca tcacttttca
    11941 aaagtccgta aactctcaaa cgaatccgca attaaatatt ctgcccaagc ggggaaggat
    12001 gagaagcttc gaccaaggtt cgacttgagc gccagcgaga gagcgttgcc gcaggcaacg
    12061 acccgaaggg cgaagcgcgc agcgctgagt aatccttccc ccaccaccat cactttcaaa
    12121 agtccgtgaa ctctcaaacg aatccgcgat taaatattct gcccatgcgg ggaaggatga
    12181 gaagcctcga ccaaggttcg actcgagcgc cagcgagaga gcgttgccgc aggcaacgac
    12241 ccgaagggcg aagcgcgcag cgttgagtaa tccttccccc accaccatca cttccttaaa
    12301 taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt accgggccta
    12361 caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac cccgcaccgt
    12421 gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc ttagtgacga
    12481 gcgcgcacta gctggtactt acgtgtcaga tactccaccg gtacgctcca gatgtgtacc
    12541 agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg cagacggaag
    12601 ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac caccgactgc
    12661 gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga gaacggaatg
    12721 gtcaacaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat atccgctccg
    12781 gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag cacgccgcca
    12841 atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg tttcacttca
    12901 atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa gtgaccaaca
    12961 aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc tttgcgatcc
    13021 agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg cagccagcta
    13081 ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa catattcagg
    13141 aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg acggcaccgg
    13201 caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca gcaaagaatt
    13261 taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg cgcgcttcgt
    13321 cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat ttcagcagca
    13381 gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc agcaatgcca
    13441 gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc tgcgacaggt
    13501 actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc aagccgtgct
    13561 gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat tcaccgtgaa
    13621 catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg ctgtactggg
    13681 cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc agcgcatggg
    13741 catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct tgttgatgct
    13801 gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt tcgatcatgg
    13861 atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt tgaacagatt
    13921 gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc catttttctc
    13981 cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc gatcttcgta
    14041 gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca gaccgacctc
    14101 ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt aatgacgcat
    14161 cgccagcata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg tcagcagatt
    14221 cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaatgc cgttgctacc
    14281 cagctcaccc gcgtctgccg cagactgaat cggagacaga ggcggcacgt accagaccat
    14341 cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca ttgccatttt
    14401 ataaaccggc gactgctgtg cggcttcaat aacgctcagc ggaataccgt ctttaatcgc
    14461 ctgctcgatg actttcggat cgttcggatc gaggaacacc tccagctgac gctggtaaag
    14521 atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt acagcagcac
    14581 gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcagct gacccgcttc
    14641 aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt tgaagtagat
    14701 ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat cctggtcgat
    14761 caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca
    14821 tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg tgttttcgaa
    14881 ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca gtttgtcaaa
    14941 ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca tccgttcccc
    15001 ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg gcgcggtatg
    15061 caggttctga tagtcaaaat cgaacggctc gtaataatcg tcgatccccg gcagatgcgg
    15121 gttagcgaag attttaccca gcaacatggc acggttaccc atgcgcggct gcagtttgcc
    15181 gttgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt cagtcgggaa
    15241 gccctgaccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc cttcacggct
    15301 ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact tatcgagatt
    15361 cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac ctggtcattg
    15421 ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg
    15481 ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata gccgccgatc
    15541 atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc acgctgttgg
    15601 gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat catggtcatc
    15661 cctgccggaa cacgctggct gacaacagca cgggcagtca gagcaccgtt gctgttgaat
    15721 acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc actcaaccag
    15781 accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta ggtggagtgg
    15841 ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt gccgttggat
    15901 ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata aaccagcagg
    15961 ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg accagagagc
    16021 gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca
    16081 tcttccagac cagaccaggt cgggctggag ataattttgc gcggctgtgc ctgaatatcg
    16141 cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc acgaccggta
    16201 aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc cggtgccagt
    16261 gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc tttcgccgga
    16321 ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg ggtgttccag
    16381 gcaatccctt taccgccatt accgattttc tccatcagcg ggccgataga ggtaaagcgt
    16441 tcgtaagtcg ccggataatc gcgctctacg accataatgt gtggcgcggt tttaccaggg
    16501 atcaggtcgc actcgccttt tttccagtct ttcacatcca gcggctgcgc cagttcagcg
    16561 gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag atggccgacg
    16621 cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct tttcgcttcc
    16681 caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc cgaagtattc
    16741 atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg
    16801 ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag accattgtcc
    16861 tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt accctggata
    16921 ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc ggaagaaccg
    16981 agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt ttctggttgt
    17041 tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc caccggattc
    17101 atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa ctgcggtgca
    17161 gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg tccggtatag
    17221 cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc ataacgccac
    17281 tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg acgctgccag
    17341 tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc ctgacctaca
    17401 tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag catgttgatc
    17461 agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc gacgataatc
    17521 atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat gcgaataatc
    17581 tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc tttcacatcg
    17641 tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta gtttgccagc
    17701 gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg caggcgtttc
    17761 accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg ttccgtgccg
    17821 tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc cagcaggctc
    17881 agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt ccatttgccc
    17941 ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc attggtatta
    18001 aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac cagatcagca
    18061 gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat caccagcatc
    18121 ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt gtcgaggtgg
    18181 aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt gccctgtttc
    18241 ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt gacggcaaca
    18301 gttttggtcc ctttgtaacg cacttcagta aagaagtgag catccggggt acgcgtctgc
    18361 ggcacgtttg acccccaggc gatgatgtag ctggagttgt accagtcagc agattccggt
    18421 acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaagtcgca gtaccagtcg
    18481 tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga tgcgtaagaa
    18541 accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc gtagtttttg
    18601 atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccagga agaacgaaca
    18661 aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc ttcaatgata
    18721 gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca cattttcatc
    18781 aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata ccaggagtag
    18841 ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg ggtacgcgga
    18901 tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat tttccagctg
    18961 caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg ctgccaacgc
    19021 tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg cccatcggca
    19081 aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact catcggtttt
    19141 ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt aagggcaagg
    19201 taacgctctg aaacagcgtg ggaattgata acgatcaagt gtgatgggga tgaaaaataa
    19261 agtaaattcc taagataccc ctttaggata attaacagtt aaagcataac atgctgtaaa
    19321 ttaagacgtt ttatacctgt agttatttaa agcatgaaga tttttatgca gatgggtatt
    19381 aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg cgaataagcc
    19441 atataacatt agtatgttgt aactaaataa cgcataaaaa aagcgcgaca tcatgccgcg
    19501 caaaggataa tcaaatattt tactctatat aattcggttt acaggaaggc ggcaagtgaa
    19561 tgaatcccag gagcttacta aagtaagtga ctggggtgaa tgaacgcagc cgcagcacat
    19621 gtaacccgaa gtatgacgag taacggttta ttttttagaa tgccgaccat ataccgccca
    19681 ggtaatcact acgcaggcga tatagaaaat caaaaatacc ttcattgcgc cgactggcga
    19741 acccgttaat gccagcgagc taccaaacgc tttcgggata aagaagccac caatagcgcc
    19801 aatcgcagag atgaaaccca gcgccgctgc cgtgtcggtt gccgcttcac gcatcgcacg
    19861 ttcgtcagaa cccccttctg ctttcacgcg atccattgtc agtttacgga agatcactga
    19921 aatcatctgg aaagtggaac cactacccag cccagctgtc aggaacagcg ccaggaagac
    19981 cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc ggtaaggtca ggaacagcag
    20041 gccgctgaaa atcgccatca gaataaagtt caccagcgtg acacgagttc cgcccagacg
    20101 gtcagataat gcaccacctg cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc
    20161 gtattgcaga atctgaacat ccgggaactg cgtttttgac agcattgcaa agcccgcgga
    20221 gaagccgatg aaggagccga aggttgccag atacagcagg ctcataatcc acagatgacc
    20281 ccgtttgagt accggcaact gctccttgat ggaggctttc gaggtagcaa gatcgttcat
    20341 gccaaaccac gccgcaatag tgaagatggc aaggaacggc acccatatcc aggacgcatt
    20401 cgccagatac agctcagtcc catccggctg tttgacgccc tggctaccaa atactgcgaa
    20461 aatcgacagt gataccacca gcggagcaac caactgcatg acgctgacgc ccatgttgcc
    20521 cagaccacca ttcagaccca gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat
    20581 gtttgccata ctggaggcga agttcgcgcc agcaaagccg catagcagag agatgatgat
    20641 gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa cccagccaga cgcaaggaat
    20701 aatcagaata ccggtgctga acgccgtcca gcgacgacca ccgaagatag gaaccataaa
    20761 ggagtatgga acacgtaata acgcgccaga aaccgaaggc agcgcagtca acataaatag
    20821 ctgatcggtc gtaaaattaa agccgacttt cggtaggttc accgcaacag cgctgaacaa
    20881 catccatacg caaaacgcca gcagcagaca gggaacggaa atccacaggt tgcggctggc
    20941 aatacgttga ccgcgttgtt gccagaacgc aggatcttcc ggtcgccaat ctgtaatgac
    21001 agctccagta gccctttcgg gggcggatga gtgactcata gacacctctg atactcgttt
    21061 cgatgtctgc caccttagtg tctgtagcta aaggcaattt gatgtaaatc aaacgataag
    21121 ataactttat ctatcattga tatttatcat tacccatagt gagtacagtg atttcataaa
    21181 aattatgaga tttttcacgg tgctgtagaa tccctaccct taccgatgta aagcgactaa
    21241 ccacacggca aataaggagt aactctttcc aggtatgggt atacttcagc caatagccga
    21301 gaatactgcc attccagaat gtatcgtcac attcattaag gttattgctc atttaaagcc
    21361 tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc gctcaccctg gttaatcagg
    21421 ttgcgcttat tgtgttgctt tctactgcta ttggactggc agggatggcg gtttctggct
    21481 ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca
    21541 tgcaaagtta ccgtctgttg gcagcagtgc cattaagcga gaaagacaag cccttaatta
    21601 aagagatgga acaaacggca tttagcgccg agttgactcg agcagcagaa cgagacggac
    21661 aactggcgca attacagggt ttacaagatt actggcgtaa tgaactgatc cctgcgttga
    21721 tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag ccagtttgtt gccgggcttg
    21781 atcagctggt atctggtttt gaccgcacca cggaaatgcg catcgagaca gtggtactgg
    21841 tccatcgggt aatggcggta tttatggcac ttttactggt gttcactatt atctggttgc
    21901 gggcgcgact gctacaaccg tggcggcaac tgctggcaat ggcgagtgcc gtcagccatc
    21961 gcgattttac ccaacgcgca aacatcagcg ggcgcaacga aatggcgatg cttggaactg
    22021 cgttgaacaa tatgtctgca gaactggccg aaagttatgc cgtacttgag cagcgggttc
    22081 aggagaaaac cgccgggctg gagcataaaa atcagatcct ctcattttta tggcaggcta
    22141 accgccgttt gcattcccgc gccccgctgt gtgaacgcct gtcaccggta ctcaacggct
    22201 tacagaattt aaccctgcta cgtgatatcg aactgcgggt ttatgatact gatgatgaag
    22261 agaatcatca ggagtttacc tgccagccag atatgacttg tgatgataaa ggctgccagc
    22321 tctgcccgcg cggcgtatta cccgttggcg atcgcggcac aaccctgaag tggcggctgg
    22381 ctgactctca tacgcagtac ggtattttgc tggcgaccct gccgcagggg cgtcatctta
    22441 gccatgatca acaacaactg gtggataccc tggttgaaca actcaccgcc acgctggcgc
    22501 tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat ggaagagcgt gccaccattg
    22561 cgcgcgaact gcatgattct attgcccaat ctctctcttg tatgaagatg caggtgagtt
    22621 gtttacagat gcagggcgat gcgctgccag aaagcagccg cgaactgtta agtcagatcc
    22681 gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt gctcaccaca ttccgcttgc
    22741 agctcaccga gcctggatta cgtccggcgc tggaggcgag ttgcgaagag tacagcgcca
    22801 aatttggctt cccggtgaag ctggattatc aattgccgcc tcgtctggtg ccttcgcatc
    22861 aggcaatcca cttgttgcaa attgcccgtg aggcattaag taacgccctc aaacattcgc
    22921 aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa tcaggtcaaa ctgaccgtcc
    22981 aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag caatcactac ggcatgataa
    23041 taatgcgtga ccgtgcgcaa agtttacgag gcgattgccg cgtccgccgt cgtgaatcag
    23101 gtggcaccga agtggtggtc acctttattc ccgaaaaaac tttcacagac gtccaaggag
    23161 atacccatga gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg
    23221 cgaactggcg taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg
    23281 agtaatggcg aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta
    23341 gatctcaata tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc
    23401 ctctcagggc gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca
    23461 ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa
    23521 gcattgcatc aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg
    23581 gccgccagct tgcgtgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca
    23641 cgcgagcgcg atattctcaa gctgattgcc cagggtttgc cgaacaagat gattgcccgc
    23701 cgcctggaca tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg
    23761 aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat tttctgatta
    23821 cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac ggttcgacaa gcgttagcgt
    23881 gatctcattg gaggagacac gctgcccctg gttatcttcc accaccacag acaatcgcca
    23941 gtgattgctc gccctttccc cgttctgcca gtcaggcatg atcagcgtcc agccctccgc
    24001 gctgttggct tgtgcgcctg gcgttaaact taatatctgc gtatcgccct gccaaatcag
    24061 ttgccgaata ccgtaacgac tgcggatttg taatttcagt ggcactgttt cgccaggttt
    24121 tagatcccac ggaggtgtcg ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag
    24181 agtcggtaga ttatttcgct gcggattgtc atagcgacta ccacgtaacg actgactttc
    24241 ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca ccaaagcggt aattaagatt
    24301 cagcccgagg ttattttgat tctcgccact ttcaccctgt ttatgctggg ccgtcacagt
    24361 gactaatggc acaggggtgt aatttaatcc cagactcaac gcgacgggat tgtgataacc
    24421 cgtaccagag ttaaacaaat caacacgatc accaaaatac tgttctacgc tgacactggt
    24481 attgaggtgt tgatagaacg gcatgcgcat ccgggctgtc aggtcatacc cgcgcgccat
    24541 ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca aacggctgat aaaagtttgc
    24601 cgatagtcgc aaatattcgc cccacgcttc ggcaccaaag cccgctcgct gaagattttc
    24661 gtccagcaag ttgtcataaa aagtgttata acccaccagc cagttgccgc gcgcccagcg
    24721 ttgcccaacg cccacattgc tcaccaaccc atcatcctgc tgagtaagac caagctggct
    24781 ccaggtgaga taacgatcat tatcttgtaa cggcacaaac cagcttccac gactgccggt
    24841 gaaatgtcct tcgttatcca ctttgacgtc aacactggca tttccccacg gtgatagcca
    24901 ggactctacg tgctgattaa cctgttgact aagcgcgtcg cggacttttc ccaatgcgaa
    24961 agcttttgcc tgctcgccag tatccagccc gttatcattc atactggttt cgccgaaatc
    25021 tttcacaatt tcagcaaagt gtttttcccc atcatgattt tcgggagcca tgcctaaatc
    25081 cggcagacca tcattgttat tatcaaaggg atttgccgct ttttgctcga aggtagattg
    25141 tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa aacgggataa tgcgaggaac
    25201 gaaacggctc aaaacgtcgg taacttccgg taataatgaa aaaccatagc cttaaagaat
    25261 aacgattact ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg
    25321 tgaccgattg gtcagacgct gcagaagcag taagatagaa gatgaatgtc taattccctg
    25381 aatacaggag taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag
    25441 cgaatccttg tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct
    25501 ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc
    25561 aggggaaggc tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt
    25621 gaaattgtgc aaaacctgta ccgacgggcg cgggattagc acacttcctc ttattgatgg
    25681 ggtggaaatc ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct
    25741 cacattttaa tgtttgaggg ggcgtctgac gccccactat gagggaagtt tactcaggcg
    25801 aatcccggcc gtaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc
    25861 agcaaatcat tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa
    25921 aacaggtatt gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg
    25981 acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg
    26041 gcgtttactg tgcatccatc atcaagatca agctgacacc aggttggcag ataacagccg
    26101 gtaatcatct cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt
    26161 cgataggcga cgcctgtagt gcgtccgccc tctttcagtg caagcatccg tcccggctgg
    26221 tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt
    26281 gtaccggtgc acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg
    26341 aagatccaca ccggtccctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc
    26401 cgttgctctg ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg
    26461 gcattcatca agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc
    26521 gacaactttt tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac
    26581 aatatgcaat ccacggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta
    26641 ccggttttac gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac
    26701 agcagcccag gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcgtc
    26761 tttatcttta tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg
    26821 agacggtaga acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt
    26881 tgactccttt tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac
    26941 ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa a
//
LOCUS       DAZFFI010000061        26708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFFI010000061 DAZFFI010000000
VERSION     DAZFFI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.061"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..588
                     /locus_tag="ACHJ12_002950"
     CDS             <1..588
                     /locus_tag="ACHJ12_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4990706.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACHJ12_002951"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACHJ12_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4990707.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACHJ12_002952"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACHJ12_002952"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4990708.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACHJ12_002953"
     CDS             complement(1511..1978)
                     /locus_tag="ACHJ12_002953"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4990709.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHJ12_002954"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACHJ12_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4990710.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACHJ12_002955"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACHJ12_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4990711.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACHJ12_002956"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACHJ12_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4990712.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACHJ12_002957"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACHJ12_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4990713.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACHJ12_002958"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACHJ12_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4990714.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHJ12_002959"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACHJ12_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4990715.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACHJ12_002960"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACHJ12_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4990716.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHJ12_002961"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACHJ12_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4990717.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACHJ12_002962"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACHJ12_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4990718.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHJ12_002963"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACHJ12_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4990719.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHJ12_002964"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACHJ12_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4990720.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACHJ12_002965"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACHJ12_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4990721.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACHJ12_002966"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACHJ12_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4990722.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACHJ12_002967"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACHJ12_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4990723.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHJ12_002968"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACHJ12_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4990724.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHJ12_002969"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACHJ12_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4990725.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACHJ12_002970"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACHJ12_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4990726.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACHJ12_002971"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACHJ12_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4990727.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHJ12_002972"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACHJ12_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4990728.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHJ12_002973"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACHJ12_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4990729.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACHJ12_002974"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACHJ12_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4990730.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACHJ12_002975"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACHJ12_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4990731.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHJ12_002976"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACHJ12_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4990732.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHJ12_002977"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACHJ12_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4990733.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHJ12_002978"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACHJ12_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4990734.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHJ12_002979"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACHJ12_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4990735.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHJ12_002980"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACHJ12_002980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4990736.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHJ12_002981"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACHJ12_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4990737.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16118..16486
                     /locus_tag="ACHJ12_002982"
     CDS             16118..16486
                     /locus_tag="ACHJ12_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4990738.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGGGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16497..16922
                     /gene="zntR"
                     /locus_tag="ACHJ12_002983"
     CDS             16497..16922
                     /gene="zntR"
                     /locus_tag="ACHJ12_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4990739.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16978..17196
                     /gene="arfA"
                     /locus_tag="ACHJ12_002984"
     CDS             16978..17196
                     /gene="arfA"
                     /locus_tag="ACHJ12_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4990740.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNQGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17193..17603)
                     /gene="mscL"
                     /locus_tag="ACHJ12_002985"
     CDS             complement(17193..17603)
                     /gene="mscL"
                     /locus_tag="ACHJ12_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4990741.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17733..19109)
                     /gene="trkA"
                     /locus_tag="ACHJ12_002986"
     CDS             complement(17733..19109)
                     /gene="trkA"
                     /locus_tag="ACHJ12_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4990742.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19131..20420)
                     /gene="rsmB"
                     /locus_tag="ACHJ12_002987"
     CDS             complement(19131..20420)
                     /gene="rsmB"
                     /locus_tag="ACHJ12_002987"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4990743.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20466..21413)
                     /gene="fmt"
                     /locus_tag="ACHJ12_002988"
     CDS             complement(20466..21413)
                     /gene="fmt"
                     /locus_tag="ACHJ12_002988"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4990744.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21428..21937)
                     /gene="def"
                     /locus_tag="ACHJ12_002989"
     CDS             complement(21428..21937)
                     /gene="def"
                     /locus_tag="ACHJ12_002989"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4990745.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22067..23191
                     /gene="dprA"
                     /locus_tag="ACHJ12_002990"
     CDS             22067..23191
                     /gene="dprA"
                     /locus_tag="ACHJ12_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4990746.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23163..23636
                     /gene="smg"
                     /locus_tag="ACHJ12_002991"
     CDS             23163..23636
                     /gene="smg"
                     /locus_tag="ACHJ12_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4990747.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23665..24207
                     /locus_tag="ACHJ12_002992"
     CDS             23665..24207
                     /locus_tag="ACHJ12_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4990748.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24212..24784
                     /gene="tsaC"
                     /locus_tag="ACHJ12_002993"
     CDS             24212..24784
                     /gene="tsaC"
                     /locus_tag="ACHJ12_002993"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4990749.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24789..25607
                     /gene="aroE"
                     /locus_tag="ACHJ12_002994"
     CDS             24789..25607
                     /gene="aroE"
                     /locus_tag="ACHJ12_002994"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4990750.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25604..25861
                     /locus_tag="ACHJ12_002995"
     CDS             25604..25861
                     /locus_tag="ACHJ12_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4990751.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25837..26391)
                     /locus_tag="ACHJ12_002996"
     CDS             complement(25837..26391)
                     /locus_tag="ACHJ12_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4990752.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6856 a         6347 c         6935 g         6570 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtcca cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgcgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagacc acgcttattt tttgacatcc cgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atccgacctg cgattagaac
     1321 tggaaggcgc gaaggatctc cgtgaggcta tcgcgtatgc cgacagcgtt catgactatg
     1381 ttagccgtga tatgatgatc gagatccttg ccgatgaaga aggtcatatt gactggctcg
     1441 aaactgagct ggatctcatc ggtaaaatcg gtttacaaaa ttacctgcaa tcacaaataa
     1501 aagttaaaga ttaacggcct gccagcaaac tatcccaacc tacaacgaaa cccgcagccg
     1561 ccagaaatgg cccaaacggc agcgggtttt ttaatgactc ttttcctctc atgagcagac
     1621 caacaactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggcaagaagg
     1681 tccaggtatg ccaggcacca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgacg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaataa
     1801 tcgcccccca taacgcatcg gctaaaccgt caggattaca aacctgagag aacaaaagac
     1861 ctgaccagag taacgggcag gtaaatctgt caggtaacaa gccatgcttt gcatcccata
     1921 aaaaaagtag gacagataaa caagcgtaca aaatcagaaa gggaagggta gcgtccataa
     1981 aacatcctct ataattggag tatcaccatc gaataactcc ggatgctcag caacagagaa
     2041 atcgctgatt tgttaacctg gttccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatac agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcacaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctatcgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtact ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcctgg gtattgtaaa accatggaac tctacctggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcgtt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttataaa
     9961 ggcaagggtg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactctg caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcctgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaaatctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctgtctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccaggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 attactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtatttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cctggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtgcgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaatattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacctg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgcct ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 acgtaaaaaa acccgccccg gcgggttttt ttatacccgt agcatcccca cttatctaca
    16081 atagctgtac tctttttgtt catcccctgg agtatttatg tggttacttg accagtgggc
    16141 agagcgccat atagcagaag cgcaagcgaa aggtgagttt gataacctgg caggtggcgg
    16201 cgaaccattg atactggatg atgattctca cgtgccaccg gaattacgtg cggggtatcg
    16261 cttgctgaag aatgccggtt gcttaccgcc agaacttgag caacggagag aagcaattca
    16321 gcttctggat attctcaaag gtatccgtca cgatgatccg caatatcaag aggttagccg
    16381 tcgattgtca ttactggaat tgaagctgcg acaagctgga ttgagtaccg attttttacg
    16441 cggcgattat gctgacaagt tgttggacaa aatcaacgat aactagtgga gtatgtatgt
    16501 atcgcattgg tgagctggca aaaatggcgg aagtaacacc tgacacgatt cgttattacg
    16561 aaaaacagca gatgatggag catgaagtgc gtactgaagg cgggtttcgc ctgtataccg
    16621 aaagcgatct ccagcgattg aaatttatcc gccatgccag acaactaggt ttcagtctgg
    16681 agtcgatccg cgagttgctg tcgatccgca tcgatcctga acaccatacc tgtcaggagt
    16741 caaaaggcat tgtgcaggaa agattgcagg aagtcgaagc acggatagcc gagttgcaga
    16801 gtatgcagcg ttccttgcaa cgccttaacg atgcctgttg tgggaccgcc catagcagtg
    16861 tttattgttc gattcttgaa gctcttgaac aaggggcgag tggcgttaag agtggctgtt
    16921 gattttttgc actggcagga ttacactcgc gccgtaaaat aaccaattgg agtttttatg
    16981 agccgatatc agcatactaa agggcagata aaggataatg cgatagaagc attactacat
    17041 gatcccttat tccgacagcg cgtagagaaa aataagaagg ggaaaggcag ttacatgcga
    17101 aaaggaaaac atggcaatca ggggaactgg gaggccagtg gcaagaaagt aaatcacttt
    17161 tttaccactg gtcttctgct ttcaggcgct tgttaagagc ggttattctg ctctttcagc
    17221 aaatcacgaa tttctgccag taatacttct tctttagttg gtgcaggtgc ggcagctggt
    17281 tcttcttttt tccgattcag tttgttgatt agcttaatcg ccataaagat ggcaaaggcc
    17341 acaatcagaa aatcaaagac gttttgaatg aagacaccgt aatgcatcac aacagcaggg
    17401 atatccccct gcgcatcgcg tagcgtgaca gcaaactgtt taaagtcgat cccgccaatt
    17461 aataagccca gaggtggcat gatgatatcg gcaaccagtg aagatacaat cttcccgaat
    17521 gccgcaccga taatgacacc caccgccaaa tccaccacgt tcccgcgcat cgcaaattcg
    17581 cgaaattctt taataatgct catgttattc tccctatgaa gccgacaacc ataagtctaa
    17641 caaatgttaa gccattttcc tgccaggaat gaatttaatt aatccttgat aatcaaaggg
    17701 aaataataag gcgtcattag acgccttatt aattacaaga agaaagggct tggctggaag
    17761 agtctttcga cgtcggtaat aaacttttta tctgtgagga acataattac gtgatcgcct
    17821 tgctcaatgc gcagattgtc attggcaatc atcacgtcgt ttccacgtac caccgctcca
    17881 ataatcgttc ctggcggtag cttgatttcg tcaatgactc tgccgacaac gcgtgaggtg
    17941 ctttcatcac cgtgagcaac ggcttcaata gcttctgcta cgccgcggcg caatgaggaa
    18001 acaccaacaa tatctgcttt tcgcacatgg ctaagcaacg cagaaatagt tgcttgttgt
    18061 ggtgaaatcg caatatcgat cacgctcccc tgaaccagat ccacataagc gcgacgctgg
    18121 atcaatacca tcaccttttt cgcacccata cgtttggcaa gcatggcgga catgatattg
    18181 gcctcgtcat cgttggtgac ggcaataaac agatcaactt gatcgatatg ttcttcggcc
    18241 agcagttctt gatccgacgc atcaccaaaa aagacgatcg tattctgcaa cttttccgcc
    18301 agttcggcag cgcgctgctg attacgttcg atgagtttaa cgctgtaatc tttttccaga
    18361 cgacgcgcca gccctgcacc gatattaccg ccaccaacca gcatgatccg cttatacggt
    18421 ttttccagtc gctgtaattc actcatcacc gcgcggatat gctgtgaagc ggcaataaag
    18481 aacacttcat caccagcttc cacaatggtc gaaccttgtg gacgaatggg gcgatcgtgg
    18541 cggaaaattg ctgccacacg agtatcgata tgtggcatat gttcgcgcat ggtcgaaagt
    18601 gcgttaccaa tcagcgggcc gccataatag gctttaacca cagcgaggct gactttaccc
    18661 tcagcgaagt tcaccacctg caatgcgccg ggatactcaa tcagtcggta aatattatcg
    18721 ataaccaact gctctggtgc gatcagatga tcaatcggta cagcatctga atgaaatagc
    18781 ttatcggcat cgcgcacgta gtctggtgag cggatgcgag cgatgcgatt aggggtgttg
    18841 aaaagtgagt aggctacctg gcaggcaacc atattggttt catctgaact ggttacagca
    18901 accagcatat cggcgtcgtc ggcacctgcc tcccgcaata cgcgtggatg agagccatgc
    18961 ccctgcacga ctcgcaggtc aaatttatcc tgcaaggtcc gcagacgctc accgttggta
    19021 tcgacaacag tgatatcgtt gttctcgcca accaggtttt ccgccagtgt gccgccaacc
    19081 tggccggcac ccagaatgat aattttcatc agtcgcgacc cgttatctca tcactttttg
    19141 attagcttag cgtaaaagaa gccgtcgccc tcttcggcac caggcagatt ttgtttaccc
    19201 ggttgctctg gtgttcctgt ttcgcaaagt tcggcatcag cggtacgttg caaaaaggct
    19261 ttaatctgca ggctattctc ttccggtaac accgaacagg tggcatagac cagggttcca
    19321 ccggatttta aatgcggcca aatggcgtcg agaatttcag actgcaattg cgcgagttcc
    19381 gggatatcgc gatcgcgacg taaccattta atatccggat ggcgacgaat cacaccggtt
    19441 gctgaacaag gcgcatctaa taaaatgcga tcaaactgtt gctcgccaca ccattgggaa
    19501 gggtagcggc catcaccttg tttcacggtc gctttcatac caaggcgttt taaattgtcg
    19561 taaacgcgag agaggcgctg ttcgtcaata tcaaccgcaa caacctgcgc ttctggtgcc
    19621 acctcaagga tatgcgttgt tttaccgccg ggggcggcac aaagatccag aatgtgttca
    19681 ccgttttgtg gcgcaagcca ggtcatgcaa ccttgtgctg atgcatcctg aacggtaacc
    19741 catccgtctt caaaaccagg tagcgcatga acaggtgcag gtgtttccag ccgtacagca
    19801 tcagggtaat ccgcatgcgg gaaacctttc attcctgctt catccaacaa tgcaagccag
    19861 ctgtcgcggg aatgatgcgt acgattaacg cgcagccaca ttggcggacg ctggttattg
    19921 gcttcgacga tggattgcca ctgctctgga tacgcttttt gcagacgctt cagcaaccag
    19981 gaaggatgca gataacgtgc atcactggca ttaaactcgg ctaataactc ttcttgctga
    20041 cgctggaact ggcgtaatac accgtttatc aaccctttaa gttgcggacg cttaattgcg
    20101 atagcgcctt caaccgtttc agccagcgca gcatgaggtg gaatgcgggt ataaagcagt
    20161 tgatacaaac caaccataat caggtaatgc acggtccgct gtttgccggt catcggacgg
    20221 gccattaact tattaatcag ccagtctaac tgcgaaagcg tacgcagtac gccaaagcac
    20281 aactcttgaa gaagtgcttt gtctttatcg gaaacttttt gctggagcgg tggcagaatg
    20341 ttgcttaatg attgcccttg ctcgacgact tgttcaacgg cctgggccgc catgctacgt
    20401 aaattacgtt gttttttcat aagtataaaa atgcccggca agaccgggct tagaagagtg
    20461 gactatcaga ccagacggtt gcccggaaca aaccattccc gacgagagtt caggaggtct
    20521 tgcgcgctca tcgctttctt acccgcaggt tgtaacgaga gcaggttcag gatgccatca
    20581 ccagtcgcaa cctgaatgcc ttgtttattg gcttcaagga tcgttcctgg tgcagcgttg
    20641 gttgccgtat caatgaccga cgctttccag actttaaccg gttgtccttc aatttccagc
    20701 cagctcattg gccatgggtt gaaagcgcga atgcagcgtt caagctgcgc tgccgaaagc
    20761 gaccagtcaa tacgcgcttc ttctttactc aacttctcgg cgtaagtgac aagagtttcg
    20821 tcctgaactt ctggtttcgc cgtgccgtct gccagttgtt tcaacgtggt gataagccct
    20881 tgtgggccaa gctctgccag cttgtcgtac agcgtaccac tggtatcttc tgcagtaatc
    20941 gggcaggaga gcttatagag catgtcaccg gtgtctaaac caacatccat ttgcataatg
    21001 gtcacaccag tttctgcatc acccgcccat agtgagcgtt ggattggtgc agcaccgcgc
    21061 cagcgtggca gcagtgaacc atgaacgttg atacagccaa gacgcggcat ctccagcaca
    21121 gctttaggca gaattaaacc ataggcgacg acgaccataa catcagcctg cagatcggcg
    21181 accagttgct ggttttcttg tggacgcagg gaaacaggtt gaaaaacggg cagacctttt
    21241 tcctcagcca gaactttaat cgggctgggc atcagttttt taccgcgtcc tgccggtcgg
    21301 tctggctggg tgaacacgcc aacgacgtta tgaccagaag acaacagcgc gtcgagatga
    21361 cgcgctgcaa agtcaggtgt acccgcaaaa ataatacgta gtgattctga cacgttagtt
    21421 cttatcctta agcccgggct ttcagacgat ccagtttttc aactttctga cgaatacgtt
    21481 gttgtttcag cggtgacaga taatccataa acagtttgcc gaccaggtga tccatctcat
    21541 gctgaataca gatggctaac agaccatctg cctccagttc aaatggttta ccgtcgcggt
    21601 ccagggcgcg aattttaact ttctctgcgc gtggcactaa agcacgttgt tcagggatcg
    21661 acaggcaacc ttcttcaatg cctgtttcgc cgcttttttc taaaagctcc ggattgatta
    21721 acaccagccg ttcgtcacgg ttttccgaaa catcaataac aatgatacgt tgatggatat
    21781 caacctgggt tgccgccagg ccaatacctt cttctgcata catcgtctcg aacatatcat
    21841 cgacgatacg ctgaatttct gcattcactt cttctaccgg tttagcaact ttgcgaagcc
    21901 gctcgtccgg aatatgtaac acttgcaaaa ctgacataaa tctccagaga tgtgttcagg
    21961 agttagaaag attatttctt ctattctaga caaatccccc tctgattgac agcatcactg
    22021 accaatcgca aagattgcta aggctgctta tggcagggag ataaggatgg tcgatacaga
    22081 aatttggctg cgtttaatga gtatcagcag cttgtacggc gatgatatgg tccgtatagc
    22141 tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa ctgggcttac
    22201 attgcggcag gcacaacgct ttctttcatt tccgcgaaag agtatcgaaa gctcactttg
    22261 ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt atcctcctca
    22321 acttcaggcg acgacagatt accccggcgc actgtttgtt gaaggagaac tgcacgcgct
    22381 gcattcattt cagcttgcca tagtggggag tcgggcgcat tcatggtatg gcgagcgatg
    22441 gggacgatta ttttgcgaaa ctctggcgac gcgtggagtg acaattacaa gtggactggc
    22501 gcgtggaatc gatggtgtgg cgcataaagc ggccttacag gtaaatggcg tcagcattgc
    22561 tgtattgggg aatggactta ataccattca tccccgccgc catgcccgac tggctgccag
    22621 tttgcttgaa catggcggag ctctcgtctc ggaatttccc ctcgatgttc caccccttgc
    22681 ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg tactggtggt
    22741 ggaagcggct ttgcgcagtg gttcgctagt gacagcacgt tgtgcgcttg agcaggggcg
    22801 tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc ctcactggtt
    22861 aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa acttgcaatt
    22921 tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag atcagcaaga
    22981 cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa cacctgttga
    23041 cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac tactcgaact
    23101 ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga ggagggcatg
    23161 ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacacagaa gctgagttgc
    23221 gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggatttgat cgagaagata
    23281 tctacaatgc cctgctttgg ctggaaaagc ttgcggatta tcaggaagga ctggcagaac
    23341 cgatgcaact ggcctctgat ccactctcca tgcgtattta tacccctgag gagtgtgaaa
    23401 gactggatgc cagctgtcgt ggttttctgc ttttccttga gcagattcag gtgctcaacc
    23461 ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca gagttcgaac
    23521 tggatgatct gaaatgggta atcctgatgg tgttgttcaa tattccgggc tgcgaaaatg
    23581 cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg cattaatcat
    23641 ttctttaatt cagcataagt tgttatggcg aaatcagcac tgttcacggt gcgtaataat
    23701 gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca cggtccgttt
    23761 cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc ttcagcggat
    23821 ggacatattg tcaaagttct ggaggggcag gtttgccctg catgtggcgc aaatctggta
    23881 ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga atgcgaacat
    23941 accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg ccggacgggc
    24001 catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga tcgctacccg
    24061 gagtgtcaat ttgccattaa cttcaaaccc atagctggag aatgccctga gtgtcattat
    24121 ccactactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa
    24181 tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag gagacgctat
    24241 cgcagctgcg atagatgtcc tcaatgaaga acgtgtcatc gcctatccaa cggaagccgt
    24301 tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgcgactgt tggagttaaa
    24361 acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc agcttaaacc
    24421 ctatattgat gacaccatgc tgactgatgc gcagcgtgaa accatttttt cccgctggcc
    24481 aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg
    24541 ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt tgtgccaggc
    24601 ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac cttgtcgaac
    24661 agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg gtgaaacggg
    24721 ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg
    24781 gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc aaatcgccat
    24841 tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg cgcgtgttgg
    24901 cacccatcaa tgatttcatc aacacactga acgctttctt tcgtgctggt ggtaaaggtg
    24961 cgaatgtgac ggtgcctttt aaagaagagg cttttgccag agcggatgag cttactgaac
    25021 gggcagcgtt ggctggtgct gttaataccc tcaagcggtt agaagatgga cgcctgctgg
    25081 gtgacaatac cgatggtata ggtttgttaa gcgatctgga acgtctgtct tttatccgtc
    25141 ctggtttacg tattctgctc atcggcgctg gtggagcatc tcgcggcgta ctactgccac
    25201 ttctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc gcggaagagt
    25261 tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac gaactggaag
    25321 gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt gatattccgg
    25381 cgatcccgtc atcgctcatt catccaggca tttattgcta tgacatgttc tatcagaaag
    25441 gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt
    25501 taggaatgct ggtggcacag gcggctcatg cctttcttct ctggcacggt gttctgcctg
    25561 acgtagaacc agttataaag caattgcagg aggaattgtc cgcgtgaatc aggccatcca
    25621 gtttccggac agagaagagt gggacgagaa taaaaaatgt gtatgttttc ccgctctcgt
    25681 gaatggcatg caactgacat gtgcgatctc tggcgagagt ctggcgtatc gttttactgg
    25741 agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggatc tggaagaaga
    25801 agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggctgggtct ggttaccctg
    25861 atccagatat tcgtccttcc atttcacgta attattcgcg gaatagcgta acccagcctt
    25921 ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca gatatccgct
    25981 ctccagccgt ttattttgtg ggaccagact acccgcacca atcatcacat catcttctac
    26041 tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc caatggtgca
    26101 gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta atgggttgcc
    26161 atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct ggatattggt
    26221 gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg gccagatccc
    26281 cacatcatca gccagacgaa cgtcaccaat cacgacactg ctatcgtcga tcattacgcg
    26341 ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca tacttacctc
    26401 agcaataaat gatttactaa tgactttggg ggcattattg gccttgtgca agtcttttag
    26461 tatgcaaaaa agcaccgttt tgtgtgcgaa tgcagcaaaa agggtgaaaa aacaacaaac
    26521 agaaaaaaag atcaaaaaag tacttgtgca aaaaattggg atccctataa tgcgcctccg
    26581 ttgagacgac aacgtgaaac acttcacaag atggtcgtaa caacgaagag aaaaaatcct
    26641 gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg cgacagtgag
    26701 ctgaaagc
//
LOCUS       DAZFFI010000062        26042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFFI010000062 DAZFFI010000000
VERSION     DAZFFI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.062"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..1745)
                     /locus_tag="ACHJ12_002997"
                     /pseudo
     CDS             complement(1..1745)
                     /locus_tag="ACHJ12_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2269..4197
                     /gene="thrS"
                     /locus_tag="ACHJ12_002998"
     CDS             2269..4197
                     /gene="thrS"
                     /locus_tag="ACHJ12_002998"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4990753.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4201..4743
                     /gene="infC"
                     /locus_tag="ACHJ12_002999"
     CDS             4201..4743
                     /gene="infC"
                     /locus_tag="ACHJ12_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4990754.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4840..5037
                     /gene="rpmI"
                     /locus_tag="ACHJ12_003000"
     CDS             4840..5037
                     /gene="rpmI"
                     /locus_tag="ACHJ12_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4990755.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5090..5446
                     /gene="rplT"
                     /locus_tag="ACHJ12_003001"
     CDS             5090..5446
                     /gene="rplT"
                     /locus_tag="ACHJ12_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4990756.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5563..5686
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5569..5613
                     /gene="pheM"
                     /locus_tag="ACHJ12_003002"
     CDS             5569..5613
                     /gene="pheM"
                     /locus_tag="ACHJ12_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4990757.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5896..6879
                     /gene="pheS"
                     /locus_tag="ACHJ12_003003"
     CDS             5896..6879
                     /gene="pheS"
                     /locus_tag="ACHJ12_003003"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4990758.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6894..9281
                     /gene="pheT"
                     /locus_tag="ACHJ12_003004"
     CDS             6894..9281
                     /gene="pheT"
                     /locus_tag="ACHJ12_003004"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4990759.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9286..9585
                     /gene="ihfA"
                     /locus_tag="ACHJ12_003005"
     CDS             9286..9585
                     /gene="ihfA"
                     /locus_tag="ACHJ12_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4990760.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9686..10666
                     /gene="btuC"
                     /locus_tag="ACHJ12_003006"
     CDS             9686..10666
                     /gene="btuC"
                     /locus_tag="ACHJ12_003006"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4990761.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10729..11280
                     /gene="btuE"
                     /locus_tag="ACHJ12_003007"
     CDS             10729..11280
                     /gene="btuE"
                     /locus_tag="ACHJ12_003007"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO4990762.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11280..12029
                     /gene="btuD"
                     /locus_tag="ACHJ12_003008"
     CDS             11280..12029
                     /gene="btuD"
                     /locus_tag="ACHJ12_003008"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4990763.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYISQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12107..12571
                     /gene="nlpC"
                     /locus_tag="ACHJ12_003009"
     CDS             12107..12571
                     /gene="nlpC"
                     /locus_tag="ACHJ12_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4990764.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12818..13531
                     /gene="ydiV"
                     /locus_tag="ACHJ12_003010"
     CDS             12818..13531
                     /gene="ydiV"
                     /locus_tag="ACHJ12_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4990765.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13594..15030
                     /gene="selO"
                     /locus_tag="ACHJ12_003011"
     CDS             13594..15030
                     /gene="selO"
                     /locus_tag="ACHJ12_003011"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4990766.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLHRLARTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15034..15225)
                     /gene="hemP"
                     /locus_tag="ACHJ12_003012"
     CDS             complement(15034..15225)
                     /gene="hemP"
                     /locus_tag="ACHJ12_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4990767.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15357..16403)
                     /gene="aroH"
                     /locus_tag="ACHJ12_003013"
     CDS             complement(15357..16403)
                     /gene="aroH"
                     /locus_tag="ACHJ12_003013"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4990768.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAAHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16560..17393)
                     /gene="ppsR"
                     /locus_tag="ACHJ12_003014"
     CDS             complement(16560..17393)
                     /gene="ppsR"
                     /locus_tag="ACHJ12_003014"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4990769.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17726..20104
                     /gene="ppsA"
                     /locus_tag="ACHJ12_003015"
     CDS             17726..20104
                     /gene="ppsA"
                     /locus_tag="ACHJ12_003015"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4990770.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20161..21807)
                     /gene="fadK"
                     /locus_tag="ACHJ12_003016"
     CDS             complement(20161..21807)
                     /gene="fadK"
                     /locus_tag="ACHJ12_003016"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO4990771.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21864..22157)
                     /gene="ydiT"
                     /locus_tag="ACHJ12_003017"
     CDS             complement(21864..22157)
                     /gene="ydiT"
                     /locus_tag="ACHJ12_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4990772.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22154..23443)
                     /gene="ydiS"
                     /locus_tag="ACHJ12_003018"
     CDS             complement(22154..23443)
                     /gene="ydiS"
                     /locus_tag="ACHJ12_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4990773.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALIENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23499..24437)
                     /gene="ydiR"
                     /locus_tag="ACHJ12_003019"
     CDS             complement(23499..24437)
                     /gene="ydiR"
                     /locus_tag="ACHJ12_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4990774.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24457..25221)
                     /gene="ydiQ"
                     /locus_tag="ACHJ12_003020"
     CDS             complement(24457..25221)
                     /gene="ydiQ"
                     /locus_tag="ACHJ12_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4990775.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25537..>26042
                     /locus_tag="ACHJ12_003021"
     CDS             25537..>26042
                     /locus_tag="ACHJ12_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO4990776.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         6501 a         6203 c         6772 g         6566 t
ORIGIN      
        1 atgttttcat gatttttagc ggccaacagt gtgaataatt gacgttgact aaaagaatat
       61 ttttttgcaa aagtactcag tgttgatata taagataaca cgacgtcttt attacccttg
      121 ctcatggcga tgtataaagc aggggttcca tgtgctagct ttgctcaact tgtatttaaa
      181 ggcgatgcca ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat
      241 tgcggcgtat aatcctggct caccatcatg tattttcatg gctaaaatac tcatcgccag
      301 ttttttgttt aaaagctctt gtgctattgg agtggttaat gcattcagaa agatatcgat
      361 tatttcatcg aatccataca acatggagat ataaagtcca ggtgtttcgt tagcactttt
      421 agtttgcagt aatttaacca gattatcttc atgaatgatg tgattatcta ccagtgactg
      481 aatttcttgc ataaatattt tcacgttgtc agtatgtctg ttcatcatag ccaaaaataa
      541 agcaggtgta ccaacgctat tctgcgcagt tattatttca atgatttctt tttcagggag
      601 tatgggcaac tgtttgaata atgtaataag ctgttgagtc tggcctgtag ccattattaa
      661 caacattgcc gatggatgta taggggcaaa tggcattcgt gatgcaatac ccgcgaaaat
      721 agcccctgga actcccacag gtaatagggg cagaatagca attgcacagt tgcgaacata
      781 cctatcagcc caggtctgag catcgggacc tgaactgatg aacttatcaa gcgaaagaaa
      841 gccccgtgct gtcctgtggg tgtctctgac ggcaacgttg gtatcgttcg ggtcgtaaac
      901 ggacacaaca tagtttaatt gtccatacac tgactgcttt attcgtagcc ttactgacaa
      961 agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg ccatcattcg
     1021 acaaaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc gactccctgc
     1081 aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg actaagggga catgctgctg
     1141 tatggattca acggaggagt aatgtttaat caggttgact ttgccttttc catttctcaa
     1201 tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag aaggagattg
     1261 caaaaaacat gcactaccat tgagatttac tgttgggtgt atagtttttt ctctatggat
     1321 ttttttagac agtttcatac aatggttacg atcttcagca gttaactgtt cctgttcaat
     1381 ataatccagt atgcttcgca aatggttagc tttaataggc gtggtttcca gtaataagga
     1441 aatgaaatct ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg
     1501 gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt tcgacagttt
     1561 gcggtaaatt cctgaataac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt
     1621 agggtgatga gctggcgcgc aaatttatta ataggctctg atgaaggtgt tggtatgtcg
     1681 ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat atcgttttga
     1741 ctcataatct attcgcctta tatatatctg gaggttttga ggatgatgat taattattat
     1801 cgccagaacg aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt tatcattgtg
     1861 atatgaccac ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa
     1921 cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac
     1981 gcaccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa
     2041 ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat
     2101 cgctcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac
     2161 caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct
     2221 ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat gcctgttata
     2281 actcttcctg atggcagcca acgccattac gaccacgctg taagccccat ggatgttgcg
     2341 ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg
     2401 gttgatgctt gcgatctgat tgaaaacgac gcacaactgt ccatcattac cgccaaagac
     2461 gaagacggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca cgcgattaaa
     2521 caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg tttttattac
     2581 gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga gaagcggatg
     2641 catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg gcacgaagcg
     2701 cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga cgaaaacatc
     2761 gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat gtgccgcggt
     2821 ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac ggcaggggct
     2881 tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac ggcgtgggca
     2941 gacaaaaaag cgcttaacgc ttacctgcag cgcctggaag aagccgcgaa acgcgaccac
     3001 cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcgcc gggtatggta
     3061 ttctggcaca acgacggctg gaccatcttc cgtgaactgg aagtgtttgt tcgttctaaa
     3121 ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg
     3181 gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacgtc ttctgagaac
     3241 cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt caaccagggg
     3301 ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg ccaccgtaat
     3361 gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca ggatgacgcg
     3421 catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat ccgtttagtc
     3481 tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc cactcgtcct
     3541 gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg
     3601 ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt ctacggtccg
     3661 aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag
     3721 ctggacttct ctttgccttc tcgtctgagc gcttcttatg taggcgaaga caatgaacgt
     3781 aaagtaccgg taatgattca ccgcgcaatt ttggggtcga tggaacgttt catcggtatc
     3841 ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccagttca ggttgttatc
     3901 atgaatatta ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa actatcaaat
     3961 gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa aatccgcgag
     4021 cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt ggaatcaggc
     4081 aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg
     4141 atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga ggaataaggt
     4201 attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc
     4261 gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt gagtctgaga
     4321 gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag ccctaacgcc
     4381 gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa gagcaagtct
     4441 tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa attccgtcct
     4501 ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag
     4561 ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc
     4621 ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt ggtcgaatcc
     4681 ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa gaagaaacag
     4741 taaggccttc aagtagcact gtctgtggag ccttcgggct tcacaggctt tgttcgcctt
     4801 tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat taagaccgta
     4861 cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca caagcacgct
     4921 aacctgcgtc acattctgac caaaaaagcg accaaacgta aacgtcacct gcgtccgaaa
     4981 gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc
     5041 gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata tggctcgcgt
     5101 aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta
     5161 ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca aagctggtca
     5221 gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcgcg
     5281 tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca atggcctgaa
     5341 aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat tcgacaaagt
     5401 agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca gttgaaagag
     5461 ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt atcgccgtag
     5521 ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct
     5581 attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc gtgagaagag
     5641 aaacggaaaa cagcgcctga aagcctccca gtgaggcttt ttttgtatgc gcgttttgaa
     5701 aaataaaaag cctgtctcat caggtgtaat tgtcgcagcc agtctggaca cggccagcgc
     5761 ggagaaaccg gagcgtattt aagtacgtga gaatttcgag cactgcccgg gaccaaaatg
     5821 gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt caccactgac
     5881 acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa ggcggccatt
     5941 agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa
     6001 gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga gcgtccggca
     6061 gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa
     6121 gcggaactgg aaagcgcagc gctgaatgcg cgtctggcgg cggaaacgat tgatgtctcc
     6181 ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac catcgaccgt
     6241 atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga aatcgaagac
     6301 gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg cgctgaccac
     6361 gacactttct ggtttgacac tacccgcctg ctgcgtaccc agacctctgg cgtacagatc
     6421 cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg cgtttatcgt
     6481 aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct gattgttgat
     6541 accaacatca gctttaccaa cctgaaaggc actctgcacg atttcctgcg taacttcttt
     6601 gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgttcaccga gccttctgca
     6661 gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggttg cgggatggtg
     6721 catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg tttcgccttc
     6781 gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct gcgttcattc
     6841 ttcgaaaatg atctgcgttt cctcaaacag tttaaataag gcaggaatag attatgaaat
     6901 tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat gcgctggcga
     6961 atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc ggtagcttcc
     7021 acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct gacaaactgc
     7081 gtgtgacaaa agtgaatgtt ggcggcgatc gcctgctgga catcgtctgc ggtgcgccaa
     7141 actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg ccgggtgatt
     7201 tcaaaattaa agcggcgaaa ctgcgtggtg aaccgtctga agggatgctg tgctcctttt
     7261 ctgagctggg aatttctgac gatcatagcg gcattatcga gctgcctgcg gatgcgccga
     7321 ttggcaccga catccgtgaa tacctgaaac ttgatgacaa caccatcgaa atcagcgtga
     7381 cgccaaaccg tgccgactgt ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga
     7441 atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc gacgacacgc
     7501 tgccgattac agtcgaagcg ccggaagcct gcccgcgtta tcttggccgt gtggtaaaag
     7561 gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt cgttgcggga
     7621 tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa ctgggccagc
     7681 cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg atggcgaaag
     7741 agggcgaaac gttggtactg cttgatggca ctgaagcgaa gctgaatgct gacactctgg
     7801 tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc gaacactctg
     7861 gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc ccgctgtcta
     7921 tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt tatgagcgtg
     7981 gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca
     8041 tctgcggtgg tgaggctggt ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc
     8101 gtgcaaccat cactttacgt cgtagcaaac tggatcgcct gatcggccat catattgcgg
     8161 atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg
     8221 agtggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa gatctggtcg
     8281 aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta caggcaagcc
     8341 tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca
     8401 acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga
     8461 tgatccatcc aggcgttgaa gccttactgc tgccaagccc gatctctgtt gaaatgtcag
     8521 caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc
     8581 agcagaaccg tgtacgcatt ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac
     8641 cgttgggcat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag
     8701 agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc gatcttgaat
     8761 ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg
     8821 cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt ggttttgttg
     8881 gggttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg
     8941 aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct
     9001 tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc gcagcggata
     9061 ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg
     9121 tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc ctgatcctgc
     9181 aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc aaatgtgtag
     9241 aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc gcttacaaaa
     9301 gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga tgccaaagaa
     9361 ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga acaggtgaaa
     9421 ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg acgtaacccg
     9481 aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag acccgggcag
     9541 aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga tctaactaaa
     9601 aaggccgctc tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc
     9661 atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac agcgacaaaa
     9721 tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct taagcctttg
     9781 cgccggtgaa caatggatct cgccaggtga ctggttttct cctcgtggcg aactgttcgt
     9841 ctggcagatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg cgctggctat
     9901 atccggcgca gtaatgcagg cgttgtttga aaatcctctg gcagaacctg gactacttgg
     9961 cgtctctaac ggcgcaggcg tagggcttat cgccgcggta ttgcttgggc aagggcaact
    10021 cccaaactgg gcactagggc tgtgtgcgat tgctggcgcg cttatcatca ctttaatact
    10081 cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg gcgttgcatt
    10141 agggattatc tgtagcgcac taatgacgtg ggctatctac ttttccacct ctgttgattt
    10201 acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc ggcaaagctg
    10261 gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa
    10321 tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt ggttctggcg
    10381 caatgtgctg gtggcagcga ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc
    10441 tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt taaccgatca
    10501 tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc tggccgatgt
    10561 tgtagcgcgc ttggcattag ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt
    10621 gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc aaaaagacgg
    10681 tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat gcaagattcc
    10741 attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga gaagtacgcc
    10801 ggtaatgtgc tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag
    10861 cagttggaga atattcagaa agcctgggcc gatcgaggtt ttgtggtgct gggattcccg
    10921 tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac ttactgtacc
    10981 accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg cgaaggacgc
    11041 catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc
    11101 ggattctatg cccgtatggt cagcaaaggt cgtgcaccgc tgtaccctga tgatatttta
    11161 tggaattttg agaaattttt agttggcagg gacggattgg tcatccagcg tttttccccg
    11221 gatatgacgc cggaagaccc cattgtgatg gaaagcatta aactggcgtt ggcaaaataa
    11281 tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg ccactttctg
    11341 gcgaggttcg ggctggggag atcctgcatc tggtggggcc gaatggcgcg ggtaagagta
    11401 ccttgcttgc acgaatggcc ggaatgacca gcggtaaggg aagcattcag ttcgcggggc
    11461 aacccctgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat atttcacaac
    11521 agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat cagcacgata
    11581 aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat gacaaactcg
    11641 gacgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg
    11701 tgctgcaaat cacaccacaa gccaatcccg caggtcaatt gctgcttctt gatgagccga
    11761 tgaacagtct tgatgttgcg caacaaagtg cgttagacaa aattctgagc gctctgtgtc
    11821 agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacattg cgtcatgcgc
    11881 atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga gaagaggtgc
    11941 tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg gatatcgaag
    12001 gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat cgggtaatta
    12061 cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc gtttctgcct
    12121 tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac cgccgccaaa
    12181 tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc tacaaagctg
    12241 gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact gttcgggatt
    12301 tgtggttgta acgatgcgcg atcgtttcga tttgcagctg ccccgagaaa ccaaagaaca
    12361 agcctctatc ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc tggtcttttt
    12421 caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca acaaccaatt
    12481 tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg tctattggca
    12541 gaaaaatttc tggcaggcga gacgaattta gtatgtcgaa aaatggacgg cgatgataca
    12601 tcgccgtcca tttactcctg aatacagtag tattttgcag acaaaaattc cgtaaatgga
    12661 ttattcgttt ataatcagga taatctaaaa aaaggacccc tgaaaaagtg gttaacatgg
    12721 aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc ccttttagcc
    12781 ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt tggagaatct
    12841 ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc ttgtcggcgt
    12901 tgagttaatt acccatttct ccagtgaaga tggcacggtg cgtatcccga cgagtcgggt
    12961 tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt tggaattact
    13021 aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta atttaacgcc
    13081 acaagtggct acattattat tagagaggga taattacgca ggcgaactat taaaatatcc
    13141 gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa aagataatcg
    13201 cggtctttta tccttatcgc aagtgtatcc ccttgtgtta ggtaatttgg gagcaggcaa
    13261 tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtcatgctgg ataaaagttt
    13321 tattcagcaa caaatcacgc accgctcctt cgaaccgttt attcgtgcga tccaggcgca
    13381 aatttcccct tgttgcaatt gcatcatcgc gggaggcatt gatacggcag aaatattggc
    13441 acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg ccgttcccat
    13501 aaatcagata acgacgttgg ttcagcgata acccttctgt ttgctggtgt ttaagacgag
    13561 agtaaccgtc tacactatca aacaggagga tctatgaccc tgtcttttgt tacccgctgg
    13621 cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa taatgcccgg
    13681 ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc gctgtttaaa
    13741 aatgctgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc actggcgcag
    13801 gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt
    13861 ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct gaaaggtgct
    13921 ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc gacgatacga
    13981 gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg cgcgttaagt
    14041 atcgtcacca gcgattcgcc agtgtatcgg gaaacggtag agccaggtgc gatgctgatg
    14101 cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta tcgccgcgag
    14161 ccggaaaagg ttcgtcagtt agctgacttt gccattcgtc attactggtc atatcttgaa
    14221 gatgatgagg acaaataccg cctctggttt agcgatgttg tcgcacgtac cgcatcgtta
    14281 attgcccaat ggcagacggt cggctttgct catggggtga tgaataccga taacatgtcg
    14341 ctgctggggc tgacgcttga ttacgggccg tttggttttc tggatgatta cgaacccggt
    14401 tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca acctgctgtc
    14461 gcgttgtgga atttacatcg tctggcgcgg acattgtcac catttgttgc cgtagatgcc
    14521 ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg acaacggatg
    14581 cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact gaatgaatta
    14641 ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat gctgagtctg
    14701 accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt
    14761 gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag cgatagtgag
    14821 cgtcagcagc tgatgcaaag cgttaaccca gctctggtgt tgcgcaactg gttggcgcaa
    14881 cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct gcatgaggcg
    14941 ttgcgaaatc ctttcagcga cagagctgat gactatgtca gtcgtccacc tgactggggt
    15001 aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca gcttgccagc
    15061 ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt tgccatccgg
    15121 acctaacagt gtctggctgg aaattcgccg aataggctgc ggggaagcgg tcttgtgatt
    15181 ggcatccgtt tcaggcgtga gttttctgct atccgtataa cgcataaaaa ttaataataa
    15241 ttgatataac aatgattctt attatcaata ttatggctga ccagagcaag cagtaatgtg
    15301 ctgagaagac aacaagttgc gcagaaatgc ccatcccatc aggaatgggc acgcactcag
    15361 aagcgggtat ctaccgcaga ggcaagtttt tcgaccaggc gttcggtatc ttcccagccc
    15421 agacacgggt cggtaatgga ttgaccgtaa gtgagcggct gactgccgac gattttttgc
    15481 gttccttcgc gcaggaaact ttccgccata attccagcaa tcgccgtaga gccattgcgg
    15541 atttgctgac aaatatcctc acaaacttct aactggcgac ggtgctgctt ctggcagtta
    15601 ccgtggctga aatccaccac cagatgttca ggtaaatcaa actcgtgcag cgtatcgcag
    15661 gctgcggcga tatcatcggc atgataattc ggttttttgc cgccacgcat aataatgtgg
    15721 ccatacgggt tgccgctggt ctgatagatg gtcatctgac catttttgtc tggcgagagg
    15781 aacatatggc tggcgcgggc tgcgcggata gcatccacag caatccgcgt attgccatcg
    15841 gtaccatttt taaaacctac cggacaggag agtgccgaag ccatttcacg gtggatctga
    15901 ctttcggtag tacgtgcgcc aatcgcgccc caactgatta aatcagcaat aaactgaccg
    15961 gtcaccatat cgaggaactc agtcgcggta gggacaccca gctcatttac ctgtaaaagt
    16021 aatttgcgcg ccagctccag accgtgattt acccgatagc tgccgtttaa atctggatcg
    16081 gagattagcc ctttccagcc gacaacagtt cgtggttttt caaaataggt gcgcattacg
    16141 atttccagcc gtgactggta ctggttgcgc agcgactgca gacgggtggc gtactccatc
    16201 gcagcggtga gatcgtggat cgagcagggg ccaataatga ccaacagtcg cttatcttca
    16261 ccattcagta ttttttcaat tctgcggcgg gagtcggtga catgggcggc gacgccaggc
    16321 gttacgggat accgtagcgc gagttcggcg ggcgttacca ggctctcaat acgcgcagta
    16381 cggagttcgt cagttctgtt cattacaagt ctcagaaatt tgttgcttct acggcaggac
    16441 tgccgggaag tgatgcgcat cacgataaac caatcgctgc tgatttcaag tgcgaggtgt
    16501 gtcacacctc gccgggtggt tagcatgata acaaaaaaat aagctaatgc actagttctc
    16561 tagtacattc ggcgactaag gcccatgata tcgaggatct tggtggcaat ctcttctacc
    16621 gaataattgg tactgttaat ccacgggatc tgatttttac ggtacaacgc ttccacttcc
    16681 gcgacttcca tcctgcactg acgaagcgag gcatagcgac tgttctcccg acgttcctcg
    16741 cgaatcgccg ccagacgttc cgggtcgata gtcaggccga acaatttgtg ctgaagcggt
    16801 ttgagcgacg cgggtagcac cagattatcc atatcgtcgg caataaaggg gtagtttgcc
    16861 gcgcggatac caaattgcat tgccagatac agactggtgg gggttttacc acagcgagaa
    16921 acgccgagca ggatcacctg agcctggtcc agattgcgca acgaaatacc gtcatcgtgg
    16981 gcaagggtgt aatcaatcgc cgcaatgcgc gcatcatatt tattgagatt attagggtta
    17041 aggccatggg tacgatgagc aatcggcgtt ggatccagtt tcatctcttg ttgtagcggg
    17101 gcaaccagcg cctgaacgat atcctggcaa aagccttcac tttgcaagat gatggcgcga
    17161 atctccggca acacgatgga gtagaagacc agcgggcgca cacctgtctg gtgataaatt
    17221 gcgtcaatct gatccttcac tgcccgtgca cggctctcat tttcgacaaa cggcagcgta
    17281 atgctgctga tagtgacggg aaattgtgac attactgcgt gtcctaatac ctccgcagtt
    17341 attgccgtac catcagaaat ataaaaaacg tggcgatcaa cagcattatc cattttgttc
    17401 ttcccgtgat gcagacataa ttctatgaaa gcataaatta aaaacgcaca gaagcgtaga
    17461 acgttatgtc tggtttataa aatgaacctt caattttatt ttttatgaaa acagcatttc
    17521 atttttatgg tttcgtttat accgatggtt tatgtggaaa ttgtcgaaga gagcagattt
    17581 gcgcaacgct gggatcagtc ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg
    17641 tttttttcat ccggttaaat atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt
    17701 tcatttatca caaaaggatt gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg
    17761 gtataaccaa ctcggcatga atgatgtaga cagggttggg ggcaaaaatg cctccctggg
    17821 tgaaatgatt actaaccttt ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac
    17881 cgccgacgcg tttaaccagt ttctggacca aagcggcgta aaccagcgca tttatgaact
    17941 gctggataaa acggatattg acgatgttac ccagcttgcg aaagcgggcg cgcaaatccg
    18001 ccagtggatt atcgacactc ccttccagcc tgagctggaa aatgccatcc gcgaagctta
    18061 tgcacagctt tccgccgatg acgaaaacgc ctcttttgcg gtgcgctcct ccgctactgc
    18121 agaagatatg ccggacgctt cctttgccgg tcagcaggaa accttcctca acgttcaggg
    18181 ttttgacgcc gttctcgtgg cagtgaaaca tgtatttgct tctctgttta acgatcgcgc
    18241 catctcttat cgtgtgcacc agggttacga tcaccgtggt gtggcgctct ccgccggtgt
    18301 tcaacggatg gtgcgctctg acctcgcatc ttctggcgtg atgttctcca ttgataccga
    18361 atccggcttt gaccaggtgg tgtttatcac ttccgcatgg ggccttggtg aaatggtcgt
    18421 gcagggcgcg gttaacccgg atgagtttta cgtccataaa ccgacacttg cagcgaatcg
    18481 cccggctatc gtgcgtcgca ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac
    18541 ccaggagcac ggcaagcagg ttaaaatcga agacgtaccg caggaacagc gtgacatctt
    18601 ctcgctgacc aacgaagaag tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca
    18661 ctacggtcgc ccgatggata ttgagtgggc gaaagatggc cacaccggca aactgttcat
    18721 tgtgcaggcg cgtccggaaa ccgtgcgctc acgcggtcag gtcatggagc gttatacgct
    18781 gcattcacag ggtaagatta tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg
    18841 tccggtgaaa gtcatccatg acatcagcga aatgaaccgc atcgaacctg gcgacgtgct
    18901 ggtcactgac atgaccgacc cggactggga accgatcatg aagaaagcat ctgccatcgt
    18961 caccaaccgt ggcggtcgta cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc
    19021 ggcggtagtg ggctgtggtg atgcaacaga acggatgaaa gacggtgaga acgtcactgt
    19081 ttcttgtgcc gaaggtgata ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa
    19141 aagctccagc gtagaaacga tgccggatct gccgttgaaa gtgatgatga acgtcggtaa
    19201 cccggaccgt gctttcgact tcgcctgcct gccgaacgaa ggcgtgggcc ttgcgcgtct
    19261 ggaatttatc atcaaccgta tgattggcgt ccacccacgc gcactgcttg agtttgacga
    19321 tcaggaaccg cagttgcaaa acgaaatccg cgagatgatg aaaggttttg attctccgcg
    19381 tgaattttac gttggtcgtc tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc
    19441 gaagcgcgtc attgtccgtc tctctgattt taaatcgaac gaatatgcca acctggtcgg
    19501 tggtgagcgt tacgagccag atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg
    19561 ctatgtttcc gacagcttcc gcgactgttt cgcgctggag tgtgaagcag tgaaacgtgt
    19621 gcgcaatgac atggggctga ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga
    19681 tcaggcgaaa gcggtggttg aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg
    19741 gctgaaaatc atcatgatgt gtgaaatccc gtccaacgcc ttgctggcag agcagttcct
    19801 cgaatatttc gacggcttct caattggctc aaacgacatg acacagctgg cgctcggtct
    19861 ggaccgtgac tccggagtgg tgtctgaact gttcgatgag cgcaacgatg cggtgaaagc
    19921 actgctgtcg atggcgattc gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg
    19981 tcagggtccg tccgaccatg aagactttgc cgcatggttg atggaagagg ggatcgatag
    20041 cctgtctctg aacccggaca ccgtggtgca aacctggtta agcctggctg aactgaagaa
    20101 ataaaataaa tccccggcgg cgttcagtcg ccgggtttat gtgatccccg aagatgaaac
    20161 ttattcaatc tcttcacaga catcctgcgt taatcgccgc ataatatctt ttcttaacaa
    20221 aaacttttgt attttacctg aggcagtgcg cggtagtttt tcgattacca cgatatgttc
    20281 aggatattta tattttgcga cccgtttacg gctaaaaaaa gccaccacct cttccagcga
    20341 taatgaatga tgcggcgctt tcagcacgac ataagcgcat gatcgttcac ctaaacgttc
    20401 atcgggcatt gcaaccacac aggcatcgtg aattttagga tgctgcaata aaatatcttc
    20461 cacttcacgg ctgctaatat tttcgccgcc gcggacaata atatcttttt tgcgtccggt
    20521 aatttttata tagccagcct catccatacg gcagagatcg ccgctgtaat accagccttc
    20581 ttcatccagc gcacgggcgg ttaattcagg ttcatcaaaa tatcccataa acacattggg
    20641 gccacgcgag gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac
    20701 cactttaatc tctacacctg cggcagcgta accatcggtg tgcataaagc gcgacaaagg
    20761 atcatcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa cacttaataa
    20821 tttaatgccg cgctgctggc attcacgcgc cacttttttg gggattgtgg taccgccgca
    20881 aagaaagaag cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc
    20941 atagacaaac ggcgttgcgc cgagcataca ggtgcaacgc tgctgctcaa gcagcgcgag
    21001 acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcaccaatca aaaatggtgc
    21061 tgttacgcca tgcagaaagc ccgttgcgtg gccgagtggc gcaggcatca taaagacatc
    21121 ctgccaggtc agattcagcc gcgcgcaata agcccgctca ctggcgagaa tattgttatg
    21181 cgttagcatc acgccctttg gcagaccctc ggttccggag gtaaacagca ccgcagctaa
    21241 ttcatcgccg tgggtcgtta tcgccgtggt cagtggggta ttgtcggcga taatctgact
    21301 taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt gttgtagttg
    21361 tggaagctga ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt taaacaacgt
    21421 cggtgcaaag aacatttttg cctgacactt attgagcacc cataccagtt ctgcttcccg
    21481 ccaggaaggc aacagcggta cggaaaccgc accgattttc aggcatgcaa gatagataac
    21541 agtaaattca caccagccag gcagttgaaa tgcgatgcga tcgcctgatt caataccctt
    21601 cgctaacatc cagtttgcca gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga
    21661 tgctccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg tctgctgcca
    21721 gtaatcggcc agcgaagcat cgccccatag cccttgctga cgatacgccg cacgacgttg
    21781 ttcgttgaac gttaatgtca ctttcatgtt ggactctcga aacagaacat caggcccgag
    21841 atgcgggcct gtgggatgca tgattagccg tagcgaaaat caataccgaa ggtgcctgcg
    21901 ggatattgcc actgttcgag aatagtgtta ccgcacagca cccgacaggt gccgcactcc
    21961 agacaaccgg cagaatcaaa atgaatgttg ccggcgtcat cctgcttata aagtccggca
    22021 gggcaggctt tcattaattt acggaattca ttgatatcgg gatttgccgc cagaatgata
    22081 tgcgggtggc cctcatcaac atggaattta ttgacgccta atttgatgtc gacgttaacc
    22141 gtagcgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc aagttgatca
    22201 gcccaatttt cttcgcatgt cccatgatca ttttgcgtac cggctggttt ggtttgccgt
    22261 caatggtgaa catctcgttc atgatgtcgg cgaccattcg tgggtattgg ctaaacaggc
    22321 gcgggttttc tatcagcgcc gggatcttgc ggaaatgctg catatcgcgc atgacgcagc
    22381 tttgttccag ctcacgtttg tattgcgcca gactgctggc ggagaaatcc tcgcgttctt
    22441 tggcggcgat caccgttgtg gcggcagcct gagccgatgc aatggctaaa tccatgccac
    22501 gaactgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc atcacgccat
    22561 cgttaaccat ctgcggcacc attgccagac cgccttctgg caccatatgc gcggaatatt
    22621 caagcagttt gccgccgcta attagtgggc gaatggcggg gtgttgttta aaatcttcca
    22681 gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa accagcccca
    22741 aggatatgga atccttgttg gtatagagaa atcccccgcc catcaggccg tcagaagggg
    22801 aaccggcaaa cagccaggcg gcaccttcct cgcccgtaat attaaagcga tcgttgatct
    22861 gttctggtgt gaggccaata acctctttaa caccaacagc gtaatgatgc ggatcggaag
    22921 cgggaaccat tcccagcgag cggccaagca tcgagttaac gccatcagcc agaatcacca
    22981 cattcgcttc gagaatatca tccccagcct gcacgccagt gaccttgttt ccttcacgaa
    23041 ccagcgcatc gacgcgaacg cctgggataa actgcgcgcc cgcctgctcg gcttgttcca
    23101 tcaaccacgg gtccagacga ttacgcaata cggtataaga tgcgtgttgc ggaacatctg
    23161 gttgctcgcg gtgaaaatcg agggtaacgg cgctttcttc ggttaagaag gagattttct
    23221 cgcgtgtgac cttgcgttct accggcgctg atactgcaaa gcctggaatg attgcttcaa
    23281 gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg tcgccgcgtt
    23341 ctatcaccag gacatccagc ccggctcgcg ccatgaccag tgcggcaacg ctaccagcaa
    23401 cgcccgcacc gaccacaatg gcatcaaatt tgtcttccga catgcgttat gctcccttcc
    23461 cgctgcgttt acagcgggag atgtggttga aaggggaatt agcggctcaa ctggctaatc
    23521 agggcaggga cgactttgta gatatcgccc accagaccgt agtcggcata attgaagatt
    23581 ggcgcatttt tatctttatt gatggcgaca atcacttttg cgccgttgcc gccaaccata
    23641 tgctggatct gcccggagat ccccagcgtc aggtagagat cggatttcag caacacgccg
    23701 gagacaccga tataacgttc acgctccatc cagttctcgc cttcggcaat tggacgtgaa
    23761 cagccgactt cagcattcag caccgccgcc agttcgtgga ccattttcag gtcatcctgc
    23821 gccgccagac cacgaccgac gccaaccaca cgttttgctt tgctcaggtc cacgctgctt
    23881 gcggctttcg cacggcgttc gcgacagaga atttcatgac gcggagcaac ataaggtacc
    23941 gtttctgtcg ggcactgatg agaggtatca ctggtgcacg gttcctgaac accgggtgca
    24001 agggtaatga tcgccagcgg gctgttgatc ttttcctgag cgaacgctaa cccgccatac
    24061 atccggtgtt cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc atcattcacc
    24121 agcgccgcat tcagttgcac acttaaccgt gctgccagag ctttaccacg tttcgtcgcg
    24181 gccaacagca acatagcggg gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa
    24241 ttttcagtgc gttgcagcgc gtcgttttgc tcaagaacat aaatacattt tggaccataa
    24301 ggcataactg cctgcgcctg gtcggtattt tgtacaatgg catacacctg ttggccccat
    24361 tgctgagcgc cgccaaacag ttcagcataa cgttcaggat tatcgctaaa gacccagacg
    24421 ctgtttaatt gactcatcgt ttcttctccg taggcttcag ttcagggctt tcttcagatg
    24481 ttccgccagc tcggcaatgg cctccggcga atcgttatcg agaatgatgt gcttacgttc
    24541 tgtttgcggc ggtacgcgaa tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc
    24601 aatatcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa tggctttcat
    24661 cgaaggaatg cgtggcacgt taatatcgga ggtgacgcag agcacggctg gaacagagag
    24721 ttcaataact tcaacatcat cttcaagcgt gcgttcaatc accagtgtat tgccctgacg
    24781 ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccga ccagcaagcc
    24841 agcctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat cgaagccgat
    24901 cttttcaact gctgctgcca gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg
    24961 cgcatcctgc accaaataca ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt
    25021 cgagttctgc aacaatgagc cgccaacggt cagcgcggct atctcatcgt catctgttgc
    25081 aagctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg cgtcggcatt
    25141 gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc tgttcttcag gcaccagctt
    25201 aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc atctccgatg
    25261 gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg cggggttttc gacggagcaa
    25321 gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca aaagtgcaat
    25381 ttgcggcaat atagttattc catgaccgta caatcttgca ttcagtgtaa ataatcttgt
    25441 ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta tccggcatat
    25501 attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg ttttgataat
    25561 gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt tgcaattagc
    25621 gatgatccaa aatgggagtc tggacatcac gttcatgaca atgaaaccga gctgatttac
    25681 gtcaagaaag gggtagcaag gtttaccatc gattcatcgt tatatgtcgc acatgccgat
    25741 gatatagtgg tgatagaacg cggcaggctg catgcggtgg cctctgacgt taacgatccg
    25801 gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga ggaaaatcag
    25861 ctactgcaac cgcattcttg tccggtaatt gccgcggggc agggaaaaga agtcataaaa
    25921 accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca aacatcttcg
    25981 ttatgggatg catttgccta tacgttagcc attctttact acgaaaactt taaaaatgct
    26041 ta
//
LOCUS       DAZFFI010000063        25712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFFI010000063 DAZFFI010000000
VERSION     DAZFFI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.063"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHJ12_003022"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHJ12_003022"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4990777.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHJ12_003023"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHJ12_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4990778.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="ACHJ12_003024"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="ACHJ12_003024"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4990779.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="ACHJ12_003025"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="ACHJ12_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4990780.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHJ12_003026"
     CDS             complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHJ12_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4990781.1"
                     /translation="MTRTLKPLILNTGALALTLILVYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHJ12_003027"
     CDS             complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="ACHJ12_003027"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4990782.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHJ12_003028"
     CDS             complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="ACHJ12_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4990783.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHJ12_003029"
     CDS             complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="ACHJ12_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4990784.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHJ12_003030"
     CDS             complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="ACHJ12_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4990785.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHJ12_003031"
     CDS             13724..13954
                     /gene="yjdI"
                     /locus_tag="ACHJ12_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4990786.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNDKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHJ12_003032"
     CDS             13966..14238
                     /gene="yjdJ"
                     /locus_tag="ACHJ12_003032"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4990787.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFSKHEFDKTREYDDIRS"
     gene            14464..14760
                     /gene="ghoS"
                     /locus_tag="ACHJ12_003033"
     CDS             14464..14760
                     /gene="ghoS"
                     /locus_tag="ACHJ12_003033"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4990788.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14788..14961
                     /gene="ghoT"
                     /locus_tag="ACHJ12_003034"
     CDS             14788..14961
                     /gene="ghoT"
                     /locus_tag="ACHJ12_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4990789.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15080..16597)
                     /gene="lysS"
                     /locus_tag="ACHJ12_003035"
     CDS             complement(15080..16597)
                     /gene="lysS"
                     /locus_tag="ACHJ12_003035"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4990790.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16834..18291)
                     /gene="dtpC"
                     /locus_tag="ACHJ12_003036"
     CDS             complement(16834..18291)
                     /gene="dtpC"
                     /locus_tag="ACHJ12_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4990791.1"
                     /translation="MKTPSQPRAIYYIMAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18350..20497)
                     /gene="cadA"
                     /locus_tag="ACHJ12_003037"
     CDS             complement(18350..20497)
                     /gene="cadA"
                     /locus_tag="ACHJ12_003037"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4990792.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20577..21911)
                     /gene="cadB"
                     /locus_tag="ACHJ12_003038"
     CDS             complement(20577..21911)
                     /gene="cadB"
                     /locus_tag="ACHJ12_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4990793.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22276..23814)
                     /gene="cadC"
                     /locus_tag="ACHJ12_003039"
     CDS             complement(22276..23814)
                     /gene="cadC"
                     /locus_tag="ACHJ12_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4990794.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAIPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIVLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24074..24188)
                     /locus_tag="ACHJ12_003040"
                     /pseudo
     CDS             complement(24074..24188)
                     /locus_tag="ACHJ12_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24696..25022)
                     /locus_tag="ACHJ12_003041"
     CDS             complement(24696..25022)
                     /locus_tag="ACHJ12_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4990795.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(25019..25282)
                     /locus_tag="ACHJ12_003042"
     CDS             complement(25019..25282)
                     /locus_tag="ACHJ12_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4990796.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(25354..>25712)
                     /locus_tag="ACHJ12_003043"
     CDS             complement(25354..>25712)
                     /locus_tag="ACHJ12_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4990797.1"
                     /translation="FGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPI
                     PDNRENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIAR
                     HYPEALASTVNKSRKA"
BASE COUNT         6588 a         6047 c         6301 g         6776 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
      421 ccatcggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca
     1021 tcgcctgacg aaaatcaacc gcttcgtcaa tcacttcttg tgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccagcttt gtctttagtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatgt agactggttt cgcgcctgtc agcatcaagc
     1621 cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcgaaacaga ttttcaccat
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3241 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3661 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     3961 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaga
     4021 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg
     4381 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4441 cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4501 gccgaatatt catggtaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4561 gctgggctac ttcgtcacgg aatcgtcaga acattttgcc gaatatacgc cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccaaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5041 ggacccgcat actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct
     5101 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggaatta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat
     5821 tgtttcaacc atcatcactt tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6001 cggatttgct atctattatt tctcatatgt tattggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6601 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6721 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7081 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaaccagga
     7381 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7441 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgctagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacatag gagtccatac gacctgcggt ggttgggcca agtgaacctg atggataacc
     7921 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     7981 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8041 aatggtgccg gtgagcgaca aacgagtgga aaccggatat tgcgaaagct gggcgaggat
     8101 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     8161 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     8221 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     8281 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8341 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     8401 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     8461 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     8521 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8581 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     8641 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8701 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8761 gttttgcgag tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8821 ttcttcatca ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8881 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     8941 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9001 ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag
     9061 cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9121 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9181 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9241 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9301 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     9361 ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9421 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9481 gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc
     9541 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc
     9601 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9661 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9721 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9781 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9841 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg
     9901 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta
     9961 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10021 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10081 gtcgatggaa tggtgattgc cagcagatcg aggaactcaa gatggcgacc atcaaaggtg
    10141 acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10201 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10261 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10321 atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10381 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10441 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
    10501 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10561 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10621 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10681 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10741 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
    10801 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
    10861 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
    10921 tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa
    10981 atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa
    11041 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
    11101 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
    11161 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagcagt
    11221 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
    11281 atactggtga acaagatgtg gcagttgacc agccagatga ggtattttcg gcaggaaaca
    11341 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
    11401 tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
    11461 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
    11521 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
    11581 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
    11641 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
    11701 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
    11761 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
    11821 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
    11881 attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg
    11941 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
    12001 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
    12061 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgattccc ggtccatcat
    12121 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
    12181 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
    12241 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca
    12301 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
    12361 gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga
    12421 gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
    12481 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
    12541 cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga
    12601 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg
    12661 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
    12721 gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
    12781 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
    12841 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
    12901 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
    12961 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
    13021 gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt
    13081 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
    13141 cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
    13201 gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca
    13261 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
    13321 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
    13381 tgttggccag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca
    13441 ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca
    13501 tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
    13561 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
    13621 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
    13681 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
    13741 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
    13801 atgtcagcat tctggcaatt gcgtacgtgg caacgacaag ttatttaatc tcaaacgaaa
    13861 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
    13921 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
    13981 ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga aattgtcttt
    14041 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
    14101 gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
    14161 cgaaaaatta tcccactatg cccattttcg aaacacgaat ttgataaaac gcgggagtat
    14221 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg
    14281 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagagcg
    14341 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
    14401 atcatcaatc ggggttaata agttttgcgt cccagagcgt ttaatattga taggagtcat
    14461 attatggaag gtaaaaacaa gttcaatact tatgttgttt cttttgatta tccatcatct
    14521 tattcctcag tgttcttaag gttaagatca ttgatgtatg atatgaattt ctcctctatc
    14581 gtggctgatg aatatgggat accacgacaa ttgaatgaaa actccttcgc aataacgaca
    14641 tcgttagccg caagtgaaat cgaagattta atcaggctta aatgcttaga cttaccggat
    14701 attgattttg acctcaacat tatgacagtt gatgactatt tccgtcagtt ttacaagtag
    14761 ccaggaagga taagaagaac ataaaaaatg gcactattct ctaaaatatt aattttttat
    14821 gtgattggtg tgaacatatc ctttgtcatt atctggttta tctcacatga gaaaacacat
    14881 attcgtttac ttagtgcatt cctggtcgga ataacctggc caatgagtct gcctgtggca
    14941 ttactttttt ctctctttta ggagctgtag ttgagtggac ccgctggtta tcgtgaaacg
    15001 aagcgtctct cctcaaaact ggatttgcgg aacgacgaac gaagcagtcg cctgactgct
    15061 tcgttcatta aagtgaaatt tatttctgtg ggcgcatcgc cgggaagaga ataacgtcgc
    15121 gaatagtatg actgtttgta aacagcataa tcattcggtc gataccaata cccagaccag
    15181 cggttggcgg cagaccatat tccagcgcag tcacgtaatc ttcgtcatag aacatggctt
    15241 cgtcgtcacc tgcagcttta gcgttaacct gttcctggaa acgttcagcc tgatcttctg
    15301 cgtcgtttaa ttcgctaaaa ccattaccga tttcacgacc accgatgaag aattcaaaac
    15361 ggtcggtgat ttccgggtta acatcattac ggcgcgccag cggtgacact tctgccggat
    15421 attccgtaat aaaggttggc tgaatcagat gtgcttctgc cacttcatca aagatctccg
    15481 tgacaatacg tcccagcccc cagcttttct ctaccgtaat tccgatagat tcagctaatg
    15541 ctttagcagc atcaaaatta tccaggtcgg ccatatcggt ttctggacga tattttttga
    15601 ttgcttcgcg catggtgagt ttttcaaacg gtttgccgaa atcaaacaca tgctcgccat
    15661 aagtgacttt agtggtaccc agaacctctt gtgccagggt gcggaacagg gactctgtca
    15721 gttcaatcaa atcgtggtaa tccgcatacg ccatgtagag ttccatcatt gtgaactcag
    15781 gattatggcg aacagaaata ccttcattac ggaagttacg gttgatttcg aatacccgtt
    15841 caaaaccgcc tacaaccaga cgtttcagat acagctccgg cgcgatacgc agatacatat
    15901 ctaaatccag agcattatga tgggtaataa acgggcgagc agatgcccca cctggaatta
    15961 cctgcatcat cggggtttct acttccataa agccgcgcgc gaccatgaac tgacggatag
    16021 cggccagaat ttttgaacgg acaacgaacg tttgacggga tttatcgtta gcgatgaggt
    16081 ccagataacg ttgacgataa cggacttcct gatcctgcag accatggaat ttatctggta
    16141 aaggacgtag tgcttttgtc agcaggcgca actcagtaca gtgaatggaa agctcaccgg
    16201 tttgcgtctt aaacagcgta ccgcgggcac cgataatgtc acccagatcc cattttttaa
    16261 actgatcgtt ataaacacct tctggcaggc tatctcttgc aacgtacagt tgaatacggc
    16321 caccgacatc ctgcaacgtt acaaaggagg ctttccccat gatacgacgg gtcatcattc
    16381 ggccagcaac cgagacttca atgtttaagg attccagttc ctggttattc ttcgcatcaa
    16441 actcttcgtg caactggtca gaggtatggt cgcggcgaaa atcattggga aacgccacac
    16501 cttgctgacg tagtgctgcc agtttttcgc ggcgatttct cagttcatcg ttaaaatcaa
    16561 tagcctcatt ggctccccgt gtttcttgtt cagacatttt ggttcctcta aatccagctt
    16621 tcaatttagc gtagataaag agacagatcg gtacactgta ttatctgcct cgactattaa
    16681 taactcagtt tatcttatcg aaactaacgc ttacctcaag ttgatatcac taataaataa
    16741 ccatcaaaat ggtttaaatg acttattaat ctcgatttac tagtgtgatt aactgaaagt
    16801 taatttattc ataaccaatt gtttttacga cagttaatcg ttgctctcct gtatcatatt
    16861 cgtcggcgtg ctaaacagaa aacgggtagc aaaggccaat acgacgatga tcgcgacaca
    16921 agccaacgtc cactctccca tctgagaaaa gaatcgctgg taagctgcaa ttgccatacc
    16981 gctaatttgc gactctgtcg tctgctgtgc cacgacgcct gccagccagt tggcgaccgc
    17041 gcctgttgcc agcatataaa taccggttaa tacgccagac attttcagac gcgtgatttg
    17101 cgcaatcgcc accgggtcaa taaagagttc ggcaaagccc attagcgcca acccggatat
    17161 catcacgccc attgacgctt gaccgtcagc cgctgcatgt cgggcatcaa atgccaacaa
    17221 cataaagcca caagccatca gcagtaagcc aaaggcaaac ttcagccaga cgcgcaatgt
    17281 tgagttgccg cggctttctg gcgacgccag ccaggccagt acaaccccag cgagcatcac
    17341 cgcaattgca ttcaccgact ggaatagtgc tgtaggtact tcaatattga atgcctgacg
    17401 attcacaaag cgatcgataa acaagctgat ggtactgccg ccctgttgtg ccagtaccca
    17461 gaacaatgtc ccgacaaaca tcaacaatac aatttgccaa agagcacggc ggtgttcggg
    17521 gaatttgacc atcatgcggg caatgatttg tgcggcaatg aggcaaacga tcgccagcaa
    17581 atatccggac cagtcgttct ccagcagcag ggtaaaaaat actggggcta aacagagcat
    17641 caccactaac cagctccata ccggtaaggc aaatttgacg ctagtgagcg cttttttatc
    17701 catactacgc gtggactgga aatgacgatg cccgcttaag aaaatcaata aaccgataaa
    17761 catgccgcca cccgcaaggg caaagccaac atgccatcca taccactgag cagccaggcc
    17821 gcaggcgatt ggggcggcga tagaaccgat attgcccgca gcatacagca gcgaaaaacc
    17881 gccatcacgt cgatgatcgt tctcgtcgta aagctcgcca agcaaacagc tgatgtttga
    17941 tttgaataaa ccgtagccac aaataatgat tgccagcgcc agatacaggc taaaggttga
    18001 atttgtatca atgcccagta ccacatggcc aagggtcatt aacagcgcgc cggcaatcac
    18061 tgcagtgcgg ttgccgagca ggcggtcggc aagccagccg ccgagaatag gggtaacgta
    18121 aaccagagaa gcatatgcgc tgaacaggct gatggcatgg ttatcatcaa aaccaagctg
    18181 atgggtgaga tagagaatga gtaaggcacg catgccgtaa aaactgaagt actcccagat
    18241 ttggatcgcc atgatatagt atatcgcgcg cggctgtgag ggtgttttca tgtgttctcc
    18301 ttatgagcaa aaaagggaag tggcaagcca cttcccttgt acgagctaat tattttttgc
    18361 tttcttcttt caatacctta acggtatagc ggccatcagc ctgacggtat gcaccgtgaa
    18421 tatcggtttc aaagcccgga tagtgagcgc cgatttcaca cagcatctgc aggaactcca
    18481 gaaccggacg gctttcttcg gtgatcattt cacccggcat taccagagga actcccggcg
    18541 gatacggaag gatcatattg gcgttaatac gacctaccat ttcgtcgagg taaacttctt
    18601 cggtcatacc gtgcagctct ttctggaatg cagcatacgg agtcattacc atcgtcggca
    18661 gcacttcaaa tgcgcggtac atcagatccg gcagattgtg gtgaacaatc agtttgtgga
    18721 tattctgagc cagttcctga atacgcatgt tttcatagaa ttcaggatct tcacgataca
    18781 gagacggcag catgtttttc acacgcaggt tcaggtcgaa cgcacgtttg aagtcagtca
    18841 gagcacgcag caggctcagt gctttggtct tatcgatacc gatgctgaac aggaacagca
    18901 ggttatacgg accggttttc tcaacaacga tgccatgttc gtcgaggtat ttcgccacga
    18961 tgctggccgg aataccaaag tcgctcatgg tgccgtcttt ttccatcccc ggagtcagca
    19021 gggtgacttt gatcgggtca agatacatgt gctcgttatc gatgtttttg aagccgtgcc
    19081 aggtgctgtc agaacgcagc ggccagcatt cagtcgtatc gatatgatct ggctgccata
    19141 catcaaagaa ccagccatca gattccgttc tcagacgttt gatctcttta cggaatttga
    19201 tcgcacgttc aatggaaccg ttgatcagac gtttacctgc attgcctttc atcatcgccg
    19261 cagcggtttc agtggacgcc acgataccgt agtgaggaga agtggtggtg tgcatcatgt
    19321 aggcttcgtt aaaggtttct tcgtttacgt cacctttaac gtggatcatg gaagcctgag
    19381 agaacgccgc cagcagtttg tgagtggact gggtttcgta aatcactttc ccttctacac
    19441 ggccaccgct cataccgcat ttaccttcgt aaatcggtga gaagttggtg taaggcaccc
    19501 acgcggagtc aaagtggatg gatttcacat ccagtgtttt cttgatgaag tcggtgttgt
    19561 acagcagacc atcataggta gagttggtaa ttacagcatg taccggccag gttgcgtttg
    19621 gtgtttcttt cacgcgctta gcaatggtag cgtgctggaa ttcactctgt gggataccac
    19681 caagaatacc gtaagcgtta cgggtcgggc ggaaatagat tggcgtaaca tcgctcatca
    19741 tcatcaggtg ggtcagcgat ttgtggcagt tacggtcaat cagaatggtg ctgcctgccg
    19801 gagcagagta catgccaaca attttgttcg cagtggaagt accgttggtc accatgtagc
    19861 tgcggtctgc gttaaagacg cgagcgatat actgttctgc ttctttgtgt ggaccactgt
    19921 gatccagcag agaacccagt tcagatactg aaatggaaat atcagatttc atggtattcg
    19981 gaccaaagaa atcatagaac aggctaccta ccgggctttt ctggaatgca gtaccgccca
    20041 tgtgaccagg agtacagaaa gtatatttac cttcacgaac atatttaaac agtgctttag
    20101 tcagcggagg cagaatagtg ttgatatatt cgtcagtggt ctgcttgatc ttattagcaa
    20161 tatcttcagc agcacccagc gcatattcaa agaagctaat ctgtaaacgc aggtcattca
    20221 ggcttacatc gagagtggaa tacgtattag cgaacgcgta caacggcagg ttctcgttca
    20281 ttttgctaat ttcttcgcac agctcgagat tatatttatc ccagtcaaaa ataacgccgc
    20341 acagacgcgc attgttttcg atcagtttta ataagtcgtc acggtcgttc gggtaaacaa
    20401 tctggaagtt cagacgttca agcgcgcgat gaagttcacg gatgggttct tctttaaaat
    20461 aaacccccat gtgattcaat attgcaataa cgttcatagt catatctcca ggtaaaaaag
    20521 gcccctccca acacatggga caaaatgaaa ggaggagcct cggaaaatac ttttaattaa
    20581 tgtgcgttag acgcggtgtg gttatccatt gagtggctct ggcgctcgtg cattttgcga
    20641 gcgtagaaca tcaggataat caggctgacg atgaaggtac ctgccagctc gaaggagctt
    20701 gcgcccatca gcgcgatgaa gcagaacacg caacccagta cagagcagat caggctgaca
    20761 aagttgcgga tgttaacgcc ttcaaaacga atcaggtcaa cgcaagagta gaagtacggc
    20821 agcatagtca gcagtactgc gataccggtc agttcaccga acaggtcaga tgctttacca
    20881 ccggcagagt tcatcagagt gatgaggatc atcagggcag tcattttcac tgcagccagc
    20941 agcagacctt ttttcggaat accgttgctg tcgacttcac cataaacttt cgggaagtta
    21001 ccgtcgttag cggcacgtac acctgcctgg cctaccaaca tcatccagga gcccagagaa
    21061 gtcaggcacg caaaggcggt gaatgcagaa accagcggcg cagcccagtt accgaggata
    21121 gttgaagcac tgattgcaaa cggagcaccg gaagccgcca ttacagaaga cggatacata
    21181 ccggaaagca cctgagtcgc agcgatataa acaatacctg ctaaaccagt acccagcatg
    21241 gttgccagcg gaacggtacg tttcgggttt ttaaccatac cagtacttac tgctgcggat
    21301 tcaacaccca cgaaggccca caggcagagc agaatacttt taatgatcgc atgaccatca
    21361 gtggtatccg cagtattcca gttagctgca taagttgccg catcaaacca atgccagcca
    21421 acaatagcag tcatcaccac aggaataagg accagcacca gaccaatagt ggttaaacgg
    21481 cttacccaag taccgccgag catatttaca aaggtaaata cccagacgat agcaatacag
    21541 gcgatacccg ccggaacagg atcatttaat actgggaaga aggtggaaag ataagatacc
    21601 gcggtaatac caatcgccag gttaccaatc cagttagcat ggtaataaag aacacctgtc
    21661 tgaaaaccaa atgcagggga aatttctccg gcataagcaa ttgggccacc ttgttgcggg
    21721 ttttttgttg ccagtcgggc atatacatac gccagcgaca ttgcaccaat aatagagata
    21781 atccaacccc agatagcaat accaccgata cttgctaggt tcgcaggtaa taatgcaata
    21841 ccgctcccca tcatattacc ggcaacaaca ccggtacagg caaatagccc gatcttcttg
    21901 gcagaactca tgctcttctc ctaatttcat ttttgaattt ggagtccggg tcatgatgta
    21961 taactatttc ctgaccagac caaactggcg ctaagattac tcacgaaaaa aggattaatc
    22021 ctaaagatta ggtgaaataa tacaaaagtt tctgtaagtg agaacttgag gttttttatt
    22081 aacacatcag gatcgcaagt tgatatcatg aaaagataaa cattcaatgt ttacaatgga
    22141 ttgcatgaca ttctctggtt aaatttatgt aataaaaatt atgcggcaaa taaattgccg
    22201 caacatatta taccaacggg aacatacaaa aactcaacaa caaatattcc agagcataaa
    22261 tcaacccgga gttacttatt ctgaagcgag aaatttgtcg agataaggta caacataagg
    22321 aacagaagtc tggaatatac cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa
    22381 attaaaggcg gtgagatatg catcagctgc ttcccggttc atccccttca tttcataaac
    22441 cttgccaagc aatacataat ttagccagga catttcaaga acaatgccag tatttatcgc
    22501 ctggtaagac tcatctgttt taccttttac cagagcactg accgctttta tttgatatat
    22561 aatggacagg ttattcaatt ccggtagtgt aacaatgtta tctatttctg tgttcagtgc
    22621 tgctaattgt ttttcatcta aaggatgttg agaatggcgc acgatatcaa ctaatgcttt
    22681 ttctgctctc gcgtaggtaa attctgggga tgattgaaca atctcaccta ataattcact
    22741 ggcacggttc aatgatttat catcgccatg cagtaaataa tcatgtgcct gataaaaatt
    22801 agttaataac gcaccacgat gaggcaaaat ttgctggagc gtctcctgca ttcgttgtgg
    22861 ccacggttgg tttaacgctt ttgataaact ctccagtaaa tcattttgaa tcgccagctg
    22921 attaccgtta gtgatgacat aacgtttatc cagcatggtt gaaccatctg cattgtctac
    22981 caattttatc gacataaagc attgttgagc acggtattgg cgctgattaa caaacgcaat
    23041 agataatgtt ttaccggaac tgctcggttc atcaatgttg tagttgattt tgtcatgcac
    23101 cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta
    23161 agagagctga tacggggaac tccagctgtt acaactttta tttaccatat taatgtcaat
    23221 atcgcgtgga ttgagcaaaa tacgcgattt gctcatcgga agacgtgtat caagacttga
    23281 aaacgctact agtgctacac agatacctaa ggacaacagg aaaaaaaacc atacccaaaa
    23341 ggtagtgaat cgtttgcttt taactgggga ttgttcaggt ggcgttgcgg tgttttgaat
    23401 gttaagactg tgggagggag aatctgtggc aggaatcgcc tctggtatag ggggaggcga
    23461 agatagcatt atttcctctc cctcttcttc gctgtaccag ataaccggca ccattaattt
    23521 atagccgcgc tttggtacag tagcgatata gacaggacta tcttcatcat tatcttttaa
    23581 tgacttacgt agttctgaga tactctgcgt cacaacgtga ttggtgacaa tacttctctt
    23641 ccagacatta tcgataagtt catccctgct aagtacttcg ccactgtgtt gagcaaagaa
    23701 aaccagaaga tcgattaatc tcggctcaag ggtaagttga cgcccattgc ggctaatttg
    23761 gtttatggac ggagtaacaa gccattcgcc aacgcgaact acaggttgtt gcataataga
    23821 aactcattcg aaaagggaat gatgcaatga taattgccac aacctatttt taccatctat
    23881 agatgggttt atttacatat tattggtgaa tgcaagacgt tatttttacc agccataaac
    23941 ttcttgatta catagtatta cgaaaggatt ttactgagaa ccagaagtaa tattccttac
    24001 catcaaaatt catcatcttt gccaaagaaa aatgttcaga aaataatcca tggaaaattg
    24061 tccggagcgc ttactatttt aatggattgt tagtctttgc atgagcaagc ggactgatac
    24121 atttctcttt gttctcattc agaaaatctc atcagtcggc gttcagaccg gtgttgtgct
    24181 ttatggatgc gttacgctcc tgatgacgtc atttgacgtt caacaacatc acggggccgc
    24241 acgacatttc acgtcagtta gtgctatcgc tcaggaacaa attttcccga attgggatat
    24301 gcccgcaaat tgctggtgat gaggggagaa tctggttgag ttcgggagaa ttgatttgga
    24361 ggcagaacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
    24421 tatatgaatt tggtgccctg actcggaatg aaactggtta tataaccaat tgttttgtat
    24481 cgattgtttt gtctttgttg ttttcgaggc caccaatgag gcccccatgt gcaattcgaa
    24541 ttcttttgac gctataatcc ctcttttttg tgctttgctg tatcattttg tctggttcga
    24601 tatgctgaat tgcggaatcg aagtaacaac aaaatacaga gatttataaa atacctggct
    24661 acctgtgtta gggagcgtta acgcacagat tttgtttatg gctgccagaa atgatctgat
    24721 ggtttgtcgt ctggctctgc aagtaccctg ttgatatccc gggaaccatg aacgatacgt
    24781 tcgatacgaa cttcagtatc cagtaccctg aacagcagaa ggtaacgacc atacgaacaa
    24841 cctctgatcc cagcccctaa ttcgggtctt tctctgtaga gtactggcga ttcggctatc
    24901 aacagacatt gttggtaaag ctcttcggta aaactgattg cacggacagg attatcctga
    24961 gcgatccagt cacctatagc ttcaagatcg tgttctgcta atggtgaaac agtcagtttc
    25021 atattggctg ttcaccttct gccatctttt tgtatttgtg cgtcagtcgc ccgaatacct
    25081 cttcagcact tttactttca ccgctgttga tgcctgcggt aacggcgctc tggagtgatt
    25141 caagctttat ctgctgttcc cgttcttcca gagcacgaag tccggcacga atgacttcac
    25201 ttgtattgtt ataccgacca ctttctattt gttcgcggat aaacgtttca aagtaaggac
    25261 tgagggatac actggtaggc atggtgattt tctcgccgat tgattgctat caattaataa
    25321 tagttaatat tcgcgaattg taaaatacct cttttatgcc ttgcggcttt tgttcactgt
    25381 gcttgccagg gcttccgggt agtgtctggc aataatatca ttaagcctgt caaccagttc
    25441 tggtttcctg aacgtgatgt gtactgagcc tttcttaaag tatttaatac tgaacatctc
    25501 gtcttcatag tcctctttac cctgaacaga ctggatgtga tcatccagac gaagggtgat
    25561 gttttcccgg ttgtcgggga tcggtttgcc gctgaacagg tgtagcattc tttccagatc
    25621 ggcaagtcga tctgcctgtt gcccggtgat aaaatgaaac ccccaccggt cccacctcac
    25681 taggttgttg acgataattt tactgccaaa tt
//
LOCUS       DAZFFI010000064        25548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFFI010000064 DAZFFI010000000
VERSION     DAZFFI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.064"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..102)
                     /locus_tag="ACHJ12_003044"
     CDS             complement(<1..102)
                     /locus_tag="ACHJ12_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990798.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKEN"
     gene            complement(230..2191)
                     /gene="topB"
                     /locus_tag="ACHJ12_003045"
     CDS             complement(230..2191)
                     /gene="topB"
                     /locus_tag="ACHJ12_003045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4990799.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2196..3239)
                     /gene="selD"
                     /locus_tag="ACHJ12_003046"
     CDS             complement(2196..3239)
                     /gene="selD"
                     /locus_tag="ACHJ12_003046"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4990800.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3356..3907)
                     /gene="ydjA"
                     /locus_tag="ACHJ12_003047"
     CDS             complement(3356..3907)
                     /gene="ydjA"
                     /locus_tag="ACHJ12_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4990801.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4068..5924
                     /gene="sppA"
                     /locus_tag="ACHJ12_003048"
     CDS             4068..5924
                     /gene="sppA"
                     /locus_tag="ACHJ12_003048"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4990802.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6091..7107
                     /gene="ansA"
                     /locus_tag="ACHJ12_003049"
     CDS             6091..7107
                     /gene="ansA"
                     /locus_tag="ACHJ12_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4990803.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7118..7759
                     /gene="pncA"
                     /locus_tag="ACHJ12_003050"
     CDS             7118..7759
                     /gene="pncA"
                     /locus_tag="ACHJ12_003050"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4990804.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7852..9210)
                     /gene="ydjE"
                     /locus_tag="ACHJ12_003051"
     CDS             complement(7852..9210)
                     /gene="ydjE"
                     /locus_tag="ACHJ12_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4990805.1"
                     /translation="MEQYDQIGARLDRLLLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9327..10085)
                     /gene="ydjF"
                     /locus_tag="ACHJ12_003052"
     CDS             complement(9327..10085)
                     /gene="ydjF"
                     /locus_tag="ACHJ12_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4990806.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     IRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10222..11201)
                     /gene="ydjG"
                     /locus_tag="ACHJ12_003053"
                     /pseudo
     CDS             complement(10222..11201)
                     /gene="ydjG"
                     /locus_tag="ACHJ12_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
     gene            complement(11211..12158)
                     /gene="ydjH"
                     /locus_tag="ACHJ12_003054"
     CDS             complement(11211..12158)
                     /gene="ydjH"
                     /locus_tag="ACHJ12_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4990807.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12163..12999)
                     /gene="ydjI"
                     /locus_tag="ACHJ12_003055"
     CDS             complement(12163..12999)
                     /gene="ydjI"
                     /locus_tag="ACHJ12_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4990808.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(13020..14063)
                     /gene="ydjJ"
                     /locus_tag="ACHJ12_003056"
     CDS             complement(13020..14063)
                     /gene="ydjJ"
                     /locus_tag="ACHJ12_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4990809.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14080..15459)
                     /gene="ydjK"
                     /locus_tag="ACHJ12_003057"
     CDS             complement(14080..15459)
                     /gene="ydjK"
                     /locus_tag="ACHJ12_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4990810.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(15486..16562)
                     /gene="ydjL"
                     /locus_tag="ACHJ12_003058"
     CDS             complement(15486..16562)
                     /gene="ydjL"
                     /locus_tag="ACHJ12_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4990811.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(16932..17204)
                     /gene="yeaC"
                     /locus_tag="ACHJ12_003059"
     CDS             complement(16932..17204)
                     /gene="yeaC"
                     /locus_tag="ACHJ12_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4990812.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17246..17659)
                     /gene="msrB"
                     /locus_tag="ACHJ12_003060"
     CDS             complement(17246..17659)
                     /gene="msrB"
                     /locus_tag="ACHJ12_003060"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4990813.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18001..18996
                     /gene="gapA"
                     /locus_tag="ACHJ12_003061"
     CDS             18001..18996
                     /gene="gapA"
                     /locus_tag="ACHJ12_003061"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4990814.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19080..19964
                     /gene="yeaD"
                     /locus_tag="ACHJ12_003062"
     CDS             19080..19964
                     /gene="yeaD"
                     /locus_tag="ACHJ12_003062"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4990815.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(20015..20869)
                     /gene="yeaE"
                     /locus_tag="ACHJ12_003063"
     CDS             complement(20015..20869)
                     /gene="yeaE"
                     /locus_tag="ACHJ12_003063"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4990816.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(20959..21705)
                     /gene="mipA"
                     /locus_tag="ACHJ12_003064"
     CDS             complement(20959..21705)
                     /gene="mipA"
                     /locus_tag="ACHJ12_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4990817.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDIGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22141..24075
                     /gene="yeaG"
                     /locus_tag="ACHJ12_003065"
     CDS             22141..24075
                     /gene="yeaG"
                     /locus_tag="ACHJ12_003065"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4990818.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24188..25471
                     /gene="yeaH"
                     /locus_tag="ACHJ12_003066"
     CDS             24188..25471
                     /gene="yeaH"
                     /locus_tag="ACHJ12_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4990819.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         6567 a         6592 c         5952 g         6437 t
ORIGIN      
        1 attttcttta atcatccgtt caaaattact attaaatatt tcccagccat taaaagaata
       61 cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac
      121 atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc
      181 cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg
      241 ccccgcttcc ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag
      301 cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca
      361 ccggagtacg tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa
      421 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
      481 tcatgtccgg tcgcgtagcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg
      541 tggagtggat atagcgccct tttttggtga ggaaaccacg cttgaacaac agttcaataa
      601 tcccggcacg cgttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat
      661 ctttgtcctg cacaaagcgc gcaatcccgg tcatcgccga aagcagtgtt gcatcggtaa
      721 aatggcgcgg cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat
      781 cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
      841 ctaacagcgc gcgccaacct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt
      901 tggcaatgtc cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca
      961 gatactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
     1021 aactccgtgc ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc
     1081 ggttgcgtat atctggatct accactggct gcggcaacag atctggtgca tgaacgctga
     1141 tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg
     1201 aacgcggata agtgattaac ttgtgcgttt cgtacagctt ctggcagata tcaagcacgt
     1261 tctgcgcgct cagaccgaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg
     1321 gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac
     1381 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
     1441 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
     1501 gtgtcacgat atgcgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
     1561 catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc
     1621 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca
     1681 gccagtcggc acgcgctcgc gccagcgcag aaacgcacag cggtacaaac tcactgttgg
     1741 aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac
     1801 gctgcacctg ctggcgcttt tccggtgcca gttgtagata gtccagcact tcatccacca
     1861 gcagttgccc ttcgcgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga
     1921 accgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt
     1981 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
     2041 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
     2101 cgataaagcc atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca
     2161 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
     2221 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
     2281 gtagctttaa cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt
     2341 tgcggatcgc acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag
     2401 ctggcaaagt tacgttcggt gccgcctggc actgcgccca acttaatgta ctcttcaacc
     2461 cccggcaatt tcgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac
     2521 atttcgctca aatggcccag cagaccaaag cccgtaacgt cggtcatcgc ttttacgcct
     2581 tcgatgttgg caaaggacgc gcctgcgata ttcatccggc acatcacttc tgtcgccagt
     2641 ccctgatgtt ctggtttcag cagtgatttt ttctcagccg tggtaagaac gccgatcccc
     2701 agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc
     2761 tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag
     2821 tgaccgcccg ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact
     2881 tcgcgggcaa tttccgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata
     2941 atcggtttgc cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca
     3001 aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg
     3061 ccattgccca gatcgtacac cgccgcatcg tcgcgggtct cattacccac aagcaaattc
     3121 ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt
     3181 ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg
     3241 gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga
     3301 gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga
     3361 aataagttac aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcaactgcg
     3421 gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac
     3481 gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaat ccttgggcaa
     3541 cggctgccat ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg
     3601 ggactttatg attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg
     3661 gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt
     3721 ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt
     3781 gcatggactt atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac
     3841 ccgttggcgc gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg
     3901 catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt
     3961 ttgttacagc acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc
     4021 acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt
     4081 ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa
     4141 tggtacttaa cctgttcttt attttcctcg tactggttgg tgtggggatc tggatgcagg
     4201 tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg
     4261 gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc
     4321 ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc
     4381 gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag
     4441 gcggcgacca gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg
     4501 ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt
     4561 tcgccaataa aatttggctg tcaccgcaag gcgtggttga tctgcacggt tttgctacca
     4621 acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg
     4681 tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgatatgtca ccggcagccc
     4741 gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg
     4801 ctaaccggca gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt
     4861 taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac
     4921 tggcatcgag tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg
     4981 ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg
     5041 gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg
     5101 ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag
     5161 tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga
     5221 ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg
     5281 gcatggcggc atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc
     5341 ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc
     5401 tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggcg gatgtttcta
     5461 tcaccagggc actgccgccg gaagcgcagc agatgatgca attaagcatt gagaatggct
     5521 ataaacgctt tatcacgctg gttgctgatg cgcgtcattc gacgccggag caaattgata
     5581 aaatcgccca gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata
     5641 gtctcgggga tttcgatgat gcggttgcca aagcagcaga gctggcaaaa gtgaaacagt
     5701 ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt
     5761 ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg
     5821 cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac
     5881 aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg
     5941 ccgacagatc gtcggccaca ttatttttta cctcgacgaa tcctcttccc gctgtctcga
     6001 cccatatttc cttatccgca tagtatcagg tgcgctcctc ctgcctcacg tatatacttt
     6061 tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac
     6121 acgggcggga ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat
     6181 ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc
     6241 attcatgaat atacgccgtt gatggattct tctgatatga cgccggaaga ctggcagcat
     6301 attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat tctacacggt
     6361 accgacacga tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa
     6421 ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt
     6481 aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt aacgctcttt
     6541 ttcaataacc gattgtatcg cggcaaccgc actaccaaag cccatgccga tggttttgat
     6601 gcgtttgcct ctccaaacct tcctccgtta ctggaagctg gtatccatat tcgtcgtttg
     6661 aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat cacgccacaa
     6721 ccaattggcg tagtgacgat ttatccaggg atttctgctg acgtcgtgcg caattttctg
     6781 cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac
     6841 aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg
     6901 acacaatgta tgtccggtaa agtgaatatg ggtggttatg ccaccggtaa cgccctcgcc
     6961 catgccggcg ttattggcgg tgcagatatg actgtagaag ccacgctaac caaactgcat
     7021 tacctgctga gccaggaact ggatactgaa accattcgca aggccatgag ccaaaacctg
     7081 cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccatcgcg ccctgttact
     7141 ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag
     7201 tacggtagat gtcgctaacc gccttattga ctggtgccag tcgcgcggag aagcggttat
     7261 cgccagccag gactggcatc cggcgaatca cggcagtttt gccagtcagc acggtgtaga
     7321 gccttatacg ccaggccaac tcgacggttt gccacaaacc ttctggccag atcactgtgt
     7381 gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga tcgcagcggt
     7441 gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg
     7501 tcggcagaaa acctctctcg atgactggtt acgcgatcat gaaatcgatg aattgatcgt
     7561 tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg
     7621 ttataaggtg aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cccaggacag
     7681 tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg
     7741 ggaagagacg caggggtaat tttacgctgg cctacaattc tgtactggca ttgtaggcca
     7801 aataaaacac gtcagtggca catctggcaa ttgatgccat caacgaaaga tttaattcac
     7861 ctcagaaatc tcttccaacg acaccttccg cgtttcgatg ccaaaaatgg agagaaccag
     7921 cgcacaaagc aataacatga caccaagtac cataaacacc gtgatcgacc cataatgtgt
     7981 taataatgcc gcaacgccat agggcgtgaa aactgcgacg atccgtccga cggcattaac
     8041 gaaacccgaa ccgcgcaggc gtaaatgcgt tggccaaagc tccgggatat aaaccgccga
     8101 cgcgaagcaa acatacatgt ataaaaagaa gatcatcacc aggccataga ttaaaatcgc
     8161 ccactctgta gtctgaattg aatagatata gcctaacacg gcaataataa taagtaaggt
     8221 ggagccaaat aaccgacgag gaaaatgatc aataattaat gccgcaataa atattcctac
     8281 cggagcgcca atcataataa cagcggtcat taatattgat ttatcgacat caatgccgga
     8341 gttaacaaat atggtcggta tccatacggt gatggtataa agcgagatgt tcattgcaat
     8401 taaaacagta atcgcgacta atgtacgtcg taacatttca cctttaaaca ggagccagaa
     8461 agtacccttg attactttaa ccttgctgtt gctctgatac gaagtaagcg ggggtaaacg
     8521 aatactcttc tctctttcaa tttgctgctc tacttcacga agttggcttt ctgcaccggc
     8581 gatttgccct ttccctgcca gccatcgtgg cgactcgata aagtatttac ctgagagaaa
     8641 ccaggctaac agtatgccaa taccacccag cagaaacatt attcgccaac taaaaaaagc
     8701 gataaccacc acgcctatcg ccgcagacag catgggcgac cagttaccaa caaatgagag
     8761 ccgcgcggac cattttccac gcaccgtcgc ggggataaac tccgtaaatg aggcatagcc
     8821 aaccataatc agcgccccca ttcctgttcc catcaggaag cgaaagaaga tgagccagta
     8881 catatcaggg acaaaagcgg cccctgttgc agcaataccg acgatgagaa gatttatgcg
     8941 aaacgccctg cgccgcccaa agtagtcacc aataaaccca ccagtaagtg agccgatgaa
     9001 ataaccaaac attaatgccg aggtaaaggc ggcattgagg aaattatttg accatccatt
     9061 gcttaccagc tttgctaaga cgacattacc ggagtaactc aaaaaccctg ttaataacag
     9121 actaaagctt ataataccaa atatacgata atgaaaccgg gccaaaagca agcggtccag
     9181 tcttgcgcca atttgatcat attgttccat taggtgcctc ggatttgcta aaaaggtagc
     9241 aattgcggca aattcacttg ccgcatcttt atcatcattt tatttcaagg cgcaggaaca
     9301 aataatattg acaacgtgtg cttaaattac cacaccagct ggatattatt gtctttgcaa
     9361 aatgctatcc actctgcacc cggtgattta tcagttatta tgtaattaat atgactaaaa
     9421 tcagctaact ggacaaaagc tttgcgatca aacttagagt gatcaaccaa taacgcaact
     9481 tctgtcgcct gacggatcat tgtctttttg atttcagctt ccgcttcatt agagtccagc
     9541 gcgccgctgt taatatcaag acctttacaa ctcattacca tgatatcgac atgatagcgc
     9601 ctgatgatct ctttagtaat tcttccttgc agtgaaagtg tatttttgtt tagttcccca
     9661 cccgttgaaa cgactttaat ttctgactga gccagaacat gaatcgcttc cgccgagttg
     9721 gttagcaacg tcaggccact acggtcctgt aacaatttga gcaattccat tacggtacta
     9781 ctggaatcgg ctgccatggt ggttttattg tcgataaagg gtagtgcctt gcgtgcaata
     9841 agctgcttct cttcataaaa cgatgaagcg cgcttataaa aatggatatt ctccgtcaac
     9901 atcgctgtat ttaaaacagc accaccatag gttctggtca aaaagccttc atcttccagc
     9961 ttctcaagat cgcggcgaat ggtttcttcg gttacctgaa aaatcccact caaatttgag
    10021 actgtcacct ttttatcgtt ggcaaccatt tgcttaattg cctgaatcct gtcttttgcc
    10081 gccacgatta cacccctgta tctttttaca tcacattagc gcgattatcg cataaccgat
    10141 gtttactttc aaaataacct gtttgaatca cagattttca tcacagtttt cacagaaaca
    10201 gaggtgaatc gtgttgagta tttaacgctc cagggcctct gccatttccc tcatcaatgt
    10261 tgcgtctgca tccgataagt tgatattcag tgccgcgaca ttttcgcgta cctgttccgg
    10321 tgcagtagcc ccactaagaa tggagattaa atcactctgt tttaatatcc acgccagtgc
    10381 cagagtggga attgtgcact gataacgagc acaaagtggc tgccactgtt caagcatatc
    10441 aatcactttc agcatgtttt cacgctggaa ccagacttta tttgcccgag cgccgcccgg
    10501 aacgtaatca cgagtgatgg tgccggtcaa caatccctgc tctagcgggg aataaacctg
    10561 aaccacaatg ccattatcac gacatagtgg cagcagttcg ttttccattg cccggtcgag
    10621 gatactgtat ttcgcctgaa taatatccag ttcaccatat tgcagatact cgcggatatg
    10681 gtcagcatcg acgttagcag cgcctatagc gcgaattttc ccttcagact ttaactcatt
    10741 aagcactgcg acagtttcag cgatcggcgt aaaaaatggc ggcaccgact gccagtgcgt
    10801 catgtagata tcgatgtaat caatacccag acgttgcaag ctcgctgcta cctcttcgcg
    10861 gatagattcc gggtaaggtt tttatacaac tgccgatcgc caactttgtt gaataaactt
    10921 ccttttcgtt cccagacaat gccgcatttg gtttctacta caacctgttc acggggcagt
    10981 ttttttaacg cctgaccgac gataacttca ctattgccaa agttatatcc tggcgcagta
    11041 tcaatcagat taatgccaca acgatgggct tcaagaatcg tatcaataca tatttgccga
    11101 tcgagatcgc cattccatgc aggaccgccg ccaatggccc atgtccccaa ccccattcgc
    11161 gaaagcgtaa tatccgttgt gcctaaaggt atctttttca tttgccttca ttatccttcg
    11221 tattcttcca gcaattgttc gaccagcttt ctgtttttta cgccggtggt ggcaccgacg
    11281 cttagaaccg agatagccgc cgttgcattg gcaaagcgtg cgcattcacg cagatttttg
    11341 ccttctaaca gtgccgcaat aaaacctgaa gcaaagttat cgcccgcgcc aatggtgtcg
    11401 atggcggtta ttcctgcgac cgccggcacc ttcatcgtca tgtcaccacg cttgataaag
    11461 cagccgtctt tacccgtttt aatcaccacc gtttttacgc cgcacgcaag aaagcagtca
    11521 gcaatttcat ccagtgtctc tttcccggtg agtaattttg cctcggcaaa attaggaaac
    11581 agataatcga cataactcaa tgcttcgcaa atatcatcca gcgtttcatt caaccgcggt
    11641 ttgatcatat cggcacagat aatcatctgc cgggctttag cttgcgtaaa aatttctgtt
    11701 agcgctttac catccaatag tggactgttg aaaatactgg ccagtgataa taatttcgcc
    11761 tgagaaaacc gcgcaaaatc aacatcgtca atattcaatt tccacagact gccattacgg
    11821 tttgtgacaa acgtccgctc gccatcctcc gtcaccagtc caacgttaat agaggtatct
    11881 atgctgacgt cctgcttcag gctttgaata tcaatattct ctttgcggca atggtcgaga
    11941 ataaattgcc ctgcggcatc tttaccaata cgactcatta atgctgtgcg atggcccaga
    12001 cgagaaataa ttgttgcttc atttattgca tcacccccgg tggtcattgc gattctttca
    12061 agaggataag aatccacatc aaagatattt ttactgaccg gttgcaatgg aatatcaaca
    12121 atagcggcac ctatacaaat aacgtcgaga ttatccatat cattattccg ctttgccatc
    12181 agaaccgaac agtttaattt tttccaatgc ccgttctttt acagctttac gtacttcacg
    12241 ttctaaatgc aggaaaggct gatcctggtt ttctttcact gcaaccattg cggcctgaca
    12301 gagctccgta tgaatgttga ttttcgcgat acccaacgaa attgcagttt taatgtcggc
    12361 atcactgatc cccgatgcac cgtgcaaaac cagcggaaca gaaacggcat cgcgtacgcg
    12421 tttgacgacc tcaaagttca attgtggctc tgacgtataa accccatgct ggttgccgat
    12481 ggcgacagcc aatgaatcac agcccgtacg ttcaacaaat tcagccgcct ggtcaggatc
    12541 ggtatagtga tagcccgcca gcgcttcctc ataaacggtt tcattaccga catgccctaa
    12601 ttccgcctct accggaatac ccagcggatg gaagaaatcg acagcctctt tggttaaacg
    12661 aatattttct tcgaaatcaa acgcggaagc atcacgcatt aatgaattca taccatgagt
    12721 ccaggcgtta tgaataatct ccatactccg accatgatcc caatgagtta ttaccggcac
    12781 cgttgctttt tgtgccattg ataccatcat gtgagagaaa tcttcaaatg aggtgttacc
    12841 gacaaaacct gtaccaaaag aaataataac cggggatttc gcttcttcgg ctgcgtcgat
    12901 aacgcccatc aacatttctg cattccatac gttaaaatgg gcaattgcat aatgtttatt
    12961 tgtggcatcg ttttcccaat atctgatatc tgcgagcatg ttctgttctc ccgtattaat
    13021 taatcgctaa ttttaataac gcctttaata atgtcgcgtt tgttgttaac tgactcttca
    13081 aatgcctgtt gtacatcccg ataatcgtaa atatgcgtca ccatcgattt cacatcgaat
    13141 cgccctgaag aaatagcttc aatcgtgacc ggataacgat tggcatagcg gaataccgtc
    13201 tggatagtga cttcgcgatt gattttgagg aaattgattg ccgaatcgcc gggtacagta
    13261 ccaacaatca taattttacc gccgcgcatt accagataag gtgcctgttt aacggtgacc
    13321 gcagaacccg ctgtttcgaa aacaatatct gcgcccatgt cttcggtaaa ttgctgacag
    13381 cgtgcaatag tgtcttcttt tgtgccgtta ataaccactg tcgcaccaag ttgttccgcc
    13441 attatcagac gtttttccag cacatcaacg acggcaattt ccgttgcgcc caggcatttg
    13501 cacgcttgca acgtcatcaa accaatacaa cctgctccca gaataattat cttcttaccc
    13561 ggtttaacat ctgccagcat cgcggcatgc atcccgactg cggcaggctc caccagcgcc
    13621 ccttccatcg tgtccatatt gtctggcagt ttgtaagtaa agctctccgg atgacacaga
    13681 tagtgcgtta atgcgccgcg gtagttgggt tgtgtcgcca taaaatcaac gtccgggcag
    13741 atgttatatt tgccttccag acagtaacga cagtgaccgc aaggaacgcc aggttcgata
    13801 tttacccgat cccccggttt aaatttgcgc acgcggcttc ccacagccac aaccgtcccg
    13861 gcgcattcat gacccaggcc aatttcttga tttgggtctt taggcggaat aaacgggcct
    13921 gattcaaaac catgtacatc tgaaccacaa ataccgacat attctacttt aatcaaaact
    13981 tcatcttctt taggcactgg tatttctgct gaaataattt tcattgtgcc cggcacctgc
    14041 aatattgctt ttgaattttt cattttgaat ccacccgtat tatttattgg ctactgcatc
    14101 aatacttaaa ctttcaacgg agacaccttt ggtttcaatt ccaatggtgg cgatagcaat
    14161 tgcgacaaca attgaaacag cccccagaag aataaagact cccgttacgc cataactact
    14221 gagcagcact gcaacggcat aaggtgcggc aataccactg attcgcccta ccgcattcgc
    14281 cagaccggag ccacggagtt tggcctctgt cggccagatt tcagggacat acactgccga
    14341 ggcatagcaa acgtacatat agacgaaagt aatcaggaag aaaccaatca acgttatgag
    14401 caacatactg gtttgcagtg aatagatata tccgagcacc gcaatcagga ttaatagccc
    14461 cacacccatt gttttacgcg gaattttatc catcaccagc atggcaataa aaataccaaa
    14521 tggcgcgcca aacatactca tggtatttaa aacaatcgag tcttttaaat taatcccctg
    14581 ggtcatgaat attgttggca accagttaat tagtgtgtac tgcacaacgt tcatggcaat
    14641 cagcacacaa gagcctaata tcacgcgttt cagtaatact cctgtcagta aggctgaata
    14701 cggcaccgct tgtggcgctt taccgtcatc agcaataacc acaggcggca aaggtttacc
    14761 cgtctggcgt atgacgcctt cttctatact ccgcatgact ttctcggctt cttgataccg
    14821 tccgcgcgat tccagccagc gcggggattc aggaaagtag cgccaggcca gcgccgtagc
    14881 gataagcgac aatattgcag ggataagcag ttgtacccgc cagttccact ctgcactaat
    14941 cagcggcgtg agtcccatcg caatcaatga acagagcgga tatgaccagt tgccaataaa
    15001 ggaaacccga ctggaccacg ttccgcgatt tctaccgggc atatattcgg tgaaaccagc
    15061 aaacagtgta accagtaaag ctcccagccc aacgcccatc acaaaacggc aggcaataag
    15121 aaaatccata ttcggtgaga aagcaccgac aaccatcgag gcaatatgaa tggcctcata
    15181 gaggatgaac gcatttctgc gaccggtttt gtcgccaatg atgccgccca ccagcgcacc
    15241 gagaaacatt ccggcggtcg tgattgctga gaatgtggct gtggtggaat tatctgtcca
    15301 gcccaacgct ttcagctgcg cgaggatcaa gccaccaacg gcattactcc agcagacaag
    15361 caagccaaac gcgacgatgg caaacattga cgaatgccag cggcaatccg gtaagcgatc
    15421 cagccgtgca ccacaatgcg gttttgttat ctgttccatg cgataacgtt ccttcaggag
    15481 agtagttatt catcaaagtc gtaagtcatg atcactttga ttgcggtttt atcgaccatc
    15541 gcatcaaacc cttcgcgcca ttgcgacagg ccgatacgat gcgtgatcat cggtttgact
    15601 ttgatagcgc cgctggctaa tagccggata gcgttacgcc atgaggtgga gtcataggcc
    15661 atatgcccaa tgatgctttt attccaggcg gtaatgtcat taatcgagaa atcaagaggt
    15721 ttgaagccca ttccaacgcg taccacttcc ccattcgggc ggagcatttc gatggcttgt
    15781 ttcagtgcga tattggcacc ggagcattca atcaccagtc ccagattatc tttgccacaa
    15841 atttgctggc agcgcgccac cacatcttcg gtagaaccat ttactactgc cgtcgccccc
    15901 agttcttttg caaccgggaa gcggaccgcc acatcttctt gcagaccaac gacgacgata
    15961 tttaccgccc ccataatccg cgccatttgt acggaaaaca gcccgagtgg gccagtgccg
    16021 atgacgacaa catcctgacc aggaaggaat ttcgattgct gcgcgatgga tttataggca
    16081 ttacagatag ggtcaagtac ggctgcgtcc tcataatcaa caccatcagg gatttcccac
    16141 aacgcatgac gatgaatttt gagaatttca ccaggaacca gacaatattt ggaaaaacca
    16201 ccgccccagg tattgttatc cagaccaagg tttacttttt ctgtacaaca cagaaaatca
    16261 ccctgttcac aggccggaca aacgccgcaa acgtgaccgc tgttatccga cacgacgcgt
    16321 tgccccactt tccagtcttt gactttttca ccaacctgcg caatacaacc tgcgaactca
    16381 tggccgcgga tagagttaaa ctcatcagaa ccgctatcga cattgtagtg cttcatgtct
    16441 gcgccgcaga ttgccgcggc tttaatttca atcactacgt cttccgggcc acaaatgggt
    16501 tgtgggacat caatcatctt gtagccgcca aaggccttgc caaaccgagc cagtgctttc
    16561 attgcgtcat cctctgtgtt ttgttttgct tagatggcca tcatacttac ccacatgatt
    16621 tttgaggtca acattaaatc tgtaaaacac agataatgat ctgcgtttta caacttagat
    16681 cacaattacg cagaaaatgg ctgggtctgt tacaggttgg tggaaggcgg gacgcaaaaa
    16741 gagcaaattc gagagggatt accggccaaa taggtctgag acttaagttc aggagaagtc
    16801 attcaggcgt gaacgcgact gcaagagatc ggactgggac ttagccaggt cgggtcgaga
    16861 taattgagac gagtcgtggg gtcattgttg gctatcccct tcatagccgc ggcaaaaagg
    16921 ggataaacca cttacttaaa catagcaata ggctttgctg agataccaaa atcttctttt
    16981 aactgctgtt tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct
    17041 tcggtattat ggcgggcttg ccacagcatc accagttgca ggcaattctc tttttgttcc
    17101 tcggtcaacg caacgccatc aggccatttc cccagttcaa cggcggtcga caaacgctgg
    17161 tatacctcag gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg
    17221 tggaataagt tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcgg
    17281 taaagcgtaa agaggcagag ttaacacaat aacgttcgcc tgttggttgc ggcccgtcgg
    17341 gaaagacatg ccccagatgg gcatcacagt taccgcaacg aatttctatg cgctgcattc
    17401 catgtgacaa gtctttgata taacgaatgg attcttcact caccggttcg tagaaactgg
    17461 gccagccaca gccggaatca tacttggttt cggaatgaaa cagcggggca tcgcagatca
    17521 aacagtgata tacgccgtca cgcttgttat gcagtaaacg acccgtaaat ggcggctctg
    17581 tcccatgatt ctgcgttacg taaaactgca tctcggacaa attttttttc agttcttctg
    17641 ccgaaggttt attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact
    17701 gattctaaca aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga
    17761 aatggctgca cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt
    17821 cggggcgccg accccatgtg gtctcaagcc caaaggaaga gtgaggcgag tcagtcgcgt
    17881 aatgcttagg cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg
    17941 taaggttttt gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat
    18001 atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct
    18061 gctcagaaac gttctgacat cgagatcgtt gcaatcaatg acctgttaga cgctgattac
    18121 atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg
    18181 aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg
    18241 gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc
    18301 ctgactgacg aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact
    18361 ggtccgtcta aagataacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct
    18421 ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa
    18481 gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc
    18541 gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct
    18601 tcccagaaca tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca
    18661 gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta
    18721 gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt
    18781 aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta
    18841 tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct
    18901 ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac
    18961 aaagttctgg acctgatcgc tcacatctcc aaataagttg agatgacact gtgatctaaa
    19021 aagagcgact tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca
    19081 tgattaagaa aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc
    19141 gtaaactgga tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg
    19201 cattacaggg cgcacacctt ctctcgtgga aacctgcggg cgaagaagaa gttctgtggt
    19261 tgagcaacaa cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct
    19321 ggccgtggtt tggtccggcg gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc
    19381 tgccgtggac gctgaaatca catcatgaag atgctgatgg cgtagcgctg acttttgaat
    19441 tgacacaaag cgaagagacg aaaaaattct ggccgcacga ctttacgctg ttagcgcatt
    19501 tccgcgtggg taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct
    19561 ctgccctgca tacctacttt aacgtgggtg acatcgctaa ggtaagcgtc agtgggctgg
    19621 gcgatcgctt cattgataaa gtaaatgacg cgaaagaaga tgtactgacc gacggtattc
    19681 agaccttccc tgaccgtacc gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta
    19741 atgatgaagc gctgaatcgt attatcgccg taggccacca gcatcatctg aacgttgtcg
    19801 gctggaaccc gggaccggcg ctttcagtta gcatgggcga catgccggat gatggctaca
    19861 aaacatttgt ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtg accaaagaga
    19921 aacctgcaca tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt
    19981 gtgccgggtg cgatgcatcc ggcacgtatc aacatcacac catatccagc gcagtttttc
    20041 cttttggtgc cggatacgct ttatccagca tggttaattc cgctgacgaa agttcgacct
    20101 caagcacagc cgcattctgt tggacatggg caatcgtggc cgcttttgga atcgccatca
    20161 caccctgatg gctgattacc cacgccaaca atacttgtgc cgcgctgata ttgtgagcat
    20221 gtgcaatttc gttgactacc gcgtttttta acagtccatt gcgcaaccgc ccggcctggg
    20281 ctaatggact gtaagccatc accggcatct gctgttgctg gcaccagggg agtagatcgt
    20341 actcaatccc tcgtgaaccg agatggtaaa gcacctgatt agtggcacac tgatttcccc
    20401 ccggcagctg ccagagttcc tgcatatcag catgatcaag gttagaaacg ccccagcggc
    20461 ggatttttcc ctgggcgatc aatttttcca tcgctgccac agtctcttca aaagcgaaac
    20521 tgccagacca gtgtaataag tagagatcga gataatcagt attgagacgg cgtaaactgg
    20581 cttcgcatgc atttatcgct ttttgcccgc cagcattcca cggatagact ttagagacga
    20641 gaaagacgtt atcgcgcaga ccggttaatg cttccccaac caccttttcg gcaccgccat
    20701 cggcatacat ttcggcggta tcaatgaggg ttaaaccgag ctcaatgccc gcgcgtagtg
    20761 cagcaacttc tgttttgcgc tgactggcat cttcgcccat ataccatgtt ccctgcccta
    20821 tggctggcag tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc
    20881 aggtattgca ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg
    20941 ccctggaaag atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca
    21001 ggatttatcc accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcggt
    21061 accgtaaaca ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct
    21121 ccagctgtcg ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac
    21181 gccataatag tattcgttct ggttttcgct gttccactgc acaccaatac ccggagtcac
    21241 ggtcaggcca ccgttggtgt aacgatacaa ccaggccata tcccagacaa tgccgttgct
    21301 gttatccagg gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc
    21361 ataagacaga ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatc
    21421 gccaatgtct ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc
    21481 cgttgcgtca ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc
    21541 ttcatagttg attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc
    21601 aacgacacct acgcctgcgc ccagggaaaa tttaccttca gcgtgcgcta cgcctgcaga
    21661 cgttgcgata agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt
    21721 aaacaaatgt ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt
    21781 acgttttcat tttttaaagt aactgtttaa ttttcctgac gcggatgaca ccgcgcttac
    21841 agccaaatga atttagcgtt actggctacc ctggtcttta cattaattat gcaaaattta
    21901 tggatgagtt gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt
    21961 gccatctacg cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa
    22021 atggcatgag agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag
    22081 cctctactat tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc
    22141 atgaatatat tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca
    22201 ctgcaggagt ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag
    22261 cggctattga tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt
    22321 tctcgactct tttctaaccg ggtcattgca cgttatccgg catttgaaga gttttacggc
    22381 atggaagacg cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa
    22441 gagaagaaac aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct
    22501 gagcgactga aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag
    22561 cgtagcccgg tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgctcagatt
    22621 ctggaaaaag agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccatgggcg
    22681 gcaaaacgcc tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg
    22741 ccgtcaattc tgcaacaaat tgctatcgcc aaaacggaac ccggtgatga gaacaaccag
    22801 gacatctccg cgctggttgg gaaagtcgat attcgtaaac tcgaacacta cgcgcagaat
    22861 gacccggacg cctacggcta ttccggtgcg ctgtgccgcg ccaaccaagg gatcatggaa
    22921 tttgtcgaga tgtttaaagc accgataaaa gtgctgcatc cgctgttaac cgccactcag
    22981 gaaggtaact acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc
    23041 gcccactcga acgaatccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc
    23101 ctcgaccgtg tttacatcgt gaaggtgccg tattgcttac gcatttccga agagatcaaa
    23161 atctacgaga aattgcttaa tcacagtgaa ttgactcacg ccccctgcgc ccctggcacg
    23221 ctggaaacgc tgtcacgttt ttccattctt tcgcgcctta aagagccaga aaactccagc
    23281 atttattcaa agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc
    23341 aagtcgtatc aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg
    23401 acgcgttttg cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg
    23461 gcaaacccgg tccatctgtt ctacgtactg gaacagcaga tcgagcgcga gcagttccca
    23521 caagagcagg cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc
    23581 gagtttatcg gcaaagagat ccagacggcc taccttgaat cctattccga atatgggcaa
    23641 aacattttcg accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc
    23701 gatccggata ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc
    23761 gagaaaccgg cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta
    23821 ctgcgcgcca gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg
    23881 cgcacggtta tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg
    23941 tttaacgcca aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg
    24001 atggaaaaag gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta
    24061 cgtaaatcgt cttaacaacc ctggcccggt acacatgtta ccgggcctac aacgacagcg
    24121 aaccgtgggc ctgagaagcg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg
    24181 ggggcatatg acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa
    24241 tcgccagcgt tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat
    24301 taataagcgt tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga
    24361 tattagcgaa ccgatgtttc atcaggggcg tggcggtctg cgccaccgcg tgcatccggg
    24421 caatgaccat ttcgtccaga acgaccgaat tgaacgtcct cagggtggcg gcggaggttc
    24481 cggcagtggt cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca
    24541 gatttcgaaa gatgagtatc ttgatttgct ctttgaagat ttggccttac cgaatctgaa
    24601 acaaaaccaa caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa
    24661 cggcgttccg gcaaatatca gcgttgtgcg ctccttgcag aactcactgg cgcgacgcac
    24721 agccatgacg gcaggcaagc ggcgggaact tcatgcactg gaagagaatt tggccatcat
    24781 cagcaacagt gaacctgcgc agctgctgga agaggaacgt ctgcgcaaag aaattgcaga
    24841 attacgtgcc aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa
    24901 ctacgagaag cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc
    24961 cggttcaatg gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct
    25021 gttcctcagc agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaggc
    25081 gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccatcgtttc
    25141 cagcgccctg aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa
    25201 tatttacgct gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca
    25261 tgaaatcctg gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac
    25321 ccgtcgtgca catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa
    25381 ctttgcgatg cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt
    25441 tcataaacaa aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc
    25501 tgatttttag cttactgtaa attatctcat ttattacata cattagct
//
LOCUS       DAZFFI010000065        25401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFFI010000065 DAZFFI010000000
VERSION     DAZFFI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.065"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(142..225)
                     /gene="yohP"
                     /locus_tag="ACHJ12_003067"
     CDS             complement(142..225)
                     /gene="yohP"
                     /locus_tag="ACHJ12_003067"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4990820.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            449..1885
                     /gene="mdtQ"
                     /locus_tag="ACHJ12_003068"
     CDS             449..1885
                     /gene="mdtQ"
                     /locus_tag="ACHJ12_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4990821.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1938..2699
                     /gene="yohF"
                     /locus_tag="ACHJ12_003069"
     CDS             1938..2699
                     /gene="yohF"
                     /locus_tag="ACHJ12_003069"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4990822.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2829..3407)
                     /gene="yohD"
                     /locus_tag="ACHJ12_003070"
     CDS             complement(2829..3407)
                     /gene="yohD"
                     /locus_tag="ACHJ12_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4990823.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3577..4164
                     /gene="yohC"
                     /locus_tag="ACHJ12_003071"
     CDS             3577..4164
                     /gene="yohC"
                     /locus_tag="ACHJ12_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4990824.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4338..5270
                     /gene="pbpG"
                     /locus_tag="ACHJ12_003072"
     CDS             4338..5270
                     /gene="pbpG"
                     /locus_tag="ACHJ12_003072"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4990825.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5308..6750)
                     /gene="dld"
                     /locus_tag="ACHJ12_003073"
     CDS             complement(5308..6750)
                     /gene="dld"
                     /locus_tag="ACHJ12_003073"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4990826.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     REPHSVIGSSCIGASVIGGICNNSGGSLVQRGPAYTEMSLFARINEDGKLTLVNHLGI
                     DLGETPEQILSKLDDDRIKDDDVRHDGRHAHDYDYVHRVRDIEADTPARYNADPDRLF
                     ESSGCAGKLAVFAVRLDTFEAEKNQQVFYIGTNQPEVLTEIRRHILANFENLPVAGEY
                     MHRDIYDIAEKYGKDTFLMIDKLGTDKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQ
                     KFGHLFPSHLPPRMKNWRDKYEHHLLLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPE
                     EGSKAFLHRFAAAGAAIRYQAVHSDEVEDILALDIALRRNDTEWYEHLPPEIDSQLVH
                     KLYYGHFMCYVFHQDYIVKKGVDVHALKEQMLELLQQRGAQYPAEHNVGHLYKAPETL
                     QKFYRENDPTNSMNPGIGKTSKRKNWQEVE"
     gene            6946..9243
                     /gene="bglX"
                     /locus_tag="ACHJ12_003074"
     CDS             6946..9243
                     /gene="bglX"
                     /locus_tag="ACHJ12_003074"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4990827.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9454..10371
                     /gene="osmF"
                     /locus_tag="ACHJ12_003075"
     CDS             9454..10371
                     /gene="osmF"
                     /locus_tag="ACHJ12_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4990828.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10378..11535
                     /gene="yehY"
                     /locus_tag="ACHJ12_003076"
     CDS             10378..11535
                     /gene="yehY"
                     /locus_tag="ACHJ12_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4990829.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGFGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11528..12454
                     /gene="yehX"
                     /locus_tag="ACHJ12_003077"
     CDS             11528..12454
                     /gene="yehX"
                     /locus_tag="ACHJ12_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4990830.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            12459..13190
                     /gene="yehW"
                     /locus_tag="ACHJ12_003078"
     CDS             12459..13190
                     /gene="yehW"
                     /locus_tag="ACHJ12_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4990831.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13171..13278)
                     /gene="yohO"
                     /locus_tag="ACHJ12_003079"
     CDS             complement(13171..13278)
                     /gene="yohO"
                     /locus_tag="ACHJ12_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4990832.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13338..14069)
                     /gene="mlrA"
                     /locus_tag="ACHJ12_003080"
     CDS             complement(13338..14069)
                     /gene="mlrA"
                     /locus_tag="ACHJ12_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4990833.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14291..15976
                     /gene="btsS"
                     /locus_tag="ACHJ12_003081"
     CDS             14291..15976
                     /gene="btsS"
                     /locus_tag="ACHJ12_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4990834.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            15973..16692
                     /gene="btsR"
                     /locus_tag="ACHJ12_003082"
                     /gene_synonym="yehT"
     CDS             15973..16692
                     /gene="btsR"
                     /locus_tag="ACHJ12_003082"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4990835.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16739..17209
                     /gene="yehS"
                     /locus_tag="ACHJ12_003083"
     CDS             16739..17209
                     /gene="yehS"
                     /locus_tag="ACHJ12_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4990836.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17250..17711)
                     /gene="yehR"
                     /locus_tag="ACHJ12_003084"
     CDS             complement(17250..17711)
                     /gene="yehR"
                     /locus_tag="ACHJ12_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4990837.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(17836..19836)
                     /gene="yehQ"
                     /locus_tag="ACHJ12_003085"
     CDS             complement(17836..19836)
                     /gene="yehQ"
                     /locus_tag="ACHJ12_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4990838.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAVECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(19833..20969)
                     /gene="yehP"
                     /locus_tag="ACHJ12_003086"
     CDS             complement(19833..20969)
                     /gene="yehP"
                     /locus_tag="ACHJ12_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4990839.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(20962..23241)
                     /gene="yehM"
                     /locus_tag="ACHJ12_003087"
     CDS             complement(20962..23241)
                     /gene="yehM"
                     /locus_tag="ACHJ12_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO4990840.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23252..24340)
                     /gene="yehL"
                     /locus_tag="ACHJ12_003088"
     CDS             complement(23252..24340)
                     /gene="yehL"
                     /locus_tag="ACHJ12_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4990841.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5983 a         6449 c         6901 g         6068 t
ORIGIN      
        1 gcagggggcg gcggcgattg gccgccccct gcgcgctcct tgcgccagtg attatatgaa
       61 gcaatgcaca tgacaggagc gcaacaggat gagagactga aatattctcg ttattttcgg
      121 acctgatgac attaattaat tttaaaatat catcttaaat acgccagtca ccaccagtag
      181 cccaatcagg aaaataatca atacagccca gagtataatt ttcattggcg ttcctttatt
      241 tgtgagtgct gaataagtat aggaaaaaac gaagcccttt gccgttatcc ataaacgatt
      301 aagataaatc ggaagatatc attcacttag tgtatatttt tctggatatg tcgttttatt
      361 gctactgatg tattgccacg cgaaactaaa aaatatatcg caacacacgg taatctccgc
      421 gcaccttttc ttacctgatt attcccccat gaatcgtgat tccttttatc ccgccatcgc
      481 ctgttttccg ctgttactga tgctggccgg gtgtgcacct atgcatgaaa cccgccaggc
      541 gttaagccag caaacgcctg ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa
      601 tggttggcca gacagccaat ggtggctgga gtatcacgat aatcaactca cttccttaat
      661 taacaatgca ctacagaacg caccggatat gcaggtggct gaacagcgca ttcagctagc
      721 tgaagcccag gcgaaggcaa ttgctactca ggatggtccg cagatcgact tttctgcgga
      781 tatggagcgg caaaaaatgt cggcagaagg cttaatgggg ccgtttgctc tgaacgatcc
      841 ggccgcaggt acgaccggtc cgtggtacac caacggaact tttggcttaa cggcgggctg
      901 gcatctcgat atctggggaa agaatcgggc ggaggttact gcccgcctgg gtacggttaa
      961 agcacgggcg gcggaacgcg agcaaacccg ccaattgctg gctggcagcg tagcccgcct
     1021 gtactgggag tggcaaaccc aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga
     1081 gcagaacacc attatcgcga ccgatcgcca gctatatcag aacgggatta cttcttcagt
     1141 tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg cagcagctca acgatgtcgc
     1201 ggggaaaatg aaaattattg aggcacggtt aagcgcactt acaaataacc agacaaagtc
     1261 attgaagctt aaaccggtcg cgttgccgaa agtggcaagc cagcttcctg atgaactggg
     1321 gtactcctta ctggcccggc gggcagattt gcaggcggcg cactggtacg ttgagtcatc
     1381 gctaagcacc attgatgcgg caaaagcggc attttatcct gacatcaacc tgatggcctt
     1441 cctgcaacag gatgcgttgc acttaagcga tctgttccgt cattccgcgc agcaaatggg
     1501 cgttacggca ggcctgacgc tacccatttt cgatagtggt cgtcttaacg ccaatctcga
     1561 tatcgcaaaa gccgaaagca acttgtctat cgccagctac aacaaagcgg tggttgaagc
     1621 ggtgaatgac gtggcgcggg cagccagtca ggttcagaca cttgcggaga aaaaccagca
     1681 tcaggcgcaa attgagcgcg atgccttgcg tgtggtaggt cttgcgcagg cgcgctttaa
     1741 cgcgggcatc attgctggtt cccgcgtcag cgaagccaga atccccgcgc tgcgtgagcg
     1801 ggccaatggc ctgttattgc aagggcagtg gctggatgct tccattcaac tcactggtgc
     1861 gttgggcggg ggttacaaac gctgatgata tgcgccttct atacttaacg tttattcagc
     1921 gttaagtgga gaactcgatg gcacaggttg cgattattac cgcctccgat tcggggatcg
     1981 gcaaagagtg cgcgttatta ctggcgcagc aggggtttga tattggcatt acctggcact
     2041 cagatgaaga aggggcaaaa gataccgcgc gtgaggtagt tagccacggc gtacgtgcgg
     2101 agatcgtgca gctggatctc ggtaaactgc cagaaggtgc gcaggcgctg gagaaactca
     2161 ttcaacggct ggggcgtatt gatgtgctgg tgaataatgc gggcgcaatg accaaagcgc
     2221 cgtttcttga tatggctttc gatgagtggc gcaagatttt taccgttgat gtcgatggtg
     2281 catttttatg ctcacaaatt gcggcccgcc agatggtgaa acaagggcag ggcggtcgca
     2341 tcatcaacat tacgtcggta catgaacata cgccgctgcc ggatgccagc gcctacacag
     2401 ccgctaaaca tgcgctcggc gggttaacca aagcaatggc gctggagttg gtcaggcata
     2461 agattttggt gaacgcagtc gcacctgggg cgatcgctac gccaatgaac ggcatggatg
     2521 atagctacgt gaagcccgac gcggagcctt cgattcctct gcggcgtttt ggcgcaacgc
     2581 atgagattgc cagcctggta gcgtggctgt gctcagaggg cgcaaattac accaccgggc
     2641 agtcgttgat agtggatggc ggctttatgt tggcgaatcc gcagttcaac ccagaatagc
     2701 gcagtgaggt gcgtggttat ttcaatgggt aaaattttgg taggcatgat aagacgcaca
     2761 agcgtcgcat caggcatggt gcaccggatg tcggatgcgg cgcgaacgcc ttacccaggg
     2821 caatggtttt acgcctgatg atccggcttt ttcttcccgc gccgtttcag ccaccagcgc
     2881 acgcccacca ccagaaccac gaccagaatc aaccagaccc agtgcttcaa atgctggtcg
     2941 agattgtgca accacggcgc aatcacctga ccaccagcgt aaccaatagt ggtaaaaatc
     3001 aacgcccagg caaatgcgcc gagaatattc agcggcagaa agattttcgg cggcagctgg
     3061 ctggcaccaa tcagcgtcgg gccaatcacc cgaaagccat acataaagcg cgtaccaatg
     3121 acaaacagat acggatggcg ttggataagt ttctgcgccc gctcaatttt atcctgatgt
     3181 ttcgagaaac ggcgtaacag cttgccgcca aaccgccgcc cgcacagata gagcacctgg
     3241 tcgccaatca tgccgccaag cgccacagaa agtaccacca gcgggaactt taatagcccc
     3301 tgatgcgccg caacgcctcc cagcaaagtc acggtttcac cttccgccag gctaccgatc
     3361 accagcgcgg cataaccata ttgtgagata agtgtattga gatccatagg ccagtctgtc
     3421 tccctttggt tgccagtaag catacaccct gtgaatcatt cacgctgaaa aaggacgttc
     3481 atcagacagt gaaaattaca tgggcgaaca aaaaataatc acaagctgac ttatacttga
     3541 actgtcaggt tcatgaactc acaacaaggg ggcggtatga gccatgtctg gggactgttt
     3601 tcccatcccg atcgtgaaat gcaggtgatt aatcgcgaaa acgaaacgat ttctcaccat
     3661 tacacccacc acgttttgct gatggcggcg attccggtga tttgcgcctt cattggcact
     3721 acacagattg gctggaattt tggcgatggc actatcctga agttatcctg gtttaccgga
     3781 ctggcgctgg ctgtcctgtt ttatggcgtt atgctggctg gggttgcggt aatggggcgg
     3841 gtcatctggt ggatggcgcg taattatccg cagcgtccgt cacttgcgca ctgcatggtc
     3901 ttcgcgggct acgttgcaac tccgctgttt ttaagcggtc tggtggcgct ttatccgctg
     3961 gtctggttgt gtgcgctggt cggcacggtg gcgctgtttt acaccggcta cctgctttac
     4021 cttggtattc cgagcttcct gaatatcaac aaagaggaag gccttagctt ctctagttcg
     4081 acgctggcga ttggcgtgct ggtactggaa gtgctgctcg ccctaaccgt tatactatgg
     4141 ggctacgggt atcgcctgtt ctagaaagtt ctttgacgtt gcattgctgg cgtaaatgtc
     4201 agcaacgcag catttcctca cgattctcct ttgacgatcg cactttttgc tcgttatgat
     4261 ggcgtcgcta gcctcagtaa atccttactc cggcggtgcg caacccgtgc gcgtgaacca
     4321 ctatctgaat gctcatcatg ccgaaatttc gagtttcttt atttagcctg gccctgatgc
     4381 tggctgtgcc tttggcaccg caggccgttg cgaaaacggc agccgctacc accgcttcac
     4441 aaccggaaat tgcctccggt agcgcgatga ttgttgatct gaataccaac aaagtgatct
     4501 attcgaacca cccggatctg gtgcgtccga ttgcgtctat cagcaaatta atgaccgcga
     4561 tggttgtgct ggatgcacga ctgccgctgg atgaaaaact aaaagtggat atcagccaga
     4621 cgccggagat gaaaggggtc tattcgcgcg tacgactgaa tagcgaaatc agccgtaaag
     4681 atatgctgtt gctggcgctg atgtcttcag aaaaccgcgc ggcggcaagc cttgcgcacc
     4741 attatcccgg tggttacaaa gcctttatta aggcaatgaa tgcgaaagcg aaatcgctcg
     4801 gaatgaacaa cacgcgcttt gttgaaccta ccggattgtc ggtgcataac gtttcaactg
     4861 cccgtgactt aaccaaactg ctcattgcca gcaaacaata tccgttgatc gggcagttaa
     4921 gtactacccg ggaagatatg gcaaccttct ctaacccgac gtatacgctg ccgttccgca
     4981 atactaatca tctggtgtat cgcgataact ggaatattca gttaaccaaa accggcttta
     5041 ccaatgcggc gggccattgt ctggtgatgc gtacggttat caataataaa ccggtggcgc
     5101 tggtagtgat ggacgcgttt ggcaaatata cccattttgc cgatgccagc cgcctgcgta
     5161 cctggattga aaccggtaaa gtgatgcctg tgccggcagc agcgttgagc tataaaaaac
     5221 aaaaagccgc ccaaatggcg gcggcggggc agacggcaca gaacgattaa gtgaattcgg
     5281 atggcgatac tctgccatcc gtaattttta ctccacttcc tgccagtttt tccgtttact
     5341 ggttttaccg atccccggat tcatgctgtt ggtcggatcg ttctcgcgat agaacttctg
     5401 caacgtctcc ggtgctttat acaaatgacc gacgttatgc tcggcagggt actgcgcgcc
     5461 gcgctgctgt agcagttcca gcatctgttc ttttaacgca tgcacatcca cgcctttttt
     5521 cactatgtaa tcctgatgga agacatagca cataaaatgg ccgtaataga gcttgtgcac
     5581 cagctggctg tcgatctccg gcggtaaatg ctcataccac tcggtgtcgt tacgccgcag
     5641 agcgatatcc aacgccagaa tgtcttcgac ttcatcggaa tgcaccgcct gataacgaat
     5701 tgctgcgccc gcagcggcga aacggtgtaa aaacgctttg ctgccttcct ccggcgtaca
     5761 gacaaagaaa tcgccttcgg cctgtttgaa ataatccacc aaccacgatt tggcttcgcc
     5821 cacgccatcg cccgccattt ttaacagcag atgatgctcg tatttatcgc gccagttttt
     5881 catgcgcggc ggtaaatggc tggggaacag gtgaccgaat ttttgcatcg cacggtcagt
     5941 aaaatgcgga cggaagaatt tcactttctc cagcatcgca tcggtgcgtc ccttgagatt
     6001 aaagaagaac ggcatcttgt cggtgcctaa cttatcaatc atcaggaagg tgtctttgcc
     6061 gtatttttcc gcaatatcgt agatatcccg gtgcatatat tccccggcaa ccggcagatt
     6121 ttcgaagtta gccagaatat gacggcggat ttcggtcagc acttccggct ggttggtgcc
     6181 gatataaaac acctgctgat ttttttccgc ttcgaaggta tctagacgta ccgcaaagac
     6241 ggccagcttc ccggcgcaac cagaagattc aaataaccga tccggatcgg cgttataacg
     6301 tgcgggcgtg tcggcttcaa tatcacgaac gcggtggaca tagtcataat cgtgggcgtg
     6361 acgaccatcg tgacgcacat cgtcatcttt gatgcgatca tcatccagct tgctaaggat
     6421 ctgctccggc gtttcgccca gatcaatccc cagatggttc accagcgtca gtttgccgtc
     6481 ttcatttata cgcgcgaata acgacatttc ggtatacgcc gggccacgtt gcaccagcga
     6541 gccgccggag ttgttacaaa taccgccgat aaccgatgcg cctatacacg acgatccaat
     6601 caccgagtgc ggttcgcgtc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
     6661 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
     6721 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
     6781 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
     6841 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
     6901 accattttcg ctgcctcgtc gggcaacaga ggaagaaaaa tccatatgaa atggctatgt
     6961 tcagtaggaa tcgcggtgag tctggccctg cagccagcac tggcggatga tttattcggc
     7021 aaccatccat taacgcccga agcgcgggat gcgttcgtca ccgaactgct taagaaaatg
     7081 acagttgatg agaaaattgg tcagctgcgc ttaatcagcg tcggcccgga taacccgaaa
     7141 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
     7201 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
     7261 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
     7321 ctggcctcgt cttttaacct cgatgcagtg aaaacggtcg gacgtgtctc tgcttatgaa
     7381 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
     7441 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aacaatgggt
     7501 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgctactc ggtgatgacc
     7561 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
     7621 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
     7681 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
     7741 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
     7801 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
     7861 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtattactcg
     7921 aagtatctgc ctgggttgat caaatccggc aaagtgacga tggaagagct ggacgatgct
     7981 gcccgccatg tactgaacgt taaatatgat atgggattgt ttaacgaccc atacagccat
     8041 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
     8101 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
     8161 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
     8221 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
     8281 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
     8341 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
     8401 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
     8461 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
     8521 actattccgc aaagccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
     8581 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
     8641 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
     8701 tttggcgatt acaacccgtc cggcaagctg ccaatgtcct tcccgcgttc tgtcgggcag
     8761 atcccggtgt actacagcca tctgaatacc ggtcgcccgt ataatgccga caagccgaac
     8821 aaatacactt cgcgttattt tgatgaagct aacggggcgt tgtatccgtt cggctatggg
     8881 ctgagctaca ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
     8941 gacggcaaag tgactgccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
     9001 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
     9061 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
     9121 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
     9181 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
     9241 taatgcttta gtaagggcgc gacgtttgcg ccctttgtag gccggataag gcgctcgcgc
     9301 cgcatccggc aggaaacgcc ggaccgaata tgcctcttaa gtatttatct gattctgcaa
     9361 ctgaatcctt ccgctcaagc taaccccgcc attatcaact atgcttttct cttaattcgc
     9421 tgaaaacaac agtaaaagaa tgaggaaagc atcatgccac tcttaaagct ctgggcaggt
     9481 tcactggtta tgttggcagc cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc
     9541 ggttcaaaaa tcgataccga aggtgcgcta ctcggcaata tcattttgca ggtgctggaa
     9601 agccacggcg taccgacggt gaataaagtg caactcggaa cgactcctgt ggtgcgcggg
     9661 gcgattactt ccggtgaact ggatatctat ccggaatata ccggcaatgg cgctttcttc
     9721 tttaaagatg aaaacgatgc agcgtggaaa aacgcgcagc aaggttacga gaaagtcaaa
     9781 aagctcgatt cggagcacaa caagttaatc tggctgacgc ccgcgcctgc aaataacacc
     9841 tggactatcg ccgtgcgtca ggatgtggca gagaaaaata aactcacttc gcttgccgac
     9901 ctgagtcgtt atctgcaaga gggcggcacc ttcaaactgg cagcctcggc agagtttatc
     9961 gaacgcgccg atgcgttacc cgcgtttgaa aaagcctatg gctttaagct cggtcaggat
    10021 cagttgctgt cactggctgg tggcgacacg gcggtgacga tcaaagccgc tgcccagcaa
    10081 acctctggcg ttaatgctgc aatggcttac ggcactgacg gcccggtagc ggcgctgggg
    10141 ctgcaaacct taagcgatcc gcaaggcgtg caacctatct acgcgcctgc accagtggtg
    10201 cgtgagtcgg tgctgaaaga gtatccgcaa atggcacagt ggctacagcc agtcttcgcc
    10261 agcctcgatg caaaaacatt gcagcaactg aatgccagca ttgcagtgga aggactggat
    10321 gccaaaaaag tggctgccga ctacctgaaa caaaaagggt ggacgaagta atttcccgtg
    10381 acttatctac gtattaatcc tgttctggcg ctgctgctgt tgctgacggc aatcgcagca
    10441 gcgctgccgt ttatcagcta cgcgcctaat cgtttagtct cgggtgaagg gcgacatctc
    10501 tggcagctgt ggccgcaaac gatctggatg ctggtgggct ttggttgcgc ctggctgaca
    10561 gcctgtttta ttcccgctaa aaaaggcagc atttttgcac tcattctggc gcaattcgtc
    10621 ttcgtattgc tggtgtgggg agctggaaaa gcggcgaccc aactggcgca aaatggcagt
    10681 gcgctggcgc gtaccagcct cggcagtggt ttctggctgg ctgcggcgct ggcattgctg
    10741 gcctgtagcg atgccatccg ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat
    10801 atgcagattg ccattattcc gctgtggttg ctgtactccg gcacgcttaa cgatctctcg
    10861 ctaatgaaag aatacgccaa ccgtcaggat gtgtttgacg acgcgctggc acaacatctg
    10921 acgttgctgt ttggtgcggt gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg
    10981 tgctactttt ccactgcgcg gcagggggca attttttctc tgctcaatgt cattcagacc
    11041 gtgccttcgg tggcgctctt tggcctgttg attgcgccgc ttgccgcgct ggtgacggcc
    11101 tttccgtggc tggggaagct cggcatagca ggaaccggaa tgacacccgc actgattgcg
    11161 ctggtgctct atgccttgct gccgctggtg cgcggcgtgg tagtaggctt gaaccagatc
    11221 ccgcgcgatg tgctggagag cgccagagcg atggggatga gcggggcgca gcgattcctg
    11281 catgttcagt taccgctggc gttaccggta tttctgcgca gcctgcgggt ggtgatggtg
    11341 caaactgtag gtatggcggt gattgcggcg ttaatcggcg caggcggttt tggtgcgctg
    11401 gttttccagg ggctgctaag cagcgccatt gatttagtgt tgctgggggt gatcccggta
    11461 attgttctgg cggtgctgat cgatgccctg ttcgatttgc ttatcgcact gctgaaggtg
    11521 aaacgtaatg attgaattta gccatgtcag caaactgttt ggcgcacaaa aagccgttaa
    11581 cgatcttaat ctcaattttc aggaagggag tttttcggtg ctgattggca catctggctc
    11641 cggcaaatcc accaccttga aaatgattaa ccgcctggtg gaacatgaca gcggagagat
    11701 ccgctttgcc ggagaagaaa ttcgctcgct gccagtactg gagttgcgcc gccggatggg
    11761 ctatgccatt caatctattg gcctgttccc ccactggagc gtggcgcaaa acatcgctac
    11821 cgtgccgcaa ttacaaaaat ggtcgcgggc acggattgac gatcgtatcg acgaattaat
    11881 gacgctactg gggctggagt caaatttgcg tgagcgttat ccgcatcagc tttccggtgg
    11941 tcagcagcaa cgtgtgggag tggcgcgcgc actggctgcc gatccgcaag tcttactgat
    12001 ggatgaacct tttggcgcac tggacccggt aacgcgcggc gcgttgcaac aagagatgac
    12061 gcgcattcac cgtttgctgg ggcgcactat tgtgctggtc actcatgata ttgatgaggc
    12121 gctaaggctg gcagaacatc tggtattgat ggatcacggt gaagtggtgc agcaggggaa
    12181 tccgctgacg atgctgactc gtccggcgaa tgattttgtc cgccagtttt ttggacgtag
    12241 tgaactgggg gtgcgcctgc tttcgttacg tagtgtggcg gattacgtgc gtcgcgaaga
    12301 acgggcagaa ggtgaggcac tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt
    12361 tgtcgcgcgg ggatgcgagg tgctgccggt gatgaacacg cagggccagc cttgcggcac
    12421 gctgcatttt caggatctgc tggaggaggc gtaagcgtat gaaaatgttg cgcgatccgc
    12481 tgttctggct cattgctttg tttgtggcac tgattttctg gctgccttac agccagccgc
    12541 tgtttgctgc cttgttccca caactgccac gacccgttta tcagcaagaa agttttgcag
    12601 ctctggcact ggctcatttc tggctggtgg gaatttcgag tttgtttgcg gtgatcattg
    12661 gcactggtgc cggaattgct gtcactcgcc cgtggggcgc ggaatttcgc ccactggtgg
    12721 aaactattgc cgccgttgga cagacttttc cgcctgtcgc agtgctggcg attgccgttc
    12781 cggtgatcgg ctttggtctg caaccagcga ttatcgcctt gatcctttac ggtgtgctgc
    12841 ccgtcctgca ggcgacactt gccgggctgg gagcgatcga tgccagcgtg acagaagttg
    12901 cgaaaggtat gggaatgagt cgtggtcagc gactgcgtaa ggtcgagcta ccgctggcgg
    12961 ctccggtgat tctggcgggc gtgcgaactt cggtgattat caacattggt acggcgacga
    13021 tcgcctcaac ggtaggggcc agcacgctgg gtacgcccat catcatcggg cttagcggat
    13081 ttaataccgc gtatgtgatc cagggggcgt tactggtggc actggcggcg atcatcgcag
    13141 accgcctgtt tgaaaggctg gtgcaggcgc ttagccagca cgcaaaataa aggtataacc
    13201 tgcgagcatg acgccaccaa ttccgccgaa cgccataaac aggaacaggg cgatgacccc
    13261 aattttagct atgcgcataa tgcactcctt atgttaacga aaggattgta cagtaaagcg
    13321 catttgttaa cgaatcatta aatgccgagt gggaaaatat catggccttg ttcttgccaa
    13381 ctggtgagtt gctgctgttg ggcggaggtt cgattttcac cgcaccacac cagcaatgta
    13441 cgaccttcga atagttcagg gcgtagttga ttgagcgagt gggcgaggac atcaatgcgc
    13501 catccttgtt gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc
    13561 cagccaacca ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg
    13621 atgtagttga tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg
    13681 cactgaagcc gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa
    13741 tcctgaccgc gctctttgat ccacgttcgc aggctatgca gattgccgct ttgcaggtag
    13801 gtcagtaatg tttcttgctg atcgcgccag ccgttctgca catcaacatt ttcattactg
    13861 agcagcattt taactttgct gacctgcacg ccgttgtcga tccagcgttt gatctcgcgg
    13921 atccggtcaa tatcggcatc gttgaacagt cgatgaccgc cgtctgtccg ttgcggtttc
    13981 agcaacccgt aacgcctctg ccacgcgcgt aacgtgacag ggttaatatc acaaagcaac
    14041 gccacttcac caattgtgta aagcgccatc gtctcaccct tgctcgcgag gtcccggttt
    14101 aactttagac gcagttttgc gaaccaggta gttttgcccg ttttttgtgc atctataggg
    14161 tgattttatt tttgccaggc gattttgagt gatcgtactc acgaattctc atttttctgc
    14221 aagagttcaa agaaagttaa acgcaggcaa tgtatgttac gcgttttaaa gggaagtgtg
    14281 gtttgcgggt atgtacgatt ttaatctggt gttgctgctg cttcagcaga tgtgcgtttt
    14341 tttagtcatt gcgtggttaa tgagtaaaac gccattattc ataccgttaa tgcaggtcac
    14401 ggttcgtctg ccgcataaat ttctctgcta catcgtcttt tccatcttct gcatcatggg
    14461 cacctggttt gggttgcaca ttgacgattc tattgccaat acccgtgcga taggcgcggt
    14521 aatgggcggc ttactcggcg gtccggtcgt cggtgggctg gttggtctga ccggcggctt
    14581 acatcgatat tcgatggggg gcatgaccgc gctaagttgc atgatctcaa ccatcgttga
    14641 aggattgctc ggcggcctgg tacacagcat cctgatccgt cgcgggcgca ctgataaagt
    14701 ctttaacccc attaccgccg gtgccgtcac gttcgtcgct gaaatggtgc aaatgctgat
    14761 catcctggcg atcgcccgac cttatgaaga tgcggtgcgt ctggtgagta atattgctgc
    14821 gccaatgatg gtcaccaata ccgtcggcgc ggcgctgttt atgcgtatat tgctcgataa
    14881 acgcgcgatg tttgaaaaat acacttcggc tttttctgcc actgcgctga aagtggcagc
    14941 ctcgacggaa ggcattttgc gacaggggtt taacgaagtg aacagcatga aagtggctca
    15001 ggtgctgtat caggagctgg atattggtgc agtcgcgatt accgatcgag agaaattgct
    15061 ggcctttacc ggaattggtg acgaccacca tttacccggc aaaccgattt cttcgactta
    15121 caccttaaaa gcgattgaaa ccggtgaagt ggtctacgct gatggcaacg aagtacctta
    15181 tcgttgctct ttgcatccgc aatgcaaact ggggtcgacg ctggtaattc cgttgcgtgg
    15241 cgaaaatcag cgggtgatgg gcaccatcaa attgtatgaa gccaaaaacc gtttattcag
    15301 ttcaatcaac cgcacgctgg gcgaggggat tgcgcaactg ctttcggcgc agatcctcgc
    15361 cgggcaatat gagcggcaaa aagcgatgct cacccagtca gagatcaaac tgcttcacgc
    15421 ccaggtgaac ccccattttt tgtttaatgc gcttaacacc attaaagcgg tgatccgtcg
    15481 cgacagcgaa caggccagcc agctggtgca gtatctttcc acttttttcc gcaaaaactt
    15541 aaagcggcct tcggagtttg ttactctcgc cgacgaaatt gaacatgtga acgcttatct
    15601 gcaaattgaa aaggcgcgct tccagtcgcg gttgcaggtc aacattgcta ttccgcaaga
    15661 attatcccag cagcaattgc ccgcgtttac cctgcaaccg atagtggaaa acgccattaa
    15721 acatgggaca tcacaactgc tggatacagg gcgagtggca atcagcgccc gacgtgaggg
    15781 gcaacatttg atgctggaga tcgaagacaa tgccggtttg tatcaaccgg taaccaatgc
    15841 cagtgggctg gggatgaatc tggtggataa gcgtttacgt gaacggtttg gcgatgacta
    15901 tgggataagc gtcgcctgtg agcctgatag ttacacccga ataacgttac gactaccatg
    15961 gagggacgag gcatgattaa agtcttaatt gtcgatgatg aaccgttagc acgggagaac
    16021 ctgcgtgtat ttttgcagga gcagagcgat attgaaatcg ttggagagtg ttcaaacgcc
    16081 gtggaaggga tcggcgcggt gcataaactg cgcccggatg tgctgtttct cgatatccag
    16141 atgccgcgca tcagtggtct ggaaatggtg gggatgctcg acccggaaca tcgtccgtat
    16201 attgtttttc tcaccgcgtt tgacgaatac gctattaaag cctttgaaga acatgccttt
    16261 gattatctgc tgaagccaat tgatgaagcg cgactggaga aaacgctggc gcgtttgcgt
    16321 caggaacgca gcaagcagga tgtttcgctg ttaccggaaa atcaacaggc gctgaaattt
    16381 atcccttgta cggggcatag tcggatttat ttgctgcaaa tgaaagatgt ggcatttgtc
    16441 agcagtcgga tgagcggtgt ctacgttacc agccacgaag ggaaagaggg ctttaccgaa
    16501 ttgacattac gtaccctgga aagtcgtaca ccactactgc gctgccatcg tcagtatctg
    16561 gttaacctcg cgcatttaca ggagattcgt ctggaagata acggccaggc cgagttgatt
    16621 ttgcgtaatg gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt aaaagaggcg
    16681 attggcctgt aaaagactgc taaaatggct ttttgcctca tcaacacctg aaggcctcat
    16741 gctaagtaac gatattctgc gcagcgtgcg ctacattttg aaagccaata ataatgacct
    16801 ggtgcgtatt ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg ccgtctggct
    16861 acgtaaagaa gacgaagagg gttttcagcg ttgtccggac attgttttgt cgtcattcct
    16921 caatggcctg atttatgaaa aacgcggcaa ggatgagtct gctccggcac tggagccgga
    16981 acgtcgcatt aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt cgctgaaaac
    17041 cgatgacatt ctggctatcc tcaccgaaca gcagttccgc gtttcgatgc cggaaattac
    17101 agcgatgatg cgcgcaccgg atcataaaaa cttccgcgaa tgcggcgatc aatttttacg
    17161 ttattttctg cgtggactgg cagcgcgcca gcatgtgaag aaaagctaag acgggtatgg
    17221 cggccatgcg aaacatggcc gccgacagat tatttcactt ctttaaaacc agcggctttc
    17281 atcaccagtt ccatttgcgc catagtgata ccttttttgg catcttcagc agaaacgttg
    17341 attcctgaaa taccctgcag ggctttaaaa tccacttttt ccatatcgat agtcacgttt
    17401 tcctgcgcgt aggtatctgt ataggttaat ttttcttcaa cacccgcgat gtttttgtat
    17461 ttggcgctta acggctcaag tgtcttggca gcgtcttctt tggtggttgc accaatagaa
    17521 gcaaattgaa ttttggtttc agaagattgc ttaagcacct tgtcaccttt gtagacatag
    17581 gtaatggcaa tttcagtgcc gttcagattg gcgctgaatt tcttcgattc ttctttgtca
    17641 ccgcagccag caagagagaa aaccagaaca gatgcaacaa cgagggaaaa cagcttattg
    17701 aaagccttca tgtaaaactc cattttattt aatcaagaaa ctggtgactc tcaccagggg
    17761 ctatatagaa tatgcctaat accgtgacgt gagcagtccg gaactggagt agaactctta
    17821 gtaaaaagca ctatttcatc cttgttgctg aagcatgggg aataattgtt cgcaaagtaa
    17881 aacaccgtta ttcattgctt ctacccgtgc ctcgctttct gtattacgaa attgtcccaa
    17941 cacatgtgcc agccgataaa aaccgaccgc ggagaggtca ttcgccagca actctgcctg
    18001 actaatagcg ctctgttcct gatagcgcca gccgttatgg agcagttgaa taagtaacgc
    18061 ctggcagcgc atcagcaact gatgagcggt agacggcaca ggcaaaacgc tggcagaagg
    18121 tagcggtgcc acaggcgcag tttctgcgtc cagcgcccag gcgcgggttt ttgtcatcat
    18181 cacccgtggt tccagtgtca attgcccatc aacaaaactg acaaagccag aaaccagact
    18241 cacggggtcg tctgtttgtt gcaaaagcgc cgccatgcgt tcaacggcaa aaggagaaca
    18301 ggcagatgcc ggaagggata acgtcaggag attatcttca ccttcgccgc tgattacctg
    18361 cgcatccagc gtctggcggc tgctatccca accgagcgaa atacactcag cgaccggcag
    18421 aataaataag ttatcgacct gattaagagg ccgtatgcag gcggggggac gctggcgtaa
    18481 atattcccgc aaagccacaa tgcccggctg gcgtaacggc gcgctcaaca tttgccaggc
    18541 atcaggcgac agcggcacaa cgctgcttaa gcggttgcgg gtagctaaca gcagctcgcc
    18601 atcggcactg cgttttgctg cttgtgaaac aatttgccca cccgccagtg cgccagcctg
    18661 aaaactaaac agccgacgcg tagctgccgg tgagttttcc tgttcacttc gcggccaact
    18721 gcgcgaaagg tgcaaaatac tgccggtgtc gggatcggta aaccagatgc gtaaaccata
    18781 atgctcaata tcctgccagc aacgcatacc taaagacacc agccgcagat gatcaagctt
    18841 tgcttctccg gcaatgccag agccaacgac cgtgcgccac ggcacaggag gaacttcacc
    18901 aacactgtcg cgccgggcca tctcttgtgc gcaatttaat cgactgttta atgccgcaag
    18961 ctgacgtaag cattctacgg catgatagtg gctggcgcga gtgtggaagg catcaacgct
    19021 tgcccgtagc tgtcgtagtg attcactcac ccagcgccag ttgcaggcct ctgccgcctg
    19081 caatgtgcgg ttgaatgctg cctcgtagtg aataagcggc tggctgatgc cgccaagcca
    19141 taatgcctgg cttaattgct gaacatattg acgacacgtt tggccttctt cgctggcaaa
    19201 cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc cagattaaat ggttaaattc
    19261 tgcttgctgc gctttggcct cgacgaaggc ctgcacagcc agtacgacat gttcgcaaag
    19321 tgtgccttca atacaatcac aacgggcgaa acgaatactg ctacgggaat aaaaacgcac
    19381 atcgctcatc ggtaagcggg cagagggaat ttcgcccggc gcacagaaca actcaatggt
    19441 gatgccttta gcgaccagcg cctgcgcgcg tttgcgggtg gcatcgggca gggtagccag
    19501 ttcttccagc cagattgccg gatcccactc ttcttctttt tccgtgggct ggacggtggc
    19561 acaaagtcgt tgataactta acaccagcat cacgcgatga cggcacatgc cgctggcccc
    19621 gcagctgcac tgagcctctt tcagtgcctg gctgttcgcc agctgggtac ggacaccgtc
    19681 actgaaggtg gcgattaaag cgccgttctc atggctgatt tccgggacgt tgccattttc
    19741 cagttcctta agactgcgct taacaaaacc ggcattgctt aacgccgtca gtgcctgcgg
    19801 tgtcagttct aataattccg gacgtagtga attcatgact gaagattctc cgcaagccat
    19861 gatgccagct cgcccggcgt catggcggct atttgtgcgc cgacattaac cagcgcctgg
    19921 gccgtatcgc ggtcatagca aggcgttgcg gtgctatcga gcgctgccag tcccagcact
    19981 ttgatgccgc tctggacaca ctttttcacc tgatgcgtca gcaatgatga tgaaccccct
    20041 tcgtaaaaat cgctcacgag gataatgacg ctttttgctg gttgttcaat aagttgccga
    20101 ccatactcca cggcactggc gatattggtc ccgccgccca actgtacttt cattaataac
    20161 tctaccggat cggcaacgtc tgccgtgaga tcaaccacgc ttgtgtcaaa cgccaccaga
    20221 tgggtacgaa tgccgggtaa ctgccacaaa caggccgcca tcaccgcaga gtggatcacc
    20281 gagtccacca tcgatccgct ttgatcaacc agtaagacca gttgccattg ttcgctttgg
    20341 cgtttaatgc ggctgttaaa gcggggggat tcgatataca acttgccgtg ttgcgggtgc
    20401 cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa agtttcgcgc cagtggaatt
    20461 aatgagcggc gacggcgatc gcggacacca gaaaaagcct gacgaacttc ctttgccagt
    20521 cgcgccataa tttcttcaac aacctggcgc actatctggc gggcggcagc cagtacttcg
    20581 ggattcatca gatgtttggt gtgcaaaacg gcgcgtagca ggctttccga aggctgcata
    20641 cgttccagca cgtcgagatt cgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg
    20701 ctttccagtc gctcaatcac ctgttgcgga aacagagtgt gaatactgtt gatccactca
    20761 ggggtggtga gatttgagcc acctaatcca ccagagcgtt caccacgctg gagccgttca
    20821 ggatcgcgcc catacagcca ctccagcgcg tggtctatct gccgggcgtt gtcatccagc
    20881 ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc gccagcgttg tagctcacgg
    20941 gtggtcagaa gatcgttcag ttcagacatg gaaaactccc cagttttgca gcgatgccag
    21001 tgcctgctgt tcgagttgtt gatgatgtgc aatggcctgt ggtggacaat gcaacggcat
    21061 ttgcagcgcc gaaacgggaa gttgcgccag ttgataatgc tcaagcacct gatgcgccag
    21121 cgtcccgcgt tctcgtggtg gtagccaggc catcgccgcg cgtaaatcgg gcagggcatt
    21181 gataaaatcg gcatcactta gttgatttaa atgactgctg aaaccggcga taaatgtcgg
    21241 ctgacaggcc agttgatggc gggccagcgc cagcaaaccg tgcagcgctt cgccggattg
    21301 tgccggggat aactgcgcca gcatcgtcag cgccgcttcg gcactggcgt taggatgttc
    21361 cagacgcatc agtgcgccat gagccgcgcc gcgatccagc gccggggcat gaattgcctg
    21421 actgcgtcgc tccagtaagg ctaccgccgc agaaagagaa atgccgggga gttgcacacc
    21481 gctatgtaga tcgcgcagga tatgacacaa cgcttgccag ctatgcaggt gagcgtgaaa
    21541 ctccttttca tccggtctgc cgttagattc acacagccac agcgtgcgat cgatagccgc
    21601 gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg ctaatttcat ccagccgcca
    21661 taaggcatat agcacttcca gcgcctggcc catttcggca aattgatttt cctgggcgat
    21721 taataatgtg agttgctcca gtaattgttg actgaaggac gccagacccg ctaacgccgc
    21781 ctggctaaga catgtggtga tactgccgat tccacccgcg tccagcatat cggcttctaa
    21841 tttatggcgt gcggcttcct gaagtgttgc accaaagcat gcggcctcaa tcaatgccgc
    21901 atgctggcta agcgggcggg ttaatttcca gtgttcttca ccgttgcctg caagtgtcag
    21961 tgtacttccc tgctggcgta caatcccagg gatctccagt attgccagcc gatgtaacac
    22021 ctgactttgc gccagcccat cgggggtaaa gcgatttaag gtaagctccg ccggaagaga
    22081 gattgcggta cgttccagtt cgcaggtgac atcttttggc agaggcggtt gtggcgtgga
    22141 aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg
    22201 atcggtatcg ggatgaataa cgccgcgata gctccacggc aacggtgcat tcagggcttc
    22261 tttaatcaac gagcctgcta tggcatccag ccagtcagtg cgtaacggta gtgtatgacc
    22321 gcgcaactgt gccagtgcca ttgcatgcag atgtgcggca gccatatccg cggtcgaagc
    22381 aggcaagttg tgctggcgca aacgggtgag aaccgttttt agcagttgtt caccggcctg
    22441 ctgtaagccc cactgccagc accagttttg ccagaccggg gcaggcatac cggaaaggta
    22501 tcctgccagc acatcaaggc gcttttcact gtagggcgtg agataacaac ctgtaatggc
    22561 atctgccagc gagggcaatt ccggcgtgtt aatgtcctgc gggcattcgc gccacatttt
    22621 tgccagtgcc ggagcgtgcc agccaccgca gacgactaac acgtcgccat tattttgctg
    22681 caccgcccat gcaatccagc gagccataaa ggcttcgcgc tgacgattga gtgcatcgcc
    22741 ggggaaatcg ccccgcagtt gggcaaaata gtgcgccagc gcagagggta atgcagtttg
    22801 ctggctttca tcttcgaaca agtgatccca cagggtatcg ctgttatcca tgcgggtggc
    22861 acgcagcagt aaggcctggc tttcatcttg cgtatcaggc gaatcgtcct cttcttcact
    22921 ctgcgcccag caaggcaaat cgatgaagta agtttgtgcc tgaatacgac gtgcggcttg
    22981 tagcgcctgc cactccggcg aaaattcagc aaatggcgtc caggcaccac gcccgggggc
    23041 tgcaccgtcc tgatactggc aataactgta aatagctacc ggaagctggt gggaaaaaaa
    23101 aagttcgtct acccggtcat taaaatcagc cgggccttca atcaacacgt atcgtggccg
    23161 ctggctttcg attaacgatt tcaccagccg ggcgcaggcc ggactatgat gccggatgcc
    23221 gacaattaac ggctcgctca tgcctctttc ctcacggcag gcggtggcgg gcttgataat
    23281 aagcctgcca gtgagcttct ttatgtgtgg cgacacgctg ttcgaagtaa cggcgcagac
    23341 gagcgcgatc ttcttcgtta tctttgacga tggttccggc aatacagtcc accaggtcgg
    23401 caggctcccc cgcgcgattc gctaaaaacc aggcgcggac gcctacggcg tgggccacgt
    23461 tcacggcttc agcggtggac ataatcgctg tcagtgtatc cattgacgtt ttcttttcgc
    23521 cgttggcacg cagatcgcgg aaggtgcgta ccagtaactc cagtactgcg tcaggcactt
    23581 tatgcggaat accgctatgg gccagcaaac gcgccgaagc actggcgacc agttccagct
    23641 cctgggcgaa atccataatc ggaaagacgg tttcaaaatc gaagcgacgc tttagcgcgg
    23701 cactcatctc attgacgccg cgatcgcggg tattggcagt ggcaataata ttaaagcctt
    23761 cccgcgcata gagctgactg gcttcaccgg tgagttccgg caccgtcatc accctgtcag
    23821 aaagcatacc taacagacag tcttgtactt ccagcggtgt acgcgttatc tcttcaaaac
    23881 ggacgatttt gccgtcgcgc attccctggt aaagcggggc agggaccagt gcttccgttg
    23941 acggaccatg gttgataagc agggcgtagt tccagccgta cttgatttga tcttcggtag
    24001 tagatgcgcc gccctggatg gttaacccgg catcgccgct aatagcggtt gccagtaatt
    24061 cagacaggag agattttgcg gtgccaggtt cccccaccaa catcaaaccg cgcccggtgg
    24121 caagagtgac taacatgcgt tcgacagcgg agggactaat caccacttta cggctaatat
    24181 tttgcgcgct atcgccaagg ataaaagcac gggccgcagg caaacttaac tgccaaccgg
    24241 gcgggcaagg tgcgttatca ttttgtttta attttgccag ttcatcggcg tataacacag
    24301 cagcaggcgg acgctgcaga tgattattct gcggtgacat atattcatcc ctgaaatgta
    24361 ttaatgaaat aaacgatatt ttactcgcaa taaatgtttg gcgcattagt tgtttgagcg
    24421 aaaaatatct ggaaagcaga tcacagtaaa taaaaaaacc tgctggtgag gcaggttctt
    24481 ttatttacta ctttcaggcc taacaccaca gcctgtgcaa catcgtgttc caacgaacga
    24541 attataacca cggctggacc ccgttgcaat taagataatg agatttataa tccagaccgt
    24601 tgggacgttg cgccggatcg aatctatttc aagtcctggc taccggacgg gtggtgctgc
    24661 aggtggtgcc ctgatccgga gtgcaacatc gtggtccaac atacgtaaac cagtatcgac
    24721 aggcagtgta agggtaaggt gctggtgttg cgctccttaa taaggagagc ggatggacac
    24781 gctgacacaa aagttaaccg tgctcattgc tgtactggag ctattagtgg ctctgttacg
    24841 gttgattgat ttgttgaagt aacgggcaaa ttgaaacgca tctaaggggg aaaaccgctt
    24901 ccccctttaa taaaatgatt actgttttta ttaattcatc atataactca tttttattta
    24961 taaattcatc gattttatat tcaacgcacc atttgaccgg gatgtgaata tttatggtgg
    25021 gctattggca aaaaatatct ggaaagtaga tcgcagaaaa taaaaaaacc tgctggtgag
    25081 gcaggttctt ttatttacta ctttgatgtt aagttactga tagtgcaaca tggcatctca
    25141 acgaacgaat tataaccacc tctggacagt gttgcaatta agataatgag atttataatc
    25201 ctgactgttg ggaagttgcg ccggatcgaa cccatttcaa gtcctggcta ccggacgggt
    25261 ggtgccgcag gtagtgctct gatccggagt gcaacatggc gactcaacaa acgtaaacca
    25321 gtatcgacag gcagtgtaag ggtaaggtgc tggtgttgcg ctccttaata aggagagcgg
    25381 atggacacgc tgacacaaaa g
//
LOCUS       DAZFFI010000066        24803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFFI010000066 DAZFFI010000000
VERSION     DAZFFI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.066"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2688
                     /gene="ehaG"
                     /locus_tag="ACHJ12_003089"
     CDS             <1..2688
                     /gene="ehaG"
                     /locus_tag="ACHJ12_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4990842.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVA
                     KGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLA
                     VGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTT
                     TRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQ
                     VSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSV
                     AIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLK
                     SSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQE
                     TKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGES
                     AVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3057..4712
                     /gene="lldP"
                     /locus_tag="ACHJ12_003090"
     CDS             3057..4712
                     /gene="lldP"
                     /locus_tag="ACHJ12_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4990843.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4712..5488
                     /gene="lldR"
                     /locus_tag="ACHJ12_003091"
     CDS             4712..5488
                     /gene="lldR"
                     /locus_tag="ACHJ12_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4990844.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            5485..6675
                     /gene="lldD"
                     /locus_tag="ACHJ12_003092"
     CDS             5485..6675
                     /gene="lldD"
                     /locus_tag="ACHJ12_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4990845.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            6861..7334
                     /gene="trmL"
                     /locus_tag="ACHJ12_003093"
     CDS             6861..7334
                     /gene="trmL"
                     /locus_tag="ACHJ12_003093"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4990846.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7387..8208)
                     /gene="cysE"
                     /locus_tag="ACHJ12_003094"
     CDS             complement(7387..8208)
                     /gene="cysE"
                     /locus_tag="ACHJ12_003094"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4990847.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8288..9307)
                     /gene="gpsA"
                     /locus_tag="ACHJ12_003095"
     CDS             complement(8288..9307)
                     /gene="gpsA"
                     /locus_tag="ACHJ12_003095"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4990848.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9307..9774)
                     /gene="secB"
                     /locus_tag="ACHJ12_003096"
     CDS             complement(9307..9774)
                     /gene="secB"
                     /locus_tag="ACHJ12_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4990849.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9837..10088)
                     /gene="grxC"
                     /locus_tag="ACHJ12_003097"
     CDS             complement(9837..10088)
                     /gene="grxC"
                     /locus_tag="ACHJ12_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4990850.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10230..10661)
                     /locus_tag="ACHJ12_003098"
     CDS             complement(10230..10661)
                     /locus_tag="ACHJ12_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4990851.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10906..12450
                     /gene="gpmM"
                     /locus_tag="ACHJ12_003099"
                     /gene_synonym="pgmI"
     CDS             10906..12450
                     /gene="gpmM"
                     /locus_tag="ACHJ12_003099"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4990852.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12484..13743
                     /gene="envC"
                     /locus_tag="ACHJ12_003100"
     CDS             12484..13743
                     /gene="envC"
                     /locus_tag="ACHJ12_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4990853.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13747..14706
                     /locus_tag="ACHJ12_003101"
     CDS             13747..14706
                     /locus_tag="ACHJ12_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4990854.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(14693..15727)
                     /gene="waaH"
                     /locus_tag="ACHJ12_003102"
     CDS             complement(14693..15727)
                     /gene="waaH"
                     /locus_tag="ACHJ12_003102"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO4990855.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRHDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15966..16991)
                     /gene="tdh"
                     /locus_tag="ACHJ12_003103"
     CDS             complement(15966..16991)
                     /gene="tdh"
                     /locus_tag="ACHJ12_003103"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4990856.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDQASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17001..18197)
                     /gene="kbl"
                     /locus_tag="ACHJ12_003104"
     CDS             complement(17001..18197)
                     /gene="kbl"
                     /locus_tag="ACHJ12_003104"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4990857.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVIEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18472..19329)
                     /gene="yibB"
                     /locus_tag="ACHJ12_003105"
     CDS             complement(18472..19329)
                     /gene="yibB"
                     /locus_tag="ACHJ12_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO4990858.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            19632..20564
                     /gene="rfaD"
                     /locus_tag="ACHJ12_003106"
     CDS             19632..20564
                     /gene="rfaD"
                     /locus_tag="ACHJ12_003106"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4990859.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            20574..21620
                     /gene="rfaF"
                     /locus_tag="ACHJ12_003107"
     CDS             20574..21620
                     /gene="rfaF"
                     /locus_tag="ACHJ12_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4990860.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            21624..22616
                     /gene="rfaC"
                     /locus_tag="ACHJ12_003108"
     CDS             21624..22616
                     /gene="rfaC"
                     /locus_tag="ACHJ12_003108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4990861.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNFEIITNKLTSEIR"
     gene            22613..23821
                     /gene="rfaL"
                     /locus_tag="ACHJ12_003109"
     CDS             22613..23821
                     /gene="rfaL"
                     /locus_tag="ACHJ12_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO4990862.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(23857..>24803)
                     /locus_tag="ACHJ12_003110"
     CDS             complement(23857..>24803)
                     /locus_tag="ACHJ12_003110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4990863.1"
                     /translation="RIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPN
                     GIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLK
                     LVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPS
                     QFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTL
                     ANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWLE"
BASE COUNT         6268 a         6300 c         6163 g         6072 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg gggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tccaagaaaa cggtgcgggt
     1081 attaactatg tgcgtactaa cgacgacggc ttagcgttca acgacgccag cgcacagggt
     1141 gttggcgcta cagctatagg ttataactct gtcgccaaag gcgatagcag cgtagctatt
     1201 ggtcagggca gctacagcga cgttgatacg ggtatcgccc tgggtagcag ctctgtttcc
     1261 agccgagtga ttgccaaagg ctcccgtgac accagcataa cggaaaatgg cgttgttatt
     1321 ggttacgaca ccacggatgg cgaactgctc ggtgcattgt ctatcggtga tgacggtaaa
     1381 tatcgtcaaa tcatcaacgt agccgatggt tccgaagccc atgacgccgt tacggttcgt
     1441 caattgcaga atgcgattgg tgcggtcgca accacgccga ctaaatactt ccacgctaat
     1501 tcaacggaag aagattcact ggcagtggga actgactcgc tggcaatggg tgcgaaaacc
     1561 atcgtgaatg gcgataaagg tattggtatc ggttatggtg cctacgtgga cgcgaatgca
     1621 cttaacggca ttgccattgg tagcaatgcg caagtcattc atgtcaacag tattgcgata
     1681 ggtaatggtt ctacgaccac tcgtggcgct caaaccaatt ataccgccta caacatggac
     1741 gcaccgcaga actctgtcgg tgaattctca gtcggtagtg cggatggtca acgtcagatc
     1801 actaacgtcg cagcaggttc ggctgatacc gatgcggtca acgtgggtca gttgaaagta
     1861 acggatgcgc aggtttccca gaatacccag agcattacta acctggataa tcgggtaacg
     1921 aatcttgatt cacgcgtcac caatatcgaa aacggtattg gcgatatcgt caccaccggt
     1981 agcaccaagt acttcaagac caataccgat ggtgtagatg ccagcgcgca gggtaaagat
     2041 agcgtcgcga ttggttctgg ctccattgct gccgcggata acagcgtcgc actgggtacc
     2101 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     2161 atcaccaacg tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     2221 tcttctgaag caggcggcgt gcgttacgac accaaagctg atggttctat cgactatagc
     2281 aatatcaccc tcggtggcgg caacggcggt acgactcgta tcagcaacgt ctccgctggc
     2341 gtcaacaaca acgacgcggt gaattacgcg cagttgaagc aaagcgtgca ggaaacgaag
     2401 caatacaccg atcagcgaat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     2461 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     2521 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     2581 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     2641 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     2701 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     2761 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc tttttcttta
     2821 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
     2881 tcacaattcc ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca
     2941 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
     3001 aaggagatga gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga
     3061 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
     3121 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
     3181 ccgcctcgtg gacggtggca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
     3241 tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
     3301 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
     3361 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
     3421 gtttctgttt cggcgctttc cttgaaggag ccgccggctt tggcgcaccg gtagcaatta
     3481 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
     3541 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
     3601 aagtaacagg catcgacagt tttgagattg gccagatggt ggggcgtcag ctaccgttta
     3661 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
     3721 agacgtggcc tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct
     3781 ccaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
     3841 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
     3901 cgtcacaggt tgatatgacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
     3961 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
     4021 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
     4081 acctcgataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
     4141 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
     4201 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
     4261 tgaaagaact ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
     4321 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
     4381 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
     4441 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
     4501 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
     4561 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
     4621 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
     4681 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
     4741 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
     4801 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
     4861 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
     4921 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
     4981 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
     5041 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
     5101 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
     5161 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
     5221 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
     5281 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
     5341 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
     5401 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcatta
     5461 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
     5521 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
     5581 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
     5641 aaaaacatgt ctgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
     5701 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
     5761 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
     5821 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
     5881 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
     5941 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
     6001 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
     6061 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
     6121 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
     6181 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
     6241 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
     6301 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
     6361 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
     6421 cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tacttctggg tcgtgctttc
     6481 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgag
     6541 aaagagatga aagtggcaat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
     6601 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
     6661 gggaatgcgg catagtcgtt tgagccccct caccctaacc ctctccctca gggagtgggg
     6721 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
     6781 ctcgcccctc cggggtgagg ggaaaaggcc gcactgctca ccaattttct gctatcctgc
     6841 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
     6901 atactggcaa tatcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
     6961 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
     7021 ttaccgccgt tacgcgtcat catgactttc gcgcattcct cgaagcagaa aatccccagc
     7081 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaaac tatcaggatg
     7141 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
     7201 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaacctgt
     7261 ccaatgcggt gtcagttgtg gtgtatgaag cctggaggca gctgggatat ccgggagcgt
     7321 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
     7381 aggacatcag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
     7441 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
     7501 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
     7561 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
     7621 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
     7681 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
     7741 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
     7801 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
     7861 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
     7921 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
     7981 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
     8041 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
     8101 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
     8161 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
     8221 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
     8281 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
     8341 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
     8401 caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
     8461 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
     8521 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgcca agccccgcca
     8581 tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac atttcagcca
     8641 gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
     8701 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
     8761 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
     8821 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
     8881 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
     8941 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
     9001 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
     9061 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
     9121 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
     9181 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
     9241 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
     9301 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
     9361 catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg taccgcggga
     9421 taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
     9481 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
     9541 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
     9601 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
     9661 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
     9721 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
     9781 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
     9841 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
     9901 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
     9961 tcttcacgct tggcggcatt gccatcgatc ggcagctcct ggaaactcac gcccttgctg
    10021 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    10081 ttggccataa attagctcct gtttttttac cctgaaattt catgttgcag ggcggcagca
    10141 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    10201 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    10261 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    10321 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    10381 tctttgtgct tctcaagctc accaacattg ttggctttga tttcactcgg caacagattg
    10441 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    10501 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    10561 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    10621 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    10681 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    10741 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    10801 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    10861 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    10921 aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca gcaggataac
    10981 gccattttta gtgctaaaac gccggtaatg gatgcactgt gggccaatcg tccgcatacc
    11041 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    11101 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    11161 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    11221 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgtagg cggcgtacac
    11281 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    11341 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    11401 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    11461 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    11521 ctgctaactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg tttgcaggca
    11581 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    11641 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    11701 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    11761 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    11821 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    11881 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    11941 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    12001 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    12061 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    12121 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    12181 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    12241 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    12301 ctgccagttc cgctgattta tgttggtgat aagaacgtga aagcggttga aggcggcaaa
    12361 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    12421 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    12481 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    12541 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    12601 ctcaaatcta ttcaggccga tatcgccgca aaagagcgcg cggtacgcca aaagcaacaa
    12661 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    12721 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    12781 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgcg
    12841 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    12901 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    12961 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    13021 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    13081 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    13141 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagc
    13201 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggcccag
    13261 gcggttcgcg accgccagaa agaagcgacg cgcaaaggca ccacctataa accgaccgaa
    13321 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    13381 tggccggttc gcgggccgac gctgcatcgc tatggtgaac agctacaggg cgaactacgc
    13441 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcgat tgccgacggt
    13501 cgggtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt tgagcacggt
    13561 aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt tggttcgcag
    13621 gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg tcggccttca
    13681 ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg gttgggaaga
    13741 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    13801 tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgcac
    13861 aacgaaaacc aggtgctggc gatgccctcc gctatctccg tcgctgtatt acccgattca
    13921 ccgcacgcca gagagatggc gaccaaagcg cataacagcg ggcacgaagt gttgattcat
    13981 ctcccgatgg caccactgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    14041 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    14101 gggatcaaca accacatggg tagcaagatg acctctaacc tgtttggtat gcagaaagtg
    14161 atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat cggtaatacc
    14221 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtattcctc
    14281 gacgattcgc aaaatgaagc ggacatccgt gtgcaattta atcgcgcaat tgatctggcg
    14341 cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgcta
    14401 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    14461 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    14521 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    14581 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    14641 cagcatcagt ggcaaggctg gggcaaacag cccgaagcgg caaaacttaa cgctagcgca
    14701 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    14761 agcggaaaga ccacagtaac gcctggtaac ccaccttcac gctgcgcaca ttggtaatca
    14821 ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg gtaagaatat
    14881 ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt tgctgatgaa
    14941 attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc agcaggcggg
    15001 taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta tgcaaccgac
    15061 tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat cgcgcacgca
    15121 gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat ccggcaataa
    15181 atttaatgtt atttttaaca ataacatcat ggcgataaac ccccatccag acaacgtgag
    15241 tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat acgccggttg
    15301 agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga aaacaccagt
    15361 cagcgttgca ctgcgccacg tcgaggtcat cctctaacgc catggtcatc agcgtttcgt
    15421 acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg cccgttgcca
    15481 cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc aacaaacgaa
    15541 cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc gttgaaccat
    15601 cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat tccatacaag
    15661 tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta ttggtgctgt
    15721 tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc tttgtattaa
    15781 gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt cagggtagag
    15841 agataatgag agcagctata taagagatca gcgcaattac gatatcttta ttacactgac
    15901 aggattttac atatttaacg gattatcctc aggcaaaagg gctggaatac cagcccttgt
    15961 tcgtgttaat cccaactcag aattactttc ccggactggc ccgaacgcat agcgtcaaag
    16021 cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag atccagacct
    16081 gactgaatca gcgccgccat cttgtaccag gtttcaaaca tctcacgacc gtaaatacct
    16141 ttaatgaaca agcctttaaa gattactttg gtccagtcga tagacatatc ggacggtgga
    16201 atacccagca tcgcaatgcg accgccgtgg ttcatggtgt cgagcatggt acgaaacgct
    16261 ggcggcgcac cggacatttc cagaccgaca tcaaagcctt cggtcatgcc cagttctgcc
    16321 atcacatcat taaggttttc tttagcgacg ttgaccgcac gggtgatacc cattttgcgc
    16381 gccagttcga gacgatattc attaacatca gtgatcacca cattgcgtgc accaacgtgt
    16441 ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagagaccag cacatcctcg
    16501 ccaaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc aaaaatggaa
    16561 gcctggtcgt cggagatatt gtctgggatt ttgaaggcgt tgaacgccgg gatcaccaga
    16621 tattcggcga agcagcccgg acggttaacg ccgacgccga tcgtgttgcg gcacagatgg
    16681 gtacgaccgc cacgacagtt gcgacagtga ccgcaagtga tatggccttc gccagaaacg
    16741 cgatcgccaa tcttgaagcc tttcacttcc tgaccaatac ctaccacttc accgacatat
    16801 tcatggccca cgaccatcgg caccgggatg gttttttgcg accactcatc ccagttatag
    16861 atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag atcgttatga
    16921 cccagttccg gtacaggaac gtcggtcatc cagatgccct cttccgcttt cagtttggat
    16981 aacgctttca tctcacatcc tcaggcaata acgcccagtt gtttaccaat acgcgtgaat
    17041 gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg ggtacgaata
    17101 cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtaaat gccctctttt
    17161 tgcagctcac gggcaaattt ctgcgccact accgcatcgc caagcatgac cggaataatg
    17221 gcatgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg gaactgacgc
    17281 gcgttcgccc acagacggtc acgcagctcg ctacccgctt caaccatctc cagcactttg
    17341 atagaagcgg caacaatggc cggagccagc gagttggaga acaggtacgg acgagaacgc
    17401 tggcgcagcc actcaatcac ttctttgcgc gccgcggtat aaccaccaga agccccgccc
    17461 agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc ccatcacatc gcagtattca
    17521 tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc taccatcacc
    17581 agggcatcgt atttatctgc cagatcgcaa acgcccttca ggttggcaat cacgccgtcc
    17641 atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc acgcgcttct
    17701 ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg tttagcttta
    17761 cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat aatggcgtct
    17821 tccgcaccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga agagtagaga
    17881 atcgcatctt ccatccccag gaaagccgcc agtttttgtt caagctcttt atggctgtcc
    17941 tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc cattcccgcc
    18001 tttgccgccg caatcagatc aggatgattc gccagcccga gatagttgtt ggcacaaaag
    18061 ttaatgacgt ggcttccatc agccacagtg atatctgctt gctgcgcaga cgtaataatg
    18121 cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt taactgctga
    18181 taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca tattacccaa
    18241 acttataggt cgcgacgaga tgacgcgtta acacttctgc aaaattcagg ataaataacg
    18301 tgtaacttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt tttggctggg
    18361 cataatgatg attgatttta ctgcattgat ataaaaagaa aaaatgatgc caaagcatca
    18421 tttttcctat ctcagttggt atcaaaacgc tacccactaa tggagggaaa cctattttct
    18481 tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc tgaagcagcg
    18541 aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat cggggcgttt
    18601 gtaatagcac atgacaaaaa tgccctggtc atcatccaca atgttattat ttagtgttat
    18661 tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg aaccaacaat
    18721 tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaaacct gttgttgaga
    18781 tgtgacagta agcccttttt ttatagtgaa aagatgcatc ttactttcat caaatgggaa
    18841 atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa aatctatcca
    18901 cgcaacaaga ggcgttttca ctaagcccat gttgatagct ttatttacaa aataggcctt
    18961 aaggttacat accaaaacat actctggtga ccagtactct gggtttttta attgtcgagg
    19021 ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac ttctgatata
    19081 tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt tgcgaatcgc
    19141 ttcaaccctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt caagtgcggc
    19201 cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt cacggaaccc
    19261 tttattagca gtccaatccc ctctaccaat gtcaaaatat gcagtgataa ttgtggtgga
    19321 tgatttcatt actaataaga ccattaatat aaaaaagaat tatatcataa atattaacag
    19381 aatgtatact gtatattatc cattcaaatg cttccgttat ttatcataca tcggaatatt
    19441 gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact agctaaaacc
    19501 caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg aagggattcg
    19561 gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc cataattcga
    19621 aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc aacatcgtta
    19681 aagccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg aaagacggca
    19741 ccaagtttgt gaacctggtg gatctgaata tcgcagacta tatggataag gaagacttcc
    19801 tgatccagat tatggctggc gaagagttcg gcgatgtcga agcgattttc cacgaaggcg
    19861 cgtgctcttc caccaccgag tgggacggca agtatatgat ggataacaac tatcaatact
    19921 ccaaggagct gctgcactac tgcctggagc gtgaaatccc gttcctgtac gcttcttccg
    19981 cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac gaaaaaccgt
    20041 tgaacgtcta cggttactca aaattcctgt ttgatgaata tgttcgccaa atcctgccgg
    20101 aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc
    20161 ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat aacggtgaat
    20221 caccgaagct gttcgaaggt agcgagaact ttaaacgcga cttcgtctat gttggcgatg
    20281 tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggcatcttc aatctcggca
    20341 ccggtcgtgc ggaatccttc caggctgtag ctgatgctac gctggcttat cacaagaaag
    20401 gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag gcgttcactc
    20461 aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa accgttgctg
    20521 aaggtgtaat ggaatacatg gcctggctga atcgcgacgc ataagagctc tgcatgaaaa
    20581 tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt ctctatcgca
    20641 cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca tggtgccgtc
    20701 cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc ggtcacggag
    20761 cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc
    20821 gcgcctacgt cttaccaaac tccttcaaat ctgcattagt gcctttcttc gcgggtattc
    20881 ctcatcgcac tggctggcgc ggcgagatgc gctacggttt actcaacgat gtacgcgtgc
    20941 tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag
    21001 gcattatgcg taccgcacaa gatctgccgc agccattgtt atggccgcag ttgcaggtga
    21061 gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa cgtccgatga
    21121 ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac taccactatg
    21181 cggagctggc aaagcagctg attgatgaag gttatcaggt ggttctgttt ggctctgcga
    21241 aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag caggcatggt
    21301 gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt gcagcctgta
    21361 aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc
    21421 tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc cataaagcgc
    21481 gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt
    21541 atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc aacgcgctat
    21601 tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg
    21661 atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca gggattaagt
    21721 ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct gccgttgagc
    21781 gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc cccataaaag
    21841 ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca gttatcgacg
    21901 ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc gtaaagcatg
    21961 gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat tgtaagcatc
    22021 atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc aaaagtttgg
    22081 gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt ctgacgaacc
    22141 tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt gatgataaac
    22201 actggccgga agaacactgg cgagaattga ttggtttact ggctgattca ggaatacgga
    22261 ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg gcggaaggat
    22321 ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc gtgctggccg
    22381 gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg gcactggata
    22441 gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg tatgggaaga
    22501 atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct caaaccgttt
    22561 ttcaaaattt aaattttgaa ataataacca ataagttgac atcggagata agatgacctc
    22621 aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca gagctctcgt
    22681 attcttattc attaccacct attttttggg tgggattaca aggtataaac atcttattgt
    22741 tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac actatctctc
    22801 actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg cattgctgtc
    22861 acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag ctattattga
    22921 gaatacttta ctatgcacaa tagcaatacc gatcatattg agagacgaga aaagagaaga
    22981 tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct gtttttctga
    23041 attgattacc tattataagg actatcaaca agggataatg ccattcgcag attatagaca
    23101 ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc tctggcttat
    23161 caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta tttttctgat
    23221 attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat taatatggat
    23281 tctgatgttt aaacaatgga agttactatt agtaggagta atggttgcca tcattgcatt
    23341 gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac ttcaacaaac
    23401 taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc taatattaga
    23461 aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct ataataaacg
    23521 tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata attttgcgct
    23581 attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc tatactgtgc
    23641 aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac catataatgc
    23701 atattatata atcttactat cttttatagg ttattttgtt atccgtggaa acgtagaaca
    23761 aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga aaaacaagta
    23821 aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaagccaat tatgaataac
    23881 ctcttcgaat ctttgagtta cgccatccca actgtagtgt tcaaacacaa agtcctgacc
    23941 ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat cgctgcttat
    24001 ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt tgacaaattc
    24061 cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag cttcaattgc
    24121 caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg ccagtgggta
    24181 ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat ccttcaaacg
    24241 ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac tcatttctgt
    24301 ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt tttcgaatga
    24361 ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa aaataatttt
    24421 ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag attgataggt
    24481 ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat aagattggta
    24541 atactttttc aagtataaac taggcactat cattttaaca ttttgctcaa gcaattttgg
    24601 ttcaaatgca ttatgcatat gtataaccac tcttgcctga ggagctcgct tacgaatttg
    24661 agtgtatagc ttcatgctat tatgaactac aataacactt tctttcgtta taggaaaatc
    24721 atgtgcaata ttcaatattc tctgtgaata tggaagagga tcaagtcttg tccatttttg
    24781 aaataaccgc ttatatatgc gac
//
LOCUS       DAZFFI010000067        24424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFFI010000067 DAZFFI010000000
VERSION     DAZFFI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.067"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..363
                     /locus_tag="ACHJ12_003111"
     CDS             <1..363
                     /locus_tag="ACHJ12_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4990864.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHJ12_003112"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHJ12_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4990865.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHJ12_003113"
     CDS             2185..3504
                     /locus_tag="ACHJ12_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4990866.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTIFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3876..5258
                     /gene="dosC"
                     /locus_tag="ACHJ12_003114"
     CDS             3876..5258
                     /gene="dosC"
                     /locus_tag="ACHJ12_003114"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4990867.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5283..7682
                     /gene="dosP"
                     /locus_tag="ACHJ12_003115"
                     /gene_synonym="pdeO"
     CDS             5283..7682
                     /gene="dosP"
                     /locus_tag="ACHJ12_003115"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO4990868.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7940..8521
                     /gene="ddpX"
                     /locus_tag="ACHJ12_003116"
     CDS             7940..8521
                     /gene="ddpX"
                     /locus_tag="ACHJ12_003116"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4990869.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8535..10085
                     /locus_tag="ACHJ12_003117"
     CDS             8535..10085
                     /locus_tag="ACHJ12_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4990870.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQHKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10087..11109
                     /locus_tag="ACHJ12_003118"
     CDS             10087..11109
                     /locus_tag="ACHJ12_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4990871.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQRLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11106..12002
                     /gene="ddpC"
                     /locus_tag="ACHJ12_003119"
     CDS             11106..12002
                     /gene="ddpC"
                     /locus_tag="ACHJ12_003119"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4990872.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11999..12985
                     /locus_tag="ACHJ12_003120"
     CDS             11999..12985
                     /locus_tag="ACHJ12_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4990873.1"
                     /translation="MTQPVLDIQQLHLSLPGFNGDVHALNNVSLQINRSEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRQEARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12978..13904
                     /locus_tag="ACHJ12_003121"
     CDS             12978..13904
                     /locus_tag="ACHJ12_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4990874.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13960..14391)
                     /gene="osmC"
                     /locus_tag="ACHJ12_003122"
     CDS             complement(13960..14391)
                     /gene="osmC"
                     /locus_tag="ACHJ12_003122"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4990875.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14737..14952
                     /gene="bdm"
                     /locus_tag="ACHJ12_003123"
     CDS             14737..14952
                     /gene="bdm"
                     /locus_tag="ACHJ12_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4990876.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15054..15191
                     /gene="sra"
                     /locus_tag="ACHJ12_003124"
     CDS             15054..15191
                     /gene="sra"
                     /locus_tag="ACHJ12_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4990877.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15348..17045
                     /gene="maeA"
                     /locus_tag="ACHJ12_003125"
     CDS             15348..17045
                     /gene="maeA"
                     /locus_tag="ACHJ12_003125"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4990878.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17179..18189
                     /gene="adhP"
                     /locus_tag="ACHJ12_003126"
     CDS             17179..18189
                     /gene="adhP"
                     /locus_tag="ACHJ12_003126"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4990879.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18336..18609
                     /locus_tag="ACHJ12_003127"
                     /pseudo
     CDS             18336..18609
                     /locus_tag="ACHJ12_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(18660..19313)
                     /gene="fdnI"
                     /locus_tag="ACHJ12_003128"
     CDS             complement(18660..19313)
                     /gene="fdnI"
                     /locus_tag="ACHJ12_003128"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4990880.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19306..20190)
                     /gene="fdnH"
                     /locus_tag="ACHJ12_003129"
     CDS             complement(19306..20190)
                     /gene="fdnH"
                     /locus_tag="ACHJ12_003129"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4990881.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARDYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20203..23250)
                     /gene="fdnG"
                     /locus_tag="ACHJ12_003130"
     CDS             complement(20203..23250)
                     /gene="fdnG"
                     /locus_tag="ACHJ12_003130"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22663..22665),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4990882.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23482..24363
                     /gene="yddG"
                     /locus_tag="ACHJ12_003131"
     CDS             23482..24363
                     /gene="yddG"
                     /locus_tag="ACHJ12_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4990883.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5975 a         6208 c         6182 g         6059 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagcg ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgcact ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaattggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacga tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgtct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgccgaa gtggtttccc gctatccggt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac tgctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtaccgg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccgcaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgttttgatt
     3841 ccgtctgatt tcccagcctt ataagggtgt gaattatgga gatgtatttt aaaagaatga
     3901 aagatgagtg gaccggactt gtcgaacagg cagatcctct cattcgtgct aaagccgcag
     3961 aaattgccct tgcgcatgct cattatctga gtatcgagtt ttatcgaatt gtccgcatcg
     4021 acccgcatgc cgaagaattc ttgagtaatg aacaagttga gcggcagttg aaaagtgcga
     4081 tggaacgctg gattattaac gtgctttctg cccaggttga cgatgtcgaa aggctaatac
     4141 aaatccagca taccgtcgcg gaagtgcatg cccgcatagg aattccggta gaaattgtcg
     4201 agatggggtt tcgggtgctg aaaaaaatcc tctatccggt aatcttctct tcggattatt
     4261 ctgccgcaga aaaacttcag gtctaccatt tctcgattaa cagtattgat atcgctatgg
     4321 aagtgatgac ccgcgcgttt acctttagtg acagtagtgc ctcaaaggaa gatgaaaact
     4381 atcgtatctt ctcgttactg gaaaacgccg aagaagaaaa agaacggcaa atagcctcaa
     4441 tactttcatg ggaaatagat attatctata aaatcctgct ggattctgat ttaggcagta
     4501 gtttgccttt aagccaggct gattttggcc tgtggtttaa ccataaaggt cgacattatt
     4561 ttagcggcat tgccgaagta ggccatatct cccgtctgat tcaggatttc gacggtattt
     4621 tcaatcaaac catgcgtaac accaggaatt tgaataacag aagtttgcgg gtgaagtttc
     4681 tattacagat aagaaatacc gtatcgcaaa ttattacctt gctgcgtgaa ttgtttgaag
     4741 aagtatcgcg ccacgaagtc ggtatggatg tactgacgaa attacttaac cgccgtttcc
     4801 tgccgactat cttcaaacgc gaaattgccc atgccaaccg gaccggtaca ccgctgtcag
     4861 tgctgattat tgacgttgat aaattcaaag agatcaacga tacgtggggc cataacactg
     4921 gcgatgaaat tctgcgtaaa gtctctcagg ccttttatga caacgtccgc agtagtgatt
     4981 atgttttccg ctacgggggc gatgaattta tcattgtttt gactgaagct tctgaaaacg
     5041 aaacgttacg taccgcagaa cgtattcgca gtcgggtgga gaaaaccaaa ctgaaagccg
     5101 caaacggcga ggatattgcc ctctcacttt ccatcggtgc cgccatgttt aatggtcatc
     5161 ctgactatga gcgcctcatt caaatagccg atgaagctct gtatatcgcc aaaagacgag
     5221 gtagaaaccg tgttgaactc tggaaagcca gtctttagat gcgccaggat gcagaggtaa
     5281 tcatgaagct aaccgatgcg gataatgccg ccgatggcat ttttttcccc gcccttgagc
     5341 aaaatatgat gggtgcggtg ttaattaacg aaaatgatga agtgatgttt ttcaaccccg
     5401 ccgcagagaa gctctggggg tacaaacgtg aagaagtcat tggcaataac attgatatgc
     5461 tgattccgcg ggatttgcgt cctgcgcatc ctgaatacat tcgtcataac cgtgaaggcg
     5521 gtaaagcgcg tgtcgagggg atgagtcggg agctgcagct ggagaaaaaa gacggcagta
     5581 aaatctggac ccgttttgcg ctatcgaaag tgagcgccga ggggaaagtt tattacctgg
     5641 cgctggtacg ggatgccagc gtagaaatgg cgcaaaaaga acagactcga caattgatta
     5701 ttgccgttga ccatctcgac cgaccggtga ttgtcctcga tccggaacgc catattgtgc
     5761 agtgcaatcg cgcatttacc gaaatgtttg gttactgcat tagcgaagcc agcggtatgc
     5821 agcccgatac actcctgaac attcctgaat tccctgccga taaccgcatt cgtttacaac
     5881 agttgctatg gaaaaccgcc cgcgatcagg acgaatttct gctgttgacg cgcaccggtg
     5941 aaaaaatctg gattaaagcc tctatcagcc cggtttatga cgtgctcgcg catctgcaga
     6001 acctggtaat gactttctcg gatatcaccg aagaacggca aatccgccag cttgaaggca
     6061 atattctcgc cgccatgtgc agcagcccgc catttcatga aatgggggaa atcatttgtc
     6121 gtaacatcga atctgttctc aacgaatcgc atgtttcgct gttcgcactg cgcaacggga
     6181 tgccgataca ctgggcgtca tcttcgcacg gtgcagaagt tcaaaatgcg caaagctggt
     6241 cagcgaccat tcgtcagcgt gatggcgcgc ctgcggggat cctgcaaatt aaaacctcgt
     6301 caggagcaga aaccagcgcc tttatcgaac gcgtggcaga tatcagtcag catatggccg
     6361 cgctggcgct ggaacaggaa aaaagccgcc agcatattga acaactcatc caatttgatc
     6421 cgatgaccgg tctgccaaat cgcaataacc tgcacaatta cctcgatgac ctggtcgaca
     6481 aagccgtctc tcccgtggtg tatctcatcg gtgttgacca tattcaggat gtgattgata
     6541 gccttggcta tgcgtgggcc gatcaggcat tgctggaagt ggtcaatcgc tttcgtgaaa
     6601 aactcaaacc ggatcagtat ctctgtcgta tcgaaggtac gcagtttgtc ctcgtgagcc
     6661 tggaaaacga cgtcagtaac attacccaga tcgccgatga gctacggaat gtggtcagca
     6721 agccgataat gattgacgat aaaccattcc cgcttacctt gagtattggc atcagctacg
     6781 acgtgggtaa aaaccgcgat tacctgctct ccactgctca caatgcaatg gattatattc
     6841 gcaagaatgg cggtaacggc tggcagttct tcagcccggc gatgaacgaa atggtaaaag
     6901 agcgtttggt tttaggcgca gcgctgaaag aagcgattag caataaccaa ctgaaacttg
     6961 tttaccagcc gcaaatcttc gcagaaacgg gtgaactgta cggcatcgaa gcccttgcgc
     7021 gctggcacga tccccagcat ggtcatgtgc ccccttcacg gtttattcct ctcgcagaag
     7081 agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc cgtcagttag
     7141 cagaatggcg tagccagaat attcatatcc cggcgctttc cgttaacttg tcggcactgc
     7201 actttcgcag taatcaactg cctaatcagg tgtctgatgc aatgcacgcc tggggtattg
     7261 acggccacca gctgacggta gaaatcacgg aaagcatgat gatggaacac gataccgaaa
     7321 tctttaagcg cattcagatc ctgcgtgata tgggcgtggg cttatcggta gatgattttg
     7381 gtacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa atcaaaattg
     7441 acaaaagttt tgtcgatcgt tgtctgacgg aaaaacgcat cctggcctta cttgaagcca
     7501 ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggggtc gaaaccaaag
     7561 agcaatttga gatgctacgt aagatccact gtcgcgttat tcagggatat ttcttttccc
     7621 gcccccttcc cgccgaagaa attccaggct ggatgagcag cgtgttaccg ctgaaaatct
     7681 gacaaattcc tctcgcccgc actcgcgggt tttcctttaa ggtgacactg tcacttaaat
     7741 actgtgattt cagctacgat tccgggagat tccttcctta acttcccctc ttgcaccaaa
     7801 actgcactac gctgcacaaa tgttgatcaa aagttaaaaa atataacttt aagccagcat
     7861 tctggcgctt atcccggcat ggcatgagat ctgcataagc ggaaagcgca gcaatttttg
     7921 tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag ccgtaatctt
     7981 ccctgatctg gagatcgaat tgaaatacgc ctgcgctgat aacatcacag gtaaagctat
     8041 ttatcagcaa gcgcgttgtc tgctacacaa ggatgcgatt accgcgctgg cgaaaagtat
     8101 cagtatcgcc cagctgtcag gcttacaact ggtgatttac gatgcgtatc gcccacaaca
     8161 agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg atgtgacggt
     8221 cggttctaat cacagccgtg gcacggcgat cgacctgacg cttcgtgatg aacacgggaa
     8281 catcctcgat atgggcgcag ggttcgatga aatgcacgag cgctcccatg cctatcaccc
     8341 ttccgtcccg cccgccgctc agcgcaatcg gctgttgctg aatgcgataa tgactggtgg
     8401 cggttttgtc ggtatctcca gcgaatggtg gcacttcgaa ttaccccagg cagcgagtta
     8461 ccctctgctc gctgatcaat tctcttgttt tatatcccct ggcacacagc acgtcagtta
     8521 atcaggaaac tgtcatgaag agatcgatat cgtttcgtcc cacattgctc gcgctcgtcc
     8581 ttgccacaaa tttcccggtt gcgcacgccg ccgtaccaaa agatatgctg gtgattggta
     8641 aggccgccga tccacaaacc ctcgacccgg cggtaacaat agataataac gactggacag
     8701 tgacctaccc gtcttatcag cggctggttc agcacaaaac ggacggtgat aaaggctcaa
     8761 ccgacgttga aggcgatctg gcaagtagct ggaaagcgtc tgacgatcaa aaagagtgga
     8821 cgttcaccct gaaagataat gctaaatttg ccgatggcac acctgtcact gccgaagcag
     8881 taaaactttc ttttgagcgg ctactaaaaa tcggccaggg gccagcagaa gcatttccca
     8941 aagatttaaa gattgatgct cccgacgaac atacggtgaa gtttaccctt agccaaccat
     9001 tcgcaccgtt cctctacacg ctggcgaatg acggtgcatc cattatcaat ccggcggtct
     9061 taaaggaaca tgcagcggat gatgctcgcg gcttcctcgc gcaaaatacc gccggttccg
     9121 gaccatttat gctgaaaagc tggcaaaaag gtcagcaatt agttctggtg ccaaatccgc
     9181 attacccagg caataaaccg aacttcaaac gggtatcggt aaaaattatc ggtgaaagtg
     9241 cctcccgtcg cctgcagctc tcccgtggcg acattgacat tgccgatgcg ctgccggtgg
     9301 atcaactcaa cgccctgaag caggaaaata aagtcaatgt ggcagagtat ccgtcactgc
     9361 gcgttaccta tctgtatctg aataacagca aagcgcctct taatcaggcg gatctgcgtc
     9421 gggccatttc ctggtctacc gattatcagg gcatggttaa cggcattctg agtggtaacg
     9481 gaaaacagat gcgcggcccg attccggaag gcatgtgggg ctacgatgcg acggcaatgc
     9541 aatacaacca tgacgaaacg aaagccaaag ccgaatggga taaagtgacg agcaaaccca
     9601 ccagcctgac gtttctctac tccgataacg atccgaactg ggagcctatt gctctggcga
     9661 cacaatccag tctcaacaag ctgggcatca atgtgaagct ggaaaagctg gcgaacgcca
     9721 ccatgcgcga cagagtgggt aaaggtgatt acgacattgc gattggcaac tggagtccag
     9781 attttgccga cccgtatatg tttatgaatt actggtttga gtccgacaaa aaaggtctgc
     9841 cgggtaaccg ctcgttctat gaaaacagtg aggtcgataa gttactgcgc aatgcgctcg
     9901 cgaccaccga ccagacgcag cgtacccggg actaccagca ggcacagaaa atcgtcattg
     9961 atgacgctgc ttatgtgtac ctgttccaga aaaactacca actggcgatg aacaaagagg
    10021 tgaaaggctt tgtgttcaat cccatgctgg aacaggtctt caatatcaat accatgagta
    10081 aataacgtga ccttctggag tattttacgc caacgctgct gggggctggt gctcgtggtg
    10141 gcgggggtct gtgtgattac gtttattatc tcgcacctga tccccggcga tccggcgcgg
    10201 ttactggcag gtgaccgcgc cagcgatgct atcgtggaaa atattcgcca gcaactgggg
    10261 ctggaccagc cactgtacgt acagttttac cgctacgtca gcgacctgtt tcatggtgac
    10321 ctgggaacat ccattcgtac cgggcgtccg gtgctggaag agttgcgtat atttttcccg
    10381 gcgacgctgg agctggcttt ttgtgccctg ctgctggcac tcctgattgg cattccgctg
    10441 ggcatactct ctgcagtctg gcgaaatcgc tggctggatc atctggtgcg aataatggcc
    10501 attaccggaa tctccacacc tgcgttctgg cttgggctgg gcgtcattgt gctgttttat
    10561 ggtcatctgc aaatacttcc cggcggcgga aggcttgatg actggctgga tccaccaacg
    10621 cacgttaccg gcttttatct gctcgatgcg ttgcttgaag gcaacggtga agtcttcttc
    10681 aatgcgttgc aacgtctcat cttaccggca ttaacgctgg cgtttgttca cctggggatt
    10741 gtcgcccgcc agattcgctc agcgatgctg gaacaattga gtgaagacta cattcgtacc
    10801 gcccgggcca gcggcttgcc cggctggtat atcgttctat gttatgcgct acccaatgcg
    10861 ttgatcccat cgattaccgt attggggctg gcgctgggcg atttgttgta tggcgcagtg
    10921 ctcaccgaaa ccgtttttgc ctggcccgga atgggtgcat gggtagtaac atcaatacag
    10981 gcgctcgact tcccggcagt gatgggcttt gccgtcgtgg tttcatttgc ttatgtgctg
    11041 gtcaacctgg tggtggattt gctctatttg tggattgatc cgcgtatcgg acgtggaggt
    11101 ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa acgcgattta
    11161 acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc ggcgcagtca
    11221 tcattgtatt aatgctattg atgatgattt tttcaccgtg gcttgcgacg catgatccca
    11281 acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg tttggcaccg
    11341 atgaagtggg acgcgatctg ttcagccgcg tactggtcgg cagtcagcaa tcaattctcg
    11401 ccggattagt ggtggtcgcc attgcgggta tgattggttc gctactcgga tgtctatccg
    11461 gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt atgctgtcga
    11521 ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt ttatttaacg
    11581 ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcctggcg cgggggcaag
    11641 cattagttgt acgccagtat acttatgttc aggcggcgaa aacctttggc gcgtctcgtt
    11701 ggcatctgat caactggcat attttacgta actccctgcc gccgctgatc gtgcaggcat
    11761 cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt ggcctgggtg
    11821 cacaacaacc gagtgctgaa tggggcgcga tggtagcgaa tggtcgcaac tatgtactcg
    11881 atcagtggtg gtattgcgca tttccggggg cagcgatttt gcttaccgcc gtcgggttta
    11941 atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga aagcagtcat
    12001 gacccaaccc gttctggaca ttcaacaact gcatttgagt ttacccggtt ttaacggtga
    12061 tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc agtgaaattg tcggtctggt
    12121 gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc tgctaccgac
    12181 gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagaagatg ttcttaacgc
    12241 tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc
    12301 gatgaccgcc ctcaatccga cacgtcgaat aggtcttcag atgatggacg taatccgcca
    12361 tcatcaacca ataagtcgtc aggaagccag agctaaagcg attgccctgc tggaagagat
    12421 gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt caggtggtat
    12481 gcgccagcgg gtaatgattg cgctggcatt ctcctgcgag ccgcaattga ttattgccga
    12541 cgaaccgact acggcgctgg acgtcacggt acagttgcag gtactgcgtt tgcttaaaca
    12601 taaagcccgc gccagtggaa ctgcggtact gttcatcagc catgatatgg ccgtggtgtc
    12661 gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa gcggcgtgac
    12721 ggcagacgtt atccatcatc cccggcatcc gtataccatt ggtttgctac aatgcgcacc
    12781 agaacatgga gtaccacgcc agccattacc agccattcca gggacggtac caaacctcac
    12841 ccatttgcct gacggctgcg cttttcgcga tcgttgctat gcggcaggtg cacagtgtga
    12901 aaacgtcccg gcgctgacag cgtgtggtga caacaaccag cgctgcgcct gttggtatcc
    12961 gcagcaggag gtcataagtg tctaacacgt tattaacgtt atgcgacgtc catatcaatt
    13021 tcccggcccg taaaaactgg cttggtaaaa ctacggaaca tgttcatgcc attaatggta
    13081 ttgatttaca gatccgccgt ggtgaaacct tagggatcgt cggcgagtca ggctgcggca
    13141 aaagcaccct cgcacagctt ttaatgggta tgctgcaacc gagccacggg cagtacatcc
    13201 gttcaggatc acaacgcatt atgcagatgg tgtttcagga cccgctctct tcgctcaatc
    13261 cgcgcttacc ggtgtggcgc atcatcacag aaccgctctg gatagctaag cgtagtagtg
    13321 aacaacagcg gcgagcgtta gcagaggagt tggctgtgca ggtgggtatt cgtccggagt
    13381 atctcgaccg cctgcctcat gcgttctccg gcgggcagcg gcaacgcatc gccattgcca
    13441 gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctct gcactggata
    13501 tctccgtgca ggcgcagatc ctcaatttac tggtaacgct acaggtaaat cgcgggctga
    13561 cctatgtgct gatctcacac aatgtctcgg tgattcgtca tatgagcgat cgggtagcgg
    13621 tgatgtatct cgggcagatt gtagaactgg gcgacgcgca gcaggtgttg acggcacctg
    13681 cacatccata cacccgatta ttgctggatt ccctccccgc cattgataaa ccgctggagg
    13741 aagaatgggc attacgtaaa acggatctgc cgggaaaccg cacgttgccg caaggctgtt
    13801 ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa tcattaacaa
    13861 taagggagga cggacgtgag atccggtgct ggcgggcgct gtagagatgc taacaacgca
    13921 tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca actggtaatc
    13981 aagcgtaatt tccgctttca gtacctgaga aaccgggcat cctgcttttg ctttctggat
    14041 gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct tcagtgcgat
    14101 tttcgtaatc gcaaaaccgg catccacttt atccagcgac acatcggcgg tggtatcaat
    14161 cgatgttggc gtaaatcccg cttcccccag cattaatgaa agcgccattg agaaacatgc
    14221 ggcatgcgct gcgccaatca gttcttcagg gttagttcct ttttcgcctt caaaacgcgt
    14281 gttaaatcca tacggctgtt ggttcagcac accactctcg gtggatactg ttcccttccc
    14341 gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca ttgttgctct
    14401 cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg ataagcagga
    14461 tattccgaat aaaaatccgg cagaaatatt acttctgcac ttaaattaaa tttccacaat
    14521 agataaccgg gaatatataa cccaagagat attgcattaa tattaatcaa ctcaccacaa
    14581 taataatacc tatcaatatc atgaggttaa aatataaaaa tcatcaacca ggactaatct
    14641 taacaacgaa gcggcaaata tttgccatgc tgaatgtgct ttataaatct gcgatccgta
    14701 gcagacacca taaatacaca gacacggaga atcactatgt ttacttatta tcaggcagaa
    14761 aattcaacag cagaacccgc tctggttaat gccattgagc aaggccttcg ggcagagcac
    14821 ggtgttgtca ctgaagatga cattctcatg gagctgacca aatgggtgga agcttctgat
    14881 aacgacatcc tcagtgatat ctaccagcaa acgatcaatt atgtggtcag tggccagcac
    14941 cctacgcttt aaggtgctat gcttgatcgg caacctaatt taggggttta gcacgtgttt
    15001 cttcgctacg gcgatgttgt ccttaaaact agcaacagga ttgaggagtt aaaatgaaat
    15061 cgaaccgtca ggcacgtcat attcttggac tggaccataa aatttctaac cagcgcaaaa
    15121 tagttaccga aggtgacaaa tccagcgtgg taaataatcc aaccggcaga aaacgccccg
    15181 ctgaaaagta attcataacc atcagtcctc aatgacgatt aaacaccatt gcctgcgcaa
    15241 tggtgttttt gtttttatct gctctatact tggggccgac gccctggcgg taaagcaaag
    15301 acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg gaaccaaaaa
    15361 caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg gaatttccgt
    15421 tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac ctgctggggt
    15481 tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg atccagtatc
    15541 agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag gacaccaacg
    15601 aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct gttatttata
    15661 ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt tcacgcggcg
    15721 tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac gtaccaaacc
    15781 ataatatcaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt ggtgaccagg
    15841 gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc tgtggcggca
    15901 tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac caacagttgc
    15961 ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgac gaatactatg
    16021 aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg ctgttgcagt
    16081 ttgaagactt cgcacaaaaa aatgcgatgc cgttacttaa ccgctatcgc aatgaaattt
    16141 gttcctttaa cgatgacatt cagggcactg cggcggtaac agtcggcacc ctgatcgcag
    16201 caagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt ggcgcaggtt
    16261 cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa ggattaagcg
    16321 aggaagcggc gcggcagaaa gtctttatgg tcgatcgctt tggcttgctg accgacaaga
    16381 tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac ctcagtgact
    16441 gggataccga cagcgatgtg ctgtcactgc tggatgtggt gcgcaatgta aaaccagata
    16501 ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc cgtgagatgc
    16561 ataaacactg tccgcgtccg atcgtgatgc cgctgtccaa cccgacgtca cgcgtggaag
    16621 ccacaccgca ggacattatc gcctggactg aaggtaacgc gctggtcgcc actggcagtc
    16681 cgtttaatcc agtggtatgg aaagataaaa tctaccctat cgcccagtgt aacaacgcct
    16741 ttattttccc aggcatcggg ctgggtgtta ttgcttccgg cgcgtcacgt atcaccgatg
    16801 agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc ctgaacggcg
    16861 aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca attgcgtttg
    16921 cggttggcaa aatggcgcag cagcaaggcg tggcggtgaa aacctctgcc gaagccctgc
    16981 aacaggccat tgacgataat ttctggcaag ccgaataccg cgactaccgc cgtacctcca
    17041 tctaagcctg cgcccggtag tgaaggctac cgggctattt ccctctccct ttttcagatc
    17101 tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat gcagaacatc
    17161 atccgaaaag gaggaactat gaaggctgca gttgttacga aggatcatca tgttgacgtt
    17221 acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat ggagtgttgt
    17281 ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa aaccggcgta
    17341 attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt cacctcatta
    17401 aaaccaggcg atcgtgccag cgtggcgtgg ttctacgaag gatgcggtca ttgcgaatac
    17461 tgtaatagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata cagcgttgat
    17521 ggcgggatgg cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt
    17581 ctggactcgg cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt
    17641 aaactgtcaa aaattcgtcc agggcagtgg attgctatct acggtcttgg cggtctgggt
    17701 aacctcgctc tgcaatacgc gaagaatgtc tttaacgcga aagtgatcgc cattgatgtc
    17761 aatgatgagc agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc
    17821 accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc tgcggtggta
    17881 acagcagttg ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt
    17941 gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg ccttgtgctg
    18001 gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc
    18061 cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc
    18121 aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc
    18181 cgccgttaag aggcctttgc tgcgagtgcc agtcctggtg tcgcagcatc gcactctctc
    18241 cgacattaaa tggctaaatg gattattgtt ccttcctacg caacaataat gatagtgttc
    18301 ctgatgaagg aacactactg agagtaaaga cactgatgaa aatggccaat catccccgcc
    18361 cgggggacat tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca
    18421 gagcaatgga aattgctccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga
    18481 caccagaaat ggcgattaaa cttcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
    18541 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgactacg ccgtctggtt
    18601 acgcaataaa aaaatggcgc ttcttaaagc gccattttca gcttaaagtt caaaagagat
    18661 tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc
    18721 ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac
    18781 cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc
    18841 gcagccgcgt ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag
    18901 tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac
    18961 cagaacatca tcttttgccc ggcgttgtac ttaccgacat ccgccacttt atgctcattg
    19021 cctttcaata cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg
    19081 tgatgcacaa aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaaaaac
    19141 gggtgcaaaa tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc
    19201 gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag
    19261 tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc
    19321 ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc
    19381 ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct
    19441 taccgaggtg tcgatcttcg gatctttcgg cagaccgtga tacagctccg gctgatcggc
    19501 gtgatgcagc acgtacataa cgtgcgtacc accgacccct tccgggttgt agacgccagc
    19561 atgttcgtaa tcacgcgctt tcagtttcgc cacgcgctgt tccgccagct ccagcatctc
    19621 cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg
    19681 gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt
    19741 gaggcgcgga atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc
    19801 cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc
    19861 cttcaggcag ccgggatctt cacagtgcat acagccgtct ttacggatca gccactccag
    19921 cttgccgttc tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc
    19981 ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc
    20041 cgaacacgcc acctgacagg ctttacaacc gatacaggtg gaaacgtcga taagtttcgc
    20101 gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct
    20161 tttgataatg tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt
    20221 gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag
    20281 agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc
    20341 caccgtttca acctgctgac catttacatt cagtggtttc agacgacgcg ttaccacagc
    20401 caccgcgcgg ataaagccac gcttgcttga gacagtgaca cgatcgccat tattaatgcc
    20461 tttcgccgcc gccagcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt
    20521 gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac
    20581 atacgggaac tgctcttttt tacccatccg cagtgcgtct tgttcataca gacgaacaac
    20641 cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc
    20701 gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc
    20761 ttccggctgc atgataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtcagg
    20821 aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca
    20881 cggtttaccg ttgatatccg ccgaagcacg gttgtacagc acgcgacggt tgagcggcca
    20941 cgcccaggcc catcccagcg tattccccag accggacggg tcggagttat cgcggttagc
    21001 catctggttg ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt
    21061 accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac
    21121 gccattagca tcatagagat cttccagcgc atagccgttg ttctctttag ctacttcgtc
    21181 agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc
    21241 tttaccacct tcggcctggt acagctcgcg caggtgatgg tagatacccg ccagaatttc
    21301 accgtcgtta cgcgcttcgc ccggcgcatc ctgacctttc cagtgccact gcagccagcg
    21361 accggagtta gcaatagaac catcttcttc agcaaagcag gtcgaaggca gacggaatac
    21421 ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt
    21481 tgaggtttca gtcaccagcg gatcgataac caccatgtac ttcagcttgc ttaggcagct
    21541 caccactttg tttttgtccg ggaaggacgc aaccgggtta aagccctggc agaaataacc
    21601 ggtgactttg ccttcatcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca
    21661 cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga
    21721 tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct gaccagccag
    21781 cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg
    21841 cagcgtcaga taacctggca ggctggtaga gagcaggcct aagtcagtca gaccctgaat
    21901 gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag
    21961 caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc
    22021 cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca
    22081 cactttcagg aagtcggctt ttggcgtacc acagatgttt tcaacaacgt ccggcgtgta
    22141 gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc
    22201 gcgtttcgca tagccgtttt catcgaactg atagttccag gacgatttat cgtattgccg
    22261 tttttcagcg tcgtagccgc tgaacagacc gtcttcgaaa gcaaaatcat cacgcaccag
    22321 caggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag
    22381 gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat
    22441 atccgccaca gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg ttgcatcgtt
    22501 gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc
    22561 gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg tcatcgcacc
    22621 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt
    22681 gtctaccgcc agcatcccga gggagcgggc aaatttctgt gtcagcatcc cggtttcgtt
    22741 gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttactg ttacgccctg
    22801 ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagctttg caatacggga
    22861 gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatattccgg
    22921 gtagcgcaga cggttttcgc tgttgacgta atccagcaaa ccggcccctt ttgggcacag
    22981 cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc
    23041 gtttttcgcg ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt
    23101 gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc
    23161 ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat
    23221 tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg
    23281 cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg
    23341 tttgccacaa aataatccgc ctgcgcaaat tgacctacct caatagcggt agaaaaacgc
    23401 accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc
    23461 aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg
    23521 atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggcccg
    23581 gtcggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga
    23641 tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc
    23701 agctatgaaa tctgtctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt
    23761 gaagtgggta tggtgaacta tctatggccc agcctgacaa ttctctttgc cattctgttt
    23821 aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc
    23881 tgctgggtgt taggcggtga taatgggtta cattatgatg aaatcatcaa taatattacc
    23941 accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc
    24001 acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg
    24061 ggagcaagtc tgtgggttta ctattttctc acgccacaac cagaaatggt atttagcacg
    24121 cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta tgctgcatgg
    24181 aatgtcggta tattgcatgg taacgtcacc ataatggcgg taggttcgta ttttacgccc
    24241 gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg
    24301 caaggcgcgc taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt
    24361 taaaataatt gccgggtcta cggcccggca gttaatcaga taaattccga caatattaaa
    24421 aaat
//
LOCUS       DAZFFI010000068        23479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFFI010000068 DAZFFI010000000
VERSION     DAZFFI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.068"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(372..1169)
                     /locus_tag="ACHJ12_003132"
     CDS             complement(372..1169)
                     /locus_tag="ACHJ12_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4990884.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1179..1730)
                     /gene="ybcL"
                     /locus_tag="ACHJ12_003133"
     CDS             complement(1179..1730)
                     /gene="ybcL"
                     /locus_tag="ACHJ12_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO4990885.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1899..2231)
                     /gene="emrE"
                     /locus_tag="ACHJ12_003134"
     CDS             complement(1899..2231)
                     /gene="emrE"
                     /locus_tag="ACHJ12_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO4990886.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2412..2531
                     /locus_tag="ACHJ12_003135"
                     /pseudo
     CDS             2412..2531
                     /locus_tag="ACHJ12_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2565..2879)
                     /gene="fliE"
                     /locus_tag="ACHJ12_003136"
     CDS             complement(2565..2879)
                     /gene="fliE"
                     /locus_tag="ACHJ12_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4990887.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3094..4752
                     /gene="fliF"
                     /locus_tag="ACHJ12_003137"
     CDS             3094..4752
                     /gene="fliF"
                     /locus_tag="ACHJ12_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4990888.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPAEKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4745..5740
                     /gene="fliG"
                     /locus_tag="ACHJ12_003138"
     CDS             4745..5740
                     /gene="fliG"
                     /locus_tag="ACHJ12_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4990889.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5733..6419
                     /gene="fliH"
                     /locus_tag="ACHJ12_003139"
     CDS             5733..6419
                     /gene="fliH"
                     /locus_tag="ACHJ12_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4990890.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6419..7792
                     /gene="fliI"
                     /locus_tag="ACHJ12_003140"
     CDS             6419..7792
                     /gene="fliI"
                     /locus_tag="ACHJ12_003140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4990891.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPTVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7811..8254
                     /gene="fliJ"
                     /locus_tag="ACHJ12_003141"
     CDS             7811..8254
                     /gene="fliJ"
                     /locus_tag="ACHJ12_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4990892.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8251..9378
                     /gene="fliK"
                     /locus_tag="ACHJ12_003142"
     CDS             8251..9378
                     /gene="fliK"
                     /locus_tag="ACHJ12_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4990893.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            9483..9947
                     /gene="fliL"
                     /locus_tag="ACHJ12_003143"
     CDS             9483..9947
                     /gene="fliL"
                     /locus_tag="ACHJ12_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4990894.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9952..10956
                     /gene="fliM"
                     /locus_tag="ACHJ12_003144"
     CDS             9952..10956
                     /gene="fliM"
                     /locus_tag="ACHJ12_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4990895.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10953..11366
                     /gene="fliN"
                     /locus_tag="ACHJ12_003145"
     CDS             10953..11366
                     /gene="fliN"
                     /locus_tag="ACHJ12_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4990896.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11369..11734
                     /gene="fliO"
                     /locus_tag="ACHJ12_003146"
     CDS             11369..11734
                     /gene="fliO"
                     /locus_tag="ACHJ12_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4990897.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            11734..12471
                     /gene="fliP"
                     /locus_tag="ACHJ12_003147"
     CDS             11734..12471
                     /gene="fliP"
                     /locus_tag="ACHJ12_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4990898.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12481..12750
                     /gene="fliQ"
                     /locus_tag="ACHJ12_003148"
     CDS             12481..12750
                     /gene="fliQ"
                     /locus_tag="ACHJ12_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4990899.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVSTLFTNLPYIIG"
     gene            12758..13543
                     /gene="fliR"
                     /locus_tag="ACHJ12_003149"
     CDS             12758..13543
                     /gene="fliR"
                     /locus_tag="ACHJ12_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4990900.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLTLLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13833..14456
                     /gene="rcsA"
                     /locus_tag="ACHJ12_003150"
     CDS             13833..14456
                     /gene="rcsA"
                     /locus_tag="ACHJ12_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4990901.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14500..14688)
                     /gene="dsrB"
                     /locus_tag="ACHJ12_003151"
     CDS             complement(14500..14688)
                     /gene="dsrB"
                     /locus_tag="ACHJ12_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4990902.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14851..15078
                     /gene="yodD"
                     /locus_tag="ACHJ12_003152"
     CDS             14851..15078
                     /gene="yodD"
                     /locus_tag="ACHJ12_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4990903.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15376..16194
                     /gene="yedP"
                     /locus_tag="ACHJ12_003153"
     CDS             15376..16194
                     /gene="yedP"
                     /locus_tag="ACHJ12_003153"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4990904.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQQLSGKRPTTLGLGDGPN
                     DAPLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16191..17885)
                     /gene="dgcQ"
                     /locus_tag="ACHJ12_003154"
     CDS             complement(16191..17885)
                     /gene="dgcQ"
                     /locus_tag="ACHJ12_003154"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO4990905.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18056..18238)
                     /gene="yodC"
                     /locus_tag="ACHJ12_003155"
     CDS             complement(18056..18238)
                     /gene="yodC"
                     /locus_tag="ACHJ12_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4990906.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(18317..19234)
                     /gene="yedI"
                     /locus_tag="ACHJ12_003156"
     CDS             complement(18317..19234)
                     /gene="yedI"
                     /locus_tag="ACHJ12_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4990907.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19407..20327
                     /gene="yedA"
                     /locus_tag="ACHJ12_003157"
     CDS             19407..20327
                     /gene="yedA"
                     /locus_tag="ACHJ12_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4990908.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20316..20786)
                     /gene="vsr"
                     /locus_tag="ACHJ12_003158"
     CDS             complement(20316..20786)
                     /gene="vsr"
                     /locus_tag="ACHJ12_003158"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4990909.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20767..22185)
                     /gene="dcm"
                     /locus_tag="ACHJ12_003159"
     CDS             complement(20767..22185)
                     /gene="dcm"
                     /locus_tag="ACHJ12_003159"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4990910.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22252..22947)
                     /gene="yedJ"
                     /locus_tag="ACHJ12_003160"
     CDS             complement(22252..22947)
                     /gene="yedJ"
                     /locus_tag="ACHJ12_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO4990911.1"
                     /translation="MDLQHWQAEFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPHTMQTIRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22987..23268)
                     /gene="drpB"
                     /locus_tag="ACHJ12_003161"
     CDS             complement(22987..23268)
                     /gene="drpB"
                     /locus_tag="ACHJ12_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO4990912.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5797 a         6097 c         5897 g         5688 t
ORIGIN      
        1 aacagtggca atttctgctg tagctgcatc ttaacgtata aaccagattt aataacaacg
       61 aactagattt aataacaacg atatccgtac ctatccggat atctaatgtc ataagcatcc
      121 ctttaattat cccctcaacc tcttgtaact ttcccactct tgaactccac atagtccaaa
      181 cgccggatgc aaaaaaatca cacgtgcatt tctgaaattg tgatggagga agatagtatc
      241 aattatatgt gcttataaac gtccccgatt agcgcagaag tcaccggatt gttcaggctc
      301 caataggttg agtatgctgt atttattttc caaaataaaa atcgatgctg ttgctccttc
      361 ataaaatact tttactctct ggacgctttt cttagctctc ttggtgttat cttaaaacga
      421 ttatggaacc tttcagtaaa acgagaagga cacttgtaac cattttccct ggcaatctcg
      481 ctgatgggtt ttactgtcgt ttgtatagca gacaacgcat tatttaacct cacgtcgtcc
      541 aatatacttt gaaaacttac tccctcgctt gccagacggc gatgcaatgt ggaaacagaa
      601 atgtagagat atctcgctac cttgtttgct gtccattttg ttccaggttc ggatagcatt
      661 aggttataac aacgacttat taatgattgt ttactatctg ataaaagtaa acaattaaca
      721 tgatgtgctc ctaatgaaag tagaacgccc attgctaagt gttcctgaat ttgcgttgag
      781 aaacctcggg aaatagatgt ttttagttgc tcccaacaat atattaactc gggattctga
      841 ggtaagcaga aacctgtcgt gttacgaatt tgatcggtta tcgcataaag attttggaac
      901 ttttcaatta gatcaatggg ataataaagc atttctgcaa gataaagacc tgcttctggg
      961 taattcgcaa tataaaattc atatccacag ggaaataata ttatctgatt attatcaaca
     1021 gttagactat gcgtctccca attgataact ttttttccct gacggatacg acataaagct
     1081 ggcataagag gcttaaccct gtgaatctca tgatgtttat gcatccgtat ttcttcaatc
     1141 tttaggttag tcctatcttt tgccagcata ctcttatcct actttacctc ataaacgggt
     1201 attatctcag cggttgcaat tttattggca ttaagcatat aaccaactaa cgctccgctg
     1261 gaattagaat ccacaggaat cttatcagtt tttagagccc atattttaaa ctggtaatga
     1321 tggggtttat ctcctttggg aggacatgct ccaccaaatc ccgcatagcc aaaatcattt
     1381 cggccttgga cagcaccagt cggaagtttt gtaccatcac gtcttcctgc atcagcgggc
     1441 aaatatgtta ccgttgctgg aatattagca acagtccagt gccaccatcc gctgcctgta
     1501 ggcgcatctg gatcatacac agttacggca aagcttttgg tgccttctgg agcaccagac
     1561 caggttaatg agggcgatgt attacccccc tcacacccaa atccagaaaa tacatgagac
     1621 gttgtaagtt gctctcctgt ttttatttca ttactagtga cctgaaatgc tgcggcctgc
     1681 gcagaaaatg ttatgaatgc caatacactt gaaacgataa gttttttcat aaaaacctct
     1741 ttgttgtgac ctatcgttat tttatttgat gtgcattgat ctcattatgc aaaaaggcgc
     1801 aatgatcgcg caaatgcaat cataattata aagttgcccc gaacattagc cataatatgc
     1861 acaaatcaga ttcctgttat ctcaaggatg attatttttt aatgcggtgc gcttcgtgac
     1921 aacaagttaa tgactaacac accggcacaa atcaacatca tgcctatgat ggctggcaag
     1981 tccagtcgtt ggccaaaaaa tccccaggaa agcaaactaa tcagaacgat accgactcct
     2041 gaccagatag cataagcaat ccctgtaggt atataagcca gtgtctgagc taataaccaa
     2101 aatgatgcac aatagcaaat aattgtgcca acagagggcc ataaccgtgt aaacccctct
     2161 gaaaacttca ttaaggttgt accaatgacc tctgcaagta ttgcgccacc aagataaata
     2221 taagggttca tagcatattc tttcctgttc atactggaga gaattgtact acagtttgaa
     2281 cacaactcac cgttttcatc ctggtgtatg tgtatccagg gggctctggc gtggcaagag
     2341 ataaaggcag gaacaataat gatcagaagg aaccccaggc taacaaaaga gttcactctt
     2401 cctatgagca aagcgaagac aaaacatgtt atcgcgttta cggatcgtgg atggcagaaa
     2461 actttcagga tcaggtatcc attttaaacc agaaattgag tgagtttgcc ccatccatgc
     2521 cccaagcggt gagattggaa gtaattaaca acaggttaca aaacctacac ctgcatggac
     2581 atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat ttgcatagaa
     2641 actgaggctt tttgcatatc ggtcatcaca tcgtttaacg ccacgccggg ttcaccgagg
     2701 gtgaattttt ctgcctgcgt gcgggcagct gtttgtgtat cgctaatgcg atcgagcgcg
     2761 gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgaatcctg cgcacgcgca
     2821 ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat cgctgacatt
     2881 ttcgtctcct ggataatttc tggtagcaaa gcctaccagt aagtcaataa gacaaaggcg
     2941 ctaaatagca acaaaaaaac gggtttattg gcggatagaa aaaaacgaaa gcacaaataa
     3001 ttgcagcgtc aatttttcga gtttgctgac ccgggagtga gtcttgttcc actttgccaa
     3061 taacgccgtc cataatcagc cacgaggtgc gcgatgaatg cgactgcagc ccagacaaaa
     3121 tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttgccggt
     3181 tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc
     3241 acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa
     3301 atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaagttcc ggcagagaaa
     3361 gttcacgaac tgcgtctgcg cctggcacaa caaggtttgc ccaaaggcgg cgcggtcggt
     3421 ttcgaactac tcgatcagga aaagtttggt atcagccagt tcagcgaaca ggtgaattat
     3481 cagcgggcgc tggaaggcga gctttctcgt accatcgcaa ctatcggccc ggtaaaaggg
     3541 gcgcgcgtac atctggcaat gccgaaaccg tctttattcg tccgtgaaca aaaatcccct
     3601 tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac tcgatgaagg gcaaattagc
     3661 gccattgtgc atctggtttc cagcgccgtt gctggtctgc cgccgggaaa cgtcacgctg
     3721 gtggatcagg gcggacatct gttaacccag tccaatacca gcgggcgcga tcttaatgat
     3781 gctcagttga aatatgccag cgatgtcgaa ggccgtattc agcggcgtat tgaagcaatc
     3841 ctgtcgccta ttgttggtaa cggtaatatt cacgcccagg tcacggcgca gctggacttc
     3901 gccagtaaag aacaaacgga agaacagtat cgccctaacg gtgatgaatc tcatgcggcg
     3961 cttcgttcac gccagcttaa tgagagcgag caaagcggtt ccggttatcc gggcggcgta
     4021 ccgggggcgt tgtcgaatca accggcacct gcgaataacg cgccaatcag cacgcctccg
     4081 gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca gcaatagcgg gccgcgtagc
     4141 acacagcgga atgaaaccag taactacgaa gtcgatcgca ccattcgtca taccaaaatg
     4201 aacgtgggcg atgtgcaacg tctgtcagtc gcggtagtgg tgaattacaa aaccttgcca
     4261 gacggtaaac cattgcctct cagcaacgaa cagatgaagc aaattgaaga tctgacccgc
     4321 gaggcgatgg gcttttctga aaaacgcggt gactcgctca atgtcgttaa ctcgccgttc
     4381 aatagcagtg acgaaagcgg cggagaactg ccgttctggc aacagcaagc gtttatcgat
     4441 cagttgctgg ctgccggtcg ctggttgctg gtactgctgg tggcgtggct gctgtggcgg
     4501 aaagcggtac gcccgcagtt aacacgtcgc gccgaggcga tgaaagctgt acagcaacag
     4561 gcgcaggccc gtgaggaagt ggaagatgcg gtggaagtcc gcctgagcaa agacgaacaa
     4621 ctccaacaac gacgcgctaa ccaacgtctg ggggcagaag tcatgagcca gcgtatccgt
     4681 gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg gataaataac
     4741 gatcatgagt aacctgacag gcaccgataa aagcgtcatc ctgctgatga ccattggcga
     4801 agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc gaagtgcaaa ccctgagcgc
     4861 tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc tggcggagtt
     4921 tgagcaagaa gctgaacagt ttgccgcact gaatatcaac gccaacgatt atctgcgttc
     4981 ggtattggtc aaagctctgg gtgaggaacg tgccgccagc ctgctggaag atattctcga
     5041 aactcgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc agagtgccgc
     5101 cgatctgatt cgcgatgagc atccgcaaat tatcgccacc attctggtgc atctgaagcg
     5161 cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg acgtgatgtt
     5221 gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gcggaactga ccgaagtact
     5281 gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg tgagaacggc
     5341 agccgaaatt atcaacctga tgaaaactca gcaggaagaa gccgttatta ccgccgtgcg
     5401 cgaattcgac ggcgagctgg cgcaaaaaat tatcgacgag atgttcctgt tcgaaaatct
     5461 ggtggatgtc gacgatcgca gcattcagcg tctgttgcag gaagtggatt ccgaatcgct
     5521 gttgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc gcaatatgtc
     5581 gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac cgtggtccgg tgcgtctgtc
     5641 gcaggtggaa aacgaacaga aagcgattct gctgattgtg cgccgccttg ccgaaactgg
     5701 cgagatggta attggcagcg gcgaggatac ctatgtctga taatctgccg tggaaaacct
     5761 ggacgccgga cgatctcgcg ccaccacagg cagagtttgt gcccatggtc gagccggaag
     5821 aaaccatcat tgaagaggcc gaacccagcc ttgagcagca actggcgcaa ctgcaaatgc
     5881 aggcccatga gcaaggttat caggcgggga ttgccgaagg tcgccagcaa ggtcatgagc
     5941 agggctatca ggaaggactg gcccgggggc tggagcaagg tctggcagag gcgaagtctc
     6001 aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa actacccttg
     6061 atgcgcttga tagtgtgatt gcttcgcgcc tgatgcagat ggcgctggag gcggcacgtc
     6121 aggtgatcgg tcagacgcca acagtggata actcggcact gatcaaacag atccaacagt
     6181 tgttgcagca agaaccgtta ttcagcggta aaccacagct gcgcgtgcac ccggatgatc
     6241 tgcaacgtgt ggatgatatg ctcggcgcaa ccttaagttt gcatggctgg cgcttgcggg
     6301 gcgatcccac actccatcct ggcggctgta aagtctccgc cgatgaaggc gatctcgacg
     6361 ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga gtggtgtaat
     6421 gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca aaatggcgca
     6481 gttgcctacg gtacgtcgct acgggcgatt aacccgcgct accgggctgg tgctggaagc
     6541 caccggatta caattgccgc tcggcgcaac ctgtgtcatt gaacgccaga acggcactga
     6601 aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt ttttaatgcc
     6661 gctggaggaa gtcgaaggtg tcctgcccgg cgcgcgggtt tatgccaaaa acatttcggc
     6721 tgaaggactg caaagcggca agcagttgcc gctcggtccg gcgttattag gtcgcgttct
     6781 ggacggcagc ggtaagccgc tcgatggcct gccctccccc gatacgacgg aaaccggtgc
     6841 gctgattacc ccgccattta acccgttgca acgtacaccg attgaacatg tgctggacac
     6901 cggcgtgcgc ccaatcaatg ccctgcttac cgttgggcgc gggcagcgta tggggctgtt
     6961 tgccggatct ggcgttggta aaagtgtgct gctggggatg atggcccgtt acacccgcgc
     7021 cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgt gaagtaaaag attttattga
     7081 gaatatcctc ggtgctgaag ggcgtgcacg ctcagtagtg attgccgctc cggcggatgt
     7141 ttctcctctc ctgcgaatgc agggtgccgc ctatgccacg cgcattgccg aagattttcg
     7201 cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg cgatggccca
     7261 gcgtgaaatt gcgctggcga ttggcgaacc acccgccact aaaggttatc ccccgtcggt
     7321 gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat ggcattagcg gcggcggctc
     7381 gattaccgcg ttttataccg tgctcaccga gggcgatgac cagcaggacc ccattgccga
     7441 ctccgcgcgg gcaatcctcg acggtcacat tgtgctgtct cgccgactgg cggaagccgg
     7501 gcactatccg gctatcgata ttgaagcgtc gatcagccgc gcaatgacgg cgttgatcag
     7561 tgagcaacat tacgcgcgag tgcgcacatt caaacagctg ttgtcgagtt ttcagcgtaa
     7621 ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagt gatccgatgc tcgataaagc
     7681 catcgccctg tggccgcagc tggagggcta tttacaacaa ggcatttttg aacgtgcgga
     7741 ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtgtcat aacccaggag
     7801 ataacggcag atggcagaac atggcgcgct ggcgaccctg aaagatctgg cagaaaaaga
     7861 ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc gggtgtcagc aggcggaaga
     7921 acagctcaaa atgctgatcg attatcagaa tgaatatcgc aataacctca acagcgatat
     7981 gagtgccggg atgaccagca accgctggat caactatcag cagtttatcc agacgttgga
     8041 aaaagccatt actcagcatc gccagcaact taatcagtgg acgcagaaag ttgacattgc
     8101 cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc tggcagacac tgcaggaacg
     8161 gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt
     8221 cgcccagcgc gccgccatga ggaaacctga atgattcgct tagcgccctt gattaccgcc
     8281 gacgttgaca ccaccacatt gcctggcggc aaagccagcg atgctgcaca agattttctc
     8341 gcgttgttga gcgaagcatt agcaggcgag acaactaccg acaaagcggc cccccaggtg
     8401 ctggtggcaa cagataagcc cacgacaaaa ggcgagctgc tgatcagcga tattgtttcc
     8461 gacgcgcaac aagcggattt actgatccct gtggatgaaa cactgcctgt catcaacgtc
     8521 gaacaatcca catcaacacc gttaaccacc gctcagacga tgacgttggc tgcggtggct
     8581 gacaaaaata cgacaaaaga cgaaaaagcg gatgatctga atgaagacgt caccgcaagc
     8641 ctgagcgccc tttttgcgat gttgccgggt tttgacaata cgcccaaagt gactgatgtg
     8701 ccgtcaaccg tgttaccggc agagaaacca acgctcttca caaaactgac ttctgcgcaa
     8761 ctcacaacag cacagcctga tgatgccccc ggcacgccag ctcagccatt aacaccgctg
     8821 gtagcagaag cccagagtaa agcggaagtc atcagcacac cgtcaccggt gaccgctgcc
     8881 gccagcccgc taattactcc acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt
     8941 ttgagtgcac cgctgggttc tcacgaatgg caacaatcat taagccagca tatttcgcta
     9001 ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc acccgcagga tttaggtgaa
     9061 gtgcaaatct ccctcaaagt ggatgataac caggcacaaa tccagatggt ttcaccgcat
     9121 cagcatgtac gcgccgcgct ggaagcagcg cttccggtac tgcgtacgca gctggccgaa
     9181 agtggcattc agttagggca aagcaacatc agtggcgaaa gctttagtgg tcagcagcag
     9241 gccgcttccc aacaacagca aagccaacgc acagtaaacc atgaacctct ggcgggggaa
     9301 gacgacgata cgcttccggt tcccgtctct atacaagggc gcgtaacagg caacagcggc
     9361 gttgatattt tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt ccccgctttg
     9421 ttgcgctcaa gacgcaggat aattagccga taagcagtag cgacacagga agaccgcaac
     9481 acatgactga ttacgcgata agcaagaaaa gcaagcgatc gctttggatc ccgattctgg
     9541 tattcattac cctcgcggcc tgtgccagcg caggttacag ctactggcat tcgcatcagg
     9601 ttgccgctga cgacaaagcg cagcaacgtg tcgtgccctc accggtcttc tatgcgctgg
     9661 ataccttcac ggtcaatttg ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc
     9721 gcctgaaaga tgaagctacc cgctcgcggc tgagtgagta tttgccggaa gtccgtagcc
     9781 gtttgctgtt actgttttcg cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga
     9841 aaaacctgat tgccgagatt aaaaccacac tttccacccc gcttgttgcc gggcaaccga
     9901 aacaggatgt caccgacgtg ctgtataccg cttttattct gcgataacga catgggcgat
     9961 agtattcttt ctcaagctga aattgatgcg ctgttgaatg gtgacagcga agtcaaagac
    10021 gaaccgacag ccagtgttag cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa
    10081 cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc
    10141 cattttcgta tggggctgtt caacctgctg cgtcgtagcc cggatataac cgtcggggcc
    10201 atccgcattc agccgtacca tgaatttgcc cgcaacctgc cggtgccgac caacctgaac
    10261 cttatccatc tgaaaccgct gcgcggcaca gggctggtgg tgttttcacc gagtctggtg
    10321 tttatcgccg tggataacct gtttggcggc gatggacgct tcccgaccaa agtggaaggt
    10381 cgcgagttta cccataccga acagcgcgtc atcaaccgca tgttaaagct ggcgcttgaa
    10441 ggctatagcg acgcctggaa ggcgattaat ccgctggaag ttgagtacgt gcgttcggaa
    10501 atgcaggtga aatttaccaa tatcaccacc tcgccgaacg acattgtggt taacacgccg
    10561 ttccatgtgg agattggcaa cctgaccggc gaatttaata tctgtctgcc attcagcatg
    10621 attgagccgc tacgggaatt gttggttaac ccgccgctgg aaaactcgcg taatgaagat
    10681 cagaactggc gcgataacct ggtgcgccag gtgcagcatt cacagctgga gttggtcgcc
    10741 aactttgccg atatctcgct acgcctgtcg cagattttaa aactgaaacc cggcgacgtc
    10801 ctgccgatag aaaaacccga tcgcatcatc gctcatgttg acggcgttcc ggtactgacc
    10861 agtcagtacg gcaccctcaa cggtcagtat gcgttacgga tagaacattt gattaacccg
    10921 attttaaatt ctctgaacga ggaacagccc aaatgagtga catgaataat ccggccgatg
    10981 acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt gagcgaacaa aaatcaacca
    11041 gcagcaaaag cgctgccgac gcggtgttcc agcaatttgg cggtggtgat gtcagcggaa
    11101 cgttgcagga tatcgacctg ataatggata ttccggtcaa gctgaccgtc gagctgggcc
    11161 gtacgcggat gaccatcaaa gagctgttgc gtctgacgca agggtccgtc gtggcgctgg
    11221 acggtctggc gggcgaacca ctggatattc tgatcaacgg ttatttaatt gcccagggcg
    11281 aagtggtggt cgttgccgat aaatatggcg tgcggatcac cgatatcatt actccgtctg
    11341 agcgaatgcg ccgcctgagc cgttagtgat gaataaccac gctactgtgc aatcttccgc
    11401 gccggtttct gctgcgccac tgctgcaggt gagcggcgca ctcatcgcca ttattgccct
    11461 gatcctcgct gctgcctggc tggtaaaacg gttgggattt gccccgaaac gcactggcgt
    11521 taacggtctg aaaattagcg ccagtgcttc gctgggcgcg cgtgaacggg ttgtggtggt
    11581 cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca gggcaaatca atctgctgca
    11641 taaacttccc ccttcagcac caacggaaga gataccgcag cccgattttc agtcggtcat
    11701 gaaaaacttg cttaagcgta gcgggagatc ctgatgcgtc gtttgttgtc tgtcgcccct
    11761 gtccttctct ggctgattac gcccctcgcc ttcgcgcaac tgccgggtat caccagccag
    11821 ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc agacgctggt gttcatcacc
    11881 tcgttgacgt ttattccggc aattttactg atgatgacca gtttcacccg catcattatt
    11941 gtttttggtt tattgcgtaa cgcgctggga acaccctccg cgccacctaa ccaggtattg
    12001 ctggggttgg cgctgttttt gacctttttt attatgtcgc cggtgatcga caaaatttat
    12061 gtcgatgcgt accagccatt cagcgaagag aaaatatcaa tgcaggaggc gctggaaaaa
    12121 ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc gtgaggcgga tttagggttg
    12181 tttgccagac tggcgaatac tggcccgttg cagggacctg aagccgtgcc gatgcgcatt
    12241 ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat ttcagatagg cttcacgatt
    12301 ttcatccctt ttttgattat cgacctggtg atagccagcg tgttgatggc attagggatg
    12361 atgatggttc cccccgccac cattgctctg ccctttaaac tgatgctgtt tgtactggtg
    12421 gatggctggc aattgctggt cggttcgctg gcgcagagct tttacagcta gagaggcaaa
    12481 atgacacctg aatcggtcat gatgatgggg actgaagcga tgaaagtcgc gctggcactg
    12541 gctgccccgc tattgttggt agcgttggtc acgggcctta tcatcagtat tttgcaggcc
    12601 gccacccaga ttaacgaaat gacgctgtcg tttattccga aaatcatcgc cgtatttatt
    12661 gccattatta ttgccggacc gtggatgctc aatctgttgc tggattacgt cagcaccttg
    12721 ttcactaacc tgccgtatat catcgggtag ccgtactatg ttgcaggtga caagcgatca
    12781 atggctttcc tggctaagtc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc
    12841 taccgcgcca attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat
    12901 gatgatcacg ttcgctattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt
    12961 ctttgctctg tggctggccg tgcagcagat cctgatcggc attgcgcttg gtttcaccat
    13021 gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct
    13081 gtcatttgcg acgtttgtcg atccggccag ccatctcaat atgcctgttt tagcgcgtat
    13141 catggatatg ctgacgttac tgctgttcct gacatttaac ggtcatttat ggttgatttc
    13201 actgctggtc gatacctttc acaccctgcc aattggtggc gaaccgttga acagcaatgc
    13261 gtttctggca ctcaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt
    13321 accgctcatt actctgctgc tgaccctgaa tctggcatta ggtttactga atcgtatggc
    13381 cccgcaatta tccatttttg ttattggatt tccattaact ctgactgtcg gtatctcttt
    13441 aatggcggca ttaatgccgt taattgcacc tttttgcgaa catttattca gtgagatgtt
    13501 taatttgctg gctgatatta ttagtgaatt gccattaata taattccgta acgtttatca
    13561 tgttatccta aggattatcc gaaaaataat acctacgaac atcttccagg atactcctgc
    13621 ggcgaaatat ttgttttaag ctcactcaca tatcgcaaca tttactttac tttaagacaa
    13681 ttccaggcaa attatacaac actttacggg atagtaagtc cgcctgaaaa atcgcgagag
    13741 tggcgcatta ggtgacccat gttgttccgt ttagtcatga tgaaatattc aggtaagggg
    13801 aattatcgtt acgcattgag tgagggtatg ccatgtcaac gattattatg gatttatgta
    13861 gttacacccg actaggttta accgggtatc tgttgagtag aggggttaaa aaaagagaaa
    13921 tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg tgattcacag cgcccttcag
    13981 tggtgtttat taatgaggac tgtttcatcc atgatgcttc taacagtcag cacatcaagc
    14041 acatcattaa tcaacatccc aatacgttat ttatcgtttt tatggcaatt gccaatgttc
    14101 attttgatga atatctattg gtcagaaaaa atttattgat cagttctaaa tcgattaaac
    14161 cggaatctct cgacgatatc cttggcgata ttctgaaaaa agagacaacg ataacctctt
    14221 ttttaaatat gccgacgtta tcattgagcc gaaccgaatc gagtatgttg cgaatgtgga
    14281 tggcaggtca gggaacgatt caaatctctg accaaatgaa tatcaaagcc aagaccgttt
    14341 catcgcataa aggtaatatt aaacgtaaga tcaaaacgca taataaacag gttatctacc
    14401 atgtcgtccg actgacggat aatgtgacta atggtatttt tgtcaacatg cgctaacaca
    14461 ttctgactgg tggtttccca ccagtcaggc tgaataagat tactctgctt tctccacaaa
    14521 gataccgtcc tgatgccctg cttcattaaa gaaccagatg ccgagcgggt agtcttccag
    14581 cgaaaccagg tacattgtgc cttcactaaa ctcctcaact gccagtacca cgccaggacg
    14641 acgcggaccg ccatccgttt tgactgttac ccgatcattc accttcatcg ttttcctcct
    14701 gtggctttgt gccagtgtag aacaatttcg tttttctggc agcgccaggc gcgcgcgagt
    14761 gctgattttc tcgacggtct atacttaaga gatgccaggc ggacttaacg actggcggca
    14821 acaacagagt aacggttgcg aggaaagatg atgaaaaccg caaaagagta cagcgatacc
    14881 gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa tgaaattagc
    14941 gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg ttagcgtcga tggacgtgaa
    15001 tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga cttcagcgtc
    15061 tctgaagtga atcgttgaat gcacaataaa aaaatcccga ccctgagggg gtcgggatga
    15121 aacttgctta agcaagaagc acttaaaaaa ttcgttacac caggaaatct gatgtgttca
    15181 tcaccttatc cgcaattttt ttcgctgaca agaaaatatt cgcgatatga atgattaacg
    15241 ctacttatga atagaaatgt gacgtgcgtc accccttggt ggctctggcg tcagcgggat
    15301 cgctcccgaa aaaatcatct ttgataattt gtgctgtttt gagaatcatc ttggtaaggg
    15361 gagtactgcg cgactatgtt ttcaattcaa caaccactac tggtttttag cgatcttgat
    15421 ggcaccctgc tggacagtca tagttatgac tggcaaccgg ctgccccctg gctcagccgt
    15481 ttacgcgaag cgaatgttcc cgtcattctc tgtagcagta aaacttcagc ggaaatgctg
    15541 tacttgcaaa aaacgttggg gctacaaggg ttaccgctga ttgccgaaaa tggcgcagtg
    15601 atccagcttg ctgaacaatg gcaggatata gacggttttc cacgcatcat ctcaggtatt
    15661 agccatggcg aaatcagcca ggttttaaat acgctacgcg agaaagaaca ttttaaattc
    15721 acaacttttg atgatgtcga cgatgcaacc atcgccgaat ggacgggatt aagccgtagc
    15781 caggcggcgc tgacgcagct gcatgaggcg tcggtaacgc taatctggcg cgacagtgac
    15841 gagcgtatgg cacaatttac cgctcgtctg aacgaactgg gcttgcagtt tatgcagggt
    15901 gcgcgcttct ggcacgtact ggatgcctct gccggaaaag atcaggctgc caactggatt
    15961 atcgcgacct atcaacaaca attgtcaggc aaacgcccaa ccacacttgg cctgggcgat
    16021 gggccaaacg atgcgccctt actggaggta atggattacg cggtgattgt gaaagggcta
    16081 aaccgtgaag gggtgcatct gcatgatgag gatccggccc gcgtctggcg aacgcagcgt
    16141 gaaggaccgg aaggatggcg tgaagggctg gaccattttt tctccgcccg ttaagcgtta
    16201 tcgctcgcgc atacccgatt acgcccggcc tgtttagcga gataaagtcg acggtcagcc
    16261 agtgactgga gttgttcaaa atcataatca ccggtttcct cgctgctgct tacccccagc
    16321 gaggcactga tgcgtatcgt cgtactcttg gcgatcaaca tctctttttc atttaatttc
    16381 agtcgaatac gttctgcgac ctccgcagcc tccgtcagac tcgcgcctgg cagaatcaca
    16441 caaaactcct caccaccgac ccgcccggca acgtcctgcg cacgcaagga actgctaatt
    16501 aatccggcag catgagaaag aacacggtcg cccgcctgat gaccaaagcg gtcattaatc
    16561 gctttaaaat ggtcaagatc gacctgaatg acagaaaaag gatgttggtg cgtctgacac
    16621 aatttagcga gcggacgggc tttttcgaac agtgcgccac gattatataa acgggttaag
    16681 gtgtcgtgcc acgcctgcca ctgcaacgag ctttgcagaa catacatgtt gctgaccatc
    16741 cggcgaatca cataccagga gatgagtaac atggtggtaa agagcgccca cagcagggtt
    16801 aatgcaatgc tgatactacc gaaatcgccg cgcacgcctt cgcttagcgt atggacgcgc
    16861 accagcacac cgtcgaaatg gtccagacgt tcccagctaa catagcgact gtccatacga
    16921 atgccgcccc gcgtgtcatg ttccatcgcc tgcgccagca aggccagttc acgaggatca
    16981 aaaatattcc ctgttggatg gtcaggattg gaagaggtca aaaatctcag cttactgtca
    17041 tagagctgat actcaccatc gaggttttta tcgatggcgt ttcttaaaaa ttgctgcata
    17101 gtacgtacgg gaatactcat ccccagcacg ccataccagt agttattact gtctaccgga
    17161 acactgacgg taacctgcgg ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag
    17221 cgtaccgcgc ggtgacgatt ttctcgttgt gaatggccga taaaccaggg ttgggtgaca
    17281 tagccgtaat aacgcgttgg aacattgcgc gtaaacaagg tcggctgcgt cgaaacgtaa
    17341 aatccggcac gcgagacata catcgcctgt tcaaccatcg acgaggagtt gtgcgccagt
    17401 cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat tgtcgaggct ttcattttcg
    17461 cgagacagga gatttccctc gctgactaat gcatccgata cgccattgac tggcagggtg
    17521 cgtcgtcggt tgagttcgat tttccaggcg tgctcgtcgc gatgctgttc gaactcggtt
    17581 acagcatcac gcagagaggt gaaatccagt ggcgctacga gagcttcgcg catgccatta
    17641 cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct tatcgagcgc gttggctacg
    17701 ttttccagat gattacgctg gctggagata taggcatctt ccagcaccac tacttcacgc
    17761 caggtgagca aggtggaaaa aagcaatacc acgataaagc agagattaac gacatgacca
    17821 ggccccaggc ggcgtgcgag ttttttcaac cagctctggt tttccatttt tgtctcgtgc
    17881 tgcaccctga cgactcccaa cctgcttttt tatgattctg gcataacctg gcgatagcgt
    17941 accgcaaaac agcgataatt ttataatggt ggcatgataa gacgcgacta tcattcattg
    18001 caggcattgg cacaccaggc cggatgtgat ccggtctggt gttaatgata ccctttcaaa
    18061 cttcttccgc aggacgactc ccctcccctg gaacaagctc ggtttcatga aaagcttccc
    18121 gcttgacccc gtaaccgtca taccaacgac actcaaccat accgctggag tatccagtga
    18181 caatcatccg cgggccgccc tctttaaccg taacttcctc actaaccata aagctcatac
    18241 tcgcctcctt ttttcgagtg aaacttgttc accttagttg aagatggcga attttgcata
    18301 atagcctgtg cgagtattaa tgtgcctgac cgcgcatttt cgctacggct ttcactccca
    18361 gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc ggtaatatca
    18421 tcgccaccac tgcactttgc tgcccggcga aatgttcaat ggcgtgatgc agcggcgcaa
    18481 taccatgtac cacaatcccg ccgccgacga ggaacatcgc cagcgtgccg acaatcgata
    18541 acgctttcat cagccagggc gcaatgatca ataacccttt acctaatgcc tgcatcagcg
    18601 cgctggattt ttccgccagc caatacccca ggtcatcaat cttaacgata acccctacca
    18661 gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc acctgattaa
    18721 gcaacggcgc ttctgccaca atccccagcg tgatggcgac gatttcggca gacaatataa
    18781 aatcggtacg aatcgcccct tttattttgt ccttttcaaa cttcagcgga tcctgcgccg
    18841 ccagtttctc cagacgctgc tggctctgcg ccggatcttc tttatgttta cgcgcctcca
    18901 gcatatgcag cactttctct accccttcaa agcagagaaa cgcgccacca atcatcaaca
    18961 gtggcgtaat cgcccacggg ataaacgcac tgatgatcag cgccagtggc accagaatca
    19021 ctttattgat cagcgatcct ttcgccacgc cccagaccac gggaagttcc cggttggccc
    19081 gcacgcctga aacttgttgc gcattgagcg ataagtcatc ccctaataca ccggcggttt
    19141 tcttcgccgc cagtttgccc atcacggaga tatcgtccag cagtgtggcg atatcatcga
    19201 gcaacgtcag taaactactc ccggccaaaa gcattccctc tcatttttat tgttgaaggg
    19261 tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa cattttttta
    19321 acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta ctatgaacca
    19381 cctttttatt tccaccgtga gggattatgc gtttccgcca gttgttaccg ctttttggcg
    19441 cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg attggcgtgg
    19501 aaagctggcc gccgttaatg atggcgggcg tgcgattcct ggcagccggt attttattgc
    19561 tggcattttt gctactgcgc ggacacaagc tccccccgct acgtccgctg ctcaatgccg
    19621 cgctgattgg cctgttattg ctggctgtcg gtaatggcat ggtaacggtt gccgaacatc
    19681 aaaatgttcc ttccggcatc gccgccgtag tggttgcaac cgtgcccctc tttaccctgt
    19741 gcttcagccg cctgtttggc attaaaacgc gcaaactgga atgggtgggt atcgccattg
    19801 ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat ccgtggggcg
    19861 cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcagtttat ggctcgcgca
    19921 ttaccttacc tgtagggatg atggcgggtg cgattgagat gttggcggca ggcgtggtgt
    19981 taatgatcgc gtcgatgatt gcgggtgaaa aactgacggc gctcccttcc ctttcaggct
    20041 tccttgcggt tggctatctg gcgctgtttg gttcgattat cgccattaac gcttatatgt
    20101 atttaatccg taatgtcagt ccggctctcg ccaccagcta cgcctacgtt aacccggtgg
    20161 tcgcggtctt gctgggtacg ggactgggcg gagagacgct gtcgaagatt gaatggctgg
    20221 cgctcggcgt aattgtcttc gcggtggtgc tggtcacgtt gggaaaatat ctctttccgg
    20281 caaaacccgt agttgcgcca gttattcagg acgcatcaag cgagtaaatg aatcccctgc
    20341 gtgtcgatct gcgcgctagc accttcgccg cagatccact cttccagacg ctcgctaagc
    20401 gcttcatccg tcagcttctc gcgcccacgt aacgcgcact cccagacaat caatacgcgc
    20461 cagccgagtt cctgcaagcg actgatatcg cggcgatcgc gctcaacatt tttacctatc
    20521 ttctccagcc agaactcggt tcgagtcgca ggcactttaa acagatagca gtgatgatga
    20581 tgccagaagc agccgtgggt aaatatcacg cagcgatatt catcaacgac aaaatccggg
    20641 cgtccgggca gactggcgtc ctgaacgcga aacgccaggc cctgcccggt taacagactg
    20701 gcgaggcgct tctctatcgc cgtatcacgc gtggcaatcg cgcgcatatt tttgctgcga
    20761 gtggccttat cgtgaacgtc ggccatgttg tgcctcttgc tgacgcaacg ccaccgcctg
    20821 tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga ccaccgagtt
    20881 accgaactgg cgataggcct gagtgtccga aaccggaatg cggaatttcg cttctcccgg
    20941 cgcttcaaaa cccattaagc gcgcgcattc acgaggcgtt aaccgacgtg gacgatgttg
    21001 ctgattctgc ggatcgtcaa agtctttctc acctttggcc atatcccagc cgcgatcgat
    21061 taaaatttcc gcgccatctt tgtaataacg cgcagacagc gtacgcgtga cgctttgcgg
    21121 attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat gttttttcgc
    21181 atatcgatag aggtacttcc acagcaccgg cgtcaggata tatttcgcct cgaccatcgg
    21241 gtccagcagc tgcgccagcg tcactcgctg cgcagggaaa cattcgctga tatcacgcag
    21301 ggtaaaatcg gctttcagat tcagatcgcg acgaaaaccc accagcacga tgcgttcacg
    21361 gtgctgcggc agaaaatgtt tgccgtcgat gattttcgga tcgtctggcc cgttatcttc
    21421 tgcatcagcc acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc ggaacgtttt
    21481 accctggtcg tgacttttca ggtttttgac gttttcgagc acaaacatcg ccggacgacg
    21541 cgcgtcgata atgcgtacca catcaaaaaa cagcgtgccc tgggtatcgc aggcaaaacc
    21601 gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg gctgacaagg
    21661 gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt ccgccgccgc
    21721 ctcatcactc acgccttctt tatggctgag ggtgatgtcg cggatatctt cattaaaatg
    21781 atgcgtcgcc gggtcgcaat aatgattggc tttataagtg cgtaccgcat gtttgttcca
    21841 ttcgctggta aacacgcact gtccgccaat cgattcaaaa ccgcgacgga tgccgccaat
    21901 tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgtgccg gtggtttggg
    21961 taacaacgtt tgcagatggg cgaactcttt ctcacttaaa cgatgccatg ccgagtcatt
    22021 cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac cattaagctg
    22081 cgccaccagc gttttaacat cataaatttg cagcagtttc tccagcattg cctgtgcggc
    22141 attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt taaccggcca
    22201 tctgagatgg ccgacaggtt aacataatta cagccattta gggccacgcg cttatcccgc
    22261 gggtgaaaac gcatctatca ccttgtgatc gacaccttca ttctcccccg ccagttcggc
    22321 actgagcttc gccataaact cgactaaaaa atgcgcgttg tgctgcgcca gctgcttgcc
    22381 tcttatggtt tgcatggtat gcggtagctt cagcaattta gtctggaaat gatccagcgc
    22441 gtagcgttta tcatcaagcg ggcgatgctg tgcgaacgga tcttcaccat caaacagcgc
    22501 cacgcccaat gctcctgaaa cggcaaacac gcgcgccagg ccaatcgccc ccagggcttc
    22561 cagccgatcc gcatcctgga caattttagc ttccgtggtt aagggggcga tttgcgcgct
    22621 gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg gaaattgcac
    22681 gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag aacgctgccg
    22741 ttgcggatga tttttcgcca ggctcacaat atcgtgaaaa taacaggcgg ttaaaatcac
    22801 cagcatatcg acgtcatcat ctgccgccag cttctgtgcc gtagcccaga cgcggcgaaa
    22861 atgacacacg tcatgggccg cgtcctggtg ctgatgatgg tttttcaacc agttttcgaa
    22921 ctctgcctgc cagtgttgta agtccatacg cgctccgttg ccaaagagtc gccaccttag
    22981 cagtttttat tcatagcgtc tgctacgtgc gggacgaggt cgggtgtacc aggcaatgct
    23041 gcctaacaat gccccgacca gagccataaa taaaaatccg accagtgccc ctaaccactt
    23101 tcccgcggta gagcctaaat cgctttcaaa tcccgacacg ggggcatcgg ggatccggct
    23161 catcacccag atgtaacgcg ccattgccca gacgaaatag ccacaccatg tatagaaagc
    23221 ccacaaagcc agtttgccac cagggctgcg agagagcctc tcttccatct ttgtcgaacc
    23281 gtattccatt ttgagataac ccggaaaatt cagacttagt actattatgt gataaaagtc
    23341 acatttttcc acattgaatg agccagtcaa gactcaatca ttttttgaac aaatagataa
    23401 attttgcgca acggcagtga atacaggaaa aatcgttttg agcgatttta tttatctttg
    23461 atatcggctt tattaattc
//
LOCUS       DAZFFI010000069        23431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFFI010000069 DAZFFI010000000
VERSION     DAZFFI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.069"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(75..395)
                     /gene="chbB"
                     /locus_tag="ACHJ12_003162"
     CDS             complement(75..395)
                     /gene="chbB"
                     /locus_tag="ACHJ12_003162"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4990913.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(694..1032)
                     /gene="osmE"
                     /locus_tag="ACHJ12_003163"
     CDS             complement(694..1032)
                     /gene="osmE"
                     /locus_tag="ACHJ12_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4990914.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1234..2061
                     /gene="nadE"
                     /locus_tag="ACHJ12_003164"
     CDS             1234..2061
                     /gene="nadE"
                     /locus_tag="ACHJ12_003164"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4990915.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2291..3178
                     /gene="cho"
                     /locus_tag="ACHJ12_003165"
     CDS             2291..3178
                     /gene="cho"
                     /locus_tag="ACHJ12_003165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4990916.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3138..3713)
                     /gene="ves"
                     /locus_tag="ACHJ12_003166"
     CDS             complement(3138..3713)
                     /gene="ves"
                     /locus_tag="ACHJ12_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4990917.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3916..4401)
                     /gene="spy"
                     /locus_tag="ACHJ12_003167"
     CDS             complement(3916..4401)
                     /gene="spy"
                     /locus_tag="ACHJ12_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4990918.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4731..5699)
                     /gene="astE"
                     /locus_tag="ACHJ12_003168"
     CDS             complement(4731..5699)
                     /gene="astE"
                     /locus_tag="ACHJ12_003168"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4990919.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAKDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5692..7035)
                     /gene="astB"
                     /locus_tag="ACHJ12_003169"
     CDS             complement(5692..7035)
                     /gene="astB"
                     /locus_tag="ACHJ12_003169"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4990920.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQAQLLAN
                     LRARVNSFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7032..8510)
                     /gene="astD"
                     /locus_tag="ACHJ12_003170"
     CDS             complement(7032..8510)
                     /gene="astD"
                     /locus_tag="ACHJ12_003170"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4990921.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHCPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8507..9541)
                     /gene="astA"
                     /locus_tag="ACHJ12_003171"
     CDS             complement(8507..9541)
                     /gene="astA"
                     /locus_tag="ACHJ12_003171"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4990922.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAVQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9538..10758)
                     /gene="astC"
                     /locus_tag="ACHJ12_003172"
     CDS             complement(9538..10758)
                     /gene="astC"
                     /locus_tag="ACHJ12_003172"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4990923.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11204..12010
                     /gene="xthA"
                     /locus_tag="ACHJ12_003173"
     CDS             11204..12010
                     /gene="xthA"
                     /locus_tag="ACHJ12_003173"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4990924.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12177..12887
                     /locus_tag="ACHJ12_003174"
     CDS             12177..12887
                     /locus_tag="ACHJ12_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4990925.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12892..13569
                     /gene="ydjY"
                     /locus_tag="ACHJ12_003175"
     CDS             12892..13569
                     /gene="ydjY"
                     /locus_tag="ACHJ12_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4990926.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13584..14291
                     /locus_tag="ACHJ12_003176"
     CDS             13584..14291
                     /locus_tag="ACHJ12_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4990927.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14291..14839
                     /locus_tag="ACHJ12_003177"
     CDS             14291..14839
                     /locus_tag="ACHJ12_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4990928.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14849..16015
                     /locus_tag="ACHJ12_003178"
     CDS             14849..16015
                     /locus_tag="ACHJ12_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4990929.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15988..17523
                     /locus_tag="ACHJ12_003179"
     CDS             15988..17523
                     /locus_tag="ACHJ12_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO4990930.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17523..18176
                     /locus_tag="ACHJ12_003180"
     CDS             17523..18176
                     /locus_tag="ACHJ12_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4990931.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18243..19550
                     /gene="ynjE"
                     /locus_tag="ACHJ12_003181"
     CDS             18243..19550
                     /gene="ynjE"
                     /locus_tag="ACHJ12_003181"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4990932.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19559..20179)
                     /locus_tag="ACHJ12_003182"
     CDS             complement(19559..20179)
                     /locus_tag="ACHJ12_003182"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4990933.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20266..20673
                     /gene="nudG"
                     /locus_tag="ACHJ12_003183"
     CDS             20266..20673
                     /gene="nudG"
                     /locus_tag="ACHJ12_003183"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4990934.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20639..20911)
                     /locus_tag="ACHJ12_003184"
     CDS             complement(20639..20911)
                     /locus_tag="ACHJ12_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4990935.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21147..22490
                     /gene="gdhA"
                     /locus_tag="ACHJ12_003185"
     CDS             21147..22490
                     /gene="gdhA"
                     /locus_tag="ACHJ12_003185"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4990936.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22607..>23431)
                     /locus_tag="ACHJ12_003186"
     CDS             complement(22607..>23431)
                     /locus_tag="ACHJ12_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4990937.1"
                     /translation="GCSFLYLLIMMGLIVRAGFKVKKEQLHYYQAKGIEPLSIEKLQA
                     LQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGI
                     EDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSG
                     KSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNS
                     LMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5599 a         6202 c         5961 g         5669 t
ORIGIN      
        1 ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga
       61 aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc
      121 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
      181 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
      241 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
      301 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
      361 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
      421 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
      481 caacatgact ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt
      541 ctttgacctt gtaaattgtc tctccttgac ctgcggttat gaccctcttc acatttcggg
      601 caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt
      661 ttccgttgtt actcaagctt tcagacgaag tgattacttc gcagcctgtg gatcagtgtc
      721 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
      781 aaagtaggtt tctgctttac catcacgttg acccagaatg taggtctggc aagtaccacg
      841 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
      901 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc
      961 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
     1021 attcttgttc atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt
     1081 gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
     1141 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa
     1201 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
     1261 gcgctgggcg caaaaccgca gattaatgcc gaagaggaaa ttcgtcgtag tgtcgatttt
     1321 ctgaaaagct acctgcgaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
     1381 ggtcaggact ccacgcttgc cggtaagctg tgccagatgg cgattaatga gctgcgccag
     1441 gaaaccggca acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc
     1501 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
     1561 aatatcaagg gcgcggtatt ggctagcgag caggcattgc gggaagcagg cattgaactg
     1621 agcgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
     1681 gcgggtatga ccagcggtgt cgtggtgggc accgatcatg cagcagaagc cattaccgga
     1741 ttcttcacta aatatggtga cggcggtacg gatattaatc cgctgtatcg tctcaacaaa
     1801 cgccagggta aacagttgct gacggcatta ggttgcccgg aacaccttta taagaaagcc
     1861 ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg
     1921 acctatgaca atatcgacga ctatctggaa gggaaaaacg tacctgaaca ggtcgccaga
     1981 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
     2041 gatgatttct ggaaaaagta ataatttgca cataatggtt tgtgcgaaaa agagtaattt
     2101 gttcacgccg gatgcggcgt gaacgcctta ttcgacctac aaaactatgc aaattcaata
     2161 tattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca
     2221 tcgacccgcc tcttttttaa tcgcttcccg cctgttacac tggatagata accagcattc
     2281 ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat
     2341 ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt
     2401 gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcattaacat
     2461 ccgcagccgc gtcctttctc atttacgtac cccggatgaa gccgccatgc tacggcaatc
     2521 ccgacgcatc agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg
     2581 attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg
     2641 tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc
     2701 acgagccccc aacctgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca
     2761 gagcatcgct gatgaacaaa aactttgtta tggcctgttg ggactggaac cgttaagccg
     2821 cgggcgtgct tgttttcgtt ctgcattaaa acgttgcgct ggggcgtgtt gcggtaaaga
     2881 gagccatgag gaacatgcgc tacgcttgcg ccagtctctg gagcgtttgc gggtggtgtg
     2941 ttggccttgg caaggggcgg tggcgctgaa agaacagcac ccggaaatga ctcaatatca
     3001 tattattcaa aactggctgt ggctgggggc ggttaattcg ctggaagaag cgacaacgtt
     3061 aattcggaca cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct
     3121 ttccggtaac tatgaaatta ctgaacttga tccggcgaat gaccagcgag ccagttgatt
     3181 tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac
     3241 cagcacgcgc cctggtcggt agtgagcagt ttgtcgccca gttgccacgc cccgttaatc
     3301 acaaacacca ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact
     3361 ttcgccttac agacatccag gcgcgtcata atattgaaat ccatcgacat ctgccctgcc
     3421 gtcagtttcg cttttaccac ctggtccgcc gcaaaggcaa aaggctgtaa cggttttaag
     3481 gtatggttaa agcggtctgt gctttcaagg aacatctcgc cgccttcaag caacgtcact
     3541 atcctttcca tgcctggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg
     3601 gcacgccagt aaaaatcacg ttttgccggt gggaacgtgc aaatttcgcg cgtttcaccg
     3661 gcagcgtttc gccacagatt caccgacatt ttacgcatat caaagtattc catactcgct
     3721 cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg
     3781 ggtgatttac gttagtggtg atcaggcttt ttttgcatct ctttgagaac aaaaaagaaa
     3841 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
     3901 tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc
     3961 tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt
     4021 gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat
     4081 ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg
     4141 cattgcgcgg cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat
     4201 ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg
     4261 atgcggaccg aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgcggt
     4321 agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccagggtag aggcaacaaa
     4381 cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat tacggctttc
     4441 ttaagtagcg tgccgtgttg acgagattaa ctttactgac tttagcgtca attaatcaga
     4501 gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag
     4561 aaaatttgat ggagagtgat gagagaatat tacaacacca tgattttgca gagattatga
     4621 agaactacac cggatgactg gtgataaata aagcaaataa ccaggattaa tctgtattaa
     4681 tttataagaa agcaacttaa tacccgcaga atgatttctg cgggtaagta ttagcttatt
     4741 ttttcgagca ttaatcccgc gcgtaatccc aacgctacca acggattagg gaataacaca
     4801 tactctacat catgggttac ggtaaaacgt tcctcaccgt ccttcgccag caacgttcct
     4861 ttcttaaacg gcataaaatt cagcgtgtca cttgccatat gcatttcgaa ggacggcgag
     4921 tggcgagtaa tttgcgaaac cacccgataa cggagcggcg gtgttctcac gataccgaca
     4981 ctctcaccag atagcagcgc agcaattgcg ctggcagtta ctgcaaactg gcgaagatcg
     5041 ttttgcccaa agggcaacgc tttgccaagt tccagcgtac aggccagcgc gccaaaatgt
     5101 ctggcgctga aatgggtaaa cgtaccacca ggctcctgat ggaacaccag cgcctccagc
     5161 cccgccgcac ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat
     5221 acaccgaact gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccagcgc
     5281 acagattctt tgccctggtc ataaaaatct tccaggcact gttccagttc gcgcgcccga
     5341 caggtttctc cgctttcagc aaacagctgc caacgaccgc caaacattcg gttcatatcg
     5401 ctatggcaat aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagcaac
     5461 cgccaccgta acgggatctc gccgtgagat atcgcgccaa gcaatgcatc aagcatctcc
     5521 acaggtgccg tctcattacc gtgtattccc gctgaaatca ccagtgcgcc ttgcggtggc
     5581 gtcaatggcg tcagttccag cacaccatcg cctagccagc gccagcgaac gccgttgatt
     5641 tctcgctcgg tgataaccgg ttttttaccc gttaaggtca gagcaagaaa attatccatt
     5701 cccccctccc tcgcgctgga acggataaac cgaaccgaga ttcagtaatt gactcaatac
     5761 atccagtgct tcccgccctt cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag
     5821 acgatcgcgg tagtaacgat ccacccagtc attgagcgca ttaaacaagg tatcgttcat
     5881 catcaccgcc ggattcaccg cccggcgttc ttcttctgtc aataccaccc gcaaccgcag
     5941 gcacgccgga ccgccgccat tcgccatgct ttcacgtaaa tcaaagactt ttagttcgct
     6001 aatcgggttg tcagcggcaa ggagttcatt gagataaccc catactccgg cgtgttcccg
     6061 acactcctga ggcagcacca acatcatgga accatcatcg cggctcagca gttggctgtt
     6121 gaacagatag gtagacaccg catccgacac ggaaacctgc gccgtgggca cctctattgc
     6181 cataaagcta ttgacccgag cacgcaggtt tgccagtaac tgcgcctgtc gagcaaacgc
     6241 ctgttgatga caaaacagca cctggcggtt actcacggca atcacgtcat tatgaaaaac
     6301 gccctggtcg ataacgtccg ggttttgctg ggcgaaaatc acctgttggg gattcacctg
     6361 attcagcctt gccaccgcct cgctggcttc gcgagtctgt cgcgccggat atcgggaagg
     6421 ccgggtatca ttgccttctt ctcgcccgta gacaaaaagt tgcatacccg gctcaccgta
     6481 atgaccgccg agacgattgt ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg
     6541 caacgccgaa tgaacgctaa atttctcttc gtcgttaaaa atcgctttta aaagtgattc
     6601 ggtgacgtgc gcttccagcg agcggtgaaa tttattgttc aggttggcaa cggtgagatg
     6661 cactttgcca tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg ctacccacat
     6721 tggcgaagcg gaactgacgc tggaaagcca gtgcggtgcc tggcgtgcaa ctttttccag
     6781 tacctgctca tcgctaccac tgaatcccaa ctgacgcagc accggaataa acggacgctc
     6841 gtgcggcggg atcacggcct gggggaatcc cgcatcggca agggctttca ttttcagtaa
     6901 gccctgtttt gccgcctgtc gcgggttaga cacctgaaaa cggtgacggg tagaggcttc
     6961 attaccaaac gacaggcccg cgtaatgatg cgtcagccct accagcccgt cgaaattaac
     7021 ttcccaggcg ttcatcgcac cacctcatcg gaaaaatcca gcccggggtt aagcgttgcg
     7081 ggcaaagtta acgagtccga ctccaggctc gccataggcc atgcgcagta atctgcggca
     7141 taccaggcgc tggggcgatg gttaccggat gcaccaatgc cgccgaatgg cgcggtactg
     7201 gcagcaccag taagcggttt gttccagtta acaatccccg cccgcgcctc cagcaacagt
     7261 tgatcgaatt tttcccgctc gggggaaacc agaccgcaag agagtccgaa gcgagtgtta
     7321 ttcgccatta gaatcgcttc ctcgaaagaa tcataacgcc agacgcgcaa taacggtccg
     7381 aacacctctt catctggtac gccagcaacg cctgtcattt caatgatccc cggcgtcagc
     7441 aacgatgtct ctgattgtaa taagcgcggc gcaagcaggg ttcgtccgcc catcgcttcc
     7501 agttgctgcc aggcagtaac cacctgctgt gcggcctgtt cagaaatcag cccgccaata
     7561 aacggctgcg gttcgtcatc ccagttgccc ggcgttaatc gctggctgac ggcaaccaga
     7621 cgagcaagaa acgcatcgcc ctgcgctccg cttttgagca ataaacggcg ggcgcaggtg
     7681 cagcgttgcc cggctgtgac aaacgccgac tgaatggtca gatggacagc cgcgtcgata
     7741 tccgccacct catcgataat tagcggatta ttaccgccca tctcaagggc gagaattttc
     7801 tccggctgac cggagagctg gcgatgcagc tggtagccgg tattggcgct accggtaaac
     7861 agcaaaccgt cgagatcctc cagcgcactc agcgcctgac ccgtttcacg cccgccctgc
     7921 accaggttca gtacgcccgg cggcaagcca gcctgctgcc ataaacgcat taccgcttcg
     7981 ccactccacg gtgtcagttc gctgggttta aagataatgg tgttacctgc cagcaatgcc
     8041 ggaacgatat gtccgttcgg caaatgacca gggaaattat acggcccaaa caccgccagc
     8101 acgccgtgcg ggcaatgtcg caggctcgcc gcaccgtccg gcatttcact acgctgctcg
     8161 ccggtacgaa cgtgatacgc cttaattgat atcgcgattt tattgatcat cgccgtcact
     8221 tcggttgccg cttcccagcg cggcttaccc gtttctctgg caataatcgc ggttaattcg
     8281 cctttattcc tttccagcaa tccggcaaag cgttcgacaa cgacctgacg ttcagccaat
     8341 gagagccgcg cccagcgcgg aaacgccgca cgggctgccc gacaagcctg ttcgacctga
     8401 gcggcatcgg catcattgcc ttgccataac acctcgcccg ataccggatt acgcttcaca
     8461 cgcgatgcgc cctggcccgt tatccagtca ccgttaatcc ataaagtcat gctgttttct
     8521 cctctgcgca caggcgcacc agacgaacgc gatcccctgc gtggcatttg agggcatcca
     8581 gttgtacggc ggttaaaatc aaacgctcgg ttgccggatc ggtacgcgcc agcaccacgc
     8641 ggaaatggtg ataattttca ttggcgacca ggcaggccgg aaaatcgccc tgcgcaggct
     8701 gcccttctgc cacttccacc agccgacttt tacgaatggc gcgcacgcgg tcgatgtcac
     8761 actcaagcgt tggcccgccg tcgaagatgt cgatatagtt gcggtaacga aaaccttctt
     8821 tctccagcac cgcgcgggca ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg
     8881 cttcctggga taaaaagtgg gtatagatcg gatgtttcgg catcagttct gcaataaatg
     8941 ccttttgccc ggtgccgcag agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt
     9001 taccgaggct ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag
     9061 caaccacttt gtcattaaac ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg
     9121 acagcaaata gccgttgccc tctttgcgcc agtccgggtc gagaaacagc gtgcacagct
     9181 cgctgctgcc ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta tagacattca
     9241 gctcttttga ggcgtgaacc aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc
     9301 caaccgccac ctcaatggca caaatccccg ccacggtgcc tgtctcgcta tcttccagca
     9361 cgaacacata gccctgctca cttttgggca gttcgccttg ccaggttttg attgcccttt
     9421 cgatacgcgc cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt
     9481 tgctggcaag ctgcatcagc gccgagacat ctgatcgctc aacgggacgg atgaccatca
     9541 tgatgaacct cggctaacaa agtgttcgca agcagctgca aagcgatcca gtccggtcgt
     9601 cacctcttct tcgctgacat tgagcgcagg cgcaaaacgc accacgttgc cacccgcaat
     9661 cagtaccatc acgcctgctt tcgccgcttc ctgagagatc tgtttcgctt gcccggcgta
     9721 atcggcattc agtacacagc caatcagcaa acctaagccg cgaacttcac tgaacaaacc
     9781 atagcggtga ttaatagtat taagacgctc aacaaaccag tcgtgacgct gtttaacgcc
     9841 attaagcatc tctggtgtgt tgatgagctc cagcactttg cctgccaccg ccgaggccag
     9901 cggattaccg ccataggtgg tgccatgagt gccaacggtc atcacgcggg cgcactcttc
     9961 ggttgccaac aacgcaccga ccgggaaacc gccgcccagc gctttggcgg tagttaacag
    10021 atcaggcgtc acgccgtagt gcatataggc atacagttcc ccggtgcgcc cgacgccggt
    10081 ttgtacttca tcaaaaatca acagcgcatt gtggcggtta cacaattcac gcagaccttg
    10141 taaaaacgcg ttgctggctg gcaccacacc gccttccccc tggatgggtt cgacaatcac
    10201 cgcacaggta gagtcgtcaa tcagcgcgct ggcagagtta atatcgttat atgcagcatg
    10261 acgaatatcc gccggcagtg gcgcaaaatc ctgtgaatag gctggctgcc cacccgcact
    10321 gacagtaaac agcgtgcgac catgaaacgc atttttgaac gccacgatgc cgctcttatg
    10381 gctgccgtag cggtcgtgag cgaatttacg cgccagtttt agcgccgctt cgttggcttc
    10441 cgcaccggag ttacaaaaga agacgcgatc ggcaaacgtg gcgtcgatca attttttcgc
    10501 cagtcgcagt accggctcgt tggtgtaacc gttgccggta tgccagaact tactcgcctg
    10561 ttcgttcagc gcttcacgca gttccggatg cgcatggccc agcgcgttca ccgcaatgcc
    10621 acccgcgaag tcgatatact ctttcccctg ctgatcccac aagcgcgaac cttcgccacg
    10681 taccggtata aagggtgccg gagcgtaaac aggtatcatc cattcatcaa agttttcacg
    10741 cgtaattggc tgagacatag cgacctctac agtaaataat tcgttattta tatgttaaca
    10801 ataagtaatg tttgcgctgt aaatgtagat tgcagggttc gtgccagcca gtgataaaag
    10861 tgcataaacg gtggaggcta actggaaatc aaggagttat aaccaaacca tatgcattta
    10921 aagtgcatat aaagtgaata cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa
    10981 tgttatgcag aagtaaaata taattctgga attgtgatca ttgacgaaat ttactggaaa
    11041 ttactgcgcc attctgacgc agcgcgcacc aaaagcgggc attttttgcg ccatcgttga
    11101 catcattaac aaccatcgat caaatcactt aacaacaggc ggtaagcaac gcgaaattct
    11161 gctaccatcc acgcactctt tatctgaata aatggcagcg actatgaaat ttgtctcttt
    11221 taatatcaac ggcctgcgcg ccagacctca ccagcttgaa gccatcgtcg aaaagcacca
    11281 accggatgtg attggcctgc aggagacaaa agttcatgac gatatgtttc cgctcgaaga
    11341 ggtggcgaag ctcggctaca acgtgtttta tcacgggcag aaaggccatt atggcgtggc
    11401 gctgctgacc aaagagacgc cgattgccgt gcgtcgcggc tttcccggtg acgacgaaga
    11461 ggcgcagcgg cggattatta tggcggaaat cccctcactg ctgggtaatg tcaccgtgat
    11521 caacggttac ttcccgcagg gtgaaagccg cgaccatccg ataaaattcc cggcaaaagc
    11581 gcagttttat cagaatctgc aaaactacct ggaaaccgaa ctcaaacgtg ataatccggt
    11641 actgattatg ggcgatatga atatcagccc tacagatctg gatatcggca ttggcgaaga
    11701 aaaccgtaag cgctggctgc gtaccggtaa atgctctttc ctgccggaag agcgcgaatg
    11761 gatggacagg ctgatgagct gggggttggt cgataccttc cgccatgcta atccgcaaac
    11821 agcagatcgt ttctcatggt ttgattaccg ctcaaaaggt tttgacgata accgtggtct
    11881 gcgcatcgac ctgctgctcg ccagccaacc gctggcagaa tgttgcgtag aaaccggcat
    11941 cgactatgaa attcgcagca tggaaaaacc gtccgatcac gcccccgtct gggcgacctt
    12001 ccgccgctaa tttagcagct ctcctggctc aaactgggtc aggagaatta accttgagaa
    12061 aaatcaacaa actgtcagta atgatttgtt gcctgccgtc ctttgttata ccgtcactgc
    12121 gtttttagtt gtctgaccac ttctctatta tcaagtttga tataggaaac tccacgatga
    12181 acgctgagcg taactttctg tttgcctgtc ttatttttgc gctggtcatt tacgctatcc
    12241 acgctttcgg tttattcgat ctgctcaccg atttacccca cttacagaca ctcattcgcc
    12301 agagcggatt gttcggttat agcctctata ttctgttatt catcattgcc accctctttc
    12361 tgttaccagg aagcatactg gtgatcgctg gaggaatagt ttttggcccg ctcttaggga
    12421 cactactctc attaattgcc gcaacgctgg cctcctcgag ctcattcctg ctggcgcgct
    12481 ggctgggacg tgatttactg ctgaaatacg tgggccatag ccataccttc caggccattg
    12541 aaaaaggcat tgcgcgtaac ggtattgatt ttcttattct gacccgctta atcccgttgt
    12601 ttccttacaa tattcaaaat tacgcttacg gattaaccac catcgccttc tggccttata
    12661 cccttatttc ggcactcacg accctgcccg gtattgttat ttataccgtg atggcaagcg
    12721 atctcgccaa tgaaggcatt acgctgcgct ttattttaca actctgtctg gcgggcctgg
    12781 cgctgtttat tctcgtccag ctcgcaaaac tctacgcccg acacaaacat gtggatctgt
    12841 ctgcttcgcg ccgcagccca cttactcacc caaaaaatga aggatagaac gatgtcgcaa
    12901 cattattcag tgtcatggaa aaaaggactg gctgcactct gcttactggc tgttgccggg
    12961 cttagcggct gcgatcaaaa agagaatgcg gcgggaaaag tggaatacga cggactttcg
    13021 aacagccaac cgctgcgcgt cgatgccaat aaccatacgg taaccatgct ggtgcaaatt
    13081 aatggtcgtt tcctcaccga cgtcactcgt cacggtattg tgtttaaaga tggctccaac
    13141 ggacataaat cgctgtttat ggcttatgcg accccgaaag cattttatga agccctgaaa
    13201 gaggcaggtg gtacgccggg cgaaaatatg acgatggata ataaagaaac gactcttgtc
    13261 acaggcagca aactggatat ttcggtcaac tggcaagggg cggcaaaagc gtattccttc
    13321 gatgaagtga ttgttgacag taatggcaag aaactggaca tgcgctttgg cggtaattta
    13381 acggcagcag aagagaagaa aacgggttgc ctggtgtgtc tggatagctg cccggtcggc
    13441 atcgtcagca atgcaacata cacttatggt gcggtggaaa aacgcggcga agtgaaattc
    13501 aaaggcaatg cctcagttct cccggcggat aacacgctgg caacggttac ctttaaaatc
    13561 accgaataaa gccaggataa aggatgatga agatacaatc gcgtaaaatc tggtactacc
    13621 gtataaccct catcatcctg ttgttcgcta tgctgctcgc atgggcgctg cttcccggcg
    13681 tccatgagtt tatcaatcgc agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag
    13741 aacgctttat tcagtcttac ggcgcactgg cggcagttgt ctcgttcttg ttgatgattt
    13801 tacaggccat tgctgcaccg ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt
    13861 ttggcgcgtt ctggggcggc ttgctgtcgt ggaccagttc gatggccggc gcggcgctgt
    13921 gcttttttat cgccagagtg atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg
    13981 tgcttgacag tatggacggc tttttcactc gctacggcaa acacaccatc ctggtctgtc
    14041 ggttattgcc ttttgtccct ttcgatccaa tcagctatgc tgccggtctg acttcaatac
    14101 gttttcgctc gttttttatc gccaccgggc ttggtcaatt accggcgact attgtttatt
    14161 cctgggcggg cagcatgtta acaggcggta ctttctggtt tgtcaccgga ctgtttattc
    14221 tgtttgccct gaccgtggtg atttttatgg cgaagaaaat atggcttgag cgccagaaga
    14281 ggaatgactg atgggtttac cgccgcttag caaaattcct ttcattttac gtccgcaggc
    14341 gtggctgcat cgtcgccatt acggcgaggt gctaagcccg attcgctggt gggggcggat
    14401 cccgtttatc ttttatttgg tgtcaatgtt tgttgggtgg ctggagcgca aacgctcacc
    14461 gctcgatccg gtagtgcgat cgcttgtcag cgcgcgcatt gcgcaaatgt gcctgtgtga
    14521 gttttgcgtg gatatcacca gtatgaaagt cgccgagcgt accggcagca acgataaact
    14581 gctggcagtg gctgactggc ggcaaagccc gctctttagc gatgaagaac ggctggcgct
    14641 ggagtacgcc gaagccgcaa gcgtcacacc gccaacagtc gatgatgccc tgcgtacccg
    14701 actggctgcg cattttgacg ctcaggcgct caccgaactg acggcattga tcggcctgca
    14761 aaacctgtcg gctcgtttta attctgccat ggacattccc gctcaggggc tgtgccgtat
    14821 tccggaaaaa cgttcttaag gagggaagat gcgccattgt gggtggttgc tgggattgtt
    14881 atcgctgttt tctttggcaa cacatgccag tgactggcag gaaattaaaa atgaggccaa
    14941 agggcaaacc gtctggttta acgcctgggg cggcgatacc gcaattaacc gctatctcga
    15001 ctgggttagc ggcgagatga aaacccatta cgctataaac ctgaagattg ttcgtctcgc
    15061 ggatgccgca gacgcggtga agcgcattca gaccgaagct gctgccggac gtaaaacggg
    15121 cggctcggtg gatctgctct gggtgaacgg cgaaaacttc cgcaccttaa aagaggccaa
    15181 tttattacaa acgggctggg cggagactct gcccaactgg cgctatgtcg acacacagct
    15241 gccggtgcgg gaagattttt cagtgcctac agaaggggct gaatcgccct ggggcggcgc
    15301 acaactgacg tttatcgccc gccgcgatgt tacgccacag ccaccacaaa cgccgcaagc
    15361 cttactggag tttgctaaag ccaatcccgg cacggttacc tatccgcgcc caccggactt
    15421 taccggcacg gcgtttcttg aacagttgct gattatgctg acgcccgatc ccgccgcgtt
    15481 aaaagttgcc ccggataaag ccactttcgc ccgcgtcact gcgcccttgt ggcaatatct
    15541 tgacgcgcta catccctatt tatggcgcaa aggaaaggat ttcccgcctt cgcccgcgcg
    15601 gatggatgct ctgctgaaag ccggcacatt gcgcctgtcg ctgaccttta accctgctca
    15661 tgcccagcaa aaaatcgcca gcggcgattt gcctgcaagc agttacagtt ttggttttaa
    15721 ccaggggatg attggcaacg tgcatttcgt caccattcct gctaacgcaa atgccagtgc
    15781 tgcggcgaag gtggttgcca acttcctgct ctcacccaat gcgcaactgc gtaaagcgga
    15841 tcccgctgtc tggggcgatc cttctgttct cgatccgcaa aaactgcctg atgggcagcg
    15901 cgaaacattg caatcaagaa tgccgcaaga tctgccgccg gtactggctg aaccgcacgc
    15961 aggttgggta aatgcgctgg aacaagaatg gctacaccgt tacggtacgc attaatcttt
    16021 ttgctgtggg cgatggtggc ggtgatttat gcaccactga tcccggcagc tctcacgctg
    16081 atatcgcctg ctttgtcgtt gacacactgg caggcgttat ttgccgatcc acagttaccg
    16141 caggcattac tggcaacgct ggtgtcgaca accatcgcgg cggtcggggc gttgttgatt
    16201 gccctgctgg tgattgtggc gctgtggcct tggccgaaat ggcagcgtat gtgtgcccgt
    16261 ctgccgtggc tgctcgccat tccccatgtg gcttttgcca ccagcgccct tctgctcttt
    16321 gctgagggag ggctgcttta tgactatttc ccgtatttca ctccgctaat ggacaaattg
    16381 ggtattgggc tgggcctcac ccttgcagtg aaagaaagcg catttctgct gtggatctta
    16441 gcggcagtat tgagcgaaaa acggctgttg cagcaggtca ttgtgctgga ttcgctgggc
    16501 tacagccgct ggcaatgcct gaactggctg ctcttgccct ccgtcgcacc tgcactggca
    16561 atggcgatgt tggcgattgt tgcctggtcg ctttcggtcg tggatgtggc gattattctt
    16621 gggccaggta atccaccgac gctggcagta attagctggc agtggttaac tcagggcgac
    16681 gcggatcaac aaacgaaagg cgcactcgcc agtctgctgc tgatgctgtt actcgccgcc
    16741 tacgttttgc tgagctatct gctatggcgc agctggcggc gcactattcc ccgcgtagat
    16801 ggcattcgca agcctgccac acctttatta ccaggcacta cgctggcgag ttttttaccc
    16861 ttaaccggtg tgctgtgtgt ggttctgctg gcgatcctcg cggatcagtc gacgatcaat
    16921 agtgaagcac tcatcaacag cctgacaatg gggctggtgg cgacattcat cgctttgctc
    16981 ctgttactgc tgtggctgga atgggggcca cagcgtcgcc agttgtggct ctggttgccc
    17041 attctattgc ccgctctgcc actggtggcg ggccagtaca cgctggcgct atggctgaac
    17101 ctggatggaa gctggacggc ggtggtctgg gggcatctgc tgtgggtaat gccgtggatg
    17161 ctgtttatcc tgcaacccgc ctggcagcgc attgattcac ggttaatttt gattgcgcaa
    17221 acactgggct ggtcgcgggc caaaatcttc ttttacgtga aatgcccact catgttgcgc
    17281 cctgtgctga ttgccttcgc ggtgggattt tcagtcagta ttgcgcagta tatgccgacg
    17341 ctgtggctgg gcgcggggcg ttttccgacg ctcaccactg aggcggtggc attaagcagc
    17401 ggcggcagca acggtattct cgccgcccag gctttatggc aactgttgtt accgcttatt
    17461 atttttgccc tgaccgcgtt agtcgcaaaa tgggtaggtt atgtcagaca aggactccgc
    17521 taatgctctg cgtgaaaaat gtttcgctac gtttaccaga aagccgcttg ctgacaaacg
    17581 ttaactttac ggtggataaa ggtgacattg tcacgttaat ggggccgtct ggctgtggaa
    17641 aatccactct gttttcatgg atgattggtg cactggctgg acagttttct tgtacaggtg
    17701 agctatggtt caatgagcaa cggattgaca tgctacccac cgcacagcgt cagattggca
    17761 ttcttttcca ggatgcactg ttatttgacc agttcagcgt cgggcaaaat ttactgctgg
    17821 cgctaccgtc gacgctcaag gggactgcca ggcgcaatgc cgtgaaagac gcgcttgatc
    17881 gagcgggact ggctgaaact tatcaccagg accctgcgac attatcaggc ggtcagcgag
    17941 cgcgggtggc cctgctgcgc gcccttctcg ctcagccaaa agcgttactc ctggatgagc
    18001 cattcagccg tcttgatgtg gctctgcgcg ataattttcg ccagtgggtg ttcagcgaag
    18061 ttcgcgaact ggcgatcccc gtcgttcagg taacgcacga tctccaggat gttcctgctg
    18121 atagttctgt tctggatatg gcgcagtggt cagaaaatta caacaaactg cgataacgca
    18181 aagtttttct caatgcgtca gttcagaatg gcgcactcaa aactacaatg tcgggatttt
    18241 cgatgaaacg tgtttctcaa atgaccgcgc tggcaatggc tttagggctg gcttgcgctt
    18301 cttcgtgggc cgctgaactg gcgaagcctc ttacacttga ccagcttcaa caacaaaatg
    18361 gcaaagcgat agatactcgc cccagcgcgt tttataacgg ctggccacaa accttaaatg
    18421 gcccttctgg tcatgaacct gccgccttaa acctctctgc cagctggctc gacaaaatga
    18481 gcaccgaaca gctcaacgcg tggatcaagc aacataacct gaaagccgat gccccggtgg
    18541 cgctgtacgg taatgacaaa gatgtcgacg ccgtcaaaac gcgactgcaa aaagcaggtt
    18601 ttacgcatat ctccattctg agtgacgcgc taagcgaacc ttcccgtcta caaaaactgc
    18661 cgcactttga gcagctggtt tatccgcaat ggctgcacga cctgcaacaa ggtaaagagg
    18721 ttacggcgaa acctgccggt gactggaaag tcattgaagc ggcctggggc gctcctaagc
    18781 tttaccttat cagccatatt cccggtgctg actacatcga taccaacgaa gtggaaagcg
    18841 aaccgctgtg gaacaaagtt tctgatgaac aactaaaagc gatgctggca aaacacggca
    18901 ttcgccatga caccacggtc attctgtacg ggcgtgacgt atacgctgga gcgcgtgtgg
    18961 cgcaaattat gctctacgca ggcgtgaaag atgtgcgtct gctggatggc ggctggcaaa
    19021 cctggtccga cgcgggactg cctgttgagc gaggaacgcc accgaaagtg aaagcggaac
    19081 cggatttcgg cgtgaagatc ccggcacaac cgcagttgat gctcgatatg gaacaggccc
    19141 gtggactgct gcatcgccag gatgcatcgc tggtgagcat tcgttcgtgg ccagaattta
    19201 tcggtacgac cagcggttac agctatatta aaccaaaagg tgaaatagcc ggagcacgtt
    19261 ggggacacgc tggtagcgac tcgacgcata tggaagattt tcataacccg gatggcacca
    19321 tgcgcagcgc cgatgatatt accgctatgt ggaaagcgtg gaatatcaaa ccagagcagc
    19381 aagtttcatt ctactgcggc accggctggc gcgcgtccga aacctttatg tacgcacgcg
    19441 ccatgggttg gaagaatgtt tccgtgtatg acggcggctg gtacgaatgg agcagcgatc
    19501 caaaaaatcc ggtagcaacc ggtgaacgcg gcccggacag tagcaaataa cattgcaatt
    19561 actgacgctg gtgcgacttc agcgtcagat acccactcca cacccgcgta aaggtcgtca
    19621 tccagcacaa cgcaccgaag atccacgcaa accacgcaaa ccatgcaggg aataaacatc
    19681 ccagcacaaa cagtaagatc gtttctgtgc cttcggttaa cccgcccaga taataaaacg
    19741 atttatgcgc ataaccggga ttatcaatct gatgtttcgc cgccagcgca gcaaaagcga
    19801 gaaaactgct gcctgtgccg ataaacgcaa acagcagcca gccgcctgcc agggcgtttt
    19861 gctgcggtgc ggcgaggata aacccaaaag gtaccagcgc gtaaaaaaga aaatcgagag
    19921 aaatatcaag aaagccgccc gcgtcggtaa gccctctgcg ccgcgccagc gcaccgtcca
    19981 gaccatcaag cagcctgttc aacaaaatga cgacaagcgc cgccagatac cagcccagcg
    20041 ccagaaaagg cagtgccagc acgccgatgg caaatcccac taatgttaaa ccatctggcg
    20101 taatgcccgg tttatcaaga acccgcacgc actgatgcaa caacggttta atccggggat
    20161 gaagatggcg gtctagcaca ggcactcctt aaatataaag cctttctgat tgagcaacag
    20221 tgcggatatt atggcatttt tcgcttatct gcccgtgtgt aatttatgaa aatgattgaa
    20281 gttgttgccg ccatcattga acgtgatggc aaaattttac tcgcgcaacg ccccgcccag
    20341 agcgatcagg cgggattatg ggagtttgcc ggtggtaaag tcgagccgga tgaaagccag
    20401 cggcaggcgc tggtgcgtga gttacgcgaa gaactgggca tcgaagcaac tgtgggtgag
    20461 tatgttacca gccatcagcg agaagtttcg ggccggatta tccatcttca tgcctggcac
    20521 gtacccgact tccacgggac gttacaggca catgaacatc aggcgctggt ctggtgctca
    20581 cctgaagagg cgctgcaata tccgctggcc cctgctgaca ttccattatt agaggcgttt
    20641 atggctttac gcgccgccag accagcggat tagtgctaag ggttttgtca tcccgctggc
    20701 attgcagcag tattccctcg gctttaatta ccgcgccttc agaataattt ttatcctgat
    20761 aaacgcagca ctgagtacag ggctgcgctg actgcccgcc tgaactgaac acttctggcg
    20821 gtacgtttac ctccacgtcc ggacgataat gcgggttagc cagtgcaatt aatggaaatg
    20881 ctaatactac ggcgaacaat gctcgactca cagggaactc cttaacgtta ttgtctctgc
    20941 tactgataac ggtagccggg tggcaaaact ttagcgtctg agttatcgca tttggttatg
    21001 agattactct cgttattaat ttgctttcct gggtcatttt tttcttgctt accgtcacat
    21061 tcttgatggt atagtcgaaa actgcaaaag cacatcacat aaacaacata agcacaatcg
    21121 tattaatata taagggtttt atatctatgg atcagacata ttctctggag tcattcctca
    21181 accatgtcca aaagcgcgac ccgaatcaaa ccgagttcgc gcaagccgtt cgtgaagtaa
    21241 tgaccacact ctggcctttt cttgaacaaa atccaaaata tcgccagatg tcattactgg
    21301 agcgtctggt tgaaccggag cgcgtgatcc agtttcgcgt ggtatgggtt gatgatcgca
    21361 atcaggtaca ggtcaaccgt gcatggcgtg tgcagttcag ctcagccatc ggcccgtaca
    21421 aaggcggtat gcgcttccat ccgtcagtta acctctccat tctcaaattc ctcggcttcg
    21481 aacaaacctt caaaaatgcc ctgactaccc tgccgatggg cggtggtaaa ggcggtagcg
    21541 attttgaccc gaaaggtaaa agcgaaggcg aagtgatgcg tttctgccag gcgctgatga
    21601 ccgagcttta tcgtcacctg ggcgcggata ccgacgttcc ggcaggtgat atcggcgttg
    21661 gtggtcgtga agtcggcttt atggcgggga tgatgaaaaa gctctccaac aataccgcct
    21721 gcgtcttcac cggtaagggc ctttcatttg gcggtagtct tattcgcccg gaagctaccg
    21781 gctacggtct ggtttatttc acagaagcaa tgttaaaacg ccacggtatg ggttttgaag
    21841 ggatgcgcgt ttccgtttct ggctccggca acgtcgccca gtacgctatc gaaaaagcga
    21901 tggaatttgg tgctcgtgtg atcactgcgt cagactccag cggcactgta gttgatgaaa
    21961 gcggattcac gaaagagaaa ctggcacgtc ttatcgaaat caaagccagc cgcgatggtc
    22021 gagtggcaga ttacgccaaa gaatttggtc tggtctatct cgaaggccaa cagccgtggt
    22081 ctgtaccggt tgatatcgcc ctgccttgcg ccacccagaa cgaactggat gttgacgccg
    22141 cgcatcagct tatcgccaat ggcgttaaag ccgtcgccga aggggcaaat atgccgacca
    22201 ccatcgaagc gactgaactg ttccagcagg caggcgtact gtttgcaccg ggtaaagcgg
    22261 ctaatgctgg cggtgtagca acgtcaggcc ttgaaatggc gcaaaacgct gcgcgcctgg
    22321 gctggaaagc cgagaaagtt gacgcacgtt tgcatcacat catgctggat atccaccatg
    22381 cctgtgttga gcatggtggt gaaggtgagc aaaccaacta cgtgcagggc gcgaacattg
    22441 ccggttttgt gaaggttgcc gatgcgatgc tggcgcaggg tgtgatttaa gttgtaaatg
    22501 cctgatggcg ctacgcttat caggcctaca aatgggcaca attcattgca gttacgctct
    22561 aatgtaggcc gggcaagcgc agcgcccccg gcaaaatttc aggcgtttat gagtatttaa
    22621 cggatgatgc tccccaggga acatttctta tgggccaacg gcatttctta ctgtagtgct
    22681 cccaaaactg cttgtcgtaa cgataacacg cttcaagttc agcatccgtt aactttctgc
    22741 gatagcagca gatatgccag taaagaaatc ccattagact atttttttga taatcttctt
    22801 cgctttcgaa caactcgtgc gcctttcgag aagcaagcat tatataatgc caggccagtt
    22861 cttcttcaat tgtcccgttt tgaaaagctg tgcttgatat cgagatcatc catgataatt
    22921 ccgccgccca tattagcttc gccgaggatt taccggagcg attattagcg caatcagaga
    22981 tatagtctga gggaaaaaca gcaaatttat tcaacaaggc gataacctgc tctggggctt
    23041 cctccatgtt tgctttaaag gtattggctc catggtcgcc agaaagaaaa tgctccatta
    23101 aggcacaata actttcgcta tcttcgatac cccattgatc ctctaaagac tcgcgtcttt
    23161 tacttatgat atcgatcgag tcaaaaggaa gcacatgata ttggaaggta tctttgccag
    23221 gttcaggctt tcgcggccag aactccagcg tttcagacca ttgcttatga tagaatcgat
    23281 aaggtgcgat caattgtagc gcctgtaact tctcgatact gagcggctca atacctttag
    23341 cctgataata atgcagttgt tcttttttta ctttaaaacc ggcccgaaca ataagcccca
    23401 tcataattaa tagataaaga aaagagcatc c
//
LOCUS       DAZFFI010000070        22901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFFI010000070 DAZFFI010000000
VERSION     DAZFFI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.070"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..764
                     /gene="yneK"
                     /locus_tag="ACHJ12_003187"
                     /pseudo
     CDS             1..764
                     /gene="yneK"
                     /locus_tag="ACHJ12_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            914..2104
                     /gene="ydeA"
                     /locus_tag="ACHJ12_003188"
     CDS             914..2104
                     /gene="ydeA"
                     /locus_tag="ACHJ12_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4990938.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2129..2794)
                     /gene="marC"
                     /locus_tag="ACHJ12_003189"
     CDS             complement(2129..2794)
                     /gene="marC"
                     /locus_tag="ACHJ12_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4990939.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3006..3440
                     /gene="marR"
                     /locus_tag="ACHJ12_003190"
     CDS             3006..3440
                     /gene="marR"
                     /locus_tag="ACHJ12_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4990940.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3460..3843
                     /gene="marA"
                     /locus_tag="ACHJ12_003191"
     CDS             3460..3843
                     /gene="marA"
                     /locus_tag="ACHJ12_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4990941.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3875..4093
                     /gene="marB"
                     /locus_tag="ACHJ12_003192"
     CDS             3875..4093
                     /gene="marB"
                     /locus_tag="ACHJ12_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4990942.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4150..5589)
                     /locus_tag="ACHJ12_003193"
     CDS             complement(4150..5589)
                     /locus_tag="ACHJ12_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4990943.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5614..7287)
                     /locus_tag="ACHJ12_003194"
     CDS             complement(5614..7287)
                     /locus_tag="ACHJ12_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4990944.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7343..7654)
                     /locus_tag="ACHJ12_003195"
     CDS             complement(7343..7654)
                     /locus_tag="ACHJ12_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO4990945.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7682..9004)
                     /locus_tag="ACHJ12_003196"
     CDS             complement(7682..9004)
                     /locus_tag="ACHJ12_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4990946.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(9119..9430)
                     /locus_tag="ACHJ12_003197"
     CDS             complement(9119..9430)
                     /locus_tag="ACHJ12_003197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4990947.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            9629..10327
                     /locus_tag="ACHJ12_003198"
     CDS             9629..10327
                     /locus_tag="ACHJ12_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4990948.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(10372..11271)
                     /gene="eamA"
                     /locus_tag="ACHJ12_003199"
     CDS             complement(10372..11271)
                     /gene="eamA"
                     /locus_tag="ACHJ12_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4990949.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            11466..12653
                     /gene="ydeE"
                     /locus_tag="ACHJ12_003200"
     CDS             11466..12653
                     /gene="ydeE"
                     /locus_tag="ACHJ12_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4990950.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTISVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            12780..12875
                     /gene="mgtS"
                     /locus_tag="ACHJ12_003201"
     CDS             12780..12875
                     /gene="mgtS"
                     /locus_tag="ACHJ12_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4990951.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12875..12979
                     /gene="mgtT"
                     /locus_tag="ACHJ12_003202"
     CDS             12875..12979
                     /gene="mgtT"
                     /locus_tag="ACHJ12_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4990952.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(13094..13984)
                     /gene="dgcZ"
                     /locus_tag="ACHJ12_003203"
     CDS             complement(13094..13984)
                     /gene="dgcZ"
                     /locus_tag="ACHJ12_003203"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4990953.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(14239..14631)
                     /gene="ydeI"
                     /locus_tag="ACHJ12_003204"
     CDS             complement(14239..14631)
                     /gene="ydeI"
                     /locus_tag="ACHJ12_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4990954.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPALVRVTHLQK"
     gene            14907..15425
                     /gene="ydeJ"
                     /locus_tag="ACHJ12_003205"
     CDS             14907..15425
                     /gene="ydeJ"
                     /locus_tag="ACHJ12_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4990955.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(15470..17515)
                     /gene="dcp"
                     /locus_tag="ACHJ12_003206"
     CDS             complement(15470..17515)
                     /gene="dcp"
                     /locus_tag="ACHJ12_003206"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4990956.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            17652..18398
                     /gene="ydfG"
                     /locus_tag="ACHJ12_003207"
     CDS             17652..18398
                     /gene="ydfG"
                     /locus_tag="ACHJ12_003207"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4990957.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            18487..19173
                     /gene="rspR"
                     /locus_tag="ACHJ12_003208"
                     /gene_synonym="ydfH"
     CDS             18487..19173
                     /gene="rspR"
                     /locus_tag="ACHJ12_003208"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4990958.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            19351..19554
                     /gene="ydfZ"
                     /locus_tag="ACHJ12_003209"
     CDS             19351..19554
                     /gene="ydfZ"
                     /locus_tag="ACHJ12_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4990959.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(19590..21050)
                     /gene="ydfI"
                     /locus_tag="ACHJ12_003210"
     CDS             complement(19590..21050)
                     /gene="ydfI"
                     /locus_tag="ACHJ12_003210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4990960.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(21139..22422)
                     /gene="ydfJ"
                     /locus_tag="ACHJ12_003211"
                     /pseudo
     CDS             complement(21139..22422)
                     /gene="ydfJ"
                     /locus_tag="ACHJ12_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            complement(22735..>22901)
                     /locus_tag="ACHJ12_003212"
     CDS             complement(22735..>22901)
                     /locus_tag="ACHJ12_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4990961.1"
                     /translation="VTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEI
                     LAYRVGWAQE"
BASE COUNT         6188 a         5590 c         4990 g         6133 t
ORIGIN      
        1 tttcgccatg atggagaaat taatattttt gcgctcccaa cccaaagcct gtcactatgg
       61 gacgcgtatt tcagaccggc gtcgagcgtg tgctatttct gtttctgaat gattttattg
      121 aacaatttcc aatgttcaac cttggcgttc ccatcaaaag agcgcataca ccacatattg
      181 aacccctgcc tccggatcgc cataccgctg cagattactt acgccagttt gatttgcttg
      241 tcctgaattt tatctctcgc ggtaattttg tcatactccc ccgattatgg aataactctg
      301 aggttcacag atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa
      361 cagacggcga attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca
      421 aacatgtact acccgaagtc tgcatctgct ttttatccct tttagcagaa caagaatctc
      481 ctcattttca ggacttgttt ttatttttcg ccaatatgtt attgcactat caccaattta
      541 tgaatcccaa tgaaagtgat ttgaatgacg tgttgatgcc agcatcatta agtgatgata
      601 aaattatcaa acatatggcg cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc
      661 caaaagttac tcacgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta
      721 tacccgcaac cgccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga
      781 ctctctgacg cggaacagaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa
      841 tgcattgtat tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac
      901 ccgataatct gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt
      961 tacgctggca gtcgccgcct tcatcttcaa caccaccgaa tttgtccctg ttggcctgct
     1021 ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag gtcggcatta tgttgaccat
     1081 ttatgcatgg gtagtggcgc taatgtcatt gccttttatg ttaatgacca gccaggttga
     1141 acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt
     1201 tttatcgtgg agctttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc
     1261 gattttctgg tcgattacgg cgtctctggc gatccgtatg gctccggccg ggaagcgggc
     1321 acaggccttg agtttaattg ccaccggtac agcactggcg atggtcttag gtttacctct
     1381 cgggcgcatt gtgggccagt atttcggttg gcgaatgacc ttcttcgcga ttggtattgg
     1441 ggcgcttatc acccttttgt gcctgattaa gttacttccc ttactgccca gtgagcattc
     1501 cggttcattg aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt
     1561 gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt
     1621 gcaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg
     1681 tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt
     1741 ggtgagtacg gcgattgcgc tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa
     1801 cagtgaaata cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg
     1861 gcttggtatg caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc
     1921 gctattctcc ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt
     1981 gagtttgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc
     2041 gttaatttgg tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca
     2101 atagttgaaa ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc
     2161 cgttaataat aaactgcaca cccatacata ccagcaggaa tcccatcaga cgggagatcg
     2221 cttcaatgcc acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc
     2281 acaaaataac cgcaaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag
     2341 cgaaggttga actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg
     2401 gaccagcggt acttggcatt gctagcggca caaaggcaat attggcactg ggttcatctt
     2461 ccagctcttc cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga
     2521 gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg
     2581 aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa
     2641 atacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg
     2701 ccaggccaag aaataacgcg acagttgtta atgggttagc taacggcagt aacaccacca
     2761 gccccaggcc aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg
     2821 gttatcagcg aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt
     2881 gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattt
     2941 atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa
     3001 ctaatgtgaa aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc
     3061 atatggttaa tcagaagaaa gatcgtctgc ttaacgagta tctgtctccg ctggatatta
     3121 ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg
     3181 aactgaaaaa agtgttgtcg gtcgacctgg gagcactcac ccgtatgctg gatcgcctgg
     3241 tctgtaaagg ctgggttgaa aggttgccga acccgaatga taagcgcggc gtactggtaa
     3301 aacttaccac cagcggcgcg gcaatatgcg aacaatgcca tcaattagtt ggccaggacc
     3361 tgcatcaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc
     3421 ttaagaaagt cctgccgtaa acaaaaaaga ggtatgacga tgtccagacg caatactgac
     3481 gctattacca ttcatagcat tttggactgg atcgaggaca acctggaatc gccactgtca
     3541 ctggagaaag tgtcagagcg ttcaggttac tctaaatggc acctgcaacg gatgtttaaa
     3601 aaagaaaccg gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg
     3661 caaaagctga aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag
     3721 tcgcaacaaa ctctgacccg aaccttcaaa aattactttg atgttccgcc gcataaatac
     3781 cggatgacca atatgcaggg cgaatcgcgc tttttacatc cattaaatca ttacaacagc
     3841 tagttgataa cgtgacaacg tcactgaggc aatcatgaaa ccactttcat ccgcaatagc
     3901 agctgcgctt attctctttt ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt
     3961 tacttcttgt gccaatgtcg tggttgttcc cccatcgcag gaacaaccac cgtttgattt
     4021 aaatcacatg ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca
     4081 acacgctatg tagttattgt caggctctga tcacagagcc tgacatctcc gtaatatctt
     4141 cctcaagtct caaatatttt cgccattgtt ggcgatgaga tcccgatacc agtaaaacga
     4201 cgctttacgt atccgttcca gcgttccctt cccttcgttg tctttatcga cataaatcat
     4261 tccgtagcgc tttttcattt cccctgttcc ggcagaaacc aggtcaatgc agccccacgg
     4321 ggtgtagcca attaagtcaa caccatcttc aactacggct tttttcattt cccgaatatg
     4381 ggaagcaaag taatcaatgc gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc
     4441 aaccgcacca aatccatttt cgacaataaa cagcggcaac tggaaatgat cccagaacca
     4501 gttgagtgaa taacgtagcc ctgccggatc aatttgccat ccccattcgg atgtatcgat
     4561 ataagggttg ctgaccaaat ctcgtggttc aacataatcc agttgcgggt tatcgggcga
     4621 aaattgtgtc gtaaaagaca tgtagtagct aaagccgata aagtcgacac aaccactggc
     4681 aagaatcgcg ttatcttctg gtgtgatatc gaggttgaat cctttcctgg caaagtaatt
     4741 cagcatatgt tgcggataat atccacgagc atgaacatca gtaaaccagt aacgacgatg
     4801 cattgctttc gtggccatca tcatatcgtt gggtgcacac gtcagaggat agatgggaca
     4861 catggcgatc atacagccaa tgttaaattc aggattgatc gattttccag tctgtaccgc
     4921 cagggcactg gcaactaact cgtaatgtac cgcctggtac attatttgtt cgcgctcatt
     4981 gatatcttcc tctggcgaat acaagatacc ggaattagta aatggacaca ggctttcgct
     5041 gaaattcacc tgattattga tttcgttaaa cgtcatccag tactttactt tttctttata
     5101 gcgcgtgaag accgttgatg cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca
     5161 gccaccatat tttgtcacca gatgataagg catctcaaaa tgtgaaagcg tcaccacagg
     5221 ttccattccc tgcttaaggc attcatcgaa cagatcatca taaaattgta aaccctcttc
     5281 attcggctcc tgttcgtcac cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa
     5341 gcatttgaat cccatctcgg caaataactg aatatctgtt ttatagcgat gataaaaatc
     5401 aattgcttca tgattgggat aattaagccc gtcgataacg ccttctgtca cttcacgcgg
     5461 caccccgtga gcgccagcag tcatcacatc agcgatgctg atgccttttc ctccttcatt
     5521 ccagccacct tccaactgat gcgcggctac cgcaccgccc cataaaaaac cttttttaaa
     5581 tcctgacata cacaactcct taactaaaat agattaccac cagacttccg cctggatgcc
     5641 ggccataaac tcgtctttgc tgttctcatt aaacttaaaa ttcgataatt cgttatccag
     5701 aatattgata tacgttccat agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc
     5761 cactttcaat gcatgccaga gtgtcgtttt ataagccgat tcatttagtg ttacaccctg
     5821 ctgagttttg ttctgttgct taaaccagcc taattcaagc cccgtctgat tccatgtatt
     5881 ccagatccag gccggtctta ttacagtgcg aatactgtca aaatcactat gagcaccact
     5941 ttcataacta taaacatctt cgccgtgaga atagacaagc gcgttagcca taataatatt
     6001 gtcagtaaga tacatctcgc cctgagagat taaacgccag gcgatcccat tggtatgatc
     6061 accatagtaa tagcgaccat gcgccatcgt attactggca tctgagaaac tggcaaaact
     6121 gctggcatag gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag
     6181 gttttgccgt aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgttgtc
     6241 attttcgtta tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga
     6301 taatgtcgca tcatcccata acgggatatt gcgataacga acatccacag aattggtatt
     6361 catctggctg gtacgcgaaa aatcacgaga gtaaacatcg acatcatcac ggcttaagga
     6421 catatcaaac agccctacac caagtgccca gttttcaatc cccacacccg cggcgacatc
     6481 cgtggttaag gttttccagt ccagcatctg gatctcatat tgcgggagtt tatgtttgcc
     6541 gacccagaag tctgcctctg gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc
     6601 gctgaactgc atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctc
     6661 acccacgttg ccatcatagg taacgaccgc atttgccgtt ttaccgtcct gattataaac
     6721 acgctgattt aaggtcaggt caaaccaacc gctcatctca ttaccaaaac gtcccagaga
     6781 acccgctgca taagtttgtg gtgagccgtg attaccagta ccccagcctg agcggaagta
     6841 cccctgatag ctgaacccaa tatcatcctt tatatattta ctgatatctt tcagggtcac
     6901 gctttcgata gtggttttgt cgcctttact ttcaataacg gcaacttgct gcgatccatt
     6961 tatagtatgc gctgcggcag acgagttctg ttcaccgttt tcatttttga tgttatcagc
     7021 gacgggagat gtcgctgata tttcagcaag cgaggctgat ttatatttgt tttccgtgat
     7081 gcttttttgt aaggtcgaaa gtcgggattt atatactccc agttcattct gtgttgcttt
     7141 cagctcttgt ttattttgcg acaattcatt ttcaagcaat tcaaggcgct gctctatggt
     7201 taattttgtt gccatcgcgt tcaatggaaa caatgctgta ataatagaaa aagacaaaag
     7261 gctgacgtta agcgtcttta tattcatgac ttatccttaa gagtataata atttcctcca
     7321 gctatcgcac tggaggaaaa aatcaggcat ggagttcttt tcttaatgaa ataatttccc
     7381 tggctaaatc ctggcataat attgcattca tcaaatgatc ctgtgcatga atcagaatta
     7441 aattaacttt tattttgcca cagccttcat cctgactaat taatgcggtc tgaatcttat
     7501 gtgcctgaag acaggcagat tcagaactgg ccattagctg agttgcgccc tgccagtctt
     7561 tttgcccggc acaacgtatg gcttccatcg cgtcagaacg tgcctgcccc gcgttgacca
     7621 gtaactccat aacaagttct tcatctgcaa acatcacaat ctccttcctg ggtaaatgga
     7681 attatccgtt agttgtcatg ttattgactt tgttggcggc aataacaaaa ggtaaataga
     7741 tcattgtcgc cacacctaaa cataaaaaac caaccagtaa agccagccag ttccccccgg
     7801 ttccgaggaa agcaattaaa ggtccgggcg tagtccaggg cacggcataa gcaaccggtg
     7861 gaataatttc catcgaaaca aagaagtaac caatggcaca gttaacaatg ggaaccataa
     7921 taaacggcac catcatgatg gggttaagta caataggaag gccaaacatt atcggttcat
     7981 tgatattgaa aataccgggg ataaatgaca tttttgccag atcacggtat tccgcacgct
     8041 tagaagcgat aaaaatagcc aacaataacc ccaacgtcat acctgaaccg ccgcagttgg
     8101 cgaatgcatc attaatagaa gtccaggtaa tcggatatgg cgcgccccag gtagtgccat
     8161 gttgagcggc ccaggagaga ttctccagat tagcctcaga aaaaacggtt tctcgaattg
     8221 ccgatgtggt attaggtccg tggatcccca gcacccacag gaaattaccc acagccccca
     8281 ggataattac cgcaaagata ttcgttccca tatgtttgag cggcgtctgg attaatgtgt
     8341 aaataagatc gtttagtcct gccggggata tcagtgtcag gcaataatta agtgcggaaa
     8401 agaacaccat gacaaaaaat attggcaata aaactttaaa tgaacgcgct accgcaggcg
     8461 gtacagctgc cggcatcgtt atggtgattt ttgggtttcg agctaagcga caaaatattt
     8521 cactggtgat caatgcaacg ataacagcaa caaacaaccc ctgcgcgccg agatatttgg
     8581 tcgtcaggaa tgcttgccca tctatcatct gatatggcgt ataaacaata aaaaatgctc
     8641 ctattgccgt taatccgcat aaaagatcat cttgctcata gctaatcgcc atattcctgg
     8701 cgaccaggaa agcaatcata attgacatga tatttacaga accattcatt accggagtaa
     8761 aaatagcttg tgctttttca aggttgggga aaatgctatt cagatgcagt aatccggcaa
     8821 taaaaccgtc tggcgataat atggcaaagt taattaatat aattaatgaa cttgccatga
     8881 taattggaaa cgtaaaaacg aatccatctc tcaccgctgc aacatgtttt tgtgagttta
     8941 acttgatagc aactggaaca agaaaacgtt ccattccacg ttcgaatgag gccattaatc
     9001 ccattttttt atacctgttg ttttggatat ttatacatcc caataaagca tgtatgccat
     9061 atccatttta tgatgtgtga aaagttagtt caggtaatag tttaataata ttagattctt
     9121 actcttgcag tagtttcatc gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt
     9181 ctttcatctc gataacggct acaggcattt tcccctgtgc aacggcttca atctctggtt
     9241 tctgaaagcg aacttgcggg ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc
     9301 ctttagcctc tgcaatcgcc aatgcggaaa tattaacttc aagtgaaata gcgttagcat
     9361 gatcaatcat acgtttaacc agcatactgg ttgacatgcc cgcagcgcaa actaacaata
     9421 tttttttcat attttccctt actggtatat aacagattac attgtttaaa tatcatatcc
     9481 cgcagtagct atacgtcctg gtacattgat cacagtaata cagtgataaa gttgttcaac
     9541 atcagggtga ttattaaacg actcggccat acgataaacc aatcaggact caactccttg
     9601 ttttcaatgg ctcttaaaag gtgcaaaagt gatttttcaa aagattgccc gcttacttaa
     9661 atccgaaatc aatggcaatt catggcatgt tggtgatttg ttgccgtcag aagcggaact
     9721 cgctgttcgt tataatgttt cgcgtaatac cctgcgtaag gcattatcct tgctggaagg
     9781 cgaaggaatt attcacagaa agcatggttc aggaacatac attcaaaaaa agaattttgt
     9841 tgcacacatt gatcacatga acagtttcag tgaaattgcg cataaaagcg gcaaagaggc
     9901 agggagtcag attatgaaat ttgaagtgca ggatgcttca cccactattg cgactgagct
     9961 gaatttagtg actggtgagc aggtttatta cataaagaga ctgcgattca ttgaggataa
    10021 tgcagcacaa ttagaagaaa cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc
    10081 acatatgcaa aaatccaaat tttcatatat tgagaatgaa tgcgggatca aaatcattgg
    10141 cacctttgaa actttctccc ctacttttcc taccccagaa atcgccagta ttttacggat
    10201 cagcccacgg gatcccatac ttaaaattca gacccaggct gtggacagta actctattcc
    10261 gctggattat tcgttacttt acagcaatat tttcgagttc caggtaaagt actttttccc
    10321 gcgataaaag cagacgcaca gagtgctgac atcacattct gtgcgtatgg tttagcctct
    10381 aacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10441 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10501 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10561 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10621 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10681 atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10741 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10801 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10861 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10921 caattgtttg ccatgcaatc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10981 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccgaagt taatggcaca
    11041 aaaaagaaaa gcaaactgcg caaaactgat ggttaatcca taccccagca gcaaattcag
    11101 tggtactttc ggtcgtgcga caaaaaagat agccggaaaa gcgaccagca taaagcgcaa
    11161 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    11221 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    11281 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11341 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11401 tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca ctgccggaca
    11461 gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg tcgttgttat
    11521 taaccatcgg acgcggcgct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11581 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt agcgtcgttt
    11641 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11701 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtaaagctgg
    11761 tggtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11821 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11881 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11941 gcatcaatct gcccttctgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    12001 aaatttgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    12061 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    12121 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    12181 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    12241 cgttgcaata ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag
    12301 caggcaccct atgtttcgtc atcggtctgg tcggttttat tttttccggc aacagcctgc
    12361 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12421 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12481 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12541 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12601 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    12661 cgaacacaat aaaaatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc
    12721 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    12781 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    12841 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    12901 ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc
    12961 gccagtaaac cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac
    13021 aggcattttc ctgcactgat aacggatcgt tgacacagta gcatcagttt tctcaatgaa
    13081 tgttaaacgg agcttaaact cggttaatca cattttgttc atcaataaac atgcagcgat
    13141 ttcttccggt ttgcttaccc tcatacattg cccggtctgc tcttccaatg accacatcca
    13201 gaggctcttc aggaaatgcg cgactgacac ctgctgtcac ggtaatgttg atatgccctt
    13261 cagaatgcgt gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg
    13321 cttcttcatc attagccgct ttgacaataa tgataaattc ttcgcccccg tagcgataaa
    13381 ccgtttcgta atcacgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat
    13441 cgccgattaa atgcccgtag gtatcattaa ccaatttaaa tcggtcaata tccaacaaca
    13501 ttaaataaag attcagaggc tcagcgttgc gtaactgatg atcaaaggat tcatcaagaa
    13561 cccgacgccc cggcaatccc gtcaaaacat ccatattgct acggatcgtc agcaaataaa
    13621 ttttgtaatc ggttaatgcc gcagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat
    13681 gcgcgtcctg ccagtgattt tcaacaatag ccagcattaa ttcccgacca cagttatgca
    13741 tatgttgatg ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc
    13801 ccagatgatc aatccaccga ccaaactggc acagtccata agaatggtta tccgttattt
    13861 ctggcttact ggcatctctc gcgaccacgc tgtgaaacat actcaccagc cactggtagt
    13921 gggcatcgat agccttattg agatttaaca agatggcatc aatttccgtt gttttcttga
    13981 tcattgccac tcctttttca cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa
    14041 attacgtcaa tattttcata gaaatccgaa gttatgagtc atctctgaga taacattgtg
    14101 atttaaaaca aaatcagcgg ataaaaaagt gtttaattct gtaaattacc tctgcattat
    14161 cgtaaataaa aggatgacaa atagcataac ccaataccct aatggcccag tagttcaggc
    14221 catcaggcta atttattttt atttctgcaa atgagtgacc cgaacgaggg ccggcgcgct
    14281 tttcttatcc agactgccgc taatgttgat catctggtcc ggctgaactt ctcgtccatc
    14341 aaagacggcc gcaggaataa cgacattaat ttcaccgctc ttatcgcgaa aaacgtaacg
    14401 gtcctctcct ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc catcgtgcat
    14461 ggtatttgcg aaatcaacgg tcattttttt tgcatcatcg gttccgcgat agccatcttc
    14521 tattgcatga ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt catctgccaa
    14581 cgcataaggc atgacaagaa aacttgctaa tacaatggcc tgaaatttca tactgactcc
    14641 ttaattgcgt ttggtttgac ttattaagtc tggttgctat ttttataatt gccaaataag
    14701 aatactgcca attgttataa ggcatttaaa atcaggcaac tagctgtcaa atattcagag
    14761 aatttaactc actaaagtta agaagatcaa atagtcttaa atatattttc agaataatcg
    14821 gatttatatg tttgaaaatt attatattgg acgagcatac agaaaaagca aatcaccttt
    14881 acatataaaa gcgtggacaa aaaacagtga acattaatag cgataaaatt gtacaacttg
    14941 cagataccga cactattgaa aacctgacat ccgcgttgag tcaaagactt atcgcggatc
    15001 aattacgctt aactaccgcc gaatcatgca ccggcggtaa gttggctagc gccctgtgtg
    15061 cagctgaaga tacacccaaa ttttacggtg caggctttgt tactttcacc gatcaggcaa
    15121 agatgaaaat cctcagcgta agccagcaat ctcttgaacg atattctgcg gtgagtgaga
    15181 aagtggcagc agaaatggca accggtgcca tagagcgtgc ggatgctgat gtcagtattg
    15241 ccattaccgg ctacggcgga ccggagggcg gtgaagatgg tacgccagcg ggtaccgtct
    15301 ggttcgcgtg gcatattaaa ggccagaact acactgcggt tatgcatttt gctggcgact
    15361 gcgaaacggt attagcttta gcggtgaggt ttgccctcgc ccagctgctg caattactgc
    15421 tataaccagg ctggcctggc gatatctcca ggccagccat tggtggtgtt tatatgttca
    15481 agccacgatg ttgcagcatc ggcataatct gaggagcctt accgcgccat tgtcgataca
    15541 ggcgttccag atcttcgcta ttacctctgg aaaggatcgc ctcgcgaaaa cgcagcccat
    15601 tttcacgcgt taatccgccc tgctcaacaa accactgata accatcatcg gccaacattt
    15661 gcgtccacag ataagcgtaa taacctgcag catatccgcc accaaaaata tgggcgaaat
    15721 aactgctgcg atagcgtggc ggtatagcag gaagatccat attttccgcc accagcgccc
    15781 gcaattcaaa atcatcgaca tcctgcattg cttcgttttc ttccaggcaa tgccagcgca
    15841 tatcgagaag tgcggcgcta agcagttcgc tcatctcata ccctttgttg aacaggctgg
    15901 cattacgcat tttctgttgc agttcgtcag gcattgctgc cccgctctga taatgccggg
    15961 cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc gttgatttgc gacggaaatt
    16021 cgacaaaatc acgcggcgtg ttggtgccgg aaagcgtggc ataacgctgg cgggcaaaaa
    16081 ggccgtgcag cgtatgacca aattcatgga ataaggttat tacatcatcc cagagtaaca
    16141 acgcaggctc accggcagcg ggtttctgat aattgcagac gttataaatt accggatgcg
    16201 tttcattaag cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg ctttttgaat
    16261 cacgggcgaa gaaatcaccg taaaataacg ccagtcccac gccattatga tcaaaaattt
    16321 cccacacacg aacgtcagga tggtagacag gaatatcaaa acgttcgaca aacttaatac
    16381 cgaagagctg attcgcggtc cagaatacac cttcattcaa caccgtgttt aattcaaaat
    16441 atggcttgag ctgcgcctca tcaagatcaa atttctcccg ccgtacctgt tcggcataaa
    16501 atgcccagtc ccacggctgc gcgctaaacc cgccctgctg cttatcaata accgcctgta
    16561 tggaggctaa ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc cgcataaagt
    16621 tgagcgctgc ttcaggtgtt tttgccatct gatcggcgat tttccatgcg gcataatgag
    16681 gaaaaccaag taatgtcgcc tgttgtgcac ggatctccac cagacgttga atgatagcgc
    16741 gggtatcatt gccatcattt ttttccgctc gcgtccagcc cgcaataaac agtttttcac
    16801 gcgtcgcacg atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc agcagcggaa
    16861 tcagccattt gttatccaga cctttctcgc gagccgcctc tgccgccagc gcaatctctt
    16921 gctcactcat tcctgccagc tgcgcgatat cgttcacaac cagaccgccg gatttatttg
    16981 ctgccagtaa ccgctggtta aactgactgg tcagtgtcgc agcttctgta ttcagtactt
    17041 ttaattttgc tttatcagct tgcgcaagtt tggctccggc aaggacaaaa cgttgatgaa
    17101 tcacctccac caggcggatg gattcactat caagccccag ggattcacgg cgctgccaga
    17161 cagcatctac ccgcgcgaat aattcaccgt tcagatagat atcattagcc agttccgcca
    17221 gttcagcgga aaactgttca tcaagacgct gtaattcatc attggtatgc gccgcagtca
    17281 tcgcaaaaaa gacgctggta acgcgggtaa gtaattctcc gctttgttcc agtgccagaa
    17341 tagtattgtt gaaatcaggc gtttgcgggt taagcgcgat ggcagcaatt tctgcccgct
    17401 tttgctgcat tccctcatcg aatgccgggc gatagtgatg attggcaatt tgatcaaaat
    17461 gaggagccag atacggcagt gtgctttgca caaggaaagg attcattgtt gtcattttct
    17521 tctcctgaac gcgaggtgtt ccatagcgta ggcttactga taacggagtg caatcttgca
    17581 aaccagtatt acccgctctt aagcatcacg tgctatgtta ttgacacaca aaagcgttga
    17641 ggaacagtga gatgatcgtt ttagtaactg gagcaacggc aggttttggt gaatgcatta
    17701 ctcgtcgttt tattcaacaa gggcataaag ttatcgccac tggccgtcgc caggagcggt
    17761 tgcaggagtt aaaagacgaa ctgggagata atctgtatat cgcccaactg gacgttcgca
    17821 accgcgccgc tattgaagag atgctggcat cgctccctgc cgagtggtgc aatattgata
    17881 tcctggtaaa taatgccggc ctggcgttgg gcatggagcc tgcgcataaa gccagcgttg
    17941 aagactggga aacgatgatt gataccaaca acaaaggcct ggtatatatg acgcgcgccg
    18001 tcttaccggg tatggttgaa cgtaatcatg gtcatattat taacattggc tcaacggcag
    18061 gtagctggcc ttatgccggt ggtaacgttt acggtgcgac gaaagcgttt gttcgtcagt
    18121 ttagcctgaa tctgcgtacg gacctgcatg gtacggcggt gcgcgtcacc gacatcgaac
    18181 cgggtctggt gggtggcacc gagttttcca atgtccgctt taaaggcgat gacggtaaag
    18241 cggaaaaaac ctatcaaaat accgttgcat tgacgccaga agatgtcagc gaagccgtct
    18301 ggtgggtgtc aacgctgcct gctcacgtca atatcaatac cctggaaatg atgccggtta
    18361 cccaaagcta tgccggactg aatgtccacc gtcagtaatt tttatacccg gcgtaactgc
    18421 cgggttattg cttgtcacaa aaaagtggta gactcatgca gttaactcac tcacaagcaa
    18481 gaacgaatga ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca gcagatttat
    18541 cgtattcttc gtcgcgacat tgtccattgc ctgattgcac caggcacacc gttgtcggaa
    18601 aaagaagttt ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc ctttattaaa
    18661 ctggcggaaa acggcctgat tcaaattcgg ccgcagcgtg gcagctacgt caacaaaatt
    18721 tccatggccc aggtgcgaaa cggcagtttt atccgtcagg ccattgagtg cgcggtggcg
    18781 cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc aactggaaca aaatcttcac
    18841 cagcaacgca ttgccattga gcgcaagcaa ctggatgatt tttttgaact tgatgacaac
    18901 ttccatcaac tcctgacgca gattgccgac tgtcaactgg cgtgggatac cattgagaac
    18961 ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg accatgtttc tccaccagaa
    19021 atgctgttac gccagcatct tgatattttc tctgccctgc aaaaacgtga tggcgatgcg
    19081 gtagaacgtg caatgacgca acatttgcag gaaatcagcg aatccgtgcg ccagatccgc
    19141 caggaaaaca gcgactggtt tagcgaagag taattcattt actctcatcc catccgggat
    19201 gagaggcttt tccccccgcc ttacggctca tgcatgcatc aaaaaagatg tgagcttgat
    19261 caaaaacaaa aaatatttca ctcgacagga gtatttatat tgcgcccgtt acgtgggctt
    19321 cgactgtaaa tcagaaagga gaaaacacct atgacgacct acgatcgtaa ccgtaacgca
    19381 atcaccactg gcagccgtgt tatggttagc ggcaccggtc acactggcaa gatcctgtcg
    19441 attgatactg aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt agttgttgaa
    19501 ggttgtgaag agaaactggc acctctggac ctgattcgtc tcggcatgaa ctaagtgtgt
    19561 gaatgccgcc gatggcggca ttgctttttt tacttcacgg aatatttcgc cacggtcgct
    19621 ttcgcgccat gcgctaataa agacaagtac gcttccgtca cttttgcagt aaacaagcta
    19681 ttgtctggca aatcatcacc aaagattgcc ttaatcgcca gcaatgactg gacgcgcgct
    19741 ttcccttcgg cactactttg tacagccttc tgaataacag gtaacagtgg gtcactgatt
    19801 tctatcggat ttccctgttc atcaacacca ccgacataac gcatccaacc cgcgacgccc
    19861 agcgccagca gatcgaactt gctgtcatgc gccagatgcc agcgaacaga atccaacatc
    19921 cgctgtggca atttctggct accatccatc gcaatctgcc aggttcgatg acgtaacgcc
    19981 gggttgctat agcgtgcaat taatcggtta gcgtaatctt gcaaatcaac gccctgcact
    20041 ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg ccgcataacg ataatgttca
    20101 tcttccatgc agtcattaat gtgctgatat ccagcaagat aacccaaata cgccaggaat
    20161 gaatgactgc cgttgagcat gcgcaacttc atctcttcat aaggcagcac atcactaacc
    20221 agttcggctc ccgctttttc ccattctgga cgtccggcaa caaagttatc ttctattacc
    20281 cactggcgga aaggttcaca ggcaacgccc gcaggatcgc ccacaccggt aagttgttcg
    20341 attttcgcca gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat tgttgatggg
    20401 aaagtcacgt tatcttcgat ccattgtgcc agtttttcat caacggcttg cgcgtaggaa
    20461 gtgacaacgt cacgcataac atgaccgttt tctggcatgt tgtcacatga catgacggta
    20521 aatgcgggaa gtcctgccgc tttacggcga gccagcgcct caacaatcac tcctgttgct
    20581 gttttcggct ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc gagcattaac
    20641 tgtccggtcg ccggagagtg gaaataccct ttttcggtga ttgtcagaga gacaatcgcg
    20701 atttgcggtt cacacatcgc agccaacacg gtttctaagc catccatctg tacgtgcaag
    20761 gcttttttaa cgacgccaac gacgcgagcc gtccacgcat cggccgacat ttccgcaacg
    20821 gtataaagat tatcttgctg ctgtaaatcg gcaatttgct gttcgccgcc gattaagttg
    20881 acctcataat atccccagtc actgaaatgt tctgtagcaa gaatatcggc atacacgccc
    20941 tgatgcgcac ggtgaaatgc accaaagcct aaatgaacaa ttcttggggc caggttatta
    21001 cgatcataaa cagggagtgt cgcttttgct gataacaaat tatttcccat aacaattcct
    21061 taaatttaaa tatggcaagc tacatgtttt gttatatgaa taaaaatccc ctctccggta
    21121 agagaaggga ttgaggattt acagacttct ggaaggttgc acagctctta caacacgcgg
    21181 ttgatcttcc gcagcgtctt ccagcgcact taaatcacgg tctttcactt ctggcatttt
    21241 cagcgcagag attaaaccaa tcactgaata tgccatgatc ataatggcga tcggatacca
    21301 ggattccgtc atggtgcaga aaatacccgc caggatagga ccaaaaccgg aagcgataag
    21361 accaccaatt tctttagaaa tggccatccg ggtaaagcgg tttttacagc cgaacatttc
    21421 tgccatggta atgttttcca gagcaaataa tcccagcacc gcacagttat gaatcacaat
    21481 cagtgcaacc ataatggtgc tcggggcata gcttttatct acaatgatag aaagcattgg
    21541 ccatgccagc acaatcgcgg aggtattcat aataatatac gggatccggc gaccaatttt
    21601 atcggataac caaccaagga acggaatggt cataaagccg agaatcgaac taatcatcaa
    21661 tgcatctgtt ggaattgctt tgttaaacaa taacgtctgc actaaatagc ctgcaaggaa
    21721 agtctgaatt aacccggagt tacccgcctg accaaaacgc agccctgttg ccagccagaa
    21781 ggatttgctc tggaacatgc taccagcagg tgcaggtttt gctgtgggtt ggttgctgtc
    21841 attaaccttc tcaaagaccg ggctttcttt cagattcata cgtaaccaga tagcaaagac
    21901 catcacgaca acgctcgcca ggaacggtat acgccatccc cacgccagca gttcctcttt
    21961 actgagaatg aagaacataa aggcccagat tgccgttgcg ctcaaggttc cgcagttagt
    22021 tcccatagct acaaatgagg agataattcc gcgcttacct tttggtgcat attccgccag
    22081 catcgtaccg gcaccggaaa tttccgcacc tgcacccaac ccctgaataa tacgcagcgt
    22141 caccagcaag atgggggcaa aaacaccaat ctgtgcatag gtcggtaaca caccaattaa
    22201 ggtggtacag atccccatca tggtgatggt aataaagagc acttttttac gccctattct
    22261 gtcgcccatt ttgccgaaaa taaatgctcc gacaatacgc gccacataac ctgcaccgta
    22321 ggttcccatt gccaaaatta acgccattgc cgttgatgat tcaggaaaaa atatttcatg
    22381 aaacactaac gctgcgccga gcgaatataa ctggaaatcc ataattcata gatgttttct
    22441 aacattctgt ggtcttattt gcgttttcta agttttttca gataattaca tttttcaata
    22501 agaatcctga actcgatttc tctgtttatc tgaggacttc ttttgtccca tatatgcccc
    22561 aagatgcccc atgcaaatcc catgaacaaa aagcacgcaa aaatgagagc tccatcactt
    22621 ccggaattaa cttttccgtg gcactcttta tctgtgtagg taaggtctct tcacaataat
    22681 tacatagtca acgcagttca tgggggaggt gatggtcccc catctttttg tctgttattc
    22741 ctgtgcccag ccaaccctgt atgccagtat ctcatccgca ctggtcagct tttccagctc
    22801 cttcttcatg gtgcgctgcc ggacgtggat ttccatccct ttggtgaaca tcgcctgctc
    22861 tgccgcttca ctcagcgcta taagctcttc tgctgtcact t
//
LOCUS       DAZFFI010000071        22764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFFI010000071 DAZFFI010000000
VERSION     DAZFFI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.071"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(163..1812)
                     /gene="actP"
                     /locus_tag="ACHJ12_003213"
     CDS             complement(163..1812)
                     /gene="actP"
                     /locus_tag="ACHJ12_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4990962.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1809..2123)
                     /gene="yjcH"
                     /locus_tag="ACHJ12_003214"
     CDS             complement(1809..2123)
                     /gene="yjcH"
                     /locus_tag="ACHJ12_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4990963.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2324..4282)
                     /gene="acs"
                     /locus_tag="ACHJ12_003215"
     CDS             complement(2324..4282)
                     /gene="acs"
                     /locus_tag="ACHJ12_003215"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4990964.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4675..6111
                     /gene="nrfA"
                     /locus_tag="ACHJ12_003216"
     CDS             4675..6111
                     /gene="nrfA"
                     /locus_tag="ACHJ12_003216"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4990965.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6156..6722
                     /gene="nrfB"
                     /locus_tag="ACHJ12_003217"
     CDS             6156..6722
                     /gene="nrfB"
                     /locus_tag="ACHJ12_003217"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4990966.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6719..7390
                     /gene="nrfC"
                     /locus_tag="ACHJ12_003218"
     CDS             6719..7390
                     /gene="nrfC"
                     /locus_tag="ACHJ12_003218"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4990967.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7387..8343
                     /gene="nrfD"
                     /locus_tag="ACHJ12_003219"
     CDS             7387..8343
                     /gene="nrfD"
                     /locus_tag="ACHJ12_003219"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4990968.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8363..10081
                     /gene="nrfE"
                     /locus_tag="ACHJ12_003220"
     CDS             8363..10081
                     /gene="nrfE"
                     /locus_tag="ACHJ12_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4990969.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFMRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10074..10457
                     /gene="nrfF"
                     /locus_tag="ACHJ12_003221"
     CDS             10074..10457
                     /gene="nrfF"
                     /locus_tag="ACHJ12_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4990970.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10454..11050
                     /gene="nrfG"
                     /locus_tag="ACHJ12_003222"
     CDS             10454..11050
                     /gene="nrfG"
                     /locus_tag="ACHJ12_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4990971.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11392..12705
                     /gene="gltP"
                     /locus_tag="ACHJ12_003223"
     CDS             11392..12705
                     /gene="gltP"
                     /locus_tag="ACHJ12_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4990972.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12783..13472)
                     /gene="yjcO"
                     /locus_tag="ACHJ12_003224"
     CDS             complement(12783..13472)
                     /gene="yjcO"
                     /locus_tag="ACHJ12_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4990973.1"
                     /translation="MKKIIALMLFLTFFAHANDSELGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13566..15713)
                     /gene="fdhF"
                     /locus_tag="ACHJ12_003225"
     CDS             complement(13566..15713)
                     /gene="fdhF"
                     /locus_tag="ACHJ12_003225"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15294..15296),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO4990974.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15911..16312)
                     /gene="mdtP"
                     /locus_tag="ACHJ12_003226"
                     /pseudo
     CDS             complement(15911..16312)
                     /gene="mdtP"
                     /locus_tag="ACHJ12_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(16557..16638)
                     /locus_tag="ACHJ12_003227"
     tRNA            complement(16557..16638)
                     /locus_tag="ACHJ12_003227"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16598..16600),aa:Tyr,seq:gta)
     gene            complement(16662..16756)
                     /locus_tag="ACHJ12_003228"
     tRNA            complement(16662..16756)
                     /locus_tag="ACHJ12_003228"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(17045..17452)
                     /locus_tag="ACHJ12_003229"
     CDS             complement(17045..17452)
                     /locus_tag="ACHJ12_003229"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO4990975.1"
                     /translation="MNEQTHHVPKDIERLKFIDAIDNGDKPGDAWCDSLADIVSHSVA
                     ELTRNGMEEADAFRIAKRVVAAIAFYAGGRSLYLPTGRSLFAALREYEIYYRWDNGEK
                     LQTLRKEYAISETHIYDIIRRLRKKHANKITQD"
     gene            complement(17566..17838)
                     /locus_tag="ACHJ12_003230"
     CDS             complement(17566..17838)
                     /locus_tag="ACHJ12_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4990976.1"
                     /translation="MVHDRIAEELESKGFYRRTAARWAEVMMLVETDRERERVAMRRA
                     ECYGKAQRPPEPPEENYADLRKAVNRTYAEMGIGGRCKKIWRSYQK"
     gene            complement(17849..18259)
                     /locus_tag="ACHJ12_003231"
     CDS             complement(17849..18259)
                     /locus_tag="ACHJ12_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4990977.1"
                     /translation="MTAQIAAYGRLIDDPQTKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADQLAHHEKGNVISVSGVMQVSQWTGQNGETRQGYQVIADSVISA
                     KTAQPGGSRRRTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(18702..20099)
                     /locus_tag="ACHJ12_003232"
     CDS             complement(18702..20099)
                     /locus_tag="ACHJ12_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4990978.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQEGRLAGDN
                     VPPVWLGEQQLAELTDMRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVQDANYY
                     PDGMHSQKRENWREYLERERGEKQEEKGHQRKTKLPMSVGSAGYDTQLDYVVKGIIPA
                     SSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVKA
                     WEVVHGEQVKNLYLVNRPIFPAVPLDVDEMVIAARQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYRI
                     RREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPVE
                     QERIEEAGNKTENHTALWGCIRSRTQHGDKCTISLLRDDMKRLGYDVKNMRRWLAKLE
                     KDETIRIDGDDVVPL"
     gene            complement(20096..20395)
                     /locus_tag="ACHJ12_003233"
     CDS             complement(20096..20395)
                     /locus_tag="ACHJ12_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4990979.1"
                     /translation="MNMKKTGLTASGKPRPEFKRGDIYRDQYGGTVMIKGVAGRCVTY
                     RRKGYEYDCVMPVYQFSRDFSLVQAVPRNVPTSNVKALAKIQKMKKMINAFRGKK"
     gene            complement(20469..20783)
                     /locus_tag="ACHJ12_003234"
     CDS             complement(20469..20783)
                     /locus_tag="ACHJ12_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990980.1"
                     /translation="MRDVCNTRILEQLVTTIKAADAREARAMFDDADFYARKLLEGLA
                     ITGRLLSGMSDSIDPDMDELRSLGDSIAVTAELVAGFSEVVEVYNWRCRNGDISTDYR
                     HS"
     gene            complement(20776..21240)
                     /locus_tag="ACHJ12_003235"
     CDS             complement(20776..21240)
                     /locus_tag="ACHJ12_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4990981.1"
                     /translation="MLKNMLIASSFGLYDAGVAHMATGFSSLNTTADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSCSASLLTPLRLATMFSSVVARLLNFTTGAFHMAVS
                     ARPYFVWRFMQCHEKQASPFTATAATERETRAQLPHTHLIFVARIRQGGRHA"
     gene            complement(21233..21421)
                     /locus_tag="ACHJ12_003236"
     CDS             complement(21233..21421)
                     /locus_tag="ACHJ12_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4990982.1"
                     /translation="MATTPALVHSQTAFIWRFITFGTSERQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQGVSHA"
     gene            complement(21452..21778)
                     /locus_tag="ACHJ12_003237"
                     /pseudo
     CDS             complement(21452..21778)
                     /locus_tag="ACHJ12_003237"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(21953..22156)
                     /locus_tag="ACHJ12_003238"
     CDS             complement(21953..22156)
                     /locus_tag="ACHJ12_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4990983.1"
                     /translation="MNITEKKILTKREVANLLRLSTTSIDTMVKAGEFIPGFKRGLRR
                     VGWFEDEVKSWLEKKVEERYQQE"
     gene            complement(22294..>22764)
                     /locus_tag="ACHJ12_003239"
     CDS             complement(22294..>22764)
                     /locus_tag="ACHJ12_003239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4990984.1"
                     /translation="VVASGLLQVFNTSIDIENLSNDVTDCYVRRIVIADKGKEPKITI
                     NLYGKTINISDIFISKEEIKIVMNGGRQPGDMVKDFSDKHALSHSQSKKTTNLQARVI
                     KRLITAIAGEEEAANPRRALENGHSKLSRALTKAGVEIGATPSSVGKWLADVDD"
BASE COUNT         4991 a         6015 c         5948 g         5810 t
ORIGIN      
        1 ttaacaagaa agaaaaggca tattctctgc attatcattt tctacgactg tcaaaaatcg
       61 ctcatttttt aatgagttta tttgtttaat attatgggaa aaggtgatgc atgttggaga
      121 ggaagagtat tccccggtca ggcgaccggg gaaggggaga gattaatgtg cgcggccttg
      181 ctcaacgcca aagccggtct gggagcggat aaactgtgca cggaacagtt cacgctcgcg
      241 cgcaccttcc gctgagttat cggttgccga gaagaaccag atgccgagga atgccacggt
      301 gatagagaac agcgccgggt attcatacgg gaagatggct ttttcgtgac caaggatctg
      361 tacccaaatc gtcgggccga ggatcatcag taccactgcg gtaatcagcc ccagccagcc
      421 acccatcatc gcgccacgag tggtcagttt cgaccagtac atagaaagca gaatgatcgg
      481 gaagttacag ctcgctgcga tggcaaacgc caggcctacc atgaaggcga tgttctgatt
      541 ctcaaacagc acgccgagga taatggcaat cacacctaaa atcagcacgg tgattttcga
      601 tacccgcagt tcttcacgtt cggtcgcgcc ttttttgaag acgttagcgt acaggtcatg
      661 cgaaaccgct gatgcgcccg ccagcgtcag accagcaacc accgcaagaa tggtggcaaa
      721 agcaaccgct gaaataaaac cgaggaacag gttgccgccc actgcattcg ccaggtgaac
      781 ggccgccatg ttgttaccac caatcagatg gcccgccgcg tctttatatt ccggattcgc
      841 accaaccagc atgatcgcgc cgaagccgat aataaaggtc agaatataga agtagcccat
      901 aaatccggtg gcgtagaaca cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa
      961 gcgcatcaga atgtgcggca agcccgccgt accaaacatc agtcccagac ccagagagag
     1021 cgcggagatc ggatctttca ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg
     1081 taccgccatc gcttcactga acagattgtt gaagctaaag ccgacgtgtt tcatcaccat
     1141 aaaggccata aagctggcac cgaacagcaa cagcacggct ttgataatct gtacccaagt
     1201 ggtcgccagc atgccgccga acaggacgta catcatcatc agcacgccga ccagcaccac
     1261 cgcaatgtga tagttaaggc caaacagcag ctcgatcagt ttacctgcgc ccaccatctg
     1321 ggcgataagg taaagcgcca ccaccaccag agaaccacag gccgaaagaa tacgaatcgg
     1381 cccttgtttc agacggtaag aggccacatc ggcaaaggtg tagcgcccca ggttacgcag
     1441 acgttcggca atcaggaaca aaatgatcgg ccagcccacc aggaagccca gcgagtaaat
     1501 taagccgtca tagccggagg taaacaccag cgcggagatc cccaagaatg aggcggcgga
     1561 catatagtcc ccggcaatcg ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt
     1621 gtagtagtcg ctacgagaac gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa
     1681 cacgacgaaa atcaggaaca taataatcgc ctgccagttc gttggctggc gctctacggc
     1741 cccgctaata gcatccgcgg cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag
     1801 aactcttttc atgatgcttg tacctcatgc aggacttcat tattaagacg gtcgaattcg
     1861 ccgttcgccc gccagatgta gataccggtg agaacaaagg agatcacaat cactccaaca
     1921 ccaattggaa taccgcgtgt gacgctggtg ttcggattca gcggcgtgcc cagccagccg
     1981 ggcgcgaagg cgatcagtaa aataaagccg atataaactg ccagcataat aatcgacagg
     2041 atggtggcaa accgttgccg tttttcgact aactccctga aatgcgcatt gtcttctatc
     2101 cgctgataaa tagtgccatt catcacagat tctccagagg taatgtaggg attgttttaa
     2161 ttcccgctcc cttatgggag aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg
     2221 ataaggcgtt atatgccgca tccggcaatc aatgcctgat gcgacgctgt cgcgtcttat
     2281 caggcctaca aaccgttacc gattcgcatc cggcaattgt gggttacgat ggcatcgcga
     2341 tagcctgctt ctcttcaagc agcttctcga caacgccagg atcggcaagc gtcgaggtat
     2401 cgcccaggtt gctggtatcg cccgccgcaa ttttgcgcag aatacggcgc ataattttgc
     2461 cggaacgggt tttaggcagg gagtcggtcc agtgcagcac gtctggcgtc gccagcgggc
     2521 caatctcttt acgcacccag ttgcggactt ctgcgtacag ttctggtgac ggttcctccc
     2581 cgtgattaag cgtgacgtag gcgtagatcg cctgaccttt aatattgtgc ggaataccta
     2641 ctacggcggc ttcggcaatc ttcggatgcg ccaccagcgc cgactcaatc tctgccgtcc
     2701 ccagacggtg accggagacg ttcagcacgt cgtccacacg cccggtgatc cagtaatagc
     2761 catcttcatc gcgacgcgcg ccgtcgccgc tgaaatacat atttttgaag gtggagaagt
     2821 aggtctgttc aaaacgttcg tgatcgccaa acagcgtacg cgcctgaccc ggccaggagt
     2881 cggtgatcac caggctgcct tcggtagcgc cttccagcgg gttaccttcg ttatcgacca
     2941 gcgccggttg cacgccgaag aacggacgtg ttgccgaacc ggctttcagc tcggtagcgc
     3001 caggcagcgg cgtgatcatg aaaccgccag tttcggtctg ccaccaggta tcgaccaccg
     3061 gacatttctc gttgccgatt tttttccagt accactccca cgcttccggg ttaattggct
     3121 cgcccacgga accgagaatg cgcagcgacg aacggtcggt accctcgatc gctttatcgc
     3181 cttccgccat cagcgcgcgg atcgccgtgg gtgcggtata gagaatattg acctgatgct
     3241 tgtccaccac ctgcgccata cgggcaggcg tcggccagtt gggtacgcct tcaaacatca
     3301 gcgtggtcgc accgcaggcc agcgggccgt acagcaagta actgtgtccg gtcacccagc
     3361 ccacatcggc ggtgcaccag tagatatcac ccggatgata atcaaagaca tatttaaagg
     3421 tcagcgccgc gtacaccaga taaccgccgg tagtatgcag cacacctttt ggcttaccgg
     3481 tagaaccgga ggtgtagaga ataaacagcg gatcttcggc gttcatctct tccgcctggt
     3541 gctgatcgct cgcttgctca accaggtcgt gccaccacag gtcgcgccct tcctgccagt
     3601 caattttccc gccagtacgc ttcagtacca ccacatgctc tacgctggtg acgttcgggt
     3661 ttttcagcgc gtcatcaacg tttttcttca gcggaatact gcgcccggca cgcacacctt
     3721 cgtcggaagt gatcaccagt cgtgagttgg aatcaataat gcgcccggca acggcttccg
     3781 gcgagaagcc gccgaaaatc accgaatgca ccgcgccaat gcgggcgcag gccagcatcg
     3841 caaccgcggc ttccggcacc atcggcatat aaatcgccac cacatcacct tttttaatgc
     3901 ccagctcgag cagggtattg gcgaagcggc agacgtcgcg gtgcagctct ttatagctga
     3961 tatgtttgct ctggctggcg tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt
     4021 cttgcagatg gcggtcaagg cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt
     4081 taatggacac attaccgggg gcaaaggagg tgtttttcac cttctggtaa ggtttgatcc
     4141 agtcaagaat ttttccctgt tcgccccaga aggtatcagg tacgttaata gattgttgat
     4201 acatcgcctc gtactgctga gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg
     4261 tgtgtttgtg aatttggctc atgcttttgt tctccttgta ggatgttaat aatatgtggc
     4321 ataagcgtta aatgtagggg tattggcagt tttgtttagt atttgggcga cagatcacgc
     4381 aaaagtagaa ttgtgcaaat aaacggcagg gtaatttttg aaggtcagca acaaaagttg
     4441 attaattctt tgaggaacat gcagttatgc atgctgttga aaaagaggaa gatactgact
     4501 aactctaaag tggtatttta catgcactta caattgatta aagacaacat tcacagtgtg
     4561 gttatttgtt acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt
     4621 gaaaaacaat aaaaaccgcc attgcaacaa tggcgcaatt cggatgaagc ccctatgaca
     4681 aggataaaaa taaacgcacg ccgtatcttc agcttattga ttcctttttt ctttttcact
     4741 tctgttcacg ctgaacaaac ggcagctccc gcaaaacctg taactgtgga agcgaagaat
     4801 gaaacctttg ccccgcagca tcccgatcaa tatctctcct ggaaagccac ctcggaacag
     4861 tcagagcgtg ttgacgccct ggcggaagat ccacggctgg tgatcctgtg ggcggggtat
     4921 cccttctcgc gcgattacaa caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt
     4981 gaaaccctgc gtaccggtgc gccgaaaaac gctgaagatg gtccgctacc gatggcatgc
     5041 tggagttgta aaagcccgga tgtggcgcgt ctgatccaga aagacggcga agatggctac
     5101 ttccacggta aatgggcgcg cggcggcccg gaaatcgtca acaacttagg ttgtgccgac
     5161 tgccataaca ccgcctcacc agagttcgcc aaaggcaaac cggagttaac cctttcccgt
     5221 ccgtatgcgg ctcgcgcgat ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc
     5281 gaccagcaat cgatggtttg cggtcagtgc catgtggagt attacttcga cggcaaaaac
     5341 aaagcggtta aattcccgtg ggatgacggc atgaaagtcg aaaatatgga gcagtattac
     5401 gacaaaattg ccttctctga ctggactaac tccctgtcga aaacgccaat gctgaaagcg
     5461 cagcacccgg aatatgaaac ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt
     5521 atcgactgcc atatgccaaa agtgcagaac gccgaaggca aactctacac cgaccataaa
     5581 attggtaatc cgtttgataa cttcgcccag acttgtgcga actgccatac ccaggacaaa
     5641 gctgccttgc aaaaagtggt cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag
     5701 gttgaagatc aactggttca cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg
     5761 acggaagctg aaatgaagcc aattcaggac gatatccgtc atgcccagtg gcgctgggat
     5821 ctggcgatcg cttcccacgg cattcatatg cacgcaccgg aagaaggttt acggatgctc
     5881 ggtacggcga tggataaagc ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc
     5941 aaaggcatca cccatgaaat ccagatcccg gatatctcga ccaaagagaa agcccagcag
     6001 gccattggcc tgaacatgga acaaatcaag gccgagaagc aggacttcat caaaacggtg
     6061 atcccgcagt gggaagaaca ggcacgtaaa aatggtctgt taagccaata accccgttcc
     6121 gcctcgcaag gggcggaaaa cacaatggag tgaatatgag cgtattacgt tcgttattaa
     6181 ctgctggggt gctggcgtcg ggcctgttgt ggagcctgaa cggaattacc gctacccctg
     6241 cggcgcaggc atctggtgat cgctacgaag ttacccagca gcgtaacccg gatgccgcct
     6301 gtctggactg tcataagcca gatatcgaag gtatgcatgg caaacatgct tccgttatca
     6361 acccgaataa caaactgccg gtcacctgca ccaactgtca cggccagcca tcaccgcaac
     6421 accgcgaagg ggtgaaagat gtgatgcgct ttaacgagcc gatgtacaag gtcggagagc
     6481 agaacagcgt ctgtatgtcc tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc
     6541 acgatgtcca cgtaaccaaa gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag
     6601 atacgatgca gacgttaagt gacaaaggac ggattaagat ttgcgtcgat tgccacagcg
     6661 atcagcgcac caatccgaac tttaacccgg cgtctgttcc gttgcttaag gagcagccat
     6721 gacctggtct cgtcgccagt ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac
     6781 cgcagggcgc gtggtggcga aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca
     6841 tgacgagtcg ttatgcatcg gctgtaccgc ctgtatggat gcttgtcggg aagtgaacaa
     6901 ggtgccggaa ggcgtctcgc gcctgacgat tattcgtagc gagccgcagg gcgaatttcc
     6961 tgatgtgaaa tatcgcttct tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt
     7021 tgacgtctgc ccgaccggtg cgtcttttcg tgatgccgct agcgggattg tcgatgttaa
     7081 tcctgatctc tgcgtcggtt gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt
     7141 tatccatccg gtcacgaaga cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca
     7201 ggccggtaag ctgcccgcgt gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa
     7261 tctggacgat cctaacagtg agatttcgca actgctgcgc cagaagccta cttaccgcta
     7321 caagctggcg ctgggaacca aaccgaagct gtaccgcgta ccgtttaaat acggggaggt
     7381 gagccaatga cgcagacttc cgcatttcat tttgaatcgc tggtgtggga ctggccgatt
     7441 gccatctacc tgtttttgat tggtatttct gccggtctgg tgacgctggc cgtgctgtta
     7501 cgtcgcttct acccgcaggc gggcggtgca gacagtacgt tgctgcgcac cacgctgatt
     7561 gtcgggccgg gcgcggtgat cctcggtctg ttgatcctcg tcttccacct gacaagaccg
     7621 tggaccttct ggaagctgat gttccactac agttttacct cggtgatgtc gatgggggtg
     7681 atgctgtttc agctctacat ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat
     7741 gatttgcttg ccctgcaaca acgctggttg ccgaagctgg ggatcgtgca aaaggttctg
     7801 agcctgctga cgcccgttca tcgcggactg gaaacgctga tgctggtgtt ggcggtgctg
     7861 ctgggggctt ataccggctt tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac
     7921 ccgatcctgc cggtgctgtt cctcttctcc ggcatctcgt ccggtgtggc ggtagcgctg
     7981 atcgccatgg cgatacgcca acgcaaaaat ccgcattcca cggaagcgca ctttgttcac
     8041 cgtatggaaa tccctgtggt atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt
     8101 ctggccctgg gcgatgacgg taaagtgcgt gcgctggtgg cggcattagg tggcggtttc
     8161 tggacgtggt ggttctggct tggtgtcgcc gggctggggc tgattgtgcc aatgctgctc
     8221 aaaccgtggg tcaatcgcag ttccggcatt cctgccgtgc tggcggcgtg tggggccagt
     8281 ctggtcggcg tgttgatgct gcgctttttc attctctacg ccggacagtt gacggtggcg
     8341 taagccagaa aagaggtggt ttctggacgt attccttcct gaagtcggtt ttctggcgtt
     8401 gttgttaagt ctcggggtca acgtgttgac cccgttgacg gccttcgcgg gagtgcggtt
     8461 gcgctggcct gccatgatgc gactcacttg catcggcatt ctggcacagt tcgcgctcct
     8521 gctgctcgcc tttggcgtac tgacgtattg ttttctcatc agcgatttct cggtcattta
     8581 tgtcgcgcaa catagctaca gcctgctgtc gtgggaactc aagctggcag cggtgtgggg
     8641 cggtcatgaa ggttcgctgc tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt
     8701 tgcctggcat tatcggcagc aaaccgatcc gctatttccg ctgacgctag ccgttttatc
     8761 tctcatgctc gccgcactgc tactgtttgt ggtgctgtgg tccgatccct tcatgcggat
     8821 atttccacca gcaatcgaag gccgcgatct caatccgatg ctgcaacatc ccggtcttat
     8881 cttccatccg ccgctgcttt atctcggcta tggcggtttg atggtagcgg cgagcgtggc
     8941 gctggcgagt ttactgcgcg gcgagtttga tggtgcctgc gccagaattt gttggcgctg
     9001 ggcactacct ggctggagcg cattaacggc ggggatcatc ctcggttcct ggtgggccta
     9061 ttgcgaactg ggctggggcg gctggtggtt ctgggatccg gtggaaaacg cctctttatt
     9121 accctggctt tctgccactg cgctgctgca cagtttgtcc ctgacacgcc agcgggagat
     9181 tttccgccac tggtcgctgt tgctggcgat agtaactctg atgctgtcgc tactgggtac
     9241 cttaattgtc cgttctggca ttctggtttc ggttcatgcg ttcgcactgg ataacgtccg
     9301 cgccgtgccg ttgttcagcc tgtttgcact gattagcctt gcgtctctgg ctctgtatgg
     9361 ctggcgagcg cgggacggtg gcccggtggt gcgtttttcg gggttatcgc gggaaatgtt
     9421 aatcctcgct acgctgttgc tgttttgcgc agtgctactg atcgtgctgg tgggaacgct
     9481 ttatccgatg atttacggtc tgctgggctg gggacgcctc tccgttggcg cgccgtattt
     9541 taaccgcgcg acgttaccgt ttggcctgtt gatgctggtg gtgattgtgc tggcgacgtt
     9601 tgtctctggc aaacgcgtgc agcttccggc gctggtagct catgcgggcg tgctgttatt
     9661 tgccgcgggg atcgtggttt ccagcgtcag ccgtcaggag atcagcctga atttacagcc
     9721 gggtcagcag gtgacgctgg caggatacac cttccgtttt gagcgcctcg atctgcaagc
     9781 caaaggcaat tacaccagcg aaaaagcgat agtggcactg tttgaccatc agcaacgcat
     9841 tggtgaactg atgccggagc ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc
     9901 gtcaattcgc tggaacggca tccatgactg gtatgcggtc atgggtgaaa aaaccggagc
     9961 ggatcgttac gcttttcgct tgtatgtaca aagcggtgtg cgctggatct gggggggagg
    10021 attgttgatg attgcgggcg cattgttaag cggatggcgg gggaggaagc gcgatgaata
    10081 aaggacttct cacgctactg ctgttattta cctgttttgc gcgcgctcag gtcgtagaca
    10141 cctggcaatt cgccaatccg caacaacagc aacaggcgtt aaatattgcc agccagttac
    10201 gttgtccgca gtgccagaat caaaacttac tggaatccaa cgcgccggtg gcagtcagta
    10261 tgcgccatca ggtttacagc atggtggcgg aggggaagaa cgaagtcgaa atcattggct
    10321 ggatgaccga acgctacgga gattttgttc gctataaccc accgttaacg ggtcagacgc
    10381 tggtgttgtg ggcgctgcca gtggtgttgt tactgctgat ggcactgatc ctctggcgag
    10441 tgagggcgaa gcgatgaaag agcccaaaat accggtgaaa atgctgacaa cactcacgat
    10501 tttgatggta tttctctgtg tcggcagtta tctgttaagt ccaaaatggc aggcggtaca
    10561 tgcggaatat cagcgtcagc gcgatccgct acatcagttt gccagccagc aaaccccgga
    10621 agcgcagctt caggcattgc aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg
    10681 ggcgttactg ggcgagtact atctgtggca aaacgattac agcaattcgc tgctggcgta
    10741 ccgtcaggcg ttgcaactac gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt
    10801 gctttattac caggccagcc agcatatgac cgcccagact cgcgcaatga tcgacaaagc
    10861 cctcgcgctg gactctaacg aaatcaccgc tctgatgctg ctggcttccg atgcgtttat
    10921 gcaggcgaat tacgcgcaag ccatcgaact atggcaaaaa gtgatggatc tcaactcacc
    10981 ccggatcaac cggacacagc tggtcgagtc gattaatatg gcgaaattgt tgcagcggag
    11041 atcagattaa tcatctggtg atgaagatta tgttattgag ttgtataaaa aataatcgaa
    11101 tgaacatatg ccaaaaataa tcaccaatca gtattattgc agattaaaca aataaaaatc
    11161 tttccataac aaatggttat tcattaatcc tgccataaac tcgcgttatt atgcattaat
    11221 gcagcgaaaa gctctgttgt taaagggttg cgcaacatac cgcgcaaatg atactgatca
    11281 taagcgttaa aaaaatctac aaaccaacgc aacacaattc atgccctggc agtatgtcac
    11341 gttctcgcgt ttctgaacgg ggaacggcgc tccattgagg aagttattct gatgaaaagt
    11401 ataaaattca gcctggcctg gcagattctg tttgctatgg tgctgggcat tctcctggga
    11461 agctacctgc actaccatag cgacagccgc gactggctgg tcgtcaattt gctctctccg
    11521 gcgggtgata tcttcatcca tctgatcaaa atgattgttg tgccgattgt gatctccacg
    11581 ctggtggtgg gtatcgcggg tgttggtgat gccaaacagc tcgggcgtat tggcgcgaaa
    11641 accattattt acttcgaggt gatcaccacc gtcgccatca ttttggggat cactctggcg
    11701 aacgtcttcc agcccggtgc cggggtggat atgtcgcagc tggcgaccgt cgatatctcg
    11761 aaatatcaga gcactacgga agcggtacaa agcagttccc acggcattat gggcacgatt
    11821 ttgtcgctgg tgccgacgaa cattgtggcg tcgatggcga aaggcgaaat gctgccgatc
    11881 atcttcttct cggtgctgtt tggtctgggg ctttcctccc tgcccgcgac gcatcgtgaa
    11941 ccgctggtga ccgtgttccg ctccatctct gaaaccatgt ttaaagtgac tcacatggtg
    12001 atgcgttatg caccggtggg tgtgtttgcg ctgattgcgg tgacggtggc taactttggt
    12061 ttctcttctc tgtggccact ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc
    12121 ttcgcgctgg tagtgctggg aattgtggcg cgcctgtgcg ggttaagcgt ctggatcctg
    12181 attcgtattc tgaaagatga gctgattctg gcgtactcca ctgccagctc tgaaagcgta
    12241 ctgccgcgaa ttattgagaa gatggaagcc tacggagcac cggcgtcgat caccagcttc
    12301 gtggtgccga ccggttactc ttttaacctt gatggttcga cgctgtatca aagtattgcc
    12361 gctattttca tcgctcagct gtatggcatt gacctgtcca tctggcagga aatcattctg
    12421 gtgctgacgc tgatggtgac ctcgaaaggg attgctggcg tgccgggcgt gtcgtttgtg
    12481 gtgctgctgg caacgctggg tagcgtaggt attccgctgg aaggcctggc gtttattgct
    12541 ggtgttgacc gtatcctcga catggcgcgt actgcgctga acgtggtggg taatgcgctg
    12601 gcggtgctgg tgattgccaa gtgggaacac aaatttgacc gcaaaaaggc gctggcttat
    12661 gagcgtgaag tgctgggcaa atttgataaa actgcggatc aataattgaa gattgccggg
    12721 gttatccacc cggcaatgtg tggatgcctg atgcgacgct ggtgcgtctt atcaggccta
    12781 ccttaaccgt tggttaattt ttcaaactct tcacacccgg tatcaaaccc ttccatacag
    12841 ctcaggttca accagtgcag cgccttttgt ttgttcttct cgataaaacc ttcctcaccg
    12901 tttaagaaca tcattcccgc ccagtactcg gaataaccgg ttcgggaaat tgcagagctg
    12961 cgtttgaaat accaggttgc cttgtcatcg tcggccttaa tgcccacgcc gttggcgtaa
    13021 atcaaaccaa gcagcatttg ggcatcgacg gcggagtcat tctccagatc ttccgaggcg
    13081 ttttctaaca gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga
    13141 atatgcgcca gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga
    13201 acttttgctt gtggatagtc cagactgacc ggattggtga ttttgatctg cgccagcagc
    13261 gcgcaggcat cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc
    13321 aaatcgccgg agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc
    13381 tctgctgcct ttaaatactg gctgccaagc tcggagtcgt tcgcgtgggc aaagaatgtc
    13441 aaaaacaaca tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa
    13501 ccagtataaa gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga
    13561 ctgtattacg ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc
    13621 acgtactgct cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg
    13681 tattccggcg ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggcacca
    13741 atccaccact ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca
    13801 cgggtgataa ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc
    13861 agacgtttgg cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc
    13921 agtgccgcac agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt
    13981 gacagtacca tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg
    14041 aagaactgtg ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc
    14101 tgatcggcat ctgaagtatc gcggcaaggc cactgaatga agcccagttc gcccattttc
    14161 tcgtaagtcg caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg
    14221 gtgttgttgt agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag
    14281 tccgttttca gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca
    14341 gccgcagtaa acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc
    14401 gccgacgcgg ttttggtcat aaagatgtcc tgaacgataa ccagttccag atcttcaaag
    14461 gctttacgta ctgccgacag ctccgcgtca gtttgcagcg gatcttcgcc cataatgtac
    14521 gcggcacgca cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg ataaccggta
    14581 tgagcaggca ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct
    14641 ttcacgtact ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc
    14701 acgttgttct gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc
    14761 agcatcgcga ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca
    14821 cccatgcccc acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga
    14881 atctcactgg cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg
    14941 attttacgat actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga
    15001 ttttcttcaa taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc
    15061 agtgcaatgt gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata
    15121 attttcgccc cgttacgttt agcgttaatt acgtgattcg ccacgattgg gtgggaatcc
    15181 gccgggttgt acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg
    15241 ctcattgcgc cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca
    15301 cgagcgcagc agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca
    15361 tagttggttt cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg
    15421 tacttctctt tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa
    15481 acaggttcga gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc
    15541 aggatctggg tatcgttaat gaaatcccag ccataataac ccttcagaca cagggtaccc
    15601 tggttggttt tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg
    15661 ttgattttgc aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc
    15721 gctccagtta atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca
    15781 cattttattt ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa
    15841 aaacaggctg tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc
    15901 ggcagacatt ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt
    15961 tcatcaactg aatgctttgg atcacccggc ggctgtccag catcagtaat gacatctctt
    16021 cggcaagtac tggcaaccgg gcttcagtgg cctgtaagcg gctggttaag ccgcgctgat
    16081 aggcggcctc ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt
    16141 ctcgctcgtc gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt
    16201 tcagtactga ctggttgtaa cgttcaatca tcatgttgct ggcggcgcgc gtgccttcga
    16261 gattggcatt caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgtctttga
    16321 actgaatgta tattttgttg ataaataaag attatgtgtt tttgattttc gttttgtatg
    16381 cctttttgta tgcctttttt gaaaaagata cccttgttgt ttgatcgtcg ataacgggta
    16441 tcaatgtgca catgctaccc gatggcgttc atgtgtgcgc tgtcgaacgt ttgaaaatgg
    16501 ttttagggcg gttttaatgc tggttgatgt ggcgctctgt ggcgctcgtt ttggtgtgct
    16561 ggtggcgggg gtcgaacccg catacatgat cagcctgtac aggctggcac cgactctacc
    16621 cgttgagcgt acaccagcaa aaaagcgatg aggttgttgg ctgctggtgg tgggggtcga
    16681 acccacataa acatcccgct ttctggaagc tatacaagcg ggtgccgctt taccgattaa
    16741 gagtacacca tacagccagg caaaatagta atcatgcgtt atgtgcgggt aaagtctgtt
    16801 tcgtctggta gcaccagatg aacgggtatc attatttcac ctgatttcac gtgatttcgc
    16861 ttaatttcac aaaaatcacc ttctttcacc ttctttcacc ttctttcaca aaaaaacaaa
    16921 tcatcattaa agcgcctgat tcgctctctg atgcgttttg gtgttttgtg gcgtccgtga
    16981 tgccctgaaa ggggattttt tgcgatagcg gcgcaaatat aacggttgct gctggtatct
    17041 gtggttagtc ttgcgtgatt ttattggcgt gcttctttct caggcgtcgt atgatgtcgt
    17101 aaatatgggt ttcggatatt gcgtactctt tgcgtaatgt ctgcaatttt tcgccgttat
    17161 cccacctgta gtaaatctcg tattcgcgca atgctgcgaa aagtgatctg cctgttggaa
    17221 ggtaaagact tcgcccacca gcatagaagg cgattgcagc gacaacccgc tttgcgattc
    17281 tgaaagcgtc tgcctcctcc atgccattac gggtaagctc cgctactgaa tgcgacacga
    17341 tatcagcaag cgaatcacac catgcatcac ctggtttatc tccgttgtct atggcgtcga
    17401 tgaatttaag ccgctcgatg tcttttggta cgtgatgggt ttgctcgttc atccggtagc
    17461 ctcttgcgtt ggtgtatctg gtcctaccag tttaatttaa cgtcgcaata aaacgatata
    17521 cacgcacaca atcagaaaaa ccgtctgact gttccggtcc tgtgcttact tctggtagct
    17581 acgccagatt tttttacatc tccctcctat ccccatttct gcgtatgtgc ggtttactgc
    17641 tttcctcagg tctgcgtagt tctcctctgg cggctccggt ggcctctgtg ccttcccgta
    17701 acactctgcc ctgcgcattg ccacccgttc gcgttccctg tctgtctcca ccagcatcat
    17761 cacttcggcc catcgcgccg ccgttctccg gtaaaagcct ttcgactcca gttcttccgc
    17821 tatgcggtca tgtaccatcg ttctcccctc agaacggaat atcatcacca taagggtcat
    17881 cgcctcccgc tggtggctga ttaccctgtg tgcctgtggt cctgcgtctg ctgccacctg
    17941 gttgcgccgt tttcgcactg attacgctgt ctgcaataac ctgataaccc tgccgcgttt
    18001 ccccgttctg tccggtccac tggctgacct gcatcacgcc ggatacgctg attacgttgc
    18061 ctttctcatg gtgtgccagt tggtcggcct gtttgccaaa tgccaggacc gacagccata
    18121 acgtcgcctg cccgtcctgc gcctgactgc atggcagtga taccgccata cgcgccagcg
    18181 tcatcggtgt gcccttactg gtctgttttg tctgcgggtc gtcaatcagt cgcccgtaag
    18241 ctgctatctg tgctgtcatg attccacctc tccggtttta acgttgatgg ttgttacctg
    18301 ttccgcttct gcaatctccc gctctgtcag cgtggtaaag tttgtcgctg ctgtgctcat
    18361 gaatgcactt atcagccagg gggctttcgc gtgtctttct gccgtgttgt ggcccttcgt
    18421 tcacgagcaa aacacttaaa taaaaataaa gtgattttct gtgttttcgt gctgctctgc
    18481 tctgtgtttt tttgccgttt actcaccact actcactttt tttactcact ttttggaatc
    18541 gctcacttcg tctcacctga gatttttggt gtttttatgt gtatgtttta taagtggttt
    18601 tttactgctc acttttttgc ctatatacag gtgagaaagt gagtaatttt gttaattttt
    18661 tgtaaaatta ctcactttac tcacttttgc tcctcacttt tttacagcgg tactacgtca
    18721 tcgccgtcaa tgcgtatagt ctcgtctttc tccagttttg ctaaccatcg ccgcatgttt
    18781 ttcacgtcat acccaagccg tttcatgtcg tcgcgcaaca acgaaatagt gcacttgtcg
    18841 ccgtgctgtg tgcgtgaccg gatgcacccc catagcgcgg tatggttttc cgtcttgttc
    18901 cctgcctcct cgatgcgctc ctgttcaacg ggaggacgcg gcttatcaac caccaccagc
    18961 gacgtaatta actccccgtc agcgtcggta aaaagctcca ccacgcgtaa gtcatatgct
    19021 gcttctttga gttcctccgc gtccttcatt ttggtacatg agataaccag cgcttcgctg
    19081 cctgcgtcct ccctgcgtat ccggtattcg gcgtccagtg atgcacgaaa tgcactggaa
    19141 ccgcgcgcgc ctttcgtctc atccttgccg gaatggtgaa ccaccagcac cgtggcccct
    19201 gtgcgccgtt taagctcgtc acacccacgg ataaacgccc ccatatcacg ggaatcattt
    19261 tcatcattgc cgccaaagca acgcgccagc gtatccagaa taatcatgcg tacaggttta
    19321 cccgtttccc gctccacctg gcgggcagcg ataaccattt catcaacatc aagcgggaca
    19381 gccggaaaga tgggacggtt taccagatac agatttttca cctgctcacc gtgcacaacc
    19441 tcccaggctt ttacacgacg cggaacgccg atacctcctt caccaaccac atagagaacc
    19501 gcaccatgcg ccaccctgcg gcctccccac tggcgccccg tggaaacgtg acacgcccac
    19561 gaacccgcaa ggaacgattt ataggaaccg ctcgccccgt atatgctgca tagcgacgat
    19621 gccggaataa tcccctttac cacgtaatca agttgtgtat cgtatccggc agaaccaacg
    19681 ctcatcggta gctttgtttt gcgctggtgg cctttttctt cctgcttttc cccgcgctcc
    19741 cgttccagat attcgcgcca gttctcccgc ttctggctgt gcatcccatc ggggtaataa
    19801 ttcgcatcct gtacacctgc cgctgccagc ttgtgcgcaa tggcattaat atttgatggt
    19861 ctgatatggc ctgccttgta cagccggacg caatagcggc cctcgtcgat gattctcatg
    19921 tctgtcagtt ctgccagttg ctgctcccca agccatacag gtggcacatt atcgccagca
    19981 agtcggccct cctgctcctg ccactgcttc gcgtgtgccc aggcatcact acccgcaaaa
    20041 ataatgacct cggtcatttt gtcgtaaggc tgttttttta agttcggtgc gcttttcatt
    20101 tcttgcccct gaatgcgtta atcatctttt tcatcttctg tatttttgcc agtgccttta
    20161 cgttgctggt gggcacgtta cgcggtaccg cctgcaccag agaaaaatca cgggaaaact
    20221 gataaacagg catcacgcaa tcatattcgt agcctttacg gcggtaggtg acgcaccgcc
    20281 ccgccacgcc cttaatcatt accgtgccgc cgtactggtc gcggtaaata tcaccgcgct
    20341 taaattcagg gcgaggcttg ccgctggcag ttaagccagt ttttttcatg ttcatttttt
    20401 tatcctttgt tctggtcagt tacgccgttg ctgcataaat gcggttagct gtgactgtcg
    20461 ttgccttatc acgaatggcg ataatcagta ctgatatctc cgttacggca acgccagttg
    20521 tacacctcca caacctcact gaatccggcc acaagttcag ccgtaacagc gatggaatcg
    20581 cccaggctgc gcagttcgtc catgtcagga tcaatgctgt cactcatgcc agaaagtaag
    20641 cgccctgtga tagccagccc ttcaagcaat ttgcgggcgt agaagtcagc atcatcaaac
    20701 atcgctctcg cttcccgtgc gtcagctgct tttatggttg taaccagttg ttcaagtatt
    20761 ctggtgttac acacatcacg catgacgtcc cccctgacga atacgggcga caaaaatcag
    20821 atgggtatgc ggaagttgtg cgcgggtttc gcgttctgtt gctgccgtgg cggtaaaggg
    20881 tgaggcttgt ttttcatggc actgcataaa gcgccagaca aagtaagggc gtgcggatac
    20941 agccatgtga aaggctcctg tagtgaaatt aaggagtctc gctactacgc tgctaaacat
    21001 ggtggcgaga cgtaacgggg ttagcagact ggcactacag gaaccagcga gcgcaaaggc
    21061 tcccccgtta cgccccgcca taatgcgggt atgggtaggt ttacggacgc aaaaaaaccg
    21121 catatcggaa tgtaagcggc tgtccgctgt agtattcagg ctgctaaacc cggtcgccat
    21181 gtgggcgact ccggcatcat acagcccgaa agaagaggcg atcaacatgt ttttaagcat
    21241 gactcacccc ctgacgaata cgggcagcga atacagcgac acaaccggac gggcaacggt
    21301 tacgcgcttc gcgttccgtc caggcggtta cgtggataat ttgacgttct gacgtgccaa
    21361 aggtgataaa acgccagata aaggcagtct gagaatgtac gagggctggg gtagtagcca
    21421 tgatggcagc ctcctttatc gttgggatat acccaccgct ggaggttcca atctcgctgg
    21481 cggtggactg cacggagttg gaactactgg cgataaagga aaccagcctg accgaagtca
    21541 gccccataca gcccaccatt gataaggtgt gcgtgtgcgc cgatacaaaa aaagacgcgt
    21601 tcggcgtctg tatcgccttt atctccagcg gggttccaat cccgacaccc gttttataag
    21661 gtgcgcggga atcatagcca tttttgatac tggcgatcaa ctgattttct ctggcgggta
    21721 tttggcgcgg atgcctgttg cctgtgacaa tatgcacacg actgtataca atacacatcg
    21781 gttaactcct tatattacgt taatggcccg tagtgttatt gcattgcggg tattttttta
    21841 cctgccgtta attgaatgcg gcaaggcatc acgatggtta acccgttttg cataacgtga
    21901 ggcgttttat tttctgcgct ggtggctttt atatgtcaca catggaaatt tattattcct
    21961 gctgataacg ctcctctact ttcttctcaa gccatgattt cacctcatcc tcaaaccagc
    22021 caacgcgacg caatccacgc ttaaagccag gaataaactc accagcctta accattgtgt
    22081 caatgcttgt tgtactaagg cgaagaagat ttgcgacttc tcgttttgta aggattttct
    22141 tttctgtgat gttcatgctt tgctttcctt ttgtttcagg cgataacttg cctaaacttg
    22201 cttaaggata tgcaaacatg gttaatgtcg gtattgcgca atattacgaa aaaagcccgt
    22261 aatattacgg gcggtcatgt gttcagaagt ctgtcagtcg tcaacgtctg caagccactt
    22321 accaacagat gaaggggttg cgccaatttc aacccctgct ttggttagtg cgcggcttaa
    22381 cttgctgtga ccgttctcaa gcgctcttct tgggtttgct gcctcttctt ctccggctat
    22441 ggcggtgatt agtcttttga taactctggc ctgaagattg gttgttttct tactctgcga
    22501 atgcgacaag gcgtgtttat ctgaaaaatc ctttaccata tctcctggtt gtctgccgcc
    22561 gttcattact atttttatct cttcttttga gatgaaaatg tcactaatat ttattgtttt
    22621 gccatataaa tttattgtta tctttggttc ttttccttta tctgctataa cgatgcgtcg
    22681 cacataacaa tctgtgacgt catttgatag attctctata tcaatgcttg tattaaatac
    22741 ctgtaaaagt cctgaagcca caac
//
LOCUS       DAZFFI010000072        22707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFFI010000072 DAZFFI010000000
VERSION     DAZFFI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.072"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            113..655
                     /gene="yehD"
                     /locus_tag="ACHJ12_003240"
     CDS             113..655
                     /gene="yehD"
                     /locus_tag="ACHJ12_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4990985.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            736..1410
                     /gene="yehC"
                     /locus_tag="ACHJ12_003241"
     CDS             736..1410
                     /gene="yehC"
                     /locus_tag="ACHJ12_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4990986.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVSRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1426..3906
                     /gene="yehB"
                     /locus_tag="ACHJ12_003242"
     CDS             1426..3906
                     /gene="yehB"
                     /locus_tag="ACHJ12_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4990987.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLVNRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3920..4954
                     /gene="yehA"
                     /locus_tag="ACHJ12_003243"
     CDS             3920..4954
                     /gene="yehA"
                     /locus_tag="ACHJ12_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO4990988.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(5036..5374)
                     /gene="rcnB"
                     /locus_tag="ACHJ12_003244"
     CDS             complement(5036..5374)
                     /gene="rcnB"
                     /locus_tag="ACHJ12_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4990989.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5593..6417)
                     /gene="rcnA"
                     /locus_tag="ACHJ12_003245"
     CDS             complement(5593..6417)
                     /gene="rcnA"
                     /locus_tag="ACHJ12_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4990990.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6538..6810
                     /gene="rcnR"
                     /locus_tag="ACHJ12_003246"
     CDS             6538..6810
                     /gene="rcnR"
                     /locus_tag="ACHJ12_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4990991.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6902..6998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7033..7821
                     /gene="thiM"
                     /locus_tag="ACHJ12_003247"
     CDS             7033..7821
                     /gene="thiM"
                     /locus_tag="ACHJ12_003247"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4990992.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7818..8618
                     /gene="thiD"
                     /locus_tag="ACHJ12_003248"
     CDS             7818..8618
                     /gene="thiD"
                     /locus_tag="ACHJ12_003248"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4990993.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8683..9501
                     /gene="yegX"
                     /locus_tag="ACHJ12_003249"
     CDS             8683..9501
                     /gene="yegX"
                     /locus_tag="ACHJ12_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4990994.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            9553..10299
                     /gene="yegW"
                     /locus_tag="ACHJ12_003250"
     CDS             9553..10299
                     /gene="yegW"
                     /locus_tag="ACHJ12_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4990995.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVLIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10273..11238)
                     /gene="yegV"
                     /locus_tag="ACHJ12_003251"
     CDS             complement(10273..11238)
                     /gene="yegV"
                     /locus_tag="ACHJ12_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4990996.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11235..12239)
                     /gene="yegU"
                     /locus_tag="ACHJ12_003252"
     CDS             complement(11235..12239)
                     /gene="yegU"
                     /locus_tag="ACHJ12_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4990997.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12236..13513)
                     /gene="yegT"
                     /locus_tag="ACHJ12_003253"
     CDS             complement(12236..13513)
                     /gene="yegT"
                     /locus_tag="ACHJ12_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4990998.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13770..14822
                     /gene="fbaB"
                     /locus_tag="ACHJ12_003254"
     CDS             13770..14822
                     /gene="fbaB"
                     /locus_tag="ACHJ12_003254"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4990999.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            15130..15984
                     /gene="gatY"
                     /locus_tag="ACHJ12_003255"
     CDS             15130..15984
                     /gene="gatY"
                     /locus_tag="ACHJ12_003255"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4991000.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            16013..17274
                     /gene="gatZ"
                     /locus_tag="ACHJ12_003256"
                     /pseudo
     CDS             16013..17274
                     /gene="gatZ"
                     /locus_tag="ACHJ12_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            17284..17736
                     /gene="gatA"
                     /locus_tag="ACHJ12_003257"
     CDS             17284..17736
                     /gene="gatA"
                     /locus_tag="ACHJ12_003257"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4991001.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETKLKEYFTKYVLDSDE"
     gene            17767..18051
                     /gene="gatB"
                     /locus_tag="ACHJ12_003258"
     CDS             17767..18051
                     /gene="gatB"
                     /locus_tag="ACHJ12_003258"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4991002.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            18055..19410
                     /locus_tag="ACHJ12_003259"
     CDS             18055..19410
                     /locus_tag="ACHJ12_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4991003.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            19458..20498
                     /gene="gatD"
                     /locus_tag="ACHJ12_003260"
     CDS             19458..20498
                     /gene="gatD"
                     /locus_tag="ACHJ12_003260"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4991004.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPDDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            20604..21377
                     /locus_tag="ACHJ12_003261"
     CDS             20604..21377
                     /locus_tag="ACHJ12_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4991005.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21459..22358)
                     /gene="yegS"
                     /locus_tag="ACHJ12_003262"
     CDS             complement(21459..22358)
                     /gene="yegS"
                     /locus_tag="ACHJ12_003262"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4991006.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         5982 a         5220 c         5760 g         5745 t
ORIGIN      
        1 aatcaataaa acaatgtgtt tacattgcgg caactttttt gatgtgagaa atttgtttcc
       61 aagagataca gactcttaac aaccaaaaat aaattttatt aaggaacata ttatgaaacg
      121 ttcaattatt gctgccgctg tcttttcttc tttttttatg agcgctggag tatttgctgc
      181 agacgttgat accggaacat taactattaa ggggaatatt gcagaatctc cgtgtaaatt
      241 cgaagcgggt ggtgattcag taagtattaa tatgccgact gtaccaacca ctgtctttga
      301 aggtaaagct aaatattcta cctatgatga tgcagtcggt gtaaccagca gcatgttaaa
      361 aattagctgc ccgaaagaag ttgctggtgt aaaactctcg ttgattacca acgataaaat
      421 aaccggtaac gataaggcga tagccagtag caacgatacc gtgggttact atctctattt
      481 aggtgataac agcgatgtcc tggatgtttc tgcacctttt aacattgaga gttataaaac
      541 agcggaaggt caatatgcta ttccgtttaa agcaaaatac ctgaaactga cagataactc
      601 agtgcaatca ggtgatgtgt tatcttctct ggttatgcgt gtggcgcagg attaataaat
      661 aaaattgcaa gtttctccag gtaataaaca tggccgctat ccccatggcg gctttttaat
      721 ttaagaggca ttaagatgaa aggattatta tctttactca ttttttctat ggtccttcct
      781 gcacatgccg gaattgttat ctacgggacg cgcattattt acccggcaga aaataaagaa
      841 gttatggtgc agttgatgaa ccagggaaac cgttcttcgc tgctgcaggc gtggattgat
      901 gatggcgata cgtcgttacc accagaaaaa attcaggttc ctttcatgtt aacgccacca
      961 gtggcaaaaa taggggcaaa ttccgggcag caagtaaaaa tcaaaattat gccgaataaa
     1021 ctgcccacta ataaagaaag cattttttat ctgaatgttc ttgatattcc accaaatagt
     1081 ccagaacaag aaggtaagaa tgcactgaag tttgcgatgc aaaacagaat caaattgttt
     1141 taccgcccgg cgggtattgc tccggtaaat aaagcgacct ttaaaaagtt actggtaagt
     1201 cgcagtggca atggattggt gataaaaaat gactcagcta attgggtgac gatttcggat
     1261 gtcaaagcta ataatgtcaa agtcaattat gaaactataa tgattgcccc cttagaaagt
     1321 cagagtgtta atgtcaaaag taataatgca aataactggt atctgaccat tatcgatgac
     1381 cacggcaact atattagtga caaaatttaa ttgcaggagc tgcctatgtt gagaatgacc
     1441 ccacttgcat cagcaatcgt agcgttattg ctcggcattg aagcttatgc agctgaagaa
     1501 acctttgata cccattttat gataggtgga atgaaagacc agcaggttgc aaatattcgt
     1561 cttgatgata atcaaccctt accggggcag tatgacatcg atatttatgt caataagcaa
     1621 tggcgcggga aatatgagat tattgttaaa gacaacccgc aagaaacatg tttatcaata
     1681 gaagttatca agcggttagg cattaatagc gataacttcg ccagcggtaa gcaatgttta
     1741 acatttgagc aacttgttca gggtgggagc tatacctggg atatcggggt ttttcgtctc
     1801 gatttcagtg tcccgcaggc ctgggtggaa gaactggaaa gtggctatgt tccaccggaa
     1861 aactgggagc ggggtattaa tgcgttttat acctcttatt atgtgagtca gtattacagt
     1921 gactataaag cgtcgggtaa tagtaagagt acatatgtac gttttaacag cgggttaaat
     1981 ttactggggt ggcaactgca ttctgatgcc agtttcagta aaacaaataa caatccaggg
     2041 gtgtggaaaa gcaataccct gtatctggaa cgtggatttg cccaacttct cggcacgctt
     2101 cgcgtgggtg atatgtacac atcaagcgat atttttgatt ctgttcgctt cagcggtgtg
     2161 cggttgtttc gtgatatgca gatgttgcct aactcgaaac aaaattttac gccacgggtg
     2221 caggggattg ctcagagtaa tgcgctggta actattgaac agaacggttt tgtggtttat
     2281 cagaaagagg ttcctcctgg cccgttcgcg attacagatt tgcagttggc cggtggtgga
     2341 gcagatcttg atgtcagcgt gaaagaggcg gacggctcgg taaccaccta tctggtgcct
     2401 tatgcagcag tgccaaatat gctgcaaccc ggcgtatcga aatatgattt tgcggcgggt
     2461 cgtagccata ttgaaggggc gagcaaacaa agtgattttg tccaggcggg ttatcagtat
     2521 ggttttaata atttattgac gctgtatggt ggctcgatgg tcgcgaataa ttattacgcg
     2581 tttactttgg ggactggctg gaatacacgc attggtgcca tttccgtcga tgccacgaag
     2641 tcgcatagta aacaagacaa cggcgatgtg tttgacgggc aaagttatca aattgcctac
     2701 aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg cggcctggcg ttattcatcg
     2761 cgtgattacc ggacatttaa tgatcacgtt tgggcaaaca ataaagataa ttatcgccgt
     2821 gatgaaaacg atgtctatga cattgccgat tattaccaga acgattttgg ccgtaaaaat
     2881 agcttctctg ccaatatgag ccagtcattg ccagaaggct gggggtctgt gtccttaagt
     2941 acgttatggc gagattactg ggggcgtagc ggcagcagta aggattatca gttgagttat
     3001 tccaataact ggcggcggat aagctatatc ctcgcggcaa gccaggcgta tgacgagaat
     3061 catcatgaag agaaacgttt taatattttt atatcgattc cctttgattg gggtgatgac
     3121 gttacgacgc ctcgtcggca aatatatatg tctaactcaa cgacgtttga tgatcagggg
     3181 tttgcctcaa ataatacggg attatcagga accgttggaa gccgggatca gtttaactat
     3241 ggggtcaacc tgagttatca gaatcaggga aatgaaacga cagctggggc gaatttgacc
     3301 tggaacgcgc cggttgcgac agtgaatggc agttatagtc agtcgagtac ttatcgacag
     3361 gctggagcca gtgtttcagg gggcattgtc gcctggtcgg ggggcgttaa tctggtgaac
     3421 cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc ttatgtcaat
     3481 gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg aatgacacct
     3541 tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg atagcgaagc agaattacgt
     3601 ggcaaccgga aaattgccgc cccttatcgc ggcgcggttg tactggttaa ttttgatacc
     3661 gatcagcgca agccctggtt tataaaagcg ttaagagcgg atgggcaacc attaatgttt
     3721 ggttatgaag tcaatgatat ccatggtcat aatattggcg ttgtcggtca gggaagccag
     3781 ttatttattc gcaccaatga agtaccgcca tcggttaatg tagcaattga taagcaacaa
     3841 ggactttcat gcacaatcac cttcggtaaa gagattgatg aaagtagaaa ctatatttgc
     3901 cagtaatagt caggtggtta tgaatattcg tataatgtta tttatagtaa tgatgatggt
     3961 tatgcctgtg agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt
     4021 tcagggggat tttgcactta ctcaaacaca aatggcgaca tatgagcata attttaatga
     4081 ttcgtcatgc gtgagtacaa atactatcac ccctatgagc ccgtcggata ttattgttgg
     4141 actttataac gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat
     4201 cacgttatca aataatcaga ccagtttcac cagtggttat tcggtaacgg tgacacctgc
     4261 ggccagtaat gcaaaagtga atgtttctgc ggggggcggc ggttcagtga tgattaatgg
     4321 agttgcgaca ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct
     4381 actgtgttta ttaggtggca agtcatggga tgcatgtgta aatagctaca gaaatgcatt
     4441 ggcacaaaat gcaggtgtct attcctttaa tctgacattg tcatacaacc cgataaccac
     4501 aacctgcaaa ccggacgatt tattaattac tttagacagt attcccgttt cacaattacc
     4561 agccacaggt aacaaagcaa caataaatag taaacaaggg gatattattc tgcgttgtaa
     4621 aaatttatta ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc
     4681 tgacttgtta accaacagca acacaatact gaaaggtgcg gaagataatg gcgtaggatt
     4741 tattcttgaa agtaatggtt cgtcagtcac acttttaaat atcactaaca gcagtaaagg
     4801 atacacaaat ttaaaagaaa ttgcggcaaa gtcaaaactt acagatacaa cggtttcaat
     4861 tccgataact gccagttact acgtctacga tacaaacaaa gttaaatctg gcgcactgga
     4921 ggcaaccgca ttaatcaacg tgaaatacga ctaataacaa gcaagacgag caagtggcta
     4981 ataataaaaa ataacaaggt gtctgcattt ccccctcatg atgagggggc tttttttagc
     5041 gatgataaaa aatctcaccg tcgtaggctt taatgatttt accgtcggtg tcgctgatca
     5101 acacgtacgc gccacccata taggtccagt gcgtcccggc atcaggcgcg ggcaggttac
     5161 gtaactgcca ctgcttaatg ttgtactcat cggtgcggta catctctggg acggtatcgc
     5221 ctggtttgaa atgcttaaag tcagcaatga aactgcttaa ctcatactta gaaattcccg
     5281 aggtattggt cgaacccgcg gtggctggtg cggcccaggc ggcactggta accagcaaaa
     5341 gcgcacccag caacatctta tttttaatgg tcataaactc tccactttgg ggatctgtaa
     5401 tatcagtcgc atatctttcc tgccatacaa tataactgac aagcgatatt ttgttagtga
     5461 tcccatgatt tcgtaaaaag tgtaaatgaa aggatgccct aaaagcatcc ttagcgagca
     5521 gagagtcagc ggaaatagct gatttaagaa tcatcctggg ctgaagataa catgcaagcc
     5581 taaaggattg agttatcgca ttatgcccat gaagccatgt acgcccatat acacaccgac
     5641 taagccaatc aacagactgg aaaaataggg ggcgcgttta gcgagagtgt taaatccgct
     5701 ccagcgtttt gcgacctgtt gaacgctgat tgctgcgcca acgcctacgg tgacaagcgt
     5761 taacgccagg ccaatgctga aactgacgac cagtgttgcg cccagtgtca gggctttcaa
     5821 ctgaatgcat atcaacagca cggtaattgc tgccgggcag gggataaggc caccggttaa
     5881 gccaaataac aaaatttgcc agttggtgac ctctctacca tcaaagcgtc gtttaatgtc
     5941 attggcatgg gctcgtgcat gggcatcctg atactcgcca tgttcgtgat ggtgatgatg
     6001 tccatggtcg tggtgatctt cgtgatcgtg gtgatgatgt tcatgatcat actcgtgcat
     6061 attctccagc cagttgcgtt cgccgcgcca ggtacgccag aacatccaga acgcggtgcc
     6121 aataatgatc actgcggaaa tcagctggag ccacggttct gctgattgag cagtaaagcg
     6181 cttgctgatc accatcccgc caaaggcaat taaccagacc actgcggtat gcgaaatagt
     6241 tgctgccagt ccgagcatca ccgcttgttt aatggtgcct ttgatggcga tgataaacgc
     6301 cgccatcatc gtttttgagt gccctggttc caggccatga agcgcaccaa gtaagatggc
     6361 gctggggatg aagaaccaag cgtttccttg ctgaagaaga gttgtaaatt cggtcatgat
     6421 aataattctt agtattaatt cggcaatctg attctactcc cccccagtac ctgatactac
     6481 cccccagtag attaatagtg ctatgatttt tcatattctt gttaaccagg tgttgccatg
     6541 tctcatacaa tccgtgataa acagaaactg aaagcgcgtg ccagtaagat tcagggccag
     6601 gtcgtggcgc tcaagaaaat gctcgacgag ccgcacgaat gcgctgcagt tttacaacag
     6661 attgctgcta tccgtggcgc ggtaaacggt ctgatgcggg aagtgattaa aggtcatctg
     6721 acggaacaca tcgttcacca gggggatgag ctaaaacgtg aagaagatct ggatgtcgtt
     6781 ctgaaggtgc tggattcata tatcaaataa tttattaacg cgattgtaaa actgccgttt
     6841 ttcctcgttt acaacgcgtg cgctggacat taccatcctc ctctgcgatt tatcatcgca
     6901 accaaacgac tcggggtgcc cttctgcgtg aaggctgaga aatacccgta tcacctgatc
     6961 tggataatgc cagcgtaggg aagtcacgga ccaccaggtc attgcttctt cacgttatgg
     7021 caggagcaaa ctatgcaagt cgacctgctg agttcagcgc aatctgcgca cgcgttacac
     7081 ctttttcacc aacattcccc tcttgtgcac tgcatgacca acgatgtggt gcaaaccttt
     7141 actgccaata ccttgctggc gctcggtgca tcgccagcga tggttatcga aaccgaagag
     7201 gccagtcagt ttgcggctat cgccagtgcc ttgttgatta acgttggcac actgacgcag
     7261 ccacgcgctc aggcgatgcg tgctgccgtt gagcaagcaa aaagctctca aacaccctgg
     7321 acgcttgatc cagtagcggt tggtgcgctc gattatcgcc gccatttttg tcatgaactt
     7381 ttatctttta aaccggcagc gatacgtggt aatgcttcgg aaatcatggc attagctggc
     7441 attgctaatg gtggacgggg agtggatacc actgacgccg cagctaacgc gatacccgct
     7501 gcacaaacac tggcacggga aactggcgca atcgtcgtgg tcactggcga ggtggattat
     7561 gttaccgatg gccatcgcgc cgttggcatt cacggtggcg atccgttaat gaccaaagtg
     7621 gtaggaactg gctgtgcatt atcggcggtt gtcgctgcct gctgtgcgtt accaggcgat
     7681 acgctggaaa atgtcgcatc tgcctgtcac tggatgaaac aagccggaga gcgcgcagtc
     7741 gccagaagcg aggggccagg cagttttgtt ccacatttcc ttgatgcgct ctggcaattg
     7801 acgcaggagg tgcaggcatg aaacgaatta acgctctgac gattgccggt actgatccca
     7861 gtggtggtgc ggggattcag gccgatctta aaaccttctc ggcacttggc gcttatggtt
     7921 gctcagttat tactgcactg gtggcgcaaa atacccgtgg cgtacagtcg gtgtatcgca
     7981 ttgagcctga ttttgtcgcc gcccagctcg attcggtgtt cagcgatgtg cgaatcgaca
     8041 ccactaaaat cggtatgttg gcggaaaccg atattgttga agcggtggca gaacggttgc
     8101 aacgttatca gatccaaaac gtggtactcg acaccgttat gctggcaaaa agcggcgacc
     8161 cgctgctttc accttcggcg gttgctacgc tgcgcagtcg attattgcca caggtttcat
     8221 taataacgcc aaacttgccc gaagctgccg ccttgctcga cgcgccacac gcgcgcaccg
     8281 aacaggaaat gctggaacaa gggcgatcgc tgttggcgat gggctgtggc gcagtgctaa
     8341 tgaaaggtgg tcatctggat gatgagcaaa gcccggactg gctgtttacc cgcgagggtg
     8401 aacaacggtt taccgcaccg cgcattatga ccaaaaacac ccacggcact ggttgtacgc
     8461 tctctgcggc gttggctgca ctacgcccgc gccatacaaa ctgggctgac accgtacagg
     8521 aggcaaaaag ctggctttca tcagcattag cccaggccga cacgctggaa gttggtcacg
     8581 gtattggtcc ggttcaccac ttccacgcct ggtggtgagc accgtactat agctgctttg
     8641 gttattgtca gcctttggca ctgacattca gggatgaaac caatgcagct aagaattacc
     8701 agtcgaaaaa aactcacctc tctattatgc gctcttgggc taatttccat cgttgcaatt
     8761 tatccgcgtc agacggtgaa ttttttctat tcgacagcag ttcagattac agactacatc
     8821 catttctacg gttatcgccc ggttaaatct ttcgctattc gcattcctgc cagttacacc
     8881 attcacggaa tagatgtttc tcgctggcag gagcggatcg actggcagcg ggtggcaaaa
     8941 atgcgcgaca acggtatccg cttacagttt gcttttatta aggcgacgga aggcgaaaag
     9001 ctggtggacc cgtatttttc gcgtaactgg caactaagcc gcgaaaatgg cctgctgcgc
     9061 ggggcgtatc attatttctc cccgtcggta gcagcgccgg ttcaggcacg attatttctg
     9121 caaacggtgg atttctcaca aggcgatttc cctgccgtgc tggacgtaga agaacgggga
     9181 aaattatcgg caaaagaatt acgcaagcgg gtaagtcagt ggctaaaaat ggtagaaaaa
     9241 cgcacgggaa aaaagccgat tatttactca ggagccgttt tttatcacac gaatctggcg
     9301 ggctatttta atgaatatcc gtggtgggtg gctcactatt atcaacgtcg tccggacaat
     9361 gacggaatgg cctggcgctt ctggcagcat tccgaccgtg gacaggtaga tggcatcaat
     9421 ggtccggtgg attttaatgt gtttaatggc acggtggagg agttgcaggg attcgttgat
     9481 gggattaaag aaacgcctta aataagaagc agtgatatac tcccaccaga ctccctggca
     9541 ggaaaaagac aaatggaaca agcgcatacc cagctgattg cccaactgaa cgagcgtatt
     9601 ttagcggcgg ataacacgcc gctttatatt aagtttgccg aaacggtaaa aaatgccgtg
     9661 cgcagcgggg tgctggagca tggcaatatt ttgcccggtg agcgtgattt aagtcagtta
     9721 accggcgttt cgcgcattac ggtgcgcaag gcgatgcagg cgctggaaga agagggtgtg
     9781 gtgacgcgtt cgcgcggtta cggcacgcag atcaacaaca tcttcgaata ctcgttgaaa
     9841 gaagcgcgag gtttttctca gcaggtggta ttgcgcggga aaaagcccga tacgctatgg
     9901 gttaacaagc gtgtcgtgaa atgtcccgaa gaagtcgcgc agcagctggc ggtcgaagca
     9961 ggaagtgatg tctttttgct taagcgtatt cgctatgtcg atgaagaagc ggtattgatt
    10021 gaggaatcgt gggttccggc gcatttaatt catgatgttg atgccatcgg gatttcactt
    10081 tatgactatt tccgcagcca gcacatttac ccacaacgta cgcgttcccg cgttagcgcc
    10141 cggatgccgg atgccgagtt tcagtcacat attcagttag atagcaaaat accggtgctg
    10201 gtgatcaagc aagtggcgct tgaccaacag caacggccta ttgagtacag cattagccac
    10261 tgtcgcagcg atctatacgt ttttgtgtgc gaggagtagt tcctcgcgcg ttggcgcaca
    10321 atcaccgccc cgatgcccga caacccacga cgccactgca ttgcccagta atacggcatc
    10381 cgccagtggc agaccagagg ccagcccggc aagtacgcca cctgcatgac tgtcgcccgc
    10441 cccaatggtg tctacaactt gcgttggaaa tgccggaatg cagccagaag cgtcgttgct
    10501 gaaataccat gcaccctctt tatcgagacg aacgatcaac ggcgcggcaa atttctcctg
    10561 ccattgcttg ccaagtgttg ttatctctgc ggataaagca aaacgttcgg cggcaatctc
    10621 agcctcttga cgattgagtg atactaaagg tcgacaggcc atgatccgcg ccagtaatgc
    10681 gtccgggata tcgccgatac gtgggccaaa atcgataaac ggcgtcacgt cttgcagctt
    10741 ttccagccat tccactaaca attcgccgca gggcgaggcc agttgataac cggaaaaata
    10801 gagcaggctg ccaggcgcaa cggttaatcg cgccagccac tggcgattcc actgattttc
    10861 cacaccgctg aatgacataa aagtgcgttc gccatccggc tcaaccagcg ccagacacca
    10921 gccgttatcg ccttcggcgt tatcgatcag gctgattaac ccctctttcg ccatccggtt
    10981 gcgaataatc tccgcccaca cgccctgacc gagcggcaag gcattacccg cttcgatgcc
    11041 gaggcgcttt aacgccacgg caatattcag tgcgcagccg ccaacgttaa cgctctgctg
    11101 cttcagttcg atatcgcacc cacgccaggg gagggcataa gcgtcggcga tcacatcaat
    11161 gaccgccgcg ccgacgacca tcaccggctg acgcgaggtt aattcaggca gcagcgtgtg
    11221 taatcgagcg ccgctcatac cgcctcccgt tgttgacgat atttcgccag cgctgtggca
    11281 tagcggttga aatcaagctg atttaccgca tccagttccg cctttaatgc aggatcgata
    11341 gcgttaacgc catgcaacgc gccgcaaatc gccgtcgcca tagcgccgat ggtgtctgtg
    11401 tcgccgccaa ggttagcgca caggacggcg cagcgattcg ggtcggtttg tgccagttca
    11461 accagcgcaa tggcgcacgg aacggactca atagtgctgg tacctgcgcc aacgacctgg
    11521 taaagctgtt cgctggcgga ttcggtgccg tcggcattgc gcacaatttt cagcgcaatc
    11581 tccagacgtg ctgccagtga ggcgctgaag gtagtgatgc gtttttgctg tgcatgtcgc
    11641 gcaattgaag gcagggaatc aacaatcgct gaccagcttt ctccgtcaat ggcacgagaa
    11701 atcgcccatg cgatgactac cgcacctgca actgccagat cggatttatg ggtcgggctc
    11761 gacgccagcg ccacatcatc aataaaggaa tcaacatcac gcgccggaag caaacaacct
    11821 aatggcgaga cgcgcatcgc tgcgccgttg gtcacgccgt tgttttccag ttcagcaacg
    11881 ggtttaccgt cgcgaatggc gttaagcgca atcttcgagg tcggacctag tacgtttttg
    11941 ttaaaggcgt cgaaacgcag cgcccagtcg agaatattac gcccaatcag atccggatcg
    12001 atcttgcctt cacgttccag taacgcatcc gccagacaca gcgccatcga ggtatcgtcg
    12061 gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc aggcagaaaa
    12121 cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc ggagggcatc
    12181 cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc tgttttcatt
    12241 taacttcccc ttgtgtcaac gcaatatcgc gatcatcgac cttgatagcc gtaatttcgt
    12301 tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc atcaccgcgc
    12361 cgaaagtcca catcccggac cagttgaaag tcagcccgtt taccggttcc ggataagcga
    12421 acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg aagccctggc
    12481 agcagagcgt gatcagcccc tgcgcggcgg tacgcatatg cacgggggct tttttatcga
    12541 catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc aaaataccga
    12601 ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag aagccatagc
    12661 ggatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag cgtttagtga
    12721 aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc atccagccgg
    12781 tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa tagaacgcca
    12841 gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta tcgcgcagca
    12901 ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg ctttttggcg
    12961 gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agaacttccg gcggtaatca
    13021 gcagcgggat gttagtcggt gagatatcgg cataccccag catttgcggc aagaaaccac
    13081 atgccagacc agaggcaatc cagccgatag tgcccatcac acgaatgcgc gggaaatcac
    13141 gctccacatc cggcacgttg gcaaaagcga tactgttagt cagcgcaatg gtcggcatat
    13201 aggttagaga gtaagccagc agtaacggaa agaacccggc aaaagtggtc tgttgcgcag
    13261 cgaaatacat cagcagcgcg cctgcgaaca tcaataccgc cagcactttt tgcgccgaga
    13321 aaaagcggtc agtgatggag ccaaccagaa ttggcgatag gatcgccgca atggcggtac
    13381 aggcatacga ccagccaatt tctccggcac taaaaccgct tttacttaac cacaaccaca
    13441 atggcacaaa ccacgcgccc cagataaacc attcaacaaa catcatgaac gacagctttg
    13501 ctgttgtttt catcttttaa ttccttcatg acaggtaatg taaggtacgc gatgacaata
    13561 ccacttaata atacctttta aatacctttg cggtgaaaac ttgaccgcgc taacattttt
    13621 tctgatgaat cgagccaaca gaaaacgctg aaaaaacatc caaaagatgg aaaaactcgc
    13681 ccgtaacgac caggattatg attgcccgca aaaggcgggc caggacaatc catagccgat
    13741 atccaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg cttggcaaag
    13801 acgccgacaa ccttttacag caccgttgta tgacaattcc ttctgaccag ctttatctcc
    13861 ccggacatga ctacgtagac cgcgtaatga ttgacaataa tcgcccgcca gcggtgttac
    13921 gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat ctttctattc
    13981 tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct aacccgctct
    14041 actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac tgtgtggcgt
    14101 caacttacgg cgtgctggcg tcggtatcgc ggcgttatgc gcatcgcatt ccattcctcg
    14161 tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa acgctgtatg
    14221 ccagcgtgga gcaggcgttc aacatgggcg cggttgcggt tggtgcgact atctattttg
    14281 gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcttttgaa cgtgcgcacg
    14341 agctgggtat ggtgacagtg ctgtgggcct atttgcgtaa ctccgccttt aagaaagatg
    14401 gcgttgatta ccatgtttcc gccgacctga ccggtcaggc aaaccatctg gcggcaacca
    14461 tcggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat aaagcaatta
    14521 attacggtta caccgacgat cgtgtttaca gcaaattgac cagcgaaaac ccgattgatc
    14581 tggtgcgtta tcagttagct aactgctata tgggtcgggc tgggttaata aactccggcg
    14641 gtgctgcggg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt atcaacaaac
    14701 gcgctggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg atggctgacg
    14761 gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt actattgcct
    14821 gatacgcctc atacgaacag caccaccccg taggacggat aaggcgttta cactgcatcc
    14881 ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag cactgccacg
    14941 tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt ccctatcggg
    15001 aggccgtttt tttgcctttc actcctcgaa caattttcat attgtcgttt ttgtgatcgt
    15061 tatctcgata tttaaaaaca aataatttca ttatattttg aaaacgaaaa caaacgacag
    15121 gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca gcgcggcggt
    15181 tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt ggtagaaacc
    15241 gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt tactcatgct
    15301 ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca ccatccactg
    15361 gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt tcgttctggc
    15421 gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat ttcacgggtc
    15481 aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaagcgga gctggggcaa
    15541 cttggcggcc aggaagatga tgtgcaagtc aatgaagccg atgcgttgta caccaacccc
    15601 gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt agccatcggc
    15661 acggctcatg gtatgtatgc cagcgcaccg gcgcttgatt tttctcgact ggagaacatt
    15721 cgccagtggg tgaacttacc gctggtgctg catggcgcgt cagggttatc gactaaggat
    15781 attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga gctgaaaaat
    15841 gccttctcgc aggcgttaaa aaattacctg accgagcacc ccgaagcgac cgatccccgg
    15901 gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt gattgccgat
    15961 tgtggctgcg agggcagggc ataacgcacc tgccatttaa taaggaaaaa acatgaaaac
    16021 gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct gttctgccca
    16081 tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca aagtgctgat
    16141 tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga caccggcaga
    16201 ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg aacgcattat
    16261 tctcggcggc gatcatctgg ggccaaactg ctggcagcaa gaaaatgcgg atgcggcgat
    16321 ggaaaaatcc gtcgagctgg taaaggaata tgttcgtgcc ggtttcagta aaattcatct
    16381 tgatgcgtca atgtcctgcg cgggggatcc cataccgtta gcaccagaaa cggttgcgga
    16441 acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc agcgtgagca
    16501 actgagctat gtcattggca ccgaagttcc ggttccgggc ggtgaggcca gcgccattca
    16561 gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc atcaaaaggc
    16621 ctttattgcc cgtgggctga cagaggcgtt aacacgcgtg attgccatcg tggtgcagcc
    16681 gggcgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag cgcagccgct
    16741 ggcgcaatgg atagaaagca cccgaatggt ttatgaagca cattctaccg attaccagac
    16801 ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag tcggtcccgc
    16861 attaaccttt gctttacgtg aggcgatatt tgcgctggcg caaattgagc aggaacttat
    16921 cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc tcgacgaacc
    16981 gcaatactgg aaaaatatta tcgtacgggt tttaacgatt cattactgga tattcgttac
    17041 agcctgtcgg atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgtcgaa
    17101 acgatgatgg tgaatcttga aggcgtggac atcccactgg gcatgattag tcagtatctt
    17161 cccaaacaat ttgaacgcat tcagtccggg gaattatcag caataccgca tcagctgatt
    17221 atggataaaa tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg
    17281 tatatgacta acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta
    17341 acccatatcg gtaatgagat gctcgccaaa ggtgtggttc atgatacctg gccacaggca
    17401 ttaattgcca gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgca
    17461 ataccgcatt gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca
    17521 aataaagttc attttcagca agcggatgat gataacgacg tggaggtatc gttggttatt
    17581 gcgttgattg ttgaaaaccc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag
    17641 ttacaacagc ccgatatcgt cgagacacta atcactcttc ctgaaactaa gttaaaggaa
    17701 tacttcacaa aatatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga
    17761 ctatttatga aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg
    17821 gcggcggaag aaattaaaga gttgtgtcag agtcataata ttcctgttga attaatccag
    17881 tgtcgggtta atgaaataga aacctatatg gatggtgtgc atttgatatg taccactgcc
    17941 agggtggatc gcagttttgg cgatattccg ttagttcacg gcatgccttt tatttctggt
    18001 gtcggtatcg aagcattaca aaataaaatt ctgactatct tacaggggtg acctatgttt
    18061 tcagaagtca tgcgttatat tctcgacctc ggccctacgg tgatgcttcc gattgtcatc
    18121 attatttttt ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat
    18181 atcgggattg gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt
    18241 ccggcggcga aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc
    18301 tggccgggct cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt
    18361 gcgattctgg ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat
    18421 atctggaata tctggcatat gaccttcacc ggcgcgttgc tgcatctggc aaccggttca
    18481 tggatgatag ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac
    18541 tggtttgccc gcgatacccg aaatttcttt gagctggaag gcattgctat tccgcacggt
    18601 acgtcggcgt atatggggcc gattgcggtg ctggtcgata ctatcatcga gaaaatccca
    18661 ggcgttaacc gaattaaatt tagcgccgac gatattcagc gtaaatttgg tccgtttggc
    18721 gagcctgtca ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat
    18781 gtcaaaggtg tattgcagct ggcggtaaaa acggcggcgg tgatgctgct gatgccacgg
    18841 gtgattaaac ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta
    18901 caggcgaagt tcggcggtca ggagttcctg attggccttg atccggcgtt gctgctggga
    18961 catacggcgg tggtatcggc aagcctgatt tttatcccac tcaccatttt aattgctgtt
    19021 tgtgtgccgg gtaatcaggt gctgccgttt ggcgatcttg ccaccatcgg cttcttcgtg
    19081 gcgatggcgg tcgccgtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc
    19141 attatgagca tcaccctgtg gatcgcgacg caaactattg gtttgcacac ccaactggcg
    19201 gctaatgctg gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct
    19261 cccattacct ggttactgat tcaggttttc tccccgcaaa atattcccgg tttcattatt
    19321 atcggtgcaa tttatctgac cggtattttc atgacctggc gtagagcgcg tggctttatt
    19381 aaacaagaga aagtcgttct cgcagaataa tttttacctg agggggaggt tatccccctt
    19441 aacaatcagg agtttttatg aaatcagtgg tgaatgatac tgatggtatc gtgcgcgttg
    19501 cagaaagcgt cattcctgaa attaaacatc aggatgaggt gcgggtaaaa attgccagct
    19561 cgggcttatg tggttccgat ttacccagga tatttaaaaa tggtgcacat tattatccaa
    19621 taacgttagg ccatgaattt agcggctata ttgatgcggt gggatccggt gttgatgatt
    19681 tacaccctga cgatgcggtt gcctgtgtgc cgttattacc ctgttttact tgtccagagt
    19741 gtctgaaagg gttttattcc cagtgcgcaa aatatgattt tattggctcg cggcgtgatg
    19801 gtggatttgc tgaatatatt gtcgttaagc gaaaaaatgt ctttgctcta cccacggata
    19861 tgcctattga ggatggggct tttattgagc cgattaccgt tggtctgcat gcttttcatt
    19921 tagcgcaagg ttgtgagaat aaaaacgtta ttattattgg tgccggaacc attggcctgc
    19981 tggctattca gtgcgctgtc gcgctgggag caaagagtgt gacggctatc gacattagct
    20041 cagaaaaact ggcactggca aaatctttcg gtgcgatgca aacatttaac agtagcgaaa
    20101 tgagcgcgcc gcaaatgcag agcgttttac gcgaactgcg ttttaatcag cttatcctcg
    20161 agacggctgg cgtaccgcaa actgtcgaac tggcggtaga gattgccggt cctcatgccc
    20221 aactggcgct ggtgggcacg ttgcatcagg atctgcattt aacatcggca acgtttggca
    20281 aaatattgcg taaagagctg acggttatcg gcagttggat gaactattcc agcccttggc
    20341 cggggcagga gtgggaaacg gcgagccggt tgctgacaga acgtaagtta agcctggagc
    20401 cattaatcgc tcaccgtgga agctttgaaa gcttcaccca ggcggtgcgt gacatcgctc
    20461 gtaatgccat gccgggcaaa gtgttgctca ttccatgaaa ccgcgggcca gcgtgatgct
    20521 ggcccggtat tgtgcaaaac agatcattca ccaatggtcc cccttcgttt acactagcca
    20581 caattgaata tgggtaaatg acgatgaatt cattcgagcg aaggaataag atcatccaat
    20641 tagtgaatga acagggaacc gtgcttgttc aggatctggc gggagtattt gctgcctcgg
    20701 aagcgacaat ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc
    20761 atggcggtgc ggcgaaaata atgtccggta atagtgaaac cgagacccag gaagtcgggt
    20821 ttaaagagcg atttcagctc gccagcgcgc caaaaaacag aatagcgcag gcggcagtca
    20881 aaatgatcca cgaagggatg accgttattc tcgacagcgg aagtacgacg atgcttatcg
    20941 ccgaaggatt aatgaccgcc aaaaatatca cggtgattac caacagtctc ccggcagcgt
    21001 ttgccctttc cgaaaataaa gacattactc tggtcgtctg cggtggcacg gtccgccata
    21061 aaacgcgctc gatgcatggt tccattgccg agcgttcatt gcaggatatt aatgccgatt
    21121 taatgtttgt cggtgctgat ggtattgatg cggttaatgg tattacgacc tttaatgaag
    21181 gttattcgat tagcggggcg atggtcaccg ccgctaataa agtgattgcc gttctcgatt
    21241 catcgaaatt taaccgtcgc ggttttaatc aggtactgcc aatcgaaaaa attgacatta
    21301 ttattaccga tgatgctgtg tctgaggtgg ataaactggc attgcagaag acacgggtaa
    21361 aattaattac ggtgtagtat tgttctgtat gccggataag gtatttacac tgcatccggc
    21421 atttcggatt ctccctggtt caagagagaa tagtttgatt aacgcaataa tggacaatct
    21481 ggtggtaatc gacaacgcaa cgccgccgga agtatttcaa tatgaaagtt ttgtccgctc
    21541 aacggttcgc catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac
    21601 gaagcgcctt cgataatatt cgggttatct tcgtcagatt ttaaggttga tacgagagcc
    21661 ggaagaattt catcgccggt aaaaatgcgc agttgcagca agccatcgtt aattaacgcg
    21721 ttcggacaca attgctgacc gccaccggcc tgacgcccgt taccaatacc aatgaccagg
    21781 gcgtcacctt gccagtgaaa gttttcaccg cggatttcac aacggtccgg ttgcagagta
    21841 tccatgcgca ttaagccatg aatgatgtaa gagacgccac ccagcgcggc ttttaatttt
    21901 tccggcgttt ctgtggtaat acgcgtccca aatccgcctg tcgccatatt aataaaacag
    21961 gtttgtttgt tgacctgcgc catatctatc gcaatggcgt taccggcaat tgccagtttc
    22021 agcgccttat ccagtgcctc aggaatccct acactggtgg caaaatcatt ggcggttcct
    22081 aatggcaaaa ttcccagcgc gggtatgtca tccccctcac actgaatcaa cgccgtagaa
    22141 acttcattaa tggtgccatc gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg
    22201 gcctcctcta catatcgtgc ggcatcgcct ttctcccagg tgacccgcac atggatcgtc
    22261 attccttcct cacgcaacag cataattgct tcgcgcaagg gtagattgtc agtacttttg
    22321 ccattaagaa tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt
    22381 ggtctgtaga tagtgtagag cagaaaacaa aaggtggagt cagaacagga tgaaagtcgg
    22441 aggataaaaa gtcagttcaa gtatggagga ttcagtggtt accaccagga gcttgcaatg
    22501 gggacagcga atactaacgg ctgccaccga taaatttcaa aaaagagcat atgcctaata
    22561 ttcaaccaaa cagtggcatc ttaaatatac tattccaaat atattaaagc ccccatggag
    22621 ttaccctgaa gggcctcaat gtccgtaatt cctacttatg taggaaatgt tgtacagaac
    22681 atttattata atcctattca attataa
//
LOCUS       DAZFFI010000073        22566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFFI010000073 DAZFFI010000000
VERSION     DAZFFI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.073"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..1589)
                     /gene="amn"
                     /locus_tag="ACHJ12_003263"
     CDS             complement(135..1589)
                     /gene="amn"
                     /locus_tag="ACHJ12_003263"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4991007.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTLAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1691..3007)
                     /gene="shiA"
                     /locus_tag="ACHJ12_003264"
     CDS             complement(1691..3007)
                     /gene="shiA"
                     /locus_tag="ACHJ12_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4991008.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            3322..4374
                     /locus_tag="ACHJ12_003265"
     CDS             3322..4374
                     /locus_tag="ACHJ12_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4991009.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4636..11713)
                     /gene="yeeJ"
                     /locus_tag="ACHJ12_003266"
                     /pseudo
     CDS             complement(4636..11713)
                     /gene="yeeJ"
                     /locus_tag="ACHJ12_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(12026..12101)
                     /locus_tag="ACHJ12_003267"
     tRNA            complement(12026..12101)
                     /locus_tag="ACHJ12_003267"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12066..12068),aa:Asn,seq:gtt)
     gene            complement(12202..12999)
                     /gene="mtfA"
                     /locus_tag="ACHJ12_003268"
     CDS             complement(12202..12999)
                     /gene="mtfA"
                     /locus_tag="ACHJ12_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4991010.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            13093..13182
                     /locus_tag="ACHJ12_003269"
     tRNA            13093..13182
                     /locus_tag="ACHJ12_003269"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13127..13129,aa:Ser,seq:cga)
     gene            complement(13752..14282)
                     /gene="yodB"
                     /locus_tag="ACHJ12_003270"
     CDS             complement(13752..14282)
                     /gene="yodB"
                     /locus_tag="ACHJ12_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4991011.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(14625..15275)
                     /gene="zinT"
                     /locus_tag="ACHJ12_003271"
     CDS             complement(14625..15275)
                     /gene="zinT"
                     /locus_tag="ACHJ12_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO4991012.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRKLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(15528..16163)
                     /gene="msrQ"
                     /locus_tag="ACHJ12_003272"
     CDS             complement(15528..16163)
                     /gene="msrQ"
                     /locus_tag="ACHJ12_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4991013.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(16164..17168)
                     /gene="msrP"
                     /locus_tag="ACHJ12_003273"
     CDS             complement(16164..17168)
                     /gene="msrP"
                     /locus_tag="ACHJ12_003273"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4991014.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(17277..17690)
                     /gene="hiuH"
                     /locus_tag="ACHJ12_003274"
     CDS             complement(17277..17690)
                     /gene="hiuH"
                     /locus_tag="ACHJ12_003274"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4991015.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            17823..18494
                     /gene="hprR"
                     /locus_tag="ACHJ12_003275"
     CDS             17823..18494
                     /gene="hprR"
                     /locus_tag="ACHJ12_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4991016.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSSLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            18494..19852
                     /gene="hprS"
                     /locus_tag="ACHJ12_003276"
     CDS             18494..19852
                     /gene="hprS"
                     /locus_tag="ACHJ12_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4991017.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(19960..20811)
                     /gene="hchA"
                     /locus_tag="ACHJ12_003277"
     CDS             complement(19960..20811)
                     /gene="hchA"
                     /locus_tag="ACHJ12_003277"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4991018.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(21403..22476)
                     /locus_tag="ACHJ12_003278"
     CDS             complement(21403..22476)
                     /locus_tag="ACHJ12_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4991019.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         5953 a         5082 c         5335 g         6196 t
ORIGIN      
        1 gtggaaatca ttaagacgat aataaaacac gtatccgccc aaggaagcaa acagcccacg
       61 attattgcag gctatttgcc aggtgtctta cttactcctt cacatctatt tctgataaga
      121 aggttcagaa cttattatcg gaacggcggc tcattaaagg ttcgtaattt acgcgaatgc
      181 agtcggtcgc cttctgcgcg cagcaaatcg atcgcccgaa tgccaatttg cagatgttcg
      241 gaaatagcgc cttcataaaa acggttagcc tggccgggaa gtttaatctc gccatgcaac
      301 ggtttatctg aaacacacag tagtgtcccg tatggcacgc ggaaacgata tccttgcgcg
      361 gcaatggttg cactttccat atcaattgct acggcccggc ttaagttaaa acgaagtgca
      421 gaagctgagt aacgtaattc ccagttcctg tcatctgtgg ttaccacagt accagtacgt
      481 agccgctgtt tgacttcctc accgggcctg ccgctcacca gcttggtggc gtcataaagc
      541 gcacgttgca cttcagcaat gctcggaata ggaatatcgg gcggcagaac cgcatcaaga
      601 acgtggtcat cgcgtaaata agcgtgtgca agtacataat cgccaatggc ctgactttca
      661 cgtaatccgc cacagtgacc aatcatcaac cagacatccg ggcgtagcac tgccagatga
      721 tcgcagatgg ttttagcatt tgacggtccc acgccaatat tcaccagagt aataccctga
      781 ccatcggcgg taattaaatg ccatgctggc atctgatgtt ttttccatgc aaggtcggaa
      841 atggcttctt ctggcgcttc ggtttcggcg gtgatccagt tcccgccagc acaagaaagg
      901 gcaatgtagg gactatcagg atcgaggatc tggctgcatc cccaacgaac gaattcatcc
      961 acataacgtg tgtagttggt aaacaagacg aacggctgaa aatgttcaac tggcgtaccg
     1021 gtataatggc gcaaccgcgc gagggaaaaa tcgacgcggc gcgcatcaaa atgcgatagc
     1081 ggggagaatt cagttggatg ataaatgccg tctgcagttt catcgccaat ttgcgccagt
     1141 tctgttgtcg ggaagtagcg agttaaccca gcgctcattg agcgatcaag tgtcaattca
     1201 gagccatcga tgacataagg ataggggatt tcatgctgcg agggttgcac tgagatatgc
     1261 gcaccataat cctgatacag caacgtaagt tgttcattaa gatacgaacg aaagagagta
     1321 gggcgagtaa tcgtggtggt gtagctgcct gcgtgggtaa agcgaccaaa tgcgagcgtt
     1381 ttggggggat tggttgtgct accgtcccag gttacggtca gtgatggata gacaaaaaga
     1441 ccttgtttgc gggcgttttc atcaggtaat tcgccacttg taatatagtt gccaatggcg
     1501 ttgcgtaatg cgactacaga ttgctcatac agcgcgtcga gtttatccag tgcctgagct
     1561 ggggtcagac cggagccctt attattcatg tgtgtctcct gttccataca attaccgcac
     1621 atccgatagt atgtcacaga aatgtgaaac aaaagtgtaa ggcgaagatc ggatgatctt
     1681 cgccaggcta tcaagcgcgt tgactgtctt tcatcaacaa agcggtcatt gcggaaatca
     1741 ggcatccagc cagcaaataa atggcgacgc tatgccagtt cccggcaaag taagtgatga
     1801 gtgcagcggc aataaaaggt gtaaatccac cgccaaccac actggcaacc tgataaccga
     1861 ctccagcgcc actatagcga taactggcac caaacatttc ggtaaacatc ggttgttgca
     1921 cacacaccac catgtcatgc gcaatgtttg ccagcattat ggagaagaaa actatccaga
     1981 aaatggattg tgcttcaagc gccataaaga aaggaaatgc gctcaacgtt ccgattaacg
     2041 cgcctgtgat ataaaccctg cggcgaccaa aacgatcggc aagccaggca aaacagggaa
     2101 ttgtcaggca gcttaatcca cctaccagca aaccaatatt gaggaaaagt tcgcgcggta
     2161 accccatatt ctgggttgaa taattaagtg cgaaggcggt aacgatgtac atcgtcagca
     2221 actcgcacag tcgtagcgca ataatcttca ggaaagcacc aggatgccgt aatagtgctt
     2281 cgataaccgg aatgcgtttt ttcgcggccg cttgattatg ttgctgttgt tcaaattccg
     2341 cggactcctc catgccatta cgcacccaca atgctcccag taccagtacg atgctaaaca
     2401 ggaaaggaat gcgccagccc cagcttaaaa actgttcgtc agtcgtcatc atactgatca
     2461 atgaaaccag tccagttgaa agcagtaaac ctacaccgta gccaacttgt acaccgctac
     2521 tgtaaaaggc ttttttattt tttggtgcac tttcaacgga aagcaacgcc gcgcctcccc
     2581 attcgccgcc gacggcaaat ccctgaatgg cacgcagcgt caccagtaaa ataggtgccc
     2641 accacccaat ggtcgagaat gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca
     2701 tccagacggt cagcattaac atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa
     2761 tgacaccgcc gagcggacgg aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg
     2821 ttcccatcgc cgggcttact tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga
     2881 tgccatagag taaaaaatca taccagtcga cgacggcacc agcgaagctg cctaacgcag
     2941 ctcgtcgggc gcgacttaac gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg
     3001 agtccataat gatcctgttt gtactgtctt tttataatta gtatggaaaa ggtaactcac
     3061 attgccgtcg atctataaac gaaccttatg agactacctc gacagagcgc atctgcaaat
     3121 gattttatgg ctgattagcg gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt
     3181 tgctggtgtt gtttaaggat taagcagagg acccacaggt tagagatatg aaacataaaa
     3241 gtccgcatat gcacaaaatg gcaccctgat gagtctgata gactgcattg cattataacc
     3301 agtagggagg cgaggttagg tatgttttta gcatcattgt tgagacgtat tgcatttagt
     3361 tactacgatt ataaagctta taatttcaat attgaaaaaa cagactttgt tgtcatccat
     3421 attcccgacc agattggcga tgctatggcc atctttcctg ttattcgggc gcttgaattg
     3481 cataaaatta agcatctttt aattgttacg tcgacaatta atttagaagt gtttaatgca
     3541 cttaaacttg aaaagactaa attaacatta gtcacaatga ctatgcagga tcacgcaaca
     3601 ttaaaagaaa taaaagattt agcaaagaac ataacacagc aatacggtac gccggatctt
     3661 tgtatagagg cgatgcgtaa aaagaacctg aaaacgatga tatttatcag tcagttgaaa
     3721 gcaaaaacga attttcaggt tgttggttta accatgaagt gctactcccc tttgtgcaag
     3781 aacgcgtccc ggatggatca gaatctccgg gctcctgtac ccatgacatg ggcatttatg
     3841 atgcgtgagg cgggttttcc agcagtcagg ccaatatatg aattgccact aagtgaggat
     3901 gtactcgatg aggtgcgcga ggaaatgcga tcgttaggat cttacattgc gcttaattta
     3961 gaaggtagct cgcaggaacg tacattttca ttatcgattg cagaaaatct aatagcaaaa
     4021 attcaaagtg aaacagatat accaatagtg atcgttcatg gaccaaaagg ggaagataaa
     4081 gccagggtat tagtggattg ttataataat gtctaccgtt tatccttatc accctcgatt
     4141 aaacgttcag cagcaatcat aaaagatgct tatatcgcaa taactcctga cacctcaata
     4201 ttacatatgg caagtgccta taatactccc gttgttgcaa tttatgctga ttacaaaacg
     4261 cgatggcccg caatggccga tgtttcggag tcagtcgtcg ttgggcaaaa aattgacaat
     4321 ataagtctgg atgaattcgc aaaggcatta aaaagtgttt tggcgagaat atgagacagt
     4381 acttatgcat agtaatgccg atctgttatg aacggtggtg tcatcaatac tctgaggctg
     4441 gtcaatcatc acagaaaacc agcctttgga tcaaagctgt actcaccgga tacgatcata
     4501 gtcacatcga acgcgattct tgcggtgcag cagttttatt tattcatttt ctgtgagaga
     4561 agggcaagct ctatagaggg aatgtaaaaa agagtgctcc gaatggagca ctctttaaga
     4621 aattataacg gaaaatcaca ggtttttata acatgtcgca taagcaaacc caagcttttc
     4681 aaatacgctt tgatcacctg ttgccaggga aactacgtat tgttcgcttg agtttgcggg
     4741 actagatgac caatacatat ttgattgaaa accagcttca gtcgtgtaat gccccatatc
     4801 tccccattca ctgtacagtg acccgatgcc tcgggaacga ctatggttta aatggtctgc
     4861 acggggaagt gtgtagccat tgctactgca aaaattttca gcaaggctgt atatcatgaa
     4921 agcatcgccg gagttcaccc accaactctt cacacggatt tcgtatacat agctagggcc
     4981 tcctgatttt ggcgtcgccg taatcctttc ccaattgctg ccgacatttt taaatgtcac
     5041 tttaccggta gcatcaacat cgacccagga ggcagagctt gaaaactcat aatcagccgc
     5101 cgtttttcct ggggcaaagt tgtcattatt caactgataa tacgccccgg tgaacccctg
     5161 cgaagggaat gtagttgtcg gtaggtcagt accattgact gatactgtac cgctcattat
     5221 tttgtcttct gcgcgagtga attgtattgt ggtactcaga ccagcttgat gaacaccatt
     5281 caatacaggg atcagcgttg cgataccctc tccgccgcct gtaatggtgg ctttgtaatc
     5341 accgttgata ttttgactgt aatcaattgc gctaattttc atatagggca cgttagtacc
     5401 tgattgcaca aattccatcc cttcagaaac tttgatcgga ttgcctgata tatcgtgcaa
     5461 aacaagatgt agctcagcac tatcggtaaa gtcccctttc agtgaggacc gattgttctt
     5521 aaggacggac tgcgaggcgt ctgccgggcc tgccaccagc gttatatcta ctgtttgcat
     5581 tccaccagcg gtcgtgactg cgcttaccgt gacgctacct gtcatcgctc ctttcacgct
     5641 tgttgtcgcg attccgtttt gatcggtcac cgctgttaat gacgttaatg tggcagagcc
     5701 gctttttaag gcgaagtaca cagtgagacc ttcgatcagg ttaccactgc catcctcaac
     5761 cgttgccttt aaggtactta aatcactgtt ggtggcggcg atagtcgatg gatcagcgat
     5821 aaagctagca acatgtgcgg tgcttgagtt gccagtgact ttcacggttg tctgtgtctg
     5881 tattgttacg ccgttatgga acgatgcagt caccgtatat gtaccgactt tattgctggt
     5941 cagaacgacc ggagcctgac ctgaagagtt agttctcact tttccgccac ttaacgtcgc
     6001 cccttctggc gttacgctaa agttgatgac ctgaccctcc actggagtgc catttgcaga
     6061 ggttagcgtt gccgtcagag tagtgcctgt aggggcatag acaccgataa gcggactgga
     6121 cgccgtgagt gtcagttttt catcaatagc ctccagttgt ttctcaagtg aggctccatt
     6181 cgccagggat gctttcacca tatacgaacc gtttctttcg ggcgtcatgg agacctcggc
     6241 tataccatgc gtatcagtag agacaatatt ttggctgatg gtcatgtgct ctggtggctc
     6301 tgcactgaat gttacagatt cattaagaat cgggttgcca aactggtcgt tgacgtgagc
     6361 tttaactgct acgtcttgtg cgaccattag ctgaccttca ggtatctcca gactggtaat
     6421 cgttgcagaa ttaatatctg cttttgcatt cagtaatcgc gatatgactt cagtaggttt
     6481 atctgccaga gaggctgaaa ctgttttcag tccgacttcg gtgcttgtca caagaatttc
     6541 agcgaaaccc tgatcatccg tttcaacgct ggtgctgctt agcgtgacgg aggttccgcg
     6601 gaaattaact tttatgcctt ctaccgggtt gccgttagcg tcagtgacgc ttgcagtcat
     6661 ggtcgcgccc tcggtcgtgc tgacgacgaa tgagtataca gaggttaagg aggctagtgt
     6721 tgcggtacca gcatcggcaa tcaaagtcac ctgtttcgta tcactgacac cataattgat
     6781 cacgctaact gtcacgggat aggtgccgga tttggtaccg ctcagtgtaa cttcagcctt
     6841 gccgttgata tcagtaatgg cggaaccgcc ttgtccgaga gtgaagcttg cgctcacatc
     6901 tgccggtagc gtgaatgtca ccgcctcatt ggcgaatggg ttgccgtttc catcagtcac
     6961 tgttgcctgc agcttggtca tcccaatgtt attggcgatg aaattgtcct cggtaacgtt
     7021 aagattaacc tgcgcagtga gcgtatccgc agtaaagttc accaccagtt gtccactctt
     7081 actgccagcc atcgatgctg taacagtatg agcgcctgct tttatacctt tcagcgtgac
     7141 tttcgctttg ccttcagtat cagtaatcgc tttaccgcca tcgctcagcg tgaagttcgc
     7201 cctcacatct tccggcagag taaatgttac ctcagtgttg gctatcgcat tgccctctgt
     7261 atcagcgact gttgctgtta gtgttgtgag atcgttattg tcggcaatca ccggatcctt
     7321 cgaggctgcc agcgtgattt cagcattcgc gacgttcggc acataggtca ctgtttgttg
     7381 catcgaatcg ccattgtcga gggttgccgt cacttgataa accccggctt ttgtagcggt
     7441 aaagcttgcg taaaaatagc cgtagtagtt ggtagtatat ataccgttat tactgagggt
     7501 cacgccttca cttggtgaaa cgctgagggt tacctgatgc tggggtacac cattgccgta
     7561 attatccttc acctcaatat acagactggt tgtctcgcct gcggagactg tatcaaaaag
     7621 tgcatgtagc aaggtgagat gtgctgtaga cgcatccgcg ttgacattaa ttgatgtgct
     7681 aagcgtggag ttaccatttt ccagactggc ctcaacggta tccggtctcg ctcctgattc
     7741 tatcgaggag cgggtattgg tataagtgac ggttgcctta ccctgttcgt tcgttacggc
     7801 ttggccgcca ttcgtcattt cggcagagtt tgttcggctg gtgaagttca cagtcacacc
     7861 tttcaccgga aagccattat tatcaacgac tgtggcggtg atgacgctgc cggtgctgtt
     7921 ctgcggggta ccggcgatta tgctgtctgg gacaggcgtc aactcgataa tttttgccgc
     7981 cgctgtgtcg ccaataaaat gcacagtttt gttgtcgttg gcaccattat tagccagtga
     8041 tgccgtgacc gtctgctcac caaaggcaac gcctgcgata gtggcctgcg cgatgccaga
     8101 ctcattggta ttactcactc ccggggtcag ggtgagtcct gaagaggctg agctgaatgt
     8161 taccggaaga ttattcacct cattgtcgaa ctgatcttta accgttgcag ttagcgttgc
     8221 gctatcgacg ccattacctg tgatctcatc ttttgatatc tgcaggacaa cctgagccga
     8281 ggctttgtcc gccacaaatg ttaccggctg cgaatcactg gtattgttat tacccagcgt
     8341 tgcggtaacc gtatgcgtgc ccgctttttt acctttcagc gtgacatgcg cttccccatt
     8401 ggcctgagtg atggcaatac cgttattttc aagggtaaag tttgccgcaa cgtcctgtgg
     8461 catggtgaag ttgaccgtta tccccgccac cggatgattc gacggatctt tcactgttgc
     8521 tgtcagagtt gtctcatcca cgccattccc aatgatttcc gctttcgatg tttgcagaac
     8581 gacaaccgct ctgtctttat ccgccacaaa cgtcattggc tgtgtatcgc tgacattgct
     8641 gttagccaga cgagccgtga tcatatgcgt gcccgctaat gtgccggtca gggaggcgat
     8701 tgctatcccg ttgctatccg tcatgccttt tccgctgttg ctaatcgaga accgacttgc
     8761 gacgtcgttt ggcacagaga aggtgatttc tttatctttt agtgggttgc catttttatc
     8821 ctgcaaggtt gcagtcatat gtagcggagc tgtgttggtg acggtgatat cacctgaagg
     8881 cacactgagg gtcagggcag cagtactttg atcaccgata aaaatcacct gttgattagc
     8941 ctgagaacca gagctcacag aggccgtaac cgtataatca ccatttttca aactggtcag
     9001 cgtagctgtg gcgatcccgt cgtggctatt cacttcggtt tggctcagtt tcgctgctgc
     9061 tgaattaaca ttgaaggtga ccttgacgtc attgagcagg ttgccttttg catcccggac
     9121 tgtcgcggtc attgtggcgc tgtcattgcc gtcagcgacc acttcatttt tcgacttctg
     9181 cagatcaacc tgcgcggtac ttgagtcgcc gacaaaactg acgattaacg tttgtttcac
     9241 gccattttca agggtgactt caaccgtgtt gtcttcctgc ttactacttt tcagatcaaa
     9301 agtcgccaga ccattaacat ccgtttttgc ggtgttttga ttgttgaagg aagttgccga
     9361 tccgcttaat accgcaaacg tgacggtatg atcattgatt gggttgcttc cttcatcagc
     9421 gacattgacc gagacggtgt ttgctgcatt ctcattggcg agcacaccat tattgctggc
     9481 agataatgtc gcaattttcg ctgactgcgg gttggcgtcg atgataaatc cagcggtatg
     9541 taaatcttca ttccagtttt gcattaatag cttcgcagta agcccactgc ctttggtata
     9601 ggcggtatag gttgccttat agacgccatc tgcggtttct ttccagtctg tagtgacacc
     9661 aggtttcacg ttgtcgatgc tgactgcggt attcagttgc tgtttttgct ccttaacagg
     9721 tttgtcattt tcatctctca gttctaccgt tacctcgata ggattcccgg agagataacg
     9781 gtccttatca atcttaattg acgagtgagt ccgggatgac gaaacagaaa tgatattcac
     9841 cacggcgggg gctttagcct catccacacc gttcagctgt ggcataagcg tcagcgtgcc
     9901 agacagcgct cctgtggtca ggacttgggt atagcttccg tcaccattat ctttccagtc
     9961 agacagggtg atgtcctgaa ctccttcgtg acgcgtcgaa agcaccagcc cgataacagg
    10021 attacctgct gcatcatgag caataaaagt cagtgtggcg gttgaatggg aatccgcgct
    10081 caatgtttgg gtacttaacg ataccgagga atctttctgg cttagcgtag gtgcctgaac
    10141 gaccaccatg ctttgttcac gattcgacaa attgcctttg acatcttcag cggtgacttc
    10201 aatcggccag gtgttatcgg tttctggcgt actggtgaac cggtaacccg gcagggtaac
    10261 cagaatatct ttacccgttg tgaccacttt gccaccggca gcttccagtg cggtggcttc
    10321 gacgttatag cctttcaggg catatttggt ttgtagcgac gaaaccagtg atttcacttc
    10381 tcctgacttc cctgtcacgg ggtctgtcag ggtcaggcgg accagttctt ttttgcgata
    10441 ttccagaacg atgttgttgt tgcgatccac cagatcataa cggctgcctg caaggctacg
    10501 ccgtgcagcg acttcattcg ggtcaagctg tttctgcatt gcgctgccag gttgccaggt
    10561 aaaatcgacg gcaaaacggg tgtcattttc gccctgttta ccctggcgtt gctccgcgct
    10621 gaaggtcatt agcgggaagg gggtatagtt aagtccagcg gttatggcat gaggattact
    10681 ttgccgatcg tctttatcga acagggccac ttcatcgcca taatactgtt catagaccag
    10741 tttaccgcca aggtgcggcc aggcgggtag ccagccttct gcgcgtacat cccagccatt
    10801 ggccgggcgt gcttcataat cgttgtccag ttcaggtgcg ctgcgccagt tggtcagtcg
    10861 caaatagccg ttactgctta attttagata gtcgcgccag tactccgcgc caatgccggc
    10921 gcgggagtgg taacggctaa gatcgtggtc gaaaaagaag ttgatgcccg acatccatgt
    10981 gggagtgaaa tgacgccaac ctaagccgtt gttaatctgt gtacgctcgt cagtacgatg
    11041 gagagtatgc tgactgaaaa agagattatc aggcgtttca taccacggat ggagaaaatc
    11101 gaactgggag ttcttcaggc taaaatcttc atccacgccc agcgtgattc ttgcggtacc
    11161 gaagcggctt aaccagtctg tcattgcgcc tgaagcctga gaagaggccc atccacgcgc
    11221 catatttgcc gcttgctcgc tgttcatatc ctcggcgagc agagacccga tttgctgtga
    11281 agtactggct atctgttgct caaggttgcc gctgctatta cccggtggcg gggttaaatt
    11341 attttcacta acttgtgccg ggacatccag ttcatcaccc tggcggacat tatcaaaacc
    11401 tcgagcaaac gtacgaaact ggttgagttt gcgtaactca gccaccgaaa tgccgaaacg
    11461 ttcggcaacg ctttgggccg atttccagcg ctccaagggt gtagggcacc gtattggcat
    11521 ttgcaatggc gagttgtgca ggaactggtt gttgggttgc ggcgtttacc acaccttgtg
    11581 ctgccgcagc catagggaac gcaagttgag ttatcaggca gatacccgca gttaagcggc
    11641 gtagtttaat tcccatcccg cataagattt gtcggtcatt tatttcttct ccacttctct
    11701 tcttcgtagc catgcttacc ttccctgaac gagctaacat aaatgcgccc tttctagcac
    11761 agcgaaaaaa tgtaaatcct gatagcaata tgattgaaaa ttaataactt ttcgttaggc
    11821 agttttgggt gtgagttgca agaggggaga ctactgaata actcaagttt tataatcgag
    11881 gggaaaatgg tgatggcgtt catagcaaaa cgccctcaac cataaaggtc gagggcgctt
    11941 aagatgttaa aaacccgcta tccgttaata aacaatgttc aactaaggtt agtgacattg
    12001 cgctaagaag cgaattttta gaatttggct cctctgactg gactcgaacc agtgacatac
    12061 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga acggggcgaa
    12121 tattacttag cggtaccttg tctgtcaaca ctaaatttca taagtcattt caattggtta
    12181 attaatctgc aaagttgtta attaatgaac attcgtcgcc gaaaacgagt ctgtatcatt
    12241 agtgcgatgc agtctctgca aaggatcttg ttgataaaat tggcagaaac gttgccacaa
    12301 tgaagggaaa cgaggagcaa aaagttctgg ggcgctaaag aaatattcag aaagtacggc
    12361 aaaacattca gcaggatcac tggcagcata agcatcaatg ctcgccgcat tctcgccaac
    12421 taattcgatt tcttcctgaa tgttgttcat tgcagcatga agatcgtgtt cccagccagc
    12481 aacctcacgc aacggaataa agggaactcc gctggcgcga tcgccgttac gggtgtccag
    12541 cttatgagcg acttcatgaa taatcaggtt aaaaccagaa gcatcaaaag aatcttgtat
    12601 atccaaccag ttcaaaacga taggcccttg ctgccagctc tgacctgact gaacaatacg
    12661 ttggttatgc accagaccga tatcgtcttc ccattcatca tcgaccacaa atggcgcagg
    12721 ataaattaag acttcatgaa aaccatccag ccattccagt cctaactcca gaacgggtag
    12781 gcaaaataga agtgctatcc ggcagcttct taatgaatcc agttcaaagc cctgtaaagg
    12841 aacaagccgc ttttgctgta aaaaacgttc ggcaagagtg actaatttgc tttgttcctg
    12901 ttccgtcaga cacgttaaaa gggggatcga tagtgcttcc tgccagggaa gggcagtttg
    12961 atgtgctgat tcttgtactt tccagggcca cttaatcatc gttttgctcg caaactcgtc
    13021 acttgaacaa aattgcacgg acagggactg ttaaaatgcc aaatttcctg gcatcatggc
    13081 aaccatctga acggagagat gccggagcgg ctgaacggac cggtctcgaa aaccggagta
    13141 ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactttatca atgacttatc
    13201 tcccgacttc ccgccttgct tttcctaaac agaacaatcg tagaatattc ttgaagggtt
    13261 agatcgtcac tgttttctgt tcgatactgt gacattcagc acttgattcg ctatggatct
    13321 gacaggaagc tttcgagcga aaatgtgcag ttattcagtc gttttcttat cgctcaccat
    13381 tattctttta gacattcatc ctatgaaact gccgcaaagt tggtggtggg aactgaagtt
    13441 gcgtagagaa ggggtcaata attgagtcaa aaaaggaacg cggccgtcag attaaagccg
    13501 cgggacaaag tccataattt ccaagagttg ttgaatttcc cagctttttt gaaagccttt
    13561 gtatcatact aacaagtaaa aaaggtttaa ataccaaaat taacctgtat ttttgataag
    13621 tcttaccatt gtacgattta tacagtttct gctcctcctt gttgatagtg gaaagggtgc
    13681 attgtgcact tgcgtaaaca tttatgacgc gttgatgtcc atggttcaca tttccttgtc
    13741 aggggatcga cttatttgcg gcgaggcatc attcgtaaca gagtattatc tttctgaatg
    13801 tagtgatgaa agagtgctgc gccagcgtga gctgcgatta aaaagtaacc tatatttgcc
    13861 caggtttcgt gaatattttt tatcagtgct ttaatttcgc tgtttggggt aacaaaggga
    13921 gacacattga aaccaaggaa actccacgat tttccactgt aagccatcaa agcaatcccc
    13981 agcagaggaa gtgcaaggaa ggttatataa agcatgatgt gcattagcga agccgctttc
    14041 atttgccagg caggtggcgg tggcacaata gaaggatcac tataacggtg ttttataatc
    14101 aggcgtgaaa acattaacac ccaaacgaat atacccgcat tgtaatgtgt ttctcgcatc
    14161 agcagataag tactactacc tttaggaaac cagccacgga gttccatcgc ggcataggtt
    14221 attgcaacga aaagcagcgt tatccaatgt aaataaattt gagtttttga gaatcggttc
    14281 atttttgtag ctcctgtaat ttttacagtc aagctatccc acgaacctta aaaaatcctt
    14341 atacagatgt ggttttaaga atttgttatt taaaaaattg atagtgcaca ttgtaaccag
    14401 ccaatataag caatggcgtg agcacaggta tctttctctg ttggccgtat tgtgtatgga
    14461 atccgttatt ggttaactca atgcgggtta gcttattaat atacagacac gtcatagtga
    14521 tgagcgtgtc tgtagctagc ttgcgttcag tggcgtttgt ggatggacca gtggcagtta
    14581 tgatgactta ctaaagcggc atcgaggcgt tatcatgaga atactcaatg agacatcatt
    14641 tcctcgacca cttcttcgct actcaactga tatggataat acgttggcca gttttccatt
    14701 tcatttaata aagattgctg tgaatcatta cccataaaaa tgtgaaaatg agatgatttt
    14761 cgtggtgcaa ttatatgatc actaaattgt atatatttag gggctttgct ttcaggatct
    14821 ttacattcga ataagtaacg aacgcctttc ttgcctgatt tataggtgag tattttgtat
    14881 ccatcgtaat catatttaca ggatgttgtt tcattatttc tatggaattc aacaatgccg
    14941 tcctcaatgc caatcatctc gatatctgtt gcataacctt tgtgataata atctttaatt
    15001 tcagcaaatg ttttagtttt atctgcatcc gctttcttct gaaatacagg gtcaagtttg
    15061 ccactttgca gtaaaggata aacggattgc cagactccat cccagtcact gagctttcgg
    15121 ttttgtacat tggcatcatc aaaaacacca ttagcagctt tttgttcgac ctctgttaag
    15181 ggtttgccgt gtgagtgatg accatgcgaa aaagcaggag cgctaacaat aaagacacct
    15241 aaagcaacag ccagtttgtg aagacgaatc gccaaaacag tttcctccag agtcattgta
    15301 tatgatatgt aatgttatat tgtaacatat gacaaaatgc aacaactctt aggagacaag
    15361 agaatagttc tctcaggtgt gattatctat cttattgaaa taaaatgaat tatattgata
    15421 atttgaggtt gatgacaaac gaaacataac ctgatgtgat ctgcgccgga tagggcgctc
    15481 gcgccgcatc cggcagggat aacgcgtact ttgtcggcaa tatgaagtta tacagataat
    15541 ttgttgtgaa cctgcttgcg taaccggtta aaaagggaac gcaacttctt ataccgtaag
    15601 gctaaaagca gtacagccag cccagcgtag atgagcggct gcggtgaaat aatcttcact
    15661 gaccacagat aatgtattgg ggccaggatc gcgacaagat agacgaaatt atgcaactgt
    15721 tgccagtgct tgccgagttt tcgctgcatc gcctgggttg aagtaaacgc taaagcaagc
    15781 aaaattaccc agctgataat acctaacgtt aaataaggcc gggtgattaa ctccttacct
    15841 aataacgcca gattattcac gcccagctcc agcaatgcgt aactggttaa atgcagtgtc
    15901 gcccaggcaa agcaccataa gcctaacagg cggcgagtgc gtatcaataa cggctgtttt
    15961 gcgtagcgtg ccagaggtgt gattaacaag gtcgccagca aaaatttcag tgcagtgcga
    16021 ccggtaaaat gctgaatatc tttcaccgga tcggcaccca gtccaccgtg attgatcgcc
    16081 cagaccagcc agagaaacgg caacaatccg gcaagatgca ggcaaacttt cagccatgtc
    16141 acctgttttg ctgtcagacg cattcagaaa ttctcccgca aatccaggcc acgatacagc
    16201 gatgccacct ggtcggcgta accattaaac agtagcgttg gctggcgctg tacatcgagg
    16261 atgccgcctg aaccaataaa tcgttcggta gcctgtgacc agcgcgggtg atcaacatgc
    16321 ggattaacgt tggcgtaaaa accgtattcg tcaggcgctg ccagattcca ggtggttggc
    16381 ggacgctcgc gggtcagctt aatactgact atcgatttaa tccctttaaa gccatatttc
    16441 cacggcacaa tcagtcgtac cggcgcgcca ttttgtggcg gtaacgcctt gccataaaca
    16501 cccacggtca tcagtgtgag cggatgcatt gcttcgtcga gacgcaatcc ttcgacataa
    16561 ggatatttca gcccgccgcc gataaagcgg tcctgctggc caggcatctg ttccggtgca
    16621 taaattgttt cgaaagcgac atacttcgca ttgctggtgg gttcggcaag cgccagcaat
    16681 ttgtgcagcg gaaaaccaat ccacggcacc accatcgacc acgcttccac gcagcgcata
    16741 cgataaatac gctcttccag cgggaagcga cgggttaaat catcgtgatc gagggtcaat
    16801 ggttttgcca cttcgccgct gattttcagt gtccatggat cggttttcag gctaccagca
    16861 ttagcggcgg gatcggcttt atccagcccg aattcataga agttgttata gccggaaact
    16921 ttatctgctg gcgtcagtgg caggttattt tgccaggcgg caggcttgct gaactccagc
    16981 ggttttccgg cgggggcggg tgggcgatcg ttccctttaa accagctaag cagatcggca
    17041 tgcgcagcgt gaggcaaaga aagtgcagct gcgctgatgc ccagtgcttt taacacctgc
    17101 cgacgcttca taaagaatac cgactcggcc gtaacatctg attcttttaa aaattgattc
    17161 tttttcataa gccatcacac ttttttgtcg ttagccagca agagccgttt actgttaatc
    17221 tgtcagggag tgtcctccct aacagtagca gagatctgct tttacatatt tatgctttaa
    17281 ctgccacgat aggttgaata cccatattgg ctaagtaata aaggcacatg ataatgctcg
    17341 ttcactttat taatatgaaa ttcaacgggg atctcaggga agaaactttc aagattttgt
    17401 ttcttgaaat agtccccggt tttaaatacg acacggtaat cgccagtagt cgcagtttgc
    17461 tcgggccaca gtgccttaat tcgtccatcc ttatctgttt tggcggtatt aagttgtaac
    17521 cagccgttat ccgccttctt ttcaagagtg actgtcacgt cagcagcggg ttttccggtt
    17581 tgctggttca aaatgtgcac gctaagaatg ttttgttgtg cggcataaac caaagaaggt
    17641 aatgaaaatg ctgccgttgc taccgagagt actaaataac gctttaacat gtttatatcc
    17701 ttgtcatgtg aatgagtttt gaggatagcg aaaaagcggc cagggtaacc ttacagcagc
    17761 attacaattt tgtaatgaac caggctgttt ctataacata tgatttatgg catattattt
    17821 tcatgaagat tttacttatt gaagataatc aaaggaccca ggagtgggta acgcaggggc
    17881 tttccgaagc gggttatgtc attgatgccg tttctgatgg cagagatggg ctttatcttg
    17941 cgctgaagga tgattatgca ttgatcattc tggatattat gcttccgggt atggatggct
    18001 ggcagatctt acaaacgtta agaacagcaa agcaaacccc tgttatttgc cttactgcaa
    18061 gggattctgt cgatgacaga gtcagagggc tggacagtgg agcaaatgat tatctggtaa
    18121 aacctttttc attttctgag ttgctggcaa gggttcgggc acaattaagg caacatcacg
    18181 ctctgaattc atcattagaa atcagcggct taagaatgga ctctgtcagt caaagtgtga
    18241 gcagggacaa tatcagtatt acactgacgc gcaaggagtt tcagttactt tggctactgg
    18301 cctccagagc tggcgaaatt atacccagaa cggttattgc gagtgaaatt tggggaatca
    18361 actttgatag tgataccaat acggtggatg tcgccattcg caggctccgc gcaaaagttg
    18421 atgatccttt tcctgaaaag ctaatcgcca caatccgggg gatgggctat tcattcgtag
    18481 cggtaaaaaa ataatgaaaa gattgtctat aaccgtccgt ttaaccttgc tttttatatt
    18541 gctgctgtct gttgctggcg ctggaattgt ctggactctc tataatggcc tggcaagtga
    18601 gttgaaatgg cgcgatgata caacactcat taaccggaca gcgcagatca agcagttgtt
    18661 aattgatggg gtaaatccag atacgttacc tgtatacttt aaccggatga tggatgttag
    18721 tcaggatatc ttgatcattc atggtgatgg catcaataaa attgttaacc ggacaaatgt
    18781 cagtgatgac atgttaaata acatacctgc tagtgagaca atcagcgcag ctggcattta
    18841 cagaagcatt attaatgaca ctgagattga tgctttacga attaatattg atgaagtttc
    18901 gccatcatta acggttactg tggctaaatt ggcttcagcc agacataaca tgcttgaaca
    18961 gtataaaatt aatagcatta taatttgcat tgtcgccatt gtactttgct cagtattaag
    19021 tccgctgtta atcagaacgg gattacgaga gatcaaaaag ttgagtggtg taacggaagc
    19081 gctgaattat aacgatagcc gggagcctgt tgaggttagc gcattaccga gagaactaaa
    19141 acctcttggg caggcgttga ataaaatgca tcaagcctta gtcaaagatt ttgaacgcct
    19201 aagtcaattt gctgacgatc tcgctcatga acttagaacg cccattaatg cattactggg
    19261 tcagaatcag gttacgctca gtcaaaccag aagtatcgct gaatatcaaa aaacaattgc
    19321 cggtaacatt gaagagctgg aaaatatttc gcggttaaca gagaacatac tgtttcttgc
    19381 ccgggcagat aaaaacaatg ttttggtgaa actggactcg ctttctctca ataaggaagt
    19441 cgaaaatttg ttggattatc ttgaatacct ttcagacgag aaagagattt gctttaaggt
    19501 cgagtgcaat cagcaaatct ttgcggataa aattttactg caacgaatgt tatcgaatct
    19561 tattgttaat gccattagat attcaccaga aaaatcgcgt attcatataa ccagttttct
    19621 tgataccaac ggctatctta atattgatgt cgccagtcct ggaacgaaaa ttcatgagcc
    19681 tgaaaaactc ttccgtagat tttggcgggg agataattcg cgtcattccg taggtcaggg
    19741 acttggtctt tctttagtca aagcgattgc cgaattacat gggggaagtg ctacgtatca
    19801 ctatctcaat aagcataatg tgttccggat tatgttaccg caaagaaatt aacatagggc
    19861 ttcagtacgc cataaatcaa ccatgaaaaa acgcagccac gtcgtgagta ttaacgtggc
    19921 tgcgattgat tatgcgctta cattcatacg taacagggat taacccgcat aagctgccag
    19981 catttcctgc gccgccagtt tacccaacgc attcgctgcg aaaggactgt cgccggtgag
    20041 aactttacgg tccttatgta ctcgcccggc gatgtcgtca ttaataatgt tcatgcccat
    20101 tttcttcagc tcttcgccga agtaccaggt gagatgaccc ggcatatagc ctatctctgg
    20161 cgtttgtttg tctgcggcgt ctgggaatgc gcaaatggaa taaccattca gtgggttatc
    20221 gccgtggcga agcgccagaa aagccgccgg gccgtggcaa agggagataa caaaacggtc
    20281 atttttgatt gcccactgta aagcagcagc cacgtcctgg ctttctggta gaccaataag
    20341 tgcgccatga ccaccaggaa caaagattgc tgcatattcg ctatcagcgt tgaggctggc
    20401 gacaacatcc gcgagtttct tcggattgcg gaacaacgat ttatgctgct caaagaatgg
    20461 cattactttt tcatctttgt gcggcatagc ccagtattca aacttggtca tcagaccgga
    20521 aatggtcgcc acttcgaatt cgaaacctgc agcatggaga tgatacaacg gcagcaacgt
    20581 ttcaatcgga tggttaccgg tcgagaacag ttttccgtta tcggtcggca aataacgttc
    20641 gtccgcggcg atcaccagaa ttttatgttt accgcgatac ggttttggat agtccacgcc
    20701 atcaagatca gagacgggac tggtatattg gctaagcgaa tattctgaag gaaagaatgc
    20761 attatcttca gcaatatcga cctgcggatt tttacttgtt tgaacagtca tagtgtattc
    20821 cttattgttg cttagttagg gtagtcacta tatttgcgac tgagctggtc gctattttta
    20881 accaggctgg aaaattgagt caatatatcg aaagcggtag tcggcgggtg taagtatgta
    20941 atgatgatgc tgcactggtg ttgaatatta ttcacgccaa attttcctta ttgagcaacg
    21001 tcgtcgcaag tggcctcaat gattttagac cattatcaga ggtacggggt ggcggggaga
    21061 gatttagtgc tgtggaatat tgtggtgctg atgaatattt atatcgcgga ttggtagaga
    21121 aaaagcttat ttcatgcact atttttgcaa atatttctat atgggaagcg tgtttgttac
    21181 ggattaatac ataaagaaat gcatttgggt gttggcttga agattagctg gtagaagata
    21241 ttgtgccagc aggggcaggg taaggggctg tatcgatatg ctttggcaat tgcgtgtgcc
    21301 gcagatggag atgaaatgcc aataaaaaag cctgagttcg atgagggacg aactcaggct
    21361 agtgttaagt tttacagtaa cacgccgcgg cgcgtcggct gattagaact ggtagacgat
    21421 accaactgca aactggtcat cagttgcggc atcggtaatc gctgcatcgt ctttatccag
    21481 caggttaaat ttgtatgctg tgtagacgtt catgttctta ttgaagtagt accaggtacc
    21541 aacttcgata taatttacgc ggtcagcatc aacaccttta tgtccattac gtgcataaag
    21601 atctttgcct ttggaatata cgtagcctaa agacggacgt aaaccgaagt cgaactgata
    21661 ctgtacaaca gcttcaaagt tttgggtttt atttgcaaaa tgatcattag ccaaaacggt
    21721 catgttgcgg gtttcaccgt acatcattgc tgcatagata ttatttgcat catacttggc
    21781 tgctaatgac cacacttcag cgttatcacc gtagccgtca ccagcttgtt cattagtacg
    21841 atcagatttg ctatatgcac ctacaaaacc aaaaccttcg atgttgtagt ctacagacaa
    21901 cgcatagcca tcaccgtttt gtttagtaac gccacgattg ctgttcttac cttgatattg
    21961 cacagcaaaa ttcagaccat caaccagacc aaagaaatca gagttacggt aggttgcaac
    22021 accgttggta cgaccgttca tgaagttgtc agcggaagcc caggaatcac caccccattc
    22081 aactaacata tcggtataag ctgcggcatc ataagcaaca ccgtagttac gaccatagtc
    22141 gaaagatcct aactcgttat atttcaaacc tgcgaaggtt aaacgggttt tttcctgatt
    22201 agagccttcg ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac cagtcagttc
    22261 gctggtgatt tgggtttcac ctttaacacc gaaacgagca taggaggtat cttcgttttc
    22321 gctgttatta tcatcagtat ttgaccagat gcgttcgcca accatcttgc cgtagaaatc
    22381 aactttattg ccatctttat tgtaaatttc agccgcattt gctgcgccag caactaataa
    22441 cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg tgattataat
    22501 cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gcacatttgg atactattct
    22561 atgaag
//
LOCUS       DAZFFI010000074        22345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFFI010000074 DAZFFI010000000
VERSION     DAZFFI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.074"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..538)
                     /locus_tag="ACHJ12_003279"
     CDS             complement(65..538)
                     /locus_tag="ACHJ12_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4991020.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(672..872)
                     /locus_tag="ACHJ12_003280"
     CDS             complement(672..872)
                     /locus_tag="ACHJ12_003280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991021.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(872..1704)
                     /locus_tag="ACHJ12_003281"
                     /pseudo
     CDS             complement(872..1704)
                     /locus_tag="ACHJ12_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(1882..2013)
                     /locus_tag="ACHJ12_003282"
     CDS             complement(1882..2013)
                     /locus_tag="ACHJ12_003282"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4991022.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            complement(2107..2568)
                     /locus_tag="ACHJ12_003283"
     CDS             complement(2107..2568)
                     /locus_tag="ACHJ12_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO4991023.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVCLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            complement(2644..5160)
                     /locus_tag="ACHJ12_003284"
     CDS             complement(2644..5160)
                     /locus_tag="ACHJ12_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991024.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKMMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEALLPELPA"
     gene            complement(5281..8403)
                     /gene="agn43"
                     /locus_tag="ACHJ12_003285"
                     /gene_synonym="flu"
     CDS             complement(5281..8403)
                     /gene="agn43"
                     /locus_tag="ACHJ12_003285"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO4991025.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     IQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGMATTGKGIQVVEAINGATTEEGAFVQGNRL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVHLEGDLMRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASS
                     DNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNTGYVKFGHG
                     SAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPVNWWVQPSVIHTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(8735..9607)
                     /locus_tag="ACHJ12_003286"
     CDS             complement(8735..9607)
                     /locus_tag="ACHJ12_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4991026.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILPRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            10949..12439
                     /locus_tag="ACHJ12_003287"
     CDS             10949..12439
                     /locus_tag="ACHJ12_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4991027.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(12561..13133)
                     /locus_tag="ACHJ12_003288"
     CDS             complement(12561..13133)
                     /locus_tag="ACHJ12_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991028.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(13219..13425)
                     /locus_tag="ACHJ12_003289"
     CDS             complement(13219..13425)
                     /locus_tag="ACHJ12_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4991029.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            14282..14506
                     /gene="hha"
                     /locus_tag="ACHJ12_003290"
     CDS             14282..14506
                     /gene="hha"
                     /locus_tag="ACHJ12_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4991030.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(14838..15035)
                     /locus_tag="ACHJ12_003291"
     CDS             complement(14838..15035)
                     /locus_tag="ACHJ12_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991031.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            15234..15803
                     /locus_tag="ACHJ12_003292"
     CDS             15234..15803
                     /locus_tag="ACHJ12_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO4991032.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            16064..16447
                     /locus_tag="ACHJ12_003293"
     CDS             16064..16447
                     /locus_tag="ACHJ12_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4991033.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTTPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            16444..16869
                     /locus_tag="ACHJ12_003294"
     CDS             16444..16869
                     /locus_tag="ACHJ12_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4991034.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AHGAWFEHDLHALSEQQKVDCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYSRATCTKSVYSYQPDLYQDCGGAST"
     gene            17118..17315
                     /locus_tag="ACHJ12_003295"
     CDS             17118..17315
                     /locus_tag="ACHJ12_003295"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4991035.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            17343..17909
                     /locus_tag="ACHJ12_003296"
     CDS             17343..17909
                     /locus_tag="ACHJ12_003296"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4991036.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            17956..18229
                     /locus_tag="ACHJ12_003297"
                     /pseudo
     CDS             17956..18229
                     /locus_tag="ACHJ12_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18295..18492
                     /locus_tag="ACHJ12_003298"
     CDS             18295..18492
                     /locus_tag="ACHJ12_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4991037.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            18502..18696
                     /locus_tag="ACHJ12_003299"
     CDS             18502..18696
                     /locus_tag="ACHJ12_003299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991038.1"
                     /translation="MDVATNRGILGGQQHETCCNAGTLAMCKMPALVLWNPALPLVLA
                     IFPFIFLLIPSVQQRVVIPS"
     gene            19223..20347
                     /locus_tag="ACHJ12_003300"
     CDS             19223..20347
                     /locus_tag="ACHJ12_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO4991039.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKLL"
     gene            complement(20489..21763)
                     /locus_tag="ACHJ12_003301"
     CDS             complement(20489..21763)
                     /locus_tag="ACHJ12_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO4991040.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT         5434 a         5441 c         5676 g         5794 t
ORIGIN      
        1 ctgcgctcgc cgggcgtcat cgcgccgggc agaaaggaaa gttgttgcat tgtacttctc
       61 ctgctcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc
      121 gcagccggta ataatggacc gtcatggcat aacactccgt acgacaggcg tgatgactgt
      181 acgtcatcag acaggcggca atgccggcgg cttccgggct catttcagcg cggttaccgt
      241 tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg ggtgccataa
      301 atgccccgcc gttgttcagg gtatacagat tccagatacc accacagtag tcttcgcaaa
      361 gacggtccat ccagccgaaa acacggggct ccagggtcac ccactgtgga atgaggccaa
      421 aatgctgcgg ccagaagctg atgcgctgtt catcagggac tatggtggca accagttgtt
      481 gcaggccagt ctccggtgca caggctgaag aggtggtgtt ctgagacaat gttttcatgt
      541 gtgaaatcct tgtaatgtga ataaatgaga ggaaataaag gccgggggct gacaacatca
      601 cgttcatcac tgaccgtgag acccggcaat atcagggatg aatgatattc ggctgaaatt
      661 ccggacagcc gttaaccgtt ccggtcacag acggatggcc agatgccatt gcctgtactc
      721 agtctgcaca gactgagttc tccgggacgg taaagcagac gggtacacca ggcgcacagg
      781 tcatcatcag aaatggaacg acatcccggc agcgcctccg gggcgtcggg tgatacgggt
      841 gcctgaatca cgccttcagg gatatgtttc atcagcgcat gctctccagc agcgtttctg
      901 ccatcaccca caatgcgcgg ttgagcttaa tatcggttta atatcggtgt cgatgctgtg
      961 aatggcacgg gtgtggatac gttttccttt tgcactgcga ccggaaatcc cgcctttcag
     1021 catattctcc tggatggtct gatatgcgct ccacaggtct ttaccgtaat cctcccgacg
     1081 tcgtggcgtc agaatgtcgg cggtggtgac gggctgatgt tcgtcaccat aacggtaagt
     1141 cagtgccgcc tgtgccagcg cctggcgtgc cggtggcggc aggaccagcg actgcatggc
     1201 atcacgtttc tcttcaatcc ggtcaaaaac gcccaccacc tcgtaagccc cttcaatgac
     1261 tttctgcacc acatttcccc ggtgtggaac acgcacttcc cccagagact gaccacagac
     1321 gcacccgttc tggcagacga accggaagta acccggcagc atctggtagc tggaggtacc
     1381 gtcatgggag ttgagcagaa taatttcagg gacatgttct ccgtttatct ctccggcccg
     1441 ccgcagacgc agcatgtgtt ttgtgtatcc ccggcggccc gggtcgcgca cacgggtctg
     1501 gcaggcgaaa aagggctgga agccttcccg ctgcagactt tccagtacgg tgatggtggg
     1561 gatgtacgta tagttctggc ttcgggatgt gtgtttctct tccccgaaaa tccccggcac
     1621 atggtgcatc agctcttcat gtgtcagcgg acggtcacgg cgtatctggt ttgcagcata
     1681 accaaaacga ctgactaatc gcatactttg ctccttatcg gtggttaaga tttactggtg
     1741 caataaatga aaaagccgcg tctcccggag aagacgcggc ctgatggacg aaatcaatga
     1801 cgtttattgt ctgagaagcc ctccactggc gagccacgca ttaagctgtt ttccggcatc
     1861 agcttcagag ttccggcagc gtcagcgtcg ctgaccttcc gcgttgcgga taacccgacg
     1921 gaaatgcatt cccgggtcat cctggataaa cacattgcgg taagcggtta tgacagcaac
     1981 cgcctgcccg tatgagaaag atccttcagc caggacatac tctgtgtgta acccggcata
     2041 tctggtttct cctgataaat agcctctgcc atacgttgtg gcagaggctg aagcatgaaa
     2101 ctgacttcag ggatcagtta acatttttcc ggaaacggta atcagcagtg gatggtagtc
     2161 ctggggatcg aaaaccgata acggcagaca gacacgatgg ccgttacttt cttcagttgc
     2221 tttaatgatt tcggttgtgg cgacattttc cacgcactcc gtttccagaa atgcgtctgt
     2281 ggttcgcgtg gcattactgt caccaaaggc ttccgtttcc atttttctgg tcaccagcgt
     2341 ctgaccatat ttgtctttga gttgcagagt gatggtgagg gggccaaatc cttcatcgtt
     2401 tccgccatta tccagccgga actggtaagc acaaatattt cccgggagcc atatcgtatc
     2461 tgtattgcgt atactgatgt aacgttgatc ctgtgcccgg agtggggcag accacgttaa
     2521 ccccagaatg aaggcggtaa tcatgcaggt tttgaacagg tgaatcatgg tatttacctc
     2581 tctgagtcat gacgatgaca ctgacaaatc aggtgataaa acgtaaaagg cgcagaatag
     2641 ccgttatgcc ggtaactccg ggagtaatgc ttcttccagt cggttaacca tcttgccgag
     2701 atgggatgca tcatattcca tgacggggcg ttgcctgatg atactgacca ccagtggttt
     2761 gattaacata ttggtcgcgg cccgttgttg tataccggcg gcgaaaatga tctccagagc
     2821 attctgatcg ataacacggg ccagattcgc gttcagattt tcaatctcat gtccggtcag
     2881 caggaaactg ccggcaagcc atgtcacctg ctcctgaatc attgccagaa ttttgacgcg
     2941 atcttcttca accagcgctc cggcaatttt tcctgataca gcggaggcaa tcattccccc
     3001 cagaacaccg ccagccacac gccctgtcag tgcaccaaat ggtcccagtg gactgaacag
     3061 tatcccgccc gccactgacc cgacagcacc acctgccaca ccggaagatg ccacggcaag
     3121 attcctgatg aactgcgcac cggagatccg tccccgcaac attttgatca tgtcagggcc
     3181 ggttgtgaca gcgaccagta ccagagatgt caccagcgta cccttcatca ttttgttcag
     3241 ggcactgata tttcctgcac cggtcccctt ctgaagcgcc tgccggacag tcgggctggc
     3301 cgtcgaaaaa tcaatatgct tcagcatacc ctgaacaaca ctgagccggt ggagttgctg
     3361 ggttgtgacg tagacagcca gtgtgcgggt gaaggttttt cctgcctgga cagcagctgt
     3421 ctgcagggca gcatcgcgat cgccggtgct gagccagaag accgatgccg tcagggcaaa
     3481 actgatccct ccggccgcca gactgacaac cgaaccctcg gcaatatcat aagtgaccga
     3541 ttcgatggtc ccgaaccggg tgatattacg ggcctgagta taagtcagat gtccccgacg
     3601 aatcagccgg gacgcttctg cgggatcggt tacaccgggt actttacctt cgcggatctt
     3661 attcctcatg gtttcaacag cgccggcata ctgatcacgg gggacttcca gttgcatggg
     3721 gccattattt cccatgtaac gatactgtcc gttttgtccg tcgaatgccg caccgacgct
     3781 acgggctgca gttgaacaat atttcgtctg gatttctgtt ccgctgacca gtctgtctgc
     3841 tccatttctg gcattattat caccgaggat ccgtgcgttt ttcagacgga ctctgtcgac
     3901 aatatggttt ccccgttcag cggcaaaccc atgtccctgc cctccggaag caaacatttt
     3961 ctcatttgta ctccaggttt ccggcagttt catgagaatc agaccattcg ccgttgcatc
     4021 ggaaataaag ctgttgtaag ggcgttcata aatttcctga cacagtgttt ctgtttcgcg
     4081 acaaagagag gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac
     4141 ttctgtcagc ccgcaggagg cacagatacg aagctcatcc acgtgatgga atgatacaaa
     4201 acggcgacct tctgaaaagg gataccagtc accgccgtcc aggcaacgat cgagaaaatc
     4261 gtcattcgct tccggtgata aattcaggcg gttccggaag agcgttttca gttcatcagg
     4321 tgtgaatgac aggttctctt tcaactgttc gttaaaccct gccatgtttc tttcatacca
     4381 ggcattgttc agtaaccagt ttccgtctgc cagttcaaat tccctcagct ctccccggga
     4441 aatccgttga gaaatgagcg tcagccagat gcgctccgtc agtgagtcag gaaggctgta
     4501 atcgttttgc aaactggtca gccacgattt actgttgagc accggttgtt cttccagtgt
     4561 ctggctgatg gcacaggtca gttttttcag cgcctggatc tggctggccg cccatagcag
     4621 cgtcactaat gcagccagcc agtacaacaa cccgccccac cacatcagtg acggtgaaat
     4681 atcgtactgt aacgcgatca atggtgcgca tgcagaacac gccataagga aaaaggcagt
     4741 ataaaaacag cagtaatacc cgttaatttc cttgctgata tcttctggtg tgatcccgtt
     4801 ctgctgaatg tttttgatct gcaggggaac agccagcaga ccaccggcga gagcggtggt
     4861 cagaataaat ttcacagagt caaaggtgtg agcctccagc gcgatatgtt taccatcaag
     4921 tccccacatg aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg
     4981 aatatggaac aaccgggtta tgcgttcggt gatatagcca gccagtaagg ccgggccgaa
     5041 cagacacagc aatataagcc ctgccagcgc agaagccgtg ctgattaatg ctctgaacaa
     5101 aaggcgaaga atggcaaatc ctgctatcag caggatcagc gcgccgacta tctgtaacat
     5161 agtcactccg tgatgaatat catgttattg ttaatgccag cgaatgtggc gttgaatatc
     5221 cgttgcgccg gtccggatcc cgatcatgat gaccgggacc acagagaggc gatggtgctg
     5281 tcagaaggtc acattcagcg tggcctgacc gttatagcct tcggcgctgc tgccgctgac
     5341 actgtgggca taaccggcct gaacgcccag ggtgatattt tcccggacac gggcttccag
     5401 tccggcctgc aggtccagtg acgtgccatt ccgggacggt gagaacgtca tgttactgcc
     5461 ggctgcggct gtacccatgc tcatgtctcc ccgggagctg aaggtgtgga taacagaagg
     5521 ctgtacccac cagttcaccg gcaattcacg cacactgtgt ttggcactgt cgcgcagggt
     5581 gtcacgggat gaggtgcctt taccaaagtt catatcgttg tggctgccca gacggaaacc
     5641 ggcacgcaca tgttgtgcac tgccatgccc gaacttcaca taaccggtgt tatcctggcc
     5701 gtcatccagg gagagtccct gccaggtgta ctgcagttgt ggttccagca tcagattgtc
     5761 agtgatactg aagggcagac cggtttccag tgagcccagc cagccccagc cccgggcgcg
     5821 gaagtcgtta ttgtccgatg atgctttcat gctgtggcgg gttccctgtg ccacaatgtc
     5881 agcccacagg ccggaggatg tgtgtaccag attcaggtat ccgcccagac tgccggcatc
     5941 atcccgaacc gtgccggcgc gggagccgtc atcatcctta acatcaacgg aagaatggcc
     6001 tgcagcacca tatatccctg cggtcacaga cataccggca acctctgttc tcatcaggtc
     6061 accctccagg tggacgaagc catagctgcc gctgctttcc ggcgtggccc cacgggcaat
     6121 accgccattg ttatcgtgac cgagatgacc gccctgaatg ctgagacgga cgctgttatt
     6181 ttcaccgctt acaccggtct gatggctgcg ggagcctgcc agaatccggt catagtccat
     6241 tgcctgtgtc agcatggagg catacagggg gacttctgca cgataagcat tttcactgcg
     6301 cagataccag ctctcatcac tgtcccggtt gagggagtag ttaaaggcac cggcctgcag
     6361 cctgttcccc tggacaaagg ccccttcctc cgtggtggca ccgttaatgg cttccactac
     6421 ctgaataccc ttaccggtgg tcgccatccc tgacgcactg ttgccggcgt tcaccaggtt
     6481 caggatggtt tttccggttg ccctgccgcc gtcaatgacc agtctgtcag cattgttctg
     6541 tgccatatcc gggcgtacac gcaggctgat ggtgccattc tgtccgttca ggtttttcac
     6601 tttcagggtt gccggtacga acttccctgt gcgggtggag gtgaaatgaa tctgtccggc
     6661 atggctgagg tcatccacca ccgactgcac cgtggcgtta tcggggatat tccaggtggc
     6721 accggaggcg agagtgacat tcgtggggtc aatggcaccg ttcagcactg tgctgccggt
     6781 cagcttcagg gccgtgccgc gctgagcgat gacatccgtg gtgacggtac tgtcgttcag
     6841 cgtcagcgtg ccttcattca ggttgacggt tttctggatg agtgtggtgt tgctgacagt
     6901 gagtgtgcca cttcctgatt tttccaccct gccgttaccg gtaagagcgc ctccctgcag
     6961 gagtgcatca ccttcatgga tggtcagggt atcgttattc accgttttcc cggaaagggt
     7021 aagagtggca ccgttattca gcgtggtggt gcccgccagc gagccccctc tggcggtgaa
     7081 cagtccgcca tttaccgtgg tatccgtggc cgtatcaccg gcgttcagtg tgaaggaact
     7141 gcctttttcc agcatcaggg catccgcctg accgcctccg atactgaatg cctttccgtc
     7201 gctccgggta ccactgacag cggccccgga atcggccagc agtacaccgc cattccccat
     7261 ggatacggtg gtggcggcac caccgttgcg gacatccagc gttccgccat catccacacg
     7321 ggtattagtg gctgtgtgtc cgctcagcac atccagacgt ccgccatttt ccagtacgac
     7381 attatcagcc ttacccgcca caacggagaa tgctcccagg cggtttgtac cggtgacggt
     7441 tgcagctgta ctggtaacca gtgcaccgcc cgtgttctgg gtgacatcag acgctttacc
     7501 gccggcattc acctgcagct ttcctttctg gttgatggtg gtatgcgcgg cagttcctcc
     7561 ttccttaatc acctgccagc catcacggtt tatcagcgtc tctgttgccg tgccaccgtt
     7621 gtgtacatac tggttacccc cctccagccg ggtgttatgt gcctctccgt gtaccgtctg
     7681 gtcaccaccg gcgtaaatga gggtgtcccg tgccatcccc gaagaccaga taacctgccg
     7741 gccatttttg ttgatggtgg tggattcagc cgtccctccg accatctgac cgctggacac
     7801 attttcagac tccggaccac cttctgcacc ggtgttgacg atggttccgg ttgccagtcc
     7861 gccatgtttt atggtctggt aaccatcctg gttaataatt gtaccggcag ctttccctcc
     7921 cccgtgtacc cactgctcac ctctgttatc cagcgtggtg ttcaccgcca gtccgttaag
     7981 gctctgccct ccgccatcac gaataaccgt atcactggca gttcctcctg cctgcacaat
     8041 ctgacgacca tttgcggtga cagtggtgtt tctgcctgtg ccacccgcgt ttatccattg
     8101 cccgccggtg ttttcgtcac tgtccggccc cagctcaagc ccggtactga cagtcacgcc
     8161 atcagctgtt ccggatacaa actggttgtc atggtttacc agtgttccgc cattcactgt
     8221 ttcacccggg tgcacaacga tgtcagcagc cagcaccggg agtgacgtga ccgcggcaag
     8281 agacagtgca accgccacac cgccacgttt accccgtgcg cgggccagtt cggaggcaac
     8341 cacgaaagcg cccgtaatgt gattccatac cagcctgtag caggtattca gatgtcgttt
     8401 catcagcatt tccttaatca gagtgagggt gaataaaaaa gccggtatcc acagatgtgg
     8461 ataccggcag gtaaagggtt acagtgccgg aggtccggct gtgggagttt ctgacgtgtg
     8521 gttctgttgt tccctgaatg tctttctgta cgtatctgtg catgttactg acaaaggtta
     8581 ttattagcct atcgatcgat ataaacgatc gataaaatag atatcgatcg ttttattcta
     8641 ttattgagct attgcacgga caagagagac agtaacgaaa acagtgcttt tgtgagcaga
     8701 ctcagagtca gggagggcat cagtcacgac aggttcagaa gaaaatccag ttccataccg
     8761 cacgggcaac agagaccacc gtgtcacgaa cagcgcgcag ggccgtacgg acaacagagg
     8821 caacacagac gctctcagct gtgtcaaata tccggtccac cgcaccggta aactgttcac
     8881 gggcctgaga gcggacagat tccgtgcgca gctcgtcctg cagtcgggtc atcaggggac
     8941 tggcggcatg gtcgggaagc gctgtcatga gcgcactgac cagcgtatcc agttcccagc
     9001 cggtgcgggc cgatacggcc acaaccggat gtacgggccg gaacagacgg aataccgcct
     9061 ccgttttttc gcgaatattc tgtgcctgtg caggagaagg ctgaataccg gccatatccc
     9121 attcatggca gggctccgtt ttatcggcct gcgtcaccac aaacagtacc cgctgatgtc
     9181 cccggggcag tatgtgtcgc cagaaatact catccacaga cagggcacgg tcatcggctt
     9241 taatcagcca cagtaccagg tccagttctg gcagaatgtc acggtacagg gcttcatact
     9301 ctgcatccct gtcccggctc tcgcccaccc cgggcaggtc agtgataacc atgctgtgac
     9361 cgtggccact cagacggaag cgccgcactt cccgggtgcc ggcgtgaaca tcactgaccg
     9421 gggtgacctc cccctgaaac agtgcattac agagtgaaga tttaccggcc ccgctttttc
     9481 ccataatgcc aatcacgggt tcgtggctgg tgagtttgcg cagatgttcc aggatgtgac
     9541 gggaaagtga gtaaggcagg gaggagagcg gtttttcaat tgcctcaatg gcatcagacg
     9601 gattcataca tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga aaggtggcgg
     9661 gggatccacg gagataatat ctgtctgaaa ttgttttgaa tcgatgggat tgttgcgtat
     9721 taacatgaag gagatcagga cttatccgtt actgggtgat ctccttccat gaaaatacgc
     9781 ccttattgtt aaacagcagg ctgatttctc cgtaaagggg aaaatattta tcggctgcat
     9841 gattatcagg tgtgtctgtg tttactgctg tgagtatttt tcagtgtatt gtgggggaga
     9901 tgtcagtggg agatattaaa tttacgttta taattatctt acttcttctg tgtgtttttt
     9961 ataagagtgc acacgtaatg atatctgttg ataccatgtt gttaagtgtt tcccttccgg
    10021 gtcatgcgta cttgcaaaat ggcatctgaa tcgatcacgg tttggccaaa ccgtgatcga
    10081 atagagagga atacgcacgt aatgattaaa tgcttttgtt acaccattct tccggcatga
    10141 atatttatcc agttcccttt tttatttgtg tagtgatact gtcggaatag cgttcagtaa
    10201 tgtgaagagt cctgttaaaa atctttaata aatgtgtcac cctggtcatg aaatatctgt
    10261 tgagatatta ttagttggtg gtgtttttac tgagtaatag ctggctttta attcctgaac
    10321 tgaaatgctg tcgctcgtca gggtttaatt aaaactggat gatgatgtga aaatgaaggc
    10381 tggtttttgt gtttgtcgag gaagtgttgt ttttatccat tctcatcatt ggctggctgc
    10441 gattatacag aagtatttga agaaaaaatg gtgcatttta tctgtgccgg cagggagggg
    10501 gattttcctg atatgtcagt attaaatggt ttttgttatt tataaaaatg tcatcctgtc
    10561 catagaatat tgtactgagt actcagtatt cagcgcagga actgtcatct tctttatttt
    10621 attcttcttc aggagggcag ttttctgaaa gcgttactcc ggggaaactg tttatgttta
    10681 ttcccggcag gaaacccgta cctgcaatac cctggatgat gctttttgct ggtacgttgt
    10741 tgtgtgaaaa ttaatcaaca tgtgtgctcc ggagagcgcc cgtagacgca cagattcatt
    10801 ctgacattct ttagcgacaa aataccgggt ggttattatt taacctgaaa atcgacagaa
    10861 atttttatcc tgtattgatt ctgctgctat ttttgctatt agtagcaata gttgcggtac
    10921 tttatgttct gtgtgttgtg aggtcattat gccatacttt cagtatcctg atgagtttcc
    10981 cctgagctcg ctgcctccac tcattcgtga tgccgttatt gaagctcagc aaatcacgca
    11041 ggctcccctg gggcttgtgg cagcatctgc cctgggcgct gtttcacttg tctgccagaa
    11101 tctgattgat gtctgcagac tgaatacact gcgggggccg gtttctcttt tcttactgac
    11161 gcttgctgag tccggggaac gaaaaaccgc agtggacaaa ttattaatgg agcccctgta
    11221 ccagcaggag atgctgttat attccagaca caaaaatgaa ctgacgacat ggaaaaataa
    11281 ggaagagctt ttaaaggcgc agaaaaaagc attgttgtca aaactgaata aagaactccg
    11341 caaaggcgcg gatgagtcag aaacgctcag acaactggaa gcgcttcaga aaaacagagg
    11401 ggaaaaacct gtaagatata aattcatttt taatgatgcc acgacagcag caattaaaga
    11461 tcagctttgt ggtcagtggc gttcagtggg tatcatgtct gatgaagccg gtataatatt
    11521 tgatgggtat accctgtctg agctgccttt tattaacaaa atgtgggatg gctccgtatt
    11581 gtcggtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa tgacattatc
    11641 cctcatggta caacccggtc tttttgaccg atatatggag cgaaaaggtt ctgttgccag
    11701 ggacagtggt tttcttgccc gttgtctaat cagtaaacct gccaccacgc aggggaaacg
    11761 ctttattaac ggagctgtga ccccgggagg atcattaacg gcatttcatg aacgattaat
    11821 ggaactggcc agaggcagta ttgaaaaatc cagtaaggat gaacgttatt gtctgcattt
    11881 ttctcctgaa gcgcaaaaaa tatttattga tcactataat gttctggagc aggatttgtc
    11941 tccttccgga cccttaagcc agtttcgggg gcatgtttca aaaaaaacag aaaacattgc
    12001 gagaattgct gcgttattgc agtatttcag tcagggtgaa gggaaaatct ctgccgatat
    12061 catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa aattatttgc
    12121 gttacctgat gaatctgaac tgcagctgca ggacgcccgg gagctgctcg actggctcat
    12181 tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc tccagtgtgg
    12241 cccgggccgg ttcaggaaca ggaaaaagct gaacgtcctg ctgaacattc ttgcatctca
    12301 actcaggctc tctgtcgtgc aggaaggaaa aaccacctat gtcctgcttt ccgaaataaa
    12361 caatatcacg ctggataaac tgaatgcgtg ctacagtcag aaactgataa gatggcatga
    12421 gcaatccttt ggcttataat gttccctgtt gctgagacgg attattcagt ggtggtatcg
    12481 cagtatgccc gtcacccccc gcttctgcat gagggtgacg gaggaaaagt aagtgtgcgg
    12541 aggcccggga gcgttatgaa tcaacaggcg tataacaggt actcgtcaat catcagttcc
    12601 agctcgcgct tttcccgttt gcgcgccaca gcccagtttt caagctctgc atgtgattta
    12661 tgctttaact cttcagtcca gggctgcatg gagtgggcac acatcagatc ataccagcac
    12721 agccatatca gcgtgtggta gagatcacga tcgtcgaaac gccggagcag gctgttcagc
    12781 ctgtttgcca gccggttttc acccagacga tgtagctcac tgagtaaaac gccgcccaga
    12841 ttaaaatgtg taagcagcat gatttcatgg gtctgaatgc tggtcattaa cccttcctga
    12901 atggcaggaa tgaacggtgg tgttcccggg ttgacgacct gatagagtcc ctgtggtgcc
    12961 actgagatga tagtgccttt ttccggcaga aaaaagtggg ccagggtcag gcgatgataa
    13021 ccatgaagat gcggagaccc cattccatga gcgacgataa gcctcgtcac gcttgtcatc
    13081 catttctgat gctggatgag cagcagctca caccggtttt caagtttttg cataatattc
    13141 tctcctttag cgcgcagcaa aatgcctccg ggggccggat catgcctttg ctgcggttga
    13201 acggataacg gtcaggaatt acggacggga ctgtgcaata cgcgcctgca gccaggtttc
    13261 cacttcactt ttcagccatc gggagctacg gcccagtttg atgggggcag gaaaggcacc
    13321 atccttgatg agcttgtaaa accatttatc ggtcaggccg gtcagttgag tgataaatgc
    13381 catgtcgacc atctggtcat ccatcagtga aactggggta ttcatgaaaa tgttcctccg
    13441 gatgttgtta atctcaaccg gcgttggtga tgaaaacaac gcaccacctt caccggcata
    13501 acaaccgggg agaaacgtaa aaaataatcg ggaatgtgac cgtcaggaga taaacggggg
    13561 gattgcggtg gctgagccgt accggtatgt cgggtgattg ttgcgaaacg atgcttccgc
    13621 acgcgcacgg gaggactttc gtgagacaag gggaaaactg gcgttctgat aattgcagag
    13681 ttgtttttga aggcggttgt ggtgcatcag cctgtgacct gtgggcaggc gacaggtatc
    13741 actggtggga taagactgtc tgattggtat tataaatata taagagagct caaacacatg
    13801 agttcatata tgtgaaataa atctgtgagt gtgatcagca gaccagtcta cccagtagca
    13861 acagattgcg ttgtactcac agggttatcc ctgataactg gcgtataacc tgcgtacata
    13921 aaacgtacct gcaatgcacc tgaacagagt gcattaagca tccgcctgag cggtaaacgt
    13981 atccgatctg gtgagtgaca gtctgagagc attctcagta atcgccccct tgtatcctgg
    14041 aaattggtta agagcgatta gatttggcaa ggtgtatgca tccagcagct taacacctca
    14101 gctaactggc tggttacctt caggttgtcg caatggcatc ggacatccgg tgtcggataa
    14161 ctaaacgtag cgttaaggcg atattgctga gagaccggtt aagtggtggc acgggagtaa
    14221 tgagtttggg gagggtacca gaatgccagt caggtgcggt atgctggcag tatcacgttg
    14281 attgcagcat aaagtaacga gagacgaatg gaagttaaaa ccagagcagt tccgtcgttg
    14341 ttcatcccgg gagacactgg aaaaagtgat tacccgcaca cgttataaac ttacccctgc
    14401 ggagctggaa gcctttaact ctgcggtcga tcaccggctg gcagaactga caatgaacaa
    14461 actttacgat cgcgtgccgg cttccgtctg gaaatatgtc acctgaatct tgaatccgcg
    14521 ctacgatgac tctggtgtta tctgtgcacc ggaagatcgg gaacattaag cagtataacg
    14581 gcaacaaaga attcgaaatc aaaagggaat ggtgtacaga ggggcaggcg ttcaggcatt
    14641 accggcctgt atcttctgaa ggggactcgt atttgtcggg gctgagtatt ccgctcgatt
    14701 cgatcacgga ttggcagaaa cctgactgtg attgattcag gtaccatttt gcagggatcc
    14761 ggtgcgttac tgtctggcac aacaaacgca gcgggtcgct ctgccccgcc gattcctgat
    14821 aattcctccc ctgtaaccta ctgcaacagt acaaatacac tgacgcctga aacggtaacc
    14881 agaatcagca ccagcagtaa cgtcagtaaa cagagcagac gatcggtctt ccggtaataa
    14941 ccgaaggggt cgacagcgcc acaatacggg caacacactg tattatgagt aattgcgttt
    15001 ccacagtccc tgcacttctt tttgcgtatc acgatcgctc cctctccacg ccttatccgt
    15061 aacctgtttt ttacattaaa aaaacaacgc cgggaattca atcgtcagtc cggagacggc
    15121 cgttcgggtt atcacagagt gcctgagaca gtgtcctgcc ggaggtcacc tcaggggctc
    15181 tgcgcatttt ttacgacgtg gctattccgt atgaaccata cggagattta accatgacct
    15241 acaaatacaa ccccttctgg cagcaacgta ttcgtgagac ggtgcggcac gcactggatg
    15301 ttcatccccg cctgacggca cttcgggttg atctgcgtct cccggatgtg ccggcagcaa
    15361 cggacgcggc tgtgatatcc cgcttcatca atgccctgaa agcccgaatc gacgcttatc
    15421 agaaacgtaa gcatcgggaa ggtaaacgcg tgcattcctc gaccctgcat tacgcctggg
    15481 cccgggagtt cggggagctt aaaggcaaaa aacactatca cctgctgctg ctggtcaacc
    15541 gggatacctg gtgtcgtgcc ggcgattacc gcgctccggg gtcactggcc gggatgatta
    15601 aacaggcctg gtgcagtgcc ctgggagtgg atgccggacg ttatgacacg ctggcacatt
    15661 ttcctgacaa gccagcggtg tggctggaac gcgatgatga aaccggcttt cagcaggtgc
    15721 tggaacgttc tggctatctg gcgaaggagc ataccaaagc tcgcggcacc ggtgagcgca
    15781 attttggctg tagccggagt tgaacacgac aggcgcacca atacccggca aaccaaaagt
    15841 atgggttgtt gtctggcggc aacgatgtat ccactcgccc actgactgtt ttttacgtca
    15901 gtgggccatt gtgtgacaac cacagtgctg taacagcaac cagcctgctt tacccaaact
    15961 ccagtccaga ttttaactga accgccattc acaccctgac cacgctgccc gtaccgggac
    16021 ggctgttgcc tgcgtgtcgt ctggcggctg aggaacatat actatgtacg caaaatcctt
    16081 tctcgctctt gatggcaacg gacgtctgac gggggcccgt accgcacaga ctacgcctta
    16141 cgaccgctac acctgccacc tttgcggcag tgcactcaga taccacccgc aatacgacac
    16201 tgaacgtccc tggtttgaac acactgacga cgggctgacg gagcacggtc atgagtgccc
    16261 ttatgtcaga ccggagcgca gggagataca actgattaaa cgtctgcagc aattcgtacc
    16321 ggatgcctta cccgtagtac gtaaagccag ctggtactgc agacaatgtc accacgatta
    16381 ttatggggag cggtactgca cacactgcca gaccggacgc ttcagtgagg agggggtagc
    16441 agaatgaacg gtataaaaac ggggctgggg atcacgccgg gtgaacacat tatcagtgcc
    16501 gactctgcgc tcagcagaaa tatccggcat tgtttctgcc tttcctgcca tggccggttg
    16561 attctgcaga cagatgctca tggggcatgg tttgagcatg atttacatgc cctgagtgag
    16621 caacagaagg tggactgtgt ggtgctgaat ccggaaaagt cccaccctta cattgaggat
    16681 atggcgatgt ttttatcgcc attaccggtc gtgctggagt ggcattgtgt gatgtgtgag
    16741 cagttttttc atgggaagaa gttctgtgag gcctgtggca cggggatcta ttccagagca
    16801 acatgtacga agtctgttta cagctaccag ccagatttgt accaagactg cggaggtgcg
    16861 agtacttaat ccaaaatatc cgctctgcgc tgggcgttca gaggttttgc cagcctgcat
    16921 tttatccggt gtttcgcacc taccccgttc gtgacacaaa ggcgcgacac cggatatccg
    16981 cagtcctgct tacctgaata aaacccgggg tcatgattgt gtgctcgtgg aacgaaatcg
    17041 caccgtgatc gcggattcgc cgaatctgca ctgtggtgtc aataaaccac acgcgagagt
    17101 cctctccgta ttgatcagtg catcaggacg atttctgcat tgttaatcca gggggctgca
    17161 ttattcattt ccctgctaat ggacgttaca tccaggatat taatgctcct gatttccctg
    17221 agcagtatca gcgcttattg gcacgtctgg attatctgac gaagcccgat gctaaagcat
    17281 caggccagcg taactctggc tacagcggtt tttgacctgt tgtaactgta aggtgattta
    17341 ttatgtatac agaatcaaat gttaagcttt cctgtaacgc cctctggcaa aagtgtgtcc
    17401 ttgcaactct gtatcgggct gcagccacac atcctgaaat taaattatta caatttaaat
    17461 taataccagg gcagggtatt aataaggcca ttccctctct gatggctgat tttactggtg
    17521 aactgcatag actcatttct gaatatttgc agtgtaagcg acgtaaagat caatctgttg
    17581 agcccacccc tttatattat ctctggagta gggaagctgt ggctaacgat accactcctt
    17641 atcttatggt gctgctactt aataacgatg tctggtgtaa gagtcgtgag gtggtggttc
    17701 taagaaatct gattgagcag gcgtggggaa aagtattgag tgaagataac tccattacac
    17761 atctggttgt gtatcctgaa gtgttgtcgt tggtatttga tgcagctaac acatacaacg
    17821 ctgagcctgg tgtactggaa cagacagccc gggcttacat tgaatcgcat ggtgctgttg
    17881 aacgtcatct tgggtgtagt gacttttaac gtcaggtgac gcattgtcac ttcagacctg
    17941 ctacctatac gccggaatat aatggtcaga gaggcatatt tcgacgcccc taatctgggg
    18001 cgtttgattc aggatgtatg gaacaatccg ggctttgtgt caccgggcgc atcaaagctg
    18061 taccacccgg gatgtggcgc tggatcgtca tttaccggac tgcgaagaca tacttaaaga
    18121 ggtgatatgg gcgttcagtg attttgtccg cgataatcga ggtgtgtatg atccggaagc
    18181 ccgttacccc gccggtaatc cctggtatcc cgtaacaggc cagttctgaa agcacagctc
    18241 agcagattcc ccgcattcgc acaatcttca taaatctcac aggacattct gaccatgttg
    18301 actacaacaa gccacgacag cgtatttctg cgtgccgata attccctgat cgacatgaac
    18361 tatatcacca gtttcaccgg tatgaccgac aaatggtttt acaagttgat cagtgaaggc
    18421 catttcccta aacccatcaa actggggcgc agcagccgct ggtacaaaag tgaagtggag
    18481 cagtggaagt gaagtagagc agtggatgta gcaacgaatc gaggaattct gggggggcag
    18541 cagcatgaaa cgtgttgtaa tgccggtacg ctggcaatgt gcaaaatgcc agcgctggta
    18601 ttgtggaacc cggccctgcc cttggtgctg gcgatattcc cgttcatctt cctgctgata
    18661 ccttccgttc agcagcgggt tgttatccct tcctgactga ttcgtcattc cattcttatt
    18721 gattagaact ggcattacac cggtgccggc gtgctttcct gcgtgtctgc gccggttcag
    18781 taaattagaa aagaaacatt tgtcagcgcc agtgatataa ggcgtaacgg ctaacaatct
    18841 tggcgttaat acattccgag caaataaccg aagcgttact tttagtgacg gggttatcca
    18901 atacttttct agtcagtgtt tagtatttat gcaagcgata taagtagtca ccgcttagac
    18961 tattgacatt catgatgcct gttaattcaa atttcaggta tgatcagtca tttattgttg
    19021 tgatactgat tccgctagtt tagcataact gtcctgcaaa tcattgttca aaaaagaaag
    19081 ctcctacaaa ctgtatcctg tatctctacc aggagataat atacttggtt atttttgtgc
    19141 ataagctcac ataaaaataa tattaattat gttttcttag agatagaaaa ataaacaggt
    19201 aagcagaaga cggacttttt taatgcgaaa acgtattctt tttattggcc caccgctgta
    19261 cggtttgtta tacccattga tttctctggc tcaggccttt cgtgtaattg gacatgatgt
    19321 agtaattagt agcgctggca aattcgcgaa taaagcagca gaagctggac tggttgtttt
    19381 tgatgcagtt ccaggtttag attcagaggc tggatatcgc catcaggaag agttgaggaa
    19441 aaaaagtaat attattggtc atttctcttt ttttagcgat gaaatggcag ataacctcat
    19501 cgattttgca ggaaaatgga ggccagattt aatagtctat ccccctcttg gtccggcagg
    19561 cccattggtt gctgctaaat atagaattcc ttcagtgatg ctggctgttg gattcgcgca
    19621 tacatctgcc catattcaga tgttaaaccg ttctttaagc aatgcttaca ggcggcatgg
    19681 agtcagcggt ccactatgtg atttagcatg gattgatgtt gctcccccaa gtatgagcat
    19741 tcttaaaaat gctggagaac cggttatctc aatgagatat attccttata acggaggtgc
    19801 tgtaaaggaa acatggtggg acagggattc tgatcggaaa cgtttactta tcagccttgg
    19861 cactgtaaaa ccaatggttg atggtctgga gctgatttca tgggttatgg attctgcaaa
    19921 tgaagttgat gctgatatca ttttgcaact tgcaataaat gctcgtactg gtttacgaaa
    19981 actaccatca aatgtacgtc tggttgactg gatacctatg ggtgtattcc ttaatggagc
    20041 tgatggattt attcatcacg gtggcgcagg taataccctg acagcgctgt atagtggaat
    20101 accacagatt gtgtttggcg aaggtgcaga tcgctctgtt aatgcagaaa ttgttgcgaa
    20161 gcgtgggtgt gggattattc cggacaagca tggactgacc agtgatttgg taaatcgcct
    20221 gctttatgat gattcactac gcttctgttc agatcaggta gccgctgaaa tggctgaaca
    20281 acccagtcct gcagagattg cagaggtttt gatgagaaaa ttaaaaaaca acgggaaact
    20341 attgtagcgt attgctgaaa ttttcaacga gaaatgttat gagtaatata atgtccttca
    20401 tcacataaat gaaggacatc actaaataaa tgagatagtc tcccataata atattgcaga
    20461 aagagttatc ggacgataaa acgatatttt acacagagtt attatttgat accttcctgc
    20521 aattataatt tgctaattca tcaataattg ctccgcgcca ccaggcataa tcgtgaccac
    20581 cggtatagac agtgaggtta ctttccaaac cagctgcgat taacgaatga tgcaatcgct
    20641 gaacatgtga gacggttgta ccttctaaag aaccgactcc aagtcgaata tttacatctt
    20701 tcggaggcgc tgaaagtatc tgctcactta cccaggaggt attattctca gtaaacaaga
    20761 tcgggctgcc ctggtcaggg ttccaccaca ttgaaggtga gtgactaatg attgtaccaa
    20821 atgttgtcga cgcatatata gctgccatca gtgctgtcac tccaccgagg ctctgcccgg
    20881 ccagtacagt attagagcga ccagcccata cgatatccgg atagtctctg tataactggg
    20941 gtatcagatt ttccgctata tcaaagatga gttctttatt gcctcccagt atattcatac
    21001 gatcagattc attaatattg tctatcccca taattgccat tggcgtgata cgaccagtgg
    21061 tgatggccat atctaatgcc cgggtaatat ctatccggtc aaaccatgtt tcagcatcag
    21121 gtaacacaag taatccaaga ggtactgatg ttggattcag gggaaaatat aaacgaatcc
    21181 ggcgttgata tcctgcaaca aaggaatgta atgttgaaag aatacctgta catttatggg
    21241 aagtatcatc ccattccttt tgttcaggag ccatatcaag agaaaggact gattctccga
    21301 atcctcgtat atttatttct gctgttttgt taaatggatc agatttaccg ggtaatggag
    21361 agaagcgact tcctagttga aatatgtctt tttgtgtcat atctgtggga atttctataa
    21421 atgagtatga gccccgatat gaagctggta acaccagtgt cagcatccag atatctgtcg
    21481 aagggatatg agtcattagt ccttttttga cgtctttttt atctgtaaca cgattcaggc
    21541 gaagatatac gccttgcagt gctttctctg agcgccacag aaatgtaacc tcccgatttt
    21601 ctgtagtggt gctttttgtg atcactggaa gtgttgctga ctcaagatag tgccagaaat
    21661 tagcaatatc tgttgttctt attgtgacag gatgtgtcat tttttttaac tgtacacaaa
    21721 tatcttcggg tgtctggaaa agatagtttt tcataggtat catcttaaat taatccaagt
    21781 gtaagaataa tacatcaaat gacgttcatt caagggatga tgagtgactt tccggaatta
    21841 aatctgttgt attgatgata aatataaaac aggtttaata aaatttttgc tttatactgt
    21901 agagttcacg tatgtaagta atatatgaaa aggctctcgt taagatgtct ggtacaaaat
    21961 gacagatcat atgcctttgt aaagagacga cagaagtaga gtcttacagg cacatcataa
    22021 ataccctccg gccatgccgg agggtattta tttaactgcc gctgtttgca ctggatgtgg
    22081 aactactcat actataagaa ggtccacttc cccccggata catgatgatg gatggagtac
    22141 ccggagccca gcatgctcca ttagcatcaa tatatgttcc catatctttt cctcgtagat
    22201 ctattacatt agtagaacta ggtaatccat taagattcat ccctcctttc acgaaatcaa
    22261 gcatgtgatc tgatatttct ctcattttaa aaacctcctg ctcatttatg ttcagatatt
    22321 aatttggtgt tgcaaccatt tatta
//
LOCUS       DAZFFI010000075        21857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFFI010000075 DAZFFI010000000
VERSION     DAZFFI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.075"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHJ12_003302"
     CDS             complement(<1..251)
                     /locus_tag="ACHJ12_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO4991041.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHR"
     gene            complement(402..599)
                     /locus_tag="ACHJ12_003303"
     CDS             complement(402..599)
                     /locus_tag="ACHJ12_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4991042.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(815..1195)
                     /locus_tag="ACHJ12_003304"
     CDS             complement(815..1195)
                     /locus_tag="ACHJ12_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4991043.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(1213..2202)
                     /locus_tag="ACHJ12_003305"
     CDS             complement(1213..2202)
                     /locus_tag="ACHJ12_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4991044.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRLAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(2254..2511)
                     /locus_tag="ACHJ12_003306"
     CDS             complement(2254..2511)
                     /locus_tag="ACHJ12_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991045.1"
                     /translation="MNNRTATVSPEQLRRQAQEMLRCAEQMEKMSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(2508..3908)
                     /locus_tag="ACHJ12_003307"
     CDS             complement(2508..3908)
                     /locus_tag="ACHJ12_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4991046.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAYTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGTPR
                     RYSNNRMSGSKAERLF"
     gene            complement(3905..4783)
                     /locus_tag="ACHJ12_003308"
     CDS             complement(3905..4783)
                     /locus_tag="ACHJ12_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4991047.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSERNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWLDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(4794..5720)
                     /locus_tag="ACHJ12_003309"
     CDS             complement(4794..5720)
                     /locus_tag="ACHJ12_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4991048.1"
                     /translation="MSSKILGNVWDACAAYGIKGAKLMIMARLADYSNDDGVCYPGVE
                     TICRQLGLGESTVRTAISELEADGWLTRQSRRKGNRNTSNLYHLNADRLELLARTERD
                     KVAELKQQRKLSGLRDPSDSEPSKSEPSESVCSGVFDPSDSGKNTRLTLQNLTPDPQG
                     LKHEPPVNSKHEPQDIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFK
                     AWKARIREGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPA
                     APGGGRQRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(5717..5941)
                     /locus_tag="ACHJ12_003310"
     CDS             complement(5717..5941)
                     /locus_tag="ACHJ12_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991049.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGHVLRVCLVNTESQKVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGAV"
     gene            complement(5938..6786)
                     /locus_tag="ACHJ12_003311"
     CDS             complement(5938..6786)
                     /locus_tag="ACHJ12_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4991050.1"
                     /translation="MTRHQHIDSASPGRYTVLAPYKAGAGRENPEFNIEHNRAHAVFS
                     CHEHHYAQIMVGRAGPTSVGPGSMLTGFSPLYVSPPIAVRSLGGEFIEFNIGAVTMTT
                     LPTQSHPEITIINGRVVTTSLAVSNYFTKRHERVLDRIRNLECSAEFTEHNFVLSEYT
                     DASGRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNLMEKSLSGADASDMSAV
                     ARNARGVYLHLREIHQIWTSQLYPMLKAVESPLASKLYDRVGDAVFGAALVDSRLNGS
                     DKEVRP"
     gene            complement(6783..7415)
                     /locus_tag="ACHJ12_003312"
     CDS             complement(6783..7415)
                     /locus_tag="ACHJ12_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4991051.1"
                     /translation="MKFRRHTLHIVAKSTIGSETPELIEATNDAPASFLLSVLAHTRI
                     MVGWAGEPKGSPVSCNAGSLNPVQSATSEIETSGGGTVPLLQEAALMATIPAHPYLKI
                     EVVNGRAVIFSLYVACHFKRMHKNIIDRIEYLNCSREFLTRNFIPGTYRAYGDSVRGY
                     YITRDGLMMLQLGLSLRTMRYYESCIEAFHEAEAVQGHSVFRRNQREVHQ"
     gene            complement(7444..7620)
                     /locus_tag="ACHJ12_003313"
     CDS             complement(7444..7620)
                     /locus_tag="ACHJ12_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4991052.1"
                     /translation="MQVLNRWFRDGRGRRVHVIRWESETQRVIYLRDGYPHECFSPLW
                     LFRRDFVECEAPPT"
     gene            complement(7640..8347)
                     /locus_tag="ACHJ12_003314"
     CDS             complement(7640..8347)
                     /locus_tag="ACHJ12_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4991053.1"
                     /translation="MNTTIFTDKASMTSIEIAELVGSRPDSVKRTIETLAKKGITQFP
                     QTVEIENKQSLGPRRFSSAYVFEGERGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSQIEMVAAMALEAVRQQRRLEQVEEKVTHVTETVEKIKRGTIRDGYAGYRQLVAK
                     TGMSDAKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMSAFHLMMSEAEPRGT
                     RWYHPKMGLFQALGWQV"
     gene            complement(8344..8607)
                     /locus_tag="ACHJ12_003315"
     CDS             complement(8344..8607)
                     /locus_tag="ACHJ12_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4991054.1"
                     /translation="MKNEGIAKAVDIAGSQIALARRCGRAQSTICDWLNGKKKISPEF
                     VPSLVKAVDGKVQAYEFRPDLPELFPHPSLAHTEDGVCEGDKQ"
     gene            8731..9435
                     /locus_tag="ACHJ12_003316"
     CDS             8731..9435
                     /locus_tag="ACHJ12_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4991055.1"
                     /translation="MSFSDRLQLAMTIRGYSQGKLAREVGMAQSSVNKLVNGATGSRK
                     VVEIANTLNVRPEWLSYGVGPMTPEESTSCNPTPQVSYSPLENDKDIFRVEVLDIAAS
                     AGPGTFLVSDFAETVHAIEFSHDAARRLFCSRPANIIKMITVDGDSMAPTLCAGDQVF
                     VDVSVRNFETDGIYIFIFGHTFHIKRLQMQGMQLAVISDNPAYKEWFISESAEEHLFI
                     MGKVLIHQSIQYNRVG"
     gene            9612..10283
                     /locus_tag="ACHJ12_003317"
     CDS             9612..10283
                     /locus_tag="ACHJ12_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4991056.1"
                     /translation="MKHAVKVMDEIAAQIQESGSLLGMIYSYMSENGAESERVYNAIR
                     CLMRSLQKTEETASIFAEKLCEAKAMVSHGHFFGKIRAQREACGLTTTELAKLLDLDE
                     EIILQWESGEFEPTISMLIPLANVIGCDPLSLLDEKDSASVIRVNAPEVYVESIGARI
                     KSARKKLGLTEADLARMINTYSDPINDWECGICEVPADQIVPLASALNCDLMWLLTGK
                     SEAKE"
     gene            10289..10489
                     /locus_tag="ACHJ12_003318"
     CDS             10289..10489
                     /locus_tag="ACHJ12_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991057.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHDLLCAVVVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            10688..10870
                     /locus_tag="ACHJ12_003319"
     CDS             10688..10870
                     /locus_tag="ACHJ12_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991058.1"
                     /translation="MHQKTAEHEQTRVLLTIKNGKVILIRHVHDDEFVGNLSTFLFIA
                     EKAGYDVIAAADEDEE"
     gene            10876..11448
                     /locus_tag="ACHJ12_003320"
     CDS             10876..11448
                     /locus_tag="ACHJ12_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991059.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPSPIRFALQQYIEWNLIQTYGEAEGMGRALFLYQKMLTTSPDKGF
                     ILSDIGREGLELLLDEMINDLNTHGMPEGQ"
     gene            11633..11821
                     /locus_tag="ACHJ12_003321"
     CDS             11633..11821
                     /locus_tag="ACHJ12_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991060.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTRAVISGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            11818..12645
                     /locus_tag="ACHJ12_003322"
     CDS             11818..12645
                     /locus_tag="ACHJ12_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4991061.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQAEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            12686..13057
                     /locus_tag="ACHJ12_003323"
     CDS             12686..13057
                     /locus_tag="ACHJ12_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO4991062.1"
                     /translation="MNTHNAQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVISCAIESLYATLPYEEVEDERTGGTDIMATINIGELICQDEDLSGESW
                     LAGMLVAAEIISIEPATNIRL"
     gene            13089..13331
                     /locus_tag="ACHJ12_003324"
     CDS             13089..13331
                     /locus_tag="ACHJ12_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4991063.1"
                     /translation="MFDLINQGQLYTDSAGYPVKIIHCINNIVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            13335..13469
                     /locus_tag="ACHJ12_003325"
     CDS             13335..13469
                     /locus_tag="ACHJ12_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991064.1"
                     /translation="MNKAFELWVRQCYGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            13488..13742
                     /gene="xisR"
                     /locus_tag="ACHJ12_003326"
     CDS             13488..13742
                     /gene="xisR"
                     /locus_tag="ACHJ12_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4991065.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGVKNASSG"
     gene            13776..15062
                     /locus_tag="ACHJ12_003327"
     CDS             13776..15062
                     /locus_tag="ACHJ12_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4991066.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKKEITIKALSEKWLDLKKIEIGGN
                     AFNRYQSVVRNMLPRIGESRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAIETLKSQAEMTRFGTQYQIDVKLREFGRTVSHECTFVFNPQLVKKCQQAGY
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALAAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELAESVPYMPHRARR"
     gene            15064..15843
                     /gene="yecE"
                     /locus_tag="ACHJ12_003328"
     CDS             15064..15843
                     /gene="yecE"
                     /locus_tag="ACHJ12_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4991067.1"
                     /translation="MISFSIIELTHGSRHVNCVEGNTTLYALPKPEVVLRWREQTTDD
                     FRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALW
                     HFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHAARPHSE
                     AIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYL
                     FLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            15896..16291
                     /gene="yecN"
                     /locus_tag="ACHJ12_003329"
     CDS             15896..16291
                     /gene="yecN"
                     /locus_tag="ACHJ12_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4991068.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            16332..17075
                     /gene="cmoA"
                     /locus_tag="ACHJ12_003330"
     CDS             16332..17075
                     /gene="cmoA"
                     /locus_tag="ACHJ12_003330"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4991069.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            17072..18043
                     /gene="cmoB"
                     /locus_tag="ACHJ12_003331"
     CDS             17072..18043
                     /gene="cmoB"
                     /locus_tag="ACHJ12_003331"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4991070.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLIPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(18209..20638)
                     /gene="torZ"
                     /locus_tag="ACHJ12_003332"
     CDS             complement(18209..20638)
                     /gene="torZ"
                     /locus_tag="ACHJ12_003332"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4991071.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRSSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDATRFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(20663..21763)
                     /gene="torY"
                     /locus_tag="ACHJ12_003333"
     CDS             complement(20663..21763)
                     /gene="torY"
                     /locus_tag="ACHJ12_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4991072.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         5597 a         5586 c         5154 g         5520 t
ORIGIN      
        1 cgatggccac agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc
       61 agacactggt ccagccactt caggtagtca tcgtcgccct tccactggtt atcccagccc
      121 tcaggcttca ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt
      181 aacgaccgga taaattccag gcagtcggcg ttgattaact cacaactgga tatttttaca
      241 gtattaagca tggatcatta agcctgtctc tgataggctc attctgcttt tgcgcaaagc
      301 agtgggcctg aggtttgctt gtgatcctga cgcatgagca gatggctggt gagtgcccct
      361 aacacccacc agccgcccat ttaccacaaa taaaaaagcc ttcaggactg aaggcgtctg
      421 taacaaccga actgatagtc tgccagcccc gccataacaa gctgggtcag tattagctgg
      481 cagcgttcgc gtgaaaggta agtattctgc gcaatctccc cgactgtcgc cggttcggtg
      541 acgcttaatt cattaaacac tgccctggcg gtttcggtca tatcctgctg tttcagcatg
      601 tctttttccc ttttccggtt aacgtgacac accaataact cttgtcgaaa aagccagcaa
      661 gctgaaagac cggtattcac cgccaccagc gcgtttactg tactggcgtg atttcagtca
      721 taaaaaaacc cgctcgcggc gggtttaagc tgtgtggcga agtaaccact cttaacatac
      781 tgacatactt tttgcggacc gcactaatca ttttttactt ttttagcagc cagtcgtcca
      841 tctccagtct tacccccagc acagacaaac atccgtcaat aaacccttcg gctatctgca
      901 tctcaattcg tattgctttt tcgcttttct ttctcgtcct ggctatctgt ctttttgata
      961 ttcgcaacaa ataatgagca atgagaagcg aatactcctc aggttttttc tgcttcagac
     1021 gagcaagaca gttttcaatg ataagtccgt catcatcgca gcaggccgga cgtggtttag
     1081 tggcagatgg taaaagtcct ttgaatccgg cagcgatcgg agaatagtcc accccggtgt
     1141 taccacttgc cgcccatgcc ccccagcgtt caagaaccat ctgaatatca cgcatcaact
     1201 ttctccacaa aatcaggaca gcacaccaat cgccagtgcg cgatcgataa aacgaaatat
     1261 cagctccagt tgggaaccat acttctcttc aaatgccaca gtatccgcat gcagttcgtc
     1321 gtggtgtttt ctgcacaaag gcagcacaaa aaggtcatgc gcttttgtcc ccataccacc
     1381 ctgaccgtga ccaatcagat gatgcggatc atcggctggc tttccacaac acgcgcacgg
     1441 ctgcgtctta acccagcgag tgtacttttc attaacccag cgacgacgtt tcgggcgtaa
     1501 cataaaagac tccggcgact ccggatccac tttcagcgcc agcacctttt tcgctttatc
     1561 ccggatgatg ctggtggcgg gaaccgaagg cacaaggtca ctttcccggg tgacagacgg
     1621 cacaacaggc ttcggtaatc tcagtgcctt acgggctgca ctttccggta aggcatccgc
     1681 caggtcatta cgaaccagcc accagcacag ttccggcatt gtcacaacgt gactgtcatc
     1741 aaaaccaaga tcacggcgca caacagacaa cacccagcgg gcacagttat ccgttgccat
     1801 tgcttccagc cgttccgtga actggtcacg gagaagattg tcacagtgcc agcacagacg
     1861 gattgcaccc ggcgcgagcc gcattgtggt catgttctcg ctgtgccatc cggaatgagg
     1921 ccactgacag cccttttcac gaagtaacca gctttcaaga cattccaccc caccagcacg
     1981 acgaataact gcctcattgc ggaacacggc ccgcacagca ggatcatccg ctaacggttg
     2041 tgatgccgcc ggaacggcac cactggcaaa agatgaataa cgttccggct caggctccag
     2101 caggacacgc ccctgcataa acaggggcat cagctctgag cctggtctga acagtacgat
     2161 ccccattcgc ggggcaattt caggagtcaa cagtgctctc acggtcacct cagcgaacga
     2221 tattgcatga acacaggaga aaaaattcag ccatcacgca gtaaactctt tcaccagcgt
     2281 ttcaaactgg cttacctggc cttccagttc cgccacgcaa tccaccagct catccaccgc
     2341 cttttgtgtg cgatgttttg cctgcagcag atcacgaagc gccggagtaa gctgcttgcg
     2401 gagcgtatcc tttgccacgc tcattttttc catctgttca gcacaacgaa gcatctcctg
     2461 cgcctggcga cgaagttgtt ccggtgaaac agtggctgtt ctgttattca aaataaacgc
     2521 tccgctttac tgcccgacat gcggttattg ctgtatctgc gcggagtgcc cggcgtcatg
     2581 ggagtggaaa gaacccgggc actctcctgg tccacaggca gaaaatgccc gttatgaaaa
     2641 cgccggtaaa tggtacccag cgtgccatta cgctgtttcg tgatgttgat ttcagctatg
     2701 cctctcgcct gtgtctccgg gttgtacacc tcatccctgt aaagcatcag aatgatgtct
     2761 gcatccgcct ctatttcccc tgagtttttc aggtccgagt tcatggggcg tttattgggt
     2821 ctggattcca cgccacggga gagctggctc agagcaatca gcggaaaacc gccggatttt
     2881 gccaggcttt taagtccctt tgagatttcc cccacagcaa ggtcgtgacg ccccgtgctg
     2941 cgggttttaa tcaggccgag gtaatcgacc accaccagcg ccgtttccgg gtgtttcatc
     3001 cggtggtgcc tcgtggttgc acatatctca tcaatggtca ggtttgcctg gtccaccatc
     3061 cagatattac gccccgtcat tcgtcccacg ccctgtgaga aacgtgccca gtcttcgtct
     3121 tcaaaacggg caacagactt aagacgggat accggcattc cccctgcagc agacaccata
     3181 cgttcaccaa tctggatgtt cgccatctcc atggtgaaca gaagcacgcc atgcccctgc
     3241 tcagtcacct tgtcgatgat atccagcgca agttcggttt tccccatcga aggacgagcc
     3301 gcaatgaata ccaagtcgcc tggctccata ccgcccgttt ttgcgtccag ttcatcaata
     3361 ccggtcatca gcgccctgga tttctccagt ccctgattgc ggcattcaac acggtcgacc
     3421 acttccggaa ggacatcatc aatgtgaacc ggctgaatga cgccctttcc ggtcgacagt
     3481 gaggccatca tgttctgcgc atccttcagg gcatcctcgg ctgcttcaca ggtatacgca
     3541 tcacgtaaat tctgtaatgc ttcagtcagt gttttttctg catcgcgcag tgcggcatta
     3601 cgccgcaacg ctgcgacata gtgctccagt gaagacttca cccaggtttt gcgtccggtg
     3661 tcggtaatca ccggggcaag ttccggcatc tcattgcaca gcagtacggg gtcaatgacg
     3721 ccggatacac gggcctgtct gcaaattccc gcgtaaatat cccggtactg tcgtgcagaa
     3781 aaaacgtccg ccgacaatgt ggccagaata tccatcactt ccggatcagc cccacgcaga
     3841 aaaaacgcgc caatgacagc gccttccagg tcatcgttac gccatgccgg ggtgttctgg
     3901 ctggtcatgc ggcaacacct ccgatacgag aacggtagct gggccagtta aacgacaacc
     3961 agttgcgccc gccattggtg atcctgtcgg caatccgggg actgatgaac gcccacaatt
     4021 cttccggtga aaggttgctg atcaagatag ttggcaaaat accctcatac cgggcattga
     4081 taatttcctg caaaatggcc atttcagccg cactgccaaa ctgaacgccg acttcgtcga
     4141 tgatcagcaa atccagtgac gcataatgct caatgacgtc atccgctgtt ttttcactgt
     4201 cattccgcca gcagtttttc acagcacggg taaggcgcat cacgtcggtg atctccacac
     4261 tggccagata gttacggatg atgtgttttg ccattgatac cgccagatga tttttcccgg
     4321 taccgcaact gccggtcata acaagactgg taccgttctc cagcatatct agccagttct
     4381 ccgcatagcg gcgacaggcc gcaagatttc tggctgcgtc aggattaacc tccagataat
     4441 tatcaaactc gcagtcccga aaacgcagag caattccggc gttatcagtc agttcttccg
     4501 ccttgaggga cgacagctcc atggtcaaat cgttggcctc agctatcaag cagtcagggc
     4561 agcatgaaat tttttctctg tcctcgccat tacgctcgct ccacaccaga atatgtgtgt
     4621 ggtattcgcc atgtttttcg caacacccgc gcccttcacg catccagcag gaacgataag
     4681 gccatggttt ttcgcccttc tgagcaaatg caatctctgc ccgtaactca tccatccgcg
     4741 cctgtagtct tgtttgttgt tcacgcaggt taaacgtcat catcgctgtc acctcagaat
     4801 gtcaatttgt cactggattt accgaatttg tcagacatgg ctcccaggcc agccaggaca
     4861 tcgacctgtc gctgtcgccc acctccggga gctgctggct gttgccagaa atcttcgaag
     4921 tgacgatcgg gtccaaagaa cgtcgacgcc tgcttcacga actgggtgcc ggtatttcct
     4981 gagacacgca cccaggcagc atagcgtttc acaccgttga gcatggtttc tggtgtcaca
     5041 ccttccctga ttcgggcttt ccaggctttg aaggctgctg acttggaatt accaccagca
     5101 cgtttgggat attcctgcca ggcctgttca aattccggtg aatattcctg tctggcagaa
     5161 cgcgctggtg cagacgcgtc agcggatgcg ccaatatctt gcggttcatg ttttgaattt
     5221 actggtggtt catgttttaa accttgtgga tctggggtca gattctgaag ggtcaaacgc
     5281 gtatttttgc cagaatctga agggtcaaac acacctgaac atacagattc tgacggttca
     5341 gattttgaag gttcagaatc tgaagggtca cgtaatcctg aaagtttgcg ctgctgtttc
     5401 agttctgcaa ccttatcccg ctcagttctg gcaagcagct caagccgatc agcattcaga
     5461 tgataaagat tggacgtatt acggttacct ttgcggcgtg actgacgcgt cagccagcca
     5521 tcagcctcca gttcggagat tgccgttctg actgtacttt ctcccaatcc aagctgtcga
     5581 catatggttt caacacctgg gtagcacacc ccgtcatcat tcgaataatc cgccaggcgc
     5641 gccataatca tcagttttgc acctttgatg ccatatgctg cacatgcatc ccagacatta
     5701 cctaggattt tgctactcat acggcacctc ccagacgctt aaacattttt ccggactgaa
     5761 acgccaccag cggataactc agggtatgag tacgtccctg aacctggcag acaaccttct
     5821 ggctttctgt attgaccagg caaacccgca gaacgtgtcc gttgctggtg gtgaaccact
     5881 gccccacacg ggggcaatgg ttgtatcggt gatacaggaa attaacgatg tggcggatca
     5941 tggacgcacc tccttgtcag aaccattcag cctggaatca acaagtgcag caccaaaaac
     6001 agcatcacct acacggtcgt acagtttgct ggccagcgga gattcaacgg ccttaagcat
     6061 tggataaagc tggcttgtcc agatttgatg gatttcacgc aaatgcaggt atacgcctct
     6121 ggcgtttcgt gcgacagctg acatatcaga cgcatcggca cctgataaac tcttctccat
     6181 caggttaaag gcgttgatgt atgcctcttt gaaccgggca gcacgtttac cagtgaagcc
     6241 catggcaaga aacgcaaaac cgtcgcgggt gatttgatag cagggaagtt tgcgccctga
     6301 tgcgtcggtg tattcactta acacaaaatt gtgttcagta aattcagcgg aacattcgag
     6361 gtttcgaatt ctatctaaaa cccgctcatg ccgtttagta aagtaattcg aaactgcaag
     6421 agatgtggtg acaacacgac cattgataat cgtgatttca gggtgagatt gggttgggag
     6481 agtagtcatg gtgacagccc ctatgttgaa ttcaatgaac tcaccaccaa ggcttctcac
     6541 ggcaataggt ggtgagacgt acaggggtga gaaaccggtc aacatagaac ccggcccaac
     6601 cgaagttggc cctgcacgcc ccaccataat ttgggcgtaa tgatgctcat gacacgaaaa
     6661 aaccgcatga gcgcggttgt gctctatatt gaattccggg ttctcacgcc cggcacccgc
     6721 tttataaggt gccagaacag tgtaacgtcc cggacttgca gaatcaatat gctggtggcg
     6781 cgtcattgat gcacctcccg ttgattacga cggaacacgg agtgaccctg gacggcttca
     6841 gcctcatgga atgcttcaat gcagctctcg tagtaccgca tggtgcgcag acttaaccca
     6901 agctgtagca tcatcagacc atcacgggtg atgtaataac cacgcactga atcgccatag
     6961 gcgcgataag tgccgggaat aaagttgcgg gtgagaaatt cgcgtgagca gttgagatat
     7021 tcgattcgat cgatgatatt cttatgcatg cgcttaaagt gacaggcgac atacagggag
     7081 aatataacgg ctctgccgtt gacgacctcg attttgaggt atggatgtgc ggggatcgta
     7141 gccattaagg cagcctcctg taacagtgga acagttccac caccggaggt ctcaatctcg
     7201 ctggtggcgg actggacagg gttgagacta ccggcgttac aggaaaccgg cgaacctttc
     7261 ggttcccctg cccagcccac cataattctg gtgtgcgcaa gcactgacaa caaaaaagac
     7321 gctggcgcgt cgtttgtcgc ctcgatcaat tccggggtct cactcccgat agtggatttt
     7381 gccactatat gtaaagtgtg ccgccggaat ttaattttgt caacgaggtt aaatatagag
     7441 tcgctatgtt ggtggcgctt cgcactcaac aaaatcacga cgaaacaacc acagcgggct
     7501 gaagcattca tgcggataac catcacgcag gtaaataacc cgctgtgttt cagactccca
     7561 gcgtataacg tggacgcgac gcccccttcc atcacggaac cagcgattga gtacttgcat
     7621 gtgttacctg tgagcataat tacacctgcc agcccagcgc ctggaacaac cccattttcg
     7681 ggtgatacca gcgagtacct cgtggttctg cttcactcat catgagatga aaagcagaca
     7741 tgaagggttc gactgcaaca atcgctcgac gagacaacaa tccatccggc gtcataaatt
     7801 catgggtatc ggtagggatc tgatatgcgt tcaccagatt gcggcatttc gcatctgaca
     7861 tacccgtttt cgccaccagc tgacggtagc ctgcataacc atcgcgtatg gtgcctcttt
     7921 tgattttctc gacggtttca gtaacgtggg tgactttctc ttccacctgc tcaagacgtc
     7981 tttgttgacg aacggcttca agtgccattg cagcaaccat ttcgatctgg ctcatcggtt
     8041 tacggatttg ttcttccagt tcacgccagc gatctaccaa gcgggcagtg aattcagggc
     8101 aaagctgtgc gacaacaata atgctgtcgc gtttgcctcg ttcaccttcg aatacatacg
     8161 cgctagaaaa tcggcgaggc ccaagtgatt gtttattctc aatttccaca gtctgtggaa
     8221 attgggtgat tcccttttta gccagtgttt caatagttct cttaacacta tctggtctgc
     8281 ttcccaccag ctcagctatc tcaatactgg tcatggatgc tttatcggta aaaattgttg
     8341 tattcactgt ttatctcctt cgcacactcc atcttctgtg tgcgctaaac ttgggtgtgg
     8401 gaaaagctcc ggtagatcag gacgaaattc atacgcctgg actttcccat cgaccgcctt
     8461 aaccagagac ggaacaaatt caggagaaat ttttttcttc ccattcagcc agtcgcaaat
     8521 tgttgactgc gcgcgaccac aacgcctagc cagagcaatc tggcttcccg caatatctac
     8581 agccttggct ataccttcat ttttcatatc gacctccaac ctgattccat tcactatagc
     8641 gattgattaa agcgattgca atgtacggga aagcggtttg tgtatatatc gctaaggcga
     8701 tacactaaaa gggcagaaaa aggaggactt atgagctttt cagacaggct acagttagct
     8761 atgactatcc gcggatactc gcagggaaaa ctggccagag aggttggcat ggctcaatcg
     8821 agcgtaaata aactggttaa tggtgccaca ggctccagga aagttgtgga gatagccaat
     8881 accctgaacg tgcgccccga gtggctatcg tacggtgttg gtcctatgac cccagaagaa
     8941 agcacatcct gcaatccgac accacaggta tcatattccc cgctggaaaa tgataaggat
     9001 atttttcgtg tggaagtcct ggatatcgct gccagcgcgg gaccaggaac attccttgtt
     9061 tcagattttg cagaaacggt ccatgctata gaattttcac atgacgccgc aagacgtctg
     9121 ttctgtagca ggccagcaaa tatcataaag atgataacag ttgatggcga cagcatggct
     9181 cctactctgt gtgcaggaga tcaggtgttt gtcgacgtat ccgtcagaaa ttttgaaact
     9241 gacggtatct atatttttat ttttgggcac actttccata tcaaacgcct gcaaatgcaa
     9301 gggatgcaac ttgctgttat atcagacaat ccggcctaca aggaatggtt tataagtgaa
     9361 tcagctgaag aacatctatt cataatgggg aaagttctca ttcatcagtc aatccagtat
     9421 aacagggtcg gataagtcaa catcaccggc agctgtattg cgcagctgct ctactttcct
     9481 gtatctcaac tccctacgta tcgtctcatt tctctcataa tctgttcttt gctgcccaaa
     9541 atgcatagct atagcgattg acataaccaa acgctttggc tattgtaaaa ttaaaacaac
     9601 aggaggctca tatgaagcat gcagttaagg tgatggatga aattgcagca caaatacaag
     9661 aaagcggctc gctgcttgga atgatctatt cctacatgtc agaaaacgga gcagaaagtg
     9721 agagggttta taatgcaatc agatgcctga tgcgttccct gcaaaaaaca gaagaaactg
     9781 catcaatttt tgcagaaaaa ctatgtgagg caaaagctat ggtttcgcat ggtcactttt
     9841 ttggaaaaat ccgtgcccaa cgtgaagcct gcggcttgac gaccaccgaa cttgccaaac
     9901 tgcttgatct cgatgaagag atcatcctgc aatgggagag cggagagttc gaaccgacca
     9961 tcagcatgct tatccccctg gcaaacgtca ttgggtgcga tccgctttct ttgctggatg
    10021 aaaaagatag cgcgtctgtt attcgcgtaa atgcgcctga agtctacgtg gaaagtattg
    10081 gcgcacgcat caaaagcgca cgtaaaaaac tgggcttaac ggaagctgat cttgcccgca
    10141 tgattaatac ctatagcgac cccataaacg actgggaatg cggcatctgt gaagttcctg
    10201 ctgatcagat agtgccactg gccagtgcgc ttaattgtga cctgatgtgg ttgttaacgg
    10261 gaaaatcaga agcaaaggag tagcaacaat gactggtaat atccatgata agtatgaagg
    10321 cttatgccta gcaccagatt ccttcgcaaa caatatccat gatttattat gcgcagttgt
    10381 cgtattacaa atgtcagaca acgacgcaat aaaaagaaca ggtgatgaag ttcttgaatt
    10441 tgcacgttgc tatgctgaag cagcagctga aaaagaacta tccagttaaa tagaacaagt
    10501 catctccaga tactatatta cggcttaatc gccggggatt atcacacact taatccacag
    10561 gaggttttat tatgactttt ataaaacata aggcatcaca caaaacagcc tgccttattg
    10621 cacaacacgg gaaaaattac atgcatattg cctgcttgtt tctgcgtaaa gcatacggga
    10681 gataataatg catcagaaaa cagcagaaca cgaacaaacc agagtattgc tgaccatcaa
    10741 aaacgggaaa gtaatattaa ttcgtcatgt tcatgacgat gaatttgtag gaaatctttc
    10801 aacattcctg tttattgcag aaaaggcagg atatgacgtt attgcagcag cagatgaaga
    10861 tgaggaataa atttcatgca atacgctgaa ttccaggctg aagcaacagc caatggtata
    10921 caaacaggca gtatgactat tgattatcac gacgccatac gccgtctgga tgctggagaa
    10981 ttcgatactc ctaatgtgcg aggtttacgt atccttcagt gtctcgcgca agctgacgaa
    11041 gcaggattac tgggaaaact tccggttgag atgaaggttg cccagtggcg atggttgtat
    11101 gtgacgacat tcatcaacga agaagaagac aagaacggca caattgatgt cccgaatgaa
    11161 cacggaacaa cagatcgcgc cgtaatatat aacgggaagc atggagttat gactatatac
    11221 cctagtccca ttcggtttgc cttacagcag tatattgaat ggaatttaat tcaaacatat
    11281 ggcgaagctg agggaatggg aagagcgctg tttctttatc agaaaatgct caccacttcc
    11341 cctgataaag gtttcatcct ttcagatata ggtcgagaag ggcttgaact ccttctggat
    11401 gaaatgatta acgacctgaa tactcatggt atgccagaag gccagtgaca cattaaatat
    11461 taagaagaat ataactcttc cgttttttac taaccgttta tatgaaaagc gaccgtgaat
    11521 taagcagagt aaaactgctt ttaatccttg ccacagtact gacactaaca gaaatcatta
    11581 ttctctttat tgcgctgtct gtcagttaaa aatatcggga taccacatac caatgagact
    11641 gtatttcaca atagtaattt tactggcaat tatcgcatgc atttacggat tactcgttcc
    11701 gttccttata tccatgaagg atacgagagc agttatttct ggctttgcac tggcgtttct
    11761 gaccccgccc tgcatttatg ccatttacaa gggtctttct ttcactaagg ataaaaaatg
    11821 aaaaaaatta tttttgcttt agctattgtt ctgccgacca ttggtcttgt cggttgcgat
    11881 cgcgttgagc ccggtaatgt tggcatcaaa gtaaataaac tgggcgacga taaaggcgtc
    11941 ggtgaggtgg tcggtgttgg tcgctactgg actggctgga atactgaagt ttacatcttc
    12001 cccaccttca agcaaatgaa gacctacgat gaaccgttca gctttcagat gagtgacggc
    12061 acaaccatcg gctatcacat cggtgtggcc tacaaggttg atccatccaa agttactaca
    12121 gtgtttcaaa cctaccgcaa aggcgtggat gacattaccg acactgacct gcgccagaag
    12181 atagccgacg cactcaaccg actggccagc aaaatgacca ccgataaatt tatcgacggt
    12241 ggcaagtctg aactgctgga ttcagcactt aaagatattc aggcagaaat gacacccatc
    12301 ggcattcagg taatgagcct ctcttatgtc ggtaaaccgg aatacccgcc aaccgtgatc
    12361 gacagcatta atgccaaagt cacagcgaac cagaaaaccc tgcaacgcga acaggaagta
    12421 aaacagcgcg aagcggaagc caacatgttg cgcgcggaag cagccggaca ggctgacgcc
    12481 attcgcacaa aagcccaggc cgaagccgat gctattcgtt tacgcggtga agctctgcgc
    12541 cagaaccccg gtgttatgga gctggaagcc atcaataaat ggaacggcac actgccgcaa
    12601 tacatgacca gcggtgccaa tacaccattt atccaggtta aataacttat atgcccggca
    12661 ggccgccggg ctaagggaaa agcagatgaa cacccataat gcccaaccgc aaataatgaa
    12721 ctatgacccg aatctgacgt catgcggacg catggcaaaa caaaccgttc gattaacttt
    12781 cgggttatgg gaataccgcg aaacattcga agttactgtc ggcggcaatc tgaccggact
    12841 ggatgttatc agttgcgcta ttgaaagcct gtacgcaaca ctgccttatg aagaagtcga
    12901 ggatgagcgc acagggggaa cagatatcat ggccaccatt aatattggcg aactgatatg
    12961 tcaggatgaa gacctgtccg gagagagctg gcttgctgga atgcttgtag ctgcagaaat
    13021 tatcagtatt gaacccgcta caaatatacg gctctgaagt tctcgctatt cagagaacag
    13081 gagaaaaaat gttcgatttg attaatcaag gacaactgta taccgacagc gccggttacc
    13141 cggtaaaaat tattcactgc ataaataaca tcgtgttgta cagaagaatg gatggacgaa
    13201 cacagtcggt aaaaataaac gattttaatg aactgtttga acgaatcgat caccaggaat
    13261 accgacaaat tctggcagaa acagaacaag aagctcatct gaaaaaatta cgtgccatga
    13321 aaaggaagta aagaatgaat aaagcgtttg agctatgggt acgccagtgt tacggcaatc
    13381 gctatgacct gacgcgagat gttgacggtt tctactgccg tgaagttgtg aaacgaatgt
    13441 ttgacgtgtg gtgccactgc cgtggatgaa aattttatga ggttggcatg cagacaatca
    13501 tctatcagat aacccccagc aaatggtgta cggagagagt cctcattgca tcaacagggc
    13561 taaagcctgg caccattgag cgggcaagaa gaaagtcatg gatgcaggga aaagaatacc
    13621 gccattacgc tgtagaaggt gatccggggc actacagtga atgcctgtac aacatcgaag
    13681 aaattatgcg atggatcgaa aaccagaaac aaccaggtgt caaaaatgca agttccggtt
    13741 aacctgttaa tgctcctgga cgtctgggag gttcaatgag taacacatca tacccgacag
    13801 gcgttgaaaa ccatggcgga tcactccgca tatggtttca ctataacggc aaacgtgtca
    13861 gagaaaacct cggtgttcct gacacagcca aaaaccggaa gattgcaggc gaactcagga
    13921 cttcagtttg ttttgcaatc agaatgggga atttcgacta cgctgcgcag tttcctgagt
    13981 ccccaaatct gaaacacttt ggtctgggaa aaaaggagat aaccattaag gcgctttcag
    14041 aaaaatggct ggatctgaaa aaaatcgaaa taggaggtaa cgcattcaat cggtatcaat
    14101 ctgtggtgag aaacatgttg cctcgtatag gggaaagccg actggcctca tctgtaacaa
    14161 aagaagattt actcttcatc aggaaagatt tattgaccgg gtatcacaat ctctcaaacg
    14221 gcaaaacaac acctgttaag gggagatctg tagttacagt taattactac atgacgacaa
    14281 ttgcaggtat gtttcaattt gcagctgaca acggctacac ggaatcaaac ccgtttaacg
    14341 gcctgacacc attaaaaagg tccagaaccg agccagatcc gctcacgcat gacgaattca
    14401 ttcgttttat tgatgcttgc caccatcaac aaacgaaaaa cctgtggtct ttagcagtat
    14461 acacaggcat ccgtcacggt gagctaatat ctctcgcctg ggaggatatt gatttaaaag
    14521 ctaaaacaat gactatccgt cgtaattata caaaactcgg ggagttcact ctaccaaaaa
    14581 ccgaagcggg aacagatcgt gttattcatc ttgttcaacc agccattgaa accctgaaaa
    14641 gtcaggctga aatgacaaga tttggcactc aataccagat tgacgtcaaa ctgcgggagt
    14701 tcggacgcac cgtaagccat gaatgcacgt ttgtctttaa tccgcaactg gtgaaaaagt
    14761 gccagcaagc cggttaccac tacaaagcgg actccatcag ggattcatgg gcatcagccc
    14821 tgagacgggc cggactacgg cacaggaaag cctatcagtc aaggcatact tatgcctgct
    14881 gggcactggc cgcaggagcg aatccaagct ttatcgcaaa ccagatgggg cacgcaaacg
    14941 ctcagatggt attcaatgtt tacggagcat ggatgaaaga taacaatatc gggcagatag
    15001 aactgctcaa taaggagtta gctgaaagtg tcccatacat gccccacaga gcaagacgct
    15061 gaaataattt ctttcagtat cattgagtta acccatggtt cccgccatgt taactgcgtg
    15121 gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg ctggcgtgaa
    15181 cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc gcatcaggca
    15241 gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc accgttggct
    15301 ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg ggagctgcct
    15361 gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt ggaagtccgc
    15421 catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg tttacatcag
    15481 cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcagcacg tccacacagt
    15541 gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
    15601 gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
    15661 tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
    15721 ttacatacgc cagacattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
    15781 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
    15841 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
    15901 aagcgcgctg tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
    15961 tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg aactgcaaag
    16021 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgctgatgct
    16081 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
    16141 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgttccgct ggcgacgttc
    16201 cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
    16261 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
    16321 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
    16381 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
    16441 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
    16501 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca
    16561 acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg atgattgaac
    16621 gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc attgaaggtg
    16681 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
    16741 tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga ctgaacccgg
    16801 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgt
    16861 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
    16921 agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
    16981 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
    17041 cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact tttattctct
    17101 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga ttgctaactg
    17161 gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg aatttctgcc
    17221 tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa gcgaagagcc
    17281 actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga tgccatggcg
    17341 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
    17401 atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc tggatgtcgg
    17461 ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg
    17521 gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
    17581 cgatcagcgc gcacatttga taccgttagg tattgaacag cttccggcac tgaaagcctt
    17641 tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg agcatctctg
    17701 gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc tggttattga
    17761 tggtgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc gtaatgtcta
    17821 tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat
    17881 ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg aatggatggt
    17941 taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag ttgaaggtta
    18001 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg gtattactgc
    18061 cggatgcggc gtgaatgcct tatccggcct acaaaccctt gctaattcaa tctattgcag
    18121 ggactatgta gatactgata agcatagcgc atcaggcagt tttacgtttg cataacctca
    18181 gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg
    18241 ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac
    18301 aggcatttgc cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt
    18361 tacataagcc attttccaga tctggccatg caccttcatg cacgcatacc acgccttttt
    18421 tgatcccgtc agtcaccacc gcacctgtta gtatctgacc acgtttgttc cagacacgca
    18481 ccagatcgcc attcgcaata ccaaatcgag tagcatcttc ggtgtgaata gtgatcggtt
    18541 cacgatcggc aaccgcatat tttttacgca gtcccgcata gttaagctga ctgtgtaaac
    18601 ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact
    18661 catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg
    18721 ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc accggatcgg
    18781 cgcggaaatc accataacga acgtactgtt cgttcttctc gctgctgcgc atttcgatca
    18841 gtttattttg ctgccagaag gcattaaaca tcggcatagt cacgcgttgc gcacgagcac
    18901 ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat
    18961 agatctcttt tccgccaggt ttcagtaatt cagcaagatc ggcaaatacg tcaaaatcgt
    19021 tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt
    19081 aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat
    19141 ctgcatgttt agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct
    19201 gccatgcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc
    19261 accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga tatttgccac
    19321 caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt ccgccatcat
    19381 cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa
    19441 cacgcgtcgg attgccaccg ttggagtaat gataagagaa accaaaaccg ccgcccggtg
    19501 taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat
    19561 attgctggcg ctgaattccc cagccagcca tcagcattgt gcgattcgcc gccatcagtt
    19621 cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc
    19681 tcttcggagt attatcgctc ttacctgtca gatactcttc aaactgcgga tacccggtgg
    19741 tgtatttttc aaggaacact ttatcgtgtt taccttgtgt catcagggta tgcgcaatcc
    19801 ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcaccaa
    19861 agaactcgat ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt
    19921 tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg
    19981 tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga atcagcggcc
    20041 aggaggtctg ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg
    20101 ccgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac
    20161 gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg
    20221 caaaaatagc cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt
    20281 ttaatgcttg ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt
    20341 gcagtggatt atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt
    20401 gtacctgatc cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg
    20461 ttgaagaaac aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg
    20521 tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc gatgtcacca
    20581 ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt aatgtcataa
    20641 cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga
    20701 aataccgcgt taaaatgtcc agttcgtttt cgctcatgct ggttcgcgca cccattcctt
    20761 tggcaatgga cggccatgca ttgacggtgt aatggtcagc ggcaataggc gcatggcaac
    20821 cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta cggtccgcta
    20881 atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt
    20941 cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt aatgtggcta
    21001 gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc atttgataac
    21061 cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga tcggtaccag
    21121 gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata tgtgcgccat
    21181 tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta
    21241 tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg ccgccctttt
    21301 gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc atggcatcaa
    21361 aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc catactgttt
    21421 cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg ccgctaacaa
    21481 attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact
    21541 ttggaatatg gcaatctgca cattccgcac gtatcccttt ctggttcgaa aagtggacag
    21601 ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgctgtat
    21661 ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg
    21721 ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaaataaa
    21781 ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg cttgcataac
    21841 tcggcacaac actgcct
//
LOCUS       DAZFFI010000076        21499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFFI010000076 DAZFFI010000000
VERSION     DAZFFI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.076"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /locus_tag="ACHJ12_003334"
     CDS             <1..351
                     /locus_tag="ACHJ12_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4991073.1"
                     /translation="YLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQ
                     RRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQ
                     EILSEFPQPVSLAG"
     gene            complement(394..1545)
                     /gene="ydiO"
                     /locus_tag="ACHJ12_003335"
     CDS             complement(394..1545)
                     /gene="ydiO"
                     /locus_tag="ACHJ12_003335"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4991074.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1559..3154)
                     /gene="ydiF"
                     /locus_tag="ACHJ12_003336"
     CDS             complement(1559..3154)
                     /gene="ydiF"
                     /locus_tag="ACHJ12_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO4991075.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3298..4056)
                     /gene="aroD"
                     /locus_tag="ACHJ12_003337"
     CDS             complement(3298..4056)
                     /gene="aroD"
                     /locus_tag="ACHJ12_003337"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4991076.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4087..4953)
                     /gene="ydiB"
                     /locus_tag="ACHJ12_003338"
     CDS             complement(4087..4953)
                     /gene="ydiB"
                     /locus_tag="ACHJ12_003338"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4991077.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4965..6230)
                     /gene="ydiN"
                     /locus_tag="ACHJ12_003339"
     CDS             complement(4965..6230)
                     /gene="ydiN"
                     /locus_tag="ACHJ12_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4991078.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSTLATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6457..7671)
                     /gene="ydiM"
                     /locus_tag="ACHJ12_003340"
     CDS             complement(6457..7671)
                     /gene="ydiM"
                     /locus_tag="ACHJ12_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4991079.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HQSLKENVAPGG"
     gene            complement(7771..8127)
                     /gene="ydiL"
                     /locus_tag="ACHJ12_003341"
     CDS             complement(7771..8127)
                     /gene="ydiL"
                     /locus_tag="ACHJ12_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4991080.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8263..8370)
                     /gene="rprA"
                     /locus_tag="ACHJ12_003342"
     ncRNA           complement(8263..8370)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ12_003342"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8556..9668)
                     /gene="ydiK"
                     /locus_tag="ACHJ12_003343"
     CDS             complement(8556..9668)
                     /gene="ydiK"
                     /locus_tag="ACHJ12_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4991081.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10057..13113
                     /gene="ydiJ"
                     /locus_tag="ACHJ12_003344"
     CDS             10057..13113
                     /gene="ydiJ"
                     /locus_tag="ACHJ12_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4991082.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEEKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13110..13520
                     /gene="menI"
                     /locus_tag="ACHJ12_003345"
     CDS             13110..13520
                     /gene="menI"
                     /locus_tag="ACHJ12_003345"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4991083.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13620..13808
                     /gene="ydiH"
                     /locus_tag="ACHJ12_003346"
     CDS             13620..13808
                     /gene="ydiH"
                     /locus_tag="ACHJ12_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4991084.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14357..14725
                     /gene="sufA"
                     /locus_tag="ACHJ12_003347"
     CDS             14357..14725
                     /gene="sufA"
                     /locus_tag="ACHJ12_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4991085.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14734..16221
                     /gene="sufB"
                     /locus_tag="ACHJ12_003348"
     CDS             14734..16221
                     /gene="sufB"
                     /locus_tag="ACHJ12_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4991086.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16231..16977
                     /gene="sufC"
                     /locus_tag="ACHJ12_003349"
     CDS             16231..16977
                     /gene="sufC"
                     /locus_tag="ACHJ12_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4991087.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16952..18223
                     /gene="sufD"
                     /locus_tag="ACHJ12_003350"
     CDS             16952..18223
                     /gene="sufD"
                     /locus_tag="ACHJ12_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4991088.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAQLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18220..19440
                     /gene="sufS"
                     /locus_tag="ACHJ12_003351"
     CDS             18220..19440
                     /gene="sufS"
                     /locus_tag="ACHJ12_003351"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4991089.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19453..19869
                     /gene="sufE"
                     /locus_tag="ACHJ12_003352"
     CDS             19453..19869
                     /gene="sufE"
                     /locus_tag="ACHJ12_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4991090.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20018..21022
                     /gene="ldtE"
                     /locus_tag="ACHJ12_003353"
     CDS             20018..21022
                     /gene="ldtE"
                     /locus_tag="ACHJ12_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4991091.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21086..21322)
                     /gene="lpp"
                     /locus_tag="ACHJ12_003354"
     CDS             complement(21086..21322)
                     /gene="lpp"
                     /locus_tag="ACHJ12_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4991092.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5592 a         5235 c         5440 g         5232 t
ORIGIN      
        1 tatctgaata ataattatcg cgaaaaaatc actttagaac agttatcgaa aaaatttcgt
       61 gccagcgtca gttatatttg ccatgaattt accaaagagt atcgtatttc ccctattaac
      121 tatgttattc aacggcgtat gacggaagcg aaatggtcac tcactaatac tgaattatca
      181 caggcagaga tctcctggcg tgtgggttat gaaaatgtcg atcactttgc caaactgttt
      241 ttgcgccatg tcggttgttc gcccagcgat taccgcaggc aatttaaaaa ctgttttgcg
      301 gaacaagaaa tcctatctga atttcctcaa ccggtaagtc ttgccgggta aatctggcgg
      361 cgcagtttgt taagaagctg cgccgcctgc agattatttg ttctgataat ctttcaggat
      421 ctgccgaccc gcaacgtaaa tcataatttc gtctgtaccg ccgccgatac gttcacaacg
      481 gacatcacgc cagaagcggg agacgcgcgc ctcatcggta tagcccagac cgcccatgat
      541 ttgaatcgca tcatcaatga cttccattgc ggtacgtgcg caatacagtt ttgccagcgc
      601 cgcgctggta cgcagtgact gatgctgatc ggcttgccat gccactttca gcaccatgtt
      661 gcgcatgttg tcgatcttaa tcgccatcag cgccagtttt tcctggatca tctggttatg
      721 accaatgggc ttaccaaaag cgatacgttg gttggcatag cgggcggcat cttcaaaggc
      781 gcattcggca aagccggtgc tgcgcgcggc gttgatcagg cgctccatct caaagttgta
      841 catcacattg aggaaaccca ttccttcttc gcccaccatg tcgctctctt caacttccac
      901 attgtcgaga tagacttcgc aggtgctgag catatgccag ccgattttat gcagcgggtt
      961 aatcttaatg ccgggcttac tggagtcgac ccaccacagg gtgaaggctt ttttgggatc
     1021 tttcggttgc ggatcgcgcg ccagcaccag catatacggg tactctttcg caccggtaat
     1081 aaaggttttt tgtccgttga tgtagacctt gccgttttta cgcgtgtaag tggtagtggc
     1141 actgttgtta tctgagccag cacctggttc cgtcaacgcc agggcatagg cgggatcacc
     1201 cgtttccagg gtgctctctg ccgttttacg tagctgctct gcagaaccga aacggcgcat
     1261 actgtgaata cattgaccgt tggtaattaa aaatgccgga gcaccgcatt ttgacacttc
     1321 catcagcgcc agcatttggg tgacgtaatc cgcagggata ccaccaaatt cttccggcac
     1381 gccaagcatg gaaataccgt tatccgccag cgcccgcata aactcacgcg gatatgtccc
     1441 gttttgatcg caggtgcgga aatactcttc cggaaagtta gtcgtaatca gttcgcgaat
     1501 actggccagc agcagttctt gttcttcagt taaagaaaaa tccatcatta tactcctgtt
     1561 aatgagccgc ttcaggcagg acaaaaccca tcgccgcatc gataaataat ctttcgtcca
     1621 tcagtttgag ttctggcgaa atcactgggg tgaagtccat tttgtcgaga atatcttttt
     1681 gtaaatcgac gccaggggcg atttcgatta aatgcaggcc gtcttctttc agcgtgaata
     1741 cagcacgttc ggtgatgtaa cgaacatcca gtccccgttc gagcgcgatt tttccgctaa
     1801 aagtaatttc cggtagttcc cgaataaatt tatttacccg tccttcctgg acgatatgca
     1861 atttgccatc ggcaatttct gtttttaaac tgcccgcagt taacgtgccg cagaaaataa
     1921 ttttcttcga ggtggcactg atatcaataa atccaccggt acccatgatt ttgccattga
     1981 atttatgcac gccgacgtta ccgtgctggt cgacttcagc aaaactcaaa taacaaacat
     2041 ccagaccacc accgtgataa aaatcaaact gggacgtcat atccagaatg gcgcgggtat
     2101 tcacgttcgc gccaaaggcg ataccctgtg aagtaattcc gccaatcgga cctgtttcta
     2161 ccgtcagaat aaagtcatca gcacaacctt cttcccgggc gaccaggcca ataccgtcag
     2221 caataccgac gccgacattc cccaccgcgc ctttacgcat ttcgaataat gcacgccgcg
     2281 caactaattt acgttggttt aggggcaggc taagtttggt gctgtcatca agggtgaagt
     2341 caccagaaat aaaacggtta accggcgcac cgccatacag ttggctttga tccggatcga
     2401 ccaccacaat atccaccaga taacccggaa tacggacaga tttaggatgc agcgtggctt
     2461 tcttaaccat tttctgcacc tgcatcatca caataccgcc gttattgtgc accgcctggg
     2521 caataaccaa tgcgtcgaga tacatcacct catcttcaaa agtggcgtag ccttcactgt
     2581 cgcaggtggt agcgcgaatg aaggcgatat ctggcgcaat cgctttgtaa tagagatatt
     2641 ctttgttatc aaactcgacc agtttaatca ggtcttcttt cgtgacttca ttcagtttgc
     2701 cgccttgctg gcgtggatcg acaaatgtcc cgatgccaat atcactaata ataccaggct
     2761 ggtgggctgc ggcggcgcgt aaggtttgtg taagtacacc ttgtggatag ttataagcaa
     2821 taattttatt ttgttctgcg agttcagaaa tacgcggcga ttgtccccag tgaccacata
     2881 atgcccattt caccagacct tcttgcgcca gaggactaat accgcggtcg gcgcgatcgc
     2941 caagccctgt tggactaata atcgataaat tacgtggcgt ttgagtctgt ttatatttat
     3001 cagcaagagc agtaattaac gtggtggctt ccagaatacc gccgccagcg cctaacacac
     3061 aaagtgttgc ttcgtcggga atataattca ccgcttcctg tgccgacagg accggcactc
     3121 gtccattaat acgaggtggt tttacaggtt tcattgttaa ttcctgattc agatgtgatt
     3181 ttccctacgc cagatggaga attagcgcac agagactcac gaaatatttt tattaaatac
     3241 ggttaaatat attttttaag ttcggcgggg agggtgttcc cgccgaaata ttattgctta
     3301 tgcctggtgt aaaatagtta ataccgtgcg taaatcattt accgagattt gccctggtgc
     3361 agaggctttt tttaccgcac caaaagttgc cgccgagcca aatacttcac cagccagacg
     3421 agaaattacg ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg catactgctc
     3481 ctgcatctcc agggtcgcgg taagcaacgt cagcacatcg ctggtacttt gcggcatcag
     3541 cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg caatgatttc
     3601 ttcggcttcc ggcgttttat ggaagtcatg gttggacatg acaactttca catcatgcgc
     3661 gtgggcgtag gcgacggttt ctttaacctg atcatcgccg gtaaataact ccagatcgat
     3721 catatcaacc aggccgctgt cgatggctgc acgattgaga gcaatataag cctcggtgga
     3781 aatcgcctgc tcgccgcctt ctttggcact gcggaaggta aacagcagcg gtttttctgg
     3841 catggtttca cggagaattt ttgccgccgc catgacagac tccacattgg agaggtcggc
     3901 aaagtggtcc acacgccatt ccagaatatc aaagtccgct tcacgatagg cgagagcttc
     3961 ggatttcacg cgggcgatat ctttcgccat cagcgagacg atgattttag gtgcgcccgt
     4021 accaatgacg agatctttta cagttacggt tttcattttt taccctttct gcacgcggtc
     4081 agcctgtcag gcaccgaacc ccatgacctg tttaacatat tccagaggga aatctttgcc
     4141 agtccataac gtgaactgtt cagccccttg ccacaacaac atgccgtatc catcaatcgt
     4201 tttgcaacca gcttgttgcg cctgctgcaa taacttcgtc atatgcgggt tatacacgca
     4261 ttcagtgacc agcagtcccg gatgtaacag actgatatca ttaactaatg attcattctc
     4321 aaggggtttc atacccactt ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc
     4381 agcaaaggct tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg tgttttcgtt
     4441 aacccgctgc gcgaaggcga gggctttatc gaagaactca tcccgacggt taaagagttt
     4501 aatttctttt aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg cacctccggc
     4561 ccctaacagc accattgttt tgcctttgat atcaaaaccg ctctctttaa tggctcgaat
     4621 atggcccgtg ccgtcggtgt tatagccgcg caggtaacca tcatcattaa cgatggtgtt
     4681 gatggcaccc accagtttgg ctgctggcgt taattcatca acatattcac acgccagttg
     4741 tttgttcggc attgatatac cagttccgcg cattttgagg gcttttaatc cttcaattgc
     4801 tccgggaaaa ctatcgttat ccacttcaaa ggccatatag gtaaatggca atcccgcttt
     4861 ttctaaggct ttattctgca tttcaggcga taaactgtgg cggataggat aggccatcag
     4921 cccaatcaat tcgtattttg cggtaacatc cataattaac tcctttaacc tctatgctta
     4981 attgtgttta atcgggtgct aaatttgcgc tcgccaaacc gcacatcatt ctcaggaata
     5041 ataaaaacgc ggtaatagcg gataaaaaca ataattgcgg taatcagggc cagcagcgcg
     5101 aaagtaaaat cgagaacaat gatatattgc aggccgatgt tcgacagata accggtaatc
     5161 agcggaataa caaagttagc cagtccaccc atcatcatat aaatactggt gactttggct
     5221 ttgctttttg gaaaaaactc tgacatgacc gaaacaccga gctgtaaaat gccgccagct
     5281 gctgagaaac cgataacaaa ggcgccggca ttacacacca gtggagaagg gtacaggtag
     5341 ataatggctg ctgttattgt tgccagtgta gagttaaata cattagccca gatgggccgg
     5401 accatttttt tcagtagtgc ggcaaaaata aagacacaga ccaacgagcc catactgtaa
     5461 taagagatgg tttttaatgc ctcagcttct gacataccag caaaagccat cgcatatttg
     5521 ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat
     5581 gaaacacctt ccagccagac taacggtttg ctgttcattt gcggtaattc attagctacg
     5641 ctggcgtcca ccaactggct ggggaatttg cttttcaaca gcatcagcgt gatcagtaca
     5701 aataggatgc ctggaataat cagcccatag ccgtaccaga tgttattgag caacatatag
     5761 ctcaccagca ttgggtagaa catttgtccg aatgacacca tcgctttaac cagtatgacc
     5821 gccgaaccag aggctttcgg aaagcattcc atgagcgcgg ggtagccacc cgtatccagc
     5881 gctgagttag cgatacctac gcacactgcc agaccgtagg cgagagttaa attcgggcaa
     5941 gcgggaatac caaagaagaa tagcagatac attattactg ccattaatat caccgcccga
     6001 cgaccaaact tatcggagat cacaccgaag aataaaatac tgatcaatcg ccccaaaccg
     6061 ataccggaaa ttaaataggc aatgcccgcg ttgtcagtgg aaaacttttc cgccagagat
     6121 gacatgtttt gggcaagcgt aataacacta atgccgtgca ggaagtagct gaagtaaata
     6181 caaagaacag ccaggataaa tggcgtgctg aaagccttat tttgagacat tttcacagct
     6241 ccttgctgaa agataatttc gttaccgtac gtattgttct ccgacggttt acataattac
     6301 tatactgaag atgattttcc ctgggtacat ggcataaagt ttatgtttcc aggaatattg
     6361 catgtcatag tttgtttttg atcgcgattt tccttagtta gcgcagaaag agttatttat
     6421 ccgtaagatc ttgcatgacc tgaaaagaat attgcattac ccacccggag cgacattttc
     6481 ctttagcgac tgatgccgcg tttttttgcg gctgcgtaag ccgataaaca gtgccagtaa
     6541 aaagccgatg gcagcgatgg cggtatcgaa ccacataata tcggcaatac ttctttggga
     6601 cagatgagcc gtaatcaacg gaatagtaaa ggtcgcaata ctgcccgcac tgtaatagat
     6661 ccctgtagct ttacctttag cgtaggggaa acgttcagcc attaacgtca ggccaatttg
     6721 cacaacacct ccagcagagg taaaaccaat gacaaaagca aatattatca ccacataaaa
     6781 tgtgggatgc aggcagacgg taaacagagc aataaatgag ataaaggtgt acagcatcag
     6841 taatgttgtt gggcgaacgg tatttcgaat gagtggagcg gtaataaata cacaaagcag
     6901 cgaacccacg gtgtagatac tgagtagttt gatcgacata gtgtatgaca tgcctgcaac
     6961 aaattgtccg tactgtgaca gccactggct aaccagataa aacgttgcca ttgagatata
     7021 gccatataag gtataactgg ctaaatcgat aattgaacag cgatgttccg tagagcgggt
     7081 ggttttcttt atgacaggta agcgacgacc cggatggggt gggaacgtac aacggtataa
     7141 aaacagagcg ttaataagca taatgcctgc agcaatcata aaggaccaac cgaaccacag
     7201 ttcagcccac actaacaggc taatgattag cggtaataaa aattgtccgc tggaaacaaa
     7261 tgctttaatt aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg
     7321 ataagtgcct gcatcgagaa aactgtttgc cattcccgcc agaaagccaa aaacataagc
     7381 gataatgatg ttattggtct gcaggatgcc aaaaaagaag gccatatagc aacacatccc
     7441 gagcatgata aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac
     7501 acttaatcga ccaatgccca gcgatgagat aactatcgag acacccgcgg cattagtctg
     7561 ccaaagtgtc tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac
     7621 caggtaatta aaatacaacc caagtgcggt agggtaatag ggatttttca taggttctac
     7681 ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca gtatccattc attattgttg
     7741 taacaacgac aattcctttc tgtggaaaaa ttagcaaagc cgctccagat cgtgtgcata
     7801 taattcagcg gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac
     7861 tcgttgaaat gcggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt
     7921 tattttctct atgatggcat gaagatgttt ttttctttgc tgacgcatag ccaacagttg
     7981 ctcgactaca cgatcaggaa tggcacactt gccattttcc cattgacgcc agctttcact
     8041 gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg
     8101 gagtgcctgg agttcataag cgttcatcgt gtaatggctg catgaaataa acaataaaat
     8161 ataaataaca ttatcagcct gctgacggct tgaagagagt cacagtatct tgtgcaacat
     8221 tattggaaag agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa aagcccatcg
     8281 tgggagatgg gcaaagacta cacacagcaa ttcgttgttt cactcagggg atttccatgc
     8341 ttataaatca atatgttgat ttataaccgt gagctaatag taggcatacg gactttttgc
     8401 gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca ggtactacca
     8461 gctgaatggc ggtaagcagg aaaatatggg gaagagcaag caaatatcaa ttagcggtgg
     8521 ttaacaatca ggcggcagag ccgcctgatg agaaattact tattcggttt ctcgatttcg
     8581 ccaagttctt cgagaatttc ttccggttgg tccgtcggcg gcgggacttc ttccacccac
     8641 gcggcaaaca gacgccagga aacggctaac agaaccggac caataaacag accgatcatc
     8701 ccgaaagcaa tcaaaccacc aataacgcca gagaggatca ggatcagcgg taaatcggca
     8761 cccatgcgaa ttaacattgg gcggatgacg ttatccagcg tgccaaccac accgctccac
     8821 actaacaata ccgttcccca ggtggtatcg ccggtccagt agagccagat aatcgccgga
     8881 atcagtaccg gcaacgggcc aagctggaca aggcaggaga ggatcattaa caccgttagc
     8941 aacgttgcat aaggtacgcc ggatacggcg aggccgatac cgccaagcac tgcctgtact
     9001 aacgccgtca ccaccacacc cagcgccacc gcgcggatag cctgtgccgc cagcagtacg
     9061 gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc ttgtgccacc
     9121 tgttcaccgc gccagtacag cagggcactg aagagaagca tcagcgcaca atgcaccata
     9181 aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg tggtggtgcc aatataaggg
     9241 cggactttcg ccatgatcgc cgtccccccc atatccagca agttgtgcca gcctgcatac
     9301 agcttcgcgc caatcaccgg aatggtatta agccacgcca gatcgggtaa cgtcatgtcg
     9361 ccgctggaaa tggctttaat tagcgggccg ctgccgtcga cgatactgtt aaccagcaaa
     9421 gcaataggga tgataaacac catcactaat aacagcgtca tcaccagaac ggcgagagag
     9481 cggcggccaa acatgatctt ttgcaaacgt aacaatactg gccaggtggc gataaccacc
     9541 gtaccggccc atgcaaagcc gagaataaag ggttgaacaa tccacagaca tgccacaatc
     9601 atgatggcta aaaacagcac cgaaagcaga atttgtgcga catccctggg ctgacgaaca
     9661 tttaccataa ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg
     9721 cgaaactcac ctgcataatg atgagcaatt tcagcggttt ttaacaggcc gattctgccc
     9781 ttaattctga tgggcgtgtt agacaaaaat gtggtacaac aactcaggca acacgcaaac
     9841 gatttactcg tcgtatttca agttgcatgt gctgcggcta cgttcgttca ccccagtcac
     9901 ttactattgt aagctcctgg agttcactcg cttgccgcct tcctgcaact cgaaatccat
     9961 agagtaaata aacatcacct ttcagtgcgc tattgcagcc gcctgaaaga atagtgtatc
    10021 gacctacatc acagaccgca ggaaagggtc aatataatga ttccacagat ttcccaggca
    10081 cccggcgtcg ttcaactggt gcttaatttt ttgcaagagc tggagcaaca aggttttacc
    10141 ggcgatacgg cgacaagtta tgccgatcgt ctgacaatgt cgaccgacaa cagtatttac
    10201 caacttctcc ccgatgcggt ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc
    10261 cgtcttgccg cgcaggaacg ctattcatcg ctgatcttta ccccccgcgg cggcggcacc
    10321 ggcactaacg gtcaggcgct caaccagggg attattgttg atatgtcccg ccatatgaac
    10381 cgcatcatcg aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa
    10441 gatcaactca atcagtacct gaaaccgttc ggctactttt ttgcgccgga actttcgacc
    10501 agcaaccggg caacgctcgg tgggatgatc aataccgatg catccggtca gggatcgctg
    10561 gtctatggca aaacgtcgga tcacgtactt ggcgtacgcg cggtgttgtt ggggggcgat
    10621 attctcgata cgcaaccttt acccgtcgaa ctggcggaaa cgctgggtaa atccaatacc
    10681 acaatcgggc gaatttataa cacggtttat caacgttgcc gtcagcaacg ccagttaatc
    10741 atcgacaact tccccaaact taaccgcttt cttaccggtt acgatctgcg tcatgtcttt
    10801 aacgatgaga tgaccgagtt cgacctgacg cgcattctga cgggttcaga agggacgctg
    10861 gcctttatta ccgaagcgcg gctggatatt acgcccttgc ctaaagtgcg ccgtctggtg
    10921 aacgtcaaat atgactcttt tgactccgcg ctgcgtaacg cgccgtttat ggttgaggcg
    10981 cgggcgcttt cggtagagac ggtggactca aaagtgctga atctggcgcg ggaagatatt
    11041 gtctggcatt ccgtcagcga gttgattacc gatgtgcctg acaaagagat gctcgggctg
    11101 aacattgtgg agtttgctgg tgatgatgag gcgctgattg acgagcgagt taatgctctc
    11161 tgtgtgcggc ttgatgagct gattgccagc caacaggcag gtgtgattgg ctggcaggtg
    11221 tgccgcgagc tggcgggtgt tgaacgtatc tatgcgatgc gtaaaaaagc cgttggtctg
    11281 cttggcaatg ccaaaggtgc cgctaagcca attccgtttg ctgaggatac ctgcgtaccg
    11341 ccggaacatc tggcggatta tattgctgaa tttcgcgcgc tgctcgacag ccacggctta
    11401 agctacggta tgttcggtca cgtcgacgca ggtgttttgc acgtccgtcc agcgctggat
    11461 atgtgcgatc ctcaacaaga gattttgatg aagcaaatct ctgatgacgt ggtggcgctg
    11521 actgcgaaat acggtggttt gttgtggggc gagcacggca aaggttttcg cgctgaatac
    11581 agcccggcgt ttttcggtga ggaacttttt gcagaactgc gcaaagtgaa agcggcattt
    11641 gacccgcata accgactcaa cccagggaag atttgcccgc cagaaggtct cgatgcgccg
    11701 atgatgaaag tggacgcggt gaagcgcggt acattcgatc ggcagatccc cattgcggta
    11761 cgccagcagt ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt caactttgat
    11821 gcccgtagtc cgatgtgtcc gtcgatgaag atcacccaga accggattca ttcaccgaaa
    11881 gggcgcgcaa cgctggtgcg tgaatggctg cgtttgttgg cggatcgcgg cgttgatcca
    11941 ctcaaactgg aacaagaact gcctgaatcc ggcgtcagtt tgcggacgtt aattgcccgc
    12001 acgcgcaata gctggcatgc gaataaaggc gaatatgact tctcacacga agtcaaagag
    12061 gcgatgtcgg gctgtctggc ctgtaaagcg tgttcgaccc agtgccccat caaaattgat
    12121 gtgccggagt ttcgctctcg ttttctgcag ctctatcaca cccgttattt acgcccgctg
    12181 cgcgaccacc tcgtcgctac ggtcgagagc tacgcgccgc tgatggcacg cgcgccgaaa
    12241 acctttaact tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa acatatcggc
    12301 atggttgatt tgccactgct gtcggtcccc tcgctacaac aacaaatggt ggggcatcgc
    12361 tcggcaaaca tgacgctgga acagcttgaa gctctcaatg cagagcagaa agcgcgcacg
    12421 gtgttggtgg tgcaggaccc ctttaccagc tattacgatg cgcaagtggt ggcggatttt
    12481 gtccgtctgg ttgaaaaatt aggtttccag ccggtgttac tgccattttc gccaaatggc
    12541 aaagcccagc atattaaagg ttttcttaat cgttttgcga agacggcgaa aaagacggcg
    12601 gatttcctca accgtatggc gaagctgggg atgccaatgg tgggcgtcga tccggcgctg
    12661 gtactttgtt atcgcgatga atataaactg gcgctgggcg aggaacgtgg cgagtttaac
    12721 gtcttactgg cgaatgaatg gctggcaagc gcacttgagt cacagccagt ggctacagtc
    12781 agcggtgaat catggtattt ctttggtcac tgtaccgagg ttaccgcctt gccgggcgcg
    12841 ccagcacaat gggccgcgat atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg
    12901 ggttgctgcg gcatggcagg gacttacgga catgaagaga aaaaccataa aaattcgctc
    12961 gggatctatg agttatcctg gcatcaggct atgcagcgac tgccgcgtaa ccgctgtctg
    13021 gcgaccggat attcctgccg tagccaggtc aaacgggttg aaggcacggg ggtacgccat
    13081 cctgtgcagg ctttactgga gattattaaa tgatatggaa acgaaaaatc accctggaag
    13141 cactgaatgc tatgggtgaa ggaaacatgg tggggttgct ggatattcgc tttgaacata
    13201 ttggtgatga cacccttgaa gcgacaatgc cagtagactc gcggacaaag cagcctttcg
    13261 ggttgctgca tggaggcgca tccgtggtgc tggccgaaag tatcggttcc gttgccggtt
    13321 atttatgtac tgaaggtgag caaaaagtgg ttggcctgga aatcaatgct aaccacgtcc
    13381 gctcggcacg agaagggcgg gtgcgcggcg tatgcaaacc gttgcatctc ggttcgcgtc
    13441 accaggtctg gcagattgaa atcttcgatg agaaagggcg tttgtgctgt tcgtcacgat
    13501 tgacgaccgc cattttgtga cgttgtcacc agaaaagtgt gacgcatatt gcttttggtc
    13561 attatctgca cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc gaggatgcta
    13621 tgtctactca acttgatccc acccagctgg ccattgaatt tttacgccgt gaccagagca
    13681 atctttcacc tgcccaatat ctcaaacgtc tgaaacaact agaacttgaa tttgctgacc
    13741 tgcttactct ctcttctgct gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg
    13801 tgcattgatc ctgacttaat gccgcaagtt cgccaaatcg acctggatta acctccgtcc
    13861 aggttcgatg accttcttaa tactgcttat ctacccgcct gatattgttc aaaaataata
    13921 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
    13981 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
    14041 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
    14101 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
    14161 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
    14221 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
    14281 gtttgctggg ttgaactgat aatcattatc actaacatgc tgttatacgc tgaaagcgat
    14341 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
    14401 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
    14461 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
    14521 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
    14581 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
    14641 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
    14701 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
    14761 gatgtcaaaa catggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
    14821 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
    14881 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
    14941 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagc
    15001 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
    15061 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
    15121 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
    15181 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
    15241 ggtgaggcga tccacgatca cccggaactg gtgcgtaaat atctcggcac cgtggtgccg
    15301 gggaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
    15361 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
    15421 gaaaaaactg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
    15481 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcagtggtg
    15541 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
    15601 ggcgataaca acaccggcgg tattctcaac ttcgtcacca agcgtgcttt gtgcgaaggc
    15661 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
    15721 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
    15781 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
    15841 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
    15901 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
    15961 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
    16021 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
    16081 caacgcggga tcagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
    16141 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
    16201 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
    16261 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
    16321 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
    16381 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
    16441 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
    16501 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
    16561 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
    16621 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
    16681 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
    16741 gagtcggact ccgggctgga tattgacgca ttaaaagtgg tcgccgatgg cgtgaactcg
    16801 ctgcgtgatg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
    16861 atcaagcctg attacgttca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
    16921 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
    16981 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
    17041 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
    17101 atatacgccg ctggaagggc tgaccaatag ccagtttgtc agcattgcgg gagagatatc
    17161 cccacagcag cgagatgcct tagcgttaac gttagacgct gtgcggctgg tatttgtcga
    17221 tggacgttac gtgtcagcac tgagcgatgc gactgaaggc agcgggtatg aagtgagcat
    17281 taacgacgac cgtcaggggg tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
    17341 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
    17401 gccattgctg ttaatgcata ttacccaggg cgtggcaggt gaagaggtca acactgccca
    17461 ttaccgacat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
    17521 cagccttaat gatgctcgtc acttcaccgg cgcacggttc actatcaatg tcgcagcgaa
    17581 cgcccaattg cagcatatca agctggcgtt tgaaaacccg gtcagtcacc actttgccca
    17641 taacgatttg ttgctggctg acgatgccac cgcatttagc catagtttcc tgctgggtgg
    17701 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
    17761 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
    17821 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
    17881 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
    17941 gaccaacaat aatctgctga tgggcaaact ggcagaagtg gatacgaaac cgcagctgga
    18001 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
    18061 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
    18121 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
    18181 gctggcccga atcggtcaac gactgccagg aggtgcaaga tgactttttc cgtcgacaaa
    18241 gtgcgggccg actttccggt gctttcgcgt gaggtaaacg gtttgccgct ggcttatctc
    18301 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg actccgaagc cgagttttat
    18361 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
    18421 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    18481 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    18541 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    18601 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat cccgctcaat
    18661 cccgacggta cgctgcaact ggagacgcta cctaatctgt tcgatgagaa aactcgcctg
    18721 ctggcaatta ctcatgtctc caacgtgctt ggcacagaaa atcccctggc ggaaatgatc
    18781 acgcttgcgc atcagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    18841 catccggtgg acgttcaggc gctggattgc gatttttacg tattctccgg gcataaactg
    18901 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    18961 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    19021 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat cattggtctt
    19081 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    19141 aatctgatgc actacgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    19201 ccgcggaaca ggcttggcgt tattgctttt aatcttggta aacaccacgc ctatgatgtt
    19261 ggcagttttc tcgataacta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    19321 ttaatggcct attacaacgt ccctgctatg tgtcgggcgt cgctggccat gtataacacc
    19381 catgaagaag tggatcgtct ggtgaccgga ctgcaacgta ttcaccgtct gctgggataa
    19441 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    19501 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgccagaa
    19561 ttacgcgccg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    19621 attgtcatgc gccagaatgc gcagggaatt attgaattgc agggcgacag cgatgcggcg
    19681 attgtgaaag ggcttattgc ggtcgtcttt attctctacg atcagatgac gccgcaggat
    19741 attgtcaatt tcgatgtgcg tccgtggttt gaaaaaatgg cgctcaccca acatctcacc
    19801 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    19861 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    19921 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    19981 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    20041 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    20101 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    20161 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    20221 caataacact atcgccccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    20281 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    20341 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    20401 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    20461 gcctacggca ggcattcgtc aacgttcgct ggagcgtggc attaaattac cgccagtcgt
    20521 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    20581 cgaatacctc attcatggta ccagtgcgcc ggacagcgtc ggtttgcgcg tcagttcagg
    20641 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    20701 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    20761 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    20821 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    20881 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    20941 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    21001 tatgttacgc gcgacgcagt agcggtaaac ggcagacaaa aaaaatggcg cacaatgtgc
    21061 gccatttttc acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac
    21121 gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca
    21181 cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat
    21241 cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca
    21301 gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc
    21361 gttacaagta ttacacaaag ttttttatgt tgagaatatt tttttgatgg gaaggcactt
    21421 atttttgatc gttcgctcaa agaagcatcg aacgctgggt tttagataaa aaaattgaga
    21481 gaaaacagga tgcttccat
//
LOCUS       DAZFFI010000077        21436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFFI010000077 DAZFFI010000000
VERSION     DAZFFI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.077"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            89..634
                     /locus_tag="ACHJ12_003355"
     CDS             89..634
                     /locus_tag="ACHJ12_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4991093.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            609..2534
                     /locus_tag="ACHJ12_003356"
     CDS             609..2534
                     /locus_tag="ACHJ12_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4991094.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEEGAAT
                     NKKTLADYARALSGEDE"
     gene            2531..2737
                     /locus_tag="ACHJ12_003357"
     CDS             2531..2737
                     /locus_tag="ACHJ12_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4991095.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2734..4335
                     /locus_tag="ACHJ12_003358"
     CDS             2734..4335
                     /locus_tag="ACHJ12_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4991096.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQWERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4316..5635
                     /locus_tag="ACHJ12_003359"
     CDS             4316..5635
                     /locus_tag="ACHJ12_003359"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4991097.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMPKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5645..5977
                     /locus_tag="ACHJ12_003360"
     CDS             5645..5977
                     /locus_tag="ACHJ12_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4991098.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6033..7058
                     /locus_tag="ACHJ12_003361"
     CDS             6033..7058
                     /locus_tag="ACHJ12_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4991099.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7100..7498
                     /locus_tag="ACHJ12_003362"
     CDS             7100..7498
                     /locus_tag="ACHJ12_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4991100.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7510..7863
                     /locus_tag="ACHJ12_003363"
     CDS             7510..7863
                     /locus_tag="ACHJ12_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4991101.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7875..8453
                     /locus_tag="ACHJ12_003364"
     CDS             7875..8453
                     /locus_tag="ACHJ12_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4991102.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8450..8845
                     /locus_tag="ACHJ12_003365"
     CDS             8450..8845
                     /locus_tag="ACHJ12_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4991103.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8853..9593
                     /locus_tag="ACHJ12_003366"
     CDS             8853..9593
                     /locus_tag="ACHJ12_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4991104.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            9609..10031
                     /locus_tag="ACHJ12_003367"
     CDS             9609..10031
                     /locus_tag="ACHJ12_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4991105.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10013..10447
                     /locus_tag="ACHJ12_003368"
     CDS             10013..10447
                     /locus_tag="ACHJ12_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4991106.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPVSPDVADMTEDDVMLMTVSEGIAGGIRYG"
     gene            10440..13001
                     /locus_tag="ACHJ12_003369"
     CDS             10440..13001
                     /locus_tag="ACHJ12_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4991107.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            12998..13327
                     /locus_tag="ACHJ12_003370"
     CDS             12998..13327
                     /locus_tag="ACHJ12_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4991108.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13327..14025
                     /locus_tag="ACHJ12_003371"
     CDS             13327..14025
                     /locus_tag="ACHJ12_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4991109.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNAKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWIYRGDECGYNGPAVADEYDQPTSDISKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14031..14774
                     /locus_tag="ACHJ12_003372"
     CDS             14031..14774
                     /locus_tag="ACHJ12_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4991110.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPNFHRGDDWWRHGQNLYLDNLEDT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            14771..15343
                     /locus_tag="ACHJ12_003373"
     CDS             14771..15343
                     /locus_tag="ACHJ12_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4991111.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRISGRDVSTSGLTAQ
                     LHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TSILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15404..18802
                     /locus_tag="ACHJ12_003374"
     CDS             15404..18802
                     /locus_tag="ACHJ12_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4991112.1"
                     /translation="MGKGGSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TVEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVESVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            18869..19468
                     /locus_tag="ACHJ12_003375"
     CDS             18869..19468
                     /locus_tag="ACHJ12_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4991113.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            19533..>21436
                     /locus_tag="ACHJ12_003376"
     CDS             19533..>21436
                     /locus_tag="ACHJ12_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4991114.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTAITKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSTTNSTSETLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMP"
BASE COUNT         4986 a         5505 c         6692 g         4253 t
ORIGIN      
        1 tctgaaaaga aaggaaacga caggtgctga aagcgagctt tttggcctct gtcgtttcct
       61 ttctctgttt ttgtccgtgg aatgaacaat ggaagtcaac aaaaagcagc tggctgacat
      121 tttcggtgcg agtatccgta ccattcagaa ctggcaggaa cagggaatgc ccgttctgcg
      181 aggcggtggc aagggtaatg aggtgcttta tgactctgcc gccgtcataa aatggtatgc
      241 cgaaagggat gctgaaattg agaacgaaaa actgcgccgg gaagttgaag aactgcggca
      301 ggccagcgag acagatctcc agccagggac tattgagtac gaacgccatc gacttacgcg
      361 tgcgcaggcc gacgcacagg agctgaaaaa tgccagagac tccgctgaag tggtggaaac
      421 cgcattctgt actttcgtgc tgtcgcggat cgcaggtgaa attgccagta ttctcgacgg
      481 gatccccctg tcggtgcagc ggcgttttcc ggaactggaa aaccgacatg ttgatttcct
      541 gaaacgggat atcatcaaag ccatgaacaa agcagccgcg ctggatgaac tgataccggg
      601 gttgctgagt gaatatatcg aacagtcagg ttaacaggct gcggcatttt gtccgcgccg
      661 ggcttcgctc actgttcagg ccggagccac agaccgccgt tgaatgggcg gatgccagtt
      721 actatctccc aaaagaatcc gcataccagg aagggcgctg ggaaacactg ccctttcagc
      781 gggccatcat gaatgcgatg ggcagcgact acatccgtga ggtgaatgtg gtgaagtctg
      841 cccgtgtcgg ttattccaaa atgctgctgg gtgtttatgc ctactttata gagcataagc
      901 agcgcaacac ccttatctgg ttgccgacgg atggtgatgc cgagaacttt atgaaaaccc
      961 acgttgagcc gaccatccgc gatattccgt cgctgctggc gctggctccg tggtatggca
     1021 aaaagcaccg ggataacacg ctcactatga agcgtttttc caatggtcgt ggcttctggt
     1081 gcctgggcgg taaagcggca aaaaactacc gtgaaaagtc ggtggatgtg gcgggttatg
     1141 atgaacttgc tgcctttgat gaggatattg aacaggaagg ctctccgacg ttccttggcg
     1201 acaaacgtat tgaaggctcg gtctggccaa agtccatccg tggctccacg cccaaagtga
     1261 gaggcacctg ccagattgag cgtgcagcca gtgaatcccc gcattttatg cgttttcatg
     1321 ttgcctgccc gcactgcggg gaggagcagt atcttaaatt tggcgacaaa gagacgccgt
     1381 ttggcctcaa atggacgccg gatgacccct ccagcgtgtt ttatctctgc gagcataatg
     1441 cctgcgtcat ccgccagcag gagctggact ttactgatgc ccgttatatc tgcgaaaaga
     1501 ccgggatctg gacccgtgat ggcattctct ggttttcgtc atccggtgaa gagattgagc
     1561 cgccggacag tgtgaccttt cacatctgga cggcgtacag cccgttcacc acctgggtgc
     1621 agattgtcaa agactggatg aagacgaaag gggatacggg aaaacgtaaa accttcgtga
     1681 acaccacgct cggtgagaca tgggaagcga aaattggcga acgtccggat gctgaggtga
     1741 tggcggagcg gaaagagcat tattcagcgc ccgttcctga ccgtgtggct tacctgaccg
     1801 ccggtatcga ctcccagctg gaccgctacg aaatgcgcgt atggggatgg gggccgggtg
     1861 aggaaagctg gctgattgac cggcagatta ttatgggccg ccacgacgat gaacagacgc
     1921 tgctgcgtgt ggatgaggcc atcaataaaa cctatacccg ccggaatggt gcagaaatgt
     1981 cggtatcccg tatctgctgg gatactggcg ggattgaccc gaccattgtg tatgaacgct
     2041 cgaaaaagca tgggctgttc cgggtgatcc ccattaaagg ggcatccgtc tacggaaagc
     2101 ctgtggccag catgccacgt aagcgaaaca aaaacggggt ttaccttacc gaaattggta
     2161 cggataccgc gaaagagcag atttataacc gcttcacact gacgccggaa ggggatgaac
     2221 cgcttcccgg tgccgttcac ttcccgaata acccggatat ttttgatctg accgaagcgc
     2281 agcagctgac tgctgaagag caggtcgaaa aatgggtgga tggcaggaaa aaaatactgt
     2341 gggacagcaa aaagcgacgc aatgaggcgc tcgactgctt cgtttatgcg ctggcggcgc
     2401 tgcgcatcag tatttcccgc tggcagctgg atctcagtgc actgctggcg agcctgcagg
     2461 aagaggaggg tgcagcaacc aacaagaaaa cactggcaga ttacgcccgt gccttatccg
     2521 gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac tgcatgacct
     2581 gatgacaggt aaacgggtgg caacggtaca gaaagacgga cggcgagtgg agtttacggc
     2641 cacttccgtg tctgacctga aaaaatacat tgcggagctg gaagtgcaga ccggcatgac
     2701 acagcgacgc aggggacctg caggatttta tgtatgaaaa cgtccaccat tcccaccctt
     2761 ctggggccgg acggcatgac atcgctgcgt gaatatgccg gttatcacgg cggtggcagc
     2821 ggatttggtg ggcagttgcg ggcgtggaac ccaccgagtg aaagtgtgga tgcagccctg
     2881 ctgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa taacggctat
     2941 gccgccaacg ccatccagtt gcatcaggat catatcgtcg ggtctttttt ccggctcagt
     3001 catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc
     3061 gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag
     3121 cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt
     3181 gaactgttcg ttcaggccac ctgggatacc agtccgtcgc ggcttttccg gacacagttc
     3241 cggatggtaa gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc
     3301 cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac
     3361 ggctatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg
     3421 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagacccg cggtgcaaat
     3481 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagctg
     3541 cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct ggatacgcag
     3601 tcagcgatgg attttattct gggcgcgaac agtcaggagc agtgggaaag gctgaccggc
     3661 tggattggtg aaattgccgc gtattacgcc gcagcaccgg tccgtctggg aggcgcaaaa
     3721 gtgccgcacc tgatgccggg ggactcactg aacctgcaga cggctcagga cacggataac
     3781 ggctactccg tgtttgagca gtcactgttg cggtatatcg ctgccgggct gggtgtctcg
     3841 tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg ggccagtgcg
     3901 aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg tcaggcgagc
     3961 cagatgtttc tgtgctggct ggaagaggcc atcgctcgcc gcgtggtgac gttaccttca
     4021 aaagcacgct tcagttttca ggaagcccga agtgcctggg ggaactgcga ctggataggc
     4081 tccggtcgta tggccatcga tggtctgaaa gaagtacagg aagcggtgat gctgatagaa
     4141 gccggactga gtacctacga gaaagagtgc gcaaaacgcg gtgacgacta tcaggaaatt
     4201 tttgcccagc aggtccgtga aacgatggag cgccgtgcag ccggtcttaa accgcccgcc
     4261 tgggcggctg cggcatttga atccgggctg cgacaatcaa cagaggagga gaagagtgac
     4321 agcagagctg cgtaatctcc cgcatattgc cagtatggcc tttaatgagc cgctgatgct
     4381 tgaacccgcc tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg ggatcagccg
     4441 cctgacggat gcggtatccg gcgacagcct gactgccgga gaggcacccg cggcgctggc
     4501 gttatccgtt gatgatgacg gaccacgaca ggcccgcagt tatcaggtca tgaacggcat
     4561 cgccgtgctg ccggtgtccg gcacgctggt cagtcggacg cgagcgctgc agccgtattc
     4621 ggggatgacc ggttacaacg gcattatcgc ccgtctgcaa caggctgcca gcgacccgat
     4681 ggtggacggc attctgctcg atatggacac gcccggcggg atggtggcgg gggcatttga
     4741 ctgcgctgac atcatcgccc gtgtgcgtga cataaaaccg gtatgggcgc ttgccaacga
     4801 catgaactgc agtgcaggtc agttgcttgc cagtgccgcc tcccggcgtc tggtcacgca
     4861 gaccgcccgg acaggctcca tcggcgtcat gatggctcac agtaattacg gtgctgcgct
     4921 ggagaaacag ggtgtggaaa tcacgctgat ttacagcggc agccataagg tggatggcaa
     4981 cccctacagc catcttccgg atgacgtccg ggagacactg cagtcccgga tggacgcaac
     5041 ccgccagatg tttgcgcaga aggtgtcggc atataccggc ctgtccgtgc aggctgtgct
     5101 ggataccgag gctgcagtgt acagcggtca ggaggccatt gatgccggac tggctgatga
     5161 acttgttaac agcaccgatg cgatcaccgt catgcgtgat gcactggatg cacgtaaatc
     5221 ccgtctctca ggagggcgaa tgcccaaaga gactcaatca acaactgttt cagccactgc
     5281 ttcgcaggct gacgttactg acgtggtgcc agcgacggag ggcgagaacg ccagcgcggc
     5341 gcagccggac gtgaacgcgc agatcaccgc agcggttgcg gcagaaaaca gccgcattat
     5401 ggggatcctc aactgtgagg aggctcacgg acgcgaagaa caggcacgcg tgctggcaga
     5461 aacccccggt atgaccgtga aaacggcccg ccgcattctg gccgcagcac cacagagtgc
     5521 acaggcgcgc agtgacactg cgctggatcg tctgatgcag ggggcaccgg caccgctggc
     5581 tgcaggtaac ccggcatctg atgccgttaa cgatttgctg aacacaccag tgtaagggat
     5641 gtttatgacg agcaaagaaa cctttaccca ttaccagccg cagggcaaca gtgacccggc
     5701 tcataccgca accgcgcccg gcggattgag tgcgaaagcg cctgcaatga ccccgctgat
     5761 gctggacacc tccagccgta agctggttgc gtgggatggc accaccgacg gtgctgccgt
     5821 tggcattctt gcggttgctg ctgaccagac cagcaccacg ctgacgttct acaagtccgg
     5881 cacgttccgt tatgaggatg tgctctggcc ggaggctgcc agcgacgaga cgaaaaaacg
     5941 gaccgcgttt gccggaacgg caatcagcat cgtttaactt tacccttcat cactaaaggc
     6001 cgcctgtgcg gcttttttta cgggattttt ttatgtcgat gtacacaacc gcccaactgc
     6061 tggcggcaaa tgagcagaaa tttaagtttg atccgctgtt tctgcgtctc tttttccgtg
     6121 agagctatcc cttcaccacg gagaaagtct atctctcaca aattccggga ctggtaaaca
     6181 tggcgctgta cgtttcgccg attgtttccg gtgaggttat ccgttcccgt ggcggctcca
     6241 cctctgaatt tacgccggga tatgtcaagc cgaagcatga agtgaatccg cagatgaccc
     6301 tgcgtcgcct gccggatgaa gatccgcaga atctggcgga cccggcttac cgccgccgtc
     6361 gcatcatcat gcagaacatg cgtgacgaag agctggccat tgctcaggtc gaagagatgc
     6421 aggcagtttc tgccgtgctt aagggcaaat acaccatgac cggtgaagcc ttcgatccgg
     6481 ttgaggtgga tatgggccgc agtgaggaga ataacatcac gcagtccggc ggcacggagt
     6541 ggagcaagcg tgacaagtcc acgtatgacc cgaccgacga tatcgaagcc tacgcgctga
     6601 acgccagcgg cgtggtgaat atcatcgtgt tcgatccgaa aggctgggcg ctgttccgtt
     6661 ccttcaaagc cgtcagggag aagctggata cccgtcgtgg ctctaattcc gagctggaga
     6721 cagcggtgaa agacctgggc aaagcggtgt cctataaggg gatgtatggc gatgtggcca
     6781 tcgtcgtgta ttccggacag tacgtggaaa acggcgtcaa aaagaacttc ctgccggaca
     6841 acacgatggt gctggggaac actcaggcac gcggtctgcg cacctatggc tgcattcagg
     6901 atgcggacgc acagcgcgaa ggcattaacg cctctgcccg ttacccgaaa aactgggtga
     6961 ccaccggcga tccggcgcgt gagttcacca tgattcagtc agcaccgctg atgctgctgg
     7021 ctgaccctga tgagttcgtg tccgtacaac tggcgtaatc atggcccttc ggggccattg
     7081 tttctctgtg gaggattcca tgacgaaaga tgaactgatt gcccgtctcc gctcgctggg
     7141 tgaacaactg aaccgtgatg tcagcctgac ggggacgaaa gaagaactgg cgctccgtgt
     7201 ggcagagctg gaagaggagc ttgatgacac ggatgaaact gccggtcagg acacccctct
     7261 cagccgggaa aatgtgctga ccggacatga aaatgaggtg ggatcagcgc agccggatac
     7321 cgtgattctg gatacgtctg aactggtcac ggtcgtggca ctggtgaagc tgcatactga
     7381 tgcacttcac gccacgcggg atgaacctgt ggcatttgtg ctgccgggaa cggcgtttcg
     7441 tgtctctgcc ggtgtggcag ccgaaatgac agaacgtggc ctggccagaa tgcaataacg
     7501 ggaggcgctg tggctgattt cgataacctg ttcgatgctg ccattgcccg cgccgatgaa
     7561 acgatacgcg ggtacatggg aacgtcagcc accatgacat ccggtgagca gtccggcgca
     7621 gtaatacgtg gtgtttttga tgaccctgaa aatatcagct atgccggaca gggcgtgcgc
     7681 gttgaaggct ccagcccgtc cctgtttgtc cggactgatg atgtgcggca gctgcggcgc
     7741 ggcgacacgc tgaccatcgg tgaggaaaac ttctggatag accggatttc gccggatgat
     7801 ggcggaagct gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa ccgtcgccgc
     7861 tgaaaggggg atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac ctcagccgta
     7921 tcagcagaac ggcggtgccc ggtgccgccg caatggctat taaccgcgtt gcttcatccg
     7981 cgatatcgca gtcggcgtca caggttgccc gtgagacaaa ggtacgccgg aaactggtaa
     8041 aggaaagggc caggctgaaa agggccacgg tcaaaaatcc gcaggccaga atcaaggtta
     8101 accgggggga tttgcccgta ataaagctgg gtaacgcgcg gattgtcctg tcccgacgca
     8161 ggcgtcgtaa aaaggggcag cgttcatccc tgaaaggtgg cggcagcgtg cttgtggtgg
     8221 gtaaccgtcg tattcccggc gcgtttattc agcaactgaa aaatggccgg tggcatgtca
     8281 tgcagcgtgt ggccgggaaa aaccgttacc ccattgatgt agtgaaaatc ccgatggcgg
     8341 tgccgctgac cacggcgttt aaacaaaata ttgagcggat acggcgtgaa cgtcttccga
     8401 aagagctggg ctatgcgctg cagcatcaac tgaggatggt aataaagcga tgaaacatac
     8461 tgaactccgt gcagccgtac tggatgcact ggagaagcat gacaccgggg cgacgttttt
     8521 tgatggtcgc cccgctgttt ttgatgaggc ggattttccg gcagttgccg tttatctcac
     8581 cggcgctgaa tacacgggcg aagagctgga cagcgatacc tggcaggcgg agctgcatat
     8641 cgaagttttc ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga tggagtcccg
     8701 gatttatccg gtgatgagcg atatcccggc actgtcagat ttgatcacca gtatggtggc
     8761 cagtggctat gactaccggc gcgacgatga tgcgggcctg tggagttcag ccgatctgac
     8821 ttatgtcatt acctatgaaa tgtgaggacg atatgccaac accaaatcct ctggcaccgg
     8881 tgaaaggggc cgggaccaca ctgtgggttt ataacgggaa cggcgaccca tatgcaaacc
     8941 cgctttcaga caatgactgg tcgcgtctgg caaaggttaa agacctgacg cccggagaac
     9001 tgaccgctga gtcctatgac gacagttatc tcgatgatga agatgcggac tgggccgcga
     9061 ccggacaggg gcagaaatcc gctggagata ccagcttcac gctggcgtgg atgcccggag
     9121 agcaggggca gcaggcgctg ctggcgtggt ttaatgaagg tgatacccgt gcctataaaa
     9181 tccgcttccc gaacggcacg gtcgatgtgt tccgcggctg ggtcagcagt atcggtaagg
     9241 cggtgacggc gaaggaagtg atcacccgca cggtgaaagt caccaacgtg ggacgtccgt
     9301 cgatggcaga agatcgcagc acggtaacag cggcaaccgg catgaccgtg acgcctgcca
     9361 gcacctcggt ggtgaaaggg cagagcacga cgctgaccgt ggcattccag ccggaaggcg
     9421 caaccgacaa gagcttccgt gcggtgtctg cggataaaac aaaagccacc gtgtcggtca
     9481 gtggtatgac catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt ccggtcgtat
     9541 ccggtaatgg tgagtttgct gcggttgcag aaatcaacgt caccgccagt taatccggag
     9601 aatcagcgat gttcctgaaa accgaatcat ttgaacataa cggcgtgacc gtcacgcttt
     9661 ctgaactgtc agccctgcag cgtattgagc atctcgccct gatgaaacgg caggcagaac
     9721 aggcggagtc agacagcaac cgaaagttta ctgtggaaga cgccatcaga accggcgctt
     9781 ttgtggtggc gatgtccctg tggcataacc atccgaagaa gacgcagatg ccgtccatga
     9841 atgaagccgt taaacagatt gagcaggaag tgcttaccac ctggcccgcg gaggcaattt
     9901 ctcatgctga aaacgtggtg taccggctgt ccggtatgta tgagtttgtg gtgaatgatg
     9961 cccctgaaca ggcagaggac gccgggcctg cagagcctgt ttctgcggga aagtgttcga
    10021 cggtgagctg agttttgccc tgaaactggc gcgcgagatg gggcgacccg actggcgtgc
    10081 catgcttgcc gggatgtcat ccacggagta tgccgactgg caccgctttt acagtaccca
    10141 ttattttcat gatgttctgc tggatatgca cttttccggg ctgacgtaca ccgtgctcag
    10201 cctgtttttc agcgatccgg atatgcatcc gctggatttc agtctgctga accggcgtga
    10261 ggctgacgaa gagcctgaag atgatgtgct gatgcagaaa gcggcagggc ttgccggagg
    10321 cgttcgtttt ggcccggacg ggaatgaagt tatccccgtt tccccggatg tggcggacat
    10381 gacggaggat gacgtaatgc tgatgacagt atcagaaggg atcgcaggag gaatccggta
    10441 tggctgaacc ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc agatttgacg
    10501 agcagatggc cagagtcagg cgtcattttt ccggtacgga aagtgatgcg aaaaaaacag
    10561 cggcagtcgt tgaacagtcg ctgagccgac aggcactggc tgcacagaaa gcggggattt
    10621 ccgtcgggca gtataaagct gccatgcgta tgctgcctgc gcagttcacc gacgtggcca
    10681 cgcagcttgc aggcgggcaa agtccgtggc tgatcctgct gcaacagggt ggtcaggtga
    10741 aggactcctt cggcgggatg atccccatgt tcagggggct tgccggtgcg atcaccctgc
    10801 cgatggtcgg ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat gcctggtatc
    10861 agggcaactc aaccctgtcc gatttcaaca aaacgctggt cctttccggc aatcagtcgg
    10921 gtctgacggc agatcgcatg ctggtcctgt ccagagccgg gcaggcggca gggctgacgt
    10981 ttaaccagac cagcgagtca ctcagcgcac tggttaaggc gggagtaagc ggtgaggctc
    11041 agattgcatc catcagccag agtgtggcgc gtttctcctc tgcatccggc gtggaggtgg
    11101 acaaggtcgc tgaagccttc gggaagctga ccacagaccc gacgtcaggg ctgacagcga
    11161 tggcacgcca gttccataac gtgacggcgg agcagattgc gtatgttgct cagttgcagc
    11221 gttccggcga tgaagccggg gcattgcagg cggcgaacga ggccgcaacg aaagggtttg
    11281 atgaccagac ccgccgcctg aaagagaaca tgggcacgct ggagacctgg gcagacagga
    11341 cagcgcgggc cttcaaatcc atgtgggatg cggtgctgga tattggtcgt cctgataccg
    11401 cgcaggagat gctgattaag gcagaggctg cgtttaagaa agcagacgac atctggaatc
    11461 tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg gatgatcgtg
    11521 aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag actcaacagg
    11581 acaaaaatgc gcagcagcag agcgataccg aagcgtcacg gctgaaatat accgaagagg
    11641 cgcagaaggc ttacgaacgg ctgcagacgc cgctggagaa atataccgcc cgtcaggaag
    11701 aactgaacaa ggcactgaaa gacgggaaaa tcctgcaggc ggattacaac acgctgatgg
    11761 cggcggcgaa aaaggattat gaagcgacgc tgaaaaagcc gaaacagtcc ggcgtgaagg
    11821 tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg cttcaggcag
    11881 aactccggac gctggagaag catgccggag cgaatgagaa aatcagccag cagcgccggg
    11941 atttgtggaa ggcagaaagt cagttcgcgg tactggagga ggcggcacaa cgtcgccagc
    12001 tgtccgcaca ggagaaatcc ctgctggcgc ataaagatga gacgctggag tacaaacgcc
    12061 agctggctgc acttggcgac aaggttacgt atcaggagcg cctgaacgcg ctggcgcagc
    12121 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
    12181 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
    12241 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
    12301 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
    12361 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agccacgcag acctttgatg
    12421 gtattgcaca gaatatggcg gcgatgctga ccggcagtga gcagaactgg cgcagcttca
    12481 cccgttccgt gctgtccatg atgacagaaa ttctgcttaa gcaggcaatg gtggggattg
    12541 tcgggagtat cggcagcgcc attggcgggg ctgttggtgg cggcgcatcc gcgtcaggcg
    12601 gtacagccat tcaggccgct gcggcgaaat tccattttgc aaccggagga tttacgggaa
    12661 ccggcggcaa atatgagcca gcggggattg ttcaccgtgg tgagtttgtc ttcacgaagg
    12721 aggcaaccag ccggattggc gtggggaatc tttaccggct gatgcgcggc tatgccaccg
    12781 gcggttatgt cggtacaccg ggcagcatgg cagacagccg gtcgcaggcg tccgggacgt
    12841 ttgagcagaa taaccatgtg gtgattaaca acgacggcac gaacgggcag ataggtccgg
    12901 ctgctctgaa ggcggtgtat gacatggccc gcaagggtgc ccgtgatgaa attcagacac
    12961 agatgcgtga tggtggcctg ttctccggag gtggacgatg aagaccttcc gctggaaagt
    13021 gaaacccggt atggatgtgg cttcggtccc ttctgtaaga aaggtgcgct ttggtgatgg
    13081 ctattcccag cgagcgcctg ccgggctgaa tgccaacctg aaaacgtaca gcgtgacgct
    13141 ttctgtcccc cgtgaggagg ccacggtact ggagtcgttt ctggaagagc acgggggctg
    13201 gaaatccttt ctgtggacgc cgccttatga gtggcggcag ataaaggtga cctgcgcaaa
    13261 atggtcgtcg cgggtcagta tgctgcgtgt tgagttcagc gcagagtttg aacaggtggt
    13321 gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtcg
    13381 gccagcgtgg tgctctggga aatcgacctg acagaggtcg gtggagaacg ttattttttc
    13441 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcgaca gtatcagccg
    13501 tatcccattc aggggagcgg ttttgaactg aatgccaaag gcaccagtac gcgccccacg
    13561 ctgacggttt ctaacctgta cggtatggtc accgggatgg cggaagatct gcagagtctg
    13621 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
    13681 gtcaacggaa acagtgacgc cgatccggag caggaggtga tcagccgctg gcgcatcgag
    13741 cagtgcagcg aactgagcgc ggtgagtgcc tcctttgtac tgtccacgcc gacggaaacg
    13801 gatggcgctg tttttccggg gcgcatcatg ctggctaaca cctgcacctg gatctatcgc
    13861 ggtgatgagt gcggttataa tggcccagct gtcgctgatg aatatgacca gccgacatct
    13921 gatatctcga aggataaatg cagtaaatgc ctgagcggct gtaagttccg caataacgtc
    13981 ggcaactttg gcggcttcct ttccattaac aaactttcgc agtaaatccc atgacagaga
    14041 cagaatcagc gattctggcg cacgcccggc gatgtgcgcc agcggagtcg tgcggcttcg
    14101 tggtgagaac gccggagggg gaaagatatt ttccctgcgt aaatatctcc ggtgagccgg
    14161 aggattattt tcggatgtcg ccggaggact ggctgcaggc agaaatgcag ggtgagattg
    14221 tggcgctggt ccacagtcat cccggtggtc tgccctggct gagtgaggct gaccggcggc
    14281 tgcaggtgca gagtgatttg ccgtggtggc tggtctgccg gggggcgatt cataagttcc
    14341 gctgtgtgcc gcatctcacc gggcggcgct ttgagcacgg ggtgacggac tgttacacgc
    14401 tgttccggga tgcttatcat ctggcgggga ttgagatgcc gaattttcat cgcggggatg
    14461 actggtggcg tcacggccag aatctctatc tggataatct ggaggacaca gggctgtatc
    14521 aggtgccgtt gtcatcagca caaccgggcg atgtgctgct gtgctgtttt ggttcatcgg
    14581 tgccgaatca tgccgccatt tactgtggtg acggcgagct gctgcaccat attcctgaac
    14641 aactgagcaa acgagagagg tataccgaca aatggcagcg acgcacacac tccctctggc
    14701 gtcaccgggc atggcgcgca tctgccttta cggggattta caacgatttg gccgccgcat
    14761 cgaccttcgt gtgaaaacgg gggctgaagc catccgggcg ctggccacac agctcccggc
    14821 gtttcgtcag aaactgagcg acggctggta tcaggtacgg atttccgggc gggacgtcag
    14881 cacgtccgga ttgacggcgc agttacatga ggttctgcct gacggcgctg tgattcatat
    14941 tgttcccaga gtcgccgggg ccaagtcagg gggcgtattc cagattgttc tgggggctgc
    15001 cgccattgcc ggatcattct ttactgccgg agccaccctt gcagcatggg gggcagccat
    15061 tggggccggt ggtatgacca gtatcctgtt ttctctcggt gccagtatgg tacttggtgg
    15121 tgtggcgcag atgctggcac cgaaagccag gacgcccacg gcagcaagta cagataacgg
    15181 caaacagaac acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc
    15241 cgttctgtac ggtgaaatgc gcgtggggtc gcgggtggtc tctcaggaga tcagcacggc
    15301 agacgaaggg gatggtggtc aggttgtggt gattggtcgc tgatgcaaaa tgttttatgt
    15361 gaaaccgcct ccgggcggtt ttatcgttta tggagcatga cgaatgggta aaggcggcag
    15421 taaggggcat accccgcgcg aagcgaagga caacctgaag tccacgcagc tgctgagtgt
    15481 gatcgatgcc atcagcgaag ggccggttga aggtccggtg gatggattaa aaagcgtgct
    15541 gctgaacagt acgccggtgc tggacagtga ggggaatacc aatatatccg gcgtcacggt
    15601 ggtgttccgg gccggtgagc aggagcagac accgccggag ggatttgaat cctccggctc
    15661 cgagacggtg ctcggtacag aagtgaaata tgacacgccg atcacccgga ccatcacgtc
    15721 ggcaaacatt gaccgtctgc gttttacttt cggcgtgcag gcactggtgg aaaccacctc
    15781 aaagggggac aggaatccat cggaagtccg cctgctggtt cagatacaac gtaacggtgg
    15841 ctgggtgacg gaaaaggaca tcaccattaa gggtaaaacc acttcacagt atctggcctc
    15901 ggtggtggtg gataacctgc cgccgcgccc gttcagtatc cggatgcgca ggatgacgcc
    15961 ggacagcacc acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaattat
    16021 cgatgtgaaa cagtgctacc cgaacacggc actggtcggc gtgcaggtgg actcggagca
    16081 gttcggcagc cagcaggtga gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc
    16141 gtcgaattat aacccgcaga cgcggcaata cagcggtatc tgggacggaa cgtttaaacc
    16201 ggcatacagc aacaacatgg cctggtgtct gtgggatatg ctgacccatc cgcgctacgg
    16261 catggggaaa cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg tcatcggcca
    16321 gtactgcgac cagtcagtgc cggacggctt tggcggcacg gagccgcgca tcacctgtaa
    16381 tgcctacctg accacacagc gtaaggcgtg ggatgttctc agcgatttct gctcggcgat
    16441 gcgctgtatg ccggtatgga acgggcagac gctgacgttc gtgcaggacc gaccgtcgga
    16501 taaggtgtgg acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg
    16561 ctacagcttc agcgccctca aggaccgcca taatgccgtt gaggtgaact ggattgaccc
    16621 ggataacggc tgggagacgg cgacagagct tgtggaggac acgcaggcca ttctccgtta
    16681 cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc agccgggggc aggcacaccg
    16741 cgccgggctg tggctgatta aaacggagct gctggagacg cagaccgtgg atttcagcgt
    16801 gggtgccgaa gggcttcgcc atgtgccggg tgatgttatt gaaatctgcg atgatgacta
    16861 tgcggggatc agcatcggcg gacgcgtgct ggcggtgaac agccagaccc ggacgctgac
    16921 gctcgaccgt gaaatcacgc tgccatcctc cggtaccacg ctgataagcc tggttgacgg
    16981 aagtggcaat ccggtcagcg tggaggtcca gtccgtcacc gacggcgtga aggtaaaagt
    17041 gagccgtgtt cctgacggcg ttgccggata cagcgtatgg gggctgaagt tgccgacgtt
    17101 gcgccagcgc ctgttccgct gcgtgagtat ccgtgagaac gacgacggca cgtatgccat
    17161 taccgccgtg cagcatgtac ccgaaaaaga agccatcgtg gataacgggg cgcactttga
    17221 cggcgacttg agcggcacgg tgaatggcgt cacgccgccc gcggtgcagc acctgactgt
    17281 cgaagtcacc gcagacagcg gggaatatca ggtgctggcg cgctgggaca cgccgaaggt
    17341 ggtgaagggg gtgagcttcc tgcttcgcct gaccgtggca gcggacgatg gcagtgagcg
    17401 gctggtcagt acggcaagga cgacggaaac cacataccgc ttcacgcaac tggcgctggg
    17461 gaactacagg ctgactgtcc gggcggtaaa tgcgtgggga cagcagggcg atccggcatc
    17521 ggtatcgttc cggattgccg caccggcagc gccgtctcgg attgagctga caccgggcta
    17581 ttttcagata accgccacgc cgcatcttgc ggtttatgat ccgacggtac aatttgagtt
    17641 ctggttctcg gaaacgcgga ttaccgatat caggcaggtt gaaaccacag cccgctacct
    17701 tggcacgggg ctgtactgga tagccgccag tatcaatatc aaaccgggcc atgattatta
    17761 cttttatatc cgcagtgtga acaccgttgg caaatcggca ttcgtggagt ccgtcggtcg
    17821 ggcgagcgat gatgcggaag gttacctgga ttttttcaaa ggcaagataa ccgaatccca
    17881 tctcggtaaa gagctgctgg aaaaagtcga tctgacggag gataacgcca gcagactgga
    17941 tgagttttcg aaagagtgga aggatgccaa cgataaatgg aatgccatgt gggctgtcaa
    18001 aattgagcag accaaagacg gcaaacatta tgtcgcgggt attggcctca gtatggagga
    18061 cacggaggaa ggcaaactga gccagtttct ggttgccgct aaccgtatcg cgtttattga
    18121 cccggcaaac gggaatgaaa cgccgatgtt tgtggcgcag ggcaaccaga tattcatgaa
    18181 cgacgtgttc ctgaaacgcc tgacggcacc caccattacc agcggtggca gtcctccggt
    18241 attttccctg acatcagacg gaaagctgac cgctaaaaat gcggatatca gtggcagtgt
    18301 gaatgcgaac tcagggacgc tcaacaacgt cacgattaat gagaactgtc agattaaggg
    18361 gaaactgtca gccaaccaga ttgaaggcga tattgtcaaa acggtcagca agtctttccc
    18421 ccgcacgaac agttatgcca gcggcaccat cacggtaaga atcagtgatg atcagaaatt
    18481 tgaccggcag gtcatgatac cgccagtgtt attccgcggt ggtaagcatg agaatttcaa
    18541 cagtaataac caacagtcat actggtattc aacctgccgg ttaagagtga cccgcaatgg
    18601 tcaggagatt tttaatcagt ccacgacgga tgctcagggc gtattttctt cagttataga
    18661 tatgcctgcc ggacagggga cactgacact gacattcacc gtatcttcat caggagcgaa
    18721 taactggaca ccaacaacca gtatcagcga tctgctggtt gtggtgatga agaaatccac
    18781 agcaggtatc agtatcagct gaattttata acccataacg ggcgtcagaa atgacgcctt
    18841 ttttattgca gaaaagcgag aggtaattat gcgtaaactt tatgccgcca ttttgtccgc
    18901 agccatttgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
    18961 gagcgcgggg tatcttcatg cctcgacgaa cgttcccggc agcgatgatc tgaacgggat
    19021 taacgtgaaa taccgttatg agtttacgga cacactgggg ctggtgacgt cattcagcta
    19081 tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc atgaagattc
    19141 cgttcgtaac cgctggttca gcgtgatggt ggggccgtct gtgcgcgtga atgaatggtt
    19201 cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgactttct ccggggatta
    19261 tctccgcgta actgacaaca aggggaaaac gcacgacgtg ctgaccggaa gtgatgacgg
    19321 tcgccacagc aacacgtctc tggcgtgggg agctggcgtg cagtttaacc cgaccgaatc
    19381 cgtggccatt gatattgctt atgaaggctc cggcagtggc gactggcgca ctgacggttt
    19441 catcatgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgacagcccg
    19501 ccggttcagg cgggcttttt tgtggggtga atatggcagt aaagatttca ggtgtactga
    19561 aagacggcac aggaaaaccg gtacagaact gcacaatcca gctgaaagca aaacgtaaca
    19621 gcaccacggt ggtggtgaac acgctggcct cagaaaatcc ggatgaagcc gggcgttaca
    19681 gcatggacgt tgagtacggt cagtacagcg ttattctgtt ggtggaagga ttcccgccgt
    19741 cacatgccgg gaccatcacc gtgtatgaag attcccgacc cggtacgctg aatgattttc
    19801 tcggtgccat gacggaggat gatgcccgtc ctgaggcact gcgccgtttt gaactgatgg
    19861 tggaagaggt agcgcgtaac gcgtccgtgg tggcacagaa cacggcagcc gcgaagaagt
    19921 cagccagtga tgccagcaca tctgcccgtg aggcggcaac ccatgcgact gatgctgcag
    19981 attcagcacg tgcagccagc acgtcagccg gacaggccgc ttcgtcggct cagtcagcgt
    20041 cttccagcgc aggaacggca tcaacaaagg ccactgaagc ctcaaaaagt gctgccgctg
    20101 cagagtcctc aaaaagcgtg gcagctacca gtgccggtgc ggcgaaaacg tcagaaacga
    20161 atgccgcagc gtcagaacaa tcagccgcca cttctgcatc caccgcgatc acgaaagcgt
    20221 cagaagctgc cacctcagcc cgggatgcgg cggcctcaaa agaggcagcg aaatcatcag
    20281 aaacgaacgc atccttgagc gccagtagcg cagcttcctc ggcaacggcg gcaggaaatt
    20341 ccgcgaaggc ggcaaaaacg tccgagacga acgccaggtc ttctgagacg gcagcgggac
    20401 agagcgcctc agctgcggca ggctcaaaaa cagcggctgc gtcgtctgcc agtgccgcgt
    20461 caacaagtgc cgggcaggcc tcagccagtg ccaccgccgc cggaaaatcg gcagaaagcg
    20521 ccgcatcatc cgcttcaaca gccacaacga aggctggcga agccactgag caggccacgg
    20581 cagcagcgag gtcagcttcc gcagcgaaga catccgaaac gaacgcgaaa gcgtcggaaa
    20641 cccgtgcaga atcctcaaaa acggcagcgg catcgtccgc cagttcggcg gcgtcatcgg
    20701 catcatcggc gtctgcttca aaagatgagg cgaccagaca ggcgtcagca gcgaagggca
    20761 gcgccacgac ggcatccaca aaggcgacag aggcagctgg tagtgcgacg gcagcagctc
    20821 agagcaaaag tacggcggaa tctgcagcaa cgcgcgctga gacagcggca aaacgggcag
    20881 aggatattgc atccgccgtg gcgcttgagg atgcgagcac gacgaaaaag gggatagtac
    20941 agctcagcag tgcgaccaac agcacttccg agtcactggc ggcaacgcca aaagcagtta
    21001 aggccgcgta tgacctggct aacgggaaat acacagcaca ggatgcaacg acagcacaga
    21061 aagggatagt ccaactaagc agcacgacca acagcacgtc tgaaacgctg gcggcaacgc
    21121 caaagtcggt aaaggcagcc tatgaccttg ctaacgggaa atataccgct caggacgcta
    21181 cgacagcaca aaaaggaatt gtccagctca gtagtgcaac caacagcgca tctgaaacgc
    21241 ttgccgcgac accgaaagca gtgaaagcag ctaatgataa tgcgaatggt cgggtacctt
    21301 ctgcccgtaa ggtgaatggt aaggcgcttt cagcggatat aacactgacg ccgaaagata
    21361 ttggtacgct taactcaaca acaatgtcat tcagcggtgg tgctggttgg ttcaaattag
    21421 caacggtaac catgcc
//
LOCUS       DAZFFI010000078        20641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFFI010000078 DAZFFI010000000
VERSION     DAZFFI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.078"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..1121
                     /gene="phoE"
                     /locus_tag="ACHJ12_003377"
     CDS             66..1121
                     /gene="phoE"
                     /locus_tag="ACHJ12_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4991115.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1160..1561)
                     /gene="crl"
                     /locus_tag="ACHJ12_003378"
     CDS             complement(1160..1561)
                     /gene="crl"
                     /locus_tag="ACHJ12_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4991116.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1619..2863)
                     /gene="frsA"
                     /locus_tag="ACHJ12_003379"
     CDS             complement(1619..2863)
                     /gene="frsA"
                     /locus_tag="ACHJ12_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4991117.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2955..3413)
                     /gene="gpt"
                     /locus_tag="ACHJ12_003380"
     CDS             complement(2955..3413)
                     /gene="gpt"
                     /locus_tag="ACHJ12_003380"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4991118.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3674..5131
                     /gene="pepD"
                     /locus_tag="ACHJ12_003381"
     CDS             3674..5131
                     /gene="pepD"
                     /locus_tag="ACHJ12_003381"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4991119.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5188..5802)
                     /gene="prfH"
                     /locus_tag="ACHJ12_003382"
     CDS             complement(5188..5802)
                     /gene="prfH"
                     /locus_tag="ACHJ12_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4991120.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5799..6938)
                     /locus_tag="ACHJ12_003383"
     CDS             complement(5799..6938)
                     /locus_tag="ACHJ12_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4991121.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7184..7636)
                     /gene="yafP"
                     /locus_tag="ACHJ12_003384"
     CDS             complement(7184..7636)
                     /gene="yafP"
                     /locus_tag="ACHJ12_003384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4991122.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRGAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7633..8688)
                     /gene="dinB"
                     /locus_tag="ACHJ12_003385"
     CDS             complement(7633..8688)
                     /gene="dinB"
                     /locus_tag="ACHJ12_003385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4991123.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8759..9544)
                     /gene="lafU"
                     /locus_tag="ACHJ12_003386"
     CDS             complement(8759..9544)
                     /gene="lafU"
                     /locus_tag="ACHJ12_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4991124.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9516..11228
                     /locus_tag="ACHJ12_003387"
                     /pseudo
     CDS             9516..11228
                     /locus_tag="ACHJ12_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11333..11611
                     /locus_tag="ACHJ12_003388"
     CDS             11333..11611
                     /locus_tag="ACHJ12_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4991125.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            11604..11960
                     /locus_tag="ACHJ12_003389"
     CDS             11604..11960
                     /locus_tag="ACHJ12_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4991126.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(12017..12790)
                     /gene="yafL"
                     /locus_tag="ACHJ12_003390"
     CDS             complement(12017..12790)
                     /gene="yafL"
                     /locus_tag="ACHJ12_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4991127.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12976..13236
                     /gene="dinJ"
                     /locus_tag="ACHJ12_003391"
     CDS             12976..13236
                     /gene="dinJ"
                     /locus_tag="ACHJ12_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4991128.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            13239..13517
                     /gene="yafQ"
                     /locus_tag="ACHJ12_003392"
     CDS             13239..13517
                     /gene="yafQ"
                     /locus_tag="ACHJ12_003392"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO4991129.1"
                     /translation="MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            13673..14413
                     /gene="dpaA"
                     /locus_tag="ACHJ12_003393"
     CDS             13673..14413
                     /gene="dpaA"
                     /locus_tag="ACHJ12_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4991130.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14384..15151)
                     /gene="yafJ"
                     /locus_tag="ACHJ12_003394"
     CDS             complement(14384..15151)
                     /gene="yafJ"
                     /locus_tag="ACHJ12_003394"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4991131.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(15357..15935)
                     /gene="lpcA"
                     /locus_tag="ACHJ12_003395"
     CDS             complement(15357..15935)
                     /gene="lpcA"
                     /locus_tag="ACHJ12_003395"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4991132.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16175..18619
                     /gene="fadE"
                     /locus_tag="ACHJ12_003396"
     CDS             16175..18619
                     /gene="fadE"
                     /locus_tag="ACHJ12_003396"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4991133.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLAGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(18662..19108)
                     /gene="ivy"
                     /locus_tag="ACHJ12_003397"
     CDS             complement(18662..19108)
                     /gene="ivy"
                     /locus_tag="ACHJ12_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4991134.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19289..20059
                     /gene="yafV"
                     /locus_tag="ACHJ12_003398"
     CDS             19289..20059
                     /gene="yafV"
                     /locus_tag="ACHJ12_003398"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4991135.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(20101..>20641)
                     /locus_tag="ACHJ12_003399"
     CDS             complement(20101..>20641)
                     /locus_tag="ACHJ12_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4991136.1"
                     /translation="GRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDV
                     PDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRN
                     HWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMR
                     KAAMDRNYLASVLAESGLS"
BASE COUNT         5043 a         5258 c         5280 g         5060 t
ORIGIN      
        1 ctttcctgtt ttcaacgggg ctctcccgct gaatattcgc gcgttaatta aaatcaggaa
       61 tgaaaatgaa aaagagcact ctggcattag tggtgatggg cattgtggca tctgcatctg
      121 tacaggctgc agaaatatat aataaagacg gtaataaact ggatgtctat ggcaaagtta
      181 aagccatgca ttatatgagt gataacgcca gtaaagatgg cgaccagagt tatatccgtt
      241 ttggttttaa aggtgaaaca caaattaacg atcaactgac tggttatggt cggtgggaag
      301 cggagtttgc cggaaataaa gcggagagtg ataccgcaca gcaaaaaacg cgtctcgctt
      361 ttgccgggtt gaaatataaa gatttgggtt ctttcgatta tggtcgtaac ctgggcgcgt
      421 tgtatgacgt ggaagcctgg accgatatgt tcccggaatt tggtggcgat tcctcggcgc
      481 agaccgacaa ctttatgacc aaacgcgcca gcggtctggc gacgtatcgg aacaccgact
      541 tcttcggcgt tatcgatggc ctgaacttaa ccctgcaata tcaagggaaa aacgaaaacc
      601 gcgacgttaa aaagcaaaac ggcgatggct tcggcacgtc attgacatat gactttggcg
      661 gcagcgattt cgccattagt ggggcctata ccaactcaga tcgcaccaac gagcagaacc
      721 tgcaaagccg tggcacaggc aagcgtgcag aagcatgggc tacaggtctg aaatacgatg
      781 ccaataatat ttatctggca actttttatt ctgaaacacg caaaatgacg ccaataactg
      841 gcggctttgc caataagaca cagaactttg aagcggtcgc tcaataccag tttgactttg
      901 gtctgcgtcc atcgctgggt tatgtcttat cgaaagggaa agatattgaa ggtatcggtg
      961 atgaagatct ggtcaattat atcgatgtcg gggctacata ttatttcaac aaaaatatgt
     1021 cagcgtttgt tgattataaa atcaaccaac tggatagcga taacaaattg aatattaata
     1081 atgatgatat tgtcgcggtt ggcatgacct atcagtttta atagatattg ccggatgtga
     1141 tgcatccggc acatttcact cacgccgtta acttcaccgg ctcgtcacga aaatcatccg
     1201 ccggttccag cttcagattc agcgtcgtca gcagttcacg cagcttctcg tgaaactcac
     1261 gcagggtgtg ctccagtcgt tcaaccactt cagtatcttt taccggaaca ctcttccagt
     1321 cgcctgcttt atcgaacaga ccaaactggt agctgtaggt aaaacgggat tcctgcgctt
     1381 ccagctccat ccaccagccc cagaactcac gcacttccgg tgccggtttc acgttgacgc
     1441 atacagccag acaatcgaaa aagaatcgat tatcttcgca cttaccttca cgaatatacg
     1501 ggcctagtgc ggtaaatttt ttgatcaatc tgctcttcgg gtgtccactc ggtaacgtca
     1561 ttgcaatctc ctgttgtgat gcaactgttt taccaaattg gtaaaattta gcaaattttt
     1621 aacacaaacg tttttcgatc cagtcggtga tttcctgaag acctttgtca aaattccgat
     1681 acaccgggtt aaatgggatc tctaataatt taccgtcagc agatgatgag gtgattaagc
     1741 gtgagtcctc ttccgggctg aacggatcgt tcttccagta gcctgataac attggcgttg
     1801 ggcagcgacg tcccagcaat ccttgcactt ttaatgaata gcgattcaac tccacgcgca
     1861 acgcttcgtc ggaagcatca tgcatcccca aacgactcgc cagaacgtca agatacattt
     1921 ccggcacctg ttgctggcac ttaaaatcac tcaacagggt atgaactacc ggaccaagac
     1981 aggcaaccgc tttcagacgc ggcgattcaa ggtatgccag acgcacggca acgttagcgc
     2041 cgaaacggaa accaaaggcc gcgacgcgag tgtgatccac ccacggtacg ttaggcagcg
     2101 cctttaagac gtgctgatgc aacaggctgg agtcctgggt gagcttccat tttgaagaaa
     2161 agcccaccga cggcatatca atagtcagca tcgcaatgcc gcgcggcgca aaataacgtt
     2221 catacaggct gtaatagtcc gtctgcatcg catccagacc accacacatt aatactgtcg
     2281 ggaacgggcc atcgcctttc ggcatatgca aaaagccggt gatgggcgca ccgccgggta
     2341 cggtaaactc catctgccgc atcgtgcccg gcagacgctg ggcggcctct tcataggcgc
     2401 ggtttgataa agcctgcgct tgctcggcca ggtcatctcc tttcagatga ggataggcgg
     2461 caatgttgta caacgtagcc gcatgcagcc agtgtcgacc actgagttgc ggatcttctt
     2521 ccgcacaggc tttttgttgc cacaccatcg cctgggtggc ccactcataa atccagttgc
     2581 caccacggta gccaatcacg gtatcgtata aatcatcatc ggtacgttca gcatcgctca
     2641 tcacaatacg tgcctggacg tcgaggattt cacgtgggtc aatgccgcgc cagatccaca
     2701 tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaaga gccgactgca
     2761 caggcggttg tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca ggatgtttaa
     2821 agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca gcctccagaa
     2881 tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc cggcagtgcc
     2941 aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc catatcccac
     3001 ggctgttcga tccaggtatc ttgcgggata tcaacaacat agtcatcaac cagcggacga
     3061 ccagccggtt ttgcgaagat agtcacaaaa tgtgcttttg gatacatttc acgaatcgca
     3121 accgcagtac caccggtatc caccaggtca tcaataacga tgaagccttc gccatcgcct
     3181 tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct ggaaatacaa
     3241 acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg taccagaccg
     3301 ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt
     3361 ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt
     3421 gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga
     3481 ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca
     3541 ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt
     3601 gtgttgacaa cattttctgc taaccctgtg acctgcaata ctgttttgcg ggtgatcgac
     3661 aaggagactt aacgtgtctg aactgtctca attatctcca cagccgctgt gggatatttt
     3721 tgccaaaatc tgttctattc ctcacccgtc ctatcatgaa gagcaactcg ctgaatacat
     3781 tgttggttgg gcaaaagaga aaggtttcca tgtcgaacgc gatcaggtag gtaatatcct
     3841 gattcgtaaa cctgctaccg caggtatgga aaatcgtaaa ccggtcgtct tacaggccca
     3901 cctcgatatg gtgccgcaga aaaataacga caccgtgcat gacttcacga aagatcctat
     3961 ccagccttat attgatggcg aatgggttaa agcgcgcggc accacgctgg gtgcagataa
     4021 cggcattggt atggcgtctg cgctggcggt tctggctgac gaaaacgtgg ttcacggccc
     4081 gctggaagtg ctgctgacca tgaccgaaga agccggtatg gacggtgcgt tcggcttaca
     4141 gagcaactgg ttgcaggctg atattctgat taacaccgac tccgaagaag aaggtgaaat
     4201 ctacatgggt tgtgcggggg gtatcgactt cacctccaac ctgcatttag atcgtgaagc
     4261 ggttccagct ggttttgaaa ccttcaagtt aaccttaaaa ggtctaaaag gcggtcactc
     4321 cggcggggaa atccacgttg gcctgggtaa tgccaacaaa ctgctggtgc gcttcctggc
     4381 gggtcatgca gaagaactgg atctgcgcct tatcgatttc aacggcggca cactgcgtaa
     4441 cgccatcccg cgtgaagcct ttgcgaccat tgctgtcgca gctgataaag tcgacgtcct
     4501 gaaatctctg gtgaatacct atcaggagat cctgaaaaac gagctggcag agaaagagaa
     4561 aaatctggcc ttgttgctgg actctgtagc gaacgataaa gctgccctga ttgcgaaatc
     4621 tcgcgatacc tttattcgtc tgctgaacgc caccccgaac ggtgtgatcc gcaactcaga
     4681 cgtggcaaaa ggtgtggtcg aaacctccct gaacgtcggt gtggtgacca tgactgataa
     4741 taacgtagaa attcactgtc tgatccgttc actgatcgac agcggtaaag actacgtcgt
     4801 gagcatgctg gattcgctgg gtaaactggc tggcgcgaaa accgaagcga aaggcgcata
     4861 tcctggctgg cagccggacg ctaattctcc ggtgatgcat ctggtacgtg aaacctatca
     4921 gcgtctgttc aacaaaacgc cgaacatcca gattatccac gcgggcctgg aatgtggtct
     4981 gttcaaaaaa ccgtatccgg aaatggacat ggtttctatc gggccaacta tcaccggtcc
     5041 acactctccg gatgagcaag ttcacatcga aagcgtaggt cattactgga cactgctgac
     5101 tgaactgctg aaagaaattc cggcgaagta attatttgat ttgctgccgg atggcgttta
     5161 atcgccttcc ggcagtttca tccttcatta tccttcgata aaagccatcc ctgtaaatgt
     5221 ccgtctcggg ttgccacgtt caatctggtg atggaacatt cgccgctgcg atttcagcac
     5281 cgcgctattt tcctgttgct gctgctccag cttccaggca atcagcaatc gtgccagccg
     5341 cttgttagca tgctgactac gctctgactg aaccttcacg ctaataccgg atgccaaatg
     5401 cgtggcgcgt accgccgagt cggttttatt gacatgttga ccgcccggcc ccgacgaacg
     5461 caacgtctca taacggattg catccgattg ttcctgctca tcagcggtaa aacgcccaat
     5521 gcccagaaac cagtttttgc gcccatgatg aggccgatac ggactcggac aaatccactg
     5581 aatagtgcca caccagcttt cgcttaatgc ccatgcgtta tcgccatcca gagaaatcag
     5641 cgccgaacgc agcgtgtcag agtagcggcc cgtttctgtt tccaacaccg ttaccgcgac
     5701 gtcttgtcgg gcagcttctt taattagcct gtccagcgcc tttttcactg cgagacaaca
     5761 ttcttccggc ccctgcgctg aggagagttg tagcaagatc atcctttttt ccctccactg
     5821 tttttgagcg ttagcaccgg acgcagccgc gcgacaggaa taattaaacc ggcctgcacc
     5881 agacattgca ccacgctttc agccgattta taggcttgtg gcgcttcttc aaagatgagt
     5941 tgtttatcgc gacaaattac ccgactgcca agttcagtac gcgagagttg cgtcgccgtg
     6001 tatttcgctg ccagacgccc tttacactcg gtgcgccccc atttacgccc tgccccatgc
     6061 gccagcgagt ggagcgtttt ttcgttcgca acaggctgta caatccagga gtaatcacca
     6121 cgtgaaccgg gaataatcac cagaccgttg tcatccggcg tggcaccttt acgatgcaac
     6181 cagccctgtt gatcgccgat ttgacacgcg ctaacaaagt tatgcgccac atccagaacc
     6241 ggacttcccg tggccttaac ttgttgcatt atgcgcaaag cgatcatctg gcgattaata
     6301 cacgcaaacg ccagcgcatc gtcatgttcc gcaatatacc gtagcgcgtc gtcactgcct
     6361 tgaggcaaac catgatgcga gaacgaggca atatgccgct gtaaaataga ctgccccacc
     6421 ccccgcgagc cgctatgaac caacagttgc agatgttgtg catccagacc tgccagtgca
     6481 aacagttcag cgttgatgat ttgatcaact tgttgcagtt cagcgaaatg attgccgcca
     6541 ccgatagagc caagcgagct gcgccacgga tgctgtgcaa atgctgacgg caggttttcc
     6601 tccagccagc tttcttcagc aacgtcatcc agatctgata atcgcttttc aaacttatcg
     6661 gcgttgtatt tgcgagtgag aatatctgtt tgccatagcg ccataccgca gccgatatca
     6721 ttgccgacca gtgccggata aaaacgacca acagagaaga acgctgcgcc aatcggatag
     6781 ccgcgtccgg gatgtaaatc tggcatcccc actacgcgct gcatgttggg taaatttgcc
     6841 gtggtgtgta attgttggat cgctaaactc tcgatccaca ggtcatcaga tgcgatggta
     6901 aataccgcat cggataaggg acgaatataa ttgcccatat accattccgt aaatagactc
     6961 gtcatacttc aagttgcatg tgttgcgact tgaattattg agagtaacaa ttaaaaaatc
     7021 aggaaatggc agacgaatcc agggcaagaa aaaaagaatt aatcctggaa aggtctgcaa
     7081 agagtcattg ataaaggatt tgtcatgatt tacctccttg cagtttgtgg aataagaaaa
     7141 cgcgcacata ctaggcttgc gttattttca ttgcaagctg gatttaatgt tgctgtttat
     7201 aacgcatata aaaattaata aaccactctc cccgacattc aacgcgttgc tgcttaactg
     7261 tctgaaaacc ataacgttca aaaaagggtt ttgcggttat gcttgcgtcc accgtaagtt
     7321 cggattcaga cttaatcaaa ggttttaaca aagcgctggc aaccccacgg cgggtgtatt
     7381 cagggtcaac aaataacata tcgatatagt gttcaacgca ggtaataaag ccgaccagtt
     7441 ttgcattaat gactgcaccc cgcacttgtg atttcgcgag tttctccttc cagcgagatt
     7501 cgtcaatctg cgcccaggcg gcaatttgtt gtggtgaata atgctgactg gcagtcatcg
     7561 taaccgctct aatgaaaata gcgcatagtt gctgaaaatc gccaggctga tagtctctta
     7621 tttgtatgtt attcataatc ccagcaccag ttgtctttcc atttgcgggt caagcaacgt
     7681 cacatgcagc cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc aggttttacg
     7741 cgcggtggcg attaagtcag ctttattcag ccgcggccag acgtgctcct gagtggtttg
     7801 ctgaaaatcg tcgaacttta atttcacccc ctggcgggca atcagtaaat caggttttac
     7861 ctttgccaga cggcgttcaa gttccggata cagccgctcg ataatcgctt cacattcaga
     7921 ccagtggtgg atatcttccg ccatcgtgcg ttccacgccg acggattttc gcaaccgttc
     7981 gctgttaacg tcgcgctcgt caatcccctg actacgctcc cacaaaatgc ggccaaattt
     8041 gccaaagcgt ttaagcagta tcaccagatc acacttttgt acatcaccgc aggtccgcag
     8101 ccccatcgct tccagttttg ccgccgagac tttgccgacg ccggggattt ttgccagcgg
     8161 taaggtttgt aaaaatgccg gaacttctgc cggcgtaatc acaaactgac cgttgggttt
     8221 attcatgtcg gaggcgattt tggcgagaaa ctttactggt gccacgcccg cagacgctgt
     8281 cagttgcagc tcgctgaaaa tcgtctggcg gatttcctgg gcgatgaggg tcgcagaacc
     8341 gtggcaatgg acgctatcgg tgacgtcgag ataagcctca tccagtgaca atggttcaat
     8401 acgcgaggtg tagcgcgaga agatttcacg gatatgattt gaggcttctt tgtaggcgtc
     8461 aaagcgcccc ggaagcaagg tgagatgcgg gcataatttg agcgccatcc ctgtcggcat
     8521 agcgctacgt acgccaaatt tacgcgcggg ataattggcg gtgctgatca ccccccgacg
     8581 ttcgcggctg ccgccaatag caatagggat atcgcgcagg gcgggattgt cgcgcatctc
     8641 caccgctgcg aaaaagcagt ccatatccac atgaatgatt ttacgcatta ctcacctctc
     8701 aacactggta aagtatacag tgatttcagg gtttgagaaa tgcgtaaaga ttcagcattt
     8761 aacgcgttcg ctgcgaaagc acctgctgct tatccagctt ttgcaccagc ggctgcacca
     8821 ctttatcccc atgctgacca aagtattgat acagcgtatc ggacgcactt ttggtcagca
     8881 ccataatctc aatgcgccgg ttgcccgcgc tttgcggatt tttggaatcc agcagcatct
     8941 ggtccgccat tgcgcttacc tgcatcactt tatcttccgg cattccggcc tcttccagca
     9001 cccgacgagc cgaaagcgcg cggtcacccg aaaggttcca gttgttgtag atattgtttt
     9061 tgtaagccat cgcatcggta tgcccggtaa taataatttt gttatcgagc gagtcgaaaa
     9121 ctggcgcgag ctccaccagc agcgttttaa agaacggcat aatctgcgcg ctgccgcgtt
     9181 cgaacatatt gcgattctgg tcgtctttaa tcagcacgcg taatccctgg ggaacaatct
     9241 ccatctccag attcgcttcc atatgcgcat cacgggcaat ggtggtgata ctggttgcca
     9301 gctcgccaag ctcggtggcg gatttcttct tcagcagcgc cgttttctca ttcacatcac
     9361 gcgctttttt ctccgtttct tccggcaccg ccacggtggc aggttttggc ggatgagacg
     9421 ggctgatttt atttaacggc gacagcccac caccgttaaa aatcgactgc ccgtgcagcg
     9481 cggcgataat gctttcacgc tcggatttac tgacgctgtt gacaatcaac tttattgtcg
     9541 tcaccaaagg ggccgagcgt atttccgagg tttcagcccg ctttaccctg gatgcgatgc
     9601 ccggtaagca gatggcgatt gacgccgatc ttaatgccgg gttgatcaat caggcgcagg
     9661 cacaaacccg acgtaaagat gttgccagcg aggccgattt ctacggcgcg atggacgggg
     9721 catcgaaatt tgtgcgcggg gatgctatcg ccgggatgat gattctggcg atcaacctga
     9781 tcggcggcgt ctgtatcggg atcttcaaat acaacctgag cgccgacgcc gccttccagc
     9841 agtatgtgct gatgaccatc ggcgacgggc tggtggcgca aatcccttcc ctgctactct
     9901 ccaccgcggc ggcgattatc gtcacccgcg tcagcgacaa cggcgatatc gcccatgacg
     9961 tgcgtcacca gttgctggca agcccatcgg tgctctacac cgctaccgga attatgttcg
    10021 tactggcggt agtgcctgga atgccacatc tgccgttttt gctgttcagc gccctgcttg
    10081 gctttaccgg ctggcggatg agcaaacgcc cgcaggcggc ggaagcggaa gagaaaagtc
    10141 tcgaaacgct gacccgcacc atcaccgaaa ccagcgagca acaggtcagt tgggaaacca
    10201 ttccgctgat cgagcccatc agcttaagcc tcggctacaa gctggtggcg ctggtggaca
    10261 aagcccaggg caacccgctc acccagcgta ttcgcggcgt gcgacaggtg atatccgacg
    10321 gcaacggcgt gctgctgccg gagatccgca ttcgggaaaa cttccgcctt aagcccagcc
    10381 agtacgctat tttcatcaac ggcattaagg ctgatgaagc ggatattccg gcggataaac
    10441 tgatggcgct gccctccagc gaaacctacg gcgagataga cggcgtgctg gggaacgacc
    10501 cggcgtacgg gatgccggtg acctggatcc agcctgcgca gaaagcgaag gcgctgaata
    10561 tggggtatca ggtgatcgac agcgccagcg tgattgctac ccatgtaaat aagattgtgc
    10621 gcagctatat tcctgattta tttaactatg acgatattac gcagttgcat aaccgtttgg
    10681 cgtcgatggc accgcgcctg gcggaggatt taagcgcggt gctcaattac agccagttgc
    10741 tgaaagtgta ccgggcgctg ctgaccgaag gcgtttccct gcgcgatatc gtcaccatcg
    10801 ccaccgtgct ggtcgccagt agcgcggtga ccaaagatca tattctgctg gcagccgatg
    10861 tgcgcctggc gctgcggcgc agcattaccc atccgttcgt tcgcaagcag gagctgacgg
    10921 tgtataccct gaataatgag ctggaaaatc tgctgactaa cctagtgaat caggcgcaac
    10981 agggcggtaa agtgatgctc gacagcgtgc cagtggaccc gaatatgctc aaccagttcc
    11041 agagtacgat gccacaggtg aaagagcaga tgaaagcggc ggggaaagac ccggtgctgc
    11101 tggtgccacc gcagctgcgc cctttgctgg cgcgttatgc aaggttgttt gcgccggggc
    11161 tgcatgtgct gtcgtataac gaagtgccgg atgagctgga gttgaagatt atgggggcgt
    11221 tgagttgatg ggggattctt ttccatgctt tgcagcacct gacaccatta attgacgtca
    11281 cactgcaaag agactacgat gacaccatac aatgacgtca tggattgata gtatgattaa
    11341 ggtcagcgcg ctaggttctc gtcatagaaa aacattcgac gatgtaatga cggagccgcc
    11401 aaaatctgga attaaatggg atgatgttgt tgcactcatt aaagctgtgg gtggaacaat
    11461 aaaaaataac aacggctcca gacgaaaatt tcagattgga acgaccaaat tcagcacaca
    11521 tgagccacat ccaaagaatg tgatggataa aggcgcagta gcaggtctta aggagtggtt
    11581 tgttaactgt gtaggggtag attatgaata aagcatcgaa cattatcaca attaaaggtc
    11641 aaccagccgc agtgacattt gaagccgata tcaacgcatt tcgcggcaag ttcctcaatg
    11701 taaacggtta ttgtgacttt gtagccacaa gtattgaggc attatatcgt gaaggcgaaa
    11761 gcgcgctgga tgagtggatg gcggattgtg aagaggacgg tattgcccct tttcgtgaag
    11821 aagaagagca aaaacgcctg acgcttcgtg ttcctcatca cattgattcg cggctgacca
    11881 tcgtcgccag acaacattcc atttcaaaaa accagctcat tgttgatgtc ttagaacgtg
    11941 agttttcagc ccagatgtaa acaggtcgga tgccgcgtgg catccgacct tttgccagtt
    12001 cgtcaatatt acccatttac aaaatcgtct cttccgtcaa tatccgccgc gcgcctaaaa
    12061 agtgatcctg ccagaaaggt tcagctaacc gacttacccg aatggtttcc ccggtgcgcg
    12121 gtgactcaat aaactgaccg tcgcccagat acacccccat atggtcggca atctcgcggc
    12181 tgtggatatg gaaaaatagc aaatctcccc ggcgcaggtc gttattcgcc acaatggttg
    12241 cccggcgata gtgatacatc tcattagccg tgcgtgggag tttcgcctca aggatcttgt
    12301 tgtaggcata aaaaactaac ccgctacagt cgaagccttg atcgggccgc gtaccgcccc
    12361 agacgtaagg cttgccaagc tgttgctcca gacggtgaat cgccacttcg gtgatgttat
    12421 gcagatggtc gctgcttaag aaatggttgt tttccgccag cgcctgccag cgtctgtcac
    12481 tcgccttgag cggcgcagga aaccattctg gatgctgttt tatctgttcg cggttgtgct
    12541 ggcgtagcgc ccttctgctt tcggcattgc cttccacaat atagctggcc tgttttttca
    12601 gatgggtttg gtactgtttt aataaacgcg cacgattttg catccgctgg cgggcggcgt
    12661 agctgaaagt gatatgtgat gtggtggcgc tggcaaatga agaaaacggc aagcaaagca
    12721 acagtaatcc cgacaacaca aaagagggaa tcgacggcaa ggacatactc aataatggct
    12781 gatcatgcat tgatgttgca gtgtatcccg gcccgtacct gaacaaactt aataaaccat
    12841 tattataaaa ttaataaata tatttcagtt aattatgtaa aaacatccgt ttattaaatt
    12901 actcatggat tgtcgctgtt gctcatttga gctacaattc aagctgaata aatatacagc
    12961 acaggagata ccccaatggc tgctaacgca tttgttcgcg cccgaatcga tgaagatctg
    13021 aagaatcagg cggcggacgt actggccggg atggggctga ccatctctga cctggttcgc
    13081 ataaccctca caaaggtcgc gcgtgaaaag gcattgccgt ttgatttacg cgagcctaat
    13141 caattaacca ttcaatcaat caaaaacagc gaagctggcg ttgatgttca taaggccaaa
    13201 gacgccgatg atttatttga taaattagga atttaaatat gattcaaagg gatattgaat
    13261 actcgggaca attttcaaag gatgtaaaag ttgcacaaaa gcgtcataag gatatgaata
    13321 aattgaaata tcttatgacg cttcttatca ataatacttt accgcttcca gctgtttata
    13381 aagaccaccc gctgcaaggt tcatggaaag gttatcgcga tgctcatgtc gaaccggact
    13441 ggatcctgat ttacaaactt accgataaac ttttacgatt tgagagaact ggaactcacg
    13501 cggcgctctt tgggtaaagc acgctgccga tgaaatttgt aaaccctgcg ccgacaggct
    13561 tgcgtaaaac tcttttggtg ataatgtgag ccttccaaat ccagactatt ccgatttggg
    13621 atctattttg ccgtcctggc gtgtgtaacc gttttatcaa ggaataagca gtatgcgtaa
    13681 aatcgcatta attcttgcga tgttgttgat cccgtgcgtt tcgtttgccg gtttgctggg
    13741 cagcagtagc tcgaccacgc cagtgagcaa agagtataag cagcagttga tgggatcccc
    13801 tgtctacatc cagatcttca aggaagaacg tacgctcgat ctctacgtca aaatgggcga
    13861 gcaatatcag ctgctcgaca gttataaaat ctgtaaatat tccggcggct tagggccaaa
    13921 acagcgtcag ggcgatttca aaagcccgga agggttttat agcgtccagc gtaatcagtt
    13981 aaaaccagac agccgttact acaaagcgat taatattggt ttccccaatg cctatgaccg
    14041 tgcgcatggt tatgaaggga aatacctgat gatccacggc gattgtgttt ccatcggctg
    14101 ctacgcaatg accaatcagg gtattgatga gatcttccag ttcgttactg gtgcgctggt
    14161 gtttggtcag ccgagcgtgc aagtgagtat ttacccgttc cgcatgaccg acgccaatat
    14221 gaagcgccat aaatattcca actttaagga cttctgggag caactgaagc cgggttacga
    14281 ctactttgag caaacccgta agccaccgac cgtttctgtg gtcaatggtc gttacgtggt
    14341 cagcaagccg ttaagccacg aagttgtgca gccacaactg gcatcaaact acacgctccc
    14401 cgaggcaaaa taagcgccat tcgcctggca taatcttttg ccaggtttca ttgcccgtca
    14461 gcggctgtgt tgcaatcacc gtgaccacat cattcggtgt ggtctgcgag ctgaagtcga
    14521 tttccacatc ctgatccagc agcgttgcca cgccaaacgg cgcgcggcgg gtgatccagt
    14581 gtaaattagt cgagcaatac gccatcacat agcgcccgtc agaaagcagc atgttgaaaa
    14641 cgcccttctg ccgcagttca tccgccagtg aggcgatata tttaaacact gccgccatgt
    14701 tgcccggtgt gcgcgggtaa cgctgcgtta atttatgcag gagccagcag aaggcttttt
    14761 cgctatcggt ttcaccgacc gggcggaagt tgccggtttc cagtgatttg tagcccgtca
    14821 gttgtccgtt atgggcataa gtccagttac gcccccataa ctcgcgggta aatgggtgag
    14881 tattttccag cgccacctcg ccccgattag cctggcgaat atgagccacc accgaacagg
    14941 attttatcgg gtagtcctgg acaagtttgg cgatggggga attaaagctg ggttgtggat
    15001 ctttaaatgt gcgacagcct ttaccttcgt aaaaggtaat gccccagcca tctttatgtg
    15061 gcccggttcc accaccacgc tgtacaagtc cggtgaaact aaagcagata tcggtaggga
    15121 cgttggcgct catcccgagc aattcgcaca tgggtaacct ccacaacagc gggagcgcat
    15181 ggcccccggc gaagattgat ttaagaattt tcaggtcgga tgcggcgtaa acgaattatt
    15241 cggcctacgg ttcgggtaca gatttgtagg catgataaga cgcgtcagca tcgcatcagg
    15301 catcagcgca caagtgccgg atgcggcgta aacgccttat ccggcctacg ccagacttac
    15361 ttaaccatct ctttttcaat taactggatc aggatatgga tcactttaat gtgaatctcc
    15421 tgaatgcggt cggcataacc aaagtgcggt acgcgaattt cgatatccgc cgtgccagcc
    15481 attttgccgc cgtctttacc ggtcagggtg atcactttca tccccttctc acgcgccgct
    15541 gcgatcgctt tgatcacgtt tgcagagtta ccggaggtgg agatccccag cagtacatcg
    15601 ccttcgcgac ctaccgcttc aacgtagcgg gagaaaatat cattgaaacc gaaatcatta
    15661 ccgacgcagg aaatatgact aacgtcagaa atagcaatcg ccgggtagcc cggacggttt
    15721 tcacggtagc gaccggtcaa ctcttcggca aagtgcatag cgtcgcaatg ggaaccgccg
    15781 ttgccgcagg aaagcacttt gccaccggct ttaaagctgt ctgctaacag gaccgccgcg
    15841 cgctgaatgg cgtgaatatt ggcgtcatct tttaaaaagt tagccagcgt ttccgccgct
    15901 tcgttcagtt cgttacgaat aagatcctgg tacatgagga tatccttcag cataaatgta
    15961 atagacaaaa tgcagtgtac cggataccgc caaaagcgag aagtacgggc aggtgctatg
    16021 accaggactt tttgacctga agtgcggata aaaacagcaa caatgtgagc tttgttgtaa
    16081 ttatattgta aacacattgc taaatgtttt tacatccact acaaccatat catcacaagt
    16141 ggtcagacct cctacaagta aggggctttt cgttatgatg attttgagta ttctcgctac
    16201 ggttgtcctg ctcggcgcgt tgttctatca ccgcgtgagc ttatttatca gcagtctgat
    16261 tttgctcgcc tggacagccg ccctcggcgt tgctggtctg tggtcggcgt gggtactggt
    16321 gcctctggcc attatcctcg tgccatttaa ctttgcgcct atgcgtaagt cgatgatttc
    16381 cgcgccggta tttcgcggtt tccgtaaggt gatgccgccg atgtcgcgca ctgagaaaga
    16441 agcgattgat gcgggcacca cctggtggga gggcgacttg ttccagggca agccggactg
    16501 gaaaaagctg cataactatc cgcagccgcg cctgaccgcc gaagagcaag cgtttctcgc
    16561 cggcccggta gaagaagcct gccggatggc gaatgatttc cagattactc atgagctggc
    16621 ggatctgccg ccggagttgt gggcgtacct taaagagcat cgcttcttcg cgatgattat
    16681 caaaaaagag tacggcggac tggagttctc agcttatgcc cagtctcgcg tgctgcaaaa
    16741 actctccggc gtgagcggga tcctggctat taccgtcggc gtgccgaact cattaggccc
    16801 gggcgagctg ctgcaacatt acggcactga cgagcagaaa gatcactatc tgccgcgtct
    16861 ggcgcgtggt caggagatcc cttgctttgc actgacaagc ccggaagcgg gttccgatgc
    16921 gggcgcgatt ccggacaccg ggattgtctg catgggcgaa tggcagggcc agcaggtgct
    16981 ggggatgcgt ctgacctgga acaaacgcta tattacgctg gcaccgattg cgactgtgct
    17041 tgggctggcg tttaaactct ccgacccgga aaagctgctg ggtggcgcag aagatttagg
    17101 tattacctgt gcgctgatcc cgaccaccac gccgggcgtg gaaattggtc gtcgccactt
    17161 cccgctgaac gtaccgttcc agaacggacc gacgcgcggt aaagatgtct tcgtaccgat
    17221 cgattacatc atcggggggc cgaaaatggc cgggcaaggc tggcggatgc tggtggagtg
    17281 cctctcggta ggccgcggca tcaccctgcc ttccaactca accggcggcg tgaaatcggt
    17341 agcgctggca acgggcgcgt atgctcacat tcgccgtcag ttcaaaatct ctatcggtaa
    17401 gatggaaggg attgaagagc cgctggcgcg tattgccggt aatgcctacg tgatggatgc
    17461 tgcggcatcg ctgattacct acggcattat gctcggcgaa aaacctgccg tgctgtcggc
    17521 tatcgttaag tatcactgta cccaccgcgg gcagcagtcg attattgatg cgatggatat
    17581 cactggtggc aaaggcatta tgctcgggca aagcaacttc ctggcgcgtg cttaccaggg
    17641 cgcaccgatt gccatcaccg ttgaaggggc taacattctg acccgcagca tgatgatctt
    17701 cggacaagga gcgattcgtt gccatccgta cgtgctggaa gagatggaag cggcgaagaa
    17761 caatgacgtc aacgcgttcg ataaactgtt gttcaaacat atcggtcacg tcggtagcaa
    17821 caaagttcgc agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg
    17881 cgatgccact aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct
    17941 ttctgatgtc tcgatggcag tgctgggcgg cagcctgaaa cgtcgcgagc gcatctcggc
    18001 ccgtctgggg gatattttaa gccagctcta cctcgcctct gccgtgctga agcgttatga
    18061 cgacgaaggc cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac aagatgcgct
    18121 gtatcaggct gaacaggcga tggatgattt actgcaaaac ttcccgaacc gcgtggttgc
    18181 cgggctgctg aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa
    18241 gctggatcat aaagtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg
    18301 tcgcggtcag tacctgacgc cgagcgagca taatccggtt ggcttgctgg aagaggcgct
    18361 ggtggatgtg attgccgccg acccaattca tcagcggatc tgtaaagagc tgggtaaaaa
    18421 cctgccgttt acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga
    18481 taaagatgaa gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt
    18541 tgatgacttt gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg
    18601 gaaagttgaa gccgcgtaaa tgaataacgg agccgaaagg ctccgtttct ttatccgcta
    18661 attatttaaa attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca
    18721 acaccgtttt accatcaatc gaaagcgcat cgttcacatt cagccaggtg agtttctctt
    18781 gcgacgtttt ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc
    18841 acatcacagc gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat
    18901 acgtctcatc tcccaacgtt acggtttgtg cgggagtata agtaccgcct ttcatcaccc
    18961 aggcaggcag cttatgccct tgtaccatct gattaaatgc agctttggtg gtttcgccct
    19021 ttgcaaggct gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag
    19081 cggcgactgt cgttattgcc ttaaacatca ttcctcccga gcttatcctg cccatgttat
    19141 taaccttcta aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag
    19201 catttgttat caatttattc tgcgctaagg caactgcctg ataaggtgtt aaagttataa
    19261 aacccttcgc ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca
    19321 accactggtg tggatggatg gccctgccaa cctgcgtcat tttgatcgtc aactggaagg
    19381 tattaccggg cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga
    19441 agcggcagct tcgtcgctag cacaagatga cgtagtgaac tggatgacag ctaaggcgca
    19501 gcagtgcaat gcgctgattg caggcagtgt tgcattacaa acggagtctg gttcggttaa
    19561 ccgctttttg ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt
    19621 ccgcatggca gatgagcatc tacattataa agcgggcaat gcgcgagtga ttgtggaatg
    19681 gcgcggctgg cgtattttgc cgttggtgtg ctacgactta cgttttccgg tgtggtcgcg
    19741 caatctcaac gattatgacc tcgccatcta cgttgccaac tggcctgctc cgcgctctct
    19801 gcactggcag gcattgctga cggcccgcgc gattgagaat caggcgtatg tggcgggatg
    19861 caatcgcgtc ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa
    19921 cccgcagggc gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga
    19981 gctgtcgatg gtggcattgc gggaatatag agaaaagttt ccggcatggc aggatgcgga
    20041 tgagtttagg ttgcggtgag tgggggttgt gtttaaggct gggggagagt cggggtaaga
    20101 ctacgaaagc ccgctctccg caaggactga cgccagatag tttctgtcca tagctgcttt
    20161 tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa tattaatagc
    20221 gatgtgccgt atccctgaaa ataattctgc ggcgtttcct cttcttattt tgcagtcgtc
    20281 ttcattcatt accacgtcca gacgccagtg cagcttattc tccacgtgcc agtggtttcg
    20341 gattgctgtg gcgaactttt ctgcggttaa atcagcagaa ctgatataat atctgaccgt
    20401 catttctggc tctttctttt gttctgctat tattgaccga aaggagactg ccacgcataa
    20461 tttcttcagt cctttccatt caaacgtgaa atcaataagt tcatcaggga catcgcaaac
    20521 aatatgaaga cggatttctt ctctgccgtg actcttttca ctcattgcgt aactgtcatg
    20581 ctctggatta tttaattctt tcagcggaaa tttttcctca aaggctttat ttagccgccc
    20641 c
//
LOCUS       DAZFFI010000079        20264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFFI010000079 DAZFFI010000000
VERSION     DAZFFI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.079"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(197..943)
                     /gene="cutC"
                     /locus_tag="ACHJ12_003400"
     CDS             complement(197..943)
                     /gene="cutC"
                     /locus_tag="ACHJ12_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4991137.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(957..1523)
                     /gene="yecM"
                     /locus_tag="ACHJ12_003401"
     CDS             complement(957..1523)
                     /gene="yecM"
                     /locus_tag="ACHJ12_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4991138.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1739..3472
                     /gene="argS"
                     /locus_tag="ACHJ12_003402"
     CDS             1739..3472
                     /gene="argS"
                     /locus_tag="ACHJ12_003402"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4991139.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPRIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3514..3558)
                     /gene="yecV"
                     /locus_tag="ACHJ12_003403"
     CDS             complement(3514..3558)
                     /gene="yecV"
                     /locus_tag="ACHJ12_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO4991140.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3649..4137
                     /gene="yecT"
                     /locus_tag="ACHJ12_003404"
     CDS             3649..4137
                     /gene="yecT"
                     /locus_tag="ACHJ12_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4991141.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4257..4649)
                     /gene="flhe"
                     /locus_tag="ACHJ12_003405"
     CDS             complement(4257..4649)
                     /gene="flhe"
                     /locus_tag="ACHJ12_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4991142.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4649..6727)
                     /gene="flhA"
                     /locus_tag="ACHJ12_003406"
     CDS             complement(4649..6727)
                     /gene="flhA"
                     /locus_tag="ACHJ12_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4991143.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6720..7868)
                     /gene="flhB"
                     /locus_tag="ACHJ12_003407"
     CDS             complement(6720..7868)
                     /gene="flhB"
                     /locus_tag="ACHJ12_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4991144.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8070..8714)
                     /gene="cheZ"
                     /locus_tag="ACHJ12_003408"
     CDS             complement(8070..8714)
                     /gene="cheZ"
                     /locus_tag="ACHJ12_003408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4991145.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8725..9114)
                     /gene="cheY"
                     /locus_tag="ACHJ12_003409"
     CDS             complement(8725..9114)
                     /gene="cheY"
                     /locus_tag="ACHJ12_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4991146.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9129..10178)
                     /gene="cheB"
                     /locus_tag="ACHJ12_003410"
     CDS             complement(9129..10178)
                     /gene="cheB"
                     /locus_tag="ACHJ12_003410"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4991147.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10181..11041)
                     /gene="cheR"
                     /locus_tag="ACHJ12_003411"
     CDS             complement(10181..11041)
                     /gene="cheR"
                     /locus_tag="ACHJ12_003411"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4991148.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11060..12661)
                     /gene="tap"
                     /locus_tag="ACHJ12_003412"
     CDS             complement(11060..12661)
                     /gene="tap"
                     /locus_tag="ACHJ12_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4991149.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12707..14368)
                     /gene="tar"
                     /locus_tag="ACHJ12_003413"
     CDS             complement(12707..14368)
                     /gene="tar"
                     /locus_tag="ACHJ12_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4991150.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAIIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14513..15016)
                     /gene="cheW"
                     /locus_tag="ACHJ12_003414"
     CDS             complement(14513..15016)
                     /gene="cheW"
                     /locus_tag="ACHJ12_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4991151.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15037..17001)
                     /gene="cheA"
                     /locus_tag="ACHJ12_003415"
     CDS             complement(15037..17001)
                     /gene="cheA"
                     /locus_tag="ACHJ12_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4991152.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17006..17932)
                     /gene="motB"
                     /locus_tag="ACHJ12_003416"
     CDS             complement(17006..17932)
                     /gene="motB"
                     /locus_tag="ACHJ12_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4991153.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17929..18816)
                     /gene="motA"
                     /locus_tag="ACHJ12_003417"
     CDS             complement(17929..18816)
                     /gene="motA"
                     /locus_tag="ACHJ12_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4991154.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18943..19521)
                     /gene="flhC"
                     /locus_tag="ACHJ12_003418"
     CDS             complement(18943..19521)
                     /gene="flhC"
                     /locus_tag="ACHJ12_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4991155.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19524..19874)
                     /gene="flhD"
                     /locus_tag="ACHJ12_003419"
     CDS             complement(19524..19874)
                     /gene="flhD"
                     /locus_tag="ACHJ12_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4991156.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4863 a         5573 c         4930 g         4898 t
ORIGIN      
        1 caatttaaat cccataatca atgtgaatat aaaaaatatt attagcacaa cgctatgttc
       61 actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa
      121 aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat
      181 gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa
      241 ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg
      301 acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga
      361 cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccgg
      421 ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca
      481 gtgcgtctga tttttgccct gatgtcagca cccgggcaat gcccaattcc gcaagattat
      541 tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg
      601 gaccggcagc agccattatt ttttccattc gtggcatatc gacattcccg tcaacatcga
      661 gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa
      721 tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat
      781 gcacagggat cgtcacccgc tggcgcacgg atttcagtac ccccaacgac ggcgttaagc
      841 ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta
      901 gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac
      961 gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt
     1021 ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa
     1081 ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca
     1141 cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg
     1201 tgtgggtaac gtttttcccc gggccacggt agctccacaa tagaaaactg ccagtgcgca
     1261 acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat catattttct
     1321 gacaaaagct cgccacactg ttcaaacccg cgacgccagc gttcagcagt ggcgttttga
     1381 tggcaacgca aagaaatgtg atcggcagtc agcggagtga tattcaaccc cagacggagg
     1441 gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg
     1501 tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac
     1561 cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa
     1621 cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccgccc
     1681 taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt
     1741 gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc
     1801 tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc
     1861 taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca
     1921 ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg
     1981 ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc
     2041 gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc
     2101 gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc
     2161 agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga
     2221 ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc
     2281 cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga
     2341 cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga
     2401 cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat
     2461 cacctacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta
     2521 caacccgatg ctgccaagaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag
     2581 cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg
     2641 cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc
     2701 gaaatatcgt tatgaaacac tgcatgccga tcgcgtgcta tattacatcg actcccgtca
     2761 gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc
     2821 cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa
     2881 aacccgcgcg ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc
     2941 acgccgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc
     3001 taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacaga
     3061 ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca
     3121 gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact
     3181 ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct
     3241 gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc
     3301 ttacctgtac gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag
     3361 cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac
     3421 gctaaagctg ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt
     3481 tcgtgagagt aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg
     3541 tattctgaaa atggacatgc cattgttttc tgactgttgg ataagaggcc agaagcgtaa
     3601 tatccggccc cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt
     3661 cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta
     3721 aacgaccatg acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact
     3781 gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta
     3841 aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa
     3901 aaacctgata ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt
     3961 agggacaaag aatgtgaatt aatcctttca aatgaggacg ttcaggattt aagtgaccct
     4021 tattctgaat cagaatggct ctcatgtatg atcatacaga ccaatacgcg aactcgccag
     4081 ttgcagctat accataactc tgaagatttt tatccaagcc ctttgacaag aggataattc
     4141 acatcttttt ggcatgtttt gttgcaagct attcctgata aataattgca acaagacatc
     4201 gagccttttt cactgagtta ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag
     4261 cgataattca caatcacttc attacgttgt acctttagcg gtggaattaa ccgcccaccg
     4321 cctggcactt cccagataaa tcgcaacggt tctgctgccg ctataccgga aaaggccacg
     4381 gtggttccgc tctgcccctc taattcgaca caacgagatt gcgaacacaa gcgaacccgc
     4441 agtcctgaag gtgtcgggcc gataagctga taacgccacg ctaccagcgt catcaatcct
     4501 gaagcaggtt gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg atgatttagc
     4561 gtaattccta cactacttgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga
     4621 aacaataata ttgctaataa ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga
     4681 tatgtcggtt atcagacagt tccagattcg acaggaccac taactgcggc aagctgcggc
     4741 gcaggaagcg agacaataat ggtcgcagcg cgtggttcac caacaatact ggcggcgcac
     4801 ccagcatctc ctgacgggat agcgcttcct gagtttgcgc cagtaaacga tccgccagcc
     4861 ctggctccag tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat
     4921 cgaggccaat aacatggact tcatctttgc caggaaacca ctgctgggta atcgcccgtc
     4981 ccaacgccac gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt
     5041 ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca tcgaggagat
     5101 tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca
     5161 gctttggcat ctcctgggcg acgcgatcca acagctgttg cgcctcctga cgaccaaaca
     5221 gctctgcggc atgctggcta atgaggtggt taagatgcgt tgctaccacc gtgctggcct
     5281 caaccactgt gtacccctga atctgcgcct gttcttttag cgcactttca atccagatag
     5341 cattcaggcc aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggttc
     5401 cagggttaat cgccagccag cgccccggat aagcatcacc actgccaatc tccacgcctt
     5461 tcatcaaaat gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg
     5521 gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat actgcgtata cggcccaaca
     5581 actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg ataacccact tccattccca
     5641 gagaatcttc cagttgtaca tcgttccacg tcgcttcgac aacggtatta ttctctgcca
     5701 tttttaccgg tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc caccaggcca
     5761 gccccagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc
     5821 cgagtaaacc gagcacggcg gcgcttaaca acataacgct tgggttactg aaaagctgat
     5881 tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg
     5941 cggtagaaat caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg
     6001 tataactttc cgccgcgtgt cccatgctca tgccatgttg cagcacgccg accagcaacc
     6061 cgccgacaac gttaatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact
     6121 tacttgcccc gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt
     6181 ttttcgcctc atcttcacca atcaatccag cgttaaggtc ggcgtcaatc gccatctgct
     6241 tacccggcat accatcgaga acaaagcgcg cacccacttc tgcgatacgc ccggcacctt
     6301 tggtaatgac cataaagttg atgatcacga gaatgacaaa caccacgata cctatagcga
     6361 aattgccacc aacgaggaag tgaccgaacg cttcgaccac cttccctgcc gccgccgcgc
     6421 cggtatgccc ttccatcaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca
     6481 acgtagtaaa caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca
     6541 tcgccaccag caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta
     6601 tgaatgcggg cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg
     6661 caaggatctg ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat
     6721 tactcatggg tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta
     6781 ggttgtacag ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc
     6841 agcacttccg ccaccgcggc gtacagttga cccgggattt gttgaccaat ctccgcatgt
     6901 cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca
     6961 atttcacgaa tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc
     7021 attttgtttt cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca
     7081 tccgctttcg gcacatcggc catcatccga cgccgtgcag cagctcgctg catctgacgg
     7141 atccgccctt taacatgagg gtcaccttcg ctttgtttga actcatcacg aatatcctgc
     7201 cgtgacatac gcagcttttt caggtggctg aatatttgga aaaagacgtc aaatcccacc
     7261 attggaatga caccaagcac caccagcagt gcgcatagcc ctaccaaatc catcgcatta
     7321 cccatggcgg taatcggaga ctcggccatc aagcgcatca tctgcggcca gtgatgccag
     7381 agaaaaaacc ccgtcacgct gccaaccagg atggttttca aaattgcttt aagcaactcc
     7441 gcgccagtct gagccgagaa catccgttta atgcccggta gcgggttgag tttggaaaac
     7501 ttcggctgca aggatttgcc gctaaatacc agccctccca gcatgaccgg agaaatgagc
     7561 gccaccagca ccacgccgct aatcagcggc agcagcgcca gcatggcttc tctgatcagc
     7621 agaataatct gcccgaggat cagattcggg tcattgataa tactgtgatc aaaatgcagc
     7681 ccagcggaga gcatgcccga caatcgacgg gccagcgaca caccgccaaa ccagataaca
     7741 ctaacgccca ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg gatttgcccc
     7801 tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg
     7861 tcagacacgt cgccaatcct ggaaagagta ttaagcgtga atgatgccag agcgcaaagc
     7921 gttcaatggt ttgagtaagg ggcaaaacag gcgggattta gggcttttgc tgccacacat
     7981 caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg cctgatatga
     8041 cgtggtcacg ccacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc
     8101 acctgatcct gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc
     8161 aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc
     8221 atcagcaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat gacctgcccg
     8281 gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg
     8341 gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg ttaccagttc acgggcgtcg
     8401 gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat
     8461 ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca
     8521 gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatggcttcc
     8581 gccgcttcgg caatggcctg atccagcccc agttcccgca aactgtcgcg cagcatacgc
     8641 gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt
     8701 gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt
     8761 ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc ccgcttgcgc
     8821 cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgccga
     8881 catcgcgcca tccgcacgaa ttgttttcag caattccagg ccatccatat tgggcatgtt
     8941 ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgacgcc
     9001 atcttccgct tcctcaacat tattgaatcc cagctctttc agcaggttac gcactatgcg
     9061 tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca
     9121 ctcctgattt aaatacgtat cgcctgtccg gcactaattt ttgccaacat ttgctggctt
     9181 acctggctaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg
     9241 ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc
     9301 gccaacattc ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc aaccgcatta
     9361 cgccccgcct gtttggcgac agaatggaac aacacatcta ccgaaggccg atgacggtta
     9421 accgccgggc catcgtgaat tttgatttgg taatttgcgc cactacgcga cagctccata
     9481 tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga cacgttctcc gtcttcggct
     9541 tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg ggtgaaaccg
     9601 ggcggcatat gttgggtaat taacaatgcc gggctggaaa gcggcaacgg ttgcagtacg
     9661 tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc
     9721 aacaacggac ccgccttcag cgttgtcggt gctgacaatg gcttatgtgc tgcaaggctc
     9781 gcctttgctg ctgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg
     9841 cgaataccca gttgcggttt ggtgacaaaa tctatcgccc ccagctccag cgcgcgcagc
     9901 gtgacttctg accctttgcc agtcagggaa gaaaccatca ctacgggcat tggacgcaaa
     9961 cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc
    10021 agcacatcgg gattgaattt cttaatcaag tcacgcgcga ccagcggatc aggcgcggtc
    10081 gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt
    10141 gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat
    10201 acaccgtctg accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac
    10261 ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct
    10321 gggtattttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg
    10381 gcacggtgta ctgtttcgcc agtagattca gcggggcaaa atcaacatag ttcgccagct
    10441 cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca
    10501 gttgctgcgg cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt
    10561 ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc gcggtgccca
    10621 atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa gccgccgcgc
    10681 tccatacgcg atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga gggaaatggt
    10741 gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact
    10801 caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc gtcagtccca
    10861 gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcagccagaa
    10921 cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg
    10981 ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca
    11041 tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc gccgactcat
    11101 gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg
    11161 cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag gcgttctgct
    11221 gagtcacctg atccatctgg ctgacagcct gtgcaacctg ctcaatcccc cgctgttgtt
    11281 cttccgacgc agaggcaatt tctcccataa tgtcgttcac gcgggtcacc gaactgacaa
    11341 tatcgatcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg acacgattga
    11401 ctgactcttc gatcagccct ttgatctctt ttgctgcctg cgcgctacgg ctggcaagat
    11461 tgcgcacttc acctgccact accgcaaaac cacgcccctg ctctccggcg cgagccgctt
    11521 ccactgccgc attcagggcc agaatattgg tctggaaagc aattccgtcg ataacgctga
    11581 taatgtcgcc aattttttgc gagctggtgg cgatctcctg catggtgtga gtcatggtac
    11641 tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt tccgacgcct
    11701 gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata ctggcggccg
    11761 tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcgtta ttgcctgcga
    11821 caatctccgc gataccaatg tgcatctcct ggcttccctt acgcacatca cttaccgtcc
    11881 cacgcaaagc ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc tcattacgac
    11941 catataccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa tggctaccga
    12001 taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg ctgatgtaga
    12061 ttgccgcaac aataatcatg ctgataaaca ccagtgccga aatctgatag ttacgctggc
    12121 tttgcgcact ggcggcttcc agcacatggt tgatctccag ctgccaggcc tcaaagttaa
    12181 cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa agttgattac
    12241 tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg
    12301 tctctttttg caatgccctg acgtgctcgt tgcccgcagt catcgccata aaacttttaa
    12361 acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat
    12421 ccgccggata gctaagtgcc gtcagagtgc ccgctttgtt cagcgcggta ctggcctgta
    12481 acattaccgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat
    12541 tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca
    12601 agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca
    12661 tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttccc
    12721 agtttggatc ttgttcagca attcgcagtc gtggctgagc cggtggttgc tcactggcag
    12781 gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacgg aacgcggaaa
    12841 ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc ggcggcggca gctgattcct
    12901 gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt
    12961 gatcgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca
    13021 cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg
    13081 aaccggtatc aacgcgtgag acggagtctt caatgagggc tttgatctct tttgccgcct
    13141 gggcgctgcg actggcaaga ttacgcactt cacccgccac cacggcaaaa ccacggccct
    13201 gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc gaggatatta gtctggaagg
    13261 caataccgtc gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat
    13321 gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg tcggaggcac
    13381 tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga
    13441 gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttca gtacgggagg
    13501 aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt
    13561 ctcgcacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca
    13621 ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta ccaccggcga
    13681 tttcgcgaat gtgagcaata atttttgcca gcggagtaag caacatacgg cgaatgccgt
    13741 accacgccac cagcagaatc aataccacca ccagcgcgat aatcgccagt tgccactgcg
    13801 caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt tcactgctga
    13861 gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt ggctgagcga
    13921 aataagctcc agtattgcca taatcaagat aatcaatcag ttcagttaac gctgtgtgat
    13981 agtttttata tttttcatca atattacgac tggtggcgac catttcaggt aacggtgcca
    14041 tgcttttgaa ttttttataa tgcgtcgcgg cctgggccaa tgttttcctg gcgctatcga
    14101 gcaattcaac tttggcatta ctttgttgat tggaggaatc catcatcatc cgcaccgctg
    14161 aacgactcag gttaatacgc gtttgcagca ttaaatccca ggttgacgtc agctcgccct
    14221 gctgttcccg taattgattg gaaaccacaa agctcttctg gctatggtga agggaagaaa
    14281 aaaacagact gccggaaata agttgtaaca gtgcgaatac ccccagcacc attaccaaca
    14341 gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag ttgatctcgt
    14401 tatcggcaag aaggggggaa actttattgc tgatgccacc cgccgcgaaa ttgaaataaa
    14461 aaacccgatg cgcagatcat cgggttcatt tcaattgagg aaatcgggag aattacgcca
    14521 cttctgacgc cgcgctatct aacagcgcca tctcttcgct attcagcagt ttttcgatgt
    14581 tcaccagaat caacatacgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa
    14641 gcgtcacggc aaattccggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg
    14701 aaacgccgtc aactacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg
    14761 tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca
    14821 caataacccc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg
    14881 ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat
    14941 caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat
    15001 tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg
    15061 ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc
    15121 gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt
    15181 aaccacaacc tggtgctgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc
    15241 actttgtaag atcaccacaa ttccctgggt ggcttcggtt ttcgcgcccg cgacgttgaa
    15301 cactttccac agttcgacga tgggcagata ttcgccccgc acttccagca cccgctcgcc
    15361 gccggccagt ggatgcagat cggcttcacg gggttgcagt gattccatga cagcattcag
    15421 cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt
    15481 cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcgacatg
    15541 accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac
    15601 gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc
    15661 gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc
    15721 acggtttagc cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc
    15781 ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag
    15841 ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc
    15901 tatcaggctc ttgtcgagct cggtggaact gcccaccagc gtcagttcta cctgcttgcc
    15961 gagttttccc gccagatcac gcaccagccg gggatagcga ctaaagacat attccatcgg
    16021 catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc
    16081 catgctggta atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag
    16141 catggactgg gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac
    16201 cgctacacgg atgctggtgg attcactgct gcgcgtcgtt ttttcccgct ccacgcgacc
    16261 ggttggcgct tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac
    16321 ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat
    16381 gtcatcttcg gcgatatcgc ccggtaatat tgccgagaga gaatccgccc ctttcaccac
    16441 gtcagttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt cgccggcctt
    16501 caggcgcgaa aggataattc gtcgcggcga ctgactgcga ctctgctcat cttgcggttc
    16561 acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa
    16621 tgccagttga cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga
    16681 ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat
    16741 aatgtcggtg ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag
    16801 atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg
    16861 ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag
    16921 cagatgctgc tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa
    16981 atcgcttata tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac
    17041 tgacctgtgg tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg
    17101 cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac
    17161 ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacga
    17221 cacgtaacac tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg
    17281 cccgatcggc agaaagctcc cagttgctat atcctttctc accgctggcg taggggaaat
    17341 catcggtatg acctgaaagg ctaatacggt tgggaatacc gttcagtaca ggcgcaatgg
    17401 cacgcagaat gtcgcgcata tagggttcga catcggcact gccggttcta aacatcgggc
    17461 gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat
    17521 ggggacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga tcaccccgca
    17581 atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc ggctgcttat
    17641 tcacttcccc ctggctttgg gtgtaatcat caccaccgcc gggaattggg ctttcactat
    17701 tagaaatgcg atcgccgccc gtaaccgcag tcgccagtgg agtccggaag tactccgcaa
    17761 tctgaatcag ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca
    17821 tcatcgcagt cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc
    17881 ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc
    17941 ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa
    18001 cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc
    18061 gtaaccgttc agattagaaa gcagagtgac tttgacgcac tgcatcattt tgctggtttc
    18121 ggcgcttttc tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat
    18181 gccgaggaaa gtccccacca tcgcatgtgc gataagcgca cccagctcgg cggcaggacg
    18241 atcggctgaa cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg
    18301 aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt
    18361 ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatatgac cgctgataat
    18421 cagacgcaga taatcgacga taaaatcaag catgacgcta tccgcgagga tgcgtgggta
    18481 gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc
    18541 catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc
    18601 tttggtgtat ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc ctttaatcgc
    18661 tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa tcaccagttc
    18721 agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt
    18781 accgagaaca accaggtaac ctaataagat aagcacgaca ttatccttcc actgttgacc
    18841 atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt
    18901 acgtcgttac cgctgctgga atgttgcgcc tcaccgtatc agttaaacag cctgtactct
    18961 ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt
    19021 tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc
    19081 gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc
    19141 aacaaaccgc accaatgtcc aggcacgggt taatgccagc agtggtcctt cttctgcttg
    19201 tgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa
    19261 accggttttc agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc
    19321 ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg
    19381 cagttcttta taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat
    19441 ctgcaaacga gctcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg
    19501 aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga
    19561 ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg
    19621 tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa acggaagtga
    19681 caaaccagtt gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaac
    19741 gttgtcgcca tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca
    19801 atcaaacgct gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac
    19861 tcggaggtat gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg
    19921 caccccgtga tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga
    19981 taagctgcaa taagcagaac cacctttttg gtttaatatg tccttacaaa tagaaatggg
    20041 tctttacact tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca
    20101 catacaacgg aggggggctg cgattttcaa taatgtgtga tgcagatcac acaaaacact
    20161 caattactta acataaatgt ataagtcata cttttgtttt gggtgtattt caatctgtta
    20221 aaaagttttt cgctacgcta gcaagcaaaa atgaaacagg aata
//
LOCUS       DAZFFI010000080        20023 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFFI010000080 DAZFFI010000000
VERSION     DAZFFI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.080"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2050
                     /locus_tag="ACHJ12_003420"
     CDS             <1..2050
                     /locus_tag="ACHJ12_003420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFO4991157.1"
                     /translation="RKSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNAL
                     DAYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARK
                     EWNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSK
                     NNLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRL
                     NNDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFR
                     KIRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYK
                     IGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATG
                     SAGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILM
                     GDGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMD
                     KGHAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKI
                     PHNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFT
                     EDEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ
                     "
     gene            2083..3234
                     /locus_tag="ACHJ12_003421"
     CDS             2083..3234
                     /locus_tag="ACHJ12_003421"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4991158.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQENAYFTGDKIKIFSLRHGELNSRIA
                     VFLISAMKKSFSTFSWGSSSFNENVLNKVKINIPTFNNVPAYNYMVKYIEELEAAHIE
                     ELEAAHIEELEAYLIAAGLSDYQLTEKDYLILEYFKNIEFSGFPVTELFSVCNTGNIL
                     SRDIIENSGDVPYLCASRENNSVSSYIAYDDSLLEKGNCIFIGGKTFVVTYQERDFFS
                     NDSHNLRLYVNNEDGRTKFAYLGIISCIYRSISHKYSWGDSVSNRKIKNDVIWLPVKN
                     KSPDFCYINDLISVVHKLVIKDVVQYAERKMNAYRQAITSRGYDDSGVS"
     gene            4013..5926
                     /locus_tag="ACHJ12_003422"
     CDS             4013..5926
                     /locus_tag="ACHJ12_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4991159.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSDRDISSPAEPGSHPTVSTKPAAEPEYYYVIALAGQSNGMSYGEGLPLPDT
                     FDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVEDMTGLNHPKADSSKGQYG
                     CVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDTTGASESSTRWGVD
                     KPLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFSGTPANHTAQFGALVDKYRADLA
                     DMVGQCVGGSADGVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GANVPTNKPEEDPDIPGIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAI
                     LSCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLV
                     DDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNK
                     YAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTGNQQKELGTFGAFDHDWH
                     TLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANELNRFVITDITGSAETYPVLID
                     TVEVKANKAGAAA"
     gene            6069..6251
                     /locus_tag="ACHJ12_003423"
     CDS             6069..6251
                     /locus_tag="ACHJ12_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4991160.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGTKPEEPLIP"
     gene            6277..6549
                     /locus_tag="ACHJ12_003424"
     CDS             6277..6549
                     /locus_tag="ACHJ12_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4991161.1"
                     /translation="MSEIKPLVTAEAVKDVIRSEEVRSALKQKLRQNLEERLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAENGKPQPELPVADATEPQPETVMML"
     gene            6626..6841
                     /locus_tag="ACHJ12_003425"
     CDS             6626..6841
                     /locus_tag="ACHJ12_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4991162.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6846..7379
                     /locus_tag="ACHJ12_003426"
     CDS             6846..7379
                     /locus_tag="ACHJ12_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4991163.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVLPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGQDCRIRSNNCYGQ
                     VSRRDQESALACWEIDR"
     gene            7600..7713
                     /locus_tag="ACHJ12_003427"
     CDS             7600..7713
                     /locus_tag="ACHJ12_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991164.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            7715..8182
                     /locus_tag="ACHJ12_003428"
     CDS             7715..8182
                     /locus_tag="ACHJ12_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4991165.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTAERDYFNLRERLMTMQKQLEGAQEYIHTQCLN"
     gene            8324..8554
                     /locus_tag="ACHJ12_003429"
     CDS             8324..8554
                     /locus_tag="ACHJ12_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HFO4991166.1"
                     /translation="MATSIRLDDDFVSDVKVYADAASRSIPKQIEYWAKIGRIAEDNP
                     DLPYSFISEVLLSQSEIRHNKVTRYVRKTDRS"
     gene            8529..8858
                     /locus_tag="ACHJ12_003430"
     CDS             8529..8858
                     /locus_tag="ACHJ12_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4991167.1"
                     /translation="MSEKQTGREIDVYQSRRFEKSLTKLPEAQLKVVEDEIDRIIDNP
                     LLGTQKKGDLSFLRVHKFQLNNQQVLLGYSWIDDKLELYLLSIGPHENFYTDQKKHRK
                     ADLKLIG"
     gene            8988..9604
                     /locus_tag="ACHJ12_003431"
                     /pseudo
     CDS             8988..9604
                     /locus_tag="ACHJ12_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            9897..10703
                     /locus_tag="ACHJ12_003432"
     CDS             9897..10703
                     /locus_tag="ACHJ12_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4991168.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRNSRSGNP
                     HPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            10684..12390
                     /locus_tag="ACHJ12_003433"
     CDS             10684..12390
                     /locus_tag="ACHJ12_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4991169.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            12390..14534
                     /locus_tag="ACHJ12_003434"
     CDS             12390..14534
                     /locus_tag="ACHJ12_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO4991170.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QESGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGIQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            14692..15699
                     /locus_tag="ACHJ12_003435"
     CDS             14692..15699
                     /locus_tag="ACHJ12_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991171.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EETQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            15723..16937
                     /locus_tag="ACHJ12_003436"
     CDS             15723..16937
                     /locus_tag="ACHJ12_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO4991172.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            16993..17382
                     /locus_tag="ACHJ12_003437"
     CDS             16993..17382
                     /locus_tag="ACHJ12_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991173.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSVKSGEHVLIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLSVLLRYTVVGY"
     gene            17435..17896
                     /locus_tag="ACHJ12_003438"
     CDS             17435..17896
                     /locus_tag="ACHJ12_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991174.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERSLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            17880..18443
                     /locus_tag="ACHJ12_003439"
     CDS             17880..18443
                     /locus_tag="ACHJ12_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991175.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            18443..19093
                     /locus_tag="ACHJ12_003440"
     CDS             18443..19093
                     /locus_tag="ACHJ12_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO4991176.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            19090..>20023
                     /locus_tag="ACHJ12_003441"
     CDS             19090..>20023
                     /locus_tag="ACHJ12_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO4991177.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMEPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKDSAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDIGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT         5422 a         4401 c         5466 g         4734 t
ORIGIN      
        1 ccgtaaatca agaaagtctg taaaatctgt tgaaccagac attgccgacc gggctaacag
       61 ctggctcaaa tcttatggac ttgattacaa actggagcag gcaccactta acaacgagat
      121 cgataacgca ctggatgctt atttttcgaa aaatggcggc gcaggcggta atcgtcccga
      181 cgtaaaactc cttgttcagg acaaatacgg caagcagtat cccgttctca tcgagtataa
      241 aggctacaag gacaggctta tcagacttgg cagcaacggt attattgaga acaaagatgc
      301 cagaaaagaa tggaatttta aaaatattaa tggatacgcg gtgaatggcg cagtccatta
      361 tgccaacgca cttcttcagt tcaccaatta ccctgatatt attgctattg gtatgaccgg
      421 ctggcgcgat gatgatacag gcgaactgca tcatgaaatc ggcgtgtggt atgtatcaaa
      481 gaataattta ggagccgggc agaaggttgg tgaattcacc gacttatcat tccttgctga
      541 caaaaatgtt gatggatttc tcaataagat aaagctgctt aatcttcctc ccgaagaact
      601 ggaaaaaatc aaggccagca aagaggaaga gatagacacg cgactttccc gtctgaataa
      661 cgatatttac cagaatgaaa aggggcttgg tgaaagcgat cgtgtttacc ttgttgtggc
      721 taccgttatt gcaacgttgg ggattccggg aaagttagca ccgctggata aaaaagaact
      781 gacctcttca acggaggagg acttgcgtga tggtgatatt attttcagga aaataaggaa
      841 ttttttaagg ctgaaagctg ttcctgaaac aaaaagagaa atgattttgc gctcgttgca
      901 aaatacgctg tggactgaaa acattaacaa accagtcaac ggtgaaagcc agttaaagcg
      961 cgtgtttgtt aaagttgttg atgatcttgg ggagtattac aaaatcggac tgaccaccga
     1021 ttttacgggc aaactgttta atgagatgta tcgctggctt ggatttacgc aggacaaact
     1081 caatgacgtc gttctgacac caccttatgt tgccacattg ctggcacgac tcgccagagt
     1141 aaacaaagat tcctatgtgt gggattttgc tacaggttct gcgggattgc ttgtggctgc
     1201 aatgaatgaa atgctcattg acgccagaga aaatattcac tcaccgaatg aactacaact
     1261 taaggaagcg caaatcaagg ctgaacagct tcttggcctt gaggtgttat ccagtattta
     1321 tatgctggcc atcctgaata tgattttgat gggggatggt agctcgaata tcctcaataa
     1381 agactcgctg gccgattttg acggcaagta tggatttggg aaaacaggag aaaaattccc
     1441 tgcggacgca tttattctca atcctccgta ttcagctaaa ggtaacggca tgattttcgt
     1501 gcagaaggcg ctgtcgatga tggataaagg ccatgctgca gtgattatcc agagttcagc
     1561 cggcacagga aaggctacgg agtacaacaa aaaaatactc aaggaaaata ccttgctggc
     1621 aagcatcaaa atgcctgcgg atctatttat tggtaaatca agcgttcaga cgtacattta
     1681 cgtttttcag gtaaaaattc cgcataacgc gaaacaggct gttaagttta tcgacttttc
     1741 caatgacggc tatgctcgct ctaatcgcaa aaaggcaaga aacaatcttg ttgatgccga
     1801 tcgtgcaaaa gagcgttatc aggaggttgt ggatctggtt cacttcggga aaggttgcct
     1861 taatatcttt acggaggatg aatattttga gggaacaatc gatcctgata gtggtgagga
     1921 ctggaatcag accaggccag tagataccag accgacactg gaggatttta aaaagacggt
     1981 tggcgactat cttgcgtggg aggtgtcaaa acttcttcag gggcgtgatg cgtcagaaaa
     2041 aaagcagtaa actctcgtct tgcagagctt gagcgggagt ttattaaatc tggtggtaaa
     2101 tgggaagaat acacaatcga aagattgttc agtattcaga cacctaaaaa gaaatttaat
     2161 gccaataaat taacatttaa tggcaactat ccttatgttg ccagaggaag taacaataac
     2221 ggcataaaag gatatataga tgaagatgaa ttatatctca acccggggaa tacaattagc
     2281 tttggtcagg atactgcgac catgttctat caggaaaacg catatttcac aggtgataaa
     2341 ataaagattt tttctctcag gcatggagag ttaaattcgc gaattgctgt ttttcttata
     2401 tcagcaatga aaaaatcttt ttcaacgttt agttggggga gtagctcttt taatgagaat
     2461 gttctcaata aggtaaagat aaatatacct acgtttaata atgttcctgc atataattat
     2521 atggtaaagt atatagaaga gctggaagcg gcccatatag aagagctgga agcggcccat
     2581 atagaagagc tggaagcata cctgatagct gctggactga gtgattacca actaacggaa
     2641 aaagattatc ttatcctgga atattttaaa aatattgaat tttcaggctt tcctgttact
     2701 gagttattct cagtgtgcaa tacaggaaat atattgtcta gagacatcat agaaaatagt
     2761 ggggatgttc cttatctttg tgctagccgt gagaataact ctgttagttc ttatatcgcc
     2821 tatgatgact cactcttgga aaaagggaac tgtattttta ttggtggtaa gacattcgtt
     2881 gtaacttatc aggagcgaga ctttttttca aacgacagtc ataatttgcg actctatgtt
     2941 aacaatgagg atggaaggac aaagtttgct tatttgggca ttatttcatg tatttataga
     3001 tctatttcac ataaatattc atggggggat agtgttagta atagaaaaat taagaacgat
     3061 gttatttggt tgccagtaaa aaataagtca cctgactttt gttatataaa tgaccttatt
     3121 tcagttgttc ataagcttgt cattaaagat gttgtccagt acgcagaacg caaaatgaac
     3181 gcatacaggc aagctataac aagtcgcggt tatgatgatt ctggtgtcag ttaaaaaaca
     3241 ttttacaatc gtaaaaatcc tcatatcatg ataagaatgg ttacattgcc acacagccga
     3301 acccgccgat gcgcgggttt tttgtatccg gaatcctgtg agttatacgg aaagtacaca
     3361 gaaaggaagg tgcgaccgca attaataaca aaatcttaaa aatcgcatat ggcactatta
     3421 gttttctaaa tattgtatat tttgagtatt gtaggatatc ccctgtaacg aagtttgcgt
     3481 aacagcattt tgctctacga gtttgccagc ctcccccagt ggctggcttt ttttgtatcc
     3541 gttcaacagg aatgttacat acctcacaat taagtcagtt gaaggttgtc tgcccggatg
     3601 agaatttgtt agaaaaaaac tgcatggtga atccccctga gcggcggggc atatcagcgt
     3661 cacaggtgtt tctgttttac ctctatcctt tctgtgcggg ttcaggtgct gacactgaac
     3721 tcaccgggag gcacccggca ccatgcagta tacagagatt aggcatatac cagggcccct
     3781 catagcaggg gcctttttac atgcaaaaaa aagccgcccc gggaagagcg gttggcaaga
     3841 agaaaagcaa tatgaacaat taattaacgc tgcgaataat accttacagt aatcaccctg
     3901 cgcaactgtg aggagtgtat ttttttgcgg gtggttgtct ttttcccttg tgtttcggga
     3961 cttccgttca ctccttatct tattcagtac attatcccgg ccgggaggat tcatggcatt
     4021 taaacactat gacgtggtca gggcggcgtc gccgtcagac cttgcggaaa aactgactca
     4081 aaaactgaag gaagggtggc agccttatgg tagtgccctg atttcgacag ctggttatgg
     4141 tgcggagttt atccagcctg ttgtgagtga cagggatatc tcatcacctg cggagccagg
     4201 cagtcacccg accgtttcaa cgaagcctgc agccgaacca gaatattact atgtgatcgc
     4261 gcttgctggt cagtccaatg gtatgtcata cggtgaggga ctgccattac cggatacatt
     4321 cgacagccct gatccacgaa ttaaacagtt agcgcgtcgc agtacggtga caccgggagg
     4381 tgctgcttgt acctataacg atattatccc ggcagatcac tgtctccatg atgtggagga
     4441 tatgacaggg cttaatcatc ccaaagcgga ttcatcgaaa ggtcagtacg gctgtgtggg
     4501 acaggggctg catatcgcca aaaagctgct gccgtttata ccggcgaatg cgggcattct
     4561 gctggtaccg tgctgccgtg gtggttcagc tttcaccacc ggggcagatg gcacatacag
     4621 tgacactact ggcgcctcag agagttccac ccgctggggg gtggacaagc cgctgtataa
     4681 ggacctcatt ggtcgtacaa aagccgcgct ggcgaagaat ccgaaaaatg tgctgcttgc
     4741 cgttgtgtgg atgcaggggg agtttgattt cagcggcacg ccagcgaatc acacggctca
     4801 gtttggcgca ctggtggata aataccgtgc ggaccttgca gatatggtgg gacagtgtgt
     4861 tggtggttct gctgacggtg ttccctggat ttgtggagac acaacatatt tctggaagca
     4921 gaagaacgaa acggcgtacc agacggtgta cggtagttac aaaaacaaaa cggaaaagaa
     4981 tatccatttt gtgccgttca tgaccgatga gaacggggca aacgtcccga cgaacaaacc
     5041 ggaagaagac ccggatattc cgggtatcgg gtattacggt tcaaaatggc gaacggacag
     5101 aagcacctgg acatctcagg acagggccag ccatttcagt tcatgggcac gacgtggaat
     5161 tatttcagac cgtctggcaa cggccatact gagctgcgcg ggtaagtctt ctgcgtttgt
     5221 taatggtact gccggggtgg ttgttccaga cagaccggtt accacctcag agtctgtaat
     5281 tttttacgat gccaaaaaag cttcagacaa tcagctgaaa ccttatggct gggacggtat
     5341 ggatggcaga cgcacactgg ttgatgacag cggcaataaa gctctgcgaa ttgagaaaaa
     5401 taacagcgcg aaatcctggt caatgtactg tgatattgct gcagacaagg caaaactgtt
     5461 actggaaaaa ggcggggaaa ttgctgtccg gtttaaaatc cccgaaaacg tcaatcttga
     5521 aacaaccaga aacaagtatg cctttggttt gtactggcga atagcggaat ggccgggtga
     5581 gggtggtgaa ggctatctga gttctttctt tgtccagaca gataaagcca gtattgatgt
     5641 tgcataccat catacaggta atcaacaaaa agaacttggc acgtttggcg cattcgacca
     5701 tgactggcat acgcttgcat ttaaatttaa gggcagtaac agcattaatg ttactccggt
     5761 gcttgatggt gtggatggac aggcgtttga cctggtgaaa tgggccaata ctgctaatga
     5821 actcaacagg tttgtcatta cggatattac aggtagtgca gaaacctacc ctgtacttat
     5881 tgatacggtg gaagttaaag caaacaaagc tggagcagcc gcataattgc taaaaaaagc
     5941 cgccagcggc aggaatggaa gctggcggtg gtaatcccaa tggagaatgt aaagaaaaga
     6001 tgctttcgta catcggttgt tttttaaatg aaaacagttc tcattgtcaa ccataacggt
     6061 aagaaattat gacatttatt catcaggtga tgctgtactt ctgtacggcg gtctgtgtgc
     6121 tgtatcttct ttcgggtggg tacagggcag tgcgcgattt ctggcgcagg cagattgata
     6181 aaagggctgc tgagaaaatc agcgccagtc agtcagccgg aactaaaccc gaagagccgc
     6241 tcattcctta acaacccctt tcagcgagaa aatcctatgt cagaaataaa acccctggtc
     6301 actgcagagg cagtgaagga cgtcattcgc tctgaagaag tcagaagcgc actgaaacag
     6361 aaacttcgcc agaatctgga ggaacgcctc gatgctgaag tggatgccat tctggatgag
     6421 ctgctcggcg taccggcaga gccaccgact gaagcgggag ataccaccgc agagaacggt
     6481 aaacctcagc ctgaattacc ggtagccgat gcaactgaac cgcaacctga aacggtcatg
     6541 atgctgtaac gggctgtcag ggccgccagt aaaccactgg tggtcttttt attgttgtga
     6601 gcttccgttg cgcgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc
     6661 atacaccacg tcagcggtgg ggacgggcta ctggttcctg cagttgctgg acagggtttc
     6721 cccgtctcag tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacgta
     6781 cctgacgaac ctgtatttca aaattaaaga agaccggcgt aaggcggcgc ggggagagta
     6841 agctgatgag caggaaattc cgctatggtt tatcagcggt cgttctggcg ctgattgctg
     6901 caggtgcttc tgcgcctgaa atcctcgacc agtttcttga cgaaaaagaa ggtaaccaca
     6961 ccacggcata ccgtgatggt gcgggtatct ggaccatctg tcgtggtgcc atcatggtgg
     7021 atggtaagcc tgtcctcccg ggcatgaagc tgtcgaaaga aaaatgcgac caggttaatg
     7081 ccattgaacg tgataaggca ctggcatggg tggagaaaaa catcagagtg ccactgactg
     7141 aaccccagaa agcggggatc gcgtcattct gtccgtacaa cattggcccc ggtaagtgtt
     7201 tcagctcaac gttttaccgg aaactgaacg ccggagaccg taaaggcgca tgtgctgaaa
     7261 ttcgtcgctg gatatatgac ggtggtcagg actgccgtat tcgctcaaat aactgttatg
     7321 gtcaggtatc ccgtcgtgac caggagagcg cgctggcgtg ctgggaaatc gacagataag
     7381 cagaatattt ttctgaaaaa tgaggtttgc ttacatggac ggataacacg aaatcctgcg
     7441 aactggcaaa atgtaagtga ataaaagtaa aaaccccgtt tgttggcagc aagcggggtt
     7501 ttgtgtttct gaccttggat aaggcaaggg agaaattatg ggtagggagg tgctttccct
     7561 gtgaggaagt ataaaagatt ctttctgagg ttgtccatta tgaaaggcat tgaagtggag
     7621 acgccagcca gtctggattt aacaagagcg gcagcttttg ccattcgtat tgtggccatt
     7681 gctgttctgg tctgggcaat ccgttggtgg tgatatgaac cgtgttctgt gtgtggtgat
     7741 tattgtcctg ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt accgcgataa
     7801 cgccatcacc tacaaagcgc agcgagataa aaaagccaga gagctggagc tggcaaacgc
     7861 aaccattact gacatgcagc agcgccagcg tgatgttgct gcgctcgatg caaaatactc
     7921 gagggaatta gccgatgcga gagctgaaaa tgaaactctt cgcgctgacg ttgccgctgg
     7981 tcgtaagcgc ctgcgtatca acgccacctg tcccggctcc gtgcgtgaag cccccaccac
     8041 ctccggcgtg gataatgcaa ccggcccccg actggcagac accgctgaac gggattattt
     8101 caacctcaga gaacggttga tgacaatgca gaagcagctg gaaggggcgc aggaatatat
     8161 ccacactcag tgcctgaatt aacagagcca gcatgaatgc tgtttatctt ctgctgagat
     8221 aaagattgcg aaatgcgaca atttgttgca tatttaaggg gcgctttcca gaagcgtatg
     8281 tgttctcacc gcatgaagta caaccacata gaggtcaccc ataatggcaa caagcattcg
     8341 tttagacgat gatttcgtca gtgatgtaaa ggtttacgct gatgctgcaa gtcgcagcat
     8401 accaaagcag attgagtact gggctaagat tggtcgtata gctgaggata accccgatct
     8461 gccatacagc ttcattagcg aagttctgtt gtcacaaagc gaaatcagac acaacaaggt
     8521 aacgcgttat gtcagaaaaa cagacaggtc gtgaaattga tgtttatcag agtcggcgtt
     8581 tcgaaaagtc gctgactaaa ctcccggaag cgcaactgaa agttgtggaa gatgagatcg
     8641 acagaataat cgacaatcct ctgttgggaa cgcaaaagaa aggagacctc agttttcttc
     8701 gtgtgcataa atttcagctg aataatcaac aggtacttct ggggtacagc tggattgacg
     8761 ataagctgga gctttacctt ctgagcattg gtccacatga aaatttttat actgaccaga
     8821 aaaaacaccg aaaagcggat ttaaagctca tcggttagtc cggacatcat gcataccagc
     8881 ccacgtaaac gcgtgggctt tttcatatct gaatttaacc gcgcaccgca gcgcacaata
     8941 accaccgaac ctgccccttt ggaatgagcc tttgaggata ccagttagtg ctggcgagcc
     9001 tcggtgggct ggtttcctgt gcggcaaagg ttcatttcaa agagcaggta cacgctatga
     9061 actatccgac gattgttgac ggtattgatt ttcgccaact ggtaacagcg gcagatggcg
     9121 aaccaataac agactctttt cagatcgcta aggcgtttgg taagcgtcat gcggacgtat
     9181 tgagggcgct gaaaaattgc cattgctctg aagatttccg gagagcgcat ttttgcgttg
     9241 ccgaaaaatc aatgagttag ggattttcga caagaagcag atttactacc gcatggactt
     9301 tagcggcttc gttatgttgg tcatgggatt taacggggca aaagctgatg ccgttaaaga
     9361 agcctatatt aatgccttta actggatgtc cgctgaactc cgtaagttca gtgaaagtta
     9421 tgaagctgaa cgcaacgctg taatgctgga atacatgaaa gagaaagatg ttgccagtat
     9481 gtcaggtcgt ttgctcaatc gctggggcag ggtgaagaag cctcagttgc tggcaaggct
     9541 ggacaggctg gagcaacagg gacagattgc gttacccgga tttgataaag gcatttcagc
     9601 ctgacataac catgcgccgt atcgtcgccg tattcccgca taaacagaga ccgcagccac
     9661 cttatctgcg tgagtgtgcg ggggtaatca aaaacgatgc acaccgggtt ttctcatttt
     9721 tcacgagatg ggagcgattt cccgcgaagc cgcctgtccg gtgcggtggt ggaagaaacc
     9781 ggataaaaca accgcattgt acaaatatcg atcaaacatg gcgctgctgt gtgaattctg
     9841 aaaaatcaca gcggccattg tgcatcagtt tttaacagag gacgtcagaa agtgacatgg
     9901 caaagctgga ctggaaaaag ctggagcagg cattccgacg cgaacatgcc gaaacgggaa
     9961 taacattact ggactggtgc cggaagaaaa agattaatta caacaccgcc agaacccgta
    10021 taaaaatggg caaaatcgat catgaaattg atcataaaac cgatcatgaa atcgatcatg
    10081 acatctcaga tgaagaaccc tgcaatgacg cgggttccgg cgatgaaaaa tgcgcaaaaa
    10141 actctgaaaa aaactgcgca aattcggcag aaacgaaacg caacagcaga tcgggcaatc
    10201 ctcaccccgt tgcccggttt agcgaccgta acacccacgc cgtaagacac cgtggatatg
    10261 cgaagtatct tgaggcagat aacctcatgg atgatgcgtc cgacatggtg ctgttcgatg
    10321 aactggtgtt cacccgggcc cgcgcacttt cagtaactaa ggcacttaaa gggatgttcg
    10381 ccgacctgga agaggcaact gacgtggaaa cccgcgttgc tctttacgac aaaatactca
    10441 aagctgaaca ggcccttgac cggaatattg cccgtatcga gtcaattgaa cgctcattgc
    10501 tgacgctgga cgtcctggct gagacagcac caaaacttcg tgctgaccgg gaaagaatca
    10561 acgccgccag agataaactc agagcggaaa ccgatattct gaccagccag cgccggggtg
    10621 ttattacgcc agtcagtgac atcgtgtcat cgctgcatga aatgagtaat tcggggagac
    10681 tggatgacat tccggaagaa taaaacgcga tgtgatgaac ctgcagaaat gactgagacc
    10741 gaacaacgtc tgttcattat gacaaaactg agcaatccct ggtggcgcct caatcatctc
    10801 tacaaaatac agaacgaaaa aggtgaactg gtcaccttca gaatgcgacc ggcgcaacgt
    10861 cagttgtttc gtaacatgca taataaaaac attatcctga aagcgcgcca gctgggattt
    10921 tccacagcca ttgatattta tcttctcgac caggcattat tcattccgca tctcaaatgc
    10981 gggatcgtcg ctcaggataa acaggctgcc agtgaaattt tccgcacaaa aattgctgta
    11041 ccgtttgatc atctccctga ctggctgaga gcctcattca ccatcgttga acgtcgtagc
    11101 ggtgccagcg gtggctatat cctgtttggt cacggctcga gtattcaggt ggcaacctca
    11161 tttcgctcag gtacggtgca gcgcctgcat atctcagagc acggcaaaat ttgcgcgaaa
    11221 tatccggcta aggcgaaaga gctgcgaacc ggtacgctta atgccgtctc tgatgaatgc
    11281 attatttttg atgaatccac ggctgaaggc gtgggtggtg atttttacga gatgagtaac
    11341 cgtgcacagg agagcactgc atcaggctta ttgctgacgg cacaggatta taaattccat
    11401 ttttacgcat ggtggcagga tcctaaatac agcgccagag tgcctgaaag cgggctgaag
    11461 ctgtcacggg aaaaaatgac gtatttttct gcggttgaga aggcaatgaa catcacgctt
    11521 accgatgagc agaagcagtg gtacatcagt aaggaaactg aacagcgtga ggaaatgaag
    11581 caggagtttc cctcaacgcc acaggaggcg tttctgacgt ccggacgacg tgtgttcagt
    11641 gccgaaagta cgttgcaggc agaatcattc tgttcgccgc cgatgattgt ttatgacatt
    11701 gaacctgtta caggagcgaa gaccaaagct cagtcgctgc gtgaaggaaa taaaaacgag
    11761 ttgcagcgga cgctgatgaa ttatctgctg gtatgggaac tgccggatcc ggatgaagag
    11821 tatgtttgtg gggcagatac tgccgaaggg ctggagcacg gagaccgctc atcgctggat
    11881 gttgtcaaac gcagtaatgg tgagcaggtg gctcactggt tcgggcatct cgatgctgaa
    11941 ctttttgctc atctcatttc gcaggtctgt cgtatgtata acaacgcgtt tgtggggccg
    12001 gagcgtaata atcacggaca tgcagttatc ctgaaactcc gggaactcta tccgacacgt
    12061 tatatctaca acgaacagca tcttgaccag gcatatgacg acgatacgcc ccgccttggc
    12121 tggctgacaa cccgtcagag caaacctgtt ctgaccgaag gaatgaaaac gctcctgaat
    12181 aatggaatat cagggatccg ctggtcaggc acattatcgg aaatgaacac ctacgtttat
    12241 gacgcgaaag gctccatgaa tgcacaggaa ggctgctttg atgatcagct catgagctac
    12301 atgattgccc aggagatgcg cgccagaatg ccggtgaggg taaaacagaa aacggataaa
    12361 cgcagaacca cacactggat ggcacactga tgaaaaatga aattaacacc acagcgatga
    12421 aaaacgatca tggatccacg ccgcgttttt ctcagcgtca gttactgtca ctttgttctg
    12481 atattgacag tcagcctctc tggcgtgatg ccgcaaacaa agcctgtgcg tattatgatg
    12541 gcgaccagct ggcaccggaa gttatccagg tactgaaaga tcgcggtcag cccatgacca
    12601 tccataacct cattgccccc acggtagatg gtgtactggg aatggaggca aaaacaagaa
    12661 cggacctgat agtgatgtca gacgatccga acgatgaaac agagaaactg gcagaggcca
    12721 ttaatgcgga gtttgctgat gcgtgccgtc ttggcaacat gaataaggcc cgctccgatg
    12781 cctatgcgga acaaatcaag gcggggctca gttgggtgga ggtcaggcgc aacagcgatc
    12841 cgttcggacc tgaattcagg gtatccacag tcagcagaaa tgaggttttc tgggactggc
    12901 tcagccggga atccgacctg agtgactgcc ggtggctgat gcgtcgccgc tggatggata
    12961 ccgatgaggc aaaagccacg ttcccgggaa tggctcaggt tatcgattat gctattgatg
    13021 actggcgtgg ttttgtcgat accacgatta ctgaaggcca gcccagtccg ttgatgagtg
    13081 catgggaaga gtatcagtca tgggatcgca gggagaatga atggcttcag cgtgaacgcc
    13141 gtcgtgtgct gcttcaggtg gtttattacc gtacattcga gcgtcttccg gtgattgaac
    13201 tcagtaatgg acgggtggtg gcttttgata aaaataatct gatgcaggcg gtggctgtgg
    13261 catccgggcg ggttcaggtg aaagtcgggc gggtaagtcg tattcgtgaa gcctggtttg
    13321 tgggcccaca ctttattgtg gatcgcccct gtagtgcacc gcaggggatg tttccgctgg
    13381 ttcctttctg gggataccga aaggataaaa caggggagcc atacgggtta atttcccgcg
    13441 ccattccggc acaggatgag gtgaattttc gtcgtatcaa gctgacctgg ttgcttcagg
    13501 ccaaacgcgt gattatggac gaggatgcca cccagttgtc agacagcgac ctgatggagc
    13561 agatcgaacg tccggatggc attattaaac tgaatccggc ccgaaaaaat cagaaaagtg
    13621 tcgcagatgt ttttcgggtt gagcaggatt ttcaggttgc cagccagcag tttcaggtca
    13681 tgcaggaatc ggaaaaactt atccaggata ccatgggggt ttattccgca tttctcgggc
    13741 aggagtcagg tgcgacgtca ggcgtggcta tcagtaacct ggtggagcag ggggccacaa
    13801 cccttgcgga aatcaacgat aactaccagt ttgcctgcca gcaggtggga agactgttgc
    13861 tggcttatct tctcgatgac ctgaaaaaac gccgtaatca tgcagtggtg attaatcgcg
    13921 atgatcgcca gcgtcgccag accattgtcc tcaatgctga aggtgataat ggtgaactga
    13981 ccaatgatat ttcaaggtta aatacacata ttgcgctggc gcctgttcag cagacaccgg
    14041 catttaaggc acagcttgca cagagaatgt cagaggttat tcaggggctg ccgcctcagg
    14101 tgcaggctgt tgtgctcgac ctgtgggtta atcttctgga tgtgccgcag aaacaggagt
    14161 ttgttgagcg tattcgtgct gcgctgggga cgccaaaatc accggatgaa atgacgccgg
    14221 aagaacagga agtagcggca caacaacagg cacttcagca acaacaggca gaactccaga
    14281 tgcgcgagat ggctggcaga gtggcaaaac tggaagctga cgccgccagg gcacatgctg
    14341 ctgcacaacg ggataatgcc agtgcacagc gggaagtcgc cctgacacag gggcagcgtt
    14401 atgtggatgc gcttaaccag gcacatacgg cagaaatcat taccggcata caaaatatgg
    14461 aacaggagca ggacgttctt cagcaacaga tgctgtatac gttacaacag cggatgaatg
    14521 aaatgtcgct ctgaaaactc tggcttcaac tgaaccccgt catcgtacgg ggttttttgt
    14581 ttccggaggt aagcgttccg ggagcggtgc gcttattcgc gggggcagcg ataagcctta
    14641 tttactcaac cattcggatc tgtccgataa acagaccatg cggagttatt tatggatttt
    14701 gaatttacgg gtgaagaaac cccggaacaa ctggaaaaaa tgctggaagg acttggggat
    14761 gtggatattg acagtcacgc acaggacgtc gtgacggaag ataccacgga aaaacatgcg
    14821 gatgaggaaa cacagactca gacgggcgat aacaatgtgg caccgacgcc ggatgccagt
    14881 gtggagcaga cgcaggacgt gaaggagccg gaagcgaagg gggtgctcac ccgcgacggt
    14941 aaacacgtca ttccctatga agtccttgag gctgaacgtt ccggtaagca acgggccgaa
    15001 caggaagccg cacttcttcg tgggcagata gctgaagaaa aacgcagggt ggaactgctg
    15061 acgtctcaga tccaccaggc cggtatgaag cccacaccgt taccggaaaa cgaaaaaatt
    15121 tctgatgagc agattgcccg tatcagggag atgtatccgg aaattggtga cgcggtggct
    15181 tcgctcatcc gtaaaaataa ctatctccag tcccgtgttc agcaatcaac acagcaggca
    15241 gaaggtaatg gtggtgagga tttatcaccg gttcttgatg cgatgaatgc cgtgccggtg
    15301 ctgaaaacgt ggcaggagtc cgatccggat cgcttctcgg ttgctgtatc catcgacggg
    15361 aagctccaga atgaccccgc atggaaagac aaaacgctca ctgaacgttt cgctgaagtg
    15421 gcccgtcgta cgcaggttgc tttcggtgaa gtcagtgagt cgtctgctga caacaaggca
    15481 gacaaaacgg atatccggaa aacggcggaa gagaaagtga agaccgctga acaggagcag
    15541 gcagtaccag cttccccgtc agatttaggc accacggcct ccgtcggaac cggtgataat
    15601 tttgaacggt tacttggcgc ttcgcattca gaggcagagg cgattatgcg cggtatgacg
    15661 aatgctgaaa tagacgcact tctggagaag ctcgggtaac ttactgaagg agtactgaag
    15721 taatgacgac tgtaacatca gcccaggcga ataagctgta tcaggtggcg ctttttactg
    15781 ctgccaaccg caaccgctcg atggttaata tccttactga acagcaggaa gcgccaaaag
    15841 cggtttcgcc ggacaagaaa agcacgaagc agaccagcgc aggtgcgccg gttgtccgta
    15901 tcacagacct taacaaacag gccggtgatg aagtgacctt cagcatcatg cacaaactct
    15961 caaaacgtcc gacgatggga gatgagcgtg ttgaaggtcg tggtgaggat ctcagccatg
    16021 ctgacttctc cctgaaaatc aatcagggac gtcacctggt ggatgcaggc ggacgtatga
    16081 gtcagcagcg cacgaagttt aacctggcat cctctgccag aacgcttctg gggacgtact
    16141 ttaatgacct gcaggaccag tgtgcgatag tgcatcttgc tggtgctcgc ggtgattttg
    16201 ttgctgacga cactattctg ccgacagcgg agcaccctga attcaaaaaa atcatgatca
    16261 acgatgtact gcctccgaca catgaccgtc acttttttgg cggtgatgcg acaagctttg
    16321 agcagattga agcggcagat attttttcta ttggcctggt ggacaatctc tccctgttca
    16381 ttgacgaaat ggcgcatccg ttacagccgg ttcgtctgtc cggtgatgaa cttcacggag
    16441 aagatccata ttacgtcctg tacgtcacgc cgcgtcagtg gaatgactgg tacacctcga
    16501 cgtccggtaa ggactggaac cagatgatgg ttcgtgccgt gaaccgtgca aaaggtttta
    16561 atcatccgct gttcaaaggt gaatgtgcga tgtggcgcaa tatcctggtt cgtaagtatg
    16621 cgggtatgcc gatccgtttc tatcaggggt caaaggttct ggtatcagag aataacctga
    16681 cggcaaccac gaaagaggtc gctgctgcaa ccaatattga ccgcgccatg ttactggggg
    16741 ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg tcacttcaac atggttgaga
    16801 agaaaacgga tatggataac cgtactgaga tagcaatcag ctggatcaac ggtctgaaaa
    16861 aaatccgttt ccccgagaag agcggcaaga tgcaggatca cggcgtgatt gccgttgata
    16921 cagcagtgaa gctctgattt tttcctttcc ccatgccggg ttttcgcccg gctttttcag
    16981 gagtcattaa ttatggcaaa gactatcctt gccccgtcac tgagtgaacg ggtctatacg
    17041 ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta cggtgaatgc tgcggaagcg
    17101 gacagtgtta ttcatcttct ctcactgcca gttggtatcc gtatcaactc actccagctg
    17161 gtttcaacgg gcggtctggg tactgcaacc gtcagcgtta agtccggtga gcatgttctc
    17221 atcgataaca gcgaagctgt ttctgcaaaa tttgccagat atgtgccagt ggagccgtac
    17281 accacacagc gtgacgggga actggttact gtcaccatta agactgccgc tgcaaccggt
    17341 acgctgagtg ttctgttgcg ttataccgtg gtgggatact gattaaaacc ttccggcccg
    17401 cgtcatgcgg gcttttttta tcgggggaat ttatatgagc gagaaaattg ccgttgtcta
    17461 tatcggccca aaacccgtga aaaaggacac cattaccgga agtcgcacgc tgttcccacg
    17521 tcttgagccg gtgcatgttg acagcgcgat ggcctggcaa ctgctggggt ttccggatgt
    17581 ctgggttcgt catgaagagc ttgatgatgt tctgaaaaag caacaacaga atgagcagtt
    17641 gcggcaggca cagcaggcgc aggaaagatc gcttgctgcg cgggcagaag cggagaacag
    17701 ttttgttgtt tctgttaacg ggcaggaggt ggatttaagt aagctcacct cagcacggct
    17761 ggcgacgctg tgtgaggcag aagagctgga tattcacaaa gacccgaaag aaacggctga
    17821 ggcattccgt atccgggtgc gtgaggcatt tcgccgtcgt gttgcggaga ctgaacagca
    17881 tggcggaact gagtgatttt ttaccatatg tccgtcgtca tatcagcggt ccgctgaaca
    17941 ttatgatgac ggatgctctg tcaatggctg ccgtggcatt cagccgccag tcgttggtgt
    18001 gccgtcggga ggttactgtt gtaccggtgg caggaaaaga aatcgtgctt ccgtatgaca
    18061 aagatgatga ggagtgcgtt catatcatcc gtatctctga cgataatcat gagctttttg
    18121 tcggtcggga tgtggatatc agctccggac gctccctgcg atttgcctgt tctcccggtg
    18181 aggtgagcgt gctttatgcc gtcgctccga aagccggacg cagccagata ccggatgaac
    18241 tcctcacatg gcctgaagaa gtggcagcgg gggcacttga gcggttgttc atgcagactg
    18301 gtgtttcatg gtcagacccg ttacgcgcac agtatttttc tgtgcagttt tctgagggga
    18361 tccgtcgggc atatcgtcat acactggcga caagcccgta ctcttcatac cgcaaccctg
    18421 tacgcaggca gaggttttac tgatgacgac gattacagaa atcatcggac gtgtgaatac
    18481 acaactggtt gacccgatga tggttcgctg gcccctgcag gaattgtgcg attattacaa
    18541 tgatgctgtg agggcagtga ttctggcgag accggatgct ggcgcaagcc tggaaacaat
    18601 cagttgtgtt cctggcgccc gtcaggtttt gcccgatggt gtaatacaac ttcttgacgt
    18661 gatatgcctc agtgacggta gtgcagtcag accattatcc cgggaggtgc tggatgcgca
    18721 gtatcctgag tggcccacaa tgaagggcat tcctgaatgt tttatcagca acgacctgtc
    18781 cccgcgcgta ttctggctgt ttcctgcccc tgacaaagag ataagtattg atgcggtggt
    18841 aagccggata ccggaggcag tgtatgttct gacgcaggac gatgatacgc cagttccact
    18901 ggaagaggct tatgttaacc cactggtgga ctggatgttg tttcgtgctt tcagtaagga
    18961 tgctgccggt ggtgcagaat cggggctggc tgcgcagcat tatcagagtt ttgttgagca
    19021 acttgggatc aaacaggggg cagacagtgc attgtctgcc cgtaaaaaag tgtttaacgg
    19081 aggtggagtg tgagtgttgt tgtttcgggg acgctgaaat ctcctgatgg tgaggcgata
    19141 tcaggagcaa atattaccct gacggcgctg acagtttcac cggatgcgct cagcggcacc
    19201 agtgcgtcgg cagtgacccg tgaaggtgga tattacggaa tgacgatgga gccgggggag
    19261 tatgcggttt cggtgacggt gaaagggaag actgctgtct acggacgtgt gcgtattgag
    19321 gggaccgaaa gtacggtgac gctcaatatg ctgttacgcc gcagtcttgt tgaggtgagc
    19381 atacccggag agctgctgac agatttccgg cagatacaga ataatgtggc tgatgacctt
    19441 gccactattc gtcgcctgaa tgaagacacg gcgacaaaaa acactcaggc cacacagtca
    19501 aaagacagtg cagcagccag tgcgaagagt gcatctgaca gtgcaaagac ggcaaccagc
    19561 agggcggctg aagccggaca aaaagcgact gatgctactg aggccgcgac ccgtgcaatc
    19621 acagcagcgg ggaatgcaga ggaaagctcg acccgtgccg gagagtctga aaaagccgcc
    19681 ggagctgatg cagaaaaagc cagacagcat gctgaaaagg ccagactggc gcaggagagc
    19741 gccggagaga tccttaagcg ggcagaggct gccactgtca gtgctgaaga ggccagacgt
    19801 atggctgaga atgcacgggg gccccggggg cctcagggag aaactggtcc gaagggggat
    19861 gtcggtccta aaggcgaaac aggtccagtg ggccctcaag ggcccgcagg gccgaaaggt
    19921 gagcgtggtg acattggtgc tcagggggct gtagggcccg ctggtccgcg tggtgagaag
    19981 ggcgaacagg gggagcgagg accgcaggga ataccaggcc tga
//
LOCUS       DAZFFI010000081        19603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFFI010000081 DAZFFI010000000
VERSION     DAZFFI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.081"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..729
                     /gene="tssB"
                     /locus_tag="ACHJ12_003442"
     CDS             229..729
                     /gene="tssB"
                     /locus_tag="ACHJ12_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4991178.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            764..988
                     /locus_tag="ACHJ12_003443"
     CDS             764..988
                     /locus_tag="ACHJ12_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991179.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1039..2514
                     /gene="tssC"
                     /locus_tag="ACHJ12_003444"
     CDS             1039..2514
                     /gene="tssC"
                     /locus_tag="ACHJ12_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4991180.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2521..2934
                     /gene="tssE"
                     /locus_tag="ACHJ12_003445"
     CDS             2521..2934
                     /gene="tssE"
                     /locus_tag="ACHJ12_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4991181.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2938..4788
                     /gene="tssF"
                     /locus_tag="ACHJ12_003446"
     CDS             2938..4788
                     /gene="tssF"
                     /locus_tag="ACHJ12_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4991182.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4752..5834
                     /gene="tssG"
                     /locus_tag="ACHJ12_003447"
     CDS             4752..5834
                     /gene="tssG"
                     /locus_tag="ACHJ12_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4991183.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5859..7139
                     /gene="tagH"
                     /locus_tag="ACHJ12_003448"
     CDS             5859..7139
                     /gene="tagH"
                     /locus_tag="ACHJ12_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO4991184.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTISNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7136..7660
                     /gene="tssJ"
                     /locus_tag="ACHJ12_003449"
     CDS             7136..7660
                     /gene="tssJ"
                     /locus_tag="ACHJ12_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4991185.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7663..8994
                     /gene="tssK"
                     /locus_tag="ACHJ12_003450"
     CDS             7663..8994
                     /gene="tssK"
                     /locus_tag="ACHJ12_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4991186.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8999..9760
                     /gene="icmH"
                     /locus_tag="ACHJ12_003451"
     CDS             8999..9760
                     /gene="icmH"
                     /locus_tag="ACHJ12_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4991187.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9769..12534
                     /gene="tssH"
                     /locus_tag="ACHJ12_003452"
                     /gene_synonym="clpV"
     CDS             9769..12534
                     /gene="tssH"
                     /locus_tag="ACHJ12_003452"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4991188.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12531..13274
                     /gene="vasI"
                     /locus_tag="ACHJ12_003453"
     CDS             12531..13274
                     /gene="vasI"
                     /locus_tag="ACHJ12_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4991189.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13279..14691
                     /gene="tssA"
                     /locus_tag="ACHJ12_003454"
     CDS             13279..14691
                     /gene="tssA"
                     /locus_tag="ACHJ12_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO4991190.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14800..18234
                     /gene="tssM"
                     /locus_tag="ACHJ12_003455"
     CDS             14800..18234
                     /gene="tssM"
                     /locus_tag="ACHJ12_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4991191.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPQQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDLPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGANGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18245..19597
                     /locus_tag="ACHJ12_003456"
     CDS             18245..19597
                     /locus_tag="ACHJ12_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO4991192.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYVPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
BASE COUNT         4560 a         5414 c         5469 g         4160 t
ORIGIN      
        1 tgacgcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
       61 gatcgataaa aagtcctttt cttatttatt ttgaagcgta gaatgcgggg tgatgaagga
      121 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
      181 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
      241 tttgaaggca gcgtcgcgcc tcgcgagcgt attaatatat cgtatgtccc aaaaacagat
      301 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
      361 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
      421 ggtgcagtga tggctgaagc agccattggc ctcaatttta ccgtccccgc cacgctgaag
      481 ggaagcacaa cggacgatga gctgaatgtg gcgctgaata tcaagtcact ggatgatttt
      541 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
      601 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
      661 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg ccaggtgagc
      721 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
      781 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
      841 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
      901 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
      961 gtcgcactgg cagcgcgtac ttcactgacg gcgaaaagcc ggtttcagaa acacagctaa
     1021 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     1081 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     1141 ggttcggaag gttatgacgt tgcccgccag ggtgtgacag cctttattgc cagcatcctg
     1201 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     1261 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     1321 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     1381 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     1441 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     1501 ggcggtgaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     1561 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     1621 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     1681 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     1741 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     1801 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     1861 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     1921 taccgctggt gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     1981 gtgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     2041 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     2101 gactctgata atgcagcctt tttctctgca aactcagtac aaaaaccgaa gcactttccg
     2161 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     2221 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     2281 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     2341 aacccgcctg cagacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     2401 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     2461 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     2521 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     2581 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     2641 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     2701 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     2761 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     2821 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     2881 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     2941 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     3001 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc ggatccggat
     3061 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     3121 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     3181 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     3241 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     3301 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     3361 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     3421 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     3481 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     3541 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     3601 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     3661 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     3721 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     3781 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     3841 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     3901 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     3961 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     4021 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     4081 catcagatgg aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     4141 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     4201 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     4261 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg taaaaatgcg
     4321 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     4381 ggcagcctgc actggtccct actctccgcc atgaacctga attatctgtc attactggat
     4441 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     4501 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     4561 gatcgcctgt ttacgggtat tcccgtccgg ggactggcct ccacgctgta tctgcacccg
     4621 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     4681 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     4741 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     4801 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     4861 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     4921 cagaattatc cctgcgtgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     4981 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     5041 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     5101 tcgatcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     5161 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     5221 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     5281 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     5341 ccgggatcct ggcaaatccg ggacgatccc cggaaataat ctgtggcctg gtgtcccact
     5401 gctttgattt gtcagaagtg acgctgcaaa actggcaacg tcgcaaggtg gatattgaac
     5461 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     5521 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     5581 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     5641 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     5701 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     5761 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     5821 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     5881 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cggacgggcg gcgcgctgtc
     5941 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     6001 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     6061 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     6121 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     6181 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     6241 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtacgcca cactatccgg
     6301 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     6361 tccaggcact tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     6421 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     6481 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     6541 cagaaatggc gcaacgccac cttgcggtca cccccttact gcgcggtctt ggcggctcgc
     6601 ttaccattag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     6661 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     6721 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     6781 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     6841 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     6901 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     6961 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     7021 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     7081 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     7141 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     7201 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     7261 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     7321 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     7381 agccgactat gaccagatag ccagtacgcc gctaccggat gtgctgggta aaaactatat
     7441 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     7501 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     7561 atggaaacaa atcgagacgg tggaaggaac cggccaccat taccgcctgc tggtgcatgt
     7621 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     7681 aaggtgatgt ggcaggaagg gatgctgatg cgcccacacc atttccagca acagcagcgt
     7741 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     7801 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     7861 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     7921 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     7981 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     8041 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgcta
     8101 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     8161 ccgctttgcc gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt
     8221 atcccctcct gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta
     8281 cagggggcca ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag
     8341 agcggcatcg cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc
     8401 cggtttaccc atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt
     8461 gctgggttgc tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt
     8521 gatgtgtatg atcatcatga tctgaccaaa acatttaaaa cactgttacc ggaagtcaaa
     8581 cgggcgctac ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac
     8641 ggtatctggc aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg
     8701 gccgtggcgg caaacgtgcc ggtcgatcag atccagcgtc agtttatcca gcagtcgaaa
     8761 atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg tattccattg
     8821 cgtgccctta tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa
     8881 ctcgacaaga gcggacaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat
     8941 gtatccggca gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat
     9001 ggatgagggg tcactgtctc tgccaccatt taccggatac gacgaaaaaa gccagcgcga
     9061 ctatcatctc gcgcttcgtg gcaacagcct gaatccgatg atcgacgcgg ccacgccgct
     9121 actcggtatg gtaatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt
     9181 tgctcaggtg gtgacggatg ttcaggccgt ggagcaactg cttcaggagc agggctacga
     9241 accgggcgtg attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct
     9301 cggtaacggc tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca
     9361 caacgaagcc tggggtggag agaaagtttt catcctgctg gagcgcctga tccgtgaacc
     9421 caaacgttac caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg
     9481 gcgctacaaa gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgcctgta
     9541 ccacgtcctg cataaactac gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa
     9601 aacccagggc gggcgttatc agctcatcag tcgcctgacc attaaacata ttttttgtgg
     9661 tggcgttgtc gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac
     9721 gcaggacatc ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt
     9781 gatcttccca cgctggtaaa acggcttaac ctgttctccc gccaggcgct ggagatggcc
     9841 gcctctgaat gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag
     9901 atgctcgcca tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg
     9961 gaagagttgc gccaggcgct gacggtggag aactacacaa ctgcccgttc tgcggacagc
    10021 tacccggcat tttccccgat gctggttgag tggctgaaag agggctggct gctggcgtcg
    10081 gctgagatgc agcacagcga attgcgtggc ggcgtgttgc tgctggccct gctgcattcg
    10141 ccgctgcgtt atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt
    10201 ctgcaacagg actttgtgca atggacacaa gagtcggcgg aatcagtcgt gccggatgca
    10261 gacggtaaag gcgcaggcac actgacggac gctgctgaca ccctgcttgc ccgctacgcc
    10321 aaaaacatga ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac
    10381 gaaatcgacc tgatgatcga cattctctgc cgccgccgca agaacaaccc ggtggtggtg
    10441 ggcgaagcag gcgtgggtaa aagtgcctta atcgaagggc tggcgctgcg catcgtggca
    10501 ggccaggtgc cggacaaact gaaaaacacc gatatcatga cccttgacct gggcgcattg
    10561 caggccggag cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcggag
    10621 gtcatttcct ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc
    10681 gcaggcaacc agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc
    10741 ggcgagctga aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa
    10801 gatgccgccc tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa
    10861 gccaccatta ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt
    10921 gatgatgacg ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag
    10981 ttaccggaca aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg
    11041 tcgtcgccgc cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa
    11101 attcgccagc ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc
    11161 gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg
    11221 caccagcagc agacgctggt ccgggagatt attgcgctgc gccagcagtt actgggcgtg
    11281 gcagaggacg atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag
    11341 tcagagtcag aacaggataa taccggtgcc gtaccggctg atgaggccga cagagaacaa
    11401 ccggaagaga ccgctgaaac agtttccccg gtacaacgtc tggcacatct cactgccgaa
    11461 ctggacgccc tgcataacga ccggttgctg gtttccccgc acgtcgataa aaaacagatt
    11521 gcggcggtga ttgccgaatg gaccggcgtg ccgcttaacc gcctgtcgca gaatgaaatg
    11581 tcggtcatca ccgacctgac gaaatggctg ggtgacacca tcaaaggcca ggacctggcg
    11641 attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtcg tccgggacgc
    11701 ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga aaccgtcctg
    11761 caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa tatgtccgaa
    11821 ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta cgttggctac
    11881 ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt agtactgctc
    11941 gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc gttcgataag
    12001 ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt cttcttcctg
    12061 acgtccaacc tcggctacca ggtaatagtc gagcatgccg atgacccgga aaccatgcag
    12121 gaagtactgt atccggtgct ggccgacttc ttcaaacctg ccctgctggc gcgtatggaa
    12181 gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc cggaaaactg
    12241 gcccgtctgg ataacgtgct gcgcagtcgc tttggtgcag aagtggtcat tgaaccggaa
    12301 gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag gatgctggaa
    12361 tcggtcatcg atggcgacat gctaccgccg ctctcgctgc tgctgttgca gaaaatggcg
    12421 gctaacacgg cgattgcccg gattcggttg tcggcagtgg acggcgcatt tacggcagac
    12481 gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt atgagcaggt
    12541 tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc ttacccgcca
    12601 ctgccgcgcc cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc gccaccacga
    12661 ccccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg gagtccgcgg
    12721 cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg tcagggtttg
    12781 acggggcgct ggtcaaagcc ggttttgtgg gcgaggcctg gacccgcgcc acagagcagg
    12841 aaaaacgccg cgagggcaac accacagaac tgctggtgac gcaggtgccg ggggagcgcc
    12901 cgacggtggt gataaccacc ccggctatcg gtcatgttcc gccacgcccg gtgctgatgt
    12961 tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct ctggatgtcc
    13021 atgatatcgc cgtcactctg aatgccgaca accgggcatt gcgcagccac tggtttgtgc
    13081 gcgagaacgg caccctgctg gagtccagcc gggggttatc cgggattgat gaaattaagc
    13141 aactgttcgg cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc gccggaaagc
    13201 tgacctttaa cattgacggg ctggcaaggg cgattgctcc cctgcgcgat gcctgccact
    13261 gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg gatatcgcac
    13321 ctgctggaaa acctgccgga agagaaactg gcatcggcgc tgaaagatga caacccgaac
    13381 tgggagtata tcgacggcga aatcgtcaag ctaggatcgc tggcccacag tcaactggat
    13441 atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa ggattttcgt
    13501 ctgctggcgc atctgctgag aaccctgcaa cacgccggtg atcctcatct cgcattgcgc
    13561 ctgctggcgc tgtatgtgga gcattactgg gcggtggctg cgccgcagaa tatggcgcat
    13621 aaaaaacgct ttgccagcca ggtcattaaa cgctttgaaa caggtattga agttttttca
    13681 cagaacgccg ccacagcgca gcgcgatgcg ctgtcagggg aactggcaaa actggcgcaa
    13741 tgctggcagt cacataacgc cccggaactg gcacaggcta ctgatgatct ttttgccctg
    13801 taccagcgtg cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc ggcctccggt
    13861 agttcaccac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag tgctccggct
    13921 ccccaaatcg tcatcgacag tcacgacgac aaagcctggc gcgacacgct gttaaaagtg
    13981 gcggccattt tatgtgaacg ccagccggat tcaccgcagg gctaccgtct gcgccgccat
    14041 gcgctttggc agaacatcac cagtacgccc caggcggaaa gcgatggccg tactccactg
    14101 gctgcggtct ccgccgatat ggtggcagat taccatgctc agctcggtag tgccgatatg
    14161 gcgctgtggc agcaggtgga gaaaagcgta ctgctggctc cttactggct ggatggtcac
    14221 tgcctttctg cacagacggc gctgcgtctg ggttacaaac aggtggcaga cgccatccgc
    14281 gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct gtttaatgac
    14341 cacacaccgt ttatcagtga gcagacgaaa caatggctgg cagcttcgcc cgacgccaaa
    14401 gttgcaccgg ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg ttttgctgag
    14461 cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga tccccgcgat
    14521 cagtttcacc gccagtacct tgccgcacag ttgacggaag aggcggggct ggtacagctt
    14581 gcgcagcagc agtaccggat gttgttcagg atgggattgc agatgatggt ggcagactgg
    14641 gagccgtccc tgcttgagca gctagaacaa aaattcacgg cagaacaata aaagagaaca
    14701 ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa atcagccctg
    14761 cgaccggcga tgccccggtt taaagtctct gctttctggc tgctgatact ggcgtggatt
    14821 tttctgctgg tgtggatctg gtggaaaggc ccgatgtgga cgctgtatga agaacagtgg
    14881 ctcaaaccac tggcgaaccg ctggctggca acggcggcgt gggggattat tgccctggtg
    14941 tggcttaccg tccgggtgat gaagcgcctg caacagcttg aaaaaatgca aaagcaacag
    15001 cgcgaggaag ccgttgatcc gctcagcgtg gaactgaacg cccagcagcg ttatcttgac
    15061 cgctggctgc tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg gcagttgccg
    15121 tggtatatgg tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg cgaggggttt
    15181 ccgtccgaca ttatttatgc cccggagggc gcacgcggcg cagaacaacg cctgtacctc
    15241 acgccccatg tcggtaaaca ggcggtgatc tttgatatcg acggcacact ctgcgctccc
    15301 gctgatgcgg atatcctgca tcgccgtctg tgggaacatg ccctcggctg gctgaaagaa
    15361 aagcgcgcgc gccagccgct gaacgggatt attctgacac tcgatttacc cgatctgctt
    15421 accgcagaca aacgccgccg cgagcatctg ttacagacgc tgcgcagccg cttacaggat
    15481 atacgccagc atcttcactg ccagttaccg gtttacgtgg tactgacccg gcttgattta
    15541 ttacagggtt ttgccgccct gttccagtca ctcaacagac aggatcgcga tgcgattctg
    15601 ggcgtcacgt tcacccgccg tgcccatgaa aatgatgact ggcgaacaga gttgaatgct
    15661 ttctggcaga catgggtgga tcgaatgaat ctggcgttgc cggatctgat ggtcgctcag
    15721 actcacaccc gcgcgtcttt attcagtttt tcccgccaga tgcagggaag ccgtgaaccg
    15781 ctggtgtcac tgcttgaggg tctgcttgat ggcgaaaata tgaacgtgat gctgcgtggt
    15841 gtctatctca cctcttcgct tcagcgtgga cagatggatg atatattcac ccagtctgcc
    15901 gcccgccagt accggctggg caataaccca ctggcgtcct ggcccctggt ggacaccgcg
    15961 ccttatttca cccgcagcct gttcccgcag gcattactcg cagagcctaa tctggcaaca
    16021 gagagccgcg cctggctgat acgttcccgt cgccgcctga cggttttctc tgccacaggc
    16081 ggcgtggcag cactgctgct catcaccggc tggcatcact attacaacgg taactatcag
    16141 tccggcatca ccgtgcttaa gcaggccaaa gcctttatgg acgtgccgcc tcagcagggg
    16201 gaagatgact ttggcaacct gcaactgccg ctcctgaacc cggtacgcga tgccacactg
    16261 gcctatggcg actggggcga ccgcagccgt ctggccgata tgggactgta ccagggacga
    16321 cgtatcgggc cttatgtgga acagacctat ctgcaactgc tggagcaacg ttacctgccc
    16381 tcgctgttta acgggctggt caaagcgatg aacgccgcgc cgccggagag tgaagaaaaa
    16441 ctcgcggtgc tgcgcgtaat gcgaatgctg gaggacaaaa gcggacgtaa caatgaggtg
    16501 gtgaagcagt atatggcaaa acgctggagc gaaaaattcc acggccagcg cgatatccag
    16561 gcacaactga tgtcccatct tgactacgcg ctggctcata ctgactggca tgcagagcgt
    16621 caggcgggcg acggtgacgc catcagccgc tggacgccat atgacaagcc cgtggtatca
    16681 gcacagaaag aactgagcaa actgcctgtc taccagcggg tttaccagag cctgaaaacg
    16741 cgggcgctgg gcgttcttcc tgccgacctc aatctgcgtg accaggtagg gccaaccttt
    16801 gaccaggtgt ttacatctgc cgatgacaac aaactggttg ttccacagtt tcttacccgt
    16861 tacggcctgc aaagctattt tgtaaaacag cgcgatgaac tggttgaact gacggcgatg
    16921 gattcctggg tacttaacct tacccgcagc gtgaaataca gtgacgccga ccgtgcggaa
    16981 atccagcgcc agttgaccga gcagtatatc agcgactaca ccgccacctg gcgggccggg
    17041 atggacaatc tgaatatccg caattttgag tccatcggac aactgaccgg ggcgctggag
    17101 caggttatca gcggcgacct gcctttgcag cgggcgctaa ccgtgctgcg tgacaacaca
    17161 cagccaggcg tcttttctga aaaactctct gccaaagaac gggaggaagc cctggcagag
    17221 ccggattacc agttactcac ccgcctcggg catgaattcg ccccggaaaa cagtaccctg
    17281 gcagtacaga aagacaaaga aagcacgatg caggccgtgt atcagcaact caccgagttg
    17341 caccgctacc tgctggcaat ccagaacgcg cctgtaccag ggaaatcggc gctgaaagcc
    17401 gtgcagttac ggcttgatca gaacagcagc gatccgatat tcgccacccg ccagatggca
    17461 aaaacgctgc ctgctccgct caaccgctgg gttggcaggc tggctgacca ggcctggcat
    17521 gtggtgatgg tggaggctgt tcattatatg gaagtggact ggcgcgacag cgtggtgaaa
    17581 ccgtttaacg agcaactggc aaataactat ccgtttaatc cgcgttctgc acaggatgcc
    17641 tcactggatg ccttcgaacg cttctttaaa ccggatggca tactggatac cttctaccag
    17701 cagaacctga agctgtttat cgataatgac ctgagtctgg aggatggcga taacagcgtc
    17761 attattcgcg aagatattat tgcgcaactg aaaaccgcgc agaaaatccg tgacattttc
    17821 ttcagtaaac agaacggtct gggaacatcc tttgccgtgg aaacggtatc gctctccggc
    17881 aacaaacgcc gcagcgtact gaaccttgac ggccagttag tcgattacag ccaggggcgc
    17941 aactataccg cccatctggt ctggcctaac aatatgcgcg aaggcaacga aagcaaactg
    18001 acgctggttg gtgccaacgg cggcgcaccg cgcagtatca gcttcagcgg gccgtgggcg
    18061 cagttccgtc tgttcggggc cggacaactg accggagtac aggatggcaa ctttaccgtg
    18121 cgctttagcg tggacggtgg cgcgatgacc taccgtgtgc ataccgacac ggaagataac
    18181 ccgttcagcg gtggtttgtt cagccagttt ggtctgtcag acacactgta ctgaggaggc
    18241 agggatgaat agtaacggac tgacacaaac tatcgttacc ggcagtgacc cgcgcgggct
    18301 gccggaattc agcgccatcc gcgaagaaat taacaaagcc agccacccgt cacagcctga
    18361 gctgaactgg aaactggtgg agtcgctggc gctggcgatt tttaaagcca acggagtgga
    18421 tttacacacc gccacctact atacgcttgc ccggacacgg acccagggac tggcgggatt
    18481 ctgcgaaggt gcggaactgc tggcggcaat ggtaagccac gactgggata agttctggcc
    18541 gcagggcggc ccggcgcgta ctgaaatgct ggactggttt aactcccgaa ccggcaatat
    18601 tctgcgtcag caaatctcct ttgcggaatc cgacctacca ctgatctacc gcacagagcg
    18661 ggcattgcag cttatctgcg ataagctcca gcaggtggaa ctgaagcgcg ttccgcgcgt
    18721 ggagaatctg ctctatttta tgcagaacac gcgtaaacgg cttgaacccc agctgaagag
    18781 taacactgag aacgccgcac agaccacggt cagaacgctg atttatgtcc cggaaacaca
    18841 ggcatcttcc acaccagaag cggtagtgcc tcccctgccc ggcctacctg agatgaaagt
    18901 ggaagtgcgc agtctgacag agaatccccc acaggccagt gtgataaagc aaggcagtac
    18961 ggtaagaggg tttatcgcag ggatcgcctg ttcagtggct gtcgcctcag cattgtggtg
    19021 gtggcaggtc tatccggtgc agcagcaact gttacaggtt aacgacaccg ctcagggcgc
    19081 agcaacggtg tggatggcct cacctgaact cgaaaactat gagcgcaggc tgcaacaact
    19141 tcttgatacc tccccggtac agccgctgga aaccgggatg cagatgatgc gtgttgccga
    19201 cagtcgctgg ccggaaagcc tgcaacagca acaggcctcg acacaatgga atgaggcact
    19261 caaaacccgc gcacagagta gcccgcagtt gcgtggctgg ttgcagaccc gccaggactt
    19321 acatgctttt gcagatctag tgatgcagcg cgagaaagag ggactaaccc tttcctatat
    19381 caaaaatgtc atctggcagg cggagcgggg actggggcag gaaacacccg ttgagtctct
    19441 gttgacgcag taccatgatg cccgtgcgca gaagcagaat acagatgcgc tggaaaaaca
    19501 aattaatgag cgactcgaag gcgtgttaag ccgctggctg ctgctgaaga ataacgtcat
    19561 gccagaggcg gcaaccggta ccacggccga aaaataatta ttt
//
LOCUS       DAZFFI010000082        19526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFFI010000082 DAZFFI010000000
VERSION     DAZFFI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.082"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1035
                     /locus_tag="ACHJ12_003457"
     CDS             <1..1035
                     /locus_tag="ACHJ12_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4991193.1"
                     /translation="AASRGEFVEGISDPNDSKWVKGIQSRDAEDQNQQETEQNGQKAE
                     QNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCGAVMGDATYQETFNEE
                     SQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREITSTSRL
                     WHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDISTIEW
                     WLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILRRSYE
                     RQEIPCPWRYTNDRDVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAKYVSVIW
                     KKLFPNQADF"
     gene            1106..1357
                     /locus_tag="ACHJ12_003458"
     CDS             1106..1357
                     /locus_tag="ACHJ12_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4991194.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQALARPRRHLA"
     gene            1392..2666
                     /locus_tag="ACHJ12_003459"
     CDS             1392..2666
                     /locus_tag="ACHJ12_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO4991195.1"
                     /translation="MKYPTGVENHGGTLRIWFVYKGVRVRENLGVPDTVKNRRIAGEL
                     RASVCYAIKTGIFDYAKQFPYSHNLEKFGEARQNLTIGELAEKFLALKETEVARTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNSYLADNPFKGISPLKESRTIPDPLSREEFICLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDHFTVPKTQAGTNRVIHLI
                     KPAIDALQSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVTLLNTQLSEFAPTMPHNKAG"
     gene            complement(2860..3879)
                     /gene="rspB"
                     /locus_tag="ACHJ12_003460"
     CDS             complement(2860..3879)
                     /gene="rspB"
                     /locus_tag="ACHJ12_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO4991196.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3891..5105)
                     /gene="rspA"
                     /locus_tag="ACHJ12_003461"
     CDS             complement(3891..5105)
                     /gene="rspA"
                     /locus_tag="ACHJ12_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO4991197.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(5311..5637)
                     /gene="ynfA"
                     /locus_tag="ACHJ12_003462"
     CDS             complement(5311..5637)
                     /gene="ynfA"
                     /locus_tag="ACHJ12_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4991198.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            5772..6113
                     /gene="ynfB"
                     /locus_tag="ACHJ12_003463"
     CDS             5772..6113
                     /gene="ynfB"
                     /locus_tag="ACHJ12_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4991199.1"
                     /translation="MKITLSKRIGLLAILLSCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            6148..6708
                     /gene="speG"
                     /locus_tag="ACHJ12_003464"
     CDS             6148..6708
                     /gene="speG"
                     /locus_tag="ACHJ12_003464"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4991200.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(6711..7421)
                     /gene="ynfC"
                     /locus_tag="ACHJ12_003465"
     CDS             complement(6711..7421)
                     /gene="ynfC"
                     /locus_tag="ACHJ12_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4991201.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            7529..7834
                     /gene="ynfD"
                     /locus_tag="ACHJ12_003466"
     CDS             7529..7834
                     /gene="ynfD"
                     /locus_tag="ACHJ12_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4991202.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            8033..10459
                     /gene="ynfE"
                     /locus_tag="ACHJ12_003467"
     CDS             8033..10459
                     /gene="ynfE"
                     /locus_tag="ACHJ12_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO4991203.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            10520..12943
                     /gene="ynfF"
                     /locus_tag="ACHJ12_003468"
     CDS             10520..12943
                     /gene="ynfF"
                     /locus_tag="ACHJ12_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4991204.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12954..13571
                     /gene="ynfG"
                     /locus_tag="ACHJ12_003469"
     CDS             12954..13571
                     /gene="ynfG"
                     /locus_tag="ACHJ12_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4991205.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            13573..14427
                     /gene="ynfH"
                     /locus_tag="ACHJ12_003470"
     CDS             13573..14427
                     /gene="ynfH"
                     /locus_tag="ACHJ12_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4991206.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            14470..15084
                     /gene="dmsD"
                     /locus_tag="ACHJ12_003471"
     CDS             14470..15084
                     /gene="dmsD"
                     /locus_tag="ACHJ12_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4991207.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGAVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            15243..16535
                     /gene="clcB"
                     /locus_tag="ACHJ12_003472"
     CDS             15243..16535
                     /gene="clcB"
                     /locus_tag="ACHJ12_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4991208.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(16488..17183)
                     /gene="bioD"
                     /locus_tag="ACHJ12_003473"
     CDS             complement(16488..17183)
                     /gene="bioD"
                     /locus_tag="ACHJ12_003473"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4991209.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(17308..18528)
                     /gene="mlc"
                     /locus_tag="ACHJ12_003474"
                     /gene_synonym="dgsA"
     CDS             complement(17308..18528)
                     /gene="mlc"
                     /locus_tag="ACHJ12_003474"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4991210.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQPISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(18663..>19526)
                     /gene="ynfL"
                     /locus_tag="ACHJ12_003475"
     CDS             complement(18663..>19526)
                     /gene="ynfL"
                     /locus_tag="ACHJ12_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4991211.1"
                     /translation="FVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTN
                     RSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDT
                     LSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMA
                     MIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGE
                     AMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAAR
                     NFRIHLLNALR"
BASE COUNT         5120 a         4882 c         4757 g         4767 t
ORIGIN      
        1 gccgcttctc gtggtgagtt tgttgaaggg attagcgacc cgaatgattc gaaatgggtt
       61 aaggggatcc agagtcgcga cgccgaggac cagaaccaac aagaaacaga acagaacggt
      121 cagaaagcgg aacaaaatag cccaaatgcg ttacaaaacg agccagaaac gaaacaacct
      181 gaaccagtcg tgcaacagga agaggaaaaa gtttgtaccg catgcaatca gactggtggt
      241 ggtaactgcc ctgattgtgg cgcagtgatg ggtgacgcaa cctaccagga gacattcaac
      301 gaagaaagcc aggatgaagc ccgggaaaaa gatccggagg aaatggaaag tgccggactc
      361 ccgaacaagg agtgcaccga aggcgatcaa catgccaatg gcaataatga aacaggcgag
      421 acagcaaatc ccttaattaa ggtgaacggt catcgtgaaa tcacatccac cagcaggttg
      481 tggcaccatc tgatgattga ccttgaaaca atgggcaaaa atcctgacgc accaatagcc
      541 tcaataggcg ctgtattttt cgatccacaa accggagaac aggggcctga attcagcaaa
      601 ataattgata tgggtacatg tggcggcact gtagacataa gcaccatcga atggtggctt
      661 caacgctctg gcgaagcccg tgccgccatt ttagctgatc gaataccgct tgatgatgcg
      721 cttttacaat tacgggaatt tatagacgaa aactccggtg agttttttgt tcaggtctgg
      781 ggaaatggag ccaacttcga caacgtgatt ttacgccgtt catatgaacg gcaggagatc
      841 ccctgcccgt ggcgttacac caatgatcgc gatgtaagaa cgatggtcgc tctggggttg
      901 gtgatggatt tcgatgcccg caacgtcacc acatttgagg gtgaacgcca taatgccctg
      961 cacgatgcgc gttaccaggc aaaatacgtt tcagttatct ggaaaaaact gttcccgaat
     1021 caggctgatt tttaatgttc aaccgtcgcc ggttgtgact ggtattctgc aaccggcgct
     1081 catctgatgt aagagataaa aagcgatgag tgaagtaatc atgattgtct ctcccggcaa
     1141 atgggtatct gaagagcagt taattgcgct gaaaggaata aaaaaaggaa cactgaaaaa
     1201 agccagggaa aaatcattta tggaagggag agagtataaa catgtcgctc atgacggcat
     1261 gccgtgggat aacagcccat gtttttacaa tctggaagaa attgatcgct ggatcgagcg
     1321 tcaggcattg gcaaggccaa gacgtcacct tgcttaatcc aaaacaacac taatcaacga
     1381 gagaagctgg catgaaatat ccgacaggcg tagaaaacca cggggggaca ttacgcatct
     1441 ggtttgttta taaaggcgta agagtcaggg aaaacctcgg tgtccctgac acagtaaaaa
     1501 acagacgcat cgctggtgaa ctacgcgctt ctgtttgtta cgcaataaaa acggggattt
     1561 ttgactacgc aaaacagttc ccctactccc acaatctgga aaaatttggg gaagccagac
     1621 aaaatttgac tataggtgaa ctggccgaaa aatttctggc tctaaaagag accgaagttg
     1681 ccagaacttc actcaataca taccgtgccg tcatcaaaaa tatcctgagc ataatcggtg
     1741 aaaaaaatct tgcctcatcg attaataaag aaaaattact ggaggttcgt aaagagttac
     1801 tgactggata ccagatcccc aaaagtaact atattgttac acaacccggg agatcggctg
     1861 ttaccgtaaa taattacatg acaaatctta acgccgtgtt ccagtttggt gttgataaca
     1921 gttacctggc agataatccg tttaagggga tatcgccatt aaaggaatca agaaccattc
     1981 cagatcctct ttcgcgtgag gaatttatct gccttataga tgcgtgcaga aatcagcaag
     2041 ccaaaaattt gtggtgtgtt tctgtttata ctggcgttcg ccctggtgag ttgtgtgcac
     2101 ttggatggga ggacatagat ctgaaaaatg gaacaatgat gatcaggaga aatttagcaa
     2161 aagaccattt tacggtacca aaaacacagg cgggaaccaa tcgtgtaatt caccttatta
     2221 agccagcaat cgacgcactc cagagtcaga tgacactaac gagactgagc aaagagcata
     2281 tcattgatgt tcacctcaga gaatatggca gaacagaaaa acaaaaatgc acctttgttt
     2341 ttcaacctga agtgtcagcg agagtaaaaa attatggtga ccattttact gttgactcaa
     2401 taagacagat gtgggacgca gcaataaaac gagcaggact ccgccaccga aaatcatatc
     2461 agtcgagaca tacttatgcc tgctggtcgc taacagctgg tgctaatcct gcattcatag
     2521 caaatcagat ggggcatgcc gatgcgcaaa tggtgtttca ggtgtacgga aaatggatgt
     2581 cagaaaataa taatgcacag gtaacgctgt taaatacaca gctaagcgag tttgccccaa
     2641 ccatgcccca taacaaggcg gggtaaaatt aatttaatat ttatcaaatg gttactatgc
     2701 atgactcttg aaatccataa attcaagcgc agtgcccagc catcccgata ctgctgcttt
     2761 caccaaatcc ttagtgcttc tttcatgttt ttctattgtc ataatggtta tctctaaaaa
     2821 agaggtaaga tgcgtactac ttactcgccg ttattggtat tattcagaaa aagtgagcaa
     2881 gactttgcag caatgttttt gatcctgttc aaataaacta atggcatcag caacatgctg
     2941 gaaatcaaac gtatgggtaa ttaatttttc tggtttaatt aaccctttac ttaaccagtc
     3001 gataacaacc ggaaatttat ttgcatttaa gcgtgaagag aaaatagaga gttcttttcc
     3061 ggtaattcct tgctgaatca cttcagacgg ttcactggag aagcccatca atacaatacg
     3121 tgccgctgga gaagccagcg ttacggcctc tttcaggata gaaggatgac aagccgcatc
     3181 gataattaat gtcggcttga tgcctttttc agcgaaaatc tcgccaagcg gtgtctggct
     3241 gttattaatc gcccagtctg ccccgctctc tttcgctttt tccagtcgtt catcaatgcg
     3301 atcggcaaca atcacatttt taacgttata gacgcctttt aatacctgaa cgatcgtcag
     3361 gccgattgga ccggcaccat aaaccagaac ggtatcattt tcagtcggtt gaccatgacc
     3421 ggtaacgtta gccgcaatgg taaaaggttc gatcatcacc gcatattgat cggccactgc
     3481 ttcaggaatt ttccacgcat tttttgccgg aaccacggca tattcactga aaccaccgtc
     3541 agcgtgcaca cctaatacag caagtgtcgt acaaacgttc ggcttaccta tagagcacgg
     3601 atagcaatgc ccacagctga ccaccggatc gacagcaacg cgttcaccga ctctggcgct
     3661 ttccacacct tcacccaccg catcaatgac gccaaagaat tcatgaccaa tgacgcgcgg
     3721 atatttcgca aaaggattat gcccacggta aatatggcta tctgaaccac aaattccggc
     3781 aagtttcact tttactcgta cttcacccgc tgacggggtg ggtatttcac gttcgataat
     3841 cgacagttga ttcggttttt caattaaaat gcttttcatt accttactcc ttaccagttc
     3901 cacagcgtgc catcttccag acgtgcgact ggcagataag caggttcata gggatatttc
     3961 gccgccagct tttcatcgaa ttcgatgcca agacccggtt tgtctcccgg atgcatatag
     4021 ccgttatcga aagtccagtt gtgcgggaag acttcgagca tttgttcgga ataacccatg
     4081 tattcctgga caccgaaatt ggggacccac agatcaaagt gcagcgctgc agccatgcag
     4141 actggtgaca aatcggaagg accgtgtgag ccagtacgta cctgatacag cgaagcaaaa
     4201 tcggcaatcc ggcgcatacc ggtaattccg cctgcatggg tcagcgtggt gcggatataa
     4261 tcgatgagtt gctcttcaat cagttgtttg cagtcccaga tgctgttgaa gacttcaccc
     4321 actgcgatgg gtgtgacggt atgttggcga atgagacgga agcattcctg gttttctgca
     4381 ggcgtcgggt cttccatcca gaacatgcga taatcttcaa tgcttttacc aaagcgcgcc
     4441 gcttcaatag gcgttaagcg atggtgcatg tcatgcagca aatgttcatt aaaaccaaac
     4501 ttgttacgta ccgcgtcaaa caatttcggc atgaaatcga ggtatttctc cgtcgaccac
     4561 agctgctctt ccggccactg tcctttggtt gcgggttcat aagccagacc tttacctttc
     4621 gacatgccgt aggtggtttt cataccaggg attccgcact gcacgcggat ggctttgaat
     4681 cccagctcct gatgacgggc ataatcatcc agagcttcat caatactgtg accggtggta
     4741 tggcaataaa ccatcacccc ttcacgagac gcgccgccga gtaactggta aagcggcatg
     4801 ttggcagctt tggctttaat atcccacagc gccatatcaa ccgctgaaat ggccgacatc
     4861 gtaaccggac cgcgacgcca gtaagcacct ttatagaaaa actgccagat atcttcgata
     4921 cggtgcgcat cgcgaccaat aagctgcgga caaaggtgat cctgcaaata agaggccacg
     4981 gaaagctcac gtccattgag ggtggcatcc ccaaggcccg taataccgtc ctcagtggtg
     5041 atttttaatg tgacgaaatt acgccccgga caggtaacaa aaacttcagc ctttacgatc
     5101 ttcatgttcg attccttgca tcgcttgtcg tgatgcatga aatctacgca actgagctac
     5161 taccatacaa gtataaagat cgaaaaaagc cggagtgatc acaaaaaaag gcgtatattt
     5221 gcgctgtgaa tggttgacaa aagatgaaat agaatacctt ttgtcagctg acacttcctc
     5281 ttatcttatt gataaaatgg atttatgttc ctacgtgcgc ccccagcccg caacaatgat
     5341 caacatgccg caaagcgcaa tcaacgcacc cgtccagtca taaagagtca gtttcacgcc
     5401 atccacaacg cgcagccaca tcaacgccgt gcagacataa acgccaccat aagccgcgta
     5461 aacacgccca ctcgccgctg gatgcaacgt taacaaccag acaaacagcg ccagtgaaat
     5521 ccccgccgga agcaacagcc agatactggc gtttcgtttt aaccacaacc agggcagaaa
     5581 gcatccaata atttcacaca gcgcagtagc aaaaaatagt aacgttgttt taatcatctt
     5641 tgtctcttat tgacatcatg tatagttata gggcgacata atatcatcaa tataaacacc
     5701 ctcctggtac gttttgcgtc cgcagtggat ggtgtagaat cacctttaat cattcataca
     5761 gggaatgaat tatgaaaatc actctcagca aacgaatcgg cctgctcgct attctgctgt
     5821 cttgcgcact ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt
     5881 ctggcgacag tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca
     5941 cgcgcaatct gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg
     6001 ccgcttttga taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc
     6061 ccaatacttt gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta
     6121 ttgatttaag gaatgtaagg acacgttatg ccaagcgccc acagtgttaa gctacgcccg
     6181 ctggagcgtg aagatttacg ctatgtacat caactcgaca acaacgccag tgtgatgcgt
     6241 tactggtttg aggaacccta cgaagccttt gttgaactct ctgatctgta tgataagcat
     6301 attcacgatc agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg
     6361 gtggagctgg tggaaattaa ccatgttcat cgccgcgcag aatttcagat aattatctcc
     6421 ccggagtatc aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc
     6481 tttaccgttc tcaatctcta taagctgtat ctgatcgttg ataaagagaa tgaaaaagcg
     6541 attcacattt accgcaagct tggcttttcg gttgaaggtg aattgatgca cgagttcttt
     6601 attaatggtc aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca
     6661 gagcacaaaa caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa
     6721 tcgatcgtat ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca
     6781 atgattagct gacagtccac cggattagcg tgactgtcat attcacagct ctgttttaca
     6841 ttaccaaccc gttgattatt cagtaaagta accgctgtgt aatctaattt tttgatcgga
     6901 tccgttgatg gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc
     6961 agcggataac cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta
     7021 atcacgaagc cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc
     7081 tggcattttc cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg
     7141 gcgtccagta ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca
     7201 aaacagcctt cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc
     7261 attaatgtct gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta
     7321 gagaaactgg ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg
     7381 aggaatatcg cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag
     7441 agatgattct caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa
     7501 gaagttatct tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct
     7561 gctcgccctc gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag
     7621 cgatatatca cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat
     7681 tgtacccaat gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gtgctaatga
     7741 tacgcataaa attctctaca cccgcaccac cagcggtaac gtctctgctc ccgcgcagtc
     7801 tacccaggat ggcgcgcctg ccgaaccgca gtaatacatt atcgactgaa cgccggatac
     7861 gacacatccg gcgatttgaa catacaacat aatcccacct tattactcat acccttctat
     7921 tgatatggat taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct
     7981 aaatattcaa tatataaact tttatataac gataaagaac agggagtgag ttatgtccaa
     8041 aaatgaacga atggtgggca tcagccgcag aacactcgtt aaatctaccg cgataggttc
     8101 tctggcgctg gctgcaggcg gtttttcttt gccgtttacc ctgcgcagtg cagcagcagc
     8161 ggtacaacag gcccgcgaaa aagtggtctg gggtgcctgt tccgtcaact gtggtagccg
     8221 ctgtgcactt cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac
     8281 cggcagcgat gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg
     8341 ccggcgtatt aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg
     8401 tgaaggcaaa ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct
     8461 gaaaaaaacc gtcgaacaat atggcaatga ggctgtatat attcagtact cttcggggat
     8521 cgttggcggc aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa
     8581 ctgctacggc ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc
     8641 catgccctac acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa
     8701 gctggtcgtg atgtttggta acaacccggc agaaacccgc atgagcggcg gtggcatcac
     8761 ttatcttctt gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg
     8821 atataccgat acggctgctg gtcgtgaaga tgaatggctc cctattcgcc cgggcaccga
     8881 tgccgcgctg gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc
     8941 ttttctcgat aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa
     9001 aaatggtcac tataaagcct atattcttgg tgaaggtgat gataacacag cgaaaacgcc
     9061 gcagtgggct tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga
     9121 aattggcaca gcaaaacccg cttatatctg ccagggctgg gggccgcaac gccaggcaaa
     9181 cggcgaactg actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat
     9241 cagcggcgga aatagtggcg cgcgtgaatc gacctatacc attaccatag aacgcctgcc
     9301 ggtgctggat aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga
     9361 tcatggtccg caaatgacgg caatccgcga cggtgtccgg ggcaaagata aactggatgt
     9421 gcccattaag ttcatctgga actacgcggg aaataccctg gttaatcagc attctgacat
     9481 caacaaaacg catgaaattc tgcaagacga atcgaaatgc gaaatgattg tggtcatcga
     9541 aaactttatg acctcatcgg caaaatatgc cgacattctg ctgccagacc tgatgaccgt
     9601 tgagcaggaa gatattattc ctaacgacta cgccggtaac atgggatatc tcattttcct
     9661 ccagcctgtc accagcgaaa aattcgaacg caaaccgatt tactggatcc tgagtgaagt
     9721 cgcgaaacgt cttggacctg acgtctatca aaagttcaca gaaggtcgca cgcaggaaca
     9781 atggttacaa catctgtacg ccaaaatgct tgccaaagat ccggcgttac cgtcttacga
     9841 cgaactgaaa aaaatgggta tctataagcg taaagatccc aatggccatt ttgtcgccta
     9901 caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg gtaagattga
     9961 aatttattcc agcaggctgg cggaaattgc ccgtacatgg gaactggaaa aagatgaagt
    10021 gataagccca ttgccagttt atgcctcaac ctttgaaggc tggaactccc ctgaacgtag
    10081 aaccttccca ctgcaactgt ttggtttcca ttacaaatcc cgtactcact cgacctacgg
    10141 caacattgat gtcctgaaag cagcctgccg tcaggaggtg tggatcaacc ctatagatgc
    10201 gcagaaacgt gggattgcca acggcgatat ggtgcgggtg tttaaccatc gtggcgaagt
    10261 tcggctacca gccaaagtga caccacgtat tctccctgga gttagcgcta tgggccaggg
    10321 agcctggcac gaggccaata tgtctggcga taaaatcgac catggcggct gtgtgaatac
    10381 gctaaccact ctgcgccctt caccactggc gaagggaaac ccgcagcaca ctaatctggt
    10441 cgagatcgaa aaaatataac ccactacaac cataaattct ggcatgacat tttgtttgaa
    10501 aagcaataag tgagtaatga tgaaaatcca taccacagag gcgctcatga aggctgaaat
    10561 cagccgtaga agtctgatga aaacctccgc acttggcagt ctcgcgctgg caagcagtgc
    10621 tttcactctg ccattttccc aaatggtccg ggcggcagag gctccggtag aagagaaagc
    10681 ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt ctgttacgtt tgcatgtgaa
    10741 agatgacacc gtgtactggg tggagtctga tacgacaggt gacgacgtct acggtaatca
    10801 tcaggttcga gcgtgtttac gcgggcgctc tatccgccga cggatgaatc atcctgatag
    10861 gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg aacggataag
    10921 ttgggacgaa gccctggata ccatcagtga taatcttcgg cggatcctga aagattacgg
    10981 caatgaggct gtacatgtcc tgtacggaac aggcgtagat ggcggaaaca tcaccaactc
    11041 aaacgtcccg taccgtctga tgaactcttg cggtggtttt ctcagtcgct atggcagcta
    11101 cagtaccgca cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg atggcaacag
    11161 cccggatgat atcgccaata cgaaactggt cgttatgttc ggaaataacc cggcagaaac
    11221 gcggatgagc ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac gttcaaacgc
    11281 acgcatgatc gtcatcgatc cacgttataa cgacactgct gccgggcgtg aagatgaatg
    11341 gctgcccatt cgccctggca ccgatggcgc actggcctgt gcgattgcct gggtactgat
    11401 tactgaaaac atggtcgatc agccatttct cgacaaatat tgtgttggtt acgatgaaaa
    11461 aacgctgccc gccaacgcac cacgtaacgc gcattataaa gcctatattc tgggcgaagg
    11521 gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca tcccggcaga
    11581 aaaaattatc cagttggcgc gagagatcgg ttcagcaaaa cctgcttata tttgtcaggg
    11641 ttgggggcca caacgacatt ccaacggcga gcaaacgtcc cgcgctattg ccatgctttc
    11701 cgttctcacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg aaggtagctg
    11761 ggatctgggg gtagaatggt tcccgatgct tgagaatcct gttaaaacgc agatttccgt
    11821 ctttacatgg acagatgcca tcgaccatgg tacggaaatg accgcgaccc gtgatggtgt
    11881 tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg ccagtaacac
    11941 attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg atgacagcaa
    12001 gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt attgcgatat
    12061 cctgttgccc gacctgatgc caacagagca agaagacctt atctctcatg aatctgcagg
    12121 gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg aacgcaaacc
    12181 catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt atcaaacctt
    12241 tactgaaggt cgcagtcagc atgaatggat caaatatctc catgcgaaaa cgaaggaacg
    12301 taaccctgag atgcccgact acgaggagat gaaaacgacc gggatcttta agaaaaaatg
    12361 cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg caaacccgtt
    12421 gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga ttgcagatac
    12481 ctgggaattg aaaaaagatg aaattattca tccccttcct gcgtataccc caggttttga
    12541 tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct tccattacaa
    12601 agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct gcccacaaga
    12661 ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg ataccgtgcg
    12721 ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc gtattctgcc
    12781 tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg gtgaccgggt
    12841 cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc tggcaaaggg
    12901 aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag cccatgacca
    12961 cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc tgcgaactgg
    13021 cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt tatgaatacg
    13081 ctggcggcga ctggcaggaa gataatggcg tctggcacca gaacgtgttc gcttattatc
    13141 tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc cccagcggcg
    13201 caatgcataa gcgtgaagat ggctttgtgg tggttgatga agatgtttgt atcggctgcc
    13261 gctactgcca catggcttgt ccgtacggcg caccacagta caatgctgaa aaagggcaca
    13321 tgacgaagtg cgatggttgt tattcgcgcg tcgccgaggg gaaacaaccc atatgtgtcg
    13381 aatcctgccc gctgcgtgca cttgagttcg gtccaattga agagctgcgc cagaagcacg
    13441 gcactctggc tgccgtcgcg ccactgccgc gcgcgcattt cacaaaaccc aatatcgtta
    13501 tcaaacctaa cgccaacagc cgcccgaccg gcgataccac tggttatctg gctaatccgg
    13561 aggaagtgta aaatgggaaa tggatggcat gagtggccac tggttatctt taccgtattg
    13621 ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg gctggttcgc cgccaaaaat
    13681 gacgcagacc gtcagcgtat tgtccgtggc atgtttttcc tctggctgtt gatgggcatc
    13741 gggtttattg cctctgtcat gcacctggga tcgccactgc gagcgtttaa ctcgctgaat
    13801 cgaatcggag cttctggtct gagtaacgag attgctgccg gttccatatt ttttgccgtt
    13861 ggcggactat ggtggctggt ggcggtcatc ggtaaaatgc cgcaagcgtt ggggaaactc
    13921 tggttacttg tcagcatggc gctcggcgtc attttcgtct ggatgatgac ctgcgtgtat
    13981 caaatcgaca ccgtgccaac ctggcataac ggttacacta cgctggcttt cttcctgacg
    14041 gtattgttga gcggtccaat acttgctgcc gcaattctgc gggcagctcg cgttacattt
    14101 aataccacac catttgccat cattagcgtt ttggcgctga tcgcttgtgc cggggtgatt
    14161 gtgctgcaag gtttgtctct ggcgtctatt cactcatccg tgcaacaagc cagcgcactg
    14221 gtaccagatt acgcctcctt acaggtctgg cgtgtggtat tgttatgcgc aggtcttgga
    14281 tgctggcttt gtccgctcat tcgtcgccgt gaacctcacg ttgccggact tattctgggg
    14341 ctaatcctca tacttggcgg tgagatgatc gggcgtgtgc tcttttatgg cctgcacatg
    14401 acagtaggaa tggctattgc aggttaacaa attggtgcgg ggcaacccgc acatttcagg
    14461 atgttaggaa tgacccattt ttcacagcaa gataattttt ctgtcgcggc gcgcgtgttg
    14521 ggcgcgctgt tttattacgc tccagagagt gcggaagccg cacctctggt tgcggtactc
    14581 accagtgacg gctgggaaac tcagtggcct ctaccagaag cgtcattagc gccgctggtg
    14641 acggcttttc agacacagtg tgaagagact cacgcccagg cctggcagcg tttgtttgtc
    14701 ggcccgtggg cactgccgtc tccgccgtgg ggggcggtct ggctggatcg cgaatctgtg
    14761 ctgtttggcg attcaacatt ggcacttcgt cagtggatgc gcgagaaagg cattcagttt
    14821 gaaatgaagc aaaacgaacc ggaagatcat tttggttcac tgctgttgat ggctgcgtgg
    14881 ttggcggaaa atggtcgcca gacagaatgc gaagaattgc tggcatggca cctttttccg
    14941 tggtcaacac gttttcttga tgtttttatc gaaaaagcag aacacccttt ctaccgtgca
    15001 ctgggtgaac tggctcgctt aacgctggcg caatggcaat cacaactgtt aattcctgtc
    15061 gcggttaaac cgctgtttcg ataggatcac cgtaatattg ccggatggtg acgattacgc
    15121 gcaatccggc aataataggt tacagtgtca cgttttttta tctcttaaag cacgcactgc
    15181 ttttgcggct ggcctctttt gtcgcaaaat agtcgcccgt gtttcattgc ccatttctgc
    15241 tcatgcatca tctacacatc tatccggatc tgcgcactat gttccgccgt ctgcttatcg
    15301 caacagtcgt cggtattctc gcggcctttg ccgttgccgg gtttcgtcat gcgatgctgc
    15361 tactggagtg gttgttcctc aataatgact ccggcagtct ggtcaatgca gcgacaaacc
    15421 tttccccctg gcgacggttg ctaactccgg cgctcggcgg actggcggcg ggtttgttgc
    15481 tgatgggctg gcagaaattt acccaacaac gccctcatgc gccgaccgat tacatggaag
    15541 cgttgcaaac cgatggacag ttcgattacg cagcaagcct ggttaaatcg cttgcctcgc
    15601 tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt cttttagctg
    15661 cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg ccaggagtgg aaattatgga
    15721 tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg tgccccgctt gctggcagtt
    15781 tatttatagc cgaagtgctg tttggcacta tgatgttggc ctctctcggc ccggtgatta
    15841 tttccgccgt cgtggcattg ctggttagca atctgattaa tcatagcgac gcgttactct
    15901 acaacgtaca actctcagtg acggttcagg ctcgtgacta tgcgctgatt atcagtacag
    15961 gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc tgtcatcgtg
    16021 gatttgtgag tctcaaactt gcgccgccct ggcaactggc actaggcggg ttgatcgtgg
    16081 gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc gtacaatcct
    16141 ttttaaccgc cccaccactg ttaatgatca ttgccgggat cttcctttgt aaactgtgtg
    16201 ccgtgctggc gagtagcggt tccggcgcac ccggtggggt ctttacaccg acgctattta
    16261 tcggtcttgc cattggcatg ttgtatggtc gtagcctggg attatggttc cctgatggcg
    16321 aagaaattac acttttactc ggattgaccg ggatggcgac actgttggcg gcgaccacgc
    16381 acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac cggggagtat cagctactcc
    16441 ccggtttatt gattgcctgc gtaattgcgt cggtaatttc gcggacgtta caccgtgact
    16501 ctatctaccg ccagcacact gcgcagcata gctaagcgga tgtattgccc cagttcacgc
    16561 tgttcggcgc gcggcagata aggcagttca ccaatgagcg gtgccggaag ttttttaccc
    16621 agcacatcaa tgatttccgc ataatgcgcc agtcctgggt tgattcggtt agccacccag
    16681 ccaatgagcg gcagcccgtc gttggcgatc gcctgagctg ttagcagtgc atggttaatg
    16741 caaccttcct gaataccgac aaccatcaac accggcagtt gttcctgcac tacccattca
    16801 gagagtggac gcaaatcatt catcagactg cgccagccgc cagtcccttc taccacgaca
    16861 tgatcgactt tttcggtcag gtttgccagg ccgtttgaaa tgagggtgta attgattggg
    16921 caactgtgcg ccacgctact ttcttcttcg cttaacgcga taggattaac tgcttcataa
    16981 ggcagttcga tggttgaaac actctgcaac accagggcat ctttattacg cagcccttcg
    17041 ggtgtctctt tgctcccctt cgctacgggt ttatatcccg caaccgtttt tccctgggag
    17101 gctaacgctt gtagcaatgc gcgggaaacc accgttttcc ctacagaagt gtctgtaccg
    17161 gtaataaaga aacgcttcag catcactaac tccaccgtta tgcttcacaa atataaacca
    17221 ggaaaataat taaccttgaa agtctaagtt atgctttcct ggcccaaatt gagatagcgc
    17281 aaattttggt agaacagtta aaaaatgtta accctgcaac agacgaatca acaaagaacc
    17341 gttatacatc gcgtctttta ccagtgcagc gcctgccatc gtgccctggt tagaaaactg
    17401 agtactctca acgctgatgg gctgactata cgcaggaagg gcctgctgac ggatgctgtc
    17461 tgagatgacc gggaagagga tatctgccgc tttacttaac ggtgagccaa tcagtatttt
    17521 ttgtgggtta aataaattca ccatgatggc aagaatgcgc cctacatgcg cgcccacccc
    17581 ggtaatgatg tcttttgcca gcagatcgcc gcgcaatgcc gcctgacaca atgagtccac
    17641 ggttaacggt tgtccatgta acatcgagct catggattga ttaagacgca gctgtgccag
    17701 ctcaagtata ctgtccacac tggcgatggt ttcgaggcag ccgtgattcc cgcaataaca
    17761 acgtttccca tacgggtcga cctgtgtgtg gcctatttcc acgaggctac tgctgcctgc
    17821 gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg ttgtgatcga taaccacctg
    17881 aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc tctgccatcg tccatgcgct
    17941 gatatcatgc tgaatataaa ccggaacgcc ggtatgctgc tccagagcct cgccgagcgg
    18001 catctctttt acatcctcgt agaacggcat gcgatgtaca atgccatttt ccgtatcaat
    18061 aattcccggc agggttatgg caatcgaagt tagacgctca agttttttct ggtggcggat
    18121 aaaaaactga tcgatatggg aaatgatacg atccagcaat ggcgagtcat cttttaacgc
    18181 cagttcctgc gactcttcca ccaccagttt gctgctcaga tcgcgcagag caaggaaaat
    18241 ctccccgcga ctaatgcgca gagaaagata gtgccaggct tcagtttcaa ccaccagccc
    18301 caccgccgga cggccacggt tccccgcttc tttgatttcc agctcttgca ccaggtgtgc
    18361 ttcgagcatc tcacggacaa ttttagtgat actggcagga gccagttgcg ccagacggga
    18421 aagatcgata cgcgagactg gaccaagctg atcaatcagg cgataaaccg cgcccgcgtt
    18481 ggtctgcttt atttgatcaa tgtgcccagg ctggttttca gcaaccaccg catactccct
    18541 atattttcgc gctccgaaat aatctgtagg ctatggtgaa gcacttcaat acgtgtcgtc
    18601 aaatttttac ttaggcatgt gattaacagc acatttttcg ggctttttcg ctgaaatttc
    18661 cctcacctga gagcattcag cagatgaata cgaaagttac gcgcagctgg actttgttca
    18721 tgatgtttcg gccagaccaa ccacatttca gaaaccgcat cctcttcagc aatcggcacc
    18781 cagcgcattt cgttgagctg aacacgttta aatgaagcag gcaaaattga aacacccaga
    18841 ccggcggaaa ccagaccgat gatggtcatt gcctcgccca cctcctgagt gatgacgggc
    18901 gtcaaatggt aacgtcgcat cagtccgaga atatcgtcat acagccctgt cccgacgtgc
    18961 ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca gcgttacatt cgggttattt
    19021 gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt catggacgat gactgcgtgt
    19081 tcaagcgtct ccggtaacgc tgtgttacgc agcaatccca tatccagcgt tccttcaatg
    19141 agcggagcga tttgctcacg agtgttcatt tcgcgggttt gtaaatggac atcaggataa
    19201 tcacggcgaa acagcgataa cgtatcggac accgcccgaa taaaaggagc ggacgaagta
    19261 aaaccaatac gcaactcccc cgcttcaccc tgatgcagcc tttcagcgcg agcggcagcg
    19321 tcatccacca tagacaggat ttgccgacta tctgcaagaa actgttttcc tgctgccgtc
    19381 agcaatacac tgcgattggt tcgtgccagc agtcgggcac caatttgttg ctccagcgcc
    19441 tgaatctgct gacttagcgg cggttgcgaa atattcaggc gggcagcggc gcgcccgaaa
    19501 tgcagctctt ccgcaacagc aacaaa
//
LOCUS       DAZFFI010000083        19434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFFI010000083 DAZFFI010000000
VERSION     DAZFFI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.083"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..1712
                     /gene="mqo"
                     /locus_tag="ACHJ12_003476"
     CDS             66..1712
                     /gene="mqo"
                     /locus_tag="ACHJ12_003476"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4991212.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQFKPQPVQKEVADIAL"
     gene            complement(1861..2370)
                     /gene="eco"
                     /locus_tag="ACHJ12_003477"
     CDS             complement(1861..2370)
                     /gene="eco"
                     /locus_tag="ACHJ12_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4991213.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2484..2648)
                     /locus_tag="ACHJ12_003478"
     CDS             complement(2484..2648)
                     /locus_tag="ACHJ12_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991214.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNCYKHIYIAINSPEI
                     FLLVCSLTRI"
     gene            2757..3251
                     /gene="napF"
                     /locus_tag="ACHJ12_003479"
     CDS             2757..3251
                     /gene="napF"
                     /locus_tag="ACHJ12_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4991215.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAMIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3241..3504
                     /gene="napD"
                     /locus_tag="ACHJ12_003480"
     CDS             3241..3504
                     /gene="napD"
                     /locus_tag="ACHJ12_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4991216.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3501..5987
                     /gene="napA"
                     /locus_tag="ACHJ12_003481"
     CDS             3501..5987
                     /gene="napA"
                     /locus_tag="ACHJ12_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO4991217.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            5994..6689
                     /gene="napG"
                     /locus_tag="ACHJ12_003482"
     CDS             5994..6689
                     /gene="napG"
                     /locus_tag="ACHJ12_003482"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4991218.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6676..7539
                     /gene="napH"
                     /locus_tag="ACHJ12_003483"
     CDS             6676..7539
                     /gene="napH"
                     /locus_tag="ACHJ12_003483"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4991219.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7536..7985
                     /gene="napB"
                     /locus_tag="ACHJ12_003484"
     CDS             7536..7985
                     /gene="napB"
                     /locus_tag="ACHJ12_003484"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4991220.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            7995..8597
                     /gene="napC"
                     /locus_tag="ACHJ12_003485"
     CDS             7995..8597
                     /gene="napC"
                     /locus_tag="ACHJ12_003485"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4991221.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8610..9233
                     /gene="ccmA"
                     /locus_tag="ACHJ12_003486"
     CDS             8610..9233
                     /gene="ccmA"
                     /locus_tag="ACHJ12_003486"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4991222.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9230..9892
                     /gene="ccmB"
                     /locus_tag="ACHJ12_003487"
     CDS             9230..9892
                     /gene="ccmB"
                     /locus_tag="ACHJ12_003487"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4991223.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9934..10671
                     /gene="ccmC"
                     /locus_tag="ACHJ12_003488"
     CDS             9934..10671
                     /gene="ccmC"
                     /locus_tag="ACHJ12_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4991224.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10668..10877
                     /gene="ccmD"
                     /locus_tag="ACHJ12_003489"
     CDS             10668..10877
                     /gene="ccmD"
                     /locus_tag="ACHJ12_003489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4991225.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10874..11353
                     /gene="ccmE"
                     /locus_tag="ACHJ12_003490"
     CDS             10874..11353
                     /gene="ccmE"
                     /locus_tag="ACHJ12_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4991226.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11350..13293
                     /gene="ccmF"
                     /locus_tag="ACHJ12_003491"
     CDS             11350..13293
                     /gene="ccmF"
                     /locus_tag="ACHJ12_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4991227.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13290..13847
                     /gene="dsbE"
                     /locus_tag="ACHJ12_003492"
     CDS             13290..13847
                     /gene="dsbE"
                     /locus_tag="ACHJ12_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4991228.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13844..14896
                     /gene="ccmH"
                     /locus_tag="ACHJ12_003493"
     CDS             13844..14896
                     /gene="ccmH"
                     /locus_tag="ACHJ12_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4991229.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSLQESK"
     gene            complement(14931..15578)
                     /gene="narP"
                     /locus_tag="ACHJ12_003494"
     CDS             complement(14931..15578)
                     /gene="narP"
                     /locus_tag="ACHJ12_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4991230.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15898..18489
                     /gene="yejO"
                     /locus_tag="ACHJ12_003495"
                     /pseudo
     CDS             15898..18489
                     /gene="yejO"
                     /locus_tag="ACHJ12_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(18981..19262)
                     /locus_tag="ACHJ12_003496"
     CDS             complement(18981..19262)
                     /locus_tag="ACHJ12_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991231.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSMTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
BASE COUNT         4415 a         4766 c         5535 g         4718 t
ORIGIN      
        1 tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
       61 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
      121 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
      181 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
      241 tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
      301 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
      361 gcatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
      421 gggcgcacca ggttgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
      481 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
      541 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
      601 aagagtgggc accgttagtg atggaagggc gcgatccaca acagaaagta gcggcaacac
      661 gcacagaaat tggcactgat gtgaactatg gcgagatcac ccgccagtta attgcttcct
      721 tacagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
      781 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaacggcact gcacagaaca
      841 tccgcgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagcta ttacaggaat
      901 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
      961 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
     1021 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
     1081 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
     1141 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
     1201 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
     1261 aagcgttgaa agagtactat ccgcaggcaa aaaaagagga ctggcgtttg tggcaagcgg
     1321 ggcagcgcgt gcagattatc aagcgtgatg ccgagaaagg tggcgtactg cgtctgggta
     1381 ctgaagtggt cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg
     1441 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
     1501 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
     1561 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
     1621 aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttcaaa ccgcaacccg
     1681 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
     1741 ggatgtggcg tatgctgata agacgcgcca gcgtcgcatc aggcaccggc tcgggcgtta
     1801 gatgtcggat gcggcgtgac cgccttattc ggcctacggg gtgccgcact tcacagcatt
     1861 ttagcgaact accgcgttgt caattttctc ttccgccttc cagacgcggt acttcacatc
     1921 tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca gcattccagc
     1981 atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc aggccatcat
     2041 cgtggaaacc ggcgagctaa ctttatcaaa gacataataa tcatagcccc agccttccag
     2101 cgttttgctt tccagcttcc cgccgagacg atgcaaattg caatcgactt ccagcgtctg
     2161 accgattaac agttctactt tcagggtaga ttcatcttct tgcggggtta actgaatcac
     2221 ctgacgcttc atcccttttt cagcttgtgg ataaggcgcg attttttcca gtggctggac
     2281 gctttctgcc gcccaggcgg aagtggtagc gaaagcggca aacaatactg caggtagaat
     2341 ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga tgtcgctgaa
     2401 ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag aacattcaga
     2461 caattttacc tgctctttta gtgttaaatt ctggtgaggg agcaaaccag cagaaatatc
     2521 tctggtgaat taatcgctat ataaatatgt ttataacaat ttgaaatgtg agcaaaagcc
     2581 cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg
     2641 gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta
     2701 tgcggtttat ttggccgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga
     2761 agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta
     2821 tccgtccgcc ttggtccggt gatgaatctc attttctgac ccattgcacc cgttgcgacg
     2881 cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg
     2941 ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat
     3001 cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt ggggacgcgt
     3061 gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa
     3121 tgatctttcg cccgacgctc tccgggatct accagccgca acttaatagc caactttgca
     3181 acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc
     3241 atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca
     3301 gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg
     3361 agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt
     3421 gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa
     3481 gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg
     3541 ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg
     3601 gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg
     3661 gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac
     3721 cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta
     3781 aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg
     3841 aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg
     3901 ccctgaaaga aaaaggaccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct
     3961 gggaaggtta tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac aacatcgacc
     4021 cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg
     4081 atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg
     4141 ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaaccgt cgtctctcta
     4201 accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg
     4261 ataacggcat catctttacg ccacagtctg acctggtgat cctgaactac atcgccaact
     4321 atatcattca aaacaatgcg ataaatcagg acttcttcag caagcacgtt aacctgcgca
     4381 aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga
     4441 agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg
     4501 ccgagtatac gctggaaaaa actgccgaaa tgaccggcgt gccgaaagac cagttagaac
     4561 aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct
     4621 tcaaccagca tactcgtggc gtgtgggcca acaacctggt ctacaacctg cacctgctga
     4681 ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttccgcgt
     4741 gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga
     4801 ctaacgagaa acaccgtgat atttgcgaga agaagtggaa tatcccgagc ggcaccattc
     4861 cggcgaaaat cggtctgcac gcggtggcac aagaccgtgc actgaaagac ggcaagctga
     4921 atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatt aatgaagagc
     4981 gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag
     5041 tcagtgcgct ggcagccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggcg
     5101 cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag gcgccaggcg
     5161 aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag
     5221 tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag
     5281 ttctgtatgc cacccccgaa gtgagcaaat tcccggtatc cgaactggcg gaagatcagc
     5341 tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg
     5401 catggtttgg tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc
     5461 gcggtctgcg ctggccggtg gtgaacggta aagaaacaca gtggcgttac agcgaaggta
     5521 acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg gatggcaaag
     5581 cggtgatctt cgcactgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc
     5641 tgtggctctc taccggacgc gttctggagc actggcacac cggcagtatg actcgccgtg
     5701 tgccggaact gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg ctggatgcga
     5761 aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga
     5821 tctcgattgt tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt
     5881 tcttcgacgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat ccgctctcga
     5941 aagagacgga cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc
     6001 ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt cgcacagcag
     6061 gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg
     6121 gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc
     6181 gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggcct
     6241 ctggtctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg
     6301 aggacattcc gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag attgaatcga
     6361 tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc
     6421 aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc
     6481 tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca
     6541 gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa caaccggcaa
     6601 tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggtt
     6661 ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa
     6721 aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg
     6781 ctggggatgt ttttgagcgg tccatggttt ggtgtgtgga tcttgcacgg caactacagc
     6841 agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt
     6901 gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat caccgtgctc
     6961 tacgccctgg cgggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc
     7021 gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc
     7081 cacattcgct acgtgctgtt ggtggtcatt ctgctgggat cggccttaac tggcacgctc
     7141 atctgggaat ggatcaaccc cgtatctctg atggggcgca gcctggtaat gggcttcggc
     7201 agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc
     7261 tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg
     7321 attaccgttg cggccaccga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt
     7381 tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc
     7441 accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt
     7501 acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag
     7561 cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta
     7621 atggcgtcga ttttagccag tcgccggaag tatccgggac acaggaaggg gccattcgga
     7681 tgccaaaaga gcaggaacgg atgccgctga actatgtgaa tcagccgcca atgatcccgc
     7741 atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg
     7801 tcgaaagtta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg
     7861 acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac
     7921 cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga
     7981 aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga
     8041 aatggtggcg tacccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgttg
     8101 gcggcatcgt cttctggggt ggctttaaca ccgggatgga aaaagccaat accgaagagt
     8161 tctgcattag ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg gattccgtgc
     8221 actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt
     8281 ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg
     8341 gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca cagaatgagt
     8401 ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata
     8461 tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg
     8521 ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag
     8581 tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt
     8641 gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt
     8701 gggtacaaat caccggtagc aacggtgcgg ggaagacaac gcttctccgt ttgctgacgg
     8761 ggttgtctcg ccctgacgca ggcgaggttc tctggcaagg gcagcccttg catcaggtac
     8821 gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc
     8881 tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg
     8941 aagccctggc gcaggccggg cttgccggat tcgaagatat tcctgtaaat cagctctcgg
     9001 ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga
     9061 tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta
     9121 tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg
     9181 ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca tgatgttctg
     9241 gcgcattttc cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc
     9301 gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca
     9361 actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct
     9421 ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt
     9481 gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac
     9541 cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa tggatgttta
     9601 tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg gctttctcgg
     9661 tgcaccgggc gtggcgctga cagtgggact taagcgcggt ggtgtgctgc tcagcatact
     9721 ggtgttaccg ctgactatcc cattactcat cttcgccacc gccgcgatgg acgcggcttc
     9781 catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc
     9841 gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat
     9901 tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg
     9961 atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt
    10021 gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag
    10081 cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc
    10141 atttatgcat caatggcagt ggcagcgttt attggccttg tctggcagat gaaaatggcc
    10201 aacctggcgg tggcggcgat ggcccccatt ggtgcagtgt ttacctttat tgccctggtt
    10261 accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg
    10321 acttctgaac tggtgctgct gtttttgtat gtgggtgtga tcgccctgtg gcacgccttc
    10381 gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat
    10441 ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcga
    10501 atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc
    10561 ctgctgctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa
    10621 aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat
    10681 ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg
    10741 tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg cagcatcgcg
    10801 caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac
    10861 agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt
    10921 ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct
    10981 cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt
    11041 cgggcagcgt ctgcgcgtcg gcgggatggt gatgccgggt agcgtgcagc gcgatcccaa
    11101 ttcgctaaaa gtaactttca ccatttacga cgctgaaggt tcagtagatg tctcttacga
    11161 aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgaactgga
    11221 aaaaggcaat catatcctcg caaaagaagt gctggcgaaa cacgacgaaa actacacgcc
    11281 gccagaagtt gagaaagcga tggaagctaa tcaccgtcgc ccggcgagtg tttataagga
    11341 cccagcatca tgatgccgga aattggtaac gggctgctgt gtctggcgct aggaattgcg
    11401 ctgctgctgt ccgtgtatcc gctatggggc gtagcgcgcg gagatgcgcg catgatggcg
    11461 tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtactg
    11521 gtcaatgcct tcgtggtcaa cgacttcacc gtcacctatg ttgccagcaa ctccaatacc
    11581 cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctcctg
    11641 ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt
    11701 ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg
    11761 ctgttcattc tctttacctc taacccgttc tcacgcacgt tgccgaactt cccgattgaa
    11821 gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc gcctctgctc
    11881 tatatggggt acgtggggtt ctcggtggcg tttgcttttg ccattgcttc tttgttgagc
    11941 gggcgtctgg acagcactta tgcgcgtttt actcgtccgt ggacgctggc ggcgtggatc
    12001 ttcttgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc
    12061 ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact
    12121 gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta
    12181 ctgctggcaa tcagtgcctt ctcgttgtgt ctgctgggga ctttcctggt gcgttccggc
    12241 gtgctggtat cggtacacgc gtttgcctct gatccggcac gcggtatgtt tatcctcgcc
    12301 tttatggtac tggtgattgg tggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt
    12361 cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt
    12421 ttgctggtcg ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag
    12481 caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg
    12541 ctgatggtgc cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat
    12601 cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg
    12661 ctgctgctgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggcctggca
    12721 atggcctgct ggattgctgt gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc
    12781 acgaaaacca ccttcagtta ttggggaatg gtggcggctc accttgggct ggcagtgaca
    12841 attgttggca ttgcctttag tcagaactat agcgttgagc gtgatgtgcg catgaagtcc
    12901 ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact
    12961 ggcccaaact ggcgcggcgg tgtggcgact attggcgtaa cgcgagaggg taaaccggaa
    13021 acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg
    13081 gcaattgacg gcggcatcac acgtgacctg tacgccgcgc tcggtgaaga gctggaaaac
    13141 ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg
    13201 ctgatgatgg cgttgggcgg actactgtgt ctgtttgatc ctcgctatcg taagcgcgtg
    13261 agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta attccgttga
    13321 ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg
    13381 atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag tttcgtctcg
    13441 aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag ggcaaaccag
    13501 tactgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga
    13561 atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac gatcgccaga
    13621 aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg
    13681 acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaacgttc cttattgacg
    13741 gcaacggcat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag
    13801 agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg
    13861 cgtgctgatg ctgatgatct ccggctcagc gctggcgacc atcgacgtgt tgcagtttaa
    13921 agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg
    13981 ccagaataac agcattgccg attccaactc gatgattgcc accgacctgc gccagaaagt
    14041 gtatgaactg atgcaggaag gtaaaagtaa gaaagagatt gtcgattata tggtggcgcg
    14101 ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt
    14161 gctgccagta gtggctattg gcattggcgg ttgggtcatt tacgcccgct cgcggcgtcg
    14221 ggtacgcgtg gtgccggaag cgtttcctga acaaagcgtg ccggaaggta agcgtgccgg
    14281 atatgtcgct tatttgccgg gcattgtggt ggcgttaatt gtggctggcg tcagctacta
    14341 ccagaccgga aattatcaac aggtgaaaat ctggcagcag gccacggcac aggctccggc
    14401 gttgctggac agggcgctgg atccgaaagc cgatccgctc aacgaagaag agatgtcgcg
    14461 ccttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag acggctggat
    14521 tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg atgcatacgc
    14581 tactgcgtat cgcctcgatc cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt
    14641 gacacgttca tctgatccca acgacaaccg cctcggcggc gaactgctgc gccagttggt
    14701 gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg cctttgagca
    14761 gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa
    14821 cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgct
    14881 gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc
    14941 ccgcgttgtt gcaggaacag aatggtggcc gccacgcgtg agcggacatt gagtttacgc
    15001 agcagattgc gaatatgtac ttttactgtc tgctcggaaa tattcaatac cgaggcaatc
    15061 tgtttatttg acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc
    15121 acgctgaagg gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg
    15181 cgttcgctaa agactttgct gcctttcgct ccggcacgaa tcgcttccag caatacttcc
    15241 gggtcgctgt ctttcaacag ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg
    15301 ctggaggcat cggatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag
    15361 gcattgaggg tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg
    15421 atatccagtc tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact
    15481 tcaaagccag gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca
    15541 tccacaatca tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt
    15601 agaatcatta tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca
    15661 ttttatgtat ggacatgtag tgaaaactac aaccttgtcg ttcgctttgg catcattttc
    15721 attataaaga cggaattttg catcagcttc tcatcaagaa aaacaactaa aaaatcaatc
    15781 acaatacttg tgcatttctt taaacagttt ttaaaaactg actccgggta tggagctatg
    15841 ggtattttcc gtacccaatg cttttaacag caattaattt cataggatga aagctcaatt
    15901 catcaatctg gttctgtttc tctttgtcgt tccgcaatat ctgttctggt ggctacagcg
    15961 ttatattcac ccatagcatt ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt
    16021 gtcctggaaa aagatatcca gctggtttat gggaccgcca ataatacgaa aatcaatcct
    16081 ggcggagaac agcatataaa agaatttggt gtaagtagta atactgaaat taaaggaggg
    16141 tatcagtaca ttgaaatgaa tggtaccgca gaatactcag tattaaatga cggttatcaa
    16201 attgttcaaa tgggtggcgc ggcaaaccag actaccctca ataatggtgt gctacaggtt
    16261 tatggcgcag cgaatgatcc cacgattaaa ggcgggcgct taatcgttga aaaagatggg
    16321 attaccgtcc ttgccgctat cgaaaaggga ggattactgg aggttaaaga ggggggatta
    16381 gcgattgcgg tagatcagaa agcaggcggt gctattaaag caagcacgcg ggtcatggag
    16441 gcattcggaa caaaccgtct cggtcagttc gaaatcaaga atggtattgc taacaatatg
    16501 ctgttggaaa acggcggaag tttgcgagtt gaagaaaatg acttcgctta taatactact
    16561 gtagatagtg gcggcttact ggaggttatg gatggcggga ctgtaactgg cgttgataaa
    16621 aaagcaggcg gaaaattaat tgtctcaacg aatgcgctgg aagtgagtgg tacaaacagt
    16681 aaaggccaat ttagtataaa agatggtgtg tcaaaaaatt atgaactgga tgatggttcc
    16741 gggctcattg ttatggagga cacgcaggcc attgatacta tccttgatga gcatgccact
    16801 atgcaatcgc tgggaaagga tactggtacg aaagtgcagg caaatgcggt atatgatctc
    16861 ggtcgatcag atcagaatgg aagtatcacg tattcctcaa aagccatttc tgaaaatatg
    16921 gttatcaaca atggccgcgc taacgtctgg gctggcacaa tggttaacgt ttcagtcaga
    16981 gggaatgatg gcattcttga ggtcatgaag ccgcaaataa attatgcacc cgcaatgttg
    17041 gtgggtaagg tagtggtttc tgagggcgct tcttttagaa cgcatggtgc cgtggatacc
    17101 agcaaagcgg acgtttcgct cgaaaatagc gtatggacca tcattgccga tatcactacg
    17161 acgaaccaaa acaccctcct caacttagcc aaccttgcga tgtctgacgc aaatgtgatt
    17221 atgatggatg agccagtgac tcgttcatca gtgacggcaa gtgcggaaaa tttcattacg
    17281 ttgaccacca ataccctgtc gggaaacggc aatttttata tgcgtaccga tatggctaat
    17341 catcagagcg atcagctcaa cgtcaccggt caggcaacag gtgatttcaa aatattcgtg
    17401 acggacaccg gtgccagccc ggcagcagga gatagcctta cactggtaac aacgggcggc
    17461 ggtgatgctg catttacgtt gggcaatgcc ggaggcgttg ttgatatcgg tacgtatgaa
    17521 tataccttgc tggataatgg caaccatagc tggagtttgg cagagaatcg cgcgcaaatt
    17581 accccttcaa ccactgatgt gctgaatatg gcggcagcac aaccgctggt atttgatgca
    17641 gaactggaca ccgtgcgtga gcgtcttggt agcgtaaaag gcgttaatta cgatacggcg
    17701 atgtggagtt cggcaattaa cacccgcaac aacgtgacca ctgatgcggg cgctggcttt
    17761 gagcaaacat tgaccgggct gacgctcggt atcgatagcc gtttctcccg tgaagaaacc
    17821 agcacaattc gcggcttgtt ctttggttac tctcattctg atattggttt tgatcgcggc
    17881 ggcaaaggca atgtcgatag ctataccctg ggggcttatg ccggttggga gcatcagaac
    17941 ggtgcctatg ttgatggagt ggtgaaagtt gaccgttttg ccaacaccat ccattgcaag
    18001 atgagtaatg gggcaacagc gtttggcgat tacaatagta acggcgcggg tgctcatgtc
    18061 gagagcgggt tccgttgggt tgacggattg tggagtgtta gaccctatct ggcctttacc
    18121 ggctttacca cagatggtca ggactacacg ttatcaaacg gcatgcgcgc ggatgtggga
    18181 aatacccgga tattacgcgc tgaagcggga acggcggtaa gctatcacat ggacctgcaa
    18241 aacggtacga cgctggaacc ctgactgaaa gccgccgtgc gtcaggaata cgccgattct
    18301 aaccaggtga aagttaatga cgatggcaaa tttaataatg atgtggctgg aacccgtggc
    18361 gtttatcagg ctgggataag gtcatcgttt accccgacgt taagcggtca tttgtcagtc
    18421 agctatggca atggcgcagg ggtagaatcg ccgtggaata ctcaggcggg tgtggtctgg
    18481 acgttctgat aacaaaaaat aaacaggctg tgatgtgtca cggtctgttt atcgaattaa
    18541 ttgcagatat aaaaaaaacc aaccgtaaag gttgattttt tcttgggatt tttggtcgca
    18601 cgagagtagt ttaattgagt gtgtaactac atgattttaa ataatatgct tgattcaact
    18661 tttaaatcgt ataaccaaac gtataactaa atttatttgc tgaaaaagcg tacaatttaa
    18721 aggcgagttt tcccagaggt ttattaaact gcgaaaattt cgtagttatc cgttatcatt
    18781 ttccggctat ccgttatgaa gctggtggat gttccccgat atggggatcc ccatatcgag
    18841 aaccctaccg gatttttttt ccggttaaca gttaatcagg ctggtgggag gggtattaaa
    18901 tactccccct gtttaaatac cgtctttttg tcacgtcgtg tcactggttg tcactctccg
    18961 tcacggtatg tcacaactcg ttaacgccgt aaccgctccg gctttttcca gtggtacgta
    19021 attttttctt tctcccgata cagttcaacg cggcgattgt aggccagcat ttccagaacg
    19081 cggatccgta tgtcgcgcat atccgcatca ttaagctgga taccatcacg gcgcatcacc
    19141 tcagcaacaa cacgcgcata attttcggcg gtcatgctgt ccggctgcgt ggcctgttcg
    19201 tcagcctgct ggctgattcc ggcgacgcgg cggattaatc ccagtatttc ggcttctgtc
    19261 attgtgcccc catcgcgctg gtgaagaaaa caagctcaga ttttttgtaa agaatctgtc
    19321 acgctaaaag atgtcgaaca aaaattaacc acaatcatca tcttttttga atcaaccaca
    19381 ttaaaaacaa caggttacac acatgatgat gacgataaaa tcacaaaaat gcgc
//
LOCUS       DAZFFI010000084        19368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFFI010000084 DAZFFI010000000
VERSION     DAZFFI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.084"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..535
                     /locus_tag="ACHJ12_003497"
     CDS             230..535
                     /locus_tag="ACHJ12_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO4991232.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            642..1286
                     /gene="gfcB"
                     /locus_tag="ACHJ12_003498"
     CDS             642..1286
                     /gene="gfcB"
                     /locus_tag="ACHJ12_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4991233.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1283..2029
                     /locus_tag="ACHJ12_003499"
     CDS             1283..2029
                     /locus_tag="ACHJ12_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4991234.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVALVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2029..4125
                     /locus_tag="ACHJ12_003500"
     CDS             2029..4125
                     /locus_tag="ACHJ12_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4991235.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4171..5310
                     /locus_tag="ACHJ12_003501"
     CDS             4171..5310
                     /locus_tag="ACHJ12_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4991236.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5298..5744
                     /gene="etp"
                     /locus_tag="ACHJ12_003502"
                     /gene_synonym="yccY"
     CDS             5298..5744
                     /gene="etp"
                     /locus_tag="ACHJ12_003502"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4991237.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5764..7944
                     /gene="etk"
                     /locus_tag="ACHJ12_003503"
     CDS             5764..7944
                     /gene="etk"
                     /locus_tag="ACHJ12_003503"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4991238.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8059..9357)
                     /gene="appA"
                     /locus_tag="ACHJ12_003504"
     CDS             complement(8059..9357)
                     /gene="appA"
                     /locus_tag="ACHJ12_003504"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4991239.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9437..9529)
                     /gene="cbdX"
                     /locus_tag="ACHJ12_003505"
     CDS             complement(9437..9529)
                     /gene="cbdX"
                     /locus_tag="ACHJ12_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4991240.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9542..10678)
                     /gene="appB"
                     /locus_tag="ACHJ12_003506"
     CDS             complement(9542..10678)
                     /gene="appB"
                     /locus_tag="ACHJ12_003506"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4991241.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10690..12234)
                     /gene="appC"
                     /locus_tag="ACHJ12_003507"
     CDS             complement(10690..12234)
                     /gene="appC"
                     /locus_tag="ACHJ12_003507"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4991242.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12368..13225)
                     /locus_tag="ACHJ12_003508"
     CDS             complement(12368..13225)
                     /locus_tag="ACHJ12_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4991243.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNSLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEISPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13222..13620)
                     /gene="hyaE"
                     /locus_tag="ACHJ12_003509"
     CDS             complement(13222..13620)
                     /gene="hyaE"
                     /locus_tag="ACHJ12_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4991244.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVESQQERAS"
     gene            complement(13617..14204)
                     /gene="hyaD"
                     /locus_tag="ACHJ12_003510"
     CDS             complement(13617..14204)
                     /gene="hyaD"
                     /locus_tag="ACHJ12_003510"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4991245.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14201..14908)
                     /gene="hyaC"
                     /locus_tag="ACHJ12_003511"
     CDS             complement(14201..14908)
                     /gene="hyaC"
                     /locus_tag="ACHJ12_003511"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4991246.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14927..16720)
                     /gene="hyaB"
                     /locus_tag="ACHJ12_003512"
     CDS             complement(14927..16720)
                     /gene="hyaB"
                     /locus_tag="ACHJ12_003512"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4991247.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NSVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16717..17835)
                     /gene="hyaA"
                     /locus_tag="ACHJ12_003513"
     CDS             complement(16717..17835)
                     /gene="hyaA"
                     /locus_tag="ACHJ12_003513"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4991248.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18262..18349
                     /locus_tag="ACHJ12_003514"
     tRNA            18262..18349
                     /locus_tag="ACHJ12_003514"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18296..18298,aa:Ser,seq:tga)
     gene            18490..18819
                     /locus_tag="ACHJ12_003515"
     CDS             18490..18819
                     /locus_tag="ACHJ12_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4991249.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(18983..>19368)
                     /locus_tag="ACHJ12_003516"
     CDS             complement(18983..>19368)
                     /locus_tag="ACHJ12_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO4991250.1"
                     /translation="LLTAEKARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQT
                     RLEPSVAAAKAGKLPEAFFWTDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHIRQR
                     TMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT         4709 a         5164 c         4996 g         4499 t
ORIGIN      
        1 tccttatctt taatttgtat tttgctaaga tttatcttat gctcacaagt cgtatttcca
       61 gagggggata gttaacaatc ttatatttcc ctctgcgcgc ctttcctacc cgcagtatat
      121 agtgcaagcg gtatgaggaa agggagattt cacaaggatg ttatactcaa agtagacaat
      181 cctgatctca agcgtacgta ttgtccatgc gatgaataaa ggaaaagtta tgaaacacaa
      241 gctttctgca atccttatgg ccttcatgtt gacgacgcca gctgcgtttg ctgcccctga
      301 agcaacaaac gggaccgagg caacgacggg tactactggc accacaacga ccactaccgg
      361 cgcaaccacg actgctacta ccactggtgg cgtggctgct ggcgctgtcg gcacggcgac
      421 agttgtcggt gtggcaacgg ctgttggtgt ggcgacgttg gcagttgttg cagcaaatga
      481 cagtggcgat ggcggttctc ataacacctc aaccaccacg tccactacac gctaatactg
      541 ctttaacgcg tcgaaattaa agttaaatga tacccggttg cgatattcgt accgggcttt
      601 agagatttgc atttttttaa ttaacctttc aaggattaaa ggtgcgccct cttattttat
      661 cgattttcgc actatttctt gcgggatgta cgcacagcca gcaaagtatg gtcgatacat
      721 ttcgcgccag ccttttcgat aatcaggata tcaccgtagc ggatcagcag atccaggcgt
      781 tgccttattc cacgatgtat ttacgcctta atgaagggca acgaatcttt gtggtactgg
      841 gatatataga acaagaacaa agcaaatggt tatcccagga taacgccatg ctggttaccc
      901 acaatggacg tcttttaaaa accgtcaaac ttaataataa tctgctggaa gtgacaaatt
      961 ccgggcagga ccctctgcgt aacgcgctgg caataaaaga tggcagccgc tggacgcgcg
     1021 atattctctg gagtgaagac aaccattttc gctctgcgac cctgagttct actttttcct
     1081 ttgctggatt agagacgctg aatattgcgg gtcgcaatgt gctgtgtaat gtctggcagg
     1141 aagaggtgac ttccacgcgg ccagaaaaac agtggcaaaa cacattctgg gtcgattcgg
     1201 ctactggcca ggttcgtcaa agtcggcaaa tgttaggcgc aggggttatt cccgtagaaa
     1261 tgacgtttct taaacccgca ccatgaataa attacagtcg tatttcattg ccagcgtact
     1321 ttacgtaatg acaccccatg cctttgcgca aggaacggtg actatttatc tgcctggcga
     1381 acaacagaca ctttccgttg gccccgtgga aaatgttgtc cagttggtga cacaaccgca
     1441 actgcgcgat cgcctctggt ggcctggtgc cttgctgacc gactctgcgg cgaaagctaa
     1501 agcgcttaaa gactaccaac atgtcatggc acagcttgct tcctgggaag cagaagctga
     1561 cgacgatgtg gcggcaacga ttaaatccgt gcgccagcag ttactcaatc tcaatatcac
     1621 tggtcgctta ccggtcaaac tggaccctga ttttgttcga gtggatgaga acagcaatcc
     1681 cccgttggtc ggggactata cgctctatac cgtacagcgt ccggtgacta ttactctgct
     1741 gggtgcggtt tctggggctg ggcaattgcc ctggcaggca ggccgtagcg ttaccgatta
     1801 tctgcaagat cacccccgcc ttgcaggcgc ggacaagaat aacgttatgg tgattacgcc
     1861 tgagggtgag actgtcgtcg cccttgttgc cctgtggaat aaaagacatg tggaaccacc
     1921 gcctggcagc cagctttggt tgggcttttc tgcgcatgta ctgcccgaaa aatatgctga
     1981 tttaaatgac cagattgtgt ctgttttaac gcaacgtgtt cctgactgat gaagaagaat
     2041 tcttatcttt taagctgcct ggccattgcc gtctccagtg cctgtcatgc tgaagtatta
     2101 acctacccgg atccgctggg ttcgtcgcaa tcagactttg gcggcacagg attgttgcag
     2161 atgccaaatg cgcgcatcgc accggaaggt gaattcagcg tcaactaccg ggataacgat
     2221 caataccggt tctactccac ctccgtggcg ctgttcccat ggctggaagg caccattcgt
     2281 tatacggatg tgcgcacacg caaatatagc cagtgggaag atttcagcgg cgatcagtca
     2341 tacaaagaca aatcattcga ttttaaactt cgcctgtggg aagaaggtta ctggctaccg
     2401 caagtggcgt ttggtaaacg tgatattgct ggtacgggtc tgtttgacgg tgagtatctg
     2461 gtggccagca agcaagcggg gccatttgat ttcaccctcg ggatggcatg gggctacgcc
     2521 ggtaatgcgg gcaatattac caacccgttt tgccgggtga gcgataaata ttgtcatcgc
     2581 gcagagtctc acgatgcggg cgatatcagc tttagcgata tctttcgtgg cccggcttcc
     2641 atctttggcg gcattgagta tcaaacgccg tggaatcccc tgcgtctgaa actcgaatac
     2701 gatggcaaca attaccagaa tgatttcgct ggcaaactgc ctcaggcaag ccatttcaac
     2761 gtcggcgcag tttatcgcgc tgccagctgg gcagatctca acctgagtta tgaacgcggt
     2821 aacacgttga tgtttggctt cacgttacgg accaatttca acgatctgcg ccctgccctg
     2881 cgcgatacgc caaaaccggc atatcaacct gcgcctgaat ctgaaggatt gcagtacacc
     2941 acagtagcga accaacttac cgcgctgaag tacaacgcag gttttgaagc accggaaatt
     3001 cagctgcgcg ataagacgct gtatatgtct ggtcaacaat acaaataccg tgattctcgc
     3061 gaagcggtcg atcgtgccaa ccggattctg gtgaataacc tgccgcaagg cgttgagaag
     3121 attagcgtga cgcaaaagcg cgagcatatg gcgatggtga ctaccgaaac cgacgtagcc
     3181 agcctgcgca aacagctggc tggtacagcg cctggtcaat cagagcaact tcaacaacaa
     3241 cgtgttgaag cagaagatct ttctgccttt ggtcggggct accgtattcg tgaagatcgc
     3301 tttagctact ctttcaaccc aacactttca cagtcgctgg gcggcccgga agatttctat
     3361 atgttccagc tggggctgat gtccagtgcc cgctactggt ttaccgacca cctgctgctt
     3421 gatggcggta ttttcaccaa tatttacaac aactacgaca agtttaagtc ttcgctgttg
     3481 cccgcggact ctaccctgcc ccgcgtgcgc acgcatatcc gtgactacgt tcgcaatgac
     3541 gtttatctca acaacttgca ggcgaactat tttgctgact taggcaatgg tttctatggc
     3601 caggtgtatg gcggttatct ggaaacgatg tatgccggtg tcggttccga gctgctttat
     3661 cgcccgctag atgccagctg ggcgctgggt gtggacgtta actacgtgaa gcagcgtgac
     3721 tgggacaaca tgatgcgttt caccgattat tccacgccaa caggtttcgt gacggcttac
     3781 tggaacccgc cgacgctcaa tggcgtactg atgaaactta gcgttgggca atatctggca
     3841 aaagataaag gcgcaacgat cgacgtcgcc aaacgctttg acagcggcgt ggcggtaggg
     3901 gtatgggcgg caatcagtaa cgtatctaaa gatgactacg gcgaaggcgg ctttagtaaa
     3961 ggtttttata tctcgattcc attcgacttg atgaccattg gacctaaccg caaccgcgcg
     4021 gtggtttcgt ggacaccatt gacgcgtgat ggtggacaaa tgctgtcacg caaataccag
     4081 ctctatccaa tgacggcaga gcgagaagta ccggttggac aataacctga cgtaattcat
     4141 aacattacat tgactgataa atataaacca atgaaaaaga acatttttaa gttttcggta
     4201 ctgacattgg ctgttctgtc tttgacagca tgtacccttg ttcctggtca gaatttatcg
     4261 accagtaaca aggatgtcat tgaactacct gacaatcagt atgacctgga caaaatggtc
     4321 aatatctatc ccgtcacgcc aggattgatt gatcagttgc gcgccaaacc gattatgtcg
     4381 caggctaacc cggagctgga gcagcaaatc gcaaactatg aatatcgtat cggcattggg
     4441 gatgtgttga tggttacggt ctgggatcac ccggaactaa ccacccccgc agggcaatac
     4501 cgtagcgcca gtgataccgg caactgggtt aacgccgatg gtgcgatttt ctacccctat
     4561 attggtcgcc tgaaagtggc cgggaaaaca cttacccagg tacgcaacga gattacagcg
     4621 cgtctggatt cagtcattga aagcccgcaa gtcgatgtca gcgtcgcggc gttccgatcg
     4681 caaaaagcgt acgtcaccgg cgaagtcagc aaatcgggtc agcagccgat taccaatatt
     4741 cctctgacca tcatggatgc gattaacgcc gcgggcggtc tgaccgcaga tgcagactgg
     4801 cgcaacgtgg tgctcaccca gaatggcgta aagaccaaag tgaacctcta tgcgctgatg
     4861 cagcgtggcg acttgcgcca gaacaaactg ctgcacccgg gcgatatttt atttattccg
     4921 cgcaacgatg atctgaaagt cttcgtgatg ggtgaagtgg gtaaacagag cacgctgaaa
     4981 atggaccgca gcggtatgac cctggcggaa gcgctgggta acgctgaggg tatgaatcag
     5041 gatgtcgctg atgcgacggg gatttttgtt attcgtgcaa cgcaaaacaa gcagaatggc
     5101 aaaatcgcca atatttatca gctgaatgcg aaggacgcat cggcaatgat actcggaacg
     5161 gaattccagc ttgaacctta cgacattgtt tatgtcacta ccgcgccgct ggctcgttgg
     5221 aaccgtgtga tttctctgct ggtaccgacg atttccgggg tgcatgacct gacagagact
     5281 tcacggtgga ttcaaacatg gcccaactaa aatttaactc aatcctggtg gtttgtaccg
     5341 gcaacatttg ccgttcgccc attggcgagc gcttgctgcg taaacgtctg ccaggcgtaa
     5401 aagtcaaatc ggcaggtgtt catggtctgg taaaacaccc tgcagatgcg acagcagcag
     5461 atgttgccgc taatcatggc gtatctcttg aaggacatgc cggacgtaag ctcactgctg
     5521 agatggcacg aaattacgat ctgattctgg caatggagtc agagcatatt gctcaggtta
     5581 ccgcaatcgc tcccgaggtc cgcgggaaga caatgctgtt tgggcaatgg ctggaacaga
     5641 aagagatccc ggatccctat cgtaaaagtc aggacgcatt tgaacatgtc tacggtatgt
     5701 tggagcgcgc cagtcaggaa tgggcgaagc gcctcagccg gtaatttgag tttataaaat
     5761 acgatgacaa ctaaaaatat gaatacgcca ccaggcagca ctcaggaaaa tgagatcgat
     5821 ctgcttcgtc tggtcggcga gttatgggat caccgtaagt ttattatcag cgtgaccgcg
     5881 ttattcacgc tgatcgctgt cgcttactcg ctgttaagta caccaattta tcaggcagat
     5941 actctggtcc aggttgagca aaaacagggc aacgccattc tcagcggcct gagcgatatg
     6001 atccctaact catcgcccga gtctgcaccg gagatccaac tgctgcaatc gcgcatgatt
     6061 ctcggtaaaa ccattgctga actgaatctg cgcgacatag ttgagcagaa gtattttccg
     6121 attgtgggtc gcggctgggc gagattaacc aaagaaaaac caggtgagct ggcgatcagc
     6181 tggatgcata ttccacaact gaatggtcag gatcagcaac tgacactcac ggttggggaa
     6241 aacggccact atacactgga aggtgaagag ttcaccgtca atggtatggt cggccagcgt
     6301 ctggaaaaag atggcgttgc gctgactatc gcggacatta aggccaaacc aggaacacag
     6361 tttgtcctga gccagcgtac cgaactggaa gcgattaacg cattgcagga aacctttacc
     6421 gttagcgaac gcagtaaaga aagcgggatg ctggaactta ccatgactgg tgatgatccc
     6481 cagttgatta ctcgtattct gaacagcatc gctaacaact atttgcaaca gaatatcgct
     6541 cgccaggcgg cgcaggattc acaaagcctt gaattcttac agcgccagtt acctgaagtg
     6601 cgcagcgagc tggaccaggc ggaagaaaaa ctcaacgttt atcgccagca gcgcgattcg
     6661 gttgacctta acctggaagc caaagccgtt cttgagcaga ttgtgaacgt tgataatcaa
     6721 ctcaatgagc tgaccttccg cgaggcagag atctcccagc tgtataagaa agatcaccca
     6781 acttatcgtg cgctgctgga aaaacgccag acgctggagc aagaacgcaa acgcctgaat
     6841 aagcgggtat cggcaatgcc ttccacccaa caggaagtgt tgcgtttaag tcgtgacgta
     6901 gaagcgggcc gtgcggtata tctgcaatta cttaaccgcc agcaggagtt gagtatttcg
     6961 aaatccagtg ccattggtaa cgtgcggatt atcgacccgg cagtcactca gccgcagcca
     7021 gtgaaaccga aaaaagcgtt gaatgtggtg cttggtttta ttcttggcct gtttatttct
     7081 gtgggtgccg tgctggcgcg tgcgatgttg cgtcgtggtg tagaagcccc ggaacaactg
     7141 gaagagcacg gcatcagcgt ttatgccact atcccaatgt ccgagtggct ggataaacgc
     7201 acccgtctgc gtaagaaaaa tttattttct aatcagcagc gccatcgtac taaaaatatc
     7261 cccttcctgg cggtggataa cccggcggat tctgctgtgg aagccgtacg tgcgctacga
     7321 accagtctgc atttcgctat gatggagacg gagaataaca ttctgatgat caccggtgcg
     7381 acgccagaca gtggtaaaac gtttgtcagt tcaactctgg cagcggtgat cgcccagtcc
     7441 gatcaaaaag tgttatttat tgatgccgac ttacgccgtg gttattcgca taacctgttt
     7501 accgtgagta atgaacatgg cttgtcggaa tatctggcag gtaaagatga gctcaacaaa
     7561 gtgatccagc attttggcaa aggaggcttt gatgtgatta ctcgcggtca ggtgccacct
     7621 aacccgtctg aactgctgat gcgcgatcgg atgcgtcaat tactggaatg ggcgaacgac
     7681 cattacgatc tggtgattgt cgatacgccg ccgatgctgg cggtgagtga tgccgcggtc
     7741 gtggggcgtt ctgttggcac cagcctgctg gttgcgcgtt ttggcttgaa caccgccaaa
     7801 gaggtgagtt tgtcaatgca gcgtctggaa caggcaggcg tcaatattaa aggcgctatc
     7861 ctcaatggtg tgattaaacg cgccagcacc gcttacagtt acggctataa ctattacggt
     7921 tatagttact ccgagaaaga gtaaaactga aagtggccgg atgcgtcgtg aacgcctttt
     7981 ccggtctacg cattagacag ttcttcgttt gtcatcagcc tcagagcatt caggtaactg
     8041 aatgctcttt tttatgcatt acaaactgca cgccggtatg cgtgcttcat tcacgatttg
     8101 cgtaaaacct gccaacgaac acatgccctg cgcatttcgc tcttcacatc ctgccagggt
     8161 cagtttcacc tctccgggcg gcgtatttaa tgacagcggc gttttatcac gcatctgctg
     8221 taaagtctgg aagaccagcg aaacctgaat ccactggctg ttatcgctta gccgacgcca
     8281 gcgttcaaac accagttcac cacctggcgg cgtgttatcc ggctgaccgg gaagcgtcca
     8341 gttgagctcc agtgcgccgc cgagatttgc cagattagta tcgtgtccgg cgataaacag
     8401 cactgaagtg ggtaatgtca caccatacgc ctgtttttgc ggtggatggg gcgtcaacgc
     8461 tgtcttgatc aaatctaata acggggtggc gcggctgcgg gcaacctctg gcgtgcgttg
     8521 tagcaaataa aattgcgcgt tatgcaaact tagcaaggtg ttccactggt gtgaatcggt
     8581 gatccttccc caccccggct ccggcattcc ctgtgcttgt tgcaggagaa atatctccgt
     8641 cagcattgat gcgaggctta ccgcaccggt taatgagaca ttgtcggcgc tcaccttgag
     8701 ttccgatggt aatgcctgcg ttaatgaaca gctttcgtcc tgtttctcac gtttaaggca
     8761 caagtttgat tgcggaaaat taagcacccg ttccagttcg cgaaacgccg tttgccgatg
     8821 cccggtaaag tcagcaattg accctcctgc cctgctgagg atcgcgtcag tcacgttcgc
     8881 gttatccagt tggcaaacgc cagtttttag aggattaaat aacggatcgg gactggacgt
     8941 atctgcctgg gtatgtacgg ttattgcaca gtcaggtgcc agcccggcgg cgaaggcttc
     9001 gcctgtttta cgggtacgct cgtcgacatc agcaataatc gcgacctgac cagactgcgg
     9061 gcagcccttt ttcgccagca atccgtcggc taccagacgc tggcgttggt aatgtccgag
     9121 ataggcgatt agctcaccac cgcgcggtgt cagccaaccc agttttaccg gccaggttgg
     9181 ccatgcgtct ggggtgacat cctgcatcag ttgcgtggcc ttggttggag cacgcacacc
     9241 atgacgactg acaatcacca cactttccag cttcagctcc ggctcactct gagcgaatgc
     9301 agattgcggg gttaacggaa tcagaagaga taaaaatggg attaagatcg ctttcatcga
     9361 tatgtttccg cttttcatat ctgacattat tgattgcctg atgcgatgct aatgcgccgc
     9421 atcaggccta cgttcgttaa cttttcagac gcgggtccag ccataccaac acaagggtgg
     9481 agagcgaaca catcaacaaa atgccgacga accaaagtaa ataccacatt gttcctgctc
     9541 cttagtacaa ctcgttttcg ttacggcgga gagtttctgt tgtcatgcgt ccccacattt
     9601 tgtagtagct ccagagagtg tagagcaaca caatgggcaa aaatatcagc acgattacca
     9661 acataatgct cagcgtcagc tgactggaag tgctgtccca caacgtcagg ctggagattg
     9721 gactcacgct tgacggcatg acaaagggga acagcgtgat gcctgccgtg aaaatcacgc
     9781 caaattgcat caatgacgcc attaaaaatc cccaacccgg gcgaccacga taaatcgcca
     9841 tcaccgtcag caatgggcag aagaacccca gcagcgggaa gatccacaaa acgggcgatt
     9901 cgacgaaatt attcatccag gcaccaggta gcactgccac cagtttcatt aacggattgg
     9961 aaggaccgtt agcatcctgg gcgagcagta caaagccatc aataccgacc cacagccagt
    10021 aacccgccag caaaaagcac agcatcacca acagtgcagc gcgtttggtg gccagctgtg
    10081 aacgcagatg aatcacacca acagttttca gttgtaacca gacgccacct tgcaaaatca
    10141 ccatcccaag gctgagcaat ccgcacaata aagggaatgg cgtcagcagt tgccagaagc
    10201 tgccgagata ctccacgcgt aattgcggtg tgaaggcaaa cggcacgccg agcaacaagt
    10261 tgccgaaggc gataccgaag actaccggcg gcaccagact gccgatgacc agaccggcgt
    10321 cccacatttt acgccagcgt gcatcggcga tttttccgcg ataatcaaag gccagcgggc
    10381 ggaagaacag tgagcacagc accaggatca tcgccacata aaagccggaa aacgccgctg
    10441 catacactct gggccaggcg gcaaataatg ccccaccagc gaggatcaac cagacctggt
    10501 tgccttccca gtgtgcacca acgctgttta tcactatccg gcgttcatca tcattacgcg
    10561 ccaccagcgg cagcagacag ccgatcccca tgtcaaatcc gtcggagatc ataaagacca
    10621 ccaggatcac gccaatcagc agccaccaga tgaagcgcaa tgtttcataa tcaaacatga
    10681 ttttctcctt tacccctgtt gctgcgtcgg ttgttcactc tgcatcgcgc tcggccccag
    10741 acgggcatat ttctgcatca ggtagacttc ggcgattaag aacagggtgt aaagccctac
    10801 gatcatgatc agtgagaaag ccagttgtcc tgtggttaaa gcggagtgcg cggagtatgt
    10861 cggtaagatg tcctgtatcg cccacggctg acgaccaaac tcggtcataa accacccggc
    10921 ttcaatcgca atccacggca acggcaaact ccagagcgcc attttcagca cccagcgatg
    10981 ctggtcgatt ttgccacgca gcgtctggac aagcgcaatc agcatcacca gtagcagcag
    11041 ggaaccacag cccaccatga tgcggaaact ccagaatacc ggcgcaacct gaggtatcgc
    11101 gccacgcatc gccgcctggt actgtgcggc tgtgacatga ttcatatccg gcgcatagcg
    11161 ggagagcaac atgccgtagc ccaggtcacc ttccagttcc cggaatgcct gcaacacatg
    11221 cggctcacga ttgccttgcg atatttcctg cattaacagc caggccatac gtccgcgttg
    11281 caagcgtggg taggtttcag ccatcaaatt cttcagaccc ggcacgggtt tatctaatga
    11341 gtgagtggcg aggatcccta gcagcgcggg aattttgatg gcaaaggcgt tacgctcttg
    11401 atcctgttcc ggccaggcaa ccacatggaa cggtgcaggt gcaggttccg tttgccactc
    11461 cccttccatc gccgccagtt ttaccggttg tacttgcgcg acttcatacg cagaactgtc
    11521 tccgagttgc agggtaccga taatcgccag agtaccgaag acggaaccga tggcaaacga
    11581 acgtaatgcg acatcgcgct cccgtccgcg cagtaaatac caggcgctga tcgccataat
    11641 aaacatggcc ccggtcacgt agcccgccat tacggtgtgc acaaatttca cctggctgac
    11701 cgggttaaag accagctcgc tgaagctggt catctccata cgcagggtgt cgatatcaaa
    11761 atgcgcaccg gtcgggtatt gcatccaacc gttggcattc aatatccaca atgcagagag
    11821 atttgaaccg aacgccacca gccacgtcac cagcaggtgc tggtatttat tcagccgctg
    11881 ccagccgaag aagaacagcc cgacaaaggt ggattcgagg aagaaggcca ttaatgcttc
    11941 catcgccagt ggtgcgccaa aaatatcgcc cacatagttg gaatagaatg accagttagt
    12001 accaaactga aactccatgg tcaggccggt agccacgcca agagcaaaat tgataccgaa
    12061 gagcttaccc cagaagcgcg tcatatcgcg gtagattgtt ttgccggtga ccacgtaaat
    12121 ggtttccatg atagccagca aaaaaatcag ccccagggta aggggtacaa ataaaaagtg
    12181 atacagcgcg gtcagagcaa actgccagcg cgataaatca atgacatccc acatgcgcac
    12241 tcctgtaggg gaagagagaa aaataacccg ataatggtag atctccctct ttatcctgaa
    12301 actctacgac cagaggtatt cgccgattgc gatattcata agactgaaag cgatacttac
    12361 cgtcttttta cgtcggtgca gcttccgcca gccactgaca tacctcgcta agccgctgcg
    12421 cagagtcgac caaatcttct ggcgctgcca gcaccacttc cggtattggg ctgatttcat
    12481 aactttccag taacgggcct tttaaggtgt ccgtacagcg taaatgccag acatggcgta
    12541 accccgtggc gttgatatag ctctcgccat agccaatggt acgaatctga atatttcccg
    12601 gcccacagag acgtgagaga aaaagccgat cagcctcgct gatgggtaat tgcgtcagat
    12661 taatgctgtg cggctgcgcg tcggggttac gtacatgtgc cagtaactca tgcgccaacg
    12721 gtaggctatt catcaggcca tcaatgggcg gcggtaacag cgaatctgtc ggcaagagat
    12781 tttgcgttgc cgcctgccac aacgccagcg gcgcgcagcc agcctccagt ttgtcctcca
    12841 gcaacttttc gccgcgacgt ctgcgcaccc gccataatcc gcagaagatc gcctcctgta
    12901 tttcactttc actgtcgtca gcctgctgaa tgcgcactga cacttcccct tccccgagaa
    12961 gcgtattgat aaagtgacga tcgtcggcgt tgagatttaa cagatcgact gccagcacct
    13021 cgcccaacgg cggttggcgt tcgcttagtt gatgttgtaa ctcgtttaac agcgcaatca
    13081 cctgcgggct gtgagcacat tgctccaggg ccgccatact cggttcatca ttcacctgac
    13141 aggtgatcgg cagtggattc atgctgaaac tgtcatcgtt cggttgcgtt cctggcccca
    13201 gcagatggaa aaaagtttcg ctcatgaggc acgctcctgc tgcgattcga caagcccgcg
    13261 catcaggttt atcagttccg cccacgggtg aataccattc agcacgccgc gatagtttcc
    13321 gccggtaaac actaaagtgg caggaaagcg aaagacgcca aagcgatcgc cgatggcttc
    13381 gctctgctca aggtcagcaa tcgccacctg ccatgtatag tcgggaaact cgcgcagtaa
    13441 ttcgccaatc attaccggat tatcgctgac ctctggcgtg cgtttcgggt cactgcttaa
    13501 taacaccacg ccgtctggcg cttgcgtaag ccagtcgtca agacgggatt cactgactgg
    13561 cgtccagccg cgcgccagca ttcgttgcca caacgcatca aatggcgtgt cgttgctcat
    13621 ccctgctcct cctgtgtcat ccggtactgg cgcaagcgaa cgccttcgta attttccatc
    13681 gacagacagt cataattgag acagcgcgat tcattagccg gttgcggcac aattccccac
    13741 gcagccagct gcgccagcgc cgcctgttcc gcagcgggca gttgctcccg tgccagttcg
    13801 ctcaggctac cgccgtagtc gtcgagcatt gcgggttgca gaccgacgag ggcaatatgt
    13861 gctggcagat gtccgcggat atccgccagc gccaacactt cggagaaact gttctgatgc
    13921 aggctcattt tcttcgcgct gagataagcc ggaatgcgtt ctccggcata ggttcgcagc
    13981 gttccaggtt ccagcccgta gtcaatggca tcgagaatca acagatggct ggcgctttcg
    14041 acatacccca gcaagttcag tccctgagta ccgccatcga caatctccac atactcgggc
    14101 cagtggtaat gggcatacag ccgttccgcc acccgcacgc cgaagccttc atcggcccac
    14161 agcaggttgc ccagccccat gaccaccacg cgttgctcgc tcatgaacgc tccttgttac
    14221 ttattttgcc aaatttgtgg ctacggtagc cgttgaccat ggtggagatc accgtgtcgt
    14281 cggacatgat gtcttcacgc agtgccatgt agacatgacc gatcacaaac gcgccaatca
    14341 gccacatccc cagccgatgc cagctgtgaa tgtccattga gttgccaccc gtccagtaga
    14401 aaaattccac cacataacgg aacggcgcaa aaatagcgta ctggctgtgt tcgctgtaca
    14461 gcgcaaaacc agtgatgatc ataaagaccg acatcaggaa atagccgaac atcgccgcct
    14521 gggcgatggg attatggcct atatcggcac tcggacgttt tgccagaaac agataccagc
    14581 ggatttcata ccacacgccc tgccaccagc ttttacgcca taccggcacg ataaacagct
    14641 cgcgggagta tcgattgcca acaaaagccc agtagatccg catcagcaaa accacggtaa
    14701 aaaccatccc ggcgctgaag tgaattaacc tgatgtagcc catatagaac agatacgtcg
    14761 cctcgccgct gacggaaggt agcggcttgc cgataaagta tccggtgacc atcaacaccg
    14821 ccatgcataa caccgtcaac cagtgccaga tgcggactgg cgcttcaaag acatagtggc
    14881 tgacaacgtt gtcgcttttc tgttgcatga tgattctcct tcgctgttaa cgcacctgca
    14941 cggagatcag ctcgctaccg tcgtcgccca gcacgtgtgt tgaacaggcg aggcacgggt
    15001 caaagctgtg cagagtacgc aggatctcca gcggttgctc ggggatcgcc attttggtgt
    15061 tcatcagcgc cgcttcataa gcgccaatct gccctttggg atcgcgcggg ctggcgttcc
    15121 aggtggtcgg caccacgcac tggtagagat caatcttgcc atcgcgaatg gcggcccagt
    15181 ggcctaacgc cccgcgcggc gcttcggtaa aaccgacacc acggcactct gtcggccagg
    15241 ttgcaggttc ccatttttcc gtggaagcag tggcgagatt gccgtttttc aggttggtca
    15301 tcagcttgtc gaagaaatac tgcaacttac ctgcggccca ctgcgcttcg tgcgcgcggc
    15361 acaaaatgcg gcctaacgtt gactggatac cggaaagcgg caggttcaac gccgacatca
    15421 tgcgatcgac cgactcaacg gtcgcagcat cgcctttgtg ataagcgatt aacgtgcgtg
    15481 ccagcggccc cacttccatc gcgttaccgc gccagcgtgg cgctttgatc cacgagtagc
    15541 gttcctgttc attcagctgc tgaatgttgg tatcgctgcc tttgacatcg ccggggttgt
    15601 accacgggtc ggtaatgcca tcgaatggat gacgcccgac ctgatcgttg ggataacgat
    15661 accaggcgtg atcgacaaac tcctgcacct gctgcggatc aaccaaatcc actggcagca
    15721 cattgttgaa gtcgccgtta atcaccgcgc cgccaggcat cagcagactt ttctcaccaa
    15781 agtcgttggc aatatccggg aatgcgccgt agctgaggac acatttatca gaaagacccg
    15841 tgccgatttc gctccacggt ttgttgaact gaccgatggc taaggcgtcg gggatcatca
    15901 cgttgttaat gaagtctgcc gtgcgggtga taatcgactg caccaggttc aggcgttcca
    15961 tattgaccgc cccgaccgcg ccgctttcgt caatgttaat ggcgcaaggc atcccgccga
    16021 caatccagtt tggatgcggg tttttaccgc caaagaccgc gtggattttc acaatttcac
    16081 gctggaaatc gagagcttcg aggtagtggg caaagcccat caggttagct tctggcggca
    16141 gtttgtactg cgggtgcccc cagtagccat tgcggaagat ccccaactgc ccgccttcaa
    16201 cgaatttctt caggcgattt tgtacgtcga agaaatagcc aggggatgat ttcggccatg
    16261 aggagagact ttgcgccagt tcggaggttt tccgcgggtc ggctttcagc gcatctaaca
    16321 catcgatcca gtccatcccg gcaagctgat agaagtgcac caggtgatcg tggcaccaga
    16381 gcgttgccag cataatgtta cggatgatat tggcgttgtc cggcacttta ataccgatgg
    16441 catcttcgat ggcgtaaact gaagccaggg catgtacgcc agtacagacg ccgcagatac
    16501 gttcaacgaa cgcccacgca tcgcgcgggt cgcgcccttg caggatgatc tccagcccgc
    16561 gaaacatggt gccgcaggaa acggaattgg tgatcacatt ctgatcgtta atattcactt
    16621 cgcagcgcat gtggccttcg atgcgcgtaa tcgggtcaac caccaggcgg cgtccggcat
    16681 tattgatggt gtatccctga gtttcgtact gagtgctcat gcctgtttat cctcattgcc
    16741 tggctgatgt tcggtttctg taggttgctg gttatggcgt ctgcgctggt caacggcgct
    16801 ggcgactgcg tgcacaccaa cagccgctgc caccacgcca agtgcggtta aaccgacggt
    16861 atcggcggtg gaatgggtac ccatttgcgg aatatcgacc acgcggctgt agaacgaacc
    16921 tcgatcccag aaaccatttt ccgcacagcc caggcagccg tgaccagact ggattgggaa
    16981 agaaacgcca tcattccagc gggtggagga acaggcgtta taggtggttg gccctttgca
    17041 acccattttg tacaggcagt aacctttgcg ggcagcgtca tcatcccaac tctggacgaa
    17101 ctctccggcg tcgaagtggg cgcggcgata gcatttatcg tggattcgct gaccatagaa
    17161 catcaatgga cggcccattc tgtcgacatc tggcaagcga tcaaaggtca ccatgtaagt
    17221 aatgatggcg ctcatcacat ccgggatcgg cgggcagcca ggtactttga taatgggttt
    17281 gtcggtgatg actttgtcga taggcgttgc ctgcgtcgga ttgggccgcg cggcctgcac
    17341 gcagccccag gacgcgcagg ttccccaggc gataatcgcg ctggctccgg cagcggcacg
    17401 tttgagtttc tcaataaacg gtcgaccgct gctgatacag aacatcccct gctcgcccag
    17461 cggcggatta ccttctactg cgaggatata tttgccattg tattgcgcga tgatgtcttc
    17521 aaagacttct tccgcctggg ttccggcggc agccatcaaa gtatcgtcgt aatcgaggga
    17581 aatcagggaa aggatgacgt ccttcgccag tgggtgagcg gagcggataa aagattcggt
    17641 acagcaggtg cattccagac cgtggatcca taccaccgga atgcggggtt tgttctccag
    17701 cgcccaggca atctttggtg ccattcccgc gcctaatccc agcgacgtgg cagccagact
    17761 acaatatttg agaaagctgc gccgggtaac gccctgacgc cgcatggcct ggtaaaatgt
    17821 ttcctcgtta ttcatatcgc acgtctcttc tccttgcgac accggcagga caccgggcgc
    17881 acatggtcgg caagcgacgt ccctgtagca aggcaaacca tcgcttcacg tgagatgctg
    17941 aaaacgaaag ctcatccttc tgcactggca cacgtcgcca gaaagtattg ttaataaagc
    18001 gtagtgaaac ttttgcacaa aacaatacaa actgtgtgga tttatctttt agcgataaaa
    18061 atggacctat ttttcttttg gccgggcggt ggggatgttt agccggttgc taaacgagta
    18121 aaagagaagg aattcatggt gatgaaatga tcaatctgct gttatttcac gctatcaatg
    18181 cacttttggc tgtttttcag gcaaacaaac aaattagggg tttacacgcc gcatcgggat
    18241 gtttatagtg cgcgtcattc cggaagtgtg gccgagcggt tgaaggcacc ggtcttgaaa
    18301 accggcgacc cgaaagggtt ccagagttcg aatctctgcg cttccgccaa acaatcaagg
    18361 ggttaccaaa tggtagcccc tttccttttt ttgctcttag tatacacttg gtatactaat
    18421 tggtatattt agctcaatac acactaggta cattgttctc tttacgattt atcgctatac
    18481 cgatgaatta tgaataaccc caatccatgc atgacgtgtg gtgcctgttg tgcatttttc
    18541 cgcgtctctt tttactgggc agaagctgac gatgctggcg gaaaggttcc ggtcagtctc
    18601 actgagcaaa tatccccctt tcatcgctgt atgcgcggca ccagtcagaa aaatccccga
    18661 tgtgtcgctc tggcgggaac tccgggtaaa aacgcatatt gctctgttta caaaaaccgt
    18721 ccgtccacgt gccgggaatt cgcaatgtcc ggagaaaatg gcatcgcaaa tgaagcatgc
    18781 aaccgcgccc gggcaaaata cggacttccg gaaatctgaa aaatcatcca tatcaacagg
    18841 atgcgcgaaa aatgagagtt ccgtcacttc cggaattaac ttttccgtgg cactatttac
    18901 ccggcatagt gtatatcttc acaataatga cataatcaac gcagttcatg ggggaggtga
    18961 tggtccccca tctttttgtc tgttattcct gtgcccagcc aaccctgtat gccagtatct
    19021 catccgcact ggtcagcttt tccagctcct tcttcatggt gcgctggcga atgtggattt
    19081 ccatcccttt agtgaacatc gcctgctctg ccgcttcact cagcgcaata agctcttctg
    19141 ctgtcaccgg cacatcattg ttttccgcat ccgtccagaa aaacgcctcc ggcagtttcc
    19201 ccgctttcgc tgcagccacc gacggctcaa gacgcgtctg cgttgacttc ccgtagtccc
    19261 atttacgccc attgtgctca aacgtgtagt tcgccgcttc catcgcatta cgccaggcgt
    19321 taatttcatc gcccttcatc cctcgcgctt tctccgcggt cagcaggt
//
LOCUS       DAZFFI010000085        19003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFFI010000085 DAZFFI010000000
VERSION     DAZFFI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.085"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..588
                     /gene="yfcV"
                     /locus_tag="ACHJ12_003517"
     CDS             25..588
                     /gene="yfcV"
                     /locus_tag="ACHJ12_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991251.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            670..3315
                     /gene="yfcU"
                     /locus_tag="ACHJ12_003518"
     CDS             670..3315
                     /gene="yfcU"
                     /locus_tag="ACHJ12_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO4991252.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDEARKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3335..4087
                     /gene="yfcS"
                     /locus_tag="ACHJ12_003519"
     CDS             3335..4087
                     /gene="yfcS"
                     /locus_tag="ACHJ12_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4991253.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4062..4610
                     /locus_tag="ACHJ12_003520"
     CDS             4062..4610
                     /locus_tag="ACHJ12_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991254.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4607..5077
                     /locus_tag="ACHJ12_003521"
     CDS             4607..5077
                     /locus_tag="ACHJ12_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991255.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5074..5598
                     /locus_tag="ACHJ12_003522"
     CDS             5074..5598
                     /locus_tag="ACHJ12_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991256.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5585..6457
                     /locus_tag="ACHJ12_003523"
     CDS             5585..6457
                     /locus_tag="ACHJ12_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4991257.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6579..7130)
                     /gene="smrB"
                     /locus_tag="ACHJ12_003524"
     CDS             complement(6579..7130)
                     /gene="smrB"
                     /locus_tag="ACHJ12_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4991258.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7296..8228
                     /gene="prmB"
                     /locus_tag="ACHJ12_003525"
     CDS             7296..8228
                     /gene="prmB"
                     /locus_tag="ACHJ12_003525"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4991259.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8263..9348
                     /gene="aroC"
                     /locus_tag="ACHJ12_003526"
     CDS             8263..9348
                     /gene="aroC"
                     /locus_tag="ACHJ12_003526"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4991260.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9352..10176
                     /gene="mepA"
                     /locus_tag="ACHJ12_003527"
     CDS             9352..10176
                     /gene="mepA"
                     /locus_tag="ACHJ12_003527"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4991261.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHRTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10176..10985
                     /gene="yfcA"
                     /locus_tag="ACHJ12_003528"
     CDS             10176..10985
                     /gene="yfcA"
                     /locus_tag="ACHJ12_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4991262.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10985..11533
                     /gene="epmC"
                     /locus_tag="ACHJ12_003529"
     CDS             10985..11533
                     /gene="epmC"
                     /locus_tag="ACHJ12_003529"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4991263.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11567..11845
                     /gene="yfcL"
                     /locus_tag="ACHJ12_003530"
     CDS             11567..11845
                     /gene="yfcL"
                     /locus_tag="ACHJ12_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4991264.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(11966..13972)
                     /gene="mnmC"
                     /locus_tag="ACHJ12_003531"
     CDS             complement(11966..13972)
                     /gene="mnmC"
                     /locus_tag="ACHJ12_003531"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4991265.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14131..15351
                     /gene="fabB"
                     /locus_tag="ACHJ12_003532"
     CDS             14131..15351
                     /gene="fabB"
                     /locus_tag="ACHJ12_003532"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4991266.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15644..16822
                     /gene="yfcJ"
                     /locus_tag="ACHJ12_003533"
     CDS             15644..16822
                     /gene="yfcJ"
                     /locus_tag="ACHJ12_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4991267.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16819..17814)
                     /gene="flk"
                     /locus_tag="ACHJ12_003534"
     CDS             complement(16819..17814)
                     /gene="flk"
                     /locus_tag="ACHJ12_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4991268.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(18082..18975)
                     /locus_tag="ACHJ12_003535"
     CDS             complement(18082..18975)
                     /locus_tag="ACHJ12_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HFO4991269.1"
                     /translation="MKNIIFSIGASFISISVLPVHAATEHNNFNAVLQCRAIENNKDR
                     LSCYDKSIQPTRTKVAEKFESRDQCPDEKDDDRRLSCYDRFFSPTFTPSVNSKSKTEK
                     PVTTEAQQPNLSEISKCRAENDKEARLNCYDKLFPQDKAVQAESKLEKATDVGKWHTS
                     ITTSPIDDSKNVILSLESDDYIRTPFGEAVTPTLFIACREKKTEVFLSWDVYLGLEQT
                     SMLYRLDKQKAVERNWLVSTDTKAVFYKGNDIDFIRKLANSNKMYTKITPYNESPVSA
                     TFNLNGLSNALKPLQAACNWK"
BASE COUNT         4547 a         4703 c         4943 g         4810 t
ORIGIN      
        1 ttatttatag tttaaggaca aataatgagt aagtttgtta aaacagctat tgctgcggca
       61 atggtgatgg gcgttttcac ctctacggca acaatcgctg cgggtaacaa cggtactgca
      121 cgtttttacg gcaccattga agactcagtg tgttctatcg tcccggacga tcacaaactg
      181 gaagttgata tgggtgatat cggtgccgaa aaactgaaaa ataacggcac caccacgccg
      241 aaaagtttcc agattcgtct gcaagattgc gtatttgata ctcaggaaac aatgactact
      301 acctttactg gtaccgtttc ttctgcaaat agcggcaatt attacaccat tttcaatacc
      361 gataccggtg cggcatttaa caatgttagc ctggcgatcg gtgactctct gggtacctct
      421 tacaaaagcg gcatgggtat tgaccagaaa atagtgaaag acacttctac caataaaggc
      481 aaagcgaagc agacactgaa ctttaaagcc tggctggtcg gcgcagctga tgcgccagat
      541 ctgggtaatt tcgaagcaaa taccaccttc cagattactt acctgtaagg cataccccca
      601 tagcgacact gctatggggg atttaaatgg attttgaccg aacaagaata aaaacggata
      661 gaaacgtgta tgcctgacca ttctcttttt cgattacgga ttcttccgtg gtgcattgcg
      721 ctggcaatgt cagggagtta tagcagtgtc tgggctgaag acgacattca gtttgattcc
      781 cgttttctgg aattaaaagg cgacacaaaa attgatctga agcgtttttc cagccaggga
      841 tatgttgagc ccggaaaata caatttacag gttcaactaa ataaacagcc attggcggaa
      901 gagtacgata tttactggta tgctggtgaa gatgaggcga gaaaaagcta tgcttgtctg
      961 acaccggaac tggtagcgca gtttggttta aaagaagacg tggcgaaaaa tcttcaatgg
     1021 agccacgatg ctaaatgcct gaaatccggt caactggaag gcatggaaat taaggctgat
     1081 ttaagccagt ccgcattagt catttcactg ccacaggctt acctcgaata tacttatccc
     1141 gactgggatc cgccttcacg ttgggatgac ggcatctccg ggatcgtcgc ggactacagc
     1201 atcaacgcac aaacccggca tgaagaaaat ggcggtgatg atagtaacga gatcagcggc
     1261 aacgggacgg tcggggttaa cctggggccg tggcgtatgc gtgctgactg gcagactaac
     1321 tatcaacata ctcgcagtaa tgatgatgac gatgaattca gcggcgatga aactcaaaaa
     1381 aaatgggagt ggagtcgcta ctatgcctgg cgggcgttac catcattaaa agccaaactg
     1441 gcgctgggcg aggattacct cagatccgat atttttgatg gttttaacta tgttggtggc
     1501 agtgtcagta ctgacgatca aatgttgcct cccaatctgc gcggctacgc gccagacatt
     1561 tccggcgtgg cacacaccac agcaaaagtg accgtcagcc agatggggcg tgtgatttac
     1621 gaaacgcagg ttccggctgg accgtttcgt attcaggatc ttggtgattc tgtctccggt
     1681 acgttgcata ttcgcattga agaacagaac ggccaggtgc aggaatatga catcagcacc
     1741 gcctcgatgc catacctcac tcgcccaggt caggttcgct ataagatcat gatgggccgt
     1801 ccgcaagagt ggggacacca tgtcgagggt gaattttttt ctggtgctga agcttcctgg
     1861 gggatcgcta acggctggtc gttatatggc ggcgcactgg gagatgaaaa ctatcagtct
     1921 gcggcgcttg gcgtcggtcg cgatttgtct acattcggcg cggtcgcgtt tgatgttact
     1981 cactcgcaca ccaaactgga taaagacacc gcttatggca aaggttcgct ggacggtaac
     2041 tccttccgtg tgagttattc caaagacttt gaccagctca acagccgcgt tacttttgct
     2101 ggatatcgct tctcggaaga gaactttatg accatgagcg agtatctgga tgccagtgac
     2161 agcggaatgg tacgcacggg caacgacaaa gagatgtaca ccgccactta taaccagaac
     2221 ttccgcgatg cgggtgtttc ggtttatctc aactataccc gccataccta ctgggatcgc
     2281 gaggagcaga caaactacaa catcatgctc tcgcactatt tcaatatggg cagtattcgt
     2341 aatgtcagca tctcgatgac tggctaccgt tacgagtatg acaaccaggc cgacaaaggc
     2401 atgtacattt cgctcagtat gccgtggggc gacaacagta ccgttagcta taacggtaac
     2461 tatggcagtg ggacggacag cagtcaggtc ggttatttca gccgtgtcga tgacgcgact
     2521 cactatcagt tgaacgtcgg caccagtgac aaacacacca gcgttgacgg ctattacagc
     2581 catgatggtt cgctggcgca ggttgacctc agtgcgaact accatgaagg gcaatacacc
     2641 tctgcgggct tgtcgttaca gggcggcgca acgcttactg cccacggtgg cgcacttcac
     2701 cgtacccaga atatgggcgg gacacgcttg ttgattgatg ccgatggcgt tgccgatgtt
     2761 ccggtagaag gtaacggtgc tgctgtttat accaatatgt ttggtaaagc cgtcgtttct
     2821 gacgtcaata actattaccg caatcaggcg tatatcgacc tcaacaaact gccggaaaac
     2881 gccgaagcaa cccagtcggt ggtacaagcc acgctaactg aaggggcgat tggctaccgc
     2941 aaatttgccg tcatcagtgg tcaaaaagcg atggcggtgc tgcgtttaca agacggcagc
     3001 catcctccgt ttggcgcaga agtaaaaaat gataacgagc agacagtggg ccttgtcgat
     3061 gatgacggca atgtttatct ggctggggtg aaacctggcg aacacatgag tgtgttctgg
     3121 agtggtgttg cgcattgcga tatcaacctg ccggacccgc tgcctgccga tctgtttaac
     3181 ggcttgttac tgccatgcca gcataaaggc aacgtcgcgc cggttgttcc tgatgacata
     3241 aagcctgtca ttcaggagca gacgcaacag gtgacaccca ccaatccccc tgtttctgtt
     3301 tcagctaacc aataacgtga ttaaggaatg atccatgtct gatttacttt gctctgccaa
     3361 attgggtgct acgacgctag cgttactgct tagcgctgcc agtttgtctg cccaggcttc
     3421 tgtaacgcca gatcgtaccc gtttgatatt taatgaaagc gacaaatcaa tcagcgtgac
     3481 gttgcgcaat aacgatccga aactgcctta tctggcccaa agctggatcg aagatgaaaa
     3541 gggtaacaag atcagttcac cgttaactgt tctgccaccg gtgcaacgca tcgattcaat
     3601 gatgaacggg caggtaaaag tgcagggaat gccggatatc aacaaattgc ctgccgatcg
     3661 tgaaagcctg ttctacttca acgtgcgtga gatcccgcca aaatcaaaca aagccaacac
     3721 gctgcaaata gcactacaaa cgcgtattaa gttgttctgg cgaccgaagg cgctggagaa
     3781 cgtcagcatg aaaaacccct ggcaatataa ggttacttta acccgcaacg gtcaggaatt
     3841 taccgtcaat aacccgacac cgtattacgt cattttcagt aatgccagca cacagaaaaa
     3901 tggcaatccg gcagccggtt tctcgccaat agtgatgtcg ccgaaaacct ctgcaccgct
     3961 gaatgtgaag atgggtagcg tgccggtact gacctatgtc aacgactatg gcgctcggat
     4021 gccgctcttt ttcacttgca acggtaacag ttgccaggtc gatgaagagc agagccgcaa
     4081 aggctgaggc cgggagagcc agtatgaaag gatcttgttc tgtctgggcg gtaactgggt
     4141 tactgatatg ttgtgttggt agcggctttg cagttgcagc gagtaagtca gtaagtatga
     4201 atttacgggt gcttgtcgat gcccctcctc cttgtacggt aaatggtgcg gcagttgagt
     4261 ttggtaatgt attcattaat aagatcaatg gggttgatta taaacgtccc attgactact
     4321 cgcttgtttg taacaatctg gcaatggatg acctgcgtct acagatgcag gcgacaacgg
     4381 tcgtgattaa cggtgaaaca gttatcagca cggggattcc tgggtttggt attcgggttc
     4441 agaaaagtag tgatcacaca atactcgact taacctctgg ctcatggttg ccatttaatt
     4501 tttcctcggg agttcccgtt ctggaagctg tgccggtgaa acagagtggt acaacgttag
     4561 cggcagctga atttaacgcc agtgccacaa ttgtggtgga ttaccaatga aacggatctc
     4621 gcttattttg ctgtggggct tttgctcgat ggcactctca aacgtctctt ttcacggtta
     4681 tttagtgcag ccacctaact gcacgattag caacgctcaa accattgaaa taacttttca
     4741 ggatgtgctt attgatgaca ttaatggcag caattatgag caaaccgtgc cgtattccat
     4801 tacctgcgat acggcggttc gcgacccatt aatggaaatg accttgagct ggagtggcac
     4861 accaagcgat tttgataatg ctgcagtaag cagcaatatc actggtttag gcattcagct
     4921 caaacaggca gggcagagtt ttacgatcaa tacgccactt gtggtgaatg aaaccgattt
     4981 accggtgtta acggctgtgc cagtaaaaaa gagtggtgtc gttctgccag aggcggactt
     5041 tgaggcctgg gccacgttac aggtagatta ccaatgaaga aaaagcgtac gttgtttttt
     5101 attagtagcc tgatgttgct gggcagtgga accacaatag caggagataa tttgcacttt
     5161 accggcaatt taatcagtaa atcgtgtacg ccagtgatta atggtagcca gttggcagaa
     5221 gtacattttc ctgccattgc cgccagcgat ttaatgaacc ttgggcagtc agagcgagtg
     5281 ccattggtgt ttcaattgaa agattgtcat agttctacat tgtttaatgt caaagtaacg
     5341 ttgacaggaa cagaagatag tgctctaccc ggctttctgg cctttgactc ctcgtcatca
     5401 gcatccggcg ctgggattgg tattgaaact gcggcaggca catcggtgcc aatcaacaat
     5461 acaacaggcg tcacgctccc gctgaatcag ggaaataaca gcctaaattt taatacctgg
     5521 ttacaggcaa agagcgggcg agatgtaacg tccggagact ttagtgccac agtgacggca
     5581 acctttgagt atttctgaaa tgaaaaaatg gaccataatt ttcgcgagcc taatgctgct
     5641 cgttctgagt gttgtcggcg catctaagag ttatgctgga gataaactta tgagtgcgag
     5701 ttttgacagt gtgagagttt attatgccat ggacaaggta accggtgctg tagcgagcag
     5761 tgttttcaat gttacggtga taacgccaaa ggaggttgca tatgggaaat atgattcttt
     5821 tgcgtataaa ggcgatacgt tgcgggttat ttcctggtct gggtcgggtt cagctccaac
     5881 tttggtactg actgactttg atacaattaa taactcaaat tgccctggca tagatacgaa
     5941 aatttttcgt tgtgcatata tgacgtttaa aattaccgtt gcatctgatg attatggttg
     6001 cccttggatc gcatcctttt attcctatac agatttaccc ggatttggaa gctatacggc
     6061 ccctacagtg cataacacaa tatgcccaac gataccggtc gcttcatacg atatctcctg
     6121 gagtgaaaat tatgtcagcc ataataaggc gttacgaatt cagtccacgg ggtcaacagt
     6181 gaccaccacg ctttcgacct acttgatgga aggcggcaga ctctgtgatg gcagtaattt
     6241 ttctgataat gatggcagag gagcatactg ccgcgcggta tcagaattgt taacctttac
     6301 ctcttacggt tgtgataaat caacggtaac ggttacgcca actcgtcatc cggtaacgga
     6361 taaagtacta cacgatattg tggtgaatgt gaataccagt agtgggcaac cgattgattc
     6421 gacgtgtcgt tttcagtatg tattgaatga actgtagttc gtagtattgc tctgatgcgt
     6481 cttatcaggc ctacgaagtg aattgcaatt tattgaatta tcacatattt gtaggccgga
     6541 taaggcgttt acgctgctcc ggcatttgct gccagcattc agggcaactc cggcggcaac
     6601 cactcttcca cttcaatcaa caccaacaaa gccgcatcac cgccgtactc tttcggtgcc
     6661 tggtgaaatg ccatcacatg cggatgttgt gccagccaca gcggtgtttg ttgcttcaaa
     6721 atatgcttcc cgtgcccatg catcacgcag gcacaaaaca catgttcgcg gcgacaggcg
     6781 gcaatcaacg cgcccagttc ctgcttggcc tgcagctgcg tcagaccgtg taaatccaaa
     6841 aacaattccg gtgaataatc gccgcggcgc agtttcttcg cctcaaaatg gctgacatcc
     6901 gggcgaacat atttcaccgg accttcggta tttaataacg gctgaaactc atcggagaaa
     6961 taatggctgg catcagcctg ctcctggatc aagcgtttca ccggcacttc gctgattttt
     7021 ttacgctgcg gtcggtggac aatcgtgtcc tgcttaatct tgcgagtccc cgccatcaac
     7081 tggcgaaaca gagcctggtc ctcctcgcta agtgttgttt tctttttcat ttatctgtct
     7141 ctatttctaa ttttcctaca gtttacccga ctcgtagcac aatcgatcgt tttaaacgta
     7201 tttttcctca ctcaccgcgc gtgaatgctg atttatcgtc atcttcatgg caaactagcc
     7261 gccgaaatta ttgcgatcat gccctggagg aatacgtgga taaaattttc gttgatgaag
     7321 cagtaaatga gctgcaaacc attcaggaca tgttgcgctg gtcggtaagc cgcttcagcg
     7381 cggcaaatat ctggtacggt cacggcaccg ataacccgtg ggatgaagcc gtacagctgg
     7441 tgttaccatc gctctacctg ccgctggata tcccggaaga tatgcgcacc gcgcgtctga
     7501 cctccagcga aaaacaccgt attgttgaac gcgtgatccg ccgcgtcaac gagcgcattc
     7561 cggtggctta cctgaccaac aaagcctggt tctgcggtca tgaattttac gtcgatgaac
     7621 gcgtgctggt gccgcgctca ccgattggtg aactgatcaa caataaattt gccggactca
     7681 tcagcaagca accgcagcat attttagata tgtgtaccgg tagcggctgc atcgccattg
     7741 cctgtgctta tgccttcccg gaagcggaag tcgacgcggt agatatctct cccgacgcgc
     7801 tggcggttgc cgaacagaac atcgaagaac acggtctgat ccacaacgtc attccgattc
     7861 gttccgatct gttccgcgac ttgccgaaag tgcagtacga cctgattgtc actaacccgc
     7921 cgtatgtcga tgcggaagat atgtccgacc tgccaaacga ataccgtcac gagccggaac
     7981 taggcctggc atctggcacc gacggcctga aactgacgcg tcgcattctc ggaaacgcgg
     8041 cagattacct tgcggatgat ggcgtgttga tttgtgaagt cggcaacagc atggtacatc
     8101 ttatggaaca atatccggat gttccgttca cctggctgga gtttgataac ggcggcgatg
     8161 gtgtgtttat gctcaccaaa gagcagctgc tggcggcccg tgaacatttc gcgatttata
     8221 aagattaagt aaacacgcaa acacaacaat aacggagccg tgatggctgg aaacacaatt
     8281 ggacaactct ttcgcgtaac caccttcggc gaatcgcacg ggctggcgct cggctgcatc
     8341 gtcgatggtg ttccgccagg cattccgctg acggaagcgg acctgcaaca tgacctcgac
     8401 cgtcgtcgcc ctgggacatc gcgctatacc acccagcgcc gcgagccgga tcaggtcaaa
     8461 attctctccg gtgtttttga aggcgttact actggcacca gcattggctt gttgatcgaa
     8521 aataccgacc agcgttctca ggattatagt gcgattaagg acgttttccg tccaggccat
     8581 gccgattaca cctacgaaca aaaatacgga ctgcgcgatt atcgcggcgg tggacgttct
     8641 tccgcccgcg aaaccgccat gcgcgtagcg gcaggggcga ttgccaaaaa atatctcgct
     8701 gagaaatttg gtattgaaat ccgcggctgc ctgacccaga tgggcgacat tccgctggaa
     8761 atcaaagact ggtcgcaggt tgaacaaaat ccgttcttct gcccggaccc ggataaaatc
     8821 gacgcgttag atgaactgat gcgcgcgctg aaaaaagagg gcgactccat cggcgcgaaa
     8881 gtcaccgttg ttgccagtgg tgtccccgcc ggacttggcg agccggtctt tgaccgcctg
     8941 gatgccgaca tcgcccatgc gctgatgagc atcaacgcgg tgaaaggcgt ggaaattggc
     9001 gacggttttg acgtagtggc gctgcgcggc agccagaacc gcgacgaaat caccaaagac
     9061 ggtttccaga gcaaccatgc gggcggcatt ctcggcggta tcagcagcgg gcagcaaatc
     9121 attgcccata tggcgctgaa accgacctcc agcattaccg tgccggggcg cactattaac
     9181 cgctttggcg aagaagttga gatgatcacc aaaggccgtc acgatccctg tgtcgggatc
     9241 cgcgcggtgc cgatcgctga agcgatgctg gcgatcgttt taatggatca cctgctacgg
     9301 cagcgggcgc aaaatgccga tgtgaagact gatattccac gctggtaaaa aatgaacaaa
     9361 accgcgattg cgctgctggc tctgcttgcc agtagcgcta gcctggcagc gacgccgtgg
     9421 caaaaaataa cccaacctgt gccgggtagc gcacaatcga taggcagttt ttctaatggc
     9481 tgtattgtcg gcgctgacac gttgccgata cagtccgaac attatcaggt catgcgtacc
     9541 gatcagcgtc gctatttcgg tcacccggat ctggtgatgt ttatccagcg tctgagtagc
     9601 caggtgagca atctgggcat gggtacggtg ctgattggcg atatggggat gcccgctggt
     9661 gggcgtttca acggcggtca tgccagccac cggaccggac tggatgtcga tatctttctg
     9721 caactgccga aaactcgctg gacctccgcg cagctcttgc gcccgcaagc actggactta
     9781 gtttcccgcg acggtaaaca cgttgttccg acgctgtgga agccagaaat tttcagcttg
     9841 atcaaactcg ccgcccagga caaagacgtc acacgcattt ttgttaatcc ggcgattaaa
     9901 caacaacttt gcctagatgc gggcaccgat cgcgactggt tgcgcaaagt gcgaccctgg
     9961 ttccagcatc gtgcgcatat gcatgtacga ttacgttgtc ctgccgatag tctggagtgt
    10021 gaagatcaac ctttaccgcc accaggcgat ggttgcgggg cagaactgca aagctggttt
    10081 gcacctccaa aaccgggaac aacaaagcct gagaagaaga caccgcctcc gttgccgcct
    10141 tcctgccagg cgctactgga tgagcacgtg atctaatgga aacgtttaat agcctgttta
    10201 tggtttcccc gctgttgctg ggagttctct tttttgtcgc catgctggcg ggatttatcg
    10261 actcgattgc cggtggcggt gggttactca ccattccggc attgatggca gcggggatgt
    10321 ctcccgctaa tgcgctggca accaataaac tgcaagcctg cggcggctct atttccgcca
    10381 ctatctactt tattcgccgc aaagtggtta gtttaagcga tcagaaactc aatattgcca
    10441 tgacctttgt cggctcaatg agcggcgcac tgttggtaca gtacgttcag gctgatgtct
    10501 tacggcagat tttgcccatt ctggtgattt gtatcggcct ctatttcttg ctgatgccaa
    10561 agctgggtga agaagatcgc cagcgccgga tgtacggttt acccttcgcg ttaattgccg
    10621 gtggctgcgt cggtttttat gacggattct ttggcccggc tgccggatcg ttttacgcgc
    10681 tggccttcgt tacgctgtgc ggattcaacc tcgccaaagc cacggctcac gccaaattac
    10741 tcaacgcaac gtcaaacatt ggcggtctgc tgctgtttat tctcggcggc aaagtgattt
    10801 gggcgacggg ctttgtgatg ctggtggggc agttcctcgg cgcgcgcatg ggttcacggc
    10861 tggtgttgag caaaggacaa aagctgatcc gcccgatgat tgttatcgtc tcggcggtga
    10921 tgagtgccaa attactttac gatagccacg gacaggagat cctccactgg ttggggatga
    10981 actaatgaac agtacacacc actatgagca gttgattgaa atttttaata gctgctttgc
    11041 cgatgaattt aatacccgtc tgattaaagg cgacgacgaa ccgatctatc ttcctgctga
    11101 tgcggaagta ccgtataacc gaattgtctt tgcccacggc ttttatgcca gcgcaattca
    11161 tgagatttcg cactggtgta ttgccggaaa agcgcgccgt gagctggttg atttcggcta
    11221 ctggtattgc ccggatgggc gtgatgccca aacgcaaagc cagtttgaag atgttgaagt
    11281 gaagccgcag gcactcgact ggttgttctg tgtggcggcg ggatatccgt ttaacgtcag
    11341 ttgcgacaat ctggaaggcg acttcgaacc ggatcgtgtg gtgtttcaac ggcgggtaca
    11401 tgcgcaggtg atggattacc tagcaaacgg cattcctgaa cgcccggcac gcttcattaa
    11461 agcactacaa aattattatc acacgccgga acttacggcg gaacagttcc cgtggccgga
    11521 agcgctcaac taaagccagc gccacgtttt acaatgagga aagaagatga tcgcggagtt
    11581 tgaatcacgc attctggcat taatcgacgg tatggttgac catgccagtg atgatgagtt
    11641 gtttgccagt gggtatttgc gtggccacct gacattagcc atcgcagaac tggaaagtgg
    11701 tgatgaccac tccgctcagg cggtgcatac gaccgttagc cagagtctgg aaaaagccat
    11761 tggtgccggt gaattgtcgc cgcgtgacca ggcgctggtg accgatatgt gggaaaacct
    11821 gtttcagcag gcgtcacagc agtaagttgc ggcgtctgga gcaccatgcc tgatgcgacg
    11881 cttatcgcgt cttatcaggc ctacacgtca cacgtaggtc ggataaggcg ttcacgccgc
    11941 atccggcaaa cactgcgaga gcagattacc ccgccttaac cgctttaccc ttcaacaatt
    12001 tccgcaccca taaccgattc gggtttaacg ccgccagcgt actggcatcc atcggaatcg
    12061 gttcgtcgct catctgcgcc gccagaatct cggcacacag cggggcagaa cacaaaccgc
    12121 gagaacctaa agccgcaaac ataaagagat cgtcaaaaac cggcgcgctt accgcctcat
    12181 ctttctgctc cgccaacgac gcatattcca cgagtgttgc ctcataatcg ggaacattgc
    12241 ctaccattgg cagatgatcg cgggtggcac aacgcacacc gcagcgcgcc tctttatcac
    12301 tgacatcaac ctcttttgcc cactgtgcct gcgggaaaca atcaatcaac cgctggcgat
    12361 tctgctgctg atcgtcctca ctgtacgccg tatcttcgct gccgcgatga taactggcac
    12421 caatacaatg atgttgattc gccggatttt gtggcgtgag ataaccgtca tagcacagca
    12481 cctgcttcag ctctgccagt tccggcgttg tcggaatatg gctgacctgc ccggcaaccg
    12541 aatacaccgg gagagtcgac gtttggctga atcggctgat ttgatgcccg ttcgccagta
    12601 ccactacgct gtgtgttgct tgctgatctc ctgcaaaatt caacaaccaa cagtcatcct
    12661 tacgggataa attctgtaac tgatattgat aataaatctg caaaccctgc tgttgcgcca
    12721 gttccagcac attacgggtc agttctgctg ggcacagcca accaccttgc ggataagtaa
    12781 tgccgctgca atttgtcgca acgcccgtaa tttgttcaac cgcatttgcc tcaacggcta
    12841 cagccagttc tgcgggtaaa tccattgaca acatctgtgc gattttatgc tggcttttct
    12901 catcccagcc taactgcgtg acgccgcacc agtcatgatc aaatttaacg ggtaattggt
    12961 cgtaaaaccg acgagcaaaa gtaaacgcat tagagaaaaa gcggtttagc gcctcatcgt
    13021 gtttgcttaa taacggatac agcgccccct ggcgattgcc ggaagcaccc agtgcggggg
    13081 cctcatccgc gcaataaagc gttacctgcc agccgcgccg taatagcgcc agcgacaaca
    13141 acgcgctggc aataccaccg ccgataatcg ccgcttcccg tttgctgctg cccgtgcggt
    13201 taaaccacgg cgcggagcag gggagcggta atgtctgttc catcaccccg caaagcattt
    13261 cccgtttgcg cccaaagccc ttacgttttt gcatcgtgaa tccggcgtcc tgcaaaccgc
    13321 ggcggacaaa accggcagac gtaaatgtcg ccagcgtgcc gcccggacgc gccaactttg
    13381 ccatggcgtt aaacagattt tgcgtccaca tatccgggtt tttcgctggc gcaaagccgt
    13441 ccagaaacca ggcatctact ttttgattta gcgaatcgtc cagttggctg gtcagttcgt
    13501 taatatcgcc aaaccataaa tccagcgtca cgcggccttc atcgagcaat aaacgatggc
    13561 aaccgggcaa gggcattggc cactgcgcct gaagttgttc tgcccacgga gccagttccg
    13621 gccagtgttg atgcgctaag gctaaatccg cacgggtgag gggaaatttc tcaaaactaa
    13681 tgaaatgtaa gcgttgtaat tgcgcttgcg gatgcgcttc gcgaaactga tcaaatgcct
    13741 gccatagcgt caggaagttt aatccggtgc cgaagccgct ctctgctacc acaaacagag
    13801 gatgtggatg ctcaggaaag cgtacctcta attggttgcc tcccagaaaa acataacgcg
    13861 tctcttccag cccgttatcg ttggaaaaat agacatcgtc aaaatctcgg gaaacaggtg
    13921 taccctcagc attaaattcg aggttggcag gttgtatgga gtagtgtttc acgtaagtta
    13981 ctcgtcttac aggcggtggc tcgatcttag cgatgtgtgt aaggctgcgc aaatttctct
    14041 attaaatggc tgatcggact tgttcggcgt acaagtgtac gctattgtgc attcgaaact
    14101 tactctatgt gcgacttaca gaggtattga atgaaacgtg cagtgattac tggcctgggc
    14161 attgtttcca gcatcggtaa taaccagcag gaagtcctgg catctctgcg tgaaggacgt
    14221 tcagggatca ctttctctca ggagctgaag gattccggca tgcgtagcca cgtctggggc
    14281 aacgtaaaac tggataccac tggcctcatt gaccgcaaag ttgtgcgctt tatgagcgac
    14341 gcatccattt atgcattcct ttctatggag caggcaatcg cagatgcggg cctttctccg
    14401 gaagcttacc agaataaccc gcgcgttggc ctgattgcag gttctggcgg cggttccccg
    14461 cgtttccagg tgttcggcgc tgacgcaatg cgcggcccgc gcggcctgaa agcggttggc
    14521 ccgtatgtgg tcaccaaagc gatggcatcc ggcgtttctg cctgcctcgc cacgccgttt
    14581 aaaattcatg gcgttaacta ctccatcagc tccgcgtgtg cgacttccgc acactgtatc
    14641 ggtaacgcag tagagcagat ccaactgggc aaacaggaca tcgtgtttgc tggcggcggc
    14701 gaagagctgt gctgggaaat ggcctgcgaa ttcgacgcga tgggtgcgct gtctactaaa
    14761 tacaacgaca ccccggaaaa agcctcccgt acttacgacg ctcaccgtga cggtttcgtt
    14821 atcgctggcg gcggcggtat ggtagtggtt gaagagctgg aacacgcgct ggcgcgtggt
    14881 gctcacatct atgctgaaat cgttggctac ggcgcaacct ctgatggtgc agacatggtt
    14941 gctccgtctg gcgaaggcgc agtacgctgc atgaagatgg cgatgcacgg cgttgatacc
    15001 ccaatcgatt acctgaactc tcatggtact tcgactccgg ttggcgacgt gaaagaactg
    15061 gcagctatcc gtgaagtgtt cggcgataag agcccggcga tttctgcaac caaagccatg
    15121 accggtcact ctctgggcgc tgctggcgta caggaagcta tctactctct gctgatgctg
    15181 gaacacggct ttatcgcccc gagcatcaac attgaagagc tggacgagca ggctgcaggt
    15241 ctgaacatcg tgaccgaaac gaccgatcgc gaactgacca ccgttatgtc taacagcttc
    15301 ggcttcggcg gcaccaacgc cacgctggta atgcgcaagc tgaaagatta attcgcagta
    15361 ggccggagta gtcggagtag tcggagtagg tcggataaga cgcgccagcg tcgcatccga
    15421 cgttacgcgc taatgcggcc tccggcacta acgcaaaagg gaacctgatg gttccctttt
    15481 tcacatcatt gacaatcgcc gcctgttcca ggcaaacttc ccgctttgtc gatttccttc
    15541 tgaaaagacg tacgcgttaa atcctgccaa cgcactgtaa ccctgaaacc agagagatga
    15601 gacggggata ctcctcgcct tgcgctgcat tctggagtaa tgcatgactg ctgtaagcca
    15661 aaccgaaaca cgatcttctg ccaatttttc gctcttccgc atcgcttttg cggtttttct
    15721 cacctacatg accgtagggt tgccgttgcc ggttatcccg ctgtttgttc atcatgaact
    15781 gggctatggc aataccatgg tcggcattgc cgtcgggatt cagtttctgg ctacggtgct
    15841 gacgcgtggt tacgccgggc gactggccga tcaatatggt gcaaaacgtt cggcgcttca
    15901 gggaatgtta gcttgtggtc tggctggcgg cgcgttgctg ctggcggcga ttttgcctgt
    15961 ctccgcaccg ttcaaatttg ctctgttggt cgtcgggcgt ttgattcttg gctttggtga
    16021 aagccagtta ctgacaggcg ctctgacctg gggactaggc atcgtagggc caaaacactc
    16081 tggcaaagtg atgtcatgga acggaatggc gatttacggt gccctcgctg ttggtgctcc
    16141 gcttggcctg ttgattcata gccattacgg ttttgccgca ctggcgatca ccacaatggt
    16201 attacccgta ctggcgtggg cctgtaacgg cacagtgcgc aaagtaccgg ccctggcggg
    16261 agaacgtcca tcgctgtgga gcgttgtcgg gcttatctgg aaaccagggt taggtctggc
    16321 actacaaggc gttggttttg ctgttatcgg gactttcgtt tcgctctact ttgccagcaa
    16381 aggatgggcg atggcgggct ttactcttac cgcgtttggc ggcgcatttg tcgtgatgcg
    16441 cgtcatgttt ggctggatgc cggaccgttt tggcggcgtg aaagtggcga ttgtctctct
    16501 gcttgtagaa acggtgggct tgttgctgct ctggcaagcc ccaggtgcat gggtcgcatt
    16561 agcgggcgcg gcgttaaccg gagccggatg ttcgcttatc tttcctgcgc tgggcgtgga
    16621 ggtggttaaa cgcgtcccct cacaagttcg cggcaccgca ctgggcggtt acgccgcgtt
    16681 tcaggatatc gccctcggcg tctccgggcc gctggcggga atgctggcga ccacgtttgg
    16741 ttactcttcg gtatttcttg ccggggcgat ctctgcggtg ctgggtatta ttgtcacgat
    16801 actgtcattt cgtcggggtt aacgaaccag ccagaccagc atcaggataa tcgcgactaa
    16861 cagtatccac agcgcgggac gcgttgccat attttgcaac gtgtccatca tcggtggaaa
    16921 aggattaagc agcggttgca taacacgcgg atcaatccct ttaacccgcc gttgataaag
    16981 ctgattaaga atatcctcgg cctgggcaga agaaagcgat gattgcgcag aaagaccata
    17041 tttttgctgg atatatgccg ataacgccgc cagttcactg gcatctaaag gttgttttag
    17101 cgtcatctgt agtgattcca gcgtcggcgt attttgctgg cttagcgtct gacgcgcctg
    17161 tagccaggtc accagatggt taaacagttt cgctggaatt aactcgccat ctttcacccc
    17221 ggaaagttcc agcatcgatt gccagatctg tttgctgggt tcccccgttg ccgccgcaag
    17281 tttggtcacc agctgtttta gcgcattgtg ctccgccggt aataaaggac ggtcggtcgc
    17341 ctcgcgctgc tgtggttgcg gaataaccat cttcccttcc tgcaacaggg tgagaatggt
    17401 ttttaattgc tccggtgaga gctgattcag cggcgtctga ccaaagttat gacggatata
    17461 atccgtgacc gcctgacgat tattccccag acgtaaatac tcccctaatt gcgctaaaag
    17521 ctggcgggca gaatggcttt tttgcgcggc cagcagacgt tgcgccaggt tatgctcagc
    17581 ggcagggaag tgacgcgaaa gcagcggtga atctcccgac agaccaatat cgtgcctgat
    17641 acccgcccac agttctgctc tttgctgttg cgtcagtgag gtcactttcg tcattaagct
    17701 ttccagcgaa gtacgttgct gactggataa aggctgattg cccgcgccag acggcaggtt
    17761 atctccctga cctggtggtt gcccaggagg agggccggaa ataggttgta tcattacgta
    17821 tccttatacc tgaaatcttc gcaagtatgc ctggccgcga gattatggca cacttgtccg
    17881 gttaactctc gtctcataca ggtaacacaa acgtgaaaat ccttgttgat gaaaaaaatg
    17941 atgatttaat ttattgatat aaaaggattt atatcagcac gtgtccacgt agtgaccaca
    18001 ttttcgagtt acagataaca caaagcccgt tttcacgggc tttgtgttat ctgtgattgt
    18061 ggctaagcca catgatttat gctatttcca gttacaagca gcttgaagtg gttttagcgc
    18121 gtttgacagg ccgtttaaat tgaaagttgc acttacaggg ctctcattat aaggagttat
    18181 ttttgtatac attttgtttg agttggctag ttttcggatg aaatcaatgt cattaccttt
    18241 ataaaaaaca gcctttgtat ctgtagatac tagccagttt cgctcaactg ctttctgttt
    18301 atcaagacga taaagcatgc tggtttgttc aaggcctaaa tatacatccc aactaagaaa
    18361 tacttcggtt ttcttttctc ggcaagctat aaacagagtg ggagtaaccg cttctccaaa
    18421 tggagttctg atataatcat cactttctaa cgataaaatt acatttttcg aatcatcaat
    18481 tggcgatgta gtaatggatg tgtgccattt tcctacatct gtggctttct ctaattttga
    18541 ttcagcttga acagctttat cctgtgggaa tagtttatcg tagcagttta gtctggcttc
    18601 tttatcattt tctgctcggc atttagatat ctcagaaaga tttggttgct gagcctctgt
    18661 tgttactggc ttttccgttt tagattttga gtttacagat ggagtaaatg ttggagaaaa
    18721 aaaacgatca taacaagata aacgtctgtc atcatctttc tcatcagggc attgatctct
    18781 gctttcgaat ttttcagcaa ctttcgttcg agtcggttgt attgacttat cgtaacaaga
    18841 aagtctgtct ttattatttt ctattgctcg gcactggagt actgcattga agttattatg
    18901 ttcagttgct gcgtgaacag gcagtacact aattgatata aatgatgcac ctatagaaaa
    18961 aattatattt ttcattttgt tatccttttg gtttagtaag aaa
//
LOCUS       DAZFFI010000086        18760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFFI010000086 DAZFFI010000000
VERSION     DAZFFI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.086"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            312..1004
                     /gene="yieP"
                     /locus_tag="ACHJ12_003536"
     CDS             312..1004
                     /gene="yieP"
                     /locus_tag="ACHJ12_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4991270.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            1027..2454
                     /gene="mdtD"
                     /locus_tag="ACHJ12_003537"
     CDS             1027..2454
                     /gene="mdtD"
                     /locus_tag="ACHJ12_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4991271.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2420..3412)
                     /gene="rbsR"
                     /locus_tag="ACHJ12_003538"
     CDS             complement(2420..3412)
                     /gene="rbsR"
                     /locus_tag="ACHJ12_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4991272.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3416..4345)
                     /gene="rbsK"
                     /locus_tag="ACHJ12_003539"
     CDS             complement(3416..4345)
                     /gene="rbsK"
                     /locus_tag="ACHJ12_003539"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4991273.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4471..5361)
                     /gene="rbsB"
                     /locus_tag="ACHJ12_003540"
     CDS             complement(4471..5361)
                     /gene="rbsB"
                     /locus_tag="ACHJ12_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4991274.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5386..6351)
                     /gene="rbsC"
                     /locus_tag="ACHJ12_003541"
     CDS             complement(5386..6351)
                     /gene="rbsC"
                     /locus_tag="ACHJ12_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4991275.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6356..7861)
                     /gene="rbsA"
                     /locus_tag="ACHJ12_003542"
     CDS             complement(6356..7861)
                     /gene="rbsA"
                     /locus_tag="ACHJ12_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4991276.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7869..8288)
                     /gene="rbsD"
                     /locus_tag="ACHJ12_003543"
     CDS             complement(7869..8288)
                     /gene="rbsD"
                     /locus_tag="ACHJ12_003543"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4991277.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8455..10323)
                     /gene="kup"
                     /locus_tag="ACHJ12_003544"
     CDS             complement(8455..10323)
                     /gene="kup"
                     /locus_tag="ACHJ12_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4991278.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10546..12042
                     /gene="ravA"
                     /locus_tag="ACHJ12_003545"
     CDS             10546..12042
                     /gene="ravA"
                     /locus_tag="ACHJ12_003545"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4991279.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12036..13487
                     /gene="viaA"
                     /locus_tag="ACHJ12_003546"
     CDS             12036..13487
                     /gene="viaA"
                     /locus_tag="ACHJ12_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4991280.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13492..14484)
                     /gene="asnA"
                     /locus_tag="ACHJ12_003547"
     CDS             complement(13492..14484)
                     /gene="asnA"
                     /locus_tag="ACHJ12_003547"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4991281.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            14636..15094
                     /gene="asnC"
                     /locus_tag="ACHJ12_003548"
     CDS             14636..15094
                     /gene="asnC"
                     /locus_tag="ACHJ12_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4991282.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15184..15627
                     /gene="mioC"
                     /locus_tag="ACHJ12_003549"
     CDS             15184..15627
                     /gene="mioC"
                     /locus_tag="ACHJ12_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4991283.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16006..17895
                     /gene="mnmG"
                     /locus_tag="ACHJ12_003550"
     CDS             16006..17895
                     /gene="mnmG"
                     /locus_tag="ACHJ12_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4991284.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17959..18582
                     /gene="rsmG"
                     /locus_tag="ACHJ12_003551"
     CDS             17959..18582
                     /gene="rsmG"
                     /locus_tag="ACHJ12_003551"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4991285.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4710 a         4882 c         4631 g         4537 t
ORIGIN      
        1 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcattttt tgcttttctc
       61 tgttgagatt ctcaggagaa ccccgctgac ccggcaacgt gtttgccgtt gttccgtgtc
      121 agtggtggcg cattataggg agttattccg gcctgacaag aggaaattta aaataatttt
      181 ctgaccgcgc attttttcag caaaaccccc gttaatgtgc cataaaacaa gcaatttgcc
      241 gtttttgcaa ccgtaatcac acttcctggt ggcatactaa ggaggaataa aaagaaaaag
      301 gaagattagt aatgccttta agcgcacaac agttagctgc acagaaaaac ctttcgtatg
      361 ttctggctga gaagctggcg caacgcatct taaaaggtga atatgaaccc ggcaccattt
      421 tgcctggtga gattgagctg ggcgagcaat ttggagtgag tcgtacagcg gtacgcgaag
      481 cggtcaaaac gttaacggca aaagggatgg ttttaccgcg accgcgaatt ggtacccggg
      541 tcatgccaca atcaaactgg aattttcttg atcaggaatt gcttacctgg tggatgacag
      601 aagagaactt tcatcaggtc atcgatcact ttctggttat gcgtatctgt ctggagccgc
      661 aagcttgcct actggctgca acggttggca ccgcggaaca gaaggcgcat ctcaatacgt
      721 taatggccga aatggcggca ttaaaagaga actttcgccg tgaacgctgg atcgaagtcg
      781 atatggcctg gcatgagcat atctatgaaa tgagcgccaa tccgtttttg acctcattcg
      841 cctcgctatt ccattcggtt tatcacactt acttcacgtc aattaccagc gacacagtga
      901 taaagctgga tctgcaccag gcgattgtcg atgcgattgt ccaaagcgat ggcgacgcgg
      961 catttaaagc ttgtcaggcg ctgctacgct cacctgataa gtgacaaccc gataacagga
     1021 cattgaatga gcgataaaaa gaatcgcagt atggcgggtt tgccgtggat cgcggcgatg
     1081 gccttcttca tgcaggcact tgatgccact attctgaata ccgccttacc cgcaatcgct
     1141 catagcctta atcgttctcc tctcgcgatg caatcagcca tcatcagtta tacgctgacg
     1201 gtggcgatgc ttattccggt aagcggatgg ctagctgatc gcttcggtac gcgtcgcatt
     1261 tttacccttg ccgtgagtct gttcacattg ggttctctgg cctgcgcact ttctaattcg
     1321 ctaccacagc tggttgtctt ccgggttatt caggggatag gcggcgcaat gatgatgcct
     1381 gttgctcggc tggccttact gcgcgcttat cctcgtaatg aacttcttcc agtattgaat
     1441 tttgtcgcca tgccgggtct ggtggggcca attttaggcc ccgttcttgg cggcgtgctg
     1501 gtcacctggg caacctggca ctggatattt ttaatcaata tccccatagg tattgcgggc
     1561 cttctttacg cgcgcaaaca tatgcccaat ttcaccaccg cacgacgcag attcgatatc
     1621 actggctttt tgctgtttgg cctcagcctt gttctcttct caagcggaat agagctattc
     1681 ggggaaaaga ttgtcgccag ctggattgcc ttgacggtaa ttgtcaccag catcgggtta
     1741 ctgcttctct atattctcca tgcgcgacac acgccaaacc cattaatttc attagattta
     1801 tttaaaaccc gcactttctc gatcggtatc gtaggcaata ttgcaacccg tctggggacc
     1861 ggttgtgtac cgttccttat gccattgatg ttacaggtag gatttggtta tcaggcgttt
     1921 attgccggct gtatgatggc accgacagcg ttaggttcta ttattgcaaa atcgatggtt
     1981 acccaagtct tacgtcgtct gggctatcgc catacgttag tggggatcac ggtgattatt
     2041 gggctaatga tcgctcagtt ctctttgcaa tcaccggcaa tggcgatatg gatgctgatc
     2101 ttgccgttgt ttatattagg gatggctatg tcgacgcaat ttaccgcgat gaataccatc
     2161 acacttgccg atctgaccga tgacaacgcc agcagcggta acagtgttct ggcggtcacg
     2221 cagcaactgt cgattagttt aggcgttgct gtaagtgcgg ccgtccttcg cgtttatgaa
     2281 gggatggaag gcacaacgac tgtcgaacaa ttccactata cgtttatcac gatgggcatt
     2341 attactgttg cttcagcagc aatgttcatg cttctgaaaa caaccgatgg taataatttg
     2401 atcaaaagac agcgtaaatc taagccgaac cgcgttccat cagaatcgga gtaagttgta
     2461 atcgttgttg ctgaagggtc ggctgggtta tccgatggat gagtacatca atcgccagct
     2521 cccccagttc atctttcggt tggtggatag tggttaatgg tggcgtcata aagcttgcca
     2581 gttcgatatc gtcatagcca atcaccgcga tatcctgcgg aacctgtaac tctgcctgat
     2641 ataacgcctg gtaaacgcca acagccatag cgtcatttcc ggtaaagacg gcctgaggac
     2701 gcagcggatg tgataacagt tggcgcatgg cgtcaaaccc gccgttaaat tcaaaatcac
     2761 cagtgacttc atagccatca ggaatgttga gacccgcacg tttcatcgcc gcccgataac
     2821 cttccaaccg caggcgcgcc ggagttttat ccagcggacc ggtaatacag gcgatacggg
     2881 tatgaccttt atcgatcaga tattgcgttg ctaagtctcc gcccagcaac gagttatcct
     2941 gaataagatc gctgtcgcca tcgaacggag cccagtccat catcacagta ggcactgtcg
     3001 gataacgttg catgatttca cgcgaaggtt gatgcgtttc ggtgcacagt aacagcaagc
     3061 catcaacgcg tttttgcatc agcgtttcca gattgcgatt catccgctgt tcatctcctt
     3121 cggtattgca aaggacgaga ctataaccgc gttcgaagca gctgcgttca acgccacgca
     3181 ccagttctga atagaaagga ttggtactgg cagtgatcaa catgccaatg gtatgtgttt
     3241 gattgagttt gaggctacgc gccagggctg atggcgcgta attgagttct ttaatcgccg
     3301 cttcaacttt ggcggtaatc gcttcactga cgaagcgatc tttattgata acgtgagaaa
     3361 ctgttgaggt agaaacgccc gccaggcggg caacatcttt cattgtagcc aagcgtcacc
     3421 tctgcctgtc taaaaatgcg tcgatctctt cacgccacgg tacggaaggt tgtgcgcctt
     3481 tacgtgttac ggcaatcgca gcggcagcat gggcaaaccg aatcgcctct ggcaatggtt
     3541 tttcttccag caacgccgtg attaacgcgc cgttaaaggt atctccggca gcaatggtat
     3601 cgacagcctg cacccggaat ccaggaacgc gctgaccttc accattcacg ctagcccata
     3661 caccacgact tcctaaagta atcagtacag tacggatacc tttttcatgc agtacctgcg
     3721 ccgccttcgc tgcatcttca tcattttcaa cacgaatacc ggtgagcttt tctgcttccg
     3781 tttcgtttgg cgtaataatg tccaccagcg ccagcagttc gtcaggaagt tcgcgagccg
     3841 gagccgggtt aagcgcaacg atagtcttat tttgatgggc gattttcgcc gctgccatca
     3901 cactttcgag tggtgattcc agctgcatta ataatgctga cgcgttggca atacgctcac
     3961 gttgcgcttc caccagcgcc ggggaaaggg cagcattagc gccggcatga ataccgatga
     4021 cattctcacc ttcgccatta acaaaaatca gcgccacacc tgttgattcg cctttgatca
     4081 cgctgaccgg agtaatatca atgttatcag tggcgagctg ctggcgaacg ctctcaccaa
     4141 tgctgtcatc acccgtacag gcaataaacg cgatattcgc accgctacgc ccagcagcca
     4201 cagcctgatt cgcgcctttg ccgccaaatg caacctgata gtggttaccg gttacggttt
     4261 cgcctggagt aggaaaagat tgaagattaa gaatgtggtc agcattaatg ctgccaagaa
     4321 caacgaggct gcctgcgttt tgcatattcg ggatgtccat aaaatgcgcc accgtgttag
     4381 ggtggcgcgt gccctgcttt tctttatatt acacgttcat ctgtcgatga cgtatttatg
     4441 tcaccatcag gtcatacaac ctgattaaaa ctactgctta acaaccagtt tcagatcaac
     4501 cggatactta gcctgaactt tctcgccttt cagcacttta tctgcggttt cgacgccttt
     4561 cgcgccaatc tgatcgggta gctgagcgat agtcgctgct agtttgccat cattcaccgc
     4621 tttttcgcca tccggtgtac cgtcaaatcc gacgaccatc acatccgatt taccggcagt
     4681 ttgcagtgcg cgcagcgcgc ccagcgccat ttcatcattc tgcgcgaata cagcctgaac
     4741 atccggatga gcggtcaaca ggttctgcat tacgttcaaa cctttagtgc gatcaaaatc
     4801 tgctggctgg ctggcaagaa cattaaactt gtgagcagca acggcctgct ggaagccttc
     4861 gccacgttca cgggctgcgg atgtaccagc aatgccttgc agctcgataa ctttggcacc
     4921 ttcacccgct ttcttcgcga tgtaatcacc ggcgattttg ccacccagta cgttatcaga
     4981 agcaatgtgg ctcaccactt cacctttcgt tgcctggcgg tcaagagtga taaccgggat
     5041 gttcgcctgg ttagccatct tcacagcatt acctactgcg tcggagtcgg tcgggttaat
     5101 cagcagaatt ttggtgccgc gaacggttaa gtcctgcacg ttcgccagct ctttcgccgg
     5161 gttgttctgg gagtccagca ccaccaggtt atagccaagc ttatccgcct ctttctgcgc
     5221 gccatctttc agcgatacaa agaacgggtt gttaagcgtg gagaccacca gcgcgatggt
     5281 gtcttttgcc atcgcattcg cactgacggt ggcgcttagc gcaacagcgg aaaccagggt
     5341 agccagtttt ttcatgttca tattcaagat gtcctgtagt cgttattact gctttttgtt
     5401 gtctaccagc accgccagca aaatcaccac cgctttgacg atcatctggt aataggagga
     5461 aacacctaac aaattcaatc cattattaag gaagccaaga attaatgcgc cgatcaacgt
     5521 cccaacaatg cgaccttttc cgcccgccag actcgtaccg cccagaacca ccgcagcaat
     5581 agcatccagc tcatagccag tccccgccgt gggttgtgcg gaggagagac gcgccacttc
     5641 aatgatcccg gccagcgatg ccagcagacc acaaagagaa tagacgatga ttttgatttt
     5701 attgacgttg ataccagaaa gacgcgttgc cgcttcgttg ccgcccagcg cgtagatgta
     5761 acgccccaga cgcgtgtgat gcagcatgta ccaggccgcg aggaagacaa tccccatgat
     5821 ccagactggc gtcggtacgc ccagcggacg accaatacca aaccagccaa acagatcggc
     5881 gttctcagta aagccggtat tcactgggct accgttggta taaaccatgg tcacgccgcg
     5941 cagtaaaagc atcataacca gcgtagcgat aaacgcctga acgcgacctt tcgctacaat
     6001 caccccggtt accgcaccaa ttgcggcacc taacgcgaga gcggcagcga cagccaccag
     6061 cgcattgact tcaatgccga cgatagatgc agcaactgcg ccggttagcg ccaacagaga
     6121 acctacagac aagtcgatgc ccgacgtcag gatcaccagc gtcatcccga ccgccataat
     6181 ggcgttcacc gaggtttgct ggagaatatt gaataagtta ttgatggtga aaaagttcgg
     6241 gcttaacgtc gagacaatcg cgatcagcac cagcagagcg ataagcgatt tctgctccat
     6301 cagccacgct ttcgtgaaat aacggcgacc agagacagtc tgggttgtca tttttttact
     6361 cctgattcac gcgattaagc ttgcccacag cggcagccat taacacttcc tgggtggcct
     6421 gctcacgagt aaattccccg ctgagatgcc cttcatgcat gacgatgatg cgatcgctca
     6481 tgcctaatac ttctggcatc tccgatgaca ccagaatgat gctcaagcca tcggctttga
     6541 actggttaat cagttgatag atctcttttt tcgcgccgac atctacgcca cgggtaggtt
     6601 catcaaggat caacactttg gggcgtgtca tcagaccgcg ggcaatcgcc actttttgct
     6661 gattgccacc ggaaagcaga ccaattgcct gttccatcga cggtgttttc acattaaaca
     6721 gacgaatgaa atcactcaca gcctgctgtt catcggcatg cttcaaactg ccgccagcgc
     6781 ggctgaagta gcgcagcgct gtcagcgaca tgttctcttt tactgacatg cccaacacta
     6841 aaccgtcacg tttacggtct tcggagatat acacaatgcc gcttgccagg ccatcctgcg
     6901 gtgaacgggt aacgacttca tgcccatcca gggtgacgta accgctggtg cgcggtagtg
     6961 cgccgtagag cactttcatc agttcggtac gacctgcgcc catcaaacca gagacgccaa
     7021 gaatttcgcc tttgcgtaaa gtaaaagaga catcgttaac gccaggtccg cagagattat
     7081 cgactttcag gcggatatct cccggcgctt tgtccaggtg tggatattga tcttctagct
     7141 tgcggcccac catcatctca atcagcgaat cttcggtcag tgatgccact tcgcgctcag
     7201 caataaattg cccgtcacga aaaacggtaa cgtcatcgca aatctcgaag atttctttca
     7261 tgcggtggga gatatagaca ataccgcggc cttgcgattt cagctcgcgt atgacgcgga
     7321 acagggattc ggtttcggta tcggtcagcg catcggtcgg ttcatccata atgatgactt
     7381 tcgactcaaa gctcagcact ttggcgattt caaccatttg ctggtcaccg atggaaagat
     7441 cgcccaccag cttgtcgctt ttaaagcgca ggttaagttt agccagcaat ttatccgctt
     7501 cggcatacat ggttttccag tcaattttgc caaagcgatt aacaaactca cgaccgagga
     7561 aaatgttttc ggcaatggtc aactgcggga tcaggttcag ttcctgatgg ataatcccaa
     7621 tcccggcttc ctgggaagat ttcggcccgg taaatgtcgt ttctttcccc agccataaaa
     7681 gcgtgccggc atcgcgagca tagataccag taagcacttt catcatggtg gatttacccg
     7741 cgccgttttc gcccaccagc gccatcacgc ggcccggata gacatttaac gctgcgcccg
     7801 agagggcttt tacgcccggg aaggctttat cgatgccttt aagctgaagt aatgcttcca
     7861 tgacggcctc agaacgtcac gccagcacag agaatgatat tcgcatacgg agaacattct
     7921 ccgctgcgaa ttaccgcctg actttctgcg gtttgttgtt tgaattgttc atgcgtggtg
     7981 taacgaattt caatggtatt tccctggtgt ttttgcagct gctcaaggtg agtgagcaac
     8041 gtttcgtgga gttgcggatt atgttgtttg atctcttccg cgataatggc agcctcgacc
     8101 tgcatttcat ttgtgacgac gcccagcacc tgcataaaag aaggtacacc ctgggttaat
     8161 gccatatcga tacgcgttgt acttttgggg atgggtaaac cagcatcaca caccaccagc
     8221 gtatcggtat gtcccagacg ggagatcacc gatgaaatat cagaattaag aacggtgcct
     8281 tttttcattt ttttctccat cagcgaaacg tttcgctgac ccgcagttta atcctgactg
     8341 agttgggaaa accatcgtga cgttacggaa atgtgatcaa cctcgaaacg ttttacatgg
     8401 tgattaacca tgaaaacaaa aacgccccct tatggagaaa ggaggcgtct ggcgttagat
     8461 ttcgacctga gtacccagtt cgataaccct gtttggcgga atttcaaatt gatctggtgc
     8521 acgcagcgca ttacgttgca gcagcaagta cagcttgccg cgcagacgca aataccacgg
     8581 gcgtttgccg aggatcaacg actcatgcga cataaagaag gaggtttcca tcatccggca
     8641 acttaatcct tccagaccgc agcggtggaa aacttcttcc acgtttggcg tttctcgcca
     8701 accataactt gccaccacgc gccagaaagt gggcgacagt tgttcaatct gtacccgacg
     8761 gacattatgg acatatggcg cgtcttcggt acgcagagtt aacagaatca cccgctcatg
     8821 caataccttg ttatgtttaa ggttatgcat cagcgcaaag ggaatgacgt tgattgcacg
     8881 cgacatatac accgcggtcc cgggcacgcg aacaggcggt gatttctcca gcgaagcaat
     8941 catcgcttcc agagagttac catgttcatg catccgccgc agcaagcgga aacgctcgct
     9001 cttccaggtg gtcatcacga taaacatcac agtaccgagg ctcaatggca accagccgcc
     9061 ggagagcagt ttatcgaggt tagcggtgaa caatggaata tcgacacaaa ggaaagcaat
     9121 caggatcagc gcaacaaaat acttattcca gtgccagttc tgacgtgcca cggtagtcga
     9181 gagaatagac gtcagcacca tggttccggt caccgcaatc ccgtacgccg ccgccaggtt
     9241 gctggagtgc tcaaagctga caatcacaat cacgaccgcg acatagagca tccagttcac
     9301 aaacggaata tagatttgcc ctgactccat ttcggaggtg tgaataatgc gcatcggcga
     9361 caaatatccc agacgtaccg cctgacgcgt caatgagaag acgccagaga taaccgcctg
     9421 cgaggcaatt accgtcgcca gtgcggcgat gatcagcagc gggatcagcg cccagtccgg
     9481 tgccaacagg aagaacgggt tcttaatcgc ttccgggttc tttaacaaca gcgctccctg
     9541 accgaagtaa ttaagggtca aggaaggtaa tacgacggtg aaccacgcca ggcgaatagg
     9601 gaacttacca aagtgcccca tatcagcata cagcgcctcg acccccgtaa tcgacaacac
     9661 cactgcccct aatgcaataa aagaaaccgt tttgtattca aggaagaaat gcaccgccca
     9721 cattggattc agcgcatgca gcacttccgg gttagcaata atgctacgta accccagtcc
     9781 tgccagaatc aaaaaccagg tcagcatgat cggcgcaaac agcttaccga ccatagccgt
     9841 gccatgtttt tgaatcataa ataataaagt gagaacgata attgagaggg gaactatcca
     9901 ggtatccagc tgcggggcga cgatttccag accttcaatg gctgacatca ccgaaatagc
     9961 gggtgttatg acgacttcac catagaaaaa gctgccgccg attagcccca taatcaccag
    10021 cattgatgtg gttcgcgccg acgtattacg cccggcaagc gacatcaacg tcaggatccc
    10081 cccctcaccg gcgttatctg cccgcatcac gaaggtgaga tatttaatgg aaaccacaaa
    10141 gattagcagc cagaagatca gcgataaaaa gccaaacacg gcatcgcgtt caacgccaaa
    10201 accaaactgg ccggacaaac attcacgaag tgtatataac gggctggtac caatatcgcc
    10261 gtagacaact ccaatcgccg cgagggtaat cgcgggcaat gattgcttat tatcagtgct
    10321 catagactag tctttcgttg aaatatgaaa tgtgtgctta gtcccttggc ccacaaaaaa
    10381 gcgcacagta tgcacgatta acggcaaaat cgtactccta aatgcggcca cattaacctg
    10441 gctcaaagaa aaataccccc cctttgagac tattttagcc accagcaaga aacgtctata
    10501 ctcgcaattt acgcagaact tttgacgaaa ggacgccact tcattatggc tcaccctcat
    10561 ttattagcgg aaagaatttc ccgcctgagc agttcgttgg aaaaggggct ttatgaacgt
    10621 agccacgcca tccgcttgtg tttattagcg gcattaagtg gtgaaagtgt gttcctcctt
    10681 ggcccgccag gtattgccaa aagtttgatc gcccggcgct taaaattcgc ctttcagaat
    10741 gcccgcgcgt ttgaatatct gatgacccgc ttctccacgc cggaagaagt ttttggtccc
    10801 ctttctattc aggcgctaaa agatgaaggg cgctatgaac gtttaaccag cggttacctg
    10861 ccggaagcag aaattgtctt tctggatgag atctggaaag cgggcccggc aattcttaat
    10921 accttgctca ccgccattaa cgagcgccag ttccgcaacg gtgcacacgt agaaaaaatc
    10981 ccgatgcgtc tgctggtggc ggcctccaac gagctgccgg aagcagacag cagtctggaa
    11041 gcgttatatg accgcatgct gattcgtctg tggttagata aagtgcagga taaagcgaat
    11101 ttccgctcca tgctgaccag tcaacaggat gaaaacgaca atccggttcc tgatgccttg
    11161 caggtcacag atgaagaata tgaacgctgg cagaaagaga ttggtgaaat tacgctgccc
    11221 gatcatgtat ttgagctgat ttttatgctg cgccagcaac tggataaatt accggatgcg
    11281 ccttatgtct cggatcgtcg ctggaaaaaa gcgatccgct tattgcaggc cagcgccttt
    11341 tttagcggtc gcagtgctgt tgccccggta gatctcattt tgctgaaaga ttgcctgtgg
    11401 tatgacgcgc aaagcctgaa tttgatacaa caacaaattg atgtattgat gaccggtcac
    11461 gcctggcaac agcaagggat gttgactcgc cttggcgcga ttgtgcaacg tcacctgcaa
    11521 ctccagcagc aacaaagcga taaaacagcc ttaacggtaa ttcgtctggg cggcattttc
    11581 agccgtcgtc agcagtatca acttcctgtt aacgttactg cttccactct gactctgctg
    11641 ctgcaaaaac cgttaaaatt gcatgatatg gaagtggttc atatctcctt tgagcgtaac
    11701 gcgctggaac agtggctgag taaaggtggt gaaattcgcg gcaaactgaa cggtatcggc
    11761 tttgcccaga aattgaatct ggaagtggat agcgcccaac atcttgttgt acgcgatgta
    11821 agtttacaag gcagtacgct ggcacttccc ggttcatcgg ctgaaggtct gccaggtgaa
    11881 ataaaacaac aactggaaga gcttgaaagt gactggcgca agcaacacgc tttattcagc
    11941 gaacagcaaa aatgtctgtt tatccctggc gactggttag gtcgcattga agccagccta
    12001 caggatgtcg gcgcacagat tcgccaggca caacaatgct aacgctggat acgcttaatg
    12061 tgatgctggc cgtcagcgaa gagggattga tcgaagagat gatcatcgcg ctgctggcct
    12121 caccgcagct ggcagtcttc tttgaaaaat tcccacgact gaaggctgca atcactgatg
    12181 atgttccccg ctggcgtgag gcgctgcgca gtcggctgaa agatgcccga gtcccgccgg
    12241 aactcaccga agaggtgatg tgctatcagc aaagccagct cctctccacg ccgcagttta
    12301 ttgtgcagct accacagatc ctggacttac tgcatcgtct gaattcccca tgggcagaac
    12361 aagcccgaca gttggttgat gctaacagca cgatcacttc agcgttacat acgcttttcc
    12421 tccagcgttg gcgtttaagt ctgatcgtgc aagcaacgac gttaaatcaa cagctattag
    12481 aagaagaacg cgaacaactg ttaagtgaag ttcaggaacg catgacgctg agcggacaac
    12541 ttgaaccgat tctcgcagat aacaataccg cagctggtcg tctgtgggat atgagcgccg
    12601 gtcagcttaa acgtggcgac tatcagttga ttgtgaaata cggtgaattt cttaacgaac
    12661 agccggaact gaaacgcctg gcagaacagc tggggcgttc ccgggaagcc aaatcaatac
    12721 cgcgtaacga tgcgcagatg gaagccttcc gcaccatggt gcgcgaaccg gcaactgttc
    12781 ctgagcaggt tgatggtctg caacaaagcg atgatatttt acgtctcctg ccgccagaac
    12841 tggcgacact agggataacg gaactggagt atgagtttta ccgtcggctg gtggaaaaac
    12901 agttgctcac ctatcgcctg cacggtgagt cgtggcgtga aaaagtgatc gaacgtccgg
    12961 tggtacataa agattacgat gaacagccgc gcgggccgtt tattgtctgt gtggatactt
    13021 ccggctcaat gggcggcttt aatgaacagt gtgcgaaagc gttctgcctg gccttgatgc
    13081 gcattgctct cgctgaaaac cggcgctgct atattatgct attttccacc gagatcgtcc
    13141 gttatgagct ttcaggccca caaggcatcg aacaagcaat ccgtttttta agccagcagt
    13201 ttcgtggcgg caccgatctt gccagttgtt ttcgcgccat tatggaacgc ttgcaaagca
    13261 gggaatggtt tgatgccgac gcggtggtga tttctgattt tatcgctcag cggttgcctg
    13321 acgacgtgac gagtaaagtg aaagagctgc agcgggtaca tcagcatcgc tttcatgccg
    13381 tggcgatgtc ggcacacggc aaacccggca tcatgcgcat tttcgatcat atctggcgct
    13441 ttgataccgg gatgcgaagc cgcctgctca gacgctggcg gcgataaatt attacagcag
    13501 agaagggacg ttctcgcgaa cagcagctgg ccatacacca cactgaacct ggccgatatg
    13561 cggcagttgc agcagcagca tggtcaaacg agactggccg ataccgccgc cgatggtctg
    13621 cggcatttca ccgcgcagca gcgcctgatg ccactccagc tccagacgat cttcatcacc
    13681 ggtcagcgcc aactgatgct tcaacgtgtc ggcatccacg cggatcccca tggaagaaag
    13741 ctcaaacgca tcttccagta cagggttcca caccagaata tcgccgttca gacccgcatg
    13801 acccagctct gacggggtgc tccagtcatc ataatccggt gcgcgcacgt cgtggcgatg
    13861 accatcgctc agcttgccgc caatcccgac aaggaatacc gcgccaagat ctttcgctat
    13921 cgcccgctca cgccctttgg catcgagatc cggataacga gacagtaact cctggctgtg
    13981 tacgaagtgg atctgatccg gcaggaacgg tgccaggcca aactcttcgc taaccgcagc
    14041 ttcggttgct ttaattcccg cccagatcgc ctctaccgtg cttttcagag tcgagaattg
    14101 acgctcaccg tcgcccatta cgcgttccca gtcccactgg tcaacataga ccgagtgcaa
    14161 cggagaaaga cggtcttcat cggggcgaag ggctttcatg tgcgtgtaca gtccttcgcc
    14221 cgcgctgaag tcgtgttgcc ctaaggtctg acgtttccac ttcgccagtg aatgaaccac
    14281 ttcgaactgg gcatcaggca gagctttcac ttttacctgc accgcttttt cacagcccga
    14341 caagttatcc tgcgtgccat cccccacacg gctaagaatc ggcgcctgga cttcgatcag
    14401 ccccagacgt tcttccagtt ggcgagaaaa gtgagatttc acgaagctaa tttggcgttg
    14461 tttggcaatg taagcggttt tcatttttta tactcctgcg tcctgttgct tatgattaag
    14521 caacaaaaac cgacacacat gcaataatca ttcaataaaa agcctaacac acttttgatt
    14581 cattaaaaaa gaaggctaaa atagaatgaa tcatcaatct gcataagaaa atcctatgga
    14641 aaattatctg atcgacaatc tggaccgtgg catcctggaa gcattaatgg gcaatgcgcg
    14701 caccgcttac gccgaactgg cgaaacaatt tggcgtcagt ccggggacga ttcacgttcg
    14761 agtagagaaa atgaagcagg cggggatcat taccggggcg cgtattgatg tcagcccgaa
    14821 gcagctcggt tatgacgtag gctgctttat cggcattata ttaaagagcg ccaaagacta
    14881 cccttccgcg ctggcaaagc tggaaagcct tgatgaagtc actgaagcct attacacaac
    14941 cggccactac agcatcttta taaaagtgat gtgccgttcg atcgacgctc tccagcatgt
    15001 acttatcaac aagatccaaa caattgatga aattcagtcc accgagacat tgatcgtcct
    15061 gcaaaacccg atcatgcgta ccatcaagcc ataatcggct tttttaatcc catacttttc
    15121 cacaggtaga tcccaacgcg ttcacagcgt acaatacgcc actcttaata aaggtggcgg
    15181 tttatggcag atatcactct tatcagcggc agcaccctcg gcggtgccga atatgtagca
    15241 gaacacctgg ctgaaaagct ggaagaggcg ggttttacca ccgaaacgct gcacggtccg
    15301 ctgttggaag atttatctgc ctcggggatc tggctgatta tcagctccac ccacggtgcc
    15361 ggagatattc cggacaacct ttctcctttc tatgaagcat tgcaggaaca gaagcccgat
    15421 ctttctgcgg tccgctttgg cgcaatcggt attggcagtc gtgaatatga caccttttgt
    15481 ggggctatcg ataaactcga ggcagaactc aaaaattccg gtgcaaaaca gacaggcgaa
    15541 acactgaaga tcaacattct tgatcacgac attccggaag atccggcaga agaatggctg
    15601 ggatcgtgga ttaatttact caaataagta tacagatcgt gcgatctact gtggataact
    15661 ctgtcaggaa gcttggatca accggtagtt atccaaagaa caaccgttgt tcagtttttg
    15721 agttgtgtat aacccctcat tctgatccca gcttatacgg tccaggatca ccgatcattc
    15781 acagttaatg atcctttcca ggttgttgat cttaaaagcc ggatccttgt tatccacagg
    15841 gcagtgcgat cctaataaga gatcacaata gaacagatct ctaaataaat agatcttctt
    15901 tttaataccc aggatcccag gtctttctca agccgacaaa gttgagtaga atccacggcc
    15961 cgggcttcaa tccattttca taccgcgtta tgcgaggcaa tcaccatgtt ttatccggat
    16021 ccttttgacg tcatcatcat tggcgggggt catgcaggca ccgaggccgc gatggccgcg
    16081 gcgcgtatgg gtcaacagac tctgcttttg acacacaata tcgacactct ggggcagatg
    16141 agctgcaacc cggcgatcgg cggtattggg aagggacatc tggtaaaaga agtggatgca
    16201 ctcggcggtc tgatggcgaa agcgatcgat caagcgggta tccagtttag gatactaaac
    16261 gcaagcaaag gaccggcggt tcgcgctacc cgagctcagg cggatcgtgt gctctaccgt
    16321 caggcggtac gtacggcgct ggagaaccaa ccgaacctga tgatcttcca gcaggcggtt
    16381 gaagatctta ttgtcgaaaa cgatcgcgtg gtcggtgctg ttacccaaat gggactgaag
    16441 ttccgtgcca aagccgtcgt gctcaccgtt gggacgttcc tcgacggtaa aattcatatc
    16501 ggtctggata attacagcgg tggccgtgct ggtgatccgc cgtccattcc gctttctcgc
    16561 cgtttgcgtg aactgccgct gcgcgttggt cgtctgaaaa ccgggacacc accgcgtatt
    16621 gatgctcgaa ctatcgactt tagtgtgctg gcgcaacagc atggcgataa cccaatgccg
    16681 gtattctcgt ttatgggcaa tgcgtcccag catccccagc aggtgccgtg ttatatcact
    16741 cataccaacg agaaaaccca tgatgtgatc cgcagtaacc tcgatcgtag cccaatgtat
    16801 gcaggggtga tcgaaggtgt cggcccacgc tactgcccgt cgatcgaaga caaagtcatg
    16861 cgcttcgccg acagaaatca gcatcagatc ttccttgaac cggaaggact gacctctaat
    16921 gaaatttatc cgaacggtat ctccaccagc ctgccgttcg atgtgcagat gcaaatcgtc
    16981 cgctctatgc aggggatgga aaacgcgaag atcgtgcgtc cgggttatgc cattgagtat
    17041 gacttcttcg atcctcgcga cctgaaaccg acgctggaga gcaagtttat ccaggggctg
    17101 ttctttgctg gtcagattaa cggcactacc ggttacgaag aagccgctgc gcaaggtttg
    17161 ctggccggtc ttaacgctgc ccgtctgtct gctgacaaag aaggttgggc tccggcacgt
    17221 tctcaggcgt atctcggcgt actagttgat gacctgtgca ctttaggaac caaagaaccg
    17281 tatcgtatgt ttacttcgcg cgcagaatat cgtctgatgc tacgcgaaga taatgcggat
    17341 ctgcgtttga ctgaaatcgg tcgtgaactg ggcctggtgg atgacgaacg ttgggcgcgc
    17401 tttaacgaga aacttgagaa tatcgagcgt gagcgtcagc gtctgaaatc gacctgggta
    17461 accccgtcgg cggaagctgc agccgaagtg aatgctcacc tgactgcgcc gctttcccgt
    17521 gaagccagtg gtgaagatct gctgcgtcgt ccggaaatga cttatgaaaa attaaccacg
    17581 ctgacgccgt ttgcccctgc gttgacagac gaacaggcgg cggaacaggt tgagattcag
    17641 gttaaatacg aaggttatat cgcgcgccag caagatgaga tcgaaaagca gctgcgtaac
    17701 gagaacaccc tgctacccgc gacactggat taccgccagg tatccggtct ttctaacgaa
    17761 gtgatcgcca aacttaacga tcacaaacca gcctctatcg gccaggcttc gcgtatttct
    17821 ggcgtcacgc ctgcggccat ctccattctg ctggtgtggc tgaaaaaaca gggtatgctg
    17881 cgtcgtagcg cataacgcat taaaaatgcc tggtaagcac ccgcttacca ggcaacgcat
    17941 caagaacagg taatcaccgt gctcaacaaa ctctccttac tgctgaaaga cgcaggtatt
    18001 tcgcttaccg atcaccagaa aaaccagctt attgcctacg tgaatatgct gcataaatgg
    18061 aacaaagcgt acaacctgac ttcggtccgc gatcctaatg agatgctggt acgccatatt
    18121 ctcgatagca ttgtggtggc accgtatctg caaggtgaac ggtttatcga tgtcggcacc
    18181 ggaccaggac tgccaggcat tccactctct atcgtgcgtc ctgaagccca tttcactctg
    18241 ttggatagcc ttggtaaacg cgtgcgtttc cttcgtcagg tgcaacatga gcttaaactg
    18301 gagaatattg aaccagtaca gagcagggta gaagagtttc cttcagagcc gccatttgat
    18361 ggcgtaatta gccgcgcttt tgcctctctg aacgatatgg tgagctggtg ccaccatctt
    18421 cctggtgagc aaggccgttt ctacgcgctg aaagggcaaa tgccggaaga tgaaatcgct
    18481 ttgttgcccg aagaatatca ggtcgaatca gtggttaaac ttcaggttcc agccctggat
    18541 ggcgaacgtc atctggtggt gattaaagca aataaaattt aatttttatc aaaaaaatca
    18601 taaaaaattg accggttaga ctgttaacaa caaccaggtt ttctactgat ataactggtt
    18661 acatttaacg ccacgttcac tcttttgcat caacaagata acgtggcttt ttttggtaag
    18721 cagaaaataa gtcattagtg aaaatatcag tctgctaaaa
//
LOCUS       DAZFFI010000087        18105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFFI010000087 DAZFFI010000000
VERSION     DAZFFI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.087"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..1833)
                     /gene="lsrK"
                     /locus_tag="ACHJ12_003552"
     CDS             complement(241..1833)
                     /gene="lsrK"
                     /locus_tag="ACHJ12_003552"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO4991286.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1912..2865)
                     /gene="lsrR"
                     /locus_tag="ACHJ12_003553"
     CDS             complement(1912..2865)
                     /gene="lsrR"
                     /locus_tag="ACHJ12_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO4991287.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3114..4649
                     /gene="lsrA"
                     /locus_tag="ACHJ12_003554"
     CDS             3114..4649
                     /gene="lsrA"
                     /locus_tag="ACHJ12_003554"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO4991288.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4643..5671
                     /gene="lsrC"
                     /locus_tag="ACHJ12_003555"
     CDS             4643..5671
                     /gene="lsrC"
                     /locus_tag="ACHJ12_003555"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO4991289.1"
                     /translation="MLKFIQNNREITALLAVVLLFVFPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKELSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTSPPSVKPAS
                     SGKNREAA"
     gene            5671..6663
                     /gene="lsrD"
                     /locus_tag="ACHJ12_003556"
     CDS             5671..6663
                     /gene="lsrD"
                     /locus_tag="ACHJ12_003556"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO4991290.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6675..7697
                     /gene="lsrB"
                     /locus_tag="ACHJ12_003557"
     CDS             6675..7697
                     /gene="lsrB"
                     /locus_tag="ACHJ12_003557"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO4991291.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNRVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7724..8599
                     /gene="lsrF"
                     /locus_tag="ACHJ12_003558"
     CDS             7724..8599
                     /gene="lsrF"
                     /locus_tag="ACHJ12_003558"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO4991292.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8623..8913
                     /gene="lsrG"
                     /locus_tag="ACHJ12_003559"
     CDS             8623..8913
                     /gene="lsrG"
                     /locus_tag="ACHJ12_003559"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO4991293.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8970..9728
                     /gene="tam"
                     /locus_tag="ACHJ12_003560"
     CDS             8970..9728
                     /gene="tam"
                     /locus_tag="ACHJ12_003560"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO4991294.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENEQILLAFPRLFIVARRTE"
     gene            complement(9732..10646)
                     /locus_tag="ACHJ12_003561"
     CDS             complement(9732..10646)
                     /locus_tag="ACHJ12_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO4991295.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10853..12304)
                     /gene="uxaB"
                     /locus_tag="ACHJ12_003562"
     CDS             complement(10853..12304)
                     /gene="uxaB"
                     /locus_tag="ACHJ12_003562"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4991296.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12531..13949)
                     /locus_tag="ACHJ12_003563"
     CDS             complement(12531..13949)
                     /locus_tag="ACHJ12_003563"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4991297.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14088..14447)
                     /locus_tag="ACHJ12_003564"
     CDS             complement(14088..14447)
                     /locus_tag="ACHJ12_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4991298.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14447..15373)
                     /gene="glsB"
                     /locus_tag="ACHJ12_003565"
     CDS             complement(14447..15373)
                     /gene="glsB"
                     /locus_tag="ACHJ12_003565"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4991299.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15437..16825)
                     /gene="sad"
                     /locus_tag="ACHJ12_003566"
     CDS             complement(15437..16825)
                     /gene="sad"
                     /locus_tag="ACHJ12_003566"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4991300.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINSYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16926..17807
                     /locus_tag="ACHJ12_003567"
     CDS             16926..17807
                     /locus_tag="ACHJ12_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4991301.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17884..>18105
                     /gene="yneK"
                     /locus_tag="ACHJ12_003568"
     CDS             17884..>18105
                     /gene="yneK"
                     /locus_tag="ACHJ12_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO4991302.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSF"
BASE COUNT         4546 a         4619 c         4505 g         4435 t
ORIGIN      
        1 atcgaagttt attaaacccc ttttgcatcc attagtgtct ttaggaatat tcgctataaa
       61 ataagggttg tgcttaatgc tttaataaaa atagcaattt tcccttgaat atagtactgg
      121 tgatggaacg atgaatctgc aagctggctt ttaacaagcc agctctaaaa gaagggaaat
      181 aagaataact atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa
      241 ctataaccca ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg
      301 atcctgataa actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg
      361 gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga
      421 aaaaattccg gcaccgacgc cagctgcaat ggcacatcct aatgcagtgg cttctttgac
      481 caccggaata ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc
      541 ttttgaacct ccgcctgcaa agactaacga tgaaggatga atattcgaga aatcagcaat
      601 ttgctgcaag ttacacgctg atacaatcgc cgcattttct tccagcgcac ggaacaatgt
      661 cgctttgtta catttatccg ggtcaatgga caagttaata aaggaaggcg cagcgtgata
      721 ccaggtttta aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg
      781 cacccgactg gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat
      841 cagtttttct tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa
      901 gcttatagat tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat
      961 ttctgggtct gtcaccggcg cggctaaatt tacaacttgc tgccagaatg tgccgccaag
     1021 aaccgcggtt tgtgccggac gcacaacgcc taacccaagg caaccaagct gcacgtcgcc
     1081 tcctccaacg accaccggag tgcccgcctt cagaccgcag agttccgccg cttgtgaact
     1141 taccacgccc agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgtaggcc
     1201 agccatatcc agcaatgcag gtttccagtc acgggtggtt agatcaagaa gtcccgtggt
     1261 gccagcgtta gagggatcca ccgccagttc gccgctgagc atataggcca gccagtcgct
     1321 gatcatggtg atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag
     1381 taatctgggg atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc
     1441 aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg cgtgccgctc tggcatccac
     1501 attggcgcag gcccagatcg gggctccttc attattatat aaaacaatgc cttcacgcat
     1561 cgaacatgcc gaaacggcag cgatatactc cggggctatg ccggcgttgt gcagcgcctg
     1621 gcgcatacac tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg
     1681 aacgtccggt actgccaggt gccgccactc cgcctgtccc actgctattt gattgccttc
     1741 caggtcgaat atcacagccc gaatacttcc ggtacctgca tccagcgcca tcaggtaata
     1801 ctttgattct gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg
     1861 tctgttcctc tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg
     1921 taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat
     1981 tgcagcggca attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat
     2041 ggtcttcagc gagcttaaag gtaagccaat cagttcgtta tgtattttaa tatccgtgac
     2101 aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcgacc
     2161 aatcattaac tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcctg
     2221 ttgactgacg gccccaatgc cgacaatcgc cacatcggct gcttgcgcgg ctaacagaac
     2281 atctttgacg caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa
     2341 cggagccgga ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata
     2401 agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact
     2461 taagcgttgc agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg
     2521 ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc
     2581 atccgcaagc ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgagt
     2641 ttcatattcc agacagcctt caaagcgaga attaatctgt acgcgaataa tgccggactg
     2701 atgccctttc tccagcaagc gcgacacttt caaacgggtc aggccgagac gatcgctgat
     2761 ctcgctctgg gtcagcccgt cgtgatagta aaaccacgcg atccgcgcga cctgttcttc
     2821 ttcacacatt ccctgttctg aaattgccga atcgttgatt gtcataattc attcttcact
     2881 ttgaacatat ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg
     2941 gcagccacag tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat
     3001 aaattgtgat ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta
     3061 aaagcagaaa tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa
     3121 cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag
     3181 gggtcaatgt cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc
     3241 tcggcggcaa tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg
     3301 ctgatagcgg tacgctggag attgggggca acaactacgc cagattaacg ccagttcatg
     3361 ctcatcagct gggcatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga
     3421 taaaagaaaa catcctgttt gggatggcaa aaaaacagct ctccatgcaa aaaatgaaga
     3481 acttgctggc ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg
     3541 tcgccgatcg ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga
     3601 tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttagtcgct
     3661 tgcaagagct gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa
     3721 ttcgccagat tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca
     3781 aaaccagcga actgtctacc gacgacatta ttcaggccat caccccagcg gtacgggaaa
     3841 aatcgctctc tgccagccaa aaattatggc tggagttacc tggtaaccgc ccacaacatg
     3901 ctgccggaac gccggtgctg acactggaaa atctgaccgg cgaaggtttc aggaatgtca
     3961 gcctgacgct caatgccgga gaaattctgg gcctggctgg gctggtggga gccggacgca
     4021 cagaactggc cgagacgctc tatggtctgc gtactttgcg tggcggacgc attatgctga
     4081 atggtaaaga gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc
     4141 tgccggaaga tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct
     4201 gcgcccttac tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc
     4261 tggaacgtta tcgtcgggcg ctgaatatta aatttaacca accggaacaa gctgcacgga
     4321 cattatccgg tggcaaccag caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc
     4381 aagtattgat tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcacgt aatgatatct
     4441 accagctgtt gcgcagcatc gccgcacaga atgtggctgt gctgcttatc tcctctgatc
     4501 tggaagagat cgaactgatg gcagatcgtg tgtatgtgat gcatcagggc gaaattaccc
     4561 actctgcact gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg
     4621 atagtcagcg tcaggaggca tcatgctgaa gtttattcag aacaaccgtg aaatcacggc
     4681 attgctggcg gtggtgctgc tgtttgtatt ccccggtttt ctcgaccgcc agtatttaag
     4741 tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg
     4801 cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat
     4861 gtgcgcggtg ctgttgggga tgttactgaa cgcaggatat tcactacctg ttgcctgtgt
     4921 cgcgacttta ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct
     4981 aaagatccct gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt
     5041 gctgtggact ggcggcaaat ggattgaagg gttacccgcc gaactgaaag agctctccgc
     5101 accgctgctg tttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat
     5161 ggcctggctg ctggcaaaga cggcgtttgg acgcagtttt tatgccacgg gcgataattt
     5221 acagggcgct cgtcaactgg gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt
     5281 gaacggctgc atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc
     5341 caaccagacc ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat
     5401 tagtttgctc ggcggttccg gtgcgatcat tggtgcggta ctcggcgcat ggttcctgac
     5461 gcagatcgat agcgtactgg tgctgttgcg cattccggca tggtggaacg attttatcgc
     5521 gggcctcgtg ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg
     5581 taatctacgg cggcaaaaat atgcccgctt tatgacgtca ccgccatccg ttaaacccgc
     5641 ttcgtcaggt aaaaatcggg aggccgcata atgcgtattc gctacggttg ggaactggct
     5701 cttgccgcac tgctcgttat tgagattgtc gcatttggtg caattaaccc gcggatgtta
     5761 gatctcaata tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta
     5821 ccgctgacga tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc
     5881 ctctgcgcca ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgata
     5941 ctcctgacct tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat
     6001 accaaagtta acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgct
     6061 ctgctgcttt ccggtatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg
     6121 gcgtttacag atttcgctaa cctggatgtg ctgggactcc ccgttccgct gattatcttc
     6181 ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg
     6241 tttttgattg ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgcacc
     6301 ttatgtgcgc tctatgccat gacggggctg gcgtctgcgg tcgccgctgt gctgctggtg
     6361 tcgtattttg gttcagcacg ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc
     6421 gccgtggtgc ttggcggtgc caatatttat ggtggttccg gttccattat cggcaccgcc
     6481 attgcggttt tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat
     6541 caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt
     6601 agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca
     6661 taaaggatat cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg
     6721 gcattgccgc aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac
     6781 tggttggcgt gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc
     6841 tgagcgttga tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt
     6901 tgattaataa cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg
     6961 atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg
     7021 actctgatac taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt
     7081 tgggaggtat gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg
     7141 cgtttttcta ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag
     7201 cgaaaatcgc caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg
     7261 atgccactaa atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg
     7321 ccattatcgc ccccgacgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga
     7381 aaaatgacaa agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg
     7441 aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag
     7501 tttatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata
     7561 tccagggcgt aggtcaggtg gaagtctcgc caaaccgcgt tcagggctat gactacgaag
     7621 cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg
     7681 gcaaatatga tttctgatgt gcattactta accggagtaa gttatggcag atttagacga
     7741 tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttaccct
     7801 gaaaggttgc ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc
     7861 gaaaacgggt aaaaccgtga tgctggcttt tgaccatggt tattttcagg gaccgactac
     7921 cggacttgaa cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat
     7981 gtgtacgcgc ggcattttgc gcagcgtagt tccaccggcg accaataagc cggtggtact
     8041 gcgggcgtca ggtgcgaact ctattctggc ggaattaagt aatgaagccg tggcgttatc
     8101 gatggatgat gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag
     8161 cgaatatgaa catcagtcga tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt
     8221 gggaatgccg accatggccg tgactggcgt gggcaaagat atggtgcgcg atcagcgtta
     8281 tttctcgctc gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta
     8341 tgtcgaaaaa ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg
     8401 cggtaaaaaa ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg
     8461 cgcttctggt gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat
     8521 gaaagccgta caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta
     8581 tctgagtgaa aaacagtaac tgcggattta aggagaagaa ttatgcacgt cacactggtt
     8641 gaaattaacg ttcatgaaga caagattgac gagtttatcg aagtttttcg ccagaaccac
     8701 ctgggctctg tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccagaagtg
     8761 aattcgcgct tttatatcta cgaagcctat aaagatgaag acgcagtggc attccataaa
     8821 accacgcccc actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt
     8881 aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat tcatcagttt tatctacaat
     8941 tggggtaacg cgctgacggg agtaaataaa tgtctgactg gaacccctct ttatatctgc
     9001 actttgccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagta ccgctggaga
     9061 atatcgaata cgttgccgat cttggctgtg gcccaggtaa cagcaccgcc cttctaaacc
     9121 aacgttggcc tgctgccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag
     9181 cgcgcagtgc tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg
     9241 aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg
     9301 aattgtttcc tcatctggtt tctttactta atccgcaggg cgtgctggca gtacagatgc
     9361 cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact
     9421 acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg
     9481 aagccggatg tgaggtcgat atctggcgaa ccacctacta tcaccagatg ccgtcgcacc
     9541 aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg
     9601 agagcgaaca gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac
     9661 tgcaagagaa cgaacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta
     9721 cggagtaatt atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct
     9781 gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt
     9841 aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa
     9901 ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc
     9961 agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag
    10021 gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca
    10081 ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga
    10141 accgccaacc attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt
    10201 acacgctgaa gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg
    10261 cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca accgggcaaa ctcccttaca
    10321 cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc
    10381 aactgccccc aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa
    10441 ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg
    10501 cccagatgcg tgtaccaggg cagcatgaaa ataacagcga tagaaaagag aaaattgagt
    10561 agtaagcgcg atgatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc
    10621 cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtgaaaaagt cgccctatta
    10681 aacgtgatta cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca
    10741 ttaacaggcg ctgcatgtgg catctgttaa tgataaaggc acataggatg ttgtaactaa
    10801 tatagtgaaa taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca
    10861 acggacgtac agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct
    10921 gctcgaccag accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca
    10981 caatcgctac cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct
    11041 gataacgttc cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac
    11101 cacgatagaa ggcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgcctt
    11161 ttgccttctg ccctgccagc agctgcggca ggatgcgagt gcggaacttg gtcataccgt
    11221 tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg
    11281 cactggcgaa agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt
    11341 agatagcttt ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat
    11401 ccagccctgc ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca
    11461 ctttgcgttc tttgtacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt
    11521 ccagacgcag ttcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt
    11581 cagcggtgtc gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat
    11641 cgcgcggata accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat
    11701 ccaaccattg aataaatgct tctggcagtg cccactcttg agcatagcgc agcaccagtt
    11761 cacgcaacgc gtcgccattg tagtcgatca actcacacgg gatgatgatc caacctttat
    11821 ccagcgcacc gttgaaatgg ctaaaacgtt cgaacagcag acgggtcagt tttgcgggat
    11881 agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg
    11941 tggtgttaga gaaaacaaag cgcatttccg gattgtgcgc cagtttcagg aattcatcgt
    12001 attcactgta gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg
    12061 cttccccctt ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca
    12121 gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg
    12181 tgtgctcatt caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac
    12241 caaactgaat gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt
    12301 tcacaatggg ttcccttctg attagtcata caacctgttt gaattggtac gacaggttag
    12361 caaactttaa tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt
    12421 atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa atacgccttt tcctggatca
    12481 taaagtggta gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact
    12541 tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga
    12601 cacgtatcgg cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg
    12661 cgaccactac cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat
    12721 ggttgcagtt caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc
    12781 accgacggca ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca
    12841 ccgacaatct tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt
    12901 ccatagttat cgttaatgct tttgaaatag tcgatatcaa gcagcatgac agtcagatgt
    12961 tgtgtctgtt tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc
    13021 tgagtcagaa aatcaaagtc ggctcgcagc gcaacttgct tcattagcga attgatcgct
    13081 gccacgctaa aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg
    13141 gagaacattt gcggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc
    13201 accagtacga tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta
    13261 tagcgcactg cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca
    13321 atgactaccg aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct
    13381 ttaaagcgcg gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg
    13441 cccgttgaga attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca
    13501 atactgaccg aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa
    13561 ttaaatagcc gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc
    13621 acgaccagta aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa
    13681 accagtcccc attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca
    13741 tagtaatgca gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc
    13801 agcggccaga aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac
    13861 agcgtggtta gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa
    13921 cggaaagttg aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca
    13981 attatctcaa cgtaataagt aaattcacat aaaggatagc atgttcgcta acagcggcgg
    14041 caaataaagg taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc
    14101 atctgtacaa ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt
    14161 aacgaaacac cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg
    14221 gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta
    14281 gccggtaatg ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact
    14341 ggcgcgcctt tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga
    14401 aattttgaac gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac
    14461 gccctaactg tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat
    14521 catccagttc cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac
    14581 cgccaccaac gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct
    14641 ggtacatacc actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca
    14701 caggttcatc aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca
    14761 gctctacaca gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca
    14821 cgtcatgatg gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg
    14881 aatgttcaaa ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta
    14941 agccgcgcac gacttccagc atacgttgcc gtggtgcgct tagtcgccct tgcaacatat
    15001 cgcagaccac cagcgcaccg gcattaatga acggattacg cggaataccc tgttccattt
    15061 ccagttgcac taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga
    15121 tttcctcttc ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact
    15181 gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga
    15241 tagcaatccc caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt
    15301 taccctgacc aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca
    15361 tggcgactgc cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag
    15421 agctgaatat gtcgcgtcag atccggtctt tccacaccgt ctggatatta cagaattcgt
    15481 gtaagccgaa atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca
    15541 ctcgcgcgtc gctggcacaa taactattga taaacacccc accgcattcc agacgtgccg
    15601 ccatctgtct ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac
    15661 tatcattagc cagttccagc gcatgttctg catctttcgc aacggtgatt gccgcaacag
    15721 ggccaaacat ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg
    15781 gcggatagta gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct
    15841 gcgccagggt tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg
    15901 gtccgagagc gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgcca
    15961 caaaacgttc ggtaaatgca gaagcaattc cctcttcgat aataaagcgt tttgccgctg
    16021 cacaaacctg tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca
    16081 gatcggcatc gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt
    16141 tcagtgccgc tccagcctgt gcgccaatag ccgctcccgc acgaacactt ccggtcaccg
    16201 tgacagcagc aatgcgcgaa tctttaatca tttgactgac accgtcgttg tcggcattca
    16261 gccagccata tacgccttgc gggattcccg catctttaaa cacctgggca atgagctgag
    16321 cacagcccat cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg
    16381 gaaccgcgcc acgcatcacc tgccataacg gaaagttcca cggcataatc gccagaatcg
    16441 tccccaacgg tcgatactca ataactgcct gctgattttc caccagcgta ggttccgcct
    16501 tcagcattgc cggaccatgt tctgcatacc agtcacacaa attcgccgat ttcgccactt
    16561 cagcgcgcgc ctgattgatt ggtttgccca tttcacgggt gatcatttgc gccatttctt
    16621 cgctacgggc gcgcagagcc ttaccgatac cacgcagttt ttcagcacga taatctatat
    16681 ttgtctcgcg ccagtcgcga aagcctgctg ccgccagctg aagtgcgttt tcgatatcgt
    16741 tagcgccagc ccacggcagc acagaaagtt gttcacccgt ggcaggattt attgaaattg
    16801 catgagttgc cggagtaatg gtcatcgggg tatctccttt atgagtcatg gtatgaagat
    16861 acgcagattt actcttgctt taaaatgaat aatattaagc cacttattca cgaatcgaga
    16921 atattatgga tctgacacaa ctggagatgt tcaacgcggt tgccgaagct ggcagcataa
    16981 cccaggcagc agcaatagtg catcgcgtgc cgtcgaatct cactacccgt ctacgccagc
    17041 tggaaacaga actgggggta gatctgttta ttcgcgagaa tcagcgttta cgtctctctc
    17101 ctgccgggca taacttttta cgctatagcc aacaaattct cgcgttagtg gatgaagcgc
    17161 ggagcgttgt cgctggcgat gagccgcaag gtttattttc tcttggttcg ctggaaagca
    17221 ccgcagcagt gcgcattccg gccacgctgg cggaattcaa ccatcgttat cccaaaattc
    17281 agttttcact ttccaccggc ccttccggca ctatgctgga aggagtgctg gagggaaaac
    17341 tgaatgcggc atttattgat gggcccatta accatactgc cattgacggg ataccggtat
    17401 accgcgagga gctgatgatc gtcacgccac aaggtcatgc gccagtaatc cgcgccagtc
    17461 aggttaacgg cagtaacatt tatgccttcc gcgccaattg ttcgtatcgt cgccacttcg
    17521 agagctggtt tcatgctgac ggtgccgctc cgggaactat tcacgagatg gagtcgtatc
    17581 acgggatgtt ggcctgtgtg gtcgcaggag caggcattgc gcttattcca cgctctatgc
    17641 tggaaagtat gccggggcat caccaggttg aagcgtggcc gttagctgag caatggcgtt
    17701 ggttaacaac ctggctggtc tggcgtcgcg gtgcgaaaac gcgtccgctt gaggcattta
    17761 ttcaactact ggatgtgtct gactcggcaa aacagagtta ccaatgagct attttgatag
    17821 ttcttgcggc taatatgctc tatatagtga tgttccgatg acttatgact atatggggca
    17881 aatatggtta cgccagtaag catcagcaat tacatatctc ttcctgatga ttttcccgtt
    17941 cgtaatattg caccacaggt aaaagaagtt ttaaaggact ttattgatgc gctaagtaca
    18001 ataatatgtg atgaagagtg gcgcacgtct ttaaacatca actcagccac aaaaaagata
    18061 tttaataacc ttgataactt atcatatatt cagaggactt ctttt
//
LOCUS       DAZFFI010000088        17955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFFI010000088 DAZFFI010000000
VERSION     DAZFFI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.088"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            302..1354
                     /gene="yhiM"
                     /locus_tag="ACHJ12_003569"
     CDS             302..1354
                     /gene="yhiM"
                     /locus_tag="ACHJ12_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4991303.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1669..2871)
                     /gene="yhiN"
                     /locus_tag="ACHJ12_003570"
     CDS             complement(1669..2871)
                     /gene="yhiN"
                     /locus_tag="ACHJ12_003570"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4991304.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3103..4602
                     /gene="pitA"
                     /locus_tag="ACHJ12_003571"
     CDS             3103..4602
                     /gene="pitA"
                     /locus_tag="ACHJ12_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4991305.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4673..5008)
                     /gene="uspB"
                     /locus_tag="ACHJ12_003572"
     CDS             complement(4673..5008)
                     /gene="uspB"
                     /locus_tag="ACHJ12_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4991306.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5399..5833
                     /gene="uspA"
                     /locus_tag="ACHJ12_003573"
     CDS             5399..5833
                     /gene="uspA"
                     /locus_tag="ACHJ12_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4991307.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5932..5985)
                     /locus_tag="ACHJ12_003574"
     CDS             complement(5932..5985)
                     /locus_tag="ACHJ12_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991308.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6151..7620
                     /gene="dtpB"
                     /locus_tag="ACHJ12_003575"
     CDS             6151..7620
                     /gene="dtpB"
                     /locus_tag="ACHJ12_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO4991309.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7669..8421)
                     /gene="rsmJ"
                     /locus_tag="ACHJ12_003576"
     CDS             complement(7669..8421)
                     /gene="rsmJ"
                     /locus_tag="ACHJ12_003576"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4991310.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8429..10471)
                     /gene="prlC"
                     /locus_tag="ACHJ12_003577"
     CDS             complement(8429..10471)
                     /gene="prlC"
                     /locus_tag="ACHJ12_003577"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4991311.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10674..11516
                     /gene="rlmJ"
                     /locus_tag="ACHJ12_003578"
     CDS             10674..11516
                     /gene="rlmJ"
                     /locus_tag="ACHJ12_003578"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4991312.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11588..12940
                     /gene="gorA"
                     /locus_tag="ACHJ12_003579"
     CDS             11588..12940
                     /gene="gorA"
                     /locus_tag="ACHJ12_003579"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4991313.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12994..13077)
                     /gene="dinQ"
                     /locus_tag="ACHJ12_003580"
     CDS             complement(12994..13077)
                     /gene="dinQ"
                     /locus_tag="ACHJ12_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4991314.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13817..14170
                     /gene="arsR"
                     /locus_tag="ACHJ12_003581"
     CDS             13817..14170
                     /gene="arsR"
                     /locus_tag="ACHJ12_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4991315.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14224..15513
                     /gene="arsB"
                     /locus_tag="ACHJ12_003582"
     CDS             14224..15513
                     /gene="arsB"
                     /locus_tag="ACHJ12_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4991316.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15526..15951
                     /gene="arsC"
                     /locus_tag="ACHJ12_003583"
     CDS             15526..15951
                     /gene="arsC"
                     /locus_tag="ACHJ12_003583"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4991317.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16085..16381)
                     /locus_tag="ACHJ12_003584"
     CDS             complement(16085..16381)
                     /locus_tag="ACHJ12_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4991318.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16488..17501
                     /locus_tag="ACHJ12_003585"
     CDS             16488..17501
                     /locus_tag="ACHJ12_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4991319.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
BASE COUNT         4199 a         4578 c         4485 g         4693 t
ORIGIN      
        1 ataatactta tatgttcgat gattttaagc accgagacta tccggtcttt tactaaataa
       61 tggaaaacaa tccatccctg gaagataatt gtaaaaaaaa caacagagta agaattaatc
      121 ttaggataaa tatttattta tcatggcctt ttaaccggta aaaaatggca ctcggcacag
      181 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
      241 caggctgaaa ttatcattaa tgcacatttc attattagca gggatacctg aggagtttaa
      301 agtgaacata tatatcgggt ggcttttcaa attaatccct ttgattatgg gattaatttg
      361 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa agcgagtatt tcgtcgcggg
      421 tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg catttattat
      481 catttcgcag ctcacgcgcg gcgttaatac gttttacaat acattgttcc ccattattgg
      541 ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag gcaatgatgt
      601 gatggcagac gagtttgtcg ccggccatgt tattttcggc gttggtatga ttgccgcctg
      661 tgtatcgacg gtggcagcgt catccggtca ctttctgctc attcccaaaa atgcagcggg
      721 gagcaagagc gatggaacgc cggtacaggc ttattcttca ttaatcggta attgcctcat
      781 tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc tgttacgtag
      841 tgctgacata actccgcatt atgtcgcggg tcacgtattg cttgggttaa ccgcaatctg
      901 tgcctgtcta attggccttg ttgccacaat tgtccatcaa acacgtaata cgttttcaac
      961 taaagaacac tggctgtggt gttattgggt tatttttctc ggctcaatca cggtactgca
     1021 ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg gcattattct
     1081 tatttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac tggcactggt
     1141 ctggagacgt acctgttcgt tagccaacag aataccgatg atacccgtct tcacctgcct
     1201 gttttgcctt ttcctggcat cgtttcttgc ggaaatggcg cagaccgaca tgggatattt
     1261 tattccttcg cgagttctgg tcggtttggg agcggtatgc tttacgttgt tctcaatcgt
     1321 ttcaatatta gaagcgggtt ctgctaaaaa ataattgcaa cgtaccggat aaaaccagcg
     1381 ttgaccattt gcgtaacgct ggtttttctt aggcatcatg aaataacgca gcattaatgc
     1441 atagtggtta agtataaaaa acagcaaagt tactgttttt ttcaacctgt tcatatttca
     1501 taaagatctg gccactgggt gagttttcaa tcacctttcc ctccacctta tattaagcat
     1561 ggagggtttc agctcacggg ctcattagaa aataatcaca agatagccac tatcaaaatt
     1621 agctcatttt taatgcgact ctaataattt tcatctttag gaaataggtc aggacgactt
     1681 tgctgcaatc aaatcctgcg cacaagccca cgcactcgac cacgcccact ggaagttata
     1741 gccccccagc cagccggtga cgtccatcac ttcgccgatg aagtacagcc caggcacttt
     1801 gcgcgcttcc atcgtccgtg aagagagttc gttggtgtcc acaccgccga gcgtcacttc
     1861 ggcagtgcga tagccttcag tgccgttggg ttgtacgcgc cagtcggtca atgtgctaat
     1921 cagtgcctgt tggtcacgca cgttgagctg ttttagcgaa acatccggga tttgcccgag
     1981 ttgctgtaag cgttcaacca accgcttcgg tagatgaacc gccagtgtgt ttttcaggct
     2041 ctgattcgga tgtgcgttac gctgctcatt caggaaggtt tcgagatcca catccggtag
     2101 cagattgata ctgacaaatt cacccggttg ccagtagctg gaaatctgca acaccgccgg
     2161 tccagacaag ccgcgatggg tgaagagtaa gttctcacgg aaaacgatgc cgttttcagc
     2221 ggtaatcacg gaaggcaccg ccacgcccgc cagcacctgt aactcttcga gcaacggttt
     2281 atgcagagtg aatggcacca gacccgcgcg ggtcggcagc acgttgaggc caaattgttc
     2341 ggcaatctta taaccaaacg gcgacgcgcc cagccccggc attgacagcc caccggtcgc
     2401 gatgaccagc ttttcgcaac cgacagtcat gccgttcagt tcaagcgtga agcctgtttc
     2461 atccttcgcc acactcagca cttcgctacg caatctgaag gttacattgc ccttctcgca
     2521 ctcatccacc agcatgtcga caatctgctg cgcggagtca tcgcagaaga gttgtcctaa
     2581 cgttttctcg tgccaggcga tgccgtgttt attgaccaga tcaatgaaat cccactgggt
     2641 aaaacgcgcg agtgcagact tacaaaaatg cggattctgg ctcagataag cgcctggttc
     2701 gacataaagg ttggtaaagt tgcagcgccc accgccagac ataaggattt tgcgccctgg
     2761 ttttttacca ttatcgatca gcagaacccg gcgtcctgcc tgacctgcca gcgcagaaca
     2821 gaacataccc gccgcaccag cgcctataat aatggcatca aacctttcca cgttgcgctc
     2881 ctcttagaaa aaacgggcgt gaattgtaaa gattcctcag tggtcgcacc agcatcaata
     2941 ttactaaaag gaagtatttg cctgaattat ataagataat tattttttga gtgaaatcca
     3001 tacaggaggc aaatcaaaaa aagtctatat ttcactttgc ccgcgccgcg aaagtcactg
     3061 ataatgcgcc gcgttcatgt cctcaaaatg gcgtaacgtc ctatgctaca tttgtttgct
     3121 ggcctggatt tgcataccgg gctgttatta ttgcttgcac tggcttttgt gctgttctac
     3181 gaagccatca atggtttcca tgacacagcc aacgccgtgg caaccgttat ctatacccgc
     3241 gcgatgcgtt ctcagctcgc cgtggttatg gcggcggtgt tcaacttttt gggtgttttg
     3301 ctgggtggtc tgagtgttgc ctatgccatt gtgcatatgc tgccgacgga tctgctgctt
     3361 aatatgggat cgtctcatgg ccttgccatg gtgttctcta tgttgctggc ggcgattatc
     3421 tggaacctgg gtacctggta ctttggttta cctgcatcca gctctcatac gctgattggc
     3481 gcgatcatcg ggattggttt aaccaatgcg ttgatgaccg ggacgtcagt ggtggatgca
     3541 ctcaatatcc cgaaagtatt aagtattttc ggttctctga tcgtttcccc tattgtcggc
     3601 ctggtgtttg ctggcggtct gattttcttg ctgcgtcgct actggagcgg caccaagaaa
     3661 cgcgcccgta tccacctgac cccagcggag cgtgaaaaga aagacggcaa gaaaaagccg
     3721 ccgttctgga cgcgtatcgc gctgatcctt tccgctatcg gcgtggcgtt ttcgcacggc
     3781 gcgaacgatg gtcagaaagg cattggtctg gttatgttgg tattgattgg cgtcgcgcca
     3841 gcaggcttcg tggtgaacat gaatgccact ggctacgaaa tcacccgtac ccgtgatgcc
     3901 atcaacaacg tcgaagctta ctttgagcag catcctgcgc tgctcaaaca ggctaccggt
     3961 gctgatcagt tagtaccggc tccggaagct ggcgcaacgc aacctgcgga gttccactgc
     4021 catccgtcga ataccattaa cgcgctcaac cgcctgaaag gtatgttgac caccgatgtg
     4081 gaaagctacg acaagctgtc gcttgatcaa cgtagccaga tgcgccgcat tatgctgtgc
     4141 gtttctgaca ctatcgacaa agtggtgaag atgcctggcg tgagtgctga cgatcagcgc
     4201 ctgttgaaga aactgaagtc tgacatgctt agcaccatcg agtatgcacc ggtgtggatc
     4261 atcatggcgg tcgcgctggc gttaggtatc ggtacgatga ttggctggcg ccgtgtggca
     4321 acgactatcg gtgagaaaat cggtaagaaa ggcatgacct acgctcaggg gatgtctgcc
     4381 cagatgacgg cggcagtgtc tatcggcctg gcgagttata ccgggatgcc ggtttccact
     4441 actcacgtac tctcctcttc tgtcgcgggg acgatggtgg tagatggtgg cggcttacag
     4501 cgtaaaaccg tgaccagcat tctgatggcc tgggtgttta cccttccggc tgcggtactg
     4561 ctttccggcg ggctgtactg gctctccttg cagttcctgt aatcgtacac accaaaacga
     4621 gcgggtcagt ttactggccc gcttttttta tgcgttatta ttgctgaagt gcttaatgcc
     4681 aaatcatcaa tgcaatcagg ctgaccacca ccagaccaca caatgcgctg gtcagaataa
     4741 actgccgacg cacccgctca cagcggcgaa taaattcatc atcgtgatga tcgcgataac
     4801 gttgggcata gatataccaa acgagacgca cctgtttgtt gggttggcca tgtgaggtaa
     4861 aaaagccccc tccatcaaca tattgataga gcaatggatc gcagttacgc agtaccacta
     4921 acaacgcgcg tagtgatgag aaatagcgcg ccatgttaac aatgcaaacg acacataaag
     4981 cccaaaataa tgcgacggtg cttatcatac ctcctccccg gcgacctgcc cgcggagttc
     5041 caccccgggg ctaccgctcc cgatacgctg ccaatcagtt aacaccaggt cctggagaaa
     5101 ccgcttttgt ggtgaccaac atccgagcgg ctctatagat agtgtaggag atcaggttgt
     5161 tttttttcca gaaggttaac cactatcaat atattcatgt cgaaaatttg tttatctaac
     5221 gggtaagcaa ggcggattga cggatcatcc gggtcgctat aaggtaagga tggtcttaac
     5281 actgaatctt tacggctggg ttagccccgc gcacgtagtt cgcaggacgc gggtgacgta
     5341 acggcacaag aaacgctagc tggccagtca tcgacaactt tatggaagga gtaacactat
     5401 ggcttataaa cacattctca tcgcagtcga tctctccccg gaaagcaaag ttctggtaga
     5461 gaaagcagtc tctatggctc gcccctacaa tgcgaaagtt tctctgatcc acgtagatgt
     5521 aaactactct gacctataca ccgggcttat tgatgtgaat ctgggtgata tgcagaaacg
     5581 catctctgaa gagacacatc atgcgctgac cgagctttcc actaatgcag gctacccaat
     5641 cactgaaacc ctgagcggca gcggcgacct gggccaggtt ctggtcgatg caatcaagaa
     5701 atacgatatg gatctggtgg tttgtggtca ccaccaggac ttctggagca aactgatgtc
     5761 ttccgcacgt cagctgatca acaccgttca cgttgatatg ctgattgttc cgctgcgcga
     5821 cgaagaagaa taatcatccc tccacgacgt gttcctgaac gcccgcatat gcgggcgttt
     5881 tgctttttgg cgcgccttgt tacctgatgc agcgtaaacc ccttatctgg cctacgatct
     5941 gcgtacgcaa tcaaaatctc ctgccaatac aacatttaac accatcatat tttccatcat
     6001 tagtgtgatc agctggttat tttctgttgt aatagtgtat taatctattc accgcatcaa
     6061 tattaagaat ctctgacaga tgtaaacttt ttcgcgcgtt atcccttacg cgttcatact
     6121 tttcaggatg gtattggaag gttaataaat atgaatacaa caacacccat ggggatgctg
     6181 cagcaacctc gcccattttt catgatcttt tttgtcgagt tatgggagcg attcggctac
     6241 tacggcgtgc agggcgtact ggcggttttc ttcgttaaac agcttggatt ctcgcaagag
     6301 caggcttttg tcacttttgg tgcttttgct gcgctggtct atggcctcat ttccattggc
     6361 ggctatgtcg gcgaccacct gctggggacc aaacgcacca ttgttctcgg agcacttgtg
     6421 ctggcgattg gctacttcat gaccggcatg tcgctactta agcctgacct gattttcatc
     6481 gccctgggga ctatcgctgt cggtaacggc ctgtttaaag ctaacccagc cagcttgctt
     6541 tcgaagtgct atccgccgaa agatccgcgg cttgatggcg cattcaccct gttctatatg
     6601 tcgatcaata tcggctcgtt gatagcgtta tcgctggccc ctgtgatcgc tgatagattc
     6661 ggttattcag tcacctacaa cctgtgcggg gcggggttaa ttatcgcatt actggtttac
     6721 atcgcctgtc gtggaatggt gaaagacatt ggttctgaac ccgacttcaa gccgatgagc
     6781 ttcagcaaac tgttgtacgt attacttggc agcgtggtga tgatcttcgt atgtgcatgg
     6841 ctgatgcaca acgtagaagt cgccaatctg gtgctgattg ttctctccat cgtcgtcacc
     6901 atcatcttct ttcgtcaggc attcaagctg gataaaaccg ggcgcaataa aatgtttgtc
     6961 gcctttgtcc tgatgctcga agcggtggtg ttttacattc tctacgccca gatgccaaca
     7021 tcgctgaact tctttgccat caacaacgtg catcatgaaa ttctcggttt ttccatcaac
     7081 ccggtcagct tccaggcgct taacccgttc tgggtggtac tcgccagccc aatactggca
     7141 ggcatttaca cgcatctggg taacaaaggc aaagacctct cgatgccgat gaaatttact
     7201 ctcggcatgt ttatgtgctc actgggcttt ttgacggcgg cagctgcggg aatgtggttt
     7261 gcggatgcac aagggctgac atcgccatgg tttatcgtgc tggtgtactt attccagagc
     7321 ttaggtgaac tgtttattag cgcccttggc ctggcgatga ttgctgccct ggtgccgcag
     7381 catttgatgg gctttattct cgggatgtgg ttcctgacgc aggctgccgc gttcttgctg
     7441 ggcggctatg tggcaacatt taccgcggtg ccggacaaca ttaccgatcc gcttgagacg
     7501 ttgcccgtct ataccaacgt gtttggtaag attggtctgg tcacgctggg cgttgcagta
     7561 gtgatgctgt tgatggtgcc gtggctgaaa cgcatgattg cgacgccgga aagccattaa
     7621 ttattcttgc agaaagcagg gtagcgttat cgctaccctg gtttactttt acactggcgt
     7681 acctgcataa atatcaaacc tatgcccttt agtgactacc gcgtttggcg tggcgacatt
     7741 cgccagcggt ggcgcgtagt ccgggcgctt taccaccacg cgtttggtcg ccagcaagcg
     7801 tgcaggctcc agtaatccat cggcatcaag atccggtccc accagcgact gaaagacacg
     7861 catctctttt ttcactagcg cgcttttctg cttatgcggg aacatcgggt cgagataaac
     7921 cacctgcggg cgcggggtaa tatcagtcag cgccgtcagg ctggaggcgt gaattaactg
     7981 caaccgctcc tgcaaccagc cgccgatttc cgcatccgca taaccacgcg ccaggccgtc
     8041 gtcgagcaat gcggcaacga ctggattacg ctccagcatc cgcacgcggc agccgactga
     8101 agccagcaca aaggcatcgc gccccagccc tgcggtggca tccaccacat ccggcaaata
     8161 atcgccttta atgcccaccg ctttcgccac cgcctcaccg cgaccaccgc cgaatttgcg
     8221 tcggtgcgcc atcgctccgc caacaaaatc aacaaagatg ccgccaagtt ttggctcatc
     8281 acgcttgcgc aattccagat gttccggcgt taacaccagc gccatcaggt tgtcttcatc
     8341 gtgctccagc ccccagcgga ccgccagaac agataaggca ccgtctccgg tgcctgtttc
     8401 atcaattaag cagattttca ctgaatgatc agcccttaat gccgtaatgc tccagcatcg
     8461 catccagctg cggttcacga ccacggaagc gtttgaacag atccatcggc tcttctgaac
     8521 cgccacggct cagaatgttg tcgaggaacg actgcccggt ttcacggttg aaaatgccct
     8581 cttcctcaaa gcgcgagaaa gcatctgccg ccagtacgtc agcccacagg tagctgtagt
     8641 aacctgcggc ataaccaccg gcgaaaatat ggctgaaagc gtgcgggaaa cggccccagg
     8701 atggagacgg tactacggca accagtttct tgatttctgc cagagtttcg aggattttcg
     8761 ctccctgatc cgggcggaac tcggcatgga ggcggaaatc gaacaggccg aactccagct
     8821 ggcgcagaat aaacagcgcc gcctggtagt tcttcgccgc cagcatttta tccagcaact
     8881 ctttcggcag cggttcgccg gtttcgtagt gaccagagat aaacgccagc gcctcaggct
     8941 cccagcacca gttttccata aactgactcg gcagttcgac cgcatcccac ggcaccccac
     9001 tgatcccgga tacaccggcg gtttcgatgc gggtcagcat atgatgcaga ccatgaccga
     9061 actcgtggaa cagggtgatc acttcgtcat gggtaaacag cgccggttta ccatttaccg
     9121 ggcggttaaa gttacaggtc agatacgcga ccggtttttg cagagaaccg tcagctttgc
     9181 gcatctggcc tacgcagtca tccatccacg ccccgccgcg cttgttttca cgggcataca
     9241 ggtcgaggta gaagctaccg cgcagttcgt tgttctcgtc atacagttcg aagaaacgta
     9301 catccgggtg ccagacatca acatctttac gctctttagc ggtgatgccg taaatacgtt
     9361 tcaccacttc aaacaaaccg ttaaccgctt tgttttccgg gaagtacgga cgcagctgtt
     9421 cgtcactgat gctgtagagg tgctgttttt gtttttcgct gtagtacgcg atatcccacg
     9481 gctgcaactc atcgacgcca aattcggctt tggcaaaggc gcgcagttgt gccagctctt
     9541 tttcgccttg cggacgcgcg cgttttgcca gatcggttaa gaaatccagc acctgctgcg
     9601 ggttttctgc cattttagtg gcaagggatt taaaggcgta gttttcaaag cccagcagtt
     9661 gcgccagttc gtgacgcaga gcgaggatct cttccatcac cttgctgtta tcccatttac
     9721 cggcgttcgg gccttgatcg gaggcgcggg tgctgtaagc gcgatacatc tcttcacgca
     9781 aggcctggtt gtcgcagtag gtcattaccg gcaggtagct tgggatatcc agcgtcagca
     9841 ggtagccttc cagctctttc gcttcggcct gggcttttgc cgcagccagc gcgctttcag
     9901 gcatccccgc cagctccgct tcgtcggtaa cgagtttggt ccagcccatc gtcgcatcga
     9961 ggacgttgtt gctgtactgg ttgcccagct cggaaagacg agtggcaatc tcaccgtagc
    10021 gttgttgttt ctctattggc agaccgatgc cagagagttc gaagtcgcgc agtgcgttat
    10081 caaccgcttt tttctgagcc gtgttcagcg tggcgtaatg atcgccatcg cgcaggtcgc
    10141 gatacgcttt atacagccct tcatgttgcc ctacccaggt gctgtattcc gacagcagcg
    10201 gcagggtttg ttcgtacgct tcacgcagtt ccgggctatt tttcaccgag ttcaggtggc
    10261 tgaccgggga gaagatacgc cccagcacat cgtccacttc cgccaacggc tggcagagat
    10321 tttcccaggt gtacggtgct ccttgcgcta ctacgcgctc cacattttcg cggcagtcgt
    10381 tcagcgcctt agtcacggct ggaacgacat gttccgggag aattttagaa aacggaggca
    10441 attcaaaggg agtcagtaac ggattcgtca ttcgcgcagt cctggttaaa gaggttaagg
    10501 aagcgctcaa caggcgcttt acataatgtg tgtagcatgg ggttaagtgt agtgaatttc
    10561 aatgagaaac gttacgcttt cgcggcggcg gcctcttttc ggtatactgt cctgatacgc
    10621 ttttgtgcgc cccgaatacg ggccgatttt tacttaccgg aacaccttta cccatgctca
    10681 gttatcgcca cagctttcac gctggcaacc acgccgacgt ccttaaacat accgttcaga
    10741 gcctgatcat cgagtcgctg aaagagaaag ataaaccgtt tctctatctc gacacccacg
    10801 caggggccgg gcgttatcag ttaggcagcg aacatgccga gcgtaccggc gaatatctcg
    10861 aaggcatcgc ccgtatctgg cagcaggacg atttgcccgc agaactggag gcgtacatca
    10921 atgtggtaaa acacttcaac cgtagcggtc agttgcgtta ctaccccggt tcgccattga
    10981 ttgctcgcca gctactgcgc gaacaagaca gcctgcaact gaccgaactg cacccgagcg
    11041 attacccgtt gctgcgttct gaatttcaga aagacagccg tgcgcgcgtc gaaaaagccg
    11101 atggtttcca gcagcttaag gccaaactgc cgccggtttc ccgccgtggt ttaatcctta
    11161 tcgacccgcc gtatgaaatg aaaaccgact atcaggcggt ggtcagcggg atagcagaag
    11221 gttacaaacg tttcgccact ggtacttacg cactgtggta tccggtggtg ctgcgtcagc
    11281 aaattaagcg catgatccac gatctggaag cgaccggtat tcgcaaaatt ctgcaaattg
    11341 aactggcggt actgccagac agcgatcgcc gtggcatgac cgcttccggc atgattgtga
    11401 ttaacccgcc gtggaaactg gaacagcaga tgaataacgt gctgccgtgg ctgcacagca
    11461 aactggttcc ggcaggcacc gggcacgcca ccgtaagctg gatcgtgccg gagtaattgc
    11521 agccattgct ggcacctatt acgtctcgcg ctacaatcgc ggtaatcaac gataaggaca
    11581 ctttgtcatg actaaacact atgattacat cgccatcggc ggcggcagcg gcggtatcgc
    11641 ctccatcaac cgcgcggcta tgtacggcca gaaatgtgcg ctgattgaag ccaaagagct
    11701 gggcggcacc tgcgtaaatg ttggctgtgt gccgaaaaaa gtgatgtggc acgcggcgca
    11761 aatccgtgaa gcgatccata tgtacggccc ggattatggt tttgatacca ctatcaataa
    11821 attcaactgg gaaacgttga tcgccagccg taccgcctat atcgaccgta ttcatacttc
    11881 ctatgaaaac gtgctcggta aaaataacgt tgatgtaatc aaaggctttg cccgcttcgt
    11941 tgatgccaaa acgctggagg taaacggcga aaccatcacg gccgatcata ttctgatcgc
    12001 cacaggcggt cgtccgagcc acccggatat tccgggcgtg gaatacggta ttgattctga
    12061 tggcttcttc gcccttcctg ctttgccaga gcgcgtggcg gttgttggcg cgggttacat
    12121 cgccgttgag ctggcgggcg tgattaacgg cctcggcgcg aaaacgcatc tgtttgtgcg
    12181 taaacatgcg ccgctgcgca gcttcgaccc gatgatttcc gaaacgctgg tcgaagtgat
    12241 gaacgccgaa ggcccgcagc tgcacaccaa cgccatcccg aaagcggtag tgaaaaatgc
    12301 cgatggtagc ctgacgctgg agctggaaga tggtcgcagt gaaacggtgg attgcctgat
    12361 ttgggcgatt ggtcgcgagc ctgccaatga caacatcaac ctggaagccg ctggcgttaa
    12421 aactaacgaa aaaggctata tcgtcgtcga taaatatcaa aacaccaata ttgaaggtat
    12481 ttacgcggtg ggcgataaca cgggtgcagt ggagctgaca ccggtggcag ttgcagcggg
    12541 tcgccgtctc tctgaacgcc tgtttaataa caagccggat gagcatctgg attacagcaa
    12601 cattccgacc gtggtcttca gccatccgcc gattggtact gttggtttaa cggaaccgca
    12661 ggcgcgcgag cagtatggcg acgatcaggt gaaagtgtat aaatcctctt tcaccgcgat
    12721 gtataccgcc gtcaccactc accgccagcc gtgccgcatg aagctggtgt gcgttggatc
    12781 ggaagagaag attgtcggta ttcacggcat tggctttggt atggacgaaa tgttgcaggg
    12841 cttcgcggtg gcgctgaaga tgggtgcaac caaaaaagac ttcgacaata ccgtcgccat
    12901 tcacccaacg gcggcagaag agttcgtgac aatgcgttaa atgttaaagg gctaagagta
    12961 gtgtgctctt agcccttaat tacgtttccg ctatcagttc agaagctgaa gcagaaagcg
    13021 gatcagttcc agcagcgcaa ttaacgcccc aagaacgatg attgctttat caatcacccg
    13081 ttttctccat gcgatggagt gagaatgcat ccgcttactc atccactgcc tgtcacggcg
    13141 catgccctat tgttagataa tggcattctc actccggcca gagcatcagt taacggcacc
    13201 acccgtactt ctgaccagga ctttgaaagc gtttatgcgc attaccagag tgaaaatgcc
    13261 tcagagctaa ctggataatc atacagtaca ttcaggttat aaaaccagca tgtccttgca
    13321 atagtttcag tatggtatta gcattgatgc gttagataat ggcattctca ctccagccag
    13381 tgccaccaac tcagggctgg aaagtaaaaa accgacgcaa agtcggtttt tttacatccg
    13441 gattcggaca aggcttaata tgacgatgac ccagtgaaag tatataaatc gtcattgcga
    13501 tatataccga agtgccccct ccgccagctg aagaaatcgc taattcttgc aatgttagcc
    13561 actggctaat agtattgagc tgttagataa gaactctctc actccagcca gagccaccaa
    13621 ctcagggctg gaaagtaaaa aaccgacgca aagtcggttt ttttacgtcc tgattcagac
    13681 ctcttttcaa atgaatagcc aactcaaaat tcacacctat taccttcctc tgcacttaca
    13741 cattcgttaa gtcatatatg tttttgactt atccgcttcg aagagagaca ctacctgcaa
    13801 caatcaggag cgcaatatgt catttctgtt acccatccaa ttgttcaaaa ttcttgctga
    13861 tgaaacccgt ctgggcatcg ttttactgct cagcgaactg ggagagttat gcgtctgcga
    13921 tctctgcact gctctcgacc agtcgcagcc caagatctcc cgccacctgg cattgctgcg
    13981 tgaaagcggg ctattgctgg accgcaagca aggtaagtgg gttcattacc gcttatcacc
    14041 gcatattcca gcatgggcgg cgaaaattat tgatgaggcc tggcgatgtg aacaggaaaa
    14101 ggttcaggcg attgtccgca acctggctcg acaaaactgt tccggggaca gtaagaacat
    14161 ttgcagttaa aaatttagct aaacacatat gaattttcag atgtgtttta tccgggaggc
    14221 attatgttac tggcaggcgc tatctttgtc ctgaccatcg tattggttat ctggcagccg
    14281 aaaggtttag gcatcggctg gagtgcaacg ctcggcgcag tactggcgtt agttacgggc
    14341 gtggtccatc cgggtgatat tccggtggtg tggaatatcg tctggaacgc gacggctgcg
    14401 tttatcgccg tcattatcat cagcctgctg ctggatgagt ccggcttttt tgaatgggcg
    14461 gcgctgcacg tctcacgctg gggtaatggt cgtggtcgct tgctgtttac ctggattgtc
    14521 ctgctcggtg ctgccgttgc cgccctgttt gccaatgatg gcgcggcgct tattttgaca
    14581 ccgattgtca tcgccatgct gctggcttta gggttcagta aaggcactac gctggcgttc
    14641 gtgatggcgg ccggattcat tgccgatacc gccagcctgc cgcttattgt ctccaacctg
    14701 gtgaatatcg tttccgctga tttctttggc ctcggctttc gcgaatacgc ctcggtgatg
    14761 gtgccggtgg atatcgccgc gattgttgcc acgctggtga tgttacatct ctattttcgc
    14821 aaagatattc cgcagaacta cgatatggcg ctgctgaaat ctcccgcaga agcgatcaaa
    14881 gatcctgcta cgttcaaagc tggctgggtt gttttactgc ttctgctggt gggatttttc
    14941 gtcctggaac cgctcggcat tccggtgagc gccattgcag ctgtgggcgc gctgatatta
    15001 tttgtcgtcg ctaaacgcgg tcatgcgatt aatacgggta aagtcctgcg cggtgccccc
    15061 tggcagattg tcatcttctc gctcggcatg tatctggtgg tttatggcct gcgcaatgcc
    15121 ggattaacgg aatatctttc tggcgtactc aacgtgctgg cggataacgg cctgtgggcc
    15181 gcgacgctcg gcaccggatt cctcaccgcc ttcctctctt ctattatgaa caatatgccg
    15241 acggtactgg ttggcgcgtt gtccattgat ggcagcacgg catctggcgt tatcaaagaa
    15301 gcgatggttt atgccaatgt gattggctgc gatttgggac cgaaaattac cccaattggt
    15361 agcctggcta cgctactctg gctgcacgta ctttcgcaga agaatatgac tatcagctgg
    15421 ggatattact tccgtacagg gattatcatg accctgcctg tgctgtttgt gacgctggct
    15481 gcgctggcgc tacgtctctc tttcactttg taatgagata ctgatatgag caacattacc
    15541 atttatcaca acccggcctg cggcacgtcg cgtaatacgc tggagatgat ccgcaacagc
    15601 ggcacagaac cgaccattat ctattatctg gaaacaccgc caacgcgcga tgagctggtc
    15661 aaactcattg ccgatatggg gatttccgta cgcgcgctgt tgcgtaaaaa cgtcgagccg
    15721 tatgaggaac tgggccttgc agaagataaa tttactgacg atcggttaat cgactttatg
    15781 cttcagcacc cgattctgat taatcgcccg attgtggtga cgccgctggg aactcgcctg
    15841 tgccgccctt cagaagtggt gctggaaatt ctgccagatg cgcaaaaagg cgcattctcc
    15901 aaggaagatg gcgagaaagt ggttgatgaa gcgggtaagc ggttgaaata aagcggcgat
    15961 atccactgcc catagttggt aaagtgcgct ttgtttatgt cggatgcgac gctaacgcgt
    16021 cttatccgac ctacaaaacc gtgcaaattc aatatatggc atgaaatatg tagagcgtag
    16081 cgcatcaggc aattttgcta tttacgcagc actcctcgcg taagaattca attatcgctt
    16141 ccagcattgc gtattgcgaa acacacatca gcgtccgact ctcacggttt tgtttgacca
    16201 gtcctaccga taccagtgct gatatatggt ggctgagtgt ggagccaggt atcccaagct
    16261 gcttttgcaa ctcaccgacc ggcaaccctt gttccccggc tttgacaaga tgtttaaata
    16321 tgaacaaacg ggtcgggtga cctagctctt tcagcgcttt agcgacttct tccaactgca
    16381 tatgacaccc ctctgtctgg ttgatatttc gataatacca gaaatatctt gatctgaatt
    16441 tcatttcgat tattctggaa atatagattt atccaatgag gttacgtatg agtagttggc
    16501 ttgcgatgct gcaagatgcc gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc
    16561 tgtttatagt gattagtgcc ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa
    16621 tccagcagat gatgggggcc agaaaaggga gaggctatct cctggctgct ctgttgggag
    16681 ccgttacccc gttctgtagt tgctcgacaa tacccatgct acgtggattg ttgtcagcga
    16741 aagccgggtt tggtccgact ctcacttttt tatttgtttc accattactt aatcccatta
    16801 tcgtcggact aatgtgggtg acctttggtt ggaaagtcac cttgttgtac gcgattatcg
    16861 ccaccggcgt ctccgttctt gccagtatta tcctggattc tctggggttt gaacgtcata
    16921 tcattgccag taaaagctca tcagcaaatt gttgtgctcc agccaaagcg tcggcaaaaa
    16981 ctttgccggg gacgacatat acgccaatag aagtgagttg ctgtagtcca acggctaaag
    17041 ccattgagaa acccgtcgtt aactgttgca ataccaaagc agtggcaagt attaatccca
    17101 taaaactagc caccaaagat gcgttgcaac agtttaaaga tgtactgcca tatcttttgt
    17161 taggggtatt aataggctct tttatttatg gctttattcc ctcagcgtgg attgccgctc
    17221 atgcaggagc agataatccc ttcgccatcc cattaagcgc cgttgtcggt attccgctgt
    17281 atatccgggc tgaggcggtt atccctctgg catctgttct gatgacaaaa ggaatgggtc
    17341 tgggagcatt aatggcatta atcatcggca gtgccggcgc aagcctgacg gaagtgatat
    17401 tacttaaatc aatgttcaga atgccgatga tagctgcatt cctgacggtt atattaggta
    17461 tggctatctt gatgggctat ttgactcaat tgctatttta aatagatgcc agcagtaaac
    17521 tataagatgc atagttagtg ccatatcctt ctaagtagaa aatcatagac aggataatcc
    17581 ttaaaaatca caaatgattt aactaatgca ttgagaatga tgacggctcg gtcaacaagt
    17641 catcaacaga caataaccac gtaatcatgc ttctctgaca ttttatcgga gaagcaatga
    17701 ctctttgtct cagtaccctc ctccttttta aaaggcagat ttcagtcgct aatacaacac
    17761 atttttaaat acactttaac ctggttcatt attacacatc taaaacatca caaaaatatt
    17821 acttcacaca ctatcggaga acaaaataac tttttaaaat gagcgctttt tgatagttgt
    17881 taacgttttc ctttacgata ctatcactgc ctttttttat acacaaatat taccgggtgg
    17941 aaatgaaagg gaatt
//
LOCUS       DAZFFI010000089        17895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFFI010000089 DAZFFI010000000
VERSION     DAZFFI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.089"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..519
                     /gene="ybeT"
                     /locus_tag="ACHJ12_003586"
     CDS             <1..519
                     /gene="ybeT"
                     /locus_tag="ACHJ12_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4991320.1"
                     /translation="IVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(529..1956)
                     /gene="djlB"
                     /locus_tag="ACHJ12_003587"
     CDS             complement(529..1956)
                     /gene="djlB"
                     /locus_tag="ACHJ12_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4991321.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1953..2660)
                     /gene="ybeR"
                     /locus_tag="ACHJ12_003588"
     CDS             complement(1953..2660)
                     /gene="ybeR"
                     /locus_tag="ACHJ12_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4991322.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2824..3801
                     /gene="ybeQ"
                     /locus_tag="ACHJ12_003589"
     CDS             2824..3801
                     /gene="ybeQ"
                     /locus_tag="ACHJ12_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO4991323.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3871..4353)
                     /gene="ybeL"
                     /locus_tag="ACHJ12_003590"
     CDS             complement(3871..4353)
                     /gene="ybeL"
                     /locus_tag="ACHJ12_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4991324.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4588..7170
                     /gene="leuS"
                     /locus_tag="ACHJ12_003591"
     CDS             4588..7170
                     /gene="leuS"
                     /locus_tag="ACHJ12_003591"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4991325.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQSNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7185..7766
                     /gene="lptE"
                     /locus_tag="ACHJ12_003592"
     CDS             7185..7766
                     /gene="lptE"
                     /locus_tag="ACHJ12_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4991326.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7766..8797
                     /gene="holA"
                     /locus_tag="ACHJ12_003593"
     CDS             7766..8797
                     /gene="holA"
                     /locus_tag="ACHJ12_003593"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4991327.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8799..9440
                     /gene="nadD"
                     /locus_tag="ACHJ12_003594"
     CDS             8799..9440
                     /gene="nadD"
                     /locus_tag="ACHJ12_003594"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4991328.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9464..10075
                     /gene="cobC"
                     /locus_tag="ACHJ12_003595"
     CDS             9464..10075
                     /gene="cobC"
                     /locus_tag="ACHJ12_003595"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4991329.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10335..10652
                     /gene="rsfS"
                     /locus_tag="ACHJ12_003596"
     CDS             10335..10652
                     /gene="rsfS"
                     /locus_tag="ACHJ12_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4991330.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10656..11123
                     /gene="rlmH"
                     /locus_tag="ACHJ12_003597"
     CDS             10656..11123
                     /gene="rlmH"
                     /locus_tag="ACHJ12_003597"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4991331.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11154..13055
                     /gene="mrdA"
                     /locus_tag="ACHJ12_003598"
     CDS             11154..13055
                     /gene="mrdA"
                     /locus_tag="ACHJ12_003598"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4991332.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13058..14170
                     /gene="mrdB"
                     /locus_tag="ACHJ12_003599"
                     /gene_synonym="rodA"
     CDS             13058..14170
                     /gene="mrdB"
                     /locus_tag="ACHJ12_003599"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4991333.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14181..15269
                     /gene="rlpA"
                     /locus_tag="ACHJ12_003600"
     CDS             14181..15269
                     /gene="rlpA"
                     /locus_tag="ACHJ12_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4991334.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15409..16620
                     /gene="dacA"
                     /locus_tag="ACHJ12_003601"
     CDS             15409..16620
                     /gene="dacA"
                     /locus_tag="ACHJ12_003601"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4991335.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16731..16994
                     /gene="ybeD"
                     /locus_tag="ACHJ12_003602"
     CDS             16731..16994
                     /gene="ybeD"
                     /locus_tag="ACHJ12_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4991336.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17095..17736
                     /gene="lipB"
                     /locus_tag="ACHJ12_003603"
     CDS             17095..17736
                     /gene="lipB"
                     /locus_tag="ACHJ12_003603"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4991337.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4528 a         4507 c         4563 g         4297 t
ORIGIN      
        1 atagttgcag caattacctg tattagccaa cccaagaaaa caacgttgcg tgataaagcc
       61 atggtgaatt atgcctttga ttatttaagc tcaccaggca gtctaccgtt aaccacggca
      121 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
      181 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
      241 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tggggtggat ctacctgaaa
      301 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
      361 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
      421 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
      481 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
      541 atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
      601 gcccagacag cccagcccgc catcgtcaac gggcattggc tgcggttgcg gctccggctc
      661 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
      721 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
      781 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
      841 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac
      901 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
      961 cgtacgggcg ctgtcgccgg ggacataaag tagctgttta ccctgatcgt taaccagttc
     1021 acgagcttca atcatcatca ccactaacgg gtaataatcg acccgatgct gacggcacca
     1081 ttgcaatatt aaagcatccg cctgggtgga gggatactcg cgccagtagt cacaaagttc
     1141 tgccagcaag ctttcgcctt ctccacaata gacgcgttgc gcgtattcat aataacgcgc
     1201 atcttcatta tcaggttcag cttcccgcca cgaccgggcg taatccagaa gttcggggat
     1261 atccaggcga cctgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
     1321 gatgatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
     1381 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg
     1441 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
     1501 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
     1561 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
     1621 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
     1681 ggcggagggc agaaaacgtt cgctatcaga ggcaagtaac gcacgaaagg cgagcagaat
     1741 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
     1801 aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
     1861 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
     1921 ttcctctatt tcgagaatct tccagcaatt tttcataggt cgctcatatc cttaagcgtt
     1981 tccgggcatg cagaatagta agccaatggt tgccaggaca aggtagggta atacgcatca
     2041 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
     2101 aagaattcgc gtaataactg cagatgatca tcttcgggag actgccaata ttgccgacca
     2161 aaggtataac cggcaaagta ttgccgccag ttacgataat aacgtagagc ccgtagatga
     2221 atgcgcgact ggatccatag actttcttct tcgctcaacc agttattaag tacgcccatt
     2281 cggcttaaaa aacccatccg gacataatcc caggctttaa ttcctccctc accacagcat
     2341 tcggcggtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttag ttcagcaaga
     2401 tagtcacgcc attcacatgg cgaataacga aaccagcggt gataaaaccc tgccaaccga
     2461 gcggcatgac cgttatcgat cattcggtga attgtgcgca gcaggtcatc acgggcatca
     2521 attccccagg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggtgatgaa
     2581 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
     2641 ttttgcgact ccatgtccat ataatttatc cttaaatatt cattaaatga atattagccg
     2701 cattgcgagg ctcgtttatt tatacttttc tgacgcgaca taatacgcat aaaataaggc
     2761 ttacgcaaaa atggaacaca taaaaatata atatgtttta tctgcaacca taaggaaatg
     2821 ataatgattt tcacgtcaac ttgctgcgat aatttatcaa tagatgaaat tatcgaacgt
     2881 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
     2941 gcaattgatt ctccggacga cgaaaaagcc ttttactggc tgaagctggc cgccgagcaa
     3001 ggtcattgtg aagcacaata ttccttaggg caaaagtata ctgaggataa aagccgtcat
     3061 aaagataatg agcatgccat cttttggctg aaaaaagctg ccctacaagg ccatactttc
     3121 gcttccaacg cccttggctg gatactggat cgtggagaag accccaatta taaagaagcg
     3181 gttgtctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
     3241 tggatgtaca gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac
     3301 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
     3361 gacggaaaag gcgttgctca aaacgagaca ctcgccgcat tctggtattt gaaaagcgca
     3421 cagcagggta atcggcatgc tcagtttcaa attgcatggg attataacgc tggcgaaggg
     3481 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggggagc
     3541 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
     3601 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
     3661 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
     3721 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
     3781 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
     3841 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     3901 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     3961 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     4021 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc
     4081 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
     4141 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
     4201 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     4261 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcaac
     4321 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     4381 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     4441 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     4501 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
     4561 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
     4621 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
     4681 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
     4741 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
     4801 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
     4861 aaaaacaata ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
     4921 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
     4981 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
     5041 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
     5101 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
     5161 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
     5221 tggccagaca ccgttaaaac catgcagagt aactggatcg gtcgttccga aggcgtggag
     5281 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
     5341 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
     5401 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
     5461 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
     5521 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
     5581 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
     5641 tacggcctga acatcaaacc agttatcctg gctgctgacg gctctgagcc agatctttct
     5701 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac
     5761 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
     5821 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
     5881 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
     5941 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
     6001 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
     6061 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
     6121 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
     6181 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
     6241 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
     6301 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
     6361 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
     6421 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
     6481 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
     6541 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
     6601 cgtgtctgga aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
     6661 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
     6721 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
     6781 ctgatgaaca aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag
     6841 gaagcgctgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
     6901 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
     6961 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
     7021 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
     7081 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca
     7141 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
     7201 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
     7261 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
     7321 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
     7381 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
     7441 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
     7501 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcgt
     7561 tctttgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
     7621 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
     7681 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
     7741 gcgtctccac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
     7801 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
     7861 ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac
     7921 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
     7981 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
     8041 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
     8101 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg cgcttgcgaa
     8161 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
     8221 tgcgcgcgca aaacagctca acttagaact ggatgacgct gcaaatcagg tgctctgcta
     8281 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
     8341 gccagacggc aaattgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
     8401 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
     8461 tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg
     8521 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
     8581 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
     8641 cttaagccag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
     8701 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
     8761 ccataaaccc ctggcggacg tatttattga cggttgatat gaaatcttta caggctctgt
     8821 ttggcggcac ctttgatccg gtgcactatg gtcatcttaa acccgtggaa acgctggcga
     8881 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
     8941 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
     9001 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
     9061 cactgaaaga gtggcggcag gaacaagggc cggacgtgcc gctggcgttt attattggtc
     9121 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
     9181 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
     9241 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
     9301 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
     9361 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtactgact tacattaacc
     9421 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
     9481 tcgtcatggc gaaacgcaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
     9541 cctgaccgca cgcggtattg agcaagcgca aaatctgcat acgctgctac atggtgtttc
     9601 ctttgatctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
     9661 cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggcga
     9721 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
     9781 gtgcaatgac tggcagcatg caatccccac gaacggtgaa ggatttcagg cattttcgca
     9841 acgtgtggaa cgctttatcg caaggcttag tgaatttcag cactatcaga atattttagt
     9901 cgtcagccat cagggtgtac tgagtctgtt aatcgcccgt ttaattggca tgcctgccga
     9961 ggctatgtgg cattttcgcg ttgaccaggg gtgttggagc accattgata tcaaccaaaa
    10021 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
    10081 ctgttttttt acgggtaagc cgcaacgacc attgacagac ccgggcaggc tgatattctc
    10141 cgcagccaga cttttttcgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    10201 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    10261 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    10321 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    10381 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    10441 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    10501 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    10561 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    10621 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    10681 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    10741 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
    10801 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    10861 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    10921 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
    10981 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcgacgctt accctccccc
    11041 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    11101 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    11161 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    11221 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    11281 aaattgttcg ctttaccgac taccagactc gctctaatga aaaccgcatt aagctggtgc
    11341 ctatcgcgcc cagccgcggc attatctacg accgtaatgg tatccctctg gccctcaacc
    11401 gcactatcta ccagatagaa atgatgccag agaaagtcga taacgtgcag caaacgctgg
    11461 acgctttgcg cagcgtggtt gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    11521 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
    11581 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    11641 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
    11701 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    11761 atgatatcgg taagctaggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    11821 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    11881 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    11941 aaacgctgct ggcgggtagc cgcgcagctg tggttgtcac cgatccgcgt acaggtgggg
    12001 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    12061 gcaaagatta ttccgccttg ttgaacgacc cgaatacacc gctggtgaac cgcgccacac
    12121 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattgagcg
    12181 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctgccaggtt
    12241 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    12301 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    12361 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    12421 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    12481 ggtatcaggg tgacaccatt ccggttggta tcggtcaggg ttactggaca gcgaccccaa
    12541 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttcctcatt
    12601 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacagccg catgaaccgc
    12661 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    12721 ctaaccgccc taacggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
    12781 cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    12841 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    12901 cgcaagtggc tgtcgccatg attctggaga acggtggtgc gggtccggcg gttggtacac
    12961 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    13021 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    13081 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    13141 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    13201 taaaataggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    13261 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    13321 ggtagatgct ttcggtgcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    13381 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    13441 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    13501 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    13561 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    13621 agtgctggtg gcggcgttca ttcctattct gtggttcttc ctgatgcatg attaccagcg
    13681 ccagcgcgta atgatgctcc tggacccgga atcagaccca ctcggcgcgg gctatcacat
    13741 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcatgg
    13801 cactcagtca caacttgaat ttctacccga acgccatacc gactttatct tcgcggtact
    13861 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    13921 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    13981 cggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    14041 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcag cgctaattgt
    14101 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    14161 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    14221 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    14281 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    14341 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    14401 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    14461 tgacggcctc tggcgaagct ttcgatccga cacagttgac ggcggcccat ccaacgcttc
    14521 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    14581 ttaatgatcg cggcccctac ggcaacgacc gcgtaatttc gctttctcgc gcagcagctg
    14641 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    14701 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    14761 tgcctgcacc tcccgattta agcggtggcg cgggggcaag ttcagtgtct ggcccgcagg
    14821 gtgacattct tccggtcagt aactcgacgc taaaaagcga agatccgacc ggcgcgccgg
    14881 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta ctggaaagca
    14941 gcgaaccgac acctgcacca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    15001 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    15061 aagtcggggc cgttagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    15121 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcgtatc cagcttggcc
    15181 cattcgccag taaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    15241 tacagtcatt tattaccacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    15301 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    15361 tagtagggca ctttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt
    15421 ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc
    15481 tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc
    15541 gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac
    15601 gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc
    15661 caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca
    15721 tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg
    15781 caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgc
    15841 gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc
    15901 tacgttaacg ccctgggcct gaaaaacacc cacttccaga cggtacatgg tctggatgct
    15961 gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac
    16021 gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag
    16081 ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga
    16141 cacactgaca aagcaggtta caaccttgtt gcttctgcga ctgaaggcca gatgcgcttg
    16201 atttctgcgg tgatgggcgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg
    16261 ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc
    16321 gcctctgaac cggtttggtt tggtgattct gatcgcgctt cgttaggggt tgataaagac
    16381 gtgtacctga ccattccacg tggtcgcatg aaagatctga aagccagcta tgtgctgaac
    16441 agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag
    16501 cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt
    16561 aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa
    16621 aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact
    16681 cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca
    16741 aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt
    16801 tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca
    16861 ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg
    16921 ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc
    16981 gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc
    17041 tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat
    17101 caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccaggct
    17161 atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg gctggtcgag
    17221 cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt
    17281 gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa
    17341 caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg
    17401 accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct
    17461 cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga
    17521 attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca
    17581 tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg
    17641 aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta
    17701 ctaaacaatc cggacttcga atatattacc gcttaattcc atacctcaat ggcccaattt
    17761 acactgggtc attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat
    17821 tttctttatc acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat
    17881 taacctattg aaata
//
LOCUS       DAZFFI010000090        17503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFFI010000090 DAZFFI010000000
VERSION     DAZFFI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.090"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(300..1328)
                     /gene="epmB"
                     /locus_tag="ACHJ12_003604"
     CDS             complement(300..1328)
                     /gene="epmB"
                     /locus_tag="ACHJ12_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4991338.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1370..1936
                     /gene="efp"
                     /locus_tag="ACHJ12_003605"
     CDS             1370..1936
                     /gene="efp"
                     /locus_tag="ACHJ12_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4991339.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1988..2113
                     /gene="ecnA"
                     /locus_tag="ACHJ12_003606"
     CDS             1988..2113
                     /gene="ecnA"
                     /locus_tag="ACHJ12_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4991340.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2224..2370
                     /gene="ecnB"
                     /locus_tag="ACHJ12_003607"
     CDS             2224..2370
                     /gene="ecnB"
                     /locus_tag="ACHJ12_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4991341.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2545..2862
                     /gene="sugE"
                     /locus_tag="ACHJ12_003608"
     CDS             2545..2862
                     /gene="sugE"
                     /locus_tag="ACHJ12_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4991342.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2859..3392)
                     /gene="blc"
                     /locus_tag="ACHJ12_003609"
     CDS             complement(2859..3392)
                     /gene="blc"
                     /locus_tag="ACHJ12_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4991343.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3481..4614)
                     /locus_tag="ACHJ12_003610"
     CDS             complement(3481..4614)
                     /locus_tag="ACHJ12_003610"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4991344.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4677..5036)
                     /gene="frdD"
                     /locus_tag="ACHJ12_003611"
     CDS             complement(4677..5036)
                     /gene="frdD"
                     /locus_tag="ACHJ12_003611"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4991345.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(5047..5442)
                     /gene="frdC"
                     /locus_tag="ACHJ12_003612"
     CDS             complement(5047..5442)
                     /gene="frdC"
                     /locus_tag="ACHJ12_003612"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4991346.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5453..6187)
                     /gene="frdB"
                     /locus_tag="ACHJ12_003613"
     CDS             complement(5453..6187)
                     /gene="frdB"
                     /locus_tag="ACHJ12_003613"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4991347.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6180..7988)
                     /gene="frdA"
                     /locus_tag="ACHJ12_003614"
     CDS             complement(6180..7988)
                     /gene="frdA"
                     /locus_tag="ACHJ12_003614"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4991348.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8313..9290
                     /gene="epmA"
                     /locus_tag="ACHJ12_003615"
     CDS             8313..9290
                     /gene="epmA"
                     /locus_tag="ACHJ12_003615"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4991349.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9509..11011
                     /gene="yjeM"
                     /locus_tag="ACHJ12_003616"
     CDS             9509..11011
                     /gene="yjeM"
                     /locus_tag="ACHJ12_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4991350.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11162..14485)
                     /gene="mscM"
                     /locus_tag="ACHJ12_003617"
     CDS             complement(11162..14485)
                     /gene="mscM"
                     /locus_tag="ACHJ12_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4991351.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14507..15475)
                     /gene="asd"
                     /locus_tag="ACHJ12_003618"
     CDS             complement(14507..15475)
                     /gene="asd"
                     /locus_tag="ACHJ12_003618"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4991352.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15572..16624)
                     /gene="rsgA"
                     /locus_tag="ACHJ12_003619"
     CDS             complement(15572..16624)
                     /gene="rsgA"
                     /locus_tag="ACHJ12_003619"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4991353.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16719..17264
                     /gene="orn"
                     /locus_tag="ACHJ12_003620"
     CDS             16719..17264
                     /gene="orn"
                     /locus_tag="ACHJ12_003620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4991354.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(17277..>17503)
                     /locus_tag="ACHJ12_003621"
     CDS             complement(17277..>17503)
                     /locus_tag="ACHJ12_003621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991355.1"
                     /translation="VLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFF
                     CVFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT         4047 a         4510 c         4594 g         4352 t
ORIGIN      
        1 acataaatag ccgtactcac tctatataaa cagaaaagaa aggttaatta ttggtgttgg
       61 ctatataaag agtaacaaca gcaatgtata tgaataatta aaggttatgc aacgggcaaa
      121 gattaaattt cccagtttaa tgacaggttg ctgtacttgc gtttgttgct cttgccggat
      181 gcggcgtaaa cgccttatcc ggcctacagt tcattgcaaa tccattcaat ttcaatgcac
      241 aatgtaggcc tgataagcgt agcgcatcag gctaatgttt aacttccctg tttaatcagt
      301 tactgctggc gtagctggag atccagcggc gttttgctgg gttcgccgcc gatttctcgc
      361 gccagtttcg gcaccagata acccgacacc agtgtcagca actcacgcat aatctgccgt
      421 gcttcgtcat cactcaccat aaaatgcgcc gcgccctgta ctttatctag cacatgcagg
      481 taatacggca tcacgccggc atcgaacaac gcattactca ggtttgccaa cgtttgtgcg
      541 ttatcgttca caccacgtaa cagaacgctc tggttaagca gggtgacacc gacacggcgc
      601 aatttagcca tcgcctgacg gaacgtttca tctacctcat tggcatgatt gatgtgattc
      661 accagcaaga tttgcagcgt agaacgggca aagcgttcaa ccagcgcctc ggtgatacgt
      721 gccgggatca caatcggcag acggctgtga atccgcagac gtttgatatg cgggatggct
      781 tccagttgtg tgagcaacca gtccagctcg tgatctttcg ccatcagcgg atcgccgccg
      841 gagaaaatca tctcgtccag ttccggatgc gcagcaacat actcaagagc agtctgccag
      901 ttacgcttgt tgccctgatt ttcggcatag gggaagtggc gacggaagca atagcggcaa
      961 tttaccgcgc agccgccttt gaccagcaaa agcgcccggt tgtggtattt atgcaacaaa
     1021 ccaggcacta cgctgtgctg ttcttccagc gggtcggtgg agaatccgga agcgacgaca
     1081 aactcatctt gcgaggtaag tacctgacgc aaaagaggat cgtccggatt gcctttctcc
     1141 atgcgatcga taaatgagcg gggcacacgc agggcaaaca gctttttggc gctgcgtccg
     1201 gccaacagtt tttcgtccgc gtctatattc aaaagacgca gaagttcatc aggatcggtc
     1261 acaacatcgg caagttgcgt taaccaatct tctctggatg gggtatttag ggttacaata
     1321 tgcgccattt tgtggcttag ctaccaatta acaaatttca gagggcctta tggcaacgta
     1381 ctatagcaac gattttcgtg ctggtcttaa aatcatgtta gacggcgaac cttacgcggt
     1441 tgaagcgagt gaattcgtaa aaccgggtaa aggccaggca tttgctcgcg ttaaactgcg
     1501 tcgtctgctg accggtactc gcgtagaaaa aaccttcaaa tctactgatt ccgctgaagg
     1561 cgctgatgtt gtcgatatga acctgactta cctgtacaac gacggtgagt tctggcactt
     1621 catgaacaac gaaactttcg agcagctgtc tgctgatgca aaagcaattg gcgacaacgc
     1681 taaatggctg ctggatcagg cagagtgtat cgtaactctg tggaatggtc agccgatctc
     1741 cgttactccg ccgaacttcg ttgaactgga aatcgttgat accgatccgg gcctgaaagg
     1801 tgataccgca ggtactggcg gcaaaccggc taccctgtct actggcgctg tggttaaagt
     1861 tccgctgttc gtacaaatcg gcgaagtcat caaagtggat acccgctctg gtgaatacgt
     1921 ctctcgcgtg aagtaatgcg gttgtggtgc ggcctgcagg ctgcaccatc acttattcag
     1981 gtcagagatg atgaaacgcc ttatcgttct tgttttgctt gccagcacgc tgctcacggg
     2041 ctgtaacacc gctcgcggtt tcggcgaaga catcaaacat ctcggcaact ccatctctcg
     2101 cgctgccagc taatttttct tctcttccga aaaatcatca gatttccatc atttttggcg
     2161 atgttgtcta ttattaattt gctataggca aacataaata acattaccta aaaggaagac
     2221 gttatggtga agaagacaat tgcagcgatc ttttctgttc tggtgctttc aacagtatta
     2281 actgcctgca acaccacgcg tggcgttggt gaagacattt ctgatggcgg taacgcgatt
     2341 tctggtgcag caacgaaagc gcagcaataa gcaataacgg tacgacagtt atgtcgtgcc
     2401 gtttgttttt ttgcgatagt cacaaaggta atagttgaaa ttcccctgcc acctggcaaa
     2461 atatccgttc aaccatcagc tttgcaggac gacctgcaaa cgcctctttt caccggggac
     2521 ggccccaatt ctccggagcc tgatatgtcc tggattatct tagttattgc tggtctgctg
     2581 gaagtggtat gggccgttgg cctgaaatat acccacggct ttagtcgttt gacgccaagt
     2641 gttattactg tgacggcgat gatagtcagt atggcgctac ttgcctgggc gatgaaatcg
     2701 ttaccggtag ggacggctta tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2761 accggcattg tgctgctcgg tgagtccgct aacccgatgc gcctggcgag tctggcgtta
     2821 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2881 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2941 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     3001 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     3061 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3121 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3181 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3241 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3301 actactgtta cgccacgcgg cggcgtagga gaactgcagg caacgaccag aaatgcagct
     3361 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3421 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3481 ttactgtaga gcgttgagaa tctgccaggc ggcggcgact ctcgctggat tgggatagtt
     3541 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3601 aaatccgccg gtcgcccccg ttttatgcac ccatgatgcg cgtactgcag gagttggggg
     3661 cgtaatcgct tttacggggc gtgctgccag tgcaatttta ttgtcactgc cgttaacgat
     3721 gatgtcagga tttaccggcc agtccagcat ttcccagccc agaccctgat acatatcgcc
     3781 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagtgttt tgtcgttgat
     3841 atcacgggga ttcatattgc ttcgtaccca gcaggccata tcttcaatgg tcgacttcac
     3901 accataagtt tcagcatcta acgcccctgg cgaaacatga actgccttgc cttcgcgata
     3961 tccccaggcg taattctttt cttctgcggg cggtacatta atccacgtat ggttgagttt
     4021 gagtggctgg aagacacgtg tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     4081 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggcgc
     4141 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacctcatc
     4201 cggcacctgc aatggcaggc cgccagcagt gtaggttgcg agatgtaata gtgtgatccc
     4261 attccactgt ttagcggtaa gttcaggcca gtattttgtt gtgggatcgc ttaacttgat
     4321 ttcccctcga gcaatagcct cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4381 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4441 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4501 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4561 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4621 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatacgttag
     4681 attgtaacga taccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4741 catttgcccg caggcacgtg aattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4801 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4861 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4921 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4981 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     5041 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     5101 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5161 cgctttcggt gccagttcaa accaggtttt ggtatgcagc agagctgccg ccagagtgat
     5221 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5281 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5341 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5401 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5461 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5521 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5581 atacgccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5641 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5701 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5761 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5821 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5881 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt cataccgtcg gtgtaatcac
     5941 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     6001 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     6061 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6121 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6181 cagccattcg ccttctcctt cttattggct gcttccgcct tatcggctgc atccgcttcg
     6241 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6301 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgaggaagtt gacgtcgtca
     6361 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6421 gcggagtgcg ccatacattc agcaacgttc agaccgtggc ccagttcaat ggtgtagagc
     6481 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6541 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6601 ccttcttcca tagccagacc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6661 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6721 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6781 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6841 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6901 tcgataccgc ccatggtgta gtgtgcggtc ggacgtaccg gaatcggttc tttaaccgga
     6961 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     7021 ttctcgccga ggtgacgcag gtcgagataa accacatcgc cacgcggcgt ggagatggtg
     7081 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7141 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7201 taacggtagc catttttgtt gaccagaata ccgccttcac cgcggcagcc ttcggtcatc
     7261 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7321 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7381 ttgtaacgat aaacgcgacc cgcaccgcca gtagccataa cgaccgcgtt agcacggatc
     7441 tgcaccagcg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7501 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7561 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7621 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7681 gggcaccccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7741 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7801 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7861 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7921 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7981 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     8041 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     8101 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8161 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8221 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8281 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8341 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8401 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8461 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8521 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gccggttgtg
     8581 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8641 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8701 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8761 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8821 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8881 cgctgctaca attgctgttt acctttggcg tagagccaaa tattggcaaa gaaaaaccga
     8941 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     9001 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     9061 atgaattgac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9121 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9181 gtatgcctga ctgttccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9241 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9301 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9361 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatccagg tatcaattct
     9421 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9481 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9541 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9601 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9661 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9721 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9781 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9841 aacattcctc tatggtagcg acatgaccca gcactggcgt attgccggac tggagcctac
     9901 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9961 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
    10021 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
    10081 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10141 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10201 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10261 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10321 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10381 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10441 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10501 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10561 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10621 ggttactgtc ttcatcctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10681 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10741 tttcccattt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgca
    10801 tatgtcggca atgattgcaa cagtggttgt cgtactggtg gtgacatttg cgaacgtctt
    10861 caccatcatt caacctgtgg ttgaagctgg agactgggac agcacattgt ggatgattgg
    10921 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10981 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    11041 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    11101 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11161 attacaaact tcccgcctga cgacctttgc ccgcagaagt taatgttctc cccgtttgtt
    11221 taccattcag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11281 catggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11341 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ttgttgcaga tccaccagga
    11401 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11461 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac accacgcgcg
    11521 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
    11581 cgattatctc tttacggtcc cagtcgctga tggtggttgc gcgggtatta attttcgtca
    11641 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11701 ggatgatcag gccagagata aagttggcga aaatttcctg taaaccaaaa ccgagaccaa
    11761 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11821 cgaccagccc gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtaacccg
    11881 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11941 gcaccagctg cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa
    12001 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    12061 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
    12121 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12181 cttcgctttc atcgacttca attgcccctt ccgggctact gtgatgatgt gcttcctctt
    12241 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12301 gcctgcggcg ttggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12361 accagatggc aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac
    12421 ccaccgccga cgccagaatg gcaaccagtg gcgcgccaat catcatgttc cacagcatat
    12481 ggttggtaat gttgtcgccg ctgccctctt tgttgagata cagtgggatc ccggcctttt
    12541 tcaggctgag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12601 gcgaaccgga gaattcacgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg
    12661 gcacaataag cccgatgctc atcaggtagt agcgcatccc acgggaaaca cgttcgcgcg
    12721 gccagccaaa atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca
    12781 ctacccacag cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat
    12841 aaggccacgc ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggc aacggtgacg
    12901 cgacgagaat cgaccagaaa agagtgcgta acgtcagcca gaagtgatcc tgagtcactt
    12961 tgccgacttt cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac
    13021 tgcaaccgac cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca
    13081 tcatcacact ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat
    13141 cctgggcgat ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca
    13201 gatagcggtg cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga
    13261 ctttcagctt ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca
    13321 actcacgctg agtgcgtagt tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct
    13381 gaccgtcggc ctgatgaatt tgccgtagca gcggctgttt attaagcaga tcctcataac
    13441 gtaaccgttg cacacgcaac tgcgccattt cggtatcaag ctgttgtggt ttcggcattt
    13501 ccggcagccg tgccacctgc gcccgcaacg cttcgccgag caggttggac gatcccagcc
    13561 attgcgactg ttcacgcagc gtattcagcg cctgccgaac ctgtaacgtc tggctggtgg
    13621 cctggcgctg ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agtgccgccg
    13681 atagttcgcg gttaattttg aattgcgcga cgatgtcttt cggcaaatcg gcgctgtttt
    13741 ctgccagcaa ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctgttca
    13801 actgattacg taaggcctgc aaatacgcat ccagttgctg gctctctttt tccgccagct
    13861 ctgagcgtaa gcgcgctaat tcctgacggt tattggcaga cagctgcgcc agttccagtt
    13921 catcaacgag cgctttcagg cgtgcggagt cagactgcaa cgcgaaattt tgtgcctgat
    13981 tgagcggggt attgccagta agcgttccca ggcggcgctc gatctcattt aactgacggc
    14041 gggcgtcggt ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct
    14101 cctgctcttg ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt
    14161 cctgattcag cgcgtcggta gacatccccg gcgacacgct gcgcggttcg tcacgcatgt
    14221 tgtttaattg tgcgcgcaga gtagcggaaa gtttcgggta attatcaatg acttcttgat
    14281 attgcttgat gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcggactgga
    14341 gcgcctctac gacttccggc tgcgcgggtt tcgccgcttt tgcctgctcc agttcttgtg
    14401 agatttgttt gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca
    14461 tcagaaaagt gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt
    14521 ttttatcgtc aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg
    14581 gggccggttc agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa
    14641 ttttcgtgac agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca
    14701 ggttgataac ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca
    14761 cagaaccgtc gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg
    14821 gcgtaatggt gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa
    14881 tctgcgccat tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa
    14941 acagattcgg cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggtacgt
    15001 agatcatctc acgcagaata ccgttgcacg gcatgtgtac gcgatggtag tcacgcgggg
    15061 agaggtaagt ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg
    15121 ccagcagggc ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga
    15181 ttttacccaa ctggctgata acgccatcgg caggcatgac caggacattc ggatcggtat
    15241 cgattgggcg tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc
    15301 tggcggtgtc cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat
    15361 cgataaccag ttttgtcagc catcctgccc gcttgcttgc gccccaaccc gccaggcgag
    15421 taagccatag tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag
    15481 cctccaggcc attgttttgt cgttcctgat ccggcctaca tgccggatcc tgaaaaaaag
    15541 gggacgattc taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac
    15601 gcgtttttac ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg
    15661 cgatttttcc ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc
    15721 aatcgcgata tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt
    15781 gttccggctc caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac
    15841 cgccgtgcgg gaagtgatac agtcgtgcgg cggtcgtggt gtgctgaccg agacccgagt
    15901 tgtcagagac atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc
    15961 tggatttgcc gacgccagac tggccggcaa aaatgctgat gcgcccggtc aacgcctctt
    16021 ccagcggttt tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccaatgt
    16081 tgcggtagat atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta
    16141 tcttattgag cacaataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt
    16201 cgataatatt gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt
    16261 tggcggcaat aggtttcacg ccgtcgtaga aatccggacg cgtcaacact gaggtgcgct
    16321 catgcaccgc ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac
    16381 gccagactac gcggtcgccg gttaccagcg aacggatggt acggcgaata ttgcagcggt
    16441 gaacgtcgcc atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac
    16501 cttcatcagg ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa
    16561 gacgacgctg gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac
    16621 tcaatcgtac agactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg
    16681 tggatcaata tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc
    16741 tgatttggat cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga
    16801 ttgccacgct ggtgaccgat gccaacctga atattctggc agaagggccg accattgcag
    16861 tacaccagtc tgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg
    16921 ccagcgggct ggtagagcga gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg
    16981 caacgctcga atttttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca
    17041 gcatcggtca ggaccgtcgt ttcctgttta aatacatgcc ggagctggaa gcctacttcc
    17101 actaccgtta tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa
    17161 ttctggatgg ttttaccaag caggggacgc atcaggcgat ggatgatatc cgtgaatcgg
    17221 tggcggagct ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac
    17281 gtaagcgccg gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg
    17341 ataatttcag cacttgagat aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga
    17401 aagatttctc gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca
    17461 aaattaagta gtacgcggga atagctcagt tggtagagca cga
//
LOCUS       DAZFFI010000091        17454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFFI010000091 DAZFFI010000000
VERSION     DAZFFI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.091"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..327
                     /locus_tag="ACHJ12_003622"
     CDS             55..327
                     /locus_tag="ACHJ12_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4991356.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(328..1200)
                     /gene="rluF"
                     /locus_tag="ACHJ12_003623"
     CDS             complement(328..1200)
                     /gene="rluF"
                     /locus_tag="ACHJ12_003623"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4991357.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1412..2101
                     /gene="pepE"
                     /locus_tag="ACHJ12_003624"
     CDS             1412..2101
                     /gene="pepE"
                     /locus_tag="ACHJ12_003624"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4991358.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2192..3823)
                     /locus_tag="ACHJ12_003625"
     CDS             complement(2192..3823)
                     /locus_tag="ACHJ12_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4991359.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4043..7726)
                     /gene="metH"
                     /locus_tag="ACHJ12_003626"
     CDS             complement(4043..7726)
                     /gene="metH"
                     /locus_tag="ACHJ12_003626"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4991360.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7926..8750
                     /gene="iclR"
                     /locus_tag="ACHJ12_003627"
     CDS             7926..8750
                     /gene="iclR"
                     /locus_tag="ACHJ12_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4991361.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9068..10641
                     /locus_tag="ACHJ12_003628"
                     /pseudo
     CDS             9068..10641
                     /locus_tag="ACHJ12_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(10610..12346)
                     /gene="aceK"
                     /locus_tag="ACHJ12_003629"
     CDS             complement(10610..12346)
                     /gene="aceK"
                     /locus_tag="ACHJ12_003629"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4991362.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(12627..13931)
                     /gene="aceA"
                     /locus_tag="ACHJ12_003630"
     CDS             complement(12627..13931)
                     /gene="aceA"
                     /locus_tag="ACHJ12_003630"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4991363.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(13961..15562)
                     /gene="aceB"
                     /locus_tag="ACHJ12_003631"
     CDS             complement(13961..15562)
                     /gene="aceB"
                     /locus_tag="ACHJ12_003631"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4991364.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(15831..16760)
                     /gene="metA"
                     /locus_tag="ACHJ12_003632"
     CDS             complement(15831..16760)
                     /gene="metA"
                     /locus_tag="ACHJ12_003632"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4991365.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            16917..17360
                     /locus_tag="ACHJ12_003633"
     CDS             16917..17360
                     /locus_tag="ACHJ12_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4991366.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         4268 a         4566 c         4378 g         4242 t
ORIGIN      
        1 gctcgcaaac gaattcccgt acactgaact ctctggctca ctgagggcac catcatggca
       61 ctcccccgta ttacccaaaa agagatgact gaacgcgagc agcgtgaact taaaacactg
      121 ctggatcggg cgcggattgc gcatggtcgc gtactgacca actccgaaac caacagcatt
      181 aagaaagagt acatcgataa attaatggtc gagcgtgagg ctgaagcgaa aaaagcccgc
      241 cagttgaaga aaaagcaggc ttataaaccg gatcctgaag cgtcatttag ctggtcagca
      301 aatacgtcga cgcgcggcag gcgctgatta gcgccccttc tttttacgcc ccggagaggt
      361 aaaacgctta ccgttggacg ccgggcgacc gcctttttcc gccgtttttt ccatcttaac
      421 gactggacgt ttgatgcccg ctgttttcgg tttggccttc actttaggtt ttgcctcgga
      481 agaggaattt tcgatgagct taaagaggtc gattaactca tcatcagtta aatcgcgcca
      541 ctcacccagc ggaatgccgc ttaagctgac gttcataatg cgcgtacgtt ccagcttttt
      601 cacttcatag ccgaaatgct cgcacatgcg ccggatctga cggttcagcc cctgcaccag
      661 ggtaatgcgg aagacaaacg gcgcttcttt tttgactttg cactttttcg tcaccgtccc
      721 gaggatcggc acccccgccc ccataccacg aataaattca tcggtaatcg gtttatcgac
      781 cgtcaccaga tactctttct catgatcgtt acccgcacgg aggatcttat tcaccagatc
      841 gccgtgattg gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc caatcgggaa
      901 cacgcgtttg ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat cttcggtggt
      961 gctgacaata ccgacgggct tgttcagggc gataagtacc aaatcttcgg cttcccgagg
     1021 ctcaatcaac tgaccgttta ctttcacaac gtcgccgggt ttcacctgat cgccaatggt
     1081 ggctcgcttg ccattaagga acacattgcc ttgctcgata tagcgatccg cttcgcggcg
     1141 tgagcaaatt ccgctttcgc tgatgtattt atttaaacgg actgatgagt cgggcagcat
     1201 aaattctcct gtaaaaaacg gaatataccg tacctttggg ttgataaaaa atagtcatga
     1261 gcgaactatt tgttatttcc attggcttga attcgttacc tgctcaaatc actttccaga
     1321 agcaatgtgc gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     1381 ctcccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     1441 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     1501 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     1561 agcgaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     1621 tgtcgatccc gttgccgcga ttgaaaatgc tgagatcgtg attgtcggcg gcgggaatac
     1681 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gcaccaatta ctgacgtggt
     1741 taaacgtggc gctctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     1801 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     1861 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     1921 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     1981 accggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     2041 cacttatgtg tttaaggctg gtgaagaagc ggtgccgcta gaagctggtc accgttttta
     2101 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     2161 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     2221 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     2281 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     2341 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     2401 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     2461 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     2521 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     2581 aagcctgttc aaggttgagc gacatttcaa taatctccgc ccagcgacgc gactcttctt
     2641 ctgccagttc ctcttttggc atccgcgcca gatacagctt aatggcggta tagagaacgt
     2701 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     2761 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     2821 cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac atccagatgt ttaggccgta
     2881 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     2941 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     3001 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     3061 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     3121 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagcac
     3181 caatcaccag acagagcgcc acggggaagg agataatgcc tgcggcggtc agagtcgcag
     3241 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     3301 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     3361 tgatcggcgt tacggcctgc acaatcaact ccagcgccag taaaatcagc ccaagaccaa
     3421 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     3481 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     3541 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     3601 cctgagcgac aaacgaggtc accagcatgg tggtggcatt actgctctgc accagtgcgg
     3661 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     3721 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     3781 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca ggttctcctt
     3841 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
     3901 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
     3961 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
     4021 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
     4081 gctcaacatc ggagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct
     4141 gaatttgcgc caccgcgtag tacttgctgt ccgggtggct gaaataccaa cccgaaaccg
     4201 atgcaccggg ccacatggcg aaagattctg tgagtttcat gccggtatgt ttttccactt
     4261 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccggg tagcccggtg
     4321 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
     4381 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
     4441 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
     4501 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
     4561 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
     4621 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
     4681 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
     4741 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
     4801 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
     4861 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagagtt tcgatgctgg
     4921 cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
     4981 aagcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgc ggtttcttac
     5041 gcccgtgctg aatacgtacg gtttcgtact ccttgcgggt acgagcgaca aaatcatcac
     5101 gctgagtatc ggaaagcagc gcagccacca caccaacggt gcgcgatgcg ttctgtacat
     5161 acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc cgtgtgcgct tttgaggtcg
     5221 tcgcgccgcc aatcaatagc gggatggtga agccctgacg ctccatctct ttcgccacgt
     5281 taaccatctc gtccagcgac ggcgtgataa gccccgaaag tccaatcaga tcagcattca
     5341 cttctttagc ggtacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
     5401 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacgtcgc
     5461 ccttcacggt ggcgatcacc atcttgccgt tggttttgcc ctgctctttg ctggcttcaa
     5521 taaacggttc gagatacgcc accgcctgtt tcatgacgcg cgccgatttg accacctgtg
     5581 gcaggaacat tttcccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
     5641 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttcg gtatcctgct
     5701 cgataaactc ggtaatgcct ttgaccagcg agtattccag acgtttattc acttcccacg
     5761 agcgccactc cgcctgctgg gcgttagcgg tgtcgtcggt tttgctgccg cgatatttct
     5821 cggcaagctc cagtaaacgc tcagtgccat catcgcgacg attaagaatc acatcttcca
     5881 ccgcgtcgcg cagttcagcg ggcaggtcgt cgtaaatcgc cagctgcccg gcattgacga
     5941 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
     6001 gcaccggatc gttgccacgg aacgagaaag aaacgttaga tacgccgccg gaaattagcg
     6061 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
     6121 tgtgctcttc aatgccggtg gcaaccgcga agatgttcgg gtcgaagatg atatcttctg
     6181 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
     6241 gtgcgcgggt atcggcctgt ccctgttcgt caaaggccat taccaccact gccgcaccgt
     6301 aacgacgcaa gagcttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
     6361 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
     6421 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
     6481 aacgcaccat cgccgcttcg gcgtcgagca tcccttcatc catgttgata tcgataatct
     6541 gcgcaccgtt ttccacctgc tggcgggcca catccagtgc ctcgctgtat ttctcttctt
     6601 tgatcaggcg cttgaactta gcggaaccgg tgacgttggt gcgttcaccc acgttcacaa
     6661 acaggctatc ttcgccaatg ttcagcggct ccaggccgga caaacggcag gctacgggga
     6721 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
     6781 gtggcgtggt gccacagcag ccgccgacga tattgagaaa acccgcttgc gcccattcac
     6841 gtatctgttt tgccatcgtg tcggcgtcga gatcgtactc accaaaggcg ttgggtagcc
     6901 cggcgttcgg gtgcgcggtg acgtagcatt ccgcaatccg tgacagctcc tgcacgtact
     6961 ggcgcagttc atctggcccc agcgcacagt tcaggccaaa ggtcagagct tcggcgtggc
     7021 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
     7081 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
     7141 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
     7201 ccgcaccacc ttccaccagc gctttggtgg actctcgata agccgccacc agcccgtcaa
     7261 aagtgatatt acgaaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
     7321 ttgggccgag aacaccagca acgtagcgcg gtttctccgg ggtgcgcgcg gtccactcat
     7381 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
     7441 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
     7501 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
     7561 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
     7621 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc agcaccagaa
     7681 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
     7741 cgctcaaccc agatttaata aaaattcaac aaaccatact ggcataaacg catctgtggt
     7801 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
     7861 aactattgca ttagctaaca ataaaaatga aaatgatttc cacgatacag aaaaaggaga
     7921 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
     7981 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
     8041 aatccaatgg cagtgtggca ctcacggaac tggcgcaaca agccgggtta cccaattcca
     8101 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gttggcgaac
     8161 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
     8221 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
     8281 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
     8341 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
     8401 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
     8461 accgcaaagg gttacatgcc tatacccacg caacgctggt gtctcctgtg catttaaaag
     8521 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
     8581 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
     8641 caatttccgg accgatttca cgtattaccg atgaccgcgt gaccgagttt ggcgcaatgg
     8701 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga cttttttctg
     8761 gcgggcagag gcaatattct gcccgtcata tctgagtggc aatagaataa gggtgtctgt
     8821 taatcgcatt gacgccaaaa taacttattg tcatacactt cgcacacttt tattaactca
     8881 gcattatttt taaacatcaa accacttaat tattaacaaa atcgcaagat ctcattacgc
     8941 cgaaactttt acggctgcat tatgagattg agcgatttat gatatttctt tagttgtaat
     9001 catattaaat tttttcactg ttctgataat taaaattcaa gacatcaata aacaatgaga
     9061 tatttaaatg attactcgta tccctcgtag ttctttctct gcaaatatta ataatacagc
     9121 ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg aggataaatt
     9181 ttctggcaag gagctggcga cgcctctatt aaaaagcttc tcagagaact gtagacaaaa
     9241 tggtcgtcat atctttagca acaaggattt tgtcattaaa ttttccacgt ccgtcttaca
     9301 agctgataag aaagaaatta cgataattaa taaaaacgaa aacacgacac ttactcaaac
     9361 cattgcccca atatttgaag aatacctaat ggaaatttta cctcaacgct cagacactct
     9421 tgataaacaa gaattaaacc taaaatcaga tagaaaagaa aaagaattcc caagaattaa
     9481 acttaatggt caatgttatt ttccggggcg accccaaaac cgtatagtat gccgacacat
     9541 tgctgcacaa tatattaatg atatttatca gaatgttgat tacaaacccc atcaagatga
     9601 ttactcttca gctgaaaaat ttctcacgca cttcaacaaa aaatgcaaaa accagacttt
     9661 ggcgttggtt tccagccgtc ctgaggggcg ttacgttgct gcctgtggtg atttcgggct
     9721 agttatgaag gcatatttcg acaagatgga atcaaatggc atcagtgtta tggcagccat
     9781 attattggtg gataaccatg ctttgacggc gcggctaaga ataaagaaca caactgaagg
     9841 atgtacccat tacgtggttt cggtttatga tcctaatgtc actaacgata aaataagaat
     9901 tatgagcgaa agcaaagagg atattaaaca ctattctctg atggatttta tgaatgtaga
     9961 ttatagcctc ctgaaatggt caaatgatca tattattaac caatctgttg caataattcc
    10021 agcacttccg aaagaacagc tattgatgtt aaaaggatct gtggatgaaa taacccctcc
    10081 attatcacct gcaacgatga atttgctaat ggcaattggt cagaatcacc aacttacgca
    10141 actgatgatt caactccaga aaatgccaga actacataga acagaaatgt tgactgccta
    10201 taatagtgga catatgaacg ttattaatac tatttttaac gcattaccca ctctttttaa
    10261 tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa
    10321 tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga gacggtttat
    10381 cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt
    10441 tggcaacaca aagccccaca aaaatactct gcctttgagt tggcttttga attgggtcac
    10501 cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt
    10561 acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca
    10621 tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat
    10681 gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt
    10741 gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt
    10801 ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg
    10861 gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt
    10921 aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc
    10981 caggaaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt
    11041 cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct
    11101 cgatatagag atggcgaatt acaatctgct caccaagatc ggtgattttt tccgctgctt
    11161 cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt
    11221 caaactcctg ggtgtccgcc attcggccca cacggtcgtg ctctttcacc agttgatagc
    11281 aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt
    11341 tgaatacccg atcaaagccc ggcagcgtaa ataccaacat caccattcca cgaatacccg
    11401 gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt
    11461 cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg
    11521 gcagaatttc ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat
    11581 aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa
    11641 taaataactc gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag
    11701 gcgtgatcag tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca
    11761 cctgtaaatg actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga
    11821 tgtaatgaat gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa
    11881 cgcgcatcag tagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg
    11941 gaatggtacg aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa
    12001 gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga
    12061 agcgcgggta atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg
    12121 cgtccgtact ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat
    12181 gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt
    12241 cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc
    12301 cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc
    12361 aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg
    12421 ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg
    12481 cggcgtaaat gccttatccg gcctacgttc ggcaacggct gtaggcatga taagacgcgc
    12541 aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtgaacgc cttatccggc
    12601 ctacagtcag caacggttgt tgttgcttag aactgcgatt cttcagtgga gccggtcagc
    12661 gcggtgactg aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta
    12721 cccacttcct gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc
    12781 tgctgcactt tctcaacgta gtgcttcata ccctcgccct gggcataggc gtttgccagg
    12841 tcaaacatgt tgaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag
    12901 cccatatccg acagctgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc
    12961 cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata
    13021 gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg
    13081 tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta
    13141 cggaagaagc cttcactggt acgctcgcca gtaataaatt cactgtcata cgggtcgcaa
    13201 tcggaggtaa tcagatccgc cgcatcggca tcggtacgag caaccagcag ggttggaacg
    13261 cccgtcacgt cagctgcaag acgcgccgcg accagtttct gaatagcttc ctgagttggc
    13321 accaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag
    13381 tgaactgccg ctgcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca
    13441 ccgccaaaac cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga
    13501 tcgcccggct caatgcctgc ggaccattgg atctgatcgg cacgacggaa ggtgttgttg
    13561 atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg
    13621 ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata
    13681 cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag
    13741 ccttttttcg actcgccatg cagcagacgc cacattttcg ccgcgcccag ttgcgccagg
    13801 gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcgct gtatgggcga
    13861 gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta
    13921 cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc
    13981 ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg
    14041 cggcatcgtc aaaacgcccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt
    14101 tcatctcttc acccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca
    14161 acgttttttg atgatggatc cactgccaga ttgaggtacg ggaaatttca gccgtcgccg
    14221 catcttccat caggccataa atcggcacgc agccgttgcc ggagatccat gcttcgatgt
    14281 actgcactgc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag
    14341 gtgccagcag ctgatcggca gtaatcggca cgtcttgttc gcgcatcact tcaagctgat
    14401 ttttacggga gccgagaatg tcgttgaata ccgccattgc cgtatccgca aggcctgggt
    14461 gagcaatcca tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt
    14521 tgttgagcac ctggttattg cgctcttcat ctttgctcgg aataaacgct gccatgccgc
    14581 ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcaa caggcgtgag taagcattca
    14641 ggaatggttt atccatcgtc actgcctgtc tgtctggcag gacgcgatcg ggatagtttt
    14701 tcaacgtttt gatatagctg aagatgtaat cccaacgacc gcagttcaga ccaacaatat
    14761 ggtcacgcag cgcgtgaagg atttcatcca tctggaacac ggcgggcagc gtttcaatca
    14821 gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga
    14881 cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac
    14941 cgctgccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca
    15001 ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc
    15061 gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat
    15121 tggtatagct gatggtgccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt
    15181 tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt
    15241 tcagtgcgtt gatcaccatc ttgcgctcaa ccgggccagt tatctcgact cgacgatctt
    15301 gtaagtccgc gggaatacca cgaattttcc agtccgtatc gcgaatggaa gctgtttccg
    15361 aaataaaatc aggcaaagtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca
    15421 gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt
    15481 cggcagtaag aatttgcttc tcctgctcgc catacggcct tatgaaagcc agttcatcgg
    15541 ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttccccac
    15601 tgtgaacgaa ggatcgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa
    15661 tgcaaaatca aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa
    15721 tatgaataaa atttattcac ctcctgaggt gcgtttcgga aataaaaaag gcacccgaag
    15781 gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc
    15841 gttggattca tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag
    15901 ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtgtatt ttgcggatca
    15961 ttgtgcggga aatagttata cggtacttcc gggcctagtc cggcttccac atcgcggaaa
    16021 tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc
    16081 ttatctttgc tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga
    16141 tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg tggtgccagg
    16201 aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa
    16261 acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg
    16321 gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc
    16381 acctgtttga tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca
    16441 ccggttacaa tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag
    16501 ttgttcagat gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga
    16561 atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc
    16621 ttcttcggca tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca
    16681 cgagaagttg tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg
    16741 tccggcacac gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca
    16801 tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga
    16861 gaaagtttca aggaaaagac tacgttagaa tataagaatg actaaaagga gagaaaatgg
    16921 ttattagtat tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc
    16981 gatctgtcga tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc
    17041 tggttcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggatcagc
    17101 cggttgggtt tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg
    17161 tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac
    17221 tgacaaccaa cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt
    17281 ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc
    17341 tggcgtatgt gggggaataa gccgcccctg ccgtgcctgc ccaggctgga gaagacgcgc
    17401 cagcgtcgca cccggcattt ttttcaccaa cgcaaaaagg ccatcgtgac tgat
//
LOCUS       DAZFFI010000092        17243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFFI010000092 DAZFFI010000000
VERSION     DAZFFI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.092"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1031)
                     /locus_tag="ACHJ12_003634"
     CDS             complement(<1..1031)
                     /locus_tag="ACHJ12_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4991367.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPS"
     gene            complement(1089..1238)
                     /gene="ylcJ"
                     /locus_tag="ACHJ12_003635"
     CDS             complement(1089..1238)
                     /gene="ylcJ"
                     /locus_tag="ACHJ12_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFO4991368.1"
                     /translation="MSLVLCFLLMSLFFMYFFVLSRLWRKKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            complement(1286..1618)
                     /gene="emrE"
                     /locus_tag="ACHJ12_003636"
     CDS             complement(1286..1618)
                     /gene="emrE"
                     /locus_tag="ACHJ12_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO4991369.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1686..1988)
                     /locus_tag="ACHJ12_003637"
     CDS             complement(1686..1988)
                     /locus_tag="ACHJ12_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4991370.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(1985..2686)
                     /locus_tag="ACHJ12_003638"
     CDS             complement(1985..2686)
                     /locus_tag="ACHJ12_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4991371.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2683..3702)
                     /locus_tag="ACHJ12_003639"
     CDS             complement(2683..3702)
                     /locus_tag="ACHJ12_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4991372.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVEL"
     gene            complement(3699..4238)
                     /locus_tag="ACHJ12_003640"
     CDS             complement(3699..4238)
                     /locus_tag="ACHJ12_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO4991373.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4308..4538)
                     /locus_tag="ACHJ12_003641"
     CDS             complement(4308..4538)
                     /locus_tag="ACHJ12_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4991374.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4643..5332
                     /locus_tag="ACHJ12_003642"
     CDS             4643..5332
                     /locus_tag="ACHJ12_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4991375.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            5928..6134
                     /locus_tag="ACHJ12_003643"
     CDS             5928..6134
                     /locus_tag="ACHJ12_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO4991376.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            6210..6506
                     /locus_tag="ACHJ12_003644"
     CDS             6210..6506
                     /locus_tag="ACHJ12_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4991377.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6512..7297
                     /gene="bet"
                     /locus_tag="ACHJ12_003645"
     CDS             6512..7297
                     /gene="bet"
                     /locus_tag="ACHJ12_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4991378.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            7294..7974
                     /locus_tag="ACHJ12_003646"
     CDS             7294..7974
                     /locus_tag="ACHJ12_003646"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4991379.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDTRVLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            7971..8153
                     /locus_tag="ACHJ12_003647"
     CDS             7971..8153
                     /locus_tag="ACHJ12_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4991380.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            8126..8317
                     /locus_tag="ACHJ12_003648"
     CDS             8126..8317
                     /locus_tag="ACHJ12_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4991381.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            8394..8609
                     /locus_tag="ACHJ12_003649"
     CDS             8394..8609
                     /locus_tag="ACHJ12_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4991382.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            8708..8926
                     /locus_tag="ACHJ12_003650"
     CDS             8708..8926
                     /locus_tag="ACHJ12_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4991383.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            8974..9252
                     /locus_tag="ACHJ12_003651"
     CDS             8974..9252
                     /locus_tag="ACHJ12_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4991384.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9224..9289
                     /locus_tag="ACHJ12_003652"
                     /pseudo
     CDS             9224..9289
                     /locus_tag="ACHJ12_003652"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9451..10614
                     /gene="intD"
                     /locus_tag="ACHJ12_003653"
     CDS             9451..10614
                     /gene="intD"
                     /locus_tag="ACHJ12_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO4991385.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(10630..10706)
                     /locus_tag="ACHJ12_003654"
     tRNA            complement(10630..10706)
                     /locus_tag="ACHJ12_003654"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10670..10672),aa:Arg,seq:tct)
     gene            10949..11581
                     /gene="fimZ"
                     /locus_tag="ACHJ12_003655"
     CDS             10949..11581
                     /gene="fimZ"
                     /locus_tag="ACHJ12_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4991386.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(11584..12099)
                     /gene="sfmF"
                     /locus_tag="ACHJ12_003656"
     CDS             complement(11584..12099)
                     /gene="sfmF"
                     /locus_tag="ACHJ12_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4991387.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(12110..13117)
                     /gene="fimH"
                     /locus_tag="ACHJ12_003657"
     CDS             complement(12110..13117)
                     /gene="fimH"
                     /locus_tag="ACHJ12_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4991388.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13130..15738)
                     /gene="sfmD"
                     /locus_tag="ACHJ12_003658"
                     /pseudo
     CDS             complement(13130..15738)
                     /gene="sfmD"
                     /locus_tag="ACHJ12_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(15769..16461)
                     /gene="fimC"
                     /locus_tag="ACHJ12_003659"
     CDS             complement(15769..16461)
                     /gene="fimC"
                     /locus_tag="ACHJ12_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4991389.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(16681..17223)
                     /gene="fimA"
                     /locus_tag="ACHJ12_003660"
     CDS             complement(16681..17223)
                     /gene="fimA"
                     /locus_tag="ACHJ12_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4991390.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         4796 a         4090 c         3853 g         4504 t
ORIGIN      
        1 attgatggcc tgtcacatcg atgtaatctt ctcatcggac aaacataata gccatgcaaa
       61 cttccagata ccgcatgaac aatcatggta ttaccacaag cctcacactt cataactgtt
      121 cgaagtagat ttattagcat aggattcttg ctactattgc taataccaaa aggtgccaac
      181 cgaatttcct gtacagcgta aaacaaatca tctgaaatga ctctgggata atagccagcg
      241 atttcactta tccctttccc tcttgcacga tatgaaggta cgcatatacc tatcagagct
      301 ttattcgcta ataatttttc aattacagaa ggtccccatg cactttcttt tcctgagaaa
      361 ttctttacag catgatcatt taaatacttg gctattgcat tcaatgagcg cctttccatc
      421 ctgagtttaa aaattagctc aatagttttc accctgtcgg ggtctggaac aaaagccgtt
      481 cttttgtcat ctaaggagag ccatctcgga caagacgccg tcataatcgt acctgattcc
      541 agtgcatcct gccgtttttt cttccatgat aatttaaccc gacttgactt tatctcgctt
      601 tcttcatttg ccctttgtgc tataagtatg gcttttatta atgaatatgg ctcattcaaa
      661 gagtcaatat tatagactgt attgtcgcaa agagttataa catcaatacc gtgattcaaa
      721 atcaatttca gacgttcaat cgcttcaccg actttttctc ttgaaagtct gtccagactt
      781 tcaactaaca atgtagttcc tggcaatata taaccatgct ctatagcatc taaaaattcc
      841 gaaaaagctc ctgattgtgc atgctttcct ttgaatgcac ttaatcctaa atcttcatat
      901 gttatggtat caagataata atcactattt acctttaacc attcagcaat aagtcttctc
      961 tgtcggttta atgagtcacc agacatctga cctggtgatg aaaatcgcat atatgctatg
     1021 gcttttttca tggtgacacc tgctaacgta tgcttttata aaccttagtg gtgggatata
     1081 atttttgttt aatttttatt taaaaagaca attaaggtca cattatcttg aatatacaac
     1141 aataatcgta ttgcaatttt cttacgccat aatcttgaaa gcacaaaaaa atacataaaa
     1201 aataaagaca ttaacaaaaa gcataaaacg aggctcatgt aaatataaga gcctccatat
     1261 tttagtcgtt tagaaacaaa ttattttaat gtggtgtgct tcgtgacaat aaattaataa
     1321 ccaacacacc ggcacaaatc aacatcatgc ctataatggc tggcaggtcc agccgttggc
     1381 cgaaaaatcc ccatgacagt aagctaatca ggacaatacc gactcctgac cagatagcat
     1441 aagcaatccc tgtaggaata taagccagcg tctgagctaa taaccagaat gatgcacaat
     1501 aacaaataat tgtaccaaca gatggccata accgtgtaaa accttctgaa aacttcatta
     1561 aggttgtacc aatgacctct gcaagtattg caccaccaag ataaatataa gggttcatag
     1621 catattcttt cctgttcaaa ctggagagaa ttgtactaca gtttgaactc aactcacctg
     1681 tttcatcatt gtgttcccat tgatgttctt ttatataccc tcaatacccg tttcatcgcg
     1741 gcactctggc gacactcctt aaaaatcaga ttcgtgctca cctttccttc ccgttcttct
     1801 ctggtagcga accggtaata caccgttcgc cagaccttac catcaacgac caggattcct
     1861 gcccgcgcca ttttagccgc agcctgattt atgctggtta cggttgcgcc tgttaccgcg
     1921 gcaacgtcct gtgcacagaa gctcttatgc gtccccaggt aatgaataat tgcctctttg
     1981 cccgtcatac acttgctcct ttcagtccga acttagcttt aatttctgcg atcttcgcca
     2041 gagcctgtgc acgatttaga ggtctaccgc ccataacagg aagttgtttt actggttcag
     2101 gtatcgtctc accacggtta attcgcgctg tcatacaggt cagttcatcg gcagccttgc
     2161 gccgtaattc cgcgtcagtc agcgcattgg cccgcatgtt ctggtacaag ttggtaacca
     2221 accagtaatg cgcgttcgat ttccacggat aagactctgc atccggatac aggccacgct
     2281 tccggcaata ctcgtaaacc atatcaacca gctcgctgac gtttggcagc ccggcgttaa
     2341 cagatgcttc ttcccggcac caggcgacaa actgcccggg tgatggcagg aatggtcgat
     2401 tctgccgacg ggctacgcgc attccagcgt taacctgttc cattgtggtg atcccgtttt
     2461 cccggaaagc cagaacccac tggcggcgga tttcgttcag ttcattctgg tcacggttag
     2521 ccaggctcgc cgggaaagtt gccagtaact ggctgaacac accgttgatg atctgcgcta
     2581 cctgctgtac ctgtggcttt tcgtcgtact gttccggcat gttgttggcg atccggcgca
     2641 tctgctcaca gtcaaagtta accatctgtg cggcgatgtt tttcatagct ccaccccgta
     2701 aatccagtca gtgttcgtca ggtcgagttt tggtttgccg gctgtcacgc cagcctgttg
     2761 cttgtttcgg ttgatttcga gctgggtcca cttgtcgcgg agtttggccg gacttagcac
     2821 gttaccggac cagaagttgt cctggcatgc ccagcggaac agcacgcaca tgtcgcggtg
     2881 gttacgtccg tcacgttcac gcatcaggcg gatatcgtta gcccaccctg caaaattcgg
     2941 ttttctggct gatggcgcga tggtcttcac catgtcaaac atccactctg cggcggtcag
     3001 gtcttctgct gtcccccact tgctgccgct ctgaattgca gcatccggtt tcaccacagg
     3061 aagatcgttt tctggttggt cagaggattc gccagaattc tcggacgaaa aaggttttat
     3121 attgtctttt gttagtttgt cttttgtgtt tacctgattc gggtaaacgc ctttacctga
     3181 tttgggtaaa ctttttttac ctgattcagg taaatttacc tctttcaggt aaactttatt
     3241 tttcttacct gattcgggta atgttgacca ttcactgacc acattattaa tgccggtatt
     3301 ccgcccgctc tgaataaaaa tcccacgctt taccagaaca ctttttgcag cagaacactt
     3361 gtgcggcaat atcccggtta attcggaaag ttgctcgttg ctaacccaat ccagtttttt
     3421 attaaagcca tatgttttgc gcatgacagc cagaaagacc agaagctggt gctgtgttaa
     3481 tccggccagc atcacagctt ccagcaactc atttgcaatg cgcgtataac catcatcgag
     3541 atctgccacg cgcggctcct tttgtgccgc atccggcact ggaaaattga atatctcagc
     3601 agtgtttgcc ataattcctc ccgcaatgag tgtgttacga tttgcacctg aaagtcggtt
     3661 ctgttccagc agaccggctt tcgccatttc tgaacctgtc atatcgcccc cagcatggta
     3721 gtaaccatcg ccatcaatgg accagccaga tctgggtcca cacgaaacat cgacacaata
     3781 ccttcactaa tttccttcag tttctggtgg cgtggtgcgt tgagaatgac agcctgtttt
     3841 gcctcactga gttccttttc catttcagcc aacctagcca tgaagctatc ctgctcaacc
     3901 aggtaaccgc gatattccag cggtagtacc gccagaattg ccggggtcag ttcacgcacg
     3961 ttatttcggt atttttcaga atcgaatttg ttatcgagga agcggaacag cttctggcgt
     4021 gcacggctga catcatcagg gaaatcgatg gtgccgccgc cctgctcccg atactcattc
     4081 acaatgagtg tggcaacgac atcctgatta tctacagccg accaggcgcg gacggcatca
     4141 cggatttttt cgtggcctgg cacctgtttt gtttgagaac gatttatcac cgcagtcggg
     4201 ctaaatccgc tagtctgttg gtatgtaagt ggttgcataa ttgactcctt tagtttgaat
     4261 tgactgttaa gttgattgct tattgttaaa gagcgtgaaa tggaaattta agctgcgttc
     4321 ttttcggtgt gtggaaacaa cttcggaaga tccgggcgaa tctggtatgc cttcactact
     4381 ccaccagtag ccgtaacaat gctgccgaca tgttcagggg atacctttgc tttgttgtga
     4441 agccacttat agacggcctg ctgtgaaact tcgcaagcag cgcccagttt cttttgtgaa
     4501 ccaacgatat tgatcgctgt tttgatagct gggttcataa caacctccgt ggttaatttg
     4561 aatcaagatt aaaactatgg ttgtttttag tcaacaacca ttttcgtttg atggaataaa
     4621 accttggttg tacatttgga ctatgaaaac aacactctca gaaagactta aagaagccag
     4681 attagcgcga ggccttacac aaaaggcgct tggggatttg gtcggggtta gccaggctgc
     4741 tattcagaaa atcgaaacag ggaaagctaa tcaaacaact aaaatcgtgg agatcgcgaa
     4801 cgctttgggt gtgcgcgcag aatggttatc ttctggcgtt ggaaatatgt cagacagtac
     4861 agtgcaacca atacaatcaa ctgtcagcca ttccaaatac ttcaagattg acgttcttga
     4921 tatagaagtc agtgctgggc cgggagtcat caaccgtgag tttgtagaag ttctacgctc
     4981 ggttgagtac tcgtttgacg atgctcgtca catgttcgat ggtaggaagg cggaaaatat
     5041 ccgcatcatt aacgtgcgtg gtgacagcat gtcaggaacg atcgaaccag gtgatctgct
     5101 gttcgttgat atcacagtta aatctttcga cggtgatggt atctatgcgt ttctgtacga
     5161 cgacacagcc catgtaaagc gcctgcaaat gatgaaggat aagctgctgg tcatctctga
     5221 taacaaaagc tactcaccgt gggacccgat cgagaaagac gagatgaacc gggtgttcat
     5281 cttcggtaag gttattggga gcatgccgca gacatatagg aagcatgggt agtaccaatt
     5341 aaaaattatc aactgggcat tgtgctcatt cagtaaagaa ctaattccta tctttgctct
     5401 aggtagtaat attaagccac cgcaataata tctttaccta acggcgtaag aatcccggtc
     5461 accgtgccgg gttttctttt gccctcccct catcacacac accgttaaaa aaaccaccat
     5521 aacctcgctt cagttatcgc tatgcgattc aagtcacaaa ataaatccat cctaaataca
     5581 accagttata tctaaaacaa ccaataaaac aacttttgtt gttgacgata aaacaactat
     5641 agttttaaat aagttcatcg caacaacaca acgatacggc aactacctga ttcaccgttg
     5701 cgatgaccgc ttagatccgc agtttgaatt tcagcaggct tcggggagtg cgaggggtga
     5761 aacggacgcg tgaacgtcgg tgtgaccagc tgaaattaac tcaacatttc ataccttagt
     5821 cgcttcaacg aggcggctta gttatgacaa ccggcggcca tccaccgcct gaatacgcgc
     5881 agaagtctct atatgttcag cagcccagct tacgggcagg agtttttatg gttcatcaac
     5941 attatggaac gcagaccgtt aatcgaggtg cggtcatgcc aggaatgctg gtcaaacaca
     6001 aagatggtac ctggactgca tcagctaatt tacgcggacg gctttatctg catcgtggca
     6061 tcgagcgcac ttatacccgt gatttgctcg tggaagtttt tctcgacgga cgcggtaacg
     6121 gcctgaatca ctaatcccct ttcctgtttt cctaatcagc ctggcatttc gcgggcgata
     6181 ttttcacagc cattttcagg aggtcagcca tgaacgctta ttacattcag gatcgtcttg
     6241 aggctcagag ctgggcgcgt cactaccagc agatcgcccg tgaagagaaa gaggcagaac
     6301 tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc
     6361 atttgcaacg ccacggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg
     6421 agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccatc
     6481 aggttgatat tgattcagag gtataaaacg gatgagtaca gcactcgcaa cgctggcagg
     6541 gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct
     6601 tcgccagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat tactgatcgt
     6661 cgccaaccag tacggcctta atccgtggac gaaagaaatt tacgccttcc ctgataagca
     6721 gaacggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca atgaaaacca
     6781 gcagtttgat ggcatggact ttgagcaaga caatgaatcc tgtacatgcc ggatctaccg
     6841 caaggaccgt aatcatccga tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc
     6901 attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat
     6961 gttacggcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta
     7021 tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcag aacgtcagcc
     7081 ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca ctctgctgat
     7141 tgccctggat aaaacatggg atgacgactt attgccgctc tgttcccaga tatttcgccg
     7201 cgacattcgc gcatcgtcag aactgacaca ggccgaagca gtgaaagctc ttggattcct
     7261 gaaacagaaa gcctctgaac agaaggtggc tgcatgacac cggacattat cctgcagcgt
     7321 accgggatcg acgtgagagc tgtcgaacag ggggatgatg cgtggcacaa attacggctc
     7381 ggcgtcatca ccgcttcaga agttcacaat gtgatagcaa aaccccgctc cggaaagaaa
     7441 tggcctgaca tgaaaatgtc ctacttccac accctgcttg ccgaggtttg caccggtgtg
     7501 gctccggaag ttaacgctaa ggcgctggcc tggggaaaac agtacgagaa cgacaccaga
     7561 gtcctgtttg agtttacttc cggcgtgaat gttactgaat ccccgatcat ctatcgcgac
     7621 gaaagtatgc gcaccgcctg ctctcccgat ggtttatgca gtgacggcaa cggccttgaa
     7681 ctgaaatgcc cgtttacctc ccgggatttc atgaaattcc ggctcggtgg tttcgaggca
     7741 ataaaatcgg cttacatggc ccaggtgcag tacagcatgt gggtgacgcg aaaagatgcc
     7801 tggtactttg ccaactatga cccgcgcatg aagcgtgaag gcctgcatta tgtcgtgatt
     7861 gagcggaatg aaaagtacat ggcgagtttt gacgagatgg tgccggagtt catcgaaaaa
     7921 atggacgagg cactggctga aattggtttt gtatttgggg agcaatggcg atgacgcatc
     7981 ctcacgataa tatccgggta ggcgcgatca ctttcgtcta ctccgttaca aagcgaggct
     8041 gggtatttcc cggcctttct gttatcagaa atccactgaa agcacagcgg ctggctgaga
     8101 agataaataa taaacgggag gcggtatgca caaagcatct cctgttgagt taagaacgag
     8161 tattgagatg gcacatagcc ttgctcaaat tggagtcagg tttgtgccaa taccagtaga
     8221 aacagacgaa gaatttcata cgttagccac atccctttca caaaagctgg aaatgatggt
     8281 ggcgaaagca gaagcagatg agagagacca ggtatgacaa ccactgaatg catttttctg
     8341 gcagcgggct tcatattctg tgtgcttatg cttgccgaca tggggcttgt tcaatgacac
     8401 ctcagcaaga aaacgccctt cgcagcattg cccgtcaggc taattctgaa atcaaaaaag
     8461 ccagacagca ttttccggat aaaaacgtcg atgacatttg ccgtagcgta ctaaagaagc
     8521 accgcgaaac ggtaacgctg atgggattca caccgactca tttaagcctg gcgatcggca
     8581 tgttgaacgg cgtctttaag gaacggtgaa catgaaaagc aaaatcatca gggagctaca
     8641 ggctcctttt ttattattcg catttaccct caagcgtatt aaccaacaat tcagggatta
     8701 atgaaagatg gcagacatca ttgattcagc atcagaaatc gaagaattac agcgcaatac
     8761 agcaataaaa atgcgtcgtc tgaactacca gactgtatcc gcaactcatt gttgtgagtg
     8821 tggcgatccg atagatgagc gaagacgcct ggctgttcag ggttgtcgga cttgtgcaag
     8881 ttgccaggag gagatcgaac ttaagaacaa acaatgggga ctgtgatggc ctcaaagcag
     8941 caaatttcaa catcgtccaa ctgaggtgta aaaatgttca gaatcatttt tcctaacacc
     9001 tggtacgtcg accaccacgg cactccctgc aaaatcctgc gttctaccca caacaaagtt
     9061 cactacatcc gaaaaggcag aacatgtatc gccagcatgt tccgctttaa tcatgacttt
     9121 gaacctgtga ataaagctga tgcagatcgg atagcagaag agatcgaaac ggcagaacac
     9181 attaagaagt tacgtgacat gcgttcaaaa agcagaggta accatggaat catacagcct
     9241 cacactcgat gaggcctgtc agtttcttaa gatatccaga ccaaccgcca ccaactggat
     9301 acgaacaggc cgcctacagg caacacgtaa agatccaacc aagccaaaat ctccttacct
     9361 cacaacacgg caagcctgca ttgcggcgct tcagtctccg ctgcatactg tccaggtgag
     9421 cgcgggtgat ggcataacag aggaaagaaa atgtcactct tccgcagaaa tgaaatatgg
     9481 tatgcctcgt attcgctccc gggcgggaaa cgaattaagg aatctcttgg cacaaaggac
     9541 aagcggcaag ctcaggagtt gcacgacaag cgaaaagcag aactctggcg agtagaaaag
     9601 ctaggggatt tacctgatgt cacttttgaa gaggcctgcc taagatggct tgaggaaaaa
     9661 gctgataaaa aatctctcga ttcagataaa agccggattg agttctggct tgaacatttt
     9721 gagggtataa ggcttaaaga tatctcggag gcaaagattt actctgctgt aagcagaatg
     9781 cataacagaa agacgaaaga aatatggaaa cagaaagttc aggccgccat caggaaaggt
     9841 aaagaaccgc ctgtttatga accaaagcca gtatcaactc agacaaaggc aaagcatctt
     9901 gccatgataa aggccattct ccgtgctgca gaacgcgact ggaagtggct ggaaaaagcg
     9961 cctgtcatca agataccagc ggtcagaaac aagcgagtca gatggctgga aaaggaggaa
    10021 gcaaaacgcc ttattgatga gtgccccgaa ccactgaaat ctgtcgtcaa gtttgcgctg
    10081 gcaactggtc tgagaaagtc gaacatcata aatctggaat ggcaacaaat cgacatgcag
    10141 cgacgagttg cctgggtgaa tccagaagag agcaaatcaa accgcgccat tggcgtggcg
    10201 ctgaacgata ccgcctgtaa agtgttgcgt gatcaaatag gcaagcatca caaatgggtg
    10261 tttgtacata ccaaggcggc taagcgagca gatggaacat caacgcctgc ggtcaggaag
    10321 atgcgcatcg acagcaagac atcatggcta tcagcttgtc gtcgtgcagg aattgaagat
    10381 ttccgtttcc atgacctcag acacacctgg gcaagctggc tgattcagtc aggcgtccca
    10441 ttatcagtgc ttcaggaaat gggcggatgg gagtccatag aaatggttcg taggtatgct
    10501 caccttgcgc ctaatcattt gacagagcat gcgaggaaaa tagacgacat ttttggtgat
    10561 aatgtcccaa atatgtccca ctctggaatt atggaggata taaagaaggc gtaactgatt
    10621 gaattgtaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta gaaggtcgtt
    10681 gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt
    10741 atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca agtgaacgac
    10801 cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacacacac cgctgcgcgt
    10861 ctaaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc ctaacggaaa
    10921 attgtccgct cctatgagac tggtaactat gaaaccaacg tcggtgatca ttatggatac
    10981 tcatcctatc atcagaatgt ctattgaagt tctgttgcaa aaaaacagtg aattgcagat
    11041 tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc gtcctgttga
    11101 tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc tgaaaaggat
    11161 caaacaaatc cagagcacag tgaaagtgtt atttttatca tcgaaatcag aatgctttta
    11221 tgctggcaga gcgatacaag ctggtgctaa cggttttgtc agtaaatgca atgatcagaa
    11281 tgatattttt catgccgttc agatgatcct ctccggatac acgttttttc ccagcaaaac
    11341 gcttaactat ataaaaagca ataaatgtag tacgaatagt tcaacgatca ctgtgctatc
    11401 taatcgtgaa gtgaccatat tacgttatct ggttagcgga ttatctaata aagaaattgc
    11461 cgataagtta ttacttagca ataaaacagt tagtgcgcat aaatctaata tttatggcaa
    11521 gctaggtttg cattcaattg tagagcttat cgactacgcc aaattatacg aattaatata
    11581 atattaatta taattgatca taaatatcgc atccgctttc gccacacctg gccgaacacc
    11641 gctggctaac gctcgataat tggcaaaaaa gtttagtgtg acattgccat ttgcatctac
    11701 ttcctcagtc gggctcgcct cccccagtgc gagccgggag cgatcgctat tacgtaattc
    11761 gatggcgacg gtttgtgcca ttgcgggatc atccagagcc agcaggttgg tatcggatgc
    11821 cggcgttccc gtaaataaaa tcgcaactga acccggagga catccctcca gccgcaggct
    11881 aaaagggacg agtgccgtgg tatcgccagc gttcagtaat tgtgtcgtag gccatctgcc
    11941 taaatctacc gtcttatcaa tatccgctgt gtttacggta caggagaaat caacaacgtt
    12001 accgtgcaaa ttgatattaa tcgttcccaa agggtcagct gcccatccac tggaaatcca
    12061 cagtaacccg cagaaacagc taaagagtgc tcttctcatt ctccgtacct cattgataat
    12121 cgacgcgtaa ataccccagc gcgctaaacg gcccttcggt cggtttttga ccggtaatac
    12181 tgataggcca ggcgcgaagt gtgacattgg ctgccgcagc tgcatccaga cggaaaggaa
    12241 taacgctatt gagatcgtta ggcgtgatcg gcgtatcgtt ctgatcggcg acaataaaac
    12301 ctaaatcctg attgtccgac accatcgcct gaccagaaac ggcactggct tccagacgca
    12361 ttgttaaata agcctgcgca gcaacattcg tacatttgac cgcaatgctc ttggtttgcg
    12421 gcatgacacc agcaggtcga ttacccggcc ctgccgcact aaataacgat gcgccgatat
    12481 caccaaaatc aaattcaaca atctgcccgg catttaattc gcagttttgc ggtacttcaa
    12541 cccggccacc aaaactaatg gtataaacag gtgtaaccaa cggatcgtaa ggcgttgatg
    12601 tcacatacac ggtaaacatc acctgtctgg ggatctccac catgttaata aagggacgaa
    12661 taacctttaa acgaaatatt aaacgagaat ccatcacccc gaatggaagg ttttgcgaaa
    12721 cgttaggatc tgttcccatc agaatataat tttgcggggg gaactgaata tccatcacac
    12781 tatcaaccag actcatcgca cctaatagat aatcatttaa ttgcatatat ttataattat
    12841 cgatagtttc ctgaacaata aagttggtga cataactacg gtatgtataa ttcaccagcg
    12901 taccgggtgg acaaattgct gagacaccta cccagccgga tttttccggt aaaacaacaa
    12961 tctgccctgg ctggttattg gtgctattaa agatatttga gagatcatag tgttcatctt
    13021 ctgctacgcc agtactattt gcacaaacag tagcccagga tatgtttgcc agacacaaac
    13081 atgccatcgc caataataat ctgcagatta tcttcattga taatttcctt tatgagccag
    13141 gatgtgtgca aactgccgaa ataacagtga cggcttgctg taagctttgc ttcggcaata
    13201 catagtgcgc aatgcagcgt gagttcgcgc cgtctcccca ctgaaccagt aattcaccag
    13261 acaatggcgc accacttaaa taaacttgcc cgtcatcacc cacatactgg ttattccgat
    13321 actgttttcc cgtaccagtg atccaaacgg caccggtttt ccgccctggg taacggtaat
    13381 gagcgcccgc acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg
    13441 cacaacgctg ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag
    13501 cgcgatacgg ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc
    13561 ggttaaaatg ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa
    13621 aacattggta tcccctaaag gctggctcag cgttatgcca ttttcatgcc cgaccacacc
    13681 gccagacagc tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgacccccag
    13741 cgtagcgtaa gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt
    13801 atcaccctgg cttacgtgat aactcaggtt gtggccttcc agtaaggtcc cacctacacc
    13861 tgccagccag ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg
    13921 gtcgagcgca ttttcccggg tgtagcgacg tttggtcaga acattgaaag gcactgaaac
    13981 attaagtccg acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga
    14041 gagtgaataa ctgatgccaa cccagctgct ggtatacccc acctgatacc aggtatctga
    14101 atccgaagta ttccagtatt tttgatgggt accagagata taaagcgagc caaagtcatt
    14161 aagtgattgt gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta
    14221 attcacgatt atcggttcat cccgatgctc accgtcgtaa tcgtaatcat attcgtaccc
    14281 ctccattcga cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta
    14341 acccattaac tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat
    14401 ggaatccccc tcatgacgac tgtcgtcggc taactgactg cgcgcatggg tcacatccag
    14461 cgacactgcc ccccagtttc cgagattgcg ccccagtcct aataaaaagg cggtgtaatt
    14521 ggcagataat tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt
    14581 gccctgaaaa aagaaaggcg atgattgctg actattaccg ctacgaaaat cgcccgccgt
    14641 caggtcaaat ttgaaacgcc cttcgcgttg taaaattggc actgttgaat acgggatagt
    14701 gtaattctgc tgattaccat cgcgctcgtc gatggtgacg tccagatcgc catttgaaga
    14761 tgtcgggtgc aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat
    14821 aaaaccattt tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg
    14881 ggcaaaccct tgctggctat caggatacat attatcggaa gaataaagcc gtacaccacg
    14941 aaatccaaca ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact
    15001 tttcagcgga ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg
    15061 atatccattt ccgcgaaaat agttccagga accattgttg cgtaaacgcc acgggccaat
    15121 attaatcccg ctgagctcac taaaaaaata gctatcgtta ccattacctc tgttaccggt
    15181 gaaattataa ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta
    15241 accgtgagcg ctactcaaca acgttatctg aggaatagtg atgttcaggc gcatcgctgc
    15301 aaaatcaaag ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt
    15361 ttgctgctgg gctaactcgg gaaatgcact gctattaagg ccaattcgtt caaggagtac
    15421 ctggttaaaa cagggcatta acccacctga tttatcacct gtattttctg ttgtcgattc
    15481 aaatacgata tcctgtgaac caatgaactc atcattacgc cagagatcca cccgatacac
    15541 tcccgcaggt tgatgattgc ctctctcaaa gcgcgataaa tcagcaacgg atgtgccatt
    15601 ctctaataaa aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat
    15661 ggcaagagat gaataacaaa ttccggccag acaccaggta tacctctgcg gaatatccgt
    15721 agtagtgggt attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt
    15781 cacttctctg accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc
    15841 ctgcactcct gagggtaagg gaatttgtga actaactctg ggtgccgcca tagcattccc
    15901 caacttttgg ctgcccactt gcaggttaac gagggtgaca taaaaaggtg atggattatg
    15961 aacattgata tagttaccgg atcgcgaaaa cttgagtgtg tccggcgctt ctgcgggtaa
    16021 ttcttgtaat tgaattggac ggagaaataa tttcatgcgc gataaaatcg ccagttgcaa
    16081 aacattcctg ccgttcaatg catttttatc taccgaaggg atcgtcttaa cattcatcca
    16141 gaacagagac tcacgatctg ctgccagcgg tggaccggtg taaataatac gcaaagtatt
    16201 ttcgcttttg ggttcactaa caaacagtgg cggtgtaatg atgaatgact tttcttttac
    16261 accgctgctg ttttcaatcc acgaattgac cagaaaacgc tcattggtat ggctatttct
    16321 aatccatacc gcagtctgtt tagcatcagc gggataaata atacgcgtgg cacctaatgc
    16381 gatccctccg gcagcatggg cgctggccga aatgattaaa taaaatataa tgagcatcaa
    16441 taactttatt ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag
    16501 aaatataaag aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata
    16561 aagcggcatg atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga
    16621 caaccggtac attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga
    16681 ttattcgtat ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt
    16741 accggtgcct ttataacgtg cagaaaaatg aagaacgttg gttccgggga tcaagttctg
    16801 gttggttgag aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc
    16861 aataccgaca cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc
    16921 attgattgca tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc
    16981 gcagtcgttc aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa
    17041 aatatcggta cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga
    17101 acaggcagca ttcaccagtt cgccttcaaa gtgaattgta ccgccatcta caacggcagc
    17161 ataactaccc gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt
    17221 catatatatt ccttcaatct cat
//
LOCUS       DAZFFI010000093        17093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFFI010000093 DAZFFI010000000
VERSION     DAZFFI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.093"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..724
                     /gene="ais"
                     /locus_tag="ACHJ12_003661"
     CDS             122..724
                     /gene="ais"
                     /locus_tag="ACHJ12_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4991391.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHJ12_003662"
     CDS             complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHJ12_003662"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4991392.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHJ12_003663"
     CDS             1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHJ12_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4991393.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHJ12_003664"
     CDS             2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHJ12_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4991394.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3375..3836
                     /locus_tag="ACHJ12_003665"
                     /pseudo
     CDS             3375..3836
                     /locus_tag="ACHJ12_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3877..4779)
                     /gene="rpnE"
                     /locus_tag="ACHJ12_003666"
     CDS             complement(3877..4779)
                     /gene="rpnE"
                     /locus_tag="ACHJ12_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4991395.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4973..6163)
                     /gene="glpC"
                     /locus_tag="ACHJ12_003667"
     CDS             complement(4973..6163)
                     /gene="glpC"
                     /locus_tag="ACHJ12_003667"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4991396.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6160..7419)
                     /gene="glpB"
                     /locus_tag="ACHJ12_003668"
     CDS             complement(6160..7419)
                     /gene="glpB"
                     /locus_tag="ACHJ12_003668"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4991397.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7409..9037)
                     /gene="glpA"
                     /locus_tag="ACHJ12_003669"
     CDS             complement(7409..9037)
                     /gene="glpA"
                     /locus_tag="ACHJ12_003669"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4991398.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9310..10668
                     /gene="glpT"
                     /locus_tag="ACHJ12_003670"
     CDS             9310..10668
                     /gene="glpT"
                     /locus_tag="ACHJ12_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4991399.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10673..11749
                     /gene="glpQ"
                     /locus_tag="ACHJ12_003671"
     CDS             10673..11749
                     /gene="glpQ"
                     /locus_tag="ACHJ12_003671"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4991400.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11791..12032)
                     /locus_tag="ACHJ12_003672"
                     /pseudo
     CDS             complement(11791..12032)
                     /locus_tag="ACHJ12_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12027..12131
                     /locus_tag="ACHJ12_003673"
                     /pseudo
     CDS             12027..12131
                     /locus_tag="ACHJ12_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12211..12861
                     /gene="inaA"
                     /locus_tag="ACHJ12_003674"
     CDS             12211..12861
                     /gene="inaA"
                     /locus_tag="ACHJ12_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4991401.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12915..13169)
                     /gene="yfaE"
                     /locus_tag="ACHJ12_003675"
     CDS             complement(12915..13169)
                     /gene="yfaE"
                     /locus_tag="ACHJ12_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4991402.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13169..14299)
                     /gene="nrdB"
                     /locus_tag="ACHJ12_003676"
     CDS             complement(13169..14299)
                     /gene="nrdB"
                     /locus_tag="ACHJ12_003676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4991403.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14388..16673)
                     /gene="nrdA"
                     /locus_tag="ACHJ12_003677"
     CDS             complement(14388..16673)
                     /gene="nrdA"
                     /locus_tag="ACHJ12_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4991404.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16801..16944
                     /gene="ypaB"
                     /locus_tag="ACHJ12_003678"
     CDS             16801..16944
                     /gene="ypaB"
                     /locus_tag="ACHJ12_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4991405.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4036 a         4178 c         4574 g         4305 t
ORIGIN      
        1 ttgaggcatt ttgccgacat cgtaggattt ttaatattac accaactgct aattatcgcc
       61 agaaatgtcg ctcaatttta cggtaattgt ctggttgcgc ttgtctatag gtggagttta
      121 cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc
      181 gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg
      241 tatcgacaat aaaacattgg ccagactggc acagcagcac ccggttgtcg ttttgtttcg
      301 ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac
      361 ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagcgctg acatccctga
      421 tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct ggttttccgc
      481 gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc
      541 aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca
      601 ttgcctgaca tatattgcta aaaataagcg tgatgcgaca tttaaacctg attatctgga
      661 tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaacca
      721 ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa
      781 acgtaacgtt tttcgggtgg caacatttaa atcataatgc accagatctt caggttttac
      841 ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcagtc
      901 aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg tcttggtgcg
      961 aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc
     1021 ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccagactcca cgccgccacc
     1081 cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata
     1141 agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcatag gggtattcct
     1201 tataaataaa aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa
     1261 tctcttttgc aatgataact gcacttatta ttatttttgt gtgtatcggt aaagtgtgtt
     1321 tattttttcg gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct
     1381 tttttcgctc attgaatcct ctgtgccagg cagtacaatc ccgcagcttt gggaattttg
     1441 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
     1501 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
     1561 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
     1621 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
     1681 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
     1741 atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
     1801 gtttgtttgg cgagtattac tactctccgg attcgctctc cagcggtatt caaagttatg
     1861 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
     1921 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
     1981 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
     2041 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
     2101 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
     2161 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ctgccattat
     2221 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
     2281 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
     2341 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca
     2401 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     2461 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     2521 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     2581 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     2641 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     2701 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcttca atgccgatta
     2761 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     2821 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     2881 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     2941 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg tgtgaagtgg
     3001 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgggcca
     3061 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgaacat ctcaactttg
     3121 cgctagccac accagacggc actttcgctc tgcgtgtgcg tttcagcact acgcgctaca
     3181 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgctg cgccgttggt
     3241 taaacggcca ggatatcgcc agtgagcatg gctggattga ggttattgag tccatgacct
     3301 tatctgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     3361 aaaacttgtc caaccctccc tacggtgagc gtggtgttgg ggccagtgtg gcacgggctg
     3421 cgcgctgggg acgcattgag aattacatgg cgcaagtcaa cgattcgctt tgtctgttag
     3481 tgcaggtgga aagtaaaacg gcactggata acctggacga gatcctcgac gtcgaaggga
     3541 ttgatggcgt gtttattgga ccagcggatc tttctgcgtc gttgggctac ccggataacg
     3601 ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc cgtgctgcgg
     3661 gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc ctggcgtggg
     3721 gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc ctggatcaac
     3781 gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt tattgatatc
     3841 aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg ctgccagatc
     3901 ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga tcaatgccct gttccagcat
     3961 gcgcaaagca attttaatgg cttgttcatg cataccttcc tgccagccaa tttgatgccc
     4021 ttcctgccgt agccgttcag caatagtcat taatctctcc ttttgtagtg gtgaacgttc
     4081 ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg ccgcattgca gcagataatt
     4141 aaacagtgtt tgtagctggc tgtcattagt gaagcctcta accaaaagcg tggtgatcct
     4201 gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt tgaatcagtt ccagcagagc
     4261 tatacgccga tgttgcatga tctcatcgtc aggtacgatg gtgatatcca ccaacggaaa
     4321 agcttcggtg tacaactgcc gggcaagttg cggatcgtca aactcatcca gccagttagg
     4381 tgagtacggg tagggcgatg tttcgccatg ataaaacagc aatggcacca ccagcggaac
     4441 tctgtcatag cctttatcca ggtgacgctg catggcggca gtggcatagc gcattagccg
     4501 aaaagccata ttcttttctg cagagctttg atgttcaatc acgcagtaga tatagccgtc
     4561 accgtcgctg gtttccacgg accacaaaac atccgagtag taagcgcgta aacttttttc
     4621 aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg caaagcttgc gcagtggttc
     4681 tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt gtgaacataa aggttttaaa
     4741 caccgcatca tgcggggagg atgttgttga ttcggtcatg gttgtccgtc acctgatatt
     4801 tatgatgacg cgacattacg tctatcccgg gcctttgaca tctgtcagtt tcagcctttg
     4861 cgagacattt tcagaggaat tttcaaagcg ttgcaacgca tacatggtgc gtggaatcgc
     4921 gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca tcaaaaggtc ggttaagcca
     4981 gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag gctcgtggac atctcaatct
     5041 gccatttgca ggtttcgcag tcggtgatga ccagatccgc gccgctctct tctatctggc
     5101 ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt ctcttttttg aaaccgtaag
     5161 tacccgcaat accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggattttac
     5221 gcaacagctc cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat
     5281 aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc
     5341 acagccagcg ggttgccagt tcgatatgat cgcgcaagcc tttgttgtcg acattcagca
     5401 cttccgggta ttcgtcgcgc agggcaaatg tacaggttga ggaggtggca atcaccggaa
     5461 tgccttttac tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg
     5521 ctttatcggt aaagccgttg gcgattagcg gtacgccgca gcatttttct ttgctgagca
     5581 gttgtacacc ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat
     5641 ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg gtctttatat tgtgcttgct
     5701 gagccgctat gctgcgatac cagcgacgga acgtgccgaa ggagtatttc ggtagcgtgc
     5761 ggcgatgatc gatttttaac gccgcatcaa gcagctgccg caccggtttc agcgaggtag
     5821 cggtgttgac gattggtgcg aacggcgtgg aaacgctacc catcaggtcg gtatgactca
     5881 acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt tgcccgcgcg cgctggataa
     5941 tatcgccaat cttcacatcg gacgggcagg cgacttcaca acgtttgcag ttgatgcaat
     6001 atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg cagacgctcg ccatccggcc
     6061 cggcttgttt tggccctgga taaccgggat tcacccggct caccgggcag gcggtggtgc
     6121 agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt cattgttggc ctcctgcgcg
     6181 ttgggcaatc tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc
     6241 ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt
     6301 ttgccctgcc tgcgacgggc gtagcgtctc atcagtggtt acaccgaact gctgccacgg
     6361 ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc gtggcggttt gtagcacatc
     6421 aaggccgaga atcggctcgc gaatgccgtt acgttccgct accagtccgc cactaaagaa
     6481 actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga atatcggcgt gattgcgggt
     6541 ccagatttca ttcactacgc catttttaca ggtcactttt ttcacttcat cgcccggcat
     6601 ccacacgcca ccctggcgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag
     6661 cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca
     6721 acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt
     6781 attggcgaca ggaataagcg catcaagtaa cagcggccag ttttcttcat tatcaaggaa
     6841 acgggcgata ttcacagcgc gaaattcggt ggcgttattg cgcaggacat ccagttccgg
     6901 cagctctatt tctgcggttt caaccgaaag atccagttcg cgcaacgaag ccgccgcaag
     6961 atgcgcctgg aaatccatca gcccgcttat ccccactaca catatcttct tcgcgggtag
     7021 cggccagacg ggaacttctg gcgaacttaa ccaggttgca cgcaaagtgc cgagcggggt
     7081 aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc gcaccgcttt cagcgatcaa
     7141 tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt tcgagaagac tgtaaggatg
     7201 ggctggtgcc tgctgacgca aagattccag tccactgtga atgtctgtca ccggttgacc
     7261 atcaggcaga tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc
     7321 acgcgtgaca atggcacagc gcaggccgtg tttttgcagt tgcaggccac agagtaatcc
     7381 ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc tttctgctcc ttctccagac
     7441 cacacagtcc ctgataaacc cagcgggtaa attcgctttc gcgcagtgcg tctccccagg
     7501 cgatgggttg cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt
     7561 gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc agcgcggcag gcgcagagtt
     7621 cgccctggca ggtacccatc cccacacggg tacgacggcg taaatccagc aggctattaa
     7681 cgtttaaatt ttctaccgcg tactgaactt cacccgcagt taccgcttcg cactcacata
     7741 ccaggctacg atgcagacgg ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat
     7801 aaaccgcaga accgcgcagc ggggcaggca gggagatgac tttacgcaag gtaacttcag
     7861 cgggttcttg tgaaccaggc agtgccagat cggcagtcgt acaggggcgc gtgttgccca
     7921 gtttgcggca taccgcgtcg gtagcccatt cagccatcag ccgataggtc atcagtttgc
     7981 caccggtgat ggtgataaat ccgtccagac catcgcgttc agcatggtcg agcagcacga
     8041 tgccacggct gacgttacgt ccgctcgggt cgtcatcgct ggcaaccagc gggcgcacgc
     8101 cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac
     8161 gcagcagaat atcaacctct tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa
     8221 tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt
     8281 cggaaggttt acggcagcgg ttgatcacat gctggttaat gcggtgatcc atgatcagca
     8341 gcgatccttt cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc
     8401 cccagatccc agcggcatta accacgacag gtgcatgaag ggcctgagtt tcgccggtga
     8461 gatggttacg tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt
     8521 catgagcggt aaggataacg gcaccgtgtt ctttggcatc cagcatgttt gctgcggtca
     8581 gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa
     8641 cggcaggttc gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt
     8701 cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc atcttccggc agggtgataa
     8761 acaggccgtt ggttggttca acgcagtgac gtgcaatgcg tttcaggatc tggttttcac
     8821 taatgcattc gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca
     8881 ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc
     8941 gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc cgttgcgccg ccaccaatga
     9001 taatcacgtc acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt
     9061 tttaccattt agccatagta aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa
     9121 ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat tttgtaaatc ttatttaata
     9181 atgtgtgcgg caattcacat ttaatttctg aatgttttct taacatcgcg gcaactaaag
     9241 aaacggcagg ttctcccact gaatcagtct gttaatcata aataagacca cgggccacgg
     9301 aggctttcaa tgttgagtat ttttaaacca gcgccacaca aagcgcgctt acctgctgcg
     9361 gagatcgatc cgacttatcg tcgattgcgc tggcaaattt tcctggggat attctttggc
     9421 tatgcggctt actatttggt tcgtaagaac tttgcgcttg ctatgcctta tctggtcgag
     9481 cagggattct ctcgcggtga tttaggtttc gcgctttcgg ggatctcgat cgcttatgga
     9541 ttttcgaaat tcataatggg ttcggtatca gatcgctcga atccgcgcgt tttcctcccc
     9601 gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg
     9661 tcgagcattg cggtgatgtt tgtacttttg ttcctctgcg gttggttcca ggggatgggg
     9721 tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc agaaagaacg tggcggcatt
     9781 gtgtcagtgt ggaactgtgc gcacaacgtt ggtggtggta ttccgccgct gctgttcctg
     9841 ctggggatgg cctggttcaa tgactggcac gcggcgctct atatgcccgc tttctgcgcc
     9901 attctggtgg cattattcgc ctttgcgatg atgcgcgata ccccgcaatc ctgtggcttg
     9961 ccgccgatcg aagagtacaa aaatgattat ccggacgact ataacgaaaa agcggaacag
    10021 gagctgacgg cgaagcaaat cttcatgcag tacgtactgc cgaacaaact gctgtggtat
    10081 atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg
    10141 acttatctga aagaggttaa gcatttcgcg ctggataaat cctcctgggc ctacttcctt
    10201 tatgaatatg caggtattcc gggcactctg ctgtgcggct ggatgtcgga taaagtcttc
    10261 cgtggcaacc gtggggcaac cggcgttttc tttatgacac tggtgaccat cgcgactatc
    10321 gtttactgga tgaacccggc aggtaaccca accgtcgata tgatttgtat gattgttatc
    10381 ggcttcctga tctacggtcc tgtgatgctg atcggtctgc atgcgctgga actggcaccg
    10441 aaaaaagcgg caggtacggc agcgggcttt accgggctgt ttggttacct gggcggttcg
    10501 gtggcggcga gcgcgattgt tggctacacc gtggacttct tcggctggga tggcggcttt
    10561 atggtaatga ttggcggcag cattctggcg gttatcttgt tgattgttgt gatgattggc
    10621 gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg gaggctaatg gcatgaaatt
    10681 gacgctgaaa aaccttagca tggcgatcat gatgagcact atagtcatgg gaagcagtgc
    10741 aatggcggcg gacagcaacg aaaaaatagt catcgcccat cgcggtgcca gtggatattt
    10801 gccggagcat acgctgccag caaaagcgat ggcgtatgcg cagggagcgg attatctgga
    10861 acaggatttg gtgatgacca aagacgacca tctggttgtt ctgcatgacc attatctcga
    10921 tcgtgttact gatgttgccg atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta
    10981 cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat
    11041 tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc ccaatgggta agtccgactt
    11101 ccgggtgcac acctttgaag aagagattga atttgttcag gggttaaatc actctaccgg
    11161 gaaaaatatc ggtatttatc cagaaatcaa agcgccgtgg ttccatcatc aggaagggaa
    11221 ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat ggttacaccg gtaaagacga
    11281 taaagtttat ttgcaatgtt ttgatgctga tgagctgaag cgtattaaga atgagctgga
    11341 acccaaaatg ggcatggagc tcaatctggt acagctgatt gcctataccg actggaatga
    11401 aacgcagcag aaacagccgg atggaagctg ggttaattac aactacgact ggatgtttaa
    11461 gccgggtgcc atgaaacagg tggcggaata tgcagatggt attggtccgg attaccatat
    11521 gttgattgag gaaacatcgc agccgggtaa tatcaaactc actggcatgg tgcaagatgc
    11581 tcagcagaat aaactggtag tgcatcctta taccgtgcgg tcggataaac tgcctgaata
    11641 cactactgat gtgaatcagt tatatgatgc tctgtataac aaagcgggtg taaatgggct
    11701 gtttactgat ttccctgata aggcagtaaa attccttaat aaagagtaat gctactgcag
    11761 gatggcgttc atgcgccatc ctgtctcata tcaaattttt ccattcacca cgagttgttc
    11821 cgtacacgtt tgtagtaaaa acagtggact gccttctacc ggcttttctt tagccaacaa
    11881 cgaaatctgt cgatagaagg ttggttcgtg aggaacggac acaattcacc tgcaatgctt
    11941 ttcagcgtta actctggttg taaggcgatg cctaatccct gtcgaataaa attcatcgct
    12001 gtatctgact gattaaattc acattttatc tgctgggcgc actattagtg gtagtggctg
    12061 gtggaatcta tgcgctgcaa atgaagggca aaagcggtgt cacacctgta gcggcaaaag
    12121 aaattcatta atacgacacg tttcattaag attttcctca ggtaaccacc tatagtcatt
    12181 gtcgttaata aaaatgacag gcgatagggt atggcagttt cagcaaagta cgacgaattt
    12241 aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg ccgtaatggt
    12301 atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa gcgcatgacg
    12361 catcatctgt ttcattctgt acgttatccg ttcggccgac caacgattgt ccgtgaggtt
    12421 gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt ttttggtgaa
    12481 gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga ctgaagatat ggcggggttc
    12541 atcagcattg ctgactggta tgcccagcat gcagtatcgc cttattctga cgaagtacgg
    12601 caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcat taatcgtcag
    12661 catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaaggc agaagctggt
    12721 tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat taatcatgac
    12781 ttccgccagc tggaaaaata tctggagcca atccccaaag cggactggga gcaggtgaaa
    12841 gcgtattact acgccatgta atcgttgtag gccagataag acgcataagc gttgtatctg
    12901 gcactacaac caattcacat ctcgatttca atatcgcctt ttgcccggca acaacagggc
    12961 aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtcaac ttgacctgca
    13021 accaggcgtg tgcgacagga gccgcagtaa ccttcgcgac actgatactc aaccgccaca
    13081 ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag cagttgtgtg
    13141 ccagtgatgc gcagggtaac gcgggccatc agagttggaa gttactcaaa tcgtcggtgt
    13201 ccacttccgc gtcaatctgc ccgaccagat aagaactgac ttccacttcc tgcggagcaa
    13261 cctgcacgtt atcagacacc agccaggtgt tgatccatgg gatcgggttg gagcgcgtct
    13321 ggaacggcag atccaaaccg actgcctgca tacggatatt ggtgatgtat tcaacgtact
    13381 ggcagagaat atctttattc agaccaatca tcgaaccgtc acggaacaga taatccgccc
    13441 agtctttctc ctgttgagct gcctgaacaa acaggtcata gcactcctgc ttacactctt
    13501 cggcaatttc cgccatctca ggatcgtccg cgccgctgcg cagcagattc agcatatgct
    13561 gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt ttggcgttgc
    13621 cttccatcaa ttcgcgttct gcaaaggcaa aggaacaagc aaagctgacg tagaagcgga
    13681 tcgcttccag cgcgttaacg ctcatcaggc aaagatacag ttttttcttc agctcgcgca
    13741 ggctaacggt tacggtttta ccgttaacgg tgtgggtgcc ttcgcccaga aggtgccagt
    13801 agctggtcat ttcgatcagt tcatcgtaat agctggagat cccttccgca cgtttctgga
    13861 tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg atcgttaacg atattacgaa
    13921 tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt tcgacccagg
    13981 tttccagttc cggaatagaa ataagcggca atagcgccac gttcggacta cgaccctgaa
    14041 tggagtccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt tcgtgctccg
    14101 gcagcgcctg gtaatcgata cggtcgcggg agacgtcaac ttcttccgga cgccagaaga
    14161 aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga tcgtagcgag
    14221 ccacgttgac cggctgacca aaaaacatcg gttctttaag ctgatcattt ttcgtctgtg
    14281 aaaaggtggt atatgccatg agtgtgtcct gtggttttat tacccctcac cctaaccctc
    14341 tcccgcaagg ggagagggga cggtttggtg caaaggctgg gggattatca gatcttacat
    14401 gcgccgcttt cgcagccatc gtcctggatt gacggcacca gatcgtcctg cgcgtcttca
    14461 gcgccgtcac gggtgttctg ataatacagc gttttgaccc cgaatttgta ggcggtgagc
    14521 aggtctttca gcaactgctg catcggcact tttcctgacg ggaaacgtga cggatcgtag
    14581 ttggtgttag cagagatcga ctgatcgata aatttctgca tgatacctac cagttgcagg
    14641 tagccatcgt tacccggcat ttcccacagc agctcatagg cgtcgtgcag gtgctcgtag
    14701 tccggcacca cctggcgcaa aataccgtct ttcgacgctt tgatgctgac gtaaccgcgc
    14761 ggcggttcaa taccgttagt ggcgttagag atctgcgaag aggtctcgga cggcatcagg
    14821 gcagaaagcg tggagttacg caggccgtgc gttttgattg attcacgcaa tgcttcccag
    14881 tcgtaatgca gcggctcatt agcgatggca tccagatctt tcttataggt atcgatcggc
    14941 aggatccctt tcgcgtaagt ggtttcgtta aaccacgggc acgcgccttg ctctttagcc
    15001 agctcattag acgctttcag caggtaatac tgaatcgctt cgaaggtttt atgcgtcagg
    15061 ttgttggcgc tgccgtcgga gtagcgttta ccgtgcttcg ccagatagta agcgaagtta
    15121 atcacaccaa tacccagcgt acgacgcccc atcgctccac gtttggcggc cgggatcggg
    15181 taatcctgat aatccagcag cgcgtcaagt gcacgaaccg ccagaattgc cagctcttcc
    15241 agttcatcca ggctattaat tgcgcccagg ttgaaagcag acagcgtaca cagcgcgatt
    15301 tcaccgttct cgtcgttgac gtcgttcagc ggtttggtcg gcagggctat ctccaggcac
    15361 aggttagact gacgcactgg cgcgatggcc ggatcaaacg ggctatgggt attgcagtgg
    15421 tcaacgttct gaatatagat acgaccggta gacgcacgtt cctgcatcat cagcgagaac
    15481 agctcaacgg ctttcacacg ctgcttgcgg atgctgtcgt ctttctcata tttggtatac
    15541 aggcgttcaa actcttcctg atcggcgaag aatgcgtcgt acagccccgg tacgtcggac
    15601 gggctgaaca gggtgatatc ttcacctttc agcaggcggg tatacatcag tttgttgatc
    15661 tgtaccccgt agtccatatg gcgcacgcgg ttgccttcca caccacggtt gtttttcaac
    15721 accagcaggc tttccacttc cagatgccac atcgggtaga acagcgttgc cgcaccgccg
    15781 cgcacgccgc cctgagagca ggatttcacc gctgtctgga aatgtttgta gaacggaata
    15841 cagccggtat ggaacgcttc accaccgcga atcgggctac ccagcgcacg aatacgcccg
    15901 gcgttgatgc cgatcccggc acgctgggaa acgtatttca caatcgcgct ggaggtagcg
    15961 ttgatggaat ccagactgtc accgcactcg atcagtacgc aggagctgaa ctgacgagtc
    16021 ggggtacgca cgccggacat gattggcgtc ggcagcgaaa ttttaaatgt ggaaaccgcg
    16081 tcgtaaaaac gcttcacata ttgcaggcgc gtttcacgcg ggtagttcga gaacaggcac
    16141 gcggcaacca gaatataaag gaactgggcg ctttcgtaga tttcgccggt cacgcggttc
    16201 tgtaccagat atttgccttc cagctgctta acggcagcat aagagaaggt catatcacgg
    16261 tcgtgatcga taaacgtgtc catctgcttg aactcttctt ccgtgtagtc ttccagcaga
    16321 tgattatcgt atttgcccat ctcgaccatt ttcaccacgt ggtcgtacag cgcaggcggc
    16381 tcaaactggc cgtaggcttt tttacgcagg tggaagatcg ccaggcgcgc ggcgagatac
    16441 tgataatccg gcgcatcacg ggagatcagg tctgcggccg ccttgataat ggtttcgtgg
    16501 atgtcagagg tcttgatacc gtcataaaac tgaatgtggg agcgcagctc gacctgggaa
    16561 atcgaaacgt tatgcagtcc ttctgccgcc caatccagaa cgcgatggat tttgtcgaga
    16621 ttgatgcgct ctgtgctacc gtcgcgcttt gtcaccagca gattctgatt catgtatgtc
    16681 gtacctgttt ttggaattct ttccgcaata gtttcatgca gactacctgt agtaagggaa
    16741 gctgcttaga acactatata tagggggtat gtttgggatt cactgcaaga tagtgtgaaa
    16801 atggccctct tgcaagtgca taactttgtg gataactcag gaaggaaaaa gtggctttcg
    16861 cgcaccttag gtcagacaag gtgtccggga aagtcaatgg gaagagaaaa atttgttaaa
    16921 aataactgtt cggcgatttc ttgaacggca gaataggttg gataaattga tatagatggt
    16981 ttattaatct ttttgctaat gattgcaaaa attgatctaa gcacgagtca tcacataatc
    17041 tgatgtatca gttgtggtca atatataaac gaatgtaagc gattgttgat gtc
//
LOCUS       DAZFFI010000094        16640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFFI010000094 DAZFFI010000000
VERSION     DAZFFI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.094"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(146..682)
                     /gene="yghD"
                     /locus_tag="ACHJ12_003679"
     CDS             complement(146..682)
                     /gene="yghD"
                     /locus_tag="ACHJ12_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO4991406.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMVARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(684..1862)
                     /gene="gspL"
                     /locus_tag="ACHJ12_003680"
     CDS             complement(684..1862)
                     /gene="gspL"
                     /locus_tag="ACHJ12_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO4991407.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMPNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1859..2836)
                     /gene="gspK"
                     /locus_tag="ACHJ12_003681"
     CDS             complement(1859..2836)
                     /gene="gspK"
                     /locus_tag="ACHJ12_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO4991408.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLRLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2833..3438)
                     /gene="gspJ"
                     /locus_tag="ACHJ12_003682"
     CDS             complement(2833..3438)
                     /gene="gspJ"
                     /locus_tag="ACHJ12_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO4991409.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3435..3806)
                     /gene="gspI"
                     /locus_tag="ACHJ12_003683"
     CDS             complement(3435..3806)
                     /gene="gspI"
                     /locus_tag="ACHJ12_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO4991410.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3803..4366)
                     /gene="gspH"
                     /locus_tag="ACHJ12_003684"
     CDS             complement(3803..4366)
                     /gene="gspH"
                     /locus_tag="ACHJ12_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO4991411.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4370..4825)
                     /gene="gspG"
                     /locus_tag="ACHJ12_003685"
     CDS             complement(4370..4825)
                     /gene="gspG"
                     /locus_tag="ACHJ12_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO4991412.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4842..6065)
                     /gene="gspF"
                     /locus_tag="ACHJ12_003686"
     CDS             complement(4842..6065)
                     /gene="gspF"
                     /locus_tag="ACHJ12_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO4991413.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6065..7558)
                     /gene="gspE"
                     /locus_tag="ACHJ12_003687"
     CDS             complement(6065..7558)
                     /gene="gspE"
                     /locus_tag="ACHJ12_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO4991414.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKSHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7558..9618)
                     /gene="gspD"
                     /locus_tag="ACHJ12_003688"
     CDS             complement(7558..9618)
                     /gene="gspD"
                     /locus_tag="ACHJ12_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO4991415.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9648..10607)
                     /gene="gspC"
                     /locus_tag="ACHJ12_003689"
     CDS             complement(9648..10607)
                     /gene="gspC"
                     /locus_tag="ACHJ12_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO4991416.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10625..11035)
                     /gene="gspS2"
                     /locus_tag="ACHJ12_003690"
     CDS             complement(10625..11035)
                     /gene="gspS2"
                     /locus_tag="ACHJ12_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO4991417.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11101..11910)
                     /gene="pppA"
                     /locus_tag="ACHJ12_003691"
     CDS             complement(11101..11910)
                     /gene="pppA"
                     /locus_tag="ACHJ12_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO4991418.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(12050..16615)
                     /gene="sslE"
                     /locus_tag="ACHJ12_003692"
     CDS             complement(12050..16615)
                     /gene="sslE"
                     /locus_tag="ACHJ12_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO4991419.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPTEIIL
                     SEGRLVDSQGDGVVGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLS
                     NGATLGEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKS
                     SMTVGTNLETVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNG
                     FEYVTQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTID
                     DTTKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQ
                     RILDAFPGLEVCKDSDYHYEVNCLEYRPGTDVPVTGGMYVPQYTQLDLSADTAKAMLQ
                     AADLGTNIQRLYQHELYFRTNGRQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKED
                     ELGFKTFTEFLNCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGVVNTNGETVTQNINLYSAPTKWFAGNMQSTG
                     LWAPAQQEVSIESKATVPVTVTVALADDLTGREKHEVSLNRPPRVTKTYDLKANDKVT
                     FKVPYGGLIYIKGDSKEVQSADFTFTGVVKAPFYKDGKWQHDLNSPAPLGELESASFV
                     YTTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDSEDGKHRMFTYKNLPGHKHR
                     FANDVQISIGDAHSGYPVMNSSFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDEVSNDKFGGKNYC
                     AESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNT
                     LASLNLPKPKQGPETINKVTEYSMPAE"
BASE COUNT         3651 a         4737 c         4217 g         4035 t
ORIGIN      
        1 attcttaaga acccgcccac aaggcgggtt tttgcttttg gatctgacaa taaccttcac
       61 gaaaaaagtt tgcttataaa gtctggggga attactctcg ccacgttaac gagagtaatt
      121 ttattgatac taatctcctg atactttacc cccgtccaaa ctccagccgc tgcacattca
      181 ccatcccagt cttctcagca gcactggcat caatttgtgt cacccgcagc gcatactttt
      241 catccagcgc gtttaaccag ttcagcaggt cattaaacac caccggatct atccagacct
      301 gaatattctc cccacgctcg gctatccgct tgatgactac cgagtgcgcg gaagcgctgt
      361 cactgataac ccgcgatacc tgcgcaggcg ttgttgtggc ggattttcgc gccaccataa
      421 tatccggcgc ggcgctcctc agtctggtgt tcatcgccac cagttgctgc aacattgtct
      481 cctgttgttc tatccgttcg ctcaacggct gccagatgag gacgtaatat ccgacgctaa
      541 acaggaacac caccgctgcc agtaacatgc ccttttcacg cggagaacgc cccgccaggt
      601 gttgtgccag ccagtgttcg ccacggctta actggcgttc acgccattgc tgaaaatagt
      661 gaataaattt atcgcgtaac atgttatttc ctccgcaacg ttacgccgcc ggaaaccgca
      721 tcaccctctt tctgtaatgc gtcctgttgc acaacataat cggttgccag tgcactacgc
      781 agtttgtcga aactggcaaa gttagcagcc cgcaactgga ggtgaagcgt ctggcgtttt
      841 tgatcaaagg tgaaaccacg catttcgatg tcggaaagtg acgctgattt cagggtgctg
      901 gctatcgctg acaactcggc gagcagccgg gtatcgtcgg cctgtgggcg atattttttc
      961 agcgccatcg tcacctgaga gcgtaaattc acaatccgct tctgctccgg gaacaacgtt
     1021 aagaactgtt cctccgcctg ggtgcggctt tgcgccacct gttcgctgac gctccataac
     1081 gtcacgcccc gctccactgc cagcgcaacc agaatcagca atatcggcag aatcatcacc
     1141 cgccagcgcg cccactgttt tcggtagctg acgcgaggct gccacggccc tgtcagcagg
     1201 ttcccttccg gttcgccata agtggtaatg gcgggcagag ctgtaacggt caggcgttca
     1261 gcgtctgcgc cagcccatgc tgatagcttt tccggtgcaa tgccgactac ggttagcgaa
     1321 agcggtaaat tctgctcatt gagctgggca cggaacatga ccggagccag cgcccgcccg
     1381 gcgctccatc cccggcattc atcgatgcgg cagataaccc gttgcgcatc gccagccata
     1441 aacccacaag gaatggacat ccagtccggc gcgacggtag cgcgggtgat gccgttttcc
     1501 tgcaaccact gcgcaatgtt gggcatatgc tgttggtgaa tcactgctac ggttgccagt
     1561 tgctggtcga ttttcaacgg ggcgaaatgc agttcatcga tatcctggtt cagctcttct
     1621 tccagcaagg cgggcagaat agtcggtatc tgcttgcggg cgacatcagg cagttcaacc
     1681 tgccagacgc tgatccattc gccgggaatg tagagtcgaa tcgcatcggt ttgcagccat
     1741 tgctgaagct gttcgtcagc aacgtcaggc cagataccgt gttccacgtc gggtgtacgg
     1801 cgctgccagc ggacggtatc ggaggcgcaa agcgggaaaa aaatctcaag gatggaactc
     1861 actcactttc tcctgtctga tgccagagaa ccgaaaagtg ttgtgggccc atgcggacaa
     1921 ttaacgagtt catcgtcagt tcaatctcat tcacggtgat atctgaacgc agccagaagt
     1981 aattgctgtc cacgctcagg acggttttta gctgtttttt agtacgctca tcgacgtcag
     2041 caagtagcgg ctgtgcaaga aactgatcga catcttccca gcccttcgcc ggacgttgtt
     2101 gtaataacgc ccgcgcctga acagggctta accacgggtc aaacagcgcc tcaagaatca
     2161 cactttgcgt gacgtctaag gtattgatgt tgatttgctg gcgggtcatc ggcagcgcac
     2221 agaccagcgg tttcagtttt tgataaagcc cggcgtccat tccctgtacc acgcgcatct
     2281 cactgatatc agccagcggt tgattcgcgg cgtagaaagg caccgaacgg gcgagatact
     2341 cgctgtcttc acgccccagc cgcgtctgca cgctgcgatc ttcgtcaata aactcccaca
     2401 ggctttcggc tatcagttcg gctcgataag caggcacatc caggcgcgag atcagggcaa
     2461 tcaactgttg taccgcaagc ggacgcgacg ccgttgtcgg ctgagcaagg gcattcaggt
     2521 taaagcaagc ctgtgcgtca cgcagggtga ccgcaatttg cccctgcggc agagggaaga
     2581 aacgagggcc ggatgcccac ggctgtgcca gatgcacgcg cttttcattt ttcagtctca
     2641 gactgagcgc gctcagcgcc aggctttccg cactggcgct gtaccacagc gcctgctggt
     2701 actcctgctg gctgcgcgtt cgcccaagtt gttgctgcat ccgcccggaa agcgtgatgg
     2761 ttaccagcat cataaccgcc agcaatacca gcaccacaac cagtgccatt ccgcgttttg
     2821 gtggtgaggt gatcatgata attgcggccc gcgtaacaac cagatgcgtt caatctcgcc
     2881 ccattgcggc gaatgcagag tcatgcgcac tgccacaggg atcgcctgta ctgacgacca
     2941 gctctcctgc cagcgcgtac catcatagaa ctgcaaacgg agcgaatccg ccggaatcaa
     3001 cttttgcatt gtcggcttca cgctgcctgc ggcatcggtc agcggccagg ctaaccgttc
     3061 gagataaccg tcatgaatgc ggtaaccgac ggtgagcaga ttgctgcgtg gcagacgcat
     3121 caacgggtta accacgccgc cgcgcacaaa gcgcatgcct tcactctcag acgccagcac
     3181 gccagcgcca gccagtaacg ctggttcacg ctggccctga tcgcctctta ccggacgcgg
     3241 catcatttgg gtcagatcgt gggtcagaaa actcatcgtt tgctgcatga ggtttagttt
     3301 ttgatcgtgt ccggcgacgg cgctattcac gcgcgtgacg ccgttagtca cctgctgcgc
     3361 catcagtgcc agcgaggcaa aaatggcaat tgccaccaac atttccagta acgtgaaacc
     3421 agcgcgagtc cttctcactg ttggcctccc acggcgctaa accaggcgcg tcgtgactga
     3481 atcaccgacg aaaagtcttc gtgaaggctg acttcaatat ccaccgcctg gagcagcgcg
     3541 ttgcctgtac tcagcggtgt ggttcgccag taccagcggc ttcctgccat atcgctctcg
     3601 ccctgctgcc tgaccgcccg ctgctcgcgg gtcatcaggt agagcagcgc ggtctggttt
     3661 tcagccaccc agcccgctac cgttttttcc tcaagaattt gctgattact cagcgcaccg
     3721 ctggcaatct gtaacaccgc cgtggcagcc agcgcaaaaa tcgccagcgc gagcatcact
     3781 tccagcaagg taaatccgcg cttcatggca tcctctcatc acattgattg agggataacg
     3841 cgccatcgtg tgccagcttt accgcccaac atgcgttttg cgccgccgag tagaagcgca
     3901 gcgtaaacgg cgtggcgggt tcaaaaggcg aaaaacggat ctgcggcgtc ttctttttcg
     3961 cctctttttg cagctcaagt tcaatatcgt gcagcgtcag tcgacgacgt tgcagctcca
     4021 gcgcatattc cttctgccag atatcacttc cgggttgtaa cagcatctgc acctgtttcg
     4081 gcaccgtaac ctgtgccgat aaccgggtcg aggaaacggg cgaccactgc ccctgacgac
     4141 gctgcataaa ctggtagcca ggcgggtcga ttagcacacc gagtgttttc ccttcgatca
     4201 ctgccctgtc cttaaactgc gcaaagcgag tcagaaaatc ctgcgccgct tttttcgcgg
     4261 gcgattcaga atcggtcgca aaagtctgta ccacgcccgc actggcaagg ccgataagaa
     4321 aaatcaccag catgatttcc agaagtgtga atccgcgttc aggcactgct tactgaaact
     4381 cctgcaaatt ccagttaccg atatctgcgc cagcgccttc cccgttttcc tgaccatccg
     4441 cccccagggt ataaacatca aacagccctt tttcacccgg actgagatac tgataatcat
     4501 tgccccacgg atcctttggc agtcgtttaa tgtatccacc agtacggtag ttgcgggagt
     4561 ccgccatatt ggctggctgc tggatcagcg cctcaagccc ctgttctgtg gtcggataac
     4621 gcccgttatc cagtcggtac atatccagtg cgttttccag cgccacgata tcgctgatgg
     4681 ctttttgccg atcggctttc tctttgttgc ccaacaggtt aggcaccacc agacttgcca
     4741 ggacgccaag aataacaatc accaccatca cttccagcag ggtaaaacct gcccgtggtt
     4801 tttgtgtgcg ggataacgaa ttcatgtgat aactccgtaa attacattcc aaccatatta
     4861 ttcagttgca gcatcggctc gaggatggcg atgacaataa acagcaccac gcccgccatc
     4921 atcaccacca gcgccggttc aaacagccct aacgccagcc ccacctgggt atcaaattcc
     4981 cgttcctggt tgactgcggc ctgctcaagc atggtttcca gttcgccgct ctgttcgccg
     5041 gaggcgatca tgtacagcat catcggcggg aacaggcgca actccgccag cgcggcgcgc
     5101 agactgcttc cttcgcggac gcgatctgcc gccagcagta gttgttgttc gacatagcga
     5161 tttgccgaca cggcggcagc agtctgaata ccttccagta gcggaacact gctggcggtg
     5221 aggatgctta aggtgcggga aaaacgcgcc gtattcagcc cgcgcgcaac acgccccgtc
     5281 acaggcaggc gcagcacggt ttgatcccag cgcaggcgca tagcaggatt tttaagtagc
     5341 cgttgcccca gcaccagaag ccccagcaaa cccgccagcc agtaaacgcc gctggcctgt
     5401 aacgcgtcgc tcatggcaat aagcgtgcgg gttgaggcgg gtagcgcgtg tccgagatga
     5461 tcaaactgtt cgatgatttt cggcaccact gccgtcagca aaatagtgac tacgcccgtt
     5521 gccaccacca gcagaaccag cggatagagc atggcctgca atagacgtga tttcaggcgc
     5581 tgtcgctgtt cggtgtaatc tgccaggcga ttaagcacca cgtcgagatg cccggatttt
     5641 tctccggcag caaccatcga acaaaacaga gaatcaaaga cgcggggatg ttcgcgcagg
     5701 ctgtccgaca gggtgtaacc ttcctgaatc cggctgcgca gcgccattcc gaggcttttt
     5761 acatgcagtt tttcactttg ctcactgacc gcctgtaagc aggtttccag cggcattgct
     5821 gcctgtacca gcgttgccag ttggcgcgtg aacagcgcaa gatctgccgc cgccacgcga
     5881 cgatgtgcgt gccgccgacg ctgcaacatc ccccctgacg aagtattcat ccgggcttca
     5941 atatgcacgg ggataagctc tttaccgcgc aacaactggc gggcatgacg cgcggaatcc
     6001 gcctcaatca tacctttggt tttgcgacca ttacgctcca gcgcctgata gtaaaacagt
     6061 gccattacgc ctccatggtt acccgcagaa cttcatcgag agaggtttct ccggcgagca
     6121 ctttctcaat gccgttgctg cggatacccg cagagtgttg tcggacataa cgttccagct
     6181 ccagctcccc ggcctgacgg tggatcaaat cacgcaatgt ggcatccacc acgatcagct
     6241 catggatggc agtccgtccg cgaaaacctt tgtgattaca ggcgggacag ccctgtggat
     6301 ggtacagagt gacggtacgg gcgtcggtaa ttcccagcag gcgtttttct tcgtcagtgg
     6361 ctggtgcggg ctggcggcag tcgggacaca gcgtgcgaac cagtcgctgc gccatcacgc
     6421 ccgtcagact ggaagagagc aggaaaggct ccacgcccat atcctgcaaa cgtgtgatcg
     6481 cccccaccgc tgtgttggta tgcagcgtgg aaaggaccag gtgtccggta agtgaagcct
     6541 ggacggcgat ttctgcggtt tcggtatcgc ggatttcacc gaccatcacc acatccgggt
     6601 cctgacgcaa aatcgcgcgc agcccacggg caaaggtcat gccgacgcgg gtgttaacct
     6661 gcgtctgacc gatcccttca atcatgtatt cgatgggatc ttcaaccgtg agaatgttgc
     6721 gcgaatggtt gttcagctcc tgcaatccgg cgtacagcgt ggtgcttttg ccggaacccg
     6781 tcggccccgt caccagaaag atgccgtgcg atttgtgtaa cagctggcgc aactgcgcgg
     6841 tcagttgctg gctaagcccc agacgctcca gcgtcaggcg ggcctggttt ttgtccagca
     6901 gtcgcagcac cacgcgctcg ccccaggcag aaggcatggt ggagacacgc acgtcaatcg
     6961 cccggccgcc cagcaacagc gcaatacgtc catcctgcgg cacgcgcttt tcggcaatgt
     7021 ccagccgcgc catcaccttg atacgcgaca ccagcagcga ggccagtttg cgccccggac
     7081 gcagcatttc atgtaatgtg ccgtcaacac gaaaacggat caccagactc ttttcaaacg
     7141 tctcaatgtg gatatccgaa gcgccttctt tgattgcctc tgccagcatg gcgttgatca
     7201 gtttgatgat cggcgcatcg tcgtcgcttt ccagcagatc ttccgtttcc ggcagttctt
     7261 ccgcgagggt aaaaaagtcc tcggcagaac cgagatcttc catcagttgg cgagcctcgg
     7321 aagagtcccg ctgccagacc gcattcagcc gctgttcaaa ttcggcttcg tcgatttgcc
     7381 gcagcgtaaa gggcgcgttc agcccccgct gcagctcctg caaaacagag agcgatagcg
     7441 gatgaacgtg gaggatctcc agcgacgctt cgcaccatgc caccaggcta aaccgacggc
     7501 tgaaactgta gggcagacgc acggtgttgg cggtggtttc ctgtgctaca ggcaccatta
     7561 acgcgttctc ccggcattga ggaacgcgcg aacttccggc ggtaaggcct ggttttgcgc
     7621 tggcagtacc ggctgtgcgg tgtgcggcat caggcttaaa ccttgctcat cacgatagat
     7681 ctgttcggcg cgcatatagt tatatttgcg ctgcgacacg ccgtctgccg ccataccgtc
     7741 acgcagaatg gtcgggcgga taaacaccat caggttacgt ttttcttttt tatccgccgt
     7801 cgatttaaac aggttaccaa tcaatgggat atcgcccagc agcggcactt tcgccacgct
     7861 ttctcccgcc tgatcgtcca tcagaccacc aagcacaatc agctcaccat cgttagccag
     7921 cacggtggtt ttcagtttgc gctcaccaaa caccacgtcg aggctggtct gtccttccac
     7981 cttcgagact tcctgctcaa tcaccatctg taccgcgtta ccttcgttaa tctgcggcgt
     8041 gactttcagc atgatgccga cttttttcct ttctactgta ttgaaaggat tgctgttatt
     8101 ggagccaacg gtagagccgg ttaataccgg aacgtcctgg cccaccatga agaaggcttc
     8161 ctggttgtcc agcgtggtga tgctcggcgt ggagagcacg ttcgagctgg agtcgttttt
     8221 aaccgcctgc accagtgcca tccagtcgcc tttcaccacg ccaaccgccg taccgctgaa
     8281 tccagaaaga agctgggcga gtgtggagag atcgccgttg gtatccggat taatggtggt
     8341 agcgccgttt tcactgatca ccgtggagcc tttctgcggt ttcgcctgag aaatggctgc
     8401 ccccagcgtg ccaataggga tctgcgtacc gttagcaaac tgcattaatc cggcatcttt
     8461 cgacgcccac tgcacgccga aattgatatt gctaccttcg gcaacttcca cgatcaacgc
     8521 ctcgacgtgc acctgagcac ggcgaatatc cagttgttca atcacgcttt gcagcgactg
     8581 catgatgtcc tgcggcgcag taacaatcag ggcattactg tgtttgctgg cggcgatgga
     8641 gacaacctca cgcccgctac cgaccgtgcc ttccgcctct tctttagccg ccgtgagcgt
     8701 gccgctgacc tgcttcagca catcgaccag atcttcggct ttgctgtatt tgagatagaa
     8761 aacctggctg ttgccgctgc gttccatttc tgagtccagc cgacggatca ggcggcgcat
     8821 tttgtcccgc gtggccgggt caccactgac aatcacactg ttggtgcgtt cgtcggcgac
     8881 aatttgagat ttcagcgtcg caggctggtt ctcgccgctg tttttcgtca ggctttccag
     8941 cacgcgggcg atttccgaag ccgaggcgtt atccagcggg atcacctctt cggtgcgatt
     9001 acccgcgtga tccacgcgct ggatcacttc tgtcaggcgc tccacaacgg aggcgcgtcc
     9061 ggtgagcata atcacgttgg agggatcgta attaacaaca ttgcctgagc ctgcgctgtc
     9121 gatcatctgg cgcagaatcg gtgccagctc gcgtaccgac acattacgta ccggcactac
     9181 tttggtgacc atttcatcgc ccgcgtagtt gtcgctgcct tcaccgacca gcggcagcgg
     9241 ctcgactttc gcagcgctgg atttcaccac cttcagcacg tcgttttcca tcggtacgac
     9301 ggcatacccc tgtgcttcca gcaggttaag gaatagctgg taatactggc gttcattgag
     9361 cggggtcatg gtgcgaatac tcactttccc ctgtacgccc ggccccataa tgatggtttt
     9421 attaaggtta gcgccgacgg tttcgatgaa cgatttcagg tcggtatctt taaaattagc
     9481 ggtgaaagtg gcttcttccg cccagaccgg tgaactacat aatgccgctg ccagcaccag
     9541 cggcagtaaa cgcttttttg ttttgaggcc agttgtcttc ttacgccaga ccgacaacgt
     9601 aatatcacgc caaaacacga tgaatgattc tcctggatta aatgcggtta gcgcagcgcg
     9661 atggaaatgt cgtggcgcgc acccttgcgt aaaaccgtaa gttgaatgga atccattgaa
     9721 ggtaactgcc gcatcagagc aatcattgct cgtggatcag tgaaatcctg ctgatttagc
     9781 gcaatggcga tatcgccttc cttgaaaccg ctggcatcga acagagaacg atctgccccc
     9841 ggcttcactg cataaccgac aatcccctcc ttacgcacag gcgtaagctg gatatagtta
     9901 aaaattttct gcggatcttt cgccagtgcc tgacgcacgg cagtcgggat ctcaactggc
     9961 gcactggcag caggctcagc aacagcttgc tttgcttcgt cactgacagc ttttttgttg
    10021 gtcacggcaa cggtggaacg ctcctcttca gccagactca ggcgctctat ttttccctga
    10081 tagcgcagca tcacatggtc gcgattgatt tcctcaatca ctgcgttgtg cgagccaagc
    10141 gtttcaccct gcaaatagac ctgctgttta ccgccttctt caataaccgc gccgggtctg
    10201 gcaccaaagg caatcccgcg cagcaccaca ttaagacgcg tttctgccac aggcacaggt
    10261 tcgggttgtt ttaccggcgt ggcgacgggc tgatatttgc caaaccagtt ttgctggctg
    10321 attaattgca cgtcattttt atcgaacgtt tttgcatccg cacggggcgg tttattcgcc
    10381 gatggggaaa cagccgtata ttccgcagaa aaggagaaat agcgccagag tgaatgcgcc
    10441 acttttgcag aaataataag cagcatcagc cagaacatcc cgcgtacaat ttttcgcaga
    10501 tgctctttgt ctgtattaaa agattgtctc tggttaagag tgtgacgaat ttttgtcagc
    10561 cattgaatga gataaattcg tgcgtcacga aaaacaaccc gcgccaattt atatctaccc
    10621 gacgttatgc tttgactatt ccacaggtgg tacgatccag tttccgctga tactggttac
    10681 ctgtacgtgt atcattaatc gttatgctgt aattaattcc ctcggtaatc attaatttca
    10741 ccgttgggtc agcgcacatt tgtcgttgat agctggttaa aaaggcgtca ggcgtctgcg
    10801 tggtctgagt ccccgcttcg ctgataatgg tcatttttac cgtcgtgccg ctactttgtg
    10861 ccagcactaa ggtatatccc gcagatttaa tcggcaggtt ttggctgata ttttgcgcct
    10921 gttttttcgc cagtaaactg gcattttcat tatggctggc acagccactt aataatcctg
    10981 taacgctcaa caatagcgat attaataccc tccctggcat ttgttttatc gacatggtaa
    11041 ataatctcta aggttattaa taagagttaa aatgtcactt tgataatgac gttgttatca
    11101 ttaaaacaat gcctgtagat aaattgttgc tataccgccc agacttaaac acggtccaaa
    11161 aggcagtgtg gttgatcctc tttttgtaat aacggcatat atcaggccgc agcatgatgc
    11221 gattaaagca acattgggta gcgacaacgg ccctacccaa ctacctaacg cagcgaacaa
    11281 taatacatcg cccatgccta atgcttcttt acgcagaact attccggcta tccagcgcag
    11341 agagtaaaaa gtgataaacc ccaccaggac gccggtgact gcatcttgta gcgtgagcgg
    11401 gctctgctgc gcccatgccg caattagtcc cgtccacaat acgccctgag taaaaacatc
    11461 gggcagccat tggtgatcga ggtcgatgat gctcgcggca atcagccagg cggataatat
    11521 catcaccgcc agcgcccatc cactttccgg ccagaccaga ctcgccagca aaaaagcgag
    11581 tgccgtcaat aactccacca gcggataacg cttgctgatt ttcgcctgac agtcgcggca
    11641 gcgccctttg agcatcaacc aggagagcag cggaatattg tcacgaacgc ggatagtctg
    11701 ctgacaatgt gggcagtgcg aacgcggtag cgcaaggctt atttttgact gcgcactcgg
    11761 catttcacca tgaaactccg ccatttgttg gcgcagcatg atggggtaac gccaaatcac
    11821 cacattcaaa aaactgccga tgatcaatcc tccgacggta gccagtatgg gcatcgccac
    11881 ggggtattgc tgaaaaacat caaaaagcat ggttaaaggt tgtttgttgt aacttgccgg
    11941 atgcgggcct gcaggtgtat gccatatggc tttcctccag gcccgataag ctggcgcaac
    12001 ccggtgcgcc ttatttcatg ccggatgcgg cgtgaacgcc ttatccgggt tattcagcag
    12061 gcatcgaata ctcggtaacc ttgttaatgg tttccggccc ttgcttcggt ttcggcagat
    12121 tgagtgacgc cagcgtgttg tacgccgact ggctcacacc gccttcgaag ctcatctccg
    12181 tcgctcccgg caactggtaa gcattcgcgc ccggattcca tttcttaaag aactccgaaa
    12241 gatccgtctg ggcgacccag gaggcacaca gcatcagcgt gtccgctgcg ttaccgttgg
    12301 attcagcaca gtaattcttg ccgccaaact tgtcattgct gacctcatcg ccgcgtgctt
    12361 tacgatgcat caactggaac aggttccagc ctttcatccc ttcacgctcg ctgtaaaact
    12421 ctggcagagg agtgccatct ggataccatt tcttgatatc aaagtttttc tctgcccatt
    12481 ccttcagctg tgcgtacatc agcagacggt cacccgcacc gccgcgcgcc catgcctgac
    12541 cgttgctttc ctccagatat tccggtgcga cggtaatatc gtcagcgaca cggttcatct
    12601 tgccgagata acgatcctgc atgtacagcg ccagcacgtt gttagcgact tcagttgcac
    12661 ccggtacagt caacggcgtt tctgcggcgt tatgaccgac ttcatgccag atcagccagt
    12721 cgttcagcgg cgtcgtcggc agcgtggtgc tgttcggcga gaagctgctg ttcattaccg
    12781 gataacccga atgcgcatca ccgatggaga tctgcacatc gttggcgaaa cgatgtttgt
    12841 ggcccggcaa gtttttatag gtaaacatcc ggtgcttacc gtcttcgcta tcacggccgt
    12901 agaagtcatt catcgagctg gcaaaggtat ccagatcgtt agcgaattgc tccagtccgc
    12961 cagtgtaatt gctggcattc aggttcttct tcggtgtggt atagacgaac gaggcagact
    13021 ccagttcgcc cagcggggca ggggagttca gatcgtgttg ccacttaccg tctttataga
    13081 acggcgcttt tactacaccg gtaaaggtga agtcagctga ttgcacctct ttgctgtcgc
    13141 ccttgatgta aatcagacca ccgtaaggga ctttgaacgt caccttatca ttggctttca
    13201 ggtcataggt ttttgtcact ctgggtggac gattcaggct aacttcatgc ttctctcgtc
    13261 ctgtcaggtc gtcggccagc gcaacagtca cggtcacagg aactgtcgcc tttgactcaa
    13321 tgctgacttc ctgctgggca ggtgcccaca ggccagttga ctgcatgtta cctgcaaacc
    13381 atttggttgg agctgagtac aagttaatgt tttgtgtgac ggtttcgccg tttgtattca
    13441 ccactcccgg atacttctca acatcaactt tgatgttcag atcccaccag gaacggccca
    13501 gcatcaggcg tgtcagcggt ttttccatat agttgagcgg gtagctcggg ttcatcatgc
    13561 ccgctttgct gctttcttca ccataaatca tcttgttatc gatcagcgat tttttcagct
    13621 cagcagaaca ctgcgtgcca acgtatgcat tgttggtgta gcagttcaga aactcagtga
    13681 acgttttaaa gcccagctcg tcttctttac cctcttcata acgatatttc gtctcgttcc
    13741 acagccagac ggacatgttt tggtataaac gttccagatc aacgctgttg agacgctcac
    13801 cctggcggcc attggtacgg aaataaagct catgctgata caggcgctga atattggtgc
    13861 ctaaatccgc agcctgcagc atcgctttcg cggtgtcggc actcagatcc agctgcgtat
    13921 actgcggaac atacatgcca ccggttaccg gcacatccgt gcctgggcgg tattccagac
    13981 agttgacctc atagtggtag tcagaatcct tacacacttc cagccctggg aacgcgtcca
    14041 gaattctctg ttttgccgca gccagtgact caggcgtttt gtggtcggct tcatcaataa
    14101 aggcgaattg ggttacctgt ttaccctcaa cttcttcctg ccagctggca acttccagct
    14161 tcggtttgtc atcaggtttg ttttcttgct gatatttcca gataacttct ttcgtggtgt
    14221 catcaatggt atacggtggt ttaccatcga cagccggata acgctcatag acccaaattg
    14281 gcgttgaacg tcgttgacga acgcggtccg ggtagccttg cgggtcgtta tttactaccg
    14341 acttgttaag cgccatcgac aaacctgcgg catcaagcag acgtacaaag ccagatgcgc
    14401 tctcttcctt aagattgctc atcacgtttt ccatgatcag caccgatcca cctttgttca
    14461 tataggcgat caaatcggtc acatcctgct gggtcagctt cggtttgctg gtatctgcgc
    14521 gcagaggaat ggagtaagga tcgctacccc attgtgtgac gtattcaaag ccattgagga
    14581 tcaacagagg aacttcatct ggattcagat tgccatagct ggtcatcggt ttgaccgtga
    14641 tgccagtgaa atccttgtgg aacgcaaacg gtgcgctatt tcccagcacc tgcccatgtt
    14701 ttttgaaata caccgtctcc aggttagtac ccacggtcat actggatttt gcatccggca
    14761 accagcgatc atttgacaga tagcgcagca cgttctccat gaagttcttc atgtcatccg
    14821 ggtcgctgtt gagcgtacac tgcccatctt tattaacgcc cccgttccag ctgtaaccgt
    14881 tcgggcaacg caggatgctg ttgtagtgtg ggttaccgat aaccatcagc ttgccatcgc
    14941 ccacttgccc cagcgagata aacggcaggt tgaaggtggc ggtttcgcgt gtcacgttct
    15001 ctggtcgcac aatggaaggc gcttcagtaa tgtacgccag ctcattttta tcccaggcgc
    15061 gtttttcgcc aaacgccagc cagtagtttt tatcattacg cgccatcaga atcgggaagg
    15121 ccgcgttgga gatattcacc accgcctgac cgcgcgcatt acccgtgctg ccatagaagt
    15181 tggtagagtc atggaataca tggaacttac tgacagattt gtagttcgta tccacgcccc
    15241 acagcttgtt gataacgccc tgaatctggc cgtcattaac attgcgcgtt gtcagcgaga
    15301 accagcgagc ctcgttacaa ccatcggttt tcgcacaaat cgcggtatcg atctctttgg
    15361 cctgacccgt cttaaactgc tcgataaatt cgttaggcag attaacgact tgctcacctt
    15421 cccccagcgt cgcaccattg gataacgaga gattgataat ctcgttaatc acgttgggat
    15481 attcagcaaa aaccttgcgc acttcatccg gaactacacg cgtgtgattt tgtccagcct
    15541 tcgaatagcg gtgaatcaac tgatcgatat ttgccccgcg aacttcatca cccagttcag
    15601 tcaatgcaat agtcgacttg ttaccacgca cagaaccaag ctcaaaagtg tcgatgccaa
    15661 aggagatggt ttccccccaa ctaaaggaaa attccccgtt ttctccggtt acaccacggc
    15721 cggaattggt gtaatagttg acaccaacaa caccatcccc ctgactatcg accagtcgac
    15781 cttcagagag aatgatttca gtgggttgat actgataaaa ctgttccgcg ttagccgaca
    15841 cgaaggaagc gtttagatcc ggttttgttc ccggagtggt ggcgggcacg accggtgaag
    15901 tatgagtgga tggcgcttta tcggtcgcgg cattgttttc cacctcttca ttgaccagct
    15961 ttttgaattc ttccggtgcc agatcgattt gcttatacag cgagtcgaaa cgtttactct
    16021 cgatcaccga ggagaactcc aggcacacct gttctgtatt cgccgggcaa ctgttcatgg
    16081 aggtgaccag cgagagcgca ttgcttttct tgttgtcgga acccgccagt tcttgcgcgt
    16141 cctcaagact aaacgacact ttttcaaccg cacgcaggct acgcgcagct tctgactggg
    16201 tgtcgaaggt agcaattgtc gtgttgttcc ctgccacaca ggtgactttg tcgcctggtg
    16261 taaaggtaaa gccatcgctg gattcatcat tacaggtgat atcaccagtt acccgcaggc
    16321 ttccgcccag agtcagataa cccgttttcg taggaacagg ttctggctcc ggttctggtg
    16381 ttggctcagg atcaggtgtc ggttccggcg taggttctgg gtccggcgtt ggctcaggtg
    16441 tcggctccgg gtttggtgtt ggatcaggtt tcacttccgg caaagaccct gttccagaat
    16501 ctacaggcgg cgtatcggag gaagatccgg agccaccgcc atcacaaccg gctaacaggg
    16561 ttgcactcaa aatagccgct aaaagcgatt tcttatattt aaatttctta ttcattaata
    16621 acgcaagtga caaaacaatg
//
LOCUS       DAZFFI010000095        16615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFFI010000095 DAZFFI010000000
VERSION     DAZFFI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.095"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(151..957)
                     /gene="trpA"
                     /locus_tag="ACHJ12_003693"
     CDS             complement(151..957)
                     /gene="trpA"
                     /locus_tag="ACHJ12_003693"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4991420.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(957..2150)
                     /gene="trpB"
                     /locus_tag="ACHJ12_003694"
     CDS             complement(957..2150)
                     /gene="trpB"
                     /locus_tag="ACHJ12_003694"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4991421.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2162..3523)
                     /gene="trpCF"
                     /locus_tag="ACHJ12_003695"
     CDS             complement(2162..3523)
                     /gene="trpCF"
                     /locus_tag="ACHJ12_003695"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4991422.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3524..5119)
                     /gene="trpD"
                     /locus_tag="ACHJ12_003696"
     CDS             complement(3524..5119)
                     /gene="trpD"
                     /locus_tag="ACHJ12_003696"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4991423.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5119..6681)
                     /gene="trpE"
                     /locus_tag="ACHJ12_003697"
     CDS             complement(5119..6681)
                     /gene="trpE"
                     /locus_tag="ACHJ12_003697"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4991424.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6703..6796)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6773..6817)
                     /gene="trpL"
                     /locus_tag="ACHJ12_003698"
     CDS             complement(6773..6817)
                     /gene="trpL"
                     /locus_tag="ACHJ12_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4991425.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6955..7836
                     /gene="rnm"
                     /locus_tag="ACHJ12_003699"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6955..7836
                     /gene="rnm"
                     /locus_tag="ACHJ12_003699"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4991426.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7833..8453
                     /gene="yciO"
                     /locus_tag="ACHJ12_003700"
     CDS             7833..8453
                     /gene="yciO"
                     /locus_tag="ACHJ12_003700"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4991427.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8554..9426
                     /gene="rluB"
                     /locus_tag="ACHJ12_003701"
     CDS             8554..9426
                     /gene="rluB"
                     /locus_tag="ACHJ12_003701"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4991428.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9466..10056)
                     /gene="cobO"
                     /locus_tag="ACHJ12_003702"
     CDS             complement(9466..10056)
                     /gene="cobO"
                     /locus_tag="ACHJ12_003702"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4991429.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10053..10811)
                     /gene="yciK"
                     /locus_tag="ACHJ12_003703"
     CDS             complement(10053..10811)
                     /gene="yciK"
                     /locus_tag="ACHJ12_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4991430.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11031..12080
                     /gene="sohB"
                     /locus_tag="ACHJ12_003704"
     CDS             11031..12080
                     /gene="sohB"
                     /locus_tag="ACHJ12_003704"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4991431.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12116..12367)
                     /gene="yciN"
                     /locus_tag="ACHJ12_003705"
     CDS             complement(12116..12367)
                     /gene="yciN"
                     /locus_tag="ACHJ12_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4991432.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12747..15344
                     /gene="topA"
                     /locus_tag="ACHJ12_003706"
     CDS             12747..15344
                     /gene="topA"
                     /locus_tag="ACHJ12_003706"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4991433.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15554..16528
                     /gene="cysB"
                     /locus_tag="ACHJ12_003707"
     CDS             15554..16528
                     /gene="cysB"
                     /locus_tag="ACHJ12_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4991434.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4054 a         4206 c         4414 g         3941 t
ORIGIN      
        1 tatattactg ttgggcggaa aaatgacgta agttgacgtt cgaccggggt aagcgaaacg
       61 gtaaaaagat aaatattaaa tgaatttagg atttttccgg cttcattaaa gaaagttaaa
      121 atgccgccag cggaactggc ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg
      181 tacaaaaact ttcagtgccg ccagcatttt ctctggctca ttaatatgtt gctcgatgat
      241 tttaacaatg gccgaaccag aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac
      301 ctgatccggg gcggaaatac caaacccctg caatggaggt gcagcgttgt actctttcag
      361 cttcgcaacc agatgattga ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc
      421 tcgtgacagc aaataggtgt aaccacgacc gtaagaggct atctggcgca gcaggtcgtc
      481 atcggcattt ggcgggcaga tgaagatagg tgcgacatta tggcgcaacg cggcctggcg
      541 gaagggcgcg gactcttcaa ttggcacatc ggcaaccagc accgaatcga cgccgacttt
      601 ctcgcactgg gcataaaact catcaatgcc tttgttaaac accagattgg catacatcaa
      661 caggccaatg ggaatggtcg ggtgtttctg gcgaatcagt gccagcattt caaaacattg
      721 tgccggagtc acacctgccg caaaggcgcg cagagtggcg ttttgaatcg tcgggccatc
      781 cgccagtggg tcggagaagg ggatacctaa ctccagcgcg tcagcaccgg cttcaattag
      841 cgtatcgata attttcaatg actgctcaat gcccggatca ccgagcgtga cgaaaggaac
      901 gaatgcgcct tctttgcgct ccttcaactg ggcaaacaga gattcgtagc gttccatcag
      961 atttcccctc gtgctttcaa aatatcgtga acggtgaaga tgtctttatc gccgcgaccg
     1021 gaaaggttaa ccaccagtag ctgctcttta tccgggtttt cgcgcatcat tttcaacgca
     1081 tgggccaggg cgtgggagga ttccagcgcc gggatgatcc cttcgtgcag gcacagcgtt
     1141 ttgaaggctt caagggcttc atcatcggta atagacacgt aatcagcgcg tccagtgctg
     1201 ttaagatacg cgtgttgtgg gccgacagac gggaaatcca gtccggcgga gatggagtaa
     1261 gattcttcaa tctgcccgtc ttcggtttgc atcatcggcg ctttcatacc gaaatagatg
     1321 cccacgcgac catgttttaa cggtgcgccg tgctcgccag tttcgatacc gtgaccacct
     1381 ggctccacac caatcaggcc gacgttggtt tcattgatga aatcagcaaa catgccgatg
     1441 gcattcgaac cgccgccaac acaggcgata acggcatccg gcaggcgacc ttctctttcc
     1501 agaatctgcg ctttggtttc ttcgccaatc atccgctgaa actcacgcac aatggtcgga
     1561 taaggatgcg ggccagctgc ggtgcccagc atatagtgcg cggtttcgta actaccggac
     1621 cagtcgcgca gcgcctcgtt acaggcatct ttcagcgtcg cggaaccgct atgcaccggg
     1681 atcacttccg cacccattaa gcgcatacga aaaacgttag gcgactggcg ttctacgtct
     1741 ttggcaccca tataaatacg gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc
     1801 acgccatgct gaccggcacc ggtttcggcg atgatttcgg ttttacccat ccgcttcgcc
     1861 agtaaagcct gaccgagcac ctggttagtt ttatgcgcgc cgccgtgcag caaatcttca
     1921 cgcttgagat acagagtggt gttcgtcccg gctgtaatgt tctggcattt ggtcagcgcg
     1981 gttggacgcc cggcatagtt tttcagcagg tcgttgaact gagcctgaaa ttcaggatct
     2041 ttttgcgcac tgacaaaagc ttcttccagc tggcgcagag caggcatcag gatttgtggc
     2101 acgtacatgc cgccaaactc accaaaatag gggttaagta atgttgtcat tgttcctttc
     2161 cttaatatgc gcgcagcgtc tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg
     2221 gttgcgactc tacagcagaa ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca
     2281 cgcagttatc tgcgcctaag ccccccgcca gcagaacgtt gccaagcgat tgaccattta
     2341 atagtgacca gtcgaaacgt tgcccgcttc caccctggcc gttgtctaaa acatatttat
     2401 caacgtgctg aagctcgcgg gcgggcaggg tttcaccgac gcttaatgct ttccagatgg
     2461 caacgtgtgc tggcagagct tcacgcagcg tatcgatata cagctgatct tcattaccat
     2521 gcagttgcac tgccgccagc gataacacct tagctttgtc caccacatcg gcaatatcgt
     2581 gattgcggaa cacgccaaca tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt
     2641 caacgttgac gcaacgcggt gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg
     2701 cgtcataagc tgctttagca tcttgcccac gcgtcaggcc acatacttta ttctcaccca
     2761 gcaacacccg gcgcacggcg gcgtgcaaat cgtcatgggc catcaacgcc gaaccaatca
     2821 gaaaaccgtt agcgaagtgg cttaactcgc gcacctgagc gtaagtattg atgccggatt
     2881 cgctgattac cgttacgttg tgccccagtt tcggcgcaag ctcgcgggta cggttgagat
     2941 caatcgacaa atcccgcaga tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa
     3001 tggcacgctc cagttcctct tcattactga cttcggtcag cacacccatc tccagactgt
     3061 gggcgacggc ggcaagctgg cgatattgtt cgtcatccag tactgaaagc attaataagc
     3121 aggcatcagc ctggtaatag cgcgccagat agatctggta aggatcgata atgaagtctt
     3181 tacataaaat cggctgcggg gcgatttggc tgacgatggg gaggaaatca aagctcccct
     3241 gaaaatattt ctcatcagtc agcaccgaaa ttgccgaagc gtaatgttta taaatggcgg
     3301 caatgcgtgc tggatcgaaa tcatcacgga tcacgccttt tgacggcgac gctttcttgc
     3361 actccagaat aaacgccgtg cgcgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg
     3421 gctgaacctc attctgaaaa ctggccagcg gttgctgctg tttgcgggtt tctacccaaa
     3481 tcgccttgtc tgcgacgatt ttcgctaaaa cggtttgcat catttaccct cgtgccgcca
     3541 gcgcggtaac tctgtcgtaa gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat
     3601 tggcttgcag atcttcatgg ccatgcaggc gcattaacat ggcgacgttc gcagcgacgg
     3661 ctgcttcatg ggcggcgtcg cctttacctt gtaacaaacg tgttaaaatg tcacggtttt
     3721 cttccggtgt tccgcctgcc agttgctcct ggtggtaggg tgtcaggcca aaatcttcag
     3781 cggtcaattg atagctctta atttcgccgt catgcagctc ggcaacgatt gtcggcgcgt
     3841 gtaatgaaac ttcatccatc ccgccgctgt gcaccaccgc cgcgcgttga taccccagca
     3901 cgcgcaaggt ttcggcaatc ggcagcacca gttccggact ataaacacca attaacgcca
     3961 gcggcggatg cgccgggtta atcaatggcc ccagcacatt gaacagggtg cgggttttca
     4021 gttgctggcg aaccggcatc gcgtggcgga atccggtgtg atacttcggc gcaaagagga
     4081 aacatacacc taactcatcc agcgcctggc gcgatttatc ggcgttcata tcaagattaa
     4141 taccgaacgc cgccagcaga tcggacgaac cagatttact ggagacgcta cggttgccgt
     4201 gtttcgccac tttcagccca caggccgcgg cgacaaacgc actggcggta gaaatattga
     4261 tactgttgct gccgtcaccg ccagtaccga cgatatcagc aaacagataa tccgggcgcg
     4321 ggaacggcgc ggcgttttcc agtagcgcgg ttgctgctcc ggcgatctcg ttcgggtgct
     4381 caccgcgaat tttcatgctc accagcgccg ccgccagttg ttccggcttc agctcgccac
     4441 gcaccaccgc tgaaaacagc tggtggcttt cttgttggct aagcgtctgc gcctgataca
     4501 gtttttccag aatcggttgc agcgtgttgg ctggctctag tttctgctgc gcccaggcca
     4561 gcgtttgttc cagcaggcga gcgccctggg tggtgagaat ggattccgga tggaactgga
     4621 atccacaaac gcgatccgca tcgtgacgta ctgccatcac catgccatta aaatgggcgt
     4681 tgatggttaa accggccgga atgttactgc caaccagcga gtgataacgc gccaccggca
     4741 gcgggtttgt taatccggca aacatcgcct gaccgtcatg ttcaatgctc gacgctttac
     4801 cgtgaagaat ttcgcccgcc tgaccgacat agcccccgta agcttcgaca attgcctgat
     4861 gtccgaggca aatgccaata atgggcagct tgccacgcaa gcgggtgagg agttccggca
     4921 tacaaccggc ttcgctcggc acaccggggc caggagaaag catcagtacc ggattgctca
     4981 tcgtcgccag gcgttcaatt aaggtctgcg ccggaatatg gttgcggtaa atcaccacat
     5041 tatgcccatt gctgcgcaac tgatctgcca ggttgtacgt aaaagagtcg atattatcga
     5101 gcagcagaat gtcagccatc agaaagtctc ctgtgcatga tgcgcggtgg caatagcgcg
     5161 cagtacagcg cgggctttat tacgggtttc gtcggcttcc gactgcggaa cagaatcaag
     5221 gactacgcca gcaccggctt gcacggtggc gataccgttt tccaccagcg ccgagcggat
     5281 cacaatgcag gtgtcgagat cgccatgcgc ggtgaaataa cctaccgcgc cgccgtagct
     5341 gccgcggcgg cgaccttccg cctcggcaat taactgcata gcgcgtactt tcggcgcacc
     5401 acttaacgtt cccatattca tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg
     5461 cagttcaccg accacgcggg agaccaggtg catcacgtaa gagtaacggt caactttggt
     5521 gaggtcggcg acgtagcggc tgccgggggt gcaaatgcgt gccagatcat tacgggcgag
     5581 atccaccagc atcagatgtt cagaaagctc tttatgatcg gtacgcattt ccagttcgat
     5641 gcggctgtcg aggtctctgt ccagcgagcc atcggcacga cgaccgcgtg gacgtgttcc
     5701 ggcaatcggg tagatctcaa tctggcggct ggtggcgtca tacttgagcg aactttccgg
     5761 cgacgcgcca aacagggtga aatcattatc ctgcataaaa aacatgtacg ggctgggatt
     5821 actctttttc agcacgtaat aggccgccag cggtgacggg cagggcagag agaaacggcg
     5881 agatggcacc acctggaaaa tttctccagc gcgaatcgct ttttgcaaca aacgcactac
     5941 gccaccgaac tcttcatcgc tctgattaca ttcacaacgc atatgcggca cggaaaccac
     6001 tggcagcggc ggcgcggctt cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag
     6061 acgttgtttt tcttcttcat tcggagcaaa caggctggcc tgaatgcggg tgcttttttt
     6121 ctgatggtca atcaccatca gagtttcagc gagataaaaa cagaaatcag ggcagttatt
     6181 ttccgctgac agttgcggta aatcttcaaa tcccgccaca aggtcataag agaacaggcc
     6241 gccgaagaac attgcttctc gttcttcctt cggtacattc aacagattct gcaataaacg
     6301 gaaagcgtca aaaaccgaaa gggagcataa gcgagcgtct tcatccagca gtggactgac
     6361 aggggggaag cgcagcacac ggcagtttgg taattgttca ttttccacac ccgcaggcag
     6421 ggcgttatcc agtagtgtca gcagggcttc gccgttgccg gaaagtgcct ggattgtgac
     6481 agtgtcacct aaagctgtaa tgcgcagcgc actgtctacc agcagcaggc tttttaaatc
     6541 atctttgctg tcgatatctg cggattccag cagcagcgtt gccggacgat ccccacacaa
     6601 ctggtgaaaa agcgcggtgg gattgtcgcg ataagcgcct tcgcaggtta gcagttcgag
     6661 agtcggtttt tgtgtttgca ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat
     6721 taggcgggct gggtatctga ttgctttacg catggtgaat acactgcccg tttcaggaag
     6781 tgcgccacca acctttcagt acgaaaattg ctttcattgt cgataccctt tttacgtgaa
     6841 cttgcgtact agttaactag ttcgatgatt aattgtcaac agctcatttc agaatatttg
     6901 ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag aacgggagtg cgccttgagc
     6961 gacacgaatt atgcagtgat ttacgacctg cacagccata ccacagcttc cgatggctgc
     7021 ctgacgccag aagcattggt gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc
     7081 accgaccatg acaccacagc tgcaatcgcg ccagcaagag aggaaatttc acgttcggga
     7141 ctggcgttga atcttattcc cggcgtggaa atttccacgg tctgggaaaa tcatgaaatt
     7201 catattgtcg ggctgaatat tgatattact catccgctaa tgtgtgagtt cctcgcgcag
     7261 cagacagaac ggcgcaatca gcgggcgcag ctgattgccg aaagacttga aaaagcgcaa
     7321 atacctgggg cgctggaagg cgcacaacga ctggcgcagg gcggcgcagt gacgcgcggt
     7381 cattttgcgc gtttccttgt tgagtgcggc aaagccagtt caatggcgga tgtctttaaa
     7441 aagtatctgg cgcgcgggaa aaccggatac gttccgccac agtggtgtac aatagaacaa
     7501 gctattgatg tcattcatca ttctggcggt aaggcggtgc tagctcatcc tgggcggtac
     7561 aatcttagtg ctaaatggct gaaaagattg gttgcgcatt ttgccgaaca ccacggtgac
     7621 gcgatggaag tcgcgcagtg ccagcaatcg cccaatgaac gtacccagct ggcggccctt
     7681 gcgcgtcagc atcatttatg ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg
     7741 atcgaactgg gtcgtaaact ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg
     7801 gaacagccgc agaacaccac agagagggaa ttatgagcca gtttttttat attcatcctg
     7861 ataacccaca gcaacgtctg atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga
     7921 ttgtttatcc aactgattcc ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga
     7981 tggagcgtat ttgtcgtatt cgccagctgc cggacggtca caactttacc ctgatgtgtc
     8041 gcgatctttc tgaactctcg acctattcat ttgttgataa cgttgcgttt cgtttaatga
     8101 aaaacaacac gccgggcaac tataccttca tcttgaaggg gacgaaggaa gtgccacgcc
     8161 gtctgttgca ggaaaaacgc aaaaccatcg gtatgcgtgt gccttctaac ccgattgcgc
     8221 aggcgttgct tgaagcactg ggcgaaccga tgctttccac ttcgctaatg ctgccaggca
     8281 gcgaatttac cgaatccgat cccgaagaaa ttaaagaccg tctggaaaaa caggtggatt
     8341 tgattatcca cggcggctat ctcggacaga aaccgacaac ggttattgat cttaccgatg
     8401 atacgccggt agtggtgcgt gaaggcgtag gtgatgtgaa gcctttctta taagtgcgta
     8461 actctgtata ctacgcggcc ttaaaatggc gctatacggc gccattgatt ctcctgttcg
     8521 acgcctgtga aggcgacacc aaaaggaagc tctatgagcg aaaaattaca gaaagtgctg
     8581 gcccgcgctg gccacggctc ccgccgtgaa atcgaatcta ttattgaagc cggtcgtgtg
     8641 agtgttgatg gcaaaattgc taaactcggc gatcgcgttg aagttacccc tgggctgaaa
     8701 attcgtatcg atggtcatct gatttcggta cgtgagtccg ctgaacaaat ttgtcgcgtt
     8761 ctggcctatt acaaaccgga aggtgagttg tgtacccgta acgatccgga agggcgtccg
     8821 acggtgtttg accgtctgcc aaagctgcgt ggcgcacgct ggattgccgt aggtcgtctg
     8881 gacgttaaca cctgtggtct gctgctgttc accaccgatg gtgaactggc aaaccgttta
     8941 atgcatccaa gccgtgaagt tgaacgtgaa tacgccgtgc gtgtatttgg tcaggttgac
     9001 gacgcgaaac tgcgtgattt gagtcgtggc gtgcaactgg aagatggtcc ggcagctttt
     9061 aaaaccatca agttcagcgg cggcgaaggg atcaaccagt ggtacaacgt gactctgacc
     9121 gaaggccgta accgtgaagt tcgtcgtctg tgggaagcgg ttggtgtgca ggtgagccgc
     9181 ctgatccgtg ttcgttacgg tgatatccca ctgccaaaag gtctgccacg cggtggctgg
     9241 acggagctgg acctcgccca gactaactat ctgcgcgaac tggtggagct tccgccagaa
     9301 accagctcta aagtcgctgt agaaaaagac cgtcgtcgca tgaaggctaa tcagattcgt
     9361 cgtgcggtga aacgtcacag tcaggtgagc ggcggtcgtc gttctggcgg gcgtaataac
     9421 ggttaatcaa agtatgccgg acgtcatatc cggcattttt acagattaat aatcaatccc
     9481 tatttgcgcc ttaacaccgg catcaaacgc atgtttgatg gggcgcagct cacttaccgt
     9541 atccgccagt tcaagaatat cccgatgaca gcctcgaccc gtgataatca ccgtctgttg
     9601 atgtggacgt tcatttaacg cctgcaccac ttcttccagt ggcaaatagt catacgccac
     9661 catatacgtc agttcatcaa gcaaaaccat atccagtgag gaatcagcaa gcatccgctt
     9721 tgcatgttgc cagacttcgc ggcaggcggc ggtatcagac tcgcggtttt gtgtatccca
     9781 ggtaaagccc gttgccatca cctgaaactc aacgccatgt ggctccagca gattgcgttc
     9841 gccattaggc caggtgcctt taataaactg cacgacgccc actttttttc cgtgaccaac
     9901 tgcgcgtgtt gccgtaccaa atgccgcggt ggttttgcct tttccattgc cggtaaagac
     9961 gatgataata ccgcgttcat cctgggcctg ggccacacga gcatctactt tttctttcac
    10021 tcgctgctga cgctgttggt agcgttcatc actcattggg aaattcctgg tttacggccc
    10081 ggttgggcgt caaaggtcat gccggtttta cggcggctgt catcgcccat cagccagagg
    10141 tagagcggca tgatatcagc gggtgtttta agtttctgtg gatcttcggt cgggaaggcg
    10201 cttgcgcgca ttgcggtgcg cgtaccgcct gggttaatgc agttgacgcg caggcgctgc
    10261 tgatattcat cggccagtac ctgcatcatc ccttcggtgg caaatttcga cgctgcatat
    10321 gcaccccagt tagctcgtcc ctgacgtcca acgcttgatg aagtaaagac cagtgaaccg
    10381 gcgtccgatt tgagtaataa aggaagcagt gcctgggtga gcataaaggt ggcattaacg
    10441 ttgacctgca tgacgtcctg ccagacctgc ggattttgtt cgctcattgg gcaaatatcg
    10501 ccgagcaatc cggcattatg caaaacacca tccagacgcg gataattaac gacaatgcgc
    10561 tgtgccagtt gttggcaatc ttcggacgtg caggtcagca aatcgagaat aaaccactgt
    10621 ggctgacgtc cagtttcttc gtttatgtgg ctggctacct gacgtaattt ttcttcatta
    10681 cggcccaaca gaatcactgt cgcaccatag cgtgcatacg tcatcgcggc ttcacggcca
    10741 atcccatcgc tggctcccgt caccaggata atgcgatcat tgagtaaatc ttgttttggc
    10801 tggtaatgca tggctactcc tcaacgacgt tgtctgtcgc gctgccagtg tactttatga
    10861 ctttggggct ttatgcctga aacagaaggg catttcaatc agcctggggc gataacaccg
    10921 caaaatggat actttgtcat actttcgctg caataacatc tctgcgagac ggcttaacat
    10981 gcctgttgta aactgtgagc caaagcgttg tttaaccaag gtggggactc gtggaattgt
    11041 tgtctgaata tggtttgttt ttggcgaaaa tcgttaccgt tgtgctagcg attgcggcga
    11101 ttgccgccat tattgtcaat gttgctcaac gtaataaacg ccagcgtggc gagttacggg
    11161 tcaacaatct cagcgaacag tataaggaga tgaaagaaga actggccgcg gcgctgatgg
    11221 actcacatca gcaaaaacag tggcacaaag cgcagaagaa aaagcacaag caagaagcga
    11281 aagcagcaaa agcgaaagcc aaactgggcg aggtggcaac tgacagtaaa cctcgcgtct
    11341 gggtgctgga ttttaaaggc agcatggacg cccatgaagt gaactcgcta cgtgaagaga
    11401 taacggctgt actcgcagca ttcaaaccgc aggatcaggt tgtgctacgt ctggaaagcc
    11461 ctggtggcat ggtgcatggt tacgggttgg cggcttcgca gctgcagcgt ctgcgtgata
    11521 aaaacattcc tttaactgtt acggtagaca aagtcgctgc cagcggcggt tacatgatgg
    11581 cctgtgtggc ggacaaaatt gtttccgcac cgtttgctat tgtgggttcc attggggtgg
    11641 tggcgcaaat gcccaacttt aaccgcttcc tgaaaagcaa agatattgat atcgaactgc
    11701 acactgccgg gcagtataag cgtactctga cgttgctggg tgaaaatacc gaagaagggc
    11761 gggagaaatt ccgcgaagag ttgaacgaaa cgcatcagtt gtttaaagat tttgtgaagc
    11821 gtatgcgtcc gtctctggat attgaacagg tggcaacggg tgaacactgg tacggacaac
    11881 aggcggtaga gaaaggcctg gttgatgaaa tcaacaccag tgatgaagtt attcttagcc
    11941 tgatggaagg ccgtgaagtg gttaatgtac gctatatgca gcgtaaacga ctcattgacc
    12001 gattcaccgg cagcgcggca gagagcgccg atcgattgtt gctacgctgg tggcagcggg
    12061 gtcaaaagcc attgatgtaa aagacaaacg cgaggctaag acctcgcgtt ttgctttaat
    12121 caaccagatg atatttttct gaaagcacat gggccaggtg tttgaacata ttaaacaccg
    12181 cggtgctttt ggctgttggc aatccttgtt catctaaaaa gtagtcgccg gtaaatacca
    12241 gcacgccatt acgctgcgtg acgccggtgg cttcaatccc tgcgagcgta tcctcatgct
    12301 cacgaatgat tttgttggcc tctttcaaca gcgtttcgcg gtcgatgggt tgtgtctctt
    12361 tgttcattat ttactcctta aacaaggaca ttagtctacg ccaggcatgg cttgcagaca
    12421 aatataccac gctggtggca agagcgcctt actggcaact ttggattttg catgctaata
    12481 aagttgcgta tcggatttta tcaggtacag tgtgacgctt tcgtcaatct ggcaatagat
    12541 ttgcttgaca ttcgaccaaa attccgtcgt gctatagcgc ctgtaggcca agacctgtta
    12601 actcagtcac ctgaattttc gtgaacagag tcacgacaag gggttgatat ccgcagagaa
    12661 cgagtccata tcggtaactc gttgccagtg gaaggtttat caacgtgcga cgcattcctg
    12721 gaagaatcaa attaggtaag gtgaatatgg gtaaagctct tgtcatcgtt gagtccccgg
    12781 caaaagccaa aacgatcaac aagtatctgg gtagtgacta cgtggtgaaa tccagcgtcg
    12841 gtcacatccg cgatttgccg accagtggct cagctgccaa aaagagtgcc gactctacct
    12901 ccaccaagac ggctaaaaag cctaaaaagg atgaacgtgg cgctctcgtc aaccgtatgg
    12961 gggttgaccc gtggcacaat tgggaggcgc actatgaagt gttgcctggt aaagagaagg
    13021 tcgtctctga actgaaacaa ctggctgaaa aagccgacca catctatctc gcaaccgacc
    13081 ttgaccgcga aggggaagcc attgcatggc acctgcggga agtgattggg ggtgatgatg
    13141 cgcgctatag ccgagtggtg tttaacgaaa ttactaaaaa cgcgatccgc caggcattta
    13201 ataaaccggg tgagctgaat attgatcgtg ttaatgccca gcaggcgcgt cgctttatgg
    13261 accgcgtggt ggggtatatg gtttcgccgc tgctatggaa aaagatcgct cgtggtctgt
    13321 ctgccggtcg tgtgcagtcg gtggcggttc gcctggtggt cgagcgtgag cgtgaaatta
    13381 aagcgttcgt gccggaagag ttctgggaag tcgatgccag cacgaccacg ccatctggtg
    13441 aagcgttggc gttacaggtg actcatcaga acgacaaacc gttccgtccg gtcaacaaag
    13501 aacaaactca ggctgcggta agtctgctgg aaaaagcgcg ctacagcgtg ctggaacgtg
    13561 aagacaaacc gacaaccagt aaacctggcg ctccttttat tacctctacg ctgcaacaag
    13621 ctgccagcac ccgtcttgga tttggcgtga aaaaaaccat gatgatggcg cagcgtttgt
    13681 atgaagcagg ctatatcact tacatgcgta ccgactccac taacctgagc caggacgcgg
    13741 taaatatggt tcgcggttat atcagcgata attttggtaa gaaatatctg ccggaaagtc
    13801 cgaatcagta cgccagcaaa gaaaactcac aggaagcgca cgaagcgatt cgtccttctg
    13861 acgtcaatgt gatggcggaa tcgctgaagg atatggaagc agatgcgcag aaactgtacc
    13921 agttaatctg gcgtcagttc gtagcctgcc agatgacccc agcgaaatat gactccacga
    13981 cgctgaccgt tggtgcgggc gatttccgcc tgaaagcacg cggtcgtatt ttgcgttttg
    14041 atggctggac aaaagtgatg cctgcgttgc gtaaaggcga tgaagatcgc atcttaccag
    14101 cagttaataa aggcgatgct ctgacgctcg ttgaacttac accagcccag cactttacca
    14161 agccgccagc ccgtttcagt gaagcatcgc tggttaaaga gctggaaaaa cgtggtatcg
    14221 gtcgtccgtc tacctatgcg tcgatcattt cgaccattca ggatcgtggc tacgtgcgag
    14281 tagaaaatcg tcgtttctat gcggaaaaaa tgggcgaaat cgtcaccgat cgccttgaag
    14341 aaaatttccg cgagttaatg aactacgact ttaccgcgca gatggaaaac agcctcgacc
    14401 aggtggcaaa tcacgaagca gagtggaaag ctgtactgga tcacttcttc tcggatttca
    14461 cccagcagtt agataaagct gaaaaagatc cggaagaggg tggtatgcgc ccgaaccaga
    14521 tggttctgac cagcattgac tgcccgactt gtggtcgcaa aatggggatt cgcacagcga
    14581 gcaccggggt attccttggc tgttctggtt atgcgctgcc gccgaaagag cgttgcaaaa
    14641 ccaccattaa cctggtgccg gaaaacgaag tgctgaacgt actggaaggc gaagacgctg
    14701 aaaccaacgc gctgcgcgca aaacgtcgtt gcccgaaatg cggcacggcg atggacagct
    14761 atctcatcga tccgaaacgt aagttgcatg tctgtggtaa taacccaacc tgcgacggtt
    14821 acgagatcga agagggcgaa ttccgcatta aaggttatga cggcccgatc gttgagtgtg
    14881 aaaaatgtgg ctctgaaatg cacctgaaaa tggggcgttt cggtaaatac atggcctgca
    14941 ccaacgaaga gtgtaaaaac acacgtaaga ttttacgtaa cggcgaagtg gcaccaccga
    15001 aagaagatcc ggtgccatta cctgagctgc cgtgcgaaaa atcagatgct tatttcgtgc
    15061 tgcgtgacgg tgctgccggt gtgttcctgg ctgccaacac tttcccgaaa tcgcgtgaaa
    15121 cgcgtgcgcc actggtggaa gagctttatc gcttccgcga ccgtctgccg gaaaaactgc
    15181 gttatctggc cgatgcgcca cagcaggatc cggaaggtaa taagaccatg gttcgcttta
    15241 gccgtaaaac caaacagcaa tatgtctctt cggaaaaaga cggaaaggcg actggctggt
    15301 cagcatttta tgttgatggc aaatgggttg aaggaaaaaa ataaccttta attctgtcag
    15361 gtttttataa acaaagggtc gcgaaagcgg ccctttttta ttgcatatta ttttttcttc
    15421 acacctatac actaaggcta taaatgatat agtggttata gttagcacct tttttattat
    15481 taaatcgtat tagtcacccg ccaggtgtga cgaaaaaacg atgttctgat ggcgtctaag
    15541 tggatggttt aacatgaaat tacaacaact tcgctatatt gttgaggtgg tcaatcataa
    15601 cctgaatgtc tcatcaacag cggaaggact ttacacatca caacccggga tcagtaaaca
    15661 agtcagaatg ctggaagacg agctaggcat tcaaattttt tcccgaagcg gcaagcacct
    15721 gacgcaggta acgccagcag gacaagaaat aattcgtatc gctcgcgaag tcctgtcgaa
    15781 agtcgatgcc ataaaatcgg ttgccggtga gcacacctgg ccggataaag gctcgctgta
    15841 tatcgccacc acgcataccc aggcacgcta cgcgttacca aacgttatca aaggctttat
    15901 tgagcgttat ccccgcgttt ctttgcatat gcaccagggc tcgccgacac aaattgctga
    15961 tgccgtctct aaaggcaatg ctgatttcgc tatcgccaca gaagcgctgc atctgtatga
    16021 agatttagtg atgttaccgt gctaccactg gaatcgggct attgtagtca ctccggatca
    16081 cccgctggca ggcaaaaaag ccattaccat tgaagaactg gcgcaatatc cgttggtgac
    16141 atataccttc ggctttaccg gacgttcaga actggatact gcctttaacc gcgcagggtt
    16201 aacgccgcgt atcgtcttca cggcaacgga tgctgacgtc attaaaactt acgtccggtt
    16261 agggctgggg gtaggggtca ttgccagcat ggcggtggat ccggtcgccg atcccgacct
    16321 tgtgcgtgtt gatgctcacg atatcttcag ccacagtaca accaaaattg gttttcgccg
    16381 tagtactttc ttgcgcagtt atatgtatga tttcattcag cgttttgcac cgcatttaac
    16441 gcgtgatgtc gttgatgcgg ctgtcgcatt gcgctctaat gaagaaattg aggtcatgtt
    16501 taaagatata aaactgccgg aaaaataatt tcttgcgtta ttttcggcac cttttatgta
    16561 gcgaaggtgc cggaatatat tctcttttgt tacttatttc tataccgtgc acaat
//
LOCUS       DAZFFI010000096        16609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFFI010000096 DAZFFI010000000
VERSION     DAZFFI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.096"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..191)
                     /locus_tag="ACHJ12_003708"
     CDS             complement(39..191)
                     /locus_tag="ACHJ12_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4991435.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(456..1190)
                     /gene="cysH"
                     /locus_tag="ACHJ12_003709"
     CDS             complement(456..1190)
                     /gene="cysH"
                     /locus_tag="ACHJ12_003709"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4991436.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1264..2976)
                     /gene="cysI"
                     /locus_tag="ACHJ12_003710"
     CDS             complement(1264..2976)
                     /gene="cysI"
                     /locus_tag="ACHJ12_003710"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4991437.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2976..4775)
                     /gene="cysJ"
                     /locus_tag="ACHJ12_003711"
     CDS             complement(2976..4775)
                     /gene="cysJ"
                     /locus_tag="ACHJ12_003711"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4991438.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5094..5456
                     /gene="queD"
                     /locus_tag="ACHJ12_003712"
     CDS             5094..5456
                     /gene="queD"
                     /locus_tag="ACHJ12_003712"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4991439.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5534..6805
                     /gene="ygcN"
                     /locus_tag="ACHJ12_003713"
                     /pseudo
     CDS             5534..6805
                     /gene="ygcN"
                     /locus_tag="ACHJ12_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            6796..7056
                     /gene="ygcO"
                     /locus_tag="ACHJ12_003714"
     CDS             6796..7056
                     /gene="ygcO"
                     /locus_tag="ACHJ12_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4991440.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7073..7648
                     /gene="ygcP"
                     /locus_tag="ACHJ12_003715"
     CDS             7073..7648
                     /gene="ygcP"
                     /locus_tag="ACHJ12_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4991441.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7796..8656)
                     /gene="ygcQ"
                     /locus_tag="ACHJ12_003716"
     CDS             complement(7796..8656)
                     /gene="ygcQ"
                     /locus_tag="ACHJ12_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4991442.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8653..9432)
                     /gene="ygcR"
                     /locus_tag="ACHJ12_003717"
     CDS             complement(8653..9432)
                     /gene="ygcR"
                     /locus_tag="ACHJ12_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4991443.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9410..10819)
                     /gene="ygcS"
                     /locus_tag="ACHJ12_003718"
     CDS             complement(9410..10819)
                     /gene="ygcS"
                     /locus_tag="ACHJ12_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4991444.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10841..12295)
                     /gene="ygcU"
                     /locus_tag="ACHJ12_003719"
     CDS             complement(10841..12295)
                     /gene="ygcU"
                     /locus_tag="ACHJ12_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4991445.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12365..13150)
                     /gene="ygcW"
                     /locus_tag="ACHJ12_003720"
     CDS             complement(12365..13150)
                     /gene="ygcW"
                     /locus_tag="ACHJ12_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4991446.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13469..14746
                     /gene="yqcE"
                     /locus_tag="ACHJ12_003721"
     CDS             13469..14746
                     /gene="yqcE"
                     /locus_tag="ACHJ12_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4991447.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14773..16251
                     /gene="ygcE"
                     /locus_tag="ACHJ12_003722"
     CDS             14773..16251
                     /gene="ygcE"
                     /locus_tag="ACHJ12_003722"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4991448.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3956 a         4369 c         4179 g         4105 t
ORIGIN      
        1 cacaattcag tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg
       61 agaactgcct taaactccat accacgttct ctgatactca actcacacaa cgagtcacct
      121 accatcagcg tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat
      181 tttgtcagca tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc
      241 gaacgtaagc ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg
      301 cgaatgctta acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt
      361 ccagcggcgt tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca
      421 tttctgacag aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc
      481 ctttttaagc caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt
      541 gtatgggtat caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca
      601 tgtttttgca ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta
      661 aatacgccac gctgaattgc cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg
      721 cgcaggccag caaaccacgt ttgcgcattc agttctttca gcgcccggtt catcggttcg
      781 actttgttga tgtcattgta cttttcaatg ccttcaacgc cctgttccca cagtttgccg
      841 tagcgtgctt cctgccaggc ggcgctttcg gtagcgcggt acactttcag gttgagcttg
      901 agtttgtccg ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtgtcg
      961 gtgaggatca ccggaatatc cgggtgaatc tgattcacca gatgcaggct caccgccgcc
     1021 tgaatgccaa agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggca
     1081 acgcggcctt cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg
     1141 cgatctacct tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca
     1201 ctgtttgcct tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga
     1261 tggttaatcc cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac
     1321 cgtaaagtcg ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc
     1381 atcaagcgac gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg
     1441 tgtcccaatg cggttgccgc caagatgcag attgtagcga cccggtgctt tacccaccag
     1501 gcccacttcc gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac
     1561 gatatgctca tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga
     1621 cggcaggaaa cgctccgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc
     1681 catcgagttt tcacgctgcg gcgtgacggc attcattaaa ccgctctctt tggcgatctt
     1741 ctcgatcttc gctttctcgc tttccggtac accggcgatg atcagattct ggttcgccgt
     1801 aatgcggaaa tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg
     1861 ccccggataa tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc
     1921 aatgccctta acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg
     1981 ttcaaatttg atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg
     2041 ctccagcgtg tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg
     2101 agttgtcacg acggcttcgg ccaccgccag cgtatgctcc agcggcagat agccaaactc
     2161 gctcgccgtg cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac
     2221 cagcaggttg aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt
     2281 ggcatgcaga tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg
     2341 caggtaggtc tgaccaagga tcggttcttc atcagtagtg gcgacttttt cctggtcgag
     2401 ccagatctcc gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca
     2461 ctcgtacgct tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg
     2521 gttcatgtcg ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac
     2581 cggtttgacg ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg
     2641 aatgctgcca tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt
     2701 ggtaatcacc ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt
     2761 ctgttcagca cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg
     2821 gatcagcagg aagttgtcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc
     2881 aatggtgccg cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc
     2941 ttcgaccact aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg
     3001 cgctctacgc gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat
     3061 tcggcaatca cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg
     3121 cagacataaa tgtgggcacc atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc
     3181 agtttgtctt gtacgtaaac tttttctttt tggtcgcgcg accaggcaag atcgatacgt
     3241 gtcagcacgc catctttgac gtagcgctgc cactccacct gatacaggaa gtcttccgta
     3301 aagtgcggat taccaaagaa cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc
     3361 tgcataaagg cgcggaacgg cgcaataccg gtgcctgggc caatcataat caccggggtt
     3421 tccgggttag cgggcaggcg aaagttatcg ttatgttcga taaatacgcg gacttcgccc
     3481 tcttcttcca cgcgatccgc gaggaagctg gaggcaccac cggcacgggc tcggccttcc
     3541 acgtcgtaac gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag
     3601 gaggcgatgg aatacaggcg cggggtcagc gggcgcagca gattaatcag cgcttcggca
     3661 tccagttgcg ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc
     3721 tgtaacttcg ctttatcgcc caccagcggc agcagtgttt cgctgcgggt aagcgtggcg
     3781 taattctcaa caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg
     3841 ttcagaggca acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt
     3901 tcgacaagtt ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc
     3961 ggctggtaac gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca
     4021 gagttacgcc cggtaatttt ctggttaaca gagaggctag ccaccagcgg cgcgtctttg
     4081 ctgtacgggc tggtgtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc
     4141 gcgacaggcg cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca
     4201 gcctggtatt caacatcggc atcgacacgg tcgagcaggc gttcaccacc cagttccgcc
     4261 agcttgctgt cgaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg
     4321 ctaaacacgg caaacgcggt gttttccagc tttggcgctt ttttggagaa caggaactta
     4381 tgcagcgcga cggcttcctc cggcggttcc ccttcccctt gcgttgacgt cactacgatg
     4441 agcagttttt cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg
     4501 ttcagttttg ctgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg
     4561 gtttgcgagg cggagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct
     4621 gcaagcgcag caggctgctg attgagcacg ccccagaaat agccagaaac ccaggcaagc
     4681 tgggtgggag ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac
     4741 ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa
     4801 gctgtttctg cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggtg
     4861 aacggtgtgg attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt
     4921 agcaatagtc tttaacaaca aaatagatta accaacctaa tgaaaaacaa atgaatttag
     4981 ccaatcatta agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa
     5041 aacagccttt ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca
     5101 ccacgttatt taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag
     5161 ggcataaatg tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg
     5221 aagtcgatcc gcatacgggc tggattatcg atttcgctga actgaaagcg gcgtttaagc
     5281 caacttatga gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa
     5341 ccagcgaagt tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa
     5401 gtgcggtgat ggtaaaagaa acctgtaccg caggttgtat ctatcgcggc gaatgataag
     5461 agtgtgtcgg cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata
     5521 tctggggcat gacatggaag acgactgcga cattattatt attggtgccg gtattgcagg
     5581 caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc
     5641 tgaaatcccc ggcagcaaaa atctttccgg cgggcggtta tatacccatg cactcgcgga
     5701 actcctcccg caatttcacc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct
     5761 ttccctgtta acgccggatg gtgcaacgac gttttccagc ttacagcccg gcggtgaatc
     5821 ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg
     5881 tgtcgaatgc atccccggtg cgacggtgga tgcgctgtat gaagaaaacg gcagggtgtg
     5941 tggtgtcatt tgtggtgacg atattctccg cgcccgttat gtggtgctgg cagaaggtgc
     6001 caacagcgtc ctggctgaac gtcacgggtt agtgactcgt cctgctggcg aagcgatggc
     6061 gttggggatc aaagaagtgc tgtcgctgga aacatccgct attgaagaac gttttcatct
     6121 ggagaataac gaaggcgcag cgttgctgtt cagcggcggg atctgtgatg acttacccgg
     6181 cggcgcattt ctttatacta atcaacaaac gctctcgtta gggattgttt gcccactctc
     6241 ttcccttacg caaagtcgtg ttccggcaag cgagctgctg gctcgcttta aaacgcatcc
     6301 ggcagtgcgc ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc
     6361 agaaggtggc ttgcacagta tgccggtgca atacgccggt aacggctggc tgctggtggg
     6421 cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct
     6481 gactggcgca caggcggcgg cacaaacgct gataagcgcc tgccagcacc gcgagccgca
     6541 aaatctgttt gcgctttatc atcacaacgt cgagcgcagc ctgctatggg atattctaca
     6601 gcgttatcag catgttccgg cgcttttgca acgccctggc tggtatcggg cgtggcctgc
     6661 gttaatgcag gatatttccc gcgatttatg agatcagggt gataaacctg ttccaccgct
     6721 gcgccagtta ttctggcgtc atttacgtcg tcatggcctg tggcatctgg cgggcgatgt
     6781 tatcaggagt ctgcgatgtc tgtagcccat aatctctggc gcgttgctga tgcgccgcac
     6841 attgttccgg ctgactccgt tgagcgtcag atggtacaac ggttgattaa cgcctgtccg
     6901 gcaggtcttt tttcgctcac accggaaggt gacttacgcg ttgattatca cggctgcctg
     6961 gagtgtggca cctgccgttt gctgtgcgac gaatccacac tacaacagtg gcgctatccg
     7021 ccttccggat tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct
     7081 cttacacctg ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca
     7141 actggctatc gattctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat
     7201 cagtaatatc gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt
     7261 gctggaaggc gcgtcaaata aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc
     7321 ggacggcatt atcagtacca aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt
     7381 ctgtattcat cgcctgttta ttgttgattc aatttcgttt cacaacattg ataagcaagt
     7441 tgcgcaatcg aatccggatt gtattgagat cctgccaggc tgtatgccca aagtgctggg
     7501 ctgggtgaca gagaaaatcc gccaaccgct gattgccggt gggctggtgt gcgatgaaga
     7561 agatgcgcgt aatgcgatta acgcgggtgt cgtggcgctt tccaccacga ataccggggt
     7621 ctggacgtta gcgaaaaaat tactttgacg ggataatcgt aaccaattga atttggtttg
     7681 atttgtaggc cgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt
     7741 atcaggccta caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt
     7801 ggcaatctgc gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca
     7861 cgccaacatc tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa
     7921 acttgctatt gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac
     7981 acacctcagg tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac
     8041 cgttcatcac tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag
     8101 cgatttcctg gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag
     8161 ctaatgggtc gcgagtaaca tttttcagat cctctgtgct aaccagccag tcgggaagcg
     8221 cacccgggac aatgttcagt tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc
     8281 ccgcctgacg cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta
     8341 gcgcatttcc ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct
     8401 ggcagatgct tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg
     8461 ttcccggcgg aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat
     8521 ccagcacctg ttccgccact acaggctgag gttcaatctg ccagtgcgcc agggtacaac
     8581 cgctgaaatc ctgcgccgcc agccagcttg ctattgcggc attctcctga tttattgtca
     8641 caattgcgat attcatggct gcatcctttg gcgtaaatag tcctgccata gcttttgagc
     8701 tttttctgct accgtttgcc catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc
     8761 cagttgcaga cactgcatcg ctggcatttc ggcagcaaca gttttacgga taatttctgc
     8821 tttccccgca gccattcgct ggcgcattcc cggtactggc aaggccactt caccgcactg
     8881 acgcacggca atcaccgcag gcaagcgaac ccggcaacaa cgcaaaccat gttcagtacg
     8941 ctgttcgagg gtgataaaca gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg
     9001 ccagcccagc atttccgcca gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg
     9061 gcagccggta attatcagat ccagcggatt ctgatgctgc cattcggcaa tatggcgagc
     9121 gacaaattcc ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa
     9181 cccaagagcc atgagatagc gcagccagtg caatgcccgt tcatccccca tgctcaacgc
     9241 ggttaaagac atcggcgtac cgttttttct ctgcgccaac agcagcgcgg cagcggcctg
     9301 ttcatcagca ccgagtaaac ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc
     9361 cgccgcctgc cactcttttt ccgccagcat tccggcatcc ggttcggctt taaacgctaa
     9421 cagaatgttc attcgcacct cctacatttc ccgccgccac cagcgggagg gctttagttt
     9481 ctggtgccca tagccaggtc acaacaaaac caaccaacaa cactgtcgcc aggagcaata
     9541 aggtgacttg cattccccac tgcgccagca cccacggcag caggccagta cttaccgccg
     9601 cgccaagtcg actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa
     9661 aactttccgc aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga
     9721 gaacaaaaag cagcagcgtt aatgaactgc cggaaggcaa acaggccatt actaccagcg
     9781 ttgccgccag cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca
     9841 gaactaatcc cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca
     9901 gcgcatcttc cagaccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc
     9961 atgggattac gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg
    10021 aagagaacaa ggttttgatg tgtttatggg tcgccgttac cacttcatcg cccagtaaaa
    10081 catggggacc aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca
    10141 gtagccagcg tggtgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca
    10201 gagctgccga tgccagtagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc
    10261 cggcaatact tgccagcaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa
    10321 taccgcgatg gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gaataatcgc
    10381 ctcccagacc aatgccaatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa
    10441 aaaattgtaa aaacgaagca agcgtaatca gcaaaaagct gaaggtgaag attttttgcc
    10501 gaccaatatg gtcggagatc cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg
    10561 ccgagccgcc gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagctgaa
    10621 taatggcgta accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga
    10681 gcgcagcaat gcggcagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag
    10741 tgttcatctt taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga
    10801 aaaggcaacg ccccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga
    10861 tagtacccgt attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg
    10921 cccacgcgct gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt
    10981 ggtgcaccat cgaaccgccg aggcgaatgg tttcttcaca gatgatcttg ttgagcggat
    11041 tgtggtactt gtcgatttcc tcttccggct tacagtcaac gacgttgtaa tcgtagacga
    11101 agtacatgtt ggtgccgttc tgatagctat gagaggaatg accgcccagc atggtgatgt
    11161 cgtcggcgtg cgggaactca gtacgaatac ggttaataac gctttcgtag atttcgtgga
    11221 tgcagctcca gcagccggac acttcagtgg taaagcccat gttgccggtt ttgaggatct
    11281 gcacacgttc ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga
    11341 tcagtttgct gtccacgcgc tggcactgcg ggtagcgggc aacgatttcc gcaatccctt
    11401 cgcccgtcgc cttcgcaatg cgaggattgc cttcagccat aaagatcagc acacatttac
    11461 cgtcggcaaa atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg
    11521 acggacgata cccttccacc atgacttcac gcaggatgtt gaagccggtt ttcatgtctt
    11581 ccaggatata gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta
    11641 cttcagtgat atagcaaagt gcaccttcgt taccgatgat gatgtggcga atgtccgggc
    11701 cagccgcgcg gcgtggcacg tttttaatgc gtgtgacggt gccatcagcc aatactgctt
    11761 ccagaccaac gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc
    11821 gggttgctac caggccgccc atctgcgcca gcggctttga ctgcggagaa tgccccgtgg
    11881 tgtaaccttt ttcacgcaac gcattttcca gcacctccag cggaacgcca cattgcgccg
    11941 tcgcctgcat attctcaata tcaatattaa tgatttgatt catggcggag ccgtcgagca
    12001 ccaccgagtt ttccacaacg gtttccagcc caccttcagt ggcggaagcg ccggtacgcg
    12061 gcacaccgtt aattttgtgc gcattcataa aattcagcac acgggagact tgttctgtgg
    12121 aaccgagttt tacgaccgct gccggaatcg gcaaagtata aataccatga atatccggaa
    12181 atttacgaaa acggtcaata ctgttcttct ttaatactgt ttcatcggta attacgcgat
    12241 ctgcaccaac aatttccttt agctggtcga caatcgctgc gcgagataaa gacatagtaa
    12301 ttccttcctg ataataaaaa tggaataatc attaataaga gaaaacagaa accaccgtta
    12361 tggattagcg cactaaataa ccaccatcga ccactaataa atgcccattg acataatttg
    12421 aagccggact tgcgaggaat acagctgcgc ccattaaatc ctgagtatcg ccccaacggt
    12481 ttgccggaat atgatcaaga acgcgctgat tggtttctgg attactgcgt gtcgccagcg
    12541 taatatctgt tgcataatag ccaggggcga taccatttac ctgaatatta tattgaccta
    12601 gctcatcaca ataagctttg gtgaacccgg caagagcatg tttagtggca gaatatgcag
    12661 gtgaccattg tccacctaag taagagaaca atgaacagat attaataatt ttgccgcttt
    12721 tctgcgggat cataattttt gcagcttcat agcttaactc gaatgcggcg gtcaggttca
    12781 catcaatcat cggatcccag tcggcacgac cgaagtccag caccttattc agcttacaaa
    12841 taccggcatt gtttaccaga atatcaactg taccgaaacg ctcacagcaa gcagcgataa
    12901 tcttctgcgg cgcgccttct gcggtgatat ccacctgcat gaagtccacc tcaacaccct
    12961 gtttttcaat catttccttt gtttcgccgt tatctttgac gaaactagga ataaagatat
    13021 ttgcgccagc tttggccaac gccatggcaa atgcctggcc taaaccgcta ttcccaccgg
    13081 taacaattgc ggttttacct ttcagtgaga aaaaatccat tgagaacgca ttgagagatt
    13141 cgattgacat agttagctcc aatttccgta aggcaaaaaa aaagaaaggt aacttccccc
    13201 gcgagaggga agttaccttt ctcattatct ctggtaactg gagataattt ttagcataac
    13261 ggcataactg ctaatgtgat cgtaatcaca gtgtgatatt cgtattgtca aatagtttta
    13321 aaaagcagaa gcgtgattac tctcacacaa cacctctgct ttccatgtta tttcttattg
    13381 caattactag agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt
    13441 ttttgtctat tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac
    13501 cctcgcgata attagtttta gcggcggcgt tagtttcgac ctggcttatt tacgttatat
    13561 ttatcaaatt cccatggcga aatttatggg attcagcaat accgagatag gtttaataat
    13621 gagtaccttt ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa
    13681 attttcacac cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt
    13741 aatggcaacg tatccaccgc tgtgggtaat gctctgtatt caggtcgcct ttgcgataac
    13801 gacgatttta atgctgtggt cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag
    13861 tgagcaaggg aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct
    13921 ggcggtgttt accatgtggg tcttttctcg ctttgcaccg gatgacagcg ccagcctgaa
    13981 aacagtcatt atcatctaca gtgtggttta catcttgttg gggattctgt gctggttttt
    14041 tgttagcgat aacaacaacc tgcgcagtgc caataacgaa gaaaaacagt cattccagct
    14101 tagcgacatc cttgccgttt tgcgtatcag caccacctgg tattgcagca tggtgatttt
    14161 tggtgtcttc acaatctacg ccattctgag ttactccacc aactatctga ccgaaatgta
    14221 cggcatgtcg ctggtggcag cgagctacat ggggattgtg atcaacaaaa tcttccgcgc
    14281 gctgtgcggc ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg
    14341 cgtgatccaa atcctttccg tactcggcct gctggcgtta actgccctgc tcgtcacgaa
    14401 ctctaacccg caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg
    14461 ttacgcctca cgcgggctgt actgggcctg ccctggcgaa gcgagaacac cgtcttacat
    14521 tatgggcacc acggtaggta tttgttcggt gattggattc ctgccggatg tcttcgttta
    14581 cccgattatc ggccactggc aagacaccct gcccgcagca gaagcctacc gcaatatgtg
    14641 gctgatggga atggcggcgc ttgccatggt gattgtcttt acctttttgc tgttccaaaa
    14701 aattcgtact gctgatagcg cccccgcaat ggctagcagc aagtaagcca ggaaaattac
    14761 aggagaaaaa tcatgtcgaa aaaatacatc atagggattg atggcggaag tcagagcaca
    14821 aaagtggtga tgtacgatct ggaaggtaac gtggtttgcg aaggcaaagg cttattacag
    14881 ccgatgcaca cgccagatgc cgatactgca gaacatcctg acgacgattt atgggcatca
    14941 ttatgttttg ccggtcacga tttgatgagt cagtttgccg ggaataaaga agatattgtc
    15001 ggtattggtc tgggatccat ccgttgctgt cgtgcgttat tgaaagccga tggcacgcct
    15061 gctgcgccgt tgattagctg gcaggatgca cgcgttacac gcccttacga acacactaat
    15121 cctgacgtgg catatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag
    15181 tttaaagaca atatcgccaa ctattttggt cagtggccgg tggattataa gagctggaca
    15241 tggagcgaag atgctgcggt aatggataag tttaatatcc cccgtcatat gctgtttgat
    15301 gtgcaaatgc ctggcaccgt cctcggacat atcacaccac aagccgcact ggcgacacat
    15361 ttcccggcag gactgccggt tgtttgtacc accagtgata aaccggtaga agctctgggg
    15421 gccggattac tggatgatga aactgcggta atttctcttg gcacttacat cgcattgatg
    15481 atgaacggca aagcactgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt
    15541 ccgcaaacat tgctgtatga aggttacggt attcgcaaag gtatgtggac ggtgagctgg
    15601 ctgcgtgaca tgttaggcga gtcgttaatt caggatgcca gggcgcagga tctttcaccg
    15661 gaagatttac tcaacaagaa agcctctagt gtgccacctg gctgtaatgg gctgatgacg
    15721 gtgctggact ggctgaccaa tccgtgggaa ccgtacaaac gcgggattat gatcggcttt
    15781 gattccagca tggattacgc atggatatat cgttcgatat tggaaagcgt ggcgctaacg
    15841 ctgaagaaca attacgacaa tatgtgtaat gaaatgaatc actttgcgaa gcatgtgatc
    15901 attactggcg gcggttcgaa cagcgatctg tttatgcaaa tttttgccga cgtgttcaac
    15961 cttccggcac gccgtaacgc cattaacggt tgtgcaagtc tgggagcagc gattaataca
    16021 gcggtaggtc tggggctata cccggattac gcaacggctg ttgataagat ggttcgcgtg
    16081 aaagatatct ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggc
    16141 attttcaaag acctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg
    16201 catggggaat tggggaatgt ggattcgatc cagagctggt cgaatgcgta agtgaggaag
    16261 gccgggcggg aaactgcccg gcctgaacat acctgaatgg ttatccccgc tgacgcgggg
    16321 aacataagtt cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgcagg
    16381 gaacataata aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact
    16441 acactcgatg tctttagaat aaaagacaat acgaatcgca ctttatcaat tttcacttaa
    16501 agtatgaact ggatatcttt attcataaga actattcatc aacagcgtat agaggcggtt
    16561 atgtaaaacc actcattagc ctcaaaacaa agacaaactt ccagattta
//
LOCUS       DAZFFI010000097        16176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFFI010000097 DAZFFI010000000
VERSION     DAZFFI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.097"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            309..1286
                     /gene="glaH"
                     /locus_tag="ACHJ12_003723"
     CDS             309..1286
                     /gene="glaH"
                     /locus_tag="ACHJ12_003723"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4991449.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1306..2574
                     /gene="lhgO"
                     /locus_tag="ACHJ12_003724"
     CDS             1306..2574
                     /gene="lhgO"
                     /locus_tag="ACHJ12_003724"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4991450.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGSEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2597..4045
                     /gene="gabD"
                     /locus_tag="ACHJ12_003725"
     CDS             2597..4045
                     /gene="gabD"
                     /locus_tag="ACHJ12_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4991451.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4059..5339
                     /gene="gabT"
                     /locus_tag="ACHJ12_003726"
     CDS             4059..5339
                     /gene="gabT"
                     /locus_tag="ACHJ12_003726"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4991452.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5650..7050
                     /gene="gabP"
                     /locus_tag="ACHJ12_003727"
     CDS             5650..7050
                     /gene="gabP"
                     /locus_tag="ACHJ12_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4991453.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7071..7733
                     /gene="csiR"
                     /locus_tag="ACHJ12_003728"
     CDS             7071..7733
                     /gene="csiR"
                     /locus_tag="ACHJ12_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4991454.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7734..8183)
                     /gene="kbp"
                     /locus_tag="ACHJ12_003729"
     CDS             complement(7734..8183)
                     /gene="kbp"
                     /locus_tag="ACHJ12_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4991455.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8267..8425)
                     /locus_tag="ACHJ12_003730"
     CDS             complement(8267..8425)
                     /locus_tag="ACHJ12_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4991456.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8608..8907
                     /locus_tag="ACHJ12_003731"
     CDS             8608..8907
                     /locus_tag="ACHJ12_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4991457.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNLYCP"
     gene            8917..9441
                     /gene="ygaP"
                     /locus_tag="ACHJ12_003732"
     CDS             8917..9441
                     /gene="ygaP"
                     /locus_tag="ACHJ12_003732"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4991458.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9488..9892)
                     /gene="stpA"
                     /locus_tag="ACHJ12_003733"
     CDS             complement(9488..9892)
                     /gene="stpA"
                     /locus_tag="ACHJ12_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4991459.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10559..11008
                     /gene="alaE"
                     /locus_tag="ACHJ12_003734"
     CDS             10559..11008
                     /gene="alaE"
                     /locus_tag="ACHJ12_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4991460.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(11045..11389)
                     /locus_tag="ACHJ12_003735"
     CDS             complement(11045..11389)
                     /locus_tag="ACHJ12_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4991461.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11541..11870
                     /locus_tag="ACHJ12_003736"
     CDS             11541..11870
                     /locus_tag="ACHJ12_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4991462.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            12118..12363
                     /gene="nrdH"
                     /locus_tag="ACHJ12_003737"
     CDS             12118..12363
                     /gene="nrdH"
                     /locus_tag="ACHJ12_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4991463.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            12360..12770
                     /gene="nrdI"
                     /locus_tag="ACHJ12_003738"
     CDS             12360..12770
                     /gene="nrdI"
                     /locus_tag="ACHJ12_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4991464.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12743..14887
                     /gene="nrdE"
                     /locus_tag="ACHJ12_003739"
     CDS             12743..14887
                     /gene="nrdE"
                     /locus_tag="ACHJ12_003739"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4991465.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14897..15856
                     /gene="nrdF"
                     /locus_tag="ACHJ12_003740"
     CDS             14897..15856
                     /gene="nrdF"
                     /locus_tag="ACHJ12_003740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4991466.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         3884 a         4079 c         4209 g         4004 t
ORIGIN      
        1 cggggcagat aatatgttta gtttactaac ggtcattttg cagtgaagcc atttactgtt
       61 ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt tgctttagtt
      121 attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta atttgttgct
      181 tttgatcaca ataagaaaac aatatgtcgc ttttgtgcgc atttttcaga aatgtagata
      241 tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat
      301 cgcttctgat gaatgcactg accgccgtac acaataacgc tgtcgattca ggccaggact
      361 atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca
      421 ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg
      481 agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc
      541 agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca
      601 atgcggtggg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg
      661 tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt
      721 tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtgatgg
      781 aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa
      841 tcgatgagca aaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac
      901 atctggacca ctatttccgc catccactgg cgcgtcgccc aatgcgcttt gccgcgccgc
      961 cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc
     1021 gcccggtgat gcgctatatc gaccagttcg tccagccgaa agacttcgaa gaaggcgtgt
     1081 ggttgagcga gctttcggac gccattgaaa ccagcaaagg cattctttct gtacccgttc
     1141 ccgttggcaa attcctgttg ataaacaacc tgttctggct gcacggtcgc gaccgcttta
     1201 ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg cggctacttc gcttacgcca
     1261 ctcaccacta ccagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg
     1321 attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga tgtctatccg
     1381 gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac
     1441 aacagcggcg tgatccatgc cggggtctat tacacgcccg gcagcctgaa ggcgcagttt
     1501 tgcctggcgg gaaaccgcgc caccaaagcc ttttgcgatc aaaacgacat tcgctatgac
     1561 aactgcggca agatgctggt cgccacgtcc gatctcgaaa tggaacggat gcgcgcgttg
     1621 tgggaacgca cggcggcgaa cggtatcgag cgcgagtggt taaacgccga ggaactgcgc
     1681 gagcgcgaac cgaatatcac cgggctcggc ggcatttttg tgccgtccag cggcattgtc
     1741 agctaccgcg aagtaacggc ggcgatggca aaaattttcc aggccagagg cagcgagatt
     1801 atctataacg ccgaagtcag cggccttagt gagcataaaa gcggcgtggt gatacgtacc
     1861 cgtcagggcg gcgactatga agcatcgacg ctgattagct gttccgggct gatggctgac
     1921 cggctggtga aaatgctcgg cctcgaaccg ggctttatca tctgcccgtt ccgcggcgag
     1981 tatttccgcc tagcgccgga gcataaccag attgttaacc atctgattta ccccattccc
     2041 gacccggcaa tgccgttttt gggcgtgcat ctcacccgaa tgatcgatgg cagcgtcacc
     2101 gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca
     2161 tttagcgaca cgctggagat tttgggctcg tcggggattc gccgggtgct gcaaaaccat
     2221 ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg
     2281 gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg
     2341 cgggcgcagg cggtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc
     2401 ccgcgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt
     2461 ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc
     2521 acgctgcgag cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg
     2581 aaaacaggac gtaacaatga aacttaacga cagtaactta ttccgccagc aggcgttgat
     2641 taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa
     2701 cggcgacaag ctgggtagcg tgccgaaaat gggcgctgat gaaacccgcg ccgctatcga
     2761 cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaacat
     2821 tctgcgcaac tggttcaatt tgatgatgga gcatcaggac gatttagcgc gcctgatgac
     2881 cctagaacag ggcaaaccac tggccgaagc gaaaggtgaa atcagctacg ccgcctcttt
     2941 tattgagtgg tttgccgaag aaggcaaacg catttatggc gacaccattc ccggtcatca
     3001 ggccgataaa cgcctgattg ttatcaagca gccgattggc gtcactgctg ctatcacgcc
     3061 gtggaacttc ccggcggcga tgattacccg taaagccggt ccggcgctgg cggcaggctg
     3121 caccatggtg ctgaagcccg ccagtcagac accgttctct gcgctggcgc tggcggagct
     3181 ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc
     3241 ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta ccggttcgac
     3301 cgaaattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga
     3361 gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga
     3421 aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt
     3481 atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa
     3541 actgcacatc ggcgacgggc tggataaagg cgtcaccatc gggccgctga tcgatgaaaa
     3601 agcggtagca aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt
     3661 ttgcggcggt aaagcggatg aacgcggcgg caacttcttc cagccgacca ttctggtgga
     3721 cgtgccggcc aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccgctgtt
     3781 ccgctttaaa gatgaagccg atgtgattgc gcaagccaat gacaccgagt ttggccttgc
     3841 cgcctatttc tacgcccgtg atttaagccg cgtcttccgc gtgggcgaag cgctggagta
     3901 cggcatcgtc ggcatcaata ccggcattat ttccaatgaa gtggccccgt tcggcggcat
     3961 caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat
     4021 caaatatatg tgcatcggtc tttaactgga gaatgcgaat gaacagcaat aaagagttaa
     4081 tgcagcgccg cagtcaggcg attccccgtg gcgttgggca aattcacccg attttcgctg
     4141 accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg
     4201 gcgggattgc ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc gcggtggaag
     4261 cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg
     4321 agctgtgcga gattatgaat cagaaggtgc cgggcgattt cgccaagaaa acgctgctgg
     4381 ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc gccaccaaac
     4441 gtagcggcac catcgctttt agcggcgcgt atcacgggcg cacgcattac acgctggcgc
     4501 tgaccggcaa ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc
     4561 gcgcgcttta tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc
     4621 accggatctt caaaaatgat gccgcgccgg aagatatcgc cgccatcgtg attgagccgg
     4681 ttcagggcga aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt ttacgcgcgc
     4741 tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta
     4801 ccggcacgct gtttgcgatg gagcagatgg gcgttgcgcc ggatctcacc acctttgcga
     4861 aatcgatcgc gggcggcttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggatg
     4921 ccgtcgctcc aggcggtctg ggcggcacct atgccggtaa cccgattgcc tgcgtggcgg
     4981 cgctggaagt gttgaaggtg tttgagcagg aaaatctgct gcaaaaagcc aacgatctgg
     5041 ggcagaagtt gaaagacgga ttgctggcga tagccgaaaa acacccggag atcggcgacg
     5101 tacgcgggct gggggcgatg atcgccattg agctgtttga agatggcgat cacagcaaac
     5161 cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg cgataagggc ctgattcttc
     5221 tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accgctcacc attgaagacg
     5281 ctcagatccg tcagggtctg gagatcatca gccagtgttt tgctgaggcg aagcagtagc
     5341 tatgtccgtt tgtaagagtt aaccgttgct aagggaatga taacgatgca gtaaaaaata
     5401 ataacgatga ggcgatcgac aaagttaaaa acgactgcgg actgtcccct cgcccctctg
     5461 gggagagggt tagggtgagg ggtaatcacg cggctacacc ctcaccccgg ccctctccct
     5521 aagggagagg gagaaaaccc tcccgctttc gtttttaaga ttttgttgac cgtctcaatt
     5581 aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     5641 ggattcagga tggggcaatc atcgcaacca catgagttag gcggcgggct gaagtcacgc
     5701 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     5761 gtcgccatcg ccgaagcggg cccggcggta ttactggcct atctgttcgc cggattactg
     5821 gtggttatga ttatgcgaat gctggcggaa atggcggttg ccacgcccga taccggttcg
     5881 ttttccacct atgccgataa agccattggc cgctgggcgg gttataccat cggctggctg
     5941 tactggtggt tttgggtact ggttatcccg ctggaagcca acatcgccgc tatgatcctg
     6001 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta
     6061 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     6121 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     6181 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     6241 aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     6301 gaaattgtca ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgct
     6361 accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtagtg
     6421 gcgttaatac cgtggaatat gccggggctg aaagccgttg gttcttatcg ctcggttctg
     6481 gaattgctca atattcccca tgcgaaatta atcatggact gcgtgatatt actttccgta
     6541 accagctgtc tgaactcggc gctgtatacc gcgtcaagga tgctctactc cttaagccgt
     6601 cgcggtgatg cgcccgcgct aatgggcaaa atcaaccgca gtaaaacccc gtatgtggcg
     6661 gtgttactct ccaccggagc ggcattttta acggtggtgg tgaactatta cgcacctgcg
     6721 aaagtgttta aattcctgat agacagctcc ggcgctatcg ccctgctggt ttatttagtc
     6781 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     6841 ttgcgcatgt ggctttatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     6901 ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta
     6961 gcgataggga ttatctgtac cgtgccaatt atggcgcgct ggaaaaagct ggtattgtgg
     7021 caaaaaacac ccgttcataa tacgcgctga tcatgatcag gagtcacacc atgaccatta
     7081 cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg
     7141 atgaaaaatt acgaatgagt ttgctgacat cgcgttatgc acttggcgtt gggccgttac
     7201 gggaagctct ttcgcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt
     7261 atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata
     7321 tggaagcgat gttagtgagt ctggcgattg cccgcggtgg cgatgagtgg gaggcagacg
     7381 ttctcgcaaa agcgcatctg ctgagtaagc ttgaggcctg tgacgccagc gagaaaatgc
     7441 ttgatgagtg ggatctgcgt catcaggcgt ttcatacggc aattgtggcg ggctgtggtt
     7501 ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta
     7561 tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac gatcagcacc
     7621 agaccctgac tgcggcggta ctggcgcgag ataccgcgcg cgccagtgag ttaatgcgcc
     7681 agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct
     7741 tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag catcggttta
     7801 ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca
     7861 ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt
     7921 ttcacctgat catcgacact ggcaataccg gaaatattcc ccaccgcaac aaggattttc
     7981 tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga
     8041 atattcactt tatcggcatc cggtataccg gttttgttca gatgctcctg caccttcttc
     8101 gcctgatcgt ctttatcgtg ctgacctgta accgcgtccc agagtttttc tccggcatct
     8161 ttcacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac tcagaaagtg
     8221 tagcagagga acgcggcgcg ttaacgccgc ggggatgagc acaggtttaa tcgcgggttt
     8281 gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcaac agaatattaa
     8341 taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gagcggcggc agaatgatgg
     8401 tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact cattgtttaa
     8461 aaagagattt tatctcttaa gcgtagtaat tttagcggag cctgccagtt tcagcttacg
     8521 cttttactct catttacgct gctgaggctg gtatatgttg catttttagt ttagagttta
     8581 cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca ggccagtgcc
     8641 gaacaggcag cggccttatt gaaagcaatg agccacccta aacgattgct gattctgtgc
     8701 atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac cggactgagt
     8761 gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat cgacagccaa
     8821 cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc cattatcgcc
     8881 accctgaaaa atctctattg tccgtaagga gtcactatgg ctttgacaac catttcgccg
     8941 catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg tgatgctgat
     9001 gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt gctggaacag
     9061 tcaggtcttc cggctaaatt acgtcacgag caaattatat tccactgcca ggcaggtaaa
     9121 cgcaccagta ataatgccga taaattagca gcgattgccg cccccgcaga aatattttta
     9181 ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt aaataaatct
     9241 caaccctttc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat attaatcggc
     9301 gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt tgttggtgcc
     9361 gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt agataagatg
     9421 ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg acatccggcc
     9481 tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc tgagcaattg
     9541 gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca tcggtgaatt
     9601 tatatttcgc cggacgcggc tggcgttttt taccagcgcg tggtgcagca gcagagctat
     9661 tacccaataa ctcttccggg ttaattccgt cagccttcat cagctccagc caggtgctaa
     9721 ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca cgtctttctt
     9781 tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag aattcgcgag
     9841 ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac ataataaaga
     9901 aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg atcacttatt
     9961 atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca gctgttattt
    10021 tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga gtaatttaga
    10081 ttattggtga acagagtggc gctctctgct cgctaattcc gcaagcctga caaaaccgaa
    10141 gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt gctttctgct
    10201 gttaatgtac attttctggc ataaaaaatg ctgtaaatga cacggtcacg acatacaccg
    10261 aattctctac gcttatagcg ttacctcacc cccaaacttt cattagtgaa attaaaggca
    10321 aggtcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa aatccacaat
    10381 gtacaaaaaa cgagccgtta cggaatattt atctacaaaa actgactaaa taaaaatttt
    10441 tcactaattg aatagtcata gccagcgata tacgctatgc gaaaatgcag atgacaatga
    10501 gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga gcattagcat
    10561 gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga tggttgttta
    10621 ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct tcgaacagtc
    10681 tttttattcc agattggtag cgattccggt gaacatctta attgcatggc catacggtat
    10741 gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct ggataaaaaa
    10801 tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt
    10861 agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca tcgttgtttc
    10921 gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac tgtttaaagt
    10981 cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat cgccagtcgc
    11041 gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca acacgcgaac
    11101 tgaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag agcggaaatt
    11161 gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt gaactgcgtt
    11221 cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg cgattaacat
    11281 agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca tcgacagtaa
    11341 aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata tttgatcctc
    11401 gttattatca accgcgggcc taccttacct gattgcgcat tcacaacgct ttcagaaaag
    11461 tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga aaattacatc
    11521 gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt gatgatggcg
    11581 tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct gtattgaagt
    11641 cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct caggcgttgc
    11701 tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc gcgcgcgatg
    11761 ccgttggctg cgcggattct tttgttcgtg aaagaccctg gtgtagcgtg ggtacagcag
    11821 ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag tgcaaaaatg
    11881 ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt tgcctggtga
    11941 atcacaaaaa tccccctacc ccgtcacgct catatccagg gtaatttcga ccactatttg
    12001 ctatatattg tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag
    12061 agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg
    12121 cgcattacta tttacactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg
    12181 gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcagcggaa
    12241 gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga tcttagctgg
    12301 tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc ggccagtgca
    12361 tgagccagct cgtctacttc tccagcagct ccgaaaatac gcagcgtttt atcgaacgtt
    12421 taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc
    12481 cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac
    12541 aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc gttattgctt
    12601 cgggtaatcg caactttggt gaggcgtatg gccgtgccgg agatgtgatt gctcagaaat
    12661 gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat atcgaaaacg
    12721 ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga cgcaggaaac
    12781 gatggattac cacgcgctga atgcgatgct taacctctac gatagcgcag gtcgcattca
    12841 gttcgataaa gaccgccagg ccgttgacgc ctttatggcg acgcatgtgc gtccgaacag
    12901 tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag gttactatga
    12961 tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg cccacgcaca
    13021 caccagcggt tttcgtttcc atacattcct cggggcatgg aagttttaca ccagctatac
    13081 gttgaagaca ttcgacggta agcgttatct ggaagatttt gccgatcgag tcacgatggt
    13141 ggcgctaacg ctggcacaag gcgatgaaac actggcgttg caactgaccg atgagatgct
    13201 gtcaggacgc tttcagcctg caacaccgac attcctcaac tgtggtaagc agcagcgcgg
    13261 cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga ttggccgggc
    13321 ggtaaattcc gcactgcaac tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa
    13381 tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtaattcc
    13441 ggtgatgaaa atgctggaag acgcattttc ctatgccaac caactcggcg ctcgtcaggg
    13501 ggctggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgacacgaa
    13561 acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgcttggcg tggtgatccc
    13621 ggatatcact ttccatctgg ccaaagagaa tgcgcagatg gcgctgtttt cgccctatga
    13681 cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac actatgacga
    13741 actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt tcttccagcg
    13801 actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag acacggtaaa
    13861 ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctatgctcag aaattttgca
    13921 ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgtacag gccatgatat
    13981 ttcctgcaat ttaggttcgt tgaatattgc ccacaccatg gattccccca actttgcccg
    14041 cacggtagag actgccgtac gcggtttaac ggcggtgtca gatatgagtc atatccgcag
    14101 cgtgccgtcc atcgaagccg gaaatgccgc ctcacacgcc atcgggctgg ggcagatgaa
    14161 tttacacggt tatctggcgc gagaaggcat cgcttatggt tcgccggaag cactggattt
    14221 caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga tgttgctggc
    14281 acgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtagtgaata
    14341 tttcagccaa tatctgcaag ggaactggca gccgaaaacg acgaaagttg gcgaactgtt
    14401 tgcccgtagc ggtattacgt tacctacccg tgagatgtgg gcgcaactgc gcgacgacgt
    14461 gatgcgctac ggcatttata accagaatct tcaggcggtg ccgccaaccg gttctatctc
    14521 ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa
    14581 agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg agaatctggc
    14641 gctgtatcag gatgcttacg aaatcggcgc agaaaagatc atcgacacct acgcggaagc
    14701 gactcgccat gtcgatcagg gtttgtcgct gacgctgttt ttccccgata ccgccaccac
    14761 tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa cgctctatta
    14821 cattcgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc
    14881 actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc
    14941 tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa
    15001 ggtgccgctg tcgaacgata ttcctgcctg gcagacatta actgtcgtag aacaacaact
    15061 gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc
    15121 gccttcgctg atgcccgatg cactcacgcc tcatgaagaa gcggtattat cgaatatcag
    15181 ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc tgtgtcagac
    15241 caaagatgtc gatgccgcct acgcatggag tgaagaaaac gcaccgttgc agcgaaaagc
    15301 tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt
    15361 gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg
    15421 cggaaagctg accaatacag cagatctgat ccgtctgatt atccgcgatg aagcagtcca
    15481 cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc tgggacaacg
    15541 tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca acgagttgca
    15601 atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag cgtttctctg
    15661 ttacaacgcc aataaggctt tgatgaatct ggactacgaa ccgttatttc ccgcagaaat
    15721 ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga
    15781 tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa cagaagatga
    15841 agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc agggaaataa
    15901 ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac tgatactctg
    15961 ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta tgacaaaaaa
    16021 tatcaacttt tctcgatttg ctctcagccc ttatatcacg ggaaattccg gcgatttgct
    16081 cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc agtatgttag
    16141 ggtagaaaaa agtgactatt tccattgggt aatata
//
LOCUS       DAZFFI010000098        16147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFFI010000098 DAZFFI010000000
VERSION     DAZFFI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.098"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            194..346
                     /gene="mokC"
                     /locus_tag="ACHJ12_003741"
     CDS             194..346
                     /gene="mokC"
                     /locus_tag="ACHJ12_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4991467.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(450..1580)
                     /gene="dnaJ"
                     /locus_tag="ACHJ12_003742"
     CDS             complement(450..1580)
                     /gene="dnaJ"
                     /locus_tag="ACHJ12_003742"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4991468.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1669..3585)
                     /gene="dnaK"
                     /locus_tag="ACHJ12_003743"
     CDS             complement(1669..3585)
                     /gene="dnaK"
                     /locus_tag="ACHJ12_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4991469.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3962..4366
                     /locus_tag="ACHJ12_003744"
     CDS             3962..4366
                     /locus_tag="ACHJ12_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4991470.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4392..5105
                     /gene="msyB"
                     /locus_tag="ACHJ12_003745"
     CDS             4392..5105
                     /gene="msyB"
                     /locus_tag="ACHJ12_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4991471.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5254..5820
                     /gene="satP"
                     /locus_tag="ACHJ12_003746"
     CDS             5254..5820
                     /gene="satP"
                     /locus_tag="ACHJ12_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4991472.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGHIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5855..6442)
                     /gene="mog"
                     /locus_tag="ACHJ12_003747"
     CDS             complement(5855..6442)
                     /gene="mog"
                     /locus_tag="ACHJ12_003747"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4991473.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6557..7510)
                     /gene="tal"
                     /locus_tag="ACHJ12_003748"
     CDS             complement(6557..7510)
                     /gene="tal"
                     /locus_tag="ACHJ12_003748"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4991474.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKSLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7789..9219
                     /locus_tag="ACHJ12_003749"
     CDS             7789..9219
                     /locus_tag="ACHJ12_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4991475.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARFMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGTWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9289..10065
                     /gene="yaaA"
                     /locus_tag="ACHJ12_003750"
     CDS             9289..10065
                     /gene="yaaA"
                     /locus_tag="ACHJ12_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4991476.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKMTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10236..10622)
                     /gene="ridA"
                     /locus_tag="ACHJ12_003751"
     CDS             complement(10236..10622)
                     /gene="ridA"
                     /locus_tag="ACHJ12_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4991477.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(10695..11156)
                     /gene="pyrI"
                     /locus_tag="ACHJ12_003752"
     CDS             complement(10695..11156)
                     /gene="pyrI"
                     /locus_tag="ACHJ12_003752"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4991478.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(11169..12104)
                     /gene="pyrB"
                     /locus_tag="ACHJ12_003753"
     CDS             complement(11169..12104)
                     /gene="pyrB"
                     /locus_tag="ACHJ12_003753"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4991479.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(12108..12242)
                     /gene="pyrL"
                     /locus_tag="ACHJ12_003754"
     CDS             complement(12108..12242)
                     /gene="pyrL"
                     /locus_tag="ACHJ12_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4991480.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(12523..12918)
                     /locus_tag="ACHJ12_003755"
     CDS             complement(12523..12918)
                     /locus_tag="ACHJ12_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4991481.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(13049..13762)
                     /gene="bdcA"
                     /locus_tag="ACHJ12_003756"
     CDS             complement(13049..13762)
                     /gene="bdcA"
                     /locus_tag="ACHJ12_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4991482.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            13833..14426
                     /locus_tag="ACHJ12_003757"
     CDS             13833..14426
                     /locus_tag="ACHJ12_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4991483.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            14571..15023
                     /gene="tabA"
                     /locus_tag="ACHJ12_003758"
     CDS             14571..15023
                     /gene="tabA"
                     /locus_tag="ACHJ12_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4991484.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            15146..>16147
                     /locus_tag="ACHJ12_003759"
     CDS             15146..>16147
                     /locus_tag="ACHJ12_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4991485.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDGLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKFPYINDVRILDWCFNKRMQYF
                     "
BASE COUNT         3891 a         3999 c         3997 g         4260 t
ORIGIN      
        1 tcgataaaac agaatgggtt aaagagagcg atataatagc ggcgggtgct tgaggctatc
       61 tgtctcaggc attagctgaa cggcagatag agaaaagccc cgagtgatat tttactatca
      121 acccgaggcc ccctatatgc tgaacacatg tagagtgcct cttactgacc gtaaggtcaa
      181 ggagaagaga gcaatgaagc agcataaggc gatgattgtc gccctgatcg tcatctgtat
      241 caccgccgta gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc acatccgaac
      301 tggccagacg gaggttgctg ttttcacggc ttacgaatcc gagtaagtgc aacggcgggg
      361 agtgatcccc gccacttttc aggtgctcgc atatcttcaa cgcaccctaa ttttacccaa
      421 gcctgcccac gggcaggctt ttggggaggt tagcgggtca ggtcgtcaaa aaacttcttc
      481 acaccatcaa agaagctctt tgagcgcggg ctgttgtgct caccggttgg gccaccgaag
      541 ctttcttgca gctcttgcag cagctgtttc tgcttctcgt tcaggcctac cggtgtttcg
      601 acgacaacgc ggcacagcaa atcaccctgt gcgccaccgc ggacagactt gacgccttta
      661 ccgcgcatac ggaaaagctt accggtctgg gtttcgccag gcactttcag tttgacgcga
      721 ccatcaaggg tcggtacttc gatttcgcca cccagcgccg ccatagcgaa gttgatcggg
      781 acttcgcaat agaggttgtt gccttcacgc tcgaaaatcg ggtgctgttt aacctgaacc
      841 tgaacgtaca gatcgcctgc cggtgcgcca tgctcgcccg cttcaccttc gcccgcaaga
      901 cggatgcggt ctccagtgtc cacccctgcc gggattttaa cggacagcgt tttgctgcgc
      961 tcaacacgac catgaccatg acatttgttg cacggatctt tgatcagcgt accgcggccc
     1021 tgacagtgtg gacaggtctg ctgtacagcg aagaatccct gacgcatctg cacctgacca
     1081 gaaccatgac aggtcggaca ggtctgcggc tgtgtacctg gttttgcacc gctaccgtgg
     1141 caaacgtcac actcttccag agtcggaatg cggatctctt tggtcacgcc acgtacagct
     1201 tcttcgaggg tgagctccat gttatagcgt aaatcagcac cgcgcgccgc acgttgacga
     1261 ccacgtccgc cgccaaaaat atcgccgaaa acgtcaccaa aaatatcgct gaagtctgcg
     1321 ccgccgccaa aaccgccgcc gcccatgccg ccttgctcaa acgcagcatg accatactga
     1381 tcgtatgccg cacgtttttg cgagtcggtc agaacttcat aagcttcctt gatctcttta
     1441 aatttcgcct cggcctcttt gtcaccctgg ttacggtccg ggtggtattt catggccagg
     1501 cgtttgtagg cctttttgat ttcacgctct tccgctgttt tggaaacgcc taaaatctcg
     1561 taataatctt gcttagccat cttttttaaa ttgcccctag atgaatgcac gggcggagag
     1621 gaaattcccc ttcgcccgtg tcagtataat tacccgttta tagggcgatt attttttgtc
     1681 tttgacttct tcaaattcag cgtcgacaac atcgtcatct ttcgcgttgt ttgcagaagc
     1741 atcagcaccg gcagtctgct gctgggcatg ttgctgctgg gcgatttcca tcagtttctg
     1801 ggaaacctgt gccagctcct gcattttcgc ttcgatagcg gctttgtctt cacctttcag
     1861 agcagtttcc agtgcagtca gtgcagactc gatagcagtt ttgtcgtcag ccggcagttt
     1921 gtcgcctgct tcttcaacct gcttacgggt gctgtgcagc agatggtcgc cctggttgcg
     1981 agtctgtacc agctcttcaa acttacggtc agcttcggcg ttagcttctg cgtcgcgtac
     2041 cattttctgg atttcatctt cgttcagacc agaagaagcc ttgatggtga tcttctgctc
     2101 tttaccgctg tttttatctt tcgcggaaac gtgcaggata ccgtcagcat cgatatcgaa
     2161 ggtaacttcg atctgcggca tgccgcgcgg tgccgggttg ataccatcca ggttgaactg
     2221 acccagagat ttgttatcag ccgcacgttt acgttcaccc tgcagcacat ggatggttac
     2281 cgcagactgg ttgtcttcag cggtagagaa cacctggctg tgcttggtcg ggatagtggt
     2341 gtttttcgcg atcagcgtcg tcatcacacc gcccatggtt tcgataccca gagacagcgg
     2401 ggtaacgtcc agcagcagta cgtctttcac gtcaccagtc agaacaccac cctgaacagc
     2461 agcaccgatt gctacagctt cgtccgggtt aacgtcttta cgcggctctt taccaaagaa
     2521 ctcagcaact ttcttctgaa ccattggcat acgagtctga ccaccaacga ggataacgtc
     2581 gtcgatatca gatacggaca ggccagcgtc ctgcagtgca actttcagcg gctcaatgga
     2641 acggtttacc agatcttcaa ccaggctttc cagtttcgca cgagtcactt tgatgttcat
     2701 gtgtttcgga ccggtcgcat ctgcagtgat gtacggcagg ttaacgtcgg tctgctgagc
     2761 ggaagacagt tcgattttcg ctttttctgc cgcttctttc aggcgctgca ttgccagcgg
     2821 atcgttgcgc aggtcaatgc cctgatcttt cttgaattct tcaaccaggt agttgatcag
     2881 acggctgtcg aagtcttcac cacccaggtg ggtatcaccg ttggtagcca gaacttcgaa
     2941 ggttttttcg ccgtcaactt cgtcgatttc gataatagaa atatcgaaag taccaccacc
     3001 caggtcataa accgcgatag tacggttgcc agtacctttg tccagaccgt aagccagcgc
     3061 agctgcggtc ggttcgttga tgatacgttt tacttccaga ccagcgatac ggcctgcgtc
     3121 tttggttgcc tgacgctgag catcgttaaa gtatgccggt acggtgataa cagcttcagt
     3181 taccggttca cccaagtaat cttcagcggt tttcttcatt tttttcagca cttcagcaga
     3241 aatctgcggc ggtgccattt tctggccttt aacttcgacc catgcgtcgc cgttatcagc
     3301 agcaataatt ttgaacggca tgatggaaac atcacgctgt acttcttcgt cctggaagcg
     3361 gcggccaatc aggcgtttaa tcgcaaacag ggtgttttgc gggttcgtca ctgcctgacg
     3421 tttagccggc tgaccaacca gagtttcacc atcctgggta taggcaataa tagaaggcgt
     3481 ggtgcgatcg ccttcggcgt tctccagcac gcgaggagtg gtgccatcca taatcgctac
     3541 acaagagttg gtagtaccca ggtcgatacc aattatttta cccatctaaa cgtctccact
     3601 atatattcgg tcatcatgtg gttgtgagtc tgtaataggg gcgaaacgtc tggtttcaac
     3661 tgcccaattt catttttttg cagactcact cactgcggtt gactactaaa tggggtcgta
     3721 acccacgtca tcaaggggga gatgcaaaaa ttttttgtgc ttttgcgcag aaatttcgcg
     3781 atatagcagg acaagacgtg aaactgacag caattttatt catataaaga atgaatcgca
     3841 gccaagacat tcatcaaaaa attgtaataa aaagaaaaca ttacgcacct gaatcttctc
     3901 tttatcagca gtaaactagt gggtatccat ccccctacct ctccccacta agagaatcct
     3961 tatgaaatcc gtttttacga tttccgccag cctggcgatt agtctgatgc tgtgctgcac
     4021 ggcgcaggca aacgaccata aaatcctcgg cgtcattgcg atgccacgta acgaaaccaa
     4081 cgatctggcg ctgaaacttc ctgtttgccg aattgttaaa cgcatacaac tctctgccga
     4141 ccatggcgat ttacagttaa gcggtgcatc ggtttatttc aaagccgccc gtagcgccag
     4201 tcagagcctg aatattcctt cagaaataaa agaagggcaa accactgact ggatcaacat
     4261 taacagcgat aacgacaata aacgctgcgt ctcaaaaatc accttttccg gtcatacggt
     4321 gaactcatcg gatatggcca cgctgaaaat tatcggcgac gactaacgcc agaatataga
     4381 agccacaaaa aatgaatgtt aattacctga atgattcaga tctggatttt ctccagcatt
     4441 gcagtgagga acagttggca aatttcgccc gattgctcac ccataatgaa aaaggcaaaa
     4501 ctcgcctctc cagcgtactg atgcgcaacg aactgtttaa atctatggaa gggcatcccg
     4561 agcaacatcg ccgcaactgg cagctgattg ctggagaatt acagcatttt ggtggcgata
     4621 gtatcgccaa caaactgcgc ggacacggta aattgtatcg ggccattttg ctcgatgttt
     4681 caaaacgatt gaagctgaaa gccgacaaag agatgtctac gtttgagatt gagcaacagt
     4741 tactggaaca atttctgcgt aacacctgga agaatatgga cgaggaacat aaacaggagt
     4801 ttctgcacgc ggtcgatgcg agggtgaatg agctggaaga gctgctgccg ctgctgatga
     4861 aagacaaatt attggcaaaa ggcgtgtcgc atttgctttc cagccaactg acccgcattt
     4921 tacgcaccca cgcagcaatg agcgtacttg ggcatggttt gctgcgcggc gcggggctgg
     4981 gaggccctgt aggtgcggca ctaaatgggg ttaaagcggt cagcggcagc gcctatcgcg
     5041 tgacgattcc agccgtactg caaatcgcct gcctgcgccg gatggttagc gccactcagg
     5101 tctgataccg taaaccgggg aaaattttca ttttcccctc aaaagatcgt agacactgcc
     5161 ccactggctg attattatgc cgcgccctga aaacactaca gttattcagg gaaattattt
     5221 caccattcat tcgatgatga tttttgagga attatgggca acactaagtt ggctaatccg
     5281 gcaccgctgg gcctgatggg cttcggcatg accaccattc tgcttaacct gcacaacgtg
     5341 ggttatttcg ctctggacgg tattattctt gccatgggca ttttctacgg cggcatcgcg
     5401 caaatttttg ctggtctgct ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc
     5461 acctcttacg gttctttctg gctgacgctg gttgcgattc tgctgatgcc gaaactgggt
     5521 ctgaccgatg cgccaaatgc acagttcctt ggtgtctacc tgggtctgtg gggcgtattt
     5581 acgctgttta tgttcttcgg cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt
     5641 agcctgaccg tgctgtttgc cctgctggcg atcggtcaca ttgccggtaa cgccgcaatc
     5701 atccactttg ccggctggat tggtctgatc tgcggtgcca gcgcaatcta tctggcgatg
     5761 ggtgaagtac tgaacgagca gtttggtcgc accgttctgc cgattggtga atcccactaa
     5821 ttgttttgag atccccccgc tcggggggat ttttttattc gctaacgtcg cgtcttgcac
     5881 tcttcggtct gaatgcagca accacttctg gtgccgtttc aacgtatggc ccttccagca
     5941 actgaatgca gtacggtacg ctggcaaaaa taccgtgtac cacaacgtta ccctcagcgt
     6001 ccttcacacc ttccagcgtc tctttaatag acttcggctg gccgggtaag ttaaggatca
     6061 gcgcctgttt gcgaatcacg cccacctgac gcgaaaggat cgcagttggt acaaaatgca
     6121 ggctgatctg gcgcatctgt tcaccaaagc caggcatctc gcggtccgct actgccagcg
     6181 tcgcatcggg cgttacgtca cgacgcgccg ggccagttcc gcccgtggtg agcaccagat
     6241 ggcaactcat ttcatccacc agctcacaca acgtttgctc gatgatcgcc tgctcatcgg
     6301 gaattaagcg ggtttccagt tcaaacggcg tggttagcgc cgatgtcagc cattcttcca
     6361 gtgcagggat acctttatcc tgataaacgc cgctggatgc gcgatcagag atggaaacta
     6421 agccaatacg taaagtattc atcaagattt ttccgtttaa acagtctcgt taaacagaat
     6481 gatacactgc gaagggagtg acagacaggc ttcagaagag gtagcgtgac cgacttcccg
     6541 gtcacgctaa gaatgattac agcagatcgc cgatcatttt ttccagtttt tcctggtcaa
     6601 cagcaaactt acggatacct tccgccagtt tatctactgc cattggatcc tggttgtgct
     6661 gccacaggaa ctcggactca gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag
     6721 acagtttacg ttcaatagcc ccttcgctct cagccagctc tttcagcagt gccggtgcga
     6781 tggtcagacg gtcgcagcct gccagttcca gaatttcgcc gatgttacgg aagcttgcgc
     6841 ccataaccac ggtttcataa ccgtgctctt tgtagtactg gtagatttca gatacagaaa
     6901 ccacgcccgg atcttctgcc ggagcgtact ctttcttatc ggtattcgct ttgtaccagt
     6961 caagaatacg gccaacaaac ggcgagatca ggaacacgcc cgcttccgca caagcacgag
     7021 cctgagcgaa ggagaacagc agggtcaggt tacagttgat accttctttt tccagctgtt
     7081 ctgcagcacg aataccctgc caggtagaag ccagtttgat cagaatacga tcgttgctaa
     7141 taccagcatc gttgtagagt ttgatcaggc tttttgcttt cgcaattgac gcttcggtgt
     7201 cataggaaag acgcgcatca acttcagttg agatacggcc cggaaccagt ttcaggattt
     7261 ccagaccaat atttactgcc agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt
     7321 tgctctgctg tttcgcccag gcgacagcat catcaatcaa cttacggtat tccggaatct
     7381 gcgctgcgtt aagaatgaga gaagggttgg ttgtggcatc ctgcggttga tacagcttca
     7441 ttgccgcgat gtccccagtg tcggccacta cggtggtgta ctgacgaagg gaggtcaatt
     7501 tgtccgtcat gatagtattt ctctttaaac agcttgttag ggggatgtaa ccggtctgcc
     7561 ctgatgatat cacgacgcaa cgtcagcgca accgccgaca agacggttat cgccaggcga
     7621 gactgtttcg gatttctgac actgcgttga tatcgcccgc catttttata caaaacctca
     7681 tgtatgctac gcagaagtta tcaagtacct cgtagcgtat atacttctta aacaattggt
     7741 aacgtttaca caggaaagtc atcgcgaccg gcaataagag ggatatgcat gccagatttt
     7801 ttctccttta ttaacagcgt cctttgggga tcggtaatga tttacctgct cttcggcgca
     7861 ggttgttggt tcacttttcg caccggattt gtgcagtttc gctacatccg ccagtttggc
     7921 aaaagtctta aaaatagcat tcatccacag ccaggcggtt taacctcatt tcagtcattg
     7981 tgtaccagtc ttgcggcgcg cgtaggtagc ggcaacctgg ccggcgttgc gctggcaatt
     8041 accgccggtg gacctggagc cgtcttttgg atgtgggttg ccgcgtttat cggcatggcg
     8101 acctcgtttg ccgaatgttc ccttgcacaa ctttataaag aacgtgacgt caatgggcag
     8161 tttcgtggcg gaccggcatg gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt
     8221 ctgttcgccg tctttttgct catcgcctac ggcataattt tcagcggagt tcaggcgaac
     8281 gccgttgccc gagccctgag tttttctttt gattttcccc cgctggtgac aggcattatt
     8341 ctcgctgtct ttgctctgct ggcaatcact cgcggtcttc atggcgtcgc ccgcttcatg
     8401 caggggtttg tcccgttgat ggcgataatc tgggtactga ccagcctggt gatttgcgta
     8461 ataaatatcg ggcaacttcc ccacgtcatt tggtctattt ttgagagtgc ttttggctgg
     8521 caggaagcgg caggcggcgc ggcgggatat accttaagcc aggcgattac taacggtttt
     8581 cagcgcagta tgttttccaa tgaggcggga atgggttcga cgccaaacgc ggcagcggca
     8641 gctgcgtcct ggcctccgca tccggcagcg caagggattg tccagatgat tggcattttt
     8701 atcgacaccc tggtcatctg tacggcaagc gccatgctga tattactggc gggtaacggc
     8761 acaacctaca tgccgctgga aggtattcag cttatccaga aggcgatgcg ggtgctaatg
     8821 ggtacctggg gtgctgagtt tgttaccctc gtggttattc tgtttgcctt cagctccatc
     8881 gttgccaact acatttatgc cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa
     8941 gcgatctggt gtttgcggat ctgcaccttc gcaacggtca tcggcggcac cttgctaagt
     9001 cttccgctga tgtggcaact ggcagatatc ataatggcct gcatggctat taccaatttg
     9061 accgccattt tactgctctc gcctgtggtt cataccattg ccagtgatta tctacgccag
     9121 cgtaaactcg gcgtgcgccc ggtgtttgat ccgttgcgtt atccggatat cggtcgccag
     9181 ctttctccag acgcgtggga tgatgtttcg caggagtaat cacaactatc gatcaactca
     9241 ttctcttttt ttgctaaagt cggcataaat ttcctgcaag gactggatat gctgattctt
     9301 atttcacctg cgaaaacgct tgattaccaa agcccgttga ccaccacgcg ctatacgctg
     9361 ccggagctgt tagacaattc ccagcagttg atccatgaag cgcggaaaat gacgcctccg
     9421 cagattagca cgctgatgcg catcagcgac aaactggcgg gtatcaacgc cgctcgcttt
     9481 catgactggc agccagattt cacgccggag aatgcccgcc aggcgattct ggcgtttaaa
     9541 ggtgatgtct acaccggctt gcaggccgaa accttcagcg aagacgattt cgattttgcc
     9601 caacagcatt tgcgaatgct ttccggcttg tatggcgtac tccgcccgct cgatttaatg
     9661 cagccttatc gtctggaaat ggggatccgt cttgagaatg cccgagggaa agatctgtat
     9721 caattctggg gagatatcat caccaacaag ctgaacgagg cgctcgcagc acaaggcgat
     9781 aatgtggtga ttaatctggc gtcagatgag tattttaaat cggtgaagcc gaagaaattg
     9841 aatgccgaga ttatcaagcc ggtgttcctc gatgagaaga acggcaaatt taagatcatc
     9901 agcttctacg ctaagaaagc acgcggtctg atgagtcgtt ttattattga aaatcggctg
     9961 accaaaccag agcaactgac tggttttaat agcgaaggtt acttctttga tgaagattcc
    10021 tccagcaatg gcgaactggt gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc
    10081 cggatgcggc gtgaacgcct tatccggcct acaaaagcat gcagattcaa tatattgcag
    10141 aattcacgta gatcctgata agcgtagcgc atcaggcgat tttgcgtttg tcatcagact
    10201 taatccgggc atgatagccc ggatttccat caagattagc gacgaacagc gatcgcttcg
    10261 atctcaattt ttacgtcttt cggcagacgg gcaacttcaa cgcaagaacg tgccgggaag
    10321 gtggcgttgt gttcggtgaa gaaggcttcg taagtggcgt ttacggttgc gaagtcgttc
    10381 agatctttca caaacacggt ggttttaacg atatcgccca ctttcaggcc agcggcttcg
    10441 acaatcgctt ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc tgccggtact
    10501 tcgcccgttt tcggatttac cgggatctgg ccggaggtga tgatcatatt gcccagatca
    10561 acgccctgta cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc
    10621 atgatttctc ctttattaca gcggtaaaaa gtctggctca ttatagggag ccagactttt
    10681 attaccaacc gcaattaatt ggccagcacc acattatggg aaaactcttt ttcacagtat
    10741 ttgcatttga gcgcgatgtc attggcgcgt tttcgcacgg caaagctgga agaaaccggt
    10801 tcggcatggc tgatacagtt gctgttcggg cagaccagca cattgtcgat gcgctctggc
    10861 agactggggc gcgatttacc caccacttca tagttgtcga tacggttaac cgtggcttgc
    10921 ggcgcataca atgccagttg atctacctga tcttcactca aaaaggtatt ttcgattttg
    10981 atcagatctt tgcggcccat ctcgccagaa ggcaagttca gaccgatggt gatgcgctga
    11041 tccgtttcgg tcagcttgaa cagactcaac agcttaaaac cgatctgggc ggggatatgg
    11101 tcaattaccg tgccgcgttt aatagcttca acctgcaatt tattatcgtg tgtcatctct
    11161 atttcccctt acagtaccag atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg
    11221 aaaatcccgt tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc aacatccgtc
    11281 gcaatctcat caacacgcgg cagcggatgc agcactttca tattggcttt ggcgttgtgg
    11341 agatcgctgg cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga cgggtccaga
    11401 cgctcttttt gcacgcgggt catgtacagg atgtctactt ccgccatcac ttcttcaata
    11461 gagctgtgca gactccatgc gatccctttt tcatcgagca tatccagaat gtattgcggc
    11521 attgccagcg cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc
    11581 tgagtcaggg agtgaacggt gcggccatat ttcaggtcac caaccattgc gacgtggaga
    11641 ttgtccagac gcccctgggt ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga
    11701 tgttggttgg agccatcacc ggcattcagt accggtacat tgccggaaaa ctcggtggcc
    11761 aggcgcgccg caccttcctg cggatgacgc atcactatcg catcgacgta agtgctgata
    11821 accgaaatgg tatcggccag cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg
    11881 gagaagccca ccacgctggc acccaggcgg tgcatagatg tttcgaaaga gaggcgggta
    11941 cgggtagagg cttcgaagaa acagctggca atgactttgt gcttcaacag ctctggttgc
    12001 gggtttgctt tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc gcgactaagg
    12061 tcgtttatgg aaatgatatg tttctgatat agcggattag ccatctttta tctcctgacg
    12121 cctgggcaaa aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa
    12181 aacggcaggc cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac acactgaacc
    12241 atacatcctc ccggcaaatt gtccggcatt atactcatcg tcagaagcgg tgcaagcatt
    12301 tgatgcaata ttttgtcagc gcaaacggtt tatttgaatt aaaagtcaag gtatatgcat
    12361 ttttatttga tgtgattctg caggggaact gtccttcggt atcaataatt gcagacattc
    12421 cctgcttttt cctttttcac tttcacgcaa tcagatatgc attttattcc attcatccga
    12481 cttatagggc ggagtgtgaa agcgaacggc taacactatt gcttactgct cagggatgcg
    12541 cgctatcact ttaatttcaa aatcaaagcc tgccagccat gtaacaccca ccgccgtcca
    12601 gtttggataa ggtggggcgc taaatatttc atttttcacc gtcatgatgt cttcaaattg
    12661 gttttctgga tcggtatgga agctcgtaac atcaatgata tcgtcaaaag tgcatcccgc
    12721 agctgccagg gtcgcatgca aattatcaaa tgccagtctg acttgttgct gaaaatcggg
    12781 ttctggtgtt ccgtcctctc gacttcctac ttgcccggaa acaaacagca aatcgccgga
    12841 acgaatagcc gcagaataac gatgctcagc atacagtgaa tgtcggccag cagggaaaac
    12901 agcggttctt tctaccattt ggttatcctc aagatttacg acatgaacag aagatttctc
    12961 tttaccggga gccgctttta gcggacgacg tgagtaaaca aaacccagac atcatggata
    13021 atggctgggc ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca atggtatgca
    13081 tcgcgccggt aacaaaactg gcttctggcc ctgctaacca tgcgaccata ccagcgacct
    13141 cttccggttg cccatgtctt ttgatagcca taaaactatg caacatatcg cgcattggcc
    13201 cgttggcggg attagcgtcg gtatcaattg gccctggctg gacgacgtta atggtgatcc
    13261 cacgcggtcc gaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg
    13321 cggcataagc agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta
    13381 agatgcgccc gccttcgggc atctgccggg cggcttcaac agaggcatga taaggagcat
    13441 gaatattgat tttgaaaagg cgatcaatat cgtcggcatt caattccagg gcatcgccaa
    13501 agacgccaat acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat
    13561 caatgacagc gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcttgtg
    13621 ccaggtgttc agcggcatct ttcgaccccg cataggtgaa tcgtacattg gccccatcgg
    13681 tgacgaaacg acgtacgata gcggcaccga taccacgact gccaccgagg atgagaactg
    13741 tcttacctgt aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa
    13801 actgttttaa ctttctgtca acaagttttg taatggtcac taaaaaacaa tctcgcgttc
    13861 caggtcgtcc cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc
    13921 accaaaaagg tttcgatgct gtcagtgttg ctgaagttac tgattatctt ggtattaacc
    13981 ccccgagcct ctacgcggct tttggcagta aagctgggtt atttagccgt gtactcaatg
    14041 aatacgtcgg tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccagtag
    14101 gcgagtgcct ggttgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct
    14161 gcgctggctg tatggttctt gaaggtattc atagtcatga tccactagcg cgtgatattg
    14221 ccgttcaata ttatcacgcc gcagaaacga ccatttatga ctatatcgcc aggcggcatc
    14281 cacaaagcgc acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga
    14341 aggcacggga ggggcactca atcgaacaac tctgtgcaac agctgcactg gcgggggaag
    14401 cgataaaaac tcttctcaag gagtgatgct ggccttgatc cgaaaggcgg gaacgcgcct
    14461 gccgataagt tgtgataaga caataattca cgcgttaagg ctggcggaat tgatcatctt
    14521 ttcgtataac gatagaaatg aaacattgtt ttaattaagg agtggaaaag atgatcatcg
    14581 gaaatattca taatcttcag ccgtggctac cccaggagtt acgccaggcg attgagcata
    14641 tcaaagcgca cgttacggca gaaacgccaa agggtaagca cgatatcgaa ggcaatcgtc
    14701 tgttttatct tatctcggaa gatatgaccg agccgtacga agctcgccgt gcggagtatc
    14761 atgcccgcta tctcgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca
    14821 cgcaacctgc aggcacgccg gataccgact ggttagctga taaagacatc gcatttttgc
    14881 cggaaggcgt tgatgagaaa acagttatcc ttaatgaagg tgattttgtt gtgttttatc
    14941 cgggggaagt gcataaaccg ctgtgcgcag tgggcgcgcc agcccaggtt cgcaaagcag
    15001 tagtgaagat gctgatggcg tgatgacttt tcgccgtaaa taactccagg tttacggcga
    15061 gtttgtgaaa agagcgtttt ttgatatttt tttgtgagta gaatttgtaa tgcttagacg
    15121 ttcttattaa ctcaaggagt ccgtcatgag caaaatatca ggttggaatt tttctcaaaa
    15181 cattacatca gccgacaatt gtaaacaaaa aaatgaagac ttagatacct ggtatgtggg
    15241 aatgaatgat tttgcccgaa ttgccggagg gcagaatagc agaagcaata ttctttctcc
    15301 cagagcattt ttggagtttt tggctaagat atttaccctg ggttatgtgg attttagcaa
    15361 acgctccaac gaagcgggta gaaatatgat ggctcatatt aagtcctcat cttatatcaa
    15421 aaataatgat ggtagtgaga taatgaagtt tgttatgaat aatcctgaag gggagcgagc
    15481 ggatttatct aaggtggaaa tagaaattac acttgcctca gcttttaata atgggattcg
    15541 tgaaggacat acagtcatta tatttccaca acctgatggt tcgactaatc gttatgaagg
    15601 gaagtccttt gaaagaaaag atgagagttc attacacctg attactaaca agattctggc
    15661 gtgttaccaa cgcgaagcta acaaggaaat agctcgtcta ttaaatattc cgcaggagtt
    15721 aaataattct caggacttga ataactcgca ggtgagttgt aaagattcag ttgattctac
    15781 gattacggat ttattagaaa aaccattgaa taatgcatta ttagcaataa ggaaagaaca
    15841 tctgctatta atgccttatg tatgtaatga atcgatttca tacttgcttg gtgaaaaagg
    15901 gatactgaaa gaaatagatg gccttaacgc agtaaataat tatcttctta ataataagaa
    15961 agctactgac aatgaaatta atgacatcaa agttaacctg tctcatattc ttattgattc
    16021 cttagatgat gcaaaagtta accttacacc ggtcatcgat tcgattctgg agactttttt
    16081 aaaattccca tatattaatg atgtaagaat actggattgg tgttttaata aaagaatgca
    16141 atatttt
//
LOCUS       DAZFFI010000099        15443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFFI010000099 DAZFFI010000000
VERSION     DAZFFI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.099"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..271
                     /locus_tag="ACHJ12_003760"
     CDS             <1..271
                     /locus_tag="ACHJ12_003760"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4991486.1"
                     /translation="QTEQFQNQKRQYITGAKVRRVNADNLSKILIPVPSMEIQERIVS
                     ILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            393..3509
                     /locus_tag="ACHJ12_003761"
     CDS             393..3509
                     /locus_tag="ACHJ12_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO4991487.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     RNQGYEFISVKSQLAMLSNVREQLQSLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQAGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDISQTLLVMRPYQ
                     IAATERILWKINSSYKAKNWSTPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRVATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYNATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKEEHYVDDEKFAELQTIRLPAERKIQDYRSAYNDIRDW
                     QRREKEAGKKEKLTTDWDDVVFEIDLLKSQEINLDYILGLIFEHNRQNKGKGEMTEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKIVAFIEK
                     FKGVGGQI"
     gene            complement(3734..3818)
                     /locus_tag="ACHJ12_003762"
     tRNA            complement(3734..3818)
                     /locus_tag="ACHJ12_003762"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3782..3784),aa:Leu,seq:caa)
     gene            4014..5033
                     /gene="ahr"
                     /locus_tag="ACHJ12_003763"
     CDS             4014..5033
                     /gene="ahr"
                     /locus_tag="ACHJ12_003763"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4991488.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            5163..6665
                     /gene="yjgR"
                     /locus_tag="ACHJ12_003764"
     CDS             5163..6665
                     /gene="yjgR"
                     /locus_tag="ACHJ12_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4991489.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTIGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(6784..7866)
                     /gene="lptG"
                     /locus_tag="ACHJ12_003765"
     CDS             complement(6784..7866)
                     /gene="lptG"
                     /locus_tag="ACHJ12_003765"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4991490.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7866..8966)
                     /gene="lptF"
                     /locus_tag="ACHJ12_003766"
     CDS             complement(7866..8966)
                     /gene="lptF"
                     /locus_tag="ACHJ12_003766"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4991491.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(9029..9079)
                     /gene="ytgA"
                     /locus_tag="ACHJ12_003767"
     CDS             complement(9029..9079)
                     /gene="ytgA"
                     /locus_tag="ACHJ12_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4991492.1"
                     /translation="MSIIILATISTTQENK"
     gene            9233..10744
                     /gene="pepA"
                     /locus_tag="ACHJ12_003768"
     CDS             9233..10744
                     /gene="pepA"
                     /locus_tag="ACHJ12_003768"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4991493.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10878..11321
                     /gene="holC"
                     /locus_tag="ACHJ12_003769"
     CDS             10878..11321
                     /gene="holC"
                     /locus_tag="ACHJ12_003769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4991494.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11321..14176
                     /gene="valS"
                     /locus_tag="ACHJ12_003770"
     CDS             11321..14176
                     /gene="valS"
                     /locus_tag="ACHJ12_003770"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4991495.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNSGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(14232..15428)
                     /gene="yjgN"
                     /locus_tag="ACHJ12_003771"
     CDS             complement(14232..15428)
                     /gene="yjgN"
                     /locus_tag="ACHJ12_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO4991496.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         4168 a         3814 c         3931 g         3530 t
ORIGIN      
        1 tcaaacagaa caattccaaa atcaaaaaag acagtatatt actggcgcaa aagttcgacg
       61 tgtaaatgct gataatttat ctaagatatt aattccagtc ccttctatgg agatacagga
      121 acgtatagta tctattctcg ataaattcga caccctgaca aactccatca ccgaaggcct
      181 tccgcgtgaa atcgagttgc gccagaaaca atacgagtac tatcgtgatt tactgttcag
      241 tttcccgaaa cctgaaactg tcagtaatta attgaccatt gctaccgacc gggccacctt
      301 aacacccggt ctgtatatag actatttttt acgcgccgga agtcactctt aacgcccttc
      361 cggcccttgc caggcggcac aaaggatgcg ctatgactca tcagacacac accattgctg
      421 aatccaataa ctttatcgtc cttgataagt acatcaaagc tgagcaaaca ggcgacagct
      481 accagagcga atcggacctg gaacgggaac tgattcagga cctgcggaat cagggctatg
      541 aatttatttc ggtaaaatca cagttggcga tgctgtccaa tgttcgggaa cagcttcaga
      601 gcctcaatgg tgtggtattt aatgacagcg agtggcggcg tttcacggag caatatctgg
      661 acaacccgag tgatggcatt ctggataaga cccgtaaaat ccatatcgac tatatttgcg
      721 actttatttt tgatgacgag cgtcttgaga acatctattt gatagataaa aagaatctca
      781 tgcgcaataa ggtgcagatt atccagcagt ttgaacaggc gggttctcat gctaaccgtt
      841 atgacgtcac gatcctggtt aatggcttac cactggtaca aatcgaactg aaaaagcgcg
      901 gcgtggcgat tcgtgaggct ttcaaccaga tacatcgtta cagtaaagag agttttaaca
      961 gcgaaaattc cctgtttaag tatctgcaac tgtttgtcat ttctaacggc accgataccc
     1021 gttactttgc caacaccaca aagcgcgata aaaacagttt tgacttcacc atgaactggg
     1081 cgaaatcaga caacacgctg attaaagacc tcaaagactt taccgctacc tttttccaga
     1141 aacatactct gcttaatgtc ttagtgaact acagcgtgtt cgatattagc caaacgctac
     1201 tagtgatgcg accgtaccag attgccgcca ctgagcgtat cttatggaaa atcaacagct
     1261 cctataaagc gaaaaactgg tctacccccg aaagcggtgg ctttatctgg cacacaaccg
     1321 gttctggtaa aacactgacc agctttaaag ccgcacgtct ggcaacagaa ctggacttta
     1381 ttgataaagt tttctttgtg gtcgacagga aagatctcga ttaccagacc atgaaggaat
     1441 atcagcgttt ttcgccagat agtgttaacg gctcggaaaa tacagcaggt cttaaacgaa
     1501 atctggataa ggacgataac aaaattatcg tcaccactat tcagaaactt aataacctga
     1561 tgaaagcaga aagcgacctg cctgtataca atcagcaagt ggtgtttata tttgatgaat
     1621 gccaccgcag ccagtttgga gaagcgcaga aaaacctgaa gaagaaattc aaacgctatt
     1681 atcagtttgg ttttaccggt acccctattt tcccggaaaa cgctttaggt tcagaaacga
     1741 ccgccagtgt atttggtcgt gaattgcatt catatgttat taccgatgcg attcgagatg
     1801 aaaaagtgct taaattcaag gtggactaca acgatgtgcg gccacagttt aagtctttag
     1861 agacagaaac cgacgagaaa aaactgagtg cggctgaaaa tcagcaggcg tttcttcatc
     1921 ccatgcgtat acaggaaatt acgcaatata ttctgaataa cttccgccag aagacccacc
     1981 gtactttccc aggttccaaa ggctttaatg ccatgctggc agtgagtagc gtggatgccg
     2041 cgaaagccta ttacgcgacg tttaaacggt tacaagagga agcagctaat aaatcggcta
     2101 cctataaacc gctgcgtgtt gcgacaatct tctcctttgc cgccaatgaa gaacaaaatg
     2161 ccattggtga aatttccgat gaaacttttg ataccagcgc aatggacagc agtgctaaag
     2221 agtttcttga cgctgcaatt cgtgagtata acagctattt taaaactaac ttcagcaccg
     2281 acagtaacgg ttttcagaac tactatcgtg atttagccca acgggttaaa aatcaggata
     2341 tcgatctgtt aattgtcgtt gggatgtttt taaccggctt cgacgctcca acattgaaca
     2401 cgctattcgt cgataaaaac ttgcgttttc acggcctgat gcaggcattc tcccgcacca
     2461 accgcattta taacgccact aaaaccttcg gtaacatcgt cactttccgg gatctggaac
     2521 gctcaaccat tgatgccata acgctgtttg gtgataaaaa taccaaaaat gtagtgttag
     2581 aaaagagtta tgcagagtat atggaaggct ttactgatgc tgccactggt gaagctaaac
     2641 gcggctttat ggcagtagtt tcagaactgg aacaacggtt ccctgaccct gccagtattg
     2701 aaagtgaaaa agagaagaaa gacttcgtta aactgtttgg tgaatacctg cgtgccgaga
     2761 acatcctgca aaactatgat gaatttgcca cgctgaaagc cctgcaacaa atcgatctta
     2821 gcgatcctgt tgcggtagaa aaattcaaag aagaacatta tgtggatgat gaaaagttcg
     2881 ctgaattaca aacgattcgt ctccctgctg aacgcaagat tcaggattat cgttctgcct
     2941 ataacgatat tcgtgactgg cagcgccgcg agaaagaggc tggcaaaaaa gaaaagttaa
     3001 ctactgactg ggatgacgtg gtttttgaaa tcgatttgct gaagtctcag gaaataaacc
     3061 tggattatat ccttggactg attttcgaac acaacagaca aaacaaaggt aagggcgaaa
     3121 tgaccgaaga ggtcaaacgc ttaattcgtt caagccttgg gaaccgtgcc aaagaaggtc
     3181 tggtggtcga ttttattcag caaacgaacc tggatgattt gccggataaa gccagcatca
     3241 ttgacgcgtt ttttaccttt gctcaacgcg aacagcaacg tgaagcagaa gccttgatca
     3301 aagaagaaaa tctcaatgaa gaggcggcga aacgctatat tcgcacgtct ttaaaacgcg
     3361 aatacgccac cgaaaatggc acggaattaa acgagacatt gccaaaactc agtccgttaa
     3421 atccgcaata taagacgaaa aaacagacgg ttttccagaa gatcgtcgcg tttattgaga
     3481 agtttaaagg tgtaggcggg cagatataat gtattaatcc gaacctgatc tggcagatag
     3541 ctgtcagatc aggactgagc taatacaaat taatatcgaa ccgataatac caacgcctgt
     3601 accaacattt ttcgtttgcg atgggttgga attggttagc cctgagagag ttaaaatagc
     3661 gagaaagata ggtaactaac ggatttcaga cacaaaaaaa gccgctcttg agcggctcga
     3721 tttgcatacg gtgtggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat
     3781 tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac
     3841 gttgtggatt atacctgtcg cacgccacca tgcaagcgcc agcttgctcg cacctcgcta
     3901 agtgctgaaa ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca ctccaggcac
     3961 cccgccctgc catgctctac acttcccaaa caacaccaga gaaggaccaa aaaatgtcga
     4021 tgataaaaag ctatgccgca aaagaagcgg gcggcgaact ggaagtttat gagtacgatc
     4081 ccggtgagct gaagccacaa gatgttgaag tgcaggtgga ttactgcggg atctgtcatt
     4141 ccgatctgtc aatgatcgat aacgaatggg gattttcaca atatccgctg gttgccgggc
     4201 atgaggtgat tgggcgcgtg gtggcgctcg ggagcgccgc gcaggataaa ggtttgcagg
     4261 tcggtcagcg tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc gacgcctgta
     4321 ttagcggtaa tcagatcaac tgcgagcaag gtgcggtgcc aacaattatg aatcgcggcg
     4381 gttttgccga gaagttgcgt gcagactggc aatgggtgat tccactgcca gaaaatattg
     4441 atatcgagtc cgccgggccg ctgctgtgtg gtggtatcac ggtctttaaa ccactgttaa
     4501 tgcaccatat cactgccacc agccgcgttg gggtaattgg tattggcggg ctggggcata
     4561 tcgctataaa acttctgcac gcaatgggat gcgaggtgac agcctttagt tctaatccgg
     4621 cgaaagagca ggaagtgctg gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc
     4681 aggcactgaa agcactggcg gggcagtttg atctcattat caacaccgtc aacgtcagcc
     4741 tcgactggca gccttatttt gaggcgctga cctatggcgg taatttccat acggtcggtg
     4801 cggttctcac gccgctgtct gttccggcct ttacgttaat tgcgggcgac cgcagcgtct
     4861 ctggttcagc aaccggcaca ccttatgagc tgcgtaagct gatgcgtttt gccgcccgca
     4921 gcaaggttgc gccgaccacc gaactgttcc cgatgtcgaa aattaacgac gccatccagc
     4981 atgtacgcga tggtaaagcg cgttatcgcg tggttctgaa agccgacttc tgatcccttt
     5041 gccgggtgcc tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca atctgctgaa
     5101 tttagcggtg aagagtgcac agccgttgcc tatactccag gcaggaaact ggaggatatc
     5161 tcatgagcga acccctgtta attgcccgca cgccggacac agaactgttt ttactgccgg
     5221 gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa actgtcacgc
     5281 tgcaaaaact ggcggagtct ctatcggaaa tcggtgttcc ggtgtttatg gccgatgtga
     5341 aaggcgatct gaccggcatc gcgcaggcag gaacggcatc ggaaaaacta ctcgcaaggc
     5401 ttaaaaatat cggcgtcaat gactggcaac cgcatgctaa tccggtggtg gtgtgggata
     5461 tctttggcga gaaaggccat ccggtgcggg cgacggtttc ggatctgggg ccgctgttgc
     5521 tggcgcggct gttgaatctc aacgatgtgc aatctggcgt gctgaatatc atcttccgta
     5581 ttgctgacga tcagggattg ttgctgctcg actttaaaga tctgcgggcg attacccagt
     5641 acatcggcga taacgccaaa tctttccaga atcagtacgg taatatcagt agcgcatcgg
     5701 ttggtgccat ccagcgcgga ctgttgtcgc tggaacagca aggcgcagca cacttctttg
     5761 gcgagccgat gctggatatc aaagactgga tgcgcaccga taccaacggt aaaggcgtta
     5821 tcaatatcct cagcgccgag aagctttatc agatgccgaa actgtacgcc gccagcctgc
     5881 tgtggatgct ttcagagttg tatgaacaat tgccggaagc aggcgatctg gagaaaccaa
     5941 aactggtgtt tttcttcgac gaggcacatc tgctgtttaa cgatgcaccg caggtactgc
     6001 tggataagat tgagcaggtg atacggctta ttcgctcaaa aggcgtaggc gtctggttcg
     6061 tttcgcaaaa cccgtctgat attccggata atgtgctcgg gcagctcggt aatcgcgttc
     6121 aacacgcttt gcgtgctttt acgcccaaag atcagaaagc ggtaaaagct gcggcgcaaa
     6181 ccatgcgggc caatccggca tttgataccg aaaaggcaat ccaggaactg gggaccggcg
     6241 aggcgttaat ctcgtttctt gatgtgaaag gaagtccttc agtggtggaa cgggcgatgg
     6301 tgattgcgcc ttgttcgcgg atgggaccgg tgacggaaga tgagcgtaat ggcttgatta
     6361 atcactctcc ggtgtatggc aagtacgagg atgaggtgga ccgggaatcc gcctatgaga
     6421 tgttgcaaaa aggcgtacag gcaagcgccg agcagcaaaa taatcccccc gcgaaaggga
     6481 aagaggtggc ggtggatgac ggcattcttg gtggattaaa ggatattttg tttggcacta
     6541 tcggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggcgaaa agcgccgccc
     6601 gccaggtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg ggaagaagaa
     6661 ggtaagtcag gtgcggtctg tgcccctcac cctaaccctc tccccaaagg ggcgagggga
     6721 ccgatcgaac tcgtatttga ctttatcaac aatgtgaaag ggagctttcg ctcccttgct
     6781 tggttacgat tttctcatta acagccacag gctgattaag aagaagctgg cgcttggcaa
     6841 cagtgcgccg atgatcggcg ggatgccata aaccaacgtc aacgggccga agatctggtc
     6901 cagtacgtag aagacaaaac cgaagctgat accggtgacc acacgcacgc ccatcggtac
     6961 gctacgcagt gggccaaaga tgaacgacag cgccatcagc atcatcaccg ccacggatag
     7021 cggctggaag attttgctcc acatgttgag ctgataacgt ccagcgtcct gaccgctcga
     7081 cttcagatac ttcacgtagt tgtgcaaacc gctgattgag agtgcatccg ggtccagcgc
     7141 caccacgccc agtttgtctg gcgtgaggtt ggttttccag gtgccgctca ccgtctgcga
     7201 accggtgatc tgtttcggat tggtcagatc agattcatca acctgcgaca gacgccagac
     7261 tttatgttcc gggtcaaact tcgcagtagc ggcatagcgt acggattgca gacgacgatt
     7321 ttcgttaaag gcataaatgc tgatgccacc taactcttcg tcacctttaa cccgctcaat
     7381 gtagacgaag ttgttgccat ctttcgccca taagccttgc tgggtagaga gcaacgagcc
     7441 gccgtacatc gcctgcgcac ggtagttacg cgccatctgc tcgccctgcg gcgcgaccca
     7501 ttcaccaatc gccatcgtca gcaagactag cggaatggcg gttttcatca ccgacagcgc
     7561 aacctgcata cgggtgaaac cagaagcctg catcaccacc agttcgctgc gctgcgccag
     7621 catcccaaga ccaagcaacg ccccaagcag agctgccatc gggaagaaga tttgcacatc
     7681 tttcggcacg ctcagcaagg tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc
     7741 ggcttttttc agctgatcga caaacttgat aatgcccgac agcgacacca gcatgaacag
     7801 cgtcatcatg atggtggtga aaatagtttt accgatatag cggtcaagta cgccaaaagg
     7861 ttgcatcaca ccgctccttt acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg
     7921 tcccaaaggt tgagaacaat cgccaaagcc agataaatca ggttaacggt ccacatccac
     7981 agcgtcgggt ccagcttacc tttaccgccg ttcgatttca gggaggtctg gatcaggaag
     8041 aaaagtagat acagcagcat ggctggcagc atcgacagta cgcgtccctg acgtgggtta
     8101 accacgctca gcggtacgac cataagtgcc atcataaaca cggtgaatac caacgtgata
     8161 cgccagttca gttctgcgcg agcacgatcg gtgtcagtgt tccacaatgt gcgcatgtcc
     8221 atctggtcgg tatcgttcgg gtcgagcgcc accgcctggt gaccaatgat cgcctgataa
     8281 tcctggaaat ccgtaatgcg gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc
     8341 tggttgagag tgacgacctg ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc
     8401 accaccacag aaggacgtgc attacctttt ggtcgaattt gcgcgaggaa cacatctttg
     8461 aaatcgctgc cgtcaacgct ttcgatgaac agcaccgagc tgccattagt cgcttgctgg
     8521 aattgccctt gcgccagcgc cgccatgcca gggttcgctt tcgcttctgc taacacttca
     8581 tcctgatgac gcgatgacca cggtcccgcc cacatcacgt taaccgccgc gacgattgcc
     8641 gtgaataccg caaggatcat tgccgctttc accagaaccg ctttgctcag gccgcaggca
     8701 tgcattaccg taatttcact ttcggtatac agtttgccca gcgtcatcag cagcccgagg
     8761 aacaggctta atggcaggat aagctgcgcc atttccggca cgcccaaccc gagaagggag
     8821 agcaccagat tcgccggaat atcgccgtca accgctgcgc cgaggatcct cactaacttt
     8881 tgacagaaga agatcaaaag caagatgaag agtatcgcca gctggctttt gagcgtctcc
     8941 cgcaccagat atcttatgat tatcacttta aatacgcccg taaaaactcg tcttttgcag
     9001 gattttagct tgtttcatgg cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg
     9061 ctaagataat tatactcaac ggattcacct ctcagatttt gttctgacgt gccaatgccg
     9121 taataacgtt aagattaaca cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg
     9181 caattctatc tgtagccacc gccgttgtct ttaagattca ggagcgtagt gcatggagtt
     9241 tagtgtaaaa agcggtagcc cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt
     9301 cgaaccacgt cgcctttctc cgattgcaga acagctcgat aaaatcagcg atgggtacat
     9361 cagcgccctg ctacgtcggg gcgaactgga aggaaaaccg gggcagacat tgttgctgca
     9421 ccatgttccg aatgtacttt ccgagcgaat tctccttatt ggttgcggca aagaacgtga
     9481 gctggatgag cgtcagtaca agcaggttat tcagaaaacc attaatacgc tgaatgatac
     9541 tggctcaatg gaagcggtct gctttctgac tgaactgcac gttaaaggcc gtaacaacta
     9601 ctggaaagtg cgtcaggctg tcgagacggc aaaagagacg ctctacagtt tcgatcagct
     9661 gaaaacgaac aagagcgaac cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac
     9721 ccgccgtgaa ctgaccagcg gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg
     9781 gattaaagca gcaaaagatc tcggcaatat gccgccgaat atctgtaacg ccgcttacct
     9841 cgcttcacaa gcgcgccagc tggctgacag ctacagcaag aatgtcatca cccgcgttat
     9901 cggcgaacag cagatgaaag agctggggat gcattcctat ctggcggtcg gtcagggttc
     9961 gcaaaacgaa tcgctgatgt cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg
    10021 cccaatcgtg ctggtgggta aaggtttaac cttcgactcc ggcggtatct cgatcaagcc
    10081 ttcagaaggc atggatgaga tgaagtacga tatgtgcggt gcggcagcgg tttacggcgt
    10141 gatgcggatg gtcgcggagc tacaactgcc gattaacgtt atcggcgtgt tggcaggctg
    10201 cgaaaacatg cctggcggac gagcctatcg tccgggcgat gtgttaacca ccatgtctgg
    10261 tcaaaccgtt gaagtgctca acaccgacgc tgaaggccgc ctggtactgt gcgacgtgtt
    10321 aacttacgtt gagcgttttg agccggaagc ggtgattgac gtggcgacgc tgaccggtgc
    10381 ctgcgtgatc gcgctgggtc atcacattac tggtctgatg gcgaaccaca atccgctggc
    10441 ccatgaactg attgccgcgt ctgaacaatc cggtgaccgc gcatggcgct taccgctggg
    10501 tgacgagtat caggaacaac tggagtccaa ttttgccgat atggcgaaca ttggcggtcg
    10561 tcctggtggg gcgattaccg caggttgctt cctgtcacgc tttacccgta agtacaactg
    10621 ggcgcacctg gatatcgccg gtaccgcctg gcgttctggt aaagcgaaag gcgcaaccgg
    10681 tcgtccggta gcgttgctgg cacagttcct gttaaaccgc gccgggttta acggcgaaga
    10741 gtaagtttgc gttttaccct caccctaacc ctctccctaa gggagaggga accgatcgag
    10801 cgcaatagaa attcattgct catcttttag ggtgaggaat gcagcaatat ttaaatccac
    10861 cacaagaagc cccatttatg aaaaacgcga cgttctacct tctggacaat gacaccaccg
    10921 tcgatggctt aagcgccgtt gagcagctgg tgtgtgaaat tgccgcagaa cgttggcgca
    10981 gtggtaagcg cgtgctcatc gcctgtgaag atgaaaagca ggcttaccgg ctggatgaag
    11041 ccctgtgggc gcgtccggca gaaagctttg ttccgcataa tttagcggga gaaggaccgc
    11101 gcggcggtgc gccggtggag atcgcctggc cgcaaaaacg cagcagcagc ccgcgcgata
    11161 tattgattag tctgcgaaca agctttgcag attttgccac cgctttcaca gaagtggtag
    11221 acttcgttcc ttatgaagat tctctgaaac aactggcgcg cgaacgctat aaagcctacc
    11281 gcgtggctgg tttcaacctg aatacggcaa cctggaaata atggaaaaga catataaccc
    11341 acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact ttaagcctaa
    11401 tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg tcaccggcag
    11461 tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc gctatcagcg
    11521 catgcagggc aaaaacaccc tgtggcaggt cggtactgac cacgcaggga tcgctaccca
    11581 gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa acccgtcacg actacggccg
    11641 cgaagctttc atcgacaaaa tctgggaatg gaaagcggaa tccggcggca ccattacccg
    11701 tcagatgcgc cgtctcggca actccgtgga ctgggagcgt gaacgcttca ccatggacga
    11761 aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag acctgattta
    11821 ccgtggcaaa cgcctggtaa actgggatcc gaaactgcgc accgctatct ctgacctgga
    11881 agtggaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc tggctgacgg
    11941 cgcgaaaacc gcagatggta aagattatct ggtggttgcg actactcgtc cggaaaccct
    12001 gctgggcgat accggcgtgg ccgttaaccc ggaagatccg cgttacaaag atctgattgg
    12061 caaatatgtc attctgccgc tggttaaccg tcgtattccg atcgttggcg acgaacacgc
    12121 cgacatggaa aaaggcaccg gctgcgtgaa gatcaccccg gcgcacgact ttaacgacta
    12181 tgaagtcggt aaacgtcacg ccctgccgat gatcaacatc ctgacctttg acggcgatat
    12241 ccgtgaaagt gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt attccagcga
    12301 gatccccgca gaattccaga aactggagcg ttttgctgca cgtaaagcgg tcgttgctgc
    12361 ggttgacgca ctcggcctgc tggaagaaat taaaccacat gacctgaccg ttccttatgg
    12421 cgaccgtggt ggcgtggtca tcgaaccgat gctgaccgac cagtggtacg tgcgtgccga
    12481 tgtcctggcg aaaccggcag ttgaagcggt tgagaacggc gacattcagt tcgtaccgaa
    12541 gcagtacgaa aacatgtact tctcctggat gcgcgatatt caggactggt gtatctctcg
    12601 tcagttgtgg tggggtcacc gtatcccggc atggtatgac gaagcgggta acgtttatgt
    12661 tggccgcaac gaagacgaag tgcgtaaaga aaataacctc ggtgctgatg ttgtcctgcg
    12721 tcaggacgaa gacgttctcg acacctggtt ctcttctgcg ctgtggacct tctctaccct
    12781 tggctggccg gaaaataccg acgccctgcg tcagttccac ccaaccagcg tgatggtatc
    12841 tggtttcgac atcatcttct tctggattgc ccgcatgatc atgatgacca tgcacttcat
    12901 caaagatgaa aatggcaaac cgcaggtgcc gttccacact gtttacatga ccggcctgat
    12961 tcgtgacgac gaaggccaga agatgtccaa atccaagggt aacgttattg acccactgga
    13021 tatggttgac ggtatttcgc tgccagaact gctggaaaaa cgcaccggca atatgatgca
    13081 gccacagctg gcggacaaaa tccgtaagcg caccgagaag cagttcccga acggtattga
    13141 accgcacggt actgacgcgc tgcgcttcac cctggcggcg ctggcttcta ccggtcgcga
    13201 tatcaactgg gatatgaaac gcctggaagg ttaccgtaac ttctgtaaca agctgtggaa
    13261 cgccagccgc tttgtgctga tgaacacaga aggtcaggat tgcggcttca acagcggcga
    13321 aatgacgctg tcgctggcgg atcgctggat tctggcggag ttcaaccaga ccatcaaagc
    13381 gtaccgcgaa gcgctggaca gcttccgctt cgatatcgcc gcaggcattc tgtatgagtt
    13441 cacctggaac cagttctgtg actggtatct ggaactgact aagccggtaa tgaacggtgg
    13501 caccgaagcc gaactgcgcg gtactcgcca tacgctggtg accgtgctgg aaggtctgct
    13561 gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc gtgtgaaagt
    13621 actttgcggt atcactgccg acaccatcat gctgcagccg ttcccgcagt acgatgcatc
    13681 tcaggttgat gaagccgcac tggccgacac agagtggctg aagcaggcga tcgttgcggt
    13741 acgtaacatc cgtgcagaaa tgaacatcgc gccgggcaaa ccgctggagc tgctactgcg
    13801 tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac cgtggcttcc tgcaaacgct
    13861 ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac aaaggtccgg tttccgttac
    13921 taagatcatc gacggcgcag agctgctgat cccgatggct ggcctcatca acaaagaaga
    13981 tgagctggcg cgtctggcga aagaagtggc gaagatcgaa ggcgaaatca gccgtatcga
    14041 gaacaagctg gcgaatgaag gctttgtcgc ccgcgcaccg gaagcggtca tcgcgaaaga
    14101 gcgtgaaaag ctggaaggct atgcagaagc gaaagcgaag ctgattgaac agcaggctgt
    14161 tatcgccgcg ctgtaatcaa aacacagtaa tgaaaacgaa ggccggagca tgctccggcc
    14221 ttttttatct cttacagaaa tggtaaagaa ggcataattc cccgtgaaat cctggctaag
    14281 aagcctttat ctggttgttc ttctttattg attaaaggaa ggtcatccaa atcgcccagc
    14341 aaatacgtat tttttgcctg ccagtcaatc atccatattt tcagcagtgg ataagccaga
    14401 ccgcccgtaa tcccggatat caccaccaac gcacacatgc gataaagcat accgtggtac
    14461 gttaaagttg agcgaaaacg aatacgccca tcattcagtg acaggttgct cataaaatga
    14521 tttcgcaacg acaccgttaa ataactggtg ctaacagcaa tcccaaaata atagattaac
    14581 tgcgcgatta tcattttacg ttgcatttcc ataaattgct gtaaattctc aatatcatca
    14641 tttgcataca cagaactatc atacgcattt aatatttgat cgaagataat ataaccagca
    14701 acagcgagaa aaggtaataa agctaaaaat gcgagaatgg catattttat acaatacgta
    14761 gtatctaatt tcaccttgaa acgatgtata ccgaaactgg tattgctcca gagaaaactc
    14821 attaccaggc tatataaagt accattaaaa ataccaatgg aaacaatacc aactattgcc
    14881 atcagaacca cagatataat aacactactt gaactattgg catgtagcat ctttgtcgag
    14941 ataaagaaaa cagtccccat cccaatggcc attaaaatcg gcaagaaaaa ggttacccac
    15001 cagaaccctt tcatagagca attaaaacta aatcttacgc cgttgagact ggtcatcaag
    15061 gcctgataac gcagtccttt tgctgccata aatataagca aaaccaacag taacaaagtc
    15121 aaaacacagc caacaagtgg catatctgct gacactgtca ttaagattgc gaaatagcaa
    15181 aaaacaaaaa caagacaact aacaaaaaca ttcccaccag taattccata agaaaatcgt
    15241 tgtccgttaa cttccatatt agcatagaga taacgcttac atttcattaa tgcccatggc
    15301 aaatagatac ctagcgttat aatcgttaac aacacattca ccacacaaat aagaaaatat
    15361 ctctcacctg taccatgaaa aacaaacgaa tgggacggaa catccatttc attaataact
    15421 tgagccataa aaattccctg aat
//
LOCUS       DAZFFI010000100        15352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFFI010000100 DAZFFI010000000
VERSION     DAZFFI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.100"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..647
                     /gene="yceJ"
                     /locus_tag="ACHJ12_003772"
     CDS             81..647
                     /gene="yceJ"
                     /locus_tag="ACHJ12_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4991497.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMAVKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            651..1226
                     /gene="yceI"
                     /locus_tag="ACHJ12_003773"
     CDS             651..1226
                     /gene="yceI"
                     /locus_tag="ACHJ12_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4991498.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1268..2320)
                     /gene="trhO"
                     /locus_tag="ACHJ12_003774"
     CDS             complement(1268..2320)
                     /gene="trhO"
                     /locus_tag="ACHJ12_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4991499.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2545..3465
                     /gene="lpxL"
                     /locus_tag="ACHJ12_003775"
     CDS             2545..3465
                     /gene="lpxL"
                     /locus_tag="ACHJ12_003775"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4991500.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3637..4863
                     /gene="mdtG"
                     /locus_tag="ACHJ12_003776"
     CDS             3637..4863
                     /gene="mdtG"
                     /locus_tag="ACHJ12_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO4991501.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4946..5320
                     /gene="msyB"
                     /locus_tag="ACHJ12_003777"
     CDS             4946..5320
                     /gene="msyB"
                     /locus_tag="ACHJ12_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4991502.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5321..5548)
                     /gene="yceK"
                     /locus_tag="ACHJ12_003778"
     CDS             complement(5321..5548)
                     /gene="yceK"
                     /locus_tag="ACHJ12_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4991503.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPLDIHHGPYE"
     gene            complement(5721..8264)
                     /gene="mdoH"
                     /locus_tag="ACHJ12_003779"
     CDS             complement(5721..8264)
                     /gene="mdoH"
                     /locus_tag="ACHJ12_003779"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4991504.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8257..9792)
                     /gene="mdoG"
                     /locus_tag="ACHJ12_003780"
     CDS             complement(8257..9792)
                     /gene="mdoG"
                     /locus_tag="ACHJ12_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4991505.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10186..11343
                     /gene="mdoC"
                     /locus_tag="ACHJ12_003781"
     CDS             10186..11343
                     /gene="mdoC"
                     /locus_tag="ACHJ12_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4991506.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11351..12832)
                     /gene="clsC"
                     /locus_tag="ACHJ12_003782"
     CDS             complement(11351..12832)
                     /gene="clsC"
                     /locus_tag="ACHJ12_003782"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4991507.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12774..13307)
                     /gene="ymdB"
                     /locus_tag="ACHJ12_003783"
     CDS             complement(12774..13307)
                     /gene="ymdB"
                     /locus_tag="ACHJ12_003783"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4991508.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13402..13712)
                     /gene="ymdA"
                     /locus_tag="ACHJ12_003784"
                     /pseudo
     CDS             complement(13402..13712)
                     /gene="ymdA"
                     /locus_tag="ACHJ12_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
     gene            complement(13833..14165)
                     /gene="csgC"
                     /locus_tag="ACHJ12_003785"
     CDS             complement(13833..14165)
                     /gene="csgC"
                     /locus_tag="ACHJ12_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4991509.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14224..14679)
                     /gene="csgA"
                     /locus_tag="ACHJ12_003786"
     CDS             complement(14224..14679)
                     /gene="csgA"
                     /locus_tag="ACHJ12_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4991510.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14720..15175)
                     /gene="csgB"
                     /locus_tag="ACHJ12_003787"
     CDS             complement(14720..15175)
                     /gene="csgB"
                     /locus_tag="ACHJ12_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4991511.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3816 a         3937 c         3654 g         3945 t
ORIGIN      
        1 tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca
       61 aattcgtatt ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat
      121 ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt
      181 ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa
      241 gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc
      301 caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca aaggctggac
      361 atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg
      421 ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg
      481 ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag
      541 tactgtccgt tatgcacgga tttatggccg tgaagcatca tttcatcgat aaagacgaca
      601 ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa
      661 gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg
      721 attacaaaat tgacaaagaa ggtcagcacg cctttgttaa tttccgcatc cagcaccttg
      781 gctatagctg gttatacggc acctttaaag atttcgacgg tacttttacc tttgacgaaa
      841 aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc
      901 acgccgaacg cgataaacat cttcgcagtg cagatttcct caatacagca aaatatccac
      961 aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg
     1021 atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg
     1081 gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca
     1141 aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt
     1201 cagtggaagg cgtacagcag aagtaagcgt gaaatgttgc cggatggcac gcatccggca
     1261 atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact
     1321 tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg
     1381 gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcgcata
     1441 ctggacactg aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc
     1501 acggcgcgcc gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc
     1561 gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct
     1621 tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt
     1681 tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt
     1741 tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg
     1801 ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt
     1861 cgataaatag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact
     1921 caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc
     1981 gtaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc
     2041 gtaaaccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc
     2101 tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa
     2161 cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt
     2221 ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt
     2281 ttagcgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga
     2341 tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt
     2401 ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca
     2461 gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg
     2521 aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt
     2581 catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt cgtgcaattg
     2641 ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg
     2701 aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa
     2761 caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa
     2821 accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggactga agtgatcggc
     2881 atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat
     2941 tttctgacac tggaactggg cgcgcggcag tttggtatgc aggaaccggg gattggcgtt
     3001 taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca
     3061 aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc
     3121 gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg
     3181 ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc
     3241 ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg
     3301 attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg
     3361 atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac
     3421 cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccctgccgga
     3481 tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct
     3541 ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta
     3601 tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacact
     3661 cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc
     3721 gccttcagtc tggtaatgcc cttcctaccc ctctacgttg agcagcttgg agttaccggt
     3781 cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc
     3841 atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta
     3901 cgctctgccc tcggcatggg catcgtgatg gtgttgatgg gactggcaca aaatatctgg
     3961 cagtttttga tcctacgggc gcttcttggg ttacttggcg gatttgtccc caacgctaat
     4021 gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg tacgctctcc
     4081 acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct cgccgatagc
     4141 tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc
     4201 accctgttct gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg
     4261 cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcaccacg
     4321 ttaatcatcc aggtggcgac aggctcaatt gcccccattc tgacgctgta tgtccgcgaa
     4381 ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc
     4441 gtggcggctc tgctaagtgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa
     4501 aagatcctga ttacggcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt
     4561 cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta
     4621 ctccccgccg tacagacact attagtttat aactcgagca accagatcgc cgggcgtatc
     4681 ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca
     4741 gcgatttcag caaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta
     4801 ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac
     4861 tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc
     4921 tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga
     4981 cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag acgcgaatgt
     5041 gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga
     5101 gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg gtgaagccgc
     5161 gcaaagtgtt tttgatggcg actatgatga gatagagatc cgccaggagt ggcaggaaga
     5221 gaatacatta catgaatggg acgaggggga gtttcagctt gagccaccgc tggataccga
     5281 ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac gggccatgat
     5341 gaatgtcgag cggcagcagt aaagtatcaa aaaccaggga gaatggcaga tcaaggattg
     5401 tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa tattggttgc
     5461 catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc agcaaggtca
     5521 ccatgatgct caccacaatt aaacgcattt gatacctctt ccgtaaaaaa ataccggcat
     5581 aacgccggta tttttattgg ctcgttgttt ttgccagatg cgacgtgaac gccttaccca
     5641 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
     5701 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
     5761 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg ggaattccag
     5821 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
     5881 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
     5941 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat
     6001 gacgggttaa acactgcgtg cataaaacca tcatcaaggg agcgttgacg gttcatctca
     6061 aggaaccgat cggtatcaac cagtacctgc ggcggtgaat actcttccgg gatcaggaac
     6121 aatttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
     6181 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac
     6241 cacgccatac caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
     6301 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
     6361 ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
     6421 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
     6481 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
     6541 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     6601 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac ctgcaatgca
     6661 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     6721 aggaataccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     6781 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggtaat
     6841 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     6901 aacgccgctt ctacaaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     6961 agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc
     7021 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     7081 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     7141 gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag cccgcacaaa
     7201 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     7261 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     7321 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     7381 tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc aaagtgtttg
     7441 gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc
     7501 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     7561 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     7621 cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     7681 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     7741 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     7801 taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     7861 gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     7921 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     7981 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt cagctgatcg
     8041 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     8101 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     8161 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     8221 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     8281 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     8341 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     8401 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     8461 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     8521 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     8581 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     8641 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     8701 gacgatgtta tcgttggttt catcgttggt tggaatttct accagctcaa cgctgccttt
     8761 accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac gatcatcgag
     8821 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt gcgggttttc
     8881 catagagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     8941 gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt
     9001 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     9061 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     9121 tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     9181 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     9241 ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa ggccataaac
     9301 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     9361 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     9421 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     9481 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     9541 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     9601 aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct
     9661 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     9721 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     9781 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     9841 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     9901 cgttcagcac gctggctcat ggattaagca tctgattgca accgttttca ctttccgtgc
     9961 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt tatttatcaa
    10021 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
    10081 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
    10141 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
    10201 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
    10261 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccatca
    10321 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
    10381 atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
    10441 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
    10501 caatttatta tgctgcaata tgtcaaagga aaagcggaaa gttggcctgg gctgtcattg
    10561 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
    10621 ttagtagtca tgacgacgct gtgcgtatgg atatttaagc gcatcagaaa taatttagaa
    10681 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
    10741 ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg
    10801 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
    10861 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
    10921 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
    10981 agtggcgatg cctggatgta cgaaaccgag tcagtgatca ccatggtcct cggtctgtgg
    11041 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
    11101 acttattttg ttaacgcatc gctatttatc tatctggttc accacccgtt aacgctgttt
    11161 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
    11221 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
    11281 aagtttttgt tttctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
    11341 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
    11401 gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat
    11461 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
    11521 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
    11581 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
    11641 taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg gttatgccgc
    11701 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat
    11761 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
    11821 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
    11881 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
    11941 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
    12001 cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc
    12061 catcaaccag atgatttata aacggactgg attccatttt gcgtaagtaa cgatgcgtca
    12121 tagcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
    12181 catccagcac ttgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
    12241 cggcaacgtc ctctaccacg ggccctattg ccataacatc taaatccgaa aaaagtggct
    12301 cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc gtcaccacgc
    12361 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
    12421 aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg
    12481 gatggctgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
    12541 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac
    12601 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
    12661 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
    12721 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
    12781 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
    12841 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
    12901 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
    12961 actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc atcctgcaaa
    13021 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg cactacggct
    13081 ttagcgggaa gagcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
    13141 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
    13201 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
    13261 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
    13321 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
    13381 caacgtgcgt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
    13441 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat taccgctcat
    13501 taacgctttg ctggaatagg ttttgcccgg aacagaacca ttctgtgggc agttcatttc
    13561 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
    13621 gatccccccc cttgcgcggt gcttcctgcg atggcaataa aaggaaaaag caaactgccg
    13681 agcataagag agtttaaaaa aggtctgaac atttattcta tttcctcaat gattagtcat
    13741 ccttgagggt tgtgttatcc atactttcag gttggctgat tcgcagcaga ccattctctc
    13801 cagattcatc ttatgctcga tatttcaaca aattaagact tttctgaaga gggcggccat
    13861 tgttgtgata aatgaagtga ctgtccatca gaaacagtaa caactatttt cacccgatct
    13921 tccggggaaa tatttaaact caacttcgtc aaagcaatag gttgattagc aggcaatgag
    13981 agggtctttt cttgcttcgt ctgactttgc cctgaactgc cttcgcgcag ggacaatatt
    14041 tgtactctgc acagacaaga ttgagtaaga gtgacttcag gaataatggt atacacatcc
    14101 ccttgctggg tcgtattaaa ggttatctgg ctggaaagtg ccgcaaggag taataacgca
    14161 ttcatattct tctcccgaaa aaaaacaggg cttgcgccct gtttctgtaa tacaaatgat
    14221 gtattagtac tgatgagcgg tcgcgttgtt accaaagcca acctgagtca cgttgacgga
    14281 ggagttagat gcagtctggt caactgcagc accgttgcca ccaccgaact gtttaacagt
    14341 catttcagaa tttttgccgt tccactgatc aagagtggcg ctgttaccga agccacgttg
    14401 ggtcagatcg attgagctgt catctgagcc ctgacctaca tctgcaccat taccaccgcc
    14461 atgctgggta atagtcaagt cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt
    14521 accgccaccg tactggtaaa tgttcagctc tgaatttggg ccgctattat taccgccacc
    14581 accgtggtta ccgccgccgc cgtactgagg aacaacacct gccagagcgc taccggagaa
    14641 tacgattgct gcaattgctg ctacttttaa aagtttcatg taaaaccccc atcggattga
    14701 tttaaaagtc gaatggaaat taacgttgtg tcacgcgaat agccatttgc gactgtctct
    14761 gcactacaat tgccgttttt tgagtaccat actgtgtaat atttgcttta ttaccagaac
    14821 ctttctggat aatcatcgca gtattaccat aagcaccttg cgaaatactg gcatcgttgg
    14881 cactgcccgc ctgatcaata tatgcaaggt tataatctcc tgtctggtca atctttgccc
    14941 ggttgctact accttcttgc gcaacaaccg ccaaaagttt tgagcctccc tgccgtaact
    15001 gagcactatt attcgtccca gcttgaccaa ttatggctgc ctgattaaat gaagacttac
    15061 tcaattcatt taccgcgaag ttatattctg aattagctaa atcataacct gctgcggctg
    15121 caatcccagg cgcacccagt attgttaaca tcataaataa caatttgttt ttcatgttgt
    15181 caccctggac ctggtcgtac atttaagaaa ttaaatcatt tcaacttggt tgttaacgca
    15241 acctgtattt tgttaacgct gcgttacgat ggaaagtatg tctgcggaaa tatttttaat
    15301 aactcacccg cgcgttgtat ttttcttttt ttgttcatac caaagtatta aa
//
LOCUS       DAZFFI010000101        15097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFFI010000101 DAZFFI010000000
VERSION     DAZFFI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.101"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(141..216)
                     /locus_tag="ACHJ12_003788"
     tRNA            complement(141..216)
                     /locus_tag="ACHJ12_003788"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Phe,seq:gaa)
     gene            complement(322..1029)
                     /gene="yqgA"
                     /locus_tag="ACHJ12_003789"
     CDS             complement(322..1029)
                     /gene="yqgA"
                     /locus_tag="ACHJ12_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4991512.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPAMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1182..1265
                     /gene="yqgH"
                     /locus_tag="ACHJ12_003790"
     CDS             1182..1265
                     /gene="yqgH"
                     /locus_tag="ACHJ12_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO4991513.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            1427..3562
                     /gene="speC"
                     /locus_tag="ACHJ12_003791"
     CDS             1427..3562
                     /gene="speC"
                     /locus_tag="ACHJ12_003791"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4991514.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3612..4868)
                     /gene="nupG"
                     /locus_tag="ACHJ12_003792"
     CDS             complement(3612..4868)
                     /gene="nupG"
                     /locus_tag="ACHJ12_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4991515.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5070..6149)
                     /gene="mltC"
                     /locus_tag="ACHJ12_003793"
     CDS             complement(5070..6149)
                     /gene="mltC"
                     /locus_tag="ACHJ12_003793"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4991516.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGAEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6214..6489)
                     /gene="yggX"
                     /locus_tag="ACHJ12_003794"
     CDS             complement(6214..6489)
                     /gene="yggX"
                     /locus_tag="ACHJ12_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4991517.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6517..7569)
                     /gene="mutY"
                     /locus_tag="ACHJ12_003795"
     CDS             complement(6517..7569)
                     /gene="mutY"
                     /locus_tag="ACHJ12_003795"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4991518.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGTPV"
     gene            7730..8449
                     /gene="trmB"
                     /locus_tag="ACHJ12_003796"
     CDS             7730..8449
                     /gene="trmB"
                     /locus_tag="ACHJ12_003796"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4991519.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8449..8775
                     /gene="yggL"
                     /locus_tag="ACHJ12_003797"
     CDS             8449..8775
                     /gene="yggL"
                     /locus_tag="ACHJ12_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4991520.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8959..9678
                     /gene="yggN"
                     /locus_tag="ACHJ12_003798"
     CDS             8959..9678
                     /gene="yggN"
                     /locus_tag="ACHJ12_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4991521.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9854..10900
                     /gene="ansB"
                     /locus_tag="ACHJ12_003799"
     CDS             9854..10900
                     /gene="ansB"
                     /locus_tag="ACHJ12_003799"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4991522.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11017..12024
                     /gene="yggM"
                     /locus_tag="ACHJ12_003800"
     CDS             11017..12024
                     /gene="yggM"
                     /locus_tag="ACHJ12_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4991523.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12080..13381)
                     /locus_tag="ACHJ12_003801"
     CDS             complement(12080..13381)
                     /locus_tag="ACHJ12_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO4991524.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGIM"
     gene            complement(13381..13884)
                     /locus_tag="ACHJ12_003802"
     CDS             complement(13381..13884)
                     /locus_tag="ACHJ12_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO4991525.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13929..14915)
                     /locus_tag="ACHJ12_003803"
     CDS             complement(13929..14915)
                     /locus_tag="ACHJ12_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO4991526.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4011 a         3613 c         3799 g         3674 t
ORIGIN      
        1 aaaagacacc ctgtttgagg atacgatacc gaactcaatc agtgtaacca caacttacac
       61 aaacggagac cctttgatgc cgcatcctgt attaaccgcc cgtcaaggat ctcatccctt
      121 aaccagcgtg ttctggtata tggtgcccgg aggcggaatc gaaccaccga cacggggatt
      181 ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc
      241 gcctcgtctc gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact
      301 ttgccgctat tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat
      361 tgccagaagg agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg
      421 taatccggtt gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgccgg
      481 tgtggtcagc ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa
      541 tgggatacta atcaccgata ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa
      601 gaaatcaaga aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat
      661 cgccccgaag atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg
      721 aataaaagat tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggt
      781 gactgctgta ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag
      841 cgtagctaaa accatcgctg gaaggttggc acatttcacc accagtaaaa taccaatccc
      901 cagcgatgcc agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg
      961 gctgagcagt gcgccgagaa cgccacccag taatactgca ctagcgttga taaaagggcc
     1021 gatgaccaca agagttcctg ttaactgtat tgtttgattt tcttattatg accgtttggt
     1081 gccgtcgatg gcttgtttgt tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca
     1141 tgattgcgcg aattttctcc tctctgtacg gagtttgccc gatgcacgcc acctccttac
     1201 attctctcgc ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc
     1261 ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta
     1321 ctgatgtctg gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt
     1381 tccaccttgt ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa
     1441 tattgccgcc agtagtgatc tggtatcccg actttcttct catcgtcgcg tggtggcgtt
     1501 gggagatact gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag
     1561 tggcattctt gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga
     1621 acatgctgtt gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg
     1681 gctggagctg gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga
     1741 cacgctgacg cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca
     1801 tggtgcgttt tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa
     1861 cgtctttcgc gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga
     1921 aggatcggcg aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta
     1981 ttttgtgctg aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg
     2041 tggcgatctg gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat
     2101 tcaggcgggg gcgacgccgg tctatctgga agcttcacgt aacccgtttg gtttcattgg
     2161 cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc
     2221 gccagaaaaa gccgacctgc cgcgcccgtt tcgcctggcg attatccagc tgggaaccta
     2281 tgacggcact gtctacaacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta
     2341 cattctgttt gattccgcgt gggtcggtta tgaacaattt atcccgatga tggcggatag
     2401 ctcgccgctg ctattagaac ttaacgaaaa cgatccgggg atctttgtga ctcagtcggt
     2461 gcacaaacag caggcgggat tctcacagac gtcgcagatc cataaaaaag ataaccatat
     2521 ccgcggacag gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc
     2581 ttctaccagc cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga
     2641 aggggagagt gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa
     2701 ggctattctt gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa
     2761 attgtggcag gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga
     2821 gccgggggcg aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc
     2881 gtgcaagctg ttactcacta caccaggtat caatgccgaa accggcgaat atagcgactt
     2941 tggcgttccg gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa
     3001 gtgcgatctc aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc
     3061 acaactggta gagatgttgg cgcagtttga acagcatatt gaggatgact cgccgctggc
     3121 tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg
     3181 ccagttgtgt caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa
     3241 agcgatgttc cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag
     3301 cgcttatatt cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc
     3361 ggcagaaggg gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg
     3421 gggtggggcg gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg
     3481 attttcgccg gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt
     3541 gtacggttat gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt
     3601 tctttgcgta attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat
     3661 ttgaacatcg ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat
     3721 acggtctgcc agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg
     3781 atgccgccga ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg
     3841 gcgcgaattg ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag
     3901 tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc
     3961 gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt
     4021 acgttcttaa taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca
     4081 gagatctgcg aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac
     4141 atcggatctt tgtcgaagct gtgcaggaag gtattaccga acatgttggt aatctgcagt
     4201 tccgcgccca gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc
     4261 gcgaatgcat cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc
     4321 ggaatatgcg gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata
     4381 tacagctgca tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata
     4441 aagccgatgg tgccccagat acggattggc gggaagtcag taacgatatc catcccggca
     4501 ttttgcaggc gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac
     4561 gagttaatca atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac
     4621 agcgtgatag cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg
     4681 tcggccacaa tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa
     4741 actgcgccaa tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc
     4801 gtcagccaac ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta
     4861 agattcatgt taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga
     4921 ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa
     4981 ataattgcaa acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc
     5041 ctgagcatcg tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta
     5101 ttcactttat aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga
     5161 taaacatcgc ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa
     5221 aagactcgca gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc
     5281 gatgttgggt tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg
     5341 ccggtatcaa tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc
     5401 ccctgcgagc ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc
     5461 gcatcggaac ggctgaccgc atacgggtta aaggaagatt cggtctgcat aattgccaga
     5521 atcagcgact catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg
     5581 tgcgcacgtt tatcaaggtg gttcggtacc atgttaatgg tgacgctgta gatgatacgc
     5641 agcccgttac tgcggctttg cagacggttt ttcagcagat aatccgcgaa gttgcttgcg
     5701 cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt
     5761 tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca
     5821 cccatcaata acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgccccggcg
     5881 atggtttcga tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga
     5941 tcggtgtatt tcacgtagtc cttaggacca gcgatcacca cctctttgaa gccccagatg
     6001 ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt gtctttgacc
     6061 caggcttcgt tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca
     6121 atcaaagcca gcgcgagata ttttttcatc attccaggtg cgtgttgtgt ttgtttttat
     6181 gcaagttgcc ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc
     6241 ctcgatatgc acctctttac cctcgaacag gaagttgacc atctcctgct caagcagctt
     6301 gcggtgctcg gcattcatca tgttgagttt cttttcatta atcagcatgg tttgcttgtg
     6361 ctgccactgc gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc
     6421 ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt
     6481 tctgctcata aatcatcctc tttatcgact cacgcgctaa accggcgtgc cagtgcgtaa
     6541 ctgctgtaac aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt
     6601 ataccagagc gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat
     6661 aggcacaata tctaagtgga aatggctgaa ggtatggcga aacgcggtca gctgcgtcag
     6721 gttatcggca gcaatctgcc gttgcgccag ccaatgccgc aaactttctt cgtcggcaaa
     6781 ctgcgggaaa cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac
     6841 ttcatcttcg tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg
     6901 ttttttgccc ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag
     6961 cggacagagc gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat
     7021 cgcctgatta aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa
     7081 tttattctcg acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac
     7141 ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc
     7201 gcctgcggtg gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa
     7261 tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc
     7321 gtaatagcca agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc
     7381 ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg
     7441 agtttgttgc aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca
     7501 gggcagcgtt ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga
     7561 cgcttgcatg gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca
     7621 gggtgtaaac cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac
     7681 tttggataat gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga
     7741 cgtcatttca ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt
     7801 gcgccgccag gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt
     7861 gatgggcgtt gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc
     7921 gccggtgacg cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga
     7981 tcgccctgag caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct
     8041 ggcttctgcg catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga
     8101 agtgctgcat aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga
     8161 cccgtggcac aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact
     8221 ggtaaaaagc aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta
     8281 tgcggaacat atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag
     8341 caatgattac gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca
     8401 tcgtcttggt cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac
     8461 cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg
     8521 gtggcatggc gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat
     8581 tttattaacg aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc
     8641 tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt
     8701 gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac
     8761 gtttggtggg actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta
     8821 cgcttatcag gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca
     8881 tccggcatgg aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt
     8941 ttttcttgag ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg
     9001 gcaatgaccg ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc
     9061 agcccgcaaa ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc
     9121 aacgtgatgt ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag
     9181 gattatcagg ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc
     9241 gtcgaaaaag cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc
     9301 aaaatgcgca gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt
     9361 atcgaaacgc gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc
     9421 gaaggccagc aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa
     9481 atgggcgcga aagcggtgtt gaaaagcggc ggtaacccat tacagaatgt gctgggaagc
     9541 ctgggggggc tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag
     9601 cagtttggca aagatgtttg tagccgtgtt gtgactctgg aagatagccg caaagccctg
     9661 gtcgggaatt taaaataatc ctctatttta agacggcata atactttttt atgccgttta
     9721 attcttcgtt ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta
     9781 aattgtttaa cgtcaaattt cccatacaaa gctaagggat aatgcgtagc gttcacgtaa
     9841 ctggaggaat gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt
     9901 tagtggtgca gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc
     9961 cggtggtggt gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa
    10021 tctggttaat gcggtgccgc aactgaagga cattgcgaac gttaaaggcg agcaggtagt
    10081 gaatatcggc tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa
    10141 caccgactgc gataagaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga
    10201 aactgcttac ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc
    10261 aatgcgtccg tccacgtcta tgagcgcaga cggtccattc aacctgtata acgcggtagt
    10321 gaccgcagct gataaagcct ccgccaaccg tggcgtgctg gtagtgatga atgacaccgt
    10381 gcttgatggc cgtgacgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt
    10441 taactacggt cctctgggtt acattcacaa cggtaagatt gactaccagc gtaccccggc
    10501 acgtaagcat accagcgaca cgccattcga tgtctctaag ctgaatgaac tgccgaaagt
    10561 cggcattgtt tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc
    10621 gggctatgat ggcatcgtta gcgctggtgt gggtaacggc aacctgtata aatctgtgtt
    10681 cgacacgctg gcgaccgccg cgaaaaacgg tactgcagtc gtgcgttctt cccgcgtacc
    10741 gacgggcgct accactcagg atgccgaagt ggatgatgcg aaatacggct tcgtcgcctc
    10801 tggcacgctg aacccgcaaa aagcgcgcgt tctgctgcaa ctggctctga cgcaaaccaa
    10861 agatccgcag cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt
    10921 gccggggctt tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa
    10981 tcaccggaat aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc
    11041 acggcactgc ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa
    11101 aatatcttaa aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc
    11161 accttaaaaa atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc
    11221 tcttccagtg acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa
    11281 cagacctgga cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg
    11341 cccgcgtctg gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg
    11401 cgggtggtcg acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc
    11461 aattatcgct ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg
    11521 ctggaaaagg aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat
    11581 gcatattggg gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa
    11641 attcaccaac agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa
    11701 tatgacaaag aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt
    11761 tatgaagtgc cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact
    11821 tttctgcaat cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa
    11881 gggcaatatc cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg
    11941 atgaaaattg acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg
    12001 cggcgcaatg gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag
    12061 ttatccggca aattattttt tacattatcc ctagtagctg tggaatgaac aaggatattt
    12121 gcggaatgta agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg
    12181 gtttgattaa atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac
    12241 ccaccggcgg tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta
    12301 ctgggtccat tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag
    12361 ccggtgtcat gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata
    12421 tgatcagtgg gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag
    12481 cattagtcat tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag
    12541 tggtaacaac cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa
    12601 ccagcaccga gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa
    12661 aaatgccgcc cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat
    12721 ccagaaacgc tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc
    12781 gtttagcaat aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat
    12841 atcccgccat aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta
    12901 acacgtttga gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag
    12961 aaaatgccgg atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag
    13021 cagctgccgc aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca
    13081 catgaccaag tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta
    13141 tcgcaatacc gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata
    13201 gggcaaagtt atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc
    13261 agggaaacat caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca
    13321 caccaataaa gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca
    13381 ttaactggcc tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa
    13441 ggcataaaac atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg
    13501 tatttgcatc gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc
    13561 accagtcaca atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag
    13621 aagtaattgc ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg
    13681 cgcgccgacg gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag
    13741 ctcatcggtt aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac
    13801 gcatacaacc agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc
    13861 aagtttcagg cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc
    13921 ccggaatatt attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct
    13981 tttttgaagt cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt
    14041 acaatttcac cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac
    14101 aatttttgtt gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct
    14161 ggggttaatt tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt
    14221 tcatcttcag aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg
    14281 ttattttctg cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa
    14341 attggagtgg gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga
    14401 acacgaattt tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa
    14461 ctgcgtgtac ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc
    14521 ataatttctt taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat
    14581 aagaacggca aattatagat agcataaata ttatcaaaag actccagatc actggcggaa
    14641 cctttggtca tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc
    14701 atctggctgc tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct
    14761 ttcgccagct cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac
    14821 tttaacgtca ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg
    14881 cagactaatg agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg
    14941 aaattggttg atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt
    15001 ctgacaactt gtctatgttt atcacaactt gaaataaagt cagaaattaa atattaaatt
    15061 tgtgcgccag gttgtacatg tttataatca ataaatg
//
LOCUS       DAZFFI010000102        15054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFFI010000102 DAZFFI010000000
VERSION     DAZFFI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.102"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..443)
                     /locus_tag="ACHJ12_003804"
     CDS             complement(<1..443)
                     /locus_tag="ACHJ12_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
                     /protein_id="HFO4991527.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSE"
     gene            complement(440..562)
                     /locus_tag="ACHJ12_003805"
     CDS             complement(440..562)
                     /locus_tag="ACHJ12_003805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991528.1"
                     /translation="MASKNTGLTASSKTHPEFMCGDIYRNEYGSHMTMISRGKK"
     gene            complement(620..823)
                     /locus_tag="ACHJ12_003806"
     CDS             complement(620..823)
                     /locus_tag="ACHJ12_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991529.1"
                     /translation="MQEITLAEASLQAQQVEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            complement(868..1077)
                     /locus_tag="ACHJ12_003807"
     CDS             complement(868..1077)
                     /locus_tag="ACHJ12_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991530.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            complement(1081..1266)
                     /locus_tag="ACHJ12_003808"
     CDS             complement(1081..1266)
                     /locus_tag="ACHJ12_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991531.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(1259..1723)
                     /locus_tag="ACHJ12_003809"
     CDS             complement(1259..1723)
                     /locus_tag="ACHJ12_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4991532.1"
                     /translation="MDKNMLIASSFGLYDAGVAHMATGFSSLNQIADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSYLASLLTPLRLATMFSSVVARLLNQQIGAFHMAVS
                     ARPYFVWRFMQCLTGSIAIFTVTAATEREARARLPHAHLIFVARIRQEVTHA"
     gene            complement(1716..1904)
                     /locus_tag="ACHJ12_003810"
     CDS             complement(1716..1904)
                     /locus_tag="ACHJ12_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4991533.1"
                     /translation="MATTPTLVHSQTAFIWRFIIFGASESQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQEASHG"
     gene            complement(1935..2786)
                     /locus_tag="ACHJ12_003811"
                     /pseudo
     CDS             complement(1935..2786)
                     /locus_tag="ACHJ12_003811"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            2842..3021
                     /locus_tag="ACHJ12_003812"
     CDS             2842..3021
                     /locus_tag="ACHJ12_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991534.1"
                     /translation="MSIHKSERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            3079..3327
                     /locus_tag="ACHJ12_003813"
     CDS             3079..3327
                     /locus_tag="ACHJ12_003813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991535.1"
                     /translation="MDDQKKNSFAMMVTLTSARITEHERKRKAAREQARATELTRKEL
                     NALAREHREAPEAFALDNFLKSTCRKKIRTSGPRGHSS"
     gene            complement(4221..4940)
                     /locus_tag="ACHJ12_003814"
     CDS             complement(4221..4940)
                     /locus_tag="ACHJ12_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991536.1"
                     /translation="MKRPNMPVQGQGFVQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSDRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFDEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVKHA"
     gene            complement(5080..5286)
                     /locus_tag="ACHJ12_003815"
     CDS             complement(5080..5286)
                     /locus_tag="ACHJ12_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4991537.1"
                     /translation="METIRKIIVRTEVKKLINIKSDSTLQAMIKAGEFPAGFRIGLRR
                     RGWYEDEVLAWLKEREQEARGAVA"
     gene            complement(5564..5791)
                     /locus_tag="ACHJ12_003816"
     CDS             complement(5564..5791)
                     /locus_tag="ACHJ12_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991538.1"
                     /translation="MQDTQHNSEGMQLALLVNREFWSTYDPEDKSTAPSSREVIDFLV
                     SRGASKNLAVSIDKVVRPASMKAGGRPRKWR"
     gene            complement(5926..7173)
                     /locus_tag="ACHJ12_003817"
     CDS             complement(5926..7173)
                     /locus_tag="ACHJ12_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4991539.1"
                     /translation="MARKTTPLTTTQIKAARPGEKELTLHDGGGLYLLIKPSGAKIWR
                     FSYYRPADKKRALMSLGSLDEIPLAEARRLRNEYRSLIASGTDPQDHERRKREAEARR
                     QGNTFENVAAAWYQMKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTAHRFVEIL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDSNPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMLAVAGASIERQTRLLIEWQLLTVTRPAEASSARWEEIDLKASTWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHREYVFPGLKDPRQPMNSQTANVALRRMGFGGI
                     LVSHGMRAIFSTAANEEGFEPDVIEAALAHVDMNSVRRAYNRSNYLEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAVQN"
     gene            complement(7380..7456)
                     /locus_tag="ACHJ12_003818"
     tRNA            complement(7380..7456)
                     /locus_tag="ACHJ12_003818"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7420..7422),aa:Pro,seq:cgg)
     gene            complement(7548..9239)
                     /gene="eptB"
                     /locus_tag="ACHJ12_003819"
     CDS             complement(7548..9239)
                     /gene="eptB"
                     /locus_tag="ACHJ12_003819"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4991540.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(9492..10016)
                     /gene="lpfE"
                     /locus_tag="ACHJ12_003820"
     CDS             complement(9492..10016)
                     /gene="lpfE"
                     /locus_tag="ACHJ12_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4991541.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            complement(10022..11077)
                     /gene="lpfD"
                     /locus_tag="ACHJ12_003821"
     CDS             complement(10022..11077)
                     /gene="lpfD"
                     /locus_tag="ACHJ12_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO4991542.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            complement(11088..13619)
                     /gene="lpfC"
                     /locus_tag="ACHJ12_003822"
     CDS             complement(11088..13619)
                     /gene="lpfC"
                     /locus_tag="ACHJ12_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO4991543.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            complement(13643..14329)
                     /gene="lpfB"
                     /locus_tag="ACHJ12_003823"
     CDS             complement(13643..14329)
                     /gene="lpfB"
                     /locus_tag="ACHJ12_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO4991544.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            complement(14413..14937)
                     /locus_tag="ACHJ12_003824"
     CDS             complement(14413..14937)
                     /locus_tag="ACHJ12_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO4991545.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
BASE COUNT         3743 a         3793 c         3488 g         4030 t
ORIGIN      
        1 tcctctgatt gttcggatgt ttttttcttt acaggcagct caataaccag tccgtcagaa
       61 atgtttttgc gctcagtctc cagatagtta cgccagttct gccgctcgtt atggtgcata
      121 ccttcggggt aatagtctgc gtcctgtacg cctgccgccg ccagcttctg acctatgcct
      181 ttgaccatca cctgatcaag gtgtccggcc ttgtatatcc gcgctgattt gcgcccgtcg
      241 ggcacaattt gcagattgcc aatatcttta agttgctcat cagccagcac gacaggcggt
      301 gttttatctc cggcaagttt tccgtcctgc tcctgccact gtttcgcgtg cgcccacgca
      361 tcactgccag cgaaaataat cacttccgta tcggcgtgtt ttttccctcg aggctgtttt
      421 tttaagttcg gtgcgctttt catttcttac ctctgctgat catcgtcatg tggctaccgt
      481 attcattgcg gtaaatatcc ccgcacataa attcggggtg agttttgcta ctggcagtta
      541 agccagtatt ttttgatgcc attgaattaa tctcctgaag gagtcgtctt aatctttatt
      601 tcccgtttat ttttagccgt tattgttcgc cgcgtttttc ttcctccgtc attaaccaca
      661 ccgccacatt gctggaaagt tgtttaatca gtcccgcaat aacagaaagg tcatggtcgt
      721 caatttcttc gtggttacgt tcaatcatgc gctcaatcat gcggctgatt acttctacct
      781 gctgcgcctg tagcgaggct tctgccagtg ttatttcctg cattactgca cccccttaat
      841 ttcatgatat tcagaacaac aaaggattca gtcattgacg catattgcat catcacccga
      901 tgccgcaatc agccagctac cagccttacc tgtaagctgc ttaatcaggg cgagcaatga
      961 ctcaatctca ccctcagtca tttcacctgt gttacccatc atcagagaac aaataagttc
     1021 tgcctgcatt gcgctttttg ctgcttcatc aacagtcata tcggaaaaat cacacatgaa
     1081 ttactcctca tcctgaatac atgtcactgc gtggataatt acgccggata aaccggcttt
     1141 agctgatttg ttaattactg tgctgattgc ggcctggacg ttttcggcct tcacgttctg
     1201 cgtgatgcca acggtatagc cacgcttgtt aacagcacgg gaaaagacgc ggaaggtttt
     1261 aagcatgagt cacctcctga cggattcggg cgacaaaaat cagatgtgcg tgcggcagcc
     1321 gggcgcgtgc ttcgcgctca gttgcagcgg taacggtgaa tattgcaatg ctgcctgtca
     1381 ggcactgcat aaagcgccag acaaagtaag ggcgtgcgga tacagccatg tgaaaggctc
     1441 ctatctgttg attaaggagt ctcgctacta cgctgctaaa catggtggcg agacgtaacg
     1501 gggttagcag actggccaga taggaaccag cgagcgcaaa ggctcccccg ttacgccccg
     1561 ccataatgcg ggtatgggta ggcttacgga cacaaaaaaa ccgcatatcg gaatgtaagc
     1621 ggctgtccgc tatctgattc aggctgctaa acccggtcgc catgtgggcg actccggcat
     1681 catacagccc gaaagaagag gcgatcaaca tgtttttatc catgtgatgc ctcctgacgg
     1741 atacgggcgg cgaatacagc aacgcaacct gacgggcaac ggttacgcgc ttcgcgttcc
     1801 gtccaggcgg ttacgtggat gatttgggat tctgacgcgc caaagatgat aaagcgccag
     1861 ataaaggcag tctgagaatg tacgagggta ggggtagtag ccatgttggc agcctcctta
     1921 cagtgatgaa taacgtcacc gcaggagttc ctacgctcac aatgggcggt gacactggcg
     1981 ggggtaggaa taccggactg taaggactcc ggcctacctt tcggtagccc cgccagcatc
     2041 accattgttt gctggtggcg ctgtgcaaaa tgcaactcgt gaaatgactg gatggtgcac
     2101 gagtacgcac aatacacaac gccattgata tggcgtaaag cgctggcaga aaaaaagacg
     2161 cttctggcgt catttatcgc cttacagtta aacgggttcc tacgcccgat ccccgtttta
     2221 taagggataa cctcagtata tgccgctttt ttgcttaagt ggtcggatgt gttgcgagaa
     2281 aagtttgtgc gaaaaatacc tgcgttttct ttgaaagcac ataataacat cacgttattt
     2341 atgtttatgc agtgtgtcgc aaagcaacct gaatcatcac gcaaattaca accataaaaa
     2401 atacctgcgc tgtgatatga ggtactacag cgcaccgctt tacgggtaca gacaatgaca
     2461 aatgaagaac tacaggaaat cgcattatcc atacttgagc gacagcgcga aaaagcacaa
     2521 cggcaaagga ggttgttact taatccactg ccactcacca ggccaacacg atttgccctt
     2581 gaggcttttg tcgagaaata cccacggcgg cttaaatccg gcaaaaaccg accgcctggc
     2641 tgatagcctg ccagcggttc gtgtgctgtt tgtatgtgta cagagtgtgg agtatgctgt
     2701 ctgacagcca taatcgttac tcctgatcgc gatttggtta gacgccctgc acgtatttca
     2761 gcactgcggg gcgttgtttt ttgcattgag tgtatgtaat gtgtagagac acataaacac
     2821 actacatgag gtgtaattaa cgtgtcaata cacaaaagcg aaaggcgtgg taatcccccg
     2881 ttccagttcc gtttagatcc tgacttacgg gaaaagatgg aaaaagcaca acaacaggac
     2941 ggtgatgatt ctcttgcagc ctggataaag cgaataatcc gcaaagaatt acagcaacgc
     3001 ggtatagaac ccaaaggata aaagccacca gcaccgtagt gtgaggaacc acggcgtatt
     3061 gttatgcagg aagacacgat ggatgaccag aaaaaaaact cctttgccat gatggttaca
     3121 ctgaccagcg cccgtataac tgaacatgaa cgaaagcgga aagctgcacg cgaacaggca
     3181 agagcaacag aattaacacg taaagaactt aacgctctgg cacgggaaca cagagaagca
     3241 cctgaagctt ttgcacttga taattttctg aaaagtacat gccgtaagaa aatacgaaca
     3301 tcgggaccaa ggggccattc gtcgtaaact gcatattgtc cgccagcttc agatatggca
     3361 ttgtggataa tgcgcttgat ttcctttatc tccactggcg accctttcgt ggtcgccttt
     3421 gttttgcctg ccattcctgc atcatgcgga tattcgcgcc gtacagggcc accagcgcga
     3481 taatttgata aaccctcctc caagtattgc ttaacgtgtt tcatgcgctc agtggcgaaa
     3541 tggctcacga tatggcgcat gtaatcatga gtaagctgct ggcgggattt tgcgccttgc
     3601 cagaaatcag agccaccgac cgccatgcaa tgccgtgtca tgttctgacg gtggtagcgt
     3661 gctggtggcc tgtaacggtt accagccatg tttacaggca tgataatcat cttatttact
     3721 cctgattttc gttattaaat tctgctgata cccttatata ttggggttgt ggtagtactt
     3781 tgcgcggtta aattatgagc aacatttcat ttgtgaaatt atgttttgaa aataaattcc
     3841 cttttgattc aacaccataa gttttattaa tttgactcgc gttatttttg catgttactt
     3901 ataggggatt tgagggggca tcccttacct gcatacccta catacctcaa caccgtgcca
     3961 acgccaacgg cggcccctat accagaaagg ccactccctc ccctggccaa ggccctggct
     4021 aaggctttgg tagttcaaac atgatgcaca aaccgaatga caacacctga agccagcact
     4081 gggggttgaa tggtaaattg cgcacaaaaa cagttagcag gctaattatt ataatttgaa
     4141 gcatttgact acccctctca tcccctgatg gctgtaaccc cgtgtataaa ccgccttcat
     4201 gattgggcgg gataacataa ttacgcatgc ttcaccccca gagacggcgc tgacgtcatc
     4261 aggctggcga atgtctccat gcggcgggca acttcccgca ccgcttcggt cggtgtcatg
     4321 ccgttctgta tcgcctcgtc aaatgctgcc tgctcgattt tctgcatgac tatcagtaag
     4381 cctaactgtt gctctcccgc gctctgacgc tcgcctacag ccagcccagc gaagcgattt
     4441 tcagtgcggg caaggttgat gtaatggtga cgttctgcgc ccagtgcttt aagcccgtca
     4501 cggctttcgt gatagcctgg cagatattca cggcgcacag gctcggcagc gcgatgtttt
     4561 gcgaatgcct tcgtaacatc cagcttgaac tggttgatac gttctgcgtc acgcccacgg
     4621 ataacgcgta acagatagtc aagctgtagc tcattcagca aataactaat ttcaggacga
     4681 ccgcgcttgc ggtcagattt tcccgatttc aaataggtta attccttgac tgactggcga
     4741 ggcagtgagc caagctcgcg caatctggat tgatgagatt taatcgtcgc aatgatactt
     4801 tcgtgttgta ttcctgctcg ctcagaaata acgcgggaat caatacgtga ctccgctttg
     4861 tggcgaatca atatatcgtt ctggctgatt tcaggctgaa cgaagccctg cccctgtacg
     4921 ggcatattcg gacgtttcat tgaaaaactc ctgaatgtgt tcctgattta ctccagcaag
     4981 attgcagtgt ctttatcgtg gcggtagcat gattgcttcc attgagtttt ctcctgtcac
     5041 tcagggtatt cagttgctga atgccctttt tatccccctt tatgcaactg caccgcgcgc
     5101 ttcctgttcg cgttccttca gccaggccag cacttcatcc tcataccatc cgcgacgacg
     5161 cagaccaata cgaaaacccg ccggaaattc cccagcctta atcatggcct gtagcgtgct
     5221 gtctgatttg atattgatga gtttttttac ttctgtgcga acaatgattt ttctgattgt
     5281 ttccatcgtg tactccttat tatccggctt attccggtga gaaatacggt aaaacggcta
     5341 aaccagaaaa aacagtaccc accgttttta aacggtaccc acccttttat cgttctgggg
     5401 tgaacaggtg aacactttgg tgaatagttt tacaaaactg ttcacccttt aacatattga
     5461 tactaaatac atttattaca gggtgaacag tggtgaatag tttttctata acttaatttt
     5521 tatcaccata aaaaaagcgg catttctgcc gcctcaggga gtgttaccgc cattttcttg
     5581 gcctgccacc agctttcatg gatgccggac gtacgacctt atcaatactt accgccagat
     5641 ttttggaagc accacgggat accagaaaat ctattacctc cctgctggaa ggggccgtgc
     5701 ttttatcttc ggggtcgtag gttgaccaga actcacggtt aaccagtagc gccagttgca
     5761 taccttcaga gttgtgttgt gtgtcttgca tattaacgga ctcatacctc tcggccttgt
     5821 cgttaaattt gctgtaatta taccacgtac tactacggtt tccgccgttt ttactgtttt
     5881 ttcgtacagt tctttgacag tcctgatatt tcctgggcgg tccgttcagt tctgtaccgc
     5941 ccgaatgccc cttttaccgc tggcaagcgt cacacctgtt gccgctgcct caacaaactc
     6001 accccaccag cgcatcagca caatgcgttt ttcaaggtag ttactcctgt tgtaagcccg
     6061 tctcacgctg ttcatatcca catgagcaag ggccgcttct attacgtccg gctcaaatcc
     6121 ttcctcgttc gctgccgtgc tgaatatggc gcgcataccg tgggaaacca gaatcccgcc
     6181 gaatcccata cggcgtaatg caacattggc ggtctgactg ttcattggct ggcgcgggtc
     6241 tttcaggcct ggaaaaacat actcgcggtg atggctgatt ggcttcatgg cttcaagtac
     6301 tgccatagcc tgatcacaaa gcgggataac gtggtcgcgg cgcatcttca tacgtccggc
     6361 ggggatcgtc catgtactgg ccttaaggtc gatttcctcc caccgtgccg atgatgcttc
     6421 ggcggggcgt gttactgtca acaactgcca ttcaatcagt agtcttgtct gccgttctat
     6481 ggatgcacca gcaacggcaa gcataagctc cggtaactgc tcaggcctga ttgtcggcat
     6541 atgttttttt acaggggagg gaaacgcttt acgcacattc atagccggat tgctgtcaat
     6601 cagcccgctg ttggcggcgt aatccattac ctcattaaca cgctgcaaaa cccgcttaag
     6661 tgtttccaga ttgccgcgct ctttgatggg ggtaagtatt tctacgaacc tgtgagcggt
     6721 gagggtgtct atcggggtat tgccgatgaa cgggaataca tatttatcca gtgaccgcca
     6781 gatgtcctta atcgtgttgg gggccagatt ctggctgatt ttcatctggt accaggctgc
     6841 cgccacattt tcaaacgtgt tgccctgtct gcgggcctca gcctcacgtt ttcgccgttc
     6901 atggtcctgc gggtcagttc ctgacgcaat caacgacctg tattcgtttc gtaaccttct
     6961 tgcctctgcc agggggattt catccagtga tcccaggctc attaacgcac gctttttgtc
     7021 tgctggcctg tagtaactga atcgccatat cttcgcccct gacggtttaa ttaacaggta
     7081 cagtccgccg ccatcatgca gcgtcagttc cttttcacct ggtcgtgcgg ctttgatctg
     7141 cgtggttgtg aggggggtgg tttttctcgc catgtactca cctgttgtgt gtgattatcc
     7201 ggttttagct atacggattt agctatatgg cagaccgtat agctaaacat atatctaaaa
     7261 aatccggtta taaccatccc tttcaggtga ttactggtga ctaaacaata caaaaaagcc
     7321 caccaatcat taaattagcg ggctttccgt gtcttaccgg tgattaccgg tggttaattt
     7381 ggtcggtgat agaggattcg aacctccgac cccttcgtcc cgaacgaagt gcgctaccag
     7441 gctgcgccaa tcaccgaatg cggggcgcat cttactgcgc agatacgccc tcgtcaatcc
     7501 cttaatagca aaatgccttt tgatcggcga gaaagtcagc aggccgctta gttagccgct
     7561 gccacttttg tctgcgggat gtgacaccag ttgttgtttt cgttaattcc accatccggt
     7621 gaagtatagc caagacaacc catgatggtg tcgtacagct ctacatgacg gcgtggcact
     7681 ttcatgtcgg cttctttttt cagctgcgca aatgcctgag cattggccgg attttccaga
     7741 tatttatctg acatccagac catcatcggt acgcggaact gctccggcgg tgccagttca
     7801 cgcggcgtgc cgtgcaggtg ctcgcgttca ttaattgact caccgtggtc agctgcgtag
     7861 aacacaatcg ctttcttatc gcgaacctga tcaatcacgc tggagataaa gtgatccaca
     7921 taggtcaccg agttgtcata ggagttgatc atctgcgctt tggtacagcc gctatcaaca
     7981 ccaatacatt ccggcttcca ctgcgcgaag ctacgcggat aacgctgggt gtagttaaaa
     8041 tgcgaacctt tggtatgcag aatgatcaga tgcttaccat ccgggttgcg ccctagcgat
     8101 tgctgcattt cgtctaccag caacatatca tctaccggct tgccacgatt acgtggctcc
     8161 gcaccaatct gctcacgata agcaatgttg tccgccatcg tgttgctgta gaaccacatt
     8221 tcgctctgca tagcgtagag gtcagaactg aatcctaact gcttcagcac cgcgaaaatg
     8281 ttctgttctt ttaatgtgcg ctgcggatta tcttccgcgc ccccctgacg tacaaacatg
     8341 caacgcagtg agagtttggt tgcggtatca caggagtaac cacggaatgc cgccagattt
     8401 ttctcctggg ccagcttcgg cgtggtatta cgctcatagc cgaaaatgcc catatggtcc
     8461 caacgcgtgg tttcaccgat gataaacacg acataagtgt catcaacgtt ttgcggtgcc
     8521 tggtacgtaa acttcttcgc cgggttaagg agagaattgt tatcggaaga ttcatcgacc
     8581 cgcgcccagg cgtacagccc caacgcagaa agccagtttg atggcagata agagttcgct
     8641 acgacaccgc cataactcgg caaatcaacg ccggtcgccc tctccacttt cttctgctgg
     8701 atatccagca aacgaatcgg tgcccaaacc ataatacccg ccagtacgac gaccgccagg
     8761 ctgcgaatac gctgccctgg ggttcgcagt tgtcggagca aggtgtagcg acagcggtta
     8821 ttccagataa ggataagagg caatgcacta acggcgatta accagagaat aaagttcaga
     8881 ccaacaactt cttttgacag gtcgatatcg gtggtcatga cggaagcgat gatgccataa
     8941 ccaatgacca cattaaggaa ggtcatgtaa tagctggcac ctgcggaaaa gagcaccacc
     9001 agcgacgcca gaatacgcca gctgcggcgg ccaaacagcg atagaagacg tagtaaaaag
     9061 aaggtcacca gtacggtggc ggccagttca acaacagcag aaacgccttt ccagacggta
     9121 aaatcgtgcg catagctgcc gaagcggcga taaaaaaccg cgccattcat aaaaaggcca
     9181 atatagattg caagcaaaaa gctcagcttc tgctgtgtaa tcgatttgat gtatctcatg
     9241 caaacaaacc ctggacaaac aggtgataac accctgctta gcggcttttc aggcccggca
     9301 ggggaaaagt gcggaggtag tcagataagt aaaatctaca cgcgcgaata agtagaccac
     9361 agcaaagcga aaaagtgtgg ggaaagagtg tgcatgaagc acaaatttac aaaaatgcaa
     9421 gtttgaaaag agactttagc gttacaaaaa gcagtaaaaa gggccagtta actggccctt
     9481 aagatgtgaa attattcgta ctgtacagta aagtcgaccg ttgcatctgc gcgaccaata
     9541 gttgcatcgg cgcgaatagg cacgtagctg gcgatatagt tcagttgatg tgcgccatca
     9601 ccgttaattg caaactcaac cggtgtcggc gttgtatttt ccgggaactg ttgtactcca
     9661 gcagcggttt tgattttcag cccaacgcct gtagcgccac ccgcatccag cgcaaaaaga
     9721 tttgagtcgt ctgcattggg tgtaccgtcc atagtcacgt aggctctgac cacttcgcta
     9781 cagtttttca ggccgatgga aaaaggagtg ggattaattt ccgttccgat tcttaccggc
     9841 tttttgagaa ccgcggtgcc catgtcaacc tcaacgctgt ctttcaccag ttcgcaagca
     9901 ccctggctga ttttaagggt gaatttcaat gttcctttcg gccctaaatc cgtggttgcc
     9961 gcgaatgacg aaaaggaaag aggcagcagg cataacgcaa gtattgttcg cttcatgact
    10021 gttactccag ttcgacatgt atggtagcaa taccgctata atccccggca gcgggtaatt
    10081 ttccggtagt gctaacggga taagcggtaa ttgtggttgt cgctttgcgt ttgacatctt
    10141 catacagcga gcctgggttc atttctaata agctattact gtcattcggc ttaagaatat
    10201 tacctttacc gtcctcaatg actgcaccaa catcggcgtt gcctaaatca attgcgctgg
    10261 gataggcagt atttggtgtt gcatcaatac tgagataaat tttgacgcca tctgaaatat
    10321 tattgcagtc aaagcttaat tctttttgta ccttctgaac attttgtggt ggttgtccgg
    10381 cacgcccttt aaaatcatgg gcctgatatt ctccgaatgg aatatccagt acggtgccag
    10441 cggcaagttc acagccttga gtaactgtaa tactcccgga aatactgaca ttggccatgg
    10501 gctgactatc attgtaaaca cctttctttt ttgtcccaaa gagggacgca actcgtgttt
    10561 taggaataat catttgccca acaaaaggat gtgaaatata aatactgata cttcctttac
    10621 cacctgtccc ccaattgtta gcagtatagg gcgtacattc tttctgtgcc gtgccattgc
    10681 ttcgatcggt aaaaggcacc gggatatcta attgtgtgta gacatatatt ttggtgagaa
    10741 tatcaatgta attattgaat ttatagtacc catctacagt tccaggctca ggcagaagcg
    10801 ttacaccttt aaaataagtc acaactccat tatctggaat gggatccggg cattcacaat
    10861 aagcggaata ttttgtacct aaagcaaagg gctgagttaa tattttccca gccttatttt
    10921 ctgaaggtac ctggatagtc tgtacgaaat caaaactata ctcatgggcc accccatttg
    10981 atgtctgaca tggtccccaa tccgcagccc atatatttcc agcaaaagtg ctcgtcccta
    11041 acagcaccat acctgccaac aaatttttaa ttttcatcta aatatcctta tttctcgcat
    11101 accgcattaa ccatcacgat agccgcattg tccttgttct ctggcaagcg gaatggcaca
    11161 cggcaacgtt gcgcctgctc gttcccccag ctcacctgca attcgccctc ttctggcatc
    11221 ccgctgatat aaagctgtcc ttcttcaccc acaatgctgc tggcaggttt gctctcatca
    11281 attaacgctg cggttgcacc aaacggtact gcgttgccat tcgcctgttt taagctcatc
    11341 agcacacggt aaccgacgcg ggtatcaaag cgggaacgca caactgcacc acgagtaggc
    11401 acgactttct ggaacgcttc ctgtagttca acgttttgcc ccaacgtgtc gctacgcaat
    11461 tccacaatgg tttcacgata tggtgtgaga taaggcacca cggcgttgcc catccagtca
    11521 acttctacgc cgctgctgtt aaccacatgt ccaccacgcg cgcccggtgc agaaacaatc
    11581 gccagacttt cgcctaaagg ctgcgaaagc gttatacctt cactgtgaac gatgacaccg
    11641 ccgcgcacgc catagttaac ctgttggtta tctttagaat agttatagcc tacgtcaatg
    11701 ttaccagagc cacccatata ccccatgttg acgctgccac tgtagcctac gccattactg
    11761 gtgtaaccct gctgaacgtt atagctcagg ttacgatctt ccagtaacgt gccgctaaca
    11821 gaggcctgtt gcgacgtctt gccattctgg tcggtagtca tgcgataact gctccacgcg
    11881 ccccccagag ggatggaaac actgaatgac cacaagcgat cgtcttcttc ccattccgga
    11941 cttctcgtcc aggtataggc cacgctgtaa ttcacccgac caatgtgacc gtgataaccg
    12001 gcgcttaagg agttttcctt accgtcaacg ttccagtaat cctgctgcgt catattgaaa
    12061 taaaccgagc cgtagtctcc cagttgctgg gtaatattgc cctgtatctg gctgcgcttg
    12121 tgataacggc ggtaatcact gtcggcgtca ctgcgaacat cagttgcttc ctggaaggtg
    12181 tagtagccgc tggttgagta tttgtagccc aacagacgca ggtcagtgcc accctcaaaa
    12241 cttttcgaat agaggaagcg ataagactgg ccttcctctt tttcattgtt gcgtaactga
    12301 cttttcgcct gagtgacatc caccgatact gcgccaatgg taccgaagtt tttccccagg
    12361 cccagagcta aagcgctgta atctgccgac aacaatgcac caccgtaggc ggtcataccc
    12421 cacggcaagc cgtacatggc ggtaaactgc ccgaaatgtg gtttatcgct gtcgtaatta
    12481 ccagcacgat attcaccggc agccagactg tatttcagat acccttcacg ctggaaaatc
    12541 gccaccgccg aaaacggctg gataaaacgt tgttcacttc cgtcggcttc tttaatgatc
    12601 accgtcaggt cgccgctgcc ggaggttgcg tagagatcgt taatctcaaa tgcacccggc
    12661 tgaacaaaag tgcgataaat gacatagccg ttctgttcga tactcacttc agcgttgctt
    12721 ttggcgatgc cgcgcactac cggagcaaag ccacgctggc tgtccggcaa catttcatcg
    12781 tcggatgcca gtaacgcccc acgcatttgt acgctatcaa aaatctcgcc aggcgtggcg
    12841 gtatcaccca gagttatctg tgatttaagt ggaataattg cccggtttaa cgatgtacca
    12901 acgttatccc attggttttt gccatcggta cggttccaga tactgttatt acgtaaacgc
    12961 cacggaccaa cgttcaaacc gctgcgcagg ttcaggtaat aactgtcact ggtcgaatct
    13021 gaagatgaac ctgtaccttc attgcgtcca ttgctgccac taaagctgta atccagcaac
    13081 agcgcaggta ttccttcgtc ccagcgagaa ggatcaaccg tgccgcgagc ggtcatcatc
    13141 attgccgcct gtggaaagga aagatgcagc gtctgagtat taaaatcaaa gcggctggtg
    13201 gcctggggaa tgatttcatc aaacgctacg caggcttctg gtggtgacat cttcagcgcc
    13261 ggaaagctgt caacgcgcac gcccatttct tccagttgca cccggctaag gcaaggcata
    13321 agcgactcac cgctggcttt gcgtttcgct tcgctggcgg tactaaaatc cagcgtccgg
    13381 gaagccattt tttcttcgtt tacaaacacc gaaacccggt atttaccggg gatttgctgg
    13441 ccgacaggtg agtcgtatac cgacaagtcg atgtgttgat cgataccctg aacgttttcg
    13501 agaaatttag ggttaaattt tccgccatac gcaggtaacg agctggcaat cgttaatgcc
    13561 agcagagaga aggaaaaatg cgctctgtag tgatagagag gtaaatgcat ccaatgcata
    13621 gtaaaagcgc cttgtaaaag gatcagttaa tcggcttcgt cagcatttcg ccagcaacac
    13681 cgaaatcatt aataatgctg tatttcactt cgtgcgcgtt gcctgtaccg gctggaaggt
    13741 taatgtttaa ctttcctttc gcgagaacca ttcccgcccg ttcaatcttt tgtccattaa
    13801 tggaaaactg attaaaggtc aggttataag cggagtcgtt attaaccaca atttggttat
    13861 tttgacgctg gaaacgtaac tgtttcatgg cctcgaggtt gcttcccggc aaaccttttg
    13921 gacgatagaa caacttcaaa cgggtgcgaa cggctatctg taagacgttt ttattttcat
    13981 tttcagcact tttcgcagga atggccttta cattaatcca gtaaacggat tcacgatctt
    14041 taggtaaggt atcttcaata ttgacgatac gtaagacgtt atttttatca ggatcaagtt
    14101 tgaataatgg cggtgtaata ataaacggtg tttttttatt gccattcaca tcatcaatcc
    14161 atgactggat caaaaactct tcatttttat tatggttttc aacggtcagc gacgcttctt
    14221 tcttgccgcc atcataaata acacgcgtcg tgcttaaggc tacgccacca gcaaaacact
    14281 gttgtgcggt cattaaaagt gccagcacag caaagctaat acgtttcatg attcggtccc
    14341 tgagtcattg gtgtgaataa tggcaacaca gtctctgttg ccattgagag atgatcgttc
    14401 cgtcaggatc gattagttgt aagtcaggtt gaagtcgact tcagcgttac cgtaacccgt
    14461 ggttactgca tctttggtag agacatagtt agcggtgaaa tacagaacgg tctgaccttc
    14521 tttcaggaca gtagcagatt taccagtatt caggtcaacc agggtattgg tgttatcata
    14581 aataccgata cctacgccag ttgcggcacc tggttcagtg cttacagaaa ccagtttgct
    14641 gttgtctgca tcgccaacgc cgttaaagct gatttccaca tttttgaaag tggagatatc
    14701 gcagttttcc agtttgattt tgaacggcgt tgctgtggat ttatcaccca tagcagtaaa
    14761 tacagacttg ttcacctgac ccagaacaac ttcttgtttt tgagtgtctg gagacacaac
    14821 acaagcggcg tcaacaatct caccggtgaa tttaacagta ccgtcgcccg catcagctgc
    14881 gaatacggaa gtagaggtca gcgcaagcgc agataaggca aacagaacct ttttcattta
    14941 taactccatt tatatagata aatatgcttg atgttgttca tacaacgggg cgcagattat
    15001 acaaatggtg ctaaaaaaga aaattatttt taatgaatat taattcttgc aaca
//
LOCUS       DAZFFI010000103        15036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFFI010000103 DAZFFI010000000
VERSION     DAZFFI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.103"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(74..2353)
                     /gene="maeB"
                     /locus_tag="ACHJ12_003825"
     CDS             complement(74..2353)
                     /gene="maeB"
                     /locus_tag="ACHJ12_003825"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4991546.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2642..3592
                     /gene="tal"
                     /locus_tag="ACHJ12_003826"
     CDS             2642..3592
                     /gene="tal"
                     /locus_tag="ACHJ12_003826"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4991547.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3612..5615
                     /gene="tkt"
                     /locus_tag="ACHJ12_003827"
     CDS             3612..5615
                     /gene="tkt"
                     /locus_tag="ACHJ12_003827"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4991548.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5711..6754)
                     /gene="ypfG"
                     /locus_tag="ACHJ12_003828"
     CDS             complement(5711..6754)
                     /gene="ypfG"
                     /locus_tag="ACHJ12_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4991549.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6880..7455)
                     /gene="nudK"
                     /locus_tag="ACHJ12_003829"
     CDS             complement(6880..7455)
                     /gene="nudK"
                     /locus_tag="ACHJ12_003829"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4991550.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7523..9502)
                     /gene="aegA"
                     /locus_tag="ACHJ12_003830"
     CDS             complement(7523..9502)
                     /gene="aegA"
                     /locus_tag="ACHJ12_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4991551.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9708..11408
                     /gene="narQ"
                     /locus_tag="ACHJ12_003831"
     CDS             9708..11408
                     /gene="narQ"
                     /locus_tag="ACHJ12_003831"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4991552.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11572..14685
                     /gene="acrD"
                     /locus_tag="ACHJ12_003832"
     CDS             11572..14685
                     /gene="acrD"
                     /locus_tag="ACHJ12_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4991553.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGGAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14784..14843)
                     /gene="ypfM"
                     /locus_tag="ACHJ12_003833"
     CDS             complement(14784..14843)
                     /gene="ypfM"
                     /locus_tag="ACHJ12_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4991554.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3519 a         3995 c         3925 g         3597 t
ORIGIN      
        1 aagctttact aatatattgc agccaaaccg cttaccctga atattcaggg taagcgtgag
       61 agttaaaaaa gaattacagc ggttgggttt gcgcttcaac cacggccagc gccaccatgt
      121 tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg aaccggtttc gccacgccca
      181 tcagcaccgg accgacagtc acaccttccg agctggaaac gcgcagtaag ttgtaactaa
      241 tgcgggcagc ttccatgttc ggcatcacca gaatattggc agaacctttc aaagggctgt
      301 ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc atcgccgtgc atttcaccat
      361 caatcatcag atctggcgca cgatccctga ccagttccag cgcctgacgc attttgctcg
      421 acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa cagagcaacg cgcggctcaa
      481 taccaaaacg acggacagtt tctgccgcca tcaaggtgat ctccgccagc tcttctgcgt
      541 ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt accactcggc agcagcagcg
      601 cgttcattgc acctgcggtg tgaacgccat cgcgataacc aaagacattt ttcaccacgc
      661 taaaatgttc atgataatcg cccaccgtac cgcagatcat tgcatcggct tccccacgct
      721 gaaccatgat cgcgccgatc actgtcgggt tactgatcag cgcccgctgc gcctgttcct
      781 gagtgacacc gcgacgcttc atgatctgga agtattcggt ccagtactct ttaaagcgcg
      841 gatcggattc gttattgacg atctcaaaat caacgcccgc tttgatctgc aagcccagtt
      901 tctgaatgcg catttcgatc acgttcggac gaccgataag gatcggtttc gccagtccca
      961 gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc ttccccttcc ggcagaacta
     1021 cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg ttggttttgt
     1081 aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga cgcgtcgcca
     1141 cgcccgactc catcgccgct ttcgccaccg caggagcgat cttaacgatc aagcgcggat
     1201 caaacggttt tggaatgatg tattccggac caaagctcag atcctgatcg ccatacgctg
     1261 aagccaccac ttcgctctgt tccgcatggg ccagttctgc aatcgcgcgt accgccgcca
     1321 gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg aagatgaacg
     1381 ggaagcaaag gacgttgttg acctggtttg gatagtcaga acgaccggtg cagatgatgg
     1441 catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg ttcgccagcg
     1501 ccaggatcat tggcgcacga gccattttct tcaccatttc ctgggtcagc actttcgggc
     1561 cggaacatcc gaggaaaata tccgcgcctt caatcacatc atcgagggta cgtttgccgt
     1621 catccaccac cgcatatgcg gctttggttt ccgccatgtt tggctcacgg ccctgataga
     1681 taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagacccagc gctaccagca
     1741 ggttcataca ggcgattgct gcggcacccg cgccggaaac caccatccgc acgtcggaga
     1801 tgtttttctc caccacgcgc aagccgttga ggatggcggc agtgctgatg attgccgtgc
     1861 cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc tgttcaatat
     1921 agaaacattc tggcgcttta atgtcttcga ggttgattcc gccgaaggtc ggttcgagcg
     1981 cagcgacaac ttcaataaat ttgtccgggt cgagttcatc aacttcaatg tcaaatacat
     2041 caatcccggc gaatttctta aacagaacgc ccttgccttc catcaccggt ttgccagcca
     2101 gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc gccaccaggt
     2161 taccgcgcgc ggtatatttg taggctttta acgggtcttt ttcgatttca agacaaggtg
     2221 cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg cgttgccaga ggcttggttg
     2281 gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca ctttgtttta
     2341 actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt gtgtgggcaa
     2401 cagcccctgc ccacaacgtg gcgcacatta ttaccctgcc ggagtctaca gactttgagc
     2461 aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac actgatgtta
     2521 cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg tttagcgaaa
     2581 tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag gagtatttcc
     2641 tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca gcggcgatat
     2701 tgagtccatt cgccattatc atccccagga tgccaccacc aatccttcgc tgttactcaa
     2761 agctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct ggggtaaaaa
     2821 aaatggcaag acccaggaac aacaggtggt cgcagcgtgt gacaaactgg cagtcaattt
     2881 cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca acagaagttg atgcacgcct
     2941 ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact tgtatcagca
     3001 acaaggcgtt gagaaatcac gcattttgat caaactggct tcgacctggg aaggaattcg
     3061 cgcggccgaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc tgttttcttt
     3121 tgcccaggcg cgggcctgtg cagaagcggg cgtttttctg atttcgccgt ttgtcgggcg
     3181 tatttatgac tggtatcagg cacgcaagcc gatggacccg tatgtggtgg aagaagatcc
     3241 gggcgttaaa tcggtgcgca atatctacga ctactataag caacaccact atgaaaccat
     3301 tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg gctgcgatcg
     3361 actgactatc gcaccgaatt tgctaaagga gctgcaggaa aaagtttcgc cagtggtacg
     3421 taaattaatc ccaccttctc agacgttccc acgcccagct cccatgagcg aagcggagtt
     3481 ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag gcattcgtct
     3541 gttcgccgtt gatcaacgca aactggaaga tcttcttgcc gccaaactat aaaccagcca
     3601 cggagtgtta tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc agtatggatg
     3661 cggtacaaaa agccaattct ggtcatcccg gcgcgccgat gggcatggct gatattgccg
     3721 aagtgctgtg gaacgatttt cttaaacata accctaccga cccaacctgg tatgatcgcg
     3781 accgctttat tctttccaac ggtcacgcgt cgatgctgct ctacagtttg ctacatctga
     3841 ccggttacga cctgccgctg gaagaactga agaacttccg tcagttgcat tcgaaaaccc
     3901 caggccaccc ggagattggt tatacgccag gcgttgaaac caccaccggc ccgcttggac
     3961 aaggtttggc gaacgccgtc gggctggcga tagcagagcg tacactggcg gcgcagttta
     4021 accagccaga ccatgagatc gtcgatcact tcacctatgt gtttatgggc gacggctgcc
     4081 tgatggaagg tatttcccac gaagtctgtt cgctggcagg cacgctggga ctgggcaagc
     4141 tgattggttt ttacgatcac aacggtattt ccatcgacgg tgaaactgaa ggctggttta
     4201 ccgacgatac ggcaaaacgt tttgaagcct atcactggca tgtgatccat gaaatcgacg
     4261 gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg aaagataagc
     4321 cgtcgctgat tatctgccgc acagtaattg gcttcggttc gcctaacaaa gcgggtaagg
     4381 aagaggcgca tggcgcaccg ctgggtgaag aagaagtggc attggcacgg caaaaactgg
     4441 gctggcacca tccgccattt gaaatcccta aagagattta tcacgcctgg gatgcccgcg
     4501 aaaaaggcga aaaagcgcag cagagctgga atgagaagtt tgccgcctat aaaaaggctc
     4561 atccgcaact ggcagaagag tttacccgtc ggatgagcgg tggtttaccg aaggactggg
     4621 agaaaacgac tcagaaatat atcaatgagt tgcaggcgaa tccggcgaaa atcgctaccc
     4681 gtaaggcttc gcaaaatacg cttaacgctt acgggccgat gctaccggag ctgctcggcg
     4741 gttcggcgga tctggctccc agcaacctga ccatctggaa aggttctgtt tcgctgaagg
     4801 aagatccagc gggcaactac attcactacg gggtgcgtga atttggcatg accgctatcg
     4861 ccaacggcat cgcgcaccac ggcggctttg tgccgtatac cgcgacgttc ctgatgtttg
     4921 ttgaatacgc ccgtaacgcc gcgcggatgg cggcactgat gaaagcgcgg cagattatgg
     4981 tttataccca cgactcaatt ggcctgggcg aagatggtcc gacgcaccag gctgttgagc
     5041 aactggccag cctgcgctta acgccaaatt tcagcacctg gcgaccgtgc gatcaggtgg
     5101 aagcggcggt gggctggaag ctggcggttg agcgccacaa cggaccgacg gcactgatcc
     5161 tctcaaggca gaatctggcc caggtggaac gtacgccgga tcaggttaaa gagattgctc
     5221 gtggcggtta tgtgctgaaa gacagcggcg gtaagccaga tattattctg attgccaccg
     5281 gttcagagat ggaaattacc ctgcaagcgg cagagaaatt agcaggagaa ggtcgcaatg
     5341 tacgcgtagt ttccctgccc tcgaccgata ttttcgacgc ccaggatgag gaatatcggg
     5401 agtcggtgtt gccttctaac gttgcggctc gcgtggcggt ggaagcaggt attgccgatt
     5461 actggtacaa gtatgttggt ctgaaagggg caattgtcgg gatgacgggt tacggggaat
     5521 ctgctccggc ggataagctg ttcccgttct ttggctttac cgccgagaat attgtggcaa
     5581 aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg atcgccggat gctgattgcc
     5641 ggatgcgacg ctgacgcgtc ttatccggcc tacagcgtct tatccggcct acatgtcccc
     5701 gcaattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
     5761 cgcaggtggg ttctgcggca taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
     5821 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg
     5881 atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga
     5941 atggcaaacg caaccgaacc gggcgcgagg ctagtggctt tttacgcgac acaatccatg
     6001 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagc gctttatcat
     6061 cagtcagcgc agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat
     6121 tcatccgcca gtttccataa tccagcaaat cgttgcgttc ttcgagtgag agtggcgtcg
     6181 gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagcggcg
     6241 gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa
     6301 taaacaacaa cgccgctttt aagccactca gagaaatggt ctgattgccg tcgcgtaagg
     6361 tgatcgcctt cccttcctga atcatctgca aaaacgcggt gatggttgcc gtatcatcgg
     6421 ttactaataa ccatggtgaa atccgccact tgtcaccact taacgccaac ggctcaccat
     6481 ctaacagcag ccgtggcgct atctctcctt cagacgcatc cggcgacttc aacccgccgc
     6541 gctcaatacg taaaacggca tcggtatgcg ccccggcgct gcggctcagg gtcatcacca
     6601 gtccattatg atcgcccgta ttgcgcgcca cgcagaagtt ttggttatta caggtgacct
     6661 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
     6721 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
     6781 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcag atttgtcgga
     6841 ttgagccaat aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacgt
     6901 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat
     6961 ctccagcgcc tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc
     7021 accggcgtta gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt
     7081 cacaccgcca ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc
     7141 cgtctcttca atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc
     7201 ggcgcaggtt tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa
     7261 ctgacgaatc agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt
     7321 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
     7381 gtaggtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
     7441 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatctta cgctgtgaag
     7501 tcgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
     7561 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
     7621 gcacggcgtc gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
     7681 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
     7741 gcatcccgtg tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt
     7801 cactgccctc aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg
     7861 tgcgcaggaa acgaattccg caaacgtgac cttgttcatt cagctcaagc gccaccggct
     7921 ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc
     7981 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
     8041 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
     8101 cggcggtatt gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt
     8161 tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
     8221 ctttcatgga acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
     8281 aatccaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
     8341 tgaagatttc ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
     8401 aagtgagcaa gccaccaatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc
     8461 gggtcagaac atccgcacag gccagccctg ccgggcctgc cccgataatc gccacccgct
     8521 tgtccacttt ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg
     8581 aaatatagcg ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg
     8641 caccttcaca caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg
     8701 tctggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
     8761 gcggtatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa
     8821 ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa
     8881 aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct
     8941 gcatttgttc gactttactc attaccggca tttcttgcgc cgcggtactg gcatgccacg
     9001 gttgatgttc ctgacgcgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga
     9061 cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc aacacaggca ggaccgtttt
     9121 cgcgccccgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg
     9181 gtgtcaggac gatttgcatc gtaccaaaag gacaggccac cacgcaggat ttacagccaa
     9241 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
     9301 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgtt
     9361 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
     9421 cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt
     9481 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
     9541 atatgagtgt tgttattacc cgacttacag gggaatcggc aatgttcatg tgcccagaat
     9601 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgt
     9661 gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
     9721 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
     9781 ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata
     9841 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc
     9901 cacaactcaa tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca
     9961 atctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca
    10021 actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata
    10081 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac
    10141 gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg
    10201 tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    10261 ccgccagtca gcgtattgaa cacggacagt tcgactcgcc gccgctggat accagtctgc
    10321 cgaatgagct tggtctgctt gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat
    10381 tgtacctttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    10441 gtcgtctgga ggtgttgtat cagtgttcgc aggcgctgaa caccagccag attgatgtgc
    10501 attgtttccg ccatattttg cagattgttc gcgataatga agcggctgaa tatctggagt
    10561 taaatgtcgg tgaaaactgg cggattagcg aagggcaacc aaacccggaa ttgccgatgc
    10621 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    10681 acgtttcatc atcagaaccg ctgcttaaca gcgtttcgtc gatgctggga cgcggtttgt
    10741 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcaacca
    10801 tcgcccgcga attgcacgac tcgctggctc aggtactttc ttacttacgt atccagttga
    10861 cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt
    10921 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    10981 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    11041 atcaaaccag cgccaaactg accctcgact gccgtctgcc aaccctggcg ctggatgcgc
    11101 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    11161 ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    11221 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggtc
    11281 tgaatatcat gcgcgaacgc gcggaacggc tgggtgggac gctgactttt tcacaacctt
    11341 ccggcggcgg cacgttagtg agtattagct ttcgctccgc ggagggtgag gaaagtcagc
    11401 tgatgtaatg cctcctactg accaaagaat acttgcactt aaggttcagt ataaaagggc
    11461 atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag
    11521 tgaagcaagt caagcctaca acgatacgca gaaacacgag gtcctctttt aatggcgaat
    11581 ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt
    11641 accctggcga ttttctcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg
    11701 cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag
    11761 gttatcgagc aaaatatgac cggcctcgat aatctcatgt atatgtcatc tcagagcagt
    11821 ggcaccggtc aggcatctgt tactttaagt tttaaagcag gcaccgatcc ggacgaagcc
    11881 gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa
    11941 aatcagggcg tgacggtgcg taaaaccggc gataccaata ttctgaccat tgctttcgtc
    12001 tctaccgatg gttcgatgga taaacaggat attgccgatt atgttgccag taatattcag
    12061 gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc
    12121 atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc
    12181 actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct
    12241 tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca
    12301 gaacagttcc gcgatatcac cttgcgggtg aatcaagacg gctcagaggt aaggctgggc
    12361 gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt
    12421 aagccagcct ccgggctggg ggtaaaactg gcctccggcg caaacgaaat ggcgacagcg
    12481 gagctggtgc tcaatcgtct cgacgagctg gcgcagtact tcccgcacgg actggaatac
    12541 aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg
    12601 ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc
    12661 gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt
    12721 tacgcctttg gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt
    12781 ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa
    12841 ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc
    12901 gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc
    12961 ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg
    13021 gtagcgatga tcctcactcc ggctctgtgt gccacactac ttaagccact gaaaaaaggt
    13081 gagcatcacg ggcaaaaagg cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc
    13141 gaacgctacg aaaaaggggg ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt
    13201 tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta
    13261 ccgctggaag accgtggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa
    13321 caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac
    13381 aacatcatgt cggtgtttgc cactgttggt tctggccccg ggggtaacgg gcaaaacgtg
    13441 gcgcgaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg
    13501 tttgccatta tcgagcgtgc aacaaaggcg tttaacaaga ttaaagaagc tcgcgttatc
    13561 gccagcagcc cgccagcaat tagcggtctt ggtagttctg cgggttttga tatggagttg
    13621 caggaccacg ctggagcagg tcacgatgcg ctgatggcag cacgtaacca gttgctggcg
    13681 ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga cgacagtccg
    13741 cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat
    13801 attaacgaca cactgcaaac cgcctggggt tcgagctatg ttaatgactt tatggatcgc
    13861 ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac
    13921 atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg
    13981 acctcacgct gggagacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt
    14041 gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa
    14101 tcgttagtga agcagttgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag
    14161 gagcggcttt ccggcgcgca ggctccggcg ctgtacgcta tttccttgct ggtggtattc
    14221 ctgtgtctgg cggcgttgta tgaaagctgg tcggtgccgt tctcggtaat gctggtcgtg
    14281 ccgctggggg ttatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt
    14341 tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc
    14401 gtcgagtttg ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac
    14461 gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta
    14521 ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc
    14581 gtaatgggcg ggatgatttc ggccactatt ctggctattt acttcgtgcc gctgttcttt
    14641 gtactggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg
    14701 acatgctaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg
    14761 ctgtttgtac tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag
    14821 ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa
    14881 caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa
    14941 accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt
    15001 cgagatattc atacggcatc taatactgat ttaatt
//
LOCUS       DAZFFI010000104        14932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFFI010000104 DAZFFI010000000
VERSION     DAZFFI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.104"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..374
                     /gene="yjbE"
                     /locus_tag="ACHJ12_003834"
     CDS             132..374
                     /gene="yjbE"
                     /locus_tag="ACHJ12_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4991555.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            488..1126
                     /locus_tag="ACHJ12_003835"
     CDS             488..1126
                     /locus_tag="ACHJ12_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4991556.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1123..1860
                     /locus_tag="ACHJ12_003836"
     CDS             1123..1860
                     /locus_tag="ACHJ12_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4991557.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1860..3956
                     /locus_tag="ACHJ12_003837"
     CDS             1860..3956
                     /locus_tag="ACHJ12_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4991558.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4003..4281)
                     /locus_tag="ACHJ12_003838"
     CDS             complement(4003..4281)
                     /locus_tag="ACHJ12_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991559.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4496..4906
                     /gene="psiE"
                     /locus_tag="ACHJ12_003839"
     CDS             4496..4906
                     /gene="psiE"
                     /locus_tag="ACHJ12_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4991560.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4950..6425)
                     /gene="xylE"
                     /locus_tag="ACHJ12_003840"
     CDS             complement(4950..6425)
                     /gene="xylE"
                     /locus_tag="ACHJ12_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO4991561.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6797..7687)
                     /gene="malG"
                     /locus_tag="ACHJ12_003841"
     CDS             complement(6797..7687)
                     /gene="malG"
                     /locus_tag="ACHJ12_003841"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4991562.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7702..9246)
                     /gene="malF"
                     /locus_tag="ACHJ12_003842"
     CDS             complement(7702..9246)
                     /gene="malF"
                     /locus_tag="ACHJ12_003842"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4991563.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9400..10590)
                     /gene="malE"
                     /locus_tag="ACHJ12_003843"
     CDS             complement(9400..10590)
                     /gene="malE"
                     /locus_tag="ACHJ12_003843"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4991564.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10955..12070
                     /gene="malK"
                     /locus_tag="ACHJ12_003844"
     CDS             10955..12070
                     /gene="malK"
                     /locus_tag="ACHJ12_003844"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4991565.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12142..13482
                     /gene="lamB"
                     /locus_tag="ACHJ12_003845"
     CDS             12142..13482
                     /gene="lamB"
                     /locus_tag="ACHJ12_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4991566.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13726..14646
                     /gene="malM"
                     /locus_tag="ACHJ12_003846"
     CDS             13726..14646
                     /gene="malM"
                     /locus_tag="ACHJ12_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4991567.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3703 a         3843 c         3915 g         3471 t
ORIGIN      
        1 tcattccaat tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta
       61 gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa
      121 gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga
      181 cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg
      241 tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg
      301 cgctcgcggc aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca
      361 ccagtaccca gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct
      421 tgaagtatga agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag
      481 aagagtcgtg aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc
      541 cacgactaaa gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca
      601 gctgacggac gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg
      661 cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac
      721 tcaggatcaa gccactcttg tcatacaaca tggtcgtctg gtgaagactt tgcttggcgg
      781 cgacaacctg attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat
      841 tgttgatggt gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta
      901 cgccaccgca cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga
      961 tgaaaccccg gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa
     1021 ccgctactgg atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga
     1081 ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt
     1141 gtcgcattga ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta
     1201 ttcagcaatg gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg
     1261 gtaggccagc cgcggctggc aaacagttgg tggcctggtg cggtgattag cgaagagctg
     1321 gcaacggcgg cagcattgcg gcagcagcag gcgttgctga cacggctggc agaactggct
     1381 gcagattcca gcgccgacga tgccgccgcg attaacgcct tacgccagca aattcaggcg
     1441 ttgaaagtga cgggcaggca aaaaatcaat cttgatcccg atatcgtccg cgttgccgaa
     1501 cgcggtaacc caccgttgca gggcaactac acgctgtggg tcgggccacc accgtccacg
     1561 gtcacgctgt tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgcgac
     1621 gtggcgagct atctctccgg gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg
     1681 gtggtttacc cggacggacg cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac
     1741 gtagagccga tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag
     1801 acgcctgatg cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa
     1861 tgaaaaaaag acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg
     1921 aaacatatcc tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac
     1981 aaactcccac cgcgcgtatg gcacgggaag gggagttgag tctgaactat cgcgataacg
     2041 atcagtaccg ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc
     2101 gctacaccga cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa
     2161 cgtataaaga taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc
     2221 cgcaagtggc ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc
     2281 ttgttgccag caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc
     2341 tgggcaccag cggtaatgta aaaaatccgc tctgttcagc cagtgataaa tattgctatc
     2401 gcgataacag ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg
     2461 cctcactgtt tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg
     2521 agtatgaagg caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt
     2581 ttaacgtcgg tgcgatttat cgcgttaccg attgggctga cgttaacctt agctatgaac
     2641 gtggcaacac ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt
     2701 cttacaacga taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc
     2761 attcggtggt ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc
     2821 agatccaggc gaaaggcgat acgctgtatg tcaccggcga gcaggtgaaa tatcgtgatt
     2881 cgcgcgaagg gatcatccgt gctaatcgga tcgtgatgaa cgatctgccg gatgggatca
     2941 aaacgatccg cattacggaa aatcgtctta acatgccgca ggtgacgacg gaaaccgatg
     3001 tcgccagcct gaaaaatcat ctcgccggag agccgttggg ccacgaaacg aagctggcgc
     3061 aaaaacgcgt cgagccagtg gttccgcagt ccaccgagca gggctggtat atcgacaaat
     3121 cacgctttga tttccacatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact
     3181 tttacatgta tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctgc
     3241 tgaccaccgg cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacca
     3301 atccgccgca ggactcgcac ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga
     3361 acgatgtcta tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct
     3421 acggtcaggt ctatggaggt tatctcgaaa ccatgtttgg cggtgcgggg gccgaagtgt
     3481 tgtatcgtcc gctggacagt aactgggcgt ttggtctgga tgccaactac gttaaacagc
     3541 gcgactggcg tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac
     3601 atctgaccgc ctactggacg ccatctttcg cccaggacgt gttggtgaaa gccagcgtcg
     3661 ggcagtatct ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg
     3721 gcgtggtggt tggcggctat gccacgatca ccaacgtttc gaaagaggag tacggcgaag
     3781 gggacttcac caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga
     3841 cacgcagccg tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg
     3901 ggcgtaagtt ccagttgtat gatatgacca gcgaccgtag cgtaaatttc cgctaagtca
     3961 tgggaaaggt gccagttttc gcactcactg gcaccctcat tcttaatgac ctgttgagcc
     4021 atcaggaacg cagttcaccc ccggtttacg atctggtgtg taaaggcgaa aacattctgt
     4081 cttcctgtcc gtcgatatcg gttgcaggtc tggttgcctg agcgactggg ccatcattgg
     4141 gctggagaca ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata
     4201 agcaggaaga acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac
     4261 ctttatcagg ttacgtttca tttgttccag aggaacattg tcgatttttc gcgcattgct
     4321 ggtggctggg aatcacctga atgggtgatt ttttggggct gtgtgttttg ccagatgcga
     4381 cgttgatggg agatgcccac ttaatcaagc tgaacaaaac atacacaaaa aatatagata
     4441 tccgtcacat ttttgcgtta tacaggaagc tcgccactgt gaaggaggta ctgctatgac
     4501 gtcactctct cgtccgcgcg tggagtttat ctccaccatt ttgcagaccg tactcaatct
     4561 tggcctgttg tgcctcggcc tgattttggt tgtcttcctc ggcaaagaaa cggtgcatct
     4621 ggcagatgtg ctgttcgcgc cagaacaaac cagcaaatat gagctggtag aaggactggt
     4681 ggtttacttt ctctatttcg aatttatcgc gctgattgtg aaatactttc agtccggttt
     4741 tcacttcccg ctacgttact ttgtctatat tgggatcacc gccattgtgc gcttgatcat
     4801 cgtcgatcat aaatcgccac tggatgtgtt gatctactcg gctgcgatcc tgttactggt
     4861 gatcactctg tggctgtgta attcgaaacg gctgaagcgg gagtaaaaag acagcacgcc
     4921 gaactggcgc ggcgtgctgg acaggaagat tacagcgtag cagtttgttg tgttttcttc
     4981 gtttccggtt cccagagggc ttccagctcc tcaagggttt tacctttggt ttccgggaca
     5041 aatttccaca taaatagtgc tgccagaacg cccatacacc cgtaaatcca gtaggagaaa
     5101 ccgttgtgga aatgggccac cagccaggag tttttgtcca tcatcgggaa ggtccaggag
     5161 acgaagtagt tcgccagcca ctgggccgcc accgcgattg ccagcgcttt accacgaata
     5221 gcattcggga agatttccga cagcagtacc cagcataccg gaccccagga catggcaaag
     5281 gcggcaacat agaacagcat cgacagtagc gccacaatac ccgatgcctg agtgtaaaac
     5341 gcggtaccga ggctaaacat accgattgcc attccgagtg cgccgataat ttgcagtggc
     5401 ttacgaccaa atttatccac cgtcataatt gccagtacgg taaaggtgag gttgataact
     5461 ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg cccccagcgt tttgaacact
     5521 tccggcgcgt agtacagcac cacattgatg ccgacaaatt gctggaagat ggagagcata
     5581 acgccgatta caatcacgcc cacgccaaac atcagcagac gaccaccggt tttgcggcca
     5641 tgatccaggg agtgtttaat ttcctgtact gcctgagttg caagcgtgtt gcccataatt
     5701 ttgcgcagga tactttccgc ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt
     5761 tctggcacgg tatacagcag cattaagaac agcagtgcag ggatacattc cgaggcaaac
     5821 atataacgcc agccgtcagt attcagccag ctggcatcac cggaacgggc aataaaatag
     5881 tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt
     5941 tttccgcgaa tatgagctgg agccagttcc gcaatataca ttggtgaaag cattgaggct
     6001 aaaccaacgc caataccgcc aataatacga taaataacaa attccgggac ataacctgcc
     6061 agataaatag ggactgtgtt gtccgggttt atagaggtaa aaccaagttc tggccaggca
     6121 gaacctacac cagaaataaa aaacaggaca gcagcaatct taagtgaatc acgacggccg
     6181 aagcggttac tgcaataacc accgagggca ccgccgatga tgcaaccaat cagagcgctg
     6241 gctacgcaaa atcctaacag ggagttggca gcggattcac ttaagttttg tggagcaaca
     6301 aagacggtat tgagtgactc aacagtaccg gaaataacgg cggtgtcgta gccaaataat
     6361 aaaccaccta atgtagcgac taaggtaatc gaaaatatat aactggaatt atactgggta
     6421 ttcattcaga cctgccttag accattctga tcttttatca gtgcattgat gttggaattg
     6481 tcaggcagtt aaggcaatta tcatttttta caatgcgttt acgtaatgtc ttttctgtga
     6541 tcttaattgt gataattatc aaaaattgat gaaaacaata ctattgccgt gactcagagc
     6601 acgaaagaga attatcgtaa gtgggaaaac aaataacgta aaaataataa gctctatgat
     6661 gggaatatta accggcgaac gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg
     6721 agcgccgaat aacagtcaca agttgggata acgtaagttg agggtgcagc ggcataacat
     6781 tggcagaaca acatctttaa cctttcacac cacctgccgt caggccgttg accagccagc
     6841 gttgagccag caagaagacg atggtgatcg gtaatgcaga catcacggca gcggcggcaa
     6901 agtcacccca caggtagttt tgcgggttga ggtattgctg catccccacg gccagggtgt
     6961 agctgtttac gtcacgcagt aacagcgacg cgaccggaac ttcagtaatg gcagcgataa
     7021 acgacaggat gaataccacc gccagaatcg gtactgacag cggcaacagg acaaggcgga
     7081 aggcctgcca cggtgtcgca ccatccagcg cagccgcttc ttccagcgaa ctgtcgatgg
     7141 tttcgaaata gcctttgatg gtccagacat gcagcgcaat cccacccaga tacgcgaaaa
     7201 ttacgccgcc gtgagtattc aggccaatga atggaatgta ctcacccaga cgatcaaaca
     7261 acgcatacaa cgcgaccagt gaaagtactg ccgggaacat ctggaaaatc agcattcctt
     7321 tcagcagcgt cgctttgcct ggaaagcgca tacgggcgaa agcgtaggcg caggtggtgg
     7381 agagcgccac aatgccaatc gcggaaatcc cagcgacctt taccgagttc cacagccaca
     7441 gcagtaccgg gaatggcggt ggcgtaatgc gaccatcagc ctgttcaacg ctaaaaccta
     7501 acgccagttt ccagtgatcc caggagattt gctccgggat caggctaccg gtcgcgaagt
     7561 ttccctgacg cagcgagata gcgacgacca tcagcagcgg gaacataatc gccgcgataa
     7621 aaagtagcag tagcaggtga gtaataaaca aacgtgcttt ttgcgatttc ggttggacca
     7681 ttgccatttt tgttatctcc cttaatcaaa cttcattcgc gtggctttca ggttcactat
     7741 cgccagagca cccaccagca ggaagatcag cgtggcaatt gctgccgcca gaccgaagtc
     7801 ctgacccccg ccgccttcaa aagcgatgcg gtaagtgtag ttaacaagca ggtcggtata
     7861 accggctggc gtggtcgtgc caagacgatc cgggccgccg ttggttaaca gctgaatcag
     7921 cacgaagttg ttaaagttaa aggcgaagct ggcgatcatc agcggcgtca gcggtttaat
     7981 cagcagcggc agcgtaatct taaagaagtt ctggaacgga cctgcgccat ccattgctga
     8041 ggcttcatac aaatcgtccg gaatcgcttt cagcaagccc atgcagagga tcatcatgta
     8101 cggataaccc agccaggtat tgacgataat cagcatcgtt cgggcagtgg tcggatcgct
     8161 gaaccaggcg ggcttcacgc caaacagcgc gctcaacatc atgttgattt caccgaagct
     8221 ctggttaaac aaccctttga aaatcaagat tgaaatgaat gatggcaccg cgtagggcag
     8281 aatcagcagg acgcgatata ccgctttgcc acgcaacgct tcccactgca ccagacacgc
     8341 cagaaccatg ccgaccgcca ccgttaaaaa gacggtgatc agtgaaaaca ccacggtcca
     8401 gacgaaaatg gcgaggaacg gtttctgaat gccttcgtcg gtaaagacgc gggtaaagtt
     8461 tttccagccg gtggtcacgg tgtaaccggg gcttagcttt tcatcccccc agttgccgtc
     8521 ggcggtaatg gactggtaaa agccaatttg gttattcgga cgatatttca cgccactctg
     8581 attattcgtc aacgtgccgt taccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg
     8641 gcgcagggag ctcatcatca ctttgttgcc atccggcaga atggcggtaa tgtcactcag
     8701 cgcctgacga ttctgggtaa tcacgcgcag attcgcgcgt tcgccttcgg gctgggcggt
     8761 cgtttctttc agttgcagtt tttgctcgcc gccaaattta aacgcgtcgg agaggtaatt
     8821 tttgccggtt tcgccgtcgc tgagcgccag ttgccactca tcgcccgccg ggtaaagacc
     8881 aaagttatag gttttgcctg cttgccagga gcgatctaac aacacttcct gcgcacgttc
     8941 aaaagtcagc tggttagtgc tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag
     9001 agggaagagg acgaataatc ccattccagc cattcccggg taaacatagc gccaggcgta
     9061 ggctttacga ttggcgaaaa tatacagccc cgctgaactc aatatcagcg tggtaatggc
     9121 gaacaggtat tccccttgtg cgtacattaa aacaacaagg taacccacca gcaggccgag
     9181 cagacctagc actgaccatt tcagcgcgtc gctttgccac caatgtttct ttttaatgac
     9241 atccatgggg ttcttcctca ttccaggacg gataaggctt tcacgcctta tccgacaaca
     9301 actgcctgat gcgacactga tgcgtcttat caggcctaca tgcgtttcag ttttgtaggc
     9361 cggacaaggc gttcacgccg catccggcat ttcacagcat tacttggtga tacgagtctg
     9421 cgcgtctttc agggcttcat cgacagtctg acgaccactg gcggcgttga tcaccgcagt
     9481 acgcacggca taccagaaag cggacatctg cgggatgttc ggcatgattt cacctttctg
     9541 ggcgttttcc atggtggcgg caatacgtgg atctttcgcc aactcttcct cgtaagactt
     9601 cagcgctacg gcacccagcg gtttgtcttt attaaccgct tccagacctt cgtcagtcag
     9661 cagatagttt tcgaggaact ctttcgccag ctctttgttc ggactggcgg cgttaatacc
     9721 ggcgctcagc acgccaacga acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt
     9781 tacaccataa ttcactttgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt
     9841 catcgctgtt tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt
     9901 catgtgtttg tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt
     9961 atccacgccc acgtctttaa tgtcgtactt gccgttttca tacttgaacg cataaccccc
    10021 gtcagcagca atcagcggcc aggtgaagta cggttcttgc aggttgaaca tcagcgcgct
    10081 cttacctttc gctttcagtt ctttatccag cgccgggatc tcttcccagg tttttggcgg
    10141 gttcggcagc agatctttgt tataaatcag cgataacgct tcaacagcga tcgggtaagc
    10201 aatcagcttg ccgttgtaac gtacggcatc ccaggtaaac ggatacagct tgtcctggaa
    10261 cgctttgtcc ggggtgattt cagccaacag gccagattga gcgtagccac caaaacggtc
    10321 gtgcgcccag aagataatgt cagggccatc gccagttgcc gcaacctgag ggaatttctc
    10381 ttccagttta tccggatgct caacggtgac tttaattccg gtatctttct cgaatttctt
    10441 accgacttca gcgagaccgt tatagccttt atcgccgtta atccagatta ccagtttacc
    10501 ttcttcgatt ttggcgagag ccgaggcgga aaacatcatc gtcgttaatg cggataatgc
    10561 gaggatgcgt gcacctgttt ttattttcat aatctatggt ccttgttggt gaagtgctcg
    10621 tgaaaacacc taaacggact ctagtttctt tatacggcaa cctctttcca tcctccttgc
    10681 ccctacgccc caccgttgct ttgtgtgatc tctgttacag aattggcggt aatgtggaga
    10741 tgcgcacata aaatcgccac gatttttgca agcaacatca cgaaattcct tacatgacct
    10801 cggtttagtt cacagaagcc gtgttctcat cctcccgcct cctcccccat aaaaaagcca
    10861 gggggtggag gatttaagcc atctcctgat gacgcatagt cagcccatca tgaatgttgc
    10921 tgtcgatgac aggttgttac aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt
    10981 aacgaaagcc tggggcgagg tcgtggtatc gaaagatatc aatctcgata tccatgaagg
    11041 cgaattcgtg gtgtttgtcg gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat
    11101 tgccgggctt gagacgatca ccagcggcga cctgttcatc ggtgagaaac ggatgaatga
    11161 cactccgcca gcagaacgcg gcgttggtat ggtgtttcag tcttacgcgc tctatcccca
    11221 cctgtcagta gcagaaaata tgtcatttgg cctgaaactg gcaggcgcaa aaaaagaggt
    11281 gattaaccaa cgcgttaacc aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg
    11341 caaaccgaaa gcgctctctg gcggtcagcg tcagcgtgtg gcgattggcc gtacgctggt
    11401 ggccgagcca agcgtatttt tgctcgatga accgctctcc aacctcgatg ctgcactgcg
    11461 tgtgcaaatg cgtatcgaaa tctcccgtct gcataaacgc ctgggccgca caatgattta
    11521 cgtcacccac gatcaggtcg aagcgatgac gctggccgac aaaatcgtgg tgctggacgc
    11581 cggtcgcgtg gcgcaggttg ggaaaccgct ggagctgtac cactatccgg cagaccgttt
    11641 tgtcgccgga tttatcggtt cgccaaagat gaacttcctg ccggtaaaag tgaccgccac
    11701 cgcaatcgat caagtgcagg tggagctgcc gatgccaaat cgtcagcaag tctggctgcc
    11761 agttgaaagc cgtgatgtcc aggttggagc caatatgtcg ctgggtattc gcccggaaca
    11821 tctactgccg agtgatatcg ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca
    11881 actcggcaac gaaacccaaa tccatatcca gatcccttcc attcgtcaaa acctggtgta
    11941 ccgccagaac gacgtggtgt tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc
    12001 agagcgttgc catctgttcc gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc
    12061 gggcgtttaa gcaccccaca aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa
    12121 agcaatgact caggagatag aatgatgatt actctgcgca aacttcctct ggcggttgcc
    12181 gtcgcagcgg gcgtaatgtc tgctcaggca atggctgttg atttccacgg ctatgcacgt
    12241 tccggtattg gctggacagg tagcggcggt gaacaacagt gtttccagac taccggtgct
    12301 caaagtaaat accgtcttgg caacgaatgt gaaacttatg ctgaattaaa attgggtcag
    12361 gaagtgtgga aagagggcga taagagcttc tatttcgaca ctaacgtggc ctattccgtc
    12421 gcgcaacaga atgactggga agctaccgat ccggccttcc gtgaagcaaa cgtgcagggt
    12481 aaaaacctga tcgaatggct gccaggttcc accatctggg caggtaagcg cttctaccaa
    12541 cgtcatgacg ttcatatgat cgacttctac tactgggata tttctggtcc tggtgccggt
    12601 ctggaaaaca tcgatgttgg cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa
    12661 gctggtggtt cttcctcttt tgccagcaac aatatttatg actataccaa cgaaaccgcg
    12721 aacgacgttt tcgatgtgcg tttagcgcag atggaaatca acccgggcgg cacattagaa
    12781 ctgggtgtcg actacggtcg tgccaacctg cgtgataact atcgtctggt tgatggcgca
    12841 tcgaaagacg gctggttatt cactgctgaa catactcaga gtgtcctgaa gggctttaac
    12901 aagtttgttg ttcagtacgc tactgactcg atgacctcgc agggtaaagg tctgtcgcag
    12961 ggttctggcg tcgcgtttga taacgaaaaa tttgcctaca atatcaacaa caacggtcac
    13021 atgctgcgta tcctcgacca cggtgcgatc tccatgggcg acaactggga catgatgtac
    13081 gtgggtatgt accaggatat caactgggat aacgacaacg gcaccaagtg gtggaccgtt
    13141 ggtattcgcc cgatgtacaa gtggacgcca atcatgagca ccgtgatgga aatcggctac
    13201 gacaacgtcg aatcccagcg caccggcgac aagaacaatc agtacaaaat taccctcgca
    13261 caacaatggc aggctggcga cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc
    13321 tacgccaagt gggatgagaa atggggttat gactacaacg gcgatagcaa ggttaacccg
    13381 aactacggca aagccgttcc tgctgatttc aacggcggca gcttcggtcg tggcgacagc
    13441 gacgagtgga ccttcggtgc ccagatggaa atctggtggt aatagcaaaa cctgggccgg
    13501 ataaggcgtt tacgccgcat tcggcaacca acgcctgatg cgacgcttgc gcgtcttatc
    13561 agtatctaca acggctgtca aatgtaggcc ggataaggcg tttacgccgc atccggcata
    13621 aaaacaggtt gtcattatct gaaaggggcg aaagcccctc tgattatcgg gtttagcgcg
    13681 ctattgcctg gctaccgctg agctccagat tttgaggtga aaacaatgaa aatgaataaa
    13741 agtctcatcg ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg aatcagcctt
    13801 gccgatgtta actacgtacc gcaaaacacc agcgacgcgc cagccattcc atctgctgcg
    13861 ctgcaacaac tcacctggac accggtcgat caatctaaaa cccagaccac ccaactggcg
    13921 accggcggcc aacaactgaa cgttcccggc atcagtggtc cggttgctgc gtacagcgtc
    13981 ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa acaaaccagc
    14041 gtttttgcgc cgaacgtgct gattcttgat cagaacatga ccccatcagc cttcttcccc
    14101 agcagttatt tcacctacca ggaaccaggc gtgatgagtg cagatcggct ggaaggcgtt
    14161 atgcgcctga caccggcgtt ggggcagcaa aaactttatg ttctggtctt taccacggaa
    14221 aaagatctcc agcagacgac ccaactgctc gacccggcta aagcctatgc caagggcgtc
    14281 ggtaactcga tcccggatat ccccgatccg gttgctcgtc ataccaccga tggcttactg
    14341 aaactgaaag tgaaaacgaa ctccagctcc agcgtgttgg taggaccttt atttggttct
    14401 tccgctccag ctccggttac ggtaggtaac acggcggcac cagctgtggc tgcacccgct
    14461 ccggcaccgg tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta ttttaatacc
    14521 gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg cgttaaaact gcttgatgaa
    14581 gctgaacgcc tgggatcgac atctgcccgt tccaccttta tcagcagtgt aaaaggcaag
    14641 gggtaattac gccccacagt gctgattttg caacaactgg tgcgtctcct ggcgcacctt
    14701 tttttatgct tccttcctgg gatatgagcg attttttata gtaactcact tcttcttcac
    14761 taagaatatc cattatctca atgccttatc agagattctt ttcctttcgc cggtagtgtc
    14821 tggacattca ggctactttt ccaggttatt ttatttctgt tatgcagagg ttttatgata
    14881 agtcatatcc taaattctgg cggcaataac tctttgatga aacatgatgt ga
//
LOCUS       DAZFFI010000105        14733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFFI010000105 DAZFFI010000000
VERSION     DAZFFI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.105"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            87..5507
                     /locus_tag="ACHJ12_003847"
     CDS             87..5507
                     /locus_tag="ACHJ12_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO4991568.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMDISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEE
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5716..5982
                     /gene="hipB"
                     /locus_tag="ACHJ12_003848"
     CDS             5716..5982
                     /gene="hipB"
                     /locus_tag="ACHJ12_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO4991569.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5982..7304
                     /gene="hipA"
                     /locus_tag="ACHJ12_003849"
     CDS             5982..7304
                     /gene="hipA"
                     /locus_tag="ACHJ12_003849"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO4991570.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEAKLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7623..7825
                     /locus_tag="ACHJ12_003850"
                     /pseudo
     CDS             7623..7825
                     /locus_tag="ACHJ12_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151642.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8257..8820
                     /gene="fimA"
                     /locus_tag="ACHJ12_003851"
     CDS             8257..8820
                     /gene="fimA"
                     /locus_tag="ACHJ12_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4991571.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9181..9891
                     /locus_tag="ACHJ12_003852"
     CDS             9181..9891
                     /locus_tag="ACHJ12_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4991572.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9933..12584
                     /locus_tag="ACHJ12_003853"
     CDS             9933..12584
                     /locus_tag="ACHJ12_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4991573.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNELKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELNETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGQLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            12598..13128
                     /gene="ydeS"
                     /locus_tag="ACHJ12_003854"
     CDS             12598..13128
                     /gene="ydeS"
                     /locus_tag="ACHJ12_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991574.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            13141..13644
                     /gene="ydeR"
                     /locus_tag="ACHJ12_003855"
     CDS             13141..13644
                     /gene="ydeR"
                     /locus_tag="ACHJ12_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991575.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13704..14618
                     /gene="ydeQ"
                     /locus_tag="ACHJ12_003856"
     CDS             13704..14618
                     /gene="ydeQ"
                     /locus_tag="ACHJ12_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991576.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQSSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT         4116 a         3139 c         3688 g         3790 t
ORIGIN      
        1 acctctgaca atttaataag tttcattttt aatgtctgac gactcatctc tttcgtggct
       61 gtattgtttg tatatggagc gtttatatga atagaatcta tcgcgtgata tggaattgca
      121 ctctacaggt atttcaggcc tgctcggaat taactcgcag ggcaggtaaa acatcgacgg
      181 ttaatttgcg taaatcctct ggactgacaa cgaaattcag tagattgacg ctgggtgttt
      241 tgctggcact aagcggttca gcgtctggtg caagtctgga agttgataat gatcagatta
      301 ccaatattga tactgatgtt gcttatgatg cctacctggt tggctggtat ggcactggag
      361 tgcttaatat tttggctggc ggtaatgcct ccttaaccac tattactacc agcgtcattg
      421 gcgctaatga ggactcagag ggcaccgtta atgttttggg tggcacctgg cgattgtatg
      481 atagcggaaa taatgcaagg cctttaaatg tgggtcaatc cggaacgggg acgctgaata
      541 ttaaacagaa gggtcacgtc gatggaggct atttaagatt aggttcttcg acaggaggcg
      601 tcgggacggt caatgttgag ggagaggact ctgttctgac gaccgaatta ttcgaaatag
      661 ggagttacgg tacgggctca ttaaatatta cggataaggg ttacgtcacg agttcaatcg
      721 tcgccatttt aggctatcag gcgggcagta atggccaggt tgtcgttgaa aagggtggcg
      781 agtggctaat aaaaaataac gattcctcaa ttgaatttca aattggtaat caaggaacgg
      841 gggaagcgac tattcgcgag ggtggactga ttacggctga aaacacgatt atcggtggca
      901 atgctaccgg tatcggaacc ctgaatgtgc aggatcaaga ctctgtcatc acggtacgca
      961 gactctataa tggatatttc ggtaatggca cactcaatat ttccaataat ggactaatta
     1021 ataacaaaga atattcatta gtgggcgttc aggacggttc ccacggtgtc gtcaacgtga
     1081 ccgataaagg gcattggaat ttcctcggaa cgggcgaagc tttccgctat atctatatcg
     1141 gtgatgctgg cgacggtgaa cttaatgtct cgcgtgaagg taaagtagat tcgggaatta
     1201 tcactgcggg gatgaaagaa acaggcacag gcaacattac tgttaaggat aagaactccg
     1261 ttatcactaa tctcggaact aatcttggtt atgacggcca cggcgaaatg gatatcagta
     1321 atgaggggct tgttgtcagc aacggaggaa gctcactcgg ttatggagaa accggcgtcg
     1381 ggaatgtcag catcaccacg ggggggatgt gggaggtcaa taagaatgtc tataccacca
     1441 ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg cggtaagttc gtatcgcaaa
     1501 atattacttt tttgggcgat aaagcaagcg gtatcggcac actgaacctg atggatgcga
     1561 catcatcgtt cgatactgtg ggtatcaatg tcggtaattt tggtagcggt atcgtaaatg
     1621 tcagtaatgg tgccaccctt aattcaacgg gctatggatt tatcggagga aatgcctccg
     1681 gtaaggggat agttaatatt tcaacggaca gtctctggaa tttaaagaca tcatctacta
     1741 acgcacaatt gctacaggtc ggtgtattag gcaagggtga actgaatatt accaccggag
     1801 gtatagttaa agcgcgtgat acacagatag ctctcaatga caaaagtaag ggcgacgtga
     1861 gggtggatgg gcagaactct cttcttgaaa cattcaatat gtacgtaggg acatctggta
     1921 cgggtacgtt aaccctgacg aatagcggca cgctgaatgt cgaaggtgga gaagtttact
     1981 taggtgtttt tgaacctgct gtaggaacgc taaacattgg tgcagctcac ggtgaggcgg
     2041 cggcagatgc cgggtatata accaacgcga cgaaagtaga gtttggttct ggcgaaggtg
     2101 tttttgtctt taatcatact aataacagtg atgccggcta ccaggtcgat atgctgatta
     2161 caggtgacga taaagacgga aaagtgatcc atgatgcagg ccatacggtg ttcaatgcag
     2221 ggaatactta tagcggtaaa acgctggtca atgacggcct cctgaccatt gcgtctcata
     2281 cggcagatgg ggtaacgggc atgggttcga gtgaagtcac cattgcaagc cccggtacgc
     2341 tcgacattct cgcatcaacg aacagtgcag gagattacac gctgaccaat gcgctcaaag
     2401 gcgatggctt gatgcgagtg cagctgtcat cctacgacaa gatgtttggc tttacccatg
     2461 caacagggac tgaattcgcc ggtgttgccc aactgaaaga cagtaccttc actctggaac
     2521 gcgacaacac cgctgcgctt actcacgcga tgttgcagtc tgacagtgaa aataccacat
     2581 cggtaaaagt gggagagcaa tccattggtg gactggccat gaatggcggt acgctcattt
     2641 tcgatacgga tattcctgct gcgacgcttg cagagggata tatcagcgtc gatacgctgg
     2701 ttgtcggcgc gggtgactac acctggaaag gccgtaacta tcaggtaaac gggacgggcg
     2761 acgtgcttat cgacgtgcct aaaccgtgga atgatcccat ggcgaataac cctctgacga
     2821 cgctcaattt gctggaacac gacgatagcc atgtcggcgt tcaactggtg aaggcgcaaa
     2881 cggttattgg gtcgggtggc tcattaacgt tacgtgattt acagggcgac gaggtggaag
     2941 cggacaaaac gttacacatt gcgcaaaacg gaacggtggt cgccgagggt gattatggat
     3001 tccgcctcac gaccgcacca ggtgatggtt tgtacgttaa ctatgggctg aaagcgctga
     3061 acatccatgg tgggcaaaag ctgacgttag ccgaacatgg cggagcctat ggcgcaacgg
     3121 ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc aatcaatacg gtgcgacagg
     3181 tttcgctttc caacggtcag aacgactatc agggggcaac ctacgttcag atggggacat
     3241 tacgtaccga tgcggatggt gcacttggca acacccggga actgaacatc agcaacgcgg
     3301 ccatcgtcga tcttaatgga tcgacgcaga cggtagagac attcaccggg cagatgggtt
     3361 cgactgtttt gttcaaagag ggggcgctga cggtaaataa aggtgggatc agtcagggtg
     3421 aactgacagg tggcggaaac ctgaatgtta cagggggaac gctggctatc gaggggctta
     3481 atgcacgcta caatgcgtta accagcatta gcccaaatgc ggaagtcagc ctcgataata
     3541 ctcaggggtt aggcagagga aatattgcca atgacggtct gttaacgcta aaaaacgtga
     3601 ctggcgaact gcgtaatagc ataagcggga agggtatcgt gagcgcaacc gccaggacag
     3661 atgtagagct ggatggcgat aatagccgct ttgtggggca attcaacatt gatacaggca
     3721 gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc ttccgttatc aataatggcc
     3781 tgctcaccat ctccactgag cgtagctggg cgatgacgca cagtatcagc ggtagcggtg
     3841 atgtgacaaa actgggtacc gggatcctga ctcttaacaa cgattccgcg gcgtatcagg
     3901 gtacgacgga tatcgtgggg ggggaaattg ctttcggttc cgactctgcc attaatatgg
     3961 caagtcaaca cattaatatc cataacagcg gtgtgatgtc gggaaatgtc accactgcag
     4021 gtgatgtgaa cgttatgcct ggggggacac tgcgtgtcgc taaaaccaca atcggcggca
     4081 acctggagaa tggcggcacg gttcaaatga atagcgaagg ggggaaaccg gggaatgtac
     4141 tgaccgttaa cggcaactat accggaaaca atggcctgat gacgttcaac gcgacgctgg
     4201 gcggcgataa ttcgcccacc gataaaatga acgtgaaagg cgatacccaa gggaacactc
     4261 gcgttcgggt tgataacatt ggcggcgtcg gtgcacaaac ggtcaacggt attgaactca
     4321 ttgaggttgg cggtaattct gcaggtaact tcgcgctgac caccggaact gtcgaagctg
     4381 gggcttacgt ctacacgctg gctaaaggga aggggaatga cgagaaaaac tggtatctga
     4441 ccagtaaatg ggacggcgta acgccaccgg atacgcccga ccccatcaat aatccccctg
     4501 ttgtggatcc ggaaggccca tcagtttatc gcccggaggc cggaagctat atcagcaaca
     4561 ttgccgcagc caactcgctg tttagccatc gtttacacga ccgtctgggt gagccgcagt
     4621 atatagattc actgcattct caggggtcgg caagcagtat gtggatgcgt catgtcggag
     4681 ggcacgaacg ttcaagggcc ggtgacggtc agctaaatac tcaggctaac cgctatgtat
     4741 tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc gcaggatcgc tggcatcttg
     4801 gcgtgatggc aggttacgct aatcagcaca gtaatactca gagtaatcgt gtgggttata
     4861 aatcggatgg gcgcatcagc ggttacagcg ctgggctgta cgcgacctgg tatcagaacg
     4921 atgcgaataa gaccggcgct tatgttgaca gctgggcgct gtataactgg tttgataaca
     4981 gcgtcagttc cgataaccgt tctgctgacg actatgattc tcgcggtgtg acggcctctg
     5041 ttgagggtgg gtataccttt gaagcgggaa catttagcgg cagcgaagag acgctgaata
     5101 cctggtacgt ccagccacag gcgcaaatca cctggatggg tgtgaaagat tccgaccata
     5161 cccggaaaga tggaacgcgc attgaaacgg aaggcgacgg aaatgtgcaa actcgacttg
     5221 gggtgaaaac ctacctgaac agccatcacc agcgtgacga tggtaaacag cgtgagttcc
     5281 agccttacat tgaagcgaat tggatcaaca acagcaaagt ctacgccgtg aagatgaatg
     5341 gtcaaaccgt aggccgtgaa ggtgcgcgta atctcggtga agtacgtacc ggggttgagg
     5401 cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta ggtgataaag
     5461 gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaacc gtataagccg
     5521 catgtcgaga tggcatgcgg cttaatattg ccgacttcaa aggctttaaa gcctcccttt
     5581 tatccgcgat cgcggatatc gcagcgttta tcccgtacag cggataagat gtgtttccag
     5641 attgacttta tcctcactaa aggataaaac tgataatatc cccttaagcg gataaatttg
     5701 ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat
     5761 gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt
     5821 ggcattaagc aggcgacaat ttccaatttc gaaaacaacc ctgacaatac cacgctcacg
     5881 acatttttta agattttaca gtcgcttgaa ctctcaatga cactatgcga cacgaaaaat
     5941 gcctcgccag aatcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt
     6001 ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta
     6061 agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat
     6121 tgcagagggg gaatatcacc tcagatgccg tatttaactt cttcgataac ctgttacccg
     6181 atagcccgat tgtacgtgac cggatcgtta aacgttatca tgctaaatcc agacaaccgt
     6241 ttgatttatt gtcagaaata gggcgagaca gcgttggtgc cgtaacgtta ttacccgaaa
     6301 atgaaaccat aacgcgcccg ataatggcat gggaaaagct tactgaagcc aagcttgaag
     6361 aagtattaac ggcttataaa gcagatatcc ctctgggcat gattagagaa gaaaatgact
     6421 ttcgcatctc ggttgctggc gcgcaggaga agacagcgct gcttagaata ggcaatgact
     6481 ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa
     6541 tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc
     6601 tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa
     6661 atgtgcgcgc gttagcggtc gaacgttttg acaggcgttg gaatgctgag cgaacggttt
     6721 tacttcgctt gccacaggag gatatgtgtc agacattcgg tttaccttca tcggtgaaat
     6781 atgaatcaga tggaggccca ggcatcgcgc ggatcatggc ttttttgatg gggtccagcg
     6841 aggcgctgaa agatcgctat gattttatga aattccaggt cttccagtgg ttgattggcg
     6901 caacggacgg tcatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac
     6961 tcacgccatt ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaatacaca
     7021 tcagcgatct caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg
     7081 ataaaattta tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc
     7141 agatgcatga aatcctaagt gactttgcca gaatgattcc ggcagcactg gataacgtga
     7201 agacttcatt accgacagat tttccggaga acgtggtgac ggcagttgaa accaatgtgt
     7261 tgaggttgca tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc
     7321 gttgttaatg agcacgacaa tcatgaccgc caacgactgg attcttcctt ggtttatact
     7381 ggtgcgcata atgtatatta tgttaaatca tgtctgagtg atgcgaagtg cttttctggt
     7441 agtcgttatt cgttcagggt tcaagaactt aagcctctcc tctttcataa ttggcctgtg
     7501 catggagaaa tagcctggtt attccctgcc ccatgctaag agcgcagtgt ctgtcgcata
     7561 gacactgcat acgaaaacac cagaggtgtg ctgttactta tttaaggacg gatcaagcgg
     7621 gaatgagtcc actccggtat cagaaatggc tacgtctcaa tgaagtcagg cgaccgatgc
     7681 tgaatgaaca ttacgatgtc accacagcag cctatgctgt cggttacgaa agctatccca
     7741 tttcagtcgg gaatattcgc ggatgtttgg agagtcaccc aagagagata ttaccaggtt
     7801 aagaaagtct gtcggtcagc tttaataacc cctgcactta tctgtttaga gcaattcggt
     7861 gttagtttca gcaagcaaac attaaccata gctaatgatt tatagccata ttaaccattg
     7921 gtagcccttg acctttcctg acacttccct tgttcgcaat aatattctgt ttagtcacgg
     7981 tttataaata acatgcataa tgaaatcgtc atgttattta gcgacataaa atgtttttgc
     8041 aaatactctc ttcaacaagt gcacaataaa cttgttgtga ttaggtgtcc ttgtgattta
     8101 aagatattat cgttcttcgt gaaatgtaat aaccctggaa actatattct cagggcaaca
     8161 accaatcaag aatgattctt gtttttaagc aataacagaa tgacttaatt gttaactctc
     8221 tttattcagc tacttaaata taaattttgg agaattatga aactcaaaca tgttggtatg
     8281 attgtcgttt ctgtgttggc gatgtcgtct gctgcggtaa gcgcagccga gggtgatgaa
     8341 tcagtaacga ccactgttaa tggcggtgtt attcatttta aaggtgaagt ggtaaatgcc
     8401 gcttgtgcaa ttgattcaga atcaatgaac caaacggttg agctgggtca ggttcgttct
     8461 tctcgcctgg ctaaagcggg tgacctcagc tccgccgttg gcttcaatat caagctgaat
     8521 gattgtgata ccaatgtttc cagtaatgca gctgttgcat tcctgggtac tactgtcacc
     8581 agtaatgacg atacgttagc gctgcagagt tcagcggcag gctctgccca aaatgtcggt
     8641 attcaaattt tggaccgtac gggtgaggta ttaatacttg atggggccac ttttagtgct
     8701 aaaaccgact tgattgatgg cacgaatata ctgccattcc aggctcgtta tattgctctc
     8761 gggcagtccg tagctggtac tgcaaacgct gatgcgacct tcaaagttca atatctataa
     8821 ttcaatctta aggtggctat acgtcatctg aaattgaaac agggtgtgtg tggcatggct
     8881 acgcacaccc gagcgattta ccaatattca tctcattttt aatgagaaat ggtttgtatt
     8941 tggagcgcta tgaaaaatca cataatatct aaactatcaa taaacaacag tagttaaact
     9001 tactgttatt taaaatattt actttgtagc aaggttttcc tattaaaaga gaaccaacat
     9061 ttattacaaa cgcattaagt ctggaaaccc ggcgactcgc caatgtatta gcaacattaa
     9121 tcgattgtct gatgtaacgg ttttcccgct tatcctctta acacatggag aatgcatcag
     9181 atgcaaacta cgcgtacccc ctactctatt tcctttatgg cgacagtgtt acttttattg
     9241 ctctttgctt gccatagcac cgttgccaac gcagctgtcg cactgggtgc gacgcgggta
     9301 atttatccgg caaaccagaa acaggtttta ttgcctgtca cgaataatga tcctgccagt
     9361 gtctatctca ttcagtcatg gatagaaaat gcaggtgacc aaaaagacac ccagtttgtc
     9421 attaccccac cgcttttttc catgcaaggt aaaaaagaga atacgctgcg gataatcaac
     9481 gctactaatc atcagttacc aggcgaccgt gaaagcctgt tctgggtgaa tgttaaagct
     9541 attcctgcta tggaaaaaga ccagaagaat gagaatacat tacagctggc aattattagc
     9601 cgcataaaaa tgttttatcg ccccacgaac ctggcaatgg cacctgaaga agcgcctgca
     9661 atgcttcggt ttcgtcgcag cggtagcaaa ctcacgctta ttaatccaac gccctatttc
     9721 attaccgtaa caaatatgaa agcaggaaat agcaatctcc caaacaccat ggtgccgcca
     9781 aaaggtgaag tatcggtgga tatcacgcat gctgcaaccg gggatatcag ttttcagacg
     9841 atcaatgatt acggtgcatt aactccgcgt attaaggcaa ccatgcagta agccagtgac
     9901 ctaattaata ataaactcaa tcggaattaa taatgacagc ctttcgtgcc gcatttaagg
     9961 catatcgtat gcatcaggtg ttaataatgc ctcgctttgc acggcttacc attgcccttg
    10021 gcttagcaac tgcggtattt cccgttgacg cagagtatta ttttaatcca cggtttttaa
    10081 gcaatgatct tgctgaatct gtcgatttat ccgcctttac taagggacgc gaggcaccac
    10141 ctggtacata tcgggtcgac atttatttga acgacgaatt tatgactagt cgggatatta
    10201 cttttattgc agatgataat aacgcagaac tcatcccatg cctgagtaca gatcttcttg
    10261 tcagtcttgg tataaaaaaa atagcgttat tagacaataa agaacattcg gcagaaaaac
    10321 atgtgccgga caacagcgca tgcacaccac ttcaggatcg tttggccgac gcatcaactg
    10381 agtttgatgt tggtcagcaa catttgtcac tctccgttcc gcaaatttat gttggcagaa
    10441 tggctcgcgg ctatgtttct cctgatctgt gggaagaagg aataaatgct gggcttctaa
    10501 actatagttt taatggtaat tctattaata atcgtagtaa ccataatgca ggaaaatcca
    10561 actatgcata tttgaattta cagagtggca tcaacattgg tagttggcga ctgcgcgata
    10621 actcaacgtg gagttattac agtgggagta gcaattcatc tgacagcaat aaatggcagc
    10681 atatcaatac gtcggctgaa cgtgacatta ttcccttacg ctcacggtta acggtaggtg
    10741 atagttatac cgatggcgat atttttgata gtgtgaactt tcgtggcctc aaaataaatt
    10801 caacagaagc gatgttgccc gatagccaac atggtttcgc tccggtgatt catggtattg
    10861 cccgtggaac cgcacaagtg agtgtaaaac agaacggata cgatgtttat cagactactg
    10921 ttccacccgg cccttttact attgatgata tcaactctgc ggccaatggt ggtgatctgc
    10981 aagtaaccat aaaagaggca gacggcagta ttcagacatt atatgttcct tattcgtctg
    11041 ttccggttct ccaacgtgct ggatatacgc gttatgcgct tgccatgggg gaatatcgta
    11101 gtggaaataa cctgcaaagc tctcccaagt tcatacaagg tagcttgatg catggtctgg
    11161 aaggaaactg gacaccttat ggcggaatgc aaattgcaga agattatcag gccttcaacc
    11221 ttggtattgg gaaagattta ggactttttg gtgccttttc tttcgatatc acgcaggcca
    11281 atacgacact tgcagatggc acccgtcaca gtgggcaatc ggttaaatcc gtctacagca
    11341 aatccttcta ccagacagga accaatatcc aggtcgcagg atatcgctat tctacgcaag
    11401 gtttttataa cttatccgac agtgcctaca gtcgaatgag tggttacacc gtcaagcctc
    11461 ctaccggaga caccaatgag cagacacaat ttattgatta ttttaatctg ttctacagta
    11521 agcgtggtca ggaacaaata agcatctctc agcagcttgg aaattacggt acgacatttt
    11581 tcagtgccag tcgccaaagt tactggaaca cgtcacgcag cgaccagcaa atatcatttg
    11641 gattaaatgt gccgtttggt gatattacga cttcgctgaa ttacagctat tccaataata
    11701 tatggcaaaa cgatcgggat catttactcg cttttacgct taatgttccc ttcagtcatt
    11761 ggatgcgtac agacagtcag tcggcatttc gtaattcaaa cgccagttac agtatgtcaa
    11821 acgaattgaa aggcggcatg accaatctat cgggggttta tggcactctg ctgccggata
    11881 ataacctgaa ttatagcgtt caggtcggta acacccacgg aggtaataca tcgtctggca
    11941 ccagtggtta cagttctctt aattatcgtg gagcttatgg taatactaat gtcggttaca
    12001 gtcggagtgg tgacagcagc cagatttatt acggaatgag tggtgggatt attgctcatg
    12061 ctgatggcat cacctttgga cagccgctgg gcgacacaat ggttctggtt aaggctcctg
    12121 gtgctgataa tgtcaaaata gagaaccaga ccggaattca taccgactgg cgtggctatg
    12181 ccatattacc atttgcgaca gaatatagag aaaaccgtgt tgctcttaac gcgaattccc
    12241 ttgcagataa tgttgaactg aatgaaaccg tggtcactgt catcccaact cacggtgcta
    12301 ttgccagagc aacatttaat gcacaaatcg gcgggaaagt attaatgacg ttgaagtacg
    12361 gtaataagag cgttccattc ggtgcaattg tcacacacgg agagaataaa aatggcagca
    12421 ttgtcgcgga aaatggtcag gtttatctga ctggacttcc acagtcaggg caattacagg
    12481 tttcatgggg caaagataaa aactcaaact gtattgtcga gtacaagctt cctgaagttt
    12541 ctcctggtac cttactgaac cagcagacag caatctgtcg ctaaggaaca atagattatg
    12601 aaatacaata acattatttt cctcggttta tgtctggggt taaccaccta ttctgcttta
    12661 tccgcagata gcgttattaa aattagcggg cgcgtcctcg attatggctg cacagtctca
    12721 tcggattcgc ttaattttac cgtagatctc caaaaaaaca gtgccagaca atttccaacg
    12781 accggtagca caagtccagc cgtccctttt cagattacgt taagtgaatg cagcaaaggg
    12841 acaacggggg ttcgggttgc atttaacggt attgaggatg cagaaaataa tactttgttg
    12901 aaactggatg aaggaagcaa tacggcctcc ggtttgggta tagaaatatt ggacgcaaat
    12961 atgcgtccgg tgaaactgaa tgaccttcat gccgggatgc agtggatccc actggtacca
    13021 gaacagaaca atattttgcc ttactccgct cgtctgaagt caactcagaa gtccgtcaat
    13081 ccgggactgg tgagggcttc ggcaaccttt acccttgaat ttcaataatc agagtcagat
    13141 atgaaaagac ttcacaagag gttcctgtta gctacgtttt gcgcgttatt cacagcaact
    13201 ctccaggccg ccgatgtcac tatcactgtt aatggtcggg tagtcgctaa accctgcact
    13261 attcaaacca aagaagctaa cgttaatctc ggggatcttt atacgcgcaa tctgcaacaa
    13321 cctggttctg catctggctg gcacaatatt actttgtcat taaccgattg tccggttgaa
    13381 acaagtgcag tgacggcaat cgtgacaggt tcaactgaca atacgggtta ttacaaaaat
    13441 gaaggtactg ccgaaaatat tcagatagag ctgagggatg accaggatgc gacgttaaaa
    13501 aatggcgata gcaaaacggt tattgttgat gagatcactc gtaatgcaca gtttccactt
    13561 aaggcaagag ctatcacggt gaatggaaac gcaagccagg gaacgatcga ggcgctaatc
    13621 aatgtgatct acacctggca ataacgtcga ttcgattttc ttagccttct ttttaaatca
    13681 attttttaac cttaggatat atcatgggta aaacaatcag tataaaggtc ctgttcggta
    13741 tttatcttct attaatggca ggtaaagttt ttgccttcag ttgtaatgtc gatggcgggt
    13801 caagtattgg agctggtaca acctctgttt atgttaatct cgaccctgta atacagccgg
    13861 gccagaatct ggttgtagac ttgtctcagc atataagttg ctggaatgat tacggcggct
    13921 ggtacgacac tgatcatata aacctggtac aaagctcagc tttcgcaggc tctctacagt
    13981 catataaagg atcgctgtac tggaataatg tgacctaccc cttcccgttg acgacaaata
    14041 ccaacgtatt ggatattggt gataagacac cgatgccatt accgttaaaa ctctatatta
    14101 ccccagttgg ggcggccggt ggagttgtca taaaagcggg ggaagtcatt gcccgtattc
    14161 atatgtataa aattgcaaca ttaggaagcg gaaatccgcg taattttacg tggaatatta
    14221 tttcaaataa caatgttgtt atgccaacag gcggctgcac cgttgatagt cgtaatgtca
    14281 ccgtggatct cccggacttt cctggaagcg cagaaatacc ccttggcgta tattgctcaa
    14341 gcgaacaaaa gctctcattt tatctttcgg gtgccaccac tgacagtagc cgacaagtgt
    14401 ttgctaacac agcacctgat gcaacaaaag catcgggagt gggtgtcact ctaatgcgta
    14461 atggaaaaat actggctacg ggagaaaatg tctccctggg gacagtaaat aaaagcaaag
    14521 tacctctggg tttaagcgca acctatggac aaacaggaaa caaggttgca gccggaaccg
    14581 tccagtcagt cattggtgtc acttttatct atgaataatt gtgattttga ccttagtcta
    14641 tgcgctccat tcacgaaaat tgcgcggata attattttgt gaaggctatt agcctacacc
    14701 tgtaagaaaa tccgcgcaca attgaagcca gag
//
LOCUS       DAZFFI010000106        14550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFFI010000106 DAZFFI010000000
VERSION     DAZFFI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.106"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..665
                     /locus_tag="ACHJ12_003857"
     CDS             99..665
                     /locus_tag="ACHJ12_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991577.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            716..3175
                     /gene="ybgQ"
                     /locus_tag="ACHJ12_003858"
     CDS             716..3175
                     /gene="ybgQ"
                     /locus_tag="ACHJ12_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4991578.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPVISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEKGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3190..3921
                     /gene="ybgP"
                     /locus_tag="ACHJ12_003859"
     CDS             3190..3921
                     /gene="ybgP"
                     /locus_tag="ACHJ12_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4991579.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPTALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3918..4979
                     /gene="ybgO"
                     /locus_tag="ACHJ12_003860"
     CDS             3918..4979
                     /gene="ybgO"
                     /locus_tag="ACHJ12_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991580.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5131..6177
                     /gene="abrB"
                     /locus_tag="ACHJ12_003861"
     CDS             5131..6177
                     /gene="abrB"
                     /locus_tag="ACHJ12_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4991581.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6174..6965)
                     /gene="nei"
                     /locus_tag="ACHJ12_003862"
     CDS             complement(6174..6965)
                     /gene="nei"
                     /locus_tag="ACHJ12_003862"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO4991582.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7001..7735)
                     /gene="pxpA"
                     /locus_tag="ACHJ12_003863"
     CDS             complement(7001..7735)
                     /gene="pxpA"
                     /locus_tag="ACHJ12_003863"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4991583.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7725..8657)
                     /gene="pxpC"
                     /locus_tag="ACHJ12_003864"
     CDS             complement(7725..8657)
                     /gene="pxpC"
                     /locus_tag="ACHJ12_003864"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4991584.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8651..9307)
                     /gene="pxpB"
                     /locus_tag="ACHJ12_003865"
     CDS             complement(8651..9307)
                     /gene="pxpB"
                     /locus_tag="ACHJ12_003865"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4991585.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9330..10073)
                     /gene="ybgI"
                     /locus_tag="ACHJ12_003866"
     CDS             complement(9330..10073)
                     /gene="ybgI"
                     /locus_tag="ACHJ12_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4991586.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLVADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10344..11825
                     /gene="dtpD"
                     /locus_tag="ACHJ12_003867"
     CDS             10344..11825
                     /gene="dtpD"
                     /locus_tag="ACHJ12_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4991587.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11867..13285)
                     /gene="phrB"
                     /locus_tag="ACHJ12_003868"
     CDS             complement(11867..13285)
                     /gene="phrB"
                     /locus_tag="ACHJ12_003868"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4991588.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGDYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13282..13791)
                     /gene="ybgA"
                     /locus_tag="ACHJ12_003869"
     CDS             complement(13282..13791)
                     /gene="ybgA"
                     /locus_tag="ACHJ12_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4991589.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13942..14550)
                     /locus_tag="ACHJ12_003870"
                     /pseudo
     CDS             complement(13942..14550)
                     /locus_tag="ACHJ12_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3531 a         3875 c         3519 g         3625 t
ORIGIN      
        1 attaataata tttttaagaa aaactaaaaa cattcgacaa tcaaataaga tattcttgca
       61 ccgatatact tttttctttt ctgttaaagg actccgttat gtttaaagga caaaaaacat
      121 tggccgcact ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat
      181 ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg
      241 atattgataa agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc
      301 acagcgatgc cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca
      361 acggttccgg tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca
      421 ccggcgcaga tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac
      481 gtttaatgaa taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc
      541 tggctccgac ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc
      601 aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata
      661 aataagtatt actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga
      721 caccgtgaat atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc
      781 attggcggtc gaattcaacc ttaatgttct cgataaatca atgcgcgacc gcattgatat
      841 ttcattatta aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt
      901 aaataataac caaatcagta acgggcaaaa gattaactgg cacaaaaatg acgataaaac
      961 cattccgtgc atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca
     1021 gtcgttacca ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa
     1081 tttcgatcaa gccaatcagc aactaaatat caccattccg caagcctggc tggcgtggca
     1141 ctcagaaaac tggaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga
     1201 ttacaacttg tttgccagca gctaccgccc acaggatggc agcagcagca ctaatcttaa
     1261 cgcctatgga acagccggaa tcaacacggg ggcatggcgc ttacgcagtg attatcagtt
     1321 gaatcagact gatagcgatg ataaccatga acagtcaggc gaaatatcac gtacctatct
     1381 ttttcgcccg ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg attttagttc
     1441 caatattttc gacggttttt cttataccgg cgcggcactg gcaagtgacg atcgaatgtt
     1501 gccatgggaa ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac
     1561 ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt
     1621 tatcattgac gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga
     1681 agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgtca
     1741 gggacaggtt cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac
     1801 tgaaaatgaa acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct
     1861 gtacggcggc ctgctgcttt ctggtgatga ctaccattct gccgcaatag gtatcgggca
     1921 aaatatgctg tggcttggcg cgctgtcgtt tgatgtcacg tgggccagta gtcactttga
     1981 tactcagcag gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc
     2041 caccaacagc acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta
     2101 cgccaactat cttgatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat
     2161 cagcttatct gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct
     2221 acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacag ccggttttaa
     2281 tgttgatatt ggtgactgga gagatatctc tatttcgacg tcattcaata caacccatta
     2341 cgaagataaa gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg
     2401 tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg
     2461 gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg
     2521 tccggacaat ggagcccagg tgagcggtaa ctatcagcat ctgagttcag cgggtgagtg
     2581 ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg
     2641 ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc
     2701 acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta
     2761 caccaaccat tttggcattg cggtggtgcc ggtgatttcc agttaccagc cttccaccgt
     2821 ggcggtgaac atgaatgact tacccgacgg agtaacagtt gcagaaaacg ttatcaaaga
     2881 aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt
     2941 taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca
     3001 ggatgacagc ggcattagcg tggggatggt tggcgagaaa ggacatgcct ggttaagcgg
     3061 agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt gctcgctcca
     3121 tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag
     3181 gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc
     3241 agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct
     3301 accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc
     3361 gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt
     3421 ctggaaccga aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta
     3481 ccgggtgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa
     3541 aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg
     3601 cgaccgactg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc
     3661 cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac
     3721 ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt
     3781 agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg
     3841 gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa
     3901 gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat
     3961 ttctgccgtt gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg
     4021 tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata
     4081 agatttggga atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca
     4141 attttgaaag tgagcatgtt tatgcctggg taaaccccta cccgggggtg caggatcgct
     4201 attatcaact cggcgtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc
     4261 gcatcgacac taaccagtgt attgacagta agaacattga catttatacc cctgagcaga
     4321 ttatcgcgat gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt
     4381 cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg
     4441 attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta
     4501 acgaagaccc tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcgcg
     4561 tgctggattg cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta
     4621 aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga
     4681 caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg
     4741 aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt
     4801 tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca
     4861 ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta
     4921 cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat
     4981 ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc
     5041 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
     5101 accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt
     5161 gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta
     5221 cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc
     5281 cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact
     5341 ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg
     5401 acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5461 aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa
     5521 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5581 gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac
     5641 cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca
     5701 gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg
     5761 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
     5821 ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa
     5881 atcttactgc gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg
     5941 gctatttgtg cgggtatggc gtgggggctg acccggttta tgcacattga ttttatgact
     6001 gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6061 aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg
     6121 acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc
     6181 tggcagcctg gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg
     6241 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6301 tgatgcttat tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt
     6361 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6421 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6481 gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggtga caataaccgt
     6541 tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa
     6601 aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc
     6661 agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct
     6721 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
     6781 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6841 ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt
     6901 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     6961 ggcatggtga tatccttttt tcattcttta ttaatcgggg ttatgctgcg acaacaatct
     7021 ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc
     7081 catgcaggca gactgtttgc gccgtgaccg ttgcccattc gccggtgata cttttgactc
     7141 tgccgtgttg caccatctct agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg
     7201 cgcctggctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca
     7261 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7321 gcccaacgag aaccaatgct ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7381 gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta
     7441 cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7501 aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac
     7561 tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg
     7621 tttgggcatc gcctgcatga aatccacaag caatattggc agaggaaacc agcgttaata
     7681 gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgt
     7741 agccgccacg ctaattgttc gaaataacgt tgttgatctt gccgcgcttt cagtgcctct
     7801 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7861 atatcagcct caatgataca ggcaatacgc gggtaaccac cggtggtctg tgcgtcgttc
     7921 atcaacacaa taggctgccc gttatgcggc acctgcacca cacccggtaa caaaccgtga
     7981 gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg
     8041 cggttacttt gcgggctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8101 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8161 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
     8221 tccttcagta aacggccttc cagcccgcca atccccacgt tgagatcggt gctgcatgac
     8281 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8341 tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag
     8401 acggcattat catccagccg cgcttcgcaa ccggcccctg tcagagcaaa ccacccatca
     8461 gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8521 ttacccacca gcaggttagc aatgcgcagc gcgggcatat ccagtgcgcc gcagtggctg
     8581 atacccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catgcccgca
     8641 cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac
     8701 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8761 actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga
     8821 cagaacctgc cggaacgagt aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8881 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     8941 cggaggagtg caattcaaca acctgttttt cgctcaaccc gcaatgcgcc gcgaccaccg
     9001 ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag
     9061 gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9121 gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca
     9181 cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg
     9241 tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9301 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
     9361 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accacgttca
     9421 gtggcatggt gacctgcagc ataaaaatgc aacccctgct cgcgggctga atgaatggtc
     9481 tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9541 aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg
     9601 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccagtccc
     9661 ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg
     9721 atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc
     9781 aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9841 attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca
     9901 tccgcgacca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca
     9961 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
    10021 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10081 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    10141 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10201 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    10261 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    10321 ttgccgtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact
    10381 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    10441 ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg
    10501 cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    10561 tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc
    10621 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg
    10681 gctatggtct gtttaaatcc aatgtcagtt gcctgctcgg cgagctgtat gagccaaccg
    10741 atccgcgtcg tgatggcggt ttctcgctga tgtatgcagc gggtaacgtg gggtctatta
    10801 tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc
    10861 tggcggcggt gggtatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca
    10921 cacatacccg cggcgttaat aaaaaagtac tgcgtgcaac aaactttctc ctgcctaact
    10981 ggggatggct gctggttctg ctggtggcaa cgcctgcgct gattaccgta ctgttctgga
    11041 aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa
    11101 aaatttatcg caaagcagaa aaccagaaac agcgtaagga gctggggctg attgtgacgc
    11161 tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggcagc tcgattagcc
    11221 tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg actgctatgt
    11281 tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta
    11341 aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggtctcg
    11401 gcctgatgag cgccgggttc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg
    11461 gtcactcttc tctgccactg atggtattag gcctggcggt gatgggcttt gcggaactgt
    11521 ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg
    11581 tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga
    11641 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca
    11701 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    11761 tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt
    11821 cataataggc cagcaccata cctgataaaa cgctcgctgc gttttatcag gctcctttcc
    11881 tcgcttcttc gtacgctgcc agcgttcgca gccttgcctc tttgtgatca actatcggtt
    11941 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt
    12001 tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc tcacggtcaa
    12061 atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca tcagtacccg
    12121 tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg
    12181 acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg
    12241 ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
    12301 catcaacaat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc
    12361 tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac aacgaggggt
    12421 aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac
    12481 cggccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
    12541 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
    12601 aatctcgttg ttgctcataa tctccggcac cgttctggca aaactggcgt aattgcgcaa
    12661 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
    12721 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggt gcagcgacgc
    12781 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
    12841 tgtacatctc gtgattgccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
    12901 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
    12961 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
    13021 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
    13081 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
    13141 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atataacgcc agcacgcgtg
    13201 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc
    13261 gaaaccagac cagatgggta atcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
    13321 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg
    13381 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
    13441 cgccgccaac tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
    13501 catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg
    13561 ttggtagtac tggttgtaaa aatcatccag attttgccac tggtgaatat cagccacgaa
    13621 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
    13681 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
    13741 aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgctgattca ttactgctcc
    13801 tgaacggttt acaggagtaa gtgtagacgt tgagaaatga tatttgtgaa gttaatcctg
    13861 ttgtagcagg acaaatttac gaaagcctgc tccccgcaag gactgacgtc aggtagtttc
    13921 taaccatagc tgccctttct attcaaacgt gaaatcaata agctcatcag ggacatcgca
    13981 aacaatatga agacgggttt cttctctgcc gtgactcttt tcactaattg cgtaactgtc
    14041 atgcttggga ttatttaatt ctttcagcgg aaatttttcc tcgaaggctt tatttagccg
    14101 cccctggttt ccttttacag cgaataaata atcacctccc tgtttttgta tcttctctgc
    14161 aatatctttc tggcaaccca tcgcatcggt tgtgatgatt tttcctttaa tatccagcat
    14221 gttaagaagt tcagggatag ctgtgatctc attggatttc ttatccgtct tgatctgccc
    14281 gatgaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg
    14341 actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt ctttatcatt
    14401 tgatgaatgg caatcacgca tccagttaat aaagcactcg tgaaattttg caggactgat
    14461 acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa aatcaccata
    14521 ttgcttcaag aaatcgagat gtgtttcccc
//
LOCUS       DAZFFI010000107        14445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFFI010000107 DAZFFI010000000
VERSION     DAZFFI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.107"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            35..391
                     /locus_tag="ACHJ12_003871"
     CDS             35..391
                     /locus_tag="ACHJ12_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4991590.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(442..1338)
                     /gene="yhaJ"
                     /locus_tag="ACHJ12_003872"
     CDS             complement(442..1338)
                     /gene="yhaJ"
                     /locus_tag="ACHJ12_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4991591.1"
                     /translation="MTKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1443..2144
                     /locus_tag="ACHJ12_003873"
     CDS             1443..2144
                     /locus_tag="ACHJ12_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4991592.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2167..2331
                     /gene="yhaL"
                     /locus_tag="ACHJ12_003874"
     CDS             2167..2331
                     /gene="yhaL"
                     /locus_tag="ACHJ12_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4991593.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2543..3853)
                     /gene="cyuA"
                     /locus_tag="ACHJ12_003875"
     CDS             complement(2543..3853)
                     /gene="cyuA"
                     /locus_tag="ACHJ12_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4991594.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3881..5212)
                     /locus_tag="ACHJ12_003876"
     CDS             complement(3881..5212)
                     /locus_tag="ACHJ12_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4991595.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5487..6851)
                     /gene="tdcG"
                     /locus_tag="ACHJ12_003877"
     CDS             complement(5487..6851)
                     /gene="tdcG"
                     /locus_tag="ACHJ12_003877"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4991596.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6923..7312)
                     /locus_tag="ACHJ12_003878"
     CDS             complement(6923..7312)
                     /locus_tag="ACHJ12_003878"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4991597.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7326..9620)
                     /gene="tdcE"
                     /locus_tag="ACHJ12_003879"
     CDS             complement(7326..9620)
                     /gene="tdcE"
                     /locus_tag="ACHJ12_003879"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4991598.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9654..10862)
                     /gene="tdcD"
                     /locus_tag="ACHJ12_003880"
     CDS             complement(9654..10862)
                     /gene="tdcD"
                     /locus_tag="ACHJ12_003880"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4991599.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10888..12219)
                     /gene="tdcC"
                     /locus_tag="ACHJ12_003881"
     CDS             complement(10888..12219)
                     /gene="tdcC"
                     /locus_tag="ACHJ12_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4991600.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12241..13230)
                     /gene="tdcB"
                     /locus_tag="ACHJ12_003882"
     CDS             complement(12241..13230)
                     /gene="tdcB"
                     /locus_tag="ACHJ12_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4991601.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13329..14267)
                     /gene="tdcA"
                     /locus_tag="ACHJ12_003883"
     CDS             complement(13329..14267)
                     /gene="tdcA"
                     /locus_tag="ACHJ12_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4991602.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLSADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3741 a         3875 c         3372 g         3457 t
ORIGIN      
        1 gactttcgtc acttttaatt aaagggatgt ttttatgcag tggtatttag ccgtattaaa
       61 aaattatgtt ggtttctctg gtcgcgcacg tcgtaaagag tactggatgt ttactctgat
      121 taacgccatc gtcggcgcta ttatcaatgt cattcaattg attttaggtt tagagtttcc
      181 attcctttct ctcatttacc tggcagcaac aatcattccg gtcattgctc tgtgtgttcg
      241 tcgtctgcac gatactgacc gttcaggtgc gtgggcgctt ctctatctgg taccgatcat
      301 tggctggtta gttctgtttg tatttgcctg tttagaaggc aattctggca gcaatcgtta
      361 cggaaacgat cctaagtttg gttcaaatta atcttagaat tggggcgaca tttcgcccct
      421 ttttattaat aataacaaat attattttcc gctaaaaagt ttgggaattt cccgcagaca
      481 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
      541 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
      601 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
      661 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
      721 agcggtatcc gctaccgcaa ttccacgata tttcacgcgc gtcacttcag ataacggttc
      781 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
      841 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacaatatc
      901 cgcccgcccc tgctccagtc gttcccacgc ccccgccagc acttcggtga tgattgccag
      961 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     1021 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgaggg cttccgcatc
     1081 ggtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     1141 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     1201 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgccgc
     1261 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     1321 tgccctttct ttggtcattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     1381 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     1441 ttatgattac tacccgaact gccagacagt gtggacaagc agactacgga tggttgcagg
     1501 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     1561 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     1621 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     1681 gcaatcatgt tcaggccagc gccggtgagg cgttgctgct ctctacccag ccgggtgtca
     1741 gctacagcga acacaatctc agcaaagaca aaccgttaac acgaatgcag ctttggctgg
     1801 acgcctgccc gcagcgagag aatccgctga ttcaaaaact ggcgcttaat atgggcaagc
     1861 agcaattaat cgcctcgcca gagggtacga tgggaagcct gcaattacgc cagcaagtgt
     1921 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     1981 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     2041 cggcgctgac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     2101 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     2161 atgatgatga gtaaaaaatc ggccaaaaag cgccagccag tgaagcccgt ggtggcgaaa
     2221 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagcc
     2281 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     2341 gccggatgcg gcgaaaacgc cttatccggc ctacaaaatc caacaacatt aacgaattaa
     2401 acaacccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     2461 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     2521 cacgataccc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     2581 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     2641 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     2701 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     2761 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatggttt
     2821 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     2881 cggcacacag cgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     2941 aaagcatcag cgcacgcgcc agccgttcat catccgctcc gaagtgttct gctaccacca
     3001 ccacaggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc atagccggaa
     3061 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     3121 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     3181 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     3241 gaataaagcg gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg
     3301 tcgttctgga aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct
     3361 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     3421 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     3481 tgcaaggttc ctggatctta acggagactt tccccgccgc cagcagtgct ttggcatcgg
     3541 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     3601 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     3661 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     3721 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     3781 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     3841 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg
     3901 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     3961 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     4021 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     4081 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     4141 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     4201 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
     4261 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
     4321 tattctgctc ataggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
     4381 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
     4441 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
     4501 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
     4561 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
     4621 acacgcccag cgccgcgacc accagcagct tggtcagcac catgccggtc gaaattttga
     4681 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
     4741 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
     4801 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
     4861 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
     4921 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
     4981 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
     5041 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
     5101 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
     5161 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
     5221 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
     5281 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
     5341 cttcacaatc aagactttga aataacggag atggataaga attttctact aaattaatcg
     5401 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
     5461 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
     5521 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
     5581 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
     5641 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
     5701 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
     5761 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ccgccattga acaggccacg
     5821 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
     5881 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
     5941 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
     6001 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
     6061 atccagtcga tgacattcat cggatcgtta gagatgttat cgctggaaac cagctgacga
     6121 cgcagcgcta cggcacgacg cggcacattg agcggaccag gcagcacgcc ttcagtgttc
     6181 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
     6241 tccgctttgc tgcgcagcgc tagctcgttg tgcatcatca ggccagatat agacaggccg
     6301 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
     6361 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
     6421 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
     6481 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
     6541 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
     6601 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
     6661 acatccgtgg catggccttt gcccgtcagt gacaacgacc catacagatc gaccacaata
     6721 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
     6781 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
     6841 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
     6901 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
     6961 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
     7021 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
     7081 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
     7141 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
     7201 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
     7261 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
     7321 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
     7381 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
     7441 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
     7501 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
     7561 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
     7621 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
     7681 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
     7741 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
     7801 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
     7861 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
     7921 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
     7981 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
     8041 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
     8101 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
     8161 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
     8221 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
     8281 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
     8341 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
     8401 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
     8461 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
     8521 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
     8581 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
     8641 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
     8701 tgtgcctgct gctcattgag tacgccagct ttaaagtcgc gctcaatgta gatatcgagg
     8761 aacgatgccg tgcggcccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
     8821 taagcgaagt agagccactg caccgcttcc tgcgcattct gcgccgggcg agagatatca
     8881 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
     8941 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
     9001 tcggcaaatt gcagttcgcg ttcacgtacc agataactga tgccatacag cgctacgcgg
     9061 cgatagtcac cgataatgcg cccacggcca tagccatctg gtaaaccggt cagcacgcca
     9121 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
     9181 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
     9241 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
     9301 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
     9361 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc attttcgata
     9421 cggatacctt ccattacttt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgagg
     9481 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
     9541 tcgtttttcc agtccgtacc tttaaagcca agccatgcgt cggcgtacag cttatcgctg
     9601 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
     9661 aattctgcgg gcgcgttaac tttgcctaaa tgaatggcat ccaaagcaat cattttttct
     9721 tcgttagtcg gaataacagc gcaaatgaca cgcgcatttt cactggaaac aattcgctca
     9781 ccacaggagt tagagcgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
     9841 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
     9901 attccatcca ggcgacgtaa tgaagctgcg tgtccggcaa tatgacgggc aattcggtga
     9961 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    10021 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    10081 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    10141 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    10201 gtatcaacac tctgaccgtt gcgaaccgcg cagattgacg cgccattgcc aagatgcgcc
    10261 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    10321 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    10381 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    10441 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattg
    10501 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    10561 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    10621 gccacactgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    10681 tcgtaactgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    10741 tccgagttaa taccgtcggc aatacctgac attaatactt cacagtcgct ggcatcgagc
    10801 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    10861 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    10921 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    10981 cggcgcttta cggatggcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    11041 gcccatggct tcaatcaggt caaggatgtt cgggttggcg taggcaacaa cccaggtgga
    11101 gcccatgatg aagatcatgc tgatagtgtt cagtttaccc agcgacactt tagttttgtc
    11161 gcctttataa ccaaacttca ggaccaggcc attcagacct tccagcgttc ccagatagtg
    11221 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    11281 aatcgcgaac gttgttttgg taccggtcat ggacgcaaag tggttagcca gataagaaag
    11341 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagcg taaacaggca
    11401 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    11461 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    11521 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    11581 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    11641 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    11701 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    11761 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    11821 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    11881 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    11941 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    12001 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    12061 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    12121 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    12181 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    12241 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    12301 aataatactg acggtttttc tgttttgaat atattggtct aatttaccgc ttaataatgc
    12361 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    12421 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    12481 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    12541 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    12601 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    12661 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    12721 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    12781 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    12841 attatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    12901 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    12961 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    13021 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    13081 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt ttgagcgagg
    13141 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tgtcatcaat
    13201 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    13261 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    13321 acaaataact aaccgacttc tattaattgc cttcgtctac acccattata agatgaatac
    13381 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    13441 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgattt
    13501 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc actgagaaca
    13561 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    13621 ctttgtaacg tagtaagaag ctcgctgtag taccccatat tggtttgtgg caacacccac
    13681 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    13741 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    13801 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    13861 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    13921 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    13981 accgcctcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    14041 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    14101 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    14161 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    14221 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    14281 aattacctca ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca
    14341 ctcacaaatt aactttcaat actttccatg gtatcgttac gcaaattaaa tacctttaaa
    14401 tatcaataag ttaaagtata aaaatcagca taaaccctga ttttt
//
LOCUS       DAZFFI010000108        14408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFFI010000108 DAZFFI010000000
VERSION     DAZFFI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.108"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1050)
                     /locus_tag="ACHJ12_003884"
     CDS             complement(<1..1050)
                     /locus_tag="ACHJ12_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991603.1"
                     /translation="MGNRLLRRAVSVAISGTLLGLGVGVSAAEDMAGLDVYSVVVEAD
                     GWTKEKSPVYGSIYKVYYNESSNWGTVRYIRDGKNETLFNFDNEGDIYVVNKEHSIRY
                     KVHEPVLKDFARMAEKLRTGDKVGHGLDEEEVRRITNKVNNLSNTIINPEVLKQSDFI
                     ASGPRPEGDRLVQASAAGELALAVGEGAAAEEKNSTAVGSWASANGRQSTALGSGGQA
                     YADGSTALGSYSHVDDSSVYGVALGDSSVVGENAPEGTALGSRAEVSHRNSVALGARA
                     RTTRDNEVYIGYAEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTN
                     TRLVVEAKKSREYTDS"
     gene            complement(1410..9791)
                     /locus_tag="ACHJ12_003885"
     CDS             complement(1410..9791)
                     /locus_tag="ACHJ12_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO4991604.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPHNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKVTGKAVSDGAKASDEWLTDQM
                     SPGAVRALNTPMSEGYDDSAVLLTKGVNLIGALVPDLVAGGVVRKVGQVALKRTLTEG
                     LEKKYIAAGVQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNA
                     DYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVN
                     AMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENMALRETAG
                     MDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQSAEEAAMRDAETVQQDDAAP
                     QPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQA
                     KRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQSQ
                     PENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS
                     DVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPMEQ
                     PAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRLP
                     APGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPENAAGRQ
                     GEILEGETVRRGLPSPDEQNATALVREGLPAPDITHNVRMPQPESLPRTVRDSLPELA
                     QQAEVRRQAGGNRDIPQPETIAPESETTVATDREATVRGGEVRGKKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYATQSLSKLFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAKV
                     WFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAH
                     YEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRV
                     FKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAAR
                     AFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRTL
                     KTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSD
                     LKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEI
                     IHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAH
                     MAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTP
                     REDGAGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVLR
                     HLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVML
                     LDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTEGGMSKANSLDKAGLTLYRKKKL
                     SRDNLLHRGLQLPKGEHSYRGSVDKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNTYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWRGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDVTPESLKYWIDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVE"
     gene            complement(9877..11139)
                     /locus_tag="ACHJ12_003886"
     CDS             complement(9877..11139)
                     /locus_tag="ACHJ12_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991605.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRLEYNDMLARDQSVMVALGKAVDAGDHDAASHLFGKLSDANPLKIMSTEGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKEVSKVVAQAIKDGRDAQSAKDEFLAAYDSL
                     PHQQKIQQRKTQQLLQKWQPWAAGDPVKQEFVKEAAANMPALLEPGREKEVEIYYQNH
                     LRDKKAEDEKALDYSASAAAEQIKGWIK"
     gene            complement(11150..11401)
                     /locus_tag="ACHJ12_003887"
     CDS             complement(11150..11401)
                     /locus_tag="ACHJ12_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO4991606.1"
                     /translation="MGYGLLDIANQSRREALQGISEADRRRTEMEASNKMMAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(11411..11857)
                     /locus_tag="ACHJ12_003888"
     CDS             complement(11411..11857)
                     /locus_tag="ACHJ12_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991607.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSGLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(11860..12510)
                     /locus_tag="ACHJ12_003889"
     CDS             complement(11860..12510)
                     /locus_tag="ACHJ12_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991608.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNFLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGGAKTPGTGANAIQHQAQNWRL"
     gene            complement(12604..12957)
                     /locus_tag="ACHJ12_003890"
     CDS             complement(12604..12957)
                     /locus_tag="ACHJ12_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991609.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACLKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(13093..14319)
                     /locus_tag="ACHJ12_003891"
     CDS             complement(13093..14319)
                     /locus_tag="ACHJ12_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4991610.1"
                     /translation="MKMTGKLLLAFVPALSLSVFSADAYAVDTSANSSGISGSPYNGI
                     TVDVLKSKQYADINSFWNDLGFPGKGYQDGSIVKVENGKITGPASVDIQNTAGKYLKY
                     SLYDAGKSGLTNTELLAPRSEIASYYDYANKVAYFVNTKTNQILFSADDKGLVTTVGT
                     DGKTYSRHVPEISEYIYEIKIKSVIPGSTINTVLFAEDVTDIHARLSALGSGETQVGG
                     GAITGGENATAVGYNATANYTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAA
                     TYGQLERYRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRK
                     RADAGIAGAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASW
                     DTQNGSGVAAGMSVGW"
BASE COUNT         3184 a         4292 c         3168 g         3764 t
ORIGIN      
        1 ggagtctgta tattcacgag actttttcgc ttcgactaca aggcgggtat tcgtatcctg
       61 agccagttgc tcaacactgt cttttacacg gtcaacctga cgaacggttg ctgcatctga
      121 gggacgagta ccgtcactca ggccaccaag cacacgagtt ttataagcct tgcccgcttc
      181 ttctgcgtag ccgatataaa cttcattatc acgggtagtg cgcgctctcg cccctaaagc
      241 aacactattt ctatgagaaa cttccgctct gcttcccagt gcagtgcctt cgggggcatt
      301 ttcacccaca acagagctgt cccctaatgc cacgccataa acggatgaat catccacatg
      361 agaataactt cccaacgcgg ttgaaccatc agcataagcc tgccccccac ttcctaatgc
      421 agtactctgt ctcccattag cactagccca tgaaccaaca gctgttgaat tcttttcctc
      481 cgccgccgca ccttctccaa cagccagagc gagttcgccc gccgcactag cctgaaccaa
      541 acggtcacct tccggacgtg gtcctgatgc gatgaaatcc gattgtttta atacttcagg
      601 attaataata gtatttgata gattattaac tttattcgta atgcgacgaa cttcttcctc
      661 atccagtccg tgacctactt tatcgccagt ccgaagcttt tctgccattc gggcaaaatc
      721 cttcaacaca ggctcatgca ccttatatct tattgagtgt tctttattca caacataaat
      781 atccccctca ttgtcaaagt taaataaagt ttcatttttc ccgtctctga tatagcgaac
      841 cgttccccag ttagaagatt cattgtaata gaccttatat atactaccgt acacaggtga
      901 tttttctttt gtccaaccat cagcttcaac aacgacgcta tatacatcca acccagccat
      961 atcttctgca gctgacacac ccacaccaag acccaacaac gttccactga tagcaacaga
     1021 aacagctctt cgcagcaagc ggttacccat gtatgattcc tcattattta tttgcacacg
     1081 tatcagtagc cacacctcag attcataaag aaaatctgat tagttttggc tgacatgagg
     1141 atattctgag aaaaatcata atatttaatt ttaaataacc aggaattatc ttaccctatt
     1201 cttggcgtat tcactcctga gacatggagc aacggaggga ctgaaataag aaaaatcccc
     1261 cccccataag gcatgatatt tcacatctgc ttacactgta atttctgatc aatatcacaa
     1321 cattatccga atttaactgt tcagttcatc tacaatattt acctgcgtga ctttatatca
     1381 gccccgcact tccgtacggg gcatgttctt tactccactc ccactttttt gttgtactcg
     1441 cggttaaacc gatccacggc aattttcata tcccgctcta ccgatttcac catcgccgac
     1501 tgttgcgcca gactgagagt actgtcggcg taaatggcat cacgctgttt acgcaaatcc
     1561 ttcagtctct tccgggtatc ctgcataagc ccgttcatcg acaattttcc gttgttctcg
     1621 tcaatgaacg ccgttctttc tgcaccggtc agactcttca gctctgcgtg atactgcgca
     1681 acctctgtca tccggtcgta catcttctgc tggtcagcat acggcatcac ctcacctgaa
     1741 attttcccca ggaagggcac ctgctgctcc ggtatatcaa taccattcag cgatttcacc
     1801 gccgcatccg tggttttgga aatgaagcgc cctgtcccac cagagatata gtcaatccag
     1861 tatttcagtg attccggcgt gacatccacc gcgccgggac ggtactggct gccacctgag
     1921 aacgcattca gccaggatgc aaacgccttg tacgcttctg gcgttgaacg tcttcccagt
     1981 tggctgtccg gttttggtgt accaaacggc atgttctcct ggtaaatctg cgcccccatg
     2041 aagttttcat tcatggcaag gttcgcaaac ggacgcagaa tggtcggcgc tgcatttttc
     2101 agcaatgccc cggacagtgt ttccgacgtt tcactaccaa tcgggctgaa tgcaccaagc
     2161 acaccaccaa caacattacc ggcagcacgg gacgcagtca ggtcacccgc cgccacacct
     2221 tcagcggtat gcccgagcag gaagaaaacg ttgtacccgt aaggcagagg aatactccag
     2281 tactctccgg ccttgccccc gaacatcgat ttcatgatga cgaggttacg ctctttcaca
     2341 tgagacggta ctttgtcata ccagttaacc ccgtcatcat cctcacccgc cacgctgcgg
     2401 ttaagcgagc caagcagata acccgctccc acagcggcaa gcgcgatttt ctgcggtaca
     2461 ttcagattct tccagcgaag gcgctccagt aaaggtccct caccattaag atgtccgagc
     2521 gttctcacca gatttgccgt tccctgaata ctggcgttgg cgaacatgta cagcgagttc
     2581 atcagcgccc cctgctcacc acgacggtta aagttcaccg tcatgttttt ggcaagagac
     2641 gccgcctgct ggcgtgacaa accggcatca cgggcgtgtt tataagctga cagacgcagt
     2701 gcgttttcaa ccgcgccgtt ggcatcctcg accaggttca ggaacgaatt ccatgcaccg
     2761 atactctggc ctctccatcc tcccttcgcc agtgagacaa ggcgatccat ttccttctgc
     2821 tggccttcaa ggtcacccat gttaaaccag ccggttttac ctccgtcctc aacaaactct
     2881 ttccacacct tctgccactg tgcacctttg cccgtgaggg ttttgccacg cagactggcg
     2941 tatacggctg acatggcaga acggctgtct ttcaccacag ccagtgcgga taagttatcc
     3001 agccctttca gtttgccgtc gctccttccc tgctcagcct tcaggttcat caccgccgtc
     3061 tgcacgtcac ggatgaagtt actgaccagg aattccgggt tatacgacgt gttcaccgtt
     3121 gccaggaagc ggttaacttt acccagcgta cggattacgg cgttactggt ttccggtccc
     3181 atattcttca tcgcacgcat caggcgcgga tcatggagtt tgatgtagta cgttttgccg
     3241 ttctttttgg tggtgaagta ccggtctgcc atcattgcca tcgccaccgg acgctcgaca
     3301 acttcgcgaa tggtttcacc agtttcctgg tccttgcgct ctgcaatcac acgcatggta
     3361 tccggtctgt catcagtgaa tacctgccag taatccttgt cgggattatc ctgcaccagt
     3421 ttcaggaagg cgttacccac ttcgtttttg cgatggcgga tcagcgattc gctcaagtcc
     3481 tgtatcgcct gagtggacgg agactgtgca cgggatgcac gccccatggc ctgcctgctt
     3541 tcacgtccgc cgatggtgaa gcccttacct gtacggggca gtgacaccac accgtcaaca
     3601 tcctggcctt tcaggggaac gtagtaacgg taggtgttct gccaggcatc caccacaccg
     3661 ctctcttcca gtccggcctc acggataagc tcacggcgac gggccagcat atcgtcaaca
     3721 atccctgcca gacggtcata ctgtgcctgt ttgccgctgt tacgcacacg ctgcatgatt
     3781 tccgccgcct ccgcgttggt catccccgaa ccgccgtccg gcattttcgg gttgattttc
     3841 gcgatatgcg cgttacgttc cggcgcgtga cgggcgtaga ggtactcatc cagatcggcc
     3901 tgcgcaatct tatagtctgc cagtaattta gccagcggct gaacatagcg ctccttcatc
     3961 acgttcaggt cgttttccgc cttcccgtgg aagagttctt ccgccatata agcgttgtta
     4021 ctgtcgtcta tttttccgcc agttttacgg atattctcct gaacagcttt cagcacctgg
     4081 aatttatcct gcatctggcg cacaaaacgc gatgcaattg tctcttccgg tgtcagactg
     4141 ctggtacggg agtaatacgg ccccttgcga atatcttcag gatagagtat tttatccaca
     4201 gagccgcgat aagaatgttc ccctttgggc aattgaagcc ctctgtgcag aaggttatcg
     4261 cggcttaatt ttttcttccg gtaaagcgtt aatccggctt tatccagact atttgcctta
     4321 ctcattcctc cttccgtacc gtaaacagaa gccaccttgt tgatttccag acgcgaccgg
     4381 acagccttca tgtgtaccgc tgacaccacc ggatcaccat ttttatccac ggcatcaagc
     4441 agcatcacaa ccgcatttct ttctgtcgcg gaacggtaaa ttgcatccgg atcgtgcatc
     4501 agttccggta gtctctcgat aacatccatc ggcaccacat gtttcacacc attggtggcc
     4561 ttccgcacag tatcgcggga aataaccagc ggcaaatccg gtgcaccaag gtgacgcaat
     4621 accggcggcg tacgcccgat gtttaccgtt aaatctgtgg tacgcagaga tttcatcatt
     4681 ctggcaaggt catcgcgata acgttcaccc tcaccttccg gcactttgaa cggatcaggt
     4741 ttaccactac gggagtactg agacgatgca cccgcgccat cctcacgcgg cgtgtaacct
     4801 tcccgcacac gctggcctaa cgtacggatg gtctcgcgaa caagtctgat atcgttcagt
     4861 tccgtcggtt tcagtaaccc cgtacgacgc agtacccctt tgaccagggc aacaacacgc
     4921 tcccatgccg ccacaaattt attcggctgc ttctctgcca tatgcgccag aaattcaccc
     4981 gcctgtactt ccggtgattc cttaccatag gacgcatcaa ccttacgcca ggcttcacgg
     5041 atggtggcgt tatcactgtc gcgggttttc agcacggttt tgataatcgt ctgatattcc
     5101 gctggcgtga caacgtgctc catggcatgg tgaattatct cgtgacgcag cttctcgcga
     5161 acggtccgcc cgtcagggat gttatccgcc accaggacaa tttcacgttt atccggacga
     5221 tagaacgcgt gcaccctgcc gtaaccgtcg aacgattcac ccgccagcgc ttcagcctct
     5281 ttctgtgact tcaccacacg gaccttcagg tcactgtcct taatgccgct catcacgcca
     5341 cgggcaaccg cttcaacctg cgggaccggg ctgcctttgg cttccgcact gcggttaaca
     5401 tccgaaatga gattgccttc aggtgtgcgg gtcacgccct tacgggaata aaacgcaacg
     5461 cccttgtccg tctcacgggt tttcagggtg cggaacaggt gatcgaatgc ctcacgaata
     5521 ccgccatcca gttccgcatt cgtcggataa gcgtaagtgt tatctgtgtt gtgctcaggt
     5581 gccttacgga tattgaccag ataatcattc tccacgccag ccatacgcgc cttatcctga
     5641 acataacgct caaaggcacg cgccgccatt tcaacatccg ttgaccagta cggttttgag
     5701 cgcacctcat cgagaagcgc tgaacgacgc ggcatgtcac tgtttttaat ggcctgaatc
     5761 acacctttaa aagcgtcgta aacctcctga cgtaccggat attcagcatc aacatacctg
     5821 ccgtctttaa atacgcgccc ggcacgctgt gcttccgtca tatagtcgcc acctgacgta
     5881 atcttcccgt cagtggaaac gtcataacga ccaaaataat tatccagaga atggaaccat
     5941 tcgtgcgcca gcgcacccgg tccgttacct tttgtcaggt tgattgccac ctcacccggc
     6001 tcatagtgtg ccgccgcctt acccttacca cgggcaccaa atgcaagccc aagacgaccg
     6061 ttcagggaaa gcgcttttgt cggtacgttc agcacgtcag ccaggtcatg cagcgagtca
     6121 taagcccggt tcaaatcagc ctgacggcgt ggaccttcca cgtagttacc aaactgcaca
     6181 ccacggaaac caaacgcatc actgaactgc tccggtgaaa catccccttt acggcgttct
     6241 ggtccggtac ggtcgcggtt ggtggcgtta cgctgctcct cacgcgaaat ctcccgcatc
     6301 tccttcacat gacgaacaag ctcatcacga tgtgaatcaa tgtacttacg cgcatcactg
     6361 gctgacttaa agccacctct cacccgcatt ttgtttttac cgtaagcgat aaaaatatcg
     6421 ccactgcggg tattccggta aacgtcaaag cggattttgt catccggtga cggtgctgtt
     6481 ttttcatcac ctttcgcctg tgatttttcc tcctgctctg caaaccagac tttcgccttt
     6541 gccagtaatt catccctgct ttccgagaaa aagaggttac tccccttatt gtccttattg
     6601 cgcagtgaat aaagtttctg tggtggatcg taacgcttcc ctcctgccgc ctgatacaca
     6661 cccgatacca cccgataagc agaagccctg tccatctgtg agggtggcag agtgcgtaac
     6721 agttgccagg tatccgcgta acgggagggc attctgcctt ccatccattc tgcgaggcgt
     6781 ttcgcgctga ccgttccgtt cagcatttcc gatacactgt gtcgtacttt ttttacgctc
     6841 tccccccagc ctgccgtatt gtgtttcgtc ttcgccggaa tatcgctacg atacagcgct
     6901 atcattgcca gagtgtcagc atcggcacct tcgttcgcca gttttgcgta gtccggtttc
     6961 gggaacagtt tgctcagcga ctgcgtggca taatccctgt cttccagcgt tttacccagt
     7021 gtttcagcaa gctgtgcata acggtgtttg gctgcccctt taatttcctc gccaaagtct
     7081 tcaatttttt tgcccctgac ttcacctccg cgcacggtag cttccctgtc agtagcgact
     7141 gttgtttcag attcaggtgc gattgtctca ggctgcggga tgtcacgatt tcctccggcc
     7201 tgtcgacgta cttctgcctg ctgtgcgagc tcaggcagtg agtcccgtac agtgcgggga
     7261 agtgattcag gctgaggcat acggacattg tgtgtaatgt ccggagcagg cagtccttca
     7321 cgtaccagcg ctgtggcgtt ctgctcatcc ggtgacggca gaccacgacg aactgtttcg
     7381 ccctccagta tttccccctg gcgaccagcg gcgttttccg gtgccggagc acgtcctttc
     7441 tggaaactct ggcccttaac ctcaccggtt gtggtaaaac gaccaccacg tccggcctga
     7501 ttctcatccg gcgtacgcgc cacctctccc ggtaacggat atccctgtcc gggatgaata
     7561 tcgccggggg cgggcagacg cggacgctca gttaactcct gtgctgtcgg accggcatca
     7621 ccttcagcca tctgtgaacg tacaagctcc tccgccgtcg gcgcgttgcc acgggcaagg
     7681 cgggcctgta cctccgtatc atcccccgtg aaaccacgga atcgcgggtc acgcatgaac
     7741 gcgggctgct ccatcggatc agcatcagca atacgctctc cgtcagccag ggtgttaatg
     7801 gtttccgttg ccacatcttc agagaacgca ggattatcca tttcaacggc atactgattc
     7861 tcgcctttct tcactacgga cggtttcagg ccggtggcgg cggcattacg gaaaacatca
     7921 ctccccatgg cgcttttctc atccgtgaaa taacggttgt ccgggcgcac cttttcaatg
     7981 cgttccgctg gtctggacgc accattcccg ttaatttcaa tgcggacttc attcgggtcc
     8041 cggaaccgca ccgtcgggta aatgctcccg ttaccatcct ccgcattttc aggctgtgac
     8101 tgtgcttccg gcgtcacttc ctgttgtggg cgaatgactt ctcgtattgc ctgaaactgt
     8161 tgttcttcag tctcggtgcg cccctctttc tggctgagtc tgcggtattc ctcaagcagt
     8221 tcagaacgcg gcttcgcctt cagctcattc atcacagcct gtcgcttcgc ctgttcgtcc
     8281 agttcattca acaactggct ggcggcttcc cggcgatgag ctgcggatgc gtcaccctct
     8341 gttgccatat ccgcatcagc atactgttcc agaagctgct cgcgattcat gccctgcatg
     8401 gattcacgct gctgcgccac cggatcaaca gattctggtt gcggggctgc gtcgtcctgc
     8461 tgcacggttt cagcatcacg catggctgct tcttctgcgg actgacgtct gccacgatat
     8521 ccggcaaccg caccgaatgg agcccccatc gcagcaccaa gagctgcacc ttcgatcgtt
     8581 gcgtcagcca cgccctccca cggtgacaca tccattccgg cggtctcacg caatgccatg
     8641 ttttcctgat agcgtgaata gccgccctgc gccgcattaa tcgccccctg ttccgtggca
     8701 tttctgacaa tgccgctttt aacggtcttc gctgtgcctc gtgtcaccag attaaacagt
     8761 tgtgcgtcac ccagttttgc cgccatggca ttcacagcca gcaattcagg atcggttgcc
     8821 agctgcgcgc gcacctcatc ggcaacacgc tcttttgcca ggtccatttt ctggcgatca
     8881 gtaagctgtg cgtgctgcgg gtcggcgtca atggacaaaa acgtctgctg aaatttcggt
     8941 gactgcgcca gctcagagta atccgcatta agaacagcat ctgctgccgc cattgcactc
     9001 tgcccctgtg cactggctgt ggaatgggtg atcaggcccg cctggaataa atccggcatt
     9061 tttttatcga cagcttctgc tgccagtgcc gtggctcttt ccggctgcac tcctgccgcg
     9121 atgtattttt tctccagccc ttcggtcagc gttcttttca gtgcgacctg tcccaccttt
     9181 ctaaccacac cgccagcaac cagatcaggt acaagcgcac caatcaggtt tacacccttc
     9241 gtcagcaaga ccgcagaatc atcatagcct tcactcattg gcgtgttcag cgcacgaaca
     9301 gcacccggcg acatctgatc tgtcagccat tcatccgagg ctttggcacc atcactgaca
     9361 gccttaccgg tgaccttcag acctttaccg acagtatcag tgaccacgtt ttttccatca
     9421 ggcagggtat cgatgacctc atcagccccc ctgctgccac cggcaaaaat atcctgaaca
     9481 gttgcgacac ccggcagccc catacggctg aactcattta aaatacgcgc ccctgttttt
     9541 accgggctct gaatcatcgc atcaccgagt ccacgggcca tttcccctgt tccccggacg
     9601 gactgggcaa cacctttacc cattgttggc aatacatcac cgaggctgaa cgatgtgctg
     9661 tgatcaatgg cattatgcgg gttgctgaaa aaatcctgcc agtaattttc tcccggctgc
     9721 ttaatgttca ggctgctgcg gttctggtta cccaattgcg cttcagggcg ctgctcatct
     9781 gaaaaagcca tacagactcc agtaaaaaaa agcccgacac gatggtcggg cgtaagtgaa
     9841 cgatattaac ggggagcgcg ttactgccgc atatttttac tttatccagc ctttgatctg
     9901 ctcagcagct gcggaagcgc tataatccag cgccttttca tcctctgcct ttttatcccg
     9961 gaggtgattc tggtaataaa tttccacctc cttttcacga ccaggttcca gcaaagcagg
    10021 catattggcg gcagcctctt ttacaaactc ctgtttaacc ggatcgcccg ccgcccacgg
    10081 ctgccacttc tgtaaaagct gttgcgtttt ccgttgctga attttctgtt gatgcggcag
    10141 tgaatcatac gctgctaaaa actcatcctt cgcagactga gcatcacgac catccttgat
    10201 cgcctgagcc actactttgc tgacttcctt ctgaagctct ttcagagaaa gtccctgctg
    10261 cttcggcatg aaatacccat aatttttcga tatttctgca aatttgctgc gatccctgac
    10321 ctgagcgata gccttatcaa cgggtatcgc cagcacggtt tgatcatcag ggtgcgcact
    10381 gccgtattct gttacaggtt tatgcgcggt ggagccatcg ctgtatgtga gatcaaggcc
    10441 aataagtacg tacccttcct gctgcgccgg tacgatactg ccaatcctgg cctcttttat
    10501 cgttttttcc ccggttgaat caggcatgcc aatacgctgt tgtagttccg gggcaaacac
    10561 gccggaaagc acatcgagat tttccggggt attcagcgaa gcaatcgccc tgtccggctt
    10621 atcatcaaag attttctgca ggttgttcac ggcctgaccc gctttcgcag catagccttc
    10681 cgttgacatt atcttaagcg gattggcgtc cgataattta ccgaacagat gagaagccgc
    10741 atcatgatca cctgcatcaa cagctttccc cagcgccacc atcaccgact gatcgcgtgc
    10801 cagcatatcg ttatactcaa gccgtcgctg gttgtatttt tccagttgca gtctctgctg
    10861 agatagccca agtgacgcat ttcgataatt ctgcgctgct ttgtgttccc tctctttcag
    10921 cgcatattca cgatcggcat tcctgacatt aagatcatat gtacgctgat cattttcttt
    10981 tcggtgctca aactgagact gcgcaaattc aaaatcgcgc tcgttatttt tctgttgctg
    11041 agcaagctgt gcttctcgca aaccaagctc cttacgacgg gtcattgcct ggtcaacagt
    11101 gctgaatccg gcaagtaacc cctgtgcaaa tccgctcatt caccactcct taaaacaaag
    11161 aaccagcaat gccgccaatt actgcaccag caacagcgcc aacaggacca ccaacggatg
    11221 cgccaatagc cgcccccgta ccaatgcccg taccgatatt ctgttttttc tgagctttct
    11281 gctgcgccgc catcatcttg tttgacgcct ccatctctgt acgtcgtctg tctgcctcgc
    11341 ttattccctg taatgcctca cgccgcgact gatttgcaat atccagtaaa ccgtatccca
    11401 tatttccccc ttacgctgcc accagttgtc cgccaacact cagtttctgt cttgccggtg
    11461 cagaagctcc cgtcagaata ttcatctggc gatcctgttc ggcttcacgg ataccatttt
    11521 tcgcgccagc aattgccagg gcattacgta accccagcgt attactgtcg ggattatccg
    11581 ggcggcttac cccgtatctc gccatctggt tatcctgcgc catctgcgct gtacggagac
    11641 cagaagtggc aaggcctccc acccgtgcaa gctgtgcatt catcagactg ttgttctccc
    11701 caaggtcagc cagccttgct actcgaggca aatatctggt gcgccagtcg tcgtattgct
    11761 ggcgggtcag cgctgcggat gtctgccagt ccccctgagg gcgggcagct ccggtataac
    11821 cgtatctcgc aagtgtttcg tatttaccgt actccataat cacagtctcc agttctgagc
    11881 ctgatgctga atggcattag cgccggtgcc aggtgtttta gcaccgcctc cgctaccgcc
    11941 agccttatgc atcgcatatg cacctgccgc acccagacca gcgccaacaa gagaggctcg
    12001 cccctgctgt ttcgtaaacg ccgcctgtgc atccgattta gcttttgcca gactgctgtc
    12061 tgccagaaag ttaaaactct gtaacgcatc cgccttctga ccagaaccga gagcagcaac
    12121 atcctgtagc ccggcaatat acttatctgc ctgcgatacc tgcccccgtg tggttgtgtc
    12181 aatctgcccg gttacctggt cgctttgatt tgcatccatt accgcattaa agcgaccact
    12241 ggacgggtca acaccggact gagcaagatt acccgccagc tccttccgtg cttcgccaaa
    12301 ctgtttctga taccccagat ttgtggtacc agcaatattg tcgtactgct gctcactgtt
    12361 caggtcatcg accttttcca tgaagttatc ttcagccggg cggaggatat ttttgtaatc
    12421 ctgccaccct ttccaggcca cttcttcctg cgctatttgc gctgctgttg gttttacttt
    12481 ggtatcacca ccgcctttac ttccacccat aatggccccc tggataacaa aaaaccctgc
    12541 cggagcaggg tcagaatgtg aattacaatg ttggtttaaa cacgatgatg aaaactgtgg
    12601 ctgtcagacc gctatcctga acaccatgaa gccatcctca tcatccggca ttcgctcaaa
    12661 gcccagacgt tttcccagcc ggataaatcc ccgccttgcc gtatgaaatt cagcccagcg
    12721 tccgccagcc agacgggtta atgtcttcac ctccggcaga taacgctcaa cgctgttact
    12781 ccccgtacac acgcccagca acaccagaac ataagggata ccatcagcac tgagcacaga
    12841 acgcagcacc agaaagccat caggtgcctc aaaacaaaac gcctgctttt taaggcaggc
    12901 gtctttaact tcattcataa attcagggtt gcgggaattt ctcaccacac gctgcatata
    12961 ccggagaatt ttttcactca cactctcacc acaaaaaaga acaatgtgac tgtgctgtgt
    13021 cttcagcgac agaaggatga cggagaacgc ttccgataac caacacggct ggcgttctcc
    13081 gcaaaaaata aattaccagc cgacagacat acccgcagca acccctgaac cgttctgggt
    13141 atcccatgaa gcattcagac gtacagcaga attttcagtg gtgcggaagt gcatacctgc
    13201 tgcgatagcc tgctcatcac ggtagttact tgcagccata ccaaaggagt acgttttacc
    13261 ctcaacaaca ggaatggcag tcatcgccat tgctcctgca atacctgcat ctgcacgttt
    13321 acggtttttc tccacctcat cacgtaactg acggaattgc tgattagtac tacgctcaag
    13381 ggtactgacc cggttatctg tgtaggtatt agcagacttc agtgtctgtg catcaccctg
    13441 aatacgggca ttctcttctg caatacgata gcgctctaac tgaccatagg ttgcggcatc
    13501 cgcagcctga acaccatcac gaagcccacc aattacacga ttccccacat taacttcatc
    13561 ggggcgagtg gtctgagagt tagcccctac agcaacactg ttcgtatagt ttgcagtggc
    13621 gttgtaacct acagcggttg cattctctcc acctgtaatt gcccctccac caacctgagt
    13681 ttctccagaa cccagtgcag acagacgagc gtggatatcc gttacatctt cagcaaaaag
    13741 tacggtatta atggtgctcc ctggtattac agacttaatt ttaatctcat aaatatattc
    13801 actaatttca ggaacatgtc ttgaatatgt tttcccatca gtacctactg tcgtgacaag
    13861 ccccttatca tctgcactaa acagaatttg atttgttttt gtattgacaa aataagcgac
    13921 cttgtttgca tagtcgtaat aagatgctat ttcactacgt ggtgctaata actctgtgtt
    13981 agtcaaacca gatttaccgg catcgtaaag agaatatttc aaatatttac cagccgtatt
    14041 ttgtatatcc acactggcag gtcctgttat tttaccattc tcaaccttta ctatactgcc
    14101 atcctggtaa cctttccctg gaaatccgag gtcattccag aacgaattta tatctgcata
    14161 ttgtttactt ttcagaacat caacagttat cccattataa ggcgaaccac taatgcctga
    14221 tgagttcgca gatgtatcta ctgcatatgc gtctgcagaa aataccgaca ggcttaatgc
    14281 cggaacgaaa gctaacaata gcttacctgt cattttcatg atgtttatcc cgtgttaatt
    14341 atgtcataaa tgcatcgaca tccgggaaca cggatgtcgt caagcataat actcacaact
    14401 acaaaata
//
LOCUS       DAZFFI010000109        14316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFFI010000109 DAZFFI010000000
VERSION     DAZFFI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.109"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            142..1275
                     /gene="ompN"
                     /locus_tag="ACHJ12_003892"
     CDS             142..1275
                     /gene="ompN"
                     /locus_tag="ACHJ12_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO4991611.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLELYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1416..1850
                     /gene="uspF"
                     /locus_tag="ACHJ12_003893"
     CDS             1416..1850
                     /gene="uspF"
                     /locus_tag="ACHJ12_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4991612.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2026..2961
                     /gene="ttcA"
                     /locus_tag="ACHJ12_003894"
     CDS             2026..2961
                     /gene="ttcA"
                     /locus_tag="ACHJ12_003894"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4991613.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3090..4463)
                     /gene="dbpA"
                     /locus_tag="ACHJ12_003895"
     CDS             complement(3090..4463)
                     /gene="dbpA"
                     /locus_tag="ACHJ12_003895"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4991614.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4493..4666)
                     /gene="ynaL"
                     /locus_tag="ACHJ12_003896"
     CDS             complement(4493..4666)
                     /gene="ynaL"
                     /locus_tag="ACHJ12_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4991615.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4941..5924)
                     /gene="zntB"
                     /locus_tag="ACHJ12_003897"
     CDS             complement(4941..5924)
                     /gene="zntB"
                     /locus_tag="ACHJ12_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4991616.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5938..5985)
                     /gene="ydaN"
                     /locus_tag="ACHJ12_003898"
     CDS             complement(5938..5985)
                     /gene="ydaN"
                     /locus_tag="ACHJ12_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4991617.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6179..7411
                     /gene="dgcM"
                     /locus_tag="ACHJ12_003899"
     CDS             6179..7411
                     /gene="dgcM"
                     /locus_tag="ACHJ12_003899"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4991618.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7432..7995)
                     /gene="smrA"
                     /locus_tag="ACHJ12_003900"
     CDS             complement(7432..7995)
                     /gene="smrA"
                     /locus_tag="ACHJ12_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4991619.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8325..9233)
                     /gene="abgR"
                     /locus_tag="ACHJ12_003901"
     CDS             complement(8325..9233)
                     /gene="abgR"
                     /locus_tag="ACHJ12_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4991620.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9409..10719
                     /gene="abgA"
                     /locus_tag="ACHJ12_003902"
     CDS             9409..10719
                     /gene="abgA"
                     /locus_tag="ACHJ12_003902"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4991621.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10719..12164
                     /gene="abgB"
                     /locus_tag="ACHJ12_003903"
                     /pseudo
     CDS             10719..12164
                     /gene="abgB"
                     /locus_tag="ACHJ12_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
     gene            12201..13727
                     /gene="abgT"
                     /locus_tag="ACHJ12_003904"
     CDS             12201..13727
                     /gene="abgT"
                     /locus_tag="ACHJ12_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO4991622.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13738..14253
                     /gene="ogt"
                     /locus_tag="ACHJ12_003905"
     CDS             13738..14253
                     /gene="ogt"
                     /locus_tag="ACHJ12_003905"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4991623.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3611 a         3586 c         3513 g         3606 t
ORIGIN      
        1 tttcagagga attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc
       61 taactgaact tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa
      121 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
      181 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagaa
      241 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac
      301 cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
      361 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca
      421 tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt cgattacgga
      481 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
      541 ggtgactctt ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact
      601 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
      661 ggtaacaacg aaggagccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
      721 catgaaaacg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
      781 gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg
      841 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
      901 ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct
      961 gttgcaaaca aaacccagaa ctttgaagtc actgcacagt accagtttga ttttggtctg
     1021 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca
     1081 gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact
     1141 tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa
     1201 gatgacagtt tctacgctgc caatggcatc tctaccgatg atattgtcgc tttaggtctg
     1261 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
     1321 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
     1381 ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta
     1441 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
     1501 agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct
     1561 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
     1621 aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc
     1681 atgttgagga aggctcgccc aaagaccgca ttctggaaat ggcgaagaag atccccgctc
     1741 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg
     1801 ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc
     1861 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
     1921 tccgtaccat acacgccact gtttttatat cagatttctt atgctgggca ttccggcatg
     1981 acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa
     2041 gaaattagta agaaagaaca atacaacctg aacaaattac aaaaacgtct gcgtcgtaac
     2101 gtgggcgaag ccattgctga cttcaatatg attgaagaag gcgatcgcat catggtttgc
     2161 ctctccgggg gtaaagacag ctataccatg ctggagattc tgcgcaattt gcagcaaagc
     2221 gcgccaatca atttttcgct ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg
     2281 gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg agtacaagat tgttgaagag
     2341 aatacttacg gtatcgtgaa agagaagatt ccagagggca aaaccacttg ctcactgtgt
     2401 tctcgccttc gtcgcggtat cctttatcgt accgcaacgg aactgggggc gacgaagatc
     2461 gcgttgggtc accatcgtga cgatatcctg caaacgttgt tcttaaatat gttctacggc
     2521 ggcaagatga aaggtatgcc tccgaaactg atgagcgatg atggcaaaca tatcgttatt
     2581 cgtccgctgg cctactgccg cgagaaagat attcagcgat ttgccgatgc aaaagcgttc
     2641 ccgattattc cgtgcaacct gtgcggttca cagcctaacc tgcaacgtca ggtgattgct
     2701 gacatgttgc gtgactggga taaacgttat ccagggcgta tcgagacgat gttcagcgcg
     2761 atgcagaatg tggtgccgtc gcatctgtgc gataccaacc tgttcgattt caaaggcatt
     2821 acccacggtt ctgaagtggt taacgggggt gatctggcgt ttgatcgcga agagatccca
     2881 ctacaaccgg cgggctggca gccagaagaa gatgaaaatc agttggatga gttacggctg
     2941 aatgtggttg aagtgaaata accaggatag cgcccgatgc gcaagcgtat cgggctactc
     3001 ttatggaggc cggataagac gcggccagcg tcgcatccgg caatcccgca taagatgttt
     3061 actcttgcac ccggcaattc aacatttcat tattttaata accgcacccg gcacgttttt
     3121 cctttaatct tcccgccctg taactgtttc catgctttat gagcaacagc ctgacgcacc
     3181 gcgacataga catgcgccgg atgcacggcg attttgccaa tatctgcgcc atcaagcccg
     3241 atgtctcctg tcagcgcacc taatacatca cccgggcgca ttttggcttt tttcccgcca
     3301 tcgatacaca acgttgccat ttctgcttcc agcggcaaaa tggaactatt agctggcggc
     3361 gtttgccagt taagttttat ctgcaacatg tcagaaatga tattggcccg ctgtgcttct
     3421 tccggagcac agaaactgat cgccagaccg ctatttcctg cacgagctgt acggccgatg
     3481 cgatgtacat gaacttcagg gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat
     3541 ttaatatcca gaccacgcgc agcaacatca gtcgcaacca gtacacgggc gctaccgtta
     3601 gcaaaacgta ccagggtctg atcgcgatcg cgttgctcca gatcgccgtg taacgacaat
     3661 gcactttgcc ctacttcatt cagcgcgtcg cagacagcct ggcaatcttt tttggtattg
     3721 caaaacacca cacaagagga tggctgatgc aaacttaata accgttgcaa cagaggaatt
     3781 ttgcctttac tggatgtctc ataaaattgt tgttcaatgg gtggcaaagc atctgttgag
     3841 tcaatttcaa tcgccaaagg atcgcgttgc actcgtccgc tgattgcggc gatggcttcc
     3901 ggccaggttg ccgaaaacag aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca
     3961 tcaatggcat cgctaaatcc catatccagc atgcggtcgg cctcatccat caccaaagta
     4021 ttcagcgcat ccagtgatac cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt
     4081 gccacgataa tatgcggcgc atgttgcaac gaatcacgct gcataccgaa cggttgacca
     4141 ccgcacaacg tcaaaatttt ggtatttggc agaaaacgcg ccagccgacg caattcacct
     4201 gccacctgat ccgccagttc acgcgtagga cacagcacta aagcctgggt ttgaaatagc
     4261 gacgcatcaa tttgctgtaa caagccgagg ccaaaagccg ccgttttgcc gctgccggtt
     4321 ttcgcctgca cgcgaacatc ttttccggca aggatcgccg gaagcgcggc ggcctgcacc
     4381 ggcgtcatgg ttaaataacc caactcatta aggttcgtga gttgggcggg aggcaaaaca
     4441 ttcagggtag aaaaagcggt cacaatctat tctcgtggtc atcgacgcaa agttagcagg
     4501 cgcgtatcct cgcagatcta cgctcacgat gcgacaattt aatcggttct tcatcaggtg
     4561 gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag
     4621 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
     4681 attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca
     4741 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
     4801 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc
     4861 ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac
     4921 agccggaaaa attgcttttg ttacaaccat ttactacggt gcaaccataa agcaacacca
     4981 ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca
     5041 gggatcccac caaggttgac gccaaataac cctgtcagaa aggtactggg taaaaagacc
     5101 attgccatca atgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg
     5161 atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aagaccgcgc
     5221 ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa
     5281 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcacg
     5341 attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga
     5401 ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg
     5461 gtcaacgcat cgcacacatc caccagccac cctccgcaat cggtcggacc cgtgccctct
     5521 tccagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
     5581 aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg
     5641 ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccgc
     5701 gtgctctcgc ccgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
     5761 cattgggcgc tatcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca
     5821 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
     5881 actgcatccg ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca
     5941 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
     6001 ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg
     6061 tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat
     6121 gacatggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat
     6181 gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga tcgtttcgcc
     6241 ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac
     6301 gtttagtcag ctacgaaccg gtccctattc cgtctcctca caaaaagaac tgccgaaata
     6361 tctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga
     6421 agaggaaaca gcattaagct gtcggcttgt tttgcgaaag ctgacagaag cagaaccggt
     6481 gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc
     6541 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
     6601 aatgttgttg attgacccct cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct
     6661 caatttctat ggttataacc atgaaacgat gtgccagaaa catacctggg aaattaatat
     6721 gctcgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa
     6781 acctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc
     6841 cgggccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
     6901 gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt
     6961 actgaatcgg cgacagtttt atcacattac ggaaccaggc caaatgcagc acctcgccat
     7021 cgcccaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
     7081 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgcaccc ttgaaagttg
     7141 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
     7201 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgtgtaa gcgtggcaaa
     7261 agtgagtatt tcgggcttac cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg
     7321 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa
     7381 tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacgact
     7441 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac
     7501 gtaacacgcc ccgctgccgc catgatgcgg tagcgcagtg caatatgcct gaacatcatc
     7561 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcatc
     7621 ccgtcctttg ccatgaataa tcagcacgtt acgcaaacca tcagccatgg cttgttgaat
     7681 gaaactgaac atcattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
     7741 ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg
     7801 ccgaaactcc agcggctgac ttagtgggat gatgtcgaga aatccggtgg tgaggaaatt
     7861 atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
     7921 gacatcggta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
     7981 gtcgtcaagg ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg
     8041 ggtcggtcca acaacgtatt acccaaaatt ccagtaataa gttccaaata ttgccgatat
     8101 tttaagcaaa atgcttatgc atgattattc attcacgata ttaataatgt aacttatatt
     8161 ttcgtgaaat ctgtcactga agaaaattgg caactaaagg ttaaaaccgt tataacacag
     8221 tcaccggcgc agaggagaca atgccggatt taagacgcgg atgcactgct gtgtgtactg
     8281 tagagtctgg cggatgtcga cagactctat ttttttatgc agttttaact ttgcagatag
     8341 ccgcattctc gccggaattg cgtgattaat gacgcggtca gtggtgtctg gcgactatca
     8401 cgccgctgaa tcaaataata ggccgctttc ggtaaaattt cgctaaccgg caacatcacc
     8461 agcccctgtc cgtgcaaggg atcgcagccc atttcttcag gcagtatgct gagaaaatcg
     8521 ctttttgcca ccagactgat acaggctgag aacgtctcgc agactacacc gacctgtggc
     8581 gtttgcgcct gatcgtcaag caattcactc aactgtttgt agtagctgcc gtgtggcgtc
     8641 ggcattgtcc agctgtaatc cagtaactgt ttgatcgaac gggcaccaat ggcggggtgt
     8701 cccgggcggc aaaagatcgc gaattgcttt tccagtaatt tctcaaaagt aaattcgtgg
     8761 tcgtacggtc cctgataata ggtattgatg gtgaaatcca attctccctg acgcaattca
     8821 ttaatcatcg acaccagttg cccttccata atgcgtactt ttacctgcgg atgctgctga
     8881 tgaaaacgag atatgacagc tggcatcaga ctgcgggaaa tactggcccc catgccgata
     8941 ttaatctgcc ctgccagttg cccttgtcgt tggcgaatat cctcttgggc tgcgcgcagt
     9001 tcttcaagaa taagactggc gtgctgataa aaactttcac cggcatcagt taacgtcacg
     9061 cctttactac ggcgaaaaaa gagttgcgcc gctaatcctt cttccagctc ctgaatagat
     9121 ttactcagtg ccggttgcga catattcaac attcggctcg ctccgcgaat gctgccctga
     9181 cgagccactt caacaaaagc acgaatttga tgaattttta cctgaaaagc catgacgcca
     9241 ccgataaccg ttatttatca gaccaaagaa actggcatct actttaatgc agatgattgt
     9301 gtcagggtaa tttatgaacg gttaaaactg tgaaaaatca gttagtgata agtaaaaact
     9361 atcgctacgt gaaccgggtc acacttttta ctgatgacgg gaaaggttat ggagtctttg
     9421 aatcaatttg ttaattcgct tgccccaaaa ttatcgcact ggcgacgtga ttttcatcac
     9481 tatgcagagt ctggctgggt ggaattccgc actgccaccc ttgttgcgga agaattgcag
     9541 cagctcggct attcactggc gctgggccgc gaagtagtta atgaaagtag ccggatggga
     9601 ctacctgatg aactcactct acaacgcgaa ttcgagcgcg ctcgtcaaca gggtgcgcta
     9661 gcacaatgga ttgcggtttt tgaaggtggt ttcactggta tcgtcgccac cctggatacc
     9721 ggtcgccccg gtccggtgat ggctttccgt gtcgatatgg acgcgctgga tcttagtgaa
     9781 gagcaggatg tcagccatcg cccctaccgc gacggttttg cgtcatgtaa cgccggaatg
     9841 atgcatgcct gtggtcatga tggacatacc gccattgggc ttgggctggc gcataccctt
     9901 aaacaattcg agtccggact acatggcgtc atcaaactga tttttcagcc tgcagaggaa
     9961 ggtacgcgtg gcgcgcgggc gatggtcgat gcaggtgtcg tagatgatgt tgattatttt
    10021 actgccgtgc acattggcac tggcgtacct gcgggcaccg tggtgtgcgg cagtgataat
    10081 tttatggcaa ccaccaaatt tgacgcgcac ttcaccggta ccgccgctca cgcaggcgca
    10141 aaaccagaag acggtcacaa tgccttgttg gcggcagcac aagccactct tgcactgcat
    10201 gcaatcgccc cgcacagcga aggagcttcc agagtaaacg tgggcgttat gcaggcagga
    10261 agcggtcgta acgttgttcc tgcctcggcg ttgctgaaag tggaaacacg cggggccagc
    10321 gacgtcatta atcaatatgt ttttgaccgt gcacaacaag cgattcaggg cgcagcaacc
    10381 atgtatggtg tcggcgttga aactcgtctg atgggtgcag ctaccgccag ttctccttcg
    10441 ccgcaatggg tcgcatggtt gcaaagtcag gcggctcagg tcgcgggggt caatcaggcc
    10501 attgaacgtg ttgaagcgcc tgcgggttcc gaagatgcca cattaatgat ggcccgcgtg
    10561 cagcaacatc aagggcaagc ctcctacgtg gtgtttggca cacagctggc ggcaggtcat
    10621 cacaacgaaa aattcgattt tgacgagcag gttctcgcta ttgccgtcga aacgctggcg
    10681 cgcaccgcgc tcaattttcc ctggacgcga ggtatctgat gcaggaaatc tatcgtttta
    10741 tcgacgatgc gattgaagcc gatcgccaac gttataccga tattgccgat caaatctggg
    10801 atcatccaga aacacgtttt gaagagttct ggtcagcgga gcatctggct tcggcgctgg
    10861 aatctgcagg cttcaccgtt acccgcaacg taggcaatat cccaaatgcc tttattgctt
    10921 cgtttggtca aggcaaaccg gttatcgccc tgctgggaga atatgacgcc ctggcaggtt
    10981 taagtcagca agcaggttgc gcgcaaccta catccgtgac gcccggtgaa aatggtcacg
    11041 gttgcggaca caatttgctg ggaaccgccg cctttgccgc tgcaatagcc gtcaagaaat
    11101 ggctggaaca atatgggcaa ggcggcacag tgcgctttta tggttgtcct ggcgaagaag
    11161 gcggctcggg taaaacgttc atggtccgcg agggggtatt tgatgatgtg gatgcggcac
    11221 tcacctagca cccggaagcc tttgccggta tgttcaatac ccgcacgctg gcaaacattc
    11281 aggcatcatg gcgctttaaa gggatcgcag cacatgccgc gaattcccct catttgggac
    11341 gaagcgccct tgatgccgta acgttgatga ccactggcac caacttcctc aacgaacata
    11401 ttattgaaaa agcgcgcgta cactatgcca tcacagatag cggcgggatc tcgcccaacg
    11461 tggtccaggc gcaggcagaa gtgctttatc ttatccgtgc ccccgaaatg accgacgtgc
    11521 agcatattta tgatcgggtc gccaaaatcg ccgaaggtgc ggcattgatg accgaaacca
    11581 cggttgaatg ccgcttcgac aaagcctgtt ccagttatct cccgaatcgc accttagaaa
    11641 atgccatgta ccatgcccta tcccattttg gtaccccgga atggaactcc gaagaactgg
    11701 cttttgcgaa acaaattcag gctacgctca cccccaacga tcggcaaaac agtctgaata
    11761 atatcgccgc aaccggtggc gaaaacggca aggtttttgc actacgtcat cgtgaaacgg
    11821 tactggcgaa tgaggtcgct ccatatgccg ccaccgataa cgtgcttgcg gcatcgactg
    11881 atgtcggcga cgtcagttgg aaactgcctg ttgcccagtg tttcagcccc tgttttgccg
    11941 tcggtacacc gctacatacg tggcaactgg ttagccaggg gcgaacatct attgctcata
    12001 aaggaatgct gctggcggcg aaaactatgg cagcaaccac agtcaatctc ttcattgatt
    12061 cagggctatt gcaagaatgc caacaagagc atcagcaagt tacggacacg caaccgtatc
    12121 actgccctat cccgaaaaac gtgacaccgt cacctttaaa ataacaacaa caacgcaaac
    12181 acaacaaccg aggaatgccc atgagtatgt catccatacc gtcgtcctcc caatccggga
    12241 agctctatgg ctgggtcgaa agaattggta acaaggttcc ccatcctttt ttgctcttta
    12301 tctatttgat aatcgtactc atggtgacga cggcaatttt gtcagccttt ggcgtcagtg
    12361 cgaaaaaccc gaccgatggt acgccggtgg tggtgaaaaa cctgctcagt gtggaaggat
    12421 tacactggtt tttacccaat gtgattaaaa actttagcgg ttttgctcca cttggagcga
    12481 tcctggcgct ggttttaggt gccggcctag cggagcgcgt cggcttactg ccagcgctaa
    12541 tggttaaaat ggcatcgcat gttaatgccc gctacgccag ttatatggtg ctgtttattg
    12601 cttttttcag ccacatttct tccgatgcgg cgttagtgat catgccaccg atgggtgcgc
    12661 tgatttttct ggcggtgggc aggcatccag ttgcaggttt actggctgcc attgcaggcg
    12721 taggttgcgg ctttacggct aatttactga ttgtcacaac cgacgtgttg ctgtcgggga
    12781 tcagcacgga agcggcggct gcgttcaatc cgcaaatgca cgtcagtgta attgataact
    12841 ggtattttat ggccagctcc gtagtcgtac tgacgattgt tggcggcctg ataaccgaca
    12901 aaatcatcga gccacgatta ggtcaatggc agggaaacag cgatgagaaa ctgcagacat
    12961 tgaccgaaag tcagcgtttt ggtttacgca tagcaggtgt cgtatcgcta ctttttattg
    13021 ctgcgatcgc gctgatggtg atcccggaaa acgggatatt gcgcgatccg attaatcaca
    13081 ccgtgatgcc atcacccttt attaaaggta tcgtgccact gatcattctt tttttctttg
    13141 ttgtctcgct ggcttatggc atcgctaccc gcacaattcg acgtcaggcg gatttaccgc
    13201 atttaatgat tgaaccgatg aaagagatgg cgggatttat cgtgatggtt tttcccctcg
    13261 cccaatttgt cgccatgttt aactggagca acatggggaa attcatcgcc gtggggctga
    13321 ccgatatact ggaaagttca gggcttagcg gcataccggc gtttgtcggt ctggcgttgc
    13381 tttcctcttt cttatgcatg tttattgcca gcggttccgc aatctggtcg attctggccc
    13441 ccattttcgt accaatgttt atgctacttg gctttcaccc ggcatttgcg caaatcctct
    13501 ttcgtattgc cgactcatcc gtattgcctt tagcgccggt atctcctttt gtcccactgt
    13561 ttcttggatt cctgcaacgc tacaaaccag acgcgaaact gggtacttac tattcgttag
    13621 tcttgcccta tccgcttatc tttttggtgg tatggctgct gatgttgctg gcgtggtatc
    13681 ttgttggtct gccgataggt ccggggattt acccacgttt gtcttaagag agaacggatg
    13741 ctgagattac ttgaagaaaa aattgccacg ccactgggtc cactgtgggt gatttgcgat
    13801 gagcaatttc gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat ggtgcagctg
    13861 ctggacatcc attatcgcaa agaaggctat gagcgcattt ctgccaccaa cccaggcggt
    13921 ttaagcgaca agcttagtga ttattttgcc ggtaatctta gcattattga tacgcttccc
    13981 actgccacag gggggacgcc atttcagcgc gaagtctgga aaactctacg cactatcccc
    14041 tgcgggcagg taatgcatta cggccaactg gctgaacaat tgggccgccc tggcgcggcg
    14101 cgtgccgttg gtgcggcaaa cggatcgaat cccatcagca tcgtcgtacc ttgccatcgg
    14161 gttattggcc gaaacggcac catgaccgga tatgcaggcg gagttcagcg aaaagagtgg
    14221 ttattgcgcc acgaaggtta tcttttgctg taaacattaa acaatttgtg ccagcttgtt
    14281 cacactttta tgtaaagtta cccttaacaa cttaag
//
LOCUS       DAZFFI010000110        14301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFFI010000110 DAZFFI010000000
VERSION     DAZFFI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.110"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            215..1273
                     /gene="citC"
                     /locus_tag="ACHJ12_003906"
     CDS             215..1273
                     /gene="citC"
                     /locus_tag="ACHJ12_003906"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4991624.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1288..1584
                     /gene="citD"
                     /locus_tag="ACHJ12_003907"
     CDS             1288..1584
                     /gene="citD"
                     /locus_tag="ACHJ12_003907"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4991625.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1581..2489
                     /gene="citE"
                     /locus_tag="ACHJ12_003908"
     CDS             1581..2489
                     /gene="citE"
                     /locus_tag="ACHJ12_003908"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4991626.1"
                     /translation="MISASLQQRKTRTRRSMLFVPSANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2500..4032
                     /gene="citF"
                     /locus_tag="ACHJ12_003909"
     CDS             2500..4032
                     /gene="citF"
                     /locus_tag="ACHJ12_003909"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4991627.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4036..4587
                     /gene="citX"
                     /locus_tag="ACHJ12_003910"
     CDS             4036..4587
                     /gene="citX"
                     /locus_tag="ACHJ12_003910"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4991628.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4562..5440
                     /gene="citG"
                     /locus_tag="ACHJ12_003911"
     CDS             4562..5440
                     /gene="citG"
                     /locus_tag="ACHJ12_003911"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4991629.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5491..6954
                     /gene="citT"
                     /locus_tag="ACHJ12_003912"
     CDS             5491..6954
                     /gene="citT"
                     /locus_tag="ACHJ12_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4991630.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7068..7874
                     /gene="rna"
                     /locus_tag="ACHJ12_003913"
     CDS             7068..7874
                     /gene="rna"
                     /locus_tag="ACHJ12_003913"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4991631.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8104..8514
                     /gene="rnk"
                     /locus_tag="ACHJ12_003914"
     CDS             8104..8514
                     /gene="rnk"
                     /locus_tag="ACHJ12_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4991632.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8652..8741
                     /gene="yldA"
                     /locus_tag="ACHJ12_003915"
     CDS             8652..8741
                     /gene="yldA"
                     /locus_tag="ACHJ12_003915"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4991633.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8745..9983)
                     /gene="ybdR"
                     /locus_tag="ACHJ12_003916"
     CDS             complement(8745..9983)
                     /gene="ybdR"
                     /locus_tag="ACHJ12_003916"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4991634.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10204..10632
                     /gene="uspG"
                     /locus_tag="ACHJ12_003917"
     CDS             10204..10632
                     /gene="uspG"
                     /locus_tag="ACHJ12_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4991635.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10753..12318)
                     /gene="ahpF"
                     /locus_tag="ACHJ12_003918"
     CDS             complement(10753..12318)
                     /gene="ahpF"
                     /locus_tag="ACHJ12_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4991636.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12489..13052)
                     /gene="ahpC"
                     /locus_tag="ACHJ12_003919"
     CDS             complement(12489..13052)
                     /gene="ahpC"
                     /locus_tag="ACHJ12_003919"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4991637.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13424..14170
                     /gene="dsbG"
                     /locus_tag="ACHJ12_003920"
     CDS             13424..14170
                     /gene="dsbG"
                     /locus_tag="ACHJ12_003920"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4991638.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3425 a         3768 c         3583 g         3525 t
ORIGIN      
        1 aaccattaaa taaataaaaa accactaact ctatgtgaaa taaatcaaaa tttcacgccg
       61 aaatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc accccccttt
      121 ctcccaagtg aaaataaaag gttatcagtt tgcaacattg aacaacattc gttgcaaatc
      181 gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata ttttcacccg
      241 cgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
      301 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
      361 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
      421 cggtgaagga ctggcgctga cattagccac tgaactgata aacctcgcct atgagcggca
      481 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
      541 tttttccacg ctgacctgcg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
      601 actgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca agattggctg
      661 cattgtgatg aacgccaatc cctttacgaa tggtcaccgt tatctgattc aacaagctgc
      721 ggcacagtgc gactggttgc atctgttttt agtcaaagaa gattcttcac gcttccccta
      781 tgaagaccgg ctggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
      841 tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
      901 gagcgtcatt aaccattgtt acaccgaaat tgatcttaag attttccgtc agtacctcgc
      961 tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
     1021 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg caccgcccat
     1081 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     1141 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     1201 gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa
     1261 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     1321 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgacctg
     1381 caaatcaata gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     1441 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     1501 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     1561 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     1621 gccgcagcat gttgtttgtg cctagtgcca atgccgcgat ggtcagcaac tccttcatct
     1681 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     1741 ccgcccgccg catggtttat cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     1801 ttgtgcgtgt caacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     1861 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     1921 ttgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc agcaccggcc
     1981 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     2041 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     2101 aacgctcccc ggaaggaact gaactgctgt tcgcacgctg ttccattttg caggccgcgc
     2161 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     2221 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     2281 cgcgtcagat tgatctgttg cacaacctct acgcaccaac ccagaaagaa gtggatcacg
     2341 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     2401 tgaacggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctctcgtg
     2461 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     2521 tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct
     2581 ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     2641 gccaacctgg aagaagcgat tcgtcgctct ggtttacagg acggcatgac ggtttccttc
     2701 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     2761 atgggcttta aaaacctgac cctggcgtcc agctccctga gtgattgcca tgcgccgttg
     2821 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctccggcct gcgcggtcca
     2881 ctggcggaag aaatctcccg cggtctgctg gcggaaccgg tgcagatcca ctctcacggc
     2941 ggtcgtgtgc atctggtaca gagcggcgaa ctgaatatcg acgtggcttt cctcggcgtc
     3001 ccgtcctgtg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     3061 ctcggctatg caatggttga tgccgacaac gcaaaacagg ttgtgatgct taccgaagaa
     3121 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tttaatcgtc
     3181 aaagttgatc gcgttggcga tgctgcaaaa atcggcgctg gtgctacccg tatgaccact
     3241 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctcaggctac
     3301 ttcaaagaag gtttctccat gcaaaccggc accggcggcg catcgctggc ggtaacccgt
     3361 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct cggtggtatt
     3421 accgcgacga tggttgatct gcacgaaaaa ggtctgatcc gcaaactgct ggatgtacag
     3481 agctttgaca gccatgctgc gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     3541 gccaaccagt acgctaactg gggttcgaaa ggcgcatcgg ttgatcgtct cgacgtggtg
     3601 gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac cggctctgac
     3661 ggcgtactgc gtggtgcttc cggtggtcac tgcgataccg cgattgcctc tgcgctttcc
     3721 atcatcgtcg cgccgctggt acgcggtcgt attccgactc tggtggataa cgtgctgacc
     3781 tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat cgcagttaac
     3841 ccggcacgtc cggaactggc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     3901 gagtggctgc gcgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     3961 gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag
     4021 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt
     4081 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgtctg gctcaagcgc
     4141 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagcgag
     4201 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cgcaaaacag
     4261 ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga gggcatgttg
     4321 agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga acatagtcat
     4381 cctctcgggc ggttatggga tatcgatgtc ctgacgcccg aaggcgaaat tctctcccgc
     4441 cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc
     4501 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac
     4561 gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga
     4621 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     4681 cctcgtggat cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg
     4741 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     4801 agaaatggca ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg
     4861 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     4921 gctgctatgt gcggcaattg gtcgtttgct tcaactcaac caaccggtaa cgccaacaac
     4981 cgtttgttct acggcggcaa gtttctgccg tggcctgacc gatcgagaac tgcgtaccaa
     5041 taattcacaa ctgacggcag gtcaacggtt gtaccaacag cttggcctta ccggcgcacg
     5101 cggtgaagcc gaagcgggtt atccactggt gatcaatcac gccttgccgc attacctcac
     5161 tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat
     5221 ggcgatcaac ggcgatacca acgttgcctc gcgcggtggc gaggggggcc tgcgctggct
     5281 acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaacccccg ccgatctcga
     5341 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     5401 cgacctgctg atccttacct ggtttttagc acagatttaa ttatttaagc acttgataaa
     5461 tttggaaata ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa
     5521 ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg
     5581 ccaccacagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc
     5641 gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt
     5701 aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag
     5761 gcgctgaaat ggggtctggc tggtttttcc agcaccacgg tatggctggt atttggcgca
     5821 tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg
     5881 gtgaaattca tgggcaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt
     5941 ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc
     6001 attaaaaacc tgccgccgct gtttaaatca ttccccaacg atccgtccgc gcgtcgtatt
     6061 ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc
     6121 accggtgcgg caccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag
     6181 attagctggt tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt
     6241 gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca
     6301 acctgggcgg gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacactg
     6361 attggccttg tattgctcag cttaggtttg tgggtttttg gcagtgaagt cattaatgct
     6421 actgcagttg gtctgctggc agtttcgcta atgctggccc tgcacgttgt gccgtggaaa
     6481 gacattaccc gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg
     6541 atggctaacg gcctgactcg ttctggcttt attgactggt tcgccggtac catgagtacg
     6601 cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt
     6661 gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg
     6721 gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg
     6781 tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac
     6841 ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt
     6901 tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt
     6961 cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta
     7021 tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct
     7081 ggcgtaacgc cgcgttgctc gcggtttctc tgcttccctt ctcttctgcc aacgccttag
     7141 cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa
     7201 ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc
     7261 aaaccgaaac gaccaacaaa gctgattttc tgaccgtaca tggtctgtgg ccaggattgc
     7321 ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta
     7381 ctcgcccaat cccgaatcta ccagaagcgc gcgccagccg aatgtgttca tcgccggaaa
     7441 ccggattatc actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac
     7501 gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg
     7561 acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa
     7621 aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg
     7681 ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg
     7741 cgtatttgac tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg
     7801 caaactcatt cttgccacaa ccacacccag gcaactgtgg caaaaccttt gtgattgata
     7861 aagtgggtta ttaatcatct gatttacaaa acacgctaat tcgtcttcca taaaccgtga
     7921 cgattaagaa ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca
     7981 cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg
     8041 tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta
     8101 aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg
     8161 ctggagcaac ccgcctatgc tggtttgcca atcgccgacg cgttaaacgc agagttggat
     8221 cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg
     8281 gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca
     8341 aaaatgaccg atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg
     8401 gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt
     8461 gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat
     8521 ctgcacaagc gataactcgc tttgcccaga ggatgcacgc gcatcctctt tacgcaaaat
     8581 cagcaatttt tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg
     8641 tgggaataac catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga
     8701 ttatcgtcat ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc
     8761 ggcatcgcat tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg
     8821 ggtaccagaa tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg
     8881 gcggcctctt caaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc
     8941 tcaattaacg gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac
     9001 gtcaaccctt tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg
     9061 cccggtacgc taacaatgcc gccacgcctg accgccgcaa tacactggcg caacgcttta
     9121 ccgctgctgc cctccagttt caggttagtc agcaccgttt ccgtggtgct gcctttcgct
     9181 tcaaaaccga cggcgtctat tactgcatcc acgccccggt gacctgccgt ttgttcaata
     9241 attgactgtg ccggatcgct atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca
     9301 gcggcgaaac gcaagcggta gggatgatga tcaacaacaa aaatctgttc cgcaccgagc
     9361 aaccgtgcac aggcgattgt caacaatccc actggaccgg caccatagac tgcaacgctt
     9421 gaaccttgtt ggatctgcgc attttttgct gcctgccatg ccgttggcag aatatcagaa
     9481 aggaaaaggg ctttatcatc tgaaagcaaa ggcggtactt taaacggccc cacattccct
     9541 ttagggacgc ggacatattc cgcctgccca ccaggaacgc cgccatacag gtgactataa
     9601 ccaaacaatg ccgcgggagc tggtatctgt tttttattga gcgcagcgcc tttacccgca
     9661 ttggtatttt cgcaggcggc atattgctgt aatcgacaga aaaaacagtc gccacaagca
     9721 atgacaaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc ctttccggtt
     9781 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
     9841 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttgccgtaat acgcagaata
     9901 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
     9961 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
    10021 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcctgg
    10081 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
    10141 atcagggata aatattataa tcatggcgaa tgatttgttc atgattaaca gggagaaagg
    10201 gttatgtata agacaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
    10261 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
    10321 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
    10381 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
    10441 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
    10501 ttggcagaag aactgggggc tgatgttgta gttattggtt ctcgcaaccc atcgatttcg
    10561 acccatctgt taggttctaa cgcctcgagc gtgatccgcc atgccaatct gccggtgctg
    10621 gttgtgcgtt aattgccaga ataaatatcc cgccctgccc ggagccatcc cggcagggct
    10681 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
    10741 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
    10801 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
    10861 cgctgcgaat acgcctttga cgttggtttc gcatttcgca tcaatgataa tctcacccat
    10921 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
    10981 gaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc gatattccag
    11041 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
    11101 gacgtttttc aggctgcgca gtttgtcctg cagaacctgg tcggctttca tttctggcgc
    11161 aaattcgagc agcgttacgt gctcaacgat acccgccagg tcaattgccg cttccacgcc
    11221 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg
    11281 cgggcagtag gtcacgcctt tggtgcgata ctgatcttcg ccaggaacgt tcatgttgcg
    11341 ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
    11401 ttcaatctga tgcagaccac cttcaactgc tgctgggatc agtttgctgg cgctctggct
    11461 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccagcttctg
    11521 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
    11581 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
    11641 agctgcaccc gccgggccgg aaccaacgat taatacgtca taagcatcac gcttgttcag
    11701 ctcttctgcc gcacgttttt ccgcgcccgt atcaattttg gcaacgattt cagtcaacgt
    11761 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
    11821 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
    11881 gttcagtacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttat ggcaagagag
    11941 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
    12001 ctgcgcttct ttcgacggat gaccaccggt ccatagcaac gccagtacca gcgaggtgaa
    12061 ctcgtggccc agcggggagc ctgcaaaacg tggcccctgg ttggaacctg ggttggtgat
    12121 caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga ctttgtctga
    12181 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
    12241 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
    12301 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
    12361 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
    12421 cccgaagaat taggtgaatt tccgaagtat ttcgcgccgg agttggtagc gcgaaagacg
    12481 aaggaaattt agattttacc aaccaggtcc agagacggag ccagagttgc ttcaccttct
    12541 ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc tgctttgatt
    12601 ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc gattgcctgg
    12661 atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc atcttcacgc
    12721 atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt
    12781 ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt atcggtagat
    12841 actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc aacgtcaccc
    12901 agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac gctccagcgg
    12961 ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc ctggttttta
    13021 aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt ttcgatgaga
    13081 tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa atcgataagt
    13141 tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc ggcgataaaa
    13201 agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag
    13261 tgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg gattacaaca
    13321 ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag tgataggtta
    13381 tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa aaaagatact
    13441 tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgctc cagtaaaagc
    13501 gattgaaaaa cagggcatta caatcatcaa aacattcgat gcccccggag gaatgaaagg
    13561 ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag atggtaagca
    13621 cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca cacttatcga
    13681 aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac aatcccactg
    13741 gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtgttcgccg atccgttctg
    13801 cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg gcaaagtgca
    13861 attaagaaca ttgttggttg gggttatcaa gccagaaagc ccggcgacag cagcggcaat
    13921 tcttgcctcc aaagatcccg caaaaacctg gcaagaatat gaatcctctg gtggcaagct
    13981 taagctaaac gtgcctgcaa acgtaagtac agaacaaatg aaagtgttaa gcgccaacga
    14041 gaaactcatg gacgatctgg gggcaaatgt cacaccagcg atctattaca tgagtaagga
    14101 aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata tcattatggg
    14161 gaataaataa aagataattc atggatggtt gaaatacatc cctgcttttc aaccatccta
    14221 aaaaagcatg aattgctcgc tctgccctaa gtggctatgc tattttagaa aaacttaatc
    14281 ctgagttaag caaaaaaata t
//
LOCUS       DAZFFI010000111        14197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFFI010000111 DAZFFI010000000
VERSION     DAZFFI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.111"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..1749
                     /gene="garD"
                     /locus_tag="ACHJ12_003921"
     CDS             178..1749
                     /gene="garD"
                     /locus_tag="ACHJ12_003921"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4991639.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1898..2233
                     /gene="prlF"
                     /locus_tag="ACHJ12_003922"
     CDS             1898..2233
                     /gene="prlF"
                     /locus_tag="ACHJ12_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4991640.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2233..2697
                     /gene="yhaV"
                     /locus_tag="ACHJ12_003923"
     CDS             2233..2697
                     /gene="yhaV"
                     /locus_tag="ACHJ12_003923"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4991641.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACHJ12_003924"
     CDS             complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACHJ12_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4991642.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACHJ12_003925"
     CDS             3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACHJ12_003925"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4991643.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5113..5586
                     /gene="agaV"
                     /locus_tag="ACHJ12_003926"
     CDS             5113..5586
                     /gene="agaV"
                     /locus_tag="ACHJ12_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4991644.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5597..6376
                     /gene="agaW"
                     /locus_tag="ACHJ12_003927"
     CDS             5597..6376
                     /gene="agaW"
                     /locus_tag="ACHJ12_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4991645.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6366..7244
                     /gene="agaE"
                     /locus_tag="ACHJ12_003928"
     CDS             6366..7244
                     /gene="agaE"
                     /locus_tag="ACHJ12_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4991646.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7262..7696
                     /gene="agaF"
                     /locus_tag="ACHJ12_003929"
     CDS             7262..7696
                     /gene="agaF"
                     /locus_tag="ACHJ12_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4991647.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7693..8826
                     /gene="nagA"
                     /locus_tag="ACHJ12_003930"
     CDS             7693..8826
                     /gene="nagA"
                     /locus_tag="ACHJ12_003930"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4991648.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9177..10331
                     /locus_tag="ACHJ12_003931"
     CDS             9177..10331
                     /locus_tag="ACHJ12_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4991649.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHJ12_003932"
     CDS             10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHJ12_003932"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4991650.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11371..11847
                     /gene="agaB"
                     /locus_tag="ACHJ12_003933"
     CDS             11371..11847
                     /gene="agaB"
                     /locus_tag="ACHJ12_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4991651.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11886..12689
                     /gene="agaC"
                     /locus_tag="ACHJ12_003934"
     CDS             11886..12689
                     /gene="agaC"
                     /locus_tag="ACHJ12_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4991652.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12679..13470
                     /gene="agaD"
                     /locus_tag="ACHJ12_003935"
     CDS             12679..13470
                     /gene="agaD"
                     /locus_tag="ACHJ12_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4991653.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13471..>14197
                     /locus_tag="ACHJ12_003936"
     CDS             13471..>14197
                     /locus_tag="ACHJ12_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO4991654.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLH"
BASE COUNT         3424 a         3607 c         3715 g         3451 t
ORIGIN      
        1 aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctgaa atgattgaca
       61 ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg aagtcattta
      121 tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata ttgcacaatg
      181 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
      241 gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat
      301 gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg
      361 gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg tgcaatccca
      421 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
      481 ctgccactgg caaccaaagt cccggccccc ttaccgccgc tggaaggata cacctttgag
      541 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
      601 gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg cgatctgcta
      661 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ctgtggcgtg
      721 gcgattaacg caccggcggc agttgtgcct attcgtacca tccacaatat ttcgctgaac
      781 cctaactttg gcggcgaagt aatggtgatt ggtctgggtt gtgaaaagtt gcagcctgag
      841 cgcctgctgg tcggcacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc
      901 agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc
      961 gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa
     1021 ctggttgtcg gcatgcagtg cggtggcagc gatgcctttt ctggcgtaac ggcaaacccg
     1081 gcggttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat gttctcagaa
     1141 gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     1201 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc
     1261 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa cgtggtagag
     1321 aaggcactcg gctccattgc taaatcgggt aaaagcgcaa ttgttgaagt gctgtcgccc
     1381 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     1441 tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt
     1501 acgccgtacg gcctgatggc ggtacccgtc attaaaatgg cgacccgcac cgagctggcg
     1561 aaccgctggt ttgatttaat ggatattaac gcgggcacta tcgccaccgg cgaagaaacc
     1621 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     1681 aaaaccttct cggatcaatg ggggctgcat aaccagctgg cagtgtttaa cccggcaccg
     1741 gtgacctgat ttcttttgga acgtccctcg caaaacatgg ccttagtgcc atgtttttat
     1801 tgtttaaagc ccccacgtcc attaataatg catttgcatt accttagttc aagcttataa
     1861 tttgagcaga aaacaggctg taaaaggaca gtgaatcatg cccgctaatg ctcgctctca
     1921 cgctgtactg accactgaat caaaggtcac gatacgcgga caaacaacta tccccgcgcc
     1981 agtgcgtgag gccttaaaac tgaagccagg ccaggacagc attcattacg aaattctgcc
     2041 tggtgggcaa gtctttatgt gccgactggg agatgaacag gaggatcata ctatgaatgc
     2101 atttttgcgt tttctggatg cagatatcca gaacaacccg caaaaaactc gtccattcaa
     2161 cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg
     2221 cgacgacgaa taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc
     2281 tgttttcagg aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat
     2341 cctgaaaatt atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt
     2401 gaggagcata tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc
     2461 tctgggaaaa ataaagactg gtcacgggta aaatttggtg ctggtcgtta tcgtctcttc
     2521 tttcgttata gtgaaaaaga gaaagtcatc attctgggat ggatgaacga tgaaaacact
     2581 ctgcgcacct acggtaaaaa aacagatgcc tataccgtat tcagcaaaat gttaaaaaga
     2641 ggacatcctc ctgccgactg ggaaagcctc acccaagaga cagaagaaaa ccattgatgg
     2701 tgttcacaat gcccgccgaa cgtattcgtg ttgaccagcg ggcacccttt ctcactcccc
     2761 gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg caggaatgcc
     2821 ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggaacg
     2881 attgaattta ctggaatcgg tgaccacgat gatccgttcc gcaacttcgc acatccgacg
     2941 gtttaaacgg gcttcatctt cattatgtgt gctgacgccg cgctccagat cgatcgcatc
     3001 tacaccaaga aacagcatat cgaagtggta attttgcagc gattgctcag cctgatcgcc
     3061 gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc
     3121 ggcttccagc aacgcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgtg
     3181 cttacgcatc agacgagcaa tctcaaaagt ggtagtcccg gaatcgagga tcacccgatg
     3241 accaggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg cggtgttcag
     3301 cgcgctttta tcttccactg atggctcgac tgacggcgtc gtgctatcgc agatcaacgc
     3361 gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat cattgcggat
     3421 cgtcacagta gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgctg
     3481 tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg ccagtcactc gcttctcacc
     3541 tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt
     3601 gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg gcgcaagcta
     3661 ctctcgtttc agtgactttc attatgtttc ttttgtgaat cagatcagaa aactattatc
     3721 tttcgtttta tttttatctc accatgacgc agtatcaact gaaacaaaac gaaagattaa
     3781 tatcgcagta atctgaactg gagaggaaag tgaaacatct gacagaaatg gtgagacagc
     3841 acaaagcggg caaaacaaat ggaatttatg ccgtttgttc cgcacatccg ctggtgctgg
     3901 aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa gcaacctcca
     3961 atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt cgcggctttg
     4021 tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gttgattctg ggtggtgacc
     4081 atctggggcc aaaccgctgg caaaacctgc cggccgctca ggcaatggcc aatgccgatg
     4141 atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt
     4201 cctgtcagga cgatccgatt cccttaactg atgacatcgt ggctgaacgc gccgcccgtc
     4261 tggcgaaagt ggcggaagaa acctgtcttg aacactttgg cgaagccgat ctggagtatg
     4321 tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc gagctggcgg
     4381 tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc
     4441 aaggtttgaa tgccatctgg ccacgcatca ttgccctggt ggttcaaccc ggcgtcgaat
     4501 tcgatcacac caacgttatt gattatcagc ccgccaaagc gagcgcctta agccagatgg
     4561 tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa acgccgcaat
     4621 cgctgcgcca gctggtgatt gaccactttg ccattctgaa agttggccca gcgctgacct
     4681 tcgccctgcg tgaagctctg ttctctctgg cggcgattga agaagaactg gtgccagcga
     4741 aagcctgttc tggtctgcgt caggtgctgg aagacgtgat gctcgaccgc ccggaatact
     4801 ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt tatagctact
     4861 cggatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc gctcatctgg
     4921 ttcgtaatct ggcggattca ccaattccgc taccgctcat cagccagtat ctgccgctgc
     4981 aatacgtgaa agttcgctcc ggcgagctgc aaccgacgcc acgggaactc attatcaacc
     5041 atattcagga catcctggcg cagtactaca cagcctgtga aggccaataa gcaaaacaaa
     5101 gaggaacacg ctatgccaaa tattgtttta agccggattg atgaacgctt gattcacggt
     5161 caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc tggtgctggt agccaacgat
     5221 gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc agaagggatc
     5281 gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga
     5341 cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt gaaaggtggc
     5401 gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa acaacaaatc
     5461 gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct gaaagccgct
     5521 ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct ctttaaatta
     5581 ctttgaggga ttcatcatgg aaatcagcct gttgcaggca tttgcgttgg gcattatcgc
     5641 ctttatcgct ggcctagata tgtttaacgg cctaacacat atgcaccgcc cggtggtcct
     5701 cggcccgttg gtcgggctgg tacttggcga tctgcatacc ggaattttaa ccggcggtac
     5761 gctggaactg gtgtggatgg ggctggcccc gctggcgggc gcacagccgc ctaacgtgat
     5821 tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc
     5881 ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct tcctgttctc
     5941 ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg atactcgcgg
     6001 cattgaacgc gtgaactatc tggcactgct ggcactcggc actttctatt ttctctgcgc
     6061 tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg tcctgccgca
     6121 acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt
     6181 gctgctgaaa atcatgatga aaaacgtcta catcccctac ttcatcctgg gctttgttgc
     6241 cgcagcctgg ctcaagttac cggtgctggc tattgctgcc gctgcgctgg cgatggcgct
     6301 gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga aagaggaatt
     6361 tgaagatggc atctaatcaa accaccctgc cgaacgtcag tgaaaacgaa gaaacattgc
     6421 tgactggcgt caatgaaaac gtgtatgaag atcagagcat tggcgcggag ctgacgaaaa
     6481 aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca agcgtctttt aactacgaac
     6541 gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc tctgaaaaag atccacacta
     6601 ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat acccatccgt
     6661 ttctggtgac atttgttatc ggcattatcc ttgcgatgga gcgttctaag caggacgtta
     6721 acagtattca gagcaccaaa attgccgtcg gtgcgccgct cggcgggatt ggcgatgcaa
     6781 tgttctggct aacgctactg ccgatttgtg gcgggattgg agccagtctg gcattgcaag
     6841 gctccattct tggcgcagtc gtctttattc tgctgttcaa cgtggtacac ctggggctgc
     6901 gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattcca ctcattaaag
     6961 cgaataccaa aaaagtcggc catgctgcgt ctatcgttgg gatgacggta atcggcgcgc
     7021 tggtggcaac ctatgttcgt ttaagcacca cgctggaaat cacagcgggc gacgcagtgg
     7081 ttaagttaca gactgatgtt attgacaaac tgatgcctgc cttcttaccg ctggtctaca
     7141 ccttgaccat gttctggctg gtacgccgcg gctggagtcc gctgcgtctg attgcagtta
     7201 ccgtggttct cggcatcgtc ggtaaattct gccatttcct ttaaatacaa tgaggtatta
     7261 gatgttaagt attattttga cagggcatgg cgggtttgcc agcggcatgg aaaaagcaat
     7321 gaagcaaatt ctcggtgagc aatcgcagtt tatcgccatc gattttccgg aaacctcaag
     7381 caccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt gtgaagacgg
     7441 cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt caacgctggc
     7501 gatgcaaaaa ccgggctgtg aagtgatcac cggcaccaat ttgcaactgc tactggagat
     7561 ggtgctggag cgcgaagggt taagcggtga agaattccgt gtacaggcgc tggagtgtgg
     7621 acaccgaggg ctgaccagtc tggtggacga gttaggccgc tgccatgaag aatgtccggt
     7681 cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga agagggatgg
     7741 ctcgatgacc atcagttgcg tattgctgac ggtgtcatcg cagcaatcga accgattcca
     7801 gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg cttacatcga cacccatgta
     7861 cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga caagctggca
     7921 atgcacaagg cacgcgaagg tgtcggcagt tggttgccga ctaccgtaac cgcgccgctt
     7981 aacagcattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt
     8041 gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg
     8101 catccgccgg aattgtttcg cgagcttgaa attgccgagc tggatcaatt gattgccgtt
     8161 tctcagcaca ccttacgcgt ggtagcgctg gcaccggaaa aagagggggc attgcaggcc
     8221 atccgccatc ttaaacagca aaatgtacga gtgatgctgg ggcatagcgc ggcgacctgg
     8281 caacaaactc gcgccgcgtt tgatgctggt gccgacggcc tggtgcattg ctataacggg
     8341 atgacaggtt tacatcaccg cgaaccggga atggttggcg cgggattaac ggacaagcgc
     8401 gcctggctgg aactgatagc cgatggtcat catgtgcatc cggcggcgat gtcgctgtgt
     8461 tgttgctgtg caaaagagag aatcgtgctg atcaccgacg cgatgcaggc agccgggatg
     8521 ccggatggtc gctatacgtt atgtggcgaa gaagtgcaga tgcacggtgg cgttgtccgt
     8581 accgcgtccg gtgggctggc gggcagtacg ctgtctgttg atgcggcagt gcgcaacatg
     8641 gtcgagttga cgggcgtaac gcctgcggaa gccattcata tggcatcgct gcatccggcg
     8701 cgaatgctgg gtgttgatgg tgttctggga tcgcttaaac cgggcaaacg cgccagcatc
     8761 gttgcgctgg atagcgggct gcatgtgcaa caaatctgga ttcagagtca attagcttcg
     8821 ttttgatatt ttgctccttt attgggcctt cacttccccc gtaaggcctt tctttttctt
     8881 tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaac gcgtcttatg cgacctacgg
     8941 tacgccgcat ccagcaatcg gtgaacatag cgacgtcatt acgcaattct ttaatcttcc
     9001 cttgtctttt ctttcaatga tcacagattt cgttttattt cctttttctc cattgaactt
     9061 tcggtttctt ttctatagat tttaatcaac gaaagacatc accaagtgaa atgaaacgaa
     9121 aggcaagtga aagcgacaac gcccgacgtc aagttcatca gactaaggat tgagttatgc
     9181 cagaaaatta cacccctgct gccgccgcaa ccggtacatg gactgaagaa gagatccgcc
     9241 atcagcctcg cgcatggatc cgttcactca ccaacatcga cgcgctacgt tctgcgctca
     9301 ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg accggagccg
     9361 gaacctcggc atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa
     9421 acttcagcgc cgtaccgacc accgatctgg tcaccaatcc gatggactac ctgaacccag
     9481 ctcatccgct gctgttgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgctg
     9541 ccgtggaact ggcaaatcaa tttgtaccag aatgctatca cctgccgatc acctgcaacg
     9601 aagcgggcgc tctttaccaa aacgcgatca acagcgataa cgcgtttgcc ctgctgatgc
     9661 ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag cattaccacc atgatggcca
     9721 gctgcctcgc ggttttcgca cctgagacga tcaacagcca aaccttccgc gacgtggcgg
     9781 atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg
     9841 caccgtggaa acggatcgtt tatctcggca gcggtggctt acagggcgca gcacgcgagt
     9901 cggcgctgaa agtgctggaa ctgactgcgg gtaaactggc ggccttctat gattccccga
     9961 ccggattccg tcatggcccg aaatcactgg tcgataacga aacgctggtg gtggtatttg
    10021 tctcaagcca cccttacacc cgtcagtatg atcttgatct gctggctgaa ctccgccgtg
    10081 acaaccaggc aatgcgcgta atcgccatcg ccgcggaaag caccgacatc gtcgctgccg
    10141 gtccacatat tatcctgccg ccgtcacgtc actttatcga cgttgagcag gcattttgct
    10201 tcctgatgta cgcccagacg tttgcactga tgcagtcgct gcacatgggc aatacgccgg
    10261 ataccccatc agccagcggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt
    10321 ggcaggcata agaggatcgc attatgagca ttatctccac taaatatctg ttacaggacg
    10381 cccaggccaa tggctacgcg gtgcctgctt ttaacattca taacgccgag acgatccagg
    10441 cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga acgccgggga
    10501 cctttaaaca tatcgcgctg gaagagatct acgccctgtg cagcgcctat tccacaacct
    10561 acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat attcgccgta
    10621 aagtccacgc aggtgtgcgc agcgcaatga tcgacggcag ccacttcccg tttgccgaga
    10681 acgtgaagct ggtgaaatcg gttgttgact tctgccactc acaggattgc agcgtggaag
    10741 cagaactggg ccgcctgggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat
    10801 tcctgaccga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc gacagcctag
    10861 cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc
    10921 ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctacatggt gccagcgatg
    10981 ttccggatga atttgtccgt cgcactattg aacttggcgt cacaaaagtg aacgttgcca
    11041 cagaattaaa aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg
    11101 gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa
    11161 ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg
    11221 atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggaa aaggagatga
    11281 ctaaacctta ataaatacat cagtacaata tcgcaacaat aatatattaa aaaaaattat
    11341 attattcaac tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt
    11401 attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgcg
    11461 aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca gaaattaatg
    11521 ggtattaccg cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt
    11581 aacgtcatcg gcaaagctgc accgcatcag aagatcttcc tgatttgccg tacgccacaa
    11641 acggtacgta aattggtaga aggtggcatt gacctgaaag atatcaacgt cggcaatatg
    11701 catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc
    11761 gcggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc
    11821 gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa
    11881 taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta gttttcgttc
    11941 tggggattga tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc
    12001 taactggcgc tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt
    12061 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg
    12121 gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc
    12181 ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct
    12241 cattatttat gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc
    12301 ggcttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct
    12361 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga
    12421 cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc
    12481 gcgtaatgtt caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct
    12541 acatccaggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg
    12601 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
    12661 aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc
    12721 tttcgttcgt cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt
    12781 acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc
    12841 gcggcgatga aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg
    12901 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc
    12961 aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg
    13021 ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg
    13081 attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
    13141 ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct
    13201 cgctcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat
    13261 attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat
    13321 ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac
    13381 ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct
    13441 attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc
    13501 tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg
    13561 ttaagtgaac gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg
    13621 atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc
    13681 caccagcagc aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat
    13741 ctgccattaa cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg
    13801 ctggggctgc gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag
    13861 tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctagga
    13921 ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc
    13981 catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc
    14041 cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg
    14101 ttattggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc
    14161 tccaccgcta tcccggcttc gtttttatgg ctgcaca
//
LOCUS       DAZFFI010000112        14086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFFI010000112 DAZFFI010000000
VERSION     DAZFFI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.112"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..1447)
                     /gene="plaP"
                     /locus_tag="ACHJ12_003937"
     CDS             complement(89..1447)
                     /gene="plaP"
                     /locus_tag="ACHJ12_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4991655.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1437..1499)
                     /gene="yoeI"
                     /locus_tag="ACHJ12_003938"
     CDS             complement(1437..1499)
                     /gene="yoeI"
                     /locus_tag="ACHJ12_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4991656.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1714..2632)
                     /gene="yeeY"
                     /locus_tag="ACHJ12_003939"
                     /pseudo
     CDS             complement(1714..2632)
                     /gene="yeeY"
                     /locus_tag="ACHJ12_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(2678..3502)
                     /gene="yeeZ"
                     /locus_tag="ACHJ12_003940"
     CDS             complement(2678..3502)
                     /gene="yeeZ"
                     /locus_tag="ACHJ12_003940"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4991657.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3585..3839)
                     /gene="yoeB"
                     /locus_tag="ACHJ12_003941"
     CDS             complement(3585..3839)
                     /gene="yoeB"
                     /locus_tag="ACHJ12_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO4991658.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3836..4087)
                     /gene="yefM"
                     /locus_tag="ACHJ12_003942"
     CDS             complement(3836..4087)
                     /gene="yefM"
                     /locus_tag="ACHJ12_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO4991659.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4370..4420
                     /gene="hisL"
                     /locus_tag="ACHJ12_003943"
     CDS             4370..4420
                     /gene="hisL"
                     /locus_tag="ACHJ12_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4991660.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4396..4519
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4566..5465
                     /gene="hisG"
                     /locus_tag="ACHJ12_003944"
     CDS             4566..5465
                     /gene="hisG"
                     /locus_tag="ACHJ12_003944"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4991661.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5471..6775
                     /gene="hisD"
                     /locus_tag="ACHJ12_003945"
     CDS             5471..6775
                     /gene="hisD"
                     /locus_tag="ACHJ12_003945"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4991662.1"
                     /translation="MSFNTIIDWSSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6772..7842
                     /gene="hisC"
                     /locus_tag="ACHJ12_003946"
     CDS             6772..7842
                     /gene="hisC"
                     /locus_tag="ACHJ12_003946"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4991663.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAILVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7842..8909
                     /gene="hisB"
                     /locus_tag="ACHJ12_003947"
     CDS             7842..8909
                     /gene="hisB"
                     /locus_tag="ACHJ12_003947"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4991664.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8909..9499
                     /gene="hisH"
                     /locus_tag="ACHJ12_003948"
     CDS             8909..9499
                     /gene="hisH"
                     /locus_tag="ACHJ12_003948"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4991665.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVHKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9499..10236
                     /gene="hisA"
                     /locus_tag="ACHJ12_003949"
     CDS             9499..10236
                     /gene="hisA"
                     /locus_tag="ACHJ12_003949"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4991666.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10218..10994
                     /gene="hisF"
                     /locus_tag="ACHJ12_003950"
     CDS             10218..10994
                     /gene="hisF"
                     /locus_tag="ACHJ12_003950"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4991667.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDENRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHIPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10988..11599
                     /gene="hisIE"
                     /locus_tag="ACHJ12_003951"
     CDS             10988..11599
                     /gene="hisIE"
                     /locus_tag="ACHJ12_003951"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4991668.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPQALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11696..12673)
                     /gene="wzzB"
                     /locus_tag="ACHJ12_003952"
     CDS             complement(11696..12673)
                     /gene="wzzB"
                     /locus_tag="ACHJ12_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4991669.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12820..13986)
                     /gene="ugd"
                     /locus_tag="ACHJ12_003953"
     CDS             complement(12820..13986)
                     /gene="ugd"
                     /locus_tag="ACHJ12_003953"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4991670.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAECFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3558 a         3632 c         3574 g         3322 t
ORIGIN      
        1 tggttattaa tgaggatgtc gataaaaaag aataaaaaaa ccggagccaa atattcattc
       61 gactccggtt tattattaca aaagatgatt acgctacgtc ttcgtactgc ggaaccgggt
      121 tacggaaact cttggtgacg caagccaggt aaatcagacc gatagccgcc cagatcagac
      181 ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac
      241 acatcggcag gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga
      301 tccagaactg cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt
      361 taatcagcgc tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa
      421 ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt
      481 tcgggaatac gccgtcacga cccattacgt acatcagacg cgctacgcct gcatgcgccg
      541 ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt
      601 tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga
      661 tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca
      721 gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca
      781 ggttgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca
      841 tcgggataac atgcgcatcg ccagaccaga acggacgagt gctcgccaga gtaccagcgc
      901 cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct
      961 gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg
     1021 cggtcatgaa ggccaccagc gccaccacaa acatccacga tgggatggaa ggcactagag
     1081 cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca
     1141 gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtgt
     1201 atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca
     1261 ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa
     1321 cgataccaaa agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa
     1381 ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat
     1441 gcgacatggt cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt
     1501 tttttggatt cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa
     1561 gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac
     1621 gtcggttttt agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca
     1681 agagaatgag aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga
     1741 ttttgcataa acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct
     1801 ggcaagccct gcggtctgtt cattccagcc aatccagacg ggttcaaaga gagtttgggc
     1861 gaccactttc ttctcgatta acgcgccact atcgagaaac cgttgcgcca gataacgggg
     1921 taaatagccg cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac
     1981 ggtaatggct tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt
     2041 atcccccacc acaatcgccc gatatcgctt aataatacga cggtttaacg gctcttcttc
     2101 cagagctaac ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga
     2161 aaaaccaaac tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg
     2221 cccctgagtc agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt
     2281 cacgctatga tgttgataaa aagcttcaat cagtggcgca aggaaacgtg tcgtccacgc
     2341 caatcaccag ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc
     2401 gcacggtatg cagaacttcc cgccctttct ctaataacat ttttccggta cgggtgaatt
     2461 tagcgcggtg accgctacga tcaagcaatt gaatattaag gtcgctttcc agcttgtgaa
     2521 cggtgtaact cagagcggac ggcgtcttat agagtttggc cgacgccgcc gcaaaactgc
     2581 cttctttttc taaggcatcg aggatcatca acacatccag cagtggtttc atacacgccc
     2641 ccttgcagtg tatgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg
     2701 gatcgggata ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct
     2761 tacctttgcc gctgtccaga ctatttctga actgcggagg ctccagcccc agtaaacggg
     2821 ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt
     2881 gtccgccttt aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga
     2941 ctaaattaac accatgttca ccatcaggcg cggtttttcc ggcaaagaag cgtccgggat
     3001 gacgtcccgg tccgaccagc cccgcaagac gcagaatatc gaccgaagta ccgggtaaat
     3061 tgtgcagcca gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg
     3121 gggtagtttc tttcaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa
     3181 taatgcgggg aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat
     3241 agaactcatc gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcgacat
     3301 ccatcagggc atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat
     3361 agctatcaat gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg
     3421 tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg catgcctaac caccctaacc
     3481 cgacaattgc gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac
     3541 gctagcgtat caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga
     3601 catgctgcaa tgagcagtga atcgtcggta accgcgtata ccagacggtg atcctctgta
     3661 atgcgtcggg accagaaacc tgacaaatta tgtttcaggg gttctggctt ccccttacct
     3721 tcaaatggcg ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta
     3781 tctgtttcct gccagtacag ataatcgtcc catgattcct cagaccagat tagtttcact
     3841 caataatgtc cttttctgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
     3901 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
     3961 cgagtgacat cagaacacaa gcctctccat tctgacgagt aataaggatc ggggcatgat
     4021 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
     4081 tacgcatgtc aatctcctct tttgtacagt tcattgtaca atgatgggcg ttaattaact
     4141 atttattaat tagtttgtag atcaaggtat tgtcagtgag acgaaaatcc aggcttcgct
     4201 atttttggtg ccgtcagctt agaggacagt cctcttagcc ccctcctttc cccgctcatt
     4261 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
     4321 tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta tgacacgcgt
     4381 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
     4441 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
     4501 cttccggggg cttttttatt gcgcggttgc taacggttca gacaggttta aagaggaata
     4561 acaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
     4621 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
     4681 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtacgtgac gacgacattc
     4741 ccggtctggt aatggacggt gtggtagacc ttgggattat cggcgaaaac gtgctggaag
     4801 aagaactgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     4861 tggatttcgg cggctgccga ctttcactgg caacgccggt tgatgaagcc tgggacggcc
     4921 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     4981 tcgaccagaa aggcatctct tttaaatcct gcttactgaa cggttctgtc gaagtcgccc
     5041 cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     5101 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     5161 atggcgaaat ggaagaatcc aaacagcaac tgatcgacaa actgctgacc cgtattcagg
     5221 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgtctgg
     5281 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     5341 accaacaacg cgtagcgatg cacatggtca gtagcgaaac cctgttctgg gaaacgatgg
     5401 aaaaactgaa agcgctgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     5461 agtgatcgcc atgagcttta acacaatcat tgactggagt agctgtactg cggagcaaca
     5521 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     5581 cgatattctc gataacgtga aaacgcgtgg cgatgaagcc ctgcgtgaat acagcgcgaa
     5641 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gaggaaatcg ccgccgccag
     5701 cgaacgcctg agcgacgagt taaaacaggc gatggcggtg gcagtaaaga atattgaaac
     5761 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtacgttg
     5821 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attcctggcg gctccgcccc
     5881 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcaggtt gcaataaagt
     5941 ggtgctgtgc tcaccgccgc cgattgccga tgagatcctt tacgcggcgc agctgtgcgg
     6001 cgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     6061 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     6121 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggtccgtc
     6181 ggaagtgctg gtgattgctg acagcggcgc tacgccggat ttcgtggctt ctgatttgct
     6241 ttctcaggct gaacacggcc cggactcaca ggtgatttta ctgacgcccg ctgctgatat
     6301 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     6361 cgcccgccag gcactgagcg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     6421 agagatttct aatcagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     6481 gctggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     6541 ggcaggcgac tatgcctccg gtaccaacca cgtactaccg acttacggtt ataccgcgac
     6601 gtgttccagc ctcggcctgg ctgatttcca gaagcgtatg accgtgcagg agctgtcgaa
     6661 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     6721 cgcccacaaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag catgaacacc
     6781 gtgactatta ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     6841 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaataccc cacagccgtg
     6901 gagtttcagc ttactcagca aacgctcaac cgctacccgg aatgtcagcc gaaagcagtg
     6961 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc
     7021 gcggacgaag gtattgaact actgattcgc gctttttgcg aaccgggtaa agacgccatc
     7081 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggcgtcgag
     7141 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     7201 ctggacggcg taaaagtggt ctatgtttgc agccccaaca acccaaccgg gcaactgatc
     7261 aatccgcagg attttcgcac cctgctggag ttaacgcgcg gtaaggcgat tttggttgcc
     7321 gatgaagcct atatcgagtt ttgcccacag gcatcgctgg ctggctggct ggcggaatat
     7381 ccgcacctgg ctattttacg cacactgtcg aaagcttttg ctctggcggg gcttcgttgc
     7441 gggtttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat cgccccctac
     7501 ccgctctcga cgccggttgc cgacattgcg gcccaggcgt taagcccgca gggaatcgtc
     7561 gctatgcgcg aacgagtgac gcaaattatt gcagaacgcg aatacctgat tgccgcactg
     7621 aaagagatcc cctgcgtaga gcaggttttc gactccgaaa ccaactacat tctggcgcgc
     7681 tttaaagcct ccagcgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     7741 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     7801 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     7861 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     7921 ttgacaaact cgcctttgaa ccgggcgtga tcccggagct gctaaagctg caaaaagcgg
     7981 gctacaagct ggtgatgatc actaatcagg atggtcttgg aacacaaagt ttcccgcagg
     8041 cggatttcga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtacagt
     8101 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     8161 aagtaaaact ggtggagcgt tatctggctg agcaagcgat ggatcgcgcc aacagttatg
     8221 tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     8281 gctacgaccg cgaaaccctg aactggccga tgattggcga gcaactcact aaacgagacc
     8341 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgttcag gtgtggctgg
     8401 atcgtgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     8461 agatcgccac ccacggcggt ttccgtatgg aaatcaacgt caaaggcgac ctctatatcg
     8521 acgatcacca caccgtcgaa gataccggcc tggcgctggg tgaagcgtta aaaattgccc
     8581 ttggcgacaa acgcggtatt tgccgctttg gttttgtgct accgatggac gaatgccttg
     8641 cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     8701 accagcgcgt gggcgatctc agcactgaaa tgatcgagca cttcttccgt tcgctctctt
     8761 acaccatggg cgtgacgctg cacctgaaaa ccaaaggtaa aaacgatcac caccgtgtag
     8821 agagcctgtt caaagccttt ggtcgcaccc tacgccaggc catccgcgtg gaaggcgata
     8881 ctctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     8941 caatttgaac tcggtgaaat ctgccattgc gcgtcacggt tatgaaccga aagtcagccg
     9001 tgacccggac gtcgtgttgc tggccgataa actgttttta cccggcgttg gcactgcgca
     9061 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     9121 accggtgctg ggcatctgct tagggatgca attgctgggg cggcgcagcg aagagagcaa
     9181 cggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     9241 gccactgccg catatgggct ggaaccgcgt ttacccgcag gcaggcaacc gcctgtttca
     9301 ggggattgaa gacggcgcgt acttttactt tgttcacagt tacgcaatgc cggtcaatcc
     9361 gtggaccatt gcccaatgta attacggcga accgttcacc gcggcggtac acaaagataa
     9421 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     9481 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     9541 cgtctccatc agggcgatta cggcaaacag cgcgattacg gtaacgaccc gctgccgcgc
     9601 ttacaggatt acgccgcgca gggtgccgaa gtgttgcatc tggtggatct gaccggggca
     9661 aaagatccgg ctaaacgtca aatcccgctg attaaaaccc tggtggcggg cgttaacgtt
     9721 ccggtgcagg ttggcggcgg cgtgcgtagc gaagaagacg tggcggcgtt actggaagcg
     9781 ggcgttgcgc gcgtggtggt cggctccacc gcggtgaaat caccagaaat ggtgaaaggc
     9841 tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg tattgacgag
     9901 caaggcaaca agcaggtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa
     9961 caactggtgg aaacctattt gcccgtcggc ctgaaacatg tgctgtgtac cgatatctcg
    10021 cgcgacggca cgctggcagg ctctaacgtc tctttatatg aagaagtgtg cgccagatat
    10081 ccgcaggtgg catttcagtc ctccggcggt attggcgaca ttgatgatgt cgctgccctg
    10141 cgtggtactg gtgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
    10201 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc atgtctcgac gttcgtgatg
    10261 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccgc
    10321 tggcaaaacg ctacgccgaa gaaggtgcag acgaactggt gttctacgat atcaccgctt
    10381 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
    10441 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
    10501 ttggcgcgga taaaatttcc atcaactcgc ctgcgctggc ggatccgacg ttaattactc
    10561 gcctggcgga tcgttttggc gtgcagtgta ttgtggtcgg tattgatacc tggtacgacg
    10621 gcgaaaccgg taaatatcat gtgaatcaat ataccggcga cgaaaaccgc actcgcgtca
    10681 ctcagtggga aacgctcgac tgggtacagg aagtgcaaaa acgcggtgcc ggagaaatcg
    10741 tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgacctcgag cagctgaaaa
    10801 aagtacgtga agtttgccac atcccgctga ttgcctccgg tggcgcgggg accatggaac
    10861 acttcctcga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
    10921 acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag ggcgtggaga
    10981 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggact
    11041 gatgccggtg attgtgcaac acgcggtatc cggtgaagtg ttaatgctgg gctatatgaa
    11101 cccgcaagcc ctggacaaaa ccatcgaaag cggcaaagtc accttcttct cgcgcactaa
    11161 acagcgactg tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag taaatattac
    11221 gccggactgc gacaacgaca cgttactgtt gctggcgaat cccatcggcc cgacctgcca
    11281 caaaggcacc agcagctgct tcggcgacac cgctcaccag tggctgttcc tatatcaact
    11341 ggaacaactg ctcgccgaac gcaaatctgc cgacccggaa acctcttaca cagccaaact
    11401 gtatgccagt ggcaccaaac gcattgcgca gaaagtgggt gaagaagggg tggaaaccgc
    11461 actggcagcg acagtacatg accgctttga actgaccaac gaggcatctg atttgatgta
    11521 tcacctgctg gtgttgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
    11581 gcgtaaacgg catcagtgag ttgcggggta agcggatgcg atgttgttgt cgcatccggc
    11641 aaaaaaaacg ggcaaggtgt caccaccctg cccgttttct ttaaagctgc aaagattact
    11701 tcgcgttgta attacgcaga gcattacgcc ccaacacaat ccccgcgcca accatgccgc
    11761 ccagcagcac cgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta
    11821 acgtcggttt catcacataa cggtaagcat gaatatcaag tttatcaacg tcaagattgt
    11881 cgatatccag caggttttga cgagtctgat agtagtttga tgagaacacc aacggacgag
    11941 tcgcctcgtg cttaatcatc gactccagcg cttcgctccc caaaaggaac atagtatcct
    12001 gggttacgtc ctgtgtctgc tgaatctgcg gctttgtcac ctgcgcctga ttcgcatact
    12061 gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgca accacttcct
    12121 gggtcttcag agaatcctgc aagtttttca tttgcagcgc gatgttgtct ttcaggtctt
    12181 gttccagttc ttcatttacc tggtcgtcaa cttgctggat atactgcgcc agctgtttct
    12241 gcgcaccttc aggtgtttca cccacataag aaaccgctaa aggtaatgac tgatttttaa
    12301 cggtaggctc aatggtcagt ttttcaggtt cttcctgatt atccagcgtt tctgctaatg
    12361 cagaaaatgt cgtgctgaaa cgaccaatta acatcgactg gatctcagac actttcggcg
    12421 ctgccgaacc ataaatcaca tttatagcat tgttataacc agcgatttgc cccacatcag
    12481 gttgagtaac gatagctgta gaagtccact tttctttggc aacggccaga taccctatag
    12541 ccagggcaat agcgacaatg acagagatga taattgtcat cttgccacgc cacaactgca
    12601 ccagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat
    12661 tttctactct catcattacc ctaacggacg aaataatttt aagtcgccat agtttagcga
    12721 taatcgccat gaatgctata ggataaatga taaaaataat gaattattac aaagggagca
    12781 taagagaatc agggcgaaaa tcgccctgat aacaagatgt tagtcactgc caaagagatc
    12841 gcgggtgtag actttgtctg ccacatcctt aagctcttct gccatacggt tggagataat
    12901 gacgtcggct tgttgtttga aggtggcgag atcacgttcc aggcgagagt tgaagaatga
    12961 gtcttctttc atcactggct cgtatatgat cacttcaacg cctttcgcct tgatacgctt
    13021 cataatcccc tgaatggaag acgcgcggaa gttatctgaa ccgctcttca taatcagacg
    13081 ataaataccc accacttgcg gtttgcgtga caaaatggca tcggcaataa agtctttacg
    13141 tgtgcggtta gcatcgacaa ttgccgaaat caggttattc ggcacagact ggtagttcgc
    13201 cagtaactgc ttggtatctt tcggcagaca ataaccacca tatccaaacg acggattgtt
    13261 gtaatggttg ccaatacgcg gatcgagaca aacgccttcg ataatctggc gagtattcag
    13321 ccctaaactt tctgcatagc tatccagttc attaaagtat gctacgcgca tcgccagata
    13381 ggtattagca aacagtttaa tcgcttctgc ttcagtggag tcggtaaaca gtgtagggat
    13441 attttgctta atcgcgcctt cctgtaataa cgccgcaaaa cattctgcgc gttctgaacg
    13501 ctcaccgatg acaatacgtg acggatggag attatcgtaa agggctttac cttcacggag
    13561 aaattccgga gagaatataa tattttcagt gcgatatttc ttatgcatcg ctgcggtaaa
    13621 accaacggga accgttgatt tgatcaccat aaccgcataa ggatttatct caactacgtc
    13681 tttaattact gattctacac tggatgtatt gaaataatta gttttaggat catagtcggt
    13741 tggagtggcg atgatgacat aatcagcatc ccggtaggct tcatttttat ctaatgtggc
    13801 attaaagtgt attttatctg attgcaaaaa ctgctgaatt tccttatcaa caataggaga
    13861 tatccgatca ttcagcatag caacgcgtga cggtaaaata tctaatgcca caacctcatg
    13921 attttgtgcg attagaagcc cgtttgacaa gcctacatag ccagtaccgg aaatggtgat
    13981 tttcatttca tgctctcaga attaacttaa ctgtgaatca tgatgttttt agcatcctga
    14041 taagagctaa atgttttaac gctacgttat tagatggtca acgcta
//
LOCUS       DAZFFI010000113        13911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFFI010000113 DAZFFI010000000
VERSION     DAZFFI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.113"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            223..312
                     /locus_tag="ACHJ12_003954"
                     /pseudo
     CDS             223..312
                     /locus_tag="ACHJ12_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            786..887
                     /gene="ykgR"
                     /locus_tag="ACHJ12_003955"
     CDS             786..887
                     /gene="ykgR"
                     /locus_tag="ACHJ12_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO4991671.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1250..1513
                     /gene="ykgM"
                     /locus_tag="ACHJ12_003956"
     CDS             1250..1513
                     /gene="ykgM"
                     /locus_tag="ACHJ12_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4991672.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            1513..1653
                     /gene="ykgO"
                     /locus_tag="ACHJ12_003957"
     CDS             1513..1653
                     /gene="ykgO"
                     /locus_tag="ACHJ12_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO4991673.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1688..1915)
                     /gene="ykgL"
                     /locus_tag="ACHJ12_003958"
     CDS             complement(1688..1915)
                     /gene="ykgL"
                     /locus_tag="ACHJ12_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO4991674.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            2738..3280
                     /gene="ecpR"
                     /locus_tag="ACHJ12_003959"
                     /gene_synonym="matA"
     CDS             2738..3280
                     /gene="ecpR"
                     /locus_tag="ACHJ12_003959"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO4991675.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            3355..3942
                     /gene="ecpA"
                     /locus_tag="ACHJ12_003960"
                     /gene_synonym="matB"
     CDS             3355..3942
                     /gene="ecpA"
                     /locus_tag="ACHJ12_003960"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO4991676.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            4000..4668
                     /gene="ecpB"
                     /locus_tag="ACHJ12_003961"
                     /gene_synonym="yagY"
     CDS             4000..4668
                     /gene="ecpB"
                     /locus_tag="ACHJ12_003961"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO4991677.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            4694..7219
                     /gene="ecpC"
                     /locus_tag="ACHJ12_003962"
                     /gene_synonym="yagX"
     CDS             4694..7219
                     /gene="ecpC"
                     /locus_tag="ACHJ12_003962"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO4991678.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            7209..8852
                     /gene="ecpD"
                     /locus_tag="ACHJ12_003963"
     CDS             7209..8852
                     /gene="ecpD"
                     /locus_tag="ACHJ12_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO4991679.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGAGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLLIKPRDFSISIISDEYT
                     QTPSREGYVGSGEPALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8821..9531
                     /gene="ecpE"
                     /locus_tag="ACHJ12_003964"
     CDS             8821..9531
                     /gene="ecpE"
                     /locus_tag="ACHJ12_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO4991680.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVRQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9844..10173
                     /gene="ykgJ"
                     /locus_tag="ACHJ12_003965"
     CDS             9844..10173
                     /gene="ykgJ"
                     /locus_tag="ACHJ12_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4991681.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(10421..11035)
                     /gene="yagU"
                     /locus_tag="ACHJ12_003966"
     CDS             complement(10421..11035)
                     /gene="yagU"
                     /locus_tag="ACHJ12_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4991682.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            11453..12142
                     /gene="paoA"
                     /locus_tag="ACHJ12_003967"
     CDS             11453..12142
                     /gene="paoA"
                     /locus_tag="ACHJ12_003967"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO4991683.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            12139..13095
                     /gene="paoB"
                     /locus_tag="ACHJ12_003968"
     CDS             12139..13095
                     /gene="paoB"
                     /locus_tag="ACHJ12_003968"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO4991684.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            13092..>13911
                     /gene="paoC"
                     /locus_tag="ACHJ12_003969"
     CDS             13092..>13911
                     /gene="paoC"
                     /locus_tag="ACHJ12_003969"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4991685.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRP"
BASE COUNT         3696 a         3554 c         3404 g         3257 t
ORIGIN      
        1 tattggaaat acgtcgaagt aggtatatac gccagaataa atcgatatat tttttatgga
       61 tattcatttt ccttcatcgg actgtaaatt gcggagattt cgaacttgca ctacccatcg
      121 caacactcaa acagatttat gaatttcgtt cataacccct gacaaatcct gccgtctaat
      181 cgagcttttc cctttacgtt atgcttctca tgaacgagaa cacaacttat tcatgaatta
      241 accattccgg ataaactatg gaatcaaatt caggcatcga aaggaactgg ctggtttccg
      301 ttaatagatt aaatatctat tactctcccg agagtaaaac tatcaaatca atgagtaacc
      361 gatacagcct cacaaattct ttcagataat taacccgtca ctgcggcacg ttctctcata
      421 aggcgtgcct gcagtgtaat ccagtatgta tccggcatag ttgacggttt aatagaactc
      481 aattcctttc ccaggttacc aacaacgata tcttgtataa caccccagga gatacgacat
      541 tatgatgaaa tgaggtatgc tcctcagcgc caatcaacgc ccggactggg ccagtcttga
      601 gatcagactc attcccggaa ctgacttcag gcgacatata tcaatgacgt tggtgcaata
      661 aaagatagcc gatgtaatac atctattcaa atttgtgata catgtcaaaa aattggttga
      721 ccaaactcgt tatttatata agggcactta cgaagtgcgc tcttttttaa agcgaggaag
      781 taccaatgaa agagaataaa gtacaacaaa tcagtcataa actgattaat atcgttgttt
      841 ttgtcgcaat tgtagaatac gcctatttat ttctccattt ctattaataa cggcaataaa
      901 ctgttcactt cagtgatatt taaaatatgc atcctctccc tttttgtaag taattattat
      961 atccgtggga gaggaataca cgctgtcagg taatcaatca tactgcgata aatcatcggc
     1021 cagtaaagtg gagataacct ccattgtcga aaaatccata ctctcagcaa aaccatcatc
     1081 aatcactcat ccaggcgttt atgggagcgt caccaatggc tgctaacaat gccagagttc
     1141 cccgttgcga aaattccaca tccacaaaga gtcacaggga ttgggtgttg aaatgatccg
     1201 gatgagcatg tatctttatg gttatgttat aacataacag gtaaaaatga tgaagcccaa
     1261 tatccatcct gagtatcgta ctgtggtgtt ccacgacacc agtgttgatg agtactttaa
     1321 aatcggctcg actatcaaaa cagaccgtga gattgagctg gatggcgtaa cgtatccata
     1381 cgtgacaatt gatgtctctt ctaaatcgca cccgttctat acagggaagc tgagaacagt
     1441 ggcatcagaa ggaaatgttg cacgattcac ccaacgtttt ggtcgttttg ttagcacgaa
     1501 aaaggggtcg tgatgaaagt tcttaactct ctgcgtaccg caaaagaacg ccatccagac
     1561 tgtcagattg tgaagcgaaa aggacggcta tatgtgattt gtaaatctaa tccacgtttt
     1621 aaggccgttc agggtcgtaa gaaaaaacgt tgattcaaaa ttcgacggat taacgatatt
     1681 tgtctgatta ataatcagat cggattaatg ttggtgtgtt tataacacca acattaattt
     1741 tcctggggat atattcctcc tgttcatttg aggccaactg cctgacgttt ctctccgaat
     1801 attccattat cttaatgttg acttgttgac cagcttcgcc cctgtatgct ggcatcaact
     1861 ctcttttaga ctgaacacgc cactcagtct cctccctttg cggcgcagcc tgcattttca
     1921 ctcaaactgt taagatgata aatgtggtaa atctgttggt actaacataa aaacgtttac
     1981 gccacaggaa cagtctgatc caccggtaac cccgtcgccg acgttcgagt gccagttaga
     2041 gtaacgcaca cagataactg cactcagtgc cctgacaaag aggccatcgt tcctgtgaca
     2101 gctggcagcc ttcgtttaac ttcacttaat ctggctcttg ggggcttacc gaacagatga
     2161 cgtacatacg cccgttcaat tttccatcac ttattgtaat gaacacctat aaccattttg
     2221 tgcggcatgt taatccatta aaatttctta ctgattggca aatcatcttc aatgacagct
     2281 cattatagtt ttatattcta tcccttaccc ttaaaacttg tttttttact agtccatcac
     2341 acagcgcatt aagactattc ctaacacttc agggcaaagt tcctgaccaa tataaaatgc
     2401 aagtaagaat tgaacgttat attgccaata accttatgaa accaaatgtc tttttcttct
     2461 tatcaaaaaa gcaatatttt cagtttttct aaatattgac ttaaccattg aattcctttt
     2521 ccgttcacat attgacactc atcgggaaaa aaacataaat ttaagcccaa tcgaaaataa
     2581 ttaaacttaa tctcgtttaa cctttattga tatgtactac gtatcttatt tacttccggt
     2641 ttactaagga aactgaatgc acctgtaaaa attacaggtt tggaaagtag tgacatggca
     2701 aagtgattac agcagggact atgaggttaa aaaccatatg gaatgtcaaa accgttctga
     2761 taaatacatc tggtctcccc atgacgccta cttctataaa ggactatctg aactgattgt
     2821 ggatatcgac agattaattt atctatcgtt ggagaaaatt agaaaagatt tcgtgtttat
     2881 caatctcaat acggattctt tatctgaatt tataaaccgt gataatgaat ggttatccgc
     2941 ggtaaagggg aaacaggtcg tattgattgc ggccagaaag tcagaagcct tagcaaatta
     3001 ttggtattac aacagcaata ttaggggcgt ggtatacgca ggactgagtc gtgatattag
     3061 aaaagaactg gcctatgtga ttaatggcag gttcctgaga aaagatatta agaaagataa
     3121 aatcacggac cgggaaatgg aaattatccg catgacggcc cagggaatgc aacctaaatc
     3181 gattgccaga attgaaaatt gtagtgtgaa gacagtgtat acccatcggc gtaatgctga
     3241 ggccaagctg tactcaaaaa tatataagtt ggttcagtaa actccaggca agttagtttt
     3301 aaaaaatgac tcactgggac atcacgtcct caattcaact cgggaagaaa tgcaatgaaa
     3361 aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gcatgggtgt ggcgcaggct
     3421 gctgacgtaa cagctcaggc tgtagcgacc tggtcggcaa cagccaaaaa agacaccacc
     3481 agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc agtatgccga aggcattaaa
     3541 ggttttaact cacagaaagg tctatttgac gtggctatcg agggtgactc aacggctacc
     3601 gcctttaaac tgacctcacg tcttatcacc aacacattaa cccagttgga tacctcaggt
     3661 tccacactga atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac tggcgatacc
     3721 gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccgct ggctaacggt
     3781 tacaatgcca gcaatcgtac caccgcacag gatggtttca ccttctccat catcagcggt
     3841 accaccaatg gtaccaccgc agtaacagat tacagcactc taccggaagg catctggagc
     3901 ggcgacgtta gcgtacagtt cgacgcgacc tggaccagtt aatctctctg atgtaccagc
     3961 aggggtagcc cccctgcttt attcctggac ggaattgtta tgaaaaagca ccttctgcct
     4021 ctcgctctgc tgttttccgg aatatctccg gcccaggcgc tggatgtcgg cgatatatca
     4081 tcgtttatga acagtgacag cagcacgctg agcaaaacga tcaaaaacag taccgacagt
     4141 ggtcgcctta tcaatatccg tctcgaacgg ctctcttcac cgcttgacga cgggcaggtt
     4201 atctcaatgg acaagccgga tgagttgcta ctcactcccg ccagcttgct gctacccgcc
     4261 caagccagcg aagtgatccg cttcttctat aagggacccg cagatgaaaa agagcgctac
     4321 taccgcattg tctggtttga tcaggccctc agtgatgcgc agcgcgataa tgccaaccgc
     4381 agcgctgtgg ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc cccccgccag
     4441 gcgaactacc actttcagta cgccaacggc tccctgacaa atacaggaaa tgcgacgctg
     4501 cggatcctcg cctacggccc ttgcctgaaa gccgccaacg gtaaggagtg taaagagaat
     4561 tactacctga tgccgggcaa gtcgcgtcgt tttacccgcg tggacacggc ggataacaaa
     4621 ggacgggttg cactttggca gggtgataag ttcattcccg tgaaatagat agctgtgcag
     4681 atggataacg acaatgcctt tacgacggtt ctccccagga ctgaaagccc agtttgcctt
     4741 cggcatggtc tttttgttcg ttcagcccga tgccagcgct gctgacataa gtgcgcagca
     4801 aataggtggg gtgattattc cgcaggcctt cagtcaggcg cttcaggacg gcatgagcgt
     4861 cccgctctat attcatctcg ccggtagcca gggtcgccag gacgatcagc gaatcggcag
     4921 cgcttttatc tggctggatg atggacagct acgcatccgg aaaatacagc tggaagagag
     4981 tgaagataac gccagtgtca gcgaacaaac tcgacagcag ctgatggctc tggccaacgc
     5041 cccgttcaat gaggccctta ccatccccct gactgacaac gcgcagctgg atctcagctt
     5101 gcgccaactg ctgctgcagc tggtggtcaa gcgcgaagcg ctgggcaccg tactacgctc
     5161 acgtagcgaa gacatcgggc agtccagtgt taacaccctc agcagtaatc tgagctataa
     5221 cttgggcgtc tataacaacc agttgcgtaa cggcgggagc aacacatcca gctatctgtc
     5281 gctgaataac gttactgcgc tgcgcgaaca ccatgtggtg ctcgacggct cgctgtacgg
     5341 gatcggtagc ggtcaacagg acagtgaatt atataaagcg atgtatgaac gcgattttgc
     5401 cggtcaccga tttgccggtg gaatgctcga cacctggaac ttgcagtcct tagggccgat
     5461 gaccgccatt tcagcaggga agatttacgg cctttcctgg ggaaaccagg ccagctccac
     5521 catcttcgac agcagccagt cagccacgcc agtgatcgcc tttttaccgg cggcgggcga
     5581 agtacatctc acccgtgatg ggcggctact aagcgttcag aacttcacta tgggcaatca
     5641 tgaagtggat acccggggtc taccgtacgg gatttacgat gtggaagttg aggtgatcgt
     5701 taacggtcgc gtgatcagca aacgccccca gcgggtcaat aagctgttta gccgggggcg
     5761 cggcgtcggt gcaccactgg cgtggcaggt atggggcggt agctttcata tggatcgctg
     5821 gtcggaaaac gggaaaaaga cgcgaccagc taaagagagt tggctagcag gtgcctcgac
     5881 ctccggctca ttgagtacgc ttagctgggc ggcaacggga tatggatacg ataatcaggc
     5941 ggtgggtgaa acccgtctga cgctgccgct tgggggggcg atcaacgtta acctgcaaaa
     6001 tatgctggcc agtgacagct catggagcag catcggcagc atcagcgcca ctctaccggg
     6061 aggctttagt tcgctgtggg ttaaccagga aaaaacccgc attggcaatc aattgcgacg
     6121 tagcgatgcc gacaaccgtg caatcggcgg cacactcaac ctgaactcac tgtggtcgaa
     6181 gctgggcaca ttcagcatca gctacaatga tgaccgccgt tacaacagcc attattacac
     6241 ggcagattac tatcaaaatg tctacagcgg tacctttggt tcgcttggcc tgcgggccgg
     6301 tattcagcgc tataacaacg gcgacagcaa cgccaataca gggaaatata tcgctctcga
     6361 tctctcgcta ccactgggca actggtttag cgcagggatg acccatcaaa acggctacac
     6421 catggcaaac ctgtcagcac gcaaacagtt tgatgaagga accattcgca ctgttggtgc
     6481 caatctgtca cgtgccatct ccggcgatac cggtgatgac aaaaccctca gcggtggggc
     6541 gtatgcacag ttcgacgctc gttacgccag cggaacgctg aacgtcaata gcgcggcgga
     6601 cggctacatc aataccaact tgaccgccaa tggcagcgtc ggctggcagg gtaaaaacat
     6661 cgctgccagc gggcggactg atggcaacgc tggggtgata ttcaacaccg ggctggagga
     6721 cgacggtcag atcagcgcca aaatcaacgg gcggattttc ccgcttaacg gcaagcgtaa
     6781 ctatctcccg ctctctccct atggaagata tgaggtggag ttacagaaca gcaaaaactc
     6841 actcgacagc tacgatatcg tcagcggccg caaaagccat ctgactctct atccaggcaa
     6901 tgtcgctgtc attgagccag aggtgaagca gatggttacc gtctccggtc gtatccgtgc
     6961 ggaagacggc acactgctgg ctaacgcacg gattaacaac catatcggcc gaacccgaac
     7021 cgatgaaaac ggcgagtttg tcatggacgt ggataagaaa tatcccacta tcgattttcg
     7081 ctacagtggc aataaaacct gcgaagtggc actggaactc aaccaggcgc gcggtgccgt
     7141 ctgggtcggt gatgtggtct gcagcggcct ctcatcgtgg gcggcggtga cgcagacagg
     7201 agaagagaat gagagttaac ctactaataa cgatgataat tttcgcgcta atctggccag
     7261 taactgagct cagagcggca gtgagcaaaa caacctgggc ggatgcaccg gcacgcgagt
     7321 ttgtgtttgt cgaaaacaac tcagacgaca actttttcgt cacgcctggc ggggcgctgg
     7381 atccgcgcct gaccggtgcc aaccgctgga ccggtttaaa atacaatggt gcaggaacca
     7441 tctatcagca aagcctcggc tacattgata acggttacaa caccggcctc tataccaact
     7501 ggaagtttga tatgtggctg gaaaattcgc cggtttcatc tcctttaact ggcttacgct
     7561 gcatcaactg gtacgcaggg tgtaatatga ccaccagtct tatcctgccg caaaccaccg
     7621 acgccagtgg attttatggc gcgacggtga ccagcggcgg cgcgaagtgg atgcacggca
     7681 tgttgtcaga cgcgttttac cagtatatgc aacaaatgcc cgtcggcagc agctttacaa
     7741 tgaccatcaa tgcctgccag acctctgtga actatgacgc cagcagcggc gcacgctgta
     7801 aggatcaggc ctccggcaac tggtatgttc gcaacgtcac ccatacgaaa gcagcaaatc
     7861 tgcggttaat aaatacccac tctctggcgg aggtatttat caacagcgac ggagtaccga
     7921 ctctgggcga agggaacgcc gactgccgga cacagaccat cggcagcctt tcaggattaa
     7981 gttgtaagat ggtcaactat accctgcaaa caaacggact cagcaacacc tcaatccata
     8041 tattcccggc gatcgccaac tcgtcgttag cctcggccgt cggggcgtac gatatgcagt
     8101 tcagtctgaa tggcagttca tggaaaccgg tgagcaatac cgcctattac tacaccttca
     8161 acgagatgaa gagcgcagac tcgatctatg ttttcttctc gagcaacttc tttaagcaga
     8221 tggtgaacct ggggatcagc gacatcaaca ccaaggatct gttcaacttt cgctttcaga
     8281 acacgacatc accggagtct ggctggtatg aattttctac ctccaacacg ctgcttatca
     8341 aaccccgtga tttcagcatc agtattatct ccgatgaata tactcagaca ccgtcgcggg
     8401 agggatatgt cggcagcggc gagccggcac tcgatttcgg ctatatcgta accaccagcg
     8461 gtaaaacagc tgccgacgaa gtgctgatca aggtgaccgg acccgcgcag gtgattggcg
     8521 ggcgctccta ttgtgtcttc agctccgatg acggtaaggc gaaagtaccg ttcccggcga
     8581 cgctttcctt tattacccgc aacggagcta caaaaaccta cgatgccggg tgcgatgata
     8641 gctggcggga tatgaccgat gcgctgtggc taaccacacc gtggactgat atctctggcg
     8701 aagtggggca gatggataag accacagtca aattttcgat tccaatggat aacgccattt
     8761 cgctgcgtac ggtagatgat aacggctggt ttggcgaagt cagcgcttca ggagaaattc
     8821 atgttcaggc gacgtggcgt aacattaact aaggccctgc tgacagcggt ctgtatgctg
     8881 gcggcacctt tgacacaggc gatttcggtc ggcaatctga cattttcgct gccgtccgag
     8941 actgactttg tcagcaaacg tgtagtgaat aacaacaaaa gcgcacggat ataccgtatt
     9001 gccatcagtg ctattgatag cccgggcagc agtgaattgc gcacccgacc ggtggatggt
     9061 gaactgcttt tcgccccccg ccagctggcg ttgcaggctg gtgagagcga gtattttaaa
     9121 ttttactatc atgggccacg ggataaccgc gagcgctact accgggtctc atttcgcgag
     9181 gtccccactc gtaaccagac aagacgcagc ccaaccggcg gcgaggtcag cacggagccg
     9241 gtggtggtga tggataccat tctggtagta cgaccacgtc aggttcagtt taaatggtcc
     9301 ttcgacaagg tgacaggaac agtaagtaac actggcaaca catggtttaa gctactgatt
     9361 aagccaggat gtgattcgac cgaagaggaa ggcgatgcct ggtatctacg tccgggagac
     9421 gtggttcgtc agcctgagtt acgtcagccg gggaatcatt atctggtcta taacgacaaa
     9481 ttcattaaga ttagcgattc ctgtccggct aagccccctt cggcggacta agacttatcc
     9541 atcggtcgag ggaagaattc cacctcagag ctccaaaaat cgtttaaatg atgtggaagc
     9601 gatcgtcaat gtcacctgta acgatgggct ttctcatatg cacaccatag tgttcaggag
     9661 acacggaaag atatcaggca gcctgctaac gagtggttta atcacgaatt agtacgtaaa
     9721 atcggtaacg gctggaaatc attcaatact cacactatcg aaagttcccc agccaaccgc
     9781 ggtacgttct tacatacgat gtaccgctgt tctctttacg atttatagct gtactggtga
     9841 attatgagca atctgaatcc atgcatgacg tgtggtgcct gttgtgcatt tttccgcgtc
     9901 tctttttact gggccgaagc cgacgatgct ggcggtacca ttcccgccag gctcactgaa
     9961 caaatatccc cttttcaccg atgcatgagc ggtaccaatc agaaaaaccc ccgatgtatt
    10021 gcccttgcag gaaccccggg caaaaatgcc tgctgcacga tatataaaaa tcgatcgtcc
    10081 acatgcagag aattcgccat gtctggtgag aacggagtcg tcaatgaggc ttgcaatcgt
    10141 gcaagggcta aatacgggct gacaccgtta taaacataca ataattaatt gcactgcccc
    10201 gccagcgata atagcggggc ttcgtttttc aggggtaaca aaacccgata cttcttctat
    10261 ttgccagcaa caatgcctct cttctgtagc gttcttcgga tctacatcat cctgaagtag
    10321 cgcgatatac tcactgacta tctgaatcag aatataaaaa gcaatgtttt taacctataa
    10381 aaatggcgct gtatttgcgc catttttatc attcaatgca ttatctgttt gagcctaaag
    10441 ggatctcagg gtctggctca tgagtaattc tgtttcgtaa atctctgcga ataatttcaa
    10501 tagaccagaa ccagactaaa tgtccaaaaa tttcagaaac attctcatac cacgggagat
    10561 caaacagagg tggcgtcagt cccatgagag ggaatgaaat catatgaaca aaaagttggg
    10621 ctaaagcacc tgccagtaag ccctgccaga gtttaatttt tggaaatact tcagcgacca
    10681 cacaataacc gacagcaaac actatcgaaa agataatgtg cgtaacacca acccagttaa
    10741 agacatgccc ggcaaaggta taaacagccg cattgggatc tgtcagcccc aaccagtctc
    10801 gaagaaaaat atacggtgga ttgagaaaat tacgcgagca atcaatttgg cctgcagccc
    10861 tgattaatga ttccgggcca cacgctgcat taaacatatc caccgggcta cgtggcggca
    10921 atggaacttc agccccccac ttcacgaatg cggaaacaac gccagcaatc agcccaatga
    10981 atgcagcaag accataacgt ctgcggttcg gtggagtttg ttcaaatata ttcatatcta
    11041 ccctgcttgt accattatgt tatacacctc ttcaggagta ttcataaaac aaggcaaatg
    11101 taaagaactg tattgttttg tataacaaga tggtttccta atcgccaatg aatataagct
    11161 ccatcatttc tccctatttt tatattagaa gtgacagaga tttgcagggt gatgcagagc
    11221 tgaaaatcac aggtttcctt attggttttt gcatcgtaca actaaagcaa taaaccagct
    11281 cccatcatca tcaacatccc ggcgaacatg aatttattag ccgggctcat agctgcattc
    11341 gcgttttaag aataatcctc ctgctgtcgc cgactatgct taacatttaa aaaagcatca
    11401 gcactctcgc aacgcactct tattttcccc tttagaatac cggaggcctg gtatgagcaa
    11461 ccaaggcgaa taccccgaag ataatcgggt tgggaagcac gagccgcacg atttaagttt
    11521 gacccgtcgc gatctgatta aagtgagcgc cgcaacagcg gcgaccgccg tggtttatcc
    11581 tcattctacg ctggcggcaa gcgttccggc agctacaccc gcgccagaga taatgcccct
    11641 gacactgaag gtgaacggca aaaccgagca gcttgaggtg gatacccgaa ccacgctact
    11701 ggacgctttg cgtgaaaatc tgcatttgat cggtaccaag aaaggttgcg atcacggaca
    11761 gtgcggagcc tgtaccgtgc tggtcaatgg tcgcaggctt aatgcctgcc tgacgcttgc
    11821 agtcatgcat cagggggccg agatcaccac cattgaaggc ctgggttcgc cagataatct
    11881 tcaccccatg caggcggcct ttatcaagca tgatggcttc cagtgcggct actgcacctc
    11941 cgggcaaatt tgctcatcag tagcggtgct aaaagagatt caggacggca ttcccagtca
    12001 cgtcacggtc gatttggttt ccgctccaga aacaactgcc gatgagatcc gtgaacgtat
    12061 gagcggcaac atctgtcgct gtggtgcata cgctaacatc cttgccgcca ttgaagatgc
    12121 tgcgggggag ataaaatcat gaaggcgttt acctatgaac gagtgaacac cccagccgag
    12181 gcggcactta gcgctcagcg cgtacccggc gcaaaattta tcgcgggcgg gaccaatctg
    12241 ctggacctga tgaagctgga aattgaaacg cccacccacc ttatcgatgt gaacggcctc
    12301 gggctcgata agattgaagt gaccgacgcg ggtgggctgc gcatcggcgc actggtacgg
    12361 aacaccgacc tggcggctca cgagcgcgtg cgtcgtgatt acgcggtact ctcccgcgcc
    12421 ctgctcgctg gcgcatctgg tcagttacgt aatcaggcaa ccaccgcagg taatctgctc
    12481 cagcgcacgc gctgccccta tttttacgac accaatcagc cctgcaataa gcgcctgccc
    12541 gggagcggct gcgcggcgct tgaaggcttt agccgtcagc acgcggtggt aggcgtaagt
    12601 gaagcctgca tagccaccca tccgagcgat atggcggtcg caatgcggtt gctggatgcg
    12661 gtggtggaaa ccatcacgcc ggagggaaag actcgcagta tcacactggc tgatttttat
    12721 cacccaccgg ggaaaacgcc gcacattgaa accgccctgc ttcccggtga gcttatcgtt
    12781 gcggtgacgt tacctccacc gctcggcgga aaacatatct accgtaaggt gcgcgatcgc
    12841 gcctcctacg cctttgccct ggtatcggtc gcggcgatta ttcagcctga cggcagcggg
    12901 cgcgtcgcgc tgggcggagt agcacataag ccctggcgca ttgaggctgc ggatgctcag
    12961 ctatcccagg gggcgcaggc cgtatatgac acgctgttcg ccagcgccca tcccaccgct
    13021 gaaaacacct ttaaactcct gttggcgaag cgaacgcttg cctccgtact ggctgaagcg
    13081 agggcacagg catgaaattt gataaacccg caggggaaaa cccgatcgat cagctgaagg
    13141 ttgtcggtcg tccccatgac cgcatcgacg gaccgctgaa aactaccggc acggcacgct
    13201 acgcctacga atggcatgaa gaggccccca acgccgccta tggctatatc gtcggttccg
    13261 ccattgccaa aggacgcctc accgcccttg atacggacgc cgcgcaaaaa gcgccgggcg
    13321 tactggctgt cattaccgcc agtaacgccg gggcactcgg caaaggcgac aaaaacaccg
    13381 ccaggctgtt aggcggcccc actattgagc actatcatca ggccattgcg ctggtagtgg
    13441 ccgagacctt cgaacaggcg cgagcggcgg cctcgctggt gcaggcacac tatcgccgta
    13501 ataaaggagc ttactccctg gcggacgaaa aacaggccgt caatcagccg ccggaagaca
    13561 cgcccgacaa aaacgtcggt gactttgacg gggctttcac ctccgctgcg gtgaagattg
    13621 atgctaccta cacgaccccg gaccagagcc atatggcgat ggagccgcat gcctcgatgg
    13681 ccgtctggga tggaaataag cttaccctct ggacctcaaa tcagatgatt gactggtgcc
    13741 gcaccgatct ggcaaaaacg ctgaaagttc ccgtggagaa tgtgcgtatt atctccccgt
    13801 atatcggcgg agggtttggc ggcaagctgt tcctgagaag cgatgcgctg ctggcggccc
    13861 tcgccgcccg agcggtgaaa cgtccggtta aagtgatgct cccccgcccc t
//
LOCUS       DAZFFI010000114        13774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFFI010000114 DAZFFI010000000
VERSION     DAZFFI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.114"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..910
                     /locus_tag="ACHJ12_003970"
     CDS             41..910
                     /locus_tag="ACHJ12_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4991686.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1113..1466)
                     /locus_tag="ACHJ12_003971"
     CDS             complement(1113..1466)
                     /locus_tag="ACHJ12_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4991687.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1604..3250)
                     /gene="groL"
                     /locus_tag="ACHJ12_003972"
     CDS             complement(1604..3250)
                     /gene="groL"
                     /locus_tag="ACHJ12_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4991688.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3294..3587)
                     /locus_tag="ACHJ12_003973"
     CDS             complement(3294..3587)
                     /locus_tag="ACHJ12_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4991689.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3864..5120
                     /gene="yjeH"
                     /locus_tag="ACHJ12_003974"
     CDS             3864..5120
                     /gene="yjeH"
                     /locus_tag="ACHJ12_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4991690.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWVGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5136..5612)
                     /locus_tag="ACHJ12_003975"
     CDS             complement(5136..5612)
                     /locus_tag="ACHJ12_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4991691.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5949..7385
                     /gene="aspA"
                     /locus_tag="ACHJ12_003976"
     CDS             5949..7385
                     /gene="aspA"
                     /locus_tag="ACHJ12_003976"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4991692.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7503..8804
                     /gene="dcuA"
                     /locus_tag="ACHJ12_003977"
     CDS             7503..8804
                     /gene="dcuA"
                     /locus_tag="ACHJ12_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4991693.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8920..9258
                     /gene="cutA"
                     /locus_tag="ACHJ12_003978"
     CDS             8920..9258
                     /gene="cutA"
                     /locus_tag="ACHJ12_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4991694.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9234..10931
                     /gene="dsbD"
                     /locus_tag="ACHJ12_003979"
     CDS             9234..10931
                     /gene="dsbD"
                     /locus_tag="ACHJ12_003979"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4991695.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10968..11543
                     /locus_tag="ACHJ12_003980"
     CDS             10968..11543
                     /locus_tag="ACHJ12_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4991696.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11650..11725
                     /locus_tag="ACHJ12_003981"
     tRNA            11650..11725
                     /locus_tag="ACHJ12_003981"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11683..11685,aa:Phe,seq:gaa)
     gene            complement(11921..12958)
                     /locus_tag="ACHJ12_003982"
     CDS             complement(11921..12958)
                     /locus_tag="ACHJ12_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4991697.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(13026..>13774)
                     /locus_tag="ACHJ12_003983"
     CDS             complement(13026..>13774)
                     /locus_tag="ACHJ12_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFO4991698.1"
                     /translation="AAGDVAEVPVAEVVPVLLQSAAISAESVIQPLPVVQSPVEDAEN
                     EGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPDKESRIELPEESGC
                     VTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHVVSG
                     YVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEHSGR
                     YKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
BASE COUNT         3309 a         3637 c         3316 g         3512 t
ORIGIN      
        1 aattaattat ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg
       61 tgttaatacc ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa
      121 agaacctcgc aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct
      181 gattgcgctg gaaagtcgta tgcatcaaaa gcataaactg gatgctgctg cacgccttca
      241 gtatgagcaa gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct
      301 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
      361 taatcaaggc gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt
      421 agtcgtcaat gagttgcaga ttgaaatgct ggcgcgagca atcattcatg caattaataa
      481 tgcggaaatg cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata
      541 tgacgttgac tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg
      601 gaaacataac ctgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
      661 caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat
      721 agaggcgatc actcgccgaa tgcttgattt cagccaacga ctgaaaaaac tcgcgggtgt
      781 gccatgtcag gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga
      841 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
      901 ggcgaaataa tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga
      961 taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct
     1021 caagcattca atcagttgca atgcctgctg taggcctgat aagcgaagcg catcaggcaa
     1081 tgatacgttt gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg
     1141 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
     1201 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
     1261 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
     1321 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
     1381 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
     1441 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
     1501 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
     1561 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
     1621 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatca tttttcggca
     1681 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
     1741 gagcagaacg ggttactttg gttgggtcca ggatacccat gtcgatcatg ttgccgtatt
     1801 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacggtg ttagcaacaa
     1861 cagacggttc ttcgccgcag ttcaggacga tctgacgcag cggagcttcc attgcacgca
     1921 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
     1981 acgctacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
     2041 gggtcgcgtg cagggcgtct tcaacgcgtg cttttttctc tttcatttca acttcggtag
     2101 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgt tcctgcagtt
     2161 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
     2221 cctggattgc agcttcttca cccacgccat cgatgatagt ggtggtgtct ttgttgatca
     2281 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct
     2341 cttcagagat tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacgac
     2401 gatcgccgaa gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa
     2461 caaccagagt tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttac
     2521 ctgctttagc aacggcttcc agaaccggca gcatttcgcg gatgttagag attttcttgt
     2581 cagccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
     2641 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
     2701 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
     2761 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
     2821 tcgctttaga gtcagagcac ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
     2881 ccgctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca acagctttca
     2941 gaccttcagt gatgatagcc tgagccagta cggttgcagt ggtggtaccg tcgcctgcag
     3001 cgtcgttcgc tttggaggca acttctttca ccatctgcgc acccatattt tcgaacttgt
     3061 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
     3121 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
     3181 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
     3241 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
     3301 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
     3361 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
     3421 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
     3481 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
     3541 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
     3601 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
     3661 accagagaaa tggggatgag gcttcgcccc ttcaaggggg aaaaagaaaa aaaattctga
     3721 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
     3781 accgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
     3841 gggcgcaatc ggatatcaaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
     3901 gcattggcct gctatcgacg tcattattag gcactggcgt gtttgccgtt cctgcgttag
     3961 ctgcactggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
     4021 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggcgtcg
     4081 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggctgtttt
     4141 tatcagtcat tcccgtgggt ttacccgccg cactacaaat tgctgccggg ttcggccagg
     4201 cgatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
     4261 tgtggtatat cggtactcgc ggtgccagtt ccagtgccaa tctacaaacc gttattgccg
     4321 gacttatcgt cgcgctgatt gtcgctatct ggtgggtggg cgatatcaaa cctgcgaata
     4381 tccccttccc ggcacctggt aatatcgaac ttaccgggct atttgctgcg ttatcagtga
     4441 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
     4501 cagagcgtga ttttcctcgt gctttgatga ttggtctgct gctggcagga ttagtctact
     4561 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gcggcggcag
     4621 catcgcttcc aaaaattgta gtgcagttgt tcggtgtcgg agcgttatgg attgcctgcg
     4681 taattggcta tctggcctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
     4741 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
     4801 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc acgttggtga
     4861 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
     4921 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
     4981 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
     5041 tctatgcgct gatcatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
     5101 aaaatggcat aaccacataa tccggcgttt cgacattaat cctggcgatc gtctttatga
     5161 tcaaggcggt cgcgctcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
     5221 ccggtcccgg cgctaaaacc gccgcctggc atacgagaaa agcgcaaatg cggcatcaac
     5281 ttcactgtca gatgcttttg caccggcggt aataaaagca gaaggccgag gaagtcggtg
     5341 aaaaagcccg gtaataaaag cagcaaaccg gcaatgatca gcgaaacact tttaatcatc
     5401 tccgccgctg ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
     5461 ccctggttac gtaccagtga catcccgata actgacgtga atataaccag cacgagggtc
     5521 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
     5581 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtaa
     5641 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
     5701 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
     5761 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
     5821 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
     5881 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
     5941 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
     6001 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
     6061 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
     6121 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
     6181 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
     6241 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
     6301 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
     6361 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
     6421 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
     6481 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
     6541 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaata
     6601 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
     6661 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
     6721 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
     6781 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
     6841 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
     6901 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
     6961 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
     7021 agctgcagtt gaacgttatg gagccggtca ttggccaggc catgttcgaa tccgttcaca
     7081 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
     7141 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
     7201 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
     7261 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
     7321 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
     7381 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
     7441 gtgagcagta acttaaaaat aacaacctaa tatcaacttg ttaaaaaaca aggaaggcta
     7501 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
     7561 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
     7621 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
     7681 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
     7741 tgcgccgtaa cccgaaatac atcacgatcc tcgcaccgat cgtgacctat ttcctgacta
     7801 tctttgctgg tactggcaac atatctctgg cgacactgcc agttatcgct gaagttgcga
     7861 aggaacaagg cgtcaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
     7921 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
     7981 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
     8041 tggtgatgtc ctttctggtc actatgctgt tcaactccaa actctctgac gatccgattt
     8101 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
     8161 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
     8221 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
     8281 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
     8341 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
     8401 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
     8461 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
     8521 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actaatgccg atggctctgg
     8581 cactgaacgt ttcaccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
     8641 ttctgccgac ctacccgacg ctggttgctg cggtacagat ggatgacacg ggtactaccc
     8701 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
     8761 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgatca atcgcggggc
     8821 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtacgt tgtatagtga
     8881 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
     8941 aataccgcgt ctgtcgtggt gctatgtacc gcaccagatg aagcgacagc ccaggattta
     9001 gctgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
     9061 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
     9121 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
     9181 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
     9241 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
     9301 acgcgccggg acgttcacaa tttgtccccg cggatcaagc ctttactttt gattttcagc
     9361 aaaaccaaca tgaccttaat ctgacctggc agatcaaaga cggttactac ctctaccgta
     9421 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
     9481 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
     9541 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
     9601 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
     9661 ccaacaacgc agcgtcacag cctgtgtctg tttcgcagca agagcagcac accgcgcaat
     9721 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
     9781 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
     9841 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
     9901 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
     9961 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    10021 ttactctaca actcccctct tcgctgcaaa cacgtctcac gttgatgagc aatcgccaac
    10081 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    10141 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    10201 ggctgggcgg cggcacgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    10261 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    10321 ccgcgtttgg ttttgtgatc ctcgcactgc cggtcttcct gctggagcga gtgattggtg
    10381 atgtatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    10441 tcaccagcct acaggctaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    10501 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    10561 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    10621 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    10681 gtaaagagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    10741 cggtcttact tcaggccaac gtcacggcca acgacgcaca agatgtggcg ctgttaaagc
    10801 atcttaatgt ccttggccta ccgacaattc tcttttttga cggacaaggc caggagcatc
    10861 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    10921 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    10981 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    11041 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    11101 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    11161 gtcagctgat gctggacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    11221 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    11281 cgttttatcc cgatcctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    11341 cctacgattt cacccacgaa ctgttaacca cactggaagt tgacgatccg gcgatggtag
    11401 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    11461 aggcggatgt cgacactgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    11521 gccagggtgg tgcattgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    11581 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    11641 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcggt
    11701 ggttcgattc cgcctccggg caccatatac cagaacacgc tggttaaggg atgagatcct
    11761 tgacgggcgg ttaatacagg atgcagcatc aaagggtctc cgtttgtgta agttgtggtt
    11821 acactgattg agttcggtat cgtatcctca aacagggtgt cttttgcgga gacgccctgt
    11881 tctcgttaac actgtatgct tacatcgctg attcagcctg tcaagattta ttcccccctt
    11941 tgcaaatctt gctcactgtg cgctcatctg tatacaaact cagggcatct tccagtgtct
    12001 gtgctgcaga ctccatacag acatcaacat accccatggt cgttgtaaga ctcctgtgtc
    12061 ccagtatgga tttgaccaga tgtaaattac gttcaggata tttcatcagt ctggttgcca
    12121 gcgtatgtct gaaacgatgt ggtgaaacag gaaaaccaca ttctcgcgaa agtcgccgga
    12181 aaaatgaacg tacggtctga tgcgcccgaa cgggacagta ctgatatctg tcggcagaga
    12241 tcggctcact gaaacgacag acgtcaaaaa gatagtcatc atcacctgcc ccgcactgac
    12301 gggcccgctc cagcagtttt tccatcgata ccttcagggg agccaccaga ggaacgcccc
    12361 attccctgtg agttttactt ccctccagct taagatcaat tatgctttca tccggacaga
    12421 catccctcac acggatatgc ttcagttgat taaagcgcat tccggtataa cgcagaacat
    12481 ccagaactgt cagccagtac caggcagggt atatagcatt gcgtcgacga ctattcacca
    12541 tgttctgctc ttccgcttct gcaaacctct gcacaaccag gtaaaccctg agcatctgat
    12601 tgtcactcag tgtctttttc tttttttcag tactcgcaac tgatgcttcc accaacgggt
    12661 tttctgtctg aggcagcagt ttcatcttga tcccgaatcc cataattgcc cgcaagtggg
    12721 ccaccttgtt attccaggtc acctccctgc gcttctgctc tttcaggaca taacgccggt
    12781 atcgctgaac atcacggaca gtcacctccc ccggcaaaat atcaccgata aattccctga
    12841 ataccctgac aactttgcag tagctccact ctgtatccgg ccgaagattc tttgaaaaaa
    12901 agtactcttc cagcagttgt tcccaggtga tttttgtgac ggaatcgtca ttctgcatta
    12961 tatattcccc ttgtttactg tgctcccgca ctctccggtc aaattctgca catcatcgcc
    13021 tttactcacc cgaaaacaat aagcgggctg tctccgggaa ccgtaatatt cccgtacaga
    13081 aacctgccct taaccagata tcccgaaacc attttgtaac gtccggaatg ttctgcgtca
    13141 ctataaagac gggcctgaaa aaaacgctct cctgaacgta ccttatggat acctgcccgt
    13201 tcaaagagcg actgaacata acgacgatcc tgcccggaac cactctcctt cagaaaacga
    13261 taaaaaatcc ccggggcaca cagaaaaaca tatccggaaa caacatgaac atttgaatca
    13321 acgctgttaa ccgaaagtga accctccctc agcccctctt ttaaccatct gataaattca
    13381 ctctctaccc tgctatcctc tgtcagggaa aactctgccg ttttttccac tgtttccggg
    13441 atacggtttc cctgattttc tgttacacat ccgctctctt ccggtaactc aatacggctt
    13501 tctttatccg gcggcacgcc tgacgctatc tgccctccct catccgacaa atcagcagcc
    13561 gaaaacagcg taaggagcat tccggtatca gcatcagttt cgttcccctc atccacctcc
    13621 tgttccgctg tccggccctc attttcggca tcctccactg gcgactgtac aaccggcaac
    13681 ggctgaatga cgctttctgc tgaaattgct gctgactgca gcagtaccgg aacaacctca
    13741 gccaccggaa cttcggccac atctcctgca gcga
//
LOCUS       DAZFFI010000115        13624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFFI010000115 DAZFFI010000000
VERSION     DAZFFI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.115"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(127..879)
                     /gene="tcyN"
                     /locus_tag="ACHJ12_003984"
     CDS             complement(127..879)
                     /gene="tcyN"
                     /locus_tag="ACHJ12_003984"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4991699.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MHPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(876..1544)
                     /gene="tcyL"
                     /locus_tag="ACHJ12_003985"
     CDS             complement(876..1544)
                     /gene="tcyL"
                     /locus_tag="ACHJ12_003985"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4991700.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1559..2545)
                     /gene="dcyD"
                     /locus_tag="ACHJ12_003986"
     CDS             complement(1559..2545)
                     /gene="dcyD"
                     /locus_tag="ACHJ12_003986"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4991701.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2650..3450)
                     /gene="tcyJ"
                     /locus_tag="ACHJ12_003987"
     CDS             complement(2650..3450)
                     /gene="tcyJ"
                     /locus_tag="ACHJ12_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4991702.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3538..4089)
                     /gene="fliZ"
                     /locus_tag="ACHJ12_003988"
     CDS             complement(3538..4089)
                     /gene="fliZ"
                     /locus_tag="ACHJ12_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4991703.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4135..4854)
                     /gene="fliA"
                     /locus_tag="ACHJ12_003989"
     CDS             complement(4135..4854)
                     /gene="fliA"
                     /locus_tag="ACHJ12_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4991704.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5019..6494)
                     /locus_tag="ACHJ12_003990"
     CDS             complement(5019..6494)
                     /locus_tag="ACHJ12_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO4991705.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYQINGTDNYTVNVDSGVVQDK
                     DGKQVYVSTADGSLTTSSDTQFKIDATKLAVAAKDLAQGNKIVYEGIEFTNTGTVAID
                     AKGNGKLTANVDGKAVEFTISGSTDTSGTSATVAPTTALYKNSAGQLTATKVENKAAT
                     LSDLDLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNE
                     DAAKSLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRI
                     EDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6749..8161
                     /gene="fliD"
                     /locus_tag="ACHJ12_003991"
     CDS             6749..8161
                     /gene="fliD"
                     /locus_tag="ACHJ12_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4991706.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTQKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNELGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8176..8586
                     /gene="fliS"
                     /locus_tag="ACHJ12_003992"
     CDS             8176..8586
                     /gene="fliS"
                     /locus_tag="ACHJ12_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4991707.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8586..8951
                     /gene="fliT"
                     /locus_tag="ACHJ12_003993"
     CDS             8586..8951
                     /gene="fliT"
                     /locus_tag="ACHJ12_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4991708.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFL"
     gene            9029..10516
                     /gene="amyA"
                     /locus_tag="ACHJ12_003994"
     CDS             9029..10516
                     /gene="amyA"
                     /locus_tag="ACHJ12_003994"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4991709.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIETPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLE
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10550..10963)
                     /gene="yedD"
                     /locus_tag="ACHJ12_003995"
     CDS             complement(10550..10963)
                     /gene="yedD"
                     /locus_tag="ACHJ12_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4991710.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11150..12355
                     /gene="yedE"
                     /locus_tag="ACHJ12_003996"
     CDS             11150..12355
                     /gene="yedE"
                     /locus_tag="ACHJ12_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4991711.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12352..12585
                     /gene="yedF"
                     /locus_tag="ACHJ12_003997"
     CDS             12352..12585
                     /gene="yedF"
                     /locus_tag="ACHJ12_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4991712.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVMSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12692..13363
                     /gene="yedK"
                     /locus_tag="ACHJ12_003998"
     CDS             12692..13363
                     /gene="yedK"
                     /locus_tag="ACHJ12_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4991713.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPFDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(13403..>13624)
                     /locus_tag="ACHJ12_003999"
     CDS             complement(13403..>13624)
                     /locus_tag="ACHJ12_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4991714.1"
                     /translation="TSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3377 a         3433 c         3346 g         3468 t
ORIGIN      
        1 tacttttgca agccatcgca ttctcttatg ttattaatga gttatgctga tttgttaagc
       61 agttttatca cgcttgaaat ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt
      121 ttcttattat tgcagcagaa acttctcgag gaactggcgg gtgcgaggct gctggggatc
      181 agcaaataac gcttttgctg ccccctgctc gactatccgc ccctggtcca taaaaatcgc
      241 ccggtccgca acatcccggg caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt
      301 ttcctgcgcc agctggcgga ttgtgttcag gacttcgccc accagctctg gatccagcgc
      361 tgacgttggc tcgtcaaaca aaatcacctc aggatgcatt gccagcgcac gcgcaatcgc
      421 aacacgctgc tgttgaccgc cagacaaacg acgtggatag ctggtttctt taccggccag
      481 cccaactttc gccagcagct cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt
      541 gacgatcacc ggcccttcaa taatgttttc cagcaccgta cgatgcggaa acaaattaaa
      601 gttctggaag acaaacccga cgtgctgacg caactggcga atcagcgatt tttgctgact
      661 taatgaacgt gcagtatcaa tagtgatatc gccaacggtg atcgttcccg cttccggttg
      721 ttccagcaga tttatgctgc gtagcaacgt ggttttgccg gaaccactcg gaccgataat
      781 tgccaccact tcgccaggct ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg
      841 aaattttttc accaggttct taacttcaat ggcactcatt ttggttctct ctcctggcga
      901 ttaagttgat tctcaaaatg gttctgcaac gtcgataata ctgtcgccat gatccagtag
      961 atgagcgaag ccgccaggta catggtgaac acctccagcg tgcgcgaggt aatcaactgc
     1021 gcttgacgga acagctccgg cacctggatt gtcgcggcca gcgacgtatc ctttaccagg
     1081 ctgataaagc tgttcgacag cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg
     1141 cgacgcatag tctgccatgg cgtcatacca atactggcag cggcttccca ctgaccttta
     1201 tcaatagaag aaatggcggc gcgcagcgtt tcggcggcat aggcggcagt gttcagcgac
     1261 agaccaatca ttgctgacgg aatgggatct aattcgatac caaactgcgg caggccgtaa
     1321 tagatcataa acagttgtgc gatgagcggc gtaccacgaa atatagagat ataaaaacgc
     1381 gccagccagc gcacgggcca gattggcgac aggcgcatca gcgcgagaat aaacccgagt
     1441 agtaagccaa aaaacatgcc gccgatactg agttgcaggg tatacccagc ccctttcaac
     1501 aggaacggca aggaatcaat aaccagttgt atactttctt gcatgagcgt tcccggagct
     1561 aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc ggtatgaata aacagaatcg
     1621 gcccttcatc tttgaagcgt ttctgactga taccgtcaat cagccccgcc atcgcttttc
     1681 cggtatacac aggatcaagc agaatgcctt ccagacgcgc cagcaatttc actgcttcca
     1741 tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata gtcatcccag agtaaaattt
     1801 ccgctgatgc ggtcagctcc agttctttcg caatcgcctg ttgtaggtta accactttcg
     1861 gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat cagttcgctt tcaggcatca
     1921 ggtgttccag cccaacagcc aatccggcgt gagttccggc actgcccgat gcgaccacca
     1981 ccgacgaaat attaaccgcc ccttcacact gttgcgcgat ttccagcgca ctctccacat
     2041 aacccagcgc gcccagagca ttagaaccgc caaccggaat gacatacggg cgaaagcctt
     2101 gtgcttcgac tcgcgtcgcc agctcttcca gttgggcatt gggatcggtc agtgcgtcgc
     2161 acatttcaat ctgggtattg aacagatcca gcaacaaacg attgccgttg gttaaatagt
     2221 tttctgcggt tgtgccaata ggattttcca gcagcgccac gcagtgcaga ccgagtttcg
     2281 ccgcgactgc ggcagtctgg cgcacatggt tagactggat cgcgccggca gtaatcagcg
     2341 tatcggcacc ttcgcgcagg gcatctgccg cgagaaattc cagcttacgt aatttattgc
     2401 cgcccattgc catgggtgtg acgtcatccc gtttgatgaa aatttcccgt ccaagataat
     2461 cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc gataaactcc aaccgtggaa
     2521 aacgggttaa attatgcagt ggcataacag cctccgatgt gtgttgttgt gattttctta
     2581 ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt gaaagcgccc ttttttgtca
     2641 ttatgctgat tatttggtca catcagcacc aaaccatttt tcggaaagag cttgcagagt
     2701 gccgtctttt tgcatttccg caattgcatc attcactgct ttcagcaggt cttcatttcc
     2761 tttacgcagc gccacgccag actcctgacg ggagaatgct tcaccggtta ctgccagcgt
     2821 atcgttggtt ttcttcacca gatccagcgc cgccagacgg tcaacgagga tcgcatcgat
     2881 acggcctacg cgcagatcct gatatttggt cgggtcatca tcataggtac gcacatcgac
     2941 gccctgaaca ttctgacgca gccactcttc atagttggtg cccagaccga cacccacttt
     3001 tttgcctttc agatcagcag ccgttttaat ggtgccttcg ttaccttttt tcaccagcgc
     3061 ctgaataccg gaaatggtgt aaggggtaga gaaatcgtat tttttcttgc gctcgtcaga
     3121 aatggtgacc tgattaatca ccacatcaat gcgtttagag tccagcgacg ccagcatacc
     3181 gtcccatttg gtcggtttta gtgacgcctc aacgccaaga tgttttgcca gctgttgggc
     3241 aaattccact tcaaaaccgg ttaatttgcc gtcatctccc tgaaaactga acggcggata
     3301 agtgccttcc agccctacca gcagcgtgcc gcgctcttta actttattaa gcagaccttc
     3361 atctgcaaaa cttttaacgc tcatgcccgc gaccagcgct acggccatca cacccatcaa
     3421 tgcctgacgt cccagatgtg ctaatttcat atttaccccg aatgttgtta tgtctgtttg
     3481 cagtgtagag ccattgtttg taaacacaaa aacaactccg ctacatctta ttcttattta
     3541 atatatatca gaagcaggca ggctggagga tttctgcaca agtccggtac acgtttgacg
     3601 ctgatagtgt tgatacttcc gtaatgcgat gcggtaattg ttggtgctgg tggtgggcag
     3661 ccacggggcg aggatttcat caaggaaacc gtcctgcagc ggatcgaggg aaatattttg
     3721 ctcgtgtaga tgattcccca aacggcgcag acgaacgaca tattcacgca ccgtaccagg
     3781 gctcatttca gtttgctcaa aaagaaattg cttaaagccg ataatatcga aaaaatcact
     3841 ctgcgttttg caatgtaaat caccgcagaa tcggcacaat gccgcccatg atttttgttc
     3901 cacttgccag ccattttcat caagcagcat gtccaggcgg gaaatctcga ttttattcac
     3961 tattttgccg tcgcgaacga gggtaatgcg atcgagtaat ttacggcaat gcgcgcaatg
     4021 ggtctggctg tgtttaaagt ctttaagata gcggcttaat ggccgtcttt tcaggtgctg
     4081 caccatcatt aagaactcct ggtagtcaaa attaaagtgc ggcatttact gacgttataa
     4141 cttacccagt ttagtgcgta accgtttaat ggcctggctg tgtaactgac tgacccgcga
     4201 ttccccgacc tccagcaccg cgccaatctc tttgagattc agctcttcct ggtaatagag
     4261 ggttaatacc agtttttcgc gctccggcaa cgtttcgatg gcttccatca cccgctggcg
     4321 cagattactg tccagcagtt gttgtagcgg attttctcgc tgatgatcat cagtaaccag
     4381 ttcgatgcta tcgccgtgct cttcgcgcca ctcatcgtag gagaagagct ggctgttatt
     4441 ggtgtcgagc aacatttggc gataatcggc aatatcgatc cctaaacgtt ccgctacctc
     4501 agtttccgtg gcgttgcggc caagttcctg ctccagttgc cctattgcct gtgccacttc
     4561 acgcgcgttg cgtcgcacgc tgcgcggcac ccagtcacgg ctgcgaagtt catccagcat
     4621 agcgccacgg atacgctgca ctgcgtaagt tgtaaatgcc gttccttgta gggcgtcata
     4681 gcgttcgacg gcattaagta acccaatgcc gcccgcctgt agcagatcgt caagttccac
     4741 gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga cgcaccagcg ggacataacg
     4801 ctgccacagc gagtgtttat ccattacacc ttcagcggta tagagtgaat tcacgataaa
     4861 cagccctgcg ttatatgagt tatcggcatg attatccgtt tctgcagggt ttttaatcgg
     4921 acgattagtg ggtgaaatga ggggttattt gggggttacc ggtaaattgc ggacagaaaa
     4981 aaccccgccg gtggcggggt ttgagcgata agtgtaaatt aacgcagcag agacagtacg
     5041 ttctgcgtgg tctggttagc ctgtgccaga acagaggtac ccgcttgttg caggatctgc
     5101 gcacgagaca tgttagacac ttcggtcgcg tagtcagcat cttcgatacg gctacgggca
     5161 gaagacaggt tgtttacggt gttgccaagg ttggtgatgg cagagtcgaa acggttttgt
     5221 actgcaccga ggtcagaacg cagattgtca actttagcca atgctttgtc gatagtttcg
     5281 agcgggttgg tggtagattg caacgatttt gctgcatctt cgtttaccag aatcgggcta
     5341 ccaccttctg atttgctcag atacatgact ttattgttac cagaagcagt ctccgttatc
     5401 gttttaccat ctgcactaac atcgtaagtt gcaccgttaa caactaacgt gcttcctgtt
     5461 ttcttggcag cgttcagatc aagatcagat agtgtcgctg ctttattttc aacttttgtt
     5521 gcagtcaatt gccctgcact atttttgtat agggctgtcg taggggcaac ggttgcacta
     5581 gtacctgatg tatcagtact ccccgaaata gtgaattcaa cagccttacc atcaacattg
     5641 gcggttaatt taccattacc tttggcatct atagcgacag tgccggtatt tgtaaattcg
     5701 ataccttcgt agacaatctt attcccttga gctaaatctt tagcagccac tgcaagctta
     5761 gttgcatcaa tcttgaattg agtatcactg ctggtcgtaa gtgaaccatc cgcagtactc
     5821 acataaactt gtttgccatc tttatcctgt actacgccac tatctacatt aacagtatag
     5881 ttatcagtac cgttaatttg ataattatca gtacctgtcg ctttaaattt agaaatcagg
     5941 tcactgccgg ttgctttctg cgcgccatcg atattaaaac cgtccaggcc gagagttttc
     6001 gcatcaattt ttgccagatt gatagtgatg gtttcaccat cattagcacc aacctgaatt
     6061 ttcatttcat tattttcagc aaggactttc acgccgttaa actgagtttg ctcagataca
     6121 cggtcaattt cttccagacg ttgagtaatt tcagcctgga tagaagaaag atcgctgtca
     6181 gagttagtac cgttagttgc ctgaacagaa agttcacgaa tacgctgcag gttgttgtta
     6241 atttcattca gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg
     6301 gaagcctgag tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct
     6361 gcgtcatctt tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc
     6421 agagaagact gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta
     6481 atgacttgtg ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc
     6541 cacggtttct caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta
     6601 gtttctctcc tgttttttct taacggctga aatatccgta atatacaact ctactctgcg
     6661 catcatcttc gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc
     6721 tactgtttgc aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc
     6781 gaatctgccg ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa
     6841 tccaattact cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa
     6901 aagtgcatta gaaaaattcc agactgcaaa tactgcgtta aataaagccg atctgttcaa
     6961 aagcactacc gttaccagta gcaatgaaga ccttaaagtt tccaccgaag caggtgctgc
     7021 ccccggtatc tataccatca gcgtaaccca actggcgcag gcgcaatctt tacgaactga
     7081 ttccccgaca atcattgcat caacaaaaga tgcactgggt gatgagtcat cagatacacg
     7141 cacaataaaa attacccaag acggccgtaa agagccgctg gaaatcaagc tcaataaaga
     7201 tcagacgtca ttggatgaga tcagcaaagc cattaacgat gccgatagtg gaatttccgc
     7261 tagtatcgtt aaagtcaaag atggtgacta tcaattggta ttgaccgcca gtgaaggtct
     7321 ggccaataaa atgaccatat ctgttgaggg tgacagtaaa ttaaatgatc tgctggctta
     7381 tgacagcaag accaacaccg ggaatatgaa ggaactggtt aacgcgcaaa atgcacagct
     7441 aaacgttaac ggtatcgata ttgaacgttc aagtaacaaa attaccgatg ctcctcaggg
     7501 agttactctc gatttgaccc aaaaagtcac tgacgtccgg gtcacggtga caaaaagcaa
     7561 cgataaagca accgaagcaa ttaaaggctg ggtggattca tataactccc tcattgacac
     7621 ctttaacacc ctgacaaaat ataaagaagt agacccaggt gctgaagcac aagataaaaa
     7681 taatggtgca cttctcggag acagcgttgt acgtacaatt caaagcggca tccgcgcaca
     7741 atttgcaaac ggtgccagcg acggtgcctt caaaacatta aacgagctcg ggattaagca
     7801 ggacggcacg acgggtaagc tgaaaattga tgatgataaa ctcaaaaaag tcctgaatga
     7861 aaatacagca tccgtacgtg aattgctggt aggcgatggt aaagaaacag gaattaccac
     7921 caaaattgcc accgaggtaa aaggttacct tgccgatgat ggcattattg atagcgcaca
     7981 ggacagtatt aacgccacac tgaaaaaact gaccaaacag tatctatccg tcagtgccag
     8041 cattgatgac accgtggcgc gttacactgc acagtttacg caattagata ccatgatgag
     8101 caaactgaat aacaccagta cctatctcag ccaacaattt actgcaatga gtaattcctg
     8161 atcacggagg tcagcatgta taccgcgagc ggctcaaaag cctatgcaca aattggcgtc
     8221 gaaagcgccg taatgagcgc cagccagcag cagctggtca ccatgctatt tgatggagtg
     8281 ctgagcgcac tggttagagc gcgcctgttt atgcaggaca acaatcagca aggcaaaggc
     8341 gtctctttgt caaaagcgat caacatcatt gagaacggac tgcgggtgag tcttgatgaa
     8401 gagagcaaag acgaattaac ccaaaacttg attgctcttt atagctatat ggtcaggcgc
     8461 ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg aagaagtgga agcattaatg
     8521 cgcaatattg ccgatgcctg gaaagagtct ttactctccc cttctttgat tcaggaccca
     8581 gtctgatgaa ccatgcaccg catttgtatt tcgcctggca acaactcgtc gaaaaaagcc
     8641 agctcatgtt acgcctggca acggaagaac aatgggacga actcatcgcc agcgaaatgg
     8701 cgtatgtgaa tgcggtgcag gagattgcac atctgactga agagattaac ccatccacca
     8761 cgatgcagga gcagctccgc ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa
     8821 agcagttatt acagattcgg atggatgaac tggcgaaact ggtcggtcag tcatcggtgc
     8881 aaaaatcggt gttaagtgcc tatggcgatc agggcggctt tgtgctggct ccgcaagata
     8941 acttcttgtg aatcttaaca agctaatggc tcgcgaataa tctgattacg gctacgcttc
     9001 taatgttccc cttgaatgga gtcgaagaat gcgtaatccc acgctgttac aatgttttca
     9061 ctggtattac ccggaaggcg gtaagctctg gcctgaactg gccgagcgcg ccgacggttt
     9121 taatgatatt ggtatcaata tggtctggtt gccgcccgcc tataaaggcg catcgggcgg
     9181 gtattcggtc ggctacgact cctatgattt atttgattta ggcgagtttg atcagaaagg
     9241 cagcatccct actaaatatg gcgataaagc acaactgctg gccgccattg acgccctgaa
     9301 acgtaatgac attgcggtgc tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga
     9361 aaaagaagct attcgcgtgc agcgtgtaaa tgctgatgac cgtacgcaaa ttgacgaaga
     9421 aatcattgag tgtgaaggct ggacgcgtta caccttcccc gcgcgtgccg ggcaatactc
     9481 gaagtttatc tgggatttca aatgttttag cggtatcgac catatcgaaa cccctgatga
     9541 agatggcata tttaaaattg ttaacgacta caccggcgaa ggctggaacg atcaggttga
     9601 tgatgaatta ggtaatttcg attatctgat gggcgagaat atcgattttc gcaatcatgc
     9661 cgtgacggaa gagattaaat actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg
     9721 ttttcgtctt gatgcggtca aacatattcc ggcgtggttt tataaagagt ggatcgaaca
     9781 cgtacaggaa gttgcgccaa agccgctgtt tattgtggcg gagtactggt cgcatgaagt
     9841 cgataaactg caaacgtata ttgatcaggt ggaaggcaaa accatgctgt tcgatgcgcc
     9901 gctgcagatg aaattccatg aagcatcgcg catggggcgt gactacgaca tgacgcagat
     9961 tttcacgggt acattagtgg aagccgatcc tttccacgcc gtgacgctcg ttgccaatca
    10021 cgacacccaa ccgttgcaag ccctcgaagc gccggtcgaa ccgtggttta aaccgctggc
    10081 atatgcctta attttgttgc gggaaaatgg cgttccttcg gtattctatc cggacctcta
    10141 cggtgcgcat tacgaagatg tcggcggtga cgggcaaacc tacccgatag atatgccaat
    10201 aatcgaacag cttgatgagt taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac
    10261 attatttttc gaccatccga actgcattgc ctttagccgc agtggcactg acgaatatcc
    10321 cggctgcgtg gtggtcatgt cgaacgggga tgatggcgaa aaaaccatta atctggaaga
    10381 gaattacggc aataaaacct ggcgtgattt tctggggaac cggcaagaga gcgtagtaac
    10441 cgacgaaaac ggtgaagcga ccttcttttg caacggcggc agcgtcagcg tgtgggttat
    10501 cgaagaggtg atttaaattc atccccggcg gcaagccggg gagatttcat tacggcagtg
    10561 gcgtcggtaa cggcgcttta tccagcgcgg cggcacactc tgccgtcggg cgatccacac
    10621 gctccatcgt cataccatcg tactcaatgg tgttaccgtc acgctcaacc tcgtacagct
    10681 cacgttttac ggtaacgtta gtgagatcat cagacatcaa tgtcaacttg cccggcacgg
    10741 caatcacccg ctgccactga cggcaatcca gggtatcgcc ctctttagtg acaatcaggc
    10801 tgcctatcgc ttccggactg accagcgcac gttgcggccc tttggtctgc cagtagcccg
    10861 ccagccagtc cggcgcgggg gttttcacga cattgttata gttttcgact tcggcacagc
    10921 ccgccagcaa catgagcgcg cctgcaattg cgagtttttt catcattcgt cctgcatgag
    10981 aagaaaagag gattgtggca ttaaagccct gatgtcgcca gcctttccag gttcagagag
    11041 aaatttgatc taactattat ttttgtgaat tattaccccg aaaataaatt tgcatggtgt
    11101 atgattcgcg actcttgttt caaaccttct gagttcagag gctacattca tgtcatggca
    11161 gcaattcaaa cacgcctggt tgattaaatt ctgggcgccc atccccgcgg tcatcgcggc
    11221 gggtattctc tccacttact attttggcat tactggcacc ttttgggctg tcacgggtga
    11281 atttacccgt tggggcggcc agctcctgca actgttcggc gtccatgctg aagagtgggg
    11341 ttattttaaa attatccatc tggaaggatc gccattaacc cgcatcgacg ggatgatgat
    11401 cctcggtatg tttggcggct gctttgccgc agcgctgtgg gccaacaatg tcaaactgcg
    11461 catgccgcgc agccgtatcc gcattatgca ggccatcatt ggcggcatta tcgccggttt
    11521 tggcgcgcgt ctggcaatgg gctgtaacct ggcggcgttc tttaccggta ttcctcagtt
    11581 ctcgctgcat gcctggttct ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg
    11641 ctttaccctg ctgcccatct tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc
    11701 accgctgacg caaaaaccgg atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt
    11761 tttcggcatg ctgggctggg cgctgctcac ggcgatgaac caacccaaac tggggctggc
    11821 aatgctgttt ggcgtcggct ttggtttact gattgaacgt gcgcaaatct gctttacttc
    11881 agcgttccgc gatatgtgga tcaccggacg tacccatatg gcgaaagcaa tcattattgg
    11941 tatggcggtg agtgccatcg ggatcttcag ttacgtacag ttaggcgttg aacccaaaat
    12001 catgtgggcg ggaccaaacg cggtaattgg tggtttactg tttggttttg gcatcgtgct
    12061 ggcaggcggc tgcgaaaccg gctggatgta ccgcgcggta gaaggccagg tgcactactg
    12121 gtgggtcggt ctgggcaacg tgatcggctc aacgattctg gcgtattact gggatgattt
    12181 cgctccggcg ctggccaccg actgggacaa aatcaacctg ctgaaaacct ttggcccgat
    12241 gggtggcctg ctggtgacat atttgctgtt gtttactgcg ctgatgttga ttatcggctg
    12301 ggaaaaacgc ttcttccgcc gtgcggcacc gcagactgct aaggagatcg catgaaaaat
    12361 atcgttcctg attaccgcct tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca
    12421 actcttgagg cgatgccgca gttaaaaaaa ggggaaatcc tggaagtgat gagcgactgt
    12481 ccacagtcga tcaataatat tccactggat gcgcgtaatc acggctatac ggtgctggat
    12541 attcagcaag acgggccgac cattcgttat ttaattcaga agtaatccct cattcccgct
    12601 gtggcccaca gcgggaacat cgttgcctct gacacataga ttttacccgc cccctctcct
    12661 attcttttca gtatgatctc aggaggtttc tatgtgtgga cgctttgccc aatcccaaac
    12721 gcgtgaagat taccttgcgc ttctcgcgga agatattgaa cgcgatattc ccttcgatcc
    12781 cgaacccatt ggcagataca acgtcgcgcc gggaaccaaa gttcttctgc tcagtgaacg
    12841 tgatgaacac cttcatctgg atccggtttt ctggggatat gctcccggat ggtgggataa
    12901 accgccgctg attaacgccc gcgtagaaac tgcggccacc agtcgtatgt ttaaaccgct
    12961 ctggcaacat ggtcgggcaa tctgttttgc cgatggctgg tttgagtgga aaaaagaagg
    13021 cgacaaaaaa cagccttatt ttatctatcg cgctgatgga caacctgttt ttatggccgc
    13081 gataggtagc acaccgtttg agcgtggtga cgaagccgaa ggatttttaa ttgtcactgc
    13141 tgcagcagac cagggtctgg tagatattca tgaccgccgc ccgctggttc tgtcgccaga
    13201 agctgcacag gaatggatgc ggcaagagat tggcggtaaa gaagcatcag aaattgctac
    13261 caacggctgt gtgccagcaa accagttcac ctggcacccg gtatcgcgcg cggtaggtaa
    13321 tgtcaaaaac cagggcgcgg agttaattca acctgtttgt tgacatcctc cacgccctga
    13381 aggacgagga tccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgggcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcgg ttctctttcg ctgtatgacc acagtacgcg caacgctggc
    13621 ttgt
//
LOCUS       DAZFFI010000116        13619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFFI010000116 DAZFFI010000000
VERSION     DAZFFI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.116"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..596)
                     /gene="yeeO"
                     /locus_tag="ACHJ12_004000"
     CDS             complement(<1..596)
                     /gene="yeeO"
                     /locus_tag="ACHJ12_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4991715.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTALSYPAAAITLIGSGALRGAGNTKIPLLING"
     gene            757..832
                     /locus_tag="ACHJ12_004001"
     tRNA            757..832
                     /locus_tag="ACHJ12_004001"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:790..792,aa:Asn,seq:gtt)
     gene            complement(870..1820)
                     /gene="cbl"
                     /locus_tag="ACHJ12_004002"
     CDS             complement(870..1820)
                     /gene="cbl"
                     /locus_tag="ACHJ12_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO4991716.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNSEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1922..2839)
                     /gene="nac"
                     /locus_tag="ACHJ12_004003"
     CDS             complement(1922..2839)
                     /gene="nac"
                     /locus_tag="ACHJ12_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4991717.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3166..3241
                     /locus_tag="ACHJ12_004004"
     tRNA            3166..3241
                     /locus_tag="ACHJ12_004004"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3199..3201,aa:Asn,seq:gtt)
     gene            complement(3297..4232)
                     /gene="ldtA"
                     /locus_tag="ACHJ12_004005"
     CDS             complement(3297..4232)
                     /gene="ldtA"
                     /locus_tag="ACHJ12_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4991718.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4294..5373)
                     /gene="cobT"
                     /locus_tag="ACHJ12_004006"
     CDS             complement(4294..5373)
                     /gene="cobT"
                     /locus_tag="ACHJ12_004006"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4991719.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5385..6128)
                     /gene="cobS"
                     /locus_tag="ACHJ12_004007"
     CDS             complement(5385..6128)
                     /gene="cobS"
                     /locus_tag="ACHJ12_004007"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4991720.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6125..6670)
                     /gene="cobU"
                     /locus_tag="ACHJ12_004008"
     CDS             complement(6125..6670)
                     /gene="cobU"
                     /locus_tag="ACHJ12_004008"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4991721.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            6837..7028
                     /locus_tag="ACHJ12_004009"
                     /pseudo
     CDS             6837..7028
                     /locus_tag="ACHJ12_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(7129..7458)
                     /gene="tmaR"
                     /locus_tag="ACHJ12_004010"
     CDS             complement(7129..7458)
                     /gene="tmaR"
                     /locus_tag="ACHJ12_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4991722.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7630..8689)
                     /gene="yeeA"
                     /locus_tag="ACHJ12_004011"
                     /pseudo
     CDS             complement(7630..8689)
                     /gene="yeeA"
                     /locus_tag="ACHJ12_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(8887..9360)
                     /gene="sbmC"
                     /locus_tag="ACHJ12_004012"
     CDS             complement(8887..9360)
                     /gene="sbmC"
                     /locus_tag="ACHJ12_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4991723.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(9479..10645)
                     /gene="dacD"
                     /locus_tag="ACHJ12_004013"
     CDS             complement(9479..10645)
                     /gene="dacD"
                     /locus_tag="ACHJ12_004013"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4991724.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            10854..12281
                     /gene="sbcB"
                     /locus_tag="ACHJ12_004014"
     CDS             10854..12281
                     /gene="sbcB"
                     /locus_tag="ACHJ12_004014"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4991725.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(12324..12551)
                     /gene="tsuB"
                     /locus_tag="ACHJ12_004015"
     CDS             complement(12324..12551)
                     /gene="tsuB"
                     /locus_tag="ACHJ12_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO4991726.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(12565..>13619)
                     /gene="tsuA"
                     /locus_tag="ACHJ12_004016"
     CDS             complement(12565..>13619)
                     /gene="tsuA"
                     /locus_tag="ACHJ12_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO4991727.1"
                     /translation="SMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYALL
                     IAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGE
                     GLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFHLSVWPLVAVLLV
                     ITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEA
                     TGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAAD
                     AQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLV
                     YVRPQRKARLATAAAN"
BASE COUNT         3651 a         3621 c         3061 g         3286 t
ORIGIN      
        1 ctaccgttaa tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca
       61 atcaaagtga tggcagctgc tggataactg agtgccgtca gctccagata agtcaacgcc
      121 agtgctttaa cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa
      181 tgatgaataa gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc
      241 gtcgccaccc tcgctcgtcg tcgatcccgc ttaccgagac taaatgccac aacaacagta
      301 gtaccaagat cgatagcagc aaaaaaagcc ataatgacca tattgaagct gtccgccaat
      361 cccacgccgg ccatcgcatc ttttcccagc cagctgacca gaaaagtgct caggacaccc
      421 atcaacagga cacaggcatt ctccatgaag ataggaacag caagcggggt tatctcgcgc
      481 cagaacaaca ctttgtagct cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg
      541 cgtaaagcag aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca
      601 aataatgagg gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg
      661 caacaaactg ttgatagaaa cggcaaacag ttggggaatt taaaaatcgg gtttgacaaa
      721 agatttttcg ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa
      781 cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcctg
      841 aaaagcccgc ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg
      901 ttttccatca cctgccgctt gatatcctca actgacagtc ctgcattaca aagttccaga
      961 aagcgccaga catagttacg ctgaagttgt cctcgcttca gtcccaacca gacagtatta
     1021 gcatcgaaaa gatgccgcgt atccagacgg attaaattct cttcctcttg ttcgccgctg
     1081 gattgttcgg caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca
     1141 gaatcctgcg cacttaatac aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca
     1201 atacgtgagc gccccgtaat cccctgtcgg taagtgatta acggccactt cgctattgat
     1261 tccagcgtca atggtgaaat ttgcgttaag ggatgatcga gtggaacaag caaactatgg
     1321 tgccaacgaa accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg
     1381 ataccaatat cagcttcgct attttgcaac aacgtcgcaa tttcctgtgg cgtcccctgg
     1441 attagctcga gccgtacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag
     1501 ctataacgcg cctgagtatg cgtcgttgca atagtgagaa cgccagacgt atcgttggta
     1561 aacaggtctg caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc
     1621 agtaatgctt tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt
     1681 tcgataccaa gttcatcctc cagttcccga atatgacggc tgacgccgga ctgtgaggta
     1741 aaaagcatat tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc
     1801 tttagttgtt ggaaattcac ggtaaactcc gggcagttca gatttcccgt tattgttaaa
     1861 gtctaatgcc cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg
     1921 cttagctcac caattgccac tgcctttttt ccatcactgg agaactaatc actgacatta
     1981 acaactcttt cactgcctgt gcctgtggtg ataagttcgc tctggcgggt aaatttaatg
     2041 acaaagagag actcatggaa ggagtggtaa tgcgtgacat ccacccattt actgcgccac
     2101 ataacgaacg cgcggccgat tcgggtaata ctgcaacgcc catgccgctg gcaatcgctg
     2161 cggtaagcgt ggcaatagac tcaatttcac caataacttt tgccgtgagt cgccgtaggg
     2221 aaaaagcctc atcaacacga agtctaatag cactgtaatc actggggaga aagaggttca
     2281 ttttcgcaat agcattcaca tcaacgcttt gccccgggca atcttgagtt cctaccagaa
     2341 aaagatcttc tttcagcaaa gcctgactgg atacaccagc cacaggggaa tgctcataaa
     2401 tcaccgccat atcgagttgg tgatttatca atttttcgtt aagcactgca ccactatttt
     2461 catgaagata gataacgatc tccggaaatt cagcgcgaac cgcctgtaat aagggcatgg
     2521 tgatggatga cgcagcggtt cctggtgcaa agccaatcga gacttgcccc gataatgcct
     2581 gaccaacgtt atgcaccgcc agttgggcct gttcacactg acgtaaaatg gcccgcgcat
     2641 gggtatagag aatttttccg gcgtctgttg gcgtaacgcc ccgctttgta cggatcaaaa
     2701 gttgttgatt taactcacct tccagtgtgg caacctgctg gctgagcgct ggttgtgcga
     2761 tatgcaatac ttcagcagcc tgggtcaggc taccaatatc tacaattttt acgaagtatt
     2821 tcaggcgtct gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt
     2881 acttacaacc agattgcaag atgcttgcca gttttatttt ggcgttgatg tacaagctaa
     2941 ccaactgtca aataagagat tatgatagat tcgtcatttg ctcctttaat cagctgtcgc
     3001 gttcccctgc cctataaaag gagggtatgc accacgatga ttcattaccc aataagattg
     3061 aaagctcacc actttgttga aattgacagc aaacaaacaa aaaaatgcat ttcacccttt
     3121 gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc tgtagttcag
     3181 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     3241 aaattcaaaa aagcctgctt tctagcaggc tttttgcttt ctaattacca acgctcttaa
     3301 aacatctgtc ttgaaccaga actaatttgc acaggcattc ccgatcgacg ttgcaacgca
     3361 gcatttgcgc gatttacatc aacttcttgc ccgctgataa acgcccgcaa agatggggtt
     3421 accggcaatg gcacttttcg gtcagactca tattctgcac gattgcgcga caacggctca
     3481 tgaacttcca gccagttcga gccatctggt tcagtggtgt attttactgg ctggtcgata
     3541 atttgcacac gcgtcccaac aggaacatta tcaaacagat atttgatatc gtcattgcgc
     3601 agacgaatac agccctgact tacccggagc ccaataccaa aattggcatt ggtaccatgg
     3661 atggcataca acctgccaat ataaatcgcg tacagcccca tgggattatc ggggcccgca
     3721 ggaacaaatg cgggcaaact ctcccctcgt ttcgcatatt cgcgccgagt gttcggcgtt
     3781 ggcgtccagg ttggagcttc ttgtttacgt tcaacggtag tcacccagtt acgcggggtt
     3841 tctcgcccag cctggccgat accaatagga aagacttcca cagtattact gtctggtggg
     3901 tagtaataaa gacgcatctc agcgacgtta accacaatcc ctttacgaac agtggcgggc
     3961 aaaatcagtt gctgcggaat ggtgagttgc gagccagact tcggcaaaaa aacatcagcg
     4021 cccgggttcg cttccagcat gttacttaac ccttgcccgt attgtgcggc aaaagtctcc
     4081 agcggctggg tattgtgatc aggaacagtt acagtaaacg actgccccac taaacggcta
     4141 ccctctggag gtaatggata agttaccgcc aggctagtat ggctggcaaa aagtagagca
     4201 aatgagcaaa aaatatttac acgacgcatc atgtcccttt cctaagtcgc gaaagctatc
     4261 cgttaagtat agcttttatc agacttttcg tttttaactg ttcaaatcag aagtcgtatt
     4321 ccccggtaga acaatattac tggcagcaag ttcgcccatg ttgttatata tcgcacaggc
     4381 agcttcgatg atgggcatcg ccagagctgc gccactcccc tcacctaaac gcatctccat
     4441 attgagataa ggctccagcc ccaaatgcga cagcgcaata cgcgcccctt tttctgccga
     4501 caggtgagaa ggaatgagat acggtttgat tgcaggagac atctggcagg ctgcgagcgc
     4561 ggcagcataa gaaagaaatc catccagcaa cacgggtaaa ccgcaggaag cagcgcctaa
     4621 catcacgcca gccattccga ccaaatcaaa tccacccact tttgccagga catcaacgcc
     4681 atcctgcgga tttggttgat tcaacgtaat cgcccggcgc acaacatcaa ttttattagc
     4741 cagtttatct gtcggcaggt ttgcgccaat cccaaccact tcttcaggag cccggccagt
     4801 gattgtgctg actattgccg ctgccggtgt cgtgtttgcc atccccagtt cacctacacc
     4861 aaacagcgta acaccgtttt ttgccagctc ccgcgtataa catatgacgt ccaaaagcaa
     4921 cttttctgcc tgatggcgac tcattgccgg agctgaagca atattgccac tacctcgagc
     4981 aacacgcatg ttgataagcc cggggatagg ctcagcagta tcaataccaa catcaattac
     5041 gtagacgtta gcgcccgctt gtgctgccag cacacacacg ccggttgttc cgcgggtcat
     5101 attttcagcc tgaatggctg tcacttcttt tggggaaata gcgacccctt cctcccagac
     5161 gccgtgatcg gcacacataa ccagtaccgc ttttttgccc acatgcggaa cgccattcaa
     5221 ccccggcatt cctgccagtt gtatggcaag cgcctccagc cttcccaggc taccaacagg
     5281 tttgagtaac ccgtcaatat gccgttgtgc acgcgacata gcggcaggat cgatagcagg
     5341 gatcgtattc agtaaatcgg caagtgtttg catgtcacgt ctctttatag cagtgccagc
     5401 aggaagacca gttcaccaag ctctatagca gctcccagcg tatcgccggt ttgcccgccc
     5461 agcgtacgtt tgagaagctg accgaggatg aaaatcgcca ccatcgtcac caccatagca
     5521 gccacaccat gcatacccgg caataatacg gcagcaaaaa tagcggctaa gccgagtgtg
     5581 acgcaggttt gtcgcccatc aattttgcca atgaacacat tgccaagtcc ttcttcccgg
     5641 gcataacgat gacgatacat caataatgcg gcagtcccgc ggctgaccgc acatgctgcc
     5701 gccagcgatg caaggatcgg ttcgccacgc agagccaact cgcttaatac cagaatcttt
     5761 gccagtacaa caaaaatcaa tgccagaccg ccgtgggtgc ctaaacggct atcacgcatg
     5821 atttccaaca tgcgatcgcg gctacgtgca gaaaatacgc catcacaggt atcggcgagg
     5881 ccgtccaggt ggaatccccc ggtcatcagc gccagcacaa gtacgctaaa cagtgccgcc
     5941 agtggtacgc cacaccatgc ctgcagcacc atgaagacca gcccgctaat cgcgccaagc
     6001 aataatccaa ttaaaggaaa agtaataata ccgcgagaat aatgctcgaa atccagtccc
     6061 tgggaccagc gacgcggtac aggcagacgc gtaataaaag agagcatcgc ccaaaataat
     6121 ttactcattt gatttttact ccaatacccg aaaccaccaa ccatacttca tttgccgcag
     6181 cggccagctg ctgatttacc cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct
     6241 ccggcacaat ccccattccc acttcgttag tcactaatac aacctttgcg ggacaacgtt
     6301 ggcaggcagc aatcaacgac tgaatctcag cattaatcgc ctgttccatc gcctgataat
     6361 cccattcatc agggtcttta tcgccgccat aatcaaacaa cagattagtc accatggtgg
     6421 taacgcattc cagcaataca gcctcatgcg ggttaatgtc tgcatgaatt aattcatcaa
     6481 gatgttgcca acgctccacc gtgcgccagt gcgccgggcg gccttgccga tgatgttcta
     6541 tccgtgcagc catctcatca tcaaggattt gtgaggtggc gatatacaga acctgtgaag
     6601 agtccccaat aagcgcctct gcatggcgac tcttcccgct ccgtgcgccc cccgtcacca
     6661 aaatcatcat accgactcct gattcaattg tatgatggta ttcattgaac agaacaatgg
     6721 atcccccacc gttgctgacc gcaaaacatt tacgcgatta atactgcgcg taatataatt
     6781 aaaaatccag cattctcaat ccattacgaa agaagatggc attgtggaat agccagactt
     6841 cctcgtgtta acgcccggtc attggagtag taaccaaagc agatttagcc aacatggaac
     6901 aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg ttagtaacca
     6961 gtgcggttaa taacaataga gttgcagaac tttttgcctt gctgcatata gaagaagtct
     7021 gtcgctaatt tcgacaggtt atcagatggg aatgatgact ttgatacaca gaaaagaatc
     7081 aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt acttcgcttc
     7141 gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc gttctttgga
     7201 gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct
     7261 ggcgatgatg ccctggattg cttcaacgct catccccggc ttgatgtaat cgctcaggtt
     7321 gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt cctgaatttc
     7381 acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt cctggaatga
     7441 aggcttggta gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca
     7501 accgctattc gttagggcca acattactca tagctgccgc agatgacaat gcttttatcc
     7561 cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgaa
     7621 gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact caagctgatg
     7681 cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta ccttcaggtc
     7741 atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt tttccgtatt
     7801 ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg
     7861 ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg
     7921 gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat
     7981 ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg cgggcgcaat
     8041 cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg
     8101 gcgttcgagt aagttcggtg agaatgcaga ttgatagata ccgattatac tcggtcagac
     8161 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     8221 acattgccag taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt
     8281 cacctgtcgg ggaaccaaca acaattgcca gcgtcacccc aatcaataat ccttgatacg
     8341 gtttcttgcc cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca
     8401 tcagcggtaa cgaaattaac tccagttgca gagcgataag acctaaaatc gaacccaaca
     8461 ccgtaccgcc aatacgttcg aaggcgcgag ggacaacgtt accccagaac gagattgacc
     8521 ccataatcac caccatggtg accagcggcc aggtgctttc cgggatagta aacaggcgga
     8581 taatgagaaa agtgagcagg aatgccagcg cgacccgagt accatgcaca atgcggtaat
     8641 ggcggtatac ccggatttca aacgggctta atgacttatc ggcacgcacg cagaaactcc
     8701 gtaatgaaga ttaaaagcgc tcataactca tctgtcggcc agaaggcgca cctctgagtt
     8761 atccgacgga cataccggga acagtgtaat ttcccggcag gagcgaaaaa tttgaaaggc
     8821 gcatcttact cttttcgctt tcaaaaaaag agtggtcttc gcgtcaacac accgccctga
     8881 gatgaattag tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc
     8941 ttccgcgcca ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact
     9001 gtcctgcaag agactattaa agaactgata ccagggttta gcaaaatcat caccgactac
     9061 acgagctacc gccaccgcat actgaccacc tgtaatttct gtcagaatga cgccctcact
     9121 gttttcggga agcgtaaagt aacccggcac cgtcacgacg gtgtcgcagc gtaatttttc
     9181 ggcgggtgtt tcatctggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt
     9241 tttgttatct acccacatca tcaactgctc aaagcctttc tttaccgtct gttcccacgg
     9301 gccaacgaga tggaaacctg caacggtacg tttctcttcc tgcttaatct cgtagttcat
     9361 gacgcctcca ttaatactgt ttttatatac agtatagttg caatattaaa accacaagga
     9421 atgagtgttg attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc
     9481 aggccttatg gtggaaataa tcactcaggc gggaaaacat gccgccttcc ccgacagatt
     9541 ccagggtaac cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc
     9601 ctacccgctg atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg
     9661 ctttgatatg tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca
     9721 ggtcgatatt ttctttatcg ccataccaga tgcgttccgt tccgaccttt ttcccacggt
     9781 gcaaaatttg caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt
     9841 cctcacgacc ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat
     9901 ctacagccga agcaatgagg ttaaaaccgg caccagaagt atgacccgtt ttcaggccgt
     9961 caacattcat ggttttatcc cacaacaacc cgttacggtt ttgctgggtg ataccgttcc
    10021 aggtgagact tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc
    10081 gagaaagcac agctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca
    10141 ctgtttcaaa atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa
    10201 caaactgccg ttgcccaccg gcaatatagt cagccagagc aacacaagcg tcatttccgg
    10261 aatccacaat taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa
    10321 acatcagtga agaaccgaca aacaccggat tatctttcgc ccacgcatcg cgccccacgg
    10381 tgacaatatc gtctggcgta atgcgatgac tatcgatagc gcgatccacg acataacccg
    10441 tcatcagctt tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagga
    10501 tctgaccggt ggtgtaatcc atcagtaccc aggacccggc atgaatctgt ggaggctgag
    10561 gtgaaaaagg aatgttttcc gccgcaaaac cagacgataa gttaaaaacg aacaaagaag
    10621 cagcaataat aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt
    10681 tttacggaaa tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctactctctc
    10741 atttttatct gacatgatct gttgccactc gctgtcaaat tgttgcgcta aagctgatta
    10801 gcacggtgat atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga
    10861 atgacggtaa gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggcacgcacc
    10921 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatagcgaa ttcaatgtca
    10981 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag
    11041 ccgtattaat taccggtatt accccgcagg aagcacgggc gaaaggagaa aacgaagccg
    11101 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
    11161 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
    11221 cttacgcctg gagctggcag catgataact cgcgctggga tttgctggat gttatgcgtg
    11281 cctgttatgc cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga
    11341 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
    11401 cgatggctga tgtgtacgcc actattgcga tggcaaagct ggtaaaaacg cgtcagccac
    11461 gcctgtttga ttatctcttt acccatcgta ataaacacaa actgatggcg ttgatcgatg
    11521 ttccgcagat gaaacccctg gtgcacgttt ccggaatgtt tggagcatgg cgcggcaata
    11581 ccagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggtgg
    11641 atttggcagg agacatttcg ccattactgg aactggatag cgacacattg cgcgagcgtt
    11701 tatataccgc aaaaaccgat cttggcgata acgccgccgt tccggttaag ctggtgcata
    11761 tcaataaatg tccggtgctg gcccaggcga atacgctacg cccggaagat gccgaccgac
    11821 tgggaattaa tcgtcagcat tgcctcgata acctgaaaat tctgcgtgaa aatccgcaag
    11881 tgcgcgaaaa agtggtggcg atattcgcgg aagccgaacc gtttacgcct tcagataacg
    11941 tggatgcaca gctttataac ggctttttca gtgacgccga tcgtgcagca atgaaaattg
    12001 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtc gataaacgga
    12061 ttgaaaagct gttgttcaat tatcgggcgc gcaacttccc ggggacgctg gattatgccg
    12121 agcagcaacg ctggctggag caccgccgcc aggtcttcac gccagagttt ttacagggtt
    12181 atgcagatga attgcagatg ctggcacaac aatatgccga tgacaaagag aaagtggcgc
    12241 tgttaaaagc gctttggcag tacgcggaag agatcgtcta atttgaagcc tccgccgctg
    12301 gtacggcgga ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
    12361 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
    12421 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
    12481 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
    12541 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg
    12601 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
    12661 gccaatccag ccctgccagg tcatcattgc agtcataacc agtccattac cgatagagca
    12721 accacctgca atactggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
    12781 tgtttgtgca tcagctgcgc gaacgcggaa ctcacggctc gctttggctg caataaatga
    12841 ccccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
    12901 gaccagaaat tgcagaatat tggccgttgg ggaagtgatt cctaacccaa acatgcgtcc
    12961 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
    13021 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
    13081 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
    13141 cagcaaaacg gcaaccaacg gccacacaga caaatgaaaa gtctcagcaa tagagttatg
    13201 ttcagtactg tagtgctgca aggtttgatt taaaccactg gcatgtggag aacgcatcac
    13261 cgcactcatc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
    13321 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcccagcc caaagatata
    13381 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
    13441 ctgaattaac gcaaaaaccc ccacactttg taccgaaatt gcaatcagca aggcgtaaaa
    13501 catgcgatta tttttcgcga gatacatatc gcgaaaacca cctgtcaggc aaaaacgccc
    13561 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaa
//
LOCUS       DAZFFI010000117        13537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFFI010000117 DAZFFI010000000
VERSION     DAZFFI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.117"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..515
                     /locus_tag="ACHJ12_004017"
     CDS             <1..515
                     /locus_tag="ACHJ12_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4991728.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            531..1070
                     /locus_tag="ACHJ12_004018"
     CDS             531..1070
                     /locus_tag="ACHJ12_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4991729.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1276..1764)
                     /locus_tag="ACHJ12_004019"
     CDS             complement(1276..1764)
                     /locus_tag="ACHJ12_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HFO4991730.1"
                     /translation="MIWVFLKAYWKQLIIIVVLVVLVISGVVAWNVHGSRQFDAGYAK
                     AQADQKQADDKFRSQREQEKTQIEREAQSRIDAARADAEYANTVADSMRAELDKTKRL
                     AEHYTGSFPSGTSASKVISVLADMLEESNRVYNATAVEAEKYRIAGEFCEQQYDSLKK
                     QK"
     gene            complement(1761..2390)
                     /locus_tag="ACHJ12_004020"
     CDS             complement(1761..2390)
                     /locus_tag="ACHJ12_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HFO4991731.1"
                     /translation="MEIKQFRRATGISEALAVRWFSHITSAMKEFGISKPEDQAMFIA
                     QVGHESGGFTRLQENFNYSVTGLANFVRAGRLTQWQANALGRRAGEPPLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLVKQPELLAQDEYAA
                     RSAAWFFSSKGCMKYTGDIARVTLIINGGRNGIDDRSARYITASKALMV"
     gene            complement(2380..2688)
                     /locus_tag="ACHJ12_004021"
     CDS             complement(2380..2688)
                     /locus_tag="ACHJ12_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4991732.1"
                     /translation="MTTHELLLLIANALICSAIAIRIVTFQRNGSQHRRWGGWLAYFL
                     IVASASIPVRAVYAIWYHIPMVVDLSELIINAVMFAAVFKTRGNVVQIFKITRSKDGD
                     "
     gene            complement(2675..3079)
                     /locus_tag="ACHJ12_004022"
     CDS             complement(2675..3079)
                     /locus_tag="ACHJ12_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO4991733.1"
                     /translation="MLTHPTSGSLNQWLSMGSLVAVIAGVPPEVALGALAGAVIFVTS
                     AIEYPVHRRVLLSMLSFLCGLLFYKPTASILIGIASLIPTITQDSFEKGVVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRDFIPGRKDDDNS"
     gene            complement(3126..5258)
                     /locus_tag="ACHJ12_004023"
     CDS             complement(3126..5258)
                     /locus_tag="ACHJ12_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFO4991734.1"
                     /translation="MTVSTEVDHNEYTGNGVTTSFPYRFRIFRNSDLVVQVSDINGNV
                     TILVLDTGYTVTGAGSYSGGSVVLPSPLADGWRITINRVLDVVQETDLRNQGKFFPEV
                     HEDALDYLTMLIQQCIGWFRRALIKPSLLARYYDAKQNRISNLAEPSLEQDAVNNRSM
                     RNYVDAAIAGVVGGFGWFIQYGSGAVYRTFQDKMRDTVSVRDFGAKGDGFSDDTPYIQ
                     LANDTATSIGATLHFPRGVYVCTDGIDKTCEWTGVGAAKIGVFPLNDDKVYMVPGNKH
                     KLPGTCLLFKGTGSKVFTTGRSDKFSSMRYCIRNVGRKDKGLTAFGGDFSVVCDFDFK
                     DQHGNITQPNNDNSADYDVGLILDNTDLSGPVNVTVGGYFKKSGIVHMGQDPDGCTII
                     NTRTMGNIGLAIIGDQTGTNSAFNMHGGAIFGNDHHSRNTEKGVEQWGEHALFIDIPS
                     ASGKGSRNGISFHGTWLATKLDVPVKYDRCGAVQYFGVVFENATQAGSQQAGGIKKQI
                     GTSNTGDIGFFGCRFNSDQIRISGALLETATESTVIVSGSNAGYGLEFWKGKVGGRFT
                     GSKNQTNIQLTDNPKITTSGIILRRDNTGNFHVMKDNDIKLSVDDKGIIANSSYTVVT
                     VSGGEITVTRNFHKLSGGTQELSTINGGNEGMRVILIRNTSTDNITLKNQTGNLRING
                     DFMLGAFDTIELIYAGGFWNEISRVNRT"
     gene            5454..5711
                     /locus_tag="ACHJ12_004024"
     CDS             5454..5711
                     /locus_tag="ACHJ12_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991735.1"
                     /translation="MQQYHYPLKDGFTERIHTPEGVRSLVEGSHLMALLRELDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTT"
     gene            complement(5733..6461)
                     /locus_tag="ACHJ12_004025"
     CDS             complement(5733..6461)
                     /locus_tag="ACHJ12_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991736.1"
                     /translation="MEVFWIVVGVVAVIIYALHQNKTKISDRTVVNHNKTIKTEDGEI
                     TINRTQVIEHTSTQFQKTGGNAPNISAPPAYDSAVIQTYYKQQELAKERQLIQPKPFT
                     AELPPGVSTRPAYHGRFPGDETSSQSPKKAPQAVSEPARIPSVSPSKEESANRVSSGR
                     KQCLRCRINLPYEKFRKSSKNPDGLTKWCARCLDGPKNTRHMKWCPICNVRRKRTSFY
                     PNNQNADGLMAWCKTCWDDHKAKR"
     gene            6856..7542
                     /locus_tag="ACHJ12_004026"
     CDS             6856..7542
                     /locus_tag="ACHJ12_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO4991737.1"
                     /translation="MTANVTPSVFHFESEATIRAIVIDGNPWFIAKDVIKALQLTNPT
                     MSIKSLDDDERAKFNLGRQGETNIINESGLYTLILRCRDAVTPGTIPYRFRKWVTGEV
                     LPQIRRTGSYIKNSLPQEERIKMVADQVASATASAVMQAMKVENKTYSAPLKPGYRSL
                     IHSPSGVLGLTENSLLMNLLNQLQDDGHDVSGAAAELTTMFCYIVGVSKCLRDIQTHA
                     EYINDKAGFF"
     gene            7664..7990
                     /locus_tag="ACHJ12_004027"
     CDS             7664..7990
                     /locus_tag="ACHJ12_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991738.1"
                     /translation="MSRRYVKELAIESGTPLGIVPGSNYAKALNRDSVVKVISRDMNM
                     VGDDLRRSENRVYAEYIVKNGKKIAAPAGGKWAIVTGKNSNARGKPEAKGMSFDKYAK
                     IYWATK"
     gene            7917..8465
                     /locus_tag="ACHJ12_004028"
     CDS             7917..8465
                     /locus_tag="ACHJ12_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO4991739.1"
                     /translation="MQEENQKLKECPSTNMQKSIGQPSDNEETEFEEVEEGLSNEIIQ
                     HPDAFTRVLDRPEIQEIVVAHHAFQGPLPPPYLLRGYQDILPDAPERIFQLTEKEFAH
                     RQKMEEKALDGAINRDKRGQWFGLSATIFTVACATLLGLTGHEVLAGTVIGTVVAVAG
                     IFVLRQKPSIKKKSEDNKPEEK"
     gene            8559..8720
                     /locus_tag="ACHJ12_004029"
     CDS             8559..8720
                     /locus_tag="ACHJ12_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HFO4991740.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(8763..8903)
                     /locus_tag="ACHJ12_004030"
     CDS             complement(8763..8903)
                     /locus_tag="ACHJ12_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991741.1"
                     /translation="MIAFILVVFALVALGVMNRKCIIDDGEFAVAVILILSGVAGYIG
                     LS"
     gene            complement(8913..11387)
                     /locus_tag="ACHJ12_004031"
     CDS             complement(8913..11387)
                     /locus_tag="ACHJ12_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991742.1"
                     /translation="MRQECIQAVQQAAHRTLTAREIQNIEDRIYRNMRSIARDDPMSW
                     RQLSESERLYRAAQLASEELQREAALKKRRVALTIAARQRLDKFINSYQGADGKLGAL
                     NRTIAFNADGKSNFLSVESRTKATRDYALSQLQEAFEAVDPRFFGLFEDEAGVRDLVY
                     EMRGQNTGNAKARKGAKAWREVTELLRRRFNDAGGDIGYLENWGIPQHHSMEKVGAVS
                     KDKWVGDVIGKLDRKYYTRADGQLMNDAELSAFLGEAYNTIATGGLNKLTDTGMRISG
                     ARANRGNASRQIHFKDADSYLQYQQLYGDRSLWEIMVGHLEGISKDIALVETYGPNPD
                     HVFRSLLDQVKAETATANPSKTGKVERLANNTENLYNFISGKTQPVANPHIARWSDNI
                     RNWLVASRLGSALLSSFSDLGTMYLSAKVTNLPMNQLFRNQLEAMDPTNRTELARARR
                     AGLAMESLLGSVNRWAMDNMGPSVSRWAATAVMRASGLTAWSDAHKRAYGVTMMGSLG
                     EVVSRTPDLRSLDDSDFRILKSKGITDTDWSVWKLAQQEDWGNGNNTMLTPESIMRIP
                     DSAVKHLGEPERVKFEAMRKLLGAVTEEVDMAVITPGAREQMFVGSGLQRGTWKGELT
                     RSVFLFKSFPISVVMRHWSRAMGMPSAGGRAAYIATFLASTTMLGALSMQITDLINGR
                     NPKEMTGDHMVKFWINAFLKGGGAGLYGDFLFSDHTRYGSGALASMLGPVAGLVDDVV
                     KIAQGIPLNAVEGKNEQTGGDLVKLGKGLMPGANLWYLKAALDHMIFNQMQEYFSPGY
                     LRKMEQRSKKEFNQTYWWRPQDVAPQ"
     gene            complement(11393..13195)
                     /locus_tag="ACHJ12_004032"
     CDS             complement(11393..13195)
                     /locus_tag="ACHJ12_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991743.1"
                     /translation="MSYFGLNPVNQNQQLDEAASNPVGFNSDVGFFDNAVGAALSGLY
                     SGLVAKPDQLLWAGMDKIVSPIAQFVNENTSLNDTSVSYIAEQRKLAEQQVKRLTPDA
                     ATTGTAGQVLYGLFDMGGQAVVGTTLGGPVGGAAAVTSLQGFSEFERLTAQGVDFRTA
                     QEAGLVQGITAGAGTLIPMSLGLRAGGALAEGVAAQLARTGESSVRRAAATAVRATPD
                     IAYAAGTNIAFGMAQRGLTAKTLRDGGYSEMANQYDVLDRQAIAIDAVLGVAFGGVGR
                     FINSRGESTSAPNFSPVDIDAALAANAAHHAEIDIAPGVPINVLSRNSHIQALRKAMS
                     DVSQGRPVDVASIVESASFSEIPGRKSLLSQAVNEALSSVDDGVTARAIENRLLEEQA
                     AQLLPRGDRQVYQSEIANSQRIIENLTEQRAQILAEEPTGSGKALSRARSDKQARLRD
                     IEQRIRQAQERLEFSRNALAPHEPGGQFFEARAELARRQQAESELNAQAVSFYKTAEV
                     RTPDEVVPFEPDKILQQAEQKMMADQAGDIDLRIAEDSLLESPDMIITVLDDDGNPQS
                     RSAREVLDEANRESEQAIQDSSLFDVAVACFLRG"
     gene            complement(13192..>13537)
                     /locus_tag="ACHJ12_004033"
     CDS             complement(13192..>13537)
                     /locus_tag="ACHJ12_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991744.1"
                     /translation="AKKGVVSGEIDNDIWKQAVNVATGGVHDYNGMGNVLLPWGMSAE
                     QFDKQVNQAWNEQVVGTGIKTPPGQYGLQSYGDSQYLVKLGTGYLLKDDGSPVVLDLT
                     QKRQRFSGDIPQ"
BASE COUNT         3700 a         3432 c         2897 g         3508 t
ORIGIN      
        1 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
       61 gccagtcaaa tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      121 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      181 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      241 gtgttatcgg ccacaacgtc ggttctggca gcaattccgg aacgacggca ggggcagttg
      301 gcggcggagc tgtaggcgcg gcagtgggtt ctatggtgaa tgataaaacc ttagtggaag
      361 gtgtttcttt aacatataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag
      421 agtgccagtt tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta
      481 ttcagccaaa tactaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      541 tttttctttg cgccatcttg gcctccttaa gctatccggc tatcgcctca tcattgcagg
      601 atcaactctc tgctgtagca gaagctgaac agcaaggtaa aaatgaagag caaaggcagc
      661 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      721 atgcccaggc cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      781 aggataaact ggacgccgaa gcctctgcgg acaaaaaacg cgatcaaagt tatgaagatg
      841 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcaaaagaa gaagcccgcg
      901 ttaagcgaga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
      961 tggtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1021 aaagcgtggg caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttag
     1081 taacttcaag cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1141 tcattcatag tgcagggatt aaaatcattc ccactcaatt atttactgat accataaaca
     1201 attgattaat aacatttttc ctaaacttca ttttgatcat accgttttat ataccgtcac
     1261 cgaaaatcag tacccctatt tttgcttctt caatgagtcg tattgctgct cgcagaattc
     1321 ccctgcgata cgatactttt cagcctcaac tgctgttgcg ttgtaaactc ggttgctttc
     1381 ttcaagcatg tcggcaagca cactgatgac cttgctggct gacgtgccag aggggaaaga
     1441 tccggtatag tgttcggcga gtcgcttggt tttgtcaagc tcggcgcgca tgctgtcagc
     1501 aacggtatta gcatactcag catcagcacg cgccgcatcg atacgggatt gtgcttcacg
     1561 ttcaatttgt gttttctcct gctcacgctg tgacctgaac ttatcatcag cctgtttctg
     1621 atctgcctgt gccttcgcat aaccagcgtc gaactgacgg ctgccgtgta cattccaggc
     1681 aacaactcct gatatgacca gaacaacaag caccacaatg ataatcaact gtttccagta
     1741 tgcttttagg aatacccaga tcataccatc agcgccttac tggcagtgat gtaccgcgcg
     1801 ctccggtcgt cgatgccgtt cctgccacca ttgataatca gagttacacg tgctatatcg
     1861 ccggtatact tcatgcagcc tttgctggag aagaaccacg ccgcgctacg agccgcatat
     1921 tcatcctgcg ccagcagttc aggctgctta accagatcca cctttaatcc gttgccacag
     1981 tcacgatagt tattcagacc ggtaatctgg ataagtccgc gcccacgata attccagcca
     2041 tcgccgggag cattgttccc catgcgtttg ctgtacacca gattggcgat cgctctctgc
     2101 cgctcaagtg gcaatggtgg ttcaccagca cggcgaccca gtgcattagc ctgccactga
     2161 gtgagacgac cagcccgaac aaagttagcc agtccggtga cgctgtagtt gaaattctcc
     2221 tgcaacctgg tgaagccccc agactcatgc ccgacctgag caataaacat tgcctgatct
     2281 tcgggtttgc tgattccaaa ctctttcatc gcagaagtta tatgcgagaa ccagcgtacg
     2341 gccagagcct cgctgatacc agttgcgcgc cggaattgtt taatctccat ctttagacct
     2401 cgttattttg aaaatctgaa cgacgttacc gcgagtttta aaaaccgcag caaacatgac
     2461 ggcgttgata atgagttctg ataaatccac aaccattggt atgtgatacc agattgcata
     2521 tacagcgcgg acagggatgc tggctgacgc cacaataagg aagtaggcta gccacccacc
     2581 ccacctccgg tgttgagatc cattacgctg gaaggttacg atgcgtattg ctattgcaga
     2641 acagataagc gcattagcaa tgagtaaaag cagctcatga gttgtcatcg tcttttctcc
     2701 ccggtataaa atcgcgtgga ttgtctgaac gatgatagag ccagatacca atacgtactg
     2761 cgacaattgc cgacacgaac gcaccagcag agaagacgac ccctttctcg aatgaatcct
     2821 gcgtgatagt tgggatcagg ctggctatgc cgataagaat tgatgcagtt ggtttgtaaa
     2881 agagaaggcc gcagaggaag ctgagcatcg acagcagaac ccgtcgatga acaggatatt
     2941 ctatggccga ggtaacaaaa attaccgccc cagccaaagc ccccaaagcc acctcaggag
     3001 gaactccagc aatgactgct acaagagatc ccatactaag ccactgattt aaagaaccac
     3061 ttgttggatg agttaacata cataaaccat atataaataa aaataacttc tattttcaaa
     3121 agaagttaag tgcgatttac gcgagaaatc tcattccaga atcctccagc gtagattaat
     3181 tcgatagtgt caaaagcacc taacatgaaa tcaccattga tacgcagatt tcctgtctga
     3241 ttcttcaacg ttatgttatc agttgatgta ttacgtatga gaatgaccct catgccttca
     3301 ttaccaccat tgatagtcga taactcctgt gtaccaccag ataatttatg aaagtttctg
     3361 gtaactgtaa tttcgccacc agatacagtg accacagtat aagatgagtt agcgataata
     3421 cctttatcat caactgataa tttaatgtca ttatctttca ttacatggaa atttcctgta
     3481 ttgtcccgac gaagaataat accgctagtg gtaatttttg gattatctgt tagctgaata
     3541 ttggtttgat ttttacttcc agtaaagcgt ccaccaactt ttcctttcca aaactccaat
     3601 ccatagccag cattactacc agagacaatc acagttgatt ctgtagctgt ttccagtaaa
     3661 gcacctgaaa tacgaatctg gtctgaatta aatctgcatc caaagaaacc tatatcacct
     3721 gtattactgg ttccaatttg ttttttaata cccccagctt gttggctacc tgcttgtgtt
     3781 gcattctcaa acaccacacc aaaatactga acggctccac atctgtcata ttttacagga
     3841 acatccaact tagtcgctaa ccaggttccg tgaaaagaaa taccgtttct ggatcctttt
     3901 cctgaagctg aaggaatatc aataaaaagg gcatgctccc cccattgttc aacacctttt
     3961 tcggtattac gactatgatg atcattgcca aaaatagcgc ctccgtgcat attgaatgca
     4021 ctgttagttc ctgtttgatc accaataata gccaggccaa tattacccat tgtacgtgta
     4081 ttgataattg tacaaccatc gggatcctgc cccatgtgta caattccaga ttttttaaag
     4141 tagccaccta cagtaacatt caccggccct gacaaatcag tattgtcaag tatcaaccca
     4201 acatcataat cagcagaatt atcattatta ggttgagtta tattcccatg ttgatcttta
     4261 aagtcaaaat cacaaacgac agaaaaatcc ccaccaaatg cagtaagccc tttatctttt
     4321 cgacctacat tacggatgca gtaacgcatc gatgagaatt tatcgcttct acctgtagta
     4381 aataccttgc taccagtacc tttgaataaa aggcaagttc caggcaattt gtgcttattt
     4441 cctggaacca tataaacttt atcatcgttt agtggaaata cacctatttt tgccgcacca
     4501 acaccagtcc actcgcacgt tttatcaatc ccatcagtac aaacgtaaac acctcttggg
     4561 aaatgtaatg tggcaccaat tgatgtggct gtatcatttg ccagctgaat atatggggta
     4621 tcatcactaa aaccatcccc cttcgcccca aaatcgcgaa cactgacggt atcccgcatc
     4681 ttatcctgga acgttcggta tactgcccca gaaccatact gaataaacca gccaaaacca
     4741 ccaacaaccc cggcgattgc agcatcgaca taattacgca ttgagcgatt atttacagcg
     4801 tcctgctcaa gtgatggctc tgcaaggtta gatattctgt tttgctttgc atcgtaatac
     4861 ctggcaagca acgaaggttt tatgagcgct cgcctgaacc atccgataca ttgctgaata
     4921 agcatggtca gatagtcaag tgcatcctcg tggacctctg gaaaaaactt cccctgatta
     4981 cgaaggtctg tctcctgcac tacatcaagt acacggttta tagtgattct ccagccatca
     5041 gcaagcggag atggcagaac cacagaaccg ccactataac tacctgcacc agttaccgta
     5101 taaccagtat ccagtaccag aattgttaca ttcccgttta tgtccgatac ctgaaccaca
     5161 aggtctgaat ttctgaaaat tcgaaacctg tacgggaatg atgtcgtaac cccgttacca
     5221 gtgtattcat tgtggtcaac ttcggttgag actgtcatgt taaatctcca gatagtcgca
     5281 gcacctgttg cgccgcatat ctggttatag tattaccata aaaaccatat atgaataaaa
     5341 ataatataaa aacaaaaatc tattaccttt tgggtaattt taaaaaccta gtagatagaa
     5401 aaaacataat ttgttactgt ataaatacac agctatcaca tggagaagat aagatgcagc
     5461 agtatcacta tccactgaaa gacggattta cggaaagaat tcatactccg gaaggcgtca
     5521 gatcactggt ggagggatcg cacctgatgg cattactccg ggagcttgat aaagatggtt
     5581 ttaatgtcga tggcccactt gccgaactga ctgcactgat taactacgtc accagctcac
     5641 agatgtctat gcaggatctg caaacacatc tcgactattg tgccgaacaa ttacgaaaac
     5701 aaactacata aagaaaaggc cgcaagagcg gcctatcgtt tcgctttgtg atcgtcccag
     5761 cacgttttgc accatgccat taaaccatcc gcattttgat tattaggata aaagctggtt
     5821 cgttttctgc ggacattaca aattgggcac cacttcatat gacgtgtatt ctttgggcca
     5881 tcaagacacc ttgcacacca cttagtcaat ccatctggat tttttgacga tttccggaat
     5941 ttttcatatg gaaggtttat tctgcatcgc aaacactgct tgcggccact tgaaactctg
     6001 ttagctgatt cttcttttga cggcgataca gaaggtattc ttgctggctc tgatactgcc
     6061 tgaggtgctt ttttaggtga ctgagacgat gtctcatcac cagggaatct tccatgatat
     6121 gccggacgcg ttgacactcc aggtggaagc tctgctgtaa atggctttgg ctgaatcagt
     6181 tgcctctctt ttgctaactc ctgctgttta taatatgtct ggattaccgc actatcataa
     6241 gcaggaggtg cggaaatatt aggcgcatta cctccagttt tttgaaactg agtagaggtg
     6301 tgttctatca cctgtgtacg attaatcgtt atctccccat cttcggtctt tatcgttttg
     6361 ttatgattaa cgaccgtacg atcagagatc ttagtcttgt tctggtgaag ggcataaata
     6421 atcaccgcaa ccacaccaac aactatccag aaaacttcca ttgcttttcc tcacaataac
     6481 attaccttaa aggtaatatc ttgctttcag gtgatcaagc gttaaacgta acaaaccaaa
     6541 tgcggttgat tttgatattt ctccgcgttt atcattacct ttgcggtaaa tttacatcgc
     6601 actcctcttg tgccatagta atcgggcact ggcaaaatcc agtgccggga ttggcgtccc
     6661 gggttactac agaggcacat atgccgcata agcggttttt ttatgtgtaa agcgcaccta
     6721 ttctatggtg ggctgtgtgg gggcaccgaa aggtgcgccg ggtcctttgt agccggttac
     6781 gccaaccctg cacagttcac caccaaccga ttggcgtcgg tagtggtgat taacctaact
     6841 acaaaggtga tcactatgac tgctaacgta accccatctg tttttcattt tgaatcagaa
     6901 gcaaccattc gagccattgt tattgatgga aatccttggt ttattgccaa agacgttatt
     6961 aaagctcttc aactgacaaa ccccactatg tcaataaaat ctcttgatga tgatgaaagg
     7021 gctaaattta acttaggccg tcaaggcgaa accaatatta tcaacgagtc aggcctctac
     7081 acactgatcc tccgctgccg cgacgcagtg acaccaggca ctatccccta ccgctttcgt
     7141 aaatgggtta caggtgaggt tcttcctcag atccgccgca ccggaagtta cattaaaaac
     7201 tcgctcccgc aggaagaacg cataaagatg gttgccgacc aggtagccag cgccacagca
     7261 tcagcagtga tgcaggcaat gaaggtagag aacaaaacct acagcgcccc actgaagccc
     7321 ggctaccgca gcctgattca ttcgccgtct ggtgttcttg gcctgacgga gaactcactg
     7381 ctgatgaatc tgctgaacca gttacaggac gacgggcatg acgtatcggg cgcggcggcg
     7441 gagctgacaa ccatgttctg ctacatcgtc ggtgtgagca aatgcctgcg tgatatccag
     7501 acccacgcgg agtacatcaa cgacaaggca gggttcttct gacgggcggt ggcacaggga
     7561 tgtgcctaca attatcaata attgtttaca ctcgttgcag ttaaaagcat tatactcatg
     7621 tttaaaatta cccgattggt aaagtcgatt ggttgaggtt catatgagca gaagatatgt
     7681 caaggagctg gctatagagt ctggcacccc tttgggtatc gttcctggct ccaactatgc
     7741 caaggcttta aaccgagatt ctgtcgtcaa agttatatca agagacatga atatggtcgg
     7801 agacgatctc agacggtcag aaaatcgtgt ttatgctgaa tatatagtaa agaacggaaa
     7861 aaaaattgca gcgcctgctg gcggaaaatg ggcaatagtg acagggaaaa actcaaatgc
     7921 aagaggaaaa ccagaagcta aaggaatgtc cttcgacaaa tatgcaaaaa tctattgggc
     7981 aaccaagtga taacgaagaa actgagttcg aggaagttga ggaagggcta agtaacgaga
     8041 tcattcaaca cccagatgcc ttcacaagag ttcttgatcg ccctgaaatt caggagatag
     8101 ttgtcgctca ccatgcgttt cagggtccgc ttcctccccc ctatctgctt cgcgggtatc
     8161 aggatatttt acctgatgcc cccgaacgca tcttccagct gacggagaag gagtttgctc
     8221 accgccagaa aatggaggaa aaggcacttg acggggctat taatagagat aaacgcggac
     8281 agtggtttgg cctgagtgca actattttta cagtagcttg tgcaacatta ttaggtttaa
     8341 caggtcatga agttctagca ggcacagtga tcggtacagt tgttgcagtg gctggtattt
     8401 tcgttcttag acaaaaacca agcatcaaga agaaatctga agacaacaaa cctgaagaaa
     8461 agtaagcccg cgctgcgggc ttttttgtga acgaaacaaa agtcagtgct acactcattg
     8521 acgccacatt gaggtggctt atagatggaa atttcacaat gaaaaaagca tttgctgcac
     8581 tgttcgtttt gttgtctctg gtagcttcaa ctcaggcctt tgccggtcgt tgtcagcacg
     8641 acagcgatac tgccgctgac ggctcccgct gcggtgggcg ttctgcggat tcccgcccag
     8701 gtggcggtgg cattcgttaa aaacaaggcc gcgaaagcgg cctgtgacat gtcacgctca
     8761 cgttatgaca agcctatgta ccctgctaca ccagataata tcaaaataac tgcaacagca
     8821 aattcaccat cgtcaatgat acatttacgg ttcataacgc caagtgcaac aagcgcaaac
     8881 acaacaagaa taaaagcaat catttctcat ccttattgcg gagcgacatc ctgaggtcgc
     8941 caccagtatg tctggttaaa ctctttcttc gaacgttgct ccattttacg caaatagcct
     9001 ggtgaaaaat actcctgcat ctggttaaag atcatgtgat cgagagccgc cttcaagtac
     9061 cagagattcg caccaggcat tagacccttc cccagcttaa ccagatcacc accagtctgc
     9121 tcattcttcc cttccacagc atttagaggt atgccctgag ctatcttcac tacgtcatca
     9181 accagaccag ctaccgggcc aagcatcgac gccagcgcgc cgctcccgta cctagtgtgg
     9241 tcagagaaaa gaaaatcacc gtacaaccca gccccaccac cttttaaaaa tgcatttatc
     9301 cagaatttaa ccatgtggtc accggtcatt tcctttggat ttctcccatt aataagatca
     9361 gtaatctgca tggaaagagc accaagcatg gttgtgcttg ctaaaaacgt tgctatatat
     9421 gctgcacgcc caccagcaga cggcataccc atagcgcgtg accagtgacg cataacaacc
     9481 gagataggga acgatttaaa caggaaaaca cttctcgtta attcaccttt ccatgttcca
     9541 cgctgaagac cagaccctac gaacatctgt tcacgtgcgc ccggtgtaat aacagccata
     9601 tcaacttctt cagttacggc accgagcagt ttacgcattg cctcaaattt cacgcgttca
     9661 ggctcaccaa gatgtttaac tgctgaatca gggatacgca taatgctttc cggtgtcagc
     9721 atcgtattat taccgttccc ccagtcctcc tgttgcgcca gcttccatac gctccagtct
     9781 gtgtcagtaa tccctttgct tttcaggata cgaaaatcag agtcatcgag gctacgaagg
     9841 tctggtgtcc gtgacactac ttctcccagg cttcccatca tggttacgcc ataggcgcgc
     9901 ttgtgcgcat ctgaccatgc tgtaagccca ctggcacgca ttaccgccgt tgccgcccaa
     9961 cgagacactg acggccccat attatccatc gcccagcggt taacgctgcc aagtagagat
    10021 tccatcgcca gaccagcgcg gcgcgcccgc gcaagttctg tacggttcgt tgggtccata
    10081 gcttcaagct ggttgcggaa taactggttc attggaaggt tggtaacctt cgcagacaga
    10141 tacatggttc caagatcaga gaacgatgac agcaacgcgg atccgagtct gctggcaacc
    10201 agccagttgc ggatattgtc agaccatcgc gcgatgtgcg gattcgctac aggctgtgtc
    10261 tttccggaaa taaagttgta cagattctct gtgttgttcg ccagccgctc gactttaccg
    10321 gttttactcg ggttagctgt tgccgtttct gccttaacct gatcaagaag ggagcggaaa
    10381 acatgatcgg ggtttggacc atatgtttcc accagagcaa tatctttact gataccttcc
    10441 aggtgaccga ccatgatttc ccatagagag cgatcaccat aaagttgttg atattggaga
    10501 taggaatctg catctttgaa atgtatctgt cgtgatgcat taccacggtt agcacgtgcg
    10561 ccggaaattc gcattccggt atcagtaagc ttattcagcc caccagtagc gatcgtgtta
    10621 taagcctctc caagaaatgc agacaactcg gcatcgttca tcagttgtcc atcggctcgg
    10681 gtataatatt tgcgatccag cttacctata acatcgccaa cccacttatc ctttgatacc
    10741 gccccaacct tttccataga atgatgttga gggatccccc agttttcgag atagccaatg
    10801 tccccaccag catcattaaa ccggcggcgc agcagctctg taacttctct ccacgcctta
    10861 gcaccttttc ttgctttagc attgccagta ttttgccccc gcatttcata taccaggtca
    10921 cgtacgcccg cctcatcttc aaacagacca aaaaagcgag gatcaactgc ttcgaatgcc
    10981 tcctgtaatt gactcaatgc ataatcacgg gtggcttttg ttctggattc aacagagagg
    11041 aaattagatt taccgtctgc attaaaagct atagtacggt taagagcgcc aagtttccca
    11101 tcagcccctt gatagctatt gataaattta tccaatctct gacgcgcggc tatagtgaga
    11161 gccacacgac gtttctttaa tgccgcttct cgctgtaatt cttcagatgc caattgtgct
    11221 gctcgatata gccgctctga ttcggaaagt tgtcgccatg acatcgggtc atcacgagca
    11281 atggagcgca tatttcgata aatgcggtct tcaatgttct gtatttctcg cgccgttaac
    11341 gtgcgatgcg ccgcctgctg gaccgcttgt atacattcct gtctcattta atttaacctc
    11401 tcaagaaaca cgccacagct acatcaaaca ggcttgaatc ctgtattgcc tgctcacttt
    11461 ccctgttcgc ttcatccagt acttcacgcg cgctgcgcga ttgtggatta ccatcatcat
    11521 ccagcacggt gattatcatg tcaggtgatt caagcagcga gtcttcagct atgcgcagat
    11581 caatatctcc cgcctgatcc gccatcattt tttgttctgc ctgttgcaat attttatcag
    11641 gctcaaaagg aactacttcg tctggcgtcc tgacctctgc tgttttatag aatgaaacag
    11701 cctgagcatt aagttcactt tctgcctgtt gtctccgagc cagttctgct cgagcttcaa
    11761 aaaactgacc gccaggctcg tgcggtgcca acgcgttacg ggaaaattcc aggcgttctt
    11821 gtgcctgccg gattcgttgc tcaatatcgc gaagtctggc ctgtttatct gatcgagcac
    11881 gagacaaagc tttaccgctg ccggttggct cttctgcaag aatttgtgcg cgctgttcag
    11941 tgagattttc aataattcgt tggctattag cgatttcaga ctggtaaacc tgtctatcgc
    12001 cacgcggcaa aagctgcgcg gcctgttctt caagcaaccg attttctata gcgcgcgccg
    12061 ttactccatc atctacagat gacagagcct cattaactgc ctgagacagc agactcttgc
    12121 gcccaggaat ttcactgaaa gatgcagact caacaatgct ggcaacgtct acaggtctcc
    12181 cctggctaac atcagacata gcttttcgca gagcctgaat gtgagaattg cgcgaaagca
    12241 cgttgatcgg cacgccgggc gcaatatcaa tttcagcatg atgagcggca ttcgccgcca
    12301 gtgcagcatc gatatcaact ggtgaaaaat ttggtgcgct tgtagactcg ccgcgtgagt
    12361 taataaatct gccgacacca ccaaacgcca ccccaagaac agcatcaata gcaattgcct
    12421 gtcgatccaa cacatcatac tggttagcca tttcgctata gccaccatca cgaagcgttt
    12481 ttgcagtaag cccacgctgt gccataccga acgcaatatt tgtacctgcg gcataggcaa
    12541 tatctggcgt tgcacgtact gctgttgctg cggcgcgtcg cactgaactc tcacccgtcc
    12601 gcgcaagctg agccgccaca ccttccgcca gcgcaccacc agcacgtaac ccgaggctca
    12661 tagggatcag tgttccggca ccagcagtaa tgccctgcac taatcccgct tcctgcgccg
    12721 tcctgaaatc aacaccctgt gctgtcagcc gttcaaactc agaaaaaccc tgtagcgaag
    12781 ttaccgccgc tgcacctccg acaggaccac cgagcgttgt accgacaaca gcctgcccgc
    12841 ccatatcgaa caacccataa agaacctgcc cggcggttcc ggttgtcgcg gcatcaggcg
    12901 tcagccgctt aacctgctgc tctgctagtt ttctctgctc agcaatgtat gaaactgaag
    12961 tgtcattgag cgaggtgttt tcgttaacaa actgagcaat cggggatacg attttatcca
    13021 tccctgccca tagcaactga tctggctttg ccaccagccc ggagtacaaa ccagacaatg
    13081 ccgctcctac agcattgtcg aaaaaaccaa catcgctgtt aaagccaact ggatttgatg
    13141 ctgcttcgtc aagctgctga ttctggttta ctggattaag gccaaagtaa ctcattgcgg
    13201 aatatctccg gagaatctct gacgcttctg tgtcagatca agaacaacag gagaaccatc
    13261 atctttcagc agataaccag taccaagttt caccaggtac tgactatcgc cgtaactttg
    13321 caaaccatac tgaccaggcg gtgtttttat ccctgtgcca acaacttgtt cattccaagc
    13381 ctgattaacc tgcttatcga attgctctgc agacattccc cacggcaaaa gaacattccc
    13441 cattccgtta tagtcatgca cgccacctgt agctacgtta acagcctgtt tccagatatc
    13501 attgtcaatt tcgcctgata ccacgccttt tttcgcc
//
LOCUS       DAZFFI010000118        13444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFFI010000118 DAZFFI010000000
VERSION     DAZFFI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.118"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..346)
                     /gene="cspH"
                     /locus_tag="ACHJ12_004034"
     CDS             complement(134..346)
                     /gene="cspH"
                     /locus_tag="ACHJ12_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4991745.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            632..844
                     /gene="cspG"
                     /locus_tag="ACHJ12_004035"
     CDS             632..844
                     /gene="cspG"
                     /locus_tag="ACHJ12_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4991746.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            855..1043
                     /gene="ymcF"
                     /locus_tag="ACHJ12_004036"
     CDS             855..1043
                     /gene="ymcF"
                     /locus_tag="ACHJ12_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4991747.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            1018..1248
                     /gene="ymcE"
                     /locus_tag="ACHJ12_004037"
     CDS             1018..1248
                     /gene="ymcE"
                     /locus_tag="ACHJ12_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4991748.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLQACCCLLRPEEVNSEY"
     gene            1238..1411
                     /gene="gnsA"
                     /locus_tag="ACHJ12_004038"
     CDS             1238..1411
                     /gene="gnsA"
                     /locus_tag="ACHJ12_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4991749.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1459..2532)
                     /gene="yccM"
                     /locus_tag="ACHJ12_004039"
     CDS             complement(1459..2532)
                     /gene="yccM"
                     /locus_tag="ACHJ12_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4991750.1"
                     /translation="MAENKRIRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2604..5348)
                     /gene="torS"
                     /locus_tag="ACHJ12_004040"
     CDS             complement(2604..5348)
                     /gene="torS"
                     /locus_tag="ACHJ12_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4991751.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5431..6459
                     /gene="torT"
                     /locus_tag="ACHJ12_004041"
     CDS             5431..6459
                     /gene="torT"
                     /locus_tag="ACHJ12_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4991752.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDIVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6432..7124)
                     /gene="torR"
                     /locus_tag="ACHJ12_004042"
     CDS             complement(6432..7124)
                     /gene="torR"
                     /locus_tag="ACHJ12_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4991753.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7254..8426
                     /gene="torC"
                     /locus_tag="ACHJ12_004043"
     CDS             7254..8426
                     /gene="torC"
                     /locus_tag="ACHJ12_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4991754.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRTELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8426..10972
                     /gene="torA"
                     /locus_tag="ACHJ12_004044"
     CDS             8426..10972
                     /gene="torA"
                     /locus_tag="ACHJ12_004044"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4991755.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10969..11568
                     /gene="torD"
                     /locus_tag="ACHJ12_004045"
     CDS             10969..11568
                     /gene="torD"
                     /locus_tag="ACHJ12_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4991756.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11661..11966)
                     /gene="cbpM"
                     /locus_tag="ACHJ12_004046"
     CDS             complement(11661..11966)
                     /gene="cbpM"
                     /locus_tag="ACHJ12_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4991757.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11966..12886)
                     /gene="cbpA"
                     /locus_tag="ACHJ12_004047"
     CDS             complement(11966..12886)
                     /gene="cbpA"
                     /locus_tag="ACHJ12_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4991758.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13146..>13444
                     /locus_tag="ACHJ12_004048"
     CDS             13146..>13444
                     /locus_tag="ACHJ12_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991759.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGV"
BASE COUNT         3328 a         3372 c         3487 g         3257 t
ORIGIN      
        1 aactattagg tgcagagata ttcgcttaaa tggattagtt tctttgtgga aaagtaactg
       61 atgttataat gtatgatgtt gactattgca ctattccaat aaaaccagtc agctttaaac
      121 aagcagcgtc atattaagag agataaacat ttgccgctgt tggtcctcgc aggccattta
      181 cgcggcaaaa ttccacacgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg
      241 cggaaatatg gacctgaact tctttacgac cgtcggaggg gataatgaat cctttgccgc
      301 ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact
      361 gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata tttaagaccg
      421 ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg
      481 tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta
      541 tccccttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa agccctgttt
      601 gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg gtttagtaaa
      661 atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt
      721 tttcgtccat ttcaccgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa
      781 agttgaattt tctattgagc aggggcaacg tggccccgcg gcagcgaacg ttgttacgct
      841 ctaaggttgc cgttattact caacatctcc atttccgctg tccatgttgt catggttcac
      901 agtaccgcac atcggcattc gatgtgacgg agcgaaaccc tttgggcgct aagtgtattt
      961 tttgtaaatc gacgatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg
     1021 tgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc
     1081 ttatttttct tcaacgctgt atgtatagta agcgataacc tgttgattat tgaatctttc
     1141 ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa caccttacag
     1201 gcgtgttgct gtcttttaag accagaagag gttaacagtg aatattgaag agttaaaaaa
     1261 acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc tgaacaagaa
     1321 tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga ccgggcttga
     1381 aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcacccgt gcgaggtgat
     1441 gttccgcttg ttgctaattt agtgaccaat cattggcgct tgtggaatta agcgtcggta
     1501 caattcatcc ggtaccgggc tttgccatac tcccgcatac attgcgtaac caatcaccgc
     1561 aaacataatc cccagaacca gtagcgtcat taaccagcca gacaacgcaa aggctttttt
     1621 atttgccgca ggtttttgca gtgaaaaggt caatgttgag gctaccggac atgactctac
     1681 gcaagtcata cagccggtac attccactgt tcgtacctga attaatttat cgaccgggat
     1741 ccgtgatggg caattttttg cgcatttgcc acagtcgata caactttcgg cattgcgacg
     1801 aatcttaaac ggcgacaata gcgaaaccac gcccatcagc gcgccatatg ggcaaagata
     1861 acgacaccag gcatggcgaa taaacaggct ggcaatcagc aaaacggtca cgctgattaa
     1921 tgtcgcggtc cccatatgac gaaagaaatc gagcatttta acgtccatca ccacgctgta
     1981 gggcgacaac ataaaatagt gaatcgcctg agcgggcatc aataacgcga tatagagaaa
     2041 aaaactcaac agcaaatact tcacgccgcg cagaggaata tccagccagc ggggaaggac
     2101 acattgccga ccaaacagtt tgttaccgag atcgccgatt aattcagaaa gcgtaccaac
     2161 cgggcataac catgagcaaa aggccttttt gagtaataga ctgatgacga taaaagcgac
     2221 caataacagc atcgcggcgg cgtggacgga cggtaactga cctgttacaa ggctatattt
     2281 cagattcatc agcccggcaa tcggtagcca gccttcgata cctcccggtc tggcgacaaa
     2341 tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac cagaacgtga tggcaatata
     2401 aatattcatt gccaacagta ataattgcgt cgctttacgc caggtcgtgg cattacgcca
     2461 gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc ggattctttt
     2521 attctctgcc atgattttgc cagtccgtta agttgtatac caaatgccac tattctagtt
     2581 gttcttaact ggctgatatt gattcaaatc gcgttcaggt ctttcttatg caaccatgct
     2641 tccagagcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc
     2701 tgctccagtt gtgcgcatag ctggctggcg atacgcattc ccagactcga gcaactgctt
     2761 tttagctgat gcgcggcacg ctttattttc tcgctatcct gactggcgcg ggcaatgtcg
     2821 atttcatcga gaagcggcag ggcatgttgt gtaaataata ccagccattc gtggatcttc
     2881 tccgtcccca ttaactgagc atcttcattg agttgcgata catccagcga ttgatcgtta
     2941 ttgacttgca gttggagata gtgcgccagt aactgaccga gcacttcacg cggcaccggt
     3001 ttagggataa tcccgcggaa taatgaactg gtacgctggc gcagcgtttc gtcaatgaca
     3061 tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg tcgggcaagc
     3121 gtaatgccgt cgacatccgg cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta
     3181 ttttgcagtg tctctaaagc ctgcgcggca ttgccaacag caacaacctg cgcaccactg
     3241 gtgttcagca tctcaacggt aattcgctgg gttagcgggt tatcttcaat taacagcaaa
     3301 cgtaaaccgt caagacgcac cgcctgattg actgtttttg gcacgggtgc cgtggcaaca
     3361 cgtaacggca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt
     3421 tcgccgccca tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg
     3481 cgtttgccgc ttacctgcac aaatggctgg aagatttctg ccagtttcgc gggatcaata
     3541 ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc catcagtgcg actacgcagg
     3601 ataatgtacc cttcgtcagt aaaacgcagg gcgttgctca acaggttggt tataacctga
     3661 cgaatacgtc gtggatcgcc cattaacgcg cacggcatat catcggcaat tgccgttgcc
     3721 aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcagc
     3781 gggcgcggtt caaagggctc atcgctgacc gaaacattct tgccacctgc ttcgatagcg
     3841 gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc agtaattgcc
     3901 cgcaaatcat cacgctgggc gttaagtgcg gggttatctg ccagcagttg agcagtgccg
     3961 agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt
     4021 gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc
     4081 aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg attcagtgca
     4141 tgaacattgc tgcgaaacgc atccatcagc cgcccgatgg tatccagctc ccgtacgcca
     4201 gcggtttccg ggaaagggga gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt
     4261 tgttcggcaa gtggacgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt
     4321 gcgcaaagag aaactattcc cagcaataac aggctgtatt gcccgcgtgc actcgctttt
     4381 tccagatgcg ccagtccgtg ctgattacgc agctcaatgg tatcgaccag ctgactgact
     4441 tcgctactaa actgcgcgaa ctgggcgatg ttattttggg cgagagtttg caggtgagtg
     4501 ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc tatattggct aacggtagtt
     4561 aacgttgttg cgacctgcgc acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc
     4621 agaattttca ccgcattatt gagctgcttt tccagcgttg gtgcattttt ctggatctgc
     4681 tccagcccca gattcatcac catttgctgc acccgcagag cgctaaggcg cagttcattc
     4741 atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact ttcagcagcc
     4801 tgacgctgat cttgttcgat caaatcgtaa atcccggcct gggtcgctcc agcggaagtt
     4861 gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc agcgactatc
     4921 tgctgactga gttgccgttg ttgctggcgt agttgcagac gccgccccac cagttcccct
     4981 tgctgacgta atgaacggga gatctcctgc tcctgttgtt caatagcggt ggtatcaaaa
     5041 ccttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt
     5101 cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa
     5161 agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc
     5221 tgagtgttgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta
     5281 ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt
     5341 aaattcacgg tcggtgcact taaggtgaaa aaggttgagt cgcaaagcgg aatgcatcta
     5401 gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc
     5461 cttttcatgt tatcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat
     5521 tacacggtgc aagcctctac gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt
     5581 tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca ggaggcagct
     5641 cgccgctatg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc
     5701 cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt
     5761 agcactacgt catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg
     5821 gtaaatgctc ttgatgatcc cctggtgaaa agccgcgttg gtgtaccctg gtttcagatg
     5881 ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg
     5941 ttgatgcccg gacccgataa tgccgggggc agtaaagaga tggtcgaggg ttttcgcgca
     6001 gccattgccg gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa
     6061 atccagcgta acctgttgca ggagatgctg gaacgccacc cagaaatcga catcgttgcc
     6121 ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt
     6181 accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg
     6241 attatggctg ccagcgatca aatggtctgg cagggggaac tggcggttga gcaggccatc
     6301 aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg
     6361 ccgaaaaatg ccgaccgtga acatattcgc cgctcgctgt caccaggggg atttcgtccg
     6421 gtctattttt atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc
     6481 agtaaatccg cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg
     6541 tcagggtttt ccacccgacg cgcagaaagc atacgtagca gacgttcacg gctgagaatt
     6601 tcgcccggat tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc
     6661 ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc ggcaaagcga
     6721 tagcagttgt cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga
     6781 tttttcaccc gtactaccag ttcacgcagt tccagcggtt tggtgacgta atcgtctgcg
     6841 cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc
     6901 cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag
     6961 ttgatatcca gcagaattaa atctaccggc tgattctgca taatttcccg cagcccggca
     7021 ccgctcgctg taacggaaac ggtatacccc tcctgagtga agtaagattg taatcgcgcc
     7081 tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca
     7141 ttctgttcat atctgttcat attctgccgt aagccgttca tcctgaccag tgccgctgtt
     7201 catatttgct cattaagatc gcttcactaa accataattt tacaggggct attatgcgga
     7261 aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcaa
     7321 ttgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca
     7381 gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac
     7441 agtcggtgca tttccagaac gcctccggcg tgcgagctga atgccatgac tgtcatatcc
     7501 cgccggatat tccaggcatg gtgaagcgca aactggaagc gagcaatgat atctaccaga
     7561 cctttattgc tcactccatt gatacacctg aaaaattcga agccaaacgc acggaacttg
     7621 ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgccgc tcctgccata
     7681 actacgatgc gatggatcat gcgaagcagc atcctgaagc ggcacgtcag atgaaggtgg
     7741 cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg
     7801 atatgagtag cggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgacagtg
     7861 gtgacacgct gtactctatt gatattaagc caatttatgc ggcgaaaggc gataaagaag
     7921 cctctggttc tctgctgcct gcttcggaag tgaaagtcct taaacgtgac ggcgactggc
     7981 tgcaaattga aattaccggc tggacggaaa gcgccggacg tcagcgtgta ctcacccaat
     8041 tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtaaaaa
     8101 cgctggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggccactg
     8161 cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggct tatgcggatt
     8221 cgttgtacaa cggcacctgt aaccagtgcc acggcgcacc ggaaatcgcc cactttgacg
     8281 ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagtctc gataaacgtg
     8341 aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaggctc
     8401 acggcgataa gaaggaagaa aaataatgaa caataacgat ctctttcagg catcacgtcg
     8461 gcgttttctg gcacaactcg gcggcttaac cgtcgccggg atgctggggc cgtcattgtt
     8521 aacgccgcgc cgtgcgactg cggcgcaagc ggcgactgac gctgtcatct cgaaagaggg
     8581 cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg gtcgctttgt
     8641 ggcggcaaaa ccgttcgaac tggataaata tccgtcgaaa atgattgccg gattgccgga
     8701 tcacgtacac aacgcggcgc gtattcgtta tccgatggta cgcgtggact ggctgcgtaa
     8761 gcgccatctc agcgatacct cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga
     8821 tgaagccctc gacatgttct atgaagaact ggaacgcgtg cagaaaactc acgggccgag
     8881 tgccttgctg accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat
     8941 gctggcgaaa gctattgcct tgcatggtaa tagcgttggt acgggcggag attactctac
     9001 cggtgctgcg caggtgatcc tgccgcgcgt ggttggctca atggaagtgt atgaacagca
     9061 aacctcctgg ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctctgattt
     9121 gctgaaaaac cagcaagcga actggtggtg cccggatcac gatgtttatg aatattacgc
     9181 gcagctaaaa gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg atccggttgt
     9241 cacatccacc catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca
     9301 aactgatgtg ccgctgcaac tggcgctggc gtatacgctg tacagtgaaa acctgtacga
     9361 caaaaacttc ctcgctaact actgtgtggg ttttgagcag ttcctgccgt acctgctggg
     9421 tgagaaagac ggtcagccga aagatgccgc atgggctgaa aaactgaccg gcattgatgc
     9481 cgaaaccatt cgtgggctgg cgcggcagat ggcggcgaac agaacgcaaa ttattgctgg
     9541 ctggtgcgtg cagcgtatgc agcacggtga acagtgggcg tggatgattg tggttctggc
     9601 ggcgatgctg gggcaaattg gcctgccagg tggtggcttt ggttttggct ggcactataa
     9661 cggcgcaggc acgccggggc gtaaaggcgt tattctgagt ggtttctccg gctctacgtc
     9721 gattccgcct gttcacgaca acagtgatta caaaggctac agcagcacca ttccgattgc
     9781 ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt
     9841 aaaactgccg ccgctgaaaa tgtgtatttt tgccggaact aacccattcc atcgccatca
     9901 gcagatcaac cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa
     9961 ccagtggacc tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga
    10021 gcgtaacgat ctcgaccagt acggcaacca ctccaaccgt ggcattatcg ccatgaaaca
    10081 ggtggtgccg ccgcagttcg aggcgcgcaa cgactttgat attttccgcg agctgtgccg
    10141 tcgctttaat cgcgaagaag cctttaccga agggctggac gaaatgggct ggctgaaacg
    10201 catctggcag gaaggtgtac aacaaggcaa aggacgcggc gttcatctgc cagcgtttga
    10261 tgacttctgg aataacaaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca
    10321 ccaggcattc cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga
    10381 gatctactcg aaaaccatcg ccgatatgaa ctacgacgat tgtcaggggc atccgatgtg
    10441 gtttgagaaa atcgaacgct cccacggtgg gcccggctcg cagaagtatc cgttgcatct
    10501 gcaatctgtg catccggatt tccgacttca ctcgcagtta tgtgagtcgg aaactctgcg
    10561 tcagcaatat acggtagcgg gtaaagagcc agtgttcatt aacccgcagg atgccagcgc
    10621 gcgcggtatt cgtaacggtg atgtggtacg cgtctttaac gctcgcggtc aggtgttggc
    10681 aggggcagta gtttctgacc gctatgcacc cggcgtggca cgaattcacg aaggggcatg
    10741 gtacgatcca gataaaggcg gcgagcctgg tgcgctgtgc aaatacggta accccaacgt
    10801 gctgaccatc gacatcggta cttcgcagct ggcgcaggcg accagtgcgc acactacgct
    10861 ggtggaaatt gagaagtaca atggagcagt ggagcaggtg acggcgttta acggccctgt
    10921 ggagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat gaccacgctg
    10981 acagcacaac agattgcctg tgtttacgcc tggctggcgc agttgttctc ccgtgaactg
    11041 gacgatgaac aactgacgca aatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg
    11101 aaaagcgaac cgccgctggc tgcggcggtg aatgagctgg aaaactgtat tgccacgctg
    11161 acagtacgtg acgatgcccg tctggaactg gccgctgact tttgcggcct gtttctgatg
    11221 accgacaaac aagcggcgct gccgtatgca tcggcctaca aacaggacga gcaagagatt
    11281 aaacgcttgt tagttgaggc agggatggaa accagcggca atttcaacga accggcagat
    11341 catctggcga tctatctcga attgctcagc catctgcatt tttcgctggg agaggggacc
    11401 gttcctgcgc gaagaatcga cagtttgcgg caaaaaacac tgacggcgct gtggcaatgg
    11461 ttaccagagt ttgttgtgcg ttgtcgtcag tatgacagct ttggttttta cgcggcacta
    11521 agccagttat tgctggtgtt agtggagagc gaccaccaaa acagataacg tcgtttgtgc
    11581 gcctgaaaag acgcgtttag cgtcgcatca ggcattatgg ggcagttgcc ggatgcgtca
    11641 tcacatccgg caaacacaca tcacggatga gctacaaacc gggaaagtcg ctggcgcagc
    11701 aggcggtttt cctgcttcag gtgcgcaata tcatccatta acgtcagcgc caccgcgatc
    11761 ccaggccagt ccagagccag ttcatgacgc aggcgtactg cgcgttgcac cacgatggct
    11821 gcatggtcgt caaataccca ggttgtttcc tggatctcac gcggttcaac cacccccaaa
    11881 ccgacaattt cattcaactc ctcttcagag atgccggtat gcaggcaaaa ttcggtaata
    11941 gtaaaagtca ccgtaacatt agccattatg ctttccccca atctttacgt ggatcaaaag
    12001 acgactgggc gtctgccagt tgctgccaca gcgcggcagt gttttcatcc ggtttcggcg
    12061 gcatcacgat tttcagtacc gcatacagat cgccggtctg ttttttgctc accagacctt
    12121 tgcctttaac gcgcaatcgt tgcccggcct ggctgcctgg cgggatagtc agcaaaatgc
    12181 tttctttcag tgttggaacg gtgactttag cacccagcgc cgcttcccac gggctaaccg
    12241 gcaccacaat ttccagatcc tggccgacaa tatcgaacag cggatgtggc gcaatatgaa
    12301 tcaccagcca caaatcgcca tttggaccac cgttttcgcc cggcgtcccc tggcctttca
    12361 gacggatgcg ttgaccattg ccgacgcccg ccgggatctt cacattcagc gttttcggga
    12421 tttcctgttc gatcatgcca aaggcgttat aaaccggcag gttatagctg atggtacgct
    12481 tatgctcagt aagcgtttct tcgaggaata ccgccacttc gatttcaata tcgtggccgc
    12541 gtgcggcggg gcgttgacgg ctctggcggg catgctgacc gaaaattgac gagaagatat
    12601 cgtcaaaatc ttcggcatta aaactctgac cgtcgccatg atggaactga cggttaaatt
    12661 gcggatcgtt gcgatgttgc cacatctgat catactcagc gcgacgttgt tcatcactta
    12721 acacttccca ggcttcagcg acctctttga agcgggcttc ggcatccggt tctttgctga
    12781 catcaggatg gtatttacgg gccagtcgac gataggcggt cttgattgtc ttgagatcgt
    12841 ccgtcggttt cacgcccatg atggcgtaat aatcctttaa ttccatagcg ttatctcgcg
    12901 taaatcaaca caaattgaag gaacccctgt aaggtaactc ctataagtgt agggtaatcc
    12961 tcaaaatttc atatgccaac acagaatatg ttattgaaat catcgcggag aggaggtcgc
    13021 ctcaaggtgg ggtgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata
    13081 gtggaaccag gcactttaag tttaactggg gcgtcattat ttattaaatt ttataggcgc
    13141 tatatatgag tagtaatata catggaatta gttgcactgc aaataattat ttgaaacaag
    13201 cctggaacaa tataaaaaat gagcacgaaa aaaatcaaaa atattcaatc acgctttttg
    13261 aaaacacact ggtgtgtttt atgcggttgt ataaagaaat cagacgtcaa aaagctgaag
    13321 actacattcc atgcctggaa tgcgattcac tagaaaaaga atttgaggaa atgcagaatg
    13381 ataatgattt atcattattt ttgagaacat tgcgtactaa tgatacagaa acttattcag
    13441 gggt
//
LOCUS       DAZFFI010000119        13397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFFI010000119 DAZFFI010000000
VERSION     DAZFFI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.119"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..113)
                     /locus_tag="ACHJ12_004049"
                     /pseudo
     CDS             complement(1..113)
                     /locus_tag="ACHJ12_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
     gene            complement(169..615)
                     /locus_tag="ACHJ12_004050"
     CDS             complement(169..615)
                     /locus_tag="ACHJ12_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991760.1"
                     /translation="MSEGTRVNDFAYVKQALLAVFHRMNTRPLTRLCEKDDIQRGIAD
                     IQWMLHHHYYPSPGQVGFIIFLLRDEKFRVVREDGRQSFLAVEIQSLIDVLKDIRKYL
                     QFVTRYDCDGCIIRLHASAERKYLWIFLECVIVFILCAVLFFLIIC"
     gene            complement(1344..1688)
                     /locus_tag="ACHJ12_004051"
     CDS             complement(1344..1688)
                     /locus_tag="ACHJ12_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991761.1"
                     /translation="MKAVEVYGRHSDVVMSLERQTDLLEAAGNVLKQRWFYRPWGSSK
                     ASVMLTGILVIPVFLVLSGLLSAGYPGLLCITVSGCYFSGIAAFSLRAKDPVGLCWSV
                     FSFILLYLLLKK"
     gene            complement(1900..2892)
                     /gene="traU"
                     /locus_tag="ACHJ12_004052"
     CDS             complement(1900..2892)
                     /gene="traU"
                     /locus_tag="ACHJ12_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO4991762.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAVDAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(2889..3521)
                     /gene="traW"
                     /locus_tag="ACHJ12_004053"
     CDS             complement(2889..3521)
                     /gene="traW"
                     /locus_tag="ACHJ12_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO4991763.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEADMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(3518..3904)
                     /gene="trbI"
                     /locus_tag="ACHJ12_004054"
     CDS             complement(3518..3904)
                     /gene="trbI"
                     /locus_tag="ACHJ12_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO4991764.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(3901..6531)
                     /gene="traC"
                     /locus_tag="ACHJ12_004055"
     CDS             complement(3901..6531)
                     /gene="traC"
                     /locus_tag="ACHJ12_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO4991765.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGERIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGLNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(6662..7009)
                     /locus_tag="ACHJ12_004056"
     CDS             complement(6662..7009)
                     /locus_tag="ACHJ12_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991766.1"
                     /translation="MKSIYLKYFLSFVFVGVMAMIVCIPFYIIYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTFGKANKIASECRKRNEMEEARRVRENELNKIREKQ
                     LQALSDADSAKER"
     gene            complement(7037..7255)
                     /locus_tag="ACHJ12_004057"
     CDS             complement(7037..7255)
                     /locus_tag="ACHJ12_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991767.1"
                     /translation="MHTFIFLVLMKNVGGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            complement(7335..7808)
                     /locus_tag="ACHJ12_004058"
     CDS             complement(7335..7808)
                     /locus_tag="ACHJ12_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991768.1"
                     /translation="MHKSENEKIDSILDEHQQIYRRHNRIDNILAFFACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(7801..8022)
                     /gene="traR"
                     /locus_tag="ACHJ12_004059"
     CDS             complement(7801..8022)
                     /gene="traR"
                     /locus_tag="ACHJ12_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO4991769.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(8157..8672)
                     /gene="traV"
                     /locus_tag="ACHJ12_004060"
     CDS             complement(8157..8672)
                     /gene="traV"
                     /locus_tag="ACHJ12_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO4991770.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(8669..8920)
                     /gene="trbG"
                     /locus_tag="ACHJ12_004061"
     CDS             complement(8669..8920)
                     /gene="trbG"
                     /locus_tag="ACHJ12_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO4991771.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            complement(8932..9126)
                     /locus_tag="ACHJ12_004062"
     CDS             complement(8932..9126)
                     /locus_tag="ACHJ12_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO4991772.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            complement(9113..9697)
                     /gene="traP"
                     /locus_tag="ACHJ12_004063"
     CDS             complement(9113..9697)
                     /gene="traP"
                     /locus_tag="ACHJ12_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO4991773.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            complement(9687..11117)
                     /gene="traB"
                     /locus_tag="ACHJ12_004064"
     CDS             complement(9687..11117)
                     /gene="traB"
                     /locus_tag="ACHJ12_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO4991774.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            complement(11117..11845)
                     /gene="traK"
                     /locus_tag="ACHJ12_004065"
     CDS             complement(11117..11845)
                     /gene="traK"
                     /locus_tag="ACHJ12_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO4991775.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            complement(11832..12398)
                     /gene="traE"
                     /locus_tag="ACHJ12_004066"
     CDS             complement(11832..12398)
                     /gene="traE"
                     /locus_tag="ACHJ12_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO4991776.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(12420..12731)
                     /gene="traL"
                     /locus_tag="ACHJ12_004067"
     CDS             complement(12420..12731)
                     /gene="traL"
                     /locus_tag="ACHJ12_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO4991777.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(12746..13099)
                     /gene="traA"
                     /locus_tag="ACHJ12_004068"
     CDS             complement(12746..13099)
                     /gene="traA"
                     /locus_tag="ACHJ12_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO4991778.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            complement(13134..13361)
                     /gene="traY"
                     /locus_tag="ACHJ12_004069"
     CDS             complement(13134..13361)
                     /gene="traY"
                     /locus_tag="ACHJ12_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO4991779.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
BASE COUNT         3287 a         3683 c         3036 g         3391 t
ORIGIN      
        1 cagttacgtt tacgccacat caggtaaccg aagtttttct ttgtgtcagg cggttttttt
       61 tccgtctccc ggcgcatcac actgcgcccg aacggatggc actgtccgct gtccgctgtc
      121 cgctgtccgc tgtccgctgt ccgggtacat gttcaccacc aggtgttttt aacagataat
      181 cagaaaaaac agcacagcac acaaaataaa cacaattaca cactcaagga aaatccacag
      241 gtatttacgt tcagcactgg catgcagcct gataatacaa ccatcacagt cataccgtgt
      301 cacaaactgc aggtatttcc gtatatcttt cagaacatca attaacgact gaatttcaac
      361 tgcaaggaat gactgacgcc catcttcccg cactacccgg aacttctcat cccgcaataa
      421 aaatattata aaaccaacct gtccgggtga tggataatag tgatgatgta acatccattg
      481 aatatctgca attccacgct gaatatcatc tttttcacat agcctggtga gcggccgggt
      541 attcatccga tgaaacaccg ccaaaagggc ctgtttgaca taagcaaaat catttactct
      601 tgtcccttca ctcatttaat atcaccctta aacatctgtt catgtatcag tttcggagtg
      661 caaatggtca taatactgat attcttatcg ctgttgttaa cggtcctgct tcgttttcgc
      721 ttcataaata acagtctctt tttcagagtt ctgagaagac cattcccggc tggctttatt
      781 ttgtttttac tgtcatccat catacatctg atgatacaaa aagattattc acctttcagc
      841 ggaaacatat actctgtaat tatcaggtca gggatatctg taattacact atggtttttt
      901 ctgttctttt tctcatcgtc catgctcaga acgcctgtta cgggtcttta tcttaccccc
      961 tttatatcca gcctgagaat cgatatacat ccacaatata ttaccagacc atctgtcaaa
     1021 gtatcctcaa gaatcaacgc agacaagctt gtcggaaata ttgaacattt catattatgc
     1081 tatccgtctt ataacacgat cacattacag tctcacatcc tgcgccacgg aatccgaaac
     1141 agaatagcta ataacctgaa aaaaaaaggt atactatata aagaaaaggc aaggaagacg
     1201 ccactctgtg aaactattgt cctgaatctg aggtatgggg gaaaaacccg ctacagactg
     1261 ttctcctgca ggaggcatcc caatggattc agagtacatc gcaatggcgg agcattcatt
     1321 attcagacgc agaataatta atctcatttt ttcaggagca gatatagcag aataaaactg
     1381 aacacagacc agcaaagccc gactggatct ttagcccgca aagaaaaagc ggcaatcccg
     1441 gaaaaataac agccggatac ggtaatacat aataacccgg gataccctgc actcaacaat
     1501 ccggataaaa ccagaaatac ggggatcacc agtatacctg tcagcatcac agatgctttt
     1561 gatgatcccc acggacggta aaaccagcgt tgttttaata cgttgcctgc agcctcaagt
     1621 aaatcagtct gtctttccag agacatcacc acatcagaat gtcgcccata aacttcaact
     1681 gccttcatcc tggccatcca taactgtgca cacaattcat aaatttgtgc aggtgttacc
     1741 cgttttcctc cggacaaacg agcagcgacg tgatccgtct cttctgcaat ctccctcaga
     1801 gagataaact gtcgtaatgg ctccgtgttc agtgaaagac tgacctgtct gaggattgat
     1861 acaagttgca ctttactgtt gcgcatcaca ttttcccgat tacaggaaca cacagttacg
     1921 tttacgccac atcaggtaac cgaagttttt ctttgtgttg ggcggatttt ttcccgtctc
     1981 ccagcgcatc acactgcgtc cgaacggatg gcactgtccg ctgtccgggt acatattcac
     2041 catctgataa cgccagcgct ctttgggaag tatgggcgac ggatattcat aacagacagc
     2101 cacatccacg ccgatgctgt tcattatcat tccctgacgg tggagcttaa acgccatccg
     2161 ttcactgacc agcagggatg actgcaacgg actggattca ttgctcaccc agccattaaa
     2221 cggatacata ctgccctgcg aaccggcaca ccagaacagg atatccagcg gcttgttaaa
     2281 ggcactggca atggcatcca ccgcacaggc tccctgtgct atcggatttg caaagatgac
     2341 cgcttccggg ttgagaatgg tggtcagact gctgtctgtc caggtagggt caatttctga
     2401 gatgtacccg atatccatat cgccgcccat cagacatcct gctgaggtga taatactcag
     2461 ccagtatgtc agcgggtatt tgtaccagtg aacatggtaa aatgccccgt tcacctgctt
     2521 gtcgtctttt ttggccgttc cctgcccggt tttccccagc tccgggattg ttattccaag
     2581 atttaccata catcccggac tgcgggtcac atccgtcagt gccatcggct cccagtaacc
     2641 aatagccagc ccgatacgtt taaatacagg cggtggcgca ggacagaact ggatcggcat
     2701 tgacgggttt attgtatccg gcaccttccc ctgactcact tttgtgctgc ccagcgacag
     2761 tggaaaaatg cagttccagc agatatccgt gatcgggtta acaaaccgcc cttcacaggc
     2821 agcgtccgct gaggctgcgg gaacatggcc agtcagcagc aaaaccacac acaaccacat
     2881 acaccgtttc atttccctcc ctccccgacc ggaataaact ccaccttcag gaaacgatcg
     2941 ccactgacag cactcacccg tgccggcacc tgatcaatgc cgagacgctg gcagatcacg
     3001 ccgttctgat caaagtaaac acgactgtcc agagccttct gcatctccgg gatactgccc
     3061 tgcaccagga taattttgct ctccagtgtg ggaggcgtct ggcgcttcat ccattcaatc
     3121 tgctcaggat catcaccgtt gatgaaatac agtgtctggt taaaaggcac atactgcagc
     3181 gggttcatca cctccccctg gcgggcgaag atacgacctt cgttatcccg gatatccgca
     3241 gccagtctga cagacgggtc aaacaggcgg ctgctgtatt tctccgtccg accgataccg
     3301 ggcacggcag ggggccgcag gctgttgcgg atcacccgct ccttaaacgc atccattttc
     3361 cggcccatct ctccggactg ctccagagct gtcagacgct gcataatcac cgtcagcatg
     3421 tccgcctcct ttaccggcca caaatccccc caggtgccaa gatcagctgc agccacactc
     3481 tgcccccata ccagcagggc aatcagcccc cggcatctca tggttctgcc ctcattcgcc
     3541 gggcaatatc ctgctggatt tcacgggtga tatccggtgc cccctgtacc acggcaggcg
     3601 acaccagaat gacagcgtgg tgcttctgct gccacgtctg cagactggcc tccagtgccg
     3661 cgttaaaacg gtcagccagc gccttcgact gcgcttctga cagttttttc tggctggcgc
     3721 tgtcaaaaaa cgcatccact gtctgtttca tgttgaaggc gacggttacc ggcgtgttca
     3781 gtctgacaat gccgtaactg acggctgcgt tcagtaacac cattgccagg catcccggca
     3841 ccgtccacca ccagtggctg cgtcgacggg ctgtcacatc agtcgttttt tgcgttgtgg
     3901 tcatgcaaca ctcctgtatt tctcatgttc ttccagccat gcttccagcg aagccatctc
     3961 cggccctgac ttcttccacg ccagctgcca caccgcctca tgaatactca gcccctcttt
     4021 acgtttctgc tgcacaaact caaaatccgg gccgtcagag ctgtacatgg cacggcttag
     4081 cgggtccaca aacagacggt gccaggagga gtggttttcc acctgcagca ggaaggaact
     4141 gaaccactgg tctttggccg caccaaactt accaatcatg tcgcgctgca gtggcaggaa
     4201 ctggtccgga tacagctggt tatatttcgc gaactccttc gcactctgtt tgaggataat
     4261 tttgtaggag gagttacccc atgccgcacg ggccgcactg gaggccttgt cagagtcaaa
     4321 gtcgacgatg ttctgtgtga tggtgatata ggcaccagta tgacggcggg cggtacggta
     4381 acctttctca ataaactcac cgaccttgtg atttttgaag tccagcagac gccacccctc
     4441 atcaatgacg ttcagtttct tgaggttacg cggcgtgcgg tacatccggt tctcgatgta
     4501 aatgataagg ctgaacatca ccgcaaccag cagggacgga cggtcttcca gtccacccag
     4561 ctccagcacc accatttttg cgtcatcccg cagggacggt tcatcagagt taaaatactg
     4621 gccgtaagta ccgttggctg tgtactggtc cagcagcact atcatttcat ccagacggct
     4681 gcggatggtc ggggactcgg catactggtc gttatcccgg gcgtttttca ggaaatccac
     4741 cacgtcatca atacgcgccc ggttctgtct ggtaagccag gaggccctga ccgcctgcag
     4801 cagcagacct tcatgcactt catccaggtt accgttgggg ctggccatca ccgacagctg
     4861 gtcacggaca cgctccgctg actggtcaat atcggtgatg ttcgcaaacg ggttaaagcg
     4921 cagggtttca ccgtccagat atacgccccc catgttttca cacagggact tgtagccatc
     4981 ccccatgtcg aacaccacag caaaaccccc ggagtccagc acactgcgga taagtggctg
     5041 tatcagcccg gttttaccgg ccccggaagt gccacagacc gccatgttgt agttggtgtt
     5101 attcatcccc cggaagaaaa tatcgataaa cgccagctgg ttacggtagg tgggtgccag
     5161 cagacctgcc ggcgtcaggg ggttatccgc cactaacggc atcaggttgg ccacattaaa
     5221 gctctctgcg cgctgaacca ctccggcctc tttcagttgt ttaaacaacc ctttcccggc
     5281 cataaagggc aggcaggtca ggaaattgcg catgtggtta aagcgcggtg aaatcagttc
     5341 aaaaccgttt ttacggaagc tgttcaggat atcctgctcc acttccagtg ccgtttcatt
     5401 attgtccttg cagaatgctg tgatgttgag gaagtaggac acgacagaag actgaccgga
     5461 gcccagccgc tgacgcagtt ccccccactc cttcgcttct ttctccacgg acggaaacca
     5521 tttggcatag gaggtcttcg actttttctc caggtccatg tacttcaggt tggcttcgct
     5581 gtgggttttc acctggtctt ccaccaccag ggtcagcgtc agaatgaacg ggcaggagat
     5641 ggacagttcc gggttcagca ggttgctgta gttgtcggcc atgttccaca ggaaggcgat
     5701 ttccgggtta cgggccagat ggaaattcag gatgcgggca gtgctgttcc tgccgttctc
     5761 acgcagtccc agcgtcaggt aatctgcccg gaccttcagg tcaaaactgt cctccacgca
     5821 ctgataattc aaatcagaat acgggtccag ctgacgtctt ttcgggtaca gggagtccgg
     5881 gttatggtta atcatctccc cgacaatatc gatgaaagcc tgtgcatcca ccgtctgcgt
     5941 ggtgatatat gctccctgca gagacgcccg gatgattttc accaggtttt ccatttccag
     6001 aatgtcggcc cggctttttt tcttcgaggg agaacagtac gagataaaca cccggtaatg
     6061 gcgcagggtc agaggcaggt tcaacccttc cggcagcgga aacagtgttt ctgcggcttt
     6121 catgtaatag gcccgggtaa tggcgttaaa ccgttctgcc tgttcacctg accaggagaa
     6181 ctcacgcagc ccgtactcaa tccgctcgcc aaccagctgg ctggacatca gatggataca
     6241 cagcggaata ccgcgcggca gtttggtgcg cagcatatgg tcgagagcct ccacaatggt
     6301 ctcattcgca ccattgatgg gaatggcttc cagcataaag cccattgtgg agtcattcat
     6361 gaacagaccg gattcctggt tgtaatcacg gtatggcagc agacggctga actgcgggaa
     6421 gttcatctca ccgaggatct gattagcctg agcagattca tccggtaatt taagtgcggt
     6481 cagcagtgaa ttgaccgcct gagtgacgac ctcaagtggg ttattactca tatgagtatt
     6541 ccttagtacg tttaaaaaag gaaaatatca gtctgtggcg tggatatatc acgcagtgcc
     6601 atttctgaaa ccacagcaca aataagagat ggaggctttt aacaggaaac caggattaca
     6661 attaacgttc ctttgctgaa tcagcatcac ttaatgcctg aagttgtttt tcgcgtatct
     6721 tattgagctc gttttctctt acccgtcgcg cctcttccat ttcatttctt tttctgcatt
     6781 ctgatgcgat tttatttgcc ttaccaaatg ttaaaggttc agactgataa ttaagtgtct
     6841 cctgaaatgc ttctgctgca cagggagttt cctgtaaaat ctctgtcagt tgttctggtg
     6901 ttgcaggttg ttgcgcaagg taaatgatgt aaaaaggaat acatacaatc atagccataa
     6961 caccaacaaa tacaaaagac agaaaatatt ttaaatatat cgacttcatt tcattttcct
     7021 tcgaagcagt aagcactcat agcccaccac cattcagtgt ttccatcacg ttattcaaag
     7081 gttcattagt tttaacttca atacactccc ctgtatttcc aaatcccctg cctccatgtg
     7141 cttcgattgt ccgaagaacc tgcacgcata cgtccgtatt tggcatgggg gtaacattcc
     7201 agcttccgcc accgccaaca tttttcatca gcacaagaaa aataaaggta tgcattcttt
     7261 attacctcca gtgaaatatt aatcactgaa tactccttca gttaacttat gaggttataa
     7321 aacacagcag attttcattc ggatttattc ccttcagtaa acttccggat ggtatcctgt
     7381 tcacgctgac gggtggcaga ctcctgcatt gcatccactt tctcctgcca gagacgccgg
     7441 atatcctttt catcccgacc agtcagtttt ttaccggaca gcagacggtt aagcagttcc
     7501 tgtctcgcat ccggtacgtc ttcagtcaga cgcataagct gaagcaggct ctgacggctg
     7561 aggaccatga gtgattccgg caggagagtg aagaagaaaa taaaaaagca taccgcacca
     7621 cagcaggcaa ggaccgcaaa caggattttc agatatatac catcaacgcc gaacgcagca
     7681 ataatacagg gaatccatgc aatacacgca aaaaacgcca ggatgttatc tattcggtta
     7741 tgtctgcgat atatctgttg atgttcatcc agaatagaat ctattttttc attttctgat
     7801 ttatgcatag tgtttcctct gcctttcctg atacgcctga cattcaacgc acaacgtcac
     7861 accgggaaat attttccgcc gggcttccgg aataggattt ccgcatgctt cacagagata
     7921 aacaggaata ccatgaacat ttattttctg gcgtatccgg tttattcctg tcatggtcag
     7981 ttgttctgtt actgaatatg cttcatcggc ttcatcactc acggaatatt cctcccgaca
     8041 tgttctgaca cgctaccgcc catccctttt cacgcacagg gcactcgttt tacaggcaga
     8101 ttttgaatac agaaagcccg ccggcattgc cggctgatat acaacttcag ggcctgttaa
     8161 ttaatacgtg gttttcccca cgcagacggt ttaataacaa aaaacacact ggatggctga
     8221 tgataaacat cctgattatc aatataaggc gctatccata atgctgccgt ctgctcaccc
     8281 gttcttagcg gacggggagg tattaccggt gcaactgaac tgaccggaac aaccgttttc
     8341 acttcccggg cagcggtaaa cagcggacgt ggtgccagca gtttctgctc cggaagtgcc
     8401 gtcaccgcag gtctgctgcc ggacggagag gtcgcggtga cggtctgcac cggcattgtc
     8461 cggaagttcc cttcagccag gcgctgcagt gatgccgcaa ccggctttgc ttctgaggac
     8521 tgctccagtt ttttggcctt ctcattggcc tgctccatcg tcatacaggt atcggatgtg
     8581 gtggcatcac actcaaagtc ggagtttgtt ccggcacagc cggaaagtaa taacgtcccc
     8641 agcagcggaa taaataaaga aatctgtttc attgtccctc acctttctca tcgcagttca
     8701 caaaatcatt cttcaaatcc ctgaccgtac aggtttttac aatgtcagga ttttcaaaaa
     8761 ccgtattccg tgatgattta taaacatatg ccacaatgcg tttcccaccc gcatcaggtt
     8821 ctggtgcact gacttcacat aacggaggtg ttattccgga ctcatacagc accggataat
     8881 ttattttttt gacgctgcca tcagaaacca gcttattcat cgaagggttc cttatatttc
     8941 tgaattcttc atcacaccga cagcagtcat agtaaaattg tccggcaatg actcagaacc
     9001 gaatttctcc ggtgcaggaa gtactcctgt ggtgcagtta ctgagaacag catgcataaa
     9061 atcatctgat atgcgtttat ccggtacaga gagggccgta ataacatgaa tattacgcat
     9121 gttcattttt atctccgttg tttctgtttg ttctgttatc agggaaataa gcatggcatt
     9181 tcctgatttt tcccggcagg gaagcgtctt ccgtataaac ttccttcaga tgttcatctg
     9241 cggttaccac ccggatacat ttcatttttc cgggtcgcac cagatatttc ccggtgattg
     9301 ctgccagcga gacatacatc agtgccagtg ttgcccataa tgcggtgacc agttgcatca
     9361 gtgaccggtc agtctccctg acataatctg cagcatcagc agcacggtaa cggcagattt
     9421 tctcttctgc tcctggaaga tctggcagag aaggcaaaca ttctcttaca ggaaactgcc
     9481 cggcaggggc tgtttgtctt atgaagtcaa tttcctgaac cagtaatttc cctggtgtgg
     9541 tgttgtcctt ccataaaaca aataacgcca tcagggccat tgtggccagt ggcagaatga
     9601 cggtgtattt cagacaccag aacagtccac gcagaacccg ggcgacaaca taccgggcgg
     9661 tatggcacgc ctgactggac gacgtattat ttgccatcgt tgcccccttc ctgaccaccg
     9721 gccataaact gctgcggatt caggtcgccc agttgcttca gcatctggtc ggtattcaga
     9781 ccattcaggt gactctctgc ggatggcgga ttcggcacgg gacttcccgg gttactctct
     9841 tccacctgcc cctgagcctg ctccagcgcc atctcttcca cggttttcag ctggaaaccg
     9901 tcctggaaca ccaccgtcac ttcattaccc gccccgatgg gtatcaccgg gtgatactgc
     9961 tcagcgcgtt tgatgtagta gtcactgagt gtctgtgccg ctttcgatgc accgccaccg
    10021 atacccattt tcaggacatc gccggccccg taagcggcag tggcacccag cccgacagcc
    10081 ggctgggaag cgcgctccat tccctgaccg ataccgtcaa caaatcccgc gccccatgcc
    10141 cagccgagga ttttgccgtt acgcatcacc acttcaccct tgataccgtt tttaccccgg
    10201 aagctgacat gccctttaac cggcatatcg atggttttac cctccttcag gcagctgata
    10261 ttgcgggtac gcacgatggc acgctcactg gacacatccc cccaggcttc cagcccaaca
    10321 aagcatcccg ttgcgtcata ggttttgctg ttcggcattt ccaccagacc ggtgatacgc
    10381 agctgcatcg gcaccgtgga ttcattaccg gtgacagaag cattggcatc cgccccttca
    10441 atcagaatgg ctttcgcaaa actgcctgac ggaatgtacg gcagggacgg agtttgcttt
    10501 gtgccagcat catagctgaa cgttttacgc tgtatccggt tcggcaccgg cacagactgg
    10561 tacgtcacct gtggcggcgg cgtggctccg ttgcccggat agaacgcggt gggcggtggc
    10621 actgcacctg ccccctgcgg tggaaaagca acgggcgcat taccgggctg aggctccccc
    10681 tccggaccgg gaggggatgc cggcatctgt ggcacaggct ctccggtcac tgtaacggga
    10741 ttcgcgccca gggctttcac ctgctctgcc agcgcagcat tctcctgccc cagcttttca
    10801 atgcgacgct ggtcatcacc acgctgtttg ttcagaacat ccatctcacg acggatttcc
    10861 tcatactgct tctgcatctg tgccgccgtc acctgcatct ctgtggtggc atgctgacgc
    10921 accttgtcat caaaggtcgt atccacaaca ccggtcatat ccggcacagg ctcctgccct
    10981 gcaacggcat cagtgttccc ggacatatcc atatcagaca gataaagcgc accaccaatt
    11041 gctgataccg atccgacaat cacaatcccc agccacagga actgcttgcg tttcacaatg
    11101 gtgttgatac tggccatcag ttgccctccc cgtcacgaat gacgtacaca tccatacggg
    11161 cagcgaccag taactggctg acaggctggc tgaacatcac cgcccgggtt cccggctgcc
    11221 agaagtcact ttcccggata ttcagcgcag acagcgtttt attttccacg gtaaagcgca
    11281 ccatcttcag gtgattcccc gtccatacgg cttcagcttt tgcggacatt ccggcagggg
    11341 cctgcagggt ttcctttgtc acggggatct gataccagcc ttctggcagt tgtccggcac
    11401 gaaccgcctg actgatggaa accagcaggg attcgtaagg cgtggatgtt tcccatgcac
    11461 cggcagtttc tcctgttccg cgcaaatcgc tgaccagctg aatggtacgc ccggctcctt
    11521 cacggggaac ggcctgaatg gaaagattaa gaccacgttc tgtttccaga atgaacgtaa
    11581 aaggcttttt gttgacggtg gccaccacca caccaccgga cgcagtctgc tcattattgg
    11641 tcagcgcacc atccagactg ttcacggcga taatccggtc accggggacg gtaaacagat
    11701 tcgggctggt attactgata gcaatacttg cctgaccgcc attgaccatc ggaaccactg
    11761 taggtgccag tgtgccgttt cctgccagca cgctgccgga aaaaagcagg caggcggata
    11821 ttattactgt cttatttttt ctcatcgtct gtttccccga aattatccag ccaggtcacc
    11881 ccgttttccc gtttcagaat aagaatgtaa tgtttgatat cagtgaaagg ttcggagtta
    11941 ccaatccatg ttttccgaat cccacggatt tccacacgac catattgtgg ccagacacga
    12001 acactggtct ggaaaaaagc agaattcaca ttatcttttt tgatgagctt tgcttcttcg
    12061 gccagaatca ctttcatctt attctgcgcg cccggacgga tatactgcag aagtgcctgg
    12121 tgagaagcat cgacgatttc cggtgagaca ttcaggcgca gggcaataaa cgccagcgcc
    12181 atctgctgca aaaaagaagc atcagcactg ttctgtgata ccgcaaaggg catattaaat
    12241 cccatcggtg tcacggcagt acgctgttca ttctgaaggc ggtaattatt caccccctga
    12301 atcacgttaa cagtcaggct gagaacaatg agcagggaca taaatataaa agcaatggcc
    12361 agtacacgac tggtacttaa gcgggcaccg tgttccatat aacaatccag gtatcagttc
    12421 tatttaatcc actgccggaa acacgaatca ggaacattat gaaaaatacc gcgcagcagg
    12481 actgttggca tataccagta aataagatcc cgtaaccaga agctgccccg cccttttttc
    12541 agttttttta tcccgaaata aaccagaacc gccgcaccaa taccgaacag atatttcgat
    12601 gttgtgatac cccagccaat acagatcgct gcggggatca gttcatccag tggcaggcca
    12661 aaccagcggc tctggttggt cagtgtttcc gggaaacgat atttcttaag tttattctcg
    12721 tctcccgaca tcgttttatt tcctgtcaga ggccaacgac ggtcatacct acagcaataa
    12781 atacagagat gatggcaaaa ccggccagga acttgacgtt tttggtcatc atgtacatga
    12841 ccgccccgac cagcacttca gccagaacga cccatttaac aatactggag tccttaccaa
    12901 aggtggcttt gacggtgtca ttcccggact gcatgaggtc ctgaccgcca gcagccaggg
    12961 ccatctgagg gaagaacatc atcagcacag ctgccgggat cacagcacga accaggcgaa
    13021 gcaggttcag tttagagaaa aaagatgatt tctttttaac aggcgtaaaa gcccccggaa
    13081 cactaacaga agtattcatc aattacctcg gattgtatgt aaaaagatta aaactaaagt
    13141 tctttaaaag ttgattctct ctcttctgtc acctccctga atatctcttc gttataaaaa
    13201 tctggataac gttgcaaatg atcctgcaac ctcaccaatg cttcatttgt tttcgttctt
    13261 ccactgcgct ctctggctcc aagtaataat ttattggttg cctcatcaag ggtcagtaaa
    13321 acctttctac ttggagcagc tcttaaagca cctttgatca ccacacctcc cgtacataaa
    13381 agagatttag tcaaaat
//
LOCUS       DAZFFI010000120        13364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFFI010000120 DAZFFI010000000
VERSION     DAZFFI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.120"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..799
                     /gene="yjiC"
                     /locus_tag="ACHJ12_004070"
     CDS             11..799
                     /gene="yjiC"
                     /locus_tag="ACHJ12_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4991780.1"
                     /translation="MNNHFLHHPKVDNELTRKYERARLDTENIYLLPLTRGNNHNYDG
                     KSVVEIRKLDISKESWPFNYVTETCRESDGITTTGRMLYRNLKITSALDEIYGGICKK
                     AHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGYISTLPVSQ
                     AKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGNDPIIGIEAYPGTAE
                     IHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(940..1713)
                     /gene="uxuR"
                     /locus_tag="ACHJ12_004071"
     CDS             complement(940..1713)
                     /gene="uxuR"
                     /locus_tag="ACHJ12_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4991781.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1928..3388)
                     /gene="uxuB"
                     /locus_tag="ACHJ12_004072"
     CDS             complement(1928..3388)
                     /gene="uxuB"
                     /locus_tag="ACHJ12_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4991782.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3469..4653)
                     /gene="uxuA"
                     /locus_tag="ACHJ12_004073"
     CDS             complement(3469..4653)
                     /gene="uxuA"
                     /locus_tag="ACHJ12_004073"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4991783.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4993..6336
                     /gene="gntP"
                     /locus_tag="ACHJ12_004074"
     CDS             4993..6336
                     /gene="gntP"
                     /locus_tag="ACHJ12_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO4991784.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6579..7481)
                     /gene="fimH"
                     /locus_tag="ACHJ12_004075"
     CDS             complement(6579..7481)
                     /gene="fimH"
                     /locus_tag="ACHJ12_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4991785.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7501..8004)
                     /gene="fimG"
                     /locus_tag="ACHJ12_004076"
     CDS             complement(7501..8004)
                     /gene="fimG"
                     /locus_tag="ACHJ12_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO4991786.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8017..8547)
                     /gene="fimF"
                     /locus_tag="ACHJ12_004077"
     CDS             complement(8017..8547)
                     /gene="fimF"
                     /locus_tag="ACHJ12_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4991787.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8557..11193)
                     /gene="fimD"
                     /locus_tag="ACHJ12_004078"
     CDS             complement(8557..11193)
                     /gene="fimD"
                     /locus_tag="ACHJ12_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4991788.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGVQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNGSGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11260..11985)
                     /gene="fimC"
                     /locus_tag="ACHJ12_004079"
     CDS             complement(11260..11985)
                     /gene="fimC"
                     /locus_tag="ACHJ12_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4991789.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12022..12561)
                     /gene="fimI"
                     /locus_tag="ACHJ12_004080"
     CDS             complement(12022..12561)
                     /gene="fimI"
                     /locus_tag="ACHJ12_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4991790.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSEHVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12626..13174)
                     /gene="fimA"
                     /locus_tag="ACHJ12_004081"
     CDS             complement(12626..13174)
                     /gene="fimA"
                     /locus_tag="ACHJ12_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4991791.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3254 a         3453 c         3226 g         3431 t
ORIGIN      
        1 ccaattctat ataaataatc acttccttca tcatccaaaa gtcgacaatg agttaacacg
       61 taaatatgaa cgtgcacgcc tggatacgga aaatatctat cttctgcctc ttactcgtgg
      121 caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaacttg atattagtaa
      181 agagtcctgg ccatttaatt atgtgacaga aacatgcaga gaatccgatg gtattacaac
      241 aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg aaatatatgg
      301 tggtatatgt aaaaaggcgc atgctgctac agaattagca gaaggtttac ggctaaattt
      361 atttatgaag agccctttcg atcctgttga ggactataca gttcatgaaa taacattagg
      421 ccctggttgc aatgttccag gttacgccgg aaccacgata ggatatattt caacccttcc
      481 agtttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct atattgatca
      541 gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg cattacttaa
      601 tgctaatatt ggaatgggaa atgacccaat aattggtata gaagcctatc ccggtaccgc
      661 ggagatccat gcaaaaatgg gatataaagt tatcccgggt gatgaaaatg caccactcaa
      721 acgtatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa atggtgaatg
      781 gaattatatt ggtaaataac taccaggaac aatgagtact aatattcagg ttatacagat
      841 atttaaacaa taccctgcaa tttacaaagt aatactcatt gcgacttctt aatttaagaa
      901 agcgcaagga acgtttaccc ttgcgcttat tataataagt caggcgtcga ctttatccag
      961 cggccatgaa tcaaacagat agccatcaaa ataaatatcg tcaacgttcg agaattccag
     1021 cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg ctcgggcatc
     1081 ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt tgcgatacag
     1141 gctgtcatcc agatggctgt gcaactggat ccacattgga ttgttttccc gccactgcca
     1201 ggactgacgg aacagctcca ccagcatgct gttatgcgtt gcttcggcaa tagcgagatg
     1261 gaactgcatg tcaccgcttt cgctgctgcc tggcgcactg gaagccagtt cacgctcttc
     1321 cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca aagcggcaaa
     1381 ctcggcgatg ttgctctcca ataactgccg cgcctgtaac agctcaaaag gaccggcatc
     1441 gttgcagacg ttggcatccg gactgtctgt gttctggctg cctgagttat caagaacata
     1501 gataccggca ccccggcgta cttccaccag ccctttgatc tccagcatga tcagcgcttc
     1561 acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg gcggcagccg
     1621 ttcgccaggg ttgtacggcg tctttatgat cagatcgcgg atcatcgccc cgacttcctg
     1681 gtaaggtctt tgcgcagagg tggcagattt catggtgttg tgtccgttag gtgcggttaa
     1741 tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg caattttacg
     1801 gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac aggtgggcaa
     1861 aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct gtagggtaat
     1921 cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg
     1981 ccgccgtcac tgcgccaaca aagtcggcat tctgcggcag atcatcggca aaaataccgc
     2041 tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgtta atcttctgga
     2101 actcggccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc acgccctggg
     2161 tgtaacgcat ccagccagcc acgcccagcg ccaggtgacg ccagctaccg ccgttttgca
     2221 ggtgcagacg caccggatcc agcagacgct gcggtaactt ctggctgccg tccattgcaa
     2281 tctgccaggt acggtgacgc agagatgggt tgctgaaacg ctcgatcagc agcgtcgcat
     2341 aggcgttcag gtccgtacct tccggcatcg acagcgttgg cgcttgttcc tgcatcatca
     2401 gggcaaaggc cgctttgcga taatccgggt tagtcatggt gtcggcaata gtttcatagc
     2461 cgccgaggta accgaggtac gccaggaaag agtggctgcc gttcagcata cgcagcttca
     2521 tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc cactttatcc cagtccgggc
     2581 gaccattaac gaagttatct tcaatcaccc actgacggaa cggttcgcag gcaatggcgc
     2641 acgggtcgta aacacccagc tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg
     2701 gaacgatgcg gtcaaccatg gtgcatggga aggtgacgtt ctcttcaatc catgccgcca
     2761 gctgcggatc gcgtgcctga gccagtccca gtaccgcgac cttcgccaca tgaccgtttt
     2821 cacgcacgtt atcgcaggac atcaccgtaa acgctttcaa ccctttttca cgacgcagac
     2881 gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt
     2941 gcttgatcag tgggttattg agatccagtt gaccgcttgc cgcatcagcg cagtagcctt
     3001 tttccgtgac cgttagagag acaatcgccg tttgcggacg cgccatcgcg ttgagaatac
     3061 cttcgcagcc atcgatttcc ggatgcagcg cttctttcat cgaaccgata attttcagct
     3121 cggtgctctc tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt ttcaggtttt
     3181 cgatcagcac gcggtcgttg cctggcatca ggttaacttc gcagatgccc cagtcgctgt
     3241 cggtgctttc cagcagatga tgggtataca gcgcctggtg cgcgcggtga aacgccccgc
     3301 aaccgagatg cacaatgcgt gattccagac gagaatgatc ccatgacggg cgggcaaccg
     3361 gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt caaagagtga
     3421 cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtggatatt aacggctaaa
     3481 gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt tcagacgacc
     3541 aattgcggag taacctgggt tggttttctt cttcaggtcg tccagcatct gatgaccgtg
     3601 gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt gttcttcttc
     3661 aacaatcgct ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg
     3721 gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg
     3781 accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt aggaaccggt
     3841 gcacatggta aaaccgtttg ccatgctgtt tacggtatca accatccact gcatatcttc
     3901 aatggtggaa acaatgcgcg gcaggccgag gatcgggcgc ggcggatcgt ccgggtgaac
     3961 agccatacgc acgccaactt cttcggcaac cggaataatc gctttcagga agacggcaaa
     4021 gttttcgcgc agtttggctt tgtcgatatc tttgtacagc tccaggtgct tacggaactg
     4081 gtcgagggtg tacccttctt ccgcgcccgg cagaccagcg atgatgttac gggtcagacg
     4141 cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct gcctgagcaa tttcttcttc
     4201 ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg cagcgaattc
     4261 gatctggtcg aagcgcagag ctttggagcc gtctggcagc acgtattcga ggtcagtacg
     4321 ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccac actgcgccag
     4381 gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag tgtgggtttt
     4441 gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg cgtcttcaac
     4501 gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg ggatatggtg
     4561 cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag aaaccggatc
     4621 gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac atagtgggtt
     4681 ccacagcaaa gagacgcgga accctggagt tgtttaggta tccggatgaa caaccagacc
     4741 gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg gtaagacatg
     4801 tcaacccgaa ttctgaaatt ggttaaccac atcacaagaa ttttactctc ctggttgttg
     4861 ctgcaaattg cagcaaaagg ttatcgatgg ttattgatca gaggatgtga cattcatcgc
     4921 aacaatggtt gaccaattta cataacatgt cgcccaaaat aacaacggtt caaccaggta
     4981 aggtaaaacg ttatgcatgt gcttaacatt ctctgggtag tattcggcat tggcctgatg
     5041 ctggtactga atttgaagtt caaaatcaat tcaatggtgg ctttgttggt ggcggcgctg
     5101 tccgtcggga tgctggcggg catggatttg atgtcgctgc tgcacactat gaaagcgggc
     5161 ttcggcaaca cgctggggga actggctatc atcgtggtgt tcggtgcggt catcggtaaa
     5221 ttgatggtcg actccggcgc ggctcaccag atagcgcata cgctgctggc gcgtctcggt
     5281 ctgcgctatg tacagctgtc ggtgattatc atcggcttga tttttggtct ggcgatgttc
     5341 tatgaagtgg cctttatcat gttagcgccg ctggttattg ttattgccgc cgaagctaaa
     5401 attccgttcc tgaaactggc gatcccggca gtagcagctg ccactaccgc acattcactg
     5461 ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga tatggggatg
     5521 gtttatatct atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg tctgatcctg
     5581 ccgaagttcc tcggcaatct tgagcgccca acgccatcat tcctgaaagc agatcaaccg
     5641 gtagatatga ataatctgcc ctctttcggc gtttcgattc tggtgccgct gatcccggcg
     5701 atcattatga tctctaccac catcgccaat atctggctgg taaaagatac ccctgcctgg
     5761 gaagtggtta actttatcgg ttcctcgccg attgcaatgt ttattgcgat ggtggttgca
     5821 ttcgtactct ttggcaccgc acgtggtcat gacatgcagt gggtgatgaa cgcttttgaa
     5881 agcgcggtga agagtattgc aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag
     5941 cagaccatca tcgacaccgg cattggcgac accatcggca tgttgatgtc ccacggcaat
     6001 atctcgccct acatcatggc atggctgatc actgtgctaa ttcgtctggc gacgggtcag
     6061 ggtgtcgttt cggcgatgac cgccgccggg attataagtg ctgcaatcct agatccagca
     6121 actggtcagc tggttggcgt gaatccggcg ctgctggtac tggcgacggc tgcgggttcc
     6181 aacaccctca cccacattaa cgatgcatct ttctggttgt tcaaaggtta ctttgacctg
     6241 tcagtaaaag acacgttgaa aacctggggt ctgctggagc tggtcaactc cgtggttggg
     6301 ctgattattg tgctgattat tagcatggta gcgtaaaagg acaccagagc ctgccaatgg
     6361 caggctctga ctgatgacaa agtctaaaaa tgtgcccaga cagtcctctc gcccctctgg
     6421 ggagagggtt agggtgaggg gaaaagacca gcaaagtgcg ctttgtttat gccagatgcg
     6481 acgctaacgc gtcttatctg gcctacaaag ggctaacgtg caggttttta gcttcaggta
     6541 atattgcgta cctgcattag caatgccctg tgatttcttt attgataaac aaaagtcacg
     6601 ccaataatcg attgcacatt ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc
     6661 gttaatccca gacttaccgc cgaagtccct actgctccta acgataccgt gttattcgct
     6721 ggaataatcg taccgttgcg cgtcaactgt acgccgacgc cctgcgctgg tgaaaacgac
     6781 gcggtattgg tgaaaatcga gttgcccgca tctgcggttg tgccggagag gtaatacccc
     6841 aggttttggc ttttcgcaca ataaacggta agaggaattg gcactgaacc agggtagtcc
     6901 ggcagagtaa cggtgacatc acgagcagaa acatcgcagc cgccagtagg caccaccaca
     6961 tcattattgg cgtaaatatt ccacacaaac tggaaatcat cgctgttata gttgttggtc
     7021 tgtcgcaaaa taagcacggc aattaatgag ccagctttaa tcgccacccc gcccgcactg
     7081 ctcacaggcg tcaaataaag cgccaccggc cacggcttat ccgttctcga attataaaca
     7141 acccgcggcg tttcgctggt ggtcggaaat ggatagctac tgccactata ttttacggtc
     7201 ccggaaaaat tagataacac gccgccataa gccgagcctc gttgcagtgt gacatagtct
     7261 gtaatggttt ccggataatc gttatggcaa aagatttgcg tcgaaagatc tacgaccagg
     7321 ttttgcccca cattcacggc aggcgcaagg tttacataaa cattagcgct gccaccgcca
     7381 atagggatag ctgtaccatt ggcggtttta caggcgaatg accaggcatt taccgaccag
     7441 cccatcagca gtacagcaaa cagggtaata actcgtttca ttacaatcat ctcttcgggt
     7501 tcagctgtag gtataggtga tgctaatcac tgcctgaatg gttccctgag tggctccgcc
     7561 atttactgtc aatgctctga cctgtaacgg gaagtgcgct gattgtgagg aatcatccac
     7621 ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca ctgtcatcct gtagctctaa
     7681 ctggatgttt tgcgcggtcc cctggttttt ataatatccg gtactgtcgg ctgccccgct
     7741 gaagctggca gtgaccctcg acgttcccac cggacaatta gtcaactcaa gcgcaacatc
     7801 atgccaggcc gatgccgccc cggcagacat cagactgaaa gaataaagat cgccgagatc
     7861 aaccgtggca ttggtggtgg aaaccgtgca cggtttggcg acgaccttac cgttcaccgt
     7921 gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc gccaatattg ccgccaataa
     7981 atacccacgt ttgcgccatt tcatgagcat ctccagttac tgatattcaa gagtgaaggt
     8041 agccgtggca ttgatatgcc ccgcagtgac aggcacctgt gtcgccatta gccgggcgta
     8101 aaaattcaac gtatttggtt tacccggcgt cagggtcgtc cacgaaatcg cggacgatgg
     8161 agcattaagg ggtatctgat tttgctgctc attcagaagc tgtattccca gtcccgaagc
     8221 cgctgatacc gtattttcaa gtgcaagcag gttggcattg tggctatctg caacgccggt
     8281 aaacccaacc tttacggcag aaacggcgtt accacagggt gacagcaaaa tacgaaatgg
     8341 aacgacagga gtcgttgcgc caatgttgtt aaattgcttc gccgcgtttt ccatcagatc
     8401 aacagtaaaa ttggttgatt cagcggccac actacagccg ttatctctga catagccgcg
     8461 gatagtaatc gtgctatccg cagccaaaac gcgacttacc gccagccaca aaaaagcgca
     8521 cagaagataa aaaggtttgt ttctcatcac gcccccttaa cgacattcag ctgatagctg
     8581 ggttaataac tgctgctgac tctctggtgg cagttgataa ttggcgatac agtgagcatt
     8641 ttcctcttct ccccatttca cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac
     8701 ctgaccatta tccgcaacaa tgccgctact ctggctactc tctgatgtca ccatcgcccc
     8761 aaacggcagc ggcttattat tgtgggttag cgtcatgagc agttttatcc caacgcgcgc
     8821 tttaaactct gctcgcacga tcgccccacg agtgggaaca acgttagcga ccgcgttatc
     8881 taaatcgacg ttatcagcca gggtattggt atccagcgcc actctatttt cccgatattc
     8941 agtggcataa ggcagcacgg cataaccacg ccagtcggta cgcacccccg tctggttttc
     9001 gacttttgca tcttttgcgc caggcgcttt aacaagcacc accgtatcgt ttaacggctg
     9061 ccccagcgtt acgccattgg catgagccag taccccaccg ctgactccgt aatagagctg
     9121 cttaatatca tcactatggc tgtaaccgat attggcattg ccgtaaccac cgcgataatt
     9181 cagcgtggcg tagcctgtgc ttccgctatt accatcgcct cccccggcat agccggtttg
     9241 cacgctatag ctgaggttgt tgtcttccag caacgtaccg tatacaccag ccagattggt
     9301 cattcgaccg ttgagatcgt gtgacatgct gtagctggca ctggcatgtc gccactgaga
     9361 tttactgtca gaacgcagcc agtggctgaa aggaatattg acgttaagcg ctaacatctg
     9421 atcacgtcct ttttgccagg cgtttttcgt caggctatag ctgagcgtcc agttgatatc
     9481 ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg acattactcg ttccccaata
     9541 agtttgatgg ctaccactca aatacagtgt tgatgagcgc ccgagttgct gagtaacggt
     9601 gagttgtaat ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga atttcggctt
     9661 aacctgaata actccgtcct gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt
     9721 atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa cccactaact gaatattcgt
     9781 gcctgaccca ttgagcgatt tgttatagag aaaacgcacc gattgtccgt catgctgact
     9841 gtcatcggga agtgtggaat tagcctgcgt catatccaca gacagagcgc ccagtgcccc
     9901 catgtttttc ccgataccga aattaaaagc acgataacga tccgccagtt gcgttccacc
     9961 atatattgtc cagccagccg gaaggccgtg gagtaatgta ctctggaaaa agcggggttt
    10021 ttcctgctgc gcatttccac tacggtattc tcctgccgta atggaataac gagtatgccc
    10081 ttcacgttgc aaaagcggga ctgacgaata gggtacggta aaaatctgcg tgctgccgtc
    10141 agcctctttg atcgttacct gcaagtcacc actattacct gcggcataga tatcgttgat
    10201 ggtaaaaggc cccggtggca ccgtactatt ataaatgtca tacccatttt gtttaatagt
    10261 gacctgtgca gtaccacgag caataccgtg gatcaccggg gcaaatcctc tttggctatc
    10321 gggtaacata ttgtcatctg aggccaattg tacgccgcga aagttaatac catcgaaaat
    10381 atcgccctga gtataaccat cacccagcgt cagccgggaa cgtaacggta ttatgtctcg
    10441 ctcaagccag gtattgatat gctgccattt atttttgcta cctgatgatc tgtcgctact
    10501 gttataactc caggtggtat tgtcgcgtaa acgccacgca ccaatattta acccactctg
    10561 taggtttaaa tatgcataat ggctgttacc cccaatccga ttctgtacac tatttccgct
    10621 gaaattataa ttgagcaatc cggcattaat accgggatcc cataactcag gaggaatata
    10681 accacgcgcg cgattactca taaatgcctg agggatcgtc aggttgagtc gctgctgacc
    10741 aacatctaaa tgcgcagtag cgtcatggat cattgaggtt aatggcacgc aggcatcatc
    10801 cgccagcaga ttcataccgg agacagaagc cgtattcagc cccatactgg caagctgcgc
    10861 gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc gtattaaatg tgacatcacg
    10921 cgttgccata taaccattat tcaaatagat atcgacacga tacgtccctg gcggtaattc
    10981 ttgcccattt tcaaaacgcg ataaatcagc cacagcctgg ggatcatccg ctaaaaaacg
    11041 cgggttaaaa tagagttcgg cagatgacaa aggtgcctgt gcggcaaaag cacaggcaac
    11101 aaagagccgg acaaaaaaac cagccaaacg atgcttacga atatgcaagc attgtgtgtt
    11161 tcgctggtaa agtcttaaat tcagatatga cattattcct ccggtttaaa atcacccggc
    11221 agtcaattct ttttattcag gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat
    11281 tttgggggta agtgcgccat aatcatttat tgttcggtaa gtaatattgc ttcctgcatc
    11341 agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt caagaacccg
    11401 ggttccggca ttcaactctg ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag
    11461 ggaattcgcg ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa
    11521 tttagccgga cgatagtaca gtttaatgcg gctgataatt gcgagctgta gcgtattctc
    11581 agtcaatttt gatttatcca ttgacggaat cgctttaaca ttcatccaga ataaactttc
    11641 ccgatcctgt ggcaattggt tattagttgc atcaagaata cgcaaggtat tctctctttt
    11701 tcccttcatc gcaaacagag gaggcgtcac gataaaacga ccatccttta caccatcggc
    11761 attttccacc catgattgaa ttaaataggt actattttca tcattatttg tcacggcaag
    11821 ttgcacttgt ttttgccctg ccggataaat tacgcgagtc gcacctaagg ccactcccgc
    11881 ttcagcgcgt ccggcaacca tcattgccat gaacatcagg atacctgcca gcaagcagaa
    11941 tgttatttcc tgcgatttcc ttacattgac gtttttatta ctcactgtcc tgttcctgtt
    12001 atcacattat ctgctgaaca attactgata ggttaaagag aaccaggcct gtgcattggc
    12061 gatgccgcca gtaacccgac gcccggtagc acgatatttg gcgatgaaat gtagcgaagt
    12121 agagcctgag taaagccgtt tccagtttgc tggaggacga ttaatcggta cgaggtttcc
    12181 ttcatcatca aacaacgcta cgccaatatt tgtggctatc cctggcccct ctccaacgga
    12241 aagcacatct ggatttttac catccgcgac accgtgaaac gccacaccca catgttcact
    12301 caccaccgtg ctacattccc gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc
    12361 aaccgcatga aaccggttac tgctgatttg ccccatattg accgtcattt gtttatcacc
    12421 ggcttcaatc cggcaagttt ccgcaataat gacgccctga aattgcatat ttccgccggg
    12481 caacgtggta ttccatttat ttccggccag agcaaacatt ggcagcaacg acgctaatag
    12541 aaataatctc ttccttttca tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt
    12601 aatgacgtcc ctgaacctgg gtaggttatt gatactgaac cttgaaggtc gcatccgcat
    12661 tagctgcacc cggggttgcc tcgccgattg cataataacg cgcctggaac ggaatggtgt
    12721 tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc accgtccagc gtcagagcat
    12781 tgcctgttct gtccaggatc tgcacgccaa cgtttgttgc actacctgca gcagaactct
    12841 gcagagccag aacatcagta cgcgtagcat caattgccgt acctaagaag gcaacagcgg
    12901 cttttgtggc aacagtggta tcgcaatcat tcagctgaat gttaaaacca acggcagagc
    12961 tggttgctcc agcctgcttc aggctagcgg tacgaacctg tcccaactga acggtttgat
    13021 caacagagcc ggcatcaacc gcacaagcgg cgttaacaac ttccccttta aagtgaacgg
    13081 tcccaccatt taccgtcgta gtatcggcca gagccgctgc ggaactgagg gacagagccg
    13141 acagaacaac gattgccaga gttttaattt tcatgctgct ttcctttaaa aaaactattt
    13201 ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa
    13261 cactgcacag ttttccccca aaagatgaaa catttggggc cattttgact catagaggaa
    13321 agcatcgcgc acaaactttt tcagtttatt tgctggctta atat
//
LOCUS       DAZFFI010000121        13219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFFI010000121 DAZFFI010000000
VERSION     DAZFFI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.121"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..295
                     /locus_tag="ACHJ12_004082"
     CDS             <1..295
                     /locus_tag="ACHJ12_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFO4991792.1"
                     /translation="GFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFG
                     IALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(436..762)
                     /gene="ypeC"
                     /locus_tag="ACHJ12_004083"
     CDS             complement(436..762)
                     /gene="ypeC"
                     /locus_tag="ACHJ12_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4991793.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWCDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(877..2133)
                     /locus_tag="ACHJ12_004084"
     CDS             complement(877..2133)
                     /locus_tag="ACHJ12_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4991794.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2337..3302
                     /gene="glk"
                     /locus_tag="ACHJ12_004085"
     CDS             2337..3302
                     /gene="glk"
                     /locus_tag="ACHJ12_004085"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4991795.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3521..3847
                     /locus_tag="ACHJ12_004086"
     CDS             3521..3847
                     /locus_tag="ACHJ12_004086"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4991796.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3869..5116
                     /locus_tag="ACHJ12_004087"
     CDS             3869..5116
                     /locus_tag="ACHJ12_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4991797.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5131..6216
                     /gene="ypdF"
                     /locus_tag="ACHJ12_004088"
     CDS             5131..6216
                     /gene="ypdF"
                     /locus_tag="ACHJ12_004088"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4991798.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6216..7253
                     /gene="ypdE"
                     /locus_tag="ACHJ12_004089"
     CDS             6216..7253
                     /gene="ypdE"
                     /locus_tag="ACHJ12_004089"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4991799.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7278..9773
                     /gene="ptsP"
                     /locus_tag="ACHJ12_004090"
     CDS             7278..9773
                     /gene="ptsP"
                     /locus_tag="ACHJ12_004090"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4991800.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9776..10633)
                     /locus_tag="ACHJ12_004091"
     CDS             complement(9776..10633)
                     /locus_tag="ACHJ12_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4991801.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10646..11380)
                     /gene="ypdB"
                     /locus_tag="ACHJ12_004092"
     CDS             complement(10646..11380)
                     /gene="ypdB"
                     /locus_tag="ACHJ12_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4991802.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11395..13092)
                     /gene="ypdA"
                     /locus_tag="ACHJ12_004093"
     CDS             complement(11395..13092)
                     /gene="ypdA"
                     /locus_tag="ACHJ12_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4991803.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3101 a         3389 c         3608 g         3121 t
ORIGIN      
        1 gggattcatt cgcttccata tcccgctgtg ggtgcgtcgt acagtcacca tgttgccgtc
       61 atttattgtc attctgatgg gattagatcc gacacggatt ctggttatga gtcaggtgct
      121 gttaagtttt ggtatcgccc tggcgctggt tccactgctg attttcacca gtgacagcaa
      181 gttgatgggc gatctggtga acagcaaacg cgtaaaacag acaggctggg tgattgtagt
      241 gctggtcgtg gcgctgaata tctggttgtt ggtggggacg gcgctgggat tgtagttgaa
      301 tgagcgtcgc atctggcact attggcggat gcggcgtaaa cgccttatcc gccctacgcg
      361 ttaaagagcc ggtttgtagg cctgataaga ctcgctagcg tcgcatcagg cattgtgttc
      421 cattggcgaa tgtgtttaat ggcggtgtcc atgtccgcgg ccttttccgc gatgatcgtc
      481 acgatcgcgc cagccttcac gatagccacg ctcatacgct ttacgcttat cccagccacg
      541 atgatagcca ttatcatgac gccaccagcg gtttttacgc cactcataat tgcgatgcca
      601 gtagtcacgg tcgcaccagt gaccaccgtc ccagtaatta ccgtaatgat cgcgatcgcc
      661 aatttgtaat ttgattgatg gcagtagggt gatttcacct gcgtttgctg caagcggggt
      721 aaatgccatc agggcggccg ccagaaacag tgacctgaac attgttattc tccttcacga
      781 tcgaagccgt cagcggcctg ttaacgcaat attacgggga ggtaaagccc cgtatcatcg
      841 ccataactct taaatcataa aggagagcat tttttgctaa acgttatcgt ggggtggctg
      901 ttgcttcgga cgattgacca tcatcatcgg cttacctgct aataacagcc atgccggaag
      961 catgacgata cagagcagcg gtagcaatgt ggtattgggt acaacgaccg ccgccataaa
     1021 aagacttaac cagccatcgc gtgttaccac cagcacgatg ccgagaatag cgcaagaaac
     1081 ggttattgcc gctggtacgg cgggtacgtg ctcatgcagc atcaacccta atgccacgcc
     1141 gacaaacact gccgggaaga ttcgcccacc gcgaaagcca ctggcggcag caacgaccag
     1201 ggcggcaagt ttaattaccg ccagcaaaaa gtaatcgctg gtgctgaaag cctgatttgc
     1261 caccatctgc tgcatctcat ccagcccttt aaacagcgaa actggtccac caataacccc
     1321 cagaataccg agaataaatc cgccaatacc cagcacgagc accggatttt tcatttgatt
     1381 catcatcgcg tgcaaccgtg gtaagcacca tacggcgacc atccctgctg cgatggcaat
     1441 cgccgcgaca attgcaccgc tgagaatatc ggtcatttcc atctgtccgt aatgcgcaat
     1501 gggcagtgaa aaatggggat ggaaaaataa tccggtagta agtgcaccag ctgctgccgc
     1561 cattaatggc gcaaaaagac gatcccatag cggaacttca ctactgccat ttaaggtttg
     1621 cgaaaatatc aacgccgccg caacaggtgt accaaacagc gcgccgatgg ttccggcaga
     1681 ggctaaaata gtccactcca ttcggttgac gcgcggtaac agacgagcgc caatggcaac
     1741 cgcaagggcg atattgacgg tcatgatcgg atgttccggc cccaggctga cgccgccagc
     1801 aagaccgaga attaatgcta cgataagtcc tggtagcgca gagggcggaa ctggtgcgcc
     1861 gatcagcggt tcacaggcgg ggtctggtcc ggcatgaccc tggctgaaac ggataaccaa
     1921 ccccaccgca atacccgtta gcgttaatac accgatgatc cagagaggtg aatcctgggc
     1981 tatccccaga gttcccggca gtcgttgcca gagcaaattc tgtaatgccg aggcgatttt
     2041 catcaccaca atcagaataa gactggacgc aatcccaatt gccacggcgg ggagcgataa
     2101 taacaacatg gttctggctc gcggatggag catgttctat tccttatgcg gggtcagata
     2161 cttagtttgc ccagcttgca aaaaggcatc gctgcaattg gtgctgaaac gataaagtaa
     2221 ttgtgtgacc cagatcgata tttacaggga gcctgccttt ccggcgttgt tgttatgccc
     2281 ccaggtattt acagtgtgag aaagaattat tttgacttta gcggagcagt tgaagaatga
     2341 caaagtatgc attagtcggt gatgtgggcg gcaccaacgc acgtcttgct ctgtgtgata
     2401 ttgccagtgg tgaaatctcg caggctaaga cctattcagg gcttgattac cccagcctcg
     2461 aagcggtcat tcgcgtttat cttgaagaac ataaggtcga ggtgaaagac ggctgtattg
     2521 ccatcgcttg cccaattacc ggtgactggg tggcaatgac caaccatacc tgggcgttct
     2581 caattgccga aatgaaaaag aatctcggtt ttagccatct ggaaattatt aacgatttta
     2641 ccgctgtatc aatggcgatc ccgatgctga aaaaagagca tctgattcag tttggtggcg
     2701 cagaaccggt agaaggtaaa cctattgcgg tttacggtgc cggaacaggg cttggggttg
     2761 cgcatctggt ccatgtcgat aagcgttggg taagcttgcc gggcgaaggc ggtcacgttg
     2821 attttgcgcc gaatagtgaa gaagaggcca ttatcctcga aatattgcgt gcggaaattg
     2881 gccatgtttc ggcggaacgc gtgctttctg gccctgggct ggtgaatttg tatcgcgcaa
     2941 ttgtgaaagc tgacaaccgc ctgccagaaa atctcaagcc aaaagatatt accgaacgtg
     3001 cgctggctga tagctgcacc gattgccgcc gcgcgttgtc gctgttttgc gtcattatgg
     3061 gccgttttgg cggcaatctg gcgctcaatc tcgggacatt tggcggcgtg tttattgcgg
     3121 gcggtatcgt gccgcgcttc cttgagttct tcaaagcctc tggtttccgt gccgcatttg
     3181 aagataaagg gcgctttaaa gaatatgtcc atgatattcc ggtgtatctc atcgtccatg
     3241 acaatccggg tcttctcggt tccggtgcac atttacgcca gaccttaggt cacattctgt
     3301 aaatccttcc tgttatatcg ggaggtaact ctcccgataa tcttttaaat catacagtta
     3361 attcaatttt tctttgtgtc ccctcacaag gccgacctgc gtcacacttc catacagcgg
     3421 gattaattct ccagtaaatg cattatttgt ctggtaacgg cgatttgttt tgcacgttca
     3481 taatttcact cgtcaactat cactgaacga ggaattaacg atgagtaaga aactgattgc
     3541 cttatgtgcc tgcccgatgg gcctggctca cacttttatg gccgctcagg cgctggaaga
     3601 agcggcggta gaagccggtt atgaagtgaa aattgaaacc cagggcgcgg acggtatcca
     3661 gaatcgcctg acggcgcagg atatcgccga agcaaccatc atcatccact ccgtggcagt
     3721 taccccggaa gataatgagc gtttcgaatc acgcgacgtt tatgaaatca ctttgcagga
     3781 cgcaattaaa aacgctgcgg gcatcatcaa agaaatcgaa gagatgattg cctctgaaca
     3841 acagtaatat tcgacaggga taattgttat ggccattaaa aaacgcagtg caaccgttgt
     3901 gcctggcgca tccggtgcgg cagcggcagt taagaatccg caggcctcta aaagcagctt
     3961 ctggggtgaa ctcccgcagc atgtgatgtc agggatttca cgcatggtgc caaccttaat
     4021 tatgggcggt gtgatcctcg ctttcagcca gctgattgct tatagctggc ttaaaattcc
     4081 tgctgagatc ggcatcatgg atgctcttaa tagcgggaaa ttctccggtt tcgacctctc
     4141 cttacttaaa tttgcctggc tgtcgcagtc cttcggcggc gtgttgtttg gttttgccat
     4201 tccgatgttt gctgcttttg tggcgaactc tatcggtggc aaactggcgt tcccggctgg
     4261 ttttatcggc gggttgatgt ctacccagcc gacgcaactg ctgaacttcg atcccagcac
     4321 tatgcaatgg gcgacctctt cgccggtgcc gtcaaccttc attggtgcgc tgattatttc
     4381 tatcgtcgcg ggttaccttg tgaagtggat gaaccagaaa atccagttac cggatttcct
     4441 gttagcgttc aaaaccacat ttttgctacc gattctttcc gccatttttg tcatgctggc
     4501 gatgtactac gtcatcaccc cctttggtgg ctggatcaac ggcggtatcc gtactgtgct
     4561 gactgccgca ggtgagaagg gcgcgctgat gtacgcaatg gggatcgccg cggcgacagc
     4621 aatcgacctt ggtggcccga tcaacaaagc cgcaggtttc gttgccttca gctttaccac
     4681 tgaccacgta ttaccggtca ccgctcgttc tatcgctatc gttattcctc cgattggtct
     4741 gggtctggcg accattattg accgtcgttt aaccggcaaa cgcctgttca acgctcaact
     4801 ttatccgcag ggtaagaccg cgatgttcct tgcctttatg gggattagtg agggtgcgat
     4861 tccgtttgcg ctggaaagcc ccatcaccgc cattccgtcg tatatggtcg gcgcgattgt
     4921 cggctcaacc gccgctgtct ggctgggcgc agtgcaatgg ttcccggaat ccgctatctg
     4981 ggcatggccg ctggtcacta acctgggcgt ctatatggcg gggatcgcgc tgggggcagt
     5041 cataactgcg ctgatggtgg tgttcctgcg tctgatgatg ttccgtaaag gcaaattgtt
     5101 aatcgatagc ctgtaagaag gaaaatccag atgacattac tcgcttcgct gcgcgactgg
     5161 cttaaggcgc aacaactgga tgcagtgctt ctctcctcac ggcagaacaa acagccgcat
     5221 ctggggatct ccaccggatc aggctatgtg gttattagcc gtgaaagtgc gcacattctg
     5281 gtagattcgc gctatttcgt tgaggtggaa gcccgtgcgc aaggctacca gctgcatttg
     5341 cttgacgcga cgaacacgct taccactatc gtcaatcaaa tcattgccga tgagcagttg
     5401 caaacgctcg gttttgaagg ccagcaggtg agttgggaaa ccgcgcaccg ctggaagtct
     5461 gaactcaatg cgaaactggt tagcgccacg ccggatgtgc tgcggcaaat caaaacgcca
     5521 gaggaggtgg agataatccg cctcgcctgt gggattgccg atcgcggagc ggagcatatt
     5581 cgccgcttta ttcaggcggg gatgagcgag cgcgagatag ccgctgaact ggagtggttt
     5641 atgcgccagc agggcgcaga aaaagcctct tttgacacca ttgtcgccag cggctggcgc
     5701 ggggcgctgc cgcacggcaa agccagcgac aagattgttg cagcgggcga gtttgtcact
     5761 ctcgattttg gtgcgctgta tcagggctac tgctctgata tgacgcgcac cttgctggtg
     5821 aatggcgaag gggtgagcgc cgaatctcac ccgctgtttg acgtctatca gattgtcctg
     5881 caggcacagc tcgcggcaat ctctgcaatt cgccccggcg tgcgctgcca gcagattgac
     5941 gacgccgcgc gccgggtcat taccgaggct ggatttggcg actatttcgg tcataacact
     6001 ggtcacgcta tcggcattga agtccatgaa gatccgcgtt tttcaccgcg ggacaccacg
     6061 acgctacagc caggcatgtt actgaccgtg gagccgggga tttatttgcc agggcaaggg
     6121 ggcgtgcgca tcgaagatgt tgtgctggtt accccgcaag gcgcagaagt gctctacgcc
     6181 atgccgaaaa cagtgttgct cacgggagag gcataatgga tttatcgcta ttaaaagcgt
     6241 tgagcgaggc agatgcgatc gcctcctcgg aacaggaagt gcggcagatc ctgctggaag
     6301 aagcggatcg cctgcaaaaa gaagtgcgat ttgatggtct gggatcggtg ctgatccgcc
     6361 tcaatgaatc gacaggtccg aaagtgatga tctgcgccca tatggacgaa gtgggattta
     6421 tggtgcgtag catctcccgc gaaggggcaa ttgatgtgct gccggttggc aacgtgcgca
     6481 tggctgcccg ccagctgcag ccggtgcgca tcaccacccg tgaagagtgc aaaattccag
     6541 gcctgcttga cggcgaccgg caggggaatg acgtcagcgc catgcgcgtg gacattggtg
     6601 cgcgctcgta tgacgaagtg atgcaggcgg gaattcgtcc aggcgatcgc gtcacctttg
     6661 atacaacttt tcaggttctc cctcaccagc gggtgatggg gaaagccttt gatgatcgcc
     6721 tcggttgcta tctgctggtg acgttactac gtgagctgca cgatgccgaa ctgcctgcgg
     6781 aggtgtggct ggtggcaagt tccagcgaag aggtgggatt acgcggcggg caaactgcca
     6841 cccgcgcggt gtcgccggat gtcgccattg tgcttgatac cgcctgctgg gcgaaaaact
     6901 ttgattatag cgcggctaac catcgccaga ttggtaacgg gccgatgctg gtgttaagcg
     6961 acaagtcgct gattgcgccg ccaaaactta ccgcctggat cgaaaccgtg gcggcagaaa
     7021 ttggcgtgcc gttgcaggcg gatatgttca gcaacggcgg cacggacggc ggagcggtgc
     7081 atttaaccgg caccggcgtg cccacggtgg tgatggggcc agcgacgcgc cacggacatt
     7141 gtgcggcatc gattgccgat tgccgcgaca ttttgcagat gcagcaactt ttatctgccc
     7201 ttattcaacg tcttacccgt gagacggttg ttcaactgac ggatttcaga tgatctcctg
     7261 attaacccgg agcggttatg ttaacgattc aatttctctg tcctttgcct aacggtctgc
     7321 atgctcgtcc ggcgtgggaa cttaaagaac agtgcagcca gtggcaaagt gaagtcacct
     7381 ttattaacca tcgccagaac gcaaaggccg atgcgaaaag ttcgctggcg ctgattggca
     7441 ccggcaccct atttaatgac agttgcagcc tgaacattag cggcagcgat gaagagcagg
     7501 cgcggcgcgt gctggaagag tacatccagg tgcgctttat cgacagcgac agcgtccagc
     7561 ctacgcaggc agaactgacg gcgcatccgc tgccgcgttc attaagccgc cttaacccgg
     7621 atttactgta cggcaatgtg ctggcaagcg gcgtcggcgt gggtacgctg accctgttac
     7681 aaagcgacag cctcgacagt tatcgggcaa tccccgccag tgtgcaagat tccacccggc
     7741 tggagcacag cctggcaacg cttgccgagc aactgaacca gcaattgcgt gagcgtgacg
     7801 gcgaaagcaa aactatcctc agcgcccatt tgtcgctgat tcaggatgat gaatttgcag
     7861 gcaatatccg tcgcctgatg acagaacagc atcagggact gggggcggcg atcatcagca
     7921 atatggagca ggtttgcgcc aagctttctg cttctgccag cgattatctg cgcgaacgtg
     7981 ttagcgacat tcgcgatatt agcgaacagt tgctgcatat cacctggccg gaactgaagc
     8041 cgcgcaacaa tctggtgctg gaaaaaccga ccattctggt ggctgaagat ttaaccccaa
     8101 gccagttttt gagccttgat ttgaaaaatc ttgcgggcat gattctggag aaaaccgggc
     8161 gcacctcgca taccctgatt ctggcccgcg cctcggcgat cccggtactg agcggcctgc
     8221 cgctggatgc gattgcccgt tatgccgggc aaccggcggt gcttgacgcc cagtgcggcg
     8281 tgctggcgat taacccgaat gacgcggtga gcggttatta tcaggttgcg cagacgctgg
     8341 cggataaacg ccaaaaacaa caggcgcagg ctgccgcgca gctggcctat tcccgtgata
     8401 acaagcgtat tgatatcgcg gcgaatattg gcaccgctct ggaagcacca ggcgcgtttg
     8461 ccaacggcgc ggaaggcgtc gggctgttcc gtaccgaaat gctgtatatg gatcgtgaca
     8521 gcgcgccgga cgagcaggag caatttgaag cctaccagca ggtgctactg gcagcgggcg
     8581 acaagccgat tatcttccgc acaatggaca tcggaggcga taaaagcatt ccttatctga
     8641 atattcccca ggaagagaac ccgttcctcg gctatcgcgc ggtacgtatt tacccggaat
     8701 ttgctggcct gttccgcact caactacggg cgattttgcg cgctgccagt tttggcaacg
     8761 cccagttgat gatcccgatg gttcacagcc tcgatcagat cttatgggtg aagggcgaga
     8821 tccaaaaagc gatcgttgag cttaagcgcg atggcctgcg tcatgcagag acgattactc
     8881 tgggtatcat ggtggaagtg ccgtcggtgt gttacatcat cgaccacttc tgcgatgagg
     8941 tcgatttctt cagtatcggt tctaacgata tgacccagta tctgtatgcg gtcgatcgta
     9001 ataacccgcg cgtatcgccg ctgtataacc cgattacgcc atcgttcctg cgcatgttgc
     9061 agcagattgt taccactgcg catcagcggg gcaaatgggt aggcatttgc ggtgaactgg
     9121 gcggtgaaag ccgttatctg ccgctactgc ttgggctggg cctggatgag ctgagtatga
     9181 gtagcccgcg tattccggcg gtgaaaagcc agcttcgtca actggatagc gaggcgtgtc
     9241 gggaactggc gcgtcaggca tgtgaatgcc gcagtgcgca ggaaattgaa gcgttactca
     9301 ccgcctttac gccggaagaa gacgttcgcc cactgctggc gctggagaat atctttgttg
     9361 atcaggattt tagcaataaa gagcaggcga tccagttcct gtgcggcaac ctcggcgtta
     9421 acgggcgcac tgaacatccg tttgagctgg aagaagatgt ctggcagcgg gaagagattg
     9481 ttaccaccgg cgttggtttt ggcgtagcga tcccgcacac caaatctcag tggatccgtc
     9541 attccagtat cagcattgcc cggctggcga aaccgattga ctggcagtca gaaatgggcg
     9601 aagtcgaact ggtgatcatg ctgacgctgg gtgctaacga agggatgaat catgtgaaag
     9661 tcttctcgca gctggcgcgt aaactggtga ataaaaactt ccgccagtcg ttgtttgccg
     9721 cgcaagatgc acaaagtatc ctgacgctgc tggaaacaga attaaccttc tgacgttagc
     9781 cctgaaaacg ggcgctgtac tctcccggcg tcagaccaaa ctgacgccgg aaaacgcgac
     9841 aaaaatagtc gctatccgga aaaccgcaac gctgtgccac ttcatgaatt gacagatgat
     9901 atttctgcaa aatcatcctc gccttcgcca ttcgcaccca acgcacatac tcgataaaac
     9961 gcatcgttcc atgctgagca aacagttttg acagatgatt gggcgtgata ttaaaaaact
    10021 gcgccacgct ctcgcgggtg agcggctggg cataattatc ctgaacccag ttacaaatgc
    10081 tgtgatagag aaaatcgccg cgcggttgcg aattacccgg cggcatattc accacgttgc
    10141 ggcaaagatg cagcaaactt aacaccagtg gctgaatgat attctgctcc agcggcgaac
    10201 ggctaagatg ggtgagggcg gtaagcatcg cttcaccttc accgcgttgc aggctgggaa
    10261 gctggatctg gcgagcagga tgcaacaacg acgtggtgcg gctatcgtaa aacgataacc
    10321 caagccaggt cggtgcgaac accaggctta acagcatcac cggtttgttg ttgatcggta
    10381 aattagccgc gcgtgccgga ataaacagca tttctccttc caccattcgc tgctgctttg
    10441 cttccagcag gttgccgtat tcgccgcgca gcacgatatc cagacgggga aaatcaatac
    10501 ataaactgcc caccggtaac gaggcaggct ggctggcaaa ccagacgcgc cccagtagtt
    10561 gcgggttaag caccaggccg ctgaacagat cggcgaaaaa ttgttgatcg gcgggcaagc
    10621 ctggtgcttt catgaaagtt cccgattaaa gatgcattaa ctggcgaaat tctttcactt
    10681 tgctgcgact gaccggcact tcaaaatcca gatctttcag tcgcagaatg taggtgttat
    10741 taaaccacgg ttcgatttcg cgtattttgt tcagattgac acaaaatgag cgatggcagc
    10801 ggaaaaaatg cgacggcggc agtttgctgc aaaattcggt aatgttcatc ggcattacgt
    10861 aggattcacg ccgcgtatag acaaaggtca ttttctcgtg cgcttcggcg taatagatat
    10921 cgttaattgg cgtgacgatt attcgctcat ctttcaccag attaatggtg tcattctcac
    10981 gcgttaccgt cgcggcaggc gtactgctgg tctgctgttg ttgccaggcc gcttccagtt
    11041 tttgcagcat cccggtaata cgtgactcct gatacggttt gagaatgtag tcaaacgcct
    11101 ccagttcaaa cgcttctacc gcatgttctt tccacgcggt gatgaacaca ataaacggtt
    11161 tatgggcgaa ctggctgatg ttttgcgcca gcaacacgcc atccagcgac ggaatattga
    11221 tatccagaaa aatggcgtcg acgcggttat gctgcaaaaa cttcaacacg tccagaccgt
    11281 cgtcaaaggt gccgacaatc tccatctggc tgtgctcttt aattagccag ctcagttcct
    11341 gttgtgccag gaattcgtct tcaacaatga tgactttcac aatatcactc cggctcaaag
    11401 caataacgta gcctgtgagg cgactggggt gcgttggtta ggaatgtaaa acgcaatttc
    11461 cgtccccggc tccaggcggc ggatatgcag cccctcgcca tacaataact tcacgcgatg
    11521 atggacattc agcaggccaa ttttattgcc cggcatttca ttcgcttcga cccgctcaat
    11581 cacctttgga tcgatgccgt gcccggtatc tcgcaccgca atgcgtaccc gatttccgca
    11641 ctctgcaacg ctgatggtga caacgccttt acctttgcaa ggctgaatac cgtggacaat
    11701 ggcgttctcc accaacggct ggatcagcag gctgggaatg cagcaattca cctcttcatc
    11761 aatatcatag ataaccgtca gcttgtcacc aaagcgggcc tgctcaatgg caatatagtc
    11821 tttaatttga tacagctctt ttttgatatc gatttgctca tcgtctttta attcaatgtt
    11881 atagcgcaga taacgcgaca gattaaagat cagctggcga gcagtatccg gattcagacg
    11941 gattgacgat gaaatagcgt tcagagcgtt aaacagaaaa tggggattaa ttttgctttg
    12001 cagggcgcga agctctgcct tatttgccat ttcacgtagc tgctcggcgc gtgaaacctc
    12061 cagttgcgtg gagatgattt gcgacagacc gaccgccatt tcctgtaatg acgaggtgat
    12121 ctgatgcgcg tggcagtagt aaattttcag cgttccggtc acgacccctt tctcccacaa
    12181 tgggatcacc agcatggaat gaatctctgg tgtgcggtgg gcttcatcat tgtttttaat
    12241 gatgattttt ccgtaattca tcgcctgacg ggtagtcggg ctaatgaagt catcgccatt
    12301 ctgatagtta tgttcaccca cgccaacata ggccagcaca tgatcggtat tagtaatcgc
    12361 caccgcatcg gcgtgaatgt catcgcggat aatttcgcag accttgcgta atgactcgct
    12421 attgacatgg cgaaacagcg gtagcgtttt gttggcgata tccagcgcca gcttggcctg
    12481 ccgcgcggcg ctggcctctt tttcgccctc aacgctttgc accagaagca caataaagcc
    12541 gatacagacg ctaccgagga tcattggaat gccgatttta gagacgatat cgatccccaa
    12601 cgcggtagtt ggtgcccaga caattaccag aatcatggtc aacgtctcac acaacatgcc
    12661 gccgagaata ccgacgcgcc agcgctgtgc tttggggatt ttcaggttta tccagcctga
    12721 tatacaaccc gccagaatgc tggtgataaa gcaggggatc gccgtcacgc cgccgatatc
    12781 aattaaatac cggtgaatac ccgcaatcac gccggtaatg atgcctaccc acgggccaaa
    12841 cagaatcccg ccggacatca ccgcgataat acgtacgttc accagcgagc cttctacggg
    12901 aacgccggac caggtgctga acagggcgaa cagcgagaaa atggcggtaa cggcgagcaa
    12961 ttcttttggg gagtgcgccg acttgtgcaa cagttcgcga aacaggcgga tacggatgag
    13021 aaagaacagg cagataagca ttaacgctgc ccgatcgaag accgccagca gcatgttgaa
    13081 tatttcgtgc acgggtgact ctgaataagg attaaacctc tatgataggt aacctgaagg
    13141 ctgatgacca gcaggccgtt tttgaggaaa aaccataaat ttaaaagggg attgaaagat
    13201 aaatggtgta ttagcaatg
//
LOCUS       DAZFFI010000122        13210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFFI010000122 DAZFFI010000000
VERSION     DAZFFI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.122"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..825
                     /gene="aphA"
                     /locus_tag="ACHJ12_004094"
     CDS             112..825
                     /gene="aphA"
                     /locus_tag="ACHJ12_004094"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4991804.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            936..1352
                     /gene="yjbQ"
                     /locus_tag="ACHJ12_004095"
     CDS             936..1352
                     /gene="yjbQ"
                     /locus_tag="ACHJ12_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4991805.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1356..1712
                     /gene="yjbR"
                     /locus_tag="ACHJ12_004096"
     CDS             1356..1712
                     /gene="yjbR"
                     /locus_tag="ACHJ12_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4991806.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1747..4569)
                     /gene="uvrA"
                     /locus_tag="ACHJ12_004097"
     CDS             complement(1747..4569)
                     /gene="uvrA"
                     /locus_tag="ACHJ12_004097"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4991807.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4823..5359
                     /gene="ssb1"
                     /locus_tag="ACHJ12_004098"
     CDS             4823..5359
                     /gene="ssb1"
                     /locus_tag="ACHJ12_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4991808.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5458..5739)
                     /gene="yjcB"
                     /locus_tag="ACHJ12_004099"
     CDS             complement(5458..5739)
                     /gene="yjcB"
                     /locus_tag="ACHJ12_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4991809.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6169..7755
                     /gene="pdeC"
                     /locus_tag="ACHJ12_004100"
     CDS             6169..7755
                     /gene="pdeC"
                     /locus_tag="ACHJ12_004100"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4991810.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7758..8081)
                     /gene="soxS"
                     /locus_tag="ACHJ12_004101"
     CDS             complement(7758..8081)
                     /gene="soxS"
                     /locus_tag="ACHJ12_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4991811.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8167..8631
                     /gene="soxR"
                     /locus_tag="ACHJ12_004102"
     CDS             8167..8631
                     /gene="soxR"
                     /locus_tag="ACHJ12_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4991812.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9177..10526
                     /gene="ghxP"
                     /locus_tag="ACHJ12_004103"
                     /gene_synonym="yjcD"
     CDS             9177..10526
                     /gene="ghxP"
                     /locus_tag="ACHJ12_004103"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4991813.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10678..12327
                     /gene="yjcE"
                     /locus_tag="ACHJ12_004104"
     CDS             10678..12327
                     /gene="yjcE"
                     /locus_tag="ACHJ12_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4991814.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12481..>13210)
                     /locus_tag="ACHJ12_004105"
     CDS             complement(12481..>13210)
                     /locus_tag="ACHJ12_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4991815.1"
                     /translation="FYYAIMEKILFDDCILDDSNFAQIKMADGTLNACSAMHVQFYNA
                     AMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLS
                     RVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSI
                     LTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTRQKFFEEDCTSHTG
                     MSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         3098 a         3224 c         3463 g         3425 t
ORIGIN      
        1 aaaaccgcag ataatccttc ctttccccgg cagctggcgt tatggtcaga tggtttttgc
       61 aacaaatctc acaataaaaa gtttcaacat actgactatt tagggaaaaa tatgcgcaag
      121 atcacacagg caatcagtgc cgtttgctta ttgttcgctc taaacagttc cgctgttgcc
      181 ctggcctcat ctccttcacc gcttaaccct gggactaacg ttgccaggct tgctgaacag
      241 gcaccaattc attgggtttc ggtcgcacaa attgaaaata gcctcgcagg gcgtccgcca
      301 atggcggtgg ggtttgatat cgatgacacg gtactttttt ccagcccggg cttctggcgc
      361 ggcaaaaaaa ccttctcacc agaaagcgaa gattatctga aaaatcctgt gttctgggaa
      421 aaaatgaaca atggctggga tgaattcagc attccaaaag aggtcgctcg ccagctgatt
      481 gatatgcatg tacgccgcgg tgacgcgatc ttctttgtga ctggtcgtag cccgacgaaa
      541 acagaaacgg tttcaaaaac gctggcggat aattttcata ttcctgtcac caacatgaat
      601 ccggtgatct ttgcgggcga taaaccaggg caaaatacaa aatcgcaatg gctgcaggat
      661 aaaaatatcc gaatttttta tggcgattcc gataacgata ttaccgccgc acgcgatgtc
      721 ggcgctcgtg gtatccgcat tctgcgcgcc tccaactcta cctacaaacc cttgccacaa
      781 gcgggtgcgt ttggtgaaga ggtgatcgtc aattcagaat actgacagag cgggagagcg
      841 tgacgctctc ccgaatgctg tttttttaat cacaccttta tcctttcgct gtcttgctgc
      901 aaactgatta agagagtttt atcaaggagc agcacatgtg gtatcaaaag acgctcacgc
      961 ttagcgccaa atctcgtggg tttcatctgg taacggatga aattctgaat cagctggctg
     1021 atatgccgcg cgttaacatc ggcttactgc atctgttgct gcaacatacc tccgcctctc
     1081 tgacacttaa tgagaactgc gatcccaccg tacgccacga catggagcgt tttttcctcc
     1141 gcaccgttcc cgacaacgga aattatgagc atgactatga gggagcagac gatatgcctt
     1201 ctcatatcaa atcctcaatg ctgggaacat cgcttgtatt gccggtacat aaagggcgta
     1261 ttcagaccgg cacctggcaa ggcatctggc tgggggaaca tcgcatccac ggcggatcgc
     1321 gtcgcatcat cgcgacacta caaggggagt aaaaaatgac catttcggag ttgctacaat
     1381 attgcatggc aaaaccaggc gcagaacaga gcgtgcataa cgactggaaa gcgacgcaga
     1441 tcaaagtgga agatgtactg tttgcgatgg tgaaagaagt agaaaatcgc ccagctgttt
     1501 cgctgaaaac cagcccggag ctggcggagc tgctacgtca gcagcacagc gatgtgcgtc
     1561 ctagccgcca tctgaataaa gcgcactgga gcaccgtgta tctcgacggt tcgctgccgg
     1621 attcgcaaat ctattatctg gtggatgcgt cttatcagca ggcagtgaat ttactaccgg
     1681 aagaaaagcg taaattgctg gtacaactct gaaaggaaaa ggccgctcag aaagcggcct
     1741 taacgattac agcatcggct tgaggaagcg tgccgtatgc gaagcttcgc actccgcgac
     1801 ggtttctggc gtaccggaga cgaggatttc gccgccgcca ctgccgcctt ctggtcccag
     1861 gtcgacaatc cagtcagcgg ttttgatcac gtcgagattg tgctcaatca ccacaatggt
     1921 gttgccctga tcgcgcagtt tatgcagtac gtcgagcagt tgctgaatat cggcgaagtg
     1981 cagaccggtg gtcggctcgt cgagaatata cagcgtctgc ccggtgccgc gttttgacag
     2041 ctcacgcgcc agcttcacgc gctgggcttc accaccagaa agtgtggttg cggactgccc
     2101 caggcgaatg tacgtcaggc caacgtccat caacgtttgc agcttacgcg ccagtgcagg
     2161 tacggcatca aagaactcac gcgcctcttc gatggtcata tccagcactt cgtggatggt
     2221 tttgcctttg tacttaattt ccagcgtttc acggttatag cgtttacctt tacactgatc
     2281 gcacggcacg tagatatccg gcaggaagtg catctctact ttgatcacac cgtcgccctg
     2341 acaggcttcg cagcgcccgc cacggacgtt aaagctgaaa cgtcctggcg tataaccacg
     2401 cgcacgggat tccggtacgc ccgcaaacag ttcgcgcaca ggcgtaaaca cgccggtata
     2461 ggtcgccggg ttagaacgtg gagtacgacc aattgggctt tggtcgatat cgatcacttt
     2521 atcgaaatgc tccagcccct gaatatcgcg atacggtgct ggttcggcga tggtcgcccc
     2581 attcaactgg cgttgggcaa tcgggaacag tgtgtcgtta atcagcgtcg atttaccgga
     2641 acctgaaacc ccggtgatgc aggtaaacag acccaccggc agcgtcagcg tcacgtcctt
     2701 caggttgttg ccgcgtgcgc ctgtcagctt cagcactttt tccggattcg ccggaacgcg
     2761 tttcttcggc acttcaatct tgcgtttgcc gctcatgtac tgcccggtca acgactccgg
     2821 caccgccata atcgcttcca gcggaccttc tgcgaccact tcaccgccgt gaacacctgc
     2881 gcccgggcca atgtcgatca catggtcagc ggcgcgaatt gcgtcttcgt cgtgctccac
     2941 cacaatcacg gtattaccga gatcgcgcag atggataagc gtacccaaca ggcgctcgtt
     3001 atcgcgctgg tgcaggccga tagatggctc gtccagcacg tacataacgc caaccaggcc
     3061 cgcaccaatc tggctcgcca gacggatacg ctgggcttca ccgccggaaa gcgtttctgc
     3121 cgagcgggaa agcgtcaggt aattcaggcc gacgttaacg aggaatttca aacgatcgcc
     3181 gatctcttta aggatttttt ccgcaatctt cgcccgttga ccagcgagtt tgagattgtt
     3241 gaagaattcc atcgcatgac cgatgctcat gtcggagata gcaggcagcg gcgtattctc
     3301 gacataaacg tggcgcgctt cccgacgcag acgcgtccct tcgcagctgg cgcatgggcg
     3361 attgctgata aacttggcta attcttcacg taccgcactg gattctgtct ctttataacg
     3421 gcgctccata ttgtgcagca cgccttcgaa cggatgacga cggatggagg tatcgccacg
     3481 atcgttcatg tatttgaatt caatgttttc tttgccagaa ccgtacaaca ccactttatg
     3541 cacgttcgcg ctcaggctgc cccacggcgc ttcgacgtcg aacttatagt gatctgccag
     3601 cgatttcagc atctggaaat aatagaagtt gcggcgatcc cagccacgga tcgcaccgcc
     3661 agccagtgac agctcggggt tttggatcac gcggtcagga tcgaaatatt gctgtacgcc
     3721 aaggccgtca caggtcgggc aggcacctgc cgggttgtta aacgaaaaca gtcgcggctc
     3781 cagttcacgc atactgtagc cgcaaattgg gcaggcgaag ttggcggaga acagcagctc
     3841 ttccgctttc gggtcgtcca tatccgccac taccgctgta ccaccggaaa gctccagcgc
     3901 ggtttcaaac gactccgcaa gacgttgggt aagatcgtca cgcaccttga agcgatcaac
     3961 caccacttca atagtgtgtt tcttttgcag ttccagtttc ggcggatcgg aaagatcgca
     4021 gacttcgcca tcaatacgag cacggatgta accctggctt gccaggttct ccagcgtttt
     4081 ggtgtgttcg cctttgcgct ctttaatgat tggcgcgagc agcatcagac gcttgccttc
     4141 cggctgcgac agcacgttat ccaccatctg gctgacggtt tgcgccgcca gcgggacgtc
     4201 gtggtccgga cagcgcggtt cgccgacgcg ggcgaacaac aaacgcaaat agtcgtggat
     4261 ttcggtgatt gtccccaccg tagaacgcgg gttatgagac gtcgatttct gctcaattga
     4321 gatggcagga gaaagcccct caatatgatc gacgtccggc ttttccatca gtgacagaaa
     4381 ctgccgcgcg taggcggaaa gggattcaac gtaacggcgc tgcccttcgg catataaggt
     4441 gtcgaaagcg agcgaggatt tgccagaacc cgaaagcccg gtcacgacaa tgagcttgtc
     4501 gcgggggata acgaggttga tgtttttgag attatgggtg cgggcgcccc gaacttcgat
     4561 cttatccatt cacctttccc ggattaaacg cttttttgcc cggtggcatg gtgctaccgg
     4621 cgatcacaaa cggttaatta tgacacaaat tgacctgaat gaatatacag tattggaatg
     4681 cattacccgg agtgttgtgt aacaatgtct ggccaggttt gtttcccgga accgaggtca
     4741 caacatagta aaagcgctat tggtaatggt acaatcgcgc gtttacactt attcagaacg
     4801 atttttttca ggagacacga acatggccag cagaggcgta aacaaggtta ttctcgttgg
     4861 taatctgggt caggacccgg aagtacgcta catgccaaat ggtggcgcag ttgccaacat
     4921 tacgctggct acttccgaat cctggcgtga taaagcgacc ggcgagatga aagaacagac
     4981 tgaatggcac cgcgttgtgc tgttcggcaa actggcagaa gtggccagcg aatatctgcg
     5041 taaaggttct caggtttata tcgaaggtca gctgcgtacc cgtaaatgga ccgatcaatc
     5101 cggtcaggat cgctacacca cagaagtcgt ggtgaacgtt ggcggcacca tgcagatgct
     5161 gggtggtcgt cagggtggtg gcgctccggc aggtggcaat atcggtggtg gtcagccgca
     5221 gggcggttgg ggtcagcctc agcagccgca gggtggcaat cagttcagcg gcggcgcgca
     5281 gtctcgcccg cagcagtccg ctccggcagc gccgtctaac gagccgccga tggactttga
     5341 tgatgacatt ccgttctgat ttgtcattaa aacaataggt tatattgttt taaggtggat
     5401 gattaaagca tctgccagcc ataaaaaaga agcctccgtt atggaggctt ctacgtatca
     5461 ggtcaaaatc attggccatt gtggcggtgt ctgattcatc tcttctatca tcaccgactc
     5521 gatgttgtgc cagatagcgg tgatgtccgc cattgtgatg ccaagcaacc ccattgcgaa
     5581 ccaacaggcg gaaacaacgc ccagaccgct gctgatcacc gcaagcccga cataatcaga
     5641 ccgacgaaaa cggatgttga gtgtgctggc cagaaacatc attacggcac taagaagttg
     5701 ccagcgaaga agaaccacgc cagtggtgag ggtagccatc aaacaattcc tctgaaaaga
     5761 gccgatgccc tggacagcgc ggcgttgttc acgggaggcg tggtacactc tggctatcgc
     5821 ggggcttgca gaacacaaaa atgaaacact ctgtttgttt cattaatttt gtgaactata
     5881 tcacaattga ttgtttgtta gccatattag gccgtgactt ttattgctgt acagattatg
     5941 tggtttttca gtggcattaa gggcatatct tcccgccgcc tctgcattcc tgtaggaaat
     6001 taattttgaa tatcaatgaa ttattttcat ccaggtgacg attagaaagg tatcaatttc
     6061 aaatcaggca aaagtgctat ttataccgta agatttatct aaagacgtcg gtacccaggg
     6121 ttttcacctt gcaatggccg ggtataaaca ggcaggaaat tgatagcaat gagtcatcgt
     6181 gcacgacacc aattactggc gttgccgggc attatctttt tagttctctt tcccatcatt
     6241 ctttcgctat ggattgcctt cctttgggca aaatcagaag tgaataatca gctccgaacc
     6301 tttgctcaac tggcactgga taaatccgag ctggtcattc gccaggcaga tttagtgagc
     6361 gatgcagctg aacgctatca ggggcaagtt tgcactccag cccatcaaaa gcgaatgttg
     6421 aatattattc gtggctatct ttatattaat gaattgatct atgcccgtga taaccatttt
     6481 ttatgctcat cgctgatagc gcctgtaaac ggctatacga ttgcaccggc cgattataag
     6541 cgtgaaccta acgtttctat ctattattac cgcgatacgc cttttttctc tggctataaa
     6601 atgacctata tgcagcgggg aaattatgtg gcggttatca accctctctt ctggagtgaa
     6661 gtgatgtctg atgacccgac attgcaatgg ggtgtgtatg atacggtgac gaaaaccttt
     6721 ttctcgttaa gcaaagaggc ctcggcagca acgttttctc cgctgattca tttgaaggat
     6781 ttaaccgtac aaagaaatgg ctatttatat gcgacagttt attcgacaaa acgcccaatt
     6841 gcagccattg ttgcgacttc atatcaacgt cttataaccc atttttataa tcatcttatt
     6901 tttgcgttgc ccgccggtat tttggggagt cttgttctgc tattactctg gctacgtatt
     6961 cgacaaaact atttatctcc caaacgtaaa ttgcaacgcg ccctcgaaaa acatcaactt
     7021 tgtctttatt accagccaat aatcgatatc aaaacagaaa aatgtatcgg cgctgaagcg
     7081 ttgttacgtt ggcttggtga gcaggggcaa ataatgaatc cggcagagtt tattccgctg
     7141 gcagaaaagg aggggatgat agaacaggta actgattatg ttattgataa tgtcttccgc
     7201 gatctgggcg attacctggc aacacatgca gatcgctatg tttctattaa cctgtcggcc
     7261 tccgattttc atacgtcacg gttgatagcg cgaatcaatc agaaaacaga gcaatacgcg
     7321 gtgcgtccgc agcaaattaa atttgaagtg actgaacatg catttcttga tgttgacaaa
     7381 atgacgccga ttattctggc tttccgccag gcaggttacg aagtggcaat tgatgatttt
     7441 ggtattggct actctaactt gcataacctt aaatcattga atgtcgatat tttgaaaatc
     7501 gacaaatcgt ttgttgaaac gctgaccacc cacaaaacca gtcatttgat tgcggaacac
     7561 atcatcgagc tggcgcacag cctggggtta aaaacgatcg ctgaaggcgt cgaaactgag
     7621 gagcaggtta actggctgcg caaacgcggc gtgcgctatt gccagggatg gttctttgcg
     7681 aaggcgatgc cgccgcaggt gtttatgcaa tggatggagc aattacccgc gcgggagtta
     7741 acgcgcgggc aataaaatta caggcggtgg cgataatcgc tgggagtgcg atcaaactgc
     7801 cgacggaaaa cgcgggagaa ggtctgctgc gagacataac ccaggtccat tgcgatatca
     7861 aaaatcggac gctcggtggt gcgcaactca acggcggcca gtaacaggcg gcgttggcga
     7921 atgtaatcgc caagcgtctg atgcgtcacc gtgcggaaca ttcgttgcaa gtaccacttt
     7981 gaatagcctg atttttttgc gactacatca atgttaagcg gctggtcaat atgctcgtca
     8041 atccatgcga taagatcctg aataattttc tgatgggaca taaatctgcc tcttttcagt
     8101 gttcagttcg ttaattcatc tgttggggag tataattcct caagttaact tgaggtaaag
     8161 cgatttatgg aaaagaaatt accccgcatt aaagcgctgc taacccccgg cgaagtggcg
     8221 aaacgcagcg gtgtggcggt atcggcgctg catttctatg aaagtaaagg gttgattacc
     8281 agtatccgta acagcggcaa tcagcggcga tataaacgtg atgtgttgcg atatgttgca
     8341 attatcaaaa ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc
     8401 gtgttgcccg aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg
     8461 cgagaagagt tggatcggcg cattcatacc ttagtggcgc tgcgtgacga actggacgga
     8521 tgtattggtt gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc
     8581 ttaggagaag aaggtaccgg cgcacgcttg ctggaagatg aacaaaacta aagcgccaca
     8641 agggcgcttt agtttgtttt ccggtctttg tctttctctc tatcccgctg gtacacagga
     8701 gggtttcccc cgacgtcaac acacctcatt cgagcacgtg gtggaggttc cggttggtgt
     8761 tgatgcttta attgtatgtc accgacgttt cttcgccagt gtaaaagtat actttttaac
     8821 cgcaatattt ttgtcatctc agacgatttt ttatcgcaat cctgaacggt atacggctcg
     8881 ataacgctgc aatcttgcgc accgacgata acgtttgcgc atcaattgcc tggtttttca
     8941 tcgtcaagac aataaaagag aaaaaagcag caaacttcgg ttgaaaaagc cgctatgatc
     9001 gccggataat cgtttgcttt ttttaccacc cgttttgtat gcgcggagct aaacgtttgc
     9061 ttttttgcga cgcagtaaat tgtcgcaaac ctggagcagg aagataacgt ttcgctggca
     9121 ggggattgtc cgccacgcat cttgacgaaa attaaactct caggggatgt tttcttatgt
     9181 ctacgccatc agcgcgtacc ggcggttcac tcgacgcctg gtttaaaatt tcacaacgtg
     9241 gaagcactgt ccgtcaggaa gtggttgccg ggttaacaac gtttctggcg atggtctact
     9301 cggtcatcgt cgttccaggt atgttgggta aagcaggctt cccgcctgcg gcagttttcg
     9361 ttgcaacctg tctggttgcc ggactcggtt ctatcgtgat gggtctgtgg gctaatctgc
     9421 cgttggcgat tggttgcgcc atctccctga cggcgtttac cgcattcagc ctggtgctgg
     9481 ggcaacatat tagcgtacct gtcgcgctgg gtgccgtgtt cctgatgggg gtgctgttta
     9541 cggtaatttc tgccacgggt atccgtagct ggattttgcg caacttgccg cacggtgtgg
     9601 cgcacggcac ggggatcggt atcggtctgt tcctgctgct cattgccgct aatggtgtcg
     9661 gtctggtgat taaaaacccg cttgatggtc tgcccgttgc gctgggtgat ttcgcgacct
     9721 tcccggtgat tatgtcactg gtaggtctgg cggtgatcat cggcctggaa aaactgaaag
     9781 tccctggtgg cattctgctg accattatcg gtatctcaat tgtcggtttg atcttcgatc
     9841 ctaacgtcca tttctccggc gttttcgcca tgccttcatt gagcgatgaa aacggcaatt
     9901 cactgattgg cagcctggac attatgggcg cgctgaatcc tgtagtcctg ccaagcgttc
     9961 tggcgctggt gatgacggca gtatttgatg ccaccggaac tatccgtgcc gtcgccggcc
    10021 aggcgaacct gctggataaa gatgggcaga tcatcgacgg tgggaaagca ctgaccactg
    10081 actccatgag cagcgttttc tctggcctgg tgggtgctgc tccggcagcg gtatacatcg
    10141 agtctgcggc gggtacggcg gcgggcggta aaaccggttt gacggctatc accgttggcg
    10201 tgctgttcct gctgattctg ttcctctctc cgctctctta cctcgttccg gggtatgcaa
    10261 cggctccggc gctgatgtac gttggcctgc tgatgctgag caacgtggcg aaaatcgact
    10321 ttgctgattt tgttgatgcg atggcgggtc tggttacggc ggtattcatc gtgctgacct
    10381 gtaacatcgt aacaggcatc atgatcggct tcgcgactct ggtgattggt cgtctggttt
    10441 ccggcgaatg gcgcaagttg aacatcggta cggtcgttat cgccgtggcg ctggtgacct
    10501 tctatgcggg tggctgggct atctaatctt tcatcggatt ctgaaaacgg gtggcaatgg
    10561 ctgcccgttt ttattttctc cgcacattgt gtgagctttt gcgatattct gaaaaaatga
    10621 gaattcaggc ataacgtctt tctcagatca taaaaaaaca tcgcaaacag ggaacgcatg
    10681 gaaatcttct tcaccatact gataatgacc ctcgtggtct cgctgtccgg ggtggtcact
    10741 cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg tgcgctactg
    10801 gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt agtcttgttt
    10861 atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt tctcgaacat
    10921 ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt cggcattggc
    10981 ttccttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc gctggcggcg
    11041 gtactttctc cgaccgatgc tgtggcgctc tccgggattg ttggcgaagg gcgcatcccg
    11101 aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcctc cggcctggtg
    11161 tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcaccgt cggcggtgcg
    11221 acggtcgaat ttatgaaagt agccattggt ggtattcttg ccggttttgt ggtgagctgg
    11281 ctgtacggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc cgcgacgcag
    11341 atcgttctgc tgttcttgct gccattcgct tcttatctga ttgccgaaca tattggcgtt
    11401 tccggcatcc tcgctgcggt tgccgccggg atgaccatca cccgctccgg tgtgatgcgc
    11461 cgtgcgccgc tggcaatgcg cctgcgcgca aacagcacct gggcgatgct ggaatttgtc
    11521 tttaacggca tggtgttcct gctgttaggt ctgcagctgc cgggtattct ggagacgtcg
    11581 ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac cgatattatt
    11641 ctgatatatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat gaaaaagttc
    11701 agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac acgagaaatc
    11761 ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc tggccggtgt gctctctatc
    11821 ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt gttcctggcg
    11881 gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat tctgctacaa
    11941 cacattgaag tcgcggatca ttcgcaacaa ttgaaagagg aacgtattgc gcgagcggca
    12001 acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc ggcggatacc
    12061 gaagagaata tcgataacca gctgcttacg gaggtcagtt ctcgcgtcat tggtaacctg
    12121 cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga gaaccttgag
    12181 cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca cctgcgcgcc
    12241 acgcgggaga tcagcaacga aacgctgcaa aaattactgc acgatctcga tttgcttgaa
    12301 gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg taaccaggcc cgaggtaatc
    12361 tgaaaaaggt gcaatctttt gcacctttaa caggttagct cgggctaaca ttcatgattc
    12421 taaaacaaat aagttatctc tcttgtaaag tcaattcatt ggctgttctt cgatttaacc
    12481 tcaatctgcg tgttgcataa tatctgcagt aatcttcata gcgactgcat taatattatc
    12541 ctcagtttgt gacatgccag tatgagaggt acaatcttct tcaaagaatt tctgtcgtgt
    12601 taccagggta tcactatcaa tggggatatt ttcctcccca cggagtatgg aaatgcccat
    12661 tccctggacg ctagcctcat caaaattaac ccgatagaga cgggtattaa acatacaggc
    12721 ttttgttaaa atagaacagt tgaaatggca gttgcttaaa ttggcattat taaaattaac
    12781 attagccata ttagagtcgg tacatataac catttcgagg aatgtatttg ttagccacgt
    12841 acaaaagagt ttgctatcaa ttaagtttat attctggagg attgctttgt tcagattgac
    12901 gcgagacaga tcagcatgtt caaagttaat taaatcgagt ttagagaaac taaggtcggc
    12961 tttaaataaa ttaacatatg gcgctattac tttataaaga tttacctcag ccatgtacgc
    13021 catataaaaa tttgaatagt caagaaaggt atttttaata ttggctctat tcattgctgc
    13081 attgtagaat tgaacatgca tagcggagca tgcatttaga gttccgtcgg ccatttttat
    13141 ctgagcgaaa tttgagtcat cgagaataca atcatcaaaa agtatttttt ccattatggc
    13201 gtaataaaaa
//
LOCUS       DAZFFI010000123        13069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFFI010000123 DAZFFI010000000
VERSION     DAZFFI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.123"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..1307
                     /gene="livK"
                     /locus_tag="ACHJ12_004106"
     CDS             198..1307
                     /gene="livK"
                     /locus_tag="ACHJ12_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4991816.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1355..2281
                     /gene="livH"
                     /locus_tag="ACHJ12_004107"
     CDS             1355..2281
                     /gene="livH"
                     /locus_tag="ACHJ12_004107"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4991817.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILMLLVMPTGILGRPEVEKV"
     gene            2278..3555
                     /gene="livM"
                     /locus_tag="ACHJ12_004108"
     CDS             2278..3555
                     /gene="livM"
                     /locus_tag="ACHJ12_004108"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4991818.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3552..4319
                     /gene="livG"
                     /locus_tag="ACHJ12_004109"
     CDS             3552..4319
                     /gene="livG"
                     /locus_tag="ACHJ12_004109"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4991819.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4321..5034
                     /gene="livF"
                     /locus_tag="ACHJ12_004110"
     CDS             4321..5034
                     /gene="livF"
                     /locus_tag="ACHJ12_004110"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4991820.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5431..6747
                     /gene="ugpB"
                     /locus_tag="ACHJ12_004111"
     CDS             5431..6747
                     /gene="ugpB"
                     /locus_tag="ACHJ12_004111"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4991821.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6845..7732
                     /gene="ugpA"
                     /locus_tag="ACHJ12_004112"
     CDS             6845..7732
                     /gene="ugpA"
                     /locus_tag="ACHJ12_004112"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4991822.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7729..8574
                     /gene="ugpE"
                     /locus_tag="ACHJ12_004113"
     CDS             7729..8574
                     /gene="ugpE"
                     /locus_tag="ACHJ12_004113"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4991823.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8576..9646
                     /gene="ugpC"
                     /locus_tag="ACHJ12_004114"
     CDS             8576..9646
                     /gene="ugpC"
                     /locus_tag="ACHJ12_004114"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4991824.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9643..10386
                     /gene="ugpQ"
                     /locus_tag="ACHJ12_004115"
     CDS             9643..10386
                     /gene="ugpQ"
                     /locus_tag="ACHJ12_004115"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4991825.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10373..10813)
                     /locus_tag="ACHJ12_004116"
     CDS             complement(10373..10813)
                     /locus_tag="ACHJ12_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4991826.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10933..12675
                     /gene="ggt"
                     /locus_tag="ACHJ12_004117"
     CDS             10933..12675
                     /gene="ggt"
                     /locus_tag="ACHJ12_004117"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4991827.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12713..12997)
                     /locus_tag="ACHJ12_004118"
     CDS             complement(12713..12997)
                     /locus_tag="ACHJ12_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO4991828.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2900 a         3326 c         3744 g         3099 t
ORIGIN      
        1 ttatctatca ataaattcag aatattatct gttcttaatc gactgaaaaa tagggatttt
       61 aatcgctatt atcacaaaat actgcgctaa acccttaatc agacaggcaa aaacagtgca
      121 gtataaaaaa agaacagtct gatttgttaa cacataaaaa caaagcaaca caacatcacg
      181 aatggggatt tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac
      241 tggcaatttc acacaccgct atggctgacg atattaaagt cgccgttgtc ggcgcgatgt
      301 ccggcccgat tgcccagtgg ggtgatatgg aatttaacgg cgcgcgtcag gcgattaaag
      361 acattaatgc caaaggggga attaagggcg acaagctggt tggcgtggaa tatgacgacg
      421 cctgcgaccc gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat
      481 acgttattgg tcatctgtgt tcttcttcta cccagcctgc gtcagatatc tatgaagacg
      541 aaggtattct gatgatctcg ccgggagcga ccaacccgga gctgacccaa cgcggttatc
      601 aacacattat gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca
      661 ttcttgagac ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa caatatggcg
      721 aagggctggc acgttcggtg caggacgggc tgaaagcggc taacgccaat gttgttttct
      781 tcgacggcat taccgctggc gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag
      841 aaaacatcga cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc
      901 aggcccgttc cgttggcctg aaaacccagt ttatggggcc ggaaggtgtg ggtaatgcgt
      961 cgctgtcgaa tattgctggc gatgctgccg aaggcatgtt ggtcaccatg ccaaaacgct
     1021 atgaccagga tccggcaaac cagggcatcg ttgatgcgct gaaagcagac aagaaagatc
     1081 cgtccgggcc ttatgtctgg atcacttacg cggcggtgca atctctggca acagcccttg
     1141 agcgtaccgg cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa
     1201 acaccgtgat tgggccgctg aactgggatg aaaaaggcga tcttaaggga tttgattttg
     1261 gtgtcttcca gtggcacgcc gacggttcat ccacggcagc caagtgatca tcccaccgcc
     1321 cgtgaaaaac gggcgggttt agaaaggtta ccttatgtct gagcagtttt tatatttctt
     1381 gcagcagatg tttaacggcg tcacgctggg cagtacctac gcgctgattg ccatcggcta
     1441 caccatggtt tacggcatta tcggcatgat caacttcgcc cacggcgagg tttatatgat
     1501 cggcagctat gtctcattta tgatcatcgc cgcgctgatg atgatgggca ttgataccgg
     1561 ctggctgctg gtggcggcgg gattcgtcgg cgcaatcgtc atcgccagcg cctacggctg
     1621 gagtatcgaa cgggtggcct atcgcccggt gcgcaactcc aagcgcctga ttgcgctcat
     1681 ctcggcaatc ggtatgtcca tcttcctgca aaactatgtc agcctgaccg aaggttcgcg
     1741 cgacgtggcg ctgccgagcc tgtttaacgg gcagtgggtg gtgggccata gcgaaaactt
     1801 ctctgcctct attaccacca tgcaggcggt gatctggatt gttactttcc tcgccatgct
     1861 ggcgctgacg attttcattc gctattcccg tatggggcgc gcctgtcgtg cctgcgcgga
     1921 agatctgaaa atggcgagcc tgcttggcat caacaccgac cgggtgattg cgctgacctt
     1981 tgtgattggc gcggcaatgg cggcggtggc cggtgtactg ctcggtcagt tctacggtgt
     2041 gattaacccc tacatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg
     2101 tgggattggc agtattccgg gggcgatgat tggcgggctg attctgggga ttgcggaggc
     2161 gctctcttct gcctatctga gtacggaata taaggatgtg gtttcatttg ccctgctgat
     2221 tctgatgctg ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg
     2281 aaaccgatgc atattgcaat ggcgttgctc tctgccgcga tgttttttgt cctggcgggc
     2341 gtctttatgg gcgtacaact ggagctggat ggcaccaaac tggtggtcga cagcgcttcg
     2401 gatgtccgtt ggcagtgggt gtttatcggc acggcggtgg tctttttctt tcagcttttg
     2461 cgacctgctt tccagaaagg gttgaaaagt gtttccggac cgaagtttat tctgcccgcg
     2521 attgatggct ccacggtgaa gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg
     2581 gcgtggccgt ttatggtgtc acgtgggacg gtggatatcg ccactctgac catgatctac
     2641 attatcctcg gtctcgggct gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg
     2701 tacggcggct tttacgccat cggcgcttac acttttgcgc tgctcaatca ctattacggc
     2761 ctgggcttct ggacctgtct gccgattgcc gggttaatgg ccgcggcggc gggcttcctg
     2821 ctcggctttc cggtgcttcg cttgcgcggt gactatctgg caatcgttac cctcggtttc
     2881 ggcgaaattg tgcgcatatt gttgctcaat aataccgaaa ttaccggcgg cccgaacgga
     2941 atcagtcaga tcccgaaacc gacactcttc ggactcgagt tcagccgtac cgctcgtgaa
     3001 ggcggctggg acacgttcag taatttcttt ggcctgaaat acgatccctc cgatcgtgtc
     3061 atcttcctct acctggtggc gttgctgctg gtggtattaa gtctgtttgt cattaaccgc
     3121 ctgctgcgga tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagacga gatcgcctgc
     3181 cgttcgctgg gtttaagccc gcgtcgtatc aagctgaccg cttttaccat cagtgccgcg
     3241 tttgccggtt ttgccggaac gctgtttgct gcgcgtcagg gctttgtcag cccggaatcc
     3301 ttcacctttg ccgaatcggc gtttgtgctg gcgatagtgg tgctcggcgg tatgggctcg
     3361 caatttgcgg tgattctggc ggcaattttg ctggtggtgt cgcgcgagtt gatgcgtgat
     3421 ttcaacgaat acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt
     3481 ccgcagggct tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa
     3541 ggagagcagg catgagtcag ccattattat ctgttaacgg tctgatgatg cgcttcggcg
     3601 gcttgctggc ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa
     3661 tcggccctaa cggtgccgga aaaaccacgg tttttaactg cctgaccgga ttctacaaac
     3721 ccaccggcgg cactatttta ctgcgtgatc agcatctgga aggtttaccg gggcagcaaa
     3781 ttgcccgcat gggcgtggtg cgtaccttcc agcatgtgcg tctgttccgt gaaatgacgg
     3841 taattgaaaa cctgctggtg gcgcagcatc agcaactgaa aaccgggctg ttctctggcc
     3901 tgttgaaaac gccatctttc cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct
     3961 ggcttgagcg cattggtttg ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg
     4021 gtgaccaacg ccgtctggag attgcccgct gcatggtgac gcagccggag attttaatgc
     4081 tcgacgaacc ggcggcaggt cttaacccga aagagacgaa agagctggat gagctgattg
     4141 ccgaactgcg caatcatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg
     4201 tgatgggaat ttcggaccga atttacgtgg tcaatcaggg gacgccactg gcaaacggta
     4261 cgccggagca aatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag
     4321 atggaaaaag tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg
     4381 ctgcatgagg tgagcctgca tatcaatcag ggcgagattg tcaccctcat tggcgcgaac
     4441 ggggcgggga aaaccacctt gctcggcacg ttatgcggcg atccgcgtgc caccagcggg
     4501 cgaattgtgt ttgatgataa agacattacc gactggcaaa cggcgaaaat catgcgcgaa
     4561 gcggtagcga ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac
     4621 ctggcgatgg gcggcttttt tgccgaacgt gatcagtttc aggagcgcat aaagtgggtg
     4681 tatgagctgt ttccacgtct ccatgagcgc cgtgttcaac gggcgggcac catgtccggc
     4741 ggtgaacagc agatgctggc gattggtcgc gcgctgatga gcaacccgcg tttgctgcta
     4801 cttgatgagc catcgctcgg tcttgcgccg attatcatcc agcaaatttt cgacaccatc
     4861 gagcagctgc gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc caaccaggcg
     4921 ctaaagctgg cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct ctccgatact
     4981 ggtgatgcac tactggcgaa cgaagcggtg agaagtgcgt atttaggcgg gtaataacac
     5041 gttgattgat aaggagtcaa aagacttctt tgagacaggt gacaaatgta aaattgcctg
     5101 atgcgctgcg cttatcaggc ctactgggtg ggttgcaata tattgaattt gcacgatctt
     5161 gtaggccgga taaggcgttt acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt
     5221 ttgattcaga ccctctattt ttccgcttat ccgtgcccca tctcccattt tccctcaccc
     5281 acgccgtcac cgccttgtca tctttctgac accttactat cttacaaatg taacaaaaaa
     5341 gttatttttc tgtaattcga gcatgtcatg ttaccccgcg agcataaaac gcgtgaattc
     5401 gcgcattcgg tacaacaaga gagataaacg atgaaaccgt tacattatac agcttcagca
     5461 ctggcgctcg gactggcgtt aatggggaat gcacaggcag tgacgaccat tccgttctgg
     5521 cattctatgg aaggggaact gggtaaagag gtggattctc tggcccaacg ttttaacgcc
     5581 gaaaatccgg attacaaaat tgtaccgacc tataaaggca actacgaaca gaatttaagc
     5641 gcggggattg ccgcatttcg taccggcaac gctccggcta ttttgcaggt ttatgaagtt
     5701 ggcaccgcca ccatgatggc atcgaaagcc attaaaccgg tatatgacgt gtttaaagag
     5761 gcggggattc aattcgatga gtcgcagttt gtgccgacgg tttccggtta ctactccgac
     5821 agcaaaacgg gccacttact ctcccagcca ttcaacagct cgacccccgt tctctattac
     5881 aacaaagacg ccttcaagaa agcaggatta gacccggaac aaccgccgaa aacctggcag
     5941 gatctggcgg actatgccgc gaaactgaaa gcctccggta tgaagtgcgg ctacgccagc
     6001 ggctggcagg gctggatcca actggaaaac tttagcgcct ggaacggtct gccgtttgcc
     6061 agcaaaaaca acggctttga cggcacggac gcggtgctgg agttcaacaa gccggagcag
     6121 gtgaaacaca tcgccatgct cgaggagatg aacaagaagg gcgacttcag ctacgtcggt
     6181 cgtaaggatg aatccaccga gaagttctat aacggtgatt gcgcgatgac caccgcctct
     6241 tccggttctc tcgctaatat tcgtgagtac gccaaattta actatggcgt aggcatgatg
     6301 ccttacgacg ccgatgcgaa agatgcaccg caaaacgcca ttatcggcgg ggccagcctg
     6361 tgggtgatgc agggcaaaaa taaagaaacg tataccggcg tggcgaagtt cctcgacttc
     6421 ctcgcgaagc cagaaaacgc tgccgagtgg catcagaaaa ccggttatct gccaatcacc
     6481 aaagcagcgt atgacctgac ccgtgagcag ggcttttacg agaaaaaccc aggggcggat
     6541 accgcgacgc gtcagatgct gaataagccg ccgttgccgt tcaccaaagg gctgcgtctg
     6601 ggcaacatgc cgcagatccg cgtgattgtg gatgaagagc tggagagcgt gtggaccggt
     6661 aagaagacac cacagcaggc actggatacc gccgttgagc gtggaaatca gttgctgcgc
     6721 cgctttgaga aatcgacgaa gtcttaatca gtgtaatgtc ggatgcgttt cgcttatctg
     6781 acctggcatc gcgtgtaggc cggataagcg aagcgcatcc ggcacagttc aggaattaac
     6841 cgtaatgtca tcatcccgtc cggtgttccg ctcgcgctgg ctgccttatc tgctggtcgc
     6901 gccgcagctc atcatcaccg ttatcttttt tatctggcct gcgggcgaag cgttgtggta
     6961 ctcgctacaa agcgtcgatc cgtttggttt ctccagccag tttgtcggcc tggataactt
     7021 cgtcacgctg ttccatgaca gctactatct cgactccttc tggacgacga taaaattcag
     7081 cacctttgtc accgtcagcg gtttgctggt atcgctgttc tttgcggcgc tggtggagta
     7141 catcgtgcgc ggcagccgtt tctatcaaac cttaatgttg ctgccttacg ccgtggctcc
     7201 cgccgttgcc gccgtattgt ggatcttcct gtttaacccc ggtcgcgggc tgatcaccca
     7261 ttttctcgcg gagttcggct acgactggaa ccacgcgcaa aacagcggtc aggcaatgtt
     7321 tctggtggtg tttgcctcag tatggaagca aatcagctac aacttcctgt tcttctatgc
     7381 cgcgctgcaa tccattcccc gttcgttgat cgaagccgca gccatcgacg gtgcagggcc
     7441 gattcgccgc ttctttaaga ttgcgctgcc gcttatcgcc ccggtgagtt tcttcctgct
     7501 ggtagtgaac ctggtgtatg ccttcttcga caccttcccg gtgatcgacg ccgccacgtc
     7561 cggcgggccg gttcaggcca ccacgacgct gatttataag atctaccgcg aaggttttac
     7621 cggactggat ctggcttcgt ctgccgcaca gtcggtggtg ttgatgttcc tcgtcatcgt
     7681 gctgacggtg gtgcagttcc gctatgttga aagcaaggtg cgttaccaat gattgagaac
     7741 cgtccgtggc tgacgatatt cagccatacc atgctgatcc tcgggatcgc ggtgatcctc
     7801 ttcccgctgt acgtggcgtt tgtcgcggcg acgctggata aacaggccgt ctatgccgcg
     7861 ccgatgacgc tcatccccgg cacacatctg ctggaaaaca tccacaacat ctgggtgaac
     7921 ggggtaggca cgaatagcgc gccgttctgg cggatgttgc ttaacagctt tgtgatggcg
     7981 ttcagcatta cgctcggcaa aattaccgtc tcgatgctct cggcatttgc cattgtctgg
     8041 tttcgttttc cgctacgtaa cctcttcttc tggatgattt ttatcaccct gatgctgccg
     8101 gttgaagtac gtatcttccc gacggtggaa gtcatcgcca acctgcagat gctcgacagc
     8161 tacgccggtt taacgctgcc gctgatggcc tcggcgaccg ctactttcct gttccgccag
     8221 ttctttatga cgctgccgga tgagctggtg gaagccgcgc ggatcgacgg cgcatcgcca
     8281 atgcgcttct tttgcgacat cgtttttccg ctctccaaaa ctaatctggc ggcgctgttt
     8341 gtgatcacct ttatctacgg ctggaatcag tatttgtggc cgttgttgat tattaccgat
     8401 gtggatctcg gcaccaccgt ggcagggatc aaagggatga tcgctacagg cgaaggcacc
     8461 acggaatgga actcagtgat ggcggcgatg ttgttaacgc ttatccctcc ggtggtgatt
     8521 gttttagtga tgcagcgtgc cttcgtgcgc ggcctggtcg atagtgagaa ataagatggc
     8581 aggactgaaa ttacaggcag taaccaaaag ctgggatggt aaaacccagg tcattaaacc
     8641 gctgaccctt gatgtggcgg atggcgaatt tatcgtgatg gtcgggccgt ctggctgcgg
     8701 gaaatcgacg ctgctgcgca tggttgccgg gctggagcgg gtgacagaag gcgatatctg
     8761 gatcaacgac cagcgcgtga ctgaaatgga gccaaaagat cgcgggattg cgatggtgtt
     8821 ccagaactac gcgctttatc cgcatatgag tgtcgaagaa aacatggcgt gggggctgaa
     8881 aattcgcggc atgggcaagc agcaaattgc cgagcgcgtt aaagaagcgg cgcgcattct
     8941 ggagctggac ggtctgctca aacgtcgccc gcgcgagctt tccggcggtc agcgccagcg
     9001 tgtggcgatg ggccgcgcga ttgtgcgcga tccggcggtg ttcctgtttg atgagccgct
     9061 ctctaacctc gatgccaagc tgcgcgtgca gatgcgtctt gaactgcaac agttgcaccg
     9121 tcgcctgaaa acgacttcac tctacgttac tcacgatcag gttgaagcga tgacgctcgc
     9181 ccagcgagta atggtgatga acggcggcgt tgccgaacag attggcacac cagttgaagt
     9241 ctacgaaaag cccgccagcc tgtttgtagc gagttttatc ggcagtccgg cgatgaacct
     9301 gctgacaggc cgcgtgaata ccgaaggcac gcatttcgaa ctggacggcg gtatcgtgct
     9361 accgctaaac ggtggctacc gtcagtatgc cgggcgtaaa atgactctcg gcattcgccc
     9421 ggaacatatt gcgctaagct cgcaagcaga aggtggcgta ccgctggtga tggatacgct
     9481 ggagatcctc ggcgcagata acttggcgca cggacgctgg ggcgagcaga agctggtggt
     9541 gcgactggcg catcaggagc gcccgacggc aggcagcacg ctgtggctgc atctggcgga
     9601 aaatcagctg catctttttg atggtgaaac aggacaacga gtatgagtaa ctggccttat
     9661 ccccgcatcg tcgctcatcg tggcggcggt aagctggccc ccgaaaacac cctggcggca
     9721 atcgacgttg gggcaaaata cggtcataag atgatcgaat ttgacgcgaa gttatcgaaa
     9781 gatggcgaga tcttcctgct ccatgacgac aatctcgaac gcaccagcaa cggctggggc
     9841 gtcgcgggtg aactgaactg gcaggattta ctgcgcgtgg atgcgggcag ttggtacagc
     9901 aaagcgttta aaggtgagcc gctgccgttg ctttcgcagg tggcggaacg ctgtcgcgaa
     9961 cacgggatga tggcgaatat cgaaatcaaa cccaccactg gcactggacc attaacgggc
    10021 aaaatggtgg cgctggcggc acgccaactg tgggccggta tgacgccgcc gctgctgtca
    10081 tcgtttgaga ttgatgcttt agaagctgca cagctggcgg caccggaact gccgcgtggt
    10141 ttattgcttg atgagtggcg cgacgactgg cgcgaactga ccgcgcggct gggctgcgtc
    10201 tctattcatc tcaatcataa gttactcgat aaagtgcgag tgatgcagtt gaaagacgcc
    10261 ggactgcgga ttctggttta taccgtcaat aaaccccagc gcgcggcaga gttgctgcgc
    10321 tggggcgtgg attgcatctg taccgatgcg attgacgtga ttggtccgaa ctttacggcc
    10381 caatagtttt caacggaatg tcaggctgcg gcgtgctggg ctgattcagc atgtcgccgt
    10441 ttctcctctc cggcagcata tgctgctgat tcaacgaact atccggatta cggttgctgt
    10501 ttaacatccc gccgttggtg ttgggcagca tttgctgccg ggcgggattt cgctcccccg
    10561 gctgtgactg caacacccgc tgagaattat tgtttatctg gttttctaaa tgctgctgtt
    10621 gcaactgagt ttgcgttttc agttgctgat tcagcatccc tttttgctgg atttgctgag
    10681 tctgcatctg ggtttgcatc cgctgctggc tgggtatctg ataccccggc tggttagggt
    10741 tgttcagagt attaatgggc tgtgcaaagc cgacaaacgg caggagtgcc gtaagaatca
    10801 gaagtcgttt catcgcgtat cctcctctga agatatcctt taagtttact cgcttcccga
    10861 caaaacgatg attaattcag agttatatac caggcttagc tggggttgcc ccttaatctc
    10921 tggagaataa cgatgataaa accgacgttt ttacgccggg tggccattgc tgctctgctc
    10981 tcaggaagtt gttttagcgc cgccgccgcg cctcctgcgc cgcccgtctc gtatggtgtg
    11041 gaggaagatg tcttccaccc ggtacgcgcg aaacagggaa tggtagcgtc tgtggacgcc
    11101 actgccactc aggtgggggt ggatattctc aaggagggcg ggaatgccgt tgatgccgcc
    11161 gtggcggtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct gggcggtggt
    11221 ggttttatgt taatccgctc gaaaaatggc aataccacgg ctatcgattt ccgcgaaatg
    11281 gcacccgcca aagcgacccg cgatatgttc ctcgatgatc agggcaaccc ggacagcaaa
    11341 aaatcactca cttcgcatct ggcttccggc acaccgggta cggtagcagg tttctcgctg
    11401 gcgctggata aatacggcac catgccgctg aacaaagtcg tgcagcccgc gtttaaactg
    11461 gcacgcgatg gttttatcgt taacgacgcg ctggctgacg atctcaaaac ctacggtagc
    11521 gaagtgttgc cgaatcacga aaacagtaaa gctatcttct ggaaagaggg cgagccgctg
    11581 aaaaagggcg acacgctggt gcaggcgaac ctggcaaaga gcctggagat gattgctgaa
    11641 aacggcccgg acgaattcta taaaggcacg attgcggaac agatcgccca ggagatgcag
    11701 aaaaacggtg gcttgatcac taaagaagat ttagcagcct ataaagcggt cgaacgcact
    11761 ccgataagcg gcgattatcg cgggtatcag gtttactcca tgccaccgcc atcctccggc
    11821 gggatccata tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa gaaatacggc
    11881 tttggcagcg ccgatgcgat gcaaatcatg gcagaagcgg agaaatacgc ctacgccgac
    11941 cgctcggaat atcttggcga cccggatttt gtcaaagtac cgtggcaggc gctgaccaat
    12001 aaagcctatg ccaaatctat tgccgatcaa attgatatca ataaagcgaa gccatccagc
    12061 gaaattcgcc ccggcaagct tgcgccttat gagagtaatc aaactaccca ttactcagtg
    12121 gtggataaag atggtaacgc ggtggcggtg acctatacgc tgaacaccac cttcggtacg
    12181 ggcattgtcg cgggcgagag cggtattctg cttaataacc agatggatga tttctccgcc
    12241 aaaccgggcg taccgaacgt ttacgggctg gtgggcggtg atgccaacgc cgtcgggccg
    12301 aacaaacgcc cgctgtcgtc gatgtcgccg accattgtgg tgaaagacgg taaaacctgg
    12361 ctggttaccg gtagcccagg cggtagccgg atcatcacta cagtgctgca aatggtggtg
    12421 aatagcatcg attatggcat gaacgtcgcc gaagcgacca atgcgccgcg tttccaccat
    12481 cagtggttgc cggacgagct gcgtgtcgaa aaagggttta gcccggatac gctcaagctg
    12541 ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac acaaagcatt
    12601 atggttgggc cggacggtga gttgtacggc gcatccgacc cgcgctcggt ggatgattta
    12661 acggcggggt actaaggtta gcggccctct tcgtgggaag agggctattt tgtcagggca
    12721 agccgaaggt agcctttttt atttcgtaat cctgtagata ttcttccagc ggctttgccg
    12781 ttcccagaga atgaatttca ccactgtctt tatcaataat aaaaggtgcg ttaccagcta
    12841 agcgcgcggc ctcatctcca gtttcgagaa attctcgtgc ttcgaaacag aaataccagc
    12901 cctggctaaa gcgtccatgt agagtaatga cgaccgggag atctgcatca tcaaggtaat
    12961 ggttcgcttt cgcgaatgcg tcgtgataag taatcataat tataataaat attattgttg
    13021 agtgttatat tattattcct gtgtgataca ttgagcaaag acgcgttca
//
LOCUS       DAZFFI010000124        12978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFFI010000124 DAZFFI010000000
VERSION     DAZFFI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.124"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(23..715)
                     /locus_tag="ACHJ12_004119"
     CDS             complement(23..715)
                     /locus_tag="ACHJ12_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO4991829.1"
                     /translation="MRLTTIPTFAVRIVVENNSLSLPSGMVSYGQAVSSWNQYADANN
                     LTSEQKQAGLDKLAKGELPEGANISKVIVDGYKDGVLIAGAWYLGPAVSVGKAAVGGV
                     TSAAVNGGFQWYGLSQPGSENQSWDYKGSASSFMTGMLAPGRTVTANTAIALGGSVFS
                     DGANIKSQSGTALGAYAGGYVGEYFPVILRPVLGNSASTVGDIGGGFVSELINYGIKN
                     YPTPTNKEPEHE"
     gene            complement(957..9902)
                     /locus_tag="ACHJ12_004120"
     CDS             complement(957..9902)
                     /locus_tag="ACHJ12_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO4991830.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGASPSSRTGYPHRQR
                     ICRVTPLAFSLWLASGMVHSVSAAGIVADHGAPGHQQPTIMQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNQGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGISCDGCGFINANRATLTTGQPQMKNGSLTGFRVEGG
                     EIQVTGKGMDASRTDYTDIIARSVKINAGVWAQDLKVTTGRNNVDIAHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLATTRELNNSGSVYAGQDTQIQSDGVFTHTGSVASRRNTRIQTTRLTGGE
                     RSLLAAGVKDDGRLAEAGNLTVSTTGELAAHGQVLSGGDMQLKGQGLDLSNSRIQGQH
                     TGLDAGSGNLSTQNAQLSAGTFAARTAGQFSNNGATINAGTLQISAQSLSNHKGSLIQ
                     TGTGDFSLSLPGSVDNREGLLAANGAVRLDALSLDNRRGKVQAAQSGSLQVKTTGAVD
                     NLQGTLIASRDILINTQALNNDNGLISAAAGTGRIKTQQAVSNTAGRMESAGRLDISA
                     GNLNNHQGTVVSDELSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSGSDMTL
                     DVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFMAGQKDVTLNTGTLDNRQGAL
                     GSQASLQVSSGTLMNQKGALKAGTDMLLSGDDVSNQEGTLAAGGDLNAHLNVLENQQG
                     TVVSNGNSRLDVTRSDNQGGRLVAQQSLTLTSKDIINDAGGLIQSGASLNIRADTLSN
                     RDSGDRGGVISQGPVTLNSGTLDSTAGVLLSGDALSLTAGVVNNTSGQVVANGLLGWN
                     SQALNNQSGLIQGWGISINTAGQTLDNRRGTLNSLQELTVSSGAMDNRGGTVGAKTTA
                     DLSTTSLDNREGGRLVSEGEIRLHTRGLQNSHGQIQSVGDMLLDSVRGVVDNVSGLIR
                     SGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQEGSILADRALTVMADRTLS
                     NNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVDAGQLGGDGKLLSPGDMTLKSN
                     TAFSNSGQTIANGNLTLSVNGDVTNTGSLLAGSRLELNSIRLENTEKGEISAGQTWLN
                     VTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYGDAVGVSAATFNNLEENGVAATLAGR
                     ERVDLGVQTLNNRTHSLIYSAGDMHIGGTLDANGSATGKAGVLNNHSATVEATGSLAL
                     SAGQINNVNDHFTTERVVVSTEKVTEYQLSGSDKRWSAGEPGVYVDNDSSNSLKKLHT
                     PEGARDKFTQYDYTRTVEETRVKDSDPGKILSGAGMTVVADKLLNDKSQVVAGGLLSI
                     PSGSVENVSVSGERHVTDSGTSTYYYRIRKKGKDKQGEKTSQYTPPTVIQTITLKPGE
                     LTSHGQVQGSHVTLSPLKPQGTDVQTGQAGNVDATVAATDRIPLRPVVSAGEPVILLP
                     GQQFEVSTPQGSIHVAGPDTRLPDSSLFKTNPAVNVPYLVETDPRFTNQKMWLGSDYM
                     QKAFSQNGDNMLKRLGDGFYEQRIIREQVVALTGQRFLDGYGSDEEQFKALMDAGIAF
                     GKQYNLKPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQVYARVKPGDVNS
                     AGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGSIQGANVSLTARTDIN
                     STGGLLQATDSLLAMAGRDISLTTTTRTAQSDAGQNHFERTSIDSVAGVYVQNDQGRL
                     VLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMTLSTVTTSAQDNITWDKNNRLSQGVT
                     QSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGHDVTLTGAQNTSSLDEYHK
                     VTGSSGMLSKTTTTTHDVSDRRTMTGSELNGDTVSIGAGHNLNVTGSSVAGDNRVSLA
                     AGNNLNIGMLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVRDTATDTTQKGSTV
                     GSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGKEVSITAATDQHVQTHTVEQKTSGLT
                     LALSGTVGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQAGRLAEAQGNQK
                     ENNNAVGISLSYGSQSSKSEQQSEQTVAKGSTLTAGNNLSIQATGSGVKGVDGDLTIQ
                     GSQIKAGNNVLLQANRDVNLVSAENTSKLEGKNTSSGGSVGVGVGVGSGGWGISVSAS
                     ANQGKGSEKGNGTTHTETTVDAGNRLTIISGRDTTLTGAQAGGETVKVDAGRHLTLTS
                     EQDSDRYDSKQQNASAGGSFTFGSMSGSASVNLSRDKMHSNYDSVQEQTGIFAGRGGF
                     DVTTGQHTQLNGAVIASTATADKNRLDTGTLGFRDIENRADYKVEHQSVGISTGGSIG
                     GQFAGNMANNLLVGANHEGHAESTTQSAVSAGNITIRDTQSQKQDVADLNRDAAHANQ
                     TLSPIFDREKEQQRLQMAQLIGEIGNQVADIVRTEGQIAGEKAKRDPAALNQARAELE
                     AAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGGA
                     APYIAEIIKQTTPDGAGRVAAHAVVNAALSLAQGKNALAGAAGAATGEVVGMIATQMY
                     GKPVSELSETEKQTVSTLATVAAGLAGGLVGDSGASAVAGAQSGKTTVENNALSFGDG
                     FESNAAAATSWNKYAVDNNLTPEQTQAGLDKIAKGDMPDSTNITKVIVDGYQDGVMIA
                     GAWYLGPAASVGKMVSGGVVAEIANGSYQWFDLSQPGNENKNWDWKSSASSGIAGMLA
                     PGRTVGQNVGIAMGGAFFTDGPNTGSIGGAAAGAGLGGAFGVYAPGIVNSVTGKEFPG
                     FIFDATGSFGSEFLGGYIKEASSNNQFSSSAKKEEGK"
     gene            complement(9918..10445)
                     /locus_tag="ACHJ12_004121"
     CDS             complement(9918..10445)
                     /locus_tag="ACHJ12_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO4991831.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAVVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSLDAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGVQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            complement(10455..11534)
                     /locus_tag="ACHJ12_004122"
     CDS             complement(10455..11534)
                     /locus_tag="ACHJ12_004122"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4991832.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQMNA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            complement(11532..12107)
                     /locus_tag="ACHJ12_004123"
                     /pseudo
     CDS             complement(11532..12107)
                     /locus_tag="ACHJ12_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
BASE COUNT         2764 a         3893 c         3088 g         3233 t
ORIGIN      
        1 taataaatat actgaaggtt ttttactcat gttctggctc cttattagta ggagtaggat
       61 aatttttaat accgtagttt attaactcag aaacaaagcc accaccaata tcaccaacag
      121 tacttgcaga gttacctaac actggcctca atatcacagg gaaatattct cccacatatc
      181 cacctgcgta tgccccgaga gcagttccag attgggactt aatattagca ccatcgctaa
      241 atacagatcc ccccagcgct atagctgtat tagctgtaac tgtacgacct ggagcaagca
      301 tccctgtcat gaatgaagat gcgctacctt tataatccca tgactgattt tcactaccag
      361 gttggctcaa tccataccat tgaaaaccac cgttcacagc agcggaagta actccaccga
      421 ccgcagcttt acccactgac accgcaggcc ccagatacca ggcacctgca atcaatacgc
      481 catctttata accatcaact ataactttgc ttatattggc tccttcaggt aactcacctt
      541 tggcaagttt atccagtcct gcctgcttct gttccgaggt gagattattg gcatcagcat
      601 actgattcca tgaggaaacc gcctgaccat aactcaccat tcctgatgga agactgagcg
      661 agttattctc aacaacaatc ctaaccgcaa aggttgggat tgttgttaat ctcactcagg
      721 atctgatgga ggttttttac gagaattaaa cttatcgatc agattaaaaa ctatcgctgc
      781 tagtgttatc gatgtacctg ctatgcaccc aaacacaata actcttatca tttggtgggt
      841 cgggaaaaaa aagaatcctt ttgttaggta aataaataaa gctcctcccc ccatcaaaag
      901 aaatccactg atgatacaaa agaggcatgc atatatgaac aatacaatgt aagcaatcat
      961 ttcccctcct ccttcttcgc gctagaagag aactgattat tactacttgc ttcttttata
     1021 tatccaccaa gaaattcact accaaaagat cctgtcgcat caaatataaa tcccggaaac
     1081 tctttcccag taactgagtt tactattccg ggagcatata ctccaaatgc tccacccaat
     1141 ccggccccgg cagcagcccc tccaatagag cctgtatttg gaccatcggt aaagaatgca
     1201 ccacccatcg caattccgac gttttgtccg acagttctcc ccggcgcaag catacccgca
     1261 atgcctgaac tggcactgct tttccagtcc cagtttttgt tttcattacc tggctggctt
     1321 aaatcaaacc actggtaact tccattggct atctccgcga cgactccacc actgaccatt
     1381 ttaccgaccg aagcagcagg ccccagatac catgccccgg caatcattac accatcctga
     1441 tatccatcca cgatcacttt cgtgatattg gtgctgtccg gcatatcacc tttcgctatc
     1501 ttatccagtc cagcctgtgt ttgttccggt gtcagattat tgtccaccgc gtacttgttc
     1561 catgatgtag ccgcagcagc attgctttca aaaccatcac cgaagctgag cgcattattc
     1621 tcaaccgtcg tcttacctga ctgtgctccg gctaccgctg aagctcccga atctcccacc
     1681 agaccaccgg ccaggccggc agcaaccgtt gccagtgtcg agaccgtctg cttctctgtc
     1741 tcgctcagtt cactcaccgg cttaccgtac atctgtgtgg ctatcattcc caccacctcg
     1801 ccggtggctg cgcctgcggc accagccaga gcattcttac cctgtgccag agacagggct
     1861 gcattcacta ctgcatgagc tgccacacgt cccgcaccgt ccggggtggt ctgtttgata
     1921 atttcagcaa tatacggtgc tgcaccacct gccagggctg cactcagatt accgccggcc
     1981 agcccctgta ctgctgccgt tgcggcctgt atcgcctgct ggtatttgcc tcccgttcca
     2041 aaacctgagg ccgccatacc gttattgtag gcccgctgtg ccacatcctg ttccgtaaac
     2101 ggttttcctg ctgcctcaag ctctgcgcgg gcctgattca gtgctgccgg gtcccgcttc
     2161 gctttctcac cagctatctg gccttccgtt ctgacaatat ctgccacctg gttaccaatc
     2221 tcaccaatga gctgcgccat ctgcaaccgc tgctgctctt tttccctgtc aaatatgggg
     2281 gacagtgtct gatttgcatg tgctgcgtca cggttcaggt ctgccacatc ctgtttctgg
     2341 ctctgcgtat cccgaatggt gatattcccg gcagacacag ccgactgagt ggtgctttct
     2401 gcatggcctt catgatttgc accaaccagc agattgttgg ccatatttcc cgcgaactgg
     2461 ccaccgatgc tgccgccagt gctgataccc acgctctggt gttcaacttt gtaatcagca
     2521 cgattttcga tatcacggaa gcccagtgta ccggtatcca gccggttttt atctgccgtt
     2581 gccgtggacg caatcaccgc accgttcagt tgcgtatgct gtccggtcgt gacatcaaac
     2641 ccgcccctgc cggcaaaaat ccccgtctgc tcctgcacac tgtcgtagtt actgtgcatt
     2701 ttatcccggc tgaggttcac actcgccgaa ccgctcatgg acccgaaagt gaagctgcct
     2761 ccggcgctgg cattctgctg cttactgtca taacggtcac tgtcctgctc actggtcagt
     2821 gtcaggtggc gaccggcatc cactttcacc gtttcaccgc cagcctgcgc gccggtaagc
     2881 gtggtgtccc gcccgctgat aatggtcagc cggtttcccg catccaccgt ggtttctgta
     2941 tgggtggtac cgttgccttt ttcactgccc ttaccctggt ttgcgctggc tgaaacactg
     3001 ataccccagc ctccggagcc cacaccaaca ccgacaccca cgctgccacc actggatgtg
     3061 ttctttcctt ccagctttga agtgttttct gctgatacca gattcacgtc acggtttgcc
     3121 tgcagcagaa cgttgttccc ggcctttatc tgactgccct gtatcgtcag gtcaccatcc
     3181 acgcccttca cgccggagcc ggtggcctga atactgaggt tattacctgc ggtcagtgta
     3241 ctgcccttcg ccaccgtctg ctctgactgc tgttctgatt tcgaagactg gctgccgtaa
     3301 gagaggctga tacccacggc attgttattc tctttctggt tgccctgtgc ttctgccagt
     3361 cgtccggcct gcacggcctg agcccccgag agcgcggcct tcaccccctg cagggcttca
     3421 agccggctgt ttccggcaga tttcgccgcc tgtacggtct ccaccgtcgt gttcagggca
     3481 ctgcccactg tgccggacag ggcaagcgtc agaccggagg ttttctgctc cactgtatgc
     3541 gtctgcacat gctggtctgt ggcggcggta atgctgacct ccttaccgct gagtgccatg
     3601 tccctgccgg caatcaggtc agagccgttg acggtcaacc ggttacctgc ctgaagggaa
     3661 acatcaccgt gaacactccc caccgtgctg cctttctgtg tggtgtctgt tgcggtgtcc
     3721 ctgactttca gactctggct gcccacgctg aatcccacac ctccggaact catcagcccg
     3781 gattttttct cctgcttcag gtgtgtttca cggttgcttt ctgtcagcat accgatattc
     3841 aggttatttc cggcagccag ggagacacgg ttatccccgg ccacactgct gcccgtgacg
     3901 ttcagattat gtccggcgcc gatactgacc gtatccccgt tcagttcact gcctgtcatg
     3961 gtccggcggt cactgacatc atgcgtggtt gtggtggttt tcgataacat cccgctgctg
     4021 ccggtcactt tatggtattc gtccagcgaa ctggtattct gtgcaccggt cagcgtcacg
     4081 tcatgacccg ccatcagggc caggcctttc tgagctgaca gcgatgccgc ctgtgacgtc
     4141 atatcccgtc cggcggtcag cgtgacatca ccgttaccgg caagcgtgct gcccgtactc
     4201 tgtgtgacgc cctgcgacag acgattattt ttgtcccagg taatattgtc ctgcgcggat
     4261 gtggtcactg tggagagtgt catatccctg ccggcactga gttgcgtcag gctgtcctta
     4321 ccctgattca ccaccgttgc agccgtcaga ttcatatccc gtccggcctg cagcaccagt
     4381 cttccctggt cgttctgcac atacacgccg gccacactgt cgatactggt acgttcaaaa
     4441 tggttctgtc cggcgtcact ctgtgccgta cgagtggtgg tggtcagact gatatcccgt
     4501 ccggccatcg ccagcaggct gtccgtggcc tgcagcagcc cgccggtact gttaatatcc
     4561 gtccgggccg tcaggctgac atttgctccc tgaatggagc cggcctggtt gtgaatgttt
     4621 tctgcactga gctgcacggt ctgcttcccg gccagtttac cgctgttaaa caggtcacca
     4681 tccagcttca tcaccatgtc cctgccggca atcagcgcac cggcactgtt cacatcaccg
     4741 ggttttacac gggcatacac ctgaggtacc tgaactgtct gcgtgctgcc gtccggcagt
     4801 gtcaccgtgg tgttaaccag ccagacaata tccccggtca gtaacgccat ctgctcagca
     4861 gtcagcgcca cacccggttt caggttgtat tgcttaccaa atgcaatacc cgcatccatt
     4921 aatgccttaa actgctcttc atcactgccg tatccgtcca ggaaccgttg cccggtcagg
     4981 gcgacaacct gctcacgaat tatacgctgt tcgtaaaaac cgtctcccag gcgtttgagc
     5041 atgttatccc cgttctgaga aaacgccttc tgcatgtaat cactgcccag ccacatcttc
     5101 tggttcgtaa aacgcgggtc cgtttcaacc agatatggca cattcactgc cggatttgtt
     5161 ttaaacagac tgctgtccgg cagacgggta tccggcccgg caacatggat actgccctga
     5221 ggtgtgctga cttcaaactg ctgccccggc aacagaatga ccggttcacc ggctgacacc
     5281 accgggcgaa gcggtatccg gtcagtagca gctacagtcg catccacatt tccggcctgt
     5341 cctgtctgta catccgttcc ctgtggcttc aggggagaaa gcgtgacatg gctgccctga
     5401 acctgtccgt ggctggtgag ctctcccggc ttcagcgtga tggtctgaat tacagtgggc
     5461 ggagtgtact gagaggtttt ctccccctgc ttatcctttc cttttttcct gatgcggtaa
     5521 taatacgtgg atgttcctga gtccgtaaca tgccgctcgc cactgacact gacattttcc
     5581 acgcttcctg acggaatgct cagcaggccg cctgcaacaa cctgactctt atcattaagc
     5641 agtttgtctg ccacaaccgt catgccggca ccggacagaa tttttcccgg gtcggagtct
     5701 tttacccggg tttcttccac ggtacgggtg taatcgtact gcgtgaattt atctctggcg
     5761 ccttccggtg tatgcagttt tttcagtgag ttactggaat cgttatccac ataaacgccc
     5821 ggttcaccgg cgctccagcg tttatctgag ccgctgagct gatactcagt caccttttcc
     5881 gtggaaacca ccacgcgctc cgtggtaaaa tggtcattca cgttattgat ttgtccggcg
     5941 ctcagtgcca gggaaccggt ggcttcaaca gtggcactgt gattattcag tacaccggct
     6001 ttacctgtgg cgctgccatt cgcatccagc gtgccaccga tatgcatatc gcctgcactg
     6061 taaatcaggc tgtgcgtccg gttattcagg gtctgtaccc ctaagtccac acgctcacgc
     6121 ccggccagtg tcgccgccac gccattttct tccagattat taaatgtggc agcactgaca
     6181 ccaacagcat cgccgtagat tcgcccggta ccggaattcg tcagggtgtt cgcctgaaga
     6241 cgcgtatatt tcccgtcaat cagcccacgg ttcagcagtg tgtccgtcac gttaagccag
     6301 gtctgcccgg cactgatttc acctttttct gtgttttcaa ggcggatgct gttcagctca
     6361 agccggctgc ccgccagcag gctaccggta ttagtgacat cgccattaac ggaaagcgta
     6421 aggtttccgt tcgcaatagt ctgaccactg ttactgaacg ctgtgttact tttcagcgtc
     6481 atatcgcccg gcgaaagcag cttcccgtca ccaccgagct gaccggcatc cacagatact
     6541 gactgcccgg ccttaaccac gccgtcacgg ttcgaaaacg ccagctgacg gccactgaca
     6601 gacaatgtcg cgccggaaga taggacgccg tcattattac tgagcgtcct gtctgccata
     6661 accgtaagcg ccctgtctgc cagaatactg ccctcctggt tatcaaggtc ctgcgttgtg
     6721 atgtgtattg tctgcgcttc cagcccctgg ccggtattct gcgtgtggcg attgataaac
     6781 tgcagtgcat tcagcgtaat ggcggaacca ctgcgaatca gaccagagac gttgtccaca
     6841 acgccccgta cactgtcgag cagcatgtcg cccacagact gtatctgtcc gtggctgttc
     6901 tgcagacctc tcgtatgcag acggatttca ccttcactga ccagccggcc accctcacgg
     6961 ttatccagcg aggtggtgct caggtcagct gtcgttttcg cccccacggt cccgccccgg
     7021 ttatccattg ctccggaact gacagtcagt tcctgcaggc tgttcagcgt accgcgtctg
     7081 ttatccagcg tctggcctgc ggtattaatg ctgatacccc acccctgaat aagaccggac
     7141 tgattgttca gcgcctggct gttccagccg agcagaccgt ttgccaccac ctgaccggat
     7201 gtattgttaa ccacgcctgc agtcagggac agagcatctc ccgacaaaag cactccggct
     7261 gtgctgtcca gtgtgccgga attcagtgtc accgggccct gactgataac cccgccccgg
     7321 tcaccactgt cacggttgct cagtgtgtca gcacggatat tcagcgatgc accgctctgt
     7381 atcaggccgc cggcatcgtt gatgatgtct ttcgaggtaa gtgtcagtga ctgctgtgcc
     7441 acaagcctgc caccctgatt atcagagcgg gtgacatcca gtctgctgtt accgttgctg
     7501 accaccgtcc cctgctggtt ttccagcaca ttcaggtgcg cattcaggtc tccgccggcg
     7561 gcaagcgtcc cttcctgatt actgacatcg tcaccactca gcaacatatc cgtcccggcc
     7621 ttcagtgcgc ctttctggtt catcagcgta cctgacgata cctgtagtga cgcctggctc
     7681 cccagcgcgc cctgacggtt atcgagcgtt ccggtgttca gggtcacatc cttctgaccc
     7741 gccataaatc cctgctggtt attcagcata ccggcccgta ctgtcagggt accggcgctg
     7801 ctgataccac cccgtgtttt cgtgttccgg ttactgagta ccccgtcctg tacatccagc
     7861 gtcatgtcac tgccggactg aatcagacca tcatcagaga ccagttcgct gcctgacaca
     7921 gacagcgttt tctgtgaaag cagtctgccg gacgtattat ccagcgcacc atccagcgtc
     7981 acagacagct cgtcagacac cactgtaccc tggtggttat tcagattccc tgccgaaata
     8041 tccagcctgc cggcggactc cattcgtccc gccgtattgc tgacagcctg ctgtgttttt
     8101 atcctgcccg ttccggcagc agctgaaatc agtccgttgt cgttattcag cgcctgtgta
     8161 ttaatcagga tgtcacggct ggcaatcaga gttccctgca ggttatccac ggcgccggtt
     8221 gtcttcacct gcagactgcc tgactgcgcc gcctgcactt tccccctgcg attatcaagg
     8281 ctcagtgcat ccaggcgcac cgcgccattt gccgcaagca gcccttcccg gttatccacg
     8341 cttcccggca gactcagtga aaaatccccc gttcccgtct gaatcaggct gcctttatga
     8401 tttgacaggc tctgtgccga tatctgcagt gtgccggcat ttatcgtggc gccattattg
     8461 ctgaactgcc cggcagtccg ggcagcaaag gtaccggcgc tcagttgcgc attctgtgtg
     8521 gacagatttc cgctgcctgc atccagcccg gtatgttgtc cctgtatccg gctgttgctt
     8581 aaatccagcc cctgtccctt cagttgcatg tccccaccgg acagaacctg tccgtgtgcc
     8641 gccagttcgc cggtggtact gacagtcagg ttacccgctt ctgccagacg tccgtcatct
     8701 ttcaccccgg cggccagcag actccgctca ccgccggtca gacgcgttgt ctgaatccgg
     8761 gtgttgcgac gtgatgccac agagcctgta tgggtgaata caccatcact ctgtatctgc
     8821 gtatcctgcc ccgcataaac agaaccactg ttattcagtt cccgggttgt cgccagatgc
     8881 acatccgttt tcgcactgat actgccactg ttttcaatac gtccgtcagc cgtcagggta
     8941 accgccccgg cctgcgcacc aatatgaccg gcgttacgca cgcccacacc tgtttccgta
     9001 ccgacaaggc gaatttttcc ggcatacatt ccaccaaggc tggacacatc cagcgccacc
     9061 tgtggctgcg aagaggcatc agcggctttt ttctcagtct gcccgtgtgc aatatccaca
     9121 ttgttacgtc cggtggtcac tttcaggtcc tgcgcccaga cacccgcatt aatcttcaca
     9181 gaccgggcaa tgatatcggt ataatccgtg cgcgaggcat ccatcccttt gcccgtaacc
     9241 tgaatctcac caccttcaac acggaatccg gtcagactgc cgtttttcat ctgcggctgc
     9301 cccgtggtca gcgtggcccg gttcgcatta ataaagccgc acccgtcgca actgataccg
     9361 gccgggtttg caatcaccac ctgcgctttt ttcccggcaa cctcaacaaa accgttcaac
     9421 tggctgggat tgcgactgtt cacttcatta agaatgattc tggcctctcc cttagccagc
     9481 cacgggttcc cggccaccat gccacccagc tgagtctgca cattgttatg ggcattattc
     9541 agaatgacac cctggttacc gacgtcgaac tggctgtatg tgttatgtga caccccgccg
     9601 gcagaaggcg tctgaatatt cacctgcgga atgccgctgg ccgtctgcat aatcgtgggc
     9661 tgctgatgcc ccggtgcgcc atggtctgcg acaatgcctg cagcgctgac agaatgcacc
     9721 atccccgagg ccagccacag ggagaatgca agcggcgtga cacggcagat acgttgcctg
     9781 tgtggatatc ctgttcggga ggagggtgat gcaccggcgc gaccggaacg ggcaatatct
     9841 gccaccacca tcagcattcc ccgggcttta ttaaatatga tgcggtaaca atttctgttc
     9901 attctgataa acctgactta tctctccggc acatcaacgg caagcgggtg ctgcacacgc
     9961 cactggcgcg cttcttcacg actgacctgc acacctctga aattcatcac ccgtaacccg
    10021 gtctgctgcc cacggtggta cagcgaacgc cagcacagac ggggaaaaat atcctgccgg
    10081 acaagacgac tcatatccgg ggtatgcccg aaaggggcaa cccagtcaca gacccacatc
    10141 cggtcgccgc tgttccagtc cgactgaggc atgagaattg ccggctgcgt aagataacgc
    10201 gcctctgcat ccaggctgag ccaggcctgg ctcatgaaga aaacaggtcg cccctgctct
    10261 gttgccacca catactgtcg gtgcttaatc accggcagca gcaggtccgg cagggtatgc
    10321 agtggcgcat cacgatgcag gggagagtgc atccacaacc acacgaccgc tcccagcact
    10381 tccgcttcat tgaccggctc accggaatac agggggctca gaacgtcata tttcccgtta
    10441 cgcatgaacg tttctcagaa atcccggaac agattaaaac cggctgtcac atcactcgtc
    10501 ataaaacccg ccggttttga aaacggacga ccgatgaaaa cgtcataatc tgtactgaac
    10561 agttgccccc tcagccccac aacaccaccg gccagatgac gtcccacaag gtattcactg
    10621 ccggctcccc ccacctcgcc gtaatccatt cccagatata actcctgctc cggcagtggc
    10681 gtacgccagg ccaggtcgtt gcggatatac cagccgttac tggcattcag cgttcgctcc
    10741 ccgtcaaaac cacgaaccgt ccagcggtta ccgatggcaa actgttcctg tggggtcagc
    10801 ggggtattgc tcagctggcg ctgataccgg aaggaataac tgaagggctg ctctgccagt
    10861 gtaaagggca gattaaagga tgcattcatc tgcaggatct tactgagcgc cgttgcctca
    10921 ccgaagattt cctccggtgc cggctgtgca ccaaaccagc gggttccccg ttgccagctg
    10981 acaccagcat ccagtgtggc ctgagaaata taatgacggt gctgcagccc aagtcgccag
    11041 gccgctgtct gccggcgctg cacctggatt tccgtatcat taatgtagtt acgcgacctg
    11101 cgggcctgaa catcgtaagt cagcaccgtt ttctgcgtcc cgttacggtg cagtacccgg
    11161 ctcagctgca tgcccagatt ctgactctcg ccactgtact ggtaatcctc aaccgcacca
    11221 gctaccgtct gatggtaatc gtaatcactg gcggtgacgg aaaactgcca gtaaccgaaa
    11281 ggcacagagt aatgggccgt gtagtttttg ctgcccttac ccccgtaatg tggaaaatta
    11341 tgggtggcag aaacatacag caggtcgctc agggaaaaag gattatccag agaaaatgtt
    11401 ataccaccga gataacgccc tgtgctgcgg gtacctgaat catcaagcga aaaccccagt
    11461 cgccagtatt tgtcctgttt gcgggtaata agaatgtcac tttcccctgg ctgctcaccg
    11521 ggcacaattt ccatatcagc ctgcacaccc ggcagtcgtt gcatattctc cagcccctgc
    11581 tcaatgtccc gtaaatccag cagagcgcct tcgtgtgccg ggaatgagga atacaactga
    11641 atatagtcat cactgtccgg tgtcagacgc acatgtcgca cgacaccggg aataataagc
    11701 agccggagaa taccgctttt taaatcctgc gaaggcgcca gaacccgtgt ggtgatataa
    11761 ccatgatcga ccagacggtt ctgcagtgta ctcatcagca ggttgatccc ttttgccccc
    11821 agacaatgtc cgaccgcccc gttggcgatt ttttgtaaag gcaaccagtg tggtaacgca
    11881 tcagccccct ccagttccgt ctgtttaatc tgaaaacagg gcgtttcaac aggaaaatta
    11941 atcttacggg taaaagagcc aggtgcagaa aggagaacat caggggctga aggcgtcagt
    12001 tgttgctcaa gggcttcctg acgctgctgc tgaatgataa atggggcatt tccgttatgc
    12061 cgggtatcag cagcctgaag atatgaaggc aataataaag caggtatcag tgtcagtgat
    12121 aaaatgaacg gacagcgccg gaatacgcgt tgctcttcag ataccacgcc tctgcgacat
    12181 aattttccag ccaaacgaaa aaccttttat gcatgctgtg tcaacaatca cctgtcagcc
    12241 tcctgacgat gtctgccgcc attattggca ataacacact accgcccttc cggaattccc
    12301 cgtatcaacc ggtaccagaa tgctgagtaa ccagtcagcc aaatcaggct gctttaattt
    12361 tgctcattat agaataatga tctgaaaaat atcaatataa caggtgtatc gatcgtaatt
    12421 gcagatgctt gcagatcttt atttcagtat atattgttaa gctggaatat ttagaaatat
    12481 atatccatga ctaatgtgga aatacagaaa attattccag tgaatgcttt ttatacggtc
    12541 tcctggcaca gaaaatggtg atttttgatt tgaatcattg aaatcagttc tgctaataag
    12601 aagacatgct gaaaagccga ttttcagggg gtgattgaag ggattgtgag tttttccttc
    12661 ggcttttaac gtaacttcct gttttcctga tattccacgg caagattaat ttttagcgga
    12721 gcagaatgaa taaaatcttc atctggtgtt tttgttgcgc gtaaatgcag gtgtaactgt
    12781 ggatgttatc cactgaagag ctgctgatga ggatgaagac atgctgacta aaaaaatgtt
    12841 acttgtttgt gcttcgtgtg ttgcgctgag tgcctgttct gcacaggaaa ttgcagagct
    12901 gaacaggcag ataagtgacg gtgcatttgc tgtgacaaaa gtactgaacg taggtgtaaa
    12961 ccaggcagat gatgatct
//
LOCUS       DAZFFI010000125        12904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFFI010000125 DAZFFI010000000
VERSION     DAZFFI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.125"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..341
                     /locus_tag="ACHJ12_004124"
     CDS             24..341
                     /locus_tag="ACHJ12_004124"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO4991833.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            410..739
                     /gene="yjdP"
                     /locus_tag="ACHJ12_004125"
     CDS             410..739
                     /gene="yjdP"
                     /locus_tag="ACHJ12_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4991834.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(886..1644)
                     /gene="phnP"
                     /locus_tag="ACHJ12_004126"
     CDS             complement(886..1644)
                     /gene="phnP"
                     /locus_tag="ACHJ12_004126"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4991835.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1646..2080)
                     /gene="phnO"
                     /locus_tag="ACHJ12_004127"
     CDS             complement(1646..2080)
                     /gene="phnO"
                     /locus_tag="ACHJ12_004127"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO4991836.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2067..2624)
                     /gene="phnN"
                     /locus_tag="ACHJ12_004128"
     CDS             complement(2067..2624)
                     /gene="phnN"
                     /locus_tag="ACHJ12_004128"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4991837.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2624..3760)
                     /gene="phnM"
                     /locus_tag="ACHJ12_004129"
     CDS             complement(2624..3760)
                     /gene="phnM"
                     /locus_tag="ACHJ12_004129"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4991838.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(3757..4437)
                     /gene="phnL"
                     /locus_tag="ACHJ12_004130"
     CDS             complement(3757..4437)
                     /gene="phnL"
                     /locus_tag="ACHJ12_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4991839.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            complement(4548..5306)
                     /gene="phnK"
                     /locus_tag="ACHJ12_004131"
     CDS             complement(4548..5306)
                     /gene="phnK"
                     /locus_tag="ACHJ12_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4991840.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5303..6148)
                     /gene="phnJ"
                     /locus_tag="ACHJ12_004132"
     CDS             complement(5303..6148)
                     /gene="phnJ"
                     /locus_tag="ACHJ12_004132"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4991841.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6141..7205)
                     /gene="phnI"
                     /locus_tag="ACHJ12_004133"
     CDS             complement(6141..7205)
                     /gene="phnI"
                     /locus_tag="ACHJ12_004133"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4991842.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGERPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7205..7789)
                     /gene="phnH"
                     /locus_tag="ACHJ12_004134"
     CDS             complement(7205..7789)
                     /gene="phnH"
                     /locus_tag="ACHJ12_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4991843.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7786..8238)
                     /gene="phnG"
                     /locus_tag="ACHJ12_004135"
     CDS             complement(7786..8238)
                     /gene="phnG"
                     /locus_tag="ACHJ12_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4991844.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8239..8964)
                     /gene="phnF"
                     /locus_tag="ACHJ12_004136"
     CDS             complement(8239..8964)
                     /gene="phnF"
                     /locus_tag="ACHJ12_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4991845.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8985..9764)
                     /gene="phnE"
                     /locus_tag="ACHJ12_004137"
     CDS             complement(8985..9764)
                     /gene="phnE"
                     /locus_tag="ACHJ12_004137"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4991846.1"
                     /translation="MQTITIAPSKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVQPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9832..10848)
                     /gene="phnD"
                     /locus_tag="ACHJ12_004138"
     CDS             complement(9832..10848)
                     /gene="phnD"
                     /locus_tag="ACHJ12_004138"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4991847.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10873..11661)
                     /gene="phnC"
                     /locus_tag="ACHJ12_004139"
     CDS             complement(10873..11661)
                     /gene="phnC"
                     /locus_tag="ACHJ12_004139"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4991848.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11794..12237)
                     /gene="yjdN"
                     /locus_tag="ACHJ12_004140"
     CDS             complement(11794..12237)
                     /gene="yjdN"
                     /locus_tag="ACHJ12_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4991849.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12397..12732)
                     /gene="yjdM"
                     /locus_tag="ACHJ12_004141"
     CDS             complement(12397..12732)
                     /gene="yjdM"
                     /locus_tag="ACHJ12_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4991850.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2552 a         3632 c         3903 g         2817 t
ORIGIN      
        1 caaatactga gcacaattta tcaatgcaac cggtgtttct ttttaaaaaa cgtaccggag
       61 caatgggcgg tcatgtcgct ggcaccgcag gattctgcgt tgctgtgcca gccttttatt
      121 catggtcaat gagtgaggtg attgataaag acaccaggtc gtcagagcgc actgattatc
      181 cgcattatgc cagtgaagtt gcactggctt ttggttcacg agtggttggc ctcgaactgg
      241 caaaaatgat tgtggatgcg tggctgggcg cacagtacga aggcggtcgt catcaacaac
      301 gcgtggaggc gattacggca atagagcagc ggagaaattg agattcatcc actacttgca
      361 tggatgagta atgattaatg tggatagagt ttctttttga ggttggctaa tgaaacgctt
      421 tccgctattt cttttattca ccctgctcac gctgtccacc gttccggccc aggctgatat
      481 tatcgacgat actattggta atattcagca ggcgattaat gacgcttata accctgaccg
      541 tgggcgagac tatgaagatt cgcgcgacga cggctggcag cgtgaagtga gcgacgatcg
      601 gcgcagacaa tatgacgacc gccgccgcca gtttgaagat cgccgcaggc agctggacga
      661 tcgccagcgc cagcttgatc aggagcgtcg gcaactggag gatgaagagc gaagaatgga
      721 agatgagtat gggcgatgag gttgggtatg gagcaggcat cgcccctcac cccaatgggg
      781 agagggagaa aacgagcgca atattcaata tcgagcacaa tcggtcccct cgcccctctg
      841 gggagagggt tagggtgagg gggccagcgc gcacattccc tctcatcacg ccaccccaat
      901 ctccatccca tcaaacccca cctcaaaccc tgacggtagt gcgttttcca tcagccacgc
      961 atcaaactgg tggctgatat gggtcagaat cacctgtggc gagcggataa cctgattcag
     1021 cgcaagcacg gtatttaaat cacagtgatt acgcggtgca tccgcgcgcg gcgggtgact
     1081 gcaatcaatc accatcacct gcggatgatt attgagtaaa aatttcagcg ttttttccgg
     1141 caggccagcg gtgtcagaca gccacgccac ccggctatgt gccgtttcca gcagataacc
     1201 gaaggtcagt tttgagtggt taagcggcag cggcgtgacc tgcaacccct gcaaatcaaa
     1261 caccacaaac ggttccactg tgtggctgaa atcaagcagg cccggatgtt taaacagatc
     1321 gtcgcagccc tgttcatccg gcgggccgta gaccgggatc acatcgccca cgccccagcg
     1381 tagcggaaat agcccctgga cgtgatccat atggtagtgc gtcagcaaaa actgctggaa
     1441 cgatccaggc gaccagcgat cggtgagatc gtgccgcccg gcgtcgatca gggtgatcgc
     1501 gtcgttaaac ttcactacgc cgctgcatgg ctggcggcga tactgcggcg agcgccgcgc
     1561 tctggcgcag gccgcgcact cgcagcccca tgccggaacg ccctgtgcgc cgccagtgcc
     1621 ggtgagcgtg agggtcaggc tcatgttaca gcgccttggt gaagcggaag tggctctgct
     1681 cgtagccttc acgcaggtaa aaacggtgtg cgttgtggcg cttcacgttg gtggaaagct
     1741 cggtcatttc ggccccggcc tggcgggctt cttcttctgc ccatgccagt aatttactgc
     1801 cgacgttcag accgcgcgcc tgcggcatca ccaccagttc ttgaatttcg ccgatccagt
     1861 tgacatgatg cagatgaaac tgcaaatgca ggccgatcat gccgacaact tcgccatcca
     1921 gcagcgccag atggtagcgc atgtttgggt cgcgcaggtt ggcgttaaag cccacgcgaa
     1981 acgcatggtg gtcaaactcc gcctgtttta gctcacaaat cagcgcgtaa accgcgtcgg
     2041 tatcgtactg cgtggccggg cgaagctcac aagcaggcat ggtgtttctc cttctgatgg
     2101 atcagcgtca gcagcgtgtc gaccgactgg cgcaggctgc cgtcattgtt gagcgtatgg
     2161 caatcctgtg gagtatagcg ggcggcgcgc gccaggcggg cgttaatttc actggcgttt
     2221 tcacggccac ggttttccag gcgctggcgc aggatctccg gcgaaacctg taaacagacg
     2281 ggcagcagcg ccgattgata gcgcgcccgt gcctgcggca aatgcgcgcg tgaaccgttg
     2341 acgactacgt cgaatccagc gtgcagccag aggtcaatct cgatgccgac gccataatac
     2401 agaccgttag cgtgccagct taaggccagc aggttttgcc ccgcgcgggt aaaaaactcc
     2461 cgctcactca gggcgatatg gttttcactt ccggcgctgg cgtcgcgcgt gatgtagcga
     2521 tgcgccacca gtaactgcgt ttgttcccgc aggcggagtt ccgccagcag gctgtctttc
     2581 ccggagccgg acggccccat taaccaaatc agttttccca tcatcagaac acccttttac
     2641 cctgacgcca gacgtggtcg atatgaatgt gattgccctg gcgatgcgcc agcaccaggt
     2701 cagcgcgttt accctcgcca atcaccccgc gatcctgaag attcagcgcc tgcgccggat
     2761 ttttggtcac cagcctcacc gcctgcggta gcgtaaagcg gttgctctcg tcatcggcga
     2821 cgcggaacgc cgcatccagc aggctggcgg ggtagtagtc ggaagagagg atatccagca
     2881 gaccaagctg cgccagttca ctggccgcca cattgccgga gtgcgagccg ccacgcacga
     2941 tatttggcgc acccatcagc acgttcatgc catgcttacg cgaggcttcc gccgcttcga
     3001 acgtggtggg aaattcggcg atcacgctgc caagttggtg ggattcggca acgtgggcgt
     3061 gtgtggcgtc atcgtggctg gcgagcgcaa tttgtcgcgc gcggcacagg gcggcgatgg
     3121 attcgcgatt cggctgcgac cagcgtgcgg cgagcgccaa ttgctcttct tcgtactgct
     3181 gcatctgcgc atcggtgagg gagtatttgc cctgataata ttcgcgatac ttctcgcggt
     3241 tagcgaactg gcgctggccc ggtgagtggt ccatcagcga caccagcgtc accggttcgc
     3301 gctgaaccag tttttcaaac agcggcagtg tggtgtgatg cggcagttcg cagcgcaggt
     3361 gcaggcggtg ctcggcgcgg ttgacgccgc gtttctgcgt ctcttcgatg gtgttgatca
     3421 tcttctccag attctccagc cgatcgccgc cgtcgcgcac gtcgccaatc gccaccgcgt
     3481 ccagcacggt ggtgatgccg cttgccacca tcagcgcgtc gtggctgctc atcgccgaat
     3541 gggcgggcca gtcgactttc gggcgcgggg tgaagaattt atcgaggttg tcggtatgca
     3601 gctcaatcag gccgggtagt agccagccac cttcgccgtc catcgcttct ggcaggcggc
     3661 tctggctttc ggcaaaggcg cggatttcgc catcctgcac ctccagcgaa ccgtgtacca
     3721 cttcgttttc cagcaccagt ttgacgttat tgataatcat gcagaggctc ccattgggtg
     3781 caagcggtcg gcgacgtcat tacgtacggc ttcgtcatgg aagatgccga cgatggccgc
     3841 gccacgggtt ttggcttcgc gaatcagctc gactacggcg gcgctgtttt tggcgtccag
     3901 cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg acgataaagc cgcgggcgat
     3961 gttgacgcgc tgctgttcgc caccggaaaa ggttgatggc gccaggtgcc acaggcgttc
     4021 cggcacgttc aggcgggtga gcagacgcgc ggctttagcg gcgcaggctt cacgcggaac
     4081 gccggtatcg agcagcggct gcatcaccac ttccagtgct gaaatacgcg ggatgacgcg
     4141 cagaaactgg ctcacccagc cgacggtggt tttgcggatc tccaccactt tgcgggctgg
     4201 cgcggtgacc aggtctaccc actcgtcacc gtgtttgatc tggatttgac cttcatcggg
     4261 cagatagttg gcgtacagcg agcgcagcag ggttgatttg ccgctgccgg agtgaccgtg
     4321 gagcaccacg cattcgcccg cgttgacggt gagcgaggcg cggttgagga cgggcaggcg
     4381 cacgccgttt tgctggtgca ggatgaaggt tttactgacg ttttgtacgt taatcatttt
     4441 tcgcctcgtg gttcgggtgt cggatgcgac gctgacgcgt cttatccgac ctacggggag
     4501 cgcatttgta ggccggataa ggcgtttacg ccgcatccgg caccggctca attctgcaaa
     4561 accgatgaca ccagcagctg tgtatacgga tgatgcgggt cgtcgagcac gcggtcggtt
     4621 aacccactct ccaccacttg cccctgcttc atcaccagca aacggtccgc cagcaggcgg
     4681 gcaacgccta aatcatgggt gacaatcacc accgccaggt tcagctccac caccaggccg
     4741 cgcagcaggt cgagcaggcg ggcctgcacc gacacatcca gcccgccggt cggttcatcc
     4801 ataaacacca gcttcggatg cgttaccagg ttgcgggcaa tctgcaaacg ttgctgcatg
     4861 ccgccggaaa aggtggtcgg caggtcgtcg atccggttgg cgggaatttc cacctcttcc
     4921 agccacttct gcgcggtggc gcgaatgtcg ccgtaatgac gcgccccggt cgccatcagc
     4981 cgctcgccga tattgccgcc tgccgagacc tgacggcgca ggccgtcgag tggatgctga
     5041 tgcactacgc cccattcggt gcgcagcagg cgacggcggt cggcctcgct cattgcatac
     5101 agcgaacggt tctcgtagtg aatctccccc tgctgcggcg tcagacgcgc ggagatcgac
     5161 ttcagcagcg tggtcttccc ggagccggat tctccgacaa tgcccagcac ttctcccggc
     5221 cataaatcaa aagagacatc gctaaagcct ttgcccggcg cgtaaaggtg ggtcaggtta
     5281 ttgaccgaaa gtaacggttt attcattggc ttttcgcctc gctctgttgg cggcaataat
     5341 cggtatcgga gcagacaaac atgcggtttc cggcgtcatc cagcaccact tcatcaagat
     5401 agctgtgggt cgatccgcag atggcgcatg gctcatccca ctgctgaacg gtgaacgggt
     5461 gatcgtcgaa atcgagactt tccacgcggg taaacggcgg caccgcatag atgcgcttct
     5521 cgcgccccgc gccgaacagt tgcagggcgg gcatcatgtc cattttcggg ttatcgaatt
     5581 tcgggatcgg cgacgggtcc attacgtagc gcccgttaac cttcaccgga taggcgtagg
     5641 tagtggcgat atgaccgaag cgggcaatat cttcatacag tttcacctgc atcacgccgt
     5701 actcctccag cgcgtgcatg gtgcgggttt ccgtttcgcg cggctcgata aagcgcagcg
     5761 gctcggggat tggcacctgg aaaataacta tctgatcttc ggtgagcggc gtttcgggga
     5821 tgcgatggcg cgtctggata agcgtcgcat cgtccgtccg ttcagtggtg tttaccccgg
     5881 tcacgcgctt aaagaagttg cggatcgaca cggcgttggt ggtgtcgtct gcgccctggt
     5941 caatcacctt cagcacgtcg ctttcgccaa tcacgctggc ggtgagctgt atgccgccgg
     6001 ttccccagcc gtatggcatc ggcatctcgc gcccgccaaa cggcacctga tagccgggga
     6061 tcgccaccgc ttttaagatg gcgcggcgga tcatgcgttt ggtctgctcg tcgaggtacg
     6121 caaagttgta gccgctcaga ttagccatgg ttctgctcct gttgcagacg tttgagtagc
     6181 tccagttcgg cctggaaatc gacgtagtgc gggagtttca ggtgcgagac aaagcctgcg
     6241 gcttcgacat tgtcggcatg cgccagcacg aactcttcat cctgcgccgg gcctgtcgcg
     6301 tgctcgccgt actctggagc ctgcaacgca cgatcgacca gcgccattgc catcgctttg
     6361 cgctcgctca tgccgaacac cagcccgtag ccgcgcgtga agtgcggccg ctcacccggc
     6421 gggtcgataa aaccgttgac catttcacac tcggtcatca gcagttcgcc gacgttcacc
     6481 gcaaatccca gctcttccgg cacaatcgat acgtcgatat agccgctgcg gatctcgcct
     6541 gcgaacgggt ggttgcgacc gtagccgcgt tgggtggagt aggccagcgc cagcaaatag
     6601 ccttcgtcgc cgcgcatcaa ctgctgtaaa cgggaggagc gtgagcaggg gtaaaccggc
     6661 ggcgtgcggg tgatgtcatc cggctgtgcg ccgctatcct cttcaaactt cgccagcccc
     6721 tgacgcgcca gcaggctgaa aacgtgcggc gacggctgtt gttcgctgtg ggcggtggtc
     6781 agcgtcggcg cttcgccgtt tgccagcagg gtgaaatcga gcaggcgatg ggtgtagtcg
     6841 taggttgggc caagcaactg gccgccggga atatctttat aaaccgctga aatacgccgt
     6901 tcgagacgca tctccgtggt gtcgagcggc tcgcttaccg cgagcttcgc cagcgtggtg
     6961 cggtaggcgc gcagcaggaa aattgcttcg acgttatcgc cgctggcctg tttcagcgcc
     7021 agcgccgcca gttcgcggtc ggcaatgccg ccttcggtca tcacgcgatc tactgcgagg
     7081 ttaagctgct gttcaatctg ggcgacgctc agttcaggca aatcggtatc gccccggcgt
     7141 cggctctctt gcagggcgtg ggcggcatcg atcgcctttt cgcccccttt cacggcaacg
     7201 tacatcagca cacctccaca tgcgtggttc gcggaatagc cagcaggcgc tcgccgcagg
     7261 tcaggatcag gtcgatgccg agcgggaacg ggtgcgggcg ctcggtgagt tcgtacagaa
     7321 tgcactccgg cagctgcggg gcgatcattc gttcttcggc aatgcccgcg ccggtgagac
     7381 gtaacatgcg tccgccgctc aggctggcga cctgtaaaat cagcgttgca cccgcttccg
     7441 gcgcaacggc ggtgccggtg gacagggcgt taagctgttc gctggaaatc gcctcatccg
     7501 ttaccgcgaa ggtcgcctgt tccggctggc tgaccagcgg cgcgttggta tgaaaacgca
     7561 ggctctggct gacgatgtcg ttactcaatg gcgcggaaag ccacaccggc gtgtcgttat
     7621 cggccagcgt cagcagcacg ctggtggtgg cgatattcag cggctgccag ccgcgtttga
     7681 gctgatgcag ggcgacaatc acgcccggct cgctcatggc ctttaacagg cgacgaaaac
     7741 tgtgctgggc atcctgcaca ggaagaataa aagcggtttc cagggtcatg cgttatctcc
     7801 gcgaaccatc gtaaagaagt cgacccggct ggcgttcact tcggcctggc gtgcggcaat
     7861 gcgtgccata cggtcagcgt ccagcggggc aataagagtt tctgataagt tttgaaagtg
     7921 gcgagattgc tgcatcagcg cgtcaatcag cgcgcagcgt tcggcgtgct gtttgtcgcg
     7981 ccccagcacc cagctgtagc cgagcgtgcc gtcagtcagg cgtacggcgg cgcgggtcag
     8041 cgtggcgtcg ccggcaaaaa agcgttcgcc ggtaccgccc attcgcgcct gaatctgcac
     8101 caggccagtt tcggcagtgc ggatcacttc atagtcggcg gtgatgttca gcgcgttcag
     8161 gcgggctgcc agttcagccg gttggctgtg cgccagtacg gacatccagt gctggcgggt
     8221 cgcggtatct gcgtgcattc agtgctccat agtgaattca atcatgtcgg cgcgcgtcag
     8281 gctgacggag tactccgccg ggctgctttc accgtcacgg tggttaaggg tgcgcacgca
     8341 cagcagcggc gacatattcg ggatttcaag gcgctggcac tctttggcct gggcgcggcg
     8401 ggcgctgatc cgcgtctggc tgcggcgcag cgcaattccg gtttgctcgc gcagaaaatc
     8461 gtgcagcgaa ccgctgtcga aacgttgcag tgtcggccag agggtgaggt ccgcaaagta
     8521 gtggtcgatt aaacagagcg cgatgccgtt gacccggcgc agggtgcgca ggtggatgac
     8581 gttctccccc tcggttatcc ccagcgcgtc agcgacgtga ccagatgccg ggcgcagcac
     8641 cgacagcagt ttttcgctgg tgggatggct gccctgatcc agcaggttct ggctaaaacg
     8701 cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc accagcacgc cgacgccctg
     8761 acggcgctgt acccagcctt tttccaccag ttggtcgatg gcgcggcgca gggtgtggcg
     8821 atttacctca aagcgcgccg ccagctgctg ctcggcggga agatagtcgc cgcagcgata
     8881 gtgttgacga agctcctgct caagttttgc ggctatctcc tgatagcgtg ttgggtagct
     8941 ggtcggatgt gtagacaagt gcatagatat caatgcctcg cttatcagat aaagtgcttg
     9001 cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga cgatgataag caccatcagg
     9061 gcgcaggttt gctggaactg gaaaccgcga atcgcttccc acagggtgac gccgatcccg
     9121 cctgcgccga ccatgccgac gacggtcgcc gagcggacgt tggattcgaa gcgatagagg
     9181 gagtaggaga tcagcagcgg catcacctgt ggctgcacgc cgtagaggat ctcttcgagc
     9241 ttgttggcac cggtggcgcg aatgccttcc accgggccgg gttcaatcgc ttccaccgcc
     9301 tcggaaagca gcttggagag cacgccggtg gtgtggataa atagcgccag cacgccagcg
     9361 aacgggccga gaccgacggc gaccacgaac agcatggcga agaccatttc gttaatggcg
     9421 cggcaggcat ccatcaggcg gcgaacgggc tggtaaaccc accacggcac caggttttcg
     9481 gcgctcatca gaccgaaggg gatggagaga accactgcca gcgcggtgcc ccagacggcg
     9541 atttgcagcg tgacggccat ttcggtgagg taatcctgcc actggctgaa atcgggcggg
     9601 aagaagtcgg cagcgaaggt tgccatgttg ccgccgtctt taatcagcgt aagcggggcc
     9661 atttccgcgc cctgccacga gacgaccagc acggcgagaa caacggccca gctcagaagc
     9721 gagaaccagc tacgcttgga tggggcgatg gtgatggttt gcatgtttgg ctccgtttaa
     9781 tgttttctcc ctaaccctct ccctcaaggg agaggggacc gatcgagcac attactgcac
     9841 cgctttactc accgaactca tcgcgcttaa cgcgttgttc aggcggtcca ggtcatccag
     9901 ctgcgcctga atctcggtgg ttttcgccag cttgtcctgc tcattcagtc ctttattgct
     9961 ttttacgccc tgcatctctt taaacagtgc gagctggcga atcggcacca gttgcaggtc
    10021 gctggaggcg cggaacgggg cccagcccag gcgttccagc accgctttct cttccggcgt
    10081 ttttccgtag ttcataaaga agtcgtagat cttgtctttg gtggtttcgg agagattctt
    10141 gcgccagacg atcggatcgc ctgggatcag cggcgacttc cagatcacct tcagttcttt
    10201 cagcttctct ggcgcggagg ttttcagctt gtcgaggttt tcggtgttgt tggtggcaac
    10261 atccacctgc ttgttggcga cggccagcgc gttggtttca tgcccagcgt tgacggtgcg
    10321 cttaaagtcg ctggcggaga tattgttttt ggcaaagacg tagtaaccgg ggacgaggaa
    10381 gccagaggtg gagttaggat cgccattgcc gaaggtgaga tctttccgct tcgccagcag
    10441 atcgttcagg ttgttgatcg gactatcttt gttgacgatc aacacgctcc agtaacctgg
    10501 cgatccatcc gccgcgaccg tctgggcgaa gacctggccg ttggcgcgat ccaccgcttc
    10561 cattgccgac aggttgccgt accaggcgat atccacttta ttgaagcgca ttccctggat
    10621 aatgcctgcg tagtctgggg caaagaaggc gttcaccttc acgcccagct tcttctccat
    10681 atcctgtaag aatggcgtcc attgcggttt caggttttgc tgtgattccg ttgaaataat
    10741 gccgaaattc agcgcctttt cctgctcttc ggcgtgtgcc gggcttaaca gggtgctgag
    10801 gctgaacatg ctggtgaagg ccagcgaggc aattatctta gcgttcattc gttttcctca
    10861 atgatgggga tgtcaggcag ctttcgcgtt ctcttcgatg cgattaatgc tgcggtagag
    10921 atggtcaaaa cgttcgttat caaactgttg gctgctgccg tcgtagaaaa cgtgcccctg
    10981 gcgcagggcg acgatgcgtt cgcagtagcg cagggcgtaa tccacctgat gcagcgtgac
    11041 gaccacggtg atgccgtcgt tctgattgat gtcgcgcagg gtgtccatca cgatgcgggc
    11101 ggattccggg tccagcgagg cgatgggttc atcggccaga atcaccttcg cctgctgcat
    11161 cagcgcgcgg gcaatcgcca cacgctgctg ctgtccgccg gagagggtgg aaacgcgctg
    11221 atgggcaaaa tgcaccatgc caacgcgggt cagcgcctgt aacgcgcgct gtttctgctc
    11281 gccggtgaac cagctaaaac aggtgcgcca gaacggcgtg ctgccgagcg cgccaatcag
    11341 cacgttctcc agtacgctca ggcggttcac caggttgaat tgttggaaga tgtagccggt
    11401 gttggcgcgg cttttgcgga tatcgcgcgc cagacggcct tcgcgctgga ctgtgcggcc
    11461 cagcagctcg atatggctgc cggcggattt atcgccggta atcaaaccgc ttaagtgacg
    11521 taaaagggtg gatttgccgg aacccgacgg cccaagcaga gccaccattt caccgtgatg
    11581 aatgttcaga tcaaccgcat gcagcgcctg atgctgattg aaggttttgg cgagcttctc
    11641 gacacggata atcgtttgca tgatgcagcc tccctaaaaa agtggctcca tcgtggcgga
    11701 ttattgtgac gatttggtta attaaaagtg actaacagat gaagagttaa cgggaattcg
    11761 atgacagtgc gggagggcgg gcctcccggc gggttattgc gttggttgtt gtttgacgac
    11821 attaatcatc cacggtacgc caaatttatc ggtgactttg ccaaagccat gcgcccagaa
    11881 agtttcctgc caggccattt cgatttttcc gttagcggca agattgtcaa accagcgttt
    11941 tccttcttcg acctgttgcg aatcgagcac cagcgtaaag ccggagtagc tggcttttcc
    12001 tgacggtata gcatcgctca tcatgatgtc gcttccggca atgcgcacgt tggcgtgggc
    12061 gatggcggta tcgggaaatt gcattccgga agggcagttc tcggcgctgt cctgcgctga
    12121 ttttggcatt tcgccgaagc tgattttata gagcagttca gcgcccaacg tacgttgata
    12181 ataagcaatt gcgtcggcac agttaccggc aaaagagagg tagggactta acggcatgat
    12241 ggtgacctca gttaagagaa gccagttaag tgtagttcga aatttataca gatgagagag
    12301 aagcctgata agacgcggtc agcgtcgcat caggcagtcg gcatcgttgc cggatgcggc
    12361 gcgagcgcct tatccggctt accgatcaca atacaatcag ttctttttca caaactcaga
    12421 tttcagtttc atcggaccaa aaccgtcgat tttgcaatcg atgttatggt cgccttcaac
    12481 caggcggatg tttttcactt tggtgccaat tttcagcatc gaagagctac ctttcacctt
    12541 cagatcttta atgatggtaa cgctgtcgcc gtcagccagc agattgccgt tagcatcttt
    12601 aacgatcagc tcgtcgcttt cctgtgcagg ttctgcgtcg ttccattcgt aggcacattc
    12661 cgggcagatg tacatgccgt tatcttcgta agtgtattcg gagttgcatt ttgggcagtg
    12721 tggtaatgac atgtggattt cctcaaaagt cagcagggca aaagcgccaa aaaacggcag
    12781 attgccgaaa aaggccgcaa ttatacacaa aatccctgag tttgtcgggg gtactgctga
    12841 ttttagacga cgatcgtttt catttgaaag gaaacgcaaa aagaaaataa tctttttgcc
    12901 tcta
//
LOCUS       DAZFFI010000126        12701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFFI010000126 DAZFFI010000000
VERSION     DAZFFI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.126"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(273..521)
                     /locus_tag="ACHJ12_004142"
     CDS             complement(273..521)
                     /locus_tag="ACHJ12_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4991851.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
     gene            740..2326
                     /gene="prfC"
                     /locus_tag="ACHJ12_004143"
     CDS             740..2326
                     /gene="prfC"
                     /locus_tag="ACHJ12_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4991852.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFIFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            2719..3324
                     /gene="osmY"
                     /locus_tag="ACHJ12_004144"
     CDS             2719..3324
                     /gene="osmY"
                     /locus_tag="ACHJ12_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4991853.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            3451..3612
                     /locus_tag="ACHJ12_004145"
     CDS             3451..3612
                     /locus_tag="ACHJ12_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4991854.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            3734..4795
                     /locus_tag="ACHJ12_004146"
     CDS             3734..4795
                     /locus_tag="ACHJ12_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4991855.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTLTRHLVPVVTPESIVIPPAPVANDTLVA
                     EVSDAPQANDPTFNNEDLA"
     gene            4792..5574
                     /locus_tag="ACHJ12_004147"
     CDS             4792..5574
                     /locus_tag="ACHJ12_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4991856.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(5687..6550)
                     /locus_tag="ACHJ12_004148"
     CDS             complement(5687..6550)
                     /locus_tag="ACHJ12_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4991857.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(6522..8072)
                     /locus_tag="ACHJ12_004149"
     CDS             complement(6522..8072)
                     /locus_tag="ACHJ12_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO4991858.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVIILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            8330..9109
                     /gene="deoC"
                     /locus_tag="ACHJ12_004150"
     CDS             8330..9109
                     /gene="deoC"
                     /locus_tag="ACHJ12_004150"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4991859.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            9236..10558
                     /gene="deoA"
                     /locus_tag="ACHJ12_004151"
     CDS             9236..10558
                     /gene="deoA"
                     /locus_tag="ACHJ12_004151"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4991860.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPVV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            10610..11833
                     /gene="deoB"
                     /locus_tag="ACHJ12_004152"
     CDS             10610..11833
                     /gene="deoB"
                     /locus_tag="ACHJ12_004152"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4991861.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            11913..12632
                     /gene="deoD"
                     /locus_tag="ACHJ12_004153"
     CDS             11913..12632
                     /gene="deoD"
                     /locus_tag="ACHJ12_004153"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4991862.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3046 a         3240 c         3430 g         2985 t
ORIGIN      
        1 ggacttggta aaaacggtct taagtacagg agtgtgaagc acctggttgg acgtgcgtag
       61 ccggaatgca tattcgtgaa gttgggtaat gagagttgaa cggtggcagt gagaatggag
      121 caggaaaaaa tcagatgcgg aaagcggaaa tacacagcgg gtgcgatgtg cttcatgctg
      181 tttgtatggc tgggtggtgt ggtaacactc tgtgtacttg ctgcactgat aacacaataa
      241 aagaggggag ctgccagctc cccgtgctga tgtcaccgga taaaccagtc atccgctgtt
      301 tcccagactt cctgcagcgt ttcctgtaca aattcttgcg cggattcttt atccacggct
      361 cttaaaattg tgagtccatc attactggca gatttcacaa tcacatctac atcatcataa
      421 cgcttaccga cacgtcgtag catttcctgt tgcagggcag gaacagagcc ttttggcatt
      481 ttgcagattt tttctttagc gatacagatc tcaatacgca taacacctcc tgtcactgtg
      541 tttgtataca gtgttatttt taactgtatg aataaacagt gtcaatagag caaagattgc
      601 tgcatataca catgttcttt ggctacaaat acacctactg atcatgttct gctatgctct
      661 gatacctcca aaatgtacaa tcagcggcat gaatttttct ttgtacatat gattgtacat
      721 aatcaaagag acaacaatta tgacaagcct tttggccgca gaggtagcga aaagaagaac
      781 ctttgcaatt atttctcacc cggatgccgg taagactacc atcaccgaga aggtgctgct
      841 gttcggacag gccattcaga ccgccggtac agtaaaaggc cgtggttcca accagcacgc
      901 taagtcggac tggatggaga tggaaaagca gcgtgggatc tccattacta cgtctgtgat
      961 gcagtttccg tatcacgatt gcctggttaa cctgctcgac accccggggc acgaagactt
     1021 ctcggaagat acctatcgta ccctgacggc ggtggactgc tgcctgatgg ttatcgacgc
     1081 tgcaaaaggt gttgaagatc gtacccgtaa gctgatggaa gttacccgtc tgcgcgacac
     1141 gccgatcctc acctttatga acaaacttga ccgtgatatc cgcgacccga tggaactgct
     1201 cgatgaagtt gagaacgagc tgaaaatcgg ctgcgcaccg atcacctggc cgattggctg
     1261 cggcaagctg tttaaaggcg tttaccacct ttataaagac gaaacctatc tctatcagag
     1321 cggtaaaggc cacacgattc aggaagtccg tattgttaaa gggctgaata acccggatct
     1381 cgacgctgcg gttggtgaag atctggcaca gcagctacgt gacgaactgg aactggtgaa
     1441 aggcgcgtct aacgagttcg acaaagaact gttccttgcg ggcgaaatca ctccggtatt
     1501 cttcggtact gcgctgggta acttcggtgt tgatcatatg ctggatggcc tggtggagtg
     1561 ggctcctgcg ccgatgccgc gtcagactga tacccgtacc gtagaggcga gcgaagacaa
     1621 atttaccggc ttcatattta aaattcaggc caacatggac ccgaaacacc gcgaccgcgt
     1681 ggcgtttatg cgcgtagtat ccggtaaata tgaaaaaggc atgaagctgc gccaggtgcg
     1741 tactgcgaaa gatgtggtta tctccgacgc gctgaccttt atggcgggcg accgttcgca
     1801 cgttgaagaa gcgtatccgg gcgatatcct cggcctgcac aaccacggca ctattcagat
     1861 cggcgacacc tttacccagg gtgagatgat gaagttcacc ggtattccga acttcgcacc
     1921 agaactgttc cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc tgctcaaagg
     1981 gctggtacag ctttccgaag agggcgcggt gcaggtgttc cgtccgatct ccaacaacga
     2041 tttgatcgtt ggtgctgttg gtgtgctgca gtttgatgtg gtggtatcgc gcctgaagag
     2101 cgaatacaac gttgaagcag tatatgaatc agtcaacgtt gccactgccc gctgggtaga
     2161 atgtgcggac gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc aactggcgct
     2221 tgatggcggc gataacctcg cttacatcgc taccagcatg gtcaacctgc gcctggcgca
     2281 ggaacgttat cccgacgttc agttccacca gacccgcgag cattaattct tgcctccagg
     2341 gcgcggtagc cgctgcgccc tgtcaatttc ccttccttat tagccgctta cggaatgttc
     2401 ttaaaacatt cacttttgct tatgttttcg ctgatatccc gagcggtttc aaaattgtga
     2461 actatattta acaaagtaat gacatttctg acggcgttaa ataccgttca atgcgtagat
     2521 atcagtatct aaagccgtcg attgtcattc taccgatatt aataactgat tcagaggctg
     2581 taatggtcgt tattcatcac ccatcgcttt tgtgatggcg accattgact tctgtagagg
     2641 gtgaagtctc tccctattca gcaatgcaac ctcgtgttgc caggctcaaa ttacgagcaa
     2701 acatacagga ataaatcgat gactatgaca agactgaaga tttcgaaaac tctgctggct
     2761 gtaatgttga cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa cgcacagact
     2821 accaatgaaa gcgcagggca aaaagtcgat agctctatga ataaagtcgg taatttcatg
     2881 gatgacagcg ccatcaccgc gaaagtgaag gcggcgctgg tggatcatga caacatcaag
     2941 agcaccgata tctctgtcaa aaccgatcaa aaagtcgtga ccctgagcgg tttcgttgaa
     3001 agccaggctc aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg ggtgacctct
     3061 gtcagcgaca aactgcacgt tcgcgacgct aaagaaggct cggtgaaggg ctacgcgggt
     3121 gacaccgcca ccaccagtga aatcaaagcc aaactgctgg cggacgatat cgtcccttcc
     3181 cgtcatgtga aagttgaaac caccgacggc gtggttcagc tctccggtac cgtcgattcc
     3241 caggcacaga gcgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt gaaaagcgtt
     3301 aaaaatgatc tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact ttgtcgggag
     3361 gcgcgatgtg caccacacta aaaatatcgc attgagtgat tttcacgctc atatcaagcg
     3421 gtgacattaa ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat atttctggtt
     3481 atcgcgttaa tcgccgccgc acttgggttt ggtggtctgg ccggtaccgc tgcaggcgca
     3541 gctaaaattg tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt catgggccga
     3601 aaacgaccct agatttcaga ccattaagaa aattttatcc aaagccagtc cagcggactg
     3661 gcttttgcgg ttttagcgaa taataaattg cgttactttg tcattctttc acaacaaaaa
     3721 caggaaggca gaggtggggc agcgaatacc tgtaacgctt ggtaatattg cgccgttgtc
     3781 gctaaggccg ttccagcctg gacgaatagc tctggtgtgc gaaggcggcg gacagcgtgg
     3841 aattttcacg gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc ctttcgatct
     3901 ttatctcggc acatctgccg gggcgcagaa cctctcggcg tatatctgca atcagcccgg
     3961 ttacgcgcgc aaagtcatca tgcgctatac cacaaaacgc gaatttttcg atccattgcg
     4021 ctttgtccgt ggaggaaatc ttatcgatct cgactggctg gtggaggcca ccgcaagtca
     4081 gatgccgttg caaatggata ccgccgcgcg gttgtttgac agcggcaaat cgttttatat
     4141 gtgcgcctgt cgtcaggatg actacgcgcc gaattacttt ttaccaacca aacaaaactg
     4201 gctggatgtg attcgcgcct ccagtgcgat acctggcttt tatcgtagcg gagtgtcgct
     4261 ggaaggcatt aactacctgg atggcgggat cagtgatgcg attccggtta aagaggcggc
     4321 aaggcagggc gctaaaacgt tggtcgtcat ccgcactgtg ccgtcacaaa tgtactacac
     4381 gccgcagtgg ttcaaacgca tggaacgctg gctgggtgac agtagcctgc agccgctggt
     4441 caatctggtg cagcatcatg aaaccagcta tcgtgacatt cagcaattta ttgagaaacc
     4501 accgggcaag ctgcggatat tcgaaattta tccaccgaag ccattacata gtatcgcgct
     4561 tggcagtcgg attccggcgc tgcgtgaaga ctataaactt gggcgtttat gcggtcgtta
     4621 tttcctcgcc acggttggca agctattaac gcttacccgc catctggtgc cagtggtgac
     4681 gccggaatcg atcgttattc cgcctgcgcc agtcgccaac gatacgctgg ttgccgaagt
     4741 gagcgatgct ccgcaggcga acgacccgac atttaacaat gaggatctgg cttgatttgc
     4801 cgttttatcg atacccactg ccattttgat ttcccgccgt ttagtggcga tgaagaggcc
     4861 agcctgcaac gcgcggcaca agcgggcgta ggcaagatca ttgttccggc gacagaggcg
     4921 gaaaattttg cccgcgtgca ggcgttagcg gaaaaatatc aaccgcttta tgccgcattg
     4981 gggttgcatc cggggatgtt ggaaaaacat agcgatgtgt ctcttgatca gctacagcag
     5041 gcgctggaaa ggcgtccggc gaaggtggtg gcggtggggg agatcggtct ggatctcttc
     5101 ggcgacgatc cgcaatttga gaggcagcag tggttactcg acgaacagct gaaactggcg
     5161 aaacgctacg atctgccggt gatccttcat tcccggcgca cacacgacaa actggcgatg
     5221 catcttaaac gccacgattt atcgcgcact ggcgtggttc acggtttttc cggcagcctg
     5281 caacaggccg aacggtttgt acagctgggc tacaaaatcg gcgtaggcgg tactatcacc
     5341 tatccacgcg ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc atcgttgttg
     5401 ctggaaaccg acgcgccgga tatgccgctc aatggttttc aggggcaggc taaccgcccg
     5461 gaacaggccg cccgggtatt tgccgtgctg tgcgagttgc gctcggaacc ggcggatgag
     5521 attgccgaag tgttgcttaa taatacgtat gcggtgttta gcgttagtgg gtagtttgcc
     5581 cttcgtaggc agcatccggc agtcggcgca taatgcctga tgcgacgctt gtcgcgtctt
     5641 atcatgccta caggtttgtg ccgaaccgta ggccgtatcc ggcatgtcac aaatagagcg
     5701 ccggaaatat caaccggctc accccgcgca cctttaacgc atcagccaac ggctcaacgt
     5761 cttccggcgt ggcgctcgcc cagctttgcg cctcgccata cacgccgtgg gcatgaaacg
     5821 cattcaggcg aaccggaaca tcgccaagtc tcttgataaa cgtcgccagc tcatcgatgt
     5881 gatgcaaata atccacctgg tctggaatta ccagcaaacg cagttccgcc agcttaccgc
     5941 gctctgccag caaacagatg ctgcgcttaa tctgctgatt atcgcgtccg gttaattgct
     6001 gatgacattc gctcccccac gctttgagat cgagcatcgc gccgtcgcac accgggagta
     6061 atttttccca gccggtttcg ctcaacatac cgttactgtc cacgaggcag gtgaggtggc
     6121 gcagttgcgg atcgtttttg atggctgtaa acagcgccac cacaaacggc agttgggtcg
     6181 tggcttcgcc gccgcttacc gttatccctt caataaacag caccgctttg cggacatggc
     6241 taagcacttc gtccacgctc atggattgcg ccattggcgt ggcatgttgc ggacacctct
     6301 tcaggcaggt atcacactgc tcgcaaacca cagcgttcca caccactttg ccgtcaacaa
     6361 tctgcaacgc ctgatgcgga cactgtggca cgcactcccc acagtcattg caacgtccca
     6421 tcgtccacgg attgtgacag tttttgcagc gcagattgca gccctgcaaa aacagagcca
     6481 gacgactgcc tggcccgtca acgcaggaga aggggataat cttactgact aaagcgcatc
     6541 tgctgttcat ggcttatcac gcgcggctgg cgttccagaa tacgagtgtt gcgtgcggct
     6601 tcttcgccca gccaggtggt gttggtgcgt gaaccttcgg cgcgatattt ttctaagtcc
     6661 gacaaacgca ccatataacc ggtaacgcga accagatcgt taccgctgac attggcggta
     6721 aattcacgca ttccggcttt aaaggcaccg aggcaaagct gtaccagtgc ctgcgggtta
     6781 cgtttgatgg tttcgtcgag cgtcagaatg tcgctgatgc cggaataata ataagcatga
     6841 tgcggggcga cagtttgcag atgggtgatc ggatctggct catcgccata cggcaaacgc
     6901 gcgcccggcg tggtgccgat atcggaactg atccccgact gtgcgtgtaa catggcgcgt
     6961 ttttgccagc catatttcac gggggtattg gcgacaaact ccgccagttg cgcgctgatg
     7021 cgataaccca cttcatttgc ggcggcttct ttaccgtagc gcgcggcaat cccttctttc
     7081 ttacacagca agttaaccgc ttccgccagc ccatacatgc caaacattgg cacaaaacgt
     7141 tcagggttaa tcagcccttc tttcaccagg aagctattct caaagaagtg tgattgttga
     7201 tagaggaatt cacaccgcgc atcgatgatg gcgatctgct gctggcagta gtgcggtaga
     7261 gtgcgcgtaa agaagtcatc cagcgattcg ctgcgctcgg caatggcttt caggttaagg
     7321 cgtaccagcg tgctgccacc acccgccagc ggcagtgagt tgtaacagct cacaatcccg
     7381 tagccccctt ttgtgaaaat tttatcatgc accggaccgt tggcgatgtg cggtttgcta
     7441 cattcacaga tgttcttcgc cacttccagc agcaggtcat caggggtgat ttcaggatcg
     7501 tagataaagg tcaggttcgg tgaaacctgc ttcaactctg cgtctgcacg taagatcgcg
     7561 cgggtaatgg gcgaatcaga cgggccgata ttggcgtgca taaaggcgtc tggcagggtt
     7621 ctgtcgaggt aacgccagaa acgttttatt cgaacatcga tctcgtcttg tgttagaatt
     7681 ataacatacg gttgcaacaa cgcatccagt tgccccaggt agaccggcat cgatgtgacc
     7741 gacggtacgt ggtggtaaag aatggtcagc agagagagtg cgtcatcaag atctttcgcg
     7801 ccttccagct ccagccattc ggaaccgttc gccagaaaac gggcgtaatc gggtaagaca
     7861 tagcgcggtt tgtacggcgc atgaccttca aacatatcgc agattacacc ctcatccagc
     7921 gcgcggcggg cttcggcagg aagctgcgga taaggcagat tgttttctgc ttccagagcc
     7981 agaaaatggc gcttctgctc cgggctaagc actgggctgg tgacaatttg ctggcaacgt
     8041 tgttgcagtg cattttcatg agaagtgggc atcttctttt ccttttatgc cgaaggtgat
     8101 gcgccattgt aagaagtttc gtgatgttca ctttgatcct gatgcgtttg ccaccactga
     8161 cgcattcatt tgaaagtgaa ttatttgaac cagatcgcat tacagtgatg caaacttgta
     8221 agtagatttc cttaattgtg atgtatatcg aagtgtgttg cggagtagat gttagaatac
     8281 taacaaactc gcaaggtgaa ttttattggc gacaagccag gagaatgaaa tgactgatct
     8341 gaaagcaagc agcctgcgtg cactgaaatt gatggacctg accaccctga atgacgacga
     8401 caccgacgag aaagtaattg ctctgtgtca tcaggccaaa actccggtcg gcaataccgc
     8461 cgctatctgt atctatcctc gctttatccc gattgctcgc aaaacgctga aagagcaggg
     8521 caccccggaa atccgaatcg ctacggtaac caacttccca cacggtaacg atgacatcga
     8581 aatcgcgctg gcagaaaccc gtgcggcaat cgcctacggt gctgatgaag ttgacgttgt
     8641 gttcccgtac cgcgcgctga tggcgggtaa cgagcaggtt ggttttgacc tggtgaaagc
     8701 ctgtaaagag gcttgcgcgg cagcgaatgt actgctgaaa gtgatcatcg aaaccggcga
     8761 actgaaagac gaagcgctga tccgtaaagc gtctgaaatc tccatcaaag cgggtgcgga
     8821 cttcatcaaa acctctaccg gtaaagtggc tgtgaacgcg acgccggaaa gcgcgcgcat
     8881 catgatggaa gtgatccgtg atatgggcgt agaaaaaacc gttggtttca aaccagcggg
     8941 cggcgtgcgt actgcggaag atgcgcagaa atatctcgcc atcgcagatg aactgttcgg
     9001 tgctgactgg gcagatgcgc gtcactaccg ctttggtgct tccagcctgc tggcaagcct
     9061 gttgaaagcg ctgggccacg gtgacggtaa gagcgccagc agctactaag taagatgctt
     9121 tacgcctgat gcgctgcgct tatcaggcct acgagacgta tctacccgta ggccggataa
     9181 ggcgtagacg catccggcaa aagccgcctc atactctttt cctcgggagg ttaccttgtt
     9241 tctcgcacaa gaaattattc gtaaaaaacg tgatggtcat gcgctaagcg atgaagaaat
     9301 tcgtttcttt atcaacggta ttcgcgacaa cactatctcc gaagggcaga ttgccgccct
     9361 cgcgatgacc attttcttcc acgatatgac aatgcctgag cgtgtctcgc tgaccatggc
     9421 gatgcgagat tcaggaaccg ttctcgactg gaaaagcctg catctgaatg gcccgattgt
     9481 tgataaacac tccaccggcg gcgtcggcga tgtgacttcg ctgatgttgg ggccggtggt
     9541 cgcagcctgc ggcggctata ttccgatgat ctccggtcgc ggcctcggtc atactggcgg
     9601 tacgctcgac aaactggaat ccatccctgg cttcgacatt ttcccggatg acaaccgttt
     9661 ccgcgaaatt attaaagacg tcggcgtagc gattatcggc cagaccagct cactggctcc
     9721 ggcggataaa cgtttctacg cgacccgtga tattaccgca accgtggact ccatcccgct
     9781 gatcactgcc tcgatcctgg cgaagaaact ggcggaaggt ctggatgcgc tggtgatgga
     9841 cgtgaaagtg ggtagcggcg cgtttatgcc gacctacgaa ctctctgaag cccttgccga
     9901 agcgattgtt ggcgtggcta acggcgctgg cgtgcgcacc accgcgctgc tcaccgatat
     9961 gaatcaggta ctggcctcca gtgcaggtaa cgcggttgaa gttcgtgaag cggtgcagtt
    10021 cctgacgggt gaatatcgta acccgcgtct gtttgatgtc acgatggcgc tgtgcgtgga
    10081 gatgcttatc tccggcaaac tggcgaaaga tgacgccgaa gcgcgcgcga aattgcaagc
    10141 agtgctggac aacggtaaag cggcagaagt ctttggtcgt atggtagcgg cacaaaaagg
    10201 cccaaccgac ttcgttgaga actacgcgaa gtatctgccg acagcgatgc tgacgaaagc
    10261 agtctatgct gataccgaag gttttgtcag tgaaatggat acccgcgcgc tggggatggc
    10321 agtggttgca atgggcggcg gtcgtcgtca ggcatctgac accattgatt acagcgtcgg
    10381 ctttactgat atggcgcgtc tgggcgacca ggtagacggt cagcgtccgc tggcggttat
    10441 ccacgcgaaa gacgaaaaca gctggcagga agcggcgaaa gcggtgaaag cggcaattaa
    10501 acttgccgat aaagcaccgg aaagcacacc aactgtctat cgccgtatca gcgaataacg
    10561 gtatactgat ctgatcattt aaatttgaag cactgagtac ggagaacata tgaaacgtgc
    10621 atttattatg gtgctggact cattcggcat cggcgctaca gaagatgcag aacgctttgg
    10681 tgacgtcggg gctgacaccc tgggtcatat cgcagaagct tgtgctaaag gcgaagctga
    10741 taacggtcgt aaaggcccgc tcaatctgcc aaatctgacc cgtctggggc tggcgaaagc
    10801 acacgaaggt tctaccggtt tcattccggc gggaatggac ggcaacgctg aagttatcgg
    10861 cgcgtacgca tgggcgcacg aaatgtcatc cggtaaagat accccgtctg gtcactggga
    10921 aatcgccggt gtcccggttc tgtttgagtg gggatacttc tccgatcacg aaaacagctt
    10981 cccgcaagag ctgctggata aactggttga acgcgctaac ctgcctggtt atctcggtaa
    11041 ctgccactct tccggtacgg tcattctgga tcagctgggc gaagagcaca tgaaaaccgg
    11101 caagccgatt ttctatacct ccgctgactc cgtgttccag attgcctgcc acgaagaaac
    11161 ctttggcctg gataaactct acgaactgtg cgaaatcgcc cgtgaagagc tgaccaacgg
    11221 cggctacaac atcggtcgtg ttatcgctcg tccgtttatt ggcgacaaag ccggtaactt
    11281 ccagcgtacc ggtaaccgtc acgacctggc tgttgagccg ccagcaccga ccgtgctgca
    11341 gaaactggtt gatgaaaaac acggtcaggt ggtttctgtc ggtaaaattg cagacatcta
    11401 cgccaactgc ggtatcacca agaaagtgaa agcaactggc ctggacgcgc tgtttgacgc
    11461 caccatcaaa gagatgaaag aagcgggtga taacaccatc gtcttcacca acttcgttga
    11521 cttcgactct tcctggggcc accgtcgcga cgtcgcaggt tatgcggcgg gtctggaact
    11581 gttcgaccgc cgtctgccgg agctgatgtc tctgctgcgc gatgacgaca tcctgatcct
    11641 cactgctgac cacggttgtg atccgacctg gaccggtact gatcacacgc gtgaacacat
    11701 tccggtactg gtttacggcc cgaaagtaaa accgggctca ctgggtcacc gtgaaacctt
    11761 cgcggatatc ggccagactc tggcaaaata ttttggtact tctgatatgg aatatggcaa
    11821 agccatgttc tgataatggg tgcggcttga tgccctcacc ccgaccctct ccctcaggga
    11881 gagggtgaaa acatgtagat aaggataaaa caatggctac cccacacatt aatgcagaaa
    11941 tgggcgattt cgctgacgta gttttgatgc caggcgaccc gctgcgtgca aagtatattg
    12001 ctgaaacttt ccttgaagat gcccgtgaag tgaacaacgt tcgcggtatg ctgggcttca
    12061 ccggtactta taaaggccgc aaaatttccg taatgggtca cggtatgggt atcccgtcct
    12121 gctccatcta caccaaagaa ctgatcaccg atttcggcgt gaagaaaatt atccgcgtgg
    12181 gttcctgtgg cgcagttctg ccgcacgtaa aactgcgcga cgtcgttatc ggtatgggtg
    12241 cctgcaccga ttccaaagtt aaccgcatcc gttttaaaga tcatgacttt gccgctatcg
    12301 ctgactttga catggtgcgt aacgcggtag acgcggctaa agcactgggc attgatgctc
    12361 gcgttggtaa cctgttctcc gctgacctgt tctactctcc ggacggcgaa atgttcgacg
    12421 tgatggaaaa atacggcatc ctcggcgtgg aaatggaagc ggctggtatc tacggcgtcg
    12481 ctgcagaatt tggcgcgaaa gccctgacca tctgtaccgt gtctgaccac atccgcactc
    12541 acgagcagac cactgccgct gagcgtcaga ccaccttcaa cgacatgatc aaaatcgcac
    12601 tggaatccgt tctgctgggc gataaagagt aattgtgttt cgctgaaagg cgattgtcgt
    12661 gtgaagccgg agtgggagac tgctccggct ttttagtatc t
//
LOCUS       DAZFFI010000127        12613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFFI010000127 DAZFFI010000000
VERSION     DAZFFI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.127"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1517
                     /gene="caiT"
                     /locus_tag="ACHJ12_004154"
     CDS             3..1517
                     /gene="caiT"
                     /locus_tag="ACHJ12_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4991863.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1548..2690
                     /gene="caiA"
                     /locus_tag="ACHJ12_004155"
     CDS             1548..2690
                     /gene="caiA"
                     /locus_tag="ACHJ12_004155"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4991864.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2819..4036
                     /gene="caiB"
                     /locus_tag="ACHJ12_004156"
     CDS             2819..4036
                     /gene="caiB"
                     /locus_tag="ACHJ12_004156"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4991865.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4110..5663
                     /gene="caiC"
                     /locus_tag="ACHJ12_004157"
     CDS             4110..5663
                     /gene="caiC"
                     /locus_tag="ACHJ12_004157"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4991866.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5772..6557
                     /gene="caiD"
                     /locus_tag="ACHJ12_004158"
     CDS             5772..6557
                     /gene="caiD"
                     /locus_tag="ACHJ12_004158"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4991867.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6563..7153
                     /gene="caiE"
                     /locus_tag="ACHJ12_004159"
     CDS             6563..7153
                     /gene="caiE"
                     /locus_tag="ACHJ12_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4991868.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7239..7634)
                     /gene="caiF"
                     /locus_tag="ACHJ12_004160"
     CDS             complement(7239..7634)
                     /gene="caiF"
                     /locus_tag="ACHJ12_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4991869.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7895..11116)
                     /gene="carB"
                     /locus_tag="ACHJ12_004161"
     CDS             complement(7895..11116)
                     /gene="carB"
                     /locus_tag="ACHJ12_004161"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4991870.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11134..12282)
                     /gene="carA"
                     /locus_tag="ACHJ12_004162"
     CDS             complement(11134..12282)
                     /gene="carA"
                     /locus_tag="ACHJ12_004162"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4991871.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2942 a         3288 c         3356 g         3027 t
ORIGIN      
        1 tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa
       61 taatcgtcgg catactttgt tggcttacag tcagagatct ggatgcagcg aatgtcgtta
      121 ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg
      181 tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag
      241 gtaacgaacc acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta
      301 cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc
      361 cgtttggctt agaaccgaac tcgacagggg cgaaagagtt ggggctggct tacagcttgt
      421 tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact
      481 tcttctttgt ccgcaaaatg gaagtgattc gccccagctc gacactggtg ccgctggtag
      541 gtgaaaaaca cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct
      601 tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc
      661 aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga
      721 ttatcctcaa cgccatttgc gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg
      781 acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca
      841 gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgaa
      901 tgttgttcta taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct
      961 actgggcatg gtgggtgatt tacgccatcc agatgagtat cttcctcgcc cgcatctccc
     1021 gtggtcgtac tgtgcgtgaa ctgtgcttcg gcatggtgat ggggctgaca gcatccacct
     1081 ggatcctgtg gaccgtgctc ggtagtaaca ctctgctgtt gatggataaa aacatcatca
     1141 acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa acctgggccg
     1201 ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg
     1261 ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg
     1321 atggtgaaga accaccgctg ctggtgcgta tcggttggtc aattctggtt ggcattatcg
     1381 gtattgttct gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag
     1441 gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac
     1501 agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt
     1561 taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact
     1621 gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag
     1681 cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg
     1741 cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct
     1801 atgtgctgta ccagttgccg ggcgggttca acaccttcct gcgcgaaggc acacaagagc
     1861 agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta
     1921 ccgaaccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg
     1981 gtaagattta tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca
     2041 tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg
     2101 ttgatatgag caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg
     2161 atagctgctg tgaaatcacc tttgacgacg tggaactgga cgagaaagac atgttcggtc
     2221 gggaaggtaa cggctttaac cgcgtcaaag aagagttcga tcatgaacgt ttcctggtag
     2281 ccctcaccaa ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc
     2341 agcgcgtgca gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc
     2401 acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag
     2461 acaacggcac catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg
     2521 catttgaagt tgtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc
     2581 accgcatcag ccgcttctgg cgtgatctgc gtgtagaccg cgtctccggg ggatctgacg
     2641 aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc
     2701 tgcctgatgc gacgctaatg cgtcttatca ggcctacggg agcacgaatg taggccggat
     2761 aaagcgttta cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat
     2821 ggatcatcta cccatgccga aattcgggcc gttggccgga ttgcgcgttg tcttctccgg
     2881 tatcgaaatc gccgggccgt tcgccgggca aatgttcgca gaatggggtg cggaagttat
     2941 ctggatcgag aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcagct
     3001 ctcccgccgc aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc
     3061 gtttctgaaa ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt
     3121 tgcccgtcgt ggcattaccg atgaagtact gtggcagcac aacccgaaac tggttatcgc
     3181 tcacctgtcc ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa
     3241 caccatcgcc caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat
     3301 gcctgccttc ccgtataccg ccgattactt ttctggcctg accgccacca cggcggcgct
     3361 ggcagcactg cataaagtgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta
     3421 tgaagtgatg ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat
     3481 gtgcccgcgc atgagcaaag gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg
     3541 tgccgacggc tacatcgtga tggaactggt gggcattacc caaattgaag agtgctttaa
     3601 agatattggc ctcgcacatc tactcagtac gccggaaatc ccggaaggca ctcagcttat
     3661 ccaccgtatc gaatgccctt acggcccact ggtagaagaa aaactcgatg cctggctggc
     3721 ggcacatacc atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa
     3781 agtactgacc gtaccggaac tggaaagcaa tccacagtat gttgcccgtg aatcaatcac
     3841 tcagtggcaa acgatggatg gtcgcacctg caaagggccg aacatcatgc cgaaattcaa
     3901 aaataacccc ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat
     3961 tttgaaaaat atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc
     4021 caaagttgag gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata
     4081 acaaacgaac aaaaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc
     4141 aaatgtggga cgatcttgcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca
     4201 gcggcggagt cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg
     4261 caaacctgtt ttatacgctg gggattcgca aaggcgacaa ggttgcacta catctcgaca
     4321 actgcccgga atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc
     4381 cgattaacgc ccgcctgtta cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt
     4441 gcctgctggt gaccagtgcg caattctatc ctatgtatca acagattcag caggaagatg
     4501 ccactcaatt gcggcacatt tgcctgacag atgtggcact tcccgctgat gatggcgtga
     4561 gttcgtttac tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat
     4621 cgactgacga tacggcggaa attctcttca cctccggcac cacctcccga ccgaaaggtg
     4681 tggtgattac ccattacaac ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac
     4741 tgcgtgacga tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta
     4801 ctgcggcgat ggcggcgttt tctgccgggg ccacctttgt gctggtcgag aaatacagcg
     4861 cccgcgcctt ctggggacag gtgcagaagt accgcgccac cattaccgaa tgtattccga
     4921 tgatgatccg tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc
     4981 gggaagtgat gttttatctc aacttgtcgg agcaggaaaa agatgcgttt tgtgaacgct
     5041 tcggcgttcg cttgctgacg tcttatggga tgacggaaac cattgtgggc attatcggcg
     5101 atcgccctgg cgataaacga cgctggccgt cgattggtcg ggcggggttt tgctacgaag
     5161 cggagatccg cgacgatcac aatcgcccgc tcccggcagg tgagatcggt gaaatctgta
     5221 ttaaaggcgt accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg
     5281 cgaaagtgct ggaagccgac ggctggctac ataccggcga taccggatac tgcgacgaag
     5341 agggcttttt ttatttcgtc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg
     5401 tctcctgcgt ggagctggaa aatatcatcg ccacccatcc aaaaattcag gatatcgtgg
     5461 ttgtgggtat taaagattcg attcgcgatg aagccatcaa agcatttgtg gtgctgaatg
     5521 aaggtgaaac attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat
     5581 ttaaagtgcc ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa
     5641 taattagaaa gaatctgaaa taaatgaaac agcagggaac taccctgcca gctaataatc
     5701 acgctataaa acaatatgca ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat
     5761 ggagaaaagg aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta
     5821 cccttgatcg tccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag
     5881 tatttctaaa tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag
     5941 agaagttctt ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg
     6001 ctgactttgg cccgggtgga tttgcgggat taaccgaaat tttcaatctc gacaaaccgg
     6061 ttatcgcagc tgtgaacggc tatgcctttg gcggcggttt cgaactggcg ctggcggcag
     6121 attttattgt ttgtgccgat aacgccagct tcgccctgcc ggaagccaag ctgggcatcg
     6181 ttcctgacag cggcggcgtg ctgcgtctac cgaagatcct gccgcctgcc atcgtcaatg
     6241 aaatggtgat gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg gggatagtca
     6301 accgcgtggt tagccaggcg gaactgatgg ataacgcccg cgaactggcg cagcagctgg
     6361 ttaacagcgc cccgctggcg attgcggcgc tgaaagagat cttccgcacc accagcgaaa
     6421 tgccggtaga agaagcgtat cgctatattc gcagcggcgt gttgaaacac tatccgtcgg
     6481 ttctgcattc ggaggatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccgg
     6541 tgtggaaagg acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggtggt
     6601 tcacccgacg gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc
     6661 cggtgtctac atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca
     6721 agcgggagcc aatattcagg atggctgcat tatgcatggc tactgcgaca ctgacaccat
     6781 cgttggggaa aacggccata tcgggcacgg agcgatcctg catggttgtg tgattggtcg
     6841 cgatgcattg gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag
     6901 cattgttgcc gccatgagct ttgtcaaagc aggctttcac ggcgagaaac gccagttgtt
     6961 gatgggtacg ccagcccgcg ctgtacgcag tgttagtgac gacgagttac actggaaacg
     7021 gttgaatacc aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tacatgaaac
     7081 acagccactg aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac
     7141 gcctaaacgg tgatcttgcc ggatgcgacc tggttgctgg ataagatgct acaacatcgc
     7201 atctggcaag caggactccc ggtatcaacg agataaaatt aacgacgcat actctttgac
     7261 aacatcatct gccacttctg ctcgcggtta agttccgtga gcggaacggc aggaatacgc
     7321 ggcgtctttc ttacgccgac taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt
     7381 tgctcatcga tatcgaccac ttttaccagt cgctggcact ggcatccccg tccttccagc
     7441 ttattaggga tcatcttaac ttcgcagctt atttctgcga cttccgacag aatataagtc
     7501 agggtattaa ccgccttgct gtgttcaata tcgaaatgaa tagagatctc tcttgctatc
     7561 acccaccgat tttcagccat catccattcg gcgattaaca agtagagtgg tttttcaaca
     7621 tatccttcac acatacgaac tccagtttca caaattaaaa gtctggctat gactcgtttt
     7681 gaagcaatta aacaaggcca tattaaaatt gtggatatat cgatagtatg caacttcacc
     7741 gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc cccaaaagat
     7801 ttacttaatg ggcaaaaaaa acggtcctca tcagagaacc gtattactta ttcgatcaaa
     7861 ttagcggatg aaaaatatcc gccatgacac gctattattt gatctgcgcg tgcatttcct
     7921 gcaccgaaat tactttttcg gtcgcgtcgg cattcagcgc catcgcggta gcgaaaccac
     7981 cgttcagggt ggtgtcataa tgcactttat attgcagcgc actgcgacgg atcacgcggg
     8041 agtcttcaat cgcgcgacgg cctgaagtgg tgttgatgat gtaggtatat tcgccattct
     8101 tgatgcggtc ctgaatgtgc ggacggcctt catgcacctt gttcaccaga cgcggattaa
     8161 tgcccgcttc gcccagcaca atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt
     8221 tcagcagttt tgccgccagg tccaccacgc gttctttatc gccttcgcgc acggaaagca
     8281 acgcacgacc gtgtttcttc atggtggagt tgctgcccag ctgcgctttg gcaaacgctt
     8341 cagcgaaggt gcggcccacg cccatgactt ccccggtaga gcgcatttct ggccctaaca
     8401 gcgggtcaac acccgggaat ttattgaacg gcagcaccac ttctttcacc gagtagtacg
     8461 gcgggataac ttctttggtt acgccctgct cagccagcga tttgccagcc atcacacgcg
     8521 ccgccacttt tgccagcgga acgccggtgg ctttggagac gaacggaacg gtacgcgccg
     8581 cacgcgggtt aacttcaatc aggtagactt cgttgttttt caccgcaaac tgcacgttca
     8641 tcagaccgcg cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc atcacatcct
     8701 gaatttcctg acttaaggtg taggctggca gagaacatgc ggagtcaccg gagtgcacgc
     8761 ccgcctgctc gatgtgctcc ataatgccgc caatcagcac catttcaccg tcgcagatgg
     8821 catccacgtc aacttccacc gcgtcatcga ggaagtgatc cagcaacact ggcgcatcgt
     8881 tagacacgct gaccgccgtc tggaagtagc gacgcaggtc agcttcgtca tagacgattt
     8941 ccatcgcccg accgccgaga acgtaagacg gacgtaccac cagcgggtag ccaatctctt
     9001 tcgccttctc taccgccatt tcaatagcgg taacggtggc gttcgccggt tgtttcagtt
     9061 tcagacgctc aaccgcatgc tggaagcgtt cacggtcttc tgcacggtcg atagcatccg
     9121 ggctggtgcc gataaccggt acgccagcgg cttccagtgc acgcgccagt ttcagcgggg
     9181 tctgaccgcc gtactgaacg ataacgcctt tcggcttctc aatacgcacg atttctagca
     9241 catcttccag agttaccggc tcgaagtaga ggcgatcgga agtgtcgtag tcggtggaga
     9301 cggtttccgg gttacagtta accataatgg tttcgtaacc gtcttcgcgc agcgccagcg
     9361 aggcgtgtac acagcagtag tcgaattcga taccctgacc gatacggttc gggccgccgc
     9421 cgagaaccat gattttttca cggtcggtag acggattcgc ttcgcactct tcttcataag
     9481 tggagtacat gtaagcggtg tcggtggcga actctgccgc acaggtatcc acgcgcttat
     9541 aaaccgggtg caggtcatac tggtcgcgca gcttgcggat ttccgcttcg cgcacgcccg
     9601 ccagttttgc cagacgcgca tcggcaaagc ctttgcgttt cagctggcgc aggaattcag
     9661 cgttcaggcc agtgatgccc acttccgcta ctttctcttc cagacgcacc agttcttcaa
     9721 tctgtaccag gaaccaacgg tcaatattgg tcaggttgaa cacgccgtcc acagacaggc
     9781 ccgcacggaa ggcatcggcg atgtaccaga tacgctctgc gccagcatct ttcagttcgc
     9841 gacggatttt ggttaacgct tccgggtcat cgaggctcac tttcgggtcg aatccagtcg
     9901 caccgacttc caggccgcgc agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg
     9961 ccatcacttc gccaaccgat ttcatctgag tggtcagacg gtcgttagca ccagcgaatt
    10021 tttcgaagtt gaagcgagga attttagtaa ccacatagtc gatggacggc tcgaaggagg
    10081 ccggagtacg tccgccagtg atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca
    10141 gtttcgccgc cactttagca atcgggaaac cggtcgcttt cgacgccagc gccgaagaac
    10201 gggacacgcg tgggttcatt tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa
    10261 actggacatt ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt
    10321 tacgcatgat ttgatattct ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt
    10381 caccggtatg gatgcccatc gcatcaaagt tttcgataga gcagacgatg atgcagttgt
    10441 cgtttttatc acgcaccact tccatctcgt actctttcca gccgatcagc gactcatcaa
    10501 tcagcagctc tttggttggg gagagatcca gaccgcgggc gcaaatttct tcaaactctt
    10561 cgcggttata agcgataccg ccgccgctac cgcccatggt aaaggatggg cgaataatgc
    10621 acgggaagcc cacgtcagcg gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg
    10681 aacgcgcggt ttccagacca attttcttca tcgctacgtc gaaacgacgg cggtcttctg
    10741 ctttatcaat cgcatcggcg gtcgcaccaa tcatagtcac gccgaactct tccaacacgc
    10801 cctgacgctc cagctccagc gcgcagttca gcgccgtctg accgcccatc gttggcagca
    10861 ccgcgtccgg gcgctctttt tcaataatct tgcgtaccac ttcccagtga atcggctcga
    10921 tgtaggtcgc atcggccatt tccgggtcgg tcatgatggt cgccgggttg gagttcacca
    10981 gaatgacccg gtaaccctct tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa
    11041 actcacacgc ctgaccgata acaatcgggc ccgcacccag aatcaggata ctttttatat
    11101 ctgtacgttt tggcatggct cttttactcc tgattactta gcggttttac ggtactgctc
    11161 aattaactcg ataaagtggt cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc
    11221 agggtgcccc tggaagctga acgccggttt atcggtgcga tgaatgccct gtaacgtacc
    11281 gtcgaacagg gatttatgcg tgacacgcag gtttgctggt aatgttgctt cgtccaccgc
    11341 aaaaccgtgg ttctgggcgg tgatcatcac cacgtttttc tccacatctt taaccggatg
    11401 gttgccgccg tggtgaccaa atttcatttt gacagtcttc gcaccgctcg ccagcgccag
    11461 cagctgatga ccgaggcaga tgccgaagac cggaatatcg gtttcgagga atttctggat
    11521 ggcggtaatg gcgtaatcgc acggggccgg gtcgccagga ccgttggaga ggaagatgcc
    11581 gtctggattc attttcagca catcttccgc agaagtttgc gccggaacga tggtcaggcg
    11641 acagcctctg tccaccaaca tgcgcaggat gttgcgcttg gcaccaaaat cataagccac
    11701 gacgtggaac ggcagctcgt cttctttttt cgcttctggc aggccgccgg tcaacgtcca
    11761 gctcccttgt gtccagctat aggcttctgc ggtggtcact tcttttgcca gatccatgcc
    11821 attcagacct gggaacgcgc gggctttttc taacgccaac gccgcatccg ggttatcgcc
    11881 tgcgataatg cagccattct gtgcgccttt ctcgcgcagt aaacgtgtca gcttacgggt
    11941 atcgatatcg gcaatcgcca cgatgttatg gcgcttcagg taagaagaga ggtcttcggt
    12001 attacggaag ttgctggcaa tcagcggcag gtcgcgaatc accagacctt gtgcatgtac
    12061 ctgagaagat tcttcatcgg cgtcattagt gccgacattg ccaatatggg gataagtaag
    12121 agtaacgatt tgacgagaat aggaaggatc agtgaggatt tcttgataac cggtcattga
    12181 agtattgaaa acgacttccc caaccgccga acctgttgcc cctatggccc gaccgtgaaa
    12241 ctgggttccg tcttccagaa ccaatagcgc tgacttaatc aaaacaccct ccagagaata
    12301 ttcactcact ttatttgcat attaattcac aatgatgacg taaatcaatg caaatctgct
    12361 taccgacgaa tttctggcaa acggcggcat tctggagata tgggcgctaa aagtcaactt
    12421 aatggcggga ttttttatat tattttgtgg ctcatgcatg ttttacaggc attaagcggc
    12481 aaaaagatca tataacctgt tctggaaaac gcttgcgcag caagaagatt gcatgaaata
    12541 aaagcgagta gggaaaagtg gaccaaatgg tcaaaattta cattaaaaca acaaaaaccg
    12601 aagacaaaac atg
//
LOCUS       DAZFFI010000128        12534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFFI010000128 DAZFFI010000000
VERSION     DAZFFI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.128"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..1359)
                     /gene="gltA"
                     /locus_tag="ACHJ12_004163"
     CDS             complement(76..1359)
                     /gene="gltA"
                     /locus_tag="ACHJ12_004163"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4991872.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1565..1672)
                     /locus_tag="ACHJ12_004164"
     CDS             complement(1565..1672)
                     /locus_tag="ACHJ12_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991873.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2053..2457
                     /gene="sdhC"
                     /locus_tag="ACHJ12_004165"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2053..2457
                     /gene="sdhC"
                     /locus_tag="ACHJ12_004165"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4991874.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2451..2798
                     /gene="sdhD"
                     /locus_tag="ACHJ12_004166"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2451..2798
                     /gene="sdhD"
                     /locus_tag="ACHJ12_004166"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4991875.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2798..4564
                     /gene="sdhA"
                     /locus_tag="ACHJ12_004167"
     CDS             2798..4564
                     /gene="sdhA"
                     /locus_tag="ACHJ12_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4991876.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4580..5296
                     /gene="sdhB"
                     /locus_tag="ACHJ12_004168"
     CDS             4580..5296
                     /gene="sdhB"
                     /locus_tag="ACHJ12_004168"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4991877.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5497..8298
                     /gene="sucA"
                     /locus_tag="ACHJ12_004169"
     CDS             5497..8298
                     /gene="sucA"
                     /locus_tag="ACHJ12_004169"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4991878.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8313..9530
                     /gene="odhB"
                     /locus_tag="ACHJ12_004170"
     CDS             8313..9530
                     /gene="odhB"
                     /locus_tag="ACHJ12_004170"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4991879.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9624..10790
                     /gene="sucC"
                     /locus_tag="ACHJ12_004171"
     CDS             9624..10790
                     /gene="sucC"
                     /locus_tag="ACHJ12_004171"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4991880.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10790..11659
                     /gene="sucD"
                     /locus_tag="ACHJ12_004172"
     CDS             10790..11659
                     /gene="sucD"
                     /locus_tag="ACHJ12_004172"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4991881.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11763..12485)
                     /gene="mngR"
                     /locus_tag="ACHJ12_004173"
     CDS             complement(11763..12485)
                     /gene="mngR"
                     /locus_tag="ACHJ12_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO4991882.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3007 a         3198 c         3396 g         2933 t
ORIGIN      
        1 ttgtgtttag gcatataaaa attaacccgc catttgaacg gcgggttaaa atatttacaa
       61 cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt catatcctgt
      121 atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc tccagtgggc
      181 gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag acggaatacc
      241 catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat
      301 aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc
      361 tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg
      421 gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc
      481 tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt
      541 cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa tacctgctgc
      601 gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc
      661 gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg
      721 attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata
      781 ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc
      841 ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat gacgaggatt
      901 gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac
      961 tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct gctcgtggat
     1021 catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc
     1081 accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat
     1141 cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc
     1201 gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg aaccgagagt
     1261 acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt
     1321 gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt
     1381 attacgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt
     1441 tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc
     1501 ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga
     1561 attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac
     1621 tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct
     1681 tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat
     1741 taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata
     1801 tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc tccggaacac
     1861 cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt
     1921 ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg
     1981 aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat
     2041 aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt
     2101 aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc
     2161 gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct gggtaccagc
     2221 ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa
     2281 tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac
     2341 atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa
     2401 atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca
     2461 acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta
     2521 tcgtcctgac gctctacatc atttatatgg tcggtttttt cgctaccagt ggcgagctga
     2581 catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc accctgctgg
     2641 cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg accgactacg
     2701 ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat tgtcgttgct ctggtggttt
     2761 acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt
     2821 tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc aaatttccca
     2881 gagcggtcaa acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc
     2941 tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca
     3001 tatgtacgac accgtaaaag gttcggacta tatcggtgac caggacgcga ttgaatatat
     3061 gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg
     3121 tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg
     3181 cgagcaggcg gcacgcactg cggcagcagc tgaccgtacc ggtcacgcac tgttgcatac
     3241 cctttatcag cagaacctga aaaaccacac cactattttc tccgagtggt atgcgctgga
     3301 tctggtgaaa aaccaggatg gcgcggtggt gggttgtact gcactgtgca tcgaaactgg
     3361 tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg cagggcgtat
     3421 ttatcagtcc accactaacg cccacattaa cactggcgac ggcgtcggca tggctatccg
     3481 cgcaggcgta ccggtgcagg atatggaaat gtggcagttc cacccgaccg gtattgccgg
     3541 tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca
     3601 tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt
     3661 ggtggcgcgt tccatcatga tcgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg
     3721 gccacacgcg aaactgaaac tcgaccacct gggtaaagaa gttctcgaat cccgcctgcc
     3781 gggtatcctg gaactctccc gtaccttcgc tcacgttgat ccggtgaaag agccgattcc
     3841 ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga ccggtcaggc
     3901 gctgacggtg aacgagaaag gcgaagatgt ggttgttccg ggactgtttg ccgttggtga
     3961 aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc tgctggacct
     4021 ggtggtcttt ggtcgtgcag cgggtctgca tctgcaagag tctatcgccg aacagggcgc
     4081 attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgcctga accgctggaa
     4141 caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca
     4201 gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg agcagttgaa
     4261 agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac
     4321 ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg caacggctgt
     4381 ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact tcccggatcg
     4441 tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg
     4501 ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta
     4561 ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt
     4621 tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc gcgacatgat
     4681 gttgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc
     4741 ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc
     4801 ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct
     4861 gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata
     4921 tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt
     4981 acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg
     5041 ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg
     5101 cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct
     5161 cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac agcatcatga actgcgtcag
     5221 tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt cgatgttgtt
     5281 gcaacgtaat gcgtaattaa atgttgatgc cggggaaatc ccccggctat tcaggaaatc
     5341 tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacgt
     5401 agacaagagc tcgcaagtga accccggcac gcacataact gtgcgtggta gtatccacgg
     5461 cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc
     5521 tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa
     5581 gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta
     5641 cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc
     5701 ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac caatgtaaag
     5761 caggttaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat
     5821 ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac
     5881 gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa
     5941 gaaaccatga aactcggcga gctgctggaa gccctcaagc aaacctactg cggcccgatt
     6001 ggtgccgagt atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc
     6061 gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc
     6121 gccgctgaag gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg
     6181 ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac
     6241 agcggcaccc gggaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa cgtgttggtg
     6301 aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa
     6361 cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat
     6421 ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg
     6481 gtcgttatcg gctctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa
     6541 gtgctgccaa ttaccatcca cggtgacgcc gcagtgaccg gacagggcgt ggttcaggaa
     6601 accctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc
     6661 aacaaccagg ttggtttcac cacctctaat ccgctggatg cccgttctac gccgtactgt
     6721 actgatatcg gcaagatggt tcaggcaccg attttccacg ttaacgcgga cgatccggaa
     6781 gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc
     6841 ttcatcgacc tggtatgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca
     6901 acccagccgc tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct
     6961 gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg
     7021 taccgcgatg cgctggatgc tggcgattgc gtggtagcag agtggcgtcc gatgaacatg
     7081 cactctttca cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa
     7141 gttgagatga agcgcctgca ggaactggct aaacgcatca gcacggtgcc ggaagcggtt
     7201 gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag
     7261 aaactgttcg actggggcgg cgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc
     7321 attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg
     7381 gtgatccaca accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg
     7441 cagggcgcgt tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa
     7501 tacggttatg ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac
     7561 ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cctctggcga gcagaaatgg
     7621 ggccggatgt gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag
     7681 cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtg
     7741 tgcgtaccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg
     7801 atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt
     7861 tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag
     7921 cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg
     7981 ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc
     8041 tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat
     8101 tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat
     8161 ttccgagaag tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc
     8221 tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac
     8281 gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg
     8341 acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg
     8401 cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac
     8461 cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt
     8521 ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca
     8581 aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa
     8641 acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca
     8701 gcgccattaa aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc
     8761 tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg
     8821 ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag
     8881 agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca
     8941 tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca
     9001 tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc
     9061 cggaagtgaa cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca
     9121 gcatggcggt ttctacgccg cgcggcctgg tgacaccggt actgcgtgat gtcgataccc
     9181 tcggcatggc agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca
     9241 agctgaccgt tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg
     9301 gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg
     9361 ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc
     9421 tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa
     9481 cgatcaaaga gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc
     9541 ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga
     9601 ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg
     9661 cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag
     9721 aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg
     9781 gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg
     9841 cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg
     9901 ttaaccagat tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg
     9961 ttgtagaccg tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa
    10021 tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga
    10081 ctggcccgat gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac
    10141 tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc
    10201 tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg
    10261 acggcaaact gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc
    10321 gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg
    10381 ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga
    10441 tggacatcgt taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg
    10501 caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag
    10561 ccgttctggt taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca
    10621 tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca
    10681 acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag
    10741 gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt
    10801 aattgataaa aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca
    10861 ctcagaacag gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg
    10921 cggtactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac
    10981 tggcgctacc gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga
    11041 agctattgat gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga
    11101 tatgctgact gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg
    11161 cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa
    11221 accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca
    11281 gaccacggat tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc
    11341 tggctctaac tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat
    11401 cgtaatgatc ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga
    11461 gcatgttacc aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg
    11521 tatgggccac gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc
    11581 tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact
    11641 gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca
    11701 ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt
    11761 gcttatcgcg atgattttcg ttgcgctatc agggtaaatt tatagtcatc ggtattaaaa
    11821 gcgttgcggc tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca
    11881 agaatcggct ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga
    11941 ggaatgattt cctgttcgct acgatcgata accattttct tcacttcttc aataaagtga
    12001 tattttgaat tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag
    12061 gtttcttcca gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca
    12121 cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca
    12181 aatatcagga cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg
    12241 gttaactgaa aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga
    12301 atgctttcga ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg
    12361 ctgacgccaa actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc
    12421 tcgccacgcg caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt
    12481 cccattttta gtatctcatt aatacgaatt tagccattat gcccgataaa ttca
//
LOCUS       DAZFFI010000129        12513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFFI010000129 DAZFFI010000000
VERSION     DAZFFI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.129"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHJ12_004174"
     CDS             <1..222
                     /locus_tag="ACHJ12_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4991883.1"
                     /translation="TSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(262..1476)
                     /gene="ydcO"
                     /locus_tag="ACHJ12_004175"
     CDS             complement(262..1476)
                     /gene="ydcO"
                     /locus_tag="ACHJ12_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4991884.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1529..2065
                     /gene="sutR"
                     /locus_tag="ACHJ12_004176"
     CDS             1529..2065
                     /gene="sutR"
                     /locus_tag="ACHJ12_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4991885.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2138..4099
                     /gene="rlhA"
                     /locus_tag="ACHJ12_004177"
     CDS             2138..4099
                     /gene="rlhA"
                     /locus_tag="ACHJ12_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4991886.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4191..4421)
                     /gene="yncJ"
                     /locus_tag="ACHJ12_004178"
     CDS             complement(4191..4421)
                     /gene="yncJ"
                     /locus_tag="ACHJ12_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4991887.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4643..4797
                     /locus_tag="ACHJ12_004179"
                     /pseudo
     CDS             4643..4797
                     /locus_tag="ACHJ12_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4843..5259
                     /gene="hicB"
                     /locus_tag="ACHJ12_004180"
     CDS             4843..5259
                     /gene="hicB"
                     /locus_tag="ACHJ12_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4991888.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5338..6745
                     /gene="ydcR"
                     /locus_tag="ACHJ12_004181"
                     /pseudo
     CDS             5338..6745
                     /gene="ydcR"
                     /locus_tag="ACHJ12_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6990..8135
                     /gene="ydcS"
                     /locus_tag="ACHJ12_004182"
     CDS             6990..8135
                     /gene="ydcS"
                     /locus_tag="ACHJ12_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4991889.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8153..9166
                     /gene="ydcT"
                     /locus_tag="ACHJ12_004183"
     CDS             8153..9166
                     /gene="ydcT"
                     /locus_tag="ACHJ12_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4991890.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDCVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9167..10108
                     /gene="ydcU"
                     /locus_tag="ACHJ12_004184"
     CDS             9167..10108
                     /gene="ydcU"
                     /locus_tag="ACHJ12_004184"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4991891.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10098..10892
                     /gene="ydcV"
                     /locus_tag="ACHJ12_004185"
     CDS             10098..10892
                     /gene="ydcV"
                     /locus_tag="ACHJ12_004185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4991892.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10914..12338
                     /gene="patD"
                     /locus_tag="ACHJ12_004186"
     CDS             10914..12338
                     /gene="patD"
                     /locus_tag="ACHJ12_004186"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4991893.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARVQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3026 a         3068 c         3358 g         3061 t
ORIGIN      
        1 acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaatcgcct gtcacaaagt
       61 aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaatgg cgctcgtaac
      121 attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc aggccgcccg
      181 ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca gggatcctcg
      241 tccttcaggg cgtggaggat gtcaatatcg gtttctgtca gcgattaaat tcaacaccac
      301 gtaacaaacg cctccggcaa ttaatcccca gaacgcagaa ccaatcccga ccagcgtcaa
      361 tccacttgcc gttaccagaa atgccaccac cgccgcgtcg cgctcacgct cattatgcag
      421 cgcctgatac aaactgccgc taatggtact taacagcgcc agacctgcca gcatctggat
      481 ccaacttacg ggcagggcag ccatcatccc ggtaatagca ctaccaaaca gacctgcgat
      541 caaataaaaa atgcctgcaa cggcagctgc cagccaacgt tgatctttat ccggatgcgc
      601 ttccgggctt tggcaaatag ccgcggtgat tgccgcaata ccgacggaat aaacgccgaa
      661 aggggaaaaa accagtgcca gcaatccagt aaatacaatt aatggcgaaa caggagccga
      721 atacccggct gctttcattg ctgcgatacc cggtgcgttt tgcgatgcca tcgtcaccag
      781 aaaaaggggg agtgcaacgc tcaggctgtg agcaaacgaa aaatcagggg taatataagt
      841 ggggagaacg ggtttaaaga caacatcagt tgtgacaacg tcaccttgcg cgataacgat
      901 cacgatccca ataatcatcg cggcaattac cgcatagcgc ggcgcaacgg ccttggttgc
      961 cagccatacc agcaacatac ttccacacaa cgtaaattga ccgtccagac tggcaaacgc
     1021 ctgtaaacca aagcgtaata aaatcccggc aagcattgcc gccgcaagcg agtgcggaat
     1081 aatgcgcatc agacgagcaa agagtcccgt tatgccgcag aggactatta acgcgttggt
     1141 gacaataaaa acgccgatgg cttcgttaag tgttagtccc tgcaatccgg tgaccaacaa
     1201 agccgcgcca ggcgttgacc atgcggtgag aacaggtacg cgataccata atgtcagagt
     1261 cagcgtactg acgcccattg ccagccccag cgccgtcatc cagccagaga tttgtgcagt
     1321 ggtggctccg gcgacaatcg ctgcttgcca gattattgcc gctgaactgg cgtagccaat
     1381 taataccgcc agaaaccccg ccagtagcgt gggtggagga atagaaaaca gacgcatagt
     1441 cacctcgtgc gttatagcgt acaggcaccg tagcatttgt ccgttataac gcacaagtga
     1501 taaacttccg ttttgccgga ggagtcgcat ggaaaatctc gctcgctttt tatccaccac
     1561 acttaaacaa ctacgccagc agcgcggctg gagtctttcg cgactggcag aggcgacggg
     1621 cgtttctaaa gcaatgttag ggcagattga gcgtaatgag tccagtccga cggtcgcgac
     1681 attatggaaa attgccaccg ggttgaacgt accattttca acatttattt ctccgccgca
     1741 gtccgctacg ccttcagttt atgatccaca acagcaggcg atggtgatca cttcgctgtt
     1801 tccttatgat ccacaactct gcttcgaaca cttctcgatt cagatggcat ctggtgcaat
     1861 aagcgaatcg acgccccatg agaagggggt aatcgaacat gtcgtcgtca ttgatggaca
     1921 acttgatctg tgcgttgatg gggaatggca gacccttaat tgtggggaag gcgttcgatt
     1981 tgctgcagac gtcacgcata tctatcgtaa tggtggggag caaaccgtac attttcattc
     2041 cctcatccat tacccgcgca gttaagcggg aaaactattt cgcaacgtcg cgcttgtggc
     2101 taaaatagcc gccatttttc agctactgga taagaatgtg accgtatctt ctcatcgact
     2161 tgaactgtta agcccggcac gcgatgccgc cattgcccgc gaagctattt tgcacggtgc
     2221 cgatgctgtt tatatcggcg gccctggttt tggtgcccgt cataatgcca gtaatagcct
     2281 gaaagatatt gccgagctgg tgccgtttgc ccatcgttat ggtgcaaaaa ttttcgtcac
     2341 gcttaacacc attttgcatg atgatgagct ggaacccgcg caacggctga ttaccgacct
     2401 ctaccagacc ggtgtcgatg cgctgattgt tcaggatatg gggattctgg aacttgatat
     2461 tccgccgatt gaactgcacg ccagtacgca gtgcgacatt cgtacagtag aaaaagcgaa
     2521 gttcctctct gatgttggct tcacgcagat tgtgctggcg cgagagctga atcttgagca
     2581 gatccgcgcg attcaccagg ccacggacgc gaccattgaa ttctttattc atggggcact
     2641 gtgcgtggcc tattcgggtc agtgctacat ttctcatgcg caaacagggc gtagcgccaa
     2701 ccgtggtgat tgctcgcagg cgtgccgttt gccatacaca ttgaaagacg atcaggggcg
     2761 ggtggtttcc tatgaaaaac atctgctgtc gatgaaagat aacgatcaga ctgccaacct
     2821 cggcgcgctg attgatgctg gcgtacgctc cttcaagatt gaagggcgtt acaaagatat
     2881 gagctacgtg aagaatatca ccgcccatta tcgccagatg cttgatgcca ttattgaaga
     2941 acgtggcgat ctggcgcgcg cttcatcagg tcgtactgaa catttctttg ttccatcgac
     3001 ggaaaagact ttccaccgtg gtagcacaga ttattttgtg aatgcccgta aaggcgatat
     3061 tggcgcgttc gattcgccga aatttatcgg cctgccggta ggcgaagtat tgaaagtggc
     3121 gaaagatcat ctcgatgttg ccgtcaccga gccactggca aatggtgatg gcctgaacgt
     3181 gatgattaaa cgtgaagtcg tcggttttcg tgccaatacg gtcgagaaaa ccggagaaaa
     3241 tcagtaccgc gtctggccca atgaaatgcc agcagatttg cacaaaattc gtccacatca
     3301 cccactaaac cgtaatcttg atcataactg gcagcaggca ctgacaaaaa cctccagcga
     3361 acgtcgggtg gcggtagaca ttgaactggg cggctggcag gaacaactga ttctgaccct
     3421 caccagtgaa gagggtgtca gcatcacgca tacgctggac gggcagttcg acgaagccaa
     3481 taacgccgaa aaagcaatga acaatctgaa ggatggtctg gcaaaactgg ggcaaaccct
     3541 ctattacgcc cgcgatgtgc aaattaattt gccgggggcg ctgtttgtac caaacagtct
     3601 gttaaaccag ttccgccgtg aagctgctga catgctggat gctgcgcgtc ttgccagtta
     3661 ccagcgcggc agccgtaaac cggttgctga tcctgcgccg gtttatccgc aaacgcatct
     3721 gagtttcctc gcgaacgtat acaaccagaa agcgcgtgaa ttttatcatc gctatggtgt
     3781 gcagctgatt gacgcggcgt atgaagcaca tgaagagaag ggcgaagtcc cggtgatgat
     3841 caccaagcat tgtctgcgct ttgcctttaa tctgtgcccg aaacaggcga aaggcaatat
     3901 caaaagctgg aaggcgacgc caatgcaact ggttaacggc gatgaagtat taacgctaaa
     3961 gtttgattgc cgcccatgcg agatgcacgt cattggcaaa atcaaaaatc acatactgaa
     4021 aatgccgtta ccgggaagcg tagtggcatc cgtaagtccg gatgagctgc tgaaaacatt
     4081 gcctaagcga aaagggtaaa acgccagttt tctggttact cacaactcat tgaatctgca
     4141 tgatattgcc tgccgggtaa ggcgtctcgc cgcatccggc aacaaatgac tcaacgttgt
     4201 cccgcttctg gtttgcgcga tttttgccag taaaaatgct cgcgcaaacc ttccgccgac
     4261 tcttccgcca cagcacgcaa ttcatcgctg tccgcttcat gacgcagctg atgatccaca
     4321 ttctttaccc acacaaattc atgtcctttg tgccctgcca tgagttgtcc tgagaacagc
     4381 gcacacgtta ataagacaac cgataacgcc ttcgtaaaca tcctgccacc tttttcttac
     4441 cttttgccgc tatgatgccg atcgtttctt gaggttatta ttcagttttg caaattagcg
     4501 caaagaaatt ctggaatctt ccttcctgat tttgcatggc attctgccgt tgcggcgatt
     4561 tagtgctatt ttcgagcaaa ttacacacgg aggtaaacaa taatgtttac tttgttgata
     4621 tactcagcgg cagggaggcg atgtgaaaca aagcgagttc agacgttggc tcgaatctca
     4681 gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc aggtttcatg ggaggcgcag
     4741 tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac aactcggttt gagttaatcg
     4801 ccaattaaaa aggttaatga catgcgagag acagtcgaaa ttatgcgtta tcccgtcact
     4861 cttacacccg cgccggaagg cggttatatg gtttcttttg tggatatccc tgaagcgttg
     4921 acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa aagatgcttt actgaccgca
     4981 tttgattttt attttgaaga taacgagctt atcccgttac cttcgccatt aaatagtcat
     5041 gatcacttta ttgaagtacc tttgagcgtc gcctctaagg tattgctgtt aaatgctttt
     5101 ttacagtcag aaatcactca gcaagagtta gccaggcgaa ttggcaaacc taaacaggag
     5161 attactcgcc tatttaactt gcaccatgcg acaaaaatcg acgccgtcca gcttgcggca
     5221 aaggcgcttg gcaaagagtt atcgctggtg atggtttaat tacagttaac gaaaagttgt
     5281 catttttaac aactgatata gactgccgaa tcatctgcac ataattacga ttcgataatg
     5341 aaaaaatacc agcagcttgc agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa
     5401 cccggcgatc gtttgccttc gttgcgtgac caggtggcgc tttcaggcat gagctttatg
     5461 actgtcagcc atgcctatca gttgctcgaa agtcagggat atattatcgc acgaccgcag
     5521 tcgggttatt acgttgcgcc acaggcaata aaaatgccga aagcgccagt cattccagtc
     5581 actcgagatg aagcagtcga tatcaacact tatatttttg atatgttgca ggccagtcgc
     5641 gatccgtcgg tcgttccgtt tgcctcggcc tttcccgacc cgcgactttt ccccctccaa
     5701 caactaaacc gctcgctggc gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa
     5761 aacttaccgc caggaaacgc agaactgcgt caggctattg ctcgtcgcta tgccttacag
     5821 ggcatcacca tttctcctga tgaaattgtc attactgccg gggcgttaga ggcattaaac
     5881 ctcagtttgc aagcggtaac tgaaccgggc gattgggtga tagtagagaa tccttgtttc
     5941 tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg cgttatcggt ggcgacggat
     6001 gttaaagaag ggatcgatct tcaggcgctg gaactggcgt tgcaggatta tccggtgaaa
     6061 gcgtgctggc tgatgactaa tagccagaac ccactcggat ttaccttaac gccgcaaaaa
     6121 aaaagcacaa ctggtggcgt tactcaatca gtacaacgta acgctgattg aagatgacgt
     6181 ttacagcgaa ctttattttg gacgggaaaa accgctgcct gcgaaagcgt gggatcgcca
     6241 cgatggcgtt ttgcattgct cttcgttttc gaaatgtctg gtgcctggtt ttcgtattgg
     6301 ttgggtcgcc gccggaaaac atgcacgtaa aattcaacgc ttgcagttga tgagtacgct
     6361 ttccaccagc tcaccgatgc aacttgcgct ggtggattac ctttccacgc gccgatacga
     6421 cgcccatctt cgtcgcctgc gtcgccagct tgcggaacgt aaacaacgtg cctggcaggc
     6481 actgctgcgt tatctgcctg cggaagttaa aattcatcat agtgacagcg gttattttct
     6541 ctggctggag ctccccgagc cgttagatgc cggcgaatta agcctggcgg cactgacgca
     6601 tcatatcagt attgcgccgg gtaaaatgtt ttctaccggt gaaaactggt cacgtttttt
     6661 tcgttttaat accgcgtggc agtggggaga acgtgaagaa caggcggtaa aacaattagg
     6721 caaacttatt caagaacggc tgtaatagcg tttaatttaa ttcctcttag attgggtaat
     6781 atgaatttcg aatagcagtc atatttccta accccttgtc tatactccag aagataacct
     6841 tacagacggc ataatgcgcg gtagctcaca acctgattaa attttctcag gggcgaaggt
     6901 gtgcctgcaa gccgccgtct atggttaaac aaggagatat ttttacggca cggcggctga
     6961 acaattaatt acgacaggag taagacctta tgagcaagac atttgcccgc agcagcctgt
     7021 gtgcgctcac catgacaata atgaccgctc acgccgccga accacctacc aatttagata
     7081 aaccggaagg gcgactggat attatcgcct ggccgggata catcgaacgc ggacaaactg
     7141 ataaacaata cgactgggta acgcaatttg aaaaagagac aggctgcgcg gtgaatgtga
     7201 aaaccgccgc gacttccgat gaaatggtca gtctgatgac caaagggggt tacgatctgg
     7261 ttacggcatc cggcgatgcc tcgctgcgtt tgattatggg caaacgcgtg cagccgatta
     7321 ataccgcctt gattcccaac tggaaaacgc tcgatccgcg cgtggttaaa ggcgactggt
     7381 ttaatgttgg cggcaaagtt tacggcacac cttaccaatg ggggccgaac ctgctgatgt
     7441 acaacactaa aaccttcccg acgccgccgg atagctggca agtggttttt gttgagcaaa
     7501 atctgccgga cggcaagagc aataaaggcc gcgttcaggc ttatgatggc cctatctaca
     7561 ttgcggacgc tgcgttgttc gttaaagcca ctcagccgca gttgggcatc agcgatccgt
     7621 atcaactaac cgaagaacag taccaggcgg tgctgaaagt gctgcgcgct caacacagtt
     7681 tgatccatcg ctactggcat gacactaccg tgcaaatgag cgatttcaaa aacgagggtg
     7741 tggttgcttc cagtgcctgg ccctatcagg ccaacgccct gaaagccgaa ggccagcctg
     7801 ttgctaccgt tttcccgaag gagggtgtta ccggttgggc tgataccacc atgctgcata
     7861 gcgaagcgaa acatccggtt tgcgcctaca aatggatgaa ctggtcatta acgccaaaag
     7921 tgcagggcga tgtggcggcc tggtttggct cgttaccggt agtgccggaa gggtgtaaag
     7981 ccagtccgtt attaggcgaa aaaggttgtg aaaccaacgg ttttaactat ttcgacaaaa
     8041 tcgccttctg gaaaacgcct atagcagaag ggggcaagtt tgttccctac agtcgctgga
     8101 cgcaggatta cattgccatt atgggcggtc gctaacttcg ctggggtact ttatgacgta
     8161 cgcagtggag tttgacaacg tctcgcggtt gtacggtgac gtgcgcgcag tagattgcgt
     8221 cagtattgcg ataaaagatg gtgagttctt ctctatgctg gggccgtccg gctccggcaa
     8281 aaccacctgc ctgcgcctga ttgctggctt cgaacagctt tccggcgggg ctatctctat
     8341 ctttggtaaa cccgccagca atctgccacc gtgggagcgg gacgtgaata ctgtctttca
     8401 ggactacgcg ctatttccgc atatgtcgat tcttgacaat gtcgcctatg ggctgatggt
     8461 caaaggcgtg aataaaaagc agcggcacgc aatggcgcaa gaggcgctgg agaaagtggc
     8521 gttggggttt gtacatcaac gtaaaccgtc acaactttct ggtggtcagc gccagcgggt
     8581 tgctatcgcc agagcattgg tgaatgaacc gcgcgtattg ctgttggatg aaccgctcgg
     8641 cgcactggat ctcaaattgc gtgagcagat gcagctggaa ctgaaaaaac tgcaacagtc
     8701 tctcggtatc acttttatct tcgttaccca cgatcagggc gaagcgttat cgatgtccga
     8761 tcgtgtggcg gttttcaata atggacgcat tgagcaggtc gattccccgc gcgatctcta
     8821 tatgcgcccg cgcacgccgt ttgttgccgg attcgtcggg acatccaacg tttttgatgg
     8881 actgatggca gagaaacttt gtggcatgac gggaagcttc gccctgcgac cggaacatat
     8941 ccgcctcaac acccctggtg aactgcaggc caatggcacg atccaggcgg tgcaatatca
     9001 gggcgcggca actcgttttg aactgaaatt gaacggcggt gaaaaactgc ttgtgagtca
     9061 ggccaatatg acaggcgaag aactgcctgc cacgctcacg cccggacaac aggtgatggt
     9121 ttcctggtcg cgtgatgtga tggtgccgct ggttgaggag aggtgaatgg cgatgaatgt
     9181 attgcaatca ccttcacgtc caggtctggg taaggtgtcc ggtttcttct ggcgtaatcc
     9241 ggggctgggg ctgtttttac tgctgcttgg cccgctaatg tggtttggca ttgtctattt
     9301 cggctcgctg ctgacactgt tatggcaggg attttatact tttgacgatt tcaccatgtc
     9361 ggtaacgccg gaactgacgc tggcgaatat ccgtgcgctg tttaatccgg ctaattacga
     9421 catcattctc cgcacgctga ctatggctgt ggcagtcact atcgccagcg ccattctggc
     9481 ttttccaatg gcgtggtata tggcgcgcta taccagcggg aaaatgaaag ctttttttta
     9541 tattgcggta atgttgccga tgtgggcgag ctacattgtt aaagcctatg cctggacgtt
     9601 attgctggca aaagatggcg tggctcagtg gtttttacaa catcttgggc tggaaccact
     9661 gctgactgcg ttccttacat tacctgcggt gggcggaaat acgctgtcaa cttccgggct
     9721 ggggcgcttt ctggtgtttc tctatatctg gttgccgttc atgatcctgc ccgttcaggc
     9781 ggcgcttgag cgtttgccgc cgtcattgtt gcaggcgtcg gctgatctcg gcgcacgtcc
     9841 acgacaaacc tttcgctatg tggtgctgcc gctggcaatc ccgggtattg ccgctggctc
     9901 tatctttacc ttctcactca cactgggcga ttttatcgtc ccgcagctgg ttggtcctcc
     9961 aggatatttt atcggcaata tggtttattc ccagcagggg gcgattggca atatgccgat
    10021 ggcggcggca ttcaccctgg tgccgattgt tctcatcgca ctgtacctgg cgttcgtgaa
    10081 acgtctggga gcgttcgatg cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct
    10141 ggggcggcgt ggttttccta cattttccca tcctgattat cgccgcttat gcgtttaaca
    10201 ctgaagatgc ggcgtttagt tttccaccgc agggcctgac gctgcgctgg tttagcgtgg
    10261 cagcacagcg tagtgatatt cttgatgccg tgacactgtc acttaaagtg gcggcgctgg
    10321 cgacattaat ggcgctggtg ttagggacgc tggcagctgc cgcgctgtgg cgacgagact
    10381 ttttcggcaa aaacgccatt tcgctgttac tgctgctgcc cattgcgctg ccgggcattg
    10441 tcactggtct ggcgctatta accgctttta aaaccatcaa tctggagccg ggatttttca
    10501 ccatcgtggt cggtcatgcg actttttgcg tagtggtggt gtttaacaat gtcatcgccc
    10561 gttttcgccg cacctcgtgg agtctggttg aggcgtcaat ggatcttggg gccaatggct
    10621 ggcaaacctt ccgctacgta gtgttgccga atctcagttc ggcgctactg gcaggaggaa
    10681 tgctggcgtt tgccttgtcg ttcgatgaaa tcatcgttac gacttttacg gcaggtcatg
    10741 aacgaacgtt accgttgtgg ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa
    10801 ctaacgtggt ggcactgctg gttatgttgg taacaacctt gccgatcctg ggggcctggt
    10861 ggctaacccg cgaaggcgac aatggtcaat aaccactgat acaggaatat gctatgcaac
    10921 ataagttact gattaacgga gaactggtta gcggcgaagg ggaaaaacag cctgtctata
    10981 atccggcaac gggggacgtt ttactggaga ttgccgaggc atccgcagag caggtcgatg
    11041 ctgctgtgcg cgcggcagat gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc
    11101 gtgcggaatg tctgctgaaa ctggctgatg ttatcgaaga aaatggtcag gtttttgccg
    11161 aactggagtc ccgtaattgt ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg
    11221 cgattgtcga tgtttttcgc tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg
    11281 caggtgaata tcttgaaggt catacttcga tgatccgtcg cgatccgttg ggggtcgtgg
    11341 cttctatcgc accgtggaat tatccgctga tgatggcagc gtggaaactg gctccggcgc
    11401 tggcggcagg gaactgcgta gtgcttaaac catcagaaat taccccgctg accgcgttga
    11461 agttggcaga gctggcgaaa gatatcttcc cagcaggcgt gattaacgta ctgtttggca
    11521 gaggcaaaac ggtgggtgat ccgctgaccg gtcatcccaa agtgcggatg gtgtcgctga
    11581 cgggctctat cgccaccggc gagcacatca tcagccatac cgcgtcgtcc attaagcgta
    11641 ctcatatgga acttggtggc aaagcgccag tgattgtttt tgatgatgcg gatattgaag
    11701 cagtggtcga aggtgtacgt acatttggct attacaatgc tggacaggat tgtactgcgg
    11761 cttgtcggat ctacgcgcaa aaaggcattt acgatacgct ggtggaaaaa ctgggtgctg
    11821 cggtggcaac gttaaaatct ggtgcgccgg atgacgagtc tacggagctt ggacctttaa
    11881 gctcgctggc gcatctcgaa cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc
    11941 acatcaaagt gatcactggc ggtgaaaagc gcaagggtaa tggctattac tatgcgccga
    12001 cgctgctggc tggcgcatta caggacgatg ccatcgtgca aaaagaggta tttggtccag
    12061 tagtgagtgt tacgcccttc gacaacgaag aacaggtggt gaactgggcg aatgacagcc
    12121 agtacggact tgcatcttcg gtatggacga aagatgtggg cagggcgcat cgcgtcagcg
    12181 cacgggtgca atatggttgt acctgggtca atacccattt catgctggta agtgaaatgc
    12241 cgcacggtgg gcagaaactt tctggttacg gcaaggatat gtcactttat gggctggagg
    12301 attacaccgt cgtccgccac gtcatggtta aacattaagt tagatgttag tggctgttat
    12361 aggacggcca ctatcgtttt aggttctgag tttaatggcg gttaagtatt agaatgatta
    12421 cattaataac tagcgtaaaa attaggctaa atgtttgatg gcgatttttc atcctgtcac
    12481 agttgattag cataaggctt tagagtatcg tta
//
LOCUS       DAZFFI010000130        12449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFFI010000130 DAZFFI010000000
VERSION     DAZFFI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.130"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..374)
                     /locus_tag="ACHJ12_004187"
     CDS             complement(<1..374)
                     /locus_tag="ACHJ12_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO4991894.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATIL"
     gene            complement(1195..1407)
                     /locus_tag="ACHJ12_004188"
     CDS             complement(1195..1407)
                     /locus_tag="ACHJ12_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO4991895.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(1483..2100)
                     /locus_tag="ACHJ12_004189"
     CDS             complement(1483..2100)
                     /locus_tag="ACHJ12_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4991896.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2367..3866
                     /gene="ansP"
                     /locus_tag="ACHJ12_004190"
     CDS             2367..3866
                     /gene="ansP"
                     /locus_tag="ACHJ12_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4991897.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3981..5042)
                     /locus_tag="ACHJ12_004191"
     CDS             complement(3981..5042)
                     /locus_tag="ACHJ12_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4991898.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5284..7386
                     /gene="pqqU"
                     /locus_tag="ACHJ12_004192"
     CDS             5284..7386
                     /gene="pqqU"
                     /locus_tag="ACHJ12_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4991899.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7422..8087)
                     /gene="mcbR"
                     /locus_tag="ACHJ12_004193"
     CDS             complement(7422..8087)
                     /gene="mcbR"
                     /locus_tag="ACHJ12_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4991900.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(8285..9322)
                     /gene="curA"
                     /locus_tag="ACHJ12_004194"
     CDS             complement(8285..9322)
                     /gene="curA"
                     /locus_tag="ACHJ12_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4991901.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            9503..10021
                     /gene="mddA"
                     /locus_tag="ACHJ12_004195"
     CDS             9503..10021
                     /gene="mddA"
                     /locus_tag="ACHJ12_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4991902.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            10018..10467
                     /locus_tag="ACHJ12_004196"
     CDS             10018..10467
                     /locus_tag="ACHJ12_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4991903.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(10468..10701)
                     /gene="ydcY"
                     /locus_tag="ACHJ12_004197"
     CDS             complement(10468..10701)
                     /gene="ydcY"
                     /locus_tag="ACHJ12_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4991904.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(10787..10960)
                     /gene="ortT"
                     /locus_tag="ACHJ12_004198"
     CDS             complement(10787..10960)
                     /gene="ortT"
                     /locus_tag="ACHJ12_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4991905.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            11155..11250
                     /gene="yncL"
                     /locus_tag="ACHJ12_004199"
     CDS             11155..11250
                     /gene="yncL"
                     /locus_tag="ACHJ12_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4991906.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(11652..>12449)
                     /locus_tag="ACHJ12_004200"
     CDS             complement(11652..>12449)
                     /locus_tag="ACHJ12_004200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO4991907.1"
                     /translation="LYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNL
                     FLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVK
                     IMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDV
                     VLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNY
                     NYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         3188 a         2954 c         2868 g         3439 t
ORIGIN      
        1 ttcaggatcg tagcgaggat gctttcgatg tcctcattct ggaagatacg gaagttctgg
       61 cgcagccctg tgcgccacag cggcgggcac accttcatgc tgtacagcat ctggtttttg
      121 tcgttctccc ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg
      181 ccctgccata ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggaaaga
      241 aactgctgac tgaccagaga aaggtcgagg gaaaaaagtg aagagagtga ctgggtgaga
      301 tgaaaggaaa ccaccgcaaa cgcgtccggt ggcaggccgt ccacttcaag tgtgaaacgt
      361 aatccggttg acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta
      421 caacatgtcg ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga
      481 aaaatgtcat ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct
      541 gtttagtcaa caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag
      601 gctcgtacat tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata
      661 atgtcttttt attcacaaca ataaataatt caataacaat cattgaaatg aatgatgatt
      721 tgtattagga atatacgcta ccgccccacc ctgataacat gatctgcaca ttgccatctt
      781 aacgtcgtta aacactggct taatagtgga tatttcaaat taggaattta ttaaattcac
      841 ctgatgaata tcataagcaa atagcataga aaataatgac aattcccccg aggggaccat
      901 ttccaaagca ctgcaccacc accaggtgcc cggatgatct tgcttcagtg gtaagagtac
      961 ctgattcatt gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca
     1021 cgtgataatc tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct
     1081 tgaataaatc aacatgttta tttatcaatt gctgttttag ctctgactca tcaagctctc
     1141 catatttgtc ctacagataa ttgcgaataa atacagagaa cgccatttat ccttttagca
     1201 tacctgccgg atatttttat ttgtattaat attaattgca tctccaactc cgagcatatc
     1261 catcattaaa aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac
     1321 aatgtgaata gttagtttct tttcagagga aatgaaatag acaagctgaa tgaaggggag
     1381 agtgatataa atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata
     1441 aagttactga ataataaata acataatcct ctggtatccc actcaaataa tttcattcct
     1501 ttttaatact tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt
     1561 cgtggcatta tcctgaaacc attcatgccc aggcccccat gtgcgcatag tgcaaagata
     1621 acagtccacc agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt
     1681 cagccagata taaagcgatt tacgatattc aattacattc ttctttaact gttcaggtgc
     1741 gtcaggagcc caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac
     1801 cagccagacc aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag
     1861 gtccgggcga cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc
     1921 attttccagc gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg
     1981 tgaagttccc tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag
     2041 tgttagcatc agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat
     2101 atgcccctca caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa
     2161 ggatttgtca ttttttcgtc agttgtatta atccccttca ggattgtaag gggatgtttt
     2221 gcatcgttta tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata
     2281 atggtgataa ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt
     2341 tttcattaaa gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac
     2401 acgccgcgaa acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca
     2461 atcgccaggt acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg
     2521 caggagcccg actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct
     2581 tgttttcgtt ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg
     2641 gcagttttgt ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct
     2701 ggatgtactt catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt
     2761 atatgcatta ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac
     2821 ttaccatcgt tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct
     2881 ggtttgcgct tattaaagtg ctcgccattg tgatcttttt ggtcgtgggt acagtgttcc
     2941 tcggtagtgg tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg
     3001 gcggcttctt cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg
     3061 cttttgcctc cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca
     3121 tggtgcctaa agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg
     3181 tggtgttgct ggttatgcta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg
     3241 tgacgttttt ctctaaactg ggtgtgccat atatcggcag cattatgaac attgtggtgc
     3301 tgaccgctgc cctctccagc ctgaactcag gtctgtactg caccggacgt attctgcgct
     3361 caatggcgat gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc
     3421 cgtatgccgg gattctagcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc
     3481 tggtgccgtc gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt
     3541 catgggcgtt tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag
     3601 cagcggatgt cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt
     3661 ttttactgag tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg
     3721 cggcgctgcc gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg
     3781 ttgctgaaat tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg
     3841 tgtttaagcc tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt
     3901 tagattactg acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt
     3961 tttcaccctc gccacatcta ttacagcgca atacgaatca catcgtccgg ctgggtagct
     4021 tcctgctgtt tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac
     4081 agcgccaggc tgtttggatg agtcggcgta tcgaacgttt tcaccacttt atagcttttc
     4141 gcgtcaatca cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct
     4201 gggttaaaca gcacagccag tgattctggt gccgcaacct tcgccagaat attgccatta
     4261 cgggtatcga ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgcctg
     4321 gcggtatcaa ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta
     4381 cggctgagga ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg
     4441 gtgtaaagac gtttgccttt gctatccagc gccagaccgg tactcatttt accggtgttc
     4501 tggatggcgg ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta
     4561 ccaataccac tgatgtaaac ggtgttcgtg gcatcatcag ctaccagctc acgcggttgc
     4621 agcgggcgca cctcttccgt gcgcttacga tcatccagca ccagacggcc tttcacctcg
     4681 cccgttttgg catctatcgc cgtgaccgcg ctgtttacgg tgttaccaaa ccacaacgtc
     4741 tgagtcgtgt tattgatggt ggcaccaaac ggcttgagat cgttatggat cgcctgcgtc
     4801 acttccagag tgaccggatc aagacgataa accacgccac ctttatccag tttgcggctt
     4861 tgcgaagtgg cgagccacag tgcgttttct tgctggctat aagccatttc gtaggcacct
     4921 ttacctaccg ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca
     4981 agcaatgaac ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc
     5041 atgacgactc cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta
     5101 atcatcgtta cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag
     5161 catttcttgc cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt
     5221 ttccataaat ttacaatttc attaccataa cggcgttttc ccattcttaa cctggctttt
     5281 ttcatgaaga ttttttccgt ccgacagacc gttttgcccg cactgcttgt cctttccccc
     5341 gttgtttttg ccgctgatga acagactatg attgtcagtg ccgcaccgca ggtggtttca
     5401 gaactggata ccccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca
     5461 ccgcgcatta acttgtccga atcactgacc ggcgtgcctg gtttgcaggt acaaaaccgg
     5521 cagaactatg cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac
     5581 ggtattcgcg gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa
     5641 gggcaaacat ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc
     5701 ttctctgccc tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga
     5761 caacagccac caaccattga agccagtagt tactacggca gttttggcag ctggcgctat
     5821 gggctgaaag caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc
     5881 gtctcaacca cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat
     5941 ttagctaatg ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc
     6001 aatagtgtgg atatcaaagc agatgaccca ggtgggctaa ccaaagcaga atggaaggct
     6061 aatccacaac aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact
     6121 caggctgggt tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg
     6181 tatgccggag agcgagaaac gacccagtac cagtcaatcc cgatggcccc ccaacttaac
     6241 ccgtcacatg cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc
     6301 tggacacacc gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa
     6361 aacatgagtg aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag
     6421 tacgggcaaa aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc
     6481 tatttacaga cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac
     6541 agctccgtgt ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc
     6601 ggtgatgcca gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat
     6661 gcctggaata tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg
     6721 tcttatcgtg ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat
     6781 acaattgaga tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt
     6841 cagaccgaca ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac
     6901 aaaaatgccg gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca
     6961 ggagattttc gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt
     7021 tgcaatgaac aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt
     7081 gcgtcgatag gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg
     7141 ggcgatatta tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc
     7201 ttattcaccg ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc
     7261 gataatttat tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga
     7321 tattacgaac cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt
     7381 gagtaattac cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg
     7441 tataaaatag caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg
     7501 tcttttgctt ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc
     7561 gctggattaa tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc
     7621 tcaatcatct cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc
     7681 ctgtttacat taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg
     7741 agcaattcct ggagttccgc caggtcttgc ggggtgaggt tttcaacagc cagagcaact
     7801 gccattaatt ccagttcgta gcggatccga ttgatttcgt ctaattgacg tttcctcact
     7861 tccggaactg taaatgcttg tgcaggtgcg acagaaagcg cattcaccga aaccagacgt
     7921 aataatgctt cacgcacagg tgtaatactc atacctaatt gctccgccag atttttagta
     7981 atcaggcgtg cgccaggttt taatgcgcca atactaagct gatgtttcag gtcgttctca
     8041 acctgcagag tcaaactgac atgtttcatt tttcccgttc caggcatttt aattccttcg
     8101 cgtgttttct gtccaaccct gcctgcatgg cagaaggagt tcacctggtg tattaaagtg
     8161 aataatcata aaccactcac ctttgttgtt attagcagca gaacaatgac gtggttagtt
     8221 aattaaagct gaaaataagc tttttgcctg gatttttatg gcggcttacg ccgccgttga
     8281 aactttaatc atcacccgcg acgcggatca ccactttgcc gaagttttta cccttcagca
     8341 ggccgataaa cgtctgtggc gcattctcta aaccatcagt aatatcttcg cggtagtgga
     8401 ttttatcctc tttcacccat tgccccatct ccttctgaaa ctcatggatg cggtgaccat
     8461 aatcctgagc gataataaac ccttgcaagc gaatacgttt tttcagcacc gttgccatca
     8521 acaggggtaa acgatccgga ccgggtggta gctctgtagc gttatagctg ctcactaagc
     8581 cacaaacggg aatgcgcgca gatgtattaa gtaatggtag cactgcatca aataccttac
     8641 cgcccacgtt ttcataatag atatcaatac cttttgggca cgctttcacc agttgttcgg
     8701 caaaatcatc cgcgtggtga tcaagacaaa catcgaagcc cagcacctcg atagcatggc
     8761 ggcatttttc cgcgccaccg gcaaccccca ccaccctgca acctttaagt ttgccgattt
     8821 gccccaccgt tgctcccaca ggacctgtcg ccgcagctac caccaacgtt tcgccctctt
     8881 taggctgacc gatatccagc agccccatat aagcggtaaa gcctggcatc cccagcacac
     8941 ccagcgacca cgatggattt tgcggatgat cgccaagttt caccagatca tcaccactgg
     9001 atatgtcata gtcctgccat ccactgtagc ccagcaccca gtcgccaggc tgataatcag
     9061 gatgattcga ctccacaaca cggctcaccg tgccgccgac catcacgccg ccaatatcaa
     9121 caggcggtga ataagatggc tcatcgctca tacgtccacg catatacggg tccagggaca
     9181 aataaactgt gcgcagtaac acctgtcctt cacccggtgt ggcgacatca tcttcttcaa
     9241 gacggaaatt ctccggaacc ggtgcgccat gtggacgcga ggccagaacc caacgtcgat
     9301 tacgctgctt ttgttgcccc ataatgctct cctgtgcttt tggcatgaat ccatcaatag
     9361 tagcaggctc acgggattgg ttttgtttgc agccgtcaca tcccctctgc aacacgcaat
     9421 aatttcatcg aggaatccgt tgcttcgggc tgttttcgat agtattgata acatcatcat
     9481 cattatttta tcgaggccag acatgtccat ccgttttgcc cgcaaagccg attgtgctgc
     9541 cattgcggaa atttataacc acgccgtgtt gtatacggcg gctatctgga atgaccaaac
     9601 ggttgatgca gataaccgca ttgcctggtt tgaagcgcgg accatagcag gttatccggt
     9661 gctggtgagt gaagaagatg gcgtagtgac gggatatgcc tcgtttggcg actggcgtag
     9721 tttcgatggt tttcgccata ccgtggaaca ttcggtttat gtccatcccg atcatcaggg
     9781 caaaggtctg gggcgtaaat tgttaagccg attgattgat gaagcgcggg attgcgggaa
     9841 gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcc tcgctgcatc tgcaccagtc
     9901 gctgggattt gtcgtcaccg cacaaatgcc gcaggtgggc actaaatttg ggcgctggct
     9961 ggatctgaca tttatgcagt tgcaactcga cgaacgcact gaaccggacg cgattggatg
    10021 aatcagtcgc tcacccttgc ttttttgatt gccgccggaa ttggtctggt agtccagaac
    10081 acgctgatgg tgcgcatcac ccagacctcc tctaccattc tcatcgccat gttgctgaac
    10141 tcactggtgg ggattgtgct ctttgtttct attttatggt ttaaacaagg catggcgggg
    10201 tttggcgaac tggtgtccag cgtgcgctgg tggacactta tacccggctt gctgggatcg
    10261 ttttttgtct tcgccagtat cagcggatat cagaatgtgg gagcggcgac aactattgca
    10321 gtactggtcg ccagccagct aattggcggt ctgatgctgg atatcttccg cagtcatggc
    10381 gtaccactac gggcgttgtt cggcccaatt tgtggcgcaa ttttacttgt ggttggcgcc
    10441 tggttggtag ccagacgctc attttaatca gaggatggtg ccgcctttgg ttaactgttc
    10501 gtggcgcgct tccatatctt ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa
    10561 acgctgctgc tgggtgtagc gatcttcccg acttaactcc gcgtcatcgc tcagggcgat
    10621 taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat
    10681 gacttcatcc agatgcgaca tactttctcc ttaaccactt gtgaaattta tcgattcatc
    10741 caaatgatat aagtctgcgt ggtgccgtca agccagtgag taagatttaa aacagtgaaa
    10801 acaacagcgc caccgggaaa ctcatcggcc atgttgcccc caccagaaag gcgcttaaga
    10861 aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg attgctgcca
    10921 taaccgcata aaaaacaagc atatgttgat agagagacat gtacgggcgt ccagccagat
    10981 gaataaaacg cgcgcaaata tgcgtcattt tttgacgtac atcaagtttt ttcaccagtt
    11041 tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc ttcagaatga gtattattgc
    11101 ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc aaaaatgaat
    11161 gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt gtttactctt
    11221 atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag cacgtactgt
    11281 cagagaaatc aactctctga cagtacgcaa gatcttctca tactctccag gtttacaaca
    11341 ttatccttaa tatttacttc tttttgctgg catctacatt gaaatgagta gaaaacatcc
    11401 ccctactgtc gtctatacaa acagcttact gattcaaacg gaatcttgtt ttcacatttt
    11461 tctatcatga atactttata cgcgcgggag tctataaatg ctttatactt tcaaggaata
    11521 acgtaaatat aaaaagatgg cgtaaagtat atgcatggca ggatgtttgc aacctccttg
    11581 atgacgctgc cccattgaaa ttgcaaaccc cgccctggtt atcttgccca tttatcatgc
    11641 aaaacagata attataggcc tctgaatacc tcagaaagat tgaatttttt atagtccctc
    11701 tcttgaatat ttccatctga tatcaatcga ttcatctctt caacaaaata atttatatca
    11761 tcacacttgt tattatccgg aagatctgag tagttataat tccaccattt tattctcaac
    11821 agttcatcaa taacatttga atcaaaccta aatctgatta tttttgcagg aactcctgca
    11881 actatggcat atgggggcac atctttggta acaacggaat tggcagcaat aacagcacca
    11941 tcacctattg cgatgccacc tttcaataca acatcatttc ctatccagac atcatttcct
    12001 ataatcggat tttcaggttt ggtaatgaat ggtttcaatg tccagctacg ttcaaattca
    12061 ctcatagcaa acttatcaaa ttcaccgtta taagtcatca tgtgagtagt aaaacgattc
    12121 aaaggatgat ttccacccat tatttttaca tttgatgcaa tactacaaaa tcgacctata
    12181 cgtatattcc ctgaaaaatg gcaggtcgga aatgaaaaag cgccgacact ggagaaattt
    12241 ttcacaggca ttgttgaata ttcttcagca ataacattat cttcaattgt tatcttgtca
    12301 ccttttgaga aaagaaataa attcctttct ttatacgact tcttcccctt atgatttaaa
    12361 aagattttat tatccataca aaactcaaag tgttttttcg tccatctgaa ggtaagggtt
    12421 ttatttttat cttccgtgaa tttgtacaa
//
LOCUS       DAZFFI010000131        12368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFFI010000131 DAZFFI010000000
VERSION     DAZFFI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.131"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            264..1439
                     /locus_tag="ACHJ12_004201"
     CDS             264..1439
                     /locus_tag="ACHJ12_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991908.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWL
                     EKEPRASYFFLFRLYTSIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSVVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGM
                     PPEHLRFATGFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIIN
                     SVFSEWASINNDYPSPFSWVDSRDSGQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLKSNPRRNRAKFTISPFPALRVKQHKAVLLDELKSAREQQKR
                     RDERADGSVPLKLSGKIHKQLESIARSRGVPPKKMLNEMIEQAHRDFVANEQHKTRS"
     gene            complement(2298..3032)
                     /locus_tag="ACHJ12_004202"
     CDS             complement(2298..3032)
                     /locus_tag="ACHJ12_004202"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4991909.1"
                     /translation="MPVLDFKEIPEAHKATGLQDTFELFSRDFLAFLGYKIISDPDRG
                     ADGGVDIIAEEKRTGVGGETIIRWLVSCKHKAHSGNSVNPGDDANITDRVIANNCQGF
                     IGFYSTLASAGLSGILDGLKGRIEFQVFDKEKIERELLHSSRGLEIAERYFPKSLSEW
                     KTENPKPARIFSDKPSLKCKVCKKELLSQDDKGIITIWQRIRTDYEKEPEYNEHIFST
                     CRGNCDQALRQYVRKQTMALLMAGKI"
     gene            complement(3208..3810)
                     /locus_tag="ACHJ12_004203"
                     /pseudo
     CDS             complement(3208..3810)
                     /locus_tag="ACHJ12_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4042..4986
                     /locus_tag="ACHJ12_004204"
     CDS             4042..4986
                     /locus_tag="ACHJ12_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO4991910.1"
                     /translation="MDLIMEWRFLGSLSEARKSGCSGVYLIVHKGLFSRVVYVGVSCN
                     VGRRITEHYDGYLRGNRTIYDAGHDEDVYRFMSAYKIHNHTKYYQALANDYKIWASTT
                     MYSDLPKNMLAKSQTFDTDWQSIALEKYIPQLVVWALPMAKYCYSNASRIESVIQSKL
                     IKSFDLRGFFNIKQLSILGKIEYPYMEKVKVFIINTPDLDPASQLIFSNLYNKKTDNN
                     FCKEFRSQFKSEIFQRESETQRKRTIREHKVSLYENYGKPWTLKEMEKLRVMLVDFDL
                     SPTEISEYLGREPRSISKKISENDKVTNYKWRESVVWL"
     gene            complement(5159..5917)
                     /locus_tag="ACHJ12_004205"
     CDS             complement(5159..5917)
                     /locus_tag="ACHJ12_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991911.1"
                     /translation="MKTLSGKEFRKLFNRYETGFYLSEFEYLKDFDPLFPKCVGKELK
                     LGYKRKVDVWCEEDAALYAEFIATKERLKRGRLILKINQVWSRVYPAYIYAIEERINV
                     RAKGRESFKYETVQEKQCKLSIMLDGLKDLECEIAMISSEFEIEKHSMHYFYMIFDIE
                     RYINDCSYFVFSAAKVVKGVIQCIGYIDKNNKASCDEFYSGSYECLDDDQETLNYLNE
                     SCAEGIDADKDLIRKNELLALSLKELYQLLVSKK"
     gene            6425..7297
                     /locus_tag="ACHJ12_004206"
     CDS             6425..7297
                     /locus_tag="ACHJ12_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4991912.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRAMSVDEYFWRHILHQGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            7669..10515
                     /locus_tag="ACHJ12_004207"
     CDS             7669..10515
                     /locus_tag="ACHJ12_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO4991913.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNDGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDILESHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSDSSAEGYNGQATLNVTF"
     gene            10586..10819
                     /locus_tag="ACHJ12_004208"
     CDS             10586..10819
                     /locus_tag="ACHJ12_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4991914.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            10910..11728
                     /locus_tag="ACHJ12_004209"
     CDS             10910..11728
                     /locus_tag="ACHJ12_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4991915.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFAEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHTLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPALQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESMR"
     gene            11818..12303
                     /locus_tag="ACHJ12_004210"
     CDS             11818..12303
                     /locus_tag="ACHJ12_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4991916.1"
                     /translation="MTTVSHNFTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT         3211 a         3032 c         3000 g         3125 t
ORIGIN      
        1 gatgtatatc cggcacacta tgaccggaga atatcactca agcttaaaaa gataataaat
       61 acagtgagtt aaatacacag agaaaacgta atatcagagg gaacatctta caacagagac
      121 tgaatttccg gtttttcata accggcgcat tccggaaagt gcaggcggcc cggaggtctt
      181 ctgacgccag tacctgatag attgttctgt acattctgta catcatgtac ggaggttttt
      241 gtttgctttc ttaaaatctg caaatgtggg aagagcaggt gaggtgtatt gtgggaaagg
      301 aactggatga tatctgcacc agctgtccgt atattgatgc tgtaaaacgg cacaaacaac
      361 agctcggtgc catagaggaa tatacacagt ggttagaaaa agaaccacgg gcatcttatt
      421 tttttctgtt ccggctgtat accagcattc acaatacatt tttcccccaa aagcaacaac
      481 tcccgttcac tcccggtggc actcactgcc cggaacctga tgtcaccctt cgggacttaa
      541 cgctttcccc gggctaccac agtgattatg cccctcaacc cattccggag atggacagtt
      601 ctgttgttgt tccccccact aatgaaaaca cctcgcctcc ggaagatact cccgacaaca
      661 ctccggccgg aggcaatacc ggacaagcgg aaaagaccag aaacgcagga ctcacaccca
      721 ttcctgaaaa acgttcagga atgcccccgg aacatctgcg gtttgccacg gggtttccat
      781 cgcaaccgaa gattgccggt cccagagaaa agccgatgcg caccgtgcat ccggataaaa
      841 tctaccgtga aattatatgg ttctgctcca gttacctgct gaaatccgga ccagaggcta
      901 cgaggacgat tatcaactca gtattctctg aatgggccag cataaacaat gattacccct
      961 cccccttttc gtgggtggac agcagggaca gtggacagtg tgactggtta tggaacgcca
     1021 tgcaggtccg gtgtgtggga accccgctaa atccccttac cccggagcag aaatactggt
     1081 tcgcctgcgc cacgtttgat aactgggaag gctggaatga gcaacaggta cagtttttac
     1141 tgaaaagtaa tcccagacga aacagagcga agtttacgat aagtcccttc ccggcactca
     1201 gggttaaaca gcataaagct gttcttcttg atgagctgaa aagtgcccgg gagcaacaaa
     1261 aaaggcgtga tgagcgtgct gatggttccg tcccgctgaa actgtccgga aaaatccaca
     1321 aacagcttga aagtattgcc cgaagtcgtg gtgtcccccc aaaaaaaatg ctgaatgaaa
     1381 tgattgagca ggcgcaccgg gattttgtgg caaatgagca gcataagacc cggagttaaa
     1441 gacggcaggg tccccgatta ccaattctgc agcgtactca acaaatgtat ccggccctta
     1501 cctgtgaggg acacgaaaat tatacattga gcatgagtgg ataatcccgg gtgagagtct
     1561 cccgttttta cactattcac taaaggtgag gcatatcatt cagtctctct gaattcttca
     1621 tttcagtaac ttcagctacc gtcgtttcat ctctcccacg aaccggagcc gcaacttgtt
     1681 gcatcacagg gctgacaagt gaactgaaat ccgcacctta atattctgac tggctgcgta
     1741 cattcattgc atgaagcggc cggagcggct gagctgaagg tccgttgtga gcgaaaagcg
     1801 gacattaact ccttgctttt tggcactgaa acagagcgaa gcagcatgta agctgttacg
     1861 gatgcgcagc ggtgacaaca gcaacttcaa ttaaggaaat gctcttggtt ttcggggaga
     1921 cttttcgagc gatatatgat aatgaagaca ctaaaaaact gaacagaaac gtctagctca
     1981 atatgacttc tgttcaggta taaatggtct atttatgtat tctctcatca aatccttact
     2041 gaatactctc gaagatggct aacgccccct ctcttaacct tcatagttca tgccgccaag
     2101 atacgaaggt atcatcatta atgatttaac cctttctttt tctttttcgg tgagatgacg
     2161 acatgcaaat gggtaaatat tgatcaaaaa tgtcttgagt tcgtcaaatg cctcatctga
     2221 atatagagtc ccagatctta actcattcat tattgtcatg acccatttta taaacacagt
     2281 tggcatcatt acatcagcta tatcttccca gccatcaata aggccatggt ttgttttcta
     2341 acatactgcc ggagtgcttg gtcgcagttg cctcgacacg tcgaaaaaat atgttcatta
     2401 tactctggtt ctttttcgta gtcggtgcgg attctttgcc atatagtgat aattcccttg
     2461 tcgtcttgag acagaagctc tttcttgcaa actttgcatt ttaagctagg tttatcggaa
     2521 aatattcttg cgggtttggg gttttctgtt ttccactcag ataacgactt tgggaagtac
     2581 ctttcggcaa tttccaatcc tctggaagag tgtaaaagtt ccctctctat tttctcttta
     2641 tcgaaaacct gaaattcaat tcgtcctttc aacccgtcaa gtattccaga cagacctgca
     2701 ctagcaagag ttgaatagaa cccgataaat ccttggcagt tattcgctat tactcgatca
     2761 gtaatatttg catcatctcc tggattaact gaatttccac tatgagcttt atgcttacag
     2821 cttaccaacc aacggataat tgtttctccg ccgacaccag tccttttttc ttcggctata
     2881 atatcaaccc caccgtcagc ccctctatct gggtcagata taattttata tcccagaaaa
     2941 gctaagaagt cgcgagaaaa aagttcaaat gtgtcttgca gccctgttgc tttatgagcc
     3001 tctggtattt ctttgaagtc taatactggc atttttatgg ggtccttaat ttgttaatgc
     3061 ttattgggtg gaaagaaccg tatttaaaca cggactttct gcctctgtaa atttaatatg
     3121 tagaacactc atcacttttt ataactgaca ataggatatt tagacgtctg tttttggcac
     3181 agggctgctg ttgcagccct gacaacctta gacttcgatc gattcagaga tctctaatgc
     3241 atcatcgact tcaatgccca gataacggac tgtgctttcc agtttcttat ggcccaacag
     3301 aagttggatc acccggagat tcttggtttt cttgtatatc aggtaaggtt ttgttcttct
     3361 catggagtgt gtgctgtaaa gagaagcttc cagaccaagt ttttctactc atccatgaaa
     3421 aattcggttg tattgccggg ttgatatgtg ccggttagtt ccgatccgag accgaaacaa
     3481 gaagtcgttg ctatgcaaat ttccaagttt tatcaatgca gcaacggctt ctcttgtccc
     3541 tttggttatc tcaaattgca caggactacc ggttttctgt tgcagcacag ttgctctgct
     3601 ggaaaccgag ctaccatatg caacatcaga tactttgagg ttgaccagat cacagccacg
     3661 tagcttactg tccaaagcca tattgaacag agccaaatcg cgcgtcttac cttctagttc
     3721 aagcctgatt cggattcccc agatatggga tatctgaagt ggtctttttt ggccgacgat
     3781 acggtctttg ttccacggtg acgtgttcat actcgaatct cctgcaatat gggagatttg
     3841 agtatggttg tctctcatga gcgaggaacg gacattacta acagcattct gtgtgaatca
     3901 ccagggagca ggtcagcgcc actactctat ataatcgcta acgatgacaa gtataattat
     3961 ataagtcttt gcccttagct tttggataaa attgttgaag ttttacgaaa ataatgataa
     4021 acaaaaaaag tagcaataat aatggattta attatggaat ggagattttt aggttctcta
     4081 tcagaggcca ggaaatctgg ctgtagcgga gtttatctta ttgtccataa aggacttttc
     4141 agtcgtgtgg tttacgttgg ggtaagttgt aatgtaggta gaaggataac cgaacattac
     4201 gacggttatc ttagaggaaa tcgaacaatt tacgacgctg gtcacgacga agatgtttat
     4261 cgcttcatgt cagcttataa aattcataac cacacaaaat actatcaagc cttggcgaat
     4321 gattataaga tatgggcatc taccacaatg tattctgatt tgccgaaaaa catgctagca
     4381 aaaagccaaa cctttgatac tgattggcag agcattgcat tagaaaaata tattcctcaa
     4441 ctggtagtat gggcccttcc aatggcaaag tactgttatt caaatgcaag caggattgaa
     4501 agtgtaatcc aatcaaagct cattaaatct tttgatttga gaggtttttt taatataaag
     4561 caattaagca tattgggtaa aattgaatat ccctatatgg aaaaggtcaa agttttcatt
     4621 attaacacac ctgatttaga cccggcatcc cagcttattt ttagcaatct atataacaaa
     4681 aaaactgata acaacttctg taaagagttc cgttcacaat ttaaaagcga gatttttcag
     4741 agggaaagtg aaactcagag aaagcgaact atcagggagc ataaggtatc tctttatgaa
     4801 aattatggca agccttggac tttaaaggaa atggaaaaac ttagagtcat gcttgtcgat
     4861 tttgatttgt ccccgacaga aatatctgag tatctaggaa gagagccgcg ctctatatct
     4921 aaaaaaataa gcgaaaatga taaagttact aattataaat ggcgagagag cgtcgtctgg
     4981 ttgtgacgtt acgttttgat tgaaataacc cccattttgc aaagtccgct cttggcacag
     5041 tgcggacaag ctcactgagc taaaggtccg cagtgagcga actgcggaca tcgtggagac
     5101 aacatgctgc tgggtgcgga tatcccatgg cacgtcatat cgctcgccgt attacttgtt
     5161 atttttttga aacgagaagt tgatacaatt ccttcaggga taaggctaat aactcattct
     5221 tcctgatgag atctttatca gcgtctatac cctctgcgca tgattcattt aaatagttca
     5281 gtgtttcctg atcatcatcc aagcattcat agctgcctga ataaaattca tcgcaacttg
     5341 ctttgttatt tttgtcaatg taaccaatgc attgaatcac gcctttaaca actttagcag
     5401 cagaaaaaac aaagtaactg cagtcattta tatagcgttc aatatcaaaa atcatataaa
     5461 aatagtgcat agagtgtttt tcaatttcaa actcacttga aatcatagct atttcacatt
     5521 caaggtcttt cagaccatct aacattatgg aaagtttaca ttgtttttct tgcacagttt
     5581 catacttaaa tgactctctc ccctttgccc tgacatttat tctttcttca attgcataaa
     5641 tataagcagg atatacacgc gaccacactt ggtttatttt taggattaac cggcctcttt
     5701 tcagcctttc ttttgtagct ataaattctg catatagcgc agcatcctct tcgcaccata
     5761 catcaacttt tcgtttataa cccaacttta attctttccc tacgcattta ggaaacaaag
     5821 gatcaaagtc ctttaagtat tcaaactcac ttagatagaa tcctgtctca taacgattaa
     5881 ataatttcct aaactctttc ccacttaagg ttttcattgt atcaatcact ctattttcat
     5941 aaaagcaaag tcaacaagat aaaacaataa aaactcttac acaaccagct gatgttttta
     6001 aaatagagga taatataaga caagcataac ctcgttcact tcaatgctac tcctatcgtt
     6061 cttttgcgtc acagagcgcc tgccggacag ctgcatcata gaccaaacgg aactgcttcc
     6121 gtcgattaat cagatggcag cgcataaaca cgctcaacaa cccgtttgac tgcttcaatt
     6181 ttaagctctt cggaataacg cttaccgtgt aagtctccct gcaaaacgag ctatcaactt
     6241 ttaaggagtc tctggccccc ctgcttccgc tcctggcaca gagcggactg tcagattaga
     6301 ttcagacctg tatcagccac atatacctgc attccaaata atttcagaca gatattatcg
     6361 ttgtgaatcc cccgccacct ttccggtgcg cggggttttg tcttttttca ccgggaatac
     6421 atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct ccctgcctta
     6481 ctcactttcc cgtcacatcc tggaacacct gcgcaaactc accagtcacg aacccgtgat
     6541 tggcattatg ggtaaaagcg gggccggtaa atcctcactc tgtaatgcac tgtttcaggg
     6601 ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg gaagtgcggc gcttccgtct
     6661 gagtggccac ggtcacagca tggttatcac tgacctgccc ggggtgggcg agagccggga
     6721 cagggatgca gagtatgaag ccctgtaccg tgacattctg cccgaactgg acctggtgct
     6781 gtggctgatt aaagccgatg accgtgccat gtctgtggat gagtatttct ggcgacacat
     6841 cctgcaccag ggacatcagc aggtgctgtt tgtggtgaca caggccgaca aaacggagcc
     6901 ctgccatgaa tgggatatgg ccggtattca gccttctccc gcacaggcac agaatattcg
     6961 cgaaaaaacg gaggcggtat tccgtctgtt ccggcccgta catccggttg tggccgtatc
     7021 ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag cgcttcccga
     7081 ccatgccgcc agtcccctga tgacccgcct gcaggacgag ctgcgcacgg aatctgtccg
     7141 ctctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca cggcggagag
     7201 cgtctgtgtt gcctctgttg cccgtacggt tctgcgtgcc gtccgtgaca cggtggtctc
     7261 tgttgcccgc gctgtatgga actggatttt cttctgaaca ctctgccagg aaaatgcctg
     7321 attacattgc gttaaccaac tgaaaacatg acacaaacac ctcatgctcc tcctgtttct
     7381 cttgcgtgac cgccttgcaa tcaatagaat aaaacgatcg ataaaacaga tatcgatcgt
     7441 tactatcgat cgtttatagt ggttaatggt ctgcgaaaaa gcagttgaat ctcttcatca
     7501 tgcagaacgg aattgcacaa aacggactga tacttctctg gcggatgaga gaggggaagc
     7561 atttatgtcc gggaaaaacc actgtatctg accatgctgt tttgtacctg ccggtatcca
     7621 catttgtggg taccggcttt tttattcacc ctctgtaagg aaaagctgat gaaacgacat
     7681 ctgaacacca gctacaggct ggtatggaat cacattacgg gcaccctggt ggtggcctcc
     7741 gaactggccc gctcacgggg aaaacgcgcc ggtgtggcgg ttgcactgtc tcttgctgct
     7801 gtcacatcag tcccggcact ggctgctgac acggttgtac aggcgggaga aaccgtgaac
     7861 gacggaacac tgacaaatca tgacaaccag attgtcctcg gtacggccaa cggaatgacc
     7921 atcagtaccg ggctggagta tgggccggat aacgaggcca ataccggcgg acaatggata
     7981 caaaatggcg gtatcgccaa caacactact gtcaccggtg gtggtcttca gagagtgaat
     8041 gccggaggaa gcgtttcaga cacggttatc agtgccggag gcggacagag ccttcagggg
     8101 caggcagtga acaccacgct gaacggcggt gagcagtggg tacatgaagg cgggattgca
     8161 acgggtaccg tcattaatga gaagggctgg caggccatca aatccggcgc agtggcaacc
     8221 gacacggttg tgaataccgg cgcggaaggg ggaccggatg cggaaaatgg tgataccggg
     8281 cagaccgtct acggagatgc cgtacgcacc accatcaata aaaatggtcg tcagattgtg
     8341 gctgctgaag gaacggcaaa taccactgtg gtttatgccg gcggcgacca gactgtacat
     8401 ggtcacgcac tggataccac gctgaatggg gggtaccagt atgtgcacaa cgacggtacg
     8461 gcgtctggca ctgttgtaaa cagtgacggc tggcagatta tcaaggaagg tggtctggcg
     8521 gatttcacca ccgttaacca gaaaggcaaa ctgcaggtga acgccggtgg tacagccacg
     8581 aatgtcaccc tgaagcaggg aggcgcactg gtcaccagta ctgcagcaac tgttcttggc
     8641 agcaaccgtc tggggaattt cacggtggaa aacggtaagg ctgacggtgt tgttctggaa
     8701 tccggcggtc gtctggatat actggagagc cattcagcac agaataccct ggtggatgac
     8761 ggcggtaccc tggcagtgtc tgccggcggt aaggcgacag gtgtcaccat gacatccggt
     8821 ggtgccctga ttgcagacag tggtgccact gttgagggga ccaatgccag cggtaagttc
     8881 agtattgatg gtatctccgg tcaggccagc ggcctgctgc tggaaaatgg cggcagcttt
     8941 acggttaatg ccgggggaca ggccggtaac accactgtcg gatatcgtgg aacactgacg
     9001 ctggctgccg ggggaagtct gagtggcaga acacagctca gtaaaggcgc cagcatggta
     9061 ctgaatggtg atgtggtcag taccggcgat attgttaacg caggagagat tcactttgat
     9121 aatcagacga caccggatgc cgcgctgagc cgtgctgttg caaaaggcga ctccccggta
     9181 acgttccata aactgaccac cagtaacctc accggtcagg ggggcaccat caatatgcgt
     9241 gttcgccttg atggcagcaa tgcctctgac cagctggtga ttaatggtgg ccaggcaacc
     9301 ggcaaaacct ggcttgcgtt cacaaatgtc ggaaacagta acctcggggt ggcaaccacc
     9361 ggacagggta tccgggttgt ggatgcacag aatggtgcca ccacagaaga aggtgcgttt
     9421 gccctgagcc gcccgcttca ggccggcgcc tttaactaca ccctgaaccg tgacagcgat
     9481 gaagactggt acctgcgcag tgaaaatgct tatcgtgctg aagtccccct gtatgcctcc
     9541 atgctgacac aggcaatgga ctatgaccgg attctggctg gctcccgcag ccatcagacc
     9601 ggtgtaaacg gtgaaaataa cagcgtccgt ctcagcattc agggcggtca tctcgggcac
     9661 gataacaacg gcggtattgc ccgtggggcc acgccggaaa gcagcggcag ctatggcttc
     9721 gtccgtctgg agggtgacct gctcagaaca gaggttgccg gtatgtctct gacgacaggg
     9781 gtgtatggtg ctgcaggcca ttcttccgtt gatgttaagg atgatgacgg ctcccgcgcc
     9841 ggcacggtcc gggatgatgc cggcagtctg ggcggatacc tgaatctggt acacacatcc
     9901 tccggcctgt gggctgacat tgtggcacag ggaacccgcc acagcatgaa agcatcatcg
     9961 gacaataacg acttccgcgc ccggggctgg ggctggctgg gctcactgga aaccggtctg
    10021 cccttcagta tcactgacaa tctgatgctg gagccacaac tgcagtacac ctggcagggg
    10081 ctctccctgg atgacggcca ggataacgcc ggttatgtga agttcgggca tggcagtgca
    10141 caacatgtgc gtgccggctt ccgtctgggc agccacaacg atatgaactt tggtaaaggc
    10201 acctcatccc gtgacaccct gcgcgacagt gcaaaacaca gcgtgagtga attgccggtg
    10261 aactggtggg tacagccttc tgttatccgc accttcagct cccggggtga catgagcatg
    10321 ggtacagccg cagccggcag taacatgacg ttctcaccgt cccagaatgg cacgtcactg
    10381 gacctgcagg ccggactgga agcccgtgtc cgggaaaata tcaccctggg cgttcaggcc
    10441 ggttatgccc acagcgtcag cgacagcagc gctgaaggtt ataacggtca ggccacgctg
    10501 aatgtgactt tctgataatc aggtcactcc ccttcaggcc ccggtcatca tgaccggggc
    10561 cttttactta cagggagcaa cagatatgcc gggttgtaca tcacgtttat tgcccgaagg
    10621 ccctttctca cgcaaccagg ctcttgccgt cacaacagct taccttaatg tgcttattga
    10681 agatgaccag ggaacgcatt tccggctggt gatccgcaac gctgaaggcc agttgcgctg
    10741 gcggtgctgg aattttgaac ctgatgccgg aaaacagcta aatccgtatc tcgccagtga
    10801 gggaattctc aggcaataaa cgtcattcac ttgatccatc aggccgcgtc ttctccgaaa
    10861 gacgcggctt tttcatttat accgcttaat cattcataag gagccaatta tgcgattagc
    10921 cagccgtttt ggttatgtaa atcagatccg ccgggagcgc ccgctgacac gcgaagaact
    10981 gatgtaccac gtcccgagta tttttgcgga agaccggcac acctcccgca gtgaacggta
    11041 tgcgtacatt cccaccatca ccgtcctgga aaatctgcag cgggaaggct ttcagccgtt
    11101 cttcgcctgc cagacccgtg tgcgcgacca gagccgccgg gaatatacca aacacacgct
    11161 gcgtctgcgg cgggccggac agataaccgg tcagcatgtg cctgaaatta ttctgctcaa
    11221 ctcccatgac ggttcatcca gctaccagat gttacccgga tattttcgtg ccatttgtac
    11281 caatggcctg gtctgcggtc agtcgctggg agaagtccgg gtgccacacc ggggaaacgt
    11341 ggtggacagg gtcatagaag gtgcttacga agtggtgggc gtgtttgacc ggattgagga
    11401 aaagcgtgat gccatgcagt cgctggtcct gccgccaccg gcactccagg cgctggcaca
    11461 ggcggcgctg acttaccgtt atggtgatga acatcagccc gtcaccactg ccgacattct
    11521 gacgccacga cgccgggagg attacggtaa ggacctgtgg agtgcttatc agaccatcca
    11581 ggaaaatatg ctgaaaggcg ggatttccgg tcgcagtgcc agaggaaaac gtatccatac
    11641 ccgggccatt cacagcatcg ataccgacat taagctcaac cgggcgttgt gggtgatggc
    11701 agaaacgatg ctggagagca tgcgctgatg ccttttccct gaaagcgcag ttctgactca
    11761 tgtccgtccc ggcccgcgac ttcttccatt catttacttt ttataaggaa taatctcatg
    11821 acaaccgtct cgcataattt caccacacct tctgtttccg taaccactgc atcagggaat
    11881 aaccagtctc agttggttgc caccatagtc cctgatgaac agcgcatcag cttctggccg
    11941 cagcactttg gcctcattcc acagtgggtc accctggagc cccgtatctt cggctggatg
    12001 gaccgtcttt gcgaaaacta ctgcgatggt atctggaatc tgtacaccct gaacaacggc
    12061 ggggcattta tggcccccga accggatgat gatgatggag aaacatgggt actgttcaat
    12121 gccatgaacg gtaaccgcgc tgaaatgagc ccggaagctg ccggtattgc cgcctgtctg
    12181 atggcgtaca gccatcacgc ctgtcgtacg gagtgttatg ccatgacggt ccattattac
    12241 cggctgcggg attacgccct gcagcatccg gaatgcagcg ccattatgcg catcatcgac
    12301 tgaatggagg cagggacaat gcagcagctt tcctttctgc ccggagagat gacgcccggc
    12361 gagcgcag
//
LOCUS       DAZFFI010000132        12162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFFI010000132 DAZFFI010000000
VERSION     DAZFFI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.132"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1078
                     /gene="emrK"
                     /locus_tag="ACHJ12_004211"
     CDS             <1..1078
                     /gene="emrK"
                     /locus_tag="ACHJ12_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4991917.1"
                     /translation="GAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDT
                     NYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQ
                     SLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQ
                     PQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWV
                     NANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNW
                     IKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVNSMPAYTS
                     KALVIDTSPIEKEISNIISHNGQL"
     gene            1078..2616
                     /gene="emrY"
                     /locus_tag="ACHJ12_004212"
     CDS             1078..2616
                     /gene="emrY"
                     /locus_tag="ACHJ12_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4991918.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(2724..4052)
                     /gene="dsdA"
                     /locus_tag="ACHJ12_004213"
     CDS             complement(2724..4052)
                     /gene="dsdA"
                     /locus_tag="ACHJ12_004213"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4991919.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4070..4288)
                     /locus_tag="ACHJ12_004214"
                     /pseudo
     CDS             complement(4070..4288)
                     /locus_tag="ACHJ12_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            4519..5514
                     /locus_tag="ACHJ12_004215"
     CDS             4519..5514
                     /locus_tag="ACHJ12_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4991920.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLGAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            complement(5522..6955)
                     /gene="cscA"
                     /locus_tag="ACHJ12_004216"
     CDS             complement(5522..6955)
                     /gene="cscA"
                     /locus_tag="ACHJ12_004216"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO4991921.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            7171..8085
                     /locus_tag="ACHJ12_004217"
     CDS             7171..8085
                     /locus_tag="ACHJ12_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO4991922.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            8157..9404
                     /locus_tag="ACHJ12_004218"
     CDS             8157..9404
                     /locus_tag="ACHJ12_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO4991923.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(9846..9920)
                     /locus_tag="ACHJ12_004219"
     tRNA            complement(9846..9920)
                     /locus_tag="ACHJ12_004219"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9885..9887),aa:Arg,seq:cct)
     gene            complement(9996..10928)
                     /locus_tag="ACHJ12_004220"
     CDS             complement(9996..10928)
                     /locus_tag="ACHJ12_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4991924.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            11222..11977
                     /gene="mlaA"
                     /locus_tag="ACHJ12_004221"
     CDS             11222..11977
                     /gene="mlaA"
                     /locus_tag="ACHJ12_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4991925.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         3030 a         2771 c         2965 g         3396 t
ORIGIN      
        1 aggtgcctat gcctattggt caatggaatt agaagacatg attagtacag atgacgccta
       61 tgtcacgggg aatgcagatc caatttctgc acaagtctca ggtagtgtca ctgtcgttaa
      121 tcataaagat acgaactacg ttcgacaagg tgacatttta gtttcactgg ataaaactga
      181 tgccactatc gcactcaata aagctaaaaa taatctggca aatattgttc ggcaaacgaa
      241 taaactatac ttacaggata aacaatacag tgccgaagtc gcttcagcac gtattcagta
      301 tcaacaatct ttagaagatt ataaccgtcg agtgccgtta gcgaagcagg gggttatttc
      361 aaaagaaacg ctggagcata ccaaagatac gttaataagt agcaaagcgg cattgaatgc
      421 cgctatccag gcttataaag cgaataaagc tttagtaatg aacacaccat taaaccgtca
      481 gccacaagtc gttgaagcgg cggatgcaac taaagaagcc tggttggcgc ttaaacgtac
      541 ggatattaag agtccggtta ccggctatat tgcccagcga agtgttcagg tcggcgaaac
      601 agtgagcccc ggacaatcgt taatggctgt cgtaccggca cgtcaaatgt gggttaatgc
      661 caactttaaa gaaacacaac ttacggatgt acggattggt caatcggtca atattatcag
      721 cgatctttat ggtgaaaatg ttgtgtttca tggtcgggtg acagggatca atatgggaac
      781 cggcaatgcg ttctccttat tacctgcaca aaatgcgaca gggaactgga tcaaaatcgt
      841 tcagcgtgta ccggttgaag tttctcttga tccaaaagaa ctcatggaac atcccttgcg
      901 tattggttta tcgatgacag caactattga tacgaagaac gaagacattg ccgagatgcc
      961 tgagctggct tcaaccgtga actccatgcc ggcttatacc agtaaggctt tagttatcga
     1021 taccagtccg atagaaaaag aaattagcaa cattatttcg cataatggac aactttaatg
     1081 gcaatcacta aatcaactcc ggcaccatta accggtggga cgttatggtg cgtcactatt
     1141 gcattgtcat tagcgacatt tatgcaaatg ttggattcca ctatttctaa cgtcgcaata
     1201 ccgacaatat ctggctttct gggagcatca acagacgaag gcacctgggt tatcacctcg
     1261 tttggtgtag caaatgccat tgcgatccct gttactggca ggttggcaca aagaataggc
     1321 gaattaagat tatttttact ttcagtaacc tttttttcgc tgtcttcatt aatgtgtagc
     1381 ctatcgacca atcttgatgt gctgatattt tttagagtcg ttcaggggtt aatggcgggg
     1441 ccgttaattc cactgtcaca gagtttatta ttgaggaatt atccaccaga aaaaagaaca
     1501 tttgctctgg cattatggtc aatgaccgtg attatcgctc cgatatgtgg gccgatattg
     1561 ggcggttata tttgcgataa ctttagctgg ggttggatat ttttaatcaa tgtccctatg
     1621 gggattatcg tcctgacatt atgcttaacc ttacttaaag gaagagaaac tgaaacttca
     1681 ccggtaaaaa tgaatctacc aggactgacc ctgttagtgc tcggtgttgg tggcttgcaa
     1741 attatgcttg ataaagggcg cgatctggat tggttcaact cgagtacaat aataatatta
     1801 acagtagtat cagttatttc tctgatctct ttagtcattt gggagtcgac ctcagagaat
     1861 ccgattcttg atctcagttt gtttaagtct cgtaatttca ccattggtat tgtgagtatc
     1921 acctgcgcgt atttatttta ctctggagcg atcgtcctta tgccgcagtt actccaggaa
     1981 acgatggggt ataatgcgat atgggccggg cttgcttatg cgcccatcgg catcatgcca
     2041 ctattgattt cacctttgat aggacgttat ggcaacaaaa tagacatgcg gttgttagtg
     2101 acatttagtt ttttgatgta tgcggtttgc tattactggc gttctgtgac atttatgcca
     2161 acgattgatt ttacaggcat cattttgcca cagtttttcc agggattcgc cgttgcctgt
     2221 ttctttttac ccttaacaac gatttcgttt tcaggcttgc cagataataa atttgccaat
     2281 gcctcgagta tgagtaattt ttttcgtacc ttgtcaggat cagttggtac gtcgttgaca
     2341 atgacgctgt ggggacgacg cgaatcgtta caccatagtc agttgacagc aaccatcgat
     2401 caatttaacc ccgtgtttaa ttcatcgtca caaattatgg ataaatacta tggttcgctt
     2461 tcaggagttc ttaatgaaat taataatgaa ataacccagc agtcactttc tatttctgca
     2521 aatgagattt tccgtatggc ggctattgct tttatcttac ttacggtttt ggtttggttt
     2581 gcgaaaccgc cgtttacagc gaaaggcatt gggtgatgat aaaaggaggg ggttatagcg
     2641 ggtaagaccg gggatcttaa gtctgcgcca tcggcagcat cgctcaccca gggaaaggat
     2701 tgcgatgctg cgttgaaacg ttattaacgg ccttttgcca gatattgatt catctcttct
     2761 tccggcacca ttccacctcc cgtcgcccac accagatgag tggtattacg cagttgttct
     2821 gcgctgaaac cgtgcatctg ttggtaactt actgatgcac acacgcgctg aggtccggcc
     2881 atacccgcca gtgccgaagg ttcaagacga ataccttctt cctgcgccag ccagccaagc
     2941 atgtcataca tggtttgatc gctaagggta tagaagccat ccagcagacg ctccattgcc
     3001 cgcccgacaa agcctgatgc gcgaccaact gcaaggccat ccgctgcggt aaggttgtcg
     3061 ataccaatat cctgaacaga aatctgatcg tgtaatcctg tatggacgcc taacaacata
     3121 caaggggagt gcgttggttc ggcaaaaaag cagtgaacat gatcgccaaa cgccagttta
     3181 agcccgaatg cgacgccacc aggaccaccg ccaacaccac acggcagata gacaaacaga
     3241 gggttatcag catcgacgat acggccttgc tgggcaaatt gcgctttaag acgctggcca
     3301 gcgacggaat acccaaggaa caacgtgcgg gaattttcgt catcaataaa gaaacagttc
     3361 gggtcagact gcgctgcttt acgtccttcc tcgacggcaa caccataatc ttgctcatat
     3421 tccacgaccg taacgccatg gctgcgcagt ttcgcttttt tccatgcccg ggcatcagca
     3481 gacatatgaa ctgtcacctt aaagccaatg cgggcgctca taatgccgat tgataacccc
     3541 agatttccgg ttgagcccac agcaatgctg tattggctaa agaactgttt aaactccgga
     3601 gaaagcagtt tgctgtagtc atcatcaagc gtcagcaacc ccgcttccag agccagtttt
     3661 tctgcgtgtg ccaggacttc ataaatcccg ccgcgtgctt ttatggagcc ggaaatgggc
     3721 aaatggctat cttttttcag taacagttgc ccgctgatcg gttgctgata ttctttttcc
     3781 agccgttttt gcatagctgg aatggcaacc agttctgatt caataatccc cccagtggca
     3841 gcagtttcag gaaatgcttt tgccagatag ggtgcaaaac gggataagcg cgcatgggcg
     3901 tcctgaacat cctgttcggt caggccaaca taaggtaaac cttcagccaa tgaggtcgtg
     3961 ccaggattaa accaggtggt ttctttaaga gcaaccagat cctttaccaa cggatactgg
     4021 gcgatgagcg agttcatttt agcgttttcc atagtacgtc tctttgcgct tagatgataa
     4081 atgacagcag gaatgtgccc gccagagcga cgactgaagc gataaatgtc gctgtcgtat
     4141 agtatttaaa tgtttcattg agcgtagcgc cgcaatattg cttcactagc cagaaaagcg
     4201 agtccgtaac gatcgtgcag ccaattgcac ctgaaccgat agcaatcgca ataatttccg
     4261 ggctgatgtc gggatacagc ggcagcacaa tgatccatat ttatattttc ttctccaggt
     4321 tggcgatgcc agttggcatg ttttacttac ttgcctatat ggttactcgt tggcgagaag
     4381 agaggcggaa ttggggacaa ttgtccggga attattgtta ttttttattg tattgctgtg
     4441 tcaggtgata gggcgttgga tctcgccagt gacgtctgtt tctgctacag tgcccgtttt
     4501 acggcaaacg gcttgggtat ggcttcatta aaggatgtcg cacgcctggc gggagtgtcg
     4561 atgatgacag tctcccgggt gatgcataat gcagaatctg tgcgtcctgc aacgcgtgac
     4621 cgcgtattgc aggcaatcca gaccctgaat tatgttcctg atctttccgc ccgtaagatg
     4681 cgcgctcaag gacgtaagcc gtcgactctc gccgtgctgg cgcaggacac ggctaccact
     4741 cctttctctg ttgatattct gcttgccatt gagcaaaccg ccagcgagtt cggctggaat
     4801 agttttttaa tcaatatttt ttctgaagat gacgctgccc gcgcggcacg tcagctgctt
     4861 gcccaccgtc cggatggcat tatctatact acaatggggc tgcgacatat cacgctgcct
     4921 gagtctctgt atggtgaaaa tattgtattg gcgaactgtg tggcggatga cccagcgtta
     4981 cccagttata tccctgatga ttacactgca caatatgaat caacacagca tttgctcggg
     5041 gcgggctatc gtcaaccgtt atgcttctgg ctaccggaaa gtgcgttggc aacagggtat
     5101 cgtcggcagg gatttgagca ggcctggcgt gatgctggac gagatctggc tgaggtgaaa
     5161 caatttcaca tggcaacagg tgatgatcac tacaccgatc tcgcaagttt actcaatgac
     5221 cacttcaaat cgggcaaacc agattttgat gttctgatat gtggtaacga tcgcgcagcc
     5281 tttgtggctt atcaggttct tctggcgaag ggggtacgaa tcccgcagga tgtcgccgta
     5341 atgggctttg ataatctggt tggcgtcggg catctgtttt taccgccgct gaccacaatt
     5401 cagcttccac atgacattat cgggcgggaa gctgcattgc atattattga aggtcgtgaa
     5461 gggggaagag tgacgcggat cccttgcccg ctgttgatcc gttgttccac ctgatattat
     5521 gttaacccag tagccagagt gctccatgtt gcagcacagc cactccgtgg gaggcataaa
     5581 gcgacagttc ccgttcttct ggctgcggat agattcgact actcatcacc gcttccccgt
     5641 cgttaataaa tacttccacg gatgatgtat cgataaatat ccttagggcg agcgtgtcac
     5701 gctgcgggag gggaatacta cggtagccgt ctaaattctc gtgtgggtaa taccgccaca
     5761 aaacaagtcg ctcagattgg ttatcaatat acagccgcat tccagtgccg agctgtaatc
     5821 cgtaatgttc ggcatcactg ttcttcagcg cccactgcaa ctgaatctca actgcttgcg
     5881 cgttttcctg caaaacatat ttattgctga ttgtgcgggg agagacagat tgatgctgct
     5941 ggcgtaacga ctcagcttcg tgaaccgggc gttgtagaag tttgccattg ctctctgata
     6001 gctcgcgcgc cagcgtcatg cagcctgccc atccttcacg ttttgagggc attggcgatt
     6061 cccacatatc catccagccg ataacaatac gccgaccatc cttcgctaaa aagctttgtg
     6121 gtgcataaaa gtcatgcccg ttatcaagtt cagtaaaatg cccggattgt gcaaaaagtc
     6181 gtcctggcga ccacattccg ggtattacgc cactttgaaa gcgatttcgg taactgtatc
     6241 cctcggcatt cattccctgc ggggaaaaca tcagataatg ctgatcgcca aggctgaaaa
     6301 agtccggaca ttcccacata tagctttcac ccgcatcagc gtgggccagt acgcgatcga
     6361 aggtccattc acgcaacgaa ctgccgcgat aaagcaggat ctgccccgtg ttgcctggat
     6421 ctttcgcccc gactaccatc caccatgtgt cggcttcacg ccacacttta ggatcgcgga
     6481 agtgcatgat tccttctggt ggagtgagga tcacaccctg tttctcgaaa tgaataccat
     6541 cccgactggt agccagacat tgtacttcgc gaattgcatc gtcattacct gcaccatcga
     6601 gccagacgtg tccggtgtag ataagtgaga ggacaccatt gtcatcgaca gcactacctg
     6661 aaaaacaccc gtctttgtca ttatcgtctc ctggcgctaa cgcaataggc tcatgctgcc
     6721 agtggatcat atcgtcgctg gtggcatgtc cccagtgcat tggcccccag tgttcgctca
     6781 tcggatgatg ttgataaaac gcgtgataac gatcgttaaa ccagatcagg ccgtttggat
     6841 cgttcatcca cccggcagga ggcgcgaggt gaaaatgggg atagaaagtg ttaccccggt
     6901 gctcatgaag ttttgctagg gcgttttgcg ccgcatgcaa tcgagattgc gtcattttaa
     6961 tcatcctggt taagcaaatt tggtgaattg ttaacgttaa cttttataaa aataaagtcc
     7021 cttactttca taaatgcgat gaatatcaca aatgttaacg ttaactatga cgttttgtga
     7081 tcgaatatgc atgttttagt aaatccatga cgattttgcg aaaaagaggt ttatcactat
     7141 gcgtaactca gatgaattta agggaaaaaa atgtcagcca aagtatgggt tttaggggat
     7201 gcggtcgtag atctcttgcc agaatcagac gggcgcctac tgccttgtcc tggcggcgcg
     7261 ccagctaacg ttgcggtggg aatcgccaga ttaggcggaa caagtgggtt tataggtcgg
     7321 gtgggggatg atccttttgg tgcgttaatg caaagaacgc tgctaactga gggagtcgat
     7381 atcacgtatc tgaagcaaga tgaatggcac cggacatcca cggtgcttgt cgatctgaac
     7441 gatcaagggg aacgttcatt tacgtttatg gtccgcccca gtgccgatct ttttttagag
     7501 acgacagact tgccctgctg gcgacatggc gaatggttac atctctgttc aattgcgttg
     7561 tctgccgagc cttcgcgtac cagcgcattt actgcgatga cggcgatccg gcatgccgga
     7621 ggttttgtca gcttcgatcc taatattcgt gaagatctat ggcaagacga gcatttgctc
     7681 cgcttgtgtt tgcggcaggc gctacaactg gcggatgtcg tcaagctctc ggaagaagaa
     7741 tggcgactta tcagtggaaa aacacagaac gatcgggata tatgcgccct ggcaaaagag
     7801 tatgagatcg ccatgctgtt ggtgactaaa ggtgcagaag gtgtggtggt ctgttatcga
     7861 ggacaagttc accattttgc tggaatgtct gtgaattgtg tcgatagcac gggggcggga
     7921 gatgcgttcg ttgccgggtt actcacaggt ctgtcctcta cgggattatc tacagatgag
     7981 agagaaatgc gacgaattat cgatctcgct caacgttgcg gagcgcttgc agtaacggcg
     8041 aaaggggcaa tgacagcgct gccatgtcga caagaactgg aatagtgaga agtaaacggc
     8101 gaagtcgctc ttatctctaa ataggacgtg aattttttaa cgacaggcag gtaattatgg
     8161 cactgaatat tccattcaga aatgcgtact atcgttttgc atccagttac tcatttctct
     8221 tttttatttc ctggtcgctg tggtggtcgt tatacgctat ttggctgaaa ggacatctag
     8281 ggttgacagg gacggaatta ggtacacttt attcggtcaa ccagtttacc agcattctat
     8341 ttatgatgtt ctacggcatc gttcaggata aactcggtct gaagaaaccg ctcatctggt
     8401 gtatgagttt catcctggtc ttgaccggac cgtttatgat ttacgtttat gaaccgttac
     8461 tgcaaagcaa tttttctgta ggtctaattc tgggggcgct attttttggc ttggggtatc
     8521 tggcgggatg cggtttgctt gatagcttca ccgaaaaaat ggcgcgaaat tttcatttcg
     8581 aatatggaac agcgcgcgcc tggggatctt ttggctatgc tattggcgcg ttctttgccg
     8641 gcatattttt tagtatcagt ccccatatca acttctggtt ggtctcgcta tttggcgctg
     8701 tatttatgat gatcaacatg cgttttaaag ataaggatca ccagtgcgta gcggcagatg
     8761 cgggaggggt aaaaaaagag gattttatcg cagttttcaa ggatcgaaac ttctgggttt
     8821 tcgtcatatt tattgtgggg acgtggtctt tctataacat ttttgatcaa caactttttc
     8881 ctgtctttta ttcaggttta ttcgaatcac acgatgtagg aacgcgcctg tatggttatc
     8941 tcaactcatt ccaggtggta ctcgaagcgc tgtgcatggc gattattcct ttctttgtga
     9001 atcgggtagg gccaaaaaat gcattactta tcggagttgt gattatggcg ttgcgtatcc
     9061 tttcctgcgc gctgttcgtt aacccctgga ttatttcatt agtgaagttg ttacatgcca
     9121 ttgaggttcc actttgtgtc atatccgtct tcaaatacag cgtggcaaac tttgataagc
     9181 gcctgtcgtc gacgatcttt ctgattggtt ttcaaattgc cagttcgctt gggattgtgc
     9241 tgctttcaac gccgactggg atactctttg accacgcagg ctaccaaaca gttttcttcg
     9301 caatttcggg tattgtctgc ctgatgttgc tatttggcat tttcttcttg agtaaaaaac
     9361 gcgagcaaat agttatggaa acgcctgtac cttcagcaat atagacgtaa actttttccg
     9421 gttgttgtcg atagctctat atccctcaac cggaaaataa taatagtaaa atgcttagcc
     9481 ctgctaataa tcgcctaatc caaacgcctc attcatgttc tggtacagtc gctcaaatgt
     9541 acttcagatg cgcggttcgc tgatttccag gacattgtcg tcattcagtg acctgtcccg
     9601 tgtatcacgg tcctgcgaat tcatcaagga atgcattgcg gagtgaagta tcgagtcacg
     9661 ccatatttcg ctatcaggat tctgtgtgat ggttacatcg cccggctcag ggctgtttag
     9721 tcatcagcgc tttctgacag tgctgagatt tcaacctgtt gcagtaaaaa tgagtagata
     9781 taaggcaagt gtgctgccaa acccatcttt tacggggtga aggtagattt cgtttgaagg
     9841 gtatctggtg tcccctgcag gaatcgaacc tgcaattagc ccttaggagg ggctcgttat
     9901 atccatttaa ctaagaggac aatgcggcat gagtataccc gctaatggac tgcggggtaa
     9961 gtacgctgcc gctcgattgc ttaaaccctc gccatttatg ctgggttttt atcatttttc
    10021 ttaatgtttt ccgcacgttc tgctttttgg cgtgcttctg ctttacgctt gttgctcatg
    10081 tcgttacgaa tctgtgcatg actcattaac gcgaagataa aggtgccgcc gcagatgttc
    10141 cccgctaaag taggtagtgc gaagggccag atgaaatcgc tccagtgcag cgtgccgtta
    10201 aacaccagat agaggatttc aacagaaccg acaacgatgt gggtggtgtc acccagtgca
    10261 ataagccagg tcatcaatat aatcaccaca atctttgccg cacccgcagc aggaaacatc
    10321 caaaccatag tggcgatcag ccagccggaa atgatcgcgt tggcaaacat ctcgctgggg
    10381 gtgttcttca tcacatccat gccgattttg acaaatgcat cgcgagtttc ttcattgaag
    10441 ataggcatat attcaaatgc ccatgcagca atacctgtcc cgagaatatt acccagcagc
    10501 acgacgcccc ataatcgcat aagtaagccg acgttgctca ttgtcggttt ttgcatgacg
    10561 ggtagtaccg cagtcacggt gttttcggta aataattgct ggcgggccat aatgacgata
    10621 ataaaaccaa aggtataacc gagattctcc agtaagaagc tgcctggcac tccttccagt
    10681 tcgacatgaa atatcccttt tgccagtagc gaagcgccca tcgacagacc cgccgcaatg
    10741 gctgaccaca gtagcgccat tgcgtcgcgt tccagctctt tttcaccatc ctggcggata
    10801 tgctcatgaa ttgccatcgc ccgggagggg agtcggtctt catctatttc tatttttttg
    10861 ccgcgctctt tttcttcgct ctcaacttca atttcgtcgc tgtgttgatc aattttgtcg
    10921 ttgtccatgg tctcttctct tgaattagca cgtatagcta aagcgtagcg gcttttttgc
    10981 tcgcaactgg cgggagttac tctgaaaatg tagaaaaggc tgcgtttgcc tttttctgtt
    11041 tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa atcagcaaat
    11101 cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa acaggcggat
    11161 acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca gggagacatt
    11221 tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg ggtgtgcgag
    11281 ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc gcaccatgta
    11341 caacttcaac ttcaatgtat tagacccgta tattgttcga ccggtcgctg tcgcctggcg
    11401 tgattatgtt ccgcaaccgg cgcgtaacgg tttgagcaac tttactggca accttgaaga
    11461 acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga tggtccactt
    11521 tacccgcttt ttcctgaaca ccattttggg gatgggcggt tttattgatg ttgcagggat
    11581 ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc ttggtcatta
    11641 tggcgtgggt tatgggcctt acgtccagtt accgttctac ggtagcttca cgctgcgtga
    11701 tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga cctggccgat
    11761 gtctgtgggt aaatggacgc ttgaagggat agaaactcgt gcgcagttgt tggattccga
    11821 tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt acttccagcg
    11881 tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga acgcgcaagc
    11941 gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa aaggtgagtc
    12001 gcaatactca ccttttttgt tgttatccct caattaccac aagtaaaagg agataaaccg
    12061 acaatgaccc ggacgtaatg atgtaaaaat actgatgttc gtcactgact atttccgata
    12121 ttcaggagtt gaaaaatcaa aaaactggaa aaataatttt aa
//
LOCUS       DAZFFI010000133        12139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFFI010000133 DAZFFI010000000
VERSION     DAZFFI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.133"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..153
                     /locus_tag="ACHJ12_004222"
     CDS             <1..153
                     /locus_tag="ACHJ12_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4991926.1"
                     /translation="VVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFS
                     SSSWPF"
     gene            477..1865
                     /gene="narU"
                     /locus_tag="ACHJ12_004223"
     CDS             477..1865
                     /gene="narU"
                     /locus_tag="ACHJ12_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4991927.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1947..5687
                     /gene="narZ"
                     /locus_tag="ACHJ12_004224"
     CDS             1947..5687
                     /gene="narZ"
                     /locus_tag="ACHJ12_004224"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4991928.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5684..7228
                     /gene="narH"
                     /locus_tag="ACHJ12_004225"
     CDS             5684..7228
                     /gene="narH"
                     /locus_tag="ACHJ12_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4991929.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7228..7923
                     /gene="narW"
                     /locus_tag="ACHJ12_004226"
     CDS             7228..7923
                     /gene="narW"
                     /locus_tag="ACHJ12_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4991930.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7920..8600
                     /gene="narI"
                     /locus_tag="ACHJ12_004227"
     CDS             7920..8600
                     /gene="narI"
                     /locus_tag="ACHJ12_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4991931.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8679..9572
                     /gene="yddE"
                     /locus_tag="ACHJ12_004228"
     CDS             8679..9572
                     /gene="yddE"
                     /locus_tag="ACHJ12_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4991932.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9668..10513)
                     /gene="nhoA"
                     /locus_tag="ACHJ12_004229"
     CDS             complement(9668..10513)
                     /gene="nhoA"
                     /locus_tag="ACHJ12_004229"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO4991933.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10686..11255
                     /gene="yddH"
                     /locus_tag="ACHJ12_004230"
     CDS             10686..11255
                     /gene="yddH"
                     /locus_tag="ACHJ12_004230"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4991934.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11259..11486)
                     /gene="pptA"
                     /locus_tag="ACHJ12_004231"
     CDS             complement(11259..11486)
                     /gene="pptA"
                     /locus_tag="ACHJ12_004231"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO4991935.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11540..12139)
                     /locus_tag="ACHJ12_004232"
                     /pseudo
     CDS             complement(11540..12139)
                     /locus_tag="ACHJ12_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2902 a         3127 c         3139 g         2971 t
ORIGIN      
        1 gttgtaaacc agaggaatat aaaagttaaa aacatgacct tagatgaaat aaacaatact
       61 tactgtaata acgattatta caatcaggca ataagagagg aaccgataga ccttctggac
      121 agatcgtttt cctccagttc atggcctttt tagtgaataa atcatcacta attctataag
      181 tgtctggtct tgttgataat atagttaata tgtaaccctt tagattcacg atctgaaggg
      241 ttgaaacatg acatacgttc aaagattaac ccccgtttga aggttagctt gttagttaca
      301 tttagtaaca catatctggc tacttaatcc ccattctgcc cccgcggtta cgctttctta
      361 tctattcttt caatcagcac ttcacccaat tcatccttaa ttctaataac tggctattgc
      421 gccttacctc ctgttttgtc ttttttcatt accaatgtgt gcatgtgagg aacaatatgg
      481 cactgcaaaa tgagaaaaat agtcgttatc ttttacgcga ctggaaacca gaaaatccgg
      541 ccttctggga aaataaagga aaacatattg ctcgaagaaa cctctggata tcagtcagtt
      601 gtctacttct tgccttctgt gtctggatgc tatttagcgc agttaccgtt aatctcaata
      661 aaatcggttt taattttact accgatcaac tctttttatt aaccgcatta ccctccgttt
      721 ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc tatattcggt ggacgccgat
      781 ggacggtttt tagtactgca atcctgatta ttccttgcgt ctggctcgga attgccgtgc
      841 aaaatccgaa tactcctttt ggggtattta tcgttatcgc tttgctatgc ggttttgcag
      901 gtgcaaactt tgcttcgagc atgggcaata tcagtttctt ctttccaaaa gccaaacaag
      961 ggagcgctct tgggattaat ggcggattag gaaacttagg tgtaagtgta atgcagctgg
     1021 ttgcaccgct ggtcattttt gtacctgtat ttgcctttct cggcgtcaat ggcgtaccgc
     1081 aggccgacgg ttcggtgatg tcgctggcga atgccgcatg gatttgggta ccgctactgg
     1141 cgattgccac gatcgccgcc tggtcaggga tgaatgatat cgccagttca cgcgcctcaa
     1201 ttgccgacca gctccctgtc ttacaacgcc tgcatctctg gctgctgagc ctgctttacc
     1261 ttgccacctt cggttcgttt atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc
     1321 agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg
     1381 cgcggtcggt tggtggtgct atttccgata agttcggcgg cgtgcgggtg acgttgatca
     1441 actttatttt tatggcgatt ttcagtgccc tgctgttcct taccttaccg ggcacaggct
     1501 ccggtaattt catcgccttt tacgccgtat ttatggggct gtttctgact gcgggtctgg
     1561 gaagtggttc tactttccag atgatcgccg tcatctttcg ccagataacc atttatcggg
     1621 taaagatgaa aggcggtagt gatgagcaag ctcagaaaga agccgtcacc gaaacggcgg
     1681 cggctctggg ctttatctca gccattggcg cagtgggcgg cttttttatt ccgcaggcgt
     1741 ttggcatgtc gctcaatatg accggctctc cggtcggcgc gatgaaagtg tttttaatct
     1801 tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg tcggcggaag ttcagccaaa
     1861 aataagagat cgatgcctga tgtacaaccg tgtcgcatcc gacaaggcat cagatcccaa
     1921 ttcattcctg gagcaggagt catgtcatga gtaaactttt ggatcgcttt cgctacttca
     1981 aacaaaaggg cgaaaccttt gccgatggtc acggacaggt gatgcatagc aaccgcgact
     2041 gggaggacag ctatcgccag cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg
     2101 ttaactgtac aggctcctgt agctggaaaa tctacgttaa aaatggtctg gtgacctggg
     2161 aaatccaaca gaccgactac ccgcgcactc gccctgacct gcccaatcat gaacctcgcg
     2221 gctgcccgcg tggcgcaagt tactcctggt atctttacag cgctaaccgc ctgaaatacc
     2281 cgctcattcg taaacgactg attgaactgt ggcgcgaagc cctcaagcaa cacagcgatc
     2341 cggtactggc gtgggcatcg attatgaacg atccgcaaaa gtgcctgagc tacaaacaag
     2401 tgcgtgggcg cggcgggttt atccgctcca actggcagga actaaaccag ctgattgccg
     2461 ccgctaacgt ctggaccatc aaaacctacg gcccggatcg cgttgccggt ttctcgccga
     2521 tcccggcgat gtcgatggtt tcttacgccg ccggaacgcg ttatctgtcg ctgcttggcg
     2581 gcacctgttt aagtttctac gactggtatt gcgacctgcc gcccgcctcg ccgatgacct
     2641 ggggcgagca aaccgacgta ccggaatctg ccgactggta taactccagc tacatcatcg
     2701 cctgggggtc taacgtaccg cagacacgta cgccggacgc ccacttcttt accgaagtac
     2761 gctacaaagg cactaaaacc atcgccatta cccctgacta ctctgaagtg gccaaattgt
     2821 gcgaccagtg gctggcaccg aaacaaggca ctgacagcgc cctggcgatg gcaatgggcc
     2881 atgtgatttt aaaagagttt catctcgata atcccagcga ctactttatc aactactgcc
     2941 gccgctacag cgacatgccg atgctggtaa tgctggagcc tcgcgacgat ggtagctacg
     3001 ttcccgggcg catgatccgc gcatctgacc tggtggatgg actgggcgaa agcaacaatc
     3061 cgcagtggaa aaccgtagca gttaataccg caggtgaatt ggtagtgccg aacggttcga
     3121 ttggtttccg ctggggagaa aaaggcaaat ggaatctgga atccattgcc gccggtacgg
     3181 aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc tgttgcaggc gtggccttcc
     3241 cctactttgg cggcattgaa aatccgcatt ttcgcagcgt aaaacacaat ccggtgctgg
     3301 tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg caacacctgt ccggtggtca
     3361 gcgtttatga tttggtactg gcgaattacg gcctcgatcg cgggctggaa gatgaaaaca
     3421 gtgcgaaaga ttacgctgaa atcaaaccgt acaccccagc ctggggtgag caaattaccg
     3481 gcgtgccgcg ccagtatatt gaaaccatcg cccgtgaatt tgccgatacc gcccataaaa
     3541 cgcatgggcg ctcgatgatt atcctcggcg caggtgttaa ccactggtat cacatggaca
     3601 tgaactaccg cgggatgatc aatatgctga tcttctgcgg ttgtgtcggg caaagcggtg
     3661 gcggctgggc acactatgtc ggccaggaaa aactgcgccc acaaaccggc tggttgccac
     3721 tggcctttgc gctcgactgg aaccgaccac cgcgccaaat gaacagcacc tcgtttttct
     3781 acaatcattc cagccaatgg cgctatgaaa aagtctctgc tcaggagtta ctttcaccgc
     3841 tcgccgatgc cagtaagtac agcggtcatc tgattgattt caacgttcgc gccgaacgta
     3901 tgggctggct accttctgcg ccgcagctgg ggcgtaaccc gctcgggatt aaagctgaag
     3961 ccgacaaggc cggattatcc cccacagaat ttaccgccca ggcgctgaaa tcgggcgatt
     4021 tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca tccgcgtaat ttgtttgtct
     4081 ggcgctctaa cctgcttggc tcctccggca aaggccacga gtatatgcag aagtatctgc
     4141 tggggaccga aagcgggatt cagggcgagg aactcggtgc cagcgacggg atcaaaccgg
     4201 aagaagtcga gtggcaaact gcggcgattg aaggcaagct cgacctgctg gtgacgctcg
     4261 acttccgcat gtccagtacc tgcctgttct ccgatatcgt tctgcccacc gccacctggt
     4321 acgaaaaaga cgatatgaac acctcggata tgcatccgtt tattcatccg ctttctgcgg
     4381 cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat ctacaaaggt atcgccaaag
     4441 cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac cgacgtggta ttacaacccc
     4501 tactgcatga ctctccggca gagctctcac agccgtgtga agtgctcgac tggcgcaaag
     4561 gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat tgtggcggtg gagcgcgact
     4621 accctgctac gtatgaacgc tttacctcgc tcgggccatt gatggacaaa cttggcaatg
     4681 gcggtaaagg gatttcgtgg aatacgcagg atgaaatcga tttcctcggc aaactcaatt
     4741 acaccaagcg tgatggcccg gcacaggggc gtccgctgat tgacaccgcc attgacgctt
     4801 cagaagtgat tctggcactg gcaccggaaa ccaacggtca tgttgcagtc aaagcgtggc
     4861 aggcgctggg cgagatcacc gggcgcgaac atacccatct ggcgctgcac aaagaggacg
     4921 agaagattcg ctttcgcgat attcaggcgc agccgcgtaa aattatctcc agccccacct
     4981 ggtccggtct ggaaagcgat cacgtatcct ataacgcggg atacaccaac gttcatgagt
     5041 taattccgtg gcgcacgctg tcgggacgcc agcagctcta tcaggatcat ccgtggatgc
     5101 gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat cgacacccgt agcgtcagtg
     5161 agatgcgcca gataccgcca aacggcttcc cggaaaaagc acttaacttc ctgacgccgc
     5221 accagaaatg gggcattcac tcaacctaca gtgaaaacct gctaatgctg acgctctctc
     5281 gcggtggacc gattgtctgg atcagcgaaa cagatgcccg agaactgacc attgtcgata
     5341 acgactgggt ggaagtattc aacgccaacg gcgcgctgac ggcccgcgcg gtggtcagcc
     5401 aacgtgtacc gccgggcatg accatgatgt atcacgccca ggaacgcatt atgaatattc
     5461 ctggttcgga agtaactggc atgcgcggcg gcattcataa ctcggtcacc cgcgtttgcc
     5521 cgaaaccaac gcatatgatt ggcggttacg cgcagctggc ctggggcttt aactactacg
     5581 gcaccgtcgg atcgaaccgc gatgagttca tcatgatccg caagatgaag aacgttaact
     5641 ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa aaaatgaaaa tccgttcaca
     5701 agtcggcatg gtgcttaacc tcgacaaatg tatcggctgc catacctgtt cggtgacctg
     5761 taaaaacgtc tggaccgggc gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac
     5821 caaaccgggc attggttatc cgaaaaactg ggaagatcag gaagagtggc aaggcggctg
     5881 ggtccgcgat gtgaatggca agatccgtcc gcgtctgggc agcaagatgg gcgtaataac
     5941 caaaatcttc gccaacccgg tggtgccgca gattgatgat tactacgaac ctttcacctt
     6001 cgactacgaa catttgcata gcgcaccgga aggcaaacat atccctactg ctcgcccgcg
     6061 ttcactgatt gacggcaaac ggatggacaa agtgatctgg ggcccaaact gggaagaact
     6121 gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca tgcaaaagga
     6181 gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt gcgaacactg
     6241 cctcaatccc agttgcgtgg cgacctgccc aagcggcgct atctacaaac gcgaagaaga
     6301 cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca ttagcggttg
     6361 tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat gtatcttctg
     6421 ttatccgcga attgagtcag gccaacccac cgtgtgctca gaaacctgcg tgggtcgcat
     6481 ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt gaggaagcgg cgagcaccga
     6541 gcgcgaagtt gacctctatg aacgtcagtg cgaagtattc ctcgatccac acgatccctc
     6601 agtgatcgag gaagccctga aacaaggtat tccacaaaac gtgattgaag ctgcccagcg
     6661 ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg ctaccgctgc accctgaata
     6721 tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg ccgattcagt cctacgcaga
     6781 tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc atcgaaagcc tgcgtattcc
     6841 ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc ggtccggtac tgcgggcgct
     6901 gaaacggatg atggcgatgc gccactatat gcgttcacaa accgtggaag gcgttactga
     6961 tactcgtgcc atcgacgaag taggcctgag cgtcgcccag gtcgaagaga tgtatcgtta
     7021 cctcgccatt gccaactatg aagatcgttt tgtcatcccg acgagccatc gggaaatggc
     7081 gggcgatgcc ttcgcagaac gcaacggctg cggttttacc tttggcgacg gttgccacgg
     7141 ttcggacagt aaattcaacc tgttcaacag tagccgtatc gatgccatca acatcaccga
     7201 agtgcgcgac aaagcggagg gcgaataatg cagatcctca aagtgatcgg cctgttgatg
     7261 gagtatccgg acgagctgtt gtgggaatgc aaggaggacg cgctggcgtt gatccgcagc
     7321 gacgcgccga tgcttacgga tttcacccac aacctgctta acgcgccgct gctggataaa
     7381 caggccgaat ggtgcgaagt gtttgaccgt ggacgtacca catcgctgct gctgttcgaa
     7441 catgttcatg ccgagtcccg cgatcgcggc caggcaatgg tggacctgct ggcggagtat
     7501 gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg attatctacc gctgtatctg
     7561 gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag gattgctcaa tgttgcgccg
     7621 atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg caccgtggta cgcgttgttt
     7681 gatgctctgc tgcaactggc gggaagctcc ctttcaagtg acagtgtcac aaaacaagta
     7741 aacagcgaag agcgcgatga cacccgccag gcgcttgatg cagtgtggga agaggaacag
     7801 gtgaagttta ttgaagataa cgccacggcg tgtgacagct cgccgcttaa tcaatatcag
     7861 cgacgcttta gccaggatgt cgcgccgcaa tatgtcgaca tcagtgcggg aggtgggaaa
     7921 tgattcagta tctgaacgtc tttttttacg atatctaccc gtacatttgt gcgacggtgt
     7981 ttttcctcgg cagctggctg cgctacgact acgggcagta cacctggcgc gcctcctcaa
     8041 gtcaaatgct cgataaacgc gggatggtga tatggtcgaa tctgttccat atcggcattt
     8101 tggggatttt cttcgggcac ttgttcggca tgttaacgcc gcactggatg tacgcgtggt
     8161 ttttgccagt ggcagcgaaa caactaatgg cgatggtgct cggcggtatt tgcggtgtgt
     8221 tgacattgat tggcggcgca ggactgctgt ggcgcaggct gacaaatcag cgcgtgcgtg
     8281 caacttccac tacgccggat attatcatca tgagcatttt gctgatccag tgtctgctgg
     8341 ggctaagtac cataccgttt tcagcacagt atcctgatgg tagcgaaatg atgaagctgg
     8401 tgggttgggc gcaaagcatt gtcactttcc gtggtggatc gtcagaaatg cttaacggcg
     8461 tagcgttcgt tttccgcctg catctggtat tgggaatgac catcttcctg ctcttcccgt
     8521 tcaccagatt ggtgcatgtg tggagcgcgc cgtttgagta ctttactcgt cggtatcaga
     8581 ttgtgcgttc gcggcgataa tttcaccctg gccggatagc ccttcgctct ccggccactt
     8641 gcaggctatg atagttctcc agtcacagga gaacgttaat gaaaccgcag gtgtaccacg
     8701 tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc cggtgttgtt ttccccgccg
     8761 ataatcttag cgaagcgcaa atgcagctta tcgcccgcga gttaggccat tcggaaactg
     8821 cttttctgct gcacagcgac gacagcgatg tgcgcatccg ctactttacg ccaacggttg
     8881 aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta tgtacgtgcg aaggtgttag
     8941 gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg aaaacatcgc gtgactatcg
     9001 aaaagcacaa cgatgattat cgtatttcgc tggaacaagg tacgccgggc tttgaaccgc
     9061 cgctggaggg tgaaacacgt gaggcgatta tcaacgcgct tcatctcact gaggacgata
     9121 ttctgccagg cttgccaatt caggtggcaa caacaggaca ctcaaaagtg atgatcccgt
     9181 tgaaaccaga agtggatatc gacgcccttt cgcctgatct tgctgcgctg agcgctatca
     9241 gcaaacagat tggctgcaat ggtttcttcc cgttccagat ccgcccaggc aaaaacgaaa
     9301 ccgatggtcg catgttctcg cctgcgattg gtattgtgga agatccggtg actggcaatg
     9361 ccaatggccc gatgggtgca tggttggtac atcacaacgt attgccccac gatggcaagg
     9421 tgctgcgtgt taaaggccat caggggcgcg cattggggcg tgacggcgtg attgaagtga
     9481 cggtgacaat tcgtgataac caaccggaaa aagtcaccat ttcaggcaca gcggtgattt
     9541 tgtttcatgc cgaatgggca attgaaatct gaaatcgttg cctgatgcgc ttcgcctgtc
     9601 aggtctacaa ccgggctaat gtatgccgga taaggcgatt acgccgcatc cggcaacctg
     9661 acataaatta ttttcccgcc tccggatgcg tatcaaacgc cgccatcacc agcgctaact
     9721 catccacagt aaagccatgt ttcgcatcat ccacgcccag accaaactgt tcctgcatca
     9781 cggcatataa cgacaccaca tccggtagat ttcgctgctc caccgcgtgc ccattttcat
     9841 aatgggtaaa atgaaaattg gtcagcgtta gcttgccacc gtccggcaaa tggcggcaca
     9901 tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc cgaccagaaa ttgcccatca
     9961 cataatcgct ttgttgctgc tcgcagagat caaaacggta catcgactgc caatgctgat
    10021 gatgattaaa ctgcaacacc caatcatcac cctcctgcaa caaccgatac tctccgtgtg
    10081 gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc ggttagcgtc tgcccaccga
    10141 aaccgacatc agcaatccat ttttcctctt ccagttccac caacagcaaa cgatgggtgc
    10201 gcggcggtaa tgctggcgga tttgataaca ctacgcgccc taacaagctg cgaacgttaa
    10261 accccagctc gcgtaacacc cgctcaaaca cgccattctg ctcaaaacag taaccgccac
    10321 gacgggctat caccagtttc tcttccagcg attgatcatc aagctgtatt tccctcggca
    10381 gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg tttcaggtgc aatgcacgaa
    10441 gcgtatcaat attgaccgca gcagctcccg accagttaat acgggcaaaa tagtgattca
    10501 gaatgggcgt catcaggcgt ttcccttagt ggttttctca acttataacc tggaatagac
    10561 gccgcaccgg ctattttgtg cgatcgcgga catgcatcac gtttgcaaat cgccaatccc
    10621 gttaatttcg ttacttattg cgttacggac tacgcttaaa aaaaccttct tcgcgaggcg
    10681 ataccatgag ccgattcatc cccatagaat tacaccatgc cagccgtcta ttaaatcatg
    10741 gcccaactgt catgatcacc agtttcgatg agcaatccca gcggcgtaac attatggctg
    10801 cagcctggtc aatgccggtg gagtttgaac cgccacgcgt ggcgattgtg gtagataaat
    10861 cgacatggac cagggagttg attgaacgta acggtaagtt tggcatcgtt atcccgggcg
    10921 ttgcagcaac taactggacg tgggcagtgg gaagcgtgtc gggtcgtgaa gaagataaat
    10981 ttaattgcta tggcattccg gtggtgaaag gtccggtgct tggtttgcca cttgttgaag
    11041 agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc gacttctgcg caacaaaaat
    11101 acgacacgct gtttggcgag gtggtatcag cagcggcaga cgcacgggta tttgtcgaag
    11161 gccgctggca gtttgatgat gataagctca atacgttgca tcatttaggt gctggcacgt
    11221 ttgttactag cggcaagcgt gttacggcag gttaagcatt acatgctgta accgggtttc
    11281 tttatcaaag cctccatttg gggcgcgatt tcggcatccc agatagcttg ccaagattct
    11341 ggttgaatct gctgtagagc aatgcttatc gaactgtctt tactgttcag atggcgaata
    11401 ataacgtcgg taatatctgc agcaagtgct gctttttgtt gttcgtccag ttcacgcgga
    11461 aagcatttaa tgtcgatgtg cggcatgatt gtgctccttt tgaaagatgt cagtaaaatt
    11521 aacacacatt gtgaattttc agtcctttcc attcaaacgt gaaatcaata agttcatcag
    11581 ggacatcgca aacaatatga atacggattt cttctctgcc gtgactcttt tcacaaattg
    11641 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
    11701 tatttagcca cccctggttt cctttcaccg cgaataaata atcgcctccc tgtttttgta
    11761 tcttctctgc tatatctttc tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa
    11821 tatccagcat gttaagaagt tctgggatag ctgtaatctc attagattta tcatccgtct
    11881 tgatctgtcc gatgaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
    11941 ccctgcggcg actcttatca taagaatgcc ggagcgtttt tccatcaatt gcaatgacgt
    12001 ctttatcatc tgaagaatga caatcacgca tccagttaat aaagcactca tgaaattttg
    12061 tagggctgat acaggataca actctggcaa tggtatcgtg aacaggaata ccattttcaa
    12121 aatcaccata ttgcttcaa
//
LOCUS       DAZFFI010000134        12122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFFI010000134 DAZFFI010000000
VERSION     DAZFFI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.134"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..1002
                     /gene="yjjP"
                     /locus_tag="ACHJ12_004233"
     CDS             232..1002
                     /gene="yjjP"
                     /locus_tag="ACHJ12_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4991936.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            993..1466
                     /gene="yjjB"
                     /locus_tag="ACHJ12_004234"
     CDS             993..1466
                     /gene="yjjB"
                     /locus_tag="ACHJ12_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4991937.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1573..2112
                     /gene="dnaT"
                     /locus_tag="ACHJ12_004235"
     CDS             1573..2112
                     /gene="dnaT"
                     /locus_tag="ACHJ12_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4991938.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2115..2852
                     /gene="dnaC"
                     /locus_tag="ACHJ12_004236"
     CDS             2115..2852
                     /gene="dnaC"
                     /locus_tag="ACHJ12_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4991939.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2901..3395
                     /gene="yjjA"
                     /locus_tag="ACHJ12_004237"
     CDS             2901..3395
                     /gene="yjjA"
                     /locus_tag="ACHJ12_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4991940.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSATSELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3649..5940
                     /gene="opgB"
                     /locus_tag="ACHJ12_004238"
     CDS             3649..5940
                     /gene="opgB"
                     /locus_tag="ACHJ12_004238"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4991941.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6079..7101)
                     /gene="lgoD"
                     /locus_tag="ACHJ12_004239"
     CDS             complement(6079..7101)
                     /gene="lgoD"
                     /locus_tag="ACHJ12_004239"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4991942.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7240..8154
                     /gene="lgoR"
                     /locus_tag="ACHJ12_004240"
     CDS             7240..8154
                     /gene="lgoR"
                     /locus_tag="ACHJ12_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4991943.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8369..9730
                     /gene="lgoT"
                     /locus_tag="ACHJ12_004241"
     CDS             8369..9730
                     /gene="lgoT"
                     /locus_tag="ACHJ12_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4991944.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9779..11443)
                     /gene="tsr"
                     /locus_tag="ACHJ12_004242"
     CDS             complement(9779..11443)
                     /gene="tsr"
                     /locus_tag="ACHJ12_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4991945.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11562..12008)
                     /gene="hpaR"
                     /locus_tag="ACHJ12_004243"
     CDS             complement(11562..12008)
                     /gene="hpaR"
                     /locus_tag="ACHJ12_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO4991946.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2941 a         3199 c         2950 g         3032 t
ORIGIN      
        1 tatcctgatt cgtaaaggcc aaacaataaa ttcattatag tgataatatt catgtgatag
       61 tttgtttgcg gcgagagata attcgctttt tatcaccgag cttgtcccga taaccaggat
      121 taatcagcgc aattttgatc ttaacctacc gcaaaacaac cgtgcttcat gatacaaatg
      181 gattctcatt atctgaacaa tacgcagcac gtctatgaca aagggcgagt tatgcaaact
      241 gagcaacagc gagccgtaac acggctttgt atccagtgtg gattatttct tttgcaacat
      301 ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac tgggtcgggc gctgggaatg
      361 gacagtgtcg aaagttctat ctcttcgaac gccatagtgc tgacaactat taaagatggg
      421 caatgcctga catcgacacg taaaaatcac gatcgcggca ttaatatgca tgtggtgact
      481 gaagtccagc acattgtgat tcttgcggag catcatctgc tggattacaa aggcgtagag
      541 aaacgattta gccaaattca gccattacgt tacccaagat ggctggtagc cttaatggtt
      601 ggcctttctt gcgcctgttt ctgtaaactc aataacggtg gctgggatgg tgccgtcatc
      661 accttctttg ccagtacgac cgcgatgtat atccgccagc tgctggcaca acgtcatctt
      721 catccacaga tcaacttttg ccttaccgct ttcgccgcca ccaccatttc cggattgctt
      781 ttgcaactcc ccactttcag caataccccc accattgcga tggccgccag cgttctgctg
      841 ctggtgccgg gctttccgtt gattaatgcc gtcgccgata tgtttaaagg ccacatcaat
      901 accggactgg cacgctgggc gatcgccagt ctgctgacac tggctacctg cgtcggcgtg
      961 gtgatggcac tgacgatttg ggggctacgc ggatgggtgt gatcgaattt ctgtttgcat
     1021 tggcgcagga tatgatcctc gccgccattc ctgcggtcgg ctttgcgatg gtgttcaacg
     1081 ttcccgtgcg ggcgttacgc tggtgtgcgc tgcttggctc gataggtcat ggttcccgaa
     1141 tgatcttgat gaccagcggg ttgaatattg agtggtcaac ctttatggct tctatgctgg
     1201 tcggcaccat tggtattcaa tggtcgcgct ggtatctggc gcatccgaaa gtgtttaccg
     1261 tggcggccgt tatccctatg ttcccgggca tatcggctta taccgcaatg atttcggcgg
     1321 taaaaatcag ccagctaggt tacagcgaac cgttgatgat taccctgtta accaactttc
     1381 ttacagcttc atcgattgtt ggtgcgttat ccatcggtct ttccattcct ggattatggt
     1441 tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta agcacacgga tgagagacag
     1501 cctcctctcc tccgtgtgtt actataaaag ttatctccct tctcgttcat cgttccatat
     1561 ttgagaaaca gtatgtcttc cagagttttg accccggacg tcgttggtat tgacgccctg
     1621 gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct
     1681 aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc tggctgaact gctggcgctg
     1741 gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg acgatcaact ctatcaggaa
     1801 ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg ccatgtatcc ggactggcaa
     1861 cccgatgccg actttatccg cctggcggcg ctatggggcg tggcgctaag agagccggta
     1921 accaccgaag aactggcctc attcattgcc tactggcagg cggaaggtaa agtctttcac
     1981 catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc
     2041 ggactgccga aacgagatgt gaatacggtc agtgaacctg acagccaaat tccaccagga
     2101 ttcagagggt aacgatgaaa aacgttggcg acctgatgca acgcctgcaa aaaatgatgc
     2161 ctgcccatat caaacccgca ttcaaaacgg gtgaagaact actggcctgg caaaaagaac
     2221 aaggggcgat ccgctccgcc gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca
     2281 cctttaaccg ctccggtatt cgtccactgc atcagaactg ctcctttgag aactatcgcg
     2341 ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg ccagtatgtc gaagagtttg
     2401 acggcaacat cgccagcttt atcttttctg gtaagccagg aaccggcaaa aaccatctgg
     2461 cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg
     2521 ccgatattat gtcggcgatg aaagatacct tcaggaatag cggtaccagc gaagaacaac
     2581 tgcttaacga tctgagcaac gttgatctgc tggtgatcga tgagatcggc gtgcagaccg
     2641 aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga tcgccgctct tcttccaaac
     2701 gcccaaccgg gatgctgacc aacagtaata tggaagagat gaccaagttg ctgggcgaac
     2761 gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt gatcttcaac tgggatagct
     2821 accgtagccg ggtaacaggt aaagagtatt aatccccgtt tctggcggta tactgcgaca
     2881 caattccacc ttgagtaatg atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg
     2941 ccgccagcgc attaatttcc accggggtgc atgctgcgtc ctggaaagat gcgctctcca
     3001 gcgccaccag cgaacttggc aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg
     3061 cgtcattaac taacttgctt agcagcggaa accaggcctt aagcgcagat aacatgaaca
     3121 atgccgcagg cattctgcaa tactgcgcga agcaaaagct ggcgtcggta accgatgccg
     3181 aaaacatcaa gaatcaggtg ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag
     3241 acaccaacta tctggacggc attcagggtt tgctgaaaac caaagatggt cagcaactca
     3301 atctggataa catcggaacg actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc
     3361 tggtgttaaa gcaggggctg aacttcattt cctgaaccgc gaaacgccgc gttttagctg
     3421 ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg ttttccccct
     3481 gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc ctaacaacat
     3541 cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt gtgcttctca
     3601 aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccgtt gtcagaacta
     3661 ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc gggacgtaac
     3721 acctggtggt ttgcagccac attaacggtg ctggggctat ttgtcgtttt aaatatcacc
     3781 ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct ctatacctta
     3841 accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat cggcattgtg
     3901 ctggggctga cagcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat
     3961 ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc ggtggacgcc
     4021 agcccggcat ttcgtcagat aacggaactg gtgaaatccc agtcacgcga tggcgacccg
     4081 gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa actcaacctg
     4141 gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc tttcccggat
     4201 ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca cacgcagcag
     4261 ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg cataccgctg
     4321 tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc gcagaacatc
     4381 tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca gggcgcgaat
     4441 ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg gcttcgacca cttatacggc
     4501 tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc gcaacgactg gggattctac
     4561 gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg ctcaggtcag
     4621 cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt tatctcgcgt
     4681 acctgtaacc gcaaaaaata tgattttgac ggtaagccga accagtcatt cagcgcggta
     4741 agttgcagcc aggagaatat cgcggcgttt atcaacaaaa tcaaagcgtc accgtggttt
     4801 aaagataccg tcatcgtcgt ctcttctgac catttagcga tgaacaacac ggcgtggaaa
     4861 taccttaata agcaggatcg caataacctg ttttttgtca ttcgtggcga caagccgcag
     4921 caagagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt gctggacatt
     4981 ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca gtcgatgtcg
     5041 gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccggatat catccgcctg
     5101 tggaaattcc ctaaagagat gaaagagttc actatcgacc agcagaaaaa catgattgcc
     5161 ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa gcgcgtagaa
     5221 ccgctgccgg aaagtgaata ctcagcaccg ctgcgtttcc agctggccga tttcgctcca
     5281 cgcgacaatt tcgtctgggt tgaccgttgc tacaagatgg cacaactttg ggctccggaa
     5341 ctggcactct ccaccgactg gtgtgtctcg caggggcagc ttggcggtca gcaaattgtc
     5401 cagcatgttg ataaaacaat gtggaagggc aaaacggcat ttaaagatac ggtcatcgac
     5461 atggcgcgtt acaaaagcaa tgtcgatacg ctgaaaattg ttgataacga tattcgctac
     5521 aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag aagaggtgaa acagtttagc
     5581 gggatttccc gtccggagtc gtggggccgc tggtccaacg ctcagctggg cgatgaagta
     5641 aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac cgccaaagca
     5701 tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga acaaactctt
     5761 gtgctgggca atgaagtaac caccaccaca ctgcatttcg ataacccaac cgatgccgac
     5821 acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat cctcggacac
     5881 tcgccgcgta agctcgggat cggcatggtg gaaattaaag ttgtggaacg tgaagggtaa
     5941 ttcctgactg atgtgtaggt cgcataaggt gcgacctaca caccttctcc ggcctacaaa
     6001 acatcattga gaaacagaaa aaaacagcgc ccaacacttc cccggacgct gcatggcaac
     6061 tttaataatc tcgccttatc agaaagtaat tacaccctta atcaactcac gattgttaat
     6121 cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat
     6181 gtcggcagtg attttccctt ctgccatcag tcgaccgact ttagcgaaat cttccggcgt
     6241 ggcgttgcgg ctgcccatca tcgtcgtttc ttttttatgg aattccggat cggaaaactg
     6301 caactcacct ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa tcagattcac
     6361 ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc actttctgcg ccagcgaacc
     6421 accaaactgc gcccgtagct gcgcgtcaaa atcttcggct gacgggtcca gtacaggtaa
     6481 ttccagacgc gttgccacat gttcacggcg cgctggactg gtatccgcca ccaccacctg
     6541 tgcgccatcg gctttagcga ttgccgccgc gcccagaccg attggccccg ccccgaccac
     6601 cagcacctgc tcgccgggag caatggctgc gcgacgcacc gcatgagcgc taatagcgaa
     6661 aggttcaatc aatgctgccg cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac
     6721 cggcacgctc aaatactcac taaaaccgcc atcctgatgc acgccaatga ctgaaatttt
     6781 ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat tgctgacagg caacataagg
     6841 gatcacagca acttgctgac catttttaag atcagcaata tttttaccca gcccaacaat
     6901 ctccccacat atttcatggc ctaaaacacg tggataacta aaaaatggtt gatttccacc
     6961 ccaggcatga atatcggtac cgcaaatccc gacagactta atttttatta atgcttcatt
     7021 gtcacccgga ataggtatct cgcgttgttt ccagactaat tctttcggct gctggcaaat
     7081 taaaacattc atcgtagaca tgattcggct ctccattttt tgttgcctta gatatcaatg
     7141 aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg aaaacacacg cataaaaatg
     7201 ttttaaatcg ggttttaatg gactcacaag gaggaaaata tgagtcgttc acaaaattta
     7261 cgccacaatg tgattaacca ggtcattgat gatatggcgc ggggtcatat cccttcccca
     7321 cttccatcgc aaagtgcgct ggcggaaatg tacaacatca gccgcacaac agtgcgtcat
     7381 attctcagcc acttacgcga atgcggcgtc ttaacgcagg ttggaaacga ctatgttatt
     7441 gcgcgtaaac ctgaccatga tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa
     7501 aacaaagtgt ttgagcaggc ttttttcacg atgataaacc agcgtcagtt acgccctggg
     7561 gaaacttttt ctgaactgca actggctcgg gccgcgggtg tcagcccggt ggttgtaaga
     7621 gaatatcttt taaaattcgg ccgttataat cttattcaaa gcgaaaaacg cggccagtgg
     7681 agcatgaagc aattcgatca atcctacgcc gagcagctgt ttgagctacg cgaaatgctc
     7741 gaaacacatt cacttcagca ctttctcaac ctgcctgatc atgatccccg ctggctgcag
     7801 gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc
     7861 atgttctcgc aacttgacag agattttcac tccttactgc tttcagctgc cgacaatatc
     7921 ttttttgatc aatcgcttga gatcatctca gtcatctttc attttcacta tcaatgggac
     7981 gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg agcatatgac cattctcagc
     8041 gctctgattt gccgcagtga ccttgatgcc acccttgcgc tccgtaacca tttgaattca
     8101 gctaaacaat caatgattcg atccattaat gagaacacgc gttatgctca ttaagtacgg
     8161 attaaaaaca gcaaactatt agcgggtgca cggttttatc aaaagataaa taccgggaaa
     8221 ttcccgctta cctatgctca caatcagtcg actaaaaatg tttttgtgag cggtagtaaa
     8281 gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt aatgcacaat tacgtaaaca
     8341 attaaaatta taaatacagg agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg
     8401 ttcttcattt actccatctt cgtcggcaga tattcccgtg ccaccagatg gattagttca
     8461 acgcagtacc cgaattaaac gcattcaaac caccgccatg ttgttattat tttttgcggc
     8521 ggtaatcaat tatctcgacc gcagttcgct gtcggtagca aatttaacga ttcgtgaaga
     8581 attgggatta agtgccaccg aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg
     8641 gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg
     8701 actggggatg ttcttctggt cactgttcca ggcaatgtct ggcatggtgc acaactttac
     8761 gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa gcgccgatga acccatgcgg
     8821 tgtaaaagtc attaacgact ggttcaacat caaagagcgc ggacgcccga tgggcttctt
     8881 caacgcagct tctaccattg gcgttgccgt aagcccaccg attctggcgg cgatgatgct
     8941 ggtgatgggc tggcgcggga tgtttattac cattggtgta ctggggattt ttctcgccat
     9001 cggctggtat atgctctatc gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc
     9061 ttatctcaat gcaggtagcg tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg
     9121 cagcctgttc cgtaaccgta caatgtgggg aatgatgctc ggattcagtg gcatcaacta
     9181 cactgcgtgg ctgtatctgg cctggcttcc tggttacctg caaacagcct ataacctgga
     9241 tttaaaaagc acagggttga tggcggctat ccctttcctg tttggggctg ccgggatgct
     9301 ggtcaacggt tacgttactg actggctggt caaaggggga atggctccga ttaaaagccg
     9361 taagatctgc attattgccg ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca
     9421 agcgacaaca tccatgacag cggttctgct gattggcatg gcactgttct gtattcactt
     9481 tgccggaaca tcctgctggg gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc
     9541 ggtgggcagt atccagaact ttgccagctt catctgcgcc tcttttgcgc cgatcattac
     9601 tggttttatt gttgatacca cccactcatt ccgtctggca ctaatcatct gcggttgcgt
     9661 caccgcagcg ggggcactgg cgtacatctt cctggttcgt cagccgatca acgacccacg
     9721 taaagattaa tctccctatg cccgataaca ttttgcttat cgggcatttt tatggcgatt
     9781 aaaacgtttc ccagttctcc ccgctatctg ccactgccat tttacgcggc gtagctggcg
     9841 tcacggtttt taccacagcc gatgtttcac gctgctgttg ctgctgttgc tgaatccgga
     9901 acactgccac agcttcggtc aggcgactgg cctgctcttc cagcgcggcg gcggcggcgg
     9961 cagattcttc caccagcgcg gcgttctgtt gagttacccg gtccatctca gcaaccgcta
    10021 agccaacctg atcgatacca cggctctgct catcagaagc agaagctatt tcgcccataa
    10081 tgtccgtcac gcgggtcaca gcactgacaa tctccgccat tgtttccccg gcgctttcga
    10141 ccagcgtaga gccaacatcc actttcccca ccgagtcttc aatcaggctt ttaatttcac
    10201 gagccgcctg ggcgctacgc tgggccagat tacgcacttc tcccgcaacc accgcaaaac
    10261 cgcgcccttg ctcacccgca cgcgcagcct caaccgccgc gttcaaagcc agaatattgg
    10321 tctggaaggc aatgccgtca attacgctga taatatcggc gattttctgc gaactggtgg
    10381 agatatcgcg catagtctgc accacgttat ccaccacttt gccgccgcgt tgcgccgttt
    10441 cagaagcact taacgccaga tggctggcct ggcgcgcatt ttcggcgttc tgcttcaccg
    10501 ttgcggtcag ttgctccatg ctggctgccg tctcttccag cgaagcggcc tgttgctcgg
    10561 tgcgcgaaga gagatcgtta ttgccggtgg cgatttcgct ggcaccgcta tagatggcat
    10621 tggccccgtt gcgcacatca ccgacggtac gcatcagctc tccctgcata tggcgcaaac
    10681 tctctgccag ttgccccatc tcattagagc catccacctc aatcggtttc accagatcgc
    10741 cgcctgcaat atgacgaatg ctgtcaatca ggcgattcat tggcgctacc agcgaggctt
    10801 taataccgaa ccagacggcg aagatgaccg ccagtacgac gatcatcacg cccaccagaa
    10861 tccacatagc ctggctgtag gaggcattgt tatcgctgac ggcgatatca tagagccgat
    10921 cgttttgctc catgtaagcc acatactgct tctcgaaacc gtcctgatat ccctgggtcg
    10981 gctgatcaaa gaactcgttg attttgcctg cacctaatag ttggatcagc tccgccagcg
    11041 cattgtgata aatatcgtaa ttacgtttga tctctgccgc tgcggcggtg ctctgacgcg
    11101 ggtcacgcgg caacgcttcg taatccgccc agtttttttc cgcctgtttc agcgaaatac
    11161 tggcactctg catcagctca gcaacggttg aaccgctacc aatattattc tgatccatca
    11221 tgtagcggat acccgcgcgg ttgagggtgt tacgcgtctg caacaacgcg acccagctgc
    11281 cattcagcgt ggattgctgc tggcgaatgg tttgtaaaac agtgaaattt tctttgtcat
    11341 tctttaaggc attaaagaac agaccgcctg atgtcagttg taaaaggcca aaaacagcca
    11401 aaaccagcag taagctggtc acgatcttga tacgttttaa catggtttct ctttcctgtg
    11461 gaccgatgca agattttcgg cctggaaagc aaaaacttta tgaaaaggtg taacccgcca
    11521 tccatggaaa aaccaggcgc tggttatcag atactaaaaa gttattcatt atcgcccggg
    11581 atggcttctt gtcgggaatt gcccagagca ataaattcct ctaacagatg ggttaactgt
    11641 tgcattttct cggcagtaaa ctgagcttca atctgccgat aggcttcttc aatctgtgtc
    11701 tgagcgcggt tatacagcgc ctgcccctct ttggttagcg agatatagag cttccgctga
    11761 tcattgatcg gctttaatcg caacactaaa ccgtcgcgtt ccatccgcgt aaggatcccg
    11821 gtcaggcttg ggcgcaaaat gcaggcgcga tacgccagat cgtgaaaatc cattgatggg
    11881 ctttccgcca gaatacgcac gatgcgccac tgttgctcag tcaaattatg ccgtttaacg
    11941 attgggcgaa aataactcat cgccgcttct cgcgcctgga gcaacgcaat ggttagagag
    12001 tcgtgcatta tctttcccct gaaaaattca aaatcattaa tatagaaaca gttaatcatc
    12061 acgttaccgg gactgcgctg cccctgcaat tcttcatttt atcaaaattg agcatgtttt
    12121 gt
//
LOCUS       DAZFFI010000135        12074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFFI010000135 DAZFFI010000000
VERSION     DAZFFI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.135"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..105
                     /locus_tag="ACHJ12_004244"
     CDS             <1..105
                     /locus_tag="ACHJ12_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO4991947.1"
                     /translation="TNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            374..2611
                     /gene="nrfB"
                     /locus_tag="ACHJ12_004245"
     CDS             374..2611
                     /gene="nrfB"
                     /locus_tag="ACHJ12_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4991948.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2598..5570
                     /gene="nfrA"
                     /locus_tag="ACHJ12_004246"
     CDS             2598..5570
                     /gene="nfrA"
                     /locus_tag="ACHJ12_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO4991949.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5571..6461
                     /gene="ybcH"
                     /locus_tag="ACHJ12_004247"
     CDS             5571..6461
                     /gene="ybcH"
                     /locus_tag="ACHJ12_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4991950.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6644..7405
                     /gene="envY"
                     /locus_tag="ACHJ12_004248"
     CDS             6644..7405
                     /gene="envY"
                     /locus_tag="ACHJ12_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4991951.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSICRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            7918..8871
                     /gene="ompT"
                     /locus_tag="ACHJ12_004249"
     CDS             7918..8871
                     /gene="ompT"
                     /locus_tag="ACHJ12_004249"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4991952.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9058..10542
                     /locus_tag="ACHJ12_004250"
     CDS             9058..10542
                     /locus_tag="ACHJ12_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4991953.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(10726..10938)
                     /locus_tag="ACHJ12_004251"
                     /pseudo
     CDS             complement(10726..10938)
                     /locus_tag="ACHJ12_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11088..11756
                     /locus_tag="ACHJ12_004252"
     CDS             11088..11756
                     /locus_tag="ACHJ12_004252"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4991954.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT         3287 a         2966 c         2826 g         2995 t
ORIGIN      
        1 acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc agccatggac
       61 agaaactatc tcgcgtcagt ccttgcgggg agcgggcttt cgtaatcttg ccctgcctgc
      121 tcacctcctc caggcacttc cactacatta tgacctgctc ggtgccaccg ctccttttca
      181 ctatgacgtt aacctcccct ccttgacacc atgcttttgt ctacattcta cgcaaacggc
      241 aacttttata accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa
      301 agctgtcaca attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat
      361 gtaaggtttt tgtgtggact ggcttcttga tgtttttgca acctggctct acggcttaaa
      421 agtaatcgcg ataacgttag cggtcatcat gttcatcagc gggctggacg atttttttat
      481 tgatgtcgtc tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc
      541 gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc
      601 ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga
      661 ctacgaaaac tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga
      721 tgttgacgaa gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg
      781 tcccaccagc aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg
      841 tagcgccaat ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc
      901 gatggaattg cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccggt
      961 gtatccgttc gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc
     1021 agagctgcat ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc
     1081 aggcgtcggc acctgtttca gccgccgcgc cgtgaccgct ctgttagctg acggtgacgg
     1141 tattgctttc gacgtgcaga gtctgactga agattacgac attggtttcc gcctgaaaga
     1201 gaaaggtatg acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga
     1261 gcagcgcaaa tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt
     1321 ccccgatacc ttttcgactg cagttcgaca aaaatctcgc tggatcatcg gcattgtttt
     1381 ccagggcttt aaaacccaca aatggacctc cagcctgacg ctgaactact ttctctggcg
     1441 cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgctcca
     1501 gcttttgctg ttgctggcgt atgaaagttt gtggcccaat gcctggcatt tcctttctat
     1561 ttttagcggc agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa
     1621 ccgcatcgtg cagcgggtga ttttcgtcac tggctactac ggtctgacgc aggggctgct
     1681 atctgtcctg cgtcttttct ggggcaacct gattaacttc atggccaact ggcgcgcgct
     1741 aaaacaggta cttcaacacg gcgatccacg tcgcgtcgcg tgggataaaa caacgcatga
     1801 cttccccagc gtgactggcg atacccgctc gttgcgcccg ttaggccaaa ttctgctgga
     1861 aaatcaggtc atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct
     1921 acgcctgggc ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc
     1981 gctggcagag caaaacggcg tggcgtggga atccatcgat gcctggcaga tcccgtcctc
     2041 gctgattgcc gaaatgccag cctccgtagc gctgcattat gcagtactgc cgctgcgtct
     2101 ggaaaatgat gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc
     2161 cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt
     2221 cacggggtta cgtcactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta
     2281 caatgcggtt cagcatcagt ggctcacgga acagcagacc ggtgaaatct ggcggcaata
     2341 tgtgccgcat cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc
     2401 agcaattaac gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt
     2461 ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga
     2521 attacaagtt tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc
     2581 gcaactggag tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt
     2641 tactgctgac gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag
     2701 aagagctggg gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc
     2761 tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc
     2821 agcaggtgcc ggataatatt ccgctgactt tataccttgc ggaagcctat cgccattttg
     2881 gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg
     2941 cccgacttga gcgcagtctg gcggctattc cggttgaagt gaaaagcgtt acgactgttg
     3001 aagaactgct tgcccagcaa aaagcgtgcg acgctgcgcc gaccctgcgt tgtcgcagtg
     3061 aagtcgggca gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg
     3121 atgcgacgtt tgctgcatcg ccagaaggaa aaacgctgcg aaccgatctg ctgcaacggg
     3181 caatctacct gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga
     3241 acacattaag cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg
     3301 acgatcggat cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta
     3361 cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcaatg
     3421 aaaataagcc gctgtttacc acggacgcac aagagaaaag ttggctctat ctgttatcta
     3481 aatacagcgc caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc
     3541 gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag
     3601 cgcaaaaact gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca
     3661 gcgtggcgac ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag
     3721 aaccggcaaa tcttacccgc ctggatcaac tgacctggca actgatgcag aacgagcagt
     3781 cacgcgaagc tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca
     3841 gccagacttt aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctggcaacgc
     3901 cggcgaaggt ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa
     3961 gtcagttgcc gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt
     4021 cgccttccta cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac
     4081 ccggtgtggc gctgtatgca tggcttcagg ccgaacaacg acaaccgaac gcctggcaac
     4141 atcgtgcggt agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct
     4201 ggcagaaaat cagtcttcac gacatgagca atgaggatct gcttgctgct gccaataccg
     4261 cccaggcggc aggaaatggt gcagctcgcg atcgctggct acaacaggca gaacaacgtg
     4321 gactgggaaa caatgccctc tactggtggc tgcatgcgca acgttacatt cctggtcagc
     4381 cggaactcgc actgagcgat ctcacgcgct caatcaatat tgcgccttct gccaacgctt
     4441 acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt cccggcggcg gtgagtgatt
     4501 tgcgcgccgc gctggaactg gaaccgaata atagcaacat ccaggcagcg cttggttacg
     4561 ccttgtggga tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag
     4621 ggcttccgga cgatccggca ctgatccgac aactggccta tgtgaaccag cgtctggatg
     4681 acatgcctgc gacgcagcat tacgcccggc tggtgattga tgacattgat aatcaggcgc
     4741 tgataacccc actgacccca gagcaaaatc agcaacgctt caatttccgc cgtttgcatg
     4801 aggaggtcgg tcgccgctgg acgttcagtt tcgattcttc tatcggcttg cgttccgggg
     4861 caatgagcac cgctaacaat aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct
     4921 acgggcaact ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc
     4981 tctcagttta tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga
     5041 aaaatccgat gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc
     5101 tcgccgtcga acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc
     5161 gcgccagcgc ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt
     5221 caggctggtt tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc
     5281 aggcgtggac ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta
     5341 ttgagcctta cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc
     5401 agcttggcgg agtcggggtc cgctggaata tctggaccgg cgagacgcac tacgacgcct
     5461 ggccgcacaa agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac
     5521 gtaacggaga gcgcaacaac gcgtttctca ccattggagt gcactggtaa atgcgtaagt
     5581 tcattttcgt attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta
     5641 ttatctggca accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga
     5701 tgagtcagtt acgtttgcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg
     5761 atgcattcac ccagccagaa cagcgcgcgt tattgtttaa gcgtgccgca gctgcgcaac
     5821 aggctggcct gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga
     5881 aacagtcgtc cgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc
     5941 aagccagatt atggagcgcc gcgcctggcg taacgccgga tggctggtac atcagcgcgg
     6001 aaattgacga cctgaactgg cgcagcgaag ctgcccgtca gcctttgcta acatggttaa
     6061 acaacgcgca gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt
     6121 tcgccggaaa catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaactg
     6181 gcgttaatgt ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg
     6241 aacgttatct acaggccagc gccgattgcc aaagtcccgc ccctgccagc ggcattgttt
     6301 atgaactgtt tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggatgcag
     6361 agatcgcctc gctgttagcg aaacgttcct cttgcggtaa agacactctc tatttctctc
     6421 tgcgctattt gcccgtcgcg cacggcattc tcgagtatta aatctcctcc aggtaagtcg
     6481 ggtacgacct ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa
     6541 aagcgtgcta aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc
     6601 atagatcctt tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag
     6661 tgaaccttgc gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc
     6721 tacgtttacc cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga
     6781 actctcaacg ttaaatcacg tattaatccc ccacatcaac cgtaacatca tcaacgatta
     6841 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
     6901 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
     6961 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
     7021 gtctaacttt cttgagcaat cgcggtttat agccctactg atgtatatct tacgcagcag
     7081 cgtccgcgac agcatctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
     7141 aattgtcgcc agttcgctat gtttaagccc cagcctactc aaaaagaaat taaaaaacga
     7201 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
     7261 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
     7321 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
     7381 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
     7441 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaagttcct
     7501 agaacgacat tttaagtcaa caaattaccg cgccatctct gcgctcacac gtcccactac
     7561 ctcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtccc
     7621 tcttttttgt actaaaaaac atagcaattg aggataaacc tcatgctatt ttcgcttata
     7681 tgcctttaaa ggcatggcgc ttaaatagat aaaaacacca caaaagcata aaaaaccaca
     7741 cagtaaaacc gcaacataaa acaataacag ataattaaac caaaaacaga tagcgcattg
     7801 tgataatcat tcaatactaa acaaaatata aacagtggag caatatgtga ttgactcatt
     7861 aagttagata taaaaaatac atattcaatc attaaaacga ttgaatggag aacttttatg
     7921 cgggcgaaac ttctgggaat agttctgaca acccctattg cgatcagctc ttttgcttct
     7981 accgagacta tatcgtttac tcctgacaac ataaatgcgg acattagtct tggaactctg
     8041 agcggaaaaa caaaagagcg cgtttatcta gctgaagaag gaggccgaaa agtcagtcaa
     8101 ctcgactgga aattcaataa cgctgcaatt attaaaggtg caattaattg ggatttgatg
     8161 ccccagatat ctattggggc tgcgggctgg actactctcg gcagccgagg tggcaatatg
     8221 gtcgatcagg actggatgga ttccagtaac cccggaacct ggacagatga aagtaggcac
     8281 ccggatacac aactcaatta tgccaacgaa tttgatctga atatcaaagg ctggctcctc
     8341 aacgaaccca attaccgcct gggactcatg gccggatatc aggaaagccg ttatagcttt
     8401 acagccagag gtggttccta tatctacagt tctgaggagg gattcagaga tgatatcggc
     8461 tccttcccga atggagaaag agcaatcggc tacaaacaac gttttaaaat gccctacatt
     8521 ggcttgactg gaagttatcg ttatgaagat tttgagctag gtggcacatt taaatacagc
     8581 ggctgggtgg aagcatctga taacgatgag cactatgacc caggaaaaag aatcacttat
     8641 cgcagtaaag tcaaagacca aaattactat tctgttgcag tcaatgcagg ttattacgta
     8701 acacctaacg caaaggttta tgttgaaggc gcatggaatc gggttacgaa taaaaaaggt
     8761 aatacttcac tttatgatca caatgataac acttcagact acagcaaaaa tggtgcaggc
     8821 atagaaaact ataacttcat cactactgct ggtcttaagt acacctttta acgaagttaa
     8881 ccagattttc tccccggtgg tattgtataa ccccggggaa tttgtttaca gttacaagta
     8941 ggcaatatcc actaattttg taagcattta acctatgtca taacgtgtaa tctcttaccg
     9001 caacacaaga cctcaatata ttcaaatctt atctttattc cgatattcag agacacaatg
     9061 cgaaaaataa ttactcattt caaagttgtt ttaacgttac ttctaccagt aaccgtatct
     9121 gcccagcaga tacagtggca atcctgtatg gccagtcaat tcaaccactg gtttggtgag
     9181 gaaaaaccgt ctcctgactt actatgtggt tatttgtctg ttccattaaa atatacagac
     9241 acaggcggag atgcttctta tgaaaaaaaa tcacaagtca aactagcgtt gacaaaattg
     9301 ccggcaaaaa gcaagcataa aggaagtatc ctgataataa gtggtggtcc cgggttacca
     9361 ggcataaatc cttatattaa ctttgactgg ccagtcacaa atcttcgtga gtcatgggat
     9421 attattggat ttgatcctcg aggcgtcgga cagtccactc cgacaataaa ctgccggcaa
     9481 tcagatacag agactcagga aaacataacc gaaaagcaac aagtattaaa taaaattaat
     9541 gcctgtatcc ataataccgg agccgaagtc attcgccata tcggctctaa cgaggctgta
     9601 tacgatattg atcgtattag gcaagccttg ggggataaac aactgacagc cgtggcgtat
     9661 tcgtatggaa ctcaaattgc agccttatat gcagaacgtt ttccctacaa cgtaagatct
     9721 atcgttcttg atggagtcgt cgatatcgat gacctggagg acaacttcac atggcaactc
     9781 aaacaggcac agagttatca ggaaacgttt gatcgctttg catcctggtg tgcgcgtaca
     9841 aaaagttgcc cgctttcttc agacagagat aaggcaataa ctcagttcca tgagctatta
     9901 tcaaaattac atcacaaacc tttattagac agtaagggag aaaatatatc ttcagatgaa
     9961 ctcatatcat taacaacaga ccttctgcta tggcgttcat catggccaac ccttgcaact
    10021 gccatacgcc agttctctca ggggattgtc agtaatgaaa ttgaaactgc gctcagtgct
    10081 ccgatagcct cagaagagtc aagcgatgct tcgggggtaa tcctctgtgt agatcagggg
    10141 gatgagcaat taacaccaga agagcgaaaa tcccgaaaag acgctcttgc gaatgccttc
    10201 ccggctatta actttgacaa tggacgttcc gattcacctg atttttgtga attatggcca
    10261 atacatagcg acctgaacaa aactcgcctg aaaaatactg ttctgccctc tggtttactg
    10321 tttgtagcac acaaatacga cccaacaacg ccctggatta atgcccgtaa gatggcagag
    10381 aaattttcca gcccgttact aacaataaat ggtgatgggc atacattagc tctcaccgga
    10441 gttaatttat gtgtagataa agcagttgta catcacctga tcactccaca aaaaatagaa
    10501 aatatatact gcccaggaaa ttctgaagca gaaatacaat aatttcaaaa tattccgcta
    10561 tttgctccca atgcaaaaca tatattgcat tgggagcata ttcatatttt tgttattatt
    10621 gtatgaaatc gtctcatgta cacggtcctt ttttcgccga aaactgatat tactttaatc
    10681 aaaagttgac gtgccagatt cgaagtaaat ttataaaata tcaaatcaat atacattttg
    10741 caccttcgag atagtaatga ctgttactgt aacatgagtt acaaacagct tcatacccag
    10801 agctactgat gtatcaactc gattcaacaa tacccgatat ttattccatg cctccagcaa
    10861 cgatctttct tcctccgttg cgatttccag atctacagca tcctgcagtg gcgcaatata
    10921 ctcactgaat tcctggatgt agaactgtgt agtgacggtc ttccagccat tcggctcctg
    10981 ctgtatcgaa gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct
    11041 tgtaattcat cgccataatt gatttaattc acaaataaaa ctataacatg gtgaaattaa
    11101 tgaaaaaaaa cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg
    11161 actggtgggt tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc
    11221 ttcttccaca ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg
    11281 ggttttacct tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag
    11341 ctagcctgaa cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattaacc
    11401 atgatgtttt tgaaccttat cccgcggcgt tacatggtca atttgattcc atttccatgt
    11461 tttaccttct tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg
    11521 cggcgcaggc cttaactgac gatggaactc tatacggagc cacaattctt ggcgatggag
    11581 ttgtgcacaa tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt
    11641 caaacacaaa agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg
    11701 ttaaaaccaa ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc
    11761 caaccgcagc acgttcttgc ataaggcgtg tctgcggttt ttcaactatt cagatacatc
    11821 actcccatca cattcattcc tccgcatcat aggcatatag gctatatcac cttgatattt
    11881 ttcttcttca gataaaaact gttatctatg tatactttta aacccaatcc gtgtagagtc
    11941 tctgcataag atagtttgca gttgccactt cagcttgcgc cataaaccgc ctgatttttg
    12001 ctgccacctg ttagcattcc tgtatacctg aaacgacaat gtttatctac gaactttaag
    12061 aacccccagg ataa
//
LOCUS       DAZFFI010000136        11770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFFI010000136 DAZFFI010000000
VERSION     DAZFFI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.136"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHJ12_004253"
     CDS             complement(37..318)
                     /gene="yehE"
                     /locus_tag="ACHJ12_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO4991955.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(581..1690)
                     /gene="apbC"
                     /locus_tag="ACHJ12_004254"
     CDS             complement(581..1690)
                     /gene="apbC"
                     /locus_tag="ACHJ12_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4991956.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1822..3855
                     /gene="metG"
                     /locus_tag="ACHJ12_004255"
     CDS             1822..3855
                     /gene="metG"
                     /locus_tag="ACHJ12_004255"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4991957.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3996..7790
                     /locus_tag="ACHJ12_004256"
     CDS             3996..7790
                     /locus_tag="ACHJ12_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO4991958.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7800..11432
                     /gene="yehI"
                     /locus_tag="ACHJ12_004257"
     CDS             7800..11432
                     /gene="yehI"
                     /locus_tag="ACHJ12_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4991959.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRFIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11493..>11770
                     /gene="yehK"
                     /locus_tag="ACHJ12_004258"
     CDS             11493..>11770
                     /gene="yehK"
                     /locus_tag="ACHJ12_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4991960.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIF"
BASE COUNT         3058 a         3008 c         2917 g         2787 t
ORIGIN      
        1 cggctttcta aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat
       61 tcgacgtttg ctatcgctac ctgcaacaac cacttctgtc gttacacctt tgacgggcga
      121 agaaaacaga tgacgggtgt caacattata cgttaactga ccacaatgct gttgtggttg
      181 actattaacc atagccatac tgcatgttgg gggactgatc ctgccattaa tcgttaatgt
      241 ggcagtctct gaagagaatg cgggagaagc caatccatag gcaagaaaaa taattcctga
      301 cagccaatac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg
      361 agtgcataac aaactatagc tgtacatcca ctacacagcc acgaaggatg ataatgaagc
      421 attgcctgta tgatcaatcg actttgtaga gtttcggacg aaggtccgca gaatattcgc
      481 agtattaaat aagtgttcat taatgcggca aaaactaata caccgcatca atgtaacatc
      541 tctgcggtgt atgaagaata accagaatgg ttaataggcg ttagaccgcg cggaaggaaa
      601 tctcgcctgg aatgacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct
      661 ggcggtagat agcggtaaat tcgctctccg gacggctgat aaccgttggc gtgcctttat
      721 cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgt gtgtggtatt
      781 tttcagccag tttctcagca ccaccggtac cgaaaatcgg ttcatggtga ccacagttac
      841 tgcaaatatg cacactcatg ttctcgacaa taccaagcac cggaacctcg actttctcga
      901 acatcacaat gccttttttc gcatcgatca acgcgatgtc ctgcggggtg gtgaccacga
      961 ctgcaccagt tactggaatg ttttgcgcca gcgtcaattg aatgtcaccg gtgcctggcg
     1021 gcatatcaag caccagatag tcgagatccg gccacaaggt ttcctgcaac atctgcatta
     1081 acgccttgct cgccatcggt ccacgccaca ccatcgcatt gtcgtcagtg accagataac
     1141 caattgaatt ggttgccagg ccatgagaca taatcggtgc catgtgggta ccgtccggcg
     1201 acgttggacg ctgattttct gcgcccagca tggtgggaat tgatgggccg tagatatcgg
     1261 cgtccagaat gccgactttc gccccttccg ccgccagtgc cagcgccagg tttaccgccg
     1321 tggaggattt acccacgccg cctttaccgg agctgaccgc aatgatgttc ttcacaccat
     1381 taatacccgg ctgattcttc acgcgtttta aggtggcgat attgtgcgac agtttccagt
     1441 cgatagcttt cgccccggtg atacgcagca gttcggcgct acattgctct tttaactctt
     1501 caaacgcgct atgccagacg aatggcataa ccagttccac atgcaacgtg tcgtccatcc
     1561 aggcgacatg gtgcaacgct ttcagcgtgg tgaggttatg cttcagggtt gggtgctgaa
     1621 aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt
     1681 gttcgttcat cccgactcct tttttgtata gataaaccat cagctgatag tttacctgaa
     1741 gaatatagag aagtacttac ttaacatttt cccatttggt actatctaac cccttttcac
     1801 tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca
     1861 ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat
     1921 gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat
     1981 gcccacggta caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg
     2041 attggcgaaa tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac
     2101 aactatcact cgacgcacag cgaagagaac cgccagttgt cagaacttat ctactctcgc
     2161 ctgaaagaga acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa
     2221 ggcatgttcc tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat
     2281 caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga attgatcgag
     2341 ccgaaatcgg tggtttctgg cgctacgccg gtaatgcgtg attctgaaca cttcttcttt
     2401 gatctgccct ctttcagcga aatgttgcag gcatggaccc gcagcggtgc gttgcaggag
     2461 caggtggcaa ataaaatgca ggagtggttt gaatccggcc tgcaacagtg ggatatctcc
     2521 cgcgacgcgc catactttgg ttttgaaatt ccgaacgcgc cgggcaaata tttctacgtc
     2581 tggctggacg caccgattgg ctacatgggt tctttcaaga atctgtgcga caagcgcggc
     2641 gacagcgtaa gcttcgatga atactggaag aaagactcca ccgccgagct gtaccacttc
     2701 atcggtaaag atattgttta cttccacagc ctgttctggc ctgccatgct ggaaggcagc
     2761 aacttccgca agccgaccaa cctgtttgtt catggctatg tgacggtgaa cggcgcaaag
     2821 atgtccaagt ctcgcggcac ctttattaaa gccagcacct ggctgaatca ttttgacgct
     2881 gacagcctgc gttactacta cactgcgaaa ctctcttcgc gcattgatga tatcgatctc
     2941 aacctggagg atttcgttca gcgtgtgaat gccgatatcg tgaacaaagt ggttaacctg
     3001 gcctctcgta atgcgggctt tatcaacaag cgttttgacg gcgtgctggc aagcgaattg
     3061 gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg
     3121 gaaagccgcg aatttggtaa agcagtgcgc gaaatcatgg cgctggctga tctggctaac
     3181 cgctatgtcg acgagcaggc tccgtgggtg gtggcaaaac aggaaggccg cgatgccgac
     3241 ctgcaggcaa tttgctcaat gggcataaac ctgttccgcg tgctgatgac ttacctgaag
     3301 ccggtactgc cgaaactgac cgagcgcgca gaagcattcc tcaatacaga actgacctgg
     3361 gatggtatcc agcaaccgct gctgggccac aaagtgaatc cgttcaaagc gctgtataac
     3421 cgcatcgata tgaagcaggt tgaagcactg gtggaagcgt ctaaagaaga agtgaaagca
     3481 gctgccgcgc cagttactgg cccactggca gatgatccga ttcaggaaac catcaccttt
     3541 gacgacttcg caaaagttga cctgcgcgtg gcgctgatcg aaaacgcaga gtttgttgaa
     3601 ggttctgaca aactgctgcg cctgacactg gatctcggcg gtgaaaaacg caatgtcttc
     3661 tccggtattc gttctgccta cccggatccg caggcactga tcgggcgtca caccattatg
     3721 gtggccaacc tggcaccgcg taaaatgcgc tttggtatct ccgaaggcat ggtaatggct
     3781 gctggtcctg gcgggaaaga tatcttcctg ttaagcccgg atgctggtgc taaaccaggc
     3841 catcaggtga aataatcccc ctttaaggcg ctgcatcgac agcgcctttt ctttataaat
     3901 tcctaaagtt gttttcttgc gattttgtct ctctctaacc cgcataaata ctggtagcat
     3961 ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc tatcaggacg
     4021 aaaaatcaca taaattctgg gcagttgagc aacagggaaa cgagttgcat atcagttggg
     4081 gaaaagttgg caccaaaggg caaagccaga taaaaagttt ttcagatgct gcggcagcgg
     4141 caaaagcgga gcttaagctg attgcggaga aggtgaagaa ggggtatgtg gagcaagcga
     4201 aggataattc tttgcaacct tcccaaacgg taacgggctc tctcaaggta gcggacttat
     4261 ccaccattat tcaggaacaa ccctctttcg tagcagaaac ccgtgcgcct gacaaaaata
     4321 cagatgctgt tttaccgtgg ctggcgaaag atattgctgt cgtttttccg cccgaagttg
     4381 tacacaccac gttaagtcat cgccgctttc ccggagttcc tgttcagcaa gcagacaaat
     4441 tgacccaatt acgtcgctta gcctgtagtg tgtcgcaacg ggataataaa acagccacat
     4501 ttgacttcag cgcctgttct ttagaatggc aaaacaccgt cgcccaggcg atcagtcaga
     4561 tcgacggcct gaaaacaaca cagctaccat caccagtaat ggctgtactc acggcacttg
     4621 aaatgaaatg cacaagatat aaagtgcgtg aggatgttat ggatcagatc gtccaggaag
     4681 gcggtctgga atatgctact gatgtaataa tacaccttca acagattgat attaaatggg
     4741 attatgcgaa taatgtcatt attattctgc cgtctggcat tgcacctgac tacttggagc
     4801 aatattccag atttgaatta cgcctacgta aacatttatc actggcggaa gagtctctct
     4861 ggcaaaaatg tgcacaaaaa cttattgccg caattccaca tattccagaa tggcggcaac
     4921 cattaattgc tttgttatta cccgaaaaac cagaaattgc acatgaaatt gcccagcgtc
     4981 tactggggca aaaaaaatta ccctcgcttg agtggttaaa aatagtggcg actgatgagc
     5041 acattcttgc ctcattagaa aaatatcacg agccatatgc catttttgat gattactatt
     5101 gtggtgcgat atggtcagcc accgtattac aggagcaagg tgttgcagcc ctgccccgat
     5161 ttgctcccta tgccgcaagt gactactgcg ccgatgtgtt gcgtcatatc aatcatccgt
     5221 tcgcattaac actgcttata cgtgtagccg ggcatactaa acgctgtcac gatcggatga
     5281 cgaaagcctg cgctgcgttc ccacatgcag caatggcggc actgacggaa cttcttgggc
     5341 aaaaagaaga gaacagttgg cgcattatgc taatgacaat gcttatctca caaccagcac
     5401 tggcagaaca ggtcattccc tggctctcga cacccgcagt tgccgtactg aaatcatgcc
     5461 agcaacaact gacacagccc tcaaaccatg ccagcgccga tctactgcca gccgtagtag
     5521 tctcccctcc ctggctttcg aaaaagaaaa aatcgccgat tccggtgctg gatttagcgc
     5581 cattaggcat tgagccaatc tgttatctga cagaagaaat cagtaatcaa cttttggcga
     5641 aatatatctg gtattcaaaa cacatcacgg ttagccatga agaaagtact accaacctgt
     5701 tggcaaggat gggttttcaa cgacggatcg ctggtacata tattaaagct cccgaagcgg
     5761 tagttgaggc atggctaaat gaagattatt caaccttact aagtgaattt aaggtgtttc
     5821 attcacctac cgggcattat tggcagttgg ggattttgac aacattgccg ctggagaaag
     5881 cagtaaaagc atggaatgcc cttaccctat ctccacatac cgataccgaa tacgccatgt
     5941 tacattttgg actcaaaggg ttacctgggt tagtaaactc acttgcacgc tatccacaag
     6001 aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc gtcgcccgtg
     6061 ccttcaacaa actgaaaacg ctacgcgaaa acgcccgtag ctggctgttg aaatacccgg
     6121 aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa gcacaggata
     6181 acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta ctgcaagaaa
     6241 tcgcccgacg ttataaccag ccggaagtaa ccgatgcggt gaacgctctg cttgcgctcg
     6301 atcccttaga taatcacccg acaaaaatcc ccactcttcc ggccttttat cagccatcgc
     6361 tctggacgcg cccggtatta aaagcaaatg cccaatcact gccagatagc gccctcctcc
     6421 acctcggtga aatgctccgc ttccctcagg aagaggctct gtatccggga ttattgcagg
     6481 tgaaagacgt ctgttccgcc gactcactgg cgggatttgc ctgggatctg tttaccgcct
     6541 ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttctcg
     6601 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
     6661 agcataaacg cgccaccgtt gggttggata ttctcgctgc tatcggtagt gatatcgccc
     6721 ttatgcagct taacggcatc gcccagaaac tgaaattcaa agcattacag gagcgggcaa
     6781 aagaaaaaat tgccgacatt gccgagagcc gcgaactcac ggtggcggag cttgaagatc
     6841 ggttagcacc ggatctcggt ctggatgata acggttcgct gctgctggat tttggcccac
     6901 ggcagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat gtttccggca
     6961 gccgcctgaa agacctgccc aaaccgaaca aaagcgatga tgaaacgcgg gcgaacgatg
     7021 cggttaaccg ctacaaattg ctgaaaaaag atgcgcgtac catcgccgcc cagcaggtag
     7081 caaggctgga atccgccatg tgcctgcgcc gccgctggtc gctggaaaac ttccagctct
     7141 tcctggttga gcatccgctg gttcgtcact taacccgccg tctgatttgg ggcgtttata
     7201 gcgccgaaaa ccagctactg gcttgctttc gcgtagcaga agataacagc tacagcaccg
     7261 ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcact cctcacgttc
     7321 tggaaatatc accaacggat gctgccgcct ttggtcagct ttttgccgac tacgaactgc
     7381 taccaccgtt ccgccagctc gaccgtaaca gctacgccct gacagaagcc gagcgcaatg
     7441 ccagtgaact gacccgctgg gcaggcagaa aatgcccgag cggtcgggtc atgggactgg
     7501 cgaataaagg ttgggtgcgt ggtgaaccgc aggatggtgg ctggatcggc tggatgatca
     7561 aacctctggg ccgctggtcg ttaatcatgg aaatcgatga aggctttgca gttggtatgt
     7621 cgccagccga acttagcgcc gagcaactct taagcaagct gtggctatgg gaaggcaaag
     7681 cagaaagcta tggctggggg agtaattcaa cacaggaagc gcagttctcc gtactcgatg
     7741 ccatcaccgc cagcgagctg attaacgata ttgaagccct gtttgaataa ggaaagagca
     7801 tggacaagga attaccgtgg ctggcggata acgcccaact ggaactgaaa tataaaaaag
     7861 gcaaaacacc actcagccat cgcaattggc cgggcgaacc agtgcctgtt atcactgaaa
     7921 gcatcattca gacactgggt gatgaattgc tacaaaaagc tgagaagaaa aaaaacattg
     7981 tctggcgtta tgagaatttt tcactggagt ggcagtccgc cattacgcag gccatcaact
     8041 tgatcggcga acacaaaccc tcaatcccgg cccggacaat ggcggcgcta gtctgtatcg
     8101 cgcaaaatga cagccaacag ttgctcgacg aaatcgtcca acaagaggga ctggaatatg
     8161 cgactgaggt ggtgattgca cgccagttta ttacacgatg ttatgagagt gatcctctgc
     8221 tagtgacatt gcagtatcag gacgaggatt atggctatgg ttatcgctca gaaacctata
     8281 acgaattcga tctccgactg cgtaagcatc tctctctggc agaggaaagc agctggcagc
     8341 gttgcgccga caaactcatt gccgcactgc caggaatacc caaaattcgc cgtcctttta
     8401 ttgcactact cctcccggaa aaacctgaaa tagccaatga actcgccagt cttgaaagct
     8461 cccggtcaag tctccattca aaggagtggt taaaggtcat tgctactgat aatacggcgg
     8521 taaaaaaact cgaacgatgc tggggcctgg acgtatttag cgatcgagaa gccagctaca
     8581 tgtcgcatga aaaccacttc ggctacgcgg cctgcgccgc ccttttgcgc gaacaaggac
     8641 tggcagccgt tccgcgcctc gcgatatatg cccataaaga agattgcggc agtctgctgg
     8701 tacaaattaa ccatccgcaa gtcatccgca ccttgctgtt agttgctgat aaaaacaaac
     8761 ccagcctgca acgtgtggct aaatacagta aaaacttccc ccatgcgaca ctcgccgcac
     8821 tggcagaact gctggcgtta aaagaaccac cagcccgccc aggttatcca atcatcgaag
     8881 acaaaaagct gcctgcacag caaaaagcac gcgatgaata ctggcgtacg ctgttacaaa
     8941 cgctgatagc atcgcagcca caactagcag aaagggtgat gccgtggtta agtactcaag
     9001 ccagggcagt gctgaatagt tatttatcgg caccgccaaa accggttatt gatagcaccg
     9061 ataacagcag tctgcctgaa atgctcgttt caccaccgtg gcgtagtaag aaaaaaatga
     9121 cagctccacg tcttgatttg gcaccgctcg aattaactcc gcaaatttac tggcaaccag
     9181 gcgaacaaga gaggcttgcc gccactgagt ctgcccgtta tttcagcacg gaatctcttg
     9241 cggaacgcat ggaacaaaaa agtggacgag ttgtattaca ggaactgggt tttggggatg
     9301 atgtatggct gtttctgaat tatatactcc ccggaaaact ggatgctgca cgcaattcac
     9361 tcattgttca gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact
     9421 ccccgcaagc acaattagca gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta
     9481 acatatggga aaatgacaac ttctcacgtt atcgtccgga aaagagtgtc tggaacctgt
     9541 atttattggc acagttgccg cgtgagatgg ctttgacctt ctggctgcgt atcattgaga
     9601 aaaagcatct gttcgcgggt gaggactatt ttctcagtat cctcggattg gatgcgctac
     9661 caggtctgct gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt
     9721 ttggcgcaac agaactggcg ctgcctgttg cccgcgtctg gcgacgtttc gcggcgcagc
     9781 gtgatctggc tcgccagtgg attttacaat ggccggaaca tacggctagt gcacttatcc
     9841 ctcttgtctt taccaaaccc agcgataata gcgaagccgc attacttgcc ctgcgtttac
     9901 tgtacgaaca gggacatggc gaattgctac aaaccgtggc aaaccgctgg cagcgtacag
     9961 atgtatggtc tgccctggag cagttgctta aacagggtcc aatggacatt tacccggcac
    10021 gcattccaaa agcccctgat ttctggcatc cggcaatgtg gtccaggcct cgacttatca
    10081 ctaataatca gcctgttacc ggtgacgctc tggaaattat cggcgaaatg ctgcgcttta
    10141 cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa
    10201 cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt gcccccgcaa
    10261 aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg gcacgggatc
    10321 tgacgacaca gatcctcgcc tggccacaag aaggcaaatc tgcccgtgct gtcagcggcc
    10381 tgaacatcct taccctgatg aataatgata tggcgctgat acagctgcat catatatcgc
    10441 aacgggctaa atcccgcccc ttacgtgata acgcggcgga atttcttcag gtggtcgcag
    10501 aaaatcgcgg gctaagccag gaagagctag cggacagatt agtcccaacc ctgggccttg
    10561 atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt cgcttcgatg
    10621 aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagc gttccccggt
    10681 tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta aaagggctaa
    10741 aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct gccctacgta
    10801 ccacccgacg ctggtcgctg gcagattttc atactctgtt tgttaatcat ccctttaccc
    10861 gcctggttac ccagcgattc atatggggcg tgtatccggc aaatgaaccg cgtcgtttac
    10921 tcaacgcctt tcgtgtggcc gcagaggggg agttctgcaa tgcgcaggat gagccaattg
    10981 acctgcctgc ggatgctctg attggcattg cccacccgtt agaaatgaca gcagaaatgc
    11041 gcagtgaatt tgcccagctt tttgccgatt acgaaattat accgccactt cgccagttga
    11101 cacggcgcac ggtgctgctc acgcctgacg agtcagccag taatagtttg aatcgctggg
    11161 aaggtaaatc tgctaccgtt gggcaactta tgggaatgcg atacaaaggc tgggagtcag
    11221 gctatgagga cgcatttgtc tatgacctgg gtgagtaccg gctggtcctt aagttttcac
    11281 ccggttttaa ccactacaat gttgatagca aagcgctaat gagcttccgt tctcttcgag
    11341 tgtacagtga caataaatcc gtcacttttg ccgaacttga tgtgtttgat ttgagtgagg
    11401 cgttaagcgc acctgacgtc attttccatt aaaacacagc gggcagtgat gcaactgccc
    11461 gttatcaaca cgacttacca gcggggaaag cgatgatcgt gcaaaaagag ctggttgcta
    11521 tttacgatta tgaggtccct gtacctgaag atccgttttc cttcagactt gagatccata
    11581 aatgctctga attatttaca ggttccgtct atcgactgga gcgattccgg ctacgtccaa
    11641 catttcatca acgtgatcga gaagatgctg acccgctaat aaatgatgcg ttgatttata
    11701 taagagatga gtgtattgat gagcggaaat tacgaggtga atcacctgaa actgtaatag
    11761 caatttttaa
//
LOCUS       DAZFFI010000137        11497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFFI010000137 DAZFFI010000000
VERSION     DAZFFI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.137"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(591..782)
                     /locus_tag="ACHJ12_004259"
     CDS             complement(591..782)
                     /locus_tag="ACHJ12_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991961.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(782..1201)
                     /locus_tag="ACHJ12_004260"
     CDS             complement(782..1201)
                     /locus_tag="ACHJ12_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4991962.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRLMRQALDGENG"
     gene            complement(1248..1673)
                     /locus_tag="ACHJ12_004261"
     CDS             complement(1248..1673)
                     /locus_tag="ACHJ12_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4991963.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(2088..2864)
                     /locus_tag="ACHJ12_004262"
     CDS             complement(2088..2864)
                     /locus_tag="ACHJ12_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991964.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRCVSYQVADDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDYPCWLDEDDENLPVVLNAILNRGARFSSVEMYLVSECVEHILASGLVCDV
                     LRIPDEPSRRWFDREILREVVLEARDEIRSMADALAKIRK"
     gene            complement(2910..3428)
                     /locus_tag="ACHJ12_004263"
     CDS             complement(2910..3428)
                     /locus_tag="ACHJ12_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4991965.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVINQA
                     RTLLKNNRHEND"
     gene            complement(3358..3579)
                     /locus_tag="ACHJ12_004264"
     CDS             complement(3358..3579)
                     /locus_tag="ACHJ12_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991966.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(3580..4179)
                     /locus_tag="ACHJ12_004265"
                     /pseudo
     CDS             complement(3580..4179)
                     /locus_tag="ACHJ12_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            4801..6501
                     /gene="ltrA"
                     /locus_tag="ACHJ12_004266"
     CDS             4801..6501
                     /gene="ltrA"
                     /locus_tag="ACHJ12_004266"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO4991967.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKILPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            complement(6583..6654)
                     /locus_tag="ACHJ12_004267"
                     /pseudo
     CDS             complement(6583..6654)
                     /locus_tag="ACHJ12_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(6731..7042)
                     /locus_tag="ACHJ12_004268"
     CDS             complement(6731..7042)
                     /locus_tag="ACHJ12_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991968.1"
                     /translation="MKYDGDGWATARFFSDKGCRRAPLFTAPADAARQERCLWSVSRV
                     RRARDGRFYRSRLAPVTVYASLSPFSDERPSSRFRGIALPSNRRRLRYSTVGLTRYRT
                     R"
     gene            complement(7039..7941)
                     /locus_tag="ACHJ12_004269"
     CDS             complement(7039..7941)
                     /locus_tag="ACHJ12_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4991969.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRCATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            8381..9340
                     /locus_tag="ACHJ12_004270"
     CDS             8381..9340
                     /locus_tag="ACHJ12_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4991970.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            9340..9735
                     /locus_tag="ACHJ12_004271"
     CDS             9340..9735
                     /locus_tag="ACHJ12_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4991971.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDSLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDINVLKNIIE
                     ASSENKSESIASKDDFEEQTRRNLSMLKK"
     gene            complement(9850..10182)
                     /gene="pemK-mt"
                     /locus_tag="ACHJ12_004272"
     CDS             complement(9850..10182)
                     /gene="pemK-mt"
                     /locus_tag="ACHJ12_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFO4991972.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(10184..10441)
                     /gene="pemI"
                     /locus_tag="ACHJ12_004273"
     CDS             complement(10184..10441)
                     /gene="pemI"
                     /locus_tag="ACHJ12_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFO4991973.1"
                     /translation="MHTTRLKKVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(10534..11187)
                     /locus_tag="ACHJ12_004274"
     CDS             complement(10534..11187)
                     /locus_tag="ACHJ12_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFO4991974.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGPGKNYLF
                     FSHGAVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYIGVFLLGKVLAL
                     PAEPFMTKLFADKSIPDVSLTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYKNLLTLAELFLVGVITSAARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT         3049 a         3165 c         2890 g         2393 t
ORIGIN      
        1 caggttgtgg ctgtatcagc ccccggcgac aggggcttaa acaggtgaat ggcaccggac
       61 agaacaatgg gcagggaccc gccgcaacga cgtgaagcgc ggtttgcgaa acggcgttgc
      121 cccttacatc ccggcgtctt tttgccggga tgccaggagc gcaggccacg gcgcgacaag
      181 ggtggcgcga ccggaacggt cgctgcaagc cgggggtttt cacgcatcgg accgtagcga
      241 cagcccgaaa ccgcgccagc gggttcggcg gagactggcg ggtgcgccag cagctgcctg
      301 tcggagtcgg gcctgacgac cggatgccgc cacgcagccg ggagccgaag gtgcgcccgg
      361 cagacaggac aacactgcag tagtcagaat cagagcagca gagaaaagaa caacaacaac
      421 atcaggaaca gttccataac ataagaaaga aacactcctg agtcagacac atcacaaacg
      481 ggaaggggaa cgggcgacgc gccagcgtgg cccgcttccc ttccggcagg cgccagcctg
      541 ccaggggttt tcgcgcgggc tgacaggcgg cctccaccgc ctgccctgta tcacctgtgc
      601 ctgctgccct gaaggatgta gtgaccaccg aggaaaaccg cctgctgatt cgccacaaaa
      661 tcacagattt catcaacaat ctgcgcagtg gtgagcggtt tctgccccat ttcacgacgc
      721 cgcgcattaa tcacggccct gtaattacgc agaatttcgc gggtttccgt gctgatattc
      781 attacccgtt ctccccgtcc agcgcctgac gcatcaaccg cataatctca cgttcttccc
      841 tgacaaatgc gtcaccgtcg cccccgttct cgctccctac agcatggagg cgataaattt
      901 cactaccagc catcaccatc actttttcca gcagcaccgc agggactgtt acaagacggt
      961 ttacgcgctt ttcctccatc agttcacgaa caacgtcgtg aacaatgctg acatcagcac
     1021 catgctcaaa ggcatcgtcc agccgctgca tgacggtttc cagctcatcg tctgtcagac
     1081 tccagtcctg ggctatgtga cgcacatccc cacgggtcag caccacggca aaaacgcatt
     1141 cactgtccgg cacgtctgta tccagcactt cgcggataat ttctttaaca gtacagtaca
     1201 tgttctcgtc tcctttacag gggcggcatc gccgccccgt ccgttaatca gtccagtgct
     1261 gcgtaaatcg cgttgcattc ggggtgaaat tcgatgtggc tccacaactg cgcatcgcgc
     1321 atccggtaca ggtgtgtcag tcccgcatca ccgctgtcgt ggtacagcca caactgtcgg
     1381 ttaatcacaa gggaagtcag aatgatacct gcggcatcag cactcacagt gcggtcaaac
     1441 cagttttcac cgttgaccag atgaaaacgg tcgccatccg gcatcatgta accgccaccg
     1501 tccggcaggc ggatgaaatg ccagaagcct ccctgatatt ccggcatcat tttttgtgcc
     1561 agcgcgtaca catacgcctc ggcatacaga aaatcgttgc caaacaggta aggcagaaaa
     1621 gacagacggt cgcactcttc aacgttcaga gtgacaggtt tcgcatactg catgggattt
     1681 cctccgttgt tcacatattc cgcgacaccg ggcggtgccg tgcctccatc tttgccgggc
     1741 ctgcagggtt gcaagggcgc aagggaagcc gcagcctgac gcacacggct gacgggagtt
     1801 ggagggtgag accgcgaaaa ctgagcgggt gaacgaccgg aaaatcacgg cagacgggaa
     1861 ggagggaccg ggtgaccgca gcggttcacc ttaccttgca acccgcccgc agaagcccgg
     1921 ctacgatgga ggcacggcac agcccgcaca cgacacggcg gccacataac tgaaaaccca
     1981 caaggccatg ccctgcatgg ccgggtaaag cggagcctgc ggcgacatcc ttttgctctt
     2041 tccggggacc ggctggaaaa ggcggagatt tttccccgcc agcgtcatca tttccggatt
     2101 ttcgccagtg catccgccat gctgcggatt tcatcccgtg cctccagaac aacctcccgc
     2161 agaatctccc tgtcaaacca gcgacgggac ggttcatccg gtatacgcag cacatcacag
     2221 accagaccac tggccaggat atgctccaca cactcactga ccaggtacat ttccacagaa
     2281 gaaaaacggg cgccacggtt cagaatggca ttgagcacca cgggcagatt ttcgtcatct
     2341 tcatcaagcc agcaggggta atcgctttcc ttcacaacca gacgaaagcg gcgcgtctgc
     2401 cgggtcagca acgtctgacc ggtggtggtc agcgccgccc ggatacgccg cgcatcgtct
     2461 gccacctggt aactgacaca gcgtactgca tcgcgcaccg catcaagaac tgccctgatt
     2521 tcgtcttcgg tcagccattc tccggcatac gggaaaaaca cctgtgttcc gtctgccgga
     2581 agtaccggca gcgactgcca ctgactgccg gacagtacca gttccgcccc ggttccggtc
     2641 agtttctgaa tggcggaggc caggagcggc ggcagtacgc cgccgtgacg gttgatgagt
     2701 aacgtcgcca gccggggcgc atccagcaga acataaatgc tgatccagcc cggatacttc
     2761 gggttccgct ggtcaacatc cgtctcttcc ggccagccct gcgccagcag caggttgcgg
     2821 gcgtgacgac aaatcagtgc gttcagcgtt gtgttttcgt tcatgattaa tcgttctccc
     2881 tgaatcgccg cccggaggcg gcgataacat cagtcgtttt cgtgtctgtt gtttttcagc
     2941 agggtgcggg cctgattaat caccgcctga cggcgggtga cgcattccgg gacggcgagg
     3001 ccatgatccc gggcggccca ttcacgtttc cacaatgcct gttcagcggt ctgaatcagg
     3061 gacgccagca gttcagcagg gacggtcacc tggcggtttt cgctcacgtt attgatgatc
     3121 tccatcgcca cgccggaaga aataccggat tcagtccgct ggtcttccgg gatgtcctcc
     3181 aggcgcgcca ggacggttct gatttcatga tcggacaggg aaatatccat tccgtcagca
     3241 agggcctgaa tctcttccgg agaccagata accagcatca gcggcgtgtc tcccggatac
     3301 tttgctgcca gctcccggca gagcgtttcg catgttccgt acattctcgt ctccttctca
     3361 atctttgaag caggaatcca gttccagaat ttcggtatac gcctccataa acaggcgatt
     3421 ggcgcgatac agcgtctggc gtgtcaggcg ttcaacaagc acatcggaat caaagcgaca
     3481 gcgatgctcc atatcgttca gcctggcccg aaggtcacac atggtgttgg ccacctccgc
     3541 cacgtcggcg cgaagttttt cgtgtccggc atagttcatt tacgcagcct ccggttcaaa
     3601 ccagttatca ttcgcggccc cctgctgcat ggcggccagt cgttgctgtc cggcacggtg
     3661 atactgttca agcagctcga taccgatata ccggcgtccg gactggaggg cggccacgca
     3721 ggttgagccg ctgcctgcaa acgggtccag cacaatcgcg ttcggatgtg tgaagctctc
     3781 aatcagtggt tgcaggctgg taacgggctt ttccgtcggg tgatgacgat tgcctgaata
     3841 tttccagccc agcacgtcgg gtaatggctt ctgaggcagc gccggacggc cttttgccag
     3901 gatataggcg cattcgtgac ggtacgcgac atacgcagat ttggatgtgt agcttttggt
     3961 gaacacaagg tgccctacaa cgctgaatcc ggcgcgtttc caggctgcca taaagcggtc
     4021 aatacggttc cagccataga aactgaccat cagcgcatct tttttgagta cgcggtacat
     4081 ctcattactg gcaggttgca gccagtcatc gttaacgtca ccggcaatgg tgcgcccgga
     4141 acggtcacga aaaccgacga gatatggtgg gtcggttaag gtgcgacgtg aaagtcgcac
     4201 agtttgttgt tttaacttca tgtaaaggaa gtagtaaaac caggcgttcc gtcgcctgta
     4261 gcctaccgaa gcatgcagag cgggtaactg ttcggtatga agccttcggg acaacgtagc
     4321 ccggttttca caccatgtgg aatccgcgag gaggaagcat gtctgccagt gtcagggcgg
     4381 atggggcgca aggctggatg gcagggttaa cacatgtgaa ctgctgataa acatcgtcaa
     4441 ccctgaacgg gccaaaggca ctgacaggcc cgacccaaaa tgggaacgta gctggacctt
     4501 cctcttttga ctggggaggc acggacatca aagctaccgg tggataggca gaacctaacc
     4561 catccctgca aactgtgcgg aacacggtaa gcccgtatca ctgccctcag ggcaggctaa
     4621 ccgcgaggaa agctgtcagt gatgcgggta caggagtgcg gagaaagcga atgccatcct
     4681 gtaatgggat ggacaggagt tgagctgttt cagcaacatc attccacgcg aaagcgggca
     4741 gacttccgca tggtctttca acacgagata acctgaatac ctttgaagga gggtaagcag
     4801 atgactgcga aaaacataaa cgcgggtgca cccaccgaca tcgtgactca ctggcacaat
     4861 atcaactggg ccaagtgccg tcgcgaggtc agaaggctgc aggcgcgtat cgtgaaggca
     4921 atacaggagg agaaagcggg caaggtaaaa gccttgcaat ggctactcac ccactcgttc
     4981 agcgccagag caatggcagt taaacgagtg acagataaca gagggaagag aacgccgggt
     5041 gttgatggca aaacatggtc aaaacccggc agcaagatga aggctatata cacgctgaaa
     5101 agaaggggtt acaaacctct tccgctacgg cgtatatata tcccaaaatc taacggcaaa
     5161 aagcgaccat tagggatccc gacaatgaaa gacagagcca tgcaggcact ctatctgatg
     5221 gcgctggaac ctgttgcaga aacaacggct gacccaaatt cgtttgggtt caggccctgt
     5281 cgctcaacag ccgatgccat tgagcaatgt ttcacaacgt tgcacagggc tgatcgggca
     5341 caatggattc tggaagccga cattcgcagt tgttttgatg agatttcaca tgaatggcta
     5401 atcgccaata tcccaacgga tacggcgatc ctgaaacgat ggctgaaagc cggatatatt
     5461 gatttgggga aattataccc aaccagtgca ggaactccac agggcggaat aatatctcca
     5521 acgctggcaa acatggttct ggatggtctg cagcctctgt tgaagaaaac cttctatcga
     5581 ggcggactga atccagaaaa gatcaacata atccgttatg cggacgattt tgtgatcacg
     5641 gggataagcc acgacacgct gtcagaaaag gttcttccct tgttagaaaa ctttctggca
     5701 gaaagagggc ttaccctctc accagaaaag acacgaatca cacacatcag tgatggattc
     5761 gactttctgg gtatgaacat ccgcaaatat aacgggaaac tgttgattaa accttcaccc
     5821 caaaaggtaa aggaattcct gaaaagggtc cgccagacga taaaagacaa tccgacagtc
     5881 agccagagca cactgatatg gcatctcaac ccagtcctca aaggctgggc cagatactat
     5941 cgtcatgttg tttcaaaaaa gatctttcag aaagtcagtc atgaaatatg gaaagcacta
     6001 tggcgctggg catgtcgacg acacccaaag aaaggtacag gctggataag aaagaaatac
     6061 tttcgttcag ttaacttccg acactgggta ttcggcacag aaacagggaa gatcctgcca
     6121 tcaggcaaac gtgagttact ggcgctatat gaaatctcgg atatgccagt caaaagacac
     6181 agacaaataa aaagcactgc taaccctttt gatcccagct acgaatcgta ctttgaagca
     6241 cggctaaaag ctacgatgct ggaatccata cagggttact ctcgcctaag aaatctgtgg
     6301 ctccgacagg gaggtcagtg ccccgtctgc cacctgccag tgaacgccac aacaggctgg
     6361 caacttcgac acattgtgcc gaaatccgaa gggggactgg ataacatagg taatctggcc
     6421 atgttacatc cggcatgtca tcacactgca aaacatcagg gaatatctgt tatgaagccg
     6481 gctctaatgt ggagttattg aaaggcttga gccgtatgcc gggaaactgg cacgtacggt
     6541 tcttaggggg cggctgcaca gtaatgtgca gctgctaccc gacatgaaat caatggcgtt
     6601 atcaggaatc gtggccataa cacgcacgca gtcacccagg ataaaacggg acataacagc
     6661 actcctcctc ttacagaggg caccgggagg gcgcccctgt ggcgcccgtt ccccgctgcc
     6721 tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc
     6781 gtctgtttga cggcaaggct atgccgcgaa agcggcttga agggcgttca tcagaaaagg
     6841 gcgacaggct ggcgtacact gtcaccggtg ccagccggga gcggtaaaag cgcccgtccc
     6901 gtgcccgccg gacgcgggac actgaccaca ggcagcgttc ctgccgcgcc gcgtcagccg
     6961 gcgcagtaaa aagaggagca cggcgacatc ctttgtcgga aaagaaacgg gcggttgccc
     7021 acccgtctcc gtcatacttc atccgccata atgcgccaca ttgtcgacta tccacgcagg
     7081 tccggtgact tccggcagct catcgtcatc tgtcggggaa gaccattcca gctcccccca
     7141 cagcacatca acaagctcgc cacgctcata tcgctgccag ccacagaaat tgcagccctg
     7201 ttcggcatac cagtgctcca gtgtgcagct gaaacggcgg cttaattctg caataacgtc
     7261 gctttccggc tgacaccacg gcgtgtcgaa atcaacctga atgaagttgt ctccctgact
     7321 gctgatattc aggtcatacc cacaaggcca cttcacgcca tactgttcgg tataccagtg
     7381 atatgcagaa ggaacaccgt tcagaacacc accgttaaag ccattcactt caacatcaag
     7441 gcgggtgggc agaaccgtca gcaggtcaaa cggcatggtt ttttccggca gtacgttgtc
     7501 acacagacgg ttccaccaca ccgatacatc ttcgttactc cagaagccac accagtcacc
     7561 tctttttgcg gtgaaaagcg cggtgatggt atccctgaca tcgtccggta atccttccca
     7621 gcgacgctgg ccagtaccgc tctgcagcca gagttcatgc agcatcaggc agttttgttc
     7681 atccagcagt acaccgtcct gcaggtgtgt cagccagcgg gtgaacgcga tattctccgg
     7741 cgatacaacg ccacgcccgg cagccgtcag tccggggcac ggttcaaacc acacatcttc
     7801 agtggtctgc agaagtccgg cacttccggc cagaaacagc tgaatacctt catttgtggc
     7861 gcagcgataa agcggtgtga ctgcaccgct ggccagtcgt ttaatctcag caatctgtgc
     7921 cggttcacca gaaaaatgca tacgatttga gcaccagttt ggcatgggaa ttctcctctc
     7981 acatgcggcc tccatcaggc cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag
     8041 gtgcggcccc gaagccgcag cgcgtggagg aggaacgacg aaacgtgacc gggtgaggag
     8101 gccgacgaca gcgacacact tgcatcggat gcagcccggt taacgtgccg gcacggcctg
     8161 gttaagtagt ttccgtctca gttcgtaacg caaaatgaga aaccaataga gactcaaatt
     8221 gaaccacaat aagaacacaa agatactcta aaagagccac accgcttgac acacacccaa
     8281 aatgagacac aataaggaac aacaaaaaac tcaaaaagat gcatcaacat gaatgatgct
     8341 caatttgaga caaacacaaa ctcaaaacga ggcaaaaaaa atgctaaaag tatcatgcga
     8401 tgatggttca actaacgtaa aactggcctg gcttgaagat ggcgaggtaa gaaccagtct
     8461 gtcaggaaac agctttaaag aaggctggaa cccgggactg tttaatgccg gcaaagttta
     8521 caattacgtc gtggatggaa aaaaatacac ctatgatttg ggcagcactg ctgtcatagg
     8581 tacaacacat gtcagttatc aatacagcac caccaatttg cttgccatac atcatgcact
     8641 gctgacatcc ggccttcagc cacaggatgt agaactgaca gtgacattac ctgtaaccga
     8701 attctttgat aatgacaatc aaccaaatga agaaagaata gaaagaaaaa aggcaaatgt
     8761 tctccgtgag atatctctga ataaagggga aacatttaaa attaaaaaag ttaatgtaat
     8821 gcctgaatct ttgccagctg catttgaatc actaaaaaaa gataaagtaa acaaacttga
     8881 acgcagcctg attatcgatc ttggaggaac cactctggat tgtggtttaa ttctcggtgc
     8941 atttgaaggc atttctgaaa tccgtggcta ttcagaaatc ggaacatccc gcataactca
     9001 taccgtcatg aatgcactaa caaaagcctc cacaccctgc aattatttta ttgctgatga
     9061 gttaataaaa aacaggcatg ataatgaata tctccagaca ttaataaatg atgtagctga
     9121 aataaaaaat attactcatg taatagacag tgaggtgaaa tctctggcag aaagcatcag
     9181 gcaggaaatt tcaacattct caggaatgaa ccgaatctac ctcacaggcg ggggagctga
     9241 gcttatttac cctcacataa agcaatattt cccgaatctg aaagtgaaca aggtagatga
     9301 acctcagttt gcattggtga aagcaatggt gcacgcgtaa tggaaagcag tgatccgaaa
     9361 aaaagaaaga aagttgtggc atacctgcac ccgacacttt acccgcagga cagcctgaca
     9421 caacaaacaa tagactctct cccgatacaa atgcgaggag acttctacag acaatctctt
     9481 atttgcggag cagctcttta ttctgttgac ccccgactgt taacgctaat cagtggtttt
     9541 ttcagtgaga aaatcactgc agaaaatctg gtaaaactta tagagcaaac aacaggatac
     9601 acatccacat caattgatat aaatgtgtta aaaaacatta tagaggcatc ttcagaaaac
     9661 aaatcagaga gcatcgcttc aaaagatgat ttcgaagaac aaaccagacg caatctgtct
     9721 atgttaaaaa aataataaaa gaagagcagg ttcagttatt acctgctctt cttttataat
     9781 gagggaccag cagcggtttt agtgccctaa atcgaacgtt ttcgtccggt tgcccctcaa
     9841 accccatgtt caagttagaa tagtggacag acggccaaga acttcgttca tgatagtctc
     9901 tggaacccgt tcgagtcgtt ttccgccccg ggctttcata tcaattgtcc ggggttgatc
     9961 gcaacgcaca acacctgtgg tacgtatgcc aacaccatcc agcgacaccg caaagccggc
    10021 agtgcgggca aaattgcctc cgctggttac gggcacaaca acaggcagtc gggtcacgcg
    10081 attaaaggcc gccggtgtga caatcagcac cggccgcgtt ccctgctgct catgacctgc
    10141 ggtaggatca agcgagacaa gccagatttc ccctctttcc atgtcagatt tcctcctgac
    10201 cagtcgccgg tgcatccagc cattctcgtt cttcagctga tatttcagca ttcggatcac
    10261 actgtgccag tagctcagcc agtgaatatt gcgggcgtct gtgcggctca acaatcagcc
    10321 ggccattatc aatgaccatg ccaacttcat tatctgtacc cagagacagc gcattcagca
    10381 gtgccggtgg aacggtcagc ataactgagc cgccaacctt cttcagtcgg gtggtatgca
    10441 ttcttcacct ccataaaagt tatatttaaa tataacatcc actaaaaaaa cacaccaggc
    10501 tttaacgcgc aatgtttaat aaaaatataa ctttcaacca aacagtaaac ccagcgtggt
    10561 tgcttccata tgcagcaata ccggcagcag caggccacca cttctgatcc tggccgctga
    10621 tgtaatcacc cccaccagga acagctctgc cagtgtcagc aggttcttat actggctatg
    10681 cgcggcgacg aacaacaacg acgttatcag cgcccccagc cacatcgtcc agcagtaccg
    10741 tgaacggaag acgttcagca taatcccccg gaacagcgtt tcctcattca acggggcaag
    10801 gataaagatg gtcagcaacg tcaggctcac gtcaggtata gacttatcgg caaaaagttt
    10861 cgtcataaat ggctcagcag gcagagccag caccttaccg agcagaaata caccgatata
    10921 caccacggcc atcgcaccga ccagccacgg tacgccaacg ttgcgcagct gaccaacgac
    10981 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac
    11041 agcaccatga ctgaagaaca aatagttttt tcctggccca taaagcagaa cggcctgctc
    11101 catgacaaat ctggctcccc agctaatgcc ccatgcagcc agcataacca gcataaactg
    11161 cagatattga ttacgtgtct gaatcattgt atctcctgta aatttttaac ttgtcctatt
    11221 tttgtcatta ctacatatat acacatgtat aacaattcag atatcgtcac caggatatgc
    11281 cgcgccagcg gcatggaagg cggcactccg ctgtttcata tgataccgcc gaagcccgat
    11341 gtaagccgct acagtcgtcc gaaagtcacc agcctcctcc ccctgccgtc atccgtgcat
    11401 cagctatgca ctgagtatgc ctgcccttcc ctagagaatc ctgccaggct tgccacactg
    11461 atatatcttg actttatgta aacgatatga cacctta
//
LOCUS       DAZFFI010000138        11453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFFI010000138 DAZFFI010000000
VERSION     DAZFFI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.138"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..380)
                     /gene="yfdP"
                     /locus_tag="ACHJ12_004275"
     CDS             complement(18..380)
                     /gene="yfdP"
                     /locus_tag="ACHJ12_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO4991975.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1126..1800)
                     /locus_tag="ACHJ12_004276"
     CDS             complement(1126..1800)
                     /locus_tag="ACHJ12_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4991976.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            1891..2091
                     /locus_tag="ACHJ12_004277"
     CDS             1891..2091
                     /locus_tag="ACHJ12_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO4991977.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2135..2686
                     /gene="ymfL"
                     /locus_tag="ACHJ12_004278"
     CDS             2135..2686
                     /gene="ymfL"
                     /locus_tag="ACHJ12_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO4991978.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2832..3011
                     /locus_tag="ACHJ12_004279"
     CDS             2832..3011
                     /locus_tag="ACHJ12_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4991979.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            2941..3519
                     /locus_tag="ACHJ12_004280"
     CDS             2941..3519
                     /locus_tag="ACHJ12_004280"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4991980.1"
                     /translation="MNALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGVGIG
                     VLELHTATIGALASFLLLQLSYTQIMVGWVGAPKGAPVSVCAGYANPAQFTTSEIGVS
                     GGGSYPLHTEAAIMATVPALSRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVET
                     LLNLQAERIRLADKANFHIHRLLNISGGHRHA"
     gene            3512..3748
                     /locus_tag="ACHJ12_004281"
     CDS             3512..3748
                     /locus_tag="ACHJ12_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4991981.1"
                     /translation="MLNPLILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            3745..4563
                     /locus_tag="ACHJ12_004282"
     CDS             3745..4563
                     /locus_tag="ACHJ12_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4991982.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCDSGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SSAANSPGSAGVAPGGGAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVKPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            4560..5054
                     /locus_tag="ACHJ12_004283"
     CDS             4560..5054
                     /locus_tag="ACHJ12_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4991983.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTHRHFPRLTERAQDPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EVLESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRNSSKRAASGEEWYLSGN
                     YVGA"
     gene            5054..5707
                     /locus_tag="ACHJ12_004284"
     CDS             5054..5707
                     /locus_tag="ACHJ12_004284"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO4991984.1"
                     /translation="MSNKYCQALAELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRYELEACGEAFLAQVRQQAEKLVREMAA"
     gene            5704..6030
                     /locus_tag="ACHJ12_004285"
     CDS             5704..6030
                     /locus_tag="ACHJ12_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO4991985.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            6027..6416
                     /locus_tag="ACHJ12_004286"
     CDS             6027..6416
                     /locus_tag="ACHJ12_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4991986.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRSRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            6436..7245
                     /locus_tag="ACHJ12_004287"
     CDS             6436..7245
                     /locus_tag="ACHJ12_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO4991987.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVEIVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7253..8242
                     /locus_tag="ACHJ12_004288"
     CDS             7253..8242
                     /locus_tag="ACHJ12_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4991988.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            8256..9008
                     /locus_tag="ACHJ12_004289"
     CDS             8256..9008
                     /locus_tag="ACHJ12_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4991989.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAESKARCERCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            9157..9233
                     /locus_tag="ACHJ12_004290"
     tRNA            9157..9233
                     /locus_tag="ACHJ12_004290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9191..9193,aa:Arg,seq:gcg)
     gene            9247..9323
                     /locus_tag="ACHJ12_004291"
     tRNA            9247..9323
                     /locus_tag="ACHJ12_004291"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9281..9283,aa:Arg,seq:tct)
     gene            9415..10374
                     /gene="stxA2b"
                     /locus_tag="ACHJ12_004292"
     CDS             9415..10374
                     /gene="stxA2b"
                     /locus_tag="ACHJ12_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO4991990.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            10387..10650
                     /gene="stxB2b"
                     /locus_tag="ACHJ12_004293"
     CDS             10387..10650
                     /gene="stxB2b"
                     /locus_tag="ACHJ12_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO4991991.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            11173..>11453
                     /locus_tag="ACHJ12_004294"
     CDS             11173..>11453
                     /locus_tag="ACHJ12_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4991992.1"
                     /translation="MAFKHYDVVRAASPSDLADTLAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMS"
BASE COUNT         2829 a         2650 c         3081 g         2893 t
ORIGIN      
        1 gttaattaag aacgatatta atgacctgct gcgcggagtt ttccgtcagg ctcaccggca
       61 agagtcagta attgtccctg gtcttcctgc agaatagtca ggcgaccacc gcgattgaca
      121 tacatcggcg tttcggtggt gtcttcttcg gaaattttcc cgcggttagt cgggcgaaca
      181 tatgagagtt tgtgtttgat tttcacacgg ttctcatcaa acggttcgat ttccaggttg
      241 agcgagacct tacctttggt tttcgtgttc atcacaccgg aagcgacttc actgagaact
      301 gcgccgattt ttgtttcaaa tacgccgccg tccagctccc cgataaatgc ctgcacatca
      361 gtactgcgtt cgctagccat tttgttgctc ctcatcatat cgaccctgca aggtcggttg
      421 gtttctccac aaaacagaga agaacacctg cggtggcagc cgcccggatg gattgggtta
      481 tgagcccgtc gtccggtgat gctcttctct gttttgtaaa aagagcggta ccagccggaa
      541 gcaagtgtac aaactggtac tgccaaagca gtggctgttg tggtgaccgg tgctgatctc
      601 cggcttgcgg ttatttcaga ctctcacggg cgtttgattg ccccgccgaa cagctctttt
      661 ccgcaatagc tgcaatgtct ttcgcgcatc agcctgcgca ttcaccacaa cgctgagagc
      721 attgccggtg tccgaatcga acggaccttt tccctgccca accctcccat ctgaatggga
      781 ctgtctggaa ttgaaccagc acttatgcct tgctcgtcaa tgctctcatc gttgcgtcct
      841 ggtctcttcc caggcgtcaa accgaatcgc cacgctggtt aggcgtctta tcagcatcct
      901 cattgacttg cacattccgg ctacctggtt tgtttgcccg agcaaggagt ggattgtccc
      961 ctttaacgtc cccagaccgc taacgacgca tgtgccatac gccgtgttac aaccaaattt
     1021 tgtttgaatc ttgcctgcct catgtttctt ttggatacat tatgtatctc atgggtacat
     1081 tgtcaagtat aaaaaaacct gccgaagcag gttcataaat attgattagg cctttattgt
     1141 gtatcttctt ggttttcccg agaaaatcac tgtaccaatt atagagcaat taccgttaat
     1201 cttaatgtaa ggctcaggcc agtttgggtt taatgctttg agataacgct gtgttccatc
     1261 ttctatcaat cgcttgaagg tggtttcgcc tgtatcgtgc atcaatgcaa taacgtcgtc
     1321 accgtggcag gcagggactt cgggatcaac aaaaatcatg tctccagggc ggtactcatc
     1381 aatcattgaa tcaccaatca cccgcaagat ataagtcatt tcgccacagg gtacagggca
     1441 gggatacgtt tctgctgtgc tcaaatcaac ctcagaatag ccaacttctt tccatgctcc
     1501 ggcctgtacc catgatatga cagggactaa cgttatttgt ttgttagtga ttgaaacatc
     1561 aggttttttt gtgatgttcg ttgtctggtg ttcttgatca agccatccga caggcaggtc
     1621 gaaacatttt tcgatgtgcc gtgccatgct gtcaccgata tttttagtag cgccatcccc
     1681 cataaacctg ctggtctggg ttggctcgcg atcaatcatg gtggcaaagg atgaatttcc
     1741 gccaacacca tctctcagtt ttctggcgtt agaccgccgg atgtcatgga ttgttttcat
     1801 aacgaaatta aaacctttgt accgataagg tacaagtatc ttgaaggttc atttcaatca
     1861 tgtaatatgt atatcggagg tacatattgt atgaaagcgt attgggactc tttaaccaaa
     1921 gaacagcagg gcgagttggc cggaaaagtt ggctcaacac ctggctactt acggctggtt
     1981 ttcaatggtt ataaaaaagc cagttttgtg ctggcgaaaa aacttgagca atgcacgtca
     2041 ggtgcaatta cgaaatctga cttaagaccg gatatctatc cgaaagatta acagaacacc
     2101 ttcaattttt aaccacagaa cgatgaggct aaccgtgggt aagtatcact ggaaagtaga
     2161 aaaacagcct gagtggtacg tgaaagctgt cagaaaaact atcgcagcgt tgccgggtgg
     2221 ttacgctgaa gcagctgact ggctggatgt aacagagaac gcattattta accgccttcg
     2281 tgccgatggc gatcagattt tcccgctggg atgggcaatg gttttacagc gcgcggctgg
     2341 cactcactac attgcggatg ctgtcgcaca gtctgctggt ggggtgttcg tatcgcttcc
     2401 tgaaattgag gaagtagaga acgccgatat aaaccagcgc ctgctggaag tcatcgaaca
     2461 gatcgggaat tactcaaagc agattcgttc ggcaatcgaa gatggggtcg tggagccaca
     2521 cgagcagaca gcaattaatg atgagttgta tctgtcaatt tcgaagctcc aggagcatgc
     2581 agcactggtc tacaaaatct tttgcgctcc agaaaagagt gacgcccgcg agtgtgcagc
     2641 tccgggcgtc gtggcgtttt gtgtctgtgg agaaactaac gcatgaacag tttaacggca
     2701 aataaccgtt tgtcgcaaca gctggtggtc agcgtcgctg aacacctgtt gttacggcat
     2761 gtatgcagat taccaaatca cctggctgta agtaaccaca gagaacttta cctgactgtg
     2821 gggggcgagt tgtgcaggaa cttaaccgct ggtttcgtga cggaagaggg ctttatgtcc
     2881 atgttattcg ttgggagcca gaaacacagc gcgttatcta tcttcgcaaa gactacccgc
     2941 atgaatgctt tagtcctttg tggaaattca ggcgtgattt tgttgagtgt gaaggaccac
     3001 cagcacattg attctgccat tccgggacgt tacactgttc aggcacctta taaagcgggt
     3061 gtcgggattg gcgtcctgga attgcatacg gcgacaattg gcgcgttagc gtcttttttg
     3121 ttgctacaac tcagctatac ccaaattatg gtgggctggg tgggggcacc gaaaggtgcg
     3181 ccggtttccg tatgcgccgg ttacgccaac cctgctcagt tcaccaccag cgaaattggc
     3241 gtttccggtg gtggaagtta tccattgcat acggaggctg ccatcatggc tactgtccca
     3301 gccctctctc gtctgaatga tgaagactta cataaactca gttatgtaac aactgcacta
     3361 cgtgctctgc gcaaggtaac tctttcggat ccgcaggcgc atcaggtttt ggtagaaacc
     3421 cttcttaact tgcaggctga acgtattcgt ctggcggata aggctaattt tcatattcac
     3481 cgtctcctga atatcagcgg agggcatcgt catgcttaat ccgttgatcc tcaatatttg
     3541 ccgtttgctt cagcgtaaaa aaacatcaat tcctacagtt gggcagtggt acaccacgcc
     3601 tgcagggcat gttctacgtg tcagcctggt tgaccgtgaa tgtcagaagg tgatttgtga
     3661 accgctgggc cgtaattacc gcgtcagtat gccgcttata gcctttcgct ccggaaaaaa
     3721 catgaagcat ctcggaggtg cagcatgagt atggagctga tggttaaagc gatgaaaatt
     3781 cgagtgggta atccattgcg aaaactggtt ctgatcaagc tggctgataa tgccagcgat
     3841 cagggtgagt gctggcccag ctaccagcat attgctgacc agtgcgagat tagcaaacgt
     3901 tctgtgatga atcatattgc ggccctttgt gattccgggc tggtaaaaaa agtcacccgg
     3961 aaaggtgaaa aaggtaactc aagtaatatc tatctccttc atcttgatgg tgcaggagat
     4021 tcactagggg gtagtgcaaa taattcacta tctagtgcag caaattcacc aggtagtgca
     4081 ggagttgcac cagggggtgg tgcaggagat tcacccagaa ccagtcactc ttttgaacca
     4141 gtcaaagaac cagtcaatga accaatagct gttggtgcat ctgctgatga gtccgtgcga
     4201 gttcgttcaa accgaccgga atactctccg gagtttgagc aggcatggct ggcatacccc
     4261 aaacgtgctg gtggcaattc aaaatctgca gccttcaaag cctggaaagc tcgtttgaat
     4321 gagggggtaa aacccgaaac catgctggaa ggtgtgaaac gctacgcggg ctgggtatct
     4381 gcgatgggta acagcggcac acaatttgtg aaacaggctg tcacgttctt tggtccggat
     4441 cgtcatttcg aagaatcctg ggaagttcct gcggtatctg cagccagacg cgaggacccg
     4501 tacttcaaag ccagttacga caacgtggac tacagccaga tcccggcagg attcaggggg
     4561 tgatcatgag tcttttgaat gaagttcaga aattcattga agcccatccg gggtgtactt
     4621 ccggagacat tgcggatgct tttgcaggtt actcacggca gcgcgttctg cagtcagcaa
     4681 gcaagttacg tcagagtggg cgtgtggctc accgttgtga aggagataca cacagacatt
     4741 tcccgcgcct gactgagaga gcgcaggatc cggaaccaca accagttcgt gaaaccagac
     4801 ctgtgcgcaa tttctatgtc ggcactaacg acccgcgggt gattttgtgc ctgacccgcc
     4861 aggcggaagt actggagtcc aggggcttat accgtcgtgc tgcaacggtg tggatggcgg
     4921 cattccgtga aagccactcc cagccagaac gaaacaattt tctggcgcgt cgtgaacggt
     4981 gtttacggaa tagcagtaag cgggctgcat caggtgaaga gtggtatctc tcagggaatt
     5041 acgtgggggc ttaatgagta ataaatattg ccaggcgctg gcggaactgc ggaacaaacc
     5101 agcccatgaa ctgaaggaag tgggcgatca gtggcgcacg ccggacaaca ttttctgggg
     5161 aattaacact ctgtttggcc cgtttgttct ggatctgttt actgacggtg ataacgccaa
     5221 atgtgctgcg tattacacgg cggaagacaa cgcgctggcg catgactggt cagaacgcct
     5281 tgcggagctt aaaggtgctg cctttggtaa tcccccatac agccgcgcca gtcagcatga
     5341 ggggcaatac atcaccggca tgcgttacat catgaagcat gccagtgcca tgcgtgataa
     5401 aggcgggcgc tatgttttcc tgatcaaagc tgccaccagc gaagtgtggt ggccggaaga
     5461 tgcagatcat attgctttta ttcgcgggcg tattggtttt gaactgcctg cctggtttat
     5521 cccgaaggat gagaagcagg tgccgacagg cgctttcttc gctggtgcta ttgctgtttt
     5581 cgacaagacc tggaagggac cggcaatcag ctacatcggg cgctatgaac ttgaggcatg
     5641 tggtgaggcc tttctggcgc aggttcgcca gcaggcagaa aaactggtca gggagatggc
     5701 ggcatgacga cgttaactca atgccagcag caggtgctgg atatgctgat ttcttatcag
     5761 aaagaacgtg gcttcccgcc aaccaatcag gaggtggcaa ccatgctggg ataccgttca
     5821 gtgaatgcag cggtggagca tcttcgcgca ctggagaaaa aaggcgtcat cacgataaag
     5881 cgtggcgtgg cccggggcat cacgcttcat accgcggtga aggacgacga cagcgaggcg
     5941 gtcgggatta tccgctcact gcttgccggt gaggaaaacg caaggctgcg tgcaacccac
     6001 tggttacatg agaggggcct gaaagtatga agctgatcct gccttttccg cccagcgtga
     6061 acacgtactg gcgacacccc aacaaagggg cgtttgctgg taagagcctg ataagcgcgg
     6121 cggggcgaaa attccagagc gcggcgtgcg cagcaatagt tgagcagtta cgtcgtctgc
     6181 cgaaaccaac gtcggcacct gcttcagtgg agatcgtgtt gtttcctccg gataaccgga
     6241 gccgcgatct ggacaactat aacaaggcgc tgtttgacgc cctgacccac gcgggggtgt
     6301 gggaagacga cagtcaggtg aaaagaatgc tggtggagtg gggaccggtt atcccggaag
     6361 ggaaggtcga gatcactatc agtaagtacg agaaaacggc gggtgcagct gcctgatcaa
     6421 gaggagaaac gaagtatgaa taatctgatg gtcattgatg gtattgaagt tcgtcgtgat
     6481 gcttatgggc gttacagcct gaacgatctg catcgcgcag cagtagcatc tggtgcaaat
     6541 gccagaacca aggagccagg aaagtttcta tccagccaac aaactgttga gattgttcat
     6601 gaattaacca acacccagaa tttgggtgtt gacccggtga gtgtgattca tgggggaaat
     6661 gaacggggaa cgtatgtctg taaggaactg gtgtatgcct atgcaatgtg gatcagtccg
     6721 tcattccatc tgaaggtgat ccgtactttc gacatggtaa ccagcgcgcc ggaaaagtta
     6781 tccggacagg ctgctgacaa gatgcaggct ggcgtgattc tgctggactt tatgcgccgg
     6841 gagttaaatc tgtctaactc atctgtgctt ggggcctgtc agaaactcca ggaggctgtt
     6901 ggcttaccga atctggcacc gcgctatgcc attgatgcac ctgctgacgc gcctgatggc
     6961 tcaagccgcc cgacgctgtc gctgagtgca ctgctgaagc agtatggtat ccgcctgacg
     7021 gctaatcagg catatcacca gatggcgaag ctggggatcg ttgaacaacg cgaacgatac
     7081 agccgtaccg cgattaacaa catcaaaaaa ttctggtcgc tgacagcgaa aggttgcatg
     7141 ttcggcaaga acatcaccag tcccgcaaat ccgcgcgaga cgcagccgca tttcttcgaa
     7201 tcccgattcc ctgagctgtt aaagctgctc gataccgttc attgaggtga ccgtgagagc
     7261 actactgacc cctgaaattg ccccgcgtat ggggatcgta ttgttcaggc caggttcaga
     7321 gctgatgccc ctgtttatgc aggggcgtgt cctgctggag cctgagccgg agcgttattc
     7381 atctttcgcc agtggtgccg ttccggcggc atcacaaccg ctggcggatg atcctgccgt
     7441 tcgggccgtg ttccgcaatg aggcagtgat ccgtcgtgct ggtggcgtgg aatgtcttga
     7501 aagctggtta cttcgtgaaa aaggctgcca gtggcctcat tccgactggc acagcgagaa
     7561 catgaccaca atgcgacacg ctccgggcgc aatccgtctg tgctggcact gcgataacca
     7621 gctgcgcgat cagttcacgg aacggctgga atcaatggcg acggataact gtgcccgctg
     7681 ggtgttgtct gttgtgcgtc gggatctcgg ttttgatgat agtcacgttg tgacaatgcc
     7741 ggaactgtgc tggtggctga ttcgtaatga cttggcggat gccttaccgg aaagtgcagc
     7801 ccgtaaggca ctgagattac caaatcttgt tgtgccgtct gtcacccggg aaagtgacct
     7861 tgtgccttcg gttcctgcca ccagcatcat ccaggataag gcaaaaaagg tgctggcgct
     7921 gaaagtggat ccggagtcgc cggagtcttt tatgttacgc ccaaaacgtc gccgctgggt
     7981 taatgaaaag tacacgcgct gggttaagac acagccgtgt gcatgttgtg gaaagcccgc
     8041 tgatgatccc caccacctga taggtcacgg tcagggtgga atgggaacaa aagcgcatga
     8101 cctctttgtg ttgcctttgt gtagaaagca tcacgacgag ctgcatgcgg ataccgtggc
     8161 attcgaagag aagtatggct cccagctgga gctgatattt cgttttatcg atcgtgcgct
     8221 ggcaataggc gtactggcgt aagtggagaa cgagcatgaa ccttgaagcc ttaccaaaat
     8281 attactcccc aaaatctcca aaattgagcg atgacgcacc ggcgacaggc tcgggtggtt
     8341 taacgattac agatgtgatg gctgcgcagg ggatggtgca gtcgaaagca ccgcttgggt
     8401 ttgccttatt cctggcaaaa gttggtgttc aggatcctca gtttgcgatt gaaggtctgc
     8461 tcaattacgc gatggcactg gataacccga cattgaataa attgagtgaa gaaacccggt
     8521 tacagatcat cccttacctt gtgaattttg cctttgctga ttattcccgg tctgcggaaa
     8581 gtaaggctcg ctgtgagcgt tgtgctggta ctggatttca taatgtattg cgcgaagtgg
     8641 tgaaacactc cagaagcggg gaatctgtta tcaaggaaga gtgggtgaag gaactatgtc
     8701 agcattgcca tggtaaggga gaagtcagca cagcgtgcag agggtgtaag ggtaaaggta
     8761 ttgtcctgga tgaaaaaaga acccggcttc atggcgcgcc tgtttataag atttgtgggc
     8821 gttgcaatgg aaaccggttt agccgtttac caaccacact ggcgaggcat cgtgtccaga
     8881 agctgatacc agacctgacg gattatcagt ggtacaaagg atatgcagat gtcattgata
     8941 aactggttac aaagtgctgg caggaagaag cgtatgctga agagcaattg agaaaagtga
     9001 caagatagat aatttcgccg aagatgtcga catgatgctt gcatttttca aaaaatatgg
     9061 ataaagtttt cccaacgatg ggctttgtat gtctaccgtt gataagattt aagagcccgc
     9121 cgctgagcgg gtttttgttg catctcctgt taaataccgc cattagctca tcaggataga
     9181 gcgccatcct gcgaagctgg ttgcgcgggg ttcgagtcct cgatggcggt ccattatcgg
     9241 tacactgcgt tgttagctca gccggacaga gcaattgcct tctaagcaat cggtcactgg
     9301 ttcgaatcca gtacaacgcg ccataattat ttaccaggct cgcttttgcg ggcctttttt
     9361 atatctgcgc cgggtctggt atcgtattac ttcagccaaa aggaatacct gtatatgaag
     9421 tgtatattgt taaaatgggt actgtgcctg ttactgggct tttcttcggt atcctattcc
     9481 cgggaattta cgatagactt ttcgactcaa caaagttatg tctcttcgtt aaatagtata
     9541 cggacagaaa tatcgacccc acttgaacat atatctcagg ggaccacatc ggtatctgtt
     9601 attaaccaca ccccaccggg cagttatttt gctgtggata tacgagggct tgatgtctat
     9661 caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa ataatttata tgtggccgga
     9721 ttcgttaata cggcaacaaa tactttctac agattttcag attttgcaca tatatcagtg
     9781 cccggtgtga caactgtttc catgacaacg gacagcagtt ataccactct gcaacgtgtc
     9841 gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact cactggtttc atcatatctg
     9901 gcgttaatgg agtttagtgg aaatgccatg accagagatg catccagagc agttctgcgt
     9961 tttgtcactg tcacagcaga agccttacgg ttcaggcaaa tacagagaga atttcgtctg
    10021 gcactgtctg aaactgctcc tgtttatacg atgacaccgg aagaagtgga cctcacactg
    10081 aactggggga gaatcagcaa tgtgcttccg gagtttcggg gagagggtgg tgtcagagtg
    10141 gggcgaatat cctttaataa tatatcagcg atactgggca cagtggcggt tatactgaat
    10201 tgccatcatc agggggcacg ttccgttcgc tccgtgaatg aagagataca accagaatgt
    10261 cagataactg gcgacaggcc agttataagg ctaaacaata ctttatggga aagtaatacc
    10321 gcagctgctt ttctgaatcg cagggctcac tctttaaata catccggaga ataacgggag
    10381 ttaaatatga agaagatatt tgtagcggct ttatttgctt ttgtttctgt taatgcaatg
    10441 gcagctgatt gtgcaaaagg taaaattgag ttctctaagt ataatgagaa tgatacattc
    10501 acagtaaaag tggccgggaa agagtactgg actaaccgct ggaatctgca accgctactg
    10561 caaagcgcac agttaacagg aatgacggta acaatcaaat caaatacctg tgcgtcaggt
    10621 tcaggatttg ctgaagtgca gtttaattaa tatcagaagc attgctggtt tcgtagtgtg
    10681 cagcaatgta gttacagtgt aatcaatgtc acaattcagt cagttgacgg ttgcctgtct
    10741 ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc cctgtgcgga ggggcataat
    10801 gacagatgtt tggttgcgta ttgtataggc aagttgcgga ttctgtctgg tcattgcaga
    10861 attcaccggg aggcacccgg catcatgctg tatacagaga ttaggcatat atccaggctc
    10921 ctcatcgcag gagccttttt acatgcaaaa aaagcccgcg ccgggagacg cgggcagcaa
    10981 ggaataaaca acaaaacgtg aagtaatcaa tttttcagct ggcgaataat acccgacagt
    11041 aatcactctg cgcaactgcg cggccttttt cgtattgcgg gctgtagtct tccttctgtc
    11101 attgtcctgt aacttccgga cctcagcccg ccccttatct gactcacaac attatcccgg
    11161 ccgggaggat tcatggcatt taaacactat gacgtggtca gggcggcgtc gccgtcagac
    11221 cttgctgata cacttgctca aaaaattcgt gaaggatggc agccatacgg cgggccgttt
    11281 tcttcgtata cggatgatgg cgcagcactt attcaggcga ttgtcgcaga aggtgatgtg
    11341 agcacacctg tggtggtgaa gccgtcggat ggagaaggca cagtaatcag cgccaccagc
    11401 gacccggagt attactttgt tgtggttctg gcagggcagt caaacggcat gtc
//
LOCUS       DAZFFI010000139        11420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFFI010000139 DAZFFI010000000
VERSION     DAZFFI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.139"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..431
                     /locus_tag="ACHJ12_004295"
     CDS             <1..431
                     /locus_tag="ACHJ12_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4991993.1"
                     /translation="GHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFA
                     SLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSK
                     FRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            446..1303
                     /gene="menB"
                     /locus_tag="ACHJ12_004296"
     CDS             446..1303
                     /gene="menB"
                     /locus_tag="ACHJ12_004296"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4991994.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1303..2265
                     /gene="menC"
                     /locus_tag="ACHJ12_004297"
     CDS             1303..2265
                     /gene="menC"
                     /locus_tag="ACHJ12_004297"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4991995.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFVRETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2262..3617
                     /gene="menE"
                     /locus_tag="ACHJ12_004298"
     CDS             2262..3617
                     /gene="menE"
                     /locus_tag="ACHJ12_004298"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4991996.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQRVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHNYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPIELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEVHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3727..3993
                     /gene="pmrD"
                     /locus_tag="ACHJ12_004299"
     CDS             3727..3993
                     /gene="pmrD"
                     /locus_tag="ACHJ12_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4991997.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKAAGKTQ"
     gene            complement(3987..4373)
                     /gene="arnF"
                     /locus_tag="ACHJ12_004300"
     CDS             complement(3987..4373)
                     /gene="arnF"
                     /locus_tag="ACHJ12_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4991998.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIATLLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4373..4708)
                     /gene="arnE"
                     /locus_tag="ACHJ12_004301"
     CDS             complement(4373..4708)
                     /gene="arnE"
                     /locus_tag="ACHJ12_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4991999.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4705..6357)
                     /gene="arnT"
                     /locus_tag="ACHJ12_004302"
     CDS             complement(4705..6357)
                     /gene="arnT"
                     /locus_tag="ACHJ12_004302"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4992000.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMSLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVIGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6357..7247)
                     /gene="arnD"
                     /locus_tag="ACHJ12_004303"
     CDS             complement(6357..7247)
                     /gene="arnD"
                     /locus_tag="ACHJ12_004303"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4992001.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIALGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7244..9226)
                     /gene="arnA"
                     /locus_tag="ACHJ12_004304"
     CDS             complement(7244..9226)
                     /gene="arnA"
                     /locus_tag="ACHJ12_004304"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4992002.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARISS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9226..10194)
                     /gene="arnC"
                     /locus_tag="ACHJ12_004305"
     CDS             complement(9226..10194)
                     /gene="arnC"
                     /locus_tag="ACHJ12_004305"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4992003.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10198..11337)
                     /gene="arnB"
                     /locus_tag="ACHJ12_004306"
     CDS             complement(10198..11337)
                     /gene="arnB"
                     /locus_tag="ACHJ12_004306"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4992004.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2784 a         2957 c         3065 g         2614 t
ORIGIN      
        1 gcgggcatcc ggggctgcaa aatgctgaac aacgtgcgga acgtcagcgt tccgatcgcc
       61 aatgggcgca gcgtttttgc acagaaccgt taacggcggt atttgccgac tggtatcaac
      121 agcctgtttt tgcctcgctc aatgacgatc aacgccggga gctggtggcg ctgcgcagca
      181 acaataatgg cgcaaccctt gccgccatgc tggaggcgac ttctctcgcc gtccagcctg
      241 atttacgtgc taaccttagt gcccgcacat ttgcgtttta ttatttatgt ggtgaacgtg
      301 acagcaaatt ccgcgccctg gcggcggaac tggctgccga ctgccatgtc attcctcgcg
      361 ccggacataa cgcgcatcgg gaaaatcccg ctggcgtaat cgcaagtctg gcgcagatct
      421 tgcgtttctg actaaaggac acaatatgat ttatcctgat gaagcaatgc tttacgcacc
      481 ggttgaatgg cacgactgct ccgaaggttt cgaggacatt cgttatgaaa aatccaccga
      541 cggtatcgca aaaatcacca ttaatcgtcc gcaggtgcgc aatgccttcc gtcctctgac
      601 ggtaaaagag atgatccagg cactggcaga tgcgcgttat gacgacaata tcggcgtgat
      661 cattctgact ggcgcaggcg ataaagcgtt ctgctccggt ggtgaccaga aagtgcgtgg
      721 tgattacggc ggctataaag atgattccgg cgtacatcac ttgaacgtgc tggacttcca
      781 gcgtcagatc cgtacctgtc cgaaaccggt tgtcgcgatg gtggctggct actccatcgg
      841 cggtggtcac gttctgcaca tgatgtgcga cctgactatc gcagcagata atgccatctt
      901 cggtcagact ggcccgaaag tcggttcctt cgacggcggc tggggcgctt cctacatggc
      961 tcgtatcgtc gggcagaaaa aagcgcgaga aatctggttc ctgtgccgtc agtacgacgc
     1021 aaaacaggcg ctggatatgg gccttgtcaa caccgtggta ccgctggcgg atctggaaaa
     1081 agaaaccgtc cgttggtgcc gcgaaatgct gcaaaacagc ccgatggcgc tgcgctgcct
     1141 gaaagctgcg ctgaacgccg actgtgacgg gcaggcgggg ctgcaggagc tggcgggcaa
     1201 cgccaccatg ctgttctaca tgacggaaga aggtcaggaa ggtcgcaacg ctttcaacca
     1261 gaaacgtcag cctgatttca gcaaatttaa acggaatccg taatgcgtag cgcgcaggta
     1321 taccgctggc agatccccat ggacgcgggg gtggttctgc gcgacaggcg gttaaaaacc
     1381 cgcgacgggc tgtacgtttg cctgcgtgag ggcgagcgcg aagggtgggg ggagatctcc
     1441 ccactgccgg gcttcagcca ggaaacctgg gaagaggcgc aaagcgtgct gcttgcctgg
     1501 gtaaataact ggctggcagg cgattgcgag ctaccgcaga tgccttccgt tgcctttggc
     1561 gtaagctgtg cattggcgga gctggcagat acgctcccgc aggcggccaa ttaccgtgcg
     1621 gcaccactgt gtaatggcga tcctgacgat ctgatcctca aacttgctga tatgccaggc
     1681 gagaaagtgg cgaaggtcaa agtgggattg tacgaagccg tgcgcgacgg catggtggtt
     1741 aatctgttgc tggaggcaat tccggatctg catttgcgtc ttgacgcaaa tcgcgcctgg
     1801 acaccgctga aaggccagca gtttgccaaa tatgttaacc cggattatcg ccaccgcatc
     1861 gcgtttctcg aagagccgtg caaaacccgc gatgattcgc gagcgtttgt ccgtgaaacc
     1921 ggcattgcca ttgcctggga tgaaagcttg cgcgagccgg attttgcctt tgtggctgaa
     1981 gagggcgtgc gcgcggtagt tatcaaaccc acgctcacgg gcagtctgga gaaagtacgc
     2041 gagcaggtac aggcggcgca cgcgctgggg ctgacggcgg tgatcagttc ttccattgaa
     2101 tcgagcttag gcttaacgca actggcgcgg attgccgcct ggttaacgcc ggacaccatt
     2161 ccagggctgg acacgctgga tctaatgcag gcgcagcagg tacgtcgctg gccgggtagc
     2221 acgctgcctg tcgtggaagt tgatgcactg gagcggttgt tatgatcttc tctgactggc
     2281 cgtggcgtca ctggcggcaa gtgcggggag aagccatcgc cttacgtctt aatgacgagc
     2341 aactcaactg gcgcgagctt tgtgctcgcg tcgatgaatt agcctccggc tttgcggcgc
     2401 agggcgtagt tgagggcagc ggcgtgatgt tgcgggcgtg gaatacgccg caaacgctgc
     2461 tcgcctggct ggcgttactg caatgcgggg cgcgggtgtt gcccgtgaac cctcagctgc
     2521 cgcaaccgtt gcttgaagaa ttgctgccca atcttacgct gcaatttgcg ctggtgccgg
     2581 aaggggaaaa cacgtttccg gcgttagcgt cgctgcacat tcagcgggtt gaaggcgcac
     2641 atgccgctgc gtggcagccg acgcgtctgt gctcaatgac gttaacctct gggtccactg
     2701 gcttgccgaa agccgctgtt cataattacc aggctcatct tgccagcgcc gaaggggtgt
     2761 tatcgctgat tccgtttggc gatcacgatg attggttgct ctctttaccg ctgtttcacg
     2821 tctccggtca gggaattatg tggcgctggt tatacgctgg tgcgcggatg acggtacgtg
     2881 ataaacagcc attggagcaa atgctggcag gctgtactca cgcttcactg gtgccaacac
     2941 aactctggcg tttgctggtt aaccgcagtt ccgtttccct gaaagcggtg ttacttggcg
     3001 gcgcggctat cccgatcgag ttgacggaac aggcgcgcga gcaggggatt cgttgctttt
     3061 gcggctatgg tctgaccgag tttgcctcca cggtgtgtgc gaaagaagcc gacggactgg
     3121 cagacgttgg ttcgccgctg ccgggtcggg aagtgaaaat cgttaataat gaagtgtggc
     3181 tgcgggctgc cagtatggca gaaggttact ggcgtaacgg gcaactggtt tcactggtta
     3241 atgacgaagg ctggtacgct acgcgcgatc gcggtgaggt gcataatggc aagctgacca
     3301 ttgtcggacg tttagacaat ctattcttca gtggcggaga gggtattcag ccggaagaag
     3361 tcgagcgcgt aattgctgca catcctgcgg ttttgcaggt gtttatcgtc cccgttgccg
     3421 acaaggagtt tggtcatcga ccggtggcgg tgatggagta tgaccacgag agcgttgatc
     3481 ttagtgaatg ggtgaaagat aagctggccc gttttcagca accggtgcgt tggttaacgt
     3541 tgccgcctga actgaaaaac ggcggtatta aaatttcacg tcaggcgcta aaagagtggg
     3601 tgcaacgtca gcaataatta accctccgtt tcacttctca tttctgccat tccattgccg
     3661 ttgataaaga gtttatctgt taatctgtaa attgatgtga aaactcttag caaacaggat
     3721 aatgcaatgg aatggctggt caaaaaatcg tgctgcaata aacaagataa cagacatgta
     3781 cttatgctct gcgatgctgg cggcgcgata aaaatgatcg ccgaagtgaa gagtgacttc
     3841 gccgtgaaag tgggggattt actctcgcct ttgcagaatg cgctttattg tattaatcgt
     3901 gaaaagctgc atacggtgaa agtcctctcg gcaagcagtt atagccccga tgagtgggaa
     3961 cggcagtgta aagcggcagg gaaaactcag taacgttgtt ttgtcgtggg caggaaaatc
     4021 agcatcaacc cgctcataat acaggctact cccagtagtg ctttcagcga aaaagtccct
     4081 tcccagccgg gaagtaccat tgaagcaatc cataccagca cataactcat actgagcaag
     4141 gcataggctt tgcttagcgc aagtttatgt agtgttttat accaacaaaa aacggaaagc
     4201 agataaccca gcagcccgag taacagtatc cgggcatcca ggccaaaagc taacagcgtc
     4261 gcgataaaat cccataagtg cgtcatcggc ggcagatgac tcgccgcaaa acccaggctt
     4321 aattgcgcaa ctgaggcaat aatgacgctg aataatcccc acatcaggcc cattacaccg
     4381 tactcccgag gatcacaatg ccaccaataa tgaacgccac cccacaccag tgacgcggcg
     4441 ataccggttc gtgccacagt tttactgcag ccagcgtcac ccagacaaaa ttcagactta
     4501 acatcgggta ggcaatgcct accggtacgt tctgcaagac cagcagccag agcaccatgg
     4561 caagaccaag acaagccagc gccagtccca gccacagcac gatatgtttg cgccgtttgt
     4621 ttatcgccac aaagcaggtt gcctgtttct gacacaactg cccggcaacg ctaagcaagc
     4681 tggcaaagac taatgttagc cagatcattt gggacgatac tgaatcagca ccagacgctc
     4741 ctgacgatcg atggcatctg cgggcggaat ggcgagactg ttgatatctt catcgcggtc
     4801 aaccgaaagc acgagtgtaa taatcccctc ctgacgatgt tgattaagcc agtttgcgaa
     4861 ctcatcaccg ctgacaaatc tccctttcgc atccggataa ttaaggccgt atttcaactc
     4921 acctgtctgg cgatacatga tgatgtcatc gcgttgcagg ctccatgcca gacctgcggc
     4981 aacaccgacg ctatcagtaa gaatatagcg gcttggctga agtgattctt gtgtcatctc
     5041 gacaaaaaat tgcggatgtt ttccttccat gactctgtca ggaattgaaa atcctaccag
     5101 caacgccagc cccagcgggc aaagcgcggc aaaagaccag gtcttttcga cgtttgttaa
     5161 ggtgtaccag ccgaaaaatg cccatagcga aaaaatcgac caggcacaaa agactttata
     5221 gctctcgaag gtttgccaca ccggcgtgtt cattggtccc cagggggaga ccacaaatgt
     5281 ggcaataatg ccaatgacgc caaaagcgat gttaatccag ccattaatcc gcaacgccag
     5341 aggattattt tttgctgcca gcaaagcgta atgcgccatc agcattgcca gaggtgcaaa
     5401 gcaggaaaga atataggtgg gcaatttgcc tttagcgacg gagaaaaaca gcagcgacat
     5461 tatcgtccag ctcaacaaat agacggttgc ggaatgcttg cggtttttcc agcctgcgta
     5521 cagtgcaccg gggagtaatc ccagccacgg caggctaccg gcaatgatga ccggcacgta
     5581 gtaccagaac ggagctctat gttgggcatc atccagtgca aagcgttgaa tatgctcaac
     5641 ccagaaaaaa tagtgccaga agtcaggctc ccgctgcgct atcgccagtc cccaggggag
     5701 aaccgtcagt acgcaactga taaccgccag ccagccgtaa ataaagagat ctttccagcg
     5761 tttttgcgtc gctacccatg gcagtacgct taataccggc acggcaaggg cgagaaaacc
     5821 tttggtcatc acccccatgc cgcaggtgat tcccagcagt aaaaatcctg cgcttttgcc
     5881 tttccacgtc tgtgcctgca ttgccagcca gaagctgcac attcccgcca ccagccagaa
     5941 tgcaataaac ggatcgagca cggcataagt gccgatggca tagacaataa acaatgagag
     6001 ataaattact gtggcgagta gagccagacg tttatcgcgc cataagcgca gagtaaacca
     6061 ggtcaccagc gcggcagtta acagggtcgc aaagataacg cctgcccgca caccaaagtt
     6121 attcgcgcca aatagccatt gcccaatgct gttaatccag tatccggcaa tgggtttttc
     6181 gaaataacgt agccctaaca gatggggcac aatccagtcg ccggatgcca gcatttctcg
     6241 actgatttcc gcataacgcg tttcatctgg ttgccagagc agacgcgtgc tgatcggtaa
     6301 caggtaatag caggcaataa acgcgaagag gccgataagg taacgtaccg atttcatcag
     6361 cgactacccg caatttgttg gcaacccagc cagccttcac gtccggcaat atttccgcga
     6421 acaacttgtc cgagcggcaa cgtctctgat aacagttcgc ttaaagggca aaatgtcacg
     6481 ccttcctgag ctgcgcgttt gaggagatcc acaaaattat gctgataagc gcagccttca
     6541 acttctgcat gaatggtata aaccggcgtg cctttatctc gcaggatgcg gttgagtaac
     6601 caaccattaa aatcttctgc tttcacatcc cgaccaatca cttcgtccca ggtgggtaag
     6661 gtcaccggaa tttgcgcagt gccagggttt cctgattcga gcagtggacg gaacggcatg
     6721 gccccacggc aatcgctgtt gtagcgcaaa tggaacgctt cttttgcttc gatcacctgc
     6781 tggtcggcac gccagcccgc agcggcagaa caggttaccg gttgaccgat aatctcttcc
     6841 agggtgcgaa ggccgagtgc aatatcgtcg atcattgttt gccgatccca gttaccgcta
     6901 tgggcttgcc aggcatggtg atcccaggcg tgcaggccga cttcgtgatg tttagccgct
     6961 tcacgaatga tatcggcatt ggcatgacca atctctttac ctggccaggc cgtacctgcc
     7021 agtaaaatat cccagccata aagcgatgcc gcgtttgagc gcagcatctt ccacaaaaac
     7081 tgtggcttca ccagtcgcca gagatggcgg cccatattgt ccgggccgac gctgaaaaaa
     7141 atgctggcct gaatattatg cttactcaag atttccagca gacgcggcac gccttcacgg
     7201 gtgccacgaa aggtatcgac atcaatgcgt aagccgactt tggtcatgat ggtttatccg
     7261 taagatcaac ggtgcgcagg aagaaatcca gcgtttcgtc gatggtttcc tgcatatcaa
     7321 tttttggttc ccagtccagg cagcggcggg cattgcggat gctcggttta cgatgctcta
     7381 catcctgata tcctttgccg tagtagctgc tactttcgac aacgcgaaag cctgcaaacg
     7441 gcgggaagtg atggcgcagc ggatgttttt cgaagctcgc cagcagcatc tcgcccagtt
     7501 cctcaatgct cgcttcgttc tcaggattgc caatgttgat aatttcgccg tcgcagcgat
     7561 ttcccgcatt ttcgataatg cggtataacg cctcgatacc atcgcgaata tcagtaaagc
     7621 agcgtttttg ttttccgcca tcaatcagct taatcggtga accttctacc agattgagaa
     7681 tgagttgcgt aatagcgcgg gagctgctga ttcgtgccgc attaaggtta tccagtcgcg
     7741 gtcccatcca gttaaacggg cggaagaggg tgaactgtaa accctctttt tcgccatagg
     7801 cccagatcac ccgatcaagt aattgttttg ataccgaata aatccagcgt ggtttattca
     7861 ccgggccgac gattaaatta gaatggtcct catcgaagta tttatcgcta cacatcccat
     7921 aaacttctga agttgacggg aagatgattc gcttacggta cttcacgcag tagcggataa
     7981 tgcgcagatt ctcttcaaaa tcgagttcaa atacgcgcag cgggttgcgg gtatattcaa
     8041 tcggcgtggc tatcgccacc agcggcaaga cgacatcaca ttttttgacg tgatactcaa
     8101 tccattcgga atgaatactg atatcgcctt cgacaaagtg aaaatgcgga tgattcagaa
     8161 aacggcttat cgcatcgctg ccaatatcca gaccgtaaac ttcataatga tcttcgcgca
     8221 gcaggcgttc tgtcagatgg ttgccaataa agccatttac cccgaggatg agtacccggg
     8281 tacggcgtcg ggcggtgcag gcaggctggc tattcaagcg tgaaccttgc accaggccca
     8341 gcgtctgcgc taattgcgag ccctgcatag taatgccgtc gcccgcctgt ccggtgacga
     8401 tttccagcgc gccatcgcca caggcaatca acagcggtgc aatagaaatc acgctccccg
     8461 gctgtgcttt gctggcatga ggatgaacac gcgacgacca gacggtaaat ttctgattgc
     8521 caacatagct gaaggcaccc ggccacggat cggcaacggc acgtaccatg ttgtgcagta
     8581 cggatgccgg tttatgccat tcaaggaaac tgtcatccgg cgttctgcga ccaaaacagg
     8641 tggcttcgtt ttcgcgctgg gcgatttcca gaatattacc gtgtttaatg gcgggtaatg
     8701 tctgttccag tagctggcgc gcggcatggc acaatttatg atgcagcgta atagcgatat
     8761 cgtctggcgc aatggcaacg cgcagttgcg ccacaatggc cccagcatcg gcacgtttta
     8821 ccattcggtg caatgtaacg cccgtttccg tttcaccgtt caccagcacc cagttcagcg
     8881 gcgcgcgacc acgatatttt ggtaacagcg aaccatgcag attaaatgca cctgcgggag
     8941 cgagctgcaa aatttcgtcg caaataagat ggcgataata aaaagagaaa atcacatctg
     9001 gcgacagttg ggcaatgcgt tccacccaca gcggatgatt aacgtcatcc ggcgcataaa
     9061 ccggaatgcc tctttccgct gccagacgag ccaccgaacc ataaaaggct ttttcaccgg
     9121 gattatcggt atgggtaaaa atggcgctaa tttcgtaacc ggcagccagc agagcttcaa
     9181 taccgaggca tcccatatcg tggtaggcaa aaacgacggt tttcattatt cattttcctt
     9241 gctggatgga cggataactt gctgaacaaa atagcggggg cgggcgcgga catcggtgta
     9301 gatcctgccg atatattcac cgagtaatcc catgccgata aactgagcgc caataaaagt
     9361 aaacagcacg gcaaatagca taaagacgcc ttctgccgcc cattgtggtc cgaaggtcag
     9421 gcgtaaaatc accagcagca ccgcaatgct aaaacctcca atcgcaataa tgctgccgag
     9481 cagactcagc atacgtagcg gcgttgtggt aaggcaggtc accaggtcgt acatcaaatt
     9541 aatcaggcgc ataaaactgt atttggattc accaaactca cgctcggaat gatgtactgg
     9601 aatttcaatg gcacggcggg cgaagatatt cgccagaatc gggataaagg tgctacgttc
     9661 atggcagtgc aacatcgcat cgacaatatg acggcgatag gcgcgcagca tacaaccgta
     9721 gtcacccatc gctttgccgg tggtgcgctg aataagccgg ttaatcatct tcgaagctgt
     9781 tttacgaaac cagctgtcct ggcggttctg gcgtacagtc cctaccacgt cgtaaccttc
     9841 atcggctttt gccaccaggc gggggatttc ttctggcgga ttctggagat cggcatcaag
     9901 ggtaataatt aagtcgccgg taacgtgact gaatcccgcc ataatcgctg aatgttgccc
     9961 gtaattgcgg ttaagcaaaa tagacacaat atggctgttc tccgcttgtg aggcttcgac
    10021 cagcatatgc gcggaattat cgctactgcc gtcatcaatc agcaggatct catactcttt
    10081 ccccaacgat tcacaggctg tggtggtgcg cctgattaat tccggtaagc tttcctgctc
    10141 gttataaacg ggaataacca ccgagacttt cttaacaggg tggatttcaa acatggctta
    10201 ttgtcctgcg agttgctgaa gggctgtgat gacacggtcg gcatcggcgg tagtcatatc
    10261 cgggaacagc ggcaaagaac agatgcgttc gctattccat tcggtattcg gtaacgacag
    10321 cgtgggaaaa cgctcgcgat aatatttttg tgtgtgagcg gcgcggaaat gtaacccggt
    10381 accaatgcct ctttctttta acgcttccat caacgcatcg cgactgatac cacaacgttg
    10441 ttcatcgaca cgaataataa acagatgcca ggcgtgaacg tgcggccagg cgggaaggct
    10501 taatggctga aaggggagag ctgccagtgc ttgctgatat tgctgggcaa tttcgcgccg
    10561 acgggtgttg aggtgctcta attttgctaa ctgtgtcagg gcaattgcgg cgttaatatc
    10621 ggtcagattg tacttatagc ccggtgttaa gacttcagcc tgcggtgcac ggccccaggt
    10681 ttgtctgtca taggcatcga cacccagacc gtgaaatttc agcatccgta gctggcgggc
    10741 aaggttttca ttatcagtta caatcaggcc accttccgca caggtaatat ttttaatggc
    10801 atgaaatgaa aaaatagcgg taccttttgc gccaatatgt cgccctttgt aatacgtacc
    10861 gacggcatgg gcagcatctt cgataactgc gatgccgtaa cgttcgccaa tggcgcgaat
    10921 ggcgtcaata tctgctggcg caccggcata atgcaccgga atgatggctt tagtgcgtgg
    10981 cgttatggct gactcgatcg cttcaggcgt gaccatcagc gtatcgcggt cgacatccac
    11041 cattaccggc gttgcaccca acaaggaaat catattgagg gttgaaaccc aggtcaggga
    11101 aggcgtaatc acttcatcgc ccttgccaat ttccaacgcc attagcgtga tatgcattcc
    11161 ggcggtggct gaactgaccg cgatggcatg ctgatttccc gtcaactggc aaaaggcttg
    11221 ctcaagcgcc tgattcttcg gaccggttgt gagccaaccg gattcgagaa cctctttaac
    11281 tgcagcgagt tcctccacgc ccattgctgg tcgcgaaaaa ggcaaaaatt ctgacattgc
    11341 ttttccttcc gccattgaat acctgtccac ttatatttgc tataaagagt gttgtgtata
    11401 ttttgccatt tggagcgaaa
//
LOCUS       DAZFFI010000140        11260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFFI010000140 DAZFFI010000000
VERSION     DAZFFI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.140"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            412..1026
                     /gene="evgA"
                     /locus_tag="ACHJ12_004307"
     CDS             412..1026
                     /gene="evgA"
                     /locus_tag="ACHJ12_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4992005.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1031..4624
                     /gene="evgS"
                     /locus_tag="ACHJ12_004308"
     CDS             1031..4624
                     /gene="evgS"
                     /locus_tag="ACHJ12_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4992006.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4680..5825)
                     /gene="yfdE"
                     /locus_tag="ACHJ12_004309"
     CDS             complement(4680..5825)
                     /gene="yfdE"
                     /locus_tag="ACHJ12_004309"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO4992007.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5899..6843)
                     /gene="yfdV"
                     /locus_tag="ACHJ12_004310"
     CDS             complement(5899..6843)
                     /gene="yfdV"
                     /locus_tag="ACHJ12_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4992008.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6913..8607)
                     /gene="oxc"
                     /locus_tag="ACHJ12_004311"
     CDS             complement(6913..8607)
                     /gene="oxc"
                     /locus_tag="ACHJ12_004311"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4992009.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8661..9911)
                     /gene="frc"
                     /locus_tag="ACHJ12_004312"
     CDS             complement(8661..9911)
                     /gene="frc"
                     /locus_tag="ACHJ12_004312"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4992010.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10424..11056)
                     /locus_tag="ACHJ12_004313"
     CDS             complement(10424..11056)
                     /locus_tag="ACHJ12_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4992011.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3307 a         2515 c         2317 g         3121 t
ORIGIN      
        1 tatctctcat ttctcataga tgaaatttat gaattgaatg aaaagagcat tcattcttca
       61 tatcaaacac agtgcatgtg ttgcacatta tcccaggaac gttggaggcc taatttaaat
      121 gaggtgcaaa aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcct
      181 gtaggattag tgagaagact tatagtgcta acttgaaact ataaatcatc ggtataatcc
      241 ctgattttat tgttgacatt tcatttatgc cgactattta tatggtatac ttgtcgaatt
      301 atcttaaagg aagctcagat tttcttattt ttattgagaa aatgagatga cgccttatgt
      361 ctgtattact acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca
      421 ataattattg atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac
      481 gatattgaaa tcttagcgga gttgactgaa gggggaagcg ccgttcagcg ggtggaaaca
      541 cttaagcctg atatcgtcat cattgatgtc gatatccccg gagttaacgg tatccaggtg
      601 ttagaaacgc tgaggaagcg ccaatatagc ggaattatta ttattgtctc cgctaaaaat
      661 gaccattttt acgggaaaca ttgtgctgat gctggcgcta atggattcgt gagtaaaaaa
      721 gaaggcatga acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc
      781 cccttctctc tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc
      841 ttatcgaaac aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac
      901 attgctgaaa aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg
      961 gaaaaattag aatgtaaatc actgatggat ctttacacat tcgcgcaacg taacaaaatc
     1021 ggctaatcac atgaagtttt taccctattt ttttcttctc tgttgtggtc tttggtcgac
     1081 cataagtttc gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt
     1141 cacacttgat ccagtacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa
     1201 tcttgtgatt gcagtacaca agtcccaaac ggctaccttg ttgcataccg attcgcagca
     1261 acaggttcgt ggtattaatg ctgattattt aaatctttta aaaagagcgt taaatatcaa
     1321 attaacactc cgggaatacg ccgatcatca aaaagcaatg gacgcgcttg cagaaggtga
     1381 agtcgatata gtgttatcac atttagttac ttcgccgcct cttaatagtg acattgctgc
     1441 aaccaaacca ttgataatta cctttccggc gctggtaacc acccttcacg actcaatgcg
     1501 accgcttacc tcaccaaaac cagtaaatat tgctcgggta gcaaattacc ccccagacga
     1561 ggtaattcat caatcgtttc caaaagcaac aattatctct tttacaaatt tatatcaggc
     1621 attagcatcc gtatcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag
     1681 cagtatgatt tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcgcc
     1741 gcgtcaatat aattttttct tgaccagaaa agaatccgtc attcttaatg aagtactcaa
     1801 tagatttgtt gatgctttaa caaatgaagt tcgctatgaa gtttcacaaa attggcttga
     1861 tacaggaaac ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacagtg
     1921 gattaagcaa catcccgatt taaaggtgct ggaaaatcct tactctcctc cctattctat
     1981 gacggatgaa aatggctcgg ttcggggcgt tatgggggat attcttaata ttattacctt
     2041 gcaaacaggt ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca
     2101 gcttagcccc ggaggatggg atataatacc tggcgctatt tatagtgaag atcgagaaaa
     2161 taatgtttta tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc
     2221 gcctgacagt gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga
     2281 gctgcattcg caattaaaag agatgtatcc ggaggttgaa tggatacagg tcgataatgc
     2341 cagcgctgca tttcacaagg ttaaggaagg tgaacttgat gctctggtcg cgacacagtt
     2401 aaattcgcgt tacatgatcg atcattacta tcctaatgaa ctttatcatt ttcttattcc
     2461 tggcgttccg aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga
     2521 tattattaat aaagcactga atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa
     2581 atggattaaa atgcccaatg tgaccattga cacatgggac ctatatagcg agcaatttta
     2641 tattgttacg acattatccg ttttattagt tggcagtagc cttttatggg gattctacct
     2701 gttacgctca gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt
     2761 ccgaaaagca ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa
     2821 tgtcattagt cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc
     2881 aatgttacca ttagaaaaca gtgactcacc ctttaaagat gttttttcta atgcgcatga
     2941 agtcacagca gaaacgaaag aaaatcgaac aatatacaca caggtatttg aaattgataa
     3001 tggcatcgag aaaagatgca ttaatcactg gcatacatta tgcaatcttc ctgcaagtga
     3061 caatgcagta tatatttgtg gttggcaaga tattactgaa acgcgtgatc taattaatgc
     3121 actcgaggta gaaaaaaata aagcgataaa ggctaccgta gcaaaaagtc agtttctggc
     3181 aacgatgagt cacgaaataa gaacaccaat aagctctatt atgggcttcc tggaacttct
     3241 gtcgggttct ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctacgccac
     3301 cggacaatca ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg
     3361 taactatcaa cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca
     3421 ctctttcggt gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacatttcc
     3481 tgatcattac ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact
     3541 gagcaatgct ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca
     3601 cattgatgac aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc
     3661 gcaggaagaa caacaacaac tctttaaacg ctacagccaa acaagtgcag gtcgccagca
     3721 aacaggatct ggcttaggct taatgatctg caaagaatta attaaaaata tgcagggcga
     3781 tttgtcatta gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga
     3841 aattagccag caagtggcga ctgtcgaggc aaaagcagaa caccccatca cactacctga
     3901 aaagttgagc atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca
     3961 gctaaatcta ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa
     4021 agtcagtatg caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg
     4081 ttttgagttg actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac
     4141 agccaacgca caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt
     4201 gttcaaaccg ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc
     4261 gcatattgca cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa
     4321 cgatctacaa ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct
     4381 acccgctgcg tttcaagcac tagaagctgg cgataacaga actttccatc agtgtattca
     4441 tcgcatccac ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca
     4501 gttagaaata acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc
     4561 tgtaaaagaa cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga
     4621 ctaaatagcg gctcccacaa tgttcaaatg tgggagctat ttaccagcac atcttacatt
     4681 tatgatgaga actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc
     4741 ataacatgcg ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattccc
     4801 ccggcttcaa tcaacatatt tctcgcctga gtttgtggca atttaatggc ctcagccaca
     4861 cttaataacg gcgcgacggg tacaccaact tcatgtattc tggctaacca aacttcagct
     4921 gcctgcgttt ttaacgtccg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt
     4981 aaaatattgc tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat
     5041 aacgcagaaa aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtattg
     5101 aaaacatcaa aaggtgccat gtagggatgg cgatttccca gacgttgtgg tgacttccca
     5161 gttgcgatat atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct
     5221 atatcgacat gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattccg
     5281 ctgaataaat agacaccgcc gcatagatcc gcaagagagg taccaacgcg cactggcgga
     5341 gcatcaggat atcctgtttc catcattatc ccgctcattg cctgaatgat ggtatcgtag
     5401 gcaggagcat cttttagcgg accggtatgc ccgaaacctg acgatgaagc atatatgagg
     5461 cgcgggttga tttcttgaag cgtttcccat gaaaacccca gtttttccat tgtacctggg
     5521 cgaaaattct cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg
     5581 tgatcattct ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag
     5641 agtgactgtc catccacata gggaccaaat gtgcgggtat catcaccatg acccggcggc
     5701 tcaactttaa ttacccttgc gcccatatta caaagaagtt gagttccgaa aggtccatta
     5761 aggacatgtg tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt
     5821 gtcatttgct tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa
     5881 aatcaataac atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt
     5941 gacgacaaag cccagtacgc tcacagccaa tgacgctgta ccagtacggg tgtagacatt
     6001 aaaccggctg gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat
     6061 catctgcaga tgttcgctgt tcaaatgaca tgccatacca acgagaagca gtgccagtgg
     6121 catcagaatc agcttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg
     6181 tgcagccaga gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag
     6241 attaaaggtt gggtcccatg ctgccggaat ttttacccca accaacacca ggatcgttgc
     6301 cagaacaggt gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact
     6361 attcttctta ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt
     6421 aattgcgtta acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc
     6481 ataaatagga tcgagaactg caaaccccag gaatccaatg gtaggtgaac ccgcaattaa
     6541 tgcacataca gctgcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccagga
     6601 gaaaaagaaa catccgacaa taaccacaag tgatacaagg gtcagacgag tgtccgcaaa
     6661 aatcatttcc ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt
     6721 taataccagt ttattaaatg cgcgggcttg atcttctgaa aaagtttctc gtctgccgct
     6781 aaaataaccc aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag
     6841 cataatagta tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga
     6901 cgatgtcagt atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact
     6961 ttcagttcct gctgcagggt cgatgaccac attaataatg gtcggtttgc gcgactgaat
     7021 accggtggtt aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg
     7081 aaacgcatcc attaatttgt cataccttgc atggtgcaac agatccgttg gtgatggtgc
     7141 accagcgcca ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac
     7201 gatcgtcacc ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa
     7261 agcactatca ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag caccgatggc
     7321 atagcccata ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt
     7381 atacatatca ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata
     7441 aatatcctgg ttctcgcgca atacgtcgcg cacagcactt aatgcattaa agtaatttaa
     7501 tggttgagta tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat
     7561 atttaaaata tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gttctgccag
     7621 cataccttgc atactggatg caatatcacc aacgactggc acagcaatgg ggcggttgct
     7681 gtcaatttcc tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt
     7741 tttaccgtgt gccaataacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt
     7801 tgccagggca aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt caaggatccc
     7861 tttcgccata gacattggca ggaatggaat ctgagtactt tcaataaatt cacgaagctg
     7921 ttcatcagct tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt
     7981 tgctaaaagc gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg
     8041 attttcaact ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac
     8101 atttgctggc aaatcaagat aaactccgcc agggcgaccc gatacagaga cccggatagc
     8161 gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata
     8221 cggttttgcc gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat
     8281 cgcgcggtcg ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcgttggc
     8341 caatgcggtc aaaccattga ggaatcctgg cgcagaaact gtcaggcaga tccccggttt
     8401 ttgggtaaga aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc
     8461 aatataacga atgccttccg cctgggcatg gcgtgccata tccgtcacag gaatacctac
     8521 aacaccataa atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc
     8581 atctgtcatt tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga
     8641 gccagaagcc cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg
     8701 tcgctataac ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct
     8761 ttaatatccg gcgtaaaggc agagaatttc attggacaac caacggtcag atattttcca
     8821 cgcaacggtt gttccacttc aacaacactg ccactttggc gcaaagaggg atcaagtgaa
     8881 atttctttca tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc
     8941 gcttcatgtt tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata
     9001 tgtggctgtc gtgcatgggc tgtactgtat gccggatcgg taatccattc tggtttgccg
     9061 atggctttac aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta
     9121 ggatcggttt cccagccttt acatttcagg atccagccag gctgaccgcc accacctgca
     9181 ttaccaccgc ggggaactgc atcaccaaat gtaccattcg gatactgcgg gtattcttcc
     9241 agataaccca atttatcgag acgctgctgg tcacgtaatt tcacgcggca aaggttcaat
     9301 acggcatcct gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc
     9361 aaagcagcaa gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc caacgctgca
     9421 gcgcttacca gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc
     9481 tgagcaacgt tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg
     9541 atcgaaccaa aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg
     9601 tgatcaatgg cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt
     9661 tccattacct ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg
     9721 ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt
     9781 acgtcaccaa cgccgggacg ttcaatttta ataacgtcag cgccaaacca ggccagcatt
     9841 tgagtacaag atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt
     9901 ggagttgaca tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta
     9961 aggcgtaata tcttctataa ataacccgca aaatatcgtc gtagctgcac tcgatatttt
    10021 taaaatcctg tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc ctttctgcat
    10081 ggtaagtagt gaggacgaaa agaagaatat gataaatatt gattcgtggt aatcagagag
    10141 agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta
    10201 agggtatgat tacgcttttt aaagcgtacc ataaaacata acataataaa tgatttgtga
    10261 tgacacggga ttattgggat ttactttgag gaagataaaa tgcgtgttta tagaaccgcg
    10321 agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct
    10381 ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg
    10441 cttcgctgtc gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt
    10501 attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg
    10561 gcatcagata ttggttttcc attacgccaa caccggccag gcgaagttct tccattgcgc
    10621 cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg
    10681 gagttgctac ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat
    10741 cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg
    10801 acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca
    10861 gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct
    10921 ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg
    10981 gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca
    11041 taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata
    11101 ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac
    11161 agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg
    11221 tcgcagagaa ctattcttat gtaccacaaa gagatgtcat
//
LOCUS       DAZFFI010000141        11091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFFI010000141 DAZFFI010000000
VERSION     DAZFFI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.141"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..1418
                     /gene="manD"
                     /locus_tag="ACHJ12_004314"
     CDS             204..1418
                     /gene="manD"
                     /locus_tag="ACHJ12_004314"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO4992012.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1485..2768
                     /locus_tag="ACHJ12_004315"
     CDS             1485..2768
                     /locus_tag="ACHJ12_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4992013.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3020..3937
                     /gene="oxyR"
                     /locus_tag="ACHJ12_004316"
     CDS             3020..3937
                     /gene="oxyR"
                     /locus_tag="ACHJ12_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4992014.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3920..5320)
                     /gene="sthA"
                     /locus_tag="ACHJ12_004317"
     CDS             complement(3920..5320)
                     /gene="sthA"
                     /locus_tag="ACHJ12_004317"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4992015.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5651..6301
                     /gene="fabR"
                     /locus_tag="ACHJ12_004318"
     CDS             5651..6301
                     /gene="fabR"
                     /locus_tag="ACHJ12_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4992016.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6301..6660
                     /gene="yijD"
                     /locus_tag="ACHJ12_004319"
     CDS             6301..6660
                     /gene="yijD"
                     /locus_tag="ACHJ12_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4992017.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6700..7800)
                     /gene="trmA"
                     /locus_tag="ACHJ12_004320"
     CDS             complement(6700..7800)
                     /gene="trmA"
                     /locus_tag="ACHJ12_004320"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4992018.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAESLRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7914..8104
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8169..10013
                     /gene="btuB"
                     /locus_tag="ACHJ12_004321"
     CDS             8169..10013
                     /gene="btuB"
                     /locus_tag="ACHJ12_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4992019.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9958..10815
                     /gene="murI"
                     /locus_tag="ACHJ12_004322"
     CDS             9958..10815
                     /gene="murI"
                     /locus_tag="ACHJ12_004322"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4992020.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2565 a         2614 c         2960 g         2952 t
ORIGIN      
        1 gagtttgtta tgtgattatt taacgtgttg ttttaacgat aatggttaat tgttcaggtg
       61 gtttttgagg gtattttttc tcctcggtgg aaatttttgt ttttaatgtg atcacggttg
      121 aataatctct ttttctctct tgtatggtag taggcgtaaa attaagtttg aaacgtctat
      181 ttacaacaca ggagattgca ataatgaaaa ttattgctgc agatgttttt gttacttgcc
      241 cagggcgtaa tttcgttacg cttaaaataa ccactgaaag cggactttgt ggattaggag
      301 atgccacgtt aaatggtcgc gaattgtcgg tcgcttctta tctgaaagat catctttgtc
      361 cacaactaat tggccgcgat gccagccgta tcgaagatat ctggcaattc ttttataaag
      421 gtgcttactg gcgacgtggc cccgtcacga tgtcagccat ttctgccatt gatatggcgt
      481 tgtgggatat caaagctaaa gcggcaaata tgccattgta tcaactgctt ggtggtgctt
      541 ctcgcgaagg cgtgatggtt tattgccaca ccaccgggcg gacgattgat gaagtccttg
      601 aagactatgc aaagcaccag cagatgggct ttaaggcgat tcgtgtgcag tgcggtgtac
      661 cgggtatgca aactacttat ggcctggcga agggcaaagg cctggcgtat gaaccggcaa
      721 ccaaaggcct gtggccagaa gagcagttgt ggtcttcaga aaagtatctc gattttacgc
      781 cgaagttatt tgaagcggtg cgtaataagt tcggttttaa cgaacatctg ttgcatgaca
      841 tgcaccatcg cctgaccccc atcgaagcgg cgcgctttgg gaagagcatt gaggattacc
      901 gcctgttctg gatggaagat ccgacgcctg cagagaacca ggaatgtttt cgtctgattc
      961 gccagcatac cgtgacgccg attgcggtcg gtgaagtgtt taacagtatc tgggactgca
     1021 agcagttaat cgaagagcag ctgattgact atattcgcac gacgatcacc catgcaggcg
     1081 gcattaccgg gatgcggcgg attgccgatt ttgcatcgct ttatcaggta cgtaccggtt
     1141 cgcatggccc ttcagattta tcgcctgttt gtcatgcggc ggcgctgcat tttgacctat
     1201 gggtaccaaa ttttggcgtt caggagtaca tgggctattc cgaacaaatg cttgaagttt
     1261 tcccgcacag ctggcgcttc gaggaaggtt atatgcaccc aggcgatgaa ccggggctgg
     1321 gtatttcttt cgatgagaaa ctggcggcga aatatccgta tgacccggcg tatctccccg
     1381 tcgcacgact ggaagatggc acattgtgga actggtaata acggttcgct taattatttt
     1441 ctgaatagtc atcaaatcga ttaattccgc tcaaggagct aaaaatggac gttgatgtgt
     1501 caaccagtgt tgcgggtaat aaaccgcaac ggattcgtcg tattcagaca gtgaccctgg
     1561 ttttattatt tatggcgggg atcgttaatt ttctcgaccg ttcgtcattg agcgtggcag
     1621 gtgaagcgat tcgtggcgag ttaggattat cggcgacaga gtttggtgtt ttgctttctg
     1681 cattttctct gtcttatggt ttttcacaac taccttccgg tattttgcta gaccgctttg
     1741 gcccacgaat tgttttaggc gcaggcttaa tattctggtc attaatgcag gcattaacag
     1801 gaatggttaa ttcttttagc cactttattt taatgcgtat cggtctgggt attggcgaag
     1861 cgccatttat gcctgcggga gtaaagtcga tcaccgactg gtatgcacaa aaagaacgcg
     1921 gcacggcgct ggggattttt aactcatcta ccgttatcgg tcaggctatc gcgcctcctg
     1981 ctcttgtatt gatgcagctg gcatggggct ggcggacgat gttcgttatc atcggcgtgg
     2041 cagggattct ggttgggatc tgttggtacg cgtggtatcg caaccgggcg cagtttgtcc
     2101 tgactgacga agaacggacg tatctctccg cgccggttaa accgcgtcca cagctgcaat
     2161 ttagcgagtg gctggcgctg tttaagcatc ggacaacctg ggggatgatt ttaggtttct
     2221 ctggcgtcaa ttataccggt tggctctaca tcgcgtggtt acccggttat ttgcaagccg
     2281 agcaaggttt cagtctggcg aaaaccggtt gggtggcggc gattcctttc ctcgcggcgg
     2341 cagtcgggat gtgggttaac ggtattgttg tcgatcgact ggcgaaaaaa ggctacgacc
     2401 tggcgaagac gcgtaaaacg gctattgtct gcggtttgat gatgtcggca ttaggcacgt
     2461 tgctggtcgt gcaatcttcc tcgccagccc aggcggtagc gtttatctca atggcgctat
     2521 tctgcgtgca tttcgctgga acgtctgcat gggggctggt gcaggtgatg gtgtcagaaa
     2581 caaaagtggc ttccatcgcc gggattcaaa actttggcag ttttgttttt gcttcctttg
     2641 ctccgatcgt aaccggttgg gtagtggata ccacacactc gtttaatctg gcgttggtta
     2701 tcgcggcctg tgtgacgttc accggggcgc tgtgttactt ctttattgtc aaagatcgca
     2761 ttgagtaatc gttactatca ggctctcttg ctgtgggcct gtagaataaa aaaaagcgga
     2821 tcctggagat ccgcaaaagt tcacgttggc tttagttatt cgagttgaga aactctcgaa
     2881 acgggcagtg acttcaaggg ttaaaagagg tgccgctccg tttctgtgag caattatcag
     2941 tcagaatgct tgatagggat aatcgttcat tgctattcta cctatcgcca tgaactatcg
     3001 tggcgatgga ggatggataa tgaatattcg tgatcttgag tacctggtgg cattggccga
     3061 gcaccgccat tttcggcgtg cggcagattc ctgccacgtt agccagccga cgcttagcgg
     3121 gcaaattcgt aagctggaag atgagttggg cgtgatgttg ctggagcgga ccagccgtaa
     3181 agtgttgttc acccaggcgg gaatgctgct ggtagatcag gcgcgtaccg tactgcgtga
     3241 ggtgaaagtc cttaaagaga tggcaagcca gcagggcgag acgatgtccg ggccgctgca
     3301 cattggtttg attcccacag ttggaccgta cctgctaccg catattatcc ctatgctgca
     3361 ccagaccttt ccaaagctgg aaatgtatct gcatgaagca cagacccacc agttactggc
     3421 gcaactggac agcggcaaac tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc
     3481 attcattgaa gtgccgttgt ttgatgagcc aatgttgctg gctatctatg aagatcaccc
     3541 gtgggcgaac cgcgaatgcg taccgatggc cgatctggca ggggaaaaac tgctgatgct
     3601 ggaagatggt cactgtttgc gcgatcaggc aatgggtttc tgttttgaag ccggggcgga
     3661 tgaagataca cacttccgcg cgaccagcct ggagacgctg cgcaacatgg tggcggcagg
     3721 tagcgggatc actttactgc cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt
     3781 tgtttatctg ccgtgcatta agccggaacc acgccgcact attggcctgg tttatcgtcc
     3841 tggctcaccg ctgcgcagcc gctatgagca gctggcagag gccatccgag caagaatgga
     3901 tggccatttc gataaagtgt taaaacaggc ggtttaaacc gtttaacgca gctacccgat
     3961 aggcttccgc catggtcggg tagttaaagg tggtgttgac gaagtactca atagtgttgc
     4021 cgccaccttt ctgttccata atcgcctgac cgatatgaat aatttcggca gcgcgctcgc
     4081 caaagcagtg aatacccaga atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc
     4141 ccacgttcat gccgacgatt tgtgcgcgtg ccagatgttt aaactgggcg cggcccactt
     4201 catatggcac tttcatcgcg gtcagctgct gttcggtttt gcccacagag ctgatttccg
     4261 ggatggtgta aatgccggta gggatatctt caatcagatg cgcggtggct tcgcctttta
     4321 ccagcgcctg cgcggcaatg cgcccctggt cataggccgc tgatgccagg ctcggataac
     4381 caatcacgtc gcccaccgcg taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct
     4441 tcagctgtcc gcgactgtca gtttccagcc caatgttctg taacgccagc gaatcggtat
     4501 taccggtacg accgttggca tagagcaggc agtcagcttt cagtttttta cccgacttca
     4561 gatgcatgat cacaccgtcg tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa
     4621 tcactacgcc actgttccag aagtgatagg agagagaatc tgacatctct tgatcgagaa
     4681 atgccagcag gcgatcgcgg gtgttgatca gatccacttt tacatccata ccgcggaaga
     4741 tcgacgcata ttcacagccg atcactccag caccatagat aagtacatgg cgcggttcgt
     4801 ggtgcatgct aagaattgag tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg
     4861 ttggatgata tggacgagag ccgcaggcaa taacaaattt ttcagcggtt agtgtttcaa
     4921 cgctgccgtc cgggcaatcc agcgccaacg tatgctcgtc aacaaagcga gcgtttccct
     4981 gcaatatttc acagtgatta cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa
     5041 tcacgttatc ggcatggtta aggatatcgg caaaagaaga gcggagcagt cgggaatggt
     5101 cgctgtaaag tgggttttga ttgaattcta taatgcggct gacggcgtga cggagagctt
     5161 tcgacgggat ggtgccccag tgggtgcaac cgccgccaac attttgataa cgctcgataa
     5221 ctgcgacgcg cgcaccttgc ttaaccaggc ccattgcagc gccttcgccg ccggggccgg
     5281 aacctattac tatggcatcg taatcgtagg aatgtggcat ggtagggctt acctgttctt
     5341 atacataaaa gcaacagaat ggtaacattt tatcgcgggt aagccaattg atccccgtca
     5401 tttatctggc tatatcctga gcggcctttg ctttgtctgt ttcttacttt tgccctgacg
     5461 ttttattgga tttttatcga cgatactctc cgtttaagcg gcaggtttcc gctgtacgta
     5521 aaagaaccgg ccaaagaatt gcagtaaata tgttttattg cgttaccgtt cattcacaat
     5581 actggagcaa tccagtatgt tcattctctg gtatagtgcc agcagtactt ttggcaagga
     5641 ttcagacatc gtgatgggcg taagagcgca acaaaaagaa aaaacccgcc gttcgctggt
     5701 ggaagccgca tttagccaat taagtgctga acgcagcttc gccagcctga gtttgcgtga
     5761 agtggcgcgt gaagcgggca ttgctcccac ctctttttat cggcatttcc gcgacgtaga
     5821 cgaactgggt ctgaccatgg ttgatgagag cggtttaatg ctacgccaac tcatgcgcca
     5881 ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgc acctcggtct ccacatttat
     5941 ggagttcatc ggtaataatc ctaacgcctt ccggttatta ttgcgggaac gctccggcac
     6001 ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag cacttcattg cggaacttgc
     6061 ggactatctg gaactcgaaa accatatgcc gcgtgcgttt actgaagcgc aagccgaagc
     6121 aatggtgaca attgtcttca gtgcgggtgc cgaggcgttg gacgtcggcg tcgaacaacg
     6181 tcggcaatta gaagagcgac tggtactgca actgcgaatg atttcgaaag gggcttatta
     6241 ctggtatcgc cgtgaacaag agaaaaccgc aattattccg ggaaatgtga aggacgagta
     6301 atgaaacaag caaatcaaga tagaggtacg ctgctgctgg cgttagttgc tggcttatcg
     6361 attaatggta ctttcgcagc gctgtttagc tccattgtgc cattttctgt attcccgatt
     6421 atttccctgg tgctgacggt ttactgcctg catcaacgtt atcttaatcg cactatgccg
     6481 gtaggcttgc cgggtctggc agctgcctgt tttattctcg gcgtactgct gtacagcacg
     6541 gtagttcgtg cggaatatcc ggatatcggc tctaacttct tcccggcagt actctccgtc
     6601 attatggtgt tctggattgg cgcgaagatg cgtaaccgta agcaggaagt tgctgagtaa
     6661 tcggtattat gccggatgct ggcgcatccg gcatgggttt tacttcgcgg tcagtaatac
     6721 gccgcactcc atatggtgcg tgtaggggaa ctgatcaaac agagccagac gttcgacctt
     6781 gtgtgtctgg cttaatgttt ccagattctt gcataacgtt tccggattgc aggagatata
     6841 cagaatacgt ggatacgcct gcaccatttt ctcggtttca ctgtccagac cgctgcgcgg
     6901 tgggtcgaca aaaatggttt cgcactgata actctttaag tcgatccctt gcaggcggtt
     6961 aaactcgcgc acaccattca tcgcctgagt aaattcttct gccgccatac gaataatttg
     7021 tacgttatca atatggttag ctgcgatgtt gtattgcgca gcagcaaccg acggcttagc
     7081 gatttcggtg gctaataccc gatcaaaatt gcgcgccagt gctaatgaaa agttaccgtt
     7141 gccgcagtac agctccagta aatcgccttt tgagcctttg gttacgtcca gcgcccattc
     7201 cagcatctga atattcatcg ccgcgttcgg ctgggtaaaa ctgttttcta cctgacggta
     7261 gatcatctct ttccctgcga ccggcagacg ttcatcgatg taatcttgat ccagctcgat
     7321 tttggttttc gttgcccgac caatcagatg cacattcaga ttctgtgctc gcagtgcatc
     7381 gcgcagggat tccgcttcct gacgccactc atcatccagc tttttatggt acagcaggga
     7441 aaccaccgcc tgattactca gcgtggtgag gtaatcaatc tggaacaact tgtggcgcag
     7501 aacgggatta ttacgcacac ccgcaatcat cgccgtcatc aactggttga tgagttcact
     7561 ggcggcgggg aagctatcca cgcggatacg gcttttggtt tgttgatcga aaatgatgtg
     7621 atacaggtca tcgccatcgt gccagatgcg gaactccgca cgcatccggt aatgactgac
     7681 cggcgagcga aacacttccg gaaccaggtc agaaaacggt gccatcatac tttgcaaacg
     7741 taccactttt tcggctaact gcgcttcata ctgttctgtt ggaaggtgtt cgggggtcat
     7801 gatgtatcct gaaaaattaa agtacgcggc gattgtaggg attgctcatc agatgtccag
     7861 atcttgatga attcctattt gtgagctacg tctggacagt aacttgttac aacctgtagc
     7921 atccacttgc cggtcctgtg agttaatagg gaatccagtg cgaatctgga gctgacgcgc
     7981 agcggtaagg aaaggtgcga tgattgcgtt atgcggacac tgccatccgg tgggaagtca
     8041 tcatctctta gtatcttaga tacccctcca agcccgaaga cctgccggcc aacgtcgcat
     8101 ctggttctca tcatcgcgta atattgatga aacctgcggc atccttcttc tattgtggat
     8161 gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg ttctgtcaca gccttttccg
     8221 cttgggcaca ggataccagc ccggatactc tcgtcgttac tgctaaccgt tttgaacagc
     8281 cgcgcagcac tgtgcttgca ccaaccaccg ttgtgacgcg tcaggatatc gaccgctggc
     8341 agtcgacctc ggtcaatgat gtgctgcgcc gtcttccggg cgtcgatatc acccaaaacg
     8401 gcggttcagg tcagctctca tctattttta ttcgcggtac aaatgccagt catgtgttgg
     8461 tgttaattga tggcgtacgc ctgaatctgg cgggggtgag tggttctgcc gaccttagcc
     8521 agttccctat tgcgcttgtc cagcgtgttg aatatatccg tgggccacgc tccgccgttt
     8581 atggttccga tgcaataggt ggggtggtga atatcatcac gacgcgcgat gaacccggaa
     8641 cggaaatttc agcagggtgg ggaagcaata gttatcaaaa ctatgatgtc tctacacagc
     8701 aacaactggg ggataagaca cgagtaacgt tgttgggcga ttatgcccat actcatggtt
     8761 atgatgttgt tgcctatggt aataccggaa cgcaagcgca gacagataac gatggttttt
     8821 taagtaaaac gctttatggc gcgctggagc ataactttac tgatgcctgg agcggctttg
     8881 tgcgcggcta tggctatgat aaccgtacca attatgacgc gtattattct cccggttcac
     8941 cgttgctcga tacccgtaaa ctctatagcc aaagttggga cgccgggctg cgctataacg
     9001 gcgaactgat taaatcacaa ctcattacca gctatagcca tagcaaagat tacaactacg
     9061 atccccattt tggtcgttat gattcgtcgg cgacgctcga tgagatgaag caatacaccg
     9121 tccagtgggc aaacaatgtc atcgttggtc acggtagtat tggtgcgggt gtcgactggc
     9181 agaaacagac tacgacgccg ggtacaggtt atgttgagaa tggatatgat caacgtaata
     9241 ccggcatcta tctgaccggg ctgcaacaag tcggcgattt tacctttgaa ggcgcagcac
     9301 gcagtgacga taactcacag tttggtcgtc atggaacctg gcaaaccagc gccggttggg
     9361 aattcatcga aggttatcgc ttcattgctt cctacgggac atcttataag gcaccaaatc
     9421 tggggcaact gtatggcttc tacggaaatc cgaatctgga cccggagaaa agcaaacagt
     9481 gggaaggcgc gtttgaaggc ttaaccgctg gggtgaactg gcgtatttcc ggatatcgta
     9541 acgatgtcag tgacttgatc gattatgatg atcacaccct gaaatattac aacgaaggga
     9601 aagcgcggat taagggcgtc gaggcgaccg ccaattttga taccggacca ctgacgcata
     9661 ctgtgagtta tgattatgtc gatgcgcgca atgcgattac cgacacgccg ttgttacgcc
     9721 gtgctaaaca gcaggtgaaa taccagctcg actggcagtt gtatgacttc gactggggta
     9781 ttacttatca gtatttaggc actcgctatg ataaggatta ctcatcttat ccttatcaaa
     9841 ccgttaaaat gggcggtgtg agcttgtggg atcttgcggt tgcgtatccg gtcacctctc
     9901 acctgacagt tcgtggtaaa atagccaacc tgttcgacaa agattatgag acagtctatg
     9961 gctaccaaac tgcaggacgg gaatacacct tgtctggcag ctacaccttc tgaaccacgt
    10021 cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt cggtctatga cgagatccgg
    10081 catctcttac cggatctcca ttacatttat gctttcgata acgtcgcttt tccgtatggc
    10141 gaaaaaagcg aagagtttat tgttgagcga gtggtggcaa ttgtcaccgc agtgcaagaa
    10201 cgttatcccc ttgcgctggc tgtggtcgct tgcaacaccg ccagtaccgt ttcacttcct
    10261 gcattacgcg aaaagttcga cttcccggtt gttggtgtcg tgccggcgat taaacctgct
    10321 gcacgtctga cggcaaatgg cattgtcgga ttactggcaa cccgcggaac tgttaaacgt
    10381 tcttatactc atgagctgat cgcgcgtttc gctaatgaat gccagataga aatgctgggc
    10441 tcggcagaga tggttgagtt ggctgaagcg aagctacatg gcgaagatgt ttctctggat
    10501 gcactaaaac gtatcctacg cccgtggtta agaatgaaag agccgccaga taccgttgta
    10561 ttgggttgca cccatttccc tctactacaa gaagaactct tacaggtgct gccagaggga
    10621 acccggctgg tggattctgg cgcagcgatt gctcgcagaa cggcctggct gttagaacat
    10681 gaagctccgg atgcaaaatc tgccgatgcg aatattgcct tttgtatggc aatgacgcca
    10741 gaagctgaac aattattgcc cgttttacag cgttacggct tcgaaacgct cgaaaaactg
    10801 gcagttttag gctgatttgg ttgaatgttg cgcggtcaga aaattatttt aaatttcctc
    10861 ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca acggcaaaca
    10921 cgccgccggg tcagcggggt tctcctgaga actccgacag agaaagcaaa aataaatgct
    10981 tgactctgta gcgggaaagc gtattatgca caccccgcgc cgctgagaaa aagtgaagcg
    11041 gcactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat a
//
LOCUS       DAZFFI010000142        10994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFFI010000142 DAZFFI010000000
VERSION     DAZFFI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.142"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..1887)
                     /gene="glmS"
                     /locus_tag="ACHJ12_004323"
     CDS             complement(58..1887)
                     /gene="glmS"
                     /locus_tag="ACHJ12_004323"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4992021.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="ACHJ12_004324"
     CDS             complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="ACHJ12_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4992022.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3770..4189)
                     /locus_tag="ACHJ12_004325"
     CDS             complement(3770..4189)
                     /locus_tag="ACHJ12_004325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4992023.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="ACHJ12_004326"
     CDS             complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="ACHJ12_004326"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4992024.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="ACHJ12_004327"
     CDS             complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="ACHJ12_004327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4992025.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="ACHJ12_004328"
     CDS             complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="ACHJ12_004328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4992026.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="ACHJ12_004329"
     CDS             complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="ACHJ12_004329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4992027.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="ACHJ12_004330"
     CDS             complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="ACHJ12_004330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4992028.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="ACHJ12_004331"
     CDS             complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="ACHJ12_004331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4992029.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="ACHJ12_004332"
     CDS             complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="ACHJ12_004332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4992030.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="ACHJ12_004333"
     CDS             complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="ACHJ12_004333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4992031.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2615 a         3007 c         2732 g         2640 t
ORIGIN      
        1 atgtcagtta taaaaaaggg ccaccggtcg gcagcccttt tttatctatg atagtgatta
       61 ctcaaccgta accgattttg ccaggttacg cggctggtca acgtcggtgc ctttgatcag
      121 cgcgacatgg taagccagca gctgcagcgg aacggtgtag aagatcggtg caatcacctc
      181 ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac ccgcatcctg
      241 atcggcgaag acatacaact gaccgccacg cgcgcgaact tcttcaatgt tggatttcag
      301 tttttccaac agttcgttgt tcggtgcaac gacgataacc ggcatatccg cgtcaatcag
      361 cgccagcggg ccgtgtttca gctcgcctgc agcgtaggct tcagcgtgaa tgtaagagat
      421 ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac ggcccaggaa
      481 cagcgcgtga tgtttgtcag agaaatcttc tgccagagct tcaatgcgtt tgtcctgaga
      541 cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt catgttcaat
      601 ggaggcatcc agacctttca ggcgagacag cttcgccacc agcatcaaca gcacagttaa
      661 ctgagtggtg aatgctttag tggatgccac gccgatttcc gtacccgcgt tggtcatcag
      721 cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg ccagcgaacc
      781 aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg tttcgccaga
      841 ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttgcgat agcggaattc
      901 agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt aacgggaaac
      961 cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa ccttcgacag
     1021 cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc tgatgcgccc
     1081 ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc tctttctgca tgtagtgacg
     1141 gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct gacgttttac
     1201 ttccgcgcca gttttatcga agatgtttac cgaacggcga gtaatttccg caatatcgcc
     1261 ctcttcaagg aagataaagc gacgggtcac cggcaacagc gccagctggt cagaagcgat
     1321 aaagttttcg cccataccca ggccaatcac cagcggacta ccagaacgtg ccgccagcag
     1381 ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccacgca gttgcgggat
     1441 agcacgcaga acagcctcac gcagagtacc gccctgtttc agctcccagt tcaccagatg
     1501 ggcaattact tcggtgtctg tttcagaaac gaaggtatag ccacgcgctt ttaactcttc
     1561 gcgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt gttcagaaac
     1621 atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag tatgagcaat
     1681 acccgtaccg ccatgcagag gatgttcttc cgctgcctga gccagcatct ggactttacc
     1741 gaggcgacgc aggcgggtca tatgaccttc cgcatcaaca acggccagac cggcagagtc
     1801 atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta catcacgttg
     1861 cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc gttgtcggtc
     1921 aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg ttatttttat
     1981 agttactgct tgtgggaggg agattatgtt atctcctcat cccagatgac cggattaccc
     2041 ggccagaatt actttttctt taccggacga cgccagcctt ctttctgagt ctgcggcaca
     2101 cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc cgcagcaatg
     2161 gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc gacaaacaca
     2221 tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt aatggttccc
     2281 gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag atgaccagct
     2341 ttcgagcctt tacccagacg cgcttttttc atctcaacaa agttaccgac gtgtgcacct
     2401 tccagcaact cagcaccagg acgcagacgg gcaaacgggc caatagtaca ggccgctgcc
     2461 agattcgcgt cttccacaac ggtatacgga ctgatttcgc aatcatcgcc aatcacgctg
     2521 tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac gttgccctcg
     2581 atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc gcgcagatca
     2641 aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc ctgttcggac
     2701 tggtaaacgc gctccagacg ggagagttgc aggcggttat tcacaccttc tacttcgctt
     2761 aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag cgcaataatg
     2821 tcggtgatgt agtattcacc ctgagcgtta ttgttggtca gcttcgccag ccagcgtttc
     2881 atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg acgctgctcg
     2941 tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cattttcacg ggtgatacgt
     3001 ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc ctgcggttta
     3061 gcatcacgca ggcgctggag tgtttcgaca gagatcagcg gcacgtcgcc gtagagcatt
     3121 aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcattgcatg acccgtaccc
     3181 agctgctctg cctgaagcac ccagttaagg ttgtcgtctt tcagcgcctg tttaagcaga
     3241 tcgccgccgt gaccgtacac caggtgaacg tgcgctgcgc ctaattcatt cgcagcatca
     3301 atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg aagatcggaa
     3361 tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc tcatagcatt attcaacata
     3421 cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa aaactacatt
     3481 tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc cccgctgacg
     3541 aaagtgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag gcagtacctt
     3601 catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa acgggaaaac
     3661 aggggtgttt tatatcagca ggatctatgt gaacgctctt caggacggat cacgcgtaaa
     3721 aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt tacatcgctt
     3781 ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc gcagacgcct
     3841 gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta cgtttcgctt
     3901 ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc agaacggtca
     3961 cgttgccagg ctgcacttca agaatgccgc cagacagata gataaactct tcgtgaccgt
     4021 gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt gcgtggccag
     4081 ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc agaccagaga
     4141 acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata tcaccctccg
     4201 attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac cgaccatgta
     4261 gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa agccacggat
     4321 ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg ccacgaagaa
     4381 cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt tgtcttcttc
     4441 agacagttca tccataccca ggatggcgat gatgtctttc agttcctgat aacgttgcag
     4501 gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca gcgggtccag
     4561 ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag acgcgatctg
     4621 acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag acgggtcagt
     4681 caagtcatcc gcaggtacgt ataccgcctg tacggaggtg atagaaccag ttttggtgga
     4741 ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat aacctaccgc
     4801 tgaaggcata cggcccagca gtgcggatac ttccgtaccg gccagggtgt aacgatagat
     4861 gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag ccatggtcag
     4921 accggtcaga gcaacgcgca gacggtttcc cggcggctcg ttcatctggc catacaccag
     4981 ggatactttg tcgataacgt tggagtcggt catttcgtgg tagaagtcgt taccctcacg
     5041 agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg cgatgttacg
     5101 aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca gaccaacttt
     5161 accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt ccagcagttc
     5221 ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc aacgctcttc
     5281 ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga tacggcccag
     5341 agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat ccagaccgcg
     5401 acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccac cgagctgctg
     5461 ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat cgtacacgcg
     5521 cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct ggacaatctt
     5581 tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc ggcccccgag
     5641 acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta taccaactgc
     5701 agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac catacgggcg
     5761 gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc gacataacga
     5821 cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc ccaggattta
     5881 tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat ggtcggaacc
     5941 tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag acggccttcg
     6001 tcgtaggcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga agggttatcc
     6061 cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa cgacacgcct
     6121 ttcgagccga tcattgcgag gtcgcattga acgcctttgt cggtccaggt cttcatttcc
     6181 gccagcagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg gtcggtcgac
     6241 accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg cttatattcc
     6301 agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata aggacggctg
     6361 gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc catcgcttta
     6421 gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt tgcgccggcc
     6481 atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga cgttaccagg
     6541 attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt tcgtcgttgt
     6601 agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg acgtaagcca
     6661 gcagagcggc ttcgaagctg ccaattttcg acagttcaac atccgccagg taaccacgtt
     6721 ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac tgtttctgtt
     6781 tcagcagttc ggtcactttc tgaccgtggt caagctgctt acgtgttgca tcgtcaaggt
     6841 cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg gtacggatac
     6901 caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg gatacggaaa
     6961 tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc aggaagatct
     7021 gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca cccgcctgag
     7081 tttcgataat cggcagtgcg gtcagagaac cggttttccc tttcacttca cctttggtga
     7141 aggcttcaac gtattcggcg ttaacacgtg cagcacgctc cagcagacga gagtggaggt
     7201 agaaaacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc agggagatct
     7261 gacggtaagc aacagcctgt ttagacaggt catcgtaaat gatcagcgca tcttcaccgc
     7321 ggtcacggaa gtattcgccc attgcgcaac cggcatacgg tgccaggtat tgcagtgcag
     7381 cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg tgctcttcca
     7441 gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca tagatacatt
     7501 tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt ttacctgtct
     7561 gacggtcacc gatgatcaat tcacgctgac cacgaccgat tgggatcatg gagtcaacgg
     7621 ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg cccggagcga
     7681 ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg attggtgcac
     7741 ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc aggatacggc
     7801 cagtacactt aactttcatg ccttcggcaa ggtcagcgta cggacccata acaaccgcac
     7861 ctacagagtc gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc agggagatca
     7921 tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca cttacagaaa
     7981 caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg cgctgcttga
     8041 tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa gactgcaaga
     8101 cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata tcacccgctc
     8161 ggatgataac gcctgccatt acagacttat cgattttgca attcagctta actttgcgtg
     8221 acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc agtgcggcag
     8281 cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga ataaactgct
     8341 ccagaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga atcaggttct
     8401 gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggcg agcgtttctg
     8461 gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc tcggcggcaa
     8521 acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag tcaaaagctg
     8581 ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc ctccttacag
     8641 ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg aacgttcgat
     8701 gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct cttcacgggc
     8761 acgtttacgc tcggcttcaa tttctgcctg cgcctgggcc acgattttag tacgttcctg
     8821 ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg cctgctcgat
     8881 gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg cctttgcaag
     8941 gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt ctttttgacg
     9001 tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca gaacgaacag
     9061 gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac aatgcctcta
     9121 tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac gcgacagcga
     9181 acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc agacccataa
     9241 cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct gcgccttcca
     9301 ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc agacccatca
     9361 tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca gtctccagtt
     9421 tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc catcgacaga
     9481 tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag gatgtggaaa
     9541 atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc agcaatcaga
     9601 atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccaag tgaaactggt
     9661 ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc ccagtgattg
     9721 aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt gatgctgtag
     9781 aacagaatca ggataaatac gcccaatgcc atagacagcg ttacgttcac gtccgcagac
     9841 ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg cagcaggtcg
     9901 ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt cagggccagc
     9961 ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt aacaaagccg
    10021 atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc ctttttggct
    10081 acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa catggagtca
    10141 atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga gaatgtacgc
    10201 aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt catattttct
    10261 gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt atctgaacca
    10321 ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa accgccaacg
    10381 ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcgccaaat gcgaatgtcc
    10441 aggccacccg gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat atcataaaca
    10501 aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc cagaaggggt
    10561 ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg agcagaagct
    10621 tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact cctgctccct
    10681 tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa gcatcaaaaa
    10741 gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca aaaggtgaat
    10801 aaatgtttaa atatttttcc agtgcataca attgcgactt ttctgctaac cctgttcgat
    10861 catgaaaaac tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca gatcacatat
    10921 tgcgcatgtt cgcgcacagc atatttattt acttggcaaa tgatgccttt gcaagtttat
    10981 gatatttcag tcta
//
LOCUS       DAZFFI010000143        10985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFFI010000143 DAZFFI010000000
VERSION     DAZFFI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.143"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(174..1856)
                     /gene="glcA"
                     /locus_tag="ACHJ12_004334"
     CDS             complement(174..1856)
                     /gene="glcA"
                     /locus_tag="ACHJ12_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO4992032.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2212..4383)
                     /gene="glcB"
                     /locus_tag="ACHJ12_004335"
     CDS             complement(2212..4383)
                     /gene="glcB"
                     /locus_tag="ACHJ12_004335"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO4992033.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSYLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDELLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4405..4809)
                     /locus_tag="ACHJ12_004336"
     CDS             complement(4405..4809)
                     /locus_tag="ACHJ12_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO4992034.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4814..6037)
                     /gene="glcF"
                     /locus_tag="ACHJ12_004337"
     CDS             complement(4814..6037)
                     /gene="glcF"
                     /locus_tag="ACHJ12_004337"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO4992035.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6048..7100)
                     /gene="glcE"
                     /locus_tag="ACHJ12_004338"
     CDS             complement(6048..7100)
                     /gene="glcE"
                     /locus_tag="ACHJ12_004338"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO4992036.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7100..8599)
                     /gene="glcD"
                     /locus_tag="ACHJ12_004339"
     CDS             complement(7100..8599)
                     /gene="glcD"
                     /locus_tag="ACHJ12_004339"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO4992037.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8850..9614
                     /gene="glcC"
                     /locus_tag="ACHJ12_004340"
     CDS             8850..9614
                     /gene="glcC"
                     /locus_tag="ACHJ12_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO4992038.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9621..10763)
                     /gene="yghO"
                     /locus_tag="ACHJ12_004341"
     CDS             complement(9621..10763)
                     /gene="yghO"
                     /locus_tag="ACHJ12_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO4992039.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSHGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2603 a         3068 c         2752 g         2562 t
ORIGIN      
        1 ttttgcgtga taaaaaaaat agatgttctc acgctcctaa ttatttagca ggttatctga
       61 acacaaaaca ttattgccgc atggataata aagcgagaaa taattttcaa ttaataagac
      121 agaataatgt aaatgaagcc ggatgatatt aacgatcatc cggcattatt gatttacgag
      181 accaacattc cagtaaacac atacgcctgc aacaacgtga taacgccgat aacgctggca
      241 aaaatcagac tgtgcttcac ggtgtagcgg aatagttcag attctcggcc caccatgccc
      301 gtcgcggcgc aggccacggc gatagattgc ggggagatca tcttgccagt tacgccgccg
      361 ctggtatttg ctgccaccag cagagtgtca gagacgttga tttgctgcgc cgtggtcgat
      421 tgcagtgaac caaacagggc gtttgaagaa gtgtccgagc cggtaaggaa tacccccagc
      481 cagccgagga acggtgagaa aaacgggaac atcacgcctg tacctgccag taccagcgcc
      541 aacgtggtgg acatgccgga atagttggtg acgaaggcga acgccagcac catgccaatc
      601 gacaatatcg gccacttcag actaattagc gtttcggcaa agacgccaat ccctttcttg
      661 atgcccacgc cgaggatgaa gatagagata atcgccgcaa taaaaatagc ggtgccgcca
      721 gccgagagag ggtcgaattt aaacaccgca tccattggcg ttggttgggc gacaatgggt
      781 gccgctttca acacttgttg atgcaaatga gggatctgga aattaatcac cagtgaataa
      841 aacgcgccgc ccggagcaaa taacgcttta aacggcttca tggtccagat ggtgaccagc
      901 accgttaaga ttaaaaacgg tgaccacgca cgaatgattt gccccagact atattctgaa
      961 ggcacgggac cgccagaaga tggcttattt accaccatcg cacctgcgga ttgccccatg
     1021 ctgattgccg tttcggtatt tttcggccgc cagactttaa ggaataaagc gagtgagacg
     1081 atactcacca gcgccgaagt aatatccggc agttccggac caatatagtt agaggtaaag
     1141 aactgagtga cagcaaagct tcccccagca accagcgccg ctggccacgt ctctttcacc
     1201 cctttccagc cgtccatcat tgctaccagc cagaacggca caagaaccga caggaacggt
     1261 aactgacgtc ccgccattgc gccaatgtgg aacggatcga ttcccgttac ctgaccggcg
     1321 accagaatcg gcacgcccaa cgcaccaaac gccactggcg cagtgttggc aatcagacac
     1381 agccccgccg cgtataacgg tttgaagccc aggcccacca gcagcgcacc ggtaatcgcc
     1441 accggcgcac caaagccagc cgctccttcc agtaacgcac caaaggagaa accaatcagt
     1501 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     1561 tgaccgctgg caacggttaa tttatagagg aacaccgccg cgacaataat ccacgcaata
     1621 ggccataatc cataaataaa gccatagccc gcagcagcaa aagccatatc aatcggcatt
     1681 ttaaaggcga atattgcaat caggatagat aatataaggg ttattgctcc agcgacatgt
     1741 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     1801 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     1861 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     1921 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     1981 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2041 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2101 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2161 ttactatcat agccccgaca ataaaacttg ccggggcttt ttttgacgct attaatgact
     2221 ttctttttcg cgtaaacgcc aggcgtgtaa taacggttcg gtatagccgt ttggctgttt
     2281 cacgccgagg aagattaaat cgctggcagc tttaaaagca cacgagttag cgaaattccc
     2341 cgccatcgga cgataagccg gatcgccagc gttttgctga tcaaccactt tcgccatatt
     2401 ctccagcgac gcctgcacct gttctttggt cagaataccg tgacgtaacc agttggcgat
     2461 atgctggctg gagatacgca gcgttgcgcg gtcttccatc agcgccacat tgtgaatatc
     2521 cggaactttt gaacaaccaa tcccctgctc cacccagcgc accacgtacc ccagaatccc
     2581 ctgcacgttg ttatccagct cttgttggat ctcttgcgcc gaccagttag cgttttcagc
     2641 aaccggaata gtcagcagct cgtccagcag cggttcaaat tcagcattga actcggtctg
     2701 ggcaatgttg gcttgcacgc tctgtacgtt ggtttggtgg tagtgcagcg catggagcgt
     2761 agcagcggtt ggtgacggaa cccaggctgt gtttgccccg gcacgcagtt ggtcgccctt
     2821 ctggctgtac atgtctgcca tcaggtccgg cattgcccac atgcctttac caatttgcgc
     2881 tttaccgcgc agcccacaga acagaccgga aagcacgtta ttacgctcgt aggctttgat
     2941 ccacggcgtc gatttcatct ggtttttacg cagcatcggg ccagcttcca tcaccgaatg
     3001 catttcatcg ccggtacggt cgaggaaacc ggtattgatg aacgccacgc gattgcgcgc
     3061 ctgagcgata cagctacgca agttcagcga ggtccgacgt tcttcatcca taatgcccat
     3121 tttcagggta ttcggtgcca taccgagcat tgtctcaatg cgggtaaaca gtttgttggc
     3181 gaacgccact tcctgcggac cgtgcatttt cggtttcaca atatagacgc tgccagtgcg
     3241 cgagtttttc tgcactttta aatcatagag ggcgatcgcg ccagtcatga cgccatcaag
     3301 aatgccttcc gggatttcat tgccttcgct gtcccaaatc acaggaatgg tcatcaaatg
     3361 acccacgttg cggataaaca gcagcgagcg tccgtgcaga gaaatttcag agccatcggc
     3421 ggcggtgtaa tgacgatcgt cattcagttt acgcacgatt tgtcgaccgt ttttctccat
     3481 tttctcttgc agagtcccct gcatcaggcc cagcagattg cggtacagca ggattttatc
     3541 ttccgcatca accgccgcga ccgaatcttc gcagtcgaga atggtactga tagcagcttc
     3601 gacgataaca tcgttgatgt gcgccggatc gtctttgcca atccgcccgt tggcatcaat
     3661 ttgcagttca atatgcaggc cgttattttt cagcaaaatg caggtcagcg cagcggcatc
     3721 gccacggtaa ccgacaaact gcgccggggt acgtaacgtg gtttctttac catttttcaa
     3781 ctggatgcgt aattgtttat cgaccacctt aaacgccacc acatcctgat agctgccgtt
     3841 ttccagcggt agagattcat cgaggaaacg ccgaacccag gcgataacct gctcaccgcg
     3901 ttgcggatcg tagccgctga ccaccgcccc ttcctgcggg atgatgtcgc tgccgtataa
     3961 cgcatcgtac agtgagcccc agcgagcgtt cgccgcgttc agcgcgtagc gggcgttcat
     4021 tgccggaacc accagctgtg gccccgcctg actggtgatt tcgctgtcaa tgcccgtggt
     4081 ttccaccgtc acgcgctccg gttgcggcac caggtagccc agttcacgca ggtaagattt
     4141 ataggccgct ttatctttta ccggccccgg attgctgcga tgccactcat caagcgccgc
     4201 ctgaatgcga tcgcgttctg ccagcaactg acgattttct ggtgccagat cgtgaacgat
     4261 ctcatcaaaa ttgcgccaga acgccgcagc gtccagccct gttcccggta aaacttcttc
     4321 atccacaaaa cgtttaaaat tggcgtcaat gcgtaaacgg ccctgggtta tggtttgact
     4381 cattgtttat ctcctcgttt tcgcttattt cgccaacacc gctgctgccg ctttcgcgac
     4441 ctgcgcatcc tgtgctccgg ttaaaccaga aacgcccacg gcaccaataa tttgcccatc
     4501 aacaacaacc ggtacgccgc cttccagcga cgttaataac ggcgcagtca cgaacgcggt
     4561 acgtccgttg ttcaccatct cttcatagcc cttagtttca cgacgcccca gcgcggcggt
     4621 acgcgctttc tcctgggaga tgtaagccgc aatcggcgcg caatcgtcca tgcgacttaa
     4681 cgccagcaga tgaccgccgt catcggcaac agcaatggaa accgaccagt tatttttctg
     4741 cgcctcttcc tgacctgcgg caataattgc actcgccatt tgctggctaa gaatgacttt
     4801 agttttcatt ttgttattcc ttttcaaggg cttgttctac aatttcaatc cagtgacgca
     4861 cagaggtacg accggcgctc gccagatgcg tctggcaacc aatgttggcg gtgacgatca
     4921 tttccggttt gccgctttcc agcgcattca ttttgttatc ccgcagctgg cgtgccagat
     4981 cgggatgcgt taacgcatat gttcccgctg aaccgcagca cagatggctg tcgggaacgt
     5041 ccgttaaggt aaatccaaga cgaagcaaca ctttttccac ttcgccgttc agcttttgcg
     5101 catgttgtag ggtacacggg cagtggaagg ccagtttttt atcgccgcga actgccagtt
     5161 tttccagcgg ttcctcgcgc agaagctcga ctaaatcgac cgccagttca ctgacttgac
     5221 gcgctttatc ggcatataac gcatcgtttt tcagcatctg cccgtactct ttgacaaacg
     5281 cgccgcaacc gctggcggtt tgcaaaattg cctcagcacc tgcttcaatc gcgggccacc
     5341 aggcatcaat attattgcgc gcccgtgcca gccctttctc ctgcgcatta agatgatagt
     5401 ccaccgcgcc acaacagcct gcttcgttag ctgacatgac gctgatcccc agacgatcca
     5461 gcactcgcgc agttgccgcg ttggtgttgg gcgaaagcgt aggctgggcg cagccttcca
     5521 gcattaaaac ccgacgctta tggcgcagcg gcggacgcgg tttagctttc accgtttcag
     5581 caggcagttt tgctctgacc tgttccggta aaaacggtcg cagcaccagc cctacctgcg
     5641 tcagcgcacg gaagaccgcc ggacgcggca ctacctggcg caatccttcg cgcagcattc
     5701 gctccggcag tgggcgtttc actttctgct cgacaatatc acgcccgata tccagcaaat
     5761 tgtgatagcg cacaccagaa ggacaggtgg tttcacaatt gcggcaagtg aggcagcgat
     5821 cgagatgctc ctgtgtttta agcgtgactt cgttgccttc cagcacctgt ttaatcagat
     5881 agatgcgccc gcgcggcccg tccagttcat cgcccagaag ctgataggtt gggcaggttg
     5941 cggtacaaaa tccgcagtga acacaggcgc gcaggatgct gtcggcttcc agcgcgcgcg
     6001 cgttctgccg catctcttca gttaattggg tttgcatagc ctgctcctca aagttccgcg
     6061 tacatgcgac cggggttaaa cacgccgcaa gggtcgagct gctgtttaag ctgctggtga
     6121 tagcggaata aaggagccga tagcggggca aagccaccat ctccggcact aaagcgggtc
     6181 gcatgaccgc cagcgttgcg ggcgatgcga tggatttgat tgtcctcggc tgtcgatttc
     6241 agccagcgta acgctccgcc ccagtcgatc agttgctcgc cgggtaaatc catcatcggc
     6301 gcatcgctgg gtaatgaaat gcgccataag gtacctggta acgagaagaa cggcagttgt
     6361 tgttcacgca attgctgcca gaactgaccc gcaacctctt cgccccccag cagttcacgc
     6421 gccgctttta ccgatccttc gccgccctca aggcggatcc acaacgcatt gtcgaagtaa
     6481 cataagccac taatgggtag cggctggagt tgccactggg cgatttcgtt aatggcttct
     6541 tgcaggctga tttcccgacg caggctcagg gaggcgcgcg gtcgcggtaa cactttcatc
     6601 gagatttcag tgagcacgcc aagacaaccg tagcttccgg ccattaaccg tgagagatcg
     6661 tatccggcaa cgtttttcat cacttcgcca ccaaaacgca gatgttttcc agcgccggta
     6721 atgatgcgcg tgccgaggac aaaatcgcgg accgaaccgc tccacgggcg acgcggcccc
     6781 gccagcccgc aggcgaccat cccgccccag gtggcttctt caccataatg cggcggctcg
     6841 caggggagca tttgcccggc gctctccagc gccgcttcaa tcgccaccag cggcgttccg
     6901 gcacgcgcgg ttatcaccag ctcggtcggg tcgtaattaa caatgccgcg atgacaacgc
     6961 acatccagcg tttgcccggt gacagggcga cctaaaaagg ctttgctatt gctgccctga
     7021 atcaccagcg gcgttttatc gctaattgcc tgattcacct gctccagcag cgcctggctg
     7081 taatcacact cgcgtagcat cagaaacgct ccagttcagg gaaaggtaaa tgaccgtgat
     7141 gcacatgcat ggcaccaaat tcagcacagc ggtgtagcgt gggaatgttt ttcccagggt
     7201 tcagcaaacc atcggggtca aacgccgcct tgaccgcatg gaaggtcgtg atttcatcgc
     7261 tgttgaactg ggcgcacatt tgattgattt tttctcgccc gatgccatgt tccccactga
     7321 tgctgccgcc aacttcaacg cagagttcga ggatcttccc ccccagctct tccgcgcggg
     7381 caaattcacc gggttcgttg gcatcgaaaa ggattaacgg gtgcatgttg ccatctccgg
     7441 catgaaagac gttggcaaca cgtaaatcat attgctgcga taaacgggca atgccttcca
     7501 gtacgccagg cagggcgcga cgcgggatgg tgccatccat gcagtagtaa tccggggaga
     7561 tacgtcctac cgccgggaac gcatttttgc gaccggccca gaaacgtacg cgctctgctt
     7621 cgtcctgtgc cagacggacg tcagtcgcgc ccgctttcaa caagatgtcg ttaacccgct
     7681 cgcagtcttc ctgtacgtca gactccacgc cgtccagctc gcataacaaa attgcttcgg
     7741 cgtcgacggg ataaccggca tgaataaaat cttccgccgc gcggatcgac aggttatcca
     7801 tcatctccag cccgccgggg ataatgccat tggcgatgat gtcaccaact gcaagtccgg
     7861 ctttttctac cgagtcaaag ctggctaaca gaacccgcgc cacgggcggc ttcggcagca
     7921 gttttaccgt cacttcggtg gtcacgccga gcataccttc cgatccggtg aacagcgcca
     7981 gcaggtcaaa accaggtgaa tccagcgcgt ccgatcccag cgtcagcgcc tcgccgtcca
     8041 gcgtttgcac ttcaattttc agcaggttat gtacggtcag accatatttc aggcagtgga
     8101 cgccgccggc attttctgcc acgttgccgc caatggaaca ggcgatttgt gaggaagggt
     8161 ccggtgcgta gtagagatta tgcggtgcaa cggcctggga gatcgccagg ttacgcacgc
     8221 ctggctgcac gcgcgcgcgg cgaccaacgg ggttaatgtc gaggatctct ttaaagcgcg
     8281 ccatcaccaa caacacacct ttttccagcg gcagcgcgcc accagaaagc ccggtgcctg
     8341 caccacgggt caccaccggt acacgcaggc gatggcagac agcaagaatc gctgtcacct
     8401 gttccatttg cttaggcaga acaaccagta atggacgcgt gcgatacgcg ctcaacccgt
     8461 cacactcgta aggaatgatc tcctcatcgg tatgcaggat ctcaagtcca gggacatgct
     8521 cacgcagtgc catcagtacc gatgtgcggt cgacatcggg taaagcgcca tcaagacgct
     8581 cttcgtacaa gatgctcatg agtaggcttc gctttgttgt gttgtgtggc agctgatttt
     8641 tgcgcgctgc ttctgtgaac agttattaag cgggcttttc gttttcgtct atctctttag
     8701 ctaccggtca gaccattttt tttccagctc tgtgaccttg ccttggttaa ctcaatgtta
     8761 aattgatgta acataatcac ttacgtgatg tgcgtgtttt gcgagttaag aacagaaaaa
     8821 ttggtcctac ctgtgcacga ggtccgggaa tgaaagatga acgtcgccct atttgcgaag
     8881 tggttgcaga gagtatcgaa cggttaatta tcgacggcgt actgaaggtc ggtcagccac
     8941 ttccctcgga acgtcgactg tgtgaaaagc tcggcttctc acgctccgca ctgcgtgaag
     9001 ggctgaccgt gctgcgcggg cgcgggatta ttgagacggc gcagggtcgc gattctcgtg
     9061 tcgcacggct taatcgggtg caggacacca gcccgctgat ccatctgttc agtacgcagc
     9121 cgcgaacgct gtacgatctg ctcgacgttc gcgcattact ggagggcgaa tcggcaaggc
     9181 tggcggcaac gctgggaacg caggctgatt ttgttgtgat aacccgctgt tatgaaaaaa
     9241 tgctcgccgc cagtgagaac aacaaagaga tttcgctgat cgaacatgcg cagttggatc
     9301 acgctttcca tctcgccatt tgtcaggctt ctcacaatca ggtgctggtg tttacgctgc
     9361 aatcattgac cgatctgatg tttaattcag tgtttgccag cgtaaataat ctctaccatc
     9421 gaccacagca aaaaaagcag atcgatcgcc agcatgcgag gatctacaac gcggtgttgc
     9481 agcggctgcc gcacgtcgcc cagcgcgcag cacgcgatca tgtgcggacc gtgaaaaaga
     9541 atctccacga tatcgagctg gaaggccacc atttgattcg ctcggcggtg ccgctggaga
     9601 tgaacctgag ttagctggta ttaaatctgc ttttcataca atcggtaacg cttgtacggc
     9661 tccgccccaa tgcgttccag catgttattc atgcctgtat tggtttcgag gatccatgac
     9721 atctccagcg catcgatctt ccggcgggca aacggatcgc gtaaggcttc aatcaataac
     9781 agcgcaatca ccgggccgat gcggctgaac tgatactcgt cgcgcacgcc catcagcggt
     9841 actcgcgcag ttcgcacacc gctgactttc aaacgccaca gcaattttgc ccagccgaag
     9901 ggaaagagcg atccgttcag atcggcaatc gcctcgttga tgttcggcaa gccgacaata
     9961 aacgcgcagg gtgcagaatc aatctcagcg atatagatca tatcgtccgg caccagatat
    10021 ttaagttgat cgcccatggt cgcgaattca tgttcggtaa acggcacaaa tccccagttg
    10081 tgctgccagc cagagttgaa aatctcacgc aggatctgca tttcttcggc aaaccgctga
    10141 cgattgatgc agcgaatggt cacctttttg cgcacctgat ccatcagttt ttttagcgcc
    10201 ggagagaaag tgagatcggt tcgctgcatc caccacgcca gtaaatcaat gcctttgtga
    10261 taacccagtt gttcaatatg cgcggcatac cacggtttgc cgtgtggcat catcgcacag
    10321 ggtggtgtgt caaaaccttc aatcagtaat ccgctttcct gattgatatt caggctgaaa
    10381 ggaccgctga tcttacttgc accatgtgac ttcaaccacg cttccgctgc gccaaacaac
    10441 gcggcaaaaa cctgcggatc atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca
    10501 ccgtaacgct cacggtgcaa ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc
    10561 gctttttttg ccacccacgc ctgccagatg atatggtccg tccccggatt tttcgcagac
    10621 aaatgctcgt tgcgttctat gaataaaggg ggtatccagt ttggatcgtc gggataaagt
    10681 gatgacggaa aagcgataaa tgccttaagg tcatttttat taattacttt ttcaatttta
    10741 atcatcagca gatcgcattc catatctctg tggataacaa ataattaacg gtgaaaaaca
    10801 ggttgcaaca gcaacgaacg acaccttaat aatcaaccca atgaatacaa acaaagaata
    10861 aatagtattt attaatgatt atgataaggt tagagactac acagaagcgc ctcaatgcct
    10921 tttgtcgatt taaggcgacc ggacaaggct tcttcttaat atattcataa attaaatatc
    10981 attca
//
LOCUS       DAZFFI010000144        10963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFFI010000144 DAZFFI010000000
VERSION     DAZFFI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.144"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..919
                     /gene="hlyE"
                     /locus_tag="ACHJ12_004342"
                     /pseudo
     CDS             9..919
                     /gene="hlyE"
                     /locus_tag="ACHJ12_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1126..1572)
                     /gene="ycgN"
                     /locus_tag="ACHJ12_004343"
     CDS             complement(1126..1572)
                     /gene="ycgN"
                     /locus_tag="ACHJ12_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4992040.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1664..2323)
                     /gene="ycgM"
                     /locus_tag="ACHJ12_004344"
     CDS             complement(1664..2323)
                     /gene="ycgM"
                     /locus_tag="ACHJ12_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4992041.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2395..2688)
                     /locus_tag="ACHJ12_004345"
     CDS             complement(2395..2688)
                     /locus_tag="ACHJ12_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4992042.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2930..3331
                     /gene="pliG"
                     /locus_tag="ACHJ12_004346"
     CDS             2930..3331
                     /gene="pliG"
                     /locus_tag="ACHJ12_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4992043.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3451..3819)
                     /gene="ycgJ"
                     /locus_tag="ACHJ12_004347"
     CDS             complement(3451..3819)
                     /gene="ycgJ"
                     /locus_tag="ACHJ12_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4992044.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4339..5034
                     /gene="minC"
                     /locus_tag="ACHJ12_004348"
     CDS             4339..5034
                     /gene="minC"
                     /locus_tag="ACHJ12_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4992045.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5058..5870
                     /gene="minD"
                     /locus_tag="ACHJ12_004349"
     CDS             5058..5870
                     /gene="minD"
                     /locus_tag="ACHJ12_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4992046.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5874..6140
                     /gene="minE"
                     /locus_tag="ACHJ12_004350"
     CDS             5874..6140
                     /gene="minE"
                     /locus_tag="ACHJ12_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4992047.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6512..6856)
                     /locus_tag="ACHJ12_004351"
                     /pseudo
     CDS             complement(6512..6856)
                     /locus_tag="ACHJ12_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6891..7010)
                     /locus_tag="ACHJ12_004352"
     CDS             complement(6891..7010)
                     /locus_tag="ACHJ12_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4992048.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6971..7156)
                     /gene="ymgJ"
                     /locus_tag="ACHJ12_004353"
     CDS             complement(6971..7156)
                     /gene="ymgJ"
                     /locus_tag="ACHJ12_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO4992049.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7257..7430
                     /gene="ymgI"
                     /locus_tag="ACHJ12_004354"
     CDS             7257..7430
                     /gene="ymgI"
                     /locus_tag="ACHJ12_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO4992050.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7432..7776
                     /gene="ymgG"
                     /locus_tag="ACHJ12_004355"
     CDS             7432..7776
                     /gene="ymgG"
                     /locus_tag="ACHJ12_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO4992051.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7780..8115
                     /gene="ymgD"
                     /locus_tag="ACHJ12_004356"
     CDS             7780..8115
                     /gene="ymgD"
                     /locus_tag="ACHJ12_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO4992052.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8172..10493)
                     /locus_tag="ACHJ12_004357"
     CDS             complement(8172..10493)
                     /locus_tag="ACHJ12_004357"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4992053.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYAIWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGNNGYNDTAVMVGLKYKF"
     gene            complement(10610..10819)
                     /locus_tag="ACHJ12_004358"
     CDS             complement(10610..10819)
                     /locus_tag="ACHJ12_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992054.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3074 a         2598 c         2395 g         2896 t
ORIGIN      
        1 gaatgattat gactgaaatc gttgcagata aaacggtaga agtagttaaa aacgcaatcg
       61 aaactgcaga tggagcatta gatctttata ataaatatct cgatcaggtc atcccctggc
      121 agacctttga tgaaaccata aaagagttaa gtcgctttaa acaggagtat tcacaggcag
      181 cctccgtttt agtcggcgat attaaaacct tacttatgga tagccaggat aagtattttg
      241 aagcaaccca aacagtgtat gaatggtgtg gtgttgcgac gcaattgctc gcagcgtata
      301 tttttctatt tgatgagtac aatgagaaga aagcatccgc ccagaaagac attctcatta
      361 aggtactgga tgacggcatc acgaagctga atgaagcgca aaaatccctg ctggtaagct
      421 cacaaagttt caacaacgct tccgggaaac tgctggcgtt agatagccag ttaaccaatg
      481 atttttcaga aaaaagcagc ttttccagtc acaggtagat aaaatcagga aggaagcgta
      541 tgccggtgcc gcagccggtg tcgtcgccgg tccatttgga ttaatcattt cctattctat
      601 tgctgcgggc gtagttgaag ggaaactgat tccagaattg aagaacaagt taaaatctgt
      661 gcagaatttc tttaccaccc tgtctaacac ggttaaacaa gcgaataaag atatcgatgc
      721 cgccaaattg aaattaacca ccgaaatagc cgccatcggg gagataaaaa cggaaactga
      781 aacaaccaga ttctatgttg attatgatga tttaatgctt tctttgctaa aagaagcggc
      841 aaaaaaaatg attaacacct gtaatgagta tcagaaaaga cacggtaaaa agacactctt
      901 tgaggtacct gaagtctgat aagcgattat tctctccatg tactcaaggt ataaggttta
      961 tcacatttaa aaacacagac gttttttaaa gattgagagt tacctgttga taataaggta
     1021 tcgggtaact ctcattatga cagagcgctt tcaaaaacag ggctacgaaa tggcgagcat
     1081 ccagacgtat attaagaaag cccccatcaa catctattaa atttatcact gcgcccagtc
     1141 aggtttattt aagatatgat cctgccagtc aatcacttct gattctttca ctgcgatatg
     1201 acgcacagag atacgttcac catgcattgc cgctttcgaa ccagtaagta gcggatgcca
     1261 cgcaggtaaa tctttacctt ccgccagcaa acgataagcg caggtcattg gcagccattc
     1321 gaatgttggc agattttcac gggttaattt aatgcagtcg ggttcaaact cgaaacgacg
     1381 ttcgtagttc cgacattgac aagttttaat attgagctgg cgacaggcga cgttagtgaa
     1441 gtagatttcg tcggtgtctt catccatcag tttatgcagg caacactgac cgcaaccatc
     1501 acacaacgac tcccattccg catcgctcat ttcgtccagg gttttacttt gccagaaagg
     1561 tacatcgctc atcaggtgtt ccgccattac gttaaaacgc accttataac cagtctggca
     1621 cagcgatgca agttttgccg ccgctttcag gcggcaaaaa gtattacaaa acgcgagttg
     1681 tcaaagaatg cccatcgaaa gtgactgtca gctcatcacc gctttgcaac gggccgacgc
     1741 catcaggcgt gcctgtcaac acaacgtcac cggccttgag ggtaaaaaac ttgctcatat
     1801 aagcgatcaa cggaacgatt ttatggatca tgtccgcagt cgtaccttgc tggcgttgtt
     1861 cgccgtttac gctcaggccc agcgttgtat tttgtggatc gccggtaaat tccgccgcgg
     1921 ggataaaccc ggaaagcgga caagagttat caaacgcttt agccttttcc cacggctgcc
     1981 cggctttctt catttttccc tgaacatcac gcaatgtcag atcaagcgcc acgccgtaac
     2041 cggcaatggc tttgcggaca tgctcttccg ttgcctgacg cagcgtcgcg ccaatcaaca
     2101 ccgccagttc gacttcatga tgaactgaac cgaaatcgga tgggatcgcc aatggctgcc
     2161 gcagatcgca cagtgccgtt tctggtttaa taaacagcac tggctcttcg ggcactgcgc
     2221 tgcccatctc tttaatatgt ttggcatagt tactgccaac acagactact ttactcaccg
     2281 gataatccag cagcgcacct tgccagttgt gatgttgata cataacgttc ccctcacctt
     2341 atcgtggata tgaaaacccg cgccgacaaa gataatgcca ccgccggata tcggttattt
     2401 gttagtgtcg tctgttttct gccccatgac ggaaagatgt tgcttcagca aatcttcggg
     2461 tggtggcggt aactgcaaat agtaaccttg ctcggttaat gccagtttaa ccttttcaat
     2521 atcagcattg accagtttct tgcgcccatc cagcggcaga atcatcgcta actgaggctg
     2581 accaaaacct ttcatcagtt cctcaggaac acgcgaaaaa tcgtcttttt tttcgacata
     2641 taaataggtc tggtcacgct tgctgcttcg ataaatcaca caaaacatac ttttactctt
     2701 taagatgcca ggttttggca taagctaaca tcaatgaaac ccgcgccaca agccagccag
     2761 ctgtagattt ctgcggtttg ttgagtccat gcccctttat aagtaatccc cccggcaaaa
     2821 aatgccgcgc cgggatttta ccgaacgcag aaaagccact ggaattctcc cgtaagggat
     2881 tgttatatta taacagttca tcgtactcat tctgaacagg agactaccaa tgaaaatcaa
     2941 gagcatcagt aaggctgtat tgctgcttgc actgctaacc tccacgagct ttgctgcggg
     3001 taagaatgtc aatgttgagt tcagcaaagg ccatagcagc gctcagtact ccggcgaaat
     3061 aaagggatac gattacgata catatacctt ctatgccaaa aaaggtcaga aagtgcatgt
     3121 gagtatctct aatgaaggcg ctgatactta cctcttcggg ccaggaatcg atgattcagt
     3181 tgatttatcc agatattcac cagaactcga tagtcacgga caatactcac tccctgcttc
     3241 tggcaaatat gagctgcgag tgctgcaaac ccgaaacgac gcccgtaaaa acaaaactaa
     3301 aaaatataac gtcgatattc aaattaagta aatgtcaacc gacggggact ctccccgtca
     3361 taaacgttaa cgccccactc ctctctgtcg ctcaagaggt taatgacgaa tagcagacaa
     3421 tgccgaatag aacataatgt aaacaaggca tcactcacga cacataaata acatctttgt
     3481 ggtgatttga ttgatttttc cactccgctt accatccaca ccaaaataac gatcatcacg
     3541 acataatttt tctttaacat cgcaaaatac accgttagcg aatgtaaatt cgctgggatc
     3601 gtagccttgt aatgattgta agttttcagc ggctttttcg ccgagatatt tccttgttaa
     3661 ctctggggaa atgccatctg agttcacaca aatatacggg tcgcaaacaa cgccgcacgc
     3721 cggagattga agtccgccag cgtcatgtcc agacgccatt gatgagaaca tcatccccac
     3781 acctgacaat ccgatataaa aaatacgcat catattcata gcacatcctg ataatccaac
     3841 cgtacaacac ttatgctcat ttcatcatca atccgtaatc aaaaccgctg acctggacga
     3901 gttacgatga cctgacagaa gtatcagacg tcttctgcgt tttctatgat agagcaattc
     3961 tgtggcggat attaagataa cgggtatctg ttctttaatg atgccgcaaa aaatggaaag
     4021 ttaagaaagc ctggcgtggt gaaaaaatac acttgcctca atactggata tttaaacagg
     4081 tctggagatg ctgtcacgat tagggtgcgg aaaaaggaag cgtaaataag aacaatcaaa
     4141 agtacgacgg caatgggttg attgacaagg gtatttttta agctatgaat cagcgccatt
     4201 tatcacagaa tagactttta ctctgaataa atgggagggt gacttgcctc aatataatcc
     4261 agactataac atgccttata gtcttcggaa catcatcgcg cgctggcgat gattaatagc
     4321 taattgagta aggccaggat gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact
     4381 ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctggaagac
     4441 aaaatcgctc aggcccccgc atttttaaaa catgcccccg ttgtactcaa cgtcagtgca
     4501 ctggaagacc cggtaaactg gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg
     4561 gttattggcg taagtggctg caaagatgcg caacttaaag ccgaaattga aaagatgggg
     4621 ctgcctatcc tgacggaagg aaaggaaaaa gcgccgcgtc cagctcccgc accacaggct
     4681 ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc
     4741 ggtcagcgta tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg
     4801 gccgaattga ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctt
     4861 gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaactg
     4921 gtgtccatcg caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa
     4981 gcggcgcgac tgcagttagt cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt
     5041 tttaacaagg aatttctatg gcacgcatta ttgttgttac ttcgggcaaa gggggtgttg
     5101 gtaagacaac ctccagcgcg gccatcgcca ctggtttggc ccagaaggga aagaaaactg
     5161 tcgtgataga ttttgatatc ggcctgcgta atctcgacct gattatgggt tgtgaacgcc
     5221 gggtcgttta cgatttcgtc aacgtcattc agggcgatgc aacgctaaat caggcgttaa
     5281 ttaaagataa gcgtactgaa aatctctata ttcttccggc atcgcaaaca cgcgataaag
     5341 atgccctcac ccgtgaaggg gtcgccaaag ttcttgatga tctgaaagcg atggattttg
     5401 aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt
     5461 ttgcagacga agccattatt accaccaacc cggaagtctc ctcagtacgc gactctgacc
     5521 gtattttagg cattctggcg tcgaaatcac gccgcgcaga aaatggcgaa gagcctatta
     5581 aagagcacct gctgttaacg cgctataacc caggccgcgt aagcagaggt gacatgctga
     5641 gcatggaaga tgtgctggag atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc
     5701 aatcagtatt gcgcgcctct aaccagggtg aaccggtcat tctcgacatt aacgccgatg
     5761 cgggtaaagc ctacgcagat accgtagaac gtctgttggg agaagaacgt cctttccgct
     5821 tcattgaaga agagaagaaa ggcttcctca aacgcttgtt cggaggataa gttatggcat
     5881 tactcgattt ctttctctcg cggaagaaaa acacagccaa cattgcaaaa gaacggctgc
     5941 agattattgt tgctgaacgc cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc
     6001 gtaaagatat tcttgaggtc atttgtaaat acgtacaaat tgatcctgag atggtaaccg
     6061 tacagcttga gcaaaaagat ggcgatattt ctattcttga gctgaacgtg accttaccgg
     6121 aagcagaaga gctgaaataa gcccgctgta aaaacgcatt tatcttcaag gcagagtcat
     6181 ttctgccttg agtttttcat ccctctcatc cacgttgtgg taaagcggcg agtattcttg
     6241 ctgatactcc tcattgctat ttccacctcc ccctttacac cttaaggctg ttcccacgca
     6301 cagaacaaaa tgtttatgcc tggttgagta aataaccctg ttgttagtat ggatatactg
     6361 ggagatgata accccgcaat ttcattattc attgttcccc cataggatga ggtttcccgc
     6421 cgtaactggc gggctttttt tgccccagaa ttttctcttt caacatcctg taaacgaaaa
     6481 ctgcgccgaa gcgcagttta atcgttgatg gtcagaacga ccagttcaca ccagccaccg
     6541 cgttccacgg ggactccaca ccggcaccat ggctataccc caccccgaaa tgcccgctta
     6601 acgtactgct gaatgaggct ttaatacctg cctggtatat tccacgtctg cccgacaaat
     6661 cattgacaaa attaccgtca ctattcactt tcacccgatt atcatcgaca aattctttgc
     6721 gcacagccgc cttcaggcag ggttcaattt ccataccgtt ccccagacgc atgttgtaac
     6781 tcagcgttgc acccagttca cgatatatac tgcgggtatc gactgatttc gattccatgc
     6841 cattggataa atgatagtag cgtctaagtg acctaagctc tgctgcaaca tcagcccgca
     6901 ggcaccagac caggggattc atcctgaaga gacagcgcag gtgtattgtg ttcaccgctc
     6961 accaaagaca tcatgatgaa atgatgatat cccgcataag aaagaggcat tttttacacg
     7021 cagtgcgctg aagtgtagtt ggataaaaaa tcactctttt cagataatac tgtcgtacgc
     7081 ttatctttcg acaatgaggc cgtcggcaaa ataaaatgac taatgtaatc gttgctgatg
     7141 aatatcttct gctcacataa aaatcacaca ataatattga taaatcagat tatcttacct
     7201 ttacaacatt gcgttaacag tcaatgacac ttcttgaata aaaatattgt caatcaatga
     7261 gttattcctc atttaagatt attctcattc acgtaaaaaa cataattcct atcatcacag
     7321 ccacgctcat tttgaattat ctgaataata gcgagcgttc tttagttaag cagatcttaa
     7381 ttgaagacga aattattgtg tgtgcaactt acttaatacc ggacatctaa gttgaagaaa
     7441 aaaatacttg ccttcgggct tatctcagca ttgttctgct ccacgccagc gatggcggat
     7501 atgaatcgca ccacgaaagg tgcattgcta ggcgcaggcg ttggtttact tactggcaat
     7561 ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg gtgttggtgc agtaacagaa
     7621 aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag taggtgccgc cgttggtgct
     7681 gtcactggcg ttctgaccgg caatggcctc gaaggggcga ttaaaggtgc cgtgattggc
     7741 ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa tgatgatgaa aaaatttgct
     7801 ttactggcgg gcctgtttgt tttcgcacca atgacctggg cgcaggacta caatattaaa
     7861 aacggtttgc cgtcagaaac ctatattact tgcgcagaag ccaacgaaat ggcaaaaact
     7921 gacagcgcac aggttgcaga aattgttgcg gtaatgggta atgccagcgt tgccagccgt
     7981 gatttaaaaa ttgagcaatc tccggaactg agcgccaaag tggtagaaaa actgaatcag
     8041 gtttgcgcta aagacccgca aatgctgttg atcacggcaa ttgatgacac catgcgtgct
     8101 atcggcaaaa aataatattt catatgtacc gcgtcgatga atacgacgcg gttttttagc
     8161 taacggcggg atcagaactt atatttcagg cccaccatca ctgcggtgtc attgtagcca
     8221 ttattaccca gctgcacgcc cacattcccc cagacgctgg ccgctggatt caggttgcca
     8281 tttacgccaa gtttaatttc ccccaaatta ctcgccccat cttgttcgac tttcacaccg
     8341 ttcattgaga cagcaaaatc tttactgtta tggatccagt tcatttcaac aaaaggttca
     8401 aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac gaataaaggt gcggaaacca
     8461 agtcgggtct ggatgttgtt atttgcgtca ttctcaatac gtgagccatt ggcttcggtg
     8521 tgcgcattat gatcgacgcc catccagata gcctgtgcct gtggctggaa tatccacgtg
     8581 tatttcgccg cttccagacc aaaggattta ttcagggcaa agccgtaacc cgcttccagc
     8641 gagacggttg cgcctttcgc actataggat tcataggcta attcatcccc tttcaccgag
     8701 tttttaaacc agctatattg cgcccaggcg tcaatgtatg cgcctttttt actgatgtca
     8761 tcggcaaacc aaatggcata cagccctgcg caatagcctc tgacactccc tttcgaacga
     8821 taatccgaca cgctggaatg agttgagttg tagtcgcggg cataaccagc catcactccc
     8881 aaacgccaac tgtcactgtc ggtaaaacca ccggttaaca gctcggcccc taactgcgag
     8941 acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg
     9001 ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt cagttacagg atcgatgtag
     9061 cgcatttcac ccgcacggtc gttcagacgc ataacaaaaa gagtgttagc ggctgccagg
     9121 ttggcgacgt aacttccagc ttcgaggcgc aggacggggg taggcgcaac agtaggcgct
     9181 gaacttcctc cggagtttgt ttcaccgccg ctcgttgcct ggaagttagt cagataccag
     9241 tttttattgg tcgcttcggt attacctcgg gttaatgaat attcgtaggc accggcgaaa
     9301 atcctcgaat ccttaataaa ttccccattt gattccccct caacgctgat aatctctatc
     9361 ccatttaacg tgtaagctcc gctgcctcct gcattggtaa ctttaactcg ggttgttcca
     9421 gaggtatttc cattaataac gactttatcc gtagctgaat tatcatcacc caattcagtt
     9481 ctgagatgca atacgccatt attaccaacg taattttcag ttattgttaa tcttgttggt
     9541 tcgacatccc gtccatctgc ccgggaaata tagaccgtac tattatcaac cgtgaggttg
     9601 ctcaccgttg agtcagcttt aatatcccag gtactattat ctgaaagcga cagataggta
     9661 tgagtgttaa catctgttga aatattagct gaacccgtta tttttgaatt agttgcatcg
     9721 acggtcaaat gacctttaat attattgata aaaacatctg cagccgatga tacctcacta
     9781 tccgtaatgg tcacgttact cgtctcattt ttatggtaga ggatttcatt tgctgaccgt
     9841 agtgagctac cagataaaat atctaccgtt gcttcctgac catttatgga agactccagc
     9901 acaattccag acccctgatg cgcgactgca gatgtatttt tcagcgtcac cgtgccgcca
     9961 ttataaattt tagcagtcgc ggcgtcagga cccgttgtat aaatttctcc ctggttaaat
    10021 gaaactgtcc cctggcgggc agcgatggca tgagcattgt ccccatgcgt ggtgatatca
    10081 acatcggtta atgtaacgct ggcattggta taatcaagcg tgtcaccagc cttgacgaca
    10141 gtgccattag cgtacgcggc ataactttct gttccgtggg ttgtgatctc cccttttgcc
    10201 atatccagtt ttgctatggc attattttca ttacttcctg tatcggtagt actttcttca
    10261 tcaagagaag atacacacgc acgcgcgctt ccgggagatg cacacgcaag aacgccgact
    10321 gcgtgattac ccgcagtaac aataaatgta tcatctccgt cagaaaaaga gggggtaacc
    10381 gaaccgtcag agttgactct tattatggtg tcagtattca ggtttccact cgtttgactt
    10441 agcaaaccaa ttgagtaatc ccccgttgtg acaatcgtgt ttttaccctg caaaataaca
    10501 gcaccgccaa attcacttac gattgcgcta gctgttaatg ctgtgcttag aggactattg
    10561 tcagaaggat ataaaccgcc cgttgttaca atgacgccat tgttaatggt taacttgcta
    10621 ccctctcctg caacggatac ccgtaacttc cattacccgt tgttataact agcgcattat
    10681 caatatcttt tacttttcca tcttttattg ccacagcatg tttatattca tcgtgctgaa
    10741 taaactgttc agcatatgca ttacccaccg ccagagcaat aagtcccaaa ctaaacccgg
    10801 ggagtttttt cagtttcata gattaaccat ccattcattg gattaacaaa cattcctggt
    10861 caaataacct atgagctcag attaccaaaa cggagggtaa agacaaaaac aaaagcacaa
    10921 aaaatttaaa tagataaaca gcgacaatat ataaatgata aat
//
LOCUS       DAZFFI010000145        10900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFFI010000145 DAZFFI010000000
VERSION     DAZFFI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.145"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHJ12_004359"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHJ12_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4992055.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHJ12_004360"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHJ12_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4992056.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHJ12_004361"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHJ12_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4992057.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQKEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHJ12_004362"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHJ12_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4992058.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHJ12_004363"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHJ12_004363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4992059.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHJ12_004364"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHJ12_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4992060.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHJ12_004365"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHJ12_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO4992061.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHJ12_004366"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHJ12_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4992062.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHJ12_004367"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHJ12_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO4992063.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2725 a         2849 c         2690 g         2636 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc gactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatatt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggcagtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggagc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaaagaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgcca atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtaca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtgcgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttgaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggctgggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaacgt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttcca caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaataccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgttc acccgcctgc accagtaggc
     8221 ctcgttgcgt taatgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcatc
     9061 ctggcgcgaa atgactgcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacgc atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
     9361 attcagcgca tcttgttgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg
     9601 caaagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactc tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaattg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcgtcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttgttact tttatttagc
//
LOCUS       DAZFFI010000146        10724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFFI010000146 DAZFFI010000000
VERSION     DAZFFI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.146"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..862
                     /gene="yghS"
                     /locus_tag="ACHJ12_004368"
     CDS             149..862
                     /gene="yghS"
                     /locus_tag="ACHJ12_004368"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4992064.1"
                     /translation="MNQRDMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPA
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            894..1652
                     /locus_tag="ACHJ12_004369"
     CDS             894..1652
                     /locus_tag="ACHJ12_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992065.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1698..3047
                     /locus_tag="ACHJ12_004370"
     CDS             1698..3047
                     /locus_tag="ACHJ12_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO4992066.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPVGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3047..3871
                     /locus_tag="ACHJ12_004371"
     CDS             3047..3871
                     /locus_tag="ACHJ12_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992067.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3883..4443
                     /locus_tag="ACHJ12_004372"
     CDS             3883..4443
                     /locus_tag="ACHJ12_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4992068.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4474..5544
                     /gene="lptF"
                     /locus_tag="ACHJ12_004373"
     CDS             4474..5544
                     /gene="lptF"
                     /locus_tag="ACHJ12_004373"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4992069.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5541..6620
                     /gene="lptG"
                     /locus_tag="ACHJ12_004374"
     CDS             5541..6620
                     /gene="lptG"
                     /locus_tag="ACHJ12_004374"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4992070.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVNPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSVRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(6655..7827)
                     /locus_tag="ACHJ12_004375"
     CDS             complement(6655..7827)
                     /locus_tag="ACHJ12_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4992071.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7827..8075)
                     /locus_tag="ACHJ12_004376"
     CDS             complement(7827..8075)
                     /locus_tag="ACHJ12_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4992072.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8107..9021)
                     /locus_tag="ACHJ12_004377"
     CDS             complement(8107..9021)
                     /locus_tag="ACHJ12_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4992073.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9018..>10724)
                     /locus_tag="ACHJ12_004378"
     CDS             complement(9018..>10724)
                     /locus_tag="ACHJ12_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO4992074.1"
                     /translation="FTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEY
                     QTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGV
                     GQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADV
                     ALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLP
                     FYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELC
                     QRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAEN
                     ALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRN
                     EAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTG
                     RIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRG
                     QLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQES
                     LA"
BASE COUNT         2577 a         2673 c         2797 g         2677 t
ORIGIN      
        1 catccaaaat atttatttgg ttaatatgtt tttatgaaag cgtaattcag gtcaatgtca
       61 caattaacca ttgtcacaat aaggttgaac ggatattctg agcacggacc acgtaatatt
      121 caatacatta tttactgccg ggtttttcgt gaatcaacgt gatatgtcaa taattaattc
      181 cacgccagtg cgtgttattg ccattgtagg atgtgatggt tcaggtaaat cgaccctcac
      241 ggctagcctg gtaaatgaac tggcagcaag aatgccagca gaacacattt atctcgggca
      301 atcgtccggg cgaattggcg aatggatttc acagctccct gttattggcg caccttttgg
      361 gcgttatttg cgaagtaaag cggcacatgt gcacgaaaag ccctcaacac cgcctggcaa
      421 tattactgct ctggttatct atctgctttc ctgctggcgg gcgtacaagt ttcgcaaaat
      481 gttgtgtaaa agccagcaag gctttctgct catcaccgac cgctacccgc aggttgaagt
      541 gccgggtttt cgctttgatg gcccgcaatt ggcaaaaacc acgggcggta acggttggat
      601 aaaaatgtta aggcagcgcg agctgaagct gtaccaatgg atggcatctt atttgcccgt
      661 attgttgatt cgtcttggca ttgatgaaca aaccgcgttt gcgcgtaaac ctgaccacca
      721 actggcagcg ttacaggaaa aaatcgccgt tacgccgcaa ctgacattca atggcgcaaa
      781 gatccttgaa ctggatgggc gacaccccgc cgatgaaatt ctgcaagcgt cactacgcgc
      841 aattcacgcc gcgctttcct gatcaccaaa atggataggg gatgatttaa cgaatggatg
      901 cactacaaac tcaaactgtt aatagtacaa ccgcgccgca gcccaactac attccggggc
      961 tgattgcggt ggtcgggtgt gatggcaccg gtaaatccac actgaccacc gacctggtga
     1021 aatcgctgca acaacactgg caaaccgagc ggcgctatct ggggctgctc tccggcgaag
     1081 acggcgacaa aatcaaacga ttgccgttgg ttggcgtctg gctggaacgg cgactggcgg
     1141 ccaaatcctc gaaaacccaa agcatgaaaa ccaaatctcc ggcgctatgg gcggcggtga
     1201 ttatgtactg cttctcgctg cgaagaatgg cgaatctacg caaggttcag cgactggcgc
     1261 aaagtggcgt tctggtggtc agcgatcgct tcccgcaggc tgaaatttcg ggcttttatt
     1321 atgatggacc ggggattggc gtcgaacgtg cgaccgggaa aatcagcatg tttctggcgc
     1381 agcgcgaacg gcgtttatac caacaaatgg cgcaatatcg cccggaatta attattcgcc
     1441 tgggcattga tattgagact gccatctccc gcaagcctga ccatgactat gccgagctgc
     1501 aggacaaaat cggcgtcatg tcgacgattg gctataacgg cacaaaaatt cttgagattg
     1561 attccagagc gccctacagc gaagtgctgg aacaggcaca gaaggcggtt tctctggttg
     1621 ccatcgtttc tgaccgccga agtttaactt aaggctggcg ggatactttt tactatcaac
     1681 gataaggtca ggctgaattg gcgggtttta acatcaaaca ttggtttgca gatggcgcgt
     1741 ttcgcaccat tattcgcaat agcgcctggt taggctccag taatgtcgtg agcgccttgc
     1801 tgggtctgtt ggcgctctcg tgtgccggta aagggatgac gcccgccatg tttggcgtac
     1861 tggtgattgt gcaatcgtac gccaagtcga tcagcgattt tattaagttt cagacatggc
     1921 aactggtggt tcagtacgga acgccagcct tgaccaacaa taatccgcag caatttcgca
     1981 atgtcgtctc attttccttc tcgctggata tcgtcagcgg cgcggtggcg attgtcggcg
     2041 gcattgcctt actgccattc ctttcccatt cattaggtct ggatgaccaa agcttttggc
     2101 tggcggcgct gtattgcacg ctcattcctt caatggcttc ctccacgccg accggcattc
     2161 tgcgtgcggt agatcgcttc gatttaattg ctgtacagca ggcgacgaaa ccttttctgc
     2221 gcgcagcggg gagcgtcgta gcctggtatt ttgactttgg ttttgcgggt tttgttattg
     2281 cctggtacgt gtcgaatctg gttggcggca ccatgtactg gtggtttgca gcgcgcgaat
     2341 tacgccgcag aaatatccat aatgccttca aattgaatct gtttgagtct gcccgacaca
     2401 tcaaaggcgc gtggagtttt gtctggtcaa ccaacattgc ccactccatc tggtcggcgc
     2461 gtaactcgtg cagcacagtg ctggtgggga tcgtgttagg acccgctgcc gccgggttat
     2521 ttaaaatcgc catgacattc ttcgacgccg ccggaacgcc agtgggtttg ctgggtaaaa
     2581 gtttttaccc agaggtgatg cgtttagatc cgcgcaccac cagaccgtgg ttgttaggtg
     2641 tgaagtccgg tttactggcg ggcggaatcg gtattctggt ggcgctcgcg gtgtttatcg
     2701 tcggcaagcc gctcatttcg ctggtgtttg gcgttaagta tctcgaagcg tatgacctga
     2761 ttcaggtgat gctgggcgca attgtgattt cgatgctggg cttccctcag gagtcattgc
     2821 tattgatggc gggaaaacag cgcgcttttc tcgtggcgca aaccatcgcc tcaatcggct
     2881 acatcgtatt gctgttcatg ttctgtcatc tgtttggtgt gctgggagca gcgtttgctt
     2941 acttcggtgg gcaatgtctg gacgtggcgc tctcgttaat tccaacctta aaggcatttt
     3001 ttcagcgcca ttccttgctt tataacgcag ccggagagaa atcctgatga tgagaaaata
     3061 tttcccgctt gaggcgagcg agcgtctatt tgttgcgatt gaagaagatg atgtcgttga
     3121 tgcacaggtt agtttgccgc cgactattgc actaagttgt accaccgaaa tcattcatga
     3181 taattacgcg ttatgcctgc aattctggct caatggcgtt gatcgtcagg aattactgcg
     3241 tttagttcgc aaacaagcca aaggcgatga gctgactgct gatgagcgca aacaatttaa
     3301 atacatgcga gcgcgctaca agcacctgcg ttttgcgcaa cggctgtatt taaagaagca
     3361 tcaggccgga tttttattcg gcaaaacgac cgtttttctg gggcgttttc aggacggctt
     3421 tcgtaacggt aagaaaaata ttgtgtcgta ttacggcaac ctgttacgga tttatttaag
     3481 ctctccggtc tggtcgttgg tgaactattc attacgccat agccagttag aaagcgtaag
     3541 cagttttatt gcctatcggc aaaagcaaat gcataccctg aaagagatta ttgccaaacc
     3601 gcgactaacc ggcagggaat ttcatgatgt gcgaaaaatc atcagccaac aggtttctta
     3661 ttacgacacc ttaagatcgc ttgatcctga aaataaagag gcgttacaga tttcccgttt
     3721 cctcgccgcc attaatggcc tgatgggaga taaacacgac gacatggtgg cggatgatat
     3781 ggaaaaccgc cagtcgtatg atgcaccggt ggcgctggat agcgatattc gccagcgctt
     3841 agagctgctg atttcgcgtt ttccgctata accggagcaa tgatgtcatc tcgtttgatt
     3901 attgcgctga ttatcatgct tttagccccg ggtgtgcagg cacacaattt cgtcactggc
     3961 aaaacggtta cccctgttta tatccaggaa ggcggcgagc tgcttctgaa tagcgacgac
     4021 gaaattcact accaaaagtg gaacagcaca caattagcgg gaaaagtccg cattattcag
     4081 tatatcgccg ggcgtaagtc ggcgaagaaa aagaactcac tgttaattaa agcggttgaa
     4141 gcggcaaact ttccgcagga tcgttttcag cccaccacca ttgtgaacac cgatgatgcc
     4201 atctttggta ctggctattt tgttgttggc aaaatcgaaa aaaataaacg ccgctatccg
     4261 tgggcgcaat tcattattga cggcaatgga cagggacgcg ttgcctggcg cttgccggag
     4321 cagagttcaa ccattctggt gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc
     4381 agcctgacgc cggaagaggt agatcatgtc attgcgctgg cgcagaaact gatcaatgaa
     4441 taaaaaattc aaaggattga gggcgttagt gagatgaagc tggttgaaca ctacatcatg
     4501 cgtggtacgc gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc
     4561 tactccgcac agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg
     4621 ctggatatcg ttttctacaa agtgctgatt gcactggaga tgttgttacc tgttggtctg
     4681 tatgtgtcag ttggcgtgac gctagggcag atgtataccg actcggaaat taccgctatc
     4741 tctgcggcgg ggggcagtcc gggacgcttg tacaaagccg ttctttatct ggcgataccg
     4801 ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta tacgcagatt
     4861 tatcaactgg agcagcagtc gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa
     4921 tttaacacta acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat
     4981 cgcctgactg acgcgctgat ttatacttct actgccaatc gaacccgcat tttccgcgcc
     5041 cgttcggttg atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcacaacggc
     5101 actgcctatc ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg
     5161 caattacatc tgaatccgct ggatcaaagc cctaacgtca aacgcaaagc aaaatcggtc
     5221 acggagctgg cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc
     5281 cgtggcctga cagcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg
     5341 cggcaagggc gattttcaac attattgcca ctgacgttgc tgtttattgc cattttttat
     5401 ggcggcgacg tctgccgtac gctggtggct aacggcgcga ttcccctcat tcctggtttg
     5461 tggttagtac ccggactcat gctaatgggc ctgctgatgc tggtcgcacg cgacttctct
     5521 ttgctgcaga aattttcccg atgaatgttt tcagtcgcta tttaatccgt catctctttc
     5581 tcggttttgc cgccgccgca gggctattgc tgccgctttt taccaccttc aacctgatta
     5641 acgaactgga tgatgtcaac ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc
     5701 taatgacctt accgcgcaca ctggtcgaac tttcgccgtt tatcgcgtta ttgggtggta
     5761 ttgttggact ggggcagtta tcgaaaaaca gtgagcttac cgccattcgc agcacggggt
     5821 tttctatctt ccgtattgca ctggtggcgc tggttgccgg gatattgtgg actgtttcgt
     5881 taggcgcgat agatgagtgg gtggcgtcgc cattgcagca acaggcgttg caaatcaaat
     5941 cgaccgccac cgcgttgggg gaggacgatg atattaccgg caatatgctg tgggccaggc
     6001 gcggtaatga atttgtgacg gtgaaatcgc tgaatgaaca gggccagcct gtgggcgtgg
     6061 agatatttca ttatcgcgac gatctctcgc tcgaatccta catttatgca cgcagtgcca
     6121 ccattgaaga cgacaaaacg tggattctgc atggtgtgaa tcataaaaaa tggctgaatg
     6181 gcaaagaaac gctggaaata ttagataatc ttgcctggca atcgacattc accagtatga
     6241 atcttgaaga actgtcgatg ccgggcaata gcttttccgt tcgtcagctc aaccattaca
     6301 tcaattactt gcaggaaacc ggacaaccga gcagtgagta tcgcctggca ttgtgggaaa
     6361 agctggggca tcccatttta accctggcga tgattttgct ggcagtgcct tttaccttta
     6421 gcgccccgcg ctcgccgggg atgggcagcc gtcttgctgt aggcgtcatc gttggcttac
     6481 ttacctggat aagctaccaa ataatggtca atctgggatt attatttgcg ctgagtgcgc
     6541 ccgttgctgc actcggttta cctatggcat ttgtgctggc ggcgttgatt ctggtgtatc
     6601 tatttgatag gcaacattaa acccgccgtg gcacaacacc acggcgggct cacattactg
     6661 cttcagagta gcaaatgcct gaatgatggc atcgatttgc tcacgactgt gggcagcatt
     6721 tacgctacag cgcagcagcg tgatgcctgc aggcgcggca ggcggcagga taagattcac
     6781 atacacgccc agcgagataa gctcgcgcca gatgcgtaaa ccttcctctt tcgaaccgat
     6841 tattactggc actaccgggc taatatgtga acttaactca taacccagtt tttgcagacc
     6901 gtcatacaga tggttggcgt tatccatcag cttttgccgc agctctggtt gtgtggcaat
     6961 agttcttaac gacgagcgca cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat
     7021 atacgggcgg ctgccgtagc gcagcacttc catcgcttcc gaaccgacag caaaaccgcc
     7081 aatcgaggcc agacttttac tgaaagtacc aacgataata tccacatcat cttcaacgcc
     7141 gacagcttca gccagtccgc gccccgttgc gcctaacacg ccaaacgaat gcgcttcatc
     7201 gacaatcaaa taaccaccga gacgacgttt gatatcaaca atttccgcca gcggcgcaac
     7261 gtcaccgagc atgctgtaga tgccctcaac gatgataatc gcctctttcg cccgttcacc
     7321 gaggcgaacc atgcgccgtt ccagatcttt agcatcgtta tggcggaaac gaataatttc
     7381 cgcgccaccg agagaacagg cgtcataaat actggcgtgg ctgtcggcat ccagtaacac
     7441 cacggcgtta tggtcggcaa gtgcgctgat gacgcctaaa ttagcggtat aaccggttga
     7501 aaaaacgata gcggtagggc gattgaaaaa ctcggcaatc tctttctcta acgccaggtg
     7561 cggcgcataa ctgccgtttg ccatacgaga accggtggtg ccggtacctt gggccgctaa
     7621 cgcggcctga ccttcgctga tggcatcatg attaaaggtt aaaccgagat aattattggt
     7681 cccggccaga atcacttttt tattgccgat ccggccttcg gtggcggaat acacttcatc
     7741 aatacaggtg ccaaacggtg ttaacccgtt gtcactaaat tgcagtcgct cgccagcaag
     7801 gcgcgcatat ttatcgtata gccccattat ttattctcca gccatggaag tagagtttcc
     7861 attaattgcg caggggtttt gacatccagc agaatattaa taggaatgga aatatcgaat
     7921 ctgtcttcca gcatcattaa gagatccatc actttgatcg actccaggcc aagatcgttg
     7981 acgagatcgc tgtcgggctt aatttccaca ccgttttcga ccaaatcctg taaacaggaa
     8041 aggatgtaat ccattactat ttcacgattt accatagaaa cgttaacaca cctttaaaca
     8101 agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt
     8161 aatatcttca gaaatacggt tattacttgc cgaccagtcg gcgtgggtta attcacgaat
     8221 tttcgagcgg gtcagcatag gttctttacc tgccaggcga ctcaacgcgg tactgatatc
     8281 cgcaaggcag gtgagtaccg gcagaggaat accaaccatt cgcacggaac cgcaacggac
     8341 gttggcggca agttgctgta tgcgttgcca gtcatagccg ccagcgacgc catcgcataa
     8401 ttcataggtt tgcgtctgta cagtttcggc gcttaaccac tgacccactg cttgcgcaaa
     8461 atcggtgacg tgcaggaaag agagctgtgt atctggtgca ccaagtcgtg gcagcaggcc
     8521 gcgcagcatc cagtcaaaca gcggttttaa ctctttatcg ccgggaccat acacggctgt
     8581 cgggcgaaaa acgcccagcg taatttcgtc agccattgca gtcagccgtt gttcggcgat
     8641 gtgtttggaa tttgcgtacc aggagagctc gggatggcgc gccgccagcg aagagataaa
     8701 cagaaaacgt tggcaaaagc agctctcttt tgctgcttgc atcaggcgca ggctgccgtc
     8761 aacgttacag cgggtgaaaa tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac
     8821 cactgcgctg gctccggcaa ccagcttgct aagtgaatga gtatcttcca gcgaaccgcg
     8881 cacccacgtg agattatcat tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg
     8941 aaagccgcgg gcgagcaggt tatcgataat atatttaccg ataaacccgg tagcgcccgt
     9001 caccgcgaca gtttgattca tgccagggat tcctgcacat gaaggcttgc agcataagcc
     9061 ttctgataac gttttttcgc ttccgcacgg gcaggcttgc cggaggatgt tcggggaata
     9121 ctgtggggcg gcaacagatc gatagccgct gttacgccaa attcgctttg gatccgtgct
     9181 gccagcgcgt ggataagctg cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc
     9241 aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt
     9301 tcttgttccg caatatattc aatatcctgc ggccagatat tacggccacg aataataatc
     9361 agatctttaa tacgtccggt gacatacaga taaccgtcca gcagataacc gaggtcgccg
     9421 gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg
     9481 ctcatcaggc tggggccgga gatgcaaata tggcctacca cacgttccgc gaccggcata
     9541 cctgcttcat tgcggatttc aatgccatgt tccggcaacg ctttgccgca gttgacgaaa
     9601 gtcgatacgg cgcgtgtttc agcacccggc gcgacggctt tgccctgata ttctaggatg
     9661 tcgcgatcca cttcgttaac cacaacccca gagccttcat cagagaagct gacagccagc
     9721 gcattttctg ccagtccgta gcacggcatg aacgttttat cgtcaaagtt aacctgacgg
     9781 aaacattcag cgaattgatg gagttgttct gcggagatcg gctcagcacc aataccagcg
     9841 acgcgccagc aggaaagatc cagttcagcg agatcttttt cattcacgcg gcgttggcac
     9901 aattcatagc caaacggcgg cgcaacggaa acggtgccgc gatttttact gatcaattta
     9961 agccattgca aaggacgcat ggcaaaatcc tgagtgcgca aataatctac tgaaagttgc
    10021 gtggcgacgg gggtcaggag aaagccgacc agtcccatat catggtagaa aggcagccag
    10081 gagacgcagc ggtcgccagg gcgtaattta atgccatcgt ggcttatagc acgtagatta
    10141 gccatcactt cgcgatgggt gataatgacg ccacggggaa aacgggtgct gccggaggtg
    10201 tactggaggt aggcgatatc gttcggaact ggacgctgga gcgcaacatc ggcttccggt
    10261 aatgccttaa accaggcgtg gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca
    10321 ttgaccagtg gcaaccactc atcaccagtg ataatagctg cgggctggca acttgccagt
    10381 aaaccctgga gtttggcgct ccaggaatcc cgctgaccga cgcccattgg aatcgccaac
    10441 gggacggcga ctaagccagc atactgacag gcaaaaaaag cctctacaaa cccgctactc
    10501 gtttcggcaa tcagcgccac gcgttcgcct tttttcaggt tcagcgataa caaccgcttc
    10561 gcaccagctt cggcacgcgt ttttaacgtc tgatattcca gttgatcttc aagttggcaa
    10621 cgtctgtcat aaaaattcat tccggcgcta ctcagagcgg cgtagtccaa agcatcaacc
    10681 agcgttggaa aatcggcata gcgcataggt agggaatgcg taaa
//
LOCUS       DAZFFI010000147        10521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFFI010000147 DAZFFI010000000
VERSION     DAZFFI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.147"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..753
                     /gene="ssuE"
                     /locus_tag="ACHJ12_004379"
     CDS             178..753
                     /gene="ssuE"
                     /locus_tag="ACHJ12_004379"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4992075.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            746..1705
                     /gene="ssuA"
                     /locus_tag="ACHJ12_004380"
     CDS             746..1705
                     /gene="ssuA"
                     /locus_tag="ACHJ12_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4992076.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1702..2847
                     /gene="ssuD"
                     /locus_tag="ACHJ12_004381"
     CDS             1702..2847
                     /gene="ssuD"
                     /locus_tag="ACHJ12_004381"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4992077.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2858..3649
                     /gene="ssuC"
                     /locus_tag="ACHJ12_004382"
     CDS             2858..3649
                     /gene="ssuC"
                     /locus_tag="ACHJ12_004382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO4992078.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3646..4413
                     /gene="ssuB"
                     /locus_tag="ACHJ12_004383"
     CDS             3646..4413
                     /gene="ssuB"
                     /locus_tag="ACHJ12_004383"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4992079.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRLRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4620..7232)
                     /gene="pepN"
                     /locus_tag="ACHJ12_004384"
     CDS             complement(4620..7232)
                     /gene="pepN"
                     /locus_tag="ACHJ12_004384"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4992080.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7498..8700
                     /gene="pncB"
                     /locus_tag="ACHJ12_004385"
     CDS             7498..8700
                     /gene="pncB"
                     /locus_tag="ACHJ12_004385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4992081.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREIILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8869..10269
                     /gene="asnS"
                     /locus_tag="ACHJ12_004386"
     CDS             8869..10269
                     /gene="asnS"
                     /locus_tag="ACHJ12_004386"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4992082.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2392 a         2709 c         2812 g         2608 t
ORIGIN      
        1 aacatctaat gactatccaa caaataaata tgcgtgatat ataaagccct ttctttagtt
       61 tattttcaga aaaagataca caaaatttat atttggcatt ttcttgtctc tccgacagac
      121 tgaccgaatt gttatcaatg ttaacaaaaa aagaacaatt ggttataagg agagagtatg
      181 cgtgtcatca ccctggcggg tagtcctcgc tttccttctc gctccagctc cttgctggaa
      241 tatgcgcggg aaaaactaaa tggcctcgat gtagaggttt atcactggaa tctgcaaaac
      301 ttcgccccgg aagatctgct ttatgctcgt ttcgatagtc cggcactcaa gaccttcacc
      361 gaacagctgc aacaggctga tggactgata gtcgctacac ctgtgtataa agccgcctat
      421 tccggcgcgc tgaaaaccct gctcgacctg ctgccagagc gcgctttgca aggcaaagtg
      481 gtgctaccgc tggcgacggg cggtaccgtg gcccatctgc tggcggtcga ttatgccctt
      541 aaaccagttt taagcgcact gaaagctcag gagatcctgc acggcgtgtt tgccgatgac
      601 tcacaagtaa ttgattacca tcacagaccc cagttcacgc caaatctgca aacccgtctt
      661 gataccgcgc tagaaacttt ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac
      721 cttctgtctc tgcgaggtaa tgcccatgcg taacatcatt aaactggcgc tggtgggatt
      781 gcttagcgtc tctacgattg cggttgctgc agaatcctcg cctgaagcgt tacgtatagg
      841 ctatcagaaa ggcagtattg gtatggtact ggcaaaaagc caccagttac tggaaaaacg
      901 ctatccgcaa tcaaaaatct cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc
      961 gttaaacgtt ggcagtattg atctcggcag taccggggat attccgccaa tctttgccca
     1021 ggctgccggg gctgatttgg tatatgtggg cgtcgagcca ccgaagccca aagccgaagt
     1081 gattctggtg gcagaaaaca gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt
     1141 tgcctttcag aaaggttcca gttcacacaa ccttttactg cgtgcactgc gtcaggccgg
     1201 acttaagttt accgatatcc aacccactta cctgacgccc gctgatgccc gcgccgcgtt
     1261 ccagcaaggt aacgttgacg cctgggctat ctgggatccc tactactctg ctgcattatt
     1321 acagggcggc gtgcgggtgc tgaaagacgg caccgatctc aatcaaactg gatcgtttta
     1381 tctggcagct cgtccctatg cagaaaaaaa cggcgctttt attcagggcg tactcgcaac
     1441 ctttagtgag gccgatgcgt taacccgcag ccagcgcgag caaagcatcg ctttactggc
     1501 aaaaacgatg ggcttaccgg caccggtgat tgcctcttac ttagatcatc gccctcctac
     1561 caccatcaaa ccggttaacg ccgaggttgc cgccttacag cagcaaacgg cagatctgtt
     1621 ttatgaaaat cgtctggtgc cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac
     1681 tcaactggaa ggaaaacaat tatgagtctg aatatgttct ggtttttacc gacccatggt
     1741 gacgggcatt atcttggaac ggaagaaggt tcacgcccgg ttgatcacgg ctatctgcaa
     1801 caaattgcgc aagcggcgga tcgtctgggc tataccggtg tgctgatccc aacggggcga
     1861 tcgtgtgaag atgcgtggct ggtggcggcg tcgatgatcc cggtgacgca gcggctgaag
     1921 tttcttgtcg ccctgcgccc cagcgtaacc tcacctaccg ttgccgctcg ccaggccgcc
     1981 acgcttgacc gtctctcaaa tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca
     2041 caagagctgg caggcgacgg cgtgttcctt gatcatagcg agcgctacga agcctcggcg
     2101 gaatttaccc aggtctggcg gcgcttattg ctgggggaaa ccgtcaattt caacggtaaa
     2161 catattcatg tgcgcggagc aaaactgctc ttcccgccga ttcaacagcc ttatcctccg
     2221 ctttactttg gcggatcgtc agacgtcgcc caggagctgg cggcagaaca agttgatctc
     2281 tacctcacct ggggcgaacc gccagaacta gtaaaagaga agatcgaaca agtgcgggcg
     2341 aaagctgccg cgcatggacg caaaattcgt ttcggtattc gtctgcatgt gattgttcgt
     2401 gaaactaacg acgaagcgtg gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa
     2461 actatcgcca aagcacaggc cgcattcgcc cggacggatt ccgtaggaca acagcgaatg
     2521 gcggcgttac ataacggcaa gcgcgacaat ctggagatca gccccaattt atgggcgggc
     2581 gttggcttag tgcgcggcgg tgccggaacg gcgctggtgg gcgatggtcc tacggtcgct
     2641 gcgcgaatca acgaatacgc cgcgcttggc atcgacagct ttgtgctttc gggctatccg
     2701 catctggaag aagcgtatcg ggtcggcgag ttgctgttcc cacatctgga tgtcgccatt
     2761 ccggaaattc cccagccgca gccgctgaat ccgcaaggcg aagcggtggc gaatgatttt
     2821 atcccccgta aagtcgcgca aagctaagga agaaataatg gcaacgccag tgaagaagtg
     2881 gttattgcgc gttgccccct ggtttttacc ggtgggcatc gtggcggtgt ggcaactggc
     2941 ctcctcagtt ggctggctgt cgacgcgtat tttgccctca ccggaagggg tggtgacggc
     3001 gttctggacg ctctccgcca gcggcgaact gtggcagcat ctggcgatca gttcctggcg
     3061 ggcgctgatt ggcttttcaa ttggcggatc gctggggttg attttgggac tgattagcgg
     3121 gctatctcgc tggggagaac ggctgctgga tacctcaatt cagatgttgc gcaacgtacc
     3181 gcatctggcg ctgatcccgc tggtgatttt gtggtttggc attgatgaat ccgcaaaaat
     3241 ctttctggtg gcgctcggca cgctattccc catttatatc aacacctggc atggcatccg
     3301 taatatcgat cgcggtctgg tggaaatggc gcgtagctat ggattatccg gcataccgct
     3361 gtttatccat gtgatcctgc ctggtgccct gccctcaatt atggtcggcg tgcgttttgc
     3421 gttgggcctg atgtggctga cgctgattgt tgccgaaacc atttctgcca attcaggcat
     3481 tggttatctg gcgatgaatg cgcgggagtt tttgcaaacg gacgtggtgg tagtcgctat
     3541 tattctttac gccctgcttg gcaaacttgc tgacgtcagc gcgcagctgt tagagcgcct
     3601 ctggttgcgc tggaacccgg cttatcattt gaaggaggcc acggtatgaa tactgctcgt
     3661 ctgaaccagg gcacaccatt gttgctcaat gcagtaagca aacattacgc ggaaaatatc
     3721 gtcctgaacc aactggattt acatattccg gcaggtcagt ttgtggcggt ggtgggccgc
     3781 agcggtggtg gcaaaagtac cctgctgcgc ctgctggcag gtctggaaac gccaaccgca
     3841 ggcgatgtgt tagcgggcac cacaccgctg gctgagattc aggaagacac gcgaatgatg
     3901 tttcaggatg cgcgtctgct gccgtggaaa tcggtgattg ataacgttgg gttaggcctt
     3961 aaaggccagt ggcgcgatgc cgcgcgtcgg gcgttggctg cagtaggact ggagaatcgc
     4021 gccggggaat ggcctgccgc actttctggc gggcagaaac agcgtgtggc gctggcaaga
     4081 gcgttgattc atcgaccggg tttattgttg cttgatgaac cgctcggggc gctggacgcc
     4141 ttaacgcgac tcgagatgca ggatttgatt gtgtcacttt ggcagcagca cggctttacc
     4201 gtactgttgg tgacgcatga tgtgagcgaa gcggtagcaa tggctgaccg ggtgttgtta
     4261 attgaagagg gaaaaattgg tctggatttg acagtggata tcccccgtct gcgtcgatta
     4321 gggtcggtgc gattagcgga gctggaagcg gaagtattgc aacgggtaat gcggcgtggg
     4381 cattctgaac aactgattcg gcgtcatggt taatatgccg gatgtggcgc acaaaaccag
     4441 cattttcaac ggactagtag gcctgataag acacgtcaag catcgcatca ggcagttggg
     4501 cacggctgcc ggatgcggcg tgaacgcctt atccagccta caaaaccagc aatttcaacg
     4561 gagttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcattttggc tcaaaagtat
     4621 caagccagtg ctttggtgat cttctcgtac agatcgccag agagattttc cagccctttc
     4681 aactgttcca gcgccgcgcg cattttctcc tgacgtttgg catcataacg tttcaggcga
     4741 atcagcggtt caatcagacg tgaagctacc tgcgggttac ggctgttgag gtcggtaagc
     4801 atttccacca ggaactggta accgctgcca tcttcggcat ggaacgctgc cgggttgctg
     4861 cccgcaaacg caccaatcaa cgaacgaata cggttcgggt tgctcatggt aaatgagcga
     4921 tgctgcaaca gaccacgcac cgtctccagc acattcgccg ccgggctggt ggcttgcagg
     4981 ataaaccatt tatccatcac cagaccgtcc tgatgccact tatcgtcgta ctcctgcatc
     5041 agcgcgtcac ggcaaggcag ctgtgcagca accgccgcag aaagcgctgc cagcgcatcg
     5101 gtcatattgt ttgcttcatg gtattgcttg ctcaccagca cgtcagccag atgcgtttca
     5161 ccaaaagcaa ggaagcgcag gcaggcatta cgcagagtgc gttttgcaat atcttcatgc
     5221 tcaacacggt actcgctctg gtaattcgcg ttgtaaatag ccagcagctc atccgccagt
     5281 tcagtcgcca gagtacgagt gagtgcttcg cgtacttcgg caatagcaat cgggtcgatg
     5341 atatcgaaca gttcagccat ttcattgaca gaaggcagcg tcaggatttc tgccgccagc
     5401 gccggatcaa tcttctcatc gagcagcacc gcgcggaaag cgtcagccac atgtaccggc
     5461 agagacagcg gctgcccttg ctgatgacgg gcgacgttca gcttgatgta ggttgccagc
     5521 aaactttgcg ccgcatccca acgggagaaa tcattacgcg catgacgcat caggaaggtc
     5581 agttgctgat cgctccactt atattccagt ttcactggcg cagagaattc gcacagcagc
     5641 gcaggcaccg gctggaagta gacattatca aaaacaaagg tctgttccgc ctgggtgacg
     5701 ttcagcacgg aattcaccgg atgaccgcct ttctgcaacg ggatcacttt gccttcgttg
     5761 tcatacaatt cgatggcaaa tggaatatgc agcggctgtt tttctgcctg atccggcgtg
     5821 gctggcgtgc gctggctgat ggtcagggtg tactgctcgg tttccggatt gtagtcgtct
     5881 ttgacggtca caatcggcgt gccggactgg ctgtaccaac ggcggaaatg ggagagatcg
     5941 acattcgacg catcttccat tgcctgcaca aagtcgtcgc aggttgccgc gctgccatca
     6001 tggcgctcga aatatagttg catccctttc tggaagtttt cttcgcccag cagggtgtgg
     6061 atcatgcgaa tcacttccgc gcccttctcg taaacggtca gggtgtagaa gttgttcatc
     6121 tcaatgacca tatccgggcg gatcgggtgc gccatcgggc tggcgtcttc cgcaaactgc
     6181 aatccgcgca tggtgcgcac attattgata cggtttaccg cgcgggaacc aaggtcagag
     6241 ctgaattcct gatcgcggaa gacggttaaa ccttctttca ggctgagctg gaaccagtcg
     6301 cggcaggtca ctcggttacc ggtccagtta tggaaatatt catggccgat aacgcgttca
     6361 atatcgagat aatctttgtc ggtggcggtg tcggtgcggg ccaacacata tttggagtta
     6421 aagatattca gacccttatt ctccattgcg cccatattga agaaatccac cgcgacgatc
     6481 atatagatgt cgaggtcata ctccaggcca aagcgttctt catcccattt catggagttt
     6541 ttcagcgagg tcatcgccca cggcgcgcga tcaaggttgc cgcgatcgac gtacagctcc
     6601 agtgctactt cgcgaccaga acgcgtggta aaggtatcgc gcagtacatc aaagtcgcct
     6661 gccaccagcg caaacaggta gcacggtttc gggaacgggt cctgccactg tacccaatgg
     6721 cgtccgtttt ccagttcgcc ttgcgcaacg cggttaccat tggaaagcag gaagggatat
     6781 ttgattttat cggcaataat tttggtggta aaacgcgcca gcacgtccgg gcggtcgaga
     6841 taatacgtaa tatggcggaa accttcggct tcacactggg tgcaaagcgc atcgcctgac
     6901 tgataaagcc cttccagcgc cgtattcgcc gccgggctta tttcattaat gatcttaagc
     6961 gtaaaacgct ccggcaaatt actgatgacc agtgcgccct cttcttcttt ccaggcggtc
     7021 cacggctcat cattaatatg aacagaaacc agtttgaggt cttcgccgtt gagacgaagc
     7081 ggagcatctg atgcaccatg acggacagcc tggctgaccg cggtaacgac cgtcttttgc
     7141 gcgtcgaggt caaaggtcaa gtcaatatca gtaatctggt aatccggcgc acgataatcg
     7201 tgacggtatt tggcttgtgg ctgttgagtc ataaataacc tttagcatct tttatagagt
     7261 ctggtgttca gtctattcct gttgcgtaaa tcgcgctatg cagaatcttc atcttttcag
     7321 gtacaaacgc ctttattgct acatttttat aacatacacc gcgtaatgcc atcgaccaga
     7381 aaggtggcat atggtgtgat cggggttcaa taaattgcga aacaaggtat actccagcag
     7441 ttcctgaaga tgtttattgt actaaacgct cctgtacgag gacgctactg cgcacctatg
     7501 acacaattcg cttctcctgt tctgcactcg ttgctggata cagatgctta taagttgcat
     7561 atgcagcaag ccgtgtttca tcactattac gatgtgcatg tcgcggcgga gtttcgttgc
     7621 cgaggtgacg atctgctggg tatttatgcc gatgctattc gtgaacagat tcaggcgatg
     7681 cagcacctgc gcctgcagga tgatgaatat cagtggctct ctgctctgcc tttctttaag
     7741 gccgactatc ttaactggtt acgcgagttc cgctttaacc cggaacaagt caccgtatcc
     7801 aacgataatg gcaagctgga tattcgttta agcggcccgt ggcgtgaaat catcctctgg
     7861 gaagttcctt tgctggcggt tatcagtgaa atggtacatc gctatcgctc accgcaggcc
     7921 gacgttgcgc aagccctcga cacgctggaa agcaaattag tcgacttctc ggcgttaacc
     7981 gccggccttg atatgtcgcg cttccatctt atggattttg gcacccgtcg ccgtttttct
     8041 cgcgaagtac aagaaaccat cgttaagcgt ctgcaacagg aatcctggtt cgtgggcacc
     8101 agcaactacg atctggcgcg tcggctttcc ctcacgccga tgggaacaca ggcacacgaa
     8161 tggttccagg cacatcagca aatcagcccg gatctagcca acagccagcg agctgcactt
     8221 gctgcctggc tggaagaata tcccgaccaa cttggcattg cattaaccga ctgcatcact
     8281 atggatgctt tcctgcgtga tttcggtgtc gagttcgcca gtcgctatca gggcctgcgt
     8341 catgactctg gcgacccggt tgaatggggt gaaaaagcca ttgtacatta tgaaaagctg
     8401 ggaattgatc cacagagtaa aacgctggtt ttctctgaca atctggattt acgcaaagcg
     8461 gttgagctat accgccactt ctcttcccgc gtgcaattaa gttttggtat tgggactcgc
     8521 ctgacctgcg atatccccca ggtaaaaccc ctgaatattg tgattaagtt ggttgagtgt
     8581 aacggtaaac cggtggcgaa actttctgac agccctggca aaactatctg ccacgataaa
     8641 gcgtttgttc gggcgctgcg caaagcgttc gaccttccgc atattaaaaa agccagttaa
     8701 tatcatcagg gagctaatcg gctccctttt tttaccttta attccgaaat ctttcgctgc
     8761 atttgcgaat tctgcttgtc tgattgcaga tgccaggtaa cataggtatc cccccattta
     8821 cgggtggaca agtgtttatt ttttccgact attaacagag agaatattat gagcgttgtg
     8881 cctgtagccg acgtactcca gggccgcgta gccgttgaca gcgaagtcac cgtacgcgga
     8941 tgggtacgta cccgccgaga ttcaaaagct ggcatctcct tcctcgccgt ttatgacggt
     9001 tcctgctttg atcctgtaca ggctgtcatc aataattctc tgcccaatta caatgaagac
     9061 gtcctgcgtc tgaccaccgg ctgctcggtc attgtgacgg gtaaagtcgt ggcgtcgccg
     9121 ggccaggggc aacaatttga aattcaggcc agcaaagttg aagttgctgg ttgggttgaa
     9181 gatccagaca cttacccgat ggcggcaaaa cgccacagca ttgagtatct gcgtgaagtc
     9241 gctcacctgc gtccgcgcac aaacctgatt ggtgccgtcg cgcgcgttcg ccatacgctg
     9301 gcgcaggcgc tgcatcgctt ctttaacgag cagggattct tctgggtttc aacgccactg
     9361 attaccgcat ctgataccga aggtgcaggc gaaatgttcc gcgtttctac gctggatctg
     9421 gaaaacctgc cgcgtaacga tcagggcaaa gtggatttcg acaaagactt ctttggtaaa
     9481 gagtctttcc tgaccgtatc tggccagttg aacggcgaaa cctacgcttg cgcattatcc
     9541 aaaatttata ccttcggccc gactttccgt gctgaaaact ccaacaccag ccgtcacctg
     9601 gcggaattct ggatgctgga gccggaagtg gcgtttgcta acctgaacga tatcgcgggt
     9661 ctggctgaag ccatgctgaa atatgtcttc aaagcggttc tcgaagaacg cgcagacgac
     9721 atgaaattct tcgctgaacg cgtagataaa gatgccgttt cacgtctgga acgcttcatt
     9781 gaggccgatt tcgcgcaggt ggattacacc gacgcagtga ccattctcga aaactgcggc
     9841 aggaagtttg aaaacccggt ttactggggc gtcgatctct cttctgagca tgagcgttat
     9901 ctggcggaag aacactttaa agcaccggta gtggttaaaa actatccgaa agatattaaa
     9961 gcgttctata tgcgcctcaa cgaagacggt aaaaccgttg cggctatgga cgttctggct
    10021 ccgggcatcg gtgagatcat tggtggctcc cagcgtgaag aacgtctgga cgttctggac
    10081 gagcgtatgc tggaaatggg cctgaataaa gaagattact ggtggtatcg cgatctgcgt
    10141 cgctacggta ctgttccgca ttcaggtttc ggtcttggtt ttgaacgcct gattgcttac
    10201 gtaactggcg tgcaaaacgt gcgtgatgtg attccgttcc cacgtactcc gcgcaacgcc
    10261 agcttctgat tttcttcctc aaagagccag cgcctgctgg ctctttttta tcctttctgt
    10321 caagttatct gtttgttaag tcaagcaatc tatttgcaac cccgccataa cgatcatcct
    10381 gttacggaat attacattgc aacatttacg cacaaaaaat aacccgcatt cttattgcgg
    10441 attagttttt tcttagctga tagcacaatt ttcatactat tttttggcat tctggatgtc
    10501 tgaaagagga ttttgtgcca g
//
LOCUS       DAZFFI010000148        10437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFFI010000148 DAZFFI010000000
VERSION     DAZFFI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.148"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(142..2175)
                     /gene="betT"
                     /locus_tag="ACHJ12_004387"
     CDS             complement(142..2175)
                     /gene="betT"
                     /locus_tag="ACHJ12_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO4992083.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMMLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2304..2891
                     /gene="betI"
                     /locus_tag="ACHJ12_004388"
     CDS             2304..2891
                     /gene="betI"
                     /locus_tag="ACHJ12_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4992084.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2905..4377
                     /gene="betB"
                     /locus_tag="ACHJ12_004389"
     CDS             2905..4377
                     /gene="betB"
                     /locus_tag="ACHJ12_004389"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4992085.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4391..6061
                     /gene="betA"
                     /locus_tag="ACHJ12_004390"
     CDS             4391..6061
                     /gene="betA"
                     /locus_tag="ACHJ12_004390"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4992086.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6274..6939
                     /gene="ykgH"
                     /locus_tag="ACHJ12_004391"
     CDS             6274..6939
                     /gene="ykgH"
                     /locus_tag="ACHJ12_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO4992087.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICIAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(7185..7880)
                     /locus_tag="ACHJ12_004392"
     CDS             complement(7185..7880)
                     /locus_tag="ACHJ12_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO4992088.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7873..9300)
                     /locus_tag="ACHJ12_004393"
     CDS             complement(7873..9300)
                     /locus_tag="ACHJ12_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO4992089.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHLAQEKKNG"
     gene            complement(9311..10030)
                     /locus_tag="ACHJ12_004394"
     CDS             complement(9311..10030)
                     /locus_tag="ACHJ12_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO4992090.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2646 a         2801 c         2582 g         2408 t
ORIGIN      
        1 aaaatataaa atcatgaagt gatacaagag gaattgcagg atatgacatg acaatcatat
       61 ataatatatg cataattttt tacagcgagt caatgattcc cgcgccatat gcagacatgg
      121 cgcggtttta tgcaaaaaca atcacgcgtc cgggaacatc acgctatggc ccggcgcttc
      181 acgatggaga tgaataaagt taaggtgccg ctcgtactgg tcaagaatat cggtgatcac
      241 ctgctctttg ctgtagtcca tcaggtcgtt gccctggctg ccttctaaca ggaaggtttc
      301 cagccggtag taggtcgatt taccgctacg tgcgcggtag gtaaagcccg gcaccgaata
      361 ttgctgcggc caaatctgat agacaaagtt ttgttcttcg cccatatgca ccaacaaatc
      421 cagatgcccc aactgttgtc cctcttccgg cggcaggctt tttagctcca cgtacgcgcc
      481 gcgcaaccgc agctcctgcg ccacttcttc cattgccggg taacagaccg tctccatcat
      541 ctgtttagtg taacgcgtgc ccggataatt catcaggcgc gagagacgtt ttttccagct
      601 caggcgatcc tgaagcccca gcggtcgcgg cgcggtatcg cggttggcac tttcacggcg
      661 gtaatcttct accttcagag atttatacaa ccccgccatc acgaagaaaa tcacaaagct
      721 gaacggcagc cccatgatta ccgtggtgtt ttgcagcgcg gatatcccgt tggtcatcag
      781 catgccgagc gtcagcagac caatcgccac cgaccagaag acgcgcagcc agccgggggc
      841 gtcgctgttg atatctttaa gctgcgaggt gaaattcccc agcaccagcg caccggagtc
      901 cgccgaggtc acataaaaca gcaggccagt aatggtggcg acggaggcgc taaaggtaaa
      961 cgccggatac tgcgccagca ggctgtagaa gccgcgctcc ggatggacca tcgcttcctc
     1021 ggcaaatgcc gcgccgccgt ggatgatttc atacagcgcg ctattgccga acaccgagag
     1081 ccataacagc gtgaaggtaa acggaataat caacgtgccc agcacgaact ggcgaatggt
     1141 acgcccacgc gagatacgcg ccaggaacaa gccgacaaac ggcgaccatg ccacccacca
     1201 tgcccagaag aagagcgtcc agttattcat ccactcaact ggacggtcga aggcaaaact
     1261 gttgagcatc atgcccataa agcgattcac atagtcgcca acattcagca ccagcgcatt
     1321 aagcaggaac gaggtgtcgc ccataaacaa tacgaacagg atcaatccca gcgccagcgc
     1381 aacgttaagt tccgataaca cacgaatccc cttatcgaca ccggaggtga cagagatcgt
     1441 ggcgattatc accgacaagg cgatcagtgc cgcttttgcc gccatcgaat cgggaatatc
     1501 aaacagtacg ctcaagccat agttaagctg caccacaccg ataccgagcg tagtggcaat
     1561 accgaagatg gtgccgatca ccgctgcaat atccactgag tgccctatcg gcccgttaat
     1621 ccgtttaccg aagatcgggt acagcgccga gcggatggtg agcggcaaat tataacgata
     1681 gctaaagtat ccgagcgcca tgcccatcag cgcatacatc gaccagccgg ttaagccgta
     1741 gtgaaacagc gtccagacca tcgcctgacg cgcggcctca atcgtctgtc ccgcgccttc
     1801 cggcggctgc atatactgcg ttaccggttc ggctacggag aagaacatca ggtcgatacc
     1861 gatcccggca gcaaacagca tcgccgccca actcagcagg ctgaattccg gtttggattg
     1921 ttctggcccg agcttcaccg aaccaaaacg cgaacaagcg atacagacca caaagacaat
     1981 atagagcgtt gccgccagta gatagtacca accgaaggtt ttagaaaccc agtccagcgt
     2041 gcggccaatc cacagggccg agaagtcgcg aaacaggatc gttgtcaggg aaaacaacaa
     2101 aatcagtccg gcggaggtgt aaaacaccac cggattgatt ttgtcctttt ccctgctgtg
     2161 tgaaaggtct gtcatccagt atccccactg ttattgttac catttaaaat caaattcgta
     2221 acaattaaga cacattttat attgaacgtc caatcaataa ccgctttaat agataaacaa
     2281 cgctgatgaa tggagtggcg aaaatgccca aattggggat gcagtcgatc cggcgcagac
     2341 aactgatcga cgccacactg gaagcaataa atgaagtggg catgcacgat gcaacgatcg
     2401 cgcagatcgc ccgccgtgca ggcgtttcta cggggatcat cagccactat ttcagggaca
     2461 aaaatggtct gctggaagca accatgcgcg atatcaccag tcagctgcgt gacgcggttt
     2521 tgaatcgatt acatgcactt ccgcagggca gtgcagagca gcgattacag gcgattgttg
     2581 gcggaaactt cgatgaaacg caggtgagca gtgcggcgat gaaagcctgg ctggcgttct
     2641 gggccagcag tatgcatcag ccgatgctct atcgtttaca gcaggtcagt agccgccgct
     2701 tattgtcgaa tctggtgagc gaatttcgcc gcgaattgcc gcgccaacag gcacaggaag
     2761 cgggctacgg ccttgccgcg ctgattgacg gattatggct gcgcgcggca ctgagcggca
     2821 aaccgctgga taaacctctc gcacactcgc tgacccgtca ctttattact cagcatttac
     2881 ccaccgatta accgaggaga cgtgatgtcc cgaatggcag aacagcagct ttatatacat
     2941 ggtggttata cctccgccac cagcggtcgc accttcgaga ccattaaccc ggccaacggt
     3001 aacgtgctgg cgaccgtgca ggccgccggg cgtgaggatg tcgatcgcgc cgtgaagagt
     3061 gcccaacagg ggcaaaaaat ctgggcggcg atgaccgcta tggagcgctc gcgtattctg
     3121 cgtcgggccg ttgatattct gcgtgaacgc aatgacgaac tcgcaaaact ggaaaccctc
     3181 gacaccggaa aagcatattc ggaaacctca accgtcgata tcgttaccgg tgcggacgtg
     3241 ctggagtact acgccgggct gatcccggcg ctggaaggca gccagatccc gttgcgtgaa
     3301 acgtcctttg tgtatacccg ccgcgaaccg ctgggcgtag tggcagggat tggcgcatgg
     3361 aactacccga tccagattgc cctgtggaaa tccgccccgg cgctggcggc aggcaacgca
     3421 atgattttca aaccgagcga agttaccccg cttaccgcgt taaagctggc tgaaatttac
     3481 agcgaagcgg gcctgccgga cggcgtattt aacgtgttgc cgggcgtggg cgcggagacc
     3541 gggcaatatc tgaccgagca tccgggcatt gccaaagtgt catttaccgg cggtgtcgcc
     3601 agcggcaaaa aagtgatggc taactcggcg gcctcttccc tgaaagaagt gaccatggaa
     3661 ctgggcggta aatcaccgct gatcgttttc gatgatgcgg atctcgatct cgccgccgat
     3721 atcgccatga tggcaaactt cttcagctcc ggtcaggtgt gtaccaatgg cacccgcgtc
     3781 ttcgttccgg cgaaatgcaa agccgcattt gagcagaaaa ttctggcgcg cgttgagcgc
     3841 attcgcgcgg gcgacgtttt cgatccgcaa actaacttcg gcccgctggt cagcttcccg
     3901 catcgcgata acgtgctgcg ctatatcgcc aaaggcaaag aggaaggcgc gcgcgtactg
     3961 tgcggcggcg atgtactgaa aggcgatggc ttcgataacg gcgcatgggt tgcaccgaca
     4021 gtgttcaccg attgcagcga cgatatgacc atcgtgcgtg aagagatctt cgggccagtg
     4081 atgtcgcttc tcacttatga atctgaggac gaagtgattc gccgcgccaa tgataccgac
     4141 tacggcctgg cggcgggcat cgtgacggcg gacctgaacc gcgcgcatcg cgtcattcat
     4201 cagctggaag cgggtatttg ctggatcaac acctggggcg aatccccggc agagatgccc
     4261 gttggcggct acaaacactc cggcattggt cgcgagaacg gcgtgatgac gctccagagt
     4321 tacacccagg tgaagtccat ccaggttgag atggctaaat tccagtccat attctaacca
     4381 ggaggtttat ttgcaatttg actacatcat tattggtgcc ggctcagccg gcaacgttct
     4441 cgctacccgt ctgactgaag atccgaatac ctccgtgctg ctgcttgaag cgggcggccc
     4501 ggactatcgc tttgacttcc gcacccagat gcccgcagcg ctggcgttcc cactacaggg
     4561 caaacgctac aactgggcct atgaaacaga gcctgaaccg tttatgaata accgccgcat
     4621 ggagtgcgga cgcggtaaag gcctgggtgg atcgtcgctg atcaacggca tgtgctacat
     4681 ccgtggcaat gcgctggatc tcgataactg ggcgcaagaa cccggtctgg agaactggag
     4741 ttatctcgac tgcctgccct actaccgcaa ggccgagact cgcgatgtgg gcgagaacga
     4801 ctaccacggt ggcgatggcc cggtgagcgt caccacctcc aaacccggcg tcaatccgct
     4861 gtttgaagcg atgattgaag cgggcgtgca ggcaggctac ccgcgcacgg acgatctcaa
     4921 cggctatcag caggaaggtt ttggcccgat ggatcgcacc gtcacgccgc agggccgtcg
     4981 cgccagtacc gcgcgcggtt atctcgatca ggccaaatcg cgccctaacc tgaccattcg
     5041 tactcacgct atgaccgatc acatcatttt tgacggcaaa cgcgcggtgg gcgtcgaatg
     5101 gctggaaggc gacagcacca tcccaacccg cgcaacggcc aacaaagaag tgctgttatg
     5161 tgcaggcgcg attgcctcac cgcagatcct gcaacgctcc ggcgtcggca acgctgaact
     5221 gctggcggag tttgatattc cgctggtgca tgaattaccc ggcgtcggcg aaaatcttca
     5281 ggatcatctg gagatgtatc tgcaatatga gtgcaaagaa ccggtttccc tctaccctgc
     5341 cctgcagtgg tggaaccaac cgaaaatcgg tgcggagtgg ctgtttggcg gcactggcgt
     5401 tggtgccagc aaccactttg aagcaggtgg atttattcgc agccgtgagg aatttgcgtg
     5461 gccgaatatt cagtaccatt tcctgccagt agcgattaac tataacggct cgaatgcagt
     5521 gaaagagcac ggtttccagt gccacgtcgg ctcaatgcgc tcgccaagcc gtgggcatgt
     5581 gcggattaaa tcccgcgacc cgcaccagca tccggcgatt ctgtttaact acatgtcgca
     5641 cgagcaggac tggcaggagt tccgcgacgc aattcgcatc acccgcgaga tcatgcatca
     5701 accggcgctg gatcagtatc gtggccgcga aatcagcccc ggtgtcgaat gccagacgga
     5761 tgagcagctc gatgagttcg tgcgtaatca cgccgaaacc gccttccatc cgtgcggtac
     5821 ctgcaaaatg ggctacgacg agatgtccgt ggttgacggc gaaggccgcg tgcatgggct
     5881 ggaaggcctg cgcgtggtgg atgcgtcaat tatgccgcag attatcaccg ggaatttgaa
     5941 cgccacgaca attatgattg gcgagaaaat agcggacatg attcgtggac aggaagcgtt
     6001 gccgaggagc acggcgggat attttgtggc aaatgggatg ccggtgagag cgaaaaaatg
     6061 agtcgtgatg tgaactaacg caggaaccga tcagattgag agttaccgtt ccagagaggg
     6121 ggaccgaatc cttatataaa cactgaggta actctcatgc ttcatattca gtattcatga
     6181 tgctagcttt ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacct
     6241 tatctgcggc agattttatt ttctggatat attatgcgtg aacaaatcaa acaggatatc
     6301 gatctgattg agattttatt ttatctgaag aaaaagattc gtgttatcct ttttattatg
     6361 gctatatgta tagctatggt gctgttgttt ctgtatatca ataaagacaa tataaaagtg
     6421 atttacagcc taaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat
     6481 aataattttg cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt
     6541 tttcacacca gcccagatgt caagaacaga gaaataaggc tggaatggtc aggagataag
     6601 agagctttac caactgctga agaggaaata tctcgcgtgc aggcctctat tatcaaatgg
     6661 tatgcgtcag aatatcataa tggcaggcaa gttctcgatg agatacaaac gccttcagca
     6721 attaacagtg agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg
     6781 aacggtgatg gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc
     6841 atttgcctgg ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc
     6901 aaaaaaatgg tagatgaata ccaacaaaac tctgggtagt aataaacagc attctgatag
     6961 agagtgcctg tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg
     7021 gatgcctgtc tttttcactg taaatatcct ggcattacta atgccaggat atttacagaa
     7081 gagcagcata accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac
     7141 ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata
     7201 atcagataca ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc
     7261 gccgttgaac tagggccgct aatgatgtta atgcaggacg gcattcgttc accggcctgc
     7321 gctttctgat gcaatttttc tgcgagttgc gctacacgcg gcaagatagt gcttttacgc
     7381 aggataaaaa gagaagattc cgggagcagg ctcaatgaac gcccgcgccc ggcggcggaa
     7441 aaaagaacca cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta
     7501 gcctgctctg cttgtgatat gttctcaata cctttcgccg gatcccaaac aacggcattg
     7561 cattcctgct gcaaacgttc gctaatcccc aattcctcta gcctcgtgtc accgctaatc
     7621 acgaccgact gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc
     7681 tcgctggtca gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca
     7741 cagcgctgct gttggttaag tgcggtaagc cgctcgttag catagttgtt aagcggcgca
     7801 tcttctgctt gcggttcaag tcgcaacggg cgacccagtg cctgagcaac gttattcaaa
     7861 aattcgctcc gattatccat tctttttctc ctgcgccaga tgtttcttaa accaactacg
     7921 gaaactctct ccgtcagctt caggaagatc gcgtgcttcc atccagtcac taatcgcgcc
     7981 aaatttgagt ggtgttttgc cgccattgat aaaccagctt gccgcatgag caccggccat
     8041 catcccgact ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg
     8101 ttgctctgct tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag
     8161 ttttgacagc ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc
     8221 gtagggtaaa tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat
     8281 tggccctgga taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt
     8341 attcatacaa gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac
     8401 ctcagaacgc ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc
     8461 cgcttcgcgc ggtcctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact
     8521 gcgcgccagc atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat
     8581 tcccatcact gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt
     8641 ggtcaccagg cataccgaac ctgtctctgc caccgcgaaa ttacagccgg taatacctat
     8701 ttcagcactg aggaaatctt cgcggatttt ttgccggatg aataaggtca tcgcttcagg
     8761 cgtttccggc ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc
     8821 tttatgaatt gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata
     8881 ttcacccaga tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc
     8941 aatctcttcg gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc
     9001 aacctgtaaa atgtagcggg tagcgtcttc tttggttttt gcaaaccaga cgtgaccgcc
     9061 gttttgcgtc actttttctg agagctggta cagataagcg tcgagattac tcagaacatg
     9121 atcacgtatc tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat
     9181 tttttgccga tttgccccaa tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg
     9241 atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat
     9301 ctttatatcc tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc
     9361 cttcccgttg taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat
     9421 actcagggcg gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt
     9481 cggccatttt gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg
     9541 tcaacagctc cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca
     9601 gcttacgggc caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta
     9661 catcgactac ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa
     9721 ccttttcggc acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta
     9781 cggcataggt gcaagagcca gctggtgaaa taatgggatc gtcgttatcc tccagtgcgg
     9841 cgatcagatt tttcatccct ggaatcgctt ctttgatata accactattg atcgcaggct
     9901 gaccgcagca tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca
     9961 gcacggagtc tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat
    10021 tgacattcac aactaatact ccattacttc atgcccattt atgccgatgg ttcccctcct
    10081 tccgggggat caatcatcaa taaatatggc gttctttttt aattggctag caacatacca
    10141 cccctcattt gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt
    10201 ttatttcttt cggcatcgct cacattttat tgggaaatcg acgatcattt ttggcataag
    10261 aaatccattt taaaatggct acatccctta tacctattca gagctactta ttcaggggtt
    10321 attgagatat acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa
    10381 tagtcgggat tttatttaat tgaagcaatt tagtttgcat ttttatttaa aaactga
//
LOCUS       DAZFFI010000149        10374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFFI010000149 DAZFFI010000000
VERSION     DAZFFI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.149"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..1914
                     /gene="ydeN"
                     /locus_tag="ACHJ12_004395"
     CDS             232..1914
                     /gene="ydeN"
                     /locus_tag="ACHJ12_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4992091.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1966..3123
                     /gene="ydeM"
                     /locus_tag="ACHJ12_004396"
     CDS             1966..3123
                     /gene="ydeM"
                     /locus_tag="ACHJ12_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4992092.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3089..3211)
                     /locus_tag="ACHJ12_004397"
     CDS             complement(3089..3211)
                     /locus_tag="ACHJ12_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992093.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3414..5099
                     /gene="yddA"
                     /locus_tag="ACHJ12_004398"
     CDS             3414..5099
                     /gene="yddA"
                     /locus_tag="ACHJ12_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4992094.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            5137..7509
                     /gene="yddB"
                     /locus_tag="ACHJ12_004399"
     CDS             5137..7509
                     /gene="yddB"
                     /locus_tag="ACHJ12_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4992095.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYNKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7566..10349
                     /gene="pqqL"
                     /locus_tag="ACHJ12_004400"
     CDS             7566..10349
                     /gene="pqqL"
                     /locus_tag="ACHJ12_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4992096.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3052 a         2291 c         2264 g         2767 t
ORIGIN      
        1 tcggcatttt gtcagtaaaa tgcaataatt tattaaatat caatagatta gttgtttatc
       61 ggcgagaaat tacttagtag aacagaaagt aatgtcaacg ctttatggac tgttatttcc
      121 ctttttttag ctaaatctgc tatctcttta tgtgactaac ttcacttaca cccacttatt
      181 tctcttcgta aaattacttc ggaattaagt acaataagaa gaggaacatt tatgaagtct
      241 gcattaaaga aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct
      301 gcatttgctg ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct
      361 ttctcagact ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtacta
      421 accatggatg atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa
      481 acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa
      541 gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acggtttact
      601 aacggctatg tggcacacgg tgtttccggc ccctcccgtg ccgcaataat gaccggtcga
      661 gctcccgccc gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca
      721 gaaactttct tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa
      781 tggcacttgt caaaaatcag taatgtgccg gtaccggaag ataaacaaac gcgtgactat
      841 catgacaact tcaccacatt ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat
      901 tactttatgg gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa
      961 aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt
     1021 ggcgttgttg atcgtgccaa aacacttgac cagccattta tgctttacct ggcttataat
     1081 gctccgcacc tgccaaatga taatcctgca ccggatcaat atcagaagca atttaatacc
     1141 ggtagtcaaa cagcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa
     1201 cgcattctcg aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc
     1261 tccgataatg gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat
     1321 aagagtcaga cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa
     1381 cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc gacagctctt
     1441 gatgcagccg atatcagcat tccaaaagac cttaagctgg atggcgtttc cttgctgccc
     1501 tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat
     1561 tctcactggt ttgacgagga aaatattcca ttctgggata attaccacaa atttgttcgc
     1621 catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca attctcttat
     1681 acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc
     1741 tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt
     1801 aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag
     1861 gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac
     1921 cttcatgcgg cggaattttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc
     1981 aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag
     2041 tcgcagttta ctcatgaaaa atggatggat gacagtactc tgaaagagtt catcaaacaa
     2101 tatatcgcag cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg
     2161 gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa
     2221 cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt
     2281 ctcaaagaac atgaatttct ggttggtatc tcgatcgatg gcccccagga gttacatgac
     2341 cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag
     2401 cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc
     2461 cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt
     2521 atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca
     2581 ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt
     2641 tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt
     2701 gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc ctgcaaggat
     2761 aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag
     2821 tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca
     2881 gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg catataaacc tatctgcaat
     2941 ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc
     3001 gaaggttata aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggtggagtta
     3061 gctaagaaca gagtaccgct ttaccacatt atggatgttg caaaacaaat ggagaataat
     3121 taataacgct ctcttttaac tccattctta gcctccttct tatgacctta acaaggcacc
     3181 cgacataaat tcctgtcggg tgtttcgtca tcatcatctt cccttgcgaa aatcctggct
     3241 tacgtggtat gcgtcagggc attagcaata cccttgatta tcaatagcat aaccataacc
     3301 gatgaaaatg aaatatttct ttatatttca tattgttaag tgatacttaa ataaatttca
     3361 tcagtaaatc attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa
     3421 ccattcccat tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccctttt
     3481 ggctgcgtaa gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc
     3541 ttggcgtggt taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc
     3601 taagccagaa agaaaccgat aagctttggc agcttgtcct ttggttccct gccctgctgg
     3661 ggatctttgt attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct
     3721 ggcgcgaatg gttaacagat tactacctta atcgctggtt tgcagataaa aactactact
     3781 tcacgcagat ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag
     3841 acattctctt actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta
     3901 gcatgctgat cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg
     3961 ttggtggaac agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga
     4021 tcggtggaac tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa
     4081 aacaacgtag cgaagcgaca tttcgaacta atcttgtgca gcataataag caggcagaac
     4141 tgattgcgct aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata
     4201 ccattaaaga gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa
     4261 atatttattc gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta
     4321 gcgggcagat caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga
     4381 acaatttaag ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg
     4441 atcgcttgta tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt
     4501 gccaacatgc ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat
     4561 tagagaactt gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg
     4621 gtgcgggaaa aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg
     4681 atatttcttc tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct
     4741 tactgaaaga gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg
     4801 aagtactgca tcaggttggt ctggggaaat tggctgcgcg tattcatgag cacgatcgct
     4861 ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac
     4921 gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta
     4981 tacgcttact gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac
     5041 atcaacccgg tgtctggaac ctggctgatg atatttgtga cattagcacg gttttataac
     5101 aacaataaaa gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc
     5161 gtcattttat gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat
     5221 ttttttgaag agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat
     5281 tacaccagtg aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg
     5341 agaaccaacc ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat
     5401 attcgccctg agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt
     5461 gacggtatta gtgcaactaa taacctgaac ccagcgaatg agtccgatgc cagtagtgca
     5521 accaatatta gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg
     5581 acgctttatg acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat
     5641 gcaaagatca aacgcttcaa cgctgatgat agcaaggtta aattgggtta tcgcactacg
     5701 cgttcggact ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggc
     5761 tcttcaggaa gtacttatta ctccccagat tttaaaaaga acttttatac cttgtcgttt
     5821 aatcaggaac tcgctgataa cttcggcgtt accgccggtt tatcgcgccg ccagtctgat
     5881 atcacccgcg cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa
     5941 aacgttatcg atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac
     6001 gatttaacct taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag
     6061 tctgatcgcg aaatgggtaa taaatcctac ggtctggcat gggatatgga tacgcagctc
     6121 gcatgggcca aactgcgtac caccgttggt tgggatcata ttagtgatta tacccgtcac
     6181 gatcatgaca tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgc
     6241 acccgtggcg gattaggaca catttcccag gctgtagata attacacctt caaaacacgc
     6301 ctggactggc aaaaattcgc cgtgggtaat gtttcgcatc aaccctactt cggcgcggaa
     6361 tacatctatt ccgatgcgtg gactgaacgc cataaccagt ctgaatccta tgtgattaat
     6421 gctgcaggaa agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt
     6481 gacaactaca cgctgtatat ggcggatcac attagctggc ggaatgtgtc gttaatgccc
     6541 ggtgtgcgtt acgactatga caactatctg tcaaaccaca atatctcccc gcgctttatg
     6601 acggaatggg atatttttgc taatcaaacc tcaatgatta ccgccggtta taaccgttac
     6661 tatggcggga atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg
     6721 gtatcaggta ataaaaccct gacgcgttat cagaatttga aaacgcctta taacgatgaa
     6781 ctggcaatgg gattgcagca aaaaatcggt aagaacgtta ttgcacgcgc aaactatgtt
     6841 taccgtgaag cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc
     6901 accattactg aatataacaa cgatggcaaa accaaaacgc attcgttcag cctcagtttt
     6961 gaactggccg aacccctgca tatccgccag gtagatatta acccacaaat tgtctttagc
     7021 tataacaaga gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt
     7081 gataaccagg tggtttataa cggtaatctg gtctcttacg atagcgttcc agtggcagat
     7141 tttaataacc cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta
     7201 gtgtgggcga atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag
     7261 acaaatgcgc aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg
     7321 gatagcagcc tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa
     7381 aacctgacga tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca
     7441 acgaacaccg gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg
     7501 aaattttaat agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga
     7561 taattatgag aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc
     7621 ggctgattgc cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctgggcaatg
     7681 gcttgcgata tatgatttat ccgcatgctc atccaaagga tcaggtaaat ttatggctgc
     7741 aaattcatac cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag
     7801 aacatatgat gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg
     7861 agtcaatggg cctgcgtttt ggtcgcgatg ttaatgccta taccagctat gacgaaacgg
     7921 tgtatcaggt gagtttgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct
     7981 tcagtgaatg gagtaatgcc gcaacctttg aaaaactcga agtagacgct gaacgtggcg
     8041 taattactga ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc
     8101 gccctttcct gctggctaat acccgtaatt tagaccgtga acctatcggc ctgatggata
     8161 ctgtcgccac ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa
     8221 ataatatgac ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa
     8281 aggataattt aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa
     8341 aagccgaaaa ccacctgcgc tttaatatca ttaatgataa agaaaaccgg gtgaacggca
     8401 tcgcactcta ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttatcgaac
     8461 aagctgaatg gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt
     8521 cgggcgagtt gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt
     8581 atcagtcgct gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcgaatg
     8641 cattaatggc agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg
     8701 atgtcaaatc tacccgcctc acctggctga aaaatgcggt tgatcagcaa gctgaacgtg
     8761 atttacgtat gctgaccagt cgcctggcat ccagctcatt aaataatacg ccgttcttgt
     8821 cgccggaaga gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc
     8881 tggcggaaaa atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa
     8941 acaatgagct tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag
     9001 agtacgccaa caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag
     9061 tagacgctga cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat
     9121 cattaacact ttccaatggt gccagggtta ttctggcaaa atccgcgggt gaagagcaaa
     9181 agctacaaat tactgccgta tcgaataaag gcgatttaag tttccctgcg cagcaaaaat
     9241 cacttatcgc gctggcaaat aaagcagtaa gcggaagcgg cgttggtgag ctctcctctt
     9301 ccagcctgaa acgctggagt gcggaaaatt cggtaaccat gagcagtaaa gtcagtggca
     9361 tgaatacgtt gctctccgtt agcgcgcgga ctaataaccc tgaacctggt tttcagttga
     9421 ttaaccagcg aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg
     9481 ctcaaattca ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagcaga
     9541 tgtatgagac gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgtac
     9601 agtttactgc cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata
     9661 tcacgtttgt cattgtcggt aatgtctcag aagacaaact cgtggcgtta attacgcgtt
     9721 acttaggatc aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg
     9781 cgacggacaa cgcatcggtt actgtaaaag aacaaaatga acctgtggca cagttttcac
     9841 agtggaagcg ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg
     9901 cttttaacgt cgcactggca aaagatctac gtgttaatat tcgtgaacag gcatctggag
     9961 catacagcgt ttcttctcgc ctctcggttg atcctcaggc caaagatatc agtcatttgc
    10021 tggcttttac ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga
    10081 tggttaagcg tctggctaaa gggatcagtg agcaagaact gaatgaatac cagcaaaacg
    10141 ttcagcgcag cctcgatatc caacagcgta gcgttcaaca attagcgaac actattgtaa
    10201 atagtcttat tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac
    10261 aaatgacggt agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata
    10321 cttataccgg agtattattg ccaaaataat aacagccccg tcaacacatc gttg
//
LOCUS       DAZFFI010000150        10028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFFI010000150 DAZFFI010000000
VERSION     DAZFFI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.150"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2334..3167
                     /locus_tag="ACHJ12_004401"
     CDS             2334..3167
                     /locus_tag="ACHJ12_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoRI family type II restriction endonuclease"
                     /protein_id="HFO4992097.1"
                     /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKK
                     ALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYG
                     EWRVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSE
                     SHFPYVLFLEGSNFLTENISITRPDGRIVNLEYNSGILNRLDRLTAANYGMPINSNLC
                     INKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTS
                     K"
     gene            3197..4177
                     /locus_tag="ACHJ12_004402"
     CDS             3197..4177
                     /locus_tag="ACHJ12_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HFO4992098.1"
                     /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVV
                     YCNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKEN
                     GKKLVFDDISVSSFCGDGDFRSSESIDLLKKSDIVVTNPPFSLFREYLDQLIKYDKKF
                     LIIANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRI
                     ISPNNCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGV
                     MGVPITFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK"
     gene            complement(4673..5101)
                     /locus_tag="ACHJ12_004403"
     CDS             complement(4673..5101)
                     /locus_tag="ACHJ12_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO4992099.1"
                     /translation="MKRSGGTRYMYLSALITVAAALTACTPKTSLEQHTRHYVYASDD
                     GFDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLR
                     TTTTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
     gene            complement(5182..5619)
                     /locus_tag="ACHJ12_004404"
     CDS             complement(5182..5619)
                     /locus_tag="ACHJ12_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO4992100.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGR
                     REFETSELIRAYGELKRNETPERHTSGQAEKPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAEREQLQNEIARLRQALEQEKKRGFWSRLFGR"
     gene            6100..7845
                     /locus_tag="ACHJ12_004405"
     CDS             6100..7845
                     /locus_tag="ACHJ12_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFO4992101.1"
                     /translation="MSEGVTKINKLLEQARNAYPQNAEQIRDEASALLADLRNTGLIN
                     NPDINPAANALQEAVNSLQINIDLKNQTELAVSSASQDAVNAFREFMNNNKLSNFIDK
                     PADMIDLAIATSRWLHKPWDNTVVPKFSAEIEVKNKLVTYLDRGNTITADVVSKKNAL
                     AEVKSRAESQAKADAEAKARDELFAKAGVKPAPVYTSQMVAEANAALKAPDAMVLSQA
                     PGSVQIAMVGVGVWTAAGDVAGNISRWFAEALSKVSVPEVSPLLLRLSLGTLWFHSEP
                     AGQGSDIVPGRSLDAMFALNAQMLAGQGVKIEPGATSVNLPVRGQLVTSNGQLALDLL
                     KTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSRTILVNPAPQPPAPTDTGNNQ
                     PVPVTPVHTGTEVKPVEIPVTTITPVSDAGGFRDFIYWRPDATGTGVEPVYVMLSQDP
                     RKLPGKATGKGQGTGENWLNDANKGIGVPIPSRIADKLRGREFANFDAFRKALWTEVG
                     KDPELLKQFKNQNKVLVTKGKSSFVPESERVGERERFELHHIKRVTDGGAVYDIDNLR
                     VVTPKHHIEIHRGNK"
     gene            7846..8103
                     /locus_tag="ACHJ12_004406"
     CDS             7846..8103
                     /locus_tag="ACHJ12_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO4992102.1"
                     /translation="MEKKTISDYTEAEFLEFVRKICRAEGETEADDDKLVDEFRRLAE
                     HPDGSDLIYYPRDDREDSPEGIVKEVKEWRAKNGKPGFKQG"
     gene            8191..8338
                     /locus_tag="ACHJ12_004407"
                     /pseudo
     CDS             8191..8338
                     /locus_tag="ACHJ12_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
     gene            complement(8536..8694)
                     /locus_tag="ACHJ12_004408"
     CDS             complement(8536..8694)
                     /locus_tag="ACHJ12_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4992103.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAVGNLVSRAMM
                     AQMVLQSS"
     gene            8994..9212
                     /locus_tag="ACHJ12_004409"
     CDS             8994..9212
                     /locus_tag="ACHJ12_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992104.1"
                     /translation="MFFPGLFPGSVDNRVSVFEKGESARRSRTTERSESVSEEAEKHL
                     DVHFLLTHLCGISHPAWCTFHTILTDAT"
     gene            complement(9359..9553)
                     /locus_tag="ACHJ12_004410"
     CDS             complement(9359..9553)
                     /locus_tag="ACHJ12_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4992105.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELGNSIQTHFEAQYGTEP"
BASE COUNT         2705 a         2117 c         2466 g         2740 t
ORIGIN      
        1 atgatgcgct tcattatgcc aaaggagaaa atataggttg gtgtatagcc agagctgaat
       61 attgtaaaaa gacaggccgg ttttaattct ggcggggaat caaaggggcg ccccctttga
      121 tacgtcgacg tgcctccaga atttaccctc aaaacgcctc tgtaactgac gcgcatcaaa
      181 agtataacta agatataatt aacatataac caatatataa ctaacggttc accaacggat
      241 cgcatgtagt agacgttaca taaaaaatcc tgaaatggag gattcgtcat aaagagattc
      301 caatcctgtt ttttagcgtt ctgatgcagg acagggcaaa caccgcaaaa atgatgaaaa
      361 actgattaac catactgtct gtattctgaa tgaattacga agcatagcga taaattaaaa
      421 aatccccctg gattacaggg ggaagccatc agagtttgag taatgagaaa tattattctg
      481 gagagagcac cacaaatgcg ctcttttcca atctgccgta accatcggca aaagacaatc
      541 aggacaggcg aaaaaatccc cctatatggc aagggggtgt caatgaggac atggcaattg
      601 tgagttttgt gtgtttttta tgtgttgcag tttgcaacgt cagaaatttg ccgtagttcg
      661 tcatgagaga caacaaaaaa atcccccgtg actggtggag gccagggcaa ggggggtagc
      721 aaatgcctga ataattttcg tagttttgat atacccatcg ttgcaaccgc aacgggatga
      781 atttgccgca gttgtgcgcg aaaaacaatc ctgattccgg gacaggatgt gtaatcgtat
      841 aaccgcgcac gcacggctat acgattacca cctggtcagg gctttgcccc gacaccctcg
      901 acaccgttcc cgcgatacga tagaacatca gggcgcgttt ccgggtaaac gcatgttaat
      961 gaccgggaat tcattgatca gggaccggag acgcgctaca aaactcctgc gcgtttctgg
     1021 catcaactgg ccatggggat ggtttggcag tagcagcaag cgccagaaac gcgctcaaaa
     1081 cgcctctggc ggcaaaacca ttgcccgatg caaaaaagtg cgttagaaag cgtctgagcg
     1141 cgtttcctgc cacctgaacg tcatctccgg gattcgtgct cactccatca aataacaaat
     1201 aaacttcaaa gtctgataat gggttccccc tgtaatccag gggatttttt tcgctcactc
     1261 attaccgcac aactcgacat catgccggag acacgctaaa acacacctgc gcgtttctgg
     1321 tgcaaagagt agttcgtagg gatgattaca gtcaggtatg taccagaatc gcgccctgaa
     1381 cacgcctgaa cgcctcaaag cgaaaaaaca accgaagcca ttgcctcatg taaaaaatcg
     1441 cgttacaaag cctctgagcg cgtttctggc gtcttttttg ttgctgtgtc gctatactcg
     1501 ttgtcagatt cgcgctcaat catcactgat taatctgcgc ttatccctcg actgatcacg
     1561 ctggtatttt ttgacaaccg gttcccatga aaacgccaga cgggaaccgt catccgcacc
     1621 ctctgacatg ctggggatta aaaatccccg tgcagcactg ccggcgttgc ataaaattcc
     1681 gttctgcggt gagtattgtt taatcaaaaa tccccgcagt tcttccggaa aaacgcgagt
     1741 ctgttctcat gcctgaaatg cccacacact acgcaaaacc aactttttgc tgatttgctt
     1801 tttaaatcat gtcgttatgg ttctaaaaat atattttctt attcaggtgt atacaaaagc
     1861 gagtcgtcgg cgactactgg atgattcggc aacgagtcgt cggtgattta tcgatgactc
     1921 aacacactgt ctctctggtg ttcagagaag gcgagtcgcc agagagtcgc gaggaaaata
     1981 ctggatgact cgctgatgac tcccggggtg aatcgctcac gactcgtctg taactactgt
     2041 ctgatttctt ctcatcaggg ctgaaaacgt ccgaaaatgc gccttatggg catcaggcgc
     2101 gcccaaaccg atcgccattc agtttcatgc atagtcatgc agtgccatga aaacaatcct
     2161 gacgcatttt tccggtttac ccgggggaaa acatctattt ttgcggtttt tgcgccagaa
     2221 tcgcgctgag cgcgtttctg cggtgcgtac aattaaggga ttatggtaaa tcaaacgtat
     2281 gttaatctat cgacatatgt aactttataa aataacagtg gaaacatgga ttcatgtcta
     2341 ataaaaaaca gtcaaatagg ctaactgaac aacataagtt atctcaaggt gtaattggga
     2401 tttttgggga ttatgcaaaa gctcatgatc tcgctgttgg tgaggtttca aaattagtaa
     2461 agaaagctct tagcaacgaa taccctcaat tatcatttcg atatagagat agtataaaga
     2521 aaacagaaat aaatgaagct ttaaaaaaaa ttgaccctga tcttggcggt actttatttg
     2581 tttcaaattc cagcatcaaa cctgatggtg gaattgtaga ggtcaaagat gattatggtg
     2641 aatggagagt tgtacttgtt gctgaagcca aacaccaagg taaagatatt ataaatataa
     2701 ggaatggttt gttagttggg aaaagaggag atcaagattt aatggctgct ggtaatgcta
     2761 tcgaaagatc tcataagaat atatcagaga tagcgaattt tatgctctct gagagccact
     2821 ttccttacgt ccttttctta gaggggtcta actttttaac agaaaatatc tcaataacaa
     2881 gaccagatgg aaggattgtt aatcttgagt ataattctgg tatattaaat aggttagatc
     2941 gactaactgc agctaattat ggaatgccta taaatagtaa tctatgtatt aacaaatttg
     3001 taaaccataa agacaaaagc attatgctac aagcagcatc tatatatact caaggagatg
     3061 ggagggagtg ggattcgaaa atcatgtttg aaataatgtt tgatatatca acaacttcgc
     3121 tcagagtgtt ggggcgtgac ttgtttgaac agcttacatc taagtgatat tttttatttt
     3181 aataaggttt taattaatgg ctagaaatgc aacaaacaag ttactgcaca aagctaaaaa
     3241 atcgaaaagc gacgaatttt acactcagta ttgtgatatt gagaacgaac tgcaatacta
     3301 cagagagcac ttctctgata aggttgttta ttgcaattgt gatgatccta gagtaagcaa
     3361 tttctttaaa tattttgcag tgaattttga taatcttggc ttgaaaaagt taatagcatc
     3421 ttgctatgta gagaataaag aaggtttttc tagtagcgaa gccgcgaaga acggatttta
     3481 ctatgaatat cataaagaaa atggaaagaa attagttttt gatgatatta gtgtttcttc
     3541 tttctgtggc gatggcgatt ttcgcagttc ggagagcatt gatctgctaa aaaaatcaga
     3601 tattgttgtt acgaatcctc cattctcgtt atttagagag tatcttgatc aactaattaa
     3661 gtatgataag aaattcctta taattgctaa tgttaattca ataacatata aagaggtgtt
     3721 taatctaatt aaggaaaata agatttggct tggggttcat ctcgggagag gtgtttctgg
     3781 atttattgtt ccagagcatt atgaattata tggtactgag gcgagaattg attctaatgg
     3841 taatagaatt atctcgccaa acaactgctt atggctaact aacctggatg tctttattag
     3901 gcataaagac ttgcctctta caagaaaata ttttgggaat gaaagttcat atccaaaata
     3961 tgataattat gatgctataa atgtaaacaa aacaaaggat attccattag attacaatgg
     4021 ggttatgggg gttcctatca cattcttgca taagtttaac cctgagcaat ttgagttaat
     4081 aaaatttaga aagggtgttg atgaaaaaga tttgtctata aatggtaaat gtccttattt
     4141 cagaattttg ataaaaaaca aacgattaca aaagtaattg atgtttgtta gttttttctt
     4201 gagatcatta gcttcgtcgt aagcttcggt accagaaacg aaaaaaccac ctctccaggt
     4261 ggttttttcg aaggttcagc aaaccggcaa gtttctgaac catggtaact ggcttaagca
     4321 gggcgcagat accaaataca gtcctttctg tgtagccgta gttaggctat cacttcaata
     4381 atctgtaata gggagccgca aacggaaatt ttaccacgct aagagaattg agttgaacga
     4441 gtagttacat tcaggccaac taatttactg aacataaagg ctgtacgaaa tggcctaact
     4501 gtccgacaac cgtttggctt acttctgtac atccccttgc gcagatgtac agaattgtac
     4561 gatttcggat acaaaaactg ctattgtccc gccttctggc aatttccaaa agttgtttgg
     4621 ctaaaaaata gtcttaacgt ggtttgtttt ccgaattcca agtggcctcc acttactgtc
     4681 ttccctcgta gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ttcatttttt
     4741 ccggtgatgg catatcactg ttcgtgtatt ttctgcccgc aaaggtggtt gtggttctca
     4801 gtgactgaag gaattcttca gagtggaatt cttttatgcg ttgctgtgcc gtttctgctg
     4861 atactccggc tgctttgtct ttcaccccca tctcccggaa ctgctgaaag aacggaatca
     4921 tcatccgtgt ggtgtcagct ttcttggtgt agaagttagg atcaaaccca tcatctgaag
     4981 cataaacata atgccgggta tgttgttcca ggctggtttt tggggtacag gctgtgaggg
     5041 ctgctgccac agttatcagg gcgctaagat acatatagcg tgtgccgcca gaacgtttca
     5101 tgcgagtctc cttaccttaa agtatttatt ttcggtgagt tattatctga tggctatctt
     5161 atcatcagtc tctgagcgcg ttcagcgacc gaacaacctg gaccagaatc cccgtttctt
     5221 ttcctgctcc agcgcctgcc tgagacgggc tatctcattc tgtagctgtt cccgttctgc
     5281 ttcctggcgc cggcgctccg tatcctgggc ttgtttatcc tcaagcatca gcgtcaggca
     5341 ttgtttcagc tcgttcagct cccggagaat gcgttctgtc tgctgatcat gaggtttttc
     5401 tgcctgtccc gaagtgtgcc tttctggtgt ctcattccgc tttaattcgc catatgcccg
     5461 gatcagttca ctggtttcaa actcccgtcg tccgtcctgg cttgtacggt aactgacaag
     5521 gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cccgtcattt tcagtgcgtc
     5581 ccggagtgtg tgccatgcca taaagtgaca gtgtcccata ggtgtctcat atcatggttt
     5641 cagtaaaaca tactgaggcc ggagaacggt aatgtttgtg ctggtttttg tggcatcggg
     5701 cgggaatagc tcgtggtgtg gggggctgtt tttttgaacg ttttcacaaa aatgaaaatc
     5761 cacagggttg acagggaaaa cgaggcggcg taattttata ctgtatataa aaccagtggt
     5821 tgtatataca gtatttgttt ttaactgatt gtttttaatg tcaaagaggt ttttataatg
     5881 gaaacgcctg ttgcgtatta ttcagacggt gtgccttatg actcttctgg taatgtaatt
     5941 attactatta gtaacggagt ctcaggttct gtaggagcta attctaaatc tccatctcct
     6001 gtttttgtta actggcctgg tatgggaggg cctcatggtg ggggaggtgg gattattgat
     6061 tataatccga ataaattgcc ctttactggt ttatggaaga tgagtgaggg agtaacgaaa
     6121 attaataagc ttctggagca ggccagaaat gcgtatccgc agaatgctga acaaatcagg
     6181 gatgaggcca gcgctttact cgctgactta agaaatacag gacttatcaa taatcctgat
     6241 atcaatccgg cggcgaatgc tctgcaggaa gcggttaatt ctctgcaaat caatattgat
     6301 ttgaaaaacc agacggagct ggcagtcagc agcgcgtcgc aggatgccgt gaatgctttt
     6361 cgtgaattta tgaataacaa taaactctcg aactttatag ataaacctgc cgatatgatt
     6421 gatttggcga tagcgacaag tcgctggctt cataagccgt gggacaatac cgttgtgcct
     6481 aaattcagtg cggagattga ggtaaaaaat aaactggtca cctaccttga ccggggaaac
     6541 acgataacgg ctgatgtggt cagtaaaaaa aatgcgctgg cagaggtaaa aagccgcgca
     6601 gaatcccagg caaaggcaga tgccgaggcc aaagcaagag atgagctgtt tgcgaaagct
     6661 ggcgtgaagc ctgctccggt gtatacctcg cagatggtag cagaagcaaa cgcagccctc
     6721 aaagcccctg acgctatggt gctgagtcag gcgccgggct cggtacagat agcaatggtc
     6781 ggtgtcgggg tatggacagc cgccggagat gtggcgggaa atattagcag gtggtttgct
     6841 gaggcgcttt caaaagtgtc tgtgccggaa gtaagtccgc tgctactacg tctttctctg
     6901 ggcactctgt ggttccattc agagccagcg ggccagggca gtgacatcgt acctggacga
     6961 agtctggacg ctatgtttgc actgaacgca cagatgctgg ctggtcaggg ggttaaaatc
     7021 gaaccgggtg caactagcgt gaacctgccg gtacgtggtc agttggtgac tagtaacggg
     7081 cagttggctc tggatttgct gaaaacaggg aatgaaagta tcccggctgc ggttcctgtt
     7141 cttaatgctg ttcgtgatac agcaacagga ctggataaaa ttacgctgcc agcagtagtc
     7201 ggtgcacctt cccggacgat tctggtaaat ccggccccac aacctccggc accaacagat
     7261 acaggaaata atcaacctgt tccggttacc ccagtgcata caggtacgga ggtaaaaccg
     7321 gttgaaattc cggtaacgac gattactcct gtctctgatg ccggtggatt tcgagatttt
     7381 atttactggc ggcctgacgc aaccgggacg ggcgtggagc cggtctatgt gatgctaagc
     7441 caggatcccc ggaagctgcc gggaaaagcg acgggcaaag ggcaggggac cggagaaaac
     7501 tggcttaatg atgctaacaa aggtatcggt gttccgatac cgagccgaat tgcggacaag
     7561 ttgcgcgggc gtgagtttgc gaacttcgat gcgttccgta aggcgttgtg gacggaagtg
     7621 gggaaagatc cggagttact gaagcagttt aaaaatcaga ataaggttct ggttacaaaa
     7681 ggtaaatctt cgtttgttcc tgaatcagag cgtgtaggtg aacgagaacg tttcgaactg
     7741 caccatatca agcgcgttac ggatggtggc gctgtttatg atatcgataa tctcagggtc
     7801 gtgacaccca agcatcatat tgaaattcac agaggaaata agtaaatgga aaagaaaacg
     7861 atttctgact ataccgaggc ggagtttctg gagttcgtca gaaaaatctg ccgggctgaa
     7921 ggtgaaactg aggctgatga cgataaatta gtggatgaat ttcgtcgatt agctgaacac
     7981 cctgatggtt ctgatcttat ttattatcca agggatgacc gtgaagacag tcctgaaggt
     8041 atcgtaaaag aagtgaagga atggcgagcc aaaaacggca agccggggtt taaacagggc
     8101 tgaggatctt tgctaaagga ttagctgaaa atatgaatgc cagttcttta aggatgaatg
     8161 gctggtattc gttcacgaca gggagatgtt atgaaaaaaa agataggatt gcttttgatt
     8221 ctgatggttg ctgctttatt tctggcagcc tgtcagacga actatatccg ggatgttcag
     8281 gggggggact gtcgcgccat cctcttcttc tgaactgacg gggatcgcgg ttcaatagaa
     8341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg
     8401 aaaaaaccac cctggcaggt ggtttttcga aggttagcta atcctggcag attatctaac
     8461 cgaggtaatc tggctaaagc agagcgcaga taccaaatac tgtccttcca gtgtagccgc
     8521 agtcgggcca taacttcaag aactctgtag caccatttgc gccatcatcg ctctgctaac
     8581 caagttacca acggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag
     8641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8701 cagcttggag cgaacgacct acaccaagcc gagataccaa cagcgtgagc tatgagaaag
     8761 cgccacgctt cccgaaggga gaaaggcgga caggtatctg gtaagtggca gggtcggaac
     8821 aggagagcgc acgagggagc ttccgggggg aaacgcctgg tatctttata gtcctgtcgg
     8881 gtttcgccac ctctgatttg agcgtcgttt tttgtgatgc tcgtcagggg ggcggagcct
     8941 atgaaaaaac gccactggcg cggccttgtt ccgggttttc gttttttgcg cacatgttct
     9001 ttcctggttt attcccgggt tctgtggata accgggttag cgtttttgag aaaggggagt
     9061 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc
     9121 acctggacgt gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggtgca
     9181 cttttcatac aattctcact gatgccacat gattaagcca gtacacactc cgctatcgct
     9241 acgtgactgg gtcatggctg cgccccgaca cccgctaaaa cccgctgacg cgccctgacg
     9301 agcttgtctg ctggcgaaaa cactcacgga caagccgcga ccgctttcgc cagccccttt
     9361 atggttccgt gccgtactgc gcttcaaagt gtgtctgtat gctgttaccg agttcttccg
     9421 ccagttcgtg cagtcgttcg cacatggcgg cctgttcgtc tgcatccagt gcatccagtt
     9481 tttcgagcaa cgtcaggctc tggcttttga tgaatcgcgc catgttgagc gcggtctgct
     9541 gctgtttgtt cacgttctcc cttctccgtt actggtgttt acgtccggct gtgcgtcacc
     9601 tggtcatcac gccaggtgac gtcactgaaa attatcacac aagccccgtc gtgtgtgggc
     9661 tgcgcgacat taacaccccg tgacgcttat tttttgctga atatgtgcgc gattttctgt
     9721 ctgcgttgtg tgcagttgtc gtcataaaca tgaacgttcc gccggttcct gatgtgcgtc
     9781 actttgacgt tttgacaaag tgacgcacgg gaggtgcttg cgcaaacccg cgaataacgg
     9841 cgtttaccgc gtgatttttt aacatctgac aaagtgaccc ggcgtgatgg ccagttctgg
     9901 ccagagacgt tcgcggggtg tcgggcgcag ccatgaccag atggtgtttg tttgatggcg
     9961 cgtgcgcggt taaacaaacg cacatcctgt cccgtttttc gctcggtttt tattgattac
    10021 cggacaga
//
LOCUS       DAZFFI010000151        10012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFFI010000151 DAZFFI010000000
VERSION     DAZFFI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.151"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..316
                     /gene="hokA"
                     /locus_tag="ACHJ12_004411"
     CDS             164..316
                     /gene="hokA"
                     /locus_tag="ACHJ12_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO4992106.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(305..364)
                     /locus_tag="ACHJ12_004412"
     CDS             complement(305..364)
                     /locus_tag="ACHJ12_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992107.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(504..716)
                     /gene="cspA"
                     /locus_tag="ACHJ12_004413"
     CDS             complement(504..716)
                     /gene="cspA"
                     /locus_tag="ACHJ12_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4992108.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(997..1287)
                     /gene="yiaG"
                     /locus_tag="ACHJ12_004414"
     CDS             complement(997..1287)
                     /gene="yiaG"
                     /locus_tag="ACHJ12_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4992109.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1721..2431
                     /gene="yiaF"
                     /locus_tag="ACHJ12_004415"
     CDS             1721..2431
                     /gene="yiaF"
                     /locus_tag="ACHJ12_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4992110.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2481..3455)
                     /gene="ghrB"
                     /locus_tag="ACHJ12_004416"
     CDS             complement(2481..3455)
                     /gene="ghrB"
                     /locus_tag="ACHJ12_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4992111.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3559..4218)
                     /gene="yiaD"
                     /locus_tag="ACHJ12_004417"
     CDS             complement(3559..4218)
                     /gene="yiaD"
                     /locus_tag="ACHJ12_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4992112.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4371..6704
                     /gene="bisC"
                     /locus_tag="ACHJ12_004418"
     CDS             4371..6704
                     /gene="bisC"
                     /locus_tag="ACHJ12_004418"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4992113.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQMNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6673..7113)
                     /gene="yiaC"
                     /locus_tag="ACHJ12_004419"
     CDS             complement(6673..7113)
                     /gene="yiaC"
                     /locus_tag="ACHJ12_004419"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4992114.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7110..7673)
                     /gene="tag"
                     /locus_tag="ACHJ12_004420"
     CDS             complement(7110..7673)
                     /gene="tag"
                     /locus_tag="ACHJ12_004420"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4992115.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7831..8529
                     /gene="yhjY"
                     /locus_tag="ACHJ12_004421"
     CDS             7831..8529
                     /gene="yhjY"
                     /locus_tag="ACHJ12_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4992116.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8758..9960
                     /gene="yhjX"
                     /locus_tag="ACHJ12_004422"
     CDS             8758..9960
                     /gene="yhjX"
                     /locus_tag="ACHJ12_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4992117.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTRLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2388 a         2436 c         2577 g         2611 t
ORIGIN      
        1 gctagaatag ccacgggtgc ttgagactgt ttgtctcagg tattcaccga aaggcagaca
       61 gagaaaagcc ccacctgact ataaatcaaa gtgaggctac cctatgcctg aacaccataa
      121 ggttagcctc ttactcgttg gaaatcaaca cagggggctg ggaatgccgc agaaatatag
      181 attactttct ttaatagtga tttgtttcac gcttttattt ttcacctgga tgataagaga
      241 ttcactgtgt gaattgcata ttaaacaggg gagttatgag ctggcggcat ttttagcctg
      301 caatttaaaa gagtaagagt cttcggcggg aagttattcc cgcctttctt acggcgttgc
      361 gcattctcat tgcacccaaa tttatttttc acaaaaataa tactagattt tattacgcga
      421 tcgattattt atttcctgaa aaacaaataa aaaaatcccc gccaaatggc agggatctta
      481 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      541 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      601 ttctggatag cagagaagtg tacgaacacg tctttagagc catcgtcagg agtgatgaag
      661 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      721 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      781 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      841 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      901 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      961 atcaggaccg taaacagcaa taaagtggat aaaagtctat tccatcaact gcttacttaa
     1021 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1081 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1141 gatttttaac cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1201 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1261 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1321 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1381 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1441 cgatctgata aatgccgata tatctcttac cactcctgtt ttgtaaagat ttcgaaaatc
     1501 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggcac tatcattttt
     1561 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1621 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1681 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1741 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1801 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1861 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1921 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1981 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     2041 aagcggtccg ctgcgtgaaa tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2101 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc
     2161 ggtctttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2221 gattccggct gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2281 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2341 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2401 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2461 ttacttcacg cagtcgcggc ttagtccgcg acgtgcggat tcacacagtt cttctcaacc
     2521 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2581 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2641 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2701 ttctgcaatg cagcaatcag tgcattttca tcaaccaccg ggccacgtcc ggcattaatg
     2761 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2821 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2881 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2941 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccataccgat ccgtcccatc
     3001 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3061 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3121 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3181 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3241 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatcaac attctcgttt
     3301 gaacccagta aaccttcagc ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg
     3361 ctgaggtttg ccacctggtg aacggtgaaa tgctcttgca ggcgttgcag taaatcatca
     3421 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3481 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3541 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3601 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3661 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg
     3721 ctgggagaga cgcatgttca ggtcgtgacc acccgtgctg tcggtataac caatcacgtt
     3781 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3841 cgcaggtttc agggtcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3901 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3961 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     4021 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4081 gcccacgaga gagcccagac ctgcgccgat agcagattta cctgcttcgc gttcgccggt
     4141 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4201 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4261 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4321 taagtaatat ttaactgctc aatacatcta acctttcagg agttttcggt ttggccaact
     4381 catcctcacg atattccgtt ctgactgccg cccattgggg gcccatgctg gttgaaaccg
     4441 acggcgaaac cgtgtttagc tcgcatggcg cgttagccac aggaatggaa aactccttgc
     4501 agagcgcggt tcgcgaccag gttcacagca atacacgggt acgatttcca atggtgcgca
     4561 aaggctttct tgcgtcaccg gaaaatccgc aaggcattcg tgggcaggat gaatttgttc
     4621 gcgtgagttg ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg
     4681 cttatggtcc ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc
     4741 ataaggcctc gacattatta caacgctata tggcgctggc aggcggttat accgggcatc
     4801 tgggggatta ttcgactggt gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg
     4861 aagtttatca acagcaaacc agttggccgc tggtgctgga acatagcgat gtcgtggtgt
     4921 tgtggagtgc taacccactc aatacgctga aaattgcgtg gaatgcatct gatgagcagg
     4981 ggctttctta cttttccgca ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa
     5041 tgcgatcgga aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg
     5101 gcaccgatgt tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg
     5161 acgaagcgtt tctgacgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg
     5221 gcgagagtga cggaatagcg aaaaacgccg aatgggcggc agagatttgt ggggttaacg
     5281 cagcgaaaat ccgcgagctg gcggctcttt tccaccaaaa cacgaccatg ctgatggctg
     5341 gctggggaat gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg
     5401 cggcaatgtt ggggcaaatc ggcacacccg gcggcggttt tggtctttct tatcattttg
     5461 ccaatggtgg taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc
     5521 cgggtggctg cgatgcggtg gataaaatcc ctgttgcccg cattgttgaa gcactggaaa
     5581 accctggtgg cgcatatcaa cacaacggta tgaaccgaca tttcccggat attcgtttta
     5641 tctggtgggc gggcggtgcc aactttactc atcatcagga taccaatcgt ctgatccgtg
     5701 cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac
     5761 acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg
     5821 gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag
     5881 cgcgtaatga ttttgatgtt tttgccgagt taagtgaacg ctgggagaag ggcggttatg
     5941 cacgttttac ggaaggaaaa agtgagctgc aatggctgga aacgttttat aacgttgccc
     6001 ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca
     6061 accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt
     6121 tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct
     6181 cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc
     6241 cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg
     6301 cgcaccgcct gcacagccag atgaattaca gttctctgcg cgaattgtac gcggtagcaa
     6361 accgtgagcc tgtcaccatt catcctgacg atgctcaggc gcgcggcatt caagatggcg
     6421 atactgttcg gctgtggaac gcacgcgggc aaattcttgc cggagcggtc attagcgagg
     6481 gaattaaacc tggcgtgatt tgtattcacg aaggggcatg gccggatttg gatttaaccg
     6541 ctgacggtat ttgtaaaaac ggcgcagtca acgtgctgac caaagatctc cccagctcgc
     6601 ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc
     6661 cggaactgac acttacagcg tttgaaccac cggccagctc ataatccagg tgggtagttg
     6721 ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat
     6781 cgccggttga tttttttgat aaacctccag catcagatgg ggatagcgct gctgcacata
     6841 ctgcatcagc gccttaccaa taccgcgtct gacggccttc ggtgcgacaa acattgctgc
     6901 cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtcct cttcccagac
     6961 ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta
     7021 attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc
     7081 ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag
     7141 ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg
     7201 ccgacaaact taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta
     7261 gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac
     7321 gaccagacaa agtcggcaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca
     7381 ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt
     7441 ctttcgacat cctcttcctg cattgcggca accttcaccg gatcgaactg atgaaagcag
     7501 gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct
     7561 tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccactcatta
     7621 tcatggtagg caatataaag cgggtcctga ctaacccagc cgcaacgttc catactttcc
     7681 ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag
     7741 tcaatattta atacagggct actatgcccg atatccttca tacaatgaca ataatattgc
     7801 cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg
     7861 cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc
     7921 tggcaacaag aatatatcgt tgatacgcaa cccggacttt ccacagagcg ttacacctgg
     7981 gatagtgatc atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa
     8041 agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt
     8101 atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca
     8161 ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg
     8221 ctgaccgatc cgttatggca tgccagcgtg agtaccttag gctggcgtgt tgactcccgg
     8281 cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc
     8341 tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg
     8401 gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta tgccgcgcta
     8461 actcaggcag aaaataccac taataatagc gactatctgt atacgatggg ggttagcgcc
     8521 agattttaac gtaacagtca caattgaaac cattgaataa caacagttgt ggcgatagtg
     8581 ggtgctaact taccaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac
     8641 taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaagtgc
     8701 acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg
     8761 acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtacaat cattacccag
     8821 tttgcgctgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc cgccaagctg
     8881 gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt
     8941 tcatcttctg ttgcgggcaa attacaggaa cgttttggcg ttaaacgcgt caccatggct
     9001 tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attctgacaa cctgatgatg
     9061 ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg
     9121 ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatatccgc gttcgctatc
     9181 ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcggct gctggaaacg
     9241 gtgggtctgg aaaaaacctt tgtgatttgg ggagcgattg cgctggtgat gattgttttc
     9301 ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg
     9361 gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta
     9421 atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc
     9481 caaagtctgg cacaccttga tgtggtttcc gcagctaatg cagtcactgt tatttccatc
     9541 gccaaccttt caggtcgtct ggttctgggg atactgtctg acaaaatcgc ccgtatccgt
     9601 gttatcacca ttggtcaggt gatttcgcta gtgggtatgg cggccctgct gtttgcacca
     9661 ctgaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact
     9721 attaccgtct ttccgtcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac
     9781 tacggtgtga tttacctcgg tttcggtatc ggtagcattt ttggttcgat tatcgcctca
     9841 ctgtttggcg gtttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg
     9901 gcgctttcta cgacgattcg tcagccagag cagaaaatgt tgcgtgaggc gcatatttag
     9961 aaatatgttg ctactgaaat aaatacgagt agggagactt caaagtctcc ct
//
LOCUS       DAZFFI010000152         9976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFFI010000152 DAZFFI010000000
VERSION     DAZFFI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.152"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            139..711
                     /gene="lpfA-O113"
                     /locus_tag="ACHJ12_004423"
     CDS             139..711
                     /gene="lpfA-O113"
                     /locus_tag="ACHJ12_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO4992118.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            759..1490
                     /gene="lpfB-O113"
                     /locus_tag="ACHJ12_004424"
     CDS             759..1490
                     /gene="lpfB-O113"
                     /locus_tag="ACHJ12_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO4992119.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1515..4037
                     /gene="lpfC-O113"
                     /locus_tag="ACHJ12_004425"
     CDS             1515..4037
                     /gene="lpfC-O113"
                     /locus_tag="ACHJ12_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO4992120.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            4048..5121
                     /gene="lpfD-O113"
                     /locus_tag="ACHJ12_004426"
     CDS             4048..5121
                     /gene="lpfD-O113"
                     /locus_tag="ACHJ12_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO4992121.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5369..6409
                     /gene="pstS"
                     /locus_tag="ACHJ12_004427"
     CDS             5369..6409
                     /gene="pstS"
                     /locus_tag="ACHJ12_004427"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4992122.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6496..7455
                     /gene="pstC"
                     /locus_tag="ACHJ12_004428"
     CDS             6496..7455
                     /gene="pstC"
                     /locus_tag="ACHJ12_004428"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4992123.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7455..8345
                     /gene="pstA"
                     /locus_tag="ACHJ12_004429"
     CDS             7455..8345
                     /gene="pstA"
                     /locus_tag="ACHJ12_004429"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4992124.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8436..9209
                     /gene="pstB"
                     /locus_tag="ACHJ12_004430"
     CDS             8436..9209
                     /gene="pstB"
                     /locus_tag="ACHJ12_004430"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4992125.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9224..9949
                     /gene="phoU"
                     /locus_tag="ACHJ12_004431"
     CDS             9224..9949
                     /gene="phoU"
                     /locus_tag="ACHJ12_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4992126.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2575 a         2281 c         2526 g         2594 t
ORIGIN      
        1 atattttatt tttgcgtaat taatattatt tttagataaa gccgtctctt tggtgtacta
       61 ttcctttgaa tcaaaaatac acttattaat ataagtgatg attcatggta aaggatatat
      121 tatatcaatg gagtttttat gaagcgtaat attataggcg gtgcattcac tctggcatct
      181 ctaatgctgg ccgggcatgc actggcagaa gatggtgttg ttcacttcgt cggtgaaatt
      241 gtcgacacta cttgtgaagt tacctccgat acagccgatc aaattgtccc actgggtaaa
      301 gtcagtaaaa atgcattttc aggtgtaggt agtctggcgt cgccacagaa gttcagtatt
      361 aagcttgaaa attgcccggc aacgtacact caagcagccg ttcgttttga tggtacagaa
      421 gcgcctggcg gcgacggcga cctgaaagtg ggtacgccgc ttacagcagg caaccctggt
      481 gattttaccg gtacaggaca agcgattgcg gcaaccggcg ttggtattcg tatttttaac
      541 cagtccgata attcgcaggt taaactttat aacgactctg cttataccgc tatcgatgct
      601 gaaggcaagg ctgaaatgaa gtttattgca cgctatgtgg caaccaatgc gaccgtaacg
      661 gctggtacgg cgaacgctga ttcacaattt actgtcgaat ataagaaata atttatcaag
      721 ggccttttta ttcagttggc tttggccagg gagtcgttat gtccagaaaa agttgtttaa
      781 tcaggctctt taaaacaaca gtttcatgta agttatttac cgcgattata ctcagcgttt
      841 ttttaggcca accagcatta acttatgcag gcgttgtcat tggtggtacg cgggtggttt
      901 acttaagtaa taatgctgat aaatctattt ctgtatttag caaagaagaa aaaatacctt
      961 atctcatcca ggcttgggta gatccattta ataaagagga taaaagcaaa gcgccgttta
     1021 ccgttattcc gccagtatca cgattagaac cttcgcaaga aaaaatatta cgcattattc
     1081 acaccaaagg tgtgtcattg cctgatgatc gcgagtcagt cttctggctg aatattaaaa
     1141 atattccgcc atcagcgagc aacaaagcga cgaactcgct ggaaatagca gtaaaaacgc
     1201 gtatcaagct gttctggcga ccagcaaata tccgccttat tcccgaagat gctgcaccga
     1261 aagtgaaatg gcgccgggaa gggcgaaatc tgatagcaga gaatcccaac ccaattcata
     1321 tcagtgtgat ggatgtcatc gttgatggcc atgatgtgcc cctcaatatg atccgtcctt
     1381 ttgaaacgct cacgttacct ttacctgcta attctgcggg aggccaaatg acctggcgat
     1441 ttattaatga ttacggcgca gtcagtgacc cgataaaaat gacgctgtaa gcaagggata
     1501 aggataggcg tattatgatg acgaccagaa tagtggttgg cctcacggca gggacgtgtc
     1561 tgattttctc gcaaaacctg atggccgagg tcagtgtatt caatccggcg cttctggaaa
     1621 tcgaccatca atccggagtc gatattcgcc agtttaatcg ggcaaacctg atgcccccag
     1681 gtgtttatag cgttgatatt tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat
     1741 ttgttcagga taatccagat gctgatctgc acgcttgctt tattgccatt aaaaaaacac
     1801 tgtcctcctt tggcataaaa gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg
     1861 tttgcctcga tcctgctcca cgtattgaag gctcatcctg gcagtttgac agtgataaat
     1921 tgcagctgaa tatatccatt ccccaaatct acatggacgc gatggcttat gattacatca
     1981 gccccacgcg ttgggatgag gggattaacg cgctcaccat caactacgat ttttctggtt
     2041 cacatacact acgttcagat tatggttcac aagagacaga taccagttat ctcaatctgc
     2101 gcaatggact gaatattgga ccgtggcggc tacgtaatta cagtacttta aacaccagcg
     2161 atggccgtgc ggaatacaac tccattagta cctggataca gcgcgatatt gccgcgttaa
     2221 gaagccagat tatgattggt gatacgtgga cggcgagcga tattttcgac agtacgcaaa
     2281 ttcgcggcgc gcgtttgtat actgataacg atatgctacc cgccagccag aatggctttg
     2341 ctcctgtggt tcgtgggatt gcaaagtcca acgccaccgt catcattcgg cagaatggct
     2401 acgtgattta tcagtctgcc gttccacaag gtgcttttga gatcaccgat ctcaacaccg
     2461 ccagtacagg tggcgatttg gacgtaacca tcaaagaaga agacggtagc gaacaacgat
     2521 tcacccaacc ttatgcttca ttggcgattc ttaaacgtga aggtcagaca gatgttgatg
     2581 ttagcgtggg tgaattgcgc gatgaagacg gatttacacc ggacgtcctt caggcgcaaa
     2641 tacttcatgg tttttcccac gggatcactt tatatggagg tatgcaggct gctgaaaatt
     2701 atggttctgc agctctgggt gtcggtaaag atcttggcgc tttgggcgca atttctttcg
     2761 atgtgacaca tgctcgtgcg aattttagcc atgatgatac agaaacgggt cagtcatatc
     2821 gctttctcta ttcaaaacga tttgacgaca cagacactag cttgcgcctg gttggctatc
     2881 gttactccac cgagggctac tataccctca atgagtgggc atcgcggcgc aacagccctg
     2941 aagacttttg ggaaacaggt aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt
     3001 tggggagaga ttatggcaat ttatacctga cattaagccg gcaacaatac tggcataccg
     3061 atgatgtcga acgattaatg caatttggct acagcagtag ctggaagcgt ctctcgtgga
     3121 acgtctcctg gagttattcc aatactgcca gacaggggac ggggaacaac catgccagtg
     3181 ataacaccag tgagcagatc tacatgctct ctttatctgt tcctttatcg ggctggtggg
     3241 gtaatagtta cgccacctat tctgtttcgc aaaacgataa ttccggtagc tcacatcaac
     3301 tcggactcag cggtacggcg ctggaaagaa ataacctttc atggaattta atgcagtcct
     3361 ataacagtca tgatgatgag gttggcggta atatgtccct gacctatgat ggctcttatg
     3421 gcacggtgaa cggcagctat aactacagcc aaaattccca gaggctgaat tatggtatca
     3481 gagggggaat tctggcacac agcgaagggg taacgttaag tcaggagtta ggtgaaacta
     3541 ttgctcttgt taaagcacct ggggccgccg ggttagaaat agataatatg cgcggtgctg
     3601 cgacggactg gcgcggctat acggtcaaga cacagctaaa cccttatgat gaaaatcggg
     3661 tagcaatcag cgataactat ttctcgaagt cgaatataga acttgataat accgtcgtta
     3721 cgatggttcc cacgcgtggt gcagtggtta aagcggagtt tgtgactcat gtgggttatc
     3781 gcgttctctt cagagtgtta aatgcaaatg gtaaaccggt accttttgga gccattgctg
     3841 cgatacaaga tgcaagtttg gcagattcag gaattgtcgg tgaccgtggc gaactttatc
     3901 tttctggtct accagaaaaa ggacaggtta cgttatcctg gggagaaaac gcctcaacaa
     3961 aatgcatctt caattattca ctttcgacac cagaaagtga gagcggatta attgaacagg
     4021 gtgtgacatg tcattaaaag gatttttatg aacaagtata tcaaacagtg gtgctttgct
     4081 gtgtttatgc tctcgttaag tagtgtagca cttgcagctc ctaaaggtat ctgtacccct
     4141 gataatggcg tatttcacag tacgcttgat ttctcgggat atcttattac cgcgaacgaa
     4201 aataaggttg gaacgacatt taacaccact gtaactaatg gatcctcata tcctggacgc
     4261 tgtcattgtg atactggtaa cgtaggagaa ttcccctata tctattatac gtcaaaaata
     4321 aaccaggcat taacttatgc tggggttcac tctaatatta attattatga tcttaatccg
     4381 aaccttgatg ttgggattgc tatagatatt cttggggttg ggtatgtgaa tgcacctttt
     4441 gaatatcatg ctaacaaccc gtctggtaat acaaagtaca attgcaaccg cattgaacct
     4501 ttaagtatat ctagtggggc taaagcgata gtatattttt atatcaagaa gacatttgca
     4561 ggaaaattga ttattcctga aacaaagata gtgacattgt atggaacaat tagccgtgac
     4621 accccggtgg attactcaca gccgatggct gatgtttata ttcggggcga tattacagct
     4681 ccgcaaagtt gcgaaataaa taatttacag ccagtttatt ttgattttaa agaaatacct
     4741 gctgcagatt tttcatctgt tgttggaagt gcggtaacaa cacataaaat tactaaaacg
     4801 gtcactattg agtgtgaaaa cctggggata ctgaataccg atgatatcag tacgtctttt
     4861 tatgctaccg aacccaatac ggacaattca atggtcgtaa cttcaaactc taacgtgggg
     4921 ataaaaattt acgacaaaaa taataaggaa atcaaagtga acggtggtga gttgccaaca
     4981 gacatgggta aatcaacagt gtatggtgaa aaatcaggta gtgtaacttt ttcagctgct
     5041 cctgcgagcc ttactggagc tcgcccagcc ccggggcaat ttactgcaac agcgacgata
     5101 actgttgaga ttgtgcgcta ataggtctga caacgattgt tcatcacatc gttgtcatct
     5161 cacccatttc tttatcactt ttaaatgcat catgacaaaa agatgacatg tgggattttc
     5221 atcttcacat ttaaaatcag taagttattt ggtaattcct ttctgtcata aaaatgtcat
     5281 attccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc caacaaacaa
     5341 tgctttatga atcctcccag gagacattat gaaagttatg cgtaccaccg tcgcaactgt
     5401 tgtcgccgcg accttatcga tgagcgcttt ctctgtgttt gcagaagcaa gcctgacagg
     5461 tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt accagaaaga
     5521 aaccggtaat aaagttaact accagggtat cggttcttcc ggtggcgtaa aacagattat
     5581 cgctaatacc gttgattttg gtgcctctga cgcgccgctg tctgacgaaa aactggctca
     5641 ggaaggtctg ttccagttcc cgaccgtgat tggcggcgtg gtgctggcgg ttaacattcc
     5701 agggctgaag tctggcgaac tggtgctgga tggtaaaacc ctcggcgaca tctacctggg
     5761 caaaatcaag aagtgggatg atgaagccat cgccaaactg aatccgggtc tgaaactgcc
     5821 ttcacaaaac attgctgtag tacgccgcgc agatggctcc gggacttcct tcgtcttcac
     5881 cagctacctg gcgaaagtga acgaagagtg gaaaaacaac gttggtactg gctctaccgt
     5941 aaaatggccg atcggtctgg gcggtaaagg taacgacggt atcgccgcgt tcgttcagcg
     6001 tctgccgggt gcaattggtt atgttgaata tgcttacgcg aagcagaaca acctggcgta
     6061 caccaaactg atctccgctg atggtaaacc ggttagtccg accgaagaaa acttcgctaa
     6121 tgcagcaaaa ggtgcagact ggagcaaaac cttcgctcag gatctgacca accagaaagg
     6181 cgaagatgca tggcctatta cctctaccac gttcattctg atccacaaag atcagaagaa
     6241 accagaacaa ggcacagaag tgctgaaatt cttcgactgg gcgtacaaaa ccggggctaa
     6301 acaggcgaac gacctggatt acgccagcct gccggatagt gtagttgaac aggttcgcgc
     6361 tgcgtggaag accaatatta aagacagtag cggtaagccg ctgtactaat aaaactccag
     6421 gccgggtacg gtgttttacg ccgcatccgg cattacaaaa tgactttgta aacgcgttta
     6481 actgaagagt aacttatggc tgcaaccaag cctgctttta acccaccggg taaaaagggc
     6541 gacataattt tcagcgtgct ggtaaaactg gcggcgctga ttgtgctatt gatgttgggt
     6601 ggcattattg tctctctgat catctcctcc tggccgagca ttcagaaatt tggtctggct
     6661 ttcctatgga ccaaagagtg ggatgcaccg aacgatatct acggggcgct ggtgccgatc
     6721 tacggtacgt tggtgacttc gtttatcgcg ctgctgatcg ccgtcccggt gagtttcggt
     6781 atcgccctgt tcctgactga gcttgcgcct ggctggctga aacgcccgct gggtatcgcc
     6841 attgagctgc tggcagccat tccaagtatc gtttacggca tgtggggcct gtttatcttt
     6901 gcgccgctgt tcgccgttta ctttcaggag ccggtcggca atatcatgtc gaatatcccg
     6961 attgttggcg cgctgttctc tggccccgca tttggtatcg gtatcctcgc ggcaggcgtg
     7021 atcctcgcca tcatgattat tccgtacatt gcggcggtaa tgcgtgatgt gttcgaacaa
     7081 accccggtga tgatgaaaga gtcggcctac ggtattggct gcaccacctg ggaagttatc
     7141 tggcgtatcg ttcttccgtt caccaaaaat ggtgttatcg gcggcatcat gctggggctg
     7201 ggccgcgcgc tcggtgaaac catggcggtg acctttatca tcggtaacac ctaccagctc
     7261 gacagcgcct cgctgtatat gccgggcaac agtatcacct ctgcgctggc gaacgaattt
     7321 gcggaagcgg aatccggtct gcacgttgcc gcactgatgg aactgggcct gatcctgttt
     7381 gtgattacct tcatcgtcct cgccgcatcg aagtttatga ttatgcgcct ggctaagaat
     7441 gagggggcac gctaatggct atggttgaaa tgcaaaccac tgcggcgctg gctgaatctc
     7501 gccgcaaaat gcaggcgcgt cgccgcctca aaaaccgtat tgcgctgacg ctctcgatgg
     7561 cgacgatggc cttcggcctg ttctggctga tctggatttt aatgtccacc atcactcgcg
     7621 gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac gccgccgccc aatacggaag
     7681 gtggtggtct ggctaacgcc ctggcgggta gcgggctgtt aattttgtgg gccacggtat
     7741 tcggtacgcc gctgggcatt atggcgggga tttatctggc ggaatatggt cgtaaatcct
     7801 ggctggcaga agtgattcgc ttcattaacg acattctgct ctccgcgccg tcgattgtgg
     7861 ttggtctgtt tgtctacacc atcgtggtgg cacagatgga gcacttctcc ggctgggcgg
     7921 gcgtaattgc gctggcgctg ttgcaggtgc cgatcgttat ccgcaccacc gagaacatgc
     7981 tgaaactggt gccggacagc ctgcgtgaag cggcttatgc gctgggtaca ccgaagtgga
     8041 agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg gattatgacc ggtatcctgc
     8101 tggcgattgc ccgtattgcc ggtgaaaccg cgccgctgct gtttaccgcg ctctccaacc
     8161 agttctggag cacggacatg atgcagccga tcgccaacct gccggtgacg atctttaagt
     8221 ttgcgatgag cccgtttgcg gaatggcaac aactggcctg ggccggggta ttgatcatta
     8281 ccctgtgcgt cctgctgctg aacattctgg cgcgcgttgt ttttgcgaag aataaacacg
     8341 gttaacactt tgccggatgc ggcgtgaacg ccttacccgg cctacggtaa gcctgattag
     8401 cgaaaagcat caggtacgat gaggaaaaga ttgcaatgag tatggttgaa actgccccga
     8461 gtaaaattca ggttcgtaat ttgaacttct actacggcaa attccatgcc ctgaaaaaca
     8521 tcaacctgga tatcgctaaa aaccaggtaa cggcgtttat cgggccgtcc ggctgcggta
     8581 aatcgacgct gctgcgtacc ttcaacaaaa tgtttgaact gtacccggag cagcgtgcgg
     8641 aaggtgaaat tctgcttgat ggcgacaaca tcctgaccaa ctctcaggat atcgccctgc
     8701 tgcgtgcgaa agtgggcatg gtgttccaga aaccgacgcc gtttccgatg tccatctacg
     8761 acaacatcgc ttttggcgtt cgtctgtttg agaagctctc ccgtgccgac atggacgagc
     8821 gcgtgcagtg ggcattgacc aaagccgcat tgtggaacga aaccaaagat aaattgcacc
     8881 agagcggtta ctctctctct ggtggtcagc aacagcgtct gtgtattgcg cgtggtatcg
     8941 ccattcgccc ggaagtgctg ctgctcgacg aaccgtgttc ggcgctcgac cctatctcta
     9001 ccgggcgtat tgaagagctg atcaccgaac tgaagcagga ttacaccgtg gtgatcgtca
     9061 cccacaacat gcagcaggct gcgcgttgtt ccgaccacac ggcgtttatg tacctgggcg
     9121 aattgattga gttcagcaac acggacgatc tgttcaccaa gccagcgaag aaacaaacag
     9181 aagactacat caccggtcgt tacggttgat tcaggagtgc gttatggaca gtctcaatct
     9241 taataaacat atttccggcc agttcaacgc cgaactggaa agtatccgca cgcaggtgat
     9301 gaccatgggc ggcatggtgg agcagcagct ttctgatgca atcaccgcga tgcataacca
     9361 ggacagcgat ctggcgaagc gcgtgatcga aggcgacaag aacgtcaaca tgatggaagt
     9421 ggcgatcgat gaagcctgcg tgcgcattat cgccaaacgt cagccgacgg cgagcgacct
     9481 gcgactggtt atggtgatca gtaaaaccat tgccgagctg gagcgtattg gcgacgtggc
     9541 ggacaaaatc tgccgtactg cgctggagaa attctcccag cagcatcagc cgttgctggt
     9601 aagtctggag tcgctgggcc gtcataccat ccagatgctg cacgacgtgc tggacgcgtt
     9661 cgcgcggatg gacattgacg aagcggtacg tatttatcgt gaagataaaa aagtcgatca
     9721 ggaatacgaa ggtattgttc gtcaactgat gacctacatg atggaagatt cgcgtaccat
     9781 tccgagcgta cttactgcgc tgttctgcgc gcgttctatc gaacgtattg gcgaccgctg
     9841 ccagaatatt tgtgagttta tcttctacta cgtgaagggg caggatttcc gtcatgtagg
     9901 cggtgatgag ctggataaac tgctggcggg aaaagatagc gacaaataat tcaccagaca
     9961 aatcccaata acttaa
//
LOCUS       DAZFFI010000153         9904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFFI010000153 DAZFFI010000000
VERSION     DAZFFI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.153"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..145)
                     /locus_tag="ACHJ12_004432"
     CDS             complement(<1..145)
                     /locus_tag="ACHJ12_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992127.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFS"
     gene            223..1317
                     /gene="ddlA"
                     /locus_tag="ACHJ12_004433"
     CDS             223..1317
                     /gene="ddlA"
                     /locus_tag="ACHJ12_004433"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4992128.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1341..1553)
                     /gene="yaiZ"
                     /locus_tag="ACHJ12_004434"
     CDS             complement(1341..1553)
                     /gene="yaiZ"
                     /locus_tag="ACHJ12_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4992129.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1813..2121
                     /gene="yaiY"
                     /locus_tag="ACHJ12_004435"
     CDS             1813..2121
                     /gene="yaiY"
                     /locus_tag="ACHJ12_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4992130.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2180..3274)
                     /gene="yaiW"
                     /locus_tag="ACHJ12_004436"
     CDS             complement(2180..3274)
                     /gene="yaiW"
                     /locus_tag="ACHJ12_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4992131.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASSVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3287..4507)
                     /gene="sbmA"
                     /locus_tag="ACHJ12_004437"
     CDS             complement(3287..4507)
                     /gene="sbmA"
                     /locus_tag="ACHJ12_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4992132.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4859..6016
                     /gene="ampH"
                     /locus_tag="ACHJ12_004438"
     CDS             4859..6016
                     /gene="ampH"
                     /locus_tag="ACHJ12_004438"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4992133.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6017..6640)
                     /gene="iprA"
                     /locus_tag="ACHJ12_004439"
     CDS             complement(6017..6640)
                     /gene="iprA"
                     /locus_tag="ACHJ12_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4992134.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6728..9616)
                     /locus_tag="ACHJ12_004440"
     CDS             complement(6728..9616)
                     /locus_tag="ACHJ12_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4992135.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYNTFGYHDDAVTLFN
                     WGDNTKTDHDYLTYGGYVYDHAADGYFDTVFSGDTVNGVISTYYLNHDYGTDTANTLN
                     ITNSNIHGMITSDQIGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFN
                     DTYLDADGKTSKTDYDRLVTAALGTAVTLDVESNINISNNSHVAGITLVQNDLGNATY
                     NTEGHQWDNNIVVNNSTVTSGSLSEDEQSDRGHFGNSVEPSDYGNGASGADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKDSLIDTLAINSGSQVNVSADSSLTSDTITLNGSSSMEV
                     NGEVNTDHLIIDTFSTVNFGEDTASAWTSAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSVDNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDRSANVGVKYTW"
BASE COUNT         2592 a         2428 c         2341 g         2543 t
ORIGIN      
        1 gagaaaaaaa ctcaccgcat ggccatttaa gccaggaata aattagcttc ccgttcatca
       61 tttttgtgat cttgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg
      121 cttcgtcagc agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt
      181 ggtaatgtat gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta
      241 ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac
      301 attgtcgatg ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa
      361 gggcaatggc acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat
      421 attgcgttgc gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag
      481 cttatcgacg cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc
      541 cacggtacgc tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg
      601 tttgtaggtt ctgatgttct ggcttcagca gcctgtatgg ataaagatgt caccaaacgt
      661 ctgctacgcg atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt
      721 cacaacatca gttttgccga agtggagtct aaactggggt taccactgtt tgtaaaaccg
      781 gctaatcagg gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt
      841 gccgtcgatc tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt
      901 cgtgagatcg aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag
      961 atcgtactca ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg
     1021 aaagtggtag ttccggcagc cattgcacca gagatcaacg ataagatccg ggcgattgcc
     1081 gttcaggctt atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc
     1141 ccagagaacg aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt
     1201 atgtatccga agctgtggca agccagcggt ctcggttaca ccgatctgat cacacgtttg
     1261 attgagctgg cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat
     1321 tttaacgccc gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata
     1381 atcaaccccg ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc
     1441 caaccttttg cttcaaatcc cagtaacccc accacgccat taagaatgaa tataaggcca
     1501 atggcgaacg catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct
     1561 ccgttaacga aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac
     1621 attttcgtaa ttgttaataa atttaactta aggttgccat tttgtgactc acaacgaaga
     1681 ttcacgcttc gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa
     1741 atgacctgtc agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt
     1801 ccggaggttt ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc
     1861 aatgcctctt caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg
     1921 gctggggcgc aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg
     1981 caggtccagg aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc
     2041 gggctgatcc cgtttttagt aggctgcctc atttttgcag tggtggcgct atggctgcac
     2101 tggcgacatc gccgccagta agatttacgt tgccagatac ggcctaaaac gccttatctg
     2161 gcatgacatt cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct
     2221 tcgcaaacca ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag
     2281 gcaacatttc gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct
     2341 tgtacagcgc cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt
     2401 cgctgtcgct catccccagt tttgccgcca gtttgcgcgt tgccagttcg gttttcccgg
     2461 gttctttact gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ctgctggcct
     2521 tactgactgc gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag
     2581 caaagcggta tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagtaccaa
     2641 accacaaccc gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg
     2701 gatacccttt ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc
     2761 gcacaggatt aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt
     2821 caaaaatcgc gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt
     2881 tgccgtttgg cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc
     2941 gttcagcacg acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat
     3001 ctgcctgata atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg
     3061 tcggtgccag cccctgactt tcaaaggtgg tggcgagatc tttcgcccag gcatcgcggt
     3121 ctttcacgct cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc
     3181 cctttttcaa cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca
     3241 atgacagcga agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat
     3301 gggttacttc ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt
     3361 agatagacat caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc
     3421 gaacctgacc aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa
     3481 caattgacgg aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc
     3541 gggcgatgtt gaaatacata tagtgaaaat agaggcggaa atagtttttc cgcacggcgc
     3601 taaacagctc gcgtaccgta ggcggcgtcg cacgcgtggc atcgtcttca ccataaacca
     3661 gctctttacg gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga
     3721 tccctaccac tgccagcaat ccggtcccca tcagcgacca gacgatagcg gcaatcacca
     3781 gaccatacgg aatgtgcccg ataatcggca gctccggcac atgcgtggag agcgttacca
     3841 gcaccggcag gaaggcgatc aacgtcatga tggcgttgat aaaactgacg cccatattct
     3901 ccagcgttga agcaaaacgc atggtgtctt cctgcacacg ctgtgcggcc ccttcgatat
     3961 gacgcagttg ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca
     4021 cgtagtgact gacaaagaag ttgttcagca cactgatcac cacagcgatc agcgcaatcc
     4081 ccagaaagac gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta
     4141 gcgcggtttg aatcagatca tagaacggtg cataccaggc gttgacggcg accccgactt
     4201 ccaccaaaaa ccaggtgacg aagatgatca gtgcagtacc gagtattgac cagtattgcc
     4261 aacggtgcgg gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt
     4321 aagcgtaaaa aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc
     4381 cggaagcgcc ggtgatacgc gccacccagt cacccccacc ggcttgccag aagataacgg
     4441 caatcaatgc ccaaacaaag gccgagagaa aaaacgctcc cggctttggg aaaaaagact
     4501 taaacatatg cactcctgct aacttcttat cgttttaaca actgctgtgt tttaaccgca
     4561 tgaccgctac aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt
     4621 agttcgccag cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta
     4681 cataagcgga ttaacacgcg caggatcaca aatgcaggca ttggtgagca ggaaaaagat
     4741 cgtgtttacg cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc
     4801 catcaatgag tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt
     4861 gaaacgtagt ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc
     4921 ggcgcagccg atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca
     4981 tattttttac ggcagcggtg ccacggggat ggcgctggtt gttatcgacg gtaaccagcg
     5041 cgtctttcgt agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc
     5101 ggtcgtgcgt attgcttccc tcaccaaact aatgaccagt gaaatgctgg tgaaattgct
     5161 cgaccagggg accgtaaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg
     5221 cgtgccgacc tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc
     5281 cctgccccgt gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg
     5341 cgaacaacgc tggaaatacc tttctacggc gaagctaaaa gccgcgccag gcagtcaggc
     5401 ggcgtactct aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa
     5461 gccttatacc cagttgtttg aagagcagat tacccgtcca ttggggatga aagacaccac
     5521 ctacaccccc tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc
     5581 gtgcaataac acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat
     5641 gatgcgctgg atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga
     5701 tcgcatgcag acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt
     5761 ccccggcaaa gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg
     5821 tccggggatt attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat
     5881 cccgcagaag aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa
     5941 aaatatgagc gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt
     6001 tatccccgca tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt
     6061 tattcatttc gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac
     6121 gcgatatgcg tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt
     6181 cattccagtc aatcattgat aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa
     6241 tgagctgcac gtcgcgtaat tccagaattc tattttgcca ggctaaccag taaaaagcgt
     6301 ctcgccagag ttgattttgt tcaataagcg taatggtttg tttggctggt agatgatatc
     6361 ccgtacaatt tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag
     6421 ccagccccat aatataagga gcctgggtaa taccgataag tacgttttct tctctacgca
     6481 gagagataac gccctctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata
     6541 taacttgctt ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga
     6601 gcttaccaaa ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa
     6661 tgcccgtcaa atccttgacg ggcattattt agattaaatt accagaattt cttcggagtg
     6721 aagaatatta ccaggtatat ttaacaccca cgttcgcgga cctatcttga tctacgtcac
     6781 caccaccgag gtagttagca tcggtatagg cgctgaagtt cttggtgaag ctgaactgag
     6841 tacccagacc aacacgtacc gcagaccctt cagtaccgtt atcgatggaa tcaccgttca
     6901 catcgttatc gttgttagag tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag
     6961 cctggtcttc gctgtaggtg aaggtataac ctgcatctac acccagttca taacgcatgc
     7021 tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag ctggtagtca tcaccagact
     7081 ggaacagacc agaaatgctg ccgtaaggag tcacataacc agcatcaccc agtttgaagt
     7141 cgtaaccggc tttcaagccg aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag
     7201 taccgttgct catggttgca gacaggtcgt tgttgaagtg agagtagctc aagctaccat
     7261 caacaaagac gttgttcgcg aagtgagcag aagagtagat gtaggcagtc tggctgtctt
     7321 gatctacctg accagaacgg tcattcatgt cacctttagc gaagcctgca gccgcaccga
     7381 cgatccactt agcgttgtta ccgtcaattt tggtatcaac accgaccatg atgccgttaa
     7441 catcctgatc atagttgatg gtgccgttgt cgccgttgaa gttaccaccg aagtagctta
     7501 cccatgcgcc gccgttatca gccaggccat gacgagagtt ggtcagacga gtaccaacgg
     7561 tgtcttgttc caggttccag atattggtgt tcgcagaagg gatgctcagc gccatgttag
     7621 cgtagtcggt cagctccatc tgttgcagaa caacggtgtt accgcgctgt tcagcctgat
     7681 aggtgtatgc acccaggtca gctttgttag cagcagagaa ggtcgcgttg gttttggtat
     7741 catcgatata gatgagctgc ttacctgcgt aatcagcaat cgcgccagca ccagtggagt
     7801 tgtcgatacg aactttgtag ttaccggttg cagctacaac gttgtcaaca gagctgttgt
     7861 caacttcgtc gccattatta atgtcgttgt taccattaac ggtcaagtga ccatcagagt
     7921 tcatcgcaat aacaccataa ccatagttag ctttgctggt gtcgttggta cgatcgttga
     7981 cgaggtctgc attcaggatg tagtcattac tatgaatgtc aaacacacct gcgtaaacat
     8041 taccgttgtt atctttaacg ttgccactgg tcagttccag agtatcagtt gcaaatacgc
     8101 tggagatgtc attcatgttg gtgtttacat ccagcacacc accgttagta acggtaatgg
     8161 tgttggcgta cagcggtgca gatgtccaag ctgaagcagt atcttcaccg aagttaacag
     8221 tagagaaggt atcaatgatc aggtgatcag tgttaacttc accgttaact tccattgaag
     8281 aactaccgtt cagagtgatg gtgtcggaag tcagggaaga atctgcactc acgttaacct
     8341 gagaaccgct gttgatagcc agggtatcga tcagagaatc tttcgtggtt tcccaatcag
     8401 aaccattgtt cagaacaaca tcgaatacgc cactctggaa tgcgttagcg ttatcaacac
     8461 gattccagcc atcttcagag taagaagcgt acgggttcag gctgtttggc gcaacgtcgt
     8521 agaaggcttc gtcagcaaca acaacgtcat aatcaatatt cgctgaacca acccatttgg
     8581 tgttgttcag agtcaggttc aggtgatcga cgttctgatc gtcagtagcc caaccgccat
     8641 gagtgtaagc agtggtagag ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc
     8701 aggtactgtc aaatacaacg tcgccaagca actgagaatt gttgaatgta acgttgttga
     8761 ccatacgata atcagaagtg tcatcatcta tgaaggctaa tgcaacatca tcagcacctg
     8821 atgcaccatt accatagtcg cttggctcaa cagagttgcc aaaatgacca cgatctgatt
     8881 gttcgtcttc tgagagcgaa cctgaagtta cagtggaatt attaacaacg atattgttgt
     8941 cccactgatg accttcagta ttgtaagtcg cattacctaa atcgttttga accagagtaa
     9001 tacctgcaac atgggagttg ttgctgatat tgatgttact ttcaacatcc agagttacag
     9061 cggtgccaag agcagctgtt accagacgat cataatctgt tttagacgtt ttaccgtctg
     9121 catccagata agtatcattg aagtagaatg catcaaaatc atcgtcaatt gttgagttcg
     9181 cgatgttcag agtgaaaata tcgccatcta cccaatcatg accagtataa tcgctaccgt
     9241 tggtccagac gtaatcaccg tatccgatct gatcggaagt aatcataccg tgaatatttg
     9301 agttcgtaat attcagagta ttagcggtat ctgttccata gtcgtgattc agataataag
     9361 tagaaattac accattaacg gtatcgccac taaatacagt atcaaaataa ccatcagccg
     9421 catggtcgta tacatagcca ccatacgtca aatagtcgtg atcagtttta gtattgtcgc
     9481 cccaattgaa tagagtgact gcgtcatcgt gatacccgaa tgtattataa gtagttgcat
     9541 tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct
     9601 ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta
     9661 tcaatgcata gaaaacatat tatgtttcgc gctaatgatt atgctgcgtt acagacgaat
     9721 ggttcaatta ttctttcccc taagtccgaa tgtggacata tataacctat ggagaaaaac
     9781 acgcatcaaa ttgataaaag ggcgatattt caacatgttg cagataatgt tatttttgat
     9841 acatacgcgt tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca
     9901 aagt
//
LOCUS       DAZFFI010000154         9865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFFI010000154 DAZFFI010000000
VERSION     DAZFFI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.154"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..91
                     /locus_tag="ACHJ12_004441"
     tRNA            16..91
                     /locus_tag="ACHJ12_004441"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Thr,seq:ggt)
     gene            131..246
                     /gene="rrf"
                     /locus_tag="ACHJ12_004442"
     rRNA            131..246
                     /gene="rrf"
                     /locus_tag="ACHJ12_004442"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(309..1067)
                     /gene="yhdZ"
                     /locus_tag="ACHJ12_004443"
     CDS             complement(309..1067)
                     /gene="yhdZ"
                     /locus_tag="ACHJ12_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4992136.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1075..2178)
                     /gene="yhdY"
                     /locus_tag="ACHJ12_004444"
     CDS             complement(1075..2178)
                     /gene="yhdY"
                     /locus_tag="ACHJ12_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4992137.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2188..3369)
                     /gene="yhdX"
                     /locus_tag="ACHJ12_004445"
     CDS             complement(2188..3369)
                     /gene="yhdX"
                     /locus_tag="ACHJ12_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4992138.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3437..4462)
                     /locus_tag="ACHJ12_004446"
     CDS             complement(3437..4462)
                     /locus_tag="ACHJ12_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4992139.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4893..5114)
                     /locus_tag="ACHJ12_004447"
     CDS             complement(4893..5114)
                     /locus_tag="ACHJ12_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992140.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5367..8471)
                     /gene="acrF"
                     /locus_tag="ACHJ12_004448"
     CDS             complement(5367..8471)
                     /gene="acrF"
                     /locus_tag="ACHJ12_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO4992141.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8483..9640)
                     /gene="acrE"
                     /locus_tag="ACHJ12_004449"
     CDS             complement(8483..9640)
                     /gene="acrE"
                     /locus_tag="ACHJ12_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4992142.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2621 a         2553 c         2368 g         2323 t
ORIGIN      
        1 catcaaattt agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag
       61 gtccccagtt cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga
      121 aaagaatttg tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
      181 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
      241 ccagacatca aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta
      301 acattcaatt aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc
      361 gcaaaaaatt catcaggtgc agcttgttcc actatctccc cacgatccat aaaaattacc
      421 cggtcagcga cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc
      481 gactgcgcca gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc
      541 gacgttggct catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca
      601 acgcgttgct gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt
      661 ctcacccgct caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt
      721 acccaaatcg gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa
      781 tgctgaaaga ccattcccac ttcctgccgg acgcgctcaa tattgcggat atcttcatta
      841 agttcgatgc catctaccgc gatccgtccc tgttgatgtt cttccagatg attaatacaa
      901 cgaatggttg tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt
      961 tgcacggtta aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt
     1021 tccagcgtaa tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg
     1081 cggtgtacgc ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa
     1141 acagaagatc cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca
     1201 ggtgggatca acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat
     1261 caccaggctg gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag
     1321 cttcaacgcc tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga
     1381 ctctgccgct tcatactgcc ctttaggcag tgcctgtaat ccgcctcgca cgacttccgc
     1441 aacatatgct gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact
     1501 ggtgccttct gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag
     1561 cggtacgccg cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg
     1621 ggagcggcga cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga
     1681 tgcaataatt aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc
     1741 aaaaaaaccg ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc
     1801 aatatagcga ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg acacaagccc
     1861 aatcagtaat gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa
     1921 ctgaccaaat cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg
     1981 cgttgagcca acccagttag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc
     2041 ccacatcaac catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt
     2101 ttttcgcacc cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga
     2161 cagcaatact tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata
     2221 gatattcatc agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc
     2281 tatcgtctcg atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat
     2341 atcgggatag ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct
     2401 ggttaacggc ggaataatca ctcgcagtgc ctggggaata atgacctggc gtagcgtaac
     2461 cgggttgggt aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat
     2521 cccagcgcgg ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa
     2581 ggctgccagt tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg
     2641 gacatcccag tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat
     2701 caacactgcg gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt
     2761 attaaagcgg aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc
     2821 aataaatccg tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc
     2881 cagatcaaac gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta
     2941 ccagaagaag atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga
     3001 aagctttcgt agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac
     3061 agaagcgaac acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa
     3121 aacgcgtccg taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc
     3181 agcgccgcga tccagaaagg caaaacctga agtaatgcca cgattactga gattcgttac
     3241 agtgttgtga aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa
     3301 taaccaggca cgaaccgtag ggttggcaaa ggagagtgag cctttaacgg ttgagcggcg
     3361 atgagacata gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc
     3421 gttacctcac accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga
     3481 gattattttg cccacgttta attttcagcg ggctttctga acctacatta cgctcgaaaa
     3541 tttccgagta gttacccacc tgtttgatga tgttataggc ccatttatta tccaacttca
     3601 gatccttgcc gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat
     3661 tagccgcttt ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg
     3721 cgaaaagcgt ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc
     3781 caagcggttc tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt
     3841 tgatgcgcag ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt
     3901 ccagtgcctt cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt
     3961 ttgccttgaa atagtcggca acgttgagtt cggtatcagt acccgcctga atacagacgg
     4021 tagcgccatc cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa
     4081 tgccgtcgta ataagtgacg ccagtaaatg ccattcccat cccggcgtcg cgagatgaag
     4141 tccaggtcgt attacgggag agcaaatcca cttcccctga ctgtaaagcc gtgaagcgtt
     4201 cttttgctgt gagtggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa
     4261 caccacgaca aacatcaaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga
     4321 acccaggtaa tccatcactg atcccgcatt gcacaaaacc ttttttctga acggcatcaa
     4381 gcgtcgcgcc agcatgcgcc tgatttgcaa cggcaagcag cacgctagcg gcagccagtg
     4441 tggctatcat catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg
     4501 tttgggaaac gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccagc
     4561 ttttgcgccc gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc
     4621 gccccgcaat ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt
     4681 gttttactaa gcagaataat gattgttagc gtgagtatat ttctggtggg aagtgtgaaa
     4741 gttaaattga tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaatcag
     4801 cagagaatta gccagcagaa tatagcaata ttttatctta tgtaaagata ctgtatcgtt
     4861 aaacagagcg cgaaactccg cgccctgttc agttatttcg acagagcgat gatccccatc
     4921 gtcaggccag caacagccgc tgccccgccg gcaatagccc cagccgtttc ccagttatcc
     4981 tgagtcgtgc ctttcataca ggtattggtg tgaaccagac gaccctgatc gtcataaacc
     5041 ggaacgcagg gcgaatcgtg agcacagccc gccagcaatg cggtgagcaa tgccacagga
     5101 attaatcttt tcatggtgtt acctcagtaa taaacgctaa cgcttaataa gttaactctc
     5161 ttattaacga gctgaatgcc attttatcac cggaggttat aaatattgcg gaatgaataa
     5221 tgacaaatta tgtgaattgt aaaaatagtg aagggagcgg gaactataga aagtttttaa
     5281 agagtgagtg gaagaatatc ttattaattt ttatgggttt cactgaaaat aataaaggca
     5341 cccgaaagtg cctttatgtt tctgatttat cctttaaagc aacggcggat caccacaaag
     5401 aacaccggta cgaagaagat tgccagcaac gttgcagaga ccattccgcc cattaccccg
     5461 atacccactg cgttctgcgc gccactgccg gcaccgttac tgatagctag cggtaatacg
     5521 ccgagaataa aggcgagaga ggtcatcagg ataggacgca gacgcatacg tactgccatt
     5581 agtgtcgctt caacaacacc tttaccctct ttctccatga gatctttagc gaactcaaca
     5641 atcaaaatag cgtttttggc cgacaagcca attgtcgtta gcaagcccac cataaagtag
     5701 acgtcatttt tttgattaaa gagtgtcgcc gccagcagca cgccgacaat ccctaacggc
     5761 acgaccaaca taaccgagac aggaattgac cagctttcat agagtgcagc aaggcacagg
     5821 aaaacaacca caaaggaaat tgctaccaga gcgggagcct ggtttcccga taagcgttcc
     5881 tgatacgaca tacccgtcca gtcataacca atgccagcag gtaattttga cgcaaggttt
     5941 tccatcaacg ccatggcatc gccggaactg gttcctggcg cggcttcccc ctgaatctcc
     6001 attgacggca gaccgttgta gcgttccagt cgcggagagc catacaccca atgtgaagtg
     6061 gtaaaggccg agaatggcac catttcgccg ttggcgctgc ggacataaag tttatcgaca
     6121 tcttctggca gcatacggaa tttggcatcc gcctgaacat acaccttttt cacgcgacca
     6181 cggtcgatga agtcgttaac gtaagtccca cccaacgccg ttgaaatggt ctgattgatg
     6241 tcagaaagtg aaacacctaa tgcctgcgcc ttttcctggt caacttccag tttaaactgc
     6301 gcggtgtctt ccaggccatt aggacgcacg ctgactaagc tggcaggatg ttgcgccgcc
     6361 ataccaagca actggttacg ggcctgggtt agggcatcgt gacccagccc agcctgatca
     6421 attaactcaa agtcgaaacc cgttgccgtg cccagttcaa caatggctgg catattgaat
     6481 ggaatgacaa aaccgtcgcg gatcttgcca aattccattt tggcacgatg gattaccgct
     6541 tccgcactgt tttcatcacc attacgctct tcccacggtt tcagactgac gaaggccata
     6601 ccggcgtttt gtgcctggcc gctgaagcta aagccgttaa ccgtaaagac actttcaacg
     6661 ttcgctttct cgttcttcag atagtaatcc gtcacttgat ccaacacttt ttgcgtccgc
     6721 tcttgcgtcg cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca
     6781 ggtaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac aatcagcgca
     6841 tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt ggtgtagtgg
     6901 ttaacgctat gatcgaaggt agtattaaac cagccgaaga aaccgccctt attttcgtga
     6961 tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag aatcaatgcc
     7021 accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg ataaattgcc
     7081 ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac catcgcgata
     7141 cccaccagtg cgccctggat ttgcgacatt gatttttccg tcgcttcttt tggcgggagc
     7201 ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc atcgacgagc
     7261 agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta accaaaagcg
     7321 gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg gatcagcgtt
     7381 gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat ggcttcgaac
     7441 agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc ataagggtag
     7501 agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc cttaatggct
     7561 ttcgcggtat cgagagcatt cgcgccggtt gccagcttaa tccccaggcc cgccgccggt
     7621 tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac ccgtgcgaca
     7681 tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggtcacttt gccgaattct
     7741 tccggatttt ttaaccgcgt ttgagcaata atcgaggcat tcaactgttg ccctggtaac
     7801 gctggcgttc cgcccaactg tccggcagcg atctgatcgt tctgtacctt caactggtta
     7861 atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag ccagatacgc
     7921 atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg gctaagcgta
     7981 tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc tgggttatca
     8041 gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact gatcccctgc
     8101 tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg cacctgcact
     8161 tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac gctaccggcg
     8221 gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt ctgttcgata
     8281 acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt tgccgaaaca
     8341 gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag gatcgccagt
     8401 gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg tcgaataaaa
     8461 aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta tcggtagtgg
     8521 ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact tgatcgccag
     8581 atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca acgacagggc
     8641 gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca cgcggtgtgc
     8701 ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca tcaatccgtg
     8761 cgcgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagacg gcacgtaggg
     8821 ttatggagcc agtgctttca tcaacggtca catcagagaa ttgcagcgta cctttcaggg
     8881 gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt tccttatgca
     8941 aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat tgggtcacat
     9001 caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc ccattagtga
     9061 caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc gcagtcactt
     9121 tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca atcacggcgg
     9181 catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg atgtatttcg
     9241 tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg gcggcttcac
     9301 ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg ggatcgatct
     9361 ggtacaggga ctggcctgct tgcacatcgc tgccttcagt gaaattgcga ttcagtacga
     9421 tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg cctggtaatt
     9481 cagtcttaac ttctaacggg gccgttttta caatatgaac ggtaacctgc ggttcaccga
     9541 cgtgagcttt ctcttctccc ttatcgttac aaccggcgat taaagccgcg gagatcagaa
     9601 taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct caaaaaacca
     9661 aaagcgcgtt atttacccaa aaaggcaacg cgttaactcg cagaaagaaa aatacagttc
     9721 gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaataca ggcgtctcaa
     9781 ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc ggattgaaag
     9841 aggcggaatt aaaacatcgt aaatt
//
LOCUS       DAZFFI010000155         9530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFFI010000155 DAZFFI010000000
VERSION     DAZFFI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.155"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..931)
                     /gene="rclR"
                     /locus_tag="ACHJ12_004450"
     CDS             complement(77..931)
                     /gene="rclR"
                     /locus_tag="ACHJ12_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO4992143.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1157..2482
                     /gene="rclA"
                     /locus_tag="ACHJ12_004451"
     CDS             1157..2482
                     /gene="rclA"
                     /locus_tag="ACHJ12_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO4992144.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            2591..2827
                     /gene="rclB"
                     /locus_tag="ACHJ12_004452"
     CDS             2591..2827
                     /gene="rclB"
                     /locus_tag="ACHJ12_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO4992145.1"
                     /translation="MFKKSVLIATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2839..3432
                     /gene="rclC"
                     /locus_tag="ACHJ12_004453"
     CDS             2839..3432
                     /gene="rclC"
                     /locus_tag="ACHJ12_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO4992146.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(3591..3719)
                     /locus_tag="ACHJ12_004454"
                     /pseudo
     CDS             complement(3591..3719)
                     /locus_tag="ACHJ12_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            4022..4879
                     /locus_tag="ACHJ12_004455"
     CDS             4022..4879
                     /locus_tag="ACHJ12_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992147.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKPTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(5000..9253)
                     /gene="fdeC"
                     /locus_tag="ACHJ12_004456"
     CDS             complement(5000..9253)
                     /gene="fdeC"
                     /locus_tag="ACHJ12_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO4992148.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAANTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAAIYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         2439 a         2345 c         2160 g         2586 t
ORIGIN      
        1 tggaaataga aatagttgaa aggctcacaa ctcccggaat gcggattatc tttgccctga
       61 tttctggcgg ttttattcag ggtgcaagct gtctgaccct ttcccgatat tctcccggag
      121 tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga ttcactggca tagcctactg
      181 actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc gctatttgta
      241 gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg aaaagctggg
      301 caaaacttgc ccgagacatg tgggcgatgc tggccagcga ttcgacggtc caggcgtgtc
      361 ctggcatttc cagcatttgc tgtattaccg caccaagacg tggatgcaga agcaaactga
      421 gaatgttttt ctctgtatta acctgtgcaa tccactcacg caccgcgagg gtaaagaatg
      481 tagcgcagat ctggctacac agtgcatcca ctcccggcat cgccgatctg gattcctgtt
      541 gtaaaaacgg gatcgcctcc ctcagccagt tatattccac gctgtggtta accggtgcta
      601 aaaacagcgt ttccggcaaa gacgttaaaa aataacgcgc cgaatgttgc aaccgaagag
      661 tgccgcagac aatacaggtc gattcattat cgacatgact caggcgatga gctgaatttt
      721 gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctctcccgtc ggcatttcca
      781 gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgataattcc ccggcaccat
      841 gtggaagctg ccagtcactt cctaacacgc aattcttatc gatcgttcct tgtggagcgt
      901 taagcatcaa caaacggcta agggcatcca tatcagactc ctgagtggtt tttgattaca
      961 aacaccaaca atcttagtaa ataactaatc taataccatc aaatggttaa tcatgatttt
     1021 tgttttgtga ttctgattaa gaaaaagaaa ataccagacg ataagacaaa atatcaagcg
     1081 ttgtgaagaa atggtatttg ctcttttgcg tctatagtca tgatgtcaaa tgaacgcgtt
     1141 tcgacaggaa atcatcatga ataaatatca ggcagtgatt attggttttg gcaaggctgg
     1201 aaaaacatta gccgtcacgc tggcaaaagc aggttggcga gtggctctca tcgaacaatc
     1261 aaatgcaatg tatggcggga cctgtattaa tatcggctgc atcccaacca aaacattggt
     1321 tcatgacgca cagcagcaca cagattttgt ccgtgccata cagcgtaaaa atgaagtggt
     1381 taatttttta cgtaataaga attttcataa tcttgcggat atgcccaata tcgacgtgat
     1441 cgacggccag gcggagttta tcaataatca tagtctgcgt gttcatcggc ctgaggcaaa
     1501 tctggaaatt catggcgaga aaatttttat taataccggt gcacaaaccg tggttccgcc
     1561 aattcctgga attaccacca cgccaggagt atatgacagc accggattac ttaatctaaa
     1621 agaattgcct gggcatttag gtattttggg cggcggatat attggcgttg agttcgcctc
     1681 tatgttcgct aattttggca gcaaggtaac cattttagaa gcagcttcgc tgtttttgcc
     1741 tcgggaagat cgggatattg ctgataatat cgcgacgatt ttacgcgatc agggcgtcga
     1801 tattatcctc aatgcccatg tggagcgaat cagtcaccat gaaaatcaag tgcaagtgca
     1861 tagcgagcac gcccaactgg cggtggatgc actgttaata gcttccggtc gtcaaccggc
     1921 taccgcttcg ttacatccag aaaatgccgg tatcgcagta aacgagcgcg gggcaattgt
     1981 cgttgacaag cgattacata cgaccgcaga caatatttgg gcgatgggag atgttaccgg
     2041 cgggctgcaa tttacttaca tatcactgga tgattaccgc attgtacgtg atgagttact
     2101 gggtgaaggc aaacgtagta ctgatgatcg gaaaaatgtg ccttattccg tatttatgac
     2161 accgcccctg tccagggttg gtatgacaga agaacaagcc agagagagtg gtgctgatat
     2221 tcaggtggtg acattgcctg tagctgcaat tccgcgcgcc agagtgatga acgatacccg
     2281 tggggtatta aaagcgattg ttgataataa aacccaacgt atattagggg catcgctgct
     2341 gtgtgttgac tcccacgaga tgattaatat agtaaaaatg gtgatggatg ccgggctgcc
     2401 ttatagcata ttacgcgatc agatatttac tcatccgtcg atgagcgaat cactcaatga
     2461 tctattttca ttagtcaaat aaactcaaaa tcagacgcca gaacaaatat tctggcgtct
     2521 cagagaaaag aatcttatta attccctgtt actctataac ttcttaatca cttcattgat
     2581 ggtattttat atgtttaaaa aatccgtttt aattgcaaca ctattatctg gcgttatggc
     2641 attttccacc aatgcagatg ataaaataat tctgaaacat atcagcgtct cgtcagtatc
     2701 agcatcaccg acagttctgg aggatgccat tgctgatata gccagaaaat ataatgcttc
     2761 atcctggaaa gtcacatcga tgcgaattga taataattca accgcaacag cagtattgta
     2821 taaataagga tgttcacaat ggaaaaatac ctgcacctgt taagtcgggg agataaaatt
     2881 ggcctgacat tgattcgtct gagtattgca attgttttta tgtggattgg gttattaaag
     2941 tttgtccctt acgaggcaga cagcattaca ccattcgtcg caaacagtcc actaatgtcg
     3001 ttcttttatg aacacccgga agactataaa cagtatctga ctcacgaagg cgaatacaaa
     3061 ccagaagcaa gggcatggca atcggccaat aatacctatg gtttttccaa cggtcttggc
     3121 gtcgtggagg tgattattgc tctgctggtt ttggctaatc ctgtcaatcg ctggttaggt
     3181 ttattgggag ggctgatggc atttaccaca ccgttggtaa cactctcatt tttaatcacc
     3241 accccggagg catgggtacc cgcattgggt gacgctcatc atggtttccc ttatttatcc
     3301 ggtgctggtc gcctggtatt gaaagatact ctgatgctgg caggtgcagt catgataatg
     3361 gcagattcgg cgcgggaaat tcttaaacaa cgcagtaatg aatccagttc aacgttaaaa
     3421 actgaatatt gataatccga acactgtctg ttgttcactt tttcaggcgt caggccccct
     3481 ttctgatgat gaagcctgac gccatattac cggagatatg acagagtgaa gatcaatatc
     3541 actccctgtt aaaaggtttt tgtcgtctta ggtaaaatac atgattaata tcaatcatgt
     3601 atttttacag caagacaacc acgaacaaat tcaggattaa aatgttttac cgactcccca
     3661 acataaccca aatcaagcga actaatttgt gccatttctt tatctgtcag tgagaaatcg
     3721 atttttgatg ggcatctgca atactctgaa gcagactata cggaaaattc cactgtcagt
     3781 ccctccatta ggcatgaaca atgagtctac gttaaaacgt aacctcaaag tagtatgtgg
     3841 attttgatat cacttatgca aaaaattcat taataatgta ggactgaaac ctctctattt
     3901 tcggggacaa cgaagcagac gctaccagtg cttttgcctt cgcccttgct atttttgata
     3961 cacttagggc ccagggtata acgaaaatgt gcgatatgac aaagaattaa cggagaatga
     4021 gatgatcagg cagaagattc tacagcagct cctggagtgg attgagtgca atcttgagca
     4081 ccctatttca atcgaagata tcgcacagaa atctggctac agcagacgca acatccagct
     4141 tctgttccga aatttcatgc atgtgccttt gggagaatac attcgcaaac gaaggctttg
     4201 tcgtgccgcc attcttgtcc ggctcaccgc gaaatctatg cttgatattg cactctcttt
     4261 gcattttgat tcacagcagt cattcagccg cgaatttaaa aagttattcg gctgctctcc
     4321 ccgtgaatac cgccaccgtg attattggga tctcgcaaat atcttccctt cttttttaat
     4381 acgtcaacag caaaagacgg agtgtagatt aatcaacttt cctgagacac ctatttttgg
     4441 caactcattt aaatatgaca ttgaagtgtc gaataaatca ccggatgaag aagtcaaact
     4501 acgacgtcat catttagcca gatgtatgaa gaattttaag acggatatct atttcgtttc
     4561 cacgtttgaa ccgtcaacaa aatcggtcga tttgctcacg gttgaaactt ttgctggtac
     4621 ggtatgcaaa ccaaccgatt cagagatgcc gaaagagtgg acaattaacc gaggactgta
     4681 tgcctctttc cgctatgaag gtgagtggga gcactaccct gaatgggcgc gaaacctcta
     4741 tctgatggaa ttacccgcca gggggttagc cagagtgaac ggcagtgata ttgagcggtt
     4801 ttattacaat gaagatttcg tagaaaagga tagcaatgat gttgtttgcg aaatttttat
     4861 ccccgttcgt ccggtttagt tttcgctata tctaaatgaa tttataacga gactactgtc
     4921 aggaaattca ataaataatg gcgttgaaga tgtcaacgcc attattgttt attagaatgt
     4981 tactttcata ttcttaaaat tatttcatcg cctcctcgtc ctttgccgta tcggcatcca
     5041 ccgcggtaat ggtcaccgtc actggcttca tttcgtaccc gccataggtc agggcgttaa
     5101 atgtgaacgt atagacacca ggcttgctgg tactaaattt acgcgtgatt tttccgctca
     5161 cgaaatggtc atttttcgac ggcaggaact gataatcctt ctccgtcacc ccttccggcg
     5221 tctgaatgtc gacccacata ttcccgccaa cggggttgtt tgccgacatc agtgtcacct
     5281 ccagcttcgc agtcgatttc ccatccgcca ccagagattg catatcagat gatgcggtga
     5341 gtaccgcgtt tttatcatcc gcaacgaatt tagattcggc agttttcgtt gatacctgac
     5401 cgttggtctg actcacttgc gccttgagtg tgtatatcgc cgcaacagta gtggtagcgg
     5461 tgaaaatagc ctgcccttgc tcattcgttg tcgcagtgcc attcggttcc aggtttaacg
     5521 aagccgggct ggcagtcagc gtcacttcgc tatctttcac caaattgtta ttggcatcag
     5581 tcaccgtaac tttatacgtt tgtttggctt tgccgtccgc cagcgcatag ttgcccatca
     5641 cctcaatacc ggagacggtt gcggtagcct tatcggctac aaacgttata gtctgcgtat
     5701 tcgaaggctg gctattcccc gccgatgccg tgatctcata cgttccggca gtcactgaaa
     5761 ccacctgcaa ctctgctttc ccctcatcgt tggctttcac ccagacatta tcgcctgtaa
     5821 gcggcttgac accccggggc agattaaagg tcaccaggct ccctggaaca gggttaccat
     5881 ttttatcctc aacggtcgcc gtcagcgtaa aggcatcttt gccgtttgct actttttgaa
     5941 cggcggtgtc tacctgcagg tttgcctgac cggtgctggc atccgcgttg aatttcaccg
     6001 tgaccgtctt ctgagagttt ttcaccgcgg ccgcgatttc aagttcccct gcaacggttg
     6061 atatcaactc aatatccgct ttacctgccg cattggttgt tactgtattc cccgtcttgc
     6121 tggtcacacc ctgcggtaaa ttcagcgtaa cttcttgccc ctgcaacggg ttaccatagc
     6181 tgtccacgac ggtcagggtt atctggttag cagactgtcc attagccagt tggttattaa
     6241 ccttcactgt catatcacca atctcagcct tagatgcgtc acctgcaaca ttcagcacca
     6301 gtggctgagc aacggcattt tggccgttca ctcgcggcat cacagacaac tgacccgcgg
     6361 cagatcccag cgttaaggtc gacacgtaga ccccattacc tttctctgtc cagtttcctg
     6421 ctgaaggacg ctcactcccc gtgttggcgg caccgctaaa cactggtgca tctggcttca
     6481 tcccggtgac cgggttcccg tacgcatcct tcacggtgaa ggtgagttcc gtcgtcgttt
     6541 tgacggtcgg agccttattg tccgcaacaa gcgtagagtt cgctgatgac atctctccgg
     6601 cgatgaccgt caactgcgcg gcttcggtgg ctgctggctg gccgttgaat agcggcatga
     6661 cacgaagctc gcccgtcttt ccgcctgtag ttaacgtagc aacataggaa ccgtcacctt
     6721 tttcggtcca actggaaacg gtcgcccctt cagaggcggt ccccgtcaaa cttgccgaca
     6781 acgaaagacc actgatagcg ttgccatgcg catctttcgc caccagcgtc acggttgtac
     6841 tttcgccggc ttttacgtga tcttctgcga cagagacttt cgactggttt gaagataacg
     6901 catcagccac cacggttact tttgccgcat ttgctgccgc atcctgtcca tttagcttcg
     6961 gcataacttc taattcaccc gccgtagtgc cgagagaaat ctgcgcagtc caggtcccgt
     7021 cgccattatc cgtccagcct gatgccgtag aacctacagc agctgcgccc gataatgacg
     7081 gtgcatccgg attgagacca gttacagggt tgtcattagc atcttttgcc gtccagattg
     7141 ccgtaactgt accgccaacc accggtttat caggattcag tgtgatggac gaatgtgctg
     7201 catcaagcgg cccggcaaca aacttcagcg tttgttgtaa tgtgcccagc tgatactgct
     7261 cgctgaaagc acgcacaaca acgtttccgg cacgggtcga agaaaccgtg gcgctgtaaa
     7321 cgcctggttt tatttccgaa atggcaccaa cggttacacc attagtgtct tgcggaacaa
     7381 atcgcaagcg gctggcttct cccgtcaccg gattaccctc ggagtccacc gcagtcaacg
     7441 tcaacgtata ggcttgctga ccatcagcaa ccacgtcacc tgacggctcg ttagcgctca
     7501 gggtggagtt tgccacatcc atcatcgtgg cccgcagttc tgcagtgacg gttttgctca
     7561 tgccatcaac actaacagta atcgttgcct cacctgactg cgttccagta gtaaagacag
     7621 actgatacac ccctgcttca gtttcggtga actcacccag tgttggcttt gcctgtgatt
     7681 tagtggcctt cagggtacga gtcacaatat ttccagccgg tttgaaagtt agttcagtct
     7741 tgatctgatc tttcatgccc gtgactggct ggccctcggc gtcgcgcaga gacagcacca
     7801 gcggtctttg ctcattaccg ttagcaagca tttgaatacg gctctgaccg tcaagcgtta
     7861 acgccgtgcg atcggcgctc ataccagctc cggtaatgac cacctctgtc tgcacgcgtt
     7921 ttgaggtatt gcctttgtta tcgtaggcaa ccgcagaaat cgcataataa ttgtctttgc
     7981 ctggacgata agccgggagc gttacttgcc actgactacc ctgaccggta attttgccac
     8041 cttcagccag taatgacggc gcttcccact gcacattttt cagtccgtga gttgctttgc
     8101 tgaccacaag ccccagggaa agtgtctgac cgcccttacc ttcaatacgc tcaggcagag
     8161 caatacggat cacttcagat ttgcggtact caagaacgat gttgttatta cgctcaacca
     8221 ggtcatagcg gctgcctgcc agtacccgac gctcgcgaat gctatccgta tcgagttgtt
     8281 tctccagagg ttcgccaatt cggtagttaa cttccaggcc aaagcgagtg tcattctcac
     8341 cgctcttgcc ctgcttatgc ccggcgctca gtgtcagaag aggcactggc gtataggtca
     8401 cctcggcaga aatagcatgc gggtctttct ggcgcttatc tttaccaaac agcccgactt
     8461 catcgccata atactgttca tacatcaggc ttgcgccaag ctgcggccag gcaggtaaat
     8521 agccctctgc gcggatatcc caaccattcg ccgggcgttc ctgataatcc tcaatatccg
     8581 gcgatttttt ccagccagaa gcccgaatat aaccattggc gctcagtttc agataatcgc
     8641 gccagtattc cgcaccaaca ccaatgcggg tatgactacg ggataaatca tggtcgataa
     8701 aggtgttcac ccccgccatc cagtcatttc ctgaaaaatg acgccagcca aaaccaatat
     8761 ttgactgagt acgatcgtcg gtacgatgta ttgctccctg agtgaacaac atatttgttg
     8821 gcgtatcata aatcggataa agcatttcca gcgaagaatc cttcagcgag aaatctttat
     8881 cgacattcag tttgacgcgc gcagtaccat atttcccgag ccactcctgt atttcctggt
     8941 tagctttagc tgtggccatt ccggtaataa agttacgtgt cgcatcgcta tctggctgac
     9001 tgcttaaaaa tgtcccggca tttgcggcaa acgacgcgac atttttctcc acgttattat
     9061 cagcagttac cgtagtattt cccatgctca accgtggctg aaccgcatgc tgcgcacgtg
     9121 ccgccattac tggggtaaag gtgacagcga gtggaaaaag aacctgaaca gagatatttg
     9181 cccacgccac gcagcgggcc agaactgaat aacgaaatcg tggttgttta tgacctgttt
     9241 tataacgcga cattgagata atttcctgat gaacgaatta acgaacatag gcagacacaa
     9301 ataatggcgt ctgcttgtca gaaaaaccgg ggatttcccg atttcctttt ccgcagaaaa
     9361 ggaaatacct ttatgtaaga aagtgcaccg ccacctaata tatccagtgt ctgtagccac
     9421 tcatcaggtg gttgggcgcg ggattatatt tttatttatg ggtgaaaatt tagctttttg
     9481 tgcaagtcat taaatagaca cctaagttat attttttact ctgcggacag
//
LOCUS       DAZFFI010000156         9378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFFI010000156 DAZFFI010000000
VERSION     DAZFFI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.156"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..1486
                     /gene="cdgI"
                     /locus_tag="ACHJ12_004457"
     CDS             11..1486
                     /gene="cdgI"
                     /locus_tag="ACHJ12_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4992149.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1667..3152
                     /gene="dgcJ"
                     /locus_tag="ACHJ12_004458"
                     /pseudo
     CDS             1667..3152
                     /gene="dgcJ"
                     /locus_tag="ACHJ12_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
     gene            3195..3698
                     /gene="yeaK"
                     /locus_tag="ACHJ12_004459"
     CDS             3195..3698
                     /gene="yeaK"
                     /locus_tag="ACHJ12_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4992150.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3699..3803)
                     /gene="yoaI"
                     /locus_tag="ACHJ12_004460"
     CDS             complement(3699..3803)
                     /gene="yoaI"
                     /locus_tag="ACHJ12_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4992151.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3973..4419
                     /gene="yeaL"
                     /locus_tag="ACHJ12_004461"
     CDS             3973..4419
                     /gene="yeaL"
                     /locus_tag="ACHJ12_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4992152.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4376..5197)
                     /gene="nimR"
                     /locus_tag="ACHJ12_004462"
     CDS             complement(4376..5197)
                     /gene="nimR"
                     /locus_tag="ACHJ12_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4992153.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5294..6475
                     /gene="nimT"
                     /locus_tag="ACHJ12_004463"
     CDS             5294..6475
                     /gene="nimT"
                     /locus_tag="ACHJ12_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4992154.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6530..6877
                     /gene="yeaO"
                     /locus_tag="ACHJ12_004464"
     CDS             6530..6877
                     /gene="yeaO"
                     /locus_tag="ACHJ12_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4992155.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6899..7153)
                     /gene="yoaF"
                     /locus_tag="ACHJ12_004465"
     CDS             complement(6899..7153)
                     /gene="yoaF"
                     /locus_tag="ACHJ12_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4992156.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7336..8361
                     /gene="dgcP"
                     /locus_tag="ACHJ12_004466"
     CDS             7336..8361
                     /gene="dgcP"
                     /locus_tag="ACHJ12_004466"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO4992157.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8394..8492
                     /gene="yoaK"
                     /locus_tag="ACHJ12_004467"
     CDS             8394..8492
                     /gene="yoaK"
                     /locus_tag="ACHJ12_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4992158.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8495..8569
                     /gene="yoaJ"
                     /locus_tag="ACHJ12_004468"
     CDS             8495..8569
                     /gene="yoaJ"
                     /locus_tag="ACHJ12_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4992159.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8628..8876)
                     /gene="yeaQ"
                     /locus_tag="ACHJ12_004469"
     CDS             complement(8628..8876)
                     /gene="yeaQ"
                     /locus_tag="ACHJ12_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4992160.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9103..>9378)
                     /locus_tag="ACHJ12_004470"
     CDS             complement(9103..>9378)
                     /locus_tag="ACHJ12_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4992161.1"
                     /translation="EYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQV
                     CGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2338 a         2244 c         2170 g         2626 t
ORIGIN      
        1 attcggaaaa atgattcagt caacacgtat ttccatgggg ttattcttta aatatttttt
       61 atcgttaacg aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa taaaatccag
      121 cactcacatt gctctccttt ttatggtttc tatgggtaca caaaaattaa aagctcaaag
      181 cttttttatt ttcagtttat tgctgacgtt aattttattt tgcatcacca cattatataa
      241 cgaaaacaca aatgtaaaac tcatcccaca gatgaattac ctgatggttg ttgtggcttt
      301 gtttttcctt aacgccgtca tttttctttt catgttaatg aaatatttca ctaacaaaca
      361 aattttacca acactcattt taagccttgc atttttaagt ggccttatct atttagttga
      421 aaccattgta attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa
      481 tgatgtttct attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc
      541 actcttttgt tatggaaaag acaacatcct tgacaacaat aagaaaaaaa cgggaatcct
      601 gttgctggcg ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta
      661 taacgctgac tattctttgt atgtcgtcga ctactgtccg gacaaccata ctgcgacctg
      721 gggaatcaac tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat
      781 catgcgtaca cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc
      841 gctatgctgc aacttacttc tactgactct ggatgagtat aattacacca tctggtatat
      901 cagtcgcggg attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat
      961 ttttcaggaa ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta
     1021 taatcggcgc tactttttca acagcgtaga gtcattattg tcgcgacctg ttgttaagga
     1081 cttctgtgtc atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca
     1141 tcgtgtgggt gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc
     1201 tgatgatatt ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa
     1261 tagtgcccag gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg
     1321 ctttagtaac agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc
     1381 aacgggtgac acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga
     1441 agcgaaaagc gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc
     1501 agagccatgc tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc
     1561 cctccccagc ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac
     1621 tttatctggt tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca
     1681 tagaatgctc cggcatttta tcgccgcaag tgtcattgtg ctgacatctt ccttccttat
     1741 ttttgaactt gtcgccagcg acagagcaat gagtgcctat ctgcgctata tcgtgcagaa
     1801 agcagactcc tcctttcttt atgataagta tcagaatcag agtattgccg cgcatgtgat
     1861 gcgcgctctc gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga
     1921 ggcttttgag tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg
     1981 cttacgcggc acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc
     2041 actattaccc gcttttggca gtggaaggca accgccacca ggatgattac ggttcaggtc
     2101 ttgggatggc cgaagagaaa tttcactatt atctcgatct caatgaccgc tatgtctatt
     2161 tttatgagcc ggttaatgtt gaatactttg cgatgaataa ctggtccttc ctgcagtcag
     2221 gaagtattgg catcgatcgc aaagatattg aaaaggtatt taccgggcgt accgtattgt
     2281 cgagcattta ccaggatcag cgtactaaac agaacgtgat gagtttgctg acgccggtat
     2341 atgtcgcagg gcagctaaaa gggattgtgc tgctggatat taacaaaaac aatttgcgga
     2401 atatctttta cactcatgac cgccctctcc tctggcgttt tctcaatgtc acgctaaccg
     2461 ataccgattc ggggcgcgac attatcatca accagagcga agataatctg ttccagtatg
     2521 tcagttacgt ccatgactta ccgggcggca ttcgtgtctc gttatccatt gatattcttt
     2581 actttatcac gtcttcgtgg aaaagcgttc tgttctggat tttgacggcg ttaattttgc
     2641 tgaatatggt gcggatgcac ttccgcttat accaaaatgt gtcgcgagaa aatattagtg
     2701 atgcgatgac tggactgtat aatcgcaaaa ttttaacccc tgaactggag cagcggttgc
     2761 agaaactggt gcaatccggt tcttcggtga tgtttattgc aattgacatg gacaagttaa
     2821 agctaataaa tgacaccctc ggtcatcagg aaggggattt agcgattacg ttgttagctc
     2881 aggcgattaa acaatcgatt cgtaaaagtg attatgccat ccgactcggt ggcgatgaat
     2941 tctgcatcat tcttgtcgat tcgacgccgc aaattgcagc acaactgcct gaacgtatcg
     3001 aaaaacgtct gcaacatatc gcgccgcaga aagagatcgg cttctcttcc ggtatttacg
     3061 cgatgaaaga aaacgatacg ttacatgatg cgtataaagc ttccgatgag cgtttatatg
     3121 tcaataagca gaacaaaaac agccgttcat gataaccttc tgtggttgtt tgcttgtaat
     3181 ctcaggagcg tgaaatgact gaaatggcta aaggaagcgt gacgcatcag cggttaatcg
     3241 ctttattatc acaagaaggt gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat
     3301 gtgaagcggt gtcagaaatt cgtggcaccg ctttaggcca gggagctaag gcactggtat
     3361 gtaaagtcaa aggcaatggc gtaaatcaac atgttctggc gatcctcgct gccgatcaac
     3421 aggccgatct gagccaactc gccagccata taggcggatt acgcgcttcg ttggccagcc
     3481 cggcggaagt cgatgaactc acaggctgtg tcttcggcgc gatcccccct ttcagcttcc
     3541 atccaaaact caaactggtt gccgaccctc tactcttcga acgatttgat gaaatcgcct
     3601 tcaatgcagg gatgctggat aaatccgtta ttttgaaaac cacggattat ttgcgcattg
     3661 cgcaaccaga actgctcaat ttccgccgca ctgcgtaact agcctgtccg ttcaataagc
     3721 agaacggaca aaaccagcac aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc
     3781 tcgacgaaca tttgatcgtt catagcatgc ccccgggaga tagctgtttt cacgttattg
     3841 ttagcgtgca caaatggcag tttgatgaca gttcgcctga ttttttatgc aaaaaacgta
     3901 aagattttct acttccttcc tgcagcaagc gtaaagtaag caggcttatt attttttggc
     3961 aaggaaacca cgatgtttga tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc
     4021 tttatcagtc ataacaccac tgtcgccgtt tcaattctgg tgttgatcat tgtccgcgtc
     4081 acaccgttaa gcaccttttt cccgtggatt gaaaaacagg ggctaagtat cgggataatc
     4141 attctgacga ttggcgttat ggcccccatc gccagcggga cgctaccgcc ctcaaccttg
     4201 attcactcgt ttctgaactg gaaatcgctg gtggcaattg cagtaggcgt tattgtctcc
     4261 tggctgggtg ggcgcggcgt gaccttaatg ggcagccagc cgcaactggt cgccgggctg
     4321 ctggtaggca ctgttttagg cgtggcgctg tttcgcggcg taccggtcgg cccgcttatt
     4381 gccgcgggtc tggtttcgct gattgtgggg aaacagtagt taatcccgca atatatcggc
     4441 ctggcgtttg ccccaggcct ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc
     4501 ccagcgtatg agcaactttc tgtacagtat cgcctttcac taatccctga agtgccataa
     4561 tcagctgtaa ctgctgacgc cactggcgaa aactcaaccc cgtctctttt actatcagtc
     4621 gggcaagatt gcgttcactc atcgcaaaaa agccagccca ttgccctaat gacccccact
     4681 cgacgggccc tttcgccatc atctccacca tcgtgcggat tttaggatga gaagaaacgg
     4741 gcaggtgcag ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc
     4801 tgcgggtcat cggctcagca cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt
     4861 cccggcataa gggcgatatt ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg
     4921 gttcgataaa cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc
     4981 cgcccggtat ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac
     5041 aggtaattgc accatgtaat gctagtatca gctgtccttt gcggtgctga tgtacaggac
     5101 tggttaactc atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt
     5161 cgggttcata gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt
     5221 tgtcatttta gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa
     5281 caatgagaag catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat
     5341 cgctggcatt ctgatgattg ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt
     5401 actggatacg attcgttccg cttactcgct gacgacagcg caaaccggct tattaaccac
     5461 cctgccatta ttggcctttg cgctaatctc acctttggct gccccggtgg cgcgacgttt
     5521 tggtatggaa cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg
     5581 ttctctccct tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc
     5641 attaggcaat gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag
     5701 acttaccggc gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatggt
     5761 cgtgccgctg gctttgaacg gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt
     5821 tcctctgctg gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt
     5881 gagtacctcg cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt
     5941 cacattgttt cttgggatca actcactggt ctattacgtg attattggct ggcttccggc
     6001 gatcctcatc agtcgcggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca
     6061 actagccaca gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca
     6121 gcgtggtatt gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt
     6181 tatgccagcg cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat
     6241 gatactgggg ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact
     6301 ctcggggatg gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctgatggg
     6361 taaaatacac gatgctaacg gtgactggtc cgtaccactg atgggtgttg ccatactttc
     6421 actactgatg gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc
     6481 ggtgctatag tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca
     6541 gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg
     6601 cctctggccg cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat
     6661 cacgccgtca acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt
     6721 tcgcgagcaa tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga
     6781 catcgccaaa aaacagccgc tgaccctgct ctacgcagca aaaaacacca cgcagaacca
     6841 tgcgctggtg ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt
     6901 atttttcacc agccggatgg tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc
     6961 ccggtaattt gcaattgttg ctgacccctt gcgctgtctg cactggaatg agcgtcccgc
     7021 ccttctgctg gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggtg
     7081 cttctggctg agaagaggtt gaacaaccag ccaggaccag caagcaaggc aaagcaaaac
     7141 tgataatttt catttattga tctcacacat ttatctacgc ttagagtatc gcggtatcgt
     7201 tttgttttgc agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc
     7261 tttgttgatg atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta
     7321 tcctggagtt gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa
     7381 gaacagtcac tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat
     7441 atggaatcaa cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt
     7501 gcccgtaaca gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg
     7561 ttatgcaaac gcgctattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg
     7621 ggcgactgta ttgcggcacg caatcttgga atcaccacat ttctgagtac accaattcac
     7681 ttaccggatg gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg
     7741 agtgaacgcg cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa
     7801 aaagaggcgt tggttgaaca gctgcgcgaa gccaatgccg cgctgattgc gcaatcgtat
     7861 accgactcgt taaccgggct accgaatcgg cgggcgattt ttgaaaatct gacgacgctg
     7921 ttttctctcg cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac
     7981 ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg atctgtttct cattcaggtt
     8041 ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat
     8101 gagtttttgg ttgtttcact aaataacgag aatgcggata tttcgtcgct gcgagaacgt
     8161 attcaacagc aaatacgtgg agaatatcac ttaggtgatg ttgatttgta ttatcccggt
     8221 gccagtcttg gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat
     8281 gctgccgata ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg
     8341 catccagcgc tacattcctg aggcgtattc acatcctttt gattggtgat aacatgcgaa
     8401 tcggtattat ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt
     8461 ttattggcgg ctatgctgcc ccgggagcat aaagatgaaa aaaacaacga ttattatgat
     8521 gggtgtggcg attattgtcg ttctcggcac tgagctggga tggtggtaac gtcacctcta
     8581 aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc
     8641 ttcctgtaga taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa
     8701 ttgaagccat cgactttacc aaagccaaac agtgtgctga tccatccgcc gactacggca
     8761 ccgactatcc ccagcagaat agtcataaag aatccaccac catctttacc tggcatgatc
     8821 cacttcgcca gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt
     8881 tcctcactta tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat
     8941 cgggagggca agatttgtga cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt
     9001 gcgccatcaa cgatcggtga taattaccaa ccacaaacat catgttcgtt ttccgtgtct
     9061 tgacatcctc cacgccctga atgacgagga tccctgctac gttcaggctg tcgcctgaat
     9121 catttcggtg ggttcctgct tcaacgagcg gcctgactgc accatctctc cacaggcaag
     9181 aacggcgtgc cccgccgcta aaatgttacg agcgccgttt acatcggcgt tcgctgtata
     9241 tccacatacc tggcatctga attgactttg tgacaggcga ttttctttcg ctgtatgacc
     9301 acagcacgcg caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg
     9361 ccagagctgc ttgtactc
//
LOCUS       DAZFFI010000157         9211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFFI010000157 DAZFFI010000000
VERSION     DAZFFI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.157"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..995
                     /gene="ybeF"
                     /locus_tag="ACHJ12_004471"
     CDS             42..995
                     /gene="ybeF"
                     /locus_tag="ACHJ12_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4992162.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYITQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1204..2169
                     /gene="lipA"
                     /locus_tag="ACHJ12_004472"
     CDS             1204..2169
                     /gene="lipA"
                     /locus_tag="ACHJ12_004472"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4992163.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2270..2473)
                     /gene="tatE"
                     /locus_tag="ACHJ12_004473"
     CDS             complement(2270..2473)
                     /gene="tatE"
                     /locus_tag="ACHJ12_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4992164.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2602..3390)
                     /locus_tag="ACHJ12_004474"
     CDS             complement(2602..3390)
                     /locus_tag="ACHJ12_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4992165.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3483..3866
                     /gene="crcB"
                     /locus_tag="ACHJ12_004475"
     CDS             3483..3866
                     /gene="crcB"
                     /locus_tag="ACHJ12_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4992166.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3921..4130)
                     /gene="cspE"
                     /locus_tag="ACHJ12_004476"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3921..4130)
                     /gene="cspE"
                     /locus_tag="ACHJ12_004476"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4992167.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4305..4865)
                     /gene="pagP"
                     /locus_tag="ACHJ12_004477"
     CDS             complement(4305..4865)
                     /gene="pagP"
                     /locus_tag="ACHJ12_004477"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4992168.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5454..6839
                     /gene="dcuC"
                     /locus_tag="ACHJ12_004478"
     CDS             5454..6839
                     /gene="dcuC"
                     /locus_tag="ACHJ12_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4992169.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6880..7560)
                     /gene="dpiA"
                     /locus_tag="ACHJ12_004479"
                     /gene_synonym="citB"
     CDS             complement(6880..7560)
                     /gene="dpiA"
                     /locus_tag="ACHJ12_004479"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4992170.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(7529..9187)
                     /gene="dpiB"
                     /locus_tag="ACHJ12_004480"
     CDS             complement(7529..9187)
                     /gene="dpiB"
                     /locus_tag="ACHJ12_004480"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4992171.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         2355 a         2318 c         2082 g         2456 t
ORIGIN      
        1 tttaaatatt atttttccat gaataaaatt ggagtaagtg cgtggatagt aataatcaaa
       61 ttgaaccctg tttaagcaga aagtcgtcag aaggtaaacc acaaatattt acaaccttac
      121 gaaatattga tcttaacctt ctgactattt ttgaagctgt atatgtacat aaagggatcg
      181 ttaatgcagc gaaagtgctt aatctgaccc cctcggcaat cagtcagtct attcagaaac
      241 tgcgcgttat attccctgac ccattgttta ttcgcaaagg ccagggtgtc actcctaccg
      301 catttgcgat gcatctacat gagtatatca ctcagggcct tgagtccatt cttggcgcgc
      361 tggatatcga aggaagctat gataagcaac gaacgataac tattgcgacc actccctcgg
      421 tcggagcact ggtccttcct gtcatctatc gggcgattaa aactcactat ccgcagcttt
      481 tactgcgcaa cccgcccatc agcgacgcgg aaaaccaact cagtcagttt caaaccgatc
      541 tcatcatcga taacatgttt tgcaccaatc gtacggtgca acatcatgtt ctgttcaccg
      601 acaatatggt gttaatttgc cgcgagggaa atccactact ctctttagaa gatgacagag
      661 agactatcga caacgctgcg catgtacttc tgttaccgga agagcaaaat ttcagcggtc
      721 tgcggcagag agttcaagaa atgtttccgg accggcaaat caatttcacc agctacaaca
      781 ttttgacaat cgctgcactg gttgccaaca gtgacatgtt agcgattatt cccagccgtt
      841 tttataacct gtttagccgc tgctggccgc tggaaaaact gccttttccg tccttaaatg
      901 aggagcaaat agatttctct atccactaca acaaattcag cctgcgtgat cccatcctgc
      961 acggagtaat agatgttatc cgtaatgcat tttgaaaaag tattgttaca gccagatcac
     1021 agcgcagaac gataaaatgg cacaatccaa caacaatttt acattttcgc gaccgctttg
     1081 gctgctttca ggtccgtttc aatgatatac tgccagtcgt taattcaaaa atagttgata
     1141 attacaacaa tctattgaat tgaaacgctt tccttcgtaa ttcgcaactg gaacacgcac
     1201 gctatgagta aacccattgt gatggaacgc ggtgttaaat accgcgatgc cgataagatg
     1261 gcccttatcc cggttaaaaa cgtggcaaca gagcgcgaag ccctgctgcg caagccggaa
     1321 tggatgaaaa tcaagcttcc agcggactct acacgtatcc agggcatcaa agccgcaatg
     1381 cgcaaaaatg gcctgcattc tgtctgcgag gaagcctcct gccctaacct ggcggaatgc
     1441 ttcaaccacg gcacagcaac gtttatgatc ctcggcgcta tttgtacccg ccgttgtccg
     1501 ttctgtgatg ttgcccacgg tcgcccggta gctcctgatg ccaatgaacc agtgaaactg
     1561 gcgcagacca ttgccgatat ggcgctgcgt tatgtggtta tcacctccgt tgaccgtgat
     1621 gacctgcgcg atggcggtgc ccagcacttt gcggattgca ttactgccat tcgggaaaaa
     1681 agcccgcaaa tcaaaattga aactctggtg ccggatttcc gtggtcgtat ggatcgtgct
     1741 ctggatattc tgactgcaac gccaccagat gtgttcaacc ataacctgga aaacgtaccg
     1801 cgtatttacc gtcaggtacg gcctggtgca gattacaact ggtcgctgaa gctgctggaa
     1861 cgctttaaag aagcgcatcc ggaaatcccg accaagtctg gtctgatggt gggactgggt
     1921 gaaaccaatg aagaaattat tgaggtaatg cgcgacctgc gccgtcatgg tgtgacgatg
     1981 ttaacgctgg ggcaatattt gcagccaagc cgccatcacc tgccggttca acgttacgtt
     2041 agcccggatg agttcgacga aatgaaagcc gaagcgctgg cgatgggctt tacccatgct
     2101 gcatgcggtc cgtttgtccg ctcttcttac cacgccgatt tgcaggcgaa agggatggaa
     2161 gttaagtaat cctgtaataa ttacttacat ctgtctgata aaaaacccgc tttttgaagc
     2221 gggttttttg tatcgaacaa gatattgagg gagcgtcctg ctcgccacgt cactctttat
     2281 gagagagctt ttcagcctga agatcaacgt ctgcgccttt tttcgccgca gcatcgtcat
     2341 cattcatcgc cttcttgaac cctttaatgg ccgctccaag gtctccgccc agcgtacgta
     2401 acttcttagt cccaaacagc agaacgacca gcgccgcaac taccagcagt ttggtaatac
     2461 taatctcacc catagatacc ttcttgacat ataaccggca acagaacgcg ccttaaaaac
     2521 cagagaatat taacggtcat tcgacgcgcg cacacaatag caatctgtaa caaggtgaat
     2581 caagcgccga gtagaaaaac atcataataa ttgcggcggc gcaaagcgac ggttgtttaa
     2641 gacgggcagt tgcgcgcgca cctgacgcac acgttcgggc gtcacttccg ccataatgag
     2701 cgcaggcatt tctgacgctg ccgcaatggt gacgccaaag ggatctataa tccggctttg
     2761 accgatattt ttgttcccgc actcccccgc cgccaccata taacaggtgg tatccagcgc
     2821 acgagcggca agcaacgttg accagtgatg ctctttgagc ggcccgcgaa cccaggcggc
     2881 aggaagtacc aggatttcag ctccctgtaa tgcctgtgcc agcgccagct ctggaaagcg
     2941 taagtcataa caggtcatca gaccgacctt catcccctcc acctcgagta acggagcgat
     3001 ttcattacca gcatcaacac ggcgtgattc ctgaatggca aatgcatcat agagatgcaa
     3061 tttggcataa cgggcgacga tgtttcctgc ctgtagcgcc accagcatat tccatgcgcg
     3121 ccccggcgtt gaaggaacat gaatcgtcag aattgtcgtc atcatgttac gtttactttc
     3181 tcgccgtaaa cgtccgagga attcgccttc cagcagctgt gctgatttaa ccgatagatc
     3241 tgcatcatga tcatcgcgcg ccagcaatgc ttccggcagg acgaacagcg atacgtcgtt
     3301 ttccgccgcc tgcgccatca acgaggcaca aatctcagcg ttcttttccc acacagatgt
     3361 aacagcaaac tgtcctgcgg caaccagcat tgctacctcc tgtcagagtc gtaaaattcg
     3421 ccaacgacga ttattcggcg ttacacttat cactcataca aatcaaatag caggattttg
     3481 cagtgttaca acttctttta gcagttttta ttggcggtgg tacgggaagc gtggcgagat
     3541 ggctgttaag tatgcgattt aacccgctgc atcaggcgat tccgttgggg acgctggcag
     3601 caaacctgat tggggcattc atcataggaa tgggattcgc atggttcagc aggatgacga
     3661 acattgatcc agtgtggaaa gtattaatca ccaccggatt ttgtggcggt ctaacaacct
     3721 tctcaacatt ttcggcagaa gtggtatttt tgttacaaga gggtcgcttt ggctgggcat
     3781 tactgaacgt tttcgtcaac cttctggggt cttttgccat gacagcactg gctttctggc
     3841 tgttttcggc ctcaaccgca cactaaagac caaaaaaaaa cccgctgatt aagcgggttt
     3901 tgaattcttg ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca
     3961 ccgttagtga tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc
     4021 tggattgcag agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg
     4081 aatcctttgg actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc
     4141 tttacatgaa aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc
     4201 tttgtccagt gcaactttac taaaaagtga taaaagtcgc gttatttttg atgttactta
     4261 ttttagctat tgattttaaa gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc
     4321 atccaggcaa agtacacatt gccattgttg taggtacccg gaatgtaggt catctgaaaa
     4381 gtcactgggc cataacccac ggaggccaat ggcagtagaa ccgggagagg gatgtaattc
     4441 cagttatcgc gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc
     4501 gccagcggtc gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac
     4561 gagtccttaa atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac
     4621 aggccaaaac cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa
     4681 gcgaaacgtg catgccaggt gatggcagga atatataaat cataatgttc aggctgttgc
     4741 caggtttgtg caatattttc tctaaacgtt gtcatccact catctgcgtt agcaaaaact
     4801 ttaccaacgc tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg
     4861 ttcatttgtg accataaaac atttatcaaa aatctactac tagcatagca aagctacaat
     4921 taacataacc ttaatagacc caacataaag aataatctga atagctgctc tatttatttc
     4981 cgcttgttca agttaaatat ttccgcgcat cgattaaaga tgatgcgtcg ttttctgcga
     5041 tgggaatagt caaaaaagaa aaaccaagtc ttttttgatg acaaaatgca atcaaggaaa
     5101 aattatttta ttttttaact agatacaaca atcatcttaa cgaagtatat aatataaatc
     5161 gtcaacctat gctcatgtta gattattttt tgagcgcaca gccgcagcta acaccattgc
     5221 aattaacaaa tttgcatcaa tccaccatca atttacacac attattatgt gataattgcc
     5281 aaccgctaaa tatgcgtttt gttatctatg tataaaaaca gcaacttcaa tgtcttaatg
     5341 gcagtttttc ttgattttaa tcagcattca tcgccaattt attgggcata ttttttcctt
     5401 aagctttagg aattttttat tatttacttt ggggcctgga gacaggaaaa attatgctga
     5461 cattcattga actccttatt ggggttgtgg ttattgtggg tgtagctcgc tacatcatta
     5521 aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg attatcagtg
     5581 ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac agcgccacgg
     5641 atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc ggcatgatga
     5701 ttatgatgct gtgtggattt gccgcttaca tgacccatat cggcgcgaat gatatggtgg
     5761 tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg atgattgccg
     5821 cctattttgt cgcctgtctg atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt
     5881 tgctgatggc aaccctattt ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg
     5941 ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc
     6001 tggcggcgca agcttccgaa atgtcgctga ttgacttcgc cttcaaaacg acgctgccta
     6061 tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa cgttatctgg
     6121 ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc accactgctc
     6181 ctgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt tttgacggta
     6241 aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg attgcctcca
     6301 ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg gaagtggctt
     6361 atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc gctggggtat
     6421 tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc gctacctcgt
     6481 ttggttcagc gagtatcatc ctgatgctgg tattggtgat tctgaccatg ctggcggcag
     6541 tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg
     6601 cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc
     6661 tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa
     6721 tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg ctagtgattg
     6781 ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca ggcaagtaaa
     6841 tagtaatgcc ggaggcaagt ttcctccggc tttttcatgt cacccactgt ggtatatacg
     6901 ttgcggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc ggctggcgca
     6961 atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg ccaccgtttc
     7021 tgccgtatgt tgcacaccag gctctttgaa cagttttcgc accgcgttta gcgtcaaagg
     7081 gtcaatgccg gtcggtagct cgtccttagg ttcaccgcga gcataagcat taaacatctc
     7141 atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac gctggcggaa
     7201 acgggttagc gtttgcccca gccgttcata ggcaatgggc ttaatgagat aatcaaatac
     7261 accacaacgt acggcttcag acaccgtttc catatcgctg gctgcagtgg taaacaccac
     7321 gtcgccggga taatgcgcct gcaccagttc atgcagtaaa ttaatccctc taccgtcagg
     7381 aagatagtta tcgagcaaga ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg
     7441 cgccaggttt cccgccagta atatctgact gaatccggga atgtgacgaa tatattccgc
     7501 atgcatctct gccagcggcg tttcgtcctc aacgatcaat agggttaatg gagctgtcat
     7561 taggtttcac tttcggaata tagattgaaa ataaggtacc gcagggatca ttatcttcga
     7621 gagtgataac accaccgcag cgcgttacgt agctggcaat caagtacaac ccaatgccat
     7681 gttcaccggg ctcgtcagca cgcgtactga ccccctgctc aaatattttg tctcgtagag
     7741 actctggaac gccgcagccc tgatcggcga cttcaatcac cacatcatcg ccttcatcgc
     7801 tgaggaataa ttcaacgatc ttgtttcctt catcgctacg caggctggct tcgaaggcgt
     7861 tatcaagtaa attgccaaca atggcggcaa actcggtgct atccagtcct ggcggcagtt
     7921 gcgaaagctg gctaccgggg acgatgatca tttttagccc cagttcgcgg gcgcgctgca
     7981 ctttaccaaa aagcagcccc gccacctggc gatcggcaaa cgcctcgcgc aggctgtcaa
     8041 taagctgttg ctgggcctga gactccccct gcaccatcgc cagcacgcga tcatactctt
     8101 tcatctgcaa cagaccattg agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac
     8161 gaaggctctc aacgtattgt tttatttgcg tcagttgcgc attgagggtg gaaatttcgt
     8221 ctttactacg aaagctgata atggccccca gcaaatcatc acctgaacga atagcttccc
     8281 ggttggcaat aacgctcaga ccgttaaagt tagccaccac atcctgacgt ttttcatcaa
     8341 tctgttcggt aaagaaatcg gcgggcctga ccacttcagc aatgggttta cccaaccatt
     8401 gccgtccggg agagctcagc cccagcatct ttcttgcgtt acgattgatg gcggtaatgt
     8461 aaccatgcgg atccaccgca atcagccctt cataaaccga actaaacagc gcctcttgct
     8521 gacggaccac gcgtgcgatt tgctttggct ccatgcccat catttgccga cggatatgcg
     8581 ctgccaggaa ccacgacagc aacatcagaa tccctaacag cacgacaaac acgcctgcca
     8641 tcggtaataa aaactcagcc cgccagctat cgattttact caccaggtag ccaatcgaca
     8701 ccacgccgat gacttttcca tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc
     8761 ccattgaccc tttcccggta atgaagtagc tctccccttt ctccagcgcg ccctgtttgg
     8821 tgaactgcat aggataacca attttctccg gattaggatg gtaaaggcgg atcgagtgcc
     8881 ggtccccaat caccacataa tcaaaatcgg tatctctttg taatttgtta gcgatggtcg
     8941 ccagccgttt gtagtcacgc gttttcaccg cagagatgac actgtcattg gaggcaataa
     9001 ttttcgcctg attcattgcc atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg
     9061 ccgtaaaata ttgcgccagg gctgcaatga caaatattga gaacaccaga atcagcaaaa
     9121 agatacgcag cggaaatgcc agtctttgga aaaatgcaaa ctgtttattc tcgttaagct
     9181 gcaacattgt tttccttgcg ggttgtcccg a
//
LOCUS       DAZFFI010000158         9002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFFI010000158 DAZFFI010000000
VERSION     DAZFFI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.158"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..572
                     /gene="uspC"
                     /locus_tag="ACHJ12_004481"
     CDS             144..572
                     /gene="uspC"
                     /locus_tag="ACHJ12_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4992172.1"
                     /translation="MSYSNILVAVAVTPESQKLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(579..2003)
                     /gene="otsA"
                     /locus_tag="ACHJ12_004482"
     CDS             complement(579..2003)
                     /gene="otsA"
                     /locus_tag="ACHJ12_004482"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4992173.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1978..2778)
                     /gene="otsB"
                     /locus_tag="ACHJ12_004483"
     CDS             complement(1978..2778)
                     /gene="otsB"
                     /locus_tag="ACHJ12_004483"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4992174.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2945..3931)
                     /gene="araH"
                     /locus_tag="ACHJ12_004484"
     CDS             complement(2945..3931)
                     /gene="araH"
                     /locus_tag="ACHJ12_004484"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4992175.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3946..5460)
                     /gene="araG"
                     /locus_tag="ACHJ12_004485"
     CDS             complement(3946..5460)
                     /gene="araG"
                     /locus_tag="ACHJ12_004485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4992176.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5530..6519)
                     /gene="araF"
                     /locus_tag="ACHJ12_004486"
     CDS             complement(5530..6519)
                     /gene="araF"
                     /locus_tag="ACHJ12_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4992177.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKSAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7316..7819
                     /gene="ftnB"
                     /locus_tag="ACHJ12_004487"
     CDS             7316..7819
                     /gene="ftnB"
                     /locus_tag="ACHJ12_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4992178.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7898..8149)
                     /gene="yecJ"
                     /locus_tag="ACHJ12_004488"
     CDS             complement(7898..8149)
                     /gene="yecJ"
                     /locus_tag="ACHJ12_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4992179.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8264..8350)
                     /gene="azuC"
                     /locus_tag="ACHJ12_004489"
     CDS             complement(8264..8350)
                     /gene="azuC"
                     /locus_tag="ACHJ12_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4992180.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8612..8935
                     /gene="yecR"
                     /locus_tag="ACHJ12_004490"
     CDS             8612..8935
                     /gene="yecR"
                     /locus_tag="ACHJ12_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4992181.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2326 a         2275 c         2054 g         2347 t
ORIGIN      
        1 tgatcaccgg gagtgattga ttaattgtct ttactaataa tcagactaat atttacctgt
       61 ttgaccgagt tgggattgcg tcgtttctcc attaggagta aagctttaat gtcacctgaa
      121 gttcacagaa taaggaacag gttatgagct atagcaatat tcttgtcgct gttgcggtta
      181 caccggaaag tcagaaactg ctggcaaaag cggtatctat cgccaggcca gtaaagggac
      241 acatcagttt aattactctc gcttccgacc cggaaatgta caatcaatta gccgcgccga
      301 tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca aagctttctt gataagttaa
      361 ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag ttaagcgaac
      421 atattctgga agtatgtcac aaacatcatt tcgatttggt gatttgcggt aatcacaatc
      481 acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca agtgaggtcg
      541 atgtgctgtt agttccactt acgggtgatt aaccgctcct acgcaagctt tggaaaggta
      601 gcaactttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt taggtcgcta
      661 atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag catttctgca
      721 tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc agctgcaact
      781 tcgtcacgat cgtaggggtt aacaattaac gccgacgtta actcgtttgc cgctcccgca
      841 aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcagcaac atactctttt
      901 gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga gtagcggaat
      961 attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg cgtccagcct
     1021 aattgcccgt atttaccatt aattcgtcca gcttcatttt cgagctgatg acgaatatcc
     1081 tgataggctt gcacatcacc acgcgacgtt ggtgcaatct gggtataacg aattttacca
     1141 tgatgctgcg gatatttttc cagcaacgct tcataggcga gaaaacgctc tggcaaacct
     1201 ttggaataat ccagccgttc gacagaaaag atattttgta cgtttttcag ttccgcttta
     1261 agttgcgcca gttttggcgg cagtggcccg gcagcctgtt tggctatttc tttcggttca
     1321 atgccgatcg ggtagacttc tgttcgaaat gctttgcccc aggctgtatg gctttttgcg
     1381 ctacgtgtcg tgacgcgggt caggttagaa agacaatcca ggaacgccag acgatcgttt
     1441 tctgtctgga aacccagcaa atcataatca caaagctgtt caagcaaggt gtcatatgtc
     1501 ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat gcagaaagaa accaatgcga
     1561 ttattcactc cccgtttgcg taattcatgc gcaaatggca acaggtgata atcgtggatc
     1621 cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa cgcatttacg
     1681 cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg ataatgaaaa
     1741 gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc ctgttcgctg
     1801 aggttaaaag aggcccacgt aatgttacct tttttcacct tttttagcgg ctgatcctca
     1861 ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag tgcccccagt
     1921 atgccaacgg caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc aatccggtta
     1981 gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt tttgttgtaa
     2041 tgcggtggtt atcatttcaa gccagctcca gacatccggc acacccgcca gtcgccatga
     2101 tgcctgagtt gcacctgtgc caatttttac tgacattccg cccagtcggt taacgactgc
     2161 gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc cgataaaggg
     2221 agcttcctgc ataaaagctg caattgcctc acctttactg gtacctctcg gtttgatctc
     2281 gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa tacgttgcgc
     2341 taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg caaaagccat
     2401 ccctttcgcc tccagctccg cgccgggata ctgagcgatg actgtatgca gttgcacgct
     2461 aatatcacgc gcaatcgcat ccggcagatg aacgatatgt gttttaccat tgatgtcacg
     2521 gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca gtgcgtcaag
     2581 ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg ttgccagtag
     2641 ctgtagtcct tgcagaatat tgtcaggcac gacgacctga tcgggatgcg gtttgatttc
     2701 cgccagcgtt ccatcaagat caaaaaacca ggcatatttc gcggatagtt caggggtttc
     2761 ggttaacggt tctgtcaccc ggctctcctc cttctctttt cttattgttt tgcgcagaac
     2821 gcgctttaca gacatgtaag tatagacagt gtgacggggg tcgccatttg caatgaagaa
     2881 cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac gttaaattgt tgtggaaaaa
     2941 agcatcagac agtgcgtttc gctttttgct tgtaacggtc gaagatcacc gctgccagca
     3001 ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg ttcatggcgt
     3061 tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt ccgatgccac
     3121 ctttcagaga aacgccacct aaaacgcagg cggagataac aataagctca taaccaatcg
     3181 acgtcattgg ctgcccacta gtcatacgtg aagccagaat aattccggct atcgcagata
     3241 ccaggccaga gagaacaaag ataataattt tggtgcgaac aaccggtaca cccgccagac
     3301 gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg accaaaggtg gttttattca
     3361 gcaacaaacc aaagataatc agacacgcga cggtgagcca gattggcgca ggcagaccga
     3421 accagttagc gtaaccaagg gcaaagaagc tttcatcttc gataccgacc gctttaccgt
     3481 ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtt gtgatcagag
     3541 catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga atgccaagca
     3601 gcaaccccgc tgccacgcca atccacaggc tttcagtcag gttgataacc accgccgtgg
     3661 tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg gaagcaaggc
     3721 agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc aaccctttca
     3781 tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag agcaccgcaa
     3841 acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac gacttaggtg
     3901 cgccagaccc cgatgtagaa acagaagaca tcatactctc cttactcagg caactgcctg
     3961 gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct cgtgtaacaa
     4021 ttcaccggcg atttcacctt cccgcatcac cacaatccgg tcggcaacgc cgaggacttc
     4081 aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc gccgccagcg cataaatcac
     4141 gttgtaaatt tcatgcttag caccaacatc aatgccgcgc gtaggttcat ccagcaaaat
     4201 gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat ttccgcctga
     4261 gagattcatg atcagttgct cagcgcccgg cgttttgatg ttgagcgaac gaatgtggtg
     4321 atcggcattg ttttcttccc aaccgttgtt gattacacaa ccgccgagca catgtttacg
     4381 tctggcactg atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc cttccgcttt
     4441 gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt tacgaatatc
     4501 gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc caaacaagcc
     4561 tttcattaat tcgctacgcc ccgctcctac cagaccaaac agcccaacaa tttcaccact
     4621 gcgaaccgcc agacttattg gcgtacgcac gcctggtgct ttcacagcat caagacgcag
     4681 gcgctcctcg ccataactac gaggttgcca gccgtagata tcgccaatgt cgcgcccgac
     4741 catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg ttttgacata
     4801 acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt ccatacgatg
     4861 agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc ggaaaagatt
     4921 gtcgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa ttttagcgtt
     4981 acgcgccagc gccttggcga tttcaaccat ctgccactga ccaatggaga gatatttcag
     5041 cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg cctcataatt
     5101 cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat agatgttttc
     5161 cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca cacccgcatt
     5221 aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta cagaacccgt
     5281 ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg cgccattttc
     5341 acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt
     5401 aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag actgttgcat
     5461 aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaga ggggaatttt
     5521 tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa agttgtcacg
     5581 cgtgatcagc accacgtcgg taacttcggt aaattttggc ggttcaacgt cttttgctac
     5641 ccagttgtaa agcatttcgc tggatttata gccatgtacg tccgggcttg gcagcaggga
     5701 accgtagaag ccggttgcct gtgctttaga cagttcgctc accgcatcca caccgttaat
     5761 gccaatgccg atgatatcgg cagctttaaa gccctgacct tccgtcgcgc gtacgccgcc
     5821 cagcacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg gatgttgaac
     5881 cagcattgag ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg taggtacctg
     5941 ataaatttgt ttttccggga atccggccga tttcagcgca tccatagaac ccgttgtacg
     6001 gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc accgcgcttt ctttgacatc
     6061 ccagccacgt ttctgcatct ctttatacag ttcctgaccc tgacgttcgc caattttagt
     6121 cgccgccatc atcaccagcg gaacggtatc cattggctta cctttggcgt taacaaactg
     6181 gtcatccacg gcaatgactt tcatatcgta gccacgcgct ttcgcgacga tggcagagcc
     6241 gagtttgggg tccggagtac aaataacgaa accttttgcg ccactggcag ccaggctgtc
     6301 gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct caaaccctaa
     6361 atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct cttccggttg
     6421 cttcaccaga aaaccgagct tcaggttctc cgccatagcg gattgtgaca taacggctgc
     6481 cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca gctttagtgt
     6541 cgttttgtgt agggcaaaaa cgaatgactt tcgttaaatt aatcggaaaa caaagcatta
     6601 ccttttatct aaaagataag tgaccgtgtt gacatagttt tagcgagaaa ttaattctcc
     6661 ataggagagc aatatcacat cgcagaatta cagtgagaac gtgcataaat ttagcgggaa
     6721 aagacataag ggaaagccaa tttgtcagac aaattgtcga atgcacagca gattaatcca
     6781 taagattagc ctggaaatcc ttgttgtctt tggtacccat gcgggatgtc ttctttttaa
     6841 ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat aaccgcaact
     6901 caaagatgtg gaaaatgcac gtcattcatt tcgccattaa ttatcactgt gctcattaat
     6961 taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt tataaaatca
     7021 tcacttcatc atgaattcaa attcattgat taatatcaat aagatacaaa aagcactatc
     7081 attaaaattc attgcagtta cattgatttc atcaatgaaa tgtaaaaata tataaacttg
     7141 atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga tcatcgtcag
     7201 aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt
     7261 atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc
     7321 aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa
     7381 tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt
     7441 ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa
     7501 gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa aactgaactc
     7561 tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta gtacgttggc
     7621 acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga
     7681 tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg atgaagtgcg
     7741 cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt
     7801 gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg aaggttttga
     7861 gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg
     7921 taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg accgggtctg
     7981 cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt ttttcacgaa
     8041 cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag
     8101 caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct
     8161 gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca
     8221 ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc
     8281 ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc
     8341 gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat
     8401 aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt
     8461 taatgttata aaaatgataa tcaaaaaaca gccccctatt tctgacacct acagatggca
     8521 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcactgta acgcgtaaaa
     8581 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     8641 aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag ttgacgcggc
     8701 aacaggtatt gtacggttag tttatgacca ggcttttttg cagcatgccc ataccgatcg
     8761 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcggt
     8821 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     8881 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     8941 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     9001 tc
//
LOCUS       DAZFFI010000159         8767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFFI010000159 DAZFFI010000000
VERSION     DAZFFI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.160"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHJ12_004491"
     CDS             complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHJ12_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4992182.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            716..934
                     /locus_tag="ACHJ12_004492"
     CDS             716..934
                     /locus_tag="ACHJ12_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4992183.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            942..1883
                     /gene="ribF"
                     /locus_tag="ACHJ12_004493"
     CDS             942..1883
                     /gene="ribF"
                     /locus_tag="ACHJ12_004493"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4992184.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1926..4742
                     /gene="ileS"
                     /locus_tag="ACHJ12_004494"
     CDS             1926..4742
                     /gene="ileS"
                     /locus_tag="ACHJ12_004494"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4992185.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4742..5236
                     /gene="lspA"
                     /locus_tag="ACHJ12_004495"
     CDS             4742..5236
                     /gene="lspA"
                     /locus_tag="ACHJ12_004495"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4992186.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHJ12_004496"
     CDS             5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHJ12_004496"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4992187.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5775..6725
                     /gene="ispH"
                     /locus_tag="ACHJ12_004497"
                     /gene_synonym="lytB"
     CDS             5775..6725
                     /gene="ispH"
                     /locus_tag="ACHJ12_004497"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4992188.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6791..7705
                     /gene="rihC"
                     /locus_tag="ACHJ12_004498"
     CDS             6791..7705
                     /gene="rihC"
                     /locus_tag="ACHJ12_004498"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4992189.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7872..8693
                     /gene="dapB"
                     /locus_tag="ACHJ12_004499"
     CDS             7872..8693
                     /gene="dapB"
                     /locus_tag="ACHJ12_004499"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4992190.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2018 a         2167 c         2477 g         2105 t
ORIGIN      
        1 tttacaagat ctaataagga gatatttatg aatggttaaa gtgtatagga atttaattca
       61 aaaatagagt ggtcttaagt ttaatacctc aatgtgtatc acaatatcca cattctttgt
      121 aggggagtct ggagattgag tagatattct tgttcagaat gtatcagccg atggttctac
      181 gattcttaag ccacgaagag ttcagaaagt gtaacggcat gtctgcaaaa gccagcatct
      241 tttgactatc tggcaaccgg tagtgtgttc tctcacgcat cataaaagca gcaggcataa
      301 aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca aattggcgat taagccagtt
      361 tgttgatctg tgcagtcagg ttagccttat gacgtgcagc tttgtttttg tggatcagac
      421 ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag
      481 ctttgtcgcc agcttcgata gctgcgtata ctttcttgat gaaagtacgc atcatagagc
      541 gacggcttgc gttgtgctta cgagcctttt cagactgaat ggcgcgcttc ttagctgatt
      601 tgatattagc caaggtccaa ctcccaaatg tgttctatat ggacaattca aaggccgagg
      661 aatatgccct tttagccttc ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg
      721 ccggcactcg ttacgtagtg atggcgcagg attctaccag cttgcggggt gtgaatacag
      781 cttttccgcg ataaaaattg cagcaggcgg tcactttctt cccgtgattt gcgccatggc
      841 aatgaaaagc cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt
      901 acaaggtata ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca
      961 tacataatct cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg
     1021 gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca
     1081 acttaccggt gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata
     1141 aagccccggc acggctgacc cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg
     1201 ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt
     1261 tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt
     1321 tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt acagaaagct ggcatggaat
     1381 acggcttcga tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca
     1441 ccgctgtgcg tcaggcgctg gcggatgaca atctggctct ggcagaaagt ttactggggc
     1501 acccgtttgc catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt
     1561 tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg
     1621 tagaagtgct gggcctcggt gaaaagccgt tacccggcgt ggcaaacatt ggaacacgcc
     1681 caacggttgc cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc
     1741 tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg
     1801 cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt
     1861 ttgggctaac aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga
     1921 atctgatgag tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg
     1981 gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg
     2041 gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt
     2101 atgcgaatgg cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg
     2161 tgaagtccaa agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg
     2221 gtctgccgat cgagctgaaa gtagagcaag aatacggtaa gccgggtgag aaattcaccg
     2281 ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca
     2341 aagactttat ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact
     2401 tcaaaactga agccaacatc atccgcgcgc tgggcaaaat catcggcaac ggtcacctgc
     2461 acaaaggcgc gaagccagtt cactggtgcg ttgactgccg ttctgcgctg gcggaagcgg
     2521 aagttgagta ttacgacaaa acttctccgt ccatcgacgt tgcttttcag gcggtcgatc
     2581 aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa cggcccaatc tcgctggtga
     2641 tctggaccac tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt
     2701 tcgactatgc gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg
     2761 aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg
     2821 cggagcttga gttgctgcgc tttacccatc cgtttatggg cttcgacgtt ccggcaatcc
     2881 tcggcgatca cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg
     2941 gcccggacga ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc
     3001 cggacggcac ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag
     3061 cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa
     3121 tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacgc
     3181 cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc gcagtcactg aaagagatca
     3241 aaggcgtgca gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gttgctaacc
     3301 gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc
     3361 acaaagacac ggaagagctg catccgcgta ctctcgaact aatggaagaa gtggcaaaac
     3421 gcgttgaagt tgacggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg
     3481 atgaagctga tcagtacgtg aaagtgccgg acacattgga tgtatggttt gactccggat
     3541 ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt
     3601 atctggaagg ttctgaccag caccgtggtt ggttcatgtc ttccctaatg atctccaccg
     3661 cgatgaaagg caaagcgccg tatcgtcagg tactgaccca cggctttacc gtggatggtc
     3721 agggtcgcaa gatgtctaaa tccatcggca ataccgtttc gccgcaggat gtgatgaaca
     3781 aactgggcgc ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg
     3841 ccgtttctga cgagatcctg aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg
     3901 cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc aaaagatatg gtgaaaccgg
     3961 aagagatggt ggtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca
     4021 tcctcaaggc gtacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct
     4081 gctccgttga gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca
     4141 aagcggacag tgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gcagaagcgc
     4201 tggtgcgctg gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctacctgc
     4261 cgggcgaacg tgaaaaatac gtcttcaccg gtgagtggta cgaaggcctg tttggcctgg
     4321 cagacagtga agcgatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag
     4381 tgaacaaagt cattgagcaa gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg
     4441 cagtaacctt gtatgcagaa ccggaactgt cggcgaaact gaccgcgctg ggcgatgaat
     4501 tacgatttgt cctgttgacc tccggcgcta ccgttgcaga ctataacgac gcacctgctg
     4561 atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg
     4621 agaagtgccc acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag
     4681 aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct
     4741 gatgagtcaa tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct
     4801 gattatcgat ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt
     4861 cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt
     4921 ccttgccgat agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag
     4981 cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc
     5041 ttatgcgctg attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt
     5101 cgttgtcgat atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaaccttgc
     5161 cgatactgcc atttgtgtcg gtgcggcact gattgtgctg gaaggttttt tgccttctaa
     5221 agcgaaaaaa caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag
     5281 gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca
     5341 gagcaatagc gccgtcctgg tgcacttcac gctaaaactc gacgatggca ccaccgctga
     5401 gtctacccgc aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttctga
     5461 agggctggag caacacctgt tggggctgaa agtgggcgat aaaaccacct tctcgctgga
     5521 gcccgatgcg gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga
     5581 atttatggat gcaggcgagc cagaaattgg cgcaatcatg ctttttaccg caatggatgg
     5641 cagtgagatg cctggcgtga tccgcgaaat taacggcgac tccattaccg ttgatttcaa
     5701 ccatccgctg gctgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc
     5761 actggaggcg taacatgcag atcctgttgg ccaacccgcg tggtttttgt gccggggtag
     5821 accgcgctat cagcattgtt gaaaacgcgc tggccattta cggcgcaccg atatatgtcc
     5881 gtcacgaagt ggtacataac cgctatgtgg tcgatagctt gcgtgagcgt ggggctatct
     5941 ttattgagca gattagcgaa gtaccggacg gcgcgatcct gattttctcc gcacacggtg
     6001 tttctcaggc ggtacgtaac gaagcaaaaa gtcgcgattt gacggtattt gacgcgacct
     6061 gtccgctggt gaccaaagtg catatggaag tcgcccgcgc cagtcgccgt ggcgaagaat
     6121 ctattctcat cggtcacgcc gggcacccgg aagtggaagg cacgatgggt cagtacagca
     6181 acccggaagg gggaatgtat ctggttgaat caccagacga tgtgtggaaa ctgacggtca
     6241 aaaacgaaga gaaactctcc tttatgaccc agaccacgct gtcggtggat gacacgtctg
     6301 atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca
     6361 tctgctacgc cacgactaac cgacaggaag cggtacgtgc cctggccgaa caggcggaag
     6421 ttgtgctggt ggtcggttcg aaaaactcct ctaactccaa ccgtctggcg gagctggccc
     6481 agcgtatggg caaaagcgcg tttttgattg atgatgcgaa agatatccag gaagagtggg
     6541 tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc
     6601 agaatgtggt ggcacgtttg cagcagctgg gcggtggtga agccattccg ctggaaggcc
     6661 gtgaagaaaa cattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg
     6721 attaagtcat tagcagccta agttatgcga aaatgccggt cttgttaccg gcatttttta
     6781 tggagaaaac atgcgtttac ctatcttcct cgatactgac cccggcattg acgatgccgt
     6841 cgccattgcc gccgcgattt ttgcccccga actcgacctg caactgatga ccaccgtcgc
     6901 gggtaatgtc tcggttgaga aaaccacccg caacgccctg caactgctgc atttctggaa
     6961 tgcggagatt ccgctcgccc agggagccgc tgtgccgctg gtacgcgcac cgcgtgatgc
     7021 agcatctgtg cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa
     7081 gccaatcggg ataccagcgt ttctggcgat tcgtgatgcc ctgatgcgtg caccagagcc
     7141 tgttaccctg gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc
     7201 ggaatgtaaa ccgtatattc gtcgtctggt gatcatgggc ggttcttctg gtcgcggcaa
     7261 ctgtacacca aatgccgagt ttaatatcgc tgccgatcca gaagcggctg cctgtgtctt
     7321 ccgtagcgga atcgaaatcg tcatgtgcgg tctggatgtc accaatcagg caatattaac
     7381 ccctgactat ctcgctactt tgccggagtt gaatcgcacc ggaaaaatgc ttcatgcact
     7441 gtttagccac taccgtagcg gcagtatgca aagcggtctg cgaatgcacg atctctgcgc
     7501 catcgcctgg ctggtgcgcc cggacttatt caccctcaag ccgtgttttg tcgcggtaga
     7561 aactcaaggc gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa
     7621 accagccaat gtaaaggtgg cattggatct ggatgtgaaa ggcttccagc agtgggtggc
     7681 tgaggtgctg gctctggcgt cgtaacctgt cacatgttat cggcatgcag tcattcatcg
     7741 actcatgcct ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt
     7801 ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa
     7861 agagaatagc tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta
     7921 tgggccgcca gttgattcag gcggcgctgg cattagaagg cgtgcagctg ggcgcggcgc
     7981 tggagcgcga aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga
     8041 aaacaggcgt taccgtgcaa agcagcctcg atgcggtaaa agatgatttt gatgtgttta
     8101 tcgattttac ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca
     8161 aagggatggt gatcggcact acggggtttg acgaagccgg taaacaggcg attcgtgacg
     8221 ccgccgccga tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc
     8281 ttaagctgct ggagaaagca gccaaagtga tgggtgacta caccgatatc gaaattattg
     8341 aagcacatca tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg
     8401 cgatcgccca cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc
     8461 acaccggtga acgtgtgcct ggcaccattg gttttgccac cgtgcgtgca ggtgacatcg
     8521 ttggcgaaca taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg
     8581 cgtccagccg catgacattt gctaacggcg cggtaagatc ggctttgtgg ttgagtggta
     8641 aggaaagtgg tctttttgat atgcgagatg tgcttgatct caatagtttg taaccacaaa
     8701 atatttgtta tggtgcaaaa ataacacatt taatttattg attgtaaagg gctttaattt
     8761 ttggccc
//
LOCUS       DAZFFI010000160         8698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFFI010000160 DAZFFI010000000
VERSION     DAZFFI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.161"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            51..1469
                     /gene="traH"
                     /locus_tag="ACHJ12_004500"
     CDS             51..1469
                     /gene="traH"
                     /locus_tag="ACHJ12_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO4992191.1"
                     /translation="MNLFLSPTLKHKLCRNRGGIPALLLCSCLSFIPFTAVADVNSDM
                     NQFFNKLGFATNTTQAGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGI
                     DAYLGSFSFINGEQLQRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINS
                     MNLSSCQVAQGIVGGLFPQTQESQQKVCQDIAGESNIFADWAASRQGCTVGGQSSAVR
                     DKASDKDKERVLKNINIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPA
                     RTTDRDIVRAIMEGGTAKIYHCNDSDKCLKVVADANVTISKDRALKSQIAKLLTSIQN
                     KALSDSPLAENEKTFIQSTTIPVFRYLVDPLMLGVSNSVLYQLTDYIGYDIMLQYIQE
                     LLQQARAMISTGNYPQAVLDSVLDNLNQAQQQIAIFQSQVQVQQDALLVVDRQMSYMR
                     QQLSARMLSRYQNNYHFGGGVQ"
     gene            1466..4288
                     /gene="traG"
                     /locus_tag="ACHJ12_004501"
     CDS             1466..4288
                     /gene="traG"
                     /locus_tag="ACHJ12_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO4992192.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMVADDPYMVIFSRPSPLRGVYDSNNNFVSCKDVSVTLK
                     DKLNLDTKSGGKTWHYYVQQLFGGRPDPDLLFRELLSDSYSYFYGASQSASQIMRKNV
                     TINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSVGHVIMRNLPMVQTILTG
                     ITIGIFPLLVLAAAFNKMTLPVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASSSITPTAS
                     AAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRDGSMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATRELASRSTRGSAGMY
                     GDAHAEWGVKPKILGVGGGAGVRGGGRTSIDWNDEDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQDSNIRND
                     VKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            4304..4807
                     /locus_tag="ACHJ12_004502"
     CDS             4304..4807
                     /locus_tag="ACHJ12_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO4992193.1"
                     /translation="MIMKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMTFIIGTLYELRRVCGCV
                     SVREWMQSQVNENTNEELHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            4839..5570
                     /gene="traT"
                     /locus_tag="ACHJ12_004503"
     CDS             4839..5570
                     /gene="traT"
                     /locus_tag="ACHJ12_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO4992194.1"
                     /translation="MKTKKLMMVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5630..5890
                     /locus_tag="ACHJ12_004504"
     CDS             5630..5890
                     /locus_tag="ACHJ12_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992195.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            6058..6789
                     /locus_tag="ACHJ12_004505"
     CDS             6058..6789
                     /locus_tag="ACHJ12_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992196.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYSPVIQDNTATTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAKEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            6845..>8698
                     /gene="traD"
                     /locus_tag="ACHJ12_004506"
     CDS             6845..>8698
                     /gene="traD"
                     /locus_tag="ACHJ12_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO4992197.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQ"
BASE COUNT         2247 a         2000 c         2326 g         2125 t
ORIGIN      
        1 gtttttaatt agttttaata aacgcagtgt tttctgacag aggaccgttt atgaatctgt
       61 ttttatcccc gacgcttaaa cataaattat gcagaaatcg aggtggcatt ccagctttat
      121 tgttatgcag ttgcctgagc tttataccct ttactgcagt tgcagacgtc aacagcgaca
      181 tgaaccagtt cttcaacaag ctgggctttg ccaccaatac cacccaggcc ggagtctggc
      241 aggggcaggc tgccgggtat gcctatggcg gttctctgta tgcccgtaat caggtcagaa
      301 atatccagct tatctccatg acactgcccg acatcaatgc agggtgtggt ggcatcgatg
      361 cctatctcgg ctcattcagt tttattaatg gcgagcagct gcagcgtttt gttaagcaga
      421 ttatgagtaa cgcgatgggg tacttttttg accttgccct gcagacaacg gtgcccgaaa
      481 tcaaaaccgc gaaagacttc ctgcagaaga tggcgaatga cattaacagt atgaacctca
      541 gtagctgtca ggtggcccag gggatagttg gcgggttgtt tcctcagacg caggagtcgc
      601 agcagaaggt ctgccaggat attgcaggtg agagcaatat cttcgctgac tgggccgctt
      661 cccgtcaggg gtgcacggtc ggagggcagt cttctgcggt gcgtgacaag gccagcgata
      721 aggacaagga gcgggtactg aaaaacatca atatcatctg ggactcgctg tccaaaaaca
      781 ggatgcttga tggtaacaaa gagctgaaag agttcattat gacgctcaca ggtacgctga
      841 tttttggtga agacagtgag attacccctc ttcctgcacg aaccactgac cgcgatattg
      901 tccgggcgat tatggaaggg ggtaccgcga aaatttatca ctgcaatgat tcggataagt
      961 gcctcaaagt ggtggccgat gcgaatgtga ccatcagcaa ggacagggcg ctcaagtcac
     1021 agatagcgaa actgctcacc agtattcaga acaaggcgct cagtgattct ccgctggctg
     1081 agaacgaaaa aaccttcatt cagagcacca ccattcctgt tttcagatat ctggttgacc
     1141 cactgatgct gggcgtatcc aacagtgttc tctaccagct gacggactat atcggttacg
     1201 acatcatgct ccagtacatt caggaactgc ttcagcaggc ccgggcgatg atttccaccg
     1261 ggaactaccc gcaggcggtc ctggacagtg tgctcgataa ccttaatcag gcgcagcaac
     1321 agattgccat tttccagagc caggtgcagg tccagcagga tgcactgctg gtcgttgacc
     1381 gtcagatgag ctacatgcgc cagcagttgt ctgcccgtat gctcagccgt taccagaata
     1441 attatcactt cgggggaggc gtacagtgaa cgaggtctat gtcatcgccg gtggggaatg
     1501 gttacggaac aacctgaatg ccatcgctgc ctttatgagt acccggacgt gggattccat
     1561 cgaaaaaatt gcgctcacat tatctgttct cgcagtggct gtgatgtggg tacaacggca
     1621 caacgtgatg gatttgctgg gatgggttgc cgtgtttgta cttatcagtc tgctggttaa
     1681 tgtccgcaca tcggtgcaga ttattgataa cagtgacctg gttaaggttc accgggtgga
     1741 taatgtgccg gtcggactgg cgatgccact ttcactgaca acccgtatcg ggcatgcgat
     1801 ggtggccggc tatgagatgg tgttcgctca gcctgacagc gccacctaca gcaagacagg
     1861 catgctgttc ggggcgaacc tgattgtgaa aagcaccgat ttcctgtccc ggaatccgga
     1921 aatcatcaat ctgtttcagg actacgtcca gaactgcgtg ctgggtgata tttacctgaa
     1981 ccacaaatac acgctggaag acctgatggt ggctgatgac ccctatatgg tgattttttc
     2041 ccggcccagc ccgctgcggg gggtatatga cagtaataat aattttgtca gctgcaagga
     2101 tgtgtcggtc acgctgaaag acaagctgaa tcttgataca aagagcggag gcaagacctg
     2161 gcattattat gtgcagcagt tatttggcgg cagaccggac cctgacctgt tattcaggga
     2221 actgctcagt gacagttaca gctatttcta cggggccagc cagtcggcca gtcagattat
     2281 gcgtaagaat gtgacaataa atgcactgaa agagggtatc accagtaatg cggcccgtaa
     2341 cggtgatacg gccagcctgg tgagtctggc caccacctca tcaatggaga aacagcgact
     2401 ggcgcacgtc tctgtcggac atgtaataat gcgaaacctg ccgatggtcc agaccattct
     2461 gaccggaata accattggta ttttcccgct tctggtactg gctgctgcat ttaataaaat
     2521 gacactgccg gtgctgaagg gctatgtgtt tgcccttatg tggctgcaga catggcctct
     2581 gctgtacgcc attctgaaca gcgccatgac attttatgcg aagcagaacg gtgccccggt
     2641 ggtgttgtcg gaaatctctc agatacagct gaaatactcc gacctggcat caaccgcagg
     2701 ttatctttca gcaatgatcc cccctctgtc atggatgatg gtacgaggcc ttggtgccgg
     2761 attttccagc gtatacagcc actttgcgtc ttcttccatc acgccaaccg ccagtgcggc
     2821 aggcagtgtg gttgacggta attactccta cggcaacatg cagacgaata acgtgaacgg
     2881 cttcagctgg agcaccaaca gtaccacgtc atttggtcag atgacgcacc agaccggtag
     2941 tggtgcaacc gccacgcaga cccgtgacgg cagtatggtg atggacgcca gtggggcaat
     3001 gtcccgtttg ccggttggta ttaatgcaac gcgtcagatt gcagcagcgc aacaggaaat
     3061 ggcccgggag tcgatgaata aggcggaaag tgccctgcat ggtttcagca gcagtattgc
     3121 cagtgcctgg aacacactca gccagtttgg ctctaaccgg ggaagcagtg attcagtcac
     3181 cagtggcgct gacagcacga tgagcgcgca ggactccatg atggccagcc gtatgcgcag
     3241 tgcggtggaa agctatgcga aggcgcataa tatcagcgat gagcaggcga caagagaact
     3301 ggcatccaga agtacgagag gttctgcggg gatgtatggt gatgctcatg ctgaatgggg
     3361 ggttaaacct aaaattcttg gtgttggtgg tggcgcaggt gtcagaggtg gtggtaggac
     3421 aagtattgac tggaatgatg aggatgcgca tcaggccagc agtggttcac gggccagcca
     3481 tgatgcccgt catgatattg atgccagagc tacacaagac tttaaagaag caagcgatta
     3541 ctttaccagt cgcaaggtca gtgaatccgg cagccatact gacaataatg ccgattccag
     3601 ggtggaccag ctttctgcag ccctgaactc agcgaaacag agttacgacc agtacacgac
     3661 gaatatgacc cgcagccatg aatatgctga aatggcctcc cgtactgaaa gcatgagtgg
     3721 gcagatgagt gaagacctgt cacaacagtt tgcacagtat gtgatgaaac acgcgccgca
     3781 ggatgcagaa gctattctga caaacaccag ttcaccggag attgcagaac gtcgtcgggc
     3841 aatggcctgg tcttttgttc aggaacaggt acagcctggt gttgataacg cctggcgtga
     3901 atcccgtggc gatatcggta aaggaatgga gagcgtacct tcgggcggtg gcagccagga
     3961 tattattgca gaccatcagg ggcatcaggg tattattgag caaagaacgc aggacagtaa
     4021 tatccgtaac gatgtgaagc agcaggttga taatatggtc acagaatatc ggggcaatat
     4081 cggggatact cagaacagca tccacggcga ggaaaatatt gtaagagggc agtattctga
     4141 actgcaaaat caccataaaa cagaggcatt atctcagaat aataaatata atgaagaaaa
     4201 atcagctcag gaaggaatgc ccggtgctga cagcccacag gaacttatga acagggcaaa
     4261 agagtatcag gataagtata agcaataatg ctgggtttgg gagatgataa tgaaaaattt
     4321 agctcatgtt gctctgataa ctgtgataca gctcattgcc tgtaatctgg ctgattgggg
     4381 gggcgctgag acagttttta tcctgtgctt tattgtacta tggcagggat tgtttatatg
     4441 gttgttttct caaatacgaa aaaaacggca tgtgtcggat gagtttaaat tcagtaaagg
     4501 cgtttggtat atactgatgc cagtatcttc tttattctct ccgttattaa gcctgatgac
     4561 ttttatcatt ggtacactat acgaattaag gcgagtttgt ggttgtgtaa gtgtaagaga
     4621 atggatgcaa tctcaggtaa atgaaaacac caatgaagaa ttgcatctgg attttgatga
     4681 tgtgaatttt gattatgtgg atttttatcg tactaatccg gcaacaggat tagctatgca
     4741 tggaggattt gattcagcag gaaatacttt tgggacttgt tggcaggact atgatgatag
     4801 gcgataagaa aactgtagct tgtaataaag gtaatttgat gaaaacaaaa aaattgatga
     4861 tggttaccct ggtcagttcc actctggtcc tttcagggtg tggtgcgatg agcacagcaa
     4921 tcaagaagcg taatcttgaa gtcaaaactc agatgagtga aaccatctgg ctggagcctt
     4981 ccagtgaacg caccgttttc ctgcaaatca aaaacacatc tgacaaggat atgagtggat
     5041 tgcaggcaaa aatcagtgat gccgttaaag ccaaaggcta ccaggttgtg acctcacctg
     5101 acaaggcata ctactggatc caggccaatg tcctgaaggc agacaaaatg gatttgcggg
     5161 agtcccagac ctggctgaac agcggttatg aaggtgccat tgccggtgca gcattgggca
     5221 ccggcatcac aggatataac tccagctctg ccggtgcggc gctgggtgta gggattgcca
     5281 cagggttgat tggtatggct gccgatgcaa tggtggagga tatcaactac accatgatca
     5341 cggatgtgca gattgcagag cggacaacat ctaaagtcag aactgataat gttgctgctc
     5401 tccgccaggg aacctccggt gcaaaagtgc agaccagcac atcaaccgga aatcaacata
     5461 aataccagac ccgtgtggta tctaatgcca acaaggttaa cctgaaattt gaagaagcga
     5521 agcctgtact ggaagaccag ctggctaaat ccatcgcgaa catcctgtaa attacccatc
     5581 cggcccggac agattgtacc gggccgatat tcctgccccg gatgaataca tgaaccgtgc
     5641 agaatctcgt ctctcgcagg tactctttct gctgggctat gacagtgaaa agtatcctga
     5701 gcatgtatgt cgggagttat taaaagaact ttaccggttt gctgatgcaa ccggagattc
     5761 tgaactggcc atagcctgcc agagatacct gacagcactg gaaccgttct ctgcatatga
     5821 gatcggcgta agcgcaacag aatatcttcc tgtgagtgaa tatccgaccg gacgtcgcag
     5881 aagaaaatag ctgacgcctc gcgttgctcg ttgtccaacc ccggaaagta acaaaataaa
     5941 ttttgtcact tcccggcgtt tccgaaataa atatgagaac actgaacggt tgtgcaaatt
     6001 ctttgtgcga ccgtgaagtg ttgccggcga agccggaagc ttccggagaa agtcattatg
     6061 acatcagaca atatcgctga atatgttggt gagaataatc aatttactga gaagcctgta
     6121 actgaatact ccccggtgat acaggataat acagcgacca caaaagatta tctgtacagc
     6181 aataatattg atactgacct tttagtggtt atcggagggc ttattgttat attgtttctc
     6241 atgatgcttg tcatgtactt tattaattct ctcaggcata aggaattaac tgaaatactg
     6301 acaaaaatat cttttcctga gaaatccggg agcaatccgc ttaagccgga agagggccgc
     6361 tgtaatttgt tgtccctgtg tgtgctggcc ccggtattct gtacccccaa aaaactggca
     6421 gaagtctcca gacagatcat cgtgaccagt gcgaaggaaa ttgcctggac gcccgggagt
     6481 agccgtattg atggtgagca ggaagaaaat attacctggt ctctgtcgtc acagactcat
     6541 gccgataaat acgatctcat caataaaaca tcccgtgttt ctcctgacag tggccacttt
     6601 tatgaaccgg gcacggctct ggattatcgt gagcgggtac gcctttttgt tcagatccag
     6661 tattcacacg agagtgactg gtctgaaata ttgcgcctga ctgatatcgt gctggaaaaa
     6721 gtttcttccg gcagaccagc aggcggctat caccaccggt atacagggta tacatggttt
     6781 gttctgtgaa tgtattccac accgctcttt tctgctctgt acctgacttt accggagttt
     6841 catcatgagt tttaacgcaa aggatatgac ccagggcggt cagattgcgt ccatgcgtat
     6901 ccgcatgttc agccagatcg ccaatatcat gctttactgc ctgtttattt ttttctggat
     6961 cctcgttggt ctggttttat gggtaaaaat aagctggcag acgtttgtga acggctgtat
     7021 ttactggtgg tgcaccacgc tggaaggcat gcgggattta atcaggtccc agccggtcta
     7081 tgagatccag tattacggca aaaccttccg gatgaacgcg gctcaggtac tgcatgataa
     7141 atacatgatc tggtgcggtg agcagctatg gtctgcattc gttctggcgt ctgtcgtggc
     7201 cctggttatt tgcctgatca ccttctttgt tgtctcctgg attctggggc gtcagggtaa
     7261 acagcagagt gaaaatgaag tcacaggtgg tcgtcagctg actgacaatc cgaaagaggt
     7321 tgccaggatg ctgaaaaaag acggcaagga ctccgatatc cggattggcg atctgccgat
     7381 tatccgggat tctgaaatcc agaatttctg tctgcacggc acggtcgggg ccggtaagtc
     7441 ggaagttatc cgtcgtctgg ccaactacgc ccgtcagcgc ggtgatatgg tggtgattta
     7501 tgaccgatca ggggaatttg ttaaaagtta ctatgacccg tccatcgata atatcctgaa
     7561 tccgctggat gcccgctgtg ccgcctggga tttgtggaag gagtgcctga cacagccgga
     7621 ttttgataat acggcaaata ccctgatccc gatgggcaca aaagaagacc cgttctggca
     7681 gggctcagga cgcaccattt ttgcggaagc ggcgtacctg atgcgtaatg accccaaccg
     7741 cagctacagc aaactggtgg atacactact ctccatcaaa attgaaaaac tgcgtacctt
     7801 cctgcgtaac tcaccggcgg ccaacctggt ggaagagaaa atcgagaaaa cggcgatttc
     7861 catccgtgct gtgctgacca actacgtgaa agccatccgt tacctgcagg ggattgagca
     7921 taatggtgag tccttcacca tccgtgactg gatgcggggt gtccgtgaag ataagaaaaa
     7981 cggctggctg tttatttcgt cgaatgccga cacccatgct tccctgaaac cggtgatctc
     8041 catgtggctg tccatcgcca ttcgtggcct gatggcgatg ggggagaacc gtaaccgtcg
     8101 tgtgtggttc ttctgtgatg agttacccac gttacacaaa ctgccggacc tggtggagat
     8161 cctgccggaa gcccgtaagt tcggtggctg ttatgtgttc ggtatccagt cctatgccca
     8221 gctggaagat atctacggtg agaaatcggc agcctcgctg tttgacgtca tgaacacccg
     8281 tgcctttttc cgttccccca gccataagat tgcagagttc gctgccggtg aaattggtga
     8341 gaaagagcac ctgaaagcca gcgagcagta ttcctacggt gctgatccgg tacgtgacgg
     8401 ggtatcgacc ggtaaggata tggagcgcca gacgctggtc agttattccg acattcagtc
     8461 tctgccggat ctgacctgtt atgtcaccct gcccggaccg tatccggcag taaaactctc
     8521 cctgaaatat caggcacgac cgaaggttgc tccggagttt attccgcgtg acatcaatcc
     8581 tgaaatggag aaccgtctga gtgccgtact tgccgcaagg gaagcagaag gtcgtcagat
     8641 ggccagcctc ttcgaaccgg atgtcccgga ggttgtttcc ggagaagacg tgactcag
//
LOCUS       DAZFFI010000161         8526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFFI010000161 DAZFFI010000000
VERSION     DAZFFI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.162"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..112
                     /locus_tag="ACHJ12_004507"
     CDS             <1..112
                     /locus_tag="ACHJ12_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992198.1"
                     /translation="AAKVLKRVKFIPQGIQQQVPAKRQNRGSRNSTGDVL"
     gene            331..561
                     /locus_tag="ACHJ12_004508"
     CDS             331..561
                     /locus_tag="ACHJ12_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992199.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            613..1974
                     /locus_tag="ACHJ12_004509"
     CDS             613..1974
                     /locus_tag="ACHJ12_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4992200.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFAR
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLNTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2021..2584
                     /locus_tag="ACHJ12_004510"
     CDS             2021..2584
                     /locus_tag="ACHJ12_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4992201.1"
                     /translation="MHSQLRERIRLMRARLDNARPVIEIPAEFQLFVTPAPVCDRLVT
                     LAEISNHDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTRVPTMIIRLRA"
     gene            2584..2820
                     /locus_tag="ACHJ12_004511"
     CDS             2584..2820
                     /locus_tag="ACHJ12_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992202.1"
                     /translation="MTQGRREDKCPSVRDGKRPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLFFFLAL"
     gene            2736..2990
                     /locus_tag="ACHJ12_004512"
     CDS             2736..2990
                     /locus_tag="ACHJ12_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992203.1"
                     /translation="MSVTDDVVVPATATATATATALLFPGALILTTAVLSCLPGAPSA
                     PGCLEASGRQARLRQAVAGAPASLRRTRWRGFGLSLRSDA"
     gene            3463..3984
                     /locus_tag="ACHJ12_004513"
     CDS             3463..3984
                     /locus_tag="ACHJ12_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992204.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKDGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            4041..4274
                     /locus_tag="ACHJ12_004514"
     CDS             4041..4274
                     /locus_tag="ACHJ12_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO4992205.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNRYIRDFGILK"
     gene            4335..6293
                     /locus_tag="ACHJ12_004515"
     CDS             4335..6293
                     /locus_tag="ACHJ12_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4992206.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLVSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYCVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKYRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPQPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            6348..6782
                     /gene="psiB"
                     /locus_tag="ACHJ12_004516"
     CDS             6348..6782
                     /gene="psiB"
                     /locus_tag="ACHJ12_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4992207.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFYLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            6779..7498
                     /locus_tag="ACHJ12_004517"
     CDS             6779..7498
                     /locus_tag="ACHJ12_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4992208.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            7495..7809
                     /locus_tag="ACHJ12_004518"
     CDS             7495..7809
                     /locus_tag="ACHJ12_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO4992209.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            7959..8111
                     /locus_tag="ACHJ12_004519"
                     /pseudo
     CDS             7959..8111
                     /locus_tag="ACHJ12_004519"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            8056..8178
                     /locus_tag="ACHJ12_004520"
     CDS             8056..8178
                     /locus_tag="ACHJ12_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992210.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1906 a         2298 c         2611 g         1711 t
ORIGIN      
        1 tgcggcgaag gttctcaaac gggtgaagtt cataccacag ggaatacagc agcaggttcc
       61 cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg
      121 atttatttca ggctccggca atacgcattt atgtgttctg tcagtcatcc ggctggcagg
      181 taatgagaac gcagaaacag aaggaggaaa acaccggaga acgtcagacg cagatatgaa
      241 aaaagcaggt cagggacctg cttaatccac accgcaaaaa ctgttcgcgc agtttctgcg
      301 gttccattca acagtcataa ggatgcattt atgacagtaa gtattgtttc accttccgca
      361 gcggctgtaa agccccgccg tcatccccgt ttcagacgcg aagacatccc cgcgccggaa
      421 attgaccccg tgctgaaagc cttcgggcga cacattgccc ggagttttca ccgggggcgt
      481 ggtgtacaca tccccgccat gaaaaacacg gccttcgggc aggtgctccg cacactcgaa
      541 ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg aggggcggca acagacggaa
      601 ggagaacatt ttatgacatt atcagcaatc accatgaccg ccccggaagc cgccagcccg
      661 gtgcagatgt accgcgcgac ctactcaccg gatgataaca aacttcgcct gtatgccgtg
      721 tcacgcctcg acccggagac gtataaaaaa gtgcatgatg ccggtttccg ctgggcacca
      781 gaacaggcgc tgtttgtcgc gccagcctgg acaccgggcc gggaagacgt gcttctctca
      841 cttgccggag agattgagga tgaagacagt acgctcgctg aacgtcagga agcacgggcg
      901 gagcggttta ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa
      961 gtggaacgac tggccgcgat gatcccgccc ggtcagccga ttcttgtggg gcatcacagc
     1021 gaacgccgcg cccgtcgtga tgcgcagcgt attgaaaacg gcatgaaacg agccgtgatg
     1081 ctctttgcac gtgcggaata ctgggaagag cgggcgcggt cggcactgct tcacgcgaag
     1141 tataaagaac gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc
     1201 aaggctgaaa agaccatcgc gcagtcgcag aaatatctga cgatgtggcg ggctgaatcg
     1261 ctggatctga atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg
     1321 ctgaatacgt atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg atcgttatgg
     1381 tcggccctgg atgatgacat catcaccacg gagcaggccc gcgaaattgc gatccgctgt
     1441 catgaacggc agattcagca tcagcaacgc tgggttaacc actatcagaa ccgcctgatc
     1501 tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta cccggacaca ggattttgag
     1561 ccgggcggac aggttttcag ccggggagag tggctgacca ttatccgcgt gaacaaaagc
     1621 aacggggcgg tgagttcagt cacaacgccg aattacagtt tcctcgggta cagcggcacg
     1681 atgaaagtga cgcccgatcg catcacggac tacaaggcac catcggcaga agaggctgcc
     1741 gtcgccagtc aggccgcgaa gcgtccgccg gtagtcaact atccggggga aggtttccgg
     1801 gaaatgacaa aggcacagtg ggccgccctg ccccgggact gtaaggccgt gcgcagtgtg
     1861 gcagaagcag aagaccacgg ggcataccgc taccgccgca caatggacaa taatttccgc
     1921 ctggtgaatg tgtatatcac cgacatgaaa atcacggaaa tcccgcaaaa ataagactta
     1981 ccccccggag aaatccccgg gggatgacgg agacaatgac atgcacagtc agttaaggga
     2041 gcgtatcagg ctgatgcgcg caaggctgga caatgccagg ccagttatcg agattccggc
     2101 agaatttcag ctttttgtga cacctgcccc ggtctgcgat cgcctggtga cgctggcgga
     2161 aatcagcaac catgatcaca ttctggaacc ctctgccggc accggggcca tactgcgggc
     2221 gattcgggat accgcgccgg aggccatgtg tgatgcggtg gagattaaca gcgggctggt
     2281 ccggtatctg cgggaaaatt ttaacggtgt cagggtacag tgtggcgact tcatggaatg
     2341 gctgccggtg cagtattaca gccggatcat tatgaatccg ccgttcagtc acgggcagga
     2401 tatccggcat atcctgcggg ctttttccct gttgcgtccg gggggcgttc tggttgccgt
     2461 ctgtctcaac gggccacgcc agcaggagaa gctgttaccg ttttctgacg tccgcgagga
     2521 gctgccgcgc ggcacgtttg cttatacccg tgttccgacg atgattattc gtctgcgggc
     2581 ttgataacac agggcaggcg ggaggataaa tgcccgtctg tccgcgacgg gaaaagaccg
     2641 cgcgccggtg gcgctgaaag gggccgcgct cacgcgccgc ccctttcatt acgcggacgt
     2701 tctgatgggg gcttctgtct tttcagacct gtcatttgtc tgttactgat gatgttgttg
     2761 ttcctgctac tgctactgct actgctactg ctactgctct tctttttcct ggcgctctga
     2821 ttctgactac tgcagtgttg tcctgtctgc cgggcgcacc ttcggctccc ggctgcctgg
     2881 aggcatccgg tcgtcaggcc cgactccgac aggcagttgc tggcgcacct gccagtctcc
     2941 gccgaacccg ctggcgcggt ttcgggctgt cgctacggtc cgatgcgtga aaaccccggg
     3001 cttgcagcga ccgttccggt cgcgccgcct ctgtcgcgcc gtggcctgcg ctcccggcat
     3061 cccggcaaaa ggccgccggg atgtaaggcg caacgccgtt tcgcaaaccg cgcttcacgt
     3121 cgttgcggcg ggtccctgcc cgttgttctg tccggtgcca ttcacctgtt taagcccctg
     3181 tcgccggggg ctgatacagc cacaacctgc cgcactgact gtccagggta aatgaacagc
     3241 ccacaaaatt ttttacccaa aacccggtaa aaaattttgc gttctgccca ggacaggtgc
     3301 gtcaggccgt ggcagtgatg ccccttgcgg cgaaggttct caaacaggtg atggtgaata
     3361 ccggagggaa caaagagcag gtccccgcaa aacgtcaaaa ccgcggctcc cgaaactcaa
     3421 cagatgatgg gggctgaaaa catcaacaga aggagacaca tcatggcagt tcgtggcatt
     3481 aacaaggtca ttcttgtcgg tcgtctggga aaagatccgg aagtccgtta catcccaaac
     3541 gggggcgcag tggcaaacct gcaggtggcc acgtcagaaa gctggcgtga caaacagacg
     3601 ggggagatac gggagcagac agaatggcac cgcgtggtgc tgttcggcaa gctcgcggaa
     3661 gtggcaggtg aatatctgcg taagggcgcg caggtctaca tcgaggggca actccgtacc
     3721 cgtagctggg aagataacgg tatcacccgt tacgtcactg aaatccttgt taagaccacg
     3781 ggcaccatgc agatgctggg gagtgcacca cagcagaacg ctcaggcgca accggagcct
     3841 cagcagaatg ggcagccaca gagtgctgac gcgacgaaaa aagatggcgc gaaaacgaaa
     3901 ggccgtggac gtaaggccgc gcagcccgag cctcagcagc cgtctgaacc cgcgtatgac
     3961 ttcgatgacg acattccgtt ctgaacgact gactgtgaca accgccccgt ccagtgcggg
     4021 gcatcaccgg agagctgagg atgagcgaat atttcagaat atttcagggg ctgcctgacg
     4081 gctcctttac ccgcgaacag gctgaagccg ttgccgcaca gtaccggaat gtctttatcg
     4141 aggatgatca gggaacgcat tttcgcctgg ttgtccgtca ggatggcacg ttgatctggc
     4201 gctcctggaa ttttgaggac ggtgccgggt actggatgaa ccggtacatc agggatttcg
     4261 ggatccttaa gtaagagagg tgccggacgg ggatcacgtc cggcagacat taactacaca
     4321 ataaggggat agtcatgtca gtaacaaagt ctgaaccaaa atctacccgt aaagcaagcc
     4381 gtaaacctgc aaaaacgcag gaaactgtcc tgtctgccct gctggcgcag acggaggaag
     4441 tgagcgtgcc gctggtctca ctgattaaat caccgctgaa tgtgcgcacg gtgccgtatt
     4501 ctgcggagtc cgtcagtgaa ctggctgatt ccattaaggg agtcggcctg ctgcagaatc
     4561 tggttgttca tgccctgcca ggtgaccgct actgtgtcgc cgcaggcggt cgcagactgg
     4621 cagcactcaa catgctggca gagcgtggca tcattccggc tgactggcct gtccgcgtaa
     4681 aaattattcc gcaggagctg gcgactgccg catcaatgac tgaaaacggt catcgtcggg
     4741 atatgcaccc tgctgaacag atcgccggat tccgtgcaat ggcgcaggaa ggcaaaacac
     4801 ctgcacaaat cggtgatttg ctgggctatt caccccgcca tgtccagcga atgctgaaac
     4861 tggctgacct tgcgcctgtc atcctcgatg cgctggcaga agaccgcatt accaccgagc
     4921 actgtcaggc gctggcgctg gagaacgaca ccgcgcgtca ggtgcaggtg tttgaagccg
     4981 cctgtcagtc gggatggggc ggtaaaccgg aagtacagac cattcgtcgt ctggtgaccg
     5041 aaagtgaagt ggcggtggcg gggaacagta aataccgctt cgtgggggct gatgctttct
     5101 cacctgacga actgcgtact gatttgttca gcgatgacgg ggacggttat gtcgaccgcg
     5161 tggcgctcga tgctgccctg ctggaaaaac tccaggctgt cgctgaacac cttcgggaag
     5221 ccgaaggctg ggaatggtgc gccggacgca tggagcctgt cggtgagtgc cgtgaggatg
     5281 ccggaacata ccgctgtctg ccgcaaccgg aagcggtgct gacggaggca gaagaagaac
     5341 gcctgaacga actgatggca cgttacgacg cgctggaaaa ccagtgtgag gaatccgacc
     5401 tgctggaagc agaaatgaag ctgatgcgct gcatggcgaa ggtcagagcg tggacgccgg
     5461 agatgcgtgc cggaagtggc gtggtggtgt cctggcgtta cggtaatgtg tgtgtccagc
     5521 gtggtgtgca gttgcgcagc gaggatgacg cggctgacga cgctgaccgc acggaacagg
     5581 tgcaggagaa agcatcagtg gaggaaatca gtctgccgtt gctgacgaaa atgtcttccg
     5641 aacgcacgct ggcagtccag gcggcactca tgcagcaacc ggacaaatct ctggcactgc
     5701 tggcatggac gctctgcctg aatgtgtttg gcagcggagc gtacagtaaa ccagcacaaa
     5761 tcagcctgga atgtgaacat tattcgctga ccagcgatgc gccatcgggg aaggaaggtg
     5821 ccgcattcat ggcgatgatg gcagaaaaag cccgtcttgc ggccctgtta ccggagggat
     5881 ggtcacggga catgacgacg ttcctttcac tcagtcagga ggtgctgtta tccctgctca
     5941 gtttctgcac cgcgtgcagc atccacggcg ttcagacccg cgagtgtggt cacacgtcac
     6001 gcagcccgct ggacacgctg gagagcgcca tcggttttca catgcgcgac tggtggcagc
     6061 cgacaaaagc aaacttcttc ggacacctga aaaagccgca gattatcgca gccctgaatg
     6121 aggcaggact gtccggtgcc gcacgggacg cggagaagat gaagaaaggc gatgcggctg
     6181 aacatgcaga gttccatatg aaagacaacc gctgggtgcc tggctggatg tgtacaccac
     6241 gtccacagat ggatgccact gaacacacca ctaacctggc tgatgccgcc tgatgaacaa
     6301 ccacaccgcc ccgccggaga cggggcggca gcaagggaga taccgtgatg aaaactgaac
     6361 tgaccctgaa tgtattacag accatgaacg cacaggaata tgaagatatc cgggctgccg
     6421 gaagtgatga acgccgtgag ctgactcacg ccgtgatgcg ggagctggat gcgccggata
     6481 actggacgat gaacggcgag tacggcagcg agtttggcgg ctttttcccc gtccaggtcc
     6541 gtttcacgcc tgcccacgaa cgtttttacc tggccttatg ttcaccgggc gatgtctctc
     6601 aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg gttcaggtac
     6661 agcggcggtt tgcaccggaa gccgtcagcc attcactggc actggcggcg tcactggacg
     6721 cgcaggggta cagcgtcagt gacatcatcc atatcctgat ggcggaagga ggtcaggcat
     6781 gagcgcacgt tcacaggcac tggttcctct cagcacagag caacaggccg catggcgggc
     6841 ggtggcggag accgaaaaac gccgccatca gggtaacacg ctggctgaat atccgtatgc
     6901 gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg acctgcgatt
     6961 tttcatgccg tccctgaccg cagaagaact gcacggcaac cgtcttcagt ggctgtatgc
     7021 catcgatgtg ctgattgaaa cacagggcga ggtctgcctc cttccccttc ctggtgatgc
     7081 agcagagcgg ttgtttccgt cggtgcggtt tcgtgtcagg gagcgcagtc gccataaatc
     7141 cgcgctggtc atgcagaagt acagccgcca gcaggcacgg gaggccgagc agaaagcccg
     7201 tgcgtaccag gcgctggtcg ctcaggctga aatcgaactg gcattccatt cgccggaaac
     7261 agtcggaagc tggtatgcac gctggagtga ccgggtggca gagcatgacc tggaaaccct
     7321 gttctggcag tggggtgagc gttttccgtc actggccggg atggaacgct ggcagtggca
     7381 ggatatgcca ttctggcagg tcatcgcaga ggctggtatg gcggcaaggg aggccggtca
     7441 tgccgtccgt gagatggagc gatggatggt gccgaacaaa ctccgggagg cggcgtgaag
     7501 tggcgttact cactgcgctg gaaactgcct tatccctgtc ctggcgagca tgaactggtt
     7561 tcagaagtgg tggaggcggg gcagcctgca ccggcatccg ttatgtcacg ctgggttgcc
     7621 ggagcagggt atgcggtctg tctggatttt atttctgacc gcccggtgcg ccgctggagc
     7681 gaagaacgta aggctgcggt acgacggcgg aatctggaga aacgtatcaa caggcatgca
     7741 ccactgtttg cggatgaact gatagcccgt gaactggctg aacgaccgga ttattttcag
     7801 ggaaagtgag tgtggtcagt gcgcaggtat atgggctatg atgtgcccgg cgcttgaggc
     7861 tttctgcctc atgacgtgaa ggtggtttgt taccgtgtcg tgtggcagaa ggacaaaagc
     7921 cccgtagtta atttttcatt aacccacgag gcctctgcat gtctagtcca cgtcagtatg
     7981 gcctcttacc gtgcaaattg caaggaggag taaaccatga aactaccggg aaacgccctt
     8041 atctggtgcg tattaatcgt gtgctgcacg ttgttgatat tcacactcct gacccggaat
     8101 cgtctgtgcg aagtccggct gaaggacgga tacagggaag ttacggcaac aatggcttac
     8161 gaatccggcg gtaagtagca acctggaggc gggcgcaggc ccgccttttc agggctgatg
     8221 ctggtctgac tgcactgaag cgcctttata aaggggctgc tggttcgccg gtggcccctt
     8281 tctccttgct ggcattgtag cagtatgaac aaccagactg ccagaaaggc cacttccctc
     8341 cgggaacggg aacgggaatg aacgtgcgcc tgcggcgcac gttcttttcc cccgccttct
     8401 ctgtttatgg ccagttgtca gaaatgacat tacctttccc gtgattattc tctttttctt
     8461 cctgctctga ttctgactac tgcagtgttg tcctgtctgc cgggcgcacc ttcggctccc
     8521 ggctgc
//
LOCUS       DAZFFI010000162         8228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFFI010000162 DAZFFI010000000
VERSION     DAZFFI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.164"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /locus_tag="ACHJ12_004521"
     CDS             <1..284
                     /locus_tag="ACHJ12_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO4992211.1"
                     /translation="PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISE
                     SGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            284..5554
                     /gene="traI"
                     /locus_tag="ACHJ12_004522"
     CDS             284..5554
                     /gene="traI"
                     /locus_tag="ACHJ12_004522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO4992212.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYMDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDSREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGVSKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVFEPRADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5574..6320
                     /gene="traX"
                     /locus_tag="ACHJ12_004523"
     CDS             5574..6320
                     /gene="traX"
                     /locus_tag="ACHJ12_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO4992213.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRYEHIRQASVNRLWGWAVVAQF
                     GYFLAGYPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGAVG
                     IILLMLCHRIYRTTHLSVRSMLALCLFPVVALVNLQDGVTAAVSGVLVTYMVVRMVSG
                     IRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            6402..6770
                     /locus_tag="ACHJ12_004524"
     CDS             6402..6770
                     /locus_tag="ACHJ12_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFO4992214.1"
                     /translation="MKKILLVVAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGMT
                     EGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEVD
                     ISDEGNRKATVDVLTLERLR"
     gene            6806..7363
                     /locus_tag="ACHJ12_004525"
     CDS             6806..7363
                     /locus_tag="ACHJ12_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO4992215.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELWAILAE"
     gene            7517..7720
                     /locus_tag="ACHJ12_004526"
     CDS             7517..7720
                     /locus_tag="ACHJ12_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992216.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
BASE COUNT         1928 a         1995 c         2704 g         1601 t
ORIGIN      
        1 agccggtgtc tcctgccatc aacgataaga agtcagatgc aggtgtgaat gttccggcag
       61 gggggatcga gcaggagctg aaaatgaaac cggaagaaga gatggaacag caactgccac
      121 ccgggatcag tgaatccggt gaagtggtgg atatggccgt ttatgaggca tggcaacggg
      181 agcagaatcc ggatatccag cagcagatgc agcgccgtga agaggtgaat atcaacgtgc
      241 accgggagcg cggggaggat gttgagccgg gagatgattt ctgatgttga gtttcagcgt
      301 tgttaaatcg gcaggcagtg ccggaaacta ttacaccgac aaggataatt actatgtgct
      361 gggtagcatg ggggaacgct gggcgggcaa gggggctgaa cagcttgggc tgcagggcag
      421 tgttgataag gatgtcttta cccgtcttct ggaggggaag ctgccggacg gggcggattt
      481 aagccgtatg caggatggca gtaataagca tcgtcccggc tatgacctga ccttctccgc
      541 ccccaaaagt gtctccatga tggccatgtt aggtggcgat aagcgcctga ttgatgcaca
      601 taatcaggcc gtggattttg ctgtccgtca ggtggaggcg ctggcttcca cacgggtgat
      661 gacggacgga cagtcagaaa cggtactgac cggtaatctg gtgatggcac tctttaacca
      721 cgacaccagt cgcgatcagg acccacagtt acacacgcat gcggtggtgg ctaatgtcac
      781 gcagcataac ggcgaatgga agacactgag cagtgataaa gtggggaaaa ccggtttttc
      841 cgagaacgtg ctcgcgaacc gtattgcgtt cgggaaaata tatcagagtg agttacgtca
      901 gcgcgttgag gcgctgggct atgaaactga agtggtgggt aagcacggta tgtgggagat
      961 gccgggcgta ccggtggagg ccttttccgg acgctcacag gctatccggg aggccgtcgg
     1021 ggaagacgcc tcgctgaaat cccgggatgt ggcggcactg gatacgcgaa aatccaaaca
     1081 gcacgtcgat cctgaagtca gaatggctga atggatgcag acgctgaagg aaaccgggtt
     1141 cgacatccgg gcgtatcgtg acgcggcaga ccaacgggcg gagacccgca cgcagacacc
     1201 cggacccgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg ccattgccgg
     1261 attaagtgaa cgaaaagtgc agttcacgta catggacgtg ctggccagga cggtcggcat
     1321 actgccgccg gaagccggtg tgattgagcg ggcgcgtgcc ggtatcgatg aggccatcag
     1381 ccgtgagcag cttatccccc tcgaccgtga gaaggggctg ttcacgtccg ggattcatgt
     1441 gctcgatgag ctgtccgtcc gggcactcag tcgtgacatc atgaaacaga accgggtgac
     1501 tgtacatccg gagaaaagtg ttccccggac ggccggttac agcgatgcgg tcagtgttct
     1561 ggcacaggat cgcccgtcgc tggccattgt gtccgggcag ggcggtgcag ccgggcagcg
     1621 tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg tgcagattat
     1681 tgccgctgac cgtcgctcac agatgaacct gaagcaggat gaacggctgt ccggtgaact
     1741 gataaccgga cgtcgtcagc tgctggaagg catggccttc acgccgggca gtaccgttat
     1801 cgtcgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg acggtgccgc
     1861 acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta caggcagtgc
     1921 gctgatggcc atgaaggatg ccggggtgaa catatatcgc tggcaggggg gagagcagcg
     1981 accggcaacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc tggccggaga
     2041 ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg gagtacggga
     2101 acaggccata ctgacacagg ccattcgcag tgaactgaaa acgcagggcg tactcgggca
     2161 gagggaggtg accatgacgg tgctgtcacc ggtctggctg gacagccgga gccgttatct
     2221 gcgggatatg taccgcccgg gaatggtgat ggagcagtgg aacccggaga cacgcagtca
     2281 tgaccgctat gttatcgacc gggtgacagc gcagagtcac agcctgaccc tgcgggatgc
     2341 gcagggcgaa acgcaggtgg tgcgtatatc ctccctggac agcagctggt cgctgttccg
     2401 gccggaaaaa atgccggtgg cagacggcga gcgactgagg gtgacaggga aaattcccgg
     2461 actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag atgcgatgac
     2521 ggttgttgtg ccggggcggg ctgaaccggc caccctgcct gtggctgatt caccgttcac
     2581 ggcactgaag ctggagaatg gctgggtgga aacgcccggg cattccgtca gtgacagtgc
     2641 gaaggttttt gcctccgtca cacagatggc aatggacaac gccaccctga acggtctggc
     2701 ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta ctgcggaaaa
     2761 actcgcccgc catccgtcct ttacggtggt ttctgagcag ataaaggcgc gtgccggtga
     2821 gacattgctg gaaagtgcca tcagtcatca gaagtccgca cttcacacgc cggcacagca
     2881 ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca gcatggtgga
     2941 cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc agttttactg agctgggagg
     3001 ggaaatcaat gcgcagataa aacgcggtga tttactgtat gtggatgtgg caaaaggcta
     3061 tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca ttcttcgcca
     3121 tattctcgaa ggtaaggagg cggtcacgcc gctgatggag agggtacccg gcgaactcat
     3181 ggagacactg acgtcggggc agcgtgccgc cacccgcatg atactggaaa cgtccgaccg
     3241 tttcacggtg gtgcagggtt atgccggcgt gggtaagacc acacagttcc gggcggtgat
     3301 gtcagccgtg aagatgctgc cggagagtga gcgtccccgt gtcgtggggc tggggcccac
     3361 gcaccgtgcg gtcggggaga tgcgcagcgc cggcgtggat gcgcagacac tggcgtcttt
     3421 tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca gcaacacgct
     3481 gttcctgctc gatgagagct ctatggtggg caataccgac atggcacggg catacgccct
     3541 gattgcggcc ggtggtggtc gtgccgtggc cagcggtgac acggaccagc tgcaggccat
     3601 cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg tggccatcat
     3661 gaaagagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc tgattaaccg
     3721 ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa ccgtctcagg tgccacgtct
     3781 ggagggtgca tgggcaccgg agcactccgt gacggagttc agtcacagcc aggaagcgaa
     3841 actggcagaa gcgcagcaga aggcgatgct gaaaggcgag gctttcccgg atgttcccat
     3901 gacactgtat gaagccattg tccgcgacta taccggcagg acaccggaag tacgggagca
     3961 gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca tgattcatga
     4021 ttcacgggaa aaggccggtg agctggggaa agagcaggtc atggtgcctg tcctgaatac
     4081 agcgaatata cgcgacgggg aactgcgtcg tctctccacc tgggagaata acccggatgc
     4141 ccttgccctg gtggataatg tgtatcaccg gattgccggt gtcagtaagg atgacgggct
     4201 gataaccctg caggatgcgg aaggtaacac gcggctgatt tcgccccggg aggcagtggc
     4261 tgaaggcgtc acactgtaca ccccggacac catccgggtg ggaaccggtg accggatgcg
     4321 cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga ctgtgacagc
     4381 ggtttccggt gacagtgtca cgctgtcgga cggacagcag acccgggtga ttcgccccgg
     4441 ccaggagcag gcagagcaac atattgacct ggcctatgcc atcaccgctc acggtgcgca
     4501 gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc ggaaacagat
     4561 ggccggcttt gagtcagcct atgtggccct gtcgcgtatg aagcagcatg tgcaggtgta
     4621 caccgataac cgtcagggct ggacggatgc catcagcaaa gccgtacaga aaggaacggc
     4681 ccacgatgtg tttgagccaa gggctgaccg ggaggtcatg aatgcagagc ggctgttcag
     4741 tacggcgcgg gagctgcggg atgtggcagc tggtcgtgct gttctccgtc aggcagggct
     4801 gtccggggga gacagtcctg cacggtttat tgcaccgggg cgcaaatatc cgcagccgta
     4861 tgtggccctg ccggcttttg accgtaacgg caagtccgcc ggtatctggc tgaacccact
     4921 gaccacggat gacggaaacg ggctgcgggg attcagtggt gaaggccggg tgaagggcag
     4981 tgaagatgca cagtttgtgg ccctgcaggg cagccgtaac ggcgagagcc tgctggcgga
     5041 taatatgcag gatggtgtcc ggattgcccg tgataacccc gacagcggtg tggtggtgag
     5101 aatcgccggt gaaggtcgtc cgtggaatcc cggtgccatc accggtggtc gcgtgtgggg
     5161 ggatatcccg gacaacagcg tccagccggg ggccggaaac ggtgagccgg tcacggcaga
     5221 ggtgctggca cagcggcagg ctgaagaggc catccgtcgt gaaacggaac gccgtgcgga
     5281 tgagattgtc agaaaaatgg cagaggaaaa acctgaccag ccggatggta aaacagagca
     5341 ggctgtcagg gctattgccg ggcaggagca ggacagggct tcgtcatcag aacgggaaac
     5401 tgcgctgccg gagagtgtgc tgcgtgagcc tcaacgggtg caggacgctg tccgggaggt
     5461 tgcccgggaa aatctgctgc aggagcgact tcagcagatg gagcgggata tggtccgtga
     5521 cctgcagaaa gagaaaaccc tgggcggaga ctgatacagg aagacaaagg ctgatgacaa
     5581 cggataacac gaacacgaca cgtaacgatt cactggctgc ccggaccggt acctggctgc
     5641 agtcgttgct ggtctggtca cccggacagc gggatatcat caaaacggtg gcactggtgt
     5701 tgatggtgct ggaccatgca aaccgcattc tgcatctgga gcagacgtgg atgtttctgg
     5761 ccgggcgggg cgcgtttcca ctgtttgcgc tggtctgggg actgaatctg tcacggtatg
     5821 agcatatccg tcaggcgtcc gtaaaccgcc tgtggggatg ggctgttgtg gcgcagttcg
     5881 ggtactttct ggcgggatat ccgtggtatg agggcaatat tctgtttgcc tttgcggtgg
     5941 cagcacagat gctgaaatgg tgtgaatatc actcgctggt atggtcagtc agtgcactta
     6001 cacttctggt tctctggttc ccgttatctt ccaccagtta cggtgctgtc gggatcatat
     6061 tactgatgct ctgtcacagg atatacagga cgacgcatct ttctgtacgg agcatgctgg
     6121 cgctctgtct ttttccggtc gttgctctcg ttaacctgca ggacggcgtg actgccgctg
     6181 tcagtggtgt gttagtgaca tacatggtcg tcaggatggt atccggtatc cggtggcctg
     6241 ctgcccgttt ctggccggca gactttttcc cggtatttta tgtctgtcat ctcgctgtgc
     6301 tgggagtgat ggccctgtag caggaagcgc agaaagcgga tgaactgtga aagtcttttt
     6361 gtttaaatca gaacgcattc aggaaaaaac aggagcagat gatgaaaaaa atattactgg
     6421 tggtagccat tacgggaatg ttcgttcagg gggtatgggc aaaatcagat cagcaaatcc
     6481 gggatgaggc taaaaataat gtgatcactg tttctcaggt gagtggcatg acggagggga
     6541 ccggtgtgac gctgaatggt cagatagtga agcaactggc agccgaagag gatcattttg
     6601 aactcagtga tgcatcagga agtattgtca ttgatgtcga tgatgaagta tggcgcccgc
     6661 tggccctgaa agccggagat aaagttcagg ttgtgggaga agtggatatt tctgatgagg
     6721 gaaaccggaa agccacggtg gatgtactga ctcttgagcg tcttcgctga tatgaacaac
     6781 ggataccggt gagggcaggt gagttatgac agagcagaaa cggccagtac tgacactgaa
     6841 acgaagaacc gacgtggaaa caccgacccg gagccggaaa accatcatca atgtcaccac
     6901 gccaccaaaa tggaaggtga aaaagcagaa actggcggag aaggctgccc gggaagcaga
     6961 gctggcggcg aagaaagcac aggccagaca ggcgctgtcc atttatctga cgctgccctc
     7021 gctggatgag gcggtgaaca ctctgaaacc ctggtggccg ggattgtttg acggtaacac
     7081 gccccggctt cttgcctgcg gtatccggga ggttttactg gatgaggtgt ctcagcgaaa
     7141 tatcccgctc tctcataaaa aactcagccg ggcgctgaaa gccatcaccc gttcggaaag
     7201 ttatctgtgc gcaatgaaag ccggcgcctg ccgctatgat acggaaggct atgtgacaga
     7261 acatatcaca caggaagaag aacagtatgc gcaggcgcgc ctggaaaagg tgcgcagaca
     7321 gaacaggatc aaggatgagc tgtgggcgat cctggcggaa taataaaaac gccctctgta
     7381 tgacagaggg caacagcgaa aaaatcggga aaacagacat ccagagtttt gatggagaaa
     7441 aacacccaac cgttcaggga tgtttcagaa tgtttctgac ataatccggg agccgggtca
     7501 agagggggga taaaaaatgg ctgtatccag tggattatct gagcacagcg gtatttatcg
     7561 ctatcagcgc gtggggcgtc atgccgtccg tcaggagatt cgtggttttt tcagggaagc
     7621 cgctgaggcc tggcagaatg cggcccggct ggcaccctgt gaagcatggc gggattttgc
     7681 cctgaagaga gcatctgcat gccggtgtaa gtgccgataa aaataacccc cgttgcggag
     7741 cgccggggga tcaggcttat atggcagatc gttattatta tagtggtgta agaaggagtt
     7801 gccctgtctt tcaaacggac atcacagagt taacatattt cacatcccgg gtaagccagc
     7861 agggataaaa aatgtgatga atttcaagaa gcaggaggtg acagtggcat gcataacagc
     7921 agggttatcc ggtgaatctg acagacagtc gggctgtatt cgcggcagga taaagaaaaa
     7981 ccgggagatg actctcccgg tccgtcttat cgctaatccc ctgagcaggg catgtatgac
     8041 ttatctatga ccgttatatt cagcgccaca tccggtagcc tgcgatataa cacagttatc
     8101 ctatcagcaa gagcaggaat gtttaatact caggggtgaa aaatataatc tgtatcacaa
     8161 agtagtatgt agcaggggga agccgatagg gtacgaacgg ggaacgggaa tacatgacct
     8221 tgcgaaca
//
LOCUS       DAZFFI010000163         8178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFFI010000163 DAZFFI010000000
VERSION     DAZFFI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.165"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            34..531
                     /gene="ftnA"
                     /locus_tag="ACHJ12_004527"
     CDS             34..531
                     /gene="ftnA"
                     /locus_tag="ACHJ12_004527"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4992217.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(569..808)
                     /gene="yecH"
                     /locus_tag="ACHJ12_004528"
     CDS             complement(569..808)
                     /gene="yecH"
                     /locus_tag="ACHJ12_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4992218.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            999..2210
                     /gene="tyrP"
                     /locus_tag="ACHJ12_004529"
     CDS             999..2210
                     /gene="tyrP"
                     /locus_tag="ACHJ12_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4992219.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2272..2937)
                     /gene="yecA"
                     /locus_tag="ACHJ12_004530"
     CDS             complement(2272..2937)
                     /gene="yecA"
                     /locus_tag="ACHJ12_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4992220.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3133..3219)
                     /locus_tag="ACHJ12_004531"
     tRNA            complement(3133..3219)
                     /locus_tag="ACHJ12_004531"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3183..3185),aa:Leu,seq:taa)
     gene            complement(3232..3305)
                     /locus_tag="ACHJ12_004532"
     tRNA            complement(3232..3305)
                     /locus_tag="ACHJ12_004532"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3271..3273),aa:Cys,seq:gca)
     gene            complement(3359..3434)
                     /locus_tag="ACHJ12_004533"
     tRNA            complement(3359..3434)
                     /locus_tag="ACHJ12_004533"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3399..3401),aa:Gly,seq:gcc)
     gene            complement(3586..4134)
                     /gene="pgsA"
                     /locus_tag="ACHJ12_004534"
     CDS             complement(3586..4134)
                     /gene="pgsA"
                     /locus_tag="ACHJ12_004534"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4992221.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(4191..6023)
                     /gene="uvrC"
                     /locus_tag="ACHJ12_004535"
     CDS             complement(4191..6023)
                     /gene="uvrC"
                     /locus_tag="ACHJ12_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4992222.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6020..6676)
                     /gene="uvrY"
                     /locus_tag="ACHJ12_004536"
     CDS             complement(6020..6676)
                     /gene="uvrY"
                     /locus_tag="ACHJ12_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4992223.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6818..6889
                     /locus_tag="ACHJ12_004537"
                     /pseudo
     CDS             6818..6889
                     /locus_tag="ACHJ12_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6972..7148
                     /gene="yecU"
                     /locus_tag="ACHJ12_004538"
     CDS             6972..7148
                     /gene="yecU"
                     /locus_tag="ACHJ12_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4992224.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7135..7359
                     /gene="yecF"
                     /locus_tag="ACHJ12_004539"
     CDS             7135..7359
                     /gene="yecF"
                     /locus_tag="ACHJ12_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4992225.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHJ12_004540"
     CDS             complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHJ12_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4992226.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2028 a         2099 c         1916 g         2135 t
ORIGIN      
        1 cagcaacaaa tataaccttt gtggagcact atcatgctga aaccagaaat gattgaaaaa
       61 cttaatgagc agatgaacct ggaactgtac tcttcactgc tttatcagca aatgagcgcc
      121 tggtgcagct atcatacctt cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa
      181 gagatgacgc atatgcagcg tctgtttgat tacctgactg ataccggcaa tttaccgcgt
      241 attaataccg ttgaatctcc gtttgctgaa tattcctcac ttgatgaatt attccaggaa
      301 acctataaac acgaacaatt aatcacccag aaaattaacg aactggctca tgctgcaatg
      361 accaatcagg actacccaac atttaatttc ctgcaatggt atgtttctga gcagcatgaa
      421 gaagagaaac tgttcaaatc gattattgat aaattaagcc tggcaggcaa aagcggcgaa
      481 ggtctgtatt ttatcgacaa agaactctct accctcgaca cacaaaacta atgctctccg
      541 gcggcaggtt tactgccgcc gtttcaaatc agtgacggca aatcttactc tgatcggtcg
      601 aaaaaccgtc tttcgaaggt ataaatttgc cttttgctgc cagaaacgct accagctctc
      661 ccgctgtcat cccttctgcc gagcaggtgt gaaaacgtgc ctgttcacca aaacgcgctt
      721 taatcgcagc ttccagactg gcatgcgtat attgctcgcc tgattcaatc atcatattta
      781 acacttcatg accgtgaata gagtccatcg tccctcctca aaaaaagcct agcgtagcga
      841 ttgccgctta tgaagacttt gcgccagcgc aggactgaat gctttttatt gtacatttat
      901 atttacacca tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
      961 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     1021 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     1081 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     1141 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     1201 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     1261 tgttcttaat gtatgctctg actgcggcat acatcagcgg tgccggtgaa ttgttggcct
     1321 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     1381 cttttgttgc cggtggcgtg gtttgtgtcg gaacttcact ggtcgattta tttaaccgtt
     1441 ttctgttcag cgccaagatt atttttctgg tggtaatgct ggtactactg ctgccgcata
     1501 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggctctg tctgcaatcc
     1561 cggtgatttt tacctcattt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     1621 atggcaacgt tcgtaagcta cgctgggtgt ttataaccgg tagtgcgatc cccctggtgg
     1681 catatatttt ctggcaggtg gcaacgcttg gcagcattga ttcaacaacc tttatgggat
     1741 tgctggctaa tcatgccgga ttaaacgggc tgttacaggc attacgcgaa atggtggcct
     1801 ctccgcatgt tgagctggca gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     1861 tcggcgttgc gttaggctta tttgattatc tggctgattt atttcagcgt tcaaataccg
     1921 ttggtggacg gttgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     1981 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     2041 cattgattat cccttcgctg ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     2101 accgggtgaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     2161 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     2221 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatttgacct cttaatgcag
     2281 gcagcactgc ttaaatttct taccactacc gcacgggcaa ggatcgttac gccccggttt
     2341 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtacgc
     2401 atgtagatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     2461 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     2521 tggttttaac gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     2581 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
     2641 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
     2701 agccagattc ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca catagtcagc
     2761 cccaccccac acggcaacca gccactgttc cggttcaatc tcttgcggag aactcaacac
     2821 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
     2881 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
     2941 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
     3001 tactttttga gtggctttgc tggtgattaa aaattaagga gggtgtaacg acaagttgca
     3061 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattgcttt gattttattc
     3121 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
     3181 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
     3241 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
     3301 gcgccaaatt cttcaggcct ttcagccaga catccgctga cgccgatgtc ttttaaactg
     3361 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
     3421 tgagctattc ccgcattcat caagcaatca gttaatcact tgattttatt atcgtctggc
     3481 aatcagtgcc gccgttcgat gcgttgcatt ctacttacct ggcgcgatga gtcaacgata
     3541 tttttcacca cttttgattg tttgctgaaa attacgccga aacgatcact gttcaagcaa
     3601 atctgcacgc gcagcgctca aatattgcaa cattgaccac agagtcagta ccgcagccac
     3661 aaagaaaagt gcaataccgg cgtactcaac ccaaatgttc ggacgccaca gcagccaggc
     3721 caacgccacc atctgggcag tggttttcac tttcccaatc caggagacag ccacgctact
     3781 gcgtttaccc aactccgcca tccattcgcg tagcgcagaa ataataattt cacgggcgat
     3841 catcgttgcc gccggtaagg tcacccacca gctgtgataa tgctcggtta ccagcaccat
     3901 ggcgatagcc acgagaactt tatctgcgac agggtcaagg aaagcaccaa accgggtact
     3961 ctggttccag cggcgtgcca gaaaaccatc gaaccagtca gtcaccgccg cgacgcagaa
     4021 aatgagcgcg gcggcaaacg gcgaccaggt gacaggcaga taaaagacca atacaaagaa
     4081 tgggataagg atgacacgga acagtgtaag caacgtaggg atattaaatt gcataatgac
     4141 gggtaactat ctgttgtcag taagattacc cctatgttgc tacagagaca tcaatgtttc
     4201 aacgaccaga agatcttttc tgccagacct tgcgaaatac ccggcacttt tgcaatttcc
     4261 tcgacgctgg cgttacgtaa accttgcaaa ccgcccatat atttcaacaa catttgccgg
     4321 cgttttggcc cgacgccttc aatggtttcc agggaactgg tatttttgac cttcgcccgt
     4381 tttttacggt gcccgccaat cgcgtgatca tgtgattcat cgcgaatatg ctggataaca
     4441 tgcagcgcgg gagaatctgg cggcaaactg aatccctcac cttccggctc aaagaacagc
     4501 gtttccagcc cagccttacg atctgctcct ttggcaacgc caagtagcag cggatgattt
     4561 ttatcccatg agacatccag ttcggcgaag acatttttcg cctgcgcaag ctggcctttg
     4621 ccgccgtcga taaggatcac atccgggatc ttactgtcgt caatggcttt accataacgc
     4681 cgacgcagca cctgattcat cgccgcataa tcatcgcccg gcgtgatgcc tgtaatgtta
     4741 tagcgccgat actccgcacg cagcgggccg ttagcatcaa acaccacaca ggaagcgacg
     4801 gtttgttcgc ccatggtatg gctgatgtca aagcactcca tccgcttcac ttccggcaat
     4861 ttcaacacgc tggcaagcgc ggtcagtcgc tggtgaacgg tagattgctg cgaaagtttg
     4921 ctggttaagg ccgtcgccgc attggtgcgc gcgagtttca gataacgcgc cctatcgccg
     4981 cgaggtttgg tttgaacatt aatcttgcgt cccgccagtt ctgaaaggga atcggcgagc
     5041 agcgttttat cgctaagatt aaaatcgagc aggatctcac ccggtaaggt gcgcatctgg
     5101 ctgccttgta aatagaactg gcctacgaag gtttctacca cctcgctcag ttccgtaccg
     5161 ccaggcactt tcgggaaata gctgcggctg ccgagcactt tgccctgacg aatgaacaat
     5221 acgtggacac aagccatgcc cgcatcgaac gccacaccaa taacgtcgag gtcgtcgccg
     5281 gtattggaaa cgaattgttt ttcggtgacg cgtcgcaccg cctgaatttg gtcgcgaata
     5341 cgtgcagctt cttcaaactc cagattctgg ctggcagttt ccatacgact aatgagttgc
     5401 gtaagcacct gatcatcttt gccagacaaa aacaggcgca catactcgac ctgctgagcg
     5461 tattcttctt cactcaccag tccttcaacg cacggtccca gacagcgccc tatctggtat
     5521 tgcagacacg gacgcgagcg attgcgataa acactatttt cgcactggcg aatggggaaa
     5581 atcttttgca gtagcgccag agtttcacgt acggcatagc cattcgggaa cgggccgaaa
     5641 tattcacctt tggcatgctt cgcaccacga tgcatcgcca gacgcgggtg ggtatcacca
     5701 ctcaggaaga taaaaggata tgatttatca tcgcgtagca aaacgttgta acgcggctga
     5761 tagagtttga tgtagttgtg ttccagcaac agcgcttcgg tttctgtgtg agtaaccgtt
     5821 acatcaattt gctggatctg ggcgaccagc gcttcggttt tgcgcgaagc gaggttgcta
     5881 cggaaatagc tggaaagccg ttttttcagg tctttcgctt tgccgacata gataaccgta
     5941 ccaccagcat cgtacatgcg ataaacgcct ggctggctgg ttacggtttt taaaaacgct
     6001 tttgcgtcaa actgatcact cactgacttg ataatgtctc cgcattacac agaccatggc
     6061 gaattgccag gtgagtcagc tcaacatcgc catgaatgtt tagtttactg aacatacgat
     6121 agcggtagct gttcaccgtt ttcggactga gattgagctg ttctgagatc tcattgacct
     6181 tctggccctt ggtgatcatc agcataatct gcaattcacg ttcagacaaa ctggcaaatg
     6241 ggctttctgt tttttctggt tcgatctggc ttaacgccat ttgttgagcg atgtcagaag
     6301 caatgtaacg ctgccctgaa tagacagaac gaatcgcact cacgacttcc tgcggagccg
     6361 cgcctttgct gaggtagccc gcagcaccgg cctgcatgac tttcgctggt aaagggtttt
     6421 ctgtatggac ggtaagcatg atgattttga catcagctgt ggaacgcgcg attttacgcg
     6481 tcgcctcaag accgccaatg cccggcatac tcatgtccat tagcaccacg tcaacggcat
     6541 ttgtccggca ccacttaacg gcgtcttcac cgcacgatgc ctcaccgacg acttttatac
     6601 cctttatatc ttccagaatg cgtcgtatcc ctgcgcgcac cagttcgtgg tcatcaacaa
     6661 gtagaacgtt gatcaaagga atatctccag aaatagggat aacgctactg atagttagtc
     6721 attcgtatat tagcggtttt tattgcaact ttgaaacgtt aaaaatggtg ggtattcgat
     6781 ttttctctcg tttttgtcaa ttcgattgtc cacagttatg gtcacgcccg tcaccaacgg
     6841 cttaccggat cgtttcacag taagccctac gatgaaatgt gacaaaaatt gactttattc
     6901 agcaaaaatg aaaatcagcc cgctgaatat gtaacattaa ttaacccgaa caatacgggc
     6961 aaaaaacatt catgataaaa atatttattg gtcattatat taacgtattt tatagcactg
     7021 ccgatatcac gctcaaaaaa caaccactgc tatttttagc aaagcttatg gtatactccg
     7081 ccgccttaac atttttcacc gcaaattttc attgcaacat gacgaggaaa ataaatgagt
     7141 acgcctgatt tttctactgc cgagaataat caagaactgg caaatgaagt ctcctgcctg
     7201 aaagcgatgc tgacgctgat gctgcaggcg atgggacaag ctgacgcggg ccgcgtgatg
     7261 ttaaagatgg aaaaacagct tgcgctgatc gaagacgaaa cccaggctgc agtattttcc
     7321 aaaacggtta agcaaattaa acaagcctac cgtcagtaat ataaaaccgg ctgagagcgt
     7381 gcctttcagc cggtttttgc atctggcacg cagaaagaaa agcgatcaaa ttaagccagt
     7441 agcggccgcg taacaggcca cctgggtctt atttggtgca ttgatttttt tctgcatgtt
     7501 tttctgatgg aaattgaccg tattctcaga gattgacaaa atcatcgcta tctctgctga
     7561 tgtcttccct tccgccgtcc atttcagaat ttctttttcg cgcttgctga aattcatctc
     7621 tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag
     7681 taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgag cgctgcaacg
     7741 ggaaaaggac aaaaagccca acgcccgatt tggcagcatt aaatactgag tgacaccgcg
     7801 gcgtaaacca tgtgcgcgcg cggcttccca taacggctgt gcttcgctga ataagtcatc
     7861 attccacatt aaatggccct gactaaagtt ttcagggttc agcaccggat caattgcgag
     7921 aaagtttttt gcctgataat aactaaccca cgcctcaggg taattggtgt agaaagccac
     7981 tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat cgtactccag
     8041 ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca tcctctgaaa
     8101 acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag taaaccgcaa
     8161 cgcccctgag agtgagta
//
LOCUS       DAZFFI010000164         8118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFFI010000164 DAZFFI010000000
VERSION     DAZFFI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.166"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..262
                     /locus_tag="ACHJ12_004541"
     CDS             <1..262
                     /locus_tag="ACHJ12_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992227.1"
                     /translation="DNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSR
                     HEMLPVNLTQETLFSSKSQDKHALFPIFTASWRAHRIMNKGV"
     gene            485..982
                     /gene="ubiC"
                     /locus_tag="ACHJ12_004542"
     CDS             485..982
                     /gene="ubiC"
                     /locus_tag="ACHJ12_004542"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4992228.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            995..1867
                     /gene="ubiA"
                     /locus_tag="ACHJ12_004543"
     CDS             995..1867
                     /gene="ubiA"
                     /locus_tag="ACHJ12_004543"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4992229.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2022..4445)
                     /gene="plsB"
                     /locus_tag="ACHJ12_004544"
     CDS             complement(2022..4445)
                     /gene="plsB"
                     /locus_tag="ACHJ12_004544"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4992230.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     NDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4616..4984
                     /gene="dgkA"
                     /locus_tag="ACHJ12_004545"
     CDS             4616..4984
                     /gene="dgkA"
                     /locus_tag="ACHJ12_004545"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4992231.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5094..5702
                     /gene="lexA"
                     /locus_tag="ACHJ12_004546"
     CDS             5094..5702
                     /gene="lexA"
                     /locus_tag="ACHJ12_004546"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4992232.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5775..7100
                     /gene="dinF"
                     /locus_tag="ACHJ12_004547"
     CDS             5775..7100
                     /gene="dinF"
                     /locus_tag="ACHJ12_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4992233.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7216..7425
                     /gene="yjbJ"
                     /locus_tag="ACHJ12_004548"
     CDS             7216..7425
                     /gene="yjbJ"
                     /locus_tag="ACHJ12_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4992234.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7467..7982)
                     /gene="zur"
                     /locus_tag="ACHJ12_004549"
     CDS             complement(7467..7982)
                     /gene="zur"
                     /locus_tag="ACHJ12_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4992235.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1818 a         1993 c         2233 g         2074 t
ORIGIN      
        1 cgataatata tttatcctgt tctcctctgt cgatgatggg cctgttatgg tggtaagctc
       61 ccagcgctta catgatatgt tgaatcctac aaaagatacc aattggaatt ccacgtatat
      121 ctacaaatcc agacatgaga tgttgcctgt taatcttact caggaaacac ttttcagctc
      181 caaatctcag gataaacatg cgcttttccc catttttact gcgagttggc gagctcatcg
      241 tataatgaat aagggtgttt aagtaaagga aaacatcacc gttcctggca tcctgggcgg
      301 tgatgcccct atggttgccc tctccagcgc gggcatcggc aaagcgtaag gttcaacagc
      361 gttttaccac ttcatgcgat tgttgcgttt ttgttgcgta ttagatcact taatttgctt
      421 tacatctccc gtaaacactt ttctgcgata caatgccttt acgttatgta acggagagtt
      481 cggcatgtca caccccgcgt taacgcaact gcgtgcgctg cgctatttta aagagatccc
      541 tgccctggaa ccgcaactgc tcgactggct gttgctggag gattccatga caaaacgttt
      601 tgaacagcag ggaaaaacgg taagcgtgac gatgatccgc gaagggtttg tcgagcagaa
      661 tgaaatcccc gaagaactgc cgctgctgcc gaaagagtct cgttactggt tacgtgaaat
      721 tttgttatgt gccgatggtg aaccgtggct tgccggtcgt accgtcgttc ctgtgtcaac
      781 gttaagcggg ccggagctgg cgttacaaaa attgggtaaa acgccgttag gacgctatct
      841 gttcacatca tcgacattaa cccgggactt tattgagata ggccgtgatg ccggactgtg
      901 ggggcgacgt tcccgcctgc gattaagcgg taaaccgctg ttgctaacag aactgttttt
      961 accggcgtca ccgttgtact aagaggaaaa aaatatggag tggagtctga cgcagaataa
     1021 gctgctggcg tttcatcgct taatgcgtac ggataagcca attggcgcgt tactgctgct
     1081 ctggccaaca ttatgggcgt tgtgggtggc gacaccgggc gttcctcagc tctggatcct
     1141 ggcggtattt gtcgcgggtg tctggctgat gcgcgctgct ggatgtgtgg tgaatgatta
     1201 tgctgaccga aagtttgatg gtcatgttaa gcgcacggcg aaccgaccac ttcccagcgg
     1261 cgcggtaaca gagaaagagg cgcgcgcgct gtttgtagtg ctggtactga tttcgttttt
     1321 actggtactg acgctgaata cgatgaccat tctgttgtcg attgccgctc tggcgttggc
     1381 gtgggtgtac ccgtttatga agcggtatac ccatctcccg caagtggtgc tgggcgcggc
     1441 gtttggctgg tcgattccaa tggcttttgc cgctgtgagt gagtcggtgc cattgagttg
     1501 ctggttaatg ttcctcgcca atattctctg ggcggtggct tacgacacgc agtatgcgat
     1561 ggttgaccgc gatgatgatg tgaagattgg cattaaatcc acggcaatcc tgttcggcca
     1621 atacgataaa ttgattattg gtattttgca gattggcgta ctggcactga tggcgatcat
     1681 cggtgagtta aatggcttag gctggggata ttactggtca attgtggtgg ctggcgcgct
     1741 gtttgtttat caacaaaaac tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt
     1801 tatgaataat aactatgttg gtctggtact gtttttagga ctggcaatga gttactggca
     1861 tttctgatga tgtaaaaaag ccggatgatc atccggcttt cttctgggtt gccttatgcg
     1921 cggcgcttct caggcctaca caacacatcg caatttattg aatttgcaga ttatggaagg
     1981 ccggataagg cgttttcgcc gcatccggca attctctctg attacccttc gccctgcgtc
     2041 gcactctcaa tcgtcaaacg cacgtctgat gtaatcaact ccgccagcaa ctgataaacc
     2101 ttcatcgttt ctgccggttc ggcgtcgccg ctatcgctga tatacccttc atcacgcagc
     2161 gtcagcacca gagaactgaa caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc
     2221 agcacggaga gacgttgcgc gacggtgcgg ctctctttct ccagcgtacc gcggttgatc
     2281 gacgggttgg cactcaacaa ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg
     2341 cctgcggcca gcagctgtag ggtgcgagaa tgcgccgggt tgatatgcaa ctcatcattt
     2401 tgcagggtaa tcagcccctg acgttgcatc tcatttgcca gcgcatcaat aacgtccggc
     2461 aactcgtcgc gatcccagcg caggaacagc tccgctttca gcattgggta aagcacattg
     2521 acgtgctcca tcaatacgtc gcgggagatg tggcgatgct gggtgacaat tgccgccatc
     2581 agcgaaggca gcaccaacat atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct
     2641 tgctcgcgtg gcagaatgat gatgtcgccg atagtgtctt tctcgacttc aaacttgttc
     2701 atttgcagcg cgtgatcgat aagctcgctg gcgctggctg aaggaacggt agagtccgtg
     2761 gagtaaggta cgttgcgcat cagatccagg tagcagttga gttgctcggt taactgctcg
     2821 cgggtgagtg agcgctgacg tgatgccaga agcgcagtac aacacaggtt catggcgttt
     2881 gccgcgcctg cgttgttaat gcgtaccatc agatcggcag caatattatt gaccgtcggc
     2941 gttaaccagg ccggacgcac cgcttcgatg ggatcgatag attcacgcca gtctggtacg
     3001 tgctggttaa ggtaggtcat caacggcatt ggttcaccga agttgacgta accctgaccg
     3061 agattacgca gcttgcttaa accgcgcagc atctgcggca ggctctcttt ctctttcgtc
     3121 gcgccgcgca gttctttggc gtaagtaccc acttccatga cgtgctcata accgatatag
     3181 atcggaatca gcgtaatcgg acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac
     3241 agcgtaccgg ttttcggatc cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag
     3301 tactcgacgg aataaccacg gctgaacagt tcgccgagat actcacggaa aacggtggaa
     3361 taaagtttat tgcctttaaa agtacggcga ataaagaacg cccccagacg gcggaaaatc
     3421 ggcccggccg gccagaagtt caggttgatc ccggcggcga tatgcggcgg caccagcccc
     3481 tggtggtaca gcacgtaaga aagtagcagg tagtccatgt gactgcggtg gcaaggcaca
     3541 tacaccagct catgaccgtc gtgggccagc tggcgaacgc gctcggcgtt atggacgttg
     3601 atgccctgat aaagtcggtt ccaggtgaag cccagaatgc ggtcagtcag gcggatcatc
     3661 tcgtaagaga aattcgccgc aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt
     3721 tcatgggaga tttttttgct gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag
     3781 gcgagcagct tattaaacag atcctgacga gcaggcagac gtgggcccac agcagccaga
     3841 cgttgacggg caaagtgcat acgcgccacg cgcgccagtt tctgtgcgat agttttatcc
     3901 gtgccgtgtt catccgccat acggcgcagc gaaactgacg gcgagaaacg cacaaaactg
     3961 tcgcgaccga gccacagtac ggcgaaaaat ttctgtacgc cgttaagcat acgcagcggc
     4021 gggtttactt cgcctttttc acgccccggc gcgcgaccaa acatcaccga tactggcacc
     4081 atctgcacat ccagatttgg gttgctgcgg tgcaaatcaa gatagtcgtg gaacagctta
     4141 atagactctt ctttcggcgt gtaataggtg aacacacgcg gcccgccgtg aatgaacaca
     4201 tagcgcggca gtagcgtgcc gtcgatttcc agcggctcta acgggtcagg caagtcatga
     4261 gccagacact gggcgcgcaa cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac
     4321 ataattggac gagaggtatc cagccccagt tccggggcag gatctgccgg aatagacttg
     4381 ctttttacca ggatgcttaa tggtaaattc agtaatttgt agtaaattcg tggccagccg
     4441 gacataaacg atgtaaagcc tctggttaat aatgcaaatg cgcggcaagg atagcagaaa
     4501 gtcatgggaa attctgtggt atccgctcat gtttcgcgcg gcgttacgca aacccgaatc
     4561 atcggattta acggtacact gatattgacg ctcataatgt aaaaaggttc tttcaatggc
     4621 caataatacc actggattca cccgaattat caaagctgct ggctattcct ggaaaggttt
     4681 acgcgctgca tggatcaacg aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc
     4741 ggtggtcatc gcctgctggc tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc
     4801 cgtgatgctg gtgatgattg tggaaatcct caatagcgcc atcgaagcag tggttgaccg
     4861 aattggctct gaataccatg agctttccgg acgcgcaaaa gatatgggat ccgccgcggt
     4921 gctgattgcc attatcgtcg ccgtgattac ctggtgcatc ctgttatggt cgcattttgg
     4981 ataacccttc cagatttcga taaatctctg gtttattgtg cagtttatgg ttccaaaatc
     5041 gccttttgct gtatatactc acagcataac tgtatataca cccagggggc ggaatgaaag
     5101 cgttaacggc caggcaacaa gaggtgtttg atctcatccg tgatcacatc agccagacag
     5161 gtatgccgcc gacgcgtgcg gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg
     5221 ctgaagaaca tctgaaggcg ctggcacgca aaggcgttat tgaaattgtt tccggcgcat
     5281 cacgcgggat tcgtctgttg caggaagagg aagaagggtt gccgctggta ggtcgtgtgg
     5341 ctgccggtga accgcttctg gcgcaacagc atattgaagg tcattatcag gtcgatcctt
     5401 ccttgttcaa gccgaatgct gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata
     5461 tcggcattat ggatggtgac ttgctggcag tgcataaaac tcaggatgta cgtaacggtc
     5521 aggtcgttgt cgcacgtatt gatgacgaag ttaccgttaa gcgcctgaaa aaacagggca
     5581 ataaagtcga actgttgcca gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc
     5641 agcagagctt caccattgaa gggctggcgg ttggggttat tcgcaacggc gactggctgt
     5701 aacatatctc tgagaccgcg atgccgccag gcgtcgcggt ttgtttttca tctctcttca
     5761 tcaggcttgt ctgcatggca ttcctcactt catctgataa agcactctgg catctcgcct
     5821 tacccatgat tttctccaat atcaccgttc cgttgctggg actggtcgat acggcggtaa
     5881 ttggtcatct tgatagcccg gtttatttgg gcggcgtggc ggttggtgca acggcgacca
     5941 gctttctctt tatgctgttg ctgtttttac gcatgagcac caccgggctg actgcgcagg
     6001 cttatggtgc caaaaatcct caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg
     6061 cgttgggggc tggggcgtta attgcgctgc tgcgtacacc gattatcgat ctggcgctgc
     6121 atattgttgg cggtagtgaa gcggtcctgg aacaggcgcg gcgctttctt gaaatccgct
     6181 ggttaagcgc tccggcgtcg ctggcgaatc tggtattact cggttggtta ctcggcgtgc
     6241 aatatgcccg tgcgccagta attttgttag tggtcggcaa tatcctcaac attgtgctgg
     6301 atgtctggct ggtgatgggg ctgcatatga acgtgcaggg cgcggcgctg gcgacggtta
     6361 ttgcggaata tgcaacattg ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac
     6421 gcggaatttc cggcgaaatg ctgaaaactg cctggcgagg aaacttccgt cgcttgctgg
     6481 cgcttaaccg cgatatcatg ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca
     6541 ccgtacttgg tgcgcgactg gggagtgaca ttatcgctgt taacgcggtt ctgatgacgc
     6601 tactcacctt taccgcctat gcgctggatg gttttgccta cgcggttgaa gcacattccg
     6661 gtcaggcata cggtgcgcgc gacggtagcc agttgctgga tgtctggcgg gcagcgtgcc
     6721 gccagtcggg gatcgtagcg ctactgtttt cggtggttta tttgctggca ggggaacaca
     6781 tcattgcgtt actgacgtcg ttaacccaga ttcaggagct ggccgaccgc tatcttatct
     6841 ggcaggtgat tttgccgttg gttggcgtct ggtgttatct gctggacggc atgtttatag
     6901 gcgcaacgcg tgccgccgaa atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc
     6961 tgacgctcct tacgctgccg tggctgggta atcatggttt gtggctggca ttaaccgtct
     7021 ttctggcgtt gcgcgggctt tctctggcgg ctatctggcg gcgtcactgg cgcaacggta
     7081 cctggtttgc cgcaacgtga cggttaaaaa ttctgaataa attatcctaa gccaaattgc
     7141 tgactacact taatctcacg ttcagaagaa aagtgaacgt actctcattc acaacctaac
     7201 gatgaggtct tgattatgaa taaagatgaa gccggcggta actggaaaca gtttaaaggt
     7261 aaagtgaaag agcaatgggg caaactgacc gatgatgata tgacgatcat tgaaggtaaa
     7321 cgtgatcaac tggtcggtaa aatccaggaa cgttatggtt atcagaaaga tcaggcagaa
     7381 aaagaggtcg tggattggga aacccgcaat gaatatcgct ggtaattaat ccctcctgcc
     7441 cgacgtgtac aaggatgtac gccctcttaa cgcggtttct ttttcacctg cacagagtga
     7501 tcatgctggc actgttcagg atgacgacac gcttccactt ccacacatgc cgcacagagc
     7561 ccatgtgctt caatcacatt atgccgcagg gcaaacccca tttttgccgc cagcgtatgc
     7621 ataatgtctt ccacgccttc tgcacactct tctttcaccg cgccgcagcg atcgcaaata
     7681 aacatggctg aagtatgagt gggctggtcg aacagatgac agagcacata actgttggtg
     7741 gattccacct tatgcacaaa accttgctca agcagaaaat ccagcgcgcg ataaaccgtt
     7801 ggcggcttgg cttgcggttc agcttcgcgc agtaaatcaa gcagatcata agcgctgata
     7861 gcgccatcct gcagactcat caggcgcaac acttccaggc gctgtggggt caggcgcaca
     7921 ttacgctgcg cgcagatttt ttcagcctgc gctaataact cctgcgttgt ggtcttttcc
     7981 atcgggcatc tcaaagtggg ggttaagaat gtccttactt taccatgttc caggaaaaac
     8041 accgagaacc cttaatgttg ccgtaatgtt gattttttga tttgtaggta aggtgttgtg
     8101 ttgccttgtc gtaccatt
//
LOCUS       DAZFFI010000165         8039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFFI010000165 DAZFFI010000000
VERSION     DAZFFI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.167"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..318
                     /gene="ymiA"
                     /locus_tag="ACHJ12_004550"
     CDS             178..318
                     /gene="ymiA"
                     /locus_tag="ACHJ12_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4992236.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            321..488
                     /gene="yciX"
                     /locus_tag="ACHJ12_004551"
     CDS             321..488
                     /gene="yciX"
                     /locus_tag="ACHJ12_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4992237.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            602..697
                     /gene="ymiC"
                     /locus_tag="ACHJ12_004552"
     CDS             602..697
                     /gene="ymiC"
                     /locus_tag="ACHJ12_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4992238.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            861..3536
                     /gene="acnA"
                     /locus_tag="ACHJ12_004553"
     CDS             861..3536
                     /gene="acnA"
                     /locus_tag="ACHJ12_004553"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4992239.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3600..4190)
                     /gene="ribA"
                     /locus_tag="ACHJ12_004554"
     CDS             complement(3600..4190)
                     /gene="ribA"
                     /locus_tag="ACHJ12_004554"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4992240.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4360..5124
                     /gene="pgpB"
                     /locus_tag="ACHJ12_004555"
     CDS             4360..5124
                     /gene="pgpB"
                     /locus_tag="ACHJ12_004555"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4992241.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5273..5581
                     /gene="lapA"
                     /locus_tag="ACHJ12_004556"
     CDS             5273..5581
                     /gene="lapA"
                     /locus_tag="ACHJ12_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4992242.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5588..6757
                     /gene="lapB"
                     /locus_tag="ACHJ12_004557"
     CDS             5588..6757
                     /gene="lapB"
                     /locus_tag="ACHJ12_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4992243.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6950..7687
                     /gene="pyrF"
                     /locus_tag="ACHJ12_004558"
     CDS             6950..7687
                     /gene="pyrF"
                     /locus_tag="ACHJ12_004558"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4992244.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7687..8013
                     /gene="yciH"
                     /locus_tag="ACHJ12_004559"
     CDS             7687..8013
                     /gene="yciH"
                     /locus_tag="ACHJ12_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4992245.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1969 a         1885 c         2156 g         2029 t
ORIGIN      
        1 cccgcctcgc aaactccagt tttcaaatat ctatttcagg tcaaaagatt gaattattca
       61 ctggagacga ttcgtaaatt cgctggattt ttgcctaaag tttctttagg atttgtctca
      121 tcgtataatt aattacacca atcgttcaac gtcttgctga taagtgatgg cgattatatg
      181 aggttagcaa tgccttctgg aaatcaggaa ccccgccgcg atcctgaatt aaaacgtaaa
      241 gcctggctgg cggtttttct tggttctgca cttttctggg tggttgtcgc actgctgatt
      301 tggaaagtgt ggggataact atggtcggtc aggagcaact ggagtcgtca ccattatgcc
      361 agcatagtga caatgaaaca gaaacgaaac gggaatgttc cgtcgttatt ccagacgact
      421 ggcaactaac atcgcagcag caagccttta tagaactgtt tgctgaagat gatcagccga
      481 aacaataatt atcatcattc ttattaccca tttttaatga attacagggc ttttaataca
      541 ccgcagcaat aacaggttga gttatctcaa cacaaagtaa taaccgttaa gggtgtagcc
      601 tatgatcaac acaaatatga aatattggtc ctggatgggc gcgttttctc tgtcgatgct
      661 cttctgggcc gaactcctct ggatcattac tcactgatcc ttgaccccgc tgcggcgggg
      721 ttgtcatttg ctttgccaca aggtttctcc tcttttatca atttgggttg ttatcaaatc
      781 gttacgcgat gtttgtgtta tctttaatat tcaccctgaa gagaatcagg gcttcgcaac
      841 cctgtcatta aggaggagct atgtcgtcaa ccctacgaga agccagtaag gacacgttgc
      901 aggccaaaga taaaacttac cactactaca gcctgccgct tgctgctaaa tcactgggcg
      961 atatcacccg tctgcccaag tcactcaaag ttttgctcga aaacctgctg cgctggcagg
     1021 atggtaactc ggttaccgaa gaggatatcc acgcgctggc aggatggctg aaaaatgccc
     1081 atgctgaccg tgaaattgcc taccgcccgg caagggtgct gatgcaggac tttaccggcg
     1141 tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata
     1201 ctgcaaaggt taacccgctc tcaccggtcg acctggtcat tgaccactcg gtgaccgtcg
     1261 atcgttttgg tgatgatgag gcatttgaag aaaacgtacg cctggaaatg gagcgcaacc
     1321 acgaacgtta tgtgttcctg aaatggggaa agcaagcgtt cagtcggttt agcgtcgtgc
     1381 cgccaggcac aggcatttgc catcaggtta acctcgaata tctcggcaaa gcagtgtgga
     1441 gtgaattgca ggacggtgaa tggattgctt atccggatac actcgttggt actgactcgc
     1501 acaccactat gatcaacggc cttggcgtgc tggggtgggg cgtgggtggg atcgaagcag
     1561 aagccgcaat gttaggccag ccggtttcca tgcttatccc ggacgtagtg ggctttaaac
     1621 ttaccggtaa attacgtgaa ggtattaccg ccacagacct ggttctcact gttacccaaa
     1681 tgctgcgcaa acatggcgtg gtggggaaat tcgtcgaatt ttatggtgat ggtctggatt
     1741 cactaccgct ggcggatcgc gccaccattg ccaatatgtc gccagaatat ggtgccacct
     1801 gtggcttctt cccaatcgat gctgtaaccc tcgattacat gcgtttaagc gggcgcagcg
     1861 aagatcaggt tgagttggtc gaaaaatatg ccaaagcgca gggcatgtgg cgtaacccgg
     1921 gcgatgaacc gatttttacc agtacgttag aactggatat gaatgacgtt gaagcgagcc
     1981 tggcagggcc aaaacgccca caggatcgcg ttgcactgcc cgatgtacca aaagcatttg
     2041 ccgccagtaa cgaactggaa gtgaatgcca cgcataaaga tcgccagccg gtcgattatg
     2101 ttatgaacgg acatcagtat cagttacctg atggcgctgt ggtcattgct gcgataacct
     2161 cgtgcaccaa cacctctaac ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag
     2221 ccgtaactct gggcctcaag cggcaaccgt gggtcaaagc gtcgctggca ccgggttcga
     2281 aagtcgtttc tgattatctg gcaaaagcga aactgacacc gtatctcgac gaactggggt
     2341 ttaaccttgt gggatacggt tgtaccacct gtattggtaa ctctgggccg ctgcccgatc
     2401 ctatcgaaac ggcaatcaaa aaaggcgatt taaccgtcgg tgcagtgctg tccggcaacc
     2461 gtaactttga aggccgtatc catccgctgg ttaaaactaa ctggctggcc tcgccgccgc
     2521 tggtggttgc ctatgcgctg gcgggaaata tgaatatcaa cctggctgct gagcctattg
     2581 gtcatgatcg taaaggcgac ccggtttatc tgaaagatat ctggccatcg gcacaagaaa
     2641 ttgcccgtgc ggtagaacaa gtctccacag aaatgttccg caaagagtac gcagaagttt
     2701 ttgaaggcac agcagagtgg aaggaaatta acgtcacacg atccgatacc tacggttggc
     2761 aggaggactc aacctatatt cgcttatcgc ctttctttga tgaaatgcag gcaacaccag
     2821 caccagtgga agatattcac ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca
     2881 ctgaccatat ctctccggcg ggcagtatta agcccgacag cccagcgggt cgatatctac
     2941 aaggtcgggg tgttgagcga aaagacttta actcctacgg ttcgcggcgt ggtaaccatg
     3001 aagtgatgat gcgcggcacc ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg
     3061 ttgaaggggg gatgacgcgg catttacctg acagcgacgt agtctctatt tatgatgctg
     3121 cgatgcgcta taagcaggaa caaacgccgc tggcggtgat tgccgggaaa gagtatggat
     3181 caggctcaag ccgtgactgg gcggcaaaag gtcctcgtct gcttggaatt cgtgtggtga
     3241 ttgccgaatc gtttgaacga attcaccgtt cgaatttaat tggcatgggc atcctgccgc
     3301 tggaatttcc gcaaggcgta acgcgtaaaa cgttaggact aaccggggaa gagaagattg
     3361 atattggcga tctgcaaaat ctacaacccg gtgcgacggt tccggtgacg cttacgcgcg
     3421 cggatggtag ccaggaggtc gtaccctgcc gttgtcgtat cgacaccgcg acggagttga
     3481 cctactacca gaacgacggc attttgcatt atgtcattcg taatatgttg aagtaacaag
     3541 tatttgcttg ccggttattt tgcttccggc aagcaaacta attacacaat gcaagagggt
     3601 tatttgttca gcaaatggcc cattttctcg gctttggtat cgagataatg ttcgttattg
     3661 gggttacgac ctacaatcaa tggtacgcgt tcaacaatat taatccctgc ttcggtcaga
     3721 atttcgactt ttttcgggtt attggttaac aagcggactt cattgacgcc aaggagtttg
     3781 aacatatcag cgcaaagagt gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta
     3841 gcctctacgg tatcgtaacc ttgatcctgc agtgcgtaag cgcggatttt attcagcaga
     3901 ccaatgttac gaccttcctg acggtgatac agcaaaatac cacggccttc ctcggcaatt
     3961 tgcgtcaatg ccgcttcgag ctggaagcca caatcgcagc gcaagctgaa cagggcgtca
     4021 ccggtcagac attcggaatg gacgcgcgca agtaccgggg tatgcccgga aatatcgcca
     4081 tagactagcg cgacatgatc gtgtccggtt gccagttctt caaatcccac catcaggaaa
     4141 tcgccccatg gggttggcag tttggcttct gccacacgtt taagctgcat gaaattctcc
     4201 agataatgct ggttctgtat tggcttattt tgccataacg agaaggggtt cacctaatca
     4261 cgacgcgtcg atcgttcacg gaatggcaca aatctgtcaa tttttcctgg aactggcgtt
     4321 ttcagttatg attgtgggac ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt
     4381 accgcagtgg gagctgcact attgcttgtc atgccagtag ccgtatggat ttctggctgg
     4441 cgttggcaac ctggagaaca aagttggcta ctaaaagcgg ctttttgggt tactgaaact
     4501 gtcacccagc cctggggcgt cattacacat ttgattttat tcggctggtt tctctggtgt
     4561 ctgcgttttc gcattaaggc tgcctttgta ttatttgcca ttctggcggc cgcaatcctt
     4621 gtgggacaag gcgttaaatc ctggatcaaa gacaaagtcc aggaaccacg accttttgtt
     4681 atctggctgg aaaaaacaca tcatattccg gttgatgagt tctacacttt aaagcgagca
     4741 gaacgcggaa atttagtgaa agaacagttg gctgaagaga aaaatatccc acaatatttg
     4801 cgttcacact ggcagaaaga gacggggttt gcctttcctt ccggtcacac gatgtttgct
     4861 gccagttggg cactgctggc cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt
     4921 gctatcttgc tggtctgggc aacgggagtc atgggaagcc gcctgctgct cgggatgcat
     4981 tggccacgcg atctggtagt agctacgttg atttcgtggg cgctggtggc ggtggcaacg
     5041 tggcttgcgc aacgaatttg tgggccatta acaccacctg cggaagaaaa tcgcgaaata
     5101 gcgcaacgag aacaagaaag ttaacgctgg ttgattttcc gaatttagcc cttaaatcat
     5161 caacaatgcg cgtggatgcc atttcgcaga cggcgcgaaa atggtacttt aaagggctat
     5221 tgcggtaagt tgaccataat ttattcgctc taaccacata acgggaagta atgtgaaata
     5281 tttactcatt ttcttactgg tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca
     5341 gaacgatcaa caggtgacgt ttaattatct gttagcgcaa ggggagtacc gtatttccac
     5401 attgctggcg gtattgtttg ctgcggggtt tgctatcggt tggttgattt gtggcctgtt
     5461 ctggctgcga gttcgtgttt ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa
     5521 ccagctttca cccgcgactg acgtggctgt agtgccgcac tcgtcagcgg cgaaggaata
     5581 actttctatg ctggagttgt tgtttctgct tttgcctgta gccgctgcct atggctggta
     5641 tatgggccgc agaagtgcgc aacaaaacaa gcaagatgaa gccaaccgct tgtcgcgtga
     5701 ttacgtagcg ggggttaact tcctgcttag taatcaacag gataaagcgg tagacctgtt
     5761 tctcgatatg cttaaagagg atacaggcac cgttgaagcc caccttacgc tcggaaacct
     5821 gttccgttcg cgtggcgaag ttgatcgcgc tattcgcatc catcagaccc taatggaaag
     5881 cgcctcgctg acctatgaac agcgtctgtt ggcgattcaa caactggggc gtgattacat
     5941 ggccgccggg ttatacgacc gcgcggaaga catgttcaat cagctgaccg atgaaactga
     6001 cttccgcatt ggcgcgctgc aacagttgct acaaatctac caggctacca gtgagtggca
     6061 gaaagcaatt gatgttgccg aacgcctggt gaagctgggt aaagataaac agcgcgtcga
     6121 aattgcccat ttctactgtg agttagccct gcagcatatg gccagcgacg atctcgatcg
     6181 tgccatgacc ttgctaaaaa aaggggcggc ggcagataaa aacagcgccc gcgtatccat
     6241 catgatggga cgcgtgttta tggcgaaagg agaatacgcc aaagccgtcg aaagtttgca
     6301 acgcgtgata tcccaggaca gagaactggt cagcgaaacg ctggaaatgt tgcaaacctg
     6361 ctatcagcag ttgggtaaaa ctgccgaatg ggcagaattc ctgcaacgcg cggtggaaga
     6421 gaacaccggt gccgatgctg aattgatgct tgctgatatc atcgaagcgc gcgacggtag
     6481 tgaggccgca caggtctata ttacgcgcca gcttcagcgt catccgacca tgcgtgtgtt
     6541 ccataagtta atggattacc acttaaatga agcggaagaa gggcgtgcca aagagagcct
     6601 gatggtgctg cgtgacatgg ttggcgaaaa ggtacgtagt aagcctcgtt atcgctgcca
     6661 gaaatgtggt tttaccgcat acaccctcta ctggcattgt ccgtcttgtc gggcctggtc
     6721 aaccattaaa ccgattcgcg gtcttgatgg cctgtaattt ttaaaaaaat ccgactttag
     6781 ttacaacata ctagttattg atgttccatt gtgctccggc aacgacggcg cagaaaaagc
     6841 ctgccagggg aaaaatcgca actgttaatt ttttatttcc acgggtagaa tgctcgccgt
     6901 ttacctgttt cgcgccactt ccggtgccca tcatcaagaa ggtctggtca tgacgttaac
     6961 tgcttcatct tcttcccgcg ctgttacgaa ttctcctgtg gttgttgccc ttgattatca
     7021 taatcgtgat gacgcgctgt cctttgtcga caagatcgac ccacgcgatt gtcgtctgaa
     7081 ggtcggcaaa gagatgttta cattgtttgg gccacagttt gtgcgcgaac ttcaacagcg
     7141 tggttttgat atctttcttg acctgaaatt ccacgatatt cctaacactg ctgcgcacgc
     7201 tgtcgctgct gcagctgact taggcgtgtg gatggtgaat gttcatgcct ctggtggggc
     7261 gcgtatgatg accgcagcgc gtgaggcact ggttccgttt ggcaaagatg caccgctttt
     7321 gattgctgtg acagtgttga ccagcatgga agccagcgac ctggtcgatc ttggcatgac
     7381 actgtcacct gcagattatg cagaacgtct ggcggcactg acgcaaaaat gtggccttga
     7441 tggtgtggtg tgttctgctc aggaagctgt gcgctttaaa caggtattcg gtcaggagtt
     7501 caaactggtt acgccgggca ttcgtccgca ggggagtgaa gctggtgacc agcgccgcat
     7561 tatgacgcca gaacaggcgt tgtcggctgg tgttgattat atggtgattg gtcgcccggt
     7621 aacgcaatcg gtagatccag cgcagacgct gaaagcgatc aacgcctctt tacagcggag
     7681 tgcatgatga gtgattccaa cagccgtctg gtctactcaa cggaaaccgg acgtattgat
     7741 gagcccaaag cggcccctgt acgccctaaa ggcgacggtg tggtgcgtat tcagcgtcag
     7801 accagtggac gtaaaggtaa gggcgtttgc ctgattaccg gtgtcgatct cgatgatgcc
     7861 gaactgacaa aacttgcagc ggaactgaag aaaaaatgcg gctgcggcgg agcagttaaa
     7921 gatggagtta ttgaaatcca gggcgataag cgtgatttat taaaatcact acttgaagcg
     7981 aaagggatga aggtaaaact cgcaggtggt taacataaaa agccacggat atatccgtg
//
LOCUS       DAZFFI010000166         8005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFFI010000166 DAZFFI010000000
VERSION     DAZFFI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.168"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(314..799)
                     /gene="sixA"
                     /locus_tag="ACHJ12_004560"
     CDS             complement(314..799)
                     /gene="sixA"
                     /locus_tag="ACHJ12_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4992246.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1002..3146)
                     /gene="fadJ"
                     /locus_tag="ACHJ12_004561"
     CDS             complement(1002..3146)
                     /gene="fadJ"
                     /locus_tag="ACHJ12_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4992247.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3146..4456)
                     /gene="fadI"
                     /locus_tag="ACHJ12_004562"
     CDS             complement(3146..4456)
                     /gene="fadI"
                     /locus_tag="ACHJ12_004562"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4992248.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTVFDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4636..4920)
                     /locus_tag="ACHJ12_004563"
     CDS             complement(4636..4920)
                     /locus_tag="ACHJ12_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4992249.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5292..6632
                     /gene="fadL"
                     /locus_tag="ACHJ12_004564"
     CDS             5292..6632
                     /gene="fadL"
                     /locus_tag="ACHJ12_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4992250.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6998..>8005
                     /gene="yfdF"
                     /locus_tag="ACHJ12_004565"
     CDS             6998..>8005
                     /gene="yfdF"
                     /locus_tag="ACHJ12_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO4992251.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQ"
BASE COUNT         2158 a         2045 c         1821 g         1981 t
ORIGIN      
        1 acttttgaca atataatata taaacactat agccagaatt taatcaagtt tcaattacat
       61 cctgcctgat tattcaactc tattatctgt attaataaat gacaatgagt gtcaaacaga
      121 cattattcat ttcaacatgt aactttgggc aaaatcaaga tacgcgcaga agaataattt
      181 ccgaatgaat gtagaaagtg ttttatttat agataaggag agtcttcgat agtagtgagg
      241 ttgacagata tccgctgcct gatgagcaat tatgcccaat gaatctacct cattgggcat
      301 aatttggaac acatcagata gcttttgcca tcttcagatt acacgggctc atctgccagt
      361 tgaatgtacc gtttccgctt tcatcaagcg ttacgctggc aatggccgag gtggtaaaca
      421 taggcggcgt ttcgcccgga cataactcgg caaccagata cccgactaac ggtaagtgag
      481 agatcaccag taccgaagca acaccttcat tggtcaacgc ctgcaaatag gcactgacca
      541 gaccaacatc gccgcagggc gttaactccg gcagaacttc tgcactggaa ggcaggttca
      601 aacaatcccc tacttcttcc agtgtttgct cggctcgcag gaacgggctc accagaacgc
      661 gttcgatttc cactttttga cctttcagcc agttcgccat caggcgagat tcgtcacaac
      721 cattagtggt cagaggacga acggaatcac tggcggcatc gagggctgcg tcgccgtgac
      781 gcatgataaa aacttgcata ttgcaccgct tttgttaacc agattcgccc gttttctttc
      841 atctgaaacc agagaaagaa aacggtggcc ggcattgtgc cttatccatt caccgaatga
      901 aacgctgttt tttacctcaa tggcgtaagt atagtcaata tgcgtttaca ttttgaccaa
      961 cactccgcca ttcagcgcgg attcatatag ctttgacctt cttattgcag gtcagttgca
     1021 gttgttttcc aaaaactttc cccacgcgcg cccatctcga ccaaacgctc gcaaggggta
     1081 aaacgggaac catactgcgt ggcaagtcgt tgcattattg caaccacttc gcccgcgccg
     1141 agagaatcga tatagcggaa cggtccaccg agaaatggcg gaaaaccaat gccaaatacc
     1201 gcaccaatat ccccgtcacg cacgctacgg ataacctgct catcaacaca acgtactgct
     1261 tcattcagca tcaacatcac acaccgttca gcaacctgcg gtgcggagag tcgcccctgc
     1321 ccttgtgcgc caatgagcgg gtaaatggcg ggatcgacct gttttttgct tttacgccct
     1381 ttctgaccat aaagatagaa accccgacca ttttttctgc ctttgcgatc gtcgttcaaa
     1441 attgaagaaa caacatttgc aggcgcgcta aaacgttctc cataagcggc ttccagtaca
     1501 ggaataattt tagtcccggt gtcgattcct acctcatcca aaagttggat tggacctacc
     1561 ggaaaaccaa atttcactag cgcagcatca atgtgctcta cccgttcacc ttcggtcagc
     1621 atgcggatag cttcattaat gtaaggcgct aagatgcgat tgacgtaaaa cccggctttg
     1681 tcacgcacga caatgggcgt tttgccctgt tttttcgcca gttttaccgt agtggcgatg
     1741 gtttgcgctg atgtacccgc atgaggaata atctccacca gcggcatttt ttccaccgga
     1801 ctgaagaaat gcaggccgat aacttgctca ggtcgcgtgg cgtgagcggc gatatcacca
     1861 atcggcaaag atgacgtatt cgaagcaaag atggtatgag cggcgcaatt ttgctccact
     1921 tccgccacca tctgttgttt taattcgaga ttttcaaaca ccgcttcaat aatcagatcg
     1981 cgatgggcaa agccgcgata gtccgtcgtt ccggagatta atgccagctg tttgtcacgt
     2041 tcgctggctt tgagatgacg acggcgaact ttgccctcca gctgatccca actgtacttc
     2101 agcgcatgat ttatgccctg cgggttgata tctttaattc tgaccggaag ccccgcttta
     2161 caagcagtga cataagcaat accgccgccc atcaagccac cacctaaaat tcccacgctg
     2221 tttaatggcg caggcggtgc atcactaccg ggatctttct tcacttccgt actggcaaaa
     2281 aagatactac gcagcgcctg cgattgtggc gtcatcgcca gttcgccaaa cgcccgagct
     2341 tcggcgtcat aaccgctgct ggtgccctgc gctaatcccg tttcaacaac ctccaggatg
     2401 cgttctgtcg ccggataatt gccttgagtt ttgtgttctg ttttcttacc gaccattttg
     2461 aacagcagcg cacgacctaa cggccccgcc agaatacgct cgcgtacagg tagagggcgg
     2521 gaagatgggc gatccttctt cgccagctca acagcgactt ccagcaaaat ggagtgcgga
     2581 acaacgtcat ccaccagccc cagctttaat gcctgtttcg cccgaagttg ttttccggtg
     2641 aggatcatct ctaatgctgt gctgacgcct atcagacgcg gtaaacgctg ggtgccgcct
     2701 gaaccgggta acaatccaag ttgtacttca ggcaaaccga gcaccgtttt aggatcgtca
     2761 gtacaaacgc gaccgtggca cgccagcgcc aactccagcc caccacccag gcaagcgcca
     2821 tgaatagccg cgataaccgg aatgggcaaa gcatgaatct ccgccatcaa ctgttggccc
     2881 tgccgcgcca gagcttccgc ttcttgcgcc gttttgcagt tgccgatcat gttgatgtct
     2941 gcgccagcaa tgaagttgtc cggtttagcg gagacaaaca ccacgcctcg caactctttg
     3001 ttttcacgga gttgcttaat aatggcgcgc acctgcgagg caaactccgc cttcagggta
     3061 ttcattttct cacccggtac gtcgatggtg ataacggcaa tgttgtccag acgaacatta
     3121 agggtaaacg ctgatgtcat ttccattatt ccgcctccag aaccattgcc gcgccaagcc
     3181 caccggcagc acaggcggta actaaaccaa atccaccgcc gcgacggcga agttcatgca
     3241 atgtctgggt aatcatccgt gcgccggtcg ccgcgaaggg atgcccgtaa gcaatcgaac
     3301 cgccaagcac gttaaatttg ctatcgtcca cttcgccagt ggcatgtgca cgccccagta
     3361 catcacgagc aaaacgttcg ctgcccagca actgaatatt cgccagcgtc tgagctgcaa
     3421 aggcttcgtg catatcgaac actgtcagat cgctcatcgt caaaccggca cgttccagcg
     3481 ccagcggcgt tgaccaggcc ggaccgagca acatgtcctg ccagacatca atcgcagtaa
     3541 atgcgtagct gcgcagatac cccagcggta ccagccctaa ttctttcgcc cgggattcag
     3601 tcatcaggat caccgccgct gcgccatcgg tcagcggcgt actgttcgcc gccgttaccg
     3661 ttccgtgttt gcgatcaaac gccgggcgca gctttgcgta atcggcaagc gaggaattac
     3721 cgcgaatatt gttgtcttcg acaagcggtt gtttataagg agggataaag gcagtcatca
     3781 cctcttcttt gagtttaccg tctgaccacg cctgagcggc acgctgatgc gaacggtgcg
     3841 ctaatgcatc ttgctgttct cgggtgatgc cgtaggtttt cgccatttgt tcggcggtgt
     3901 cgcccatccg caagccagta gaatattctg ctaccgcagg cggtacgggc attaagtcgc
     3961 gcaaacgcag gcgagagaag agtttcaggc gctggctcat ggttcgagct ttgttgacat
     4021 caaccagcac gcgcgccagt tttttactga cgccaattgg caatacggaa gaggaatctg
     4081 ccccaccggc aatccccgct cggatagttc ccgccatcag gctttctgcg acgtttgcaa
     4141 ccgcctggaa actggtagcg caagcgcggc tgacgctgta agcatcggta tgcacattca
     4201 ttccggtacc gagaacaatt tcacgcgcaa tgttgggggc ttcaggcatt tgtacgacct
     4261 gaccaaagac cagctgttca atcacttcag cggggatctc gctgcgtgcc agcagttcgc
     4321 ctaccaccat cttccctaaa tcaaccgcgg gaatgccatg aaaagccgtc ccctggcggg
     4381 caaaaggcgt acgtaaaccg ctaacaatgg cgatacgatc gccctggcgg gtaaccagcg
     4441 gtaaaacctg acccataaca ctcccctgta aaaaaataaa taaagtggtc tgacctgatc
     4501 atagtcttaa ccattttttt acatttagcc aagcggagaa aaaagaaagt gggagctatg
     4561 acacagtgaa gacaggagaa agaaaagaaa acgcccctgc cagcaactga caggggcgaa
     4621 cgtacccgag aggaattaac gcagacccag ctggaagatc agcatttcag cttcgcaggc
     4681 aaaagtgaaa tcgatatcca ggcgcacacc gtcagactct tcagtgaaag tcggggagat
     4741 tttgcacggt tcggattcca cgctacgggc tttttcagtc agcgccgcca gcgtttgttc
     4801 tgcttctgcg cggtttgcaa acacgcggct gtaagacgcg gtgcagtcgg agttgtccat
     4861 aatggtgcca acatccatac agcagcaaac cggggtttca tcagcactac atttactcat
     4921 cgttgatttc ctctgtatgt gcacccaagg tgccagataa acgttgtgga tattttacgc
     4981 ttccggaaag tgctgctcca gttgttaatt ctgcaaaatc ggataagtga ccgaaatcac
     5041 acttaaaaat gatctaaaac aaaattcacc cgaatccgcg agtgcgcaac ctccaaattt
     5101 tgccagctgg atcgcgtttc ttagatcata tttgaaaaaa gatggaaaca tacttgcaac
     5161 attccagctg gtccgaccta tactctcgcc actggtctga tttctaagat gtacctcaga
     5221 ccctacactt cgcgctcctg ttacagcacg taacatagtt tgtataaaaa taaatcattg
     5281 aggttatggt catgagccag aaaaccctgt ttacaaagtc tgctctcgca gtcgcagtgg
     5341 cacttatctc cacccaggcc tggtcggcag gctttcagtt aaacgaattt tcttcctctg
     5401 gcctgggccg ggcttattca ggggaaggcg caattgccga tgatgcaggt aacgttagcc
     5461 gtaaccccgc attgattacc atgtttgacc gcccgacttt ttctgcgggt gcggtttata
     5521 ttgacccgga tgtgaatatc agcggaacgt ctccgtctgg tcgtagcctg aaagccgata
     5581 acatcgcgcc tacggcatgg gttccgaaca tgcactttgt tgcaccgatt aacgaccaat
     5641 ttggttgggg cgcttctatt acctctaact atggtctggc aacagagttt aatgatactt
     5701 atgcaggcgg ctctgtcggg ggtacaactg accttgaaac catgaacctg aacttaagcg
     5761 gtgcgtatcg cttaaataat gcatggagct ttggtcttgg tttcaacgcc gtctacgctc
     5821 gcgcgaaaat tgaacgtttc gcaggcgatc tggggcagtt ggttgctggt cagattatgc
     5881 aatctcctgc cgggcaaact cctcaagggc aagcattggc agctaccgcc aacggtatcg
     5941 acagtaatac caaaatcgct catctgaacg gtaaccagtg gggctttggc tggaacgccg
     6001 gaatcctgta tgaactggat aaaaataacc gctatgcact gacctaccgt tctgaagtga
     6061 aaattgactt caaaggtaac tacagcagcg atcttaatcg tgcgtttaat aactacggtt
     6121 tgccaattcc taccgcgaca ggtggcgcaa cgcaatcggg ttatctgacg ctgaacctgc
     6181 ctgaaatgtg ggaagtgtca ggttataacc gtgttgatcc acagtgggcg attcactata
     6241 gcctggctta caccagctgg agtcagttcc agcagctgaa agcgacctca accagtggcg
     6301 acacgctgtt ccagaaacat gaaggcttta aagatgctta ccgcatcgcg ttgggtacca
     6361 cttattacta cgatgataac tggaccttcc gtaccggtat cgcctttgat gacagcccag
     6421 ttccggctca gaatcgttct atctccattc cggaccagga ccgtttctgg ctgagtgcag
     6481 gtacgactta cgcatttaat aaagatgctt cagtcgacgt tggtgtttct tatatgcacg
     6541 gtcagagcgt gaaaattaac gaaggcccat accagttcga gtctgaaggt aaagcctggc
     6601 tgttcggtac taactttaac tacgcgttct gataacacgt tcgcctggat aaagtcacct
     6661 gcatagcagg tgactttaac tcccccactt caccgaaagt agtgtcctca ttgcttacct
     6721 cctgagtttt gcaaacagcc tgatggcagc ttgccctttc aaatcaataa gcggtatcca
     6781 cagacaaatc tgaagggaaa ggcatatttt caggcgttct gctcgtcctt ctcaaagagt
     6841 ttacttttct gaatttccag gatactcccc ccccctggct attgtgcgct catacactca
     6901 aattaaagat aggttctaaa taaatgagcg ttttttgata gtctatttca ttaggtaata
     6961 tatatttgta acaaatcaat caaaatggaa taaaatcatg ctaccatcta tttcaatcaa
     7021 caataccagc gcaacttacc cagaatccat taatgaaaat aacaatgatg aagttaatgg
     7081 attagtacaa gagctcaaaa acctttttaa tggtaaggaa ggaataagca cctgtattaa
     7141 acatctactt gagcttataa aaaacgccat acgagtaaac gacgatcctt atagatttaa
     7201 tattaataat tcctcagtta cttatattga tattggctcc aatgatacag accatattac
     7261 tattggtatc gacaaccaag aaccaataga attacctgcg aactataaag acaaagaact
     7321 cgtccgtact atcattaatg acaacatagt tgagaagact catgatatca ataacaagga
     7381 aatgatcttc agcgcattaa aagaaatata tgatggagat cctggtttta tcttcgataa
     7441 gatatcacac aaactcagac atacggtaac ggaatttgat gagagcggga atagcgaacc
     7501 aacggactta tttacctggt acggtaaaga taaaaaaggc gactctctcg ctattgtaat
     7561 taaaaataaa aacggaaatg attacttatc tctcggttac tacgatcagg acgactacca
     7621 cattcaaaga ggaattcgta ttaatggtga tagtctcacc caatattgta gtgaaaacgc
     7681 caggagtgct tcagcgtggt ttgaaagcag caaagctatc atggcagaat catttgcaac
     7741 aggttccgat catcaggttg taaacgagct caacggggaa agactgagag aacctaacga
     7801 agtttttaaa cgtttaggtc gagcaataag atataatttt caagtggacg atgcaaaatt
     7861 tagacgcgat aatgtaaaag aaataatttc tactttattc gctaacaaag ttgacgttga
     7921 ccatcctgaa aataaataca aagactttaa ggatctcgaa gataaagttg aaaaaaggct
     7981 tcaaaatcgc cagacggaat atcaa
//
LOCUS       DAZFFI010000167         7995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFFI010000167 DAZFFI010000000
VERSION     DAZFFI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.169"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..213
                     /gene="kdpF"
                     /locus_tag="ACHJ12_004566"
     CDS             124..213
                     /gene="kdpF"
                     /locus_tag="ACHJ12_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4992252.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            213..1886
                     /gene="kdpA"
                     /locus_tag="ACHJ12_004567"
     CDS             213..1886
                     /gene="kdpA"
                     /locus_tag="ACHJ12_004567"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4992253.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1909..3957
                     /gene="kdpB"
                     /locus_tag="ACHJ12_004568"
     CDS             1909..3957
                     /gene="kdpB"
                     /locus_tag="ACHJ12_004568"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4992254.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3966..4538
                     /gene="kdpC"
                     /locus_tag="ACHJ12_004569"
     CDS             3966..4538
                     /gene="kdpC"
                     /locus_tag="ACHJ12_004569"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4992255.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4531..7215
                     /gene="kdpD"
                     /locus_tag="ACHJ12_004570"
     CDS             4531..7215
                     /gene="kdpD"
                     /locus_tag="ACHJ12_004570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4992256.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7212..7889
                     /gene="kdpE"
                     /locus_tag="ACHJ12_004571"
     CDS             7212..7889
                     /gene="kdpE"
                     /locus_tag="ACHJ12_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4992257.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1640 a         2077 c         2369 g         1909 t
ORIGIN      
        1 agttaaatca tggcttttgc catttttata ctttttttac accccgcccg cagatttttg
       61 cgaaatcttt gcagccagaa ttctaccctt ccggtatcac ttttaggcca ctggaggtgc
      121 actgtgagtg caggcgtgat aaccggcgta ttgctggtgt ttttattact gggttatctg
      181 gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgatcg
      241 ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc
      301 tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac
      361 ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga
      421 acatgctggg gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta
      481 atccacagca gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg
      541 tcacgaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat ttcagccaga
      601 tggctggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg gtgatttttg
      661 ccctcatccg tgcgtttacc cgccagagca tgagcacact cgggaatgcc tgggtcgatc
      721 tgctacgcat cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca ctgtttttta
      781 ttcaacaagg tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc gttgaaggag
      841 cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag atgctcggta
      901 ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac
      961 tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacggcgctg tgctttgcct
     1021 ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga
     1081 tttttgtcat ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat cctcatctgc
     1141 tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc
     1201 tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg gtgattgcga
     1261 tgcatgattc gtttaccgct ctcggtggca tggtgccgat gtggctgatg caaattggtg
     1321 aagtggtgtt cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg
     1381 cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg
     1441 acgtacgcga gatgaaactg actgcactgg cgattctggt taccccgacg ctggtgctga
     1501 tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc aaccctggcc
     1561 cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac aacggcagcg
     1621 cctttgccgg attaagcgcc aactccccgt tctggaactg tttactggcg ttctgcatgt
     1681 ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta
     1741 aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc
     1801 tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg
     1861 gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa
     1921 caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa
     1981 ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg
     2041 ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt
     2101 agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg
     2161 ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc
     2221 tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat
     2281 caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt
     2341 gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg
     2401 gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct
     2461 gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc
     2521 gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg
     2581 ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg
     2641 tggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca
     2701 accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc
     2761 gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg
     2821 ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatccct
     2881 gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat
     2941 gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag
     3001 cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc
     3061 gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat
     3121 gttgaggcta acggcggtca cttccctacc gatgttgatc aaaaagtcga tcaggttgcg
     3181 cgtcaggggg ccacgccgct ggtggtggtg gaaggttccc gtgtgctggg cgttattgcg
     3241 ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc
     3301 attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat tgctgcggaa
     3361 gcgggcgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc attgattcgt
     3421 cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa cgatgctcca
     3481 gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gtactcaggc ggcgaaagaa
     3541 gcgggcaata tggtcgatct cgactctaac ccgaccaagt tgatcgaggt ggtacatatt
     3601 ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg
     3661 gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca gttaaatgcg
     3721 ctgaacatca tgcgccttca ttcgcccgac tccgccattc tcagtgcggt cattttcaac
     3781 gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt
     3841 accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg
     3901 ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt
     3961 ttaccatgcg tggcttacgt ccggcattat cgacatttat ctttctgtta ttgattactg
     4021 gcggcgttta cccgctgctg accaccgtgc tggggcaatg gtggtttccc tggcaggcca
     4081 acggttcgtt gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc gggcagaatt
     4141 ttaccggcaa cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg ccctataatc
     4201 cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat aaacaaatag
     4261 ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagtacgaac gttccggttg
     4321 aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa gcggcggtct
     4381 ggcaaatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc acgcaactga
     4441 tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg
     4501 ttgaactcaa tctggcgctg gataaacttg atgaataacg aacccttacg tcccgacccc
     4561 gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt
     4621 gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg
     4681 caagggctgg atattgtggt tggcgtggta gaaacccatg ggcggaaaga taccgccgcc
     4741 atgcttgaag ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg gcggcatatc
     4801 agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa
     4861 ctggcgcaca gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa
     4921 ctgctggaag ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg
     4981 aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt
     5041 ttcgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct gcgccagcgg
     5101 ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc
     5161 cgcaaaggca atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc cgatcgcgta
     5221 gatgagcaaa tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg gcacacgcgc
     5281 gacgcgatcc ttttatgcat cgggcataac accggcagtg agaaactggt ccgcgcggcg
     5341 gcgcggttgg cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc
     5401 ctgcatcgct taccggaaaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag
     5461 gagctgggcg cggagacggc tacactttct gatccagcgg aagaaaaagc agtagtgcgt
     5521 tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc gcgccgctgg
     5581 tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg
     5641 ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct
     5701 tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc
     5761 gttatcactt taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg
     5821 ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt
     5881 gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc
     5941 gctgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg
     6001 atcgggaacc ttactgcggg tgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa
     6061 cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac
     6121 atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg
     6181 ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat gaccccgtgg
     6241 gacgatgcca tcgcacagtg gagttttgat aaaggcctgc ctgcgggtgc gggcaccgac
     6301 acgttacccg gtgtaccgta ccagattttg ccgttaaaaa gcggtgagaa aacctacggg
     6361 ctggtggtgg tggaaccagg gaatctgcgt cagttgatga tcccggaaca gcagcgcctg
     6421 ctggagacgt ttacgctgtt agtcgccaat gcgtttgagc ggcttacgct gaccgccagt
     6481 gaagaacagg cgcggatggc aagcgaacgg gaacagatcc gcaacgctct gctggcggcg
     6541 ctttcgcatg atttacgcac gccgctcacg gtgctgttcg gtcaggcaga aatcttaacg
     6601 ctcgatctgg caagcgaagg atcgcctcac gcccgccagg ccagcgagat tcgtcagcac
     6661 gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc
     6721 ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tggtcggcag cgcgctgcaa
     6781 atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta
     6841 atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg
     6901 aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta
     6961 caactggatg tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt
     7021 gataagtttg ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct tggactggca
     7081 atttgtcggg cgatagtgga tgtacacggg ggcactatta ccgcgttcaa ccgaccggaa
     7141 ggtggtgcct gttttcgtgt tacacttccc cagcaaactg cccctgaact tgaagaattt
     7201 catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc
     7261 tgcgcacggc gctggagggc gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg
     7321 gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg
     7381 atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg
     7441 tgctttccgc acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg
     7501 attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac
     7561 gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg
     7621 atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcacg ccgattgagt
     7681 tccgcctgct ggcggtgctg ctgaataatg ccgggaaagt gctcacccag cgccagttac
     7741 tcaatcaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg
     7801 gacatctgcg acaaaaactg gaacaggatc ccgctcgccc ccgccatttc attactgaaa
     7861 ccggtattgg gtatcggttt atgccatgaa tgtttattca ataaatgcgc ttaaataatt
     7921 attaatttat tattaaacct gccaaaaata ttattatttg gcaggtttaa tttcttaacg
     7981 caaatataaa acaca
//
LOCUS       DAZFFI010000168         7982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFFI010000168 DAZFFI010000000
VERSION     DAZFFI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.170"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(207..470)
                     /gene="cedA"
                     /locus_tag="ACHJ12_004572"
     CDS             complement(207..470)
                     /gene="cedA"
                     /locus_tag="ACHJ12_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4992258.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRMEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            653..2914
                     /gene="katE"
                     /locus_tag="ACHJ12_004573"
     CDS             653..2914
                     /gene="katE"
                     /locus_tag="ACHJ12_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4992259.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3173..3931)
                     /gene="chbG"
                     /locus_tag="ACHJ12_004574"
     CDS             complement(3173..3931)
                     /gene="chbG"
                     /locus_tag="ACHJ12_004574"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4992260.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVMDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3944..5296)
                     /gene="celF"
                     /locus_tag="ACHJ12_004575"
                     /gene_synonym="chbF"
     CDS             complement(3944..5296)
                     /gene="celF"
                     /locus_tag="ACHJ12_004575"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4992261.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5401..6243)
                     /gene="chbR"
                     /locus_tag="ACHJ12_004576"
     CDS             complement(5401..6243)
                     /gene="chbR"
                     /locus_tag="ACHJ12_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4992262.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6251..6601)
                     /gene="chbA"
                     /locus_tag="ACHJ12_004577"
     CDS             complement(6251..6601)
                     /gene="chbA"
                     /locus_tag="ACHJ12_004577"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4992263.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6652..>7982)
                     /gene="chbC"
                     /locus_tag="ACHJ12_004578"
     CDS             complement(6652..>7982)
                     /gene="chbC"
                     /locus_tag="ACHJ12_004578"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4992264.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDSISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2144 a         2082 c         1786 g         1970 t
ORIGIN      
        1 ggatgaacta tcaaaaatag ctcatcatga ttagcaaaac ttaaccattt taaaataaat
       61 aaacaattaa agaaaaaaga tcacttattt atagcaatag atcgtcaaag gcagcttttt
      121 gttactggtg gtttgaatga atgtagcaat gaaatacaga atttcaggtc atgtaactcc
      181 cggcaaaacc gggaggtacg taatctttac tcagtcactt ccccttcctg gcggatctga
      241 tttgcccaac gctgggcgga ttcaggcaca gtaaacgccg gtgagcgcag aaatgactct
      301 cccatcagta cgaacgcaac atatttgcca cgcagcatcc agacatcacg aaacgaatcc
      361 atctttatcg catgttctgg cggcgcgggt tccatgcgtg ggacatagct aataatctgg
      421 cggttttgct ggcggagcgg tttcttcatt actggcttca ctaaacgcat attaaaaatc
      481 agaaaaactg tagtttagcc gatttagccc ctgtacgtcc cactttgcgt gtatttcata
      541 acaccgtttc cagaatagtc tccgaagcgg gatctggctg atggtctata gttagagggt
      601 tttttgacca aaacagcggc cctttcagta ataaattaag gagacgagtt caatgtcgca
      661 acataacgaa aagaacccac atcagcacca gtcaccacta cacgattcca gcgaagcgaa
      721 accggggatg gactcactgg cacctgagga cggatctcat cgtccagcgg ctgaaccaac
      781 accgccaggt gcacaaccta ccgcccctgg gagcctgaaa gcccctgata cacgtaacga
      841 aaaacttaat tctctggaag acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa
      901 tcagggcgtg cgcatcgccg atgatcaaaa ctcactgcgt gccggtaacc gtggtccaac
      961 gctgctggaa gattttattc tgcgcgagaa aatcacccac tttgaccatg agcgcatccc
     1021 ggaacgtatt gttcatgcac gcggatcagc agctcacggt tatttccagc catataaaag
     1081 cttaagcgat atcaccaaag cggatttcct ctcagatccg aacaaaatca ccccagtatt
     1141 tgttaggttc tctaccgttc agggtggcgc tggctctgcc gataccgtgc gtgatatccg
     1201 tggttttgcc accaagttct atactgaaga gggtattttt gacctcgttg gcaataacac
     1261 gccaatcttc tttatccagg atgcgcataa attccccgat tttgttcatg cggtaaaacc
     1321 agaaccgcac tgggcaattc cacaagggca aagtgcccac gatactttct gggattatgt
     1381 ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg atgtcggatc gcggcatccc
     1441 ccgcagttac cgcaccatgg aaggcttcgg tattcacacc ttccgcctga ttaatgccga
     1501 agggaaggca acgtttgtac gtttccactg gaaaccactg gcaggtaaag cctcactcgt
     1561 ttgggatgaa gcacaaaaac tcaccggacg tgacccggac ttccaccgcc gcgagttgtg
     1621 ggaagccatt gaagcaggcg attttccgga atacgaactg ggcttccagt tgattcctga
     1681 agaagatgaa ttcaagttcg acttcgatct tctcgatcca accaaactga tcccggaaga
     1741 actggtgccc gttcagcgtg tcggcaaaat ggtgctcaat cgcaatccgg ataacttctt
     1801 tgccgaaaac gaacaagcag cattccatcc aggtcatatt gttcctggtc tggacttcac
     1861 caacgatccg ctgttgcagg gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct
     1921 tggtgggccg aatttccatg agattccgat taaccgtccg acctgccctt accacaactt
     1981 ccagcgtgat ggcatgcatc gtatggggat cgacactaac ccggcgaatt acgaaccgaa
     2041 ctcgatcaac gataactggc cgcgcgaaac accgccggga ccgaaacgcg gcggttttga
     2101 atcatatcag gaacgcgtgg aaggaaataa agttcgtgag cgcagtccat cgtttggcga
     2161 atattattcc catccgcgtc tgttctggct aagtcagacg ccattcgagc agcgccatat
     2221 tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt ccgtatattc gtgagcgcgt
     2281 tgttgaccag ctggcgcata ttgatctcac tctggcccag gcggtggcga aaaatctcgg
     2341 tatcgaactg actgacgacc agctgaatat caccccacct ccggacgtca acggtctgaa
     2401 aaaggatcca tccttaagtt tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt
     2461 agcgatttta cttaatgatg aagtgagatc ggcagacctt ctggccattc tcaaggccct
     2521 gaaggccaaa ggcgttcatg ccaaactgct ctactcccga atgggtgaag tgactgcgga
     2581 tgacggtacg gtgttgccta tagccgctac ctttgccggt gcaccttcgc tgacggtcga
     2641 tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct gacaacggcg atgccaacta
     2701 ctacctgatg gaagcctaca aacaccttaa accgattgcg ctggcgggag acgcgcgcaa
     2761 gtttaaagca acaatcaagg tcgctgacca gggtgaagaa gggattgtgg aagctgacag
     2821 cgctgacggt agttttatgg atgaactgct aacgctgatg gcagcacacc gcgtgtggtc
     2881 acgcattcct aagattgaca aaattcctgc ctgatgggag gcggcgcaat tgcgccgcct
     2941 caaatgattt acatagtgcg ctttgtttat gccggatgcg gcgtgaacgc cttatccggc
     3001 ctacaaagtc gtataaattc aatgtattgc agtttattgg tagatactga taagcgaagc
     3061 gcatcaggca gttttgcgtt tgtcagcagt ctcaagcggc gcagttacgc cgcctttgta
     3121 ggaattaatc gccggatgca aggttcacgc cgcatctggc aaacatcctc acttacacat
     3181 cccgataact ccccaaccga taaccacgct gagcgatagc acctttcaac gacgctgatg
     3241 tcaacacatc cagctccgtt aagcgtggga aacagtaagc actctgacgg atagtattat
     3301 cgataaacgc cggatgacac atcacctcca gcgaacgatc accccgatgg cttgcgtcat
     3361 ccatcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg ctactgaatc
     3421 cctgagaact gcgcagatta gccggtaaat caccagcgtt agatagcggc tgacggtcga
     3481 tgcgcaacgc aatcccctct tcagccgcaa acctggcaac aatcgggaaa atctgcggga
     3541 acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat agctcaatga
     3601 aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa gcatcttctt
     3661 ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc ggcatagctg
     3721 tcagtggctt gcccatagta aggacaaagt gcatccctat ggccagactc ggttcatcac
     3781 gactcaactg caccgcatgg tcaatagcct gcccattcac cagcgccgtc gttgatgtga
     3841 caatcccatt gcgacaggcc tcgataatgc cgtagttctg acctttgctt aagccaaaat
     3901 catcggcatt aacaatcagt aagcgttcca taatcagcct cggttaatgt gcttttttaa
     3961 gctctgcgat gcagtcggca aaatttggca gccatttctc gtgcgccaga atcatctcgc
     4021 gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg actaaggttt agcgccagta
     4081 acacatcgtt aaattctccg ctaagtgcgg cgttgctggc agcaatctcg aagcctttaa
     4141 tggtgtgaat cagccccatc actttatcat cgaaatgcgt aatgcgcgga tgtggcgtcg
     4201 cgccatcgcg ccccagctta caggtcattt ctaccgccca gtctgccgga atattatcaa
     4261 tatgcccatg atgcgggata ttaacgtaat gttcagcttg cttgtcgttg tagatagcgt
     4321 tgatcacttc gcacgctgca tcagagtaat aagccccacc gcgctgttcc agttctttcg
     4381 gcttaacttt caactccgga tttttataca gctcaaaaag ttgtttctcg actttctgta
     4441 ctacctgtgc tcgtgcgccg cctttgtagt attcgcccat ttcaatagcc agcatctctt
     4501 tctgcttgaa gtaatacagc agataagaac atggcagcag atttaacgaa cgaattaagc
     4561 cctcactaaa tggcagatcg aaaatatttt ttacagagga cgcttttaac tgccctgacg
     4621 ccacaccatc aagcaattcg gcaaagcgcg acttgccatt taccagcaca tccttaatga
     4681 acaccatatg gttgaggccg aacagatcga tagataaatc atcgctgtct ttcagcatca
     4741 gaacatcgcg aataaacatc ttcatgccga tcggaatatt acacacgccg ataaagcgtt
     4801 taaatccggt atgacgataa acggcttcag tgaccattcc cgccgggtta gtgaagttaa
     4861 tcacccatgc attcggacaa agttcttcga catcttttac gatgtcaaaa atcaccggaa
     4921 tggtacgcag acctttaaac agaccgcccg cgccgttggt ttcctgacca agataaccat
     4981 gacttaatgg aatacgttca tccagttcgc gcgccggtaa ttggcctacg cgcagttggg
     5041 tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc cagcgtttta taaagcttca
     5101 tcgggacgcc agcgttatca atcatccgtt ggcagagttc aaaaatgata tccagtttct
     5161 ctttaccatc ttcgacatcc accagccata attcgctgac cggcaattcg tgataacgct
     5221 taataaatcc ttccagtaac tccggggtat agctgctccc gccaccaata gtgacgactt
     5281 ttaatttctg gctcataatt tctcccttca gtacagaata ctgatatctg gcatatctgc
     5341 ccccccggac ataaataatc cagcaacagg acagatatgt gaattgtcag gtataacgac
     5401 ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg acgttaattt
     5461 cttaaacgtt ttaataaaca aactcgggct actataaccg gcctcaaacg caatatccgt
     5521 cacggaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga tctcattaat
     5581 aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat attcctgtga
     5641 tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct ctttatcatg
     5701 catcttttct accgtacttt tcagccactg cggtacatca tcaatcacct gttcttcgcg
     5761 gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact cttccagccc
     5821 tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa aagcgttatt
     5881 ggtccggtat acctgcgaag cgacaaagca ataaggcaac aacggcaggt aatgctgctc
     5941 aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaact cataaaaact
     6001 ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta acacgcgctt
     6061 accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat aatagtcgtg
     6121 ctgatgcagc ccgctgatac tctcagtttt gttatagata aacacatgga agtttttgcc
     6181 attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca ctggctgcat
     6241 catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta atcagttcac
     6301 gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga ctcactttca
     6361 tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc gcttcattca
     6421 atgccatccg tgactgatcc atcatggctt ttgctgcagc aaaatcgccc tgcttcgcct
     6481 gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga gttgatgata agccccatca
     6541 ccacttcttc cagctcttca gcttccgttt gcgtatcggg aatgttatcg agatccatca
     6601 tacatcgttc ctctctattc ttaccggcac gattacccgt accggcatcg attaaaattt
     6661 cagggcgtta gcgatatctt cttcgctctc ttctttatca atcgcgtttt gtgctttgtt
     6721 agccaccaca acaaagggca gataaattaa cgttgcgata ccaaggttga agagtgcgac
     6781 cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg ttggcatggt
     6841 ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg cgagggtgat
     6901 tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt tcatgataat
     6961 tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca gcgccagttt
     7021 tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga tcaggcctaa
     7081 agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg cccagatatg
     7141 gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat aggtcgcgat
     7201 attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg ccagcgcgcc
     7261 atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc ccaccacgct
     7321 acccaacgat gccagtgggg ttgagatgga atccataatg atctgatgga agttggtgcc
     7381 ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa agccgggaat
     7441 taatgccgag aaggaacgcg atactgaagc aggtacgctg tcgggcagtt taatgaccca
     7501 attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa taatcccgga
     7561 gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac cgacactata
     7621 tggggtgacg gtcataaatg ctgcaacgga taacaacccg gccgccagcg catcgacttt
     7681 acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca ttatccctaa
     7741 tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt caatggttga
     7801 ggcgtcgagg cgaataccta aggaataaaa aaacgacccc tccccaaagc ttagaaaaac
     7861 gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc gaataaagcc
     7921 atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag ggaggagtac
     7981 ct
//
LOCUS       DAZFFI010000169         7915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFFI010000169 DAZFFI010000000
VERSION     DAZFFI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.171"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..848
                     /gene="csgD"
                     /locus_tag="ACHJ12_004579"
     CDS             198..848
                     /gene="csgD"
                     /locus_tag="ACHJ12_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4992265.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            853..1242
                     /gene="csgE"
                     /locus_tag="ACHJ12_004580"
     CDS             853..1242
                     /gene="csgE"
                     /locus_tag="ACHJ12_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4992266.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1267..1683
                     /gene="csgF"
                     /locus_tag="ACHJ12_004581"
     CDS             1267..1683
                     /gene="csgF"
                     /locus_tag="ACHJ12_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4992267.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1710..2543
                     /gene="csgG"
                     /locus_tag="ACHJ12_004582"
     CDS             1710..2543
                     /gene="csgG"
                     /locus_tag="ACHJ12_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4992268.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2607..3098)
                     /gene="ycdZ"
                     /locus_tag="ACHJ12_004583"
     CDS             complement(2607..3098)
                     /gene="ycdZ"
                     /locus_tag="ACHJ12_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4992269.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3200..3754)
                     /gene="ycdY"
                     /locus_tag="ACHJ12_004584"
     CDS             complement(3200..3754)
                     /gene="ycdY"
                     /locus_tag="ACHJ12_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4992270.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3778..4515)
                     /gene="ycdX"
                     /locus_tag="ACHJ12_004585"
     CDS             complement(3778..4515)
                     /gene="ycdX"
                     /locus_tag="ACHJ12_004585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4992271.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4570..5508)
                     /gene="ghrA"
                     /locus_tag="ACHJ12_004586"
     CDS             complement(4570..5508)
                     /gene="ghrA"
                     /locus_tag="ACHJ12_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4992272.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5742..5829
                     /locus_tag="ACHJ12_004587"
     tRNA            5742..5829
                     /locus_tag="ACHJ12_004587"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5776..5778,aa:Ser,seq:gga)
     gene            complement(6203..7189)
                     /gene="ycdU"
                     /locus_tag="ACHJ12_004588"
     CDS             complement(6203..7189)
                     /gene="ycdU"
                     /locus_tag="ACHJ12_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO4992273.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7186..7713)
                     /locus_tag="ACHJ12_004589"
                     /pseudo
     CDS             complement(7186..7713)
                     /locus_tag="ACHJ12_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            7780..>7915
                     /locus_tag="ACHJ12_004590"
     CDS             7780..>7915
                     /locus_tag="ACHJ12_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4992274.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
BASE COUNT         2239 a         1950 c         1770 g         1956 t
ORIGIN      
        1 gttacatgtt taacacttga tttaagattt gtaatggcta gattgaaatc agatgtaatc
       61 cattagtttt atattttacc catttagggc tgatttatta ctacacacag cagtgcaaca
      121 tctgtcagta cttctggtgc ttctatttta gaggcagctg tcaggtgtgc gatcaataaa
      181 aaaagcgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat acattattgt
      241 tgatcactaa accgtctttg caagcgacag ctctcttgca gcaccttaaa caatcgctgg
      301 caattacagg aaaattacat aatattcaac gttctctgga cgatatatct tcaggctcta
      361 ttattcttct ggatatgatg gaagcagata aaaagcttat ccattattgg caggatactt
      421 tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa gattacccgt
      481 accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg gaggatcaag
      541 aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga atgctacttt acgcaaaagc
      601 ttgccagtta cctgattaca cattctggta actatcgtta taacagcacg gaatcagccc
      661 tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg tctaataacg
      721 agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt tataatcttt
      781 tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac gataacctca
      841 ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt tctgttcgcc
      901 gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgttaactga ccatactgtt
      961 tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag tgactatacg
     1021 ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat cactataacg
     1081 gtcaatcagg acgttatttt ccagactttt ttatttccgt tgaaaagaga cttcgagaaa
     1141 actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca gataaatcag
     1201 gcgttattaa gtacgggcga tttggcgcat gatgaattct aaataaaaaa ttgttcggag
     1261 gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc attaagttgg
     1321 gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc aaataatggc
     1381 gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag ctataacgat
     1441 gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat ccagtcacaa
     1501 attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat ggtgaccaac
     1561 gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt gacagatcgt
     1621 aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc aaccgatttt
     1681 taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg gttgccgtca
     1741 tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg acattaatgc
     1801 ctcgagctca gagctacaaa gatttgaccc atctgccagc gccgacgggt aagatctttg
     1861 tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg gcaagtaact
     1921 tctccactgc tgttccgcaa agcgccacgg caatgctggt cacggcattg aaagattctc
     1981 gctggtttat accgctggag cgccagggct tacaaaacct gcttaacgag cgcaagatta
     2041 ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg ctgcaatctt
     2101 taacggcggc aaatattatg gttgaaggtt cgattatcgg ttatgaaagc aacgttaaat
     2161 cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac cagctcgatc
     2221 agattgccgt gaacctgcgc gtcgtcaatg tgagtaccgg tgagatcctc tcctcggtga
     2281 acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc tttattgact
     2341 accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt atgctgtgtc
     2401 tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc gaccgtggtc
     2461 tgtgggattt gcagaacaaa gcagaacggc agaacgacat tctggtgaaa taccgccata
     2521 tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc ggcattttac
     2581 tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg ctcacggtga
     2641 gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc gtaaccaaat
     2701 accagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc ggcaaacgtc
     2761 gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca gcagctgttt ggcctgaata
     2821 cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag atgtggtgcc
     2881 agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag ggttgcagcg
     2941 gagatcgcca gccctttcag cccaccttgc gggcaggcaa agtaggccgt acagcccagg
     3001 aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat accggagaga
     3061 atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat agcagaaaac
     3121 gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct gttgcagtta
     3181 tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc cacattgcac
     3241 taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct
     3301 ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac agtgtttcca
     3361 gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca agcagcaatg
     3421 tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa agaaaagcgc
     3481 gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc acggcacatt
     3541 catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca cagcttttct
     3601 gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt ttcccctcac
     3661 gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa tagagcgaac
     3721 ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta ctagtaatta
     3781 aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt tcagtaagcg
     3841 gcgcggcgaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga ggattttaag
     3901 acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta atgccaccca
     3961 acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac tgcccttacg
     4021 tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg cggctgcttc
     4081 agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta ttatatgcac
     4141 attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt catgtggtgc
     4201 aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca ttttaccgct
     4261 gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca ggatccctac
     4321 cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat gcggagcatc
     4381 ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg
     4441 ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat gcatatgaag
     4501 gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct
     4561 gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct cccctttttc
     4621 gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc
     4681 gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg
     4741 ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca gcgccgcgag
     4801 caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatccgg
     4861 taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa
     4921 attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct
     4981 ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgccaggt
     5041 ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc cgatggtaaa
     5101 atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata
     5161 atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc
     5221 gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg
     5281 tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag
     5341 atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat cagcagagtc
     5401 attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc gcagtgcctc
     5461 aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga
     5521 aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc caccagcata
     5581 acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca cacattgatt
     5641 gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt catgatttcc
     5701 ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg tccgagtggc
     5761 tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg aatccccccc
     5821 tcaccgccat atttaaagaa gagctcgtac gaaagtacgg gctttttttt cgtatattgc
     5881 acacaccggg gggatgagaa gccccaaccg gggttcgaca actggcgaca gccagttgga
     5941 cagaccgtga acgcagtgag cgggctgccc gcagggcgag cgaagcgagt caatcccccc
     6001 ctcaccgcca tatttaaaga agagcccgta cgaaagtacg ggcttttttt tcgtatattg
     6061 cacacaccgg gtgcccccgc ccacgtctgt tagggcgagg ggaaattgtg cacatcaact
     6121 gtctggttac tcagtcaaca atcaaaatgc gcaagatagt agagaacata tatgcgttat
     6181 ctggtcactt cctgtaactt cctcacttat taaaatgccc ccaacggaaa tacatgtcac
     6241 atgccatcat aaaatcgtta tgccagagga atcgacaatg gaaaaaatag taaattaagc
     6301 acggtacata gcagagtaaa aaataggctt caaaccaaat ccgccattcc acaagcgttg
     6361 ccaataccga caccagcgta atccacatca tttcaataac cagcaatatg aataaccgaa
     6421 gccaaagcgg cttggcaaaa aaggtcgaca acgcattttc actggagaaa aacagatcga
     6481 tttcctgcaa aaaaagcctg cccagaaaag cgtaccgctt ggagaagcgc agaaaaaaca
     6541 gattaatcgc caacactacg ccacaaagaa tgagcaatgc aattatcgat ttaaacgtta
     6601 accactgccc ctctgtcaca aaagaatcac tgttacgcaa cgggctgata gtaagcaggt
     6661 taacaaccgt tgagatgcac accgaaagca gcacatagcg agtcagactt ttatatttta
     6721 cttttttcgc tactgaaata gcgacctgct tgacataact ggtgcggatc atccgaaaca
     6781 gcagaaagat gaccaacggc ccaagtaacg aaatcaggaa cagcgcatat aacgagggta
     6841 gaagctggta tagcccccaa ctcattgcac tcaacatgat taacgacaga aatcctgcca
     6901 ccagaggttg tgtaagaaat aattctattt tgtggttctg ctttttctta aaccacgaaa
     6961 aatcgatatt cttcgtgacg catttataag cccagtacgc cttgagatca aaaataaaat
     7021 tatacagcac cagggccaga atcccactaa ctacaccaat atcctctgat ggaaaagtaa
     7081 accctgcatt cttccaataa aagcctatca gaataagtcc aatagcataa atgtatatca
     7141 gatttcgttt atcctgaaag cgaataaaag ttaaataatc aggaatcatt tgaatacctt
     7201 tgcatcacta aatgctttac gacaatgcat aactcgttgc tgaaccacgc cagcaaaatc
     7261 atttaacata ttccccaaat agggcttatt acttatttca aggaaagcta ccggggttgg
     7321 catattcaga aatgtatcga aagattcagt ccatttcacc gtatgagtta agtgtaatga
     7381 gagattttct ttgataattg acggagcgac ctcgcttttt gctgttacgt tcatcagaac
     7441 ctgatgctct ggcgaagcga tatccagtcc ggcaagataa tcgcgcattg cctgaacgcc
     7501 gtcttccatc aaacgcgtgt gccaggcacc gctcacaccg agtttaaccg gttcgtatcc
     7561 agcggccatc agcagcgtgg caaattcatt caacgcagcc tgcgttcccc caataacctg
     7621 ctggcgcggc gtgttatcac agctaatatc cagcgcaatg cctgattccg taatcatcgt
     7681 ctgcagttgt tcgcggttga tgcctttaac cgcctgatct tacccagcaa tagtggacac
     7741 gcggctaagt gagtaaactc tcagtcagag gtgactcaca tgacaaaaac agtatcaacc
     7801 agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca gtgaagccct gaagcttgct
     7861 gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc tgtatgaatc acagc
//
LOCUS       DAZFFI010000170         7870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFFI010000170 DAZFFI010000000
VERSION     DAZFFI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.172"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            277..1002
                     /locus_tag="ACHJ12_004591"
     CDS             277..1002
                     /locus_tag="ACHJ12_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992275.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            960..1637
                     /gene="bglJ"
                     /locus_tag="ACHJ12_004592"
     CDS             960..1637
                     /gene="bglJ"
                     /locus_tag="ACHJ12_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4992276.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1675..2463)
                     /gene="fhuF"
                     /locus_tag="ACHJ12_004593"
     CDS             complement(1675..2463)
                     /gene="fhuF"
                     /locus_tag="ACHJ12_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4992277.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2604..2840
                     /locus_tag="ACHJ12_004594"
     CDS             2604..2840
                     /locus_tag="ACHJ12_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4992278.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2879..2965)
                     /locus_tag="ACHJ12_004595"
     tRNA            complement(2879..2965)
                     /locus_tag="ACHJ12_004595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2929..2931),aa:Leu,seq:cag)
     gene            complement(3266..4297)
                     /gene="rsmC"
                     /locus_tag="ACHJ12_004596"
     CDS             complement(3266..4297)
                     /gene="rsmC"
                     /locus_tag="ACHJ12_004596"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4992279.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4400..4813
                     /gene="holD"
                     /locus_tag="ACHJ12_004597"
     CDS             4400..4813
                     /gene="holD"
                     /locus_tag="ACHJ12_004597"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4992280.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            4782..5228
                     /gene="rimI"
                     /locus_tag="ACHJ12_004598"
     CDS             4782..5228
                     /gene="rimI"
                     /locus_tag="ACHJ12_004598"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4992281.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5243..5920
                     /gene="yjjG"
                     /locus_tag="ACHJ12_004599"
     CDS             5243..5920
                     /gene="yjjG"
                     /locus_tag="ACHJ12_004599"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4992282.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6306..7541
                     /locus_tag="ACHJ12_004600"
     CDS             6306..7541
                     /locus_tag="ACHJ12_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4992283.1"
                     /translation="MALSDAWLRSVVGKKRDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELELNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
BASE COUNT         2032 a         1812 c         1991 g         2035 t
ORIGIN      
        1 ataaattaaa tgaatgtgat ttctgttttt cattaagtat tcctaacaac ttaatgtaga
       61 tattttaaat gtctccaggc tatttcacca ggttatcatt tgatgctaac cttctgacac
      121 atgcagtgga gttgttgtgc agcaggagta tgctgatatg aaagtgaatg ctaaggataa
      181 tttattcgct taatctatta atttgctgga aacatttaag gaatgaatat cctgaataaa
      241 ccatcataat ttttagacag tggatgtgga ggaaatatgt tgccaggatg ctgcaaaaat
      301 ggaattgtta tcagtaaaat acctgttatg caagcagggt taaaagaggt catgaggact
      361 cacttccctg aatatgaaat aatatccagc gcctctgcgg aggaccttac cttattacaa
      421 ttacgtcgtt ccggattagt cattgctgat ttagccggtg aaagtgaaga tccacgttct
      481 gtttgtgaac attattattc tttaatctca caatatcggg aaattcactg ggttttcatg
      541 gtgtcacgct cctggtattc ccaggcagta gaattgctca tgtgccctac ggcgacgtta
      601 ttgtctgatg ttgaacccat tgagaatctg gtcaagaccg tacgttccgg caatacgcac
      661 gcagagcgta tcagcgccat gctgacctct ccggcaatga ctgaaactca tgattttagc
      721 tatcgctccg tcattctcac tctttcagag cgcaaggtac tgcggctatt aggtaaagga
      781 tggggcatca accagatagc ttcgttgctt aagaaaagta ataaaactat cagcgcccaa
      841 aaaaacagtg cgatgcgtcg actggcaatt cacagcaacg ctgaaatgta tgcatggata
      901 aatagcgcgc agggtgcaag agaacttaac ttgccttctg tttatggaga tgccgcagaa
      961 tggaacacag ccgaattaag aagagaaatg tcgcactcat agaaaaatgc gtcatgagca
     1021 gtatcggtat tgagagttta ttcagaaagt ttgcgggtaa cccttataag ctccatacct
     1081 ataccagtca ggagtcattt caggatgcca tgtcgcggat ctcatttgcg gcggtcattt
     1141 tttctttttc tgccatgaga agtgagcgca gagagggatt atcttgcctg actgaactgg
     1201 cgattaagtt tccgcgtacc cggcgtttag ttattgcgga tgatgatatt gaagctcggc
     1261 tgattggttc attgtcgcca tcaccgctgg acggtgtatt aagtaaagcg tcaacgctgg
     1321 agatttttca tcaggaactc tttttgtcat taaatggtgt acgtcaggcg accgaccgac
     1381 tgaacaatca gtggtacatt aaccaaagcc ggacgctaag cccgacggag agagaaatat
     1441 tgcgctttat gtcgcgtggc tactcaatga cacaaattgc cgagcagctt aaacgcaata
     1501 tcaaaacgat ccgtgcacat aaatttaatg tgatgtcgaa actgggcgtc agttctgatg
     1561 cagggttgtt ggaggccgca gatattctgt tatgtatgcg gcattgcgaa gcaagtaatg
     1621 tgttgcatcc ctattaatcc gcatgatgcc gggtttactt ccccggcagt gctttcattt
     1681 cagcgtacaa tcgccacatt gctgcacatc cggtaagcga taacgctggc agcaggtccg
     1741 gcgcaccagc aggccgtcgc gtagcaccac ggtacgccag agtggattat cttcaccggt
     1801 agtaagcgtt ttctcaaaaa agagggcatg gcgcagcgat tcaaccgtcg cctcgccgag
     1861 cagttgcttc atctcagtaa gataccagtt gatcaaataa ccggtattac tccagataag
     1921 tttgccgttg atctctccgg tcgcttctag tgcctgcaca accggaacca gcgcctggct
     1981 gattaacctt tccattcgct gctgcggcga atgtggtgtt gcgtttttat cttcacacac
     2041 atcgacccag aaacaggcgg cacggccggt ttcgtgaaac tcagcatgga aatgttccgg
     2101 cgacacatct aatgcctttt cctgcgtcag tagcgccagc attaatggtg gcaccatcag
     2161 gccgatatac cattgtgccc atagcgagat cagcggtttg ttctcgcgga tcatcgttgg
     2221 ttggttgcga tagatatgat cggaatagac cgcgagtaga gaacttagcg cattcggtga
     2281 tgaccattgc gccagcgtca tggcgttaag cggagcgggt tcatccaggc ggataaactc
     2341 cagcaaatgt tcacgatgtt tggcgatcgt cgcacgtacg gcttgtgcaa gcccagcatc
     2401 ctgtggctgg agatgcgttc gccagataac atcttcatag agcggtgcgg aacgataggc
     2461 cataatcggg atagtaatct aaatgataat gattgctaat cctagcgata ggtttatccg
     2521 atagcaaggg atttctctgg cttgcaaatg ataaaaatta tcatatgata ttggttatca
     2581 ttatcaatga aagagatgaa accatgttgc aacgtacgct gggcagtggc tggggagtgt
     2641 tgctgccagg attgctgatt gcagggctga tgtatgcgga tttatcgcct gatcaatggc
     2701 ggattgtcat tctgatggga ttagtattga cgccggtgat gctgtatcac aaacagttac
     2761 ggcattacgt tttgctacca tcgtgcctgg cgcttattgc tggcatcatg ctgatgataa
     2821 tgaatttgaa tcagggatga aaaatcaagg aagaaacaag aaaggaagta aagataattg
     2881 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
     2941 cgtctaccaa ttccgccacc ttcgcatacc atcaattctt aaaaagaatt gctaccacgg
     3001 aggcgcattc tagtggtttt cagcttttcg tcaatagtta attatcgccg gaggtgtaat
     3061 tgctggaaaa atgtccatca ggaaactagc gtgcaggttt tgtaggtcgg ataaggcgtt
     3121 cacgccgcat ccgacacggt atttgggtgc agatgccaga tgcgacgcgg gcgcgtctta
     3181 tctggcctac gaagggctaa cgtgcaggtt ttgtaggtcg gataaggcgt ttacgccgca
     3241 tccgacacag tattcggcgg gatacttaac ctttcttcgc ctggcgggtc ataatggcgc
     3301 gatacacctt gaagcgcccg gtttgcgcga taacttcatg gaagccaaat gtctcatcca
     3361 gcacgtccgg gtaaggcagg aaggcgttcg ctacaattcg cagctcgccg ccgctattaa
     3421 gatgacgcac cgcgccgcga atcagcgttt gcgccgcatc caggctggtt tgcatcccat
     3481 cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg gaaaagacgt
     3541 tgctggcaaa gacttcacct tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta
     3601 ccgctggcgc agagacatcg cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg
     3661 caactgaaag cacccccgcg ccacagccga catccagcac tttacctttc gtgtgcggag
     3721 ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta aacacgccag
     3781 gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa
     3841 ataccggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt
     3901 tattcaacgg cgcataatct gccagcatct gctcggcgct gcgcacgccg ctgcggttct
     3961 cgccaacgac aaaaatatcc gtccccaccg gcagcagaga aagtaaattc atcaactgga
     4021 actgggcttc cggtttgttc ttcggccagt agtaaatcag cgtatcgcaa tctgcgacgt
     4081 catccgccgt ggcgaccaga ctgaaacggg cgttatcccc catctggcgg cttaatacct
     4141 gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca
     4201 ggtcatcctg taagtctccg gcaaacagaa tacggctttg ttcgaaatca tcactgtgac
     4261 gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca attgatactg
     4321 gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg
     4381 acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc aactgggcat
     4441 tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca ttgcgatccc
     4501 ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgactg atcctttagt
     4561 gagcgatgtt ctgcgcgcat taaccgtcag ccccgaccag gtgctgcaac tgacgccaga
     4621 aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg gtactgacga
     4681 accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgatt tacgggcaaa
     4741 cccaacggca cgcgccgcgt tatggcaaca aatttgcaca tatgaacacg atttcttccc
     4801 tcgaaacgac tgatttaccg gcggcttacc acattgaaca acgcgcccac gcctttccgt
     4861 ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt cagttaacgc
     4921 aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa gctacattgt
     4981 tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggcg ctgctggaac
     5041 atctgatcga cgaactggaa aaacgcggcg tggcgacact atggctggaa gtccgtgctt
     5101 caaacgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc
     5161 gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg ttgccaatca
     5221 gtatgtaaga caaggtggaa taatgaagtg ggactggatt ttctttgatg ccgatgaaac
     5281 gctgtttacc tttgactcat tcaccggcct gcagcggatg tttcttgatt acagcgtcac
     5341 ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt gggtggatta
     5401 tcaaaacggc gcgatcactt cattacagct tcagcacggg cgttttgaga gctgggccga
     5461 acggctgaaa gttgaagcag gcttgcttaa cgatgccttt attaatgcga tggcggaaat
     5521 ctgcacgccg ctgccgggcg cggtttctct gcttaacgcc attcgtggca acgccaaaat
     5581 cggcatcatc accaacggct ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct
     5641 gcgtgattac ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa
     5701 gaaaattttc gattatgcgc tggaacaggc gggcaatcct gaccgttcac gcgtgctgat
     5761 ggttggcgac actgccgagt ccgatattct cggtggcatc aacgccgggc ttgcgacctg
     5821 ctggctgaat gcgcaccatc gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc
     5881 ttcgttgcac gaactggagc agctcctgtg taaacactga ttgcctcccc cccgttgatg
     5941 ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga
     6001 agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc
     6061 cattatttct caccccaata attaagccta aattaaagct cttttacttt ttcaaagtcc
     6121 tttcagttaa ttgatgtttt gttttataag tcatttaaat acagttagtt atgtggcttc
     6181 tttaattgaa aattctttca tgtaacttta aatcgatcag gttgctttca tcaaaaatct
     6241 gtacatatgc ttgtacataa tgtacaaagc aacagaggtg ttttgcgatt tgtacaaggt
     6301 gagtaatggc gctgtctgat gcgtggttgc gttcagtcgt tggaaagaaa cgtgataagg
     6361 ttttggttaa atccgatcgt gatggtctgt ctgtcagagt atcaccgaaa ggtcgcgtag
     6421 tgttccaata tcgttatcaa tgggcaggga aaggtgagcg ccttgatatc ggaacttacc
     6481 cggcaactgg attaaaagag gccagagaag aagttatccg tctccgtggt gaactcgagt
     6541 taaaccgcaa tccacgatta gtcaagcagg ctgaaaaacg aaaagctact gaagccatga
     6601 cggtagagtc tgtgatccgt gcctggtatg aagcatattg tgtaaaaaat aaaaaaggtt
     6661 ctgaacagat actccgctcg tttgagctgc acctgttctc taaaatcggg aatatccctc
     6721 acgatgcagc tacattgcat gattggttag aagtcctgga gcctcttagc accaagactc
     6781 cagcaatagt agaccgattg ctaattaacg caaagcaggc tcatgtctgg gcgtataaga
     6841 gaaagctcat tgaaactcgc ccactgtcgg atatcacggg taaagatatg gatatccgta
     6901 aaggtcagaa aaaacggttt ctgacacatg atgaaattaa catcctttat gctgcgatcg
     6961 atggttctcg aatggttcct aaataccgag ccttcattaa actattgctg cattttggtt
     7021 gccgtagttc agagctaatt actgctaggg tggatgattt tgatttcata aataaggtat
     7081 ggactgtacc accagaacga cataagactg gggacataac aggcgaaccg ctaaagcggc
     7141 ccattattga accggttgaa gagcttataa aatacgttat ctctatgaac aacggttccg
     7201 atatgctttt taccaaggaa ggaagcaggg agccagttgg tcggacatca ttgcagtcgc
     7261 tgccttacaa tttaatgcag ttcgcatggc gacgtttggg gtatcaattt cctcattggt
     7321 ctcttcatga tttgagacga acagcacgaa caaacttttc tgatcttact gctcctcata
     7381 ttgctgaaat aatgctcggt cataaactgc caggtgtatg gcaagtttat gataagagcg
     7441 attatctaga agaacagcgt aaagcttact gggcatggtg ggagagggtt gaatcgatcg
     7501 ttacttgtac tgacttaatc tccaacggac agtttgcgta gccagagcgt aatcatatct
     7561 gccagtccgc tgcggatacc ttgtgtactg ggtttttagc cagactacgt taaccggtag
     7621 cttcagtatt tgggggggcg gcaggagttt gatggtttat tgggttactg tgggactaag
     7681 gatgtaggga tgccaatcaa tgaattatga aaaatatcat agcacaaaca gaagcaaggg
     7741 tttgcgaacc ttctgctact taatttatta tgtgcaggtg atttttaatg tgatgaagtg
     7801 ttttatgact cgcggaaact ttgattttat aaactctaac gtggttttct cagaagccga
     7861 taagtcattg
//
LOCUS       DAZFFI010000171         7848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFFI010000171 DAZFFI010000000
VERSION     DAZFFI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.173"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..683
                     /locus_tag="ACHJ12_004601"
     CDS             <1..683
                     /locus_tag="ACHJ12_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO4992284.1"
                     /translation="DEDLNDRDFIASVVDRAIFHFAINSICNPGANKDATPIERCTFD
                     VETKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLL
                     ISLSYSSAYTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEMKHLADRGGMKF
                     DDYLDKMSLLVNEGKLEPDILSKMRDAAPKLIDFAKSFDPNSKEKIKILTDTSNLIYD
                     LFGVKSAK"
     gene            1047..2084
                     /gene="dusA"
                     /locus_tag="ACHJ12_004602"
     CDS             1047..2084
                     /gene="dusA"
                     /locus_tag="ACHJ12_004602"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4992285.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2218..2460
                     /gene="pspG"
                     /locus_tag="ACHJ12_004603"
     CDS             2218..2460
                     /gene="pspG"
                     /locus_tag="ACHJ12_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4992286.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2626..3609)
                     /locus_tag="ACHJ12_004604"
     CDS             complement(2626..3609)
                     /locus_tag="ACHJ12_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4992287.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGCKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3692..5107
                     /gene="dnaB"
                     /locus_tag="ACHJ12_004605"
     CDS             3692..5107
                     /gene="dnaB"
                     /locus_tag="ACHJ12_004605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4992288.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5160..6239
                     /gene="alr"
                     /locus_tag="ACHJ12_004606"
     CDS             5160..6239
                     /gene="alr"
                     /locus_tag="ACHJ12_004606"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4992289.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6492..7685
                     /gene="tyrB"
                     /locus_tag="ACHJ12_004607"
     CDS             6492..7685
                     /gene="tyrB"
                     /locus_tag="ACHJ12_004607"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4992290.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1904 a         1873 c         2045 g         2026 t
ORIGIN      
        1 ctgatgagga tttaaacgat cgtgacttta ttgcatcagt tgttgaccgg gctatttttc
       61 atttcgcgat taatagtata tgtaatcctg gggctaataa agatgcgact cccattgaac
      121 gatgtacttt tgatgtagaa actaagaatg accttccctc cacggttcag ctattttatg
      181 aggaatctaa ggataatgaa cctttagcga atatacattt tcaagcaata ggttctggtt
      241 ttttaacgtt tgttaatgcc tgccaggaac atgatgacaa cagcttaaaa ttatttgctt
      301 cgctgttaat ttcactttca tattctagtg cctacacaga tttagccgga gcagaaaaag
      361 tgaatattaa tgaatataat gagaactatc tgacagctca gtttgaagaa ttatctcaac
      421 gtgatatgaa gaagtacctg ggagagatga agcatctggc ggaccggggg gggatgaaat
      481 ttgatgacta tctggataaa atgtcacttc tggtgaatga aggaaagctc gaacctgata
      541 ttttaagcaa aatgcgagat gctgcaccga aattaattga ctttgctaag tcgtttgacc
      601 caaactcaaa ggaaaagatt aaaatactta cagatacttc taatttaatt tatgatttat
      661 tcggggttaa atcggcgaaa taatatgtga agttcttcga tggtatggaa ggcattacat
      721 aaaagaacct aatacttatt gggttctttt ttcttctatc agtgccatta gcaggaagag
      781 atatcaccgg agtttaatgt gtgatttttt atttatcgtc gaacctggat tgtttatcat
      841 tgtccttaac aaggctaacg gctaataaga ttatttccat cacttcgtga gagcttcatg
      901 ccttgatttg atctcaattt tcttttgcaa tgagacaggc gcttcctgtt gttatggtat
      961 agtaccccgc tattgagcct cctgaacagt gatgctgaat aacataaccc aatgatatat
     1021 cgataaaata atctctacat ttgaaaatgc acggtaattc tgaaatgcaa aaaatcaacc
     1081 aaaccagcgc aatgcctgaa aaaactgacg ttcactggag tggtcggttt agcgttgcac
     1141 caatgctcga ctggacggac agacattgcc gctatttctt gcgtctgctt tcccgcaata
     1201 cgttgctgta taccgaaatg gtgaccacag gggcgattat tcacggtaaa ggtgattacc
     1261 tggcgtacag tgaagaagaa catccggtag cgttgcaact cggcggtagc gatccggcgg
     1321 cgctggcgca gtgtgcaaag ctggcagaag cgcgcggata tgatgagatc aacctgaatg
     1381 tcggctgccc gtctgaccgg gtgcagaacg gcatgtttgg tgcgtgtctg atgggtaatg
     1441 cgcagctggt tgccgactgc gtgaaagcga tgcgcgatgt ggtgtcgatt ccagtgacgg
     1501 tgaaaacgcg tattggcatc gatgaccagg acagctatga atttctctgc gatttcatca
     1561 acaccgtttc cggcaaaggc gagtgtgaga tgtttatcat ccacgcacgt aaagcctggc
     1621 tttcggggtt aagcccgaaa gaaaaccgtg aaatcccgcc gctcgattat ccgcgtgtgt
     1681 atcaactgaa gcgtgacttt ccgcatctga caatgtcgat taacggtggt atcaagtcgc
     1741 tggaagaggc caaagcacac ctgcaacata tggatggcgt gatggtcggg cgcgaggcgt
     1801 atcagaatcc gggtattctg gcggcggtag accgggagat ctttggttcc tcggataccg
     1861 atgccgatcc ggtggcggta gtgcgcgcca tgtatccgta cattgagcgt gaactcagcc
     1921 aggggacgta tctcggccat attacccggc atatgttggg cttgttccag ggtattcctg
     1981 gcgcgcggca gtggcggcgt tatttaagtg aaaatgccca taaagcgggt gcagacatta
     2041 atgtgctgga acacgcgctc aaactggtgg cggataagcg ttaacttttc accaaaaagt
     2101 agtcaaattc accacgccct gcgcaccgtc gcggggcgtt ttgctgttaa atcaatagat
     2161 tatttttggc atgattcttg taatgccagc aagagatttc atatttggga gagcatcatg
     2221 ctggaactac tttttgtgtt tggctttttt gtcatgctga tggtcaccgg cgtttcgttg
     2281 ctgggcatta tcgccgcgct ggttgtggcg acggccatta tgttcctcgg cggtatgctg
     2341 gcattgatga ttaagttgct gccgtggtta ctactggcga ttgcggtggt gtgggttatt
     2401 aaggcgatta aagcaccaaa agtgccgaaa tatcagcgtt atgaccgctg gcgttactaa
     2461 gggattgtgc ggatgatcac aacctaaggt tttatcctta gaacaaaata ggaattgata
     2521 atcaaatctg tcactattgc gcctctaaca gattcatcgt gctgtaccct acatacagcc
     2581 gaactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg gaatcagcag
     2641 gctggaacct tgcgtcgccc ggctttccag aatctcatgc gcacgctgcg catccttcag
     2701 cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa tcaaagagaa
     2761 cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt gcagggaagg
     2821 gcgtgtcaca tacaacgagc ctttttgatt gagaatgcct aagttcacac cggtaaccgc
     2881 acctgatgag ttgccaaaac tgaccattaa gccgcggcgt tgcaggcaat ccagcgaccg
     2941 ttcccaggtg tctctgccca cggaatcgta cactacgcgc actttcttac agccggtgat
     3001 ctctttcaat cgctcgacca gattctcttc acgatagtta ataacctgcc acgcgccggc
     3061 ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt tcgcgcccag
     3121 ggcttttgcc cactggcagg caattaagcc aacgccgcca gccgctgcgt ggaacaggaa
     3181 ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg ttaagccttt
     3241 caggaaagat gctgcagctt gttcaaaaga aattgccgca ggcagaatcg ccgctttatc
     3301 cgcattaatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat agactacacg
     3361 atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc ctgccgcttc
     3421 ggtacctaat ccgctgggta gtgatggcgg cgggtaaagg ccgctgcgga tatacgtgtc
     3481 gataaaattg atgccgatgg ctttattttc gacctggatt tcattctctg ccggatcggc
     3541 aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa attcaattcg
     3601 tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt aaagtttcga
     3661 cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct tcaacaaaca
     3721 gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc ctccgcactc
     3781 gatcgaagcg gagcagtcgg tgttgggcgg tttaatgctg gataacgaac gctgggacga
     3841 tgtagccgag cgtgtggtgg cagacgattt ttacacccgc ccacaccgcc atatctttac
     3901 tgaaatggcg cgtttgcagg aaagcggtag tcctatcgat ctgattaccc ttgcggaatc
     3961 gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg cagagctgtc
     4021 aaaaaatacg ccaagtgcgg cgaacatcag tgcctatgcg gacatcgtgc gtgaacgtgc
     4081 cgttgtccgt gagatgatct cggttgcgaa tgagattgct gaagccggtt ttgatccgca
     4141 ggggcgtacc agcgaagatc tgctggacct tgctgaatcc cgcgtcttta aaattgccga
     4201 aagtcgtgca aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg acgcaaccgt
     4261 ggcgcgtatt gagcagttgt ttcagcagcc acacgatggc gttaccggag taaacaccgg
     4321 ttatgacgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga tcatcgtcgc
     4381 cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa acgcggcgat
     4441 gttgcaggat aaaccggtac ttatcttctc gctggagatg ccttcagaac agattatgat
     4501 gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg ggcagctcga
     4561 tgatgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa aacgcaatat
     4621 ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg cacgccgtat
     4681 tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac tgatgcgcgt
     4741 accggcgctt tccgataacc gtacgctgga aattgcagaa atctcccgct cgctgaaagc
     4801 actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc gttctctgga
     4861 acaacgtgcc gacaaacgcc cggtcaactc cgacctgcgt gaatctggct ctatcgagca
     4921 ggatgcggac ttgatcatgt ttatctatcg tgatgaggtg tatcacgaaa acagtgattt
     4981 aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg ggacggtacg
     5041 cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc agtacgacga
     5101 cgaataataa ttattttatg aattaggtaa ttaaagcaaa cacttatcaa ggaacacaaa
     5161 tgcaagcggc aactgttgtg attaaccgcc gcgctctgcg acacaacctg caacgtcttc
     5221 gtgaactggc ccctgccagt aaaatggttg cggtggtgaa agcgaacgct tatggtcacg
     5281 gtcttcttga gaccgcgcga acgctccccg atgctgacgc ctttggcgta gcccgtctcg
     5341 aagaagctct gcgactgcgt gcggggggaa tcaccaaacc tgtactgtta ctcgaaggct
     5401 tttttgatgc cagagatctg ccgacgattt ctgcgcaaca ttttcatacc gccgtgcata
     5461 acgaagaaca gctggctgcg ctggaagagg ctagcctgga cgagccggtt accgtctgga
     5521 tgaaactcga taccggtatg caccgtctgg gcgtaaggcc ggaacaggct gaggcgtttt
     5581 atcatcgcct gacccagtgc aaaaacgttc gtcagccggt gaatatcgtc agccattttg
     5641 cgcgcgcgga tgaaccaaaa tgtggcgcaa ccgagaaaca actcgctatc tttaatacct
     5701 tttgcgaagg caaacctggt caacgttcca ttgccgcatc gggtggcatt ctgctgtggc
     5761 cacagtcgca ttttgactgg gtgcgcccgg gcatcattct ttatggcgtc tcgccgctgg
     5821 aagatcgctc caccggtgcc gattttggct gtcagccagt gatgtcacta acctccagcc
     5881 tgattgccgt gcgtgagcat aaagccggag aacctgttgg ttatggtgga acctgggtaa
     5941 gcgaacgtga tacccgtctt ggcgtagtcg cgatgggcta tggcgatggt tatccgcgcg
     6001 ccgcgccgtc cggtacgcca gtgctggtga acggtcgcga agtaccgatt gtcgggcgcg
     6061 tggcgatgga tatgatctgc gtagacttag gtccacaagc gcaggacaaa gccggggatc
     6121 cggtcatttt atggggcgaa ggtttgcccg tagaacgtat cgctgaaatg acgaaagtaa
     6181 gcgcttacga acttattacg cgcctgactt caagggtcgc gatgaaatac gtggattaat
     6241 cgttctgtaa tatttgattg tctgtgccgg atgcggcgtg aatgccttat ccggccaata
     6301 aaatcctaaa aattcaataa gttgatgttc tttcatgctc ttataaaggt cgtgcctctg
     6361 gcggatgtac gtttgtcatg agtctcactc tgttgctaat tgccgttcgc tcctgaacat
     6421 ccactcgatc ttcgccttct tccggtttat tgtgttttaa ccacctgccc gtaaacctgg
     6481 agaaccatcg cgtgtttcaa aaagttgacg cctacgctgg cgacccgatt cttacgctta
     6541 tggagcgttt taaagaagac cctcgcagcg acaaagtgaa tttaagtatc ggtctgtact
     6601 acaacgaaga cggaattatt ccacaactgc aagccgtggc ggaggcggaa gcgcgcctga
     6661 atgcgcagcc tcatggcgct tcgctttatt taccgatgga agggcttaac agctatcgcc
     6721 atgccattgc gccgctgctg tttggtgcgg accatccagt actgcaacaa cagcgcgtag
     6781 caaccattca aacccttggc ggctcagggg cattgaaagt gggcgcggat ttcctgaaac
     6841 gctacttccc ggaatcaggc gtctgggtca gcgatcctac ctgggaaaac cacgtagcaa
     6901 tattcgccgg ggctggattc gaagtgagta cttacccctg gtatgacgaa gcgactaacg
     6961 gcgtgcgctt taatgacctg ttggcgacgc tgaaaacatt acctgcccgc agtattgtgt
     7021 tgctgcatcc atgttgccac aacccaacgg gtgccgatct cactaatgat cagtgggatg
     7081 cggtgattga aattctcaaa gcccgcgagc ttattccatt cctcgatatt gcctatcaag
     7141 gatttggtgc cggtatggaa gaggatgcct acgctattcg cgccattgcc agcgctggat
     7201 tacccgctct ggtgagcaat tcgttctcga aaattttctc cctttacggc gagcgcgtcg
     7261 gcggactttc tgttctgtgt gaagatgccg aagccgctgg ccgcgtactg gggcaattga
     7321 aagcaacagt tcgccgcaac tactccagcc cgccgaattt tggtgcgcag gtggtggctg
     7381 cagtgctgaa tgacgaggca ttgaaagcca gctggctggc ggaagtagaa gagatgcgta
     7441 ctcgcattct ggcaatgcgt caggaattgg tgaaggtatt aagcacagag atgccagaac
     7501 gcaatttcga ttatctgctt aatcagcgcg gcatgttcag ttataccggt ttaagtgccg
     7561 ctcaggttga ccgactacgt gaagaatttg gtgtctatct catcgccagc ggtcgcatgt
     7621 gtgtcgccgg gttaaatacg gcaaatgtac aacgtgtggc aaaggcgttt gctgcggtga
     7681 tgtaatgcag gaaagcaggc tggagctacc cagcctgcag tgaaattaaa ctgtcgtcgc
     7741 tttcactctt tctttataga tgattttttt gatgccatcg ttctacgtga gagataataa
     7801 acgttattag ttcttttatt gttaagctta tcctaattat ctggaatt
//
LOCUS       DAZFFI010000172         7827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFFI010000172 DAZFFI010000000
VERSION     DAZFFI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.174"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..822
                     /locus_tag="ACHJ12_004608"
     CDS             241..822
                     /locus_tag="ACHJ12_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4992291.1"
                     /translation="MVSLVKNQTVSLSKESPALSQLHFGLGWDPVKKKGFLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLESTCKAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELACYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            822..1979
                     /locus_tag="ACHJ12_004609"
     CDS             822..1979
                     /locus_tag="ACHJ12_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4992292.1"
                     /translation="MNLMPGGNAPVPAQELRVRITSGVQVDASAFRLYAAGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGCVELSGRQEAALILGEFYRRNNGWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVPPLPAETKISLSKVSLTKEMPAISLTKRDNFGEIRINLNWH
                     RGSGKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIYVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            2002..2457
                     /locus_tag="ACHJ12_004610"
     CDS             2002..2457
                     /locus_tag="ACHJ12_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4992293.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFEPAEFGL"
     gene            2476..3516
                     /gene="terC"
                     /locus_tag="ACHJ12_004611"
     CDS             2476..3516
                     /gene="terC"
                     /locus_tag="ACHJ12_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4992294.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPVWPKLRGHAFLLNQKEVDAELAKPENSDVTIDRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLIHLEKAVIVLLFFIAVKLGLNATDHIWHHGYSIAATTSLYVVLGVLVLGILASVI
                     FPGKPESEEKGN"
     gene            3565..4143
                     /gene="terD"
                     /locus_tag="ACHJ12_004612"
     CDS             3565..4143
                     /gene="terD"
                     /locus_tag="ACHJ12_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4992295.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLTANGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDTVPSDI
                     DRIIFVVTIHDAQARRQNFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            4211..4786
                     /locus_tag="ACHJ12_004613"
     CDS             4211..4786
                     /locus_tag="ACHJ12_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4992296.1"
                     /translation="MAVSLVKGGNISLTKEAPTMNVAMVGLGWDARVTDGQAFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQIKIDLAKVSVDV
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGAEIARFDLSEDASTETAMVFGEL
                     YRHGAEWKFKAVGQGFSGGLSALASQHGVNV"
     gene            4900..6194
                     /locus_tag="ACHJ12_004614"
                     /pseudo
     CDS             4900..6194
                     /locus_tag="ACHJ12_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6243..7451
                     /locus_tag="ACHJ12_004615"
     CDS             6243..7451
                     /locus_tag="ACHJ12_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HFO4992297.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMT
                     HHLGYEKNQSKPGTNSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFLTDDSVKKVVWLAIQSASQKWTMPLKDWRMAMSRFII
                     EFGDRLDGHF"
     gene            complement(7641..>7827)
                     /locus_tag="ACHJ12_004616"
     CDS             complement(7641..>7827)
                     /locus_tag="ACHJ12_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO4992298.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWP
                     SNRVDELLPWNVVLTNK"
BASE COUNT         1928 a         1873 c         2101 g         1925 t
ORIGIN      
        1 atatgattaa taacacctga ggttatggag tgtatcagtg tttttgtgtt acctgttaat
       61 gtgttgatat ttaattggta tttagatgaa aggacatcat gaaaaggatt tctctttgtg
      121 tttttacaga aagctaccaa atttcccttg tcagagaact tcatcatgat atgattttct
      181 tcgccagtta ctaaatatac ttttgattgt ctcagttatc agcattttaa ggaataaaac
      241 atggtttcat tagttaagaa tcagacggta tcactcagca aagaatcacc tgcattaagc
      301 cagcttcatt tcggtctcgg ttgggatccg gttaagaaaa aaggttttct tggaggattg
      361 tttggaggta acgactcaat cgatctcgat gctggctgtg tattaatgga cagcaccggt
      421 aaaacaattg acaccatctg gttccgcaaa ctggaatcca cctgcaaagc tgttgtgcat
      481 agcggtgata acctgactgg tgagggtgac ggcgacgatg aagtgattaa ggtcaacctc
      541 tcccgtctgc ctgcaaacgt tgaatacctg gcgtttacgg ttaacagctt ccgtgggcag
      601 tcgttcaatg acgtcgaaaa tgcgttctgt cgtgtggtcg atcaaaccgg caaagagctg
      661 gcttgctaca agctgactga gcagggctca cataccggga tcgttatttc ttcgctgcgc
      721 cgtaataacg gcaactggga ctttaccgcc cttggtcatg cctgtcgcgg tcgcacaatt
      781 gacgatatgc actccgatat cgtttctgcg gttattcgct aatgaacctg atgccggggg
      841 gcaatgcccc cgttccagcc caggagttgc gggtccggat aacctcgggc gtccaggtgg
      901 atgcttcagc gtttcgcctg tatgccgccg ggaaggtgca gggggatgcg gacatggtgt
      961 tttatggtca gccacgtaac gatgatggta ctgtcagcct ggtcagtgaa ggtcagtact
     1021 caacctttac tgtcgcgctc aaccggctga aacctgatgt ccagaaaatt gcgttcaccg
     1081 tgacctgtga cggtgggcag acggtgtccg gtcttcgtaa tctttccatc gacgtggagc
     1141 aaggggctac cggcctggtg agcggctgtg tcgaactgag cggccgtcag gaagcagcct
     1201 taattctggg tgaattttac cgtcgtaata atggctggaa attccgcttc gttgcgcagg
     1261 ggttcaacgg tggacttaaa ccgctggcag agcatttcgg cgtaaatatt gccgacgaac
     1321 cggctcccgc agccccgact cctccggttg taccccctct gccagctgag acaaaaatca
     1381 gcctgagtaa agtatctctg acaaaagaga tgcctgcaat cagtctgacc aaacgggata
     1441 acttcggtga aatcaggatc aacctgaact ggcaccgggg cagcggcaaa tccggctttg
     1501 caggcatgtt cggctccaaa ggcatcgacc tggatctggg agcctttgtt gagcttcagg
     1561 acgggtataa gtcggtcatt caggcgctcg gtaatgcatt cggtgattat cgcgatgaac
     1621 cttatgttca gctcaagggc gatgaccgga cgggtgatgt atcagacggc gagtggctgc
     1681 atatcaatgg acgtgaatgg aagcatatcc gcgaagtgct gatttacgcc tttatttatg
     1741 aaggtgttcc cagttgggac aagactgatg gagtggtcac catttatgtg ccggatcaac
     1801 cgcccattga gacccgcctg actgaaggtg aaaaccgtcg cacattgtgc gccattgcca
     1861 gactcgtaaa cgaaaacggt gcgattaaag ttgagcgtat caaccagtac ttcaaaggcc
     1921 aggacgaaat ggaccgggca tttggctggg gatttcgctg gagcgccggt tctaaataac
     1981 acagcaataa aggaaactgg tatgagcttt ttcgacaaag ttaaaggtgc cattaactcg
     2041 ggtcgtgacg aactgacccg ccaggttggt cgtttcaaaa acaaaaaatt catgcagggc
     2101 accgttgctg tatgtgcccg tattgccgta tcgagtgacg gcgtgagttc ggaagaaaag
     2161 cagaaaatga tgggctttct gcgctcttca gaagagctga aggtcttcga taccaatgag
     2221 gtgatcgagt tcttcaataa actggtttca agcttcgatt tcgatgttga aatcggcaag
     2281 ggcgagacca tgaaatacat cctggcgctg aaagatcagc ctgaggccgc tcaactggcc
     2341 ttacgtgttg gtattgctgt tgcgaaaagc gacggtaact tcgatcagga cgagaaactg
     2401 gcctcccgcg agatcgctat cgcgttgggc ttcgagccgg ctgaatttgg cctctgacat
     2461 ccctaagggt aaaatatggt ttccacacac atcggcttcc cgactgaaac ggtcattgtt
     2521 tttattgctc tttcagtcgg agccatcttt attgacctgt ttatgcaccg tgatgacaag
     2581 ccaatctccc tgaagagtgc ggcgctctgg tccgtattct gggttgttgt tgcgatggca
     2641 tttgccggtt tcctctatat ccaccacggt gctgaagttg ccagtttgtt tgtcacgggg
     2701 tatgcgctgg agaaagtact gtcggtcgac aacctgtttg tcatgatggc catcttttcc
     2761 tggttcgctg ttccggatcg ttatcggcac cgtgtgcttt actgggggat cattggagcg
     2821 atcgtcttca ggggaatctt tgtcgccatc ggtacgagcc tgctgagtct ggggccgtac
     2881 gttgaagtcg tcttcgctat catcgttgcc tggacagcgg tcatgatgct taaaagcggt
     2941 gatgatgatg atgaaattga agattactcc cagcatctgg cttaccgcat ggttaaacgt
     3001 ttcttccctg tctggccgaa gctacgtggg catgccttcc tgcttaacca gaaggaagtg
     3061 gatgctgaac tggcgaaacc agaaaacagt gatgtaacca ttgaccgtgg taaaaaggcg
     3121 gcgctgtatg cgacccctct gttcctgtgt gtggccgtgg ttgaactctc ggatgttatg
     3181 tttgcgtttg actcggtacc ggcaatcatt gctgtcagtc gtgaaccgct tatcgtctat
     3241 agtgccatga tgtttgctat cctggggctg cgtactctgt actttgtcct tgaagcactg
     3301 aaacagtacc tgattcatct ggaaaaggct gttatcgtgt tgttgttctt cattgcggta
     3361 aaactgggcc tgaacgcgac cgatcacatc tggcatcatg gttacagcat cgctgcgaca
     3421 accagcctgt atgttgtact gggggttctg gtgctgggca ttctcgcaag cgttattttc
     3481 cccggcaaac ctgaatctga ggaaaagggg aactgatccc cttaagtgtt aaccggagca
     3541 attactaatc gaagaggtta ttttatgagt gtttctcttt ccaaaggcgg gaacgtctcc
     3601 ctgagtaaag cagctccgtc aatgaaaaac gtcctggtgg gtcttggctg ggatgcacgt
     3661 tcaactgacg gtcaggactt tgacttggat gcttcagcat tcctgctgac cgcaaacggc
     3721 aaagtgcgtg gcgattcaga tttcatcttc tataacaacc tgacgtcatc cgacggttct
     3781 gtaacgcaca ccggcgataa ccgtactggt gagggggatg gtgatgatga atcgctgaaa
     3841 attaaacttg atactgtccc gtctgacatc gacaggatca tcttcgttgt gacgatccat
     3901 gatgctcagg ctcgccgtca gaactttggt caggtttccg gggcgtttat tcgcctggtt
     3961 aatgacgata accagactga agttgctcgc tacgatctga ctgaagatgc gtccactgaa
     4021 accgccatgc tgttcggtga actgtatcgt cacaatggtg agtggaaatt ccgtgctgta
     4081 ggtcagggtt atgcgggggg gctggcatct gtatgtgctc agtacggcat taacgcgtct
     4141 tgatcgaaaa ctaacttttc attccgggct gttgattcag ccctcattca actgtttgca
     4201 ggagttaaaa atggcagttt ctctcgtaaa aggcggcaac atatctctga ccaaagaagc
     4261 accgaccatg aatgttgcta tggttggtct tggctgggat gcccgtgtaa ctgacggtca
     4321 ggcttttgat ctggacgctt ccgtattcgc agtaggtgaa gacggtaaag tgctgtcaga
     4381 tgcccatttc attttcttca ataataaaac cagccctgat ggcgcagtag agcaccaggg
     4441 tgacaaccgt accggtgaag gcgatggtga cgatgagcaa attaaaatcg atctggcaaa
     4501 agtctcagtt gacgtcaaaa aactggtatt tgccgttacc atctatgatg cagaagcgcg
     4561 taaacaaaat ttcggcatgg tgagcaacag cttcatgcgc gtttacaaca acgataacgg
     4621 cgcggaaatt gcccgtttcg atctgtctga agacgcctca actgaaaccg caatggtctt
     4681 cggtgaactg tatcgccatg gcgctgagtg gaagtttaaa gctgtcggtc agggtttttc
     4741 cggtggcctg tcagcgcttg cttcccagca cggcgtcaac gtctaaaaga tgcttagtct
     4801 gaccccggca atgccgtaat gccgatcggt taaggatcag ttgaccgatc cagtggttgt
     4861 gtaagaatcc ggaaacgctc actcgtttcc ggatttttta tgcacactgg acaggccctt
     4921 gatctggtat cccgttacga ttctctgcgt aacccactgg cttctctggg ggattacctc
     4981 gaccccgaac tcatctctcg ttgccttgcc gaatcaggta cggtaacgct acgcaagcgc
     5041 cgtcttcccc tcgaaatgat ggtctggtgt attgttggca tggcgcttga gcgtaaagag
     5101 ccccttcacc agattgtgaa tcgcctggac atcatgctgc cggcaatcgc cccttcgttg
     5161 cccccagtgc cgttattcag gcccgccagc acctgggaag tgaggctgtc cgccgcgtgt
     5221 tcacgaaaac agcgcagctc tggcataacg ccacgccgca tccgcactga tgcggcctga
     5281 ccctgctggc catcgatggt atgttctggc gcacaccgga tacaccagag aacgatgcag
     5341 ccttcccccg ccagatacat gccggcaacc cggcgctcta cccgcaggtc aaaatagtct
     5401 gccagatgga actgaccagc catctgctta cggctgagcc ttcggcacga tgaagaacag
     5461 cgaaaatgag cttgctgagc aacttataga acaaaccggc gataacaccc tgacgttaat
     5521 ggataaaggt tattactcac tgggactgtt aaatgcctgg agcctggtgg gagaacaccg
     5581 ccactggatg atccctctca gaaagggagc gcaatatgaa gagatcagaa aactgggtaa
     5641 aggcgatcat ctggtgaagc tgaaaaccag cccgcaggca cgaaaaaagt ggccgggact
     5701 gggaaatgag gtgacagccc gcctgctgac cgtgacgcgc aaaggagaag tctgccatct
     5761 gctgacgtcg atgacggacg ccatgcgctt ccccggggga gaaatggcgg atctgtacag
     5821 tcatcgccgg gaaattgaac tgggatacag ggagataaaa cagacgatgc aactgagcag
     5881 gctgacgctg taaaaagccg gagcttgtgg agcaagagct gtggggtgtc ttactggctt
     5941 ataatctggt aagatgtcag atgattaaaa tggcggaaca tctgaaaggt tactggccga
     6001 atcaactgag tttctcagaa tcatgcggaa tggtgatgac attgcagggc gcttcaccgg
     6061 gacgtatacc ggggctgatg cgcgatcttg caagtatggg acaacttgtg aaattaccga
     6121 caagaaggga aaggggcttc ccgagagtgg taaaggagag gccttggaaa gagcctgtac
     6181 atagatttgt gtaattgcct gattttgata tgttcaatca agcatcaaat gaaggttaat
     6241 ttatggacga aaaacagtta caggctctgg ctaacgaact ggccaaaaac ctcaaaaccc
     6301 ctgaagacct cagccagttt gaccgtctac tgaaaaagat cagcgttgaa gccgctctca
     6361 atgcagagat gacacaccat cttgggtatg agaaaaatca gtccaaaccg ggaactaact
     6421 cccgcaacgg ttattccaca aagaccgtta tcacaggcga cggtccactg gaactgcgta
     6481 ctccgcgcga tcgtgacggt accttcgaac cacaactggt aaagaaaaat cagacccgta
     6541 ttactgggat ggataaccag atcctctcgt tgtatgccaa agggatgacc acccgtgaga
     6601 tagctactgc gttcaaagaa ctgtatgacg cagatgtttc accggcactg atatcaaagg
     6661 tcaccgatgc cgtgatggag caggttgtag aatggcaaaa cagaccactg gatgctgttt
     6721 accccattgt ttatcttgac tgtatcgtcc tgaaagttcg gcaggacagt cgcgtcatca
     6781 acaaatcggt gttcctggca ctgggcatca atatcgaagg tcagaaagaa ctgctgggta
     6841 tgtggctggc cgaaaatgaa ggggcgaagt tctggctcaa tgtgctgact gaactgaaaa
     6901 accgcggtct gaacgatatc ctcatcgcct gtgtggatgg cctgaaaggc ttcccggatg
     6961 ccatcaacac agtatatccg aaggcccgca tccagttatg catcgtgcat atggtgcgca
     7021 acagcctgcg cttcgtgtca tggaaggact acaaagccgt cactcgcgac ctgaaagcga
     7081 tttatcaggc tcccacggaa gaggcaggtc agcaggcact ggaagcgttc gctgcggcct
     7141 gggacagtcg ctatcctcag ataagccgaa gctggcaggc taactggccg aatcttgcca
     7201 cgttcttcgc ttatccaacg gacatccgca aagtgatcta tacgacgaat gccatcgagt
     7261 cgctaaacag cgtgatccgc catgcgatca aaaagcgtaa agtgttcctg acagacgact
     7321 cggtgaaaaa agtggtgtgg ctggcaatcc agtctgcgtc ccagaaatgg acgatgccgt
     7381 tgaaggactg gcgaatggca atgagccgct ttattatcga gttcggtgac cgcctggacg
     7441 gtcacttctg agaaaaggca tttacacaga atcctaaaca ggctcgtcac ttctgagaaa
     7501 aggcatttac acagattcct aaacaggctc cctggaaata ccccacagcc ccgaaaaaga
     7561 gccagtcagt tgctgaactg actggcatta gctcccgggg cactttattg gtaagcgtca
     7621 acggagcacc gtattgacgc ttatttattg gtgagtacta cgttccatgg caggagttca
     7681 tcaactcggt tggaaggcca ttccggcagt acgctcagga tatggcgcag atacgcttcc
     7741 ggatcgatac cgttcagacg gcaggtgccg atcagcccgt acaacagtgc tccacgctcg
     7801 ccgccgtgat cgctgccaaa gaacata
//
LOCUS       DAZFFI010000173         7807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFFI010000173 DAZFFI010000000
VERSION     DAZFFI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.175"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..308
                     /locus_tag="ACHJ12_004617"
     CDS             <1..308
                     /locus_tag="ACHJ12_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992299.1"
                     /translation="VIARNYTFQNLTHLNKAYKEWLDIDVRAILYKKKRIGKSVDFLE
                     NRISEIIQYRHGIVHHFELDRTLNRDGYIHILDAIEKSIIEFIHYIEGKYKFKLNAY"
     gene            complement(429..1538)
                     /locus_tag="ACHJ12_004618"
     CDS             complement(429..1538)
                     /locus_tag="ACHJ12_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO4992300.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREASWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSHEGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            1695..2879
                     /locus_tag="ACHJ12_004619"
     CDS             1695..2879
                     /locus_tag="ACHJ12_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4992301.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVCIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            2879..3391
                     /locus_tag="ACHJ12_004620"
     CDS             2879..3391
                     /locus_tag="ACHJ12_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4992302.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3446..3811
                     /locus_tag="ACHJ12_004621"
     CDS             3446..3811
                     /locus_tag="ACHJ12_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992303.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            3820..3975
                     /locus_tag="ACHJ12_004622"
     CDS             3820..3975
                     /locus_tag="ACHJ12_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4992304.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3962..6769
                     /locus_tag="ACHJ12_004623"
     CDS             3962..6769
                     /locus_tag="ACHJ12_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4992305.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGLFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATIGSLSLVIGGLYGKLATLRLGFDILTRS
                     MNVIRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLIPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKRIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPATSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6782..7270
                     /locus_tag="ACHJ12_004624"
     CDS             6782..7270
                     /locus_tag="ACHJ12_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992306.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LF"
     gene            complement(7367..>7807)
                     /locus_tag="ACHJ12_004625"
     CDS             complement(7367..>7807)
                     /locus_tag="ACHJ12_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4992307.1"
                     /translation="YHTIVFNMGKQINHNGLDNVSLSPFSDAGCSFALVRHADFVITV
                     DTAIVHLASALNIRQYCIYNNRMHEGKFENNIVWGPNSKLATQLTTSEHLRSEGGDDM
                     HKFDIMILINAIKQDLTNDIPNYHSDLGCKNRSRNPELESSNSL"
BASE COUNT         1863 a         1854 c         2182 g         1908 t
ORIGIN      
        1 gtgttattgc aagaaattac acttttcaaa acttaaccca cctaaacaaa gcatataaag
       61 agtggctgga tatagatgtg agagcgattc tttataagaa aaaaagaatt ggaaaatcag
      121 ttgattttct tgagaatcga atttctgaaa tcatacaata tagacatggg atagttcatc
      181 actttgagtt ggataggacc ttaaatcggg atggatatat tcatatatta gatgcgatag
      241 aaaaaagcat aattgaattt atccattaca tagaaggcaa atacaaattt aaattaaatg
      301 cgtattaagc aatcaacctt acatagggat gacaaagtcc gcttctgaca cggagcggac
      361 cgtcgtttat gtttaattct atactgtaaa cttgccagtt taagtttgag ctaatatgtc
      421 taagttgctc actctgtttc tgctatccat tccgggattt ttgcctcaag ctcaagctgt
      481 gtggtaaagc cgctgttatc aatggtgtgc tcggctttcg caataatcca gtcctgatta
      541 tcaatctcgc ttttaaatcc tgttaccgtg ccatgcattt cggggtagag ttctgcacgt
      601 ccacgcgcca gcgtgatgga aaatgatgcg gctccgcgtt gtagctgctg ccactttgcc
      661 gccgctgcgc gtcttgctgc ctgctcgttc tgataagtct tgcgtaacac aaacacattg
      721 ccttccgcgc cttccatata atcaccttca tggctgctgc ttttctcctt tttgggtttt
      781 ggcggtttgc ggcgtttcac gctgactttt ttctttttcc cgtaattaag atcaagccag
      841 taagcgcgta cccccgtata cgcctcgcgg tcagcaatgc ggaactgatg gcgatcgccg
      901 ctgctgcgtg tgatggcgaa cgatggcaac ggctggccct gtgcgttcac gccaccgccg
      961 ggcatgatga ataacagatt accgcttttt accgtggtga ttgcgcccag catttccgcc
     1021 atgcgcgtaa ggaaggacat gtcgctttct tcagtctggt cggcgtggtc gatttcgata
     1081 tccatcagca tttcgctgat ttgcggtttc aggccgtacc gatgagcgat ggcggatacc
     1141 acacgttcaa cggtcacatc atgccaggag gcctcacgtt taacattaaa ttcatcccga
     1201 aaatctgcgc ttctggctga aacagtcagc ctgtccggcg gtccttcgtg agcgatttca
     1261 tcaacaatgt aagtgccttt ttctgtcagc ggttcccctt tccagccaat gagaaccgtc
     1321 agacgcgcgc cccgtggcgg tagctgcaac tggccatcgg catcatccag cgtgatggtg
     1381 agctggtccg cctcaaatcc ccggttgtcg gtcagtgaca ggctcatcag gcgttctgcc
     1441 acgccggaca gcgttttacc ctcggcgaga atatcaaaat ccggcatttt cacggggtct
     1501 gtgccctgac tgagcaattg catggtggtg tcggtcatct ttccctccct gtgcggcatg
     1561 gtcgcatgtg cgtgcggagg gggttactgc tttttgttgt cgccgtggcg ggagaatagc
     1621 gcaggggtga gattacgcgc gtggtgggtg atgattgttg ctgaatcatt taacggatac
     1681 aaggggctga agctatgagt gaaactcgtt ttcatggtgc ccgtgttacg gaaaataccg
     1741 acctggtaac agcgattaac gatgttgatt ccagcgttat cggtatcgtg gcaacggcgg
     1801 atgatgcgga cgcgaagctg ttcccgctga acaagcccac attgctgacc cgcgtcaatg
     1861 acgtgctggg aaaatgcgga acaacgggga cgctttatcg tgcgcttaag gccatcgcag
     1921 accaggtgag cacaaaggtg atcgtcgttc gcgtggctga acacaaagaa gaagacggaa
     1981 agacgcagga tcaactggtt atcggtggtt ctgaggatga cggcagctat acggggatgt
     2041 atgcgctgct tgttgcagag caggatgaaa gcatcggata ccgtccgcgt attctggccg
     2101 cgccggagct ggacacggag gctgtaacaa aatccctgtg cgtgattgca ggtaaactgc
     2161 gcgcgtttgt gtatgcctca tgtcacggct gtaacacgat ggctgaggcg attacctatc
     2221 gccagaaatt caacgaacgt gaggtgatgc tcttatggcc ggacttcatc gcctacaacc
     2281 cgaaaagtgg caaaaacgaa acgttccccg cgcctgccta tgcgtgcggc cttcgtgcgt
     2341 acattgacca tgagcaggga tggcacaaat cactgtccaa cgttccggtt aaaaatgtgc
     2401 tggggatgtc gaggcatgtg ttctggtcgt tgcaggccga agacagcgat gccaacagcc
     2461 tcaacaacaa agaaatcacg accattattc gtcgcaacgg gttccgcttc tggggcaacc
     2521 gcacaccgga aacgaacgcc tacatctttg aggtgtatac ccgaaccgca caggtgctgg
     2581 ctgattcaat tgcggaagcg cagtttgaaa ctatcgacag tccactgacg cctgcgaacg
     2641 tgaaggatgt tatcagtgcc atcagggcaa aactggattc actggtgact gccgggaaac
     2701 tgattggcgc ggagtgctgg tatgacgtgg tggataacag caccacggat ttacgtcagg
     2761 ggcgtgtgtg tattcgctac aaatatacgc ccgttccgcc actggaagac atggagcttt
     2821 accagacgtt tactgatgaa tactttgaac ccgcatttgc ggtgctggga ggtgcctgat
     2881 ggctgtgcca aaacatcttc gcttttttac gctgtttgtg gatggtgaaa acgaagtggg
     2941 taaggtgacg tccgtcactc tgcccaaact gacgcgcaaa accgacagct accggggtgg
     3001 tggcatgatg ggggcggtaa gtattgatct cggtctggac gactccgcgc ttgatgcgag
     3061 ctttgtcatg gggggcgcag ttcgtgagct gttccttaag tatggcggca cgattgacgg
     3121 cacgctgctg cgttttgcgg gtgaatacta caccgatgca gaaagcgacc tgtatgaagt
     3181 cgaaatgcgc ggacgtgtga cggaaattga tatgggggaa gccaaacagg gcgaagccac
     3241 atcacacact tacgccatta aaaacaccta ctacaagctg agtgttaacg atcgcccgtt
     3301 gtgggagatt gacctgctga acttcattta ccggaaggac ggcaaggaca ttgtgcccga
     3361 tcgcatccgt tccgcgcttg ggcttggctg ataagtaata tgcaggcggc gcagtgcgtc
     3421 gcctctgact gaaaggagtt tcctgatgaa agagacgaaa aacatcgata ccgaaaacac
     3481 ggtagttact gacactgtga aagaaaccag tgagcgtggc gtaaaactta cccaaccaat
     3541 tgagcgaggc ggcgaaaaaa tcacgtatgt ggagatcacc ggggctattg agcaggctgg
     3601 atctctgcga gatttgtcgc tgtctgatgt gctgaatctg aaagcggaat ccatgtttac
     3661 gctgctgtca cgcgtgacat caccgcgact ggatgaagtg acgatcaaaa aaatggcatc
     3721 ccgtgacttt attcagttat gtgtggttgc cgtaaatttt ttgagcggtg cggactctgg
     3781 cgggaagaac gaacaggcga cggaagcctg atcacggttg tgtgctttga gcacatagaa
     3841 gactttgtgg cagatattgc cgttattttt aactggtcgc ccgccgaaat cttcatgatg
     3901 acgcccggcg aagtggttag ctggcgtgag cgggcggcac ttcgcagcgg gaatgcagac
     3961 aatgaagact cttgatatcc gggtcgcttt cagcgccgtt gacaggctga cccggcctgc
     4021 cgaaaacgcc cgccgcctga tggggctgtt tggtgactcc atccagcgaa cgcagggggc
     4081 gatcaaaaat ctcgagcgtc aggcgcgatc atttgagcgc gcccgcgacg ctgtcagtaa
     4141 agcggatgcg ggcatcgtga aagcacgacg ccagcttaac gcccttaatc agttacaacg
     4201 cacgggtaca gtgctcagcg aaaaacaaca aaagctgatg cagcagttaa gcacccggct
     4261 tgaacgcctg aatgaatcgc gcacacgtga aattcagaaa atgcgggaac ttggcggaga
     4321 gctgaaacgc cacggcattt ccctgacagg cagcgataac accatccagc aggccatcag
     4381 acgcaccgaa cagtacaaca accagcttga acgcgaacgg caggcgcttg cgcgtgtaac
     4441 gcgggcgcgt gagcggtatt cgcgcgcgca ggaaaccgct ggaaaactga aaacaggtgg
     4501 tgcgctggca attggtgcgg cagcggcggg cggctatgct gccgggcgtt ttttgcagcc
     4561 tgcgatcggg ttcgggaaag agatgtcccg cgttcaggca ctgacgcgaa tcgacaaaaa
     4621 cagcccgcag tttaaggcgc tgcgtgagca ggcgttaaaa cttggctctg aaacacagtt
     4681 tactgcgagt gatgccgcca gtgggcagag ctttctggca atggctggtt ttactccgca
     4741 ggccattcag gccgcattgc ccggtgttct taatatggcg ctggcaggtg gcgtcgaact
     4801 cggcgagacg gctgatatag gctccaatat cctcacacag ttcaacctga cagccgatca
     4861 aatggaccgt gttggcgata cgctgacagc agcattcacc cggaccaata ctgatttacg
     4921 cgcgctgggc gaaaccatga agtataccgg tccggttgcc gcaaaacttg gtatcagtct
     4981 tgaagaagcg gcagccatgg ccgggatgct tgccaataat ggtcttcgtg gaagcgatgc
     5041 tggtacagcc atgcgcgcaa gtctgtcccg ccttgcatca ccgccaaaag ctgcggctga
     5101 tgcgctgaaa gagctggggg tgtcagttgc tgacgccaga ggcaaaatgc gcccgatgga
     5161 ggatgtgctg cttgatctct acaaggcgac acaaaaatac ggacaggtgg accaggtttc
     5221 cttcttcaag gacatcgccg gagaagaggc gttcgttggt ttgcagacgc ttgttgcggc
     5281 ggctggttca ggagagctgc aaaaactgac cagagaattg cagggggcaa ggggagaggc
     5341 cgatcgcgtt gcaaaagtaa tggccgataa tcttgatggg gacctgaaaa atctcgacag
     5401 cgcatgggaa ggtcttcgta ttcgcatcag tgatctggtt gacggtccgc tgcgttctgt
     5461 cacgcagtgg ctcacgcggg tgcttgaaaa aatcacctcg ctggcgcagg cccatccggt
     5521 actgacgcgc cagctactga tagcaggcgg tgcgttgctg gcaatgactg caacgattgg
     5581 ctcgttgtcg ctggttattg gggggcttta cgggaagctg gccaccctgc gtcttggttt
     5641 cgacattctt acccggtcaa tgaatgtcat cagggtgttg cctgcgctgt ggggaatggt
     5701 gacgggttct gtttctttgc tgggaggcgc tatcggggcg ctgttcagtc cggttggtct
     5761 tatcgtagct gcgcttgccg gagctgccgt tcttatctgg aaatactggg atcccatcag
     5821 ggcatttttt gccggggtgt tcagcgggat tatggaaagg ctgatcccgt tgcgcgaaac
     5881 ctttgaacgg tttggtcctg tttttgacgc aatcggaagc gggatcagcc aggtgtttaa
     5941 ctggtttaaa tcgctgctgt caccgatgga gtccagcaag gaaactctgg ataaatgtac
     6001 cagtgctggc gagatattcg gtaacgttct tggtggcgcg ttacagcttg ttctgacgcc
     6061 tgcaaaaatg ttgctggata cgctggcgtg gatacttgaa aaactcggtg ttcttccgga
     6121 tgaagctgaa agggcgagaa agagaatcga agacgcacag cgtgcggcca ttcttcagga
     6181 caaggttgcc ttgcttcagg gggacctggc gaaaatcaat ccgccgaagc ctgtggaaaa
     6241 tggcaatggc accggaggtg ataaacccaa agacaataaa ccgctcacag acagcaatac
     6301 cgggacgcta cgcagactca gcaaaattgc tgataataca ggtaagctgg ttgatgagac
     6361 gaaaaaacgc attggccccg gcgatattgt ctttaagaac ctgccccgcg cacttgctgt
     6421 tcgtggggag tggcaggagc ggaagattgc gcaggtcagt aagcctgccc ccgcaattaa
     6481 tatcacacct gtggttccgg ctccgctgcc tccggcgctg gtccctgttg ttgcggccag
     6541 ctcccgcccg gtggcagagg ccatacgatc gccagtggca tcagttcctg caacttcccg
     6601 taaccgggag cctgttgcct ccggatttgg cggtgaaatt catgttcatc tgcataacgt
     6661 tgttacgcag aatccccgcg aactggcgaa actggtcggt gaaatggtca gggcagaaat
     6721 ggaacggcgc gcccgtgccg ggcgtggaag tttttacgat aaagattgag gagtcatggc
     6781 catgatgatg atctacggca tgtttgtttt tgagctgcgc acgctgccgc atcagcagtt
     6841 acagcaaaac aaaagctggc ggcatgtgaa aaatgaacgc gttaaccgtt cagcaagctg
     6901 gcagtatatc ggtgcaggtg atgatcgcat cgtgctttct ggtgtgcttt atcctgaaat
     6961 tacaggtggc gaagtgtcgc tgtcgctgct gaccacgcag gcgtatacag gacgaccctg
     7021 gcctttgatt gatggtgtcg ggcagattta cggcatgtat gtcctgaccg gaacgaatac
     7081 gacccgttcc gagtttgatc gctacggtaa ggcgaaaaag atagaatttt cactgaccct
     7141 tgaacgctgt gatgaggatt tgcgggagcg cctgcaatcc tcatcgttca gcgatatgct
     7201 gtccggcttc aaagataagg tgacatcatc tcttaacagc gcgaccagtt cagttaaagg
     7261 actgttctga ttttacattg ccgccaatac cctcatattg ggtaatgggc ggcttgttgt
     7321 tatataatat atcgtaggtc ctgataaaac tgttgaggtt tctactttat aaagaattgc
     7381 tactttctaa ttctgggttt ctggatctat ttttacaacc taaatccgag tggtaattcg
     7441 gtatatcgtt tgtcaaatct tgtttgatag cattaattaa gatcattatg tcgaatttat
     7501 gcatatcgtc gcccccttca gaccttagat gttcggatgt ggtgagttga gtcgctaatt
     7561 tgctgtttgg cccccacaca atattatttt cgaattttcc ttcatgcatt ctgttattat
     7621 atatacagta ttgtctaatg tttaatgcac tggctaaatg tacaatggca gtatctacag
     7681 ttataacaaa atcagcatgg cgaaccaatg caaatgaaca tccggcatca gaaaatggag
     7741 atagagacac attatccaat ccattgtgat taatctgttt tcccatatta aaaactattg
     7801 tgtgata
//
LOCUS       DAZFFI010000174         7759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFFI010000174 DAZFFI010000000
VERSION     DAZFFI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.176"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..1105
                     /locus_tag="ACHJ12_004626"
     CDS             47..1105
                     /locus_tag="ACHJ12_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4992308.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKLFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNNQWFEEHRTAIESTGATLT
                     TGATGIDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTRLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSALPMAVFNHPEHRLVSPQLYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNQGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNLLN"
     gene            1115..2257
                     /locus_tag="ACHJ12_004627"
     CDS             1115..2257
                     /locus_tag="ACHJ12_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4992309.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPILFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSENALSRRSTLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGKWDIQGKQSWGRLGMLAPAADLGHEVSFRKGER
                     VLVLGTGEFVWEPFLLAERLEAAGAQAFYGATTRSPIAVGYAIKSAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTRLLKALAEVAPVVEIVTYE"
     gene            2250..3023
                     /locus_tag="ACHJ12_004628"
     CDS             2250..3023
                     /locus_tag="ACHJ12_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992310.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLTPYQEKLASMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFSTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3025..4104
                     /locus_tag="ACHJ12_004629"
     CDS             3025..4104
                     /locus_tag="ACHJ12_004629"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4992311.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVDQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGTALDIIEERHGTSGIVFVDGWTGKG
                     AITGELVRALRDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLLSR
                     SVWSSEGLHGCMVCEHLSEFECSRMLVDTVAHFRKKLTPSSLAPLSWNTESTRVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIIVTEMGGTLGQYRAVTIIKKVF"
     gene            4104..5060
                     /locus_tag="ACHJ12_004630"
     CDS             4104..5060
                     /locus_tag="ACHJ12_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4992312.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHEKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMRSLGKNNWPLLFIRPRHAEMGKWLKEHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMGGTHLSMMPTLETENVFDVIQMRELATRLLEHPCHKRI
                     IALRIGGNDLMNVVSLRRPRALTLYDSPMGYVIKMLVSVFSPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPDQIEVIQNALMVTQGEYSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLHNEQSADGIRLLTITQHH"
     gene            5071..6166
                     /locus_tag="ACHJ12_004631"
                     /pseudo
     CDS             5071..6166
                     /locus_tag="ACHJ12_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6184..6651
                     /gene="terW"
                     /locus_tag="ACHJ12_004632"
     CDS             6184..6651
                     /gene="terW"
                     /locus_tag="ACHJ12_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4992313.1"
                     /translation="MQLNTRQARIFKLANLLGSGKPVSATDIITYLDCSEPTLTRALK
                     ELRKSYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNADLKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7020..7673
                     /locus_tag="ACHJ12_004633"
                     /pseudo
     CDS             7020..7673
                     /locus_tag="ACHJ12_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         1966 a         1928 c         1977 g         1888 t
ORIGIN      
        1 tcacttctcg acaataaatt ttcttgccgt tccggataat aaaaaaatga acaaaaaaat
       61 ttggtttatg gaaggtttgt cctctcagag agatattatt cagggggtta aattatttgc
      121 acaaaaaaat aattttgcca ttaccgtttt tgcctcccac cgtaacgaaa gaaatgaaat
      181 cctttccgtt gccgattatt ctttgactga acctgaagat cctcaaaaac gtcttcagtt
      241 tattcaggaa accattcaga cctacggcat ccaccatatt catactggtc gtaacaacca
      301 gtggtttgaa gaacaccgaa ccgccattga atcgaccggt gccaccctta ctaccggtgc
      361 aacgggcatc gactggttaa ctctggctga cgaaaaagtt acttttgctc agtttatgga
      421 gcaaaagggg ctcccggtcg taccatcctg gcgggtgaat acgctggcag aattaaagac
      481 acgcctcgcg gctccgccgt tcactgacag tccggtatgc gtgaagccgg tgacgggtat
      541 ctacggcatg ggattctggc gctttgatga cagtgctttg cctatggccg tctttaatca
      601 ccccgaacat cgtctggtca gcccgcaact gtatattgct gcagcgtcgg cttctgagtc
      661 gtttaaaccc cttgttttga tgccgtacct gcctggcccg gaattttccg tcgatatcct
      721 cgcggataag ggcgaaatac tcgcagccgt gggacgccgt aaggaagggg ctatccagta
      781 tctggtaaac caaggaagcg cctgggaact ggcgtgtgac tgcgcccggg ttatgaaggc
      841 cgacgggctg gtgaatgttc agacgcgaaa cgatgtgaat ggcaacccgg tgctgcttga
      901 aaccaacatg cgtccgtcag gcggggtggg ttataccctt cacagcgggg ttaaccttcc
      961 cgggttgttt gctgctttta agctcggtct gatgtctgaa gatgcggtga tccagagcgc
     1021 cagaaatacc ttttctccgg ttgcagtgag atccatcaca gatgtcatcg tttacccgga
     1081 atcactctct aaccttctga attaaggcgt gtttatgtct gataccctcg aagatatgat
     1141 ttatcgccgt accctctcat gcggtacgat tcaggtaacc cgcgaccagg gtgaagtttc
     1201 gctcgatgac ctgtttgata tcgctgaaag acgtaacccg aaacgcgcct ttctgtttgt
     1261 cagcaaagta ctcggcaggc acattcccgt gccaccctca gtcatgcggc aggcctacag
     1321 acagcttgcc agccagttcc cctcaacgct gacagggccc atactgttta tcggtatggc
     1381 agaaaccgcc gttgggcttg gggctggtgt gtttgatgaa gtgcgccacc agcatcgtga
     1441 atctgtctat ctgacctcta cccgtcaccc ggttgatggc acattgcttt gcgagttcaa
     1501 ggaagaacac agccacgcca ccgatcatct gatctatctg ccagacgatg aagagaaaag
     1561 acgtcgtgtt acaaacgcgc gaactctggt tttgattgat gacgaagcaa ccaccggtaa
     1621 tacctttatt aacctgcttt ctgccctgcg taataccggc aagcttcaac atattgaaca
     1681 ggttatcgct gttacgctta cggactggag cgaaaatgcc ctgtccaggc gcagcacttt
     1741 acccgtcact tcggtttctc ttgtaagtgg tcagtgggga tggactccat taccagatgc
     1801 acctgtcccg gacatgccga aggtcaacgt aacttcacgt ggaaaatggg acatccaggg
     1861 aaaacagtcc tggggtcgac tggggatgct cgcacctgcg gccgatctcg gtcatgaggt
     1921 atccttccgc aagggggagc gtgttctggt tctcgggaca ggggaattcg tctgggagcc
     1981 gttcctgctt gctgaacggc tcgaagctgc cggagcacag gcattttatg gtgcgaccac
     2041 ccgctcccct atcgccgttg gttatgccat taagtccgca atttccttta cggataacta
     2101 cggcctgggc atccccaatt ttgtctataa cgtcgcccat cagcaatttg accgcattct
     2161 tgtgtgtact gagacacctg cagaaagtat tgacacgcgg cttcttaaag cgctggctga
     2221 ggttgcaccc gtcgtggaga ttgttaccta tgaataaacc cgttgttctg agcgaccttg
     2281 acgacacgct ctttcagacc cgtcgtaaaa tggtagacga gctggccctg gagccattcc
     2341 gtaccggtgc cgttgatcgt actctgaatc cacgaagctt tatgacagaa gaacagtcca
     2401 tgttagtgga ctggctccta gagcaggctg aactcattcc cgttaccgca cggggaactg
     2461 aagaaatcag ccgcgtaagg atccctttcc actcctgggc aatcaccacg cacggggccg
     2521 tcattctcac gcctgaaggc aaacccgacg aggagtggaa ggcccatatg ctcggccagc
     2581 taactcccta ccaggaaaag ctggcatcga tgcagcgtct gatcactgaa atgatggacg
     2641 caaaagggat caatgcctgg gcaagactga acttcgaata cggtgaaacg gcggtttata
     2701 tggtgatgaa acaccgcgac agcactcgcc ttgacgaact caatgccatt gcagatgaga
     2761 tagaaacggt attttcgacc gagggcttct acatccaccg caacagtaac aacgtagcct
     2821 ggcttcccac cccggttgag aaagggctgg ccgtcagctg gctgcttgaa aaacttcggg
     2881 ctgaacgcgg agttttcccc gtaattggtc tgggcgacag cctgagcgat catcgtttta
     2941 tgaaactgtg cagctggttt ggcatcccgc gtcagagtca gtttgcagat gccatttcac
     3001 agcgaatttt tggagaaaat taaaatgcag ccacatgaaa catttaccgg ctcataccag
     3061 cccggtgacg tggaatttct gctaaagccg gttgtcattg aaatgacgcc agttgatcaa
     3121 aaagaagagc tgattcagtc cgggaagaaa cattattcgg acatgctcag ccaggagcca
     3181 gcgccgacac aatggcatct tgatttgttt caccgggctc tggatcgggg cgcagagaga
     3241 cttgcaaaag aagtcacaca gctcgctatt gctctggccg aacgcttcgg tgatgagccc
     3301 atagtactgg ccagtcttgt cagggctggc gtaccgctcg gcgttatgct acaccaggct
     3361 ctgcgtgaca tgggaaaaac ctcatggcat tacggcatca gcatcatccg ggatcgtgga
     3421 attgacggta cagcacttga tatcattgaa gagcggcatg gtaccagcgg cattgtcttt
     3481 gttgacggat ggacaggtaa aggcgcaatt accggagagc ttgtacgcgc actgagagat
     3541 cgcccgggct atcctgagca gccgcgactg gttgtcctgg ccgatccctg tggctgttcc
     3601 tggcttgcag caagtgatga tgactggctc attccttttg gcatcatggg ggctccggtg
     3661 tcaggcctgc tctcccggtc agtatggtcc tctgaaggat tacatgggtg catggtttgc
     3721 gagcatctca gtgaattcga atgcagccgg atgcttgtcg ataccgtcgc tcatttccgt
     3781 aagaagttaa caccgtcatc cctcgctcct ctgagctgga atacggagtc aacccgggtc
     3841 ttatggcaga caagtcgcga cgttatcgcg ttcctggccg atgaatttaa agtggacagc
     3901 gtcaaccgta ttaaacccgg tattgctgaa gcaacccggg ctgtattacg tcgggtaccg
     3961 gaccatgtat ttgtgcgttc gattgacgac ccggacgttg ccttacttgt ggggcttgct
     4021 cgtgaaaagg gaataattgt tacagaaatg gggggaaccc tcggccagta tcgggctgta
     4081 accattatca aaaaggtatt ctaatgaaaa atcgtctttc tccctggaat cttggtgcca
     4141 ctctatacat gcctgcaaca cgggaagata ttgctgatgc cgttctgcac gagaaaatcc
     4201 cgggtttgcg ctctctggtg atttgcctgg aggatgctgt cagtgaagca gacatccccg
     4261 tcgccctcaa aaatctagag cacctgctgc acgaactcag taacagcatg cgtagcctgg
     4321 gtaaaaataa ctggcccctg ttgttcattc gcccccggca tgccgaaatg ggcaaatggt
     4381 taaaagaaca ttatgatctt tccgctgtcg acgggtttgt gttaccgaaa tttaccctaa
     4441 gttcgttagc tgaatggtgg gatatcatgg gcggaactca cctgagcatg atgccaacgc
     4501 tggaaacaga aaacgtcttt gatgtgattc agatgcgcga gctggccacc cggctgttgg
     4561 aacatccctg ccacaagcgc attattgcgc ttcgaatcgg cggcaacgat cttatgaacg
     4621 tcgtgtcgct tcgccgtccc cgagccctga cgctgtatga cagtccgatg ggctacgtca
     4681 tcaaaatgct cgtttcagtc ttcagcccgc gtgattttgc cctgaccgcg cccgtatgtg
     4741 agcatattga cgatcatgcc gttatggcca gagagctggc tcttgatatg gcacatggac
     4801 ttgttggaaa aacggccatt catccagatc agatagaggt cattcaaaac gcgctgatgg
     4861 tcactcaggg tgagtactct gacgctctgc ggatcctgaa ctctacccag gccgtgttta
     4921 agtcgcaggg ggcgatgtgt gaacctgcca cacatcgccg ctgggcggct ggcattctgg
     4981 acagagcccg gttttacggg ttacataacg agcaaagcgc tgatggaatc agattactta
     5041 ctataaccca gcatcattaa ggaaatcata atggggttca ggttcagagg tacttccgtt
     5101 tctttctgta gccggggggc ctatgccaat ctggggttac ccggtaccga gctgagttac
     5161 cgtacccggt ttgagctggc cgcgccttcc ggaggtggaa acaggacagc aaccgtcccg
     5221 gatctcagac agtcgcttga gcaggaagca gttgaactca tgtcagcggt gaccgcaatt
     5281 cgtaacattc acgagttgac gctggatcca aaaacaggca tcaattgggc agagctggaa
     5341 gcagtatacc tgcatagcag aacatcgctt tttcaggttc cggcaccagt gcgtcctgaa
     5401 aaaccagaat accatgcatt gtcggaaaat cctgcaggga acaaaggcat tgtttttctg
     5461 ggtaaatggt ttgaatcgga atcagctaaa gctgagcgtc aagcagggaa tcttcgccga
     5521 tggcggcaag aactgattga tgtgaagcgt gagaatcccc ttcgacagca ccggtaccag
     5581 caacggcaga cgaccagggc cgaacagtat tcaaactgga agtgtgatgc tgaagaacat
     5641 gaaaaaaggc tcgccacgac tcaggcagat gcccggcagt tgttccgggc agacgcagcg
     5701 tttttcgaat cataccttgc gggtgtgctt tcagaaactg aatggccgcg tgaaacgctt
     5761 gttgcatttg aagtaaagcc ggagatatca acagtcctgc cggatgttgg tttagctgag
     5821 attgaagatt tccctgataa gatttacggc gtcaatgccc ggggcatgga gctgacgcaa
     5881 aaagccatac gcgaaaacta tgcccgccac agccatggct gcttgttccg actggtcggt
     5941 atcgtgttac atacgctacc tttcgacaag gtgatcgcat caggatttac acaacgagta
     6001 agcggaacga ctatctggag gatgtttata tcctgtcctg caaatgcact cgcagccaga
     6061 aggcatagaa catattgatc cggttgaagc gttaggctat caccctgtta ttcgaaagat
     6121 gagaaacaca ttcatttttc agcctattga accactaacc ctttaagcaa actaaatatt
     6181 acgatgcaat taaataccag acaggccaga atttttaagc ttgccaactt actgggctcc
     6241 ggcaagcctg tttccgctac cgacattata acctatctgg actgctcaga gccgacatta
     6301 acacgggccc tcaaagagct acgaaagtca tattctgcag aaatcaaata cagtaaggca
     6361 ggtcattcct atcatctcgt taatccgggt caactggata aaaagacact tcgccggatg
     6421 aatgaggcgc ttgcacagaa tgctgatctg aaaaccggtg agtctacagg aaaggtcgta
     6481 ctcgataagg ataaaaaaac agccgtatca ttatcgctac gcatgcgaat tttaagaaaa
     6541 attgaccgtc tggctgcact gagtggatcg acccgaagtg aagctgttga aaaactggca
     6601 ctgcattccg ttgacgaact gataaaagaa tacagcgcta aaaaatcctg atgacaaatc
     6661 tcagctcggg gtgaataccc cggttttttc atatttcaac tctgtcttgg cgtgacgtat
     6721 ttttgatcta ttctcctccc gcaatgccag cagctcctta cagcctacaa aacctagcgt
     6781 cttgacatcc tcttgtgcta cgcatcaatc tttgggcgat ttacactcca ctcatgcaaa
     6841 tctgtgatgt atgatgattt tcagtaagtg taaactgact gccgtatgta gccattaagc
     6901 cagtattggt aacgtagatg cccacctttt cacttatttg gtggacacca ctttgtctaa
     6961 ctcgtcagat tctgaccaaa cgtttcaggc tgtacgctta ctctgaaggg gggagaatag
     7021 tagcccgtga tagcattgag ctaactgaca gccagatcgc agcactaaag cgtaaagcca
     7081 gtgatgacga ggcctgtggc gaaagcgaaa cggctcatcc gggatatctg ggttcgcagg
     7141 acacgttcta tgtaggcaac ctgaaaggtg tcgggcgtat ttatcagcag acgttcgttg
     7201 atacgtactc gaaagtggct cactgtaagc tttacgtcac caaaacaccg attaccgcgg
     7261 ctgacttact gaatgatcgt gtactgccgt tttatacgtc tcagggcctg ccgatgctga
     7321 gaatactgac cgacagaggc acggagtact gtggcaaagt agagcagcat gattatcagc
     7381 tttatctggc aataaacgat atagaacaca cgaaaacaaa ggcgatgtcc ccgcagacca
     7441 acggcatctg cgagcggttc cataaaacaa tcctgaacga attttatcag gtaacgttcc
     7501 gtaaaaaact gtacggcgat cctgatacat tacagtcgga tcttgatgaa tggctggctc
     7561 actataataa tgagcgaagc catcagagaa aaatgtgcaa tgggcggacg ccaatggaaa
     7621 cgttacttga tggaaagcgc atctgggccg agaaaaattt aagccagatg taatctgaca
     7681 gatacctgta taaataaccg gtaactgtca gatcaggtct gagctaatac aaattaaggt
     7741 tttaaattca ttaagtaat
//
LOCUS       DAZFFI010000175         7750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFFI010000175 DAZFFI010000000
VERSION     DAZFFI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.177"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..790
                     /gene="nanC"
                     /locus_tag="ACHJ12_004634"
     CDS             74..790
                     /gene="nanC"
                     /locus_tag="ACHJ12_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4992314.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            810..1916
                     /gene="nanM"
                     /locus_tag="ACHJ12_004635"
     CDS             810..1916
                     /gene="nanM"
                     /locus_tag="ACHJ12_004635"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4992315.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1981..2961
                     /gene="nanS"
                     /locus_tag="ACHJ12_004636"
     CDS             1981..2961
                     /gene="nanS"
                     /locus_tag="ACHJ12_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4992316.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDKFVEAILQFWREK"
     gene            complement(2969..3073)
                     /locus_tag="ACHJ12_004637"
                     /pseudo
     CDS             complement(2969..3073)
                     /locus_tag="ACHJ12_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3173..4045
                     /locus_tag="ACHJ12_004638"
     CDS             3173..4045
                     /locus_tag="ACHJ12_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO4992317.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELTEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRRNYPY
                     EGFVEWHRKEVFRG"
     gene            complement(4108..5418)
                     /locus_tag="ACHJ12_004639"
     CDS             complement(4108..5418)
                     /locus_tag="ACHJ12_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFO4992318.1"
                     /translation="MKSSSHFTVFEYGHLVSEEGDARINNAVVLPADLFKWLELRCLK
                     DDADNTHRLLTLSSRNGVKTLQVKNYAGVISLPGGRFLEILPKTGQDNEDTVRQQLLM
                     MLRTLKSFRHIASSESGVKISKMPLMDIFIQQFIESVRKIVQRGLKRDYLRQEDNLPW
                     IKGRLRISAQLSKNCIRRDLFQVEYDEYSVNRLENRILKTAINKISRQTSNPQLLQQI
                     TQLQFHFENITSVHDAYIAFEQLHFDRHMHHYEQALAWAKMILLGDSPHCMYGDVNAF
                     SLLFPMEAVFESFVTTWMRYRYYDKWRVDAQVSSKNLISYNGKALFKLRPDICLRPRK
                     STTGSVITCDIKWKIVNGRKDSLEQSQADLYQMLAYGLNYQEGEGDMILIYPYHNGFN
                     QPSPHPYEFSHQKENRLRLWVVPFFIGESLQTSELRFPSGAEFI"
     gene            complement(5415..7469)
                     /locus_tag="ACHJ12_004640"
     CDS             complement(5415..7469)
                     /locus_tag="ACHJ12_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HFO4992319.1"
                     /translation="MVQDSSALAPFFRAQIEKNNQYNSVWGKHYRQLVEQIQAEKPNF
                     SEATIRQVWYERDNGVSSLMQGGMSLVEFENAQKDLLTLTRQIAEECTQENYNRVIER
                     LIKLKADGVLNKVYRALCNRAFAAIYPSKISSVVNVNNFFSCYNYCNNHFQLGLSGER
                     EWLARNVDLKETLNRALGDDIDPIELNMSLWHLYAYEIQDKNAIGLVGSESALQENEQ
                     EDDVISSPLPKNTILYGPPGTGKTYRTIEIAVQICDPIAYSLQEGKDESEKRRELKKI
                     YDQLIKDNRVRFITFHQSFGYEEFIEGLRAETTDDGNVKYEIKAGIFKQICEDAAFGH
                     AGAQEMLDSALTRLQERLSEGENITLETQQGKAFQLTYKSQTNFGIFPSQSKKEDLGQ
                     GYNAYLKDIRQVYQDPRAKVHNPSYVRSILNYLINQRYLPLNTQSVASEKKENYVLII
                     DEINRGNISKIFGELITLIEPSKRAGEPESLSVLLPYSSTPFSVPNNLYLIGTMNTAD
                     HSLTALDTALRRRFDFEAMLPDITVLKDTVIKGIDLPRLLQTLNDRIQVLYDREHMLG
                     HAFFIPVVQAKEDEALAFERFKRIMRNKILPLLEEYFYNDWQKIRMVLADNQKEEAPD
                     LQFVREVKYQKKYADLFGDNGTDDLGTSFHLAAEDDNVWDNPIAWQQIYAPQKEKRRS
                     AE"
BASE COUNT         2247 a         1667 c         1660 g         2176 t
ORIGIN      
        1 ctacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa
       61 atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc
      121 gtcaccatta atttctcagg ctgcaacact ggacgtacgt ggtggatatc gtagtggaag
      181 ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag
      241 catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa
      301 tgatgttcag gttgaagtta attacgcgat taaacttgat gatcaatgga cggtgcgccc
      361 gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc
      421 ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta
      481 ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata
      541 tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa
      601 acagaacgat tatcgctatg caaaccataa gaaatgggcg acggaaaatg catttgttct
      661 acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc tggaccgtca
      721 gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt
      781 taaactgtag tagacaggag acagtcacaa tgaataaaac aataacggcg cttgctatca
      841 tgatggcttc atttgccgca aacgcgtctg tattacctga aactcctgtg ccatttaaaa
      901 gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacgg
      961 catggtacaa gctggataca caggccaaag ataaaaaatg gacagcgtta gctgcattcc
     1021 ctggtggacc aagagatcaa gcaacctcgg catttattga tggcaatctg tatgtgtttg
     1081 gcggcattgg caaaaatagc aagggcttga ctcaggtatt taatgacgta cacaaataca
     1141 accccaaaac caatagttgg gttaaattga tgtcgcatgc accgatgggc atggcgggtc
     1201 atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct
     1261 tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcagcaact atagacaaga
     1321 tcaatgccca ttattttgac aaaaaagcag aagattattt cttcaataag tttctgttgt
     1381 cttttgatcc ctcaacacag caatggagtt acgctggcga atctccctgg tacggaacgg
     1441 ctggtgcggc gcttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac
     1501 caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga
     1561 ataagcttgc tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca
     1621 atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa aattaccaga
     1681 acggtaagaa ctatgcgcat gaaggcctaa aaaaatcata tagcactgat attcatcttt
     1741 ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat
     1801 cattgccctg gaataatagt ctattgatta ttggcggtga aactgcaggc ggcaaagcgg
     1861 tgacggattc agttttgatc tctgtgaagg ataataaagt cacagtacaa aattaacgct
     1921 tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttcaattacg cgaaatttat
     1981 atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg tcagtctaat
     2041 gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc tcatcccaga
     2101 attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgtcatg tcactttaac
     2161 gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat
     2221 cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca
     2281 cggaaattac taccctttat tcctgataat gcaggggttc tcatcgttcc atgttgccgt
     2341 ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca cggagccagc
     2401 catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt cagcagaacg
     2461 cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc
     2521 gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatgatt
     2581 gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat tactgacgcg
     2641 ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttccctca tgcgtatgaa
     2701 gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag
     2761 caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga tttaagcacg
     2821 ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt
     2881 catttcagcg cggcagcccg tcgggggatt atttctgaca agtttgtaga agcaattttg
     2941 cagttttggc gcgaaaagtg agcgttcatt aatttggttt attcgcatcc atcagcgctt
     3001 ttaactcctc cggcgtaaac ggtggtttgc gccggtgcag catcgcatgg caattagggt
     3061 acacaggcag caaaaaaaca aatgagatca atgtaatata tcttcacttg caggcttgaa
     3121 cagcagaaaa tttgcgagca tgatcgcaat tatttcactg ccagggatat caatggcttc
     3181 cagtaaatcg ctacagcagg caattgccaa tataaaaatc tggcataagg gtgaacagcg
     3241 cgcaccgcat aaaccattgt tattgctata tgtattagcg ggatacctga atggacatcc
     3301 acgccttttc gattatggat cggaaatcta cgaacctctg cacagtttac tggaacgttt
     3361 tgggccacag cgttcacagt accgacctga tatgccgttc tggcgattac aaggcgacgg
     3421 attctggcaa ttacacaatg ccgagctttg ctcaacagca gggagcagcc gacaaccgcc
     3481 agtgaaggaa ctgactgaat accacgttgc aggcggtttc gatgaacagc actacgccct
     3541 ggtaaccggc aataaaaaac tcatcaatac tctggcgcaa cagatactcg aagcgcactt
     3601 caccgagagt attcaggaag aaattgctga cgagctgggt tttgatctcc agcaaatccg
     3661 taaacagcgc gacccgttat ttcgcaaaaa tgtattacgc gcttataact accagtgcgc
     3721 gatatgtggc tttaatatgc gccacgacga caccaccgtc gcgctggaag cggcgcatat
     3781 aaaatggaaa cagcacggcg gtccttgtga aattcctaat ggcctggcgc tttgcgcaat
     3841 tcaccataaa gcctttgata aaggatcgat tggcctggac gaaaatatgc gggtactggt
     3901 atcagatgcg gtaaacggcg gagggatcgt tgagagatta ttctgggatt ttgacgggaa
     3961 aactattgcg ttgccgcagg tgcgtagaaa ttacccttat gaggggtttg tggagtggca
     4021 tcggaaagag gtttttaggg ggtgaggtag gctatcttgt gcatacaaat agacaaataa
     4081 attgtataaa gagatagcct acaaatacta aataaattca gccccactag gaaaacgcaa
     4141 ctctgatgtc tgtaaactct cgccaataaa gaaaggtaca acccataaac gtaacctatt
     4201 ttccttctga tggctaaatt cataaggatg aggagatggc tgattaaatc cgttatgata
     4261 ggggtatatc aaaatcatat caccttcccc ctcctggtaa ttcaatccat aggcaagcat
     4321 ttggtagaga tcggcttgag attgttcaag actgtccttt ctgccattta ctatcttcca
     4381 tttaatatca caggtaataa ctgaaccagt agtactttta cgaggccgta aacaaatatc
     4441 aggccgcagc ttaaataaag ctttaccgtt ataactaatc agattttttg aacttacctg
     4501 tgcgtccact cgccatttat cataataacg ataacgcatc catgtcgtca caaaagattc
     4561 aaaaacagcc tccataggga aaagcagtga aaaagcatta acatccccat acatacagtg
     4621 tggagaatcg cccaataaaa tcatcttcgc ccacgctaat gcttgctcat agtgatgcat
     4681 gtgtctatca aagtgtaact gctcgaaagc tatataagca tcatgaactg acgtgatatt
     4741 ttcaaagtga aactgcagct gtgttatctg ctgtaataat tgtggattac tggtctggcg
     4801 actaatctta ttgattgcag ttttgagtat ccggttttcc agacgattga cgctgtactc
     4861 gtcatactcc acctgaaaaa gatcccgcct aatacagttt ttgcttaatt gtgcagagat
     4921 ccgtaatctt ccttttatcc atggaagatt atcttcctga cgtaaatagt cacgttttaa
     4981 ccctctctgc acaatcttac gcacgctctc tataaattgt tgaataaaga tatccatcag
     5041 aggcatttta gatattttga cgccagattc agaggaggca atatgacgaa atgattttaa
     5101 cgtacgtaac atcatcagta attgttgacg aacagtatcc tcattatcct gacctgtttt
     5161 aggcaggatt tcgagaaatc gcccccctgg cagtgagatt actcctgcat agttttttac
     5221 ctgcagcgtt tttactccat tccggctgct aagggtgagc aaacgatgag tattatcagc
     5281 atcgtctttc agacagcgca gctcaagcca tttaaatagg tctgcaggta gcactaccgc
     5341 attatttata cgggcatcac cttcctcact cactaaatgc ccgtattcga acacagtgaa
     5401 atgactggaa gatttcattc cgcactcctg cgtttttctt tttgaggtgc ataaatttgc
     5461 tgccaggcga ttggattgtc ccaaacgttg tcatcctctg cagccaggtg aaaactggtt
     5521 cccaagtcat ctgttccgtt atcaccaaag agatcggcat actttttctg gtatttcacc
     5581 tcacgtacaa attgaagatc tggtgcttcc tctttttggt tatcagccag taccatgcga
     5641 attttttgcc agtcattgta aaaatattct tccagcaatg gtagtatctt atttcgcata
     5701 atccgtttga atctttcaaa tgccagagct tcgtcttctt ttgcctgcac aacagggata
     5761 aaaaaagcat gccccagcat atgttctcta tcgtataaaa cctggatgcg atcgttaagc
     5821 gtctgcaata gacgaggcag atcaatacct ttaatgacag tatctttcag aacagtaata
     5881 tctggcagca tcgcttcaaa atcaaatctg cgacgtagtg cagtatcaag tgcagtaagc
     5941 gaatgatcag cagtgttcat ggtaccaatt aaataaaggt tattcggtac actaaatgga
     6001 gtggaagagt atggcagcaa tacgctaagc gattctggct caccagcccg tttagatggc
     6061 tcaataaggg taattagttc accaaaaatt tttgaaatat taccccgatt tatttcatca
     6121 ataatgagaa catagttttc ctttttttct gaagctacgc tctgagtatt cagaggcaga
     6181 taacgttgat taattaaata gttaaggata ctgcgtacat aagatggatt atgtactttt
     6241 gctcgtggat cctgataaac ttgccgaata tccttcaaat aggcattgta accctgtcct
     6301 aaatcctctt ttttcgattg tgacgggaaa attccaaagt tggtttgact tttatacgtc
     6361 agctggaagg ccttaccctg ttgtgtttcc agcgtgatat tttcaccttc agataaacgt
     6421 tcttgtaggc gagtaagtgc gctatccaac atttcctgcg caccagcatg accaaaggcg
     6481 gcatcctcgc atatctgttt aaaaatacct gcctttattt cgtattttac attaccgtca
     6541 tcagtagtct cagctcttaa accttcaata aactcttcat aaccaaagct ctgatgaaag
     6601 gtgataaaac gcactcgatt atctttgata agttgatcat aaatcttttt caactcgcga
     6661 cgtttttcac tctcatcctt accctcctgc agggagtaag cgatcggatc acaaatttgt
     6721 acagcaatct caatagtacg atatgtttta cctgtacctg gcgggccata gagaattgtg
     6781 tttttaggca atggcgaact tataacatca tcttcttgct cattttcttg taaagcactt
     6841 tcgcttccaa ctaaaccgat agcattttta tcctgaattt catacgcata gagatgccaa
     6901 agtgacatat tcagctcaat agggtcgata tcatcaccca gcgcacgatt tagcgtttct
     6961 ttaagatcaa cattgcgagc cagccactcc cgttctccgg aaagacctag ctggaaatgg
     7021 ttgttgcaat aattgtagca actaaaaaaa ttattaacat tcacgacact gctaattttt
     7081 gatgggtata ttgctgcaaa agcacgatta caaagagccc tatatacctt atttaatact
     7141 ccgtccgctt taagtttgat aaggcgttca atgacccgat tataattttc ttgtgtacac
     7201 tcttctgcta tctgacgggt gagcgttaga aggtcttttt gtgcattttc aaattcaacc
     7261 aatgacatcc ctccttgcat caggcttgag acaccattgt cacgttcata ccatacctgc
     7321 cgaatcgtgg cttcagaaaa gtttggtttt tcggcctgaa tctgttctac aagctggcgg
     7381 tagtgtttgc cccaaactga attgtattga ttattttttt caatttgagc acgaaagaag
     7441 ggggctaatg ctgatgaatc ctgaaccatc tcttaatcca atatgtaggg gggagggaaa
     7501 ttgggatagc cttatcttat cggattaatc tcaaaaatgt taaattttgt gataaaaaaa
     7561 ataactttca tcctgaaatt accttctgcc ctggagccac cacatttatc tgatgactta
     7621 actgcaaaac gcatgatgtt gatggctctc agagaaggct atagcattct ttcatacgta
     7681 aaatcgctac tcgtttttca acctgtgtga ggtttctagt tgcataggga atcaactgtt
     7741 tttaggtaaa
//
LOCUS       DAZFFI010000176         7360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFFI010000176 DAZFFI010000000
VERSION     DAZFFI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.178"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(323..1459)
                     /locus_tag="ACHJ12_004641"
     CDS             complement(323..1459)
                     /locus_tag="ACHJ12_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4992320.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1462..1824)
                     /locus_tag="ACHJ12_004642"
     CDS             complement(1462..1824)
                     /locus_tag="ACHJ12_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4992321.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2361..4274
                     /gene="mtlA"
                     /locus_tag="ACHJ12_004643"
     CDS             2361..4274
                     /gene="mtlA"
                     /locus_tag="ACHJ12_004643"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4992322.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4504..5652
                     /gene="mtlD"
                     /locus_tag="ACHJ12_004644"
     CDS             4504..5652
                     /gene="mtlD"
                     /locus_tag="ACHJ12_004644"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4992323.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5652..6239
                     /gene="mtlR"
                     /locus_tag="ACHJ12_004645"
     CDS             5652..6239
                     /gene="mtlR"
                     /locus_tag="ACHJ12_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4992324.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6251..6460)
                     /gene="yibT"
                     /locus_tag="ACHJ12_004646"
     CDS             complement(6251..6460)
                     /gene="yibT"
                     /locus_tag="ACHJ12_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4992325.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6745..7107
                     /locus_tag="ACHJ12_004647"
     CDS             6745..7107
                     /locus_tag="ACHJ12_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4992326.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1877 a         1839 c         1852 g         1792 t
ORIGIN      
        1 tctatagatt aaatattttt taaatgtgac tgtcatcctt cagataataa agccatcatc
       61 gcaatagatg gaaaagcact cctgctcttt tttgattaga gccgtcgcag gaggaacaac
      121 tcatatcatc attgcgatct cgacgatgta tagtctcatc ctgcggcaga acaagacgga
      181 taaaaaatcc aatgagagca cactcatcca gaacttatta acataatgaa tattaaagaa
      241 aaatcatcgc agctaatgaa gtaatatgca aaaataatta gtccttacaa ggagcaaagt
      301 tccataatct cccccctccc cctcaatgat ccaaataaag ataatgcatc cagctggtca
      361 ttcttagcag cactttccgc atcaccgaaa catggctgaa atggtcggag taaaccgcca
      421 cctgggcgta gatgccgtcg ggtaaggcat cgatatcatc gttagggtcc agttcaatgg
      481 ttcccagcac accgtccgtg ccgggcacga ccgttaatga ttgcaatacc ccctgcgcct
      541 gataagaacc gcctggcacg acaggtaaaa tactagtcag tttgccgtga aacacctgcc
      601 caggtagcgc gttaaacacc acttccgcat catcgccggg tttcagacgt aacagcgagt
      661 tttgccgaaa ttgggcgaca atttgccgtt tttgctcggg gatgaagacc atcaccggac
      721 gcagcggcaa ggcagctgcg tatgtacctg ggcggatcag tacctgagta acgtagccat
      781 tgctcggcgc gcgaatgaca gtctgctcaa ggttatattt tgcttcagta agttgcgctc
      841 ttaagctcac aatctgcgat tgctcgccgt taaccatact gtcgagctgg ctctggatct
      901 gcgcctgctc cgccaccgag cctttcacca gcgcatcctg cgcgaggaaa ttttgccgcg
      961 catcgtcgat gtcacgttcc gagaacggat tcaccgccgc ctggctgcct ttcaagtaac
     1021 gttgataatt tttaaacaga cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg
     1081 cttcagtgag ctgcgcacgc agcgtcttta tattatgcgt cgccgtcatc aggtcagcct
     1141 gaagtctgtc aactcgcgcc tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct
     1201 tttgaataag ctgattattc ttgtcagtga cttcagtaac aattcccgtc acctgtggcg
     1261 tgatggggat cgctatcact gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca
     1321 tcaacaaaat caaaccactc accagaaaca cgccacccag cgccgccgtc gccagcgtcc
     1381 actgattcac cggaatacga aagattttaa agaccgccca cgccagcgcc acgtaagtta
     1441 aaacaatcaa tagatccata attagatctc cgggaacgtg gaatagtcag tctttttctc
     1501 ggcggcgagt tgatgctcca gccgggaaat tcggtcagaa agtgcggcga tttccgggtc
     1561 agtcgctttc tcctgcgacg caacatgtga ctgcataccc cagccacgct ccggttgata
     1621 gagcgtcgcc cagatccaca gaaacggcca gataacatgc agagtaaaca ggctcaccca
     1681 gcccgccgta tgaatagcgt cggcatgggg atggttgcgc tttttggcaa tcagataagg
     1741 gatgtcatgt atcgcgatga ttccataaaa aatcaccaga aagacgaaga tcagcactcc
     1801 caacgcgaaa tagtttagaa acatatctgc ctcggattca cgtttatcag tgttgttttt
     1861 gggctggcag ccagaaggga gtcaggctga tattttgaca ataatccggg ttcgcgattc
     1921 tcgccataac accaaagaat aatttttaga ggtgatgagt tgcttagtta cataacgatt
     1981 gtatgacgaa ggcataacat gctgtagatc acatcaggtg aacgccgtaa gaaaatatct
     2041 tgtgattcag atcacaaaga ttcaacaaac catcaaaaca aaaatgtgac actactcaca
     2101 tttaaatgcc atttttagcg aaaatcgccg tcttgttgct tttttacaca agcgttttgt
     2161 gatgagtatc acgtcaatta cctctctacc ccctatattt atgtgactga tatcacacaa
     2221 aaggccgtcg actggacagt taaccgattc agtgccagat ttcgcagtat ctacaaggtc
     2281 cggctacctc tgccgccaca ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa
     2341 acataagaag gggtgttttt atgtcatccg atattaagat caaagtgcaa agctttggtc
     2401 gtttcctcag caacatggtg atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca
     2461 ccgcgttatt tattccaaca gggtggttac cgaacgagac gctggcgaag ctggtcgggc
     2521 cgatgatcac ttatctcctg ccgctgctga tcggttatac cggtggtaag ctggtaggcg
     2581 gcgaacgtgg cggcgtagtc ggtgccatca ctaccatggg cgttatcgtc ggcgcagaca
     2641 tgccgatgtt cctcggttct atgattgcag gtccgctggg cggctggtgc attaagcact
     2701 tcgaccgctg ggtagacggt aagatcaaat ccggttttga gatgctggtg aataacttct
     2761 ccgcaggcat catcgggatg atcctcgcca ttctggcatt tctcggcatt ggcccgattg
     2821 ttgaagccct gtccaaaatg ctggctgcgg gcgttaactt catggttgtc catgacatgc
     2881 tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct gttcctcaac aacgccatta
     2941 accacggtat cttctcgccg ctgggtattc agcagtccca tgaactgggt aaatccatct
     3001 tcttcctgat tgaagctaac ccaggtccag gtatgggcgt gctgctggcg tacatgttct
     3061 ttggtcgtgg tagtgctaaa cagtctgcgg gcggtgcggc aatcatccac ttcctggggg
     3121 gtatccacga aatctacttc ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca
     3181 tcctcggcgg tatgactggc gtgttcacgc tgactatcct gggcggtggt ctggtttctc
     3241 cggcatctcc gggttctatc cttgctgtac tggcgatgac accaaaaggt gcttatttcg
     3301 ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt cgttgtctct gctattttgc
     3361 tgaaaaccag caaagtgaaa gaagaagatg atattgaagc agcaactcgt cgtatgcagg
     3421 acatgaaagc tgagtctaaa ggcgcatctc cgctgtctgc tggcgatgtg actaacgacc
     3481 tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg tatgggttcc agtgcgatgg
     3541 gcgcaggcgt tctgcgtaag aaaattcagg atgcaggtct gtcgcagatt tctgttacta
     3601 acagcgcgat caacaacctg ccgccagatg tggacctcgt catcactcac cgtgacctga
     3661 ccgaacgcgc tatgcgccag gttccgcagg cacagcatat ttcgctgacc aacttcctcg
     3721 acagcggcct gtacaccagc ctgaccgaac gtctggttgc tgcccaacgc cacacggcaa
     3781 acgaagagaa agtaaaagac agcctgaaag acagctttga cgattccagt gctaacctgt
     3841 tcaagctagg cgcggagaac atcttcctcg gtcgcaaagc ggcaaccaaa gaagaagcga
     3901 ttcgttttgc tggcgagcag ctggtgaaag gcggttacgt tgagccggaa tacgttcagg
     3961 cgatgctgga tcgtgaaaaa ctgaccccga cttatctggg tgagtctatc gcggtgccac
     4021 acggtacggt tgaagcgaaa gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc
     4081 cggaaggcgt gcgcttcggt gaagaagaag atgacattgc ccgtctggtg attggtattg
     4141 cagcccgtaa caacgagcac attcaggtta tcaccagcct gaccaatgca ctggatgatg
     4201 agtccgtcat cgagcgtctg gcacacacca ccagcgtgga tgaagtgctg gaactgttgg
     4261 caggtcgtaa gtaatccaat cccaccctct ccacatggag aaggtggggt taattgcctg
     4321 atgcgctacg cttatcaggc ctacaggatg catcacgatt tgttgaattt gcacgttctt
     4381 gtaggccgga taaggcgctt acgccgcatc cggcgctgcc cctctcctca cggagagggt
     4441 ttgggtgagg gaaaagcctc accccagccc tctcgggtaa aaacattgat gaaggttaat
     4501 actatgaaag cattacattt tggcgcaggt aatatcggtc gtggctttat cggtaaactg
     4561 ctggcagacg cgggtatcca actgacgttt gccgatgtca atcaggtggt acttgatgcc
     4621 ctgaatgccc gtcatagcta tcaggttcat gtggttggtg aaaccgagca ggtagatacc
     4681 gtttccggcg tcaatgctgt cagcagcatt ggtgatgatg tcgttgatct gattgctcag
     4741 gtcgatttag tcactaccgc cgttggcccg gttgtgctgg aacgtattgc tccggctatc
     4801 gccaaagggc tggtgaaacg caaagaacaa ggtaatgaat ccccgttgaa catcatcgcc
     4861 tgtgaaaaca tggtacgcgg taccacgcag ctgaaaggcc atgtgatgaa cgccctgccg
     4921 gaagacgcca aagcgtgggt agaagaacac gttggctttg tcgattccgc cgttgaccgc
     4981 atcgtaccgc cttcggcttc ggcaactaac gatccgctgg aagtgacggt agaaactttc
     5041 agcgaatgga ttgtcgataa aacgcagttc aaaggcgcac tgccgaacat cccaggcatg
     5101 gagttaaccg acaacctgat ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt
     5161 catgctataa ccgcgtacct cggaaaactg gccggtcatc agaccattcg tgacgctatt
     5221 ctcgacgaga aaatccgcgc ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg
     5281 atcaagcgct acggctttga cgcagacaaa catgcggcgt acatccagaa aatcctcggt
     5341 cgttttgaga acccgtatct gaaagatgat gtagagcgcg taggccgtca gccgctgcgt
     5401 aaactgagtg ctggcgaccg tctgatcaag ccattgctcg gtacgctgga atacagcctt
     5461 ccgcacaaga atctgattca ggggattgct ggtgcaatgc acttccgcag tgaagatgat
     5521 ccgcaggctc aggaactggc agcactgatc gctgacaaag gtccgcaggc ggcgctggca
     5581 cagatttccg gtcttgatgc caacagcgag gttgtatccg aggcggtaac cgcttataaa
     5641 gcaatgcaat aatggtggac caggcgcagg acaccctgcg cccgaataac agattgtcag
     5701 atatgcaggc aacaatggaa caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga
     5761 atgctggcaa aaccgtgcga agctttctga tcaccgccgt cgagcttttg accgaggcgg
     5821 taaatcttct ggtgcttcag gtattccgca aagacgatta cgcggtgaag tatgctgtag
     5881 aaccgttact cgacggcgac ggtccgctgg gcgatctttc tgtgcgttta aaactcattt
     5941 acgggttggg cgtcattaac cgccaggaat acgaagatgc ggagctgctg atggcattgc
     6001 gtgaagaact aaatcacgac ggcaacgagt acgcctttac cgacgacgag atccttggac
     6061 cctttggtga actgcactgc gtggcggcgt taccaccgcc gccacagttt gaaccagcag
     6121 actccagttt gtatgcaatg caaattcagc gctatcaaca ggctgtgcga tcaacaatgg
     6181 tcctttcact gactgagctg atttccagaa tcagcttaaa aaaagccttt caaaagtaag
     6241 caacgtctgc ttactgcccc tctacctgct tcggccgata aagccgacga taatactcca
     6301 gacgttgtag atataacggc acgctttcat cgggtattcc ggacggaatc gcgttacggg
     6361 gaggaagttt tttcagatac tcccggaacg cctggcttga tgccatgaaa tctacggctt
     6421 tatcgataag aagcggaacg ttttcaccta atttgcccat gatgatatct ccgtattacc
     6481 ccgcgccggg aatgcgcggc cgccaatttt agtttaggtt cccggtaaag atgacattaa
     6541 ggaaaacgtg ctgaatcctc aataatcatc cgctgctgtt tattatcttt tttctataga
     6601 tttcttaatt aatcaataaa ttgcgtagtt tttaagcatg ttaatgatta cgttacagaa
     6661 ctattgcaat gtcatcagtt gtgaacaaag cacctggtcg cgcatactag gggctataaa
     6721 tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc
     6781 agcgatcgtc tggacgccat ccgccaccag caggccgatc tgtcgctggt tgaagccgca
     6841 gacaaatatg ccgagctgga aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg
     6901 cgtgaagttc atagccaaaa actgagtaaa gaagcacaga agctgatgaa gatgccgttt
     6961 cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca agctgaagaa aagtgttcgc
     7021 ggactggtgg ttgtgcaccc aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg
     7081 actggattct ctaagactgc gttttaagcg cctcagacga ctggttcgat catatcaacc
     7141 cgcatctggc ttgccttagc tggtcaatgc ggggactctc ctgtctactc gcctaattac
     7201 caaacaattc actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt
     7261 taccgtaata gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac
     7321 aagcatttac gtctcttttt ttcggattta attgtgaaat
//
LOCUS       DAZFFI010000177         7340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFFI010000177 DAZFFI010000000
VERSION     DAZFFI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.179"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(150..434)
                     /locus_tag="ACHJ12_004648"
     CDS             complement(150..434)
                     /locus_tag="ACHJ12_004648"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992327.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(855..1868)
                     /locus_tag="ACHJ12_004649"
     CDS             complement(855..1868)
                     /locus_tag="ACHJ12_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO4992328.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(1880..3196)
                     /gene="fucP"
                     /locus_tag="ACHJ12_004650"
     CDS             complement(1880..3196)
                     /gene="fucP"
                     /locus_tag="ACHJ12_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4992329.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            complement(3224..4144)
                     /gene="rbsK"
                     /locus_tag="ACHJ12_004651"
     CDS             complement(3224..4144)
                     /gene="rbsK"
                     /locus_tag="ACHJ12_004651"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4992330.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            4449..5231
                     /locus_tag="ACHJ12_004652"
     CDS             4449..5231
                     /locus_tag="ACHJ12_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO4992331.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISDRPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(5233..5459)
                     /locus_tag="ACHJ12_004653"
                     /pseudo
     CDS             complement(5233..5459)
                     /locus_tag="ACHJ12_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(6139..6276)
                     /locus_tag="ACHJ12_004654"
     CDS             complement(6139..6276)
                     /locus_tag="ACHJ12_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992332.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTRVNIDD
                     Y"
     gene            complement(6626..7192)
                     /locus_tag="ACHJ12_004655"
     CDS             complement(6626..7192)
                     /locus_tag="ACHJ12_004655"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4992333.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GLNKAIPSLMADFTGELHRLISEYLQCKRRRDKSVEPTPLYYLWSRETVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
BASE COUNT         2142 a         1717 c         1556 g         1925 t
ORIGIN      
        1 tcccattttt ctttcttcca gtcaggactg catccgatta atcgtaagcg ctccgggaag
       61 tacgtagcgt aaggattatt ttacagctga gaagtttcag tgcagcagtt catgcacacg
      121 gttcgacggc tagtcattga tcttcataat cacctcgcgt aaaaagcggg gtaaggccgc
      181 tcatccagcg tcatctccac aggtaataat tcaacgccaa tgccagaaga gttccttacc
      241 ggaagacgcc gtgatatacg ccctttgatc agccagagcc tgagccattt gaacagaaga
      301 ttatcattga tatcatgctt ccgggcaata cgggcaacag aaacccttgg ttgtaaagcc
      361 agtttaacca tttgaagttt aaactcattt gaaaatgacc tgcggggttc agctgataat
      421 gttttttgtt ccataacagg tgtccactag ttgaaaaggt gggtacctat gttaccaata
      481 ctggcttaat ggctacatac agcagtcggt ttacgcatac acttttatag tgcactccgg
      541 cctcatgaat ataacggtgg attaccgccg aacgaatcgg aaaatagata ctggaaaaat
      601 ctgaaattgt gaccagtttt tgttgaccac tacaaataaa taaaagagtt gcagtgcttg
      661 ctcggtaaga aaacaatgga agttgagatc ctaaaagaag ctgtggagta caccactcta
      721 cagtagttgc gcaaatttgg agtggacgat aatgtgatcg tctataaggg caacgctatc
      781 atagtcttgt cctggcgggt aaaaaaacgc gcttacctta acgataagcg cgccgctgtt
      841 caggccttaa gtggttattc aattcctgtg gtgactgtaa aagtgcgcgt ttgctgcggt
      901 gcaacctgaa tcagcgtgcc attacgttgc gcggcaagat atccctcagg ccgacaggtt
      961 gcaggtaatg caaaggcggc tacctgttgc tcgccgttat aaaggatcca gcgtgtcaca
     1021 taatttagtt cggcactgga gaaacgagta acaaacgtag tgccatcggg agcgatcatg
     1081 cgaaactctg gctgatctgt gtaagcgtcc agtttgtctg caaagaagac aatttctgga
     1141 tcataaaact ccggttgatt cagcgtcgac agtgaggctt ctccctgcat aatccgttga
     1201 ttaaacgcca gccactgagt ggtgggatta acatgcgaag gtactgattc acgcaacctt
     1261 aatatttcgt ccggtatatt ctgactgaac gtagcatttg gtatatatgc ataattcatg
     1321 tggcacatat attgtagtgg catatctaca gaagccagat tggttacggc catcttaata
     1381 tcgaacagtg tagaggattt gtgaaggacc actgttggct gagccagata atgatgcccg
     1441 aaccccttta catactcgta acgcccggta aggcgtaaca tatctccctc taattccatc
     1501 catgcttcat ccatcgcggc acaggccatt tcaccgtgta gcagatgagt atcttccgca
     1561 gatgggcagc cattagccag caaacctgaa tgaaaggcaa aacagccata ggtctctatc
     1621 acctctgtcg ccggtttagg ctggcgaaac atattgcaca tggtgagact gtgtccatca
     1681 aattgcgcat cccaaatcat ctgccccatc cagggaagaa taattaaatg tccacgactg
     1741 tttgcaattt taagcccctc gacaccactg tcatagcgaa aagacgtgac agtaaaatca
     1801 ctattttcca gcaagatacg aggtttttcg ccaaaaagcg cccgccacaa ataaatacgc
     1861 gtactcataa cgattctcct caggactctg tgacttcagc cagtgcatta cgtactttgc
     1921 tttcacgcca gaagtagaca ccgacataga caaagcagag catagaaacc aagaatgaaa
     1981 gctgtagtga gtggaacata tctgcaatat atccctgaat tgccggaacc accgctgcgc
     2041 caacaatagc cataacaatg actgctcctg ccatttctgt atgttggtta tcaactgtat
     2101 ccagtgttcc tgcatagatc gtcgcccagc aagggccaaa caaaacactt accaggacgg
     2161 cgacatagac cgcactgaaa cttggagcca gtgcaacata tgccagaaac agcgccccta
     2221 taacggaata gagaatcagg actttttccg gattaaaacg tgtcataagg atgttggcta
     2281 taaacttgcc aataaagaag caggcaaagc tgtagaccat gaagtttgaa gcatcacgtt
     2341 cgttgatatc gcccaactcc agcgccagac ggatggtaaa tgaccatact gcgacctgca
     2401 tacccacata aaggaattga gccacaatac cacgacgaaa gcgcggattt ctagccagat
     2461 agcgcagcgt atccattgct gacgggcgtt tatggtgact tgtctgtgcc actttacagg
     2521 ttgggaagcg ggttaaaagg aacaacacca tgaccacaac cagaatcata atcatatact
     2581 tatacggttc aagggtgttc tctaacatca gcaccttaaa gttgtgaatt tgcccggcgt
     2641 tcattccgga catctgcttc tcaaggcttt ccccctcgga gaaaaccaga tatttgccca
     2701 ataaaatacc agacgcagca ccaatcggat aaaaggtctg gctgatattg agccgcaatg
     2761 tggcgtaggc ttttggacca atcatcgaac tgtatgtgtt cgctgcagtt tcaaggaagc
     2821 tcaggccaat cgcaatcgca aaaatagccg caaggaacat agtgtaggtt gccatatgcg
     2881 aggcagggaa aaaaagtgta caaccaccaa tatacagcgt caggccaatt aaaattgcca
     2941 ccttataact ggtcttttta atcacaaggg atgctggtat tgcaattaaa aaataaccgc
     3001 cataaaatgc gctctgcacc aatgctgaag caaagttact tagcgaaaat acacttttga
     3061 attgagtgat taatatgtca tttaatgcag ctgcgcatcc ccataacggg aataaacacg
     3121 ataacaaaat aaactggaac aagggagtct tattcagata cccatcaggc atctgaatga
     3181 tgtttttatc gttcatagtg ctacctttaa ctgtacagga tgattattcg tttaaggtta
     3241 aaaattcatt aaattgttca atgctcggat aagatgattg cgtacctttc cctgtgacgc
     3301 tgaaagcggc aaagagagcg gcttttttca aagcggcttc aacatcaccg ctttgaacat
     3361 aataatggga aaagcaacca ataaatgcat caccagcgcc actagtatca acagcattta
     3421 ctttgaatgc aggaacatgg acttcctgat cgcgggtcat ccataatgcg cctttttcgc
     3481 tcatggtaac aataatattg ttcagccctt tatcaactaa cgaacgcgca gccaaacgaa
     3541 tatgatcata agtatcaacc gacatacctg ttaatatttc cagttctgtt tcattcggga
     3601 taaagaaatc acatttgcag gcataagaca tatctaactc acgcaatgcc ggagccggat
     3661 ttaataacac ttcaatacca tttttcttac caaactcaat cgcgtggtaa actgtttcca
     3721 gttgaacttc cagttgtaaa acgatcaatt tgcatttttt cagatcttct gcagctcgat
     3781 cgatatcttc cggggaaaga aatttattcg cccccttaat tattaaaata ctattgctcg
     3841 agttagcatt aacaaagatc ggcgcaacac cactgctggt acaggggact ttctcaacat
     3901 aagtggtatt aattccccaa gattcaagat tacgaatagt attatccgca aaaatatcat
     3961 cacctacttt agtcagcatc aggacttttg aattcaattt agccgccgcc accgcttgat
     4021 tagcaccttt cccaccacat ccgattttga aggcaggtgc ttccagagtt tctccttctt
     4081 taggcatctg attagtgtaa gtaatgagat ccaccatatt ggaaccaata actgcaatgt
     4141 ccatttcact acctcttata aactttcgca taacaatggt atttaaataa cattatcatg
     4201 ttacttttgc atcatttgtg actgagatcg cgattagcac atcaacccga tgtttattta
     4261 atagacttcc agtcttatca ctcaggccaa cactatctaa tcataagcaa cctaacagga
     4321 ttaataccga aaattcagca ggctataccc ttttcatttc aaagggtcgg tcgtatagta
     4381 tggtaactaa aacaatgttt actaatgcca tgatgttatt tttataacat ttttcggagg
     4441 aagaattgat ggaaacgaag caaaaagagc gtatccgacg tttgattgaa atacttaaga
     4501 aaaccgacag aatccatttg aaagacgcgg cacgaatgct ggaagtttct gtaatgacta
     4561 ttcgtcgcga tctccatcag gaagatgaac ctctgccact gaccctactg ggtggctata
     4621 ttgtaatggt gcataaaccc gcaccatcca tgccagtaat ccaggacgtt ccgagaaatc
     4681 atcgtgatga cttacctatt gcaattctgg ccgccggaat ggttaatgaa aatgatctga
     4741 tcttctttga taatggccag gagataccgc tcgttataag catgatcccg gatgcaatca
     4801 ccttcactgg catctgttac tcacatcgtg tctttgttgc gttgaatgaa aaacctaatg
     4861 tgacagcaat actttgtggt ggtacgtatc gtgccagaag tgatgctttt tacgatgcca
     4921 gtaactcttc gccattagac tctctcaatc cgcgaaaaat atttatttcc gccagcggtg
     4981 tacatgatca ctttggcgtc agctggttta atcccgaaga tcttgccact aagcgtaaag
     5041 cgatggcccg tggactacgg aaaattttgc tcgcccgcca cgccttgttt gatgaagtag
     5101 cctctgccag cctcgcaccg ctctctgcat ttgatgttct gattagtgat cgtccgttac
     5161 cggcagatta tgttacgcac tgccggaatg cttctgtaaa gatcattaca cctgattcag
     5221 aagacgaatg acttactgaa aaaacaccgt actcttgtta aacatcgtcg gattggactg
     5281 attacgttgc actttcatca catattccag cttatcaatt tggcttatca tctactccag
     5341 acgctggtca tcattgacca atagtctgat atggcttttg tcgcggtcct gaatcggcag
     5401 gcagaaaatg acttcaacgt taaaagggcg acgggaaaaa gcccacaaag gtgagtcatt
     5461 acgcccggat aataaacagg ctcctccctg ttatggaatc cgttgtattg tccataatgt
     5521 ctgggttttt aatgcacact ggataacaac cggacgaatc ttcaaatatc actggcgctg
     5581 acagccggtt tgaattcatc tcacaaccct gcatagagcg aatctcctgc ctgcgcgtca
     5641 ctccactcca tggtatcaac ttcacactct ttatctgcgg ctagtttcag tcaccagata
     5701 agcatcgact atgaaaggtg agccatgaca acataacgtt ggtaacgctc tgatgcctta
     5761 acggaagatg cctgccacca cagggaatgt aaacgactga agtgtggcct ttaatgccgt
     5821 gaacggctca tggtctcctg gcacggttgc cgccccaacc tgtaacaaca ttccacagta
     5881 caatgtctgt cagagccgta gcctcccatg cctgttgtag taactctacc agttacgaac
     5941 atccttcctc aaaagtgttg tcgtatctcg catggaagaa aagatcctga ctaagcagca
     6001 acacacaacg tattgcggaa cctgcatgtt tttcctgtaa ctagtgtatt acgacatatg
     6061 gtaatagcta cctgtgtggt ttcgctggat aacaagggga tttattcgca agtaaaatgc
     6121 ctgataaaat acacgaatct agtaatcatc aatatttact ctggtcgaat gaagtgtgaa
     6181 gtggattgcc agcagatgcg gccagtgatc caccgcctgc tgaacaaaac gccggatttc
     6241 ccccggctct gaaagtaagg cttcggttat ttgcactatt ttatctctgt tgaatttggt
     6301 taagtcggtg cagactcatc aacacaagta cggttcgatg caaacagctg tgactggcaa
     6361 tatgaaagga atgatgaatc agtcaggatg acaaagtgcc ggctgaccgg aggggacgca
     6421 ggaagattca cggggggacc agcaccaggg aacagcgcca catcccgggt ggtactgctt
     6481 tgatgcgccc ggtgacacaa agcccggatt gttccagaca tcctgaatca aacgtcccag
     6541 attaggggcg tcgaaatatg cctctctgac cattatattc cggcgtacag gtagcaggtc
     6601 agaagtgaca atgcgtcacc tgacgttaaa agtcactaca cccaagatga cgttcaacag
     6661 caccatgcga ttcaatgtaa gcccgggctg tctgttccag tacaccaggc tcagcgttgt
     6721 atgtgttagc tgcatcaaat accaacgaca gcacttcagg atacacaacc agatgtgtaa
     6781 tggagttatc ttcacccaat acttttcccc acgcctgctc aatcagattt ctgagaacca
     6841 ccacctcacg actcttacac cagacatcgt tattaagtag cagcaccata agataaggag
     6901 tggtatcgtt agccacagtc tccctgctcc agagataata taaaggggtg ggctcaacag
     6961 atttatctct acgtcgctta cactgcaaat attcagaaat gagtctatgc agttcaccag
     7021 taaaatcagc catcagagag ggaatggcct tattaaggcc ctgccctggt attaatttaa
     7081 attgtaataa tttaatttca ggatgtgtgg ctgcagcccg atacagagtt gcaaggacac
     7141 acttttgcca gagggcgtta caggaaagct taacgtttga ttctgtatac ataataaatc
     7201 accttacagt tacaacaggt caaaaaccgc tgtagccaga gttacgctgg cctgatgctt
     7261 tagtaccggg cttcgtcaga taatccagac gctccaataa gcgctgatac tgctcaggga
     7321 aatcaggatc atgaatatcc
//
LOCUS       DAZFFI010000178         7113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFFI010000178 DAZFFI010000000
VERSION     DAZFFI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.180"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..685)
                     /locus_tag="ACHJ12_004656"
     CDS             complement(<1..685)
                     /locus_tag="ACHJ12_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO4992334.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAK"
     gene            complement(863..1774)
                     /gene="gsiD"
                     /locus_tag="ACHJ12_004657"
     CDS             complement(863..1774)
                     /gene="gsiD"
                     /locus_tag="ACHJ12_004657"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4992335.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1777..2697)
                     /gene="gsiC"
                     /locus_tag="ACHJ12_004658"
     CDS             complement(1777..2697)
                     /gene="gsiC"
                     /locus_tag="ACHJ12_004658"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4992336.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2715..4253)
                     /gene="gsiB"
                     /locus_tag="ACHJ12_004659"
     CDS             complement(2715..4253)
                     /gene="gsiB"
                     /locus_tag="ACHJ12_004659"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4992337.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4273..6144)
                     /gene="gsiA"
                     /locus_tag="ACHJ12_004660"
     CDS             complement(4273..6144)
                     /gene="gsiA"
                     /locus_tag="ACHJ12_004660"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4992338.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6131..7096)
                     /gene="iaaA"
                     /locus_tag="ACHJ12_004661"
     CDS             complement(6131..7096)
                     /gene="iaaA"
                     /locus_tag="ACHJ12_004661"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4992339.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVMEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         1791 a         2032 c         1665 g         1625 t
ORIGIN      
        1 tcttggcaat gatctcatga ccttgtttca aaaatagaac ttcatcatta gcgctgattt
       61 gatcaagata ctcgttcaga taccgccagt ttaaatcgac atgaatttta tctaccgcta
      121 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
      181 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      241 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      301 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      361 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      421 ttttcagaat cacggcacgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      481 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      541 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      601 tgaaaaaaag gccaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      661 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataactg tctgaacgct
      721 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      781 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      841 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      901 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      961 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
     1021 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
     1081 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aggatatgac
     1141 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1201 gtttcaacac cagcgtgttg ccgcgcacca ggcgggcaaa cgcggggatg gaaaaaatgg
     1261 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1321 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1381 agccttcata atatccagcc agcaagccca gcaacgtccc tatcgccgca ccgataaaca
     1441 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1501 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1561 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1621 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1681 gccagaattc atgccacggt gtacgtacct ggtcaggttt gaccagtggc atggcgttta
     1741 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1801 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gtgagaaaag
     1861 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1921 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1981 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttgcgtagcc cgtgtttgag
     2041 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     2101 aacgtcgaca aatgacgcac gggtaaagcg cgccatcacg gcggccactg ccgcgccgag
     2161 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2221 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2281 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2341 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2401 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2461 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2521 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcaggccc
     2581 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2641 gacgataaac agcgtcggaa tcaaccccag taatcgtttg ataacgtaat taagcattcc
     2701 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2761 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2821 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2881 gccggatcat tagttttcag tgcctgagcc aggaagtcat ccacctgttt attgctgtaa
     2941 aacgcggtat taaacagcgt cggtggccag ttttgcgagg caaacaacgg cgatagtgcc
     3001 cagtcggctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     3061 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc agtcacctgg
     3121 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3181 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3241 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3301 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3361 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3421 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3481 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3541 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3601 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3661 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3721 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3781 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttactgt tgttggatcg
     3841 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3901 tcgctggccc ggtccagatt cgctttcacc gccgcggcgt tgaaatcggt gccatcctgg
     3961 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     4021 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     4081 aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg
     4141 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4201 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc
     4261 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4321 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4381 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4441 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4501 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4561 ctctaccacg gccatgtcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4621 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4681 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4741 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4801 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4861 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4921 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4981 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     5041 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     5101 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5161 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5221 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5281 cgggaaacgt cggggataat ctaacccttt catcgcacca agttgcggaa cagcagctaa
     5341 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5401 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5461 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5521 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5581 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5641 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5701 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5761 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5821 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5881 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5941 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     6001 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     6061 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6121 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6181 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6241 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6301 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6361 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccggta caagaaaccg
     6421 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6481 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6541 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6601 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6661 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6721 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6781 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6841 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6901 acagtggaca ctcttccagc agacgcaccg cttccatcac cacatccagc gcactttcgc
     6961 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     7021 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     7081 tgactgcttt gcccatagta ttcgtccatt aag
//
LOCUS       DAZFFI010000179         7102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFFI010000179 DAZFFI010000000
VERSION     DAZFFI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.181"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            162..1496
                     /gene="adeQ"
                     /locus_tag="ACHJ12_004662"
                     /gene_synonym="yicO"
     CDS             162..1496
                     /gene="adeQ"
                     /locus_tag="ACHJ12_004662"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO4992340.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1549..2001
                     /locus_tag="ACHJ12_004663"
     CDS             1549..2001
                     /locus_tag="ACHJ12_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4992341.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2212..3402
                     /gene="nepI"
                     /locus_tag="ACHJ12_004664"
     CDS             2212..3402
                     /gene="nepI"
                     /locus_tag="ACHJ12_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4992342.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3443..3736)
                     /gene="yicS"
                     /locus_tag="ACHJ12_004665"
     CDS             complement(3443..3736)
                     /gene="yicS"
                     /locus_tag="ACHJ12_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4992343.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3958..4776
                     /gene="nlpA"
                     /locus_tag="ACHJ12_004666"
     CDS             3958..4776
                     /gene="nlpA"
                     /locus_tag="ACHJ12_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4992344.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4780..5703)
                     /locus_tag="ACHJ12_004667"
     CDS             complement(4780..5703)
                     /locus_tag="ACHJ12_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4992345.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5814..6998)
                     /locus_tag="ACHJ12_004668"
     CDS             complement(5814..6998)
                     /locus_tag="ACHJ12_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO4992346.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1784 a         1597 c         1787 g         1934 t
ORIGIN      
        1 tgattttatc aatttgatta ttatttatca taaagaaacg tcgcgagatt attaaagaat
       61 atccattaat gtgcaattga aatgtgataa ttatcactga accgttgttg tcatttgttt
      121 tacaaaagca agggattaaa tctcctccaa tggacaaaaa aatgaataat gacaataccg
      181 attacgtgag taatgaatca gggacgcttt cgcgattatt taaactacct cagcatggga
      241 ccaccgtccg cacagaattg attgcgggga tgaccacttt tttaaccatg gtgtacatcg
      301 tttttgtgaa cccgcaaatc ctcggcgcgg cacaaatgga cccgaaagtg gtgtttgtta
      361 ccacctgttt gattgccggt atcggcagta ttgcgatggg gatatttgct aacttacccg
      421 tggcgctggc tccggcaatg gggctgaacg ccttctttgc cttcgtggtc gtgggggcga
      481 tgggcatctc ctggcagacc gggatgggcg caatattctg gggcgcagtt ggactatttt
      541 tgctcacgct gtttcgtatc cggtactgga tgatctccaa cattccctta agtttacgta
      601 ttggtatcac cagcggaatt ggattattta tcgccttaat gggattaaaa aatactggcg
      661 ttattgtcgc caataaagac acgctggtga tgattggcga tttaagttct cacggcgtgt
      721 tgttaggtat tttagggttt tttattataa ccgtgttgtc atcacgtcat tttcatgccg
      781 cggtgctggt ttctattgtg gtgacgtctt gctgtggatt atttttcggt gatgttcatt
      841 ttagcggcgt ctattccatt ccgcctgata ttagcggcgt cattggtgaa gtagatttga
      901 gcggcgcgtt aacacttgaa ctcgccggta tcattttctc ctttatgctg atcaacctat
      961 ttgattcatc aggaacatta attggtgtaa ctgataaagc gggcttaata gatagtaacg
     1021 gtaaattccc caatatgaat aaggcgctgt atgttgatag cgtcagttcg gtggcggggg
     1081 cgtttatcgg cacctcgtct gttactgcct atattgaaag tacttctggt gtggcagtcg
     1141 gtggtcgcac ggggctgact gcggttgtgg ttggcgttat gttcctgtta gttatgttct
     1201 tctcaccgct ggtggcgatg gttcctcctt acgcaaccgc cggagcgtta atctttgttg
     1261 gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc gaatcggtgc
     1321 ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa gggattgcac
     1381 tcggctttat gtcgtactgc atcatgaaag tatgcaccgg gcgctggcgc gatctgaacc
     1441 tgtgtgtggt ggtggtcgca gctctgtttg cactgaagat tattctggtg gattagtaac
     1501 tggcgagggt ttccagatat catgagttct gattacgcag gagaactcat gatctggata
     1561 atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac atccggggcc
     1621 agaaaagcga ttcgccgtct cagcgatcgg ctgaacatcg atgtcgtacc cgtggagtcg
     1681 atggtcgatc aaatgggaaa gtcagctggt gacgaatttt tacgttattt gcatcgtccg
     1741 gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctca tctggcaaat tgtcattgtc
     1801 gatggtagcg aacagaacct gctgcaatgg catcggattt tacaaaaagc tcgcctcgcc
     1861 gcgccgatta ccgacgctca ggtcaggctg gcgctaggtt ttctgcgcga aaccgaacct
     1921 gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca gcctgccgag
     1981 ggcgttcact ggttgcattg aaaccgtttc gtcttatcag cctacagacc gcgcctgacc
     2041 tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt ccgtttcgat
     2101 cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaatgc gtcacacatt
     2161 tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac catgagtgaa
     2221 tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc agccgttttc
     2281 tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt cagtttgttg
     2341 acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc ggtgaccgtg
     2401 accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca ggctactgac
     2461 cgccgctacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt gctggtttcc
     2521 tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct ggcgctgggc
     2581 gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg tgccgccgcg tacggtgccg
     2641 aaggcactgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc cgcgccgttg
     2701 ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc ggcggtgatg
     2761 ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg cgaaccctcg
     2821 catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc agggatgatc
     2881 gccatcttca tgtctttcgc cgggcagttt gctttcttca cgtatattcg cccggtgtat
     2941 atgaacctgg cgggattcgg cgtggatggc ttaacgctgg tgctgttgag ttttggtatc
     3001 gccagcttta ttggtacgtc gctttcgtcg ttcattctta aacgttcggt aaaactggcc
     3061 ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tgctgacgtt gtggggaagc
     3121 gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc attggttccc
     3181 gtcggctggt caacgtggat cacccgctcg ctggccgatc aggcagaaaa agccgggtct
     3241 attcaggtgg cggttattca gcttgctaat acctgtggcg cggcaatcgg cggttatgcg
     3301 ctggataata ttggtctgac ttcgccgttg atgttgtccg gcacattgat gttgctgact
     3361 gcattgttgg ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt aaagaagcgt
     3421 tggtcagtag ttgttgctgt tcttacatat ccgggcattc taccttaccc aacgcttccc
     3481 aataattgct ctgattatta cgttccatcg ccacgatggc ctcgcgtata tgttgtttat
     3541 tgttttcatc tgacaacatt aacttttccc acgcttcatc tgataatgac gcctgtggcg
     3601 tgcaaatttt acgtaaatct tgtaataccg tggttttctc ttttgcggac tgcagtgagc
     3661 taaaaggcga ctcggcgtat acgatgcctg gcatagcgaa aaaagtgaaa ataagcagta
     3721 gtgtcgttgg tttcatggat tcaacctttt acaaatgtgt atcgaatgaa gcataagtgt
     3781 agtttgcttt catcgctctc atcatgctgt acgaaacgcg cttgcgtggt cagtaagaag
     3841 tgccagactt tatattccac ttttattcct ttttattctt atcgatagcg tttcgttttt
     3901 taaaccgcag cgaccttacc gctatagtca ggtaatcatt aataaaagga taaaaaaatg
     3961 aaactgacaa cacatcatct acggacaggg gccgcattat tgctggccgg aattctgctg
     4021 gcaggttgcg accagagtag cagcgatgca aaacacatta aagttggcgt tataaatggc
     4081 gcagaacaag atgtcgcgga agtcgctaaa aaggtggcaa aagagaaata tggcctcgat
     4141 gttgaactgg taggatttag cggttcgttg ctaccgaacg atgcgacaaa tcatggcgaa
     4201 cttgatgcga atgtcttcca gcatcgaccc tttcttgaac aagataatca ggcgcatggt
     4261 tataaactgg tagcggtggg aaatacgttt gtcttcccta tggcgggtta ttccaaaaaa
     4321 atcaaaaccg tggcgcaaat aaaagaaggc gcgacagtgg cgatccccaa cgacccgacc
     4381 aaccttggtc gcgcactttt actgttgcaa aaagagaagc taattactct gaaagaggga
     4441 aaagggttat tacctaccgc gctggatata accgataatc ctcgtcattt gcagattatg
     4501 gaactcgagg gggcgcagct cccgcgcgta ctggatgatc ccaaagttga tgtagcgatt
     4561 atcagcacca cttacattca gcagacaggg ctttctccgg tgcacgacag cgtatttatt
     4621 gaagataaga attcgccgta tgtgaatatt ttggtggcac gggaagataa taagaatgca
     4681 gaaaacgtga aggaatttct gcaatcttat caatcacccg aagtcgctaa agcggcagaa
     4741 accattttta acggtggcgc ggtgcctggc tggtaattct cactcatgcc gcgccggacg
     4801 atttattttt ctggcgcggc gacgggaatc cattgaaatc aaaatcaccg atgacagaat
     4861 cagcagcgtt cccagccagt ccggtaatgt gaacgtgatc cccagcaaca gcaaagagag
     4921 tagcgcgcta cttaatggtt ctgcacagct caaaatgctg gcttttggac cgccaattaa
     4981 ttgtgctcct ttcaggtaca gactaaatgt cagggacgta ccaatgacca ccaaataaaa
     5041 aaacgccaga atcaaactgc cgttaacgac aaagtttgtt ccttgtctgg cataaaaagg
     5101 caacagaatc agaccgccaa tcagcatact ccagccgacg actggtaacg tgccatagcg
     5161 ggcaattagc gttgagggat aggtggtata gaatgcagca gcaaatgccg aggcaatgcc
     5221 ccagaacaac gcggcaggag agatcgataa tgacgtcgga ttaccgtgtg tcaccaataa
     5281 aaaagtcccg accagcgatg tcaaaatagc gcagaaaacc agaatgcccg ggcgcgattt
     5341 acgcaccagt gagaaccagg cgacgataat cgtcggtgag aggaattgca gcaccgttgc
     5401 cgtggctgcg ttcgattttt cgatggttag caaaaaagtg agctgtacag ttagcgcgcc
     5461 aaccacggaa aaaatcagca ggctaatggc atctttatga ttgttaataa tagaaaagat
     5521 tttatcgcca tgaacaaatg acagcgtcag tagaatcaaa ccggcgaata tcaaacgcgt
     5581 catagtcaaa aactgcgacg acatctggct ttgctccatg atgtattgcg cgcagacccc
     5641 tgaacttccc cacaacacgg cggcaatcag aacgttcagc atcccctttc tggtggaacc
     5701 catttttccc tctgcatact gttttgtcgt ttatgtatgc aggcatgata gcaaaaatgg
     5761 cgaggatggc tctgtttgcg ccacgccgat ttttcttagc ccagactggt gagctaaata
     5821 tctttaataa acagcaggca aatcatcgca atacccaaca tccctatcgc cagccagaac
     5881 aacgcatggt agctccagat ttcaaccata attccgtcaa cagagccggc gataatccag
     5941 ccgacgcgtg aagtatttgc atataacgtt gtcgcagagc ctattttttc aggcatcaag
     6001 tcctgaaaat aaagcatgcc gataccacag agaataccaa ggaagatggc attaagaatt
     6061 tgcaattcca gctcaaccgc cggagtcgtc gccatgagta cgctggcgta aaaacacatt
     6121 ccactcacga tagcaatgag cattaatagt cgcttgccaa tacgtttcat gtaatagcct
     6181 gcgattaaca tcatcggaat ttccagaccg gcagcgatac caatcatttc tccagccagt
     6241 ttatcggtta gatgcagttc atcaataata aatagcggca tatttatcat gtagagatta
     6301 ttcgccgccc acatcattga acagaccaca aaaagtagcc gcgtatcccg cttcctgtgg
     6361 gtggagggta aaatttctac gggttgggta acgacaggaa tatttctttg tattgatggc
     6421 aaaaacaacc agactatcag tccgcaaaca acaaatgcga tggcagcggt gagatacatc
     6481 actttaaaac taaatcccat tgccagttca taagcgagcg gtggcccgat aacccaggca
     6541 agcgagatct gagcacgtaa aaatgtactg aacatgaccg tctcacggcc tgttctgtcg
     6601 gcgtgttcac gggcgagggc gaacatttgc gggtttgcgg tggaagcaaa actactcaga
     6661 agtacgcccg ttgagaggag aatgaagtag ttgcgattcc acgcaaaaag cgtgcaggcc
     6721 agcactccaa ataagcaaca tagcagaatc agtaatttac ggtcgccttg tttatcggag
     6781 tgccttgcca gaaattgact gaccagaatt cccataatag cgctaccggt gaagaaaaaa
     6841 cctaccatta taggacgggc tttcagttca tctgcgagga atatacttag ggtaggagtc
     6901 tgaagagcgc ccgcaatacc cgtcagaaag gcgacaagta aaaatgccgc ggcagtgaga
     6961 tcaagtattt ttgatggagt ggtagccgtt ttttgcattc ttctgaatca ccgggcgaaa
     7021 aaatataact gagttacaaa taagtaccag tcgaatgagt attgtattaa gaatttttga
     7081 gtatcgctga tacaaccaga aa
//
LOCUS       DAZFFI010000180         6948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFFI010000180 DAZFFI010000000
VERSION     DAZFFI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.182"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(116..1765)
                     /gene="pgi"
                     /locus_tag="ACHJ12_004669"
     CDS             complement(116..1765)
                     /gene="pgi"
                     /locus_tag="ACHJ12_004669"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4992347.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2002..2198
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2290..3639
                     /gene="lysC"
                     /locus_tag="ACHJ12_004670"
     CDS             2290..3639
                     /gene="lysC"
                     /locus_tag="ACHJ12_004670"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4992348.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3784..4710
                     /locus_tag="ACHJ12_004671"
     CDS             3784..4710
                     /locus_tag="ACHJ12_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO4992349.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4746..5537)
                     /locus_tag="ACHJ12_004672"
     CDS             complement(4746..5537)
                     /locus_tag="ACHJ12_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO4992350.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5543..6670)
                     /locus_tag="ACHJ12_004673"
     CDS             complement(5543..6670)
                     /locus_tag="ACHJ12_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO4992351.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1717 a         1809 c         1658 g         1764 t
ORIGIN      
        1 aaaatctatt ttaggattaa cctcacggta tgatttccgt taaatcacag acaaggggat
       61 ttatctgata aaacaatgcc gacataacgt cggcattgtt attaaggttt ttaaattaac
      121 cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg ctgctgattt
      181 ctttatcatc tttcagctct ggcagaatac ggtttgccag ttgtttaccc agctccacgc
      241 cccactggtc gaaggtgaag atgttcagga tcacgccctg agtaaagatt ttgtgctcat
      301 acagcgcaat caacgcaccc aggctgaacg gagtgatttc acgcagcagg atggagttgg
      361 tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt gccggatctt
      421 taccctgatc acgatattcc tgctcaacca cttcgcggga tttaccaaac gccagcgctt
      481 cggtctgggc gaagaagtta gacagcagtt tctggtgatg atcagagagc gggttatggg
      541 tgatagccgg agcgatgaaa tcgcacggta ccattttggt tccctggtgg atcagctggt
      601 agaacgcgtg ctgaccgtta gtgcctggtt caccccagat aatcgggcca gtctggtaat
      661 ccacaacgtt accgttacgg tcaacatact taccgttgga ctccatattg ccctgctgga
      721 agtacgccgc gaaacggtgc atatactggt catacggcag aatcgcctca gtttccgcac
      781 caaagaaatt gttgtaccag atgccaatca gcgccagcag tacaggcagg tttttctcgg
      841 caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc agaaagcagt tcaacgaagt
      901 tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa gagtaacggc
      961 cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac tcgccaacgg
     1021 ctttggcatt ggttgagagc gccgcaaagt gtttcgcaac gtgtttttca tcacctgccg
     1081 ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat ggtttcctga gtggtgaagg
     1141 ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc acttccgcga
     1201 tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtgatttttg tacggacgca
     1261 gtgcttcggt caccatgtac gggccgaggt cagaaccgcc gatcccgatg ttcactacgt
     1321 cagtgattgc tttgccggta taacccttcc actcaccgga gataatcgct tccgagaagg
     1381 ttttcatctt ctccagcacc gcgttgactt ccggcattac gtctttgcca tcaaccaaaa
     1441 tcggggtatt gctacggtta cgcagcgcta cgtgcagcac agcgcggttt tcagtgcggt
     1501 tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac tctttcgcca
     1561 gatcttgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa tccaccagca
     1621 tctgatcgcc gaaggttgcg gagaacttag aaaaacgatc gccgtcttta gcaaaaagat
     1681 cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc caggcagcgg
     1741 tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga attgtgactt
     1801 tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt tagcgtaatg
     1861 ttgagtgtaa acccttagcg cagtgaagca tttattagct gaactactga ccgccaggag
     1921 tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc ccgctcaccc tttatttata
     1981 aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta acggtgttgg
     2041 aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt gaacggctgg
     2101 ccacgttcat catcggctac aggggctgaa tcccctgggt tgtcaccaga agcgttcgca
     2161 gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga agtatcgctc
     2221 tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat cccctgacac
     2281 gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt agctgatttt
     2341 gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtgcg tttagtagtc
     2401 ctctcggctt ctgctggtat cactaatctg ctggtcgctt tagcggaagg gctggaacct
     2461 ggcgagcgat tcgaaaaact cgacgctatt cgcaacatcc agtttgccat tctggaacgt
     2521 ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tgctggagaa cattactgtt
     2581 ctggcagaag cggcggcgct ggcaacgtct ccggcgctga cagatgagct ggtcagccac
     2641 ggcgagctga tgtcgaccct gctgtttgtc gaaatcctgc gcgaacgcga tgttcaggca
     2701 cagtggtttg atgtacgtaa agtgatgcgt accaacgacc gatttggtcg tgcagagcca
     2761 gatgtagccg cgctggcgga actggccgcg ctgcagctgc tcccacgcct caatgacggc
     2821 ttagtgatca cccagggatt tatcggtagc gaaaataaag gtcgtacaac gacgcttggc
     2881 cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc atctcgtgtt
     2941 gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtagt ttccgcagca
     3001 aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt tggtgcaaaa
     3061 gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccggt ctttgtcggc
     3121 tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga aaatccgccg
     3181 ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc tcactttgca cagcctgaat
     3241 atgctgcatt ctcgcggctt cctcgcggaa gttttcggca tcctcgcgcg gcataatatt
     3301 tcggtagact taatcaccac gtcagaagtg agcgtggcat taacccttga taccaccggt
     3361 tcaacctcca ctggcgatac gttgctgacg caatctctgc tgatggagct ttccgcactg
     3421 tgccgggtgg aggtggaaga aggtctggcg ctggtcgcgt tgattggcaa tgacctgtca
     3481 aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa cattcgcatg
     3541 atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga agatgccgag
     3601 caggtggtgc agaagctgca ttttaatttg tttgagtaat atttccctac tggcgtataa
     3661 cgctcaccga ttgttatacg ccatcctcac tgaaaacata gattaaataa tatctgaaaa
     3721 gcaaaatcac tcctgtagaa cagtattata atcaccgaaa aatatcattt aacaggagtg
     3781 ttaatggcta ctttaatgga aaaagatgca cttttaaacg gagccagtca gtgcatcgcg
     3841 tttttatcaa atatcattga taattgctca gtttcgtcgc atcaggattc aggcgatgct
     3901 ctcaagcgat tagtcagcta tcgcgattat ctttattcta cccccgctga acttgttgat
     3961 tttacacaag gtaagattct ccttcagcag gtaagaactc aatatcagca tgaattcaac
     4021 aatactacgc attcagaaaa taaagcatct tttgattcta tttggcagcg tctgacaaat
     4081 catgaagtca caccgcaaca gcatcccatt ggttttgttt taggtgggca acccggagca
     4141 ggtaaatcat ctctgattga attagccaaa agagagacaa aagataatat aatgattatt
     4201 aacggtgatg attttcgatt tctacaccct gattttaatc atatctacca aaattatggg
     4261 gatgattttg ttacccatac agcgaaattt tccggcgaaa cggtcgaacg tgcaattgag
     4321 agagcgatag tcagtaaact caacattgtt gtcgaaggaa catttcgtaa tgccgctacg
     4381 ccgttacaaa cattaaaaaa actcaaagat gcaggctacc aaacagaagt catgataaaa
     4441 acaacctcag cggcattaag ctgggaaagc accaatgaac ggtataataa ggacaaagag
     4501 gcgggaaata ttgcccgaaa agtggataaa aaccaccatg atatcgtcac cggtttgctg
     4561 gcagaaaatg cccgcaaggt gtttgcttca aacctggcag ataagtttgc ggtgtattcg
     4621 cgtgagaaga tgatatttag ttcacaagcg gcaacgaatg acgatattgc aacgcttata
     4681 cagaatgaaa tcagcgggaa tacacaataa taataagagc tgatggcgtg aaataacgcc
     4741 gggcatcacg cccggcgcat taccgtctgg tacattaagc tatccagttc cttaacgtcc
     4801 ccttctctgc tatccggtaa cagcggtgga atttccccac gcgccagttc cgcacgaata
     4861 aacgcatgta acaatggacg gcgcggacca tattccgctt ctcctgcgcg ctgtttacgt
     4921 tccagtaatt cgtctatttc tgcacgtaac ggcgcatcaa gttcgcttcc cgccagtagc
     4981 tcagcaaagc gcattggcgg tacgcctttt cccgcttcaa cccaacgcac ggccagcagc
     5041 ggacgcagca cgtaaaagta ttttttcagc cgcacttcgt cgccttgtaa ataaccgcga
     5101 aagtttttcc gcgccatcga ataataatgc caacgcgcac gcagcggaga aaaccacttc
     5161 ggcaccattg ccttaagcgc cgttatcgtt tcttcatctt gttgatagac cacaggagaa
     5221 tccagccatt ctatcagcgt cggattcgca cctttcaata atcccagcgc cttgcgccac
     5281 tcccaaccgg aaacatctaa ctcgtcgtcg atgggcagct caataacatc gcgcggtgac
     5341 tctactcgca gataccattc cagcgggtga acatacaaaa accgtacatc gtagtcgctg
     5401 tcgggcgagg caaatcccca gccgcggctc cctgattcac aggcgtacag tactttcaca
     5461 tcataccgcc gttcaatttc ttttaactgc tgcgataccc tttcgcgcat cgcagcactg
     5521 actccattta atggcatgtt actcaccctt tgatattaac caacccatgc agaacctgat
     5581 ccagaccgcc aaatacagag atgttatcga tccgctcagc cactctctcc agcgtttcta
     5641 gctcttttaa acgcaacgct accggattgt tctccatgac tttggcggtg ttcagtaacg
     5701 agcgcgtcgc tgccgtttct tcacgtcggc gaatcacatt cgcctgcgca gatttctctg
     5761 cctcaaccag ctgcgcgagg atatttttca tatcaccagg caggacgata tctttcaccc
     5821 ctaatgaggc aatctccata ccaaatggca acatgcgcga tttcacctgt tcgctgacaa
     5881 cgtcatcaat gacctgttta tcttccagca gttcatccag cgtacgagta cctaccgctt
     5941 cacgcagggc aaattgcaat tcccgataca aatgatcaat cggcttcgtt aactgactaa
     6001 atgccagcaa cacgtcgctg tagcgccagt tagcggcgag gttaatccgc agattgacct
     6061 tatctttcgt taagatttct tgcccgctga cttccagtac ctgcaagcgg gtgtcaacca
     6121 cttcagcgtc aaccagatgg ttaattttcc agtatgcggt cagccccggc ggtaacagcg
     6181 cctgcgtttc accatcgatt ttcagcacgc ctacatgcca ggccggaacc tgcaccgtta
     6241 gtattgcatc gcggccttta actgcccctt tgcgccgtgg ttgcaatacc gcattcatca
     6301 cctccgtttg tacctggact tgctgcgtat tcatccgcac cagtgtcaaa tcatcctcta
     6361 cacgccagta caaacggcgc gttgatggcg gcaggatctc ctgcaaaata ccgtccatat
     6421 acaacgcccc ggcttcaatc tccgacaagt ctgccaccag gcaatatttc tccacccaat
     6481 caggctgaaa gcggcgcaaa taatccgcca gcacgtcggg aacttcgctg ccatcgagcg
     6541 taatcagcaa gacttctggc gtatttaacc agggtaaaag atgctcgcct gcatgtagca
     6601 ctttgtagta atcgccgttt ctggagagta atgccagctg atcttttctt acgcttattt
     6661 ttttaatcat tatctattcc ttgcttaaca gccgtcgcgg aagcagacac gaagcgacag
     6721 gggaattccc ttttgcctgt tacccggcat ctttatcggc gacgttatcg gggatcgctg
     6781 actgcgcaat ccctcacttt ttatttaata agttaaaacc agtattcatt tcaggaatga
     6841 atgtgggaat cgaacccata attggcgtca aaggctgacg gaacacgccc ggaaaacagc
     6901 ctggccgttc ttggtgtgcc gacggggttt gacccccttt gtaattca
//
LOCUS       DAZFFI010000181         6879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFFI010000181 DAZFFI010000000
VERSION     DAZFFI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.183"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            332..601
                     /locus_tag="ACHJ12_004674"
                     /pseudo
     CDS             332..601
                     /locus_tag="ACHJ12_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(832..930)
                     /locus_tag="ACHJ12_004675"
                     /pseudo
     CDS             complement(832..930)
                     /locus_tag="ACHJ12_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      1051..1239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1304..3148
                     /gene="btuB"
                     /locus_tag="ACHJ12_004676"
     CDS             1304..3148
                     /gene="btuB"
                     /locus_tag="ACHJ12_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4992352.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            3162..3353
                     /locus_tag="ACHJ12_004677"
                     /pseudo
     CDS             3162..3353
                     /locus_tag="ACHJ12_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3494..5581)
                     /gene="iha"
                     /locus_tag="ACHJ12_004678"
     CDS             complement(3494..5581)
                     /gene="iha"
                     /locus_tag="ACHJ12_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4992353.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            5520..5702
                     /locus_tag="ACHJ12_004679"
     CDS             5520..5702
                     /locus_tag="ACHJ12_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992354.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHGNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            6122..6315
                     /locus_tag="ACHJ12_004680"
                     /pseudo
     CDS             6122..6315
                     /locus_tag="ACHJ12_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            6413..>6879
                     /locus_tag="ACHJ12_004681"
     CDS             6413..>6879
                     /locus_tag="ACHJ12_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4992355.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKD"
BASE COUNT         1833 a         1596 c         1591 g         1859 t
ORIGIN      
        1 gcgcagttcc ggatgtatgc tgctaacgca tccattccta cttcaggtat ttggcgtaga
       61 agcggatgct aaaaacttgc cgcaaaaccg caatatcagc ctgcagtatc gctctccaga
      121 ttatccattc ctatattgat gacaacgaca ggtaagaccg tgacctgata aacctgaata
      181 agctcctgga cataaagtcc gtaatgggaa taaggcgaga gtcagcgaat ctcatcgagg
      241 ccagcagcac tggctgcaat aaggatatag agcagcaatc taccgtcaac gtcaaaaaca
      301 cggaagcctt gcaaacggga tgcgttcata taaacaacat ggagcagcaa caatacgatg
      361 cctgtcgttg ctcagtcagg atcttttctg tcatgcgcga gccagcgtca cgattgataa
      421 ataatgttag caactgatta atttactgca acagacgtgg ttggtaataa gcactggcgg
      481 acaatgcagg atggaaggct gttttcaggt tgtccgttca ggtacgttag atcagttcgt
      541 tgcgctaaag acaactgttc agtcccttat gttacccgtt atggcaacca ttgcccggtg
      601 ataaccgggc gtgtcatctg tgcatccgtt gattcgtgtg gactgataaa gttttgtctg
      661 ctgaatcttt atacggaatg ctcttcagta tgcgtaagat aatttgtttt ttggaggatt
      721 gtcgacaatc agagggtaaa caaaagaatg gttgaatgtg gatgaggttc atattattga
      781 ttcagatttt ttgaagaatc accacatggg atgtccatgt ggttgtataa ctcagggcca
      841 gcgcttaata atgatggatg tattaatgcc gccaaaggca aagttgttac tctgcaaaaa
      901 cacacaatca accttgcggg cttcatgcaa ctaattgagc atatatgttt tattcttatt
      961 atggtcgccg ataaaatatt atgtgtatta gatccgagtg ttcatgcatt cctatttttg
     1021 agtcatgtat ggacagtaac ttgttacaac cagtaatatc cagttgtcgg tcctgcgagt
     1081 taatagggaa tccagtgcaa atccgaaact gacacgcagc ggtaaggaaa ggtgcgatga
     1141 ttgcgtaatg tggacacggt catccggtgg gtggtcatct ctttgtatct tcagatgccc
     1201 ttctaggtct gaagacctgt cggttaacgt cgcatctggt tctcatcatc gcttactatc
     1261 gatggtaact gcgatattct tcttctgttg tggatgcgtt acaatgatta aaaaatattg
     1321 gtttctaatg gtgtactccg tcacagcctt ttctgtctgg gcacaggata cccctctgga
     1381 tactctcgtt gttactgcta accgttttca ggaaccatta agtaccgtgc ttgctcctgt
     1441 aactattgtg acacgagaag atatagatcg ctggcaagtt tcttctgtaa atgatgttct
     1501 gcgtcgtttg cctggcgttg caatttctca gcatggggga gaagggcagc tctcaacaat
     1561 ttttgttcgt gggacaaact ctaaccacac tctggtattg attgatggag tgcgcctgaa
     1621 tttagcgggt gtcagtggag ctgcagacct gagtcagttt cccgtcgctt tagtgcagcg
     1681 tatagaatat atccgtgggc cacgttcagc aatttatggt tcagatgcca ttggcggagt
     1741 gataaatatt attacatccc gtgagaatac tggcactgaa atttccgcag gatggggaag
     1801 taatagttat caacattacg atatatccac tcatcagcaa cttggggaaa atacccgtgt
     1861 aacacttctt ggcgattata cctatactag ggggtttgat gctgttgcct atggcagtac
     1921 agggatgcaa ccacagtcag acagagacgg ttttttaagt aaaacattct atggcaaact
     1981 tgaacataac ttatctgata cgtggagcgg atttgttcgt ggctacggct ataataaccg
     2041 aactaaatat gacgcctggt actctcccgg ttctccactg attgatactc gaaaacttta
     2101 tagtcaaagc tgggatgcag gtcttcgcta cgctggtgaa acactgcaat ctcaacttgt
     2161 ttccagttat agccatagta aagattacaa ctacgatcca cattatggac gctatgacac
     2221 atcagctaac ctggatgata tgaaacaata caatctccag tggacaaata gcgtcacagt
     2281 cggccacggt aatgttggcg ctggtatcga ctggcaaaaa caatccacta ctcctggaac
     2341 tggatatctg ccaaaaggtt acgatcagcg taacacgggg atttatctga caggactgca
     2401 aaaacttggc gattttacgt tagaaggcgc ggtgcgtaat gacgacaact cgcaatttga
     2461 acgccacaca acatggcaat ccagtgcggg atgggagttt attgagggtt atcgctttat
     2521 cgcttcctat ggaactgctt tcaaagcgcc aaatctgggg cagctatatg gtatgtatgg
     2581 taatccgaat ctggctccgg aaaagagtaa acagtgggaa ggggcatttg aaggtttaac
     2641 tgggggcgta aattggcgta tttcaggata ccgtaatgat atcagtgaaa tgattaacta
     2701 taatcctcat acccttcgtt attacaacga cggaaaagta cacgttaaag gaattgaggc
     2761 tacagtaaac tttgatacag gagcactgac gcatactgtt agttacgatt atacggatgc
     2821 cagaaatgcc ttaactgaca aaccattgga acgtcgtcca aaactgcaag tgaagtatca
     2881 acttgactgg caagtattcg attttgactg ggggataact tatcaatata tgggatcgcg
     2941 atacgattcg gactactcat cctggcccta taagtcagtc aaaatgggcg gtgtgagttt
     3001 atgggatgtt gcggttgcgt atccggtcac tcctcacctg atagttcgcg gtaaaatagc
     3061 taatctgttc aacaaagatt atgagactgg ttatggctat caggcagcag gacgggaata
     3121 tatattgtct ggcagctata ctttctgaaa tctgccccac cgagtcgcta agcagcgtga
     3181 tccgccatgc gatcaaaaag cgtaaagtgt tcccgacaga cgaggcggtg aaaaaagttg
     3241 tgtggctggc aatccagtct gcgtcccaga aatggacgat gccgttgaag gactggcgta
     3301 tggaaatgag ccgctttatt atcaagttca gtgaccgcct ggacggtcac ttctgggaaa
     3361 aggcatttac acagaatcct gaacaggctc ggtcacttcg accagacacc gtgcgtcatg
     3421 taacgttgcc actgttctgc caggagttac agtggcaacg tatgctaccg tcagtgatgg
     3481 cgttttgttg ttatcagaac tgatagttca gcgacatcca gtaatttcgc tcaggtatca
     3541 catatcccgt tgttgatgat cccgtctgga agtaatcacc ggcataaagc gtactcttac
     3601 cggcactgta caggctcacg tcactgtaat ccttgttgag caggttattc accgcagcat
     3661 tcagcgtcag ggcatccgtc accttccacg acagacctgc atccaccacc gtccaggctt
     3721 tcagatattc tcctttctca tcatacactt tcttctgtac agcgctcagc gacgaataat
     3781 tccgggtgaa acgtggtgtt ttcccgcgat aacgggcacc cagccatgat gccacctctt
     3841 cggtgatctg ccagttcagt ttcgcattca ccatgtgttc aggggtataa ctcagcggtg
     3901 cacctttgtt atcaccatca cgttgttcac tttgggtcca ggtgtaattc agtgacagag
     3961 tgacatcctc tgaccacagc ggcaatgtgc cggcgaattc cacaccatgc aagcgggcct
     4021 ttccgctgtt tgtatagcta ttggtgttat catcaatgga ataagagaca atcttgttgg
     4081 agaagtctgt tataaagcct gttatattgg cattcagacc ggagaggtta tcgtaatata
     4141 cccccgcctc ataacttaca ctctcttccg gcttcagttc ggggttaccg agtagattag
     4201 tacgtccctg cccggataca ccgctgatcc ccttatgtaa ctgtcccatg gtgggggctt
     4261 tatatcccgt ggtcacaccg cctttcagcg tccaggcatc ggtcacgtcc cagaccagat
     4321 atgcacgcgg actgaagtgt cccccgaatt gatcatgatg ttcatagcga ctgcctgcag
     4381 tcagcgcaag tacatccgtg agatgccact catcctcagc aaatactgac cagcttttct
     4441 gtcggaaagt ttcacctgtg ctggcaagga caaccccatc tttcatggac gaactctgat
     4501 actctccccc cacagtaagc agatgagatt ctcccagagg ggtaagcagc aatgaattca
     4561 ggataaggtt agattcctta agctcccgcg gtttaccggc aagcccccat ttatcgctct
     4621 tcagaacact acgtacaagc tcacgacctt tattttccgt ctcgttccag ttcagatacg
     4681 atttccaggt accgaaggtg aaagtatgat catagccagc cgaaattttg tttcgctcat
     4741 agcgcagagt atggtcatat ccccctgtca ggctacccag ttgaccatcc tggttatcgt
     4801 aacgctgtcg ggtggtatcc atatcaaacc agagcacatc ctgctccgta gccttccagt
     4861 caatacgcgc accaagatta taattctgtg actccgtggg ataaggaata cgcgtggctg
     4921 atgtgtcgct cagtgatgtg accgatgagc cctgacgctg ctgtgtgctg ccgcggatct
     4981 gcagactgac agaatcatcc acaagagggc cactgctcca gaaattaaac tggctgctgt
     5041 tgccccattt gttactctcc tgcagattca gcccggcgtt gacggaagag agccatttgt
     5101 ctgcattctt tttggtaatg atattcacca caccacccat cgcatcagag ccatacagtg
     5161 tggacatcgg ccccctgata acctcaatac gctcaatcgc ggccagagga ggcatgaacc
     5221 ctgtattcat ggcagaaaaa ccgttaggcg tcacgtcccc gctcccgccc tgacggacac
     5281 catcaatcag tatcagcgtg taactggcgg gcattccacg gatgctgatt tccagcccac
     5341 cggttttacc cgtaccactt tcaacatcca caccctccac agctctcagt gcctccgcca
     5401 gatcgtggta ctggctggac tgcaattcct cctgactgat tacggaaaca ctggcggctg
     5461 cgttggtcag ttttttctca tagccggatg ccgagacaat catcacatcc tctgctgcca
     5521 tgctgctggc tgaaaacccg agacagggaa tgacaacgga agccagagtg gttattcgca
     5581 tattactaat ctccgatcat ggtaatcggc aagacaatac acaaatacaa acaataatga
     5641 gaataattta tatttacctt ttacaaaaaa tgaatattac tgacaaaact aaatataaat
     5701 aatgactgcc tcggcatgcc agttaacagc catccgcaat ggcgtgttcg attttgcgaa
     5761 taaaaaatgg ttactgtaga agcggatttt caccacctgt caaccatgaa tggtcagtgg
     5821 agtgagacga ctggtatgat ttggtcgatg acttcttccg gtgaatacgg acagaaacat
     5881 tgaaatattg taagagagaa cgttcagaat tctgtgtcac gtgaaaatac tacagcgtta
     5941 tcaccttatt cagtcgacct tcaaaataaa cgtctaaccg tgacaatcac catttggatt
     6001 gcccactgga gaggcgaacg attgcctcga cggcatttat aatgccagcc cacattgttg
     6061 gcgctgacaa tactaaggac aaacgatgag ggagaaccgt cttataccac tagcgctgac
     6121 accggcacac cttccccgtg aaatacaccg gctggagcct gaagaaagct gttgcccgga
     6181 gtgtggcggt gagctggatt atctggggaa gtcagcgctg aacaactgga actggtgagc
     6241 agctccctga aagtgatcag cacagaacgg gtaaaaaaag cctgtaaaaa atgtgacggt
     6301 accgttgaag cgccggtctc gtaagaataa tcctgggcaa ataagatttt tcctgaaaaa
     6361 gcgtctagtc ttttgatatc gttgttttta tgctaatcag ggagtatatg tgatgattgt
     6421 ctgttacgtt attcttgtta accgtccgtt tgaaacagag attgccggga aaaagctatt
     6481 ttttcagaaa aatgacgtcc tgttgacaga taacatcagc cagtcctttt tcctgcgcca
     6541 tcccggtaat gttactgagc tccggattga cgaatgcatt gtcagaaaat atatggaaag
     6601 tgccgacagg atccagcact ctgatagtgc tgtttctgtt ccgttcatta aaacaacctt
     6661 ctctcacatc gctctgttac ataatgtcat gcagaatctc agtctgcaaa agacattgcc
     6721 acaacaattt tctgagcaga tgttttttgc ctgcctttcc attttttcca cacacagagg
     6781 tttcatgttt ctgctattca gatgcataaa ctctgttgcg cttaaagtca ggtatattat
     6841 ctgttccagt ccatcacggg catggagagt taaagatat
//
LOCUS       DAZFFI010000182         6860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFFI010000182 DAZFFI010000000
VERSION     DAZFFI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.184"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..285)
                     /locus_tag="ACHJ12_004682"
     CDS             complement(<1..285)
                     /locus_tag="ACHJ12_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO4992356.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMA"
     gene            complement(282..1952)
                     /gene="menD"
                     /locus_tag="ACHJ12_004683"
     CDS             complement(282..1952)
                     /gene="menD"
                     /locus_tag="ACHJ12_004683"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4992357.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2041..3336)
                     /gene="menF"
                     /locus_tag="ACHJ12_004684"
     CDS             complement(2041..3336)
                     /gene="menF"
                     /locus_tag="ACHJ12_004684"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4992358.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGETAFLGSSPERLWRRRDNALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3415..3720)
                     /gene="elaB"
                     /locus_tag="ACHJ12_004685"
     CDS             complement(3415..3720)
                     /gene="elaB"
                     /locus_tag="ACHJ12_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4992359.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3775..4236)
                     /locus_tag="ACHJ12_004686"
     CDS             complement(3775..4236)
                     /locus_tag="ACHJ12_004686"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4992360.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4301..5218
                     /gene="rbn"
                     /locus_tag="ACHJ12_004687"
     CDS             4301..5218
                     /gene="rbn"
                     /locus_tag="ACHJ12_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4992361.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5406..6626
                     /gene="elaD"
                     /locus_tag="ACHJ12_004688"
     CDS             5406..6626
                     /gene="elaD"
                     /locus_tag="ACHJ12_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO4992362.1"
                     /translation="MMVTVVSNYCQLSQKQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDKFGKEAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     FPLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSEFMGEMSNNNIKVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
BASE COUNT         1775 a         1795 c         1600 g         1690 t
ORIGIN      
        1 cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt
       61 gtaactaacc aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat
      121 cgccgccgaa ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc
      181 ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
      241 gggtaaacct ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
      301 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc
      361 gtggtggttg gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc
      421 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
      481 atcagataga aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg
      541 ccgttgttgt tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc
      601 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
      661 gcccgctgaa cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg
      721 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg
      781 ctgttaccaa caaacaattg cccctgttca ggcagatagt cgcagatgcg atgcgccagt
      841 tgcgcttcgc caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg
      901 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
      961 gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg
     1021 tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc
     1081 cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct
     1141 ttggcattgc ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca
     1201 tcgccaatca gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc
     1261 gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc
     1321 tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
     1381 tcacccagac gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca
     1441 gcaaacgggc agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg
     1501 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc
     1561 gtggggtgag aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt
     1621 agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca
     1681 atcagtgccg gatagagatt tgctaccgcc gtgccggagg tcacaatcac cgccaccggc
     1741 tgcttgctga ctttcgccag ccccagcgcc agatgtccca gtccacgctc atcgaaatgg
     1801 gtgtggtgaa tgaatgtgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
     1861 ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
     1921 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
     1981 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
     2041 ctattccatc tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc
     2101 ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc
     2161 gctaattttt gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc
     2221 cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc
     2281 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
     2341 atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag
     2401 acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca
     2461 gatatcttcc accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc
     2521 acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg ccagcgcttc
     2581 agtacgcagc gcgttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc
     2641 agtttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc
     2701 agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag
     2761 caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt
     2821 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa
     2881 gggcttgata ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag
     2941 ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc
     3001 caggcggggt aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg
     3061 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
     3121 acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
     3181 ttgcgggtac gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
     3241 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
     3301 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
     3361 tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
     3421 gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca
     3481 gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata
     3541 actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
     3601 tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag
     3661 tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat
     3721 tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc
     3781 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt
     3841 cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta
     3901 aacaggctta tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagttgctg
     3961 ccccactttt tcgccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc
     4021 aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat ttttccaacc
     4081 aagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt
     4141 ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga
     4201 taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc
     4261 cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg
     4321 tacttcggcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca
     4381 acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct
     4441 actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg
     4501 cgatcatctt tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
     4561 acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat
     4621 tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct
     4681 cgatgatggc ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg
     4741 ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc
     4801 tggcgtgccg cctggtccac tgtttcagga attaaaagcg ggcaaaacaa tcaccctgga
     4861 agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct
     4921 cgctattttc ggcgataccg gcccctgcga tgccgcactt gatctggcta aaggtgtcga
     4981 tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg
     5041 ccatagctct acacgccagg ctgcgacact ggcccgtgag gctggagtcg gtatgctaat
     5101 cattacccac gtcagctcgc gctatgatga caaaggttgt cagcacctgt tacgtgaatg
     5161 caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta acgtttaact
     5221 ctgcttatta ttaaaaacag ggcgaaactt gccctgttat cgcaacccgc gctacatata
     5281 tccacccaca cgggcatatt gagcgatttt tgatatttat tcatctcctc acgcactata
     5341 ttcagagcaa taagttgcct ctatttatct tgattcagca caacctttgc aactaaaagg
     5401 agcccatgat ggttacagtt gtcagcaatt attgtcaatt atctcaaaag caactcagtc
     5461 agacatttgc agaaaaattt actgtgaccg aggaattact gcagtcttta aaaaaaacag
     5521 cgttatccgg agatgaagaa agcattgagt tactgcataa tattgcgtta ggttatgata
     5581 aatttggaaa agaagctgaa gacattcttt accatattgt tagaaaccca acaaatgaga
     5641 ccctatcgat tatccgactt atcaaaaatg cttgtttaaa attatataat ttggcacata
     5701 ccgcaaccaa cttccccctt aaaccaactg gtccggataa ttcagatgtt ctcctgttca
     5761 aaaagctatt ctccccttcg aaattaatga caattatcgg tgatgaaatt cctcttatat
     5821 ctgaaaaaca gtcgctttcg aaggtgcttt taaatgatga gaataatgaa ctgagtgatg
     5881 gtacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagat tgctatcttc
     5941 agaagatcgc cgctaatgca aaaaatactg aagtcaatta tcctactggt ctctacgtcc
     6001 cctactccac cagaactcac ctggaagacg ctctcaatga aaatattaag agcgatccat
     6061 catggccgaa agcagtccag ttattcccca taaatactgg cggacactgg atattagttt
     6121 cgctacagaa aatagtaaat gaaaaaaata atacacaaca aataaaatgc gtcatattca
     6181 actcattgcg cgcactaggc catgataaag aaaattcact taagcgtgtc attaatagtt
     6241 ttaattctga attcatggga gaaatgtcga ataataatat aaaagttcat ttaactgaac
     6301 ccgagataat atttttacat gccgatcttc agcaatattt aagccaaagt tgcggtgcat
     6361 ttgtgtgcat ggcagcccag gaagtgattg aacaaaggga aagcaattct gacagcgccc
     6421 cctatacgtt attaaaaaac tatgctgaca gatttaaaaa atattcagca gaagagcagt
     6481 acgaaattga ttttcaacat agactggtaa acagaaattg ttatttagat aaatatggcg
     6541 atgcacgaat caatgcttct tatacacaat tagaaataaa acactcacaa cccaaaaata
     6601 gagcatccgg caaaagagtg agttaacatc attgttaatt aattcgacca gtcagcaaat
     6661 atgctgactg gtcgaacata gtttgctaaa cagtcatcac tgactgatgt cagtcacacc
     6721 atccgccagt tcaatcttcg ggtatttcca gcgtatctcg gcacgcgatt gctggtctgt
     6781 tgatgtagtg gattgttgtt gctgactttt cttaccctca gagttctgcc ccctgtcaac
     6841 tcaatctgcc aataaataac
//
LOCUS       DAZFFI010000183         6834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFFI010000183 DAZFFI010000000
VERSION     DAZFFI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.185"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..251)
                     /locus_tag="ACHJ12_004689"
     CDS             complement(<1..251)
                     /locus_tag="ACHJ12_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HFO4992363.1"
                     /translation="MRSLFALLITVISCTTASAAMTETCQKYFDETDKFLSQMKTTGV
                     AEGQINLMKQQYEQSKKQFSSLPEASQDAACKQASDALK"
     gene            complement(1005..1253)
                     /locus_tag="ACHJ12_004690"
     CDS             complement(1005..1253)
                     /locus_tag="ACHJ12_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4992364.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIH"
     gene            complement(1479..1574)
                     /locus_tag="ACHJ12_004691"
                     /pseudo
     CDS             complement(1479..1574)
                     /locus_tag="ACHJ12_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1571..2137)
                     /gene="yecD"
                     /locus_tag="ACHJ12_004692"
     CDS             complement(1571..2137)
                     /gene="yecD"
                     /locus_tag="ACHJ12_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4992365.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2447..4219
                     /gene="aspS"
                     /locus_tag="ACHJ12_004693"
     CDS             2447..4219
                     /gene="aspS"
                     /locus_tag="ACHJ12_004693"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4992366.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEGDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4337..4789
                     /gene="nudB"
                     /locus_tag="ACHJ12_004694"
     CDS             4337..4789
                     /gene="nudB"
                     /locus_tag="ACHJ12_004694"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4992367.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4818..5558
                     /gene="yebC"
                     /locus_tag="ACHJ12_004695"
     CDS             4818..5558
                     /gene="yebC"
                     /locus_tag="ACHJ12_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4992368.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5593..6114
                     /gene="ruvC"
                     /locus_tag="ACHJ12_004696"
     CDS             5593..6114
                     /gene="ruvC"
                     /locus_tag="ACHJ12_004696"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4992369.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6116..6718)
                     /gene="yebB"
                     /locus_tag="ACHJ12_004697"
     CDS             complement(6116..6718)
                     /gene="yebB"
                     /locus_tag="ACHJ12_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO4992370.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            6789..>6834
                     /gene="yobI"
                     /locus_tag="ACHJ12_004698"
     CDS             6789..>6834
                     /gene="yobI"
                     /locus_tag="ACHJ12_004698"
                     /inference="COORDINATES: protein motif:HMM:NF038179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO4992371.1"
                     /translation="MYIFITHFFTEYVIF"
BASE COUNT         1749 a         1580 c         1755 g         1750 t
ORIGIN      
        1 tgcttaagcg cgtcacttgc ttgtttacag gcagcatcct ggcttgcctc gggcagagat
       61 gagaattgtt tctttgattg ttcatattgt tgtttcatta aattaatctg tccttctgct
      121 actccggttg ttttcatttg cgataagaac ttatctgttt catcgaaata cttctgacat
      181 gtttctgtca tagccgcact ggcagttgtg caggatatta cagtgattaa taaagcaaag
      241 agacttctca tatatcctcc attgtttggt ttgaaaaatt aacgctatta aataactcat
      301 agacaggtaa atttatgtga ttatagtcat aaactataaa aatattgatg ttgtctttgt
      361 agaaaggttg tgagcaatta tgaaaattta tttgcttgtt cactggtttt agggagtttg
      421 atgtgtccat gaagcgtttc tgacgagaaa tcaacataaa tcaccagttg gtgaactagg
      481 attgccagaa acgctcctgt cagttctgta gtgtgtagtg ctcacttaga gcctggtgaa
      541 ctggataatg agtcgcacct cagtatcctg caatgggctg cttttcccct gctcctgatt
      601 cttttttgtt tcaggtctta ccaacataat ggttgttgta ataaattgcg tgtgattctg
      661 tatcgggtca accatgtgac gatacatgtt cagtaaggaa gactgaaggg ttcagagtgc
      721 gagtatgatt aatagatgga atcagcgttt caaagatttt gagggaatat aaggtctgac
      781 ctgatgatta ccttttgcgc ctgtatcctt tgcataaagg aggttgatgt agataaagtc
      841 atctccactt gtgatataaa acagtcagtt atgaaaaatc ggcataaaca tccttttgtt
      901 aaccttaaag ccagacttac ggtttttcca tagttacaga taagcggcta gtaaggtttt
      961 acaggtgata agcctgtaac ggaaatcacc tgcaaatcta ttaattagtg aataaaccag
     1021 tcatccgctg tttcccagac ttcctgaagc gtttcttgta caaactcttg cgcggattct
     1081 ttatccacgg ctcttaaaat agtgagtcca tcattgctgg cagatttaac gataacatct
     1141 acatcgtcat aacgcttacc gacacgtcgt agcatttcct gttgcagggc aggaacagaa
     1201 ccttttggca ttttgctgat tttttcttta gcgatacaga tctcaacacg cataacaccc
     1261 tcctgtaact gtggttatgt gcaggtgtta tttttatctg tatggataac cagtgtcaat
     1321 tcctgatgtt gtttatgggg catcaatggg gcatgtatgg gacacttttt atcggcgaaa
     1381 ttcgtcgaag ttcgtcgaca tggtaaacga atcatctatc caacccttga aaaacggcgc
     1441 tcctggacga tcttcgtcga tttttaaaaa tgttgcgtca cgcagttaaa gtggcgggca
     1501 tactcttcaa ggctggtgat ccccaaccgc acccatttag gatgcgacca ttgcggtaga
     1561 ccgatgtaaa tcataacgcg tgaaggatct cttccacact gcgcacacgg gcgatgcgcg
     1621 ggtagatatg attaatgctg ttattgtgtt gctcggcgct ggcggcacta caggcgtctt
     1681 cggcaatcac cagattaaag ccaagttccc aggcattgcg ggcggtggat tcaacaccga
     1741 tattggtcga gatcccacat aacactattg tatcgatacc tcggcggcgc agttgcaact
     1801 ccagatccgt accgtaaaac gcaccccact gacgtttgat gatttcgata tcactgtcag
     1861 ttgcacctaa tgcagcagga tgttgccacc agttttcggg taacacttta gccggtgagg
     1921 gcgcatcgac cggctgtttt aatgcttcgg cgtaatcggc agaccagcca acgcgcacca
     1981 gaaacacggg ctgaccgctg gcgcgaaatt tcgccgccag cttcccggcg cgattaacta
     2041 cctcatcggc agtatgtgga ccaccggcaa aaggtaagat gccttcttgt aaatcgatca
     2101 ccaccagcgc agtggtttta gcattaagtt caagcataat gactcctgaa aaaacaaaat
     2161 cgtgcctcac accttaatgc gattagccga tggcggaacg acaaattttg ttaattttta
     2221 tgagttcggg caacaccttt atagcaaact ggcgtagagt ctgatttcag gcccgatata
     2281 aagtggtaac gaggcgttcc agcgcgggcc aggctcttac ggtgcggcag aatttccagt
     2341 ataatagccg ccttttttca ttcagttgtg acatacagct aacgctgcga cttggtcacc
     2401 tgcgcagcag ggggcatccg ccggcagcta agttaaggga tatctcatgc gtacagaata
     2461 ttgtggacag ctccgtttgt cccacgtggg gcagcaggtg actctgtgtg gttgggtcaa
     2521 ccgtcgtcgt gatcttggta gcctgatctt catcgatatg cgcgaccgcg aaggtatcgt
     2581 gcaggtattt ttcgatccgg atcgtgcgga cgcgttaaag ctggcctctg aactgcgtaa
     2641 tgagttctgc attcaggtca cgggcaccgt acgtgcgcgt gacgaaaaaa atattaaccg
     2701 cgatatggcg accggcgaaa tcgaagtgct ggcgtcctcg ctgactatca tcaaccgcgc
     2761 agatgttctg ccgcttgact ctaaccacgt caacaccgaa gaagcgcgtc tgaaataccg
     2821 ctacctcgac ctgcgtcgtc cggaaatggc tcagcgcctg aaaacccgcg ctaaaatcac
     2881 cagcctggtg cgccgtttta tggatgacca cggcttcctc gacatcgaaa ctccgatgct
     2941 gaccaaagcc acgccggaag gcgcgcgtga ctacctggtg ccttctcgtg tgcacaaagg
     3001 taaattctac gcactgccgc aatccccaca gttgttcaaa cagctgctga tgatgtccgg
     3061 ttttgaccgt tactatcaga tcgttaaatg cttccgtgac gaagacctgc gcgctgatcg
     3121 tcagcctgaa tttactcaga tcgatgtgga aacttctttc atgaccgcgc cgcaagtgcg
     3181 tgaagtgatg gaagcgctgg tgcgtcattt gtggctggaa gtgaagggcg tggatctggg
     3241 cgatttcccg gtaatgacct ttgcggaagc agaacgccgt tatggttctg ataaaccgga
     3301 tctgcgtaac ccgatggaac tgaccgacgt tgccgatctg ctgaaatctg ttgagtttgc
     3361 agtatttgcg gggccggcga acgatccgaa aggccgtgtt gccgctctac gtgttccggg
     3421 tggcgcatcg ctgacccgta agcagatcga cgaatacggt aacttcgtta aaatctacgg
     3481 cgcgaaaggt ctggcttaca tcaaagttaa cgaacgcgcg aaaggtctgg aaggtatcaa
     3541 cagcccggta gcgaagttcc ttaatgcaga aatcatcgaa gccatcctgg atcgtactgc
     3601 cgcgcaagat ggcgatatga ttttcttcgg tgccgacaac aagaaaattg ttgccgacgc
     3661 gatgggcgca ctgcgcctga aagtgggtaa agaccttggt ctgaccgacg aaagcaaatg
     3721 ggcaccgctg tgggttatcg acttcccgat gtttgaaggc gacggtgaag gcggcctgac
     3781 ggcaatgcac catccgttca cctcaccgaa agacatgact gctgcagaac tgaaagctgc
     3841 accggaaaat gcggtggcga acgcttacga tatggtcatc aatggttacg aagtgggcgg
     3901 tggttcagta cgtatccata atggtgatat gcagcagacg gtgtttggta ttctgggtat
     3961 caacgaagag gaacagcgcg agaaattcgg cttcctgctc gacgccctga aatacggtac
     4021 tccgccgcac gcaggtctgg cattcggtct tgaccgtctg accatgctgc tgactggcac
     4081 cgacaatatc cgtgacgtta tcgccttccc gaaaaccacg gcggcagcgt gtctgatgac
     4141 tgaagcaccg agctttgcta acccgactgc actggctgag ctgagcattc aggttgtgaa
     4201 gaaggctgag aataactgat atgactcaaa tacacgaaat cattcgcgtt gcatcaaggc
     4261 ggcaactgag tgaactccca tgagcataga taactatgtg aatgggatga gcgaaggcag
     4321 tcaacgaaga ggcagcgtga aggataaagt gtataagcgt cccgtttcga tcttagtggt
     4381 catctatgca caagatacga aacgggtgct gatgttgcag cggcgtgacg atcccgattt
     4441 ctggcagtcg gtaaccggca gcgtggaaga gggtgaaacc gcgccgcaag ctgccatgcg
     4501 cgaagtaaag gaagaggtca ccattgatgt tgtcgctgaa caactgacct taattgactg
     4561 tcagcgcacg gtagagtttg aaattttttc acatttacgt catcgctatg cgccgggcgt
     4621 gacgcgtaat acggaatcat ggttctgtct tgcgcttccg cacgagcggc agatcgtttt
     4681 cactgaacat ctggcttaca agtggcttga tgcgcctgct gcggcggcgc tcactaagtc
     4741 ctggagcaac cggcaggcga ttgaacagtt tgtcattaac gctgcctgaa caggcagggc
     4801 tatctggaga tatttttatg gcaggtcata gtaaatgggc caacaccaga catcgtaaag
     4861 ctgcgcagga tgcaaagcgc ggtaaaatct tcactaaaat cattcgtgag ctggtaaccg
     4921 cggctaagct gggcggtggc gatccggacg ctaacccgcg tctgcgtgca gcaattgata
     4981 aagcactgtc taacaacatg acccgtgaca cactgaaccg cgcaattgca cgtggtgtgg
     5041 gcggtgatga tgatgcaaac atggaaacca tcatctacga aggttacggt cctggcggca
     5101 cggcaatcat gattgaatgt ctgtctgaca accgcaaccg taccgttgct gaagtgcgtc
     5161 atgcattcag caaatgtggc ggtaacctcg gtactgatgg ttccgtagcc tatctgttca
     5221 gcaaaaaagg cgtgatctcc tttgagaaag gcgatgaaga caccatcatg gaagcagctt
     5281 tggaagcagg tgctgaagac gttgtgacct atgatgacgg cgcgattgat gtctacactg
     5341 catgggaaga aatgggtaaa gtgcgcgacg ctctggaagc ggcaggtctg aaagcagaca
     5401 gcgcggaagt ttccatgatc ccgtccacca aagctgatat ggatgcagaa accgcaccga
     5461 aactgatgcg tctgatcgat atgctggaag attgcgacga cgtgcaggaa gtttaccata
     5521 acggtgaaat ctctgatgag gttgcagcga ctctctgatg aggcctgcta aacagcaaaa
     5581 cggagacgcg tgatggctat tattctcggc attgatccgg gttcgcgcgt gaccggctat
     5641 ggcgtcatcc gccaggtagg caggcaactg tcctacctgg gtagtggatg catacgcacc
     5701 aaagtggatg atttaccgtc tcgtctgaag ctcatctatg cgggcgtgac ggaaatcatc
     5761 acccagttcc agcctgatta tttcgccatt gaacaagtct ttatggcgaa gaacgctgac
     5821 tcagccctga aactgggcca ggcgcgcggc gtggctattg tggcggcggt gaatcaggaa
     5881 ttgccggtat ttgaatacgc ggcacgtcag gtaaagcaaa cggtggtagg tattggtagc
     5941 gccgagaaaa gccaggtgca gcatatggtc cgcaccttgc tgaaactgcc cgctaatcca
     6001 caggcggatg ccgctgatgc gttagctatc gcgattaccc attgtcacgt cagtcagaat
     6061 gcaatgcaga tgagcgaatc gcggctgaac ctggcgagag ggcgactgcg ttaagttata
     6121 ccgcctcggt cagttcaggc tgaggcgttt ccactccctc cgcgtgaatc aacaccaaac
     6181 ccggatgatg ccacaaactg gctggcgtga cggttttacg ctcccacgga atagaaccta
     6241 agaaccagaa tttccagaaa gtgagttttg cattcggatt gctatttaac agttgtccaa
     6301 acgtctctat ttcacccacc ggaatacata gcgcctcttt ataaatatca aagacaaatt
     6361 ttgaacagaa ctggcgggaa gattcgtatt taaaacccgt gtggtaaagt ttgcgtagcc
     6421 gggagggaac ctgttcaaca attcgttgtt tctgttgttc tgtcagcccg gcgtctaatc
     6481 gctttatagc atagcgttga ttagcagagc gtttaataaa acgggatagc gtagtgatgg
     6541 ttgagagggg aacacggctt tctgcaacca gaaagtcttc accgttatga ccgataatga
     6601 tcccgacgtg attactccag cagtttgatg cagctgatat ttgaccaaat aaggcagcac
     6661 ctatacatgt aaagacgata tcaccaattt catattcagc agggtaatta atattcacgt
     6721 tgtcattcca ttgaaataga tatgaatata cgtaatagca taaaaaattt attatttcag
     6781 gagtcgcaat gtatattttt attacgcatt tcttcactga atatgtaata ttca
//
LOCUS       DAZFFI010000184         6715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFFI010000184 DAZFFI010000000
VERSION     DAZFFI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.186"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(585..1675)
                     /locus_tag="ACHJ12_004699"
                     /pseudo
     CDS             complement(585..1675)
                     /locus_tag="ACHJ12_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(1938..2126)
                     /locus_tag="ACHJ12_004700"
     CDS             complement(1938..2126)
                     /locus_tag="ACHJ12_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4992372.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            complement(2201..2677)
                     /locus_tag="ACHJ12_004701"
     CDS             complement(2201..2677)
                     /locus_tag="ACHJ12_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4992373.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDMN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            complement(2773..3249)
                     /locus_tag="ACHJ12_004702"
     CDS             complement(2773..3249)
                     /locus_tag="ACHJ12_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO4992374.1"
                     /translation="MQIIIMSRDRYLEYGLMCMLSGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            complement(3312..3518)
                     /locus_tag="ACHJ12_004703"
     CDS             complement(3312..3518)
                     /locus_tag="ACHJ12_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992375.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(3646..4848)
                     /locus_tag="ACHJ12_004704"
     CDS             complement(3646..4848)
                     /locus_tag="ACHJ12_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4992376.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSW
                     NVGVNWEF"
     gene            complement(5114..6442)
                     /locus_tag="ACHJ12_004705"
     CDS             complement(5114..6442)
                     /locus_tag="ACHJ12_004705"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFO4992377.1"
                     /translation="MARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGFENDPVF
                     RVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFSVLALPL
                     GSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKGDDSRER
                     PVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLGDVMDDW
                     NIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSTLSPYQIGEEQITDHRYLLKW
                     RNKNVDYGKHTEGTTGDDSLYGDIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGAS
                     GNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLF
                     LSKDIVTSTDEFISCLKQTGNDVEAGIAGGQIYFVNTALEDINHNSVYIV"
     gene            complement(6447..>6715)
                     /locus_tag="ACHJ12_004706"
     CDS             complement(6447..>6715)
                     /locus_tag="ACHJ12_004706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992378.1"
                     /translation="TGNPASGFGPQLKVVGLFDAQQNLQKIAISFCGTNDICDIVDYT
                     QLNNGKALSEAENLLQSIRDFAISNGLSGEDVIVTGYSLGGWVD"
BASE COUNT         1931 a         1811 c         1245 g         1728 t
ORIGIN      
        1 atgccgcgtc tcatattcat atcctgcaga gaaaacccct ccggagaggg gcgagtgtag
       61 cgtaatggag tgttatgtag catggtgccg ggtgcctccc ggtgaacaga ctgttcgtga
      121 taccagtcag caacatagga aattaatggt atcaccccac cgcacagggg gattcaccat
      181 gcctgccttt tttagcaaat tcatcagaaa ccggacaaca tgcttattta tgaaatgagg
      241 tgcttttcac atttatataa aatcattata acgataatcc taaaaatcaa tgtaattatc
      301 atcttcagta actttctgta ttacaccata cggaaattga tttttgacgg taaagataca
      361 aggggaaata tgtctgtaag gagtaccctg aataatggac aggcatgcat cgtggctgtt
      421 cccctgaaat tatcagtaca accagtcagc tcataatccc tgtctctttt tccggggagc
      481 cctcaggggg actttccctt atccccccag tttctggaga gacgtaaaaa agaattaatc
      541 atatgtcagg tatctgaata tgtcccgtaa ttaatatatt tacgtcagga ttttttctgc
      601 ccgccaggag tatgaacatc ttccgccgtt gagtctgtca tatccatttt tttctgtgcc
      661 agtacttcat ctgatttttc tttttccaga ctctcctgct gcatcccgtg agccggagca
      721 tatacaagct cagccatcac tggaaaatga tctgaaccca tatccggtaa acggcgtaaa
      781 cttttaagca gaaaatgctg gctgtgaaat acatggtcaa gcggccatcg caggaaaaaa
      841 taatggctgt gaaacgtgct aaaaaaacca cgtcccctgc gtggatccag tagtccactt
      901 attttcatga ataaacgagt ggtcgcagac cacgcaacat cattcagatc ccccgctaat
      961 attacaggta attcagaatc agcaacactt ttcccgacca ttaaaagttc tgagtcacga
     1021 tcagttgact catcactttc agtcggactc ggtggaacag gatggagaca atgtaatctt
     1081 attctctgac cttccggcat atgtactatc atatgcatgg agggaatttc cttgtccact
     1141 aaatactgta tttctgcatc acttaaaatc atccttgaat atacatgcat gccatataaa
     1201 ttttctttcg ggcatttcag ggagtatgga aacacttcat ccagaggtga taatttctgt
     1261 tcccaccata agtttgtttc aagaagaaca agtatatcag gtttttcttt atttataagt
     1321 tcaagcaaaa gcatatattt ttattggtca taaggacatt gaataccagt atacttaacc
     1381 ccggaaatcc atcagagtcc tttatttcct ttacctgctt cttgccaatt actgtataag
     1441 gaattatcca taccaactgg taagcgacac aaattaaact tattatcaca acaaacaact
     1501 ctgcaaatga gtcaagaaaa acaacagaca gaaaaacatt caaagtacac agcaaaagta
     1561 tctgtagtcg gggaaaatcc cataccctga caacccatga tgtattaccg gaaacaggga
     1621 taaaagttat gactgccaga aggatagcag taaaaataaa aacacaagtt atcacaaatc
     1681 actccttgtt ctgaaccggc acacaaaact gtcatatacg tttcaaaagt aaaaacacac
     1741 tgctgccaca agattcacga cgtaaccggg cagcatatcc tgattacgga caatccatga
     1801 aaccgcctca ccagaagcgt ccatcacatc cgttttttcc ctgttttata ttccacgaac
     1861 cattttattt tcaggaatct ccgggccttt atcccgcatc atatacacag ctatgacaac
     1921 ctgctcttct gcagtcatca tttccccgta gcttcgcgga ttttctgttc cgtcttatac
     1981 tgcgaaagcg catacacggc ccatattgtt gccggtatcc agcccagcag cgttatctgt
     2041 aacaccagac aaaaaatccc cgtcagaggc cggccaatgg tgaaaaaagt cagccaggga
     2101 agaagaaaag caatcaacag acgcataaaa actccttatg attatgaaat taacgaatca
     2161 gaggctgcgt ttaacattac cggaaataag ctgccctgct tcacaacctc ccttttctga
     2221 ccagaagcat ccccttgtac aacaactcaa cttcattatg tacccgcaac ctctttatca
     2281 gattctgctt atgagtggag aatgttttct ctgacatgcc aagatatcgt gccccctctg
     2341 ttccgttact gccataaagc agcatcatca gtacatttac ctgccggcct gacagggtca
     2401 gcttctccac cgacatgaat gcgcggtaca gatgactgat atcgtttact gaacatcgct
     2461 cagtaagatg tctgatatca taaaccggac tgtcaaccac tccccagaga aaacggagcg
     2521 cttccggaga agaaaaataa agggtgttca tatcacttat attattctta tacagaaaac
     2581 cgacaatttc agatatccgg gaagaagata ttatcaccac attatctgtc tgcatgccgc
     2641 tgataatgaa tttaccggga ttaatatact ccttcattcc ctccagaaaa taactgttat
     2701 cacaaataaa atgcataccc gttccacatg aaatttatca cgcctggcca cataacatta
     2761 attatcccgg atttaccggt ctgcggtgct gcagagatat ttaatactga cgccgcgcag
     2821 accatttttc tccatcgcat tgtaaagatg cgcacatacc gtcttcatgc ttatccccag
     2881 ctggtctgcc agaaaggtaa aaccgtactc atggaaaaca actccgaaga ccaccaccat
     2941 ctcggtacgg gtcagtggtc ttgccgtatg tccgaacagc cacgcaccac gacggatggc
     3001 ctgcctgata tctgtcaggc atctcacaga ggaaacagga atgacaagaa aacgacgccc
     3061 acagaacata cagtatgtaa gcctttccag attacggtca cagagaatca cataactgtc
     3121 ttcgggaagt ggcagacggc gctttccggc atcaaacagt tcacggcctg tcgtgagcct
     3181 gtatccgctc agcatacaca tcaggccata ttccagatat ctgtcacggg acataataat
     3241 aatctgcata ctctcagccc cttatctccc ggataccggc tccgtttacc attaccggta
     3301 taactgactc atcattcata acggaaactg tctgccgcat acatcagtgc ccgctcttcc
     3361 agctctgaca gactcagctc tttcagttcc ccttctcctt ccgcaagaag acaacattca
     3421 aaaacagctg catcgtcgca ttccagacga acctgcaccc tgtcagcccg accgtttaca
     3481 gtaaatacct gcactgaact tatgcgatag ttcagcatat acactctccc tctcagctcc
     3541 tcaggaatac cccggaaccg tcagtcatgc caccggggag aaaagcaggc agcgaatcat
     3601 gaccgcatac agaacgctgc ccgccccggt gactcagacc cgggttcaga actcccagtt
     3661 gacacccaca ttccaggaaa catcaccgtc actgaacgca gtacctgcct ttgccgcagc
     3721 ctgttcgtta aagcgatatc ccatgcccac cgcaacagcc tgcttgtcac tgtacccgcc
     3781 cacagccgct gtggcgttaa atttacccac actgtatggc tggaaaagac cggccagcgc
     3841 tgctgactgg gctgctgccc gcttcatttc ctcatgattc tcattaatct gacgctgctg
     3901 actgttcata cgacgctcat gcgcctgcag ggtacgtgcc tgagaagcaa tggcgcgact
     3961 gttgcgttca cccacctgac ggttacggct taccgcagca cggttttctg tggccatttc
     4021 ctgtgtacgc accagccctt cctgtacagt aattcgttca ccggttccct caccgcgcgt
     4081 cacataacca tgactgtcca cagcaacggt acttccggta tttatctgct gttgctggct
     4141 gttcaggcgc tcttcatgcg tctccagcac gtctctctgg gaaagcagcc ccctggtgtt
     4201 acggtttatg gccaccgtgt tccggataat attctccctg ttctctgcag caaccgtatc
     4261 ggtatggtca cgcgccttaa gtgcttccgc tgcaatccgt ccactgacgt cactgtacac
     4321 ctcatccgcc ttcctgttca gttgcccgac agtggctgca tccgtatccc gtgtaccatc
     4381 actgacacca ccgagaaccc gggttttatc cggtacacgc ggactggccg ggtttgtgac
     4441 acctgagtca tatccgatgt agacttcatt ttcacgggtt gtaccggact gctgaccaag
     4501 agccactgaa tacctgtgca ttgcctgtgc acccgtaccc agtgctactg aatttaaagc
     4561 atctgcagat gcccagctac ctactgccac accattagac gcctccggat ttacataagc
     4621 agacatcccc acggatgtgg aaaacggcgc atgcgcatac gatgcagagc caagagctgt
     4681 ccctgccttc ccggcattcg cccatgcccc cactgccgtg gagtcagtct ctgatgcaga
     4741 ggctgtgtcc ggagacgccc aggaactgcc atcctgcgca aacgccggag caccggcaat
     4801 cagaaaggaa ataagcgata aagtaatacc gtgtcgattt attttcataa acaacctcat
     4861 tgatatacac aatatactgg cacagagtta aattaatata taatcaggag agaagtgaaa
     4921 gagtacccgg tgcgaaaatg aattaatctg agcagattta acggtaaacg aagcgcaata
     4981 aattaaaatt aaaaaacaaa atcacttgca gtctattatc tttacgttcc tcaccgcaac
     5041 cgcacacaaa aacaaaacac acaatgcatt gattttaaac aaaaaaacaa aacagaaact
     5101 caacacataa aaatcagaca atataaacag aattgtgatt tatatcctca agcgctgtat
     5161 taacgaaata tatctgtcct cctgcaatac ctgcctcaac atcatttccg gtttgtttca
     5221 gacatgaaat gaactcatct gtcgatgtaa caatgtcctt tgataaaaac agaaaatctt
     5281 tttcaccctt atgtttatta aaatccatga taaatgaatt cccttcacaa gcagcatcaa
     5341 agacgaagac atcatgacca gtaccaccat acagataatc attacctccc ccaccatata
     5401 accagtcgtt accttctgct ccgtacaact catcattacc agaagcccca tgaagataat
     5461 cattcccgcg acctccgtgt aaaagactta ctccttctcc cccggcacta tgtaatatat
     5521 catttccgtc aaggccaaaa attatatctc cgtacagact atcatctccg gttgtacctt
     5581 cagtatgttt gccataatca acatttttat tacgccactt cagaagatac ctgtggtctg
     5641 taatctgctc ttctcctatc tgataaggac ttagtgtact cctccagtca ccagaaaaag
     5701 agataccttc aatacctgtt gcctcagtaa gaccggctcc ccgttctgag tggaaaaaca
     5761 gtgtcccgtc actgaccctg tatatattcc agtcatccat tacatcgcca agaagaagcg
     5821 tgtcataccc actgccacca tcaaccctgt ttgctccctt acctggttca atcttatcat
     5881 tccctcctgc cccctctata tagtcaccac cctgcccacc tgccagcaga ttattgccat
     5941 cattaccgat gatgaataca ggtctttcac gactgtcatc ccccttgtca tgaacccatg
     6001 aataccagga tgtccagcca ctctgctcat ccacaatgac tgtactgtcc ggtgacatgt
     6061 agtcgtaaaa ctgagaccct aaaattcttt caaaggcgtc agtttttgaa cccgcaaaat
     6121 gcgcactcca gcttcccagc gggagagcaa gcacagaaaa ggggataata ttccagagga
     6181 atgaaccata aaggtctgtg tataaaacaa tgttatcagg agaagagtca tattcacgat
     6241 ccggggttgt caagcccggc cccattgctt taactgcctc ccagaacccg gctccatctc
     6301 ccagcacacg aaataccgga tcgttttcaa aaccgatatt taataccttc ccatccccgt
     6361 caaataccag cggggaagcc caggctatat agtcagaatt agcataaaaa ccatcagcca
     6421 gagactcact gagtctggcc ataatgttag tcaacccacc cccccaggct atagcctgtg
     6481 acaataacat cttcaccact taaaccatta ctgattgcaa agtctctgat actttgaagc
     6541 aggttttctg cttcagacag cgcttttcca ttattgagct gtgtataatc aacaatatcg
     6601 cagatatcat ttgttccaca gaatgatatt gcaattttct gtagattctg ttgagcatca
     6661 aacaacccaa caactttcaa ctgaggacca aatcctgacg cagggtttcc tgtaa
//
LOCUS       DAZFFI010000185         6671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFFI010000185 DAZFFI010000000
VERSION     DAZFFI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.187"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(205..450)
                     /locus_tag="ACHJ12_004707"
     CDS             complement(205..450)
                     /locus_tag="ACHJ12_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992379.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASRKREAALAAGLEPDDTFTWQPQRRR"
     gene            714..2807
                     /gene="cda"
                     /locus_tag="ACHJ12_004708"
     CDS             714..2807
                     /gene="cda"
                     /locus_tag="ACHJ12_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFO4992380.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDMKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVISETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNSLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVITKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2804..3067
                     /locus_tag="ACHJ12_004709"
     CDS             2804..3067
                     /locus_tag="ACHJ12_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO4992381.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3137..3283
                     /locus_tag="ACHJ12_004710"
     CDS             3137..3283
                     /locus_tag="ACHJ12_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO4992382.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSPKLIG
                     VAVQ"
     gene            complement(3502..3616)
                     /locus_tag="ACHJ12_004711"
                     /pseudo
     CDS             complement(3502..3616)
                     /locus_tag="ACHJ12_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            3990..4178
                     /locus_tag="ACHJ12_004712"
     CDS             3990..4178
                     /locus_tag="ACHJ12_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992383.1"
                     /translation="MDNGVTAFEKGESARRSRTTERSESVSEEAEEHRKRHFLLTHLC
                     GISHPAWCTFHTILTDAT"
     gene            complement(4330..4521)
                     /locus_tag="ACHJ12_004713"
     CDS             complement(4330..4521)
                     /locus_tag="ACHJ12_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4992384.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4589..4912
                     /locus_tag="ACHJ12_004714"
     CDS             4589..4912
                     /locus_tag="ACHJ12_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO4992385.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5121..5555
                     /locus_tag="ACHJ12_004715"
                     /pseudo
     CDS             5121..5555
                     /locus_tag="ACHJ12_004715"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5590..6150
                     /locus_tag="ACHJ12_004716"
     CDS             5590..6150
                     /locus_tag="ACHJ12_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO4992386.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWELIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6157..6390
                     /locus_tag="ACHJ12_004717"
     CDS             6157..6390
                     /locus_tag="ACHJ12_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO4992387.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1666 a         1434 c         1939 g         1632 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc acccttagcg gcggcgctgt ggttgccagg tgaaggtgtc
      241 gtccggctcc agtccggcag ccagggctgc ttcccgcttt ctgctggccg ttcgttcatc
      301 gagaatggct tttagtatcc gtgcccggag gcgtcgcttt gtttcctgtg gcattttgtc
      361 ctttttcgtc agccccatgg cacgagcttc agcggcaatg gcttctgcca gtcggcgctc
      421 ttcggcttcg gtcagtctat ggcgtttcat ggtggtctcc gggtgtttct ctggtgtcat
      481 aacagtttaa ctgttggctc tctccttatg ctaatcagcc ttctttcatg cttgttgcca
      541 ttgagtttaa gaatgattgg ttgtttttct tgagcgtttt cacaaaaacg taaatccagt
      601 gggttgacag gatttacgag gtgacgtaac ttttcactgt acataaaacc agtggtttta
      661 tgtacagttt attgttttta acgcattgtt ttaaaagtca aagaggtgtt tttatgagtg
      721 attacgaagg tagtggtccg acagaaggta ttgattatgg gcactcgatg gtcgtgtggc
      781 cgtcaacagg actgatttcg ggtggtgata tgaaaccagg aggctcatca ggtatcgctc
      841 catccatgcc tccgggatgg ggggattaca gcccacaggg tatcgcactt gtacaaagtg
      901 ttctttttcc gggaattatt cgccggatta ttctggataa ggaacttgaa gagggagact
      961 ggtcgggatg gtctgtcagt gtgcatagcc cctggggaaa cgagaaagtt tccgctgcac
     1021 gaacagttct tgaaaatggt ttacgtggtg gtttgccaga accgtctcgc ccggctgctg
     1081 tttcttttgc ccgtctggag cctgcttccg gaaatgagca aaaaattatt cgtcttatgg
     1141 ttacacagca actggagcag gtaacggata tccctgccag ccagttacca gcagcgggta
     1201 ataatgtacc ggtaaaatat cgtctgacgg accttatgca gaatggtaca caatatatgg
     1261 ctattatcgg aggtattccg atgacagtgc cagtagtgga tgccgttcca gttccggacc
     1321 ggagtcgtcc gggaaccaat attaaagatg tttacagtgc ccctgtatca ccaaatctac
     1381 cggacctggt attaagtgtg ggtcagatga atactccagt tcggtctaat cccgaaatcc
     1441 aggaagatgg cgttatttct gagacaggga attatgttga ggctggttat acgatgtcca
     1501 gtaataatca tgatgtcatt gtccgttttc ctgaaggcag tggtgtttct ccgttatata
     1561 tttcaactgt agagattctg gacagtaata gtctgagtca gcgtcaggaa gccgaaaata
     1621 aagcaaagga tgatttcaga gtcaagaaag aagaagcggt agcccgggct gaagctgaaa
     1681 aagcaaaagc cgagttattt agtaaagcag gtgtgaacca gcctcctgta tatacacagg
     1741 aaatgatgga aagggctaat tcagtaatga atgagcaggg agctcttgtt ctgaataaca
     1801 ccgccagttc tgtacaactg gcgatgaccg gaactggtgt ctggactgct gctggtgata
     1861 ttgcaggcaa cataagcaag tttttcagta atgctctgga gaaagtaacc atacccgaag
     1921 tgagtcccct gcttatgcgg atttctcttg gtgctctgtg gtttcattca gaagaagctg
     1981 gagcaggaag tgatatcgtg ccggggcgaa atctggaggc gatgttttcg ctgagtgctc
     2041 agatgttggc tggacagggc gtggtcattg aacctggtgc gacgagtgta aatctgcctg
     2101 ttcgtggaca attgataaac agtaacgggc aattagctct ggatttactg aaaacaggga
     2161 atgaaagtat cccggctgcg gttcctgttc ttaatgctgt ccgtgataca gcaacaggac
     2221 tggataaaat cacgttacca gcagtagtag gcgcaccttc ccggacgatt ctggttaatc
     2281 cggtaccaca accttcggtg ccaacagata caggtaatca tcaacccgtt ccggttacac
     2341 cagtgcatac aggaacggaa gtaaaaccgg tcgaaatgcc agtaacgacg attactcctg
     2401 tttctgatgt tggtggacta cgagatttta tttactggcg tcctgatgct gctgggactg
     2461 gtgttgaagc tgtttatgtg atgcttaatg atcctctgga ttcagggcga ttttcgcgta
     2521 aacaacttga taaaaaatat aaacatgctg gtgattttgg tattagtgat acaaaaaaga
     2581 atcgtgaaac tcttactaaa ttcagggatg ctattgagga gcatttatcg gataaggata
     2641 cagtagagaa aggaacatat cgaagagaaa aaggttcaaa agtttatttt aatcctaata
     2701 cgatgaatgt ggttataact aagtcaaatg gtgagttctt atctgggtgg aaaataaatc
     2761 cagatgcgga taatggtcga atttatttag agacaggtga actatgaaca agatggcaat
     2821 gatcgatttg gcgaaattat ttttagcttc gaaaattaca gcaattgagt tttcagagcg
     2881 aatttgtgtt gagcggagaa gattgtatgg tgttaaggat ttgtctccga atatattaaa
     2941 ttgtggggaa gagttgttta tggctgctga gcgatttgag cctgatgcag atagggctga
     3001 ttatgaaatt gatgataatg gacttaaggt tgaggtccga tctatcttgg aaaaatttaa
     3061 attataatca tgagtgattt taatatctgg catttaagta taaatgtcag atattcactc
     3121 atgacaagga gatgtcatga aaaaaaagat agggggatct atgattctcg ttcttgctgt
     3181 tttgtgtctg actgcttgcc aagctaacta tgtaagggat gttcagggag gaacaattgc
     3241 tccatcatca tccccgaaac taatcggggt ggcggttcag tgaaaaatat taaaggatct
     3301 tcttgagatc ctttttttct gcgcgtaatc tgctgcctgt aaacgaaaaa acccgccttg
     3361 tggcgggtcc ttccgtccga tcagaagtct tcgacctctt ctaatcggcg gtggtaactg
     3421 gcttggagga gcacagtcac caaaactgtc ttttcagttt agccttaact ggttcataac
     3481 ttcaagagca actcctctaa atcagttacc aatggctgct gccagtggcg ttaagtcgtg
     3541 tcttttccgg gttggaccca agagaacagt taccggagaa ggcgcagcag tcgggctgaa
     3601 cggggggttc ctgcatacag cccagcccgg agcgaactgc ctaagtcgcc ccgagatacc
     3661 agaagacgtg agctatgaga aagcgccacg tgtcccgcaa gggagaaagg cggaaaaggt
     3721 atccggcaat cgatatggcg ggaacgtggg ggcgcaggag ggagcgattc accggaaacg
     3781 gtgatgatct ttaagccacg tcgggtttcg ccaccgctgg cggatttcat ggttgagccg
     3841 taaggctccc acagatgcac cgaaaaagtg tctgttttgt gaagtccgtc aggggggcgg
     3901 agcctatgga aaaagcgcct ccggcgcggc cttttccggg gtttttgttt tttgcgcaca
     3961 tgttctttcc cgggttatcc ccggattctg tggataacgg ggttaccgcc tttgagaaag
     4021 gagagtccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     4081 aagagcaccg gaagcggcat tttctcctta cgcatctgtg cggcatttca cacccggcat
     4141 ggtgcacttt tcatacaatc ctcactgatg ccacatgact aagccagtat acactccgct
     4201 atcgctacgt gactgggtca gggcttcgcc ccgacacccg ctaaaacccg ctgacgcgcc
     4261 ctgacgggct tgtctgctcc cggcatccgc tcacaggcca gctgtgaccg tctccgggag
     4321 ctgcatgtgt cagaggtttt caccgtcatc cccgaagcgt gcgaggcagc tgcggtaaag
     4381 ctcgtcagcg tgatcgtgaa gcgcttcgca gatgtcagcc tggtcgtcgg cgtccagttc
     4441 attcagtttt tccagcaggg tcagcgtctg gcttctgatg aagcgggcca tgttaagggc
     4501 ggttttttcc tgtttagtca cagttctgtt tctccatgtg tggggagtct gtcggaggtt
     4561 tttaatgata ccggtgaaac gcgaaaggat gctcacgata cgggtcacgg atgaggaaca
     4621 tgcgcggctg ctggcacgtt gtgagggaaa acaacttgcg tcatggatgc gtaaggtctg
     4681 cctgggggca ccgccatcga aaacgtcagg tcttccgaca ctggcaccgc cgctgttgcg
     4741 ccagttcgct tcggtcggca ataacctgaa tcagatagcc cggaagataa acagcggaca
     4801 gtggtcgggg catgaccggg ttcatgtggt ggctgcactg atggccatcg gacgtgaact
     4861 gagtgagctg agggatgagg tccgtaaaca gggagaacgg gatgatagtt aagtttcatg
     4921 ccaggggaaa aggcggtggc agtggtccgg ttgattacct gctgggccgg gagcgtaacc
     4981 gggaaggggc gagagttctg cggggtgctc ccgaagaggt gcgggaactc atcgatgcca
     5041 cgccgtttgc gaaaaaatac acctcggggg ttctgtcttt tgcagagcag accctgccac
     5101 cgggagagcg tgaaagggtg atggagagct ttgagcgggt actgatgccc ggtcttgaaa
     5161 aaaatcagta cagcatcctg tgggtggagc accaggacaa ggggcgtctt gagctgaatt
     5221 ttgtcatccc gaatatggag ctggcgagcg gaaaacggct ccagccctat tacgaccgag
     5281 cggaccgtcc gcgaatcaat gcctggcaga cactggtgaa tcaccattac gggctgcacg
     5341 acccgaacgc accggagaac aggcggacac ttgtcacccc gaataatctg ccgaaagcga
     5401 aacaggaggc cgcagaagcg attacgaggg gactggaggc tctttaccat gccggagcgc
     5461 taaaaacgcg tcaggatgtc actgaggcgc ttacagcggc aggttttgag gttgtgagga
     5521 caaccaggag cagtatcagt atcgccgacc cggagggtgg gcgtaatctc aggctgaaag
     5581 gggcgctgta tgagcaatct tttacaaatg gggacagact tcgagaagaa actgaaagag
     5641 cgtgccgtct ctacagagaa catgctgaac agcgagttca ggaagctcga agcgtctgta
     5701 gacagggaac tgaacttaaa cgggcagaaa atcagagacg ctatcagcgc acacacagca
     5761 gcggtgaaag aacaactgga gacgctgaac agtgccgtga acgcgcagtt cagcgccaca
     5821 gagaacgaac tggcccggcg gcaggaagag ctgctctgga agctggtcaa agggcggata
     5881 ctgtacccgt cgctgacggc gctttgcgtc acgggcggga tttttctggc gtcatgggag
     5941 ctgattcagt ggcaggaaag ccggattgcg gcgaacatcc tggctatcag ggaccaggaa
     6001 gagacgctgg cgaaactccg ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac
     6061 gggaaatttc tggtgctgcc gtccggggtg aagggggaaa acaactggac ggtggagaag
     6121 aagaacgcag taagactggt cagggagtaa gtgagtatga cagagctgga aatgcactta
     6181 ctgaacgcct tagagcaatt gcagcaggac tatacgcagc ggctgaaaga ctgggagagc
     6241 gccttcacgg atttgcagca tatgttcgag ctgacacgac aggagaacgg gcaactgagg
     6301 gaacagtgcg acgacttgag tcagcaggtg cagagattag ccgggcaggt ggaccgcttg
     6361 agtcggttat ttatcacgaa cagcagataa gggatgaaca ggaaaggcag gcacgcctgg
     6421 aacgggagcg tgcgcggtcc tggcatgaag agcaggaacg ggaagcacag gtatggcgtg
     6481 gcccgtcaat ggggatgtga cggtgagttg ttccgggagg ttgccagatg gtaccggaac
     6541 gcgttaaaaa cgcatccggg cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct
     6601 ctttgccaga tcgcgctcat tcctcagcct catcccgcga tgtctgcgta tgtcatcaac
     6661 tgaaaagatg g
//
LOCUS       DAZFFI010000186         6666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFFI010000186 DAZFFI010000000
VERSION     DAZFFI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.188"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..1358
                     /locus_tag="ACHJ12_004718"
     CDS             174..1358
                     /locus_tag="ACHJ12_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4992388.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            1687..2730
                     /locus_tag="ACHJ12_004719"
     CDS             1687..2730
                     /locus_tag="ACHJ12_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4992389.1"
                     /translation="MNDRLRFEVNDNQGCFVFPDAWFGSLLDEFDELIDAYDTDEISE
                     TSYINKLRRLARQEPDFIDVHAYLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAALLTDKILAYNPEDNQGARWLLGPEL
                     LRTGDHEQARHVLKAHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            2873..3102
                     /locus_tag="ACHJ12_004720"
                     /pseudo
     CDS             2873..3102
                     /locus_tag="ACHJ12_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3089..4591)
                     /locus_tag="ACHJ12_004721"
     CDS             complement(3089..4591)
                     /locus_tag="ACHJ12_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4992390.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTDIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(4753..5499)
                     /locus_tag="ACHJ12_004722"
     CDS             complement(4753..5499)
                     /locus_tag="ACHJ12_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO4992391.1"
                     /translation="MKKIIAVSALAVVGMFSGQALADRGKTGFYMTGKAGASVVTQTD
                     QRFCQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDAS
                     VSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            6289..>6666
                     /gene="subA"
                     /locus_tag="ACHJ12_004723"
     CDS             6289..>6666
                     /gene="subA"
                     /locus_tag="ACHJ12_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO4992392.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSW"
BASE COUNT         1889 a         1582 c         1486 g         1709 t
ORIGIN      
        1 tcacttcctg attcatctcc cgaactccac gaaactcaag tataatcaca tcgttgcaag
       61 caatctgaac attctgaact ccaccagact ctactgaacc ctctctcaat caagcgttca
      121 ttgggggccc cggtgggggc tcattgtgtt cagtcagttt caggagcccc catatggcac
      181 ttacagccag acaggtcgaa acagccagac ctaaagaaaa agactataaa ctctctgacg
      241 aacgtggttt atatctgctg gtaaaaacca cgggagcccg ctactggcgg cttaaatacc
      301 ggatagcagg aaaagagaaa aaactggccc tcggcgtcta tcccgacgtc tcccttgctg
      361 aggccagaat caaacgcgac gatgcccgaa aaatcatctc cgaaggtggt gacccgggcg
      421 aaaagaagcg aaaggaaaaa ctcactcaga aaatctctgc caccaatacg ttccatgccc
      481 tcgctacgga atggcaccag cataaatctt tgtcatggtc tgaaagttac gccagaagcg
      541 tactggaagc gctggataaa gatattttcc cgtatctggg caaacgaagc gttacggata
      601 tcctcccgct ggaaatgctg gaaattctgc gccgcataga aaaacgtggc tcgctggaaa
      661 aactgcgtaa ggtccgtcaa tactgtaatc agatttttcg ttatgccatc gccaccggac
      721 gagccactgt caatccggca tctgaactga ccagtacact ggcggcgcca aaagcggcgc
      781 atttccccca cctgagagca gatgagctcc ctgtttttct caggaagctc gctgaatatc
      841 atggcagtcc agttacccgc atggcgacaa acctgctgct tctgactggc ctcagaacca
      901 ttgaactacg gtctgctgaa tggtcagaaa ttgattttga taatgccctg tggacaatcc
      961 ctgaaagccg catgaaaatg cgacgtaaac atgtcgtacc actgtcacga caggtcactg
     1021 acattctgca acaactcaaa actttctccg gacaataccg gctggttttc ccgggacgtt
     1081 gtgatatcaa caagccaatg agcgaagcca gcatcaatat ggtgctcaaa cgtatcggtt
     1141 acgatggcag agcaaccggt catggttttc gtcacaccat gagtaccatt ctgcacgaac
     1201 aggggtttaa ttctgcctgg attgaaatgc agttagctca tgtggataaa aattccatca
     1261 gggggaccta taatcatgcc ctgtatattg atggtcgccg tgaaatgatg cagtggtatg
     1321 ctgactacat tgattcgctt tctgagcagg taagttaagc tgagaacacg ttattctgat
     1381 tttcacttac ttcacgatta ttcctccggc ctgcaggttc ggaggattct tatttttatg
     1441 ttttattttc agataaagtt tttgacaaaa cgtttattct gcatcagcaa aattatcaca
     1501 aaacaacgca atcacagaca aattaacatt tttcagacgg gtatattgag tgcccacaat
     1561 gtaagacagg caggcatccg gtgacatggt aaaatattaa tccagggcaa aaacaaggct
     1621 tgatacacaa catcatggct tttatgctct cagactatgc caccacagat tttacaggct
     1681 cagaaaatga atgacagatt acgctttgaa gtgaacgata accagggctg tttcgtgttc
     1741 ccggacgcct ggtttggctc gttgctggat gagtttgacg aactaattga tgcatacgac
     1801 acagatgaaa tcagcgaaac aagttatatc aacaaactca gacgccttgc ccgacaggag
     1861 cctgacttta ttgatgtaca cgcttacctg gcatacgcat ttctggagca gaatgccccg
     1921 cgtaaggcac tgaacgctgc tcttaaagga ctggcagcag gtaatcgtct catcccggaa
     1981 agtttcagtg gtgaaatcat ctggatgcat cccgaaaacc ggccttatct gcgcgcgctt
     2041 tatgcagcaa ttctcgcaaa tgtgcactta cagcgtcatc aggatgcagc tctgctcact
     2101 gataaaatac tggcatacaa tcctgaagac aatcagggag cacgctggct actgggaccg
     2161 gaactgttac gtactggcga ccatgaacag gcacgccatg tactgaaagc gcatgcagat
     2221 gaattttcac cttactggta tgaactcggc cttctgcatt ttcttaatgg ggagctggta
     2281 aaagctgcaa ctgcatttcg tcgcggcttt gcggcaaata cttacatcgc agaaatactg
     2341 tgcggaaatc tgcatccatt tccacttgct gtatggcata acttttcagg cggtccggat
     2401 actgctgaag attattatgc cacataccac ccgttatggg ggcaatatcc ggaagcattg
     2461 ctgtttgtaa actggctcta taatcattcc tctgtgctgc atgaacgggc agaaatcatt
     2521 aaatgcgccg aaatgctgat gcaggaagat gatttcgaaa tatgcgaaag tattttaaga
     2581 cagcaggaga agttgcgtga aagaattgat gagacgcttt ctgagaaaat tgtacagaaa
     2641 tgcagaaata tgaatggtga atatgtctgg ccctggatat tgccgttttc agcggcaggc
     2701 atgaaacata ctggcataca gtatcagtag atattgcatt agtgtatcct gcacacaagt
     2761 aataatttat ccaccaataa taacactgtt aatgtcccct tcccctggtt gtcagccagg
     2821 ggttatcttc tgaatatttc ttttgaaaag gataacacag taaattattt ttatgaatta
     2881 tcccatggac tcattaacac cctttcataa tgctttattg tcaaacacgt tatggctgac
     2941 atcaaaaaaa ccggatttcc tctgccagcg ggtaatcacc tccccggtgt tttcggttgg
     3001 tctggttact ccagtctggt aattagcaag ataatcgata taaacagtgg aaaactcatc
     3061 gtacataatc tggtgatgaa cattacgctt attttcccct gaccggaaga atcagaggct
     3121 gcggtttcag actgtctgcc ggtacattcc tctccccgtt aaaaaccata acgggttcat
     3181 tatcttcgtc tgtcagcgga ttgaatggcg gtatattttc agtacgaatg tcggtcagcc
     3241 actgaaaaat acctgcgaaa tgacgggcac tgattttcct gctgacggac tgatgagacg
     3301 tgatgtcact ggcggtaata atcaggggaa cgctgtagcc tccctgcaca tgaccatcat
     3361 gatgaacagg attggcacta tcgctgaccg acagcccatg gtcagaaaag taaagcatgg
     3421 caaaatgacg ggaatgccgg cgaaggatac catcaagctg accgagaaag ttatccagtt
     3481 tactgatgct ggcgaggtaa caggcaatct ttcggggata ctgctccagg taatgattcg
     3541 gccaggagtt aagtcggtca cacggattcg gatgagagcc aatcatgtgc aggaaaatca
     3601 ccttcggaga ggatttatcc gccagcgcac gttctgtttc ctgtaacaac aacatgtcat
     3661 ccgttttacg ggaagcgaag ctgcctttct tgaggaaaac ggtatgctcc gcatcagaag
     3721 caataacaga gatgcgtgtg tcatgctctc ccagttttcc ctgattggat atccaccatg
     3781 tgctgtatcc tgcttttgct gccagcgcca ccacgttgtt gccggaatca gggttctgct
     3841 catagtcata aatcagtgtc cggctcaggg aaggtacggt actggctgct gccgatgtat
     3901 agccgtcaat aaataaaccg ggagccgtat tcagccacgg tgtggttggt acgggataac
     3961 catataccga catataatcc ctgcgcacac tctcaccagt gacaatcaca atcgtgtcat
     4021 acaacggtgc tcccggcagg attttccagc tgtcagccct gtgctgactc agttgttcat
     4081 aacgctgcat ttcacgcaat gtgtcagttg tccccacaac ggttccttta accatccgca
     4141 acggccagct gtttacagag cataatacga acagcagcag tgccagccag ttacggtgac
     4201 cgcggtggtg tgtccgccag aaaataacca tgaacaccag aatcgcggca ctgaccagaa
     4261 aatgataaac aggaatcatc ccggtaaact ccgcagcctc atccgccgtg gtctgcaaca
     4321 acgcgacaat aaaactgttg ttgattttac cgtacgtcat acccgcaggc gcatacagtg
     4381 cacaacagaa cagaaataac agagctgtaa tggatgtaag ggtatttctg tgtgcaagga
     4441 gcagaagaag gaacagcagc aacacattcc cggtggcatt cttctcagtg tatccgcatg
     4501 caattgtggt tatgacagaa ataacaaaaa agaataaaaa caataaaatc cgtggtgtgt
     4561 tgtccggaca aaacagtttt ctgatattca tcggagtata tcggcaacat tattatgaag
     4621 agaataaaat aataagaaaa acagaaatat cctgtaaagc agataacaga caaccctgca
     4681 gtataattac ggcagggagt ttccgtttaa ttacagaaga caggtaatta ttatctttca
     4741 ggagtagttt cgtcagaagt tataagtcac accaagcatg atgtcatgac ttttaacatc
     4801 aacttctgat ttatatttat cgccccactc atccttgtaa ctcacactgg catcacctgc
     4861 atcaagatag cgatagctga ggtccagagc gatatccggg gttacgtcat agcggacacc
     4921 tgccccaaaa ctccatgcaa aattgttatc atatcccggg taatgcaacg cagaaaaatc
     4981 gctcacctca ccagatttat tccaggaggt atcagtatat gttgccttat gatgtatccg
     5041 tgcataaccc agtccaacag acacccatgg agtgaatgca ctgctgtttc tgaagtcata
     5101 atacgtattc accatcagag tattcacact gagcctgttc tgtgtgtctt cccgaccacc
     5161 accgaccatc ggagagtacc atgtatccag tgtataacgg gagtccgcag ctccgcggcc
     5221 atagaattcc acttccgtgc gcacaggaac attgtaatgc tgataaaaat catagcccac
     5281 cgcaaggccg gcaccgaata ccgtatcgtt cttatcgccg cccttatact tataaacatc
     5341 gtccccaaag tcctgacaaa agcgctggtc agtctgagtc acaaccgaag caccagcttt
     5401 accggtcata taaaagcctg ttttgcctct atcagccagg gcctggcctg aaaacatgcc
     5461 caccacagca agcgctgaaa ccgcaataat ctttttcatt tcagttatct ccgctatcac
     5521 tttccccgaa cgataaacgt ggttgcttat tctaaagcta tgcaaaagac agggtaaaaa
     5581 gagaaaaatc gatcgaatcg atcgacaaaa tcgatcatgc atgatgaaga tttcttatcg
     5641 aatacataaa aatagtgatg gcaatcgtgg caggagctta cgtcaggccc tgaagtactg
     5701 acacaggcat tgatggcagg tgatataagg agtgctccag ggagtgactc ccgactgacc
     5761 caggcactgt cccgttgtca acccgtatgg ttactgctct gactcatttc ctgtcgtcag
     5821 acgttcaact ctggaccact ggttatgacg acaggtgtcg ctcatttgtc agcactataa
     5881 cggtcaaaga tcgtggcatt gacacattct ccagaggtaa atttctcaag aggtaacaac
     5941 tctgccagga ggatatctgc agagagttac attcgtttgg gttcatatct gtgccgcgcg
     6001 ttttccttcc atcagtgaac aaacattcag tcccatgacg accgggcaaa tagcgtgttc
     6061 acagatattg ctgtccaggt acgaagtacc ataatggata ttggtaagtt attgggtgaa
     6121 ccccctccat gaaaaaacca aatataaaaa tgaatgtttc gggagagact tcactcgctt
     6181 tattgatagt gattgtttat cttttactga agacaggata tttcagatgt gatatccctg
     6241 taacatattg accagcaata ctttttattt gtaccactgg aggatattgt gctgaaaaat
     6301 ttacggctgc atattctgtt cctgcttttt atctcagtgt cagtcagggc ggaaaaacca
     6361 tggtattttg atgcaattgg tctgacagaa acaaccatgt cgctcacaga caaaaatacc
     6421 ccggttgtcg tatctgttgt tgattccgga gtggcatttg taggaggcct cagcgacagc
     6481 gaatttgcca aattcagttt tactcaggat ggttcgccat tcccggtaaa agagcctgaa
     6541 gctttatata ttcatggtac tgccatggct tcactcattg cctcacgtca tggggtttat
     6601 ggtgtttatc ctcatgcact gatatccagc agaagggtta ttcctgacgg tgtacaggac
     6661 tcatgg
//
LOCUS       DAZFFI010000187         6643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFFI010000187 DAZFFI010000000
VERSION     DAZFFI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.189"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(370..1296)
                     /locus_tag="ACHJ12_004724"
     CDS             complement(370..1296)
                     /locus_tag="ACHJ12_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4992393.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
     gene            1835..2710
                     /locus_tag="ACHJ12_004725"
     CDS             1835..2710
                     /locus_tag="ACHJ12_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992394.1"
                     /translation="MKQIILPLAILSTLITANYSYARNTQNLIIGSGGNIYKVEDFLP
                     EKNKWTFSSGLSIYENNKSSAFPQYTLNLTSIGSFFLDNSVSVVSTRTKQLNGYASFK
                     YGVTDNLSIRGTWFSKYTRKDTTDSQLNLKSETTGNADNYNIGVSYRLDNFYPLTVLS
                     AGTGFKAGNINSYTISSSFSWVLDPVVLTLYPSYIDGISGKKDDIKYRYFSAAGILSI
                     ALNNDVDVKFGASKDIYTTKKRNPHTPSWDTSGSLITGVTFNNWKNFVVDFTVDHELG
                     GMDANTFSLDFSWRA"
     gene            2712..3323
                     /locus_tag="ACHJ12_004726"
     CDS             2712..3323
                     /locus_tag="ACHJ12_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992395.1"
                     /translation="MKIPVLCTLIITAMLTNTTQAKTSLSYKIDDLEYQPHQIMTDDE
                     MARTTGKAIPVVLAPLLWGAAQGMTIGLSAYAATLAVSGKEFKTRDAVIAAAGGAFSG
                     GVTGATTVTKFMLLEAGGMFFAGLAEENLLGQRKHKAGPAIDAVPTQQDLNKVLNDKN
                     WADKHFNSIPGYSNGRYKQVFEKAIENPASWKKEMTNKCTQCH"
     gene            4125..4781
                     /locus_tag="ACHJ12_004727"
     CDS             4125..4781
                     /locus_tag="ACHJ12_004727"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFO4992396.1"
                     /translation="MLHKIFTLSTKIKTTIITICALSYNLTAQASPSDRPRDRVNKTM
                     IELQTNGVERQLLDNSCGISSLSYILNKYFKKNTSEKNIFLLIGLKPDYSLLDLASTS
                     RTLGISTIGLKLSINDLKKVSSPTILKTKTNGGHFVVYSGYRNGWFQVIDPAKGKLNY
                     YEHELSKIFIQPNKDYGMALIFLSKNKNRFVDETLYNNNTNRLFLERHQIISPVNISF
                     "
     gene            complement(4783..5313)
                     /locus_tag="ACHJ12_004728"
     CDS             complement(4783..5313)
                     /locus_tag="ACHJ12_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992397.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LENKQIMDMKSRKRDK"
     gene            complement(5316..6242)
                     /locus_tag="ACHJ12_004729"
     CDS             complement(5316..6242)
                     /locus_tag="ACHJ12_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO4992398.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGLDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            6420..>6643
                     /locus_tag="ACHJ12_004730"
     CDS             6420..>6643
                     /locus_tag="ACHJ12_004730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992399.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTVRQRQIIDLLDNEDGLTEQER
                     KLLHVLATAEKNDLQAQENERKQTIQKRIEG"
BASE COUNT         1936 a         1578 c         1350 g         1779 t
ORIGIN      
        1 aaaaaaacaa cgacgggaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga actatactat aaaaaagaca tgatattaca
      121 gaaacttaaa tcgataaaat acgatctgga cacataccca aacccagacg cgtaagtatt
      181 cccattcaca ttctggttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcgcaatc atgcggaaag aaacgccatt gctgggataa
      301 acgacgaaaa acctacgact caaaaggacg caaaaaaggc gctgttatct gatataggtt
      361 ttatttggtc tactctacaa gacctcgttt tttcagttgg taataccagg cgcggctaat
      421 tccaagagtc tcccacggtt tcaacgttcg cgcagatgtg gcaacagcac cacgcttaga
      481 tttagcacct ccaattttcc cgcccttgcg accacgtgca gcctgaattt ctggcgtgtg
      541 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
      601 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
      661 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
      721 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
      781 cccgtaatgt ttatcgatgc tacgacgctc tgaggcgctc aaatcgagat aatcagccag
      841 ttcatcgagg gtataggctt cctcgcgcca ctccatcacc agccattcca ggtggaacgg
      901 atttttgcaa atcaggccgc tgtagttcac atccgcacaa agtttctcac acagcgaacg
      961 ttcaaccgct gcggcatatt tcagcgcctt aaccgaggca tcaggcgcgg ttctcacggc
     1021 gatgttgagt gcgtagagca ggtgagcgtg tccgttcaca ggatttttaa cggtgatgtt
     1081 cggcgcggga gcgttccggt cgctccagtc aatagcagcc ccctcgcggt caacgtcaaa
     1141 aaccagccag aactgtgcat gaggctggtt ctgctggata taccgtgcaa gaagcgcgcg
     1201 ccctttgccg gagatacgaa ggccgaaagc gagatcatcc gtgtgatagg gacggtgggg
     1261 taaattttct tcgaagtatt gaagcgcggc gctcatgtgt tacgattctc tccgggttat
     1321 gcgacctaca cgccctctgt atttcatgtt gaatcttgcc ggatctggac atgatttcag
     1381 gggcgtttgt ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc
     1441 gaaaacccga tccatttacc ataactcccg ttgcacgtac cccttaaagc gccctcaatc
     1501 gctttctggc gcaaaaaacg caaaaagaca agaaaacaca aggctaaacc agaaaaacgc
     1561 gccagaatcg tttttagagg gctgcataac tatgcacgaa agcgacccgc gatcggtttc
     1621 gtgtgttctg aacaccggac ggggcaaaat gacacgtttt ctccccctgg atggagcgtt
     1681 ctatacagag atttttgttc ccctgtctct gtcgtgtaac cacttattga gacggagatc
     1741 cattattcat gttttttaat tgacaactat ttggcatatg gcagaatcaa aaaacagata
     1801 ataattatca ttatcattgg cggtgtaata gatcatgaag caaatcatat tacctttggc
     1861 catattatcc acgctcatta ctgctaatta ttcttacgca agaaacactc aaaacctgat
     1921 aataggctct ggtgggaaca tatataaagt tgaagacttt ttacctgaga aaaataaatg
     1981 gacgttcagt tccggcctgt caatttatga aaataataaa tccagcgcat ttccacaata
     2041 tacgctaaat ctcacatcta ttggatcttt ctttctggac aattccgtat cagtagttag
     2101 taccagaaca aaacaactga acggttatgc atctttcaag tacggcgtca ctgataatct
     2161 atccatcagg gggacgtggt tcagtaagta caccagaaaa gacacaactg attctcagct
     2221 taacctgaaa tcagaaacca caggcaatgc agataactat aatataggtg tttcatatcg
     2281 tcttgataat ttctatccac tgacagtatt aagtgcaggg acaggattta aagcgggaaa
     2341 catcaacagt tacactatat catcctcatt cagttgggtt ctggatcctg tagttctcac
     2401 actctatccg tcctatatcg acggaatatc cggaaagaaa gacgacataa agtaccgtta
     2461 tttctcagca gcaggtatcc tcagcattgc attaaataat gatgtggatg ttaaatttgg
     2521 agcatccaaa gatatctata ccactaaaaa aagaaacccc cacacaccat catgggatac
     2581 ttcaggttcg ttaatcaccg gagtgacatt taacaactgg aagaattttg ttgtggattt
     2641 tacagttgat catgaacttg gtggtatgga tgcgaatacg ttttccctcg atttttcgtg
     2701 gagagcatga aatgaagata ccagttttat gtactcttat aataacagcc atgctgacga
     2761 acaccacaca ggcaaaaaca tcattatcct ataagattga tgacctggaa taccaaccgc
     2821 atcagattat gacggatgat gaaatggcca gaacaacagg aaaagcaatt cctgttgttc
     2881 tggctccact actttggggg gcagctcagg gcatgacgat tggcttaagc gcttatgctg
     2941 ctacactggc tgtttcagga aaagaattta aaaccagaga tgctgtgatt gctgccgctg
     3001 gaggtgcttt tagtggagga gttactggag caactacagt tacaaaattt atgctactag
     3061 aagctggggg gatgttcttt gcaggactgg ctgaagaaaa tcttcttgga cagaggaagc
     3121 ataaagcagg gccagctatc gatgctgttc ctacgcaaca agatttaaac aaggttctga
     3181 atgacaaaaa ctgggctgat aaacatttca attcaatacc cggatattca aatgggagat
     3241 ataaacaggt atttgaaaag gcaattgaaa atccagcatc atggaaaaaa gaaatgacga
     3301 ataaatgtac tcagtgccac taacctgact agaattagtc ttaatacagg agaatttatt
     3361 ttgaaatatg attttatcaa tggcagttct attttaatta tagtctggat gactatagct
     3421 ataataatca gagtcacaaa ctggaaattc aacaaaaaaa cagacccagc tataagctta
     3481 ttcttagctt tccctgcatc tatagttatt ctgttagttt atattttttt cttcgataag
     3541 tgatatgttt tcatgattaa tcaagtttct gttcaaatgc tcctgctggc ccagcatgga
     3601 aaaagaaatt acgaataaat gcacccagtg ccattaacat ctatctacac aacaatagag
     3661 gaaaacaatc atgatatatc aaatagttac aagcagctcc attcttttta ttgtttgggg
     3721 gattacatct ataataatta gaataaagag ttacttttct aagaagaaaa cagaccttaa
     3781 tataagcttt tttatagcct tccctatttc tataataatc cttttatttt gccgaggtat
     3841 attataaata taacataata catctatatg gggtgtgcat atacccacat aaaaacacat
     3901 actcacgggg agaaactatg ttttccaatt ctcactacat gtacataatt attcttgtac
     3961 tatggtttag tattctcaag tacttacaga gaaagaatag catattacat cctttatttt
     4021 cactaatact gacaccacta accattttaa tcattagact tatgatagat ttaatatata
     4081 accaataaat aaattataaa caaaataaaa atagggaaca ttatatgctt cataaaattt
     4141 tcacattaag cacaaagata aaaacaacca tcattacaat atgtgcatta tcatataatc
     4201 ttacagcgca agcttccccg tcagacagac ctcgtgatcg agttaataag actatgatag
     4261 aactacaaac taacggagta gaacggcaac ttttagataa ttcttgtggc atatcctctc
     4321 tgtcttacat acttaacaag tattttaaaa agaacactag tgagaaaaat atttttctgc
     4381 ttataggtct gaaaccagat tattctctgc tagatctggc atcaacatcc cgaacacttg
     4441 gaatatcaac tattggacta aaactatcta ttaatgacct gaaaaaagtg agttcaccaa
     4501 caatactaaa aaccaagact aatggcggac actttgttgt ttattctggc taccgaaacg
     4561 gatggtttca ggtcatcgat cccgccaagg ggaaactcaa ctattatgaa catgaactca
     4621 gtaaaatatt catacaacca aacaaagact atggaatggc gcttatcttt ctatctaaga
     4681 ataaaaacag atttgtagat gagacacttt acaataacaa tacaaaccgt cttttcctag
     4741 agagacatca gatcatcagc cctgtaaaca tatcattcta attcatttgt cacgcttcct
     4801 actcttcatg tccatgatct gtttgttctc cagggtcaca actttctgat acaccgccgc
     4861 tgtggcgttg tagttcctgt cgagtattgt tttcattccc catccgagca cacagaacat
     4921 aaccagaaaa gccactgaca ccccacccag ccatttccag ctataccctt taagggtgtt
     4981 gtattcctgt acgatattgc tgtgaattgt cgcaatatcc ctgttcaggg catccgcgtt
     5041 tgccttcatg tttgcgactg actcttccag ccgctgaaaa tcctggctga tgcgctcacc
     5101 tatcagccct gccacatcca gcttctgcac ctcatttcgg acttcatccc tgacctgttt
     5161 tgccacgccg tcagaaatat cccgatatcc gcgaagtgtt tcccttgcca cagccagaga
     5221 atcagcaaca gctttctgtt gctccattgc ggtatgttgc agatgctcca ccgataacag
     5281 gagcgtgagt aatttttctt ctgtgctggc catgttcaga atcccataga aaatccacgt
     5341 gacggtgatt taacccgttc aggttcagtt cggtcctgtc tgctcactct gtctctcatg
     5401 tcaaggccat gctcccgcgc aacatcatgt tgaagccttc tgaccagttc atgttgctta
     5461 accggagtgt ccagccattc cctgatgtca tgcccgaccg ttcgcgcctg ttcaagatgg
     5521 cgggaatagt ccggttcaga ccgtttttgc atgatgtcat gacgctgggc atcatcaagc
     5581 cgtccggcag cttcaaaacc cagatgcact tcgggacgtc gttcaatgcc ctgttctttc
     5641 agcgtccggc agtcaatgaa gcccctttca aagccatgct ttctcatatg ttcgttgtgc
     5701 ttcacctccc agcgttttcg caattcccgc aactgctctt tttgctgagt gggagtcagg
     5761 cttccggaat cctttttcgc acctccccgc tcgggatacc tggcgttata gcgccggaaa
     5821 tactgttccg gtgtacgttc aatgccatca ttcacacgct gactcatcat gatatgcgca
     5881 tgaggctgtt cgccgccgtc aatgctcgct ttcggattgt gaatggcaaa actccaggcg
     5941 tgccgttcgc cggcttcctg acgtacaaaa tcctgcacca gttcaaggcg ctgttccgct
     6001 gtcagttcac ggggtaatgc aatttcaagt tccctatagg tcgaaccgtt cgcacgttca
     6061 aactggtcag ccgcctgcca gaaatgggaa ggattatccc tggcccattc cggcatgttt
     6121 ccgtgtgcgg aatattccaa atcctgacgg tcgcggtatt tctctttgcg ctcgatgtaa
     6181 tcggcgtggt ttgcagcctg ccccttgcca ccaaatttaa cgctgaggtg ataggttgcc
     6241 acggtttcag ccctccgcag gagcgcactg tttctcgacg aaggagagaa acatataagt
     6301 gcgcttcctt ctaattttta gaattagcac ggaagatacc cccctgcacc caccgaccgc
     6361 aagcgatata ctgcacgaca acaaaacatc gcaaaaaaac accagggagt accacgaaca
     6421 tgcgcatcag caacatcgaa tggctgaaga aacggatcgg attcatcagg aagctcggag
     6481 aacagaccgt gcgacagaga cagattattg acctgctcga caacgaagac ggactcacag
     6541 aacaggaacg caaacttctc cacgtgctgg caacagcgga aaaaaacgac ttacaggcac
     6601 aggagaacga gcgaaaacag acgatccaga aacgaatcga ggg
//
LOCUS       DAZFFI010000188         6630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFFI010000188 DAZFFI010000000
VERSION     DAZFFI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.190"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            115..843
                     /gene="gadW"
                     /locus_tag="ACHJ12_004731"
     CDS             115..843
                     /gene="gadW"
                     /locus_tag="ACHJ12_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4992400.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHJ12_004732"
     CDS             complement(1206..4319)
                     /gene="mdtF"
                     /locus_tag="ACHJ12_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4992401.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHJ12_004733"
     CDS             complement(4344..5453)
                     /gene="mdtE"
                     /locus_tag="ACHJ12_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4992402.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5561..5839
                     /locus_tag="ACHJ12_004734"
     CDS             5561..5839
                     /locus_tag="ACHJ12_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992403.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5840..6367)
                     /gene="gadE"
                     /locus_tag="ACHJ12_004735"
     CDS             complement(5840..6367)
                     /gene="gadE"
                     /locus_tag="ACHJ12_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4992404.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1669 a         1611 c         1612 g         1738 t
ORIGIN      
        1 gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt ctggcaacat
       61 ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat aatcatgact
      121 catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga acagcataaa
      181 atatcgctgc ataacgagag tatcgtgctg ctggagaaaa atttggcaga cgattttgcg
      241 ttttgttcac cggatacgcg acgactggat atcgatgagc tgacagtttg ccattactta
      301 caaaatattc gtcagctacc acgcaattta gggttacata gcaaagaccg tctattaatt
      361 aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagcttcaa tgaatccggg
      421 gtaaattcac cgatactgag caatatgctc tacctttcct gtttatcgat gttttctcat
      481 aagaaagaac tgatcccctt acttttcaat agcatcagca ctgtttcagg aaaagttgaa
      541 cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc ggaaagaatg
      601 tataccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg tttcagtaaa
      661 atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg tcaaattcct
      721 ctgcatacta ttgcagaaaa atgtggctat agcagtacgt cgtactttat aaacacattt
      781 cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg taccttttcc
      841 tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg tgaaactctt
      901 ccctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc aggcggtata
      961 ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctgac aattcatacg
     1021 attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg ctggagtggt
     1081 ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg gttgttgagt
     1141 ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt actctcattt
     1201 acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa aaagaccgga
     1261 acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc ggtacctacc
     1321 gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac gccgagaata
     1381 aaggccagag aggtcatcag gattgggcgt aaacgcatcc gcgccgcttc gatgattgcc
     1441 tctatcggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac aatcaggatg
     1501 gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta gacgtcatta
     1561 cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg aacaaccaac
     1621 atcaccgaga acggaattga ccagctctca tagagtgcgg cgagggcgag gaacaccacg
     1681 accagtgaaa tggcatacag tgcaggagcc tggtttgagg ataacgcttc ctgatacgac
     1741 agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc tgccataaat
     1801 ttcatagcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc cattgacggg
     1861 atgccgttgt agcgttccaa tcgcggtgaa ccataggtcc attcggtaga cgagtaggca
     1921 gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat gttatccggc
     1981 aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc ctggttgagg
     2041 aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat atcagacagc
     2101 gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat cggcgtatct
     2161 tccaggccgt tcgggcgtac cccggtgacc tggtcaggcg attgcgctgc cagtgataac
     2221 agctcgtttc gtgcctgggt tagtttttcg tgccccaggt taccgttgtc cagcagttcc
     2281 atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa cgggaagacg
     2341 acggctttat tgatactgct taacgcaatc attgcccgct gaatgatcgc ggtaaccgag
     2401 ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag gccgttgttt
     2461 tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactgaac attatctttc
     2521 tcttttgtaa gataataatc cgtcacctgt tgcagcactt tcgtggtgtt aaccatggtg
     2581 gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc tggtaagaaa
     2641 gaggtcggtg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag gtagaccacc
     2701 atgtagcgac cggtacaacg caacagcgag cgggtgctat cggtatagtg ctgagttgat
     2761 ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc gccttccggc
     2821 gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgccac aaatactgaa
     2881 agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc ggttgcgccg
     2941 ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cagcaatacc gaccagcgca
     3001 cgttggatct gccccatcga tttatgcgtc gcttccttcg gtggtagctt atcttccgca
     3061 atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa ccctatcgcc
     3121 agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga caagatcgca
     3181 aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc acggaaattc
     3241 tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag tgttttaaaa
     3301 acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac cgtcttcaga
     3361 cttgcgggga aataggctga taagcggttc agctcctctt tgactgcccg cgaggtatcc
     3421 agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt gccgttatag
     3481 cgcgccacgg tggaataatc ttccgctcca agttcgacgc gggcgacatc acgcagcagc
     3541 acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc cggcgtttgc
     3601 agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg tggcatgcca
     3661 cccagttgac caccggaaat ctggttgttt tgcaccttaa tctgggaaat aacatcggaa
     3721 ggtaccagat tgtatttatt gagtttttgc gggtccagcc agatacgcat ggcgtactcg
     3781 gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc tttgatatta
     3841 gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga aataaacgcc
     3901 gctaccatca gaatattact gctcgactta tcaacgctaa tcccctgctg ctgcaccgct
     3961 tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg tgcgatatca
     4021 ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc atcactggtt
     4081 gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac ctgagtgacc
     4141 gagtcttcta ccgtttgcgc atcggcacct ggataggtag cgctgacggt aatggtcggt
     4201 ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc acctgcaagc
     4261 atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata gttagccatg
     4321 aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt ttcctggctg
     4381 gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga aacgatcacc
     4441 cgatcaccag cctgcaagcc agaggtgaca acccactggt cgccgatggc tttgctggct
     4501 tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt tgctttaccc
     4561 tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg gctaccttca
     4621 tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt ggggaaaatc
     4681 gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa tttcagcgtg
     4741 ccggtctggc tgtagcgttt accattttcc agattgagct gtactggcgt actgccctga
     4801 acctgtttga tttgcccact ggcgacctct tctttcatgc gtaagaaatc ctgcaccgac
     4861 tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga atctgcctga
     4921 ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc
     4981 gaggtgacat tcgcgtattg cagattgatc gtcgcctgtt caacagccgc tttggcgacg
     5041 gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc ctgacggcta
     5101 acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc attgctggcg
     5161 gtagagagcg ctttcgccag cgagcctttg gcggagttta gctcggcctg taaaggtgca
     5221 ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat aaagttgcgt
     5281 ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg aacggttcta
     5341 ccgggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac accgacctca
     5401 ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg
     5461 cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc tgaaaattct
     5521 tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt tacagaccag
     5581 cggcatagtg gggttgtcag ccagtaaggt gggatacagg cacagtgatc gacatggtga
     5641 ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca ttcgggcaac
     5701 gaagggttct actggtggat acacatacca ggggaataaa gtcgttttag cctctccttt
     5761 agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca gccatcaatt
     5821 tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg atgtcgctca
     5881 tacgtcttag tttcagcttg tacataatgt tgaatttctg aatctttaat gtttgttgcg
     5941 aaatattgag cgtcgacgtg atattgcttt catttttatt tttcagaatc agttgcataa
     6001 ttttgaactg attatgtgaa aaacataatt gtgaattctt atggggcaag tgtttaccat
     6061 aagtaatctc aagaagattt ttgatacttt ctttgcggct aacaaaataa ataggcgctt
     6121 tagcttttag tttactgatg tgataaggtg ccaaaacaat aattctctcg gcatctaatt
     6181 tctccagaaa tttaatcgct tcttcatcaa ggatatgatt gtgataggta tcaatgataa
     6241 ccttgaaggg tttattgcct acttttttga tctctgacag ggaattgatt tttatcattt
     6301 cgtgattatc tttcaactgc caaaagccct gtaaaagaaa agaatctttc gtcatgagaa
     6361 aaatcataac ttgctcctta gccgttatcg tttcgaacat gtcatccttt tcattattta
     6421 catccttgtc cgaatcgttg ttcaatatag taaacgccta taagaacttt gcccttcgtg
     6481 aactaatacg tctattaaca ctaatcaata cttcagattt atccatcacg cactccatat
     6541 attgatccta agtaagattc ctggttgtta tcagtttgta aacagtaaac aaaaagttaa
     6601 cgtttgcgga ttactgccca agaataagtt
//
LOCUS       DAZFFI010000189         6391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFFI010000189 DAZFFI010000000
VERSION     DAZFFI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.191"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            274..1050
                     /gene="exuR"
                     /locus_tag="ACHJ12_004736"
     CDS             274..1050
                     /gene="exuR"
                     /locus_tag="ACHJ12_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4992405.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1290..1373
                     /gene="yqiM"
                     /locus_tag="ACHJ12_004737"
     CDS             1290..1373
                     /gene="yqiM"
                     /locus_tag="ACHJ12_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4992406.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1395..2057
                     /gene="yqjA"
                     /locus_tag="ACHJ12_004738"
     CDS             1395..2057
                     /gene="yqjA"
                     /locus_tag="ACHJ12_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4992407.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2061..2444
                     /gene="mzrA"
                     /locus_tag="ACHJ12_004739"
     CDS             2061..2444
                     /gene="mzrA"
                     /locus_tag="ACHJ12_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4992408.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2591..2959
                     /gene="yqjC"
                     /locus_tag="ACHJ12_004740"
     CDS             2591..2959
                     /gene="yqjC"
                     /locus_tag="ACHJ12_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4992409.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2997..3302
                     /gene="yqjD"
                     /locus_tag="ACHJ12_004741"
     CDS             2997..3302
                     /gene="yqjD"
                     /locus_tag="ACHJ12_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4992410.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3305..3709
                     /gene="yqjE"
                     /locus_tag="ACHJ12_004742"
     CDS             3305..3709
                     /gene="yqjE"
                     /locus_tag="ACHJ12_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4992411.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3699..3998
                     /gene="yqjK"
                     /locus_tag="ACHJ12_004743"
     CDS             3699..3998
                     /gene="yqjK"
                     /locus_tag="ACHJ12_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4992412.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4184..4576
                     /gene="yqjF"
                     /locus_tag="ACHJ12_004744"
     CDS             4184..4576
                     /gene="yqjF"
                     /locus_tag="ACHJ12_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4992413.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4646..5632
                     /gene="yqjG"
                     /locus_tag="ACHJ12_004745"
     CDS             4646..5632
                     /gene="yqjG"
                     /locus_tag="ACHJ12_004745"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4992414.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5925..6290
                     /gene="yhaH"
                     /locus_tag="ACHJ12_004746"
     CDS             5925..6290
                     /gene="yhaH"
                     /locus_tag="ACHJ12_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4992415.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1627 a         1540 c         1585 g         1639 t
ORIGIN      
        1 gtttccaaaa agttggtgta ttagagatgt agatttattg taagtcaata taaaaaagca
       61 tttttagtta atattaaatt taaaggctgg gaattaactt actgaaattt agtagctttc
      121 acaatgacga tacttatttt ttaggaattt ataaatattc acaatattaa ttcaatacat
      181 taattcaatc accaaattta tatatgacca atggcatacc catgaataaa gtggtatgat
      241 aacttcgtcc gcattccact ccgggagacg catatggaaa tcactgaacc acgccgtttg
      301 tatcaacaac ttgccgctga cctgaaagag cgcatcgaac agggcgtcta tctggtaggt
      361 gataaactgc ctgcagaacg ctttattgcc gatgaaaaga acgtcagccg tacggtagtt
      421 cgtgaagcca tcatcatgct ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt
      481 attcatgtgg tttccaacca gccgcgccat cagcaggcag ctgacaataa tatggaattc
      541 gccaattacg gtccgtttga gttgcttcag gctcgccagc tcatcgaaag taatattgcc
      601 gaattcgcgg caactcaggt aacgaaacag gacatcatga aactgatggc cattcaggaa
      661 caggcgcgcg gcgaacaatg cttccgtgat tccgagtggg atttacagtt ccacattcag
      721 gtagccctgg cgacgcagaa ctccgccctg gcggccatcg ttgaaaaaat gtggacacaa
      781 cgtagccata acccgtactg gaaaaaactg cacgaacaca ttgattcccg taccgtcgat
      841 aactggtgtg atgaccacga tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct
      901 gctaagctgg caatgtggca gcacctggaa aacaccaaga tcatgttatt caacgaaacc
      961 agcgacgact tcgagttcaa tgccgaccgc tatctgttcg ccgaaaaccc ggtagtgcat
     1021 ctcgatacgg ccaccagtgg cagtaaatga aactcctgtc cgacaggcgc tgcgacgcgc
     1081 ctgtttgtct cgtcggtaag cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc
     1141 accctcccct gcattcagca aaatcagact ccacggacat gtaattttga taacgaacaa
     1201 cgttgacctt tgttacaatt agattcaatt tgaatttatg tttttgaatg ctttcttatc
     1261 tcacgattta acagggaata gttcaggctg tgttgatgta tcaaacccgc agaacatacc
     1321 aaaacagcaa taacattgcg gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc
     1381 accaggaata atgaatggaa cttttgaccc aattgctgca agccctgtgg gcgcaggatt
     1441 ttgaaaccct ggccaatcca tcgatgattg gcatgttgta ttttgtcttg tttgtaattt
     1501 tgttccttga aaacggcttg cttccagcgg cctttttacc gggcgacagt ttactggtat
     1561 tggtcggcgt gttgattgcg aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga
     1621 ccgttgccgc cagcctcggc tgctgggtca gctatattca ggggcgatgg ctgggcaata
     1681 cccgcaccgt acaaaactgg ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc
     1741 atctttttca taaacacggt ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca
     1801 gaacactgct gccgacgatt gccgggttat cagggctgaa taacgcgcgc tttcagtttt
     1861 tcaactggat gagcggtctg ctgtgggtat tgatcctgac aactctgggt tacatgctcg
     1921 gcaaaacgcc ggtattttta aagtacgagg accagctgat gtcatgcctg atgctgctcc
     1981 cggtggtgct gctggtgttt ggcctggcag gttctctggt cgtgttatgg aaaaagaaat
     2041 atggaaatcg ggggtaaggg atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt
     2101 ccggcactgt tttacttctg gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc
     2161 aagagtctac gctggcgatt cgtgccgttc atcaaggcac aacgatgcca gacggttttt
     2221 cgatctggca tcaccttgac gctcatggca ttcctttcaa aagtatcacc cccaaaaacg
     2281 acactctgtt aattacattt gactccagcg accagagcgc cgccgcaaaa gcggtcctcg
     2341 acagaacatt gccccatggc tacatcattg cgcagcagga caataacagt caggctatgc
     2401 agtggctgac ccggttacgg gataactctc atcgcttcgg ataacttcct gttatccgaa
     2461 acatatcact cactttggtg atttcaccgt aactgtctat gattaatgag gcggtggtta
     2521 acactaccgc ttcgttgtct ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga
     2581 aggttcaaga atgaaatacc gcatcgcttt agctgtttct ctctttgctc ttagtgccgg
     2641 tagttatgcc actaccctgt gtcaggaaaa ggagcaaaat atccttaagg agatcagcta
     2701 tgccgaaaaa caccaaaacc agaatcgtat tgacggtctg aataaagccc tgagtgaagt
     2761 ccgggccaac tgttcagata gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca
     2821 gaaagatgag gtggcggaac gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc
     2881 cgataagatt gccaaacgcg aacggaaact ggcagaagcg caggaagagc tgaaaaagct
     2941 ggaagcgcgc gactactaac tcacaatagt cactacttac tcacctggag aaaactatgt
     3001 cgaaagaaca cactacggaa catctgcgtg ctgagttgaa atccctttcc gatacgctgg
     3061 aagaggtgct tagctcatct ggcgagaagt cgaaagaaga gttgagtaag attcgtagca
     3121 aagcggagca ggcactgaaa cagagccgtt atcgcctggg tgaaaccggt gatgccatcg
     3181 ccaaacaaac ccgtgtcgcg gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga
     3241 ccggcgtggg cattggcgct gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt
     3301 aattatggcg gacactcatc acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca
     3361 gcgaattgtt tccatcatgg ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga
     3421 gctggaagag gaaaaagcta atctctttca acttttactg atgctgggcc tgacgatgct
     3481 tttcgctgca tttggtctta tgagcctgat ggtgctaatt atttgggcgg ttgatccgca
     3541 atatcgcctg aatgcgatga ttgccaccac cgtggtgttg ctgctactgg cactgattgg
     3601 cggtatctgg acgttacgta aatcgcgtaa atccaccttg ctgcgccata cacgccatga
     3661 gctggcaaac gatcggcaac tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg
     3721 aacgacgtaa ggcgcaactg cttagccaga tccagcaaca acggctggat ctttccgcca
     3781 gtcgtcgtga atggctggag gcaacaggcg cttacgatcg tcgctggaat atgctgctaa
     3841 gtctgcgctc ctgggcgctg gttggcagta gcgtgatggc gatctggacg attcgccatc
     3901 ctaatatgct ggtccgctgg gccagacgcg gttttggcgt atggagcgcc tggcgtctgg
     3961 ttaaaacgac cctcaagcag caacagcttc gcggttaact tccctctggc cggagcaatt
     4021 ccggccttat ccctcaaatt ttttgaagat ttttgacagt tttccttgct aacaatcatc
     4081 attcaccacg tttatgattc tctccatcga cagcaacgac gctaataccg cgcccttgca
     4141 caaaaaaaca atcagcagcc tgagtggcgc agtggagagt atgatgaaaa aattagaaga
     4201 tgttggtgta ctggtagcgc gcattttaat gccgattctg tttattaccg caggctgggg
     4261 aaaaattact ggctacgcgg gtacccaaca atatatggaa gcaatgggtg tcccgggttt
     4321 tatgctgccg ctggtgattc tgcttgagtt cggtggtggt ctggcaatcc tgttcggttt
     4381 cctgactcgc accacagccc tgtttactgc gggctttacg ctgctgacgg catttttatt
     4441 tcacagcaac tttgctgaag gcgtcaactc gctgatgttc atgaaaaacc tgacaatttc
     4501 tggcggattc ctgctgctgg caattaccgg tccgggcgcg tatagcatcg accgcctgct
     4561 gaataaaaag tggtaagcac gcgctatact taacgataaa aaacgaggag gaagctcctc
     4621 gtttttgcta ttggaggaga gaaaaatggg tcaactgatt gacggcgtct ggcatgacac
     4681 ctggtacgat accaaatcta ccggcggtaa atttcaacgt tcagcttccg catttcgtaa
     4741 ctggctcact gccgatggcg cacctggccc cactggcaca ggcggtttta tcgcagagaa
     4801 agatcgttat catctctatg tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat
     4861 gcgcaaactc aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct
     4921 ggaaaacggc tggacctttg atgacagttt tccgggagca accggagata cgctctatca
     4981 acatgaattt ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac
     5041 tgttcccgtg ctgtgggaca aaaagaacca caccatcgtc agcaacgaat cagcagaaat
     5101 catccgcatg ttcaataccg cgtttgatgc gctgggcgca aaagcgggtg attactaccc
     5161 accagccctg caaacgaaaa ttgacgaact taacggctgg atttatgaca ccgttaacaa
     5221 cggcgtgtat aaagctggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa
     5281 agtgtttgaa tcgctggcgc gactggagca gattttaggc cagcatcgtt acctgaccgg
     5341 caaccagcta accgaagccg atattcgcct gtggactacg ctggtgcgtt ttgatccagt
     5401 gtatgtgacc cacttcaagt gtgataagca ccgcatcagc gattacctga atctgtatgg
     5461 cttcctgcgc gatatctacc agatgccggg gatcgccgaa actgtcaatt tcgatcacat
     5521 ccgtaatcat tacttccgca gccataagac catcaaccct acggggatta tttcaattgg
     5581 tccgtggcag gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc
     5641 accttacgag gtgcctctga taattagcta agtgcatgtt gttcatgtcg gatgcggcgt
     5701 gaacgcctta tcaaacctac aaaacattta tattcaatat gttgcaatga ccgcgcaggc
     5761 cagataagcg tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcgccttcac
     5821 agcactttat taattccttg aacgaatatt tactgccatt tcattcacgt ctattcttaa
     5881 tttgctgctt taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga
     5941 aagtactaaa aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt
     6001 ttattctggt caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct
     6061 ggcaacgcgc tggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac
     6121 cgtggtgggc agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac
     6181 tgttgttctt aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg
     6241 gtacgccagg cgaaaaccgc tttggaccag atccgaaact ggaaccttaa aacaaatctg
     6301 ctcgccggat gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact
     6361 aatgtttatt taaaatattt caatgtcgtt a
//
LOCUS       DAZFFI010000190         6375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFFI010000190 DAZFFI010000000
VERSION     DAZFFI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.192"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..383
                     /gene="ytcA"
                     /locus_tag="ACHJ12_004747"
     CDS             108..383
                     /gene="ytcA"
                     /locus_tag="ACHJ12_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4992416.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            402..1433
                     /gene="mdtN"
                     /locus_tag="ACHJ12_004748"
     CDS             402..1433
                     /gene="mdtN"
                     /locus_tag="ACHJ12_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4992417.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1433..3484
                     /gene="mdtO"
                     /locus_tag="ACHJ12_004749"
     CDS             1433..3484
                     /gene="mdtO"
                     /locus_tag="ACHJ12_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO4992418.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            3481..4563
                     /gene="mdtP"
                     /locus_tag="ACHJ12_004750"
                     /pseudo
     CDS             3481..4563
                     /gene="mdtP"
                     /locus_tag="ACHJ12_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            4760..6025
                     /locus_tag="ACHJ12_004751"
     CDS             4760..6025
                     /locus_tag="ACHJ12_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4992419.1"
                     /translation="MTKKTKSLSPAAIKSLQRKASNEKKDLFITDGEGLELVIKHKSA
                     SCLWRYRYYKPHTKKRAMLSIGAYPEISIAGARKIKDEYRALLSGGIDPKEHREKQRL
                     SEVMKRENTFRHVATLWFELKKGQGLAEGTLRDTWRSLERNVFPSVGNRPVADLIASN
                     FIEALKPLAAQGKLDTVRRTAQRINEVMYYAVNTGMITANAASQINKAFNAPSAKNMP
                     AIRPEELPDFMQKLATARVERQTRLVIEWQLLTICRPGEAAGARWCEIDFEAKEWRIP
                     EHRLKQRREHVIPLSKQALAILDVMKPISGHREFIFPHNSDPQRPMCTQTANAAIKRM
                     GFGRRLVAHGLRSIASTALNEQGFDSDVIEAALSHIDKNEVRRAYNRSTYLEQRRPLM
                     AWWGNFVEAAASGSVSATGGYRGLKMVNG"
     gene            6030..>6375
                     /locus_tag="ACHJ12_004752"
     CDS             6030..>6375
                     /locus_tag="ACHJ12_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992420.1"
                     /translation="MTNKKINLPERDYYPLDKAARRLGCDVDDLIHFIATGKMCFWYR
                     HHIDEVLTLHNYKNAFNSVSASDYYYENDEGVFIRATDILKLLESSSFGEKIITDEEQ
                     LTEKIFFNCCGGY"
BASE COUNT         1590 a         1647 c         1664 g         1474 t
ORIGIN      
        1 ctgaataaag ctatttttat tcatcgagaa aatagccata ctgacgcctt caatggatat
       61 tgacattcac tacagatatt gttcgcagca gacaggatgt atttaatatg ccaacagttc
      121 tctctcgcat ggcgatgcaa ctcaaaaaaa cagcctggat aattcccgtc ttcatggttt
      181 cgggatgctc attatctccg gcaatcccgg tgatcggtgc ttattatccc agctggtttt
      241 tctgcgccat tgccagcctt attttgatgc tcatcacgag gcgagttatt cagcgggcaa
      301 atatcaatct ggcatttgtc ggaattattt ataccgccct ttttgctctc tacgccatgc
      361 tgttctggct ggcatttttc taatctattt gagacgcgct catggaaagt acgccgaaaa
      421 aagctcctcg cagtaaattc cctgctctgt tagtggttgc attggcgctg gttgcccttg
      481 ttttcgttat ctggcgcgta gacagtgcgc catcaactaa tgacgcttac gcgtcagcag
      541 ataccattga tgtggtgccg gaagtcagcg gccgcattgt agaactggcg gtcaccgaca
      601 accaggcagt caaacagggc gatttgctgt tccgcattga cccgcgcccg ttcgaagcca
      661 atctggcgaa agctgaagcc tccctcgcgg cgctggataa gcaaattatg ctcacccagc
      721 gtagcgttga tgcgcaacag tttggtgccg actcgattaa tgccacggta gaaaaagccc
      781 gtgccgccgc gaaacaggcc acagatacat tacgccgcac cgagccatta ctgaaagagg
      841 gttttgtctc agcggaagac gttgaccgtg caagaactgc gcagcgcgcc gcagaagccg
      901 atcttaatgc cgtattgtta caggcgcagt cagccgccag cgccgtcagc ggcgtcgatg
      961 cgctggtggc gcaacgtgcg gcggtcgaag cggatattgc cctgaccaaa cttcatctgg
     1021 aaatggcgac cgttcgcgcg ccgtttgatg gccgggtcat ttccctcaaa acctccgtcg
     1081 ggcaatttgc ttctgccatg cgccctattt ttaccctaat cgacactcgt cactggtacg
     1141 tgatcgccaa cttccgcgaa accgatctga aaaatattcg ctcaggtaca cccgcaacga
     1201 ttcgcctgat gagtgacagc ggcaaaacct tcgagggtaa agtggattcg attggctacg
     1261 gcgtgctacc ggatgacggc ggcctggtgc tgggcggcct gccgaaagtt tctcgctcta
     1321 ttaactgggt ccgcgttgcc cagcgttttc cggtcaaaat catggtcgat aaacctgacc
     1381 cggaaatgtt ccgcatcggc gcttcggcag tcgctaatct tgagccgcaa taatgagcgc
     1441 gctcaactcc ctgccattac cggtggtcag gctgctggcg ttctttcatg aagagttaag
     1501 cgagcggcga ccaggccgcg tgccgcagat cgtgcaactc tgggtaggct gcctgctggt
     1561 gattctgatc tcgatgactt ttgagatccc ttttgtggcg ttatcgctgg cagtgctgtt
     1621 ttacggtatt cagtcgaacg cgttttacac caaatttgtc gcgatcttgt ttgtggttgc
     1681 cacggtgctg gagatcggca gcctgttttt gatctacaaa tggtcatacg gcgaaccgtt
     1741 gatccgattg atcatcgccg ggccgatcct gatgggctgt atgtttttga tgcgcaccca
     1801 tcggctggga ctggtctttt tcgctgtcgc cattgtcgcc atttacgggc aaaccttccc
     1861 cgccatgctc gactatccgg aagtggtcgt gcgcttaacg ctgtggtgta tcgttgttgg
     1921 cctctatcca accttgctga tgacgttaat cggcgtgctg tggtttccca gtcgtgccat
     1981 tacgcaaatg catcaagcgc ttaatgatcg gcttgatgat gccattagtc acctgacgga
     2041 cagcctcgca ccgctacccg aaacgcggat tgaaagagag gcgctggcgc tgcaaaaact
     2101 gaatgtcttt tgtctcgcgg acgatgccaa ctggcgaact caaagcgcat ggtggcaaag
     2161 ctgcgtggca acggtaacct acatttactc gacgctgaat cgctacgatc ccacctcttt
     2221 tgctgattct caggcaatta ttgaatttcg gcaaaaatta gcctcagaaa tcaataagct
     2281 gcagcatgcc gttgctgaag gtcagtgctg gcaaagcgac tggcggatca ctgaaagtga
     2341 agcgatggcg gcacgggaat gtaacctgga gaatatctgc cagacgttgt tacaactggg
     2401 ccagatggac ccgaatacgc cgccaacgcc cgccgccaaa ccgccatcaa tggtcgccga
     2461 tgcttttacc aatccagact atatgcgcta cgcggtaaaa acactgctcg cctgtttgat
     2521 ctgttacacc ttctacagcg gcgtggactg ggaaggcatt cacacctgta tgctgacatg
     2581 cgtgatcgtc gctaacccaa atgtcggttc gtcgtaccag aagatggtgc tgcgttttgg
     2641 cggggccttt tgcggcgcga ttctggcgct gttattcacg ctactggtca tgccctggct
     2701 ggacaatatt gtcgaattgc tgtttgtgct ggcaccgatt ttcctgttgg gcgcatggat
     2761 tgccaccagc tctgaacgct cttcttatat cggcacacag atggtggtca ccttcgcgct
     2821 cgccacgctc gaaaacgttt ttggcccggt gtacgacctg gtggaaattc gcgatcgcgc
     2881 catgggtatc atcattggta ccgtggtgtc cgcggtgatt tacacctttg tctggcctga
     2941 aagtgaagcg cgcacactgc cgcaaaaact ggctggcacg ctgggtatgt taagtaaagt
     3001 aatgcggatc ccacgccagc aggaagtcac ggctctgcgc acttatctgc aaattcgtat
     3061 cggtctgcat gcggcgttta atgcctgtga agagatgtgc caacgcgtgg cgctggagcg
     3121 tcaactggac agcgaagaac gcgccttact gattgaacgt tcgcaaacgg ttattcgtca
     3181 gggccgcgat attcttcacg cctgggatgc cacctggaac tcggcgcagg cgctggataa
     3241 cgcactacag ccggacagag cagctcagtt tgccgacgcc ctggagaaat acgctgccgg
     3301 tctggcaacc gcactcagcc gttctcctca aataacgctt gaagagacac ccgcctcgca
     3361 ggccatcctg cccaccttat taaaacagga gcaacacgtc tgccagcttt tcgcccgctt
     3421 gccagactgg acagccccgg cattaacgcc cgccacggaa caggcacaag gagccacgca
     3481 atgatcaatc gtcaactttc acgtctgctg ttgtgcagca ttctcggcag cacgacgctg
     3541 atttccggct gtgccctggt acgtaaggat tctgcacctc atcaacagct caaaccggaa
     3601 caaatcaaac tggccgacga tattcatctt gccagctccg gctggccgca ggcgcagtgg
     3661 tggaaacaac tcaatgaccc gcagctggat gcgctgatcc aacggacgct aagtggttca
     3721 cacaccctcg ccgaagcgaa actgcgggaa gaaaaagcgc agtcccaggc cgatttgtta
     3781 gatgccggtt cacaattaca ggtcgcagcg ttagggatgc tcaaccgcca acgcgtctcg
     3841 gcgaacggct ttttaagccc ttatgcgatg gatgcgcccg cactgggtat ggacgggccg
     3901 tactatacgg aagccacagt aggtttgttt gccggactgg atcttgattt gtggggtgtg
     3961 catcgctcag cggttgccgc cgccattggc gcgcataatg ccgcgctggc agaaaccgca
     4021 gcagtagagc tatcgctgac cacgggcgta gcgcagcttt attacagtat gcaggccagc
     4081 tatcagatgc tcgatctgtt agaacaaact cgcgatgtga ttgattacgc ggtgaaagcg
     4141 caccagagta aagtggcgca cggtctggaa gcacaagtgc ctttccacgg cgcgcgggca
     4201 cagattctgg cggtcgataa acaaattgct gccgtcaaag ggcaaatcac cgaaacgcga
     4261 gaatctctgc gtgcattgat tggcgcgggg gccagcgata tgccggagat caaaccggtg
     4321 gcattaccac gagtccagac cggcattccg gcgacactct cttatgagtt gctcgccaga
     4381 cgcccggatc tgcaagccat gcgctggtat gttcaggcgt cattagatca ggtggattcc
     4441 gcgcgggcgc tgttctaccc gagctttgat atcaaagcgt ttttcggcct tgatgccatc
     4501 cacctggata ccttattcaa aaaaaccagt cgccagttca acttcatccc gggccaccaa
     4561 atcgatacaa agtaaagaca agttacggaa agttaaacaa agaaaacccg caccagacaa
     4621 ggaacgcagt ttttttattg ttcagtttgg gtcagtttga tagcgtataa gcaagattaa
     4681 aaaggcatac aaaaaggcat acgcatgaaa aaaacacaga tttgcatatg cctttaacac
     4741 gcttattgga gtttaaccgg tgaccaaaaa aactaagtcc ttatcaccag cagcgataaa
     4801 aagcctccag cgaaaagcca gcaacgaaaa aaaggacctt ttcatcactg acggcgaagg
     4861 gctggaactg gtgattaagc acaaaagcgc ctcttgcttg tggcgttacc gctactacaa
     4921 gccacacaca aaaaaacggg ccatgttaag cattggcgca tatccagaaa taagcattgc
     4981 gggcgcgcga aagataaaag acgaatacag ggcgcttctt tctggtggca ttgacccaaa
     5041 agagcacaga gagaaacagc gccttagtga agtgatgaag agagagaaca ccttcaggca
     5101 tgtagctacg ctttggtttg aacttaaaaa agggcaagga ctcgcagaag gcacactacg
     5161 cgatacatgg cgctcgctgg agcgtaatgt attcccgtca gtcggcaaca gaccagtagc
     5221 agacctgatc gcaagcaact tcatcgaagc attaaaacca ttagccgccc aggggaaatt
     5281 agataccgtc agaagaacgg cgcagcgaat caatgaggtc atgtattacg ccgttaatac
     5341 cggaatgatt accgcgaatg cggcatcaca gataaacaag gcatttaacg cgccgagcgc
     5401 taagaacatg ccagcaatac gccctgaaga actccccgat tttatgcaga agctggcaac
     5461 ggcacgagtg gaacgacaaa cgcggctggt tattgagtgg cagttactga caatttgccg
     5521 accaggtgaa gcagctggcg cgcgctggtg tgagattgat tttgaggcga aggaatggcg
     5581 cataccagag cacagactta aacaacgcag ggaacacgta ataccactct caaagcaggc
     5641 gttagccatt ctggatgtca tgaagccaat aagcgggcat cgtgagttta tatttccaca
     5701 caacagcgac ccgcagcgcc ctatgtgcac acaaacagca aacgcagcaa taaaacgcat
     5761 gggttttggt cggcggctgg ttgctcatgg cttgcgctca atagcaagta ccgcacttaa
     5821 cgagcaaggt tttgactctg acgtaatcga ggcggcattg tcacacatag acaaaaacga
     5881 ggttcgccgc gcgtacaacc gctcaaccta tctcgagcag cgacgcccgt taatggcgtg
     5941 gtgggggaat tttgtagagg cagccgcaag cgggagcgta agcgccacag gtggctatcg
     6001 tgggttaaaa atggttaacg gttaagggca tgacaaacaa aaagataaac cttcctgaaa
     6061 gggattatta cccgttagac aaggcagcca gaaggcttgg gtgtgacgtt gatgatttga
     6121 ttcacttcat cgctacaggg aagatgtgtt tttggtaccg tcatcacata gacgaggttt
     6181 tgacattaca taattataaa aacgcattta atagtgtatc agcatcagat tattattatg
     6241 aaaatgatga aggcgtattc atcagagcaa ccgacatatt aaaattactt gaatcctcat
     6301 cttttggtga aaaaataata accgatgaag aacagctaac ggaaaagata ttttttaatt
     6361 gctgtggcgg ttatt
//
LOCUS       DAZFFI010000191         6331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFFI010000191 DAZFFI010000000
VERSION     DAZFFI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.193"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1040
                     /gene="pfkB"
                     /locus_tag="ACHJ12_004753"
     CDS             111..1040
                     /gene="pfkB"
                     /locus_tag="ACHJ12_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4992421.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1141..1431
                     /gene="ghoS"
                     /locus_tag="ACHJ12_004754"
     CDS             1141..1431
                     /gene="ghoS"
                     /locus_tag="ACHJ12_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4992422.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1537..2397
                     /gene="yniA"
                     /locus_tag="ACHJ12_004755"
                     /pseudo
     CDS             1537..2397
                     /gene="yniA"
                     /locus_tag="ACHJ12_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
     gene            complement(2438..2974)
                     /gene="yniB"
                     /locus_tag="ACHJ12_004756"
     CDS             complement(2438..2974)
                     /gene="yniB"
                     /locus_tag="ACHJ12_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4992423.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3121..3789
                     /gene="hxpB"
                     /locus_tag="ACHJ12_004757"
     CDS             3121..3789
                     /gene="hxpB"
                     /locus_tag="ACHJ12_004757"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4992424.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3952..4542
                     /gene="ydjM"
                     /locus_tag="ACHJ12_004758"
     CDS             3952..4542
                     /gene="ydjM"
                     /locus_tag="ACHJ12_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4992425.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4675..6066
                     /gene="tcyP"
                     /locus_tag="ACHJ12_004759"
     CDS             4675..6066
                     /gene="tcyP"
                     /locus_tag="ACHJ12_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4992426.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6070..>6331)
                     /gene="ydjO"
                     /locus_tag="ACHJ12_004760"
     CDS             complement(6070..>6331)
                     /gene="ydjO"
                     /locus_tag="ACHJ12_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO4992427.1"
                     /translation="PLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQE
                     CIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1557 a         1559 c         1578 g         1637 t
ORIGIN      
        1 tatatattta tctgcaaaat tttaaataaa gctctaataa atcatattgt taatttcttc
       61 actttcggct gattcggtgc cagactgaaa tcagcctata ggaggaaatg atggtacgta
      121 tctatacgtt gacacttgcg ccctctctcg atagcgcaac aattaccccg caaatttatc
      181 ccgaaggaaa actgcgctgt accgcaccgg tgttcgaacc cgggggcggc ggcatcaacg
      241 tcgcccgcgc cattgcccat cttggaggca gtgccacagc gatcttcccg gcgggtggcg
      301 cgaccggcga acacctggtt tctctgttgg cggatgaaaa tgtccccgtc gctactgtag
      361 aagccaaaga ctggacccgg cagaatttac acgtacatgt ggaagcaagc ggtgagcagt
      421 atcgttttgt tatgccaggc gcggcattaa atgaagatga gtttcgccag cttgaagagc
      481 aagttctgga aattgaatcc ggggccatcc tggtcataag cggaagcctg ccgccaggtg
      541 tgaagctgga aaaattaacc caactgattt ccgctgcgca aaaccaaggg atccgctgca
      601 tcatcgacag ttctggcgaa gcgttaagtg cagcactggc gattggtaac atcgagttgg
      661 ttaagcctaa ccaaaaagaa ctcagtgcgc tggtgaatcg cgaactcacc cagccagacg
      721 atgtccgcaa agccgcgcag gaaatcgtta atagcggcaa ggccaaacgg gttgtcgttt
      781 cccttggtcc acaaggagcg ctgggtgttg atagtgaaaa ctgcattcag gtggtgccac
      841 caccggtgaa aagccagagt accgttggcg ctggtgacag catggtcggc gcgatgacac
      901 tgaaactggc agaaaatgcc tctcttgaag agatggtccg ttttggcgta gctgcgggga
      961 gtgcagccac actcaatcag ggaacacgtc tgtgctccca tgacgatacg caaaaaattt
     1021 acgcttacct ttcccgctaa caaaaacatt cccccagcat tgggggaatc accaccaacc
     1081 tgtcggcaac gcgtttctcc gactatgctc aaaagtcatg tgataacaaa ggggtgaact
     1141 atggccagtg gcgatcttgt ccgttatgtc ataaccgtca tgttgcatga ggatacattg
     1201 actgaaatta acgagttgaa taattacctg actcgcgacg gttttttgct caccatgacg
     1261 gatgatgagg gaaatatcca tgagctgggg actaacactt ttggacttat cagtacccaa
     1321 agtgaagaag aaattagaga actggtttcg gggcttaccc aaagtgcaac cggcaaagat
     1381 cctgaaatca ccatcacgac ctgggaggaa tggaatagca acagaaaata aatggttttt
     1441 gggcaataat cagtctgtgg tgtgcgttag ctcgtgtttt tacaccgcat tcttgcgcta
     1501 accttatgat ctggcagaca acatgggaga gacatcatgt ggcaggcaat cagtcgtctt
     1561 ttgagcgagc agttaggtga aggcgaaatc gaactgcgta atgaactgcc tggcggagaa
     1621 gtccatgccg catggcattt gcgctatgca ggacatgact ttttcgtcaa atgtgatgaa
     1681 agggaactgc ttcccggttt taccgccgaa gccgaccaac tggagttact gtcgcgtagt
     1741 aaaaccgtca ccgtgcctaa ggtttgggca gtaggcgctg accgtgacta cagttttctg
     1801 gtgatggatt atctcccacc tcgtccgctg gatgcgcata gcgcatttat tcttggtcag
     1861 caaattgcgc gtttacatca atggagtgac caaccacaat ttggcctcga ttttgataac
     1921 tcgctctcca caactccaca gcccaacacc tagcaacgtc gctggtcaac gttttttgct
     1981 gaacaacgga ttggctggca gttggaactg gcagcagaga aagggatcgc tttcggcaat
     2041 atcgacgcca tcgtcgagca tattcagcag cgtctggcct cgcatcaacc gcagccttct
     2101 ctgttgcacg gcgatttatg gtccggcaac tgtgcgctgg gtccggatgg cccgtacatt
     2161 ttcgaccctg cctgctactg gggtgaccga gagtgcgacc tggcgatgtt accgctgcat
     2221 actgaacagc cgccacaaat ctatgacggc tatcagtcag tatccccgct acctgccgat
     2281 ttccttgaac gtcaaccggt ttaccaactc tacacgcttt taaatcgtgc aagattattt
     2341 ggcggtcagc atttggttat tgctcagcag tcattggata gattattagc agcatgatat
     2401 gggttgagga taatggccgc tccgtgcggc cttttgatta aataaaccca agcagagaaa
     2461 agaacacata acctgcagca atacaaataa ccggcaggat atatagtgag aaaaactgca
     2521 ggaaaatagt atggtgcgga acaacaatac gggactcaat ttgttcacgc gttagcccct
     2581 cttccccttt ggctttttcc agaatgagtt gatcttcaac accttcccgc aagaagcgcg
     2641 cctggcgact catccgggca ccggaatcct gcaacgccag tccgacaaaa atcaggatga
     2701 aaatcaccca gaacataacg ttcacgccac cattaaaatt gggcgtcggg gagttatacc
     2761 agaacagatt caaaaaaggc gtattcgcct gcatcatatc gatcatgaca tgagtaaagt
     2821 cgagcatgac cgcattaatg ccttcctggt tttcctgccg ggtattcata aatttcagca
     2881 aagaaatcaa tgtagagatc aatgcaggta taaaaatcac ccaacccaaa atccttttca
     2941 aaacagcaat gcgtccagct tgttgatacg tcatgagttc tccttgatta agacgcgtcg
     3001 tttcacttag tttacctgta gatatctgtt ttcgcccatt ctttaaaggc gatatgatag
     3061 gcgcttaatc ataagcacgg cttaatacct tacacataat gctctaaagg agaggttgtt
     3121 atgtcaaccc cgcgtcagat tcttgctgca atttttgata tggatggatt acttatcgac
     3181 tcagaacctt tatgggatcg agccgaactg gatgtgatgg caagcctggg ggtggatatc
     3241 tcccgtcgta acgagctgcc ggacacctta ggtttacgca tcgatatggt ggtcgatctt
     3301 tggtacgccc ggcaaccgtg gaatgggcca agccgtcagg aagtagtaga acgggttatt
     3361 gcccgtgcca tttcactggt tgaagagaca cgtccattat taccaggcgt gcgcgaagcc
     3421 gttgcgttat gcaaagaaca aggtttattg gtgggactgg cctccgcgtc accactacat
     3481 atgctggaaa aagtgttgac catgtttgac ttacgcgaca gtttcgatgc cctcgcctcg
     3541 gctgaaaaac tgccttacag caagccgcat ccgcaagtat atctcgactg cgcagcaaaa
     3601 ctgggcgttg acccactgac ctgcgtagcg ctggaagatt cagtaaatgg catgatcgcc
     3661 tctaaagcag cccgcatgcg ttccattgtc gttcctgcgc cagaagcgca aaatgatcca
     3721 cgttttgtat tagcaaacgt caaactctca tcgctgacag aactcaccgc aaaagacctt
     3781 ctcggttaat gaccaggggc agtgatctcg ctgcccctgg ttctttatct gaatttgcat
     3841 tcaactgacg gattaatcgt caatttaaga gaaagagtta caccgtcacc acttccgtgc
     3901 actgtataaa aatcctatac tgtacgtatc gacagtttag tgagttttat catgacggcg
     3961 gaaggtcacc ttctcttttc tattgcttgt gcggtatttg ccaaaaatgc cgagctgacg
     4021 cccgtgctgg cacagggtga ctggtggcat attgtccctt ccgcaatcct gacgtgtttg
     4081 ttaccggaca tcgatcaccc aaagtcgttt cttgggcagc gattaaagtg gatatcaaaa
     4141 ccgatcgccc gcgcttttgg gcatcgtggt tttacccaca gtctgctggc ggtatttgcg
     4201 ctgctggcaa ccttttacct taaggttcct gaaagctggt tcattccggc tgatgcgcta
     4261 caaggaatgg tgctgggtta tttaagccac atacttgccg atatgctgac acccgccggt
     4321 gttcccctgc tctggccatg tcgctggcgt ttccgcttgc ctatcctggt tccccaaaag
     4381 ggcaaccaac tggaacgttt tatctgcatg gcattatttg tctggtcggt atggatgccc
     4441 cattcattac ccgaaaacag cgcggttcgt tggtcatcgc aaatgatcaa taccttgcag
     4501 atccagtttc atcggcttat taagcatcag gttgaatact aaaaaggcaa aaatcacctt
     4561 tctggaataa gcaattccat ttgaatataa gagccagctc acagttctgt taatcttgcg
     4621 ccaacactat gactgctacg cagtgataga aataataaga tcaggagaac ggggatgaac
     4681 tttccattaa ttgcgaacat cgtggtgttc gttgtactgc tgtttgcgct ggctcagacc
     4741 cgccacaaac agtggagtct ggcgaaaaaa gtgctggtgg gtctggtgat gggtgtggtt
     4801 tttggccttg ccctgcatac catttatggt tctgacagcc aggtacttaa agattctgta
     4861 cagtggttta acatcgttgg taacggctat gttcaactgc tgcaaatgat cgttatgccg
     4921 ttagtcttcg cctctattct gagcgcggtt gcccgtctgc ataacgcatc tcagttaggc
     4981 aaaatcagtt ttctgaccat cggtacgctt ttgtttacca cactgattgc ggcgctggtc
     5041 ggtgtgctgg tcaccaacct gtttggtttg acggctgaag gtctggttca gggtggtgca
     5101 gaaactgcac gtctgaacgc catcgaaagt aactatgttg gtaaagtctc tgacctgagc
     5161 gttccgcagc tggtcttgtc ctttatcccg aaaaacccgt ttgccgatct gaccggagcc
     5221 aatccgacgt caattatcag cgtggtaatt tttgccgcat tcctcggtgt agctgcgctg
     5281 aaactgctga aggatgatgc gccgaaaggt gaacgcgtct taaccgctat cgataccctg
     5341 caaagctggg tgatgaaact ggttcgcctg gtcatgcagt tgacccctta cggcgttctg
     5401 gcactaatga ccaaagtggt tgcaggttct aacctgcaag acatcatcaa actgggaagt
     5461 ttcgttgtcg cgtcctacct cggtctgctg attatgtttg cagtgcatgg cattctgctg
     5521 ggcattaatg gcgtgagtcc gctgaagtac ttccgtaagg tatggcctgt gctgacgttt
     5581 gcctttacca gccgttccag tgctgcgtct atcccactga atgtggaagc acaaacgcgt
     5641 cgtctgggcg ttcctgaatc catcgccagt ttcgccgcct ctttcggtgc gaccattggt
     5701 cagaacggct gcgccggttt gtatccggca atgctggcgg tgatggttgc gcctacggtt
     5761 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     5821 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     5881 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     5941 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     6001 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     6061 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     6121 atgttccatc gcggcgatgc atactgactc atatttaaac agatcgtcag cgaaaaaaat
     6181 gtccaactta tcaatacatt cctggctcaa tgaatgtgcc tgtagtgaga agcttaaaac
     6241 ctcatacccg agacgttttc taagtgaact atctggaata ctcataatat cttcaagcac
     6301 accacttccc attcccggaa gatttagtgg a
//
LOCUS       DAZFFI010000192         6323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFFI010000192 DAZFFI010000000
VERSION     DAZFFI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.194"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2235
                     /locus_tag="ACHJ12_004761"
     CDS             <1..2235
                     /locus_tag="ACHJ12_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4992428.1"
                     /translation="SGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKTTSEQE
                     ALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGRVNTMV
                     DGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINALAGSVN
                     MRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVMAAVSG
                     SSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYENKFTR
                     RDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRSDALDI
                     NNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSGQQDIS
                     ALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDDKVGGFNPSV
                     QLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAGFNIDT
                     RDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDWEGISDEYSD
                     NMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGAGDMTEL
                     PRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQIPTII
                     DLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRTWIFGG
                     DIRF"
     gene            2304..3479
                     /gene="senB"
                     /locus_tag="ACHJ12_004762"
     CDS             2304..3479
                     /gene="senB"
                     /locus_tag="ACHJ12_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO4992429.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4109..4567)
                     /locus_tag="ACHJ12_004763"
                     /pseudo
     CDS             complement(4109..4567)
                     /locus_tag="ACHJ12_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(5007..5393)
                     /locus_tag="ACHJ12_004764"
     CDS             complement(5007..5393)
                     /locus_tag="ACHJ12_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO4992430.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(5390..5614)
                     /locus_tag="ACHJ12_004765"
     CDS             complement(5390..5614)
                     /locus_tag="ACHJ12_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO4992431.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            5680..6035
                     /locus_tag="ACHJ12_004766"
                     /pseudo
     CDS             5680..6035
                     /locus_tag="ACHJ12_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6268..>6323)
                     /locus_tag="ACHJ12_004767"
     CDS             complement(6268..>6323)
                     /locus_tag="ACHJ12_004767"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO4992432.1"
                     /translation="GTVASSSSSELTGIAVQ"
BASE COUNT         1780 a         1492 c         1466 g         1585 t
ORIGIN      
        1 tcaggcatat tattgctcag ctgcggtgct tactcacaat ccatcagtga aaaaactaat
       61 tccgacaaaa aaggagcggc agaattcagt ccgctcagcg tttctgtcgg gaagacgacc
      121 agtgagcagg aagctctcga gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac
      181 ctgcaatcac ttgacgcaac agtgcgtagt atgcccggta cttatactca aatagatcct
      241 ggtcagggag caatcagtgt gaatattcga ggcatgagcg gatttggtcg tgtaaacact
      301 atggtcgatg gtattaccca gagtttttac ggaacctcta cctccggaac aacgacgcat
      361 ggttcaacta acaatatggc tggcgtactt atagatccta acttactggt agcagttgat
      421 gttacacgcg gtgacagcag tggctctgaa gggatcaacg cccttgccgg tagtgtaaat
      481 atgcgtacta ttggcgttga cgatgtaata tttaacggta atacatatgg ccttcgttca
      541 cgtttctctg tcggtagtaa tgggctggga cgcagcggaa tgatcgccct tggtggaaaa
      601 agcgacgctt ttacggatac gggaagcatt ggcgttatgg ctgctgtgag cggcagttct
      661 gtgtactcta atttctcaaa tggttctgga attaacagca aagagtttgg ttatgataaa
      721 tatatgaagc agaaccccaa atcccaactg tataaaatgg atatcagacc agacgaattt
      781 aacagcttcg aactttccgc tcgaacctat gaaaataaat ttacacgtcg tgatataacc
      841 agtgacgact attacattaa atatcattac accccttttt ctgaattaat tgactttaac
      901 gtaacggcca gtaccagtcg cggtaatcaa aagtatcgtg atggctcgct gtatactttc
      961 tacaaaacct cagcgcaaaa tcgttctgac gcgctggata tcaacaatac cagccggttc
     1021 actgtcgcgg acaatgacct ggagtttatg ctgggcagca aactgatgcg tacccgctat
     1081 gaccggacca ttcactcagc ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac
     1141 aatccgttcg caccctccgg ccagcaggat atttcagcgc tgtataccgg gctgaaggtt
     1201 acgcgcggca tctgggaggc agatttcaat ctcaactaca cacgtaacag gatcacaggg
     1261 cacaagcccg cctgcgattc acgcgttatc tgcgtgccac agggtagcta cgatattgac
     1321 gataaagtgg gtggcttcaa cccttcagtt cagctttctg ctcaggtaac accatggctt
     1381 cagccgttca ttggctacag caaatccatg cgcgccccga acatccagga gatgttcttc
     1441 tctaattcag gaggcgcatc catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg
     1501 caggcgggtt ttaacattga taccagagat ttagtggtcg aacaggatgc cctgcgcttt
     1561 aaggctctgg cgtaccgcag caggatccag aactacatct acagcgagtc ttatctggtt
     1621 tgttctggag gtcgtaaatg cagtatggct gaggtgattg gcaatgactg ggagggcatt
     1681 agcgatgaat acagcgacaa tatgtacatc tacgttaact cggcaagcga cgttatcgca
     1741 aagggcttcg aactggagat ggattatgat gcaggttttg cttttggccg actctctttc
     1801 agccagcagc aaacagacca gccaacctcc atcgccagca cctactttgg cgcaggggat
     1861 atgaccgaac tgcccagaaa atacatgacg ctggatactg gtgttcgctt cttcgataac
     1921 gcgttgaccc tgggcactat cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat
     1981 tttgagcagg acgaacatac cggcgcaata atcaaacagg atttgccgca gatcccaacg
     2041 attatcgatc tctatggtac ttacgagtac aaccgcaacc tgacactgaa actttcggta
     2101 caaaacctga tgaacagaga ttattcggag gcgctgaata agctcaacat gatgccaggt
     2161 cttggtgacg agacccaccc agccaattcc gcgcgtggca gaacatggat atttggcggg
     2221 gacattcgtt tctgatgaca atttccgcta ggttcagaca atacgttttc tctcttatgt
     2281 caattttatt gcaggaacga aaaatgaata ttttcacttt atccaaaaca ccgctatacc
     2341 tgttaatttc actattttta cccacgatgg ccatggctat cgatccacct gaacgcgaac
     2401 tttcgcgatt tgccctgaaa acgaattacc ttcagtcccc tgatgaaggc gtctatgaac
     2461 tggcgtttga taatgccagt aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg
     2521 aagccaataa aggctatctg tattcgttta attcagattc gctgaaagtc gaaaataaat
     2581 acacgatgcc ataccgggca ttttcgctgg cgataaatca ggataaacat cagctctata
     2641 tcggacacac ccagtcagcg tccctgcgta tcagtatgtt tgacacccca accggcaaac
     2701 tggtaagaac cagcgacagg ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg
     2761 agcatttgcg ccatatggtt tacagccagg attccgatac cctgtttgtg agttatagca
     2821 atatgctgaa aacggccgag ggcatgaagc ctctgcataa gctgttaatg ctcgacggga
     2881 cgacgcttgc cttaaaaggc gaggttaagg atgcttacaa aggtacagcg tatggtctga
     2941 cgatggatga aaaaacacag aaaatctacg ttggcggaag agattacatc aacgaaattg
     3001 atgcgaaaaa tcagacgctg ctgcgtacca tcccgttgaa agatccgaga ccacaaatca
     3061 caagtgtgca gaatctggcg gtggactccg cttctgaccg tgcctttgtg gtggtattcg
     3121 accatgacga tcgttccggt acaaaagatg gactctatat ttttgactta cgcgacggta
     3181 aacagcttgg ctatgtgcac acaggagccg gagctaacgc ggtgaaatac aatccgaaat
     3241 ataacgaact gtatgtcacc aacttcacta gcggcaccat cagcgtagtg gatgccacca
     3301 aatacagcat cacccgtgaa tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg
     3361 gtgatatgga taccctttac attggcatca aagaaggctt taaccgcgat tgggatcctg
     3421 atgtgtttgt ggaaggagct aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat
     3481 cgggggaaaa catgggataa aaactccgtt attccactaa actggccgga ctaggaaaag
     3541 caaacatctc tgtcctgccg gtacgcctgt tacgccgctc catgaggctg aggaatctcc
     3601 agaaaacgag acaggcacag cattgtacga tcaactgatt tgtgccaata attcaatgaa
     3661 atcacagcta tgcctgttca tggggttcta ggccggaacc ccagattttt ccgattgttt
     3721 gttttctaac tgattattca gtagttttag agggctgacg ctcagtggaa cgaaaactca
     3781 cgttaagagg ttttgactat gataaaagtc aaagtagggg tgagtttgga tatggaaatt
     3841 attgtactat aagggcttcc ccgaacagtc aacgtcggta tttatggttt gtctgcacta
     3901 cagttaaaaa taaaggggag agactgcggt actataaacg gtcataataa atcgttatcg
     3961 attcggaccc tgatgaaaga cgcgcgtgag gctcttgttc gttaagcgga tacgtatatc
     4021 ctgtacaata atcagatata cctcacgggg tctaaccctt tatcatcaac gagttgcagg
     4081 tcagatagtc atacggttgc tgcgtgcttt agctggctga atacagtctg aaatcatcac
     4141 cgtaatgcaa tgatgcccag cacagccttg catttttggc ggcgatggcc acaacagcgc
     4201 gccagtatcc tctgcgttcg accagtgaac agacccaacg actgaatgag tcagtccttt
     4261 tctcagcgcc aatcagtact gaacgagcac cctgaaccag aagcgttcgc agatacgaat
     4321 caccggcttt tgttatcctg cctagcttcg actttccgcc gctgctgtat tgtgatggag
     4381 tcagtcccag ccaggccgcc agttgccgtc catttttaaa atcatgtgca ttaccgatac
     4441 aggcaaccag cgcacaggca gttgtgggac caattccttt tagctccatc agccgctgac
     4501 tgcggtgatc tgttcttgct atacgggaca aaattttgtc atattcagtg atgttagctt
     4561 caatgcgggg tcagtttgga gatcgtaaaa ttttgttcgt taagcacatt atttaaccaa
     4621 tagatatgcg taacggattg attttgcgtc aacggctatc gcttttaagt tatcgccatt
     4681 gaggtattgc atttaggtta tccgatagag gtcaaatcct ctgaaaacgc cgtatagcac
     4741 gggagtacac cctaccgcaa tagcgcaacc gtacaatatt gtacgtttcc caaacgcagc
     4801 ctaggaagca cgtcgtcagt attggagaaa acttacaaac gctcgccgca gccccagcga
     4861 ccgaacgtta gtgagcgagt gggcgaggaa gcggaaaagt gtcaggtctg aattgagcac
     4921 atacgccaga aattatatag caaaaagata tatgattttt acattgctca gtagattgta
     4981 aaatgctgct gattggatat ctatatctaa tttaaaataa tggctttaag atggtcagaa
     5041 accatcttcc agttatattc tcctgttgca gcattcaaag cgataatttg catttcatca
     5101 aacgaaatac gcgaactaat aatatgtata ccattccgtt ttaaaaaaac ctgtgcaaca
     5161 aacaaggctg ttcgcttatt tgcatcgttg aaaatgtgac cacgagcaat agcaactaaa
     5221 tacacggcag ccagatccat aatatctgtc acaccttcat aatgatacat gttcaaaaca
     5281 cgatgaatga tcgcgtcagc gcgaccgggt tctggcatac ccgccacgcc accatcacgc
     5341 gaaatcaagc ggtcatgaaa ctcaatcatt tcctgtgaac taacgaattt catcgcttag
     5401 aaagctccgt ataggccgtc ccatgacttt gcatgatctc agcaaactca gcatcaagag
     5461 ccgctttacg gtatcgttca aaagcgtctt tatcaaccag tacagccgcg ccacctttac
     5521 ggcgctgaat ctcaacaggc tgattttcat caaccgccag ctcgagcaac gaggccaggt
     5581 tatcgcgagc atggctatag ttacatgtaa acatagtgct tcctctttga gttgtacaat
     5641 taaattgtac aacagttatc ttcaatatga aatcaccgtt accgctggcg catcctgacc
     5701 caacttaacc ggggagaaag tcgccatgcc gttgccagag ctatctgtca cggtcagaaa
     5761 ggtgagataa gaaagcgata taccgatggt caagaagatc aactgggcgc actggggctg
     5821 gtgactaacg ccgtcgtatt atggaacact atttatatgc aggctgccct ggaccatttc
     5881 agggcacagg gggaaatcgt gaatgatgaa gatatcgcac gcctctcacc actttgccac
     5941 ggacacatca atatgctcgg ccatattcct tcacgctggc agaactggtg actaaagggc
     6001 aaaaagaggc atcggaggaa gaaaacattg cttaacgtga gttttcgttc cactgagcgc
     6061 cagcccccta attctctata tccaaactga ccccaatagg tgacgggtta tgtgacgaaa
     6121 tctgatgtag aaatcgttgt ttcagtgaca gtcactcaat cggtcattta tgtgacaccc
     6181 cacgccgtta ctggtcgcgg aaaaatccag cggaaaaatc caggtttccg gcttgggacc
     6241 ccatctcaag agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagaaga
     6301 cgaagatgac gcaacggttc ctc
//
LOCUS       DAZFFI010000193         6277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFFI010000193 DAZFFI010000000
VERSION     DAZFFI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.195"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            159..374
                     /locus_tag="ACHJ12_004768"
     CDS             159..374
                     /locus_tag="ACHJ12_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992433.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            786..977
                     /locus_tag="ACHJ12_004769"
     CDS             786..977
                     /locus_tag="ACHJ12_004769"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992434.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1119..2138
                     /locus_tag="ACHJ12_004770"
     CDS             1119..2138
                     /locus_tag="ACHJ12_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO4992435.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2859..3074
                     /locus_tag="ACHJ12_004771"
                     /pseudo
     CDS             2859..3074
                     /locus_tag="ACHJ12_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3249..5219
                     /locus_tag="ACHJ12_004772"
     CDS             3249..5219
                     /locus_tag="ACHJ12_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFO4992436.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5238..6029
                     /locus_tag="ACHJ12_004773"
     CDS             5238..6029
                     /locus_tag="ACHJ12_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFO4992437.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1884 a         1392 c         1508 g         1493 t
ORIGIN      
        1 ttcatataat tctttaaaca gcgaacttat agccaggatt caatagatat agtaacagga
       61 acatcagtaa gcgatgagat ggtcttcccc ctccttcacg acaaccttta attacgtcat
      121 gatggataaa ctttaaccac aaaagtaaga gggataccat gtctcaacag aaaatcaccg
      181 gcatcgatct ggctaaaacg aacttttatc ttttcagtat caatgctcat ggcaaacccg
      241 caggaaggat caaactttct cgcaatcaac tgcttaactg gctggtgcag cagcctaaaa
      301 tgactgttgc gatggaggct cgcgggcatc ccatcactgg gccagggaaa tcaggaaact
      361 cgaccatgat gtaatatttc ttccggcgca acatgttaaa gcttatcagc gacgccagaa
      421 aaatgattat gacgacgctc aggcaatagc tgaggcctgc caacatggaa ctatacgccc
      481 cgtaccaata aaaactctgg agtaacagga cgtccagact tttttgaaaa tgcgccggtt
      541 ggtttcgatg gaaaggacac aactgataaa tcacgttcgt ggacttcttg ctgaatatgg
      601 cattgtgttc agtaaaggcg caacagaatt acgccaaaaa ttaccggcat tacttgagga
      661 tgcggaaaac gaactcactg ataccatgag acgttattgc atcggcagta tatacggttg
      721 attacactag ataatgaact ggagtggtat gattctgaac tgaaaaatat gtccgccaag
      781 atcctatgtg caaacgcttg ttagccattc ctagattcag ccctctggtt agtcaggcaa
      841 taaaaagctg gatgggggat ggaaaacagt ttagacgagg aagagatgca tctgcgactc
      901 tgggactggt tccccgacag ttcagcacag gtgggaagca ggttcttctg ggtataacaa
      961 aatttggaga aatgtaatga acacatccca ccccattaag tcaggtgctc tgtgatggat
     1021 gaagggaaat actggaaggt gtggagcctc gcagatttcc ggcatcatag tgcccaatac
     1081 aggatgcata aagctccctc agactggaga ctccacaaat gaaatataca ccggttggcg
     1141 ttgatatcgc aaaacatctg attcaggttc atttcatcga cgagcacaca ggtgaagtga
     1201 ttgataagca actacgtcgt caggattttc tgatgttctt ctgtaatcgg gaaccctgcc
     1261 tgattggtat ggaggcctgt agaggctctc agcactgggg gcgtgaactg acaaagctgg
     1321 ggcataaagt ccggttgtta caggcccact ttgttaaggc attcgtcatg ggaaataaga
     1381 atgatgtgat ggatgcccgg gctatctgga tggctgttca gcagccaggt aaggacatcg
     1441 cggttaaaac agaagaacaa cagtcagtgc tggttttgca ccgttcccgc agacaattgg
     1501 ttaagttccg caccatgcag attaattctc tgcacggcac gctcctagaa tttggtaaaa
     1561 ccattcacaa aggtcgggca gcgatggagc gagagctccc tgaagcactg gagcggatga
     1621 aagagagact gccgccgtat ctcattactg tgctggaaaa ccagtacaac cggctgaatg
     1681 agctggactc gctgatagag gatattgaaa aaaagcttac cagcgtggcg agacagaatg
     1741 aaacctgtaa gcggttgctg gatattcccg gcgttgggcc acttattgca acggcagcgg
     1801 tggccaccat gagggaagca tcagcgttta aatcagggcg agagttcgcg gcttatgttg
     1861 gtctggtgcc caaacagact ggctcaggag ggaaaatccg cctgctggag ataagcaaac
     1921 gtggtaatac atatctgcga gccttgttta ttcatagcgc aagggcggca gcattactgc
     1981 ccaaagaacc tggtccgtgg ataaccgaac tgaaaaaacg tcgtccaacc agcgtggcaa
     2041 tcgtcgccat ggcaaacaaa ctagcacgaa cagtatgggc gatagcagct catggaagta
     2101 aatacgacaa gaatcatgtc agtatcagat catattaatc gctgataccg ataaatatta
     2161 acttttaatg aaaggatgaa tactgaaagg taactctggc ggccatagtg atgacaacga
     2221 caggtaagac cgggactcac taaacctgaa tattgtgttg agctaaaagc tcgctggaga
     2281 aatgaggtgt gagtcagcga attacatagg ggctcgcagc ttcatggctg caaaaaagcc
     2341 ggatataaag ctgcaaccta cccgtcactg tcaaggcaat ggatgccttg caacgggatg
     2401 tgttcatata atcaatatgt cagatctatg ctgatacagg gtgccagagc ggtattgttc
     2461 agagcctcag agaaaactga ttcacttcgt gtgtgggtta attgtattcg ggaaaagcgt
     2521 ggcttcaacc aggcagttgt tgctcttgca aataaactcc ttcggatcgc ctgggcaatt
     2581 atcgcccgca atgagatgta tttgcccgca gcaaaacaaa atcaataatc tacgtaaaat
     2641 cagtaggagt aaagcttacc ctgaaattgc aaagaatgca gaatgatgaa aaacaggtca
     2701 gaccgacgtt acgaaaacct gcaagatgag ccaggccagt aaggccgagt aagcgataag
     2761 gtcggagcgc gcgaatgtct catcaaggcc agaagcaata acttcatgaa caaaccggat
     2821 atacgaaagt aaacctgtcc ctatcatagt tgctgtactc ttgcaatacg ggggaagacc
     2881 atctcatcgc ttacgaacaa ttcgtgaaaa cgatgaagaa agactacatc gcgtgcatgc
     2941 cgaaatctaa tgtaagaacg gcattgcata atcttgcagt ggagatcggg tattacaatg
     3001 aaaaccatcc gcatagtgcg ttaggttatc tcccaccaag ggaatatcaa cgtcagcgaa
     3061 taacgtcacc ttagatgcag tagttgtcag gaaatagtga ctcaagggca ggcttaccat
     3121 atacactctt agtggttcga ctgggtatta gaatagctat taacagatta gcactgaaca
     3181 ctgcgtaata gtggttgcac aaacaaaatg ttatgttata acattgctgt attgacatat
     3241 attttcgaat gtatatgaac gtaatcagaa ctgtcatttg tacattaatc atacttccgg
     3301 tggggttaca ggcagcgaca agtcattctt ctatggctaa agatacaatc accgttgtcg
     3361 cgacaggaaa tcagaacacg gtatttgaaa cgccgtcgat ggtcagtgtc atcacgaatg
     3421 acacgccatg gagtcagaat gcggtcacat cagccggtat gctgaaaggt gttgcgggtc
     3481 tcagccagac tggtgcagga cggaccaatg ggcagacctt taatctacgc ggctatgaca
     3541 aaagcggggt acttgttctt gttgacggcg tccgtcaact cagtgacatg gcaaaaagca
     3601 gtggcactta tctggatccg gcactcatca aacgcatcga agttgtccgc ggaccaaact
     3661 ccagtctgta cggcagtggc gggctggggg gggtggtgga cttcagaact gccgatgctg
     3721 cagattttct tccccccgga gagacaaatg gtgtaagtct gtggggaaat atcgccagtg
     3781 gtgaccacag cacaggctcg gggctcacct ggttcggtaa aaccggaaaa acagatgcgc
     3841 ttctttctgt cattatgcgt aaaagaggaa atatctatca gagtgatggt gagcgtgcac
     3901 ctaacaagga aaaacctgca gccctgtttg cgaaaggctc tgtcagtata acagacagta
     3961 acaaagcagg tgccagcttg cgtctctacc agaataacac cactgaaccg ggcaatccca
     4021 ctctgacaca tggtgacaac ggcctgcgtg acagaaaaac agcacaaaat gacgtgcagt
     4081 tctggtacca gtactcccct gcggataaca gcctcatcaa tgtaaagtca acgttatatc
     4141 tcagtgatat cactatcaag acaaacggtc acaacaaaac agcagaatgg cgaaacaaca
     4201 gaacctccgg tgttaatgtt gtcaacagga gccatactct gatttttccg ggagcccatc
     4261 agttaagtta tggcgccgaa tattaccgtc agcagcagaa gccggaaggc tctgccacac
     4321 tatatccgga aggaaacatt gactttacat cgttgtattt ccaggatgaa atgacaatga
     4381 aaagcaaccc ggttaacatt atcgtcggtt cccgttatga ccggtacaag agcttcaatc
     4441 cccgagccgg aaaactgaaa gccgaacgcc tgtcccccag ggcggcgatt tcagtctcac
     4501 caacagactg gctgatgatg tacggctcca tatcctctgc attccgtgcc cccacaatgg
     4561 cagaaatgta cagggatgat gtacattttt accgcaaggg taaacccaat tactgggtac
     4621 ctaaccttaa tctgaaacca gaaaataaca ttacccgtga gattggcgca ggtattcaac
     4681 tggacggcct gcttacagac aatgaccggc tgcagttgaa aggcggatat ttcggaacgg
     4741 atgccagaaa ctatattgcc acgcgcgtgg atatgaaacg gatgcgttct tattcttata
     4801 atgtatcccg ggcccgtatc tggggatggg atatgcaggg taattaccag tctgattatt
     4861 ttgactggat gctttcttat aaccgcacgg aaagtatgga tgccagtagc agggaatggc
     4921 tgggctccgg caatcctgac acactcatca gtgacatcag tatacctgtt ggtcatggag
     4981 gagtttctgc cggatggcgt gctgaacttt cggcagcagc cacgcatgtg aaaaaaggcg
     5041 atccccatca ggctggctat gccgtacatt ccttttcact gtcttataag cctgtaagtg
     5101 ttaaaagctt tgaggcatca gtgactctgg ataatgcctt caacaagctt gccatgaatg
     5161 gcaaaggtgt gccgctttca ggcagaactg tcagtcttta tacccgttat cagtggtaac
     5221 ctcgcaggaa ataaatcatg atgaaaaata caggctatat cttagctctt tgtctgacag
     5281 catcggagca tgccctggcc cacgatgtct ggattaccgg taaacaggca gagaacaacg
     5341 ttaccgcaga gattggttat ggtcataatt tcccctcaaa gggcacaatt cctgacagaa
     5401 ggaatttctt tgaaaatccc cggctttata acgggaaaga gataataacg ctgaagccaa
     5461 cgtccacgga ttatgtctat aaaactgagt ctgcagccaa cggtaatagc tacgttctgt
     5521 caacgaaaat gaaaccggga tactggtcga gaacctcgtc aggatggaag cctgtcagtc
     5581 gggagggcag aaatgatgtg gcatactgtg agtttgtgac taaatatgca aaatctttta
     5641 ttcctggtga acagcagatg ccagcacaac tctatcagtc tccaacaggg gatgagcttg
     5701 aaatcattcc attatccgat atcagtcgtt tcggtgaaga tgtgaagctg aaagttctgt
     5761 ataaaacatc cccgcttgcc ggagctactc tggagcttga ctctgtcagt tatctgaaat
     5821 catcccgtca tacccatgca gctgagcata aacattcggc tcataaagca gaacttacct
     5881 ttgtaagtaa tgaggacggt atcatcactg taccttccct tcatgtcggg caatggctgg
     5941 ctaaagtcaa aaataagaaa gtatttccgg acaagagtct gtgtgatgaa actgtcgatg
     6001 tggcaacctt aagcttctcc cgaaattaat aatactattc ctgccctgat ttatgggcag
     6061 ggtattatca atacgagaat agcctaccga catccgtgta aaaaaccaca taatgatttt
     6121 tcatatccat ttccagatat acttatgtaa agatcacctc caaaaaagcg taatagtaat
     6181 aaaaatccct gaagcaccct gttcaaacca gaaggaatac ataacaagac aataaaatgt
     6241 gaaatccatc acagcaaaga actctttgtt tataata
//
LOCUS       DAZFFI010000194         6061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFFI010000194 DAZFFI010000000
VERSION     DAZFFI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.196"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            202..1329
                     /gene="ompC"
                     /locus_tag="ACHJ12_004774"
     CDS             202..1329
                     /gene="ompC"
                     /locus_tag="ACHJ12_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4992438.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNGSYISNGVARNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1441..2496
                     /gene="apbE"
                     /locus_tag="ACHJ12_004775"
     CDS             1441..2496
                     /gene="apbE"
                     /locus_tag="ACHJ12_004775"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4992439.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2570..3634
                     /gene="ada"
                     /locus_tag="ACHJ12_004776"
     CDS             2570..3634
                     /gene="ada"
                     /locus_tag="ACHJ12_004776"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4992440.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3634..4284
                     /gene="alkB"
                     /locus_tag="ACHJ12_004777"
     CDS             3634..4284
                     /gene="alkB"
                     /locus_tag="ACHJ12_004777"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4992441.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAAGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4360..6003
                     /gene="yojI"
                     /locus_tag="ACHJ12_004778"
     CDS             4360..6003
                     /gene="yojI"
                     /locus_tag="ACHJ12_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4992442.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLIQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1447 a         1528 c         1639 g         1447 t
ORIGIN      
        1 aatcttaaaa agttcccttg aatttacatt ttgaaacatc tatagcgata aatgaaacat
       61 cttaaaagtt ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac
      121 ttgccgactg attaatgagg gttaatcagt atgcagtggc ataaaaaagc aaataaaggc
      181 atataacaga gggttaataa catgaaagtt aaagtactgt ccctcctggt cccagctctg
      241 ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa caaattagat
      301 ctgtacggta aagtagacgg cctgcactat ttctctgaca acaaagatgt agatggcgac
      361 cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca gctgaccggt
      421 tacggccagt gggaatatca gatccagggc aacagcgctg aaaacgaaaa caactcctgg
      481 acccgtgtgg cattcgcagg tctgaaattc caggatgtgg gttctttcga ctacggtcgt
      541 aactacggcg ttgtttacga cgtaacttcc tggaccgacg tactgccaga attcggtggc
      601 gacacctacg gttctgacaa cttcatgcag cagcgtggta acggctttgc gacctaccgt
      661 aacactgact tcttcggtct ggttgacggc ctgaactttg ctgttcagta ccagggcaaa
      721 aacggtagcg taagcggcga aggcatgacc aacaatggtc gcgatgctct gcgtcagaac
      781 ggcgacggcg ttggcggttc tatcacttat gattacgaag gcttcggtat cggtgctgca
      841 gtttccagct ccaaacgtac tgatgatcaa aatggtagct acatcagcaa tggtgtagct
      901 cgtaactaca tcggtactgg cgaccgtgct gaaacctaca ctggtggtct gaaatacgac
      961 gctaacaaca tctacctggc tgctcagtac acccagacct acaacgcaac tcgcgtaggt
     1021 tccctgggtt gggcgaacaa agcacagaac ttcgaagctg ttgctcagta ccagttcgac
     1081 ttcggtctgc gtccgtctgt agcatacctg cagtctaaag gtaaaaacct gggtgtcatc
     1141 aatggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg cgcgacctac
     1201 tacttcaaca aaaacatgtc cacctatgtt gactacaaaa tcaacctgct ggacgacaac
     1261 cagttcactc gtgacgctgg catcaacact gataacatcg tagctctggg tctggtttac
     1321 cagttctaat ctcgattgat atcgaacaaa gggcctgcgg gccctttttt cattgttttc
     1381 agcgtacaaa ctcagttttt tggtgtactc ttgcgaccgt tcgcatgagg ataatcacgt
     1441 atggaaataa gctttacccg cgtggcactg ctggctgccg cgctcttctt tgttggttgc
     1501 gatcaaaaac cacaacccgc caaaacccac gctactgaag ttaccgttct tgaaggcaaa
     1561 accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg cagtgccgaa
     1621 cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc gacctataaa
     1681 aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg gccggtaagt
     1741 gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gcgcaaggac cgatggtgcg
     1801 atggatataa ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga acaacagccg
     1861 gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg cttacagcac
     1921 ctgacggtca tcaatcagtc gcatcagcaa tatctgcaaa aagacctgcc ggatttatat
     1981 gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg cttgatggag
     2041 caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag ccgtggtatg
     2101 aacggtgaag gccagccgtg gcgggtagcg attcaaaaac caaccgataa agaaaacgcg
     2161 gttcaggccg tggtggatat caacggtcat ggcattagca cctctggcag ctaccgtaac
     2221 tattacgaac tggacggcaa acgtctttct catgttatcg atccgcaaac cgggcgtcct
     2281 atcgaacaca atctggtatc cgtgacggtg attgccccga cggcgctgga agccgatgcc
     2341 tgggatactg gcttaatggt actcgggccg gagaaagcca aagaggttgt tcgccgggaa
     2401 gggctggcgg tctatatgat caccaaagaa ggtgatagct ttaaaacctg gatgtcgccg
     2461 cagtttaaaa gcttccttat cagcgaaaaa aattaaagcg caagattgtt ggtttttgcg
     2521 tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta tgaaaaaagc
     2581 cacatgctta actgacgatc aacgctggca atctgtctta gcccgcgacc cgaatgccga
     2641 cggcgaattc gttttcgccg tgcgtaccac aggcatcttt tgccgtccgt cttgccgcgc
     2701 cagacatgct ttgcgggaaa acgtctcctt ctacgcaaat gccagcgagg cactcgccgc
     2761 tggctttcgc ccctgcaaac gctgccaacc tgataaagcc aatgcccagc aacatcggtt
     2821 ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa cgttggaagc
     2881 cttagccgat caggtggcga tgagtccgtt ccatctgcat cggttgttta aagcgactac
     2941 cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc gcgaatcgct
     3001 ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg acagcagcag
     3061 ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc gtcacggtgg
     3121 cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgcg ctggggcgtt gcctggtggc
     3181 agaaagcgag cgggggattt gcgcgatatt actgggcgat gatgacgcga cactaatcag
     3241 cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga tgtttcagca
     3301 acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga cgttaccgct
     3361 ggacattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca ccataccttg
     3421 cggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga aagcggtacg
     3481 ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc ataataccct gtcatcgggt
     3541 ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta aagcgcaact
     3601 gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc cgatgctgaa
     3661 ccgtggcaag agccactggc ggctggagcg gtaattttgc accgttttgc ttttaacgct
     3721 gcggagcaac tgatccgcga tattaataac gttgccagcc agtcgccgtt tcgccagatg
     3781 gtcacccccg ggggatatac catgtcggtg gcgatgacca actgtgggca tctgggctgg
     3841 acgagccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa taaaccgtgg
     3901 cccgccatgc cacagagttt tcataattta tgtcaacgtg cggctacggc ggcgggctat
     3961 ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctgctggcgc gaaactgtcg
     4021 ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt ttctctgggc
     4081 ttacccgcaa tttttcaatt tggtggcctg aaacgtaatg atccgctcaa acgtttgttg
     4141 ttggaacatg gcgatgtggt ggtatggggc ggtgaatcgc ggctgtttta tcacggtatt
     4201 caaccgttga aagcggggtt tcatccactc accaccgact gccgctacaa cctgacattc
     4261 cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc gcgaagatgt
     4321 ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct tgtacttgtc
     4381 tggcggcagt atcgctggcc atttatcagt gtgatggcgc taagcctcgc cagtgcggca
     4441 ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc ggataccagt
     4501 ctgctggtgt tgccggagtt tcttggatta ttactgctgt tgatggcagt cactctcgga
     4561 tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg tagtgaattt
     4621 atcaagcgga ttctggatac ccacgtcgag cgcattgaac aactcggtag cgcctcgttg
     4681 ctggcgggat taaccagcga tgtgcgcaat atcaccattg cttttgtgcg tcttccggaa
     4741 ctggtgcagg ggatcattct cactatcggt tccgcggcgt atctgtggat gctgtcgggc
     4801 aaaatgttgc tggtaacggc tatctggatg gcgatcacca tctggggtgg ttttgtactg
     4861 gtggcgcggg tgtacaaaca tatggcgacc ctgcgtgaaa ccgaagacaa gctgtacacg
     4921 gattttcaaa ccgtactcga agggcgcaaa gagctgactc tgaaccggga acgcgccgag
     4981 tatgtgttta acaacctcta catccccgat gcgcaagagt atcgccatca tattattcgc
     5041 gcagacacct tccatcttag cgccgtgaac tggtcaaaca tcatgatgct gggcgctatc
     5101 ggcctggtgt tctggatggc gaacagtctc ggttgggctg ataccaacgt tgctgcgacc
     5161 tattcgttga cgctgttatt cctgcgtacg ccgctgcttt cggcggttgg cgcattgccg
     5221 acgctgctga cggcgcaggt ggcgtttaac aagctgaaca aattcgcgct cgcgcctttc
     5281 aaagctgagt ttccgcgccc gcaggctttt cccaactggc aaacgctgga gctgcgtaac
     5341 gtgacgttta gctatcagga taacgcgttt tccgttgggc cgattaacct caccattaaa
     5401 cgtggcgagc tgctgtttct gattggtggc aacggtagtg gaaaatcgac gctggcgatg
     5461 ttgctgacgg gcttgtacca gccacaaagc ggcgaaatct tgctggatgg caaacctgtc
     5521 agtggcgaac aaccggaaga ttatcgcaaa ctgttttcgg cagtgtttac cgatgtctgg
     5581 ctgtttgatc aactgctggg gccggagggt aaacccgcta acccgcaact ggttgagaag
     5641 tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg tattgttaac
     5701 ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct ggcagaagaa
     5761 cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt ccgtcgtgag
     5821 ttttatcagg tgttgctgcc gctgatacag gagatgggta aaactatttt cgctatcagt
     5881 catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa tgggcaactt
     5941 agcgagctga cgggcgaaga gcgcgatgcc gcttcgcgtg atgccgttgc ccggacggca
     6001 taatattacg acgctgacct ggcggactgc tgccgtcagg tcaatatgct aaaaatccct
     6061 t
//
LOCUS       DAZFFI010000195         5945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFFI010000195 DAZFFI010000000
VERSION     DAZFFI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.197"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..616
                     /locus_tag="ACHJ12_004779"
     CDS             170..616
                     /locus_tag="ACHJ12_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4992443.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            627..2078
                     /locus_tag="ACHJ12_004780"
     CDS             627..2078
                     /locus_tag="ACHJ12_004780"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4992444.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2068..3138
                     /locus_tag="ACHJ12_004781"
     CDS             2068..3138
                     /locus_tag="ACHJ12_004781"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4992445.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEDEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3138..4886
                     /locus_tag="ACHJ12_004782"
     CDS             3138..4886
                     /locus_tag="ACHJ12_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4992446.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(4936..>5945)
                     /locus_tag="ACHJ12_004783"
     CDS             complement(4936..>5945)
                     /locus_tag="ACHJ12_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4992447.1"
                     /translation="VAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKP
                     ETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVY
                     GIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQE
                     NYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIEEREGKTF
                     HYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSA
                     GQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEMVDDYNSL
                     R"
BASE COUNT         1385 a         1512 c         1499 g         1549 t
ORIGIN      
        1 cccattaatg attcatgtgg tttatttaaa taaccattat gtgcattact ccgcaaatct
       61 gacctttcac tctgtctcaa attgtcattg tttaccgaca tatcgcctct catcacttaa
      121 tcgctccacc attacaattg attaaaatat attttatgga gtccgattaa tggcagctct
      181 tactgcaagc tgtattgacc tgaatattca gggcaatggc gcttattccg ttctgaagca
      241 gttggcgaca atagcgttac aaaacggttt tatcaccgac tcacaccagt tcctgcaaac
      301 cctgctgctg cgcgaaaaaa tgcactctac tggatttggt tccggtgtcg ccgtgccgca
      361 cggtaaaagc gcctgcgtta aacaaccgtt cgtattattc gcccgcaaag cgcaggctat
      421 tgactggaaa gccagcgatg gcgaagacgt caattgctgg atctgcctcg gcgtgccgca
      481 aagcggcgaa gaggatcagg tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca
      541 ggattttatt catcaactgc aacagggcga cgccgaccag gtgcttgcct tgttaaatca
      601 aaccctcagc tcataaggaa gtggcgatgg agtcatcctt acgtattgtc gcgatcacca
      661 actgccccgc cgggatcgct cacacctaca tggtggcgga agccctggaa cagaaagccc
      721 gttctctcgg ccataccata aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct
      781 tatccagcga agagattgcc gctgccgatt acgtcattct cgctaccggg cgtggcctga
      841 gcggtgatga tcgcgcgcgg tttgccggga agaaagttta tgagattgcc atctcccagg
      901 cgttgaaaaa tatcgaccag attttcagcg aattaccgac aaactcgcag ctttttgccg
      961 cagatagcgg cgtgaagctt ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc
     1021 tgatggcggg cgtctctgcg gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc
     1081 ttgccaacat gctggtgcag ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt
     1141 cgtttacctg ggtagttgaa tccatcggtt accttggctt cacctttatg atccccatca
     1201 tgggcgctta tattgcctcg tcgattgccg ataaaccggc tttcgctccg gcgtttctgg
     1261 tttgctatct ggcgaacgac aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg
     1321 gcgcggtggt gctggggctg gcgattggct atttcgtctt ctggttccgc aaagtgcgcc
     1381 ttggcaaagc attacaaccg ctgctcggtt caatgctgat cccgttcgtt actctgctgg
     1441 ttttcggcgt gctcacttac tacgttatcg ggccggtgat gtccgacctc atgggcgggc
     1501 tgctccactt cctgaatacc attcctccat caatgaagtt tgcggcagcg tttctggtcg
     1561 gtgcgatgct ggcgttcgat atgggcggcc ccatcaataa aaccgcctgg ttcttctgct
     1621 tctcactgct ggaaaaacac atttatgact ggtacgccat cgtcggcgtt gtcgcgttga
     1681 tgccgcccgt tgcggcgggt cttgcgacgt tcattgcgcc caaactgttt acccgacagg
     1741 aaaaagaggc tgccagtagt gccattgtgg tcggtgccac agtcgcaacc gaacctgcta
     1801 ttccttacgc tctggcagct ccgttaccga tgattaccgc caatacgctg gcgggcggta
     1861 ttaccggcgt gctggtgatc gctttcggaa tcaaacgtct ggcacctggt ttgggtatct
     1921 ttgacccgct gatcggcctg atgtcgccgg taggttcgtt ttatctggtg ctggcgattg
     1981 gcctcgcgct aaacatttca ttcattatcg tcctgaaagg attgtggtta cgccgcaaag
     2041 cgaaagccgc gcaacaggag cttgtccatg aacattgagt tactgcaaca gttgtgcgaa
     2101 gccagcgccg tcagcggcga tgaacaggaa gttcgcgaca tcctgataaa cacgctggaa
     2161 ccttgcgtta atgaaatcac ctttgatggt ctgggcagct ttgttgcccg taaggggaat
     2221 aaaggtccaa aagttgccgt tgtcgggcat atggatgaag tcggctttat ggtcacccac
     2281 atcgacgaga gcggttttct gcgcttcacc accattggcg gctggtggaa tcagtcgatg
     2341 ctcaaccacc gggtaaccat acgcacacac aagggattta aaatccctgg tgtgattggt
     2401 tccgtcgcgc ctcatgcgtt aacggaaaag caaaagcaac aaccgctgtc atttgatgag
     2461 atgttcattg atattggcgc gaacagtcgc gaagaagcgg aaaagcgcgg cgttgaaatt
     2521 ggcgatttta ttagcccgga agccaatttt gcctgctggg gcgaagataa agtagtcgga
     2581 aaggcgctgg ataaccgcat cggctgcgcg atgatggcgg agctactaca gacagtaaat
     2641 aacccagaaa ttacgctgta cggcgttggc agtgtggaag atgaagttgg gctacgcggg
     2701 gcacaaacct cggcggaaca cattaaaccg gatgtggtga ttgtgctgga taccgctgtc
     2761 gcaggtgatg ttccgggcat tgataacatt aaatacccgc tgaaactggg ccaggggccg
     2821 gggctgatgc tgtttgacaa gcgctacttc cccaaccaga aactggtagc agtgttaaaa
     2881 aactgtgccg cacataacga tttaccgctg caattttcca ccatgaaaac cggagcgacg
     2941 gatggcgggc gctacaacgt gatgggcggc gggcgtccgg ttgtcgcgct gtgtctgcca
     3001 actcgttatc tgcacgctaa cagcggcatg atttcaaaag ccgattacga tgctctgctc
     3061 acgctgatac gggattttct gacgacctta actgcggaga aagtcaacgc gtttagccag
     3121 ttccgtcagg tggattaatg ctaaacgaac gccagttaaa gattgtcgat ctgctggagc
     3181 aacagccgcg cacgcctgga gagctggcgc aacagactgg cgtttcaggc aggaccatcc
     3241 tgcgtgatat tgactatctc aacttcaccc ttaacggcaa agcccgcatt ttcgccagtg
     3301 gtagtgcggg ctatcagctg gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa
     3361 agcacgataa cgacgatcgg ctgctggcgc tattattact aaataccttt actccccgtg
     3421 cgcaactcgc ctcggcgctt aatttgccag aaacgtgggt agcagaacgt ctgccccggt
     3481 taaaacagcg ttatgaacgc acttgttgcc tggccagtcg ccccggtttg ggccatttca
     3541 ttgatgaaac agaagagaag cgcgttatct tgctggcgaa cttgctacgc aaagatccgt
     3601 ttttaattcc gctggcgggc ataacacgag acaaccttca gcatttatcc acggcctgcg
     3661 acaaccaaca ccgctggccg ctgatgcagg gtgattatct ctccagcctg attctggcga
     3721 tttacgccct gcgtaatcag cttactgaca tatggccgca atatcccggt aacgagataa
     3781 aacaaatcgt tgaacagtgc ggcctgtttc tcggtgataa cgctgtaaga accctgacgg
     3841 gtttgataga gaaacagcat cagcaagcgc aggtaatttc agccgatcat gtgctggggt
     3901 tgctgcaaag ggtgccaggc atcgcgtcat tgaatattat tgatacgcag ttggttgaga
     3961 atattaccgg gcatttatta cgttgccttg ccgcaccggt gtggattgct gagcaccgcc
     4021 aaagcagcat gaataacctg aaagccgcct ggcctgcggc ctttgatatg agtctgcact
     4081 ttattacgct actgcgtgaa cagctcgata ttcccctttt cgacagcgat ctgatcggtt
     4141 tgtattttgc ctgtgcgctg gaacggcatc aaaacgaacg ccagccgatc attttgctct
     4201 cggaccagaa cgcgattgcc actattaatc agctcgccat tgagcgcgat gttttaaatt
     4261 gtcgggtaat tattgcccgt agcttaagcg aacttgttgc cattcgcgaa gagattgagc
     4321 cgttattgat cattaacaat agccattatt tactggaaga cgcggtaaat aattacatca
     4381 ccgtaaaaaa tatcattacg gctgccggta tcgaacaaat aaaacatttt ctggcgacgg
     4441 catttattcg ccagcaaccg gagcgttttt tctctgcccc cggaagtttt cattattcga
     4501 atgtacgcgg tgaaagctgg caacatatta cccggcaaat ttgtgcgcaa ttagtggcac
     4561 aacaccatat taccgccgat gaagcacaac gcatcatcgc ccgcgaaggc gaaggtgaaa
     4621 acctgattgt taatcgcctc gccatcccac attgctggag cgaacaggag cgacgttttc
     4681 gtggattttt tattaccctc gcccacccga tcgaggtgaa taacgaaatc attaaccatg
     4741 tgttgattgc ctgcgccgcc gccgatgcgc gtcatgagct aaaaatattt agctatctgg
     4801 caagcgtatt gtgtcagcat ccggcagaga ttattgccgg gttaacagga tatgaggcat
     4861 ttatggagtt acttcacaag gggtgaactg tgtcggatgc gccgcatccg acactgcatt
     4921 tatcatcaaa aacgtttaac gcaggctatt atagtcatca accatttcgt tgtactcgcg
     4981 taatgctctc gggaaagagt cgtcgaccat ccagcttgag tttgcgtcct gcagttgctc
     5041 tttatcccag tcagagtacg ggactttatc tctgatgcgg cgaacgtatt ttttagcctc
     5101 gagttgatac tggcctgccg aattaataaa cgagaaattc atgccgcctt tatccgcttc
     5161 tttggcctgc gccactagcg tttccagttc agacactttc ttcattgccg catcaacatc
     5221 aaacttctct tgcgatatca gattattaat ttgtttcgct gaaatcatga ctgccagaga
     5281 gtaatagtgg aatgttttcc cttctctttc ttcaatattt ttcagttccg caagctggcg
     5341 cttatcgtta atttcctgaa tcgccgcatg ataagattct gctacagctt caaaggcttc
     5401 cagatttttt aagaatgtct ggtgcagcgt tttccctttc gcaaacgcat cgtctttata
     5461 attttcctgg gtgtaatatt tatccatttc gttaatggta ttacccaacg ccacggcagc
     5521 atcgatataa ctcaccgcca cgccatcaat aggttgcagc gcaggcgtta acgccacagc
     5581 actttttacg cctttttcac actcagcgag gttggattca ctaatgccgt agatgccata
     5641 aacagtacgc tcttcaccgg taggcccctg tttaaaatct ttcagccagt cagcataacg
     5701 cgccagactg ctctgtacag ggatctgtaa cttgttatag cacttgatat agacatccat
     5761 tttttcgtcg aaaaccgctt gttcatcgac caccggttgc gcaggtgttt caggtttagc
     5821 ttctgctttt gcaacgggag cttcaggagc tggtgcggca ggttgactat tggcaggcgg
     5881 gggcgtttct gtttcggctt ttttatcatc acaacccgtc agaccgaggg ccatgatggc
     5941 gacta
//
LOCUS       DAZFFI010000196         5920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFFI010000196 DAZFFI010000000
VERSION     DAZFFI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.198"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHJ12_004784"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHJ12_004784"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4992448.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(858..1694)
                     /gene="kduI"
                     /locus_tag="ACHJ12_004785"
     CDS             complement(858..1694)
                     /gene="kduI"
                     /locus_tag="ACHJ12_004785"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4992449.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1981..3162)
                     /gene="yqeF"
                     /locus_tag="ACHJ12_004786"
     CDS             complement(1981..3162)
                     /gene="yqeF"
                     /locus_tag="ACHJ12_004786"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4992450.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTSVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3417..4646
                     /gene="yqeG"
                     /locus_tag="ACHJ12_004787"
     CDS             3417..4646
                     /gene="yqeG"
                     /locus_tag="ACHJ12_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4992451.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5106..5738
                     /gene="yqeH"
                     /locus_tag="ACHJ12_004788"
     CDS             5106..5738
                     /gene="yqeH"
                     /locus_tag="ACHJ12_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4992452.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPCLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1632 a         1416 c         1253 g         1619 t
ORIGIN      
        1 ttatgatgat ctacagatat aaaaaaccct gccatgcggc agggtcataa aagtaagaag
       61 aatgaattaa cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc
      121 tgaagcgctg gaggcaagga acactaccgg ccccatcagg tcactcggca gtccccaacg
      181 gccagctgga atgcggtcga ggatttccgc gctacgttgt tcatctgccc gtagttgttg
      241 agtattgttg gtcgccatgt aacccggggc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcgttc gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
      421 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg
      541 aatcaatccg gcgttattca ccaggatatc aatatgacca aactccgcta ccgcgcgatc
      601 cagcagtgct ggaataccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct tgatggtttc agtcggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacagcc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag
      901 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
      961 tgaatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat atgacgcgtt
     1021 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
     1081 taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacccgga
     1141 gccagctccg tcagccccat actcaattgg caggtttcca gtacatccgg gacaaaatat
     1201 ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
     1261 tccggtgtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac
     1321 ttcgccggag tgccggtatc aatactggca aagacaactt cttttgcacc tttaccaaca
     1381 tacagggcgt cgcggtgacc gatttcatag cattggccat cgacagtaat cgtaccggca
     1441 ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt
     1501 ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata
     1561 attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc
     1621 aaaaattcat tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcact gtggatgctc
     1681 tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt
     1741 gaaacactgt ttcgttttct ctataaaggg atgatatgcc tatgggctag ggttttcaat
     1801 taaaaataaa acaatgtttc atttttaagt taaggattaa aaaaagtgca gtgtcgatca
     1861 cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag
     1921 ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag
     1981 ctattcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc
     2041 cagtcctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca
     2101 accggaagcg cctatcgggt gaccaagtgc gatcgcacca ccattgacat tgacccgacg
     2161 ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc
     2221 ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc
     2281 atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcgaat
     2341 gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc
     2401 agctgcgcca tcgtttatgg atgatgcatt acccgctgtc acagaaccga gactatcaaa
     2461 tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt
     2521 atcaacaacc aacgtctgac cgttactttg ggtcattacc gggacgatct catctttaaa
     2581 tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg
     2641 caattgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata
     2701 atcattgaag gcatcccata acccatcatg cacaagactg tcaaccagct ggctattgcc
     2761 aagctgtgca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg
     2821 gccaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag
     2881 tgctttaagt ccggaaccgc agacgtcatt aatcgtgatt gcagaaacgc tattaggcag
     2941 accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg
     3001 accaagaatt acttcatcca ccgcatatgc aggaacgctg gtacgttcta ttaacgcttt
     3061 cacgaccaga ctaccaagtt ccacggcgga atgacccgct aacgcaccgc gaaagcagcc
     3121 gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt
     3181 aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat
     3241 ttttatcaca aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt
     3301 tgcaaccact aacccaccaa tagaggggta gatagggcgt taatctccca tacttaacct
     3361 ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt
     3421 ctaatatttg gtctaaagaa gaaactctgt ggagtttcgc gctctacggc acagccgttg
     3481 gtgcaggcac gctcttcctt cctattcagt taggttcggc aggggctgtg gtcctgttta
     3541 ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca
     3601 tcctctcatc gaaaacatca gcaggtgaag ggataacggg cgcagtaaca cactactatg
     3661 gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt
     3721 tgatatatgc agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta
     3781 ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaatcctg aatctcattt
     3841 ttctgatggg acgccatgca accattcggg taatgggttt tttggtattc ccattgattg
     3901 cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacga
     3961 cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg
     4021 ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat
     4081 atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct
     4141 gcataagtgt actgttcttt gtctttagct gcctgctttc tattccacct tcgtatattg
     4201 aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc
     4261 cagcatggct gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc
     4321 tgggtacgta ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca acactacagc
     4381 aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg ttggtatcgc
     4441 tgatcacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca
     4501 gcggcccgct cattgccatg atacttttca tcatgcctac gttgtcaacg tatctgatcc
     4561 cggcgcttaa accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt
     4621 gcgtatcggt aatgttcttt agctgatatc cattgtgaat ggttcaaaat tgctacaatt
     4681 ttgaaccatt ccagttttca taatgttcat tcgttattat tccgcactat cacttccctc
     4741 atcatatcct agtattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa
     4801 gactatttaa gatgcataac ttagattcgc aagatatctt ccggtcattc aaaaacaatt
     4861 tgcgatatag atattctcat accatgagat agttatctga accaccctca accaggtaag
     4921 attaaatcag tgaaacataa tcatatttaa taacgagaaa tgcattttaa aatgtcttaa
     4981 tcagaattat agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcatg
     5041 gtgtattgac atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc
     5101 ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt
     5161 gccaaataat gccagaagag tcaccgtgtt tgcagtactg cgcaaactcg tgtttttgta
     5221 tgtggcccga agaaagttca tattttaatc gtggtgtggt agaaggtatc ttaacaaaaa
     5281 accacaatgc gagattaagc ggatatattt ttgtcgattt ttcagtaagt tttttacgtc
     5341 tattccttga aaaagactgg attgactatc ttgcaagtac tgacatgggg attgttttag
     5401 tcagcgaccg caatatgcaa tcgttagcta attactggcg aaaacacaac tcagcaatat
     5461 ctgctgtcat atataatgat gatggtcttg atgttgccaa tgaaaagatc agacaactat
     5521 ttattggtcg ttatttatca tttaccagag gtaacacatt aactcagatg gaattcacca
     5581 taatggggta tatggtttct ggttataatc catatcagat tgctgaagtt ctggatatgg
     5641 atatccgtag catctacgcg tacaagcaac gaatcgaaaa gagaatgggt ggtaaaataa
     5701 acgaattatt tattcgttca cattcggtcc aacattgata ctcaacgacc agccagaatc
     5761 atactctgtt tatacgggca tttattgaaa tgtccgtatt ttataaatag gccgtcccct
     5821 caagagtaaa caccatagta tttaaaacca tcacacgaaa aattcagaag cattacgaaa
     5881 tatgaattta caaacaatag tggcataaat gttaaccatg
//
LOCUS       DAZFFI010000197         5895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFFI010000197 DAZFFI010000000
VERSION     DAZFFI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.199"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..4784)
                     /locus_tag="ACHJ12_004789"
     CDS             complement(<1..4784)
                     /locus_tag="ACHJ12_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFO4992453.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGELAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAVIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPNDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHCYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVFRHSNRGFACEQGWNAASQLVTQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRERQAHPSVAPLWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEAAPGLLYAGQWQDTESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNL"
     gene            complement(4804..5265)
                     /locus_tag="ACHJ12_004790"
     CDS             complement(4804..5265)
                     /locus_tag="ACHJ12_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4992454.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5293..>5895)
                     /locus_tag="ACHJ12_004791"
     CDS             complement(5293..>5895)
                     /locus_tag="ACHJ12_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992455.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT         1068 a         1812 c         1583 g         1432 t
ORIGIN      
        1 aataaatttt tactttttaa tatttcattt agaacgtgtt cctccctaga taaacgtcct
       61 agtgcttcgt atgcttctcg tccaattttc gcaaattgct tagcatgaat cacttcatga
      121 tacacctcat ataaggtagc atttttccta agataaatat tgcgaaggcc ataatcaaaa
      181 gctgccctag catttggtgg caaatattta tctgctttcc ttattacttt aattcctagc
      241 ttctccattt ctttagcaat attgtttatt tgactacgtg ataaacgaac cccatccagg
      301 aaacccgtat gcccactaac acgacgacca tcgccaatat agtccatcaa actgctgatt
      361 ttagaacttg cagccaatcc cagcggatca acccacccac aaggattcgg cacataccgg
      421 taagtctgct ccccgcccag caaccccagc ggatcactca ccaggtacat tccggtttcc
      481 ggctcgtaat accggaaccg gttatagcac agcccggatt ccgtatcctg ccactgtccc
      541 gcatacagca ggccgggggc ggcttccggg tccagttccc cgcgcgggtc cgggtagccg
      601 gtgtctgcgg gcaggctgag tgccagcccc cacaggctgg tctgtcccgg ccgccagacg
      661 gttttacctt cactgttaaa gagcatcagc gggcggcccg tcaggtcgct caccgcatga
      721 ttcgtccgcg tcacccacag cggggccacg gacggatgcg cctgccgttc ccggctgaac
      781 tgctggctta tcagctcaaa gccgttaaac accaggtgcc gtacgctgta cagtttatca
      841 tcgcggtatt cccggatttc cgcaatactg tcgccgtccc acaggtacgt gaagcggatt
      901 ttcttccggt cgcagcgttt ctctgttcgt ctgccgctgg cgtcgtggcg gtattcccac
      961 agctccccct gtgcgctgca atacccggtc agctggtccc ggctgtccca ccggaaccgc
     1021 tctgtttccg ggcggtagcc gtcacggtgt tttgtccggc tgaccatccg gcctgcggcg
     1081 tcatagtcat actgcgtgtt tccggcctgt ttcagccggt ggcctccggc atactggtca
     1141 gtctcttcac ttatccggtg cctgcccgca gactgcgcct tcaggtagcc acaactgtca
     1201 tagccatagg actcatcgcc ttcgccatag cccagcccgg tgcctgaggc tgtcaccgcc
     1261 gtgacctgac cgttccggtc cagccggtat tcccgccgtg tttcccgtcc gtaatcttcc
     1321 cggctggtga cgcctgacac gttacccgcg ctgtcgtaac ggtactcccg taccagtgag
     1381 gggagcagcc cgccccatgt ggtttcctcc gggaaaaatc cggcgcgctg ggtgacaagc
     1441 tggctggctg cattccagcc ctgctcacag gcaaaacccc ggttactgtg ccggaacact
     1501 tcacgtcccg cctcatcacg gctgaacgtc agcgtctcat cccccggcag aatgacctcc
     1561 gtcagttctc cggcggcgtt ataacggtac gtggttttca gcagggattc atcggggtgt
     1621 ctgtcgcgtt catcttccgg tggcaggata cagcggataa cggtgtgttc atcggggtag
     1681 tggtattcaa tggcccgtcg gccctgagtg gtgacattca cccgcccgcg ttcgtcgtag
     1741 ccggtgcgct gtgatgtgcc gtccgggctg aagatacccg tcagcctgcc ggatttgtca
     1801 taggcatact gtgttttccc ttccggcgag atgacttctg ccaggcggcc tgcggcgtcc
     1861 cggctgtaga ggatggtttc ctccaggccg ttgaccctgg cggtacacag gccatcagcg
     1921 tcatagcggt aacgccagac cacgccgccc cagtcacgct cttccgtcac ccggccacag
     1981 ttgtccagaa tataacggta tgactcgccc tgcgggttaa tgacttctgt gagctgcagc
     2041 gtgtctttat cgtagcggta ctgccagcgg tggccttcac cgtccgttcg cgccaccggc
     2101 aaatcgaacg ggccgtattc cattgtccag acaacgccgt taccgtccgt gtacgccagc
     2161 aggttgccgt ggcggtcata gcggaatcct tccgcgccgg ttccagcacg ttcaatgccg
     2221 ctcagtttat ggtgctgctg ccactgtaac cgcactgact gaccgccggg gccgcgcatc
     2281 ttcaccggca ggtcgtgacc actgtattcg gtgtggtgtt cacggcccat cacgtcggtg
     2341 agtgtttccg gccggttgtg gtggtcgtac tctatgccca gacgccgttc atcctggcgg
     2401 aatatccccg acagcagccc ctgcggcgtg tacgccagcc gggtctcttc gccctgtggc
     2461 gacaccagac ttaccggcag gccgcgttca tcacactgct gtgtccacgt ctgcccggct
     2521 gggtcggtga tgttgctgag caggccgcgc tcatcatagc ggtacagcca ctccgcgccg
     2581 tctgccgccg tgtaacggct taactgaccg agtgcggcgt tgtattcaaa cttctccacc
     2641 cgcccggcag gggagtgacg ggccagcagg tgatggaatt catcccattc atacgtggtg
     2701 gtgctgccgt ccggcgcttc ttcccgcaga atattgccgt caggatcata gtaataacag
     2761 tgaatgccct tgccgtccgt cacggtggtg cagccgtcac catagtccag cgtggcgttg
     2821 taatagccct ccgccccggt gacggtcacg caccggccct gtgcatcgta ggtgaaacgg
     2881 ctccacgtct ggtcgttatc ggaccagcgg atgatccggt ccgcagcgtc gtactcataa
     2941 aacaggtggt agtccagccg cgcatccgct tctgtcagcc gcccccgcgc atcctgttcg
     3001 taggtcgcca gcgtctgtat gccgttatcc gtgcggtgaa tggctttcag gtagccgctg
     3061 gcgtggtacg tcagtttaag ccggatcccg tcggtgtgac tgacacccgt gatccggtac
     3121 tggctgtcgc gctcaaacgt gatgcggttg ctggcggtgt cgctcatccc gcccagcatg
     3181 tacatccggt cattggggct gtgtgccagg cgatcatcgc tggtatcgtc agtcaccggg
     3241 tggcgcagac gcattcccgg aaaggcgtca taaaagaagc ggctgctcag tgcatccctg
     3301 tcgaacagct ccagataatc atcacggcgg cgcagaatgt actgggcgtg atacgggtta
     3361 accgccgtat cactttcacc gtcgaaggcg aagttcagcg tggctccctg gctgatgata
     3421 tccacccggt ttccctgttc acgcacccag gcgtattcgc tccaggagtc gctccagccc
     3481 acgcccgtca ggccatgcag cggcagtaca gaactgtgga aacgggtgaa ggagaggggg
     3541 agggtctggc ccagggtgaa gtcggtacgt tcgtcgcagt atgcgccggt taccggatcg
     3601 acaggatctg ttacccatct gatgacttta ttggcaatac tttttaacga cttacctgtt
     3661 ttccccagca acgcgccgag ctttgcgccc agcagcttcc cgcttttccc cagcaggttt
     3721 cccagtttgc cgagtctcgc cagattcttc agttttccca gcagtcccgc aggcggcaga
     3781 agatagctgg ctatcgttat tcctcccagg atcattctct gccacggcgg gaactccggc
     3841 tggatatcca gatatgtgac ctgctcaccg ccaataaaga cgtccggcga acctcccacg
     3901 atcaccgcac tgcactccag tttgtcgccc ttgcgcgcgg caggctgatt gttgataaag
     3961 acgttgctgc tgccctgggc tatcctcggt ggtttgttct ccttgtcgca gagcgccgtg
     4021 tcctcaccgg cttccgggaa atccacctct tttttgattt cgccacgccc gcccaccatg
     4081 gtggcaaact ccattaatac cggaccggcg ggaaacagaa gcgatgcgcc ggcgctgacg
     4141 cgatccataa aaccggcatt gctgtccgtc agggtgataa cagcgttccc ccaggccttc
     4201 gccagtggta ctgctttttc cgctgccgat tcaagcaggt cagtggcaat atcaccccat
     4261 gagggttccc cctgtgggac ttcaggtatt gctccaggtg ggggaggagg gggaagggtg
     4321 accgctgcac gtgcggccag ctcgccgtta atttttacgt tttcagaacc ggtattgatg
     4381 gcgccgtccg tgtcacaaaa gctgtttatc catgattcaa gctggttctg tttctcctcc
     4441 acataatcac tcgccttata gctggcatac atcagcgcag gcaccagaac cagcgcataa
     4501 gggccggtgg cacaggcggc cataatcagg caaccttctg cgatggtcac tatcgccccc
     4561 agcacggccc ctgccagcgc ccccaaaaaa ctcttgtgct gaatttcatc gcccagcctg
     4621 gcggcggggg ggccggactt ttttgatgcc aacatctgtg caatggcttt gtcgttggct
     4681 gcccgtttca tctggctggc gaccatcgcc tgctgtgaca gcattcgcat tgccagcgta
     4741 ccgcctgcgg tcgctccctg tggcccgcct ggtccttcac tcatgtgtta tccctcgttt
     4801 atgtcagcgc ggctggtcct tttggatgtc attctccggt ggcaacgggg tgaagctctt
     4861 tatcacctcc agcagcatcg tcttttgact gttcttcagg gataaggagg tgctgaaagt
     4921 gaaggtcagc agcgtatctc cacgccgctg gaatacggct atctggctaa tccgcccctg
     4981 attcccctca tcttccggac cgttccagga ataatccagc agccaggccg gggcctctgc
     5041 cagagtgatt tcccggcagg catgttcggt gtacccatca agtttgccct ggtacagcgc
     5101 cagcgtttcc tggtacagct ctggtgtcag ggttccctgt ggaacggctt cgcgggtaat
     5161 gaccaggttg atgccactgt tatctgtgga caccaggacc tgcattgact gatcctgcca
     5221 ttgagacggc agttgaatgt ggccttcatt aaaagtgaac tgcataaaat actccctgat
     5281 aatctgagtg aattagttga tgaaaacaga accgcccacg atggtgatgc catccttcga
     5341 cagcctgatt accgtcgatt ccccgacctg tagctgaatg ccatccggcg tcagcgcaat
     5401 tttgctttcc tccacggcca gcaaaatttc cttaatcgcc gtataagcct gtataccgtc
     5461 gccttcgcca ccattgttta cagcgtcaac atgcaccgaa ccggcaatcg tgcctctgtc
     5521 ctgaaagaca tcctcagata aagacccttt cagtactccg gtgatcttgt ggcctttttc
     5581 caccgtaaga aggcggttac cttcctggat cgtgaccgtc tggtcatttt ttacggtttc
     5641 gcgatgatcg tgttttacgg tggtggtacg gtcgttgagc acttcggtat ccatgttctt
     5701 ctgcgcgtgg atgtagacct gttctctccc tgtcgcatca tcaaacttca gctcattaaa
     5761 tccgctgccc atgtacgttt tggagcggat ggtcatctgc gtctttgttc ccggcaggct
     5821 gccgggggtg cggttttcct ggtggtaggt gcgccccata atgatcggct ggtccggatc
     5881 gccgttgagg aagtc
//
LOCUS       DAZFFI010000198         5778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFFI010000198 DAZFFI010000000
VERSION     DAZFFI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.200"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..132
                     /locus_tag="ACHJ12_004792"
     CDS             <1..132
                     /locus_tag="ACHJ12_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4992456.1"
                     /translation="AFGASDHQIIHVTAWTEREARNRCPSGCVAVFAARIRQEVRHV"
     gene            125..343
                     /locus_tag="ACHJ12_004793"
     CDS             125..343
                     /locus_tag="ACHJ12_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992457.1"
                     /translation="MFEVLIVITRYHNEAVMSRREINTYRYKSRRTAVKKFGQIAEEI
                     KNDLFRVRQVKGSPCKYVSSVELRYVAE"
     gene            330..563
                     /locus_tag="ACHJ12_004794"
     CDS             330..563
                     /locus_tag="ACHJ12_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992458.1"
                     /translation="MLQNKTTPDAAEEAMQILIRALVDVTHMVESMERKSQSEHDKRK
                     LKTIKIIAKNSLIKVTDLLNEDIKRIREKICDA"
     gene            556..783
                     /locus_tag="ACHJ12_004795"
     CDS             556..783
                     /locus_tag="ACHJ12_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992459.1"
                     /translation="MHKIPFEVLIHSENALIRAKEMNALLGKLIEVPESGEESSSLMF
                     AAIQTLLSPVIDELDKAMDIIENNKAPHTGE"
     gene            789..1088
                     /locus_tag="ACHJ12_004796"
     CDS             789..1088
                     /locus_tag="ACHJ12_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4992460.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKQ"
     gene            1085..2494
                     /locus_tag="ACHJ12_004797"
     CDS             1085..2494
                     /locus_tag="ACHJ12_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4992461.1"
                     /translation="MKLAPNVKLLPKDKYTDAIIFAGIDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLAIAGVQEARLL
                     SENFELLEEWSDQLPRLREAWERGESLVMKKIPQRKTTLPMSVGSTGYDTQLDYVVKG
                     IIPASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHGEQVKNLYLVNRPIFPAVPLDVDEMVIAARQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDA
                     EYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPR
                     PPVELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWL
                     AKLEKDAVIYIDGDDVGPL"
     gene            2697..2954
                     /locus_tag="ACHJ12_004798"
     CDS             2697..2954
                     /locus_tag="ACHJ12_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992462.1"
                     /translation="MTKMRRDRTEPKYKALDMTEHALKVAIRVIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGESGDDT"
     gene            2944..3216
                     /locus_tag="ACHJ12_004799"
     CDS             2944..3216
                     /locus_tag="ACHJ12_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4992463.1"
                     /translation="MIHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWCNYQDS"
     gene            3675..3869
                     /locus_tag="ACHJ12_004800"
     CDS             3675..3869
                     /locus_tag="ACHJ12_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992464.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGAWL"
     gene            3866..5755
                     /locus_tag="ACHJ12_004801"
     CDS             3866..5755
                     /locus_tag="ACHJ12_004801"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4992465.1"
                     /translation="MKTWFSIKAMADVVHVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITSMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNV
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT         1698 a         1276 c         1557 g         1247 t
ORIGIN      
        1 gcctttggcg cgtcagacca tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg
       61 cgtaaccgtt gcccgtcagg ttgtgttgct gtattcgccg cccgcattcg tcaggaggtg
      121 cgccatgttt gaagtgctga tagtgataac cagatatcac aatgaagcgg taatgagcag
      181 aagggagata aacacttacc gctacaagag tcgccgtaca gcggtaaaaa agtttgggca
      241 aatagcggaa gagattaaaa acgatctctt ccgtgtgcga caggtaaaag gaagcccgtg
      301 taaatacgta agttcagtgg agttaagata tgttgcagaa taaaaccaca ccggacgcag
      361 cagaagaagc aatgcaaata ctgatacgtg cgctcgttga tgttacccac atggtggaga
      421 gcatggagag aaaatcacag tcggagcacg acaaaagaaa acttaaaaca ataaaaataa
      481 ttgccaaaaa ctcgctgata aaagttacag acctccttaa tgaggacatt aaaagaataa
      541 gggagaaaat ttgtgatgca taaaatccct tttgaagtat taatccactc tgaaaacgca
      601 ttaatccgcg caaaggaaat gaatgcgttg ttaggcaaat taattgaagt gccggaatct
      661 ggcgaagaat cttcgtcatt aatgtttgca gccattcaga cattactatc gcctgttatt
      721 gatgaactgg ataaggcaat ggatattatc gaaaataata aagcgcccca caccggagaa
      781 taaaaataat gaaacttaaa tattctggct taactgccag tggcaacact caccctaaat
      841 ttacgcgcgg tgatatttac cgcgaccagt acggcggcac ggtaatgatt aagggcgtgg
      901 cgggacggtg cgtaacttac cgccgtgaag gttacgaata tgattgcgtg atgcctgttt
      961 atcagttccg gcgtgatttt tctctggtac agaccgcgcc gcataacgtg cccaccagca
     1021 acgccagggc acgggcaaac atccagaagc tgaaaaccat gattaacgga ttcaggggta
     1081 aacaatgaaa ctggcaccga acgtaaaact attaccgaaa gacaaataca ccgatgcgat
     1141 tatttttgcg ggtattgatg ctcactcatt cgcagagcat tacatcattg cacaggccaa
     1201 aaaagcaggc gatccagtcc ctcccgttta tctggggcgt tatcagttaa gcgaactgga
     1261 taacctccag attgttgatg atgggcgata cagggcgacg gtgatacgcg ccggaaatat
     1321 tgaagagccg cagctgttaa ccatcgccac gaaactggcg atcgccggag tccaggaggc
     1381 gcggctactt tctgaaaatt tcgaattgct ggaggaatgg agcgaccagc ttccacggct
     1441 tagggaggca tgggaacgcg gggaaagcct ggtcatgaaa aaaatccccc agcgaaaaac
     1501 cacgctaccg atgagcgttg gatctaccgg atacgacaca cagcttgatt acgtggtaaa
     1561 ggggattatt ccggcatcgt cgctttgcag catatacggg gcgagcggtt cctataaatc
     1621 attccttgcc ggatcgtggg cgtgtcacgt ttccacgggc cgccagtggg gaggccgcag
     1681 ggtggcgcat ggcgctgttc tctatgtggt tggtgaaggc ggtatcggcg ttccgcgtcg
     1741 tgtaaaagcc tgggaggttg tgcacggtga gcaggtgaaa aatctgtatc tggtaaaccg
     1801 tcccatcttt ccggcggtcc cgcttgatgt tgatgaaatg gttatcgctg cccgtcaggt
     1861 ggagcgggaa acgggtaaac ctgtacgcat gattattctg gatacgctgg cgcgttgctt
     1921 tggtgggaat gatgaaaatg attcccgtga tatgggggcg tttatccgtg gttgtgacga
     1981 actgaaacga cgcacagggg ccacggtgct ggtggttcac cattccggca aggatgagac
     2041 gaaaggcgcg cgcggttcca gtgcatttcg tgcatcactg gatgctgaat accggatacg
     2101 cagggaggac gcaggaagcg aagcgctggt tatctcatgc accaaaatga aggacgcgga
     2161 ggaactcaaa gaagccgcat atgacttacg cgtggtggag ctgtttaccg acgctgacgg
     2221 ggagttaatc acgtcgctgg tggtggtgga taagccgcgc cctcccgttg aactggaacg
     2281 catcgaggag gcaggcaaca agacggaaaa ccataccgcg ctatgggggt gcatccgttc
     2341 acgcacacag aacggcgaca agtgcacgat cccgctgtta cgtgatgaca tgaaacggct
     2401 tgggtatgac gtgaaaaata tgcggcgatg gttagccaaa ctggaaaaag acgccgtgat
     2461 ttacattgat ggtgatgatg taggaccact gtaaaaagtg agtagcaaaa gtgagtaatg
     2521 gtgaggtttt tacaaaatat taacacgatt actcactttc tcacctgtat acatgctcaa
     2581 aagtgaggag taaaaaaatc cttatgaaac acacacataa agcatcaaaa aatatcaagt
     2641 gagacgaagc gagacgcttt aaaaagtgag gtgtaaaagt gagcatgagt gaggagataa
     2701 ccaaaatgcg cagagacaga acagagccga aatataaagc gttagacatg acagagcacg
     2761 ccttaaaggt ggcaatcagg gtaatagacc gccacgcggg ggaaggatac gcgaaggagc
     2821 atcccgaact gataagcgca ttcatgacca cagcggcggc aaactttgcc acgttgacag
     2881 aacgggagat tgccgaagcg gaacaggtaa caaccatcaa cgtaaaaacc ggagagtcag
     2941 gcgatgatac atgaccgcat agcggaggaa ctcgaggcga aaggatttta ccggagggcg
     3001 gcggcgcgat ggggtgaagt catgcagctg gtggagacag acaaggaacg gcatcaggtt
     3061 acgatgcgac ggctggaatg ttccaggaag gcacagaggc caccggagcc gccggataac
     3121 ttcggagacc tgagaaaggc agtagatcgc acttatgctg aaatgggtat agatggtgta
     3181 agcgatgaaa tatggtgtaa ttaccaagac agctaatcac acagccggag caatccggct
     3241 tttttgcggg tcctcctagt ggggtgggcc tgaacacggg gcggaggggc gcggaaaaag
     3301 gcgcattttt tgatttttat ggcaccatca ccaccactat aagttattga tatattgaag
     3361 aataaaaatt tttagtgtcg aatcaggttg ttttttgttc atcactggag cgttcccgaa
     3421 agcatttaca aaaaaacagg cgcaaaaaaa gcgcccccga ttgctgttac cggaggcgct
     3481 tttacacgac aaaggagttt ttatcgccag gatgacgagt cttaatattg cttcaatagc
     3541 aaaaatgcgt caatggcttt gcctctctga gaataatcag aaaaatcata atctgatttt
     3601 atggtagaaa atgatttatc tattactttt atcgatcaat aatgatgccc gttaatcaaa
     3661 acggaggcgg atttatgcca gagaacaaca ccagaaagcc ggataaaagt gccacggtac
     3721 acatagacgc cggaactatg gagaagatcg aacgctatca gcagttcatc aaagataatc
     3781 acccgggtat gccagtgccc acgaaaggac aaatcacacg cagcgcggtt gaatactggt
     3841 acagggcaac gttaggagcc tggctatgaa aacatggttt tccattaagg ctatggcaga
     3901 tgttgtacat gtgcgcattt atgacgagat cggcgggtac ggtgtaaaag catcggcact
     3961 tactgacgag atcaacgcgt gtggtaatgc gtctgaaatc catcttcgca tccattcacc
     4021 tggtggcgac atctttgaag ggctggctat ctataacgct ctgaaaaatc atccggcaaa
     4081 aaaaatcgta cacattgaag gcatggcggc ttctatggcg tcgtttattg ccatgtgtgg
     4141 agatcacatc gttatgcctg aaaacgcgat gatgatgata catgcccccc gtggtgttac
     4201 tgccggagtg tcgggcgacg ttcgccgctt tgctgatctg atggacaagc tgggcgacac
     4261 gatggcagaa acctatgccg gaagaacggg caggagcaaa caggagatca cctcaatgat
     4321 ggaggcggaa acctggatgg atggcaatga gtgtaaggct aacggcttcg cagatgaggt
     4381 tatacccgcg attacagcaa tggcccgaat tgaatcaaaa cgaatcggag atttttcaaa
     4441 tatgccggaa aaaattaaaa gcatgatcag ccagaaaact ggcagtggcg aacaggaacg
     4501 acttaacgga atccgtgaat tgtttggcac cttcaacgga agatataacg acctggctat
     4561 aagttgtctt gcggattcag aatgtagcgt tgagaatgca cgcgaacgcc ttttactcgc
     4621 tatgggtaaa gaatcaacgc caacaaacaa aaccaccccc gcaaatcttt actacgcgta
     4681 cacggataac ggcaacataa ccggcgatgc aatgcgccag gggcttaatg cgcgtcttgg
     4741 tcacgaacgg gccgaacgcg gtaatcctta cgccatgatg agccttttcg atatggcaca
     4801 ggcatcatta acccatcgtg gtataagcac gggcagctac ggcacacgct cgcagatagt
     4861 aaacgtggca ttcacccaca gcagcagcga ttttaccgat attcttgctg gtggcgctga
     4921 aaaatcagtg cttgcaggct gggagcacag cggcgaaaca ttccgccagt ggacgaaaaa
     4981 aggttccctt tcaaacttcc gggaagcccg ccgcgttggt atgaatggct tctcaacgtt
     5041 aaacaaagtg ccggaagggg cagaatataa atacatcacc accagcgatc gcggtgaacc
     5101 catcgcgctg gctacttacg ggaatatttt cagcattacc cgccaggcga taatcaatga
     5161 tgaccttgat cagttatcaa cggtgccaat ggctatgggc cgtgcagcat caagaacggt
     5221 gggaaatctg gttaatctgg tgcttacagg caacgtaaaa ctttctgacg gaatagcgct
     5281 gtttgacaaa aaacacagca acctgattga agcaggactg acaacaccgg gacttagtgc
     5341 agcacgtcac ctgatgcgca cacagaagga caaaaatggc gaagtgctga atattgcgcc
     5401 taaattcctt ttagttccgg cagcactgga agatcgcgcg ttgcagatga ttaactcaac
     5461 cgcacctttc ggggctgata aaaacagcgg gatctttaac ccgtatcaca agctacttga
     5521 tatcatcgtc gatccccgcc ttgatgatat cagcgaaaaa caatggtaca tgctttccgc
     5581 acagggaacg gacacaatcg aggtggctta tcttgatggc aatgacgagc cttaccttga
     5641 acagcaggaa ggttttatcg ttgacggcgt ggcctggaaa gtccgtattg atgcaggtgt
     5701 ggcagctctg gattatcgcg gtatggtcaa atcaggcggg acagattcac tatgacaaca
     5761 aggcggcacc agccgcct
//
LOCUS       DAZFFI010000199         5632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFFI010000199 DAZFFI010000000
VERSION     DAZFFI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.201"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(992..1183)
                     /locus_tag="ACHJ12_004802"
     CDS             complement(992..1183)
                     /locus_tag="ACHJ12_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4992466.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            1248..1571
                     /locus_tag="ACHJ12_004803"
     CDS             1248..1571
                     /locus_tag="ACHJ12_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO4992467.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            1780..2211
                     /locus_tag="ACHJ12_004804"
                     /pseudo
     CDS             1780..2211
                     /locus_tag="ACHJ12_004804"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2249..2779
                     /locus_tag="ACHJ12_004805"
     CDS             2249..2779
                     /locus_tag="ACHJ12_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO4992468.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            2786..3019
                     /locus_tag="ACHJ12_004806"
     CDS             2786..3019
                     /locus_tag="ACHJ12_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO4992469.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            3500..4552
                     /locus_tag="ACHJ12_004807"
     CDS             3500..4552
                     /locus_tag="ACHJ12_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992470.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            4554..5420
                     /locus_tag="ACHJ12_004808"
     CDS             4554..5420
                     /locus_tag="ACHJ12_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4992471.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVI
                     LSWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESV
                     FILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLS
                     AAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGI
                     LQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVL
                     SVYFLNRKYSEE"
BASE COUNT         1487 a         1155 c         1513 g         1477 t
ORIGIN      
        1 aaaaaaaccg ccttgcaggg cggtttgatc gaaggttaag tcagcttgca gactgcttaa
       61 ccaggtaact gtcttatgcg agacgagtca ccaaaattgt tcttctagtg tagccgcact
      121 ccggcagcca cttcaagtac tctctaaata tctcgcacat gacttttacc aatggctgct
      181 gccagtggcg ctttgtggtg tcttttccgg gttggaccca agagaacagt taccggagaa
      241 ggcgcagcag tcgggctgaa cggggggttc ctgcatacag cccagcccgg agcgaactgc
      301 ctaagtcgcc ccgagatacc agaagacgtg agctatgaga aagcgccacg tgtcccgtaa
      361 gggagaaagg cggaaaaggt atccggtaat cgatatggcg ggaacgtggg ggcgcaggag
      421 ggagcgattc accggaaacg gtgatgatct ttaagccacg tcgggtttcg ccaccgctgg
      481 cggatttcat ggttgagccg caaggctccc acagatgcac cgaaaaagtg tctgttttgt
      541 gaagtccgtc aggggggcgg agcctatgga aaaagcgcct ccggcgcggc ctttcccggg
      601 gtttcgtttt ttgcgcacat gttctttcct ggtttatccc cgggttctgt ggataacagg
      661 gttagcgttt ttgagaaagg agagtccgct cgccgcagcc gaacgaccga gcgtagcgag
      721 tcagtgagcg aggaagcgga agagcgcctg aactggcatt ttctccttac gcatctgtgc
      781 ggcatttcac accggacatg gcgcactttt catacaatcc gcactgatgc cgcatggcta
      841 agccagtata cactccgcta tcgctacgtg actgggtcag ggcttcgccc cgacacccca
      901 aaggtcaacg gcctgacgcg ccctggcggg cttgtctgct cccggcatcc gctcacagac
      961 aagctgtgac cgtctccggg agctgcatgt gtcagcggtt ttcaccgtca tccccgaagc
     1021 gtgcgaggca actgcggtaa agctcatcgg cgtggtcgtg aagcgattca cagatgtctg
     1081 cctgttcatc cgcgtccagt tcgttgagct tctccagcag cgttaatgtc tggcctctga
     1141 tgaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     1201 ggggggttct gttcatgggg gtaatgatac cgatgaaacg cgagaggatg ctcacgatac
     1261 gggttactga tgatgaacat gcccggttac tggaacgctg tgagggtaaa caactggcgg
     1321 tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg ttccgggaag ctgccgacac
     1381 tggcaccgcc gttactgcgt cagctggccg ccatcgggaa taaccttaac cagacagcga
     1441 ggaaggtgaa cagcgggcag tggtcttccg gtgaccgggt tcaggtggtg gccgcactga
     1501 tggccatcgg ggatgagctg cgccggctgc gtctggcggt cagggaacag ggggcgcggg
     1561 atgatagtta agtttcatgc caggggaaaa ggtggtggca gtggtccggt tgattacctg
     1621 ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc aggggaatcc ggaagaggtc
     1681 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc
     1741 gcggagaagg agctgccgcc tggagggcgt gaaaaagtga tggcgagctt tgagcgtgta
     1801 ctgatgcccg gtctcgagaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
     1861 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
     1921 cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac gctggtgaat
     1981 caccattacg gtctgcatga ccccaacgcc ccggagaacc gccggacgct gacccttcct
     2041 gataacctgc cggaaacgaa acaggcgctt gctgagggcg tcacacgcgg gatagacgcg
     2101 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg
     2161 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaatggcggg
     2221 aggaatatca gactgaaagg agcgttttat gagcaatctt ttacagacgg gcgcggagtt
     2281 cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg acgagttcag
     2341 gaagctcgaa gaatctgtaa acagggaact gacctcaaac gaatctttaa tcaggaacgc
     2401 tatcaacgac catacaacag ccctgaaaga actgctggag aggtatcaga aaacgaccgt
     2461 cgatacgatg gacgcgcact ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg
     2521 gctgattatc gtctcagtac tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg
     2581 catgaagata aacgcgaata tgaatattct ccgcgagcag aaagagagtc tggagaagct
     2641 gaacgcgaag acatggggcg tgcgttatca cgaggacagc aacggacgtt ttctggtgtt
     2701 gccgaagggg atgaaagccg aaacgaactg gacgaaggac aacggcaaac tgaacgcagt
     2761 gagactggtg caggagtgac agagcatgac ggagctggaa agacatttgc tgaacgcatt
     2821 agagcaactg caacaggact atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga
     2881 attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat
     2941 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata
     3001 cagcgagaac aggagataag ggatgaacgg ctgatgcagg aacgcgaaca tgagttatct
     3061 ctggagcggg agcgtcagcc ggaaatccag gagcggacac tggatggccc ttcgctggga
     3121 tggtgaaacc atgaaaaatg acgtgttcag tggattaagg cgggctaatg tggcctgtag
     3181 catctggttg cataggtatt catgcggtta aaatttatca ggtgcgatcg cggcagtttt
     3241 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcaccga acgcgttttg
     3301 gcggtgcgta caatgcgacg ttatggtaaa tggagcggat tttcaggaag gagtgatact
     3361 aacaactcga acactgtgca aataatgatt tttttaaaat atcggtaacg ataataaatt
     3421 atttcgtttt acgccggtaa taaatataat gtaatttcag tatataaaaa aatatatgag
     3481 gtgtcttgtg ggagctttca tgaatcttca acaacgtata aataaactac cacaattatc
     3541 atccagtttt tcatttggaa aagatattga taatattcat tcatttatct ttaacgaaac
     3601 ttctaaagat aaaattgaag atttactaag aaaatgggtg tcaggcaatc agccctgtgt
     3661 atttggtaaa ttggcaagca agaaaattaa agggcttgat ttccatttat ctattgtaaa
     3721 tagcccccaa ctatataatg atgatggtca tttgtttgat tttttgcgta atgaaagagt
     3781 tcgttttaaa gaacgcgcaa gaagaggaga ggtcagtgcg catctcattt attttattca
     3841 tccacaactt gcctttgctc gtccatcaga agagttagtg gacatacaaa aatatatttg
     3901 ttcgctgcat atgccagagt gctatccaat aaaagaggat gttatttata ctgaaagcgt
     3961 accatttcag gataaagatg ggctaaaaat atataaggct ggtgttaatg ttttttactc
     4021 ttctgctcat agaacaagaa atcatgatag aagaatacca gggggaatac ttatttctgt
     4081 aaatgccccg gggcatttta tgaggctggc gatagaaaaa ggtttctata aagatcaaga
     4141 acaagcgtta gcggatattc gtaacatgac tattcagtct gttggtaagg gtgggtattc
     4201 acatccggaa gggatttcta caacatggca ttcagaatct aatttggatc gttttggatg
     4261 cccagtgcac accggtaata gttcttatta cagcggtttt tatcatactg atgtattaat
     4321 tccaggagaa ttaactaagg atgaaagatt gctccatgaa atagaaaata gtgatccaat
     4381 gatcttcaac tggaatgtat tgttttatgt ttcattagaa gagtttccta tagatgatcc
     4441 ttattatgga gagtttattg gtattcctgt agatgatgca tctatgtttt ttaactcctt
     4501 tcaaccaagg aaatttgaaa acaaacccct ttatgagaaa gaggatgatt aatatgatta
     4561 aaggaatgct ttttgctttt ttagcagcgt tctcatgggg ggcagctatc gtaatgagca
     4621 aaaaaggagt tgaagaacta gatgccggag gggtgttttt ctggcagatt tgtagtgcag
     4681 taatactatc ttggatagtt ctttttataa aaagaaaaaa actcccatta aactcaaaag
     4741 cgatacttgc ttatgcgaca ggcgtttttg aaccattctt agcgtatact tttacactct
     4801 acggacttta ttttatttca gcaggaagcg catcaattat tttttcactt gaatcagtgt
     4861 ttatattggt gttgtctgtt cttatttgct cagttaaaat taattccccg caatatttta
     4921 tattgttcct tataggtgca attgttggca gtattttggt agccttgcca gacatcagta
     4981 atggatcgaa taatattgtt ggatattgtc ttgttgttgc cggtgtgtta tcggcagcat
     5041 tttatgttgt tatatcgtct cgccttatta aatcgtttga gcctatcaca ttattgagcg
     5101 gacagttgac attctccctt atactgtcat cgctctttat ttttatatct aaatcatcaa
     5161 tttatcttcc gttggaatcc gtgattattg ttttttcatc tggaatatta caatattttt
     5221 tagctttttg tttttacttg aactccttgc gttggctccc agttcatttg gcagggataa
     5281 tgttgtactt tattccgcta gttgcattat ttttgtcatg gttgtttttg gatgaacaat
     5341 tcacattctt acagggggta ggatttattg taactgtgct aagtgtatat tttcttaaca
     5401 gaaaatactc agaggagtaa cattctgttc aggattgccc cccggcctcc atcgataccg
     5461 tgccaaatgc acgtaaaaaa cgggagcgtt tctggtgtcg gaatgtaacc aagggggtca
     5521 gaggcgcgct aaaacgctct caggggagtc aaatagagac gtttgtaaca aaaagatcaa
     5581 aggatcctct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cg
//
LOCUS       DAZFFI010000200         5604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFFI010000200 DAZFFI010000000
VERSION     DAZFFI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.202"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(16..417)
                     /locus_tag="ACHJ12_004809"
     CDS             complement(16..417)
                     /locus_tag="ACHJ12_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992472.1"
                     /translation="MDKENCSISERWLIASGVAVILLTLLAFMSFPDPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVTHYFIYERNRRLLYLSVLALIIAAGIWLLRFLDDFL
                     MTTAFYVALFVLWVFLCFLYIQCENEMSMSE"
     gene            complement(548..912)
                     /locus_tag="ACHJ12_004810"
                     /pseudo
     CDS             complement(548..912)
                     /locus_tag="ACHJ12_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(926..1669)
                     /gene="traF"
                     /locus_tag="ACHJ12_004811"
     CDS             complement(926..1669)
                     /gene="traF"
                     /locus_tag="ACHJ12_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO4992473.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPVA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1662..1919)
                     /locus_tag="ACHJ12_004812"
     CDS             complement(1662..1919)
                     /locus_tag="ACHJ12_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO4992474.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYGCFSALADLQKPDERKSDERNEDE"
     gene            complement(1933..3837)
                     /gene="traN"
                     /locus_tag="ACHJ12_004813"
     CDS             complement(1933..3837)
                     /gene="traN"
                     /locus_tag="ACHJ12_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO4992475.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGDWKYMDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     GDGVLKGTECSEPGGTKTGVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGSCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(3931..4311)
                     /locus_tag="ACHJ12_004814"
     CDS             complement(3931..4311)
                     /locus_tag="ACHJ12_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992476.1"
                     /translation="MSLPAGYYRIEPDIRVLVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(4308..4946)
                     /gene="trbC"
                     /locus_tag="ACHJ12_004815"
     CDS             complement(4308..4946)
                     /gene="trbC"
                     /locus_tag="ACHJ12_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO4992477.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKPQ"
     gene            complement(5334..>5604)
                     /locus_tag="ACHJ12_004816"
     CDS             complement(5334..>5604)
                     /locus_tag="ACHJ12_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992478.1"
                     /translation="RLTGKALKLFPFALVMLFTVLFYFMPENVISDIISEWQRSDVSD
                     RIRVIRLYLGTTFLCTWAAVFLECLCSDEFLPGKRLNKNEEENNG"
BASE COUNT         1460 a         1522 c         1199 g         1423 t
ORIGIN      
        1 aaacctgact gattattatt ccgacatact catttcattt tcacattgaa tatacaaaaa
       61 acagagaaac acccataaaa caaacagagc aacataaaat gctgtcgtca ttaaaaaatc
      121 atccagaaat ctgagaagcc agatacctgc tgcaatgatt agggcaagca cagacaagta
      181 caataacctg cggttacgct cgtaaataaa ataatgcgtt accattacaa cagacagcgc
      241 cacatacatt accccggtca cactcagaat taacacattc atcagccagc tataatagcg
      301 attcatactg acatccggat ctggaaaact cataaaagct aataaggtca gtaaaataac
      361 cgcaacacca gacgcgatta accatctttc agaaatacta caattttctt tatccataca
      421 gaataaaacc atacttaaca aacaacatga actaaaagcc attcaccatc aggtgggggg
      481 acagaaaaga gttgtctctg tttgtctttt actccgtaaa tgagtttatt tacatattaa
      541 cagagcatta tttcattatg gtttctgatg gctggagtaa ccagtagaat ataatcattc
      601 ctgcaccaga aaacactgcg gcaacagcga gccacaaacg cttatgctca ccttctgata
      661 tgcctgaaaa atatcctgta tggagtagca gcagcaacca tgacaacacg cctgccagac
      721 ccggcagggc aggagggata tgccacagtt tatatttcat aataacaata ccttctgcca
      781 tcatcatgga cagggtgcaa acaacaaaac agtcccatga aaaaaacgcc tgtcagcagc
      841 aaggaaaatc actgtcagtg acatcactgt cacccgggcc agcaataaag gccataatga
      901 aataaaattc atttaaacga cctctttaaa aattcggttt aaaatcttca gaaacgttca
      961 ggaactgttt tgccaggtcg tcctgagaaa taaagccgta tgataacggg cggacactgc
     1021 cctgtttcgg gtcaaccagc atcatggccg ggaaatattt cacgccgagg cgctgcgcct
     1081 gcccctggtc agtccgggaa tccggcaata gcggattaat cacgccatcc acggaaacgg
     1141 gaataacact cagaccatac gtatcccgga agccattaat gacctgcgcc agctgcccgt
     1201 cgatggggtc ctgcccccgg taaaaaaaca tgatgccgta gtgttcagcc agtctggcaa
     1261 tggcctgccg ctgctgcgcc tggtctgccg ccagctggtt ccgtaccgtg ccgttgtaat
     1321 ggctgtactg caggttatag tccagttcag gatgtgccag catggccttt ctggcgctca
     1381 tggtgaaaag cccggcctgc tgagtccagt aattctgcag ccggaaatat ttcacaaaat
     1441 tatccacgcc gggatacaga atggcttcgt acagcgcccg ctttgttgcc gtctgcagcg
     1501 cggccagttt ctgcataata tccggctcct gacggggagc gactggtact ggtttatttt
     1561 ctttttcctt cggatttatt ttctcgttat accactgcca gcctgcatct ttccctgacg
     1621 ccggaaaaat aaagcagcag agtaacagcg gcagtaatgc tttattcatc ttcattcctt
     1681 tcatcacttt ttctttcatc aggtttctgc aggtcagcca gtgcagaaaa acaaccatac
     1741 agaataaaca ggagcacacc ggagtaaagg ataatgaaag cagcagcaag caccccgcct
     1801 gccgtggttt cttttaccgt atcccacgac cagataatta ccggggaaat gacaatcgct
     1861 gtaatcaaaa gccggataag aaaatcaaca agacgattcg acgtgaatat gactttcatt
     1921 attttcccga ccttactgcc ccacctgttt cagctgaccg gcaatctgct ctttcacttt
     1981 ttcggtcagc gcaccattat ccgggatttt ctggttattc atcaggtctt cgtagaagtt
     2041 agtgaagtcc agctggtcga acttaatacg ctgcagttca tccacggtta ttccccggca
     2101 gtcaggactt tttgcgctgc caaacccgat acgcaactgc ccgttacgcc cctgctgctg
     2161 gactatctgc gccagcttcg aatcaaactg gcagtaactg cgctttttct gcagacagac
     2221 acccagcact ttccgggagc agaactcgcc cacactcacc gtcagtttgt tgtcctttgc
     2281 ctttcccagc gccttttctt cgctgttgca cttcgccaga cccacatcct gaccccagcc
     2341 accatcctta cagcagttac tgaagccggc tgcggcctta cggcagaatt tcgcctcgcc
     2401 cgtgaaagcc ctgacatcca caccattcag ggcggcaaca tccttacctg cggcggcaag
     2461 cgcagccagc tgtgatactg cctcaccaaa atcactgttt tttccgctct gcgccttatc
     2521 gcattcaccg tcaaggcaga agacatcacc accacagacc atcacttttc ctgatgttct
     2581 ggactcgcag gagtacgtgg catactcatg cagacagctt ccctcatcgg aataaaacgc
     2641 gcactgacgt gaggccagcg tacaggccgg attatcgaca tatttctgac aggtgccgtt
     2701 atctgcggac tgtgtgacat atttatcccg gtatgcccag cacgcctgtg tgatgctcca
     2761 gggtttacct tccatcacac ctgttttcgt cccccccggc tctgaacact cagtaccttt
     2821 cagcacgcca tcccctttat taaacggaca actctccacc cactccaccc gtggaaccca
     2881 ttctttatca cggaccttca tcctgagttt cagcgtaaag gtggaagcac cactgacaag
     2941 cgattcatag accatcctgt caccattccc gtgcgggagg caattaccgt ttgcagtaca
     3001 gccactaccg gtcagaacct gcccctgtgt gacagaaaat ccggagggca caggtatggt
     3061 gaaagtcccg ctctgtcctg taaccagttg cacattaaag gctgtgttca taaagtcgta
     3121 acgggagtta aggaaatata gccctgcatg agccgacagc gaggcacttt ccactgtccc
     3181 ggcaacaggt acggtaacgc tgaacaccag tttcagaccg ttcatactga accggaactg
     3241 tgagtgcggt atcgtcacct cacgatattc atccatgtat ttccagtctc ctgtgatcct
     3301 ggctgtcctg gaacaatatt cctccaccat cgtgtcacgt tcacaggtat agtttgtgaa
     3361 ttcactgcgg tttatctgct gcgcactgca ctgctgtccg gtatttccca cgatactgtc
     3421 agcccggttc accacatccc tgccggtctg aataaacggc gcatccggtg acagaatgtc
     3481 tttcggcttg ttcatgaatg actctgtgat ggttttgccg gtttcactgg ttgcccattg
     3541 tgtggtgccg ttatttttca gaccgccatc cccgccggca gtcaccccac cgtaatactg
     3601 cgtctcatcc gggttcgcgt tatacccggg gatactctcc tgcggattaa aactctgaat
     3661 actgccaata ccctgcccct ggatctgacg ggcaaaatcg gaacctgccc ggtaatcact
     3721 gttgctgtcg gcaaacacag aaaagctcag gaaaaaaaca attgcacaaa tattgcgcgg
     3781 tcttccggag tcattaacat tcagagacct gaaaaaggta tttatctttt ctttcataac
     3841 ctgatccata ctggagaatg aagtgatgtc atctcaactt cagaacggtg ttgattttgg
     3901 tgtatggagc ttcctggcgt accgggatta ctactcagat aacgacttca gtaacaatat
     3961 cagcgtcctg aagtctgcac gcagtgaacg acgacagtac cggtgatacc agcaatgcgg
     4021 accttccggc tgaagccgga aacagagctg aaggtcactg ttaaaagccc ccttcaccga
     4081 ccatccccat gtcagacgtg gtattgctga ttcagcatcc tgccgcagtc gctgctcaag
     4141 aagggcagcc atttttaccg gacaggcaaa ggcaatataa ggcagcagct ttgtaacagg
     4201 gaaaccatgt ccctgacagg aggcatatgt acggaatccg tgaacgttca tcgcggccac
     4261 cagtacacgg atgtccggtt caatccggta atacccggca ggtaaactca ttgcggctta
     4321 tccccggtgc cgtccagcag tccctgagcc acctgccggc agtctcccgt ggcggccact
     4381 ttttccagcg cctgcctgac gcgcagattt ccccggatga tgtcgtgccc ctggctgcag
     4441 aacactacca gggccggcac gctgcggatg ccgtactgtg aaaacaacgt cgggtcgatc
     4501 tgaacaccat cggtcgcgcc atcttttacc agagacaaca cggcttcagc ggtagtcttc
     4561 aggtcattgt tcaccatgcc ccgcagtgtg gccggaatac cgtagtgccg ggtttctccc
     4621 agcatccgtt tcagtccctc ttcgggaatg gagaacgaca caaaatacac ggccccctgc
     4681 tgtggtttgt ctttctgagc agcctgctgt ttacggacca gttcatcgag aaactggtta
     4741 tcggcatgct gaaggggatt cgccctgacc tgctgttctg cccatgcctg cagttgtgtg
     4801 tccggctttt cacgcaactg ctgactcagt gtttcctgct gcttcaggaa ctggcggttt
     4861 tcgggcgtat tcacgttttc cgaagcatgg gcagaaagaa acaatcctgc tgtcagggcg
     4921 aataaaatgt atattttttt gttcatagaa ttctcctgca aacagacagt aattaatcgt
     4981 cataatgagg cgctcaatag agcgagtctg gagatagctg acgccatgtt aaacacaacg
     5041 aagatcgttg attaacttct ttctccattt atcttttttc tgagactggc agcaattaat
     5101 tgccagtctc tgtattgctc tgctgttatt acctttttct ccatactgaa agtgaccggg
     5161 atacagggag aaatacatcc tgatgcaagg ggtgctgcgg gccacattgt ttctgttctt
     5221 ttttcatctt ttatcctttt actgtttaat tatccaggat aaacgatacc ggatattcac
     5281 tctgttaaat cattaattca tttctgaccg atgcacataa aatgagtcag atattacccg
     5341 ttattctctt cttcattttt gtttaatctc tttcccggca gaaactcatc agagcagaga
     5401 cattccaaaa aaacagccgc ccaggtgcat aaaaaagttg ttcccagata tagcctgata
     5461 actctgatcc tgtctgacac atcacttctc tgccattcac tgataatgtc actgataaca
     5521 ttttcaggca taaaataaaa caacaccgtg aatagcatca ccagtgcaaa aggaaacaat
     5581 tttaatgctt tgcctgtcaa acga
//
LOCUS       DAZFFI010000201         5521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFFI010000201 DAZFFI010000000
VERSION     DAZFFI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.203"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            131..1234
                     /gene="proB"
                     /locus_tag="ACHJ12_004817"
     CDS             131..1234
                     /gene="proB"
                     /locus_tag="ACHJ12_004817"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4992479.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1246..2499
                     /gene="proA"
                     /locus_tag="ACHJ12_004818"
     CDS             1246..2499
                     /gene="proA"
                     /locus_tag="ACHJ12_004818"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4992480.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2614..2689
                     /locus_tag="ACHJ12_004819"
     tRNA            2614..2689
                     /locus_tag="ACHJ12_004819"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2647..2649,aa:Thr,seq:cgt)
     gene            complement(2816..3154)
                     /locus_tag="ACHJ12_004820"
                     /pseudo
     CDS             complement(2816..3154)
                     /locus_tag="ACHJ12_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3205..4161)
                     /gene="paoD"
                     /locus_tag="ACHJ12_004821"
     CDS             complement(3205..4161)
                     /gene="paoD"
                     /locus_tag="ACHJ12_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO4992481.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNWELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            complement(4171..>5521)
                     /gene="paoC"
                     /locus_tag="ACHJ12_004822"
     CDS             complement(4171..>5521)
                     /gene="paoC"
                     /locus_tag="ACHJ12_004822"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4992482.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
BASE COUNT         1225 a         1539 c         1557 g         1200 t
ORIGIN      
        1 ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgcttg cagatggttg
       61 gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg
      121 gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac
      181 aggcggatcg cgccgtctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca
      241 gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg
      301 tgagcacctg gggtacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcggc
      361 ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca
      421 cgtcgggcag atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc
      481 ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga
      541 tgctgtcgct acggcagaga ttaaggtcgg cgataacgat aacctttctg cgctggcggc
      601 gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc
      661 tgatccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc
      721 actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa
      781 attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag
      841 caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc
      901 ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga
      961 aatcacggta gatgaagggg caactgccgc cattcttgaa cgcggcagct ccctgttgcc
     1021 gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa
     1081 cctcgaaggt cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg
     1141 tattgccggg caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt
     1201 tgccgttcac cgtgatgaca tgattacccg ttaaggagca ggctgatgct ggaacaaatg
     1261 ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat
     1321 cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct
     1381 aacgcccagg atgttgctga cgcgcgtgcc aatggccttg gcgaagcgat gcttgaccgt
     1441 ctggcactga cgcccgcacg gctgaaaggc attgccgatg atgtgcgcca ggtgtgtaac
     1501 ctcgccgatc cggtggggca ggtaatcgat ggcagcgtac tggacagcgg cctgcgtctt
     1561 gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg
     1621 acggttgatg tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct gcgcggtggc
     1681 aaagaaacgt gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc
     1741 tgcggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt
     1801 gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgtggtgg cgctggtttg
     1861 cataaactgt gccgtgaaca gtcgacaatc ccggtgatca caggtggtat aggcgtatgc
     1921 catatttacg ttgatgaaag tgtagagatc gctgaagcat taaaagtgat cgtcaacgcg
     1981 aaaactcagc gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc
     2041 gccgatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacattacac
     2101 gcagatgcag ctgcactggc gcagttgcag gcaggccctg cgaaggtggt tgctgttaaa
     2161 gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat
     2221 cttgacgatg ccatcgccca tattcgtgaa cacggcacac aacactccga tgcgatcctg
     2281 acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac
     2341 gttaacgcct ctacgcgttt taccgacggc ggccagtttg gtctgggtgc ggaagtggcg
     2401 gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac
     2461 aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa
     2521 gtagccattt gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac
     2581 cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag
     2641 cgcattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat taaaatcaaa
     2701 gagttacccc atatttaaat acaccacgtt tcctcctgtg ccgcatttgt gccattgtaa
     2761 ccttggcaat tcatcaaaat actgttctga catcaggcag tgcaggtgca gctatttaaa
     2821 ccaattgctg ccgccattct ttgacgtagt caatcagggc gcggagcttt ggtgcaatat
     2881 tgcgacgctg tgggaaatac agatagaagc ccggaaattg tggaagaaag tcatcaagca
     2941 gcgatacaag cttaccgctt tcaatatatg gcctgaaagt ttcctgagtg gcaattgtta
     3001 ttcctccgcc ggcaagagcc agcctcaaca tcagacgcag atcattagtc gtaatctgcg
     3061 gttcaatcgc aaggtcgaaa gttctcccgt tttcttcaaa tggccagcga taaggcgcaa
     3121 cctccgggga ctgacgccag ccgatacact tatgggtatt tcccccggag gcgagaaagc
     3181 gctctccacg cccggccgca aggatcagga cgacgggggc aggcatgaat cctcctcatg
     3241 atggagacgt acagaggcga tttctgccag cacggagagt gccagagtat gcgcatcccg
     3301 ggctttgggg aatatcccga cgggggcccg gatttgcgct gtctcctccc tggaccatcc
     3361 cagctcgtgg agcttttgca gacgtaacgt gtgggttcga tggctgccca atgcgccgag
     3421 ataaaagggt tttgcttctc gcgcggcctg caatgctggc agctcccagt tgagatcatg
     3481 gcacagcaaa atgaccgccg tgtcggtatc gatctgagcg ctggctgagg ccggaaaaag
     3541 atcgaagata tggctgtcat agcctgtggc tgctgcaaga ctcgcggttg cctgcgcctc
     3601 aagagaacgt ccgtaaatca tcagcctgac gcatggcctg aaccccacct caaagccatt
     3661 gagattccag cccgttcggg tttgcgtggg caggcacacc aacgattgtg cttgcggatc
     3721 gtagcgcagc cccgccggtt ttctctgttc cagacggttc agcacggcga gcagaggctg
     3781 tgccgagcgt agtttatgga gcgtcagcgt gatcccaccg ccgcagggca gaacgatgtc
     3841 aaaccacggc gaaccttcgc catagcgaat ttcgcgatcg cggcctgagc ccatcatctc
     3901 cagcgcttca aaagcggcag cggcctccac gcagccgcca gagacaaaac cgcagtaacg
     3961 accatcttcg cgaaccacca tctgcgcccc gagcgggcgc gccgcgccgc cgtgtatctc
     4021 caccagcgtc accagcaccg ctcctttacc ggacattagc gcctctacgg cgaagcgcag
     4081 aatggtccgg tggtcatcgg tgagaaacgc ctgctctggc ttatgccagt gttcgtcttt
     4141 atcaaaaagc gggtatgaca ttgtttctcc ttaaaccaca tccggcagct tatcgagcag
     4201 cttatccaga gtgatgggat aatcccgtac ccgaataccg gtggcgttat acaccgcgtt
     4261 ggcgatagcc gcgctcacgc cgcacaggcc caactcaccg acacctttgg ccttcatcgg
     4321 ggaggatatg gggtcggtat catccaggaa aatcacctcc tgttttggga tatccgcatg
     4381 gaccggcacc tcataccccg ccatatcgtg attaacgaag tagcccaaac ggtcatccac
     4441 cgccagctcc tccatcagtg ccgcacccat gcccatagtc attgcgccaa tgacctggct
     4501 gcgcgcagtt ttcggattca ggatgcgtcc tgcagcacac acagcgagca tacgccggac
     4561 ccgaacttct cccgtcgcgc tatgcacgcc gacctccaca aaatgcccgg caaaggtcga
     4621 ctgctggtac tctttgctca gtgttccgaa ttcaatgctc tcttccgctg tcagtctgcc
     4681 gcctgcggtg gcttcatgta gcgtggcgct tcgggtaccg ttggtaatct tgccgtcggc
     4741 aaactgcgac tgctcaggat caaacccgac tgccgaggca atcatttcgc gaagcttcat
     4801 acaggcggcg taaacgccgg aggtggaggt attcgcgccc cattgtccac cagaacccgc
     4861 agaaaccggg aaactggaat cgccgaggtg aaccgcaacc tgctccagcg gtacgccaag
     4921 catttccgct gccgtctggg ccagaatggt gtagctgccg gtgccaatgt cggtcatgtc
     4981 cgtttctacg gtaacggtgc cgttttgttc gaggtgaacc cgagcacccg atttttccag
     5041 cagattattg cgaaagcccg ccgcaacacc gtggccgact agccactccc cgtcgcgcac
     5101 ctgtccgggt gtggcgttgc gctgcttcca gccaaattta tccgctccgg tgcgcaagca
     5161 ctcgataagc tgacggcgag agaagcagcg cgtcgggtcg gcggggtcaa cctgagtgtc
     5221 attcaggatg cgaaactcga cgggatcgat gcccgctttt tccgccagtt cgtcgatcgc
     5281 gatttcgagc gccatcagac cgggggcttc gccgggcgca cgcatggcgt tcccttccgg
     5341 caaatcaagc gtggcgagcc gcaggccggt atgacgattc gccccggcgt agagtaattc
     5401 gctttgctgt accgccgttt ccggcgtgcc gccgggcagg tttccagacc agctttcatg
     5461 tgagatagcg gtgattttcc cgctctggtc ggcaccgata cgcaagtgct gaagggtggc
     5521 g
//
LOCUS       DAZFFI010000202         5407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFFI010000202 DAZFFI010000000
VERSION     DAZFFI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.204"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            109..1443
                     /gene="garP"
                     /locus_tag="ACHJ12_004823"
     CDS             109..1443
                     /gene="garP"
                     /locus_tag="ACHJ12_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4992483.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1459..2229
                     /gene="garL"
                     /locus_tag="ACHJ12_004824"
     CDS             1459..2229
                     /gene="garL"
                     /locus_tag="ACHJ12_004824"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4992484.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIATTEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2259..3149
                     /gene="garR"
                     /locus_tag="ACHJ12_004825"
     CDS             2259..3149
                     /gene="garR"
                     /locus_tag="ACHJ12_004825"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4992485.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3246..4391
                     /gene="garK"
                     /locus_tag="ACHJ12_004826"
     CDS             3246..4391
                     /gene="garK"
                     /locus_tag="ACHJ12_004826"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4992486.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4424..4800
                     /gene="rnpB"
                     /locus_tag="ACHJ12_004827"
     ncRNA           4424..4800
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ12_004827"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5196..>5407)
                     /locus_tag="ACHJ12_004828"
     CDS             complement(5196..>5407)
                     /locus_tag="ACHJ12_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992487.1"
                     /translation="PQINYTMDLNIEDSSSEELLSDNTLEKDENSPDNGFEVGEYNTY
                     EAYNSENQYFTREDYTYNYDLLNAI"
BASE COUNT         1287 a         1276 c         1513 g         1331 t
ORIGIN      
        1 tgcttatact aattcccgag ttcattgccg ttacggcata aatgaaactc ataaataaca
       61 ccttccccat aataataaaa atcagcataa gtacccgagg taaataaaat gattctggac
      121 accgttgacg aaaaaaagaa aggcgtgcat acccgctatt taatattact gattattttt
      181 attgttaccg ccgttaacta cgccgatcgt gcaacgctgt ctattgctgg taccgaagtg
      241 gcaaaagagt tgcagttaag tgcggtttcg atgggttaca tcttttctgc ttttggctgg
      301 gcctacttgc tgatgcaaat ccccggcggc tggctgcttg ataagtttgg ctcgaaaaaa
      361 gtttatacct atagcctctt tttctggtcg ctattcactt tcctgcaagg ctttgttgat
      421 atgttcccgc tggcctgggc agggatctcc atgttcttta tgcgctttat gctcggcttc
      481 tcggaagcgc catcattccc ggcgaacgcc cgtattgtcg ccgcctggtt cccgacgaaa
      541 gaacgtggta ctgcctccgc catctttaac tcggcgcaat atttctcgct ggcgctcttt
      601 tcgcctctgc ttggctggct gactttcgcc tggggctggg agcacgtctt taccgttatg
      661 ggggtgattg gttttgtgct gacggcgctg tggatcaaat tgattcataa cccgacagat
      721 cacccacgta tgtctgcgga agagctgaag tttatctctg aaaatggcgc ggtggtcgat
      781 atggaccaca aaaagccggg cagcgcggca gcaagcggtc ccaaactgca ttacatcaag
      841 caattgctct ctaaccgcat gatgttgggc gtatttttcg gacaatattt tatcaacacc
      901 atcacctggt tcttcctcac ctggttcccg atttatctgg tgcaggaaaa aggcatgtcg
      961 attctgaaag tgggtctggt cgcctcgatt ccggcactgt gtggttttgc gggcggcgtg
     1021 ctgggaggtg tcttctcgga ttatctgatc aaacgcggtt tatccctgac cctggcacgt
     1081 aagctaccga ttgtgctggg aatgttgctg gcttccacca tcatcttatg taactacacc
     1141 aacaacacca cgctggtggt catgctgatg gcgctggctt tttttggcaa aggatttggt
     1201 gctctgggct ggccggtgat ttctgacacc gcgccgaaag agattgttgg cctctgtggc
     1261 ggcgtcttta acgtctttgg caatgttgcc tccattgtca ctccactggt gattggctac
     1321 ctggtaagtg aactgcactc cttcaatgca gcactggttt tcgtgggatg ttcagcgctg
     1381 atggcgatgg tctgctacct cttcgtagtt ggcgacatta aacgtatgga attgcagaaa
     1441 taagcaaagg tataagcgat gaataacgat gttttcccga ataaattcaa agccgcactg
     1501 gctgcgaaac aggtacaaat tggttgctgg tcagcactct ctaacccgat tagcactgaa
     1561 gttcttggtt tggctgggtt tgactggctg gtgctggatg gcgaacatgc gccaaacgat
     1621 atctccacgt ttattccgca gttaatggcc ctgaaaggca gcgccagcgc gccagtagtg
     1681 cgagtgccga ccaacgagcc ggtaattatt aagcgtcttc tggatatcgg tttctataac
     1741 ttcctgattc cttttgtaga aacaaaagag gaagcagagc aggcggtggc atcaacccgt
     1801 tacccaccgg aaggcattcg cggcgtctcc gtttctcacc gcgccaatat gtttggcacc
     1861 gtggcggatt atttcgctca gtcgaacaag aacatcacca ttctggtcca gatagaaagt
     1921 cagcagggcg tagataacgt cgatgccatt gccactaccg aaggtgtaga cggtatcttc
     1981 gtcggcccca gcgatctggc tgcggcatta ggccatctcg gcaatgcctc gcacccggat
     2041 gtacaaaaag caattcagca catttttaac cgtgccagtg cgcacggcaa acccagcggt
     2101 attctcgcgc cggtcgaagc cgatgcgcgt cgttatctgg aatggggcgc gacgtttgtg
     2161 gctgtcggca gcgatcttgg cgtcttccgc tctgccactc agaaactggc tgataccttt
     2221 aaaaaataac caccaccgca acaagagaga tgattgatat gactatgaaa gttggtttta
     2281 ttggcctggg gattatgggt aaaccaatga gtaaaaacct tctgaaagca ggttactcgc
     2341 tggtggttgc tgaccgtaac ccagaagcta ttgctgacgt gattgctgca ggtgcagaaa
     2401 cagcgtctac ggctaaagcg atcgctgaac agtgcgacgt catcataacc atgctgccaa
     2461 actcccctca tgtgaaagag gtggcgctgg gtgagaatgg catcattgaa ggcgcgaagc
     2521 caggtacggt attgatcgat atgagttcta tcgcaccgct ggcaagccgt gaaatcagcg
     2581 aagcgctgaa agcgaaaggc attgatatgc tggatgctcc ggtgagcggc ggtgaaccga
     2641 aagccatcga cggtacgctg tcagtgatgg tgggcggcga caaggctatt ttcgacaaat
     2701 actatgattt gatgaaagcg atggcgggtt ccgtggtgca taccggggaa atcggtgcag
     2761 gtaacgtcac caaactggca aatcaggtca ttgtggcgct gaatattgcc gcgatgtcag
     2821 aagcgctaac gctggcaacc aaagcgggcg ttaacccgga cctggtgtat caggcaattc
     2881 gcggtggact ggcgggcagt accgtgctgg atgccaaagc gccgatggtg atggaccgca
     2941 acttcaagcc gggcttccgt attgatctgc atattaagga tctggcaaat gcgctggata
     3001 cttcccacgg cgtcggcgcg caactgccgc tcacagctgc ggttatggag atgatgcagg
     3061 cactgcgagc agatggttta ggaacggcgg atcatagcgc cctggcgtgc tactacgaaa
     3121 aactggcgaa agtcgaagtt actcgttaat gacgtcgtgc ccggatggcg tattgcaatc
     3181 cgggcctgga atccaggccg aataagagaa acgccctccg gcgtcatgtg gtaacaggca
     3241 taggtatgaa aatcgtaatc gccccagact cttataaaga aagtttatct gccagcgagg
     3301 ttgcgcaggc gatagaaaaa ggatttcggg aaatttttcc tgatgcacag tacgtttcta
     3361 ttccggttgc cgacggtggc gaaggaacgg tggaagcgat gattgcagcc acccagggtt
     3421 ctgaacgtca cgcctgggtt acagggccgc tgggcgagaa agtgaatgcc agttggggga
     3481 tctccggcga tggcaaaacc gcgtttattg aaatggcggc ggccagtggg ctggagctgg
     3541 tacctgcgga aaaacgcgat ccactcgtga ccacttcacg cggcacaggc gagttaatcc
     3601 tgcaggcgct ggagagcggc gcgacaaaca ttattatcgg cattggcggt agcgctacaa
     3661 atgatggcgg cgcaggcatg gtacaggcgc tgggggcgaa attatgcgac gccaacggca
     3721 atgaaattgg ttttggcggc ggtagtctta atactctgaa tgatattgat atttccggcc
     3781 tcgatccgcg cttaaaagat tgcgtcattc gcgtcgcttg tgatgtcacc aatccgctgg
     3841 tgggcgataa cggcgcatcg cgcatctttg gcccacaaaa gggagccagt gaagcgatga
     3901 ttgttgagct ggacaataac ctctctcact atgccgatgt cattaaaaaa gcgctgcatg
     3961 ttgatgtgaa agatgtcccc ggcgcaggag ctgcgggcgg tatgggtgcg gcgctgatgg
     4021 cgtttcttgg tgcggaactg aaaagtggta ttgaaatcgt cactacggcg ctgaatctgg
     4081 aggaacatat tcacgattgt acgctggtga tcaccggtga agggcgtatt gacagccaga
     4141 gtattcacgg caaggtaccg attggcgttg caaacgtggc gaagaagtac cataaaccgg
     4201 tgattggcat tgcgggtagc ctgaccgatg atgttggcgt tgtacatcag catggcatcg
     4261 atgcggtctt cagcgtattg accagcatag gtacgttgga cgaagcattc cgcggggctt
     4321 atgacaatat ctgccgtgct tcacgtaata tcgccgcgac actggcgatt ggaatgcgca
     4381 acgcggggtg acaagggcgc gcaaaccctc tatactgcgc gccgaagctg accagacagt
     4441 cgccgcttcg tcgtcgtcct cttcggggga gacgggcgga ggggaggaaa gtccgggctc
     4501 catagggcag ggtgccaggt aacgcctggg ggggaaaccc acgaccagtg caacagagag
     4561 caaaccgccg atggcccgcg caagcgggat caggtaaggg tgaaagggtg cggtaagagc
     4621 gcaccgcgcg gctggtaaca gtccgtggca cggtaaactc cacccggagc aaggccaaat
     4681 aggggttcat aaggtacggc ccgtactgaa cccgggtagg ctgcttgagc cagtgagcga
     4741 ttgctggcct agatgaatga ctgtccacga cagaacccgg cttatcggtc agtttcacct
     4801 gatttaacga aaacccgcca gcgtaaaccg gcgggttttt gcttttggag gggcagaaag
     4861 atgaatgact gtccacgacg ctatacccaa aagaaagcgg cttatcggtc agtttcacct
     4921 gatgtacgta ataaaccgtt ccggcgggtt tcagattgtt gagtgcgctt tattcatgcc
     4981 ggatgcggcg tgaacgcctt atcctgccta caaaagcacg caaattcaat atattgcaga
     5041 gatggcgtag atactgataa gcgaagcgca tcaggcattt ttgcggttgc catcagtttc
     5101 aaacccgcgt cagtgagttt ttgcttttgg tgcacagggt aagatgaata aatatctacg
     5161 gtattaataa tagactttgt aagaagttat taagtctata ttgcatttaa aaggtcgtaa
     5221 ttatacgtat agtcctctct ggtaaaatat tgattctctg agttatatgc ttcatatgtg
     5281 ttatattccc cgacctcaaa accattgtcc ggagaatttt cgtctttctc gagggtgtta
     5341 tctgaaagta actcttctga gctagagtct tctatgttta aatccatagt ataattaatt
     5401 tggggaa
//
LOCUS       DAZFFI010000203         5296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFFI010000203 DAZFFI010000000
VERSION     DAZFFI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.205"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            127..1485
                     /gene="mltD"
                     /locus_tag="ACHJ12_004829"
     CDS             127..1485
                     /gene="mltD"
                     /locus_tag="ACHJ12_004829"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4992488.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NMPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1533..2303)
                     /locus_tag="ACHJ12_004830"
     CDS             complement(1533..2303)
                     /locus_tag="ACHJ12_004830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4992489.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DTSVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2381..3181)
                     /locus_tag="ACHJ12_004831"
     CDS             complement(2381..3181)
                     /locus_tag="ACHJ12_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4992490.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3422..4336
                     /gene="yafC"
                     /locus_tag="ACHJ12_004832"
     CDS             3422..4336
                     /gene="yafC"
                     /locus_tag="ACHJ12_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4992491.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPRGAVREA"
     gene            complement(4333..5136)
                     /gene="dkgB"
                     /locus_tag="ACHJ12_004833"
     CDS             complement(4333..5136)
                     /gene="dkgB"
                     /locus_tag="ACHJ12_004833"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4992492.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLKAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1393 a         1265 c         1314 g         1324 t
ORIGIN      
        1 gcaacaacct gaagaacgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
       61 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
      121 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
      181 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
      241 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
      301 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
      361 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
      421 gagccgtata tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg
      481 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
      541 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
      601 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag
      661 cgtctgaaca aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
      721 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
      781 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
      841 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
      901 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
      961 gggatttccg tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc
     1021 gcaagtggtc cgcaatacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     1081 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tatgccgctt
     1141 aacagccgag tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     1201 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     1261 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat
     1321 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     1381 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat
     1441 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     1501 aaaggcaccg attcccccgg tgcctttttt atttatgctg ctttatgtgc ttctaccatg
     1561 atgatatcac tggtgaaaga gccatcattc tgcaaggcaa aatacgttct cacctctgtc
     1621 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
     1681 gcgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
     1741 ttggcttcat tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta
     1801 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctggatgacc cggagacatt
     1861 acgtccatca caatcatcct accgccaggt ttcaatatcc tattcacttc acgcagtgct
     1921 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
     1981 tcggcaaatg gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg
     2041 gcttcggcag cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc
     2101 gcgctcacgt tctgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
     2161 acacttgtat caggataatc agccagacgc accgccaggc gctgtaaatc tcgcccggat
     2221 gcatgtacgg tactggttaa atactcactg gcctgcgagc taaactgctt ttctacgtgg
     2281 tcatggtggg attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaagggcag
     2341 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     2401 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt acagaagctt
     2461 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg acggccaaac gcccgacggc
     2521 gctgatcgtc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     2581 cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt
     2641 gatgagctat ctgatcgcca ataggaagta actgcttact ataaacatcc acgcccagac
     2701 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtatcaggt aatggataga
     2761 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     2821 aatacactgg atgtgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagtacga
     2881 aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     2941 ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg
     3001 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     3061 cagtacttaa cggttctccg ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     3121 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     3181 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     3241 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     3301 tacactggtc agaattttgc tcattattga gaacattaaa tcggatgaca tatcacgcat
     3361 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     3421 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtagaaa gcggcagctt
     3481 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cagtgaaaaa
     3541 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     3601 ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcagcggc
     3661 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc
     3721 aactccagtg gtgctgcact ttctgatgcc gttgattaag ccttttcgtg aacgctatcc
     3781 ggaagtcact ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     3841 tgatgtcgcg atacgcgccg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     3901 taatagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     3961 aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa
     4021 tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg gtttgtcatc
     4081 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     4141 cgactacatg atcgacaaag aaatcgttcg cggagaattg gtggagttaa tggcagataa
     4201 agtgttgcca gtggaaatgc cattcagtgc agtctattac agcgaccgtg cggtaagtac
     4261 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccagaggagc
     4321 tgttagagag gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt
     4381 gcaatccagg gcggcgatcg cttttttatc tttggcatcg agctgtaaat tttgtgcctt
     4441 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     4501 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     4561 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatggat
     4621 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     4681 ctggttagta gcgatgtttt cagcgccaac agcagcaatc gccttttcca tcaatgggat
     4741 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt tcagcagcgc
     4801 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     4861 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     4921 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     4981 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     5041 tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag
     5101 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     5161 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     5221 ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac
     5281 tcgaggcttc ttaata
//
LOCUS       DAZFFI010000204         5250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFFI010000204 DAZFFI010000000
VERSION     DAZFFI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.206"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..227
                     /locus_tag="ACHJ12_004834"
     CDS             <1..227
                     /locus_tag="ACHJ12_004834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992493.1"
                     /translation="KKCTVVNMSDINFGIFFIIAFGFITVGSFFIVLIRFLERAETIL
                     IGHLDLKSKAIIHGLIILFIYWTLKFFAES"
     gene            339..884
                     /gene="ddp1"
                     /locus_tag="ACHJ12_004835"
     CDS             339..884
                     /gene="ddp1"
                     /locus_tag="ACHJ12_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="HFO4992494.1"
                     /translation="MKQIKFRLDDAKHHDLLICLKSLYPDEPALTVSKGMKLLANALL
                     KSKKTNQIQNDYADENNFIKTTLYLTGKQRADIEKAANRHGWTLSRECRYRIQTTLNN
                     RLDFFDQELLMMNRCRNTIDKIGRNFHYIIVNDQTRVLDKDGFYQDAERLKTEIFSLK
                     NQFENYIMLCKGRTVSNKVDV"
     gene            1992..2723
                     /locus_tag="ACHJ12_004836"
     CDS             1992..2723
                     /locus_tag="ACHJ12_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4992495.1"
                     /translation="MTKKNVDQPMTMTVSLRGKNVVMSNAISRAAQSLSLAEKRIIFA
                     AIAQMGGKYAPVRITAQEYANTFGMPLKQAYSQLKEASENFFNRYIRFKLNEVSEIDV
                     DVWKIRWLGAYKYNDGEANVLLHFTPQVMPYLCELEDNFTKYQLSQACALRSVHSWRL
                     LELFEQMNTNKENNGWLSISLEDFHHAMESPDSYKTTFGLLRKYMIEPAVKELTEKDH
                     WMIDWKPIKKGRKVVRLEFWFRKEN"
     gene            3145..3903
                     /locus_tag="ACHJ12_004837"
     CDS             3145..3903
                     /locus_tag="ACHJ12_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO4992496.1"
                     /translation="MKKRIISAVLASLSVISVAQAGIPVAIDANPEWAVEAGRWTERL
                     KQWAETVKHYENQINAYKQELLSKTGIRDVQGLVQSAQSVSQELMQIYDQGNAFIDDY
                     IKNPESALSEQAKSLLSDYRITDTCQNLGYSGDQVRGCEATFLSQLASVEYGNKLESK
                     LRQDNQTMKDLIEQVKNAKDTKATQDATNAVALEQLKFEKLKFQYQMYIDKQKQLSEY
                     KKEMAKASFRHEQINAKPIDYREAFSTQDFDLIK"
     gene            3913..4182
                     /locus_tag="ACHJ12_004838"
     CDS             3913..4182
                     /locus_tag="ACHJ12_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO4992497.1"
                     /translation="MKYIVLVFLASLSFILTGCNEDTKSVDWWTNHPKEAVDKYKECK
                     KTGSNSDNCKNVRRAGLIIADTYPPMSEIYKQEARELDKKLGVSK"
     gene            4196..>5250
                     /locus_tag="ACHJ12_004839"
     CDS             4196..>5250
                     /locus_tag="ACHJ12_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO4992498.1"
                     /translation="MATDNTYPVYDGNFFRATHDIILRAVEKSTSGMFDNYINIAYTL
                     GKYGVSLYILWYAFTVLARKQQTPVPDFIWNLCRFSIILMFVKNTGGWLDNANQAIDG
                     LKETLAGGDPWLWMDQLWDKVITVGKKVWHLDTDTIPVVGVAAALLTYAGGILAMIVC
                     AIVFFSAEITLKILSITAPLFIMCLMFGFLRQMFNNWLQLIFSSLLIFMFAALALRAG
                     MWQLNGTLSSTLSSATAPNLFEAGATVLVSGIFMAWIIWQAKTYASQLAGVGVEGAMQ
                     GAAAMGIGAGIFGASRMTRGALGMGRNAGIGAWKGLRRQEGGFGQSPGITGKAANLAG
                     QGVNIGAKKLRQAAIERA"
BASE COUNT         1579 a          914 c         1177 g         1580 t
ORIGIN      
        1 aaaaaaaatg tacggtggtt aatatgagtg atattaactt tggtatcttt tttataatcg
       61 catttggctt cattactgtt ggttcatttt ttattgtgct tatccgtttt cttgaacgtg
      121 cagaaaccat attgattggt catttagatt taaaatctaa agccattatt catgggttaa
      181 ttatattatt tatatactgg acgcttaaat tttttgcaga atcttaatca aagaaccgac
      241 tgtaggtcac cgggcaaacg gccaaaatgg aaccattttg cggcgtttgc cctatcctgc
      301 atcggagtaa atattcatgt gctgtaggaa gtgcaaatat gaaacaaatc aaattcaggc
      361 ttgatgatgc caagcatcat gacctgctga tctgcctaaa gtctctttat cctgatgaac
      421 cagccttgac tgtatcaaaa ggtatgaaac tgcttgccaa tgcattgctg aaaagcaaaa
      481 aaactaacca gatacaaaat gattatgctg atgaaaacaa tttcatcaaa accacgcttt
      541 acttaacagg taaacaaagg gctgatattg aaaaagcagc taaccgtcat gggtggacat
      601 tatcacggga atgtcgatat aggattcaaa ccacactgaa taataggctt gatttcttcg
      661 accaggaact gctgatgatg aatcgctgcc gaaatactat agataaaatt ggtcgtaatt
      721 ttcattatat cattgttaat gatcagacca gggttcttga taaagatggt ttctatcagg
      781 atgcggaacg tcttaagaca gaaattttta gtcttaaaaa tcagtttgag aattacatta
      841 tgttatgtaa ggggagaaca gtttcaaaca aggtagatgt atgaagtcca cattgataac
      901 taaatctagt ttgtattgtt gaataaacta catgttgctg atagagtctt tgcgagatgt
      961 gaatttatcc ttttgttttt ttctttgctt cggggtcatc cgagtcgaag gagcgtctag
     1021 tagaaacgga actactggca aatttgttac tatttcagtc ttgatatagc tgtaaactgt
     1081 ttatagtata aactttgcgc aataataaaa aatctatctc catagagatg tgatttatta
     1141 cgagcaacta cggttagctt gtgttttgtc acgtctaaac gttagtcagt tccagtaatg
     1201 agatcatcgt tgttattcct gtgcaaacgg cctttgcagg gagctggcgc ggtctcctgc
     1261 ttcctgaaag cccctggtta ggggtatcgg cgcgcctgac ctcctgaaac gctctgtaag
     1321 cgtttttcgc gccttccatg ccctgacggg catttaaaac ctgtttcgtg cgtctgagag
     1381 cttctgagcg cgttttaagg gccaatatga agaaaaccat taccaccacc agaaaatcgc
     1441 gttctggtgc tctggatggc tcacagaacg cattttcgaa cagaacagca acggaatcgc
     1501 cgacaacatg cgagaggtgg taccgctcgc cgcagtcgaa cggcaggagc gtagcgactg
     1561 agtgagcgag gaagcggaag tgagtctgca ggtgagttga gcacgtacgc atagcggtta
     1621 aaaaacgagc ggattgtttt taggtattgt tgctctagct taacgtgatc tgtcctcttg
     1681 gtttgagcat ggttatccac agattttggg ggcaaattcc tttttaaacc ctttttatct
     1741 tttcttttta aaaccttttt agacgccaaa actcttttaa aattatgagg ttacagagtt
     1801 aaaagtaagt tttttcgggt taaaagtaag ttttttcggg ttaaaagtaa gttttttcgg
     1861 gttaaaagta agttttttcg ggcattatcc acagctaact gtaagagcaa aaaatgattt
     1921 tacttttcat tttttgtaag tatgatttac gtacttactc ttgccctgta catcttactt
     1981 ttgagtgaat aatgaccaag aagaatgttg accaaccaat gacgatgact gtcagtttac
     2041 gtggcaaaaa tgttgtcatg agtaatgcta tatctcgtgc cgcgcaaagc ctttctttgg
     2101 ctgaaaagcg catcattttt gcagctattg ctcagatggg tgggaaatat gcgccagtaa
     2161 ggattacagc acaggaatat gctaacacct ttggaatgcc tttaaagcaa gcatattcac
     2221 aattaaaaga ggcttcggaa aatttcttta atagatatat tcgctttaaa ttaaatgaag
     2281 ttagtgagat tgatgttgat gtatggaaga ttagatggtt aggggcgtat aaatataatg
     2341 atggtgaagc taatgttctt ttacatttta cgcctcaagt aatgccatat ttatgcgaac
     2401 tggaagataa ttttacgaag tatcagctat ctcaagcttg cgcattgcgt tcagtacatt
     2461 catggcgttt gttagaactc tttgaacaaa tgaatacgaa caaggaaaat aatggatggt
     2521 tatccatatc gttagaagat tttcatcatg ctatggaatc acccgatagt tataaaacaa
     2581 cgtttggatt acttcgtaag tatatgattg aaccggcggt aaaggagtta acagagaaag
     2641 atcactggat gatcgactgg aaacctatta agaaggggag aaaagtcgtt cgtcttgagt
     2701 tctggtttag gaaagaaaac taatcatatc aatgattaac gctggtaatg agggctgcca
     2761 ccctcactac cagctaacca caacaacctg ttcggaggct gtatggctgg cccaagtata
     2821 tctgaatatc agttaaaaac ccgcaaagaa ttttacgctt atcttgatag taagaggaaa
     2881 gaaggaaact cgagatttag tttatccctt gagaaagacc ctttctcttt ttctttatat
     2941 cttttgttca caagtaatga aggtattcgt aatagtgatt taatccgtcg cttccgaatt
     3001 actagcaggg gcacgaggtt tattgtaagt aactttaaat tctggcttaa ggaatacaat
     3061 cgcggtatgt ttaagtgatg ttagccctct ctttaattaa aggagagggc taattatcag
     3121 gattatattt agtggagatc aataatgaag aaacgcatta tatcggcggt gctcgcttcg
     3181 ctctcggtta tttccgtcgc tcaggcgggg atacctgttg ccattgatgc caatccggaa
     3241 tgggcggttg aagccggacg atggacagaa cgtctgaagc aatgggcaga aacggtgaaa
     3301 cattacgaaa atcagataaa tgcgtacaaa caggagctgc tgtcaaaaac gggtatccgt
     3361 gatgtgcagg gactggtgca gtccgcacag tcagtgagtc aggaactgat gcagatttat
     3421 gatcagggga atgcttttat tgacgattac attaaaaatc ctgaaagtgc gttatcggaa
     3481 caggccaaat cgttattgtc agattacagg ataacggata cctgccagaa cctgggatat
     3541 tctggtgacc aggtacgggg atgtgaagcg acgttccttt ctcaactggc aagcgtggaa
     3601 tacggtaaca agctggagag caagcttcgt caggacaatc agacaatgaa agaccttatc
     3661 gagcaggtta aaaatgcgaa ggatacgaaa gccacgcagg atgcaacaaa cgccgttgca
     3721 cttgaacaac tgaagtttga gaagctcaaa tttcagtatc aaatgtacat cgataaacaa
     3781 aaacaactgt ctgaatataa aaaggaaatg gcgaaagctt cttttcgtca tgaacaaatt
     3841 aatgcaaaac caattgatta cagagaagca ttttcaacac aagactttga tttgatcaaa
     3901 taaggggttt ttatgaaata tatcgtcttg gtatttttag catctttatc atttattctt
     3961 accggatgta atgaagatac aaaatctgtc gattggtgga ctaatcaccc gaaagaagct
     4021 gtcgataaat ataaagaatg caagaaaaca ggaagtaact cagataattg taaaaacgta
     4081 agacgtgcgg gacttataat cgctgacact tatcctccga tgagtgaaat atacaagcaa
     4141 gaagccagag aacttgataa gaaattaggc gtttcaaaat aactgagggg gttatatggc
     4201 tacagataat acttatcccg tatatgatgg aaatttcttt agagcaactc atgatataat
     4261 attacgagca gttgagaaat caacatcagg aatgtttgat aattatatca atattgctta
     4321 tacactgggt aaatatggtg tttctttata tatcttatgg tatgcattca ctgtcttagc
     4381 aagaaaacaa caaactcctg ttccagattt tatctggaat ttgtgcaggt tttcgataat
     4441 tttaatgttc gttaaaaata cagggggatg gcttgataac gccaatcaag ctatagatgg
     4501 cttaaaagag actctcgccg gtggtgatcc ttggttatgg atggatcaac tttgggataa
     4561 agtaattact gttggtaaaa aggtttggca tcttgataca gatacaatcc ccgttgtagg
     4621 tgttgctgcg gccttgctta cttatgccgg aggcatttta gcaatgatcg tgtgtgcaat
     4681 agtatttttt tcagcagaaa ttacgttaaa aatcctttct attacagcac cgttatttat
     4741 tatgtgtctg atgttcggtt ttcttcgtca gatgtttaat aactggttac agcttatttt
     4801 cagttcatta cttattttta tgtttgcagc actggcgtta cgtgcaggta tgtggcaatt
     4861 gaatggaaca ttaagcagca ctttatcttc agcaacagct cctaatttat ttgaagcagg
     4921 tgcgacagtt cttgtctcag gtatctttat ggcatggatt atctggcagg caaaaaccta
     4981 tgcttcacag cttgctggcg tgggtgttga aggtgccatg cagggcgcag ccgctatggg
     5041 gattggtgct ggcattttcg gtgcatcccg tatgacgcgt ggtgcacttg gtatgggcag
     5101 aaatgccggt attggtgcat ggaaaggtct gcgtcgtcag gaaggcgggt tcggacagtc
     5161 tccgggaata acgggcaaag ccgctaacct ggccggacag ggcgttaata ttggtgccaa
     5221 aaaacttcgt caggccgcta ttgagagagc
//
LOCUS       DAZFFI010000205         5229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFFI010000205 DAZFFI010000000
VERSION     DAZFFI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.207"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..550)
                     /locus_tag="ACHJ12_004840"
     CDS             complement(<1..550)
                     /locus_tag="ACHJ12_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992499.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESV"
     gene            complement(552..830)
                     /locus_tag="ACHJ12_004841"
     CDS             complement(552..830)
                     /locus_tag="ACHJ12_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992500.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            966..1223
                     /locus_tag="ACHJ12_004842"
     CDS             966..1223
                     /locus_tag="ACHJ12_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4992501.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            1229..1528
                     /locus_tag="ACHJ12_004843"
     CDS             1229..1528
                     /locus_tag="ACHJ12_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4992502.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGVYHGGQSYESDFL"
     gene            1590..1943
                     /locus_tag="ACHJ12_004844"
     CDS             1590..1943
                     /locus_tag="ACHJ12_004844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992503.1"
                     /translation="MNKTICFLLVFAISGCVNPYNYREEQNVIISFKTNSPPQEIQEC
                     ILSEWQRTPLLATITSQKIGKYYSVLAVGDNADIYKLADGSTIIDFYSLRGVLDPTNG
                     KSKRIQGIKSCISTK"
     gene            complement(2050..2205)
                     /locus_tag="ACHJ12_004845"
     CDS             complement(2050..2205)
                     /locus_tag="ACHJ12_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992504.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(2503..3066)
                     /locus_tag="ACHJ12_004846"
     CDS             complement(2503..3066)
                     /locus_tag="ACHJ12_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992505.1"
                     /translation="MTQASLPGGFTIEEAKELHEDLVRSHISKALSGEKMKKKDLDAD
                     LCWIHGVIVQASWFVKASLEQNALTGNSQVIPDDGREQFETLIRFHADDKNHETLLLR
                     ANEGMNYRDPNVDLAWIFWKSSREHILPGKSDNNPALGDQVSELTMLVKRLASSLKSV
                     NKSSKLPDKAMDYLKRNGLISAEDILR"
     gene            complement(3061..3204)
                     /locus_tag="ACHJ12_004847"
                     /pseudo
     CDS             complement(3061..3204)
                     /locus_tag="ACHJ12_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3204..3647)
                     /locus_tag="ACHJ12_004848"
     CDS             complement(3204..3647)
                     /locus_tag="ACHJ12_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992506.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VVYMWDSERKDIDAPGYYRTEHLVFAESSVKQWGGRVVPLYTAPPAPIVPAVLERLRA
                     IVADPRALPRRKEWVSGQQYSYVLLENVEAMVDEACRAAMLQGDK"
     gene            complement(3649..3993)
                     /locus_tag="ACHJ12_004849"
     CDS             complement(3649..3993)
                     /locus_tag="ACHJ12_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4992507.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALSAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(3990..4229)
                     /locus_tag="ACHJ12_004850"
     CDS             complement(3990..4229)
                     /locus_tag="ACHJ12_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992508.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYINDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIHKGGNQ"
     gene            complement(4222..4425)
                     /locus_tag="ACHJ12_004851"
     CDS             complement(4222..4425)
                     /locus_tag="ACHJ12_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992509.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            complement(4531..4716)
                     /locus_tag="ACHJ12_004852"
     CDS             complement(4531..4716)
                     /locus_tag="ACHJ12_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992510.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            complement(4709..4891)
                     /locus_tag="ACHJ12_004853"
     CDS             complement(4709..4891)
                     /locus_tag="ACHJ12_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992511.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(4987..>5229)
                     /locus_tag="ACHJ12_004854"
     CDS             complement(4987..>5229)
                     /locus_tag="ACHJ12_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4992512.1"
                     /translation="ICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFNDLCAA
                     FVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
BASE COUNT         1254 a         1388 c         1148 g         1439 t
ORIGIN      
        1 atacagactc cggaagcgca tcagccacct gatgacaaac agcccaccag cataactctg
       61 ccagcgacag ttcccgctcc tgtgtgtcat tcattgcgtg acggatgaca tcaatcatcc
      121 aggctgccag attctgctga gcaagttgat cgagtgattc tgatgtctgg tcgcgcagct
      181 ggttgtcaca gtgccagcac aacaccatcg cgccggtacc gtaacggtga atgacggttt
      241 cgctgtgatg ataatcaccg tgtggccact ggcaggattt cacgtgacgt aataaccagt
      301 cagacagtgc accagcgcca cccgcagcac gaatcacccg ttcgttgctg aaaaatggca
      361 gtaatgtttt atcctctgcc agcggctgtc gaacggcagg aacgactccg gacggcagac
      421 cgcgcatgtt tttcggttcc ggttccacca atattctgcc gttatggaat gctgacattg
      481 attcacggcc cggcttaagg agcaccatcc cgagttccgg taccagaaca ggtcgaagta
      541 atacccgcac gttacctcca gatgcgctgc tggaatgtgc gggacggacg cggtgggcgt
      601 tcggaataag ggagcctgac ggagattatc cagtgacgac gatcgaagct gagggctttc
      661 tcaaactcat atccgcgcct gcggtaacac tggatcagcc attcggcctg ttcttcagtg
      721 catgggggat gctgtaacca gtcagatttg aatgcatgag aacgccgccc gtgcctgctg
      781 gcaggggcgg cggagttatc cgaattgtaa aatctggtat cgtgcgccat cggttgtctc
      841 tgctggcgca gcaggtgcca gttgttcagg ctggcgtgcg gcaatattgt atctgatttc
      901 tggtgtcgtc aacaggcagt gtgctatcat cgaatagtgt tctatcctac tccgtgaggt
      961 ttaccatgcg tacaacccaa caattcagca ttacattaac taacgagatg gctgacatgg
     1021 tgcgcgcccg tgtggcttcc ggtgcctatg cttcagaaag tgaggtcatt cgtgaagggc
     1081 ttcgcgcact gaatgagcgc gataaagcaa tcgaagcgtg gttaacgcat tcagccgccc
     1141 catctctcga ttctatccgc gaaaacccaa acaacggacg ctccatttca caggttcgcg
     1201 ccgcgattcg agccgggaag taatctgcat gacgtatgaa gtcatcatta cttctgaggc
     1261 cgaacagcag ataatcaacc tgcacagata tataacagag aaggcaggga gcgccattgc
     1321 tgacaattat gccaacgcgc ttcttgatta tcttgatggt ttttccacat tcccgcatcg
     1381 gggcaataaa cgcgatgata ttcgacaggg aatgcgggta actcacttcc gccacagaac
     1441 gatcattgct tttgccgttg atggcaataa agtctttatc gctggcgttt accatggagg
     1501 gcaaagttat gaatctgact ttttatagtt tatattttca ctaacaaaaa acttaaataa
     1561 ccaagaaata cttataaaag ggcttctaaa tgaataaaac aatttgtttt ctattggttt
     1621 ttgcaatttc aggatgcgtc aacccttata actacagaga agaacaaaat gttattatct
     1681 cgtttaaaac aaattctcca ccacaagaaa tacaagaatg cattctcagt gagtggcagc
     1741 gtacacctct cttagccact atcacgtctc aaaaaattgg caagtattac agcgtattag
     1801 cggttggaga taatgccgac atttataagt tggcagatgg ttctactatc attgattttt
     1861 attcccttcg aggagtctta gaccccacca atgggaaaag caagaggatt cagggaatta
     1921 aatcatgtat tagcaccaaa taatcatcag ttcgaaccca gaaacagcca aatagatttt
     1981 atctcaggtg cgttgaggat gcctgacaca tcagaggtgg cgagggattt ctccctcgcc
     2041 tggtcactct tacttctcag attcgtagtc tacgaagaca gcaacctccg tctggccggt
     2101 tcggattcgt acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa
     2161 acagatgacg atcagggcga ttaacatcgc cttttgctgc ttcatagtct gcttctcctt
     2221 gacctttcgg tccgtaagag gctaacctac gtttgtgaag catagattgg gcctcagatt
     2281 aatgttaagc gtcctgcaag acgcgtaatg ttaactgggg cttttctctg tctgccttac
     2341 ggcggcatgc ccgaggcaga cagcctcaag cacccgcagc acttctactt aactcttctt
     2401 tccccgcaaa ccgtttttat ccccagcggc aaatcgaata caccaccagc accagcgcca
     2461 ccgccattgc aattcctacc gccacgaatg cctcaggcca ggtcatcgta aaatatcctc
     2521 cgcgcttatc agtccgtttc gcttcaggta gtccatcgct ttatccggta gtttgcttga
     2581 tttattgacg ctttttaatg aactggctaa tcgcttgacc aacatagtta attcactcac
     2641 ctgatcgcct aatgctggat tattgtcgct ttttcccggc aatatgtgct ctcgactgga
     2701 tttccagaat atccacgcca aatccacatt tggatcccgg taattcatcc cttcgttagc
     2761 acgaagcaat aatgtttcat ggtttttatc atctgcatgg aacctgataa gtgtttcaaa
     2821 ctgttcgcga ccgtcatcag ggattacctg agagttaccc gttagcgcat tctgctccag
     2881 tgatgctttt acaaaccagg atgcctggac tataacccca tggatccagc acaaatcagc
     2941 atcgagatcc ttcttcttca tcttttcgcc acttaaagcc ttgcttatgt ggctgcgcac
     3001 caggtcttca tgtaactcct tcgcctcctc aatggtgaaa ccgccaggca gactagcctg
     3061 agttatcgga gagttgccag ccttgcgcat ggcaatctcc atgatttcaa ccatatcccc
     3121 tggtggaatt ttacaaagtt gcccaataca tttctgctgc ctggcatatt cgagaatgtg
     3181 ctccagtttg gttcgattaa tcattattta tctccctgaa gcatggctgc gcggcaggct
     3241 tcatcaacca tagcttctac gttttcgaga agtacgtaac tgtactgctg cccgctaacc
     3301 cattcttttc tgcgaggtaa tgcgcgtggg tccgctacaa tagctcgcaa acgttccaga
     3361 acagctggca ctatcggcgc tggcggggcg gtgtaaagtg gaacaactcg ccctccccat
     3421 tgcttaacac tactctcagc gaacactaaa tgctcagtcc gataataccc aggagcatca
     3481 atatcttttc tttcgctatc ccacatgtaa accaccggtt ctgcttccag cgatgccagt
     3541 gcgataagcg ccagctcacg aatttcaccg ccgtctatat cgtcaatgtc atcgcggcca
     3601 gaaatgttag ccagccattg cagtcgctct ttggtaatag tggtcatctc actctccttc
     3661 tatgcgaata ccagcagcag tcagtgctgc attgacctca tcagcgtagt agtacgttaa
     3721 tccacttgat gatttagcca gcttgaatgg ttctggtaac ttcactgtgc gggactcctg
     3781 ctcgtcgata cgctgttttg cttcctccag ttcatccagc aaatcagcga taatatccac
     3841 ttcacgatga cggatttctc gcttaaacgc agacaaagct gcatcacaat cctgctcagc
     3901 gtttggactg tctggtttct cctgatacca cgccagcatc gaccgatagt tttgtgctgc
     3961 ctcacgcaat gcctgatagt caatcttgct cactggttgc ctcctttgtg gatctgcgct
     4021 gcgatgcacg aaaaaaaaga ctcccgcgta tgactgttaa gagctggtgc gaacgccgcg
     4081 ttaagaacgg cagcatcaca gccgtcattg atatagagcg caattttttt ctccaggcgc
     4141 gctttggctt cctgcaactg cataccccgg cacgcacgcg ggatatactc agcaatttga
     4201 gcgatagatt tttcgttctg tttaaacatg cttcacctcg acaggcttga tggtgtcgat
     4261 cagtagtcgg cggcgcgtat tttctgcaaa gtggcggcgt ccggtttctt tgtggtaaaa
     4321 ctcgtttttg tcgacgaccc acatccgctc cgtctggtgc agttttttta cctgcggacc
     4381 gtctttggtg atcacgatgc cggtatgggt ttttacgatt gtcatgccac cacctcttcg
     4441 aatttcaatt ccaattgttc accccagatt tcacatgact ctgaacacga gccggtatcg
     4501 aatcgcctgg cctgcaccat cgcctgatag ttaatcttca tcgtcctcac cccactcatc
     4561 acaataagct tcaaccggag tttttcctgc ttcatagtca tcacgccatg cttcagcatc
     4621 ggcggcactt ccaccgcgta actctgcata gtccattaac agttcatgcc attcttcaaa
     4681 actggcgtta tatttagttg aaccaaaatc agccattttg ttcttcctct tcgtctttta
     4741 tttcgtgata tgagtaattg cagtagttaa agaaaatttc ttttgcttcg tcatgaattt
     4801 catcaggtgt tgcgtcatcg tccacttcga atacatcctc aaaatctcca ccggctattc
     4861 ccgtttcaat aattattttg aactttcgca tttcactacc gccctgccgg gcggtctcct
     4921 gatgttctga gggtgcagga atccctccgg ttaaggatta aattttattt acagtgctaa
     4981 atttaattat tcaggtgcgc gaagttgttc tgcacaatgc agcaatgctt ctgtcgcttc
     5041 cttaaacgtt acggtatcgg catcatccag tcctgcaact tttgcgtgcc tgacaaacgc
     5101 cgcgcaaagg tcgttaaaca cctccgctcg tacatcagcc aggaaagcat cggtggctgg
     5161 catatttcct gttgccttca tggcctccaa aataaccaga acgccatctc tcccaacctc
     5221 ctcgcaaat
//
LOCUS       DAZFFI010000206         5222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFFI010000206 DAZFFI010000000
VERSION     DAZFFI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.208"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..1250
                     /locus_tag="ACHJ12_004855"
     CDS             111..1250
                     /locus_tag="ACHJ12_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFO4992513.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            1370..2638
                     /locus_tag="ACHJ12_004856"
     CDS             1370..2638
                     /locus_tag="ACHJ12_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4992514.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            2646..3791
                     /locus_tag="ACHJ12_004857"
     CDS             2646..3791
                     /locus_tag="ACHJ12_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO4992515.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            4259..4405
                     /locus_tag="ACHJ12_004858"
     CDS             4259..4405
                     /locus_tag="ACHJ12_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992516.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(4476..>5222)
                     /locus_tag="ACHJ12_004859"
     CDS             complement(4476..>5222)
                     /locus_tag="ACHJ12_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO4992517.1"
                     /translation="TIRVDFQHKNDIEEAFKYASINNMVPISISDSVGSMGGVAPVEF
                     IVRMANRFDGYAYIDDAHGMSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGG
                     FIACNYPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIK
                     YFDDKFNETEVINKGTTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKA
                     ILRCAISAIHTRENIDSLHKNFIECINEQG"
BASE COUNT         1272 a         1308 c         1309 g         1333 t
ORIGIN      
        1 gccatacttc ccgctttctt tgtctgttta cacggaacat ttcaccactg gatacgctaa
       61 tagatcggat atgagcacat attgcgcccg cctttcatgc agagcataaa atgaacaaga
      121 ttcacgctaa ttattacctg caagattcta ccgcacgcca gatagttcaa cgcacaatgg
      181 ggattattcc gtactcggta aatgtgatga acgagcatgg gatgatcatc gcttccgggg
      241 aagcctcacg tttacatcaa cgccatgaag gtgcggtact cgcattgacc gaaaaccggg
      301 tcgtcgaaat tgattccgcg accgccaata agcttaaagg cgttaagccc ggaatcaatt
      361 taccgattaa ttttcgcgac cagctgattg gtgtactggg cattaccggc gagcctggtg
      421 aagtgaaggc atatgcacag ttggtgaaga tggcttcgga attaatcatt gaacaaatgg
      481 ctctcctcga gcaaaaacag tgggataagc gttaccgcga agagctgatt aatcagctga
      541 ttttacgcga tccgccaccg gactcactga atgcggtggt ctcatggttg ggaattaatc
      601 tccagcttcc gcgggtggtg gtgattatgg aactgcaaca acctgatcgc gacgcactgc
      661 ataatctgat ggagtatttt gaatatcgcg cccgcgacca tttggtcacg ttttccgact
      721 ttaatgatct gataatctta aaacctatct cgctaaaaaa cggcgtctgg gataaatcgt
      781 tggaattaag cgatctgcag agacttaaac attacgccca gtcctgtggt ttttcccgac
      841 tgattgtcgg cggctatttc tctggagaaa acggcctgca tcgctctttt cagaccgcga
      901 aatccaccca gtccatggcg aaaagactga agttgaagca tcagttcatt tttcatgacg
      961 actatgcgct ggcatcatta ttagatgggc tttctggtac ctggcaagcg gaagaattat
     1021 ccaaaatctg gcgcaatctg gttgccaagg acaccaaagg tatgctgcaa aaaacgctgc
     1081 agaattattt tgatcataat tgtgatcttt cgcaaacagc gactggcttg catattcatg
     1141 tcaatacatt acgctatcga ctgcagagag tggaagatat tacaggaata caaatcaata
     1201 acttaaagca gttattctgg ctgtatatcg gcatggagtt acaacgctga actgtatttc
     1261 cctccaaatt tttcgccgtc tgacgtcgta tttttagccg atacacacaa gtctaacgcg
     1321 gctgctttct ccatactgcc ccctgttatt ttcatggaga aagcagaaga tggtttcaat
     1381 aacagcaact ggcgccatca tcgcactcgt ggtggcgatc gttttaattt tacgcaaagt
     1441 ctcgccggct tacggcatga ttgtcggtgc gctggtaggc ggtctggtcg gcggcgccga
     1501 tctggtgcaa accgtgacgc taatggtcac tggcgcgcaa ggcattacca atgccgtgct
     1561 gcgcattctc gcagcaggtg tactggccgg cgtgttgata gaatccggcg cggcaaacac
     1621 catcgctgaa actgtggtga aaaaagtggg ggaaagccgg gcgcttttag ccttagctat
     1681 tgcgacgatg attctgaccg ccgtcggagt atttatcgac gtggcggtta tcactgttgc
     1741 gccgattgcc ctgtctattg cccgtcgtgc cggtttgtcg aaactcgcca ttctgctggc
     1801 gatgattggc ggtggtaaag cgggtaatgt tatgtcgcct aaccccaatg ctatcgccgt
     1861 ttcggacacc tttcatgttc ccctcacctc cgtgatgatg accgggataa tccccggtct
     1921 gttcggcctg atcgtggctt atttcctggc aaaatgtctc agccagaaag gcctgatggt
     1981 gatgcccgac gaaatgattt cacacgacaa ccacggcaag cagccgggat ttcttgccgc
     2041 tatcagcgcg ccgctcgtcg ccattgtgct gctggcgcta cgacctgttg cgggtatcgt
     2101 catcgatccg ctgattgccc tgcccgtcgg tgggttagcc ggtgcgatcc ttatgggcag
     2161 gtttaagcaa agtaacgcct ttatgatgtc cgggcttacg cgcatggcgc cggtagccat
     2221 catgctgctc ggtaccggta cgctcgccgg gattatcgcc aactcggaac tgaaagatgt
     2281 gctcattcat ggactgacct cttccggtct gccttcttat cttctggccc ctgtttccgg
     2341 cgcgatcatg tccatggcaa cagcctccac cacggcggga accgctgttg ccgccggggt
     2401 attcagccag acgctgctgg ggcttggcgt atcagccctt gccggttccg ccatgattca
     2461 tgctggcgca accgtacttg atcacctgcc tcacggcagc ttcttccacg ccacgggcgg
     2521 cagtgtcggc atgctgatcc atgaacggtt aaaactgctg ccttatgaaa cccttgtcgg
     2581 ttttgtggtc gccctgattt ccacactgat gtttggcgtc ttcggacttg ccgggtaaag
     2641 acacgatgaa aatagtcatt gcaccagatt catttaaaga aagtttatcg gccatgcagg
     2701 tggccaccgc tattgagcag ggatttcgtg aaatttatcc cgaggccgag tatgtcaaat
     2761 tgccgatggc tgacggcggt gagggtaccg tcgaatcaat ggttgaagca accgaaggcc
     2821 attatctgtt ccagaatgtc accggaccgc tggggacgcc agtacaggcg cgctggggta
     2881 tgctgggaga tgggaaaacg gcgatcgttg aaatggcctc cgcatccgga ctacatcatg
     2941 ttcctccaga atgccgtaac cctatgctta ccaccagtta tggcacaggc gaactgatcc
     3001 ttgccgcact ggaccacggc gttgagcgaa ttattctcgg gattggcggt agcgccacca
     3061 atgacggcgg cgcaggtatg ctacaggcac ttggtgtggc gatgcttgat acaaacggga
     3121 aatcgcttcc tttcggcggc ggcgcgctaa gcaacctggc gctcatcgat gcctcaggct
     3181 gcgacgctcg actgaaaaac gtttctgtca ccgtcgcctg cgacgttaac aatccactct
     3241 gtggcgcaac aggagcgtca gccatcttcg gcccgcaaaa aggagcaaca ccagaaatgg
     3301 ttgcctcctt agaagctgcg ctgaccaact tcggtatacg acttcaggac gcaaccggac
     3361 gaaacgtgat gacgacgcct ggcgcaggtg ccgctggcgg catgggcgcg gcgctgttag
     3421 ggattctgaa tgcggagtta aagccgggag tggagattgt tattgacgcg ctgcatctcg
     3481 atagcctggt cgcggatgcc agcctggtaa tcaccggtga aggtcgtctt gatagccaga
     3541 gcatcagtgg taaaacaccg attggtgtcg cacgctgcgc taagcggtat cagaaaccgg
     3601 tgatcgcact ggcgggcggc ttaacgaccg accaccagat agtgcatgag cacgggctgg
     3661 acgcggtgtt ctccgtgctg acgcaaatcg ccactctgcc ggaagcgcta caggatgcgg
     3721 aatacaacct ccgcgtttcg gcacgcaata ttgccagcgt ctggaaatta tcacagcagc
     3781 agatgcgcta atcttcagtc aggccggcaa gcagtagctg gcctggatat tctttctcac
     3841 caggcccatg ctaaaacagc ctgcttcagt catcccacgc aaggcactag catggcaaaa
     3901 aaagagggga gtagtggatc ccaaaagacg gttaagcggt taaattgaat ggtcaataag
     3961 ggggctgttt gggaaacgaa tctatggtca ctcccgtttt tgcaacaccg attttgacga
     4021 taagtgggct tgcttgaatc tatccggcgt ctgaatggga ttttattccc gcgcctcgat
     4081 gagttccgcg cctgatgaac ctcaagaaaa tatacggctt caatgagcct ttccgtttca
     4141 caggttcctc aacaggccgg tgggccgttc atatcatcaa taccgtgatg ctgatactcc
     4201 gtacccaccg gaaccgcacc tgatgcggtt ccggcttaag ggctgtcagg actcgctttt
     4261 ggctgccatc aacaaaccgg aagagatgcc ggattatctc ttccgtcaac tcggttttaa
     4321 tcagacctgg catgagtgga agcgggacga acagcacagg caacaacaac gccggcccgg
     4381 acactaccgg ggcatgagta tgtaatgtca gggacagcta tacgccataa aaaaatgaaa
     4441 tagatggcct ctctataaag aggccatgat atttactaac cctgttcgtt aatacattct
     4501 ataaagtttt tatgtaggct atctatattt tctcgggtat gaatagctga tattgcacaa
     4561 cgtagaattg cctttccctc ttccactgta ggatacatag caacggttgt agcaaaacca
     4621 cgatcatgca tttttttgca tatcgttata gctttttctt cattgccaat taacaatcca
     4681 cgtattggca ttgtagtccc tttgtttatg acttcggttt cattaaattt gtcatcaaag
     4741 tattttataa cttgttgtaa gtcattttga cgcaaggata ctgtgccatc gaggtgtaaa
     4801 tctgctgctg cgatacatgc agcaattcct ggtaatgatg gtggaccacc aaaagcatag
     4861 gtagttccat acgtttttat aaagtttatg gtgtcaggat aattgcatgc aataaatcca
     4921 ccatgagagc caaacgcttt agataatgaa ccagcgataa taattctgtc atcaagtttg
     4981 ttatccagac aagacattac atatccggag ccatttttcc catatattga catgccatga
     5041 gcatcatcaa tgtaagcata cccatcaaat ctgttagcca ttctaacaat aaattcgaca
     5101 ggagccaccc cacccatcga accgacacta tcagatatag atattggtac catattattt
     5161 attgaagcat atttaaacgc ttcttctata tcatttttat gttgaaagtc aaccctgatt
     5221 gt
//
LOCUS       DAZFFI010000207         4857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFFI010000207 DAZFFI010000000
VERSION     DAZFFI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.209"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..1993
                     /gene="mngA"
                     /locus_tag="ACHJ12_004860"
     CDS             77..1993
                     /gene="mngA"
                     /locus_tag="ACHJ12_004860"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO4992518.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGDTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVRVAEPIRHAEALMQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILVFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2011..4644
                     /gene="mngB"
                     /locus_tag="ACHJ12_004861"
     CDS             2011..4644
                     /gene="mngB"
                     /locus_tag="ACHJ12_004861"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO4992519.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLMLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4669..>4857
                     /locus_tag="ACHJ12_004862"
     CDS             4669..>4857
                     /locus_tag="ACHJ12_004862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992520.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIF"
BASE COUNT         1189 a         1137 c         1340 g         1191 t
ORIGIN      
        1 tgaaattgat cacataatgg tattgtttta tcgggcacac tggcgcgact ataaaaacga
       61 tcaagtgagg atcatgatga atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa
      121 tgcgcgcttt accagccgtg aagaggccat ccacgcgttg actcaacgtc ttgctgctct
      181 ggggaaaatt tccagtactg agcaatttct ggaagaagtg tatcgccgtg aaagccttgg
      241 cccgaccgcc ttaggtgaag ggttggctgt gccgcatggc aaaactgctg cggtaaaaga
      301 agcggcgttt gcggtcgcca cactcagcga gccgcttcag tgggaaggcg ttgatggccc
      361 ggaagcggtt gatttagtgg tgctgctggc gattcccccc aatgaagcgg gtactacgca
      421 tatgcaactg ctgacagcgc tgaccacgcg cctggcggat gatgagattc gggcgcgtat
      481 acagtcggcg acgacgcctg atgagttgct ctcggcgctg gatgacaagg gagacacgca
      541 accttctgcc tctttttcca acgcgccaac tatcgtctgc gtaacggcct gtccggcggg
      601 tattgctcac acctatatgg ctgcggaata tctggaaaaa gccggacgca aactcggcgt
      661 aaatgtttac gttgaaaaac aaggcgctaa cggcattgaa gggcgtttaa cggcggatca
      721 actcaatagt gcaaccgcct gtatttttgc ggctgaagtc gccatcaagg agagtgagcg
      781 ttttaatggc attcccgcgc tttcagtgcg tgttgccgag ccgattcgcc atgcagaagc
      841 gttgatgcaa caagcgctta ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga
      901 tacgcaaccg gtgaaaagtg tcaaaacgga gctgaaacag gcactgttga gcggaatctc
      961 ttttgccgta ccgttgattg tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc
     1021 gcaaatcttc gggctacaag atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg
     1081 caagctgggc ggcgggctgc tcggaatttt gatggtaccg gtgctcgcgg cctataccgc
     1141 ctattctctg gcagataaac cggcgttagc gccaggcttt gcggctggac ttgccgccaa
     1201 catgatcggc tccgggtttc tcggcgcggt cgttggcgga ttgatagccg gttacttgat
     1261 gcgctgggtg aaaaatcact tgcgtcttag cagtaaattc aatggattcc tgacttttta
     1321 tctctacccg gtgctcggta cgttgggagc gggcagtctg atgctgtttg tggtgggaga
     1381 acctgtcgcc tggatcaata actcgcttac cgcctggctg aacggtctgt caggaagtaa
     1441 cgcgctgttg ctgggtgcca ttctcgtttt tatgtgttcc tttgaccttg gagggccagt
     1501 gaacaaagcc gcttatgcat tctgcctggg cgcaatggcg aacggcgttt acggcccgta
     1561 tgccattttc gcctccgtca aaatggtttc ggcatttacc gtaaccgctt ccacgatgct
     1621 cgcaccgcgc ctgtttaaag agtttgaaat tgagaccggg aaatccacct ggctgttagg
     1681 gctggcaggt attaccgaag gggcgatccc gatggcgatt gaagatccgc tgcgggttat
     1741 tggttcgttt gtgctgggct ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg
     1801 cctttcgaca cccggtgccg gcattttctc gctcttttta cttcatgata atggcgcggg
     1861 cggtgttatg gcggcaattg gctggtttgg tgcggcattg gtgggggctg caatctcgac
     1921 tgcaattctc ctgatctggc ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg
     1981 cgtaatgcca taaacaaaaa ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc
     2041 ccgcatatgc attgggatcg ggagtggtat ttcaccaccg aagagtcacg cattctgctg
     2101 gtcaataata tggaagagat cctgtgccga ctggaacagg acaacgaata caaatattac
     2161 gtactcgacg ggcaaacggc gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa
     2221 gaccgcgtga agaaacaggt agaagccggt aagttgatta tcggcccctg gtatacccag
     2281 accgatacca cgattgtttc tgcggaatcc atcgtccgta atctgatgta cggaatgcgt
     2341 gactgcctcg cgtttggcga gccgatgaaa ataggttatt taccggattc ctttggcatg
     2401 tccgggcaac tgccgcatat ctacaatgga tttggcatta cccgcactat gttctggcgc
     2461 ggatgttcgg agcgccacgg tactgataaa accgagtttt tatggcaaag cagtgacggt
     2521 agcgaagtga cggcgcaggt gctgccgttg ggttatgcca tcggtaagta cttacctgcc
     2581 gacgaaaacg gattacgcaa acgcctcgac agttattttg acgtgctgga aaaagcgtct
     2641 gtaaccaaag agattttatt gccgaatggt catgaccaga tgccattgca gcaaaatatc
     2701 ttcgaagtga tggagaaact tcgcgaaatt tatcctcaac gaaagtttgt gatgagccgc
     2761 tttgaagagg tattcgagca gatcgaagcg cagcgtgaaa gtctggccac cctgaaaggt
     2821 gaatttatcg atggcaaata tatgcgcgta catcgcacca tcggttccac gcgcatggat
     2881 atcaaaattg cccacgcacg tattgaaaat aagattgtta atctgctgga accgctggca
     2941 acgctggcct ggacgttggg ttttgaatac caccacggct tgctggagaa aatgtggaaa
     3001 gagatcttaa aaaatcatgc ccacgacagt atcggttgtt gttgcagtga caaagttcat
     3061 cgcgaaattg tcgcccgctt cgaactggct gaagacatgg cggataatct gctgcgtttc
     3121 tacatgcgca aaattgccga taacatgcca cagagtgatg ccgacaaact catgctgttt
     3181 aacctgatgc cctggccgcg ggaagaagtt attaacacca ctgtgcggct gcgcgccagc
     3241 cagtttaatc tacgagacga tcgcggtcag cctgtaccgt attttattcg ccatgcccgt
     3301 gagatcgatc caggcctaat cgatcggcaa atagttcatt acggtaatta cgatcccttt
     3361 atggagtttg atatacagat caaccagatt gtcccttcta tgggctatcg cacgctttat
     3421 atcgaagcga atcagcctgg caacgtgatt gcggcaaaaa gtgacgctga agggatactg
     3481 gaaaatgctt tctggcaaat tgcgctcaat gaggatggaa cgctacaact ggtagataaa
     3541 gatagcggtg tgcgctatga ccgggtgttg caaattgaag aaagctctga tgatggtgat
     3601 gaatatgact attcacccgc aaaagaagag tgggtaatga cttctgcaac agcgaaaccg
     3661 caatgcgaga ttactcatga ggcctggcag agcagggctg ttatccgcta tgacatggcg
     3721 gtgccgctca atttgttgga acgaagcgtc cggcaatcca cgggcagagt aggggtggag
     3781 atggttgtca ctctgagtca taacagcaga cgtattgatg tggatatcaa ccttgataac
     3841 caggctgacg atcatcgcct tcgcgtcctg atccctacat cttttaacac cgatagtgtt
     3901 ctggcagata cccagtttgg ttcgctaacg cgccccgtga acgacagtgc aatgaacaac
     3961 tggcagcaag aaggctggaa agaagcgccg gttccggtat ggaatatgct caactatgca
     4021 gccttacagg aagggcgtaa cggcatggct gtctttagcg aagggctacg tgaatttgaa
     4081 gtcatcggtg aagagaagaa aacctttgcc attacgttgc tgcgtggcgt gggcttactg
     4141 ggcaaagaag atctgttttt aaggcctggg cggccttcgg gaattaaaat gccagtcccg
     4201 gactcacaac tacgtggtct gctttcttgt cgcctaagtt tattgagtta taccggtacg
     4261 ccaaccgccg ctggtgtagc tcagcaggcg cgagcatggc tgactccagt acagtgttac
     4321 aacaaaatcc catgggatgc gatgaagctc aacaaagccg gattcaacgt gccggaaagt
     4381 tatagtttgt tgaaaatgcc cccagtggga tgcctgataa gcgcacttaa gaaagctgaa
     4441 gaccgacaag aagtgatttt acggctgttt aatccggctg aatcagcaac ctgtgatgcg
     4501 actgttgctt tcagtcgcga ggtgatttct tgctcagaaa cgatgatgga tgaacacatt
     4561 accaccgagg aaaatcaagg ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg
     4621 acgttcagtt accggcttgc ctgaatagca atcaaaccga agccacatat gcgcggccag
     4681 attgttgaca aagggcgctt tgttcatgcc ggatacggca tgaacgcttt attcggtcta
     4741 caaaagcagg caaattcaat atattgcaga gatgatgtag acactggcaa gcgtagcgca
     4801 tcaggtaatt ttgcgtttat cttcactctc aagccacgta tatgtggctt tattttt
//
LOCUS       DAZFFI010000208         4845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFFI010000208 DAZFFI010000000
VERSION     DAZFFI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.210"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            193..1167
                     /gene="hemB"
                     /locus_tag="ACHJ12_004863"
     CDS             193..1167
                     /gene="hemB"
                     /locus_tag="ACHJ12_004863"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4992521.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1273..2124)
                     /gene="tauD"
                     /locus_tag="ACHJ12_004864"
     CDS             complement(1273..2124)
                     /gene="tauD"
                     /locus_tag="ACHJ12_004864"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4992522.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2121..2948)
                     /gene="tauC"
                     /locus_tag="ACHJ12_004865"
     CDS             complement(2121..2948)
                     /gene="tauC"
                     /locus_tag="ACHJ12_004865"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4992523.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2945..3712)
                     /gene="tauB"
                     /locus_tag="ACHJ12_004866"
     CDS             complement(2945..3712)
                     /gene="tauB"
                     /locus_tag="ACHJ12_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4992524.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3725..4687)
                     /gene="tauA"
                     /locus_tag="ACHJ12_004867"
     CDS             complement(3725..4687)
                     /gene="tauA"
                     /locus_tag="ACHJ12_004867"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4992525.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4665..>4845
                     /locus_tag="ACHJ12_004868"
     CDS             4665..>4845
                     /locus_tag="ACHJ12_004868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992526.1"
                     /translation="MLRDEIAMIVCSPVLLWEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWE"
BASE COUNT         1122 a         1393 c         1237 g         1093 t
ORIGIN      
        1 taataaccaa gagctataaa taaagattaa aaatattttc agtgaatatg aaattattgc
       61 tatttctatt acaaagatgt cctggtctct ctttcattct cttcttgtca acatcacgac
      121 aactttcgta aaacactcct gccccgcttc aggtatactg tgcaccttaa ttccacaaac
      181 atcaggcaga ccatgacaga cttaatccaa cgccctcgtc gcctgcgcaa atctcctgcg
      241 ctgcgcgcta tgtttgaaga gacaacactt agccttaacg acctggtgtt gccgatcttt
      301 gttgaagaag aaattgacga ctacaaagcc gttgaagcca tgccaggcgt gatgcgcatt
      361 ccagagaaac atctggcacg cgaaattgaa cgcatcgcca acgccggtat ccgttccgta
      421 atgacttttg gcatctctca ccataccgat gaaaccggca gcgacgcctg gcgggaagat
      481 ggactggtgg cgcgtatgtc gcgcatctgc aagcagactg tgccagaaat gatcgtcatg
      541 tcagacactt gtttctgtga atacacttct cacggtcact gcggtgtgct gtgcgagcat
      601 ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc aagccgtggt tgcagctgct
      661 gcaggcgcag acttcatcgc cccttccgcc gcgatggacg gccaggtaca ggcgattcgt
      721 caggcgctgg acgctgcagg ctttaaagat acggcgatta tgtcgtattc gaccaagttc
      781 gcctcttcct tttatggccc gttccgtgaa gctgccggaa gcgcattaaa aggcgaccgc
      841 aaaagctatc agatgaaccc aatgaaccgt cgcgaggcga ttcgtgagtc actgctggat
      901 gaagcccagg gcgcagactg cctgatggtt aaacctgctg gagcgtacct cgacatcgtg
      961 cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt atcaggtgag cggtgagtac
     1021 gcgatgatta agttcgccgc gctggcgggt gctatagatg aagagaaagt cgtgctcgaa
     1081 agcttaggtt caattaagcg tgcgggtgcg gatctgattt tcagctactt tgcgctggat
     1141 ttggctgaga agaagattct gcgttaattt ttttcctctc agaaaggggg agttaacctg
     1201 ttgaaagtac ggtgaacgcc ccctcaccct aaccctctcc ccaagggggc gagggaaccg
     1261 tccgcactcg tatcaccccg cccgataaaa cggtttatcc ccaagaatcg tcgcccgatg
     1321 cataatccgt cgctgtggca ggtaatcggc attggcatag tgctgggtca cgcggttatc
     1381 ccaaatcgca atatcatttg gttgccagcg ccagcgcacc tgaaactccg gtttagtgat
     1441 atgagcgaat aaaaaactta acaaggcttc gctctctttc tcgctcacat caacaattcg
     1501 cgtagtaaag ccttcattca caaacagcgc ctgtttaccg ctcaccggat gcgttcgcac
     1561 caccggatgt agcaacggcg gatttttcgc gaccgcctcg cgccagcgtt gatgttcctc
     1621 ctcggttttg cggtatttgt attccgggaa cgatttacgg aaatcatgct ccgcacgcag
     1681 cccgctcagc agctggcgga agggaacaga gagcgcctca taggccgcaa taccgctggt
     1741 ccagagcgta tcaccgccgg tcgaaggtaa ctctttagct gccagaatcg cccctgcggg
     1801 tggcgtttca ataaatgtca catcggtatg ccagttgtcg ttatccggcg gattatcgtt
     1861 atgggtatcc agcacgatga tctcgtcaac cccttcggca tgagggtaaa cagggtgaat
     1921 atgcagttcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgtaa tagcttgatc
     1981 gcgcagaaat accacctgat ggcgcagcac cgcatggtaa agctgttcaa actgattatc
     2041 gcttaacggg cgcgtcaggt cggcacccga aatttgtgcg ccgatatacg gccccagcgg
     2101 ggtaatactc agacgttcac tcattgtact tctccatgcc agggcgtcag acggcgctgt
     2161 aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc gatccccgcc
     2221 agtaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc taaaccgcgt
     2281 gtcgcggcaa tcagctccgc cgctaccagc gtagaccagc ccacacccag accaatgcgt
     2341 aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag cacctgcgca
     2401 cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct tttcaccccc
     2461 gccagcgccg acatcgccac cggtgcaaaa atcgctaaat agatcagtaa gatcttcgag
     2521 gtttcaccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg cggcaccgga
     2581 cgataaagtt cgattaccgg atccagaatg ccgcgcaccg tcgggcttag ccccatggcg
     2641 atcccgaccg gaataccgat caccaccgcc gcgagtagcg ccagcacaat gcgcgtcagg
     2701 ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg
     2761 agcagttttg ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat cagttgcagc
     2821 gccgccaccg tccaccatac agtgagtaaa accgctaacg tgccaatgct taaggtcacc
     2881 tgacgcgaga gcggccagcg ccatttcagc cgccgcgaat gcagtttttc attaatgagc
     2941 acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat tcgcgcatgg
     3001 cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca aagcggcgag
     3061 caaagttgag cggcagccgc tccagcacac ggccagggcc ggatgaaagc agaaccagtt
     3121 cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc tgcttgcccg
     3181 tctcctgcca gagtttcagc agcagagttt gcatctggtc gcgggtgaag gcgtccagcg
     3241 caccaaacgg ttcgtcgagt aataacagct ggggattcgc cgccagcgcg cgggcaatcc
     3301 ccacccgctg acgttgacca ccggaaagct gccagatata gcgtttttct gcgccttcca
     3361 gccccacttt tttcagcatc tggtgcgcga tttccagtcg ctgcattttc tctatacctg
     3421 ccaattgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtagt agcccttcat
     3481 tctgaaaaac caccccgcgc tctgctcccg gtccctcaat acgcttaccc gccagttgaa
     3541 tgctgccatg ctgataaggc acaaaaccgg caatcagatt cagcagggtg gttttaccgc
     3601 agccggacgg ccccagcacc accagtagct cgccgctttc cagcgtcagg ttgatatctt
     3661 ccagtaccgg tttgccgcca taatcggcgt aaagatgaga gatttgcagc atctgcgcct
     3721 ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct acagccggga
     3781 ccttgccctg ctctttcaaa aactgcgcgg tgtcgatgat cgctttgttc accggtccgg
     3841 tcagttctac cgtttgttgc tgcggcgtca gataggtatt ccccttcacc agccccggaa
     3901 cgtcaccttc cggcacgccg cttaaacgcg ccagtttgct gatgttttcc ggctgtttca
     3961 gccacgcgtc tgggttagca atgtacggtt gctgagcatc gatggcgctt ttagcgaacg
     4021 ctttcacgac ctcaggatgt ttctcggcaa aatctttgcg caccacccag acgtccagcg
     4081 ttggcgcacc ccactgcccg acctgttcag aatcggtcaa caccttgccg tctttttcca
     4141 gggcgttaac cgccggtgcc cagacataag caccatcaat atcaccccgc tgccatgcgg
     4201 cgataatcgc gggcggctgc aggttcacaa tctccacttg ccctggttta atcccccagt
     4261 gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg gcaatgcgtt
     4321 tgccaatcaa atcttcgggt ttgctgatag ttttctttac caccagcgct tcggagttac
     4381 ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggct gcaaccgcta
     4441 atgggctgga accgaggttg ccgatttgca cgtcgccaga agccagcgcc cgcacgatgc
     4501 tggctccgct gtcaaactta cgccagtcca cggttgctcc gctttcttta gcaaaggtgt
     4561 tgtcggcctg agccactttc gccggttcgg ctgaggtttg atacgccacg gtgacgttca
     4621 ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta cgcgatgaaa
     4681 ttgccatgat tgtctgctcc cctgtcttgt tatgggagca gtattcagga ataaaaacat
     4741 tcattaaaag aattagtcgt tatcgcacag atgattttat tcttagcaaa aaaacggcta
     4801 aggaaagatt aaatatgcaa ttactccgtt ctaacaattg ggaaa
//
LOCUS       DAZFFI010000209         4762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFFI010000209 DAZFFI010000000
VERSION     DAZFFI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.211"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            362..555
                     /locus_tag="ACHJ12_004869"
                     /pseudo
     CDS             362..555
                     /locus_tag="ACHJ12_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            597..926
                     /locus_tag="ACHJ12_004870"
     CDS             597..926
                     /locus_tag="ACHJ12_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4992527.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            907..1188
                     /locus_tag="ACHJ12_004871"
     CDS             907..1188
                     /locus_tag="ACHJ12_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992528.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            1371..1655
                     /locus_tag="ACHJ12_004872"
     CDS             1371..1655
                     /locus_tag="ACHJ12_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO4992529.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            1655..1930
                     /locus_tag="ACHJ12_004873"
     CDS             1655..1930
                     /locus_tag="ACHJ12_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4992530.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(2196..2692)
                     /locus_tag="ACHJ12_004874"
                     /pseudo
     CDS             complement(2196..2692)
                     /locus_tag="ACHJ12_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2969..3844
                     /locus_tag="ACHJ12_004875"
     CDS             2969..3844
                     /locus_tag="ACHJ12_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO4992531.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLAAGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            3852..4628
                     /locus_tag="ACHJ12_004876"
     CDS             3852..4628
                     /locus_tag="ACHJ12_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO4992532.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT         1290 a         1174 c         1122 g         1176 t
ORIGIN      
        1 ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag cggcgggccc cgaccggagc
       61 cattttagtt acaacactca aatacaagca ccgggaaaac cccggtccag cgcagaaccg
      121 aaaccgcaaa gcccctccct cataacttaa aagcggcccc gccccggccc aaagggccgg
      181 aacagagtcg cttttaatta tgaatgctgt aactaatcat cttcatcgct gtcagtcttc
      241 tggctggaag tcctcagtac acgctcgtaa gcggccctga cggcccgcta acgcggagat
      301 acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca taagctctat
      361 catagctgac agcgggtatg gctttgcagg gtggggatgg gtaaggtgaa atctatcaat
      421 cagtaccggc ttacgccggg cttcggcggt tttactccgg tatcacatgt aaccacagcg
      481 tgccgcctta catgcgctga cgcgcataaa caacaagttg ctacattagc ttacaggtta
      541 attcaccttg cataagatta gcatataggt taatgtttat cgaaatggga ggtcatatgt
      601 ttagcgttgt ttatcacccg gaagccaggg aagaagccac agcattaccc ataaaaatca
      661 gggtgaagtt tgaccgtctc atcagcaaac tggaatatga cgcccgatta ctgcgggaac
      721 cggatacaaa gcccctgggg gatggacttt ttgagatccg cacgatgggg acggacatag
      781 ccagaggcat ctgggtatac cacaaaggca ataccatcat catgctccgg gttttcatca
      841 agaagtctca gaaaacgcca gcaaaagaaa tcgatctcgc caaaaagcgg ttagcggagg
      901 tacttaatga aactggtaag ccataaagaa ctgcataacg agtggatgca ggacgacgaa
      961 tatcgcgctg catggcagga agaagaacgt aaggagaaac tcagggagtt gcttgcagaa
     1021 tggcgtaagc atgacaattt gacaaaagcg caggttgctg aacgcatggg ggttactcct
     1081 cctgttattt cccgcctgga aaacaacatc actaaagcca gcatcgacac cctgactcgc
     1141 tatgcccatg cgtgcggtat caaaaaacct gttattacgc tgtactgaac tctgctctat
     1201 ggctgccagt tctggagaaa tcagtaacta aattttaatt agctacgtta tggtaaatct
     1261 gcctgatgtg tcgatttctc tgaaagtaat tcggcttctg tgggctgcgt ttgccttgtg
     1321 ccacctgaat gtaatcacta tgattaaata tgattaacaa ctaatcggat atacaaatga
     1381 aaaacaatac cgcacaggca acaaaagtaa ttaccgcgca tgtgccatta cctatggctg
     1441 ataaagtcga ccagatggcc gccagactgg aacgctcccg gggctgggtt atcaaacagg
     1501 cactttctgc atggcttgcc caggaggagg agcgtaatcg cctgacgctg gaagccctgg
     1561 acgatgtgat atccggacag attatcgacc atcaggctgt acaggcctgg gcagacagcc
     1621 tcagtactga caatccgtta ccggttccac gctgatggaa ctgaagtgga ccagtaaggc
     1681 gctttctgat ttggcgcggt tatatgattt tctggtgctg gccagtaaac ctgccgccgc
     1741 cagaatggta cagtccctga tacaggcacc ggtcattctg ttaactcatc cacgtatggg
     1801 agaacagttg tttcagtttg aacccaggga ggtcagacgg atttttgctg gcgagtacga
     1861 aatccgttac gaacttaatg gccagactat ttatgtattg cgcctgtggc acacacgaga
     1921 aaacaggtag attgcgtgtg gggacaactt aacatagcct gcagatatta attcaggaac
     1981 attattatca ggccagagat tttgcattcc attaaataat cttgacgtat aggaaattga
     2041 aaaactgagc gatacccctg tccgcgcctt atgccacgtg agcttttctg cttttcccac
     2101 cactccggtg ttttctctct atgtatggtc ggtttttata ccttaatggg ccgtttttta
     2161 tacttttccc ggattgcagg cgcacctctc ctgcatcagc cagggatata cctcagcagg
     2221 cttatatccc gcgcgttttt cttcagccct gatacattca gccccgcaat ggcccggcgg
     2281 tataccatcc ggcagccaca cagcacgtat tcgaacgggt cacgactcag gaactgcgct
     2341 tcaccatttg tgcatagcac actttcgcca ctgcttccgg tttatccatc cccagagcac
     2401 ggtacacctg cggcagcttc tctccacaca cccggttggc aaggaacccg aagtacctca
     2461 ccatcttaaa aaacttctcc gggatgtgct gtttcagcct cgcgaccagc tcacgctgtg
     2521 tcagcgtttc cgtcgccgtt tctcccgttt tgtggtccag gtaacggaag ctcaggctcg
     2581 cccctccgtt gtaatgtgcc agccgggagg ctgctatcgg cggcttcttc agataacgac
     2641 ccaggtggcg cgccgtattc ctcccgtcgg ccgttttctt cgacatgtac atcaggcgcg
     2701 gaactcatcg gggcgcggga atgaaatccc attcagactc cggatagatt caagcaagcc
     2761 cacttatcgt caaaatcggt gttgcaaaaa cgggagtgac catagatccg ttttccaagc
     2821 ggaccccata atatcggctg gtaaacatca ctcgctagtg cattcttttt gttggtaatg
     2881 ataattattt acatttattc tttgatgttt tatcataatt aatgttttgt caataaatat
     2941 atggaaacat attgtttcag atgggaatat gagaaagttc atccttattt ctatgataac
     3001 gttagctagt gttagtctgg gagcctgcag acagaatgtc accataaagc aggatgcgcc
     3061 agggcagaaa gctttcctga ctgacgggca gatttacgat ttacacagtg gcaaaattat
     3121 ttctagcagc gagcttttgg cggatcttgc taccgctcga caccttatta ttggtgaaaa
     3181 acacgataat gccgagcatc atcagattga gctttggctt attcaaaatc tgctcatcca
     3241 gcgaccacag gggagtgtgt tgcttgagat gttgacgtca gagcaacagc cccgagttaa
     3301 ccaagttaaa tgttggctga aagataatcc tgtggttcga gatagccgtg ttcaggaact
     3361 acttaactgg caaaaagggt ggtcctggga gatgtatggc gacatcgtca tgcagctatt
     3421 acgtgggcct tacccgctgc tcaatgccaa cattggccgg gagcagatgt tggcactgta
     3481 caaaaaaaac gaatttccta aaggaaaaaa atccactgca cctgtggtac aagaagctct
     3541 gcgtgaaacc atcatagcta tgcatgaagg caaccttgaa agccagcaac tgacgtcaat
     3601 gctctctgtt cagcagcagc gggatcgcta catggcccgc cagcttctga gtgccccggt
     3661 accgtcgttg ttgattgcag gtggttatca cgcgtcaaaa agcatgggcg taccgttaca
     3721 catggaagat cttgccgccg gcacccatcc agtggtgttg atgcttgcag aaaaaggaat
     3781 gaacatcaca gtagatcatg ctgactacgt gtggtttgta gcgccagata caacaaaaag
     3841 gtaaaccatg gatgatgaat attctccact tcccacagtc ggtaaaatgg agcagctggt
     3901 ttatctgttc tctactgctt catggactga ttttccttgc gtttatctgg cgtttttccg
     3961 aagttcagcc cgctatgtct cctgcaccgg cgatcatgct gcaatgggca gaaattatag
     4021 aggctccttc ttctccactt tcgcttccgg tgggaatcgc tcaacaggag tccgcagtga
     4081 cagaagaaaa gcaacaaact gaggacagac aacaacgtcc tgtcactgaa gacagtgatg
     4141 caaccattga aataaccagg aagaaaaagt catccgatgg agaaaagaga aagacacgtc
     4201 cacctcgtaa gataaaggct caaaccagcg acagcaaccc cacagccgtt tccagtaatg
     4261 ctgcacctca ggctctggtc gaatcgtccc gtattgctgc gccttttaac agtgactcaa
     4321 caaagcgaga taattctgag gcatcttggg aaagcagagt aaaaggccac cttaaccgtt
     4381 ataaacgtta ccctggcgat gccagaaaac gtgcacgaac cggcacagcc gtcgtgagtt
     4441 ttaccgtaaa cacggaaggc actattgtca gtagtttctt ggaaatctct tcaggaaccc
     4501 tttctcttga ccgcgaagct attgctgttt tagaacgcgc tcaaccactt cccaaaccac
     4561 cgcctgaaat attggagggg ggattattta aagtaaaaat gccgattacc tttaagttga
     4621 aggagtaaag ggataattgg ccgttcaagt tcatttaact gaaatttaat tttcattttt
     4681 atgctgaaca ttttatcaaa cttttccact gtcgaaaaaa tcaaaaagac agtagatcat
     4741 aattaataaa caaatagtta ta
//
LOCUS       DAZFFI010000210         4669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFFI010000210 DAZFFI010000000
VERSION     DAZFFI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.212"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..1143)
                     /locus_tag="ACHJ12_004877"
                     /pseudo
     CDS             complement(41..1143)
                     /locus_tag="ACHJ12_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            1172..1447
                     /locus_tag="ACHJ12_004878"
                     /pseudo
     CDS             1172..1447
                     /locus_tag="ACHJ12_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2220..3197
                     /locus_tag="ACHJ12_004879"
     CDS             2220..3197
                     /locus_tag="ACHJ12_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4992533.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNI
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3483..4221)
                     /locus_tag="ACHJ12_004880"
                     /pseudo
     CDS             complement(3483..4221)
                     /locus_tag="ACHJ12_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4342..4572)
                     /locus_tag="ACHJ12_004881"
     CDS             complement(4342..4572)
                     /locus_tag="ACHJ12_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO4992534.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNDKTALHPLHYWLHEGSEQKKNPFPIWFYPR"
BASE COUNT         1157 a         1163 c         1088 g         1261 t
ORIGIN      
        1 aaggctgtct gtaagataga cagcctgtct tatatattat ttaaatgccc cagaacttat
       61 tcgctttttc cacaagcgtt tcatcaatca gtgcaccggt gacagccatc agtaaaccat
      121 acccgataat gcctaaagcg cttccggtaa gaatactgaa gaccagagca acaagagccg
      181 ttgcggcatt acctgctatg atggtttctg ttttaacaaa aaaggacgcc agttatctgt
      241 ccgggcagcc ttaccaaact cagtgatcca gtcagcaaaa tttgtaattt ttcctgcata
      301 tcccagtccg cgactgaacc tgttcagatt agacgatata tcagacagct tcacagactc
      361 aagggctgcg gcaatcgcag cacgatcttt tgcattaatt tttttgttaa tgtcagtccg
      421 gtacttttca tacgttttta atgcctcttc aacattacgt attttcttcc ctttagcctg
      481 cccggccatc tctctggcta actgctcagc ttttgcacca tatttttctg aaactgattt
      541 caggaactct gttgtgaaat taattgcgtc tttcgttgcc ttcagttcat cctgttttct
      601 tttctcttcc gctatcttct gattgatgcc ggcaagctgg ttacgggtat tctctttttc
      661 cttcaacagg gcattaagag cgtcatttgc atgcttttgc tcatttgttc tggcactgag
      721 gttatttctc accgaattta ccgcagattc agcagaggtg attttatttc tggcatcaag
      781 caatctttgc cgtaacttat cccattcagt aacttcagca ttataaattt tcttgtcacc
      841 ttccacatcc tctgtcacca ccggattccg gtcattcaga atattgcgtt tatagtccag
      901 accggaatga gtcagaagat aattcaggtt tgcacggtta tcgatgactg cgctggttgt
      961 gatttttatc gtatcggcaa atctcggatc accgctcaca acaatagaat gatttgttga
     1021 tgaacgcccc gggaattttt cagaggaaac tatcgttttg ccgtcaggcg tgtttttctg
     1081 ttgcgtcagc tgttgttcag ctctggttaa tgcattacgg gccgtgttaa gcgattccgt
     1141 caccgaactg agtttattcc gggcctctga aatggccctg tcattccgtg tctgctgctc
     1201 gagcagacgg gcctgttgtg catcaagaac ggcaagggct gccccgccct ttttttccag
     1261 ctctgacaac cgcgtattca gaataccttt tctgcgctcg tattcagcaa gcatatcagc
     1321 ggccttcttg tctgcgttct gcgcatcctt ctgccgtttt tcagcctgtt caattaacga
     1381 cctggcctcg cggggagata tacgaagaga atcagcatca gcaaacagca tccggctctc
     1441 tgtatgtgtc aacgccacga tgtttgaccg tcttatatca ctggcgctga caacacgaca
     1501 gagttatcag gcatggtgtc aatcagttca caatttttcc atcggagcag cctaccaccg
     1561 gtgataacct gactttgcct gtagtttgta tacacaatcc ctggcattga aacacacata
     1621 tgaagtgtac ttcggatatg accgtacgct caggaagtac tgcagattcc gtcaaaaatt
     1681 acctgtaaag acagacttca gaaaaaacag tgctcgccgc agaccggtga ccgggcgaag
     1741 cccgcgaacc ggcgaggaag cgcaatgtat ctgtagttac agtgagcagg tacgccctga
     1801 agcccttata agcggttgtg tatgctgttt cccctgggat gatttatcca catatccaga
     1861 ggttcgatcc agtaaacaga tccatgaatg atcaacaaag gatccattaa agatccccat
     1921 atcgctgtaa accttgtcat taatggtccg ggagcacgat cacataagct gtggcatgtt
     1981 actgataaac tgtaacaagt taatgataag ctgtattaag taatacatat actgcagtaa
     2041 gttaatgaca taaactatgg tcagtacgcc agactcagct gttaaataca ggctgcagat
     2101 ttttcttcag tcagttagcg gggttctgac atccgatttt ctgtttattc ttttactgtc
     2161 cacaggcagg aggctttctg gaaaacgaaa attcagacat caaaaaactg ttcggcgagg
     2221 tggataagtc gtccggtgag ctggtgacac tgacaccaaa caataacaac accgtacaac
     2281 ctgtggcgct gatgcgtctg ggcgtttttg taccgaccct taaatcactg aagaacagta
     2341 aaaaaaatat actgtcacgt actgatgcca cggaagagct gacacgtctt tccctggccc
     2401 gtgctgaggg attcgataag gttgagatca ccggcccccg cctggatatg gataacgatt
     2461 tcaagacctg ggtggggatc attcattcct ttgcccgcca taacgtgatt ggtgacaaag
     2521 ttgaactgcc ttttgttgag tttgcaaaac tgtgtggtat accttcaagc cagtcatccc
     2581 gcaggctgcg tgagcgcatc agcccttccc tgaagcgcat tgccggtacc gtgatctcgt
     2641 tttcccgtac cgatgagaag cacacccggg aatacatcac ccatctggta cagtcagcct
     2701 actacgatac tgagcgggat attgttcagt tacaggccga tccccgcctg tttgaactgt
     2761 accagtttga cagaaaagtc cttctccagc ttaaggcgat taatgctctg aagcgacggg
     2821 agtccgccca ggcactctac acctttatag agagcctgcc ccgggatccg gcaccgatat
     2881 cgctggcgcg gctgcgtgca cgcctcaatc tgaagtctcc tgtattttcc cagaaccaga
     2941 cggtcagacg ggcaatggag cagttgcgcg agattggata tcttgattac acggagatcc
     3001 agcgggggcg gacaaaattc ttctgtattc actaccggcg tccccggtta aaagcgccga
     3061 atgatgagag taaggaaaat ccgttgccac cttcacctgc ggaaaaagtc agtccggaga
     3121 tggcggagaa gcttgccctg cttgagaaac tgggcatcac gctggatgac ctggaaaaac
     3181 tcttcaaatc ccgctgaata taaactgtag tcagtgaaga gcgctccttt actgactaca
     3241 gcttatatta actggtgcag tgagtggtct gctcactgca gtttatattc ttgttccctg
     3301 cagtgctgtt tgtagctgag ctgccatctg cctgtccctt acgtaagtga tcccgtaacc
     3361 tgatgctgag gcattgctcc cttcataaaa catgacttac tcactacagc ttatatacat
     3421 gctccagctt atgttatgtc tgttctgctg accacagctt atataaggaa gcgcgtatgc
     3481 cgtcacctca ggggaggcag tgtacgcagg atctccgcag catcccgccc gtcaccggtg
     3541 aaaggcactg ccagcgtggc agccatatcc agcgcaaaca ctctggtata aacctccatc
     3601 gaacgtggat ccctgtgacc gaccagtgcc tggatgactt tccggggctg gcggtgatag
     3661 agcatgtgca tgatatagct gtgccggaag gtgtgtggtg tgaccggaat cgaaaagtga
     3721 accccgtctg cttcggcccg tctgacagcc tgcttcagcc agttgcgcat ggtttcgtcg
     3781 gtcacggccc ataatggttc acgacgccgg ggccgggtgg tgatcatcca gctttccatc
     3841 tgcctgacat agcttatatc tgtcagcgga accaggcgga cttcatcttt tggcgggcgt
     3901 ccgcgtcgcg cacgcacttt ttcggacagg atccgcacaa acggtcgcag accatccaga
     3961 tcaaatgatt ccggcgtcag catccgggct tcgccaatac gcattccggt attccagagg
     4021 gtggcaaaca gcatatggtg acgctgatcc ggcatataga agagaagggc actgacttcc
     4081 ggagccagca ggtaggccgg tgtggccccc gtggaagtgg ccattcttct cagggagagc
     4141 gctgttgcaa aatccacccg gcgcaatggg taacagggag acgcgttctg gtgaattctg
     4201 caggggaatg acattgttca tgagttattt ttcacgtagc acacgcactg tttatggata
     4261 caggtattct gacctgaacg cccggatcct tccacgacaa taaatcgcag cgcgcagatc
     4321 cttttgtttg cttatcccac attatcgcgg gtaaaaccaa attggaaaag gattcttttt
     4381 ttgctcagat ccttcatgaa gccagtaatg aagcgggtgt agggctgttt tatcgttttt
     4441 gggagaattg gggtaatacc aggacgctaa tgatctatca atggatggat gtaagtgaca
     4501 attgaaaact gaaaaatgcg ctatgtagat tctgacagat ttatgcacct gaaatacgtt
     4561 caaaatccac atagcgtaac tctgattttc cttaccagaa aaaagagggg gaataaccag
     4621 tatctccccc cccccacaca caattgcaga ataataaatt ataaaaata
//
LOCUS       DAZFFI010000211         4575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFFI010000211 DAZFFI010000000
VERSION     DAZFFI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.213"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..434)
                     /locus_tag="ACHJ12_004882"
     CDS             complement(<1..434)
                     /locus_tag="ACHJ12_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4992535.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGG"
     gene            663..1271
                     /locus_tag="ACHJ12_004883"
     CDS             663..1271
                     /locus_tag="ACHJ12_004883"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4992536.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1369..2760
                     /gene="selA"
                     /locus_tag="ACHJ12_004884"
     CDS             1369..2760
                     /gene="selA"
                     /locus_tag="ACHJ12_004884"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4992537.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2757..>4575
                     /gene="selB"
                     /locus_tag="ACHJ12_004885"
     CDS             2757..>4575
                     /gene="selB"
                     /locus_tag="ACHJ12_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4992538.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRYEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT         1010 a         1187 c         1357 g         1021 t
ORIGIN      
        1 ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg
       61 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      121 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      181 gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg gccgggcagg
      241 gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac cggatggccg
      301 gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc aatgcgcacc
      361 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg acgcgctgcc
      421 ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct tccaggcaaa
      481 accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa acaattagga
      541 aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa ccatcaacat
      601 tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac aaaggactcg
      661 gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct attctgttgt
      721 tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg gacaacggcg
      781 tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag ggcgaatgtt
      841 ggtttgattc gccgatcatc gctgaatata ttgaattaat gaatgtcgct ccggcgatgt
      901 tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcactg gcggatggca
      961 ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccagcggcg cagcagtctg
     1021 aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat
     1081 atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc gccattgcct
     1141 gtgccgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac
     1201 acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc accgaaccgc
     1261 caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc cgtggttatg
     1321 ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat gacaaccgaa
     1381 acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga tagctccttc
     1441 ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc
     1501 gacgaagcgc gagaagtgat tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg
     1561 gcgcaagaag tcgatgcccg gttgacgaaa gaagcgcaga gcgcgctgcg tccggtgatc
     1621 aacctgacgg gaaccgtgct gcataccaac cttgggcgag ctttacaggc ggaagccgcg
     1681 gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga tctggacgac
     1741 gccggacgcg gacatcgcga tcgggcgctg gcgcaactgt tgtgccgtat tacgggggcg
     1801 gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt ggcggccact
     1861 gccagcggaa aagaggtggt ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt
     1921 cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg gaccaccaac
     1981 cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa
     2041 gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga agcggaactg
     2101 gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag tggctcgctg
     2161 gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg
     2221 ggcgtcagtc tggtgagttt ctccggcgac aagttgttag gcgggccgca ggcaggaatt
     2281 attgttggta aaaaagagat gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta
     2341 cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta tttacaccct
     2401 gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc agaggtcatt
     2461 caaatccagg cacaatgttt acaggccccc cttgccgcac attacggcgc ggagtttgcg
     2521 gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg
     2581 ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct tgagtcatta
     2641 gccgcccgct ggcgtgaatt gccagtgccg gtgattggtc gtatttatga cggacgattg
     2701 tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt gttgaaatga
     2761 ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag gcgattactg
     2821 gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc gatctcggct
     2881 atgcctactg gccgcagccg gatggtcgcg tgcctggttt tatcgacgta cccggtcatg
     2941 aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg
     3001 tggcgtgcga tgacggcgtg atggcacaga cccgtgagca tctggcgatt ttgcagctga
     3061 ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac gaagcgcgtg
     3121 ttaatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt gctgaggcaa
     3181 aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc gagcatctgc
     3241 ttcagttgcc ggaacgcgag cacgccagcc aacatagttt ccgcctcgct attgaccgcg
     3301 catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc ggggaagtga
     3361 aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc
     3421 atgcgcaaaa ccagccaaca gaaaccgcca atgccgggca gcgtatcgcg cttaacatcg
     3481 cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc gatgtgccgc
     3541 cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacaccgctg acccagtggc
     3601 agccgctgca tattcaccac gccgccagcc acgtcacggg acgcgtttca ctgctggaag
     3661 ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac gatcgcttag
     3721 tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg atgcttaacc
     3781 cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct cttgcacggg
     3841 cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt aaccttgcgg
     3901 atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg caacagcctg
     3961 gctatattca ggctggttat agcttgttga atgcgccggt tgccgctcgc tggcaacgga
     4021 aaattctcga cacattagcg acttatcatg agcaacatcg ctatgaacct gggcctgggc
     4081 gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctagta ctgttgctga
     4141 ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg catctgccgg
     4201 atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca gagccgctgt
     4261 ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg
     4321 caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg atcgtcaaag
     4381 atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc gatctcgatc
     4441 aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc
     4501 tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt cgtggaaatg
     4561 atcatttatt gcgcg
//
LOCUS       DAZFFI010000212         4476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFFI010000212 DAZFFI010000000
VERSION     DAZFFI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.214"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..584)
                     /locus_tag="ACHJ12_004886"
     CDS             complement(<1..584)
                     /locus_tag="ACHJ12_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992539.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDVGFEKDLGYPCAIMPALVGYHSQYVAPYYQ
                     PIYTTSTCATGNKIYSCEHYSGAASWSNFSRYID"
     gene            complement(590..868)
                     /locus_tag="ACHJ12_004887"
     CDS             complement(590..868)
                     /locus_tag="ACHJ12_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992540.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(883..2150)
                     /locus_tag="ACHJ12_004888"
                     /pseudo
     CDS             complement(883..2150)
                     /locus_tag="ACHJ12_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            complement(2147..3772)
                     /locus_tag="ACHJ12_004889"
     CDS             complement(2147..3772)
                     /locus_tag="ACHJ12_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992541.1"
                     /translation="MPYIDITTMRGMMPGVVASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4310..>4476)
                     /locus_tag="ACHJ12_004890"
     CDS             complement(4310..>4476)
                     /locus_tag="ACHJ12_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992542.1"
                     /translation="VTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEI
                     LAYRVGWAQE"
BASE COUNT         1148 a         1230 c          935 g         1163 t
ORIGIN      
        1 ttatctatat atctggaaaa gttagaccat gaggcagcac cagaataatg ttcacaggaa
       61 tatattttat ttcctgtagc acaggtggat gtggtgtaaa tcggttgata ataaggtgct
      121 acatactggc tatgataccc caccagtgcc ggcataattg cacatggata cccaaggtcc
      181 ttttcaaatc cgacgtcaac aggtattaac ttcgcgtcaa gcagcatcat ttcgccatga
      241 taaatcatct caccgtccgg gttatacatg gcgataccat actcagaagg tggagtaacc
      301 atatttgcga aagcataaac cgtcgtaaca ccagggttcg ttcccctgac aattacatga
      361 agccttagtg catgatatcc atcaatctgt tcatgcgtgt acatgacatc ggccttcttc
      421 tctgttctga taaagaaaaa acaacttttg cctgacggga ttgatgtttt aaaaaaagac
      481 ttttcagtcg ccggtatggt gcctttgttg atcagacact gcggcgtaaa acctggactt
      541 atccatacgc tgccatccgg cttcataatg ctcaaaccat acatgacact tatccccaga
      601 atgtataaat ataagatccc ataccctgct caagattcga ccacgtcacc gtattgccat
      661 taatggttat cttcggtact ttccgatccg caaatacatt attccaggga aataaacaac
      721 atacagcctg caatgatttc ccgtcgggtt tattcgtgta cgtctttgag ccagactccg
      781 ctgtaaatct gtccaggaaa aataccggag tcagcacgcc cgtaacatta acgttatttc
      841 tgttataaat ggcaaaaccg tattccaata ctcacctcct gatcagcgta atctgcctat
      901 gcgaacagcc agtcgtccat tctgatcata aacctcaatt ttatcattgc ggatcaccag
      961 tcctacattc tgattagagt aacgaattgt cagttgccct tgtgacgtaa cactgaaaag
     1021 gcctccaata ttcaggttac cctgagaatc aacctgaaag tttccgttct gaataacggc
     1081 actccggata actggcgaag tgatacttac cccggctttt acttcatccg ccacaacctt
     1141 ccgcgacacc agtgtttcaa tcactgcgtc ataaatcatc gctttcggga tcactacctt
     1201 gccacctgat accgcaaacg gataggctgt gttatccgga ttatttgggt caaagacaaa
     1261 caactgcgac gcagaaattg caacctgact tacaggcctg ccttcactgt cttttccggc
     1321 gacaatcccg atccccgcag tgatgccatc aactcccgct ttttttgacc acatcgcaag
     1381 gaaggcctca ccgccttctt tatccagttt agtgatgcgc tgtcgacctc attaagcgat
     1441 tcactggttg acgaatccag tgtggttatg cgggtttcaa taccaccaat cgttcttgtc
     1501 gtttcttccc tgagagtccc tacaacttcg gttgtcttaa ttgcagcatc ctttacagcc
     1561 tgcccctgcg cgttttttat ttctttacgc agctcggaca caaccggcga ctttgcagcc
     1621 tcatcgcgga tctggtcaat gatggccttc acaccgatct gtgtttgtgc ctgtgtgcct
     1681 ttttcaccat tccatggacc tttcactcct gccgcgttaa caaaacgtat ccagtaaaat
     1741 cccgaccagc cagggtcaac cggatcgcca taaacctgcc cgggagttgt ggcaaccagt
     1801 accgcatccg caaggtcatc ctccgtaccc cgccagattt cagtcagtga atgtccgcga
     1861 taattaggca tatcccattc aagaagaacc gagccaaatc caccggtcgc cttaaaattc
     1921 agcggttttg tgggaaaatc aacagtcatt aaagtactgt caatctcaat acccggattc
     1981 agtgcatatg aggcaccacc cgatgttcga cgccgggcga gtttaagacc aaccagttcc
     2041 tcacgggtca caaatgcgtg acgtccgtca ccacgctgcc cggtgccaat ttccatattc
     2101 tccacaacag tggataaatc cttccccgca cgccacggtt ttctggtcat accggcatct
     2161 ccgacattga tgtactcagg gttattcgtt ccacctgccc gaatccggat accatcacct
     2221 gccagttttg cccggttgct gccggaagtc tcaccacact tcccttaaac gtacccggcg
     2281 caaaatgaat cacaggaaca tcatcagcca taatggtgat ccccacccgc tcaggcgccg
     2341 gagatttcac cctgatacag gaaaaagagg ttctttcagg taatgaaaaa atttttgaat
     2401 gccaccttat cgtggagggc agagagcccc cggcaagcac tgacattttg tctcctgtca
     2461 ccacgcgcat catatctttc gcgagatcaa cccatgcgca gtcaaacggt gtactgagat
     2521 aacggatatc catgttcacc ggactgaata caaacacatc ctgcttacca tccggtttcg
     2581 tgtaacaggc aatgtactca ccacgccagg aataagccac aatggacgcc gggttaaact
     2641 gactctgcca ctgttcaggt gaaataatct tttccgttgc cagtgctgta ttaccgttta
     2701 catcaacaga taccagaccg tttgtcccgg catagagtac gaatccctcc atcgccacca
     2761 tacttcttcg gctcaggcat gcctgcatgg aaggaattct ggagccagaa attgtggaag
     2821 gcgatacacc actgaacaga taaggctccc cctttgtcgc caccaccagt gacgttccca
     2881 gcggacagat agccacaata tcttctgccg tcgtgtgacg attcacttcc ggccatgcat
     2941 acggcagata cgcttccgaa aacatcactt cattaccggc aaacccggcg gcaataccat
     3001 tagccatcag gcaaaggcct gtcatattct ctggcggcgg caggtaatcc catgtcgcca
     3061 gagaaggtcc aaggtttttc cccggtattt tgtccgtgta actgagcacg gatgcatcca
     3121 gttcagccac aagtaaaaaa tccgcttctc ctccacctga tgcagagcga taaatccggc
     3181 ggcgtttaat actggcattc tgcaatggca ccggagacag cgtcagctgt accgcagtcc
     3241 ccggagtacg gagtgttacc tccagagacg ccggacctgg cggaccttct tcaccataat
     3301 ctgagacaaa ggtttccgta taaaaccggg tttcgtcatc attcgggtta tcgtcagaaa
     3361 catcaccgcc ctgctgaaca gtacagacag gtgctgtcgt cggcgcgggg atccccagac
     3421 gatacgatga tgtcgggtga ttcccgtccc cttttgtggc aatggtcgca tccgtcactt
     3481 taggaaaacg cccgtcagtg tagtaaatac gcccgtggga gtcctgagcg accggactgc
     3541 ggatcacatc caccacatcc gtccatgcaa accagaaatc gtcacggtaa tgaaaaatgg
     3601 ttttcggctt aatcgcgaat gttttctcag cctctgacat ctggtgttca ggcgtgatca
     3661 ctccatagcg aaaatgacag ttttctgcca gtacagcaga atgatctggc agcatagatg
     3721 caacaacgcc cggcatcatc ccgcgcatag tggttatatc gatataaggc atggttgtct
     3781 ggtatccgaa aatttgaatg gcaggtaaat cactgaattg agggtttaaa ggcggcatac
     3841 aattgcgcct gtgcagaata cagatttcca gaaatctgaa aaattactct tatcagagaa
     3901 gagcgtcaga aacacgatcg gcatcactgt ttctgcaaac ttccctgtgg cagtctttat
     3961 tcgccatata acgtagcact aaaggtcttc attaattttt ccagtatagt taatactcct
     4021 tgtaccgggg gtggtgatac ccccgctttt ttcgttatcc ctgttccctt aatcacactg
     4081 ccaggcgcca caaaacacat caattgtggc tggtatggaa acaccaaaaa ctctcacttc
     4141 cggaaatctg aaaaattatc catatcaaca ggatgcgccg gaaacatgat ttccatcaca
     4201 tccaggcctg cactttccgt gacagtattt attcgcatag taaatacttc acaagtgtat
     4261 aattgactca gttcatgggg gaggtgatgg tcccccatct ttttgtctgt tattcctgtg
     4321 cccagccaac cctgtatgcc agtatctcat ccgcactggt cagcttttcc agctccttct
     4381 tcatggtgcg ctggcgaacg tggatttcca tccctttggt gaacatcgcc tgctctgccg
     4441 cttcactcag tgctataagc tcttctgctg tcaccg
//
LOCUS       DAZFFI010000213         4423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFFI010000213 DAZFFI010000000
VERSION     DAZFFI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.215"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..1082
                     /locus_tag="ACHJ12_004891"
     CDS             291..1082
                     /locus_tag="ACHJ12_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992543.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKAKYACFIYIERTIYNHD
                     NPSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMASGAKGIPETQKYP
                     DVYCWIPLFSINSGVVITPVYKIDPRKPLTVKKPDQITVVCNYRE"
     gene            1468..1674
                     /locus_tag="ACHJ12_004892"
     CDS             1468..1674
                     /locus_tag="ACHJ12_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4992544.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1768..2370
                     /locus_tag="ACHJ12_004893"
     CDS             1768..2370
                     /locus_tag="ACHJ12_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992545.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHI
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            2843..3058
                     /locus_tag="ACHJ12_004894"
     CDS             2843..3058
                     /locus_tag="ACHJ12_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992546.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            3200..3496
                     /locus_tag="ACHJ12_004895"
                     /pseudo
     CDS             3200..3496
                     /locus_tag="ACHJ12_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1271 a         1081 c         1022 g         1049 t
ORIGIN      
        1 tttttaatgg tacatgaact gcctgtaggt gtaacgataa acaaatcacc caaaacgaaa
       61 aatactggtt atcatggttc agagaactca tacaagtggc agctcccccc ctgagcaatg
      121 cttcattttc ggtggttctg tgtgatagag ttatactgac tacagtggca gtaacactgc
      181 tttccaccac actaggataa agccagtaaa aaaatcaatt atatgaatta aaagcatttt
      241 ttatgaaatg ttatgcatat caaccagtat gattaacaga aaatttcatc atgacaacac
      301 gtataacttt caatatgacc agtgatgaga cgctgcgaat tgtggatgag tactgtcata
      361 cccacaaact ttcgcgctct aaggtaatcg acgcattact cagcgccaca gcacctgtcc
      421 ttaacgatat taactgttat tatcaacttg caggcaaact gcagtcacgc ttattaaatg
      481 gagtgtatca acgggatctt cctcacaaac gcaatgtcgt cagtgcagag aagtactgtc
      541 tggagatctg ggaaaacaaa cttttcacca aaagaatcct ggagtttgac agttcaaatg
      601 gtgttctgta cgcgctgaaa cataaaagac actatcgccg ggacaagatg attggaaggg
      661 ttgaaagccg gtgtatcaaa gacatttgcg aataccagat gcaactgtcc ggggagaagg
      721 caaaatatgc ctgttttatt tatatcgaaa gaacaatata caatcacgat aatccatccg
      781 gtgggacacc tgtaaaagcg gctgttggta atgcagtcat cctgctggca aaagacgtga
      841 tatatgacga gtactttttt gatctgcggc agtcattttt cgtgagcgtt aaagatctga
      901 tggcctctgg cgcaaaaggt attccggaga cacagaaata tccggacgtt tattgctgga
      961 tcccgctttt ctccataaat tccggcgtgg tcattacccc ggtctataaa attgatcccc
     1021 ggaaaccctt aacggtaaaa aaaccggatc agatcactgt tgtttgtaat tacagagaat
     1081 aatttcagtc ttctccgttc accgcaacag tgtttacctc tgctttatct tccgttcagt
     1141 aaatacaaac caccgctctg gcaggtacag ggcgtggttt atctccgata ctcccctgcg
     1201 cagtaataat cctcactgaa aatactgatc ccgtcacggc aaaacgccat cccatattcc
     1261 ggcaacacaa aatcatcttc cgtttatacc ggcttcactg acaaaacaga caacctgtat
     1321 tgcctgtaca caacagtttc tctcctggca ggaatattcc ccctactgat tttttacgtt
     1381 tctccccggt tgttatgccg gtgaaggtgg tgcgttgttt tcatcatcaa cgccggttga
     1441 gattaacaac atccggagga gatttttatg accaccccag tttcgctgat ggatgaccag
     1501 atggtcgaca tggcgtttat cactcaactg accggcctga ccgataagtg gttttacaag
     1561 ctcatcaagg atggggcctt tcctgcgccc atcaaaatgg ggcgcagctc ccgctggctg
     1621 aaaagtgaag tggaagcctg gctgcaggcg cgtattgcac agtcccgtcc gtaattttta
     1681 accactaacc cttcgcccac agcagacgca atctccggcc tgccggcggc actctgctgc
     1741 ctgttatccc ccgtgaggaa tatgaaaatg aaacaacagt atcagacccg ttacgagctg
     1801 ctccatgaaa actaccagaa atggctgacc ggcttcaccc ggcacgccgt atcatggggc
     1861 gtgtgtcatc cgaatatcta ctatttccat aatctgacgc ccgggtgggt gtcattcaat
     1921 ggcgaaaaac cggagattgc catcgttccc cagagtctgc accggctgat ttatggtccg
     1981 gacaaacgga gctccccgtc cctggatgat gatctggtgg tgaatttatg caccagcgaa
     2041 cacctgctgg ttcatcatcc gatgctggaa gggatcctgc tgtctgaatg tgagcgcctg
     2101 aagcaacatt ctctggcaaa caaactgatc agcctcttcc gtcagtttgg cggcacggag
     2161 ttacgtctca aactggtctg gctctgctgg cttgacttaa tgaccggaaa cagccttgac
     2221 gactggacga aaaacctgaa acacatatca gaaaaagatc tggagcaatg gatcattgca
     2281 cgtcagggtc agagtgaacc actgacaaat ctgatggatc agtacgtgct tatggcatac
     2341 cgtacatcgg ttgatgccgc tcacagttga tgtctgagaa ccacagggct ggcagcagaa
     2401 aaccagccct gtggtaaaat acagtaatag cggcgtacat caggcacgcc gctcactcgt
     2461 ccacagatca tatacgtcat tatactgcgc gataacggag ggcttcagca cccggtcatt
     2521 tctgagcggg tataaatacc attgatgatg cgaagcagcc acgggcagaa acgttcttca
     2581 caaccaggtg cctccatctt cactgcaggc tgaatgtgcc ggcttgttat ccaccagaaa
     2641 tggtatactc cggtacatgc ataccccgga gactataaac gcactgaatc atagcctccc
     2701 acctcagcag gaggcagtca gctttcaggc ggaaaaacgt tgcagttaaa gatcgtgaga
     2761 atcgctgttt cgttgcatag caacccgaac agttgtagtg aaacatttta cctgcatcag
     2821 cacatggtca ggcgccgtct gtatggcgtg gaatactgat gtttcggctg atggcaagcc
     2881 tcattctctg tggaaacagg ggcgcgcatt agcttctcaa ccagagagtg tcacaaagac
     2941 aaatattgct gaaaatcgca ttcatcgttc aggcgtgaat gcccggagac aaaagccgct
     3001 gtccgcacag cccccctctg acctgaacaa aaagcataca cacaacaggc tcacctgacc
     3061 ccgaaaaacc ataacttcag caataccagt gacaccagca agttacccca gtgccacatc
     3121 ttcagaccgc gatcaatctg aaatgtgact atcggggcaa tatccctgct ttgtggtaca
     3181 cgataaccag aaaacatccc ctgaaccgcc agccagaaag actggccaca aaagggggaa
     3241 gctgaaatcg tttatggatt acatcgacat atgcctggca gagaatgtcc ggaatgacga
     3301 ggttatcttt gcggagatta aggcaatggt atacgggccg acgttccatg ccgcgttact
     3361 acattcagcc cggacgtaaa atgcgcccgt caaaaagaac ggttcgcttc gaaactcagc
     3421 caggatacca cctctccgcc acatccggga agaagttgat atggtggttg ccggacaaca
     3481 gcgctcctgg attgactgct gcatcactca accacgctga atatcaaagg agagcgttac
     3541 cggttaaaag agaaacgtaa agctgtggtg atgacccaaa acgcaacgcc tgtcagtgag
     3601 gatgaaatgg tgaaaagcgg acagcatcag taacggaagt atcttagcgg gtacgaaaat
     3661 ggcaggatcg tgacattgac agaatatctc ctgatcatcc tgaatgactg cagttgctgt
     3721 ggaaaagtaa gctgtctcca cccacggcac cacttaacag gcattccgta actaccccca
     3781 gacttactat gacgcattat cggtgcactg aaaacaggag agtgcgctta aaaccgcaga
     3841 ccgcattgcg ggtgttactg catgacagtc tggccgtatt gtgagatgtc ggcaggaacc
     3901 gatgtccgcg cagggggaac ttcctgcagt acctgacgct tcaaatggta ttaaccgtag
     3961 ggcgttgttg gttccggatc gcggaaatgg gagcacaggt ctgctacggg gtgtacctct
     4021 gacagtaaat ccggctcgct catctgactg cctctctccc ggaaaagacc aaaaatgaga
     4081 tcgtctcctc aacagcagag aagaggtctt gacagacagg ctacattgca tacgatttat
     4141 gaagaaaaag acagcaaata tggaggttat agcggagaac accctacgaa attcctatga
     4201 aaaatgattg aaaaaaatat caaattcgat tcgtttttat atgctttttg ggaacgtggc
     4261 gatgacgaac tgttgcatgt gcagaggaga atgcacccgg aagcaccaga atcatccaga
     4321 aaggcagaac gattacgcat caaaccagga gacagcaata tcaaggtaat gtactgattt
     4381 aaaatgaatt tacagcaata cgtgaaaaca ttttttactt aaa
//
LOCUS       DAZFFI010000214         4396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFFI010000214 DAZFFI010000000
VERSION     DAZFFI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.216"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..747
                     /locus_tag="ACHJ12_004896"
     CDS             <1..747
                     /locus_tag="ACHJ12_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO4992547.1"
                     /translation="NLFGGKGYFSYPKPTSLIKTLFGSVRTEDKDIYLDFFAGSGTTA
                     HGILELNIEDGRKRNFICVQLDEETDKKKQAYKEGYKSIFNITKDRIIKAGKKLKKDN
                     PNYNGDLGFKIFETVHDFRAKDESELTLSNLSFFDDAVLTPEQYDTLLTTWCVYDGSL
                     LTTPIEDIDLNGYKAHLCDGRLYLIAPNFTSEALKALLQKLDADKDFAPNKVVFYGSN
                     FESAKQMELNEALKSYANKKSIDLDLVVRN"
     gene            750..3662
                     /locus_tag="ACHJ12_004897"
     CDS             750..3662
                     /locus_tag="ACHJ12_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO4992548.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDAIKEHFRDDYERELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHILVINSGMINSKSLTDTYDVGLLDNQFDTPFSALSA
                     IKPFIIIDEPHKFPTGKKTWEHIQKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKLVKSDGKEATFELNENNNKKIFKLAKGESLSKTHSAI
                     HDLTLDALNKNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKTEKDEFYRNYLEK
                     TVKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKNRDFTLNYYVDFTEKDFV
                     DSLVKEVNDSSFKETVPGKFTQELKDKILSQYPELSSRTLMNEIFDAEIIDDNENFKD
                     SDAYSRLKAKYPAAFPTGVKPTKIKKATDGKKRTKMRIGKFSELKELWDLINQKVVIE
                     YKINSENEFLSIFKSFMIEETDRFTKSGVHTRIDRIYIHNDTAMSKSIVSDDDNFAKL
                     NTMSYREFLDNLSQTIFAKHDTLHKVFCDIKDTINITDYLNIQTIRKIKSGFSKYLLN
                     NSFNKFSLGYNVISGSIHPTKFTNADGNHLHEVLSGDLGVLQDNTKAPLDSYLFEEVF
                     YDSELERLNITDGEIHSVAVFTKIPKNSIKIPVAGGYTYSPDFAYVVKTTKGDYLNFI
                     IETKNVEGKESLRKEELDKIKHAQKLFEQVSKSVKVTFITQFSGDRIHDLIKNVIQ"
     gene            3746..>4396
                     /locus_tag="ACHJ12_004898"
     CDS             3746..>4396
                     /locus_tag="ACHJ12_004898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992549.1"
                     /translation="MTTCSELKERARFDGMHDASLEVAWQLLMTKSVSTITTEIISGA
                     DMSNFSVIIRERYKHAIAKSLLNHLSPDVVSDIIGLSHDEFNQQYKGNLTRQQHLRDI
                     AVSLTDKGFTESSACNIVNLDLMLLPEENWLLYKENYNTGYQDALYKSVWRMHTYEYP
                     TATISCLTKLNNENILNIITGIEVILLAAKKPACKDRTDKIAEMLKIPFSVVKEFID"
BASE COUNT         1579 a          738 c          790 g         1289 t
ORIGIN      
        1 aatctttttg gtgggaaagg atatttctcc tacccgaagc caacaagttt aataaaaaca
       61 ctatttggtt cagtaagaac cgaagataaa gacatttatc ttgacttttt tgctggctct
      121 ggaactacag ctcatggtat tctggagtta aatattgaag acggcagaaa aagaaacttc
      181 atctgtgttc aattagatga agaaacagat aaaaagaaac aagcctataa agaagggtat
      241 aaatccatct tcaatataac aaaagacaga attattaagg caggaaaaaa gctgaaaaaa
      301 gataatccta attataatgg agatttggga tttaaaatat ttgaaacagt tcatgatttt
      361 cgagcgaaag atgagtctga acttacgctt tctaatctta gtttctttga cgatgctgta
      421 ttgacaccag agcagtatga cactctattg acgacatggt gcgtgtatga tggcagcttg
      481 ctaacaacac caattgaaga tattgacctt aatggctaca aagcacactt gtgcgatggt
      541 cggctatatc taattgcgcc taatttcaca agtgaggctt tgaaagcttt acttcaaaag
      601 ctggatgcag acaaagactt tgcccctaac aaagttgtat tttatggcag caacttcgaa
      661 agtgcgaaac agatggaact caatgaagca ctaaaaagtt atgctaataa aaaatctatt
      721 gatttagatt tggtggtaag gaactaatca tgtctaaagg gttcacattc gaaaaaaatt
      781 tacctcatca aaaggctggc gttgattcgg taatgaatgt ctttgtatcc gccatacctc
      841 atcagacgga tcatgttgca attcgcctgt tagctaatcc agaactgaat ctatctgaac
      901 aacaatacta caacaacata aaaaatgttc aggagttcaa tggtattgag cactcaaaag
      961 ataattacga tgctaaaagc aacgtaattg atgtttctat ggagacaggt acaggtaaaa
     1021 cttatactta caccaaaaca atttttgacc tgaacaaatc atttggtatt aacaaattta
     1081 tcatcattgt tcctacccta tcgatcaaag ccggaaccgt taacttttta aaaagcgatg
     1141 ctataaaaga acactttcga gatgattacg agcgtgaact aaagacttac gttgttgaaa
     1201 gtcagaaaag ttcgggtaaa aatacaaaat cgtatatgcc tcaagctatc catgattttg
     1261 ttgaagcaag taatttcaat aagaaatata tacacattct tgtgatcaac tcagggatga
     1321 ttaactctaa gtctttaact gacacttatg atgttggctt gcttgataat cagtttgata
     1381 ccccattttc tgcacttagt gcgataaaac cattcattat cattgatgag cctcataaat
     1441 ttcctactgg taaaaagaca tgggaacata ttcaaaaatt taatgcccaa tatatcatcc
     1501 gctatggcgc aacctttagt gaaggctata agaacttagt ttatcgtctt acagccgtag
     1561 atgccttcaa tgaagatctt gttaaaggta ttgatgcata cattgaggat atagttggtg
     1621 atggtaatgc caacctcaag ttggtcaaat ctgacggaaa agaagctaca tttgaactga
     1681 acgaaaataa taataaaaaa atttttaagt tagccaaagg tgaatcacta tctaagacgc
     1741 atagtgcgat ccatgattta actcttgatg ctttaaataa gaatacagca gtgttgagta
     1801 atggtattga gctaaaaatt ggtagttcga taaaccccta ttcttacgat cagacacttg
     1861 ctgataacat gatgcgtaaa gctgtcaaag aacactttaa gttagaaaaa gagtatctga
     1921 cacaaaggcc acgcataaaa cctcttactc ttttcttcat tgatgacatt gaaggctatc
     1981 gtgatggtaa tgatattgca ggaagcctga aaactaagtt cgaagaatac gttttggcag
     2041 aagcaaatga actgttaaaa acagagaaag atgagtttta cagaaactac cttgaaaaaa
     2101 ctgttaagga tatatcttct gttcatggtg gttatttttc aaaagataat agtgataaag
     2161 atgataaaat tgagcaggaa atcaacgaaa tccttcatga taaagagctt ctattgtctt
     2221 tagaaaatcc tcgccgtttc attttctcta aatggacgtt gcgtgaggga tgggataacc
     2281 caaatgtctt tcagatttgt aagctccgtt cgagcggtag caccacatct aaacttcagg
     2341 aggtagggcg tggtttacgt cttccagtga atgaatacat gtgccgtgtg aaaaaccgcg
     2401 attttactct caactattat gttgatttta cggaaaaaga ttttgttgat tctcttgtaa
     2461 aagaggttaa tgatagttct ttcaaagaaa ctgttccagg taagtttact caagaactta
     2521 aggataaaat cttatctcag tatcctgagc tttcatccag aacactcatg aatgagatct
     2581 ttgatgctga aattattgat gataatgaaa acttcaaaga ttccgatgcc tatagccgtt
     2641 taaaagcaaa atatccggct gcattcccta ccggggtgaa accaacaaaa ataaaaaaag
     2701 ccacggatgg aaaaaagcgt actaaaatgc gtattggtaa gttcagtgag ttgaaagaac
     2761 tatgggattt gatcaatcaa aaagtagtaa ttgagtataa aataaatagt gaaaatgaat
     2821 tcttatccat tttcaaatca ttcatgattg aagaaacaga tagatttact aaatcaggtg
     2881 ttcatactcg cattgataga atttatattc ataatgatac ggcgatgtcg aaaagtatcg
     2941 tcagtgacga tgataacttt gctaaactaa acacaatgag ctatcgggaa tttcttgata
     3001 acttatcaca aactattttt gctaaacacg ataccttaca taaggttttt tgtgatataa
     3061 aagacactat caatatcacc gactacctga acattcagac aatcagaaag attaaatctg
     3121 ggtttagcaa gtatttgttg aacaattcat ttaacaaatt cagccttgga tataatgtaa
     3181 tttcaggctc aattcatcct acaaaattca ctaatgcaga cggtaatcac ctgcatgagg
     3241 tattatcagg tgatcttgga gtattacagg ataatacaaa agcccccctg gactcttatc
     3301 tttttgaaga agttttttat gattcagagc ttgaaagact caatataaca gacggagaga
     3361 tccactctgt tgctgttttt acaaagatac cgaaaaactc aattaaaatt ccggtggcag
     3421 gtggatacac atattctcct gactttgctt atgttgtaaa aacaacaaag ggtgactacc
     3481 ttaactttat tattgaaaca aaaaatgtcg aaggtaaaga aagtttacgt aaagaagaac
     3541 tggataaaat aaagcatgca caaaaacttt tcgaacaggt aagtaaatct gtaaaagtaa
     3601 cttttataac tcaattctca ggtgacagaa tccatgatct gataaaaaat gtgattcagt
     3661 aaacacatgc atgataacaa agtggaggtg gtttcacccc cactttataa ttatacacac
     3721 atcacaaaat tcgcaggtga gttcaatgac aacatgttct gaactgaagg aaagagcccg
     3781 ttttgatggt atgcatgatg cttctcttga agtggcatgg caattactta tgacgaaaag
     3841 tgtatccact atcactactg agataatatc cggtgcagac atgtccaatt tttcagtgat
     3901 tatcagggaa cgatataaac atgcaatagc aaaatctctt ctcaatcatt tatctcccga
     3961 tgttgtttct gacatcatcg gtttatctca tgatgagttc aatcaacaat ataaaggcaa
     4021 tttaaccaga caacaacacc tgagagatat tgctgtaagt ttaacagata aaggattcac
     4081 agaatcatct gcatgtaata ttgtcaacct ggacctaatg ttacttcctg aagaaaactg
     4141 gttactttat aaagaaaatt acaatacagg atatcaggat gcactttata aatccgtatg
     4201 gagaatgcac acatacgaat atccaacagc aaccatcagt tgtttgacaa aattaaataa
     4261 tgaaaatata ttaaatatta ttacaggtat agaagtaata ctcctcgcag caaaaaaacc
     4321 tgcatgtaaa gacagaacag ataaaattgc tgaaatgctg aaaataccat tctctgtcgt
     4381 caaagaattt attgat
//
LOCUS       DAZFFI010000215         4317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFFI010000215 DAZFFI010000000
VERSION     DAZFFI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.217"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..720
                     /locus_tag="ACHJ12_004899"
     CDS             <1..720
                     /locus_tag="ACHJ12_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4992550.1"
                     /translation="HVQVGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGA
                     GLYASALFDSGAHIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTE
                     DAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFE
                     KSAFGKYNVDNAVNASFRYSF"
     gene            complement(1288..3960)
                     /gene="rcsD"
                     /locus_tag="ACHJ12_004900"
     CDS             complement(1288..3960)
                     /gene="rcsD"
                     /locus_tag="ACHJ12_004900"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4992551.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGNALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1094 a         1002 c         1074 g         1147 t
ORIGIN      
        1 cacgttcagg tgggtgcgga taaaaaacat gaactggacg gtctggacct cttcaccggg
       61 gtgaccatga catataccta cagcaacgcc ggcagcgaca ccttcagtgg taaaacgaag
      121 tcagtgggtg caggtctgta cgcttcagcc ctgtttgatt ccggggccca tatcgacctg
      181 attggtaagt atgttcacca tgataatgag tatacggcgt cctttgccgg actcggcaca
      241 aaagactaca gctctcattc ctggtatgcc ggtgctgaag tgggttaccg ctatcatgtc
      301 acagaggatg cctggattga gccacaggcg gaactggtct atggtgctgt atccggtaaa
      361 cagttcagct ggaaggacca ggggatgagc ctgtccatga aggacaagga ctacaatccg
      421 ctgattggcc gtaccggtgt ggatgtgggt aaatcgttct ccggtaagga ctggaaagtg
      481 acggcacgtg ctggtcttgg ctatcagttt gacctgctgg cgaacggaga aacggtactg
      541 cgtgatgcat ccggagagaa gcgtattaag ggcgagaaag acggccgtat gctgatgtct
      601 gtgggtctga acgcagaggt ccgtgacaac atccgttttg gtctggagtt tgagaaatca
      661 gcctttggta agtacaacgt tgataatgct gtcaacgcca gcttccgtta ttcattctga
      721 cggttgttac tgaaggggat tatggcctga cggctccctg taaataaaga caggcacaga
      781 agattgtgag ttactcagtg ttccaccaaa aagtaaggtc gcaactctgt gcctgcttcg
      841 taagtgtgca gtgagggcca tatggtcata actgaagcat ccgacgacgt ggtgtctacc
      901 agagtgtcag atatgcagga aaatatgagt tccgacagaa atgcagtaaa aaaaggagtc
      961 tcgcactcct ctccagaaaa ctccagggct gtaagttgta ttaaaaagct gatttttctt
     1021 tagcgataca gacctcaata agtataacac ccttcagtta ctgtggttat gcacaggtgt
     1081 tatttttaac agtgtggata aacagtgtcg attattcaga ttggttatgg ggcatgtatg
     1141 ggacattttt aatcggcgaa atacgtcgaa gttcgtcgat atggtaaacg aatcatctat
     1201 ccaacccttg aaaaacggcg ctcctggacg atcttcgtcg atttttaaaa tgcaacaagt
     1261 acgttgttca tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc
     1321 gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc
     1381 acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc
     1441 cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc tcttaacatc
     1501 atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc
     1561 atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg
     1621 taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt gaccaggccc
     1681 aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga
     1741 cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat
     1801 acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt
     1861 aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt caacttccgg
     1921 gtcagctgcg agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt
     1981 gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga atgccagcgg
     2041 gtccgccttg ccatagcgat cgttttgggt ctggttgata aacgggaagt gcaaattatc
     2101 catttcatga atacttacgc cttctcccgt gtccagaatg cggaacgtca ggcggtcttc
     2161 ggaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc
     2221 atattgcatc agtagcagca aaatacgtcg taaggcatcg cgatcgccgc ggcgcatatc
     2281 gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa
     2341 cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg tctcactttt
     2401 ccagctatca tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc gcaccagcac
     2461 atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg cgctctccgc
     2521 cagggactgt gcgggttctt tcagcgcatt gccaatgttt ttcataaagg tcatccgccc
     2581 ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc
     2641 gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcgacctggc tgcggaacat
     2701 gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc
     2761 catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact
     2821 tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac
     2881 tatctcttca ttaattgccc gtaaaatgcg caattcgtta ttgaccgccg tgctgggtag
     2941 actttctgta ctgcggctgg agaaatggcg gaatgtggta tagccaaata acgccagcgc
     3001 cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcaataa
     3061 ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggcgat
     3121 ttcaatcttc gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattatc
     3181 attgtttccc gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg
     3241 tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg
     3301 gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt
     3361 agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc aacatttctg cacgacgtga
     3421 atcaacaatg tcactgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag
     3481 ggttgagatc agcaccagac tgttatcctg accattcagg taatacatcg accacggtac
     3541 attttctgcg ccccacaatg tgtccagata ggtggacatc cgctgagtca tctcaagcgt
     3601 tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgcggtt tctccagata
     3661 gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt
     3721 cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg
     3781 gatagcgtgg gtaatgtcga caatctggta acttttatct ttcagccatg cgttaacggc
     3841 gctttgtacc attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg
     3901 ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat
     3961 tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt
     4021 gaaattccag actcttacct gattgatgat tctctattct cccgtgatat ttatcatcct
     4081 gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct acaaaaagcc
     4141 caacgaaaat aattgcaaat aaacaaagat tgctggaaat tatgctgatg ttaattattt
     4201 gtgaaatagt taacaagcgt tatagttttt ctgtggtagc aaagaataat gaaaagtgtg
     4261 taaagaaggg taaaaaaaac cgaatgcgag gcatccggtt gaaatagggg taaacag
//
LOCUS       DAZFFI010000216         4265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFFI010000216 DAZFFI010000000
VERSION     DAZFFI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.218"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHJ12_004901"
     CDS             complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHJ12_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4992552.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1321..1653
                     /gene="hdeA"
                     /locus_tag="ACHJ12_004902"
     CDS             1321..1653
                     /gene="hdeA"
                     /locus_tag="ACHJ12_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4992553.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1769..2095
                     /gene="hdeB"
                     /locus_tag="ACHJ12_004903"
     CDS             1769..2095
                     /gene="hdeB"
                     /locus_tag="ACHJ12_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4992554.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2159..2806
                     /locus_tag="ACHJ12_004904"
     CDS             2159..2806
                     /locus_tag="ACHJ12_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4992555.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLLNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2848..3378)
                     /locus_tag="ACHJ12_004905"
     CDS             complement(2848..3378)
                     /locus_tag="ACHJ12_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4992556.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(3534..4100)
                     /gene="slp"
                     /locus_tag="ACHJ12_004906"
     CDS             complement(3534..4100)
                     /gene="slp"
                     /locus_tag="ACHJ12_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4992557.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1300 a          871 c          900 g         1194 t
ORIGIN      
        1 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
       61 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca
      121 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaat gccagttgca
      181 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      241 tgttcacttt cccactgcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
      301 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
      361 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacgc
      421 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
      481 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
      541 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
      601 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      661 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
      721 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
      781 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      841 aattatgact gcggttgctg aataacccga caataagcgc aatgcccgag cagattaata
      901 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
      961 acccgacgat aaacaacagc acggcaataa actggattgc tctgcgatgt tttttaagca
     1021 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     1081 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     1141 cgatttaata aaaatttcct aattacagta tctgatgcat ctgtaactca ttgtattgaa
     1201 ataaaaatat ctgattttga tattttctat caacatgaca tatacagaaa accaggttat
     1261 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     1321 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtaagcaat
     1381 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg ttaactcctg gacctgtgaa
     1441 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     1501 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     1561 ccagctatcg ttcaggcttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     1621 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
     1681 cttgcatatt gaacaggccg gaatatcttc tttaaaagca gctattcctc ctgttcatat
     1741 ataatctcta tattgaatgg gttacaaaat gaatatttca tctctccgta aagcgtttat
     1801 ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca caatctgcgc tggcagccaa
     1861 tgaatccgct aaagatatga cctgccagga atttattgat ctgaatccaa aagcaatgac
     1921 cccggttgca tggtggatgc tgcatgaaga aacagtatat aaaggtggcg ataccgttac
     1981 tttaaatgaa accgatctca ctcaaattcc taaagtgatc gaatactgta agaaaaaccc
     2041 gcagaaaaat ttgtatacct tcaaaaatca agcatctaat gacttgccga attaatgagg
     2101 tacaagtaaa aaggagtagc aagttgagcc atcttgctgc tcctttttgc atttttatat
     2161 gacagcagaa tttattatac gtcttatact cgcggcaatt gcctgtggcg ctattggcat
     2221 ggaaaggcaa atgcgcggca aaggagcagg gttacgcaca catgtattaa ttggcatggg
     2281 aagcgccctg tttatgattg tttcgaaata tggttttgct gacgtgctgt ctttagatca
     2341 cgtcggactc gaccccagcc gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg
     2401 tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt ctgacgacgg cagcggatat
     2461 ctgggtgacc gccgccatag gtatggttat tggcagcggt atgtacgaac tgggtattta
     2521 tggttcagtg atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat
     2581 gaacaaaaat taccatttac agctgacatt actgaacggt aatacggtat caatgctcga
     2641 ctggtttaag cagcaaaaaa taaaaacgga tttagtttcg ttgcaggaaa atgaagatca
     2701 tgaagtggtc gccattgata tccagcttca tgccacaacg tcgatagaag acctgttgcg
     2761 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg
     2821 tagccagact caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac
     2881 cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat ggcagtagaa
     2941 cgtttttagc gatattttca gtttctccag aatatcttcc gttgtcattt gattcgcaat
     3001 gcagctcaga acctgatgtt gcttgcgcga aagtgacaca gccgcttcag gcacagagca
     3061 gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt
     3121 caccggcact ttccccaggc agcgtttaat attaaagggg gagaaaataa tcacatggac
     3181 aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt
     3241 atcaataatg acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat
     3301 atgaacgaca ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct
     3361 ggtaattata agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc
     3421 caaagataat taacaggaca gggcttataa taataatagt tagcttactg tatattaatt
     3481 ttatgtaata catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg
     3541 accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt
     3601 tccggccaga atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct
     3661 ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata cgggacttta
     3721 ttgataaagc ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga
     3781 tagttcactg gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct
     3841 tcaatatcag gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc
     3901 gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat
     3961 aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct
     4021 ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt
     4081 gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat
     4141 atttctttat agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt
     4201 ctgatgttta tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta
     4261 tccat
//
LOCUS       DAZFFI010000217         4212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFFI010000217 DAZFFI010000000
VERSION     DAZFFI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.219"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHJ12_004907"
     CDS             complement(<1..209)
                     /locus_tag="ACHJ12_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4992558.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHJ12_004908"
     tRNA            complement(324..399)
                     /locus_tag="ACHJ12_004908"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHJ12_004909"
     tRNA            complement(406..480)
                     /locus_tag="ACHJ12_004909"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHJ12_004910"
     tRNA            complement(597..681)
                     /locus_tag="ACHJ12_004910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHJ12_004911"
     tRNA            complement(690..765)
                     /locus_tag="ACHJ12_004911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJ12_004912"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJ12_004912"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4992559.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJ12_004913"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJ12_004913"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4992560.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJ12_004914"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJ12_004914"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4992561.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHYVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1096 a         1043 c          940 g         1133 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acattattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgct gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 gactgatggc ttccttcacc ctaatcaggt actgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cacctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctatcttatt tttccgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatccc caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgc tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtatgagca tggccgccaa cgtattacga tcgagattga ttcccgcttc ctgcagcgtg
     2401 atccaccctt gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggca
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtta gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atcagcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataacgggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc gggacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcaccag agtgaaattc gccattcgct aacagcgcga ttaatttcag cggaacggta
     3061 ttatccttca tgaaatcgtc tccactgcgc taacttcacc tgatgcacca ataaagcgaa
     3121 cttcgggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca taatgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gaggactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatctgcct ggggataatt tggtgctgtt ggaaattgtg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcacct
     3661 gcttgcctgt cgccagttca acacagtcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtaccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tttgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 cggggggatg gcttgtagat atgatgacag gaagagtttg tagaaacaca aaaaggccca
     4201 gtcttccgac tg
//
LOCUS       DAZFFI010000218         4178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFFI010000218 DAZFFI010000000
VERSION     DAZFFI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.220"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..552)
                     /locus_tag="ACHJ12_004915"
     CDS             complement(<1..552)
                     /locus_tag="ACHJ12_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4992562.1"
                     /translation="MAFKHYDVVRAASPSDLADTLAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRTTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGI"
     gene            complement(1117..1193)
                     /locus_tag="ACHJ12_004916"
     tRNA            complement(1117..1193)
                     /locus_tag="ACHJ12_004916"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Arg,seq:tct)
     gene            complement(1207..1283)
                     /locus_tag="ACHJ12_004917"
     tRNA            complement(1207..1283)
                     /locus_tag="ACHJ12_004917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1247..1249),aa:Arg,seq:tcg)
     gene            complement(1293..1368)
                     /locus_tag="ACHJ12_004918"
     tRNA            complement(1293..1368)
                     /locus_tag="ACHJ12_004918"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1333..1335),aa:Ile,seq:cat)
     gene            complement(1409..2467)
                     /locus_tag="ACHJ12_004919"
     CDS             complement(1409..2467)
                     /locus_tag="ACHJ12_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4992563.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFCDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     WRFEQTIREVQDLVSQNG"
     gene            complement(2619..2816)
                     /locus_tag="ACHJ12_004920"
     CDS             complement(2619..2816)
                     /locus_tag="ACHJ12_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4992564.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(3034..3594)
                     /locus_tag="ACHJ12_004921"
     CDS             complement(3034..3594)
                     /locus_tag="ACHJ12_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4992565.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLDKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKLKMAERLNADHPEWTLVTCRRRIDQWL
                     GISEFMLHAPMRMAFVTEKKMLQTDQ"
     gene            complement(3603..3962)
                     /locus_tag="ACHJ12_004922"
     CDS             complement(3603..3962)
                     /locus_tag="ACHJ12_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4992566.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDELTHAGLLMDDEQFDEINIVRGQ
                     FVPGGRLGVKIYKIESE"
     gene            complement(3975..>4178)
                     /locus_tag="ACHJ12_004923"
     CDS             complement(3975..>4178)
                     /locus_tag="ACHJ12_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4992567.1"
                     /translation="ADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEK
                     KYGSQIELIFRFLDHAFATGVLG"
BASE COUNT          996 a         1187 c          942 g         1053 t
ORIGIN      
        1 aatacccgca ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatgtc
      121 ctgcacatca tgcagacaat ggtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 acccggtgtc actgtagtgc ggcgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc agcggaaggc cttcaccata tgccatgctg tttgactgcc ccgccagaac
      301 cacaacaaag taatactccg ggtcgctggt ggcgctgatt actgtgcctt ctccatccga
      361 cggcttcacc acaacaggtg tgctcacatc accttctgcg acaatcgcct gaataagtgc
      421 tgcgccatca tccgtatacg aagaaaacgg cccgccgtat ggttgccatc cttcacgaat
      481 tttttgagca agtgtatcag caaggtctga cggcgacgcc gccctgacca cgtcatagtg
      541 tttaaatgcc atgaatcctc ccggccggga taatgttgtg agtcagataa ggggcgggct
      601 gaagtccgga agttacagga caatgacaga aggaagacta cagcccgcaa tacgaaaaag
      661 gccgcgcagt tgcgcagagt gattactgtc gggtattatt cgccagctga aaaattgatt
      721 acttcacgtt ttgttgttta ttccttgccg cccgcgtctc ctggcgcggg ctttttttat
      781 ccataaaaaa gcccctccgg agaggggctg acgctgcgta tctgtatcat gatgtacatg
      841 gtgccgggtg cctcccggtg agttcggccc ggtgccaccg aacccgcgtc gataatgaat
      901 cacaagtaag gatttttcac cagtcgcccc tccgcacagg gggattcacc atgcgatgcg
      961 ctttttaaca aattcgccgt cagccagaca atcatcaact tacgaaattg tgaggcattt
     1021 aaaaatttca atggttaact gatactctgc taaccacccg ttgttttctt tttcagcgac
     1081 aaaaaggccc accgaagcga gacagaaaaa tcaatgtggc gcgtcgtact ggattcgaac
     1141 cagtgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgcatgatgc
     1201 agataatgga ccgccatcgg ggacttgaac cccgcgcagc cagcttcgaa ggctggcgct
     1261 ctatcccgat gagctaatgg cggtatgtga tatggtagcc cttgctggat ttgaaccagc
     1321 gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg acgcaggata
     1381 ataacggtac gtaactaatc ctgcaatatc atccgttctg actgactaaa tcctgtactt
     1441 ccctgatcgt ctgctcaaaa cgccaggtct ccagctcaac tccagttgca cgacgcccca
     1501 gcgccatcgc ggctttgact gtcgaacccg accccatgaa gaaatctgca accagatcac
     1561 ccggacgact gctcgcgctg attatctgct gcagcatttc tgccggtttt tcgcacggat
     1621 gtttcccggg atagtactgc accggtttat gcgtccacac atcggtgtac ggcacctgca
     1681 ccgtcacgcc aaaataccgc cgcagatgct tatattcact ctgcagttcc gtatactgcc
     1741 ggttcagttc actgtatgtg ctgaccagtt ggtgatgtgg cttttccagt tctccgcgct
     1801 gatgcttctc ttctgccacc cgggcaaaca gcgcctgtaa tttcagataa tcgctttcgt
     1861 tcggtagctg ccactgactg gcactgaacc agtgcgacac catgtttttc tttcctgtgg
     1921 catccactat ctgttttgcc gttatcccca gggcagcacg cgcatcacaa aagtaagaaa
     1981 tcagcggggc catcacatgc tgtttcagtg ccctgccctt cgcctcatac ccggcatctt
     2041 tcggacgata cggcccctga taatgttccg cgaacagaat gcgctctgtg gcggggaaat
     2101 acgcccgcag gctttccttg ttgcacccgt tccagcgtcc ggacggcttc gcccagataa
     2161 tatggttcag cacactgaag cgttcacgca tcatgatttc gatatcagat gccaggcgat
     2221 gaccacagaa caggtaaaga cttccggcag gtttcagcac ccgccagaac tgcgcaagac
     2281 actggtccag ccacttcagg taatcatcgt cgcccttcca ctggttatcc cagccctcag
     2341 gcttcacttt aaagtacggc gggtccgtga ctatcaggtc aacagaattt tcgggtaacg
     2401 accggataaa ttccaggcag tcggcgttga ttaactcaca actggatatt tttacagtat
     2461 taaacatgga tcattaagcc tgtctctgat aggctcattc tgcttttgcg caaagcagat
     2521 gggcctgagg tttgcttgtg atcctgacgc atgagcagat ggctggtgag tgcccctaac
     2581 acccaccagc cgcccattta ccacaaataa aaaagccttc aggactgaag gcgtctgtaa
     2641 caaccgaact gatagtctgc cagacccgcc ataacaagct gggtcagtat taactgacag
     2701 cgttcgcgtg aaaggtaagt attctgcgca atctccccga ctgtcgccgg ttcggtgacg
     2761 cttaattcat taaacaccac tctggcggtt tctgtcatat cctgctgttt cagcatgtct
     2821 ttttcccttt tccggttaac gtgacacacc aataactctt gtcgaaaaag ccagcaagct
     2881 gaaagaacgg tattaataac caccagcgaa tttattgcgc tgctgtatat cgcggacaca
     2941 aaaaaaccac cttccggtgg cttccttgtg cgaaaaaaac ttgcgtttcg cctcgcgata
     3001 cagccttgcg aagcttatac ggattgaagc agtttattga tcagtttgca acattttttt
     3061 ctctgtaaca aaagccatac gcatgggggc atgtaacata aattcagata tccccaacca
     3121 ttgatcaatt cggcgtctgc acgtaaccaa cgtccactca ggatgatctg catttaacct
     3181 ttcggccatt ttcagtttgc tcattcctcg tccttcgtac ctttgccgga gaatattgat
     3241 tagcccggga tattccccaa gcacctcact gataacgcga tcaataatca acgcctctgt
     3301 gtctgtacaa tgtgacaacc agctcttctg cttccctctg gtcatatccc gaaaaaatgc
     3361 ctcaagttcc ggtttatcca gcccggattt cttcattctg cgtaaaacct cattaactgc
     3421 tgttttcgtc agctttttcg aaaccagtaa ccggttaaac atatttccgg atttaccccc
     3481 accgatatac gaccaccgcc cccacatccg taatttcccc tggatccaga ctgcttccag
     3541 cgtgttcagg cgtaaatgtt cgccgctttt gcctgtaatt tccggatata tcatatttac
     3601 gctcactcac tctcaatttt gtaaatcttc acacccaacc gaccaccagg aacaaactga
     3661 ccacgtacaa tattgatttc atcaaactgc tcatcgtcca ttaacaaccc cgcatgcgtc
     3721 agctcatcca gcggtgcttt cagaatattg tccaggtcac gacgacgctt atccggtggc
     3781 tctgcaataa tttttattgc cagccgtccg gacaggctta atttcagtcg ctgctggcga
     3841 acaataagcg ccactgcccg gcgataacgc tccccggctt ttgagacaaa atatgtgctg
     3901 ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gtaaaaccaa atctatgagc
     3961 atcagtcacc tcttttaccc gagcacgcca gtcgcaaagg cgtgatcaag aaaacgaaaa
     4021 attaactcaa tctgagagcc gtactttttc tcaaactcca gcgggtctgc atgaagttcg
     4081 ttgtggtgct cccggcacaa cggtagcgtg aaaatatcgt gggcctttgt ccccattccc
     4141 ccctgaccat gaccaatcag gtgatgcgga tcgtcagc
//
LOCUS       DAZFFI010000219         3974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFFI010000219 DAZFFI010000000
VERSION     DAZFFI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.221"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..91
                     /locus_tag="ACHJ12_004924"
     CDS             <1..91
                     /locus_tag="ACHJ12_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992568.1"
                     /translation="FKHYPLMKVISAQQAISALKYMVDGEIYF"
     gene            556..1107
                     /gene="ybcL"
                     /locus_tag="ACHJ12_004925"
     CDS             556..1107
                     /gene="ybcL"
                     /locus_tag="ACHJ12_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO4992569.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATITYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1117..1914
                     /gene="ybcM"
                     /locus_tag="ACHJ12_004926"
     CDS             1117..1914
                     /gene="ybcM"
                     /locus_tag="ACHJ12_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4992570.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHTLTVDNNQIILFPCGYEFYIENYPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIR
                     NKTSFFLPQNPELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSK
                     QSLISRCYNLLLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            2031..2132
                     /locus_tag="ACHJ12_004927"
     CDS             2031..2132
                     /locus_tag="ACHJ12_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992571.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            2129..2584
                     /gene="ybcN"
                     /locus_tag="ACHJ12_004928"
     CDS             2129..2584
                     /gene="ybcN"
                     /locus_tag="ACHJ12_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO4992572.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWVVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            2584..2754
                     /gene="ninE"
                     /locus_tag="ACHJ12_004929"
     CDS             2584..2754
                     /gene="ninE"
                     /locus_tag="ACHJ12_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4992573.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            2747..3037
                     /gene="ybcO"
                     /locus_tag="ACHJ12_004930"
     CDS             2747..3037
                     /gene="ybcO"
                     /locus_tag="ACHJ12_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4992574.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQIIWLKEGVIKA"
     gene            3034..3396
                     /gene="rusA"
                     /locus_tag="ACHJ12_004931"
     CDS             3034..3396
                     /gene="rusA"
                     /locus_tag="ACHJ12_004931"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4992575.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            3393..3533
                     /gene="ylcG"
                     /locus_tag="ACHJ12_004932"
     CDS             3393..3533
                     /gene="ylcG"
                     /locus_tag="ACHJ12_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4992576.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            3619..>3974
                     /gene="quuD"
                     /locus_tag="ACHJ12_004933"
     CDS             3619..>3974
                     /gene="quuD"
                     /locus_tag="ACHJ12_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO4992577.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMGRLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLE"
BASE COUNT         1150 a          779 c          933 g         1112 t
ORIGIN      
        1 ttttaaacac tatcctttaa tgaaagtaat atccgcccag caggcgataa gtgctctcaa
       61 atatatggtt gatggtgaga tttatttcta aataatgatc tcggatttta agttatgcta
      121 tggtgataaa gtgcaagaca gaattaatta tctttgacga aacttaatgg gtaattactt
      181 tgtttgctcc cacaagcgag ttttgtacgg ctgtattggg ggagtaaatg agctatacaa
      241 tcttaatcat ttgttaggtg agaactcttg gtcgcagatt caaatactga aaatacgtga
      301 caaattatta tgagcaaaat ggtgtctgtc acgtattttg aatggtaggt taaaaaataa
      361 caccgacttt cgtaggtatt actaataata aagcagagtt tttagatagt atcaatgtgc
      421 tttgtgtata ttgtggcaaa taattgggtt gggggtacaa ttgtgattgc ttttgcataa
      481 acattgcgcc tttatgcata atgagataaa ggaatatcaa ataaaataac gataggtcat
      541 aacaaagagg tttttatgaa aacacttatc gtttcaactg tattggcatt cataacattt
      601 tctgcgcagg ctgcagcatt tcaggtcact agtaatgaaa taaaaacagg agagcaactt
      661 acaacgtctc atgtcttttc tggatttggg tgtgaaggtg gtaatacatc gccctcatta
      721 acctggtctg gtgttcctga aggtaccaaa agctttgccg taactgtata tgatccagat
      781 gcacctacag gcagtggttg gtggcattgg actgttgtta atattccagc aacaataaca
      841 tatttgcccg ttgatgcagg gagacgtgat ggaacaaaac tgccgactgg tgctgttcaa
      901 ggccgaaatg attttggcta tgctgggttt ggtggcgcat gtcctcctaa aggagataaa
      961 ccacatcatt accagtttaa agtatgggct ctaaaaactg aaaagattcc tgtagattct
     1021 aactccagcg gagcgttagt tggttatatg cttaatgcta ataaaatcgc aaccgctgag
     1081 ataacaccag tttatgagat aaagtagggt gagagtatgc tggcaagagg taagactaac
     1141 ttaaagatcg aagaaatacg gatgcataaa catcatgaga ttcatagggt taagcctctt
     1201 atgccagctt tgtgtcgtat ccgtcaggga aagaaagtta tcaattggga gacgcatact
     1261 ttaactgttg ataataatca aataatatta tttccttgtg gttatgaatt ttatattgag
     1321 aattatcctg aagcagggct ttatcttgca gaaatgcttt acttacccat tgatttaatt
     1381 gagagtttcc aaaaacttta tacggtaact gatcaaatac gtaacaaaac aagtttcttt
     1441 ttacctcaga atcctgagtt aatatattgt tgggagcaac taaaaacatc tgtttcccga
     1501 ggcttctcaa ctaaaattca ggagcactta gcaatgggcg ttctactttc gttaggagtg
     1561 aatcatgtta atcatttact tttatcatat agtaaacaat cattgataag tcgttgttat
     1621 aacctgctgc tatccgaacc cggcacaaaa tggacagcaa acaaggttgc tcgatatctc
     1681 tacatttctg tttctacatt acatcgccgt ctagcaagcg agggggtaag tttccaaagt
     1741 atactggacg atgtgaggtt aaataatgcg ttgtctgcta tacaaacgac ggtaaaacct
     1801 ataagcgaga ttgccagaga aaatggttat aagtgtcctt ctcgttttac tgaaagattt
     1861 cataatcgtt ttaatataac accaagagag ataagaaaag cttccagaga gtaaaagtgt
     1921 tttaagaagg agcaattcta tcgattttga ttttgggaaa tcaacacggc ataattatgt
     1981 caccggagcc tgaacaactc cggtgacttc tgcgctaaac ggggacgttt atgcgcacat
     2041 acaatccaaa ctctcttctc ccttcacaga tgcagaaatg cacctgcaat tctttgcatc
     2101 tagcgtttga cctctgcgga ggtgaagcgt gaacctctca caagacggca tcaaattaca
     2161 tcgcggcaac ttcaccgcta tcggtcggca gatccagcct tatctggagg agggcaaatg
     2221 ctttcgcatg gtgcttaaac cgtggcgtga gaaacgcagt ctttcccaga atgcactcag
     2281 ccacatgtgg tacagcgaaa tcagtgaata cctcatcagc aggggtaaaa cgttcgccac
     2341 tccagcttgg gtaaaagatg ctctcaaaca cacatatctc ggttatgaaa ccaaagacct
     2401 ggttgatgtc gtaaccggtg atatcaccac tatccagtcg ttacgccata cctccgatct
     2461 tgataccgga gagatgtatg tcttcctgtg taaggttgaa gcctgggtgg tgaatattgg
     2521 ctgccacctg actattccgc agagctgcga gttccagctg ctccgcgaca agcaggaggc
     2581 gtaatggcta caccgcttat tcgtgtcatg aacggacaca tctacagagt atcaaatcgt
     2641 cgtaagcgta agcctgagct gaagccatcc gaaataccaa cactgctcgg atataccgct
     2701 agcctggttg ataaaaaatg gttgcgactg gcagcaagga ggaatcatgg ctgatttgag
     2761 aaaagcagcg cgtggtcggg aatgccaggt aagaatccct ggcgtatgta atggcaattc
     2821 tgaaacgtct gtactggcac atatccggct ggctggattg tgcggtaccg gtatcaaacc
     2881 gccagacctg attgccacca ttgcatgttc tgcctgccac gacgaaatcg accgccgcac
     2941 acattttgtc gatgctgcat atgcaaaaga atgcgcgctg gaaggtatgg cgagaacaca
     3001 gattatctgg ctgaaagagg gggttattaa ggcgtgaata cctacagtat cacattaccc
     3061 tggcctccga gcaataatcg ctattaccgc cataatcgcg ggcgcacgca cgtcagtgca
     3121 gaggggcagg cataccgcga taacgtcgcc cgaatcatta aaaacgcaat gctggatatc
     3181 ggcctggcta tgcctgtgaa aatccgcatt gagtgccaca tgccggatcg ccgtcgccgt
     3241 gacctggata atctgcaaaa agccgctttt gacgcactca ctaaagcagg tttctggctg
     3301 gatgatgctc aggtcgttga ttaccgcgtt gtgaagatgc ctgttaccaa aggtgggagg
     3361 ctggaactga ccatcaccga aatggggaat gaatgatgtt tgagtttaat atggcagaac
     3421 ttcttcgcca ccgctggggg cgtctgcgct tatatcgttt ccccggttct gttttgaccg
     3481 attaccgaat actgaagaat tacgccaaaa ccctgacagg agcaggagta tgaagtcaga
     3541 gataacaatc aactaatact gttttgttga tttttgcttg taattggcgt tctggtctga
     3601 tttttgtgga gtaagttgat gcgtgatatt cagatggttc ttgagcgttg gggagcgtgg
     3661 gcggctaata atcatgaaga tgtgacctgg tcgtccattg ccgccggttt taagggatta
     3721 attccttcaa aagtaaaatc tcgcccgcaa tgttgtgacg atgacgcgat gatcatttgc
     3781 gggtgcatgg gccgtctgaa aaagaacaac agcgatttgc acgatttatt agtagattat
     3841 tatgtagtcg gtatgacatt catgtcactg gcaggtaagc attgctgctc tgatggttat
     3901 atcgggaaaa ggttacagaa ggctgagggc ataattgaag ggatgttaat ggcattagat
     3961 atccggttag agat
//
LOCUS       DAZFFI010000220         3841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFFI010000220 DAZFFI010000000
VERSION     DAZFFI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.222"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..1421
                     /locus_tag="ACHJ12_004934"
     CDS             84..1421
                     /locus_tag="ACHJ12_004934"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO4992578.1"
                     /translation="MIGLIISLILLIYLIYRGFPVLIVSPLMAMLAVFMGSDIPVFYS
                     WTGPFMDSVVSFISAYFPIFLVGAVFGTLMNASGAALTIAHFISDKMGEKRAIIAVII
                     ASFLLTYGGISVFVVVFAVLPIARTIFIKSNYPQRLLPSAICAGCLPAYVAPGSAQFI
                     NAIPIPVFNTTIYSGLVIGIVGTAVWLLFAVWYMSAKIKNAKIKGEGYGTEVSNDCNL
                     ETGKVTLAFIPILIVIIGNAFLTYFFQIDEVVDYYRDFGGVQGTWAILISIAIAIVVT
                     ICIYWNTFKSDIKKTLTLGATESLLPIFNTSTQVGYGGVIKVLPVFTLIQTTIFSIPG
                     VALFPAIIGTAILSGIVGSTSGGVGLVMVTFGQDLLELSQAQNISPGLMHRIIVFSAS
                     TLDTLPHSGFIITLLGVCGLSHKQSYKELFIVTCVFPAFAVLSMLVYAFLFLI"
     gene            1434..2285
                     /locus_tag="ACHJ12_004935"
     CDS             1434..2285
                     /locus_tag="ACHJ12_004935"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFO4992579.1"
                     /translation="MSIYSRKQCTLEDAVNMIHDNDCVILGGFIGAVVPECIEKGIED
                     RFLKTGKPRNLKIIFAAGQGDAGTKAVNHLSHEGLVTSAIGGHWGLIPKLQKLANEGK
                     ITGYNLPQGVICHLFRDCAAGKLGTLTHVGLGTFVDPRVEGGKINDKTQEDIVSVTEI
                     NGVENLFYKKIDANIAILRGTTSDQSGNITMEDECLILENLAAAQLVHNNGGKVIVQV
                     KQIVRDGTLDPLQVRIPGIYVDAVVVSEDEYHMQTFAEYMNFSYVGKGVKEVKDKETR
                     PLDIKKL"
     gene            2300..2998
                     /locus_tag="ACHJ12_004936"
     CDS             2300..2998
                     /locus_tag="ACHJ12_004936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992580.1"
                     /translation="MELKKNAVVNYGIGAPEVIAEVTDEEGITSELIATVEPGAIGGT
                     PAGGLSFGASSFPQAIITQDQMFDFYDGGGLDQAFLGLAETDEKGDLNVSKFGTKIAG
                     CGGFINITQNAKKVYFCGSFTAGKNKIYVSNGKLHIKEDGSVKKFIKKVQQITFSSDT
                     ARKNNKEVFYITERAVFRLSQKSIELIEIAPGVDLQKNIIECMEFVPEISPSLKIMDE
                     RIFLEQPMGLELKD"
     gene            3001..3417
                     /locus_tag="ACHJ12_004937"
     CDS             3001..3417
                     /locus_tag="ACHJ12_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFO4992581.1"
                     /translation="MVYTIDKLHLGQKASFEKTITEADVLIFSGISGDINSVHINEEA
                     AKKSIFKKRVAHGVLVSSLTSAVLGMKLPGEGTIYLRQENNFRKPVFIGDTVKATCEV
                     VDINKEKNIVKLSTITTNQNDEVVIEGIAVVMAPKS"
     gene            3443..>3841
                     /locus_tag="ACHJ12_004938"
     CDS             3443..>3841
                     /locus_tag="ACHJ12_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HFO4992582.1"
                     /translation="MIIKPVIKGVVSKSAHPEGCRHAIMEQIKYCQSHLQQINNGPKR
                     VLILGASSGFGLAARISLTFGGAKCDSIGVSFEKGPSEKSIGTAGWYNNIFFKEFAEA
                     EGRIAHNVIGDAFSDDVKNKVLNLINKDFDG"
BASE COUNT         1191 a          568 c          809 g         1273 t
ORIGIN      
        1 aattgtgcag ttaactttga aataagtatc tcttgcttgt caggagatac ttaaataata
       61 atattaatat tgagagttat aatatgatag gcttgataat ttcacttatt ttactaatat
      121 atctcatata tcgtggtttt ccagttctga tagtatcccc tttgatggca atgctggctg
      181 tctttatggg aagtgatatt cctgtttttt attcatggac cggtccattt atggactcgg
      241 ttgtgagttt tatatccgca tacttcccta tatttcttgt tggtgctgtt tttggaacat
      301 taatgaatgc aagtggtgca gcattaacta ttgcccattt tatatcagat aaaatgggtg
      361 agaagagggc tattattgca gttattattg catcattttt attaacttat ggaggaatat
      421 cagtttttgt tgtggttttt gcagttttac ctattgcgag aactatattt ataaaaagta
      481 attaccctca aagattatta cccagtgcta tctgtgctgg ttgcttacca gcatatgtag
      541 ctcctggttc tgcccagttt ataaatgcaa taccaattcc tgtgttcaat acaaccatat
      601 attcaggttt agtaattggt atcgttggca ctgctgtatg gttattgttc gctgtatggt
      661 atatgtctgc aaaaattaaa aatgctaaga taaaaggaga aggatatggt acagaagtaa
      721 gtaatgactg taatcttgag actggaaaag ttactttggc atttatacct atactgatag
      781 ttattatagg aaatgcattt ttaacatatt ttttccagat tgacgaagtg gttgattatt
      841 acagagattt tggaggtgtt cagggaacat gggcaatttt aatttccata gcaattgcta
      901 ttgtcgtcac tatttgtatt tactggaata cttttaaaag tgatataaag aaaacactta
      961 cattaggtgc tacagagtct cttttaccaa tatttaatac atctacccaa gttggttatg
     1021 gtggggtaat taaagtcctt cctgtgttta cccttattca aacaacaatt ttttctattc
     1081 ctggagtagc attatttcct gcaattatag gtacagcaat tctttcggga atagtgggct
     1141 caacatcagg aggggtggga ttagttatgg taacgtttgg tcaagactta ttagaactat
     1201 ctcaagctca gaatatttcg ccaggtttaa tgcatcgtat tattgttttt agtgcatcaa
     1261 cgctggatac gcttcctcat tctggcttta ttatcacatt gctaggagtt tgtggcttat
     1321 ctcataagca aagttataaa gagcttttta ttgttacttg tgtgtttccc gcatttgcgg
     1381 ttctctccat gttggtatat gcattcttat ttttaattta aaagaggttt aatatgtcta
     1441 tttattcaag aaaacaatgt acactagaag atgccgtaaa tatgattcat gataatgatt
     1501 gtgttattct tggcggattt attggtgcag ttgttcctga gtgtatagaa aaaggcatag
     1561 aagatcgttt tttgaaaacg ggaaaaccaa gaaatctcaa gattattttt gctgctggtc
     1621 aaggggatgc aggaacaaaa gctgtcaatc atttatctca tgaggggctt gtcactagtg
     1681 ccattggcgg gcattgggga ctaataccta aattgcagaa gttagctaat gagggaaaaa
     1741 ttacgggtta taatcttcct caaggggtta tttgccattt attccgagat tgtgctgctg
     1801 ggaagttagg gacattaact catgttggtt taggtacatt tgttgatccc cgcgtagagg
     1861 gcgggaaaat taatgataaa acacaagagg atattgtgtc cgtcacagaa attaatggtg
     1921 tggaaaacct attttataag aagattgatg ctaacatagc aatcctcaga ggtactacat
     1981 ctgatcagag tgggaatata acaatggagg atgaatgttt gattcttgaa aacctagctg
     2041 cagcccagtt agtccataat aatggaggga aagttattgt tcaggtaaaa caaatagtac
     2101 gtgacggtac attagatcct ttacaagtca gaattccagg tatttatgtt gatgctgttg
     2161 ttgtttctga agatgaatat catatgcaaa cttttgcgga gtatatgaac ttcagctatg
     2221 tcgggaaagg tgtaaaagag gtaaaagaca aagaaacacg ccctcttgat ataaaaaaat
     2281 tatagctcga cgagccgcaa tggaactgaa aaaaaatgca gttgtaaatt atggcatagg
     2341 ggcacctgaa gttatagctg aagtaacaga tgaagagggg attacctccg aacttatcgc
     2401 aactgttgaa ccaggtgcaa ttggaggaac gcctgctgga ggtctgtcat ttggagcttc
     2461 ttcgttccct caggcgataa ttactcagga tcagatgttt gatttctatg acggtggtgg
     2521 gcttgatcaa gcgtttcttg gattagctga aacagatgaa aaaggagatt taaatgtttc
     2581 taaatttgga acaaaaattg cagggtgcgg aggatttatt aacataactc aaaatgctaa
     2641 aaaagtatat ttctgcggtt ctttcactgc tggaaaaaat aagatttatg tcagcaatgg
     2701 gaaattgcat ataaaagagg atgggagtgt aaaaaaattc attaagaagg ttcaacaaat
     2761 cactttctcg tcagatactg caaggaaaaa taataaagaa gtgttttata ttacagagcg
     2821 tgctgttttt agactttctc aaaaatccat tgagttgatt gaaatagcgc ccggagtcga
     2881 tttgcaaaaa aatattattg aatgcatgga gtttgttcca gaaatttctc cgtctttgaa
     2941 aattatggat gagcgtattt ttttggaaca accgatggga ttagaactaa aggattaatc
     3001 atggtataca ctattgataa actacactta ggtcagaagg cgagctttga aaaaacaata
     3061 acagaggcag atgttttaat tttttctggt atttctggcg atattaattc tgttcatatt
     3121 aatgaggaag ctgcaaagaa gagtattttc aagaaacgag tagctcatgg tgttcttgta
     3181 tcatctctaa catcagcagt attaggtatg aaactcccag gtgaaggcac aatatatctt
     3241 cgtcaggaaa acaattttcg caaacctgtt tttattggtg acactgttaa agcaacttgc
     3301 gaggtagttg atattaataa agaaaaaaat attgtaaagc tttcaacaat cacaacaaat
     3361 caaaatgatg aggttgtaat cgaaggcatt gcagtagtca tggctccaaa atcttaattt
     3421 atttaactat caaggacctt atatgattat aaaacctgta attaaaggtg ttgtctcgaa
     3481 aagtgctcat ccagagggat gtcgtcatgc gataatggaa cagattaagt attgccagtc
     3541 ccatcttcag caaattaata atggcccgaa acgagttctg attctcggtg catcttcagg
     3601 atttggtctt gctgcaagaa tttctctcac ttttggcggc gcaaagtgcg attctatcgg
     3661 agtttcgttt gaaaaaggtc cctctgagaa atctatagga actgcaggat ggtataataa
     3721 tatattcttt aaggaatttg ctgaagctga aggcagaatt gctcataatg ttattggcga
     3781 tgctttctct gatgatgtaa aaaataaagt tcttaactta ataaataagg attttgatgg
     3841 c
//
LOCUS       DAZFFI010000221         3822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFFI010000221 DAZFFI010000000
VERSION     DAZFFI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.223"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            92..319
                     /locus_tag="ACHJ12_004939"
     CDS             92..319
                     /locus_tag="ACHJ12_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992583.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(396..638)
                     /gene="yfdY"
                     /locus_tag="ACHJ12_004940"
     CDS             complement(396..638)
                     /gene="yfdY"
                     /locus_tag="ACHJ12_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4992584.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            991..1911
                     /gene="lpxP"
                     /locus_tag="ACHJ12_004941"
     CDS             991..1911
                     /gene="lpxP"
                     /locus_tag="ACHJ12_004941"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4992585.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2267..2338
                     /gene="ypdK"
                     /locus_tag="ACHJ12_004942"
     CDS             2267..2338
                     /gene="ypdK"
                     /locus_tag="ACHJ12_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4992586.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2403..3641)
                     /gene="alaC"
                     /locus_tag="ACHJ12_004943"
     CDS             complement(2403..3641)
                     /gene="alaC"
                     /locus_tag="ACHJ12_004943"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4992587.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          998 a          965 c          889 g          970 t
ORIGIN      
        1 aacatcgtag gttctcagtg aaccattttc aggataactt tctatgaaag taaacttaat
       61 acttttcagc ttatttttat tggtctctat tatggcatgc aatgtttttg cattttccat
      121 ttctggtggt gtaagtgaga ggagctataa agagactgaa aaaacatcag cgatgacaac
      181 cacacactct acaaaacttc agccatcaca ggcgattttg tttaagatga gagaagatgc
      241 gccaccatta aacctcacag aagaaatcac gcctacttat ccgacaaagg cgaattatct
      301 tattcatcct gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt ctgatgctga
      361 tgtgaacacc tgcccaaatg agccatcctg acccactatc gcctgccata ccccaccata
      421 aagcgaatca tcaagccaca agccggggca agaaacataa atatccccgt cactagttca
      481 ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat caccatgccg
      541 aagaaagaag agaccgccga taccacgttc agtacctgac cgatgtagcc gcttacgcag
      601 acaatacaca gggcgagaaa catccataaa taaatcatag ctcaacctcc tgccgcgcta
      661 aaaaacctgt aacttcagtc acttttatag taagacaatc cttaacctct ggcaattttt
      721 gctaagcatc aatgaaaaca gcatgttaaa tgcaagactg ttgtgtacgg aaaaatattt
      781 actttgcacg attaataatc agtagctgaa agcagtcagc gagtgataaa gtacagatac
      841 cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt acactcttta caggaaccat
      901 tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt cgacatggaa tcttctgcga
      961 tacaacaatt cgtatctaca gaaggtaact atgtttccac aatgcaaatt ttcccgcgag
     1021 tttctacatc ctcgctactg gctcacatgg tttgggcttg gtgtactctg gctttgggta
     1081 cagcttcctt atcctgttct ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca
     1141 ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt cccgcagcat
     1201 tctgcggaag aacgcgagaa gatgattgcc gaaaactttc gttcactcgg catggcgctg
     1261 gtagaaaccg gcatggcatg gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt
     1321 gaagggttgg ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc
     1381 gtccatttta tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca accaatgatg
     1441 gctacctatc gtccacataa taatcagctg atggaatggg tgcagacccg tgggcgcatg
     1501 cgctctaaca aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag
     1561 aaaggtgaag cggtatggtt tgctcccgat caggattatg gtcgtaaagg cagctccttc
     1621 gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt
     1681 ctctctggcg cagccatgtt gaccgtaacg atggtaagaa aagcggatta cagcggatat
     1741 cgtttgttca tcaccccaga gatggaaggc tacccgacag atgaaaatca agccgctgcc
     1801 tatatgaaca agattatcga gaaagagatc atgcgcgcac cggagcagta cctctggatc
     1861 caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta acaaactgct
     1921 cttactcttt ccctcctcaa atcggccata ataacctcat ggtttttatg gcttttttct
     1981 taaggttttt tccttcgctg aatatctaat tattaccgtc agaaattaaa ctgtcgccga
     2041 tatgagacac ttgtaagtta ctaaaagtaa ataagaaaat gtctcttgtc tcgtttcagt
     2101 tttaggcgta cattagcgcc gtctcgagca gtttagctca gaattctggg gtgatgccca
     2161 cggtaaaata cagggctttt aaggtcgctt tgcccggtgt caactcactg tatcaggtgt
     2221 aatgaagtca ttcaggcgta acagtaatta cgcggagaga tgtaaagtga aatatttctt
     2281 tatgggcatt tcttttatgg tcatcgtttg ggccggtact tttgccctga tgatctaaaa
     2341 gcaaactgtg caatgaaaaa caggagccat caggctcctg tttgcatttc tgacgacggt
     2401 gtttattccg cgttttcgtg aatatgtttg ctgctggcgg gtagtaaacc gtcggcacgg
     2461 aacatcgctt taatcccacg aatcgcctga cgaatacggt cgcggttttc aatcagtgca
     2521 aagcgaacat gcgtatcacc atagtcgcca aagccaatcc ccggcgagac acaaaccttc
     2581 gcttcgttaa ggagcttctt ggcaaattcc agcgatccca tggccgcata tggttccggg
     2641 attttcgccc agacatacat cgaagccttc ggcatttcaa ccatccagcc cgcttcatgc
     2701 agccctttaa ccaatacatc acggcggcgt ttgtactgtt cggcaatgtc gcgcacgcac
     2761 tgttgatcgc cctccagcgc cgcaatcgcc gccacctgca acggcgtaaa ggtgccgtaa
     2821 tcgtgatagc ttttaatacg agccagggcg ctgaccagcg ttttgttgcc aaccataaag
     2881 ccgatacgcc agcccgccat gttgtagctt ttcgacagcg taaagaactc gactgccaca
     2941 tcgcgtgcac ccggtacctg catgattgac ggcgctttcc agccatcgta gacgatatcg
     3001 gcataggcca ggtcgtggac caccagcaca tcgtagcgtt tcgccagcgc cacaaccttt
     3061 tcgaagaact ccagctccac gcattgcgcg gttggattcg acgggaagcc gaggatcatc
     3121 atcttcggtt tcggataact ttcacgaatg gcgcgctcca gttcgttgaa gaaatcgaca
     3181 ccttccacca gcggcactga gcgtacctgc gccccggcaa tcaccgcgcc ataaatatgg
     3241 atcgggtaac ttggattcgg caccagcacc gtgtcaccat gatccagcgt cgccagcatc
     3301 agatgcgcca ggccctcttt cgaaccaata gtgacgatgg cttctgattc cgggtcgatt
     3361 tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc gacgtaaccg cggaatgccg
     3421 cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca cagtacataa tttttcgacg
     3481 atatgcggcg gagtcgcacc gtccgggtta cccatgctga aatcgataat atcttcgccg
     3541 cgccgacgcg cagccatttt cagttcagcg gtgatgttaa aaacgtaggg cgggagacga
     3601 tcaatgcgcg taaagcgacg ttcagggcga gtgtcagcca taatttcctc agattaacgt
     3661 tagcgcccgg accgtccgag cgacgctgcc acaatggtgg ctgttttgaa aatagcctga
     3721 ttaatttctg tctgtccagc gttttagaac atttttatca atggctttaa atgaggaact
     3781 ccacttcaaa aacaggaaat ttttctacgc taacaagaaa at
//
LOCUS       DAZFFI010000222         3745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFFI010000222 DAZFFI010000000
VERSION     DAZFFI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.224"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            95..2374
                     /gene="ydeP"
                     /locus_tag="ACHJ12_004944"
     CDS             95..2374
                     /gene="ydeP"
                     /locus_tag="ACHJ12_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO4992588.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGKRYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2622..2819
                     /gene="safA"
                     /locus_tag="ACHJ12_004945"
     CDS             2622..2819
                     /gene="safA"
                     /locus_tag="ACHJ12_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4992589.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            2893..3654
                     /gene="ydeO"
                     /locus_tag="ACHJ12_004946"
     CDS             2893..3654
                     /gene="ydeO"
                     /locus_tag="ACHJ12_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4992590.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT         1038 a          833 c          865 g         1009 t
ORIGIN      
        1 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
       61 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      121 gggtgctgca ggtgggtggg gtgctgtcaa atccgtagcg aatgcagtac gtaagcagat
      181 ggatatacgc caggatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      241 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      301 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      361 gaatacggtt caatcattac tttcctgggg agatcacgag cttgaggctg cgggacgtct
      421 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      481 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      541 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      601 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga ccagtgtggg
      661 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
      721 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
      781 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
      841 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
      901 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
      961 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1021 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1081 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1141 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1201 cactcagcac gaacatggta cccagaacgt acagcaactg gtcaatttgc tgttgatgaa
     1261 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1321 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1381 taagcgctat ggcttcaccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1441 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaatt ttgcgctggc
     1501 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1561 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1621 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     1681 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     1741 atcagagtgt gctgtggtcg cgggaatcgc ccaggcaaca ctaccccaga gcgtggtagc
     1801 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     1861 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     1921 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     1981 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2041 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2101 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2161 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2221 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2281 atcgaatcac atgctaactc ttgataacca cgatccatta agtggcattc ctggttataa
     2341 aagtattccg gttgaattag aaccatcaaa ttaattcgcc tcctcatttc ttctgctgtc
     2401 atccgcacag cagaagaatt tctcattgac tattatcgcg caatttgctc acattgatta
     2461 aattaaacca cacactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2521 gtactgaagc accagtccta tttcctcttt tctccagcct gttatattaa gcatactgat
     2581 taacgatttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     2641 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt gtggttgtct
     2701 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     2761 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     2821 aataaacagc aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt
     2881 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc cttcaacgct
     2941 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3001 gttcgtacgc attttgaacc ttatgagcgg cattttaaag agatcggatt tactgaaaat
     3061 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3121 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3181 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3241 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3301 ggaaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3361 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aacttaagca agagcaaacg
     3421 acattctcac agattctttt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3481 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3541 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3601 caaaggcaca cgggtatgaa tacgggcaac acgatgaatg ctttagctat ttgattattt
     3661 gctaacgagt agtcaaccac gcaccctacg taaaaattaa aaatggggca gtcattccct
     3721 gccccgcgtt gtttttaggc gatat
//
LOCUS       DAZFFI010000223         3656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFFI010000223 DAZFFI010000000
VERSION     DAZFFI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.225"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            141..1613
                     /locus_tag="ACHJ12_004947"
     CDS             141..1613
                     /locus_tag="ACHJ12_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4992591.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            1617..2447
                     /locus_tag="ACHJ12_004948"
     CDS             1617..2447
                     /locus_tag="ACHJ12_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4992592.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            2493..3203
                     /locus_tag="ACHJ12_004949"
     CDS             2493..3203
                     /locus_tag="ACHJ12_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFO4992593.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDVNKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            3216..>3656
                     /locus_tag="ACHJ12_004950"
     CDS             3216..>3656
                     /locus_tag="ACHJ12_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFO4992594.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGV"
BASE COUNT         1014 a          595 c          870 g         1177 t
ORIGIN      
        1 aaatttgtaa tgtaatgctg tttccggcat atttcagaag tgactctggt cttattggtg
       61 caactctttg ggaccgggat tgtattactt aaagtaatac tccagtgtat tataaatccg
      121 ctattattgt gaggtttaat atggactcct gtattcagaa aaagcaggtt tggtataagc
      181 atctttcacc cagacagtgg aagatattcg gtgcggcatg gacaggttat ttacttgatg
      241 gttttgattt cgttctgata tctctggttc ttacagaggt tcagcatgaa ttcggcttga
      301 cgacaataga agcggcaagc cttatttccg ctgcttttat ttcccgatgg tttggggggc
      361 ttgctattgg cgctttatcg gacaagatgg ggcgcaggat ggcaatggtt ttaagtatag
      421 tgctattctc tctcggaacc cttgcctgtg gtctggctcc tggatatgca gtgatgttta
      481 ttgctcgcat tgttattggg cttggtatgg caggtgaata tggctcaagt gtaacatatg
      541 ttatcgaaag ctggcctgtt catctcagaa acaaggcgag tggtttttta atttctggat
      601 tttctattgg gggaggttta gctgcacagg tttatagcat tgtagtacca ctatggggct
      661 ggcgttctct tttctttgtc ggcatgcttc caatattatt tgctttttat cttcgcaaga
      721 atcttccgga gtctgatgac tggcagaaaa ggcaacaaga gaataaaccc gtcagaacaa
      781 tggtggatat tctgtacaga gaaaagaata aatacataaa tattcttctg tcttgtattg
      841 catttgcatg tctatatgtt tgtttttcag gcgtcactgc aaacgctgct ttaatcacag
      901 ttatggcatt gtgctgtgcg gctgttttta tcagttttat atatcagggt atggggaaaa
      961 gatggccaac aggcattatg ttgatgcttg tggttatgtt ctgtttcctc tacggatggc
     1021 cactgcaggc ttttcttcct acatggctta aggttgatat gcagtattca ccagaaactg
     1081 ttgctctcat atttatgctg gcaggttttg gctctgcagc tggaagttgt ataggtggtt
     1141 ttatggggga ctggcttgga acccgaaaag catatgttat atcacttctt atcggtcagc
     1201 ttgttattat ccccgttttt cttgtggacc gggactatgt atggttactt ggccttctga
     1261 tctttactca gcaggttttt ggtcagggga ttggtgctct ggtgccgaaa ataatcagtg
     1321 gttattttaa tgtggagcag cgtgcggcag gactggggtt tatttataat gtaggttcac
     1381 ttggtggagc atgtgcgcct attcttggtg cagttgttgc gtctcataca agtcttggta
     1441 cagcaatgtg ttctcttgct tttatcttaa catttgttgt tcttgtcctt attggttttg
     1501 atatgccatc acgtgtacag agatggatcc atccagaagc tgcgcttgaa tatgatactg
     1561 tcgatggaaa acctttttat ggagcaagaa aaaagaatgt tgcaggagaa taacggatgc
     1621 tatcagggaa taaaaaaatg attcattaca gggagaatat atattctctt gataattatg
     1681 agcgttacat aactgacagt tcgctatttg agaatactga cgatggcata atatatacag
     1741 gtattgtgcc ccttattaca gttgttgagc ctgttgtatc taatcaacat agtgtgctga
     1801 ttatacctgg aggtggatac aggaggattg tttatgaaag agagggactt gatgtaggca
     1861 gatggatagc aggtcaggga tatacggcat ttattctttc ttacagaatg ccctccgtta
     1921 atggttatta ttcctttgcg gatgcgaaga atgccctgaa ctttatttct tactgggccg
     1981 gaatgtcgga gagaacattt aagagtgttg gatgtatggg gttttcggca ggtggtcatc
     2041 ttgctggata tattactcat ctccattatg aggaagagac ttctgtgcgt cctgactgga
     2101 tggctctgat atatccagtt atatctcttg acaggagtat tgctcatcag ggatgccgga
     2161 aacgtcttct ggatgcaggt gaaagtgaat attcagccag tcttgaaaat agtgttacag
     2221 agtatatgcc accagtcttt attgtgcatg caacagatga taaggatgtt ccagttattc
     2281 actctctgag atattttaac tctatccggg ataagggaca tcatgcagaa gctcattttt
     2341 ttgataaggg aggtcatgga tttggtgtat ccggaaaaaa tggatatccg atagaaaaat
     2401 ggcaaagcct gcttctggaa tggcttgcgg aacataagtt tcattgagaa atcagattac
     2461 aagtgataaa tatatttaat gatgaggcat ttatgaaaaa atattatccg ctattaactg
     2521 taattcttct tggtttacct ttgtgctctc aggcattaac tcttaatcta cgtgggggat
     2581 acagaagcgc cagtcacgct tatgaaacgt tgattcaggt tagtcagggg tggaataatg
     2641 gttggtgggc aagttttgag tcaaacacct ggaatacaat tcacaacaat gaaaatgaat
     2701 tgtttgctct caattataat aaaatagatg taaataaaac atataaaata gatgacaaat
     2761 gggctctaat cccagggatt cagacacaat ggatgtcagc aggaagtcag tataaccctt
     2821 atctgaaaat ttcgtatcag gcaacgccag atcttaatct ggccttacgc tatcgctatg
     2881 actggaaagc tttccgacag acggatttag atggcaacag ggcgcgtaat gatcagcatc
     2941 agtttgatgg ttatgtgaca tacaaaatta atgattcatg gttgtttgca tggcagacca
     3001 cggtatatac gaaggttaat aatttcaaat atgggaatca taaaaaaaca gcaactgaaa
     3061 atgcatttgt tctgcaatat aaaatgtcgc cggtgtttac cccatatata gaatatgatt
     3121 acctggataa acaaggggtg tataagggga aagataataa acatgaaaat agttaccgtg
     3181 tcggtgttct gattaatttt taaaaggtga ttacaatgaa agtgaaaaac tttatatatc
     3241 ttcccttctg cttgtttatt ggaacgtcgg tagccggagc tttaccggaa attcctgaac
     3301 catttaaata tggtgtagga gggatcgaga atggtaaaat atatatagga ctgggaagtc
     3361 tgggaaataa ctggtatatg atagatacaa accagagtga aaaaaaatgg actaagatag
     3421 cacaatggcc gacagttccc agagagcagg caacagctac tattattgat ggtaagatct
     3481 atgtatttgg aggaattgga aaagataaaa gtggggtcat aaccttacaa aaagatgtct
     3541 attcctatga tattgctaaa gataaatggg aaaaattgat gacccgtccc cctgtatctc
     3601 ttgccggtca tgtttctttt atccataatg gtcatgcggt atctactggt ggtgta
//
LOCUS       DAZFFI010000224         3555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFFI010000224 DAZFFI010000000
VERSION     DAZFFI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.226"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1156..3204
                     /gene="ireA"
                     /locus_tag="ACHJ12_004951"
     CDS             1156..3204
                     /gene="ireA"
                     /locus_tag="ACHJ12_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFO4992595.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1092 a          747 c          742 g          974 t
ORIGIN      
        1 agatcatcat ctgcctggtt tacacctacg ttcagtactt ttgtcacagc aaatgcaccg
       61 tcacttatct gcctgttcag ctctgcaatt tcctgtgcag aacaggcact cagcgcaaca
      121 cacgaagcac aaacgagtaa cattttttta gtcagcatgt cttcatcctc atcagcagct
      181 cttcagtgga taacatccac agttacacct gcatttacgc gcaacaaaaa caccagatga
      241 agattttatt cattcagctc cgctaaaaat taatcttgct gtggaatatc gggaaaacag
      301 gaagttacgt taaaagccga aggaaaaact cacaatccct tcttcaatca gcctctgaaa
      361 atcggctttt cagcatgtct tcccccattg ttcgtatacc ctgttcgatg gattattatt
      421 gatccatatg atcgcttcac gtaaacacga ttctggctcc acaccattct ggacgcgatt
      481 gttaccaaaa aagacaacca tcacaagacg tcacagacgg tgcgtgcgct tactcctgta
      541 tatctggcgc aacagaatct accagtgtaa actctctatg cttttgcgca gaagcagtgg
      601 gcctgggttc gcttatgtcc tgcatctaca agcgaatgac tgaccagaga ctcccatatc
      661 tggtcagtcg cccattttta ttttccctat acactcagac tacaatagcc gtaacaaaca
      721 gcataccccc agtcatagtg gcccctttat cttcttatgg cttgtctgtg gtactggaga
      781 tagagcaact gtgggagtac tgttatggcc cccggtaaga aaaactcttg cttaatgtac
      841 agcagaactg ttcccagaaa gaagcctgtg catattttca tagaaggaga tcacccataa
      901 cggcgttttt cacggtaata cacgccaggt aaagaatgct ggattatcaa tcaaagctcc
      961 gcacaggaag cgcttatgcc gtagagcgcc ctctggagaa aagtgctact tcttgtcgat
     1021 tcagactagt tcaggaaatt atacgaaaat attacctgaa tgagctaaat gttatttacc
     1081 ttcctttctg ttcattgtaa attttttctt gctaacgcaa atcattatca ttacgatttt
     1141 agaaaaggga aatttatgaa gaacaaatat atcattgctc cgggcattgc cgtgatatgt
     1201 tctgcagtca tatcatcagg ttatgccagt tctgataaaa aagaagatac gcttgttgtt
     1261 actgcctccg gcttcactca gcaactcaga aatgcaccgg ccagtgtttc agtcattact
     1321 tcagagcaac tgcaaaaaaa accggtttca gacctggtcg atgcggtaaa agatgttgaa
     1381 gggattagta tcaccggtgg gaatgaaaaa ccggatatca gtatacgtgg gttaagtggc
     1441 gattatacat tgattctggt cgatggacga cgtcagagtg gtcgggaatc cagaccaaac
     1501 ggcagcggcg gttttgaagc cggatttatc cctcctgtgg aagcaattga gcgtattgaa
     1561 gttatacgtg gccctatgtc ttccctgtat ggttccgatg ctatcggagg agttattaat
     1621 atcataacca aacctgttaa taaccagaca tgggatggcg tacttgggct tgggggaatt
     1681 attcaggaac atgggaaatt tggtaactca accacgaatg acttctacct gtcaggccca
     1741 ttggttaagg ataaacttgg tcttcagtta tatggaggaa tgaactatcg caaggaagat
     1801 agtatctctc agggaacacc agcaaaagat aataagaata taacggcaac actccagttt
     1861 actccgactg aaagccagaa gtttgttttt gaatacggaa aaaataacca ggaacataca
     1921 ttaacacctg gtgattctct tgatgcctgg actatgcggg gaaatcttaa acaaccaaac
     1981 agtaaaagag aaacacataa ctcacgtagt cactgggtag cagcatggaa tgcccagggc
     2041 gaaatactgc atcctgaaat tgctgtttat caggagaaag ttattcgtga ggttaaatct
     2101 ggtaaaaaag ataaatataa tcattgggat cttaattacg agtcaagaaa accggaaata
     2161 accaacacga tcatagatgc aaaagtgacg gcatttctgc cggaaaatgt gctgaccatc
     2221 ggaggtcaat ttcagcatgc agaactccgt gatgactcag ccacaggtaa aaaaacgaca
     2281 gaaacacagt ctgtttcagt taaacagaaa gctgttttta tagaaaatga atatgctgca
     2341 acggattctc tcgccctgac tggagggctg cgcctcgata accatgaaat ctatggcagt
     2401 tactggaatc caagattgta tgctgtttat aacctgaccg ataatctcac tctcaaagga
     2461 gggatcgcaa aagcatttcg ggccccttcg attcgtgagg tcagtcctgg atttggaaca
     2521 ctgacgcagg gtggtgcctc tattatgtat ggaaacaggg acctgaaacc ggagaccagt
     2581 gtaaccgaag agatcggtat tatttataat aatgacagtg gtttttcagc aagcgcgact
     2641 ctgtttaata cagattttaa aaataagctg accagttacg atataggcac aaaagatcca
     2701 gtcactgggc taaacacttt tatttatgat aatgtgggtg aggcaaatat cagaggtgtg
     2761 gagcttgcaa ctcagattcc tgtgtatgat aaatggcgtg tatctgcaaa ctatacattc
     2821 actgactccc gtcgagaaag cgatgacgaa agtctcaatg gcaagtcgct gaaaggggaa
     2881 cctctggaaa gaactcccag acatgcagcc aatgcaaaac tggaatggga ttacactcag
     2941 gatattacat tttattcatc tctgaattat acaggaaaac aaatctgggc agcacaaaga
     3001 aatggtgcca aggttccccg tgttcgtaat ggattcactt ctatggatat tggtatgaat
     3061 taccagttcc tgccaggcac gctgattaat tttgccgtac ttaacgtcac agacagaaag
     3121 agcgaagata tcgataccat tgatggtaac tggcaggtcg atgaaggacg tcgttactgg
     3181 gctaatgtaa gaatatcctt ctgagagaat ataggtaacc tgaggccgtg cgcctcaggt
     3241 accagtatag taatcagtaa cgtcagcttc caggttgtaa tgcttttaat tttgataaaa
     3301 ataaacttaa taaattacat ttgttaaata ttgataattt taatttggtt ttattactcc
     3361 agttatactc acactctatc tttacacaat tcatcaccat tacacctcca tagcctgttg
     3421 atttaaaaga tttaatttcc tgtgaaacaa tcaggcctgt atgaacctga ttataccaca
     3481 taatctttta acttatttaa atttaactct caggcttttc tgtcagtaaa atcagaaaga
     3541 attgcaggat ttcgt
//
LOCUS       DAZFFI010000225         3456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFFI010000225 DAZFFI010000000
VERSION     DAZFFI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.227"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..906
                     /locus_tag="ACHJ12_004952"
     CDS             <1..906
                     /locus_tag="ACHJ12_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4992596.1"
                     /translation="NIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKI
                     MKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQ
                     TQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNTGD
                     SLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRT
                     PASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKAESTLFLE
                     KRRIYQWMLSPFYDMKHSATGPLND"
     gene            899..3001
                     /gene="mchF"
                     /locus_tag="ACHJ12_004953"
     CDS             899..3001
                     /gene="mchF"
                     /locus_tag="ACHJ12_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO4992597.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TKMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(3062..>3456)
                     /locus_tag="ACHJ12_004954"
     CDS             complement(3062..>3456)
                     /locus_tag="ACHJ12_004954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992598.1"
                     /translation="FFRYLRVYCFYLFYLSLIKKGNNMRNITTDELALIHGAGLDKDA
                     SVAIGTAAGGFLGKATKIPSADIAGSAIGGYIGGVIADGSNRTITIPSNGINYNPSIG
                     LGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT          991 a          679 c          823 g          963 t
ORIGIN      
        1 aacattattt cccgtctgga agaaagtaaa aaaataacgc tggataccct ggaaaaacaa
       61 cgtctgcaat acacagatgc gtttcgtcgc tcatcagata ttatacagcg tgcagaggaa
      121 gggataaaaa taatgaaaaa caatatggag aattacagaa actatcaggc aaaagggctg
      181 attaataaag atcagttaac taaccaggtg gcattatatt atcagcaaca aaacaatctt
      241 ctcagcctga gcggacagaa cgaacagaat gccctgcaga taaccactct ggagagtcag
      301 attcagactc aggctgcaga ttttgataac cgtatctacc agatggaact gcaacggtac
      361 gagttacaga aagaactggt taacactgat gtggagggcg aaatcattat ccgggcgttg
      421 actgacggga aagttgactc cctgagtgtc actgtcgggc aaatggtcaa taccggagac
      481 agccttctgc aggttattcc tgagaacatt gaaaactatt atcttattct ctgggtcccg
      541 aatgatgctg ttccttatat ttcggctggt gacaaagtga atattcgtta tgaagccttt
      601 ccggcagaaa aatttgggca gttctctgct acggttaaaa ctatatccag gactcctgcg
      661 tcaacacagg aaatgttgac ctataaggga gcaccacaga atacgccggg cgactctgtt
      721 ccctggtata aagtcattgc gatgcctgaa aagcagatta tcagatatga cgaaaaatac
      781 ctccctctgg aaaatggaat gaaagccgaa agtacactat ttctggaaaa aaggcgtatt
      841 taccagtgga tgctttctcc tttctatgac atgaaacaca gtgcaacagg accgctcaat
      901 gactaacggg aatttcagac aaattataaa tcagcttgat atgcgctggc gacgtcgtgt
      961 tccggttatt catcagacgg agaccgctga atgtggactg gcctgcctgg caatgatatg
     1021 cggtcatttt ggtaagaata ttgacctgat atctcttcgc cggaagttta atctctcggc
     1081 ccgtggagca aaccttgcag gaatcaatgg aatagcggag cagctgggga tggtcacccg
     1141 ggctctttca ctggagctgg atgaacttgg tgccctcaaa atgccgtgta ttctccactg
     1201 ggatttcagt cacttcgtcg tgctggtcag cgtaaagcgt aaccgttatg tactgcatga
     1261 tccggccaga ggcagaagat atctcggtcg ggaggaaatg agccggtatt ttacgggcat
     1321 tgcacttgag gtctggcccg gaagtgaatt cctggcggaa acccagcaga cccgcataag
     1381 tctccgttca ctgattaaca gtatttacgg tattaaaaga acactggcga aaattttctg
     1441 tctgtcagtt gtaattgaag caatcaatct ggtaatgccg gtgggaactc agctggttat
     1501 ggatcatgcg attccggcgg gggacagagg gctgctgacg cttatttctg ctggcctgat
     1561 gttctttata ttgctcaggg ccgcggtgag tatgctgcgt gcatggtcct cactggttat
     1621 gagcacgctc atcaatatac agtggcagtc gggtctgttt aaccatcttc tcagactgcc
     1681 gctggcgttt tttgaacgcc gtaaattagg tgatatccag tcgcgttttg gctcccttga
     1741 cactttgagg gccaccttta ccacctgtgt tgttggggca atcatggaca gtattatggt
     1801 tgtgggtgtt tttgtgatga tgctgttata tggaggatat cttacctgga tagtgctcgg
     1861 ttttaccatg gtttacgttc ttattcgtct ggtgacatac ggctattacc ggcaaatatc
     1921 ggaagaaact cttgtcaggg gggcccgggc cagctcctat tttatggaaa gcctgtatgg
     1981 tattgccacg gtaaaaatcc agggtatggc tgggattcgg ggaacacact ggcttaacct
     2041 gaaaatagat gcgatcaatt caggtattaa gttaaccaag atggatttgc tcttcggggg
     2101 gataaatact tttgttgccg cctgtgatca ggtggcgatt ttatggctgg gtgcaagcct
     2161 tgtgatcgat aatcagatga caataggaat gtttgtggca tttggttctt ttcgtgggca
     2221 gttttcggat cgggttgctt cgctgaccag ttttcttctt caactgagaa taatgagtct
     2281 gcataatgag cgcattgcag atattgcact acatgaaaag gaagaaaaga aaccggaaat
     2341 tgaaatcgtt gctgacatga gcccggtttc actggaaacc actgatttaa gctaccggta
     2401 tgacagccag tcagcacagg tattcagtgg tctgaatttg tctgtggctc cgggagaaag
     2461 tgtggctata actggtgcct ccggtgccgg aaaaaccaca ttaatgaaag tattatgtgg
     2521 actgtttgaa ccagatagtg gaaaagtact ggttaatggc acggatatac gtcaacttgg
     2581 aataaataat tatcaccgta tgatagcctg tgttatgcag gacgaccgac tattttcagg
     2641 atcaattcgt gaaaatatct gtgggtttgc agaagaaaca gacgacgaat ggatgacaga
     2701 atgtgccaaa gcaagtcata ttcatgatgt gataatgaaa atgccaatgg ggtatgaaac
     2761 gttaataggt gaactggggg aaggtctttc cggcggtcaa aaacagcgta tattcattgc
     2821 ccgagcttta taccggaaac ctggaatatt atttatggat gaggctacaa gttctcttga
     2881 tacagaaagt gaacgtttcg tgaatgctgc tataaaaaag atgaatatca cccgggtgat
     2941 tattgcacat agagagacaa ctttaagaac agttgatcgg atttttagaa ttacaaatta
     3001 ggataatata tcactaaaag cagcattgct gcataataag ctatgctgct ttagaaatta
     3061 atcaacttcc actactagaa gaggatgttg atgagttaaa tcctgagttt aataaacctc
     3121 cattgtagtt tggattaaaa cttccaagtc ctatactcgg attatagtta ataccgtttg
     3181 atggtattgt tattgtccta tttgaaccat cagcaataac tcctccaata tatcctccaa
     3241 ttgcacttcc tgctatatcc gcactaggga tttttgttgc tttacctaaa aaaccaccag
     3301 ccgcagtacc tattgcaacg gatgcatctt tatctaatcc tgcaccatgt attaaagcaa
     3361 gttcatcggt tgttatattt ctcatgttat ttcctttttt tattaaagaa agataaaaca
     3421 aataaaaaca gtacactcgc aagtaacgaa agaatt
//
LOCUS       DAZFFI010000226         3446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFFI010000226 DAZFFI010000000
VERSION     DAZFFI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.228"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..112
                     /locus_tag="ACHJ12_004955"
     CDS             <1..112
                     /locus_tag="ACHJ12_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992599.1"
                     /translation="AAKVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
     gene            136..438
                     /locus_tag="ACHJ12_004956"
     CDS             136..438
                     /locus_tag="ACHJ12_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992600.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTTVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            594..881
                     /locus_tag="ACHJ12_004957"
     CDS             594..881
                     /locus_tag="ACHJ12_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992601.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEAEHFKQFLRQIKRTGLDIRAIC"
     gene            1000..1821
                     /locus_tag="ACHJ12_004958"
     CDS             1000..1821
                     /locus_tag="ACHJ12_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4992602.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVPGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRLEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(2118..2708)
                     /locus_tag="ACHJ12_004959"
     CDS             complement(2118..2708)
                     /locus_tag="ACHJ12_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO4992603.1"
                     /translation="MVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFINVTC
                     KAGDCFDLAGRDYKIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            3041..3424
                     /gene="traM"
                     /locus_tag="ACHJ12_004960"
     CDS             3041..3424
                     /gene="traM"
                     /locus_tag="ACHJ12_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO4992604.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
BASE COUNT          984 a          699 c          828 g          935 t
ORIGIN      
        1 tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaaa ggcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca
      121 gatacaggag gaaacatgag cgcattttct tttaacaccg cttatcagcc aacaggcgat
      181 cagcagaaag acgcaatcgc acaaatcgac atcatgcaga atcgtgctgt tcaggcaaat
      241 cttgcatacc agagcagcct ggatgcggaa acgctattga aacagttatg gcaaatcaat
      301 gatgaactgg gggaactgtt ggatagcgtg gaaaatcaca caacagttat cagaaaaaaa
      361 gccagtgatt tgagcgcact gatgatgctt ttcagccaac aggccggtca gcctatgact
      421 atgttgttga tttgttaatg ataatcaggg gcaggaaatt aagcgaaaga tgctctgccc
      481 cgggtttgca gcgaccggaa cggtcacgcc acccttgtcg cgctgtggcc tgcggcggct
      541 cccgaaactc aaccgatgat ggggtctgaa aaccagagag aggagaaagc aggatgagta
      601 cccgaaatat ccacgttaac accgcgtcgt acacgcttct tgttgccggg aagaaaaaaa
      661 acaccgggga agagtgggac gtcctggaat tcagcagcct gactgagctg aaaaaatatc
      721 gcaaaagcca cccggaaaag atggccttca gctatagcta cgcgctcagc cggggtgtgg
      781 atacgcagtt ccgccatatc aacattgcgg aagccgaaca cttcaaacag ttcctgcgtc
      841 agattaagcg taccggcctc gatatccggg cgatctgctg accggatgtg atgaatatcc
      901 ctcctgattg ttgcaatctt gttaatggag accgggaggg atgatattaa gcggaaaaat
      961 tttttattaa atttattctt tcataaagga gtagctgtta tgcgattagc ttcccgtttt
     1021 ggtcggtata attccattcg ccgtgagcgt cctttaacgg atgatgaatt aatgcagttt
     1081 gtgccttcgg tattttccgg tgataaacat gaatcccgga gtgagcgtta tacatatatt
     1141 ccaacaatca atatcatcaa taagttacgt gaagaaggtt tccagccgtt ctttgcatgt
     1201 cagagtcggg ttcgggattt gggacgccgc gaatacagta aacatatgtt acgtcttcgc
     1261 agggaagggc atattaacgg gcaggaagtt cctgaaatta tcctgcttaa ttcacatgat
     1321 ggttcatcca gttatcagat gatcccagga atttttcgtt ttgtctgcac aaatggtctg
     1381 gtgtgcggaa ataattttgg cgaaatccgc gttccacata aaggcgatat tgtcgggcag
     1441 gttattgagg gagcgtatga agtgcccggt gtctttgata aggtcactga aaatatggag
     1501 gcgatgaaag aaatacatct taacagtgac gagcaacatt tatttggcag agctgcactg
     1561 atggtcaggt atgaagatga aaataaaaca ccagtgacgc ctgaacaaat aattactccc
     1621 cgtcgtttgg aggataaaca gaacgatctc tggacaacct ggcagcgggt tcaggagaat
     1681 atgataaaag gtggactgtc ggggcgaagt gcctccggta aaaataccag gacaagaggc
     1741 attacgggta ttgatggtga tatccgaatc aacaaagcat tatggatgat tgccgaacag
     1801 ttcagggagt ggaagtcatg ataatataag taaagccccg aaaatttttt cggggcttta
     1861 cttatattat cattatgcgg cattgatatg tcgtctgaga tgcctcaatt tcatcaactg
     1921 gcttttcggt agtctgtaaa tattgtgccc gcgaaaaatg gtctggcagg aggctgcgca
     1981 gttaccggat atatataaga tgggttcccg ctttgcgggg cttcggccag tcagcattat
     2041 cactcatatg atatttttgt gtgtggcctt ccatgccgcg tttgcggcat aaccggtttt
     2101 ttaaaatgta tatcaaatta attcttctgc gagctgttca tttttgaaag cggtttttca
     2161 gatgcacgaa gctggatccc cttgctgttt tttatcccgc tgtaaatccg gtaaacctct
     2221 gatgcgtagg caagacgtct ctggttctgg cgttcggtct tcctgaatcc ggcattgtat
     2281 gcaccaacag cctcccagga gactccccat tttttaaagg ctatagccag ataataagca
     2341 ccggtataaa tgttcagaca gggatctgct gtcagatgtt ccggtttaat tccgtagcgg
     2401 gccagttcgt taaaatgctg ggaatctacc tgcataagtc cactgccata tcccgtaacc
     2461 ggattaatac cgatggcatt aacccggtta cgggattctt tccatgatat tgctctcagc
     2521 aagtcagggt caattttata atctctgcca gccagatcaa agcaatcacc agccttgcag
     2581 gtcacattta taaacatcag gcagatcgtc agaagccatt ttctcatttt ctcacctctg
     2641 gtgacgtcat caggaaataa tttaacccac tccacaaaaa ggctcaacag gttggtggtt
     2701 ctcaccacca aaagcaccac acactacgca aaaacaagtt tttgctgatt ttcgtttaat
     2761 atcattgagt tgtatttgcg gatttatatt gtttaaatct gatttattta aaacagcgtc
     2821 gttaacgccg ctacagcaac gcgcagacac cgctttttag gggtggtact gactattttt
     2881 ataaaaaaca ttattttata ttaggggtgc tgctagcggc gcggtgtatt tttttatagg
     2941 ataccgccag gggcgctgct agcggtgcgt ccctgtttgc attatgaact ctggtgtttc
     3001 taaattaact ttattttatg ttcaaaaaag gtaatttcta atggctaagg tgaacctgta
     3061 tatcagcaat gatgcttatg aaaaaataaa tgcgattatt gagaagcgtc gacaggaagg
     3121 ggcaagagaa aaagatgtca gtttttcagc aacagcttca atgcttcttg aactggggct
     3181 tcgtgtatat gaagctcaga tggagcgtaa agagtccgcg tttaatcaga ttgagtttaa
     3241 taaattactt cttgaatgcg ttgtaaaaac acaatcatca gtagcgaaaa ttttgggtat
     3301 tgagtctctc agtcctcatg tttccgggaa tccaaagttt gaatatgcca atatggttga
     3361 agatatccgg gagaaggtgt catctgagat ggaacgattt tttccaaaaa atgatgagga
     3421 ataaaaaaga attaaaagtt gatttt
//
LOCUS       DAZFFI010000227         3362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFFI010000227 DAZFFI010000000
VERSION     DAZFFI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.229"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..434)
                     /locus_tag="ACHJ12_004961"
     CDS             complement(60..434)
                     /locus_tag="ACHJ12_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992605.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            1153..2238
                     /locus_tag="ACHJ12_004962"
     CDS             1153..2238
                     /locus_tag="ACHJ12_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO4992606.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHQKKRAGSKNS"
     gene            2513..2698
                     /locus_tag="ACHJ12_004963"
     CDS             2513..2698
                     /locus_tag="ACHJ12_004963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992607.1"
                     /translation="MIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASM
                     TLMLKEMSNLINDEGIK"
     gene            2711..2830
                     /locus_tag="ACHJ12_004964"
                     /pseudo
     CDS             2711..2830
                     /locus_tag="ACHJ12_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            2790..3362
                     /locus_tag="ACHJ12_004965"
                     /pseudo
     CDS             2790..3362
                     /locus_tag="ACHJ12_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         1069 a          648 c          753 g          892 t
ORIGIN      
        1 cggaggaccg gatgggaatc gggagacctg gacaaaaagt gcaggcctcc atgacacggt
       61 caggacagtt tgtgtttaac cagaatattt agcgcctgta ttggggcttc gtggagctcc
      121 cgcagacatt cagggtcagt aacatgttcc tggtatcttg ttttctcaag taacaagcgg
      181 tgcatatgca gcatacgggc aagagccgcg cacacctcct caaatgcggt atcccgctta
      241 ccgtcactgt cagagccaca aaacagcggt acaacctcca cgccgtcgct gtgggccttt
      301 agccaggcat caaccacggt taaatcatcg gtgaagaacc acgcactgtt agcgctgaat
      361 cgcattttcc aggcttccgg tactggttgc ggggagtatg aatatgctgg tggtatcttg
      421 tcgtgagtat ccataggcta tcgttccgtc attctgttat tcaccacagc gctaaaaacg
      481 ccatgaagga gcagaatacc gattgcctcg tttgctgtat gaggtgctta ccacacattg
      541 ggtacacaga tgaatcaggc ggaagtgtga ttaccggaaa aaatccattt cagtttgagg
      601 gaaaaaaaac cgtagtgcga taacactacg gcatgccacc aaagaaacac aacaagaggt
      661 caacaatgag taatggacat gaccagacga ttttatcatg ccggaacaaa agttatgtga
      721 cacagtcgga taaaaacagg taaagcccac cagcttcata ggggataacc ggaaaaattc
      781 cggtaaatat ttgcctgagt aagtcatcaa gcgcctgcta aatgtgtata gaaatgtgta
      841 taggattgat tttagaacat gtggaaatgg ctttattact ggtggcatgg cttgttgttc
      901 tgttcccttc gccgctccaa tttatcttca cctgatcaat ctgccatttt tgctgtatct
      961 gattttctat attatcttaa ttcaatcgct tcagactttt cgcaatcgcc gtaatacttc
     1021 cccattttgt tgtaaataaa aagcgtttac gcaatgcaat ccttttgtaa caacccaggt
     1081 aaaacacctt attttcaagt tgttaaataa gtggttatct acgcttttaa caaacgattg
     1141 atggaatatt ttatgaaagg gaaatctgca ttaactcttc tgctcgctgg tatattttcg
     1201 tgcggcacat gccaggcgac aggggcagaa gtgacgagtg aatctgtatt taacattctt
     1261 aattccacgg gggcagcaac agataaaagt tatttatcgt taaatccaga taagtatcct
     1321 aattatcgtc ttttgattca ctcagccaaa ttacaaaatg aaataaagag ccactatacc
     1381 aaagacgaaa ttcaaggatt gctaacatta acagaaaaca cacgaaagtt aacgttgacg
     1441 gagaaaccct ggggaacgtt tattcttgct tctactttcg aagatgacaa aacagcggca
     1501 gaaacccact atgatgctgt ctggctgagg gatagtttat ggggctatat ggcattagta
     1561 tcagatcagg gaaatagtgt agccgcaaaa aaggttctgc tcaccctgtg ggattatatg
     1621 tctacgcccg accagattaa gcggatgcag gacgtcatta gcaaccccaa acgactggat
     1681 ggagttcccg ggcaaatgaa tgttgtgcat atccgatttg acagcaactc ccccgtgatg
     1741 gcagatgtgc aggaagaagg caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta
     1801 ggtctttatc ttgatctcct tatacaagca atcgacactg atacaattaa cgctgaagat
     1861 tggcaaaaag gtgaccgatt aaaatcggtt gcgctgctga ttgcttatct ggataaagcc
     1921 aatttctatg ttatggaaga ttccggagca tgggaagaag atgcccgatt aaacacatct
     1981 tccgttgcac tggtgacctc tggactggaa cgtttgtcga acttactctc gaagaaagac
     2041 tctgtttttg tctctgattt actacgcgaa gctaaagcca atgaactgga tgaacctctt
     2101 tcaacaacga ggttgaacca cctcatagat aaaggttatg aaagaataac gttacagctt
     2161 gatttagggg gagaatcccc aggttatctg gaaaaagata aacaccagaa gaaaagagca
     2221 ggttctaaaa atagttaaaa aactagcagg tccgtatggt ataaaaagat acgagaagga
     2281 taattatcag tcggcaaatt tttggttcaa tgatattaaa accgataccg atcaaaattc
     2341 acatgcgaaa agagaaaaga gcttcattcc atcaacagaa gcagaatggt tttttgactc
     2401 atggtatgca aaatcagcag caattgttta taaagaatca agaaaggaag agtatttgaa
     2461 tgactcagtg caatttatga atagatcatt ggcccaaata acaggcgaga atatgattgg
     2521 tgcaaatggc aggagtgtac ctgaaatggc acttcccgaa agctataact atatccacaa
     2581 gtcgggaact ttgcatgaag cgccaagccc tatcattccg ctaaattggt cgaaagcttc
     2641 aatgactctc atgttgaaag agatgtccaa ccttatcaat gatgagggaa ttaaatagtt
     2701 ttcataatga gttacagcac aacgttgggt attgactgag caagatgaac gacaaggaca
     2761 acgacatcag ccttatgagg agcaagaaaa tgaaagcaaa ttcgaaaacg atcagaagga
     2821 tgaaagttaa tgttcggtct gaggaaagta aatcgaaaca ctcatacgtt tgaaaccact
     2881 tttcaaaatt ggaaagaaaa tggcgaagat gttgcattat taatgcccga atttagtgct
     2941 aaatggttac cgatagcaga agagagtggc agctggtctg gatgggtatt gcttggagaa
     3001 atatttccct taatttcatc tgagctcgct gggatggctt taatgccaga aacggaaagg
     3061 ttaatagggg aatggctcag tttatccagt tccccgttaa atctaaagca tcctgaacta
     3121 aaatataatc gtttgcacgt aggtaaagta tttgatggag tattgagccc tgctcaacca
     3181 ctaataagaa tatgaacagg ggaattgaat ctttggttag ataaagtcac agtctgccaa
     3241 tacggaaacg ctccagtgtt agacaaaaaa tcattatatt ggtccattca ttttgtgatt
     3301 ggatttagta aaacatgtta cagaagtctt gttgatattg aagttggtga tgttttatta
     3361 at
//
LOCUS       DAZFFI010000228         3340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFFI010000228 DAZFFI010000000
VERSION     DAZFFI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.230"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..316
                     /locus_tag="ACHJ12_004966"
     CDS             122..316
                     /locus_tag="ACHJ12_004966"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4992608.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            735..962
                     /locus_tag="ACHJ12_004967"
     CDS             735..962
                     /locus_tag="ACHJ12_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992609.1"
                     /translation="MSVDVIKQIQSIQDKGIIIYSKYRANEFNRDDVYRESYSLIIEF
                     NHLIAEHAVHDEKLINHTVCILNELRSIAIN"
     gene            complement(1621..2637)
                     /locus_tag="ACHJ12_004968"
     CDS             complement(1621..2637)
                     /locus_tag="ACHJ12_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO4992610.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAERPAARWLFMTLTVKNPPVEDTRETLIRMNAAWNRLVRRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT          938 a          843 c          727 g          832 t
ORIGIN      
        1 gaaaatcacg ttaaaacgat tacgagcgtg cagttgggct gatatatcaa cagtcatgcc
       61 aatatgtatt aacccgacag gccgctcttt tttaattcaa ataaaaatat aaattacgag
      121 tatgaatcag gacaaagcaa tttatttaga aaataaatat ttttcacccg caaacgaagc
      181 tgtcagactg gagcgtgaag gcgattacat tggagcatgg aaaaaatggc atgatgcgct
      241 tcattatgcc aaaggcgaaa atatagattg gtgtatagcc agagctgaat attgtaaaaa
      301 gacaggccgg ttttaattct gacggggttt caaaggggcg tcccctttgg cacgtcgacg
      361 tatcaaaatg cgccagcatt ttgtatacag tcggtcacgt gcctccagga tttaccacta
      421 aaaacgcccc tgtaattaat cctgaatcaa agtataacta agatataatt agggtataac
      481 taaagcataa ctaaagtata actaagttag tgtgcatata attaatatat aactaatgta
      541 taactaacga ttcaccaacg gatcgcatgt agtaggcgct acataaaaaa tcctgaaatg
      601 gaggattcgt cataaagaga ttcctgtttt tagcgttctg atgcaggata gggcaaacac
      661 cgcaaaatga cgtctttggt atgttttgtg atgtttgctt ctggcatctg ttatctgttt
      721 tatagggtgt ttttatgagt gttgatgtaa ttaaacaaat tcagagtata caggacaaag
      781 gaattattat ttattcaaaa tacagagcta acgaatttaa cagagatgat gtatacagag
      841 agagttacag cctgattatt gagtttaatc atcttattgc tgaacatgcc gttcatgatg
      901 aaaaactgat taaccatact gtctgtattc tgaatgaatt acgaagcata gcgataaatt
      961 aaaaaaatcc ccctggatta cagggggaag ccatcagagt ttgagtaatg agaaatatta
     1021 ttttggagag agcaccacaa atgcgctctt ttccaatctg ccgtaaccat cagcaaaaga
     1081 caatcagaac aggcgaaaaa atccccctat atggcaaggg ggtgtcaatg aggacatggc
     1141 aattgtgagt tttgtgtgtt ttatgtgttg cagtttgcaa cgtcagaaat ttgccgcagt
     1201 tatgcgcgaa aaacaatcct gatttcagga caggatgtgt aatcgtataa ccgcgcacgc
     1261 acggctatac gattaccacc tggtcagggc tttgcccgac accctcgaca ccgttcccgc
     1321 aatacgatag aacatcaggg cgcgtttccg ggtaaacgca tgttaatgac cgggaattca
     1381 ttgattaggg accggagaca cgctacaaaa ctcctgcgcg tttctggcat caactgacca
     1441 tggggatggt ttggcagtag cagcaagcgc cagaaacgcg ctcaaaacgt ctctgacggc
     1501 ctgaaccaac caaaacatac aaaaccattg cccaatgcaa aaaagtgcgt tagaaagcgt
     1561 ctgagcgcgt ttctggcgtc ttttttgttg ctgtgtcgct actcgttgtc agattcgcgc
     1621 tcaatccttc atcctgtcag cttcaggatt ccgcttgtaa cgtttttccg tacgtcccca
     1681 gtcaaacgcc agccagaagg cttgctcctc gaactcgtcc agttctgccg gaatatctgc
     1741 cccgatcaaa tcctcgtccg tctccggttt atcgactttc agaacctctt tgagacgacc
     1801 gccggttgcc accatgcgaa gcccataggt ctgccgcgcc agctccagaa accacgccgg
     1861 atcacgcaca acttccgaag atttcaccgt gtatttcatc gtctcaatga cgcctgactc
     1921 cagcgccgca cgatgcatat caggcagggt tacaccatcc ggccgtttcc agccctgttt
     1981 cggttttacc acccgaatat ccacacccgc ctcataatcg acacgaaggc attcactcca
     2041 gatctccacc cagcgagcat gtttcacata accattaccg gacaacatcg aaggggggac
     2101 catcatcaga acgtgaaaat gcggatgaca acatcctggc accttcccgt aagtaatttc
     2161 cgtcgtacgt aaccagccaa gaactgacct gaattcttta cgccgaacca acctgttcca
     2221 cgccgcattc atccgaatga gtgtttcgcg ggtatcttcc acagggggat ttttgacagt
     2281 cagcgtcatg aacaaccagc gggccgcagg acgttcggcc agcagctttg gcaaggcctc
     2341 ccagaggcga cgcatccagc ggagagaacg acgacgacta cacaccggac aaaacgggac
     2401 atgacaaaac tcccccttcc gaagggccag cttcatttcg ccagtcaggc gggacacctt
     2461 cggcgcaaac aacaaccgac gggaacaatc aaacatccgt tctccacgag aggaaaacat
     2521 cgaatcgctt tcgtaaatct gcgccaccct gtcagccatc actcgatcac tgtcccattt
     2581 tgtatcccgc gtgctgtaat cgcgtaacga gagtaccgga ctgtctggat ttttcatttg
     2641 caccccccaa gaactgggca gatgcggaaa ttttgtgtta gcatttttct gtcacctgtg
     2701 actctgttgc cgaacaacga attccaaact ggtgatcgtc ccctggagtg ttaccgcact
     2761 cgcaggggaa ccccctcaaa atcgttcatt ttttaaccag aagtttttat ctcttttcca
     2821 cccattttta ccgctcaaag ccgcgccacc actgggctaa gccaaaataa cacatccttg
     2881 agttgttgcc tatagtataa atacaaggat cgcgcgcgcg atcgcccacc aaaaatgcgc
     2941 gttttttgag cgcttttccg cacaacaaaa caacacacaa aaaccactct cccgacaata
     3001 ctccagcagg ttttctcacc gattttccga acaacaaaac cgctttgcag gtggttggtc
     3061 ccactgcgtg acaccaacca ccatcaccag tgttttttct cgtaccgtcg tcaatcacaa
     3121 aatcggaacc cacgccggag gcgcgggacc ccacgatttt ctgtttgacg agtcagaaaa
     3181 cgacgaacgc tgacagattt caatctggct gcgtgagacg ttggcggggt gtcggggcgc
     3241 agtcctgacc aggcggcatt tgtctgattg cgtgtgcgcg gtccgacaaa tacacatcct
     3301 gtcccgtttt tcgcacggtt tttattgatt accggacaga
//
LOCUS       DAZFFI010000229         3314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFFI010000229 DAZFFI010000000
VERSION     DAZFFI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.231"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..376)
                     /locus_tag="ACHJ12_004969"
     CDS             complement(<1..376)
                     /locus_tag="ACHJ12_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4992611.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKPYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGN"
     gene            complement(469..852)
                     /locus_tag="ACHJ12_004970"
     CDS             complement(469..852)
                     /locus_tag="ACHJ12_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992612.1"
                     /translation="MGLDIYFLARDNHHSGVVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(968..1597)
                     /locus_tag="ACHJ12_004971"
     CDS             complement(968..1597)
                     /locus_tag="ACHJ12_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992613.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(1679..2056)
                     /locus_tag="ACHJ12_004972"
     CDS             complement(1679..2056)
                     /locus_tag="ACHJ12_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992614.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(2252..2710)
                     /locus_tag="ACHJ12_004973"
     CDS             complement(2252..2710)
                     /locus_tag="ACHJ12_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992615.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(2793..3185)
                     /locus_tag="ACHJ12_004974"
     CDS             complement(2793..3185)
                     /locus_tag="ACHJ12_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992616.1"
                     /translation="MSTFILCPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT          887 a          969 c          771 g          687 t
ORIGIN      
        1 ggttaccgtg ttcatccaca gcctgttttt tccagtcttt gtaaatcacc gccagcgtgg
       61 ccacctcccc tttacggacg tggccaccca gttgttgcgc ctgacggtac gtcagccagt
      121 cattgctgac atacccggct tcatccgcgg ccagccacag caacaggacg ttcacccctg
      181 tataaggctt cccggtcaga gcatttgccg gcatcatctc cggaagcccc acgccattac
      241 gcgcagagcg ccagggacgt ttccagggtg gcacaccatt ttcaatggcc gtaacaaccc
      301 tgtcggtcac aagctgatac aggtcacgtt ccggagtttt agtcgcggta cgtgaagtat
      361 gttttttcgt tttcataaga gcatgccccc tgaaaaagat gcacgctccc tgtacggtga
      421 gagtgcaccg tacagttaaa ttaataacga aaatcagact ggccagaatt accacaacgc
      481 cagcatcacc agtgaacgac gctcaaaatc aaacgtcttc agcgtattgc tgacccacgc
      541 ttttacgtca tccacgtcat accagtaatc accggcataa tcttcccccc gtaaacacga
      601 gcctgtgaca taaggaaaca gcctgtggca gttccccggt gtcagcatac tgaggtcgcg
      661 caataatgca gccagtgaac tgcgggttac agggaaagca tgctgctcat ctgaagcacc
      721 ggtatgttca cgaaaccatg tcagaagcgg tttcacctta cggaaagccc ccacctcctg
      781 atgttcctgt gttcccacaa cgacaccact gtgatggtta tcacgagcca gaaagtaaat
      841 atcgagtccc ataacaattc tcctgaataa agccgggact ctccccacag ggatatgtcc
      901 ctgccgggtg atataatgac ggaatacgtg atgacgttat tccggataaa gaaactacgg
      961 cgttctgtta ccgcccaccg gcaagtgccc gcagccagta aagctgatat tcggcctctt
     1021 ccagtgctgc ccagtcatcc ggggataact gtcgcccctg accggacaaa aactgcagca
     1081 gtaagctgtc aaacgcatct ccccttaccg gggtggttaa tacagactcc ggagcagaaa
     1141 aattctcatg ccccaggtac cgttttatcc atttcagcgg cagcgaccag ctggcaccac
     1201 cgacagtgga atgaatacga aaacagtccg gacacagatt gtgcaacagc acaaagactg
     1261 tacgggcaaa gaaatctccc ggctgacgtc cgaaattcag ctcattaaga gccaggatat
     1321 tccggctgtg ctcccgctca atagtgacat tcaccgacca cagcccgtcg gcagaccggc
     1381 agtaaaacgc cagcgtgtcc gttaccggaa acaactctga cgcccgggtc agggagccga
     1441 aatacgcatt ctgcggccgt aacgcctccg gcaactgcca gaaggtttca gtgacagcca
     1501 gaacaaaagc atgccactgc ttttccggta ttgtggaaac ctgctcaagg gtatatacca
     1561 ccggctcacg ggccggacgt gaagataact cagacatgga acattcctca tggttaaaac
     1621 gaaacaacgg ggaatgtctc ccggacggag aactcccccg tcagggtcag gtcatgaatt
     1681 acatcatgtc gtgccgggca aaatattcat ccgctccggc caccatcttt ccggtactga
     1741 tactgtcagc ccagatttca tgattaattt cacgccccag agccatcaga ctgtcgagtg
     1801 cctgacgggc ctgaattccc ggtgcggaaa tctcctcctg ctccaggtca tacaacagcc
     1861 agcccagcgc ctcgccggta tcccacagat acacgtatac cagtgaaggc tccgtctcat
     1921 catccggctc cggggtaata cccagccgtt gccaggcacg taaccgcatc agctcgcctt
     1981 ccatctgact gatatgagcc agcagagcag actgcgtctg aagcgctgaa taatgagcaa
     2041 ttaaaggtga atacatgatg acctccgtct cttaaaggaa gtcctgtccc tgacggaaca
     2101 aaactcccgt cagggaaaac accggcgcaa aaacacggtg cagtaagtaa aagaaaaacc
     2161 gttacgggag caaaacagcc atcattgcaa aatggcagtt gcatcgctca cgatttgaca
     2221 tccttcctgt gactgactgg catcctgccc gtcagtctgc gtcggaaaat tcaatacagg
     2281 cctgcttcag gagcccggct atacgttgat tcagacattc cgtccagcca tcgttccatc
     2341 cacgctccca tggattgcag acatcgttac agccattctt ccatgtcttc catgcctcca
     2401 tctccatgac agcccccagc ctgtggcctt tcacacgtat atgccacaga aacggccgga
     2461 aaagtcctga tttcagttgg tcaccaccgc ttttgtagcc cagatgacgt gcaatacttt
     2521 cagtatattc aaatgcatca ttctgcattc cagccgcctt agccagcgag ctgtacgcat
     2581 cacgaagtat cgtcatacct tccagttgat actcacacca cgcacggaca gccgacactg
     2641 gtgcgattac ccgaagaatg gtgcaggcac gccatacaac aacgtaaagg agtattaaca
     2701 gactatgcat gtgttcacct cagatacaaa aaataacggg aacacacctg ccctgcggga
     2761 agcgtgttcc cgacagggta ataaatgata aatcagcagg gctgtgcgac gattccatca
     2821 ccctgcagac caaaccggta acgcagggtg gtcagttgct ccgccatacg gcaggcagca
     2881 tgccatgtcc tgcgacggga tatccgtccg gtaaaccggt tcagataaat gacctcacgc
     2941 ggtaacggct gataccatcc tgtgtactcg ttgcgataca ttccctgccg gtaatacagg
     3001 cagggtgtcc agacaccgcc acgacggaac atacagaccc gcacgctcag attgccggcc
     3061 ggattagtgt accagcgact gtaatatgac aggttattgc gggaaaaaaa cacccgcaac
     3121 tgagccgtca ttcgccggaa aaaagaagag atgcccggtt cagggcacaa aataaacgtt
     3181 gacataaaga ctcctgtaaa aaaacgggag ccttcgcccc gacggacaaa aactcccgtc
     3241 agggttaaaa caccaccagt acagcatggt gttgctgtca gaaaaagccc gttaataaag
     3301 ggcgcagtta taaa
//
LOCUS       DAZFFI010000230         3305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFFI010000230 DAZFFI010000000
VERSION     DAZFFI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.232"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..1689
                     /locus_tag="ACHJ12_004975"
     CDS             169..1689
                     /locus_tag="ACHJ12_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO4992617.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1714..2052)
                     /gene="maoP"
                     /locus_tag="ACHJ12_004976"
     CDS             complement(1714..2052)
                     /gene="maoP"
                     /locus_tag="ACHJ12_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4992618.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2171..3010
                     /gene="hdfR"
                     /locus_tag="ACHJ12_004977"
     CDS             2171..3010
                     /gene="hdfR"
                     /locus_tag="ACHJ12_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4992619.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3106..3181)
                     /locus_tag="ACHJ12_004978"
     tRNA            complement(3106..3181)
                     /locus_tag="ACHJ12_004978"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3146..3148),aa:Trp,seq:cca)
     gene            complement(3190..3266)
                     /locus_tag="ACHJ12_004979"
     tRNA            complement(3190..3266)
                     /locus_tag="ACHJ12_004979"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3230..3232),aa:Asp,seq:gtc)
BASE COUNT          883 a          813 c          814 g          795 t
ORIGIN      
        1 tcctcgtagt tcatcctttg tccgctgcat ttttgcgagc atcattccac cgccagaaca
       61 actcaacttc acttgcacaa ataaacagga atcagccact aaaattcacg aattagcggg
      121 ctacagcagc gatatcttat gcacataatg gtgactcaag gagggcttat gtcactgtca
      181 attgttcata cccgcgcagc cctgggagta aatgcgcccc cgatcactgt tgaggtacat
      241 atcagtaaag gtctacccgg cttaacgatg gtgggcttac cagaaacaac ggtaaaagaa
      301 gctcgcgatc gcgtgcgcag cgccattatc aatagcggat atgaatatcc ggcgaaaaaa
      361 atcaccatca acctggctcc agctgatctg ccaaaagaag ggggacgata tgatttacct
      421 atcgccattg cgttgctggc ggcctcagaa cagcttacag ccaataagtt agatgaatat
      481 gaattagtcg gagaactggc gcttacaggc gctctgcgtg gcgttcccgg cgcaatctcc
      541 agtgcaactg aagctattaa gtcgggcaga aaaattatcg tcgcgaaaga taacgaagat
      601 gaagtggggc taattaacgg tgaaggatgc ctgatagccg atcatctgca agctgtctgt
      661 gcgtttctgg aaggtaagca cgctctcgaa cgcccgaaac caactgatgc agtatcccgg
      721 gcgctacaac atgatctcag tgatgttgtc ggtcaggaac aaggaaagcg aggactggaa
      781 attaccgccg ctggcgggca caacctttta ctgattgggc cgccgggaac aggtaaaaca
      841 atgctcgcca gccgtattaa tggcctgttg ccagatttaa gcaatgaaga ggcactggag
      901 agcgctgcga tattaagtct ggtaaatgct gaatcagtac aaaaacaatg gcggcagcgc
      961 ccgttccgct cacctcatca cagtgcgtcg ttaactgcga tggtgggcgg tggtgcaatt
     1021 ccagggccag gtgaaatttc gttggcgcat aacggcgtgc tttttcttga tgagttacct
     1081 gaatttgaaa ggcgtacact ggatgccttg cgagagccga ttgaatccgg gcagatccat
     1141 ctttcacgca cgcgagcaaa aataacctat ccagcccgtt tccagcttgt tgcggcgatg
     1201 aatcccagcc ctaccggaca ttatcaggga aaccataacc gctgcacgcc agaacagaca
     1261 ttacgttatc tcaaccggct ctcggggccc tttctcgacc gcttcgatct ctcactggag
     1321 atcccattac caccccccgg cattttgagt aaaacggtag tgccgggaga aagcagcgcc
     1381 accgttaaac aacgcgtaat ggccgccaga gagcgccaat ttaagcggca gaataaactg
     1441 aatgcctggc tggatagtcc ggaaatacgc caattctgca aacttgagag cgaagatgcg
     1501 atgtggttgg aaggaacact gatccatctg gggttatcga ttcgtgcctg gcagcggtta
     1561 ttgaaagttg cacgaaccat tgctgatatt gatcagtctg acattatcac acgtcagcat
     1621 ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc tcatccatct gcaaaaacta
     1681 ctgacataaa aaaagggcat ttcgcccttt ttattaatca tcagaatcgg tgtagtcttc
     1741 agcaccttca acctgcggtt taccgccgga aagggtgtga aaacgttttg gacgcttaat
     1801 acgcgtcata tacttggacc acacgcgttc tgcttctgtc actggctcac gttcgccacg
     1861 gcatactgct acgaagagtt tctcttcctc ggtaaccggc tcgcgtttgc caagatccaa
     1921 ctcattgaag gcataaccat gacgctcaag cagttgtgcc tctttgatgg tgaaatcacc
     1981 atgacgagag aatccacgtg gataatgttt attgtcgaaa tatcgattag tcgtcgtaaa
     2041 gctttccgcc atcctgcacg ctcctaattc tttgaccgag ctagttatgg cgcggagtat
     2101 tagttacgct tgacagagtg taaaacaaaa catttaaatc ataacgacaa ataattttgc
     2161 ggagagcact gtggatacgg aattgttaaa aactttcctg gaagttagcc gaacgcgtca
     2221 ctttggtcga gcggctgaat cgctctatct gacccagtca gcagtgagct ttcgaatcag
     2281 acaactggaa aatcaactgg gtgtgaacct tttcacccgc cacagaaaca atatccgttt
     2341 aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg ctcatgagca cctggcaggc
     2401 cgcccgcaag gaggtggcgc atacctcacg acataacgag ttttctatcg gtgccagcgc
     2461 ctcgttgtgg gaatgtatgc ttaatcagtg gctgggacgc ttgtatcaaa atcaggatgc
     2521 ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg cagtctctgg taaaacagct
     2581 gcatgaacgc cagcttgatc ttcttattac cactgaagcg cctaaaatgg acgaatttag
     2641 tagtcagttg ctgggatatt tcactttagc gctttatacc agtgcccctt caaaactaaa
     2701 gggagatctt aattatctgc gacttgagtg ggggccagat tttcaacagc atgaggcagg
     2761 tttgatcggt gctgacgaag tgcccattct gacaaccagt tctgctgaac tggcacagca
     2821 acagattgcg atgcttaatg gttgcacctg gctacccgtc agctgggcgc gtaaaaaagg
     2881 cggcctgcat accgttgtcg atagcacaac actttcacgg ccgctttatg ccatatggct
     2941 gcaaaatagc gataaaaata cgttgattcg cgatcttttg aaaattaacg tgctggatga
     3001 agtgtattaa tatgaatggc tggcaaggat gccggtagaa ggatttactt cggagagggt
     3061 tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga
     3121 gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc
     3181 taattagggt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
     3241 attctaaccg actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc
     3301 ggctt
//
LOCUS       DAZFFI010000231         3273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFFI010000231 DAZFFI010000000
VERSION     DAZFFI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.233"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..624)
                     /gene="mobB"
                     /locus_tag="ACHJ12_004980"
     CDS             complement(97..624)
                     /gene="mobB"
                     /locus_tag="ACHJ12_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4992620.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(606..1190)
                     /gene="mobA"
                     /locus_tag="ACHJ12_004981"
     CDS             complement(606..1190)
                     /gene="mobA"
                     /locus_tag="ACHJ12_004981"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4992621.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1260..1529
                     /locus_tag="ACHJ12_004982"
     CDS             1260..1529
                     /locus_tag="ACHJ12_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4992622.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1606..2592
                     /gene="srkA"
                     /locus_tag="ACHJ12_004983"
     CDS             1606..2592
                     /gene="srkA"
                     /locus_tag="ACHJ12_004983"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4992623.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2609..3235
                     /gene="dsbA"
                     /locus_tag="ACHJ12_004984"
     CDS             2609..3235
                     /gene="dsbA"
                     /locus_tag="ACHJ12_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4992624.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          828 a          788 c          799 g          858 t
ORIGIN      
        1 aaacgaaagg cccagtcgga agactgggcc tttttgcgct ggtgcagaaa aaggtcggat
       61 gcgacactgg ctgcgtctga tccagcctac tcttgcttat ccgttctgct tttgcatcca
      121 ctccaccaca aaatcagcca gcccctcaac atcattaata tccagtaatg cgacatcaag
      181 attaagcggc acatcactgg ctacagcaat aacatgcctg tctatcacta attcttcagg
      241 tcgatgtccg gctccatcgc gaaacagcac aatctttgcg atctcttcat gcttaaaccc
      301 ttcgaccaga atcaaatcca gctttgaggt atccattcga cttgcgagaa actgtagatc
      361 cagctcttct tcgtctggtg tttccgtcat caaggcccat cgttgctggc tggcaacgat
      421 ggtttgtgcc gcgccagcct tgcgcagctc atagctatct ttgcctggct tatcaacatc
      481 catatcatgg tgcgtatgct taatcagccc tggacggatc cctctggcgc ataatgccgg
      541 gatcagttgt ttcaaaagcg tagtttttcc ggtaccactc cacgcggcaa aggcgagtaa
      601 cggtatcatc gtttttcctg ccatcgggca agctcctctg gcgtattcac gttaacaaat
      661 gcatctttat gatcgctgaa atcaaccgca tgaccgccag ccagacgcat aaatgccatt
      721 acccggcgtt ctcctgcttg cagatattcc agtaataaag gctcaattgc gcggtttacc
      781 agagcaatag tcgggtgatc gcgttcaccg tcgtggaccc acacgacagg cgcatctttg
      841 cgctgatgat taagccgggc tgctaaatcc tcgggaatat aaggagtatc gcacggacaa
      901 aacaaaaacc actcgcctgc ttcctgctgc attactgaaa gcattcctgc cagagggcct
      961 gggtaatccg ccagtgaatc ttcaatcact ttcagaccgc ttgcctgata gatttcctga
     1021 tgacgattag cattaaccac gacgtgagag agctgcgcca taagcgcgtc agcgacatgt
     1081 tgccataatg gtttgccgtt taattccagc aatcctttat ctacgccgcc cattcgtctg
     1141 gctttaccgc ctgccagcac aacgcctgtt atcgtcgtca tcagattcac cgatatcgcc
     1201 tcttttattg tgggattgac cctgctaacg tgtctgtctc aagagtaagg agcattacta
     1261 tgaaatgtaa acgtctgaat gaagttattg aactcctcca gccagcctgg caaaaagagc
     1321 cagaccttaa cctgttgcaa tttttgcaga aactggcgaa agagtcaggt tttgacggcg
     1381 aactggcgga tttgacggat gacattctga tctatcacct gaaaatgcgt gattccgcga
     1441 aagatgcggt gatcccgggt ttgcagaaag attatgaaga agacttcaaa acggcgctgt
     1501 tacgcgctcg cggcgtaatt aaagagtaaa agcttgtaag cggtgccacc aaaatcatcg
     1561 tgaaatgata tccttcatca ttcgtaatgt tttccggatg atgggatgaa caacagcgct
     1621 tttactttcc agacactaca cccggatacc atcatggatg ctctatttga gcaagggatc
     1681 cgggtggatt ccggtcttac cccgcttaac agctatgaaa accgtgtcta tcaatttcag
     1741 gacgaagatc gtcgacgttt tgtcgtcaaa ttttatcgcc ctgaacgttg gacagccgat
     1801 caaatcctcg aagaacatca atttgcgttg cagttggtaa atgatgaagt tccggtcgca
     1861 gcacctgtgg cctttaacgg tcagacttta ttgaatcatc agggatttta tttcgctgtt
     1921 tttccaagcg tcggtggtcg ccagttcgaa gctgataata tcgatcagat ggaagcggtt
     1981 gggcgttatt tagggcgtat gcaccagacg gggcgcaaac agctttttat ccatcgcccg
     2041 accatcggtc tgaatgaata tctcattgag ccacgcaagc tgtttgagga cgctacactg
     2101 atgccttccg ggttgaaagc ggcattcctg aaagcgacag atgagctgat cgccgccgtt
     2161 acagcacact ggcgggaaga cttcaccgtt ctgcggctac atggagactg ccacgccggg
     2221 aatattctct ggcgcgatgg tccaatgttt gttgatctgg atgatgcacg taatggtcca
     2281 gctattcagg atttgtggat gttgctcaat ggcgataaag ccgagcagcg gatgcaactg
     2341 gaaactatta ttgaagctta tgaagaattt agcgagttcg acaccgctga aatcggactg
     2401 attgaacctt tacgcgccat gcgtttggtt tattatcttg cctggctaat gcggcgttgg
     2461 gctgatcccg cgttcccgaa aaatttcccg tggttaaccg gggaagatta ctggctgcga
     2521 caaacggcga cttttataga acaggcaaaa gttctacaag aacccccttt acaattaaca
     2581 cctatgtatt aatcggagag agtagatcat gaaaaagatt tggctggcgc tggctggttt
     2641 agttttagcg tttagcgcat cggcggcgca gtatgaagat ggtaaacagt acactaccct
     2701 ggaaaaaccg gtagctggcg cgccgcaagt gctggagttt ttctctttct tctgcccgca
     2761 ctgctatcag tttgaagaag ttctgcatat ttctgataat gtgaagaaaa aactgccgga
     2821 aggcgtgaag atgactaaat accacgtcaa cttcatgggt ggtgacctgg gcaaagatct
     2881 gactcaggca tgggctgtgg cgatggcgct gggcgtggaa gacaaagtga ctgttccgct
     2941 gtttgaaggc gtacagaaaa cccagaccat tcgttctgct tctgatatcc gcgatgtatt
     3001 tatcaacgca ggtattaaag gtgaagagta cgacgcggcg tggaacagct tcgtggtgaa
     3061 atcccttgtc gctcagcagg aaaaagctgc agctgacgtg caattgcgtg gtgttccggc
     3121 catgtttgtt aacggtaaat atcagctgaa tccgcagggt atggatacca gcaatatgga
     3181 tgtttttgtt cagcagtatg ctgatactgt gaaatatctg tccgagaaaa aataattcat
     3241 tgtgaattac ataaggcccg tgaatattca cgg
//
LOCUS       DAZFFI010000232         3227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFFI010000232 DAZFFI010000000
VERSION     DAZFFI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.234"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..778
                     /gene="yibA"
                     /locus_tag="ACHJ12_004985"
                     /pseudo
     CDS             1..778
                     /gene="yibA"
                     /locus_tag="ACHJ12_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            806..1764
                     /locus_tag="ACHJ12_004986"
                     /pseudo
     CDS             806..1764
                     /locus_tag="ACHJ12_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1776..2237
                     /gene="yibG"
                     /locus_tag="ACHJ12_004987"
     CDS             1776..2237
                     /gene="yibG"
                     /locus_tag="ACHJ12_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4992625.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
     gene            2872..>3227
                     /locus_tag="ACHJ12_004988"
     CDS             2872..>3227
                     /locus_tag="ACHJ12_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO4992626.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAA"
BASE COUNT         1039 a          566 c          717 g          905 t
ORIGIN      
        1 aactaaatga tgatgaatta tttcgcttac ttgatgatca caattccttg aaaaggattt
       61 catctgccag agtattacag ttaagaggtg ggcaagacgc tgttagattg gcaattgagt
      121 tctgctctga taaaaattat atccgtagag atatcggagc atttatactc gggcaaataa
      181 aaatttgcaa aaaatgcgaa gataatgttt ttaatatttt gaacaatatg gcattgaatg
      241 ataagagcgc ttgcgttcga gctacggcaa tcgagtcaac ggctcagcga tgcaagaaaa
      301 acccaattta ttcacctaaa atagtagaac aatctcaaat tactgctttt gataaatcga
      361 ctaatgtcag acgtgctaca gcatttgcta tttctgttat caatgataaa gcaacaattc
      421 cactattgat taatctgtta aaagatccaa atggagatgt cagaaactgg gccgcatttg
      481 caataaatat caataaatat gataatagtg atattaggga ttgttttgtg gagatgcttc
      541 aggataaaaa tgaggaagtc cgtattgaag caataatcgg actttcctac agaaaagata
      601 aaagggtttt atctgtttta tgcgatgagt taaaaaaaat actgtttatg atgatatcat
      661 tgaagctgcg ggtgaattag gtgataaaac gctacttcct gttttagata ctatgttgta
      721 caagtttgat gacaatgaaa ttataacttc cgctattgat aagctgaagc gttcatagcg
      781 tgagttgcct atgcacagtg ggggattccc gccggcacgg tgcagatgct cgaccggctg
      841 gaaagtgaaa tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgtgc
      901 ggcctgaccg tggagcagat gaaaaaccag atggacccgg tgtacacgcc ggcgcgaaaa
      961 atccacctgt accactgcga ccatcgcggc ctgccgctgg cgctcatcag cacggaaggg
     1021 gcaacagcgt ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga tgagaacccg
     1081 catcatctgc agcagctcat tcgcctgccg ggtcagcagt atgatgagga gtccggcctg
     1141 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg
     1201 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt cataaatgta
     1261 gatccgcaag gtttggttga tataaattta taccccgaaa gtgatcttat ccattctgta
     1321 gctgatgaga ttaatatccc aggcgttttc acaatcgggg ggcatggtac ccccacatct
     1381 attgaatccg caacgcgcag tatcatgaca gctaaagatc tagcatatct aattaaattt
     1441 gatgggaatt ataaagatgg gatgacagtt tggttatttt cttgtaatac aggtaaagga
     1501 caaaattcat ttgctagcca attagctaaa gagttacata caaatgtaat aggacctgac
     1561 acgctatgga cgtggtgggg gcgaggaact aatggtaagt taaaaatgga tacagtgcta
     1621 acagcaccaa cgaaccttaa ttcaaataag gatctaatgg ctataacaac aaaagacctt
     1681 ggtaattgga taacatatgg gccatctggg caccccattt ctaatatgca aggtacgcca
     1741 gaaaaaccca gtgatataag ataggttgta gatgtatgaa agcatgcttg ttactatttt
     1801 tttatttttc tcttattggt caattgcatg gtgctgatgt gaaaataaaa gaaaacgaaa
     1861 gtgtgatggg atctacagca atgacctatg atttaagtga agaaaagctg atgaaactga
     1921 agtataaatc acaacatggt gattcagagg catcatttcg actatatcaa tattactgct
     1981 ttactaaaaa taatattgat aaacaattgc gattcttgga aagatcagca tctcagggga
     2041 atgttacagc gcagtttaat tatggggtct ttttatcaga tacaaatcca acattatcag
     2101 aatattataa tttgaataga gctatttatt ggatggaatt cgctgtaaat aacggtaata
     2161 ttgatacaaa gagcaaactt caagagctca aaaagctaaa gcgaatggat agaaggaaga
     2221 ataaggagaa tccgtaaaag caatagttca tcagactgcc gcagcagtag tatgatgatg
     2281 aatcagggct gtactataac tattatcggt aacattaacc gcagcagggg cggtataaca
     2341 ctcaggctcc gattgggctg gaaggtaggt ggaattttta tcagtatccg ttgaacccga
     2401 tttctggaat cgatcccctt gggattagcc acctgtttat acagtataac tttaggtatg
     2461 ctttcgtgtg tttcagatat accatgtgat gataattttt atgatgtgct tagtattcca
     2521 gtcgcttctg gtaataatat gcaatgtaaa aataatcccc gttgtacaca tttataaata
     2581 gatggccaat acctcaaggc ctatggagtt ggaatgttaa tgacccggga gcaagcaata
     2641 gaaaacctga cggcatacga ttagtaccgt cagttaatac aggaacttat aatcgcaatg
     2701 gattttcaat acatagttgt ttaaacgctt ttggtccgtc tttaggacct cgattctgtt
     2761 ccgagggttg tataactggt ttatctaatg acatgcaaaa attaaatgag ttaatatttt
     2821 cagagccaga tagcgcatta acagttacgg actaaaaaat atggcaaggt tattaacaat
     2881 tgtatttttt tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta
     2941 gatttggcag acaaagcaat attatgggca ctaatatcag ccagtaataa agagggacga
     3001 aaagcgtgtt cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca
     3061 tatatggctg cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac
     3121 aaaatagatg ctggactttc cgaatcctat acatgctatt tattaagtaa gggaaagatc
     3181 ataagaccat atctgaaaaa tctaaatcca ctccaacttg ccgctga
//
LOCUS       DAZFFI010000233         3222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFFI010000233 DAZFFI010000000
VERSION     DAZFFI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.235"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..621
                     /locus_tag="ACHJ12_004989"
     CDS             172..621
                     /locus_tag="ACHJ12_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992627.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALDRAKRQTGQQGNQPPAGGDDPYGDDIPF"
     gene            789..1085
                     /locus_tag="ACHJ12_004990"
     CDS             789..1085
                     /locus_tag="ACHJ12_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO4992628.1"
                     /translation="MKPLKTGVIRDVLKDAKERELPIGHGVLRGALISYIHTTRYLNA
                     VIAGGARYDLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            1075..1368
                     /locus_tag="ACHJ12_004991"
     CDS             1075..1368
                     /locus_tag="ACHJ12_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4992629.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWTEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLAEMGINPQDEWRNYSFSDAGDDLALP"
     gene            2194..2433
                     /locus_tag="ACHJ12_004992"
     CDS             2194..2433
                     /locus_tag="ACHJ12_004992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992630.1"
                     /translation="MRKKLRKKVRKSIIKPITKSITQTNRAKPDMTRLAGGKATGKSA
                     QKNAQNETQQPRKTKPIRDDRKSAQIYRIRVSNFA"
     gene            2502..2663
                     /locus_tag="ACHJ12_004993"
     CDS             2502..2663
                     /locus_tag="ACHJ12_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992631.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            3161..>3222
                     /locus_tag="ACHJ12_004994"
     CDS             3161..>3222
                     /locus_tag="ACHJ12_004994"
                     /inference="COORDINATES: protein motif:HMM:NF018262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4992632.1"
                     /translation="MRDIQQVLERWGAWAANNRE"
BASE COUNT          934 a          769 c          893 g          626 t
ORIGIN      
        1 aaaaatctat gagcaatatg cagcaaacag caaaagagcc gcacgggcaa tcactcacca
       61 tttcatgacg ctgtatgacc tgaaagcaga agatgcagaa aaagcggcat gtgatttatt
      121 cgatatgaca atgggtatta tccaggagta ccggaacagg gagcgcagac catgacagca
      181 cagattgcag cttacgggcg gctggtggac gacccgcagg aaaaacagac cagcaagggc
      241 acaccgatga cgctggcgcg tatggcggta tctttgccat gcagtcaggc acaggacgga
      301 caggcgacgt tatggctatc agtcctggca tttggtaagc aggccgacgc gctggcgaga
      361 catgaaaaag gcgaccttat cagcgtggcg ggtaacatgc agatcagtca gtggaccgga
      421 cagaacggag aaatacggca gggctggcag gttatcgctg acagcgtaat cagtgcccgt
      481 tcggcgcgtc ctggcgggaa gcgggggcag caggggaagg cgaccgaagc actggacaga
      541 gcaaaacgac agaccggaca gcagggaaat cagccaccag cgggaggtga tgacccttac
      601 ggtgatgata ttccgttctg agcgacagga gcacaacatg aaccagcaga ccacacaaca
      661 ggaccagaca gtacgtaaac catcacggcg cataccgtgg cagcacatga caaagcggca
      721 gcgaaaaaaa ccacagacgg attaaccgcc tggctgaata ctggcccgag attttcaacg
      781 tggagaatat gaagcccctg aaaactggcg tgatccgtga tgtgcttaag gacgctaagg
      841 aacgggaatt gccgattggt cacggcgtat tgcgcggcgc gttaatatcg tatatccaca
      901 caaccagata cctgaatgct gttatcgctg gtggtgctcg gtatgacctg aacgggcagc
      961 cttgcggcga agtcacggaa gaagataaat cagtcgccag tgaactactg aaacggagac
     1021 tggcgcaaat taagcagcag aacagccagc agcaaacaac cggagacgat ggtgatgata
     1081 gctgacagcg tggcacagaa acttgaggag cgcggtttat ggcgacgtgc ggcgacccga
     1141 tggactgagg ttcttcttca tgcagaaacg gacagggaac gcgaggaagc tgccagacgt
     1201 cgggcgatat gtatcattaa atcccgtcgt atgcctgagc agttcgtcac ctttggcgat
     1261 gtgaaaaagg ccgctgacag aacgctggca gagatgggga ttaatccaca ggatgaatgg
     1321 cgaaattaca gcttcagtga tgcaggcgac gatttagcgc taccgtgata cgcatctcat
     1381 tgccgcattt gctcaattat cgcgattcgc tataatcacc accgaaaagc cgttgatggt
     1441 gttctcctcg ggcaactgga gcccgactga atgaagccat tcaacggttt ttcggcactc
     1501 attccggtgt atctgatccc acacatcggg atgagtgaac gacgaaaaca tcacggtgat
     1561 ttcgtcatgc cgcgataaga atgttcccct ttgggcaact ggagccctct gtgcagaagg
     1621 ttatcgcggt caggttgtga taaaaacgcg cctctttgag caattgaagc tcactgtaaa
     1681 gcggcttatc acagcaggct gtagcgggtg ctcatctctg ggcaactgaa gccctctgta
     1741 cagaggcccg ttacggctga ccctgaatga tttcgccccg tgcaattgaa gcacccagcg
     1801 gagttattca gggtttttta ttgcccgtcc accagcacca catctgtagc attgatgcgt
     1861 atacattcgc cgtcaattgc accattccgg ccctcaccga tccgcagcga gagcatatag
     1921 ccagacggcg ggaaatatgc cttgcgaact tcaggatgct gtaaataaga ctgttgacga
     1981 tatgggaatc agacgggatc ggcgacatta cgggttatgg aaataacagc caggtaaatt
     2041 ggtgaatata cggcaggctg caatcagagc cgaacatagc ggcctccgtc accattatca
     2101 gacagtagcg gtagtccttc catcacagca aaatgctacc cacttcacag ccccttggaa
     2161 gcctcttcga tggggtgaaa aatccctgaa aaaatgcgca aaaaactgcg caaaaaagtg
     2221 cgcaaatcga tcataaaacc gatcacaaaa tcgatcacac agacaaacag agcaaagcca
     2281 gatatgacgc ggcttgcagg gggaaaggcc accggaaaaa gtgcgcaaaa aaatgcgcaa
     2341 aacgaaacgc aacagccgcg caaaacgaaa ccgatacgcg atgatcgaaa aagcgcgcaa
     2401 atttaccgga ttcgggtaag caattttgcc tgatttgggc aaaatacggt gacgccgtaa
     2461 cagtgacatt gacaccaaaa cagtgacaca caggagggcc gatgaccgaa cgttcaccaa
     2521 cagagataat tcacgggatt attgaagagc aggaggaacg cgagttaatc ctcatgattg
     2581 aagagcagag agaacgccag cgcaaacagg aggccacgca gccggacagc gtgagcgctg
     2641 aaaatcaggc tgatgtgacg tgatggtgtc gaactgagta gctttaagcg actctgtgac
     2701 tggttttaaa cgagtcgcaa aaagagccgg agaaaaacca cgaatctggg gttttttcta
     2761 agcacgctgt acacggtgga acatatggtg gtcaggagac ggcaggagca acgccagaac
     2821 gcgcccccgt accagtggca taagccggag cggttacggc ggtaacttgt tgattaaaag
     2881 gcggctcaat tctgagccgg gtagctgacg gcgttaactg gctgatattg ttacaaagct
     2941 catttttgag ctttggccta acctactgat attttcggaa acctcaattt gaggagaccg
     3001 caaacgcaaa attgcgctgg ctacattcct cacttctgag gactgttaac aagctggctg
     3061 aaagacagtc acaagctacg aaattttcgt agtttaactt tcatccagtg catccctgaa
     3121 ttacgggtat gtaaccacag ctattacgag gtgattgttg atgcgtgata ttcagcaggt
     3181 tctggaacgc tggggagcgt gggcggcaaa taatcgcgag aa
//
LOCUS       DAZFFI010000234         3191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFFI010000234 DAZFFI010000000
VERSION     DAZFFI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.236"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..585
                     /locus_tag="ACHJ12_004995"
     CDS             <1..585
                     /locus_tag="ACHJ12_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992633.1"
                     /translation="EFVAWLKEKGAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGC
                     AIEVMGTYNAAIVRVTIGEYGGDGFLNGTVCTCTVYGDTQRFHWRMDYSTKNKPTTAS
                     LTVNGWERDEVTGRLRQWGSIEVSEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPN
                     AIKMYTLSNPSLVSVTLITVRGCYGKFSWEAIGY"
     gene            595..819
                     /locus_tag="ACHJ12_004996"
     CDS             595..819
                     /locus_tag="ACHJ12_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992634.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            819..1490
                     /locus_tag="ACHJ12_004997"
     CDS             819..1490
                     /locus_tag="ACHJ12_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO4992635.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKSGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            1865..3016
                     /locus_tag="ACHJ12_004998"
     CDS             1865..3016
                     /locus_tag="ACHJ12_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4992636.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDAAENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKKIATSVGSWSAARGQQSVALGGGTYAYADASTA
                     LGSIAFVDNTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT          836 a          630 c          957 g          768 t
ORIGIN      
        1 gagtttgtcg cctggctgaa agaaaaaggg gcatttgacc agccttactg gatgatgaag
       61 gcatcactgc ttgcggaatt taataaggtc atcacggatg tcggaccggg aaaactcaat
      121 ctgggaggct gcgccattga ggtgatgggg acgtataacg cagccatagt ccgggtcacc
      181 atcggcgaat acggtggcga tggttttctg aacggcacgg tctgtacctg tacagtttat
      241 ggagacacac aacgttttca ctggcggatg gattacagca caaaaaacaa gcccacaaca
      301 gcaagcctga cagtaaatgg ctgggagcgt gatgaagtaa ccggtcgttt gcggcaatgg
      361 ggcagtattg aggtaagtga ggatgatggt aagcttatga cacagcactc tatccgtttt
      421 cctgtcgctt ttccggttgc tgctctgaat gctcaggtgt ctgcagtggg agaccccaac
      481 gccattaaga tgtataccct gtcgaatccg tcattagtgt ctgtgactct gataacagtc
      541 aggggatgct acggcaagtt ttcatgggaa gcgatcggat actgagggca ttgtatggaa
      601 cagtatgttt tttcaccgtc agagaatatg ttctatcccc tgtccctgcg gccggtttat
      661 gaggcagcag ggcgctggcc ggaagacggt attgtggtgg attatgtggt gtataaagtc
      721 tttgcagcgg atgctgcacc ggcaggtatg aagcggggcg tcggtgcgga aaaaatgccg
      781 gtctgggtgc cggtatcgga ggagggcacg gaaacatgat gcatatcaga aatttttcgt
      841 attacacccc ggcggagccg gatgttgcag gcgcgatgta cctgaagtct gaggatggtc
      901 aggactggta tgagtgtcag gcgttgtttt cgccggagac gctgaaggtg gtgtatgaca
      961 gccggggtgt gattacggga tacggtaagg acactgcgtt actgtggccg gtgaaccaga
     1021 gtgtggcaga agtgccggat accccggaga gccggaaaat tgacctgagc gggcgctggg
     1081 gttttgacgg ggagaaaatc acggacctgc tgaccgcgga gaaagcgcga gggatgaagg
     1141 gcgatgaaat taacgcctgg cgtaatgcga tggaagcggc gaactacacg tttgagcaca
     1201 atgggcgtaa atgggactac gggaagtcaa cgcagacgcg tcttgagccg tcggtggcgg
     1261 cagcgaaatc ggggaaactg ccggaggcgt ttttctggac ggatgcggaa aacaatgatg
     1321 tgccggtgac agcagaagag cttattgcgc tgagtgaagc ggcagagcag gcgatgttca
     1381 ccaaagggat ggaaatccac gttcgccagc gcaccatgaa gaaagagctg gaaaagctga
     1441 ccagtgcgga tgagatactg gcatacaggg ttggctgggc acaggagtaa ttggtggaaa
     1501 agagggattg tccctttctg tttttactga aagagtcgta ataattacgc agggaaatat
     1561 catggctgaa ctaaacagtt aaattttgat gatattgtga tgttgctcag aaattacagt
     1621 gtaagcagat gtgaaatatc cagcggcatg agatcagttt tttatttagt attgctctgc
     1681 cttgtgctcc agtatttgga gtaaaggtgt aaaaaataca ctaagatatt tcctaaaaat
     1741 taaaattaaa tttgtggggt tttttaggaa tatcctcatg ggtgccgaaa tacatcagat
     1801 tttctttatt catccgatgt atttcggttt gtttgtatac aaataaattc atgaggaatc
     1861 atacatggat aagtctttat cacgaagtgt tatttccgtt gttattggcg gtattttatt
     1921 tggtgtaagc gctggcgttc tggctcagga tgcagcggag aataagctta tcgcctcagg
     1981 gagtatccca gaaaatacag atctttctgc ttcagctcgc gcggatggag agattgcgct
     2041 agctgttggt acaggggcca gcgcggataa aaaaattgca acatcagtgg gatcatggtc
     2101 tgcagcacga ggacagcaaa gtgtggcttt aggtggggga acatatgctt atgcagatgc
     2161 atctacagcc ctgggaagca ttgcatttgt tgataacacg gctacatatg gcacggcagc
     2221 aggtaaccga gccaaagttg ataaagatgc aacagaaggt actgcgttag gggcaaaggc
     2281 aacagtaaca aataaaaaca gtgttgcatt aggagctaat tcacgcacaa cccgtgacaa
     2341 tgaggtttat atcggttatg aagcagagcc gggtaaggct tataaaaccc gggtgcttgg
     2401 tggtctgagt gacggtactc gtccctcaga tgctgcaacc gttcgtcagg ttgaccgtgt
     2461 aaaagacagt gttgagcaac tggctcagga tacgaatacc cgccttgtgg ttgaagcgaa
     2521 aaagtctcgt gaatatacag actccagaac gacagttggt gttaactccg acggaaccct
     2581 gacccgtgca gaaggagcca gcaaaaccct ggctgttaat gatgggcttg tagctttgtc
     2641 cggcagaaca gaccgtattg atgctactgt tggtagtgtt gaccgccggg ttactaaaaa
     2701 tacacaggcg attcagtcca cttcccgtca gttgcaggag cacaacacac gtctgaacag
     2761 tcatcagcgt cagattaatg aaaaccacga agagatgaaa cgtgcggcag cccagagcgc
     2821 agcgctggca ggtcttttcc agccgtacag tgtgggtaaa ttcaacgcca ctgctgccgt
     2881 tggtggttac agtgacaaac aggctgttgc cgtgggtgtt ggttaccgtt tcaatgagca
     2941 gacagcagcg aaagcaggtg tggcagcgag cgatggcgat gtatcttgga acgctggtgt
     3001 gaactttgag ttttaattgc tgacagtgac atgaagcatg acggcaggcg tggagttttg
     3061 tctgccgttt tttctcatgt gagcatgaaa caggtggcag cgatgaaagt gattctggcg
     3121 acccgcaacc gttatctgga gtatggcctg cagcagatgc ttgaggggta caggataata
     3181 ctggcgaggg a
//
LOCUS       DAZFFI010000235         3190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFFI010000235 DAZFFI010000000
VERSION     DAZFFI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.237"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2429
                     /locus_tag="ACHJ12_004999"
     CDS             <1..2429
                     /locus_tag="ACHJ12_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4992637.1"
                     /translation="GCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNAL
                     INNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYPIEKEK
                     QITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQG
                     DNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAK
                     LTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNR
                     IIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYT
                     TAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNS
                     LGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNE
                     YINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSFSLSRD
                     FDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTA
                     SWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLN
                     ASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVS
                     SYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSH
                     PPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTG
                     TVILPCISQN"
     gene            2445..>3190
                     /locus_tag="ACHJ12_005000"
     CDS             2445..>3190
                     /locus_tag="ACHJ12_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4992638.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVISYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRD"
BASE COUNT          990 a          673 c          647 g          880 t
ORIGIN      
        1 ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc
       61 gtgaaaacgt taacctttcc caatttgaaa ctgatggcca attacccgta ggtaaatact
      121 cattaaacgc tcttattaat aataaaagga cgccaatcca tctggacctg caatgggtat
      181 taattgataa ccaaactgca gtttgtctga ctccagagca attaacatta ttaggcttta
      241 ctgatgagat tattgaagtg gctcagcaaa acctgatcga tggttgttac cctatcgaaa
      301 aagaaaaaca aattacaact tatctcgata aagggaaaat gcaattatcc atatctgcac
      361 ctcaggcatg gttaaaatac aaagatgcaa actggacgcc tcctgaactt tgggatcatg
      421 gtattgctgg ggcatttctt gactacaatt tatatgcctc tcattatgca ccacatcagg
      481 gcgataattc acaaaatata agttcctatg ggcaggctgg ggttaatctt ggggcctggc
      541 gcctgcgtac tgattaccag tacgatcaat catttaacaa tggcaaaagc caggcgaaca
      601 acctggattt tccgcgtatt tatttgtttc gcccaatccc agcaattaat gcaaaactaa
      661 ctataggtca atacgatact gaatcctcta ttttcgactc tttccatttt tctggcgttt
      721 cgttgaaaag cgatgagaat atgttgccgc ctgatctacg tggttacgca ccgcaaatca
      781 cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt attatttatc
      841 aagaaaatgt tcctcctggc ccatttgcta ttactaattt attcaataca ttacaggggc
      901 aactggacgt caaggttgaa gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta
      961 atagtattcc ttatctgacg cgtaaagggc agattcgcta caccactgct atgggtaaac
     1021 cgaccagcgt tggtggtgat tccttacaac aacccttctt ctggactggt gaattctcat
     1081 ggggttggct gaacaatgta tccctgtatg gtggttcagt tttaacaaac cgtgattatc
     1141 aatctctggc tgccggcgtt ggctttaacc ttaactcatt gggttcatta tcttttgatg
     1201 tcacacgatc tgatgctcag ttgcataatc aggataaaga aacgggttat agctaccgcg
     1261 ctaactattc aaaacgtttt gaatctaccg gtagccagct cactttcgct ggttaccgtt
     1321 tctctgataa aaactttgtg acaatgaatg aatatatcaa tgacactaac cattacacga
     1381 attatcagaa tgaaaaagag agttatattg tcacgtttaa ccagtatctt gaatcattaa
     1441 ggttaaatac atacgtaagt ttggctcgta atacttactg ggacgccagc agtaatgtga
     1501 attattcatt ttcacttagc cgcgattttg atatcgggcc attaaaaaac gtctccactt
     1561 ctctaacatt tagccgaata aactgggaag aagacaacca ggatcaactg tacctaaata
     1621 tttcgattcc ctggggaacc agtagaacat tgagctatgg tatgcaacga aatcaggata
     1681 acaagatttc gcatactgct tcgtggtatg actcttccga tcgaaataat tcctggagcg
     1741 tttctgcttc aggcgacaat gatgaattca aagatatgaa agcgtcacta cgcgccagtt
     1801 atcagcataa taccgagaac ggtcgtctct atctctccgg gacatcacag cgagacagtt
     1861 attattctct gaatgccagt tggaatggtt cattcacggc gactcgtcac ggagccgctt
     1921 tccatgacta tagtggtagt gctgactcgc gttttatgat cgacgcagat ggtgcagaag
     1981 atattccgtt gaacaataaa cgcgcggtaa ctaatcggta tggcatcgga gttattccat
     2041 cagtcagcag ttacataaca acatcattaa gcgttgatac ccgaaatctt ccagaaaatg
     2101 tggatatcga aaactcggtt atcaccacca ccttaaccga aggtgctatt ggctacgcca
     2161 aacttgatac ccgcaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
     2221 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
     2281 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
     2341 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
     2401 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     2461 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     2521 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     2581 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacta
     2641 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     2701 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     2761 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     2821 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     2881 tagaagatga tggggtaatt tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     2941 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     3001 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     3061 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     3121 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     3181 aacgagataa
//
LOCUS       DAZFFI010000236         3163 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFFI010000236 DAZFFI010000000
VERSION     DAZFFI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.238"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..556
                     /locus_tag="ACHJ12_005001"
     CDS             <1..556
                     /locus_tag="ACHJ12_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4992639.1"
                     /translation="FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLG
                     SVFMQLELGDKYRSQFFTPWSVASMMAQMQMGNVKALFENKPFITLSEPACGAGSMIL
                     AMADTLNRSGYPAYHRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRV
                     LLTPGHYLGNWDHRLRHLQEQNVA"
     gene            complement(790..1342)
                     /locus_tag="ACHJ12_005002"
                     /pseudo
     CDS             complement(790..1342)
                     /locus_tag="ACHJ12_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1459..2364
                     /locus_tag="ACHJ12_005003"
     CDS             1459..2364
                     /locus_tag="ACHJ12_005003"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4992640.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            2409..>3163
                     /locus_tag="ACHJ12_005004"
     CDS             2409..>3163
                     /locus_tag="ACHJ12_005004"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4992641.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQ"
BASE COUNT          877 a          556 c          821 g          909 t
ORIGIN      
        1 gttcagtgag gcccgggaac agaagtatct gcggatcgtc agtggttatg aaaagcagga
       61 tgttatccac attgcgcatt tgcttgctca tgtcattgag ggactggagc agggattctg
      121 tgattttctg ggcagtgtgt tcatgcaact ggaactgggg gataaatacc gtagtcagtt
      181 tttcacgcca tggagtgtgg ccagcatgat ggcacagatg cagatgggga atgtgaaggc
      241 gttgtttgag aataaaccct ttatcacgct cagtgaacct gcctgtggtg ccggcagtat
      301 gattctggca atggctgaca ccctgaaccg ttcggggtat ccggcatacc acaggatgtg
      361 ggtgtcggca acggatattg acccgctggc agccggcatg gcgtatatac aactgtcgct
      421 gtgtggtgtg gccggagaag tggtgattgg taactcgctc tgtaatgagc gacgccgcgt
      481 actgctgact cccgggcatt acctgggaaa ctgggaccat cgtctgcgtc atcttcagga
      541 gcagaatgtg gcatgatgca gaatgtttta tcactgcgcc agtcttcgga ctggcgtttt
      601 gttcaactgc cagttccctg aatgtttgat ttgttcaata aagcctgttt atcaataaat
      661 tttctgacga cagggaatgg tatgttttta ttagttttta tgtgttggac aacaagacag
      721 aataatgatg tctgttttat gtatacgata tagccactga ttcaacgcat tatcggctat
      781 attgccagtc ttatttagtg ccagattcat tgtatcgaag gccgctgccg tcagtagatg
      841 gctggtcagt tctatcagac agaccatttt tacctgagga taaagtgtct ggtttccacc
      901 atgtatttgg cgagtgaagc tttatcgtgt tcttgcgtgt cctgtgttcg tcagaacacg
      961 ccatcaatgg tcagcagatt cagatcgcac cagagcggat atggcgtggt gttatgccag
     1021 agctgcgctg tttgagtgaa cacgcagcgt atggctccgc ttcctggccg ctggcgggca
     1081 tggagcacgg cgctactgcc tggcagtatg atgtccagcc ggttcacaat ctgatgaagg
     1141 ttccttaagc tcatgggcca tactgataat tcaccagacc atcatttcca gaggaagacg
     1201 ccgtttgtgt agcgtgacag tgtcggactc ttcagggctg tgggtaatca gttccagggc
     1261 aagatagtcg cagagggtta cgcagagaat agtaactgga cactggatcg atcaaccgat
     1321 ccttaactga gcggcatgac agctttctga tggatttttt tgataatctt cacattgtgc
     1381 ttcttttctg ttggataatt tttgcacttg gtggtgtact aggtatccca ttatgcagtt
     1441 aagtgaaggt gattaaaaat gcaatgtgaa tttaaagggg tcatatcagc attacccact
     1501 ccttatgacc aatcccagca aatcgatatg gagagtcttc gaaaactgat ccgttttaat
     1561 atcgaacaaa atattaaggg attgtatgtt ggaggctcta ctggagaagc atttcttcag
     1621 aatgttgcag agcgagaaaa aattcttgaa acagtagccg atgaatcaga tggaagactg
     1681 acacttatag cgcatgtggg cggaatcagt acggcagaaa gcgaggtact tgcaaaagct
     1741 gcaaaaaaat acggatatca tgctatatct gctgttactc cattttatta tcctttttca
     1801 tttgaagagc attgtatcca ttacaggaag attattgatt ctgcagatgg acttccgatg
     1861 gtagtttata atataccggc tcttagcgga gtgcgttttt ctttagatca gataaatgag
     1921 cttgtcacaa tacccggggt ttgtgcatta aaacagacat ccggtgattt attccagatg
     1981 gaacaaatca aaaggaatca tccagagctt gtgttatata atggttacga tgaaatattt
     2041 gcatcagggt taattgcagg tgcagatggg ggaatcggta gtacatataa tataatggga
     2101 tggcgttatc ttgaaatatt tgaagcagtc aaaaacaatg atgtgattaa ggcgaaggaa
     2161 atgcaggtgg cctgtaacca ggttattgac actcttattc agagtggtgt cctggcaggt
     2221 ataaaaactc ttctttacta tatggggatt ctaaatactc ctgtatgcag aagtcctttt
     2281 tctccggtaa aggaaaagaa tctggatgta ttatcaaaac ttgcagagcg actgctcgaa
     2341 gaacatgaca gaaataaaaa atgaaaataa tctgaataat tgcaagattg ttatataggt
     2401 gaaaatgaat gataacactg gctgttgata taggaggaac gaaaatttca gcggcgttga
     2461 tatctgatga cggttcattt ttgctgaaaa aacagatatc aacacctcat gaaagatgtc
     2521 ctgacgaaat gacaggggct ttacgtctac tggtttctga aatgaaggga acagcggagc
     2581 gatttgcagt agcatcaaca gggattatta ataatggagt attgacagct ctgaatccag
     2641 acaatcttgg agggctgaaa gagtatcctc tgaaaaatat aatggaagat ataactggtt
     2701 taaatggaag tgtgataaat gatgcccagg cagccgcatg ggcagaatat acagttttgc
     2761 caaaagaaat ttgtgatatg gtttttataa ccgtttctac gggggttgga gggggaattg
     2821 tagttaacag gaaattatta acaggtgttt ctggattagc cggacatgtt gggcatatat
     2881 tgtccggagt aacggataca gaatgtggat gtggtcgtcg gggatgcgtt gaagcggttt
     2941 catctggtcg ggccatcatg ggggcagcaa aaaatgaact tgcaggatat agcaccaaat
     3001 atatttttga attagcgaga cagggatata aggaagcaga atttttgaca gagcgttctg
     3061 ccagtactat tgctgaatta atagtaagtt taaaattact gcttgattgt caggttgttg
     3121 ttgtcggagg aagtgttgga cttgctgatg ggtatattca gaa
//
LOCUS       DAZFFI010000237         3155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFFI010000237 DAZFFI010000000
VERSION     DAZFFI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.239"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..925)
                     /locus_tag="ACHJ12_005005"
     CDS             complement(<1..925)
                     /locus_tag="ACHJ12_005005"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4992642.1"
                     /translation="MSELSYLEKLLDGVEVEWLPLAKVCRLINGRAYKQEELLSKGKY
                     PVLRVGNFFTNQNWYYSDLELDQDKYCDNGDLLYAWSASFGPRIWHGGKSIYHYHIWK
                     VVPDSNLICKQFLYYLLQWDTKALKDAHSTGSTMMHISKTTIEKRLVPIPCPDNPEKS
                     LAIQSEIVRILDKFTALTAELTAELNMRKKQYNYYRDQLFSFNTEDVPHLPMGQKDIG
                     EFIRGGTFQKKDFIDAGVGCIHYGQIYTYYGTYTEKTKTYISTALAKKCKKAQKGDLI
                     IATTSENDEDVCKAVAWLGSEDIAVSSDACIY"
     gene            complement(922..2478)
                     /locus_tag="ACHJ12_005006"
     CDS             complement(922..2478)
                     /locus_tag="ACHJ12_005006"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO4992643.1"
                     /translation="MTSLQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTNVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGYEVQK"
     gene            2785..2952
                     /locus_tag="ACHJ12_005007"
                     /pseudo
     CDS             2785..2952
                     /locus_tag="ACHJ12_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            3020..>3155
                     /locus_tag="ACHJ12_005008"
     CDS             3020..>3155
                     /locus_tag="ACHJ12_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4992644.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
BASE COUNT          854 a          716 c          658 g          927 t
ORIGIN      
        1 tgtaaataca ggcatcactg cttactgcta tatcttcact gcctaaccat gcaacagctt
       61 tacatacatc ctcatcattc tcactagtag ttgcgataat caaatctccc ttttgagcct
      121 tcttgcattt ttttgcaagt gcggttgaaa tatatgtttt tgttttttct gtatatgttc
      181 cgtaatacgt atagatttga ccatagtgta tgcaccctac acctgcatct ataaagtctt
      241 ttttctgaaa agtaccaccc cggataaact cgccaatatc tttttgcccc ataggtaaat
      301 gtggaacatc ttcggtatta aaactaaaca actggtcgcg atagtagttg tactgttttt
      361 tacgcatgtt aagctcagcg gtaagctcag cggtaagtgc agtaaactta tccagaatcc
      421 gaacaatttc agactggatg gcaagggatt tctccggatt atccgggcag gggatgggga
      481 ctaacctttt ttcaattgta gttttgctga tgtgcatcat agtagaacca gtgctatgtg
      541 catctttaag tgctttagta tcccattgta acaagtaata tagaaactgt ttacaaataa
      601 gattcgaatc tggaaccact ttccatatgt gataatggta aatagattta cccccatgcc
      661 aaattcttgg accaaatgat gcggaccaag catataaaag gtcaccgtta tcacaatact
      721 tgtcttgatc taattctaaa tctgagtaat accaattttg gttagtaaaa aaattaccga
      781 ctcttaatac tggatatttg cctttggaaa gcagttcttc ttgcttatat gctctcccgt
      841 ttattaaacg acaaacttta gccaatggca accactcaac ttcaacccca tccagcaatt
      901 tttccagata acttaactcg ctcatttctg cacctcgtag ccttcaattt cagccacaat
      961 cgcatcaata tctttgcgca attggtcgat tttgctgacc gtggttttca gctctgcatt
     1021 caactcagcg atatcgataa tttcgcgagt atctttcgct tccacatagc tgctcaccga
     1081 caggttatag tcattcgcga caacggtctc aaacgcgaca gatttcgcca gatgagcaac
     1141 atcttccttg ctggcaaata cctgcataat ctgttcgata tgggcatcgg tcagaatgtt
     1201 gttgttagtc tcttttttga acagctcgct ggcatcaata aactgaacgt tggtatccgt
     1261 tttatgttta gacaacacca gaatattgac ggcaatggtg gtgccaaaga acaggttcgg
     1321 tgcgagtgaa atcacggttt cgacatagtt attgtcaacc agatactgac ggattttctg
     1381 ctccgcgccg ccacggtaaa aaatgcccgg gaagcagaca atcgcagcac gacctttggc
     1441 agaaagatag ttcagcgcat gtaatacaaa cgcaaagtca gctttggatt tgggggccag
     1501 aacgccagcc ggggcaaaac gttcatcgtt aatcagcgtc gggtcatcgc tgccaatcca
     1561 tttcaccgaa tacggcgggt tagaaacgat ggcatcaaac ggtttttcat ctctgaagtg
     1621 cggctcagtc agcgtattgc ccagcttgat atcaaacttg tcgtagttga tgttgtgcaa
     1681 aaacatgttc atacgcgcca ggttataggt cgtatggttg atttcctgac caaaaaaacc
     1741 ttcttcgatg atatggtcat caaactgttt tttagcctgc aacaacagcg aaccggaacc
     1801 tgctgccggg tcgtagattt tgttaacgtg ggtctggccg tgcattgcca gttgtgcaat
     1861 cagcttagag acgtgctgcg gtgtaaagaa ctcgccgcct gacttaccag cattcgccgc
     1921 atagttagaa atcaggaact cataggcatc gccgaacagg tcaatctgat gttcgttaaa
     1981 gtcgccaagc tttaaccctt caaccccttt cagaaccgca gccaggcggg catttttatc
     2041 cttaacggtg ttacccaggc ggttactggt ggtatcgaaa tcagcaaaca aacctttgat
     2101 gtcagcttct gaagggtaac cgtaagcaga actttcgata gcaacgaaga tactgtttaa
     2161 atctgcattc agtctgtcat tggtatttgc tttcgcagct acgttgcaga aaagctgact
     2221 tgggtagatg aagtagcctt tagttttgat ggcatcgtct ttaatgtcat cagtaattac
     2281 gctgtcatcc agtttcgcat aacagatact gtcatcaccg gcttcaatat agctggaaaa
     2341 attttcgctg ataaaacggt agaaaagcgc gcccagaacg tattgcttaa agtcccatcc
     2401 atcgaccgaa cctctgacat cgttagcaat ttgccagatt tggcgatgaa gctccgcgcg
     2461 ctgctgaagg ctggtcattt tttatccttt gaatgtgttt ttaacgtcga cttttgccgg
     2521 gattttaccg gaagtttttt ggtttttcag atttgtatga cggtgaggag gggattcagt
     2581 accagattgt tagtgcatct tattcagcat tcaccgtttg agtcaggagg ctactttata
     2641 tgcccatcgc ccatccgtag gtgatcagat aatttattgc gagtcacctt cccgattttc
     2701 aatctcatta tttacccaat ctttcaccag tggataatcc gtttgctact aacggcgagt
     2761 gtattttttt acggatgaaa gattatgcaa acagaagctg aagtcttaac cgatcataac
     2821 gaactaattt gctcgaccag tatcgagcgt atcgtcacag gacgtgatag tgcactgaac
     2881 cagatagcga cgttaatcca aaaacttcat gatatctcca gtctgacatc cagtattggc
     2941 ggtgatgtgg ctgctgatct tacccagcaa tagtggacac gcggctaagt gagtaaactc
     3001 tcagtcagag gtgactcaca tgacaaaaac agtatcaacc agtaaaaaac cccgtaaaca
     3061 gcattcgcct gaatttcgca gtgaagccct gaagcttgct gaacgcatcg gtgttactgc
     3121 cgcagcccgt gaactcagcc tgtatgaatc acagc
//
LOCUS       DAZFFI010000238         3153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFFI010000238 DAZFFI010000000
VERSION     DAZFFI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.240"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..362
                     /locus_tag="ACHJ12_005009"
     CDS             <1..362
                     /locus_tag="ACHJ12_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4992645.1"
                     /translation="ELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLRDA
                     GQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPDPT
                     LSEVSGGSGRADDIIGL"
     gene            652..1215
                     /locus_tag="ACHJ12_005010"
     CDS             652..1215
                     /locus_tag="ACHJ12_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4992646.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPLPVVIS
                     PGIEYMDDGLPDPVKAMGRLLVENINTDPRLALDVAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGSG"
     gene            1212..2873
                     /locus_tag="ACHJ12_005011"
     CDS             1212..2873
                     /locus_tag="ACHJ12_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4992647.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEDTFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLISLNPEPKKKEYAVFFI"
     gene            2937..>3153
                     /locus_tag="ACHJ12_005012"
     CDS             2937..>3153
                     /locus_tag="ACHJ12_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992648.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEG"
BASE COUNT          704 a          772 c         1005 g          672 t
ORIGIN      
        1 ctgagcttcg tacactccag agcagaatca aaacactgaa tacccgacgg gtgaatattc
       61 tgaagggtga acagcgtcgt gtcagtggca gtgcacgtgt ttccctcaag cgtcatatct
      121 ggctcaggga tgccgggcag tgctgtctct gtggtcgtgt ggttgacctc tgtgacagtg
      181 aactcgatca ccgaattgca cttcagttcg gtggtggtaa tgaggagacg aatctctgga
      241 cgctctgtac cgaatgccat cgacaaaagt ctgctcgtga agcggcgggt ggtatgccgg
      301 acccgacact gtcggaggtg tccggaggta gtggcagagc ggacgacatc atcggactgt
      361 aacccgaccc gggggggtat catccggcgt aaaaaacgat cgctccggac accgcgcccc
      421 ctctcacgca gagaaaaaat tcccgtttca ggtcagttaa catgttaact ggcttcacgg
      481 gcatttttgc agtttttatc tttattattc agtttgttgc gcggaaaaaa tgttaacagg
      541 ctttttcagc aaatgttaac caggcagcag ttaacatttg cggcatgaga cgccgggaaa
      601 aatgggctga accatacccg gctgagtgca ttctggaccc gggaggaggc tgtgctgaca
      661 acgcaaaaac gaaaatttgc gctggcgctc atgtccggga aaaacaaaac agcgtcagcc
      721 attgccgccg gttattcggc gaagacagcc agggttaaag gctcgcagct ggcaaaagat
      781 ccggaggtgc tcgcgtttat agcccgtaag cagtgcgaga cggtagaggt ggatgaggtt
      841 cctgtttacc ggcagaaaaa atcagagcag gaggataaac cccgtcgccg tgaggcggct
      901 gcaataccac agccggacga aacaaatccg gagatgccac tgcccgtggt gatatctcct
      961 ggtattgagt atatggatga cggtcttccc gatccggtga aagcgatggg gcgtcttctg
     1021 gtggagaaca ttaataccga ccccaggctg gcgctggatg tggcttataa gctggcacag
     1081 ttcacgcacc acaaaaaagg ggatgccggt aaaaaatcgg caaaaggtga cgcggcgaaa
     1141 aaagcggcta accgttttgc ggtgccacca cccccccgac tggtggtgaa taatgataat
     1201 gagggcagcg gatgatacct gtgtggagca cggcctgccc ggactgggca gagcgcctga
     1261 aaaaggggct gtcgattatt ccggctccga tttacccgga ccaggccgca catgccctgg
     1321 cgatttttaa acaactgcgg attgtggatg caccgggcag cccgacgttc ggggagtcct
     1381 gtgcaccgtg ggtgtttgat ctggtggctg ccctgtttgg ctcctacgat gcgcagaccg
     1441 gtgtacgcca tataaaggaa gtgttcattc tgatacccaa aaaaaacagc aagtccacgc
     1501 tggctgccgg gatcatgatg acggcgctgt tactgaactg gcggcaggcg gcgggctaca
     1561 ccattctggc cccgaccgtg gaggtggcgg ctaatgcctt caatcctgcc agggatatgg
     1621 tacgacggga cgatgatctg gatgacctct gtcaggtgca gacacatatc cggaccatca
     1681 cccatcgggt gacggacacc accctgaagg tggtggcagc cgatccgaat acggtgtccg
     1741 gtatcaagtc cgtggggaca ctgattgatg aactgtggct gtttggcaag cagtacaaag
     1801 cggaggacat gttacgtgaa gccataggtg gccttgcctc ccgcccggaa gggtttgtgg
     1861 tgtatacgac cacccagtcg aatgaaccgc ccgccggggt gttcagacag aaactgcagt
     1921 acgcccggga tgtgcgcgac ggcaaaattc atgatccgca ctttctgccg gtgatatttg
     1981 agcatccgcc ggagatggtg gagcgcggtg agcacctgct gatggaaaac ctcgccatgg
     2041 tcaatccgaa tctcggttat tcggtggatg aggcctttct gtaccgggag tatcgtaaag
     2101 cccgggaagc cggtgaagat acattccggg ggttcatgtc aaaacacgcc aatgtggaaa
     2161 ttggtcttgc cctgcgttct gaccgctggg cgggtgcgga tttctgggag cagcagggca
     2221 ggcgcgtcag cctggacgat attctgcagc gcgctgatgt ggtgacggtg gggattgacg
     2281 gcgggggcct ggatgatctg ctgggaatgt acgtgattgg ccgtgacagg gaaacccgcg
     2341 aatggctggg ctggggccat gcctgggcgc atgaaacagc ggtggtcaga cggaagagtg
     2401 aggcatcccg ttttcaggat tttgtggcct gtggagacat gacgattgtc cgtcgggtcg
     2461 gggatgacac ggcggaagtg gcggagtatg tgcgtcgtat tcatgaggct gagttactgg
     2521 atcatatcgg tattgacccg tcaggtgtgg ggcagattct ggattcactg gcggaagccg
     2581 ggatccccga cggaattgtt gtggggataa gccagggctg gaagctgggc ggggccatca
     2641 aaaccaccga gcgcaaactg gctgagggag tgctggtgca tggtggtcag ccactgatgg
     2701 cctggtgcgt tggcaatgcc cgggtggagc ctaaaggtaa cgccatcctt attaccaaac
     2761 aggccagcgg acgggggaaa attgacccgc tgatggcgct gttcaatgcg gtatccctga
     2821 tatccctgaa tccggagccc aaaaagaaag aatatgcggt ttttttcata taaccctgtt
     2881 caccctgtaa ccatcatgga ccgctgcggc ggttttttta ttttcaggag gctgatgtga
     2941 ctcttaaacg ggcctgctcc ctgctgacgg tgaaatcctt cagtgaggat gagcgggtga
     3001 tcaccgggat tgcgtcaacg ccttctccgg atcgggatgg tgacatcctg gagccggagg
     3061 gcgcggagtt tggcagtgcg atcccgtttc tctggcagca tgaccattcc cgcccggtgg
     3121 ggcagtgtac ggtgcgccgg gtcagcgaag ggc
//
LOCUS       DAZFFI010000239         3122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFFI010000239 DAZFFI010000000
VERSION     DAZFFI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.241"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..720
                     /locus_tag="ACHJ12_005013"
     CDS             <1..720
                     /locus_tag="ACHJ12_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4992649.1"
                     /translation="HVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGA
                     GLYASALFDSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTE
                     DAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFE
                     KSAFGKYNVDNAVNASFRYSF"
     gene            complement(1143..1301)
                     /locus_tag="ACHJ12_005014"
                     /pseudo
     CDS             complement(1143..1301)
                     /locus_tag="ACHJ12_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            1450..1575
                     /locus_tag="ACHJ12_005015"
                     /pseudo
     CDS             1450..1575
                     /locus_tag="ACHJ12_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1635..2141)
                     /gene="yciE"
                     /locus_tag="ACHJ12_005016"
     CDS             complement(1635..2141)
                     /gene="yciE"
                     /locus_tag="ACHJ12_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4992650.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2187..2687)
                     /gene="yciF"
                     /locus_tag="ACHJ12_005017"
     CDS             complement(2187..2687)
                     /gene="yciF"
                     /locus_tag="ACHJ12_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4992651.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2773..2952)
                     /gene="yciG"
                     /locus_tag="ACHJ12_005018"
     CDS             complement(2773..2952)
                     /gene="yciG"
                     /locus_tag="ACHJ12_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4992652.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT          830 a          658 c          706 g          928 t
ORIGIN      
        1 cacgttcagg tgggtgcgga taaaaaacac gaactggacg gtctggacct cttcaccggg
       61 gtgaccatga catataccga cagcaacgcc ggcagcgaca ccttcagtgg taaaacgaag
      121 tcagtgggtg cgggtctgta tgcttcagcc ctgtttgatt ccggggccta tatcgacctg
      181 attggtaagt atgttcacca tgataatgag tatacggcgt cctttgccgg actcggcaca
      241 aaagactaca gctctcattc ctggtatgcc ggtgctgaag tgggttaccg ctatcatgtc
      301 acagaggatg cctggattga gccacaggcg gaactggtct atggtgctgt atccggtaaa
      361 cagttcagct ggaaggacca gggaatgagc ctgtccatga aggacaagga ctacaatccg
      421 ctgattggcc gtactggtgt ggatgtgggt aaatcgttct ccggtaagga ctggaaagtg
      481 acggcacgtg ctggtcttgg ctatcagttt gacctgctgg cgaacggaga aacggtactg
      541 cgtgatgcat ccggagagaa gcgtattaag ggcgggaaag acggtcgcat gctgatgtct
      601 gtgggtctga acgcagaggt tcgtgacaac atccgttttg gtctggagtt tgagaaatca
      661 gcctttggta agtacaacgt tgataatgct gtcaacgcca gcttccgtta ttcattctga
      721 cggttgttac tgaaggggat tatggcctga cggctccctg taaataaaga caggcacaga
      781 agattgtgag ttacttagtg ttccaccaaa aagtaaggtc gcaactctgt gcctgcttcg
      841 taagtgtaca gtgagggcca tatggtcata actgaagcat ccgacgatgt ggtgtctacc
      901 caaaaagcga cctgtgaata ttaggctggt taagtgcgta ttttcatgga aggagatcac
      961 ccaatagccc cgatctcctt tatgttaata cgcattaagc gggtaaaaca ggaagattaa
     1021 gaaagggaga gtttttcgac tccccaacca agagaagcag cttataacta ccagatagtt
     1081 gttatgtcat acgtgacgga taatgtcata aaaaatatgt cattgccgtg attataatcg
     1141 cgttattgga taaagcagtc ttcagcggtt tcccaggctt cctgcagcgt ttcctgtaca
     1201 aattcgtgcg cggattcttt atttacaact cttaaaatgg tgagtccatc attgctggtg
     1261 gttctgacaa tcacgtccac atggtcatat ccgttattta tggaatgact ataagggggg
     1321 ataagcctct gcaaaacaaa aaaagacttt aacaaaatca atcagttaaa aacacagaaa
     1381 aacggtatga aaaatcacca taaaagtttg tttttccttt tataaatagc tgcttatcac
     1441 attttttata atctaccagt gtggtacatg gatatcgata ccaccgccaa ttataagcta
     1501 ggcggtgcac agcaacacga tagcgtacgc ctcgatccgt gggtgtttat gttctcagca
     1561 ggatatcgtt tttaattccg cacaaaaacg accccgtaat atacggggtc aataaggaca
     1621 tggtataaag ggtattattt cttcgcttct acgccatcag tttcagaacg aattaaaaat
     1681 ttctcagttg tttgcggaat atgctgaatc agccagtcgg ccatttgctt ttcctcattt
     1741 aaaatcgctt cgatgatcgg aattgagact gtatcaccgg cattttttgc tgctgctaat
     1801 agtgaggtgt aacaggcgat ttcaaattgc tcgaagacat atccgctaat agagccttta
     1861 actatttcat cagaagggaa tataccaccg attgactgcc caagcgcagc cattttactc
     1921 atggaatctt taatgactga acgtgaaatg tcattacgat caagaatagt ttccagttga
     1981 acaatctggt ttttggtttc actaagatgt tgttcaatac gagcgcgtag ttcaggataa
     2041 ttatctatac gactggccat ggattcaagc atagattcgg cttgcttttc cattgcgtgg
     2101 gcgtcacgta accagtcatg ataatgttca atacgattca tgaaaatgtc ctctttataa
     2161 aatgactaaa agttaaaatt catatttcag gctttatttt cggctttctt atttacgtta
     2221 ttaagggcca gatcagtcag tttgatgtcg gtggcctttt cttcttccag ggtttctttc
     2281 aaaagcttcg ctgctttacg gtaacctaat tgttcagcca gcgtcgctaa tgtcccgtaa
     2341 ctggcaatct cataatgctc gactttctgt gctgcggcaa tcagtgcggc atcacgcact
     2401 tcgtttttct cggtactttc gatgacctca ttagcttctt caataagacc ttccattgcc
     2461 acacatttca tgcgcttaat tttcaggttc gattcggact ccacaacttg atcaatacgt
     2521 tcaatctgtc catgagtttc ctcgaggtgc gcatgaaaag cctgacttaa tttttcattt
     2581 gatgttgctc ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc gctgtaggta
     2641 tctgaaagca ggtgaataaa tacatcttca atggtcttca tattcatatt tttctccagt
     2701 gaaatcactg cgagccatac ggcccgcaga tgcaattatc tttatcagct gatattaata
     2761 attaacggtg aatcaggatt tacctgattt attaccaccg ctttgttgac cgcctttttt
     2821 acccgcttca gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat gctgaccgcc
     2881 tttacggcct gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg aaccaccacg
     2941 atgttcggcc atgttatttc tcccgttgcg ttgcattgtt tcattaatat gagtgttgtg
     3001 tgtcgacact cattaaaatt agtcgctaat gagaattagt caaattaagc gcaacgagaa
     3061 gatagaggga aaatatattt tgaggaacat tctggatata ttaacaatta cctgaggaat
     3121 aa
//
LOCUS       DAZFFI010000240         3063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFFI010000240 DAZFFI010000000
VERSION     DAZFFI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.242"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            712..1818
                     /locus_tag="ACHJ12_005019"
                     /pseudo
     CDS             712..1818
                     /locus_tag="ACHJ12_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1921..2229)
                     /locus_tag="ACHJ12_005020"
                     /pseudo
     CDS             complement(1921..2229)
                     /locus_tag="ACHJ12_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2246..2926)
                     /locus_tag="ACHJ12_005021"
                     /pseudo
     CDS             complement(2246..2926)
                     /locus_tag="ACHJ12_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          822 a          714 c          734 g          793 t
ORIGIN      
        1 ttacaatgaa cagaaaggaa ggtaaataac atttagctca ttcaggtaac actttgaacc
       61 tgtccgactt gatgagaatc agcccctttc tacgagggac ttcttactgt ataatttata
      121 gtcctgtcga agaggtaaaa tctttcaacc ggatatctcc ataaacagca ttccagtaaa
      181 tcaagcaaga gtgaacagga gactggtgct gtttattacg gtatgcttgt ccgatgtggt
      241 ggaaaataaa aatgggcgac tgaccagata tgggagtctc cggtcagtca ttcgcttgta
      301 gatgcaggac ataagcgaac caaggcccac tgcttctgcg caaaagcaag ggggagttta
      361 ccctggcaga ttgagtttcg cgtcactgac cagtcagagc tccagactca tcttacacca
      421 ctaaccccct taccactcct gaatcttccc tcggcaacag ccaagacagt atcgtactgt
      481 tcaccaccat cttttcccgt gaatgacagt aagtgcaaag ccccaatata aatcagtact
      541 tccgatgaca gacagtgaag gcgtaaggtg aaatatgcac gtaaggcagt atgactttcc
      601 gtgagacaga tgctgtccaa cgtgggatat ttaatgcggg ttaacatcgt tgtcagagcc
      661 gcatgggtga gctgttgcta cccccctgga atttccacca cggaggccag catgaacaac
      721 aataacatgt tgtacgtcga attagatgtt cacaaagaat cgattactgt ctcctatgct
      781 atcaattcag aacctgttga actgatgggt aaaattagca catcacctac tgacattcat
      841 aatctttatc aacgtatcag gtcaaagtca tcgcaggtca gtatcgttta taaagcggga
      901 ccctgtggtt atggacttta tcgtcggctg gtgaaatccg gttttgactg catggtctgc
      961 gccccctcac ttatcccgaa aaaaccgggg gagcggggta aaaccaacac cgtgatgcca
     1021 tcagacttgt gcgttcactg cgtgcaggag atctttctgc tgtctacgta tccggcgttg
     1081 aagatgagac attccgggat ttggcccggg catgggcatc tgcccgcgat gatttacggc
     1141 atgcaaggca acgtctgaaa tcgtttcttc tggttcatgg ggtccattat gtcgggcgag
     1201 cagattgggg ccctgcacac cgacactggc tcagcaaata ctcattcgaa agtccctggc
     1261 gacaattagc ttttgacgaa caccgttgta caatagaaga ccggcaggca caatgtgaac
     1321 ggctggaatc cgcccgaagg aggccgttac cgaatggagg ctgtatccgg tagtagaggc
     1381 gcagcaagcc atgcgaggga ttcagtttat tactgcggtt ggattaattt ctgaactggg
     1441 tgatctgacc cgttttgaac atccacgaca actgatgtca tggtttggta tcacgccatc
     1501 agaatattca tcgggtggaa gcagacatca gggcagcatt accaaagccg gaaacagcta
     1561 tgccagaaag ctattggttg aggcagcgtg gagttaccgg catccgacac gcatcagtcc
     1621 ggcaatacaa aaaatcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
     1681 caactcaggc tttgcaagag gtctcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
     1741 acaattgtcg ctgtcgcacg tgagcaggtg gttttatctg ggatatgggg cgggatagcc
     1801 atgtttgtcg cacaataacc acaatgtcac aaataaagaa tgaggataac cagttccctg
     1861 aaggtgacgt agcccagaac aagagtaact catgttagcg tctggcgatg gctgaagtgg
     1921 tcaacaaaaa ctggccaccg agttagagtt tttccagtat cgattttccg attcgtttgg
     1981 ggataaccca ctgttatatt cgtgcggtct tagtgcgctg taatatccaa cgatatcgtc
     2041 cgttatggcg tgagctgcct cgctgaagct tacgtaaccc accaccggca tccattcgtt
     2101 cttcagactc ctgaagaagc gttccattga gctgttatcc cagcattttc cgcgccggct
     2161 catactctgc ctgatctggt atcgacacaa taactgccgg aactgcctgc tcgaataatg
     2221 actgccttgg acgcccttct aagagtcaag acgttatcgg gatgcagtta acatgcctgt
     2281 tttgatttcg tagtaatgta taaacttcgc gtgagatata gcgcttcaga cagcgtattg
     2341 cttccatttt tgtatgtcct tcggctactc gcctggagac atattccttt gttttatcgt
     2401 cagtttgtaa acatcctata gtgatgatgt aaagtgcact atttgcaaca cgctccccac
     2461 cccgattaag tcggtaatgg ttcgtttttc cagaagatag gggaaactgg ctgacaccgc
     2521 acaacgccgc aaaacctgat tcggttctta accgttaggg attgtctccg gcagagatca
     2581 acaactgcaa agcgctttcg tatccaacag cattacgttt aatcagttca ggtgccagtt
     2641 catcaacaat tgccgcaatc atgacatcca agttggtgat ttcatcatga aactcgaaat
     2701 agcgtcgaac aagggatttc aatgtaatgc gataaacgtt ggtaacatta ctgtattcac
     2761 tggcatcagg cctccaggat cccagagtcc tgataagttg catatgtgtc atatttctga
     2821 gttgttcgag taattcatcc ggtgcagaga taatatagga atggataatc tggagagcgc
     2881 ctctgcgggc tgatattgcg gttttgcggc aaatttttag cacccgctac tacgccaaat
     2941 acctgaagta gaaatggatg tgtcacgaac gatacaatat tgggccgttg gtggagatat
     3001 aagtggataa cttttcatcc gtcgttgaca ggatgcgtta gcagcacaca tccggctctg
     3061 cgc
//
LOCUS       DAZFFI010000241         3007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFFI010000241 DAZFFI010000000
VERSION     DAZFFI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.243"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(186..262)
                     /locus_tag="ACHJ12_005022"
     tRNA            complement(186..262)
                     /locus_tag="ACHJ12_005022"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Asp,seq:gtc)
     gene            complement(395..1126)
                     /gene="dnaQ"
                     /locus_tag="ACHJ12_005023"
     CDS             complement(395..1126)
                     /gene="dnaQ"
                     /locus_tag="ACHJ12_005023"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4992653.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1191..1658
                     /gene="rnhA"
                     /locus_tag="ACHJ12_005024"
     CDS             1191..1658
                     /gene="rnhA"
                     /locus_tag="ACHJ12_005024"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4992654.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1655..2377)
                     /locus_tag="ACHJ12_005025"
     CDS             complement(1655..2377)
                     /locus_tag="ACHJ12_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4992655.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2411..>3007
                     /gene="gloB"
                     /locus_tag="ACHJ12_005026"
     CDS             2411..>3007
                     /gene="gloB"
                     /locus_tag="ACHJ12_005026"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4992656.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLKAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPPETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKN"
BASE COUNT          814 a          721 c          711 g          761 t
ORIGIN      
        1 ggaaatatat ttaagctgaa tggttcgata tcattattga attttaatga atgagacaac
       61 aagcgagaat cacgcactta cgccagaata aaatcattga acagagaaat ggtggaattc
      121 aaatacaaaa aaaccgcaaa attaaaaatc ttgcggctct ctgaactcat tttcatgagt
      181 gaatttggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc
      241 taaccgactg aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga
      301 ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc
      361 ccaagccgct atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc
      421 ccttcttctc caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa
      481 aaacaacgcg taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt
      541 gctgctgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca
      601 gataaacttc cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt
      661 tatctatttc gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt
      721 tcctcgccac cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc
      781 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
      841 ccaactccgc gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct
      901 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
      961 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
     1021 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg
     1081 tggtttcggt atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
     1141 tcagacttgt cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac
     1201 aggtagaaat tttcaccgat ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg
     1261 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca
     1321 acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg
     1381 aagtcatttt gagtaccgac agccagtatg tccgccaggg gatcactcag tggatccata
     1441 actggaaaaa acgtggctgg aaaacagcag acaaaaaacc agtaaaaaat gtcgatctct
     1501 ggcaacgtct tgatgctgca ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc
     1561 atgccggaca cccggaaaac gaacgttgcg atgaactggc tcgtgccgca gcgatgaatc
     1621 ccacactgga agatacgggc tatcaagtcg aagtttaagc ctgtggttta cgacattgcc
     1681 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta
     1741 gcgttaaagg aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa
     1801 tatgcgcatt caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg
     1861 cgtgcagcac ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca
     1921 tccggctgtt atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta
     1981 aactgatggg attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg
     2041 cttcacgcaa taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa
     2101 catcaacgga tttatcggca aaaggaagat gaagtgggtc cgcctgtacc tggacgggca
     2161 ttccttgcgc agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg
     2221 cgcttaaatt gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga
     2281 gctggcgctc cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcaatcag
     2341 gagccacgac agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga
     2401 gaggtaatct atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt
     2461 gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cggtattaaa
     2521 agccattgcc gccaataact ggcaaccaga ggccatattt ctcacccacc atcaccacga
     2581 tcacgttggc ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc
     2641 accagagaca caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt
     2701 tttggggcat gaatttagtg taattgccac gccgggtcac actttaggac atatctgtta
     2761 cttcagtaaa ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt
     2821 gtttgaaggg acagcatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga
     2881 cgatacattg gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag
     2941 tattcttccg cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc
     3001 aaaaaat
//
LOCUS       DAZFFI010000242         3000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFFI010000242 DAZFFI010000000
VERSION     DAZFFI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.244"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            929..1528
                     /locus_tag="ACHJ12_005027"
     CDS             929..1528
                     /locus_tag="ACHJ12_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4992657.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGIYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEAGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSTLPERCSLV"
     gene            1899..2282
                     /locus_tag="ACHJ12_005028"
     CDS             1899..2282
                     /locus_tag="ACHJ12_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4992658.1"
                     /translation="MYAKSFIALDGNGHLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERREVRLIKRLQKFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCHTGHFSEEGVAE"
     gene            2279..2704
                     /locus_tag="ACHJ12_005029"
     CDS             2279..2704
                     /locus_tag="ACHJ12_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO4992659.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRATCTKSVYSYQPDLFQDCGGAST"
BASE COUNT          735 a          730 c          746 g          789 t
ORIGIN      
        1 atgaactctg taattctatc ctgtaattct atcctgtaat tctatcctgt aactctcccc
       61 tgtaattgta cctcgtaaat acaccttgta accaggtcct gcagaagtct gtctcataga
      121 agtatctgtg cagcgatatt ctctgtagta cttgtaacac tgtccttaac cctctctgct
      181 gcaatgtaat cccgtaacac ctgaatataa atctgtaatg tacacacact gactgtaacg
      241 tactttgtta agtaatgcct gttgtgtgga gactattctg ggtccgccat tgcttttgtg
      301 cgtttgtgta agtgtttctg gctaaataag agtatgactt tgccagtgtt gatactgtgg
      361 ataacggtga ttaaggaatt actgttaatg atctgttcct gcagcttacc tgatgagttt
      421 tggtagtaat acgtaaaaga gtatgcctta taccattaaa aacagcctta cggcgtgttg
      481 tttaaccctg tacctctgtt acttatgtag tacgttgcgg aaaactacgt taattgtcat
      541 atcgggttat ggctggtatt aaaagcattt tttaccggag aaacagggaa gtggaaaggc
      601 tttagggata acgtcaggcc attctgacgg tattacgtta atcacagtac aggataacgg
      661 gagggtaatg gaattttaaa ccagagtagt tccgctgttg ttcactggaa aaagtgattg
      721 cccacacgcg ttataaacgc cttcccgctt cagtctggtt accagagctt caggcgttat
      781 atgcgctgta acgggctgtt acgtatgctg ttctctgtct gcttctgata cccgtggacg
      841 tcgttaccgg aggtcaccct gcctgataac gttttcttca aatttacggc aacgcgtctt
      901 cctgtgttac agaacacgag gaagaaacat gaagacttac aacaccaatc ccggttacga
      961 aatggatccc cagcttctga cacactttaa ccagcatctg gattcattat tcgggatata
     1021 ttcgaaactg ttaccgttca gaatggattt tgcttaccga aaaaacaccc tcagctacag
     1081 gtgtgcctgc aggtacgcca tgtgcgcgga gatactgcga ctgattaacg aagcgggaga
     1141 gaaactggtc gggtatgcct gggtaatgga atatacggaa cgtaaagggc ttcacattca
     1201 ttttgtgggc tatctgaacg gtcagagcca caggagttcg tatctggtat ccagattgat
     1261 gggagatatc tggaggcggg ttaccgaggg tgatggttac taccactggt gccggtttaa
     1321 taaaaattat ccggtaaaca ttaaccacgt cattcactat tcagatcaca aagcggtgaa
     1381 tgccttacgc tatgccctca gctacctggc caaacgagaa cagaaagaat gtggcatcat
     1441 cctgaaatgc agcgggcttc ctgaaaaaag taatcggggg cgaccgcgac tgggcagcac
     1501 cctgccggag agatgttctc tggtgtaagc ccctgaacga cagctgcggg ctggcgctac
     1561 tcgttacagt cggttccgga ggccacttca gcggcactgc acatttttta cgacatggct
     1621 attccgtatg aaccatacgg agatttaacc atggcacatt ctttaccagt ccttccggca
     1681 accgatacag cacattctct gatgtccatc cctgcctgct gacagttttt tacgccggtg
     1741 ggccattgtg tgacaaccac agtactgtaa cagcaaccag cctgctttac ccaaactcca
     1801 gtccagattt taactgaacc gccattcaca cctgaccaca ctgcccgtac cgggacggct
     1861 gttgcctgcg tgtcgtctgg cggctgagga atatgacgat gtacgcaaaa tcctttatcg
     1921 ctcttgatgg caacggacat ctgacgggcg cccgtactgc acaggccgca ccttatgctc
     1981 actacacctg ccacctttgc ggcagtgcac tcagatacca cccgcagtac gacactgaac
     2041 ttccctggtt tgaacacact gacgacgggc tgactgagca cggtcaacag tgcccttatg
     2101 tcagaccgga gcgcagggag gtacgcttga ttaagcgtct gcagaaattc gtaccggatg
     2161 ccttacccgt ggtgcgtaaa gccagctggc actgcagaca atgtcatcac gattattatg
     2221 gggagcagta ctgcactcac tgccatacag ggcacttcag tgaggaggga gtggctgaat
     2281 gaacggtata aaaacggggc tggggatcac gccgggtgaa cacattatca gtgccgactc
     2341 tgcgctcagc agaaatatcc ggcagtgttt ctgcctttcc tgtcgtggcc ggttgatttt
     2401 gcagacagat gctcaggggg cgtggtttga gcatgattta catgccctga gcgcgcaaca
     2461 gaaggcggcc tgcgtggtgc tgaatccgga aaagtcccac ccttacattg aggatatggc
     2521 gatgtttcta tcgccattac cggtcgtgct ggagtggcat tgtgtgatgt gcgggcagca
     2581 ttttcccggg aaaaagttct gcgaagcctg tggcacgggg atctattcca gagcaacatg
     2641 tacgaagtct gtttacagct accagccaga cttgtttcag gactgcggag gtgcgagtac
     2701 ttaatccaaa ctatccgctc tgcgcagggc gttcagaggt attgccagcc tgcattttat
     2761 ccggtgtttc gcacctaccc cgttcgtgac acaaaggcgc gacaccggat atccgcaatc
     2821 ctgcttacct gaataaaacc cggggtcatg attgtgtgct gtggaacgaa atcgcaccgt
     2881 gatcgcggat tcgccgaatc tgcactgtgg tgtcaataaa ccacacgcga gagtcctctc
     2941 cgtattgatc agtgcatcag gacgatttct gcattgttaa tccagggggc tgcattattc
//
LOCUS       DAZFFI010000243         2972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFFI010000243 DAZFFI010000000
VERSION     DAZFFI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.245"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..1294
                     /gene="bluF"
                     /locus_tag="ACHJ12_005030"
     CDS             83..1294
                     /gene="bluF"
                     /locus_tag="ACHJ12_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO4992660.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1499..2230
                     /gene="bluR"
                     /locus_tag="ACHJ12_005031"
     CDS             1499..2230
                     /gene="bluR"
                     /locus_tag="ACHJ12_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO4992661.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2451..2855
                     /gene="ycgX"
                     /locus_tag="ACHJ12_005032"
     CDS             2451..2855
                     /gene="ycgX"
                     /locus_tag="ACHJ12_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO4992662.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2908..>2972
                     /locus_tag="ACHJ12_005033"
     CDS             2908..>2972
                     /locus_tag="ACHJ12_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992663.1"
                     /translation="MGIKVQRLSLWMINTVLLLLP"
BASE COUNT          874 a          629 c          640 g          829 t
ORIGIN      
        1 tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct
       61 cacccattac cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga
      121 cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca
      181 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
      241 tccggaagaa caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa
      301 tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga
      361 attatttgat ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
      421 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
      481 ccttgcaacc gaacaatcaa cctatttcga gatccctgcc gaagactcct ggctttttat
      541 cgctgacgga tctgataaag aacttgattc ctgtaccctt tcaccaacta taaacgacca
      601 ctttgccttt catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat
      661 tgtgcaaaaa aatgaagata gcccatcagc catagcggtt gggcagcgta aagacgggga
      721 aatctacaca gcggatctca aaagtaaggc gcttgcattc gcgatggcac acgcacttga
      781 gctcggtgat aaaatgattt caatcaatct attacctatg acccttgtta acgaacctga
      841 cgcagtctct tttttacttg atgaaataaa ggccaatgct ctggtgcctg aacaaatcat
      901 cgttgaattt actgaaagtg aagttatatc tcggtttgat gagtttgcag aagcgattaa
      961 atcgcttaag gctgccggta tcagtgtagc aattgatcat tttggcgcag gttttgccgg
     1021 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     1081 caatgttcat aaaagcgggc cacggcaggc aattattcaa gcgatcataa aatgctgtac
     1141 atcacttgaa attcaagtca gtgctatggg cgtggcaaca ccagaagagt ggatgtggct
     1201 tgaatccgca ggaattgaga tgtttcaggg agatctgttt gctaaagcta aattgaatgg
     1261 tatcccttcc atagcgtggc cagagaaaaa ataattttca gcacattctt tcacatgatt
     1321 tcagtaaatc atcgtgaaaa tgaatacgtt acgtttaaat ttaatccaca ttaaaaataa
     1381 tgtttcatcc aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt
     1441 acaaagtatt attgggtcgt gtacaggcga cggagatttg tgaccgcaag gaggaattgt
     1501 ggcttattac agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg
     1561 ggcctggcaa cgccgctacg gtttgttaaa accacagcgc agtgaaggcg gacaccgact
     1621 ctttgatgaa gaagacatac aacgcatcga agagatcaag cgttggataa gtaatggcgt
     1681 ccctgtaggc aaagttaaag cattactgga aaccaccagc caggatacgg aagatgactg
     1741 gagccgcctg caagaagaga tgatgtcaat tcttcgcatg gctaatcctg ccaaactacg
     1801 cgcgagaatt atttcactgg gtcgagagta cccagtagat caattgatta atcatgttta
     1861 ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta tgagcagtat
     1921 gtttgacggc gcattaattg aatacgcagc aacctcgctt ttcgaaatgc gccgtaagcc
     1981 cggtaaagaa gccattctga tggcgtggaa tgttgaagag agagcacgat tgtggctgga
     2041 agcatggcgt ttatcattgt caggatggca catttctgtt cttgctgatc ccattgaatc
     2101 gccgcgcccg gaactgttcc cgacgcaaac attgattgtc tggacaggca tggcaccaac
     2161 gagaaggcaa aacgaacttt tacaacattg gggtgagcaa ggttataaag tcatctttca
     2221 tgccccctaa tttcaccttt atcccgcccc ttagacagaa tgttataatc tgaacccaca
     2281 acagcgtttg taccggagct ggtccgttgc aaaatgctca ccgggagaat tcagatattc
     2341 cctctatcac actctgtaca tatcacagcc aaaggacaga ccagattgaa gtggtaaaac
     2401 cgatcgtgca cactacgcag tctcacaact aacaagaggt tattatcctg atggatcagg
     2461 tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga gcaaaacttc tcctggtttt
     2521 attcagaatt aaaacatcac cgtattgcac attacattta ttatctggct acggataaca
     2581 tcagaattat tactcacgat gacacggttt tgttattaag aggaaccagg aacctgttaa
     2641 aagttagtac gaccaagaac cctgctaaaa taaaagaggc cgcattgctt catatttgcg
     2701 gaaaatctac atttcgggaa tactgttcaa cactagcagg cgcgggcgtt ttccggtggg
     2761 ttactgatgt taatcataac aaacgcagtt actacgccat tgataatacg cttttatata
     2821 ttgaagatgt agaaaataat aaaccattaa tctagctaaa gttggatgct taagaaatgc
     2881 ttcataattc agtaaggcat tagcataatg ggaataaaag tacagagact atccctatgg
     2941 atgataaata ctgtcctttt attgctaccc at
//
LOCUS       DAZFFI010000244         2782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFFI010000244 DAZFFI010000000
VERSION     DAZFFI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.246"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..332
                     /locus_tag="ACHJ12_005034"
     CDS             144..332
                     /locus_tag="ACHJ12_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4992664.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            476..1324
                     /gene="mscS"
                     /locus_tag="ACHJ12_005035"
     CDS             476..1324
                     /gene="mscS"
                     /locus_tag="ACHJ12_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4992665.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIIGGIAARMISGVLNNLMLGRN
                     IDATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNL
                     AAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIV
                     NFSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMN
                     FVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSR
                     SVSSRQ"
     gene            1450..2670
                     /locus_tag="ACHJ12_005036"
     CDS             1450..2670
                     /locus_tag="ACHJ12_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO4992666.1"
                     /translation="MPKKIMTEIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
BASE COUNT          696 a          515 c          763 g          808 t
ORIGIN      
        1 ttacgggtac ataatgaagt tgagttgttg tacaagggga tgcttctggt cagaaaaggg
       61 aggttgtgaa gcaggacagc ttatttccgg taatgttaaa cgcagcctct gattcgttaa
      121 tttcataatc ataaggagtt tttatgcgtc tgttgattgc ttttcttctt ccctggctga
      181 cttttttcac catcggccgg cctctgacgg ggattttttg tctggtgtta cagataacgc
      241 tgctgggctg gataccggca acaatatggg ccgtgtatgc gctttcgcag tataagacgg
      301 aacagaaaat ccgcgaagcc acggggaaat gatgactgca gaagagcggg ttgtcatagc
      361 tgtgtatatg atgcgggata aaggcccgga gattcctgga aataaaatgt tccggggaat
      421 ataaaacagg gaaaaaccgg atgtgatgga cgcttctggc gaggcggttt catgaattgt
      481 ccgtaatcag gatatgttgc ccggttacgc cgcgaatctt gtggcagcag tggttattat
      541 tattatcggg ggtatcgctg cacggatgat ttctggtgtg ctgaataatc tgatgctggg
      601 caggaatatt gatgccacag tggcgctgtt cctttccgcg ctgatacgtc atgccattat
      661 agcctttacg ctgattgctg ccctgggacg tgtgggggtt cagacagctt ccgtaattgc
      721 agtcctgggg gctgcaggtc ttgctgttgg acttgccctg caggggtcat tgtcaaacct
      781 tgcagctggt attcttctcg tgatgttccg tcctttcagg gctggtgaat atgctgacct
      841 gggtggtgtt gccgggactg tgcagaatgt acatatattc tcaaccacga tgcgtacact
      901 ggatggtaaa attgttgtca ttccgaacgg gaagattatt gccggagaga ttgttaattt
      961 ctccagggag cctgaacgac gaaatgagtt tatcatcagt gtttcatatg atgcagatat
     1021 tgactgtgtg aaacaagtcc tgacagatat tgtgatgtca gaagagaggg ttctgaagaa
     1081 cagggaaatc actgttcgcc tgaatgagcc tggggagtca tccatgaatt ttgtagtacg
     1141 ggtatggagc aggcgtgatg accttcagtc tgtatactgg gatgtactgg agagaattaa
     1201 gcgggagttt gattcagaag ggataagttt tccatatcca caaatggata tccatcttgt
     1261 tcgctccggg aagcaggcag agtcggtaac ccataacagt cgctcagtca gttccagaca
     1321 gtaaaatgaa aagcgcattt tatttgtgaa agatgaacag cctgaatctg tcaggctgtt
     1381 ttcattgtgt ttttatggtc atttttggta tatgtactgt atataatgtc atctgtacaa
     1441 ggatataaaa tgccaaagaa aataatgaca gagattgatg tttttgacgc tgccatggag
     1501 cgtattgaat gggtgtttga tacgttttca tcagtttgtc tttctttttc cggcggaaaa
     1561 gactccactg tcctgtttca cctgatggcg aatattgccc gccgaaaaaa acgccgtttt
     1621 tctgtactgt ttgttgactg ggaggctcag tatcagtgta ccattgagca tgtcaccagg
     1681 ttgagggaaa tgtacgatga tatcacagat acttttttct gggtggcgct gcctctgacg
     1741 accgttaatg gagtgtctca ggtacagcct gagtgggtat gttgggagga cggggctgaa
     1801 tgggttcgcc agccaccaga agatgccata accagagcgt ctttttttcc tttttatcag
     1861 aatgcgatga catttgaaga gtttattccg gcatttaatc attggtttgc ccgggaaaaa
     1921 gggatggttg ctgcgattct tactggtgtt cgcgctgatg agtcactgaa tcgttttatg
     1981 ggactggtat ccggacgtaa gctccgttat gcggaggata aaccctggac cacggcatcg
     2041 gctgagggat tttactacac catgtatcct ttgtacgact ggaaaacaag agatgtatgg
     2101 atttaccatg cgagaacacg tgctgcctat aactctcttt atgacctgat gtacagggct
     2161 ggagttcctc ttcgcaatat gcgggtgtgt gagccttttg gaccggagca acgtaagggg
     2221 ttgtggcttt atcatgtact ggagccggat acatggggca gaatgtgtgc tcgcgtatca
     2281 ggtgctgtag ggggagcgct ctatgcaaat gaaagtggtg cttattttgc tttgaggaaa
     2341 cagataacaa aaccagttca ccatacatgg cagagttatg caatgtttct gctggagtca
     2401 atgcctgaga aaacggcgga gcattacaag aataagattg ctgtttatct tcggtggtgg
     2461 cagtctcatg gatggccgga tggcatccct gatgagcagg ataatgacca gggatgtaaa
     2521 gatattccct cctggcgtcg catctgtaag acgttactga aaaatgattt ctggtgtcgg
     2581 atgctggcgt ttagtccgaa taagccccga cattatgagc gctacctggc cagaatgcgt
     2641 gaaaaaagga aagagtgggg aatattgtaa attatattta aacatcagaa atatgacggt
     2701 taatccagga tagtagctaa aaaaataaga ttccttgctg atttatccct gtttgatatg
     2761 taaaatctga ggggcttata ag
//
LOCUS       DAZFFI010000245         2749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFFI010000245 DAZFFI010000000
VERSION     DAZFFI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.247"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2640
                     /locus_tag="ACHJ12_005037"
     CDS             <1..2640
                     /locus_tag="ACHJ12_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992667.1"
                     /translation="TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAV
                     HREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWG
                     KAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHY
                     DLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHR
                     VAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ
                     HLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDE
                     RTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSR
                     KPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQ
                     QHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQ
                     LLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHA
                     GIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVG
                     IGAGVDASVMTLACRTWQECWVN"
BASE COUNT          666 a          707 c          869 g          507 t
ORIGIN      
        1 acgacaacgg atgcgacggg cagcacccgg cagatgacgt ggagccgcta cgggcagttg
       61 ctggcgttca ctgactgctc gggctaccag acccgctatg aatacgaccg cttcggccag
      121 atgacggcgg ttcaccgcga ggaaggtatc agcctttacc gccactatga caaccgtggc
      181 cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc agtatgagta caacgccgca
      241 ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca gtacgatgcg
      301 tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga gtacgatgct
      361 gccggacgtg tcatcagtct gactaacgag aacggcagcc acagcgactt cagctacgat
      421 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
      481 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      541 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      601 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      661 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      721 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      781 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      841 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      901 atgcaccgtg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      961 ctgaccagca catacacccc cgcaggccag ttacagagcc agcacctgaa cagcctggtg
     1021 tatgaccgtg actacgggtg gaatgacaac ggcgacctgg tgcgcatcag cggcccgcga
     1081 cagacgcggg aatacggcta cagcgccacg ggcaggctgg agagcgtgcg taccctcgca
     1141 ccggacctgg acatccgcat cccgtatgcc accgacccgg cgggaaaccg cctgccggac
     1201 ccggagctgc acccggacag taccctcacg gcgtggccgg ataaccgcat cgcagaggat
     1261 gcgcactatg tttaccacta tgatgaatac ggcagactga cggagaagac ggaccttatc
     1321 ccggcgggtg ttatccggac ggacgacgag cggacccacc actaccacta cgacagccag
     1381 caccgtctgg tgttctacac gcggatacag catggcgagc cactggtcga gagccgctac
     1441 ctctacgacc cgctgggacg gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg
     1501 acggggtgga tgtcgctgtc gcgtaaaccg gagatgacgt ggtacgggtg ggacggcgac
     1561 cggctgacga cggtacagac cgacaccacg cgtatccaga cggtatatca gccggggagc
     1621 ttcgcgccgc tcatccgcat cgaaacagac aacggcgagc gggagaaagc gcagcgccgc
     1681 agcctggcgg agaagctgca gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg
     1741 gctgaactgg tgcggctgct ggacaggctg gaggaagaaa tccgggcaga ccgcgtgagc
     1801 agtgaaagcc gggcgtggct tgcgcagtgc gggctgacgg tggagcaact ggccagacag
     1861 gtggagccgg aatacacacc ggcgcgaaaa gttcattttt accactgcga ccaccggggc
     1921 ctgccgctgg cgctcatcag cgaagacggc aatacggcgt ggcgcgggga gtatgatgaa
     1981 tggggcaacc agcttaatga agagaacccg tattacctgc accagccata ccgtctgccg
     2041 gggcagcagc atgatgagga atcagggctg tactataacc ggaaccggta ctatgacccg
     2101 ctacagggga ggtatattac acaagacccc attgggctgg cggggggatg gaacctgtat
     2161 aattacccac tgaatccgat aataaggatg gatcctttgg gtttgtataa tttatatcaa
     2221 ttattatatg atgtttggca tgatgattca tatggaacat catcaattga tattactggc
     2281 agtggagatc taatatcatt aggtggtcat gcaggacttg gcgttgcgtt tgctaaaaag
     2341 aaaggtgaaa tgttatctga tatttgtatt tatgctacag catgcggaca tgcaggaatt
     2401 ggtggtggga taaatgcggc tatcacatat tctgagacca agtctttacc tacatcggga
     2461 gtcagcaatt cagtaggtgt aacggttggc ggcggagttg gggggcattt tgcgtatact
     2521 tatgtagtgg atgttgataa tccagaatca tcgacagaat ctgttggtat cggtgcaggt
     2581 gttgacgctt cagttatgac tctggcttgt agaacgtggc aagaatgctg ggtcaattaa
     2641 caggaggaaa tatgaaaaaa tatatatttt tagttttatt tttattgtgt ggttgttctc
     2701 cagtggacat agagccagct agacaagaag ttaatatctt tcgtggttt
//
LOCUS       DAZFFI010000246         2741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFFI010000246 DAZFFI010000000
VERSION     DAZFFI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.248"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            171..1337
                     /gene="nhaA"
                     /locus_tag="ACHJ12_005038"
     CDS             171..1337
                     /gene="nhaA"
                     /locus_tag="ACHJ12_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4992668.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRAGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1403..2302
                     /gene="nhaR"
                     /locus_tag="ACHJ12_005039"
     CDS             1403..2302
                     /gene="nhaR"
                     /locus_tag="ACHJ12_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4992669.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2341..>2741)
                     /locus_tag="ACHJ12_005040"
     CDS             complement(2341..>2741)
                     /locus_tag="ACHJ12_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4992670.1"
                     /translation="IDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYYF
                     EPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQ
                     IPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          641 a          611 c          695 g          794 t
ORIGIN      
        1 tcacccgtta gcgcttacag ccgtcaaaaa ctcatctcac cgctgatggc gcaaatcctt
       61 taattgctcg taaaaaacga gttattccta cactataatc tgattttaac gatgatttgt
      121 gcggggtaaa atagtaaaaa cgatctattc acctgaaaga gaaataaaaa gtgaaacatc
      181 tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc
      241 tggcgatgat tatggccaat agcggcgcaa ccagtggatg gtatcacgac tttctggaga
      301 cgccggttca gctccgggct ggttcactcg aaatcaacaa aaacatgctg ttatggataa
      361 atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa cgtgaactga
      421 tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc gctattggtg
      481 ggatgattgt tccggcatta ctctatctgg cctttaacta tgccgatccg attacccgcg
      541 aagggtgggc gatcccggcg gctactgaca ttgcctttgc acttggtgtg ctggcgctgt
      601 tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg
      661 atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc
      721 ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc
      781 gcacgggcgt ctatattctg gttggcgtag tgttgtggac tgcggtgttg aaatcggggg
      841 ttcacgcaac cctggcgggg gtaattgtcg gcttctttat tcctttgaaa gagaagcatg
      901 ggcgttctcc agcgaagcga ctggagcatg tgttacaccc gtgggtggcg tatctgattt
      961 tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggtgtcacg ctggatggct
     1021 tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa ccgctgggga
     1081 ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga
     1141 cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact atgtctatct
     1201 ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg gcgaaactcg
     1261 gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta cgcgttcgtt
     1321 tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc ccttttcatt
     1381 gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt attacttctg
     1441 gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt taactccaca
     1501 aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat tatttaaacg
     1561 caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct atgccgataa
     1621 aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag aatccaattt
     1681 attgtttgac gttggtgtgg ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa
     1741 cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca cccacgaaat
     1801 gctgctggag caattaagtc agcataaact ggatatgatc atttctgact gtccgataga
     1861 ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg tgagtttctg
     1921 gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac ggcgactttt
     1981 gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta actcccaggg
     2041 attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc
     2101 gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt atgccgataa
     2161 aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc
     2221 tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt attctgcgct
     2281 ttttagtcca gcggcgcgtt aatcggcacc tccccccaaa gttaaggtgg gggagataga
     2341 ttagttgtac ataaccacga ttttgactcg gctcattatt tgcccgcttg agacattatt
     2401 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2461 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2521 aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2581 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acatcactgg gttcaaaaac
     2641 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gataattttc
     2701 attattgata cgagacagta caaatcgtcc agtgtcgatg g
//
LOCUS       DAZFFI010000247         2739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFFI010000247 DAZFFI010000000
VERSION     DAZFFI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.249"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..644
                     /locus_tag="ACHJ12_005041"
     CDS             177..644
                     /locus_tag="ACHJ12_005041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992671.1"
                     /translation="MKGKIIPEKNKIKNLINQERDAIALWIKDGMRLPDIYRLLKEKH
                     PELTFSQNGFVFSLRRDASELHENALFNRSTVAVFMQKQHSEMALMVNSGCLLKNVHE
                     ALFSHISYSVFLRYIVKLYPDLHYQAKLNRKGAVCGELHHEENASVYQNDEVC"
     gene            complement(767..1048)
                     /locus_tag="ACHJ12_005042"
     CDS             complement(767..1048)
                     /locus_tag="ACHJ12_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992672.1"
                     /translation="MKTNKKGTKWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(1106..1498)
                     /locus_tag="ACHJ12_005043"
     CDS             complement(1106..1498)
                     /locus_tag="ACHJ12_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992673.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCVMVPRISLPSL
                     KLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTIQGEGRHRFKADLSQNGES
                     DIASNVLNHWLHRIHKTDIIVLSVVLTE"
     gene            2114..2341
                     /locus_tag="ACHJ12_005044"
     CDS             2114..2341
                     /locus_tag="ACHJ12_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFO4992674.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
BASE COUNT          799 a          478 c          582 g          880 t
ORIGIN      
        1 tggcagcctc atgtatttat gcggagatta tttcatcgga tattatttta tccaggaatg
       61 ttgttttgtt aaagtgagta agtcttatag attatgtttc tttactgaat tccgggtatg
      121 tagacaaagc tggttctgta tatgtgaact tctttgttgt tcctgaggga gataatatga
      181 aaggtaagat tataccagaa aaaaataaga taaaaaactt aatcaaccag gagcgtgacg
      241 ccattgcact ctggattaag gatggaatga gactgccaga tatttacaga ttactgaagg
      301 aaaaacaccc ggaactgacg ttttcacaga atggttttgt attttcactg agacgtgatg
      361 caagtgagtt acatgaaaat gctcttttta atcgcagtac tgttgctgta tttatgcaga
      421 aacaacattc tgagatggct ctcatggtta attccggttg tttgctgaaa aatgtgcatg
      481 aggcgttatt cagtcatata tcttactccg tgtttcttcg atatattgtg aagttatatc
      541 cagatttaca ctatcaggca aaattaaaca ggaaaggggc tgtctgtggt gaattacatc
      601 atgaggagaa tgcttctgtt tatcagaatg acgaggtttg ttaaaggtgt tttataaaaa
      661 tagtggggta gtgctggcac agagatatct tacaggcatt ctcccgcaaa aaatgaaccc
      721 gttgcttgtt atagtgcctt gcttaatgca ttgtctgaat ccggagttac tttttaataa
      781 ttctgttaat ggttattagc tcacctaata tttcgaagtt atcaggtggg cagttaatcc
      841 atttgttaga gccagcgatg atttccctgt cccagcgttt tattctttcg ccattttttt
      901 ctatgcatat catgtaattt gatggggtca ttaattcttt agatactgaa agacattccc
      961 gaaatgatgg tatttcaagc aaaacttccg cgcctgaatt ttcacggtaa aaaatgtgcc
     1021 attttgtgcc ttttttatta gttttcacgt gcgtatgctc ataggataaa tataaaacct
     1081 tgtaacattc aggaggtaac aggatttatt ctgtaagaac aacagacaga acaatgatat
     1141 ctgttttatg tatacgatgt agccagtgat ttagtacatt gctggctata tcactttccc
     1201 cattttgtga taagtctgct ttgaaacgat ggcgtccctc cccctgtatt gtgaaagttg
     1261 ccacagtttt ttctctatac ccctgaacat atccgtcgtg ccttatataa tcgactccat
     1321 tgagaagcag tcgaataatt atttctgatt gcaacggagt taatttcagt gatggtaaag
     1381 atatccgtgg caccataacg catttttcat catcagtata gtggtatgcg tgaacatata
     1441 tggtggcatt ttgcctgatg ttgttaaggc ttttcagcag gtctacagtc aactccatcg
     1501 cggtatcctt caggttgtta tcattcgttg atgatgcgat ctgttgaagg cgttggcgac
     1561 aattaattct ttttctcttc tgaaaaattt acggaataca aaaaagatct gtttttaact
     1621 gattgttact ttctgaagat attctctgct ccatttgctt tccgggtaag acgtatggct
     1681 ggttatggta tggtcggact gcagccacag ggggatgact gtaatacctc tgatcaccac
     1741 agtcagtcat ctgtaaatgt taaatacctc acagttcagt cagtggatta ttgtcggtct
     1801 tacgggatat ttgttaaaaa aatactgcat ggtgaatccc cctgagcggc ggggcatatc
     1861 agtatcgtag gtgtttggtc acatccctta tctgtgcggg ttcaggtgct gatactgaac
     1921 tcaccgggag gcacccggca ccatgtacat aacactccat tacgctacac tcgcccctct
     1981 ccggaggggt tttctctgca ggatgtgaat atgagatgct gcattattca ctgcgaatat
     2041 tgatggtgag aatctttaat aatattaatg tttatatgta ttgttacagt gttgatgaaa
     2101 ggagataaaa acaatgttcc cggagtacag agaactgatt tcagcattga aaggaaatca
     2161 taaacgcttt gattctttat ttgaaaagca taataaactt gatcacgaaa taaaaagaat
     2221 tgaagatgaa tttggttcag cgtattcgac gaaagtggtc aggctgaagc ttgaaaaact
     2281 tgagatcaaa agagaaatcc agaaaatcct tgaagatgag tcagaaaagg gcgaacggta
     2341 atcataaacg gtatattcaa agacgtgttt tctctgtttt atgaaaaagg caccgggggc
     2401 tccacggtgc ctttcagcct gtgtataacg aaaaggagat cctgttcagc gaaccagata
     2461 gttaaaaaaa gaatctttac gacggtatca aagttttcgt gcaaaaaaca tgatcaatat
     2521 taaattccag tacatccata tccggcataa acatttccgt ctggcagata cagatatgcc
     2581 acgtaacgta tccgctgttt ccggctgtgc cggaagagaa taaaagtccg tagcttgttt
     2641 tgtttcactg aaggtaaact ttttgtacga taaattccgg gaatatgaag taaaaatcaa
     2701 aacaattccg ggggagatat gcaattatta tattctgct
//
LOCUS       DAZFFI010000248         2737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFFI010000248 DAZFFI010000000
VERSION     DAZFFI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.250"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(255..959)
                     /locus_tag="ACHJ12_005045"
     CDS             complement(255..959)
                     /locus_tag="ACHJ12_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFO4992675.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(970..2661)
                     /locus_tag="ACHJ12_005046"
     CDS             complement(970..2661)
                     /locus_tag="ACHJ12_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO4992676.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
BASE COUNT          603 a          876 c          598 g          660 t
ORIGIN      
        1 ttggtaacta aacaaggtgg attccgatat tagagtcttg aagtgatcac ccaactacgg
       61 ctacactgaa aggacagtat tcggtatctg cgctccacca agccagttac cacagtcgat
      121 tagaagaggt cgaagacttc taatcggacg gaaaaagccg ccgcaaggcg gctttttccg
      181 tttacaagcc gaagattacg cgcagcttaa aaggatctca agaagatcat tttaattttg
      241 ctgatcgttg ctccctacca gtgaccatac ggatagggct tttccttgat aaaatcgcgg
      301 ataaatggat cgtaaccatt agccttgatg taactgaaat caatctggtt gggaacgttt
      361 ttgcatatca gcatatcatc tcccgtcaga gtgaactgcc gggcaggatg ttggagtaca
      421 acaggctcgc cattatcctc cagccagatc cgcacggccc ctcccggtgc caatccgata
      481 atcatattgt cccgataata cgtttttccc ggcttatagg tatcaggata ggcagtcaac
      541 atctgctgcc acgtatcccg gctgaaccag atctccgtct catatgcctt tttatcgata
      601 accgaatccc aacacaccgt catcctgacc ggcagtgcct tgccaatgtt aaaactggct
      661 gaaaaactgc ctacgcgctg gctccatgta tccacactac tctgacttgg tctggctgca
      721 tcaagctgcc gaaatatgct ttcccgtaca tcaccgtcct ccagatacac cattgtcacc
      781 agtgctggca ggtttttcgg gtaaaaaaaa ttaaactgcc agtggtcata aggcagcttc
      841 tgcccccatt gatccatacc gtcgcccaac agcgtcttca cgccagccct acggtcatgg
      901 cagccgacag ccagcattac gcccactatc acgcccacca tcagcggtaa tcgtctcatt
      961 cctgcctcct cagaacagtc cgtccagcgc atccgcgttt acccgcgccc gctcttccca
     1021 ctgctgctgg tcgtccatcc gccacaccgt ccgcacccag cctttgcccg gtgccatcgg
     1081 ccacacgaac gagcgttcgt cagtcggagc atagttattt cgcagaaaaa cttctcccga
     1141 ggacggactc acccacaggc tcaagtctga gtcgatgttc cagttcgccg agcaatgcag
     1201 gtaacgcccg atggtccgca tatcctccgc cgtgaagccc tcaggctccg cgccacgccg
     1261 gacagcacgc ccctgagcta tggctttgtc acacagggga agcagttctg acggcaacgc
     1321 cagctctttg ttactgctga gtatcggagt aaacacagcc ccggcgtctt gtgccgcatc
     1381 cagcatcacc cgcaaactca ccttctccca gtcgttcggc acatccctgc gcgtcataaa
     1441 cactgccgcg cccactttct tctcaaaaat agacgcacgc gccttgttct gattgaccag
     1501 tggccacgaa atcattttcg tctccacctc accgtgcggc atcaggtact tcagcgccgg
     1561 cagtgagtac agcacctgac gcatcttctc cgccttacgg tacacctccg tctgcatttc
     1621 atccgcgctg tcgcgcgtca cctcaaaatc cggcatcgtc agcagggaga tttcatcctc
     1681 tcctgcgtta tagcccccgc caatgtccga gtgcgccccc ggcagcgcca gctccggcca
     1741 catccccgcg atgctgttca ggctgaagtt ataccgacac tcatgcatcg cgctaatctg
     1801 aaacaccttc tgcgccactg aaggacgcag ttcgagattc accccgggat tgctgcgtcc
     1861 gttaacgtcg tagaagttca acaggctccc gatagccgcc actgtgtcaa acagccctaa
     1921 aaagcgcacc tctcccgcca gtttcccgtg gtgcccggtc aggtctgtcc ctttgtctat
     1981 tgccgccgct atcgccgcat cctggttcat cacccggttg gcaaaatgcc gtgcagccgc
     2041 cgcgccgcgg ctgaatccga acacgtcaaa ctgcaccttc gcaatcgcca ctttgtcacg
     2101 attaagtttg ataaagcggg tcagttctgc gctgatcttc tccagtgctt catcggtctt
     2161 tgtcaccacc ccttcaaact gtcggataag gctgctgccc agcgctttac ccagtatcga
     2221 atcactttct ccgtctgccg tcccgatgcc gctgatatag gctttgacct gcgcctcagt
     2281 ctcgctgtcg ttaagttcat cgctttgctg ataaagatcg aacagccggg aaatattagt
     2341 gtggccgccc tgccagcttg cattacccag cccagctctg gcgcgctttt catacttctc
     2401 acatgcccta gcccgctcct cgtcaaccag attggcacag tgggcatatg ccagccgtag
     2461 atcgtctgcg ttcgcccggt tattccccgt cccgtcaaag aacaccccca ccgtcagcgt
     2521 tatccccctg accggcaccc gccgacactg gatggtttta aaactgttgt gtatgctggt
     2581 tcgatgggta ctgtccccaa taatttcata ccggtaataa caggtatcgc ccggcacatt
     2641 tttttcatct tcttcgacca taaaaatcct tatttatctg aaagataaaa tcagtagcaa
     2701 aatccttcgg gactttttta catctgcctg ttagacg
//
LOCUS       DAZFFI010000249         2714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFFI010000249 DAZFFI010000000
VERSION     DAZFFI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.251"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1838)
                     /locus_tag="ACHJ12_005047"
     CDS             complement(<1..1838)
                     /locus_tag="ACHJ12_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO4992677.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSN"
     gene            complement(1882..2565)
                     /locus_tag="ACHJ12_005048"
     CDS             complement(1882..2565)
                     /locus_tag="ACHJ12_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4992678.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          640 a          636 c          592 g          846 t
ORIGIN      
        1 atattggaag tgttattggc gatattggtg gtattcgtcg ccacagcatc gttggtggtt
       61 ttcagctgag agccgttaac agcatcagtg ctgtcagccg tcaggtcggc atctttaacg
      121 ttggtgattt tgctggtggt atcctggccg tgagctgcac tgaatgcctt tttagtttcg
      181 ttccagagca gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg
      241 gtggtattgg tggcaatatt tgccgtattc gtggcaatgt tgctggtatt agtagcgata
      301 ttggcggtat tggcttcgac atcgtcattg gtagctttca gttgggaacc gttaaccgca
      361 tccttactgg aggctgaaat cgtgccatcc gcaacatcgg tgattacgct ggcagttggg
      421 ctactagtac catgagcagc gctgtatttg cctttggttt ggtcccattg cagggtgttt
      481 tcatcaagta cagcgagcgc agcccctacg ttatttttgc taccattttt taaattgtag
      541 gttggtgctg taacagtacc gtcatcaaca tctactgcag cccctcctcc aagccttttt
      601 gctaccgagt cgctaatggc ataaagctgt gaaccattaa tggcatcgta actgtcagac
      661 ttgatcgcgc catttttcac attaacgatc ttgcgtttta atgaactatt gccgacagag
      721 acggtattgt ctaaatccgc aatggaatct gcgcccagcg cgacagagtt aataccgttg
      781 gcactgctat tactaccaag cgcaagactc ttatcaccag ttgcattact ggctgtgcct
      841 aaggcaatgg tgtttaagcc tctcgactgg ctaccattac ccagagcgat ggcatccacg
      901 ccatcagctt tgttgccctg gcctatggcg attgcattat ctgccagagc ctggctgcca
      961 tttcctaatg cgatagtatt gactttatcg gcaaggcttc cgaaccccat tgccatgcta
     1021 ttagtccctt ttgccttcgc attattgcca atagcagttg cattttcagc ggcttcggtc
     1081 tcagcaccta tcgcaatagc gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg
     1141 ctgtcagtac cgctagctac actgctacgc cccagggcaa ttgcgttttc ttcagatgcg
     1201 acgctacttg ctcccagcgc cattgaatac gcttttgacg cattggcggt gtcgccaaga
     1261 gcaatactca taatctcagt agctttagtg gcatttccca gggcaatggc gtttgctcct
     1321 tccgctttgg aagagtcccc catagcaata gacaatttac ccaatgcttt tgaataacgg
     1381 ccgagggcca ttgagtattg gcccaatgaa tacgaagagg cacccagtgc tatacttcta
     1441 tcgccttctg atattgcact aaccccaaag gccattgatg caccgccaat cgcatgggtt
     1501 tttgacccga tggcagagct atattggcct tcagctatag cgtcataacc cacagcagta
     1561 ctggaaccac tggtgttcat aaaagtattt gctttcgccc ctttgcctat agcaacccag
     1621 ccgtcgccag ctgatccact accgtaatca acaccctgac cgttgtcatt cccggcattc
     1681 gccaatgccc caaacgacga caacattcca cccgcaacca gcgcagaaat taacagctta
     1741 ctgcgcccag atttcttgcc acggcttttt gccgtttcgc tggtaacagt ataattccct
     1801 gtcgcagggt tccagataac tttaaatatt ttgttcatta atgatgctcg ctattccttg
     1861 ttataagcgt actaactgca tttatttttt gcttcttttc tgccgatttt cagcagaggg
     1921 aagattgtcg aactgaatat tttccatgtt gatggctaac ttcagatatc ccaggttatt
     1981 gggcgataca cccttcaggg tcaaattaat gtcttgccat tgacctggta ataatttcaa
     2041 tgtggatttc attgatgtgt tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc
     2101 gacaggtttt tcaccacccc atgtcgcatt aaaaccaatg tttgagatag gtgtacttaa
     2161 taagttgacc agtgagatat gaataacact accattctct gtggcgtcaa tggataacac
     2221 tcgacccgct aaagccaccg aaccgttgtt caggatgaaa tacttcgttt ttgctgggtt
     2281 aaactcggta aaaataaaag tattttgttg gtctttaacg gatttaatta cgtgagttaa
     2341 agtctttgta ctgtttatta tgccagtttg ttgtttttga atagtttcga gttgactctt
     2401 tagctgtgct tgttgttttt caagtagcac aattttatta ttatgttctt cttgttgttt
     2461 cagtaaacta ctttctggct tgccttcttc aatgtagtca catcccgtta atgcgcagag
     2521 aactgaaaca gtgacagcac tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt
     2581 tcctccgacc agcgatcaat gccggtagag ttgtccattg tattattggt gagctatgtt
     2641 gctttacatt aatgacagtg atgtgtgtcg attaaatatt gttttaagca ttcatcataa
     2701 aaatgtatca ttcg
//
LOCUS       DAZFFI010000250         2676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFFI010000250 DAZFFI010000000
VERSION     DAZFFI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.252"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1491
                     /gene="tssI"
                     /locus_tag="ACHJ12_005049"
     CDS             <1..1491
                     /gene="tssI"
                     /locus_tag="ACHJ12_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO4992679.1"
                     /translation="AQIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYP
                     GRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVV
                     ASDLHGEQPQAVPGRRGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAG
                     EEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQV
                     YIHAQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVE
                     TVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMV
                     GMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGT
                     HIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1567..>2676
                     /locus_tag="ACHJ12_005050"
     CDS             1567..>2676
                     /locus_tag="ACHJ12_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4992680.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVLKQHKGHPLA
                     RLWRALPEAVRLSPHTYMMAVSTTGQWLILGWPERVPEADEVPPPEPPAYRVLTGVVD
                     GFGRSLIFHREAAGELAGEITGVTDGAGRRFHLALSTQAQRAEAFRKQRVTSLSSPAG
                     PRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGE
                     LRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          617 a          716 c          861 g          482 t
ORIGIN      
        1 gcacaaatcc gtccttcttc cgtggtgaca aaagactaca cctttaaacg tcccggctgg
       61 gcagggcgtt ttgaccagga gggccagcac caggactacc agcgcacgca gtatgaagtg
      121 tatgactacc ccggacgttt caagggcgcc cacgggcaga actttgcccg ctggcagatg
      181 gacggctggc gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg
      241 ccgggacggc gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag
      301 gtggtggcga gcgatcttca cggcgaacag ccgcaggcgg taccgggacg caggggttca
      361 ggcaccacgc tggataacca ttttgcggta ataccggcag acagaacatg gcgaccacag
      421 ccgttgctga aaccgctggt ggacggcccg cagagcgccg tcgtgacggg accggcaggc
      481 gaggaaatct tctgtgatga acatggccgc gtgcgggtga aatttaactg ggaccgttat
      541 aacccgtcaa accaggacag ttcatgctgg atccgtgtgg cacaggcgtg ggcaggcacc
      601 ggattcggta acctggcgat accgcgtgtg ggtcaggagg tgattgtgga cttcctcaac
      661 ggcgatccgg accagccgat cattatgggg cgcacctacc accaggaaaa ccgcacaccc
      721 ggcagcctgc cgggaacaaa gacgcagatg accattcgtt cgaaaaccta taagggcagc
      781 gggtttaatg aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg
      841 cagaagaaca tgaacaccga ggtgctgaat aaccgcacca ctgatgtgat aaacaaccat
      901 gccgaaaaaa taggtaacaa ccaggcgatc accgttacca ataaccagat ccagaacatt
      961 ggcgttaatc agatacagac ggttggtgtc aaccaggtgg aaacggtggg cagtaaccag
     1021 attatcaaag tgggatcaaa ccaggttgaa aaggtgggga tcattcgtgc gctgacggtg
     1081 ggtgtagctt accagacgac ggtaggcggc attatgaata cctcggtggc gttgttgcag
     1141 tcctcacagg tagggctgca taaatcactg atggtgggaa tgggctacag cgtcaatgtg
     1201 gggaataacg tcaccttctc ggtgggcaag acgatgaagg aaaacaccgg acaaacagca
     1261 gtttattctg ccggtgagca tcttgaactc tgctgtggta aggcaaggct ggtgctgacg
     1321 aaggacggaa gcatatttct caacggtacg cacattcatc tggaagggga gtcggatgtg
     1381 aacggtgatg cgccagtgat taactggaac tgtggtgcca cacaacctgt accggatgcg
     1441 cctgtgccga aagatttacc ccccggaatg ccggatatgc ggcaattttg agtcaggtgc
     1501 aaaaaattaa aacggaactg gtgcagtgac cagatgaacc atattaaaag cagaaggaat
     1561 tgatttatgg gcggcggaaa accggcggcg cggcagggtg acatgacccg caagggactg
     1621 gatattgtgc agggttcagc aggggtgctg ataggtgcgc caaccggtgt ggcctgctcg
     1681 gtgtgtccca caaaaaagga cagtccgaac tatggcagcc cggtgaaccc gctactcggt
     1741 gcgaaggttc tgcctgttga aaccgacctg gcactgcccg gcccgctgcc gtttattctt
     1801 ttccgcgcct acagcagcta ccggaccagg acgcccgcgc cagtgggcgt gtttggtccc
     1861 ggctggaaag cgcccttcga tatccgctta cagatacgcg atgaaggcct gatactcaac
     1921 gacagcggtg gtaggagcat tcactttgag ccgttgtttc ccggcgagat aagctacagc
     1981 cgcagcgagt cgttctggct ggcaaggggc ggagtgctga aacaacacaa aggccatccg
     2041 cttgcgcggc tctggcgggc gttgccggaa gcggtccgtc tgagtccaca cacctatatg
     2101 atggctgtca gcacaaccgg gcagtggctg atactcggct ggccggagcg tgtgccggag
     2161 gcggacgagg tgccgccgcc ggagccgccc gcataccggg tgctgacggg cgtggtggac
     2221 ggcttcgggc gctcactgat tttccaccgc gaggccgcag gcgagctggc tggcgaaatc
     2281 accggggtga cggacggcgc gggccgccgt tttcacctgg cactgagcac acaggcgcag
     2341 cgggcggaag ccttccgtaa acagcgcgtc acgtctttat cctcccctgc cggtccccgt
     2401 tctgtttcct cttctcaggt tttccccgac acgctgcccg ccggtacaga gtacggtgcc
     2461 gataacggta tccggctgga ggcggtgtgg ctgacacacg acccggccta cccggatgaa
     2521 cagccgactg cgccgctggc gcgctacacg tacacggcca gcggagaact gcgggcggtg
     2581 tatgaccgca gcgggacgca ggttcgcggg tttacttatg atgcggagca cgccgggcgg
     2641 atggtggcac accattatgc ggggaggccg gagagc
//
LOCUS       DAZFFI010000251         2675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFFI010000251 DAZFFI010000000
VERSION     DAZFFI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.253"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..1614
                     /locus_tag="ACHJ12_005051"
     CDS             64..1614
                     /locus_tag="ACHJ12_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO4992681.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPREFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1640..2092
                     /locus_tag="ACHJ12_005052"
     CDS             1640..2092
                     /locus_tag="ACHJ12_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO4992682.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2245..>2675
                     /locus_tag="ACHJ12_005053"
     CDS             2245..>2675
                     /locus_tag="ACHJ12_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4992683.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQR"
BASE COUNT          789 a          490 c          635 g          761 t
ORIGIN      
        1 ggacacttac ttgctctttt atgaacactc ttttatgaaa attgaaacgg agaatactgc
       61 cagatgagtc atcagtgttc actttctgaa ctgaatgaaa acctggtgcc tttcactgcc
      121 aggcagatca aggcctcatt aatctggtgt gcagaggatg tcagaaatcc agacgagctg
      181 caaaatgcct gcagttatat tatcgatcct ggcagtacag cttctgccaa agtgttccat
      241 gcagagcgct atggtggcag tggtattcag cgtaatggag gtggtgcacg ttgtgggttt
      301 gatggtaact accaggttaa aggaatagga agtaatccgt tggttggcga aggtactgac
      361 gaacgtcatt ctaatggtgc actcggcgct gttcatgcaa tatatgaggc tttgtgggga
      421 gaagtactgg ctcaaatatt accttatagt gctgtgcggg ttcgggcggt tttacttaca
      481 gatctctata ctgaaaaggc atttgagcgc tccggtagga aatcacgaag agccctgttg
      541 gtacgtgagc ctgttgttcg tccggcgcat tttgaacggg caccttactt ccaagtaaaa
      601 ccggagtatt ccagtctgct aattcacgat gcctgtcggg ttagatctgt gatccacaag
      661 ctgccaggat atctacctgt accaccggaa gaaattgatg ctgaagcacg aactgatccc
      721 cggatttatt gcattgaggg gttatgtgaa ctggcacgtc gtgaggcctg gcaaatggca
      781 ttttgtcgaa cacgtttcct gagattgaca acttctcctt ctaatattgc aatggatggc
      841 agattaatgg attttaacgg actcagttgt ctgtttccgg gagattcccc agctgatttt
      901 gggtataaac taagattagc tgaactggca aaagaaccga tggtacttat gcaagggctg
      961 tctgatctct gcttgtatat cggaaaatat atgtttgacc ctgacttcac tcttgcagcc
     1021 cgtttgaagg ttgaggagat atttcagaaa actttccatg aagcatgtta ttactgttat
     1081 ctagaactgt tgggtattcc tagagaattt ataacacaaa aagagatacc tgatatattg
     1141 aaagaactgg ttaacagttt tgttgcatta ctcaatcaat actgcgagaa atcacatgcc
     1201 caagatattg tcaatcagga tggttcacca ttgcaaaagt tggttgtgac gctaatccat
     1261 cataggcata atcaaaagca ggcactgaat agtagcatca agaatgatgt ttatttcacc
     1321 gttgcacaac agtgtttttc ccaagccatc cactggctga cgcaaggcag taccagacgt
     1381 cagataaatg cctcattact cctgaaagaa attgaacatc ataccatgaa aaggctgcaa
     1441 cccagggaag agctgaggaa agaaaatatg tgcgaaaaaa ttgccatcct gctggataat
     1501 catggcgatg atcccctttt tttacaagaa gcaatttctg atatgaaaaa ttttatgctt
     1561 aagttttcca gagatgcatt tggatatctt gaaccgataa gaaacacagt gtaaaaaata
     1621 tataagggaa atagtaatca tgtcttatat aagggaaacc atcagaggaa aagatgaatg
     1681 gactgtttat gaacagatcg gttttgcggt cagttgtatg ctctacaatc gtaattacag
     1741 tctgtatccg gtgttaacca ttcaatactg gactgaatat gcgatacagc ataatcagat
     1801 taaattcctg tttgattcac gaggttttcc actggcgtat attacctggg catatcttga
     1861 ggctgataca gaagcgcgcc tgctcaggga tccagaattc aggttgcatc cgtctgaatg
     1921 gaatgaagat ggaaggattt ggattctgga tttctgttgt aaaccaggct ttggtcgaaa
     1981 agttattgac tatctcatac agcttcagcc atggggagaa ggagaagtac gatggttaag
     2041 caggcgaaag aaaattgtga catacatccc tgagcggctg cataaaacgt agtacctctg
     2101 aagatacaga gataattgta aattacgggg taaatgcatc gctgatacta ttttgacagg
     2161 actctgtatt ttctggcttt gattatagca tttcgcttaa ccagtgaagg tattacagga
     2221 aaagtgtgag taagaggagc tattttgttt cgtcaggatg ctttagaaaa cagaaaaatg
     2281 aagtggcagg gacgggcaat attacttccc ggaataccac tatggttaat catgctggga
     2341 agcatagtgt ttattacggc atttctgatg ttcattattg ttggtaccta tagccgccgt
     2401 gttaatgtca gtggtgaggt cacaacctgg ccaagagctg tcaatatata ttcaggtgta
     2461 cagggatttg ttgtcaggca gtttgttcat gaaggacagt tgataaaaaa aggggaccct
     2521 gtttatctga ttgacatcag taaaagtaca cgtagtggta ttgtcactga taatcatcgt
     2581 cgggatatag aaaaccagct ggttcgtgtg gacaacatta tttcccgtct ggaagaaagt
     2641 aaaaaaataa cgctagatac cctggaaaaa caacg
//
LOCUS       DAZFFI010000252         2668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFFI010000252 DAZFFI010000000
VERSION     DAZFFI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.254"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(190..408)
                     /gene="ymgF"
                     /locus_tag="ACHJ12_005054"
     CDS             complement(190..408)
                     /gene="ymgF"
                     /locus_tag="ACHJ12_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO4992684.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(540..2063)
                     /locus_tag="ACHJ12_005055"
     CDS             complement(540..2063)
                     /locus_tag="ACHJ12_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4992685.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2395..2643)
                     /gene="ymgC"
                     /locus_tag="ACHJ12_005056"
     CDS             complement(2395..2643)
                     /gene="ymgC"
                     /locus_tag="ACHJ12_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO4992686.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          828 a          579 c          542 g          719 t
ORIGIN      
        1 taacaaataa ctcttcgaca tcacaatcat tgatagttgc aataaaaatt atttcaatga
       61 catataaaaa caactgtgat aacaccaggg ttgacaacta tttgcgcaat aattccttta
      121 cttcaatgat ccaggcattc actgccagca acccattcag ccgcatactg acagtgagaa
      181 actgaaagat tattgctgat ccctgtggct gacgagtctt tcccttaatc gattaaccag
      241 attgatcgcc agccaaacaa gggcattaag caaatttatc gtcacaagca cttctcccag
      301 cccggtactg ggttttgcaa taaggatgac caggagggtg atcagcataa atgcaatgga
      361 aaagatgata tagagagtga aataatccag attattacta ttgttcatgc tactaatggc
      421 aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat agtaacaaga
      481 ggcaacgagc taattagctg tctataatta caccgcacta tggccttact gatcaatttt
      541 cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag gtcgatgtat
      601 gtaacaggtt tatagaaata ataaccctgc tgaaatgtga tattattttg gttcagatag
      661 tcaagttgtt ctttcgtttc gacaccttca gcgacgatac tgatcgaaag tttacgcgcc
      721 agatccaata cacaatccag aattcgggtt gattctgggt ctgcgtttac gcggccaacg
      781 aaactgtgat caattttgat ataatcaata tgcaggtcat gaagataaga aagccctgag
      841 tagccagtac cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag aatgttcaac
      901 cgctgaacca gactttcatc aacattcaat ggctcacgct cggtgacttc cagaacaagg
      961 tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac actcgtcgac aaacgtcggc
     1021 gaaataatat gcgaagcgct aaaattaatt ccaatatgaa aaccttccgg caatttactc
     1081 gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc tttgcgtaag cggaacgatt
     1141 aatccggatt tttcagcaag tggaataaat gccgcgggtg atatatatcc accgtgaggt
     1201 tgtttccagc gggctaacac ctcaactccc cgcaatgtcc cttcccgacc atttaccaca
     1261 ggttggtaaa aagggacgat ctccccatta tttattgccc gtcgcaggat ctcttcaggc
     1321 gttgcgcttt tattgaaata tctatcaagc aggaaggcag cggcgcaggc gattaacaga
     1381 ataaatatta aaatcccaaa gcctttatcg ataagtctgt agaagctaaa gagtggcggc
     1441 gggttaaaga taatagtgaa atgatatttt gtggagtgaa ccttctcggt atagggataa
     1501 tgtccattaa gcgtcatcac atcacccgtt ggtccaataa tgtcatccgc cacgcgtaat
     1561 acatacctta cccctttcaa gggtacatta agtgccccac gaatatgctg atcgcttatc
     1621 gtaaccaaaa tgcgcgtatc tgcaaattgg ttctgataga gcaggatagg taatctatta
     1681 acggtgtcga ttgctggagc caacagtaaa ttactgtccg ggaaaacagg aatacgagac
     1741 aacaggaccc gattcccagg cagggatgta caccagacta ttccctgttt gagaatgatg
     1801 attgtgcgca ggtgaggttt cagagctgct tcagtgccaa gctgatattg ctcctcgagg
     1861 ttgcattcct tcccggcaat gttaaccgct gtttgagtcg cttgtgacgc ttcttcaagt
     1921 atatgattga tattgttggc ggcatatctc gctcccgcca ggtactctgc ttttgcggaa
     1981 taccagagct ggatgttcaa aatagcgaca ccggtaataa acaagcatat cgcaacgagg
     2041 atgggtatga gtgtattgcg catttgcttg tcccggccaa agggtttaat acatgaaaat
     2101 gccattcatt cgctcgcgct ggcatctaca gagagcatgg tagagagcat aagaaatatc
     2161 cacgccgaag ataaaatttt ctccatcttg atctaaccca ttaattattg agcataatgt
     2221 aggcagtaca aaataagtta ggcgggatat caggcgtcaa gaatggagag cgagaactct
     2281 ccattcttga cacctgatat tgcggacata ataagaaagc ataacgcctg aaatgctcac
     2341 tttgcatcag catggtgata cagctgatgt ttattctaaa accttactca agttctaaga
     2401 gagcacggat tccctgtcat taataatgat atccgttagt gtagtcttat gcatttcaat
     2461 gtactgcaat atttgcagat agagatcaga aagaatcaca ctggtttcat cttttaaacg
     2521 caagcgtaaa gcctgatcaa taatatgact atcaacatac ccatgcgtca tgaacacctc
     2581 cctctcgaga tttttaaaca aagcacattg aaaaataaat ctctctggga ttgaattatt
     2641 catgtcagcc tcgtagaaat attaaccc
//
LOCUS       DAZFFI010000253         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFFI010000253 DAZFFI010000000
VERSION     DAZFFI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.255"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..830
                     /locus_tag="ACHJ12_005057"
     CDS             <1..830
                     /locus_tag="ACHJ12_005057"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4992687.1"
                     /translation="NYMNKIYALKYSSLTGGLIAVSELSKKVTGKTDRRLMTVSLVLS
                     VTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMID
                     FSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHR
                     FSKLIAEATPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTV
                     GTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKMVSGWSHPA"
     gene            793..1497
                     /locus_tag="ACHJ12_005058"
     CDS             793..1497
                     /locus_tag="ACHJ12_005058"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4992688.1"
                     /translation="MKKWSLAGVTLHNYGVNGYRNNWLLLPEDYIRNIIVADFDPIIS
                     FNKNSKEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIIDLKD
                     NVNQGAGYLQFADDYTVTTSNDSSWSGGGIIVNYGTTVKWGINGVSGDDLHKVGDGTL
                     IINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPDNV
                     SWGFRGGIFRYKRKRH"
     gene            1442..>2655
                     /locus_tag="ACHJ12_005059"
     CDS             1442..>2655
                     /locus_tag="ACHJ12_005059"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4992689.1"
                     /translation="MFPGDSGGGYLDINGKDTEFSRLQAVDYGAAIINSSTDKSLLTL
                     NLSPLKKDEIAVSVKALDMNAIFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMG
                     SLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDIL
                     TLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFS
                     LSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHIT
                     LEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGL
                     FTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGD
                     IISDKQATISFGT"
BASE COUNT          805 a          499 c          629 g          722 t
ORIGIN      
        1 ggaattatat gaataaaata tacgcgctta aatatagctc ccttactggt gggcttatag
       61 ctgtgtcaga attaagtaag aaggtcacag gaaaaaccga tagaagatta atgacggttt
      121 ccctggtatt atcagtgact ctttctgctt taccgggtaa agcatcaacg gtcagtgcag
      181 aaataccata tcagactttt cgtgactttg ctgaaaataa aggtgtgttt acccccggag
      241 tgacaggaat tgaaataaaa gataataatg gaaatgcagt tggtacgctt gatgtgccaa
      301 tgattgactt cagcagcgtg tcacggcggg gctcactgac cctgttgtcg cagggatatg
      361 gtgtatcagc aaaacacgga gaccttggtg atgtaaacaa tgccagcttt ggctatgata
      421 aaaataatta tactgtagta aagaacaaca aacattcggg ccttgatttt tctctgcatc
      481 ggttcagcaa actcatcgca gaggctacac ctgctgatat aaatatatct ggacaactta
      541 gtgattcgtc acagtatact gctttttaca gagcaggcgc tggcacacag tatattaaag
      601 aacgctcagg taaacagact catatcccgg gaacattttt aactggtgga acagtaggca
      661 ctccatggta ttcaggaaat aatttaatat caagctctcc aggtgacacc tataataaat
      721 cacagggacc attagccagt tatgggcaga tgggtgacag cgggtcgcca ttatttgctt
      781 atgattctct cagtgaaaaa atggtctctg gctggagtca ccctgcataa ctatggggtg
      841 aacggttaca gaaataactg gttgttatta cctgaggatt atatcagaaa tattattgta
      901 gccgatttcg atcccattat ttcatttaat aaaaacagta aagaacatat gtcatggaca
      961 tatgatgcag ccaaaggcgt gggccgtatt cagcaggatg accaacagtt tgtgatgcat
     1021 ggtaacctga atggtaatct gaatgcaggt aaaaatcttt attttacagg cgaaaatggc
     1081 attattgatt taaaagataa tgttaatcag ggggccggat atcttcagtt tgctgatgat
     1141 tatacagtaa caacgagcaa tgacagttca tggtccggag gaggcattat tgtcaattat
     1201 ggtacaactg taaaatgggg tataaacggg gtttccggag atgaccttca taaagttggg
     1261 gacggaacgc ttattattaa tggaaccggg aaaaatgaag ggggactaaa gattggggct
     1321 ggtactgtaa tccttgaaca aaaagcgaaa aacaacgatt caacggcttt ttcttccatt
     1381 aacatcagtg ggggaaactc gcgcgttaaa ctttcaggag ataatcagat tatacctgat
     1441 aatgtttcct ggggattccg gggggggata tttagatata aacggaaaag acactgagtt
     1501 ctcccggtta caggctgttg attatggggc tgcaattatt aacagcagca cagacaagtc
     1561 attattgacg ttaaatctgt caccattgaa aaaagatgaa atagctgtaa gcgtgaaggc
     1621 gttagatatg aacgccatct ttcagggggg acacgggaca gccggagatt tgtataaaac
     1681 aactttttac ggtccgacac agtattatct gctgaaaaag cccaaatttg gcagtgtgct
     1741 gatggggtca ctgaaaaaca ccagcgaatg gcaatttgca ggaacagacc tgaatcaggc
     1801 cgttgatatg gcaaagaata ataaactgac atcaagtgca caggcttcat atctttatca
     1861 cggtaagctc ctggggaata tggatattgt cattccggag cttacaggca atgatatttt
     1921 aacgcttgat ggctctgtca gtatatccgg ggatatgtca aaacaggacg gtgctcttat
     1981 cttccaggga cacccggtta ttcatgcagg gcaaactgtt tctgcatcgc agagtgactg
     2041 ggagaacagg gagttctcac tcaacaatct gaatcttaat aatgcggact tcagtctgtc
     2101 ccgtaatgca tttatgaacg ggaatatcag ggccgttaac cagagcactg ttattatcgg
     2161 cggagataca gtctttactg ataaaaatga cggaacaggt aatgatgtca tcagtgttga
     2221 agggaaatct gctgccgcag gaacatcctc ctatacaggg catatcactc tggagcaaaa
     2281 atcagcactg gatatccgcg ataattttcg tggcggggtt acgtctgaag acagtcatat
     2341 caatgtttct tcatcttcag tcctgttctc agatgcatcg tcatttataa acagctccct
     2401 gaatattcat aaaggaggtg cgctgaccgc tcagggaggg ctgtttacaa gtggaagcat
     2461 tgatattggt gacgcttccc ttctgcttac cggtacacca gtgaattcag atgatgctgc
     2521 ttttttaccg accatcaata tggctgatgg cggatttaaa ctgatgtctg attcatcagt
     2581 actgaaagcc agagaccagg catctgttgt tggtgatatt atttctgata aacaggccac
     2641 aatcagcttc ggaac
//
LOCUS       DAZFFI010000254         2637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFFI010000254 DAZFFI010000000
VERSION     DAZFFI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.256"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            240..434
                     /locus_tag="ACHJ12_005060"
     CDS             240..434
                     /locus_tag="ACHJ12_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4992690.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            795..1088
                     /gene="iss"
                     /locus_tag="ACHJ12_005061"
     CDS             795..1088
                     /gene="iss"
                     /locus_tag="ACHJ12_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO4992691.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1179..1361)
                     /gene="rzoD"
                     /locus_tag="ACHJ12_005062"
     CDS             complement(1179..1361)
                     /gene="rzoD"
                     /locus_tag="ACHJ12_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4992692.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1578..2075)
                     /gene="rrrD"
                     /locus_tag="ACHJ12_005063"
     CDS             complement(1578..2075)
                     /gene="rrrD"
                     /locus_tag="ACHJ12_005063"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4992693.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2075..2290)
                     /gene="essD"
                     /locus_tag="ACHJ12_005064"
     CDS             complement(2075..2290)
                     /gene="essD"
                     /locus_tag="ACHJ12_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO4992694.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          756 a          637 c          560 g          684 t
ORIGIN      
        1 aaattttcat aaatagcgaa aacccgcgag gtcgccgccc cgtaacctgt cggatcgccg
       61 gaaaggaccc gcaaaatgat aataattatc atctacatgt cacaacgtgc atctacgcca
      121 tcaaaccacg tcaaataatc aattatgacg caggtatcgt attaattgat ctgcatcaac
      181 ttaacgtaaa aacaacttca gacaatacaa atcagcgaca ctgaatacgg ggcaacctca
      241 tgtcaacgaa gaacagaacc cgcagaacaa caacccgcaa catccgcttt cctaaccaaa
      301 tgattgaaca aattaacatc gctcttgagc aaaaagggtc tgggaatttc tcagcctggg
      361 tcattgaagc ctgccgtcgg agactaacgt cagaaaagag agcatataca tcaatccaaa
      421 gtgatgatgg atgaacatcc cggtttcttc caccatcgca ccggaaaagc gactatgagg
      481 gtaaccctgc gtctgtcagc acagtaaaac ccggtgtgca tcgtttttga ttattcccgc
      541 acactcacgc agaaggaatt ccccgtcggg ctacggtcat ggttaatgcg ggaatacggc
      601 gacgatacag cgcagctaaa agggtaatgg acagatagag cggtttattt cattccacag
      661 gattctgagt gccccccctc ctccaatagg ctgagcatcc acctatatag ttttaatttt
      721 catcaatcca tttaactatc gtttaattgt tgtcacatag gattctgccg tttttaacaa
      781 tgcaggataa taagatgaaa aaaatgttgt tttctgccgc tctggcaatg cttattacag
      841 gatgtgctca acagacgttt actgttggaa acaaaccgac agcagtaaca ccaaaggaaa
      901 ccatcaccca tcatttcttc gtttcgggaa ttggacagga gaaaactgtt gatgcagcca
      961 aaatttgtgg cggcgcagaa aatgttgtta aaacagaaac ccagcaaaca ttcgtaaatg
     1021 gatttctcgg ttttattact ttaggcattt atactccgct ggaagcgcgt gtgtattgct
     1081 cacaataatt gcatgagttg cccatcgata tgggcagctc tatctgcact gctcattaat
     1141 atacttctgg gttccttcca gttgtttttg catagtgatc agcctctctc tgagggtgaa
     1201 ataatcccgt tcagcggtgt ctgccagtcg gggggaggct gcattatcca cgccggaggc
     1261 ggtggtggct tcacgcactg actgacagac tgctttgatg tgcaaccgac gacgaccagc
     1321 ggcaacatca tcacgcagag catcattttc agctttcgca tcagctaact ccttcgtgta
     1381 ttttgcatcg agcgcagcaa catcacgctg acgcatctgc atgtcagtaa ttgccgcgtt
     1441 cgccagcttc agttctctgg catttttgtc gcgctgggct ttgtaggtaa tgacgttatc
     1501 acggtaatga ttaacagccc atgacaggca gacgatgatg cagataacca gagcggagat
     1561 aatcgcggtt actctgttca ttgctgaccc cacaaacaga tttcacgctc aatctcacga
     1621 cgagtcatga gacctttcca ttgcttaccg ccagcatatg tccagcgacg tagctgatca
     1681 catgcgcctt tgatatcgcc ctggtttatt ttgcgaagaa gcgtcgatgt tctgaaattg
     1741 ccagcaccca cgttgtaaac gaatgagtaa agagcgccgc gcattgtttc cggtatatcg
     1801 actttgatgt acgggttaat ttgtctggcg accgtggcaa ggtctttatt caggagggct
     1861 ttgcattctg ctttggtata cgttttaccg agcatgatgt cttttcctgt atgcccgtga
     1921 catacagtcc atacaccaac aatatctttg tatggtatgt agctgacacc ttccagacca
     1981 tcgttaccac ttgggccagt gattaacact gatgctatag caattgctcc gccaccaata
     2041 gcagcagcaa cggcttttcg taatgatgga ggcattattc acctctcgca gccttgcgct
     2101 tatcttcttt aatcttgaaa taaaggtttg tcaggtacgt cagcaggcca aataccaggc
     2161 tacccagcac acctattgct gcccactgtg agggcgtgac tttatcgagc agctgtaaaa
     2221 accagtaacc ggcactacct gctgaggtgc cataggcgac acccgttgtt aacttatcca
     2281 tggatttcat aaccccacct cgcagacaaa gcgggtgtaa attgagggaa tacaacgtat
     2341 cgcaaaaaag cagaaacgta acagactcgg agtcagtgaa taactcaggt attgagttat
     2401 cagctaatat cgagactcaa aaaatggaaa aaccagctcg acggcgggtt taagctgtgt
     2461 gacgaagtaa ccactcttaa cagcataacc aattttttac gtacgtaaac cactgaatga
     2521 tatttatgag aatgctaccg agtgttcaaa acaccaccac aaatacataa gaaaacctca
     2581 acaaataacc aataaataat ttccggcgtt atttttagtt aatttaaatt aaaccac
//
LOCUS       DAZFFI010000255         2633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFFI010000255 DAZFFI010000000
VERSION     DAZFFI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.257"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..457
                     /locus_tag="ACHJ12_005065"
     CDS             122..457
                     /locus_tag="ACHJ12_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4992695.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVLPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECFNNHCILQIC
                     PYGLKQQCP"
     gene            complement(801..998)
                     /locus_tag="ACHJ12_005066"
     CDS             complement(801..998)
                     /locus_tag="ACHJ12_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4992696.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(1225..2046)
                     /locus_tag="ACHJ12_005067"
     CDS             complement(1225..2046)
                     /locus_tag="ACHJ12_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4992697.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFDDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            complement(2043..2417)
                     /locus_tag="ACHJ12_005068"
     CDS             complement(2043..2417)
                     /locus_tag="ACHJ12_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4992698.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(2430..>2633)
                     /locus_tag="ACHJ12_005069"
     CDS             complement(2430..>2633)
                     /locus_tag="ACHJ12_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4992699.1"
                     /translation="ADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEK
                     KYGSQIELIFRFLDHAFATGVLG"
BASE COUNT          703 a          707 c          514 g          709 t
ORIGIN      
        1 acctgtattt aaataaacaa taaacaacat cagaaaaata attctgctct atggtttaca
       61 ttcaaaatta tcatttatac ttttcagaac atcaccagca cagcataaat aaggaggcta
      121 aatgaagtgg attgtgattg atacagttat ccagccatca tgcggaatat ctttttcagc
      181 catatggagt aaaataaaat taataatctg gtatcaatcg gatgttttct tacctcctga
      241 aagtatattt acgttgactc acacaggtat cgtacttaat aacaaagtgc tacctgtaac
      301 catttacaac gttgtaccat tcaataaaag attctgggat ttaatcaaaa acagccagga
      361 atgtcctgca aattcagata acgtattgaa agaatgcttt aataatcatt gcattctgca
      421 aatatgccct tacggactaa aacaacagtg tccataatta gtttactcat atcttacaaa
      481 attcatataa aagcccctcc ggagaggggc taaagccgcg tatctgtatc atcatgcgca
      541 tgatgccggg tgcctcccgg tgagttcagc ccggtgccac caaacccgcg cgtttacttt
      601 cctgggtatc aaagataaca cctaatgcac cagtcgcccc tccgctcagg gggattcacc
      661 atgcctgttt cttttaacaa actccccgca aaacagacaa ctgtcaaccg tctgaattgt
      721 gagacattta aaattttcgg ggcatgactg atacccggct aactacctgg catgtccttt
      781 ttaaccaaag gaaaaagcct tcaggactga aggcgtctgt aacaaccgaa ctgatagtct
      841 gccagccccg ccataacaag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
      901 gtattctgcg caatctcccc gactgtcgcc ggttcggtga cgcttaattc attaaacacc
      961 actctggcgg tttcggtcat atcctgctgt ttcagcatgt ctttttccct tttccggtta
     1021 acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagacc ggtattcacc
     1081 gccgccagcg cgtttactgt actggagtga tttcagtcat aaaaaaaccc gcctggcgac
     1141 gggtgtaaaa agtcttctaa cgtcaggcat aaaacgccca tcgttagggc aaatttacca
     1201 cagattccgg aaaaatcaac cttgttacct ggttaccttt tttaactgcc tctcagccca
     1261 tgcttcctca atatcaaact tgctcaccag cgcatcatag aatttcttaa ctgttttttc
     1321 ccatgacgcg cgtgttatct ggtttgtcac ctcgcatata gcattaaatg cctctgttga
     1381 cggcaatctt tcatagccac gaccaccaca acgctggcag tccctgataa caggcatacc
     1441 ccgtttttca gactcttcac gatgaatggc aacaccgcgc ccacgacaat ctttacaggc
     1501 ggtggaaacc tccccctttc cgccacactc cggacaggca acttttacca cctccctgac
     1561 ttttttccat tcctcccagt aagacggata cacgcctttt gtgcactttg cccacactgg
     1621 cggcttacca tccggatact ggatcttgtt tgtaaaaacc tcgctttcaa taaatttttt
     1681 tccatgacaa caggagcact gttttttgct cgccgcgcta cgggcataat catcaaacgc
     1741 atacgaagcc ataatacgca tcactgccgg ttttatttct gccgggagtt ttcttaacgc
     1801 cgccacgcaa tcacaccgac tgagtgcata ttctgtcagc aattctgttg cccgctctct
     1861 gtcattcata ctaatgccca ttttcccaag gaacgcagaa aaccccatct cagcccgatt
     1921 ctgtgtcatg ccctgcgcgg ccatcacatc agtgatactc agcgcatctt tcgacgttga
     1981 ggccgatgca tcagtcaggc cgggggattt tggggagtag tatttcggta aatcttccag
     2041 tttcattttt tgacctgccc ttcaagcatt atggggtaaa tcttcacccc cagacgtcca
     2101 ccagatactg gctggccacg tacaatattg atttcatcaa actgctcatc gtccattaac
     2161 aaccccgcgt gcgtcagcgc atccagcggt gctttcagaa tattgtccag gtcacgacga
     2221 cgcttatccg gtggctctgc aataattttt attgccagcc gtccggacag gcttaatttc
     2281 aactgctgct ggcgaacaat aagtgccact gcccggcgat aacgctcccc ggattttgat
     2341 acaaaatatg tgctgccacg gcgtcgccag taagtgttca ccgtcggcgg gtaaggtaaa
     2401 accaaatcta tgagcatcag tcacctcttt tacccgagca cgccagtcgc aaaggcgtga
     2461 tcaagaaaac gaaaaattaa ctcaatctga gagccgtact ttttctcaaa cttcagcggg
     2521 tctgcatgaa gttcgttgtg gtgctcccgg cacaacggta gcgtgaaaat atcgtgggcc
     2581 tttgtcccca ttcccccctg accatgacca atcaggtgat gcggatcgtc agc
//
LOCUS       DAZFFI010000256         2625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFFI010000256 DAZFFI010000000
VERSION     DAZFFI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.258"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..385
                     /locus_tag="ACHJ12_005070"
     CDS             <1..385
                     /locus_tag="ACHJ12_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4992700.1"
                     /translation="KYGIPSNPFSLKHHNCLYASSLVGGNCWHFYIRDEITTLEIAKT
                     VNIENSEFLKAVALMGGGIAYLPYPLVQSEILSGKLVSIMNDYIKTRFEVSLWFRKPL
                     SLRCVTFRNFLVERASQLKDTVQFR"
     gene            674..1651
                     /locus_tag="ACHJ12_005071"
                     /pseudo
     CDS             674..1651
                     /locus_tag="ACHJ12_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1697..1960)
                     /locus_tag="ACHJ12_005072"
     CDS             complement(1697..1960)
                     /locus_tag="ACHJ12_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4992701.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            2150..2392
                     /locus_tag="ACHJ12_005073"
     CDS             2150..2392
                     /locus_tag="ACHJ12_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992702.1"
                     /translation="MNNESEKAMWRCKPVDNPWTLDSLLKKTISAVLPASDKVSHQNK
                     NKLRNNEFIKIEHPCSKSESRIDGDYIISGGTEYRL"
     gene            complement(2440..>2625)
                     /locus_tag="ACHJ12_005074"
     CDS             complement(2440..>2625)
                     /locus_tag="ACHJ12_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFO4992703.1"
                     /translation="DLIMNLVMGTMFLVLPAVWLGALSWAGVHIGDGISRGLPNGISE
                     AKGAASSAGSIANRGIK"
BASE COUNT          784 a          590 c          579 g          672 t
ORIGIN      
        1 taaatatgga ataccctcca atccattcag tttaaagcat cataactgtt tatacgcatc
       61 ttctcttgtt ggagggaatt gttggcattt ctatattcgt gatgaaataa caacattgga
      121 aatagctaaa acagtaaaca ttgaaaatag tgagttttta aaagcagttg cactaatggg
      181 tggggggata gcatacttac catatcctct tgttcaatca gaaatactgt caggaaaact
      241 agtatcaata atgaatgatt atattaaaac tcgttttgaa gtaagtctgt ggtttaggaa
      301 gcccctatcc ttgcgttgtg ttacatttag gaatttccta gtagaaagag cctcacaact
      361 caaggatact gtacaattca gataaatcac ttaacttgat gcatcaatca cccagcgacc
      421 agttatgctc atcataatca ttattcaggt gtgagcataa tgagcataac ccagcgatcc
      481 agacaggttc cctgaatctc cagacaacca atatcaatta aataagtgat agtctaaaaa
      541 ctagtcatta gagaacagat gattgatgtc ttagggccgg agaaacgcgg acggcgtacc
      601 acacaggaaa agatcgctat tgttcagtag actggccccc tgaatctcca gacaaccagt
      661 atcacttaaa taagtgatag tcttaatact agtttttaga ctagtcattg gagaacagat
      721 gattgatgtc ttagggccgg agaaacgcag acggcgtacc acacaggaaa agatcgcaat
      781 tgttcagcag agctttgaac cggggatgac ggtctccctc gttgcccggc aacatggtgt
      841 agcagccagc cagttatttc tctggcgtaa gcaataccag gaaggaagtc ttactgctgt
      901 ggccgccgga gaacaggttg ttcctgcctc tgaacttgct gccgccatga agcagattaa
      961 agaactccag cgcctgctcg gcaagaaaac gatggaaaat gaactcctca aagaagccgt
     1021 tgaatatgga cgggcaaaaa agtggatagc gcacgcgccc ttattgcccg gggatgggga
     1081 gtaagtttcg tcagccgtta tctccgtgtg ttgcgtgcgc agttgcacgt cattctcaga
     1141 cgaaccgatg actggaagga tggccgccgc atgatacgga tgtttatctc cgtaataaca
     1201 tggtgtacac catgttatcg gagagctgcc cacgtatggt tatcgtcggc tgtgaaacag
     1261 tacaggacgt catgctggga gcggtagaac accgcttcgg cagcgacctt cctgcgtctc
     1321 cagtggagtg cctgacggat aatagttcat gctatcaggc taatgaaaca cgccagttcg
     1381 cccggatgtt ggaacttgaa ccgaagaaca cggcggtgcg gtatccggag agtaacggca
     1441 tagcagagag cttcgtgaaa acgataaagc gtgactacat cagtatcatg cccaaaccag
     1501 acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc
     1561 cacatagtgc gctgggttat cgctctccac gggaatatct gcggcggcgg gcagtaatgg
     1621 gttaagtgat accagatgtc tggaaatata ggagcaaatc caccacgcat ggagcctgtt
     1681 aagactctca ctctcatcat tctcccttct gagtcccaac gaaacgacca aaacgctgag
     1741 taaagcgggc aacattgcct tctgatgcca ccgttctgag tttaccggta tagaacggat
     1801 gcgatttgga ggaaacgtca atcgtcacat acggatacgt cacaccatcc agttcaatct
     1861 cgcgatctgt tttgatggta gaacctattt taaagtactc atccacactc gtgtcatgga
     1921 acactactgt gcggtattca ggatggatat ttggtttcat ctctcttcac tcatgttata
     1981 acataacaat aagatacatt ctcagctaga tcatttcaac agaatccccg agcccaaggg
     2041 taatgtggca tcgcagcctc tcatcaaatg gaagacatgt gattgcacat ttatcataca
     2101 agacgtatga taacactgtg ttatttatcc tgctgccgag ggtatagata tgaataacga
     2161 atctgaaaaa gcaatgtggc gttgtaagcc tgtggataat ccatggacgt tggattcatt
     2221 actaaaaaag actatttctg ctgttttacc tgcatcagac aaagtttcac accaaaataa
     2281 aaacaaatta cgtaataatg aattcattaa aattgagcat ccatgctcaa agtctgaatc
     2341 cagaatcgat ggtgactaca tcattagtgg aggaacagaa taccgtttgt aattaagcag
     2401 gagaattttc tgtaaattct cctgtaactt ttttacttcc tattttatac ctcgattagc
     2461 aatacttcct gcactcgaag ctgcaccttt cgcttccgaa ataccattcg gtaatcctcg
     2521 actgattcca tcgccaatat gaacaccagc ccaggacaac gctcccagcc acactgccgg
     2581 caagacgaga aacatcgtcc ccatcaccag attcattatc agatc
//
LOCUS       DAZFFI010000257         2615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFFI010000257 DAZFFI010000000
VERSION     DAZFFI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.259"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..521
                     /locus_tag="ACHJ12_005075"
     CDS             <1..521
                     /locus_tag="ACHJ12_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992704.1"
                     /translation="GIHLVIKNCSICKKMVPFYSDDNCLLCNKKNEELSPTRDEINKI
                     REFDVTISQDNLNSVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSAL
                     AFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYTELRNALACALSTCYNRKYLI
                     KVAKGKYLPVDD"
     gene            603..950
                     /locus_tag="ACHJ12_005076"
     CDS             603..950
                     /locus_tag="ACHJ12_005076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992705.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            1123..1287
                     /locus_tag="ACHJ12_005077"
     CDS             1123..1287
                     /locus_tag="ACHJ12_005077"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992706.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1291..1479)
                     /locus_tag="ACHJ12_005078"
                     /pseudo
     CDS             complement(1291..1479)
                     /locus_tag="ACHJ12_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1540..2615
                     /locus_tag="ACHJ12_005079"
                     /pseudo
     CDS             1540..2615
                     /locus_tag="ACHJ12_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
BASE COUNT          768 a          553 c          618 g          676 t
ORIGIN      
        1 cagggataca tctggtaata aaaaattgta gcatatgtaa aaagatggtg cctttttatt
       61 ctgatgataa ttgtttgctt tgtaataaga agaatgaaga gttgtcacca accagagatg
      121 aaattaataa aattcgcgag tttgatgtaa caatatccca agataatctt aattcagtta
      181 ctcaacgatt gttacccaca ccattagctg cggtaagagc ccgaagagtt gcatttgaaa
      241 atccatatag aaataaagta ctaagtgaat cattacctgc tggaaacctt ctttacagaa
      301 gtgcattagc ttttatacag gccttgattg cattagtccc atcaaagaaa atactggact
      361 tggataagat aacagatgct ctgcgtaggc ggtatgttca tcttgagcaa ttagaatata
      421 ccgaattaag aaatgcttta gcttgtgcat tgtcaacttg ttataataga aaatatctca
      481 ttaaagttgc aaaaggaaaa tatttacctg ttgacgacta ggtgcgtatt accgtgaagg
      541 agatcggtga gtaaccccct gtgtcagcat agttgtcgcc tcaatttttt aaggagataa
      601 caatgccagg acaaccaata ccaagtgagc tcaaagagaa aggactgata tggcttttac
      661 caccatactg ctggtcacat cgtcagatag cccgtaagct aaagatcagt gcctcagttg
      721 tatcaaaatg gagatatgag ttagtggagc aagggctttt acctgaaaat gaaaagatta
      781 cctccgttga tgttgaagac tggacaccgg agcgtcgatt ctcagttgtt cttgagacgg
      841 ctacgctgtc agaaatggag cttgctgatt attaccgacg catgggattg tttgttgaac
      901 aggttaaaga atggcgggct atctcaattc aggcgcatga aaaaaattga cggaaaatat
      961 tcgaacagac agggcattgc gtgacgcaaa aaataagtta cgagaactgt agaaagagct
     1021 taagcgtaaa gataaagctc tggcggaagc tgcggccctg cttatattga gggaaaagtt
     1081 caatgccctc tgggacaaca gcgaggaaga ctgacccctc tcctggagcg gcgtaaaatt
     1141 attgcccttg tccgcgaggc cgtctccaac ggcgcccgga aaagtcaggc ttgtaggtgt
     1201 atcggtattt caattcggac acttcagcgc tggactgatg gcaacgttat taccacggat
     1261 gtaagcgtca acggagcacc gtattgacgc ttatttattg gtgagtacta cgttccatgg
     1321 caggagttca tcaactcggt tggaaggcca ttccggcagt acgctcagga taaggcgcag
     1381 atacgcttcc ggatcgatac cgttcagacg gcaggtgccg atcagcccgt acaacagtgc
     1441 tccacgctcg ccgccgtgat cgctgccaaa gaacataaag atggtatgac ctttatatac
     1501 tcactatagc attggtggga tattccagag gaggtcatta tgcattcgga aaatattgca
     1561 gcttacatcg ggctggatgt acataaagaa acgttggcgg ttgctattgc tgcacctgaa
     1621 cggcttggcg aagttcgtta ttatggtacg attaacaacg aagcccaggc tgtacgtcga
     1681 ttatttcaga aattgcaggg actttacgga aacattcttt cctgctatga ggctggcccc
     1741 tgtggttttg ggctatatca tcaactcaca gcgatgaata taaaatgcca ggttattgcc
     1801 ccttcccgga taccaaaatc accgacagac agaatcaaaa atgatcatcg ggacgcaatt
     1861 tctcttgcca ggttactgcg ggctggtgaa cttacccctg tctggatccc tgacctgact
     1921 catgaagcta tgcgggatct aatcagagcc cgtgcagcag ctaaacgaga cagcagagtt
     1981 gcccgacagc gaattctgag catgctattg cgaacagaca aacgttatgc agggaagcac
     2041 tggaccggca aacatcgaac ctggttagcc aatcaatcat ttagtcagcc atctcaacag
     2101 attgcttttc aacactattg tcagagtctg gagcagattg aagatcgcat actttaactc
     2161 gaccaagaaa tatcccgcct gttacctgag tggtctctgt gcaatctggt ctgccagtta
     2221 caggccctta aaggtgttgg tcagctaacc gcaataaccc tggttgctga actgggtgat
     2281 ttttcacgat tttccagtcc caaacaactc atggcttttc tgggactggt tccaggggaa
     2341 tattccagtg gaaacagcat tcgtcccagg ggaataacaa aagttggaaa cagcgaactg
     2401 agacgtctgc tttacgaagc cgcctggtct tatcgtacac ctgcaaaagt tggtgcatgg
     2461 cttatatatt accgaccgga ttctgtaaca caatattcca aagatattgc atggaaagct
     2521 caacaacgat tgtgttctcg ttaccgaagt ctgacagcaa aagggaaaaa atcacaagta
     2581 gccattacgg cggtggctcg tgagttgact ggatt
//
LOCUS       DAZFFI010000258         2610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFFI010000258 DAZFFI010000000
VERSION     DAZFFI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.260"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..450
                     /locus_tag="ACHJ12_005080"
     CDS             <1..450
                     /locus_tag="ACHJ12_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4992707.1"
                     /translation="ECLIRQLITDEPSPVYFRDAVAFSSLALFEEHPDFHALLTGCIR
                     SCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQYAV
                     QLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            567..992
                     /locus_tag="ACHJ12_005081"
     CDS             567..992
                     /locus_tag="ACHJ12_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992708.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSGAGVELLPVEITPDEQKEPVAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            989..1339
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005082"
     CDS             989..1339
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4992709.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            1370..>2610
                     /locus_tag="ACHJ12_005083"
     CDS             1370..>2610
                     /locus_tag="ACHJ12_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4992710.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     AGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTS"
BASE COUNT          645 a          637 c          721 g          607 t
ORIGIN      
        1 gaatgtctga tcagacaact tattacagat gaaccatcgc cggtgtactt cagggatgcg
       61 gtggcttttt ccagtctggc gctgtttgag gaacatccgg atttccatgc cctgctcacg
      121 gggtgtatcc ggagctgcac tgtccgggtg aagaatatta tcagtgctga tttgtcccgt
      181 ccatggatgc tgggtgaagt ggccagtctg ttatatataa gtgagagttt actgaagaaa
      241 aagcttcagg aagagctgac gaccttcagt gagcttcttc tgaaagagag aatgcagtat
      301 gccgttcagc ttttgtctgc gggagaaaac cgtatttccc gggtggcaga gttgtgtggc
      361 tatgccagtg cttcttattt tatttctgtg ttcaggcgtt attatggtat taccccgaat
      421 cagtggcttc ctgtgatgca accggattaa tttgattcag atgcagagtg tgcgttattc
      481 gtaagcgtaa actgaccgcc gtatgtagcc attaagccag tattggtaac gtaggtgccc
      541 accttttcaa ctagtggaca cctgttatgg aacagaaaat attatcctca gaaccccgca
      601 gatcattttc aaatgagttt aaacttcaaa tggttaaact ggcttcacaa ccaggagcct
      661 ctgttgcccg tattgcccgg gaacacgata tcaatgataa cctcctgttc aaatggctca
      721 ggctctggca gaacgaaggg cgtatatcgc ggcgtcttcc ggtaacaacc tcttctggcg
      781 ctggcgttga attattacct gttgagataa cgcctgatga gcagaaagag cctgtggcgg
      841 ctcttactcc gcttttatcc actccttcac aaagcacagt cagtgccagt tcctgtaaag
      901 ttgagttccg ccacggtaac atgacgctgg aaaatccttc accagagctg ctcacggtgt
      961 tgatccgtga actgaccggg aggggacgat gatctcgctc ccatcaggca ctcgaatctg
     1021 gctggttgcc ggcgttactg atatgcgtaa atccttcaac ggtctgggag aacaggtaca
     1081 acatgtgctg gatgagaacc ccttctccgg tcacctgttt atctttcgcg gccgacgggg
     1141 tgacacgatt aaaattctgt gggctgatgc tgatggtctg tgcctgttca ccaaacgtct
     1201 ggaggaaggc cagtttatct ggcctgcggt gcgtgacggt aaggtatcca ttacccgctc
     1261 gcagctggca atgctcctcg ataaactgga ctggcgtcag cccaaaacat cccgccttaa
     1321 tgcactgaca atgttgtaaa aatgtcatgg ccggattata aaaacggtca tgagtcggaa
     1381 atacctcatt cgtatcactg agctggaaag gctgctctct gagcaggctg aagccctccg
     1441 tcagaaagat cagcaactga gtctggttga agagacggag gccttcctgc gctctgcact
     1501 ggcacgtgcc gaggaaaaga tcgaagagga ggagcgggaa atagaacatc tgagggccca
     1561 gatagaaaaa ctgcgccgga tgttgttcgg tacccgttct gaaaaactgc agcgtgaggt
     1621 tgaacaggct gaggcccaac tgaaacaacg cgaacaggaa agcgatcgtt acagtgggcg
     1681 tgaggatgac ccgcaggttc cccgccagtt gcgacagtca cgtcatcgtc gtccgttacc
     1741 ggcacatctt ccccgtgaaa tacaccgtct ggagcctgaa gaaagttgtt gcccggagtg
     1801 tggcagtgag ctggattatc tgggggaagt cagtgctgag cagctggaac tggtgagtag
     1861 cgccctgaaa gtgatccgca ctgttcgggt aaaaaaagcc tgtacaaaat gtgactgtat
     1921 agttgaagcg ccagcgccgt cccgcccgat agagcgcggc atcgcgggct ccggattact
     1981 tgcccgcgtg ttaacgggaa aatactgcga acacctgcca ctgtatcgtc agagtgaaat
     2041 ctttgcccgc cagggcatcg aactgagccg ggcattactc tccaactggg ttgacgcatg
     2101 ttgtcagtta atgactctgc tgaatgatac cctgtaccgt tacgtgatga acacccgcaa
     2161 ggttcacact gacgacacac cagtaaaagt gctggcagcg ggcagaaaaa aggcaaaaac
     2221 aggacgcatc tggacgtatg tccgggatga ccggaatgcg ggctcatcag agccaccggc
     2281 ggtctggttc gcctactcac cagacaggca gggaaaacat ccggtacaac accttcgtcc
     2341 cttccggggt atcctgcagg cggatgcgtt cagcggttac gatcggctgt tcagtgcaga
     2401 acgtgaaggt ggtgcactga cagaagttgc gtgctgggcc catgcccggc gaaaaatcca
     2461 cgatgtatac atcagcagca aaagtgcgac ggcagaagaa gccctgaagc gaatcagtga
     2521 actgtacgcc atcgaggatg aaatacgggg attaccggag tcagagcgtc ttgccgtcag
     2581 gcagcagcga agcaaagtgt tactgacgtc
//
LOCUS       DAZFFI010000259         2598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFFI010000259 DAZFFI010000000
VERSION     DAZFFI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.261"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..607
                     /locus_tag="ACHJ12_005084"
     CDS             5..607
                     /locus_tag="ACHJ12_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4992711.1"
                     /translation="MNVYLNSLKKEDERIDAINKIKIELHRSSPFYAEPVDCVIWIKN
                     NLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGFHRQMLGKST
                     TKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIVRDLSRMGWD
                     DLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(789..2534)
                     /locus_tag="ACHJ12_005085"
     CDS             complement(789..2534)
                     /locus_tag="ACHJ12_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO4992712.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          792 a          637 c          524 g          645 t
ORIGIN      
        1 tgaaataaat gtatacctta attctcttaa gaaagaagat gaacgcattg atgcaattaa
       61 taaaataaag attgagttac ataggagtag tcctttttac gcagaacctg ttgattgtgt
      121 tatatggata aaaaacaatc taattgaagc aaacgactat aatcctaacg tcatggctcc
      181 ctccgaaaaa aggttattaa cacactccct gttggttgat ggattcactc aacctgttgt
      241 caccttagct caaaagggga aatacaccgt tattgacgga tttcacagac aaatgcttgg
      301 gaaaagcaca acaaaactag agaaaaaact aaaaggatat ttacctgtca catgcattaa
      361 gcatgataat aactttaaag cttactctat cgcctcgacc atcaggcata acagggccag
      421 aggaaagcac cagatagacg cgctctccga tatcgtccga gatctttccc gaatgggatg
      481 ggatgatctc cgaataggta gtgaactggg catggataca gatgaggtac tgagattaaa
      541 acaaataagt gggttaacag aactatttga ggaggaaaac tttagtccgg catggacaat
      601 aaagtaatca tttagggggt gactttatcc agtaattacc gactgatata tgatcacccc
      661 atgtaatgga cagctattga cagatcaaaa acatggggga tattaattca actctgaata
      721 ctcgctaaat acccatctta gattatcaaa aaacgaatga ttacatatca gcaataataa
      781 aacacaactt atacaaaatc ctcaagatga cgactgatac tgttttcacg ataacaggtc
      841 accagtaatt cccgtgtagc ccgggtgaac gcaacataaa gcgccggaat aacttcagac
      901 tcatccgcag cacctgagtg aacgaaagaa gcattgataa ccgcaacata cggaaactcc
      961 agtcccttac tgctctggta ggtcagtaaa tgcacaacat ccttgcgtcg cgaatatttc
     1021 ttcttacctt cactgtcaaa gcaggtggca taaggtatcc cgcgttgtgt catgacctct
     1081 ttcagatgtt tcactgaaaa ctctgcagga cacaaaacag ccatatcact ccagtgaccg
     1141 cacagtgtat agcgtttctc cagccagccc acaacctgca ctgcctcatc actctcagaa
     1201 gcacaacgca gaatttccgg ctctgtgccc tcttcaccgc ccgcctgtgg ctgaacaaac
     1261 ggtatttcac ggttctgatg cttctcaaaa tactcacggg agaatgcata agcaaagtgc
     1321 agaatgcgtt tcgtattacg gtaatttacc ggcaggacgg aagtacgtcc ctgcgcctga
     1381 atccccacac tggcaagcga aaagttcaga gcccgttccc gtctgtaaat ggactgtgcg
     1441 tcatcgtaca tcaggagcag tgaacgggac gcgttatcaa acaggcgggc tatcagagcc
     1501 agccagcgac tctcaaaatc atgcccctca tccacaagta cggcatcgta tccagtatca
     1561 gtaattgttc cgctgttcac ggcatcttcc agcgcactga agcatttctc cgggtaatcc
     1621 tttcctccag cagtcacctg aattccatgg cgctttatca ttgaggcgca ccaactatga
     1681 aaatggaata ccgtaacccg tgacttcagt cccctggact caatacattc ccgcaggtag
     1741 ctggccagag taatgttgta acatagcacc agaatcttac caggggtagt ttcagccaag
     1801 taaagacaac ggaacaaaag aatcattgtt tttccagagc cggctacccc atgaataaca
     1861 cgatgcccat caccaatatt tctggccaga atctcctgct gaatatccat tattttgatt
     1921 tgtgagtgct gtctgacggt catttcagga aacagatgag cacgcagaat atctctaacc
     1981 tgtggtgtca cccgggttct gaatcccatc gtaaacatcc cggcaatttt ttggcggaat
     2041 atatcaggta aaacagactg agtcatatca tcctgacaga ttgtctgcgc tgaagggaaa
     2101 atcctttcca cagcattttc atcattccca gccagagcct tcagttgctg gcgggtaata
     2161 cgggtaaaca caacaccata cgcccaggcc agattcagac gccccttata ctgaccatca
     2221 ttctgtcgca aacgtgagtc tgcaggtaaa gcatttacgg tatcacaggc ataacgacga
     2281 acctgtagca gcggatttat ttctgattta agaataccgt tagtttcaag aatgacctgt
     2341 tcgtgattta cctgatgcaa cgtgtgaacg gtccagtcct tgacttcaag aaataccaga
     2401 ccattctccg ggtcaataat gacaaaatca ggatgtcggt ttttccgtcc caccggaata
     2461 tcgtaccaga ccagacactc attactcata aatgcctcaa gacgggatgc aacccgcttt
     2521 tccccggcag tcatccgctc ctcggtaaca cctgatatat ttatcagttt agccataaaa
     2581 cagcccctga cagatgat
//
LOCUS       DAZFFI010000260         2591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFFI010000260 DAZFFI010000000
VERSION     DAZFFI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.262"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..594
                     /locus_tag="ACHJ12_005086"
     CDS             <1..594
                     /locus_tag="ACHJ12_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4992713.1"
                     /translation="YALMQGQTFDKSAYPKLATAYPSGVIPDMRGWTIKGKPASGRAV
                     LSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHT
                     HSVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAH
                     THSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            594..1169
                     /locus_tag="ACHJ12_005087"
     CDS             594..1169
                     /locus_tag="ACHJ12_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4992714.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(1267..1857)
                     /locus_tag="ACHJ12_005088"
     CDS             complement(1267..1857)
                     /locus_tag="ACHJ12_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4992715.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
     gene            complement(2176..2409)
                     /gene="ydfK"
                     /locus_tag="ACHJ12_005089"
     CDS             complement(2176..2409)
                     /gene="ydfK"
                     /locus_tag="ACHJ12_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO4992716.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVMEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNGKM"
     gene            complement(2478..2591)
                     /locus_tag="ACHJ12_005090"
     CDS             complement(2478..2591)
                     /locus_tag="ACHJ12_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO4992717.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          702 a          534 c          591 g          764 t
ORIGIN      
        1 tatgccctga tgcaggggca gacttttgac aaatctgctt acccgaaact tgcaaccgct
       61 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa acctgccagt
      121 ggtcgggccg tattgtctca ggaacaggac ggcattaaat cgcacaccca cagcgccagc
      181 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
      241 acgaataaca ccggggcgca tacgcacagt gtgagcggta cagccgcaag tgccggaaac
      301 catactcata gtgtcacagg cgcatcagca gtcagccagt ggtcacaaaa tgggtcagta
      361 cataaggtag tgtctgcggc cagtgtgaat acaagtgctg caggagcgca cactcatagt
      421 gtcagcggca cagctgcatc tgcaggtgct cacgcacata ctgtcggtat tggtgctcat
      481 acgcactctg ttgcgattgg ctcacatgga cacaccatca ccgttaacgc tgctggtaac
      541 gcggaaaaca ccgtcaaaaa catcgcattt aactacattg tgaggcttgc ataatggcat
      601 tcagaatgag tgaacaacca cggaccataa aaatttataa tctgctggcc ggaactaatg
      661 aatttattgg tgaaggtgac gcatatattc cgcctcatac aggtctgcca gcaaacagta
      721 cctatattgc accgccagat attccggctg gcttcgtggc tgttttcaac agtgatgagg
      781 catcgtggca tctcgttgaa gatcatcggg gtaaaacggt ttatgacgtg gcttccggcg
      841 acgcgttatt tatttctgaa ctcggctcat taccggaaaa tgtcacctgg ttatccccgg
      901 aaggggagtt tcagaagtgg aacggcacag cctgggtgaa ggatacggaa gcagaaaaac
      961 tgttccggat ccgggaggcg gaagaaacaa aaaacagcct gatgcaggta gccagtgagc
     1021 atatagcgcc gcttcaggat gctgtggatc tggagatcgc aacggagaaa gaaaccttgt
     1081 tgctggaagc ctggaaaaag tatcgggtgt tgctgaaccg tgttgataca tcaactgcac
     1141 cggatattga atggccagcc ctaccgtagg gtaagatata taaattctat aattagaagt
     1201 atctttccat ttaaggctag gaaggggggc ttggaaaacg taaggaatct cacaccgaga
     1261 ttatttttat atatcaggtg tctgattttt tgctttagct cttaaaatgg tttgccgcga
     1321 ggttttgaat tcttgggcaa tgtcacttat atttatacct gacttaattt gctctagtac
     1381 cctctgtatt tgttcatcat ttaacacagg tgggcgacca aatcgctttc ctgcgccccg
     1441 tgctcttaat atccctgaat gagtacgctc aagtaaaagg tctcgctcaa attcagcgac
     1501 tgctgaaatt acttgcatca tcatttttcc aggtggactg gtcaggtcaa cgccccccaa
     1561 tgctaagcaa tgcactttga tacctgcttt ggtcagttgt tccaccgttt tactgatatc
     1621 cattggatta caaccgaggc gatccagttt tgtgacaatc aatgcatcac ctcttttcag
     1681 tcgatcaagc aactggttaa aaccaggacg ctcactggtt gctgctgagc cgctaatttg
     1741 ttcttcaatt atttgctgag atttgatgtt aaaacctgca ctttcgattt cccgacgttg
     1801 atttttggtt gtttgttcca gagttgatac ccgacagtaa gcataaattc gagacatagt
     1861 gagatcttct atacgaaatt ggtgtacata tcataatgca tctcagaaaa taattttgat
     1921 tatttttgta catatttgta tgtacacgtt cgaaaataaa cgaatgcgta tgcaaccccg
     1981 taattttggt gagatccaaa atcgattttg tgaaaaatgg ccttaactcg gtttgttttt
     2041 cgagttccgg gcggactcaa ggaagaagaa tagtgttgcg tgttatttta accagatttc
     2101 aagttgtttg gtcgtggaaa agtggagcaa aatgttgtta aagtggaaaa atgataaaaa
     2161 agtaagttta ttatattaca ttttaccatt taaatttttg ttgtctttaa gaactgatat
     2221 cgctgtttgt aataattctt tgttatccag ccatgacttt ttctttatgt ttccttcaat
     2281 gtaatcaagc aatgttctgg tattgatagg tcttccctgt tttgctactt ccattacagc
     2341 atcccctagg ataattctta cttcaggaag ctgcgcaggg aaccacttta gggtgtcttt
     2401 tgatttcatg aagatattcc ttaaaatatt attgattttc attgcgatat tgtatgtctg
     2461 attcaggata tgttgactta tacatcggtt ttgtctgggt tattggatat gccaatccct
     2521 aattttatta gagcatgact aaaaatgctg aatatgataa ggagcgaagt gattatcagt
     2581 atgctgttca t
//
LOCUS       DAZFFI010000261         2589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFFI010000261 DAZFFI010000000
VERSION     DAZFFI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.263"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..174
                     /locus_tag="ACHJ12_005091"
     CDS             <1..174
                     /locus_tag="ACHJ12_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO4992718.1"
                     /translation="AVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVG
                     GSQVFSFAEHGLL"
     gene            237..458
                     /locus_tag="ACHJ12_005092"
     CDS             237..458
                     /locus_tag="ACHJ12_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4992719.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            621..995
                     /locus_tag="ACHJ12_005093"
     CDS             621..995
                     /locus_tag="ACHJ12_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4992720.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYL
                     ADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHSVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            1042..1419
                     /locus_tag="ACHJ12_005094"
     CDS             1042..1419
                     /locus_tag="ACHJ12_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4992721.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            1416..1904
                     /locus_tag="ACHJ12_005095"
     CDS             1416..1904
                     /locus_tag="ACHJ12_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4992722.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            1916..2113
                     /locus_tag="ACHJ12_005096"
     CDS             1916..2113
                     /locus_tag="ACHJ12_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4992723.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2198..>2589
                     /locus_tag="ACHJ12_005097"
     CDS             2198..>2589
                     /locus_tag="ACHJ12_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4992724.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAA"
BASE COUNT          646 a          737 c          622 g          584 t
ORIGIN      
        1 gccgtggtgc tggcacacaa tcacccgtcc ggtgaagtca cccccagtaa ggcagaccgc
       61 cttatcacgg aacgtctggt acaggcactg ggcctggtgg atatccgggt gccggaccat
      121 ctgatagtcg gtggcagcca ggttttctcc tttgccgaac atggtctgct ttaacccgtc
      181 acaaccacat cacacctgtt ttcactttta tcttctgtct tcagaggtat cccattatga
      241 aaattatcac ccgtggtgaa gccatgcgca ttcaccggca gcatcctgca tcccgtcttt
      301 ttccgttctg caccggcaaa taccgctggc aaggcagcac tgaagcgtat accggtcgtg
      361 aagtacagga tattcccggt gtgctggccg tgtttgccga acgccgtaag gacagttttg
      421 gcccgtatgt ccggctgatg agcgtcaccc tgaactgatg ttccgtcacc cgggaaacga
      481 gtacgaccag atgacctcag acgccgctgt ggatgaggcc attctcatca atgcatacat
      541 cttcaccgaa accggtcagt gccccggctg acctgtcatc gttaaaccat tcttaaccac
      601 ttttcagaga ggattttatc gtgtcagaca aactctccgg gataactcat cccgacgaca
      661 atcacgaccg cccctggtgg gggcttccct gcacagtgaa accctgtttt ggcgcccgtc
      721 tggtgcagga gggtaaccgg ttgcattacc ttgccgaccg cgccggtatc agcggccgct
      781 tcagcgacgc agatgcatac catctggacc aggcctttcc gctgctgatg aaacaactgg
      841 aactcatgct caccagcggt gaactgaatc cccgacatca gcattccgtc acgctgtatg
      901 caaaagggct gacctgcgaa gccgacaccc tcggctcctg tggttacgtt tatctgactg
      961 tttatccgac accggcaacc gcaaccacct cataaccccg aagctctcac ctttagcact
     1021 accagttaca gagaacttaa gatgaaaaca ttatccgaca cacatgtacg ggaggcatct
     1081 cgctgcccgt ctcccgtcac catctggcag acactgctca tccgactgct ggaccagcat
     1141 tatggcctca cactgaatga cacaccgttt gccgatgaac gtgtgattga gcagcatatt
     1201 gaggcaggca tttcactgtg tgatgcggtg aactttctcg tggaaaaata cgcgctggta
     1261 cgaactgacc agccaggatt cagcgcctgt acccgctctc agttaataaa cagcatcgat
     1321 atcctccggg ctcgccgggc aacaggcctg atgacccgcg acaattacag aatggtaaat
     1381 aacattaccc agggtaagca tccggaggcg caacaatgaa actggccctg acactggaag
     1441 ccgacagcat taacgtgcag gcactgaaca tggggcgcat tgtcgttgac gtcgatggca
     1501 ttgagctcgc tgaactgatt aacaaagtcg ctgaaaacgg ttattcactc catgtggtgg
     1561 acgactccga ccaacagtca acctgcacac caccaccgtt aacaaccctt accggcatac
     1621 gctgcagcac cgcacatatc acggaaacag acaacgcctg gttgtactcg ctgtcacacc
     1681 agaccagtga cttcggcgaa tcagaatgga ttcattttac aggtagcgga tatctgttac
     1741 gtaccgatgc gtggtcatac ccggttctgc ggcttaaacg cctggggctg tcaaaaatgt
     1801 tccgccgtct ggttgtcaca cttatccggc gttatggcgt cagtctcatt catctggatg
     1861 ccagcgctga atgcctgccg ggtttaccca ctttcaactg gtaaccagaa acaacatgaa
     1921 atcattaacc actgaaacag cgctggatat cctcattgcg tggctgcagg acaatatcga
     1981 ctgcgaatcg ggaattatct ttgacaacga tgaggataaa acggattcgg cagcactgtt
     2041 gccctgtatc gaacaggccc gggaggatgt ccgtactctt cgtcatctgc agcttctgca
     2101 ccagaaccgg taaatatcac tcaccatact tcattccact gacgccagcc tgaacagggc
     2161 tggcgttttc atttatctgc aaaaaggaaa aacgattatg tcaggtatca caatctcccg
     2221 tcctgaagtg gtcaacgggc atacggacgt tatctgttcc acctcaatcg gacacattct
     2281 ggctgtacga aagaccacac tgatgcaaat cgacacactt atccggcaac tgacagaaat
     2341 ctcagcaatg acagaaagta ttggaggtaa aaccgccccg gactgggcca tgaaacagaa
     2401 ttttcgctgc ggttgctggc tgatggagaa acccgaaaca gcaatgaaag ccattacccg
     2461 gaatcttgac cgcgaaatct ggcgtgacct gatgcaacgt tccgggatgc tgtcattaat
     2521 ggatgcacag gcacgtgata catggtaccg gtcactggaa tacgataatt ttccggaaat
     2581 cagtgcggc
//
LOCUS       DAZFFI010000262         2553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFFI010000262 DAZFFI010000000
VERSION     DAZFFI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.264"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1073)
                     /locus_tag="ACHJ12_005098"
     CDS             complement(<1..1073)
                     /locus_tag="ACHJ12_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4992725.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISQADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRA"
     gene            complement(1121..1330)
                     /locus_tag="ACHJ12_005099"
     CDS             complement(1121..1330)
                     /locus_tag="ACHJ12_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4992726.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEHALSFAAQS"
     gene            complement(1426..1800)
                     /locus_tag="ACHJ12_005100"
     CDS             complement(1426..1800)
                     /locus_tag="ACHJ12_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4992727.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESVRKK"
     gene            complement(1815..2531)
                     /locus_tag="ACHJ12_005101"
     CDS             complement(1815..2531)
                     /locus_tag="ACHJ12_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4992728.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGVLLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
BASE COUNT          542 a          848 c          608 g          555 t
ORIGIN      
        1 tgagcccggt acacggctgc ccttgcccgc gccgtggcaa tctgcgttcc ccttaactgg
       61 gcctcagcca gtgccacttc attacgtgca gcggtcacaa gtcctgccgt ggcagacatc
      121 gctccggaag ccagattacc aaagtaccgg gccaccccga cagcaaccag cacacccgca
      181 gctgttgcca cggtatcaat atttccggcc acaccgttca gtgcgccgga gagcgttttc
      241 gtcgctccgc tggcctcatt cgcgccgccc acccaggcca taaaggcgtt ttccaccttc
      301 gtgatcccgt cagacaccgt ttccggcatg gctgcatatt catcacgcaa tatccccagc
      361 tggctgatta acgcgggaac gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc
      421 ttcagatcct tacgggccac gcccatgcct gcagccagtg cgcgaatgat ccggtcccca
      481 ctttcattga ccgaattaaa ttcctcgccg cgcaacaccc cctgtgccag cgcctggctg
      541 aactgggtga tcaccgaacc cgcctctgcc gcactggcac cggaaatttt cagccctgtc
      601 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcatcgag
      661 gcagccgaac gggcaaacag ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg
      721 atgtccatca gcactttctg tgatgacgaa aattcatccg atgactgcga cgcctgtttc
      781 agacgggcat tcacggagct ccattcatcc gcctgcgaaa tcaggtgtcc ggtggcaaag
      841 gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
      901 ttcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg acgtccgcca
      961 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
     1021 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
     1081 tctccgggcg taaaaaaacc gcctcagcgg ttctcatcat tcatgactgt gctgcaaagc
     1141 tcagcgcgtg ttccagcgcc gcaaacggat ccacctccgg cttatcctca tcctcgcccc
     1201 agcagagcat ggcgtccttc agtgcaacat tcatcccctg tgccccgaaa accgctttca
     1261 cgatctgtgc attacggata tccccgcgct catcacccag cggggatatc ctgtcgaact
     1321 ccatccacat catcgcctcg ctcgcactca ggctgtgccg cagttcggat aaggtgcgcc
     1381 ccagacggag cgcaagtcgc atcagaaagc gaatttccgg gcgggctact tttttctgac
     1441 cgactctgca tcagcgatca gttccagtgc ctgacgcagc aagcgggcat gtaccggacc
     1501 atagacggcc agcacctgct cacggtcgtc cggagtgaac acccgttgca gatccgtatc
     1561 acacaggaca tcgcagaaca gcgtcacatc cgcttccagg ttacggcggg ttttcgccac
     1621 caccgacagg gtatcatcat cctctccatc accattgagc acgtcctgcc acagatacca
     1681 ggcctctgcc gaaggctccc gcagcaccac gctgacatta ccccattccg gcaccttcac
     1741 cgttttatga cgaaaccctg acagtctggc cagcgccagc gttttcagat cctttttcat
     1801 gatgacccat ccccttatcc ggcggctgcg ctcactgtca cggtgcattc aacagacgtc
     1861 acactctgtg ctttctctgc cgaatcagtc accacgcagg tatatttccc cgcatcggcg
     1921 gactgcgcac ctggcttact gaaggtgtct gtcgtctgcc cgtcaaccgg ctgaccatcc
     1981 ttcttccagg cgtatttata cggcggcgtt cccccgttgg cactgactga cattgtcagc
     2041 agcacaccgg tattcacggt aagtgtctta tccagatttt tcacaaacgc cagcggtacc
     2101 acaaaggaca ccggtttgcc tttcagacgc agtgagaacg ttgcagccac cacgccgttg
     2161 gtaccggatg accaggtgtg ctgacgcact tccgccagga atttaaagcc cttaccggac
     2221 ggaaactgca ccttaaacgc atacaacgcg tcattgtcat aggcatcacg cagggcgttc
     2281 tgggcctgat tcagataaaa attacccgac atggaaatct cggacgacgc ccccagaccg
     2341 ttgatgttct cctgctctgt ggagcagagc gtggtcacat caatatcctg tttctgaccg
     2401 gcggtgaact ggacttcctt gatggtgcag tccaggcgca gatattccgc cttatccata
     2461 gtttcagcag tcgccggggc agatgaaatc atcacctgcg tcagctgtga gcgttcatac
     2521 aaagcagaca ttctgcctcc tgataataaa aaa
//
LOCUS       DAZFFI010000263         2530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFFI010000263 DAZFFI010000000
VERSION     DAZFFI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.265"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            343..906
                     /locus_tag="ACHJ12_005102"
     CDS             343..906
                     /locus_tag="ACHJ12_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4992729.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            903..>2530
                     /locus_tag="ACHJ12_005103"
     CDS             903..>2530
                     /locus_tag="ACHJ12_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4992730.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEP"
BASE COUNT          570 a          618 c          818 g          524 t
ORIGIN      
        1 ctgccggagg tgtccggagg tagtggcaga gcggacgaca ttatcggact gtaacccgac
       61 cgggggggga tcatccggcg taaaaaacga tcgctccgga caccgcgccc cctctcatgc
      121 agagaaaaaa ttcccgtttc agggcagtta acatgttaac tggctgccca ggcatttttg
      181 cggtttttat ctttattatt cagtttgttg cgcggaaaaa atgttaacag gctttttcag
      241 caaatgttaa ccaggcagca gttaacattt gcggcatgag acgccgggaa aaatgggctg
      301 aaccataccc ggctgagtgc gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa
      361 cgaaaatttg cgctggcgct catgtccggg aaaaacaaaa cagcgtcagc cattgccgcc
      421 ggttattcgg cgaagacagc cagggttaaa ggctcgcagc tggcaaaaga tccggaggtg
      481 cttgcgttta tagcccgtaa gcagtgcgag acggtggagg tggatgaggt tcctgtttac
      541 cggcagaaaa aatcagagca ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca
      601 cagccggacg aaacaaatcc ggagatgcca ccgcccgtgg tgatatctcc tggtattgag
      661 tatatggagg acggtcttcc cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac
      721 attaataccg accccaggct ggcgctggat gcggcttata agctggcgca gttcacgcac
      781 cacaaaaaag gggatgccgg taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct
      841 aaccgttttg cggtgccacc accaccccga ctggtggtga ataatgataa tgagggcaac
      901 ggatgatacc tgtgtggagc acggcctgcc cggactgggc agagcgcctg aaaaaggggc
      961 tgtcgattat tccggctccg atttatccgg agcaggccgc acatgccctg gcgattttta
     1021 aacaactgcg gattgtggat gcaccgggca gcccgacgtt cggggagtcc tgcgcaccgt
     1081 gggtgtttga cctggtggcg gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc
     1141 atatcaagga agtttttatc cttatcccca agaaaaacag caagtccacg ctggctgccg
     1201 ggatcatgat gacggcgctg ttactgaact ggcggcaggc ggcgggctac accattctgg
     1261 ccccgaccgt ggaggtggcg gctaacgcct ttaatcctgc gcgggatatg gtacgacggg
     1321 acgatgatct ggatgacctc tgtcaggtgc agacacatat ccggaccatc acccatcggg
     1381 tgacggacac cacccttaag gtggtggctg ccgatccgaa tacggtatcc ggtatcaagt
     1441 ccgtggggac gctgattgat gaactgtggt tatttggcaa gcagtacaaa gcggaggaca
     1501 tgttacgtga agccataggc ggccttgcct cccgcccgga agggtttgtg gtgtatacga
     1561 ccacccagtc gaatgaaccg cccgccgggg tgttcagaca gaaactgcag tacgcccggg
     1621 atgtccgtga cggcaaaatt catgatccgc actttctgcc ggtgatattt gaacaccctc
     1681 ctgaaatggt ggaaagcggg gctcacctgc tgatggaaaa cctcgccatg gtcaatccga
     1741 atctcggtta ttcggtggat gaggcttttc tgtaccggga gtaccgtaaa gcccgggagg
     1801 ctggtgagga agcatttcgt ggcttcatgt caaaacatgc caatgtggaa attggtcttg
     1861 ccctgcgttc tgaccgctgg gcgggtgcgg atttctggga gcagcagggc aggcgcgtca
     1921 gcctggacga tatcctgcag cgcgctgatg tggtgacggt ggggattgac ggcgggggcc
     1981 tggatgatct gctgggaatg tacgtgattg gccgtgacag ggaaacccgc gaatggctgg
     2041 gctggggcca tgcctgggcg catgaaaccg cggtggtcag acggaagagt gaggcatccc
     2101 ggtttcagga ttttgtggcc tgtggagata tgacgattgt ccgtcgggtc ggggatgaca
     2161 cggcggaagt ggcggagtat gtgcgtcgta ttcatgaggc tgagttactg gagcatatcg
     2221 gtattgaccc gtcaggtgtg gggcagattc tggattcact ggtggaagcc gggatccccg
     2281 acggaattgt tgtggggata agccagggct ggaagctggg cggggccata aaaaccaccg
     2341 agcgcaaact ggctgaggga gtactggtgc atggtggtca gccactgatg gcctggtgcg
     2401 ttggcaatgc ccgggtggag ccgaaaggta acgccatcct tattaccaaa caggccagcg
     2461 gacgggggaa aattgacccg ctgatggcgc tcttcaatgc ggtatccctg atgtccctga
     2521 atccggagcc
//
LOCUS       DAZFFI010000264         2511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFFI010000264 DAZFFI010000000
VERSION     DAZFFI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.266"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..174
                     /locus_tag="ACHJ12_005104"
     CDS             <1..174
                     /locus_tag="ACHJ12_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO4992731.1"
                     /translation="AVVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVG
                     GSQVFSFAEHGLL"
     gene            243..464
                     /locus_tag="ACHJ12_005105"
     CDS             243..464
                     /locus_tag="ACHJ12_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4992732.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            540..917
                     /gene="cbeA"
                     /locus_tag="ACHJ12_005106"
     CDS             540..917
                     /gene="cbeA"
                     /locus_tag="ACHJ12_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4992733.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            964..1341
                     /locus_tag="ACHJ12_005107"
     CDS             964..1341
                     /locus_tag="ACHJ12_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4992734.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLEQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1338..1826
                     /locus_tag="ACHJ12_005108"
     CDS             1338..1826
                     /locus_tag="ACHJ12_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4992735.1"
                     /translation="MKLTLTPEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1838..2035
                     /locus_tag="ACHJ12_005109"
     CDS             1838..2035
                     /locus_tag="ACHJ12_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4992736.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            2120..>2511
                     /locus_tag="ACHJ12_005110"
     CDS             2120..>2511
                     /locus_tag="ACHJ12_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4992737.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKA"
BASE COUNT          609 a          710 c          622 g          570 t
ORIGIN      
        1 gccgtggtgc tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg
       61 cttatcaccg aacgtctggt acaggcgctg gccctggttg acatccgggt gccggaccat
      121 ctgatagtcg gtggcagcca ggttttctcc tttgcggaac acggtctgct ttaacctgtc
      181 accgtcacaa ccacatcata cctgttttcg cttttatctt ctgtcttcag aggtatcaca
      241 ctatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacaacat ccggcatccc
      301 gtctttttcc gttctgtacc ggcaaatacc gctggcacgg cagcactgaa gcgtataccg
      361 gtcgtgaagt gcagaatatt cccggtgtgc tggctgtatt tgcagaacgc cgtaaagaca
      421 gttttggccc gtatgtccgg ctgatgagcg tcaccctgaa ctgaatcagg gcattcagat
      481 gagcagaatt atcgccacca tcgaaccatt cttaactgat tttctgtgag gactttatcg
      541 tgtcagacac actccccgga acaacgcatc ccgacgacaa tcacgaccgc ccctggtggg
      601 ggctgccctg caccgtgacg ccctgtttcg gggcacgtct ggtgcaggag ggtaaccgcc
      661 tgcattacct tgccgaccgc gccggtatca gaggcctgtt cagcgacgcg gatgcgtacc
      721 acctggacca ggcctttccg ctgctgatga aacaactgga actcatgctc accggcggtg
      781 aactgaatcc ccgccatcag cataccgtca cactgtatgc gaaagggctg acctgcgaag
      841 ccgacaccct aggctcctgt ggttacgttt atctggctgt ttatccgaca ccggcagcgc
      901 ccgcaatcac cgtataaccc cggatatctc ccctttatca ccacctgtta cagagaactt
      961 aagatgaata cattacccga cactcacgta cgggaggcat cgggctgccc gtctcccgtc
     1021 accatctggc agacactgct cacccgactg ctggagcagc actatggtct cacgctgaat
     1081 gacacaccgt tcgctgatga acgtgtgatt gagcagcata ttgaggcagg gatttcactg
     1141 tgtgacgcgg tgaactttct cgttgaaaaa tacgcactgg tgcgtaccga ccagccggaa
     1201 ttcagtgcct gtacccgttc tcagttaata aacagcatcg atatcctccg ggctcgcagg
     1261 gcgaccggcc tgatgacccg cgacaattac agaacggtaa ataacattac ccaggggaag
     1321 catccggagg cgaaacaatg aaactgactc tgacgccgga agctgacagc gttaacgtgc
     1381 aggcgctgaa catgggacga attgtcgttg acgtcgatgg tgttaatctc gctgaactga
     1441 ttaacgtggt ctgcgacaat ggccactccc ttcgtgttgt tgatgaatct gaccgggtct
     1501 caacagactg cacgccacca tttgctgccc tgaccggcat acgctgcagt accgcgcata
     1561 tcactgcaaa ggacaacgcc tggctgtact cgctgtcaca tcagacgaat gacaccggcg
     1621 aatcagaatg gattcatttc acaggtagcg gatatctgtt acgtaccgat gcgtggtcat
     1681 acccggttct gcggcttaaa cgcctggggc tgtcaaaaac gttccgccgt ctggttgtca
     1741 cacttacccg acgttatggc gtcagtctca ttcatctgga tgccagcgct gaatgcctgc
     1801 cgggtttacc cactttcgac tggtaaccag aaacaacatg aaatcattaa ccacggaaac
     1861 agcgctggat attctgattg cgtggctgca ggacaatatc gactgcgaat cgggaattat
     1921 ctttgacaac gatgaggata aaacggattc ggcagcactg ctgccctgca ttgaacaggc
     1981 cagggaagat atccgtacac tgcgccagtt gcagcttctg caccagaacc ggtgactctc
     2041 actcaccaga cttcattcca ctgacgccag cctgaacagg gctggcgttt tcatttatct
     2101 gcaaaaagga atatcgatta tgtctgatat cacagtctcc cgtccggaag ttgtcaacgg
     2161 gcatacggac gttatctgct caaccagtat cctccacatt ctggctgtac gaaagagtac
     2221 actgctgcaa atcgacacac ttatccggca actggctgaa atctcagcaa tgacagaaag
     2281 cattggcggt aaaaccgcac tggactgggc catgaaacag aattttcact gcggttgctg
     2341 gctgatggag aaacctgaaa ccgcaatgaa agccatcaca cgcaatctcg atcgcgaaat
     2401 ctggcgtgac ctgatgcaac gttcagggat gctttcctta atggatgcac aggcccgtga
     2461 tacatggtac cggtcactgg aatacgataa ttttccggaa atcagtaaag c
//
LOCUS       DAZFFI010000265         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFFI010000265 DAZFFI010000000
VERSION     DAZFFI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.267"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..621
                     /locus_tag="ACHJ12_005111"
     CDS             172..621
                     /locus_tag="ACHJ12_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992738.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMALARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            638..1054
                     /locus_tag="ACHJ12_005112"
     CDS             638..1054
                     /locus_tag="ACHJ12_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO4992739.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLRGALTSYIHTTRYLKAIVAGGPRY
                     GLNGQPCGEVTSDEKAVARGILKQRLEQMKQKNIQQ"
     gene            1074..1367
                     /locus_tag="ACHJ12_005113"
     CDS             1074..1367
                     /locus_tag="ACHJ12_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4992740.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWSEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            2204..2329
                     /locus_tag="ACHJ12_005114"
     CDS             2204..2329
                     /locus_tag="ACHJ12_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992741.1"
                     /translation="MTDRSPTEVIHGIIEEQRERQRRQEASQPDSVSAENQADVP"
BASE COUNT          738 a          602 c          698 g          451 t
ORIGIN      
        1 aaaaatctat gagaaatatg cagcaaacag caaaagagcc gcacgggcaa tcgctcacca
       61 tttcatgacg ctgtatgacc tgaaagcgga agatgcagaa aaagcggcat gtgatttatt
      121 cgatatgacc acagatatca tccatgagta ccggaacaag gagcgcagac catgacagca
      181 cagattgcag cttacgggcg gctggtggac gacccgcagg aaaaacagac cagcaaagga
      241 acaccaatgg cactggcgcg tatggcggta tctttgccat gcagtcaggc acaggacgga
      301 caggcgacgt tatggctatc agtcctggca tttggtaagc aggccgacgc gctggcgaga
      361 catgaaaaag gcgaccttat cagcgtggcg ggtaacatgc agatcagcca gtggaccgga
      421 cagaacggag aaatacggca gggctggcag gttatcgctg acagcgtaat cagtgcccgt
      481 tcggcgcgtc ctggcgggaa gcgggggcaa caggggaagg cgaccgaagc actggaaaga
      541 gcaaaacgac agaccggaca gcagggaaat cagccaccag cgggaggtga tgacccgttc
      601 tctgacgata ttccgttctg aatgacagga gcacaacatg aaccagcaga ccacacaaca
      661 ggaccagacc acacgcagac ccacggagcg cccatcatgg cgacacatga cgaagcggca
      721 gcgaaaaaac cgcaggagga taaaccgact ggctgagtac tggcctgaac tgtttggggc
      781 agagacgata aagcccctga aaacgggcat aatacgtgat gtgcttgagg acgctaaatc
      841 acgggaatta ccgattggtc atggcgtatt acgtggcgca ttaacctcat acatccacac
      901 gacgcgatac ctgaaagcca ttgtcgctgg tggccctcgg tatggactga acgggcagcc
      961 ttgcggggaa gtgacgtcgg atgaaaaagc cgtagcccgt gggatactga aacagcgact
     1021 ggagcagatg aagcagaaaa acatccagca gtaatcagcc ggaggtaaag ccagtgataa
     1081 cggacagcgt ggcacagaaa cttgaggagc gcggtttatg gcgacgtgcg gcgacccgat
     1141 ggtctgaggt tcttcttcac gcagaaacag acagggaacg cgaggaagct gccagacgtc
     1201 gggcgatatg tatcattaaa tcccgtcgta tgcctgagca gtttgtcacc tttggcgatg
     1261 tgaaaaaggc cgcagacaga acgctgaaag agatggggat taatccacag gatgaatggc
     1321 gaaattacag cttcagtgat gcaggcgacg atttagcgct atcgtgatac gcatctcatc
     1381 gccgtatttg ctcaattatc gcgattcgct ataatcacca ccgaaaggcc gttgatagtg
     1441 ttctcctcgg gcaactggag cccgactgaa tgaagccagc cagcggcttt tcggcactca
     1501 ttccggtgta tctcccatac atcgggatga gtgaacgacg aaaacatcac ggtgatttcg
     1561 tcatgccgcg ataagaatgc gcctctttgg gcaattgaag ccctctgtac agcgggttat
     1621 cgcggcaaag ttgtgataaa aacgcgcctc tttgggcaac tggagcccac tgtaaagcgg
     1681 cttatcacag ctggcggtaa cgggcgctca tctccgggca actgaagccc tctgtacaga
     1741 gacccgttac ggctgaccct gaatgatttc gccccgtgca attgaagtac ccagcggagt
     1801 tattcagggt tttttattgc ccgttaccag gcaccttcaa agccccttac aagtcgcatc
     1861 gaagggggtg aaaaatcccc gaaaaaatcc cctgaaaaat gcgcaaaaaa ctgcgcaaaa
     1921 aagtgcgcaa atcgatcata aaaccgatca caaaatcgat cacacagaca aacagagcaa
     1981 agccaggtat gacgcggctt gcagggggaa aggccaccgg aaaaagtgcg catttttctc
     2041 aaaaacgaaa cgcaacagga agcgcaaaac gaaaccgata cgtgatgatc gattttttgt
     2101 aagttactta aagtaaccta caaaaatgaa cgtttttaca gggcaggaaa cgagagtact
     2161 ggttaacatt cctggcataa cagcaactca cagggaggga cctatgaccg accgttcacc
     2221 aacagaggta attcacggga ttattgaaga gcagagagaa cgccagcgca gacaggaggc
     2281 ctcgcagccg gacagcgtga gcgctgaaaa tcaggctgat gtgccgtgat ggtgtcgaac
     2341 tgagtatctt taagcgactt cgtgactggt tttaaacgag tcgcaaaaaa agccggagaa
     2401 aaaccacgaa tctggggttt tttctaagca cgctgtacac ggtggaacat atggtggtca
     2461 ggagacggca ggagcaacgc cagaacgcg
//
LOCUS       DAZFFI010000266         2486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFFI010000266 DAZFFI010000000
VERSION     DAZFFI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.268"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            293..436
                     /locus_tag="ACHJ12_005115"
     CDS             293..436
                     /locus_tag="ACHJ12_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4992742.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            449..658
                     /gene="cspE"
                     /locus_tag="ACHJ12_005116"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             449..658
                     /gene="cspE"
                     /locus_tag="ACHJ12_005116"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4992743.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            824..1633
                     /gene="rlmA"
                     /locus_tag="ACHJ12_005117"
     CDS             824..1633
                     /gene="rlmA"
                     /locus_tag="ACHJ12_005117"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4992744.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1630..2196)
                     /gene="mntP"
                     /locus_tag="ACHJ12_005118"
     CDS             complement(1630..2196)
                     /gene="mntP"
                     /locus_tag="ACHJ12_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4992745.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          662 a          593 c          602 g          629 t
ORIGIN      
        1 catagcaaaa aatgtgatat tgcacgcact atgtaataac ttctcccact ggcctggaac
       61 aactgaactt attgaactat gttagaaaat acgccagttt aagtatctgc ctgaactggc
      121 aaggttaagc acaatgatat atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg
      181 gaagacgtat cgagatttgt gcgtctgatc gagacatgtt taaaaatggc ttgccataat
      241 taacgttgta tgtgataaca gatttcgggt taaacgaggt acagttctgt ttatgtgtgg
      301 cattttcagt aaagaagtcc tgagtaaaca cgttgacgtt gaataccgct tctctgccga
      361 gccttatatt ggtgcctcat gcagtaatgt gtcagtttta tctatgttat gcctgcgggc
      421 gaagaaaaca atctaaggaa tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt
      481 caacgagtct aaaggttttg gcttcattac tccggctgat ggcagcaaag atgtgttcgt
      541 acacttctcc gctatccagg gtaatggctt caaaactctg gctgaaggtc agaacgttga
      601 gttcgaaatt caggacggcc agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc
      661 gaatccactg atctgaagtg tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc
      721 gaggcttttt actatgcttt atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga
      781 ttcgcgttac acttgcggcc tttagtatcc tgccggagtt gtcatgtcgt tttcctgtcc
      841 actttgccat cagcctcttt cgcgtgaaaa aaacagctat atctgtcccc agcgacatca
      901 gtttgatatg gcgaaagaag ggtatgtcaa tctgctgccc gttcagcata aacggtctcg
      961 tgatccgggc gacagcgcgg aaatgataca agcacgccgc gcattcttag atgccggaca
     1021 ttatcagccg ctgcgtgatg caattgtcgc ccaactgcgg gaacggcttg atgaaaaggc
     1081 cacggcggtg ctggatattg gctgtggtga agggtattac acacacgcat ttgccgatgt
     1141 gttgcccgaa atcaccacgt ttggcctgga tgtctcgaag gtagcgataa aagcggcggc
     1201 gaaacgctat ccgcaggtca ctctttgtgt cgcttccagc caccgtttgc cgttttccga
     1261 taccagtatg gacgccataa tacgtattta cgcgccgtgt aaagcagaag aattagcacg
     1321 agtagtgaag cccggcggct gggtcattac tgccacgccg ggaccgcgac atttgatgga
     1381 gctgaagggg ctgatttaca atgaagtaca tcttcatgca cctcatgcag aacaactgga
     1441 aggttttaca ttacagcaga gtgatgagtt gtgttatccg atgcgtcttc gcggtgatga
     1501 agccgtcgca ttattgcaga tgacgccgtt tgcctggcgt gcgaagccag aagtctggca
     1561 aacactggca gcaaaagaag tgttcgactg ccagacggac tttaatattc acctctggca
     1621 gcgttcttat taaccgtgga agtgcgtcca gaggatctgg acgccgatgc cgatcagcac
     1681 cagcccgccg agaatttccg cttttttccc aataattgag ccgataaagc gaccaaccat
     1741 catccctaat gtcgacataa tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat
     1801 gttgacctgc aggaaagcaa gaccaacacc cacagccatg gcatccaggc tggtggcaat
     1861 cgcggtggtt accagtagcc agaaaccgtg tcgacggcgc ggctcttcat cttcatcatc
     1921 tgcgccacga aaaccctcaa taatcattcg cccgccgagg aatatcagca gcacaaacgc
     1981 aatccagtgg ttccattcaa ggacaaaccg gctggctaac atgcccattc cccagccgat
     2041 cagcggcgtc agggtttcga cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa
     2101 tttcggttta tggagggtgg cacctttacc gattgatgca gcaaatgcat ccatcgacat
     2161 accaaacgca agaagaacag tagcagtgat attcatgaca atgtcctgac cggggtatcc
     2221 ataaaacaca tcatcgcccc cagcaaacat gcatacatga acaacaagcc gtgatggttt
     2281 tcgcgttcat gcaggcatag cgattgatgt gcatatggtc tcgcctgact gaaagtattc
     2341 agtccgtacg tgccacgttt tgcaacgagt atgttgacac gtacatttcc ggaatctgga
     2401 aatcagctac tccccaaatg agggcgcaac cttaacatat tttgagaata taaaacaact
     2461 gttggaatat attatttcat tgcttt
//
LOCUS       DAZFFI010000267         2479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFFI010000267 DAZFFI010000000
VERSION     DAZFFI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.269"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            279..794
                     /gene="ehxC"
                     /locus_tag="ACHJ12_005119"
                     /gene_synonym="hlyC"
     CDS             279..794
                     /gene="ehxC"
                     /locus_tag="ACHJ12_005119"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO4992746.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            796..>2479
                     /gene="ehxA"
                     /locus_tag="ACHJ12_005120"
     CDS             796..>2479
                     /gene="ehxA"
                     /locus_tag="ACHJ12_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4992747.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNG"
BASE COUNT          775 a          411 c          554 g          739 t
ORIGIN      
        1 ttaataggtg ggttttactt tgctgcatcc tgtttgatat ttctttgatg agaagtttgt
       61 cgcagggtga ttcatctgaa tgttataata taacctatct aggtgtgcat aagaatacat
      121 agctaatgta ttactgtttc taatgttgtt ttttgtagga gggattctcc ttgcggtagc
      181 tttttgtttt tagatgctta ttgcttaaag aatataattt ttatgctttt atattgattt
      241 ctttacattt actgagtttt cttatttatt taaacattat gaaatctaat gcttttgacg
      301 tcatggggaa ggttgcatgg ctatgggcct gttctcctct gcataaaaaa tggccattgt
      361 ctgtttttgc tattaatgtc atacctgcta ttcagacaaa tcaatttgct ctcttaataa
      421 aggatgagct tcctgtagct ttttgtagct gggccagctt agatctggag tgtgaggtaa
      481 aatatataaa tgatgtaaca tctttgtatg caaaagactg gttgtcggga gaaagaaagt
      541 ggtttattga ctggatagct ccatttggac acaacatgga gttatataaa cacatgagga
      601 aaaagtatcc ttatgagtta ttcagagcaa taaggttaga tgagtacagt aaaacaggga
      661 aaatagctga atttcatgga ggggggatag ataaaaaact ggcatcaaaa atattcaggc
      721 aataccatca tgaacttatg tctgaagtaa aaaacagaca agattttaat tttaatattg
      781 agaaagaaaa ctaatatgac agtaaataaa ataaagaaca ttttcaacaa tgcgacatcg
      841 actacaaaat cagcattcaa tacagcatca tcaagcgtac gttccgctgg aaaaaaactc
      901 atattattaa tacctgataa ttatgaggct cagggcgtgg gtattaatga gttggtcaaa
      961 gctgctgatg atcttggaat agaaatacac cgtactgaac gagatgatac agcgattgca
     1021 aaccagtttt ttggtacagc agaaaaagtt gtaggattaa ctgaacgtgg tgttgcaata
     1081 ttcgcaccac aacttgacaa acttctgcag aagtatcaga aagttgggag taaaatagga
     1141 ggaaccgctg aaaatgtagg taataatctg ggaaaagccg gaacagttct ctcagcacta
     1201 cagaatttta cggggattgc tttatctggc atggctcttg atgaattgct gagaaaacaa
     1261 cgggaaggag aggatataag tcagaatgat attgccaaaa gtagtattga acttattaat
     1321 cagcttgtag atacagtatc aagtataaac agtaccgttg attcattttc tgagcagctt
     1381 aaccagcttg gctcattttt atccagtaaa cctcgcttaa gttctgttgg tggaaaatta
     1441 caaaatttac cgaacctggg ccccctgggg gatgggctgg atgttgtctc cggaattctt
     1501 tctgctgtat cagcaagctt tattctggga aacagtgacg cacatacagg aacaaaagct
     1561 gcagcgggta tcgaactgac aactcaggtt cttggaaatg ttggtaaagc tgtttcgcaa
     1621 tatattctgg ctcagagaat ggcacaggga ttatcgacaa cagctgcaag tgcgggtctg
     1681 atcacatcgg ctgttatgct ggctatcagt cctctttctt tcctggctat tgcagataaa
     1741 tttgagcgag ctaagcagct tgaatcatat tctgaacgat ttaaaaaatt gaattatgaa
     1801 ggggatgctt tactcgcagc ctttcataaa gaaaccggag ctatagatgc agccctgaca
     1861 acaataaata ctgtcctgag ttctgtatct gcgggagtta gtgccgcctc cagtgcatcc
     1921 ctcatagggg ccccgataag catgctggtg agtgcattaa ccggtacgat atctggcatt
     1981 ctggaagcat caaaacaggc tatgtttgag cacgttgcag ataaattcgc tgcccggatc
     2041 aatgaatggg aaaaggagca tggcaaaaat tattttgaga atggctatga cgcaagacat
     2101 gctgcgtttt tagaagattc tctgtctttg cttgctgatt tttctcgtca gcatgcagta
     2161 gaaagagctg tcgcaatcac ccagcaacat tgggatgaga agatcggtga acttgcaggt
     2221 ataacccgta atgctgatcg cagtcagagt ggtaaggcat atattaatta tctggagaat
     2281 ggagggcttt tagaggctca accgaaggag tttacacaac aagtttttga tcctcaaaaa
     2341 gggactatag acctttcaac aggtaatgta tcaagtgttt tgacatttgt aacaccaaca
     2401 tttaccccag gagaagaagt cagagaaaga aaacagagtg gtaaatatga atatatgaca
     2461 tctcttattg taaatggta
//
LOCUS       DAZFFI010000268         2453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFFI010000268 DAZFFI010000000
VERSION     DAZFFI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.270"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHJ12_005121"
     CDS             <1..474
                     /locus_tag="ACHJ12_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4992748.1"
                     /translation="APAPDILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVI
                     PGMKLSKEKCDWVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPST
                     FYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQISRRDQESALACWGIDR"
     gene            748..1443
                     /locus_tag="ACHJ12_005122"
     CDS             748..1443
                     /locus_tag="ACHJ12_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO4992749.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     LPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1538..1669
                     /locus_tag="ACHJ12_005123"
     CDS             1538..1669
                     /locus_tag="ACHJ12_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992750.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            1892..2077
                     /locus_tag="ACHJ12_005124"
     CDS             1892..2077
                     /locus_tag="ACHJ12_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4992751.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPATVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2163..2387
                     /locus_tag="ACHJ12_005125"
     CDS             2163..2387
                     /locus_tag="ACHJ12_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992752.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          635 a          538 c          676 g          604 t
ORIGIN      
        1 gcgcctgcgc ctgatattct cgaccagttt ctggatgaaa aagaaggcaa ccacaccaca
       61 gcataccgtg atggtgcggg tatctggacc atctgccgag gtgccatcat ggtggatggt
      121 aagcctgtga ttcctggcat gaagctgtcg aaggaaaaat gcgactgggt taacgctatc
      181 gaacgggata aggcgctggc gtgggtggag aaaaacatca gagtgccact gaccgaaccc
      241 cagaaagcgg ggatcgcgtc attctgcccg tataacattg gtcccggtaa gtgtttcccg
      301 tcgacgtttt ataaacgaat taatgcaggc gatcgcaggg gagcgtgtga ggcgattcgc
      361 tggtggatta aggacggtgg cagagactgc cgtattcgct caaataattg ctacggtcag
      421 atatcccgtc gtgaccagga gagtgcgctg gcgtgctggg gaatcgacag ataagcagaa
      481 tattttgctg aaaaatgacg ttggtcaacg cgggtggata acacgaaatc ctgcgaactg
      541 gcaaaaccta agtgaataaa gtcaggaaca ttgtttcacg cagaggcacc gtaatggtgc
      601 ctttgtcatt tctgcgcttc gcacaagcgt aaataaacca aagaaccttt caggatgagc
      661 cctggtggat aaccggcagt ggtctggtta accctctttg ggctggttat tcctgtgcgc
      721 agggttcatc actaaaagga atcaaccatg aaagagatga tttctgtcga tcgtgaaata
      781 tccatgagta gtctggattt tctgaataac attattaatc cagcccgggc agaagccgga
      841 gaagtccctc atgaaccgcg taagtttctt gcaaaaattg aggatgagct agagcttgat
      901 ggaaccggaa aaaaattccg gttaaacaat aaccagacaa gaacggcata ctatgatctg
      961 gattttgacc agatgatgct cgttggcatg agagagtcaa aggccgttcg tcgttctgtg
     1021 ctggcaaggc tcaaagcgat gcatggtatt cagctccccc agactttacc tgaggcgttg
     1081 cgatttgcgg caaaactggc agaacagaaa gcagagctgg aaaatcaact ggcaatagca
     1141 gcaccgaagg ctgaatttgt tgataactat gttgaagcat ctggtctgat gggattccgg
     1201 gaagttgcta agttactcgg tatcaaagaa accgatttcc ggctgttttt gttggagaac
     1261 ggaataatgt atcgcctcgc tggaaaaatg acgccttact cgcatcacct ggatgcgggg
     1321 cggtttagcg tgaaaacggg cgaggcgggc aatggtcatg cttttacgca ggttaaattc
     1381 accccaaaag gtgttcagtg gattgctggt ctgcttgctg catggagagc taccgcagca
     1441 tgaagatgat aaaaatggac tggaaatttt tgctggtctg gctgattccg tttttatggg
     1501 tggttgcccg gttaattact gctattaaga ggtaaagatg tcagacaaac tcataatgct
     1561 ggcgaagggc ctttgtgtaa tcgtcggtat ttcattttca ctaatgctgg ttgctctttt
     1621 tctttccata acctggatgg cgttgacttc ggcagggctg gtggggtgag cataaaccga
     1681 atgctttccg cgtttaccgt tattctgctg gtggtctgtg gtgcgcttag tctggggctg
     1741 aatcattacc gcgatagcgc catcacctac aaagcgcagc gcgataaaaa agccagagag
     1801 ctggagctgg caaacgcaac cattactgat atgcaggtgc gccagctcga tgttgctgcg
     1861 ctcgatgcaa aatactcgag ggaattagcc gatgcgagag ctgaaaatga aactctgcgt
     1921 gctgatgttg ccgctggtcg taagcgcctg cggatcaacg ccacctgcca cggtaccgtg
     1981 cgtgaagcca ccagcacctc cggcgtggat aatgcaaccg gcccccgact ggcagacacc
     2041 gctgaacggg attatttcat cctcagagaa cggttgatga caatgcagaa gcagctggaa
     2101 ggggcgaagg aatatatccg cactcagtgt attaactagt atttttgtta tccggagaat
     2161 gcatgaagaa attactggta accgtagaac cttttcaggg aacaattccg ttccgtattt
     2221 tgcagcgtgg tcgtgttctt gttgaaggtt cgttcagtgg taaatgtacg caattacact
     2281 cccggacctt tcaggtgaat gccacgaatg aagagctaac cgttgagtgt acgatgaatg
     2341 ccgctaaatg ccgcatggta tccgctgcat tacagccagt gtgttgagcg accttattat
     2401 ccatgcgcgg tattgtcgcc gtattcccgt attaacagag accgcagccc gac
//
LOCUS       DAZFFI010000269         2443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFFI010000269 DAZFFI010000000
VERSION     DAZFFI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.271"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..396)
                     /gene="hokD"
                     /locus_tag="ACHJ12_005126"
     CDS             complement(241..396)
                     /gene="hokD"
                     /locus_tag="ACHJ12_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4992753.1"
                     /translation="MKQQKAMLIALIVICLTVILTALVTRKNLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(687..1028)
                     /locus_tag="ACHJ12_005127"
     CDS             complement(687..1028)
                     /locus_tag="ACHJ12_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992754.1"
                     /translation="MPDDGREQFEALIRFYADDKNHETLLLRANEGMNYRDPNVDLAW
                     IFWKSSREHILPGKSDNNPASGDQVSELTMWVKRLSHSLKSVNKSSKLPDKAMDYLKR
                     NGLISEEDILR"
     gene            complement(1020..1169)
                     /locus_tag="ACHJ12_005128"
                     /pseudo
     CDS             complement(1020..1169)
                     /locus_tag="ACHJ12_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1406..1537)
                     /locus_tag="ACHJ12_005129"
                     /pseudo
     CDS             complement(1406..1537)
                     /locus_tag="ACHJ12_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(1539..1883)
                     /locus_tag="ACHJ12_005130"
     CDS             complement(1539..1883)
                     /locus_tag="ACHJ12_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4992755.1"
                     /translation="MSEIDYQALRKAAQNYQSTLAWYQENPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEVKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(1870..2118)
                     /locus_tag="ACHJ12_005131"
     CDS             complement(1870..2118)
                     /locus_tag="ACHJ12_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992756.1"
                     /translation="MFKQNEKSIAQIAEYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQIRKGGTSERD"
     gene            complement(2111..2314)
                     /locus_tag="ACHJ12_005132"
     CDS             complement(2111..2314)
                     /locus_tag="ACHJ12_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992757.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTKRMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPIEVKHV"
BASE COUNT          563 a          645 c          515 g          720 t
ORIGIN      
        1 agataatgcc gacatttata aattggcaga tggttctact atcattgatt tttattccct
       61 tcgaggagtc ttagacccca ccaatgggaa aagcaagagg attcagggaa ttaaatcatg
      121 tattagcact aaataatcat cagttcgaac caagaaacag ccaaatagat tttatctcag
      181 gtgcgttgag gatgcctgcc acatcagagg tggcgaggga tttctccctc gccgggtctc
      241 ttactcctca ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggattcg
      301 tacctcgcag aggtttttcc tcgttaccag tgccgtcaat atgacggtta aacagatgac
      361 gatcagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcag
      421 tacgtaagag gctaacctac atgtgcaaag catgaaattg gcctcagatt aatgttaagc
      481 gtcctgcaag acgcgtaatg ttaactgggg cttttctatg tctgccttac ggcggcatgc
      541 ccgaggcaga cagcctcaag cacccgcagc aattctactt aactcttctt tccccgcaaa
      601 ccgtttttat ccccagcggc aaatcgaata caccaccagc gccaccgcca ttgcaattcc
      661 taccgttatg aacgcttcag gccaggtcat cgtaaaatat cctcctcgct tatcagtccg
      721 tttcgcttca ggtagtccat cgctttatcc ggtagtttgc ttgatttatt aacgcttttt
      781 aaggaatgag ataaccgctt cacccacatt gttaattcac ttacctgatc gcctgatgct
      841 ggattattgt cgctttttcc cggcaatatg tgctctcgac tggatttcca gaatatccac
      901 gccaaatcca catttggatc ccggtaattc atcccttcgt tagcacgaag caacaatgtt
      961 tcatggtttt tatcatccgc atagaacctg ataagcgctt cgaactgttc gcgaccgtca
     1021 tcaggaatta cctgaaagtt gccagcctga cgcatagcta tttccatgat ctcaaccata
     1081 tcccctggtg gaattttaca atgctgacca atatgcctct gctgcctggc atattcgagg
     1141 atgtgctcca gtttggttcg atcaatcatg atttatctcc attaagcatt gcagcacggc
     1201 aatcgttcca tccttctgca taatcactgt gcgcaagagg ccaacctctt atgtattcac
     1261 gcggcaactt atcaggcact ggcggggcgg cgtatacttc aattgtccca ttatcaatag
     1321 gccactcccc atccttgatg tagtcacttg tgccatcgac ctgctgttct gcaatgtgga
     1381 aagcacctat tggttttgct tcctgtgatg ccagtgcaat tttaaatgcg gtaagcatgt
     1441 tgctttgatc ttcatcgagt ccgaacggta tttcatcccg tgctgactca atgctggtaa
     1501 tcgttttctg tagccattct ttggtaatag tggtcatctc actctccttc tatgcgaata
     1561 ccagcagcag tcagtgctgc attgacctca tcagcgtagt agtacgttaa tccacttgat
     1621 gatttagcca gcttgaatgg ttctggtaac ttcactgtgc gggactcctg ctcgtcgata
     1681 cgctgtttta cttcctccag ttcatccagc aaatcagcga taatatctac ttcacgatga
     1741 cggatttcac gcttaaacgc agccaaagct gcatcacaat cctgctcagc atttggactg
     1801 tctggatttt cctgatacca cgccagcgtc gattgatagt tttgtgctgc cttacgcaac
     1861 gcctgatagt caatctcgct cactggtgcc tcctttacgg atctgcgctg cgatgcacga
     1921 aaaaaaagac tctcgcgtat gacagttaag agctggtgcg aacgccgcgt taagaacggc
     1981 ggcatcacag ccgtcatcaa tatagagcgc aatttttttc tccaggcgcg ctttggcttc
     2041 ctgcaactgc ataccccggc acgcacgcgg gatatactca gcaatttgag cgatagattt
     2101 ttcgttctgt ttaaacatgc ttcacctcga taggcttgat ggtatcgatc agcagtcggc
     2161 ggcgagtatt ttctgcaaag tggcggcgtc cggtttcttt gtggtaaaac tcgttttttc
     2221 cgacgaccca catccgcttt gtctggtgca gtttttttac ctgcggaccg tctttggtga
     2281 tcacgatgcc ggtatgggtt tttacgattg tcatgccacc acctcttcga atttcaattc
     2341 caattgttca ccccagattt cacatgactc tgaacacgag ccggtatcga attgcctggc
     2401 ctgcaccatc gcctgatagt taatcttcat cgtcctcacc cca
//
LOCUS       DAZFFI010000270         2408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFFI010000270 DAZFFI010000000
VERSION     DAZFFI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.272"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1808
                     /locus_tag="ACHJ12_005133"
     CDS             <1..1808
                     /locus_tag="ACHJ12_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFO4992758.1"
                     /translation="ILHWDFSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGI
                     ALEVWPGSEFQAETQQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQL
                     VMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMSTLINVQWQSGLFDHL
                     LRLPLAFFERRKLGDIQSRFDSLDTLRATFTTSVIGLIMDSIMVVGVFVMMLLYGGYL
                     TWIVLCFTTIYIFIRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGI
                     RGAYWLNMKIDAINSGIKLTRMDLLFGGINTFVTACDQVVILWLGAGLVIDNQMTIGM
                     FVAFSSFRGQFSERVTSLTSFLLQLKIMSLHNERIADIALHEKEEKKHEIEIVAHMGP
                     ISLETNDLSYRYDSQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDS
                     GRVLINGIDIRQMGINNYHRIIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARA
                     SHIHDVIMNMPMGYETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSE
                     SEHFVNVAIKNMNITRVIIAHRETTLKTVDRVISI"
     gene            complement(1995..2312)
                     /locus_tag="ACHJ12_005134"
     CDS             complement(1995..2312)
                     /locus_tag="ACHJ12_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HFO4992759.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVIGGAFGAGLC
                     GPVCAGAFAVGSSAAVAALYDAAGNSNSAKQKPEGLPPEAWNYAEGRMCNWSPNNLSD
                     VCL"
BASE COUNT          688 a          465 c          560 g          695 t
ORIGIN      
        1 gtattctcca ctgggatttc agtcacttcg tcgtgctggt cagcgtaaag cgtaaccgtt
       61 atgtactgca tgatccggcc agaggcagaa gatatctcgg tcgggaggaa atgagccggt
      121 attttacggg tattgcactt gaggtctggc ccggaagtga attccaggcg gaaacccagc
      181 agacccgcat aagtctccgt tcactgatta acagtattta cggtattaaa agaacactgg
      241 cgaaaatttt ctgtctgtca gttgtaattg aagcaatcaa tctggtaatg ccggtgggaa
      301 ctcagctggt tatggatcat gcgattccgg cgggggacag agggctgcta acgcttattt
      361 ctgctggcct gatgttcttt atattgctca gggccgcggt gagtatgctg cgtgcatggt
      421 cctcactggt tatgagcacg ctcatcaatg tacagtggca gtcggggctg ttcgatcatc
      481 ttctcagact accgctggcg ttttttgaac gccgaaaatt aggtgatatc cagtcacgtt
      541 ttgactccct tgacacattg agggccacat ttaccaccag tgtgatcggg ttaataatgg
      601 acagcattat ggttgtcggt gtttttgtga tgatgctgtt atacggagga tatctcacct
      661 ggatagttct ctgctttacc acaatttaca tttttattcg tctggtgaca tacggcaatt
      721 accgacagat atcagaagaa tgtcttgtca gggaggcccg tgccgcctcc tattttatgg
      781 aaacattata tggtattgcc acggtaaaaa tccaggggat ggtcggaatt cggggagcat
      841 actggcttaa tatgaaaata gatgcgataa attcgggtat taagctaacc aggatggatt
      901 tgctcttcgg aggaataaat acctttgtta ccgcctgtga tcaggttgta attttatggc
      961 tgggagctgg ccttgtgatc gataatcaga tgacaatagg aatgtttgta gcgtttagtt
     1021 cttttcgtgg gcagttttcg gaaagagtta cctctctgac cagttttctt cttcagctaa
     1081 aaataatgag tttacacaat gagcgcattg cagatattgc attacatgaa aaggaggaaa
     1141 agaaacatga aattgaaatc gttgctcata tggggccaat atccctggaa accaatgatt
     1201 taagctaccg gtatgacagt cagtcagctc cgatattcag tgctctgagt ttatcagtag
     1261 ctccggggga aagtgtggct ataactggtg cttccggtgc gggaaaaacc acattaatga
     1321 aagtactatg tggactattt gaacctgata gcgggagggt actgataaat ggtatagata
     1381 tacgccaaat gggaataaat aattatcacc ggataatagc ctgtgttatg caggatgacc
     1441 ggctattttc aggctcaatt cgtgaaaata tctgtggttt tgcagaggaa atggatgaag
     1501 agtggatggt agaatgtgcc agagcaagtc atattcatga tgttataatg aatatgccaa
     1561 tgggatatga aacattaata ggtgaacttg gggaaggtct ttctggcggt caaaaacagc
     1621 gtatatttat tgcacgagcc ttataccgga aaccaggaat attatttatg gatgaggcaa
     1681 ccagtgctct tgattcagag agtgaacatt tcgtgaatgt tgccataaaa aacatgaata
     1741 taaccagggt aattattgca cacagagaaa caacgttgaa aactgttgac agagttattt
     1801 ccatttaaaa cctactgaca taaccataga ggaattgcaa ctgtatgagg aatatatctt
     1861 cctgtgataa ttcctcgtta tgttcagttg tcagttggag gaggaatgga agatagacaa
     1921 cccaccagga agcatatgta tcattctgtg tggattgtcc aataacagtt ctttcctata
     1981 ttaaaaaata ctatttataa acaaacatca ctaagattat ttggactcca attacacatt
     2041 ctaccttcag cgtagttcca tgcttctgga ggtagtcctt ctggtttttg tttcgctgag
     2101 ttggaatttc ctgctgcatc atacaaagca gcaacagcgg cagaagatcc aacagcgaag
     2161 gcaccagcac aaacagggcc gcacagacca gctccgaatg caccgccaat cactcctgca
     2221 ccgtttgttg ctacagtttt cccgacatca acccaattga catcaccagc accagagaca
     2281 ttattcattt catttaacgt tatttctctc atatatcatt tacccctatt aaaaaaaata
     2341 gatatcatta atgcaatggc gataccgcta acaacagatt gaacaaggtt gctagaattt
     2401 agttgttt
//
LOCUS       DAZFFI010000271         2408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFFI010000271 DAZFFI010000000
VERSION     DAZFFI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.273"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2262
                     /locus_tag="ACHJ12_005135"
     CDS             <1..2262
                     /locus_tag="ACHJ12_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992760.1"
                     /translation="VLTNENGSQSTFLYDPVDRLAEQRGFDGRTQRYRYSATGQLVHS
                     EDEGLITLWHYDASDRITHRTVNGDPAEQWQYDDHGWLTEISHTSEGHRVAVHYGYDD
                     KGRLTGERQTVENPETGEMLWEHETKHAYSEQGLANRQEPDGLPPVEWLTYGSGYLAG
                     MKLGGTPLVEYTRDRLHRETARSFGGEACELATAWNTSGQLQSRHLNLPQLDRDYDWN
                     DNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPD
                     STLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDLIPEGVIRMHDERTHHYHYDSQHRLV
                     FYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDRL
                     TTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAV
                     MLGRLERELRAGAVSAESEAWLAQCGLTVEQMESQMEAEYIPERRLHLYHCDHRGLPQ
                     ALISPEGETAWCGEYDEWGNQLNEENPHHLYQPYRLPGQQYDEESGLYYNRHRYYDPL
                     QGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGLERVISVYGPPAPDRAGAETPLVLT
                     DMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGAGDPYTGEVVGCETNESGISAAYGT
                     TKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTMGCTRAQNEDVDELCKKVTSWMYSH
                     PGERIRYERFKTR"
BASE COUNT          597 a          595 c          785 g          431 t
ORIGIN      
        1 gtgctgacca acgagaacgg cagccagtcc acgttcctgt atgacccggt ggaccgcctg
       61 gcagaacagc gtggctttga cggacggaca cagcgttacc ggtacagcgc cacagggcag
      121 cttgtccaca gtgaagacga gggacttatc accctctggc actacgatgc gtcggaccgc
      181 atcacgcacc ggacggtgaa cggtgacccg gcggagcagt ggcagtatga cgaccacggc
      241 tggctgacag aaatcagcca taccagtgaa ggccatcgtg tggcggtcca ctacggctat
      301 gacgataaag gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggag
      361 atgctgtggg agcatgagac gaagcacgcg tacagtgaac aggggctggc gaaccgtcag
      421 gagccggacg gtctgccgcc ggtggagtgg ctgacgtatg gcagtggtta tcttgcgggg
      481 atgaagctgg gtggtacgcc actggtcgag tacacgcggg accggctgca ccgtgagacg
      541 gcccgcagct tcggcgggga ggcctgtgaa cttgccaccg cctggaatac cagcggccag
      601 ctacagagca ggcacctgaa ccttccgcag cttgaccgtg actacgactg gaacgacaac
      661 ggacagctaa tccgcatcag cggcccgcag gagagccggg agtaccgtta cagcgacacg
      721 ggaaggctga cgggcgtcca caccacggca gcgaacctgg atatcgatat cccgtatgcg
      781 acggacccgg caggaaaccg gctgccggac ccggaactgc acccggacag cacgctcacg
      841 gcgtggccgg ataaccgcat cgcggaagat gcgcactatg tctaccgcca cgatgaatac
      901 ggcaggctgg cggagaagac ggacctcatc ccggaagggg ttatccggat gcacgacgag
      961 cggacccacc actaccatta cgacagccag caccgtctgg tgttctacac gcggatacag
     1021 cacggcgaac cacaggtgga gagccggtac ctctatgacc cgctgggccg ccggacggga
     1081 aaacgggtat ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg
     1141 gaggagacct ggtacgggtg ggacggtgac aggctgacca ctgtacagac ccaacagaca
     1201 agaatccaga cggtatacca gccgggaagc ttcacgccgc tcctgagaat cgaaacagag
     1261 aatggcgaac aggcgaaggc gcggcaccgg agcctggcgg aggtgttgca ggaggacacg
     1321 ggggtgacgc ttccggcgga gctggcggtg atgctgggga ggctggagcg ggaactgcgt
     1381 gcgggagcgg tgagtgcgga gagtgaggca tggcttgcgc agtgcgggct gacggtggag
     1441 cagatggaaa gccagatgga ggcggaatac atcccggagc ggaggctgca tctttaccac
     1501 tgtgaccacc ggggactgcc gcaggcgctc atcagcccgg aaggggaaac ggcgtggtgc
     1561 ggggagtatg acgagtgggg gaaccagctt aatgaggaga acccgcatca cctgtaccag
     1621 ccgtaccgtt tgccggggca gcagtatgat gaggagtcgg gactgtatta caaccgtcac
     1681 cggtactacg atccgttgca ggggcggtat atcacccagg atccgatagg gttgaagggg
     1741 ggaataaatc tatacacgta tccactggca ccaatacgtt acactgatcc actggggtta
     1801 gaacgagtta taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct
     1861 cttgttctta ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat
     1921 tcaatgactt ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat
     1981 ccatatacag gtgaagtcgt tggttgtgag acaaatgaat caggaatatc ggcagcatat
     2041 ggcacaacga agatctatac tacagatact agagcaagat ggctacatgg tgggggatca
     2101 tcgttgcgcg atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt
     2161 gcacagaatg aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat
     2221 cctggagaga gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt
     2281 gtgcattcta attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt
     2341 acccgctcct gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa
     2401 aaaagttg
//
LOCUS       DAZFFI010000272         2385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFFI010000272 DAZFFI010000000
VERSION     DAZFFI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.274"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..675
                     /locus_tag="ACHJ12_005136"
     CDS             <1..675
                     /locus_tag="ACHJ12_005136"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4992761.1"
                     /translation="PLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGR
                     AVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGL
                     IVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNV
                     DLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIMDFM
                     TARGYH"
     gene            complement(724..2223)
                     /gene="pitB"
                     /locus_tag="ACHJ12_005137"
     CDS             complement(724..2223)
                     /gene="pitB"
                     /locus_tag="ACHJ12_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO4992762.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPHLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            2160..>2385
                     /locus_tag="ACHJ12_005138"
     CDS             2160..>2385
                     /locus_tag="ACHJ12_005138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992763.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFHGVGK
                     VKYHLLTIKRVLFLVYQTISIHSKSLEIIKT"
BASE COUNT          648 a          614 c          578 g          545 t
ORIGIN      
        1 ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctt
       61 attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga
      121 aatgtcggtc gtgcagtgac aaaattaccg ttgatgggaa aatccttaca taaaacaatt
      181 gaacgaaatc aggtgaaaac agcaaaaaaa ttgccaggac cagttccggc gctggtaatt
      241 acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc
      301 gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc ttacgatggt
      361 acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc aagccaggaa
      421 cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt
      481 gaccttgaag ttgcctgcgc gcgtaagcct gaccacaaaa gggagtcgct ggcaagaaag
      541 atagccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat cgatgccaat
      601 cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttatggattt tatgaccgcg
      661 cgtggttatc actagccaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga
      721 aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa tcgccgccgg
      781 taaagtaaat acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc
      841 gtccaccacc atcgtccctg caactgcaga cgagaggacg tgtgttgtgg agacgggcat
      901 cccaatataa ctggcaagac cgatagacac tgccgccgtc atttgtgccg ccatgccttg
      961 cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca
     1021 gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc acaccggagc
     1081 gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct ggtcttcttt
     1141 actgacgcct ggcagtttcg ccagcttcgc ggaggtatct gagatgcaca gcataatgcg
     1201 gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat tgcctggcag
     1261 catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt
     1321 tacttgcgcg ccatcagtcg atgccgcagg caatggaggt tccatcgcaa tcaacttctg
     1381 cggcagttca ggatgctgtt gcagatagtg ttcgaagttg gagacggcat cgcgagtacg
     1441 ggtaatttca tagccggacg cgttcatatt gacgacgaag ccagcagggg caatccccac
     1501 cagtaccagc atcaccaggc cgatcccttt ctgcccgtcg ttcgcgccgt gcgaaaacgc
     1561 cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc
     1621 ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct
     1681 ccagaaacgg cgcagcagga aaatcagacc acccgcaatg accaggccga cgatagggga
     1741 aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat ccatcaccga
     1801 tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcgccaa tcaaggtgtg
     1861 cgaactggag gccggtaaac cgaagaacca cgtccccagg ttccatataa tcgccgccag
     1921 cagcatggaa aagaccatcg ccagaccgtg ggttgacccc atattcagca acaaatcggt
     1981 tggcaacata tggacgatgg cataggcgac gctaagcccg ccaagcaaca caccaaaaaa
     2041 gttaaaaaac gccgccatta ctaccgcaag atgcggctgc atggcacgag tataaataac
     2101 ggttgccacc gcgttcgccg tgtcgtgaaa accattgatt gcttcgtaga acaacacaaa
     2161 tgccagagca agtaataaca aaagccccgt atatatatca aggccaacaa ataaatttag
     2221 catatattag attacgccat tttgaattta cgaacggacg cattatcagt gactttcacg
     2281 gcgtgggcaa agtgaaatat catcttttaa cgataaaaag agtgttattt cttgtgtatc
     2341 agacaataag cattcattca aaatctttag aaataatcaa aacac
//
LOCUS       DAZFFI010000273         2372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFFI010000273 DAZFFI010000000
VERSION     DAZFFI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.275"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..1498)
                     /gene="pykF"
                     /locus_tag="ACHJ12_005139"
     CDS             complement(86..1498)
                     /gene="pykF"
                     /locus_tag="ACHJ12_005139"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4992764.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1506..1700)
                     /locus_tag="ACHJ12_005140"
     CDS             complement(1506..1700)
                     /locus_tag="ACHJ12_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992765.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2055..2264
                     /gene="fumD"
                     /locus_tag="ACHJ12_005141"
     CDS             2055..2264
                     /gene="fumD"
                     /locus_tag="ACHJ12_005141"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4992766.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          625 a          515 c          575 g          657 t
ORIGIN      
        1 ttttttattg cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata
       61 tacaaattaa ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc
      121 cgctcggtac cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc
      181 tctgcagagc cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa
      241 ccagctgcgg cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg
      301 tcagtgccag gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc
      361 cctgagtagc aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac
      421 ggcataccgc ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt
      481 tcatcacgcg gtcggtacgt tcgcaaatgg tcgccatgat agaaaccgct tccagcgggt
      541 atttaccttt tgcggattca ccagacagca tcactgcatc agtaccgtcg aggatggcgt
      601 ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca
      661 tctgggtcgc agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct
      721 gggcgaagat aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga
      781 tgccgtcaga ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt
      841 tggagatgat gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa
      901 cgtcagaacg cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa
      961 agatcaggtc ctgtttgtct ttttcagcca gtgctggcag agcaatggaa acgccaggca
     1021 ggttcacacc tttgttttcg cccaggtcac cgttgttcag cactttacag ataactttgt
     1081 taccttcaat ggcggtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc
     1141 caactgacag gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga
     1201 taacagattt atcagtggtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac
     1261 cgccttccag tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg
     1321 ttttaccagt tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat
     1381 agtcaccatg agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta
     1441 acatctcttc agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga
     1501 cagtcttagt ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg
     1561 aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa
     1621 aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt
     1681 cgttgagttc tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata
     1741 cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta
     1801 gacaataaat tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc
     1861 tgtttgcgca acgtctggtg ccaaagttgt cagtgtgagg ataagtgaaa gttcatccgg
     1921 tgtataaata tatcatcgct caattgctat agttaaaata atttatcgcg ttatttgtct
     1981 gattttgttt gatttttgtt taattggtta gattgatata tgctgatagt tggcatgata
     2041 aataggtata aagaatggga aatcggacaa aagaggatga actgtaccga gaaatgtgca
     2101 gagttgtcgg taaagttgtg ctggaaatgc gcgatctggg gcaggaacca aagcatattg
     2161 ttatcgcagg cgtattacgt acagcattag ccaataaacg cattcagcgc agtgagctgg
     2221 aaaagcaggc gatggaaacc gtcattaacg cactggtaaa atgaccattc aggatgaggc
     2281 ggtaagcttc atcctgatat tcttgcttca gttgcggtcc agttaatcat tctaaataac
     2341 aactgctgaa attatacgaa tcgcggtatg ta
//
LOCUS       DAZFFI010000274         2358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFFI010000274 DAZFFI010000000
VERSION     DAZFFI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.276"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..638
                     /locus_tag="ACHJ12_005142"
     CDS             135..638
                     /locus_tag="ACHJ12_005142"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4992767.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(684..1100)
                     /gene="rraB"
                     /locus_tag="ACHJ12_005143"
     CDS             complement(684..1100)
                     /gene="rraB"
                     /locus_tag="ACHJ12_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4992768.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1262..2266
                     /gene="argF"
                     /locus_tag="ACHJ12_005144"
     CDS             1262..2266
                     /gene="argF"
                     /locus_tag="ACHJ12_005144"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4992769.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          610 a          557 c          580 g          611 t
ORIGIN      
        1 gttctgccac gttacaatac tcttatcagt aataggctgg caaaaacttt attactttat
       61 aagtcgcttg cgcacgtaag aagagagtgg tattaaacaa atgtataatt actttatttt
      121 tattgggtta aactatgaat aacattgcgc cgcaatcacc tgtaatgcgt cgcctgacgc
      181 agcaggataa tcccgccatc gcccgcgtca ttcgtcaggt atccgccgaa tacggtctta
      241 ccgctgataa aggctacacc gtcgccgatc cgaacctgga cgagttgtat caagtgtata
      301 gtcagcctgg ccatgcatat tgggtggttg agttcgatgg tgaagtggtc ggcggcggtg
      361 ggattgcgcc attagcaggt agtgaatcgg acatttgcga actgcaaaag atgtattttc
      421 ttccggctat tcgcggtaaa gggctggcga aaaaactggc cttaatggcg atggagcagg
      481 cacgagagat gggcttcaaa cgctgctatc tggaaacgac cgcttttttg aaagaagcga
      541 ttggtttgta tgaacattta ggttttcaac atatcgacta tgcacttggc tgcacgggcc
      601 atgtagattg tgaagtgcgg atgctacgcg aactctaaaa aaatgcccgc tagtgtgtgc
      661 tatcgggcat tttaatcaat taattagtgg cgaaccccgt catcgtcttc atcgacaaaa
      721 tcttcatcgt cgccgtcttc gccattggga tcttcaaagt aagtgcccca accgtcgtac
      781 tccacgtcaa atttctccgc cagcgtcatc agttgttcaa cctgggcatc gatcagatcg
      841 gcattcaacg cgcactcgct gaggatgtcg cagcaaatca cgatatcacc atcttcaact
      901 tccagctctt ctggatcggt cacttcgtaa ccgagtttaa acgcttcaac tgctgctttt
      961 tccagggttt ccagatcgtc tgcggaaaga tggtgttcga tggtgtacag cgcgtccgga
     1021 tcgctgccat cttccagtaa ttcttcaata atcaaacgtg tttcttcacg ctgttcttcc
     1081 agttgttccg ggtttgccat ggctcattcc tcttaatgtc ctgccgatac tcttattgtc
     1141 acacaccact gacattgcct ccacctttgt gacaaagatt tatgctttag acttgcaaat
     1201 gaataatcat ccatataaat tgaattttaa ttcattaagg cgttagccac aggagggatc
     1261 tatgtccagg ttttatcata agcatttcct gaaattactc gatttcacgc cagctgaact
     1321 caacagcctg ctgcagttag ccgcgaaact gaaagccgat aagaaaagcg gcaaagaaga
     1381 agccaaactc actggtaaaa acatcgcgct catcttcgaa aaagactcga cccgtacccg
     1441 atgctctttc gaagttgccg catatgacca gggtgctcgc gttacttatc tcggcccaag
     1501 cggcagtcag attggtcata aagagtcgat taaagacact gcccgcgtgc tgggtcgcat
     1561 gtatgacggt attcagtatc gcggctatgg tcaggagatt gtcgaaacac tggcggaata
     1621 cgctggcgtg ccggtatgga acggcctgac caatgagttt caccccacgc agttgctggc
     1681 ggatctcctc accatgcagg agcatttacc cggtaaagca ttcaacgaaa tgacgctggt
     1741 ctatgcaggt gacgcacgta acaacatggg caattcgatg ctcgaagctg cggcgcttac
     1801 cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg ccggaagctg cgctggttac
     1861 ggaatgccgc gccctggcac agcaaaatgg cgggaatatt acgctgacgg aagatgtagc
     1921 caaaggagtt gaaggtgctg actttatcta taccgatgta tgggtgtcga tgggggaagc
     1981 aaaagagaaa tgggcagaac ggattgcatt actgcgtgat tatcaggtga acagcaagat
     2041 gatgcagttg accggtaacc cggaggtcaa attcctccat tgcctgcccg cgtttcatga
     2101 cgaccagacg acgcttggta agaaaatggc ggaagagttt ggcctacatg gcggaatgga
     2161 agtgactgat gaggtcttcg aatctgccgc cagcattgtt tttgatcagg cggaaaaccg
     2221 catgcatacc atcaaagcgg tgatggtcgc gacgctcagt aaataattga tatcgctata
     2281 aagatgccgg gaaatacaaa aacatcccgg catcacatta ttttatgcag taatagttca
     2341 attattccat ccatattt
//
LOCUS       DAZFFI010000275         2356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFFI010000275 DAZFFI010000000
VERSION     DAZFFI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.277"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2356)
                     /locus_tag="ACHJ12_005145"
     CDS             complement(<1..>2356)
                     /locus_tag="ACHJ12_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992770.1"
                     /translation="QLTSVTYPDGLRSSRKYDRQGRLAEEISRNGNITRWFYDSSRSG
                     LPCAVEDGTGVRRRITRNRYGQLQAFTDCSGYATRYEYDRYGQQIAIHREEGISTYSS
                     YNPRGQLVSQKDAQGRETRYEYSAAGDLTATISPDGKRSTIEYDKRGRPVSVTEGGLT
                     RSMGYDAAGRITTLTNENGSQSTFLYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSED
                     EGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRVAVHYGYDDKG
                     RLTGERQTVENPETGEILWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMK
                     LGGTPLVEYTRDRLHRETVRSFGGEACELATAWNTSGQLQSRHLNLPQLDCDYTWNDN
                     GQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDST
                     LTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFY
                     TRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTT
                     IQTGTTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAVML
                     GRLERELRAGAVSAESEAWLAQCGLTAEQMAAQMEAEYIPERKLHLYHCDHRGLPQAL
                     ISPEGETAWCGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGLYYNRNRYYDPLQG
                     RYITQDPIGLKGGWNLYAYPLNPVQRGDPLGLSGETLVLGSATTV"
BASE COUNT          360 a          816 c          642 g          538 t
ORIGIN      
        1 actgttgtgg cagaaccaag aaccagtgtc tcaccagaca acccaagagg atccccccgc
       61 tgcaccgggt tcagcggata cgcatacagg ttccatcccc ccttcagccc aatgggatcc
      121 tgggtgatat agcgtccctg caacggatcg tagtaccggt tccggttata gtacagcccc
      181 gactcctcat catactgctg ccccggcaga cggagtgact gttgaaggtg ctgcgcactg
      241 gtttcgccca gcaggtttcc ccattcgtca tactccccgc accacgccgt ttccccttcc
      301 gggctgataa gcgcctgcgg cagtccccgg tggtcacagt ggtaaagatg aagtttcctc
      361 tccgggatgt attccgcctc catctgcgcg gccatctgtt ccgccgtcag cccgcactgc
      421 gcaagccatg cctcactctc cgcactcacc gctcccgcgc gcagttcccg ctccagcctc
      481 cccagcatca ccgccagctc cgccggtagc gtcacacccg tgtcctcctg caacacctcc
      541 gccaggctac ggtgccgcgc cttcgcctgt tcgccattct ctgtttcgat tctcaggagc
      601 ggcgtgaagc tccccggctg gtacaccgtc tggatacgcg tggtgccggt ctgtatcgtc
      661 gtcagcctgt caccgtccca cccgtaccat gtcacctccg gtttacgcga cagcgacatc
      721 caccccgtca ggtcacgctc ccgccgccat acccgtttcg ccattcgccg tcccagcggg
      781 tcgtagaggt agcggctctc gaccagtggc tcgccatgct gtatccgcgt gtagaacacc
      841 aggcggtgct ggctgtcgta gtggtagtgg tgggtccgct cgtcgtgcat ccgtatcacc
      901 ccttccggga tgcggtccgt cttctccgcc agcctgccgt attcatcgtg gcggtagaca
      961 tagtgcgcat cttccgcgat gcggttatcc ggccatgccg tgagggtgct gtccgggtgc
     1021 agttccgggt ccggcagccg gtttcctgcc gggtccgtcg catacgggat atcgatatcc
     1081 aggttcgccg ccgtggtgtg gacgcccgtc agccttcccg tgtcgctgta acggtactcc
     1141 cggctctcct gcgggccgct gatgcggatt agttgtccgt tatcgttcca cgtatagtca
     1201 cagtcaagct gcggaaggtt caggtgcctg ctctgtagct ggccgctggt attccaggcg
     1261 gtggcaagtt cacaggcctc cccgccgaag ctgcgcaccg tctcccggtg cagccggtcc
     1321 cgcgtgtact cgaccagtgg cgttccgccc agcttcatcc ccgcaagata accgctgcca
     1381 tacgtcagcc actccaccgg cggcaggccg tccggctcct gacgggtcgc cagcccctgt
     1441 tcgctgtatg catgccccgt ctcatgctcc cacagtatct cccccgtctc cgggttctcc
     1501 accgtctgcc gttcgcccgt caggcggcct ttatcgtcat agccgtagtg gaccgccacg
     1561 cggtggcctt cactggtatg gctgagggtg gttaaccacc cgtgctcatc atactgccac
     1621 tgctccgccg ggtcgccgtt caccgtccgg tgcgtgatgc ggtccgacgc atcgtagtgc
     1681 cagagggtga taagtccctc gtcttcactc tgcgtgagtt ttccggtcag gtcatactgg
     1741 taacgttgcg tcctgccatc aaagccgcgc tgctccgtca gcctgtccac ggggtcatac
     1801 aggaacgtgg actggctgcc gttctcatta gtcagcgtgg tgatgcgccc ggcggcgtca
     1861 taccccatgc tgcgcgtcag tccgccttcc gtcacactca ccggacgccc gcgtttatca
     1921 tattcgatgg tgctgcgttt accgtccggt gatatcgtcg ctgtcaggtc gcctgcggcg
     1981 ctgtactcat aacgggtttc acgcccctgc gcatccttct gactgaccag ttggccacgc
     2041 ggattataac tgctgtaagt gctgatgcct tcttcccggt gaatggcgat ttgttgaccg
     2101 taccggtcat attcataccg cgtcgcgtac cccgagcagt ccgtaaaggc ctgaagctga
     2161 ccgtaccggt ttcgcgttat tctccgcctc accccggttc cgtcttccac cgcacagggc
     2221 aggccactgc gggaagaatc ataaaaccag cgcgtaatgt taccgttgcg cgagatctct
     2281 tctgccagtc ggccctgacg gtcatatttc cggctgctgc gtaaaccatc cggatatgtc
     2341 actgaagtca gttgca
//
LOCUS       DAZFFI010000276         2337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFFI010000276 DAZFFI010000000
VERSION     DAZFFI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.278"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..>2337
                     /locus_tag="ACHJ12_005146"
     CDS             26..>2337
                     /locus_tag="ACHJ12_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4992771.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHAYLAANSLQGPWWILSWPERVPEVDEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLALTTQAQRAESFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     AYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPET"
BASE COUNT          483 a          641 c          833 g          380 t
ORIGIN      
        1 aaattcagag gaagaggaat aaaccatgag cggaaaaccg gcagcgcggc agggtgacat
       61 gacccgcaag ggactggata ttgtgcaggg ttcagcgggg gtgctgatag gtgcgccgac
      121 gggcgtggcc tgctcggtat gtccgggagg gattacctat gctaacccgg tgaacccggt
      181 gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg ctgcccggcc cgctgccgtt
      241 tattctttcc cgcgcttaca gcagctaccg gaccaggacg cccgcgccgg tgggggtgtt
      301 cggtcccggc tggaaagcgc ccttcgatat tcgcttacag atacgcgatg aaggcctgat
      361 actcaacgac aacggcggca ggagcattca ctttgagccg ctgtttcccg gcgagataag
      421 ctacagccgc agcgagtcgt tctggctggc gcggggcggg gtggcggcgc agcacagctc
      481 gcagccgtta agcgcgctct ggcaggtgct gccggaagat gttcgcctga gtccgcatgc
      541 gtatctggca gcaaacagcc ttcaggggcc gtggtggata ctcagctggc cggagcgggt
      601 gccggaggtg gacgaggtgc tgccgccgga gccgcccgca taccgggtgc tgacgggcgt
      661 ggtggacggc ttcgggcgga cgctggcctt tcaccgggcg gcggagggtg atgtggcggg
      721 tgcagtgacg ggggtgacgg acggcgcagg ccgccgtttt cacctggcgc tgaccacaca
      781 ggcgcagcgg gcggaatcct tccgtaaaca gcgcgccacg tctttatctt cccctgccgg
      841 tccccgttct gcttcctctt cttctgcttt ccccgacacg ctgcccgccg gtacagaata
      901 cggtgccgat aacggtatcc ggctggaagc ggtgtggctg acacacgacc cggcataccc
      961 ggatgaacag ccgactgcgc cgctggcgcg ctacacgtac acggccagcg gagaactgcg
     1021 ggcggtgtat gaccgcagcg ggacgcaggt tcgcgggttt acttatgatg cggagcacgc
     1081 cgggcggatg gtggcacacc attatgcggg gaggccggag agccgctacc ggtatgatga
     1141 taccggtcgg gtgacggagc aggtcaaccc ggaggggctg gactatcgct ttgagtacgg
     1201 gcaggaccgt gtgaccatca cggacagcct gaaccggcgg gaggtgctgt acacggaggg
     1261 cgaaggcggc ctgaagcgtg tggtgaagaa ggagcatgcg gacggcagca tcacccgcag
     1321 cgagtatgat gaggcgggga ggctgaaggc gcagacggat gcggcgggac ggcggacgga
     1381 gtaccgactg catatggcgt ctggaaagct gacttcagtg atactgccgg atggcaggac
     1441 ggtacggtat ggctataaca gccagcggca ggtgacgtcg gtgacgtacc cggacgggct
     1501 tcgcagcagc cgggagtatg atgagaaggg aaggctggcg gcggagacct cacgcagcgg
     1561 tgagacgacg agttacagct atgatgaccc ggcgagtgag ctgccgacag ggatacagga
     1621 cgcgacgggc agtacaaaac agatggcatg gagccgttac ggtcaactgc tggcctttac
     1681 ggactgctcg gggtacacga cgcggtatga gtacgaccgg tacggtcaac aaatcgccgt
     1741 tcaccgggaa gaaggcatca gcacttacag cagttataac ccgcgtgggc aaatggtcag
     1801 tcagaaggat gcgcaggggc gtgaaacccg ttatgagtac agcgccgcag gagacctgac
     1861 agcaacggta tcaccggacg gtaaacgcag caccatcgca tatgataaac gcgggcgtcc
     1921 ggtgagtgtg acggaaggcg gtctgacgcg cagcatgggg tatgacgctg ccgggcgcat
     1981 caccgtgctg accaacgaga acggcagcca gtccacgttc cggtatgacc cggttgacag
     2041 gctgactgaa cagcgcggtt ttgacggccg gacgcaacgt tatcattatg acctgaccgg
     2101 aaaactcacg cagagtgaag atgaggggct tgtcaccctc tggtactacg atgaatcgga
     2161 ccgtatcact caccgcacgg tgaacggcga cccggcagag cagtggcagt atgatgagca
     2221 cgggtggtta accaccctca gccataccag tgaaggccac cgcgtgtcgg tccactacgg
     2281 ctacgacgat aagggccgcc tgacgggcga acggcagacg gtggagaacc cggagac
//
LOCUS       DAZFFI010000277         2302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFFI010000277 DAZFFI010000000
VERSION     DAZFFI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.279"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            259..684
                     /locus_tag="ACHJ12_005147"
     CDS             259..684
                     /locus_tag="ACHJ12_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992772.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            681..1031
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005148"
     CDS             681..1031
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4992773.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            1062..>2302
                     /locus_tag="ACHJ12_005149"
     CDS             1062..>2302
                     /locus_tag="ACHJ12_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO4992774.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTS"
BASE COUNT          597 a          575 c          645 g          485 t
ORIGIN      
        1 tggtttattg cccggggatt cagggctgaa aggatacatg caacgggccg tggatatgat
       61 gcaccagtgg tgttctgccc ggatgtggag ggcgaagcac tgaaggaatg tctgcgcccc
      121 aaccgcaggg ttgaaataac cggggaaaca tcagtgactg attaacaata aaggtaagcg
      181 taaactgacc gccgtatgta gccatcagac gagaattggt aacttagacg cccatctgat
      241 atagacggac atctaagtat ggaattacag gactggcgaa aagaacctcg taaaaactat
      301 tcgaatgaat tcaaacttcg tatggtggaa ctggcatcac aacctggagc ttgtgttgca
      361 cagattgcac gtgaaaatgg cgtcaatgat aatgttattt tcaaatggct caggctctgg
      421 cagaacgaag ggcgtgtttc gcggcgtctt ccggtaacga cctcttctga cactggcgtt
      481 gaattattac ctgtagaaat aacgccggat gagcagaaag aacctgtggc ggccattgcg
      541 ccgtctttat ccacttccac tcagaccaga gtcagtgcca gttcctgcaa ggtggaattc
      601 cgtcacggta acatgacgct ggaaaatcca tcgccagagc tgctcacggt gttgatccgt
      661 gaactgaccg ggaggggaag atgatctcac tcccatcagg tacccgtatc tggctcgttg
      721 ccggcgttac cgatatgcgt aaatccttca acggactggg agaacaggta caacatgtgc
      781 tgaatgataa tcccttctcc ggtcacctgt ttatcttccg tggccgacgg ggtgacaccg
      841 tcaaaattct ttgggctgat gctgatggtc tgtgcctgtt caccaaacgc ctggaggaag
      901 gccagtttat ctggcctgcg gtacgtgacg gcaaggtatc cattacccgc tcgcaactgg
      961 caatgctcct cgataagctg gactggcgtc agccaaaaac atccagccgt aactcactga
     1021 caatgttgta aaaaactcct gaccgcatta taaaaacggt catgagtcag aaatacctca
     1081 ttcgcatcgc agagctggaa agattgctct ctgagcaggc tgaagccctc cgtcagaaag
     1141 accagcaact gagtctggtt gaagagacgg aagccttcct gcgctctgca ctgacacgtg
     1201 ccgaagaaaa gatcgaagaa gatgaacggg aaatagaaca tctgcgggct cagatagaaa
     1261 aactgcgccg gatgctgttc ggtacccgtt ctgaaaaact gcgtcgtgaa gttgaactgg
     1321 ctgaggctct gctgaaacaa cgtgaacagg acagcgatcg ttacagtggg cgggaagacg
     1381 atcctcaggt tccccgccag ttgcgacagt cgcgccatcg tcgtccgtta ccggcacacc
     1441 ttccccgtga aatacaccgc ctggagccag aagaaagctg ttgcccggag tgtggcggtg
     1501 aactggatta tctgggggaa gtcagcgctg aacagctgga actggtgagc agtgccctga
     1561 aagtgatccg cacagaacgg gtaaaaaaag cctgtacaaa atgtgactgt attgttgaag
     1621 caccggcgcc gtcccgcccg atagagcgtg gtatcgcggg ccccggatta cttgcccgcg
     1681 tgttaacggg aaaatactgc gaacatctgc cactgtatcg tcagagtgaa atctttgccc
     1741 gccagggtgt cgaactgagc cgggccttac tctccaactg ggttgacgcg tgctgccagt
     1801 taatgacacc ggtgaatgat gccctgtacc gttatgtaat gaacacccgc aaggttcaca
     1861 ctgatgacac accggtaaag gtactggcac cgggtcagaa aaaggcgaaa acagggcgta
     1921 tctggacgta tgtccgggat gatcgcaatg tgggttcgtc atctcctcca gcggtctggt
     1981 tcgcgtactc gccgaaccgg caggggaaac acccggagca acacctccgc cccttccggg
     2041 gtatcctgca ggcggatgcg ttcacaggtt acgacaggtt gttcagtgca gaacgtgaag
     2101 gtggtgcact gacagaagtt gcgtgctggg cccatgcccg gcgaaaaatc cacgatgtat
     2161 acatcagcag caaaagtgcg acggcagaag aagccctgaa gcgaatcagt gaactgtacg
     2221 ccatcgagga tgaaatacgg ggattaccgg agtcagagcg tcttgccgtc aggcagcagc
     2281 gaagcaaagt gttactgacg tc
//
LOCUS       DAZFFI010000278         2296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFFI010000278 DAZFFI010000000
VERSION     DAZFFI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.280"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2296
                     /locus_tag="ACHJ12_005150"
     CDS             <1..>2296
                     /locus_tag="ACHJ12_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992775.1"
                     /translation="DDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTT
                     LSHTCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEP
                     DGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYT
                     LTGQLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLD
                     IDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPT
                     GVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDL
                     TGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKAR
                     HRSLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQL
                     EAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRL
                     PGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKF
                     HVNGDPSDFNQAVEYLKQDSQMKET"
BASE COUNT          546 a          612 c          783 g          355 t
ORIGIN      
        1 tgatgacccg gcgagtgagc tgccgacagg gatacaggac gcgacgggca gtacaaaaca
       61 gatggcatgg agccgttacg gtcaactgct ggccttcacg gactgctcgg ggtacacgac
      121 gcggtatgag tacgaccggt acggtcaaca aatcgccgtt caccgggagg aaggcatcag
      181 cacttacagc agttataacc cgcgtgggca actggtcagt cagaaggatg cgcaggggcg
      241 tgaaatccgt tatgagtaca gcgccgcagg cgacctgaca gcaacggtat caccggacgg
      301 taaacgcagc accatcgaat atgataaacg cgggcgtccg gtgagtgtga cggaaggcgg
      361 tcttacgcgc agcatggggt atgacgctgc cgggcgcatc accgtgctga ccaacgagaa
      421 cggcagccag tccacgttcc ggtatgaccc ggtggacagg ctgactgaac agcgcggttt
      481 tgacggccgg acgcaacgtt accactatga cctgaccgga aaactcacgc agagtgaaga
      541 cgaggggctt atcaccctct ggcactacga tgcgtcggac cgcatcacgc accggacggt
      601 gaacggcgac ccggcagagc agtggcagta tgatgagcac gggtggttaa ccaccctcag
      661 ccatacctgt gaaggccacc gcgtgtcggt ccactacggc tatgacgata aaggccgcct
      721 gacgggtgaa cggcagacgg tggagaaccc ggagacaggg gagatgctgt gggagcatga
      781 gacggggcat gcgtacagcg aacaggggct ggcgacccgt caggagccgg acggtctgcc
      841 gccggtggag tggctgacgt atggcagcgg ttatcttgcg gggatgaagc tgggcggaac
      901 gccgctggtc gagtacacgc gggaccggct gcaccgcgag acggcccgta gcttcggcgg
      961 ggcgggcagt acagcggggt acgaacaggc cacggcgtac accctcacag ggcagctaca
     1021 gagcaggcac ctgaaccttc cgcagcttga ctgtgactat acgtggaacg ataacggaca
     1081 actggtgcgc atcagcggcc cgcaggagtg ccgggagtac cgttacagcg gcacgggaag
     1141 gctgacgggc gtccacacta cggcagcgaa cctggatatc gatatcccgt atgcgacgga
     1201 cccggcagga aaccggctgc cggacccgga actgcacccg gacagcaccc tcacggcgtg
     1261 gccggataac cgcatcgcgg aagatgcgca ctatgtctac cgctacgatg aatacggcag
     1321 gctggcggag aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac
     1381 ccaccactac cactacgaca gccagcaccg cctggtgttc cacacgcgga tacagcacgg
     1441 tgagccgcag gtggagagcc gctacctcta tgacccgctg ggccgccgga cgggaaaacg
     1501 ggtgtggcgg cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggagga
     1561 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
     1621 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
     1681 tgaacaggcg aaggcgcggc accgtagcct ggcggaggtg ttgcaggagg acacgggtgt
     1741 gacgctaccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcggcaggg
     1801 cagcgtgagt gaagaaagcc agcagtggct tgcgcagtgc gggctgacgg cggaacagat
     1861 ggccgcgcaa ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgtga
     1921 ccaccgtggg ctgccgctgg cgcttatcag cccggaaggg gaaacggcgt ggcaggggga
     1981 gtatgacgag tggggaaacc tgctgggcga ggaaagcgcg cagcaccttc agcagtcact
     2041 ccgtctgccg ggacagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
     2101 ctatgatccg ttgcagggga gatatatcac ccaggacccg atagggctga gaggtgaatg
     2161 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
     2221 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
     2281 acaaatgaaa gaaact
//
LOCUS       DAZFFI010000279         2287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFFI010000279 DAZFFI010000000
VERSION     DAZFFI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.281"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..303
                     /locus_tag="ACHJ12_005151"
     CDS             154..303
                     /locus_tag="ACHJ12_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992776.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            587..847
                     /locus_tag="ACHJ12_005152"
     CDS             587..847
                     /locus_tag="ACHJ12_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4992777.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1072..1146
                     /gene="tap"
                     /locus_tag="ACHJ12_005153"
     CDS             1072..1146
                     /gene="tap"
                     /locus_tag="ACHJ12_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4992778.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1139..1996
                     /gene="repA"
                     /locus_tag="ACHJ12_005154"
     CDS             1139..1996
                     /gene="repA"
                     /locus_tag="ACHJ12_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4992779.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT          584 a          572 c          602 g          529 t
ORIGIN      
        1 atcgcggcgg gtgtctgagc ctttctggtt caggcaagac gcaggtacca gaaatgcgaa
       61 gacccacttg ttaatccatt aactcgtgag gcctgcatga agtacttgaa cactactgat
      121 tgtagcctct tccttgcaga gaggtcaaag tttatgacga aatatgccct tatcgggttg
      181 ctcgccgtgt gcgccacggt gttgtgtttt tcactgatat tcagggaacg gttatgtgag
      241 ctgaatattc acaggggaaa tacagtggtg caggtaactc tggcctacga agcacggaag
      301 taagctgccg ggcggggacg gaagtccccg ctttccggaa gtgtgaggta tttcaggggc
      361 agacacccga catgccagaa accgccggtc ccgcccgggg ccggcacaca ggttaaggca
      421 tttcctgttt ttcagccatt tcgcaacaaa aatcacatta aacaacagta atcaccgttg
      481 atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt
      541 agtatcctct gcgaaacgat ccctgtttga gaattgagga ggcgagatgt cgcagacaga
      601 aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac
      661 tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa
      721 agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat
      781 aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggatatact
      841 aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt
      901 gtggctggcc acgccataag gtggcaggga actggttctg atgtggatgt acaggagcca
      961 gaaaagtgaa aaccccgata atcttcttta actttggcga gtgagaaaga ttaccggggc
     1021 catctaaaaa ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga
     1081 aaagttcaag acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt
     1141 gactgatctt caacaaacgt attaccgcca ggtaaagaac ccgaatccgg tgtttacacc
     1201 ccgtaaaggt gccggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt
     1261 cacctcccgt tttgatttcg ccattcatgt ggcgcatgcc cgttcgcgtg gtctgcgtcg
     1321 acgcatgcca ccggtgctgc gtcgacgggc tattgatgcg ctgctgcagg ggctgtgttt
     1381 ccattatgac ccgctggcca accgcgtcca gtgttccatt accacactgg ccattgagtg
     1441 cggactggcg acagagtccg gtgcaggaaa actctccatc acccgggcca cccgggccct
     1501 gacgttcctg tcagagctgg gactgattac ctaccagacg gaatatgatc cgcttatcgg
     1561 gtgctacatt ccgaccgata tcacgttcac accggcgctg tttgccgccc ttgatgtgtc
     1621 tgaggatgca gtggcagctg cgcgccgcag ccgtgtggta tgggaaaaca gacagcgcaa
     1681 aaagcagggg ctggataccc tgggtatgga tgagctgata gcgaaagcct ggcgttttgt
     1741 ccgtgagcgt ttccgcagtt accagacaga gcttaagtcc cacggcatga aacgtgcccg
     1801 tgcgcgccgc gatgcaggca gaacccgcca ggacattgtc accctggtga aacggcagct
     1861 gacgcgtgaa atcgcggaag ggcgtttccg gggaagcctg gaggccgtga agcgcgaaat
     1921 cgaccgtcgt gtgaaggaac gcatgataat gtcgcggaac aacaactaca cacggctggc
     1981 cactgcctcc ccctgagttt tacctcatct gaataatccg gcctgcgccg gaggcatctg
     2041 cacgcctgaa gcctgtcagc gaacaaaaaa acagcaccac atacaaaaaa caatctcatc
     2101 atcccgcttc tggtgcatcc ggcccccctg ttttcgatac aaaacatgcc tcacagacgt
     2161 ggaattttgc ttatccacat ttaactgcaa gggacttccc cataaggtta caaccgttca
     2221 tgtcataaag cgccagccgt cagcgttaca gggtgcaatg tatcttttaa acacctgttt
     2281 atatctc
//
LOCUS       DAZFFI010000280         2209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFFI010000280 DAZFFI010000000
VERSION     DAZFFI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.282"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHJ12_005155"
     CDS             <1..315
                     /locus_tag="ACHJ12_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO4992780.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGESARQVVPGLMIRE
                     AS"
     gene            312..920
                     /locus_tag="ACHJ12_005156"
     CDS             312..920
                     /locus_tag="ACHJ12_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO4992781.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            935..1084
                     /locus_tag="ACHJ12_005157"
     CDS             935..1084
                     /locus_tag="ACHJ12_005157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992782.1"
                     /translation="MAEINFQVLREAEQVKRENQPVEAGAWKDAVEWVLQEDCQAVNI
                     RIKGE"
     gene            1087..1374
                     /locus_tag="ACHJ12_005158"
     CDS             1087..1374
                     /locus_tag="ACHJ12_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992783.1"
                     /translation="MATLQELIDLTPEQEKAWKRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTARVYMPGIDAPGLTSWADDWHGITLKDGVEVDDD"
     gene            1378..1776
                     /locus_tag="ACHJ12_005159"
     CDS             1378..1776
                     /locus_tag="ACHJ12_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992784.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGGMCVDGVGEYVGHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVDWVLKEACQAVNIRIKGE"
     gene            1779..>2209
                     /locus_tag="ACHJ12_005160"
     CDS             1779..>2209
                     /locus_tag="ACHJ12_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO4992785.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWVESSVALKSTRDPVGSGGVP"
BASE COUNT          533 a          482 c          721 g          473 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggagcag caggatatgg tgacggtgga ctcccgtgag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt gggggagagt gcccgacagg tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccggctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgcattg accgggcact ggcgctggga ggctccattg
      601 aactgatggc gttttacttt ggactttcca gtgtggatgt ggcagcgcgc cgtcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag agcggcatca cagatgtgga aagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgcagtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agcagaatga caaggacaaa tgacatggcc gaaattaact ttcaggtact
      961 gcgggaggct gaacaggtga agcgagagaa ccagccggtt gaggctggag cgtggaaaga
     1021 tgccgttgag tgggtgctcc aggaggattg ccaggctgta aacattcgca tcaaaggaga
     1081 gtgaacatgg ctactttgca ggaattaatc gacctgacgc cagaacagga aaaagcgtgg
     1141 aagcgccttg tgaaggctgt aaaggatttc agggcagccg gaggaaagtt ttatagcgtc
     1201 ctggacacgc tgagtgcata caacggcgag cacgttgcca gcattgataa cgataagggc
     1261 taccacactg caagagtcta tatgcctggc attgatgcgc cagggctaac cagttgggct
     1321 gatgactggc acggcatcac gctgaaagat ggcgttgaag tggatgatga ctaacacatg
     1381 accacgatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgacatt
     1441 gacgatatag acggcggtga gattcgtgag ctggcgctta tcgcgctggc atcgctggaa
     1501 gcagagcctg tggcgtcatg cattattgaa gatgggggca tgtgtgttga cggggtcggt
     1561 gagtatgtag gtcactcgct gcctggtgga acgtaccagc tatacaccgc ttcacctgtg
     1621 ccggtagtgc ctgaaggact ggttaaagca gtgcgctttt atgaacaggt aaagcgtgag
     1681 aatccgccag ttgaaactgg ggcatggaaa gatgcggttg actgggtgct caaagaggct
     1741 tgccaggctg taaatattcg catcaaagga gagtgagcgt gcagatttca ccagtcactc
     1801 ttcgtgttgc gaaggcgttt atatccagac atcaccgaca caataaacct cctgtggggc
     1861 ataaattcag tattggtctg agaaatgatg cgggtgaatt gacaggtgtg gcgacagccg
     1921 gcagacctgt tgcacgccac tttgacgatg ggctgacact ggaggtcaat cgtacctgta
     1981 ctactggtga acgtaacgct aacagcgcac tttatggcgc tgtctggagg gcggcaaaag
     2041 atatgggcta tcgccgttgc attacataca cccgggctga tgaatccggg gcctctcttc
     2101 gggcagctgg ttttgttcgt gtgaaagaac ttccttcagg aaaaagctgg gtggaatcaa
     2161 gtgtcgccct gaagagtaca cgcgatccgg ttggaagcgg tggtgttcc
//
LOCUS       DAZFFI010000281         2200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFFI010000281 DAZFFI010000000
VERSION     DAZFFI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.283"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..278)
                     /locus_tag="ACHJ12_005161"
     CDS             complement(<1..278)
                     /locus_tag="ACHJ12_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4992786.1"
                     /translation="MATTNAFCLASPPLVRICLHGDLQRFGRRICLYVNTAAEAIRAL
                     SLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYARLHEPLSGRAVIHIVPR"
     gene            complement(176..919)
                     /locus_tag="ACHJ12_005162"
     CDS             complement(176..919)
                     /locus_tag="ACHJ12_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4992787.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCVLAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            complement(930..1628)
                     /locus_tag="ACHJ12_005163"
     CDS             complement(930..1628)
                     /locus_tag="ACHJ12_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4992788.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(1628..1969)
                     /locus_tag="ACHJ12_005164"
     CDS             complement(1628..1969)
                     /locus_tag="ACHJ12_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992789.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVMTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(1962..>2200)
                     /locus_tag="ACHJ12_005165"
     CDS             complement(1962..>2200)
                     /locus_tag="ACHJ12_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4992790.1"
                     /translation="AIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANG
                     QIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          482 a          755 c          531 g          432 t
ORIGIN      
        1 cgcggtacaa tatgaatcac ggccctcccg ctcagtggct catgaagacg ggcatacacc
       61 gcctccggcg cggtatcctc accggcaata cgtatctgat accagccttc gttcatctga
      121 ccgcggaatc ccggcacctg cagcgacagg gcacggatgg cctccgctgc cgtgttcaca
      181 tacaggcaga tgcggcgacc aaatcgttgt aaatccccgt gaaggcagat gcggaccagt
      241 ggcggtgacg ccagacagaa tgcgttcgtc gttgccattt ttcagaatac ctctcccgtt
      301 tactcagttg ttcaggtata tggtgaagca gttcaccgtt gccacagtaa atggcggcat
      361 gattcggcac cgatgaacca aagcagcaca gcaggatatc gcctgccagt acacaggacg
      421 gagacacccg gtaaaagccg ttttccgcca ggttgtccag gtacaggttc tggccgttgc
      481 gccaccagtc atcctcacgc acaaaatccg gcagcgttat ccccgccaga tggtatgcat
      541 cccggaacag ggtgtaacag tccgtcacac catgttcaaa gcgccgtccg gtcaggtgcg
      601 gcacacagcg gaaccggtga atgtcacccc ggcagaccag ccaccagggc agggcactct
      661 ttatctgcag cctccggtcc gcctcgctca gccagggcag accaccggga tgactgtgga
      721 ccagcgccac aatctccccc tgcatctctg cccgcagcca gtcttccggt gcgatacgaa
      781 aatacgcctc cggctctgca gaaatattca cacaagggat ataccactcc ccctccgggg
      841 tgcttatcac gaagccgcac gactccgcag gcgcacaccg ccgggcatgc accagaatcg
      901 ctgattcagt ctgtgtcata aaccgggatt tactgcgaaa gtttattaat ggaaaggaaa
      961 ccgccaaaat tgccgacatt cctgcgcagt tcacacccgc gcatgcactt gctgcatctg
     1021 tccttacgga tatccgtggt gggtttatcg aactcatccg ccacagcccc gcccgtgtaa
     1081 ccacactcat cagagcggta ggtccacata caggtgttcg ccagcatgat acgaccggga
     1141 aacagcgccc cgtccgtctc ggtcggtgtc gccagcacaa acgaggccgt catggccgtc
     1201 agcgctgaca tctgctccac cacccaccgg tccgtcagct cctgctccgg gtctgcctca
     1261 ggattgcctg ccacaaaatt caccgcatcc agaaaacgcg catacacccg gcgacggacc
     1321 accgtggccc ccaccaggct ctgcaaatcc tccgccatcc cggtgacaag gccaaacaga
     1381 ttggacaccg tcagcgacgg gcgggcactg ctgccctttc cgttcatctc aaagccgctg
     1441 ccctcaatcg ggtacgcctg atattcacgc ccctgccagg tcaccggctc ccctttttca
     1501 ttcagctcat tgcagaaaaa ataccgctca ccgccctgca ccgtcaggtc gatttcccag
     1561 agcaccaccc gcggtgactg ctctgactta accgactcgt tcaggctttc ttcgtgaata
     1621 tcctgcatca gttcaccacc tgctctatcg tgcaactgaa atcactgtac cgggcgttat
     1681 ccgtaatact ccactcccgg cacaccaccc tgaccgtccg gttatgtttc gggggcttcc
     1741 acaaaaaagc ccggtaacca ccatgccatg acagaaacgc ggacagtgcc tcccgctcag
     1801 catccgttgt cacccgaaaa accacctgaa aggtcttcag ttgcgcattc agtcctgccg
     1861 gacgacgctg ctgataaccg tcgccaaact tcaccgtcat caccgacggt ttctccgtca
     1921 cctgcatccc ttcccgggga caccagtgaa gagtcttaat ctcatccact cagcattcct
     1981 ccgtcacgac gcatggataa catcaccgcc tgtacccgct ggtcaatcag ttgcacaagg
     2041 gtgcccgccg cttccggccc tatctgcccg ttagcgccgt cattctgaat ggcgatgtgg
     2101 tacaccgggg aatacaccag acccgcactg ccgttcatac tgcccaccgc acgcacgccg
     2161 agtgaaccat ccgctgcccg ggtcaggggc atgatggctt
//
LOCUS       DAZFFI010000282         2199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFFI010000282 DAZFFI010000000
VERSION     DAZFFI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.284"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..273
                     /locus_tag="ACHJ12_005166"
     CDS             <1..273
                     /locus_tag="ACHJ12_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4992791.1"
                     /translation="YSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNPDEVK
                     RRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            263..>2199
                     /locus_tag="ACHJ12_005167"
     CDS             263..>2199
                     /locus_tag="ACHJ12_005167"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4992792.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVA"
BASE COUNT          538 a          531 c          664 g          466 t
ORIGIN      
        1 tattccggtc cggtgatgcc gtggatcgac ccggttaaag aagctgaagc ctggagaatt
       61 cagatccggg gtggtgcagc gacagaatct gactgggtgc gtgccggcgg gcgcaatccg
      121 gatgaggtca aacgtcgccg caaggctgaa attgatgaaa acagcagact ggggctggtc
      181 tttgatactg accccgtcaa cgacaaagga ggcaacagtg ccggaactga acgacagtat
      241 cagcgtgaca ccgaaagcca gcatgaagaa taaatcctgg ttcaggatgc aggctggggg
      301 gccgggtgac gcggatattt atatttatga cgagattggt ttctggggag ttaccgcgaa
      361 gcagtttgtc agcgaactga atgcactggg tgatatcacc cacattaatc tccatatcaa
      421 ttcaccgggt ggcgatgtct ttgaaggcat cgccattttt aatgccctga aaaatcaggg
      481 ggcgaccatt accgtgtatg tggatggcgt tgccgcctcg atggcatctg tgattgcgat
      541 ggccggtgat acggtcatta tgccggaaaa tgccttcatg atgatccata agccatgggg
      601 attcagtggc ggggatgctg aggatatgcg cagttatgct gatttgctgg ataaagtcga
      661 atcggtactg ttgccagcct atgcgcagaa aaccggaaaa accaccgatg aaattgccgc
      721 catgctggcg gatgaaacct ggatgtccgg tgccgaatgt ctggcacacg gatttgctga
      781 ccaggtgaca cccgctgttg aggcaatggc atgtattcag tcaaaacgta cagaggaatt
      841 taaaaagatg ccggaatcca tccgaaacat gattactcca ccacgcaaca gtgccccgcg
      901 tgataccaca gtgacaatcc ctgcaccggc ggtaacagaa ccatcaccgg taccggcagt
      961 gtctgatgag gcgaccattc gcgcccgcgt tatggctgag cagaaagcac gcatgtcagg
     1021 cattaacgat ctgtttgcca tgttcggcgg tcgctatcag acgcttcagg cacagtgcgt
     1081 ggctgatcct gactgttctc tggaaatggc ccgtgaacgt ctgctgaatg aaatgggcaa
     1141 ggagtcctcg ccgaccaaca aaaatacacc ggcccatatt tatgccggaa acggcaattt
     1201 tgtgggggac gggatccgcc aggcgatgct ggcccgtgcc ggatttgaaa atgtcgagaa
     1261 ggataacgcc tataacggga tgaccctgcg tgaatgggct cgcatgtcac tgacggagcg
     1321 cggtattggg gtggccagtt ataaccccat gcagatggtc gggctggcgc tgacgcacag
     1381 cacctctgat tttggcaata ttctgctgga tgtgtcgaac aaggggctga tccagggctg
     1441 ggaggaatca gaagaaacct tccagaagtg gacccgtaag ggacgcctgt cagacttcaa
     1501 aacagcgtat cgcgtgggga tgggcggttt tggttctctg cgtcaggttc gtgagggggc
     1561 ggagtataaa tacatcacca cctcagatcg caaggagacc attgcactgg ccacttacgg
     1621 ggagattttc tccatcaccc gccaggccat tatcaatgat gatctgaata tgctggtgga
     1681 cgtgccgatg aagatggggc gtgcggcgaa ggcaacgatt ggtgacctgg tctacaaggt
     1741 gctgacggat aacccgaaac tgtccgacgg taaggcgctg ttccatgccg atcacaaaaa
     1801 tattgccacc ggggggatct ccgtttccgg actggatgcg gcccgtcaga tgatgcgcct
     1861 gcagaaagaa ggcgatcgtg ccctgaatat ccgtccggcc tttatgctgg taccggtggc
     1921 actggagacg gtggcgaacc agaccatcaa atcggccagt gtgaaagggg cggatgcaaa
     1981 cgccggtgtc attaacccta tccagaactt tgctgaggtg attgcagaag cgcgtcttga
     2041 tgcggcagac ccgaaaacct ggtatctggc ggcggcacag ggcactgaca ccattgaagt
     2101 ggcctggctg gatggtgtgg acacgccata cattgatcag caggaaggtt tcaccactga
     2161 cggtattgcc acaaaaatcc gtattgatgc cggagtggc
//
LOCUS       DAZFFI010000283         2199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFFI010000283 DAZFFI010000000
VERSION     DAZFFI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.285"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..273
                     /locus_tag="ACHJ12_005168"
     CDS             <1..273
                     /locus_tag="ACHJ12_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4992793.1"
                     /translation="YSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNPDEVK
                     RRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            218..>2199
                     /locus_tag="ACHJ12_005169"
     CDS             218..>2199
                     /locus_tag="ACHJ12_005169"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4992794.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQTGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITNINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKECSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVA"
BASE COUNT          538 a          530 c          666 g          465 t
ORIGIN      
        1 tattccggtc cggtgatgcc gtggatcgac ccggttaagg aagctgaagc ctggagaatt
       61 cagatccggg gtggtgcagc gacagaatct gactgggtgc gtgccggtgg gcgcaatccg
      121 gatgaggtca aacgtcgccg caaggctgaa attgatgaaa acagcagact ggggctggtc
      181 tttgatactg accccgtcaa cgacaaagga ggcaacagtg ccggaactga acgacagtat
      241 cagcgcgaca ccgaaagcca gcatgaagaa taaatcctgg ttcaggatgc agactggggg
      301 gccgggtgac gcggatattt atatttatga cgagattggt ttctggggag ttaccgcgaa
      361 gcagtttgtc agcgaactga atgcactggg tgatatcacc aacattaatc tccatatcaa
      421 ttcaccgggt ggcgatgtct ttgaaggcat cgccattttt aatgccctga aaaatcaggg
      481 ggcgaccatt accgtgtatg tggatggcgt tgccgcctcg atggcatctg tgattgcgat
      541 ggccggtgat acggtcatta tgccggaaaa tgccttcatg atgatccata agccatgggg
      601 attcagtggc ggggatgctg aggatatgcg cagttatgcc gatttgctgg ataaagtcga
      661 atcggtactg ttgccagcct atgcgcagaa aaccggaaaa accaccgatg aaattgccgc
      721 catgctggcg gatgaaacct ggatgtccgg tgccgaatgt ctggcacacg gatttgctga
      781 ccaggtgaca cccgctgttg aggcaatggc atgtattcag tcaaaacgta cagaggaatt
      841 taaaaagatg ccggaatcca tccgaaacat gattactccg ccacgcaaca gtgccccgcg
      901 tgataccaca gtgacaatcc ctgcaccggc ggtaacagaa ccatcaccgg taccggcagt
      961 gtctgatgag gcgaccattc gcgcccgcgt tatggctgag cagaaagcac gcatgtcagg
     1021 cattaacgat ctgtttgcca tgttcggcgg tcgctatcag acgcttcagg cacagtgcgt
     1081 ggctgatcct gactgttcgc tggaaatggc ccgtgaacgt ctgctgaatg aaatgggcaa
     1141 ggagtgctcg ccgaccaaca aaaacacacc ggcccatatt tatgccggaa acggcaattt
     1201 tgtgggagac gggatccgcc aggcgatgct ggcccgtgcc ggatttgaaa atgtcgagaa
     1261 ggataacgcc tataacggga tgaccctgcg tgaatgggct cgtatgtcac tgacggagcg
     1321 cggtattggg gtggccagtt ataaccccat gcagatggtc gggctggcgc tgacgcacag
     1381 cacctctgat tttggcaata tcctgctgga tgtgtcgaac aaggggctga tccagggctg
     1441 ggaggaatca gaagaaacct tccagaagtg gacccgtaag ggacgcctgt cagacttcaa
     1501 aacagcgtat cgcgtgggga tgggcggttt tggttctctg cgtcaggttc gtgagggggc
     1561 ggagtataaa tacattacca cctcagatcg caaggagacc attgctctgg ccacctacgg
     1621 ggagattttc tccatcaccc gccaggccat tatcaatgat gatctgaata tgctggtgga
     1681 cgtgccgatg aagatggggc gtgcggcgaa ggcaacgatt ggtgacctgg tttacaaggt
     1741 gctgacggat aacccgaaac tgtccgacgg taaggcgctg ttccatgccg atcacaaaaa
     1801 tattgccacc ggggggatct ctgtttccgg actggatgcg gcccgtcaga tgatgcgcct
     1861 gcagaaagaa ggcgatcgcg ccctgaatat ccgtccggcc tttatgctgg taccggtggc
     1921 actggagacg gtggcgaacc agaccatcaa atcggccagt gtgaaagggg cggatgcaaa
     1981 cgccggtgtc attaacccca tccagaactt tgctgaggtg attgcagaag cgcgtcttga
     2041 tgcggcagat ccgaaaacct ggtatctggc ggcggcacag ggcactgaca ccattgaagt
     2101 ggcctggctg gatggtgtgg acacgccata cattgatcag caggaaggtt tcaccactga
     2161 cggtattgcc acaaaaatcc gtattgatgc cggagtggc
//
LOCUS       DAZFFI010000284         2186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFFI010000284 DAZFFI010000000
VERSION     DAZFFI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.286"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1285
                     /locus_tag="ACHJ12_005170"
     CDS             38..1285
                     /locus_tag="ACHJ12_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO4992795.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            1571..>2186
                     /locus_tag="ACHJ12_005171"
     CDS             1571..>2186
                     /locus_tag="ACHJ12_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992796.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLVEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEDE
                     QKPVQPEGGQEAEKTEVSSTGSIEPGSGSSSDQVPEVNDQNSNGK"
BASE COUNT          627 a          413 c          648 g          498 t
ORIGIN      
        1 gagcagatat gtttaacagt ttgtggaccg cagggatatg acagaaaaaa cggaacggaa
       61 taaagcgtcg tcagaggtgg tcaggcagtc agacctggcg ctgttgacag atgtacaggc
      121 agcccttcag caggaaaagc atcatgccct tttcctgatg gtgatttttc tgctggtact
      181 ggtagtgacg tttgttatct gggcatggaa cagtcctctg gatgaagtga cccgaggtca
      241 ggggagtatt attcccggca gcagggagca ggttatacag acccttgacc cgggtatact
      301 gaaatcactt gaggtcaggg aaggcgatat tgttgagaaa ggtcaggtac tgctgacact
      361 ggatgatacc cggagctctg ccatgctgag ggaaagtgaa gcccgggtga ataacctgga
      421 ggccgtcagg gcaagactcc gtgcagaagc ttattccgaa tcactgacat ttccggataa
      481 cgttcctgag gatttgcggg agcgggaaag tacggtctat tttctccgga agacggagct
      541 ggcgcagagc attgccggtc tgaagcagag taaggcgctg ctggacaggg aaatagcaat
      601 gacgcggcca attgtgcgtg aaggggcaat gtcggaggtc gaactgttac gtatgcagcg
      661 tcagtcagcg gagctgcagt tacagatgga tgagaaacag aataaatacc tgacggaagc
      721 cggtgctgaa ctggtgaaga cagaagcaga gcttgctcag gcaaaagaga atatggctgg
      781 cagagccgac ccggttgagc gttcacgtct cagggccccc ctgagaggta tcgtaaaaaa
      841 tatacgggta aacacactgg gaggggtggt ttctgcagga caggacataa tggaaattat
      901 tccgctggaa gaccagttgc tgattgaagc ttatataaat ccacgggatg ttgcctatgt
      961 cagaacaggg atgccggcac ttgtaaaact gacggcttat gactatgcca tttatggtgg
     1021 tcttgacggc gtggtgacac tggtaagtcc ggatacttta cgtgaccaga aacggccggg
     1081 agatttaaaa ctggacccga atgaagctta ttaccgggta ctggttacca ccagtaataa
     1141 ttatctgaca gacaggaacg gaaaaagatt gccagttatt cctggcatga tagccagcgt
     1201 ggatataaaa accgggcgta aaacggtatt tcagtatctg attaaaccga taaccagaat
     1261 gaagcaggca cttcaggaaa gataataaaa aggtcttccg gggaaagtat tttaaatcaa
     1321 ttttaaacgg gcagaatgat ttcactttat tctgcccggg tcattgcgca tgcctgtatg
     1381 tgatttgggg ctgaagataa tataaatgtt atatctgtca ggggaaaata aatctgaaaa
     1441 aagcggaatg attaaggagg tccggtcaga gaaataaatt atctgtaatc tgattcgttt
     1501 ttatttaatg actcccttta gcggataaaa cataagagca tactggtttt attaacctga
     1561 ggatacttat atgaaaatga ttttcaccgg caaggtcagt ggagaaaaaa cagtactgac
     1621 agccggagcc cgtcatacag taaaggcaca ggccggggaa cagtatggac tggttgatga
     1681 ggtgacaggt ctggtcccgg atggtgtgga agcggaccgt tctggtgatg atttaattct
     1741 gcgtaaaaag gaagatgata cagaaatccg gattgaggga ttctgggaag aatgccagcc
     1801 gggtgaaaca cagtgtacag cagtatttaa tgttgtgggt gaaaatggtc aggtaactga
     1861 agctgtcctg acccaggatg gccctgtact ggagaatttc actgcagggc aaagtggcac
     1921 actggttgaa ggtatcaata acgggattat ctgggggggg attgcttttg gtgcagggct
     1981 ggcggctatt gcactggctg ccggaggcgg tggtggaagc agtcgtcata agcgtgcaac
     2041 tgaagatgag cagaagccgg ttcagcctga aggcggtcag gaggctgaaa aaacagaagt
     2101 atcctctaca ggctcaattg agccgggaag tggaagctct tccgaccagg taccggaagt
     2161 gaacgatcag aacagcaacg gcaagc
//
LOCUS       DAZFFI010000285         2176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFFI010000285 DAZFFI010000000
VERSION     DAZFFI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.287"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..478)
                     /locus_tag="ACHJ12_005172"
     CDS             complement(<1..478)
                     /locus_tag="ACHJ12_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4992797.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIF"
     gene            736..1236
                     /locus_tag="ACHJ12_005173"
                     /pseudo
     CDS             736..1236
                     /locus_tag="ACHJ12_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1238..1531
                     /locus_tag="ACHJ12_005174"
     CDS             1238..1531
                     /locus_tag="ACHJ12_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO4992798.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(1592..2176)
                     /locus_tag="ACHJ12_005175"
                     /pseudo
     CDS             complement(1592..2176)
                     /locus_tag="ACHJ12_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          730 a          400 c          331 g          715 t
ORIGIN      
        1 tgaaaatgat agcaaaaaga aaaaaaagca ttacatactt aaacagctta ctgctaaaga
       61 aatgttgttt tgtagaaata atgttatttt caataatgcc tggcaatgaa tactgccaaa
      121 cttcattttt ttcttttaca gcaaatttga gacctaaaac ttctatttgc tgattcaatg
      181 ttaacgttct atcttcaggt tcttgtgctg ttaattctct gaatgttact accagatcag
      241 ggtcggtagt aattattatc tcaaaactac ctgtaagctc gccataagga acgactattg
      301 tgttttctgg taactcagaa aaagcatcac ttcttcctaa cgaaacatca ttaccaatga
      361 taacaagtag acgcccatta agtatttcat actcgcatcc attcaatggt ccgctcaaca
      421 aacgaatggc ataagattcc aataagtctt gtgataagaa tttatcatta ttttccatga
      481 ataccacccg ctaattccat aagctctagt ttagagtagt gtaacaagac ggaaatttca
      541 tgaccaaatt atgatgacac agaataaaaa aattataatt ccgagaacta ttttaaatgc
      601 ctctatgcat aactttattt acaatttaag gtataaaccc tattccctca atgttaagca
      661 aatataacaa tcactcttat tttaaatttt acacgtaata tttaccctaa atgattttag
      721 agtacaggtg ggcatatgca agtcttttcc agtgatgtct acttcactgt aggcactaat
      781 gcattattag catcccaaaa agaatattac agcgatttag ttgcgttggt tgatcttgga
      841 cattcgttcg ttgttattga tgagcatcaa catcgaaatc tcaaaccaaa tacagaacct
      901 gtaaacatcc ttttgagcaa caatttcatc aggatcaata agaatattac attatctgat
      961 ttaacgcatt tcctggtttc aaatcttcat acacaaaatg tgtattccac acaagaacca
     1021 cttacccatg atgagataga tatcctgcgg ttatgtgttt cttactcgtt aaaacaaata
     1081 gccattatta aaggaattga ttataaaaca atatcatatc acaaaataag agccctaaat
     1141 aaacttaaca taaaaggtac tgtttagttg tttatagcac tctgtgaatg ggataaacat
     1201 tattttaaac tccaatcttg cgtaagagaa agttgatatg tcattagtag gatataatag
     1261 taacatcata ataaccatcg cgctgatatc gttgctctct gcatgcacca gagaaaattc
     1321 attaaaggag aatcaaaata aaagaagtgg cgaatcccaa gcgatagtag atgcaagaca
     1381 gttctttagc gaacacccgg aatatatatc atcagcagaa ctggagcaaa agctatatcg
     1441 cgaatttgca caggttacta cagttccagc ttataaagag atgagtatgt atcaattgct
     1501 tatcatttca caagatcgtt taactcatta aataaggatc tacacgcttt ttgcgtgtag
     1561 attcttataa atttctgact acgacagatg tttaattttt taaattatcg tctttattct
     1621 cgatttttgt gtcttcaccc tcaatgaatt tatcttctga agagagctgt tcatcttgaa
     1681 caggttgccc agcattctcc acatcttcaa gccacagcag aagacgtaat atttcatcta
     1741 tattttcgaa gctaacataa tcgtagcgac gatgggtagc ataatttttt cgtgctaatt
     1801 ttttatctgt gataattggt attccgattt cctttgcata tttccttacg cctaaggcca
     1861 cctcattagt tactcttaca gaaatcaatg gaataggtga aagatgtggc ttaaagtata
     1921 tccctattgc aatgtgagta gggttggaaa tcatcaaacg actattactg acatcagact
     1981 tcaattgctc agaaagaatt tcctgatgcg ctctctgcgt ttagacttaa tttctggatt
     2041 tccttcttgc tccttgtatt ctcttttaac ttcttgttta tccattttca tatctttcat
     2101 aaataaaaaa tattcagcga taaaatcaaa aattaagaca attatcatac tgccgagaca
     2161 atacagtatg agaagg
//
LOCUS       DAZFFI010000286         2174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFFI010000286 DAZFFI010000000
VERSION     DAZFFI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.288"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            299..>2174
                     /locus_tag="ACHJ12_005176"
     CDS             299..>2174
                     /locus_tag="ACHJ12_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4992799.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGYQVYG
                     DNWKSIEIGTTKEVKKKNSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTG
                     ESIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSS
                     EASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNALA
                     NASNALAVGSQSSASGEDSLAIGGQSTTGKKSTVALGQGATASAAEGNVSIGADSVDK
                     AATPVSGATIKLKNGGTIEYKGFAGNNAASVVSVGDTDKERQIVNVGAGAVSASSTDA
                     INGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEVGSKNGGTTGNEVTVIGKGDIDV
                     TVDGNGLSIDGTKLAENIKKDAIKIKTGDGQTLTKKLGEEVEFNDAGPIKTKINNGKL
                     EITAETASFTPDGNGGNGKITVSNGDDDKLVTAKKIADAINQSGWKIKADSENGGKVE
                     GDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGNVTLS
                     SEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKTAIDG
                     V"
BASE COUNT          683 a          379 c          573 g          539 t
ORIGIN      
        1 gaaatgtatc tcatttttac tgccttcgcc agtgcgtcag gcaatggcag gtagtgtacc
       61 tgcattctgc tgtaacgcgt aatgtcgggt attttactta atatgtttta tgtttgcatt
      121 attataaatc actttattgc cttttgtctg ctttttaatg gttcctgttt ttttacttct
      181 ggctttatat aaatggatgc tgaagaaatg aaggcagtcc accttacata atattcatgc
      241 atgattttta tgctattccg actgagtgga agaaaataaa ggagaaaaga ggatatatat
      301 gaacctcaga aaaaaacata tctgtatagc catatcactt gttcttaatc cgttccctgc
      361 atcactggca gcacaaaaca ctaatgtagt tgaagttgat gaaacagata aaggttacca
      421 agtttatggt gacaactgga aaagtattga aataggtaca acgaaggaag taaagaaaaa
      481 gaattcaacc gaccttaatt ttgaccataa ctccccggta gcgaaagggc ataactccat
      541 tgcgattggt accagtgcag aagcaggagg tgaaattaaa gacgaaaaag gttttaagac
      601 aggagaatcc atagctattg gtgccaaggc gaaagcaact aaagaacagt caattgcaat
      661 aggtggcgat accaaagcta ccggatgggg ggctattgtt attggtggag atgatttaac
      721 ccctttgcat ggtgacagat tcggtgataa agaaattccg aataattact cgtcatctga
      781 agcatcagga gacggaagta ttgttgtggg ctcaaggagc acagctgcag gagacctgtc
      841 tgtgtccctg ggctcaataa gctctgccaa cgggacggct gctaatgcac tgggagccac
      901 ggcggaagct aacggggata tggcgactgc ggttggtttt aatgcgctgg ctaatgccag
      961 taatgctctg gctgttggta gtcagtcctc tgccagcgga gaggattccc tggctatagg
     1021 gggtcagagt acgacgggca aaaagtccac ggttgctctg gggcagggag ctacagcctc
     1081 tgccgcggag gggaacgtgt ccatcggtgc tgattcagtg gacaaggcag caactccggt
     1141 ttccggcgct accattaagt taaagaatgg cgggactatt gaatacaagg gctttgccgg
     1201 aaataacgca gcatcagtag tatctgttgg tgataccgat aaagagcgtc agattgttaa
     1261 cgtgggggcg ggtgctgtat ctgcctcaag tacagatgcc attaacggta gtcagttata
     1321 tgcgatagcg aagacgctga aagatgacct tgatgccatt aatccgacgt ggaagttaaa
     1381 agacagcaca ggaaaagaaa aagaggtcgg cagtaaaaat ggcgggacta caggtaatga
     1441 agtgaccgtc atcggtaaag gggatattga tgttactgtt gatggaaatg ggctgagtat
     1501 tgatggcaca aagcttgctg aaaacatcaa aaaagatgcc atcaagatta aaacgggaga
     1561 tggtcaaaca ctaacgaaaa aacttggtga agaagttgag tttaatgatg ccggaccaat
     1621 aaaaacaaaa attaataatg gcaaacttga gataactgca gaaacagcca gttttacgcc
     1681 tgatggaaat ggtggtaacg ggaagattac agtaagcaat ggtgatgacg ataagcttgt
     1741 tactgcgaaa aagattgctg atgccataaa ccagtctggc tggaaaatta aagctgacag
     1801 tgaaaatggt gggaaagttg agggggataa taaagaggag ctaattaatc ccggtgatac
     1861 agttacactg aaagccggta aaaatctcag cgtgaaacaa agtaacggtg attttacatt
     1921 ctctgttatt gatactccgt cattcagcag tgttcagctg aacggtaatg gaaaaaacgg
     1981 aaatgtaacg ctctcatccg aaggtaataa gctgacattg tcaggcggta atggtgccgg
     2041 acaaaatggt gttgttatca gtggtgtggc aagtggattg aataataagg acctggcagg
     2101 cctgaaagca gacgatgcgg aaatgaaaaa tgccgccagt gttgcagacc tgaaaacggc
     2161 aattgatggt gtgt
//
LOCUS       DAZFFI010000287         2165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFFI010000287 DAZFFI010000000
VERSION     DAZFFI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.289"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1399..1938
                     /locus_tag="ACHJ12_005177"
     CDS             1399..1938
                     /locus_tag="ACHJ12_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFO4992800.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFESSLKYSNHDSEQG"
     gene            1945..>2165
                     /locus_tag="ACHJ12_005178"
     CDS             1945..>2165
                     /locus_tag="ACHJ12_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992801.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYDIEYATGINLYMNCE
                     DKEFNLVDVINEDGNKIMFVVSNNEKIQKP"
BASE COUNT          672 a          443 c          494 g          556 t
ORIGIN      
        1 aaaaaaaata gcaaagaaaa aaactccagc acctgtaatt gagctagata aatatcgaaa
       61 aaaatgacca ttgagccagc ggcaggcgga caatcaggag ctacgtgtta acgttctgac
      121 catgattgtc tatcctgcat tgctcttttg ccgcctcaaa atcccttgcg tgtttttgct
      181 ccccgatctc cagaacgacc gagccgccac ggttccggca gcgccttgag cgcaaccgga
      241 aaccgtctgg ggagaacccc agacccccat cgctgtaaca cagacggtct acgaccacct
      301 accacctgaa tagctgcctg ttccgtgcaa ataggctttg cagggggctg acgcggttcc
      361 tggcgacctg aaagcccctg gttaggggta tcggcgcgcc tgacctcctg aaacgctctg
      421 taagcgtttt tcgcgccttc catgccctga cgggcattta aaccccgttt cgtgcgtctg
      481 ggagcttctg agcacgtttt aagggccaat acgtacccaa cattaccgac accagaaaat
      541 cgcgttctgg tgctctggat ggcttacaga acgcattttc gaacagaaca gcaacggaat
      601 cgccgacaac atgcgagagg tggtaccgct cgccgcagcc ccagcgaccg aacgctagtg
      661 agcgagtggg cgaggaagcg gaagagaacc ggaagttaca ttgagcacgt acgcactgat
      721 gtggggtgtc ggggtgaagc cctgaccagg tggggaatgt ctgagtgcgc gtgcgcggtc
      781 cgacattccc acatcctgtc ccgaaatcag gattgttttt tgcacacaat tcctgcaaat
      841 tcagcattgt tacaggtttg tccggcccgt aactagccga tgaaaataat attggctatt
      901 ccgcataaaa attcctctta cttaatttcc cctgaaatct taggggattt ttttgtctga
      961 aaaatccccg tgccgaaagc tctgtagtaa acgctacata aaaaaatcct gaaatggagg
     1021 attcgtcata aagagattcc agaacagatc gatcatcttg ctgtttggaa aagcggaaaa
     1081 atgagcaaaa acgcggtccg taaggcttgc agaggagtaa gccagtatac actccgctat
     1141 cgctacgtga ctgggtcagg gcttcgcccc gacaccccaa aagcagttag gtagtgttaa
     1201 atcatgagca gtaaaaaaac gctttgatag atctttagag caaaaatcga catttctata
     1261 aaaatcatta gcttaagaca ccatcatacg aaacgttatt tgcatgtgta tacgcaaaca
     1321 tgtttgcatg tgttgtttcg tactgtacga tataaatttc gtaaggtacg aaatatcatt
     1381 gcaggtaagg atgcaagtat gttggaacag caatttgaag actttgaaca gcaaaaacgg
     1441 cgagatcatc aggtacaaga tcaatccaaa aacgctaagc aaaaacgaga tcggttgaat
     1501 cctccagact ggtcgcaggt tactaaggct ggttggaata tgttggacgc tatatcagaa
     1561 aaagttccta gagcatttcg gttatattct ttttttgctc agaatataga taaatcatgt
     1621 ggggctgttg tatgtgatca aaaattctta gctgattatt tcggtgttag tttaagaacg
     1681 atccaatatt ggattggaga tttagaaagt attggcgcaa tagttaagat accagtaggt
     1741 atatattatg cctatgcatt agatccttat caggtatggc gtggatataa caatactaaa
     1801 aaatatgcag catttaacgc aaaaacatta actcaaaaag atggagttat agatcgtaaa
     1861 ttgcggctta tgctttctag acgtgaagag tttgaaagtt ccttaaagta ctctaatcat
     1921 gactcagagc aggggtgatg cactatgaga attaaatatg aatcacgttg gagattagaa
     1981 gaactaaaat tagaaatatg cagttcaata gatgagttaa ttaataaata tgatattgaa
     2041 tatgcaacag gaataaatct ttatatgaat tgtgaagata aagaatttaa tcttgttgat
     2101 gttatcaatg aagatggaaa taaaataatg tttgtcgtta gcaacaatga aaaaatacaa
     2161 aagcc
//
LOCUS       DAZFFI010000288         2150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFFI010000288 DAZFFI010000000
VERSION     DAZFFI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.290"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            156..2141
                     /gene="yjcS"
                     /locus_tag="ACHJ12_005179"
     CDS             156..2141
                     /gene="yjcS"
                     /locus_tag="ACHJ12_005179"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4992802.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          569 a          572 c          524 g          485 t
ORIGIN      
        1 tccctcttca gcacaaatgc ctaataaaat ggctcgttcc gctcacactt ccacttatta
       61 aagtcgcaat ttcccaatta tgataacgcc agctttaaat gacgtatttt gcatggatta
      121 catatttacg acacaaatga taaatggaga tttatatgaa taactctcgg ttattccgtt
      181 tgagcaggat tgttattgcg ttaactgctg ccagcggcat gatggtaaat accgcttacg
      241 caacagatga agcgaaagcc gccactcaat atacccaaca ggttaatcag aattacgcca
      301 aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc
      361 cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat cgcggggaag
      421 attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc ctgtggcgtc
      481 agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg taccaggttc
      541 gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt attgtcatcg
      601 acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc caaaatcgcc
      661 cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac tatggcggcg
      721 tgaaaggcat tatctctgaa gctgatgtga aatccggtaa agtgcaggtt atcgcccctg
      781 ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt atgagccgcc
      841 gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac atcggcaatg
      901 gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac aaaaccatca
      961 ccaaaaccgg tgagaagatg attatcgacg gcctggagtt tgacttcctg atgaccccag
     1021 gtagcgaagc gccagccgaa atgcacttct atattccggc cctgaaagcc ctgtgtaccg
     1081 ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg aaaacccgcg
     1141 acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt aacgacgcgg
     1201 aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc aatgattata
     1261 ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac ctggcgaacc
     1321 agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca cttgccaata
     1381 actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg gtgtataact
     1441 tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt caggtggaga
     1501 tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag
     1561 aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag gtgattgccg
     1621 ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa cagctgggct
     1681 atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg
     1741 aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt cgcgggatgt
     1801 cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta
     1861 aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg acgctgaacg
     1921 atagcgtgct taactaccgt aaaacactgc aatcccaagc tgacgcctct ttctacatca
     1981 gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat ctggtaaaag
     2041 cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc gcctgtctgg
     2101 ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg
//
LOCUS       DAZFFI010000289         2146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFFI010000289 DAZFFI010000000
VERSION     DAZFFI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.291"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            509..717
                     /locus_tag="ACHJ12_005180"
                     /pseudo
     CDS             509..717
                     /locus_tag="ACHJ12_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            704..893
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005181"
                     /pseudo
     CDS             704..893
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(896..1084)
                     /locus_tag="ACHJ12_005182"
     CDS             complement(896..1084)
                     /locus_tag="ACHJ12_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4992803.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1619..1760
                     /locus_tag="ACHJ12_005183"
                     /pseudo
     CDS             1619..1760
                     /locus_tag="ACHJ12_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          576 a          510 c          447 g          613 t
ORIGIN      
        1 tatttcttga cctttatcac agaaccatct ccctggatat ccccatcaca ttgcatgaaa
       61 agcaagtttt accaatcttg attttcagtg ttaaataaga ttaataactg taattctgca
      121 aaaaaaagaa caccatatgt agtatgacta cgtattccac tctgttcgtt cacggactgg
      181 catcgaagtc tgaggtgtac tggcaatagc ggatacagtc atttgttctt tttttaagca
      241 ttcatccgat gatacttttc ctgaatgcta ccggagtggt attcccctgc cgtgagggag
      301 ggcgctgacg gttataaaaa tcctgatata tacccattcc cgtatgacag cgatggcgtc
      361 acatctctgt tctaccatct tcacagaaat atttcgttca ttagcattcc ggtattcgta
      421 taaccacctt cggtggagaa agaataaaca atccctggaa tgcgatgagc gcttgaccag
      481 aaaagcatca tattgcttca tggcgcatca gcgaccacag gatgccgcaa aagccgaaaa
      541 acggaccaga gcgcctgcaa tcattcctcg cctcagtatc tgttgcccat ggagtggccg
      601 ataacagatc attgtcggat gagccttatc cccttccttc ccggtggtat ccggatgtgc
      661 tgccagcctt ctcagttaac cgatgttttc cgggcttttt gccatactga ggactgataa
      721 cgtattcctg gtcgtaaaac ccgtcaatat gcgccggggc atcgacaaaa ctgacacagt
      781 acgtccggaa agaacttaac gctgcatggc acgacaatgc tgcctttgtc tttaccaaca
      841 aggctcgttc ccgcataaaa gttctacgcc gggataaaca tggcgtatgg ctgcgtcagg
      901 ccttcactac ttcacaccgg aagtcttcca ccctgacacg aatataccgt ccgggctctt
      961 tcgcaggtgc aaacagtttt tcacattcgg cgaatgtcat ttctgaatcc tggtaacgca
     1021 accactgaac cggcatatta ccgggtttga ttattgtcgc agttatttta tatcgtttcc
     1081 ccatatcgcc tctcatcctg acaacaaaat taccggctga gccatcactc cggtaaagca
     1141 tgcgaaaaaa agtgagcgtc tcagcaatgg ctcatgcgat gattctgtac gggcaccgat
     1201 ggcacctcag aatacaacaa agatatcaga gtaacatgtc tctctcgtta tttttcagag
     1261 ttcttgacac cctccttctg ccacattgtc aacgccagtg gagacttctg ctgtcagttt
     1321 tttcatccat acagagtgac attaattatc ctgaacaaaa cgaaatatcc tgaatatatt
     1381 tattttctcc tggtgccgga gacatgatga tgtacaggct caggtgcggt gtgaatgtaa
     1441 aatggtatgt taattattca tttctgaatg caaactgaat gcaaacttat tccgaattga
     1501 attctcctga caccacatga taaaaatgag gatataataa aatacttctc ttctggatgt
     1561 gccattacct gccgttgctg taatacggcg ggtatttttt actgacatat cgggaagcca
     1621 gcagacgttc gtagctacat ccccgaaagt ggcacactgc aaactataat acgagtaaaa
     1681 catcgatcac cgtagctgat gtgttcaacg atcgtgcact agcgttctgc gattctcagg
     1741 gactgcctgt tctgagaatc ggcgagtccg cgatcggata cgatgcgatt tcgcagtgac
     1801 tcagggattg catctgaaaa ccctccggct atgtcggtaa atatttattt ctatgaaccg
     1861 tacgattcag atagcattcg cccggccccg taagaataat ctccggacaa ataagatttt
     1921 tcctgaaaaa gcgtctagtc ttttgatatc gttgttttta tgctaatcag ggagtatgtg
     1981 tgatgattgt ctgttacgtt attcttgtta accgtccgtt tgaaacagag attgccggga
     2041 aaaagatatt ttttcagaaa aatgacgtcc tgttgacaga taacatcagc cagtcctttt
     2101 tcctgcgcca tcccggtaat gttactgagc tccggattga tgaatg
//
LOCUS       DAZFFI010000290         2139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFFI010000290 DAZFFI010000000
VERSION     DAZFFI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.292"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..104
                     /locus_tag="ACHJ12_005184"
     CDS             <1..104
                     /locus_tag="ACHJ12_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4992804.1"
                     /translation="IVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            107..466
                     /locus_tag="ACHJ12_005185"
     CDS             107..466
                     /locus_tag="ACHJ12_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992805.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(513..905)
                     /locus_tag="ACHJ12_005186"
     CDS             complement(513..905)
                     /locus_tag="ACHJ12_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4992806.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1032..1292
                     /locus_tag="ACHJ12_005187"
     CDS             1032..1292
                     /locus_tag="ACHJ12_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4992807.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1289..1726
                     /locus_tag="ACHJ12_005188"
     CDS             1289..1726
                     /locus_tag="ACHJ12_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4992808.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            1813..>2139
                     /locus_tag="ACHJ12_005189"
     CDS             1813..>2139
                     /locus_tag="ACHJ12_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFO4992809.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRS"
BASE COUNT          602 a          444 c          511 g          582 t
ORIGIN      
        1 taattgttgc aattgcgtat cagcgaggat gggtatttgt taaattcatc ggaaaccaca
       61 aggaatacga tgctattgat gctgatacca tcgaactgga gtaagcatga acatcaaacc
      121 tattcgtaca gagcaagatt atgaagccgc gttgcgtgct gttgaaccca tgttcgacaa
      181 tgagcccgaa attgacacgc ctgaggggga tttttttgag gtgatgtgtt tgctaataga
      241 ggaatatgaa aaaaaacatt atcccattga gccaccatcc ccaattgaag ctataagatt
      301 tcgcatggaa cagcaggggc tgactgtgaa agatttggaa cccgcaattg ggaaaaaaaa
      361 tcgggtttat gaggtgttga atggtaccag aagccttacg ttaccaatga ttcgccgtct
      421 tcataatcaa tttggtatcc ccctggaaag cctggttgga ttataaaatc tgctagtcat
      481 ttgcctgatg ctcgttccag aaaaggaatg catcaggcag ttttgttttt ctgccgcagt
      541 aactcttcaa gtttccgttt atagaaatcg cgtttttgct ccatatcacg aatgatctgc
      601 tctgcgtcgc tttgaggtaa ctcatctaaa agcgatatga tttttcgttg ttgttctgta
      661 agttgcggtt ggttgtcatt actggataca gccattttat cgccgagagt ttcttcttcc
      721 ataaagaacc aatggacggg atgttgtgag agctctgcta atttttccag tttatccatt
      781 cttggcatca cgcctttcaa ccaaccttgc acggattggg gttttacacc aagacgtctt
      841 cccagctctg actggtttat attcaattcc tgcaacacct gctgaaggcg ttttacaaag
      901 atcatcacca cccctcgtaa actagttccg cgatcctaca gaaaaaattg ataagtggca
      961 ttacaaatag aagttgaaat ttaaatttaa atttgtaatc atcggtgtca tcgtaaagtt
     1021 cggagggaaa catgcaaaaa agtactcaag tgaaaatcct gtcaataatg agccaatcag
     1081 aattagggcg tcgtcttggt aaaacaccgc aaaccataag tgggtggttt aaaaaacgag
     1141 tgccagcgga ggaggttata ccagcatgtg aggcgcttga ctggggagta actccgcatg
     1201 aattgcgccc tgataaatac cctaatccaa ccgatggttt acctgttgag tatcaggcta
     1261 acgcacaagc agcggcggga gttgattcat gaaaatcaag catgagcaca tccgcatggc
     1321 gatgaatgcg tggctgcttt atccgagggt agggcgcaaa aaaatcgctg atgatatagc
     1381 gacagcatat tttgagcttg aaatgactta tccaccaatg catgacacct ctacgacaga
     1441 gggtattgga ttgaatatac aaaatatttt tcgctggctt gaaaaggata cgcctgatgc
     1501 tgttgaaaaa attcaggcac taattccggc tatcctgact gttcttccgc gtgaactgcg
     1561 ttatcacctc agtatttttg acactgttga gcgccgtgca ttactggcgg cgcaggaagc
     1621 gttgagcacg gcaattgatg cacatgatga tgctgtccag gccgtttacc ggaaagcgta
     1681 tttcagcgac ggcgggtcat ccggcgagtc tgttgtggtg cattgatatt tatgccagcc
     1741 ccctgctgat tctgttgatt ggggaatcac agaatatcac cagaggatgg ttcgtcacaa
     1801 gatgaggcaa ttatggccgc attaccatac atgcaactgt acatagctga ttacctggct
     1861 gacaccatgc atttgtccgc agaggagcac ggtgcgtatt tgttgctgat gttcaattac
     1921 tggcaaacag ggaaaccaat acctaaaaac aggctggcaa aaatttcccg tctgactaac
     1981 gagcgatggg ctgatgtgga accatccttg cgtgagtttt tttgcgataa cggcgatgaa
     2041 tgggtgcatc ttcggattga ggaagatctg gcatcagtca gggaaaaatt gaccaaaaaa
     2101 tcagccgccg gaaaagcatc tgttcaggcc agaagaagc
//
LOCUS       DAZFFI010000291         2086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFFI010000291 DAZFFI010000000
VERSION     DAZFFI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.293"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            252..1604
                     /locus_tag="ACHJ12_005190"
                     /pseudo
     CDS             252..1604
                     /locus_tag="ACHJ12_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1617..>2086
                     /locus_tag="ACHJ12_005191"
     CDS             1617..>2086
                     /locus_tag="ACHJ12_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4992810.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTG"
BASE COUNT          693 a          399 c          384 g          610 t
ORIGIN      
        1 atagttcatc aggattaaaa cactcaaaga aagaggtaat tttagaatga aattatctgt
       61 gatgattatt aatacactgc agagaaataa aaaacaatct gaagcatgct ctgaaacatc
      121 ctactctgtc actctaatga tggcaccttg ctcttcttat aaagaaggga acaattaaga
      181 atattaaatt ttcaaacttt catcaacttc acatcaagaa attaaaaaat ttaaacatta
      241 atctaaataa taacaacctg atcacataat cgggtacgat tttatccgat aactataatg
      301 ctataactct gggaaatggt tggaatacac cgctgggtgc catctctttt tacgctgccc
      361 gatcgagtag taagttaaat aatgatacca gacacgaagg tacgagctat caggtcgcct
      421 ataataaata tctagtacaa acagccactc gatttagcgt ggccgcctgg cgttatgctt
      481 cgcaggatta caggacattc agcgaccatc tttacgaaaa tgataaacac tatcatcaga
      541 gtgattatga tgatttttat gatattggca gaaaaaatag cctttctgct aatattatgc
      601 aacctttatc caataatctg ggaaatgtat cattaagtgc gctttggcgg aattactggg
      661 ggcgaagtgg aaatgctaaa gattaccaat tcagttactc caataactgg caacacatta
      721 gttatacttt ctctgccagc caatcttatg atgaaaataa taaagaagag gagcgtttta
      781 atctgtttat ctccattcct ttctactggg gggatgatat tgccaaaaca cgtcaccaaa
      841 ttaacttatc gaattcgacc tcattttcca aagatggcta ttcctccaac aatactggaa
      901 ttactggcat agccggtgaa catgatcagt taaattatgg tatatatgtt aatcagcaac
      961 aacaaaataa tgatacctcg cttggtacga atttaagctg gaaaactccc atcgccataa
     1021 tagatgggag ctatagccat tctaaaaacg cctggcaaag tggtggaagt attagtagtg
     1081 gattagttgt ctggcccggt ggtattaata tcactaacca gttatccgat acatttgcaa
     1141 ttctggatgc gcctgggtta gaaggtgcac atattaatgg acaaaaatac aatcgaacaa
     1201 acagcaaagg ccaggttgtt tacgacctga taatacctca tcgtgaaaac catctggtac
     1261 ttgatatagc aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa attattgcac
     1321 cttaccgtgg agcagtttct tatgtgcagt ttacaactga ccaacgtaag ccctggtata
     1381 tacaggcact gcgtcccgat ggttcgccat taacctttgg ctatgacgta ctggatctcc
     1441 aggaaaataa tattggagtc gttggccagg gtagtcgcct ttttattcac gtagatgaaa
     1501 ttcccactgg cataaaagtt gctctcaatg atgaacagaa cttattctgt actattactt
     1561 ttcaacacgt tatcgatgaa aacaaaacat atatatgcca gtaaaaggag tttactatga
     1621 agtggctttt attaatcacc ttgagtctat attcttttat tgtgcaaagt gctccctgtg
     1681 cattgaccaa tgtcggtgaa caacgtggaa catatatact caagccactg agtatgaaag
     1741 gtaatctaac cgccgaaaaa attgaaaaca ccggaaatat cattgagtgt acagacttgc
     1801 aagagacaac caacgccatt aaagttcatt ttttaacaca gaacgcttta aggtttaaag
     1861 tcggaagtcg tcattatatt attaatttcc cctttgaatc tgacggtaaa aaaatagagc
     1921 ttccagggaa gtcatcttat aatttagaag aaatgctatc tgcgataaat ttcaccattc
     1981 catattctat cgcatctgta tcagatagtt ctaacaccgc agacattttt cctggaactc
     2041 cttttccttt gttgttcgaa attgaagtga gttcatgtac agggaa
//
LOCUS       DAZFFI010000292         2086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFFI010000292 DAZFFI010000000
VERSION     DAZFFI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.294"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            224..1465
                     /locus_tag="ACHJ12_005192"
     CDS             224..1465
                     /locus_tag="ACHJ12_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4992811.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLIKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALIRAGTDPQDHERQKREAEARR
                     QGNTFENVATAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGMIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRVVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
BASE COUNT          627 a          505 c          508 g          446 t
ORIGIN      
        1 gaccaaatcg cccaccggat accaccggaa atcaccgaat aagacaggga tagcccgcca
       61 attacttgat tggcgggttt ttttatggtt tttagtcacc gaacatcacc ggatttataa
      121 gctatcagcc ggatttttta gttatacgtt tagttatacg ggctgacgta taactaaagc
      181 cgtataacta aaaccctgtt ttttcgtttg gaatcataag catatggcga gaaaaacacc
      241 cccattaacc acagtacaga tcaaagcagc cagaccagca gaaaaggaat acaccctaca
      301 ggacggcgga gggctttttc tcctgatcaa accgtccgga tcaaaactct ggagattttc
      361 ctactaccga ccatcggaca aaaaaagaat attgctgagt tttggatcgc ttgatgatgt
      421 ttccctggct gatgccagaa aacgccgtag cgagtacagg gcgttaattc gtgccggaac
      481 tgacccgcag gaccacgaga ggcaaaaaag agaggcagag gcccgaagac aagggaacac
      541 gttcgaaaat gtggcaacag catggtacca ggtgaaaatc agccagaatc tggcccccaa
      601 tacgattaaa gacatctggc gatcgctgga aaaatatgta ttcccgttca tcggcaacac
      661 gccaatagac accctgaccg ctcgcaggtt tgttgaggtg cttaccccca tcaaggaacg
      721 tggcaacctg gaaacgctca agcgagtttt acagcgtgta aatgaggtaa tggattacgc
      781 cgccaatagt gggatgattg atgccaatcc ggctatgaat gtgcgtaagg cgttcccctc
      841 ccctgtaaaa aaacatatgc caacaatccg ccccgaacag ctgccggagc ttatgcaggc
      901 tttatcagta tcggcaacag aacggcagac cagattactg attgaatggc agttactgac
      961 cgtaacccgt cccgttgaag catcgtcggc acgctgggaa gaaatcgaca tggaggcgca
     1021 acgctggacc atacccgccg gacgcatgaa gatgcgccgc gaccacgtta tcccgctttg
     1081 tgaccaggct atggcagtgc tggaggctat gaaaccaatc agccaccacc gcgattatgt
     1141 tttcccgagt ctgaaagacc cacggcagcc aatgaacagc cagacagcaa acgcagcatt
     1201 acgacgcatg gggttcgctg gcgtgctggt gtctcatgga ttacgcgcca tattcagtac
     1261 agcagcgaac gaggaaggat tcgagccgga cgtaatagag gcagcacttg cccacgtcga
     1321 caccaacgaa gttagacggg catacaaccg gagcaactac atagaaaaac gcgtggtgct
     1381 tatgcgctgg tggggcgaat ttgtcgaggc tgcggcgacg ggcgtaactc tcgccagtgg
     1441 taaaaggggt atccgagccg tttagctgta cagaaaacca gtaaaaacta cgaaaaccat
     1501 ttaaaaccgt cgtataattg catcaaattt aacgacgagg ccttgaaaaa tgaaaccgtt
     1561 aagatgcaaa caaatttcag atgcaattgc gacgggctgc aactggccct gatggttcag
     1621 catgaatttt ggtcaaccta cgatccggag gacagaacga cggccccatc aaaaaaacag
     1681 gtagtagatt tcctggtatc ccgtggagct tccagaaatc tggctgtaag tattgataag
     1741 gttgtacgtc cggcatccat gaagaccgga ggcaggccaa aaaaatggcg gtaacaatcc
     1801 tgaaagcggc agaaatgccg ctttttttat aaatcccttt caaatcatca atataaaaaa
     1861 cggtgtatac cgtttaaaaa cggtgagtac tgtttttccc tgcatccgat gatttaccgt
     1921 atttctcacc ggaatacacc ggattaatga ggtaaatcac gatggaagca atcaaaaaag
     1981 tcatctttcg ccaagaagta aaaaaactta ttcacatcaa ggcagacagc acgctgcaaa
     2041 gcatgatcaa cgccggagaa ttcccgcagg gttttcgtgt cggttt
//
LOCUS       DAZFFI010000293         2071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFFI010000293 DAZFFI010000000
VERSION     DAZFFI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.295"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..2059
                     /gene="cirA"
                     /locus_tag="ACHJ12_005193"
     CDS             65..2059
                     /gene="cirA"
                     /locus_tag="ACHJ12_005193"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4992812.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSAGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
BASE COUNT          620 a          376 c          533 g          542 t
ORIGIN      
        1 atttatggaa gtcagtgtgg gtaaatcttg caacattgaa tacaaattat ggatataatc
       61 agaaatgttc agatttaacc cttttattcg tcatcttctt tcaaccagca taggtgcatc
      121 gttattttca tttcctgtgt tcacttctgc acattcgaca cttccggacg ctgctgaaga
      181 tactctggta gttacagcat ctgctgttga acagaatctg aaagacgcac ctgccagtat
      241 cagtgttatt agccaggatg atttatcccg taagccagtc cataatctgg tggatgtttt
      301 aagggatgtg cccggggtgc agattaccac cgatgataac agaaaactcg taaatataag
      361 gggactgggc ggaggataca ccctcattct tgtggatggt aaacgggtca gttcccgcac
      421 agctatgttc cggaataatg actttgatct gagctggatc cctgtttcca ctattgaacg
      481 tgtagaggtc gtgcgtggcc ctatgtcatc actgtatggt gcggatgcga tggggggcgt
      541 cattaatatc attactaaaa aaacaggtaa ggaatggtca ggtgtcatct cgggggatat
      601 gacgttacag gaacacagag accgtggcga tacttatggg ggacagttca gtgcaggtgg
      661 gcctttggtt gaaaaccttt taggaattaa agtttacgga gctttaagtg gccgggataa
      721 agataccgga aaacaaaaat cggcagatgg acaggaaact acacgtgctg aaggttttac
      781 aacccgtaat ggtggcgttg actttacgct tactcctctg gagaatcacg agttcagcgc
      841 agggtattct tttgaccgtc aggaccggga gtctgaaggt agtagtgata aaaaccggat
      901 ggaacgacag aattattctg ttggccataa tggacaatgg ggattaggga acagtgaact
      961 ccgattttac agtgaaaaag taaataatct gaatacaggt aatacacatc cgataacttc
     1021 atggaatgag gttattgatg gaaaatatgt attcccattc actgattata atcatatcgt
     1081 gatattaggt ggtgattatc gcagggagaa aataagtgat cctgtccacc ttaccggaga
     1141 gaaaggttca gaaaattcga caagtcagta ttcacttttt gcagaagacg aatggcgttt
     1201 gttcgcgccc ctggctttga caacaggggt tcgtatggat aagcacgaaa attatggaac
     1261 caactggagt ccaagagcat atcttgtatg gaatgcaacc gattctgtta ctgtaaaagg
     1321 aggatgggcc agagcattta aagctccgac cctgattcag caaagtgaag catggctgac
     1381 gaactcatgc aaagggggat gtcagatcat cggtagcccc gacctgaaac cggaaacaag
     1441 tgaaagtatt gagggtggtg tttattacag cggacaggaa ggactcctgg aaggtgtgga
     1501 agcaagtgtc actgtgtatc gtaatgaaat tgatgacatg attgcgaaca aacgcagtaa
     1561 gaaactgaaa gaggcaatga cgctgcctaa ctttgtcggg ataacggatg atggacggcc
     1621 tgttttcaga tactttaata taaataaagc taggatacag gggcttgaat cagaactgaa
     1681 aatcccgctg ggagagcagg tgaaactgtc tctcacctat acctatacag atggacggga
     1741 tttgagtagc ggagaagata agccactgaa tgacagtccg tttcataatg caaacggaag
     1801 aattgactgg actcctgtgg atgactggtc tttttacgta tcagctaatt accatgggga
     1861 aacccgggca caatcttccg gcacagaaac gcaggggggg tatacattat gggatgccgg
     1921 agcttcctgg aaggctacga aaaatataac tgtccgttca ggtatattaa atgttatgga
     1981 taaagattta gaacggaatg attacagtta taccgttgac gggagaagat attttctgtc
     2041 aatggattat cgtttttaat agtaaaccga a
//
LOCUS       DAZFFI010000294         2054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFFI010000294 DAZFFI010000000
VERSION     DAZFFI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.296"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..655)
                     /locus_tag="ACHJ12_005194"
     CDS             complement(<1..655)
                     /locus_tag="ACHJ12_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4992813.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGRGNPMMINAHNRAQAVFLC
                     VKHSHIQIMVGRAGQLQGWPAPLVTGISTPVRLTTFMVVENLGGELFKLINEAAIMAT
                     TLTLSHPDVTIENGRAVTTSVAVAEFFHKLHKNVIQKIEALECSDEFTELNFQPSEYT
                     DTTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     "
     gene            1363..1809
                     /locus_tag="ACHJ12_005195"
     CDS             1363..1809
                     /locus_tag="ACHJ12_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992814.1"
                     /translation="MDRDDILDRVLYGYFLEQLFSVATGRLDKLLSVVSIILGSSVIG
                     GFIPEVSGVLIVVIATVQTIYGFGQKSGNAMRKSAEYLQLYDDAEKYSDSELKIQLKL
                     LEKTDDNIWSSLKDIAILKTRIKIGISVEQQEKLSAKSKLMRFLCG"
     gene            complement(1908..>2054)
                     /locus_tag="ACHJ12_005196"
     CDS             complement(1908..>2054)
                     /locus_tag="ACHJ12_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4992815.1"
                     /translation="TLNLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTAAGARRWLA
                     RNSD"
BASE COUNT          535 a          464 c          503 g          552 t
ORIGIN      
        1 tcattttgtc gggagacggg gcgttattct ggcgcagttc tttctccatg cgatcgaact
       61 cagcgatgta ggcttcttta aatgcagcgg cttttttgcc ggtgaagccc atcaccagga
      121 aaacgaagcc gtttttggtg atttggtaca tagggagttt gcgcccggtt gtgtcggtgt
      181 attcgctggg ctgaaaatta agctcagtga attcgtcgga acattccaga gcttcaattt
      241 tttgaatgac gtttttatgc agtttgtgga agaactctgc aactgcaaca gacgtggtga
      301 ctgcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtaagggta gtagccatga
      361 tggcagcctc gttgatcagt ttaaataact caccaccgag gttttccacg accataaagg
      421 tggtgagacg tacaggggtg gaaataccgg tgaccaacgg agccggccag ccttgcagct
      481 gccctgcacg ccccaccata atttgaatgt ggctgtgctt gacgcataaa aaaaccgcct
      541 gagcgcggtt atgcgcgttg atcatcatcg ggtttccacg cccggcaccc gttttatgag
      601 gtgcaggtgc actataattc cacccgttct ggttttcaat agctacattc aacattttct
      661 cttacctttc atcaccgaag tgaactttgt tgatgcagtg cctggtgcct ccaggtgacg
      721 ttaaccagtt aacaattaac gccggattgt tagttgatgt ctgttacgcc agtaaaagac
      781 cgcttgtttt aactgttccg cgtgcgctta gccgcattca ccgcatcaca aaattcactt
      841 taaaaagggt ggcagagcag tcacggagta gaactgatgc caccaaagac tacacatggg
      901 tattgtggcg gggctgtcac ttaatcgtat ggtcaacctg acaacccggt gcattttctg
      961 gagcaatgga ggaaacccca gccatactta ccgccgcgcc atttcgcgga ttgccacaac
     1021 cggaagcgca cggtcgaaga aatctaacga caagccttct aagggaaaga gcttcgccgt
     1081 acgctttcgc gttgtgtgcc tgcttttaac cacgtcaggc gaggtggttt ccgtcgttcc
     1141 ccaacgacag gaaatctgta taatctggat atccccaaca acaggaaggt gttttatatg
     1201 gtggttcaaa agaattgttc aggtactggt aagcctgcgg acagggcgga cattccgaat
     1261 accaggaaac atatacctga gaaaaacata cgacctaaac catctccagc tccttcagag
     1321 gagaggggga gcagcaacaa tcaagcaagc aggtgatgat atatggaccg ggatgatatt
     1381 cttgacaggg ttttatatgg ctattttctt gaacaactat tctctgtagc gactggtcgt
     1441 ctcgataaac ttctctcagt ggtgagtatt atcctcggtt catctgtcat tggtggattt
     1501 attcctgaag tttctggagt tctcattgtt gtgatagcaa ccgttcaaac gatttacgga
     1561 ttcggacaaa agtcaggtaa cgcaatgaga aaatccgcag aatatctgca gctttatgat
     1621 gatgcagaaa aatattccga ttcggaattg aaaattcagt taaagcttct ggaaaaaaca
     1681 gatgataata tttggtcgtc gcttaaagat atcgcaatct taaaaacccg gatcaaaata
     1741 ggaatctccg tagaacaaca agagaaactg tctgcaaaat ccaaattgat gcgattcctt
     1801 tgtggttagg aatacccaga ttgttaaaga tcatgccgga tgcttacccg tgtccggcga
     1861 acgtattcca cttcgcctgt ggagaactct ttaattacta accttcatca gtcagagttt
     1921 cttgctaacc agcgacgcgc gccagctgcg gttttaaacg ttttgcttct ggtatacgtc
     1981 atcgcggtaa acgtgccgtc ctggttggga aacacgccgc ataccagaga ttcgttgttg
     2041 ccaagattga gcgt
//
LOCUS       DAZFFI010000295         2022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFFI010000295 DAZFFI010000000
VERSION     DAZFFI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.297"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2022
                     /locus_tag="ACHJ12_005197"
     CDS             <1..>2022
                     /locus_tag="ACHJ12_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4992816.1"
                     /translation="ETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETTSKGDR
                     NPSSVRLLIQLERNGHWVTEKDITINGKTTSQYLMSVILNNLPERPFNIRMVRETADS
                     TTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIRGRIIQVP
                     SNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAI
                     GQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQD
                     RPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDP
                     DAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFMLGSQGLRHTPGDI
                     IEICDNDYAGTLTGGRILSMDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDIT
                     AHPAPDRIQVSVLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGATFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFS
                     LRLTSGSGESSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRIN
                     APAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETS"
BASE COUNT          434 a          578 c          643 g          367 t
ORIGIN      
        1 agaaaccgca ctgggcgtgg aggtgacgaa ggcaaagccg gtgacgcgca ccatcacgtc
       61 agcgaacatt gaccgtctgc gggtcacctt cggggtgcag tcactggtgg agaccacctc
      121 aaagggtgac cgtaatccct cttctgtccg gctgctgatt cagcttgagc gtaacgggca
      181 ctgggtgacg gagaaggata tcaccattaa cggcaaaacc acctcgcagt acctgatgtc
      241 ggtgattctg aataatctcc ctgagcgccc ctttaacatc cggatggtca gggagacggc
      301 ggacagcacc acggaccagc tgcagaacag aacgctgtgg tcgtcataca ccgaaatcat
      361 cgatgtgaaa cagtgctacc cgaacacggc gattgtgggg ctgcaggtgg atgcggagca
      421 gtttggtggc cagcagctga cggtgaacta ccatatccgt ggtcgcatca ttcaggtgcc
      481 gtcaaactat gacccggaaa aacgcaccta cagcggtatc tgggacggca gtctgaaacc
      541 ggcatacagc aataacccgg cctggtgcct gtgggacatg ctgacccacc cgcgctacgg
      601 catgggaaaa cgcctggggg ccgcggatgt ggacaagtgg gcactgtatg ccatcgggca
      661 gtactgcgac cagacggtcc cggatggttt cgggggcaca gagccgcgga tgactttcaa
      721 tgcgtacctg tcacagcagc gtaaggtctg ggatgttctg ggggatttct gctcggcgat
      781 gcgctgtatg ccggtatgga acggccagac gctgacgttc gttcaggacc gtccgtcgga
      841 tgtggtgtgg ccgtacacca acagcgatgt ggtggtggat gataacggcg tggggttccg
      901 ctacagcttc agtgccctga aggaccggca cacggcggtg gaggtgaatt acaccgaccc
      961 gcagaacggc tggcagactt ccactgaact ggtggaagac ccggacgcca tcctgcgcta
     1021 cgggcgcaat ctgctgaaga tggatgcgtt tggctgtacc agccgcggtc aggcccaccg
     1081 tgccggactg tgggtgataa agacagaact gctggaaacg cagacggtgg atttcatgct
     1141 cgggtcacag gggctgcggc acacgcccgg tgacatcatt gaaatctgtg ataacgacta
     1201 cgccgggacc ctgaccggcg gacgtatcct gtccatggat gccgccagcc gtaccctgac
     1261 gctggaccgt gaggtgaccc tgccggagac agggacatcg acggtgaacc tgattaacgg
     1321 cagcggtaag ccggtgcgcg tggacatcac cgcacacccc gccccggacc ggatacaggt
     1381 cagcgtcctg ccggatggcg tggagacata cggtgtgtgg ggactctccc tgccgtcact
     1441 gcgtcgtcgc ctgttccgct gtgtttccat ccgggaaaac acggacggca cctttgccat
     1501 cacggcggtg cagcatgtgc cggaaaaaga agccatcgtg gataacgggg ccacctttga
     1561 gccgctctcc ggttcactga acagcgtcat cccgccggct gtgcagcacc tcacggtgga
     1621 ggtgagtgcc tcagacggcc agtatctggc gctggcgaaa tgggacacgc cgcgggtggt
     1681 gaagggcgtg cgcttcagtc tgcgcctgac cagtggcagt ggtgaaagca gccgcctggt
     1741 gaccagcgct atcactgccg acacggagca ccgtttcagt ggcctgccgc ccggggaata
     1801 caccctgacg gtcagggcga taaacagcta cggccagcag ggcgaacctg ccaccaccac
     1861 gttccggatt aacgcaccgg cagcaccggc cagcattgaa ctgacgccgg ggtattatca
     1921 gataacggcg gtcccgcatc ttacggtgta tgacccgacg gtacaatttg aattctggtt
     1981 ttcggaggca aaaatcgcag acacatctca ggtggaaacc tc
//
LOCUS       DAZFFI010000296         2018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFFI010000296 DAZFFI010000000
VERSION     DAZFFI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.298"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..412
                     /locus_tag="ACHJ12_005198"
     CDS             230..412
                     /locus_tag="ACHJ12_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4992817.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            336..722
                     /locus_tag="ACHJ12_005199"
     CDS             336..722
                     /locus_tag="ACHJ12_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4992818.1"
                     /translation="MIKGPQRKSAPVSQPEANPKSPLLLNNPFQRENPMSEVKPLVTA
                     DAVKDALRSEDVRGALKQKVRQTFEAQIEADVDAILDTILGEQPGEQPEPEPQPEDIT
                     SENGDIQPESPVVDATEAQPEPGTML"
     gene            798..1013
                     /locus_tag="ACHJ12_005200"
     CDS             798..1013
                     /locus_tag="ACHJ12_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4992819.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGD"
     gene            1017..1574
                     /locus_tag="ACHJ12_005201"
     CDS             1017..1574
                     /locus_tag="ACHJ12_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992820.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSTLMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGVKITISENGKVTPPSHQ
                     HSEELIEFAIDYLKNNKKQGLMKRIGRCMGYLQVAAEIKALASGADKDSVVREALLRD
                     FDNPPFKKVPAYWLHPGLTYLKVRI"
     gene            1611..>2018
                     /locus_tag="ACHJ12_005202"
     CDS             1611..>2018
                     /locus_tag="ACHJ12_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4992821.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGAC"
BASE COUNT          557 a          435 c          573 g          453 t
ORIGIN      
        1 catatcaaag gcggcgacat atacgttgct gattgacagt gtgaaggtgg aagtgaacaa
       61 cgcggatgcc gcggcatgat aaaaaaggcc gccagcaatg aaggaaaggg atgaactttg
      121 tccactacat gaatgctggc ggctggtaaa acgaccatga tgacaccgaa gtctgcggtg
      181 tttttctaca aaaataagta aatcatgtca actatcacgg tgagaaacca tgacatttct
      241 gaaccagtta atgctgtact tctgtacggt ggtctgtgtg ctgtatcttc tttcgggtgg
      301 gtacagggca gtgcgcgatt tctggcgcag gcagattgat aaaagggccg cagagaaaat
      361 cagcgccagt cagtcagccg gaagcaaacc cgaagagccc attactcctt aacaacccct
      421 ttcaacgaga aaaccctatg tcagaagtaa aacccctggt cactgctgat gcagtgaaag
      481 acgcgctgcg ctcagaagat gtgcgtggag cactgaaaca gaaagtccgc cagacatttg
      541 aagcgcagat agaagcagat gtggatgcga ttctggatac gatactggga gagcagccag
      601 gcgaacagcc agagccggaa ccgcaaccgg aagatatcac ctcagaaaac ggcgatattc
      661 aaccggaatc accggtggtt gatgcgactg aagcacaacc cgaaccgggc accatgctgt
      721 aagggtgagt cagggccatc agtaaagtac tgctggcctt tttcatgttg tgagcttccg
      781 tgtgcgggag acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca
      841 cgtcagcggt gggaacgggc tactggttcc tgcagttgct ggacagggtt tccccgtctc
      901 agtgggcggc aataggcgtg ctggggagtc tgctgtttgg gctgctgaca tatctgacga
      961 acctgtattt caagattaaa gaagaccggc gtaaggcggc acggggagat taggtgatga
     1021 accatgaaga aatgaatcag cgcttcagtc gtctggaaaa tgaaattgct gaactgaata
     1081 aaaaactgtc gacgctgatg ccttctgaag atgaaaaaaa acgccgcgat gagcagtttg
     1141 ctgcgtttga cgattattgt cggaaagtga tgagcagaaa tctcgcagag tgtttcagta
     1201 ttcataatga taatttcagt gagctggaat gggagtgtaa ccggccatcc tttgttgtat
     1261 ccggtgatgg tgtgaaaata accatctcag aaaatgggaa agtaacacct ccatcgcacc
     1321 agcacagtga ggagctcatt gaatttgcca ttgattacct gaagaacaat aaaaaacagg
     1381 ggctgatgaa gcgtattggt cgttgcatgg ggtatctgca ggtagccgct gagattaaag
     1441 cgctggccag tggtgcggac aaggattcag ttgtgcggga ggcacttctt cgtgattttg
     1501 ataatccgcc ttttaaaaaa gtgccggctt actggcttca tcccggactg acttatctga
     1561 aagtgcgtat ttagtgggcc tgggacagcg gctgaatatt taatatatcc atgaacacaa
     1621 aaatcaaata cggcctgtcg gctgccgttc tggcgctgat tgccgcaggg gcttctgcgc
     1681 ctgaaatcct cgaccagttt ctggatgaaa aagaaggcaa ccacaccacg gcataccgtg
     1741 atggcgcggg tatctggacc atctgccgtg gagccacccg ggtggatggt aagcctgtta
     1801 ttcctggcat gaagctgtcg aaggggaaat gcgaccgggt taacgccatt gagcgtgata
     1861 aggcgctggc atgggtggag aaaaacatca aagtgccact gactgaaccc cagaaagcgg
     1921 gtattgcgtc attctgtcct tacaacattg gcccgggtaa gtgtttcccg tcgacgtttt
     1981 ataaacgaat taatgcagga gatcgcaggg gagcgtgt
//
LOCUS       DAZFFI010000297         2006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFFI010000297 DAZFFI010000000
VERSION     DAZFFI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.299"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..1676
                     /locus_tag="ACHJ12_005203"
     CDS             3..1676
                     /locus_tag="ACHJ12_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO4992822.1"
                     /translation="MLNSSISLILASGLMMSTANAADDVKLKATKTNVAFADFTPTEY
                     STKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAIEAAKKSTP
                     TLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGIPLEETFLS
                     ELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQNRGFNYFM
                     GYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPFMIYLAYSA
                     PHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQYDNTIILF
                     TSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKLVSSMDFYP
                     TALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSHWWDEENTA
                     FWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLALYDINDLQQ
                     TKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            1745..2002
                     /locus_tag="ACHJ12_005204"
     CDS             1745..2002
                     /locus_tag="ACHJ12_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992823.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
BASE COUNT          669 a          421 c          395 g          521 t
ORIGIN      
        1 aactgcttaa ctcatcaata tcacttatat tagcatctgg tctgatgatg agtactgcaa
       61 atgcagcaga tgatgttaag ctcaaagcaa caaaaaccaa tgttgccttt gcggacttca
      121 cgccgacgga atacagcact aaaggtaagc caaatatcat tgttttaaca atggatgatt
      181 tagggtatgg acaattgcct tttgatgata gttcatttga ccctaaaacg atggataacc
      241 gaaatgttgt tgatacatac aaaataggta ttgataaagc aattgaagct gcaaaaaaat
      301 ccactccaac attagcggcc ctgatggacg aaggtgtaaa attcactaac ggctatgttg
      361 cacacggtgt ctctggccct tcacgtgccg caatcctgac ggcgcgagct cctgctcgct
      421 tcggtgtata ttcaaattct gatgcacagg atggtatccc tctggaagaa acattcctgt
      481 ctgaattact tcagaatcat ggttattaca ctgcagcaat cggtaagtgg cacttatcaa
      541 aaatcagcaa cgtaggtgtg cctgaagaaa aacaaacccg tgactaccac gataacttca
      601 ccaccttctc cgcagaagaa tggcaacctc agaatcgtgg cttcaactac ttcatgggtt
      661 atcacgcatc cggcgtttca ctttataact ccccgtcatt gttccgtaac cgtgaaaaca
      721 ttaaagctga agggtacatt agtgatcaat taactgatga agcaattggt gtaattgacc
      781 gtgcaagcaa actggaacag ccatttatga tttatctggc atacagtgca ccacacctgc
      841 ctaacgatca gcctgcgcct aaaaactatc aggagaaatt caacactggt agcaagactg
      901 ctgataacta ttatgcgtca atttactccg ttgaccaggg ggttaaaaag atcctgaata
      961 cacttaagga aaaaggtcag tacgacaaca ccatcattct gttcacctct gataacggtg
     1021 ctgtaattga tggtccactg ccactgaatg gtgcgcaggc tggttacaaa ggccaaccgt
     1081 atccgggtgg tatgcataca cctatgttca tgtggtggaa tggcaagctg aaaacgggtg
     1141 attatgacaa actggtttca tcaatggact tctatccgac cgctttagat gccgcagata
     1201 tcaagatccc tgcttctctg aaactagatg gtgtatcatt attgccatat gtacagaaag
     1261 gagctgataa gaaatctgca gctggcgaac ctcacaaata tctgtcctgg attacctctt
     1321 attctcactg gtgggacgaa gagaacactg cattctggaa cggatatcat aaattcgtga
     1381 gaaaagagtc tgatgattat ccgcacaacc cacatacaga agacttaagc cagtttgcat
     1441 acactgtaag aagcaacgat tattctctgg tttacaccta tgcagagaat aaactagctc
     1501 tgtacgatat caacgatctg caacaaacta aagatctctc tgcatcccat cctgatgtag
     1561 ttaagaatat gaaggctgtt atgattgact tcatcacgtc aagtcaaaaa ccgatcagtg
     1621 atatcaacca agccaaattt gagaatataa aaaaatcttt aactaacgtg aaatgataat
     1681 aagcagggag tagatatatc tactccctgg atatagggaa ataaatcagt tagagcaata
     1741 aataatgaac aaacacgaaa ataaaaaata ctgttcatct gacgagattt ggtgtacaca
     1801 acgtaaggag ttaatcaaat ctgttcttga aacaaagaaa gcttctgatt tagaaaaaca
     1861 gagtacagat atagctcaac agtatttatc agagacgatt cagttttcta ttgatgattt
     1921 tatcaaaaat ataaaaagaa aagttaaatc ccgaggttcc aggcctcccg ccaaacgtaa
     1981 aaacactcac aagggaacat agattc
//
LOCUS       DAZFFI010000298         1965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFFI010000298 DAZFFI010000000
VERSION     DAZFFI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.300"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..493)
                     /locus_tag="ACHJ12_005205"
     CDS             complement(<1..493)
                     /locus_tag="ACHJ12_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4992824.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLD"
     gene            complement(502..1269)
                     /gene="sctT"
                     /locus_tag="ACHJ12_005206"
     CDS             complement(502..1269)
                     /gene="sctT"
                     /locus_tag="ACHJ12_005206"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO4992825.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1271..1531)
                     /locus_tag="ACHJ12_005207"
     CDS             complement(1271..1531)
                     /locus_tag="ACHJ12_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4992826.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1541..>1965)
                     /locus_tag="ACHJ12_005208"
     CDS             complement(1541..>1965)
                     /locus_tag="ACHJ12_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4992827.1"
                     /translation="FNNVASVVNFVETGMSGYKSYLIKYSEPELVSFFEKIQKVNSSE
                     DNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMM
                     SPVTISTPIKLILFVAMDGWTMLSKGLILQYFYLSINP"
BASE COUNT          691 a          397 c          320 g          557 t
ORIGIN      
        1 catcaagagt tttaaaaagc agtgatttat gattactcca aaatactttg atcgccaatg
       61 caaaaaaaac aatataaaga attgctttaa caaattcttt tacggttttt aagccaaata
      121 tacgctttaa gccatttact ggattcaatg aatcaaactt aaatttaaca gcttcagttg
      181 ctaattgcat tttactttga aaccaggaga caagtattgt tgtgaacatg caaaccagca
      241 aaaaaggaat gttcgttttc aaccaaccaa cccccatagc tttaaaatag tcccaaatat
      301 ctggtttagc gttatgatca aggatgtatt caagaatcga cataatgtga tgcacatcaa
      361 aaacatatcc gagatataaa gtacccacaa gcattattac agagatggtt aagtctctac
      421 tttttagaat ttgccctttt ttagaagcat cctggagttt cttttgtgtt ggcttttcag
      481 ttttattggc catgaaacac tctatttctc aatgtatagt tgcaattgtt ctgggaaaaa
      541 ggataacggc attactcgtt cagccagtat tggggcaaaa taaataatta atattaaaaa
      601 tgccagcgca cttttgacag tcagtgaaat ggcaaaagca tttagttgtg aagcataaca
      661 tgccagcaat cccaggacag cctcaccgcc cagcataacg gcaatcacgg ggcttgcgta
      721 aacgatagta tgagtcatta tgttattaat atatgagaat aaaggctcta atttaggaaa
      781 attaaaattg cctgaaggcc ataaattata actttgccaa agagtttcca gaataaagtt
      841 cattccacct ttagtaagat aaaccgcagc agagaaaagg ttaaataatc ttgctaattc
      901 cgaggtatcg acacctgtag ccggatccaa agtactactt aatgttgcac cgcgttggtt
      961 atcgataaag ctacctatag caaggaaaat ccaaaacggg gatgctaata agcatgcaat
     1021 aaacagccct atgataattt ctctggcagc gaacaaagca atatcgattt catttaagtt
     1081 caataattca tagtggtaat gctgaactaa tgcacatgat accactatta ttattgggtg
     1141 acgaactact gttggtatat tcccactggc gaaaaatggt aacaaataga aagttggtgc
     1201 caatcggcaa aaaccgagcg ctgtagcagc cagcagacta tgcaactgat agagtatcgc
     1261 ctcccccatg ctatcctctc gcgaatgcta aattcagcat ttcgatacca aatgaatata
     1321 atttttcacc ataccacccg ctcattaaaa aaaagcaaat acttacgcac aataatttca
     1381 cgccaaatgg caacgtctgt tcttgcaact gcgttaccgt ttgaaaaagc cctaccaata
     1441 aaccaacaaa tgtcgcaact gcaataggtc ctgcagacat aactaaaatc agatacagcg
     1501 ccctattccc cgcaaaaaca atatcgtcca tattttttcc tcagggatta atggataggt
     1561 aaaaatattg taaaatgagc ccttttgata acattgtcca accgtccatc gcgacaaaca
     1621 aaattaattt tatcggtgta gatatggtta ccggactcat catcatcata cctaacgtta
     1681 acagaacgct ggatataacc agatccacta cgacaaaggg taaatagata taaaaaccta
     1741 taataaatgc actttttatc tcactcaatg catatgcggg cagtaacgag aaaattgaaa
     1801 tattatcatc atcgattatc tcttcattgt cttcagaact attcaccttc tgaatctttt
     1861 caaaaaagct aaccaactca ggttcagaat attttatcaa atatgattta taaccactca
     1921 tgcctgtctc aacaaaatta acaaccgatg ctacattatt gaatg
//
LOCUS       DAZFFI010000299         1958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFFI010000299 DAZFFI010000000
VERSION     DAZFFI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.301"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1958
                     /locus_tag="ACHJ12_005209"
     CDS             <1..>1958
                     /locus_tag="ACHJ12_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4992828.1"
                     /translation="LAENARNAGELGRFMSVARVAAGGGVAVLAALAAAAWQAEQADR
                     ALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRI
                     MGETSQRWLQTVGDYAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERT
                     KGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGAL
                     SLITDVVAGTVEQVKLLLVQGDLALAEFIQSTWETTKNVPGVGALFGELAEENRVFIE
                     KTKRDELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKH
                     TSPKTRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLK
                     EAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAARFEEQQSA
                     KQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQALARVTGALKQTWADEDMLRGDW
                     LAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSM
                     MTEILLKQAMVGIVGRIGSAIGGAIGGAGASASTGTAIQAAAANFHFATGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYR"
BASE COUNT          466 a          458 c          677 g          357 t
ORIGIN      
        1 ctggctgaaa atgcccggaa tgccggtgaa ctgggacgat ttatgtcggt ggcccgtgtg
       61 gcggcaggtg gcggggttgc cgtactggcc gcgcttgctg ccgctgcctg gcaggcagag
      121 caggctgacc gggccttgtt gcgttcactg accctgaccg gaggggcggc tgccaccaca
      181 acggcagaat tgtggaaaat ggccggggtg atcagcgatg aagccggtgg tggtatcaga
      241 caggcggcag aaaatctggc ccgtctggca gaaagcggga aatataccgc cgggcagcta
      301 cggatcatgg gggaaacctc tcagagatgg ctgcagacgg tgggggacta tgccgggaag
      361 gtggaaaaag cctttgaagg gattgcagca gatccggtga aggcgctggc ctccctgaat
      421 cagcagtata acttcctgag cgtttcccag ttacgccata ttgatgagct tgagcgcacg
      481 aaaggtaaac aggctgcggt aacggaggcg atgtccctgt ttgcggatgt catgaatgca
      541 cgtctggagc aacttgataa agcggccacg ccggtggaaa aaatctggga cgatgttaaa
      601 acctggactt ctgacgcatg ggcatggata ggtgatcata ctctgggggc actcagtctg
      661 atcactgacg tggtggccgg aaccgttgaa caggtgaagc tgctgcttgt gcagggggat
      721 ctggcgctgg ctgaatttat tcagtcaacc tgggaaacga caaagaatgt gcccggcgtt
      781 ggtgcgttgt ttggtgaact ggcagaagag aaccgcgtat ttattgagaa aacaaaacgc
      841 gatgaactgg cgctgagaaa atccattgcg gaacgggatg cgcgtatacg tcagggggaa
      901 atggggtaca tcaaccgttc gcgtgcaaca ggcgtcagca aaggtccggg gcagcaggaa
      961 gccgtcagcc gtctggctga agagctgaca ggtaaaaagc atacatcacc gaaaacgcgc
     1021 tctgccgggg agagggaaga ggagcaggca agagaggctc tgcttgccct tgaagctgaa
     1081 ctcaggacgc tggaaaaaca cagcggtgcg aatgagaaaa tcagccggca acgccgtgat
     1141 ttatggaagg cggaaagtca gtatgcggtc ctgaaagagg ctgccacgaa acgacagtta
     1201 tccgggcagg aaaaatccct gctggcccat gagaaagaga cgctggagta caaacgccag
     1261 ctggctgagc tgggcgacaa ggttgaatac cagaaacggc tgaatgagct ggcacagcag
     1321 gcggcacggt ttgaagagca gcagagcgcg aagcaggccg ccatcagcgc aaaagcccgt
     1381 ggtctcactg accgtcaggc tcagcgggag tctgaagagc agcgtcttcg tgacgtgtat
     1441 ggcgataatc cgcaggcgct ggcccgggtc accggggcac tgaaacagac atgggcggat
     1501 gaagacatgc tgcgcggtga ctggctggcc gggctgaagt ccggctgggg ggagtgggcg
     1561 gaaagtgcga cggacagttt ttcgcaggtt aaaagtgctg ccacgcagac ctttgatggt
     1621 attgcacaga atatggcggc gatgctgacc ggtgcagagg cagactggcg gggattcacc
     1681 cgttcggtgc tgtccatgat gacagaaatc ctgcttaaac aggccatggt gggcattgtc
     1741 gggcgtatcg gcagcgccat cgggggagcc attggtggtg ctggcgcatc cgcttccacg
     1801 gggacagcca ttcaggctgc ggcagcgaac tttcattttg cgaccggcgg atttacgggc
     1861 acgggcggca aatatgagcc tgcggggata gttcaccgcg gggagtttgt cttcacgaag
     1921 gaggcaacca gccggatagg tgtggggaat ctttaccg
//
LOCUS       DAZFFI010000300         1955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFFI010000300 DAZFFI010000000
VERSION     DAZFFI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.302"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1955
                     /locus_tag="ACHJ12_005210"
     CDS             <1..>1955
                     /locus_tag="ACHJ12_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4992829.1"
                     /translation="LAENARNAGELGRFMSVARVAAGGGVAVLAALAAAAWQAEQADR
                     ALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRI
                     MGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERT
                     KGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGAL
                     SLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKDVPGVSALFGELAEENREFIE
                     KTKRDELALRKSIAERDARIRQGEMGYIRRSRATGVSKGPGQQEAVSRLAEELTGKKH
                     TSPKTRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLK
                     EAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSA
                     KQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPQALAQVTGALKQTWADEDMLRGDW
                     LAGLKSGWGEWTESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSM
                     MTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGKY
                     EPAGIVHRGEFVFTKEATSRIGVGNLYR"
BASE COUNT          460 a          453 c          688 g          354 t
ORIGIN      
        1 ctggctgaaa atgcccggaa tgccggtgaa ctggggcgat ttatgtcggt ggcccgtgtg
       61 gcggcaggtg gcggggttgc cgtactggcc gcgcttgctg ctgccgcctg gcaggcagag
      121 caggctgacc gggccttgtt gcgttcactg accctgaccg gaggggcggc tgccaccaca
      181 acggcagaat tgtggaaaat ggccggggtg atcagcgatg aagccggtgg tggtatcaga
      241 caggcggcag aaaatctggc ccgtctggca gaaagcggga aatataccgc cgggcagcta
      301 cggatcatgg gggaaacctc tcagagatgg ctgcagacgg tgggggacga tgccgggaag
      361 gtggaaaaag cctttgaagg gattgcagca gatccggtga aggcgctggc ctccctgaat
      421 cagcagtata acttcctgag cgtttcccag ttacgccata ttgatgagct tgagcgcacg
      481 aaaggtaaac aggctgcggt gacggaggcg atgtccctgt ttgcagatgt catgaatgca
      541 cgtctggagc aacttgataa agcggccacg ccggtggaaa aaatctggga cgatgttaaa
      601 acctggactt ctgacgcatg ggcatggata ggtgatcata ctctgggggc actcagtctg
      661 atcactgacg tggtggccgg aaccgttgaa caggtgaagc tgctgcttgt gcagggggat
      721 ctggcgctgg ctgaatttat tcagtcagcc tgggaaacga caaaagatgt ccccggcgtc
      781 agtgcgctgt ttggcgagct ggcagaagag aaccgggaat ttattgagaa aacaaaacgc
      841 gatgagctgg cgctgagaaa atccattgcg gaacgggatg cgcgtatacg ccagggggaa
      901 atggggtata tcaggcgttc gcgtgcaaca ggcgtcagca agggaccggg gcagcaggaa
      961 gccgtcagcc gtctggctga agagctgaca ggtaaaaagc atacatcacc gaaaacgcgc
     1021 tctgccgggg agagggaaga ggagcaggca agagaggctc tgcttgccct tgaagctgag
     1081 ctcaggacgc tggagaagca cagcggtgcg aatgagaaaa tcagccggca gcgccgtgat
     1141 ttatggaagg cggaaagtca gtatgcggtc ctgaaagagg ctgccacgaa acgacagtta
     1201 tccgggcagg aaaaatccct gctggcccat gagaaagaga cgctggagta caaacgtcag
     1261 ctggctgagc tgggcgacaa ggttgaatac cagaaacggc tgaatgagct ggcacagcag
     1321 gcggtgcggt ttgaagagca gcagagcgcg aagcaggccg ccatcagcgc aaaagcccgt
     1381 ggtctcactg accgtcaggc acagcgggag tctgaagagc agcgtcttcg tgacgtgtac
     1441 ggcgataatc cgcaggcgct ggcacaggtc accggggcac tgaaacagac atgggcggat
     1501 gaagacatgc tgcgcggtga ctggctggcc gggctgaagt ccggctgggg ggagtggacg
     1561 gaaagtgcga cggacagttt ttcgcaggtt aaaagtgctg ccacgcagac ctttgacggt
     1621 attgcacaga atatggcggc gatgctgacc ggtgcagagg cagactggcg gggattcacc
     1681 cgttcggtgc tgtccatgat gacagaaatc ctgcttaaac aggccatggt gggaattgtc
     1741 gggcgtatcg gcagcgccat tggcggtgct ttcggtggtg gtgcatctgc ttcctcgggg
     1801 acggccattg aggctgcggc ggcgaacttc catttcgcga ccggaggatt tacggggacg
     1861 ggcggcaaat atgagcctgc ggggatagtt caccgcgggg agtttgtttt cacgaaagag
     1921 gcaaccagcc ggataggtgt ggggaatctt taccg
//
LOCUS       DAZFFI010000301         1950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFFI010000301 DAZFFI010000000
VERSION     DAZFFI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.303"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..810
                     /locus_tag="ACHJ12_005211"
     CDS             <1..810
                     /locus_tag="ACHJ12_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO4992830.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..>1950
                     /gene="rhsD"
                     /locus_tag="ACHJ12_005212"
     CDS             877..>1950
                     /gene="rhsD"
                     /locus_tag="ACHJ12_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO4992831.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGLWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGR"
BASE COUNT          433 a          533 c          623 g          361 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtcc ctgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc tgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaagg
//
LOCUS       DAZFFI010000302         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFFI010000302 DAZFFI010000000
VERSION     DAZFFI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.304"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..726
                     /locus_tag="ACHJ12_005213"
     CDS             <1..726
                     /locus_tag="ACHJ12_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO4992832.1"
                     /translation="VALPENFRIHDLEKFNLNRFRFRGALSTASIDDFTRYSKDLADE
                     GTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGERNSQKSL
                     AEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLMESV
                     EAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQEEMANE
                     FRDLLVEKFKDSKVETFIGTFTA"
     gene            855..1391
                     /gene="yfdR"
                     /locus_tag="ACHJ12_005214"
     CDS             855..1391
                     /gene="yfdR"
                     /locus_tag="ACHJ12_005214"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4992833.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVMPLAPGHA
                     YGMFMERFNELSELRTYA"
BASE COUNT          569 a          440 c          432 g          504 t
ORIGIN      
        1 gtggcattgc ctgagaattt ccgcattcat gatctggaaa aatttaattt aaatcgcttc
       61 cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta ttctaaagat
      121 cttgcagatg aaggcacccg ctgctttatc gatgccgata atatgcgagc cgtcagtgtg
      181 cttaacctag gtactattga tgaaccaggt cacgcagata acaccgccac tctcaaactg
      241 aaaaagacag caccgttctc tgccctgctg tctgttaatg gcgagcgtaa ctcccagaag
      301 tcactggcag aatggattga agactgggcc gactaccttg tgggctttga tgctaatggt
      361 gacaccattc aggcaaccaa agcggctgcg gcagtccgta aaatcacaat tgaagcaaac
      421 cagaccgctg attttgaaga caatgacttc agcggcaaac gctctctgat ggagtctgtc
      481 gaagcgaaaa ccaaagacat tatgccagtg gcatttgaat ttaaatgcgt tccgtttgaa
      541 ggcctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggcga tcgtcctgta
      601 ctggttctgc gcattattca gctggaagcg gtgcaggaag aaatggctaa cgaatttcgt
      661 gatctgcttg ttgagaaatt caaagacagc aaagtagaaa cctttattgg tactttcacc
      721 gcctgatttc attactgcaa atgcccctgc gggggcattt atggaaacgt aattaactca
      781 ataatcaccg gatggtgaga gcttcctttt agcagaattc aacgcggtgc agcgcatata
      841 aagtggagaa cgaaatgtca tttattaaaa ctttttccgg gaagcatttt tattatgaca
      901 ggataaataa agacgacatc gtgattaacg atatcgcagt ttccctttca aatatctgtc
      961 gctttgcagg acatctttca cacttctaca gtgtcgccca gcatgcggtg ctttgcagcc
     1021 agctggtgcc gcaggaattt gcttttgaag cgttaatgca tgatgcaaca gaagcgtatt
     1081 gccaggatat tcccgctcca ctgaaacgcc ttcttcctga ctataaacgg atggaagaaa
     1141 aaatagacgc cgtaatccgt gagaaatacg ggttaccccc ggttatgagc acgcctgtga
     1201 aatatgccga tctcatcatg ctggcaaccg aacgccgcga tctcgggctt gatgatggct
     1261 ctttctggcc agtactggaa ggtatcccgg caacagagat gttcaacgtg atgccactgg
     1321 caccgggcca tgcctacggg atgtttatgg aacgctttaa cgagttatcg gagttacgca
     1381 catacgcatg agtctttcaa ctgaacaacc gctggagggg tagcaatgag caacttaatg
     1441 acaacaaaac aagtcgccga attctgtggc gtttcaatat cgacggtgct tcgctggaac
     1501 agcgtaaaca ggaggacagg ccagaaatac aggccagatt ttccagatcc tgatattaaa
     1561 tcctgcccaa ataaatgggc atcacgcaaa atatacagat ttgctggagt tattgagtaa
     1621 tgagagttag ccaagaggtg agatagtatc catctatggc acagaactaa acataatctg
     1681 actatatgct ctgtgccaaa aacagatgtt atatcatgta ttgtatgctc atcctgcaaa
     1741 atataaattc cgcagacacc cctcacctct gaatattaat ccacaatgga tgcgacaaaa
     1801 aagattttga gttggtatca ggcgaaatat taataattat aataccccat gcacaatgcc
     1861 atctgagtca tcagatcctg gtctctatca atatttatct tgccatcaga aaaatccaac
     1921 acaaagaaat gatgaaattt cggcg
//
LOCUS       DAZFFI010000303         1914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFFI010000303 DAZFFI010000000
VERSION     DAZFFI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.305"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1914)
                     /locus_tag="ACHJ12_005215"
     CDS             complement(<1..>1914)
                     /locus_tag="ACHJ12_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4992834.1"
                     /translation="TLNTGDGTVILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQI
                     EGDRIRWGYRGGTLDINGNDMAFNRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMW
                     HGHFTGNLSVLNNTSSAVDFIMDGGADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATA
                     LRKQGDNSVLTQPVSFAQKDWESRTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMV
                     LGSDSLYLDMKDGTGSSSAPVKGTSAVGGASGSSTFRGNVNMRHSALTVRDHFTGSIT
                     ASDSRIAVSSENVRLEGNSRLTSSALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGG
                     SVRNDVYERLLAWSEERGGLNGSGEYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTG
                     NSSVKHLESSGSALYFSRPGGEFHTLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRG
                     KNNLLLVDFTERSDGQKALNIPLVTAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTG
                     GTAWQLNVVQPETAAEPVATRQDAETPNPVEAVSPLPSPVSAPSSAPEASVTDVLTGE
                     NAGESGEYRDETRWLLTGYRSTVNSSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRD
                     INGEAGAWARIMSG"
BASE COUNT          409 a          651 c          436 g          418 t
ORIGIN      
        1 ccgctcatga tacgggccca tgcaccggcc tcgccgttga tgtcccgcag gtcacccata
       61 cgtttgttca ggttgttgac ctcattaatg aagttacggt gccccattga catcagcatc
      121 cccgcatccc tgaccgcgga gctattaacc gtactccggt atcctgtcag cagccaccgg
      181 gtctcatcac ggtactcacc tgattccccc gcattctctc cggtcagtac atccgtcact
      241 gacgcctccg gagccgatga cggagcagaa accggtgaag ggagcggtga tacagcttcc
      301 acaggattcg gcgtctctgc atcctgccgc gtcgccacag gctctgcggc agtttccggc
      361 tgcacaacat tcagctgcca ggccgtgccg cccgtcccct gttcagccct gacaacaggt
      421 gtgatatgac tgaagccggt atcacgcgtt ttcacactga acacatccgc accggtaccg
      481 gccggcgccg tcaccagagg gatattcagc gccttctgcc cgtcagaacg ctcagtaaaa
      541 tcaaccaaca acaggttatt cttaccccgc agcgattctg tcacccgaag ctggtctgag
      601 tggttaaggt ccgtccgcat caccagaaca ctgtcgctga tatccatact gcctgccgtc
      661 agggtatgga actccccgcc cggcctgctg aaatacagag cacttcccga tgactcaaga
      721 tgcttcacgg atgagtttcc cgtcatcatc caggccgcat tctgcagatt cacattacct
      781 gcactccctc tgacataact ccgcaggaga ccagctgccc ccgtcataaa atcatactca
      841 ccgcttccgt tcagcccgcc acgctcctct gaccaggcca gcagacgttc atatacatca
      901 ttcctgacac ttcccccgtc caccaaaagg gttcctctgt ccagtgtcac accaccatct
      961 gtctccagtc cccccttcac atgtaaacgc ccgccgtcac tgacagtaag tgcagaagag
     1021 gtcaggcggg aattcccttc cagacgcaca ttctctgaac tcacagctat ccggctgtcc
     1081 gatgccgtta tactgccagt aaaatggtcg cggaccgtca gggcagaatg acgcatattc
     1141 acattcccgc gaaatgtact gctaccggat gcaccgccca cggcagacgt ccccttaacc
     1201 ggcgcactgc tgctccccgt gccgtccttc atatccagat atagcgagtc acttcccagc
     1261 accatggtgg cattatcagc atcaatatct cccgtcagtg tcgcattgcg ggaaaggctg
     1321 aatgccgccc ccttcagttt cagttgaccg atgctgaacg ttctgctctc ccagtccttc
     1381 tgagcaaatg ataccggctg cgtcagaaca gaattatcac cctgctttcg caatgcagtc
     1441 gcaaccgcct cactgctgac agcatgaaca accggatgcc cctggatata cagtcccccg
     1501 ccctgctgtg taaaactgcc ggacatatcc gctccgccat ccatgataaa atccaccgca
     1561 gatgacgtgt tgttcaggac gctgaggtta ccggtaaaat gaccatgcca catcacggcc
     1621 ttctgccctg actggctgaa atccagacgg actgtggcag gacgcgcccg gcttgtgagt
     1681 actgcccctt catcagccgc agccaggcgg ttgaaggcca tgtcatttcc gttgatatca
     1741 agcgtgcctc cccggtatcc ccagcgaatg cggtcccctt caatctgatg actgtcctgc
     1801 agcaccacaa ctggtcgtcc gctgacaata ctgacttcac tgaatgcccg gaccctgcct
     1861 gcagcatcag ccttctgtgc cagaatgacc gttccgtccc ccgtattcag ggtg
//
LOCUS       DAZFFI010000304         1846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFFI010000304 DAZFFI010000000
VERSION     DAZFFI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.306"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1846)
                     /gene="espI"
                     /locus_tag="ACHJ12_005216"
     CDS             complement(<1..>1846)
                     /gene="espI"
                     /locus_tag="ACHJ12_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFO4992835.1"
                     /translation="RLMTVSLVLSVTLSALPGKASTVSAEIPYQTFRDFAENKGVFTP
                     GVTGIEINDNNGNKVGVLDVPMLDFSSLSRDGHTTLIHPGYVVSAKHGGLQSVSSATF
                     GYDQIYKIVDNNLAGIDFSAPRLNKLVTEVIPADIQGKDKFNNNRYTAFYRAGVGSQY
                     IRYANGTDKLLQAYTPEKAYLTGGTVGKPYYTHYNGMKMISANPGNTFDKNQGPLASY
                     GQSGDSGSPLYAWDNIDKKWVLAGVTLHNYGVKGARNDWLLIPHDFISQKLQDDLKPI
                     IVASPEENILRWEFDRSRGTGTLSQGEKIFSMTGSVNGNANTGNNLVFSGNEGKIELV
                     SSVEQGAGYLQFDKDYTVLTNNNSTWTGAGIIVGDEANVKWGVNGIAGDNLHKVGSGT
                     LTVNGHGENKGGLKVGDGVVVLEQQPDANQKQQAFSHINIASGRATVKLNGANQVDAD
                     NISWGYRGGKLDLNGYDFTFSRLQAADYGAEISNDNQTDKSIVTLSLSPLKAEEINVV
                     VNNINIMGGTGKPGDLYYTTFDGNYYLLKSNRYGSALFGALNNQSEWQRLGKDKEKAI
                     GLYTQMKMQESAPLSYIYHGKITGNTSVEIPKLAGNDILTLDGSVSISG"
BASE COUNT          510 a          441 c          321 g          574 t
ORIGIN      
        1 cctgatatac tgacagagcc atcaagcgtt aaaatatcat tgcctgccag tttggggatt
       61 tccacactgg tattaccggt tatttttcca tgatatatat atgataaagg agcgctttcc
      121 tgcattttca tctgagtata taacccaatt gctttttctt tatccttacc cagcctttgc
      181 cattcgctct gattattcag cgcgccaaac aaagcgctgc catatcggtt acttttcaac
      241 agataataat ttccgtcaaa ggtcgtataa tacagatcac ctggtttgcc tgtccccccc
      301 attatattta tattattaac aaccacattt atttcttctg ctttcagagg agataacgaa
      361 agtgtgacta tggatttatc tgtctgatta tcgttgctga tttcagcacc ataatctgca
      421 gcctgaagac gggaaaaggt aaaatcatac ccatttaaat ccagtttccc accccgatat
      481 ccccagctga tattatctgc atctacctgg tttgccccgt taagtttaac tgttgcccga
      541 ccactggcta tattgatatg gctaaacgcc tgttgtttct ggtttgcatc tggctgttgc
      601 tcaagaacaa caacgccgtc cccaacttta aggccacctt tattctcacc atgaccatta
      661 acagttaatg tcccggaacc aactttatgc agattatcac cggcaatgcc attaactccc
      721 catttgacat ttgcctcgtc accgacaata attccggcac cagtccatgt actgttatta
      781 tttgtcagta cagtgtagtc tttatcaaac tgaagatatc cggctccctg ctccacactg
      841 gataccagtt cgattttccc ttcattacct gagaacacaa gattgttccc ggtatttgca
      901 tttccgttta cactaccagt catggaaaaa atcttctctc cctgactgag agtacctgta
      961 cctctcgaac gatcaaattc ccagcgtaag atattctcct ccggagaagc aacaataatt
     1021 ggtttaaggt catcctgtaa tttttgactg ataaagtcat gaggtatcag gagccagtca
     1081 tttcgtgcac cttttactcc ataattatgc agagtaactc cagctaatac ccattttttg
     1141 tcaatgttat cccaggcata taacggcgaa ccactgtctc cactctgtcc ataactggca
     1201 agaggtccct ggtttttatc aaaggtattt cccgggtttg ctgaaatcat cttcataccg
     1261 ttatagtgag tataataagg tttccccact gttccgccgg tcagataagc cttttctgga
     1321 gtgtaagcct gcagtagttt atctgtgcca tttgcataac ggatatattg agagccaacg
     1381 cccgcacggt aaaaagccgt ataccggtta ttattaaatt tatcctttcc ctgtatatct
     1441 gcgggaatta cttctgtaac aagcttattt aatcgtgggg cagaaaaatc tataccagca
     1501 aggttattat caactatttt atatatctgg tcataaccaa aagttgctga tgaaacactt
     1561 tgtaaaccac catgtttagc cgatacaaca taaccaggat gaatcaatgt ggtatgacca
     1621 tcacgagaaa gactggaaaa atcaagcatg ggaacatcaa gaaccccaac tttatttcca
     1681 ttgttgtcgt ttatttcaat tcctgtcact ccgggggtaa acacaccttt attttcagca
     1741 aagtcacgaa aagtctgata tggtatttct gcgctaaccg ttgatgcttt acccggtaaa
     1801 gcagaaagag tcactgataa taccagggaa accgtcatta atcttc
//
LOCUS       DAZFFI010000305         1844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFFI010000305 DAZFFI010000000
VERSION     DAZFFI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.307"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1844
                     /locus_tag="ACHJ12_005217"
     CDS             <1..>1844
                     /locus_tag="ACHJ12_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4992836.1"
                     /translation="GTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTK
                     EGLYTGESIAIGAKTKATKEQSIAIGGDTIASGWGAIVIGGDDLTPLKGRKYGEITIP
                     NNYDASEAKGSGSIVVGSQSKAEKDLSLAVGSVSKATGMGANAIGATSVASGEWSNAV
                     GFNAKAEAGNSIALGSSSKTGGNASNSVAIGTSASANKKSAIAIGEKSKVSFGDNNVA
                     LGSNSVDKTATNIKNATIDTKTYGDFAGSTPTGVVSVGDAGKERQIVNVGAGAVSEKS
                     TDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDGD
                     IAVTVNDKGMTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAG
                     DKLEISAETASLPVNGGNGKITVSNGDDDKLVTAKNIAEAINQSGWKIKADKDTGGVI
                     EGNAQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGNV
                     TLSSEGNKLTLSGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLKTA
                     IDGVSSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGD"
BASE COUNT          605 a          306 c          495 g          438 t
ORIGIN      
        1 ggtacaacga aatttacagc aaaagatgaa tcaaagggaa ttaatttcga ccataagtcc
       61 cctgttgcag aagggcgtaa ctctatcgcg attggtacca gtgcagaggc tggaggtgac
      121 attagcacga aggaaggttt gtacacaggc gagtctattg cgataggagc gaaaacgaaa
      181 gcaactaaag aacagtcaat tgcaataggt ggcgatacta tagcaagcgg atggggggct
      241 attgttattg gtggcgatga tttaacccca ttaaaaggac gaaaatatgg agagatcaca
      301 atccctaata attatgatgc atcagaggct aaaggcagcg gaagtattgt tgttggttca
      361 caaagtaaag cagaaaagga tttatctctt gctgtaggtt cggtaagtaa agcaactggt
      421 atgggagcta atgctattgg tgcaacatcc gtcgcatcgg gggaatggtc aaatgctgtt
      481 ggctttaatg ccaaagcaga ggctggcaac agcattgctt taggctccag ctctaaaact
      541 ggagggaatg catctaattc tgtcgctata ggaacatcag caagtgcaaa taaaaagtct
      601 gcgatagcga taggggaaaa atcaaaagtc tcttttggtg ataacaatgt tgctcttggt
      661 agtaattctg ttgataagac cgcaacaaat atcaaaaatg ccactataga tacaaaaacc
      721 tatggggatt ttgcgggttc tacgcccact ggtgttgtat ctgttggtga tgcgggtaaa
      781 gagcgtcaga ttgttaacgt gggggcgggt gctgtatctg agaaaagtac agatgccatt
      841 aacggtagtc agttatatgc gatagcgaag acactgaaag atgaccttga tgccattaat
      901 ccgacgtgga cgctaaagga tgataaggga ggttctgcga ctattaatgg tgggaaggat
      961 aataagaatg gtaacacttt aactgttaaa ggtgatggag atattgcagt tactgttaat
     1021 gataaaggaa tgacgattga cggaacaacg ctgaaaaatg aaataaaaaa tgatgccatc
     1081 aaaattaaag gtggagatgg taagacgctg acgaagagtc ttggtgacaa tgtcgaaatc
     1141 actggcaaag gcccaataaa aacccaggtg gctggagata aactggagat ttcagcagag
     1201 acagcttcgt tgcctgtaaa tggtggtaac gggaagatta cagtaagcaa tggtgatgac
     1261 gataagcttg ttactgcgaa aaacattgct gaagccataa accagtctgg ctggaaaatt
     1321 aaagcggata aagataccgg tggtgttatc gagggaaatg ctcagggaac agaaacactg
     1381 ataaaacccg gtgatatggt tacgctgaaa gccggtaaaa atctcagcgt gaaacaaagt
     1441 aacggtgatt ttacattctc tgttattgat actccgtcat tcagcagtgt tcagctgaac
     1501 ggtaatggaa aaaacggaaa tgtaacgctc tcatccgaag gtaataagct gacattgtca
     1561 ggcggtaatg gtgccggaca aaatggtgtt gttatcagtg gtgtggcaag tggactggaa
     1621 ggtaaggaac tttcgacgtt aacgaatact gataaagaga tgaaaaatgc cgccagtgtt
     1681 gcagacctga aaacggcaat tgatggtgtg tcctccagta tgcagggtgc aggtttcgca
     1741 ctgaaagact ctgatgataa ggaagtgaaa cagtctcttg ggcaggcgat taaagtatcc
     1801 ggggacagca atatcaccac cacagccatc accaatgggg ataa
//
LOCUS       DAZFFI010000306         1824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFFI010000306 DAZFFI010000000
VERSION     DAZFFI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.308"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..440
                     /locus_tag="ACHJ12_005218"
     CDS             <1..440
                     /locus_tag="ACHJ12_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4992837.1"
                     /translation="LISAGCSTLDSVGNKAQPEHKDQIMVYALMHSISRSQNVNHHEL
                     IITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLSKARISSIDFV
                     TPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ"
     gene            484..1398
                     /locus_tag="ACHJ12_005219"
     CDS             484..1398
                     /locus_tag="ACHJ12_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992838.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            1408..>1824
                     /gene="ykfM"
                     /locus_tag="ACHJ12_005220"
     CDS             1408..>1824
                     /gene="ykfM"
                     /locus_tag="ACHJ12_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFO4992839.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNVHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYI"
BASE COUNT          585 a          333 c          339 g          567 t
ORIGIN      
        1 ggctaatttc agcaggttgc tcaacgcttg attctgtcgg aaacaaagcg cagccagaac
       61 ataaagatca gatcatggtt tatgcgttaa tgcattccat ctcaagaagt caaaatgtca
      121 atcatcatga attaattatc accaaaccgg ttgataaatc ctcaccattg ctggcaaaag
      181 cacttagtga caatgaaaaa atggcaatat gtgaatttat actctaccga accagtaaag
      241 cgggtattta ccagccttat tataaaataa atctgtcaaa agcccgaata tcaagtattg
      301 attttgtcac accgcatgct gtcctggaaa aagaactgga accacaggag agaattgcct
      361 ttatttatga agatatttcc tgggagcata cgttagccgg tacgaatgca atgagtaaat
      421 ggcaggatcg tgtacagtaa cttactgtaa taacataata aattactatt gcggagagat
      481 aaaatggctg gtttaaggaa taacaacaac acacagaatg cacagtgggc tgattatgtg
      541 ggtgacattc ttcggggggc tcagccgata aaccagctag tacctcagca cccttatctt
      601 aacgacgtcc cactcattga tgagttaagg catcagaaca ctcatcatgt tattctcttg
      661 acacttgatg ttgcaaaaaa aatactcagc ccaataacat catttgatta cattcatttt
      721 atcactacac atccttcagg tataaaagat acactcgcct ggttagttaa cgcgagtaaa
      781 ttaatgactg agtttgatga caatgggaaa attatattta accttaatgc cttaaagtat
      841 acaaaagcaa gttactttga aatacttgga gagaaatata taaaaatcac aacatccagc
      901 ccctggcttt tagaaaagct cggtaaatat attttctctt cacgagcgcc tcaagttctg
      961 gaactcgcta ttggctggcg aggagcttta tctgaatcaa taaaaggagt taagttctgt
     1021 atctggtttt cagtagcctg gagaaccata gaatttatta tgagttcaga gcgagatctg
     1081 gttaattttc ttggcgattt ttcaatggat gtggccaaag cagttatagc cggtggtgtt
     1141 gcaaccgcta ttggaagcct ggcttctttt gcctgtgtta gctttggctt tccagtaatt
     1201 cttgtcggag gagcaatttt actgacaggg atagtgtgta cagttgtttt aaatgaaatc
     1261 gatgctcaat gccatttatc agaaaaatta aaatatgcaa ttagagatgg actaaaacgg
     1321 caacaggaac ttgataaatg gaaaagggaa aacatgactc catttatgta tgttcttaac
     1381 actccacccg tgatatgagg attaaccatg cgtttacatg ttaaattaaa agagtttctt
     1441 tcgatgtttt ttatggccat attatttttc cctgcattca atgcttcttt gttttttacc
     1501 ggagttaaac ctctatattc aataataaaa tgctccacgg aaatatttta tgattggaga
     1561 atgttgattc tgtgctttgg tttcatgtcg ttttcctttt taaatgttca tgtaatttta
     1621 cttacgataa taaaatcatt cttaatcaaa aaaacgaagg tagtcaattt cgccacagac
     1681 ataacaatac agctaactct tatattttta ctaatagcta tcgtaatagc acctttaata
     1741 gctccttttg tcacaggata tgtcaatgct aattatcacc cctgcggtaa taatacggga
     1801 attttcccag gagcaatcta tatt
//
LOCUS       DAZFFI010000307         1823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFFI010000307 DAZFFI010000000
VERSION     DAZFFI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.309"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..367)
                     /locus_tag="ACHJ12_005221"
     CDS             complement(44..367)
                     /locus_tag="ACHJ12_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992840.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(400..1302)
                     /locus_tag="ACHJ12_005222"
     CDS             complement(400..1302)
                     /locus_tag="ACHJ12_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO4992841.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRNNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGRDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
BASE COUNT          504 a          450 c          318 g          551 t
ORIGIN      
        1 tataagcctg tatattttta aatcacaaaa gatgaatgtc aagttattca acatcaatcc
       61 aaagaggaat attatgtatt cccttaacgg gttctcccag aggaaaaaca caggtccctt
      121 taatcagtat ttttcttccc gaccatctga catcacagtt aaaatctccc accacatcaa
      181 aataatccgc aatggattca tagatgtccc attcatcata gatatcccat ttatctgctg
      241 ttccttcaca ttcaaactcg gcctgatatc cggaatcaac atatcgcgcc agatagtgtg
      301 accacaaagg cgaatttttt gtaatctctt ttacagagat tattttcagc aaaatagtac
      361 aatccataat atttacccgt acgaaatcgc catgagttat tactcatagc ctaacaactg
      421 acaataaacc tgttcaatat atcgtcctct gccatcacca tgaccaagat caattgatgt
      481 cagtgcaagt gcctccttat aactgtaacc gttcataata tgatatttca tggcacttgc
      541 agcgtaactg taccgaaaac tgtgtgcagt ctcatctctg cctttcagtt ctccataacg
      601 acgtaatgta ttaagataat aatccattgc cgacttaaga ttatctctgt ctataagacg
      661 tccattatgt tcactggcat atttgagtgc ataattaaca gcatcaagaa cgttatcccg
      721 gtcaacaacc gttgtcattc tttcccgtcc ccctttcgta ccaaaaatca ctcttatttt
      781 ttcatctccc cgttgtaacg cttttttcca ggttcgtaat gatttggccg actggatagc
      841 ttcttcattt ctcagaccga ggtaacgtga cagtttaaca acagcagcca cccctttatc
      901 ttttgcttcc acacgctgaa gaatctcccg aaaacgctca tccgtgatta atttttttgt
      961 tcccttacga ctggagccgg aaatgcctaa cgctttattg ctgagacgag gatgttcggg
     1021 atcagccatc ttatatttac ctgcctgacg taacataacc cgtattgcag acatttcatt
     1081 ttcccttgtt cgcccagaaa cattattccg gcttctcata tatagctcaa tatgccggac
     1141 ttttacctgc gcggcagaac tgatctgtac attcattttt gccagacttt cagcaaagcg
     1201 gtgcatgatc ctggtgtaat cactcagctt cttgaagctg cctttgtctt ttcgaatgtg
     1261 agcaaccaga tcctgtttca gtaatttttc acgtctggac atcaacttct cctgcacata
     1321 ataattttta ccttaaacag ctgtacgttc ggcagatgag tttttctgtt gtgccgtgaa
     1381 cacaacagaa aaactcatct gcatttctcc ctgacgacac ggttaaacgt tgcgatcctt
     1441 cgggtagtga ctgcctgtgc gccagggtag cgcagattgt ttaggtattg aggggtaaca
     1501 aatcggtgtt gcacaccgac tgcagcattt gctgtacctg tctccaggtc gcccccgaca
     1561 ccacctctaa taagtggtat cgccgattcg gcatctcact acataaaact ccttaaaaaa
     1621 acaattgatt ctgcaaaaaa ccatttatgg acgttattca caaaatcgtc cgtaaatttg
     1681 cagaagaggt tgaaaaaaca ctctcacgcc gggtcacttc ttcaaatgtt aaaaaacatc
     1741 acgtgaaacg gcagttcgtg cgggttcgtt aagcagacgc gcgtgcgtca ctttgtcatc
     1801 gtgacacgct gacgaaagtg acg
//
LOCUS       DAZFFI010000308         1818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFFI010000308 DAZFFI010000000
VERSION     DAZFFI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.310"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..473
                     /locus_tag="ACHJ12_005223"
     CDS             <1..473
                     /locus_tag="ACHJ12_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992842.1"
                     /translation="LPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLP
                     DLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLGSFTGTTIL
                     KQITPLSPAIDFDNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            473..1075
                     /locus_tag="ACHJ12_005224"
     CDS             473..1075
                     /locus_tag="ACHJ12_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4992843.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAVGVGI
                     PAYSCLDAPGTYKAGYAICRSADFNSWEYVPDHRGEIVYNTETGDAKEITTPGDYPEK
                     TTTIAPLTPYDKWDGEKWVTDTETQHSAAVDAAEAQRQSLIDTAMASISLIQLKLQAG
                     RKLMQAETTRLNTVLDYIDAVTATDISTAPDVIWPVLPEA"
     gene            complement(1047..1478)
                     /locus_tag="ACHJ12_005225"
     CDS             complement(1047..1478)
                     /locus_tag="ACHJ12_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4992844.1"
                     /translation="MEKTYYFNPSDSGFYISPDSQTIPENATKINFTIYSEFAGVAWP
                     DGKILGSDKNGFPAWQDAPPLTSEELISIAESNKQRLVNQANEYMNSKQWPGKAAIGR
                     LKGEELAQYNLWLDYLDALEMVDTSSAPDIEWPTPPAVQAR"
     gene            complement(1481..>1818)
                     /locus_tag="ACHJ12_005226"
     CDS             complement(1481..>1818)
                     /locus_tag="ACHJ12_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992845.1"
                     /translation="RNVGTGENQVPDMNSFGNSLTANGYQKLPGGMIIQWGSFSVSPT
                     GGSVGTVDITLPVAFPAACRSVNALISTNDPSARSVGFDIGSTNRTKIRFTYTSATTN
                     SIYWMAVGY"
BASE COUNT          505 a          426 c          412 g          475 t
ORIGIN      
        1 cattacccgt tggtgtgcct gttccgtggc cttcagccac tccgccaaca ggctggctga
       61 aatgcaacgg agcagctttt tctgctgaag aatacccgga actggcaaag gcttatccga
      121 caaataaatt acctgattta cgcggtgagt ttattcgtgg ctgggatgac ggacgtggag
      181 tggataacgg aaggggatta ttaacgcttc aggacggtgc gattgtcagt cataaccact
      241 attggggaat ctggacttca cgaactaacg accagactct gggaagtttt acaggcacca
      301 cgattttaaa acaaatcacg cccctgtctc cagccattga cttcgataat tacccaattc
      361 ccaacccggc tattacagag ggtggtgttg ttgcggcaac gactaaacct gcaggtgcga
      421 atgaaacacg cccacgaaat gtcgctttta actatattgt gagggctgca taatgaataa
      481 cgcagaatta aacagtgaat taattgccac tatggcagga gaaattactg tttataactt
      541 tgacgtcatg agtcgggagt atatttcagc ttcaactgaa tatcttgctg ttggtgtcgg
      601 cattccggca tattcctgtt tagatgctcc aggcacctac aaagctggtt atgcaatctg
      661 ccgctctgca gattttaact catgggaata tgtaccagac catcgcggtg aaatcgtcta
      721 taacaccgaa acgggagacg ccaaagaaat cacaactccg ggtgattacc ccgaaaaaac
      781 aaccactatc gccccgttaa cgccatacga taaatgggat ggtgagaaat gggtgacgga
      841 tactgagaca cagcatagcg ccgcagtaga cgcggcagaa gcacaacgtc agtcactgat
      901 tgatactgca atggcttcca ttagtctgat tcaactgaaa ttgcaggccg ggcggaagct
      961 gatgcaggca gagaccaccc gacttaacac tgtgctggat tacattgacg cggtgacggc
     1021 aacagatatc agcaccgcgc cggatgtcat ctggcctgta ctgccggagg cgtaggccat
     1081 tcaatatctg gcgcactgga agtatcgacc atttccagtg cgtccaggta atccagccac
     1141 aaattatatt gcgccagttc ctcacctttc agacgaccaa tcgccgcttt accaggccat
     1201 tgtttgctgt tcatgtattc gttggcctga ttaactaatc gctgtttatt tgattcagcg
     1261 attgagatta attcttcgct ggtaagtggt ggcgcatcct gccatgctgg aaaaccattt
     1321 ttatctgaac caagtatttt accatcaggc catgcaactc ctgcgaactc agaataaata
     1381 gtaaagttta ttttcgtagc gttctcagga atggtttggc tatctggtga aatataaaat
     1441 cccgaatcag agggattaaa ataatatgtc ttttccatag ttaatacccc acagccatcc
     1501 agtaaattga atttgttgta gcagaagtgt aagtaaatct gattttagtt ctgttggtac
     1561 ttccaatatc aaagcccaca gaacgggcag aaggatcatt agttgaaata agagcgttta
     1621 ccgaacgaca agcagcaggg aaagctacag gtaatgttat atcaactgtt ccaacacttc
     1681 ctccggttgg tgagacagaa aaactccccc attgaataat catcccccca ggtaatttct
     1741 ggtatccatt ggcggtcaat gagttaccaa agctgttcat atccggtacc tgattttctc
     1801 ctgtccccac attcctct
//
LOCUS       DAZFFI010000309         1814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFFI010000309 DAZFFI010000000
VERSION     DAZFFI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.311"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..658)
                     /locus_tag="ACHJ12_005227"
     CDS             complement(<1..658)
                     /locus_tag="ACHJ12_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992846.1"
                     /translation="MGRDITLYPKKATRNELKNYLENLGFKRCKHLWEWPQGTLNYSW
                     FDDTDFKSTDGVSADIYPVSNDELHISGNKWALHVRNLYSASWHDVKMLNDVLKGARS
                     LFGGTLIGDYGKNRYAPLWKDSSSPISRGISSIFNHVHHEISAVKHALPEPSIKLNLP
                     EDGGLSEYFDYMQCMDPSRVIYNGLVPFAVAMFEYFFSRAFQILIKYDPFAIAKRTSY
                     K"
     gene            850..1110
                     /locus_tag="ACHJ12_005228"
     CDS             850..1110
                     /locus_tag="ACHJ12_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4992847.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            1301..1441
                     /locus_tag="ACHJ12_005229"
     CDS             1301..1441
                     /locus_tag="ACHJ12_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992848.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            1703..>1814
                     /locus_tag="ACHJ12_005230"
     CDS             1703..>1814
                     /locus_tag="ACHJ12_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO4992849.1"
                     /translation="MALIKCPECQKEVSDSALYCPACGKQIKKLKRSFFGK"
BASE COUNT          553 a          386 c          367 g          508 t
ORIGIN      
        1 gcttatacga tgttcttttt gctattgcga aaggatcgta ctttataagt atttgaaaag
       61 ctctagagaa aaagtattca aacatagcaa ccgcaaaagg tactaatcca ttatatataa
      121 ctctactggg atccatacat tgcatgtaat caaagtattc ggataagccg ccatcttccg
      181 gtaggtttaa tttaatagaa ggttcaggta aggcatgttt aacagcggaa atctcatgat
      241 ggacatgatt aaatattgaa gaaatgccac gactgatcgg actactagaa tccttccata
      301 gaggagcgta cctatttttc ccataatcac ctataagtgt tccaccaaaa agactccttg
      361 cgccttttag aacatcatta agcattttta catcatgcca actagcgcta tataaattac
      421 gcacatgcaa tgcccattta ttaccagata tgtgtaattc gtcatttgaa acaggatata
      481 tatcagccga aactccatct gtagatttga agtccgtatc atcaaaccaa gagtaattca
      541 acgttccttg gggccattcc cacaaatgtt tacacctttt aaaacctaaa ttttcaaggt
      601 aatttttaag ttcgtttcta gttgcttttt tagggtacag cgttatatct cttcccatgc
      661 tttattcacc tgatatacaa tattttaaat tttaactatt atgaacgtat gaattttcac
      721 attggatgta tggaagttca acaactattg aaagttcaat tttcactctt cgcacagagc
      781 gagctgttag ttggaattag ggatcgtaca atctagaata gcggcaacac cacgttaagg
      841 gaggtcgcta tgttccgttg tccgctttgt ggcgcatctg cccgtatccg cacaagtcgt
      901 ccggaaaatg attcaaacac cgtgcggcaa aagtattacc agtgtaacaa tctggaatgc
      961 ggcgtatgct tctcaacact ggaagctttc cataaattca catcgaaaca cgcctccggc
     1021 gttcactctt cagaaggtat cccgtggcat gagctaccag cttcacacag gggaaacaat
     1081 cagatgagtt tgcctttacc tcagaattaa caagcagaat tgccggagta acaaaaaagc
     1141 gatagattac gcgcgggtgc ctttcggctg atggtcggag ggaatacccg aaggccagat
     1201 gtggaaaggc cccggaaaac acttttgttt aaccgaggcc ctaaccgtct accctaagca
     1261 agtgaaaggt tagcgcctct ccggaaaagg agcaagtgct atgtcgcaaa aatcgcttac
     1321 agccatcacg ttctgcgtga cggtaatcct catcatctgg atgttgcacg gttcgctgtg
     1381 cgaaatacgg atgagtttct ggggagcgga gtttgcggcg ttcttacagt gtaagcagta
     1441 aggaaaccgc gacgggggag taatcccccg tcaatcggtt gccagggtaa ggtcgataag
     1501 gcaccctatc tcacggctct aaatgcaaaa attccatgaa actgtgggat ttttgcatca
     1561 ctactgatac agatctgccc ttctatatgt ctcactctat accagctaat caattgacac
     1621 ttatcaataa aaaaaaatct gataaaatca aaatgttttg taacaaatat caactcgtat
     1681 tgatccaaag gaggcggatt acatggcatt aatcaaatgt cctgaatgcc agaaagaggt
     1741 gagcgattca gcattgtatt gccctgcttg cggtaaacaa ataaaaaaac ttaagcgttc
     1801 attttttgga aagc
//
LOCUS       DAZFFI010000310         1797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFFI010000310 DAZFFI010000000
VERSION     DAZFFI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.312"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..525
                     /locus_tag="ACHJ12_005231"
     CDS             <1..525
                     /locus_tag="ACHJ12_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992850.1"
                     /translation="YALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAV
                     LSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHNFAYNNTSAYAET
                     PGTGGGMHATNTNKTASNRVLSAGAHTHTVAIGAHTHKLAIGSHTHTITIAAYGNAEN
                     TVKNIAFNYIVRLA"
     gene            525..1124
                     /locus_tag="ACHJ12_005232"
     CDS             525..1124
                     /locus_tag="ACHJ12_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4992851.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAIFNSDKTSWHLVEDHRGKTVYDVASGGALFISELGPLPENVTWLSP
                     EGEFQKWNGTTWVKDAEAEKVFRIREAEETKNSLMQVASEHISPLQDAVDLNIATEEE
                     TSLLVAWKKYRVLLNRVDTSRAPDIEWPASPVESRSKQA"
     gene            1219..>1797
                     /locus_tag="ACHJ12_005233"
     CDS             1219..>1797
                     /locus_tag="ACHJ12_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992852.1"
                     /translation="MNVISSLKQLNRQRNIKTKKIKTKARLAFLKMRYRRKTSLQPDS
                     YKTVCIFMHMQAIGDGIVTSGFIKQLQKSGMVVYVIAPSRVSFLFTDIVGVDAFISYE
                     KNKFNELKAKIKKLNVDLVVDFSNFDNTAITRLQTLHLLRPKHSICFNHPAVTIFDTN
                     IIDNRSIHTSERMKKVLSLLKIKNNNYAAALNF"
BASE COUNT          585 a          375 c          377 g          460 t
ORIGIN      
        1 tatgccctga tgcaggggca gacttttgac aaatctgcat acccgaaact tgcagcggct
       61 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa acctgccagt
      121 ggtcgagccg tattgtctca ggaacaggac ggcattaaat cgcacaccca cagcgccagc
      181 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg aactaaatcc
      241 acaaataaca ccggagcaca tacacacaac tttgcttaca acaatacttc agcatacgca
      301 gaaactcccg gtaccggtgg tggaatgcat gcaactaaca caaacaagac agcaagtaat
      361 cgcgtattaa gcgcaggagc tcatactcac acagtagcaa taggtgcgca tacacataag
      421 ctcgctattg gttcgcacac acacaccatt accattgccg cttatggcaa tgcggaaaac
      481 accgtaaaaa acatcgcatt taactatatt gtgaggcttg cataatggca ttcagaatga
      541 gtgaacaacc acgaaccata aaaatttata atctgctggc cggaaccaat gaatttattg
      601 gtgaaggtga tgcatacatt ccacctcata caggtctacc tgcaaatagt actgatattg
      661 ctccgccaga tattccagct ggcttcgtgg ctattttcaa cagtgataag acgtcctggc
      721 atctcgttga agatcatcgt ggtaaaacgg tttatgacgt ggcatcaggg ggcgcgttat
      781 ttatttctga acttggtcca ttaccggaaa atgtcacctg gttatccccg gaaggggagt
      841 ttcagaagtg gaacggcaca acgtgggtga aagatgcaga agcagaaaaa gtgttccgga
      901 tacgggaggc ggaagaaaca aaaaacagcc tcatgcaggt agccagtgag catatttcgc
      961 cacttcaaga tgccgtagat ttgaatattg caacagagga agaaacatcg ttactggtgg
     1021 catggaagaa gtatcgagtg ctgctgaacc gtgttgatac atcaagagca ccggatattg
     1081 agtggccagc ttccccagtg gaatcaagga gtaaacaagc ataaaggctt tcatttcaaa
     1141 ccattgtagt ccagtttttt gtatagtacc ctgctcattt cggatgacat gtgtgatttg
     1201 gaatttcagg gtaccattat gaatgttatt tcatcattaa aacagcttaa cagacagcga
     1261 aatattaaaa caaaaaagat aaagacgaag gccagactgg catttctaaa aatgcgatac
     1321 agaaggaaaa catcattaca gccggatagt tataaaacag tgtgcatttt catgcacatg
     1381 caagccattg gtgacggcat cgttacctca gggtttatta aacaacttca aaaatccggt
     1441 atggtggtat atgtgatagc accttcaaga gtatcattcc tctttactga tattgttggc
     1501 gtggatgcct ttatatctta cgagaaaaat aaatttaacg aactaaaagc aaagataaaa
     1561 aaactgaatg tagatcttgt tgttgacttc tcaaattttg ataacacagc gataaccaga
     1621 ttgcaaacac tacatttact tagacctaaa cactctatct gttttaatca tcctgccgta
     1681 actatttttg atacaaatat aatcgacaat cgttccattc acacatctga aagaatgaaa
     1741 aaagttttat ctttactaaa gataaaaaat aataattatg ctgcagcgct taatttt
//
LOCUS       DAZFFI010000311         1761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFFI010000311 DAZFFI010000000
VERSION     DAZFFI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.313"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHJ12_005234"
     CDS             <1..315
                     /locus_tag="ACHJ12_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO4992853.1"
                     /translation="RTQSRRLKLSTRRARAAQFRDGWVQGIRQVVMPFSVTEEESRLM
                     RLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNASLHHGVGGTARQMVPGLMIRE
                     AS"
     gene            312..926
                     /locus_tag="ACHJ12_005235"
     CDS             312..926
                     /locus_tag="ACHJ12_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO4992854.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKGGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKVDED"
     gene            930..1298
                     /locus_tag="ACHJ12_005236"
     CDS             930..1298
                     /locus_tag="ACHJ12_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992855.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VASCIIEDGCMCVDGVGEYVSHSLPGGTYQLYTASPVPVVPEGLVKAVRFYEQVKREN
                     PPVETGAWKDAVDWVLKEAC"
     gene            1331..>1761
                     /locus_tag="ACHJ12_005237"
     CDS             1331..>1761
                     /locus_tag="ACHJ12_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFO4992856.1"
                     /translation="MQISPVTLRVAKAFISRHHRHNKPPVGHKFSIGLRNDAGELTGV
                     ATAGRPVARHFDDGLTLEVNRTCTTGERNANSALYGAVWRAAKDMGYRRCITYTRADE
                     SGASLRAAGFVRVKELPSGKSWAESSVALKSTRDPVGNGGVP"
BASE COUNT          403 a          390 c          579 g          389 t
ORIGIN      
        1 cgtacacaga gccggagact gaaactgagc acccgtcgtg cccgggcggc tcagtttcgt
       61 gatggctggg ttcaggggat tcgtcaggtg gtgatgccat tcagtgtgac agaagaggaa
      121 agccggctga tgcgtctctg gatggagcag caggatatgg tgacggtgga ttcccgggag
      181 atgaaagcct gtcgcggcgg tgacctggcc cgctggcagg ggtatgaggc cgggaaaaat
      241 gcctcactgc atcatggtgt ggggggaact gcccgccaga tggtgccggg tctgatgata
      301 cgggaggcgt catgaataac agcttgtctc aggcaaccaa cggtctgctg atgcaactgg
      361 tgatggacct caagagcggc tacctgcgtc gctgcgaatc tctggggctg gcccgggagg
      421 aaatgcagat gctgcaggga ctgaccattg aggaaattca ttacctgtca aactctgaag
      481 tgtcggttat ccgtctggac attaatcatg aaaacctgct gcgtatgctg cagcaggccc
      541 ggacggagca gaaacgtctg cagcgtattg accgggcact ggcgctggga ggctccattg
      601 agctgatggc gttttacttt ggactttcca gtgtggatgt ggcggcgcgc cggcgcattg
      661 ccggtattga tgttcgcccg ggacgtggca tcacactgag tgatgaggaa agtgcagagc
      721 tgtggcgttt atggcagaag ggcggcatca cagatgtgga aagtgcggac ggactggatg
      781 tcatgatgct ggcagctgag caaatgaata tcccgctgac ggcggtatgg catgcagtga
      841 ggggatggca tcagggaact gcggggttta atgatgtatc ggccagtcgc cggaaactga
      901 accgggaaaa agtggatgag gactaaccta tgaccactat taccaaagag cgactgcaat
      961 ggctggctaa catttctggc cgcgatgaca ttgacgatat agacggcggt gagattcgtg
     1021 agctggcgct tatcgcgctg gcatcgctgg aagcagagcc tgtggcgtca tgcattattg
     1081 aagatgggtg catgtgtgtt gacggggtcg gtgagtatgt aagtcactcg ctgcctggtg
     1141 gaacgtacca gctatacacc gcttcacctg tgccggtagt gcctgaagga ctggttaaag
     1201 cggtgcgctt ttatgaacag gtaaagcgtg agaatccgcc agttgaaact ggggcatgga
     1261 aagatgcggt tgactgggtg ctcaaagagg cttgctaggc tgtaaatatt cgcatcaaag
     1321 gagagtgagc gtgcagattt caccagtcac tcttcgtgtt gcgaaggcgt ttatatccag
     1381 acatcaccga cacaataaac ctccggtggg gcataaattc agtattggtc tgagaaatga
     1441 tgcgggtgaa ttgacaggtg tggcgacagc cggcagacct gttgcacgcc actttgacga
     1501 tgggctgaca ctggaggtca atcgtacctg tactactggt gaacgtaacg ctaacagcgc
     1561 actttatggc gctgtctgga gggcggcaaa agatatgggc tatcgccgtt gcattacata
     1621 caccagggct gatgaatccg gagcctctct tcgggcagct ggttttgttc gtgtgaaaga
     1681 acttccttca ggaaaaagct gggcggaatc aagtgtcgcc ctgaagagta cacgcgatcc
     1741 ggtcggaaac ggtggtgttc c
//
LOCUS       DAZFFI010000312         1736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFFI010000312 DAZFFI010000000
VERSION     DAZFFI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.314"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1736
                     /locus_tag="ACHJ12_005238"
     CDS             <1..>1736
                     /locus_tag="ACHJ12_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4992857.1"
                     /translation="MGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLS
                     QQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYS
                     FSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHR
                     AGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRT
                     LTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLS
                     LPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGATFEPLSGSLNSVIPPAV
                     QHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTSAITADTEHRF
                     RGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAAPDSIELTPGYYQITAVPVLAVY
                     DPTVQYEFWFSEKRITDTAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVG
                     KSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITET
                     RNEITQTVSKTM"
BASE COUNT          384 a          477 c          566 g          309 t
ORIGIN      
        1 atgggaaaac gcctgggggc cgcggacgtg gacaagtggg cactgtatgc catcgggcag
       61 tactgcgacc agacggtccc ggatggtttc gggggcacag agccgcggat gaccttcaat
      121 gcgtacctgt cacagcagcg taaggtgtgg gatgtcctgg gggatttctg ctcggcgatg
      181 cgctgtatgc cggtatggaa cggccagacg ctgacgttcg ttcaggaccg tccgtcggat
      241 gtggtatggc cctacaccaa cagcgatgtg gtggtggatg ataacggcgt ggggttccgc
      301 tacagcttca gtgccctgaa ggaccgacac acggcggtgg aggtgaatta caccgacccg
      361 cagaacggct ggcagacttc cacggaactg gtggaagacc cggacgccat cctgcgctac
      421 gggcgcaatc tgctgaagat ggatgcgttt ggctgtacca gccgcggtca ggcccaccgt
      481 gccggactgt gggtgataaa gacagaactg ctggaaacgc agacggtgga tttcacgctc
      541 gggtcacagg ggctgcggca cacgcccggt gacatcattg aaatctgtga taacgactat
      601 gccgggaccc tgaccggcgg acgcatcctg tccattgatg ccgccagccg taccctgacg
      661 ctggaccgtg aggtgacact gccggaagca gggacatcga cggtgaacct gattaacggc
      721 agcggtaagc cggtgcgcgt ggacatcact gcacaccccg cccctgaccg gatacaggtc
      781 agcgccctgc cggatggcgt ggagacatac ggtgtgtggg gactctccct gccgtcactg
      841 cgtcgtcgcc tgttccgctg tgtttccatc cgggaaaaca cggacggcac ctttgccatc
      901 acggcagtgc agcacgtacc ggaaaaagaa gccatcgtgg ataacggggc cacctttgag
      961 ccgctgtccg gttcactgaa cagcgtcatc ccgccggcag tgcagcacct cacggtggag
     1021 gtgagtgcct cagacggcca gtatctggcg ctggcgaaat gggacacgcc gcgggtggtg
     1081 aagggcgtgc gcttcagtct gcgcctgacc agcggaaacg gtgaaaacag ccgcctggtg
     1141 accagcgcca tcactgccga cacggagcac cgtttcagag gcctgccgcc cggggaatac
     1201 accctgacgg tcagggcgat aaacagctac ggccagcagg gcgaacctgc caccaccaca
     1261 ttccggatta atgcgcctgc agcaccggac agcattgagc tgacgccggg gtattatcag
     1321 ataacagcag taccggtgct ggcggtgtat gacccgacgg tacagtatga attctggttc
     1381 tcagaaaaac gcatcacgga cacggcacag gtggaaacct ctgcccgtta tctggggaca
     1441 ggcagtcagt ggagtgtctc cggcccgcac attaagccgg ggaaggattt ctggttttat
     1501 gtgcgcagcg tcaacctggt ggggaaatct gcgtttgtgg aagccagtgg ccgggcgagc
     1561 aatgatgcgg aagggtatct ggactttttc agaggagaaa tcgggaagac acatctggca
     1621 caggggatgt gggagctgat tgataacagc cagcttgatg atgagatggc ggagatgaag
     1681 accaccatca cagaaacccg caatgaaatc acgcagacgg tcagtaaaac catgga
//
LOCUS       DAZFFI010000313         1686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFFI010000313 DAZFFI010000000
VERSION     DAZFFI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.316"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            819..1421
                     /gene="fimB"
                     /locus_tag="ACHJ12_005239"
     CDS             819..1421
                     /gene="fimB"
                     /locus_tag="ACHJ12_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4992858.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          515 a          292 c          344 g          535 t
ORIGIN      
        1 caagctaact cagtgagcgt ggtgaaaatc agtgtttacc cgccatcagg ctgagcataa
       61 ttctcatcat gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt ctgaagtcga
      121 tctggagagg cttgttgatg ttggtgtttt caggatgatg tttcacttag tttgtttgcc
      181 gtatcgcccg gcgaatggct gtgattgagg aaggtttaag tcgtagtgac caaagctata
      241 tttaccaacg aatgtagatg aaaaaatcat ctcctgcgtt ccccatatct ccaggataaa
      301 aaggaatgta acaatctcat ggcgtaagct gacgaatcag ccaggaataa tcgctaggga
      361 cctaagaatt agcatgataa tagccactaa gaaattactg cgctccatga aatagccatt
      421 ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg aacgaatatt
      481 aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca catgatatat
      541 agataagatt agttgcatta atgatgaggg ttattattag attcgtatcc gattgataaa
      601 tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaacgtt attataatta
      661 actgttgcta ctccatttaa gttcactcag aagaactggt ccacttacgt tagttattaa
      721 gcaaacgttc gcttttataa acataatcag gataaaaatg ttggattatt gctaacccag
      781 cacagctagt gcgcgtctgt aattataagg gaaaaacgat gaagaataag gctgataaca
      841 aaaaaaggaa cttcctgacc catagtgaaa tcgaatcact ccttaaagca gcaaataccg
      901 ggcctcatgc agcacgtaat tattgtctga ctttgctttg ttttattcat ggtttccggg
      961 cgagtgaaat ttgtcgattg aggatttcgg atattgatct taaggcaaag tgtatatata
     1021 tccatcgatt aaaaaaaggc ttttcaacaa cgcatccgct attgaacaaa gaagttcagg
     1081 ctttaaaaaa ctggttgagt atccgtactt cgtacccgca tgctgagagc gagtgggtat
     1141 ttttatctcg taagggaaat ccgctttctc ggcaacagtt ttaccatatt atctcgactt
     1201 ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc cattcgtgtg
     1261 gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat cttgggcatc
     1321 gcaatattcg tcatactgtc tggtataccg ccagcaatgc agggcgtttt tacggcatct
     1381 gggatagagc cagaggacga cagcgtcacg ctgttttata gagatagttg agataccagg
     1441 gatggtgtta aaaactacat tattcttacg atatatcaaa aatgattaac catttattaa
     1501 ttgatgttat tgtaagtgtg attattaatt ttcataataa acattaagtt aaccatatcc
     1561 atacaaaata caatggttta tgttcttcaa aataaataaa caaaatcatt cataaattta
     1621 cacatcactt aaattctcct gtttccgcac ttttttcttt attttttaag caactggaag
     1681 ttaatc
//
LOCUS       DAZFFI010000314         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFFI010000314 DAZFFI010000000
VERSION     DAZFFI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.317"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..509)
                     /gene="bamE"
                     /locus_tag="ACHJ12_005240"
     CDS             complement(<1..509)
                     /gene="bamE"
                     /locus_tag="ACHJ12_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4992859.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKF"
     gene            complement(534..>1676)
                     /gene="sab"
                     /locus_tag="ACHJ12_005241"
     CDS             complement(534..>1676)
                     /gene="sab"
                     /locus_tag="ACHJ12_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Sab"
                     /protein_id="HFO4992860.1"
                     /translation="SAISKDDKGNYSYEIDVTGIPVEYTDSQGNPLVNIGGRFYTQTE
                     DAATGKLTMTPAEPARVRISSEQPLVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGD
                     NVVWKDGKAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAA
                     SSGQAAKAEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGSVAVGSHARAGRANT
                     GTYALNGQAVAGRTGSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNR
                     QVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGV
                     GGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAGVGWQW"
BASE COUNT          370 a          534 c          354 g          418 t
ORIGIN      
        1 tgaaacttct ctgccacacg ggaaagtgca tcacgccccc tctctgtcag agtccatgaa
       61 tcaaaatcaa ataatatatc accggggact gactccctgt attctgctgt ctctgcctcc
      121 ggcagacagt tttccggctt ccagtagaaa gagcgggcat ccatgttccg gtcaaacagt
      181 atcttgtact ggcaggtata atccccctcc ggtccggtaa agttgaacag gtaatcccat
      241 tcccttacac cagacatccc ttcagcaaag tgtggtctcc ccgccagctg ataaatctgg
      301 tccttattca tacccgcctt tatctgacgt aacttttctg gcagtggcca cgaacctttc
      361 tgtgttccgt catgattaaa catcaccttc tccggagaag gccataccac ttcttccgtc
      421 gttccttcat ccgtgacatg actcagattt ccacatcctg caagcatcag ggacagcaca
      481 cccgtcatca tcattcgact aaataacatt acatttctcc ttgcttttcc ctgttaccac
      541 tgccagccca caccggcacc gacggagaaa ttaccccggg tgttctgacc tgcattcagt
      601 ttcattatca tcttaccgtt atcggagatt cgggacacac ctattgcaat ggcgctttca
      661 ccaccatatc caccgacacc cagccccatc agacttttac ccggaagata ggcctgaggt
      721 atcccgctca tggccgcagc cgatgcgcca acaccacgga tatcccggtt aagctcttcc
      781 acccggtagc tcagctgact gaatttattc ccgagagtgt ttatcgcctg tgtatttccg
      841 gcaacctgac ggttagttgc atgcaactga ctgccattga cggcatccgt tgagttcgca
      901 gacaacacac ccggtgccac tgactgcagc tgacgctcct gtcccggctg accaaaagaa
      961 accaccgcag tatcacttcc ggtacgtccc gcaacagcct gaccgttcag ggcataagtc
     1021 ccggtattcg cgcggccagc acgggcatgg ctgccaactg caacagagcc ggcagcagcc
     1081 tgacgcgtga cttcagcacc attacccatg gcaatgctgt tttgtccccg ggaaacggcc
     1141 ccctgtccga cagcaatact gtcacgccct tccgcttttg ctgcctgacc ggaagaggca
     1201 gctttaaaat acttcgtgcc ttcacggttc agcatattca ctgcatcagc cacattatcc
     1261 ggtgttttat gtacaccacc attcaccgtt gtcttaccgc cacctgcagt tgtcagctca
     1321 ctgaaggtgt caggagccag cgctgctttt ccgtctttcc agacaacatt atcaccgcca
     1381 gcaattctgg ctgcagatgc cagctggctc ccgttaaccg catccgtgga tactggtgac
     1441 acatttccgg ccgccacatt tgtcagaaca agaggctgct cactgcttat tctgacacgg
     1501 gccggctcag ccggcgtcat tgtcagtttc ccggtcgcag catcttcggt ctgtgtgtaa
     1561 aaacgaccac caatattgac aagcgggttc ccctggctgt cggtgtactc caccggaatc
     1621 cccgtcacat caatctcata gctgtaattt cccttgtcat ccttactgat ggctga
//
LOCUS       DAZFFI010000315         1670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFFI010000315 DAZFFI010000000
VERSION     DAZFFI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.318"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1670
                     /locus_tag="ACHJ12_005242"
     CDS             <1..>1670
                     /locus_tag="ACHJ12_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4992861.1"
                     /translation="DQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVW
                     NGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGW
                     QTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGS
                     QGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVILPEAGTSMVNLING
                     SGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDT
                     PRVVKGVRFSLRLTSGSGENSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVE
                     TSARYLGTGRQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFF
                     RGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTL"
BASE COUNT          360 a          457 c          544 g          309 t
ORIGIN      
        1 gaccagacgg tcccggatgg tttcggggga acagagccgc ggatgacttt caatgcgtac
       61 ctgtcacagc agcgtaaggt ctgggatgtc ctgggggatt tctgctcggc gatgcgctgt
      121 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgtccgtc ggatgtggtg
      181 tggccgtaca ccaacagcga tgtggtggtg gatgataacg gcgtggggtt ccgctacagc
      241 ttcagtgccc tgaaggaccg gcacacggcg gtggaggtga attacactga cccgcagaac
      301 ggctggcaga cttccacgga actggtggaa gacccggacg ccatcctgcg ctacgggcgc
      361 aatctgctga agatggatgc gtttggctgt accagccgcg gtcaggccca ccgtgccgga
      421 ctgtgggtga taaagacaga actgctggaa acgcagacgg tggatttcac gctcgggtca
      481 caggggctgc ggcacacacc cggtgacatc attgaaatct gtgataacga ctacgccggg
      541 accctgacag gcggacgcat cctgtccatc gatgccgcca gccgtaccct gacgctggac
      601 cgtgaggtga tactgccgga agcagggaca tcgatggtga acctgattaa cggcagcggt
      661 aagccggtgc gcgtggacat cactgcacac cccgcccctg accggataca ggtcagcgcc
      721 ctgccggatg gcgtggagac atacggtgtg tggggactct ccctgccgtc actgcgtcgt
      781 cgcctgttcc gctgtgtttc catccgggaa aacacggacg gtacctttgc catcacggcg
      841 gtgcagcatg tgccggaaaa agaagccatt gtggataacg gggcccgctt tgagccgatg
      901 tccggctcac tgaacagcgt catcccgccg gcggtgcagc accttacggt ggaggtgagt
      961 gcctcagacg gccagtatct ggcactggcg aaatgggaca cgccgcgggt ggtgaagggc
     1021 gtgcgcttca gtctgcgcct gaccagtggc agtggtgaaa acagccgcct ggtgaccagc
     1081 gccatcacgg cggacacgga gcaccgtttc agtggcctgc cgcccgggga atacaccctg
     1141 acggtcaggg cgataaacag ctacggccag cagggcgaac ctgccaccac cacgttccgg
     1201 attaacgcac cggcagcacc ggccagcatt gaactgacgc cggggtatta tcagataacg
     1261 gcggtcccgc atcttacggt gtatgacccg acggtacaat ttgagttctg gttttcggag
     1321 gcaaaaatcg cagacacatc tcaggtggaa acctctgccc gttatctggg gacaggccgc
     1381 cagtggagtg tctccggccc gcacattaag ccggggaagg atttctggtt ttatgtgcgc
     1441 agcgtcaacc tggtggggaa atctgcgttt gtggaagcca gtggccgggc cagcaatgat
     1501 gcggaagggt atctggactt tttcagagga gaaatcggga agacacatct ggcacagggg
     1561 atgtgggagc tgattgataa cagccagctt gatgatgaga tggcggagat gaagaccacc
     1621 atcaccgaaa cccgcaatga aatcacgcag acggtcagta aaactctgga
//
LOCUS       DAZFFI010000316         1652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFFI010000316 DAZFFI010000000
VERSION     DAZFFI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.319"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(137..727)
                     /locus_tag="ACHJ12_005243"
     CDS             complement(137..727)
                     /locus_tag="ACHJ12_005243"
                     /inference="COORDINATES: protein motif:HMM:NF025659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO4992862.1"
                     /translation="MPEIVTEIRHARYDESGNIHAEMKLPSGECWMDIVVMPHDPDVI
                     WRELFAAIAGGQYGAVTPWRESQDMLEKEKEKACDRINIWRNEEENKEIVFEWQGRRW
                     DAGKRSLSRLEPVLAVTEHLPERFFWTDADNHDVAVDADELKALGVAMKQAMVAHGFR
                     IHERQRQMKQEIAGMTRYRDVLCYQPGWGSEDPVFT"
     gene            complement(1223..1652)
                     /locus_tag="ACHJ12_005244"
                     /pseudo
     CDS             complement(1223..1652)
                     /locus_tag="ACHJ12_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
BASE COUNT          379 a          513 c          341 g          419 t
ORIGIN      
        1 cacttgctta ggatgaatgg tcaggcctcg ggttaaacat gaatgtttgt ccggggcctt
       61 ttcacatccg gccttcaggt gttccctccg gccatcagcc aaaggcaccc gtgcataatg
      121 tacggttttt attctgttac gtaaataccg gatcctcact cccccagccg ggctgatagc
      181 acagtacatc acggtatcgc gtcatccccg caatctcctg tttcatctgt cgctgtcttt
      241 cgtggatcct gaaaccgtgc gccaccatcg cctgtttcat ggcaacaccc agcgccttca
      301 gttcatcagc gtctaccgcc acatcatgat tatccgcatc tgtccagaaa aaacgttccg
      361 gtaaatgttc tgtcactgca agcacgggct ccagacggct cagtgagcgc ttacctgcat
      421 cccagcgtcg cccctgccat tcaaacacaa tttctttatt ttcttcctca tttctccaga
      481 tgttaatcct gtcgcatgcc ttctcttttt ccttttccag catatcctga ctttcgcgcc
      541 acggtgtcac cgctccatac tgaccaccgg caatggcggc aaacagttca cgccatatca
      601 catcgggatc atgcggcatc acaacgatat ccatccagca ctcccccgac ggtaacttca
      661 tttcagcatg aatgtttccg ctttcgtcat acctcgcatg acggatttct gtaacgatct
      721 ccggcacaat ccccccttaa agcacatgaa ccttctcact tcctgcccgg aataacgtgc
      781 caccgttaca cagaacgctg atcgtcacgc gacagttata attcagggtg ttatgaaagg
      841 cgcgcacgta acgataccgg acataaatcc ggttgcggtt tcccctcccc tttgccggaa
      901 gcgtgaatct gtaaatccag ccataatttt cccacacctt atgcttcaaa ttcccgggcc
      961 cccattctcc tgacgaccag acagtggcaa ttccaccatc tgcactatca gtgccgacac
     1021 tgacttccca ccaggcagaa acaaaaccag gattgccctc agctctgaag gctatcttca
     1081 tgttaacatc aaaaaaacga tcgaagtttt ccccgtcaac attcagaaaa gggatccatt
     1141 cgttatctct ctcctttaca ccatcccgtg agctgacacc actgaacaca actttattga
     1201 tttcaaacag ggagcggtaa taaacatcgc caagaatatt accggcatcc actgttcctt
     1261 taacgacgca gttttcttca atggtcacgt tattcagggt gcccgagttc gcactgatat
     1321 gtccgctgat gtcagcattg cgggccgtca gcctgccctc cggcgtcagg gagaacgtcg
     1381 ggggattgcc ggacgaggtg atgctcaccg caaacagtcg cttcaggaac acatcgttca
     1441 tgaacagctg attcccctgc gccacaaaca gcggcgtggt gttgccgttc tccgggttaa
     1501 tcatcgcgat acggtccgcc tgcagcagaa tattgctcag gggctggcca tcagcatcct
     1561 caatccccgc accaataccg gcaacatacg gaatgccgtt tttcgttttc tgcaccttca
     1621 gcatgtacag cgcagccagg tcgtgatttg tg
//
LOCUS       DAZFFI010000317         1647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFFI010000317 DAZFFI010000000
VERSION     DAZFFI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.320"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..551)
                     /locus_tag="ACHJ12_005245"
     CDS             complement(<1..551)
                     /locus_tag="ACHJ12_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4992863.1"
                     /translation="MLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGG"
     gene            complement(715..855)
                     /locus_tag="ACHJ12_005246"
     CDS             complement(715..855)
                     /locus_tag="ACHJ12_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992864.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(1054..>1647)
                     /locus_tag="ACHJ12_005247"
     CDS             complement(1054..>1647)
                     /locus_tag="ACHJ12_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992865.1"
                     /translation="LFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNV
                     VIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIIIP
                     PIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGHGHIT
                     LSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          314 a          528 c          382 g          423 t
ORIGIN      
        1 cctcccggct cgcctttttc gcccttctct ccccgttcac ctttcacacc ctgaagcccc
       61 tgtggtcctg tctcaccacg ctcacccttt ggcccacgct cgccggtatc gccttttggc
      121 ccgggaatac cctgtggccc ggtgtctccc ttatccccct tcggtccccg cgcattctct
      181 gcccgtttct tcgcttcctc tgcactggcc gctgacgctt ctgcacgttt cagtatttcc
      241 gctgccaccg cttccagctc tgcaagggct ttcgggtaat actgtgcgtc ctccaggtcc
      301 atcagaaatt tattcagcgt tcccggtgca gaatccgcct tcaccagaat gtcacccaca
      361 tatgacggcg cgtacccttc cgtgttcagc gtcacccggt ataaccccgg ctcaacatcc
      421 atactgtaac tgccggtttc ccccggctga ccatacgcca ccgtggtgac aatcaccgtc
      481 tccgttgtgc ggcgcgcttt cagctctatc gtgcatcccg gtaccggctt accggccccg
      541 tctttcagca cacccgatat tctgactgtc attgatttcc cccataaaaa aaccgcagta
      601 ccggtttccc ggcctgcggt aaaatttgtg gtttgttggt gttaaaacgg tgccatccgg
      661 ctgaccaccc tcagcaaccg gtcggcgggg gatattctcc cccgccacgg tttcttactg
      721 cttacactgt aagaacgccg caatctccgc gcccgctatc cggaaccgga actcgcacag
      781 tgaagtgtgg gtgatccaga taatgagcac taccgtgata caaatcacgg tggtttttaa
      841 cggtttttgc gacataaacg cttgctcctt ttacggagag gcgctaacct tcaatttgct
      901 gaagatcgaa agtcaggcct cgggttaaac atgaatgttt gtccggggcc ttcgtctttc
      961 cggccttcag gtgttccctc cggccatcag ccaaaggcac ccgcgcatac tgtacggttt
     1021 ttgtctcctt ccggcaatcc cggggcgcga tgttcagcgg atgctgatcc ccgcgctgtt
     1081 tttcttcacc actatcgcct gaaggttact gatacgtgaa ctgccaaaac tcccgttctg
     1141 acgtcgtgaa cttacggtaa aactcagggt gatatgacca tgaccggctg gcatatcgat
     1201 gatcccgctg aaaattcccg gctctgtcac tgacctgccg gaataaatcc ggcgtccgtt
     1261 ctgatcaaca tgcaggaagc actctgtcca gatgtcatta ctggtacggg attcctgttt
     1321 tgacccgaca tagattatcg gcgggattat aatctgccgg tcaaagctgt gatcgtcata
     1381 caccgtcagc gttcgtgtac cgttcgcaag gtaactacca tccaccggaa aagccacccc
     1441 tgcacatttc acaagatcac caataatgtt ctccgctttc agcgtgccat ttatcgtaca
     1501 gttctccgct atcacgacat tattgagcgt gcccgagttc gcactgatat gtccgctgat
     1561 gtccgcattg cgggccgtca gcctgccctc cggcgtcagg gagaacgtcg ggggattgcc
     1621 ggacgaggtg atgctcactg caaacaa
//
LOCUS       DAZFFI010000318         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFFI010000318 DAZFFI010000000
VERSION     DAZFFI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.321"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..597)
                     /locus_tag="ACHJ12_005248"
     CDS             complement(<1..597)
                     /locus_tag="ACHJ12_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992866.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRDYRD"
     gene            720..1643
                     /locus_tag="ACHJ12_005249"
                     /pseudo
     CDS             720..1643
                     /locus_tag="ACHJ12_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          528 a          324 c          283 g          508 t
ORIGIN      
        1 atctctgtag tctctatatt tctcttcata cattccaagt tttggaggtt tgataggcgt
       61 tttaatttca ctgcgcaacg catctaagtt tgtaagatca ctgtctataa ttttaagata
      121 gtttaaatct aacgaaccat cagttttatc aagcttagtt ttcagccttt taacagcctc
      181 caaggaagca tcaagaactg gtgtctgatt cttcaaagct tctgaaaaac ttttttgtgt
      241 tgattttcga gcttgttgtt gagcagattt tcttaactct ttttcacgct taacttgttc
      301 taaaagctct ttacggtcat cagaacgtga accaaaaaat ctatcagcaa gttcagttaa
      361 caaattagat ataatagttt tcagttctct tgcagcctgg tttcttatga atccctctct
      421 ccccgactta tctttcagct ctttattact ggattgagta attccaatat aaccaaaaat
      481 ccttctatta gaccaataat atcgccctgc attccatgaa cgtctttctt ctatctggaa
      541 gaaatcatta tctactcgac cataaggtaa tactctcaag ctatccctaa aaatcattag
      601 tcctgcatac tttttggcct taagatcaaa gtgggaatgt tcacgttcag tatgtgatgt
      661 attttgtgaa aggaattcaa acgttcctat ctgaagctca aatgggccga cccctgcgta
      721 acatctctaa ggatgaatgg gaaaacctcc atgcattaaa agaaaagcgt ttaaatggaa
      781 tgcctaaaac aacacccaaa agtgaccagg ttattatgtt gcaaaatcaa tacgttcgtg
      841 aaatgcgaaa atatggcgta cgaggtttac gttatgatgc ggcaaaacac tcaaaacatg
      901 aacaaataga aagatcaata accccaccgc ttaaaaatta taatgagcgg ttacacaata
      961 ctaacttatt taacccaaaa tatcataaaa aagccgttat gaattacatg gaatatctgg
     1021 taacttgtca gttggatgaa caacaaatgt catcactgct ttatgaaaga gatgatttaa
     1081 gcgccattga tttttcattg ctcatgaaaa cgataaaagc cttttcattt ggtggagatc
     1141 tccaaaccct tgcatcaaaa ccgggttcca caatctcaag tatcccatca gaaagacgga
     1201 tattgattaa cattaaccac gattttccta acaatggtaa tcttttcaat gactttctat
     1261 ttaaccatca acaagatgaa caattagcaa tggcatatat ggccgctctg ccgttcagca
     1321 ggcctttagt ttactgggat ggccaagtat taaaatcaac gactgaaatt aaaaattatg
     1381 atgggtccac gcgtgttggc ggtgaggcgt ggcttaataa aggttgctct acctatcagc
     1441 agctctacaa tgaattccac gcattatata tagataaagc aggaatatgg agcgcatttg
     1501 agggtatatt tgcaactaaa aacgttctgg cctttagtcg tggggattct gtgaacatta
     1561 atcactctcc tcatgatgga ctagttataa taaataaagg aaacgaagaa gttgaaggta
     1621 cctggcctaa caaattgcaa cct
//
LOCUS       DAZFFI010000319         1631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFFI010000319 DAZFFI010000000
VERSION     DAZFFI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.322"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..572
                     /locus_tag="ACHJ12_005250"
     CDS             <1..572
                     /locus_tag="ACHJ12_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4992867.1"
                     /translation="ILDVCDRDSINTVISKIYSDEGRIDVLVNNAGVTRDNLINNMTE
                     QDWDIVIDINLKGVFNITQAVAPIMMEQNHGAIITMSSVVGTDGNIGQSNYAATKGGV
                     IAMSKGWAKEFSRKGAKVRVNCVAPGFVITPMTENLPEKVINHMIAKTPLGRMATPED
                     ISNGVLFLASEKSSFITGQTLKIDGGLVI"
     gene            582..>1631
                     /locus_tag="ACHJ12_005251"
     CDS             582..>1631
                     /locus_tag="ACHJ12_005251"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFO4992868.1"
                     /translation="MEKVYITGAKRTPIGCFGGSLSTVSASDLGAIAISAAIKQSRIN
                     VECIDEVIVGNVLSAGQGMGPGRQAAVKAGIPWKTPALTLNMICGSGMKTIIDAAMKI
                     RSGDMDVVVTAGIENMSQAPYLIDHHARSGVRMGNQVMSDSLITDGLTDAFNHYHMGI
                     TAENIARQFNITRYEQDTFALESQKRALKAIDNGEFEDEITPVQVHTRKGNIEVTTDE
                     YPRRDSSLEKLSVLRPAFEKNGTVTAGNSSGINDGAAALILASEKAIQTLELQPLAEI
                     RSFGQGGVDASVMGLGPVPAIDNALKKAKLDIKDIDLFEINEAFAAQAIGVLKSISEN
                     HSVSIDWLNQRTNVNG"
BASE COUNT          537 a          238 c          382 g          474 t
ORIGIN      
        1 taattcttga tgtttgcgat cgggattcga ttaatacagt aattagtaag atatattctg
       61 atgaaggtag aatagatgtt ttagttaata acgcaggagt tacaagagat aatctgatca
      121 ataatatgac agagcaagac tgggatattg taattgatat aaaccttaaa ggtgttttta
      181 atataactca ggcagttgca cctattatga tggaacaaaa tcatggggca atcataacaa
      241 tgtcttctgt tgtaggaact gatggtaata taggtcagag taattatgcc gcaactaaag
      301 gtggtgttat tgcaatgtct aaaggttggg ctaaagaatt ttcccgaaaa ggtgctaaag
      361 tacgcgtgaa ttgtgtagct ccaggattcg ttattacccc gatgacagaa aatctgccag
      421 agaaagtcat taaccatatg atagctaaaa cccctttagg gcgaatggca acacctgagg
      481 atatttcgaa tggtgttctt tttttagcta gtgagaaatc atcttttatt actggtcaaa
      541 cattaaaaat tgatggtgga ttagttattt aaggaggagg aatggaaaaa gtatatatta
      601 caggggcaaa aagaacgcct ataggatgtt ttggcggttc gttatcaaca gtgtcagctt
      661 cagatcttgg agcaattgcg atatctgcag caattaaaca gagcagaata aacgttgaat
      721 gtattgatga agttattgtc gggaatgttt tgtctgctgg acaaggaatg gggccaggta
      781 gacaagctgc tgttaaagcc ggtataccat ggaaaactcc ggctctgact ttgaatatga
      841 tttgtggtag tggtatgaaa actattatcg atgctgcaat gaaaattcgt agtggagata
      901 tggatgttgt tgttacggca ggcatagaga atatgagtca ggcaccttac ttgattgatc
      961 atcatgcaag aagtggtgta cgaatgggta atcaggtaat gagtgactct cttattacag
     1021 atggtcttac tgatgctttt aatcactatc atatggggat tacagcagaa aatatagccc
     1081 gacagttcaa tattacaaga tatgaacagg atacatttgc gcttgaaagt cagaagagag
     1141 ccttaaaggc cattgacaat ggagaatttg aggatgaaat tactccggta caagtacata
     1201 cccgaaaagg aaatattgaa gttactacag atgagtatcc tcgtagagac tcctcattag
     1261 agaaactttc tgtgttacgt ccagcatttg aaaaaaatgg taccgtgact gcaggaaatt
     1321 catctggaat aaacgatggt gcagcagctc ttattcttgc ttcagagaaa gcaattcaga
     1381 cattggaatt acaacctttg gctgaaattc gctcatttgg acaaggaggc gttgatgcat
     1441 cagtgatggg gctggggcct gttccagcaa tagataatgc attaaaaaaa gcaaaactgg
     1501 atattaaaga tattgatctt tttgaaatta acgaggcgtt tgcggctcag gcaatagggg
     1561 tcttaaaaag tatttctgaa aatcactcag ttagtattga ttggctaaat caaagaacaa
     1621 atgttaatgg a
//
LOCUS       DAZFFI010000320         1625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFFI010000320 DAZFFI010000000
VERSION     DAZFFI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.323"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..770)
                     /locus_tag="ACHJ12_005252"
     CDS             complement(<1..770)
                     /locus_tag="ACHJ12_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4992869.1"
                     /translation="MQHLPAPIHHARDVAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLK"
     gene            complement(772..1185)
                     /locus_tag="ACHJ12_005253"
     CDS             complement(772..1185)
                     /locus_tag="ACHJ12_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992870.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
BASE COUNT          365 a          469 c          437 g          354 t
ORIGIN      
        1 tttttcagca ttgtgacagc atcggactca ggcatcaaaa actgcacccg gtgccgggca
       61 gccacatcca gtgcaaacac cttcgtgtag acctccgttg agctgatgga cttatgcccc
      121 atcaggctct gcaggacctt cagcggtata ccggcataca gcatgtgcat cgcataggaa
      181 tggcggaacg tatgtggcgt gaccgggaca gagaacgtca caccatcagc ggcagcggcg
      241 gcaaccgctt ccccaatcca ggtcctgacc gttctgtccg tcacttccca gatccgcgct
      301 ttctctgtcc tgcctgttcg tttattacgc cgttccatgg ggattttcag tgtggccacc
      361 atcgtctgca gctggctgac gtaccaggag tcggagagcg gaaccagccg gtgagtctgc
      421 tgaccggctg gcattcttcc tgccgtcctg gcggcttttt ctgtccgctg cttcagagtg
      481 gccagctgca caaacggata cgggggcgtg agcgaaaaat ccccccgcgt cagcgccagt
      541 gcttcattaa tgcgcgcacc ggtgttccac agtgtggcca gcagcatctt gcggcgcaga
      601 tccgggacgt aatggagcag ggcgctcact tcaggggcca gcaggtattt tggcagttca
      661 tcatgaacca tcgacatctg gcggagtgcc agcgctgccg gataatcgat ggcaacagga
      721 agctgagcga catcccgggc atggtggatc ggtgccggca ggtgctgcat atcagtgttc
      781 actccacggg gaaacgtcgg gcaggttttc cacaacacgg tactttcccg gataacggag
      841 ctggttaccg ccacgcgtcc agacggtgac aacaaaaccc cctccctggt tgttttcggg
      901 gagcaggcga tcgcactgct cactcagcat gtggatcaca aacggcgcat cgctgccgtc
      961 ttcaaatacc agttccagcg catcgcggcc atgcagcgtt cccttactga tgatgacgta
     1021 ctcagccccc cgcatctccc tgagtaaatg ttttgcagca tccgggacaa gcagcctggc
     1081 tgcgccggca ttccagctga ggtagacata tccggcctgc gcctgtacag actgccagta
     1141 atccgttccg gcgacagcct gaccttcatt ctcaataaaa aacatgggtg actccttttt
     1201 cttttccggg cagggacggt catgcgataa cgtcatccat catcactgcc cttaccgtca
     1261 cgccgtctga taacatcatg ccgttatcat catcaccatg ataacaagca tccatataac
     1321 cacgccgtta aattaccgga gagccgtttc cgtcagatcc gactttattt gaataaacga
     1381 atttccggaa gtgtaccacg ctgaggaata gatcatattg ctgttttcag cccctacgcg
     1441 gcacatcaca ggacagggag atgacacaac atatagtgtc atgatcagct gaattcagca
     1501 cgacatgttg tgcttacagg gtgtggcgct gtgcggaggg ggaggaagat ccggaacagc
     1561 cggaaaccgg atctgcgcgg cgggaaacgg gcaggtgata tgataattaa gcaacacaca
     1621 taaat
//
LOCUS       DAZFFI010000321         1620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFFI010000321 DAZFFI010000000
VERSION     DAZFFI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.324"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..738
                     /gene="nanR"
                     /locus_tag="ACHJ12_005254"
     CDS             4..738
                     /gene="nanR"
                     /locus_tag="ACHJ12_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4992871.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(774..>1620)
                     /locus_tag="ACHJ12_005255"
     CDS             complement(774..>1620)
                     /locus_tag="ACHJ12_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4992872.1"
                     /translation="TISESKSLSGRIYQDPEHNGEFELDRVLTDIYAPQLIIFLPDKP
                     NGTGILIIPGGGYQAVMLDKEGTALAPEFLSQGYTLFVLSYRLPSPAEEENINKTFID
                     AISAMLYIRKNADKWHLQRHRIGVLGFSAGGHIASTFVNQICDFKRSSDSKEQEIFAI
                     PDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLHCC
                     DDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDWMY
                     SHGFK"
BASE COUNT          557 a          338 c          289 g          436 t
ORIGIN      
        1 tatatgtccg acaacaaaaa aaccgtgtta cccgggctaa caacgaccag aaagcccatt
       61 gttacaaaaa aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag
      121 gagataaggg aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt
      181 ggaagaccat ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat
      241 aacaagggag aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta
      301 tcaggcattg ttaaagattt tttatccaca gctgatggca tcaaggattt tgaaagacta
      361 cgcatctttt ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat
      421 attcacaggc ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg
      481 tatgctcaat atgatattga atttcatagt atacttgctg atatacctga caataaagta
      541 ttaaaaacaa tacactcagc attttctgaa tggctgataa tggccaaacc tctggaaaag
      601 aataaaatga catatgaaaa taacaaaaaa acccatcagc agcatattga aatacttacg
      661 gccataaaag agcatgattg tgataaagct gaacagctaa tgtataatca cctttataaa
      721 cgaattgaag catcatagtt actcgacgct tcaccaggta gtcaccataa atattattta
      781 aatccatgag aatacatcca gtccataaac agttttatcc atcctccaac aggaagatct
      841 gtggcccact gaacactaaa gccatgccct cctttctcat agagatgcat ttcagcagaa
      901 acgcctttct ctattaggcc acggtacatg agcagactgt tttcaattga cacaagatca
      961 tcatcacaac aatggagtaa aaacacagga ggaaaacatg atgtgacatt aagatccgca
     1021 ctatattgta ttatattttc tgaggtacta ttgtccccca gtaattgctt cctggagcct
     1081 ggatgtgtca catcatcttt cattgatatt acaggataaa ttaacccgac aaaatcggga
     1141 atagcgaaaa tctcttgttc tttactgtca gaagaacgtt tgaaatcaca aatctgattc
     1201 acaaacgttg aggcgatatg ccccccagca gaaaatccta atacaccaat acgatgacgc
     1261 tgtagatgcc atttatcagc attcttacga atataaagca ttgcagatat tgcatcaata
     1321 aaagttttgt ttatattttc ttcctcagct ggagatggta atctgtaact aagaacaaat
     1381 aatgtatagc cctgagataa aaactctgga gcaagagctg taccttcctt atctaacata
     1441 acagcctggt agcccccccc gggaataatc aatattcctg ttccatttgg tttatctggt
     1501 aaaaaaataa ttaactgagg agcataaata tctgtcaata cacgatcaag ttcaaattcc
     1561 ccgttatgtt caggatcctg ataaatacgg cctgaaagag atttactctc agatattgtc
//
LOCUS       DAZFFI010000322         1617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFFI010000322 DAZFFI010000000
VERSION     DAZFFI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.325"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1128
                     /gene="rhsB"
                     /locus_tag="ACHJ12_005256"
     CDS             <1..1128
                     /gene="rhsB"
                     /locus_tag="ACHJ12_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4992873.1"
                     /translation="IQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQ
                     SGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYT
                     PARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQY
                     DEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAK
                     SGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRY
                     FSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKD
                     NNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1100..1483
                     /gene="yhhH"
                     /locus_tag="ACHJ12_005257"
     CDS             1100..1483
                     /gene="yhhH"
                     /locus_tag="ACHJ12_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO4992874.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          514 a          316 c          416 g          371 t
ORIGIN      
        1 atacagaacg acagaacccg catccagacg atttatcagc cggggagctt cacgccactc
       61 atcagagttg aaaccgccac cggtgagctg gcgaaaacgc agcgccgcag cctggcggat
      121 gcgcttcagc agtccggcgg cgaagacggt ggcagtgtgg tgttcccgcc ggtgctggtg
      181 cagatgctcg accggctgga aagtgaaatc ctggctgacc gggtgagtga ggaaagccgc
      241 cgctggctgg catcgtgcgg cctgaccgtg gagcagatgc aaaaccagat ggacccggtg
      301 tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct gccgctggcg
      361 ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat atgatgaatg gggcaacctg
      421 ctgaatgaag agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat
      481 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      541 tatatcactc aggatccgat tggactgaag gggggatgga acctgtatgg atatcaattg
      601 aatccgatat cagacatcga ccccctgggt ttatctatgt gggaggatgc aaaatcgggg
      661 gcatgtacta atggtctttg cggcacacta tccgctatga taggtccaga taaatttgat
      721 tctatagata gcaccgcata tgacgcctta aataaaataa atagccaatc tatttgcgaa
      781 gataaagagt tcgctggttt aatatgtaag gataatagtg gcagatattt ctcaacagca
      841 cctaaccgag gagaaagaaa aggatcatat ccattcaata gcccttgccc taatggtact
      901 gagaaagtat cagcttatca tactcatggt gcagatagtc atggagaata ttgggacgaa
      961 atattttcag gtaaagatga gaaaatagtt aaaagtaaag ataacaatat caagtcattt
     1021 tatttaggta cgcccagtgg taattttaaa gcaatagata accacgggaa ggaaataaca
     1081 aacagaaaag gattacctaa tgtctgcaga gttcatggta atatgtaaaa aaatattgtt
     1141 taggaactgt gtcattgtat ctttgtttgt ttttacatac aacacatggg cgcagtgtaa
     1201 taataatatt aaaataatgc gcaagtatga aagtgaaggt aaatataccg ttagaaattt
     1261 ggttaaaaat aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg
     1321 acaggatgcc gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt
     1381 tgttgaaggt accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg
     1441 caaaaatgat gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca
     1501 aacatcgtgt gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa
     1561 aagatagcct ttatggaggt tcctgcaatg tcaaatacat accagaaaag aaaggca
//
LOCUS       DAZFFI010000323         1601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFFI010000323 DAZFFI010000000
VERSION     DAZFFI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.326"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(241..759)
                     /locus_tag="ACHJ12_005258"
     CDS             complement(241..759)
                     /locus_tag="ACHJ12_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992875.1"
                     /translation="MAGQQIFKRSVSCISLLSAVASGFYLLWSEWSAVKCQFDGMIFL
                     SFRNAGLFLLFFIILLIVVLFSVSMGVSGRYAVNPQIVTPGIIISLLLQCSAIMMVQA
                     WRWSPYALAGTFLLVQFWSGYLTGWVQRDLHEGRVKTGFRNTVSEFWLLIPLVLSGWG
                     ISWGYYTTLFTS"
     gene            complement(775..1317)
                     /gene="trbB"
                     /locus_tag="ACHJ12_005259"
     CDS             complement(775..1317)
                     /gene="trbB"
                     /locus_tag="ACHJ12_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO4992876.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQVRQEKAATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQLALQYGFSV
                     FPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGASDA
                     AGFMARMDTVLQMYGNKVSQ"
     gene            complement(1304..1591)
                     /gene="traQ"
                     /locus_tag="ACHJ12_005260"
     CDS             complement(1304..1591)
                     /gene="traQ"
                     /locus_tag="ACHJ12_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO4992877.1"
                     /translation="MCKYKFSLPRLDITGMWVFSLGVWFHIVARLVNSKPWMAYILAQ
                     VISAVLVLWGAYRVLDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
BASE COUNT          470 a          421 c          336 g          374 t
ORIGIN      
        1 cactgatagc tctcattaca gcatccgcaa tatggacaat taaggaacac ggcattaccg
       61 gttgttttct cattatccct catgttcttt tcggtcctgt tgtttttata tggtgctgtt
      121 tctgtattca gtcaacatca gaaaagttat tttcgttaaa gaagaaaaga aaccgacttt
      181 gtgttgcact ctgcattctg ttttttcagc tgaccacaat ctacagcata ttaaatataa
      241 tcatgaagta aataacgtag tgtaatatcc ccatgagata ccccacccac ttaataccag
      301 aggaataagt aaccagaact ctgacacagt attccggaag cccgttttca cccgcccctc
      361 atgtaaatcc ctctgaaccc atccggtcag atagccactc cagaactgaa caagaaggaa
      421 cgtgcctgcc agtgcataag gagaccagcg ccatgcctgc accatcataa ttgctgagca
      481 ctgaagaagg agagaaatta taatccccgg agtaacaatc tgagggttta ctgcataacg
      541 ccccgacaca cccattgaga cagaaaaaag aacaacgatt aacagtataa taaaaaacaa
      601 caaaaacaat ccggcattcc tgaatgaaag aaaaatcata ccgtcgaact gacatttcac
      661 cgcagaccac tcgctccaga gaagataaaa tccagaagca acagcactca gtaaagaaat
      721 acaacttaca cttcgtttaa atatctgctg accagccata aattctccct gatgttattg
      781 cgaaacctta tttccgtaca tctgcagaac ggtatccatc cgcgccataa aaccggcagc
      841 atccgatgcg ccctgtaaaa gcggcagcgc ttcaagcgta ttgacgttaa ccaggaaggt
      901 ggtcggtgtg gccaccggga tattcgggaa gaatgttttc agtacatccg gtggtgcagg
      961 taacgcctgc gggaaggccg tatcaccctg accatccagg gtatagggaa atacggaaaa
     1021 tccgtactgc agcgccagtt gtttcagtac cgggtcaaac tggtgacagt acggacatcg
     1081 cccctgcatg aacagcacca ctttccagtc agcaagattg acctgagttc cgttgctgag
     1141 gcggaaccag cgggttgctg ctttctcctg tcgcacctgt gcgtctgctg ccggctgagt
     1201 tgtccgtgct gccattccct ccggatgcca gagtcgctcg atttccgcac gtgtggatgc
     1261 ctgtgtacag ctggccagaa aaagcaggac aacggacagc gatttaacgg gaaacatgtt
     1321 catcatcatt atccttccgt tctctcagta acaactgctg tctgacttcc agtgcctcac
     1381 gctcttcctg actgacacgg gctatccacg tatcaagcac acgatatgct ccccacagga
     1441 ccagaacagc cgaaataacc tgagccagga tatatgccat ccagggtttg ctgttaacca
     1501 ggcgggcaac aatatggaac cagacaccca gggaaaatac ccacatcccc gtaatatcca
     1561 gccggggtaa agagaattta tatttgcaca taaatatatc c
//
LOCUS       DAZFFI010000324         1588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFFI010000324 DAZFFI010000000
VERSION     DAZFFI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.327"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..1508
                     /locus_tag="ACHJ12_005261"
     CDS             150..1508
                     /locus_tag="ACHJ12_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4992878.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          453 a          256 c          353 g          526 t
ORIGIN      
        1 tgtataaagc acttaggcaa taataattac attcagcaac tatcatcatc ggtattgttt
       61 gtgggcggaa tttcatattg acaacagtac agttcttata tctattaata atagaaaggg
      121 atctacaacc tacagattgg tgtagcttta tggaaaaaga ctatttgaga attagtagta
      181 ctgtattagt gagcttattg tttgggcttg ctttggtttt ggtgaatagt tggtttaatc
      241 agccaggcgt tgaggaagtt gttccacgat caacctatct gatggtaatg attgctttat
      301 tttttatcga tactgttgca tttattttta tgcagttgta tttcatttat gaccgtaggc
      361 aattttcaaa ctgtatactt agtttggctt ttctgagttg tttgatttac tttgttaaaa
      421 ccgtcattat cattcagcaa attattgagg ggcgtttgac aagcagtgtt gtccaaaatg
      481 acattgcaat ctattatttg tttcgtcaga tgagtttgtg catattaata tttctggcat
      541 tggtgaataa agttagtgaa aacacaaaac agcgcaattt attttccaaa aaaatgactt
      601 tgtgcataag tttgtttttt gtttttgggg ggccgatagt tgctcacata ctttctagtc
      661 attatgagag ctacaattta cacattgccg agttaaccaa tgaaaatggt caggtggttt
      721 ggaaagcctc atatgttacc ataatgattt tcatgtggtt aaccttgctt tcggttaatc
      781 tatactttaa tggtttacgc tatgatatct ggaatggagt gacggtgatt gctttttgtg
      841 ccgtactgta caatatctct ctgttattta tgagtaggta tagcgtgtcc acttggtata
      901 ttagtcgcac tattgaagta gtcagtaagt taactgttat ggtaatattt atgtgccata
      961 tattcagtgc gctacgagta acaaagaaca ttgcacatcg cgatccctta accaatatat
     1021 ttaacagaaa ttattttttt aatgaactga cagttcaatc agcatcagcc aaaaaaacgc
     1081 cttattgcgt catgattatg gatatcgacc acttcaaaaa agtcaacgac acctgggggc
     1141 atccggttgg cgatcaggtg ataaaaacag tggtgaatat cattgggaaa agcatacgac
     1201 cagatgacct tttagcgcgc gtcggcggcg aagagtttgg cgtcttgctg acggacatcg
     1261 atactgaacg tgcgaaagct ttagcggaaa ggattcggga aaatgttgag cgtttaactg
     1321 gcgataatcc tgaatacgct ataccacaaa aagtgacgat tagtattggc gctgttgtga
     1381 ctcaggaaaa tgcgttaaac ccgaatgaga tctatcgact ggctgataac gcactttacg
     1441 aggcgaaaga gaccgggcgt aataaggtgg ttgtgaggga tgtggtgaat ttttgtgagt
     1501 caccataaag cggcattttg atcctaccca cgtaatatgg acacaggcct aagcgaggtt
     1561 cttgttttca aattgttccg gactgagg
//
LOCUS       DAZFFI010000325         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFFI010000325 DAZFFI010000000
VERSION     DAZFFI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.328"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(250..630)
                     /locus_tag="ACHJ12_005262"
     CDS             complement(250..630)
                     /locus_tag="ACHJ12_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992879.1"
                     /translation="MINNEWQYEFFRLRSAGCRDDARELLYSIPPSNEADCYFVGQHF
                     FEFEEYYPAIEMLTYCIDFGYKNNSTWYRSMAYLLRAYSFAKIGKYSEAEKDISYLDD
                     EIKVAWLPHPEKEISKAVITSMLR"
     gene            complement(647..>1573)
                     /locus_tag="ACHJ12_005263"
     CDS             complement(647..>1573)
                     /locus_tag="ACHJ12_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992880.1"
                     /translation="WYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKA
                     RHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQ
                     MEDAYIPERRLHLYHCDHRGLPLALISPEGETAWCGEYDEWGNQLNEENPHHLYQPYR
                     LPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYIYPLSPVMNIDPMGLS
                     VMAPALSMQNLSALLKSKMESTISSCNGESSGYLLTCQTAYDSDCGEIHERCMMLCKT
                     AQIDPDMSHIWGGSLFGCLNGCLPARCGGNAV"
BASE COUNT          393 a          435 c          321 g          424 t
ORIGIN      
        1 cgaattattt agtacaaaaa gtgtacttct gatgatcttt ccctgggtgg gcctgccacc
       61 atatcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat ctttgtgcct
      121 acggcacacg atgatctttc cctgtgtgac agagttgata gtacaaaacg gggagcagaa
      181 tgggtgtaaa catagttctc aggtaattcg gtacagtgtg atttgaattt tccaccccaa
      241 aaaacgtttt tatcttaaca tggaggttat taccgcctta ctgatttcct tttctggatg
      301 agggagccat gcaactttaa tttcatcatc taaataagaa atatcttttt cggcttcgct
      361 atatttaccg atttttgcaa acgaataagc gcgtaaaaga taagccatac tccgatacca
      421 agtggagtta ttcttataac caaaatcaat acaatacgtc aacatttcta ttgccggata
      481 gtattcttca aattcaaaaa aatgttgtcc aacaaagtaa caatcagctt cattcgatgg
      541 aggaatggag taaagaagtt cgcgggcatc atcccgacac ccggcagaac ggagacggaa
      601 aaactcatac tgccattcat tattaatcaa tatgatatcc tcataattat acggcatttc
      661 caccacatcg agcaggtaga caaccattta gacacccaaa aagagaaccg ccccaaatgt
      721 gtgacatatc tggatctatt tgtgcagttt tacataacat catacaccgc tcatgtattt
      781 ctccgcagtc actatcgtat gctgtttggc aggttaataa atatccagat gattccccgt
      841 tacaagacga aattgtcgat tccatcttac ttttaagtaa tgcggataag ttttgcattg
      901 acaatgctgg agccataaca gatagcccca tcggatcaat gttcataacc ggactcaacg
      961 gataaatgta taaattccat cccccctcca gcccaatggg atcctgggtg atatacctcc
     1021 cctgcaacgg atcatagtac cggttccggt tgtagtacag ccccgactcc tcatcatgct
     1081 gctgccccgg cagacggtat ggctgataca ggtgatgcgg gttctcctca ttaagctggt
     1141 tgccccactc atcatactct ccgcaccacg ccgtttcccc ttccgggctg atgagcgcca
     1201 gcggcagtcc ccggtggtcg cagtggtaaa gatgcagcct ccgctccggg atgtatgcat
     1261 cctccatctg cgcggccatc tgttccgccg tcagtccgca ctgcgcaagc catgcctcac
     1321 tctccgcact caccgctccc gcgcgcagtt cccgctccag ccttcccagc atcaccgcca
     1381 gctccgccgg tagcgtcaca cccgtgtcct cctgcaacac ctccgccagg ctacggtgcc
     1441 gcgccttcgc ctgttcgcca ttctctgttt cgattctcag gagcggcgtg aagcttcccg
     1501 gctggtatac cgtctggatt cttgtctgtt gggtctgtac agtggtcagc ctgtcaccgt
     1561 cccacccgta cca
//
LOCUS       DAZFFI010000326         1543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFFI010000326 DAZFFI010000000
VERSION     DAZFFI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.329"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1073)
                     /locus_tag="ACHJ12_005264"
     CDS             complement(<1..1073)
                     /locus_tag="ACHJ12_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4992881.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRA"
     gene            complement(1122..1460)
                     /locus_tag="ACHJ12_005265"
     CDS             complement(1122..1460)
                     /locus_tag="ACHJ12_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4992882.1"
                     /translation="MEFILLSVVLGIVPAIIAQTKGRSFFVWWIYGTLLFIVALVHSI
                     VITKDEKTHEQEMMGNGMKKCHFCAELIKEEAIKCKHCGSDLTNSNDTVCQKTYEEYL
                     QEARRKAGLE"
BASE COUNT          373 a          473 c          321 g          376 t
ORIGIN      
        1 tgagcccggt acacggctgc ccttgcccgc gccgtggcaa tctgcgttcc ccttaactgg
       61 gcctcagcca gtgccacttc attacgtgca gcggtcacaa gtcctgccgt ggcagacatc
      121 gctccggaag ccagattacc aaagtaccgg gccaccccga cagcaaccag cacacccgca
      181 gctgttgcca cggtatcaat atttccggcc acaccgttca gcgcgccgga gagcgttttc
      241 gtcgctccgc tggcctcatt cgcgccgccc acccaggcca taaaggcgtt ttccaccttc
      301 gtgatcccgt cagacaccgt ttccggcatg gctgcatatt catcacgcaa tatccccagc
      361 tggctgatta acgcgggaac gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc
      421 ttcagatcct tacgggccac acccatgcct gcagccagtg cgcgaatgat ccggtcccca
      481 ctttcattga ccgaattaaa ttcctcgccg cgcaacaccc cctgtgccag cgcctggctg
      541 aactgggtga tcaccgaccc cgcctctgcc gcactggcac cggaaatttt cagccctgtc
      601 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcattgag
      661 gcggcagagc gggcaaacag ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg
      721 atatccatca gcactttctg tgatgacgaa aattcatccg atgactgcga cgcctgtttc
      781 agacgggcat tcacggagct ccattcatcc gccagcgaaa tcaggtgtcc ggtggcaaag
      841 gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
      901 ttcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg acgtccgcca
      961 ttctgcaggg tccggtaata ttcactgccc atacgggatg cccgctggat ctccgactgg
     1021 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
     1081 tctccggacg aaaaaaaacc gccgcagcgg ttttcaactt ttcattcaag accggcttta
     1141 cgtctggcct cttgaagata ttcctcataa gttttttgac aaacagtatc attactatta
     1201 gttaaatcac taccacaatg cttacattta attgcctcct cttttattaa ttccgcacag
     1261 aaatggcatt ttttcattcc attacccatc atttcttgct catgtgtttt ctcgtcttta
     1321 gttatcacta ttgaatgaac caaagcaact atgaaaagta gagttccata tatccaccat
     1381 acaaaaaatg atctaccttt agtttgtgca ataatagcag gaacaattcc taacacgact
     1441 gaaagaagaa taaactccat caacacctcc tgatttaata aaattatatt aaaatattaa
     1501 tatcaggagt tcattgagca actgcacata atgcatcttc cag
//
LOCUS       DAZFFI010000327         1541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFFI010000327 DAZFFI010000000
VERSION     DAZFFI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.330"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(101..1507)
                     /gene="gndA"
                     /locus_tag="ACHJ12_005266"
     CDS             complement(101..1507)
                     /gene="gndA"
                     /locus_tag="ACHJ12_005266"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4992883.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQLAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          386 a          384 c          370 g          401 t
ORIGIN      
        1 gttaatatta agcagtgaaa tttagtctat aagataaatg gcaaaaaaaa gcccggtgca
       61 atatacgccg ggcctcaatt ttattgttgg ttaaatcaga ttaatccagc cattcagtat
      121 ggaacacacc ttctttatca atgcgcttat aagtatgtgc accgaaatag tcacgctgtg
      181 cctggatcag gttcgcaggc agaacagcgg cacggtagct atcgtaatag gcaaccgcag
      241 cggcgaaggt cggaaccggg ataccgtttt gtactgcata agcaacgaca tcacgcagcg
      301 cctgctggta gtcatcggca atttgcttga agtacggagc cagcagcagg ttagcgatct
      361 gcggattttc ggcatatgca tcggtgattt tctgcaggaa ctgcgcacgg atgatgcagc
      421 cagcacggaa aatcttcgcg atttcgccgt agttcagatc ccagttgtac tcttcagacg
      481 cggcgcgcag ttgagagaac ccctgagcgt aagaaacgat tttgcccaga tacagtgcac
      541 ggcgaacttt ttcgatgaac tcagccttgt cgccagctag ctgcgcttgt ggcccagaga
      601 gaactttaga cgcagcaacg cgctgatctt tcagagaaga gatataacgt gcaaacacag
      661 actcggtaat cagcgacagt ggttcaccga gatccagcgc gctctggctg gtccatttgc
      721 ccgtaccttt gtttgctgct tcatccagaa tcacatcaac caggtagtta ccgtcttcat
      781 cttttttggt gaagatatct ttggtgatgt cgatcaggta gctgctcagt tcaccgttat
      841 tccattcggt aaaggtctgc gccagttctt cgttggtaag gttcaggcca ccttttagca
      901 gagaataggc ttcggcaatc agctgcatat caccgtattc aataccgttg tgaaccatct
      961 tcacataatg acctgcacca tcggcaccaa tataggtaac gcatggctca ccgtcttcag
     1021 ccactgcggc gattttggtc aggatcggtg caaccagttc ataggcttct ttctgcccac
     1081 caggcataat ggaaggacct ttcagcgcac cttcttcacc gccggaaaca ccggtaccaa
     1141 tgaagttaaa gccttctgca gaaagctcac gattacgacg aatggtgtcc tggaagaagg
     1201 tgttaccacc atcaatgatg atgtcgcctt tatcgagata tggtttgagg gaatcaatag
     1261 cagcatccgt gcctgcacct gctttcacca ttaacaggat gcgacgaggc gtttccagag
     1321 attcaacaaa ctcttttacc gtatagtaag gaaccagttt cttgcctgga ttttcggcaa
     1381 tcacttcttc cgtcttttca cgggaacggt tgaaaataga gacggtataa ccacggcttt
     1441 cgatgttgag cgcaaggttg cgccccatca ctgccatacc gactacgcca atctgttgct
     1501 ttgacattgt ttactcctgt cagggtttta ccgcgtgtgc g
//
LOCUS       DAZFFI010000328         1538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFFI010000328 DAZFFI010000000
VERSION     DAZFFI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.331"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..636
                     /locus_tag="ACHJ12_005267"
     CDS             <1..636
                     /locus_tag="ACHJ12_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4992884.1"
                     /translation="EDAVNELWKLKRRGEADNKGSMWWLIQAGESEPVSPVPKVTAQM
                     LIKAIEQHGPQTADELALMFGITSRRANSSLAMAISKGRLIRVNQGGKFRYCIPVADL
                     PAEPEAASVAETDGKAFPQPAGVALPVREAETQEDIKTETVADIVQSLPSFTETRADD
                     LVLPSLHVANRELRRAKGQVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            669..950
                     /locus_tag="ACHJ12_005268"
     CDS             669..950
                     /locus_tag="ACHJ12_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4992885.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            947..1252
                     /locus_tag="ACHJ12_005269"
     CDS             947..1252
                     /locus_tag="ACHJ12_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4992886.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            1239..>1538
                     /locus_tag="ACHJ12_005270"
     CDS             1239..>1538
                     /locus_tag="ACHJ12_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4992887.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAEN"
BASE COUNT          405 a          331 c          482 g          320 t
ORIGIN      
        1 gaagatgcgg tcaacgaact gtggaaactg aagcgccgcg gtgaagcgga taacaagggt
       61 tcgatgtggt ggctgattca ggctggtgaa agtgaaccag tgtcaccggt accgaaagtg
      121 acagcgcaaa tgctgattaa ggcgattgaa caacatggcc cacaaacggc ggatgagctg
      181 gcactgatgt tcgggattac ctcccgccgg gcgaattcat cgctggccat ggcaatcagc
      241 aaagggcgtc tgattcgcgt gaatcagggc ggtaaatttc gttactgcat accggtcgct
      301 gatttaccgg cagagccgga agctgcatcc gtagcggaaa cggatggtaa agcctttcct
      361 cagccagcag gtgttgcgtt accagtccgg gaagcggaaa cacaggaaga tattaaaaca
      421 gaaactgtgg ctgatattgt gcaatcgttg ccatcgttta ctgaaacgcg agcggatgac
      481 ctggttttac catcgctgca tgtggctaac cgcgagctgc gccgggcaaa aggtcaggtt
      541 cagaagtggg agcgagtctg tgccgcgctg cgggaactga acaaacacag ggatattctc
      601 cgggatatta ccgccaccag agagctgctg cggtgagtgg ctggaagaag tggcgctggg
      661 cggaaattat gatactccgg cagtgtgcgg gaacgatgac agtcgaaagc atcggcagtc
      721 tgattggccg tagtggggct gccgtcagga cgaaagcgcg ggaactgggt atctgcatga
      781 tgttacgggg tgattatcac cagtcagcca aatgttcaca gcgtgatatt gagctggcgc
      841 ggcaactgca tcagcgtggc gtaccccgac gggaaattgc cgaaaagttt gggatgaagt
      901 tgggcgcagt gaataactac gtttatttcg acaggagggt tcaggcgtga gggtgagaat
      961 ttatatcgcc ggtccgatga cgggatatga aaatttcaac cgtgaggcgt ttcacaaggc
     1021 ggaagaagca ctgaaacagg aagggcatac cgtattaaat cccgcagtac ttccggacgg
     1081 gctgactcag ccacattaca tggatatttg tatggcaatg attcgttgtg tggatgcggt
     1141 ttatatgctg aaaggctggc agcggtcggc aggcgctaag gcagaactgg cactggcgga
     1201 gaaactgggg catgcagtta ttttccagga ggcaaccagt gagaaaaatt aattatcaga
     1261 cgctacgtga gatagcaaaa ctggcaaccc agggcgaatg ggtcgcattc atttcgccgg
     1321 gcactgatac atatgcggtg catacgccgg gggatgaacg ttgcggagac attatcaaat
     1381 ggcctggctt tgatgaccag aaaaatgcag agaacaacgc agaatttatt gcagctttca
     1441 accctgaagt agtacaggca ctgctggatg aacgggaagc ccaaagcaaa cgtattgccg
     1501 agctggaagc taatcttgtg gcgctggctg cggagaat
//
LOCUS       DAZFFI010000329         1513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFFI010000329 DAZFFI010000000
VERSION     DAZFFI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.332"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(284..433)
                     /locus_tag="ACHJ12_005271"
     CDS             complement(284..433)
                     /locus_tag="ACHJ12_005271"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4992888.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQLGASVARIAQEHDIND
                     NLLFK"
     gene            497..775
                     /locus_tag="ACHJ12_005272"
                     /pseudo
     CDS             497..775
                     /locus_tag="ACHJ12_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1151..1513
                     /locus_tag="ACHJ12_005273"
                     /pseudo
     CDS             1151..1513
                     /locus_tag="ACHJ12_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          412 a          325 c          371 g          405 t
ORIGIN      
        1 tccatttttc tttcttccag tcaggactgc atctgattaa tcgtaagcgc tccgggaagt
       61 acgtcgcgta aggattattt tacagctgag aagtttcagt gcagcagttc atgcacacgg
      121 ttcgacggct agtcattgat cttcataatt acctcgcgta aaaaacgggg taaggccgct
      181 catccagcgt catctccaca ggtaataatt caactccagt gtcagaagag gttgttaccg
      241 aaagacgccg agatatacgc ccttcgtttt gccagagtct gagctatttg aacaggaggt
      301 tatcattgat atcgtgttcc tgggcgatac gggcaacaga ggctcctagt tgcaaagcca
      361 gtttaaccat ttgaagttta aaattatttg aaaatgatct gcggggttct gcggataatg
      421 ttttttgctc cataacaggc gtccactagt ttgaaaaggt gggcacctac gttaccaata
      481 ctggcttaat ggctacatac ggcggtcggt ttacgcttac gaaatacggg gattaccgga
      541 gtcagagcgt ctttccgtca ggcagcagcg aagcaaagtg ttactgacgt cgctgcatga
      601 atggatggtg gagaagaatg gtacgctgtc gaaaaaatcc agactgggcg aagcgttcag
      661 ctatgtactg aatcagtggg atgccctctg ttattacagt gatgacggtc tggcggaggc
      721 ggataataat gctgcggaaa gagcgcttcg tgcagtctgt ctcggaaaga aaaactctta
      781 tgacctttgt caaatattga gcccaaaaag accttacgca gctccgggag cttcgttata
      841 tcgcggatta aagaacctga aacagtcaga ctgtatcttg gattttacaa actcttattc
      901 ggggatttta gccccatgac gatttacgca gtgagctggc ctctttctat atgcaggtat
      961 tggcgcattc tgacctcctg aattctttta cgaggatcac tcattccgtg tctgactgct
     1021 tcagggttta attttgcttc tgctgactga atgatgattg ggcagcaagt gcaatatccc
     1081 acataatgag gtagcctgag tttaacggac actccttcct gaaatagaat ggcatcagaa
     1141 ggagctaata atgagcagaa aaacccaacg ttactctaaa gagttcaaag ccgaagctgt
     1201 cagaacggtt cttgaaaatc aactttcgat cagtgaaggc gcttcccgat tatctcttcc
     1261 tgaaggcact ttaggacaat gggttaccgc cgccagaaaa gggctcggaa ctcctggttc
     1321 ccgcacggtg gctgaactgg aatctgaaat tctgcaactg cgtaaggcgt taaatgaagc
     1381 tcgccttgag cgagatatat taaaaaaagc aacagcgtat tttgcacagg agtcgctgaa
     1441 aaatacgcgt taatcgaaca atggcgacaa caatttccca ttgaagcgat gtgtcaggta
     1501 tttggtgtat cca
//
LOCUS       DAZFFI010000330         1494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFFI010000330 DAZFFI010000000
VERSION     DAZFFI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.333"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..416)
                     /locus_tag="ACHJ12_005274"
                     /pseudo
     CDS             complement(61..416)
                     /locus_tag="ACHJ12_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            complement(420..782)
                     /locus_tag="ACHJ12_005275"
                     /pseudo
     CDS             complement(420..782)
                     /locus_tag="ACHJ12_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
BASE COUNT          324 a          362 c          403 g          405 t
ORIGIN      
        1 ttttcgatgt gatcgtcgta ggatattggt aagggagagc tgaaaaaact ggggaagtag
       61 ttatgctgcc atggcggata actttcctcc actctgagcg tctatcgtgc aacccgggta
      121 ccaactggcg aatttgtcct gcagttaatt attcaggaca gcggttctgg tctgtaacaa
      181 ataatgcgta ccttccttac cccactgcat ctgttgcttt tgggtcatcg tctggcgatc
      241 acttcgttga ttgtcgactc tacaaacgtt gttgaaaccg gttcaccata ccggcgcatt
      301 tttccgtagt ttgggagcat catctgatta ttacggatgc aggtatacat ttcatccagc
      361 tgtttttgca gggatttcag gtttgcatag ttgagttcag ggttatcgca gtacataagc
      421 tgcagcaatt cctgatgata tgcagtaata gcgtttacca cccctgacgt catccgtgtg
      481 tcggcgatca gctgagtgtg ccagcccttc ccatgtcagt ccggcctgct ggcagcacag
      541 gccagagaaa tcgtgccata gcggttggtg gccccataat atcctgctgc cagttccatc
      601 gcacaatata ctgtcagttc ttccagccag tcagccggat cgctatgtgc ggcagcagtc
      661 tgtttcagaa tgcgggtgtc gaaccgggca ttaaagataa ttactggtcg gtctccgatc
      721 gcatggcgca gttgcagagc cacatctgtc tatgatggcg cgccgtggag agccagctca
      781 tttcttgacg gtggggcgta attgctccat tttcgtacag gttctcttcc gcttcctcgt
      841 cgattcgctc actacgttcg gtcatcgatt tgcggcgagc ggggtctcct ctcatatatc
      901 gttgacgatt acgatttgat ttatttcgat cgagccgttg gaggctcgtt aaatgactta
      961 aattgttctg taacgcgctc aatagcgtca gggcatggga ggtgagaaaa tgcgttacgg
     1021 gactttcagg aggtcaggca cgccgatact gttgcggagc agaatctgtc cgtagccgtg
     1081 ccacggccag ctacagatca gacgcacgca gggtacgaag ccagcatctt cccggagacg
     1141 gaaggaggcg tgtaggtttg gccagtatac cggagcttcc ccacttcgct ccagcacccg
     1201 ttccatgtcc cgcgtcagca ctggcctcgc aggggggagt agaactactg acattcggac
     1261 gactgtaccg gcttacgccg ggattcggcg gttttactcc tctgccatat acagcatgga
     1321 gtgccgcctt ccatgttgct ggcgcggcat atcatggtga cgatatctga ataattatat
     1381 actgtgtata tacgtggtaa tgacgaggtg atgaatggca caggttaata tgagtttaag
     1441 aatcgacgct gaactgaagg atgcttttat ggggtcagcc tggaattcgg aaaa
//
LOCUS       DAZFFI010000331         1487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFFI010000331 DAZFFI010000000
VERSION     DAZFFI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.334"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..399
                     /locus_tag="ACHJ12_005276"
     CDS             <1..399
                     /locus_tag="ACHJ12_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4992889.1"
                     /translation="TVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQ
                     AIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVN
                     LKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            413..653
                     /locus_tag="ACHJ12_005277"
                     /pseudo
     CDS             413..653
                     /locus_tag="ACHJ12_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            673..1004
                     /gene="sctI"
                     /locus_tag="ACHJ12_005278"
                     /pseudo
     CDS             673..1004
                     /gene="sctI"
                     /locus_tag="ACHJ12_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1001..>1487
                     /gene="sctJ"
                     /locus_tag="ACHJ12_005279"
     CDS             1001..>1487
                     /gene="sctJ"
                     /locus_tag="ACHJ12_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO4992890.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLTVYEKDINPEI
                     KI"
BASE COUNT          515 a          245 c          298 g          429 t
ORIGIN      
        1 acagtgagta agcaacggaa cgctatcaca cagcaactaa tcgataatct tattaaagga
       61 ttactgcaaa ctatgccata tgccagcaat ataagtattg cggtattaga tgataatgta
      121 ttggaaagtc aggctattga aacgttgtca gcgataggtc tttcttatga aaaatataaa
      181 acagctaaca atgtgtattt caatatcatt ggtacgttaa gtgacagtga attaaataaa
      241 attaataact atgttgacga atattataaa caatggggta aacaatatgt aagatttaat
      301 gtgaatttga aaaatcagga cacaaataat agttcattta gctacggaga taaccgattc
      361 gagaagtctc aaggtagcaa ctggacattt caggaataat tgaggaggac ttatggcaga
      421 ttggaatggt tatattatgg atatcagcaa tcaatttgat cagggcgttg atgatctgaa
      481 ccagcaagtt gaaaaagcgt tggaggattt agccaaccaa tccctccgac acgaaatttc
      541 ttgccgaata tcaaagtgca ttagctgagt atacattata tcgaaatgcg caatctaacg
      601 ttgtaaaagc gtataaagat cttgattcag caatcataca aaacttccgc taaactggat
      661 aaggatttta ttatgtcagt aagcaatatg ccacctattg atagagctga gcaatcaact
      721 gctcatgaaa tacagcaagc gaaagtgatt gatttaaatg accgggttct caatcttgac
      781 aacccggatg ataaaatgat ctccgctttt gccaactatg ctgtacaaac tgaaaattgg
      841 cagcaaaatg cgttacaagc attaaggtct gataaagagg ggttaactcc tgaaaaatta
      901 ttagtcttgc aggatcatgt gcttaattat aatgtcgagg tttcattggt ggaacattag
      961 caagaaagat tgtcgctgca gttgagacgt tgacccgctc atgaaatata tatcattatt
     1021 attgtttatc ctgctgttgt gcggctgcaa gcagcaggaa ttattgaacc atcttgatca
     1081 acagcaagct aatgatgtcc ttgctgtttt acaaagacat aatattaatg ctgaaaagaa
     1141 ggatcaaggc aaaacaggat tttcaatatt tgtcgaacca acggattttg cttctgcggt
     1201 ggattggcta aaaatctaca atctaccggg taagcctgat attcaaatat cgcagatgtt
     1261 ccctgcagat gcactcgttt catctccacg agcggaaaaa gcaaggctat attctgctat
     1321 tgagcagcgg ttagagcaat ctctcaaaat tatggatggc attatttctt cgagagtaca
     1381 tgttagttac gatgttgata atggcgatag tggtaaaact gctttaccaa ttcatatttc
     1441 tgtacttaca gtatatgaaa aagatatcaa cccagaaata aaaataa
//
LOCUS       DAZFFI010000332         1486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFFI010000332 DAZFFI010000000
VERSION     DAZFFI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.335"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(245..1375)
                     /gene="intE"
                     /locus_tag="ACHJ12_005280"
     CDS             complement(245..1375)
                     /gene="intE"
                     /locus_tag="ACHJ12_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4992891.1"
                     /translation="MAARPRKNNVSVPNLYPLYSRKVNKVYWRYKHPVTGKFHSLGTN
                     EAEAIAIATEANSRLAEQRTRQILAISDRIATSKGKAITTSTWLDRYQAIQDDRLESG
                     DIKLNTYKQKAKPVSLLRERAGMKLISSVDVRDIAQLLDEYITAGQPRMAQVVRSVLI
                     DVFKEAQHYGEVPPGYNPAQATKQPRRKITRQRLSLEEWQKIFDIADASHRYMGNAML
                     LALVTGQRLGDISRMKFSDIWDDHLHVIQEKTGSKIAIPLSLRLNAINWSLRDVVARC
                     RDYAVSPYLVHFFRTTSQAERGAQVKSNTLTTNFSRARDLAEIDWGNGSPATFHEQRS
                     LSERLYKEQGVDTQKLLGHKTQQQTDRYHDDRGKDWMYIHQF"
     gene            complement(1356..>1486)
                     /locus_tag="ACHJ12_005281"
     CDS             complement(1356..>1486)
                     /locus_tag="ACHJ12_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003841900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4992892.1"
                     /translation="KQGRLWRVREDAELVGELVTPVIKKSDSIILQRILSNGSQTT"
BASE COUNT          366 a          323 c          307 g          490 t
ORIGIN      
        1 ctagaatagt gtctatgaaa taactctttg caaatggtgg ccatattcca tttccgcgct
       61 gataatcctt gttaattatt agttcttttc tctcaagcat tgaaattaga tcaattaatg
      121 tgatctgccc tacatttgta ttcatgacta ttctcctgac tatgaaaagg agggttattt
      181 tacatcaata ataaaattgt tcaatattca tagtttatag ggagtctttt gaagaggttc
      241 atagttaaaa ctggtggata tacatccaat cttttccccg atcatcatga taacgatcgg
      301 tttgttgttg cgttttatgg ccaagtagtt tttgtgtgtc taccccctgt tctttgtaca
      361 gacgttcaga taaagacctt tgttcatgga atgttgctgg tgagccatta ccccagtcaa
      421 tttctgccaa atctctcgct ctactaaaat tcgtcgtcaa tgtattggat ttaacctgcg
      481 cgccacgttc agcctgtgaa gtggtacgaa aaaaatgcac aaggtatggg ctgactgcat
      541 agtcacggca gcgggcgact acatcgcgta aactccagtt aatcgcgttg agacgaagag
      601 aaagcgggat ggcgattttg ctcccggttt tttcctggat gacatggaga tgatcatccc
      661 aaatatcgct aaatttcata cgcgagatat cacctaacct ctggccggta accagtgcta
      721 acagcatggc attccccata taacgatgac tggcatctgc gatatcaaag attttttgcc
      781 attcttcaag acttaaccgt tgtcgggtaa tttttcttct gggttgtttg gttgcctgtg
      841 ctggattata gcccggaggg acttccccat agtgttgtgc ctccttgaat acatcaatta
      901 gaacggagcg gactacttgc gccatcctcg gttgcccggc agtgatatac tcgtcaagca
      961 attgcgctat atcccttaca tcaactgatg aaattaattt cattcctgct cgttccctga
     1021 gcaaggatac tggtttggct ttctgtttat aggtgttgag ctttatatcg ccactttcca
     1081 gtctgtcatc ctggattgct tgatagcgat ctaaccaggt tgacgttgtg attgcttttc
     1141 ctttgctggt tgcgatcctg tcactgatag ccagaatctg ccgggttctt tgctcagcca
     1201 ggcgtgaatt tgcctcagtg gcaatagcaa tggcttcagc ttcgtttgtg cctaaagaat
     1261 gaaacttccc ggtcactgga tgtttatatc gccaatagac tttatttact tttctactat
     1321 aaagtggata caagttcggg actgaaacat tatttttacg tggtctggct gccattactt
     1381 aaaatccttt gtagaataat agaatcactt ttcttgatga caggagtaac taattcccca
     1441 actaactctg cgtcctcacg tactcgccat agtcgacctt gtttca
//
LOCUS       DAZFFI010000333         1474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFFI010000333 DAZFFI010000000
VERSION     DAZFFI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.336"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..>1474
                     /locus_tag="ACHJ12_005282"
     CDS             54..>1474
                     /locus_tag="ACHJ12_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO4992893.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMG"
BASE COUNT          459 a          211 c          395 g          409 t
ORIGIN      
        1 tatgtacagt gtgttgtttt taatttattg ttttaattat caaagaggaa tttatggcta
       61 aagaactaag tggatatggc ccaactgctg gtgagtctat ggggggaaca ggggcgaatc
      121 tgaatcagca aggtggaaat aataatagta atagtggggt tcactggggt ggtggttcag
      181 gctctggtaa tggaggtggt gaacatggaa gtcagacggg gtggggatgg agtaaaacca
      241 ataatccgga tgtgccgcct tatgttgatg ataatgggca ggtgcgcatt accattacaa
      301 atggtctggt aaaaacgcct gtatatggag ttcctgaggc tggcggaaat agtgatgttc
      361 aggggggcta tattcctgaa aatcctaatg atgaagttgc tcgtaagtgg gataaaaaca
      421 acttaccgcg agagattgat gtgagtatcg atggttttaa atatcgtgta acgcttaatg
      481 ataatggtag agctatcggt attcttcgaa caggtgtccg tccttacgta gggagtgaaa
      541 aagctaaggc gggtattatg gaaaaaatca atcataaaac acctgaggag atatatgagg
      601 cgttaggttt taataaagat gaatctcagc ggcaggaaaa agcaaaacaa caggctgaag
      661 atgcgtggga tcgtttgcct cctaatgtga gaaaatttga tgttgatgtt gaacagttcc
      721 actatttagt ggtgcttgat gattatggta atgttttatc tgtaactaga acagctgttc
      781 gtccttatgt agggagtgaa aaagctaagg ctggtattat ggataaagtc gaccataaga
      841 ccccggaaga gatatacgag gcattaggat ttaataatga agagcctcaa cgacagaatc
      901 aagctaaaaa agctgcttat gatgtatttt attctttcag tatgaaccgt gaccggattc
      961 agagtgatgt gctaaataaa gcagctgaac ttatatctga tatgggtaac aagatcggtg
     1021 attatcttgg tgaatcttat aagtcgttag caagagaaat tgctgatgat atcaaaaatt
     1081 tccaggggaa aaccatccgt agctacgatg atgcaatgga gtcattaaat aaaattttgt
     1141 ctaatcctag ttttaaagtt aacagagcag accgtgatgc actggtgaac gcctggcgaa
     1201 gtcgtgatgc tcaggatata gcaaataaac tggggaatat cagtaaggct ttcaaagctg
     1261 ccgatgtaat tatgaagatt gagaaggtca aggagaaaag catcgaggga tatgaaactg
     1321 gaaactgggg gccgctgatg ttggaggttg agtcttgggt tctcagtggt atggccgcaa
     1381 gcgttgccat gacattgctt agtgctataa ttggatcatt cacactaccg gcgttagcat
     1441 taactgcaat aaatattgca ggtattatgg gaat
//
LOCUS       DAZFFI010000334         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFFI010000334 DAZFFI010000000
VERSION     DAZFFI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.337"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..236)
                     /locus_tag="ACHJ12_005283"
     CDS             complement(<1..236)
                     /locus_tag="ACHJ12_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4992894.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTNFRHRTIIAFAVDG"
     gene            complement(242..499)
                     /locus_tag="ACHJ12_005284"
     CDS             complement(242..499)
                     /locus_tag="ACHJ12_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4992895.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            635..913
                     /locus_tag="ACHJ12_005285"
     CDS             635..913
                     /locus_tag="ACHJ12_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992896.1"
                     /translation="MAQDTKLHNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            915..>1464
                     /locus_tag="ACHJ12_005286"
     CDS             915..>1464
                     /locus_tag="ACHJ12_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992897.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAACNQVVDALPEAV"
BASE COUNT          350 a          385 c          387 g          342 t
ORIGIN      
        1 ccatcaacgg caaaagcaat aatcgttctg tggcggaagt tagttacccg cattccctgg
       61 cgaatatcat cgcgtttatt gccccgatgc gggaatgtgg aaaacccatc aagataatca
      121 agaagtgcat tggcataatt gtcagcaatg acgctccctg ctttctccgt tatatatctg
      181 tgcaggttga ttatctgctg ttcggcctca ggagtaatga tgacttcata tgtcatgcag
      241 attacttccc ggctcgaatc gcagcgcgaa cctgtgaaat ggagcgtccg ttgtttggat
      301 tttcgcggat agaatcgaga gatggggcgg ctgaatgcgt taaccacgct tcgattgctt
      361 tatcgcgctc attcagtgcg cgaagccctt cacgaatgac ctcgctttct gaagcatagg
      421 caccggaagc cacacgggcg cgcaccatgt cagccatctc gttagttaat gtaatgctga
      481 attgttgggt tgtacgcatg gtaaacctca cggagtagga tagaacacta ttcgatgata
      541 gcacgctgcc tgttgacgac aacagaaatc agagacaata ttgccgcacg ccagcctgaa
      601 caactggcac ctgctgcgcc agcagagaca accgatggcg caagatacca aattacacaa
      661 ttcggataac tccgccgccc ctgccagcag gcacgggcgg cgttctcatg cattcaaatc
      721 tgactggttc cagcatgacc catgcactga agaacaggcc gaatggctga tccagtgcta
      781 ccgcagacgt ggttacgagt ttcagaaaga tctcagcttc gatcgtcgtc actggataat
      841 ctccgtcagg ctcccttatt ccgaacgccc accgcgtccg tcccgcacat tccagcagcg
      901 tatctggagg taacgtgcgg gtattacttc gacctgttct ggtaccggaa ctcgggatgg
      961 tgctccttaa gccgggccgt gaatcaatgt cagcattcca taacggcaga atattggtgg
     1021 agccggaacc gaaaaacatg cgcggtctgc cgtccggagt cgttcctgcc gttcgccagc
     1081 cgctggcaga ggataaaaca ttactgccat ttttcagcga tgagcgggtt attcgtgcag
     1141 caggtggtgc aggtgcactg tctgactggt tattacgtca cgtgaaatcc tgccagtggc
     1201 cacacggcga ttatcatcac agcgaaaccg tcattcaccg ttacggtacc ggcgcgatgg
     1261 tgttgtgctg gcactgtgac aaccagctgc gcgaccagac atcagaatca ctcgatcaac
     1321 ttgctcagca gaatctggtt gcctggatga ttgacgttat ccgtcacgca ataagcagta
     1381 cgcaggagag ggagttatcg ctggccgaat tatcctggtg ggcggcctgc aatcaggtgg
     1441 tggatgcact acctgaggca gtag
//
LOCUS       DAZFFI010000335         1459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFFI010000335 DAZFFI010000000
VERSION     DAZFFI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.338"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..572)
                     /locus_tag="ACHJ12_005287"
     CDS             complement(105..572)
                     /locus_tag="ACHJ12_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992898.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRIYQARIKAS"
     gene            732..986
                     /locus_tag="ACHJ12_005288"
     CDS             732..986
                     /locus_tag="ACHJ12_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFO4992899.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            986..1402
                     /locus_tag="ACHJ12_005289"
     CDS             986..1402
                     /locus_tag="ACHJ12_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO4992900.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
BASE COUNT          391 a          339 c          323 g          406 t
ORIGIN      
        1 taatcaataa aaaccgtgcg aaaaacggga caggatgtgg ccattaatgc taatgtatgc
       61 atacattagc attaatggcc ctgacaaata ccaggcatcc ggtctcagga tgccttgatt
      121 cttgcctggt agatgcgctt tacccgttca atttctgcca gcgttgcgga ccagtgtagt
      181 tcggcggcat gacgatatct ggcagccatt ttttccacca tctccggatt gctgtccgcg
      241 ctgcggtgct gacgcccctt ctccccaaaa acttcaacaa ccagccactg gcttcctgtt
      301 ctgccgaaca atgccttgca caaacgtctc acagtcagcg gggttaaatc tggatcggga
      361 tcgtaggcag tggtagcaaa aataaagcgc agcatttctg atgtcttgta acgctttacg
      421 gcagctgcca gacgtgcggc gcgttggggt gcttcccagc ggtcgtcact gtaattagca
      481 acttcagcca gataatcctt ttcaaggttt tcagccgtgt aacacctgcc gcctaaaaaa
      541 aaccgcatgg cctccctggt tttgtctctc acatatcatc cctcattaat gctaatgtat
      601 gcatacatta gcattaatat ctgacagcaa caagcggcga agactacccc taatcaagta
      661 catttcttgc attgtactat ttcatgccct acaatgtagt aacgtaccta tatacatcaa
      721 aaggcaacag catgaagatt acaaccctat ccagtcggga acttaatcaa gatgtaactc
      781 gagccaaacg cgccacgaat tatggtccag tatttattac tgatcggggt aaaaccgcac
      841 atgtacttct tagtatcgaa gaataccgaa aattgacgca acaacgacga actctcattg
      901 acattctggc gatgcccgat gctgatgcag ttgaatttga accgcctcgt attacggtta
      961 gtttaaaacc tgcggagttt ttatagtgta tattcttgat actaatgtta tctctgagct
     1021 acggaaggct cggacaggac gtatagataa taatgttgca aaatgggcat tgcatacaga
     1081 tgcggcagat ttctttattt ctgttatttc cataatggaa cttgaacagg gaatattaca
     1141 agttgaacgt cgagatatgc gacaagggat tatgctacgt tcttggatgg atcagcatgt
     1201 gttaccagcg ttttctgata agatacttcc tgtagatact gcggttgcat tacgttgttc
     1261 tcgtttacat gttcccgaca ggcgcagtga acgagacgca ttgatcgcag caacggcgct
     1321 ggtacatggg atgacggttg taaccagaaa tgttggtgat tttgaattaa ccggagtatc
     1381 aattattaat ccctgggctt gatatcgctt cccttcttct aggggtccgc ttggaaaacg
     1441 gaaattttcc cacgctaag
//
LOCUS       DAZFFI010000336         1454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFFI010000336 DAZFFI010000000
VERSION     DAZFFI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.339"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..1044
                     /locus_tag="ACHJ12_005290"
     CDS             58..1044
                     /locus_tag="ACHJ12_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4992901.1"
                     /translation="MYRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITSGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1359..>1454)
                     /locus_tag="ACHJ12_005291"
     CDS             complement(1359..>1454)
                     /locus_tag="ACHJ12_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO4992902.1"
                     /translation="PFVKALTSNALVGLSTCATSTECFGPDRKKN"
BASE COUNT          425 a          271 c          356 g          402 t
ORIGIN      
        1 acactttctt ttaataatga aaatatttaa ttaaatctat tatgtaaagg gggagtgatg
       61 tatcgtcatg attttaatga aaaaatagat ttctggtatg tgatagctct cgcaggacag
      121 tctaatggta tggcctacgg tgaaggtatt cccctgccag atacactcga caaacccgag
      181 tcccgtgtaa aacaactggc tcgtcgaaaa acaatcacat caggaggaaa agagtgtaaa
      241 ttcaatgaga ttattccggc agatcactgc ctgcatgatg ttcaggatat gagtggatat
      301 caccatccgg ctgctgattt gcataaaggg gaatatggtt gtgtcggaca gggattgcat
      361 atagctaaaa agttactgcc atacatacct gagcaagcag ggatattact ggttccttgt
      421 tgcagaggag gtgccgcctt tactgttgga gctgaaggta tgtatgtacc tgatacaggg
      481 gcaacagctg atgcaatgcg gtggggcacc ggaacagcac tctatgagga cttagtagcc
      541 cgggttaagg tagctctgga atataacaga aagaataaac tgctatctgt ctgctggatg
      601 cagggggagt ttgatcttat gtcaccagat tatgaaaagc atcctgactt gttttatcag
      661 atggtaactt ctttcaggtc tgagttatct gaatactcat ctcagtgtgt tggtaattcg
      721 tcagaaagag ttccatggtt gtgtggtgat acaacctggt actggaagga atcttatcaa
      781 aaagaatatg attttatata tggacattac agacaacgga ctgatgatga aatacatttt
      841 ctcagctttc aggatagtaa ccgacatgaa ttaactaatg agccagaaga agatgccgat
      901 gatttatctg tcgggtatct ggggtcatca tggaggacag aattaagctg gacaacgtca
      961 cagagaagta cacactttaa ttcaatggcc agaaggggag ttattgctga atgttatgcg
     1021 caaaaaataa gaaattattt gtagacaagt tcaggatata tctacagctc tgacatgatt
     1081 ctctgagaga catgacggag tggtatggag ttgtcggtat caacaccagg atagctggtc
     1141 gttaccgttt gatatcactg gtgctgatac tatttggacg gtagtgtgtc acgaaggcac
     1201 gcaccaggga cgtgagtccc tgtaatatca tcgacagtcc cattatggca tgtctgcacc
     1261 tacaacgtca tttcgaggtc ggctcagtgt tcactcgagt cttatatcac tgacgccgac
     1321 actccatcca gagtatctct ggacggaaac atcacctatt agttcttttt tctgtcgggg
     1381 ccaaaacatt cagtcgatgt ggcgcaggtc gataatccaa caagggcatt ggacgttaat
     1441 gccttcacaa aggg
//
LOCUS       DAZFFI010000337         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFFI010000337 DAZFFI010000000
VERSION     DAZFFI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.340"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..340
                     /locus_tag="ACHJ12_005292"
     CDS             83..340
                     /locus_tag="ACHJ12_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992903.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPENDR"
     gene            340..930
                     /locus_tag="ACHJ12_005293"
     CDS             340..930
                     /locus_tag="ACHJ12_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992904.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(950..1297)
                     /locus_tag="ACHJ12_005294"
     CDS             complement(950..1297)
                     /locus_tag="ACHJ12_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO4992905.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          359 a          385 c          396 g          279 t
ORIGIN      
        1 atattatcag aaatcgataa cacaaaaatc atatagaatt tgatttttac ctgtcagaac
       61 attatcagat ggagcgcgca ggatggatga agaaagggtg ttttcactca gctatgaaca
      121 actgacccgg tttgcggaaa aacgcatccg ggaatgcaat ctggacagcc agggcgcgat
      181 ttatctctgc gagtcagcca aagccggagc cgtgctgatc ttctggcatg aactggccat
      241 aaatggctat gcctcaatga atgccataaa gagacaggag ctcattgacg ccgactttca
      301 gcgcctcaga aacctcatct ggccggagaa tgacaggtaa tgacccaaag ccgccgaccg
      361 tcgccactgc agcggagagt gctgattgtg ctggctgccc ttgatgaaaa acggccaggc
      421 cccgtgctaa cgcgggacat agaacgggtg ctggagcgaa gcggggaagc cccggtctac
      481 gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg ccggctggct gcgtaccctg
      541 cgcgctccga acctgcagct ggcagtggag ctgacggacg ccggccgggc cgtggcacag
      601 cctctgctgc tggcagaaca ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc
      661 gtactgccgc tgatcccggc agcaggtctg cccgcagacg gcacttctgc cactgatctg
      721 gcggtggagc tgaacggcat aacgtatcag gcctgccgtg gggattttgt ggtgcgtctc
      781 gacggcagca cctgtctgca gttatggaat aaagaaggca gggtggtacg ccgggagggc
      841 gatccgctgg aagtggcgca gtggctgcag gcctgtcatg acgcaggaat agaagttcgc
      901 gtacagataa atgaaagtgc cgctccgtaa gaacacgcct gtgcacggtt tacacatcat
      961 cccatggtgg cagccggtta actttcctgc gccaccagtg gaaagaggcc atgcacagtc
     1021 cgaacaaaaa accccccgtg atacttataa taatggcctc acctgccacc attcctgacg
     1081 gcccccagta gataaaccac attgcacatc cccaggaaat gccccataag ccccccatca
     1141 gcaccgtgac ctgccagaac ggcataaagg gtaatggcag aagccggata cccagccgcc
     1201 agagaatacg cagaagagga ggggcataat tgctccgcca catctttttg ctgtccatca
     1261 gggcaatggc ccgggctttt ttctgctcaa aggtcacagg agcacctccg ttccatgatg
     1321 taaacgtgac acaacttatt gttattgatt attattatca tgctgtggtt atgttgtcat
     1381 gcatcagtac attgacaaaa gtgcaaaaga aaggaaaac
//
LOCUS       DAZFFI010000338         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFFI010000338 DAZFFI010000000
VERSION     DAZFFI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.341"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..542
                     /locus_tag="ACHJ12_005295"
     CDS             63..542
                     /locus_tag="ACHJ12_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992906.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWITYSLYKARTGSTVGGGLQFMLLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(468..>1419)
                     /locus_tag="ACHJ12_005296"
     CDS             complement(468..>1419)
                     /locus_tag="ACHJ12_005296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992907.1"
                     /translation="TAHFLISVATGNIAHFLITVATSNTAHFLTSVATGNTAHFLISV
                     ATGNTAHFLISVATGNTAHFLTSVATSNTAHFLITVATGSTVHFLTSAATGSTVRFLT
                     SAATGSTAHFLISAATGSTVRFLISAATGSTVRFLTSAATGSTVHFLTSAATGSTVRF
                     LTSAATGSTAHFLISVATGNIAHFLITVATSNTAHFLTSVATGNTAHFLISVATGNTA
                     HFLISVATGNTAHFLTSVATGNTAHFLITVATGSTVHFLTSAATGSTVRFLTSVATGN
                     IAHFLISVATGNTAHFLITVATGNMVNDDYYLNVQRQKQH"
BASE COUNT          447 a          232 c          427 g          313 t
ORIGIN      
        1 gcgactggtt aaaaagaaaa cagaatcggg aactggtcag gtgaaacaag gtggtaaata
       61 gcatgcagaa atacaaacag gaaagtctga gtgcagccat gaaagaaagt ggcctgttga
      121 tcgctaaaat ttgtgcctgc gtgattgccg gagtggtggt tatctgggga ctggtgacag
      181 gcacagagac cctgctcact gcgctgaaaa caatgcacga acagtacgat aacagtatgt
      241 gttatatcgt atgtgccaca gggtggtttt tcctggtgtt aagtaactgg ctgtggatca
      301 catacagtct ttataaagcg cgtacaggca gtacagtcgg agggggcctg caatttatgc
      361 tcctcctgag tctgctggca gaatgctttt cattttttgt gattttctgg tcattgtctc
      421 atctggatat aaccgacatt cagttgctcg tgatggggat aacgacatta atgctgtttt
      481 tgtctctgaa cgttaaggta gtagtcatca ttaaccatgt tgccagtggc aacagtgatt
      541 aaaaaatgag cggtgttgcc agtggcaaca gagattaaaa agtgagcgat gttgccagtg
      601 gcaacagagg ttaaaaagcg aacagtgctg ccagtggcag cagaggttaa aaagtgaaca
      661 gtgctgccag tggcaacagt gattaaaaaa tgagcggtgt tgccagtggc aacagaggtt
      721 aaaaagtgag cggtgttgcc agtggcaaca gagattaaaa aatgagcggt gttgccagtg
      781 gcaacagaga ttaaaaagtg agcggtgttg ccagtggcaa cagaggttaa aaagtgagcg
      841 gtgttgctag tggcaacagt gattaaaaag tgagcgatgt tgccagtggc aacagagatt
      901 aaaaaatgag cagtgctgcc agtggcagca gaggttaaaa agcgaacagt gctgccagtg
      961 gcagcagagg ttaaaaagtg aacagtgctg ccagtggcag cagaggttaa aaagcgaaca
     1021 gtgctgccag tggcagcaga gattaaaaaa cgaacagtgc tgccagtggc agcagagatt
     1081 aaaaaatgag cagtgctgcc agtggcagca gaggttaaaa agcgaacagt gctgccagtg
     1141 gcagcagagg ttaaaaagtg aacagtgctg ccagtggcaa cagtgattaa aaaatgagcg
     1201 gtgttgctag tggcaacaga ggttaaaaag tgagcggtgt tgccagtggc aacagagatt
     1261 aaaaaatgag cggtgttgcc agtggcaaca gagattaaaa agtgagcggt gttgccagtg
     1321 gcaacagagg ttaaaaagtg agcggtgttg ctagtggcaa cagtgattaa aaagtgagcg
     1381 atgttgccag tggcaacaga gattaaaaaa tgagcagtg
//
LOCUS       DAZFFI010000339         1402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFFI010000339 DAZFFI010000000
VERSION     DAZFFI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.342"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..350)
                     /locus_tag="ACHJ12_005297"
     CDS             complement(45..350)
                     /locus_tag="ACHJ12_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4992908.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(347..742)
                     /locus_tag="ACHJ12_005298"
     CDS             complement(347..742)
                     /locus_tag="ACHJ12_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4992909.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGADWHSVR
                     RCLVDVLACGDLYMSGKYGVFTSEQVYRVWRKTPEKTTDQTLIRKLPDGEIRRYDRHQ
                     NIICCECRNSEVMQRVLAFCQGNFQEVME"
     gene            complement(758..>1402)
                     /locus_tag="ACHJ12_005299"
     CDS             complement(758..>1402)
                     /locus_tag="ACHJ12_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4992910.1"
                     /translation="EDAVNELWKLKRRGEADNRGSMWWLIQAGESEPVSPVPKVTAQM
                     LIKAIEQHGPQTADELALMFGITSRRANSSLAMAISKGRLIRVNQGGKFRYCIPGADL
                     PAEQEAASVTETDGKAFPLPAGVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDG
                     LILPSLHVANRELRRAKGQVQKWERVCAALRELNKQQDIVRQIVDSSGRIVSEK"
BASE COUNT          295 a          429 c          297 g          381 t
ORIGIN      
        1 cgccctgggt tgccagtttt gctatctcac gtagcgtctg ataattaatt tttctcactg
       61 gttgcctcct ggaaaataac tgcatgcccc agtttctccg ccagtgccag ttctgcctta
      121 gcgcctgccg accgctgcca gcctttcagc atgtaaatcg catccacaca acgaatcatt
      181 gccatgcaaa tatccatgta atgtggctga gtcagcccgt ccggaagtac tgccgggttt
      241 aaaacggtat gcccttcccg tttcagcgct tcttccgcct tgtgaaacgc ctcacggttg
      301 aaattttcat atcccgtcat cggaccagcg atataaattc tcaccctcac tccatcacct
      361 cctgaaaatt cccctgacag aacgccagta cacgctgcat gacttcgcta tttcggcact
      421 cacagcaaat tatgttctga tgcctgtcat agcggcgtat ttctccgtct ggtaactttc
      481 gaatcaatgt ctggtcagtt gttttctccg gtgtcttacg ccatacgcga tacacctgtt
      541 cagatgtaaa aacaccgtat ttaccggaca tgtataaatc gccacaagcc agtacatcca
      601 caaggcaacg tctgactgaa tgccagtctg ctcccgtcgc tctctccagt tgtgatatcg
      661 tcatgcgttc atttttgcgt accagcccga taattcgggc cttcagttct tcacgctgtt
      721 cgtgtgtaaa aggtttcgcc ataagcgcct ccggcaatca cttttccgac acaatacgac
      781 cggatgaatc gacaatctgt cgaacaatat cctgctgctt gttcagctcc cgcagcgcgg
      841 cgcagacacg ctcccacttc tgaacctgac cttttgcccg gcgcagctcg cggttagcca
      901 catgcagcga tggtaaaatc agaccatccg gatgttttct ggtgaacgac ggctgtgact
      961 gcactgtgac cgccacactt tccgttttta tttcttcctg tgtttccgct tcccggactg
     1021 gtaacgcaac acctgctggc agaggaaagg ctttaccatc ggtttccgtt accgatgcag
     1081 cttcctgctc tgccggtaaa tcagcgcccg gtatgcagta acgaaattta ccgccctgat
     1141 tcacgcgaat cagacgccct ttgctgattg ccatggccag cgatgaattc gcccggcggg
     1201 aggtaatccc gaacatcagt gccagctcat ccgccgtttg tgggccatgt tgttcaatcg
     1261 ccttaatcag catttgcgct gtcactttcg gtaccggtga cactggttca ctttcaccag
     1321 cctgaatcag ccaccacatc gaacccctgt tatccgcttc accacggcgc ttcagtttcc
     1381 acagttcgtt gaccgcatct tc
//
LOCUS       DAZFFI010000340         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFFI010000340 DAZFFI010000000
VERSION     DAZFFI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.343"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..515
                     /locus_tag="ACHJ12_005300"
     CDS             <1..515
                     /locus_tag="ACHJ12_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO4992911.1"
                     /translation="YSCESPVTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIG
                     SQEEENMWGNVYPRSGFVTQTDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGY
                     WPPDPVEENTGTRNHKWQRLTPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKR
                     GQTFLGSTNF"
     gene            529..>1397
                     /locus_tag="ACHJ12_005301"
     CDS             529..>1397
                     /locus_tag="ACHJ12_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO4992912.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKA"
BASE COUNT          348 a          314 c          451 g          284 t
ORIGIN      
        1 gatattcctg tgagtcaccg gtgacacctt ttgttcctta ttttctcagt acgcttgaca
       61 gtattgcctg gcgtacagga ctaccggagt cactgtaccc ggaggccctg atccccggta
      121 aacgcgaaat aggcagccag gaagaagaga atatgtgggg caatgtgtat ccccgctccg
      181 ggttcgtgac ccagacagat gatgataaag ctgctgcggt ggtggcccag cgggtggctg
      241 acattatcac ccgcaccggt gaaccacatg tctatcagcc ggtgaaagca gggaagaagg
      301 acggttactg gccaccggat ccggtggagg aaaatacggg cacccggaac cataaatggc
      361 agagactgac gccttcagtc tccacgtcct gcgcagtctt ccctgacgga gagcatgaag
      421 cggctgaaac aggtaatgct gcctttgccc tgtggcagcc ctacagttgc tgcaagaaac
      481 ggggacagac attcctgggc agtacgaatt tttaagtaag gggaagtgat gaaacagaaa
      541 atgacgatac ctgttctggc tgtggtgctt tgtgcgggta cggtacaggc tgacggaaac
      601 tttttgtccc tgccgaaagt gaatgacagt gcgtttggct acggtaaatc cgtggacggc
      661 gcagtatcag acaaactgtt ttataccctg ggagggggct cggtgatatc gcagccggca
      721 acgcgcagca atatgcagaa acttggggtg gatctcgggt ggagcagtga tctgatgtgt
      781 ggcaattttg acctgaaaac aacagtgggt aaccagctga acgggatcac cgatggtttc
      841 aaaaacctga tgggggatgt gattcagggg gcgagcggag ccgtggcgag tctgccggca
      901 atggtgatac agcgggctaa tccggggctg tatgacatgc tgaccaacgg tgtactgcag
      961 gcgaatgtgg cattcgacaa agcgcagctc aattgtcaga acatggcgaa acgcatgatg
     1021 gagttcacgg acagcaataa gtggacgcag gcggccatgc tcgaggagta caaaaaaatt
     1081 gtgaatacgg gggacggtga tgcgatcaaa ggggatgaag caggtcgtca ggttaccggg
     1141 gaggaggggc aacgctggat cggagggcag cagcgtggtg gagcgggaca gtctgctatc
     1201 cggccgacac atgatctgac agctgccggt ttcaatatga tgaacagtct gccttcgacc
     1261 agtacatcca gtgtcagtcc cgggaactgt aacgggagtg cctgcatgaa ataccgtaat
     1321 gcagaggaag ctgcccgggc ggtggtgaag gttctggggg ataaatccat gcggacctgt
     1381 ggtgatgcaa aggcatg
//
LOCUS       DAZFFI010000341         1377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFFI010000341 DAZFFI010000000
VERSION     DAZFFI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.344"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1025
                     /locus_tag="ACHJ12_005302"
     CDS             <1..1025
                     /locus_tag="ACHJ12_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4992913.1"
                     /translation="SQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSV
                     ADHGAGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQGTLSAEETKQ
                     LEEVKKERADMFATYIATHDAVSVGVGATTTGRHAVTRQIANVAAGSVDTDAVNVAQL
                     RDLQTVTEKADAALDKRITNNTSAITKNLQVVNSNISENSKQISTNTLGIRENTQRIR
                     DEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAGIAGAM
                     AMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDTENGAGVSA
                     GMALGW"
BASE COUNT          419 a          287 c          369 g          302 t
ORIGIN      
        1 attcacaggc aaatgcagaa aaaagtattt ctttaggtgc atcagcaaga gctattgaaa
       61 ataactctac cgcgttagga gcaaacgcaa caacattgaa accattctct gtcgctctgg
      121 gcgcaggaag tgttgctgat catggcgctg gtattgaagc atatatgcca gagacaaagc
      181 gctggaatcc aggggatgta gtaaaatcca ggatgtctga caagcagctt aacaggctta
      241 tggaaatcga taagctaata tccgcttata attcacaagg gacgctgtct gctgaagaga
      301 ctaaacaact ggaggaagtg aaaaaagagc gggctgatat gtttgcgaca tatatagcaa
      361 cgcacgatgc tgtatctgta ggtgttggtg caacgacaac aggacgacat gctgttacaa
      421 gacagatagc taacgttgca gcaggttctg tcgatacgga tgctgttaac gttgcacagc
      481 tcagggattt gcagactgtg acagaaaagg cggatgcagc gcttgataaa cgcataacaa
      541 acaacaccag tgcgataact aaaaatctgc aggtggttaa cagtaatatt tcggagaaca
      601 gcaaacaaat cagtaccaac actcttggta tcagagagaa tacacaacgg atccgggatg
      661 aaggacaggc tcgaatccgg ggagacaggg aaacactgcg tgcagcgacc ggttataccg
      721 acatgcgggt aaacgggctg gagcgtaata cgaataaacg ttttgcgcag cttaaagagg
      781 atatagacaa aaacagaaaa cgtgcagatg caggtattgc aggtgcgatg gcaatgacgg
      841 ggataccgtc cgtccccggg aaaaaggtct catttggtat ggccatggcc ggataccgga
      901 gtgaaggtgc tgttgctgcc ggcttccatt ttaatacaag tgaaaacagt gctgttaaag
      961 tgaatgcagc ctgggacact gaaaatggag ctggcgtttc agccgggatg gctctgggat
     1021 ggtaatgctt ccgggccaga agccgttcgc agatattgtt atccagggga cactgcacca
     1081 ccattcagga agcgattcag tttatcgcat cagctcagga tgtaattaat acggacaatc
     1141 tggctcctgc accggagcca gacgtacacg tgtcaacgcc acgatctttg acctgtatgg
     1201 ataaaagctc agattattta ccagcagcga catccggagc gcaccgggat tttatctgtg
     1261 agtgaacata cccgggcaaa aaaatccccc gaattcaggg gggtgtcaaa gaggaattat
     1321 gtatattggt tattataatg agcacgcctc cggtattccg cgacgaatgt tcacgtg
//
LOCUS       DAZFFI010000342         1368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFFI010000342 DAZFFI010000000
VERSION     DAZFFI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.345"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            53..586
                     /locus_tag="ACHJ12_005303"
     CDS             53..586
                     /locus_tag="ACHJ12_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4992914.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1105..1290
                     /locus_tag="ACHJ12_005304"
     CDS             1105..1290
                     /locus_tag="ACHJ12_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4992915.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          349 a          325 c          395 g          299 t
ORIGIN      
        1 gaaggcttaa catatccagg gattcggaac cgataaatcc tgataaatat ccatgaacac
       61 caaaatcaaa tacggcctgt cggctgccgt tctggcgctg attgccgcag gggcgcctgc
      121 gcctgatatt ctcgaccagt ttctggatga aaaagaaggt aaccacacca cggcataccg
      181 tgatggtgcg gggatctgga ccatttgtcg tggtgccacc agggtggatg gcaaacctgt
      241 cgtcccgggc atgaagttgt cgaagaaaaa atgcgaccag gttaacgcca ttgagcgtga
      301 taaggcgctg gcatgggtgg agaaaaacat ccgggtgcca ctgactgaac cccagaaagc
      361 ggggatcgcg tcattctgcc cgtataacat tggccccggt aagtgtttcc cgtcgacgtt
      421 ttataaacga attaatgcag gcgatcgccg gggagcgtgt gaagcgattc gctggtggat
      481 taaggacggt ggcagagact gccgtatccg ctcaaacaac tgttacggcc agatatcccg
      541 tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac agataagcag aatattttgc
      601 tgaaaaatga cgttggccaa cgcggacgga taacacgaaa tcctgcgaac tggcaaaacc
      661 taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag cggggttttg tgtttcctga
      721 cttcggaaaa gtcaaaggag aaagtgtgtt tgattttagc aaactgattc gggagattcg
      781 agtgatggct gaaaaattat ccacctggaa gttcatcctt atctggctgg tgtttgtgat
      841 tatggcctcc ggttatttca tcggtcagat acgctggtgg tgaaatgaac cgcgttctgt
      901 gtgtggtcat cattgccctg ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt
      961 accgcgataa cgccatcacc tacaaagcgc agcgcgataa aaaagccaga gagctggagc
     1021 tggcaaacgc aaccattact gatatgcagg tgcgccagcg cgatgttgct gcgctcgatg
     1081 caaaatactc gagggaatta gccgatgcga gagctgaaaa tgaaactctg cgtgctgatg
     1141 ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg ctccggtacc gtgcgtgaag
     1201 ccaccggcac ctccggcgtg gataatgcaa ccggcccccg actggcagac accgctgaac
     1261 gggattattt catcctcaga gaacggttga tgacaatgca gaagcagcag gaaggggcac
     1321 aggactatat ccgcactcag tgcctgaact aagttttgcg gatgcgcc
//
LOCUS       DAZFFI010000343         1367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFFI010000343 DAZFFI010000000
VERSION     DAZFFI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.346"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..1159
                     /gene="ompC"
                     /locus_tag="ACHJ12_005305"
     CDS             5..1159
                     /gene="ompC"
                     /locus_tag="ACHJ12_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4992916.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPASGKKVGEKAVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT          348 a          253 c          358 g          408 t
ORIGIN      
        1 aatcatgaaa aaaatgacag tggcactttc tgctgtagca gtggcagtga tgtttgctgc
       61 gggggcgcag gcagcagaag tttataataa agatggtaat aaactggatc tttacgggcg
      121 tgcaaccgct ctgcattact tctcggatga taaaggtaat gacggagacc agacttatgc
      181 tcgtctcggc tttaaaggcg aaacgcagat taatgatcaa ctgaccggat ttggtcagtg
      241 ggaataccag ttctctggta acaaaacgga atctgaaggt tccgcgggaa ataaaacccg
      301 tctggcattt gcaggtctga gatttgcaga tgttggtacc attgattacg gacgtaacta
      361 tggtattgct tacgatgtcg gatcatatac tgacgtactg cctgagtttg gtggtgatgg
      421 ctggacgcag accgataact ttatgacggc ccgaacttcc ggtgtcctga cttaccgtaa
      481 tacagacttc ttcggactgg ttgatggtct gaattttgcg gcgcagtatc agggcaaaaa
      541 tgagcgtgat gaccttcaga aggctaatgg cgacgggtat ggtttctcgg ccagctatga
      601 gtttgatggt tttggttttg tcgctgcgta taccaagtca gatcgtactg ataagcaggt
      661 tcaggggttg aatggtactg cagatgttct tgatcctgca tcaggtaaaa aggttggaga
      721 aaaagcagtt gactcaggta gtgtggcaaa aggtaaacat gctgaattct ggggaactgg
      781 tcttaaatat gatgccaata acctgtatct ggcagcagtg tattctgaaa cccagaatat
      841 gacgaccttt ggtgatcaag gtgttgcgga taaagctcag aatatcgaag ccgttgttca
      901 gtatcagttt gattttggtc tgcgcccgtc ccttgcctac cttcagtccc gcggacagga
      961 tgttatggtt ggtggtgtga accatggcga tcaggatctg gttaaatata ttgatgtcgg
     1021 tgcgacttat tactttaaca agaatatgtc cacctatgtt gattataaaa ttaacctgat
     1081 tgatgaaagt gaatttaccc ggaaagccgg tgttgcgaca gataatatcg ttgctgtggg
     1141 tatgacttat cagttctgat tattgctgat aagttaataa atacgggccg tctgccctta
     1201 caggcggcct gtatcaatga aaacacagtt ttcattggtc actgcgatca gcaattgcca
     1261 tctggcatgt gctgatttaa ctttctgtta ttacctttat tggttttatt ttaaattgga
     1321 cttttattgt tcggggcgcg tctgcgcccc ttttttatgg gcggata
//
LOCUS       DAZFFI010000344         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFFI010000344 DAZFFI010000000
VERSION     DAZFFI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.347"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1355
                     /locus_tag="ACHJ12_005306"
     CDS             <1..>1355
                     /locus_tag="ACHJ12_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4992917.1"
                     /translation="DQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVW
                     NGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGW
                     QTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGS
                     QGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGASTVNLING
                     SGKPVRVDITAHPAPDRIQVSALPDGVEAYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPVVQHLTVEVSASDGQYLALAKWDT
                     PRVVKGVRFSLRLTSGSGKNSRLVTSAITADTEHRFSGLPPGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAAPASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEKQITDILQTE
                     TS"
BASE COUNT          278 a          396 c          438 g          243 t
ORIGIN      
        1 gaccagacgg tcccggatgg tttcggggga acagagccgc ggatgacttt caatgcgtac
       61 ctgtcacagc agcgtaaggt gtgggatgtc ctgggggatt tctgctcggc gatgcgctgt
      121 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accgtccgtc ggatgtggtg
      181 tggccgtaca ccaacagcga tgtggtggtg gatgataacg gcgtggggtt ccgctacagc
      241 ttcagtgccc tgaaggaccg gcacacggcg gtggaggtga attacaccga cccgcagaac
      301 ggctggcaga cctccacgga actggtggaa gacccggacg ccatcctgcg ctacgggcgc
      361 aatctgctga agatggatgc gtttggctgt accagccgcg gtcaggccca ccgtgccgga
      421 ctgtgggtga taaagacaga actgctggaa acgcagacgg tggatttcac gctcgggtca
      481 caggggctgc gtcacacacc cggtgacatc attgaaatct gtgataacga ctacgccggg
      541 accctgaccg gcggacgcat cctgtccatc gatgccgcca gccgtaccct gacgctggac
      601 cgtgaggtga cactgccgga gacaggggca tcgacggtga acctgattaa cggcagcggt
      661 aagccggtgc gcgtggacat caccgcacac cccgcccctg accggataca ggtcagcgcc
      721 ctgccggatg gcgtggaggc atacggtgtg tggggactat ccctgccgtc actgcgtcgt
      781 cgcctgttcc gctgtgtttc catccgggaa aacacggacg gcacctttgc catcacggca
      841 gtgcagcacg taccggaaaa agaagccatt gtggataacg gggcccgctt tgagccgctg
      901 tccggctcac tgaacagcgt catcccgccg gtggtgcagc accttacggt agaggtgagc
      961 gcctcagacg gccagtatct ggcgctggcg aaatgggaca cgccgcgggt ggtgaagggc
     1021 gtgcgcttca gtctgcgcct gaccagtggc agtggtaaaa acagccgcct ggtgaccagc
     1081 gccatcactg ccgacacgga gcaccgtttc agtggcctgc cgcccgggga atacaccctg
     1141 acggtcaggg cgataaacag ctacggccag cagggcgaac ctgccaccac cacgttccgg
     1201 attaacgccc cggcagcacc ggccagcatt gaactgacgc cggggtatta tcagataacg
     1261 gcggtcccgc atcttacggt gtatgacccg acggtacagt ttgagttctg gttctcggaa
     1321 aagcagatta cggatatcct tcagactgaa acgtc
//
LOCUS       DAZFFI010000345         1337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFFI010000345 DAZFFI010000000
VERSION     DAZFFI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.348"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..378)
                     /gene="subA"
                     /locus_tag="ACHJ12_005307"
     CDS             complement(<1..378)
                     /gene="subA"
                     /locus_tag="ACHJ12_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO4992918.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSW"
BASE COUNT          414 a          284 c          290 g          349 t
ORIGIN      
        1 ccatgagtcc tgtacaccgt caggaataac ccttctgctg gatatcagtg catgaggata
       61 aacaccataa acctcatgac gtgaggcaat gagtgaagcc atggcagtac catgaatata
      121 taaagcttca gactctttta ccgggaatgg cgaaccatcc tgagtaaaac tgaatttggc
      181 aaattcgctg tcgctgaggc ctcctacaaa tgctactccg gaatcaacaa cagatacgac
      241 aacaggggta ttcttgtctg tgagcgacat ggttgtttct gtcaggccaa ttgcatcaaa
      301 ataccagggt ttttctgccc tgacagacac agatataaaa agcaggaaca gaatatgcgg
      361 ccataaaatc ttaagcacaa tatcctccag tcagtacaaa taaacagtat tgcaggtcaa
      421 tatcttacag gaacatcaca tctgaaatat cctgttttcg acaaaagata aacaatcaca
      481 accaataaag cgagtgaagc ccgtctcaaa aacattaatt tttatattta tgtatccaat
      541 gaattaacgc tcaccttcac ggtaatatgc atctggattt ctgcaacagg tcagcgataa
      601 gatgctctgc ctcctccttc agagcagaag taaatacagc catgatggat aaaacttaac
      661 cagaaaaaat aagaggaggc aagtaagttg cctggcatgt ttatgacaat aaaaagttcc
      721 gtcaggcatc atggcgaaca ggatatcaca gcgccgtttt accggggcaa tcagtgaatg
      781 gtggtgtggt tcattggtaa gccccggata tcaggtcact gagtatattc atgaatgacc
      841 gttaaaccgg ggatgcgagc tgctccccgg gaaagtcgaa ctgactggtc aatagatatc
      901 aatacggggc agtcagtcca cttacaggta tctgacaagg acaccttctg agcgtgcaat
      961 gtggacgggt aatacaacga tggtagcagg ctggtcagca atcagcagta tatgcccata
     1021 tttcctgagg ccggatatct gtttcctgag cttgttttcg acgtttgcta atattttgtc
     1081 gaactaatta tcagaacgat taatgataac agtgtggcag ggatctttac cgatatcaac
     1141 gtaaataatg accttaacag gtgcgtaatt gctggatttt gccatgctgt taccctgttg
     1201 atacgggtta attaaataac tttggggaag caggtaccgg cgttcactac cgctctccct
     1261 gataatgtat cctgcatgcc tgataacttt tgccgggcta atgtaaaata accctgaaat
     1321 atattaagtt gattaac
//
LOCUS       DAZFFI010000346         1334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFFI010000346 DAZFFI010000000
VERSION     DAZFFI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.349"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..267)
                     /locus_tag="ACHJ12_005308"
     CDS             complement(<1..267)
                     /locus_tag="ACHJ12_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992919.1"
                     /translation="MGLFNYKDYHQAQITGLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSP"
     gene            complement(326..>1334)
                     /locus_tag="ACHJ12_005309"
     CDS             complement(326..>1334)
                     /locus_tag="ACHJ12_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992920.1"
                     /translation="AFYERALPSNVSGDLYPQPSIFGDKVSSVSKNWSTLLDSNPGSY
                     VTSQRLDSGANQYNYNGHTGSDVISITDSFGGLDRTQVSRFPVGLFTGEGNDLIVTGR
                     DYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVTLYVNEEGQLRATTDSYNGSTSI
                     DYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGNSADAIINTNIDLGAGNDFIYAN
                     GEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSGFEHVDLHSTSHLILNSDDFKSQ
                     DIEGEILKISGSSGASVDVQNFDWENLSSANDGDVKYFTYQSTDIPGLTLWIQEGIEV
                     K"
BASE COUNT          414 a          300 c          217 g          403 t
ORIGIN      
        1 cggactggga aagcgataaa aacctcttga atcgacatat tccgaagata acctgagttc
       61 ttccactgta atatcacgcc agccttcatt aagaacatcg gtagaataat caatcctatc
      121 tgggtgcttt gcaaatagcc tggataccag aaaacttcct aatccaagaa cctttcctgt
      181 attggcatat actgcaatat tataagacgc agagatcagg cctgttattt gcgcttgatg
      241 ataatcttta taattaaaga gtcccatgag tatttcctta tttaaagaac agccccatgc
      301 tggggctgtt tgtttagatt ttcttttatt taacctcaat accctcctgt atccaaagag
      361 ttaaaccagg aatatcagtg gactgatagg tgaaatattt aacatcacca tcattggcac
      421 tacttaaatt ctcccagtcg aagttttgta catccacgga tgcacctgaa ctacctgaga
      481 tttttaatat ctccccctct atatcctgag atttaaaatc atctgaattc agaatgagat
      541 gagatgtaga gtgcaaatca acatgctcaa aaccagagaa catagcactt gttactgtag
      601 tattgtccaa agatatcgtg tcaaagccct ctccacctat aatatttacc tgagtaccat
      661 tgttagttga aatctctcca ttagcgtaaa tgaagtcatt acctgccccc aagtctatat
      721 tcgtattaat aatagcatcc gcactattac cttcataccc taaagccaga acagtatcat
      781 ttcctgcccc cataacaata tcagttcctg atattgttcc acctgatgat gaagaattga
      841 gcccagtata gtcaatggat gtactaccat tataactatc agtagttgct ctcagttgtc
      901 cttcttcatt cacatatagt gttacatcgt tatttccgac accaacaacc aaggtatcat
      961 ctccattccc catatcagta cgatgagagt gatccgtgta tcctgcactg gtattacgtc
     1021 cataatctct tcctgtaacg attaaatcat tcccttctcc tgtgaataaa ccaaccggga
     1081 agcgagatac ttgagttcta tctaatcctc cgaaagaatc ggtaattgag ataacatcag
     1141 aacctgtatg accattgtaa ttatattgat tagctcctga atctaatctc tgggatgtaa
     1201 cataacttcc aggattacta tcaagtaaag tagaccagtt cttagaaaca gaagatacct
     1261 tatcaccaaa tatggacggt tgaggatata agtctcctga tacattagag ggcaatgccc
     1321 gttcgtaaaa tgcc
//
LOCUS       DAZFFI010000347         1330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFFI010000347 DAZFFI010000000
VERSION     DAZFFI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.350"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHJ12_005310"
     CDS             <1..552
                     /locus_tag="ACHJ12_005310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4992921.1"
                     /translation="RAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQAL
                     FARVAEEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVW
                     THKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATG
                     VELETERFEQTVREVQDLVSQNG"
     gene            1050..>1330
                     /locus_tag="ACHJ12_005311"
     CDS             1050..>1330
                     /locus_tag="ACHJ12_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4992922.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVTTPVVVTGDDGKEVLISATSEPEYYYVITLAGQSNSMA"
BASE COUNT          333 a          309 c          387 g          301 t
ORIGIN      
        1 cgcgctgccc tggggataac ggcaaaacag attgtggatg ccacaggaaa gaaaaacatg
       61 gtgtcgcact ggttcagtgc cagtcagtgg cagctgccga acgaaagtga ttatctgaaa
      121 ttacaggcgc tgtttgcccg ggtggcagaa gagaagcatc agcgcggaga actggaaaag
      181 ccacatcacc aactggtcag cacatacagt gaactgaacc ggcagtatac ggaactgcag
      241 agtgaatata agcatctgcg gcggtatttt ggcgtgacgg cgcaggtgcc gtacacggat
      301 gtgtggacgc ataaaccggt gcagtactat cccgggaaac atccgtgcga aaaaccggca
      361 gaaatgctgc agcagataat cagcgcgagc agtcgtccgg gtgacctggt tgcagatttt
      421 ttcatggggt cgggttcgac agtcaaagcc gcgatggcgc tggggcgtcg tgcaacaggc
      481 gttgagctgg agactgaacg ttttgagcag acggtcaggg aagtacagga tttagtcagt
      541 cagaacggat gatattgcag aattaaccgg gtatcagtta cgccccgaaa attttaaatg
      601 tctcacaatt cagacggttg acagttgtct gatttgcggg gagtttgtta aaagaaacag
      661 gcatggtgaa tccccctgag cggaggggca tatcagcgac aggtgtttgg ttacatccct
      721 tatccttatc cagcgcgggt tcaggtgctg atactgaact caccgggagg cacccggcac
      781 catgcgcatg atgatacaga tacgcggctt tagcccctct ccggaggggc tttcttgtag
      841 gcaaaaaaag cccgagtggg ttcgggcaac agcatgagat atacattttt ataatcgaat
      901 ggattttaac cagaattcat aaggctgcgc aactgcgtgg cctttttcgt attgcgggct
      961 gtagtcttcc tcctgtcatt gtcctgtaac ttccggactt cagcccgctc cttatccgac
     1021 taacaacatt atcccgaccg ggaggattca tgacatttaa acactacgat gtggtcaggg
     1081 cggcatcgcc gtcagacctt gcggatgcgc ttgctcaaaa aattcgtgaa ggatggcagc
     1141 catacggcgg gccgttttct tcgtatacgg atgatggcgc agcacttatt caggcgattg
     1201 tcgctgaagg tgatgtcacc acgccggtgg tggtaaccgg tgatgatggt aaggaggtgc
     1261 ttatcagcgc caccagcgaa ccggagtatt actacgtgat tacgctggct ggccagtcaa
     1321 acagcatggc
//
LOCUS       DAZFFI010000348         1328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFFI010000348 DAZFFI010000000
VERSION     DAZFFI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.351"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>1328)
                     /gene="ehxB"
                     /locus_tag="ACHJ12_005312"
                     /gene_synonym="hlyB"
     CDS             complement(<1..>1328)
                     /gene="ehxB"
                     /locus_tag="ACHJ12_005312"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO4992923.1"
                     /translation="VTEWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYIL
                     SRVTKDSSRYLVYDPEQHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFI
                     PSVVKYRRILLEVLTVSAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVI
                     LFEVILTGARTYIFSHTTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQ
                     IRNFLTGQALTSVLDLFFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRR
                     LDDKFLRNAENQAFLVETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMT
                     GQQGIQLIQKSVMVISLWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDF
                     QQVGISVERLGDVLNTPVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVI"
BASE COUNT          448 a          293 c          218 g          369 t
ORIGIN      
        1 aataacatta ccgtcagaag aataccgaaa tctgacattt ttaaattcga tatccccctg
       61 aatttccggc agtatatttc tgcctgactt tttctctacc ggtgtattta atacatcccc
      121 aaggcgctca acagaaatac caacttgctg aaaatcctgc caaagatgag ccagtctgat
      181 aacaggagca atgacctgac ctgcaagcat attaaacgcg attaactgcc cgatgcttat
      241 ctctcccgat atgacaagat gtgctcccat ccatagacta attaccatta cacttttctg
      301 tatcagctgt atcccctgct gccctgtcat tgcaaccaga tttaccctga aactggaagc
      361 aacatacccg gccagttgtt tatcccatgt agcaatcatc tgtggcgata ctgccatgga
      421 tttaattgta tttattgctg ttaccgtttc gacaagaaaa gcttgatttt ctgcattcct
      481 gagaaactta tcatcaagac gtcgacgtaa taagggtgat ataaacaatg accatataac
      541 ataacaaggt agtgacaata atataaccag tgttaattga ggactgtaat accacatgac
      601 acaaaaaaat attacagaaa aaaacagatc aagaactgat gtcaacgctt gtccggttaa
      661 aaaattacga atttgctcca gttcccttac tctggcaacg gtctctccga ccctgcgatt
      721 ttcaaaatat gaaacaggca atgcaagcaa atgtctgaat aacttagcac ccagttcgac
      781 gtcaatacga cttgtagtat gagagaaaat ataagttctg gctccggtta atatcacttc
      841 aaaaagtatc actataataa atgctattgt gataatattt aacgttgaaa acccccggtg
      901 aactaaaacc ttatccatta caacctgaaa aaaaagaggt gttattaacg caagaaactg
      961 aataaaagca gaaacagtta acacctcaag taaaatcctc ctgtatttca caacagaggg
     1021 gataaaccag gaaaaatcaa atttagctaa ctctccgact actgttgctc ttgacgtaac
     1081 cagaatgact tttccctgat acagtttttc gaactcatcc cgactaaaag ttagtgactg
     1141 atgctgttct ggatcataaa caagatagcg tgatgaatct ttagtaacac gagataatat
     1201 ataatgctta ccgtcatccc gccatatcaa cgcaggtaaa gaaattattg acaatctgga
     1261 aaaatgtttt tctacatatt ttgcttttaa gccaatggat ttcgctgcca gtaaccattc
     1321 agtcaccc
//
LOCUS       DAZFFI010000349         1327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFFI010000349 DAZFFI010000000
VERSION     DAZFFI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.352"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1327
                     /gene="ygeH"
                     /locus_tag="ACHJ12_005313"
     CDS             <1..>1327
                     /gene="ygeH"
                     /locus_tag="ACHJ12_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO4992924.1"
                     /translation="YHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLLESAGHVVL
                     KDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKI
                     NEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHI
                     SQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFI
                     IDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTIFDRL
                     QNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILA
                     IMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEARICIDKSLQ
                     LEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKL"
BASE COUNT          477 a          235 c          227 g          388 t
ORIGIN      
        1 catatcattt tcttgaggga ttaacgctca cggaagatgg aattcttact caaggaaatg
       61 agcaagttta tattccacag aaagagttag gtgtattaat agtattactt gaatccgctg
      121 gtcatgttgt actgaaagat atgatcatcg aatcagtatg gaaaaatatt attgttagtg
      181 acgagtccct gacaagatgt atctattctt tgcgctgcat ttttgaaaaa attggctatg
      241 atcgttgcat agaaacaatc taccggaaag gttatcgttt cagcgggcag gttttcaaaa
      301 ctaaaattaa tgaagataat acttcagact attccatagc tatattccct ttcactactt
      361 cattgaatac actggatcca ttaatactta atcaggaatt agtgcaaatc atttcaaata
      421 aaaaaatcga tggtctctat acctatccga tggctgcgac aaatttttgt aatgatcaca
      481 tatctcaaaa ttcattcttg agcagattca agccagatta tttcgttaca ggaagaataa
      541 accagaataa tgcagtgaac actttataca ttgagttgat cgacgctaaa aaccttttcc
      601 tcatcgccag taatcatctc cctgttgatg aactacataa tacatcacaa tttattatag
      661 ataatatcct tcaaacggta cataaaccag aacgatctgt aagattagct aagcaggacc
      721 aaggatataa gaatcattat ttatcagatg aaatgttagc cggaaagaaa gaactttacg
      781 acttcacccc tgaaagcatt tacagggcca tgactatatt tgatagatta caaaataaaa
      841 gtgatataca gacgctaaaa acagaatgtt attgccttct agcggaatgc catatgtctt
      901 tggcacttca tggaaaaagt gaacttgaac ttgctgctca aaaagcatta gagcttttag
      961 attatgtatc agacataacc actgtcgatg gaaaaatttt agctattatg ggactgataa
     1021 ctggtctgtc tggacaagca aaagtatctc atatcttatt tgaacaggct aagatacacc
     1081 caactgatat agcctctctc tactactata gggcactagt ccactttcat aatgaaaaaa
     1141 ttgaagaggc aaggatttgt atagacaaat cactacaact cgaacccaga agacgaaaag
     1201 cagttgtgat aaaagaatgt gtagatatgt atgtgcctaa cccgctcaaa aacaacatta
     1261 aactctacta taaagaaact gagagtgaaa gccatcgagt tataattgac aacattttga
     1321 aattaaa
//
LOCUS       DAZFFI010000350         1320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFFI010000350 DAZFFI010000000
VERSION     DAZFFI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.353"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..739
                     /locus_tag="ACHJ12_005314"
     CDS             5..739
                     /locus_tag="ACHJ12_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4992925.1"
                     /translation="MKNIYKIIACAVIGTSMVAIPAHAAEGDHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHSRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVTADLKIKDNISSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            971..1108
                     /locus_tag="ACHJ12_005315"
     CDS             971..1108
                     /locus_tag="ACHJ12_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4992926.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     K"
BASE COUNT          346 a          256 c          355 g          363 t
ORIGIN      
        1 aaaaatgaaa aatatttata agattattgc gtgtgcagtt attgggacga gcatggtagc
       61 cataccagca catgcagcag aaggagacca tacaatttct ctggggtatg cgcactttca
      121 gtttccggga ctgaaggatt ttgtaaagga tgcgactgct cataacaggg agactttcag
      181 tcatttcgtc aacagaaact acttttcttc attgggcgaa tatacagatg gtcgggtcag
      241 tggatatgaa ggcaaggata aaaatccaca gggcattaat atcaggtatc gctacgagat
      301 aacggatgat tttggcgtta tcacctcttt tacatggacg cgttctctca ctaactcaca
      361 gacatttatt gatgtgcagt cagccgatca tagcaggaag attaagaatc cggcagcttc
      421 tgccagaacg gatatcaggg cgaattactg gagtctgtta gcggggcctt catggcgggt
      481 taatcagtac atgagtttat atgcgatggc agggatgggc gttgctaaag ttaccgctga
      541 cctgaaaatt aaggacaata ttagcagtag tggcggattt tctgaaagca acagcacgaa
      601 aaaaaactcc cttgcgtggg ctgcaggtgc acagtttaac ctgaatgaga gtgttacact
      661 ggatgtggct tacgaaggtt ccggctctgg cgactggcgc acgagtggag ttactgctgg
      721 cattggcctg aaattctgat ctgtatccgg taaccgttta ctacccgctg tgatggcggg
      781 ttttttattg cccgtacagg gcaaaaaccg taaattatgc gtgggtgcct ttcggctgat
      841 ggctggaggg agaacctgaa ggcctgatgt ggaaaggccc cgagtcaact taacgttaac
      901 ccgaggccct aaccatcata ccttaagcaa gtagaaggtt agcgcctctc tgtaaaagga
      961 gtcaagcgct atgtcgcaaa aatcgcttat caccgtcaca atttgtatga cggttatctt
     1021 caccatctgg atgttacacg gctcgttgtg tgagttccgg ctgaatttgt ggggagcgga
     1081 gtttgcggcg ttcttacagt gtaagtagta ggaaaaccgc gacggggacg aaagtccccg
     1141 tcaactggtt gctgaggttc agtcgatatg gcacccgttt gtagagtgtc tttcatccaa
     1201 tcccggacag gtcgagagag gggatatgtg tgaatgggaa ataataacta ttggaaatgt
     1261 tttgtttttt ctaagtcttt ttggcggtgt gtatataaat ttttgatgtg ttccgtattt
//
LOCUS       DAZFFI010000351         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFFI010000351 DAZFFI010000000
VERSION     DAZFFI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.354"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1241
                     /gene="ehxA"
                     /locus_tag="ACHJ12_005316"
     CDS             <1..1241
                     /gene="ehxA"
                     /locus_tag="ACHJ12_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4992927.1"
                     /translation="EKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHDTVSYNK
                     TDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFR
                     TGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDD
                     RDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDK
                     LYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLSDISFDD
                     IAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGR
                     EIKADKIPHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDSGDERLAS
                     LYNLSLHQNNTHSTTLTTTV"
BASE COUNT          447 a          170 c          322 g          380 t
ORIGIN      
        1 ttgaaaagga taacaaacac tatcaggcga gaataatttc tgagctcgga gataaagacg
       61 atatagttta ttctggggca ggctcatcag aagtatttgc tggtgaaggt catgataccg
      121 tatcttataa taagacggat gttggtaaac taacaattga tgcaacagga gcatcaaaac
      181 ctggtgaata tatagtttca aaaaatatgt atggtgacgt gaaggtattg caggaagtcg
      241 ttaaggaaca ggaggtgtca gtagggaagc gaacagagaa aatacaatat cgtgattttg
      301 aattcagaac cggtggaatt ccttatgatg tgatagataa tcttcattct gttgaagaac
      361 tcattggcgg aaaacatgat gatgaattca aaggcggtaa gtttaatgat atattccatg
      421 gcgcagatgg gaacgattat atcgaaggta attatggtaa tgatcgacta tacggcgatg
      481 atagggatga ttatatatcc ggaggacagg gagacgacca gttatttggt ggtagtggaa
      541 acgataaatt gagtggaggg gatggtaata attatctgac aggaggaagc ggtaatgatg
      601 agcttcaggc acacggagct tataatattc tgtcaggtgg tactggtgat gataaacttt
      661 atggtggtgg tggtattgac cttctggatg gaggggaagg taatgactat ctgaatggtg
      721 gttttggtaa tgatatttat gtttataggc aaaactatgg tcatcataca attgcagatg
      781 aaggaggtaa aggagatcgt ctgcacttat ctgatattag ctttgatgat atcgcattta
      841 agagagttgg aaatgatctt atcatgaata aagccattaa tggtgtactt tcatttaatg
      901 agtcaaatga tgtcaatggg ataacattta aaaactggtt tgcgaaagat gcctcaggag
      961 cagataatca tcttgttgag gttataacag ataaagatgg tcgtgagata aaagctgata
     1021 agatacctca taataataat gaacggtcag gttatataaa agccagtaat atagcatctg
     1081 aaaaaaacat ggttaatatc accagtgttg ccgatgatat taacaagatt atttcttcag
     1141 tttcagggtt cgattcaggt gatgaacgat tagcatcttt atataattta tccttacatc
     1201 aaaacaacac acattcaaca actttaacga caactgtctg agactaaaag cggaggtgat
     1261 ggccattcca ggaggaatgg tcatcgttat ataatgagta aatgtagttc tcataatag
//
LOCUS       DAZFFI010000352         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFFI010000352 DAZFFI010000000
VERSION     DAZFFI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.355"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..>1309
                     /locus_tag="ACHJ12_005317"
     CDS             63..>1309
                     /locus_tag="ACHJ12_005317"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4992928.1"
                     /translation="MNIRNNHLPGTETLFSETETANSKQLAVLKTNFPQCFDKNGAFI
                     QEKLLEIIRASNVEISKESYCLNWLGKSYARLLANLPPTTLLSEDKDHNRREENKNSQ
                     NLLIKGDNLEVLKHMVNAYSEKVKMIYIDPPYNTGSDGFAYNDDRKFTPEQLSDLAGI
                     DLDEAKRILEFTTNGSSSHSAWLTFIYPRLYIARELLREDGVIFISIDENELNQLKTI
                     CDEIFGEANFIENIVWNKRIPKNGKGIGNIHEYILAYAKNNEISKEFLMRKDGIDDVY
                     QFIEKLKKEKVPLDKAEQQLKKFYSSNGYDRGITLYNGLNEDYRPWGKINMSWPNADS
                     FGPTYEVLHPLTNNPVKIPDRGWRWKEGTFNHIAKRIDGKYADIKKLHDGSVICGGIW
                     FARTEKTQPSSVKFLDEVEEFLL"
BASE COUNT          440 a          227 c          266 g          376 t
ORIGIN      
        1 atatgctcgc ctgaactctc tccgtcaagt acctctacct ccgtgctttc tggaggtgct
       61 gtatgaatat acgcaacaat catctaccgg gaacagaaac tcttttttct gaaactgaaa
      121 ctgcgaacag taaacaactt gctgttttga aaactaactt tccacagtgt ttcgataaaa
      181 acggagcttt cattcaggaa aagctgcttg agatcatcag agcatcaaat gttgaaatct
      241 ctaaagagtc atactgctta aactggcttg gcaaatccta tgctcgcctg ctggctaacc
      301 taccaccaac gacattgctg tcagaagata aagaccataa ccgacgtgaa gagaacaaga
      361 acagtcaaaa tctgttaatc aaaggggata accttgaggt attaaagcat atggttaatg
      421 cttattctga aaaggttaaa atgatttata tcgatccgcc atataacacg ggttctgatg
      481 ggttcgccta taatgatgat cgcaaattta caccagaaca gctttctgat ctggcgggta
      541 tcgatcttga tgaggctaaa cgtatacttg aatttaccac gaatggttca agcagccata
      601 gcgcatggtt aacgttcatt tatcctcgtt tatatattgc tcgtgagctt ctacgtgaag
      661 atggtgtgat atttatttcg attgacgaaa atgaattaaa tcaacttaaa actatttgtg
      721 atgaaatttt tggcgaagca aatttcatag aaaatattgt atggaataaa cgtatcccta
      781 aaaatggtaa gggtattggt aatatccatg agtacatcct tgcttatgcg aagaataacg
      841 aaataagtaa agaattttta atgcggaaag atggtattga tgatgtatat cagtttattg
      901 aaaagttaaa aaaggaaaag gtgccattag ataaagcaga gcagcaactc aaaaaattct
      961 acagctccaa cggatatgat cgtggaatta ctctttacaa cggtttgaac gaggattata
     1021 ggccatgggg taaaattaat atgagttggc ctaatgcaga ttcatttgga cccacgtatg
     1081 aagtattgca ccctttgact aataaccctg tcaaaatccc agacagaggc tggcgttgga
     1141 aagaaggcac ttttaatcat attgcaaaaa gaatagatgg aaagtatgca gatatcaaaa
     1201 aactacatga tggtagcgtt atttgtggtg gaatttggtt tgcccgtaca gaaaaaaccc
     1261 aacccagttc ggttaaattt ttagatgaag ttgaagagtt tttactaag
//
LOCUS       DAZFFI010000353         1302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFFI010000353 DAZFFI010000000
VERSION     DAZFFI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.356"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..655)
                     /locus_tag="ACHJ12_005318"
     CDS             complement(<1..655)
                     /locus_tag="ACHJ12_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4992929.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     "
     gene            complement(1174..>1302)
                     /locus_tag="ACHJ12_005319"
     CDS             complement(1174..>1302)
                     /locus_tag="ACHJ12_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4992930.1"
                     /translation="NESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRWLARNTN"
BASE COUNT          326 a          325 c          317 g          334 t
ORIGIN      
        1 tcattttgtc gggagacggg gcgttattct ggcgcagttc tttctccatg cgatcgaact
       61 cagcgatgta ggcttcttta aatgcagcgg cttttttgcc ggtgaagccc atcaccagga
      121 aaacgaagcc gtttttggtt atttggtaca tagggagttt gcgaccggtt gagtcggtat
      181 attcgctcga cacaaaattg tgctcagtga attttgctga acagtccaga ttgcgaattt
      241 tatccaacac tcgttcgtgg cgtttaccaa agaactcagc gatcgcaata gacgtagtga
      301 cagcgcgtcc attttcgatg gttacgtcag ggtgagaaag ggcagggata gtagccatga
      361 tggcagcctc gatagtcagt ttaaataact caccaccaag gctttccacg accataaggg
      421 tggtgagacg tacaggggtg gaaataccgg tgactaccga aaccggccag cctttcggct
      481 gccctgcacg ccccaccatg atctgaatgt ggctgtgcat tacgcataaa aaaaccgcct
      541 gagcgcggtt atgcgcagta gtcatcatcg ggtttccacg cccggcaccc gctttatgag
      601 gtgcaggtgc actataattc cacccgttct ggttttcaat agctacattc aacattttct
      661 cttacccttc atcaccgaag tgaactttgt tgatgcagtg cctggtgcct ccaggtgacg
      721 ttaaccagtt aacaattaac gccggaatac agaaggatgc ccgttacgcc ccgtaaaaga
      781 ccactttacg gttttaattg ttccgcgtgc gcatagccgc attcaccgca tcacaaaatt
      841 cactttaaaa agggcggaca tcagaaagct aataaactga tgccgccaaa ggtaccactg
      901 ccattgaaac atggagatgt tgtggcgggg ttgtcactca ggcatatggt caacctgaca
      961 acccggtgtc ctcaatgagg ggaaaggaaa taaaaacccc cagccatatt taccgccgcg
     1021 ccatttcgcg gattgccaca accggaagca cacggtcgaa gaaatctaac gacaagcctt
     1081 ctaaggaaaa gacgccttcg ccgtgtgctt tctgttgtgc cctgactttt cagggtaaat
     1141 taaccagtgg caacctgttt ttactggtgc taatcagtta gtgtttctgg ctaaccagcg
     1201 acgcgcgcca gcttcggttt taaacttttt acttttggta tacgtcatcg cggtaaacgt
     1261 gccgtcctgg ttagggaaca caccgcatac cagtgattcg tt
//
LOCUS       DAZFFI010000354         1286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFFI010000354 DAZFFI010000000
VERSION     DAZFFI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.357"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(30..257)
                     /gene="mchB"
                     /locus_tag="ACHJ12_005320"
     CDS             complement(30..257)
                     /gene="mchB"
                     /locus_tag="ACHJ12_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO4992931.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(285..494)
                     /gene="mchI"
                     /locus_tag="ACHJ12_005321"
     CDS             complement(285..494)
                     /gene="mchI"
                     /locus_tag="ACHJ12_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO4992932.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            603..839
                     /locus_tag="ACHJ12_005322"
     CDS             603..839
                     /locus_tag="ACHJ12_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992933.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
BASE COUNT          441 a          268 c          231 g          346 t
ORIGIN      
        1 aagtgcctgt aaaataaata aaaatataat tagctaccgc caccagcaga agaactggca
       61 ctaccacttc ccacggtcga gccaattgct gttgtcaaac cggcagatac ggctccaact
      121 acaaccccaa gtggaccacc aggtattctg gcgagagctc caacaatagc tgcagcacct
      181 gcagcattag ctgaagtcgc tggcgcacct cccgccccgg aaatatatct taactgtgat
      241 tctgttattt ctcgcatata aactgcatat aaactccatc atatttaact tccgtttttc
      301 cagcctgacc agaccagaat ccttcgaatc ccatgaatca gctcaataaa accaagaaat
      361 attataaagc ttagaaaaac gtcaacataa gaattccctt cgccaacaat ccgaagggat
      421 gaaagtgaaa ccaataagat agtaataggt aaactgaata tagccgtcaa ttgggacagt
      481 tttttataac tcattaatcc ctacctgttg ctttattttc attatgaaaa gttaccaaat
      541 cttagcacct cagtcaggag agtcaggtca tcgataaaga atattccagt agagtggcgc
      601 tcatgatcaa agataacagc aaagttgatg acagcgctga ccgactgtca tttacagaaa
      661 ccctgtttgt agcacattcc atcttacctg acctcaccca gacaaagaca aaagagaatg
      721 attgttattt acttttcaaa aaaaagtgtc atattgtcca tataaagtca agaggtattt
      781 tacgttatgt cactgatgca attgttagtc aggatctcat ggttgtcgct catgtataac
      841 ctgagacttg aacgcacact aatagcactg acggtcatac atcgggaaat gtgaggaggc
      901 ataagaatga attgtgataa taatcacaga aatgaagaat tcattgttac ctttgataaa
      961 ggcaacaagc aagacaattc aagacgaaaa cacgataatt ttcctataga ggtagaatcc
     1021 tccgtagagc aggagacaca ctgtatcaca aataataagt cggcttccgg tatagtaaca
     1081 catgactatg atgccgatta tatttgtggt tgtggtgaaa ttatgtgtcc tggttgcggt
     1141 catgacctat aacaaacatt attgcaaact gatacctgat caggtacaag cctgttgatt
     1201 attatgctga atatttcatg ttataaaacc atcgatataa acattaacaa gaaaaaacat
     1261 atattgttta tctgaacatt tatcga
//
LOCUS       DAZFFI010000355         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFFI010000355 DAZFFI010000000
VERSION     DAZFFI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.358"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..66
                     /locus_tag="ACHJ12_005323"
     CDS             <1..66
                     /locus_tag="ACHJ12_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4992934.1"
                     /translation="CYGQVSRRDQESALACWGIDR"
     gene            223..407
                     /locus_tag="ACHJ12_005324"
                     /pseudo
     CDS             223..407
                     /locus_tag="ACHJ12_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            422..553
                     /locus_tag="ACHJ12_005325"
     CDS             422..553
                     /locus_tag="ACHJ12_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992935.1"
                     /translation="MSDKLIMLAKILCVIIGISFSLMLVALFLSMGWMMLSSSGMLG"
     gene            776..982
                     /locus_tag="ACHJ12_005326"
     CDS             776..982
                     /locus_tag="ACHJ12_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4992936.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1047..>1275
                     /locus_tag="ACHJ12_005327"
     CDS             1047..>1275
                     /locus_tag="ACHJ12_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992937.1"
                     /translation="MQKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVEYTMNAAKCRMVSAALTETAAR"
BASE COUNT          324 a          281 c          349 g          321 t
ORIGIN      
        1 tgttatggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggtatcgac
       61 agataagcag aatattttgc tgaaaaatga cgttggccaa cgcggacgga taacacgaaa
      121 tcctgcgaac tggcaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag
      181 cggggttttg tgtttctgac cttggataag gcaagggaga acatggaaaa gtataaacga
      241 attctgttga ggttgactat gaaaaacggc cttgaactga aagcgccagt aactgatgac
      301 atcagcagag cactggcttt tgctattaag tgggtggcgg tcggtatatc gctgtgtctc
      361 cgatgctgta tgggctggca aaactggtca ttgcgttgaa atcgtgaagg gaggattaaa
      421 catgtcagac aaactcataa tgctggcgaa gatcctctgt gtaatcatcg gcatttcatt
      481 ttcactaatg ctggttgctc tttttctttc catgggctgg atgatgttat cttcgtcggg
      541 gatgctgggg tgagcataaa ccgaatgctt tccgcgttta ccgttattct gctggtggtc
      601 tgtggtgcgc ttagtctggg gctgaatcat taccgcgata acgccatcac ctacaaagcg
      661 cagcgtgata aaaaagccag agagctggag ctggcaaacg caaccattac tgatatgcag
      721 gtgcgccagc gcgatgttgc tgcgctcgat gcaaaatact cgagggaatt agccgatgcg
      781 agagctgaaa atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg cctgcggatc
      841 aacgccacct gccccggtac cgtgcgtgaa gccaccggca cctccggcgt gggcaatgat
      901 gccgccgtcg aactctctcc ggttgctgga cgaaacgttc tcggtatcag agacggaatc
      961 atcagcgacc aggcagcatt gagaatgctt caggaatata tccgcactca gtgtattaac
     1021 tagtattttt gttattcgga gaatgcatgc agaaattatt ggtaaccgta aagccttttc
     1081 agggaacaat tccgttccgt attttgcagc gtggtcgtgt tcttgttgaa ggttcgttca
     1141 gtggtaaatg tacgcaatta cactcccgga cctttcaggt gaatgccacg aatgaagagc
     1201 taaccgttga gtatacgatg aatgccgcta aatgccgcat ggtatccgct gcattaacag
     1261 agaccgcagc ccgac
//
LOCUS       DAZFFI010000356         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFFI010000356 DAZFFI010000000
VERSION     DAZFFI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.359"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(139..964)
                     /locus_tag="ACHJ12_005328"
                     /pseudo
     CDS             complement(139..964)
                     /locus_tag="ACHJ12_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          305 a          330 c          286 g          352 t
ORIGIN      
        1 aaaattaatg tattccagag gtttcacatt agtctccaat ttttcagaag aatctattta
       61 cctggcagga aaagtcatct ggtgtttcgc cggagtgagg ggtacaggca atagcggaca
      121 taaccatttg ttctttttct aagcagccat ctgataatat tttatcctga aggctgtcgg
      181 ggagatattc cccagacgag agtgacgacg ctgacgattg tagaaaatct caatgtattc
      241 ccgtattact gagatggcct catcccggct tttaaaacga tagtggctca gactttcatt
      301 tttcagcgtt ccccagaagc tttccatagg agcattgtcg taacagttaa ctttacgcga
      361 cattgatatt ttcagaccaa actgctcctg tatggccctg taatcgtatg cgcagtactg
      421 tgaaccacga tcggagtggt ggattagccc ggtaggtgag cgctggttcc tgagcgccat
      481 aaacagggct ttacccgtca actatttcgt catgcgctct cccatggcgt aaccgacaat
      541 tttgctcgta taaacatctt tgacgccagc gatatacagc catccttccg gggggcaaca
      601 tacgttatat ctgcaaccaa gacctgatta ggtgctgtag gcgtaaagtt ctgattcagg
      661 agattcggcg caaccggcag attgtgacaa gagttcgtgt tcgccctgaa cttgcgtttc
      721 tgcttacagt gcagcctcag ctccttacga agacgtgcca gtctgtcacg accgacgata
      781 attccgttat ctgccagctc cgtctggagc agctaggttc catatgtttc gcgtgtgcgg
      841 atatgtgcca ccttaatctc cagttttagc cgctcatcac tttgttttct gtctgagggt
      901 catgctgtac tcagtcgtaa taaccgatcc tgtatacacc aagtacccga cacatcgctt
      961 caatacataa tcgcatctga atcgttcacc gattcgcggc atttttcaag gtagttgtcg
     1021 ccattgttcg actaactgat cgacattaca gcctagagcc tagctggggg ctgtgcacaa
     1081 aaaagccccg gacattccgg ggctacactg catattaacg aacgctctgt ccggttattt
     1141 caaccctgcg attggggcgc agacattcct tcagtacttc gccctccaca tccgggcaga
     1201 acaccactgg tgcatcatat ccacggcccg ttgcatgtat cctttcagcc ctgaatcccc
     1261 gggcaataaa cca
//
LOCUS       DAZFFI010000357         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFFI010000357 DAZFFI010000000
VERSION     DAZFFI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.360"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1272
                     /locus_tag="ACHJ12_005329"
     CDS             <1..>1272
                     /locus_tag="ACHJ12_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO4992938.1"
                     /translation="LRGELTFIEKAQGIHKARLIYEESLQRQVTIRELATLLTDEGLP
                     VSHTSISRMEHALKYLYPWIPDLMESGLGRPQVTALLALRQDAERVWGQFAVAADTDA
                     EFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPSLDYDRWLLELDPKERNRR
                     HHFGEPEPVVVPLSVRGSSTVQPGAPVSGIRSEVPECQPDTDTEAMPESTVSIFPGTE
                     REQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGASSAENALRPEIQPDMYGG
                     KPVFSGDVMEDGAAGAVLASVMTGDTAPGTTDALPASVTHFAVGRDDIEHLQNEAFRL
                     AWELAESAGCAEEIAMDRENDLSAGFGATVEKCSPVVAFLAGLAGDAPRTVLPVSLKD
                     LLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRL"
BASE COUNT          272 a          318 c          403 g          279 t
ORIGIN      
        1 ctacggggcg aactgacttt tattgagaag gcgcagggaa ttcacaaggc tcgacttatt
       61 tatgaggaat ctctgcagcg tcaggtaacg atacgggaac tggcgacctt attgactgat
      121 gaagggttgc ctgtcagcca tacctccatc agccggatgg agcatgccct gaaatacctg
      181 tatccctgga tccccgacct gatggagtca ggactggggc gtccacaggt tacagcactc
      241 ctcgcacttc gtcaggacgc agaacgtgta tgggggcagt ttgctgttgc agccgatacg
      301 gatgcggaat ttgaccgcgt gttcggggaa agctgccgga agtttaattc gccggaactg
      361 tggtcactgg agatgttccg cgatgagctt atcggagatt tgttgcaggc cctgccacat
      421 ccctccctgg actatgaccg gtggttgctg gaactggatc cgaaagagcg aaaccgtcgt
      481 catcattttg gtgagccgga accggtggtg gtgcctcttt cagtccgtgg cagcagtacg
      541 gtacaacccg gggcaccggt atccgggatc cggtctgaag taccggagtg tcaaccggat
      601 acggatacag aggcgatgcc ggaaagcaca gtctccatat tccccggtac tgagcgcgag
      661 caacatggta ttacccggcc actgaaagcc gtgtctgata ctgacacaga gcgcactgaa
      721 aaatgcagtc gaccttctca ggaagagagc gcaggcaggg gggacagtgg ggcgtcttct
      781 gctgaaaatg ccctgcgtcc tgagatacag ccggatatgt acggtggtaa gccagtcttc
      841 agtggtgatg ttatggaaga tggtgctgcc ggagcagtac tggcatcagt tatgacagga
      901 gatactgctc cgggaaccac tgatgctctt cccgcgtcgg tgacgcactt tgctgtgggg
      961 cgggatgata tcgaacatct gcagaatgaa gcgttccgtc tggcctggga actggctgaa
     1021 tcagccggtt gtgctgaaga gattgccatg gacagggaaa acgacctgtc tgccggtttc
     1081 ggggcaacag tggagaaatg ttctcctgta gtggcgtttc tggcgggtct ggccggtgat
     1141 gcaccgcgga cagtcctccc cgtgagtctg aaagacctgc ttacaggggg cagtgcaccg
     1201 gaagcgtggc cactgctgga tgacgaccat gccgtgaagc tgctgcgtct tctgagtgtg
     1261 ctgcgccgcc tg
//
LOCUS       DAZFFI010000358         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFFI010000358 DAZFFI010000000
VERSION     DAZFFI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.361"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1272
                     /locus_tag="ACHJ12_005330"
     CDS             <1..>1272
                     /locus_tag="ACHJ12_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO4992939.1"
                     /translation="VRGELTFIEKAQGVHKARQIHEEQLGRTVSLRELADLLTHEGLP
                     IHYSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALRQDAERVWGQFAVAADTDA
                     EFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHTSLDYDRWLLELDPKERNRR
                     HHFGEPEPVVVPPSVRGSSTVQPGAPVSGSRSEVPECQPDTDTEAMPESTVSIFPGTE
                     RKQHGITRPLKAVSDADTERTEKCSRPSQEESAGRGDSGASSAENALRPEIQPDMYGG
                     KPVFSGDVMEDGAAGAVLASVMTGNTAPGTTDALPASVTHFAVGRDDIEHLQNEAFRL
                     AWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFLAGLAGDAPRTVLPVSLKD
                     LLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRL"
BASE COUNT          278 a          318 c          403 g          273 t
ORIGIN      
        1 gttcgcggtg aactgacttt tattgagaag gcgcaggggg tccacaaggc aagacaaatc
       61 catgaggaac aactgggcag aacagtgtct cttcgtgaac tggctgattt gttaacccat
      121 gaagggctgc cgatacatta ctccacaatc agcagaatgg aagatgccct gaagtatctg
      181 tacccctgga tccccgacct gatggaatca ggactggggc gtccacaggt tacagcactc
      241 ctcgcacttc gtcaggacgc agaacgtgta tgggggcagt ttgctgttgc agccgatacg
      301 gatgcggaat ttgaccgcgt gttcggggaa agctgccgga agtttaattc gccggaactg
      361 tggtcactgg agatgttccg cgatgagctt atcggagatt tgttgcaggc cctgccacat
      421 acctccctgg actatgaccg gtggttgctg gaactggatc cgaaagagcg aaaccgtcgt
      481 catcattttg gtgagccgga accggtggtg gtgcctcctt cagtccgtgg cagcagtacg
      541 gtacaacccg gggcaccggt atccgggagc cggtctgaag taccggagtg tcaaccggat
      601 acggatacag aggcgatgcc ggaaagcaca gtctccatat tccccggtac tgagcgcaag
      661 caacatggta ttacccggcc actgaaagcc gtttctgatg ctgacacaga gcgcactgaa
      721 aaatgcagtc gaccttctca ggaagagagc gcaggcaggg gggacagtgg ggcgtcttct
      781 gctgaaaatg ccctgcgtcc tgagatacag ccggatatgt acggtggtaa gcctgtcttc
      841 agtggtgatg ttatggaaga tggtgctgcc ggagcagtac tggcatcagt tatgacagga
      901 aatactgccc cgggaaccac tgatgctctt cccgcgtcgg tgacgcactt tgctgtgggg
      961 cgggatgata tcgaacatct gcagaatgaa gcgttccgtc tggcctggga actggctgaa
     1021 tcagccggct gtgctgaaga gattgccatg gacagggaaa gcgacctgtc tgccggtttc
     1081 ggggcagcag tggagaaatg ttctcctgta gtggcgtttc tggcggggct ggccggtgat
     1141 gcaccgcgga cagtcctccc cgtgagtctg aaagacctgc ttacaggggg cagtgcaccg
     1201 gaagcgtggc cactgctgga tgacgaccat gccgtgaagc tgctgcgtct tctgagtgtg
     1261 ctgcgccgcc tg
//
LOCUS       DAZFFI010000359         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFFI010000359 DAZFFI010000000
VERSION     DAZFFI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.362"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            23..739
                     /locus_tag="ACHJ12_005331"
     CDS             23..739
                     /locus_tag="ACHJ12_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4992940.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVGAAAG"
     gene            754..1128
                     /locus_tag="ACHJ12_005332"
     CDS             754..1128
                     /locus_tag="ACHJ12_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4992941.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     REVLDGGDAGDEPLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            1152..>1263
                     /locus_tag="ACHJ12_005333"
     CDS             1152..>1263
                     /locus_tag="ACHJ12_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992942.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPLGD"
BASE COUNT          276 a          303 c          407 g          277 t
ORIGIN      
        1 ttttttatta tcaggaggca gaatgtctgc tttgtatgaa cgctcacagc tgacgcaggt
       61 gatgatttca tctgccccgg cgactgctga aactatggat aaggcggaat atctgcgcct
      121 ggactgcacc atcaaggaag tccagttcac cgccggtcag aaacaggata ttgatgtgac
      181 cacgctctgc tccacagagc aggagaacat caatggtctg ggggcgtcgt ccgagatttc
      241 catgtcgggt aatttctatc tgaatcaggc ccagaacgcc ctgcgtgatg cttatgacaa
      301 tgacgcgttg tatgcgttta aggtgcagtt tccgtccggt aagggcttta aattcctggc
      361 ggaagtgcgt cagcacacct ggtcatccgg taccaacggc gtggtggctg caacgttctc
      421 actgcgtctg aaaggcaaac cggtgtcctt tgtggtaccg ctggcgtttg tgaaaaatct
      481 ggataagaca cttaccgtga ataccggtgc gctgctgaca atgtcagtca gtgccaacgg
      541 gggaacgccg ccgtataaat acgcctggaa gaaggatggt cagccggttg acgggcagac
      601 gacagacacc ttcagtaagc caggtgcgca gtccgccgat gcggggaaat atacctgcgt
      661 ggtgactgat tcggcagaga aagcacagag tgtgacgtct gttgaatgca ccgtgacagt
      721 gggcgcagcc gccggataag gggatgggtc atcatgaaaa aggatctgaa aacgctggcg
      781 ctggccagac tgtcagggtt tcgtcataaa acggtgaagg tgccggaatg gggtaatgtc
      841 agcgtggtgc tgcgggagcc ttcggcagag gcctggtatc tgtggcggga agtgctggat
      901 ggcggtgatg cgggtgacga acccctgtcg gtggtggcga aaacccgccg taacctggaa
      961 gcggatgtga cgctgttctg cgatgtcctg tgtgatacgg acctgcaacg ggtgttcact
     1021 ccggacgacc gtgagcaggt gctggccgtc tatggtccgg tacatgcccg gttgctgcgt
     1081 caggcactgg aactgatcgc tgatgcagag tcggccagaa aaaagtagcc cgcccggaaa
     1141 ttcgttttct gatgcgactt gcgctccgtc tggggcgcac cttatccgaa ctgcggcaca
     1201 gcctgagtgc gagcgaggcg atgatgtgga tggagttcga cagggtatcc ccgctgggtg
     1261 atg
//
LOCUS       DAZFFI010000360         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFFI010000360 DAZFFI010000000
VERSION     DAZFFI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.363"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..884
                     /locus_tag="ACHJ12_005334"
     CDS             126..884
                     /locus_tag="ACHJ12_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4992943.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(937..1044)
                     /gene="yniD"
                     /locus_tag="ACHJ12_005335"
                     /pseudo
     CDS             complement(937..1044)
                     /gene="yniD"
                     /locus_tag="ACHJ12_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
BASE COUNT          319 a          287 c          293 g          362 t
ORIGIN      
        1 tacattgcta agttattcgc gcctttttat aattcgttac ctttccaggg acgctaatct
       61 ttgatcctta agaaagtttc cgggaccgcg atggtctctt tttttgttgt acggactctt
      121 gataaatgaa gcttttgaag acagttcctg ccatcgttat gttggcgggg ggcatgtttg
      181 cgtcactgaa tgcagctgcc gatgattccg tttttactgt catggacgat cctgccagcg
      241 ctaaaaaacc tttcgaaggt aatctgaacg caggatatct tgcacaatca ggcaatacga
      301 aaagctcctc acttacggct gacaccacca tgacctggta tggacaaacc actgcctggt
      361 cgctgtgggg gaacgccagt aatacttctt ctaatgatga gcgttcttca gaaaaatatg
      421 cggcgggcgg gcgtagccgt tttaatttaa ctgattatga ctatttattt ggtcaggcaa
      481 gctggctgac agaccgttat aacggctatc gagagcgcga tgtgttgacc gcgggttatg
      541 gtcgtcagtt tttgaatggc ccggtacaca gtttccgctt tgaattcggt ccaggtgtgc
      601 gttacgacaa atataccgac aatgccagtg aaactcagcc gctgggctat gcctccggtg
      661 cttatgcatg gcagttgact gacaacgcta aatttacgca gggtgtttcc gtctttggcg
      721 cggaagacac aacgctgaac tccgaaagcg cattaaatgt tgctattaat gaacattttg
      781 gactgaaagt ggcttacaac gtcacatgga actctgaacc tccagagtct gcaccggaac
      841 ataccgatcg tcggacaacc ttatcgctgg gctacagcat gtaaaattat ctgccggacc
      901 gtgttatgcg gtctggctgt cttcgtttgt cactgctcag ccccaaatca ttgctgccca
      961 acgtagcaac aacatagcgc cacagattgc cagtagcacc accaccatct ttcagtgttt
     1021 tttatcaaag cgtttagtcg gcataccagt gtcctgcata tctcttgtgc gtgataacgg
     1081 agtgtaacaa aagggacagg catcaaggga ttttttgtta ttacagccgt cgggtggcgt
     1141 tgtacacctg atcactgttt ctgcgtttta tgaaaaaaat aaaattatgt atcgatatac
     1201 aaatatcata aatagctggt tataaatacg tttttagttc atcagcacta aaactcaggc
     1261 t
//
LOCUS       DAZFFI010000361         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFFI010000361 DAZFFI010000000
VERSION     DAZFFI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.364"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            772..>1255
                     /locus_tag="ACHJ12_005336"
     CDS             772..>1255
                     /locus_tag="ACHJ12_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992944.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     N"
BASE COUNT          422 a          263 c          234 g          336 t
ORIGIN      
        1 tacacgcgta agcgaccaaa tttcaaatgg tcttatacag cgcgcacagt cgcaggaagt
       61 gctggcaaac aaatgaaaag aataaaagaa gtcaataccg ccattacgca gtaagaacat
      121 tccggcatca ccggatggat aaggatcaca caaccactgc tgtgtattgc ccgtaataaa
      181 gggcgcggtt aaaagaaacc ttcatcgtca tgactgacga tatctgctct caaaggtggg
      241 cagatttacc accaccagaa acatcctggc tgcacatccc ctttcaggtt acgggggtgt
      301 tgatcacccg aaggcgttcc tctcagatca acggaacatt ggtgcccacc gaagcgggat
      361 gtggtcaccc gaaggcgctc ctctcagacc acttatggag cattggtggt cgccgaagcg
      421 acagataatg tattctgagc atatcagagc gcataaaagg cgtcaattat ccatgctccc
      481 tcacgaaaca gaagctgaac tcttaacacc tgctatcgga actcactgaa taaacaacta
      541 aaaaggacat tgtgaaaatt aacatcaaca gtatgtaaag tgatttgttt tgtcttaaat
      601 gggtgaatgt aaaaaaatat tctatacaat ccacacatcc ttgtatcata tatcaagtaa
      661 acatccacag agataacagc ttatttcaaa taagcagatt ctgtttgata cttcagaaaa
      721 atagtatatg ctcctgattt actgatgtta taagttatac aggatagtta aatgtgtgat
      781 tttataaaat catgttatgc agcaccaaga attaacaaaa tggctgattt tttctcatca
      841 acagcatctg gagtaaaaca taaacctttg aagcatgtaa aaattgataa caggcttttc
      901 ccatctcctg agtatataat tttttctgac tatcttacga aaaacagtgg tccatttgtt
      961 aaccactact tttgcagtat tccatacagc aaagaagaag aatgcaggct gggtgtgact
     1021 attatgaaat atgcaagcca tcgaaataag ccattgagct tatggtgtct tggaatggca
     1081 gaaggagcca tggccagaac aatttctcaa ctatcaaaag gacaggtttt atcattaaca
     1141 accagcccaa caaaagaaaa tgaacctgta ttctttagtc atggttctca tattcactct
     1201 tactttattt gctccccatt ctttcttgtt catgaagaaa taaaaaataa caacc
//
LOCUS       DAZFFI010000362         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFFI010000362 DAZFFI010000000
VERSION     DAZFFI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.365"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..1142)
                     /locus_tag="ACHJ12_005337"
     CDS             complement(6..1142)
                     /locus_tag="ACHJ12_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4992945.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
BASE COUNT          375 a          298 c          219 g          361 t
ORIGIN      
        1 acctgttaaa actgatacat cataccaaca gcaagtatat tatcggttgc aactccagcc
       61 tttttggtaa attcgctttc atcaataagg ttaattttgt aatcaataag aagtgacatg
      121 tttttgttca gaaagtagtt tgcgccaaca tcaatatact caacaagaga ctgatcgcca
      181 tagtttacta ttccaccttt aacatccatc cctttggact gaacgtaagc cagtgaagga
      241 cgcaggccga aatcaaacat atactgagcc acaacttcaa tattccgtgc tttatctgcg
      301 atgcctgcgc taccaaatgg agtcatattt cgggtttcag agtagtcagc tgccagatac
      361 aggtcattat cattatactt gaccccaaca cctgcaaact ccgcatgttt tcccccggca
      421 atccagcgag gcgtaataac ttcttttcct tctgaatctt tcgatgtcat gaaacgttta
      481 cggccttctt tttcctgcag actggttctg tcggcattag tgtaagcggc aacgattcca
      541 aaaccttcat agtcatagct tacggagaaa ccatatccat caccattagc ttcaagtaaa
      601 ttatcacggt catttttacc ctgatattga gcagaaaaag taagtccatc aacaagcccg
      661 aagaaatctg tattgcgcca ggtcagaact ccggttgttc tgccggtcat atagttatca
      721 gagtaagtcc agctgtcacc accaaagtcc ggaagtacgt cagtcagagc ccccacatca
      781 taggcaacac catagtttcg cccataatca agtgaaccga gtatattgtg gcgaatacct
      841 gcaaatgcca gacgattttt atcgttgcgt acttcacttt ctgtcttaaa ccccaggaac
      901 tggtattccc actgaccaaa gccagtcagg ctctcattaa tttgtgtttc gcctttgaaa
      961 ccaagacgtg caaatgttct gtcgccatcc atagaaccat cagagatgta atgacgggct
     1021 ttaacgcgac cataaaaatc cagtttatta ccgtctttat tatagatttc tgctgcctgt
     1081 gttgcctcac acagcattaa tgcagaaacg acagagcaca caaccatact taattttcgc
     1141 ataaaaaaat cccctgtgca catttttagt atggttttaa aaattaaact atggcgaaaa
     1201 aattatcact atgcaaagat gtaacaaaat attgctcagt gatctatatt gtt
//
LOCUS       DAZFFI010000363         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFFI010000363 DAZFFI010000000
VERSION     DAZFFI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.366"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..669
                     /locus_tag="ACHJ12_005338"
     CDS             151..669
                     /locus_tag="ACHJ12_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4992946.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            879..>1252
                     /locus_tag="ACHJ12_005339"
     CDS             879..>1252
                     /locus_tag="ACHJ12_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO4992947.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATIL"
BASE COUNT          304 a          325 c          325 g          298 t
ORIGIN      
        1 ttttacgaca accgtaaatt ctagctcggt ttgtatgtaa aaaatactga aaaatgatgc
       61 ggttccgtga agtgagctgc atctgcctgt ctgggattaa cggcaggtca ttgtaagtag
      121 taattaacca cgttaacaag gagtttacaa atgccaacac cgtgttatat ctccatcact
      181 ggtcagaccc agggaaatat tactgcaggt gcatttaccg ctgattctgt cggcaacatc
      241 tatgtgcagg ggcacgaaga cgagatgctg gtgcaggaat tcctgcataa cgttactgtc
      301 ccgaccgatc ctcagtccgg ccagcctgcg ggtcagcgtg ctcacaagcc gttcattttt
      361 accgtggcgc tgaacaaagc tgttccgctg atgtacaacg cactggcctc cggcgaaatg
      421 ctgccaacca ctgaacttca ctggtggcgt acctctgtgg aaggcaaaca ggagcattat
      481 ttcaccaccc gcctgaccga ttcgacgatt gtcgatatga aactgcatat gccgcattgc
      541 caggatccgg cgaaacgcga gttcacccag ttgctcgaag tttcactggc ctaccgcaaa
      601 atagagtggg aacacgtcaa gtctggcacc tccggtgccg acgactggcg cgcaccgctg
      661 gaagcataag ttaagccaac agcatccggc tatcaggccg gatgctgttt ttgtattaag
      721 ccgtaaaaag aaaggttgtc agtaaggggt tacgttcacg ggagatggcg gatgccggaa
      781 acttcggcgg tagcgtgcct gaacgcaagc gtaacactgt actgtcacag ccgctttaac
      841 agcgaactgt ctgtttttcc ttaaccccgg aggttgctat gtcaaccgga ttacgtttca
      901 cgctggaagt ggacggcctg ccaccggatg cttttgcggt ggtctccttt catctgaacc
      961 agtcactctc ttcgcttttt tccctcgatc tctctctggt cagccagcag tttctctccc
     1021 ttgaatttca gcaaatactg gacaaaatgg cctacctgac gatatggcag ggcgatgacg
     1081 tacagcgccg ggtgaaaggt gtggtgacct ggtttgaact gggggagaac gacaaaaacc
     1141 agatgctgta cagcatgaag gtgtgcccac cgctgtggcg cacagggctg cgccagaact
     1201 tccgtatctt ccagaatgag gacatcgaaa gcatcctcgc gacgatcctg aa
//
LOCUS       DAZFFI010000364         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFFI010000364 DAZFFI010000000
VERSION     DAZFFI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.367"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..899)
                     /gene="tssI"
                     /locus_tag="ACHJ12_005340"
                     /pseudo
     CDS             complement(1..899)
                     /gene="tssI"
                     /locus_tag="ACHJ12_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
BASE COUNT          321 a          314 c          316 g          297 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgaacggctt gttccgcgcg ccacttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cacccttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 ggattccagg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca ggaaatcgta atcagtctca ccgtactgga cacaaaacta acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
      661 tcaaaccagg tcaccacgcc tttcacccgg cgctgtacgt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcgt ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctcca gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct
     1081 caacctcttg tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa
     1141 acctaaagtt aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt
     1201 aacattatta aaatgaatga ttgcataaga aatatatgct accgctcc
//
LOCUS       DAZFFI010000365         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFFI010000365 DAZFFI010000000
VERSION     DAZFFI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.368"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            134..700
                     /locus_tag="ACHJ12_005341"
     CDS             134..700
                     /locus_tag="ACHJ12_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFO4992948.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAIKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKANIPF
                     LIPMLNPKAFLFFAAFMPSFIKNNSNFNTDFLLLCIIFIIVSLLTDLIYICICLTIRG
                     RINSSFSKYVNFISSLFILLTGAYLVFL"
     gene            complement(849..>1248)
                     /locus_tag="ACHJ12_005342"
     CDS             complement(849..>1248)
                     /locus_tag="ACHJ12_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992949.1"
                     /translation="APRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
BASE COUNT          343 a          291 c          200 g          414 t
ORIGIN      
        1 ttctattcaa tagctgccag caattcgcaa aaaaacattt ctgactttat aatgttgctt
       61 cctgaggcag ccatacctat ggagtatgtt tattttaagg ttccaaccag aataacagga
      121 gttatataat cccatggact tctcaacatt aataacattc tctattataa ccttatcaca
      181 aacagtatcg attggtccgg gggtggcatt aatcatatct gaaagtctga catatggtgc
      241 aaaaaaagca ataacgacaa gtatatatat aagatttggc gaaattgttg tcatgctagt
      301 cacactattt tctatagcac tggcaatcaa acatcttgac acaataaaaa cttgtggtgg
      361 tggatatctg atttttcttg gtattactcg tctcatcaag agaaaaggat ctaaaggaaa
      421 caaagcaaat atcccttttt taataccgat gctcaatcca aaggcctttt tgttctttgc
      481 tgcatttatg ccatcattca taaaaaacaa cagtaacttt aatactgact ttttgttact
      541 ttgtatcatt tttataattg tgtcattact cactgactta atatatatat gtatatgttt
      601 aactataaga gggcgtataa attcatcatt ttcaaaatat gttaatttta tcagctcttt
      661 atttatactt ttaactggcg catatctggt ttttttatga tatgccgtca gggtgtgctt
      721 ccttacatat cacgccctct tccagacgtc gctggtcgtc gccctctgcg tcccttactt
      781 tcagcccctc tgagcataat cgcccctcca gaggagccca tcacaacgac aatgccagcg
      841 ggttaatttt atctgacccg gccttcagcc acatccttca cacgtttaac taatggctca
      901 ataaatttat ctttcagatg ccccggtgcc ggagatgcat acgttatata tatccgactt
      961 cctttgccat ttttttcaag ttcaagcgtc agatgatcat caaactcgcc accatcccag
     1021 tcactgccca ttttataata actaccgggc atggcatccc acatatgccc gttctgactt
     1081 attgctgctg cggcccactt cccgttcgca tgtccgttat tacgaatcgc atcgacttct
     1141 tcatttgaat aaaacttata gtaccgtttc agtcttcccg cagctgtatc cacatcaaca
     1201 gggacttcta tgacatgatc acgtttctct ctttttgccc ggggtgcc
//
LOCUS       DAZFFI010000366         1231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFFI010000366 DAZFFI010000000
VERSION     DAZFFI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.369"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..>1231
                     /locus_tag="ACHJ12_005343"
     CDS             24..>1231
                     /locus_tag="ACHJ12_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4992950.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGIFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVE"
BASE COUNT          281 a          271 c          398 g          281 t
ORIGIN      
        1 ggatttaaac tggcgaggtt ttaatggcaa ttattgatga tgtgataggc gtgttttccc
       61 ccggatggaa agcagccaga ctgcgttcaa gggcgttaat catggcctat gaggcggtga
      121 aaccgacccg gacacataaa gcccggcggg aaaatcgctc tgctgatcag ctcagtaaat
      181 acggtgcggt ttccctgcgg gagcaggccc gttttctgga tatcaatcat gacctggtga
      241 ttggtgtgtt tgacaagctg gaagagcggg tgattggtgc caggggaatt attgtggagc
      301 ctcagccatt acgaaaaaac ggggaaatgg cggctgagct ggctgcggat atccgccggt
      361 tgtgggctga atggtccgtg agtccggatg tgacagggca gtatacccgt cccgtgcttg
      421 aacgtttact gctgcggacc tggctgcggg atggtgaagt gtttgcgcag atggtcagtg
      481 gtgcgggaaa cggtctggaa cggacggcgg gagtgccatt ctggcttgag gcgatggagc
      541 cggattttgt tcccatgcgc acagatgaat ccgccggact gaatcagggg atttttcttg
      601 atgagtgggg aagaccgaaa aaatatctgg tttataaaaa ttatccggtc agcggccggc
      661 agagtgatac gaaagaaatc gctgccggaa aaatgatcca cctgaagttc actcgtcgtc
      721 tgcatcagac gcgaggctca tccatgttat cgggggtgct gatgcggatc agtgccctta
      781 aggagtatga ggatgcggaa ctgacagcgg cgcgtattgc tgcggcgctg ggactgtata
      841 tccgtaaagg tgacggacag gactatgaag atccggggat caaagaaacc gagcgggaag
      901 tccatatcac cccgggtatt atttatgacg atttgcgcaa gggcgaggat atcggcatgg
      961 tcaaatctga ccgtcccaat cccaatcttg aaactttccg caacggccag ttgcgtgcag
     1021 tggcagcagg cagtcgtctg agtttttcca gtgcggcgcg taactataac ggcacctaca
     1081 gcgcccagcg gcaggagctg gtcgagtcca cggatggtta cctgatcctg caggactgtt
     1141 ttattggcgc ggtaacccgc ccggtgtacc ggacatggct gaatatggtg gttgcggcag
     1201 gtctgctgaa aattccggcg gatgtggaga t
//
LOCUS       DAZFFI010000367         1224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFFI010000367 DAZFFI010000000
VERSION     DAZFFI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.370"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..66)
                     /locus_tag="ACHJ12_005344"
     CDS             complement(<1..66)
                     /locus_tag="ACHJ12_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992951.1"
                     /translation="MIYITIFMILPCPVPCSHVFLY"
     gene            147..1224
                     /locus_tag="ACHJ12_005345"
                     /pseudo
     CDS             147..1224
                     /locus_tag="ACHJ12_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          440 a          171 c          294 g          319 t
ORIGIN      
        1 atacagaaag acatgagagc aagggacggg gcagggcaag atcatgaaaa ttgtgatgta
       61 aatcacgatt ttcatcttgg ctttaacgcc tacaggtgat caaaatttaa cctttggaat
      121 aactaaaaag ataaaaaagg acccaggtga gtattcaaag tttgcttgat tatatttcag
      181 tgatccctga tatacgacaa caaggaaagg ttaaacataa attatctgct attttgtttc
      241 tcaccgtatg tgcagtaatt gcaggtgccg atgagtggca ggaaattgaa gattttggac
      301 atgaaagact tgaatggcta aagaaatatg gtgattttga taatggcatt ccggtcgatg
      361 acaccattgc acgcgttgtg agtaacattg acagtttggc ctttgaaaag atgtttattg
      421 aatggatgca ggagtgccat gaaatcactg atggtgaaat tatagcaata gatggaaaga
      481 ccataagagg ctcctttgat aagggaaaaa gaaaaggagc aatccatatg gtgagtgcat
      541 tctcgaacga aaatggtgtt gtactggggc aggtgaaaac ggaagccaaa agtaatgaga
      601 ttacagccat tccagagttg cttaacctac tgtatttaaa gaaaaatttg ataaccattg
      661 atgctatggg ctgtcagaaa gatatcgctt cgaagatcaa agataaaaaa gcagattatc
      721 ttctggcagt aaaaggcaat caggggaaat tacatcatgc attcgaggaa aaatttcctg
      781 taaatgtgtt ttctaattat aaaggcgatt cgtttagtac gcaggagata agtcatggaa
      841 gaaaagaaac acgtttgcat attgtcagta acgtaacgcc tgaacttttg tgattttgaa
      901 ttcgaatgga agggattaaa aaagctttgt gtagcattgt cattcaggca gaagaaagaa
      961 gataaatcag cagaaggtgt aagcatccga tattatattt catcaaagga tatggatgct
     1021 aaagaatttg cacatgctat cagagcgcac tggctgatcg agcacagtct tcattgggtg
     1081 ttagatgtaa aaatgaatga agatgccagc cggataagaa gaggaaacgc agcctaaata
     1141 atatctggaa taaagaagat ggcactgaat ttattaagag attgcaaaga cattaagggt
     1201 gaagaggaaa agaaagaagg ctgc
//
LOCUS       DAZFFI010000368         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFFI010000368 DAZFFI010000000
VERSION     DAZFFI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.371"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..177
                     /locus_tag="ACHJ12_005346"
     CDS             <1..177
                     /locus_tag="ACHJ12_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992952.1"
                     /translation="NDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTS
                     ADEILAYRVGWAQE"
BASE COUNT          346 a          236 c          307 g          330 t
ORIGIN      
        1 aatgatgtgg aggtgacagc agaagagctt atagcgctga gtgaagcggc agagcaggcg
       61 atgttcacca aagggatgga aatccacgtt cgccagcgca ccatgaagaa ggagctggaa
      121 aagctgacca gtgcggatga gatactggca tacagggttg gctgggcaca ggaataacag
      181 acaaaaagat gggggaccat cacttccccc atgaactgcg ttggctatgt aattattgtg
      241 aagagacctt acctatacag ataaagagtg ccacggaaaa gttaatcctg aaagtgatac
      301 cggtctcatt tccagagcat cctgttgata tggataattt ttcagatttc cggaagtgag
      361 agttttggtg tttccatacc agccacagtt gatgtgtttt atggcgccag gcagtgcgat
      421 taagggaaca gggataatga aaaaacgggg gtatcaccac ccccccctgg tacaaggaag
      481 gagtaactac aatgaaaaaa tcaatgaaga cattcagtgc tatgttatat ggcaaataaa
      541 gactgccaca gggaagtttg cagaaacagt gatgccgatc gtgtttctgt gctcttctct
      601 gataagagta atttttcaga tttccggaag tccgtatttt gcacgggccc ggttgtatgc
      661 ttcagactgc ggtggatatc aaggtcacgg agttggtcgg ttgcaaggaa tagattgaga
      721 attgagcgtg tttctggcac atgcctgact gtaatcatcc ctacaaacta cattgatgcc
      781 agaaacgcac tggtaacagg gcatcgtgta tttcagtatg tccagttttt gttgcggtga
      841 ttgcataaaa aatccccctt gaggggtgaa atatcaccgg aattttcggc aagtattttt
      901 atcgggttga gtgtatttct ggggctctgc tgaggcaatt cactgaatgt ggcttttgtg
      961 ctcgctggaa atgcactcat acacttttta acgcgatttt tttcaggcaa tagtttttaa
     1021 tttgttagcg ttctcgctgt caggcacgtt ttgagcacgt ttccgactct cagctacagc
     1081 aatccacgac tagggacagc acttacgttt tacttagcgc accgtaaaac tccgctcttc
     1141 agggtaaggg cttacatgaa gaaaaaaaat aaacttgtga atatgaaagt aaatactata
     1201 aaaagatggt tcctctttt
//
LOCUS       DAZFFI010000369         1208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFFI010000369 DAZFFI010000000
VERSION     DAZFFI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.372"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..542
                     /locus_tag="ACHJ12_005347"
     CDS             <1..542
                     /locus_tag="ACHJ12_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992953.1"
                     /translation="SSHGQELSGVSPCGERAFVILPWVGGPPGMLVGAAVGAVVIGGG
                     IVIYNNREAVGDAIEKSMEMRGEAELLNAQSQMEFWRDPYGAFSSVFGNDDSSKENPN
                     VGKDLTDEEKNCLGSGSTGGSGGWEPDEDGEIRNTYRSIKDAPQYPRGFRNVQNGTTR
                     NVVKDQQLLKQLRKVDSGK"
     gene            808..1002
                     /locus_tag="ACHJ12_005348"
     CDS             808..1002
                     /locus_tag="ACHJ12_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HFO4992954.1"
                     /translation="MNAASHSKNLIDIIDSGCELEDISSIIPENLSGNIDKLINQTLD
                     FIRGAPDFGGAVDKEIILVK"
BASE COUNT          367 a          169 c          332 g          340 t
ORIGIN      
        1 aaagcagcca tggacaggaa ctatctggcg tcagtccttg cggggagcgg gctttcgtaa
       61 tcttgccctg ggtaggaggc cctccaggga tgttagtcgg tgcggcagtt ggagcggtgg
      121 ttattggtgg tggtatagta atatacaaca atcgcgaggc tgttggggat gcgattgaga
      181 aaagtatgga gatgcggggt gaagccgaat tattaaatgc acaaagtcaa atggaattct
      241 ggcgagatcc ctatggtgct ttttccagcg tgtttggaaa tgatgattca tcaaaagaga
      301 atccaaatgt cgggaaggat ttgacggatg aagagaagaa ctgtttgggc agtggcagta
      361 caggaggttc gggagggtgg gagccagatg aggatggcga aataagaaat acctacaggt
      421 ctattaagga tgctcctcag tatcccagag ggtttaggaa tgtccaaaat ggcactacaa
      481 ggaatgttgt aaaggatcag cagttattga aacaattacg taaagtagat tcaggaaaat
      541 agagtaaagt ttataaagat ggatttgatg cgtcaggtcg aaaagtgtcc attcactatt
      601 ttgaaagtca gagtggtcga gtatttaatg taaaagtaaa accttattgg agtaatttat
      661 aatgagaatt gattatagta ttagcaatga acacgcagag aaatttatca gtcttttagt
      721 gttgggtgtt ttatattcta ttgaaaaaaa gcaatatcta tagatgaggc agaaggtttt
      781 atatttaagc catctacgtc aaaaacattg aatgctgcat cgcactctaa aaacttaatt
      841 gacattatag attctggttg tgagcttgaa gatatatcta gcatcattcc tgaaaattta
      901 tcaggtaata ttgataaatt aattaaccag acgctggatt ttattcgagg tgctcctgat
      961 tttggcggtg cagtcgataa ggaaattatt ttagtcaaat aaaaatatat cgttaatata
     1021 tgttggtgat gttttgttga gggctttttt attgttagtg gtataaatat aagggagtga
     1081 gaatgagcgg aaaaccggcg gcgcggcagg gcgacatgac ccgcaaggga ctggatattg
     1141 tgcagggttc agcaggggtg ctgataggcg cgccgacggg ggttgcctgc tcggtgtgtc
     1201 ctaaaaaa
//
LOCUS       DAZFFI010000370         1205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFFI010000370 DAZFFI010000000
VERSION     DAZFFI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.373"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            608..754
                     /locus_tag="ACHJ12_005349"
                     /pseudo
     CDS             608..754
                     /locus_tag="ACHJ12_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(804..1157)
                     /gene="cmi"
                     /locus_tag="ACHJ12_005350"
     CDS             complement(804..1157)
                     /gene="cmi"
                     /locus_tag="ACHJ12_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO4992955.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWPEDKGLACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
BASE COUNT          339 a          284 c          224 g          358 t
ORIGIN      
        1 ttctttttct atccattact ttctatccca ttaccataca aacactaacg ataatgatta
       61 tcgtttacat agtcaagagt gaagggtagg ggagccctca cccccctata aggggtccgc
      121 ctggaaaacg gagtttccca cgtcaagaga attgagttga acaagtggtt acatccatcc
      181 aagccagcta actcactaaa cgtaaaggat gtacgaaatg ttcctaccgt ccgataacca
      241 tttggcttac ttccgtacat tcccttgcgt agatgttcag aattgtacga gttcggacac
      301 caaaaccgct gttgtcccgc cattctggcg attcctgaaa gtcatttgac taaaaaatag
      361 gggctatttc gaattttctg gacactcctg acgcggagtc tgtgacttca acctgacacc
      421 ttacgctctt agcgtacgat tttttccgtt tcgtgagttg acccctttat tttccaaatt
      481 ccaaactgcc ccctacattt atacaatgtc cggcagggca tagaagggga gaaagaaaat
      541 acctgggcga agccgctgaa catcattctg gcgatttcaa taaaggcatt tgctcgcgct
      601 ctgtcattat gatgataaat acgattttaa cgccagcact caccgtggcg tcatcggaga
      661 gtcgctcaca aggctcgggg cgatgtgttc tggtaaagag taccagatac tgcttcctgg
      721 tgaaattcac attaaagaaa gtggtaagcg ataatataag ggccgggatt ccggcctttt
      781 tatgcagcct ttataacagt gtattagtca ttcgcatatt caacatatcc ctcctcacaa
      841 tggtacatgc cataatacat tctcttaccc tccggacctt cagctttaaa tgggacaaga
      901 agtgtttttt catcttcaac atctgttaaa cttctttcaa tcttttccca tgtaattttc
      961 gggatttctg taccaagaag ttgtacatcg ttgtcccagc gtttcattct ttgcaggtaa
     1021 tcatttttta caaacgttct ggcctgttca tcgctgacct gatagcatgc cagaccttta
     1081 tcttcaggcc agaaaaaaac agcagcaaga gcaataatcg ttaaaataaa aataaatttc
     1141 atgctaatta ctttcataaa atctccacaa cttctgtcgt tcattcgata taaaaaaaca
     1201 ttacg
//
LOCUS       DAZFFI010000371         1203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFFI010000371 DAZFFI010000000
VERSION     DAZFFI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.374"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..474
                     /locus_tag="ACHJ12_005351"
     CDS             <1..474
                     /locus_tag="ACHJ12_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4992956.1"
                     /translation="APAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGATRVDGKPVI
                     PGMKLSKEKCDQVNAIERDKALAWVAKNIRVPLTEPQKAGIASFCPYNIGPGKCFPST
                     FYRRINAGDRKGACEAIRWWIKDGGRDCRTRSNNCYGQVIRRDQESALACWGIDQ"
     gene            471..938
                     /locus_tag="ACHJ12_005352"
     CDS             471..938
                     /locus_tag="ACHJ12_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4992957.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCIN"
     gene            962..1186
                     /locus_tag="ACHJ12_005353"
     CDS             962..1186
                     /locus_tag="ACHJ12_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992958.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          299 a          280 c          342 g          282 t
ORIGIN      
        1 gcgcctgcgc ctgaaatcct cgaccagttt cttgacgaaa aagaaggtaa ccacaccacg
       61 gcataccgtg atggtgcggg tatctggacc atctgccgag gtgccacccg ggtggatggt
      121 aagcctgtga ttcctggcat gaagctgtcg aaggaaaaat gcgaccaggt taacgccatt
      181 gaacgtgata aggcgctggc atgggtggcg aaaaacatca gagtgccact gaccgaacct
      241 cagaaagcgg ggatcgcgtc attctgccct tacaacattg gtcccggtaa gtgtttcccg
      301 tcgacgtttt acagacggat taatgcaggt gatcgaaaag gtgcctgcga agcgattcgc
      361 tggtggatta aggacggtgg gcgtgattgc cgcacacgtt caaataactg ctacggacag
      421 gttattcgtc gtgaccagga aagcgcatta gcttgttggg ggatagatca gtgagaagag
      481 taaccgcgat tatttacgct ctggttatct gcatcatcgt ctgtctgtta tgggctgtta
      541 atcattaccg cgataacgcc atcacctaca aagcgcagcg cgataaaaaa gccagagagc
      601 tggagctggc aaacgcaacc attactgaca tgcaggtgcg ccagcgtgat gttgctgcgc
      661 ttgatgcaaa atactcgagg gaattagccg atgcgagagc tgaaaatgaa actctgcgtg
      721 ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgctcc ggtaccgtgc
      781 gtgaagccac cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacacag
      841 ttgaacggga ttatttcact ctcagagagc ggttgatgat gatgcagaag cagctggaag
      901 gggcgcagga atatatccgc actcagtgta ttaactagta tttttgttat ccggagaatg
      961 catgaagaaa ttactggtaa ccgtaaagcc ttttcaggga acaattccgt tccgtgtttt
     1021 gcagcctggt cgtgttcttg ttgaaggtac gttcagtggt aaatgtacgc aatcatactc
     1081 ccgggtcttt cgggtggatg cctcgaatga agagctaacc gtggagtgtg ctgtgaatgc
     1141 agctaaatgc cgcatggtat cggctgcatt acagccagtg tgttgagcga ccttattatc
     1201 cat
//
LOCUS       DAZFFI010000372         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFFI010000372 DAZFFI010000000
VERSION     DAZFFI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.375"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1096
                     /locus_tag="ACHJ12_005354"
     CDS             <1..1096
                     /locus_tag="ACHJ12_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4992959.1"
                     /translation="KKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHY
                     FSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFA
                     GLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNND
                     FFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRTD
                     TQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKAQ
                     NFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVDY
                     KINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          330 a          264 c          269 g          328 t
ORIGIN      
        1 gaaaaaatta acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc
       61 tcaggcagct gaaatttata ataaagacag taacaagctg gatctgtacg ggaaagttaa
      121 tgccaagcac tacttttcct ctaacgatgc agatgatggt gatactactt atgttcgtct
      181 gggcttcaaa ggcgaaaccc aaatcaacga tcagctgact ggtttcggtc agtgggaata
      241 tgaattcaaa ggcaaccgcg ctgaatctca aggttcctcc aaagacaaaa cccgtcttgc
      301 atttgcaggc ctgaaattcg gtgactacgg ctcaatcgat tacggccgta actacggtgt
      361 agcatacgac atcggtgcgt ggactgacgt tctgccagaa ttcggtggcg atacctggac
      421 ccaaacagat gtgttcatga ctggtcgcac cactggtgtt gcaacctatc gtaacaacga
      481 cttctttggt ctggttgatg gtctgaactt tgctgctcag taccaaggca aaaacgatcg
      541 tagcgatttc gataactaca ccgaaggtaa cggtgatggc ttcggtttct ctgctaccta
      601 tgaatacgaa gggttcggta tcggtgcaac ttatgcgaaa tctgatcgta ccgacactca
      661 agttaatgca gggaaagttc ttcctgaagt atttgcttcc ggtaaaaatg cagaagtttg
      721 ggccgcaggt ctgaaatatg acgctaacaa catttacctg gccactacct attctgaaac
      781 ccagaatatg actgtatttg ctgatcactt cgttgctaat aaagctcaaa acttcgaagc
      841 tgttgcacaa tatcagttcg atttcggtct gcgtccgtcc gttgcttacc tgcaatctaa
      901 aggtaaggat cttggagtat ggggcgatca ggacttagtc aaatatgttg atgtaggtgc
      961 aacctattac ttcaacaaaa atatgtctac tttcgttgat tacaaaatca acctgcttga
     1021 caaaaatgac ttcactaaag cactcggtgt aagcactgat gacatcgttg ctgtaggtct
     1081 ggtttaccag ttctaatctg attacgaaaa agatatgttg cgggaggctt tgcctcccca
     1141 acatataagt ggctccctca agccacttcc tttaggagca caaccttgct t
//
LOCUS       DAZFFI010000373         1184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFFI010000373 DAZFFI010000000
VERSION     DAZFFI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.376"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            308..769
                     /locus_tag="ACHJ12_005355"
                     /pseudo
     CDS             308..769
                     /locus_tag="ACHJ12_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          337 a          271 c          298 g          278 t
ORIGIN      
        1 acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg
       61 agaggggtcg gtttgggaaa cggaattatt gtacgttagg agcatttttt aatcgatgcg
      121 gataagtaac tgattgattc atcgtgatca gctatcgcct ttaagttatt gcttttctct
      181 tattgcattt aggttatctc atgtccctaa aggtctgcca aataccgtat agcgttggag
      241 tacaccctac cgcaatagcg caaccgtaca atattgtacg tttcccaaac gcagccctat
      301 cgaggcactg tacgctatcg agtctgagat ccgcggcagt ccggcagaag agcgactggc
      361 ggtcagaaaa gccagaacgg taccgctaat gcagtcgttg tatgagtggc tccaggggca
      421 gatgagcacg ctgtcgcgtc actcggatac ggcgaaagcg ttcacctatc tgctgaagca
      481 atgggacgcc ctgaacgaat actgccgcaa tggctgggtg gagatcgaca ataatctgtg
      541 tgaaaacgcc ctccgggtag ttgcactggg gcggcgtaac tacatgttct tcggctctga
      601 tggaggaggc gagagtgcgg cagtgatgta cagcctgatc ggtagctgca aacttaacgg
      661 aatcgagccg gaaacgtggc tacgccacgt gatcagtgtc atcaacacct ggccagccaa
      721 ccgcgtgaaa gagttgttgc cctggaacgt cactctatct gtaaactaat ttttacccca
      781 cgtccttcac ggtgcgctta cggataatta atgtcactct gtatggatga aaaaactgac
      841 agcagaagtc tccgctgaca aagacaggaa tgatgattaa tgtcaacttg gccagtgagc
      901 cagcccctta aaacgccaga cagtggatgt atattaaaat aagagatagg ctgggaaaat
      961 ataaagagta actgcgcttt ctgagtaaaa aaacaacagc agcaggtact tcaaaaccgt
     1021 gatgcactgt cagttgcaaa gacgaatgta cagttacctt tgttaccaga ggacaagaag
     1081 tgccccgggt attaccgtga aagaaatcag tgaacattta ttgaccataa ccacattgct
     1141 tctccgtaga ctgttgattt gaaagagtta acatccccgt aaaa
//
LOCUS       DAZFFI010000374         1182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFFI010000374 DAZFFI010000000
VERSION     DAZFFI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.377"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            336..>1182
                     /locus_tag="ACHJ12_005356"
     CDS             336..>1182
                     /locus_tag="ACHJ12_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992960.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIIIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DATALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTATLA
                     AARTHS"
BASE COUNT          361 a          300 c          268 g          253 t
ORIGIN      
        1 accagcgtac tcgtcttccc tgtgccggaa aacgcattca caaccagttg ctgcccctgc
       61 cagttaataa tggcgtactg ttcatctgtg ggattaaaag acatgcattc tcttctccgg
      121 aaaaacattt ccgtaatctg atgcatcagg ggtatgccca ccacaaataa ctctgtacag
      181 caacagaaaa atttccctgg tcatatgatg aattaatcat cgccatcaca ccggatgctt
      241 ctcatcctgt cctcagaaag acaggtggtc tcctgtctgc cgcgcttatc caggataccg
      301 ggacctgaaa ggtcataatc tggcaggaaa aaataatgtc cgaagtaaaa aaacacgttt
      361 cttattcatt aacagttgca gctatgctgt cagccgccat cgtctgtcag acagcacatg
      421 ctgatgtcaa cacaccgaac atcaccggag aaaaaaacaa tacaacaaca cgattctccg
      481 gaaatattta cggcagtgaa aatactgtca catcagagtc aaacagtctg gttgtgggta
      541 acagtaattc cgtggaggga gggtataaca acattattat tggtaattcc tccacggtca
      601 aagcttccgg tttgatcaga aaagtgggcg aagatattgt gctggatggt tacagcgtca
      661 gcctcggtaa tgccaccaga attaacggtg attcatctgt tgccataggt ctgacatcgt
      721 ccgtaaccgg gaacgactcc gtggcactgg gcagtcattc cagtgctaac ggaaacaata
      781 tcgtatccgt cggctctgac acactgaaac gccgcatcac aaatctggca caggggaaag
      841 atgaccatga tgcggtaaat ctgatgcaga tgaccagtgc tgttaaccgg gaagctggag
      901 aaagaataaa atcaacaaac aacctgcaga atcagcttaa taccctctct ggtcaggtca
      961 taactgaaac cagagcccga acagaaggtg atgcaacagc gctggctgca gccagagccc
     1021 acagcgatga aaacgacaaa agaacactgg tcagtgcgaa tacctatgct gaccagaaag
     1081 aaaccgccat caataaccgt actgacggat tactgacgac cgaacgccag gcacgcaccg
     1141 cgggcgatac tgcaactctg gctgctgcca gaacgcacag cg
//
LOCUS       DAZFFI010000375         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFFI010000375 DAZFFI010000000
VERSION     DAZFFI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.378"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1061
                     /locus_tag="ACHJ12_005357"
     CDS             <1..1061
                     /locus_tag="ACHJ12_005357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992961.1"
                     /translation="DAIDRIGTVIYTGNVNMTQGHGITADSGQKVKIADDDLQKDSKS
                     FLAIKAHEANVGTNVYVISKNGANNVALLEMTEGDNIITTELSLGNLGVSRWVRPMVV
                     DTGAGDDTIVTGRDLGIGYGDLWTEVNTGIGDDILIVGFGRGGYDVVYGKDGSYRIEE
                     TAKYQRLDGDIIIPDNDSGYPNNGGFIEKTTVTMGDGNDSLLLLGGNSLAALKSSTLD
                     LGAGNDVIDIVANIDNASNIKGGSGFDTLVIHQGNATSNTFSGFEKIELKDGAALNLL
                     AENLNSASIEGNKLKVTGDGSSRVDLGNNGDNLSDNGYNWIKGSTESEGDITYNIYTH
                     ASVPDVQVLIDERITNVV"
BASE COUNT          344 a          182 c          297 g          357 t
ORIGIN      
        1 cggatgcaat agatcggatt ggaaccgtta tctatacagg caatgtaaac atgacgcaag
       61 gtcatggaat tacagctgac tcaggtcaaa aggtcaaaat cgctgatgat gatttacaaa
      121 aagattctaa atcattcctg gccattaagg ctcatgaggc aaatgtaggg acaaatgttt
      181 atgtaataag taagaatggt gctaataacg tcgctctact ggaaatgaca gagggggata
      241 atataattac gactgaactg tcactgggga atctgggcgt ttctcgctgg gtaagaccaa
      301 tggttgtcga tactggtgca ggtgacgata ccatcgtgac tggtcgtgat ttggggattg
      361 gatatggtga tctatggacc gaggtaaata caggtatcgg tgatgatatc ctgatagttg
      421 gttttggtcg aggcggttat gatgtcgtat atgggaagga tggttcttac cgcattgaag
      481 aaaccgctaa gtatcaacga ctggatggag atatcataat acctgataat gattcgggat
      541 acccaaataa tggtggtttt atagagaaaa cgactgttac tatgggagat ggaaatgaca
      601 gcctgttatt gttaggaggt aattctcttg ctgcgctcaa atcatcaacc ttggatttag
      661 gtgctggtaa tgatgtaatc gatattgtgg ctaatattga taatgcttca aatattaaag
      721 gtggttctgg atttgatacc ttggttattc atcagggtaa tgccacaagc aacacattct
      781 ctggttttga gaagattgaa ctgaaagatg gagctgcact gaatttgttg gctgagaatt
      841 taaattctgc ctctatagag ggtaataaac tcaaagtcac aggtgatggt agttctcggg
      901 ttgacctcgg taataacggg gataacctca gtgacaatgg gtataactgg attaaaggaa
      961 gcactgagtc tgaaggggat atcacttata acatttatac acatgcttcg gttcctgatg
     1021 tacaggttct gattgatgag agaatcacta atgtagtgta acaatagtat cccctcacag
     1081 tcgttgctgt ggggggatct ttttcgttgt aagttctact ccaggtatgc tcttttactg
     1141 aaaaaattta tctgagaata atgctttttg tttcttcttg
//
LOCUS       DAZFFI010000376         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFFI010000376 DAZFFI010000000
VERSION     DAZFFI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.379"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..111
                     /locus_tag="ACHJ12_005358"
     CDS             <1..111
                     /locus_tag="ACHJ12_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4992962.1"
                     /translation="MPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            613..1071
                     /gene="tnpA"
                     /locus_tag="ACHJ12_005359"
     CDS             613..1071
                     /gene="tnpA"
                     /locus_tag="ACHJ12_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFO4992963.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT          356 a          222 c          321 g          278 t
ORIGIN      
        1 atgccggtgc cttatggtga aatgctggtt ggttcacgac ggatatccca ggacatcagc
       61 acccgtgatg agggcggaga cgggaaagtg gtggttatcg gacggggatg aaaataaaaa
      121 aatcccgcag agttagcgga gctgcgggaa ataacgatga agattaacaa gaatgaattg
      181 tttttctcgg acagggcaat gtaacgcccc ggaatgatat ctgccacgga gacgtacaga
      241 aaatgtgaaa aaattcagat ttttttgccg ggggtaaaaa aatcccgcag cgttcacagt
      301 tctgcgggaa ataacaataa agactaactt taaggagttt tgttttattg accataaaaa
      361 ctgtaacgct gcgtcattat agcggctaca ggcaattgcc ggaaatgtga agaatttcag
      421 aaattttatt ccgtcatgac acaggcaccc tccggggtgc ctgttgtttg tgcacagaaa
      481 acccccagct aggctggggg ttccggaaag ctttcagctt tgagccagtt attaaaaccc
      541 cttttgattt gttaaaacac cttgcggtct ggcaactgca agtgtcaaac aagaaatcaa
      601 aagggggtcc caatggggaa cgaaaagagc ttagcgcaca cccgatggaa ctgtaaatat
      661 cacatagttt ttgcgccaaa ataccgaaga caagtgttct acagagagaa gcgtagagca
      721 ataggcagta ttttgagaaa gctgtgtgag tggaaaagcg tacggattct tgaagctgaa
      781 tgctgtgcag atcatatcca tatgcttgtg gagatcccgc ccaaaatgag tgtatccggc
      841 tttatgggat atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt tggtgatttg
      901 aaattcaaat acaggaacag agagttctgg tgcagagggt actacgtcga tacggtgggt
      961 aagaacacgg cgaagataca ggattacata aagcaccagc ttgaagagga taaaatggga
     1021 gagcagttat cgattcccta tccgggcagc ccgtttacgg gccgtaagta acgaagttgg
     1081 atgcaaatgt cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag ccttataggc
     1141 gcatttgaaa aacctccggc tatgccggag gatattt
//
LOCUS       DAZFFI010000377         1162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFFI010000377 DAZFFI010000000
VERSION     DAZFFI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.380"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..492
                     /locus_tag="ACHJ12_005360"
     CDS             211..492
                     /locus_tag="ACHJ12_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4992964.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            498..986
                     /locus_tag="ACHJ12_005361"
     CDS             498..986
                     /locus_tag="ACHJ12_005361"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4992965.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
BASE COUNT          318 a          276 c          264 g          304 t
ORIGIN      
        1 ccggtgtact ccggatgtcg ctgctggtaa ataatctgag cttttatctg catgcaatgg
       61 tcaaagatcg tggcgttaac atcccccgga tagttttgtc tgaatctgct ttttcttttc
      121 aggcagaatg ccagtctgcc gtcccggtgt cagactgtaa tttgcacaat acggcaattt
      181 gccgtacagt gtattcagga ggtacaaatc atgccatctg caacacaaac tgcccgactg
      241 gaagcaagga tcagcacaga actacatgcc atgctgaagc gcgctgcaga aatccagggg
      301 cgcactatga ccgattttat catttctgcc gttcaggatg cggcccaacg cgctatcgaa
      361 caaaccgata taatccgact gtcccggaca gatcaggaaa tttttgctca ggcattactg
      421 tcaccaacag agccgaatga tgcgcttaaa cgggccttta aacgccgtaa taatatgctg
      481 tacacaaaat aaatataatg tttcaactga caccatttaa cagcacatat gacagaacct
      541 catttttatg tggctctgat gctctggacc gttacataaa agagcatgtc acacaggata
      601 ttcgacgacg catcgcggcc tgttttgtcg ctgtagataa cgatcatcat atcatcggat
      661 actatacgtt atcagcggcc agtatattat taactgactt acctgagcat ttgagaaaaa
      721 agcttccgcg atatcccact attccggcta ttcgtatggg acgactggca gtaagtctgt
      781 catcaaaagg aacggggctt gggggagcac ttttagctga tgcgctatat cgtgcataca
      841 catctgaaat tgcagcatat gcgcttattg ttgatgcaaa agatgacacg gcagtcgcat
      901 tttacaaaca ttatggcttt atggcgttcc cggagtccgg aagaacttta ttcatgccat
      961 tggcatcatt atccacaata aaataaacag aataaatcag ggaaatacgg tgcttgtggc
     1021 aacgtcagcc cacgaagtta aatgttaacg ggcaacacgg cggcaggaaa tctgccgccg
     1081 gattgttgtg aaggctgacc acactgcgcg ccctttttcc ttgattatcc cgctcagtcc
     1141 agtggtctgt tccggatggg gc
//
LOCUS       DAZFFI010000378         1161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFFI010000378 DAZFFI010000000
VERSION     DAZFFI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.381"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..552
                     /locus_tag="ACHJ12_005362"
     CDS             13..552
                     /locus_tag="ACHJ12_005362"
                     /inference="COORDINATES: protein motif:HMM:TIGR03761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO4992966.1"
                     /translation="MPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHL
                     AWLDGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFH
                     YGLISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPD
                     PDVMSGKKRSVYSPPVKAG"
     gene            555..>1161
                     /locus_tag="ACHJ12_005363"
     CDS             555..>1161
                     /locus_tag="ACHJ12_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO4992967.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLTRLYTLKARERGISGVLSVGRVQTPTLAMVV"
BASE COUNT          284 a          243 c          351 g          283 t
ORIGIN      
        1 gacatcatca gtatgcccag ggctattgcg tttgcgggtc aggcaaccag agattcagcc
       61 agggataatc cttatgcaga tatgatgtta gtcaggctgg aaaatgccct gacgaaggca
      121 actgaaacaa ttgaaaagca tcttgcgtgg cttgacggga tccttaaaaa cctgccggga
      181 cagatctccc tttctgatat taaatcctct tcgccggtaa atattggcgt gtacagtagt
      241 tctccggtcg gttatcgatg tgtatggctt ctgatcggat atgatcgact ggtcatgaag
      301 gtatttcagg tatttcatta cggacttatt tcccgcacaa aacgggatga gttgctgaat
      361 cagggagggt atgcggtacg caggatttac gggattattc aaaattataa atcagtcagt
      421 gttacacgtg ctgatatttt gtccggcaca gcccgtggaa aggaggcggt taaccgcatg
      481 ggggagccgg atcctgacgt gatgagcggt aagaaacgtt ctgtttattc accaccggtt
      541 aaagcggggt gagtatgcaa ctctttctgt gtgaaaaacc ttctcaggcg aaagatatcg
      601 cccgtgaact ggcgctcagt aagcggggcg acggatacat cggagatgac aatattgctg
      661 tcacctgggc gataggacac ctgctggaac tggctgagcc ggaagcttac ggtgaacagt
      721 tcggttcccc atggcgttct gatgtgttgc cgctgatacc ggaacaatgg cagatggtgg
      781 tgaagccgaa ggtgaaaaaa caacttattg tgatccgggg gctgttaaaa cgcgcttctg
      841 aagtggttat tgccacagat gccgatcgtg aaggagaagt gatagccaga gagttgctgg
      901 agtactgtca gtacagaggg cgtgtcaggc gtctgtggtt atctgcactg gatgaggcca
      961 gtatcaggaa ggcgctgaat aatattctcc cgggcgaaca gactgcgctt ctcagtgaag
     1021 ccggtaaggg gcgtgcccgg gctgactggc tgacagggat gaatctgacc cggttgtata
     1081 ccctgaaggc acgggaacgt ggaatttctg gggttttgtc agtgggccgg gtgcagacac
     1141 ccacgctggc aatggtggtc a
//
LOCUS       DAZFFI010000379         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFFI010000379 DAZFFI010000000
VERSION     DAZFFI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.382"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            185..364
                     /locus_tag="ACHJ12_005364"
     CDS             185..364
                     /locus_tag="ACHJ12_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4992968.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            405..650
                     /locus_tag="ACHJ12_005365"
     CDS             405..650
                     /locus_tag="ACHJ12_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4992969.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGEPTPDSDMMM"
     gene            727..942
                     /locus_tag="ACHJ12_005366"
     CDS             727..942
                     /locus_tag="ACHJ12_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4992970.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            946..>1148
                     /locus_tag="ACHJ12_005367"
     CDS             946..>1148
                     /locus_tag="ACHJ12_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992971.1"
                     /translation="MNYEEMNQRISRLENEITELNKKLSVLMPSEEEKKRLDEQFAAF
                     YDYCRKVMSRNLAECFRIQKNRER"
BASE COUNT          309 a          248 c          332 g          259 t
ORIGIN      
        1 attgacagtg tgaaggtgga agtgaacaac gcggacgccg cggcatgata aaaaaagccg
       61 ccagttgcct gagacctctg gcggtgtgag attcatggag aatcaaggaa agatactacc
      121 actttcgtca tcggcatttt taaatgaaaa ctgtttactt agtcaaccat aacggtaaga
      181 aattatgaca tttattcatc aggtgatgct gtacttctgt acggcggtct gtgtgctgta
      241 tcttctttcg ggtgggtaca gggccgtgcg cgatttctgg cgcaggcaga ttgataaaag
      301 ggccgcagag aaaatcagcg ccagtcagtc agccggaagc aaacccgaag agccgctcat
      361 ttagctgcaa ctttcttaat cacacctttc gacgagaaaa tcccatgtca gaaattacat
      421 ccctggtcac tgctgaggca gtgaaggaag tcctgcgctc tgaagaagtc ctgagcgcac
      481 tgaaacagaa actccgccag aaccttgagt cgcgtcttga tgcagaagtt gatgccattc
      541 tggatgaact gctgggcgca ccggctgttc cggagccgga aggcattgcg ggtgagggga
      601 gtgcttcaga tggcggtgaa cccacacctg acagcgacat gatgatgtaa gcatgcgcaa
      661 gggaccatcg gtgtgtgccg gtggtcttta tattgttgtg agcttccggc ttgcgggaga
      721 cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg
      781 ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca gtgggcggca
      841 ataggcgtgc tggggagtct gctgtttggt ctgctgacat acctgacgaa cctgtatttc
      901 aaaatcagag aagaccggcg caaggcggca cggggagagt aggtgatgaa ctatgaagaa
      961 atgaatcagc gcataagtcg cctggaaaat gaaatcactg aactgaataa aaaactgtcg
     1021 gtgctgatgc cttctgaaga ggaaaaaaaa cgcctcgatg agcagttcgc ggcgttttac
     1081 gattattgcc ggaaagtgat gagcagaaat ctcgcagagt gtttccgtat tcaaaaaaac
     1141 agagagcg
//
LOCUS       DAZFFI010000380         1141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFFI010000380 DAZFFI010000000
VERSION     DAZFFI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.383"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..120)
                     /gene="ydcD"
                     /locus_tag="ACHJ12_005368"
     CDS             complement(<1..120)
                     /gene="ydcD"
                     /locus_tag="ACHJ12_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO4992972.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKT"
     gene            complement(104..>1141)
                     /locus_tag="ACHJ12_005369"
     CDS             complement(104..>1141)
                     /locus_tag="ACHJ12_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992973.1"
                     /translation="QPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFP
                     AELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDH
                     RGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQLPGQQYDKESGLYYNRHRYYD
                     PLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLI
                     AANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEA
                     AGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIK
                     AKQGSSDASNY"
BASE COUNT          270 a          330 c          226 g          315 t
ORIGIN      
        1 tgttttatgt ccataaaata cgatgtcaga atttgaattg ttatcaaacc caaggtaaac
       61 aacaaataaa caaagggtaa ttaaaaaaat gacaagtttg cctctaataa tttgaagcat
      121 ctgaactccc ttgctttgct tttattggat tatctgcttt tataccatct aacccgaaga
      181 gttgcccagt gttatatttt tcaatacatg catcgctaca tttggcagaa aaatcgccac
      241 aagcaatacc gacagtgtta tttttaagat ccatgattct ttctttagct ggctgacctt
      301 gtctatcccc tgccgcctca tgatttttac ctatcacgtc agctattgtt gatccaaatg
      361 tcttagtcat tcgacacatc aagtaacaat gtctcattgc atcggcggcc ccattatgag
      421 ctccgccaac aatagcatcc atatcataac cgaactggta agcactgtta gtaatgctgt
      481 ttgcagcaat caatccaggt acaccagata cggcataaat tagtccacct gatgtcatat
      541 tctcaatcgc atctaacccc attgggtcta tcacttgtat gggattcaac ggatactgat
      601 acatattcca tccacctctc aacccaatcg ggtccggggt gatataccgc ccctgcaacg
      661 gatcgtagta ccggtgacgg ttatagtaca gccccgactc cttatcatac tgctgccccg
      721 gcagctggtg caggtgatgc gggttctcct cattaagctg gttgccccat tcatcatatt
      781 ccgcgctcca cgccgtattg ccgtcttcac tgataagcgc cagcggcagg ccccggtggt
      841 cacagtgata aagatgtatt tttcgctcag gcaaataaac cggctctatc tgtgccgcca
      901 gccgctccac cgtcagcccg cactgcgcaa gccactggcg gctttcactg ctcacgcagt
      961 ttgcccggat ttccccctcc agcctgtcca gcagccccac cagttcagcc ggaaatacca
     1021 cgccgtgccc gtcctcgctc ccttcctgct gtatcttctc cgccaggctg cggcattgcg
     1081 ctttctcccg ttcaccgttg tctgtttcga tgcggatgag cggcgcgaag ctccccggct
     1141 g
//
LOCUS       DAZFFI010000381         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFFI010000381 DAZFFI010000000
VERSION     DAZFFI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.384"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..966
                     /locus_tag="ACHJ12_005370"
     CDS             <1..966
                     /locus_tag="ACHJ12_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO4992974.1"
                     /translation="DVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALREKQSIAVL
                     EQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLKTTLGKLST
                     WTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKAALKAQELA
                     RYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLDSVARMIDE
                     TGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNAENELYSVG
                     LSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            971..>1140
                     /locus_tag="ACHJ12_005371"
     CDS             971..>1140
                     /locus_tag="ACHJ12_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFO4992975.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRR"
BASE COUNT          304 a          251 c          316 g          269 t
ORIGIN      
        1 gatgtcagcc ggagtgagtc ggaagccgag tattaccgtt ataaagttga tgaggctggc
       61 gaggaaacaa ttcgctcctt tgtttcatta tatcttgatg cgctaaggga aaagcaatcc
      121 attgcggtac ttgaacagag tctctcccgg cataacgcaa ttcttaatga tcttaacacc
      181 atcagtattc acgataccgg gcgggaatct gaatttgttc aggccgaagc ccgcaggctg
      241 atggtccggc agcagataaa tacccgcacc cgagtgctca aaaccacgct gggaaaactt
      301 tcgacctgga ccacaaatcc ggtaaccgaa gccgatcttg aaaatccttt ttcccggatg
      361 acagagtcca gattgttaac tgattttacg cagacgcctc agaaagggaa tccgtcgtgg
      421 cttgccagtc aggctgatgt agagagtaaa aaagcggcgc tgaaagcgca ggagcttgcc
      481 cggtatcccc gggtggattt aacgggatca gtgacccggg atgacaggca gataggtgtc
      541 agtctgtcct gggacctgtt caaccgtaat gccagttatg gtgtcagtga aaaggccgcg
      601 cagatagctg cagctaccgg acgactggac tctgttgccc gaatgattga tgaaaccgga
      661 cgattatcgc tgataacggt cagacaaagt cggatggaaa tggaaacgct cagacgtcag
      721 gagcaggcct ctgccagagt tgtggacttt tatcgtcttc agttccgggt ggcaagaaaa
      781 acactgattg aattactgaa tgcagaaaac gaactgtaca gcgtcgggct ttcccgggtt
      841 cagacagagg atcagatgct ccacggaatg ctggattatc tgtattccca gggaatgctc
      901 ctgaaatgga gcggagtgaa tctttctggt gcagaagaaa aagtaacagg gcagaaggac
      961 agataaataa gtgaagagta tttttgaagg attatcacag gtaacggcat tactgggtac
     1021 acctgtatct gctgagacgc tggctgccgg tacagtccgg acagatgtct ccgggattga
     1081 ttttcgttct gttggcgagt ttcttcgcag tgaagggttt gataatcatc tttcccggcg
//
LOCUS       DAZFFI010000382         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFFI010000382 DAZFFI010000000
VERSION     DAZFFI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.385"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..860
                     /locus_tag="ACHJ12_005372"
     CDS             <1..860
                     /locus_tag="ACHJ12_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992976.1"
                     /translation="ALLSGGAATFDAVTTAMASAAPALGAMAVVAGPMGYDLLANDGR
                     AGKALSDGQLINSQAEMCYWTSSCPLDSPHENDNSSGSQPNVGKDLTDEEKNCLAGGS
                     TGSPGGWGPEDEENARNQERGFDIEELPDAHGKMHDTAVKGDAHIPKDKVERFMRGKA
                     SGNLDELQQEYDELINLRRDNQRAFAKEYPDGKKTIRALEDKMHNIDRSREMGQILED
                     AGIPDTPANNDMIIEKLLDSAQGDLPADRTTSVVIDGKNGSVRVNGVWVDMPDGAKRL
                     STIIVKVFK"
     gene            857..>1130
                     /locus_tag="ACHJ12_005373"
     CDS             857..>1130
                     /locus_tag="ACHJ12_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992977.1"
                     /translation="MNKNMVVDKLVNGLNSLLENGYIDPNSVDDEADALEYLGELLVQ
                     DETCCLSFCKKILYTLTSRDGVIKGAALDFLLLSDDWQDAFDYLMNN"
BASE COUNT          335 a          173 c          322 g          300 t
ORIGIN      
        1 gggcgttact ttcgggtggg gcggcgactt ttgatgcagt cacgacggct atggcgtctg
       61 ctgcccctgc tcttggagca atggctgtgg tcgctgggcc aatgggatat gaccttctcg
      121 ctaatgatgg gcgtgccggg aaggctctga gcgatgggca gttaataaat tctcaggctg
      181 aaatgtgtta ctggaccagt agttgtcctt tagatagccc acatgagaat gataattcgt
      241 cagggagtca gccgaatgtt gggaaggatt tgacggatga agagaagaac tgcctggccg
      301 gcggcagtac gggaagcccg ggaggatggg gaccagaaga tgaggaaaat gcccgcaacc
      361 aagagcgtgg ttttgatatt gaggaattgc cggatgcaca tggtaaaatg catgataccg
      421 cagtgaaggg tgatgcccat attcctaagg ataaagtgga gcgctttatg cgtggtaagg
      481 catctggtaa tttggatgaa ctgcaacaag aatatgatga gttgattaat ttaaggcgag
      541 ataaccaacg ggcttttgct aaagaatatc cggacgggaa aaaaacaata agagctttag
      601 aagataagat gcataatatt gatagatcaa gggaaatggg acaaatttta gaggatgctg
      661 gtatcccgga tactcctgca aacaatgata tgataattga gaagttactg gattcggcac
      721 agggtgactt acccgcagac agaacaacat cggtggttat tgacggcaaa aatggaagtg
      781 ttagagttaa cggggtgtgg gtagacatgc ctgatggtgc taagaggttg tcaacaataa
      841 ttgtaaaggt attcaaatga acaaaaatat ggttgtagat aaattagtta atggattaaa
      901 tagcttgctg gaaaatggtt atatagaccc taactcggta gacgacgaag ctgatgcatt
      961 agagtatttg ggagagctat tagtacagga tgaaacttgt tgtctgtctt tctgtaagaa
     1021 aattttgtat acattgacat ctagggatgg cgtaattaaa ggcgcggctt tagatttttt
     1081 acttttatct gatgattggc aggatgcttt tgattattta atgaataact
//
LOCUS       DAZFFI010000383         1111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFFI010000383 DAZFFI010000000
VERSION     DAZFFI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.386"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            245..562
                     /locus_tag="ACHJ12_005374"
                     /pseudo
     CDS             245..562
                     /locus_tag="ACHJ12_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          311 a          272 c          282 g          246 t
ORIGIN      
        1 ggggctattc ccgttgattt caatcgttca gtgatgctaa agttggtatt tggaaaagac
       61 aaaactattg agggtataga atataaacaa taataaaaaa tctgctaatg tataacagac
      121 agggcacaga agcagataac gggatactat ataatacctg tcagtccata gaaggaagtt
      181 atacgaatac cggagtgcta atgaacagaa tatttctgtg aagatgactt ccccgtaacg
      241 acaccacaaa gccattgtct atgcgctgtt tcatcgcgtg gaactgagcc gcttcctgga
      301 agatggtgcg gtgccgctgg ataataatgt gtgtgaacgg gccatcaaaa acgtggtcct
      361 gggcagaaaa tcgtgtctgt tcgccggttc gcagatggcg ggagaacgcg ccgcgcaaat
      421 aatgagcttg ctggaaaccg cgaaacgcaa cggtctggag ccgcatgcct ggctgacaga
      481 cgtcctgatg cgtctgccgg agtggccgga ggagcgactg gcagagttgc tgcctcttga
      541 gggatttacc ttctccgggt gagtgatacc tgccgtcagg tgttcgtgca ccgggccata
      601 acctgcagtc gggagttgaa cgcctgacga caggaaatga gccagaccag caacctcacg
      661 ggctgacagc gaataagaac ctgaatcagc ccggagtcat tcccgggtca gtacatcatc
      721 acctgccatt aatccccgag tcagtacttc gcggcctgac gcgatcaccc tgtgcaacaa
      781 aataaatcaa ccgttatcag accacgatcg ttcgtatgcg ccgccgatga cgcgcgcctg
      841 aaaaacgcgt caagatgaga tgcccggaag cactctgctg actgggatca aacgccagaa
      901 gagcagaggt aagcaaaaaa gtccgccaac taaggacggg attaaattta tgctttaggg
      961 actgatggcg gagattttct ggctttacgt tcatctatcc atgcaaagat ccatgatctt
     1021 aacccagccc ccccccctgc gatcccactc aatgttatgg ctgctttaaa ttgtttttct
     1081 gaaaaataga aacaacctcc ttcccaaaaa t
//
LOCUS       DAZFFI010000384         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFFI010000384 DAZFFI010000000
VERSION     DAZFFI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.387"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..1055
                     /locus_tag="ACHJ12_005375"
     CDS             24..1055
                     /locus_tag="ACHJ12_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4992978.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKETEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIVLGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
BASE COUNT          320 a          243 c          263 g          272 t
ORIGIN      
        1 ttgtaattat ccatggagtc gaaatggaaa aaacacgtaa gcgccgtggt accggacggg
       61 tgacgctgca ggaggtcgct aactttgcag gtgtcggaac catgaccgtt tcccgcgcgc
      121 tgagaacacc ggaacaggtt tctgacaagc tgagggaaaa gatagaacag gccgtagaag
      181 agttgggtta tattcccaac agaactgctg gcgcccttgc atctggtcac agtcatacag
      241 tcgccgtact ggttccttcc ctgacagata aagccagctc ccgctttatg cagtcgttgc
      301 agcaggtgct taataaaaat gaattccagc tactgcttgg gtgtcatgag tataatcagc
      361 tcaaagaaac tgaaattctg atgacgcttc ttcaaggcaa cccggcggcg ttagttattt
      421 ttggttctca gttggctgat aaaactcacc agatactcga gaaaacaaat atacctacca
      481 ttaacgttgt cggttcccct ttcagtccgg ctcaaattac catcgaaaca gcattttttg
      541 aagcatcaca taaactgacc gaacatctgc tggagcaggg ttataagaat atcggattta
      601 tcggtgcgca tatggacaac cgcctgcaac gtcagcaact gaacggctgg cataaagcaa
      661 tgctgagcca ttaccagaac actgatcttg ttatcaccat gccggatgcc gcaagtctgc
      721 aattggggcg ttacgcatta aacgagatat tactgcgaca accagagctg gatgctgtga
      781 tttgcagcca cgaagagatt gtgctgggta tcatgtttga gtgccagcgg cgtttgttaa
      841 aaatccctgg aaatatcgct gtagcttgtc ttgacggttc agacaattgc gatcaaacac
      901 acccaacttt gacatccatc cgtattgact ataaaaaaat ggggacagag acgggtaagc
      961 tgctgattgg actattgaat aataatcatg atgaatcaga agagtcgcgt atcgtgcaat
     1021 tcaactacca gatagaatta cgtcagagta catgagccta aaagagcact attacaaagt
     1081 tagctattgt ggtaggga
//
LOCUS       DAZFFI010000385         1092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFFI010000385 DAZFFI010000000
VERSION     DAZFFI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.388"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1092
                     /locus_tag="ACHJ12_005376"
     CDS             <1..>1092
                     /locus_tag="ACHJ12_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO4992979.1"
                     /translation="ERFTRLSKNAVGENSESLRAREDVATVKKYLGNRHKLYRAGITF
                     LLRAEDMESLKRRRVELTTVLLGAGLQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQW
                     YTRLMWVQHLAGLLPVTGRETGTGHPGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFG
                     PTGAGKSATLCGSLSQIMAVHRPRLFIAEAGNSFGLLADYFESLGLSVNKISVKPGTG
                     VCLPPFADAHQLVEQGETLQSVDEHSLPDLDEDEGDEEEEKRDILGEMEISARMMITG
                     GDPKEEAALKRADRAMIREALLMATHTTYREGRQMLPVDLQSALWEISRDTQRNDVRR
                     AKAAEMAESLGMFTQPGSFEAELFNREGKL"
BASE COUNT          238 a          251 c          373 g          230 t
ORIGIN      
        1 agagcgcttc acgaggttgt cgaaaaatgc ggtcggggag aacagtgaat ccctgcgtgc
       61 ccgggaggat gtggcgaccg tgaagaaata tctgggaaac cggcacaagc tctaccgtgc
      121 cgggatcacg tttctgctgc gggcagagga tatggagagt ctgaagcgca gacgggttga
      181 actgacgacg gtgctgctgg gcgcgggctt acagccagtg cgcccggagt ttgatgtcgg
      241 accgctgaac agctggctgc gcgcgctgcc catgtgtttt gatcctgata cggataaaaa
      301 acagtggtac acgcgtctga tgtgggtaca gcatctggca gggctcctgc cggtgacggg
      361 aagggaaacc ggtacggggc atcccggatt cagtttcttt aaccgtggcg gggatgtgct
      421 gacctttgat ccgctgaaca agctggaccg gacccagaat gcacatctgc tgcttttcgg
      481 gcctaccgga gccggtaaat cggccacgct gtgtggttcg ttgtcgcaga taatggcagt
      541 tcatcgtccc cgtctgttta tcgctgaggc cgggaactcg tttggtctgc tggcggatta
      601 ttttgagagt ctcgggcttt cggttaataa aatcagtgtc aaaccgggaa cgggggtgtg
      661 tcttcctcca tttgcggatg ctcatcaact ggtggagcag ggagagacgc tgcagagtgt
      721 ggatgagcat tcactgcccg atcttgatga agatgagggg gatgaagagg aagagaaacg
      781 cgacattctg ggggagatgg aaatctctgc ccgcatgatg atcaccggag gggatccgaa
      841 agaagaagca gcgctcaaac gtgcggacag ggcgatgatc cgtgaggcac tgctgatggc
      901 cacgcacacc acgtaccggg aggggcgtca gatgctgccg gtggatctgc agagtgcgtt
      961 gtgggaaatt tcgcgggaca ctcagcgtaa tgatgttcgc cgggctaaag ctgctgagat
     1021 ggcagagtcg ctggggatgt tcacccagcc gggcagtttt gaagcggagc tgtttaaccg
     1081 ggaaggaaaa ct
//
LOCUS       DAZFFI010000386         1072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFFI010000386 DAZFFI010000000
VERSION     DAZFFI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.389"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..559
                     /locus_tag="ACHJ12_005377"
     CDS             <1..559
                     /locus_tag="ACHJ12_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4992980.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
     gene            556..>1072
                     /gene="ybfC"
                     /locus_tag="ACHJ12_005378"
     CDS             556..>1072
                     /gene="ybfC"
                     /locus_tag="ACHJ12_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4992981.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNES"
BASE COUNT          355 a          200 c          238 g          279 t
ORIGIN      
        1 ggggaggtat atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta
       61 tccgctgaat ccggttcagt atatagattc aatgggactg gcatcaaaat atggacactt
      121 aaataatggc ggatatggag cgagacccaa caaaccgcct acgcccgatc caagtaaatt
      181 gccggacata gcgaaacaat taagactgcc atatcctatt gaccaggcca gtagtgcgcc
      241 taatgttttc aaaacattct tcagagcatt aagcccttac gactacacac tgtattgcag
      301 gaagtgggta aaaccaaatc tgacttgtac gccacaggat gattcccagt atccagggat
      361 ggatacaaag acagcaagtg attacctgcc acagacaaat tggccaacaa ctcaattacc
      421 accaggatat acttgtgcag aaccctattt attcccagac attaataaac ccgatgggcc
      481 agcaacagca gggatagatg atttgggtga aattttagct aagatgaaac agagaacatc
      541 gagaggaata agaaaatgaa aagagttttg ttctttttgc tgatgatatt tgttagtttt
      601 ggtgttatag ctgattgcga aatacaagct aaagatcatg attgttttac tattttcgct
      661 aagggtacta ttttttccgc gtttcctgtc ttaaataata aagccatgtg gagatggtat
      721 cagaatgagg acatcggtga gtattattgg caaacagaac tgggtacatg taaaaataat
      781 aaattcacac caagcggggc gagactctta attcgcgtcg ggtcactacg ccttaatgaa
      841 aatcatgcca tcaaaggaac gttgcaggag ttgataaata cagcagaaaa aacggcgttt
      901 ctcggcgatc ggtttagaag ttatataaga gcgggtatct atcaaaaaaa gagcagtgat
      961 ccagttcagt tgttggcagt ccttgataat tcaatcatgg taaaatattt taaagatgag
     1021 aaaccaactt atgctcgaat gacagcacat ctcccgaaca aaaatgagtc tt
//
LOCUS       DAZFFI010000387         1060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFFI010000387 DAZFFI010000000
VERSION     DAZFFI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.390"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..749)
                     /locus_tag="ACHJ12_005379"
                     /pseudo
     CDS             complement(1..749)
                     /locus_tag="ACHJ12_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
     gene            complement(780..>1060)
                     /gene="ybfB"
                     /locus_tag="ACHJ12_005380"
     CDS             complement(780..>1060)
                     /gene="ybfB"
                     /locus_tag="ACHJ12_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO4992982.1"
                     /translation="LLILFFSMHRLSLLDSTRDVSELISLMSYGMMVICFPTGIVFFI
                     ALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLSKRIINK"
BASE COUNT          235 a          299 c          267 g          259 t
ORIGIN      
        1 atttttcgcg ccggcgtgta caccgggtcc atctggtttt gcatctgctc caccgtcagg
       61 ccgcacgatg ccagccagcg gcggctttcc tcactcaccc ggtcagccag gatttcactt
      121 tccagccggt cgagcatctg caccagcacc ggcgggaaca ccacactgcc accgtcttcg
      181 ccgccggact gctgaagggt atccgccagg ctgcggcgct gcgttttcgc ctgctcaccg
      241 gtggcggttt caaccctgat gagtggcgtg aagctccccg gctgataaat cgtctggatg
      301 cggctcctgt cgttctgtat cgtggtcagc cggtcgccgt cccagccgta ccaggtcact
      361 tgcggtttcc gtgacagcga catccagccc gtcaggtccc gttcacgtcg ccacacccgt
      421 tttgccaccc tgcggcccag cgggtcgtaa agatagcggc tttcgaccag cggctctgca
      481 tattgtgtcc gcgtgtagtg caccagccgg tgctgactgt cgtaatggta ccggtgggtg
      541 cgctcatcat ccgtgcggat aaccccttcc gggatgaggt cggttttctc cgtcagcctg
      601 ccgtgacggt cataccggta aagatagtgc gcgtcacggg cgatacggtt atccggccac
      661 atgctgaggg cgctgtccgg gtgcagctcc gggtcgggca ggcggttacc tgccgggtct
      721 gtggcatacg ggatatcaat gcccaggtta catataataa taagagcaat taacttaatt
      781 tatttgttta taatgcgctt acttaataca aaccattgaa tataccctcc tgacagaaaa
      841 taaagccata ttattatcgc cataatatac ttactatcaa tccttacgcc aataatgtct
      901 gatacagtcc ctataaatat tagagcaata aagaatacta tgcctgttgg aaaacatatt
      961 accatcattc cataagacat gagactaatt aactcgctca catcacgagt ggagtctaat
     1021 agtgaaagtc tatgcatgga aaagaatagt attaaaagcg
//
LOCUS       DAZFFI010000388         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFFI010000388 DAZFFI010000000
VERSION     DAZFFI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.391"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..309
                     /locus_tag="ACHJ12_005381"
     CDS             <1..309
                     /locus_tag="ACHJ12_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4992983.1"
                     /translation="AGFKGIIPAKITSRSQCCDDDAMVICGCIARLYRNNRDLHDLLV
                     DYYVLGETFMALARKHGCSDTCIGKRLHKAEGVVEGMLMMLDVKLEMDRYVERDSVTA
                     "
BASE COUNT          318 a          247 c          266 g          228 t
ORIGIN      
        1 gcaggattta aggggattat tccggcaaag ataacatcac gctcacagtg ctgtgacgat
       61 gatgcgatgg tgatatgcgg gtgcatagcc cgtctttacc ggaacaatcg cgatctgcat
      121 gacttgctgg ttgattatta cgtgttgggg gagacgttca tggcgctggc acggaaacat
      181 gggtgctctg acacctgtat aggtaaacgc cttcacaaag cggagggggt tgttgaaggc
      241 atgttgatga tgctggatgt gaagctggag atggatcggt atgttgagcg cgattcagtg
      301 acggcataac gcgcataaaa aaaccgcagt catgaggaaa ctgcggtgga gaaatacggc
      361 ttgccaatca atcatgaaaa acgtaatcgc ctgataatta tgccacgaga caaaggacgg
      421 tgcaccctga atggtcataa tgaccactca gaccatcgag gcaaccataa cgaacatcgt
      481 tacagttgga gcttttaccc cgttggggga aaaggtgcat acctgaatca tcattatggt
      541 gatcaagggc atgtaaagcc caccagcctg attaaaggtt aaccggaaaa agccaggtat
      601 ggagggagtg attagccacg aacaagccgg acaccaccag cagaaggtat tatgattttc
      661 gtaatacctt tagcaaagcg tgaatcatca ttaccgtagg gatggtcata atgaccacac
      721 cttcccccac cggaacaagg aaggctggct tacgaaaatt tcgtaaccct ccccagcaca
      781 atgtattaaa taatcactac aggccattga ttaaacttaa ttatcgcgaa tcgcgagaat
      841 tagccccgcc ggaacaagga aagggaaatt acgaaaattt cgtaaaatcc ccccacatct
      901 cccacggttg cccgttaagt gcaagcgcac aggcacgacc tcattaacca gttaccagcg
      961 ctcacgctca aacatccaca gcaatgggga taaatcgcac tctggcgcaa tgttcactat
     1021 cggggcgagc attcaccgga aaacgtcata caggcttac
//
LOCUS       DAZFFI010000389         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFFI010000389 DAZFFI010000000
VERSION     DAZFFI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.392"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHJ12_005382"
     CDS             complement(<1..158)
                     /gene="ssb"
                     /locus_tag="ACHJ12_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4992984.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKE"
     gene            complement(174..377)
                     /locus_tag="ACHJ12_005383"
     CDS             complement(174..377)
                     /locus_tag="ACHJ12_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992985.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(468..935)
                     /locus_tag="ACHJ12_005384"
     CDS             complement(468..935)
                     /locus_tag="ACHJ12_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO4992986.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT          263 a          272 c          238 g          282 t
ORIGIN      
        1 tgttccttca tttcaccgct ctgtttatca cgccaggtat cagtggtggc cagcgtcaga
       61 tttgccactg cacctccgcc gttggggaaa taacggattt ccggatcctg tcccagatga
      121 ccaatcagta tgactttatt cactccacgc gatgccataa caactcctta tcctcacaca
      181 cattcacgct gagtccagat gccgttttcc agagcccgga ctgcctgctc ataatttctg
      241 aaatactctt cggattcccg ggacacaggc ccttcgtcgc actgagtacc aatataataa
      301 ccacgagcac tgctgaggat ctgcagaggt aaggtatagc ccgctttggc ggccagaatt
      361 ccgcttgtcg gtttcataac atgtttcctt aaagttaaac gataagaaaa cagacctccc
      421 tgatggttgg tctgttttca tcagggaggt tgagttgaaa aatacgatca gaaagaattt
      481 tctgcgtatt cccgcggggt attgccattc tgtggcggta tggattccga tttttcagtc
      541 ttataaacca tgtcctggcc tattttgatc cactccacct tgatcaggcg ggctttcaga
      601 ctgacgcgtt gctcaccggc atgttcaccc cggttaaggg taaaaatgtc agtagagggg
      661 ttgctcatgg taaaaccaat cagcaccttg cgatcttcat ccaccgcttt ctggcagcgg
      721 gcaatcaggg aagttgcctc tttaccggca accgtcacat caaaacgcac ataggacggg
      781 ctgtccgtcg gaccactcag tgcattcaca acacaactga gaaatgtccc gttctgatga
      841 ttaacctgac gaatgttgct gagatacccc aggcctttca gtgtcagatt gaagtactca
      901 gttttagcgt ttgcagaaga agtatttgca gtcataatga tatctccatt ctcagtatcg
      961 gaaagacaga gaacatcatc atccctgacg ggttgtgata ttcccgtcag ggagccggaa
     1021 cagaactatt ccggctgact cagtaacagt taaag
//
LOCUS       DAZFFI010000390         1047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFFI010000390 DAZFFI010000000
VERSION     DAZFFI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.393"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1002
                     /locus_tag="ACHJ12_005385"
     CDS             <1..1002
                     /locus_tag="ACHJ12_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4992987.1"
                     /translation="VSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFLTGHHTLF
                     MSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFST
                     LSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADA
                     VKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPAL
                     DCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGAAAGVFGN
                     ATGGRRGAILGAFAQGLLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
BASE COUNT          210 a          229 c          276 g          332 t
ORIGIN      
        1 gtttccgtag cgcagaaaag cttcgggaaa gaaatggcga tgattatgtt ctttgcgatg
       61 gttatcaaca ttatgattgc ccgtttcaca ccgtggaaat ttatcttcct gaccggtcat
      121 cacacgttgt ttatgtcgat gatggtagcg gtaattctgg caacagcagg catgaccggc
      181 attacgctta tagccgttgg ctctttggtt gttggcgtgg caatggtctt tttcccggct
      241 attgcgcatc cgtacatgaa gaaagtgacc ggttctgatg acgttgccat cggccacttc
      301 tcaacattat cttatgtatt ggccggtttc atcggcagca agtttggtaa taaagagcac
      361 tcgactgaag acatgaatgt gccgaaaagt ctgctgttct tgcgcgacac gccggttgct
      421 atctctttta ccatgagcat aattttcctg gtgacgtgcc tgtttgcggg tgcagatgcg
      481 gtgaaagagc ttagcggtgg taaaaactgg ttcatgttct ctatcatgca atccattacc
      541 ttcgcagctg gcgtgtacat cattctgcag ggtgtgcgca tggtgattgc ggagattgtc
      601 ccggcattca aaggtatttc agacaagctg gttccgaatg ccagacctgc tctcgattgc
      661 ccggtcgtct tcccttacgc acctaacgct gtactggttg gtttcctgag tagctttgct
      721 gcaggtttaa tcggtatgtt cacgttgtat ctgctgaaca tgatcgtgat tattcctggt
      781 gtggtgccgc acttcttcgt aggtgcagct gcaggggtat tcggtaatgc aacgggtgga
      841 cgtcgtggtg cgattttggg tgccttcgct cagggcttgc ttattacctt cctgccagta
      901 ttcttattgc ctgtgctggg taacattggt tttgctaaca ccaccttcag tgatgctgac
      961 tttggcgcac tgggtatttt gttagggatt atcgttcgct aatattttga tgacattaat
     1021 atcctccagg atttcaggaa cgtggag
//
LOCUS       DAZFFI010000391         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFFI010000391 DAZFFI010000000
VERSION     DAZFFI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.394"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..483
                     /locus_tag="ACHJ12_005386"
     CDS             <1..483
                     /locus_tag="ACHJ12_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4992988.1"
                     /translation="QKLYEGWYQLRIAGSDVSEQELHSRVCEPLNDGDVIHLVPRTEG
                     AKNGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMMVGGITQMLTPRAKTPSATT
                     TDNGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG
                     "
BASE COUNT          281 a          205 c          311 g          248 t
ORIGIN      
        1 cagaaactgt atgagggatg gtatcagtta cgcattgcgg gcagtgatgt atctgaacag
       61 gagcttcatt ccagagtatg tgagccgttg aatgacgggg atgttatcca tctggtgccc
      121 cggacggagg gggcaaaaaa cggcggtgtt cttcaggtgg tggccggtgc tgttctggct
      181 gttgtgggat atgtgttcag ctggaccggt attggtgctg tcatcggtaa tcttggggtg
      241 gccatgatgg tggggggtat cacgcagatg ctcaccccca gggcgaaaac accgtctgcc
      301 acgacaacag ataatggtaa acggaacacg tatttctcct cactggacaa catgattgcc
      361 cagggtaacc cgatgccggt gccttacggt gaaatgctgg ttggttcacg acggatatcc
      421 caggacatca gcacccgtga tgagggcggt gacgggaaag tggtggttat cggacgggga
      481 tgaaaataaa aaaatcccgc agagttagcg gagctgcggg aaataacgat gaagattaac
      541 gttatggagt tatttttttg ggcagaagaa cagtaacgca gcgtcattat tgcggctaca
      601 ggcaattgcc ggaagtgtga ataaactcag aatttttatt ccgttgtggt gatgcccggg
      661 ctggccagtg acgggataaa aaaatcccgc agagtccgga gactgcggga cagaaccgat
      721 gaagattaac gttcaaggag ttttgttttt attgacccga aaaaactgta acgccccgga
      781 atgatatctg ccacgggaac gtacagaaaa tgtgaagaaa ttcagacttt ttctgtcgca
      841 ggtttacaga acggatgacc gccagggtac cagtggcgta aatgagcagg atgttgtggt
      901 gtggctgaag caaaaaaatc tcccgtaata tcgccggata ctgacgggag acgaatagtc
      961 accttaagga ggtctgataa ttattgcttt tattgagcaa aaaaactgta acgctgcgtc
     1021 attatagtgg ctacaggcaa ttgcc
//
LOCUS       DAZFFI010000392         1044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFFI010000392 DAZFFI010000000
VERSION     DAZFFI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.395"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1044
                     /locus_tag="ACHJ12_005387"
                     /pseudo
     CDS             1..1044
                     /locus_tag="ACHJ12_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          252 a          245 c          326 g          221 t
ORIGIN      
        1 cacagacggt ggaacaactg aagatgaccg ctgaaattgt ctgttcggtg ttccgtgtcc
       61 cggcctacaa gattggcgtg ggacaaccgc cttccagtga caacgtggag gcgctggagc
      121 agcagtatta ttcccagtgc ctgcagacac tgattgagtc cattgagctg ttactggatg
      181 aggcgctgga aacgggggaa aacgagagta cggaatttga tgtcaccacg ctgctgagaa
      241 tggacagtga gcggcgcatg aaaacgctgg gggatgcggt gaaaaatacg cttctcacgc
      301 ccaatgaagc ccgaaaacgg gagaacctgc cgcccctggc aggaggcgat gcactgtatc
      361 ttcagcagca gaactacagt ctggaagcgt tgtcccgccg tgatgcccgt gaggatccgt
      421 tcgcatcgtc cggtaaaaca gcttccgtgc ctcaggcggt cgttgcctct gacggtaata
      481 aggcaatcac tgaaacagag catgacgcgg tgaaggcgat gttcaggggg attctgaaaa
      541 aatgaatgaa cgtgaactgt ccattatccg tgcgctgggt gaagaatttt ccgcggtgct
      601 ggcggattta cagtgcacat ttgaggagaa aatagccgcg caggcacaaa cgtttgaaga
      661 aaaactggct tccctgtctg tggtattaca gaagtgcgtg acgggtgatg atgtgcgtcc
      721 gatgcttgaa cagatagtga aggaggcggt aagccatatt cctgttccgc gtgacggtcg
      781 tgactacgat ccggaagtac tgcagaaggc ggtgaatgat gcggtcgcaa atattccgca
      841 gccggcagac ggtaaaagtc tcaccccgga tgatgtgcgt ccgatgcttg aacagatggt
      901 gaaggaggcg gtaagccata ttcctgttcc gcgtgatggt cgtgactatg atcccgatgt
      961 tctgcagaag gctgttctgg atgcggtgag tgccctgccg gctccgcagg acgggcgtga
     1021 tgccacggca ctggaaatac tccc
//
LOCUS       DAZFFI010000393         1042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFFI010000393 DAZFFI010000000
VERSION     DAZFFI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.396"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            494..823
                     /locus_tag="ACHJ12_005388"
     CDS             494..823
                     /locus_tag="ACHJ12_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992989.1"
                     /translation="MSEITVKNISPAVAGWWAKFRDDDGTEWYSPIAAWALCEVDYFG
                     AGNTRREILPVLTSELGMGPHPSDEGYCACLYLPDEKFTLSGEQGSFAWYPVNSNSNN
                     GNSGAES"
     gene            820..>1042
                     /locus_tag="ACHJ12_005389"
     CDS             820..>1042
                     /locus_tag="ACHJ12_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992990.1"
                     /translation="MTAKHTKNHNSVVKFVSARGRPTLLVPGKHLHYCDENHIPIVIV
                     WKRTVYADVTWLYDSLALMHRDLFEREEFR"
BASE COUNT          284 a          220 c          282 g          256 t
ORIGIN      
        1 cggaatgctg aaaagaccat ccagcggtcg tgggtggcaa atcaggacac cacggctaaa
       61 acacctgaaa ggggcgcgcc tgagagtgta cggccttttg ctggcgcagg accacgacac
      121 ggaaagcgat taatctcgcg tataggcgat gaaaaaatgt tgtcccagtt gtcccactac
      181 gtattaattg attgatttta ctaaaaatac aataaaaaag tgggacaaca ttacagcatt
      241 ttgggacgac atcggggtat tttgggacga catagcgaca ttttgggacg acacaacgag
      301 cgttaattac cctggctggt gactgggaca acataaaacc cctcatgttg tcccaaaatc
      361 ggctaatgtt gtcccagttc tcggagtgta tttttaatta aatcaatgga ttatgtataa
      421 gtgggacaac tgggacaact gggacgacgt gtttttgtat atatataaaa aaataaattg
      481 agaggatgaa ttcatgagtg aaattactgt taaaaatatc tcacctgcgg tagctggctg
      541 gtgggcgaag ttcagagacg acgacggcac ggaatggtac agcccgattg ctgcatgggc
      601 gctttgtgag gttgattatt tcggtgcggg gaacacccgc cgcgaaatct tacccgtgct
      661 gaccagtgag cttggtatgg gtccgcaccc atccgatgaa ggctattgcg cgtgtctgta
      721 tctgcctgat gaaaagttca cgctctcagg tgagcagggt tcttttgcct ggtatccggt
      781 gaacagcaac agtaataacg gcaattcggg ggctgaatca tgacggcgaa gcacacaaaa
      841 aaccataatt ccgtggtgaa gttcgtatct gcccgtggca ggccaacgct tttagtgcca
      901 ggaaaacatc tgcattactg cgatgaaaat catatcccga tcgtgattgt ctggaagcga
      961 acggtatacg ccgatgtgac atggctgtat gactcgctgg cgttgatgca ccgtgacctg
     1021 tttgagcgtg aagagttccg gc
//
LOCUS       DAZFFI010000394         1039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFFI010000394 DAZFFI010000000
VERSION     DAZFFI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.397"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1039
                     /locus_tag="ACHJ12_005390"
     CDS             <1..>1039
                     /locus_tag="ACHJ12_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4992991.1"
                     /translation="GTIKLTSSPAANGKSASATIAVNAGKGGLTDAAGTTKTRLTYAP
                     DGTLPAEELATMNDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEK
                     GDGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQGNTVSLTGSGLNN
                     GGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGS
                     KVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGTRMDKNGLTI
                     KDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTWALKTESGDE
                     AVAVSSQTVEVR"
BASE COUNT          275 a          231 c          309 g          224 t
ORIGIN      
        1 atggcacgat taaactgaca tcctcgcctg ctgcgaacgg aaagtctgcg tcagcaacca
       61 ttgctgttaa tgcaggcaaa ggtggattaa cagatgctgc cggtaccacg aagacacgtc
      121 tgacttatgc tccagatgga acattaccgg cagaagagct ggcgaccatg aatgacggtc
      181 tttcttttga gggtgatgac ggaaagatta ttcataaaaa actgaatgaa aaactgacca
      241 tcaaaggtgg ggctggtgat aatgaggatg taacagacag taacattcgt gttgatgcaa
      301 atgaaaaggg agatggtctt ctcgtccgga tgaccagaaa cctccgtgat ttggccagcg
      361 cggtttttgg tacaggtggg cagataacaa cagttaatga taaaggcatc acaatcacca
      421 caccggctgc gggcggtggt caggggaata cggtttccct taccggctcc ggcctgaata
      481 acggtggaaa tcagataacc agtgtggcca gtggtctgaa cggcactgcg cttgctgacg
      541 ccggtggaga tgttctgaat aatgcggcca atatcggtga cctgaaaaat gctctccgtg
      601 acagcagtgc ggcactggtg aataccggct ttggtctgca ggcggatgac ggaagcaagg
      661 tgaacacgtc tctcgggaag catatcacgg ttggtggtga cgggcagaat atcagcacga
      721 ctgttcagaa cgggagcctg acggtcagac tcgctgataa cattcgcctg gcagatgacg
      781 gcagtgtttc tgttggcgga acccggatgg ataaaaacgg actgaccatc aaggatggcc
      841 cgtcagtgac tgtgaacggt attgatgctg ctggccgggt tatttccggc gtgaaagacg
      901 gggtgaatga ccaggacgcc gttaacgttt cacaactgaa ccgtgaaatc agtcaggcaa
      961 cggcagcaac gacctgggca ctgaagactg aatccggtga tgaggctgtt gctgtttcct
     1021 cccagacagt tgaagtccg
//
LOCUS       DAZFFI010000395         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFFI010000395 DAZFFI010000000
VERSION     DAZFFI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.398"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1036
                     /locus_tag="ACHJ12_005391"
     CDS             <1..>1036
                     /locus_tag="ACHJ12_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4992992.1"
                     /translation="GTIKLTSSPAAGGKSASATIAVNAGKGGLTDAAGTTKTRLTYTP
                     DGKSPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNAEVTDGNIRVDNDNG
                     GLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTPAAGGGQGNTVSLTGSGLNNG
                     GNQITSVASGLNGTALADASGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGSK
                     VNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLGDDGSVSVGGTRMDKNGLTIK
                     DGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTWALKTESGDEA
                     VAVSSQTVEVR"
BASE COUNT          270 a          235 c          314 g          217 t
ORIGIN      
        1 atggcacgat taaactgaca tcctcgcctg ctgcgggtgg aaagtctgcg tcagcaacca
       61 ttgctgttaa tgcaggcaaa ggtggattaa cagatgctgc cggtaccacg aagacacgtc
      121 tgacttatac cccggatgga aaatcaccgg cagaagagct ggcgaccatg aatgacggtc
      181 tttcttttga gggtgatgac ggaaaggtta ttcataaaaa actgaatgaa aaactgacca
      241 tcaaaggtgg tgcagagaag aatgctgaag tgactgacgg caatatccgg gttgataatg
      301 ataatggcgg gcttctggtg aggatgacca gaaacctccg tgatttggcc agcgcggttt
      361 ttggtacaga aaacggcccg acaacccgga ttgacaatgc aggcatcaca atcacaacac
      421 ctgctgcggg cggtggtcag gggaatacgg tttcccttac tggctccggc ctgaataacg
      481 gtggaaatca gataaccagt gtggccagtg gtctgaacgg cactgcgctt gctgacgcca
      541 gtggagatgt tctgaataat gcggccaata tcggtgacct gaaaaatgct ctccgtgaca
      601 gcagtgcggc actggtgaat accggctttg gtctgcaggc ggatgacgga agcaaggtga
      661 acacgtctct cgggaagcat atcacggttg gtggtgacgg gcagaatatc agcacgactg
      721 ttcagaacgg gagcctgacg gtcagactcg ctgataacat tcgcctggga gatgacggca
      781 gcgtttctgt cggcggaacc cggatggata aaaacgggct gaccatcaag gatggcccgt
      841 cagtgactgt gaacggtatt gatgcggctg gccgggttat ttccggcgtg aaagacgggg
      901 tgaatgacca ggacgccgtt aacgtttcac aactgaaccg tgaaatcagt caggcaacgg
      961 cagcaacgac ctgggcactg aagactgaat ccggtgatga ggctgttgct gtttcttccc
     1021 agacagttga agtccg
//
LOCUS       DAZFFI010000396         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFFI010000396 DAZFFI010000000
VERSION     DAZFFI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.399"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..759)
                     /locus_tag="ACHJ12_005392"
     CDS             complement(61..759)
                     /locus_tag="ACHJ12_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4992993.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYALFLDAVNFVAGNPEADPEQELTDRWVVEQLSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            complement(759..>1034)
                     /locus_tag="ACHJ12_005393"
     CDS             complement(759..>1034)
                     /locus_tag="ACHJ12_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4992994.1"
                     /translation="SVREVRFGDGYSQRMAAGLNADLKTYRVTLSVTREEARHLEAFL
                     AEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT          218 a          369 c          238 g          209 t
ORIGIN      
        1 ggcgcacatc gccgggcatg cgccagaatc gctgattcag tctgtgtcat aaaccgggat
       61 ttactgcgaa agtttgttaa tggaaaggaa accgccaaaa ttgaccgcca tgccgcgcat
      121 ctcacacccg cgcatgcatt tactgcatct gtccttccgg atatccgtgg tggggttgtc
      181 gaactcatcc gccactgccg gaccgttata cccgcactca tcgccccggt aatcccacat
      241 acaggtgttc gccagcatga tgcgaccggg aaacagcgca ccgtccgtct ccgtcggtgt
      301 cgccagcaca aacgaggccg tcatggccgt cagctctgat aactgctcca ccacccaccg
      361 gtccgtcagc tcctgctccg ggtctgcctc aggattgcct gccacaaaat tcaccgcatc
      421 cagaaaaagc gcatacaccc ggcgacggac caccgtggca cccaccaggc tctgcaaatc
      481 ctccgccatc ccggtgacca gtccgaaaag attggacact gtcagcgacg ggcgggcact
      541 gctgcccttt ccgttcatct caaagccgct gccctcaatc gggtatgcct gatattgccg
      601 cccctgccag gtcaccggct cccctttttc attcagctca ttgcagaaaa aataccgctc
      661 accgccctgc accgtcaggt cgatttccca gagcaccacc cgcggtgact gctctgactt
      721 aaccgactcg ttcaggcttg cttcgtgaat atcctgcatc agttcaccac ctgcttaaac
      781 tccgcgctga actcaacgcg caacatcccg acccgcgcag accacccggc gcaggtcacc
      841 tttatctgcc ggtatgcata gggtggcttc cacaaaaatg ccttccagcc cccgtgctct
      901 gccagaaacg cttccagatg ccgggcctcc tcccgggtca cggaaagcgt caccctgtat
      961 gttttcaggt cagcattcag ccctgccgcc atacgctgtg agtacccgtc accaaaacgc
     1021 acttcacgca ccga
//
LOCUS       DAZFFI010000397         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFFI010000397 DAZFFI010000000
VERSION     DAZFFI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.400"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..395)
                     /locus_tag="ACHJ12_005394"
     CDS             complement(<1..395)
                     /locus_tag="ACHJ12_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO4992995.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVW"
     gene            complement(402..743)
                     /locus_tag="ACHJ12_005395"
     CDS             complement(402..743)
                     /locus_tag="ACHJ12_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4992996.1"
                     /translation="MSRIYGRMKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(748..>1031)
                     /locus_tag="ACHJ12_005396"
     CDS             complement(748..>1031)
                     /locus_tag="ACHJ12_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO4992997.1"
                     /translation="TALLMRRMLLTGMAEPYAAAQPAALEEGERRIEALDREIMALKN
                     EMELKRDLARNSVLTIIERDNERVGNNPMIQQADNADSRIHSLDAPDKE"
BASE COUNT          250 a          340 c          217 g          224 t
ORIGIN      
        1 caccataccg gggctgcagc agtctgatcc cctatactgc tgatcacccc actgtacccc
       61 gtctctccgg gctcttcagg tgtccatacc ccacaggtct gtgccctgga tttatcgaat
      121 gacagcgtat ccagcgtcac ctgtaccaga ggaacacagc aggatatcat cacaaaaaaa
      181 gcaccataga gaacatgctc aatacgcggc agggaaagca tccctttgtt tccctcatcc
      241 gcaccttctt ccctgacttt cagccagatc ccgatgatcc tgaatgccag tggcagggca
      301 aacagtcctg tgctgatcaa cacattccac agaccgttat tcaccaccca gcccagcaga
      361 gtcagaaaat attccagata gttatttgtg gtcatgattg ctcaacgccc cggctcagca
      421 aaaatggcgt attcacacag caggataaac agaacactga ctgtggccat acgcagaaaa
      481 gaagcccggt attctgccct gaaccccggc gcatgccaga tcctccaccc cagccatccg
      541 atcgcactgt aaagacacag acgccatccc agccagccat aacgtgtctc atacatccac
      601 tcccgcaggg ccgcaaatcc ttctgcgtga cgtataccgg tatcggccag ccagagggaa
      661 aacacggtaa tccctgtcag caacagcgta tacagggcga ataccttcat cctctttttc
      721 atccggccat aaattctgct catcctctca ctccttatcc ggggcatcga gagaatggat
      781 acggctgtca gcattgtcag cctgctggat catcgggtta ttccccacac gctcgttatc
      841 ccgttcaatg atggtcagaa cactgttacg cgccagatcc cgcttcagct ccatctcgtt
      901 tttcagcgcc ataatttccc gatccagcgc ctcaatacgg cgttccccct cttccagtgc
      961 agcaggctga gctgcagcat acggctcggc catccccgtg agcagcatcc tgcgcatcag
     1021 cagcgccgtt t
//
LOCUS       DAZFFI010000398         1030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFFI010000398 DAZFFI010000000
VERSION     DAZFFI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.401"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..895
                     /gene="gadX"
                     /locus_tag="ACHJ12_005397"
     CDS             71..895
                     /gene="gadX"
                     /locus_tag="ACHJ12_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4992998.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          305 a          190 c          228 g          307 t
ORIGIN      
        1 caatttgact taagagggcg gcgtgctaca ttaattaaca gtaatatgtt tatgtaatat
       61 taagtcaact atgcaatcat tacatgggaa ttgtctaatt gcgtacgcaa gacataaata
      121 tattctcacc atggttaatg gtgaatatcg ctattttaat ggcggtgacc tggtttttgc
      181 ggatgcaagc caaattcgag tagataagtg tgttgaaaat tttgtattag tatcaaggga
      241 tacgctttca ttatttctgc cgatgctcaa ggaggaggca ttaaatcttc atgcacataa
      301 aaaaatttct tcattactcg ttcatcactg tagcagagat attcctgttt ttcaggaagt
      361 tgcgcaacta tcgcagaata agaatcttcg ctatgcagaa atgctacgta aaagagcatt
      421 aatctttgcg ttgttatctg tttttcttga ggatgagcac tttataccgc tgcttttgaa
      481 cgttttgcaa ccgaacatgc gaacacgagt ttgtacggtt atcaataata atatcgccca
      541 tgagtggaca ctagctcgaa tcgccagcga gctgttgatg agtccaagcc tgttaaagaa
      601 aaaattgcgc gaagaagaga catcatattc acagttgctt actgagtgta gaatgcaacg
      661 tgctttgcaa cttattgtta tacatggctt ttcaattaag cgagtcgcag tgtcctgtgg
      721 atatcacagc gtgtcgtatt tcatttacgt ctttcgaaat tattatggga tgacgcccac
      781 agagtatcag gagcgatcgg cgcagggatt gccgaaccgt gactcggcgg caagtattgt
      841 tgcgcaagga aatttttacg gcactgaccg ttctgcggaa ggaataagat tatagaattt
      901 tactcagaca taaaaaaaac ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc
      961 agactaataa accgttataa cactccctgt tggcacggga aactttgtgc tctcagtaag
     1021 ttaaatataa
//
LOCUS       DAZFFI010000399         1020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFFI010000399 DAZFFI010000000
VERSION     DAZFFI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.402"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..413
                     /gene="ycgZ"
                     /locus_tag="ACHJ12_005398"
     CDS             177..413
                     /gene="ycgZ"
                     /locus_tag="ACHJ12_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO4992999.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            456..728
                     /locus_tag="ACHJ12_005399"
     CDS             456..728
                     /locus_tag="ACHJ12_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO4993000.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            757..>1020
                     /locus_tag="ACHJ12_005400"
     CDS             757..>1020
                     /locus_tag="ACHJ12_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFO4993001.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          349 a          166 c          204 g          301 t
ORIGIN      
        1 aaatcttgta tagattgtca gttaaatgat gcaatatgtt ttatcataac acattgtttt
       61 atatgcatta gcactaattg caaaaaatta atttatcatt ctgtacacat atttcgtaca
      121 agtttgctat tgttacctca cttcacattg attaacattt ttaacagagg cgtagcatgc
      181 atcaaaattc agtgacttca gattctgcgg gagcaatcac tcgttacttt gcaaaagcta
      241 acttgcctac tcagcaggaa actctcgggg aaattgtgac tgagattttg aaagatggac
      301 gtaatctgag tcgaaagtcg ctttgtgcca aacttctttg ccgactggaa caagcgacag
      361 gggaagagga acagaaacat tataatgcac taattgggtt gctttttgaa taatgcaaca
      421 gcctgattat ttaacaggtt agctatgaag tcgttatgaa gacatctgat aatgaacgta
      481 taaaatatga aattactggc caggcggtgc tccagatact gcgcatgaag ataaattttt
      541 cattgcagac tcttattaag caattactcg taatgaaatc ggctgaggaa gatgctttcc
      601 gacgcgattt aattgacagc ataattcgtg attttagcaa tagtgattca ggagggccaa
      661 accgaagaac agcgacagct gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa
      721 tacattaaaa tttcataagt aagatgagag gttaccatgc ttgaagatac tacaattcat
      781 aatgcaataa ctgataaagc gttagcaagt tactttcgca gttcgggtaa tttgttagaa
      841 gaagagtcag cagtgttagg gcaggctgtc accaatttaa tgctttcagg cgataatgtt
      901 aataataaaa atattatctt aagtctgata cactccctgg aaacaacaag tgatattctc
      961 aaagctgatg tgattagaaa aacactggaa atcgtgttgc gatacacagc tgatgatatg
//
LOCUS       DAZFFI010000400         1007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFFI010000400 DAZFFI010000000
VERSION     DAZFFI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.403"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..319)
                     /locus_tag="ACHJ12_005401"
     CDS             complement(<1..319)
                     /locus_tag="ACHJ12_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO4993002.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQ"
     gene            500..652
                     /locus_tag="ACHJ12_005402"
                     /pseudo
     CDS             500..652
                     /locus_tag="ACHJ12_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(677..>1007)
                     /locus_tag="ACHJ12_005403"
     CDS             complement(677..>1007)
                     /locus_tag="ACHJ12_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993003.1"
                     /translation="RFQFIIYTLTSWSILITLNDINLALSFILVINNTYVNINIGAKR
                     IRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSA
                     KMSLSAH"
BASE COUNT          326 a          174 c          189 g          318 t
ORIGIN      
        1 tttgtccaaa aaaggaaaac atttttgcta gttctttcat ttctgcaaat aaaatatcag
       61 tgcattcacg ttgtgatatt tttgttccgg aacttgaggc actaaaagca acattaatag
      121 aacctgggcc agggaaatgg tatttaataa gtctgtaagg atcaactttt gctgagatat
      181 cgcttaggcc gagtgagaga aagtcttgtt ctaccatatc accatattgc atatcatcag
      241 cagaaaaatc atcgaataaa ctaatagttt cgaaaagtgt acagggaata ttaattggcg
      301 gaagtaatat gttattcatt tgcatttgcc ttggttatat ttaagctcaa tatcaattcg
      361 agaacaaatg ctagggtaaa aaaatattta aaaaagaagt aaacttcaca ttttttcatt
      421 acatctctga agagaacctc gttaatgatt ggtcttgctc attgttaata acaaaatgtt
      481 gaatttaccg attaggaaac tttttcctac gcggattgcc tccagctatg gcatgaactt
      541 tgagtttatg ccagaactga agtggggctt cggctaccct ggcgcgatta tctttatgat
      601 cctcgcgggc ctgacaccgt atctgtactt taagcgtaag aactggctgt aactatacga
      661 tgtcgctgtg agtaagttag tgtgctgaca gcgacatttt agccgaggga atcgcagata
      721 acataattgt tgtgaataat atccatgcca cgttgagttg gacgcttaat gactcacatc
      781 tcaccccttc aattgctgca gagataatag aaaaaagtgt caagcccaat gaaatcaata
      841 caaagtagat tgccttacat ccagtatctc ttattctttt tgctccaata ttgatattta
      901 cataggtatt attaataacc agaataaaac tcaaagccag gttgatatca ttcaaagtta
      961 ttaatattga ccatgatgtt aatgtataga ttatgaactg aaagcgt
//
LOCUS       DAZFFI010000401         1003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFFI010000401 DAZFFI010000000
VERSION     DAZFFI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.404"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..240)
                     /locus_tag="ACHJ12_005404"
     CDS             complement(<1..240)
                     /locus_tag="ACHJ12_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO4993004.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEF"
     gene            complement(233..>1003)
                     /locus_tag="ACHJ12_005405"
     CDS             complement(233..>1003)
                     /locus_tag="ACHJ12_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFO4993005.1"
                     /translation="WEQVIRKKAVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYK
                     HGMNAGLPGGNGTKNPVNLHCDEFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEA
                     RTGSAAKTAQVQGNFNNMIMLRVRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTT
                     GQDFTSSTQDRVGTVKVPLLEPADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDAL
                     MPESIAKIAEAMRRNYHTGEGWWNETPSVNMPEGEQNG"
BASE COUNT          224 a          320 c          231 g          228 t
ORIGIN      
        1 gaattccgaa gacagccacc ccagttctgt cttcatcact gcctcactgt gtgctgcccc
       61 ctgctccggc cagaaaaaag caatcccgat atactcaacc agcaggctca gcagcaggga
      121 ggaaaacagg atcccgacca gcatccacgg ccagccccac aacagattcc agagcagtcc
      181 gtgctgacgt ggcggagagg gttgtgctgc tctgttcacc tcacgacggg gttcagccat
      241 tctgctctcc ttccggcatg ttcacggagg gagtctcatt ccaccatccc tctccggtgt
      301 ggtaattacg ccgcatggcc tctgcaattt ttgcaatact ctccggcatc agcgcgtcat
      361 ctgcatcagt tgcaggcagg ggcatacgga ttttccacaa ctgtccccct tccagcagag
      421 caaaggcctg tcctttcggc agggtcacga tatctgccgg ctccagcaaa ggcaccttca
      481 cagtaccaac acgatcctgg gtactggatg tgaagtcctg gccggtcgtg acatctgcgg
      541 tatcctgatg accggaaacc agcgttttgg tataaatttc cacctgggac aactgggtcg
      601 tcagtaactc ggccgtccgg ttctccctga cccgcagcat aatcatgtta ttaaagttac
      661 cctgcacctg ggccgttttg gcagcactac cggtacgggc ctcaatatca gaagaggtct
      721 gcgtgtaagc cgtcacctgc ataccggcac cgcctccctt gttgattaac ggaatgaact
      781 catcgcccat cagttcatta aactcatcac aatgcaggtt aaccggattt ttggtaccat
      841 tccctcccgg aagaccggca ttcattccgt gtttgtagat atgcccggcg acactcacca
      901 gatccgcaaa catggagtta cccacggcac tggccacctc actgtcactc agcgcatcaa
      961 gaccaatgta caccaccgcc tttttacgta tcacctgctc cca
//
LOCUS       DAZFFI010000402         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFFI010000402 DAZFFI010000000
VERSION     DAZFFI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.405"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            229..>1002
                     /locus_tag="ACHJ12_005406"
     CDS             229..>1002
                     /locus_tag="ACHJ12_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993006.1"
                     /translation="MSKKITKTLLSSSLVVGLLSVSSFTRANEYSYSALDVLGKHGST
                     YTIVYNSDSDTAYVKWKKVPSGVEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVY
                     DREFHNLMNSLVKQDKENTLGKKEGTQTDLVSDADIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQTYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDS"
BASE COUNT          322 a          154 c          237 g          289 t
ORIGIN      
        1 acagcatgcc ggatgagagt gagttttttc tgatgcctgt tccggtggtg tagtcgtggt
       61 tctgagtgac tacaccaaga ataatgtaag ataaatccta aattattaaa ttaatttttg
      121 gatttatttt tggaatatcc tgaatgaagc cgtaattaat catatttctt tatgagtatg
      181 aggtgttgag gctgatttgt atacagataa tttaacagga tatccataat gagtaaaaaa
      241 atcacaaaga cacttctgtc atcttctctg gttgtaggtc tgttgagtgt tagttctttt
      301 acacgtgcaa atgaatatag ctacagcgcc ttggatgtgt taggtaaaca cggaagtact
      361 tacaccattg tttataatag tgattctgat accgcttatg ttaaatggaa aaaagtacct
      421 agcggtgttg aggggattaa agatggagat attgtacaag gattatcaaa aattaataaa
      481 gatgggactg tagaagttac taccaaggat ggcactagta catatcgtgt gtatgaccgg
      541 gagtttcata acctaatgaa ttcactggtc aagcaggata aagaaaacac tcttggaaag
      601 aaagagggta ctcaaacaga ccttgtatct gatgctgata ttcggaatct ttttggcaga
      661 attgctgcga tctctgaaat ggaaactaat gttggtctgg atgaatatgg gcgagtggca
      721 actagtgcag agagtattaa agaacgcgtg tctttgcaga cttatctggc tacagaggct
      781 gcaaatagcg caattggcct agataaaaac ggtaagctaa ctctgaatga gacagatatt
      841 gtaacgccgt tacgtatcaa tgacggcgtt gtcactatag cgaaagatgt ggaagaacag
      901 aaagaagggc tggcgatcca gaagaaagga ctgtctgagc tttcatcccg cacagatatc
      961 attgatgcag caacggctgc gaatacgaat aaaattgatt cc
//
LOCUS       DAZFFI010000403          997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFFI010000403 DAZFFI010000000
VERSION     DAZFFI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.406"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..157
                     /locus_tag="ACHJ12_005407"
     CDS             <1..157
                     /locus_tag="ACHJ12_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4993007.1"
                     /translation="RETWLVFPQERSGETVSPELTTGAEKRRRRKKKLRTERAEDNPW
                     ITSGGWL"
     gene            154..366
                     /locus_tag="ACHJ12_005408"
     CDS             154..366
                     /locus_tag="ACHJ12_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993008.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            366..>997
                     /locus_tag="ACHJ12_005409"
     CDS             366..>997
                     /locus_tag="ACHJ12_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4993009.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSG"
BASE COUNT          237 a          215 c          337 g          208 t
ORIGIN      
        1 gcgggaaacg tggctcgtct tcccgcagga gcgttctggt gaaacggtat ccccggaact
       61 gacgaccggg gcagaaaaac gccgtcgccg gaagaaaaaa ctgcggacgg agcgtgcgga
      121 agataatcca tggataacat caggaggctg gttgtgagca cagaagaagc cagagaaatg
      181 atacagcggt accgtgaagc ggaaatggcc gtactggagg ggaagtctgt caccttcaac
      241 gggcagcaac tgacgctgga aagcctttct cagatccgcg ccggacgtca ggagtgggaa
      301 cgcaggcttg ccgcgatggt gagccgcagg cggggaaaac caggatttaa actggcgagg
      361 ttttaatggc aattattgat gatgtgatag gcgtgttttc ccccgggtgg aaagcagcca
      421 gactgcgttc aagggcgtta atcatggcct atgaggcggt gaaaccgacc cggacacata
      481 aagcccggcg ggaaaatcgc tctgccgatc agctcagtaa atacggtgcg gtttccctgc
      541 gggagcaggc ccgttttctt gatatcaatc atgacctggt gattggtgtg tttgacaagc
      601 tggaagagcg ggtgattggt gccaggggaa ttattgtgga gcctcagcca ttacgaaaaa
      661 acggggaaat ggcggctgag ctggctgcgg atatccgccg tttgtgggct gaatggtccg
      721 tgagtccgga tgtgacaggg cagtataccc gtcctgtgct tgaacgttta ctgctgcgga
      781 cctggctgcg ggatggtgaa gtgtttgcgc agatggtcag tggtgcggga aacggtctgg
      841 aacggacggc gggagtgcca ttctggcttg aggcgatgga gccggatttt gttcccatgc
      901 gcacagatga atccgccgga ctgaatcagg gggtttttct tgatgagtgg ggaagaccga
      961 aaaaatatct ggtttataaa aattatccgg tcagcgg
//
LOCUS       DAZFFI010000404          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFFI010000404 DAZFFI010000000
VERSION     DAZFFI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.407"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..835
                     /locus_tag="ACHJ12_005410"
     CDS             <1..835
                     /locus_tag="ACHJ12_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4993010.1"
                     /translation="TNHDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQ
                     ADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLT
                     ARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRDSRAPER
                     WPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCNGRVLFERT
                     ALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGISI
                     S"
BASE COUNT          235 a          240 c          304 g          217 t
ORIGIN      
        1 cacaaatcac gacctggctg cgctgtacat gctgaaggtg cagaaaacaa aaaacggcat
       61 tccgtatgtt gccggtattg gtgcggggat tgaggatgct gatggccagc ccctgagcaa
      121 tattctgctg caggcggacc gtatcgcgat gattaacccg gagaacggca acaccacgcc
      181 gctgtttgtg gcgcagggga atcagctgtt catgaacgat gtgttcctga agcgactgtt
      241 tgcagtgagc atcacctcgt ccggcaatcc cccgacgttc tccctgacgc cggagggcag
      301 gctgacggcc cgcaatgcgg acatcagcgg acatatcagt gcgaactcgg gcacgctcaa
      361 taatgtgacc attaaccaga actgccggat acttggaaaa ctgtctgcca accagattga
      421 aggcgatatt gttaaaaccg tggggaaggc tttcccgcgg gactcccgcg caccggagcg
      481 gtggccatcg gggaccatta ccgtcaggat ttatgacgat cagccttttg accggcaaat
      541 tgtgatcccg gcagtggctt tcagcggcgc taaacatgag agagagcata acgatattta
      601 ttcatcatgc cggctgatgg tgaaatgtaa cggcagggtg ctttttgaaa ggacggcgct
      661 ggctgagtct caggtgtatt caggggtgat tgatatgcct gcaggaaggg ggcatatgac
      721 gctggagttt tctgtgtctg cctggctggt gaatggatgg tacccgacag cgagtatcag
      781 cgatctgctg gttgtcgtga tgaagaagtc cacggcgggt atcagtattt cgtgatcatg
      841 aggagccgga ttgccggaag gagacaaaaa ccgtacatta tgcgcgggtg cctttggctg
      901 atggccggag ggagcacctg aaggccggat gtgaaaaggc cccggacaaa cattcatgtt
      961 taacccgagg cctgacgttc ctaccttcaa caagtg
//
LOCUS       DAZFFI010000405          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFFI010000405 DAZFFI010000000
VERSION     DAZFFI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.408"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..776
                     /locus_tag="ACHJ12_005411"
     CDS             <1..776
                     /locus_tag="ACHJ12_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4993011.1"
                     /translation="QYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            888..>996
                     /locus_tag="ACHJ12_005412"
     CDS             888..>996
                     /locus_tag="ACHJ12_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO4993012.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSV"
BASE COUNT          220 a          247 c          291 g          238 t
ORIGIN      
        1 cgcagtatcc gccatatccg gcgctggttc gtcacggaac ggggttgtca tctccggcta
       61 accaggcgtt tgagcagtgg ctggggttgc tggtaaaaaa tgccgttctg gatggtgaaa
      121 cgataaagac cattgagcgt ctttatcacc agtcatgtat cggcgctgtt cgctggggta
      181 atctgccgga taatgccagg gagatcatca ccacgctgat gagccgtcag tattctgact
      241 ggtttggtct ggccgggctg agtgaacaca ttgatgcaga agcctgctgg agctggttgt
      301 cggattaccc ggaacaggcg cagccctgtg acatgctgat ggtcataccc tcccgtctgg
      361 ctacagagct gaatggttca ggtggactgc tgcagggtat ttccacaacg accagccttt
      421 acagccggat atacggtgtg gagtggccgt caggaaataa tgttcgccat gcgcgtgatg
      481 agatgagcag tctggtgctg ctgatcgaca cgccctggta tccgccttca ggcgaactgg
      541 ttggtgagat ctcgaaggtg tttgactgcg aggtccgaca ctggtacagc gagccggtac
      601 acggtataca gggttacaac tgttacgatg gcggagagca tacggacagt gatctgcagg
      661 ccgaatggcc gggcagggaa acacttccac agccccgact gtacctggtg gaggagtgcg
      721 ctgaagaaca gacgaaagcc gcacctttac cggttcctct ggtctcagcc cagtaattaa
      781 cctccgggta tcatgtccgt gatgcccgtt ttatcaacat ttcaacccga aggggaacac
      841 ctgttcccct gtccgggcca tgtgttccct gtttttacgg agaaaacatg gccaccatga
      901 ctgaccatca gaaggagttc atttcgttgt ttaaccggac agcctgttat cacaaacgtc
      961 ataaggtgtt tgaagacttt gtcagctgta gtgtga
//
LOCUS       DAZFFI010000406          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFFI010000406 DAZFFI010000000
VERSION     DAZFFI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.409"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..742
                     /locus_tag="ACHJ12_005413"
     CDS             <1..742
                     /locus_tag="ACHJ12_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4993013.1"
                     /translation="GINLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHELEE
                     MDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNI
                     MDYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNR
                     LIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKI
                     WYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            744..>994
                     /locus_tag="ACHJ12_005414"
     CDS             744..>994
                     /locus_tag="ACHJ12_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO4993014.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDR"
BASE COUNT          322 a          162 c          193 g          317 t
ORIGIN      
        1 aggtataaac ttagattcag cattctcatt atctgaagaa gaacgctcat attttgaaga
       61 aaaagctaaa agtttaaacg cgcttgcaca gttaggtatt agtgttgaga taatatctca
      121 tgaacttgaa gaaatggatt ctatggtaac cagaggacta aactctcttc ctacttctgt
      181 aaaagaacac cctggttttt cattggcgtt aaatgctcac agatcgctta ctcaacaaat
      241 acgtttctta tcacctttga aaatatcagg ttatcaatcc aggcagagaa taactggaaa
      301 aaatatcatg gattatgtcc tgaagttctt tggggagcgt ttcgaacggc aacgaataac
      361 tattgaattt agtgaagagt ttaagcaaat cgcaataaca gatataccat caaggatcta
      421 tcctgttttt actaatatta tcaacaatgc aatgtattgg gtcagtctgt caaataatag
      481 gctcataaag attggttttg tgaattcttt ggttatcata gcaaattctg gtccggcaat
      541 tgataccgat gatatcccgc gactatttga actattttat agcaaaagag caaatggaca
      601 tggggtaggt ctgtatctat gtcgagaaaa ccttgctgtt gcacatcata aaatatggta
      661 ttcagaacct gatgaaggcg ataactattt aataaaagat ggcgctaatt ttgtgatcca
      721 gttcaatgga gtggagttct aatatgacag tggcaaatta taattctctt gtccagaaaa
      781 ctttctgcga aaatgcaatt cgttccgttg tcatgattga tgacgatttt ctgacgtatt
      841 ctgaatcaat cagggcgttg aataacgaag ttgatttaga ctacaacaaa attgactcat
      901 ctaaacgagc cgctactctt gagagctttt ttcaatctaa aaatatgatt tgtgatgttg
      961 acaatggttc tgttaatttc gatgtggatc ggat
//
LOCUS       DAZFFI010000407          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFFI010000407 DAZFFI010000000
VERSION     DAZFFI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.410"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(558..743)
                     /locus_tag="ACHJ12_005415"
     CDS             complement(558..743)
                     /locus_tag="ACHJ12_005415"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993015.1"
                     /translation="MIANPFVGLLGLIMGIIGLRIGCEMIILLFNIYKQVKKIADAVP
                     DKINEQAEDSLLSQNKH"
BASE COUNT          343 a          231 c          175 g          237 t
ORIGIN      
        1 tacacgcgta agcgaccaaa tttcaaatgg tcttatacag cgcgcacagt cgcaggaagt
       61 gctggcaaac aaatgaaaag aataaaagaa gtcaataccg ccattacgca gtaaaaacat
      121 tccggcgtca ccggatagat aaggatcaca caaccactgc tgtgtattgc ccgtaataaa
      181 gggcgcggtt aaaagaaacc ttcatcatca tgactgacga tatctgctct caaaggtggg
      241 cagatttacc accaccagaa acatcctggc tgcacatccc ctttcaggtt acgggggtgt
      301 tgatcacccg aaggcgttcc tctcagatca acggaacatt ggtgcccacc gaagcgggat
      361 gtggtcaccc gaaggcgctc ctctcagacc acttatggag cattggtggt cgccaaagcg
      421 acagataatg ctttctgagc atatcagagc gtataaaagg cgtcaatcat cactcagaac
      481 ataaccagga agaacagaaa acagtctgca gaaaaaataa cagggacagt attaacagtc
      541 cctgttatta ccattcatca atgtttattc tgagataata aactatcctc agcctgttcg
      601 tttattttat ccggtaccgc atcagcaatc tttttaacct gcttataaat attaaaaaga
      661 agaattatca tttcacaccc aatcctcaga ccaataatac ccataataag accaagcagt
      721 ccgacaaacg gattggctat catacctata acaccaccgg ccactgtaat aaaaagaaaa
      781 aaaccagtat attaccttga taatcttcgg agtaatcatc tcatcaaaat caatccactg
      841 actgaacatt ttattcattt tcacctctcc agcaatcggt tgttaacagc aatgtcaaaa
      901 taaaaacact gctaacgcgt aaaatacaaa cagataaaga atgtgtcaat atgttacatg
      961 tactaaaaca tcagaattat gtttag
//
LOCUS       DAZFFI010000408          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFFI010000408 DAZFFI010000000
VERSION     DAZFFI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.411"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(798..>986)
                     /locus_tag="ACHJ12_005416"
     CDS             complement(798..>986)
                     /locus_tag="ACHJ12_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFO4993016.1"
                     /translation="DLIMNLVMGTMFLVLPAVWLGALSWAGVHAGVIASGIQRGTETV
                     SGAAQTAGTIARSGVKSK"
BASE COUNT          298 a          233 c          181 g          274 t
ORIGIN      
        1 tgatcaccat caaaaaaaca aatgtaatat gctggcgaat ttatattact gagtttttca
       61 tatacgtcat cgacggttca tacgaatgat cgcagtctga tgatggtcgt gttttatttt
      121 gttaaacaag ggttatcacc agacaagtac cggaggctgg cactgaagta ccaactcgtg
      181 tgattgcgtt gctcgtgaag gactgacact gaaatctcat gtctagtgat gaaattctga
      241 ccaagataaa gcgcgcaata tgagacgtct tgtgatagtt gcccagtctg gcaactatcc
      301 cccttaatgg gacgcttact cataatctaa cgataagaaa cattctcatc tggatcattt
      361 caacagaatc cccgagcccc agggtaatgt ggtatcgcag cctctcatca aatggaagac
      421 atgtgattgc acatttatca tacaagacgt atgataacac tgtgttattt atcctgcgcc
      481 gagggtatag atatgaataa cgaatctgaa aaagcaatgt ggcgttgtaa gccagtggat
      541 aatccttgga cattcagttc ctttgcaaaa aaggtcattt gttcagtatt acccgatact
      601 cagccctcgt tagacgcatt tgtaaattca acaaactctg aggaacactt aaacttttca
      661 tcagactcaa ccttcccttc atgcccagac atcaataaca tcaataacat caataacatc
      721 aataattatt attgattcta agtttttgtc gccgaaacca ataatagtaa atggcggtaa
      781 aaccgccatt tacgttgtta ttttgatttc acaccacttc tcgcaatagt tcctgctgtc
      841 tgcgctgctc ccgaaacagt ctctgtacca cgttgaattc cagaagcaat tacccctgca
      901 tgtaccccag cccaggacaa cgctcccagc cacactgccg gcaagacgag aaacatcgtc
      961 cccatcacca gattcattat cagatc
//
LOCUS       DAZFFI010000409          979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFFI010000409 DAZFFI010000000
VERSION     DAZFFI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.412"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..341
                     /locus_tag="ACHJ12_005417"
     CDS             <1..341
                     /locus_tag="ACHJ12_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO4993017.1"
                     /translation="ELLPPGEQSMLEIWQGKGSGGHSRHMTAARDTLRRPLTMPEQQA
                     DAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGTSPVPGYSTVFPFYSRTQY
                     AMPGERTEAL"
     gene            425..>979
                     /locus_tag="ACHJ12_005418"
     CDS             425..>979
                     /locus_tag="ACHJ12_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO4993018.1"
                     /translation="MKERVAPQVRKALSRPGKMTRQDETKVYHACPSVIDFLPWVEYL
                     DEEQCLLLDDGVSVGAVYEVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVV
                     QFFCQDENDVDTYVDQLRGYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVT
                     GQPWRGQQRRTRMVIYRWIGKNNHD"
BASE COUNT          227 a          228 c          326 g          198 t
ORIGIN      
        1 aggagcttct gcctccgggt gaacagagta tgctggagat ctggcagggg aaaggaagcg
       61 gaggacattc gcggcacatg acagcggcca gggacaccct gcggcgtccg ctgacaatgc
      121 cggagcagca ggcagatgcg cacggagcca taagctacag ccggacacag gaaagtgaga
      181 tcagtcagca gtttccgcgg ctgcccaatc ctgacatggt gatgtatgtt tttccgcatc
      241 tggcttccgg tacgtcaccg gttccggggt acagcacagt atttcctttt tacagccgga
      301 cgcagtatgc catgccgggt gaacgaacgg aggcgttgtg atgcgatttt ccctgtttac
      361 agacacaaaa aaacgggttt cttccccggt gtcgcagcat gacggagaag cggtatctcc
      421 ggatatgaaa gaaagagtgg cccctcaggt acgaaaggca ctgtcaaggc cggggaagat
      481 gacccgacag gatgagacga aggtttacca tgcctgtccg tcggtgattg attttctgcc
      541 atgggtggaa tatctggacg aagagcagtg cctgctgctg gatgatgggg tgtcggttgg
      601 tgccgtgtat gaggtgagtc cggccgctac ggaagggcgg actgcagagc gtctggagca
      661 aatccgggat acggtggagg atgcgctgca ggacagtttt gatgaatatg actcacatcc
      721 ctgggtggtg cagttcttct gtcaggatga aaacgacgtg gacacgtatg ttgaccagct
      781 cagggggtac gtgaaacctc atgcagaagg ctcctcattt acggaggcct ggctgaggga
      841 aatggagcgc catctgaagg ggattgcccg accggaaggt ctgttcaggg atacgctggt
      901 cacaggtcag ccatggcgtg gtcagcagcg gcgcacgagg atggtgattt accggtggat
      961 cgggaaaaac aaccacgat
//
LOCUS       DAZFFI010000410          977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFFI010000410 DAZFFI010000000
VERSION     DAZFFI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.413"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..126)
                     /locus_tag="ACHJ12_005419"
     CDS             complement(<1..126)
                     /locus_tag="ACHJ12_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO4993019.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDD"
     gene            247..525
                     /locus_tag="ACHJ12_005420"
     CDS             247..525
                     /locus_tag="ACHJ12_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993020.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(517..807)
                     /locus_tag="ACHJ12_005421"
     CDS             complement(517..807)
                     /locus_tag="ACHJ12_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993021.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
BASE COUNT          240 a          206 c          236 g          295 t
ORIGIN      
        1 atcatctgaa gcataaacat aatgccgggt atgtcgttcc aggctggtct ttggtgtgca
       61 tcctgtaaga acgattgttg ctgttatcag aggtatcaga agcagcctgg caacggcaga
      121 tctcataaac atctccttgc gtttttaatc ttgatactca gggagttata tttccgaaga
      181 gtatctctta tcatacgtcg gtttacccca atgtgatttg atggtcaatg agtcaggaat
      241 aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg ttctttctgc ttcggttgcc
      301 agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga agacaaaaaa tggcattgtt
      361 gcggatgtta atgccagaca gaaaatgttc agtaacagac tgaagataca acttcagatc
      421 gatgagttga gggcatctct ggctggcacc agagatgccc aaaaaagaga ggatttgcgt
      481 aaaaaactgg agtctctgga gcggcaaaag aaatccttac gctgatgggg gcgtattttc
      541 cccggtttct tccataacct ttttaccgtt cagatattta cgggcgaggc gaatgcgtct
      601 tgcctggtat accgccgagt aagcctgggt gggattcaca ccgttactga tttcactgac
      661 cacatggcgc tgatcagcca gtgtcagcgt gtaaacctgt ttctggatga ggtcacgggc
      721 tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga agtcgtttgt ctgttttgtt
      781 cagtttatgg cggggtgatt tgctcacatc gttctccgtt tttcatgatc caacagtttt
      841 atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc tgcctcccca
      901 tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca atggtgagta
      961 gtaggttttt ctgtaca
//
LOCUS       DAZFFI010000411          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFFI010000411 DAZFFI010000000
VERSION     DAZFFI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.414"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..915
                     /locus_tag="ACHJ12_005422"
     CDS             13..915
                     /locus_tag="ACHJ12_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4993022.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
BASE COUNT          307 a          208 c          167 g          292 t
ORIGIN      
        1 ccggagtagt gtatggccaa tctctacgac ttgaaaaagt tcgacttaaa ccttcttgtc
       61 atatttgagt gtatttacca gcatttaagc attagcaaag ctgctgaatc actatatatc
      121 accccatcag ccgtgagcca gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta
      181 ttcattcgct caggaaaagg tatcgcgccg acgacaacag ggttgaatct gcatcaccat
      241 cttgagaaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
      301 aagaaaaact ttattattta cggccctcag ctcatttctt gctctaataa tagtatgctg
      361 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
      421 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
      481 ccagtgatca gccgttccgt catttgcatg ccattgcata cgattcgcaa tactctaatt
      541 tgtagtaata ggcatccacg aattactgac aatagcactt atgaacaaat tatggcagaa
      601 gagttcaccc aattaatatc taaatcagca ggggttgatg atattcaaat ggaaatagac
      661 gagagattta tgaatcgcaa aatctcattt cgtggttcct ctttgctgac aatcattaat
      721 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ttcctaccgt
      781 gactttctta atttaaaaga gataaaacta gaacatccgt taccttcaat aaagctttac
      841 atttcctata ataaatcttc tttaaataat ttggtgttct cccgatttat tgaccgtcta
      901 aatgagagtt tttgatatat aactacagac agctactata actttcatct atttattcac
      961 agcgcgaatg aaaa
//
LOCUS       DAZFFI010000412          967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFFI010000412 DAZFFI010000000
VERSION     DAZFFI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.415"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..527)
                     /locus_tag="ACHJ12_005423"
     CDS             complement(<1..527)
                     /locus_tag="ACHJ12_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO4993023.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLG"
     gene            724..>967
                     /locus_tag="ACHJ12_005424"
     CDS             724..>967
                     /locus_tag="ACHJ12_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993024.1"
                     /translation="MNTEKQLPSGIESEVKYTLQFWLLSNGKVMTEKDGLCNPFPLNC
                     IESAHPFTPFQQNQWIELDTVGSDGPVKATFLVHSVL"
BASE COUNT          237 a          266 c          248 g          216 t
ORIGIN      
        1 ccgagcacac tttccagttg ctgcagaacg gtatcaagcg cggatgaaag agaggcttcc
       61 gtgcgctccc ggagcgtcct gttctgcgca agcgcaaaca gacgccgctc tgcagcatcc
      121 agcacatccc gtgacgtggc atttggtgcc gccacatcag caatcagggt gtttccggtg
      181 gcctgtaact gacgcagaac gcttttctcc accacaatgc ctgcataggc catgatgttg
      241 gccgcagaag gtgtgttctt gctgagctcg gccagataag caaaaccgcc cagtacgtcc
      301 agcctgccct gatgttccag gtgctctgac agtgtaatca ggtccagtgg ctggccattc
      361 accgccagtt cagacatggc acggaaaatg gcccggtgct gagccacata aaaatcgtca
      421 ggactcagca tcagcaccac ttcatcccag cgttcattct ccagcatcag cccacccaga
      481 acggcctgct ctgcttcgta tgcgcagaga acagacattt cagccatggc gtaccctccc
      541 tctctgactg ccggaaacct ggctacgtga aaaaaggaaa aagggtgccg gacgtacagt
      601 tgtcgggaat aaaatcccgg taactgtctg tacgttttca gaccggtgag tttgccgacc
      661 ccggtcagaa ggtacggaca gcattcgttc attcataaca agttgtttta aaggaaaaag
      721 tttatgaata cagaaaaaca actgccgtcc ggcattgaat ctgaggtgaa atacaccctg
      781 cagttctggc tgctgagtaa cggaaaggtc atgacggaaa aagacgggct ctgtaacccc
      841 tttcccctga actgtattga atcggctcat ccttttactc cgtttcagca gaatcagtgg
      901 attgaactgg acactgtcgg gtcggatgga ccggtgaaag cgacgtttct tgtgcacagc
      961 gttctgt
//
LOCUS       DAZFFI010000413          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFFI010000413 DAZFFI010000000
VERSION     DAZFFI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.416"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..462
                     /locus_tag="ACHJ12_005425"
     CDS             <1..462
                     /locus_tag="ACHJ12_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4993025.1"
                     /translation="TDINNRTDGLLMTERLARIAGDAETLATAEAYSDAGDSLTLKQA
                     TAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYVAENSF
                     SYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            complement(747..>959)
                     /locus_tag="ACHJ12_005426"
     CDS             complement(747..>959)
                     /locus_tag="ACHJ12_005426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993026.1"
                     /translation="MEDFRGALHKVRLENKAQVSESHRGQTALTAIRADTELQLLVDF
                     RTTESLQTGMCSFRGYDATTNAFSDY"
BASE COUNT          272 a          260 c          212 g          215 t
ORIGIN      
        1 accgacatca ataaccgtac tgacggatta ctgatgaccg aacgcctggc acgcatcgca
       61 ggtgatgcag aaactctggc aacagcagag gcatacagtg acgccgggga ttcactgaca
      121 ctgaaacagg caacagccta cagcgatatc agtacccgcc gggcactgtc agaggccaat
      181 cgttataccg acaacaaatt cagaaacctg agcaataaaa ttgaacgggc agaaaaaaga
      241 cttaatgcag gcatcgccgg agtgacagca atatcctcca tcccctatgt ggctgaaaat
      301 tctttttcat acgggatcgg cgtgggtaac tatcagaacg ctaatgctgt ggccgcaggc
      361 attcagtata aaacatcacg taataccaat atacgcctca acatctcctg ggactcatct
      421 cataacaccg cgattggtgc aggggttgcc ggcggctggt aaataatctt gcgtacatac
      481 ggcattatgg tattccgtaa tcacattaat tcagtacgca acacagcccc atccggaaca
      541 gaccactgga ctgagcggga taatcaagga aaaagggcgc gcagtgtggt cagccttcac
      601 tacaatccgg cggcagattt cctgccgcca tgttgcccgt aaacatttaa gttcgcaggc
      661 taacattatt gccacaagcc ccgtatttcc ctggtgaatc ctcaaagcga gtccctcacc
      721 atccagacaa aacacagtat cgtcgattaa taatcactaa aagcgttcgt cgtagcatca
      781 taacccctga atgaacacat ccctgtttgc aaggattctg ttgttctgaa atcgacgagt
      841 agctgcaact ctgtatccgc ccttattgca gtcagtgcag tctggccacg atgcgattcg
      901 ctgacctgag ccttattctc caggcggact ttatgcaatg ctcccctgaa atcttccat
//
LOCUS       DAZFFI010000414          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFFI010000414 DAZFFI010000000
VERSION     DAZFFI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.417"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..328
                     /locus_tag="ACHJ12_005427"
     CDS             <1..328
                     /locus_tag="ACHJ12_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993027.1"
                     /translation="NNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYLLDK
                     ENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMK
                     ITGTPE"
     gene            340..789
                     /locus_tag="ACHJ12_005428"
     CDS             340..789
                     /locus_tag="ACHJ12_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFO4993028.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          321 a          117 c          236 g          285 t
ORIGIN      
        1 gaataatatg gcgatatatt ggaatagtag agcatcttta ttttgttcaa ctgaattaaa
       61 ctcgaaatca caatcgcctg cattaggttt gggacatgag tttgctcatg cgcagtatta
      121 ccttcttgat aaggaaaatt tcatggctct tttaagccga acagataaga aatatgagaa
      181 taaagaagag gcaagggtta ttacaataat tgaatcgcgt gcggcgaaaa ctttaggtga
      241 gtgtaccaga ggagctcata gtggactgcc tttttatcgg gtggatggac cgttgcagac
      301 gatgaaaata acgggtacgc cagaatagtg aggtttttta tgatgaaata tttgatggtt
      361 ttattgtctc tattttctgg ttcggtactg gggatggggc gagtgaatga attgtgtgga
      421 atagatagcg tgaaaacgat agagataatt aatcttccat cgtatgttac cacgttagtt
      481 ccgcttagca aggaaggtct taatgaaata tacagatata aagtggtggt gaatgaaatt
      541 agcgatcttt atgctggtaa gataattgat ttattgcaaa tgaagtattt cagaaaagaa
      601 aaatataaca atataagatg gggggttagt atcatttcga aaggtaataa taaatgtgaa
      661 atttattttg atgcttttgg tgaatgtgga agtgtgaatg gcattaacgt gtgttttgaa
      721 aaaaatgaaa tgataggttg gattaaaaaa gaaatccctt tgctgtcgca aaaaataggt
      781 gggctttaaa tggtagggca atattcaaag acagtatagg taactatttg tatggccatg
      841 taaggtaaaa gaaaaggaat aaaccatgag cggaaaaccg gcggcgcggc agggcgacat
      901 gacccgcagg gaaagatcat cagaagttca ctttttgtac taaataattc gcattttat
//
LOCUS       DAZFFI010000415          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFFI010000415 DAZFFI010000000
VERSION     DAZFFI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.418"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(181..852)
                     /gene="queE"
                     /locus_tag="ACHJ12_005429"
     CDS             complement(181..852)
                     /gene="queE"
                     /locus_tag="ACHJ12_005429"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4993029.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          255 a          237 c          208 g          256 t
ORIGIN      
        1 accaaacttt accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag
       61 agaatgctaa tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca
      121 atataaatga gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa
      181 tcaggcaata tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca
      241 ggtttcaatg cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac
      301 tcgcggttta tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcacgtac
      361 gcgccccact ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata
      421 gccgccgcgc atgttcagct ttggtgatac ggtaacccag gtattcggtg tgcagcgtac
      481 ttcatgagtt ccactggttt cgatctggca gctaaaaccg ttcttttcga gcaggtcagt
      541 cagtggcagc aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt
      601 atatccctgc cgaccaatga cagccagcaa atcttcactg ctcgccgccc cccacttatc
      661 actctctttg gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc
      721 ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa
      781 aatggcgggg acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat
      841 cgggtactgc atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgccg
      901 caacaatcat gtcttattaa cattctgtta catgcagatt taaagaaaaa tgcgaa
//
LOCUS       DAZFFI010000416          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFFI010000416 DAZFFI010000000
VERSION     DAZFFI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.419"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..322
                     /locus_tag="ACHJ12_005430"
     CDS             143..322
                     /locus_tag="ACHJ12_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4993030.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            363..608
                     /locus_tag="ACHJ12_005431"
     CDS             363..608
                     /locus_tag="ACHJ12_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4993031.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            685..900
                     /locus_tag="ACHJ12_005432"
     CDS             685..900
                     /locus_tag="ACHJ12_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4993032.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          245 a          208 c          281 g          209 t
ORIGIN      
        1 gccgccagca atgaaggaaa gggatgaact gtgtccacta catgaatgct ggcggctggt
       61 aaaacgacca tgatgacacc gaagtctgcg gtgtttttct acaaaaataa gtaaatcatg
      121 tcaactatca cggtgagaaa ccatgacatt tctgaaccag ttaatgctgt acttctgtac
      181 ggcggtctgt gtgctgtatc ttctttcggg tgggtacagg gcagtgcgcg atttctggcg
      241 caggcagatt gataaaaggg ccgcagagaa aatcagcgcc agtcagtcag ccggaagcaa
      301 acccgaagag ccgctcattt agctgcaact ttcttaatca cacctttcga cgagaaaatc
      361 ccatgtcaga aattacatcc ctggtcactg cagaggcagt gaaggaagtc ctgcgctctg
      421 aagaagtcct gagcgcactg aaacagaaac tccgccagaa ccttgaggcg cgtcttgatg
      481 cagaagtgga tgccattctg gatgaactgc tgggcgcacc ggcagttccg gagccggaag
      541 gtatcgcggg tgaggggagt gcttcagatg gcggtgaccc cacacctgac agcgacatga
      601 tgatgtaagc atgcgcaagg gaccatcggt gtgtgccggt ggtctttata ttgttgtgag
      661 cttccggatt gcgggagacg gggtatgtac cagatggaaa aaatcacaac aggtgtgtca
      721 tacaccacgt cagcggtggg aacgggctac tggttcctgc agttgctgga cagggtttcc
      781 ccgtctcagt gggcggcaat aggtgtgctg gggagtctgc tgtttggtct gctgacatat
      841 ctgacgaacc tgtatttcaa aatcagagag gaccggcgta aggcggcgcg gggagagtag
      901 acgatgaata aacaatacga actggttgtt aaagggataa ata
//
LOCUS       DAZFFI010000417          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFFI010000417 DAZFFI010000000
VERSION     DAZFFI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.420"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..811)
                     /locus_tag="ACHJ12_005433"
     CDS             complement(<1..811)
                     /locus_tag="ACHJ12_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO4993033.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTMHSLAC"
BASE COUNT          235 a          248 c          240 g          200 t
ORIGIN      
        1 cacaggccag actgtgcatg gtgaccagcg cagaatcgct gcaacgggca gacacttttt
       61 ccagacgatg caccggctga ccggctgcgt gcccctgttt ataaatattc aggtcgtaaa
      121 tctgtgttga aagaagggtg accgcacctt caggcagcac tttgctgtag cgaccggcgt
      181 tgataatgcc tgtcagctct gccagtgtct gtctggccag cgccgtataa tccatgcagt
      241 taaccagggt gcggactttc ggcaggggaa tacccagttc acggtagggc ataaggtttt
      301 ccatgacatt gaccgtgccg cggatgaact cacgcacatc cggcagaacc ggattcacca
      361 tccccaccac agactccgtg gcggacagca caatcatttc cagcatgaca gaacgtgcgc
      421 cctgtgaatc caccacaatg acgtcatagg tctgaaaaag cggatgctga agcacattac
      481 gcagacgaag gcgaccatcc ggcgcatgaa gcatggccgt tttcagttgc tcatgggggt
      541 cgttggagac gatgaggtca agccccctga cagccgtacg ggaaatgatg ttgtcaggat
      601 gactgaggtc agccgtgcgc atcagtaatt caaaaagacc ggccggcgct tcatacgtca
      661 gcggaaagat attgcttgcc gtgggctgtg catggtctcc gtctatcagc agagtacgga
      721 ggccggcatc tgccagaaaa ccggcaagat tagcagactg ggttgatttt ccttcgccac
      781 ctttagtgga tataaccggt aaaattttca tatagcacac tctttaaagt atgcaaccaa
      841 tcacttacac tacggaaacc agccagaata ttcacttcac tacctgaata ctcgcaacac
      901 ggggattgaa aatccccgtg tcc
//
LOCUS       DAZFFI010000418          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFFI010000418 DAZFFI010000000
VERSION     DAZFFI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.421"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..174)
                     /gene="hha"
                     /locus_tag="ACHJ12_005434"
     CDS             complement(<1..174)
                     /gene="hha"
                     /locus_tag="ACHJ12_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4993034.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNK"
     gene            complement(200..574)
                     /gene="tomB"
                     /locus_tag="ACHJ12_005435"
     CDS             complement(200..574)
                     /gene="tomB"
                     /locus_tag="ACHJ12_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4993035.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          269 a          193 c          181 g          280 t
ORIGIN      
        1 tttattcatg gtcaattcgg cgaggcggtg atctgcggct gagtaaaata ccgccagttc
       61 attatctgat aattcgtatt tatttttctc gataacacgc tccagcgtgt caattgtctg
      121 gcaacgacgt aaacgcatta aataatcggt tttcgttaaa ggtttttcgg acatacttct
      181 acccatggtt gtaataattc taacaagata aactcgcagg attctctttc gtcgcattga
      241 caaaacaacg gaatagtcga ttacctgact tccgccattt ctgaagatcc tgcatattaa
      301 taccataact actgaacaac ataaaggtgt catccagata ttcgtcgatc tgctcaatga
      361 gcttattgtc ttcattatac ttaattttgt aattaagtgc gaaggtcgca atatgctcaa
      421 tcagttcatt caactggagg ttgatcgccg aggttgggtc gttaacccag ccatgattgc
      481 tttcttcaag gtttgcaagg cagtcatgat acagggtttc acagagaaac ttaagctgtg
      541 cgatatcatg tcttttgggt gagtattcat ccataacgcg tccccttctt agcggttgaa
      601 ctaacggaca cctttcggga tggaaaaaac ttactgacct ggacttgccc tctcgtttgt
      661 tagcttaact ataagccact ctttgcaggt tttcatcgca ttattacgaa aaattacaat
      721 taggaaggaa tcgggacagg actcactttt tcatactagc actaacccgc agcaggtaaa
      781 agcagtctgc tcgaactgtt cataacattt caggattatc gccagatgaa attggtgaca
      841 ctaataccag gattgctctg aatatgacgt aataaccgag gaatgaataa agaattaccg
      901 caaataatta agaatagcct cta
//
LOCUS       DAZFFI010000419          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFFI010000419 DAZFFI010000000
VERSION     DAZFFI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.422"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..921
                     /locus_tag="ACHJ12_005436"
                     /pseudo
     CDS             1..921
                     /locus_tag="ACHJ12_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          226 a          208 c          289 g          198 t
ORIGIN      
        1 cgcagacggt ggaacaactg aagatgaccg ctgaaattgt ctgttcggtg ttccgtgtcc
       61 cggcctacaa gattggcgtg ggacaaccgc cttccagtga caacgtggag gcgctggagc
      121 agcagtatta ttctcagtgc ctgcagacgc tgattgagtc cattgaactg ttactggatg
      181 aggcgctgga aacgggggaa aacgagagta cggaatttga tgtcaccacg ctgctgagaa
      241 tggacagtga gcggcgcatg aaaacgctgg gggatgcggt gaaaaatacg cttctcacgc
      301 ccaatgaggc ccgtaaacgg gagaacctgc cgcccctggc cggcggtgat gcactgtatc
      361 ttcagcagca gaactacagt ctggaagcgt tgtcccgccg tgatgcccgt gaagatccgt
      421 tcgcgtcgtc cggtaaaaca gcttccgtgc ctcaggcggt cgttgcctct gacggtaata
      481 aggcaatcac tgaaacagag catgatgcgg tgaaggcgat gttcaggggg attctgaaaa
      541 aatgaatgaa cgtgaactgt ccattatccg tgcgctgggt gaagaatttt ccgcagtgct
      601 ggcggattta cagcgcacat ttgaggggaa aatagccgcg caggcacaaa cgtttgaaga
      661 aaaactggct tccctgtctg tggtattaca gaagtgcgtg acgggcgatg atgtgcgtcc
      721 gatgcttgag cagatggtga aggaggcggt aagccatatc cctgttccgc gcgacggtcg
      781 tgactatgat cccgatgttc tgcagaaggc ggtgaatgat gctgtcggga aaataccggt
      841 accggcgaac ggtaaaagta ttactcccga tagtgtgcgt ccgatgattg agcagatggt
      901 gaaggaggct gtaagccata t
//
LOCUS       DAZFFI010000420          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFFI010000420 DAZFFI010000000
VERSION     DAZFFI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.423"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>911
                     /locus_tag="ACHJ12_005437"
     CDS             <1..>911
                     /locus_tag="ACHJ12_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4993036.1"
                     /translation="SPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNFFTLLS
                     GNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQCGVINPQNANTAYFGDTDGRV
                     GAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRDMILNE
                     LHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVV
                     VLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEE
                     KKCYCLGALQVPFNQMKNPTDPEELAN"
BASE COUNT          296 a          141 c          182 g          292 t
ORIGIN      
        1 cgtctcccat taccgttact gttgagtgta agggcggtta ttattttatt aattcctctg
       61 gagataaatc agatataatt tataaagttg atgggctttc tatcatagcg agaaatttct
      121 ttactcttct tagtggtaat ttcaaacctg actggcgttg ggatgtctct aaagagacgt
      181 ttactaagga aaagtttgat agctatgtta aacctgtgtt ttcaaaaata gacttctata
      241 aacagtgtgg tgtgattaac ccacaaaatg ccaacacggc ttattttggc gatacagatg
      301 gaagagtagg cgctgtactt tatgcattgc tcgtttcagg gcatataggc atcagggaaa
      361 agggttggag tttattatgt gaattattaa aacacgaaga gatggcttca tctgcataca
      421 aacataaaaa taataaagtg ctctacgatt tattaaatac gagagacatg atcttgaatg
      481 agttgcatca acatgttttt ttaaaggacg atgcgataac cccttgtatc ttccttgggg
      541 atcatacagg cgatagattt agtactattt ttggtgataa atatatcctt actttgctaa
      601 attctatgcg aaatatggag ggtaataaag atagccgtat taataaaaat gtcgtcgttc
      661 tggctggaaa tcatgaaatc aattttaatg gtaactatac tgcacgacta gcaaatcata
      721 aactctctgc cggtgatacg tataacttaa ttaaaacgct cgatgtttgt aattatgact
      781 cagaaaggca agttttaacc agtcatcacg gaataataag agatgaagaa aaaaaatgtt
      841 attgtctcgg tgctttgcag gttccattta atcagatgaa aaatcctact gatcctgagg
      901 aattagccaa t
//
LOCUS       DAZFFI010000421          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFFI010000421 DAZFFI010000000
VERSION     DAZFFI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.424"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..139)
                     /locus_tag="ACHJ12_005438"
     CDS             complement(<1..139)
                     /locus_tag="ACHJ12_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993037.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KT"
     gene            complement(132..>907)
                     /locus_tag="ACHJ12_005439"
     CDS             complement(132..>907)
                     /locus_tag="ACHJ12_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4993038.1"
                     /translation="GDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGL
                     QEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSWAASILQEISKV
                     LMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDT
                     PTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQN
                     DGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          181 a          303 c          200 g          223 t
ORIGIN      
        1 aggtcttcag ttgcgcattc agtcctgccg gacgacgttg ctgataaccg tcgccaaact
       61 tcaccgtcac caccgacggt ttctccgtca cctgcatccc ttcccgggga caccagtgaa
      121 gggtcttaat ctcatccact cagcattcct ccgtcacgac gcatggataa catcaccgcc
      181 tgtacccgct ggtcaatcag ttgcacaagg gtgcccgccg cttccggccc tatctgcccg
      241 ttagcgccgt cattctgaat ggcaatgtgg tacaccgggg aatacaccag acccgcactg
      301 ccgttcatac tgcccaccgc acgcacgccg agtgaaccat ccgctgcccg ggtcaggggc
      361 atgatggctt caggtccggc ttcccccatc agccctgccc cttttgcaaa ggcaaagtac
      421 gtgggcgtgt ccacaatact gttactgtat gcactcaggt ttgccgaggt ataaacgccg
      481 ccttttgcat ttgccaccgc gccacccagc cagttgccta tactgccgaa aaatcctcct
      541 gcaccggaca tactgtttgc cgccatctta atgccgttga caatggccgc attcataaga
      601 acttttgata tttcctgcag gattgatgca gcccagctgc gccattccac tttatttccg
      661 ttcagcatct ccgtgatgtt attcaccatc cctgagatac cgtctgtcgc aagctgtgct
      721 gcctgtgaag cgtaatctga tgcgctgtcc acccagttac ttagcccttc ctgcagccct
      781 ttctgccagt cagcacgctg cgcatccgat tcggcataaa aagcctcctg gtctttaagg
      841 cgttcactca gatactgcgc attctgcgcc agcgcctgtc tgtaaaaatc ctcactgata
      901 tcccctg
//
LOCUS       DAZFFI010000422          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFFI010000422 DAZFFI010000000
VERSION     DAZFFI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.425"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..522
                     /locus_tag="ACHJ12_005440"
     CDS             <1..522
                     /locus_tag="ACHJ12_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993039.1"
                     /translation="AGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVDVF
                     RGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKGQN
                     ITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGEFA
                     AVAEITVTDGAAG"
     gene            538..>903
                     /locus_tag="ACHJ12_005441"
     CDS             538..>903
                     /locus_tag="ACHJ12_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4993040.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPT"
BASE COUNT          218 a          231 c          272 g          182 t
ORIGIN      
        1 gccggtgaca ccagttttac gctggcctgg aagcccggcg agaaagggca gcgtgatttg
       61 attgcctggt ttgacagcag cgagacccgg gcctacaaaa tccgcttccc gaacggcacg
      121 gtggatgtgt tccggggctg ggtgagcgcc attggtaaat ccgtgaccgc caaagaagtg
      181 atcacccgca ctgtgaaaat caccaatatt ggtcgtccgt cgctggctga ggatcgggga
      241 gaaatcacac cggtcaccgg tattaccgtg acgccactaa cggggaatgt ggcaaaaggt
      301 cagaatatca ccctgaccgt ggccgttcag ccggaaggcg caacggataa aacgttccgc
      361 gcaacctcag cgaatcagaa tttcgccacc attaccgtga aggggaacac gatcaccgtg
      421 aaaggtgttg cggcaggtaa agcgcagatc cctgttgtca ccggcaatgg tgagtttgcg
      481 gcggtggcgg agatcaccgt cacggatggc gctgcgggct gaggggggag ataaagcatg
      541 tttctgaaaa cagaacaatt tgaatataac ggggtgtccg tcacgctgtc ggagctgtct
      601 gcgctgcagc ggtttgatta tatgaagttt gtttcagacg cagcacaaca ggagacaacg
      661 gagcatgatg ccgtgcacat taaccagcga tatctggaaa cggcatccct gcttgtggcg
      721 atgtcgctat ggcattccca ttccctcaaa ggcactctgg cctctccgga gacagagatg
      781 cagcagatcc gccgtgaagt gatgctggga tggcctgctg atgcactgaa tcaggcaacg
      841 aaccgggtgc tttatctttc aggtatgctg gataaccggc acgatgccga tcctgaacca
      901 acc
//
LOCUS       DAZFFI010000423          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFFI010000423 DAZFFI010000000
VERSION     DAZFFI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.426"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..522
                     /locus_tag="ACHJ12_005442"
     CDS             <1..522
                     /locus_tag="ACHJ12_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993041.1"
                     /translation="AGDTSFTLAWKPGEKGQRDLIAWFDSSETRAYKIRFPNGTVDVF
                     RGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITPVTGITVTPLTGNVAKGQN
                     ITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKGVAAGKAQIPVVTGNGEFA
                     AVAEITVTDGAAG"
     gene            538..>903
                     /locus_tag="ACHJ12_005443"
     CDS             538..>903
                     /locus_tag="ACHJ12_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4993042.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPT"
BASE COUNT          222 a          230 c          266 g          185 t
ORIGIN      
        1 gccggtgaca ccagttttac tctggcctgg aagcccggcg agaaagggca gcgtgatttg
       61 attgcctggt ttgacagcag cgagacccgg gcctacaaaa tccgcttccc gaacggcacg
      121 gtggatgtgt tccgtggctg ggtgagcgcc attggtaaat ccgtgaccgc caaagaagtg
      181 atcacccgca ctgtgaaaat caccaatatc ggtcgtccgt cgctggctga ggatcgggga
      241 gaaatcacac cggtcaccgg tattaccgtg acgccactaa cggggaatgt ggcaaaaggt
      301 cagaatatca ccctgaccgt ggccgttcag ccggaaggcg caacggataa aacgttccgc
      361 gcaacctcag cgaatcagaa tttcgccacc attaccgtga aagggaacac gatcaccgtg
      421 aaaggtgttg cggcaggtaa agcgcagatc cctgttgtca ccggcaatgg tgagtttgcg
      481 gcggtggcgg agatcaccgt cacggatggc gctgcgggct gaggggggag ataaagcatg
      541 tttctgaaaa cagaacaatt tgaatataac ggggtgtccg tcacgctgtc ggagctgtct
      601 gcgctgcagc ggtttgatta tatgaagttt gtttcagacg cagaacaaca ggagacaacg
      661 aagcatgatg tcgtgcacat taaccagcga tatctggaaa cggcatccct gcttgttgcg
      721 atgtcgctat ggcattccca ttccctcaaa ggcactctgg cctctccgga gacagagatg
      781 cagcagatcc gccgtgaagt gatgctggga tggcctgctg atgcactgaa tcaggcaacg
      841 aaccgggtgc tttatctttc aggtatgctg gataaccgac acgatgccga tcctgaacca
      901 acc
//
LOCUS       DAZFFI010000424          897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFFI010000424 DAZFFI010000000
VERSION     DAZFFI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.427"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..829
                     /locus_tag="ACHJ12_005444"
     CDS             197..829
                     /locus_tag="ACHJ12_005444"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993043.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGISDEMIVELGKHSEAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKLISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDSKRTA"
BASE COUNT          284 a          162 c          203 g          248 t
ORIGIN      
        1 aacagacgga aggctctgca ggacttctgg ataattatac acgatagcca gttactgctg
       61 acctggattt attatcatac cacattattt atttcaaaaa ataaatttta aatcatttgt
      121 ttttacttgt atataaatat ccaacgatgg ttctgtttgt attatgtata ctatataaaa
      181 gagatggtga attcaaatgc gtaaaatcag tgtcagttat atatgcaaat cattatttct
      241 ctactgctca gcttctttac ctgtcttatc tgcaaatggt atttctgatg aaatgatcgt
      301 ggaacttggt aaacacagtg aggcaaaggc taatttcgct gttgctgttg gtcgatactc
      361 acaggcaaat gcagaaaaaa gtatttcttt aggtgcatca gcaagagcta ttgaaaataa
      421 ctctaccgcg ttaggagcaa acgcaacaac attgaaacca ttctctgtcg ctctgggcgc
      481 aggaagtgtt gctgatcgtg gcgctggtat tgaagcatat atgccagaga caaagcgctg
      541 gaatccaggg gatgtagtaa aatccaggat gtctgacaag cagcttaaca ggcttatgga
      601 aatcgataag ctaatatccg cttataattc acaagggacg ctgtctgctg aagagactaa
      661 acaactggag gaagtgaaaa aagagcgggc tgatatgttt gcgacatata tagcaacgca
      721 cgatgctgtg tctgtaggtg ttggtgcaac gacaacagga cgacatgctg ttacaagaca
      781 gataactagc gttgcagcag gttctgtcga tagtaagcgc acagcctgaa ccgtctggtc
      841 agaatctgac gaattagaca aagtggtgtc caccaaataa gtagtgggaa ccaaagt
//
LOCUS       DAZFFI010000425          897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFFI010000425 DAZFFI010000000
VERSION     DAZFFI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.428"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..204
                     /locus_tag="ACHJ12_005445"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACHJ12_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
     gene            complement(223..855)
                     /locus_tag="ACHJ12_005446"
                     /pseudo
     CDS             complement(223..855)
                     /locus_tag="ACHJ12_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          281 a          182 c          136 g          298 t
ORIGIN      
        1 taacccttgt ctgaacatta aaataggaac cgagatttta tataaccatt tttcacgttg
       61 tggagtaacg tggcaatgcc tggggactta taatgcgggt tttgcaatgg ataaccaaaa
      121 aaagagacag caatatgccc caaaatatac attgtatata ccaggcttaa tgaactagac
      181 aatcgtaagg cttcagcaaa atgagcctaa agcctctttt ttttatatag tgcaaacacc
      241 catacgtaaa gctgttttga tcaactcgat accactgtga acatctaatt tcttcatgat
      301 attgagtcta tgagtttcga ctgtttttct gcttaactgc aagaaattag cgatttcttt
      361 gtttgtttta ccatcagcta tgagttgtaa tatttctgac tcacgatttg taagttgatt
      421 atcagaggaa tacctggaag atattttttc acaatccata tgtacactat caaaataagt
      481 gtaaccatta ctaatcgaat caatagcacg atttagtttc gccgggtgac tggttttcca
      541 tacgcatcct ttcgcacctg cttcaagcaa ttttattgaa tcaatatacg gcatctttgc
      601 tacaaatgta ataatatgag catctatctt atgcgattta acccatttta cgagttcgga
      661 ccccatcatt ccacctgcaa attctcctaa aaaaaatatt taggctgtag atttgttaca
      721 ctccgctata gctttcttca gatctttata gcccccaaca acctcatata aatcaggata
      781 atgtccgaga aatccaatta tcccatcaat cataaacggc tgctgatctg aaactacaat
      841 tttaattttt cccatatatt catcctgagt gtaaatgctt taacgcattc atttata
//
LOCUS       DAZFFI010000426          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFFI010000426 DAZFFI010000000
VERSION     DAZFFI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.429"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..793
                     /gene="yeeO"
                     /locus_tag="ACHJ12_005447"
     CDS             <1..793
                     /gene="yeeO"
                     /locus_tag="ACHJ12_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4993044.1"
                     /translation="SRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          183 a          175 c          253 g          275 t
ORIGIN      
        1 ttctcgttat attggcgcag ttgcaatttt gtgggtgctg gcgattggtt ttaatcctgc
       61 gctaaggatt tcgttaaaga gctattttaa accgctgaat tttagcatta tctgggaagt
      121 catggggatt ggtattccag cgagtgtcga atcagtgtta tttaccagtg gtcggttatt
      181 aacccaaatg ttcgttgccg ggatggggac cagtgttatt gccggaaatt ttatcgcgtt
      241 ttcaattgcg gctcttatca acttacccgg aagtgcgctc ggctctgctt ctacgatcat
      301 tacaggccga aggttggggg tagggcagat agcgcaagca gagattcagt tgcggcatgt
      361 gttctggctg tccactcttg gattaacggc catcgcctgg ctaacggctc cctttgccgg
      421 gcttatggca tcgttttaca cccaggatcc acaggttaaa catgtcgttg tgattctgat
      481 ttggctaaat gctttattta tgcctatttg gtccgcctca tgggtgctac ccgctggatt
      541 taaaggtgct cgtgatgccc gttacgccat gtgggtttcg atgttgagca tgtggggttg
      601 tcgggttgta gtcggttatg tgctgggaat catgcttggc tggggtgtgg ttggtgtctg
      661 gatgggaatg tttgccgact gggctgtgcg ggccgtgctg ttttactggc gaatggttac
      721 tggacgttgg ctatggaaat accctcgacc cgagccgcaa aagtgtgaaa aaaagccagt
      781 tgtgtcggaa taaacgacaa aatgcagatt atttcagcaa acgatttcaa atttaaaaaa
      841 caggctttga cattgtgggt gggcatcgct aatattcgcc tcgttc
//
LOCUS       DAZFFI010000427          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFFI010000427 DAZFFI010000000
VERSION     DAZFFI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.430"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>883
                     /locus_tag="ACHJ12_005448"
     CDS             <1..>883
                     /locus_tag="ACHJ12_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4993045.1"
                     /translation="GLAVGLLSGFNTAYRGAIHAPSASATMNNTWWQLTGDSSLRSLK
                     NTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNQKLSGKDNILL
                     VDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAKDAGDKTTWNLT
                     GYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGAWARIMSGTGS
                     AGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGKTKSVGAGLYA
                     SAMFDSGAYIDLIGKYVH"
BASE COUNT          252 a          199 c          229 g          203 t
ORIGIN      
        1 ggactcgcgg taggattact gagtggtttt aatacggcat accgcggtgc aattcatgcc
       61 ccgtcagcat ctgccactat gaacaacacc tggtggcaac tgacaggaga ctcctcactt
      121 cgctcgttaa aaaataccgg aagcatgaca tattttacag gaagtgcagc gaataaagca
      181 ttccatacac tgacggttga tgagctgacg acgaatggca ctgcgtatgc catgcgtacg
      241 gacctgaaaa atgcggataa gctggtagta aaccaaaagc tgtcaggtaa ggacaatatt
      301 ctgctggttg attttctgaa caaacccacc ggagaaaaac tggatattga actggtgagt
      361 gcaccgggga acagcagtaa ggatgttttt aaaggaagtg aacaggcaat aggtttcagc
      421 aatgtcacac ctgttattac agctaaagac gccggagata aaacaacatg gaacctgacc
      481 gggtacagga tggcagaaaa tcccgccgca acccaaagtg cctcaggcct tgcatctgtg
      541 gggtacaaat catttttgag tgaggtcaac aacctgaata aacgtatggg tgacctgcgt
      601 gacatcaatg gtgaagctgg cgcatgggca cgtatcatga gcggaaccgg ctctgccggt
      661 ggtggtttca gtgacaacca cacacatgtt caggtcggtg tcgacaaaaa acatgagctg
      721 gacggactgg atttgtttac cggcttcact gtcacacaca ctgacagcag tgcctctgct
      781 gatgctttca aaggtaaaac aaaatctgtg ggggccggac tctatgcttc cgccatgttt
      841 gattccggtg cctatatcga cctgattggt aagtatgttc atc
//
LOCUS       DAZFFI010000428          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFFI010000428 DAZFFI010000000
VERSION     DAZFFI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.432"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..675
                     /locus_tag="ACHJ12_005449"
     CDS             <1..675
                     /locus_tag="ACHJ12_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFO4993046.1"
                     /translation="LFAVSITSSGEPPTFSLTPDGKLTARNADISGNINANSGTLNNV
                     TIAENCVVKGTISADRIIGDIVKGYSFEPRDGTLHITNHKYGEGYVIFKAEVSLADDV
                     SFDRKLILLPFTFYSGTLTIEINGRVVIKADPRVANTGTAGYFDRYDNPVYTRTNLGN
                     TVSGVWDVAAGGGDTRITVTMRVEGGPGNMGDVEGRKRELVRWRYDAFTLMVAKSTDS
                     GFTRRS"
BASE COUNT          235 a          188 c          251 g          198 t
ORIGIN      
        1 ctgtttgcgg tgagtattac ctcatcaggt gagcctccga cgttttccct gacgccggac
       61 gggaaactga cagcccgtaa tgcggatatc agcgggaata ttaatgcgaa ctcgggcacg
      121 ctgaataacg tgaccattgc agaaaactgc gttgttaaag gaaccattag cgcagacaga
      181 atcatcggtg atatcgttaa gggctattcc tttgaacctc gtgatggcac gctccatatt
      241 acgaaccaca agtatggaga gggttatgtc atctttaagg cggaggtatc tcttgctgat
      301 gatgtttcgt ttgatcgcaa gctgatactc cttcccttca ctttctattc aggaacactg
      361 acgatagaga ttaacggaag agttgtcatt aaggcagacc caagagttgc taatacggga
      421 acggctggtt attttgacag atacgacaat cctgtttaca cgcgcaccaa cctcgggaac
      481 accgtttcag gagtatggga tgtggcggca ggagggggag atacacggat tacagtgaca
      541 atgagagttg aaggtggtcc tggcaatatg ggggatgtgg aagggagaaa gagagagctg
      601 gtgcgatgga gatatgacgc ctttactctc atggtggcga aatcgacaga ttccggtttt
      661 acccggagat cgtgacaggc agccggaata cggattgccg gaaggagaca aaaaccgtac
      721 attatgcgcg ggtgcctttg gctgatggcc ggagggaaca cctgaaggcc ggaaagacga
      781 aggccccgga caaacattca tgtttaaccc gaggcctgac gttcttcctt cagcaagaag
      841 aaggttagcg cctctccgta aaaggagcaa gc
//
LOCUS       DAZFFI010000429          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFFI010000429 DAZFFI010000000
VERSION     DAZFFI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.433"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..109)
                     /locus_tag="ACHJ12_005450"
     CDS             complement(<1..109)
                     /locus_tag="ACHJ12_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993047.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKI"
     gene            303..728
                     /gene="yqeK"
                     /locus_tag="ACHJ12_005451"
     CDS             303..728
                     /gene="yqeK"
                     /locus_tag="ACHJ12_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO4993048.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVELDSWICTRCC"
     gene            764..844
                     /locus_tag="ACHJ12_005452"
     CDS             764..844
                     /locus_tag="ACHJ12_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993049.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          273 a          125 c          161 g          312 t
ORIGIN      
        1 tgattttaac tggattaacg gatttctctt catttttctt atttttgttc tcctgatcag
       61 gaactaatgg ttgcagtggt aggctattat taatatttcg tggcttcatt ttacatttct
      121 ctcatttgat tagagcaaaa tccgaattcg cagcttctga taatgtcaaa tatcacctta
      181 tggagacgtt acgtagaatg actatatttg ttatcggcac attaggggta ttaattttag
      241 tattgatgga aggtgattat tgaacagtaa catcgagtca cggtaaaaat gtgaggtata
      301 taatggacat tgagttttcg cagattcatg aaatggtcta tatgcatgat attgtaaatt
      361 cggactcgaa aaagaaacca aggattccgt taaaaaaatt ccttaatgct gagaaggttc
      421 tcacgcaaac aacatcatgg gcattaaatt caagatttgt gaatgttaat tctgtcaata
      481 aagtcaacgt aaagagtaaa gttaaaagca gttatatctc acgatctgtc aatgatgagt
      541 ttagtttaac agatgatgaa atcaattctt ttaaagaaac gttagtcctt tcctctattg
      601 attcactgag taaactggtt cttaataatc ctctttctgt attgtttaca tcgactgtac
      661 gaaggaataa taatcgagct aagatgaatg tcgagctcga ctcatggatt tgcaccagat
      721 gttgttgata catgatattt tacattgcaa ggagatgtct aaaatgaaag atgttgatca
      781 aaactttgat gctttagact gccatatact gcgagaatat ttaattttat tgttttatga
      841 ttaacgaggt tccatctttt tgttatctat t
//
LOCUS       DAZFFI010000430          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFFI010000430 DAZFFI010000000
VERSION     DAZFFI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.434"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..856
                     /locus_tag="ACHJ12_005453"
     CDS             47..856
                     /locus_tag="ACHJ12_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4993050.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
BASE COUNT          287 a          169 c          168 g          243 t
ORIGIN      
        1 caaactatgt gatctccatt tcgattgatt tagtgtttat tgacgtatgt actggattat
       61 taacgataat atcgagttct ggcctgagca ccgaaaatta atatcggtac ataacgccga
      121 tcttaacgtc gttctgacaa cgccagccag tcgatgttta tcacttctac ttgaagcttt
      181 tcctgatgtg gttgcacaac aagatttttt caccagagtc tgggaagaag aaggtatgcg
      241 tgtgcctact aacacgttat atcagaacat atccattatc agacgcggat ttcgcgctgt
      301 tggtgatact acccactcgc taattgcaac cgtgccgaga agaggattca agatccataa
      361 tgacattaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata cacacaatgc
      421 cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg caaagaattt
      481 caataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg catttgtcat
      541 aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat ttttcaagga
      601 ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag atacaattga
      661 cgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga ttaactgtaa
      721 aaaacatccg tggttatact tccctcttgc aaaatcctca ccggggatga ttgttatggc
      781 atgcaataaa aattataacc aacatgaagt ggcagactgt ttaaccctgt cttaccgtga
      841 ggttaatcgt gattgattat aaaaaaa
//
LOCUS       DAZFFI010000431          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFFI010000431 DAZFFI010000000
VERSION     DAZFFI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.435"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHJ12_005454"
     CDS             complement(<1..229)
                     /locus_tag="ACHJ12_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4993051.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEG"
     gene            complement(234..>848)
                     /locus_tag="ACHJ12_005455"
     CDS             complement(234..>848)
                     /locus_tag="ACHJ12_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO4993052.1"
                     /translation="LIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFV
                     VDEAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCL
                     TMPPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLA
                     LGMTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
BASE COUNT          185 a          294 c          186 g          183 t
ORIGIN      
        1 gaccttccag catcaccgcc acactgctgt tcagacccac cctctgtggc tggcaacgga
       61 tacactccac ccgccccagt tgcgccgcca gatgcccgcg aagacgttca agattatccc
      121 gtgtggtgca acttatcccc ggcgcaccct gtggcggcag aacaaacaca cttccggcgg
      181 ggtgaacagc agacaccact tccggttccg gatacttcag acacagcata gtctcactcc
      241 ctgccagcca gaccacgtag cccgtccagc tttcgtgcca catgaaacgc tgcttccagt
      301 tcgctgcact ggtgcacctt catcagggcg cggcgttcag ccttctcttc tttctccgtc
      361 atccccagag ccagatacag actcggtggc acaacccgga acaacgcttc tgtgcgcttc
      421 gccagtacca ccccctccgt ataacagccg gacagtttac tggctgacag cagtaccgcc
      481 ttctgctcct ccgtcaggga tttaaaacgg gcaatattgt tcacttcctc cggcggcatg
      541 gtcaggcaca cccaccactc tgccatattc agcatcttct ccgagacatc cggataatcc
      601 ttcaggttct gcgtggccag ccacagccag gcccccagct tgcgccacat tttcaccact
      661 ttggtcatat acggggacaa aagcgggttt accgtcacga tatgcgcctc atccaccaca
      721 aacagaatat cccgtcccag atactgatcg cgctcggcaa tgttgttaat catattggtc
      781 attgacacca tggtcagtgc catctgggct tcataacctt cacgggcaag atgccccaga
      841 tcaatcag
//
LOCUS       DAZFFI010000432          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFFI010000432 DAZFFI010000000
VERSION     DAZFFI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.436"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..229)
                     /locus_tag="ACHJ12_005456"
     CDS             complement(<1..229)
                     /locus_tag="ACHJ12_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4993053.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGQVECIRCQPQRVGLNSSVAVMLEG"
     gene            complement(234..>848)
                     /locus_tag="ACHJ12_005457"
     CDS             complement(234..>848)
                     /locus_tag="ACHJ12_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO4993054.1"
                     /translation="LIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFV
                     VDEAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCL
                     TMPPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLA
                     LGMTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLSGRE"
BASE COUNT          189 a          290 c          180 g          189 t
ORIGIN      
        1 gaccttccag catcaccgcc acactgctgt tcagacccac cctctgtggc tggcaacgga
       61 tacactccac ctgccccagt tgcgccgcca gatgcccgcg cagacgttca agattatccc
      121 gtgtggtgca acttatcccc ggcgcaccct gtggcggcag aacaaacaca cttccggcag
      181 ggtgaacagc agacaccact tccggttccg gatacttcag acacagcata gtctcactcc
      241 ctgccagaca gaccacgtag cccgtccagc tttcgtgcca catgaaacgc tgcttccagt
      301 tcgctgcact ggtgcacctt catcagggcg cggcgttcag ccttctcttc tttctccgtc
      361 atccccagag ccagatacag actcggtggc acaacccgga acaacgcttc tgttcgcttc
      421 gccagtacca ccccctccgt ataacagccg gacagtttac tggctgacag cagtaccgcc
      481 ttctgctcct ccgtcaggga tttaaaacgg gcaatattgt tcacttcctc cggcggcatg
      541 gtcagacaca cccaccactc tgccatattc agcatcttct ctgaaacatc cggataatcc
      601 ttcaggttct gcgtggccag ccacagccag gcccccagct tgcgccacat tttcaccact
      661 ttggtcatat acggggacaa aagcgggttt accgtcacga tatgcgcctc atccaccaca
      721 aacagaatat ctcgtcccag atactgatcg cgctctgcaa tgttgttaat catattagtc
      781 attgacacca tggtcagtgc catctgggct tcataacctt cacgggcaag atgtcccaga
      841 tcaatcag
//
LOCUS       DAZFFI010000433          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFFI010000433 DAZFFI010000000
VERSION     DAZFFI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.437"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>842
                     /locus_tag="ACHJ12_005458"
     CDS             <1..>842
                     /locus_tag="ACHJ12_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO4993055.1"
                     /translation="NVAAVASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVA
                     RRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQG
                     GIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEE
                     LIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNP
                     DADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGL
                     PVIVS"
BASE COUNT          164 a          219 c          272 g          187 t
ORIGIN      
        1 aatgtggccg cggtggcttc tgcgccggtg atccgtgtgg agcagaaact ggataagggg
       61 attggtttcg cacgttttgc caaatcgctg gctgcggcta aaggtgtccg ctctgaagcc
      121 ctggaagtgg cccgtcgtca gtatccggat gacagtcgtc tgcatcatgt cctgaaatcg
      181 gcagtgggcg cggggaccac cacggatccg cagtgggcag gcagcctgtc tgaatatcag
      241 gaatacgcac aggactttat tgattacctg cgtcctcaga ccattatcgg gcgatttggt
      301 cagggtggga tccctgcact tcgtcaggtg ccgttcaata tccgtgtgca cgcccaggtg
      361 tccggcggtg ctgccggctg ggtgggtgag ggtaaggcaa aacccctgac gaagtttgat
      421 tttgaatcca tcaccttcag tcatgcgaag gtgtcggcca ttgcggtact gacggaagaa
      481 ttgatccgtt tttccagtcc ggctgctgat gcactggtcc gtaatgcgct ggcggaagcg
      541 gtggtggcgc gtctggatac agactttgtg gacccgaaaa aagccgcagt ggcagatgtc
      601 tccccggcgt ccatcaccca tgatgtgaag ggcacggcat caagcggtaa cccggatgcg
      661 gatgcagagg ctgcgtttgg tcagtttgtg gcagcaaacc tgcagcccac cggtgcggtc
      721 tggctgatgt ccagcaccaa tgccctggca ctgtccatgc gtaaaaatgc gctgggtcag
      781 aaagaatacc cggacatgac cctgctgggg gggaccttcc aggggctgcc ggtgattgtc
      841 tc
//
LOCUS       DAZFFI010000434          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFFI010000434 DAZFFI010000000
VERSION     DAZFFI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.438"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..573
                     /locus_tag="ACHJ12_005459"
     CDS             <1..573
                     /locus_tag="ACHJ12_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993056.1"
                     /translation="RRAVPRASATTLNRVAGAIIAKTASSVARELAVPRRLIRARIRL
                     SPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQRAALKGGGSVLIVGKRR
                     IPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLPIEVVKIPTAGPLAETFE
                     REQDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            586..>842
                     /gene="gpU"
                     /locus_tag="ACHJ12_005460"
     CDS             586..>842
                     /gene="gpU"
                     /locus_tag="ACHJ12_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4993057.1"
                     /translation="MKHREIRAAILSALKANISERVTWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEATLHIEVFLRAKETDSVLDMWMEEK"
BASE COUNT          198 a          210 c          270 g          164 t
ORIGIN      
        1 cgccgtgccg ttccccgggc ctctgccacg acactgaacc gcgtggcggg ggccattatt
       61 gcgaaaacgg cctcttcagt tgccagggag ctggccgttc cccgtcgtct tatccgtgcc
      121 cgcatccggt taagtccggc acgaccggat aaggtttacg caaaggttta catcaatacc
      181 ggcaacctgc ccgccatcaa actgggggag gcccgcgttc gactttcccg cagaaaacgg
      241 agaaagaaag gacagcgtgc ggccctgaaa gggggcggca gtgtgctgat tgtggggaaa
      301 agacggatcc cggacgcctt tatcacccgg ctggctaacg gacgctggca tgtgatgcag
      361 cgtatgccgt gggcatcatc gtccaccggc gcggacagca aagggaggcc gaaacgccac
      421 cgtctgccga tcgaagtggt gaaaattccg actgccggac cgctggcaga aacctttgaa
      481 cgtgaacagg accggatgta ccgggaaaaa ttaccggcgc agatgatgac ggccatggcg
      541 catcagttac gcctggtgct gaaaagaaaa tgacagggag ggtgtatgaa acaccgtgaa
      601 atacgggcgg caatattgtc tgccctgaaa gcaaatattt ctgagcgggt gacctggttt
      661 gatggtcgtc cggtatttat tgatgaacag gaactgcctg cggtggcggt ttatctgaca
      721 gatgcttctg cgacggagga gtttgttgac gaggggacct gggaggcgac actgcatatt
      781 gaggtgtttc tcagggcgaa agagacggac tccgtgctgg atatgtggat ggaagaaaaa
      841 at
//
LOCUS       DAZFFI010000435          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFFI010000435 DAZFFI010000000
VERSION     DAZFFI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.439"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..576
                     /locus_tag="ACHJ12_005461"
     CDS             <1..576
                     /locus_tag="ACHJ12_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993058.1"
                     /translation="RRAVPRAAATTLNRVAESIIAKTASSVARELAVPRRLIRERIRL
                     QRASADRVYAKVIINTGNLPAIKLGTASVLLSRRKRRKKGERSVTKGGGSVLIVGKRR
                     IPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLPIEVVKIPTAGPLAETFE
                     RERDRMYREKLPVQMMKAMTHQLRLVLKRKS"
     gene            586..>842
                     /gene="gpU"
                     /locus_tag="ACHJ12_005462"
     CDS             586..>842
                     /gene="gpU"
                     /locus_tag="ACHJ12_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4993059.1"
                     /translation="MKHREIRAAVLSALKENISERVNWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEK"
BASE COUNT          197 a          210 c          271 g          164 t
ORIGIN      
        1 cgccgtgccg ttccccgggc tgccgccacg acacttaacc gtgtggcgga gtccatcatc
       61 gcgaaaacgg cctcttcggt tgccagggag ctggcggttc cgcgccgtct catccgtgag
      121 cgtatccgcc tgcaacgggc cagcgcagac agggtttatg cgaaggtcat catcaacacc
      181 ggtaatctgc ccgccataaa actggggacg gccagcgtgc tgctttcccg cagaaagcga
      241 cgaaagaaag gcgagcgttc ggtcacgaaa ggcggtggca gcgtgctgat tgtggggaaa
      301 agacggatcc cggatgcctt tatcacccgg ctggctaacg gacgctggca tgtgatgcag
      361 cgtatgccgt gggcatcatc gtccaccggc gctgacagca aagggaggcc gaaacgccac
      421 cgtctgccga tcgaagtggt gaagattccg actgccggac cgctggcaga aacctttgaa
      481 cgtgaacggg accggatgta ccgggaaaaa ttaccagtgc agatgatgaa agccatgacg
      541 catcagttac gcctggtgct gaaacgaaaa tcgtaaggag ggaatgtgaa acaccgtgaa
      601 atacgggcgg cagttctgtc tgccctgaaa gaaaatattt ctgagcgggt gaactggttt
      661 gacggccgcc cggtatttat tgatgaacag gaactgcctg cggtggcggt ttatctgaca
      721 gatgcttctg cgacggagga gtttgttgac gaggggacct gggaggccgt gcttcatatt
      781 gaggtgtttc tcagggcgaa agagacggac tccgtgctgg atatgtggat ggaagaaaaa
      841 at
//
LOCUS       DAZFFI010000436          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFFI010000436 DAZFFI010000000
VERSION     DAZFFI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.440"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..>841
                     /locus_tag="ACHJ12_005463"
     CDS             91..>841
                     /locus_tag="ACHJ12_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993060.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYK"
BASE COUNT          272 a          169 c          184 g          216 t
ORIGIN      
        1 tttgttatta tttaagtggg gtatataatt agagtccgtt atcaatgcta aatattctaa
       61 tcattatgac aggcgaggga gtgtccaatt atgaattcaa aaaagctttg ttgcatatgt
      121 gtgttattct cgctgcttgc aggatgtgcc tctgaatctt ctattgatga aaagaagaaa
      181 aaagctcaag tcacacaaag taatattaat aaaaacactc cccagcaact gacagacaaa
      241 gatttattcg gtaatgaaac cactctggcc gtatccgaag aggatattca agctgcgctt
      301 gatggagatg agttccgcgt tccccttaat tctcctgtaa tacttgttca atccggcaac
      361 cgcgcaccgg aaaccattat gcaggaggag atgcgaaaat attatactgt ttccacattc
      421 tctggtatcc cggacaggca aaagcctctg acttgtaaca aaaacaaaga taaaaatgag
      481 aacgaggatg ttgccagtgc tgagaatatg aactggatgc aggcactgcg ttttgtggct
      541 gcaaaaggac atcagaaagc gattattgtt taccaggata cattgcagac aggaaaatat
      601 gactctgcgc tgaaatcaac agtatggtcc gactataaaa atgaaaaact caccgacgct
      661 atctccctgc gctacctggt acgtttcacg ctggtggatg tggcaacagg tgagtgggct
      721 acctggtcgc cggtgaatta tgaatataaa gtgcttccac cattgcccga caagaatgaa
      781 gccagtacga ctgatatgac agagcagcaa atcatgcaac ttaagcaaaa gacctataaa
      841 g
//
LOCUS       DAZFFI010000437          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFFI010000437 DAZFFI010000000
VERSION     DAZFFI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.441"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>838
                     /locus_tag="ACHJ12_005464"
     CDS             <1..>838
                     /locus_tag="ACHJ12_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4993061.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT
                     GPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRY
                     GQLLSFTDCSGYQTRYGHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRY
                     EYN"
BASE COUNT          209 a          245 c          258 g          126 t
ORIGIN      
        1 cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg cataaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 accgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacctcccc
      601 tgcgcaacgg acgatgccac cggcagccgg aaaaccatga cgtggagccg ttacggccag
      661 ttgctgagct tcaccgactg ttccggctac cagacccgtt atggccatga ccgcttcggg
      721 cagatgacgg cggtgcaccg cgaggaaggg ctgagtcagt accgcgcata cgacagccgt
      781 ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga atacaacg
//
LOCUS       DAZFFI010000438          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFFI010000438 DAZFFI010000000
VERSION     DAZFFI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.442"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>838
                     /locus_tag="ACHJ12_005465"
     CDS             <1..>838
                     /locus_tag="ACHJ12_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4993062.1"
                     /translation="RGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSAT
                     GPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRY
                     GQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAFDSRGQLIAVKDTQGHETRY
                     EYN"
BASE COUNT          210 a          233 c          258 g          137 t
ORIGIN      
        1 cgcggcgaac tggcggtggt gtatgaccgt agtggcaaac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccggcc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagttac
      181 acgtatcagt atgaaaaaga ccacatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgatgcggtg ggcaggctta aggcacagac ggatgccgca
      361 ggccggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc
      481 gggccagacg ggctggaaat gcgccgcaaa tatgatgagt acggacgtct gattcaggaa
      541 accgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacctcccc
      601 tgcgcaacgg acgatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag
      661 ttgctgagct tcactgactg ttccggttat gtaacccgct atgaccatga ccgttttggt
      721 caggtgacgg cggtgcaccg cgaggaaggg ctgagtcagt accgcgcatt cgacagccgt
      781 ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga atacaaca
//
LOCUS       DAZFFI010000439          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFFI010000439 DAZFFI010000000
VERSION     DAZFFI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.443"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>834)
                     /locus_tag="ACHJ12_005466"
     CDS             complement(<1..>834)
                     /locus_tag="ACHJ12_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4993063.1"
                     /translation="VGVFGPGWKAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEIS
                     YSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPPPPAYRVLTGVVDGFGRTLAFHRAAKGDVAGAVTGVTDGAGRRFHLA
                     LTTQAQRAEAFRKQRASSLSSPASPRSVSSSLVFPDTLPAGTGYGTDNGIRLEAVWLT
                     HDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRP
                     ES"
BASE COUNT          145 a          304 c          251 g          134 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgcg ttccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatccgtg ccgtatcctg taccggcggg
      241 cagcgtgtcg gggaaaacca gagaagagga aacagaacgg ggactggccg gggaagataa
      301 ggaagaggcg cgctgtttac ggaaggcttc cgcccgctgc gcctgcgtgg tcagcgccag
      361 gtgaaaacgg cggcccgcgc cgtccgtcac ccccgtcacc gcgcccgcca cgtcaccctt
      421 agctgcccgg tgaaaggcca gcgtccgccc gaagccgtcc accacccccg tcagcacccg
      481 gtatgcgggc ggcggaggcg gcagcacctc gtccgccccc ggcacccgct ccggccagct
      541 gagtatccac cacggcccct gaaggctgtt tgtcgccagg tacacatgcg gactcaggcg
      601 aacatcttcc ggcagcacct gccagagcgc gcttagcggc tgcgaactgt gctgcgccgc
      661 tactccgccc cgcgccagcc agagtgactc gctgcggctg tagcttatct cgccgggaaa
      721 cagcggctca aagtgaatgc tcctgccgcc gctgtcgttg agtatcaggc cttcatcgcg
      781 tatctgtaag cggatatcga agggcgcttt ccagccggga ccaaacaccc ctac
//
LOCUS       DAZFFI010000440          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFFI010000440 DAZFFI010000000
VERSION     DAZFFI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.444"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            78..266
                     /locus_tag="ACHJ12_005467"
     CDS             78..266
                     /locus_tag="ACHJ12_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4993064.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            646..>827
                     /locus_tag="ACHJ12_005468"
     CDS             646..>827
                     /locus_tag="ACHJ12_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993065.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAG"
BASE COUNT          248 a          137 c          234 g          208 t
ORIGIN      
        1 agacgctcac tttttttcgc atgcattacc ggagtgatgg ctcagccggt gattttgttg
       61 ttgagatgag agccagtatg ggaaagcgat ataaaataac tgcgacaata atcaaaccag
      121 gcaatatgcc ggttcagtgg ctgcgttacc aggatgaaaa aatgactcag gatgaatgtg
      181 aaaaactgtt tgcgcccgca aaagagccgg gacggtatat tggcgtcagg gtggaggact
      241 tccggtgtga agtggtgaag gcctgagtat gaaaataaaa acctccggtg gagtcggagg
      301 gggcaaaaac agatagtccc ggagtgtatc tgtgtaaatg gtgttattgg tataataatg
      361 gtggtaaaga ccacgggaga atttattcat tttattcgtc atgaaatcaa cggtaatagt
      421 gtgtttctgt gacgggaata aaaaatacag cacagccgat gacccggtgc tgaaaacagg
      481 ttaaatgtag tgggcacatt tccggtaata tcaggaacag catgttatgg ttataaaagc
      541 atataccggg agtgtgccct gacactgtga tgttctggtt gtcttttgac catttcccgg
      601 ggacgaaata taagctgttc tgcgttaaca cttaataatg agagattgta tgctgaaaaa
      661 acaattctgg cgttatgtat ttccgcacca tttctggttg cgggctgtga tgatgcgaca
      721 aaggagaatg taagtaagaa ggcggcagat ataaaggaag ctgcgcagac tcaactcagt
      781 gatgtgaagg atgccgcgga ggaagtgaaa atgcaggctg ccggaaa
//
LOCUS       DAZFFI010000441          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFFI010000441 DAZFFI010000000
VERSION     DAZFFI010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.445"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHJ12_005469"
     CDS             complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHJ12_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO4993066.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCW"
     gene            complement(750..>825)
                     /locus_tag="ACHJ12_005470"
     CDS             complement(750..>825)
                     /locus_tag="ACHJ12_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO4993067.1"
                     /translation="LEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          239 a          157 c          164 g          265 t
ORIGIN      
        1 tgccaacaat ctgaagtaat gctggtttta ttaaagtgcg caggaggtgt taagatggct
       61 tcttttcggg tgaattttgc acaatactta gaaatcgtct ccttaataca ttccagctct
      121 tttttattga cggctgagca agtacattgt tcaccaaaag gtgcaaaagt gcctacatct
      181 aatgtttgta tgatataatc gtcatgttct cctgtagaaa tatctatgat gaatggactc
      241 atattttccg gttggattcg gatcatgaca gaaggaacat cgccttgata gaaattcaat
      301 gcagtaattg cagataatac tctccgcagt tcattactta tactgtaaat tttattaacg
      361 tcattggtgg agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttcta
      421 ttgtgattgt taaccactgc gtgcattggc gggattgaag ggtgaaaaat atgtgcaatt
      481 gggaagcctt tcattgatgc ctgccttgtc gtcatgacaa acaataatag atgcttattt
      541 tcctcgcagg gttctttcgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac
      601 gaagcaacat tctgtggcct acgacgatca aaagctctgt gaaaatttcc cttgttgtct
      661 ttgtctgacc ttatcgcggc gaccagcaat tattcgtttt gtgatcctca acacaaaatg
      721 ctattcgtcg cctatgaggg gagaagaatt tatggaaaac gattgcattc acaaaatgac
      781 gtcctgtcag aagagaaaaa gttgacgtta atcaattgtt cgaga
//
LOCUS       DAZFFI010000442          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFFI010000442 DAZFFI010000000
VERSION     DAZFFI010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.446"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..66
                     /locus_tag="ACHJ12_005471"
     CDS             <1..66
                     /locus_tag="ACHJ12_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4993068.1"
                     /translation="CYGQVSRRDQESALACWGIDR"
     gene            585..770
                     /locus_tag="ACHJ12_005472"
     CDS             585..770
                     /locus_tag="ACHJ12_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4993069.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          207 a          185 c          237 g          193 t
ORIGIN      
        1 tgctatggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggtatcgac
       61 agataagcag aatattttgc tgaaaaatga cgttggccaa cgcggacgga taacacgaaa
      121 tcctgcgaac tggcaaaacc taagtgaata aaagtaaaaa ccccgtttgt tagcagcagg
      181 tggggttttg tgtttcctga ctccggaaaa gtcaaaggag aaagtgtgtt tgattttagc
      241 aaactgattc gggagatttg agtgatggct gaaaaattat ctacctggaa gttcattctt
      301 atctggctgg tgtttgtgat tatggtctcc ggttatttca tcggtcagat acgctggtgg
      361 tgaaatgaac cgcgttctgt gtgtggtcat cattgccctg ctggtggcct gtggtgcgct
      421 tagtctgggg ctgaatcatt accgtgataa cgccatcacc tacaaagcgc agcgcgataa
      481 aaaagccaga gagctggagc tggcaaacgc aaccattact gatatgcagg tgcgccagcg
      541 cgatgttgct gcgctcgatg caaaatactc gagggaatta gctgatgcga gagctgaaaa
      601 tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg
      661 ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa ccggcccccg
      721 actggcagac accgctgaac gggattattt catcctcaga gaacggttga tgacaatgca
      781 gaagcagctg gaaggggcac aggactatat ccgcactcag tg
//
LOCUS       DAZFFI010000443          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFFI010000443 DAZFFI010000000
VERSION     DAZFFI010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.447"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..273
                     /locus_tag="ACHJ12_005473"
     CDS             <1..273
                     /locus_tag="ACHJ12_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4993070.1"
                     /translation="HLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQK
                     YGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            535..>821
                     /locus_tag="ACHJ12_005474"
     CDS             535..>821
                     /locus_tag="ACHJ12_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993071.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMR"
BASE COUNT          285 a          135 c          158 g          243 t
ORIGIN      
        1 catctaatta gaagcaatgc tatagcatcc acacctgtat atgacaatta ttttaataac
       61 actactgctt tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa
      121 actaaacaga aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat
      181 tataccatgg ggctaaattc ctggaagata gctcctaacg aacgtggtga taaaaaaggt
      241 gtcccagggc ttagttgttt tcaaccacat taaaactcca tgagtgctgt ggtatatctt
      301 tttaatcctg ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga
      361 cgcctctaca atctttaggg actttctctg ctatgagata agaaatttca tattatgctt
      421 taaggctgaa tgatttgtgc aaaatataat tatttttcca tcgaacatga ggtgtatgtc
      481 ataagattaa atgttaacta tgcattcctc gcggtgaatt atatgaggta aaagatgaaa
      541 attggaactg tggcaggcac taacgacagt acaacgacaa tcgcaacaaa tgatatggtg
      601 caagagcatg ttactaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt
      661 gatgatatat caagtattgc caggacaatg ctcggtgaag ttgtggaaaa aattgatgat
      721 tggcaaatag aacgtttcca acaatctatt cgagatgaca aaatatcatt taccatacaa
      781 acgaatcatt cagaaaaata tagtatgcta tcggacatgc g
//
LOCUS       DAZFFI010000444          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFFI010000444 DAZFFI010000000
VERSION     DAZFFI010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.448"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>819
                     /locus_tag="ACHJ12_005475"
     CDS             <1..>819
                     /locus_tag="ACHJ12_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative coupling factor TraD, PFGI-1 class"
                     /protein_id="HFO4993072.1"
                     /translation="LLRRDSPLNPVRPLPPVGGNPALHGIEPDEQDVTLALGERVGHT
                     IVYGTTRVGKTRLAELLVTQDIRRGDVTIVFDPKGDADLLLRVWAEAHRAGRGDELYI
                     FHLGWPEISARYNAVGRFGRVSEVASRVAGQLSGEGNSAAFREFAWRFVNIVARALVA
                     LGERPDYTLIMRYVNNIADLYIRYAGKVISEQMPELENAILNNMNVLGEADVPRTMQN
                     QPDAVRIWAIEVALSSEAGKKLYDPILDGLRSAVRYDRTYFDKIVASLLPLLEKL"
BASE COUNT          160 a          195 c          259 g          205 t
ORIGIN      
        1 gcttcttcgt cgtgactcgc cgctgaatcc cgtccgtccc ctgccgccgg tgggaggaaa
       61 tcctgccctg catggtattg agccggatga gcaggatgtg acgctggcac tgggtgaacg
      121 tgtggggcac accattgtct atggcaccac ccgtgtcggg aaaacccggc tggcggagtt
      181 gctggtcaca caggatattc gtcggggaga tgtcaccatt gtctttgatc ccaaagggga
      241 tgcggatctg ttgctgcgtg tctgggctga agcacaccgt gccggcaggg gcgatgagct
      301 ttatatcttt catctgggct ggccggaaat ctctgcgcgt tataacgccg tggggcgttt
      361 tggtcgtgtg tcggaggtgg cttcccgtgt ggccggtcag ttatccggtg aaggtaacag
      421 tgcggccttc cgtgagtttg catggcggtt tgtcaacatt gttgcccgtg cactggtggc
      481 actgggggag cgcccggatt acacgctgat tatgcgttat gtgaataata ttgccgacct
      541 ttatatccgt tatgctggta aggtgatcag cgaacagatg ccggaactgg aaaacgccat
      601 tctgaataac atgaatgtac tgggggaagc ggatgttccc cggaccatgc agaaccagcc
      661 ggatgctgtg cgtatctggg ccattgaggt ggctctcagc agtgaagccg gaaagaaact
      721 ctatgatccg atactggatg gtctgcgaag cgcggtacgt tatgatcgta cctattttga
      781 caaaattgtg gcatccctgt tgccgttgct ggaaaaact
//
LOCUS       DAZFFI010000445          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFFI010000445 DAZFFI010000000
VERSION     DAZFFI010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.449"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..486
                     /locus_tag="ACHJ12_005476"
     CDS             52..486
                     /locus_tag="ACHJ12_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO4993073.1"
                     /translation="MNDNALVDVDDAVVARIRDVLAYTFPGQKFSVRKCDPSFPKVFN
                     NVWVSWLDGPTEAEVRSVTEGFENKDGLLSVLESRYFSDELVQECIDRLRKEYGQGNV
                     PLSVTVERYRKNELEKIETARFPGNISVAINEIGINTSKYRR"
     gene            490..804
                     /locus_tag="ACHJ12_005477"
     CDS             490..804
                     /locus_tag="ACHJ12_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993074.1"
                     /translation="MAGKCLVWFCNGISEPCLIAGNISELLADVTITSNYRGDVTSEI
                     RDVVQEWLRTGRGNLADLKEELWYYNLFINPSADELMNVNRRYGPERTAWLQGFIRNN
                     GA"
BASE COUNT          208 a          166 c          241 g          198 t
ORIGIN      
        1 cctcacgggg cagtgctctc tgttatttca ggattacaga ggaactgagt gatgaacgat
       61 aacgcactgg ttgatgttga cgatgctgtt gtggcccgta tcagggatgt gctggcgtat
      121 acatttccgg gacagaaatt ttctgtgcgg aaatgcgatc catctttccc gaaagttttc
      181 aacaatgtgt gggtgagctg gctggatggt ccaacggagg ccgaagtacg ctctgtcaca
      241 gaggggtttg agaataaaga cgggctgctg tctgtgcttg aatcccgtta tttcagcgat
      301 gaactggtac aggagtgtat tgatcgcctt cgtaaagagt acggacaggg gaacgtcccg
      361 ttgagtgtga ccgtggagcg ttaccggaaa aacgagctgg agaagattga aacagcgcgt
      421 tttccgggca acatatccgt agccataaac gagatcggga taaataccag caaatacagg
      481 aggtaaggta tggctggtaa atgccttgtc tggttctgta acgggatctc tgaaccgtgt
      541 ctgatagcag gaaatatcag tgaattactg gcagatgtta cgatcacgtc aaattaccgt
      601 ggagatgtca catctgaaat cagggatgtt gtacaggagt ggctcaggac cggcaggggg
      661 aacctggcgg atttaaagga agagctctgg tactacaacc ttttcatcaa tccgtctgca
      721 gacgaactga tgaatgtcaa caggcggtat ggccctgagc gaactgcctg gctgcagggt
      781 tttatccgca acaacggggc gtgatacccc gtt
//
LOCUS       DAZFFI010000446          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFFI010000446 DAZFFI010000000
VERSION     DAZFFI010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.450"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..224
                     /locus_tag="ACHJ12_005478"
     CDS             24..224
                     /locus_tag="ACHJ12_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4993075.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALQHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(356..682)
                     /locus_tag="ACHJ12_005479"
     CDS             complement(356..682)
                     /locus_tag="ACHJ12_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO4993076.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
BASE COUNT          258 a          205 c          153 g          195 t
ORIGIN      
        1 ggcaatcagt aaaggaaata aatatgtcat cgaaaaaccg gacccgcaga accacaaccc
       61 gcaatatccg tttccccaat cacatgattg aacagatcaa catcgccctt cagcataaag
      121 ggtccggtaa cttttcagcg tgggttattg aagcctgcag gagaaggctg gcaacagatg
      181 caacgcatct gcgtccggcc agcatgacaa ataacgagaa atgaacgttc ggttacagga
      241 gcaggtaccc actgtcctcc aacaatattt catcttcata cccggcggaa caagacttac
      301 ccagccggga tgtacagaat aacaacagag tgataattaa tttctgatga aataatcagg
      361 gtgcagaagg actaaagata aacgttttct tcacgccttt acgcggcctg tccttctcaa
      421 atcgccattt tgccatcgcc tttacaacct gctcatcaaa cagatggtgc ggctctgaac
      481 ggataaactc aattcgggtg acagtaccat cagcaccaat atcaaacttc acatcaaccc
      541 gtccctttat ataatttgcc gctgcatagg ccggatattg tggtaatgcc ttaaccaact
      601 gtcggggcat atctgtttta tgttgcgtac agcccataac cagagaagac aacaaaataa
      661 ttaacggaag atttcttttc attttcattc ccggcacaga taagaataag tcttattcta
      721 acaatgccac cctgtcggtc atcaatcctc tgcttaatgg caacgacaat tatccgactt
      781 aaatcacaaa tcagacacat gacataacag a
//
LOCUS       DAZFFI010000447          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFFI010000447 DAZFFI010000000
VERSION     DAZFFI010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.451"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(12..>810)
                     /gene="mcrA"
                     /locus_tag="ACHJ12_005480"
     CDS             complement(12..>810)
                     /gene="mcrA"
                     /locus_tag="ACHJ12_005480"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methylcytosine-specific endonuclease McrA"
                     /protein_id="HFO4993077.1"
                     /translation="AECSIGEEDGVYGLILESWGPGDRNKDYNIALDYIIERLVDSGV
                     SQVVVYLASSSVRKHMHSLDERKIHPGEYFTLIGNSPRDIRLKMCGYQAYFSRTGRKE
                     IPSGNRTKRILINVPGIYSDSFWASIIRGELSELSQPTDDESLLNMRVSKLIKKTLSQ
                     PEGSRKPVEVERLQKVYVRDPMVKAWILQQSKGICENCGKNAPFYLNDGNPYLEVHHV
                     IPLSSGGADTTDNCVALCPNCHRELHYSKNAKELIEMLYVNINRLQK"
BASE COUNT          241 a          185 c          126 g          258 t
ORIGIN      
        1 aataaataat tttatttctg taatcggttt atattaacgt aaagcatctc gattagttct
       61 tttgcatttt tactatagtg caattctcta tggcaattcg gacaaagggc aacacagtta
      121 tctgttgtat cagcaccacc tgaagacagg ggaattacat gatgcacttc caaatatggg
      181 tttccatcat ttaaataaaa cggagcattt ttaccacagt tttcacatat acctttactt
      241 tgctgtaaaa tccaagcttt taccatcggg tctcggacat aaactttttg tagtctttct
      301 acctcaactg gtttcctaga gccctcgggt tgactcaacg ttttcttaat taatttacta
      361 accctcatat tcagaagcga ttcatcatct gtaggctgtg aaagctctga tagttctcca
      421 cgtattatag acgcccaaaa actgtcacta taaatacctg gaacatttat caatattcgt
      481 ttcgttctat tgccggaagg aatttccttt ctccccgtac gactaaaata agcctgataa
      541 ccacacatct tcaagcgtat atcgcggggg ctattaccaa tcaaagtaaa atattcacca
      601 ggatggattt ttctttcatc caaagaatgc atatgttttc tgactgatga tgacgccaga
      661 tatactacga cttgggatac accagaatca accaaccgtt caatgatata atcaagagcg
      721 atattgtaat ctttgtttct gtcacccggc ccccacgact caaggattag accataaaca
      781 ccatcctctt cacctatcga acactcagct
//
LOCUS       DAZFFI010000448          808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFFI010000448 DAZFFI010000000
VERSION     DAZFFI010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.452"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            56..331
                     /gene="yddL"
                     /locus_tag="ACHJ12_005481"
                     /pseudo
     CDS             56..331
                     /gene="yddL"
                     /locus_tag="ACHJ12_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            405..>808
                     /locus_tag="ACHJ12_005482"
     CDS             405..>808
                     /locus_tag="ACHJ12_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4993078.1"
                     /translation="MKTITLNDNHIAHLNAKNTTKLEYLNLSNNNLLPTNDIDQLISS
                     KHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTTQPPGLQNFTAIN
                     LDNNQLTHFDATNYDRLVKLSLNSNALESINFP"
BASE COUNT          287 a          159 c          137 g          225 t
ORIGIN      
        1 cgtttaaaat tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa
       61 attaaaaata gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc
      121 cgaagtctat aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg
      181 ttattttact gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa
      241 aggagaaacg caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa
      301 aggctataac gatgaagcca acggctcgcg cggcaagaat ctctgattga gttaagtgcc
      361 catcacaata tgatcctgac cttatattac acaatcatcc caggatgaaa acaatcactt
      421 taaacgacaa ccatattgca catttaaacg ccaaaaacac tacaaaactg gaatatttaa
      481 acttaagcaa taacaattta ctgccaacca atgacattga tcaactaata tcatcaaaac
      541 atctttggca tgtattagtt aacgtcatca acaatgatcc acttgcacaa atgcagtact
      601 ggactgcagt aagaaatata attgatgaca ctaatgaagt gaccattgat ttatcaggac
      661 ttaatttaac cactcaacca ccagggctgc aaaacttcac cgctatcaat cttgataata
      721 accaactcac acattttgat gcaaccaact acgatagact cgtaaagcta agtctgaata
      781 gtaatgctct tgagtcaata aattttcc
//
LOCUS       DAZFFI010000449          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFFI010000449 DAZFFI010000000
VERSION     DAZFFI010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.453"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(80..421)
                     /locus_tag="ACHJ12_005483"
     CDS             complement(80..421)
                     /locus_tag="ACHJ12_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO4993079.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
BASE COUNT          219 a          211 c          211 g          166 t
ORIGIN      
        1 gtgatgagat gcagcacact gagatccact tcacctgaag cagcccggaa ggccgtattc
       61 tgcagggccg tcatcgccat cagggacgct cccgccggta gtcaccggtc acggctgcgg
      121 aagaccgggg ttgcgcccgg gaaggggcca gataatggtc aataccgtca cgaatggtct
      181 gcagatctgc acgggcacgc agataatcaa aaaagtagcg ttccccctgt gaagaagcct
      241 gcagagaggc cgttctggcc cgctccagcg ctgcctgcac gtggtcaagc tggcgcatta
      301 cagtggccag ttcatccttt tcggcagcct gcgcaggtaa tgcaaaggac aaaagaaaca
      361 gcatccccgc ggcaccggcc agacggccgg cacggcaggg tgatgccgga aaagaaaaca
      421 tacgcacctc cgtcagagtt aaacagtgcg tacaggatgc gtaaggcaga ggactggatc
      481 agcgataaac tctatttgca aaacagaaaa tttttacggg agaatacact ggtgtggatg
      541 aaggcaaaca acccgggcaa tacgggcaat acgggcaata cgggcaatac gggcaacaat
      601 aaactatgtt ctgggggctg tcagcatagc cagtttctgc atctcgctgt tccagattct
      661 gttcaaataa atttattcca gcctgaatga tttattcctg tttattaaaa cacggcatca
      721 ctactgtttc ccgttaatga cacctcatta acaatttaaa gaggaaacac atcagcatgg
      781 aatttctccg tactgccctt ccgttct
//
LOCUS       DAZFFI010000450          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFFI010000450 DAZFFI010000000
VERSION     DAZFFI010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.454"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /locus_tag="ACHJ12_005484"
     CDS             <1..200
                     /locus_tag="ACHJ12_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO4993080.1"
                     /translation="NISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALISVA
                     TCWLLWKYRAILTNLVRLKIK"
     gene            216..797
                     /locus_tag="ACHJ12_005485"
     CDS             216..797
                     /locus_tag="ACHJ12_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO4993081.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          260 a          135 c          137 g          270 t
ORIGIN      
        1 aaaatatttc tgttgtattg tcaaaacgaa gagacattat tgagcaagca ccaacatatg
       61 aaattagcga acctgttttt gcttatgata aggctatgcc ggtttctatc ttacttgcat
      121 taatatccgt agcgacttgc tggctgttat ggaagtatag ggccattcta actaatttag
      181 tcaggttgaa aattaagtag acatatgaga gcgttatgaa tcttgcccta agaaagatta
      241 tatatgatcc aattagttat attcatcctc aacgagtaag cttgaataat acacctatta
      301 acaatccggt gcttagatct attaccaatg aaatgattgt tctgcagtac aatctttcag
      361 ttgaacattt taatctgaat agttcactta tttactacat aaacaattgg aatttatttc
      421 cattgttctg tttattcagt ggttatcact tttatcgaga aaggtttgca gaacgcggat
      481 ttttttataa ggttcctgct gtgctaaggg attatttatc agctataccg gtgaaaataa
      541 atgaaaaagc acgatacaaa cccggcatcg ctagctatca caatatcatc acatgtggtt
      601 tttcaacgtt gtcaccctat ataaggcaac aaccattggc aatgcagcaa cgatttaatt
      661 tattatttcc tgattttgtt gatcacatac aattgccgtt accacttgca tcaactcttt
      721 tagagagaat tacattttat gcgaaaaaaa atagagatga gcttgataaa atctcctgca
      781 aatggtgttg tgattaaaag aa
//
LOCUS       DAZFFI010000451          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFFI010000451 DAZFFI010000000
VERSION     DAZFFI010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.455"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>800)
                     /locus_tag="ACHJ12_005486"
     CDS             complement(<1..>800)
                     /locus_tag="ACHJ12_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO4993082.1"
                     /translation="CRPDGVYWVTPKVDKQSGEIIRPETWLCASVELLGAGVIGNEHY
                     RVMRWTDSTTNRLVTMAVPCCGIGDSDGWRLLKANGLKVTTNGKHRIPLADWMQLGGQ
                     HEEWHLSTKAGWHFGAYIMPDGTVIGESDKPVLFTGKSAAINGYSVAGTADSWRDSVA
                     RLAGGNPFMMLGIATSLSAPLVGLVGADGFGVHFFENSTAGKTTTQSVASSLWGDPEA
                     QRLTWYGTALGIANEAEAHNHGLLPLDEIGQAASARDVYVSAYTLFNG"
BASE COUNT          185 a          245 c          183 g          187 t
ORIGIN      
        1 aagccgttaa acagcgtata agccgatacg tacacatcac gggcgcttgc tgcctgtcct
       61 atctcatcca gtggtaaaag cccgtggttg tgtgcttcag cctcgttagc aatgcctaac
      121 gctgttccgt accaggttaa tcgctgcgct tcaggatcac cccataaact cgatgccacg
      181 ctttgtgttg tggtcttacc tgctgtggag ttttcgaaaa aatgcacacc aaagccatca
      241 gcaccgacca gcccgactaa tggcgcggat aatgacgtcg ctatgcccag catcatgaac
      301 ggattaccac cagccagccg cgccacgctg tcgcgccagc tatccgccgt acccgccacg
      361 gaataaccgt taatggcagc actttttccg gtaaacaaaa cgggcttgtc agattcgccg
      421 ataaccgtac cgtccggcat gatgtacgcg ccaaagtgcc agccagcttt cgtgcttaag
      481 tgccattcct catgctgtcc gcctaactgc atccagtcag ccagggggat cctgtgtttt
      541 ccgtttgtcg tgactttaag cccgttggct ttcagtaacc gccagccgtc actgtcgcct
      601 attccgcagc aaggcacggc catagtgaca agccgatttg ttgtcgaatc tgtccagcgc
      661 atcacgcggt aatgctcgtt accaatgaca ccagcgccca gtaattcaac cgaagcacat
      721 aaccacgttt caggccgtat aatttcgcct gattgcttat caacctttgg cgtaacccaa
      781 taaacgccat caggacggca
//
LOCUS       DAZFFI010000452          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFFI010000452 DAZFFI010000000
VERSION     DAZFFI010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.456"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>796
                     /locus_tag="ACHJ12_005487"
     CDS             <1..>796
                     /locus_tag="ACHJ12_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4993083.1"
                     /translation="MGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPR
                     GEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKA
                     ATPEAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWKTREF
                     VAWLKEKGAFDQPYWMMKASLPASMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRVT
                     IGEYGATGFINGTVCTCTVYGDTRYFHWRVDYSTKNKPDTVSQRDA"
BASE COUNT          217 a          176 c          276 g          127 t
ORIGIN      
        1 atatggggcc aaaaggcgag aaaggtgatc cgggaggtcc tgcaggcccg caaggtccta
       61 aaggtgaacg aggagaagcc ggaccacagg gaccgatggg agcacgaggt gagcgtgggg
      121 agactggccc ccgaggtgaa cctggtcctg caggtccgag aggcgaacga ggagagaccg
      181 gacctcaggg acctcgtgga gagccaggtc cggcaggcag cgctgcaaat gtggctgatg
      241 cgacgacggc acagaaggga attgtgcagt taagcagcgc aacggacagt gatgatgaaa
      301 cgaaggcagc caccccggaa gcggtgaaag cggcaatgga caaagcggac ggatgcctgg
      361 agaaagcgaa aaacggtgac gatatcccgg ataaggtgaa gtttctgaac accgtgggag
      421 cagccagagt atacgggcgg gacattcata cgggggccgg tgaatggaaa acacgcgagt
      481 ttgttgcctg gctgaaagaa aaaggggcat ttgaccagcc ttactggatg atgaaggcat
      541 cactgcctgc gtcaatgaat aaagtcatca cggatgtcgg accgggaaaa ctcaatctgg
      601 gtggctgtgt cattgaggtg atgggaacgt atgaggctgc catagtccgg gtcaccattg
      661 gcgaatacgg tgcaacgggg tttattaatg gcacggtctg tacctgtacg gtttacggcg
      721 acacacggta tttccactgg cgggtggatt acagcacaaa aaacaaaccg gatacggtca
      781 gccagcggga tgccag
//
LOCUS       DAZFFI010000453          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFFI010000453 DAZFFI010000000
VERSION     DAZFFI010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.457"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>793
                     /locus_tag="ACHJ12_005488"
     CDS             <1..>793
                     /locus_tag="ACHJ12_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4993084.1"
                     /translation="IEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDI
                     STKEGLYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGRE
                     KIENNYLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMA
                     TAVGFNALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNV
                     SIGADSRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDA"
BASE COUNT          230 a          144 c          241 g          178 t
ORIGIN      
        1 attgaaatag gtacaacgaa atttacagca aaagatgaat caaagggaat taatttcgac
       61 cataaatcac cggtggctga aggacgtaac tctatcgcga ttggtaccag tgcagaggct
      121 ggaggtgaca ttagcacgaa ggaaggtttg tacacaggcg agtctattgc gataggagcg
      181 aaaacgaaag caactaaaga acagtcaatt gcaataggtg gcgataccaa agctaccgga
      241 tggggggcta ttgttattgg tggggatgat ttaactcctt tgcatggtaa gacttatggt
      301 cgggaaaaaa tagagaataa ctatctggca tcggaagcga agggggatgg cagtattgcg
      361 ctgggcagca gaacttttgc taaaggcgaa ttgtcagtat cgcttggttc ggtgagttct
      421 gctgagggga tggcctcgaa tgcaattgga gccacggcgg aagctaaagg ggatatggcg
      481 actgcggttg gttttaatgc gctggctaat gccggtaatg ctctggccgt tggtagtcag
      541 tcctctgcaa ataaaaacga tgctcttgct gttggttcag aggcaaatgc cgctcagaat
      601 gaaacggttg ccctggggca tggagcaacc gttactgtcg caaaggggaa cgtgtccatc
      661 ggcgctgact ccagggataa gcaggcacaa aaagtaacag aggctaaggt cagaactaaa
      721 agaggcgcgg aagtcacata tagtggcttt gccggaaata acgcagcatc agtagtatct
      781 gttggtgatg cgg
//
LOCUS       DAZFFI010000454          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFFI010000454 DAZFFI010000000
VERSION     DAZFFI010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.458"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..550
                     /locus_tag="ACHJ12_005489"
     CDS             <1..550
                     /locus_tag="ACHJ12_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4993085.1"
                     /translation="SNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKG
                     DPGAQGPKGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVG
                     IRLGNKQTYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQ
                     WRTVSVGEYVSSGASGFEYFPL"
     gene            592..>786
                     /locus_tag="ACHJ12_005490"
     CDS             592..>786
                     /locus_tag="ACHJ12_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4993086.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPV"
BASE COUNT          211 a          164 c          265 g          146 t
ORIGIN      
        1 cagtaatgac gaaacaaagg cagccacgcc gaaggccgtg aaggcggcaa tggatgtggc
       61 aaatgaagcg aaaacaaagg cagaagaggc tgcagcagga ggtggtgttc ccggtccgaa
      121 aggagagaaa ggcgaccccg gagcacaggg tccgaaaggt gaaacgggag caacaggccc
      181 cgctggtccg cggggagcgc agggaccgaa aggggagaaa ggcgaccccg gtcccagagg
      241 tgagcgtgga gaaaccgggc cgatggggcc gcccggggca tcagacggga agagccgggt
      301 tgtgggtatc aggctgggta ataaacagac ttatgcgccg caaacagaca gcactacatg
      361 ggcagtggat ttggatattg gtgcaatgat tacggggatt ggcggttaca acgacggaaa
      421 caaaaccctt attgacaggg tatcttacag gccgcttcag gtgacatttg atggttctca
      481 gtggcgtaca gtcagcgtgg gtgagtatgt gtcgtcagga gcttcggggt ttgagtattt
      541 tccattataa agcgcatcat ctttcagaca tgtaaccggg aggacttaca gatgcacata
      601 aagaatttca gacaatacac gccggaaaat ccggatgtgc cgggtgcgat gtacctgaaa
      661 tcagaggatg gtcaggactg gtatgagtgt cagtcgttat tttcagcaga gacgctgaag
      721 gtggtttata acagtgccgg agtcattacc ggcatcagca gggtggcgtc agttctgtgg
      781 ccagtg
//
LOCUS       DAZFFI010000455          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFFI010000455 DAZFFI010000000
VERSION     DAZFFI010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.459"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..253
                     /locus_tag="ACHJ12_005491"
     CDS             <1..253
                     /locus_tag="ACHJ12_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4993087.1"
                     /translation="CHLMMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTN
                     SLLQNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            complement(336..599)
                     /locus_tag="ACHJ12_005492"
                     /pseudo
     CDS             complement(336..599)
                     /locus_tag="ACHJ12_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            679..785
                     /locus_tag="ACHJ12_005493"
                     /pseudo
     CDS             679..785
                     /locus_tag="ACHJ12_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible protein OsmY"
BASE COUNT          204 a          152 c          173 g          256 t
ORIGIN      
        1 gtgccatctt atgatgatgg caagagatat tactgcgtac cacctcaatt ctgtacacat
       61 cagtgcccgt cgtttccccg ggatttttca gtgggccacc ggtatgcaaa caaaaaatct
      121 gccgttaact aattctttgt tacagaatga ttacgcagaa agtatttttg tatttaatgg
      181 cgaacgtgac gtttcggaaa tgcctttacc ggaagaaatg ctactgactg gaaacgctga
      241 gtcagaaaga taattcggtg tattcatgac tgttatcaat gtttcatgtg tatgatgtca
      301 acgacggatg aaaagttatc cacttatatg aggcattata ggattatttc tggctgacgt
      361 tgtttcagtt cagaccaggg gcagtgaata tgctgacctg ctttttcgct gatatcagtc
      421 ctcctgtaat tgcatagata atagatatac ctctccgttg atcaggctgt ccaggtaatt
      481 gataccgcac ttcctcatca gttcgtagca gagtgtactt agcatggcta gtgcagtttc
      541 tgatgattta cagtgtgtat tcaggagagt aagaaattgc tcaaaacatc ctggtttata
      601 ctggtgcttt gatattcatt gtgtcccttt ttcgttcgtt attatttcca tatttaatat
      661 gatgcctgta catgtaaaat tggtgttcag gaggtctata atgaaatccg gcaggggact
      721 cccgtcagtc cggtcataat ggctggtgat gcctggctca cgacaaaaat tcgttctcag
      781 ttact
//
LOCUS       DAZFFI010000456          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFFI010000456 DAZFFI010000000
VERSION     DAZFFI010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.460"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..>780
                     /locus_tag="ACHJ12_005494"
     CDS             98..>780
                     /locus_tag="ACHJ12_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4993088.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDV"
BASE COUNT          256 a          134 c          171 g          219 t
ORIGIN      
        1 atagacgaga tttttcctgt tatgatataa tatgccgaat taacacatgt taacgattta
       61 ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat gggccaatat
      121 tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa aattcggtgt
      181 agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca catctttaaa
      241 attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg ataaagcaat
      301 tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggacgttatg aaggctttgg
      361 tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc gtacgattgc
      421 taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag gttctgtcag
      481 ctatatgttt gacaatacgg gtgtgattgt atttaaagga tcagaccctg accatatttt
      541 tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag aaggtaacat
      601 tgttatttat accgaaccta ctgaccttca taaaggaatc gcggctctaa aagcagctgg
      661 aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag ttgagctttc
      721 cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg acgacgatgt
//
LOCUS       DAZFFI010000457          774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFFI010000457 DAZFFI010000000
VERSION     DAZFFI010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.461"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..603
                     /gene="yfdR"
                     /locus_tag="ACHJ12_005495"
     CDS             67..603
                     /gene="yfdR"
                     /locus_tag="ACHJ12_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HFO4993089.1"
                     /translation="MSFIKTFSGKHFYYGRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPQAMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFEVIPLAPGHA
                     YEIFMERFKELHKIHKQS"
     gene            659..>774
                     /locus_tag="ACHJ12_005496"
     CDS             659..>774
                     /locus_tag="ACHJ12_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4993090.1"
                     /translation="MLQMLTLEEWAAEKFRSNPPSVSTLRRYAKQHLFCPPA"
BASE COUNT          217 a          170 c          178 g          209 t
ORIGIN      
        1 ccggatggtg agggcttcct tttaccagaa ttcagcgcgg tgcagtgcat atacgtggag
       61 aacaaaatgt catttattaa aactttttcc gggaagcatt tttattatgg caggataaat
      121 aaagacgaca tcgatattaa cgatatcgcg gtttcccttt caaatatctg tcgctttgcc
      181 ggtcatcttt cgcacttcta cagcgtcgcc caacatgcgg ttctttgcag ccagctggtg
      241 ccgcaggaat ttgcttttga agcgttaatg catgatgcaa cagaagcgta ttgccaggac
      301 attcccgcac cactgaaacg ccttcttcct gactataaac ggatggaaga aaaaatagac
      361 gccgtaatcc gtgagaaata cgggttacct caggctatga gcacgcccgt gaaatatgcc
      421 gatctcatca tgctggcaac cgaacgccgc gatctcgggc ttgatgatgg ctctttctgg
      481 ccagtattgg aaggtatccc agcaacagag atgttcgaag ttattcctct ggctcctggc
      541 catgcctacg agatatttat ggaacgtttt aaagagctgc ataagataca taagcaatcc
      601 tgacagcgaa attaactagt gaaatagttt tgtagcaaaa gaaatgaggt tatcaaaaat
      661 gcttcaaatg ctgacacttg aggaatgggc tgcggaaaaa tttaggagta atcctccaag
      721 tgtgtccaca ttgcgtcgtt atgctaagca gcatttattt tgtccaccgg caat
//
LOCUS       DAZFFI010000458          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFFI010000458 DAZFFI010000000
VERSION     DAZFFI010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.462"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>763
                     /gene="ehxD"
                     /locus_tag="ACHJ12_005497"
     CDS             <1..>763
                     /gene="ehxD"
                     /locus_tag="ACHJ12_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4993091.1"
                     /translation="LARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQ
                     AKNEHAVWLAQVSQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKN
                     RQRKASSFIKAPVSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGF
                     IQPGQEVVIKVDAYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGER
                     KKIPVTAGMTVMAEIKTGVRSVISYLLSPLKETINE"
BASE COUNT          271 a          116 c          165 g          211 t
ORIGIN      
        1 cttgctcgtg ttagcctgta tgagcatcag gtatcacagg aaggaagaaa acttaatgat
       61 tttaagtatt tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat
      121 agctatattc aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa
      181 aaagaaatag aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa
      241 attatcgaga agcacagaaa atcaacagat aacattgtgt tactggagca tgagcttgaa
      301 aaaaacaggc agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag
      361 gagttaaata tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt
      421 gtccctgata atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt
      481 atacaacctg gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt
      541 taccttacag ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacagga
      601 cttgtattta acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa
      661 attcctgtta cagctggaat gactgttatg gcagaaataa aaacaggagt gcgtagtgtt
      721 atcagctatc ttcttagccc attaaaggaa actattaatg aaa
//
LOCUS       DAZFFI010000459          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFFI010000459 DAZFFI010000000
VERSION     DAZFFI010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.463"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(265..>754)
                     /locus_tag="ACHJ12_005498"
     CDS             complement(265..>754)
                     /locus_tag="ACHJ12_005498"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFO4993092.1"
                     /translation="NAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALP
                     DSLTNPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPS
                     CPLCNGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDK
                     LN"
BASE COUNT          172 a          225 c          167 g          190 t
ORIGIN      
        1 ttatgtgccc ggacagactg aaatcactga tggaaaaagt caacagctaa ccaaaaacaa
       61 aaagcaccgg caagccggtg cttcaggggg ataccaaatc ccggtgttga ttctcatctt
      121 ctccggagag agcgactccc ggcacggttt tcacggagtg catgtgcctt gtaaccccgc
      181 cgtgtgcata acgggttacc ggacctctca gggctgtcag ccccactgtg gtcaacaggg
      241 tgataccaga actcgcccgc aattttaatt cagtttgtct ccgacatcac cagatttttg
      301 tttatgagaa aaattaattt ttcttttcct gcccctgctg tttccctgct caccagcaat
      361 tcccctgcag tcgggataac gggagcatcc ccagaaaaaa ccgctttttc ccttccgttt
      421 caccgtcgga ccgttacaga gtggacatga aggacctgat tccactgaca gattaaccgt
      481 ctggttacgt ccggataaca ccaggtgcct gatccactga tgctgtttct ccataaacgc
      541 ctccagtgag attttccccc gggcaacatc atccagcgcc tgttcccata aagccgtcat
      601 acccggattt gtcagagagt ccggcaatgc cgccaccagt tctcttccca gttgcgtgga
      661 acggatatgt tttcccgctc tctcaatata gttgcgcgca aataacgttt ccaggatccc
      721 cgcgcgtgtg gcttccgttc ccagtcccgc attc
//
LOCUS       DAZFFI010000460          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFFI010000460 DAZFFI010000000
VERSION     DAZFFI010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.464"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            459..>746
                     /locus_tag="ACHJ12_005499"
     CDS             459..>746
                     /locus_tag="ACHJ12_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible protein OsmY"
                     /protein_id="HFO4993093.1"
                     /translation="MRLLYSAIVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQ"
BASE COUNT          228 a          147 c          181 g          190 t
ORIGIN      
        1 aggctgaagc ttgaaaaact tgagatcaaa agagaaatcc agaaaatcct tgaagatgag
       61 tcagaaaagg gcgaacggta atcataaacg gtatattcaa agacttgttt tctctgtttt
      121 atgaaaaagg caccgggggc tccacggtgc cttttagcct gtgtataacg aaaaggagat
      181 cctgttcagc gaaccagata gttaaaaaaa gaatctttac gacggtatca aagttttcgt
      241 gcaaaaaaca tgatcaatat taaattccag tacatccata tccgatataa atctttccgt
      301 ctgacagata cagatatgcc acgtaacgca tccgctgttt ccggctgtgc cggaggataa
      361 taacagtctg tagcttgttt tgtttcactg aaggtaaact ttttgtacga taaattccgg
      421 gaatatgaag taaaaatcaa aacaattccg ggggagatat gcgtttatta tattcagcta
      481 ttgtcctgat ttcagcctta ttgttacagg gatgcgttgc tgcaactgtg gtgggtacag
      541 ccgctgtcgg gacaaaggca gcaaccgacc cacgcagtgt cggaacgcag gtggatgatg
      601 aagtgctgga gctgagggtc agcagtgcac tttcaaaaga tgaacaaatc agaaaagaag
      661 cacgcatcag tgagattgca tatcagggac gcgtgctgct gatcggtcag tctccggacc
      721 gtgaattatc tgcccgtgcc cgtcag
//
LOCUS       DAZFFI010000461          737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFFI010000461 DAZFFI010000000
VERSION     DAZFFI010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.465"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..255)
                     /locus_tag="ACHJ12_005500"
     CDS             complement(<1..255)
                     /locus_tag="ACHJ12_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO4993094.1"
                     /translation="MKRILSFCFLFSVHNVFASPLPALLRDALIHDPSVMEARAEATS
                     ARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYDSEDILP"
BASE COUNT          254 a          151 c          164 g          168 t
ORIGIN      
        1 gggcaaaata tcttcagagt cgtagtcata gtcgtagcgg tgactctggg aaaggagttt
       61 actgcctgtt gctgtgacga ccggccagtg tgctgaacga gcctgctcca cacgggaacg
      121 ggcagaggtg gcttctgccc ttgcctccat cactgatgga tcatgaatga gagcatccct
      181 caggagggcc ggcagtggag aagcgaacac gttgtggaca gagaaaagaa aacagaaaga
      241 taatattctc ttcacccgaa cctccgggac acgcgggaaa tataatcagg catcatgatg
      301 caaccgggta aaatatcgag taaaattgaa atgcagagac accggaattg atccaagcta
      361 ccgccattat cttccgcgct gacagaagat aataaaaatg tgagatactt caattttatt
      421 gaaaagctga aaaaccctta caatccttgc agtttatgga gaaaattttc acaaggtaaa
      481 ttttaaaaca aacaaattta atgtaatata attgggggag gattaaccat cgatgattgc
      541 cggaacaaaa aacaatataa accaacaaga tagaaaatca tcacaacaaa gggaaaggtc
      601 tgtggataag agggggagga actttctgct tcatgtcaca taattgatat actttatccc
      661 gcaacctcca gttgcattat atataattca acctcaggct cccattacag gcaaaaaata
      721 atatatcgca agccgca
//
LOCUS       DAZFFI010000462          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFFI010000462 DAZFFI010000000
VERSION     DAZFFI010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.466"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>728
                     /locus_tag="ACHJ12_005501"
     CDS             <1..>728
                     /locus_tag="ACHJ12_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4993095.1"
                     /translation="TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAV
                     HREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAWG
                     KAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRYHY
                     DLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDGHGWLREISHLSEGHR
                     VAVHYGYDDKGRLTGERQTVENPET"
BASE COUNT          170 a          217 c          239 g          102 t
ORIGIN      
        1 acgacaacag atgcgacggg cagcacccgg cagatgacct ggagccgcta cgggcagttg
       61 ctggcgttca ccgactgctc gggctaccag acccgttatg aatacgaccg cttcggccag
      121 atgacggcgg tccaccgcga ggaaggcatc agcctttacc gccgctatga caaccgtggc
      181 cggttaacct cggtgaaaga cgcacagggc cgtgaaacgc ggtatgaata caacgccgca
      241 ggcgacctga ctgccgttat caccccggac ggcaaccgga gcgagacaca gtacgatgcg
      301 tggggaaagg cggtcagcac cacgcagggc gggctgacgc gcagtatgga gtacgatgct
      361 gccggacgtg tcatcagcct gaccaacgag aacggcagcc acagcgtctt cagttacgat
      421 gcgctggacc ggctggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
      481 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggcactac
      541 gatgcgtcgg accgcatcac gcaccggacg gtgaacggcg acccggcaga gcagtggcag
      601 tatgacggcc acggctggct gagagaaatc agccacctga gcgaaggcca ccgtgtcgct
      661 gtccactacg gctacgacga taagggccgc ctgacgggcg aacggcagac ggtggagaac
      721 ccggagac
//
LOCUS       DAZFFI010000463          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFFI010000463 DAZFFI010000000
VERSION     DAZFFI010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.467"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..645
                     /locus_tag="ACHJ12_005502"
     CDS             154..645
                     /locus_tag="ACHJ12_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4993096.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAHIELLNYNSE
                     DNG"
BASE COUNT          284 a          113 c          115 g          216 t
ORIGIN      
        1 agtttgtagt gaacagtact tttaccaata atgaaaaata taccataagc aatgcaagct
       61 taaaactcat aatttaaaaa agataaatat aaaaatatca atgagtcaat caataataat
      121 ttggcatcac gagacacatc acagaggaat attatgagca cagaaacaat tgaaatattc
      181 aataatagtg atgaatgggc aaatcaacta aaacacgcat tatcgaaagg agaaaatctg
      241 gcattactac atggtttaac tcctgatatc cttgatagaa tatatgcata tgcattcgac
      301 taccatgaaa agggtaatat aacggacgca gaaatttatt ataaattttt gtgcatttat
      361 gcgttcgaaa atcatgagta tctaaaagat tttgcatcag tatgtcagcc caaaaagaaa
      421 tatcaacaag catatgacct ttacaaacta agttacaatt actccccgta tgatgactat
      481 tcagttattt atcgtatggg tcaatgtcag attggggcta aaaatatcga taacgcaatg
      541 caatgtttct atcacattat taacaattgt gaggatgata gtgttaagag taaagcgcag
      601 gcacatattg aactcttaaa ctataattca gaagataatg gctaagcatt acaatcattt
      661 ggttaaattt gctgattata gtgcagttaa ccactataac cagcacctct tatgttttct
      721 taaatcag
//
LOCUS       DAZFFI010000464          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFFI010000464 DAZFFI010000000
VERSION     DAZFFI010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.468"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            60..>726
                     /locus_tag="ACHJ12_005503"
     CDS             60..>726
                     /locus_tag="ACHJ12_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993097.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQN"
BASE COUNT          289 a          142 c          107 g          188 t
ORIGIN      
        1 tgttctaatt atccaattta aacatcttag cataaaacaa atgatgaata aggaatttta
       61 tgtcaattga ctttacccca ggtataataa atacatatca cggcgatatt tataactgca
      121 caacaaatac cgataatgct aaaacgccag acacaccgaa gtggccttgc gataattggg
      181 aagaacaaca acccattaat tccacattct ctggagaagg atatatctct gatcaatatg
      241 atttagcgca acaccaactt caacaaatta atgcgtgcca cacgaacact acatatacaa
      301 atgcagacta ctcaaaagtt gtggctcaac ttgtaagtct tattactaac attgaaacga
      361 taagttcgac acaacttacg caacagacac aatcaatact taatcagatt aataacatta
      421 gatatgaaaa aaacaaaagt gctgaatgtc gaatcattgt tgtcgctaat cctaaacccg
      481 acaaggcaat cataactaaa attagcgtag aagagggaat acccataaca ttcagtgtcc
      541 aaaccatgtt ttcagatact aattttattg ctgaacaacg agctgactta ccaactaata
      601 taaaagatat tcaaagcctg tatcaaaaaa tgacgaagtt atatattgag cacagtgaaa
      661 ataaaaacag gatgaaagtc tttgccggaa ccaactttat tgatttcaat atgacaggac
      721 agaacc
//
LOCUS       DAZFFI010000465          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFFI010000465 DAZFFI010000000
VERSION     DAZFFI010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.469"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..142
                     /locus_tag="ACHJ12_005504"
     CDS             <1..142
                     /locus_tag="ACHJ12_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993098.1"
                     /translation="AHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLYQL
                     QA"
     gene            146..>717
                     /gene="ehxD"
                     /locus_tag="ACHJ12_005505"
     CDS             146..>717
                     /gene="ehxD"
                     /locus_tag="ACHJ12_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4993099.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSS"
BASE COUNT          238 a          105 c          147 g          227 t
ORIGIN      
        1 tgcacacagg ttgtcaactg taaaaaatgc aaacagaata attgttatgg ataatggatt
       61 tatttctgaa gatggcacac ataaagagct tatctccaaa aaagacagtt tatatgcata
      121 tttatatcag ttgcaggcat aacgtatgcg cttttacatg aaaggtttat gggatttgat
      181 ttgccgttat aagacggttt tttctgatgt ctggaaaatt cgtcacacac ttgatgctcc
      241 tgtgagagaa aaagatgagt atgcatttct tcctgctcac cttgaactca ttgaaacacc
      301 agtatccaga cgttctcatt ttgttatatg gagtatttta ttatttgtaa ttatatctct
      361 tctcttatct gttcttggga aagttgaagt ggtctcagta gcaaatggta agtttactca
      421 tagtggaaga agcaaagaaa taaaaccgat agagaatgcg attgtagaaa aaataatggt
      481 gaaagatggc gcttttgtaa aaaaaaatga tccattagtt gaattgacgg tgcctggtgt
      541 tgaatctgat attttaaaat cagaagcatc tttgttgtat gaaaaaacag aacaatatcg
      601 ctacgcaatc ctttctgaat caatacaacg gaatgagctt cctgaaataa ggataacaga
      661 ttttcctggc ggagaagata atgcaggagg tgaacatttt cagagggtta gctcatt
//
LOCUS       DAZFFI010000466          714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFFI010000466 DAZFFI010000000
VERSION     DAZFFI010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.470"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            93..488
                     /locus_tag="ACHJ12_005506"
     CDS             93..488
                     /locus_tag="ACHJ12_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993100.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            485..>714
                     /locus_tag="ACHJ12_005507"
     CDS             485..>714
                     /locus_tag="ACHJ12_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993101.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVV"
BASE COUNT          150 a          181 c          194 g          189 t
ORIGIN      
        1 taatttttat catccggttt tgaatttatt gctgattgtc cctttgcctt tgcgcacgct
       61 gtcagatacc agttgtctga cggagtcgca tcatgtaccg actgttactg tattccttcc
      121 tgatactacc ggtggctgca tctgccggta caacaattta taccgattcg caccagcgcc
      181 ccatgaaccc gccagcaggt gttcgtgttg tcctgcttga tgctcctgag cagacgcagg
      241 atacattttt cggggtactt cccgcagagc ctgcagaagc atctgcccgt gtcatggaac
      301 ggatgcagtc gcctgaatgg cagtcgtttc aggcagggct tgctgaacac taccgtgcat
      361 tagcacatgc ctggtccctt ggactgaaaa aatatccggc tgtggttttt gatgacagtg
      421 aagtggtcta cggaaccaca gacgtggtgc tggcagaaca actgcgtaca gggggagggc
      481 agccatgatt tcctgcacat cacgtcatcg cgccggtgtt ctgctgttaa gtatggcggt
      541 attgccggtg gcagaggcat cgctgaacac cgcgcagata gtggccagtg cagcctcaca
      601 gagctgtatc agctggcggg tcagcgggat ttgctactgg ctgttctgca ccaaattcag
      661 ttgcaaggtc agaacatcag tgaaagtttc ccattatatc ccggaagtgg ttgt
//
LOCUS       DAZFFI010000467          711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFFI010000467 DAZFFI010000000
VERSION     DAZFFI010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.471"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..309
                     /locus_tag="ACHJ12_005508"
     CDS             <1..309
                     /locus_tag="ACHJ12_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4993102.1"
                     /translation="AGFKGMIPPKITSRPQCCDDDAMVICGCIARLYRNNRDLHDLLV
                     DYYVLGKTFMALARKHGCSDGHIGKRLQKAEGVVEGMLMMLGVRLEMDRYVERDSVTA
                     "
BASE COUNT          206 a          146 c          202 g          157 t
ORIGIN      
        1 gccggattta aggggatgat tccgccaaag ataacatctc gtccacagtg ctgtgacgat
       61 gatgcgatgg tgatatgcgg gtgcatagcc cgcctttacc ggaacaatcg cgatctgcat
      121 gacttgctgg ttgattacta cgtgctgggg aaaacgttta tggcgctggc acgtaagcat
      181 gggtgctctg atggacatat cggaaagcgg ttacagaaag cggaaggtgt tgttgaaggc
      241 atgctgatga tgctgggagt gaggcttgag atggatcggt atgttgagcg cgattcagtg
      301 acggcataac gcgcagaaaa aaccgcagct atgagaactg cggtggagaa gtaagacttg
      361 ccagtcaatc atgaaaaacg taatcgcctg ataattatgc cacgagacaa agggcggtgc
      421 accctgaatg gtgataatca ccgcgcagac catccagaca accataacga acatcgttac
      481 agttggagct tctaccacca ttggtggaaa aggtgcacat ctgaatcatc attatggtga
      541 tcaagggcat gtaaagccca ccagcctgat taaaggttaa ccggaaaaag ccaggtatgg
      601 agggagtggt tagccacgaa caagccggac accaccagca gaaggtatta tgattttcgt
      661 aataccttta gcaaagcgtg aatcatcatt acggtaggga tggtcatact g
//
LOCUS       DAZFFI010000468          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFFI010000468 DAZFFI010000000
VERSION     DAZFFI010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.472"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>705
                     /locus_tag="ACHJ12_005509"
     CDS             14..>705
                     /locus_tag="ACHJ12_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993103.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQV"
BASE COUNT          182 a          151 c          246 g          126 t
ORIGIN      
        1 ggggaggtga agcatgcaga ttaaatccaa tacgatcgtg aagtatgtgg tgccctgtgt
       61 ggtggtggcg gggatcctga cgggattaaa aagctgtgga ggagataccg gggagccctc
      121 gtctgcggaa agctcaggaa cgctggccgg actgtcaccg gatgaactga aggccctggg
      181 cgtggaagga gacacacctg aagataccct gcgcacactg attgggcgta tgaatcaggt
      241 gagggagcgg caactggtac tggacaaaca gaatgaaaag ttgctgaaag aaaatgaaat
      301 gctgcgcaag cggggcacgg atgtttccgg tcaggtcagt gaggcagtgg caggattgca
      361 gaaaaattat gagcagtcgc agcgccagtt ccggaatgaa cagaacgcgt taatgacaca
      421 ggttcagtca ctgacagagc aactgaagcg tcagcaggag agtgctgacg ccggcgccgg
      481 aggacaggag agcgatattc cagcaggtct gggccttgac ggactgggga gcgtgacagg
      541 atcggcagcc tcaccgggaa cggatggtct gatgtgggtg acaccggccg atcaggtgca
      601 ggatgaagcg tcgtcggtgg gggataagag cggaccgcgt tttccagtct cttttctgga
      661 tgacaatcca ctgacccgtc agaaagcggc ttatgaacag caggt
//
LOCUS       DAZFFI010000469          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFFI010000469 DAZFFI010000000
VERSION     DAZFFI010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.473"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..620
                     /gene="pinE"
                     /locus_tag="ACHJ12_005510"
     CDS             66..620
                     /gene="pinE"
                     /locus_tag="ACHJ12_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO4993104.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWEQAGRLIASGVP
                     RQKVAIIYDVGVSTLYKRFPAGDK"
BASE COUNT          207 a          132 c          191 g          174 t
ORIGIN      
        1 gtttatgaaa atgcagaaat aacgagcaaa tggcatcatt cctgcttttg tcagggagat
       61 ctaccatgct tattggctat gtacgtgtgt caacaaatga ccagaacaca gatctacaac
      121 gtaatgcgct gaactgtgca ggatgcgagc tgatttttga agacaagata agcggtacaa
      181 agtccgaaag gccgggactg aaaaaactgc tcaggacatt atcggcaggt gatacgctgg
      241 tggtctggaa actggaccga ctggggcgca gtatgcggca tcttgtcgtg ctggtggagg
      301 agttgcgcga acgaggcatc aactttcgta gtctgacgga ttcaattgat accagtaccc
      361 caatggggcg ctttttcttt catgtgatgg gtgccctggc tgaaatggag cgtgaactga
      421 ttgttgaaag aacaaaagct ggactggaag ctgctcgcgc acagggacga attggtggac
      481 gtcgtcccaa acttacacca gaacaatggg aacaggccgg acgattaatt gcatcaggag
      541 ttcctcgcca gaaggtggcg attatctatg atgttggtgt gtcaactttg tataagaggt
      601 ttcctgcagg ggataaataa agttaaagac actttgtgta caaaagaaag taaaacaaca
      661 gcaacttgtt gcaattttat caataaaagt agtattgtcg tgaa
//
LOCUS       DAZFFI010000470          703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFFI010000470 DAZFFI010000000
VERSION     DAZFFI010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.474"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(204..>703)
                     /locus_tag="ACHJ12_005511"
     CDS             complement(204..>703)
                     /locus_tag="ACHJ12_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO4993105.1"
                     /translation="PSVTVTEWIATAYQWLFVETGFLSWLKQQNAGAVVGNSVSQYSE
                     DVGRWLSHYLCEYLMATVYVTVVTLIRVTILVLSVPLFVMVILIAVVEGLGRRDLRRY
                     GAAYESSFVYHHAKKSIKPAIYIPCIFYLSWPTSVYPNLLIWPAAMLLGVAITVTMAS
                     FKKYL"
BASE COUNT          176 a          213 c          155 g          159 t
ORIGIN      
        1 catttatgaa gaacctcgtt gccccccgtg gatctatgac gtcatcaggg ccatcactct
       61 gttcattatt ttcgtcaaca ttgttgtcat tccggcgctg tcgttactct gacactgacg
      121 cgcactgacc gtaagggcgt ggcttgtctg ccgacaccct gccacaaaac aagttttgca
      181 tcagggtgtc cggcctctgt ggattataaa tatttcttga aggatgccat ggtcacggtg
      241 atggccaccc ccagcagcat ggccgccggc catatcagca ggttcggata caccgacgtc
      301 ggccaggaca ggtaaaaaat gcagggaata tatatcgccg gtttaataga cttttttgca
      361 tggtgataaa caaagctgga ctcatacgca gcaccatacc ggcgcagatc acgacgcccc
      421 agtccctcca caaccgcgat cagtatcacc atcacaaaca gcggaacaga caacaccagg
      481 atcgtcaccc gtatcagtgt gaccaccgtg acatatactg tggccatcag gtactcacac
      541 agataatggc tcagccagcg ccccacatcc tcactgtact gtgacacact gttacccacc
      601 acggcaccgg cattttgctg cttcagccac gacagaaaac ctgtttccac aaacagccac
      661 tgatacgccg ttgctatcca ttctgtcacc gttaccgatg gtg
//
LOCUS       DAZFFI010000471          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFFI010000471 DAZFFI010000000
VERSION     DAZFFI010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.475"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>698
                     /locus_tag="ACHJ12_005512"
     CDS             <1..>698
                     /locus_tag="ACHJ12_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO4993106.1"
                     /translation="LSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGC
                     GGDDEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGEL
                     EDLYWFQVDMPERMHSDRLRRDYRNFEEYQYIAKSTKDVEMNQSLRLLELCFPLPKKL
                     ELLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLL
                     YVAVTRALRTLVLN"
BASE COUNT          165 a          138 c          226 g          169 t
ORIGIN      
        1 ctttcccatc ctgcactgaa gaatgcggac cggttgtggc tgacacagag tttccgtttt
       61 ggtccggcgg tggctcgtat ggcaaatctg cttctgcagc gtgaaggtga aacacgagag
      121 gtgaaaggtt gtggtggtga tgatgaggta ttgctgaaat gccatgcgcg tgaacatctg
      181 caggggcatt acacggtact cagtcgtact gtcgcgggtg tgattggtac tgcgctgatg
      241 gcggcaatga aagggcaaaa ggtttactgg gtggggggaa tcgaaggtta caggaccggg
      301 gaacttgagg atctctactg gttccaggtt gatatgccgg aacggatgca ttcagacagg
      361 ctgcgccggg actacaggaa ttttgaagaa tatcagtata ttgcgaaatc cacgaaggat
      421 gtggagatga atcagtccct tcggcttctg gagttgtgtt ttccgttacc gaaaaaactg
      481 gaacttctcc ggcagtatac ggtaacgaat gagcaggatg cagatatcac ggtgtcaacg
      541 gcacaccgga gcaaggggct ggagtggccg gttgtaatgc tggatgagga ttttcaggat
      601 atcacggatc cgctgatgac ggaggctgag cgtcatgatg aaacctgtct gttatatgtg
      661 gctgttaccc gggccctcag gacgctggta ctgaatga
//
LOCUS       DAZFFI010000472          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFFI010000472 DAZFFI010000000
VERSION     DAZFFI010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.476"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..505)
                     /locus_tag="ACHJ12_005513"
                     /pseudo
     CDS             complement(44..505)
                     /locus_tag="ACHJ12_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
BASE COUNT          209 a          171 c          127 g          186 t
ORIGIN      
        1 aatatcgcat ttcaatgaga actcaatagc cacagaaata tcattatttc cgtgagcgcc
       61 atacccaggg cggaacaggc gcgctgtctg cccacagacc acgtttctgc tgtcgtgctt
      121 cttcctgcag gacgggaaga acaggatcgg tgttgtactg ctcataaacc catgcagcgc
      181 ctgcacggac cataaactga ttaacattca ttccgtctgg tgcataaacc tgcccgagaa
      241 tacgaccata acgatctcgc tgaaaatatg tgacagtaac cgtttttccc gcaaccagag
      301 attacattat tttttcagtc caccgtccat agtcctgttt cttttcaggt gcatcaatat
      361 taacaagacg gacacgcacc ggatgctgag ataacatgac gtcaatggta tcgccatcca
      421 gaacacgcac gacccgtcca tgaatatcag cagaaaaaac ctgatatgaa aaaacaaaca
      481 ataccactgg tatatatttc ttcattttac tctccgtcac tgattacttt ttgtgattgc
      541 aggaattcca ttccgtgatg aataatgcct cactcatcac gccaaaaata aaccgttaaa
      601 ttgtggtcta ttcagtaaaa taagattcag tggctgaata cacatatagc catgtgttac
      661 taaggtctca tgaattaatc atgcattctg tac
//
LOCUS       DAZFFI010000473          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFFI010000473 DAZFFI010000000
VERSION     DAZFFI010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.477"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>688
                     /locus_tag="ACHJ12_005514"
     CDS             <1..>688
                     /locus_tag="ACHJ12_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFO4993107.1"
                     /translation="IPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDIAGKKADLI
                     ISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLPFILLFIGV
                     IGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVEALDGIETL
                     KLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLVILGTYLIH
                     SDDPHNRITMG"
BASE COUNT          152 a          159 c          193 g          184 t
ORIGIN      
        1 atacccaatc gctcctggga aacgttatgg atgctgagta tcggggtgat tgcagccatt
       61 ctgtttgagt gtgctgcaaa gttaatccgg agctatctga cggatattgc gggtaagaag
      121 gcagacctga ttatcagctc agcactgtta aggcgggtga tggcagtgaa aatgtcggac
      181 aggccggcgt cttccggttc atacgccagc aatttgcggg aatatgagtc tgtcagggag
      241 tttatgacca gcgccacgct gctggctttt gtggatttgc cgtttatcct gcttttcatc
      301 ggtgtcatcg ggcttgtggg gggctggctt gctgttgttc ctcttacact ggttccgctg
      361 gtgattctgg caggaatatt actgcagcga ccgctttcgc gtcatattaa tgcctccatg
      421 cgggaatcat cgcagcgcca gggattactt gtggaggcac tggacggaat agaaaccctg
      481 aagctgaata atgcagttaa ctgggtccag cgccgctggg atggatatac agccagtgtc
      541 gcccgctcgt ccatacgggt aaaaaatacc agtaatgtca taatctgctt ttccacagca
      601 gttcagcaac tgaatactgt tctgctggtc attctgggga cgtaccttat tcacagtgat
      661 gacccacata accgaatcac catggggg
//
LOCUS       DAZFFI010000474          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFFI010000474 DAZFFI010000000
VERSION     DAZFFI010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.478"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..665
                     /locus_tag="ACHJ12_005515"
     CDS             123..665
                     /locus_tag="ACHJ12_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4993108.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT          204 a          125 c          151 g          204 t
ORIGIN      
        1 aaagatttgt cacttattgg atttagtatg cttgatttaa atggcggtca attattgtta
       61 ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc aggatgatga
      121 cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg tccagcgtta
      181 tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt aaagttattg
      241 ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg ccaaatgtca
      301 gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat gtaccaattg
      361 ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt agtggtacgg
      421 ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca gcgacgaatg
      481 ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt accgaaacgt
      541 cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt gattatatcg
      601 ctacggggaa agcgacttct ggtaatgtga atgcggtaac aaatttccat attaactatt
      661 attaatagaa ctcattaatt gttt
//
LOCUS       DAZFFI010000475          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFFI010000475 DAZFFI010000000
VERSION     DAZFFI010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.479"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..354)
                     /locus_tag="ACHJ12_005516"
     CDS             complement(<1..354)
                     /locus_tag="ACHJ12_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4993109.1"
                     /translation="MVAVNHTPHLVHTQTAFVWRFLALNIGEKNQLIASVENSYDSRA
                     PHKTGAGIGVLGKTEAHNTRPACFFVPHSHTLSMVGCTGAEQSAPVSSVSGYANPVQS
                     ATSEIGVSGGGYLNHT"
     gene            complement(380..>684)
                     /locus_tag="ACHJ12_005517"
     CDS             complement(380..>684)
                     /locus_tag="ACHJ12_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993110.1"
                     /translation="CYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFSSGEIR
                     ILNCGDCPSPLGLLSRLIVDKWSPIELAFLLLGGHLNTRALKKAKPRKCHYHSQGF"
BASE COUNT          191 a          162 c          181 g          150 t
ORIGIN      
        1 agtgtggtta agataaccgc caccggaaac gccaatttcg ctggtggcgg actgtacagg
       61 gttggcgtaa ccggacactg aagaaaccgg cgcggattgc tccgcccccg tacagcccac
      121 cattgataag gtgtgactat gcggcacaaa aaaacacgcg gggcgcgtgt tatgcgcttc
      181 agttttgccc aggacgccaa tcccggcacc cgttttatga ggtgcgcggg aatcatagct
      241 attttcgaca ctggcgatca actggttttt ttctccgata ttcagggcca gaaaacgcca
      301 cacaaaggca gtttgtgtgt gtacaaggtg tggggtatga tttacggcaa ccataacggc
      361 tcctagttta cgttgttggt tagaagccct gcgagtggta atgacacttg cggggctttg
      421 cttttttaag tgctcttgtg ttaaggtgac cacctaacaa tagaaaggct aattcaatag
      481 gtgaccactt gtcaacaatc aaacgcgata aaagccctaa aggggacggg caatccccac
      541 aattcagaat gcgaatttct ccagagctaa aagagcaatt cgacaaagaa gcacagagcg
      601 acggcataag cctagccaac tggcttaaag agttggggcg cactgaacta aagcggcgtg
      661 gcatagaacc caaaggataa caac
//
LOCUS       DAZFFI010000476          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFFI010000476 DAZFFI010000000
VERSION     DAZFFI010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.480"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..541)
                     /gene="fimE"
                     /locus_tag="ACHJ12_005518"
     CDS             complement(<1..541)
                     /gene="fimE"
                     /locus_tag="ACHJ12_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4993111.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFA"
BASE COUNT          203 a          169 c          142 g          157 t
ORIGIN      
        1 cggcaaaacg agcagcatta ctggcggtat aacgtacagt atggcgaata tttcgatgcc
       61 cgagataatc ctgaattaaa cgggtatcgg caccacgctc cgccagttca taaccacaag
      121 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc
      181 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag
      241 cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt
      301 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
      361 ttattcgacc ttcattaagg tcaagatcct gataatgcag atcaagcagt tcactaatac
      421 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
      481 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
      541 caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg
      601 gtcaaaatta gcccaataga taaaaagata accacacacg gagtgatgtg gctatcagtc
      661 aattacatta a
//
LOCUS       DAZFFI010000477          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFFI010000477 DAZFFI010000000
VERSION     DAZFFI010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.481"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..311)
                     /locus_tag="ACHJ12_005519"
     CDS             complement(54..311)
                     /locus_tag="ACHJ12_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993112.1"
                     /translation="MKNEFILMKLVARGLLDIRIAADSGNVKACHMLSDFIHTLPYAI
                     ERVLKGEIDYQYVMDNLNERAKIKNMEGWLANALRDINASE"
     gene            complement(313..>668)
                     /locus_tag="ACHJ12_005520"
     CDS             complement(313..>668)
                     /locus_tag="ACHJ12_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993113.1"
                     /translation="GSPGGWGPEDEEKARSRGRGSDTEAKIKDLDLLHSPETIGSRPD
                     LAKLTDSELLESVINPKSGDRVTVNTKTNKVMDGNSRVYELKKRVDDPNSSITGETTI
                     PVETYTPDDSFFWDM"
BASE COUNT          197 a          157 c           94 g          220 t
ORIGIN      
        1 ataaaaaccg ggagaattaa tatacttccc ggcttattat ttatcaaagg atattactct
       61 gacgcattta tatctctcaa cgcattagca agccaacctt ccatgttttt tatcttggcc
      121 ctttcattta aattatccat cacgtactga taatcaattt caccttttaa aaccctctca
      181 atagcatacg gtaaagtatg tataaaatca gataacatat gacaagcttt cacattacca
      241 gaatctgctg ctattcttat atccagcaat cctctggcga ctaacttcat gagaataaat
      301 tcatttttca tattacatgt cccaaaaaaa agaatcatca ggtgtgtaag tttctactgg
      361 tatcgtagtt tcaccggtta ttgagctatt aggatcatca actctttttt tcaactcgta
      421 tacacgagaa ttaccatcca ttactttatt tgtttttgta ttaacagtta ctcgatcacc
      481 agacttagga ttaataacag actctaacaa ctcactgtct gttagtttag caagatcagg
      541 acgcgagcca atagtttccg gcgaatgaag aagatccaga tcctttattt ttgcttctgt
      601 atcagaacca cgcccccgac tgcgagcctt ttcctcatcc tctggacccc atcctcccgg
      661 acttcctg
//
LOCUS       DAZFFI010000478          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFFI010000478 DAZFFI010000000
VERSION     DAZFFI010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.482"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..178)
                     /locus_tag="ACHJ12_005521"
     CDS             complement(5..178)
                     /locus_tag="ACHJ12_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993114.1"
                     /translation="MSTPLEKRSPPYQMRLPEDFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
     gene            309..>665
                     /locus_tag="ACHJ12_005522"
     CDS             309..>665
                     /locus_tag="ACHJ12_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4993115.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALNIGEKNQLIASVENSYDSRA
                     PHKTGAGIATPLTIEAIQTPQASFFVSAHTHTYTMVGCMGLTSVRLVPLIASCSNPVQ
                     STASELATSCGGYIPSI"
BASE COUNT          155 a          172 c          155 g          183 t
ORIGIN      
        1 gtttttatcc tttgggttct atgccgcgtt gctgtaattc tttgcggaga acgcgtttaa
       61 tccaagtggc taaagatgtg tcgccatcgg ccttagctgc ttcttctagc tgcgctctga
      121 agtcttccgg taatcgcatt tggtatggag gcgaacgttt ttctaatggt gttgacatgg
      181 taattaccct tcattactat acctacatgg taattaccat tgtacttgcc attacacaaa
      241 aatcaacgcc ccgcagtgct gggaacacat acggggcgtc taaccaacaa cgtaaactag
      301 gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa acggcctttg
      361 tgtggcgttt tctggccctg aatatcggag aaaaaaacca gttgatcgcc agtgtcgaaa
      421 atagctatga ttcccgcgca cctcataaaa cgggtgccgg gattgctacc ccgctgacaa
      481 tcgaggcgat acagacgccg caagcgtctt tttttgtatc ggcgcacacg cacacctata
      541 caatggtggg ctgtatgggg ctgacttcgg tcaggctggt tcccttgatt gccagttgta
      601 gcaaccctgt acagtccacc gccagcgagc ttgctacctc ctgcggtggt tacattccat
      661 caatc
//
LOCUS       DAZFFI010000479          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFFI010000479 DAZFFI010000000
VERSION     DAZFFI010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.483"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..85
                     /locus_tag="ACHJ12_005523"
                     /pseudo
     CDS             1..85
                     /locus_tag="ACHJ12_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            334..>664
                     /locus_tag="ACHJ12_005524"
     CDS             334..>664
                     /locus_tag="ACHJ12_005524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993116.1"
                     /translation="MSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGGKITISENGK
                     VTPPSHQHSEELIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDSVV
                     REALLRDF"
BASE COUNT          201 a          127 c          177 g          159 t
ORIGIN      
        1 cataatttta gaggcgcaaa atgtgttgat aacctacaaa ggaaagctac cgggaagaat
       61 tactgattct ctgaagatgc cgccgtcaac attgcggtca gaaaaagatg atgttgaatc
      121 acgtattgaa aaactggagc gctatatcgc tgaattgaaa aaaagcaccc caacaaaaaa
      181 tgaggtgctt gcagcagatg aaatgaaaga agctattctt gatcgcgcgg cgcatctaag
      241 ctgcgcttca cggttgaaag agcatcttca gcagcctgaa aaaaacgccg cgatgagcag
      301 tttgcggcgt tttacgatta ttgccggaaa gtgatgagca gaaatctcgc agagtgtttc
      361 agtattcata atgataattt cagtgagctg gaatgggagt gtaaccggcc atcctttgtt
      421 gtatccggtg atggtgggaa aataaccatc tcagaaaatg ggaaagtaac acctccatcg
      481 caccagcaca gtgaggagct cattgaattt gccattgatt acctgaagaa caataaaaaa
      541 caggggctga tgcagcgcat tggtcgttgc atggggtatc tgcaggtagc cgctgagatt
      601 gaagcgctgg ccagtggtgc ggacaaggat tcagttgtgc gggaggcact tcttcgtgat
      661 tttg
//
LOCUS       DAZFFI010000480          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFFI010000480 DAZFFI010000000
VERSION     DAZFFI010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.484"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>661
                     /locus_tag="ACHJ12_005525"
     CDS             <1..>661
                     /locus_tag="ACHJ12_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4993117.1"
                     /translation="FIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRHSLRCS
                     PNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAGVTDNA
                     YRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVALWLVQT
                     DAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGANEAFD
                     LL"
BASE COUNT          136 a          154 c          224 g          147 t
ORIGIN      
        1 gttttattga ggcaacggtt gacgtacagg gcggtaaaaa tcgccgtttt gttgtgcaga
       61 tcaccggata cggagagcag ggggaacgct ggattgttga tcgctacaac atccggcatt
      121 cactgcgctg cagtcccaac ggtgaaagtc tgccggttga tccggcggca tatccggagg
      181 actgggattt gttgctgacg gatgtgttcc ataaaacatg gccgctggct tctgatccgg
      241 atgtgcgcat gcgtctgatg gccatggcgg tggatacggg aggggaagcc ggggtgacag
      301 ataacgccta tcgtttctgg cgtcgttgcc ggagtgacgg actgggcaac agggtgtttc
      361 tgttcaaggg ggatggtctt cgccgtgaca ggctgattaa ccgtaccttc ccggataata
      421 ccggcagaag tgcccgccgt gccagagcca gtggcgatgt cgcgctgtgg ctggttcaga
      481 cggatgcgtt taaggaccgt gtaaataatg ccctgtggcg tgacacacca gggccgaact
      541 atatccactt tcccgactgg ctggggcggt ggttttacga tgagctgacc tatgaagagc
      601 gcggcagtga cggaaaatgg cgaaaaccgg gcaggggcgc taacgaggcg tttgacctgc
      661 t
//
LOCUS       DAZFFI010000481          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFFI010000481 DAZFFI010000000
VERSION     DAZFFI010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.485"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..360
                     /gene="iraM"
                     /locus_tag="ACHJ12_005526"
     CDS             37..360
                     /gene="iraM"
                     /locus_tag="ACHJ12_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4993118.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(463..630)
                     /locus_tag="ACHJ12_005527"
                     /pseudo
     CDS             complement(463..630)
                     /locus_tag="ACHJ12_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
BASE COUNT          221 a          127 c          111 g          198 t
ORIGIN      
        1 gaactatatt aaataccgtg ggataagaca taacaaatga agtggatagt aattgacacg
       61 ataattcaac cttcatgcgg catatctttt tcagccatat ggggtaatat gaaaatgatc
      121 atctggtatc aatctactat atttctccct cctggcagta tatttacacc ggttaaatct
      181 ggaattatcc ttaaagataa agaatatcct attactattt ataacatcgc accattcaac
      241 aaggatttat ggagtctact caaaagcagt caagagtgtc ctccaggaga aagcaaaata
      301 acaaataaat gtttgcataa tagttgcatt ataaaaatat gcccatatgg gctcaagtaa
      361 agggttttat ataaatatgc tcgatatgct attaataata cctacataaa taaaacaacg
      421 ggcgtgttat acgcccgttg caatattaaa cacatgtagt gattacatgt tcttgatgat
      481 cgcatcacca aattctgaac atttcagcag tttagcgcct tccatcagac gctcgaagtc
      541 ataggttacg gtcttcgcat tgattgcgcc ttccatacct ttaacaatca ggtctgcggc
      601 ttcagtccag cccatatggc gtaacattag ttagatgact aaatcgtaac tatttga
//
LOCUS       DAZFFI010000482          651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFFI010000482 DAZFFI010000000
VERSION     DAZFFI010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.486"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005528"
     CDS             <1..255
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060295.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4993119.1"
                     /translation="TALKDDPMSGHVFIFRGRSGSQVKLLWSTGDGLCLLTKRLERGR
                     FAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            305..>651
                     /locus_tag="ACHJ12_005529"
     CDS             305..>651
                     /locus_tag="ACHJ12_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4993120.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPA"
BASE COUNT          149 a          190 c          182 g          130 t
ORIGIN      
        1 acggcgctga aagacgatcc catgtccggc catgttttca ttttccgggg ccgcagcggc
       61 agtcaggtta aactgctgtg gtccaccggt gacggactgt gcctcctgac caaacggctg
      121 gagcgtgggc gcttcgcctg gccgtcagcc cgtgatggca aagtattcct tacgcaggcg
      181 cagctggcga tgctgctgga aggtatcgac tggcgacagc ctaagcggct gctgacctcc
      241 ctgaccatgc tgtaaatctc tttatcctgg ttgtcacaga ataagcccgg taaaatacgg
      301 gcttatgaac gacatttctt ctgacgacat cttcctgctg aaacagcgcc tggccgaaca
      361 ggaagcgctg atccacgccc tgcaggaaaa gctgagcaac cgggagcgcg aaatagacca
      421 tctgcaggcg cagctggata aactccgccg gatgaacttc ggcagtcgtt ccgaaaaagt
      481 ctcccgccgt atcgcacaaa tggaagccga tctgaaccga cttcagaaag agagcgatac
      541 gctgactggt agggtgtatg acccggcagt acagcgtccg ttgcgtcaga cccgcacccg
      601 taagccgttc cctgaatcac taccccgtga cgaaaagcga ttgttgcctg c
//
LOCUS       DAZFFI010000483          651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFFI010000483 DAZFFI010000000
VERSION     DAZFFI010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.487"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005530"
     CDS             <1..255
                     /gene="tnpB"
                     /locus_tag="ACHJ12_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060295.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4993121.1"
                     /translation="TALKDDPMSGHVFIFRGRSGSQVKLLWSTGDGLCLLTKRLERGR
                     FAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
     gene            305..>651
                     /locus_tag="ACHJ12_005531"
     CDS             305..>651
                     /locus_tag="ACHJ12_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4993122.1"
                     /translation="MNDISSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPA"
BASE COUNT          146 a          193 c          185 g          127 t
ORIGIN      
        1 acggcgctga aagacgatcc catgtccggc catgttttca ttttccgggg ccgcagcggc
       61 agtcaggtta aactgctgtg gtccaccggt gacgggctgt gcctcctgac caaacggctg
      121 gagcgtgggc gcttcgcctg gccgtcagcc cgtgatggca aagtgttcct tacgcaggcg
      181 cagctggcga tgctgctgga aggtatcgac tggcgacagc ctaagcggct gctgacctcc
      241 ctgaccatgc tgtaaatctc tttatcctgg ttgtcacaga ataagcccgg taaaatacgg
      301 gcttatgaac gacatctctt ctgacgacat cctcctgctg aaacagcgcc tggccgaaca
      361 ggaagcgctg atccacgccc tgcaggaaaa gctgagcaac cgggagcgcg aaatagacca
      421 tctgcaggcg cagctggata aactccgccg gatgaacttc ggcagtcgtt ccgaaaaagt
      481 ctcccgccgt atcgcacaaa tggaagccga tctgaaccgg cttcagaaag agagcgatac
      541 gctgactggt agggtgtatg acccggcagt acagcgtccg ttgcgtcaga cccgcacccg
      601 taagccgttc cctgaatcac taccccgtga cgaaaagcga ctgttgcctg c
//
LOCUS       DAZFFI010000484          651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFFI010000484 DAZFFI010000000
VERSION     DAZFFI010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.488"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..523)
                     /locus_tag="ACHJ12_005532"
     CDS             complement(<1..523)
                     /locus_tag="ACHJ12_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFO4993123.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINSVILNDVSWDVVIE
                     STEVNDLNLFVWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNF"
BASE COUNT          221 a          138 c           95 g          197 t
ORIGIN      
        1 taaaattcat aaatattttt ttagactggt caatagagac atccattctc tgagcagaat
       61 actcatgact ataacccaaa agatacggta ttatcttatt aaagcgatat ttgtcagatt
      121 cacaacaaaa ctccttgccc tttataaaaa agctactaac tacgtatctt tcgaaaaatc
      181 tccaaacaaa caaattcaag tcattcactt cggtactttc aatcaccaca tcccatgata
      241 catcattcaa tattacacta ttaataatgc tcactccatt tgaggacgaa aaagcatcaa
      301 cttcagcagt ctgaaataaa atttgcgtat caatatcaat accagaaaac catatttcag
      361 aatcacaatt ctgacacttt attgagtgta aaaaaacaga gtttgagtct atgaattttc
      421 ctgactctgt gcggacacag actggatatt ttgcggataa aataatttcc ggcaggcctg
      481 attgacgtat gagtgctctt tctctagtat ccatcgcaca cataggaacc tcctcaaagg
      541 attctatgga cagtcgatgc agggagctag tccctccctg catcggcgat tttcaagttc
      601 aaattttaac gtggcattaa ccacataatt aatatactcg ttatgtggtt a
//
LOCUS       DAZFFI010000485          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFFI010000485 DAZFFI010000000
VERSION     DAZFFI010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.489"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>647)
                     /locus_tag="ACHJ12_005533"
     CDS             complement(<1..>647)
                     /locus_tag="ACHJ12_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4993124.1"
                     /translation="FNRLLGLGNELLQQRQGLASVPLRLPQATLDDKQQGALNNTERQ
                     LALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAWRNNEANKPKSRAG
                     KSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKTLTEIQKQELLRNA
                     ALIDQKKIREQLRAREETLKNDNADARASNDAELLGYGQGERVRERMRELQQIRD"
BASE COUNT           99 a          215 c          153 g          180 t
ORIGIN      
        1 gtcacgaatc tgctgcagct cccgcatccg ttcacggacc cgctcaccct gcccgtaccc
       61 cagcagctca gcgtcatttg atgcccttgc gtctgcatta tcatttttca gggtctcctc
      121 ccgggcccgt aactgctccc ggattttctt ctggtcaatc agggcagcat tgcgcagcag
      181 ctcctgcttc tgtatctccg tcagggtttt cagctcgccc tgcgaggtct ggtatttcag
      241 ttttgccagt tctgtattct tcccggccag tgccagttgt tccttctgct gcttcagcag
      301 acgggaaaaa ctgtcttccg ctttttccgc ccctgatttt ccggcccggg atttgggttt
      361 attcgcctca ttattgcgcc aggcttccat cgaattacgg atgtaacgct gcctcgcctc
      421 ctgatacgta tcccccacca gaccaaggtc atccgccgca taccccagcc gggcacgctc
      481 tttttcttcc cctttcagcc gggacagggc aagttgacgc tctgtgttat tcagggcgcc
      541 ctgctgttta tcatccagag tggcctgtgg cagtcgcagc ggtacactcg ccagtccctg
      601 acgctgttgc agcagttcat tacccagccc cagcaggcgg ttgaatt
//
LOCUS       DAZFFI010000486          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFFI010000486 DAZFFI010000000
VERSION     DAZFFI010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.490"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHJ12_005534"
     CDS             <1..216
                     /locus_tag="ACHJ12_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4695 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993125.1"
                     /translation="WLQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASG
                     SVLARRLQISHYPVLITDNGLSQVPAP"
     gene            213..>646
                     /locus_tag="ACHJ12_005535"
     CDS             213..>646
                     /locus_tag="ACHJ12_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4993126.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVIGGECWLIQAKRYGRTIRPAHIDAFGELL"
BASE COUNT          128 a          163 c          211 g          144 t
ORIGIN      
        1 tggctgcaga tgaatgcagc agtactcagg gagcggaagg cggcgggaat ggtagtgaat
       61 gtggcggatg agagcagtct gcgcgtactg cgtgagctgg caccgggggt ggtgatggtt
      121 ccggcatccg gttctgtact ggcccgcagg ttacagataa gtcattaccc ggtgctgata
      181 acggacaacg gcctgtcaca ggtacctgca ccatgatgcc gctgtctgcc acttctgctg
      241 cgctctctgc ttctccggta ctgacagcct gcctggttct cagcctgctg gctgttgtcc
      301 tggggccggc actgtggcgc agccggcgaa aaaaagcgtg tgtgagaaca cgccggcatc
      361 agcgttatcg tgccacggcc tcacgggtaa tgaagcggct gaacgaattg cagggcgatg
      421 cccggcggct gaaatatctg cgcaaaatca gtccgtatgt gtttgaggaa ctgttgctgc
      481 tggcttttga acaacaggga atgcctgtga tccgtaaccc ctcatacagc ggcgatggtg
      541 gtcttgatgg tcaggtggtg attggcggtg agtgctggct gatacaggcc aaacgttacg
      601 gaagaaccat tcgtcccgca cacattgacg cgtttggcga actgct
//
LOCUS       DAZFFI010000487          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFFI010000487 DAZFFI010000000
VERSION     DAZFFI010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.491"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>637
                     /locus_tag="ACHJ12_005536"
     CDS             <1..>637
                     /locus_tag="ACHJ12_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993127.1"
                     /translation="LETVWMQISSTLKGALDINSLIIDYDNEDVQSYWEDVVLPNLND
                     NGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAII
                     SSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDSLIEWKPSY
                     YQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRCRNID"
BASE COUNT          225 a           92 c          129 g          191 t
ORIGIN      
        1 atttagaaac ggtttggatg cagatatcat cgactctcaa aggtgctctg gatatcaaca
       61 gcttgatcat tgactacgat aatgaagatg tccaaagtta ttgggaagac gttgtattac
      121 cgaacttaaa tgataatggt aataaagctc tcacaagaga tgaaacaata gcctatatta
      181 aagacagtaa gccttgtaga agaattaaaa gattaataca tgatgatgct gtgttggagg
      241 atcaaaagga taaaaacttc attgcaaaaa tgattgcaga atatgctgtg tctagaaatg
      301 caattatttc tagcaacaca tctggcaatg tcattcgggg tgatgaaagc ggagtaaaat
      361 ggattcaatg tggtaatatc tttgtctccc tatttcataa ggttcaagat gatcatgaaa
      421 acgatggaga taggatttgg caaactctca atgattctct cattgaatgg aaaccatctt
      481 attatcagtt aataaaatct gaaattcaga atgcaatcga agctgaggct ttatcttttg
      541 taaatcattt ggctaacgat cattacggtc aagctgcgtg gttaaatgag atattaaaat
      601 cagactcgcc tgatattaga tgtagaaata ttgactt
//
LOCUS       DAZFFI010000488          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFFI010000488 DAZFFI010000000
VERSION     DAZFFI010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.492"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..>634
                     /locus_tag="ACHJ12_005537"
     CDS             246..>634
                     /locus_tag="ACHJ12_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4993128.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQA"
BASE COUNT          178 a          141 c          142 g          173 t
ORIGIN      
        1 agctgttttc attactttta ttttaccatt actctttcca gtttttgccc acggtagcaa
       61 atatcctgtt gcattgtata tgcaggaacc gaatatagcc aaagcatggc gacagagaaa
      121 tgatagcgct atcacaaaga ttggtagcgt tatcagatag cgctatcaaa tgcgacaatc
      181 atcacattaa ttaatgcgta aaatatctac tctcctgtta attatcatcg ggagttatca
      241 gtcgcatgtt aaaaaatcta ttaaatacag aggtcgttca ggtcgtggaa caggccaaag
      301 actggcggga agccgtggcg atttcatgcc ggccgttgat tgaaaacggt tctatcgaac
      361 cgcgttatgt tgatgcgatc tatcgctcgc acgacactat cggtccgtac tatgtggttg
      421 gccctggtat tgcgatgcct catgcgcgcc cggaagaggg agctaacaag ctgtcattag
      481 cactgaccct gatcccttca ggtgtgaatt ttgatgccga tgaaaacgat ccagtgaaat
      541 tactgattgt gctggctgca accgacagta ccagccacat tgaagcgatt tctcaactcg
      601 ccaagttatt cgataacgaa aaagacatac aggc
//
LOCUS       DAZFFI010000489          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFFI010000489 DAZFFI010000000
VERSION     DAZFFI010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.493"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..>634
                     /locus_tag="ACHJ12_005538"
     CDS             246..>634
                     /locus_tag="ACHJ12_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4993129.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPSIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQA"
BASE COUNT          180 a          142 c          139 g          173 t
ORIGIN      
        1 agctgttttc attactttta ttttaccatt actctttcca gtttttgccc acggtagcaa
       61 atatcctgtt gcattgtata tgcaggaacc gaatatagcc aaagcatggc ggcagagaaa
      121 tgatagcgct atcacaaaga tcggtagcgt tatcagatag cgctatcaaa tgcgacaatc
      181 atcacattaa ttaatgcgta aaatatctac tctcctgtta attatcatcg ggagttatca
      241 gtcgcatgtt aaaaaatcta ttaaatacaa aggtcgttca ggtcgtggaa caggtcaaag
      301 actggcggga agccgtggcg atttcatgcc gaccgttgat tgaaaacggc tctatcgaac
      361 cgcgttatgt tgatgcgatc tatcactctc acgacactat cggtccgtac tatgtggttg
      421 gccctagtat tgcgatgcct catgcgcgcc cggaagaggg ggctaacaag ctgtcattag
      481 cactgaccct gatcccttca ggtgtgaatt ttgatgccga tgaaaacgat ccagtgaaat
      541 tactgattgt gctggctgca accgacagta ccagccacat tgaagcgatt tctcaactcg
      601 ccaagttatt cgataacgaa aaagacatac aggc
//
LOCUS       DAZFFI010000490          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFFI010000490 DAZFFI010000000
VERSION     DAZFFI010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.494"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..294
                     /locus_tag="ACHJ12_005539"
     CDS             25..294
                     /locus_tag="ACHJ12_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4993130.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEPQPDGEMMM"
     gene            369..584
                     /locus_tag="ACHJ12_005540"
     CDS             369..584
                     /locus_tag="ACHJ12_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4993131.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          157 a          138 c          196 g          136 t
ORIGIN      
        1 aacccctttc aacgagaaaa tcccatgtca gaaattacat ccctggtcac tgcagaggca
       61 gtgaaggaag tcctgcgctc tgaagaagtc ctgagcgcac tgaaacaaaa actccgccag
      121 aatcttgagg cgcgtcttga tgcagaagtg gatgccattc tggatgaact gctgggcgca
      181 ccgactgttc cggagccgga aggcattgcg gatgacagtg ctgtttcaga tggcgtcggg
      241 tctcagcctg acggtagtga agagcctcag cctgacggcg aaatgatgat gtaaccatgc
      301 gcaggggtgt cggtgtgagc tgatgcccca cttgttgttg tgagcttccg gattgcggga
      361 ggcggggtat gtaccagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg
      421 tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct cagtgggcgg
      481 caataggcgt gctggggagt ctgctgtttg ggctgctgac atatctgacg aacctgtatt
      541 tcaaaattaa agaagaccgg cgtaaggcgg cgcggggaga gtagacgatg aataaacaat
      601 acgaactggt tgttaaaggg ataaata
//
LOCUS       DAZFFI010000491          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFFI010000491 DAZFFI010000000
VERSION     DAZFFI010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.495"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..443
                     /gene="rhuM"
                     /locus_tag="ACHJ12_005541"
                     /pseudo
     CDS             282..443
                     /gene="rhuM"
                     /locus_tag="ACHJ12_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          172 a          144 c           99 g          210 t
ORIGIN      
        1 aatgatatta tagccagaca ctatccggaa atgttacctt cacaatagag tactaagaat
       61 tgattcaatt atgtatttta gcttcattcc ttcatgcaaa atcatttggg ggttcttatg
      121 ggggttactc atttatccaa cacatacaaa ttatttaaga tcaatgcatt aatacttata
      181 ttcgactcct gtgatctccg ccaaaatgcc tctcctgacg ttctccaacc ccttgtaata
      241 ctgtcaatcc agatattttc tactgctcaa tattcaatcg gcttcaggaa tacctggtct
      301 aaggttttgt gatggacgat gagcggctga agaatccgcc tgtcggttct tctgtcctcg
      361 aacgcatccg cgatattcac gccagtatgc gccgtgttta tttgcaggtg atttgccccc
      421 ctgctgactc tcagccaact gtatcgccat taaggtgaag atgttgtttt actcgcccca
      481 gaacaccctt ttttatacga ctattctatt tctttaaccg ctggcgaaaa aaactgacta
      541 taaacttcac caataatcag actgccatca agattaattt gttcatttaa tatatcaaat
      601 caacttgttt tatttttact atcaa
//
LOCUS       DAZFFI010000492          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFFI010000492 DAZFFI010000000
VERSION     DAZFFI010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.496"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..>624)
                     /locus_tag="ACHJ12_005542"
     CDS             complement(176..>624)
                     /locus_tag="ACHJ12_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO4993132.1"
                     /translation="DGTLTSADGATKTIAVNDGLVALSGRTDRIDAAVGSVDRRVTKN
                     TQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATA
                     ALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF"
BASE COUNT          139 a          193 c          137 g          155 t
ORIGIN      
        1 tccctcgcca gtattatcct gtacccctca agcatctgct gcaggccata ctccagataa
       61 cggttgcggg tcgccagaat cactttcatc gctgtcacct gtttcatgct cacatgagaa
      121 aaaacggcag gcaaaactcc acgcctgccg tcatgcttca tgtcactgtc agcaattaaa
      181 actcaaagtt cacacctacg ttgtaggaca catcgccatc actcgctgcc acacctgctt
      241 tcgctgctgt ctgctcattg aaacggtaac caacacccac ggcaacagcc tgtttatcac
      301 tgtacccccc cagggcagca gtggcgttga atttccccac actgtacggc tggaaaagcc
      361 ccgccagcgc tgcactctgt gctgctgcac gcttcatctc ttcgtgattc tcacgaattt
      421 gacgctgctg gctgttcagg cgtgcattat gttcctgcaa ctggcgagta ttggactgaa
      481 tagcctgtgt atttttagta acccggcggt caacgctacc aactgcagca tcaatacggt
      541 ctgttctgcc ggacagagca acgagaccgt cattaacagc aatcgttttt gtagcgccat
      601 cagcgctggt cagggttccg tccg
//
LOCUS       DAZFFI010000493          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFFI010000493 DAZFFI010000000
VERSION     DAZFFI010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.497"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>617
                     /locus_tag="ACHJ12_005543"
     CDS             <1..>617
                     /locus_tag="ACHJ12_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4993133.1"
                     /translation="DGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTV
                     NGETAEQWQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQ
                     HETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLV"
BASE COUNT          152 a          183 c          195 g           87 t
ORIGIN      
        1 cggacggcag cagaaacggg acacagtacg atgcgtgggg aaaagccatc tgtaccacgc
       61 agggcggtct gacgcgcagt atggaatacg atgctgccgg acgggtcatc cgcctgacca
      121 gtgaaaacgg cagccacacc accttccgtt acgatgtact cgaccggctg atacaggaaa
      181 ccggctttga cggccgcaca cagcgttatc accacgacct gaccggcaaa cttatccgca
      241 gcgaggatga ggggctggtc acccactggc actatgacga agcagaccgc ctcacgcacc
      301 gcaccgtgaa tggcgaaacc gcagagcagt ggcagtatga cgaacgcggc tggctgacag
      361 acatcagcca tatcagcgaa gggcaccggg tgacggtgca ttacgggtat gatgagaaag
      421 gccggctgac cggtgagcgt cagacggtgc atcacccgca gacggaagca ctgctctggc
      481 agcatgagac cagacatgcg tacaacgcgc aggggctggc gaaccgctgt ataccggaca
      541 gcctgcccgc cgtggaatgg ctgacctacg gcagcggtta cctggcaggc atgaaactcg
      601 gcgacacacc gctggtg
//
LOCUS       DAZFFI010000494          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFFI010000494 DAZFFI010000000
VERSION     DAZFFI010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.498"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..526
                     /locus_tag="ACHJ12_005544"
     CDS             <1..526
                     /locus_tag="ACHJ12_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4993134.1"
                     /translation="VAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTVN
                     LGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLKN
                     TKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATP
                     GNVYAQADYKIAYE"
BASE COUNT          194 a          131 c          137 g          153 t
ORIGIN      
        1 tgttgctggc gtagcaatga ttctttccaa taacgtattt gctgacgaag gtcacggcat
       61 tgttaaattt aaaggtgaag ttatctctgc tccatgttca attaaaccag gtgacgaaga
      121 cttaactgta aatctgggtg aagtagcaga taccgttctg aaaagcgacc agaaatcttt
      181 ggctgagcct ttcactattc acctgcaaga ctgcatgttg agccagggtg gaaccactta
      241 ttcaaaagcg aaggttactt tcaccacagc gaatactatg acgggtcaga ctgacctgct
      301 gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc gttggcgtac gtattctgga
      361 tagccagagc ggcgaagtga ctttaggtac tccggtagta attacattca acaatactaa
      421 cagctatcag gaactgaact tcaaagctcg tatggaatcc ccaagcaaag atgctacccc
      481 gggcaacgtt tacgctcagg ctgactataa gattgcttac gagtaatcac attaggccgg
      541 gttcgcccgg ccacatcatt aacggattaa tgataagtgg aacagatgta taaaaaatta
      601 aaattaacca caata
//
LOCUS       DAZFFI010000495          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFFI010000495 DAZFFI010000000
VERSION     DAZFFI010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.499"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>601)
                     /locus_tag="ACHJ12_005545"
     CDS             complement(<1..>601)
                     /locus_tag="ACHJ12_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4993135.1"
                     /translation="KTEGIKVKVGQLVRLLNVPMEKATKFHEYSNGKEHADALKEAWK
                     ENHGAAGREWVKWLSGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAAL
                     VLSGHVTGWAVQECRDAILHNFNAWVKVFGTGNKEHKQIIEQAEAFLAAYGMSRFAPV
                     NYDPASLPIPELYGYRESDGRYDEPVLFYVLPDPFGSHVA"
BASE COUNT          117 a          196 c          140 g          148 t
ORIGIN      
        1 tcgccacatg tgagccgaac ggatccggca gcacgtagaa caaaacaggt tcgtcataac
       61 gcccgtcgct ttccctgtag ccgtacagtt caggaatcgg cagactggcg gggtcataat
      121 tgacaggggc aaacctgctc atcccatagg ccgcaagaaa cgcctcagcc tgttcaataa
      181 tctgcttgtg ctccttgttc cccgtaccga acaccttcac ccaggcatta aagttgtgca
      241 gtatcgcgtc gcggcattcc tgcacggccc agccagttac atgaccggaa agcacaagcg
      301 cggcctccag tatggcgaaa cgttcaccca cgcggtggac ctgctcgccg tagctctccg
      361 gtatcaggtt gcgccaccgt tcgcggcatt cccttaccgt atcctttgcc tcctgctggt
      421 ggcctgacag ccatttaacc cactcacgac ccgccgcccc gtggttttcc ttccaggctt
      481 cttttaacgc gtccgcatgt tccttaccgt tgctgtattc gtgaaatttc gtggctttct
      541 ccatcggtac attaagcagg cggacaagct gccctacttt tacctttatc ccctcggttt
      601 t
//
LOCUS       DAZFFI010000496          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFFI010000496 DAZFFI010000000
VERSION     DAZFFI010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.500"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            336..>598
                     /locus_tag="ACHJ12_005546"
     CDS             336..>598
                     /locus_tag="ACHJ12_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4993136.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIP"
BASE COUNT          151 a          112 c          166 g          169 t
ORIGIN      
        1 gaacggtggc agtgagaatg gaacagaaaa aaatcagatg cggaaggcgg aaatacacag
       61 cgggtgcgat gtgcttcatg ctgtttgtat ggctgggtgg tgtggtgaca ttctgtgtgc
      121 tggccgctct gataatgagg tgaaaacgga agagagttta cttccttaat tcgaactttc
      181 tgataactga gaacttgtta aatgctcgtg ggtttatata tttaaatcaa ttggttaatg
      241 ctgacttcat ctttatcagt caaagtttgt tttagtatga tgattgtgcc cactgtttat
      301 tgttgtccgg gagaaaatac agggaattcc agggaatgaa caaaatatac tcactcagag
      361 ttaatgacag aaaagaactg gttgctgtac cggaaacggt ggggggcagg aaaaagtcgt
      421 ccggaattcc cggtgcgggg atgctttccc gtcttcttct ggcctcagga gccgttgccg
      481 gcgttctgtt ttcttctcct gcgctggcat ccgttgtcgg gaatactctg ccatggcaga
      541 cctacaggga ttttgctgaa aacaaagggg cgtttcatgc cggggccaca aatattcc
//
LOCUS       DAZFFI010000497          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFFI010000497 DAZFFI010000000
VERSION     DAZFFI010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.501"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            335..>597
                     /locus_tag="ACHJ12_005547"
     CDS             335..>597
                     /locus_tag="ACHJ12_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4993137.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIP"
BASE COUNT          148 a          111 c          170 g          168 t
ORIGIN      
        1 gaacggtggc agtgagaatg gaacagaaaa aaatcagatg cggaaagcgg aaatacacag
       61 cgggtgcgat gtgcttcatg ctgtttgtat ggctgggggg tgtggtgaca ttctgtgtgc
      121 tggccgctct gataatgagg tgaaaacgga agagagttta cttccttaat tcgaactttc
      181 tgataactga gaacttgtta aatgcctgtg ggtttatgta tttaaatcaa ttggttaatg
      241 gtgattttat ctttatcagt caaagttcgt tttagtatga tgattgtgcc cgctgtttat
      301 tgttgtccgg gagaaaatac agggaatcca ggggatgaac aaaatatact cactcagagt
      361 taatgacaga aaagaactgg ttgctgtacc ggaaacggtg gggggcagga aaaagtcgtc
      421 cggcattccc ggtgcgggga tgctttcccg tcttcttctg gcctcaggag ccgttgccgg
      481 cgttctgttt tcttctcctg cgctggcatc cgttgtcggg aatactctgc catggcagac
      541 ctacagggat tttgctgaaa acaaaggggc gtttcatgcc ggggccacaa atattcc
//
LOCUS       DAZFFI010000498          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFFI010000498 DAZFFI010000000
VERSION     DAZFFI010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.502"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..>588)
                     /locus_tag="ACHJ12_005548"
     CDS             complement(33..>588)
                     /locus_tag="ACHJ12_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4993138.1"
                     /translation="FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLG
                     SVFMQLELGDKYRSQFFTPWSVASMMAQMQMGDVKALFENKPFITLSEPACGAGSMIL
                     AMADTLNRSGYPAYRRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRV
                     LLTPGHYLGNWDHRLRHLQEQNVA"
BASE COUNT          150 a          186 c          126 g          126 t
ORIGIN      
        1 cgaagactgg cgcagtgata aaacattctg catcatgcca cattctgctc ctgaagatga
       61 cgcagacgat ggtcccagtt tcccaggtaa tgcccgggcg tcagcagtac gcggcgtcgc
      121 tcattacaga gcgagttacc aatcaccact tctccggcca caccacacaa cgacagttgt
      181 atatacgcca tgccggctgc cagcgggtca atatccgttg ccgacaccca catcctgcga
      241 tatgccggat accccgaacg gttcagggtg tcagccattg ccagaatcat actgccggca
      301 ccacaggcag gttcactgag cgtgataaag ggtttgttct caaacaacgc cttcacatct
      361 cccatctgca tctgtgccat catgctggca acactccagg gcgtgaaaaa ctgactccgg
      421 tatttatccc ccagttccag ttgcatgaac acactcccca gaaaatcaca gaatccctgc
      481 tccagtccct caatgacatg agcaagcaaa tgcgcaatgt ggataacatc ctgcttttca
      541 taaccactga cgatccgcag atacttctgt tcccgggcct cactgaac
//
LOCUS       DAZFFI010000499          574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFFI010000499 DAZFFI010000000
VERSION     DAZFFI010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.503"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..278
                     /locus_tag="ACHJ12_005549"
     CDS             <1..278
                     /locus_tag="ACHJ12_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4993139.1"
                     /translation="GVDRYSTEVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQP
                     PQNAQQSTGKNGQASSGSGGMPPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            327..>574
                     /locus_tag="ACHJ12_005550"
     CDS             327..>574
                     /locus_tag="ACHJ12_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO4993140.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTL"
BASE COUNT          137 a          138 c          165 g          134 t
ORIGIN      
        1 atggtgttga tcgttattcc acggaagttg ttgtgagtgt caatggtacc ctgcagatgc
       61 tggggtctcc gcgtcagcag cagggtggtg gaaatgtttc atctgctcct gctcagggag
      121 gatggggaca accaccacaa aatgcacaac aatcaacggg aaaaaacggt caggcttcct
      181 caggaagcgg gggaatgccg ccgatggact ttgacgatga tatccccttt atggggcgtg
      241 ggtatggcgt tgaacgacgg attatccatg tcatctgatg ttaaccacgg ggcttaccgc
      301 cccactttta attcaaggag gcggttgtga atgaaaacac acccactacc cccacgccac
      361 atgatgcggc gttcagagcg atgatggaat gtccctctgt tgcgagggat ttcctgaaag
      421 gcgcgttgcc cccggaacaa ctgaaccggt gcgatatgaa caccctgcaa ctggagccga
      481 ccacgtttgt ggatccggat cttcgccagt ttgcctgtga tgttctgtgg agtatgaaaa
      541 ccacagatgg cagggatgga tacatttata cgct
//
LOCUS       DAZFFI010000500          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFFI010000500 DAZFFI010000000
VERSION     DAZFFI010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.504"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..>572
                     /locus_tag="ACHJ12_005551"
     CDS             178..>572
                     /locus_tag="ACHJ12_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO4993141.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACML"
BASE COUNT          169 a          128 c          110 g          165 t
ORIGIN      
        1 ccaccaccca ataatttcat attgcattgt ttttcaatga aaaaatatta ttcgcgtaat
       61 atctcacgat aaataacatt aggattttgt tatttaaaca cgagtccttt gcacttgctt
      121 actttatcga taaatcctac ttttttaatg cgatccaatc attttaagga gtttaaaatg
      181 gataagaagc aagtaacgga tttaaggtcg gaactactcg attcacgttt tggtgcgaag
      241 tctatttcca ctatcgcaga atcaaaacgt tttccgctgc acgaaatgcg cgacgatgtc
      301 gcattccaga ttatcaatga cgaattatat cttgatggca acgctcgtca gaacctggcc
      361 actttctgcc agacctggga cgacgaaaat gtccacaaat tgatggattt atccattaac
      421 aaaaactgga tcgacaaaga agaatatccg caatccgcag ccatcgacct gcgttgcgta
      481 aatatggttg ccgatctgtg gcatgcgcct gcgccgaaaa atggtcaggc cgttggcacc
      541 aacaccattg gttcttccga ggcctgtatg ct
//
LOCUS       DAZFFI010000501          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFFI010000501 DAZFFI010000000
VERSION     DAZFFI010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.505"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..>570
                     /locus_tag="ACHJ12_005552"
     CDS             38..>570
                     /locus_tag="ACHJ12_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO4993142.1"
                     /translation="MKKRFILSGLTVCLLMAGCQGTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVP"
BASE COUNT          116 a          170 c          163 g          121 t
ORIGIN      
        1 ttgacctctg atttatcact gcagaggtaa atacccgatg aaaaaacgct ttatcctgtc
       61 cggactgaca gtctgcctcc tgatggcggg atgtcagggc actcagaaac tacagcaacg
      121 ggatgcggcc atgccggtac cgtcggtcac agtaaaccgt aatgtacagc ctgccagccc
      181 ggatgtgtat tctcaggcac cggaggtggt gcgctatgac cgttacctgc tggtcagcac
      241 agagccgcag gctgtacagc gtgatccact ttctcaggtg atcgacattc gcattccctc
      301 ctccctgaaa cccacggtgg ccgatgccat gcgttatgcc ctgaaacagt ccggttacac
      361 gctctgcgcc accggaccgg ccaacggcgt gctgtaccgt cagcctctgc cggcggtgca
      421 gtatcagcca ggaccactga gactgcgtac tgccctgcag ttaatggcgg ggccggcatg
      481 gcaactggaa gtggatgatg tgcagagggt ggtctgccac agtctgcgtg acggttacca
      541 gttaccggtc agccagctgg ctcctgttcc
//
LOCUS       DAZFFI010000502          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFFI010000502 DAZFFI010000000
VERSION     DAZFFI010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.506"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..237)
                     /locus_tag="ACHJ12_005553"
     CDS             complement(<1..237)
                     /locus_tag="ACHJ12_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993143.1"
                     /translation="MSSSVKYSPVPAVLTRPESLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNR"
     gene            complement(222..>556)
                     /locus_tag="ACHJ12_005554"
     CDS             complement(222..>556)
                     /locus_tag="ACHJ12_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993144.1"
                     /translation="SRRLRASLETALIVLQKWTVCVGCGYSGVRDGAGKNAPVWVKVN
                     PESGLGLCSGCDSFLLQERQRRQRHDQLFSCGLSCKQRKTEGAVYLRGSDAPGGLRDM
                     EVKVCLPQ"
BASE COUNT          139 a          182 c          138 g           97 t
ORIGIN      
        1 ccggttactg acggtcatca ctgtctccgg gcgggaaccg tcctgaaaaa catgtccggt
       61 gcgtggagaa ctgtatgaaa cataaccacc gtgcaccgca atgaccggac gacctgacgc
      121 cggaagcccc acacgacacc cactggcaat ccgggtcatc agcaggttca gttcagccag
      181 cgattcaggg cgggttaaga ccgccggaac cggagaatat ttcactgagg aagacatact
      241 ttcacctcca tatctctcag cccgccaggt gcgtcacttc cccgcagata taccgccccc
      301 tccgtctttc gctgtttgca ggaaagacca catgaaaaaa gctggtcatg ccgctgacga
      361 cgctggcgtt cctgcaaaag aaaggagtca caaccggaac acagccccag accactctca
      421 ggattcacct tcacccagac cggagcattc ttaccggcac catcccgtac cccggaataa
      481 ccacagccca cgcacaccgt ccacttctgc agtacgatga gtgcagtttc gagtgatgcg
      541 cggagacgcc gggagg
//
LOCUS       DAZFFI010000503          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFFI010000503 DAZFFI010000000
VERSION     DAZFFI010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.507"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..152)
                     /locus_tag="ACHJ12_005555"
     CDS             complement(<1..152)
                     /locus_tag="ACHJ12_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993145.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPV"
     gene            complement(154..498)
                     /locus_tag="ACHJ12_005556"
                     /pseudo
     CDS             complement(154..498)
                     /locus_tag="ACHJ12_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
BASE COUNT          119 a          199 c          120 g          116 t
ORIGIN      
        1 accggtgccc gtgacaccac ttcttctttc agcacattcg cacccgcacg ggttgcctca
       61 cgcagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct gatgtcctcc
      121 agccccgaaa aatccagcag ggtttcgatc attttcccct cccagccgac agagaatttc
      181 cagacgtccg ccggtctcat ccggcacggg cagcccgaca acgttcagga tccggtcacg
      241 ccatggacca ctcagcacat gaagtcgtga cgctgccgtg atttcccgac cggactgacc
      301 gcgcacccag atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg
      361 gctgctcctg ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat
      421 ttctccggca gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg
      481 acctgcagat attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat
      541 cagcacggaa acag
//
LOCUS       DAZFFI010000504          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFFI010000504 DAZFFI010000000
VERSION     DAZFFI010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.508"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>544
                     /locus_tag="ACHJ12_005557"
     CDS             <1..>544
                     /locus_tag="ACHJ12_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4993146.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHS
                     MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTK
                     EAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLE
                     TCKMKCSGNNIGRFIRFVFTG"
BASE COUNT          170 a           86 c          141 g          147 t
ORIGIN      
        1 ggggaggtat atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta
       61 tccgttgaat ccgatctcaa atatagatcc attaggatta gaaacactaa aatgcattaa
      121 gccactgcat tcaatgggcg gaactggtga aagaagcggt ccagatatat gggggaatcc
      181 gttctatcat caatatcttt gtgtcccaga tggtaaaggg gactatactt gtggtggcca
      241 agaccaacgg ggagaatcaa aaggagatgg tctatggggg ccaggtaaag caagtaatga
      301 tacaaaagaa gctgctggcc gttgtgacct cgttgaaacc gataatagtt gtgtggagaa
      361 ctgtttaaaa gggaagttta aagaggtaag gccgcgttat tctgtattgc ctgatatatt
      421 cacacctata aatttagggc tatttaaaaa ctgccaagac tggtctaatg attctttaga
      481 aacatgtaag atgaagtgct ccggaaataa cattggacgt tttattagat ttgtattcac
      541 cgga
//
LOCUS       DAZFFI010000505          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFFI010000505 DAZFFI010000000
VERSION     DAZFFI010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.509"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..96
                     /locus_tag="ACHJ12_005558"
     CDS             <1..96
                     /locus_tag="ACHJ12_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFO4993147.1"
                     /translation="PFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            297..>536
                     /locus_tag="ACHJ12_005559"
     CDS             297..>536
                     /locus_tag="ACHJ12_005559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993148.1"
                     /translation="MWWLVTAPVRELARNALLMAVWRVSLHKTLLYYVYQRVTRLEQP
                     VQKCPRSEHGKFSPYNGFLTPLLFSFSHINDGVYSD"
BASE COUNT          136 a          131 c          122 g          147 t
ORIGIN      
        1 ccctttgtga aggcattaac gtccaatgcc cttgttgggt tatcaacctg cgccacatcg
       61 acagaatgtt ttggccccga caggaaaaag aactaataaa tgatgctcct gcccagatat
      121 atactgggcg ggagcatact ttggttgcga cagaaatatg tcaacaccac gaactttgac
      181 attacgtctt atatcacact ggcactgaca agccagtatt gctaatgcag gaggatgctt
      241 tttcaactcg caggagtttc aggagctgcc tgatggcgct gaaatgatgc ttactcatgt
      301 ggtggctggt cactgcccct gtccgcgagc tggcacgcaa tgcactactg atggcagtct
      361 ggcgcgtaag cctgcataaa acattattgt actatgtgta tcaacgggtt acccggttag
      421 aacagccagt acaaaaatgc ccccgcagtg agcatggcaa gttttcccca tacaatgggt
      481 ttctcacccc tcttttgttt tctttttctc atataaatga tggtgtttac tcagac
//
LOCUS       DAZFFI010000506          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFFI010000506 DAZFFI010000000
VERSION     DAZFFI010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.510"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..449
                     /locus_tag="ACHJ12_005560"
     CDS             <1..449
                     /locus_tag="ACHJ12_005560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993149.1"
                     /translation="LGYFLYYGATGTIGYFLYSGATGTIGYFLYYGATGTIGYFLYSG
                     ATGTIGYFLYSGATGTIGYFLYSGATGTIGYFLYYGATGTIGYFLYYGATGTIGYFLY
                     SGATGTIGYFLTLVPLAPLAIFCTLVPLAPLTIFCTLLPLATPLAF"
BASE COUNT           99 a          125 c          107 g          201 t
ORIGIN      
        1 cattgggtta ttttttatac tatggtgcca ctggcaccat tggctatttt ttgtactctg
       61 gtgccactgg caccattggt tattttttat actatggtgc cactggcacc attggctatt
      121 ttttgtactc tggtgccact ggcaccattg gctatttttt gtactctggt gctactggca
      181 ccattggcta ttttttgtac tctggtgcca ctggcaccat tggctatttt ttatactatg
      241 gtgccactgg caccattggt tattttttat actatggtgc cactggcacc attggctatt
      301 ttttgtactc tggtgccact ggcaccattg gctatttttt gactctggtg ccactggcac
      361 cattggctat tttttgtact ctggtgccac tggcaccatt gactattttc tgtactctgt
      421 tgccactggc aacaccgctc gctttttaat cactgttgcc actggcaaca tggttaatga
      481 tgactactac cttaacgttc agagacaaaa acagcattaa tgttgttatc cc
//
LOCUS       DAZFFI010000507          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFFI010000507 DAZFFI010000000
VERSION     DAZFFI010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.511"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..>528)
                     /locus_tag="ACHJ12_005561"
     CDS             complement(5..>528)
                     /locus_tag="ACHJ12_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4993150.1"
                     /translation="QMNEGWYQIRIAGEDTAPEAVYARLHEQLGEGAVIHIMPRLAGA
                     GGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGDGKVVVIGRG"
BASE COUNT          111 a          182 c          118 g          117 t
ORIGIN      
        1 atttttatcc ccgcccgata accaccactt tcccgtctcc gccctcatca cgggtgctga
       61 tatcctggga tatccgtcgt gaaccaacca gcatttcacc gtaaggcacc ggcatcgggt
      121 taccctgggc aatcatgttg tccagtgacg aaaaatacgt gttctgttta ccgttatccg
      181 tacttttata ctccggcgtc tttgccttcg gggccagcat ctgagccaca ccacccagta
      241 tcatgctggc acccagtgag aacagcatcg tggtggcagt cagccctccg gcactcaggg
      301 ctgcgcccca tgccgccagt gttgcaccac cagtgaaaaa tgacccgaca atcgctgcaa
      361 cccccagcac cacctgaaaa acaccatttc ccccggctcc ggccagtcgc ggcataatgt
      421 ggatgaccgc tccctcaccc agttgttcgt gaagacgggc gtacaccgcc tccggtgcgg
      481 tatcctcacc ggcaatacgt atctgatacc agccttcgtt catctgac
//
LOCUS       DAZFFI010000508          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFFI010000508 DAZFFI010000000
VERSION     DAZFFI010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.512"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>523
                     /locus_tag="ACHJ12_005562"
     CDS             <1..>523
                     /locus_tag="ACHJ12_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4993151.1"
                     /translation="LSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNVRVISKSN
                     EVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAMEPE
                     QKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEVNAAQK
                     LVNAGNQRLKILT"
BASE COUNT          158 a           99 c          108 g          158 t
ORIGIN      
        1 tcttatcacg gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata
       61 tgactgttta tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa
      121 tatctaaaag taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat
      181 cgacgcctca ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta
      241 tgccacctaa atttgcttta ccagccggta gtagcaaaac cgtacgtttt gttgcgatgg
      301 aaccagagca aaaagagaaa aattatcgcg ttaaatttga agcggttccc agtattgatg
      361 acgttgccac agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga
      421 ttgttgttag tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa
      481 aattagttaa tgcagggaat caacgcttaa agattttaac gat
//
LOCUS       DAZFFI010000509          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFFI010000509 DAZFFI010000000
VERSION     DAZFFI010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.513"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..519
                     /locus_tag="ACHJ12_005563"
                     /pseudo
     CDS             154..519
                     /locus_tag="ACHJ12_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          139 a          142 c          133 g          108 t
ORIGIN      
        1 cacggcgcgt tcgtcttcat gaaacaccgg ccgcctggtg ccacacacgc cgggaatgct
       61 acagcaaaac gaccggtcgt cgctgcggca gccctgacag tctcagcagg ctgattctga
      121 gcagtattca gcccatagaa tgagataacc cgtatgaatg atgacagaca gacgtctacg
      181 gtacccgatg tatggaacat ttgctttctg gctaacggca tgcccgacac atggttttac
      241 accgatgacg cctccgtcac aaaagagtgg gggcgggaat attgtgacaa tgtggaagtg
      301 ttccctctgt gctatatgca tgacggtccc gttttccggg ggcgctatat tgatgagctc
      361 agcgccgcac ggaacagaat gctccatatg caccacatac tgccggaatg aacccggtac
      421 agagaaaatt ttcatgacgc agaatgtatc cgtgaaattc acgaagcgcc cctgcaggct
      481 cccggcattc tgcaaaaata caaacgcaca aaatcataag gc
//
LOCUS       DAZFFI010000510          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFFI010000510 DAZFFI010000000
VERSION     DAZFFI010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.514"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            154..519
                     /locus_tag="ACHJ12_005564"
     CDS             154..519
                     /locus_tag="ACHJ12_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993152.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAPGILEKYKRTKS"
BASE COUNT          137 a          152 c          134 g           99 t
ORIGIN      
        1 cgcggcgcgt tcgtcttcat gaaactccgg ccgcctggtg ccacacgcac cgggaatgct
       61 acagcaaaac gaccggtcgc cgctgcggca gccctgacag tctcagcagg ctgattctga
      121 gcagcattca gcccatagaa tgagatgacc cgtatgaatg atggcagaca gacgcctccg
      181 tcccctgagg catggaaaat ccggtttctg gcgaacggta cacccggcac atggttttac
      241 accgatgacg cagccgtcac aaaagagtgg ctgcgggcac attgtgacga tgtggaagtg
      301 atccccctgt gccgtatgca tgacggtccc gttttccggg aacaccatat taatgaactc
      361 agcgccgcac tggccagaat gctccatatg caccacatac tgctggaacg aacccggtac
      421 agagaaaata tccatgatgc agaatgtatt cgtgaaattc acgaagctcc cctgcaggct
      481 cccggcattc tggaaaaata caaacgcaca aaatcataag gc
//
LOCUS       DAZFFI010000511          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFFI010000511 DAZFFI010000000
VERSION     DAZFFI010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.515"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>515
                     /locus_tag="ACHJ12_005565"
     rRNA            <1..>515
                     /locus_tag="ACHJ12_005565"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          108 c          161 g          106 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccga
//
LOCUS       DAZFFI010000512          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFFI010000512 DAZFFI010000000
VERSION     DAZFFI010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.516"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>515)
                     /locus_tag="ACHJ12_005566"
     CDS             complement(<1..>515)
                     /locus_tag="ACHJ12_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO4993153.1"
                     /translation="ASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRT
                     DEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRW"
BASE COUNT          117 a          155 c          123 g          120 t
ORIGIN      
        1 gccaccgatt gtaatcacat tcgatataat taaacaccgt tgcccgcatt atttcccggc
       61 tgataaagtg ttctccatgg atacattcca ctttcagcga atgaaagaag ctttccacgc
      121 aggcattatc gtagcagcaa ccttttgcgc tcatacttcc acgcagatta tgccgcttca
      181 gttgcgcctg ataatctgct gaacagtact ggcctccacg gtccgtgtga acgataacgt
      241 tccggggcct cttacgccgc cacagcgcca tctgcagggc atcgcaggcc agttgcgccg
      301 tcatgcgtgg cgacattgac cagccaataa cggcacgtga ccacaggtca atgaccactg
      361 ccagatacag ccagccttca tctgtacgta agtacgtgat gtctcctgcc cacttctggt
      421 tcgggccact ggcgtaaaaa tcctgctcca acagattttc tgacacaggc aggccgtgtg
      481 cgcggtagct gaccgggctg aacttccggg aggcc
//
LOCUS       DAZFFI010000513          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFFI010000513 DAZFFI010000000
VERSION     DAZFFI010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.517"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..482)
                     /locus_tag="ACHJ12_005567"
     CDS             complement(<1..482)
                     /locus_tag="ACHJ12_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005285503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993154.1"
                     /translation="MKNANRVVINLFSISLVTMISGCASYWGKVPYNPPSDTEKNIAH
                     VRLIGNPPGFTISQYGNKPAAVDPSNMVILRPTHDLGFPKVNNDPERYKETYYDTNVY
                     AGTPTRIGFKFLHGCEDVGYTFLPEKGETYEFRLSVTDKTGYCVLYASHLKYDSENKL
                     "
BASE COUNT          157 a          105 c           89 g          162 t
ORIGIN      
        1 tacaatttat tttctgaatc atatttcagg tgggatgcat agagaacgca ataccctgtt
       61 ttatcagtga cagacaatcg aaattcgtag gtctcgccct tttccggtaa aaaagtataa
      121 cctacatctt cacagccatg aagaaacttg aaaccaatcc gtgtcggagt accagcatag
      181 acatttgtat cataatatgt ctctttataa cgttcgggat cgttatttac ttttggaaaa
      241 cctaaatcgt gtgttggtct taaaatcacc atattagaag gatcaaccgc agcaggctta
      301 tttccatact ggcttattgt gaaacctggt ggattaccga ttaatctgac atgcgctatg
      361 ttcttctctg tatctgacgg tggattatag ggaactttcc cccaataaga agcacaccca
      421 gatatcatgg tcacaagaga tatactaaaa agattaatta ctactcgatt ggcgtttttc
      481 atagcaacat ccatcaaaca tcatctttat ttt
//
LOCUS       DAZFFI010000514          510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFFI010000514 DAZFFI010000000
VERSION     DAZFFI010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.518"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>510)
                     /locus_tag="ACHJ12_005568"
     CDS             complement(<1..>510)
                     /locus_tag="ACHJ12_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4993155.1"
                     /translation="TTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDFV
                     AAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFWM
                     EGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSEL
                     LEQRAEPFPR"
BASE COUNT           96 a          166 c          134 g          114 t
ORIGIN      
        1 ccggggaaac ggctcggcac gctgttcaag cagttcactt ttacgctgtt ccgtggctga
       61 acggggcaga tagggtcgtc cgatatcggt gttcaccacc gctttcaggg tctcttcact
      121 gccggttcgc tcatactctt cttctgccgc cagcagttta aaaatcagtt gttcccaggt
      181 ctgaaacgcc gcagccggcc cctccatcca aaatgacgca atccgggaat ttcgtggcgt
      241 tccggtgata ctgccgtccg ccgccgcccg ttcaccttca cgaagccaga tcccctggtt
      301 attcagttca cgcttctgtt ccggagcaat caggccgcgg cagtgcggac acatcaggcg
      361 ggccgcctgc ccggcagcca caaaatcagg gttattccgg taaccggtca tgttatccat
      421 caccggctga aaatattccc cgcagtgcgg acacggccag taccaccggc ggcggtctcc
      481 ccggttatac agtgacagga tccccgttgt
//
LOCUS       DAZFFI010000515          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFFI010000515 DAZFFI010000000
VERSION     DAZFFI010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.519"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          170 a           95 c           97 g          143 t
ORIGIN      
        1 agaattggta ataatgaaag ttaataacat aaaaaagagg ttaccgcctt ataatgataa
       61 gagttcatta tccattccat cccgacaaaa cctgcctgtg tataaaaaat aaggaccatg
      121 tcacaaaaca ttacattccg gcaagttatg acagaattac tgaaaaccgt tatctgtagt
      181 aattgtagtt tctgatatat gctcatccat ccggaaatac gacatggtcc tgactggcgt
      241 tcacttcaaa gtgtaattta ttattcacac actgagactg aaaaacggat ggcgtggatg
      301 agtacacagt ctgaagagta taaaaggaac agctccggat tgttacccgg gattactata
      361 gttcaccaca caaccgtaaa cctggttgtg tggtgaaaca gtaacatatt aagaatattg
      421 tgttaccacc tgttttctca ggaaacggat tgcaggagag gtatactcat tcatcttccc
      481 ggagttttgt taatcactgc ataat
//
LOCUS       DAZFFI010000516          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFFI010000516 DAZFFI010000000
VERSION     DAZFFI010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.520"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>503)
                     /locus_tag="ACHJ12_005569"
     CDS             complement(<1..>503)
                     /locus_tag="ACHJ12_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO4993156.1"
                     /translation="AYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAPHRAIFRAMS
                     ELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYAGIVVEKSV
                     LRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASLSSALDTVL
                     QQLESVLG"
BASE COUNT          113 a          156 c          130 g          104 t
ORIGIN      
        1 ccgagcacac tttccagttg ctgcagaacg gtatcaagcg cggatgaaag agaggcttcc
       61 gtgcgttccc ggagcgtcct gttctgcgca agtgcaaaca gacgccgctc tgcagcatcc
      121 agcacatccc gtgatgtggc atttggtgcc gccacatcgg caatcagggt gtttccggtg
      181 gcctgtaact gacgcagaac gcttttctcc accacaatgc ctgcataggc catgatgttg
      241 gccgcagaag gtgtgttctt gctgagctcg gccagataag caaaaccacc cagtacgtcc
      301 agcctgccct gatgttccag atgctctgac agagtaatca ggtccagagg ctggccattc
      361 accgccagtt cagacatggc acggaagatg gcccggtgcg gagccacata aaaatcgtca
      421 ggactcagca tcagcaccac ttcatcccag cgttcattct ccagcatcag cccacccaga
      481 acggcctgct ctgcttcgta tgc
//
LOCUS       DAZFFI010000517          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFFI010000517 DAZFFI010000000
VERSION     DAZFFI010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.521"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>503
                     /gene="subA"
                     /locus_tag="ACHJ12_005570"
     CDS             <1..>503
                     /gene="subA"
                     /locus_tag="ACHJ12_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFO4993157.1"
                     /translation="SNVFLAPGEEKIINISGGQKGISSASVWSELLSRMGRNNERLIV
                     AAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRVAALAQYRKGETPVLHGGG
                     VTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATAIVSGVLAAMVSCNPRATA
                     TELKRTL"
BASE COUNT          139 a          105 c          149 g          110 t
ORIGIN      
        1 tgtcaaatgt ttttcttgca ccaggagaag agaaaatcat taatatatcg ggaggccaga
       61 aggggatatc ttccgcatcg gtctggtccg aactgctttc ccgtatgggc cggaataatg
      121 agcgattaat tgttgcggca gtgggtaatg atggcgcaga tatacgaaaa ctgagtgctc
      181 agcagagaat atggcctgcg gcttaccatc cggtcagcag tgtgaataaa aagcaggatc
      241 ctgtgataag agtggcagct ctggcacagt accggaaagg agaaacacct gtattgcatg
      301 gtggaggggt caccggaagt cggttcggaa acggctgggt tgatattgcc gcaccgggac
      361 agaatattac attcctcaga ccagatggaa aaacggggat cggtagtgga acctcggaag
      421 caacagctat agtttccggc gttctggcag cgatggtctc atgtaatccc cgtgcaacag
      481 cgacagaact gaagcgtaca ctg
//
LOCUS       DAZFFI010000518          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFFI010000518 DAZFFI010000000
VERSION     DAZFFI010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.522"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>496)
                     /locus_tag="ACHJ12_005571"
     CDS             complement(<1..>496)
                     /locus_tag="ACHJ12_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993158.1"
                     /translation="DDIRTNKIIKNHLRIMEKYTRESCEKRFIYFLGSRKKVRFSTKK
                     QPRYNPFTKNIVFTLLIGKEERKLRIKYSFCTFTQNGVVKVTPNVRVTEKFLYIEYEN
                     GNLCVMSVHDFVNGHYIDLGIHTEVHYVGYTRHPHRRPVDFIHRGLSKMLYSVSNDDN
                     DFFVC"
BASE COUNT          151 a           99 c           71 g          175 t
ORIGIN      
        1 acaaacaaaa aaatcattat cgtcatttga aacgctatac agcatctttg ataagccacg
       61 gtgaataaaa tcaacagggc gccgatgcgg atgacgtgta tatcctacgt aatgcacttc
      121 tgtatgaatt cctaaatcaa tataatgtcc gtttacgaag tcatgaactg acattacaca
      181 taaattgcca ttttcatatt caatgtagag aaacttctca gttactctaa catttggagt
      241 taccttcact accccattct gcgtaaacgt gcaaaaggaa tattttatcc gaagctttcg
      301 ctcttctttg ccaatcaaca gagtgaatac tatatttttt gtgaatgggt tatacctagg
      361 ctgtttcttg gttgaaaacc tcactttttt tcttgaacct aagaaataaa taaatctctt
      421 ttcacagctt tccctcgtat atttttccat aattctgaga tggttcttta ttatcttatt
      481 tgttcttata tcatca
//
LOCUS       DAZFFI010000519          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFFI010000519 DAZFFI010000000
VERSION     DAZFFI010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.523"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(17..331)
                     /locus_tag="ACHJ12_005572"
     CDS             complement(17..331)
                     /locus_tag="ACHJ12_005572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993159.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSPVYFRDAVAFSSLALFDPESVASCDATG
                     LI"
BASE COUNT          141 a          126 c          125 g           98 t
ORIGIN      
        1 acactctgca tctgaatcaa attaatccgg ttgcatcaca ggaagccact gattcggggt
       61 caaacagcgc cagactggaa aaagccaccg catccctgaa gtacaccggc gatggttcat
      121 ctgtaataag ttgtctgatc agacattccg ccagcacgga catctgtggc cggatctgta
      181 aaagcaaaga cgacgggctg actgccatac cggataatgg cgtgcagaac cgcagatacc
      241 gcctcaccac ctcctcactg aggtcagcga taaggaaacg tccggtatga gcctgaaaag
      301 cagggcgcag atgcccggca atcagaagca ttgtgccggc aggtatgaca gagttcctgt
      361 gctccagaca aagcattgtc tgtttgagag ccataatgac agcgcaggta cagggcatcg
      421 gatatccggt aaatatgctg atttattaaa gagacagaca tgccccgtat ataaaattcc
      481 gtgtcaccgg
//
LOCUS       DAZFFI010000520          489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFFI010000520 DAZFFI010000000
VERSION     DAZFFI010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.524"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..302
                     /locus_tag="ACHJ12_005573"
     CDS             <1..302
                     /locus_tag="ACHJ12_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993160.1"
                     /translation="GDTGGRLALFVKAKECGTCDARLASVLATGRQVDIYLVDSQGKD
                     EILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRFGEGKMPVVLQQGADGWRVAAF"
     gene            281..>489
                     /locus_tag="ACHJ12_005574"
     CDS             281..>489
                     /locus_tag="ACHJ12_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFO4993161.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRD"
BASE COUNT           90 a          119 c          169 g          111 t
ORIGIN      
        1 ccggagatac ggggggacgg ctggcgctgt ttgtgaaggc gaaggaatgt ggcacctgtg
       61 atgcccgtct tgcctccgta ctggccacgg gacggcaggt tgatatctac cttgtcgaca
      121 gtcagggaaa ggatgagatt ctgcgtcagt gggcgcatgc gcacagcata cctgtggaga
      181 aagttcgcag ccgtcatatc accctgaatc atgatgccgg acgctggctg cgttttggtg
      241 aaggaaaaat gccggtggta ctgcagcagg gggctgacgg atggcgcgtg gcggcattct
      301 gacggttctg ctgatggtgg cctgcagtgg tgtctgtcgt gctgccgttc agaacgtacc
      361 ggacgggtac gtgcgggtgg cccgtgccca tggtattccc gcagagcttt tgtatgccgt
      421 ttccctgacg gaaacgtcca tgtctccgga agcgatgtcc ggagtactga aacaacggcg
      481 tcgtgattt
//
LOCUS       DAZFFI010000521          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFFI010000521 DAZFFI010000000
VERSION     DAZFFI010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.525"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>485
                     /locus_tag="ACHJ12_005575"
     rRNA            <1..>485
                     /locus_tag="ACHJ12_005575"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZFFI010000522          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFFI010000522 DAZFFI010000000
VERSION     DAZFFI010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.526"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..395)
                     /locus_tag="ACHJ12_005576"
     CDS             complement(<1..395)
                     /locus_tag="ACHJ12_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO4993162.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACML"
BASE COUNT          133 a          106 c          114 g          132 t
ORIGIN      
        1 agcatacagg cctcggaaga accaatggtg ttggtgccaa cggcctgacc atttttcggc
       61 gcaggcgcat gccacagatc ggcaaccata tttacgcaac gcaggtcgat ggctgcggat
      121 tgcggatatt cttctttgtc gatccagttt ttattgatcg acaaatccat caatttatgg
      181 acgttttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc gttgccatca
      241 agatataatt catcattgat aatctggaat gcgacatcat cgcgcatttc gtgcagcgga
      301 aatcgttttg actccgcgat agtagaaatg gcctttgcgc caaaacgtga atcgagtagt
      361 tctgagcgga aatccgttaa cagcttctgg tccatttcga actccttaaa tttatttgaa
      421 ggcaataaaa aagtaggatt tatccgcaat ggaagcaagg cattacaggc ttaatttaaa
      481 taaca
//
LOCUS       DAZFFI010000523          477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFFI010000523 DAZFFI010000000
VERSION     DAZFFI010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.527"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..391
                     /locus_tag="ACHJ12_005577"
     CDS             <1..391
                     /locus_tag="ACHJ12_005577"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4993163.1"
                     /translation="LNKIVADYNLSDYVEFMGFRHDLDQIRLDNDVCLIPSLSEAFGR
                     VTIESMAAGMIVVASDSGASKEIINDGINGFLFSSGSVSDLTSVLKKILDVESNNLEC
                     IRKRALVDSQKYTSGHAASSIYNLIIN"
BASE COUNT          152 a           55 c           93 g          177 t
ORIGIN      
        1 tcttaataaa attgttgctg attataattt gagtgattat gtagagttta tgggatttag
       61 gcatgatctt gatcaaataa ggcttgacaa cgatgtttgc ttaattccct ctctttctga
      121 agcttttggt agagttacca ttgaatcaat ggctgcaggt atgatagttg ttgctagtga
      181 ttctggtgct agtaaagaga tcatcaatga tggtataaac ggttttttgt tttcttcagg
      241 ttcggtaagt gatcttacta gcgtacttaa aaaaatcctt gatgtagagt ctaataattt
      301 agaatgtata aggaaaaggg ctttggttga ctcacaaaaa tatacatcag gacatgcagc
      361 ttcatcaatt tataatttga tcattaactg attttttata aactttgatt atcattttta
      421 aatgttaatt aaaaaaacaa taagggctaa ttagtgcaaa gtaatgttaa agggttg
//
LOCUS       DAZFFI010000524          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFFI010000524 DAZFFI010000000
VERSION     DAZFFI010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.528"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>476
                     /locus_tag="ACHJ12_005578"
     rRNA            <1..>476
                     /locus_tag="ACHJ12_005578"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFFI010000525          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFFI010000525 DAZFFI010000000
VERSION     DAZFFI010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.529"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..336
                     /locus_tag="ACHJ12_005579"
     CDS             49..336
                     /locus_tag="ACHJ12_005579"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4993164.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            349..>473
                     /locus_tag="ACHJ12_005580"
     CDS             349..>473
                     /locus_tag="ACHJ12_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFO4993165.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTV"
BASE COUNT          125 a           96 c          126 g          126 t
ORIGIN      
        1 caggacattt tgtctgttat tgcgcgctat taatcctgag gaacatttat gaaaattaca
       61 gtggtttgcg gtaacggctt aggcaccagc ctgatgatgg aaatgagcat taaaagcatt
      121 ttgaaggatt tgtccgtcag cgctgatgtt gatcatgtcg acctgggctc cgcgaaaggg
      181 acgccgagtg atatctatgt cggcaccaaa gacattgccg agcagttagt cgcgcagtct
      241 gtcgcaggca aaatcgtcgc actggaaaat atgattgata agaaagccat gagagaacgt
      301 ttatccgtgg cattaaccga attaggcgca ctgtaagctg ggggcgatat ggatttcttt
      361 cgttttttga tgagcgatgt actttccgaa cccgcagtgc tggtcggttt aatcgccctg
      421 attggcctga ttgcacagaa aaaaccggta accgaatgca ttaaaggtac cgt
//
LOCUS       DAZFFI010000526          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFFI010000526 DAZFFI010000000
VERSION     DAZFFI010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.530"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>471)
                     /locus_tag="ACHJ12_005581"
     CDS             complement(<1..>471)
                     /locus_tag="ACHJ12_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993166.1"
                     /translation="SSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQTSIQ
                     MVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSDNTCE
                     LTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRNI"
BASE COUNT          147 a           80 c           82 g          162 t
ORIGIN      
        1 gatattccgt aagtccagag aggatagatc aactatacct ccagtcccac aactaaaaac
       61 taagttcaac ctcctgattt cattgtaaat gtctttcaat gggaaattaa cattgatatt
      121 gcctgtggat atttcctggt tttttaacgg ttcattaatt gtaagttcac aggtattgtc
      181 tgatcgttga ataattaggg cgttataacc aaaaaaatcc atacgaagag acgaatcatt
      241 ataaataatt acattttgta agtacgctaa ttctttttga aaatcaccag tgataatgca
      301 ttttccacct ggttgtaacg cgttaagtaa agtgaccatt tgaatagagg tctgttttag
      361 tttttctata cctggtgcta gtgcttcgag gttattaact atggggcttt caggaagatc
      421 attcattttt cgacggacta aatcactact aatatcatta ccaattgatg a
//
LOCUS       DAZFFI010000527          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFFI010000527 DAZFFI010000000
VERSION     DAZFFI010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.531"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>469)
                     /locus_tag="ACHJ12_005582"
     CDS             complement(<1..>469)
                     /locus_tag="ACHJ12_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993167.1"
                     /translation="WERIPLPVTLMAGQERVIFADKNVRVGFPPALNGKLRVQSTGGA
                     VYLKAESVFPQTRLQLQDVESGEIILLDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAA
                     SARSAEHKKVVPGGDDGSQALRQPVKYAEPLPVLLTRYAAQSLYAPLRTLEAVP"
BASE COUNT           92 a          153 c          115 g          109 t
ORIGIN      
        1 ggtaccgctt ccagcgtccg taacggtgcg tacagcgact gtgccgcata gcgggtcaac
       61 agaacgggta aaggctctgc atacttcacc ggctgacgga gggcctgcga gccgtcatcc
      121 ccgccgggta ccaccttttt gtgttctgca gagcgtgcag acgccgctgt gccagcctga
      181 ctgaccgtac tgacgcttcc ctcatacaca atccgtacgg gctctgtggg ccctttttct
      241 gctgctgtca catccagcag aataatttct ccgctctcca catcctgcag ttgcagacgc
      301 gtctgcggaa aaacactctc tgctttcaga tagactgccc caccggtact ctgtaccctc
      361 aactttccgt tcagtgctgg cgggaagccc acccgcacat ttttatctgc aaaaataacc
      421 cgctcctgtc ctgccatcag ggtaacgggt aagggaatgc gctcccatt
//
LOCUS       DAZFFI010000528          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFFI010000528 DAZFFI010000000
VERSION     DAZFFI010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.532"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          161 a           86 c           82 g          140 t
ORIGIN      
        1 tgagtgctga atctatttta aatggtatca taaatacctg tccacactta agattttcct
       61 gcggaaaaca aatattaaca tattttatgt attccgcatg gtgatgattt atacacctgc
      121 actgaatttt tctgtatctc cagagctgaa tcgataaaca gaagtggtaa taatgaaagt
      181 taatcacata aaaaagaggt taccgcctta taatgataag agttcattat ccattccatc
      241 cagacaaaac ctgcctgtgt ataaaaaata agaaccatgt cacaaaaaca ttgcattccg
      301 gcaagttatg acagaattac tgaaaaccgt tatctgtagt tattgtagtt tctgatatat
      361 gctcatccac ccggaaatac gacatggtcc tgactggcgt tcagttcaaa gtgtaattta
      421 ttattcacac actgagactg aaaaacggat ggcgtggatg agtacacag
//
LOCUS       DAZFFI010000529          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFFI010000529 DAZFFI010000000
VERSION     DAZFFI010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.533"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>468)
                     /locus_tag="ACHJ12_005583"
     CDS             complement(<1..>468)
                     /locus_tag="ACHJ12_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HFO4993168.1"
                     /translation="AALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPL"
BASE COUNT           77 a          162 c          150 g           79 t
ORIGIN      
        1 agcggcgcgg caggtaaatt ctccgggtat tccgggtcgt gcgtcagcca cacggcagac
       61 agacggatgc cgttgtcccg gccatattcg gtgtaacccg gcagggtatc aggaaaagcg
      121 gacggttccg tcccgccgga aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc
      181 gtggtcagta ccagccggaa gtgacgcccg gcaccatccg tcacgccggt gatttcgccg
      241 ctgaattcac cggcggcttc gcggtggaac gtctgtgtgc gcccgaagcg gtccaccagc
      301 ccggtcagta cccggtacgg cggcagcggc gcaggcagca cctcatccgc ttccggcacc
      361 cgctcacacc agccgagcag ccaccacggc ccctgcggac tgtttgtcgc cagataacga
      421 tgcggactta agcggagttc ttccggcagc gcctgccaga gtgcggcc
//
LOCUS       DAZFFI010000530          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFFI010000530 DAZFFI010000000
VERSION     DAZFFI010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.534"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>460
                     /gene="espX5"
                     /locus_tag="ACHJ12_005584"
     CDS             <1..>460
                     /gene="espX5"
                     /locus_tag="ACHJ12_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO4993169.1"
                     /translation="PSHTKKTKSDNYSKHHKNTLIDNKALSLFKMDDHEKVIGLIQKM
                     KRIYDSLPSGKITKETDRKIHKHFIDIALYANNKCDDRITRRVYLSKEKEVSIKVVYF
                     INNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIKDEDLSNSNFAGCRLQNA"
BASE COUNT          189 a           68 c           78 g          125 t
ORIGIN      
        1 gtccatcaca tacaaagaaa acaaaatcag ataattacag caaacatcat aaaaacacgt
       61 taattgacaa taaagccctc tctcttttca agatggatga tcatgaaaaa gtgataggtt
      121 tgattcagaa aatgaaaaga atttatgata gtttaccatc aggaaaaatc acgaaggaaa
      181 cggacaggaa aatacataaa cattttatag atatagcttt atatgcaaat aataaatgtg
      241 acgatagaat tacgagaaga gtttacctta gtaaagaaaa ggaagtatcc attaaggtgg
      301 tatattttat aaataatgtc gccgtccata ataatactat cgaaattcca cagacagtaa
      361 atggtggtta cgatttttca caccttagcc tgaaaggtat cgtgattaaa gatgaagatt
      421 tatccaattc gaattttgca ggttgcagac tacaaaacgc
//
LOCUS       DAZFFI010000531          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFFI010000531 DAZFFI010000000
VERSION     DAZFFI010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.535"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>458
                     /locus_tag="ACHJ12_005585"
     CDS             <1..>458
                     /locus_tag="ACHJ12_005585"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFO4993170.1"
                     /translation="PQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGDELK
                     VYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHADEE
                     KPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGSLITAMKNA"
BASE COUNT          128 a           97 c          117 g          116 t
ORIGIN      
        1 tccacaactg gctgaactgg aatcaggacg ggtatcccgt atctggaatg ataaaaaaat
       61 tacggctcac catgccatta ttccgacccg gcaggttttt gacctcagct cactgaacgg
      121 tgatgaactg aaggtatatc aactgattcg tcagcactat ctggcacagt ttctgccgga
      181 gcaggaagct gatgttactg aggcaacatt tgtgattggg gggcagttat tccgttcccg
      241 cggaagagtt aatgtgatta tcgggtggaa acagttattt ccaccggaaa atcatgcaga
      301 tgaagaaaag cctgacagtg atgaaagtgg aaacatattg ccccctttga taaagggaga
      361 actctgtgct ctggatggtg ctgaacagaa ggagctgaaa accgtacctc cccgtccttt
      421 tactgaaggc agtcttatta ctgcgatgaa gaatgctt
//
LOCUS       DAZFFI010000532          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFFI010000532 DAZFFI010000000
VERSION     DAZFFI010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.536"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>454)
                     /locus_tag="ACHJ12_005586"
     rRNA            complement(<1..>454)
                     /locus_tag="ACHJ12_005586"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          145 c           99 g          127 t
ORIGIN      
        1 gccttctccc gaagttacgg caccattttg cctagttcct tcacccgagt tctctcaagc
       61 gccttggtat tctctacctg accacctgtg tcggtttggg gtacgatttg atgttacctg
      121 atgcttagag gcttttcctg gaagcagggc atttgttgct tcagcaccgt agtgcctcgt
      181 catcacgcct cagccttgat tttccggatt tgcctggaaa accagcctac acgcttaaac
      241 cgggacaacc gtcgcccggc caacatagcc ttctccgtcc ccccttcgca gtaacaccaa
      301 gtacaggaat attaacctgt ttcccatcga ctacgccttt cggcctcgcc ttaggggtcg
      361 actcaccctg ccccgattaa cgttggacag gaacccttgg tcttccggcg agcgggcttt
      421 tcacccgctt tatcgttact tatgtcagca ttcg
//
LOCUS       DAZFFI010000533          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFFI010000533 DAZFFI010000000
VERSION     DAZFFI010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.537"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>454
                     /locus_tag="ACHJ12_005587"
     CDS             <1..>454
                     /locus_tag="ACHJ12_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylovoran biosynthesis protein AmsE"
                     /protein_id="HFO4993171.1"
                     /translation="DTDDICVPERFEKQISYFQKNPSTIILGGAIDEYDETFKDRRGR
                     RFSCCSHSDIVSYSLFRNPFNHMTVMFNKEFILGIGGYKHHHYMEDYNLWLRCIANGA
                     ICSNIDNTLVLARTGDAMIERRRGRDYLKSEIELARLKIKCFPNKKAK"
BASE COUNT          157 a           57 c          101 g          139 t
ORIGIN      
        1 tggatactga tgatatctgt gttcctgaaa ggtttgagaa acaaataagt tattttcaga
       61 aaaatccaag tacgattata ttaggtgggg ccattgatga atatgatgag acttttaaag
      121 ataggagagg gcgtagattt tcatgttgct cgcacagtga tatagtgagc tattctctct
      181 ttagaaatcc attcaaccat atgactgtaa tgttcaataa agaatttatc ttgggaatag
      241 gtggttacaa gcatcatcat tacatggaag attataattt atggctaagg tgcattgcta
      301 atggtgccat atgttctaat attgataata cgctggtcct tgcacgaaca ggtgatgcaa
      361 tgatagaacg aaggagggga agggattatt taaaaagtga aatcgaattg gcacgcttga
      421 aaattaagtg ttttccaaat aaaaaagcaa aaat
//
LOCUS       DAZFFI010000534          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFFI010000534 DAZFFI010000000
VERSION     DAZFFI010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.538"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     repeat_region   10..282
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10..38
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          127 a          115 c          104 g          104 t
ORIGIN      
        1 atgtttacag agttccccgc gccagcgggg ataaaccgag atttgtagaa aaatagtcat
       61 tctctgcctg gagttccccg cgccagcggg gataaaccgc cggacactac cgaacgacag
      121 cggcggcggg cgagttcccc gcgccagcgg ggataaaccg aaaccggacg agggcgcgct
      181 aaaacgtgat tagagttccc cgcgccagcg gggataaacc gcctctcaat ttgtaacgtc
      241 tgcaatgtca ggcgagttcc ccgcggcagc ggggataaac caccatataa cccgttatct
      301 ctttctcaag tttttatatt agcagtactt gtaataagca acatatccac gtaacacctc
      361 atgttcaaaa tagttctcca tgccagagag gttcacaatt atcgatacaa aaaattaaat
      421 ttaatcaaag tgttatttgt atgattctta
//
LOCUS       DAZFFI010000535          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFFI010000535 DAZFFI010000000
VERSION     DAZFFI010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.539"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>445)
                     /locus_tag="ACHJ12_005588"
     CDS             complement(<1..>445)
                     /locus_tag="ACHJ12_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4993172.1"
                     /translation="IGIVGRSGSGKSTLTKLLQRFYIPETGQILIDGHDLSLADPEWL
                     RRQIGVVLQENILLNRSIIDNITLASPAVSMEQAIEAARLAGAHDFITELKEGYNTIV
                     GEQGVGLSGGQRQRIAIARALVTNPRILIFDEATSALDYESENIIM"
BASE COUNT          132 a          101 c           79 g          133 t
ORIGIN      
        1 cattattata ttttccgact cataatcaag agcactggtt gcttcatcaa aaataagaat
       61 tcgaggattt gtaacaagag cccgggctat tgcgatccgt tggcgctgcc ctcctgaaag
      121 accaacgccc tgttctccaa caatagtatt gtacccttct tttagttctg taataaaatc
      181 atgggcacct gcaagtctgg ctgcctcaat agcctgttcc atagatacag ccggagaagc
      241 taatgtaata ttatcgataa tactacgatt taatagtata ttttcctgta atacaacacc
      301 aatctggcgt cgtaaccatt ctggatctgc aagtgataaa tcatgcccat caattaaaat
      361 ctgtccggtc tctggtatat aaaagcgctg aagcagtttt gttaatgtac tttttcctga
      421 accagaacgg ccaactatac cgata
//
LOCUS       DAZFFI010000536          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFFI010000536 DAZFFI010000000
VERSION     DAZFFI010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.540"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>445
                     /locus_tag="ACHJ12_005589"
     CDS             <1..>445
                     /locus_tag="ACHJ12_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFO4993173.1"
                     /translation="DSRMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRH
                     RLVPGLRITDVARKLNTRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPA
                     DALRAVQLLWNEMSNPGGTFPVTHDTYLKLFQLSGADLSHRWDTIL"
BASE COUNT           95 a          114 c          134 g          102 t
ORIGIN      
        1 gacagccgta tgctctatct tgcctataac cgggcaatac gtgatgaggc agagcgtaaa
       61 tttcccttta acgtggaatg cagaacgtca catcagctgg cgtatgcccg ggttggaaga
      121 cactaccgtc atcgtctggt tccgggatta cgtattactg atgtggcgag aaagctgaat
      181 acacgatact gggctctggc acgggtggca gggacggggc tgaatcactt tatctgttct
      241 gccgatgcgg taccgggcct gcatcacctg ccggataagg atgagatgcg gggtgttccg
      301 ccagcagatg cgcttcgggc ggtgcagttg ctctggaacg aaatgagcaa ccccgggggg
      361 acgttcccgg tgacgcacga tacttatctg aaactcttcc agttgtccgg tgcagacctg
      421 tctcaccgat gggacaccat cctgt
//
LOCUS       DAZFFI010000537          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFFI010000537 DAZFFI010000000
VERSION     DAZFFI010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.541"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..177
                     /locus_tag="ACHJ12_005590"
     CDS             <1..177
                     /locus_tag="ACHJ12_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3487 family protein"
                     /protein_id="HFO4993174.1"
                     /translation="LLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTFIREA
                     HGWSLRRTRSRGKK"
     gene            174..>442
                     /locus_tag="ACHJ12_005591"
     CDS             174..>442
                     /locus_tag="ACHJ12_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2895 family protein"
                     /protein_id="HFO4993175.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYP"
BASE COUNT           88 a          114 c          139 g          101 t
ORIGIN      
        1 ctgctggtct ggttcggtgg cgtctggatc agccggctga agcggggaaa acctgacaac
       61 tggctctggc agcatctggc gaagaaaagc agtgcatggg gtggtggccg gaaaacattt
      121 atccgggagg cgcatggctg gtccctgcgc cgtacccgta gcagggggaa aaaatgagtc
      181 gttttcgtca tgctctgcgg gatcgcgacc agcatatcct gacactgcgt attttcagcg
      241 gtgtgctggc cggaattctg atgctggtct gtatcgggtg gatgacggca ccggacagac
      301 tgacaattca caatccgccg gatctgcgtt ccggcagtac ccgtccctgg tgggaagtcc
      361 cgccgtcaac ggtttattcc tttgcctttt atatctttca gcaactgaat gcctggccga
      421 agaacggaga ggtggactac cc
//
LOCUS       DAZFFI010000538          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFFI010000538 DAZFFI010000000
VERSION     DAZFFI010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.542"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(179..>442)
                     /locus_tag="ACHJ12_005592"
     CDS             complement(179..>442)
                     /locus_tag="ACHJ12_005592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993176.1"
                     /translation="LPRHPRHRSRDTIEHQGAFPGKRMLMTGNSLIRDRRRATKFLRV
                     SGINWPWGWFGSSSKRQKRAQNVSDSLNPPKHMKPIARCKKVR"
BASE COUNT           95 a           97 c          127 g          123 t
ORIGIN      
        1 gtgttttagc gtgtctccgg cgtgatgtcg agttgtgcgg taatgagtga gcgaaaaaaa
       61 tcccctggat tacaggggga acccatgatc agactttgaa gtttatttgt tatttgatgg
      121 aatgagcacg aatcccggag atgacgttca ggtggcagga aacgcgctca gaagctttct
      181 aacgcacttt tttgcatctg gcaatgggtt tcatatgttt tggtgggttc aggctgtcag
      241 agacgttttg agcgcgtttc tggcgcttgc tgctactgcc aaaccatccc catggccagt
      301 tgatgccaga aacgcgcagg aattttgtag cgcgtctccg gtccctgatc aatgaattcc
      361 ctgtcattaa catgcgttta cccggaaacg cgccctgatg ttctatcgta tcgcgggaac
      421 ggtgtcgagg gtgtcggggc aa
//
LOCUS       DAZFFI010000539          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFFI010000539 DAZFFI010000000
VERSION     DAZFFI010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.543"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..305)
                     /gene="subB"
                     /locus_tag="ACHJ12_005593"
     CDS             complement(<1..305)
                     /gene="subB"
                     /locus_tag="ACHJ12_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFO4993177.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSSVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYY"
     gene            complement(322..>440)
                     /locus_tag="ACHJ12_005594"
     CDS             complement(322..>440)
                     /locus_tag="ACHJ12_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO4993178.1"
                     /translation="SLADKVTEGRVLNAEKAISMFCKKNYIPVRQGRMSEEL"
BASE COUNT          136 a          109 c           72 g          123 t
ORIGIN      
        1 tcataataaa tcctgaccaa ctgacctgtt gtgtaaaaat atagtgcctg attatataat
       61 gtggaaaacg aaggccccca gacagatgaa ttcttcatcg agcaggctct tatggaagaa
      121 ccaccaggtt gtttcccttc aatacaaaaa taagggttat tatctatttg ccctgtatga
      181 aactgatcaa taacaacgct tgaaaacatg ccatcccggg catcgccagt ccactcagcc
      241 atggcaggat taagggataa acatcccata acacctgcac atacaaaaaa cctcttaatc
      301 gtcatattca cctccggaat tttacagttc ttcactcatc cttccctgac ggacaggaat
      361 ataatttttc ttgcagaaca tgctaattgc tttttctgca ttaagaactc tcccttccgt
      421 caccttatct gcaagtgagg
//
LOCUS       DAZFFI010000540          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFFI010000540 DAZFFI010000000
VERSION     DAZFFI010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.544"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..75)
                     /locus_tag="ACHJ12_005595"
     CDS             complement(<1..75)
                     /locus_tag="ACHJ12_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993179.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKK"
     gene            93..284
                     /gene="yfgG"
                     /locus_tag="ACHJ12_005596"
     CDS             93..284
                     /gene="yfgG"
                     /locus_tag="ACHJ12_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4993180.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFVFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          105 a          110 c           84 g          135 t
ORIGIN      
        1 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
       61 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
      121 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt gtcttcttct
      181 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
      241 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac
      301 tttcacagaa cctaacggca cttcgctgtc ggatgctttt gctctttgga attatcaaag
      361 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt
      421 tggctgcgaa ataa
//
LOCUS       DAZFFI010000541          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFFI010000541 DAZFFI010000000
VERSION     DAZFFI010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.545"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..366
                     /locus_tag="ACHJ12_005597"
     CDS             <1..366
                     /locus_tag="ACHJ12_005597"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4993181.1"
                     /translation="TPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGINANLL
                     FKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLR
                     FNGNVSEKLLTLLIQELKR"
BASE COUNT          111 a          123 c          103 g           96 t
ORIGIN      
        1 actcccggca ggcgaaaagg ctgccctaat tatcctcccg aatttaaaca gcagctcgtt
       61 gctgcctcct gtgaacccgg gatatccatc tcaaaacttg ctcttgaaaa tggcattaac
      121 gccaatctgt tgttcaaatg gcgacaacaa tggcgcgagg gaaagctgct attaccttct
      181 tcagagagcc cccagctact tcctgtgact ctcgatgcag ctgccgaaca gccagaatcg
      241 ctcgcagagg acccggaaac cctcagtatc agctgtgagg taacgttccg gcacgggacg
      301 ctccgcttca atggcaatgt cagcgaaaag ctcctgactc tgctgataca ggaactgaag
      361 cgatgatccc gttaccttcc gggaccaaaa tttggctggt tgccggtatc accgatatga
      421 gaaatggctt caa
//
LOCUS       DAZFFI010000542          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFFI010000542 DAZFFI010000000
VERSION     DAZFFI010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.546"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..219
                     /locus_tag="ACHJ12_005598"
     CDS             <1..219
                     /locus_tag="ACHJ12_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993182.1"
                     /translation="HMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYITYA
                     SSAPGHLKDKFIEPLVKRVKDVAEGRAR"
BASE COUNT          118 a           58 c          101 g          156 t
ORIGIN      
        1 catatgtggg atgccatgcc cggtagttat tataaaatgg gtagtgactg ggatggtggc
       61 gaatttgatg atcatctgac gcttgaactt gaaaaaaatg gcaaaggaag tcggatatat
      121 ataacgtatg catcttcggc accggggcat ctgaaagata aatttattga gccattagtt
      181 aaacgtgtga aggatgtggc tgaaggccgg gccaggtaac gtgtactata taatatatcc
      241 cctggcatac atactgccag gggatgttgc ttttctgtat gtcattttat gatgatacct
      301 ttgatattct tatagatgtt taggtgaact gttaatgata aatattgtcc gatattatac
      361 tttcttgtaa gtatattttg ggaaggttat ttttattaag gctttaaatg ttttattttg
      421 ttcgcttttt ctt
//
LOCUS       DAZFFI010000543          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFFI010000543 DAZFFI010000000
VERSION     DAZFFI010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.547"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>433
                     /locus_tag="ACHJ12_005599"
     CDS             <1..>433
                     /locus_tag="ACHJ12_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFO4993183.1"
                     /translation="VDTDAVNVAQLRDLQTVTEKANAALDKRITNNTSAITKNLQVVN
                     SNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNALGIRENTQRIRDE
                     GQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDID"
BASE COUNT          160 a           84 c          111 g           78 t
ORIGIN      
        1 gtcgatacgg atgctgttaa cgttgcacag ctcagggatt tgcagactgt gacagaaaag
       61 gcgaatgcag cgcttgataa acgcataaca aacaacacca gtgcgataac taaaaatctg
      121 caggtggtta acagtaataa agaggaaatc agtaacatta ataaaacgac aaaaattctg
      181 gatgaaagag tgacagccaa tacacagaca gcagaagaac ataagctgct tatttcggag
      241 aacagcaaac aaatcagtac caacgctctt ggtatcagag agaatacaca acggatccgg
      301 gatgagggac aggctcgaat ccggggagac agggaaacac tgcgtgcagc gaccggttat
      361 accgacatgc gggtaaacgg gctggagcgt aatacgaata aacgttttgc gcagcttaaa
      421 gaggatatag aca
//
LOCUS       DAZFFI010000544          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFFI010000544 DAZFFI010000000
VERSION     DAZFFI010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.548"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(88..294)
                     /locus_tag="ACHJ12_005600"
     CDS             complement(88..294)
                     /locus_tag="ACHJ12_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4993184.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          116 a          105 c           84 g          127 t
ORIGIN      
        1 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
       61 gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct tttcggcctg
      121 atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa
      181 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      241 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      301 ctcccccttt tgtatctacc cggtgaatgg caccggaaaa atgaatttgt ttatctgatg
      361 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      421 gatggtttat tt
//
LOCUS       DAZFFI010000545          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFFI010000545 DAZFFI010000000
VERSION     DAZFFI010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.549"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>427
                     /locus_tag="ACHJ12_005601"
     CDS             <1..>427
                     /locus_tag="ACHJ12_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO4993185.1"
                     /translation="AIHHQIIQNQLDTETITISYEFILCQHLIANTEFSYLALPENYN
                     RLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQI
                     LNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          162 a           77 c           74 g          114 t
ORIGIN      
        1 ggctattcat catcagatca ttcaaaatca actggataca gaaacaataa ctattagtta
       61 tgaatttatc ctctgtcaac atcttatcgc aaataccgag ttcagctatt tggcactccc
      121 tgaaaattat aaccgtttgt ttttaccaaa ttcaaaaaac caaacaaata atcgtttcaa
      181 aacgttaaac agcaaagcta ttggcaggct acttgctgct ggtggcgtat acaatgggaa
      241 cattgaagga ttcagagata ctgcggaaaa actgggtgga gatgcaataa aaggctatga
      301 tcaaatacta aatgaaaaaa cagcgggcat agcgatagca acagcatcta ttcttttaac
      361 aaagcgttct aatgttgata catatacaga aataaatagt tacttaggca aacttagagg
      421 tcaacaa
//
LOCUS       DAZFFI010000546          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFFI010000546 DAZFFI010000000
VERSION     DAZFFI010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.550"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>420
                     /locus_tag="ACHJ12_005602"
     CDS             <1..>420
                     /locus_tag="ACHJ12_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFO4993186.1"
                     /translation="VAGKGYRYATRLEAWQALQVFLKHYPAKRIDVGIAQVNLGWNGH
                     RFPSTWAAFDPYTSLNTAAVILQECRRRHPVSWLQVAGCYHHPAGGAPAARYTAVVRR
                     HLARLHRDNFLNPSDVHVAATAMLTPSPRYIWTEPQEE"
BASE COUNT           85 a          118 c          126 g           91 t
ORIGIN      
        1 gtggcaggaa aagggtaccg ttatgccaca cgtctggagg cctggcaggc attgcaggtg
       61 tttctgaaac attatccggc aaaacgtatt gatgtcggga tagcacaggt caatctgggc
      121 tggaatggac atcgtttccc gtccacatgg gcggcatttg atccttacac cagtctgaat
      181 acggcagcgg tcattctgca ggaatgccgg cgtcgccatc cggtcagctg gctgcaggtg
      241 gccgggtgtt atcaccaccc ggcaggtggt gcgccggccg cacgttatac cgccgtggtc
      301 agacggcatc ttgcccggct ccaccgggat aatttcctga atccgtctga tgtgcatgtg
      361 gcagcgaccg ccatgctgac accatccccc cgttatatct ggactgaacc acaggaggag
//
LOCUS       DAZFFI010000547          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFFI010000547 DAZFFI010000000
VERSION     DAZFFI010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.551"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            31..>419
                     /locus_tag="ACHJ12_005603"
     CDS             31..>419
                     /locus_tag="ACHJ12_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO4993187.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQL"
BASE COUNT           85 a           96 c          143 g           95 t
ORIGIN      
        1 tacccgacga cgatgagggg gatgagaatg atgagtgata tgtcgcatca ggaacgtctg
       61 atcaggcggg tcaggaaact gctggagctg agccgtaata acagcaatgc ccatgaggcc
      121 gggctggcgc ttgcaagggc gcaacagctg atggcgaaac acggtatcac ggagcaggat
      181 gccggacttt cttcggtgca gacctcttca tcacaggggg caccttctga tgctgaaaaa
      241 gttccggcgt ggatgtttcg tctggtcatg gtggtgacac agacatttgg ctgccgggcg
      301 tattacgact ggcgggcggt gcccggaggc cggtatcgcc ggtgtgtgac gttttacggt
      361 ttcagtgaac gtcctcaggt ggcggcttat gcgtttgatg ttctgtgccg tcagctaag
//
LOCUS       DAZFFI010000548          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFFI010000548 DAZFFI010000000
VERSION     DAZFFI010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.552"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>415
                     /locus_tag="ACHJ12_005604"
     CDS             <1..>415
                     /locus_tag="ACHJ12_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFO4993188.1"
                     /translation="KNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSW
                     TGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSH
                     KGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP"
BASE COUNT          118 a           77 c          113 g          107 t
ORIGIN      
        1 aaaaaccagt ggaagaacct gggaacgact ccatttccgg gaactgctgg ttcatcggtg
       61 atttttgcag aacagcaaat ttatatacta ggaggggaga gaaaaccggg gcttcgttct
      121 gttagatcct ggacggggga gctttcgcat gatcgaatta aatggtctga acttccacca
      181 gtagcttcac ccgagggggt gtcaggagca tatgcctctg ttattgatgg aaatatcttt
      241 ctggccggtg gtgcatattt cccgggggcg gcagaaaagt atagcaatgg tgaatactgg
      301 tctcataaag gactggataa agcctattca aaagagatat atcagctaat aaaaaatgac
      361 tggaaaaaag ttggttcttt gccggaagga cttgcttatg gtgtaagcct gccct
//
LOCUS       DAZFFI010000549          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFFI010000549 DAZFFI010000000
VERSION     DAZFFI010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.553"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            9..197
                     /locus_tag="ACHJ12_005605"
     CDS             9..197
                     /locus_tag="ACHJ12_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4993189.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
BASE COUNT          137 a           78 c           95 g          104 t
ORIGIN      
        1 gggcagcagt gacttctgca tttgcactgg taatgactgt ttttcttata acgggtgagc
       61 cacagaatgt gattaccgga atttatgcca gtaaagaatc atgcctccgg gcaagagacg
      121 agcaaaaaat ttctggcgaa tgcctcccgt taaaaaaagt atcgctgtac ctgaataacg
      181 aaataccggc tggataatcc accagccata ttaacgccat acccgttgat taagcatgcc
      241 agcaatggca ggggttcgta caaccttaaa atagttatga ggtttatcaa tgagcactga
      301 taaagaagaa tttgcgctat attgcgaagc aaaaaatgac aaggtcagaa aacgctttgg
      361 gattaaaggg ggtttttact ggagtacaga aaagaaatta tctgtagcaa tctc
//
LOCUS       DAZFFI010000550          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFFI010000550 DAZFFI010000000
VERSION     DAZFFI010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.554"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..>410
                     /locus_tag="ACHJ12_005606"
     CDS             77..>410
                     /locus_tag="ACHJ12_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993190.1"
                     /translation="MNITKSAPLPERPCEQLTRCALCVCDPKHKHGNDSRYQAGGQCN
                     QSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSVI
                     VLSCIAMRR"
BASE COUNT           98 a           97 c          105 g          110 t
ORIGIN      
        1 gacgtaatcg catggatgaa agaacgtgag caggaagcac gcggaacggc tgcgtaatgg
       61 tgtgaggctt gacacgatga acataacaaa aagcgccccg ttgccggaac gcccttgtga
      121 acaattaacc cgctgcgcct tatgtgtatg tgatcccaaa cataagcacg ggaatgatag
      181 ccgctatcag gctggtgggc aatgcaatca gtctggttca gttcgttgcc atacctgcaa
      241 tgagcgcttt tccctgtact ctttaaggaa ttgctcaagg gcaaaagcac atggcgcgaa
      301 tctttctgat tcatgctcta tctttctgcg ccgtcttttc cgtgccggtg ataatgtttt
      361 ggtcaattct ttatcggtca ttgtgttgtc ctgcatagca atgcgccgta
//
LOCUS       DAZFFI010000551          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFFI010000551 DAZFFI010000000
VERSION     DAZFFI010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.555"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>404)
                     /locus_tag="ACHJ12_005607"
     CDS             complement(<1..>404)
                     /locus_tag="ACHJ12_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO4993191.1"
                     /translation="EVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAVARIAGTAG
                     SAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLITLTFVVFAV
                     HFLTFWWELARWLDSWMLTALYSSDTHTRFNM"
BASE COUNT          100 a          134 c           88 g           82 t
ORIGIN      
        1 atcatattga accgggtatg tgtatccgaa ctgtacagcg ccgtcagcat ccagctgtca
       61 agccaccgtg caagctccca ccagaacgtc agaaagtgta ccgcaaacac cacaaacgtc
      121 agtgtgatta acgtcgacag ttcatatgcc gcaaacgcca gaatcagcgg tatcatgatg
      181 taaatcgcca tcagtattat cgactgtacc atcggcagtg cctgccgcat tgcatccagt
      241 gccggaaacg cacccagtgc tgctaccgca ctgcccgccg ttcccgcaat ccgggccacc
      301 gcattcatgg tcgtcatatc tgcattgcca ccatagccac gatacacctc tcccccctga
      361 gagagcgtca ggtttgccgg actcaccaga cgcttgatca cctc
//
LOCUS       DAZFFI010000552          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFFI010000552 DAZFFI010000000
VERSION     DAZFFI010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.556"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>402
                     /locus_tag="ACHJ12_005608"
     CDS             <1..>402
                     /locus_tag="ACHJ12_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HFO4993192.1"
                     /translation="NQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDRD
                     ILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEKL
                     RTPPEPGTITGEQARGEKTVNALMDTFPEGT"
BASE COUNT           82 a          104 c          146 g           70 t
ORIGIN      
        1 tgaaccaggt ctgttcgcgg gtcgtggggg cgctgggtgg cgcaggcgtc cgctgtacac
       61 ggatgaacgg gcagcaggtg catggctggc tgctgaggct gttcaatccc cggccggaat
      121 gggtggacag ggatattctg taccgtatgg catcccgtgc agaaccgcag gagacaccgg
      181 agggaatgat gccggtgatg accgattttg cagaaagcct ctggttcacc ccaccggtat
      241 ctgatccgga aaacggcgtg tggtggctgg acgggcttcc gcatgccgct gttgtggtgg
      301 agaaactgcg cacaccaccg gagccgggaa ccatcacagg ggaacaggcc cggggcgaaa
      361 agacagtgaa tgccctgatg gacacctttc cggagggaac gg
//
LOCUS       DAZFFI010000553          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFFI010000553 DAZFFI010000000
VERSION     DAZFFI010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.557"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /locus_tag="ACHJ12_005609"
     CDS             <1..200
                     /locus_tag="ACHJ12_005609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993193.1"
                     /translation="AEQKTCCIVKRNPRRHVHTPQAGYLTPDHCRVTRSIALRSVGRG
                     GFMDTIYKLKFNWSTCLLLNL"
     gene            246..>401
                     /locus_tag="ACHJ12_005610"
     CDS             246..>401
                     /locus_tag="ACHJ12_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO4993194.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILK"
BASE COUNT          121 a           83 c           96 g          101 t
ORIGIN      
        1 tagcggaaca aaaaacctgc tgcatagtta aacgtaatcc ccgccgtcat gttcatacgc
       61 ctcaggctgg ctacttaacc cctgaccact gccgggtaac tcgaagtatt gccctgcgtt
      121 ctgtggggcg gggtgggttt atggatacaa tttacaaatt aaaatttaac tggtcaacat
      181 gtttattatt aaatttgtaa ttgcgagctg ttgcattagt cccaaaatgg gactactata
      241 cagttatgaa gattatctca gttaaaacac tcagggattt ttgggcggag aatcctgatg
      301 cagaacaacc gctaagggca tgggtggatg aggcggcaaa agctgactgg aaaagcccag
      361 cagacattaa ggcacaatac cgaacggcca gtatattgaa a
//
LOCUS       DAZFFI010000554          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFFI010000554 DAZFFI010000000
VERSION     DAZFFI010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.558"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          125 a           81 c           68 g          125 t
ORIGIN      
        1 ccagattaaa tacttgagta taaaatctct gctgatgttt aattgatttg aatgttcgta
       61 agctatatca cttactcaat tcattttacc cagagtcata aaatagagtg tctgttgatc
      121 tcattttcat attcctttca gactgaagtg cattttcatc acccgatgag aaagaactga
      181 agtgatgttt gccatagatt acgagatgcc cagtctcatt gcctatcgtg gtgcaccttt
      241 atacctgtta taccagatca aaaatcacgc aatccataca acgaaaccag atttgcaatt
      301 cgtgtcacaa aatatgtcga tctttttcta agaagaagat gccatgtgaa gccagacgaa
      361 cacttgcgga ggccttcaca aaccaaagat tgttgttta
//
LOCUS       DAZFFI010000555          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFFI010000555 DAZFFI010000000
VERSION     DAZFFI010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.559"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(106..>399)
                     /locus_tag="ACHJ12_005611"
     CDS             complement(106..>399)
                     /locus_tag="ACHJ12_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4993195.1"
                     /translation="NYMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKG
                     RLEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
BASE COUNT          102 a          109 c           81 g          107 t
ORIGIN      
        1 ccgctggcga aaaaaaacgc ctgcagacat gcaggcgctt tgttgacagg aaggaaagca
       61 acataacaaa tatgtcacaa tcctgcggtt aacataaatt accgattacc ggagagcatt
      121 acgcaaatca tcatccgaga actgcagtat ttgtttgatt ttctctgacg atatacccat
      181 ctgctgcatg cgacgggccg atgcgtaatt ctcctgaaga gccttttctc tctcctcctg
      241 gcggcccttc tcgatacctt ccaggcggcc cttctcgata ccagccagtt ccagtcgttc
      301 agcgatagtc attagatcat cctcatgtct tggcagacgt tctgccatcg cccgtataaa
      361 ttcaggtgaa gctgaatcac attctttcac catatagtt
//
LOCUS       DAZFFI010000556          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFFI010000556 DAZFFI010000000
VERSION     DAZFFI010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.560"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..93
                     /locus_tag="ACHJ12_005612"
     CDS             <1..93
                     /locus_tag="ACHJ12_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO4993196.1"
                     /translation="SQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            90..>394
                     /locus_tag="ACHJ12_005613"
     CDS             90..>394
                     /locus_tag="ACHJ12_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4993197.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPA"
BASE COUNT           92 a           93 c          120 g           89 t
ORIGIN      
        1 tcacaactga atgaagtaca gattgtggtt gatgagatcc gcgacacact ggaaaaggca
       61 gtcattgact gttgtgatga ggccgatatg tgaatcaggt gaacgagagc catagccgcg
      121 catccgatat ctggcgcgaa gtggcctcgc tgtttcgccc acctggccgg ttaccagtag
      181 cggaagccat caggcgttat atgcgggtac cacggggagc caatacttcc ggtccgtggg
      241 agtcatcgct gacgccctat atgattgacc ccattaatac attatcagcc cgtgaatatg
      301 acgcggtggt gtttgtggga cctgcgcgaa ccgggaaaac cgaagggctg attgatggct
      361 ggattgtgta cggcatcatc tgtgatccgg cgga
//
LOCUS       DAZFFI010000557          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFFI010000557 DAZFFI010000000
VERSION     DAZFFI010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.561"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>393)
                     /locus_tag="ACHJ12_005614"
     rRNA            complement(<1..>393)
                     /locus_tag="ACHJ12_005614"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a          116 c           91 g           94 t
ORIGIN      
        1 ctccgttact ctttaggagg agaccgcccc agtcaaacta cccaccagac actgtccgca
       61 acccggatta cgggtccacg ttagaacatc aaacattaaa gggtggtatt tcaaggtcgg
      121 ctccatgcag actggcgtcc acacttcaaa gcctcccacc tatcctacac atcaaggctc
      181 aatgttcagt gtcaagctat agtaaaggtt cacggggtct ttccgtcttg ccgcgggtac
      241 actgcatctt cacagcgagt tcaatttcac tgagtctcgg gtggagacag cctggccatc
      301 attacgccat tcgtgcaggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      361 ttatagttac ggccgccgtt taccggggct tcg
//
LOCUS       DAZFFI010000558          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFFI010000558 DAZFFI010000000
VERSION     DAZFFI010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.562"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>393
                     /locus_tag="ACHJ12_005615"
     CDS             <1..>393
                     /locus_tag="ACHJ12_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4993198.1"
                     /translation="ARQRVTEDRMQPGSETEPGVETQSSVLKMLSSDSELSEKQGQKI
                     LSSDSEPSEKSEGYHRVRKPNHYVRNITHSVIKNTYVLPEALTGQIREEEAIMLTSQL
                     QALPEELAQGVLESLSGMLGSHSLQNPVG"
BASE COUNT          117 a           85 c          119 g           72 t
ORIGIN      
        1 gcccgtcagc gggtgactga ggacagaatg cagccgggtt cggaaaccga accgggggta
       61 gaaacacaga gttcggtatt gaaaatgctg agttcggatt ccgaactcag tgaaaaacag
      121 gggcaaaaaa tactgagttc ggattccgaa ccatcagaaa aatcagaagg ttatcacaga
      181 gttcggaaac cgaaccatta cgtacgtaat atcacacaca gtgtgattaa aaacacgtac
      241 gtattgcctg aagcgctgac agggcaaatc agggaggagg aggccattat gctgaccagt
      301 cagttgcagg cgctgccgga agaactggcg cagggggttc tggaaagtct gtcagggatg
      361 ctgggcagcc actcgctgca gaatcctgtg ggc
//
LOCUS       DAZFFI010000559          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFFI010000559 DAZFFI010000000
VERSION     DAZFFI010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.563"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..385
                     /locus_tag="ACHJ12_005616"
                     /pseudo
     CDS             1..385
                     /locus_tag="ACHJ12_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           96 a          105 c          107 g           77 t
ORIGIN      
        1 gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga ttgcacgtct
       61 caaacgccag ctggcagaac gggatgaaga gctggctatc ctccaaaagg ccgcgacata
      121 cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc
      181 atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag
      241 cggcggacaa ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc
      301 gcggctttta cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt
      361 gctcagggtt acccctttaa cgtaa
//
LOCUS       DAZFFI010000560          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFFI010000560 DAZFFI010000000
VERSION     DAZFFI010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.564"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(22..366)
                     /locus_tag="ACHJ12_005617"
     CDS             complement(22..366)
                     /locus_tag="ACHJ12_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4993199.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
BASE COUNT           93 a          126 c           94 g           67 t
ORIGIN      
        1 ttttgtagaa aaaagaaaaa gtcacaccgt gacctgaaac tccagggttg cacggtaaca
       61 gcggttttcc ggaatatagt cctgcatttc actgacggat cccggggcca gcagcattat
      121 ggcttcacgg gcgtcctgac gtatctgacg cgcctgcgtc acagtcccgg cataaacgtc
      181 tatctgcacc gccactgagg actccgcctg cccgcccatc acgtccgcag acaccgatga
      241 aatcaggctg aaaaccaccc acggaagcgc caccgacggc ctgccatcca gcagggggac
      301 cacatacggg tacacctgcc cgccggcaag atgcgccaga tgaggataca aatccgcctc
      361 cgtcatcgtc tcagtacctc
//
LOCUS       DAZFFI010000561          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFFI010000561 DAZFFI010000000
VERSION     DAZFFI010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.565"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            121..>376
                     /locus_tag="ACHJ12_005618"
     CDS             121..>376
                     /locus_tag="ACHJ12_005618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993200.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSP"
BASE COUNT           96 a          100 c           86 g           94 t
ORIGIN      
        1 ttataaacta cgttagaggt tttgttataa ggtaatcggt ttctttacag accgctggcg
       61 tacatcgcac agaggctgct aacagggata ttcctgccta gatgaacgca aggcaagatc
      121 atggaaatag tgatatacat cactatttcc atcctgattt tagatatcgc attaataaaa
      181 cagcggatgt tagccaatat cgaaaccagt aacccgtatc acgcccagta catcctgcgc
      241 cgccgcgatg attacctggt gctgttcgac cgtgatgccc tgagctgccg cttcttttat
      301 gacgcctttc cgggaatgcg tctgcgccac caagtgaccg gcgataccga cgatgaccgc
      361 ctggcacaca gccccg
//
LOCUS       DAZFFI010000562          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFFI010000562 DAZFFI010000000
VERSION     DAZFFI010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.566"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHJ12_005619"
     CDS             <1..250
                     /locus_tag="ACHJ12_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993201.1"
                     /translation="QIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESA
                     VMQFARVARKSGKQLIVAVREQDMKKMEAEFGRIIKIH"
BASE COUNT          117 a           66 c           97 g           94 t
ORIGIN      
        1 ccagatttac agttgttctg acagaacatt aaatgattat gcggttattt ttatcaggga
       61 tgcactgaac gccagtgagc atattattat ttttaattgt acaggatggc ctgatttgaa
      121 taatgaatct gccgtcatgc agtttgccag agtggcacga aaatcaggaa aacaactgat
      181 agtcgcagta agagaacagg atatgaaaaa aatggaagca gagtttggaa ggatcataaa
      241 aatccactga cgccttcgcg ttgctcagtt gtccaacccc ggggacggga aaaagcaagt
      301 tttccccgcc cccggcgttt ctgtagtgaa aagcgggaaa tggagggaat ggtcagtaac
      361 agggaatgtg gtat
//
LOCUS       DAZFFI010000563          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFFI010000563 DAZFFI010000000
VERSION     DAZFFI010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.567"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(124..>372)
                     /locus_tag="ACHJ12_005620"
     CDS             complement(124..>372)
                     /locus_tag="ACHJ12_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993202.1"
                     /translation="SLYLQKERPDFCKGTFGIVPLMTEYTDSGGNEFTGYRICVYFDA
                     YGNGTEDTWTSLNIMFDGLMKATKRMSNETITGEMRPL"
BASE COUNT          102 a           75 c           93 g          102 t
ORIGIN      
        1 gatgagcagt tcgcggcgtt ttacgattat tgccggaaag tgatgagcag aaatctcgca
       61 gagtgtttcc gtattcaaaa aaacagagag cgctctgcaa atagtgagat tctggataat
      121 tttttaaagg ggacgcatct cgccagtgat tgtttcattg ctcattcttt tggtggcttt
      181 catgaggcca tcaaacatga tgttgagaga agtccaggta tcctctgttc cattcccgta
      241 agcatcgaaa tagacacaaa tacgataacc ggtgaactcg ttaccgccgg agtcggtata
      301 ttccgtcatg aggggaacga tgccaaaggt tcctttacaa aaatcgggcc gttctttctg
      361 aaggtagagc ga
//
LOCUS       DAZFFI010000564          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFFI010000564 DAZFFI010000000
VERSION     DAZFFI010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.568"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..>368)
                     /locus_tag="ACHJ12_005621"
     CDS             complement(149..>368)
                     /locus_tag="ACHJ12_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO4993203.1"
                     /translation="GKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHV
                     RQRTMKKELEKLTSADEILAYRVGWGDP"
BASE COUNT           84 a          105 c           67 g          112 t
ORIGIN      
        1 gaaaaatctc aatagcagta aaaagtaact gttcagttaa agtgtaatat ttacctaagt
       61 aacttttcct gtgtattacc gtgaaggagc ataattggtt attgggcgtt ctccttccat
      121 aaaaatacgc agtgtcctcc tttccagatt acggatcacc ccagccaacc ctgtatgcca
      181 gtatctcatc cgcactggtc agcttttcca gctctttctt catggtgcgc tgccggacgt
      241 ggatttccat ccctttggtg aacatcgcct gctctgccgc ttcactcagc gctataagct
      301 cttctgctgt cacttccaca tcattgtttt ccgcatccgt ccagaaaaac gcctccggca
      361 gtttcccc
//
LOCUS       DAZFFI010000565          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFFI010000565 DAZFFI010000000
VERSION     DAZFFI010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.569"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..>367
                     /locus_tag="ACHJ12_005622"
     CDS             43..>367
                     /locus_tag="ACHJ12_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993204.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVM"
BASE COUNT          107 a           69 c          105 g           86 t
ORIGIN      
        1 agaatgagca ctactgtcat tcacatcaaa ggggaatgag atatggctat tgcagagaga
       61 acgggtatct cctcgcgcgg cgatacttac gaaatctggt tgggagcaaa ctgttatcac
      121 taccgctctg atggttatgc agtgcttctg aaagaaggca ggaatcgtgc ttatccatgt
      181 cgggtgacga gtgaaacgat taaatctgcg ctggcagcaa ttgttgaatt gcaacagaaa
      241 ttaactgcgg cagagaagaa aaaggagtgt gagaaccacg gggttctttt gccagctgag
      301 atgttaattc gctgtctgga agatcaaaca gggggacggt ggatccgtgc acatgaacca
      361 gtaatgc
//
LOCUS       DAZFFI010000566          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFFI010000566 DAZFFI010000000
VERSION     DAZFFI010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.570"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>366
                     /locus_tag="ACHJ12_005623"
     CDS             <1..>366
                     /locus_tag="ACHJ12_005623"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4993205.1"
                     /translation="KSLDELKSIPDVIIKHLGQNRGIATAQNVGLRISQSLCSKYALL
                     LDQDSEIETDLVSKLLDGYITLEQKGIKLAAVGPRPFDLFENKKMKASIQRDIQIFEG
                     FSLTKQIIASGKLINLDVL"
BASE COUNT          132 a           59 c           67 g          108 t
ORIGIN      
        1 aaaaagttta gacgaattaa aaagtattcc agatgttata attaagcacc ttggtcaaaa
       61 ccgtggtatt gcaacggcac aaaatgtcgg gctaagaatt tctcaatctt tatgctcaaa
      121 gtatgcgcta ttactggatc aagattcaga aattgaaaca gatttagtgt caaaacttct
      181 cgacggttac attacattag aacaaaaagg catcaaactt gccgctgttg gtccccgacc
      241 gtttgattta tttgaaaaca aaaaaatgaa agcaagcatt caaagagata ttcaaatatt
      301 tgagggtttt tctttgacga aacagataat cgcttcaggt aagctaatta atttggacgt
      361 tttaga
//
LOCUS       DAZFFI010000567          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFFI010000567 DAZFFI010000000
VERSION     DAZFFI010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.571"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>366)
                     /locus_tag="ACHJ12_005624"
     CDS             complement(<1..>366)
                     /locus_tag="ACHJ12_005624"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFO4993206.1"
                     /translation="LEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSA
                     VVQDVVQKREKTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTD
                     CGYLPASMHEESSEVLEAV"
BASE COUNT           91 a          118 c           82 g           75 t
ORIGIN      
        1 cgacagcctc aagcacttca gaactctcct catgcatcga ggcaggcaga taaccacagt
       61 ctgtcctggg ataagttgtt gctttgtgag tctcataaag ggactgtgcg atatccagaa
      121 cggtctgagc ccccatgcca aacttccggg aacaggcttc ctgcaacgtt cccagcgaaa
      181 aacacaaagg tggcggtgtt ttctccctct tctgtacaac atcctgtaca acagcagagg
      241 actgctgacg gcacagaccg agaacagcac cggccgcctg cacattcaca caacgtccct
      301 catcatcgca gtactgctca gctgccaccc agttactgcg gaaacggagc ccctcctttt
      361 ccagcc
//
LOCUS       DAZFFI010000568          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFFI010000568 DAZFFI010000000
VERSION     DAZFFI010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.572"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..111
                     /locus_tag="ACHJ12_005625"
     CDS             <1..111
                     /locus_tag="ACHJ12_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4993207.1"
                     /translation="GSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            108..>365
                     /locus_tag="ACHJ12_005626"
     CDS             108..>365
                     /locus_tag="ACHJ12_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4993208.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREEL"
BASE COUNT           86 a           87 c          126 g           66 t
ORIGIN      
        1 ggctcgctgt ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca
       61 ggctggaccc tggctgcaaa acgtgggcgg gatgctggag gcggaaaatg acggcattac
      121 tgacactgga agagatcaag gcacatctgc gtgtcgacca tgacgcggat gatgacatgc
      181 tgatggacaa ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggcagccggg
      241 ataaggtgat tcgtgaggac ggtgaactga tcccgggcga ggcattaacc cggatgaagg
      301 gggctgccat gcgactgacc gggatgctgt accggaatcc ggatcttgcg gagcgggaag
      361 aactg
//
LOCUS       DAZFFI010000569          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFFI010000569 DAZFFI010000000
VERSION     DAZFFI010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.573"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..111
                     /locus_tag="ACHJ12_005627"
     CDS             <1..111
                     /locus_tag="ACHJ12_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4993209.1"
                     /translation="GSLWHCNSMTGDKPGEAHSSGWALAAKRGRDAGGGK"
     gene            108..>365
                     /locus_tag="ACHJ12_005628"
     CDS             108..>365
                     /locus_tag="ACHJ12_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4993210.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMEKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREEL"
BASE COUNT           86 a           86 c          128 g           65 t
ORIGIN      
        1 ggctcgctgt ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca
       61 ggctgggccc tggctgcaaa acgtgggcgg gatgcaggag gcggaaaatg acggcattac
      121 tgacgctgga agagatcaag gcacatctgc gtgtcgacca tgacgcggat gatgacatgc
      181 tgatggagaa ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggcagccggg
      241 ataaagtgat tcgtgaggac ggtgaactga tcccgggcga ggcattaacc cggatgaagg
      301 gggctgccat gcgactgacc gggatgctgt accggaatcc ggatcttgcg gagcgggaag
      361 aactg
//
LOCUS       DAZFFI010000570          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFFI010000570 DAZFFI010000000
VERSION     DAZFFI010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.574"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          104 a           93 c           71 g           92 t
ORIGIN      
        1 acgttgataa tgctgtcaac gctaacttcc gttactcgtt ctgatactcc gtattcaaaa
       61 gggcctgagg gccctttatg ctcaggacct gccgcccagg tatacatttc cggagtatca
      121 gtcgatttgt ttacttaacc gccatgacca aagccggcag taatgtcggc tttttacgtc
      181 catatattcc actcatccgg aactgtgaca cagaacctga atacaggatt gatcaccggt
      241 gcccataaat ggcacatttt cagtaagatt ctgaatgtta cattccccct tcatgtgtga
      301 cacagctgtc acagagaacg aaacggaaag cacatgaaaa acataaaaac tcacaccgga
//
LOCUS       DAZFFI010000571          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFFI010000571 DAZFFI010000000
VERSION     DAZFFI010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.575"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..343
                     /locus_tag="ACHJ12_005629"
     CDS             <1..343
                     /locus_tag="ACHJ12_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4993211.1"
                     /translation="TLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRA
                     VSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
BASE COUNT           82 a           99 c          105 g           73 t
ORIGIN      
        1 gaccctgtcg cgacactcag agttggcgaa ggcgttcgcg tacgcactta accagtggcc
       61 ggcactgacg tactatgcga acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa
      121 tgccctgcgg gcggtcagtc tgggtcgtaa aaacttcctg ttcttcggct ctgaccatgg
      181 tggtgagcgg ggagcgctac tgtacagcct gatcgggacg tgcaaactga atgacgtgga
      241 tccagaaagc taccttcgcc atgtgcttgg cgtcatagca gactggccgg tcaaccgggt
      301 cagcgaactg cttccgtggc gcatagcact gccagctgaa taacacatcc ccgtcaata
//
LOCUS       DAZFFI010000572          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFFI010000572 DAZFFI010000000
VERSION     DAZFFI010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.576"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>359
                     /gene="espX1"
                     /locus_tag="ACHJ12_005630"
     CDS             <1..>359
                     /gene="espX1"
                     /locus_tag="ACHJ12_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4993212.1"
                     /translation="DLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGR
                     WTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNK
                     FMHDYDKTSSKMRKYLQ"
BASE COUNT          138 a           69 c           56 g           96 t
ORIGIN      
        1 gcgacttatc aaacaccatc ttttcaaatg gagaaataaa caacctcttc attaaaaaaa
       61 gcaacatatt tggaacatcg ttcactaata ccatgataaa aaatttgcgc tgtgaagata
      121 ttatgcctgg ccgatggacc actcaacttg tcaataagca cttaggttac agatacacag
      181 gagtattcaa aaccctggca agtattgatg acaaaccgtc ccgtttcgaa attttgatac
      241 cattggtaca aactttagtt cgcgacaatg taaagctaaa taatgatgtg tataaagaac
      301 ttaataagtt tatgcacgat tacgacaaaa catcatcaaa aatgagaaaa taccttcag
//
LOCUS       DAZFFI010000573          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFFI010000573 DAZFFI010000000
VERSION     DAZFFI010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.577"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>359
                     /locus_tag="ACHJ12_005631"
     CDS             <1..>359
                     /locus_tag="ACHJ12_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO4993213.1"
                     /translation="SCVRGQVNSITFVFADGTVRTLPSSDVNGSSNAQAKNNSSTGGI
                     GWISDDDGIPCISGTRKSNASTYLPTIFALSAAGAAGDALSEGQYTSQNNVNGITSTL
                     TGSAGEAALGKALSGGM"
BASE COUNT           89 a           95 c          107 g           68 t
ORIGIN      
        1 tcctgtgtcc gtgggcaggt gaacagcatc acgtttgttt ttgccgacgg gacagtgcgt
       61 acgctgccgt catcagacgt gaacggcagc agtaacgcac aggcaaaaaa taacagcagt
      121 acaggaggca ttggctggat ttcggatgat gacgggatcc cgtgtatcag cggaacccgt
      181 aaatccaatg cctccacgta tctgccgaca atatttgccc tgtctgcagc cggcgctgcg
      241 ggtgatgcac tgagtgaggg gcagtacacc tcacagaaca acgtcaatgg catcacatca
      301 acactgaccg gcagtgccgg agaggcggca cttggaaaag ccctgtccgg cgggatgaa
//
LOCUS       DAZFFI010000574          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFFI010000574 DAZFFI010000000
VERSION     DAZFFI010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.578"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>350)
                     /locus_tag="ACHJ12_005632"
     CDS             complement(<1..>350)
                     /locus_tag="ACHJ12_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4993214.1"
                     /translation="ETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGS
                     FLKIGWPSITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVE
                     SSPGRDITDTKWRCG"
BASE COUNT           82 a           93 c          100 g           75 t
ORIGIN      
        1 ccacagcgcc atttggtgtc agtgatatcc cgcccgggcg aactttcgac cagggtcatc
       61 cccgaggaca taaaggtggt ggtacgcttt gaggccaggg tgaaggcatc cccttcaccg
      121 tcgacgttct caggaaaacg gtcataatcc gtcagcgcca cacgacggta atccgaagag
      181 gaaaatacag tgatcgacgg ccagccaatt ttcaggaatg agccgtcaag aaacattttg
      241 tcgtggacgt tgttgtcatt acgggaaggg ctgaggcgct tactgacctc cggactgtgg
      301 cgaaacgtcc tggaaagacg cgttctggaa tgctcacgcg cctttgtctc
//
LOCUS       DAZFFI010000575          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFFI010000575 DAZFFI010000000
VERSION     DAZFFI010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.579"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          113 a           85 c           65 g           87 t
ORIGIN      
        1 cccttaattg tacgtaccgc tgaaacgctc tcatacgcgt tctgacgcaa aaacgcaaaa
       61 agacaaggaa accccaggct aaaccgaaaa aatgcgtcag aatcgttttc aggtacttgc
      121 ataactattc acaaaagcga tgcgcgatcg atttcgacta ttccgacgga cgaacaggtt
      181 aaaatgataa gtttcctccc tccggatgga aggttcagca cggggatttt tacccccttc
      241 catcaacctg aatatgcatc tgggagtacc cattagtttc tactcaaaca ctatcgtagt
      301 gctatctcaa aatataaaac ataccgatgg cgtaagcatt ttaaatatga
//
LOCUS       DAZFFI010000576          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFFI010000576 DAZFFI010000000
VERSION     DAZFFI010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.580"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>350
                     /locus_tag="ACHJ12_005633"
     CDS             <1..>350
                     /locus_tag="ACHJ12_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4993215.1"
                     /translation="ETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGS
                     FLKIGWPSITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVE
                     SSPGRDITDTKWRCG"
BASE COUNT           76 a           95 c           96 g           83 t
ORIGIN      
        1 gagacgaagg cgcgtgagca ttccagaacg cgtctttcca ggacgtttcg ccacagtccg
       61 gaggtcagta agcgcctcag tccttcccgt aatgacaaca acgtccacga caaaatgttt
      121 cttgacggct cattcctgaa aattggctgg ccgtcgatca ccgtattttc ctcctcggat
      181 taccgtcgtg tggcgctgac ggattatgac cgtttccctg aaaacgtgga cggtgaaggg
      241 gatgccttca cgctggcatc aaagcgtacc accaccttta tgtcctcggg gatgaccctg
      301 gttgagagtt caccggggcg ggatattacc gataccaaat ggcgttgtgg
//
LOCUS       DAZFFI010000577          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFFI010000577 DAZFFI010000000
VERSION     DAZFFI010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.581"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>344
                     /locus_tag="ACHJ12_005634"
     CDS             <1..>344
                     /locus_tag="ACHJ12_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4993216.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZFFI010000578          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFFI010000578 DAZFFI010000000
VERSION     DAZFFI010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.582"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>341
                     /locus_tag="ACHJ12_005635"
     CDS             <1..>341
                     /locus_tag="ACHJ12_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4993217.1"
                     /translation="PDDVRPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANI
                     PQPADGKSITPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVSALPAPQ
                     DGRDATALEILP"
BASE COUNT           76 a           79 c          112 g           74 t
ORIGIN      
        1 ccggatgatg tgcgtccgat gcttgaacag atggtgaaag aggcagtgag ccatattcct
       61 gttccgcgtg atggtcgtga ctacgatccg gaagtactgc agaaggcggt gaatgatgca
      121 gtcgcaaata ttccgcagcc ggcagacggt aaaagtatca ccccggatga tgtgcgtccg
      181 atgcttgagc agatggtgaa ggaggcggta agccatattc ctgttccgcg tgatggtcgt
      241 gactatgatc cggatgttct gcagaaggcg gtgaatgatg cggtgagtgc cctgccggct
      301 ccgcaggacg ggcgtgatgc cactgcactg gaaatactcc c
//
LOCUS       DAZFFI010000579          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFFI010000579 DAZFFI010000000
VERSION     DAZFFI010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.583"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>338)
                     /locus_tag="ACHJ12_005636"
     CDS             complement(<1..>338)
                     /locus_tag="ACHJ12_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993218.1"
                     /translation="LAALEGDENAFFSAEVNMVRRELQRVPGDSVNCMCWASLAALAF
                     DAEERVELQKGNNENIATYDESIATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILF
                     PRDGADIFPD"
BASE COUNT           83 a          102 c           80 g           73 t
ORIGIN      
        1 gtcggggaaa atgtcagcac catcacgggg aaacaaaatc gctttccatt cagggtatga
       61 gcgcggaata cctccggaag cattccggct cagacagcga ctcacagtgc aggcagcacg
      121 gaatgcatat gttgcgatgc tctcatcata tgttgcgatg ttctcattat tgcctttctg
      181 cagttccacc cgctcttcag catcaaatgc cagtgcagca aggcttgccc agcacatgca
      241 gttcactgaa tcccccggaa cgcgctgcag ctcccgacgc accatgttga cctccgcact
      301 gaaaaaagcg ttctcatcgc cttcaagggc agccagat
//
LOCUS       DAZFFI010000580          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFFI010000580 DAZFFI010000000
VERSION     DAZFFI010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.584"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>335
                     /locus_tag="ACHJ12_005637"
     rRNA            2..>335
                     /locus_tag="ACHJ12_005637"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           72 c          104 g           62 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgcgcg atacagggtg acagc
//
LOCUS       DAZFFI010000581          334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFFI010000581 DAZFFI010000000
VERSION     DAZFFI010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.585"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>334)
                     /locus_tag="ACHJ12_005638"
     CDS             complement(<1..>334)
                     /locus_tag="ACHJ12_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4993219.1"
                     /translation="RITPDRNCGIPEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGL
                     AATQGHHIQENSTSFFRNGGRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAI
                     LSNGAKYNP"
BASE COUNT           62 a          105 c           85 g           82 t
ORIGIN      
        1 ggggttgtat tttgccccgt tgctcagaat ggccgttttc cccgcatttt cgcctgtata
       61 cccgctgtcc cagttgctct tcagtttttt cgcattttct tccgtaatac tgccggggat
      121 ctcaatcacc ccggacggcc tgccgccatt tctgaaaaaa gacgtcgaat tttcctgaat
      181 atgatgcccc tgcgtggccg ccagcccggc ggcatacacc ggcggcaacc ctataagcgg
      241 atgaaaaaaa cagttaaacc ggtcgtggat cacttcccgg gcaggcaccg tcaccgcctc
      301 agggatcccg cagttccggt ccggcgtgat gcgg
//
LOCUS       DAZFFI010000582          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFFI010000582 DAZFFI010000000
VERSION     DAZFFI010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.586"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..120
                     /gene="gpU"
                     /locus_tag="ACHJ12_005639"
     CDS             <1..120
                     /gene="gpU"
                     /locus_tag="ACHJ12_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4993220.1"
                     /translation="PGLSALLLKMNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            128..>333
                     /locus_tag="ACHJ12_005640"
     CDS             128..>333
                     /locus_tag="ACHJ12_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4993221.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVS"
BASE COUNT           83 a           78 c           97 g           75 t
ORIGIN      
        1 cccgggctaa gtgcattact gctgaagatg aatcttcagg ggtatgacta tcgccgggat
       61 gacgaatata tgatgtgggg atcggctgat ctccagtgga atattactta ccagatgtga
      121 ggacgttatg acgctcccta atccccttgc gccggtaaaa ggttccggca ccacgctctg
      181 ggtttatacc ggaaatggcg atgcctgggc aaacccgctg tcagatgatg actggacgcg
      241 cctggcaaaa ataaaggatc tgacccccgg cgagatgacg gcggaatcct acgacgataa
      301 ctatctggat gatgaggatg ctgactgggt atc
//
LOCUS       DAZFFI010000583          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFFI010000583 DAZFFI010000000
VERSION     DAZFFI010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.587"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>327
                     /locus_tag="ACHJ12_005641"
     CDS             14..>327
                     /locus_tag="ACHJ12_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993222.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDT"
BASE COUNT          120 a           54 c           55 g           98 t
ORIGIN      
        1 ttacaggtta tttatgaaag tatttcacaa tatatttaag cacttctctt caaaccatca
       61 agataagcat tcagataaag ttaacagtca tcaacaccat ggcaaggttg ataaaacaca
      121 tcgtgcaaag atagttgaat ttgataaact ggataatgat tcgcaaatag ataacgattt
      181 tggcctgcat atcatctatt tcctgcaaca tggtcactgg aaggtaaatg atcgtagtca
      241 ccagatggag aaagtatggt tttataatag tgaaccttct atagatattc aagaatataa
      301 tagattcgcc gacaatacta ctgatac
//
LOCUS       DAZFFI010000584          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFFI010000584 DAZFFI010000000
VERSION     DAZFFI010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.588"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>325
                     /locus_tag="ACHJ12_005642"
     CDS             <1..>325
                     /locus_tag="ACHJ12_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4993223.1"
                     /translation="VLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQS
                     EDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDD
                     KGRLTG"
BASE COUNT           76 a           93 c          106 g           50 t
ORIGIN      
        1 gtgctgacca acgagaacgg cagccagtcc acgttccggt atgacccggt cgacaggctg
       61 acggagcagc gcggctttga cggccggacg caacgttacc agtatgacct gaccggaaaa
      121 ctcacgcaga gtgaagatga ggggcttatc accctctggc attacgatgc gtcggaccgt
      181 atcacccggc gcacggtgaa cggtgaaccg gcggagcagt ggcagtatga cgaccacggc
      241 tggctgacag aaatcagcca cctgagtgaa ggccaccgtg tggcggtcca ctacggctat
      301 gacgataaag gccgtctgac gggcg
//
LOCUS       DAZFFI010000585          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFFI010000585 DAZFFI010000000
VERSION     DAZFFI010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.589"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..>314
                     /locus_tag="ACHJ12_005643"
     CDS             71..>314
                     /locus_tag="ACHJ12_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO4993224.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQN"
BASE COUNT           78 a           65 c           92 g           79 t
ORIGIN      
        1 gaacagcaga aaactgaagg ttaccggggg accgggtacg acatctcagg tctggttcag
       61 gagaatgggc atgcttgaac gcctgaaaag gttatttcct gtgaagacag aatgctctga
      121 tgtccggacg gatattcccg gggaaacggt gccggagggg tattttacac cattgccggc
      181 tgatgtactg gtatccacac cgttcaggaa aaggtgtctg aaacagttat gggaaaacag
      241 ttccatgccg gtggatatgt atcagcgcct ttgtcttgat cctgtgcatc agttgttgtc
      301 tgtcagtcag aatc
//
LOCUS       DAZFFI010000586          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFFI010000586 DAZFFI010000000
VERSION     DAZFFI010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.590"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..130
                     /locus_tag="ACHJ12_005644"
     tRNA            54..130
                     /locus_tag="ACHJ12_005644"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHJ12_005645"
     tRNA            173..248
                     /locus_tag="ACHJ12_005645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT           81 a           69 c           80 g           78 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcacg gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaag
//
LOCUS       DAZFFI010000587          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFFI010000587 DAZFFI010000000
VERSION     DAZFFI010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.591"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(164..>308)
                     /locus_tag="ACHJ12_005646"
     CDS             complement(164..>308)
                     /locus_tag="ACHJ12_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO4993225.1"
                     /translation="FYRARETDGSASQTFDSGSQGQIQGKITGKDHRCAVGTKMQNGT
                     FCM"
BASE COUNT           62 a           98 c           60 g           88 t
ORIGIN      
        1 tgatgatctt tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct
       61 cactaccatg gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag
      121 atcccggtgg gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc
      181 cgttttgcat ctttgtgcct acggcacacc gatgatcttt ccctgtgatc tttccctgaa
      241 tctgcccctg agaacctgaa tcaaacgtct gactcgcaga accatctgtt tctcgcgctc
      301 tgtagaac
//
LOCUS       DAZFFI010000588          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFFI010000588 DAZFFI010000000
VERSION     DAZFFI010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.592"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHJ12_005647"
     CDS             <1..>306
                     /locus_tag="ACHJ12_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993226.1"
                     /translation="TAKQNADTLKGEADSAVKALPDTAAEKGGLQGRVDALDGIQVPE
                     VNDQDGNGKADAEEAAAAVAAATEKVEAAEAADKAAKDKLAELNADKLITPEEKAQL"
BASE COUNT           84 a           67 c          122 g           33 t
ORIGIN      
        1 aaacggcgaa gcagaatgct gacaccctga agggtgaggc agacagtgcc gtgaaggcgt
       61 tgccggatac agccgcggag aaaggtggcc tgcagggtcg tgtggatgca ctggatggta
      121 tccaggtacc ggaagtgaac gatcaggacg gcaacggcaa ggcggatgca gaggaagcgg
      181 cagcagcggt ggcggcggcc acggagaaag tggaagcggc agaagcggcg gacaaggctg
      241 cgaaggacaa gcttgcagag ctgaatgcag acaagctgat cacgccggaa gagaaggcgc
      301 agctgg
//
LOCUS       DAZFFI010000589          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFFI010000589 DAZFFI010000000
VERSION     DAZFFI010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.593"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..111
                     /locus_tag="ACHJ12_005648"
     CDS             <1..111
                     /locus_tag="ACHJ12_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4993227.1"
                     /translation="MPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT           84 a           57 c           91 g           69 t
ORIGIN      
        1 atgccggtgc cttacggtga aatgctggtt ggttcacgac ggatatccca ggatatcagc
       61 acccgtgatg agggaggcga cgggaaagtg gtggttatcg ggcggggatg aaaataaaaa
      121 aatcccgcag agttagcgga gctgcgggag agaacgatga agattaactt tatggagtta
      181 tttttcaggc atcaaaaaag taacgcagcg tcattattgc ggctacaggc aattgccgga
      241 aatgtgaaga gtttcagaaa ttttattccg tcatgacaca ggcaccctcc ggggtgcctg
      301 t
//
LOCUS       DAZFFI010000590          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFFI010000590 DAZFFI010000000
VERSION     DAZFFI010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.594"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..178
                     /locus_tag="ACHJ12_005649"
     CDS             <1..178
                     /locus_tag="ACHJ12_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4993228.1"
                     /translation="VIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSA
                     IKNMTVEQLLKIHC"
     gene            189..>297
                     /gene="dicB"
                     /locus_tag="ACHJ12_005650"
     CDS             189..>297
                     /gene="dicB"
                     /locus_tag="ACHJ12_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4993229.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKY"
BASE COUNT           91 a           56 c           70 g           80 t
ORIGIN      
        1 ggttatccgt ctcgacgaac acggcaatat caaattcact gaaaccgttc cggacggcgc
       61 aatggtctgc accctggata ccttccagtt ttatctggag aaacaaggct ggactcttgt
      121 aaaccggagc gcaattaaaa atatgactgt ggagcaatta ctaaaaattc attgttgagg
      181 acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc tgaagggtgt tttgatattg
      241 gtgttctgct cagtaaccgg gagtttactg aagatgccat taagatgaga aaatatg
//
LOCUS       DAZFFI010000591          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFFI010000591 DAZFFI010000000
VERSION     DAZFFI010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.595"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>295)
                     /locus_tag="ACHJ12_005651"
     CDS             complement(<1..>295)
                     /locus_tag="ACHJ12_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO4993230.1"
                     /translation="KALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLN
                     ELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLK"
BASE COUNT          109 a           66 c           36 g           84 t
ORIGIN      
        1 tgttttaacc attcccgtaa attaatatac attacaccag agttaaaata gctaccgtta
       61 aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac atcaattacc
      121 actgccccat attcatcttt aaactctaat gctattaact cgttcaatga acccttacag
      181 acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata atcaaatgaa
      241 agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc cttca
//
LOCUS       DAZFFI010000592          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFFI010000592 DAZFFI010000000
VERSION     DAZFFI010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.596"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>293)
                     /locus_tag="ACHJ12_005652"
     CDS             complement(<1..>293)
                     /locus_tag="ACHJ12_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4993231.1"
                     /translation="IIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRD
                     KVFLLEQNPRLFLGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGL"
BASE COUNT           82 a           85 c           65 g           61 t
ORIGIN      
        1 agtccgcggg gatgatgccg gataaaacgg tcaaacccga aacgtccggc tgcattaatc
       61 atatccacat ccgacaggct tcccggcaga cgcgaaagct gcagattttc acgcagagtt
      121 cccagaaaaa gccggggatt ctgttccaga agaaacactt tatcccggac aaaacagggg
      181 tccagttgct gcatcgcaac cccgtcaaga gtaatttgtc ccctgttctg acggtacagc
      241 cccaccatca ttttcagcag cgtcgattta ccacaaccta ttgagccaat aat
//
LOCUS       DAZFFI010000593          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFFI010000593 DAZFFI010000000
VERSION     DAZFFI010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.597"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>293
                     /locus_tag="ACHJ12_005653"
     CDS             <1..>293
                     /locus_tag="ACHJ12_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO4993232.1"
                     /translation="ASRETVSRVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPV
                     TCRDAEILDPRYLDMVADACMSRNRTISQTARDILCEIKNDPGMRHYHSHISQL"
BASE COUNT           72 a           66 c           94 g           61 t
ORIGIN      
        1 gcctccaggg agactgtcag ccgggtgctg ctgatgctgc ggatcacggg ctggctgagt
       61 ctgtgcaaac gggtgcgtga tgagaaaggg cgggtacggg gcaatattta tgcccagcac
      121 gatgaaccgg tgacgtgccg ggatgctgaa atactggatc cacgttatct ggatatggtg
      181 gcagatgcct gtatgagccg gaaccggacc ataagtcaga cggcccggga tattctgtgc
      241 gaaataaaaa atgatccggg aatgcgtcat tatcacagcc atatcagtca act
//
LOCUS       DAZFFI010000594          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFFI010000594 DAZFFI010000000
VERSION     DAZFFI010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.598"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>292
                     /locus_tag="ACHJ12_005654"
     rRNA            <1..>292
                     /locus_tag="ACHJ12_005654"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           67 a           67 c           95 g           63 t
ORIGIN      
        1 gtgcaatggc ataagccagc ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag
       61 gtcatagtga tccggtggtt ctgaatggaa gggccatcgc tcaacggata aaaggtactc
      121 cggggataac aggctgatac cgcccaagag ttcatatcga cggcggtgtt tggcacctcg
      181 atgtcggctc atcacatcct ggggctgaag taggtcccaa gggtatggct gttcgccatt
      241 taaagtggta cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc ta
//
LOCUS       DAZFFI010000595          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFFI010000595 DAZFFI010000000
VERSION     DAZFFI010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.599"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..>291)
                     /locus_tag="ACHJ12_005655"
     CDS             complement(45..>291)
                     /locus_tag="ACHJ12_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016808260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO4993233.1"
                     /translation="SGGGGGLFGQIKGYLQDGVVLFGLVLSAVAFVKVAEAALSTFSE
                     VRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASIIL"
BASE COUNT           86 a           96 c           66 g           43 t
ORIGIN      
        1 agacgatcgg gcagaaaacg aatggtctgc atggggatgt ctccttacag aatgatggag
       61 gccgacttgc ccagcagcca gataatggcg accagcagaa caacaccaac caccacgatg
      121 gcaccaaact gcccccatcc ggccttcccg tcacgaacct ctgagaaggt ggaaagcgcc
      181 gcttccgcca ccttcacaaa agcaactgca ctgagcacaa gaccaaacag cacaacacca
      241 tcctggagat agcctttgat ctgtccgaaa agtccaccac ctccgccgga t
//
LOCUS       DAZFFI010000596          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFFI010000596 DAZFFI010000000
VERSION     DAZFFI010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.601"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>289)
                     /locus_tag="ACHJ12_005656"
     CDS             complement(<1..>289)
                     /locus_tag="ACHJ12_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4993234.1"
                     /translation="SELHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLV
                     DGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPADQDSSCWIRVAQAWAG"
BASE COUNT           41 a          105 c           78 g           65 t
ORIGIN      
        1 tgcctgccca cgcctgtgcc acacggatcc agcacgaact gtcttggtct gccgggttat
       61 aacggtccca gttgaacttc acccgcacgc gaccatgttc gtcgcagaag atttcctcgc
      121 ctgccggtcc tgtcacgaca gcgctctgcg ggccgtcgac cagcggtttc agcaacggct
      181 gtggtcgcca tgttctgtct gccgggatca ccgcaaaatg gttctccagc gccgtccccg
      241 ctccctgccg tcctggcacc gcctgtggct gttcgccgtg cagttcact
//
LOCUS       DAZFFI010000597          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFFI010000597 DAZFFI010000000
VERSION     DAZFFI010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.602"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>288
                     /locus_tag="ACHJ12_005657"
     CDS             <1..>288
                     /locus_tag="ACHJ12_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4993235.1"
                     /translation="VAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQ
                     KRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLA"
BASE COUNT           73 a           79 c           82 g           54 t
ORIGIN      
        1 aggtggcaca tatccgcact cgcgaaacat atggaacccg gcggctccag acggagctgg
       61 cagagaatgg catcatcgtt ggtcgtgacc gactggcacg tcttcgtaag gagctaaggc
      121 tacgctgtaa gcagaaacgc aagttcagag cgactacgaa ctcgaaccac aatctgccag
      181 ttgcgccaaa tctgctgaac cagacgttcg ctcctacagc accaaatcag gtctgggtgg
      241 cggacctgac gtatgttgcc acacaggagg gatggttgta cctcgctg
//
LOCUS       DAZFFI010000598          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFFI010000598 DAZFFI010000000
VERSION     DAZFFI010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.603"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           84 a           56 c           57 g           86 t
ORIGIN      
        1 atatttatta aaattccagt tcttcttttt ctgattacag aaggcaaagt ggcaattacg
       61 catagtttcc cgataaagac gcgatagcga catcccgcat aaggcatttt tctctttatc
      121 tttgtacggt acttcatgga acagagtttt tgaccttgcg aatcgtgatg tctgttgggg
      181 ggcaacttgc tcactgaagc gtgagactcg attaagcgca cgaaacacag aaatcaaaaa
      241 acccggtcac ttttttacaa ggtaaccggg taaaaataat ttt
//
LOCUS       DAZFFI010000599          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFFI010000599 DAZFFI010000000
VERSION     DAZFFI010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.604"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>282)
                     /locus_tag="ACHJ12_005658"
     CDS             complement(<1..>282)
                     /locus_tag="ACHJ12_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4993236.1"
                     /translation="REGERAAADGSITGTPRNSRIASFWMEGPAAAFQTWEQLIFKLL
                     AAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSELLEQRAEPFPR"
BASE COUNT           46 a           93 c           73 g           70 t
ORIGIN      
        1 ccggggaaac ggctcggcac gctgttcaag cagttcactt ttacgctgtt ccgtggctga
       61 acggggcaga tagggccgcc cgatatcggt gttcaccacc gctttcaggg tctcttcact
      121 gccggttcgc tcatactctt cttctgccgc cagcagctta aaaatcagtt gttcccaggt
      181 ctgaaacgcc gcagccggcc cctccatcca gaaacttgct attctggaat ttcggggggt
      241 tccggtgata ctgccatccg ccgctgcccg ttcgccttcc cg
//
LOCUS       DAZFFI010000600          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFFI010000600 DAZFFI010000000
VERSION     DAZFFI010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.605"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHJ12_005659"
     CDS             <1..>281
                     /locus_tag="ACHJ12_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993237.1"
                     /translation="KSVPAHDESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLE
                     TYRKIRNGEWVHNTYARPLTMSEMVVMFGYVYRESKPCHLAREMGINTKT"
BASE COUNT           68 a           56 c           90 g           67 t
ORIGIN      
        1 aagagtgtac cggcacatga tgagtcatgg gtgataatct gtgatgccct ccttggcagg
       61 ctgatgtgct gcatgtttca gggacgccgt tatcttcaga ttgatgcgga agatgttacg
      121 gggcgcctgg agacgtacag gaaaatccgg aatggtgagt gggtacataa tacgtatgcg
      181 cgacctctga cgatgtccga gatggtggtg atgtttggat acgtgtaccg ggagtcaaag
      241 ccctgccatc ttgcccgtga aatggggata aacacgaaga c
//
LOCUS       DAZFFI010000601          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFFI010000601 DAZFFI010000000
VERSION     DAZFFI010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.606"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>280)
                     /locus_tag="ACHJ12_005660"
     CDS             complement(<1..>280)
                     /locus_tag="ACHJ12_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993238.1"
                     /translation="ITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTST
                     GFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYS"
BASE COUNT           84 a           73 c           51 g           72 t
ORIGIN      
        1 cagaatatgc tggtgacgta tgaatcgtca cgctggcatt actggtataa tcccattgaa
       61 tatttacacc agtcgcataa tttccgattg caacgtaaat atcgtaagta tcaccagatg
      121 tattgaccca ggcaaaattt gtaaaccctg tcgatgtgcg ctgccataaa gcaccagtaa
      181 tccccttcgg attaccatta cctgcacgca aaacaagttc agatatacct gcctgttgag
      241 gtgaccccac gttaaatccc gcgccaccaa tcaacgtaat
//
LOCUS       DAZFFI010000602          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFFI010000602 DAZFFI010000000
VERSION     DAZFFI010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.607"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..170)
                     /locus_tag="ACHJ12_005661"
     CDS             complement(<1..170)
                     /locus_tag="ACHJ12_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO4993239.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLL"
BASE COUNT           66 a           86 c           68 g           58 t
ORIGIN      
        1 agcaggaaca cgccttcggc aaaaccgtca ccggcctcac cggcaggaat atcggtaatg
       61 gccacggcca ccatactgcc gaccaccacc gcagcaccgc tcaggatggt ctgatctccg
      121 gcattcacca gttcaatggt ggtaccgtcc tgtacaaaat ttttcgccat aatgctgttt
      181 ctccggacag cccctgtggg gctgtttttc aggcataaaa aaagcccttt cgggcagtga
      241 ttgtgataac gcggttatca ggccaccgac gaacgcac
//
LOCUS       DAZFFI010000603          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFFI010000603 DAZFFI010000000
VERSION     DAZFFI010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.608"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>275
                     /locus_tag="ACHJ12_005662"
     CDS             <1..>275
                     /locus_tag="ACHJ12_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4993240.1"
                     /translation="PTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLALSMCLSAALR
                     RTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVS"
BASE COUNT           53 a           67 c           89 g           66 t
ORIGIN      
        1 cccacgggat ttgactgtct ggatgaaatg acctgtggcc tgcagcctgg tgacctggtt
       61 ctgctggctg cccgtccgtc aatggggaaa acctctctgg cgctatccat gtgtctcagc
      121 gccgcactgc ggcgaacgga agagagcgtt ctggtattca gcattgagat gccgaaggag
      181 caactgattc tgcgcatgct ttccatgctg gggcgtgtgg aactgaatca tttgcgcagt
      241 ggcaacatgg atgatgagga ctggctgagg gtgtc
//
LOCUS       DAZFFI010000604          271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFFI010000604 DAZFFI010000000
VERSION     DAZFFI010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.609"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..195)
                     /locus_tag="ACHJ12_005663"
     CDS             complement(<1..195)
                     /locus_tag="ACHJ12_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993241.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSW"
BASE COUNT           54 a           93 c           56 g           68 t
ORIGIN      
        1 ccagctcatt ccggcatact gtggatggtc agtccccaca atgcggtaca cattgatact
       61 gccctgtccc gtccagtagt gttccgtaat aaaatccgac attgccgcaa actctgcggt
      121 atcccatgca accagttgtt catgtgccca ggccggagca ttcgcctgta tccgctctgt
      181 aaatgccccc ttcattcagc tcttctctcc tgcacttttg agataccgat accgtcaggc
      241 cacggcataa cagccgggcc ccccctgcct t
//
LOCUS       DAZFFI010000605          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFFI010000605 DAZFFI010000000
VERSION     DAZFFI010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.610"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHJ12_005664"
     CDS             complement(<1..191)
                     /locus_tag="ACHJ12_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4993242.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFV"
     gene            complement(181..>268)
                     /locus_tag="ACHJ12_005665"
     CDS             complement(181..>268)
                     /locus_tag="ACHJ12_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4993243.1"
                     /translation="TGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT           96 a           57 c           41 g           74 t
ORIGIN      
        1 attacgaaca ttgacaatag caaagcaacc ccattaagtg tcatatttga tggcacttgc
       61 tgaagtccca gcgcgtttcg gacaataaca aaaacaatcg agaatttaat aaaacatgtc
      121 ccggatgcta ttataaacgg caatagtgta aataatgata atatcgctat cagcgaaatg
      181 ctatttgaca ttattattcc ctgataacca agaatgaatt tctacgccca atttatcatc
      241 cacttcaaca agttcgccat agccagtt
//
LOCUS       DAZFFI010000606          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFFI010000606 DAZFFI010000000
VERSION     DAZFFI010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.611"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>267
                     /locus_tag="ACHJ12_005666"
     CDS             <1..>267
                     /locus_tag="ACHJ12_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4993244.1"
                     /translation="AAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGG
                     QNPFLIMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDS"
BASE COUNT           59 a           73 c           78 g           57 t
ORIGIN      
        1 tgctgctgag cagagtctga gccgacaggc gctggctgca caaaaagccg ggatatcagt
       61 cggacagtat aaggctgcca tgcgcacact gcccgcacag ttcacggata ttgtcactca
      121 gcttgccggt ggtcagaatc ccttccttat catgctgcag cagggggggc agatcagcga
      181 ttcattcggt ggaccgctca gcctgcttac cctgctgaag gaggaacttc tcgggatcag
      241 ggatgcctct gaatcatcag aggattc
//
LOCUS       DAZFFI010000607          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFFI010000607 DAZFFI010000000
VERSION     DAZFFI010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.612"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>263)
                     /locus_tag="ACHJ12_005667"
     CDS             complement(<1..>263)
                     /locus_tag="ACHJ12_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO4993245.1"
                     /translation="VSGRLDKAPMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVR
                     FGYADDTTYRLVDRNEHWRDFHTPRLNKLVTEVAPVSVTDAGTG"
BASE COUNT           62 a           85 c           59 g           57 t
ORIGIN      
        1 ccggttccgg catccgtgac gctcaccggg gcaacttctg tcaccagttt gttcagccgg
       61 ggagtatgaa aatcacgcca gtgctcatta cggtcaacaa ggcgatatgt cgtatcgtcc
      121 gcataaccaa aacgcaccgt attgtagctg ccgttatgct taacccccgc cacatactgc
      181 ggagaaatca gtgttgccac ccccagtatc tggtccacaa cactgaaatc catcatcgga
      241 gccttgtcca gccgcccact gac
//
LOCUS       DAZFFI010000608          257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFFI010000608 DAZFFI010000000
VERSION     DAZFFI010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.614"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            4..>257
                     /locus_tag="ACHJ12_005668"
     CDS             4..>257
                     /locus_tag="ACHJ12_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4993246.1"
                     /translation="MNISQLAALSGAHRQTVAARVKNISPAGGHESNLKLYRLTDILA
                     ELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVS"
BASE COUNT           70 a           67 c           64 g           56 t
ORIGIN      
        1 catctgaata tttcccaact ggccgcatta tccggtgctc atcgacagac tgtcgcggct
       61 cgggtaaaaa acataagccc agccggtggt catgagagca atctcaaact gtaccgactg
      121 acagatatcc ttgccgagct gatgaaagct cctctgcctg tagataacga ggaaatggat
      181 cctcatgcgc gtaaagcatg gtaccagtca gaacgtgacc gactgaaatt tgagcaggaa
      241 accggtctgc tcgtgtc
//
LOCUS       DAZFFI010000609          250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFFI010000609 DAZFFI010000000
VERSION     DAZFFI010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.615"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..222
                     /locus_tag="ACHJ12_005669"
     CDS             <1..222
                     /locus_tag="ACHJ12_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4993247.1"
                     /translation="GGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMK
                     AVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT           57 a           48 c           97 g           48 t
ORIGIN      
        1 ggtggttatg tgggtggtgc cggaagtccg gcgcagatgc ggcgggcgga aggtattaat
       61 tttaatcaga acaatcacgt ggtgattcag aacgacggta tcaacggaca ggcggggccg
      121 cagctgatga aggcggtgta tgacatggcc cgcaaggggg cgcaggatga gctccggctg
      181 cagttgcgtg atggcggtat gttatcaggg agcgggcgat gaaaaccttt cgctggaaag
      241 tgaagccgga
//
LOCUS       DAZFFI010000610          250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFFI010000610 DAZFFI010000000
VERSION     DAZFFI010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.616"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(92..>250)
                     /locus_tag="ACHJ12_005670"
     CDS             complement(92..>250)
                     /locus_tag="ACHJ12_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO4993248.1"
                     /translation="DEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSE
                     NDGPGGYA"
BASE COUNT           45 a           85 c           62 g           58 t
ORIGIN      
        1 gtttccccca ttgccaccac attgccggca ccagtggtct gacgaccggg ctgcataata
       61 gccgccccga gatccagtgg tttatgtgcc atcacgcata tcctcccggc ccgtcattct
      121 cagagggcgt aaacagtgtg taacgaccgt cgaacctgac ccgtaccgta cccgtgggcc
      181 cctgacgctg cttgctgaca atgatttctg cctccccctt tgccagtgag tcggggttat
      241 agacctcgtc
//
LOCUS       DAZFFI010000611          249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFFI010000611 DAZFFI010000000
VERSION     DAZFFI010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.617"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>249)
                     /locus_tag="ACHJ12_005671"
     CDS             complement(<1..>249)
                     /locus_tag="ACHJ12_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4993249.1"
                     /translation="RLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQ
                     LTAVVSPDGLESRREYDEPGRLVSETSRSGETVRYRYD"
BASE COUNT           33 a           93 c           64 g           59 t
ORIGIN      
        1 tcatcgtagc ggtagcgtac tgtctccccg ctgcgcgatg tctccgatac cagcctgccc
       61 ggttcatcat attcccggcg gctctccagc ccgtccgggg acaccaccgc cgtcagctgg
      121 ttcccgtcgt tatagtaaaa tttcgtctcc cgcccgtccg gtgtggtgat gtccgtgata
      181 tcgccggaca ccacattcag accgtactct gtccgccgtc ccgccgcgtc cgtctgcgcc
      241 gtgagcctt
//
LOCUS       DAZFFI010000612          249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFFI010000612 DAZFFI010000000
VERSION     DAZFFI010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.618"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            150..>249
                     /gene="yrhD"
                     /locus_tag="ACHJ12_005672"
     CDS             150..>249
                     /gene="yrhD"
                     /locus_tag="ACHJ12_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO4993250.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDF"
BASE COUNT           87 a           43 c           42 g           77 t
ORIGIN      
        1 ttcgtttagt gatttctctt cattcttaaa cgaaatcatg gacagttgtt cataataatc
       61 aaaaggaaac aaaaatcatt gtaaaccatg ccatcttttt agagattacc agcagaaaat
      121 tccttgttcg aaatttagca gtgcaagcta tggaaaaact tacaacggaa ctacactcct
      181 taagtgagat ggaccgaaga catgtagtct ctattttagc cgaaatagct aatggctatg
      241 atgatttta
//
LOCUS       DAZFFI010000613          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFFI010000613 DAZFFI010000000
VERSION     DAZFFI010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.619"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>245)
                     /locus_tag="ACHJ12_005673"
     CDS             complement(<1..>245)
                     /locus_tag="ACHJ12_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFO4993251.1"
                     /translation="NPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDG
                     GNTRYQILTELWKETGDPRFYRIHVLFKPWPGRLQCV"
BASE COUNT           54 a           65 c           66 g           60 t
ORIGIN      
        1 atcacacact ggagtcgacc cggccagggt ttaaacagca catgaatacg gtaaaaacgc
       61 ggatcccctg tttctttcca gagttccgtc agtatctggt aacgggtgtt tcccccgtca
      121 ctgaagatat acacatccgg ctcactgtcc ggcatacggg tgactttggg caccgtatcc
      181 agcccgcggg catgaatgga atttttgata tcgtcataac gcggattgcg cgtggtgcgc
      241 gggtt
//
LOCUS       DAZFFI010000614          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFFI010000614 DAZFFI010000000
VERSION     DAZFFI010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.denovo.620"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>245
                     /locus_tag="ACHJ12_005674"
     CDS             <1..>245
                     /locus_tag="ACHJ12_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO4993252.1"
                     /translation="GGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAP
                     APSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYG"
BASE COUNT           48 a           73 c           79 g           45 t
ORIGIN      
        1 ggcggttcac tgagctatct gggcgaggat accgccgaac agctggagtt gatgcgtagc
       61 gccttccggg ttatccggac ggtacgggaa aaacatgcct gtactcagtg cgatgccatc
      121 gtgcaggcac ctgcaccttc gcggcccatc gagcggggta tcgccggacc ggggctgctg
      181 gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc tgtatcgcca gtcagaaata
      241 tacgg
//
LOCUS       DAZFFI010000615         8802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.guided.159, whole genome shotgun sequence.
ACCESSION   DAZFFI010000615 DAZFFI010000000
VERSION     DAZFFI010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.guided.159"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..287
                     /locus_tag="ACHJ12_005675"
     CDS             <1..287
                     /locus_tag="ACHJ12_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4993253.1"
                     /translation="NWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQ
                     SAGNRRIEIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            358..1413
                     /gene="dinB"
                     /locus_tag="ACHJ12_005676"
     CDS             358..1413
                     /gene="dinB"
                     /locus_tag="ACHJ12_005676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4993254.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            1410..1862
                     /locus_tag="ACHJ12_005677"
     CDS             1410..1862
                     /locus_tag="ACHJ12_005677"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4993255.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRGAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            2108..3247
                     /locus_tag="ACHJ12_005678"
     CDS             2108..3247
                     /locus_tag="ACHJ12_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO4993256.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            3244..3858
                     /gene="prfH"
                     /locus_tag="ACHJ12_005679"
     CDS             3244..3858
                     /gene="prfH"
                     /locus_tag="ACHJ12_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO4993257.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(3915..5372)
                     /gene="pepD"
                     /locus_tag="ACHJ12_005680"
     CDS             complement(3915..5372)
                     /gene="pepD"
                     /locus_tag="ACHJ12_005680"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4993258.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            5633..6091
                     /gene="gpt"
                     /locus_tag="ACHJ12_005681"
     CDS             5633..6091
                     /gene="gpt"
                     /locus_tag="ACHJ12_005681"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4993259.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            6183..7427
                     /gene="frsA"
                     /locus_tag="ACHJ12_005682"
     CDS             6183..7427
                     /gene="frsA"
                     /locus_tag="ACHJ12_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4993260.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            7485..7886
                     /gene="crl"
                     /locus_tag="ACHJ12_005683"
     CDS             7485..7886
                     /gene="crl"
                     /locus_tag="ACHJ12_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4993261.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(7925..>8802)
                     /gene="phoE"
                     /locus_tag="ACHJ12_005684"
     CDS             complement(7925..>8802)
                     /gene="phoE"
                     /locus_tag="ACHJ12_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4993262.1"
                     /translation="FKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
BASE COUNT         2156 a         2145 c         2296 g         2205 t
ORIGIN      
        1 acaactggaa cctttcgggt gaccgcgcgc tttcggctcg tcgggtgctg gaagaggccg
       61 gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggatt
      121 ccaaaaatcc gcaaagcgcg ggcaaccggc gcattgagat tatggtgctg accaaaagtg
      181 cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg
      241 tgcaaaagct ggataagcag caggtgcttt cgcagcgaac gcgttaaatg ctgaatcttt
      301 acgcatttct caaaccctga aatcactgta tactttacca gtgttgagag gtgagtaatg
      361 cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat
      421 cccgccctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc
      481 agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc gacagggatg
      541 gcgctcaaat tatgcccgca tctcaccttg cttccggggc gctttgacgc ctacaaagaa
      601 gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgtattga accattgtca
      661 ctggatgagg cttatctcga cgtcaccgat agcgtccatt gccacggttc tgcgaccctc
      721 atcgcccagg aaatccgcca gacgattttc agcgagctgc aactgacagc gtctgcgggc
      781 gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag
      841 tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccgct ggcaaaaatc
      901 cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt
      961 gatgtacaaa agtgtgatct ggtgatactg cttaaacgct ttggcaaatt tggccgcatt
     1021 ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttgcgaaaa
     1081 tccgtcggcg tggaacgcac gatggcggaa gatatccacc actggtctga atgtgaagcg
     1141 attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta
     1201 ctgattgccc gccagggggt gaaattaaag ttcgacgatt ttcagcaaac cactcaggag
     1261 cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat
     1321 gaacgccgcg gcgggcgcgg tgtgcgactg gtggggctgc atgtgacgtt gcttgacccg
     1381 caaatggaaa gacaactggt gctgggatta tgaataacat acaaataaga gactatcagc
     1441 ctggcgattt tcagcaacta tgcgctattt tcattagagc ggttacgatg actgccagtc
     1501 agcattattc accacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg
     1561 agaaactcgc gaaatcacaa gtgcggggtg cagtcattaa tgcaaaactg gtcggcttta
     1621 ttacctgcgt tgaacactat atcgatatgt tatttgttga ccctgaatac acccgccgtg
     1681 gggttgccag cgctttgtta aaacctttga ttaagtctga atccgaactt acggtggacg
     1741 caagcataac cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcaac
     1801 gcgttgaatg tcggggagag tggtttatta atttttatat gcgttataaa cagcaacatt
     1861 aaatccagct tgcaatgaaa ataacgcaag cctagtatgt gcgcgttttc ttattccaca
     1921 aactgcaagg aggtaaatca tgacaaatcc tttatcaatg actctttgca gacctttcca
     1981 ggattaattc tttttttctt gccctggatt cgtctgccat ttcctgattt tttaattgtt
     2041 actctcaata attcaagtcg caacacatgc aacttgaagt atgacgagtc tatttacgga
     2101 atggtatatg ggcaattata ttcgtccctt atccgatgcg gtatttacca tcgcatctga
     2161 tgacctgtgg atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa
     2221 catgcagcgc gtagtgggga tgccagattt acatcccgga cgcggctatc cgattggcgc
     2281 agcgttcttc tctgttggtc gtttttatcc ggcactggtc ggcaatgata tcggctgcgg
     2341 tatggcgcta tggcaaacag atattctcac tcgcaaatac aacgccgata agtttgaaaa
     2401 gcgattatca gatctggatg acgttgctga agaaagctgg ctggaggaaa acctgccgtc
     2461 agcatttgca cagcatccgt ggcgcagctc gcttggctct atcggtggcg gcaatcattt
     2521 cgctgaactg caacaagttg atcaaatcat caacgctgaa ctgtttgcac tggcaggtct
     2581 ggatgcacaa catctgcaac tgttggttca tagcggctcg cggggggtgg ggcagtctat
     2641 tttacagcgg catattgcct cgttctcgca tcatggtttg cctcaaggca gtgacgacgc
     2701 gctacggtat attgcggaac atgacgatgc gctggcgttt gcgtgtatta atcgccagat
     2761 gatcgctttg cgcataatgc aacaagttaa ggccacggga agtccggttc tggatgtggc
     2821 gcataacttt gttagcgcgt gtcaaatcgg cgatcaacag ggctggttgc atcgtaaagg
     2881 tgccacgccg gatgacaacg gtctggtgat tattcccggt tcacgtggtg attactcctg
     2941 gattgtacag cctgttgcga acgaaaaaac gctccactcg ctggcgcatg gggcagggcg
     3001 taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacgg cgacgcaact
     3061 ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tctttgaaga
     3121 agcgccacaa gcctataaat cggctgaaag cgtggtgcaa tgtctggtgc aggccggttt
     3181 aattattcct gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa
     3241 aggatgatct tgctacaact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg
     3301 aaaaaggcgc tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg
     3361 ttggaaacag aaacgggccg ctactctgac acgctgcgtt cggcgctgat ttctctggat
     3421 ggcgataacg catgggcatt aagcgaaagc tggtgtggca ctattcagtg gatttgtccg
     3481 agtccgtatc ggcctcatca tgggcgcaaa aactggtttc tgggcattgg gcgttttacc
     3541 gctgatgagc aggaacaatc ggatgcaatc cgttatgaga cgttgcgttc gtcggggccg
     3601 ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt
     3661 attagcgtga aggttcagtc agagcgtagt cagcatgcta acaagcggct ggcacgattg
     3721 ctgattgcct ggaagctgga gcagcagcaa caggaaaata gcgcggtgct gaaatcgcag
     3781 cggcgaatgt tccatcacca gattgaacgt ggcaacccga gacggacatt tacagggatg
     3841 gcttttatcg aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc
     3901 agcaaatcaa ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag
     3961 taatgaccta cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt
     4021 ggcccgatag aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg
     4081 cccgcgtgga taatctggat gttcggcgtt ttgttgaaca gacgctgata ggtttcacgt
     4141 accagatgca tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct
     4201 tcggttttcg cgccagccag tttacccagc gaatccagca tgctcacgac gtagtcttta
     4261 ccgctgtcga tcagtgaacg gatcagacag tgaatttcta cgttattatc agtcatggtc
     4321 accacaccga cgttcaggga ggtttcgacc acaccttttg ccacgtctga gttgcggatc
     4381 acaccgttcg gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg
     4441 gcagctttat cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc
     4501 agctcgtttt tcaggatctc ctgataggta ttcaccagag atttcaggac gtcgacttta
     4561 tcagctgcga cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg
     4621 ccgttgaaat cgataaggcg cagatccagt tcttctgcat gacccgccag gaagcgcacc
     4681 agcagtttgt tggcattacc caggccaacg tggatttccc cgccggagtg accgcctttt
     4741 agacctttta aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa
     4801 tgcaggttgg aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct
     4861 tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca
     4921 ccgtccatac cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg
     4981 ttttcgtcag ccagaaccgc cagcgcagac gccataccaa tgccgttatc tgcacccagc
     5041 gtggtgccgc gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg
     5101 aagtcatgca cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg
     5161 accggtttac gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc
     5221 tgatcgcgtt cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt
     5281 tgctcttcat gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc
     5341 ggctgtggag ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa
     5401 aacagtattg caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga
     5461 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     5521 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     5581 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     5641 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     5701 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg
     5761 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     5821 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     5881 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     5941 gtatccaaaa gcacattttg tgactatctt cgcaaaaccg gctggtcgtc cgctggttga
     6001 tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt
     6061 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     6121 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     6181 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     6241 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg
     6301 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     6361 gcattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac
     6421 gtaccgatga tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg
     6481 agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac
     6541 tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc
     6601 tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg
     6661 ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc
     6721 ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt
     6781 gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc
     6841 cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga
     6901 agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac
     6961 cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg
     7021 tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag
     7081 ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg
     7141 ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttgcgc gtggagttga
     7201 atcgctattc attaaaagtg caaggattgc tgggacgtcg ctgcccaacg ccaatgttat
     7261 caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat
     7321 catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca
     7381 aaggtcttca ggaaatcacc gactggatcg aaaaacgttt gtgttaaaaa tttgctaaat
     7441 tttaccaatt tggtaaaaca gttgcatcac aacaggagat tgcaatgacg ttaccgagtg
     7501 gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag
     7561 gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac
     7621 cggcaccgga agtgcgtgag ttctggggct ggtggatgga gctggaagcg caggaatccc
     7681 gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc
     7741 cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga
     7801 agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg
     7861 acgagccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata
     7921 tctattaaaa ctgataggtc atgccaaccg cgacaatatc atcattatta atattcaatt
     7981 tgttatcgct atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga
     8041 aataatatgt agccccgaca tcgatataat tgaccagatc ttcatcaccg ataccttcaa
     8101 tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt
     8161 attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca
     8221 ttttgcgtgt ttcagaataa aaagttgcca gataaatatt attggcatcg tatttcagac
     8281 ctgtagccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg
     8341 tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg
     8401 tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc
     8461 cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat
     8521 acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc
     8581 caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac
     8641 gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg agacgcgttt
     8701 tttgctgtgc ggtatcactc tccgctttat ttccggcaaa ctccgcttcc caccgaccat
     8761 aaccagtcag ttgatcgtta atttgtgttt cacctttaaa ac
//
LOCUS       DAZFFI010000616         8387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.guided.163, whole genome shotgun sequence.
ACCESSION   DAZFFI010000616 DAZFFI010000000
VERSION     DAZFFI010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.guided.163"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            354..2162
                     /gene="frdA"
                     /locus_tag="ACHJ12_005685"
     CDS             354..2162
                     /gene="frdA"
                     /locus_tag="ACHJ12_005685"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4993263.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2155..2889
                     /gene="frdB"
                     /locus_tag="ACHJ12_005686"
     CDS             2155..2889
                     /gene="frdB"
                     /locus_tag="ACHJ12_005686"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4993264.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2900..3295
                     /gene="frdC"
                     /locus_tag="ACHJ12_005687"
     CDS             2900..3295
                     /gene="frdC"
                     /locus_tag="ACHJ12_005687"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4993265.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3306..3665
                     /gene="frdD"
                     /locus_tag="ACHJ12_005688"
     CDS             3306..3665
                     /gene="frdD"
                     /locus_tag="ACHJ12_005688"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4993266.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3728..4861
                     /locus_tag="ACHJ12_005689"
     CDS             3728..4861
                     /locus_tag="ACHJ12_005689"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4993267.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4950..5483
                     /gene="blc"
                     /locus_tag="ACHJ12_005690"
     CDS             4950..5483
                     /gene="blc"
                     /locus_tag="ACHJ12_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4993268.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5480..5797)
                     /gene="sugE"
                     /locus_tag="ACHJ12_005691"
     CDS             complement(5480..5797)
                     /gene="sugE"
                     /locus_tag="ACHJ12_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4993269.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5972..6118)
                     /gene="ecnB"
                     /locus_tag="ACHJ12_005692"
     CDS             complement(5972..6118)
                     /gene="ecnB"
                     /locus_tag="ACHJ12_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4993270.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6229..6354)
                     /gene="ecnA"
                     /locus_tag="ACHJ12_005693"
     CDS             complement(6229..6354)
                     /gene="ecnA"
                     /locus_tag="ACHJ12_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4993271.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6406..6972)
                     /gene="efp"
                     /locus_tag="ACHJ12_005694"
     CDS             complement(6406..6972)
                     /gene="efp"
                     /locus_tag="ACHJ12_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4993272.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7014..8042
                     /gene="epmB"
                     /locus_tag="ACHJ12_005695"
     CDS             7014..8042
                     /gene="epmB"
                     /locus_tag="ACHJ12_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4993273.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2095 a         2146 c         2178 g         1968 t
ORIGIN      
        1 ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg
       61 aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc
      121 agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata
      181 atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag
      241 actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag
      301 gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa
      361 cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg
      421 ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc
      481 gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct
      541 tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg
      601 tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg tgggggtgcc
      661 catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg
      721 agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga
      781 cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg
      841 ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc acgctggtgc
      901 agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca
      961 acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc
     1021 cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc
     1081 tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt
     1141 atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata
     1201 tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca
     1261 acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac ctcggcgaga
     1321 aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg
     1381 atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg
     1441 aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct
     1501 ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa ctggtggtct
     1561 tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg
     1621 aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc
     1681 aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggtctggct atggaagaag
     1741 gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc
     1801 tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc
     1861 tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg
     1921 caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc
     1981 gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc
     2041 gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg
     2101 gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct
     2161 gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg
     2221 catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc
     2281 tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt
     2341 tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg
     2401 cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat
     2461 ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc
     2521 ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag
     2581 tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt
     2641 ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa
     2701 gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta
     2761 tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct
     2821 gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa
     2881 ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc
     2941 cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt
     3001 tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc
     3061 ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct
     3121 gatcactctg gcggcagctc tgctgcatac caaaacctgg tttgaactgg caccgaaagc
     3181 ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg
     3241 ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc
     3301 ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg
     3361 gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc
     3421 tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg
     3481 cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg
     3541 gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat
     3601 gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa
     3661 tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc
     3721 agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt
     3781 tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca
     3841 acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac
     3901 ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt
     3961 aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gaggctattg ctcgagggga
     4021 aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta aacagtggaa
     4081 tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat tgcaggtgcc
     4141 ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg
     4201 ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc
     4261 tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct tccagccact
     4321 caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg
     4381 atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg aaacttatgg
     4441 tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga
     4501 tatcaacgac aaaacacttc aacaagggat acaactggca caatctcgct actggcaaac
     4561 cggcgatatg tatcagggtc tgggctggga aatgctggac tggccggtaa atcctgacat
     4621 catcgttaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa aagcgattac
     4681 gcccccaact cctgcagtac gcgcatcatg ggtgcataaa acgggggcga ccggcggatt
     4741 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     4801 aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta
     4861 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     4921 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     4981 cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc gtaacagtag
     5041 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     5101 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     5161 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     5221 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     5281 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     5341 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     5401 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     5461 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     5521 ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc acaatgccgg
     5581 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     5641 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acagtaataa
     5701 cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc cataccactt
     5761 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga gaattggggc
     5821 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     5881 ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca aaaaaacaaa
     5941 cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt gctgcaccag
     6001 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
     6061 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
     6121 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
     6181 tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
     6241 gcgagagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca
     6301 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
     6361 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
     6421 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
     6481 aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
     6541 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
     6601 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
     6661 agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
     6721 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
     6781 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
     6841 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
     6901 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
     6961 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
     7021 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
     7081 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
     7141 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
     7201 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
     7261 ttgtcgtcgc ttccggattc tccaccgacc cgctggaaga acagcacagc gtagtgcctg
     7321 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
     7381 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
     7441 actggcagac tgctcttgag tatgttgctg cgcatccgga actggacgag atgattttct
     7501 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
     7561 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
     7621 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
     7681 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
     7741 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
     7801 acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg atgccgtatt
     7861 acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
     7921 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg
     7981 cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
     8041 aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc aggcctacat
     8101 tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc gtttacgccg
     8161 catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg gaaatttaat
     8221 ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac tctttatata
     8281 gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg gctatttatg
     8341 ttattttaac aactattact accaagccca tttatctggc ttataaa
//
LOCUS       DAZFFI010000617         1730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1801M74112
            SAMN43251493-rid23064223.guided.315, whole genome shotgun sequence.
ACCESSION   DAZFFI010000617 DAZFFI010000000
VERSION     DAZFFI010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251493
            Sequence Read Archive: SRR30292959
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349810.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349810.1
            Genome Coverage       :: 133x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 00:42:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,697
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,419
            CDSs (with protein)               :: 5,419
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 87 of 185
            Pseudo Genes (incomplete)         :: 105 of 185
            Pseudo Genes (internal stop)      :: 32 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251493-rid23064223.guided.315"
                     /serotype="O146:H21"
                     /isolate="1801M74112"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-01"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..1095
                     /gene="stxA2b"
                     /locus_tag="ACHJ12_005696"
     CDS             136..1095
                     /gene="stxA2b"
                     /locus_tag="ACHJ12_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFO4993274.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1108..1371
                     /gene="stxB2b"
                     /locus_tag="ACHJ12_005697"
     CDS             1108..1371
                     /gene="stxB2b"
                     /locus_tag="ACHJ12_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFO4993275.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          482 a          354 c          415 g          479 t
ORIGIN      
        1 ttctaagcaa tcggtcactg gttcgaatcc agtacaacgc gccataatta tttaccaggc
       61 tcgcttttgc gggccttttt tatatctgcg ccgggtctgg tatcgtatta cttcagccaa
      121 aaggaatacc tgtatatgaa gtgtatattg ttaaaatggg tactgtgcct gttactgggc
      181 ttttcttcgg tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat
      241 gtctcttcgt taaatagtat acggacagaa atatcgaccc cacttgaaca tatatctcag
      301 gggaccacat cggtatctgt tattaaccac accccaccgg gcagttattt tgctgtggat
      361 atacgagggc ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa
      421 aataatttat atgtggccgg attcgttaat acggcaacaa atactttcta cagattttca
      481 gattttgcac atatatcagt gcccggtgtg acaactgttt ccatgacaac ggacagcagt
      541 tataccactc tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac
      601 tcactggttt catcatatct ggcgttaatg gagtttagtg gaaatgccat gaccagagat
      661 gcatccagag cagttctgcg ttttgtcact gtcacagcag aagccttacg gttcaggcaa
      721 atacagagag aatttcgtct ggcactgtct gaaactgctc ctgtttatac gatgacaccg
      781 gaagaagtgg acctcacact gaactggggg agaatcagca atgtgcttcc ggagtttcgg
      841 ggagagggtg gtgtcagagt ggggcgaata tcctttaata atatatcagc gatactgggc
      901 acagtggcgg ttatactgaa ttgccatcat cagggggcac gttccgttcg ctccgtgaat
      961 gaagagatac aaccagaatg tcagataact ggcgacaggc cagttataag gctaaacaat
     1021 actttatggg aaagtaatac cgcagctgct tttctgaatc gcagggctca ctctttaaat
     1081 acatccggag aataacggga gttaaatatg aagaagatat ttgtagcggc tttatttgct
     1141 tttgtttctg ttaatgcaat ggcagctgat tgtgcaaaag gtaaaattga gttctctaag
     1201 tataatgaga atgatacatt cacagtaaaa gtggccggga aagagtactg gactaaccgc
     1261 tggaatctgc aaccgctact gcaaagcgca cagttaacag gaatgacggt aacaatcaaa
     1321 tcaaatacct gtgcgtcagg ttcaggattt gctgaagtgc agtttaatta atatcagaag
     1381 cattgctggt ttcgtagtgt gcagcaatgt agttacagtg taatcaatgt cacaattcag
     1441 tcagttgacg gttgcctgtc tggctgagca tttgttaaaa aaatcctgca tgatgaatcc
     1501 ccctgtgcgg aggggcataa tgacagatgt ttggttgcgt attgtatagg caagttgcgg
     1561 attctgtctg gtcattgcag aattcaccgg gaggcacccg gcatcatgct gtatacagag
     1621 attaggcata tatccaggct cctcatcgca ggagcctttt tacatgcaaa aaaagcccgc
     1681 gccgggagac gcgggcagca aggaataaac aacaaaacgt gaagtaatca
//
